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Sample records for asepsis

  1. Asepsis: back to the basics.

    Science.gov (United States)

    Crow, S

    1998-03-01

    Nosocomial infections are the 11th leading cause of death in the United States, cost approximately $4.65 billion yearly--and can be prevented. Patients are at risk for nosocomial infection, especially when they undergo surgical procedures. It is important to know and carry out the principles of asepsis to reduce this risk of infection regardless of where the procedure occurs.

  2. Medical Asepsis. Kit No. 302. Instructor's Manual [and] Student Learning Activity Guide. Health Occupations.

    Science.gov (United States)

    Edwards, Gloria

    This instructor's manual and student learning guide comprise a module on medical asepsis for a secondary-level health occupations program. The six activities in the module cover medical asepsis terms; ways organisms spread; types of medical asepsis; aseptic equipment care; proper handwashing; and procedures for using masks, gloves, and gowns.…

  3. Asepsis and bacteriology: a realignment of surgery and laboratory science.

    Science.gov (United States)

    Schlich, Thomas

    2012-07-01

    This paper examines the origins of aseptic surgery in the German-speaking countries. It interprets asepsis as the outcome of a mutual realignment of surgery and laboratory science. In that process, phenomena of surgical reality were being modelled and simplified in the bacteriological laboratory so that they could be subjected to control by the researcher's hands and eyes. Once control was achieved, it was being extended to surgical practice by recreating the relevant features of the controlled laboratory environment in the surgical work place. This strategy can be seen in the adoption of Robert Koch's bacteriology by German-speaking surgeons, and the resulting technical changes of surgery, leading to a set of beliefs and practices, which eventually came to be called 'asepsis'.

  4. [Asepsis and antisepsis in dental offices in Dakar].

    Science.gov (United States)

    Lo, C M M; Cisse, D; Faye, D; Kane, A W

    2004-09-01

    To evaluate hygiene in dental offices in Dakar, the authors questioned 64 public, private and semi-private dentists. From 62 practitioners who completed their questionnaire, it appeared that the dry-heated sterilizer (poupinel) was 5 times more used than the autoclave; The almost practitioners sayed respecting the sterilisation process and 66% of them said wearing gloves, masks and glasses during interventions. In spite of the hope these results give, this study is limited because it doesn't take into account many aspects of asepsis and antisepsis in dental office which require a rigorous application next to a personal who needs to be better trained.

  5. Does the level of asepsis impact the success of surgically implanting tags in Atlantic salmon?

    DEFF Research Database (Denmark)

    Jepsen, Niels; Boutrup, Torsten S.; Midwood, Jonathan D.;

    2013-01-01

    It is generally recommended that a high level of asepsis be maintained during surgical implantation of electronic tags into fish. However, documentation of a positive effect of asepsis in fish surgery is lacking. To compare the effects of surgical implantation performed under different sanitary...... and fish tagged without regard to aseptic technique. The results demonstrated that there was no detectable difference in survival, growth and healing between the treatments. Thus, this study could not provide evidence supporting the general recommendation of achieving a high level of asepsis during fish...

  6. [Asepsis sowing and tissue culture of Bletilla striata].

    Science.gov (United States)

    Zeng, Songjun; Huang, Xiangli; Chen, Zhilin; Chen, Jiantong; Duan, Jun

    2004-09-01

    The asepsis sowing and tissue culture of Bletilla striata were studied. The results indicated that the embyro culture had highest sprouting percent and plantlets percent when their embryos were mature. The optimal medium for the embryo culture was 1/2 MS. Adding 10% coconut juice can promote embyros sprouting and plantlets formation, 1% active carbon can improve plantlets growing. The best medium for the top of plantlet on culture and multiplication was 1/2 MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L. The best medium of rooting was 1/2 MS + NAA 0.5 mg/L, and 10% banana juice can improve rooting of plantlets.

  7. [Field study of nursing care quality. Interaction and asepsis in clinical practice].

    Science.gov (United States)

    Brataas, H V

    1996-01-01

    Competence as communication skills and as skilled practice of asepsis were studied by observing four nurses while interacting with patients and performing intravenous procedures. Nurses were observed using sterile equipment for methods of intravenous therapy. Asepsis is performed frequently, but through misunderstanding in the learning of asepsis or improper model learning the nurses may establish incorrect routines. When performing procedures, unexpected factors can distract both the expert and the inexperienced, resulting in a failure to apply basic aseptic techniques. The nurses showed an interest in the patient by listening and giving responses. Nurses may control interactions with the patient by using undesirable communication skills which include incomplete sentences, incomplete explanations and closed questions. Asked to evaluate their own behavior, the nurses did not estimate whether or not the appropriate skills were applied in observed situations. To improve the quality of nursing care performance it is recommended to further develop and apply skill training programs.

  8. Above-knee vein harvest for coronary revascularization increases ASEPSIS score.

    Science.gov (United States)

    Akowuah, Enoch; Shrivastava, Vivek; Ponniah, Alan; Jamnadas, Binal; Chilton, Gary; Cooper, Graham

    2006-02-01

    The long saphenous vein may be harvested from the thigh or the lower leg, depending on the operating surgeon's preference. This prospective study compared the incidence of altered wound healing between these two sites in 175 patients undergoing routine coronary artery bypass grafting over a 3-month period. The patients were divided into 3 groups. In group A, the vein harvest site was restricted to above the upper border of the patella. Group B included harvest sites that started at the ankle but extended above the level of the upper border of the patella. In group C, the vein harvest site was restricted to below the upper border of the patella. The wounds were assessed daily using the ASEPSIS scoring system. In group A, significantly more patients (24%) had an ASEPSIS score > 10, compared to group B (3%) and group C (2%). The mean ASEPSIS score was significantly lower in group C than groups A or B, 1.5 +/- 2.4 vs. 6.5 +/- 3.2 or 3.7 +/- 1.7, respectively. The ASEPSIS score is reduced when vein harvest is restricted to below the level of the knee.

  9. [The efficacy of three hand asepsis techniques using chlorhexidine gluconate (CHG 2%)].

    Science.gov (United States)

    da Cunha, Érika Rossetto; Matos, Fabiana Gonçalves de Oliveira Azevedo; da Silva, Adriana Maria; de Araújo, Eutália Aparecida Cândido; Ferreira, Karine Azevedo São Leão; Graziano, Kazuko Uchikawa

    2011-12-01

    The scrubbing of hands and forearms using antiseptic agents has been the standard pre-operative procedure to prevent surgical site infection. With the introduction of antiseptic agents, the need to use brushes for pre-operative disinfection has been questioned and it has been recommended that the procedure be abandoned due to the injuries it may cause to the skin. With the purpose to provide the foundations for the efficacy of pre-operative asepsis without using brushes or sponges, the objective of this study was to evaluate three methods of pre-operative asepsis using an antimicrobial agent containing chlorhexidine gluconate - CHG 2%; hand-scrubbing with brush (HSB), hand-scrubbing with sponge (HSS), and hand-rubbing with the antiseptic agent (HRA) only. A comparative crossover study was carried with 29 healthcare providers. Antimicrobial efficacy was measured using the glove-juice method before and after each tested method. Statistical analyses showed there were no significant differences regarding the number of colony-forming units when comparing HRA, HSB, and HSS techniques (p=0.148), which theoretically disregards the need to continue using brushes or sponges for hand asepsis.

  10. Developing a collaborative community partnership program in medical asepsis with tattoo studios.

    Science.gov (United States)

    Bechtel, G A; Garrett, C; Grover, S

    1995-10-01

    The possibility of transmission of infectious agents during tattooing has become a legitimate issue of concern for health care providers. A collaborative educational program was developed by a county health department, College of Nursing, and tattoo artists to address issues of medical asepsis with the goal of producing a mechanism for certification of tattoo studios. The group's effort was enhanced by recognizing each other's value systems and by the mutual need for a successful program. A framework for developing, implementing, and evaluating community partnerships was addressed. This program demonstrated that community health nurses can play an instrumental role in collaborating with both health care providers and personal-service workers to minimize transmission of infectious agents during cosmetic procedures.

  11. 浅谈外科无菌技术的教学%Experience in teaching surgical asepsis

    Institute of Scientific and Technical Information of China (English)

    文灿

    2004-01-01

    外科无菌技术(surgical asepsis)是通过清洁、消毒和灭菌等综合技术与措施,使手术环境和手术区域的病原微生物尽量减少到最低限度,尽可能防止发生手术污染、感染。

  12. Technology development and application of asepsis cool-filling desserts%无菌冷罐装含乳甜点的技术开发和应用

    Institute of Scientific and Technical Information of China (English)

    胡姝敏; 朱雅玲

    2013-01-01

    Abstracts:The technology development and application of asepsis cool-filling desserts that fit for China were studied.By studying technical characters of asepsis cool-filling desserts and relative hydrocolloids application character,the technology (parameter),technology flow and hydrocolloids were confirmed,and the hydrocolloids were composed for functional system applied for asepsis cool-filling desserts to produce desserts that have very good texture and sensory organ property,including neutrosphere chocolate dessert and acidic pudding dessert.%研究了适合我国现有生产条件和市场发展状况的无菌冷灌装甜点的技术与应用.通过研究无菌冷灌装甜点的工艺特征及相关胶体应用特征,选择适合的亲水胶体,通过科学实验设计和生产验证,完成了利用超高温灭菌和无菌罐装技术生产不同质构的含乳甜点的工艺流程和稳定体系的设计.

  13. 五年制无菌术模拟教学%The Five Year System Asepsis Simulated Instruction

    Institute of Scientific and Technical Information of China (English)

    郭晨明; 付明刚; 郭丽英; 吴楠; 毕晓娟

    2016-01-01

    五年制教学作为医学院校教学主体,在医学院校教学中具有重要地位,为进一步加强五年制教学水平,提高五年制外科无菌操作观念,将五年制教学作为外科学教学重中之重。外科无菌操作以无菌术为基本原则,是外科重要理论基础,为培养五年制学生实践中无菌观念建立无菌操作技能具有重要意义。阐述了在五年制无菌术模拟教学过程中的经验体会,旨在提高五年制无菌观念和如何无菌操作为临床实践做好基础教学。%Five year system teaching as the main body, medical colleges and universities teaching in medical colleges and universities has the important status in the teaching, in order to further strengthen the five year system teaching level, improve the five year system surgical aseptic operation concept, will the five year system teaching as a top priority of surgery teaching. Surgical aseptic operations to aseptic technique as the basic principle, is an important theoretical basis for surgery, to cultivate the five year system students practice aseptic concept establish aseptic operation skills is of the great significance. The five year system asepsis is discussed in this paper simulate the experiences in the process of teaching, aimed at improving the five year system sterile concepts and how to aseptic operation basis for the clinical practice teaching.

  14. Environmental surface asepsis.

    Science.gov (United States)

    Palenik, Charles John

    2005-09-01

    Environmental surface disinfection is easily accomplished with precleaning and disinfection techniques, and prepared surfaces or difficult-to-clean items can be covered with impermeable barriers. When carried out effectively, both practitioners and patients are protected from exposure to microorganisms that transmit disease and cause illness.

  15. HOMOIMPLANTE ORTOTÓPICO DE TENDÃO CALCÂNEO EM CÃES: CONSERVAÇÃO, ASSEPSIA E IMPLANTAÇÃO ORTHOTOPIC HOMOLOGOUS COMMON CALCANEOUS TENDON GRAFT IN DOGS: CONSERVATION, ASEPSIS AND IMPLANTATION

    Directory of Open Access Journals (Sweden)

    Alceu Gaspar Raiser

    2001-02-01

    Full Text Available Foi elaborado um modelo experimental de conservação, assepsia e implantação de tendão homólogo ortotópico, para avaliar a resposta orgânica, em 48 cães. Os implantes foram conservados em glicerina a 98%, durante 45 dias a seis meses e, após, reidratados e submetidos à ação anti-séptica do iodo durante 24h antes da implantação. A avaliação clínica e histopatológica após 11, 22, 44 e 132 dias de evolução não evidenciou qualquer sinal de infecção ou rejeição. Tendões homólogos ortotópicos conservados em glicerina a 98% estão indicados para corrigir perdas tendíneas.An experimental model of conservation, asepsis and implantation of homologous glycerine 98%-stored tendon graft is presented in order to evaluate the tissue reaction to implantation in 48 dogs. Grafts were preverved in sterile glycerin from 45 days to six months period. Before implantation grafts were placed in isotônic saline with iodine, during 24h period for rehydration and asepsis. Clinical evaluation and histopathologic analisis of biopsies were made at 11st , 22nd, 44th and 132nd post-operative day. There was not evidence of infection or graft rejection. Orthotopic homologous glycerine-stored tendon graft are indicated in reconstructive tendon surgery.

  16. Evaluation of asepsis, agar concentration and culture medium composition methods for in vitro propagation of Pimpinella anisum (Linn.) – Apiaceae
    Avaliação de métodos de esterilização, concentração de ágar e composição de meio de cultura para propagação in vitro de Pimpinella anisum (Linn.) – Apiaceae

    OpenAIRE

    Gabriela Tambosi; Gladys Daniela Rogge-Renner

    2010-01-01

    Pimpinella anisum, is a plant species popularly used as treatment for different diseases, although no studies exist which could prove its pharmacological activity. This species has no known methods of asepsis and adequate conditions to conduct in vitro propagation trials. To assess the most efficient method of seed sterilization and the most favorable concentration of agar and MS salts, five tests of asepsis and tests to evaluate the dry mass increase of germinated seed in 3g/L and 6g/L of ag...

  17. 茶树无菌播种建立植株再生体系%Establishment of Plant Regeneration System of Tea Trees by Asepsis Sowing

    Institute of Scientific and Technical Information of China (English)

    黄燕芬; 吴曦; 周国兰; 赵华富

    2013-01-01

    为提高茶树的繁殖速率,用近成熟的茶树种子以无菌播种方式获得试管苗,再以其茎段为外植体,建立了茶树高效植株再生体系.结果表明,剥除内外种皮的茶树种子发芽率为90%,较保留种皮的完全种子增加了13个百分点;茶树种子胚龄与发芽势、发芽率呈正相关,与萌发时间呈负相关;诱导茶树种子萌发适宜培养基为MS+6-BA 3.0 mg/L+IBA 2.0 mg/L+YE 200 mg/L或改良ER+6-BA 2.5 mg/L+NAA 0.2 mg/L;在改良ER+6-BA 1.5 mg/L+NAA 0.2 mg/L继代增殖培养基中培养,带芽组织切块的适宜增殖周期为39 d,增殖倍数5.4;壮苗、诱导根形成的适宜培养基为1/2改良ER+IBA 0.10 mg/L,发根率达80%.%To improve the reproduction rate of tea trees,using the stem of plantlet as explant,which was obtained by asepsis sowing with the nearly mature tea seeds,the optimum regeneration system of tea trees was established.The experimental results showed that the germination rate of tea seeds whose endotesta and exopleura were stripped out was 90%,increased by 13 percentage points compared with the whole seeds.The embryo age of tea seeds had positive correlation with germination energy and germination percentage,but was negatively related to the germination time.To induce tea seed germination,the optimum medium was composed of MS+6-BA 3.0 mg/L+IBA 2.0 mg/L+YE 200 mg/L or ER+6-BA 2.5 mg/L+NAA 0.2 mg/L.The tissue slicing of bud indicated that the suitable proliferation cycle was 39 days with proliferation multiple of 5.4 in the modified subculture medium (ER+6-BA 1.5 mg/L+NAA 0.2 mg/L).In addition,the optimum medium for making seedling strong and inducing root formation was modified 1/2 ER+IBA 0.10 mg/L,leading to 80 % rooting rate.

  18. 实验动物设施消毒与灭菌的化学药剂选择%Selection of Chemical Reagent for Asepsis and Steriolizations of Laboratory Animal Facilities

    Institute of Scientific and Technical Information of China (English)

    温泽锋; 郑振辉

    2011-01-01

    ln order to achive the reliable asepsis and sterilization in laboratory animal facilities, the selection of chemical reagents should base on the chemical characteristics of the reagent, aseptic methods and features of laboratory animal facilities. Chlorine dioxide is recommended because its utility does nol harm to the environment.%通过对实验动物设施消毒灭菌常用化学药剂化学性质及其在使用过程中综合因素的比较分析,为正确选择适合的消毒灭菌试剂和方法提供科学依据,同时着重介绍了二氧化氯作为一种绿色环保的化学药剂在动物设施消毒灭菌中的优势,建议在实验动物设施的消毒灭菌中加以推广应用.

  19. 外科无菌术的教学过程应与临床实践紧密结合%The Classroom Teaching of Surgical Asepsis Should be Closely Combined With Clinical Practice

    Institute of Scientific and Technical Information of China (English)

    张小丽; 李非

    2016-01-01

    Antiseptic and aseptic techniques are to prevent surgical infection and increase the safety and breadth of surgery. Surgical asepsis high requirements on the clinical teaching of operation room environment, and rules and regulations and management rules are dififcult to implement and execute in the teaching process of pure. In order to strengthen the aseptic concept, classroom teaching should be closely combined with clinical practice. The teacher repeatedly stressed that the principles of aseptic technique, and in a timely manner in violation of the principle of aseptic problems were corrected, and strict assessment, help students to establish the concept of sterile.%外科无菌术是使各种手术和操作保持无菌和防止感染的技术。外科无菌术对临床教学环境要求高,而且手术室规章制度和管理规则很难在单纯教学过程中贯彻执行。为强化无菌观念,课堂教学应该与临床实践紧密结合,老师要反复强调无菌操作的原则,及时对违反无菌原则的问题进行矫正,并进行严格考核,帮助学生树立扎实的无菌观念。

  20. 高效模式下医院手术室一次性无菌物品的管理%Under Highly Effective Revolution Pattern Hospital Operating Room Disposable Asepsis Goods Management

    Institute of Scientific and Technical Information of China (English)

    吴巧琴; 沈惠青

    2014-01-01

    Objective Discusses this courtyard operating room to the disposable asepsis goods management ef ect and the experience.Methods 2012January January through the application of consumables management team re integration, environment layout improvement, goods supply process restructuring and management tools to regulate the new, improved management of disposable sterile items. Results Low seniority nurses can take the object time; leak charge can find the cor ect; reduce the cost of waste; reduce the nurse not on duty time; improve the staf on the disposable use satisfaction; improve the surgeon on nurses' job satisfaction. Conclusion The standardized management ef ectively ensure the safe use of disposable sterile items, make work more orderly, more ef icient, the medical relations more harmonious, but also ful y embodies the patient centered service tenet.%目的:探讨本院手术室对一次性无菌物品管理的效果及体会。方法2012年1月起通过对耗材管理团队的再整合、环境布局的改进、物品供应流程的重组及新的管理工具的应用来进行规范、改进一次性无菌物品的管理。结果缩短了低年资护士拿取物品的时间;漏收费能及时发现更正;减少了科室成本浪费;降低了巡回护士不在岗时间;提高了科室人员对一次性物品使用的满意度;提高了外科医生对护士工作的满意度(P<0.01)。结论规范化管理有效地保证了一次性无菌物品的安全使用,使工作更有序,效率更高,医护关系更融洽,也充分体现了以患者为中心的服务宗旨。

  1. 聚丙烯塑料输液瓶拉环盖内面无菌可靠性调查%The asepsis security of the interior covers of polypropylene plastic dropping bottle

    Institute of Scientific and Technical Information of China (English)

    徐雪芬

    2008-01-01

    目的 探讨聚丙烯甥料输液瓶拉环盖内面的无菌可靠性.方法 将聚丙烯塑料输液瓶随机分为2组:对照组42瓶用生理盐水棉签直接采集开启后的聚丙烯塑料输液瓶拉环盖内面样本,实验组42瓶分别用0.5%碘伏、75%乙醇棉签常规消毒开启后的聚丙烯甥料输液瓶拉环盖内面,待干燥后用生理盐水棉签采集样本.所取样本均由主管检验师接种于无菌普通琼脂营养平板.置于37℃恒温培养箱内培养,以观察细菌生长情况.结果 2组细菌生长差异有统计学意义,X2=46.791,P<0.01.结论 聚丙烯塑料输液瓶拉环盖内面必须严格执行常规消毒后方能应用于临床.%Objective To study the asepsis security of the interior cover of polypropylene plastic dropping bottle.Methods The whole samples were divided randomly into two groups,each group included 42 polypropylene plastic dropping bottles,Samples of the control group were collected from the interior covers of dropping bottles wiping with sterile normal saline cotton swabs.Samples of the experimental group were also collected from the interior covers wiping with sterile normal saline cotton swabs aher disinfected by 0.5%iodophors and 75%alcohol respectively.All samples were inoculated on the aseptic plain agar nutrition flat plate.then incubated in 37℃ incubaton to observe the growth of bacteria.Results There Was statistical significance between the two groups in the growth of bacteria,χ2=46.791,P<0.01.Conclusions The interior covers of polypropylene plastic dropping bottle should be disinfected strictly before they are used in clinic.

  2. Medical Asepsis, Research, and Continuing Education

    Science.gov (United States)

    Trussell, Patricia M.; Crow, Sue

    1977-01-01

    Emphasizes the need that continuing education programs for nurses in hospitals orient newly employed graduate nurses specifically to infection control measures as carried out in that institution and then to reinforce these learnings by regular planned programs. Points out ways that those responsible for inservice nursing education can facilitate…

  3. Evaluation of asepsis, agar concentration and culture medium composition methods for in vitro propagation of Pimpinella anisum (Linn. – Apiaceae Avaliação de métodos de esterilização, concentração de ágar e composição de meio de cultura para propagação in vitro de Pimpinella anisum (Linn. – Apiaceae

    Directory of Open Access Journals (Sweden)

    Gabriela Tambosi

    2010-12-01

    Full Text Available Pimpinella anisum, is a plant species popularly used as treatment for different diseases, although no studies exist which could prove its pharmacological activity. This species has no known methods of asepsis and adequate conditions to conduct in vitro propagation trials. To assess the most efficient method of seed sterilization and the most favorable concentration of agar and MS salts, five tests of asepsis and tests to evaluate the dry mass increase of germinated seed in 3g/L and 6g/L of agar and MS in concentrations of 0%, 25%, 50%, 75% and 100%, were carried out. The samples that were immersed in a concentration of 2% sodium hypochlorite for 20 minutes and then washed in distilled water produced 100% of sterile plantlets. The greatest increase in dry mass was obtained with 75% and 100% of MS salts. Furthermore, the different concentrations of agar did not influence the percentage of the plantlets dry mass. Pimpinella anisum é uma planta popularmente utilizada como medicinal para diversas enfermidades, embora não existam estudos que comprovem sua atividade farmacológica. A espécie não apresenta metodologias conhecidas de assepsia e condições adequadas para experimentos de propagação in vitro. Para avaliar a forma mais eficiente de esterilização de sementes da espécie e melhores concentrações de ágar e sais do meio MS, foram feitos cinco testes de assepsia, bem como testes de avaliação do aumento de massa seca a partir de sementes germinadas em ágar 3g/L e 6g/L e MS nas concentrações 0%, 25%, 50%, 75% e 100%. A imersão em solução hipoclorito de sódio 2% por 20 minutos e lavagem em água destilada promoveu 100% de plântulas estéreis. O maior aumento de massa seca foi obtido com concentração de sais do meio MS de 75% e 100%, e as diferentes concentrações de ágar não influenciaram a obtenção de maior percentual de massa de plântulas cultivadas.

  4. 外科无菌伤口与感染伤口的处理%Management of the asepsis wound and non-asepsis wound in surgery

    Institute of Scientific and Technical Information of China (English)

    胡建美

    2008-01-01

    外科伤口处理正确与否直接影响伤口的愈合和患者的治疗时间,更换敷料的目的是观察和处理伤口,发现并消除不利于伤口愈合的因素,增强和促进有利因素,使伤口早日愈合。文章浅谈不同伤口的特点和敷料更换的方法以及防止伤口感染的处理措施。

  5. Photodynamic Antimicrobial Chemotherapy for Root Canal System Asepsis: A Narrative Literature Review

    Directory of Open Access Journals (Sweden)

    P. Diogo

    2015-01-01

    Full Text Available Aim. The aim of this comprehensive literature review was to address the question: Does photodynamic therapy (PDT improve root canal disinfection through significant bacterial reduction in the root canal system? Methodology. A comprehensive narrative literature review was performed to compare PDT effect with sodium hypochlorite as the comparative classical irrigant. Two reviewers independently conducted literature searches using a combination of medical subject heading terms and key words to identify relevant studies comparing information found in 7 electronic databases from January 2000 to May 2015. A manual search was performed on bibliography of articles collected on electronic databases. Authors were contacted to ask for references of more research not detected on the prior electronic and manual searches. Results. The literature search provided 62 titles and abstracts, from which 29 studies were related directly to the search theme. Considering all publications, 14 (48% showed PDT to be more efficient in antimicrobial outcome than NaOCl (0.5–6% concentration used alone and 2 (7% revealed similar effects between them. Toluidine blue and methylene blue are the most used photosensitizers and most commonly laser has 660 nm of wavelength with a 400 nm diameter of intracanal fiber. Conclusions. PDT has been used without a well-defined protocol and still remains at an experimental stage waiting for further optimization. The level of evidence available in clinical studies to answer this question is low and at high risk of bias.

  6. Novel Asepsis Cold-Infusion Technique%最新无菌冷灌装技术

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    KRONES Group has developed a kind of new PET germfree filling technology. The germfree cold filling system taking this new technology as its core now is being applied by many users in Germany, Switzerland and China, among which is Huiyuan Group.

  7. Photodynamic Antimicrobial Chemotherapy for Root Canal System Asepsis: A Narrative Literature Review.

    Science.gov (United States)

    Diogo, P; Gonçalves, T; Palma, P; Santos, J M

    2015-01-01

    Aim. The aim of this comprehensive literature review was to address the question: Does photodynamic therapy (PDT) improve root canal disinfection through significant bacterial reduction in the root canal system? Methodology. A comprehensive narrative literature review was performed to compare PDT effect with sodium hypochlorite as the comparative classical irrigant. Two reviewers independently conducted literature searches using a combination of medical subject heading terms and key words to identify relevant studies comparing information found in 7 electronic databases from January 2000 to May 2015. A manual search was performed on bibliography of articles collected on electronic databases. Authors were contacted to ask for references of more research not detected on the prior electronic and manual searches. Results. The literature search provided 62 titles and abstracts, from which 29 studies were related directly to the search theme. Considering all publications, 14 (48%) showed PDT to be more efficient in antimicrobial outcome than NaOCl (0.5-6% concentration) used alone and 2 (7%) revealed similar effects between them. Toluidine blue and methylene blue are the most used photosensitizers and most commonly laser has 660 nm of wavelength with a 400 nm diameter of intracanal fiber. Conclusions. PDT has been used without a well-defined protocol and still remains at an experimental stage waiting for further optimization. The level of evidence available in clinical studies to answer this question is low and at high risk of bias.

  8. Asepsis Control of Iniection Hematoporphvrin%血卟啉注射液无菌控制

    Institute of Scientific and Technical Information of China (English)

    周健; 李中全

    2004-01-01

    血卟啉是用氯化血红素经过化学半合成而得的多种卟啉类的混合物,主要成分是血卟啉、卟啉聚合体、羟乙基-乙烯基次卟啉及少量原卟啉.血卟啉注射液就是血卟啉的无菌水溶液.文章通过对血卟啉注射液的工艺流程以及检验过程的监控和检验,确认控制血卟啉注射液无菌的步骤和要点.证明以无菌灌装方式生产的血卟啉注射液无菌质量符合国家标准要求,并确保患者用药安全.

  9. 无菌检测室的管理%Asepsis examination room management

    Institute of Scientific and Technical Information of China (English)

    王美珍

    2011-01-01

    无菌检查法是用于检查国家药品标准中要求无菌的药品、原料、辅料及其他品种是否无菌的检查方法,无菌检测室是按照药典有关规定设计、配置、维护,用于无菌检测的实验室,本文从无菌室的设计、设施设备配置及日常维护、使用、操作管理等方面提出要求,强化对无菌检测室的管理.

  10. Achieving asepsis of banana leaves for the management of toxic epidermal necrolysis

    Directory of Open Access Journals (Sweden)

    Srinivas C

    2006-01-01

    Full Text Available Background: Banana leaf is used in many centers in India during the care of patients with toxic epidermal necrolysis (TEN and other extensive blistering disorders. Sepsis is an important cause of death in TEN patients and use of banana leaf may be a source of such infection. Aims: We conducted this study to detect the bacterial flora of the banana leaf and to examine various methods of rendering the leaf aseptic. Methods: Five pieces of banana leaf, 2 x 2 cm in size, were cultured separately in blood agar as follows: One piece was heated over a flame and one was soaked in boiling water and one was autoclaved. Methylated spirit was applied over one piece and ignited. One piece was placed on the media, ′as is.′ The Petri dishes were incubated examined after 48 h. Results: All the pieces except the autoclaved specimen of the leaf grew coagulase-negative staphylococci (CONS when aseptic precautions were not maintained and aerobic spore bearers when all aseptic measures were subsequently instituted during the procedure. Conclusion: We recommend measures to prevent possible transmission of bacterial infection by the leaf. Autoclaved and aseptically handled banana leaves may be used to reduce chance of infection in the treatment of TEN.

  11. The white epidemic and the asepsis of refined earthenware in Belle Époque São Paulo.

    Science.gov (United States)

    Souza, Rafael de Abreu E

    2012-12-01

    The article examines Brazilian refined earthenwares known as faiança fina (fine faience) and relates ideas about its production to the contextual backdrop of hygienist discourses in the city of São Paulo in the early twentieth century. Based on an analysis of glaze components, moisture expansion processes, and technological aspects of the production of earthenware recovered from the Petybon archeological site, it is suggested that the establishment of factories and the production and consumption of white ceramics in the city of São Paulo were partially a consequence of the vigorous hygienization policies and modernity projects then advocated by São Paulo's elites. PMID:23295654

  12. Experience in teaching asepsis of operative surgery%外科无菌技术的教学体会

    Institute of Scientific and Technical Information of China (English)

    雷艳; 周小林; 兰阳军; 杨爱军; 文灿

    2012-01-01

    @@ 外科无菌技术是以预防手术伤口感染为目的,在手术、穿刺、注射、换药等操作过程中必须遵守的原则和技术方法,是保证手术成功的必要条件.无菌技术是外科手术基本技术的重要组成部分,也是手术学教学的重要内容之一.提高外科无菌技术的教学效果,对手术学教学具有重要意义.

  13. Details Insure Ferment System Asepsis%着眼细节,确保发酵系统无菌化

    Institute of Scientific and Technical Information of China (English)

    唐孝鹏

    2007-01-01

    在纯种的微生物发酵过程中,染菌不仅不利于产品的质量与安全,还会给企业带来很大的损失。本文从发酵工艺的设计、设备的制作、工艺操作及现场卫生等各方面对发酵系统无菌化进行了初步分析,并提出一些自己的见解。

  14. 外科无菌术及其新进展%Surgical asepsis and its new progress

    Institute of Scientific and Technical Information of China (English)

    沈世强

    2005-01-01

    微生物普遍存在于人体和周围环境中,而外科疾病常可导致营养不良、免疫功能紊乱及其他增加感染的因素,因此手术后伤口感染是外科常见的并发症。引起创口或手术区域感染的主要途径有空气感染和接触感染,尤以后者较多见。保证外科病人不受感染是贯穿围手术期处理的根本原则,感染的发病率和严重性与病人的抵抗力、医院环境的细菌状态以及所采取的外科无菌术有关。

  15. The white epidemic and the asepsis of refined earthenware in Belle Époque São Paulo.

    Science.gov (United States)

    Souza, Rafael de Abreu E

    2012-12-01

    The article examines Brazilian refined earthenwares known as faiança fina (fine faience) and relates ideas about its production to the contextual backdrop of hygienist discourses in the city of São Paulo in the early twentieth century. Based on an analysis of glaze components, moisture expansion processes, and technological aspects of the production of earthenware recovered from the Petybon archeological site, it is suggested that the establishment of factories and the production and consumption of white ceramics in the city of São Paulo were partially a consequence of the vigorous hygienization policies and modernity projects then advocated by São Paulo's elites.

  16. 新型无菌病房的建设(2)%Build of a New-style Asepsis Ward(2)

    Institute of Scientific and Technical Information of China (English)

    孙鲁春; 龚伟; 邢玉斌; 杨彩青; 陈凤娜; 杨旭东; 贾博军; 余力

    2009-01-01

    目的 新型无菌病房现场实测以及通过模拟实验检测值与模拟(CFD)预测的结果比较.方法 采用各项仪器检测及利用计算液体力学(CFD)模拟分析.结果 经过各项指标参数对比及计算,均达到设计指标要求,新型无菌病房改善了空气质量,为患者提供了舒适安全的治疗环境,将有效降低医院感染率.结论 无菌病房在控制外源性污染物的同时,对于患者内源性感染起到有效控制作用.

  17. Bacilli- eliminated Filtration and Asepsis Packing of Beer%啤酒除菌过滤与无菌包装

    Institute of Scientific and Technical Information of China (English)

    王青山; 胡宜春

    2000-01-01

    描述了在啤酒除菌过滤和无菌包装系统上取得的实质性进展.导致过滤性能低下的主要因素在于存在胶质碳水化合物及在单通道过滤过程中使用了劣质硅藻土.通过在糖化锅中加β-葡聚糖酶并对单通道硅藻土过滤机过滤进行优化后得到的清酒,经高性能预过滤器及0.45μm的Millipore膜过滤器组合过滤,然后在包装中严格按质量保证程序操作,则微生物稳定性达到要求.除菌过滤与无菌包装能否成功在于好的"质量控制"程序.

  18. Verification of Method of Shuanghuanglian Injection Asepsis Test%双黄连注射液无菌检查的方法验证

    Institute of Scientific and Technical Information of China (English)

    赵亚丽; 吴英

    2011-01-01

    目的:主要是建立一种适合于双黄连注射液进行无菌检查的方法.方法:薄膜过滤法与直接接种法.结果:可照薄膜过滤检查法的检查条件进行双黄连注射液的无菌检查.

  19. Establishment on Asepsis Tissue Culture System of Actinidia hemsleyana%长叶猕猴桃初始无菌组培体系建立

    Institute of Scientific and Technical Information of China (English)

    蔡冬元

    2014-01-01

    [目的]建立长叶猕猴桃初始无菌培养体系.[方法]以长叶猕猴桃的带芽茎段、叶柄和叶片为外植体,以MS为基本培养基,展开三次双因子重复试验.[结果]长叶猕猴桃带芽茎段在MS+6-BA 1.00 mg/L+NAA 0.10 mg/L培养基中初始培养效果好;离体叶片小块在MS+6-BA 0.50 mg/L+NAA 0.10 mg/L培养基中初始培养效果好.[结论]为长叶猕猴桃组培苗的生产和工厂化快速繁育种苗奠定了基础.

  20. Asepsis Sowing and in vitro Propagation of Neofinetia richardsiana Christenson%短距风兰的无菌播种和快速繁殖

    Institute of Scientific and Technical Information of China (English)

    李靖嫄; 张小燕; 孔令杰; 彭德镇; 杨柏云

    2010-01-01

    @@ 1 植物名称短距风兰(Neofinetia richardsiana Christenson). 2 材料类别成熟未开裂蒴果中的种子. 3培养条件(1)种子萌发培养基:1/2B_5+6-BA 1.0mg·L~(-1)(单位下同)+NAA 0.2;(2)原球茎增殖培养基:B5+6-BA 1.O+NAA 1.5+0.1 % AC;(3)分化成苗培养基:B_5+6-BA 1.0+NAA 0.2+0.05% AC;(4)壮苗和生根培养基:1/2MS+IBA 0.5+NAA 0.3+0.03% AC.

  1. Porous Dressings Asepsis Check Method Validation Testing%多孔敷料的无菌检查方法验证试验

    Institute of Scientific and Technical Information of China (English)

    李强

    2010-01-01

    按照2005版二部要求,在进行常规的无菌检查之前,必须验证在实际检验条件下,所采用的检验方法是否适合于样品的无菌检查.本方案是关于多孔敷料的无菌检查--直接接种法的验证.

  2. Asepsis Sowing and in vitro Propagation of Brassolaeliocattleya Sung Ya Green%卡特兰无菌播种与快速繁殖

    Institute of Scientific and Technical Information of China (English)

    郑宝强; 王雁; 彭镇华

    2010-01-01

    @@ 1 植物名称 卡特兰杂交种(Brassolaeliocattleya Sung Ya Green'Green World'). 2材料类别 人工自花授粉后90、120、150、180和210 d的种子. 3培养条件 种子萌发培养基:(1)KC;(2)MS;(3)1/2MS.原球茎诱导、增殖和分化培养基:(4)1/2MS+NAA 0.2 mg·L-1(单位下同);(5)1/2MS+NAA 0.4;(6)1/2MS+6-BA 0.5+NAA 0.2;(7)1/2MS+6-BA 1.0+NAA0.2.

  3. Dental instrument asepsis: a survey of dental practice in Beijing%北京市各级医院口腔器械消毒方式调查

    Institute of Scientific and Technical Information of China (English)

    戴青; 邓小虹; 韩永成; 孙正

    2007-01-01

    目的 通过2次问卷调查评价北京市各级医院常用口腔器械消毒方法的情况,并针对存在问题给予规范.方法 2002、2005年对北京市各级医院常用口腔器械消毒方式进行问卷调查,将填写内容进行统计学处分析.结果 2005年北京市各级医院口腔诊疗器械存在多种消毒方式,其中采用压力蒸汽灭菌的比例提高,采用消毒剂浸泡、擦拭及其他方法处理口腔器械的比例下降.结论 2005年各级医院口腔器械消毒方式较2002年更为符合要求,但一、二级医院有待进一步提高.

  4. 白及的无菌播种和组织培养研究%Asepsis Sowing and Tissue Culture of Bletilla striata

    Institute of Scientific and Technical Information of China (English)

    曾宋君; 黄向力; 陈之林; 陈建通; 段俊

    2004-01-01

    本文报道了白及的无菌播种及试管苗茎尖组织培养实验.结果表明:种子在基本成熟时胚的萌发率和成苗率最高,萌发期与成苗期最短;胚萌发的最适培养基为1/2MS;在培养基中加入10%的椰子汁能提高萌发率与成苗率,加入1%的活性炭有利于试管苗的生长.试管苗茎尖在MS培养基附加6-BA 0.5mg/L和NAA 0.2 mg/L时增殖效果好.生根培养以1/2MS培养基附加NAA 0.5 mg/L效果最好,加上10%的香蕉汁有利于生根壮苗.

  5. The application of asepsis in breast tumor resectomy%乳腺肿瘤切除术中无菌术的应用

    Institute of Scientific and Technical Information of China (English)

    安梦滢; 热依汗古丽·力提甫

    2010-01-01

    目的 探讨无菌操作规程在乳腺肿瘤切除术中各个环节的实际应用以及对防止术后感染所起到的作用.方法 收集我院2007~2009年间实施乳腺肿瘤切除术的3 181例病例,从手术区域皮肤消毒的方法及范围、特殊无菌洞单及手术野无菌单的铺设、局麻药的抽取方法、手术人员无菌技术操作执行程度等进行阐述和分析.结果 严格执行无菌操作规程,使乳腺肿瘤切除后的无感染率达99.97%(3 180/3 181).结论 在乳腺肿瘤切除术中采用规范化的无菌操作法,对预防术后感染确有效果.

  6. [Clause Pouteau (1725-1775), surgeon at the l'Hôtel-Dieu de Lyon: his "asepsis" using water, fire and ligne propre].

    Science.gov (United States)

    Fischer, L; Touil, K

    1998-01-01

    Claude Pouteau, Hôtel-Dieu de Lyon's surgeon (1725-1775), did not improve surgical teaching, as Mareschal or Lapeyronie did with their reforming law. But he is reminiscent of an extremely skilful surgeon, always having a remarkable high rate of recoveries. For instance when it came to operate on bladder with a perineal approach (vesical cut), only three patients died out of one hundred and twenty operations. One century before Semmelweiss and more earlier than Pasteur, Pouteau thought that hospital-gangrene was not only caused by air miasma but also by direct contact, which could be indebted dirty instruments or hands, or hospital-made bandages. So he advised impeccably cleanliness for surgical students. According to his mind, soap was inadequate for cleaning hospital linen. Those must be pull out of neat material fitted by clean hands out of hospitals. It shall be supplied every day and never gathered inside. In order to keep clear of gangrene, the patient will not wait too long inside hospital. In case of bleeding, cautery must preferable to ligature for Pouteau "We can do without the bitter sadness of seeing a lighter wound become a lethal or incurable one ..." (Posthumous works, vol. III, p. 237-238).

  7. Research on Ventilation Strategy in the Bathroom of a Laminar Asepsis Ward%层流无菌病房卫生间通风方案研究

    Institute of Scientific and Technical Information of China (English)

    杨彩青; 杨旭东; 孙鲁春; 龚伟

    2009-01-01

    本文首先采用计算流体力学(CFD)模拟方法对某层流无菌病房3种不同卫生间送风方案进行了对比分析,分别比较了污染源在马桶和地板时室内污染物浓度的分布情况和关注区域的平均浓度情况,通过对比选出了适用于层流无菌病房内卫生间的最佳通风方案.其次,对房间内的风速和污染物浓度进行了测试,模拟结果与实测结果吻合很好,验证了该模拟模型的准确性.

  8. Asepsis Sowing and Tissue Culture of Dendrobium devonianum Paxt.%齿瓣石斛的无菌播种和组织培养

    Institute of Scientific and Technical Information of China (English)

    孙永玉; 李恒安; 闫红; 李昆

    2009-01-01

    @@ 1 植物名称齿瓣石斛(Dendrobium devonianum Paxt.),又名香棍草、大黄草. 2 材料类别成熟种子. 3 培养条件无菌播种培养基:(1)MS;(2)1/2MS;(3)N6;(4)White;(5)KC(Knudson C培养基);(6)VW(Vacin-Went培养基).分化成苗培养基:(7)N6+6-BA 0.5 mg·L~(-1)(单位下同);(8)N6+6-BA 1.0;(9)1/2N6+6-BA 0.5;(10)1/2N6+6-BA 1.0.生根壮苗培养基:(11)N6+NAA 0.1;(12)N6+NAA 0.5;(13)1/2N6+NAA 0.1;(14)1/2N6+NAA 0.5.

  9. 手术学教学中无菌观念的培养%Training for concept of asepsis in operation teaching

    Institute of Scientific and Technical Information of China (English)

    罗亚桐

    2010-01-01

    @@ 外科手术学是外科学的重要组成部分,是基础医学和临床医学的桥梁课程.外科手术学实验课的教学重点是无菌技术和手术基本技能的训练,使学生掌握进入临床实习前所必须具备的手术无菌观念和手术操作基本技能[1].

  10. Study on the Airbacteria Contamiration of Asepsis Used%无菌物品使用中空气细菌污染的检测

    Institute of Scientific and Technical Information of China (English)

    林德健; 蔡肇丽

    2004-01-01

    目的观察在无菌室使用无菌物品过程中,无菌物品受空气细菌污染的情况.方法在无菌室中放置两组无菌物品,分暴露组和无菌巾覆盖组,分别在0,60,120,180min进行无菌检验和空气细菌总数测定.结果暴露组细菌阳性率31.25%,无菌巾覆盖细菌阳性率15.00%,两组差异有显著性(P<0.05),合并各个不同时间组总阳性率23.13%,不同时间阳性率差异有显著性(P<0.05).结论随着无菌物品暴露于空气时间的延长,受细菌污染机会愈大,为控制院内感染提供了实验室依据.

  11. Asepsis Sowing and Rapid Propagation of Dendrobium pendulum Roxb.%肿节石解的无菌播种和快速繁殖

    Institute of Scientific and Technical Information of China (English)

    霍丽丽; 潘会堂; 张启翔

    2010-01-01

    @@ 1 植物名称肿节石解(Dendrobium pendulum Roxb.). 2 材料类别成熟种子. 3 培养条件以N6和MS为基本培养基.种子萌发培养基:(1)N6;(2)MS;(3)1/2MS;(4)VW(VacinWent培养基);(5)MS+椰乳100 mg·L~(-1)(单位下同).

  12. Asepsis Sowing and Rapid Propagation of Dendrobium moschatum(Buch.-Ham.)Sw.%杓唇石斛的种子培养与扩繁

    Institute of Scientific and Technical Information of China (English)

    黄卫昌; 殷丽青; 胡永红; 王新其

    2010-01-01

    @@ 1 植物名称构唇石斛[Dendrobium moschatum (Buck.-Ham.) Sw.]. 2 材料类别人工自花授粉后的成熟种子. 3 培养条件以MS、1/2MS为基本培养基.种子萌发培养基:(1) MSo;(2) MS+6-BA 0.5 mg·L-1(单位下同)+NAA 0.1;(3) MS+6-BA 2.0+NAA 0.2.原球茎增殖与分化培养基:(4) MS+6-BA 0.5+KT 0.5;(5)MS+6-BA 1.O+KT 0.5+NAA 0.2.壮苗与生根培养基:(6) 1/2MS+IBA 0.5.培养基(1)~(5)添加3.0%

  13. 二步灭菌法在植物组织培养中的应用%Application of Two-Step Asepsis in Plant Tissue Culture

    Institute of Scientific and Technical Information of China (English)

    杨宇; 金强; 王新建

    2010-01-01

    在马铃薯茎尖组织培养过程中,对外植体不同的灭菌方法进行研究.结果表明:用流水冲洗24 h,可以降低外植体的污染率和褐化率,同时对材料的生长无较大影响.升汞的灭菌效果虽然好于次氯酸钠,但接种后的效果较差.化学药剂的处理时间为5 min时,虽然污染率稍高,但其接种后效果相对较好.

  14. 无菌砖包图案偏差校正伺服控制%The Servo Control Of Asepsis Brick Package's Design-error Correction

    Institute of Scientific and Technical Information of China (English)

    马振江; 刘利

    2006-01-01

    本文主要介绍了一种基于条码快速识别的位置校正系统.详细介绍了电眼扫描两路条形码技术、角度编码器差补运算技术.并通过上述技术获得高精度的位置偏差,之后经过控制算法由伺服系统完成位置校正.最终实现了砖式牛奶包装机包装纸高速非匀速运动下精确定位,生产出形象佳、质量好的砖式牛奶包装.

  15. Comparative Study on Asepsis Technology for Outer Surface of Quail Eggshell%鹌鹑蛋壳外表面无菌化技术比较研究

    Institute of Scientific and Technical Information of China (English)

    宋东亮; 范伟杰; 索勋; 庞有志

    2009-01-01

    利用蛋用鹌鹑蛋对多种蛋外灭菌方法进行了比较,结果发现多种方法均能取得好的灭菌效果,而用瞬间沸水浸烫后立即在酒精中浸泡1 min,灭菌效果最好,对蛋内微生物和蛋的品质没有显著影响.

  16. The technology of phalaenopsis' asepsis sowing and rapid propagation%蝴蝶兰无菌播种快繁技术

    Institute of Scientific and Technical Information of China (English)

    廖福琴; 黄萍萍; 张永柏; 刘添锋

    2004-01-01

    蝴蝶兰无菌播种快繁技术的研究工作开始于2000年,经过3a的试验研究,掌握了蝴蝶兰种子组培快繁技术,包括种荚的采收适期、材料消毒、适宜的培养基配方、播种繁殖与壮苗生根以及试管苗移栽等方面,并成功繁育数万棵蝴蝶兰小苗,取得了一定的经济效益.

  17. Breaking Monopoly_New Domestic Asepsis Filling Machine%打破垄断的国产新型无菌包灌装机

    Institute of Scientific and Technical Information of China (English)

    正东

    2006-01-01

    长期以来,国外企业在无菌包及配套灌装机方面的绝对优势地位无人能够撼动,导致中国乳品市场由外国包装商控制和垄断。目前,瑞安市正东工控设备有限公司与北京富美包装材料有限公司研制推出了新一代、低成本、可环保的液态食品复合包材和符合中国包装行情的无菌灌装设备。

  18. Design of A Cool Machine for Barreled Fruit Juice of Asepsis%果浆无菌大桶装冷却设备的设计

    Institute of Scientific and Technical Information of China (English)

    张衍林; 朱松德; 贺红桦; 郭予南; 王光耀

    2000-01-01

    研究设计的果浆无菌大桶装冷却设备采用水作冷却介质,用偏心轮、连杆及摇杆组成平行四边形机构作为活动轨道,并与高低固定轨道配合,使大桶产生机械振动和左右摇动并向后移动,促进桶中心的热交换.解决了高温灭菌后充满率大于99.5%的大桶装果浆冷却十分困难的问题,保证了果浆的自然色泽和纯正口味,提高了产品质量.试验结果为1 h使大桶装桃浆从98℃降至40℃以下,达到了冷却目的.

  19. Application of HACCP during asepsis recovery milk production%HACCP在无菌包装复原奶生产中的应用

    Institute of Scientific and Technical Information of China (English)

    熊中平; 马纯燕

    2006-01-01

    探讨了危害分析与关键控制点(HACCP)在无菌包装复原奶产品中的应用,对无菌包装复原奶生产过程中的各个工序中可能造成的危害进行物理、化学、生物等方面的分析,确定出CCP点及其控制界限、检测体系、纠偏措施等,将生产过程中危害降到最低,有效保证产品质量和安全.

  20. 替硝唑注射液无菌检查方法的验证%Carrier on the confirmation of asepsis inspection method for tinidazole injection

    Institute of Scientific and Technical Information of China (English)

    范明

    2007-01-01

    目的 建立替硝唑注射液的无菌检查方法并对其进行验证.方法 采用2005年版二部附录无菌检查项下薄膜过滤法.结果 确定了替硝唑注射液无菌检查法的最佳冲洗量.结论 为替硝唑注射液无菌检查方法提供依据.

  1. Design and operation performance analysis of bathroom in asepsis ward%无菌病房卫生间设计及应用效果分析

    Institute of Scientific and Technical Information of China (English)

    孙鲁春; 龚伟; 杨彩青; 陈凤娜; 杨旭东

    2009-01-01

    介绍了该工程净化空调系统的组成、主要设计参数指标的优化选择和实验测试及数值模拟预测的结果.工程建成启用后采用多种检测方式对各项参数指标进行了跟踪检测及分析,系统运行良好,达到了预期的效果.

  2. Asepsis Sowing and in vitro Propagation of Hygrochilus parishii (Rchb.f.) Pfitz%湿唇兰无菌播种与快速繁殖技术

    Institute of Scientific and Technical Information of China (English)

    梁干君; 李星宇; 胡琦敏; 刘元

    2011-01-01

    1植物名称湿唇兰[ Hygrochilus parishii (Rchb.f.)Pfitz.].2材料类别湿唇兰的成熟种子.3培养条件(1)原球茎诱导培养基:MS+6-BA0.2mg· L-1+NAA0.5mg· L-1+蔗糖3%+琼脂0.7%,pH5.5;(2)原球茎的增殖培养基:MS+6-BA1.0 mg·L-1 +NAA 0.5 mg·L-1 +60g/L香蕉+蔗糖3%+琼脂0.7%,pH5.5;(3)原球茎的分化培养基:MS+6-BA 1.5 mg·L-1 +NAA0.2mg·L-1+ +60g/L香蕉+蔗糖3%+琼脂0.7%,pH5.5;(3)壮苗生根培养基:1/2MS+NAA 0.5 mg· L-1 +6-BA 0.1mg·L-1 +0.1%活性碳+蔗糖2%+琼脂0.7%,PH5.6培养温度为(25+2)℃,光照时间12 h·d-1,光强为40 umol·m-2·S-1.

  3. Asepsis Sowing and Rapid Propagation of Coelogyne fimbriata Lindl.%流苏贝母兰的无菌播种与快速繁殖

    Institute of Scientific and Technical Information of China (English)

    张小燕; 白茗洲; 彭德镇; 孔令杰; 杨柏云

    2009-01-01

    @@ 1 植物名称 流苏贝母兰(Coelogyne fimbriataLindl.). 2 材料类别 种子. 3 培养条件 (1)种子萌发培养基:1/2MS+6-BA 0.5mg·L-1(单位下同)+NAA 0.5+0.1%AC(活性炭);(2)原球茎增殖培养基:B5+6-BA 1.2+NAA 0.6+15 g·L-1蔗糖;(3)分化成苗培养基:B5+6-BA 2.0+NAA 0.2+0.1%AC;(4)壮苗生根培养基:1/2MS+NAA 0.5+0.1%AC.以上除培养基(2)外均附加40 g·L-1蔗糖,用7 g·L-1琼脂固化.所有培养基pH为5.6~5.8,培养温度为(25±2)℃,光照强度为30~40 μmol·m-2·s-1,光照时间为12 h·d-1,悬浮培养时振荡的转速为110r·min-1.

  4. Aminophylline injection Asepsis Check Test and Verify Test Methodology%氨茶碱注射液无菌检查试验及方法学验证试验

    Institute of Scientific and Technical Information of China (English)

    袁文婧; 仉晶

    2011-01-01

    目的:对氨茶碱注射液进行无菌检查试验和无菌方法学验证,确认无菌检查所采用的方法适合于该药品的无菌检查.方法:无菌检查法.结果:各实验菌均生长良好,供试品的该检验量在该检验条件下无抑菌作用或其抑菌作用可以忽略不计.结论:无菌检查的方法适用于氨茶碱注射液的无菌检查.

  5. 无菌分离和体外培养旋毛虫成虫方法的改进%Improved method of asepsis separation and culture in vitro of Trichinella spiralis adult

    Institute of Scientific and Technical Information of China (English)

    刘俊琴; 李科荣; 申丽洁

    2008-01-01

    目的 探索一种既简便又能用于大量收集和培养旋毛虫成虫的方法. 方法 在旋毛虫肌幼虫收集方法的基础上,结合现有方法和旋毛虫成虫的特点改进试验. 结果 10只成年大鼠,每只大鼠感染8 000条旋毛虫脱囊幼虫,收集到纯净成虫约44 600条,回收率55.75%.培养24 h后成虫活动活跃,并产生新生蚴,运动活泼. 结论 该法是一种既简便又能用于大量收集纯净的﹑活力较好的旋毛虫成虫的方法.

  6. The Effect of Delivering by Asepsis Supply Department to Control the Hospital Internal Infections%消毒供应室下送工作在控制院内感染中的作用

    Institute of Scientific and Technical Information of China (English)

    梁翠云

    2008-01-01

    目的:严格下送程序,使下送无菌物品不受到污染,达到控制院内感染、提高医院整体医疗质量的目的.方法:做好下送回收车的管理和下送护士手的卫生与分发无菌物品的程序,每周进行2次抽查进行细菌培养.结果:每周下送车内的细菌菌落数均≤5 cfu/cm2,符合无菌物品放置的标准要求;手的细菌菌落数在≤5 cfu/cm2,符合Ⅱ类区域工作人员手的要求.结论:严格要求下送车辆的清洁消毒、专车专用和下送工作人员的手卫生,有助于减少消毒物品受到污染的机会,防止交叉感染,可有效控制院内感染的发生.

  7. Application of asepsis-protective envelope in bowel decompression of intestinal obstruction surgery%无菌保护套在肠梗阻手术肠管减压中的运用

    Institute of Scientific and Technical Information of China (English)

    徐海燕

    2014-01-01

    目的:探讨一次性无菌保护套在肠梗阻手术肠管减压时对减少切口和手术区域的污染所起到的作用.方法:选择肠梗阻手术肠道减压使用一次性保护套的患者20例作为试验组,按照1:1比例选择同期手术,术中肠管减压使用常规方法的患者20例作为对照组.结果:试验组在手术时间、术中冲洗量、术中麻醉费用、术后3d病人体温各观察指标上均优于对照组(P<0.05).结论:无菌保护套在肠梗阻手术减压中能有效的减少对腹腔和切口的污染.

  8. TBA-9型利乐无菌包装机的条码识别系统的分析与改进%Reconstruction of the Bar Code Recognition System on TBA-9 Asepsis Packaging Machine

    Institute of Scientific and Technical Information of China (English)

    陈淼; 贾存良

    2006-01-01

    鉴于目前国内饮料包装技术还不发达,介绍了一种利用89C2051单片机对进口包装技术的改进方案.该方案在不改变原电路的情况下,串接入一自行设计的信号转换电路,使得重新设计的条码图案能够为原识别系统正确识别.

  9. Certification Tests of Sodium for Infection Asepsis Check Mean%注射用磷霉素钠的无菌检查法方法验证试验

    Institute of Scientific and Technical Information of China (English)

    李晓冰

    2007-01-01

    目的:确认所采用的方法适合于该药品的无菌检查.方法:薄膜过滤法.结果:经试验各实验菌能够正常生长.结论:经过验证,该方法能够有效地完成注射用磷霉素钠的无菌检查,适合该药品的无菌检查.

  10. 豆瓣兰的无菌播种与快速繁殖%Asepsis Sowing and in vitro Propagation of Cymbidum serratumr(Schltr)Y.S.Wu et S.C.Chen

    Institute of Scientific and Technical Information of China (English)

    卜朝阳; 何荆洲; 闭志强; 董伟清; 严华兵

    2010-01-01

    @@ 1 植物名称豆瓣兰[Cymbidum serratum(Schltr)Y.S.Wu et S.C.Chen]. 2 材料类别种子. 3 培养条件种子萌发培养基:(1)MS+1 mg·L~(-1)BAP+10%椰子水;(2)改良Kyoto+1 mg·(-1)BAP+10%椰子水;(3)1/3MS+1 mg·L~(-1)BAP+10%椰子水.

  11. 甲硝唑葡萄糖注射液注射液无菌检查方法的验证%The validation for the asepsis check method of the metronidazole glucose injection

    Institute of Scientific and Technical Information of China (English)

    栾东晓; 张彩霞

    2010-01-01

    目的 确定甲硝唑葡萄糖注射液的无菌检查方法.方法 按中国药典2005年版二部无菌检查法(附录XI H)中薄膜过滤法进行验证.结果 采用薄膜过滤法细菌以500ml/筒冲洗量、真菌以500ml/筒冲洗量检查,在规定条件下敏感菌可正常生长.结论 甲硝唑注射液的无菌检查可采用薄膜过滤法检查(500ml/筒冲洗量).

  12. Cause and management of epidural region infection after asepsis craniotomy%无菌开颅术后硬膜外区域感染的原因及治疗

    Institute of Scientific and Technical Information of China (English)

    田道锋; 陈谦学; 刘仁忠; 陈治标; 蔡强; 王军民

    2006-01-01

    无菌开颅术后硬膜外区域感染包括头皮感染、骨瓣骨炎或硬膜外积脓等,目前硬膜外区域感染的原因尚不清楚,各个医院的治疗方法不尽相同,作者总结10年来23例无菌开颅术后硬膜外区域感染患者的病例资料,并对其原因和治疗进行探讨。

  13. 护理专业无菌操作技术实验教学方法的改进%To Improve the Experiment Teaching Method of Asepsis in Nursing Speciality

    Institute of Scientific and Technical Information of China (English)

    黄银欢; 许传霞

    2011-01-01

    目的 改进护理专业无菌操作技术实验教学方法.方法 对本校护理专业无菌操作技术实验进行临床模拟教学改进.结果 增强了护理专业学生对无菌概念的理解,提高学生的综合能力.结论 临床模拟教学法可作为一项行之有效的方法,在护理专业无菌操作技术实验教学中推广.

  14. 蝴蝶兰种子无菌播种中诱导增殖原球茎试验研究%Study on Propagation of Protocorm of Phalenopsis by Induction in Asepsis Sowing

    Institute of Scientific and Technical Information of China (English)

    余慧琳; 胡月华; 朱一仪

    2009-01-01

    利用不同激素浓度及添加物对蝴蝶兰未成熟胚有效原球茎进行诱导研究.结果表明:进行无菌播种采收未开裂的果荚比已开裂的要有利的多,采摘最佳时间为授粉后120 d;原球茎增殖效果最佳的激素组合是BA 3.0+NAA 0.2;200 mL/L椰乳对蝴蝶兰PLB增殖的影响效果最好,其次为香蕉和马铃薯.

  15. The research on Cephalosporins miner sodium for injection asepsis check to eliminate antibacterial property%头孢米诺钠无菌检查消除抑菌性的研究

    Institute of Scientific and Technical Information of China (English)

    王洪珊; 岳志萍

    2010-01-01

    目的:依据中国药典2010年版无菌检查法的相关规定,通过一系列试验,建立头孢米诺钠无菌检查法.方法:用规定的各种试验菌,并采用β-内酰胺酶消除抑菌性进行试验.结果:头孢米诺钠采用薄膜过滤法进行无菌检查,对大肠埃希菌有较强的抑菌活性.结论:头孢米诺钠无菌检查需加入β-内酰胺酶来消除抑菌活性,以保证其无菌检查的准确性.

  16. 苯甲酸钠在组培中对外植体污染及分化的影响%Preliminary studies on effect of sodium benzoate to the system of asepsis explants

    Institute of Scientific and Technical Information of China (English)

    蔡建荣; 黄萍萍

    2008-01-01

    以"林畲早薯"山药茎段、"龙薯3号"甘薯茎尖和"六月红"芋茎尖为外植体,研究了培养基中添加高温处理和常温无菌过滤处理25 mg/L苯甲酸钠的杀菌抑菌效果.结果表明,25 mg/L苯甲酸钠能有效降低植物组织培养过程中的污染率,并且对外植体分化率的影响不明显;经高温处理和常温下过滤处理的苯甲酸钠在植物组织培养中的杀菌抑茵效果区别不显著.

  17. Some Methods for Preventing the Infection in Hospital by Asepsis Supply Department%消毒供应室预防医院感染的相关措施

    Institute of Scientific and Technical Information of China (English)

    张洪毅

    2006-01-01

    目的:通过加强质量管理,严格物品流通各个环节的操作规程,消除交叉感染的隐患,达到控制院内感染,提高医院整体医疗质量.方法:通过质量管理标准对供应室可能产生感染隐患的建筑布局和工作流程进行分析改造.结果:改进后的供应室工作流程使医院的感染发生率下降为零.结论:建立及完善供应室医院感染管理制度和合理的布局,改进消毒和物品流通的流程,是供应室控制医院感染的重要环节.

  18. The research on Cephalosporins XiDing sodium for injection asepsis check eliminate antibacterial property%头孢西丁钠无菌检查消除抑菌性的研究

    Institute of Scientific and Technical Information of China (English)

    邹晓平; 张东霞

    2011-01-01

    目的:依据中国药典2010年版无菌检查法的相关规定,通过一系列试验,建立头孢西丁钠无菌检查法.方法:用规定各种试验菌,同时使用β-内酰胺酶进行试验.结果:头孢西丁钠采用薄膜过滤法进行无菌检查,对大肠埃希菌有较强的抑菌活性.结论:头孢西丁钠无菌检查需加入β-内酰胺酶来消除抑菌活性,以保证其无菌检查的准确性.

  19. Study on Packing and Cost Benefit of Asepsis Appliance in Disinfection Department%供应室无菌物品包装与成本的效益研究

    Institute of Scientific and Technical Information of China (English)

    黄小虹; 候建民

    2005-01-01

    目的减少消毒费用,降低医疗成本,提高工作效率.方法更换传统的用棉布作为包装材料的方式,采用多种颜色一次性使用的无纺布巾作为各种治疗包的包装材料,取消了用贮槽盛装棉球、纱块,改用环氧乙烷小包装.将其费用支出与干预措施前相比较.结果消毒费用减少,耗材支出减少.结论降低了消毒费用成本,激励了员工的积极性,提高了工作效率,从而增加了经济效益.

  20. 手术室干备无菌持物钳及盛器更换时间的探讨%A discuss about the time to change dry asepsis frame and vessel in operation room

    Institute of Scientific and Technical Information of China (English)

    王伟; 单雪梅; 徐雪松

    2006-01-01

    目的:探讨手术室环境中干备无菌持物钳及其盛器合适的更换时间.方法:选择5个手术间的5个干备无菌持物钳.结果:打开后未使用前做为对照组和使用后4h、8h无细菌生长,12h和24h阳性样本数分别为17(28.33%)和50(83.33%).结论:手术室环境中干备无菌持物钳及其盛器的更新时间以8h为宜.

  1. The Effect of Delivering by Asepsis Supply Department to Control the Hospital Internal Infections%消毒供应室下送工作在控制院内感染中的作用

    Institute of Scientific and Technical Information of China (English)

    原丽萍; 张海燕; 谢朱红

    2006-01-01

    目的:严格下送下收程序,使下送无菌物品不受到污染,达到控制院内感染,减少交叉感染,从而提高医院整体医疗质量.方法:做好下送、回收车的管理,清洁消毒,专车专用和下送护士手的清洁与分发无菌物品的程序,每周进行二次抽查进行细菌培养.结果:每周下送车内的细菌菌落数均≤5 cfu/cm3,符合无菌物品放置的标准要求;手的细菌菌落数在≤5 cfu/cm3,符合II类区域工作人员手的要求.结论:严格要求下送车辆的清洁消毒、专车专用和下送工作人员手的清洁,有助于减少消毒物品受到污染的机会,防止交叉感染,可有效控制医院内的感染发生.

  2. International Certification Requirements for Simulating Validation of Asepsis Process for Raw Material Drugs%原料药无菌工艺模拟验证国际认证要求

    Institute of Scientific and Technical Information of China (English)

    高海燕; 陈军丽; 丁恩峰

    2011-01-01

    在欧关药政法规体系里面,如果无菌制剂采用无茵原料药(API)来制备,那么,无菌原料药的无菌性质对于制剂来说是至关重要的.无菌原料药一般采用两种方法来制遣灭菌工艺和无菌工艺.当原料药采用无菌工艺制造时,因为这种工艺无菌保证水平(SAL)较低,因此,需要采用模拟验证来评估无菌工艺的保证能力.在目前制药行业,通常采用无菌工艺模拟验证(也称为培养基灌装)方式评估工艺无菌保证能力.

  3. 高原地区骨关节无菌术后切口感染51例分析%Analysis of infection of incisional wound after osteoarticular asepsis at high altitude : report of 51 cases

    Institute of Scientific and Technical Information of China (English)

    林秀来; 殷作明; 何娟

    2003-01-01

    目的:探讨高原地区骨关节无菌术后切口感染的发生原因、处理方法及效果.方法:对51例高原髋关节无菌术后切口感染病例采用广谱抗生素、局部换药和伤口扩创、置管冲洗、引流等方法治疗.结果:治愈49例,无效2例.结论:针对感染后及早期诊断和正确处理是提高疗效的关键;同时外科医生应重视切口感染的预防,以减少切口感染的发生.

  4. 非PVC膜在不可最终灭菌的无菌粉原料药内包装上的应用%The application of non- PVC membrane on the inultimate asepsis pharmaceutical product powder

    Institute of Scientific and Technical Information of China (English)

    张庆武; 孙志昆; 陆超

    2005-01-01

    在剖析原有的不可最终灭菌的无菌粉原料药包装方式弊端的基础上,探索了采用非PVC多层共挤输液膜作为某种不可最终灭菌的无菌粉原料药的内包材,通过严格的质量认证,确证采用新的包装方式后药品质量稳定可靠,达到了预期效果.新的包装方式应用于生产中使药品的综合包装成本大幅度降低,排污量明显减少,提高了药品的安全性,具有显著的社会效益、环境效益和经济效益,具有推广价值.

  5. 空气监测在无菌贮存区中的临床意义研究%Study on the impact of air surveillance and monitoring in asepsis storage areas

    Institute of Scientific and Technical Information of China (English)

    陈健玲; 许红璐; 朱杏花; 梁金秀

    2002-01-01

    目的探讨影响无菌贮存区空气监测超标的因素.方法采用平板暴露沉降法,对无菌贮存区采取监控,并对措施前后 5个月的监测结果进行对比.结果采取措施前空气培养菌落数平均值是 282.6~409.6·cfu/cm3;采取措施后平均值是0~62.8·cfu/cm3,经统计学处理P值<0.001,采取措施前后差异有显著意义.结论只有加强无菌贮存区环境监控,不断完善环境监控条件,才可保证空气监测达标.

  6. The research on Cephalosporins XiDing sodium for injection asepsis check eliminate antibacterial property%头孢西丁钠无菌检查消除抑菌性的研究

    Institute of Scientific and Technical Information of China (English)

    邹晓平; 张东霞

    2011-01-01

    目的:依据中国药典2010年版无菌检查法的相关规定,通过一系列试验,建立头孢西丁钠无菌检查法.方法:用规定各种试验菌,同时使用β-内酰胺酶进行试验.结果:头孢西丁钠采用薄膜过滤法进行无菌检查,对大肠埃希茼有较强的抑菌活性.结论:头孢西丁钠无菌检查需加入β-内酰胺酶来消除抑菌活性,以保证其无菌检查的准确性.

  7. Emphasis on several concepts in teaching asepsis of operative surgery%外科手术学无菌术教学中应强化几个观念

    Institute of Scientific and Technical Information of China (English)

    刘安堂; 张盈帆; 沈宏亮; 王来根; 季华萍; 张爱芹; 杨波; 江华

    2011-01-01

    @@ 无菌术是临床医学的一个基本操作规范,外科的所有手术操作都是在无菌条件下进行,在操作过程中如不严格无菌操作,病原微生物就可能进入伤口,引起感染.培养学员掌握外科无菌技术,是外科手术学教学的核心内容和最重要的目标[1].

  8. Clinical relevance of surgical site infection as defined by the criteria of the Centers for Disease Control and Prevention

    DEFF Research Database (Denmark)

    Henriksen, N A; Meyhoff, C S; Wetterslev, J;

    2010-01-01

    (CRSSI) and whether there is agreement between evaluations according to the CDC criteria, the ASEPSIS score (Additional treatment, presence of Serous discharge, Erythema, Purulent exudate, Separation of the deep tissues, Isolation of bacteria and duration of Stay) and CRSSI. We included 54 patients....... There was substantial agreement between the CDC criteria and CRSSI [kappa=0.69; 95% confidence interval (CI): 0.55-0.83] and fair agreement between the ASEPSIS score and the CDC criteria (kappa=0.23; 95% CI: 0-0.49) and between the ASEPSIS score and CRSSI (kappa=0.39; 95% CI: 0.17-0.61). The CDC criteria represent...

  9. SUPPOSE TO ADMINISTRATE THE PERMUTATION OF DISINFECT AND ASEPSIS ARTICLES BY MEANS OF CLASSIFY CODING AND COMPUTER NETWORK%分类编码--电脑联网管理消毒及无菌物品交换之设想

    Institute of Scientific and Technical Information of China (English)

    曾桂珍; 吴慧堃; 袁小玲

    2005-01-01

    针对目前国内供应室无菌物品交换方法效率低的问题,提出分类编码-电脑联网管理消毒及无菌物品交换的设想,包括:①电脑联网及物品管理系统功能设计;②回收物品分类编码及编上顺号;③按分类编码放置,并设有编码及顺号登记卡;④无菌物品发放区根据电脑屏幕显示发放消毒及无菌物品,并在编码及顺号登记卡上写上日期、无菌物品去向,经手人签名等.避免少收、漏收、多收及丢失等现象,控制物品在收和送过程中的动态平衡,物品循环使用率增加,降低成本.

  10. Clinical relevance of surgical site infection as defined by the criteria of the Centers for Disease Control and Prevention

    DEFF Research Database (Denmark)

    Henriksen, N A; Meyhoff, C S; Wetterslev, J;

    2010-01-01

    (CRSSI) and whether there is agreement between evaluations according to the CDC criteria, the ASEPSIS score (Additional treatment, presence of Serous discharge, Erythema, Purulent exudate, Separation of the deep tissues, Isolation of bacteria and duration of Stay) and CRSSI. We included 54 patients....... There was substantial agreement between the CDC criteria and CRSSI [kappa=0.69; 95% confidence interval (CI): 0.55-0.83] and fair agreement between the ASEPSIS score and the CDC criteria (kappa=0.23; 95% CI: 0-0.49) and between the ASEPSIS score and CRSSI (kappa=0.39; 95% CI: 0.17-0.61). The CDC criteria represent...... a suitable standard definition for monitoring and identifying SSI, even if some cases of less clinically significant superficial SSI are included....

  11. Hand hygiene and aseptic technique in the emergency department.

    Science.gov (United States)

    Al-Damouk, M; Pudney, E; Bleetman, A

    2004-02-01

    Hand hygiene and simple aseptic measures before invasive procedures are effective in reducing rates of healthcare-associated infection. The perceived urgency of a clinical situation in the emergency department, however, may influence medical staff's compliance with good practice in infection control. The aims of this prospective, single-blinded, observational study were twofold. First, to assess doctors' compliance with good practice in hand hygiene between patient episodes and asepsis during invasive procedures in the emergency department. Second, to assess the effect of clinical urgency on compliance with good practice in hand hygiene and asepsis during invasive procedures. Good practice standards for asepsis in invasive procedures and hand hygiene between patient episodes were compiled from a literature search. Doctors' compliance with these standards was observed in two emergency departments (UK and New Zealand). Observed clinical cases were classified as immediate, urgent and non-urgent based on the triage system. There was poor compliance with good practice guidelines for asepsis in invasive procedures in both centres. Staff achieved high compliance with the guidelines in only 27% of cases in the UK and 58% of cases in New Zealand. Clinical urgency did not appear to adversely affect compliance with aseptic good practice. Hand hygiene between patient consultations was very low at 14% in the UK and 12% in New Zealand. Asepsis and hand hygiene was poor in both the UK and New Zealand emergency departments. There may be a need for some compromise in standards of asepsis in very sick patients due to the urgency of the clinical situation. Compliance in all situations especially non-urgent procedures needs to be improved.

  12. Instructional Analysis for Health Occupations.

    Science.gov (United States)

    East Texas State Univ., Commerce. Occupational Curriculum Lab.

    This instructional analysis centers on identifying the skills, related knowledge, teacher activities, and student activities that are central to teaching various topics included in the core curriculum for health occupations courses. Addressed in the volume are the following instructional areas: first aid; medical terminology; medical asepsis;…

  13. Health Occupations Curriculum. Skills and Theory for Health Assistant. Volume 2, Units 5-7.

    Science.gov (United States)

    Arizona State Dept. of Education, Phoenix.

    This volume consists of three units of a basic core curriculum that is intended for all health workers. Covered in the units are the following topics: (1) the body's need for food, the effect of food on the body, the five food groups, the six nutrients, and therapeutic diets; (2) general principles of asepsis, specific methods of maintaining…

  14. Reading Right: A Text for Reading, Volume 3. English for Special Purposes Series: Nursing Aide.

    Science.gov (United States)

    Hubbard, Katherine E.

    This is the third of four volumes devoted to reading instruction, in a series of materials for teaching English as a second language to adult nursing aide students. The three units included deal with fundamentals in nursing: asepsis, measurement and abbreviation, and emergencies. Each unit consists of readings interspersed with Cloze passages,…

  15. Relaparotomy: review of indications and outcome in tertiary care hospital

    Directory of Open Access Journals (Sweden)

    Prachi Srivastava

    2016-02-01

    Conclusions: Relaparotomy is a rare condition which surgeon might have to encounter. However, ensuring proper hemostasis and asepsis during surgical procedures can reduce the incidence of relaparotomy. Calculative decision before embarking on relaparotomy can decrease the incidence of morbidity and mortality associated with the procedure. [Int J Reprod Contracept Obstet Gynecol 2016; 5(2.000: 520-524

  16. Multiple Learning Strategies Project. Medical Assistant. [Regular Vocational. Vol. 1.

    Science.gov (United States)

    Varney, Beverly; And Others

    This instructional package, one of four designed for regular vocational students, focuses on the vocational area of medical assistant. Contained in this document are twenty-six learning modules organized into three units: language; receptioning; and asepsis. Each module includes these elements: a performance objective page telling what will be…

  17. A Comparison of In-service Teaching Methods on Clinical Aspesis for Dental Personnel.

    Science.gov (United States)

    Fotos, Pete G.; And Others

    1990-01-01

    Results of a study with dental assistants and hygienists, clinic clerks, supply and sterilization personnel, receptionists, and housekeeping and secretarial staff (n=80) suggest that inservice training programs on infection control and clinical asepsis procedures may be better accomplished by practical demonstration than by lecture or seminar.…

  18. Health Occupations Education I. Module No. I-A to I-G.

    Science.gov (United States)

    Dunmeyer, Kathryn; And Others

    This set of 7 modules on medical and surgical asepsis is 1 of 11 sets in the Health Occupations Education I instructional package for the first year of a 2-year course of study. The materials are designed to prepare students through individualized instruction for entry-level job opportunities on health care teams in a variety of practice settings.…

  19. Multiple Learning Strategies Project. Medical Assistant. Visually Impaired. [Vol. 1.

    Science.gov (United States)

    Varney, Beverly; And Others

    This instructional package, one of two designed for visually impaired students, focuses on the vocational area of medical assistant. Contained in this document are twelve learning modules organized into five units: language; receptioning; asepsis; supplies and equipment maintenance; and diagnostic tests. Each module, printed in block type,…

  20. A Comparison of Student-Centered versus Traditional Methods of Teaching Basic Nursing Skills in a Learning Laboratory.

    Science.gov (United States)

    Jeffries, Pamela R.; Rew, Sandy; Cramer, Joni M.

    2002-01-01

    To learn surgical asepsis content, 70 nursing students used a student-centered interactive lab and 50 used textbooks, videotapes, and lecture-demonstrations. There were no differences in cognitive gains or ability to demonstrate the skills learned. The student-centered group was significantly more satisfied with their learning. (SK)

  1. Units of Instruction. Health Occupations Education. Volume I. [Teacher's Guide].

    Science.gov (United States)

    East Texas State Univ., Commerce. Occupational Curriculum Lab.

    Ten units on health occupations are presented in this teacher's guide. The units are the following: recording vital signs; job application and interview; grooming and personal hygiene; health careers; medical careers; medical ethics; medical terminology and abbreviations; medical asepsis; basic patient care (e.g., measuring and recording fluid…

  2. Metabolic and oncological consequences of laparoscopic surgery

    NARCIS (Netherlands)

    N.D. Kannekens-Bouvy (Nicole)

    1997-01-01

    textabstractIn 1986, Philip Mouret and his colleagues performed the first laparoscopic cholecystectomy. They initiated the most revolutionary change in traditional surgery, since the introduction of anaesthesia, asepsis, antibiotics and blood-transfusion. At the same time, industry propelled this de

  3. 2003 CDC guidelines offer more choices for managing operatory surfaces.

    Science.gov (United States)

    2004-11-01

    The objective of this article to help dental professionals understand the changes in surface asepsis recommendations, to be able to classify environmental surfaces in the practice setting and successfully prevent or manage their contamination, as well as become familiar with the terminology used in discussing environmental surfaces and related infection control efforts. PMID:15651468

  4. Production of Chinese wolfberry draft beer%保健型枸杞纯生啤酒酿造方法的研究

    Institute of Scientific and Technical Information of China (English)

    赵春杰; 李正英

    2009-01-01

    将枸杞汁与麦芽汁以一定比例配料,经纯种发酵,以无菌过滤和无菌灌装技术生产出保健型枸杞纯生啤酒.通过实验确定了枸杞的最佳添加量和添加时间,优化了发酵工艺参数,制定出保健型枸杞纯生啤酒酿造工艺.%Using mixture of Chinese wolfberry and wort as raw materials, Chinese wolfberry draft beer was produced after pure fermentation, asepsis filtration and asepsis fill. The optimal content and time of adding Chinese wolfberry were obtained and the fermentation conditions optimized.

  5. Prevention of postoperative infections in ophthalmic surgery

    Directory of Open Access Journals (Sweden)

    Ram Jagat

    2001-01-01

    Full Text Available Postoperative endophthalmitis is a serious, vision-threatening complication of intraocular surgery. Better instrumentation, surgical techniques, prophylactic antibiotics and better understanding of asepsis have significantly reduced the incidence of this complication. Postoperative endophthalmitis may occur as an isolated event or as a cluster infection. Topical antibiotics, preoperative periocular preparation with povidone-iodine combined with a sterile operating room protocol significantly reduce the incidence of isolated postoperative endophthalmitis. The role of antibiotics in the irrigating fluid and subconjunctival antibiotics remains controversial. Cluster infections on the other hand are more likely to occur due to the use of contaminated fluids/viscoelastics or a breach in operating room asepsis. Prevention of postoperative endophthalmitis requires strict adherence to operating room norms, with all involved personnel discharging their assigned roles faithfully.

  6. Aortic valve replacement in a patient with systemic lupus erythematosus

    Directory of Open Access Journals (Sweden)

    Bhuvnesh Kansara

    2013-01-01

    Full Text Available Valvular heart disease in systemic lupus erythematosus (SLE is associated with substantial morbidity and mortality. Current therapy includes symptomatic measures and valve replacement. SLE can present major challenges because of accrued organ damage, coagulation defects and complex management regimes. The peri-operative goals are to maintain strict asepsis, avoid use of nephrotoxic drugs and thereby renal insult, and to promote early ambulation post-operatively.

  7. [Health problems of combatants during the First World War].

    Science.gov (United States)

    Lefort, Hugues; Ferrandis, Jean-Jacques; Tabbagh, Xavier; Domanski, Laurent; Tourtier, Jean-Pierre

    2014-06-01

    The First World War because of the use of new weapons, injured more than 3 500 000 people (500 000 in the face), more than diseases (tuberculosis, typhoid fever, etc.) or even weather circumstances. The healing of the war wounds through surgery undertook a significant evolution thanks to the use of asepsis and antiseptics. Mortality go down, opening the way to the physical and psychological rehabilitation of those injured by the war.

  8. The Life of John Wishart (1850–1926): Study of an Academic Surgical Career Prior to the Flexner Report

    OpenAIRE

    Claydon, Emily; McAlister, Vivian C.

    2012-01-01

    Background The 1910 Flexner Report on Medical Education in the United States and Canada is often taken as the point when medical schools in North America took on their modern form. However, many fundamental advances in surgery, such as anesthesia and asepsis, predated the report by decades. To understand the contribution of educators in this earlier period, we investigated the forgotten career of John Wishart, founding Professor of Surgery at Western University, London Ontario. Methods Archiv...

  9. ELIMINATION OF INTRACANAL INFECTION IN DOGS' TEETH WITH INDUCED PERIAPICAL LESIONS AFTER ROTARY INSTRUMENTATION: INFLUENCE OF DIFFERENT CALCIUM HYDROXIDE PASTES

    OpenAIRE

    Janir Alves Soares; Mário Roberto Leonardo; Léa Assed Bezerra da Silva; Mário Tanomaru Filho; Izabel Yoko Ito

    2006-01-01

    The aim of this study was to evaluate the antiseptic efficacy of rotary instrumentation associated with calcium hydroxide-based pastes prepared with different vehicles and antiseptics. Chronic periapical lesions were experimentally induced in 72 premolar root canals of four dogs. Under controlled asepsis, after initial microbiological sampling (A1), the root canals were instrumented using the ProFile system in conjunction with 5.25% sodium hypochlorite and the intracanal medication was placed...

  10. Search for microorganisms in antiseptic, disinfectant and detergent dispensers of a local health care unit from Northeast Portugal

    OpenAIRE

    Alves, Maria José; Pombo, Graça; Pereira, Ana; Conde, Andreia; Soares, Isabel; Rosmaninho, Marina; Ferreira, Isabel C. F. R.

    2015-01-01

    Introduction: Health care associated infections (HCAI) are among the main causes of mortality worldwide, and of the increase of internment time and related costs. Many HCAI acquired in hospitals result from improper disinfection practices of medical devices and surfaces of the hospital environment, as also insufficient asepsis of health professional’s skin [1,2]. Objectives: The main objectives of the present work were to verify the presence of microorganisms in antiseptic, ...

  11. The ratio in choosing access flap for surgical endodontics: a review

    OpenAIRE

    Grandi, C.; Pacifici, L.

    2009-01-01

    In Surgical Endodontics we face complex situations that require the knowlflap and application of both principles of endodontics (cleansing shaping and filling) in the root management, and the principles of surgery (asepsis, non harmfulness and hemostasis) in the phase of access to the root. Among the factors of surgical relevance that for which have been proposed with the largest number of alternatives is the design of access flap. That clearly indicates that not only one flap design can be i...

  12. Clinical relevance of surgical site infection as defined by the criteria of the Centers for Disease Control and Prevention.

    Science.gov (United States)

    Henriksen, N A; Meyhoff, C S; Wetterslev, J; Wille-Jørgensen, P; Rasmussen, L S; Jorgensen, L N

    2010-07-01

    Surgical site infection (SSI) is a common complication after abdominal surgery and the Centers for Disease Control and Prevention (CDC) criteria are commonly used for diagnosis and surveillance. The aim of this study was to evaluate whether SSI diagnosed according to CDC is clinically relevant (CRSSI) and whether there is agreement between evaluations according to the CDC criteria, the ASEPSIS score (Additional treatment, presence of Serous discharge, Erythema, Purulent exudate, Separation of the deep tissues, Isolation of bacteria and duration of Stay) and CRSSI. We included 54 patients diagnosed with SSI and a matched control group (N=46) without SSI according to the CDC criteria after laparotomy. Two blinded experienced surgeons evaluated the hospital records and determined whether patients had CRSSI, based on the following criteria: antibiotic treatment, surgical intervention, prolonged hospital stay or referral to an intensive care unit for SSI. The rate of CRSSI was 38 of 54 (70%) in patients with CDC-diagnosed SSI and none in patients without a CDC-diagnosed SSI. Sixty-one percent of the CDC-diagnosed SSIs were superficial, of which 48% were considered clinically relevant. There was substantial agreement between the CDC criteria and CRSSI [kappa=0.69; 95% confidence interval (CI): 0.55-0.83] and fair agreement between the ASEPSIS score and the CDC criteria (kappa=0.23; 95% CI: 0-0.49) and between the ASEPSIS score and CRSSI (kappa=0.39; 95% CI: 0.17-0.61). The CDC criteria represent a suitable standard definition for monitoring and identifying SSI, even if some cases of less clinically significant superficial SSI are included.

  13. BEST-PRACTICE GUIDELINES FOR FIELD-BASED SURGERY AND ANESTHESIA ON FREE-RANGING WILDLIFE. II. SURGERY.

    Science.gov (United States)

    Fiorello, Christine V; Harms, Craig A; Chinnadurai, Sathya K; Strahl-Heldreth, Danielle

    2016-04-01

    The principles of surgical asepsis apply to field surgeries with few exceptions. The minimum level for performance of surgeries in the field on free-ranging animals should be the same as for domestic animals undergoing surgery in animal hospitals. Surgeries in the field are typically done as part of research and management projects and usually involve a combination of biologists and veterinarians with the possibility of conflicts in scientific cultures. This article outlines a minimum standard of care for field surgeries and will serve as a resource for Institutional Animal Care and Use Committees and biologists and veterinarians planning projects that involve surgeries on free-ranging wildlife in field conditions.

  14. Acute pelvic inflammatory disease in a sub-Saharan country: a cross sectional descriptive study

    Directory of Open Access Journals (Sweden)

    Elie Nkwabong

    2015-06-01

    Conclusions: Acute PID is common among young, single women with multiple sexual partners, who should be regularly screened for the various sexually transmissible infections. The micro-organisms frequently responsible for acute PID were genital tract mycoplasmas, whose identification should be included among the routine tests done to women with acute PID. Cases of acute PID due to intra-uterine procedures reminds us that adequate asepsis should be observed during these procedures. [Int J Reprod Contracept Obstet Gynecol 2015; 4(3.000: 809-813

  15. [Johannes Anton von Mikulicz-Radecki (1850-1905). His contributions to the development of urology].

    Science.gov (United States)

    Zajaczkowski, T; Zamann, A M

    2010-02-01

    On June 14, 1905, Johann Anton von Mikulicz-Radecki, one of the most famous surgeons of the time, died. He was a disciple of famous Theodor Billroth of Vienna and the teacher of Ferdinand Sauerbruch in Breslau. Mikulicz-Radecki's merits in the field of the history of medicine are based on his achievements in developing the system of antisepsis and asepsis. He also devoted himself to urological surgery, and his name is linked with diseases, operations, and construction of new medical instruments. Mikulicz-Radecki created two surgical schools, one in Cracow and a second one in Breslau, where he worked for over 15 years until his death.

  16. Surgery, sterilization and sterility.

    Science.gov (United States)

    Green, V W

    1993-03-01

    The history of sterilization was not linked from the first with surgery. Surgery came first, fully 600 years before the principles of asepsis and anesthesia were even introduced in the middle of the 1800s. Also in the 1800s, the beginnings of thermal sterilization were being developed in the food industry. The basic principles of antisepsis and prevention of wound suppuration, including the destruction of germs on instruments, dressings, the hands of the surgeon and his assistants, and everything else in contact with the wound were clearly elucidated by Lister in the 1870s and remain the inviolate principles of surgical asepsis today. In general, the marriage between the surgeons and the sterilizers was a successful one; the major handicap to eternal bliss and harmony, however, was an incompatibility between the partners. As in many marriages, the partners made unwarranted demands upon each other, and became frustrated when these demands were unfulfilled. The field of surgical sterilization and surgical safety is less confused by technical inconsistencies than it is by semantic nightmares, such that we will never reach a universal definition of sterility. However, we do not really need a universal definition of sterility. Rather we should learn how to translate sterility tests in terms of the real world infections hazards.

  17. Outbreak of Achromobacter xylosoxidans and Ochrobactrum anthropi Infections after Prostate Biopsies, France, 2014

    Science.gov (United States)

    Cassier, Pierre; Dananché, Cédric; Hulin, Monique; Dauwalder, Olivier; Rouvière, Olivier; Bertrand, Xavier; Perraud, Michel; Bénet, Thomas; Vanhems, Philippe

    2016-01-01

    We report an outbreak of healthcare-associated prostatitis involving rare environmental pathogens in immunocompetent patients undergoing transrectal prostate biopsies at Hôpital Édouard Herriot (Lyon, France) during August 13–October 10, 2014. Despite a fluoroquinolone-based prophylaxis, 5 patients were infected with Achromobacter xylosoxidans and 3 with Ochrobactrum anthropi, which has not been reported as pathogenic in nonimmunocompromised persons. All patients recovered fully. Analysis of the outbreak included case investigation, case–control study, biopsy procedure review, microbiologic testing of environmental and clinical samples, and retrospective review of hospital records for 4 years before the outbreak. The cases resulted from asepsis errors during preparation of materials for the biopsies. A low-level outbreak involving environmental bacteria was likely present for years, masked by antimicrobial drug prophylaxis and a low number of cases. Healthcare personnel should promptly report unusual pathogens in immunocompetent patients to infection control units, and guidelines should explicitly mention asepsis during materials preparation. PMID:27434277

  18. Work conditions and occupational health of dentists in Brazilian Public Health System

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    Suzely Adas Saliba Moimaz

    2014-03-01

    Full Text Available Introduction: work healthy conditions are essential for a great professional performance. Objective: To verify the perception of dentists regarding structural and healthy conditions of dental offices in the Brazilian Public Health System (SUS and their satisfaction with work and public job. Material and Method: In this cross-sectional study, type inquiry, 24 Brazilian dentists were interviewed. The follow variables were asked: cleaning and asepsis; maintenance and time of use of dental equipment; satisfaction with work and public job. Results: The professionals were "satisfied" or "very satisfied" with cleaning and asepsis (66.67%; Dental equipment (54.17%, reflectors (54.17% and the dental chairs (54.17% had more than 24 years of use; 20% of all professionals said that the equipment had maintenance but only to fix them. Of total, 58.33% had already given no attendance for patients and broken equipment was the most frequent cause (92.86%. It was observed satisfaction with work (79.1% and public job (95.83%. It was concluded that dentists who worked in SUS were satisfied with public job, although they had said the need for improvement on structural and healthy work conditions.

  19. Establishment of an operating room committee and a training program to improve aseptic techniques for rodent and large animal surgery.

    Science.gov (United States)

    Héon, Hélène; Rousseau, Nathalie; Montgomery, Jane; Beauregard, Gilles; Choiniére, Manon

    2006-11-01

    Investigators of our research facility generally accept the concept of asepsis as an important component of adequate surgical care for animals. However, they experience difficulties putting it into practice, especially in the case of rodents. The reasons for this are inconvenience, cost, and lack of training. To better assist investigators in the implementation of aseptic surgical techniques in their laboratories, we have created an Operating Room (OR) Committee modeled after OR committees found in human hospitals. A reconstructive surgeon, a veterinarian, a research scientist, a nurse involved in the training of OR personnel, interns, graduate students, and an animal health technician were chosen as committee members in light of their OR and animal care expertise. The first task of the OR Committee was to establish institutional guidelines for aseptic surgery, taking into account the costs imposed on research budgets by these procedures. The OR Committee also supports a complete training program in aseptic surgery techniques, which consists of lectures, a training manual, videos, and a practical course. Furthermore, when experimental procedures require specialized equipment, the OR Committee collaborates with researchers to develop strategies to achieve asepsis. This OR Committee and the training program proved to be important tools to promote and improve the quality of animal care during surgery.

  20. Pathogenicity of aseptic Bursaphelenchus xylophilus.

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    Li-hua Zhu

    Full Text Available Pine wilt is a disease of pine (Pinus spp. caused by the pine wood nematode (PWN, Bursaphelenchus xylophilus. However, the pathogenic mechanism of pine wilt disease (PWD remains unclear. Although the PWN was thought to be the only pathogenic agent associated with this disease, a potential role for bacterial symbionts in the disease process was recently proposed. Studies have indicated that aseptic PWNs do not cause PWD in aseptic pine trees, while PWNs associated with bacteria cause wilting symptoms. To investigate the pathogenicity of the PWN and its associated bacteria, 3-month-old microcuttings derived from certain clones of Pinus densiflora Siebold & Zucc. produced in vitro were inoculated under aseptic conditions with aseptic PWNs, non-aseptic PWNs and bacteria isolated from the nematodes. Six-month-old aseptic P. densiflora microcuttings and 7-month-old P. massoniana seedlings were also inoculated under aseptic conditions with aseptic PWNs and non-aseptic PWNs. The results showed that the aseptic microcuttings and seedlings inoculated with aseptic PWNs or non-aseptic PWNs wilted, while those inoculated with bacterial isolates did not wilt. Nematodes were recovered from wilted microcuttings and seedlings inoculated with aseptic PWNs and non-aseptic PWNs, and the asepsis of nematodes recovered from aseptic PWN-inoculated microcuttings and seedlings was reconfirmed by culturing them in NB liquid medium at 30°C for more than 7 days. Taken together, the results indicate that the asepsis of PWN did not cause the loss of pathogenicity.

  1. 继发性腰椎间隙感染合并肺脓肿的救治%The Cure of Secondary Cases Interspace of Lumbar Vertebrae Infection Complicated Pulmonary Abscess

    Institute of Scientific and Technical Information of China (English)

    张淼; 顾苏俊

    2009-01-01

    OBJECTIVE Research the therapeutic effectiveness of combination Imipenem and teicoplanin to treat septicemia due to interspace of lumbar vertebrae Infection complicated pulmonary abscess and the method of diagnosis, prevention and cure of Secondary interspace of lumbar vertebrae Injection. METHODS The successful cure case-report that severe postoperative interspace of lumbar vertebrae infection complicated blood-borne pulmonary abscess used small needle knife to treat Lumbar intervertebral disc protrusion (LIDP) were reviewed.RESULTS Unstandard asepsis technique and invasive operation long time operation, too mang damage of tissue in operation, patient weak, diabetes, glucocorticoid, lacking postoperative anti infection therapy etc. Lumbocrural pain were found aggravated, high fever, blood sedimentation increased, CRP heightened. CONCLUSIONS Strict asepsis,sufficient preoperotive preparation, Standard operation, less damage, can reduce interspace of lumbar vertebrae infection rate. Strict asepsis and less damage in the operation can reduce the incidence of intervertebral disc space infection.%目的 探讨亚胺培南/西司他丁与替考拉宁联合应用治疗腰椎间隙感染后败血症合并肺脓肿的疗效以及继发件腰椎间隙感染的发病特点、诊断及防治方法.方法 通过对1例小针刀治疗腰椎间盘突出后导致重度腰椎间隙感染合并血源性肺脓肿患者的成功救治进行同顾性总结分析.结果 无菌观念不严格、介入性操作不规范、手术时间长、术中组织损伤多、患者基础条件差、糖尿病、应用糖皮质激素、术后抗炎不足等因素有关;患者术后3 d腰腿痛加剧.高热,血沉增快,C-反应蛋白增高.结论 严格无菌观念,充分术前准备,术中操作规范减少损伤可减少腰椎间隙感染的发生.

  2. [Nosocomial urinary infections].

    Science.gov (United States)

    Butreau-Lemaire, M; Botto, H

    1997-09-01

    The concept of nosocomial urinary tract infection now corresponds to a precise definition. It is generally related to bladder catheterization, constitutes the most frequent form of nosocomial infection (30 to 50% of infections), and represents the third most frequent portal of entry of bacteraemia. The organism most frequently isolated is Escherichia coli; but the flora is changing and the ecological distribution is continually modified. Despite their usually benign nature, these nosocomial infections can nevertheless influence hospital mortality; they increase the hospital stay by an average of 2.5 days and their treatment represents a large share of the antibiotic budget. Prevention of these infections is therefore essential, with particular emphasis on simple and universally accessible measures: very precise indications for vesical catheterization, use of closed circuit drainage, maximal asepsis when handling catheters, after washing the hands.

  3. Standard guidelines for setting up a dermatosurgery theatre

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    Rajendran S

    2009-08-01

    Full Text Available Introduction, definition, rationale and scope: Dermatologists in India are now increasingly performing surgical and cosmetic procedures in their practice. This calls for minimum standards at the national level with the main focus of patient safety and hence the guidelines for setting up a dermatosurgical theatre. Facility: The dermatosurgery theatre can be created in either physician′s clinic, or a hospital depending on the procedure to be performed. The dermatosurgery theatre requires careful planning with regards to - location, dimension, shell design, lighting, electrical requirements, operation table, chair, trolley, surgical instruments, sterilization of devices, asepsis and advanced life support. Apart from physical considerations, other considerations including theatre etiquettes, consent for surgery, safety of dermatosurgeon, theatre staff and lastly biomedical waste management should be looked into. These issues are discussed in detail in the recommendations.

  4. Méningite après rachianesthésie

    Science.gov (United States)

    Mouchrif, Issam; Berdaii, Adnane; Labib, Ismail; Harrandou, Moustapha

    2016-01-01

    Les méningites sont des complications rares mais graves des anesthésies péridurales et des rachianesthésies. Les méningites bactériennes sont essentiellement dues à des cocci à Gram positif, ce qui sous-entend une contamination exogène pouvant être le témoin d'une faute d'asepsie. L’évolution est le plus souvent favorable sous traitement, mais au prix d'un accroissement des dépenses de santé et parfois d'importantes séquelles neurologiques. Nous présentons un cas de méningite bactérienne au décours d'une rachianesthésie effectué pour césarienne. PMID:27642477

  5. Appearance of urology at the beginining of xxth century -from the general surgeon up to the highly trained specialist

    Directory of Open Access Journals (Sweden)

    Ignjatović Ivan M.

    2003-01-01

    Full Text Available Evolvement of urology as a separate scientific discipline depends on several factors. Endoscopic diagnostics, radiological diagnostics, and operative techniques in general surgery, had provided an ability to perform more complex and longer operations. Urology had evolved from the great schools of surgery in Germany and France, and their the most important surgeons, who were intersted in urological surgery. The first endoscope was introduced in 1806, and received today’s form in 1879. Application of "X" rays in medicine started in 1895, and it was later applied as a cystography, retrograde pyelography as well as intravenous urography. The most important thing for the operative technique evolution were application of anhestesia, asepsis and new hemostatic devices. During the one century long development, urology had passed the way from completely unknown field, up to the discipline with the best diagnostic preciseness.

  6. District nurses' and aseptic technique: where did it all go wrong?

    Science.gov (United States)

    Unsworth, John

    2011-01-01

    Concerns have been raised about whether it is possible to perform aseptic procedures within a community setting. Hallett (2000) has described how community nurses often have a fatalistic view about whether such procedures can really be performed at home. At the same time there has been an increase in the number of patients being cared for at home who need interventions which must adhere to the principles of asepsis. While it has been acknowledged for some time that community nurses must be resourceful and adapt the procedure, the view that community nurses cannot really perform aseptic procedures is a fairly new phenomenon. This article explores the reasons why concerns about the performance of aseptic procedures in the community may have arisen and what steps can be taken to address these concerns to ensure that care at home is both safe and effective.

  7. Use of subepithelial conjunctive tissue graft in root covering

    Directory of Open Access Journals (Sweden)

    Denis Clemente Rodrigues

    2010-04-01

    Full Text Available Gingival recession may cause cosmetic changes and root hypersensitivity, which can alter mastication and tooth brushing, and thus contribute to the progression of periodontal disease. Treatment of gingival recessions with subepithelial conjunctive tissue grafting is very predictable due to its bilaminar nature. However, some factors may influence the success of the procedure, among them: selection of the lesion; characteristics of the defect with regard to the depth and width of the recession, the deeper and wider the recession, the worse the prognosis, and also the presence of restorations. Furthermore, it is necessary to control the etiologic factors of recessions, such as inflammation and traumatic brushing, control of systemic factors, occlusal trauma, smoking and harmful habits, technical considerations and asepsis. The success of the procedure must be based on observing the possible factors that might influence their predictability, as well as respecting the basic principles of mucogingival surgery.

  8. Division of twelve packages in nuclear medicine: feasibility under different conditions of temperature; Fractionnement de douze trousses en medecine nucleaire: faisabilite sous differentes conditions de temperature

    Energy Technology Data Exchange (ETDEWEB)

    Joyes, P

    1998-10-12

    The aim of this study is to experiment with the feasibility of the fractionation of radiopharmaceuticals cases on a majority of cases for labelling to technetium 99m at a temperature of -16 celsius degrees and -80 celsius degrees. The cases are pharmaceuticals to inject. They must have an obligatory asepsis. At every labelling, a monitoring of the radiochemical purity is completed by thin layer chromatography, by chromatography on paper, by high performance liquid chromatography. By the technique of fractionation studied here, a conservation at -16 degrees Celsius is enough for the diphosphonate, the albumin aggregates, the tetrofosmin and the phytate. for the H.M.P.A.O., the M.A.G.3, the D.M.S.A. and the biliary tracers it is necessary to have a conservation mean at very low temperature. The fractionation is a technique in nuclear medicine that dose not give any difficulties and can become a usual practice in nuclear medicine. (N.C.)

  9. PROGNOSTIC FACTORS DETERMINING MORTALITY IN SURGICAL NEONATES

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    Vivek Manchanda

    2012-01-01

    Full Text Available Background: To assess the prognosis of surgical neonates at admission and the factors responsible for mortality in neonates.Material and Methods: A prospective study was conducted in a tertiary level hospital over 15 months and various clinical and biochemical parameters were collected and analyzed using STATA® and SPSS®.Results: On multivariate analysis of 165 neonates, early gestational age, respiratory distress and shock at presentation were the factors of poor prognosis in neonates. The factors could be related to poor antenatal care and sepsis acquired before transfer of the baby to the nursery. Conclusion: The improvement in antenatal care and asepsis during transfer and handling the babies is of utmost importance to improve the prognosis of surgical neonates.

  10. Plant development in space: Observations on root formation and growth

    Science.gov (United States)

    Levine, H. G.; Kann, R. P.; Krikorian, Abraham D.

    1990-01-01

    Root growth in space is discussed and observations on root production from plants flown as part of the Chromex project that were defined as to their origin, stage of development and physiological status, are presented. Roots were generated from fully differentiated, aseptically maintained individuals of Haplopappus gracilis (Compositae) under spaceflight conditions. Results are compared for tissue culture generated plantlets and comparably sized seedling clone individuals, both of which had their roots trimmed on Earth before they were loaded into NASA's plant growth unit and subjected to a 5 day shuttle flight (STS-29). Asepsis was maintained throughout the experiment. Overall root production was 40 to 50 percent greater under spaceflight conditions than during ground control tests. However, root formation slowed down towards the end of the flight. This decrease in new roots did not occur in the ground controls that sought to simulate flight except for microgravity.

  11. A novel approach to the statistical evaluation of media fill tests by the difference from no contamination data.

    Science.gov (United States)

    Kawamura, Kunio; Abe, Hiroshi

    2004-01-01

    It is obvious that, in the media fill test and process simulation test, positive numbers in total fills should not have any significant difference from zero or asepsis. There are many reports concerning the definition of "sterility" or "asepsis." However, any scientific and practical methods to demonstrate "no significant difference from zero" have not been reported up to now. The existing criteria, such as "less than 0.1%," "less than 0.05%," and "less than two positives" are not appropriate to assure the integrity of processes, and sometimes lead to erroneous results. The purpose of this report is to demonstrate novel, reasonable and practical methods and criteria based on scientific and statistical consideration. According to the ISO 13408-1 Aseptic Processing of Health Care Products, Part 1 (1998), General Requirement for Aseptic Processing, the action level for the number of positive units in media fill tests is specified as 0.1%, and the alert level is 0.05%. In this paper it is shown that the existing ISO standard and other official methods are inappropriate in that zero contaminated units (sterile product) is outside the confidence range of probable distribution of contaminated units, even though the contaminated units are less than 0.1% in larger numbers of fills, and even less than 0.05%. This indicates that the limit of 0.1% or 0.05% is inappropriate in cases of larger numbers of fills. For sterile products, the number of contaminated units other than "zero" at the statistical confidence range must be judged to be contaminated units in process and as non-sterile. In order to harmonize this criteria-"no significant difference from zero"-with the existing criteria, the new criteria may be combined with only the existing criteria of 0.05% in smaller number of fills.

  12. Manufacture use and invention of protected transport carts for pollutants in operating rooms%手术室污染物品密闭转运车的使用与管理

    Institute of Scientific and Technical Information of China (English)

    田继红

    2012-01-01

    OBJECTIVE To solve the problems of the collection and protected transport of the postoperative pollutants in asepsis area of the operating rooms with single aisle. METHODS A cart was designed for protecting transport of the pollutants in the operating room. RESULTS The effectiveness of the cart has been well proved by applying it in asepsis area in the operating room. It could reduce the risks of infection environment in operating area,ameliorate the OR management and save the operation cost and improve the nursing quality and efficiency. CONCLUSION We conclude that because of its advantages of safety, simple operation and less cost, the protected transport cart is a necessary medical facility in the operating room with single aisle.%目的 解决单走廊洁净手术部术后污染物品的收集与密闭转运的问题.方法 自行设计手术室污染物品与废弃物品密闭转运车.结果 自2006年10月-2011年5月临床使用效果证明其实现了单走廊洁净手术部术后污染物品的快速收集与密闭转运;规避了单走廊洁净手术部环境污染的风险;促进了护理管理的持续改进;节省了手术运营成本,提高了护理工作的质量和效率.结论 因其安全可靠、操作简单、方便快捷、经济实用,而成为单走廊洁净手术部感染控制之必备的医疗用品.

  13. Sterilization Conditions for Tissue Culture of Stem in Rubia cordifolia L.%茜草茎段组织培养灭菌方法研究

    Institute of Scientific and Technical Information of China (English)

    于相丽; 李勇慧; 王静

    2011-01-01

    研究了不同灭菌剂(0.1%HgCl2、10%NaClO、10%H2O2)、不同灭菌时间、二步灭菌法对茜草不同年龄的茎段污染率和愈伤组织诱导率的影响.结果表明:75%酒精处理30 s+10%NaClO灭菌20 rain效果最好,污染率20%,愈伤组织诱导率为35%;二步灭菌法不适合于茜草茎段的灭菌;幼嫩茜草茎段愈伤组织诱导率最高,达55%.%The different age stems from Rubia cordifolia L.were used as explants to study the effects of different bactericides (0.1% HgCl2, 10% NaClO, 10% H2O2), different sterilization times, and two-step asepsis on pollution of explants and induction of callus.The results showed that the method of 75 % alcohol 30 s+10% NaClO 20 min was the best and the pollution rat was 20%,induction rate was 35%; the method of two-step asepsis was suitable for stems of Rubia cordifolia L.; the young stems induction rate of callus was the highest up to 55%.

  14. Quality control of the kit and injection of 99 Tcm-TRODAT-1 as a dopamine transporter imaging agent%多巴胺转运蛋白显像剂99Tcm -TRODAT-1药盒及注射液的质量控制

    Institute of Scientific and Technical Information of China (English)

    唐婕; 陈正平; 李晓敏; 刘春仪; 黄旭荃; 李卫一; 邹美芬; 徐希杰

    2011-01-01

    Objective To study the quality control methods for 99Tcm-TRODAT-1 kit and injection.Methods The appearance,pH,contents of the bases,the labeling yield,asepsis and bacterial endotoxins of 99Tcm-TRODAT-1 kits from three different batches were examined.The kit stability was estimated under different conditions.The transparence,pH,radiochemical purity,half-life,asepsis and bacterial endotoxins of 99Tcm-TRODAT-1 injection were tested.Results The 99Tcm-TRODAT-1 kit and injection were both achromous and transparent,with pH values being 5.9 ± 0.1 and 5.5 - 7.0 respectively.The contents of stannous chloride and TRODAT-1 were stable.The labeling yield of the kit and the radiochemical purity of the injection were both ≥95%.The asepsis test demonstrated that the characters of 99Tcm-TRODAT-1 kit and injection were qualified.TRODAT-1 kit was stable at 0 -4 ℃ for 6 months or at room temperature (20 -25 ℃ )for 10 days,and the radiochemical purity of the injection was still > 90% at room temperature for 8 hours.Conclusions The quality control methods for 99Tcm-TRODAT-1 kit are simple and practical.The kit and injection are qualified and can be used for clinical application.%目的 对自制多巴胺转运蛋白显像剂99Tcm-TRODAT-1药盒及注射液进行质量控制研究.方法 对3批99 Tcm-TRODAT-1药盒及注射液进行澄清度、pH值、氯化亚锡与TRODAT-1含量、标记率、放化纯、半衰期测定,无菌以及细菌内毒素检查,可见异物、不溶性微粒检查,并分析药盒在不同存放环境下的稳定性.结果 99Tcm-TRODAT-1药盒澄清度好,pH值为5.9±0.1,药盒中氯化亚锡与TRODAT-1含量稳定,标记率大于95%.药盒在0~4℃下6个月、室温(20~25℃)下10 d内稳定性好.注射液澄清透明、pH值在5.5~7.0之间,放化纯≥95%,室温(20 ~25℃)下8h内放化纯>90%.药盒及注射液无菌、细菌内毒素检查合格.结论 自制的99Tcm-TRODAT-1药盒及注射液的质量控制方法简便易

  15. Aseptic technique in microgravity.

    Science.gov (United States)

    McCuaig, K

    1992-11-01

    Within the next decade, the United States will launch a space station into low Earth orbit as a preliminary step toward a manned mission to Mars. Provision of asepsis in the unique microgravity environment, essential in operative and invasive procedures, is addressed. An assessment of conventional terrestrial aseptic methods and possible modifications for a microgravity environment was done during the microgravity portion of parabolic flight on NASA KC-135 aircraft. During 110 parabolas on three flight days, a "surgical team" (surgeon, scrub nurse and circulating nurse) using a life size mannequin fastened to a prototype surgical "work station" (operating table), evaluated open and closed gloving (ten parabolas), skin preparation (six parabolas), surgical scrub methods (24 parabolas), gowning (22 parabolas) and draping (48 parabolas). Evaluated were povidone iodine solution, 1 percent povidone iodine detergent, Chloroxylenol with detergent, wet prep soap sponge, a water insoluble iodophor polymer (DuraPrep, 3M), disposable towels, disposable and reusable gowns, large and small disposable drapes with and without adhesive edges, disposable latex surgeon's gloves with and without packaging modifications and restraint mechanisms (tether, swiss seat, waist and foot restraint devices, fairfield and wire clamps and clips). Ease of use, provision of restraint for supplies and personnel and waste disposal were assessed. The literature was reviewed and its relevance to the space environment discussed, including risk factors, environmental contamination, immune status and microbiology. The microgravity environment, limited water supply and restricted operating area mandated that modifications of fabrication and packaging of supplies and technique be made to create and preserve asepsis. Material must meet stringent flammability and off-gassing standards. Either a chlorhexidine or povidone iodine detergent prepackaged brush and sponge would provide an adequate scrub plus

  16. A Study to Assess the Association of Aseptic Practices Being Followed and its Effect on the Overall Outcome of Selected Special Care Newborn Units (SCNUs of Madhya Pradesh at Different Levels.

    Directory of Open Access Journals (Sweden)

    Mahore RK

    2015-04-01

    Full Text Available The neonatal mortality rate in India is high and stagnant. Special Care Newborn Units (SCNUs have been set up at different levels of Health Care Delivery System to provide quality newborn-care services in several hospitals to meet this challenge. Many units are located in the districts where the burden of neonatal deaths is high, and access to special newborn care is poor. The study was conducted to assess the functioning of SCNUs in six centers of India. The evaluation was based on an analysis of secondary data from the six units that had been functioning for at least three year. A cross-sectional survey was also conducted to assess the availability of Quality care and Aseptic practices implemented at different levels of SNCUs. Descriptive statistics were used for analyzing the inputs (Quality care and aseptic practices and outcomes (morbidity and mortality. The rate of mortality among admitted neonates was taken as the key outcome variable to assess the performance of the units. Chi-square test was used for analyzing the trend of case-fatality rate over a period of 3 years considering the first year of operationalization as the base. Correlation coefficients were estimated to understand the possible association of case-fatality rate with factors, such as bed:doctor ratio, bed:nurse ratio, average duration of stay, and bed occupancy rate, and the asepsis score was determined. The rates of admission increased from a median of 16.7 per 100 deliveries in 2012 to 19.5 per 100 deliveries in 2014. The case-fatality rate shows progressive decline in all the units in Last 3 years. Proportional mortality due to sepsis and low birthweight (LBW declined significantly over two years (LBW <2.5 kg. The major reasons for admission and the major causes of deaths were birth asphyxia, sepsis, and LBW/prematurity. The units had a varying nurse:bed ratio (1:1-1:2.14. The bed occupancy rate ranged from 83% to 121% (median 115%, and the average duration of stay

  17. Effects of“0.618 gold cup”and different substrates on rooting of tissue culture seedling in Camellia oleifera%“0.618黄金杯”和不同基质对油茶组培苗生根的影响

    Institute of Scientific and Technical Information of China (English)

    蔡冬元

    2015-01-01

    To solve the different problem of root system development of tissue culture seedlings in Camellia oleifera, tissue culture seedling from C. oleifera asepsis short-branches were planted in“gold cups”made with the“0.618 gold level”principle and traditional nutrition cottage cups, iflled with ifne sand, red soil and mixed substrate of pearl stone and plant ash (1︰1), and the effects of different nutrition cups and substrates on rooting of tissue culture seedlings in C. oleifera were compared. The results showed that taking the mixture of pearl stone and plant ash (1︰1) as substrate, tissue culture seedling from C. oleifera asepsis short-branches in“gold cups”made with the“0.618 gold level”principle, had 10.68 roots per plant within 30 days, average root length was 7.52 cm, and the rooting rate reached 100%.%为了解决油茶组培苗根系发生困难的问题,将油茶无菌短枝组培苗分别扦插在运用“0.618黄金水位”原理制作的“黄金杯”和扦插常用的传统营养杯中,两种营养杯中分别填入以1︰1的珍珠岩与草炭的混配基质、细河沙及红心土,就不同营养杯和不同基质处理对油茶组培苗生根的影响情况进行了对比试验。结果表明:采用1︰1的珍珠岩与草炭的混配基质,将油茶无菌短枝组培苗扦插在运用“0.618黄金水位”原理制作的“黄金杯”中,可使油茶组培苗在30天内发生出10.68条/株、平均根长为7.52㎝的根系,其发根率为100%。

  18. [Prophylactic antibiotics in neurosurgery].

    Science.gov (United States)

    Iacob, G; Iacob, Simona; Cojocaru, Inimioara

    2007-01-01

    Because of a low risk of infection (around 2-3%), prophylactic use of antibiotics in neurosurgery is a controversial issue. Some neurosurgeons consider that there are strong arguments against the use of antimicrobials (promotion of antibiotic-resistant strains of bacteria, superinfection and adverse drug reactions) and meticulous aseptic techniques could be more usefully than prophylactic antibiotics. On the other hand, despite of being rare, the consequences of a neurosurgical infection can be dramatic and may result in a rapid death, caused by meningitis, cerebritis, abscess formation or sepsis. Clinical studies emphasized that the most important factors influencing the choice of antibiotic prophylaxis in neurosurgery is the patient's immune status, virulence of the pathogens and the type of surgery ("clean contaminated"--procedure that crosses the cranial sinuses, "clean non-implant"--procedure that does not cross the cranial sinuses, CSF shunt surgery, skull fracture). Prophylaxis has become the standard of care for contaminated and clean-contaminated surgery, also for surgery involving insertion of artificial devices. The antibiotic (first/second generation of cephalosporins or vancomycin in allergic patients) should recover only the cutaneous possibly contaminating flora (S. aureus, S. epidermidis) and should be administrated 30' before the surgical incision, intravenously in a single dose. Most studies pointed that identification of the risk factors for infections, correct asepsis and minimal prophylactic antibiotic regimen, help neurosurgeons to improve patient care and to decrease mortality without selecting resistant bacteria. PMID:18293694

  19. Five millennia of wound care products--what is new? A literature review.

    Science.gov (United States)

    Mouës, Chantal M; Heule, Freerk; Legerstee, Ron; Hovius, Steven E R

    2009-03-01

    The first wound an wound treatments were described five millennia ago. Since then, various principles of wound care have been passed on from generation to generation. In contrast to large numbers of general technological inventions over the last 100 years, progress beyond ancient wound care practices is a recent phenomenon. It is essential to know the historical aspects of wound treatment (both successes and failures) in order to continue this progress and provide future direction. A survey of the literature shows that concepts such as "laudable pus" persisted for hundreds of years and that lasting discoveries and meaningful progress did not occur until grand-scale manufacturing and marketing started. Landmarks such as understanding the principles of asepsis/antisepsis, fundamental cellular research findings, knowledge about antibiotics/antimicrobials, moist wound healing, and the chemical and physical processes of wound healing have provided the foundation to guide major developments in wound management, including available evidence-based guidelines. Although research regarding interaction of basic wound management principles remains limited, the combined efforts of global research and clinical groups predict a bright future for improved wound management. PMID:19359707

  20. Musculoskeletal interventional radiology: ultrasound and CT.

    Science.gov (United States)

    Martel Villagrán, J; Bueno Horcajadas, Á; Agrela Rojas, E

    2016-05-01

    We aim to describe imaging-guided (ultrasound and CT) interventional techniques in the musculoskeletal system that can be performed by general radiologists, whether in hospitals, primary care clinics, private offices, or other settings. The first requirement for doing these procedures is adequate knowledge of the anatomy of the musculoskeletal system. The second requirement is to inform the patient thoroughly about the technique, the risks involved, and the alternatives available in order to obtain written informed consent. The third requirement is to ensure that the procedure is performed in accordance with the principles of asepsis in relation to the puncture zone and to all the material employed throughout the procedure. The main procedures that can be done under ultrasound guidance are the following: fine needle aspiration cytology (FNAC), core needle biopsy (CNB), diagnostic and/or therapeutic arthrocentesis, drainage of juxta-articular fluid collections, drainage of abscesses, drainage of hematomas, treatment of Baker's cyst, treatment of ganglia, treatment of bursitis, infiltrations and treatment of plantar fasciitis, plantar fibrosis, epicondylitis, Achilles tendinopathy, and Morton's neuroma, puncture and lavage of calcifications in calcifying tendinopathy. We also review the following CT-guided procedures: diagnosis of spondylodiscitis, FNAC of metastases, arthrography, drainages. Finally, we also mention more complex procedures that can only be done in appropriate settings: bone biopsies, treatment of facet joint pain, radiofrequency treatment. PMID:27134018

  1. [Not Available].

    Science.gov (United States)

    Siah, S; Baite, A; Bakkali, H; Atmani, M; Ababou, K; Ihrai, H

    2009-09-30

    Le syndrome de Lyell ou nécrolyse épidermique toxique (NET) est une pathologie très grave des dermatoses bulleuses d'étiologie médicamenteuse. Il se caractérise par une nécrose aiguë de l'épiderme sur toute la hauteur du corps muqueux. L'aspect clinique de la NET est celui d'une brûlure étendue du deuxième degré profond. A ce tableau s'associent constamment des lésions muqueuses et une atteinte multiviscérale qui aggrave le pronostic. Nous rapportons deux cas de NET qui illustrent l'importance d'une prise en charge précoce et multidisciplinaire de ces patients atteints au sein d'un service de réanimation des brûlés, dont les fondements reposent sur l'asepsie rigoureuse, l'apport hydroélectrolytique et nutritionnel, la prévention de l'infection et son traitement par une antibiothérapie adaptée, et un nursing et des soins locaux. L'efficacité supposée des immunoglobulines intraveineuses ne repose que sur des cas isolés et il n'y a pas encore d'études randomisées. PMID:21991170

  2. Factors predisposing to wound infection in cardiac surgery. A prospective study of 517 patients.

    Science.gov (United States)

    Wilson, A P; Livesey, S A; Treasure, T; Grüneberg, R N; Sturridge, M F

    1987-01-01

    Postoperative wound infection can greatly prolong hospital stay after cardiac surgery, so the identification of predisposing factors may help in prevention or early institution of treatment. Transfer of organisms from the leg to the sternum during coronary artery surgery has been proposed as a major additional cause of sepsis. The definition of wound infection is not standardised and therefore makes comparison between centres difficult. In a prospective study of 517 patients, a wound scoring method (ASEPSIS) has been used to register all abnormal wounds to maximise the chances of identifying factors predisposing to infection. Abnormal healing was noted in 99 (19%) sternal wounds and 29 (8%) leg wounds. Obesity was the principal risk factor (P less than 0.005). Diabetes, reoperation, length of preoperative hospital stay, age, sex, or previous cardiac surgery had little effect on wound healing. The range of bacteria isolated from chest wounds after coronary artery surgery was similar to that after valvular surgery, but the rate of isolation was significantly greater. With careful attention to technique, leg wound infection rarely presented a clinical problem and did not appear to be a source of bacteria infecting the chest wound.

  3. The quality of measurement of surgical wound infection as the basis for monitoring: a systematic review.

    Science.gov (United States)

    Bruce, J; Russell, E M; Mollison, J; Krukowski, Z H

    2001-10-01

    Comparison of postoperative surgical wound infection rates between institutions and over time is only valid if standard, valid and reliable definitions are used. The aim of this review was to assess evidence of validity and reliability of the definition and measurement of surgical wound infection. A systematic review was undertaken of prospective studies of surgical wound infection published over a seven-year period; 1993-1999. The information extracted from individual studies included: definition of surgical wound infection; details of wound assessment scale, scoring or grading scale systems; and evidence of assessment of validity, reliability and feasibility of identified definitions and grading systems. Two independent reviewers appraised 112 prospective studies, 90 of which were eligible for inclusion; eight studies assessed validity and/or reliability. Forty-one different definitions of surgical wound infection were identified, five of which were 'standard' definitions proposed by multi-disciplinary groups. Presence of pus was the most frequently used single component of any definition; the CDC definitions of 1988 and 1992 were the most widely implemented standard definitions; and the ASEPSIS wound assessment scale was the most frequently used quantitative grading tool. Only two formal validations of a definition were found, and six studies of reliability. This review highlights the extent of variation in definition of surgical wound infection used in clinical practice, and the need for validation of both content and organization of a surveillance system. However, realistically, there will have to be a balance between the quality of the measurement and the practicality of surveillance.

  4. Primary Establishment of the Tissue Culture Technique and Regeneration System for Ornamental Lupinns polyphyllus

    Institute of Scientific and Technical Information of China (English)

    WANG Xiaoling; GAO Zhu; LIU Tengyun; YU Faxin; WANG Biqin

    2009-01-01

    The purpose of this paper is to develop a system for tissue culture and rapid propagation of two ornamental lupins, Minaretie and Russell Prize. In view of screening out the better explant regeneration and suitable culture medium, through adding hormone 6-B A, NAA and 2,4-D into MS and B5 basic culture medium, a series of experiments were carried out with the shoot tips, leaves, leaf petioles and stems from the asepsis seedling. The results showed that the shoot tips had favorableness on the rapidly propagation; MS+6-BA 0.5 rng·L-1 for first generation, the induction rate of Minaretie and Russell Prize was 90.5% and 95.86% respectivdy; Minaretie had the highest propagation index (6.35) on MS+6-BA 0.5 mg·L-1+NAA 0 mg·L-1+GA 30.8 mg·L-1+AC 2 g·L-1, but Russell Prize had the highest propagation index (7.24) on MS+6-BA 0.5 mg·L-1+NAA0.15 mg ·L-1+GA3 1.0 mg·L-1+AC 0.5 g·L-1; 1/2 MS+NAA 0.25 mg·L-1 was the best rooting medium. The ratios of getting roots of Minaretie and Russell Prize were 94.78% and 96.32%, respectively.

  5. Enhancing the corrosion resistance of dentistry drills by plasma immersion nitrogen in implantation of AISI 434-based SS

    Energy Technology Data Exchange (ETDEWEB)

    Munoz C, A.E.; Lopez C, R.; Fuentes G, D.; Valencia A, R. [UAEM, Facultad de Quimica, Instituto Literario 100, Toluca (Mexico)]. e-mail: rlc@nuclear.inin.mx

    2007-07-01

    In order to enhance the resistance to the pitting corrosion due to asepsis processes and to avoid structural fractures in dentistry drills, a plasma immersion ion implantation (PIII) treatment using nitrogen has been performed. The selected drill samples, made of AISI 434 based stainless steel with a 0.670 mm diameter, were treated at a -1kV bias between 350 C and 450 C, this temperature being controlled by both a 20-50 {mu}s pulse width and a 200-1000 Hz repetition rate in the bias. The drills were analysed by cyclic potentiodynamic tests showing a good pitting corrosion resistance when treated at around 400 C, as follows from a resulting very low hysteresis loop. Yet, the resistance appears somehow diminished by the presence of sputtering when processed at temperatures near 450 C. It is also found that the PIII nitriding effectiveness appears to be limited by the appearance of uniform corrosion. Finally, X-ray diffraction of the samples has revealed the presence of two new phases, namely {epsilon}-Fe{sub 2}N and Fe{sub 4.4}N, while scanning electron microscopy of the treated surfaces has suggested a sputtering enhancement at the highest temperatures. (Author)

  6. Applications of Midwives and Nurses Working at Pediatrics Clinics to Patients Security

    Directory of Open Access Journals (Sweden)

    Fatma Tas Arslan

    2014-06-01

    RESULT: The research 77.9% of them work as a nurse and %66.1 of them obtain information about patient security. In the applications to the patient security, and ldquo;I give the patients arm band and rdquo; constitutes 54. 1,% and ldquo;I have the concerned doctor sign the oral order form in 24 hours at the latest after it is filled and rdquo; constitutes 73.8%, and ldquo; I evaluate the patients in view of the risk of falling down and rdquo; constitutes 86.1%, and ldquo; I pay attention to asepsis in all invasive attempts and rdquo; constitutes 91% are among the regular ones. It was found out that among the nurses and midwives, 19 out of 103 made medical error during a year. CONCLUSiON: it has been achieved that nurses and midwives have different roles and pay attention to preventing the patients from falling down, providing the communication security, providing the infection security, providing the medicine security. It is thought that they should be supported in respect to identifying and confirming the patients ID [TAF Prev Med Bull 2014; 13(3.000: 209-216

  7. Relationship between hepatitis B and C virus prevalence and risk factors

    Directory of Open Access Journals (Sweden)

    Roxana POPESCU

    2009-11-01

    Full Text Available Hepatitis C virus (HCV and hepatitis B virus (HBV are major causes for liver disease worldwide and potential causes for substantial morbidity and mortality in the future. The aim of this study was to assess the prevalence of hepatitis C and B virus infection and to identify the associated risk factors of viral hepatitis. In our experiments we use as materials 107 patients, 48 men and 59 women, aged from 7 to 67 years old. The maximum incidence of HBV and HCV hepatitis was observed at patients aged between 40 to 60 and especially at people in urban areas. The number of cases with HCV was twice higher at men than at women, while for HVB the incidence was higher for men than for women. Risk factors for transmission of HCV and HBV were medical procedure in the high-risk group, while vertical or sexual transmission belongs to the low-risk patient group. In conclusion, this study demonstrates that the prevalence of HBV and HCV infection is high, thus asepsis and antisepsis measures should be strictly followed.

  8. New Conveyor Belt Lubrication Self-cleaning System%新型输送带润滑自清洁系统

    Institute of Scientific and Technical Information of China (English)

    王海兵; 许斌; 刘凤臣; 叶鹏

    2011-01-01

    In various types of beverage production line, conveyor belt plays an essential role. In order to prevent the bottle inverting, reducing wear, conveyor belt need to be lubricated. Lubrication by spraying is commonly used, but it exists kinds of problems such as non-uniform, waste, unfriendly-environment, breeding bacteria, scaling, and plugin noggle easily, difficult to clean and so on. Through comparative analysis, the paper proposed a new conveyor belt lubrication selfcleaning system with several advantages such as cycling lubrication, cleanness and asepsis, friendly environment conservation and maintenance fiee.[Ch,5 fig.1 tab.9 ref.]%在各类饮料生产线中,输送带起着不可或缺的作用.为了防止倒瓶、减少输送带自身的磨损,需对输送带进行润滑.通常采用的传统喷洒式润滑方法,在实际使用中存在润滑不均匀、浪费大、不环保、易长菌、易结垢、难清洁、喷嘴易堵塞等问题.通过对比分析,研制出一种新型的输送带滑润自清洁系统,具有循环润滑、清洁无菌、节约环保、免维护的优点.

  9. 浅谈阑尾炎手术切口感染的预防

    Institute of Scientific and Technical Information of China (English)

    曹翌

    2014-01-01

    Through the analysis of a large amount of clinical data of appendectomy and comparing of 200cases with emergency appendicitis we found some effective measurements of preventing the cut infections after appendectomy. It is very important to complete the folowing procedures: the early diagnosis to the emergent appendicitis patients, performing the surgery immediately, completely preparation before the surgery, choosing correct anesthesia, choosing the reasonable cuts, strictly asepsis performance, correct usage of antibiotics before and after the surgery, and the correct utility of drainage tubes.%本文通过大量阑尾手术资料的分析并对200例急性阑尾炎采取早诊断及时手术,采取充分做好术前准备、正确选择麻醉、选择合理的切口、术中严格执行无菌操作、手术前后合理应用抗生素、合理运用引流条等措施,通过对切口的观察,阐述了预防阑尾手术后切口感染的措施。

  10. Improvement of nursing students' learning outcomes through scenario-based skills training

    Science.gov (United States)

    Uysal, Nurcan

    2016-01-01

    Abstract Objective: this study analyzed the influence of scenario-based skills training on students' learning skills. Method: the author evaluated the nursing skills laboratory exam papers of 605 sophomores in nursing programs for seven years. The study determined the common mistakes of students and the laboratory work was designed in a scenario-based format. The effectiveness of this method was evaluated by assessing the number of errors the students committed and their achievement scores in laboratory examinations. This study presents the students' common mistakes in intramuscular and subcutaneous injection and their development of intravenous access skills, included in the nursing skills laboratory examination. Results: an analysis of the students' most common mistakes revealed that the most common was not following the principles of asepsis for all three skills (intramuscular, subcutaneous injection, intravenous access) in the first year of the scenario-based training. The students' exam achievement scores increased gradually, except in the fall semester of the academic year 2009-2010. The study found that the scenario-based skills training reduced students' common mistakes in examinations and enhanced their performance on exams. Conclusion: this method received a positive response from both students and instructors. The scenario-based training is available for use in addition to other skills training methods. PMID:27508922

  11. Factors affecting wound complications in head and neck surgery: A prospective study

    Directory of Open Access Journals (Sweden)

    Devendra A Chaukar

    2013-01-01

    Full Text Available Context: Head and neck surgeries are complex. Wound complications are associated with considerable morbidity and can result in delay in the adjuvant treatment. Identification of factors will help in formulating preventive guidelines. Aims: The aim of this study is to identify perioperative factors responsible for wound complications. Settings and Design: Prospective study of 186 head and neck patients. Subjects and Methods: Pre-operative, intraoperative and post-operative factors were recorded. Each patient was evaluated for minor and major wound complications twice daily during the hospital stay. Statistical Analysis: Chi-square test was used for univariate and log regression test was used for multivariate analysis. Results: The overall wound complication rate was 29% with 7% major and 22% minor complications. On univariate analysis, laryngeal and hypopharyngeal location, advanced T stage, poor oral hygiene, clean-contaminated surgery, low Karnofsky performance status (KPS, flap reconstruction, blood loss more than 1000 ml, perioperative blood transfusion, duration of surgery greater than 4.3 h and post-operative hemoglobin lesser than 11 g%, post-operative tracheostomy and resection of mandible were statistical significant factors. On multivariate analysis, post-operative tracheostomy, low KPS, post-operative serum albumin less than 3.7 g/dl and duration of surgery greater than 4.3 h were significant factors. Conclusion : Apart from unavoidable factors, it is essential to take care of certain factors viz nutrition, haemoglobin, oral hygiene, asepsis and repeating antibiotics in prolonged surgery.

  12. Social and practical representations in pneumonia associated to ventilation mechanics

    Directory of Open Access Journals (Sweden)

    Giovane Mendieta I

    2009-05-01

    Full Text Available Objective: To identify the practice and social representations of the respiratory therapists, on the strategies of prevention of pneumonia associated to mechanical ventilation, of the unit intensive care of the Kennedy Hospital. Methodology:Qualitative inquiry, descriptive and explanatory character, the population, respiratorys therapist of the unit’s intensives cares, with a sample of captive type for convenience, conformed by seventeen respiratorys therapists who were applied a semistructured interview, applying the content analysis technique for their study. Results: Practices and social representations, are given around the patient, and therefore avoid prolonged mechanical ventilation, the suction procedure should be carried out by means of aseptic technique, on the other and, the strict execution of handling protocols of airway; as long as it should be norm for the whole personnel, hand washing. Conclusions:it is observed the strategies of prevention are influenced by the theoretical references, however, these actors don’t know the full prevention strategies, likewise the represented practices are related with the prevention through asepsis transmission of pathogens, and has an effect on the construction of social representations and therefore the knowledge of new practices will change the structure of representation.

  13. Disinfection of Needleless Connector Hubs: Clinical Evidence Systematic Review

    Directory of Open Access Journals (Sweden)

    Nancy L. Moureau

    2015-01-01

    Full Text Available Background. Needleless connectors (NC are used on virtually all intravascular devices, providing an easy access point for infusion connection. Colonization of NC is considered the cause of 50% of postinsertion catheter-related infections. Breaks in aseptic technique, from failure to disinfect, result in contamination and subsequent biofilm formation within NC and catheters increasing the potential for infection of central and peripheral catheters. Methods. This systematic review evaluated 140 studies and 34 abstracts on NC disinfection practices, the impact of hub contamination on infection, and measures of education and compliance. Results. The greatest risk for contamination of the catheter after insertion is the NC with 33–45% contaminated, and compliance with disinfection as low as 10%. The optimal technique or disinfection time has not been identified, although scrubbing with 70% alcohol for 5–60 seconds is recommended. Studies have reported statistically significant results in infection reduction when passive alcohol disinfection caps are used (48–86% reduction. Clinical Implications. It is critical for healthcare facilities and clinicians to take responsibility for compliance with basic principles of asepsis compliance, to involve frontline staff in strategies, to facilitate education that promotes understanding of the consequences of failure, and to comply with the standard of care for hub disinfection.

  14. Trends of Various Techniques of Tubectomy: A Five Year Study in a Tertiary Institute

    Science.gov (United States)

    Prasanna, Naveen; Channabasappa, Ramalingappa Antartani

    2016-01-01

    Introduction Female sterilization is one of the best and effective methods of contraception for women who have completed their family. Tubectomy during caesarean operation and minilaparotomy are popular methods in developing countries whereas laparoscopic sterilization and hysteroscopic tubal occlusion are the preferred methods in developed countries. Aim To know the trends, incidence and immediate complications of methods of female sterilizations performed at our institute. Materials and Methods This is a retrospective analytical study conducted at our tertiary care centre from January 2010 to December 2014 in Karnataka Institute of Medical Sciences, Hubli, Karnataka. The case files of all the patients who underwent sterilization were taken from the medical records section and reviewed in detail. The cases were grouped as caesarean tubectomy, minilaparotomy and laparoscopic sterilization, based on the abdominal entry. For minilaparotomy and during caesarean tubectomy, modified pomeroy’s technique was used. For laparoscopic sterilization, falope rings were used. Data was analysed by Karl Pearson’s correlation co-efficient method and Chi-Square test. The p-value asepsis could be compromised when female sterilization is done in large numbers in camps. Hence target related approach towards female sterilization should be avoided. Laparoscopic sterilization has more procedure related complications, which can be better handled in tertiary care centres. PMID:26894125

  15. [Intra-articular injections].

    Science.gov (United States)

    Chapelle, Ch

    2015-09-01

    It is not unusual for a specialist or general practitioner to be presented with a pathology which necessitates the use of an intra-articular injection of corticosteroids, hyaluronic acid or a local anaesthetic. It would seem to be interesting to update and to precise the techniques and methods of intraarticular injections which have appeared in recent international publications, when we know that 30 % of the injections given into the knee and so called "dry" are incorrect and, therefore, inefficient. The indication of an articular injection depends, firstly, on the diagnosis which should be done with great care; after which should be an objective analysis complete with secondary effects linked to both the injection and the product used. The conditions of asepsis, the choice of needles and quantities of the injection and even the ways of the injections should be reviewed in detail. The last studies clearly question the secondary effects of the cartilage degradations of the cortisone given as an intra-articular injection and shows its efficiency on the pain and inflammatory phenomonen in osteoarthritis. Studies on hyaluronic acid are often contradictory going from a modest result to an important pain relief but it is necessary to be aware that the objective criteria are difficult to interpret. The use of local anaesthetics in intra-articular is limited by the few indications in view of the major risk of aggravating the pre-existing lesions by the disappearing signs of pain.

  16. 导尿管相关性泌尿系感染的研究%Clinical Analysis of Guide Catheter Associated Urinary System Infection

    Institute of Scientific and Technical Information of China (English)

    韩静

    2015-01-01

    Urethral catheterization and catheterization technique of diagnosis and nursing measures are the most common, due to dif iculty in urination and other reasons need retention catheterization, subject to the asepsis operating rules, as far as possible to shorten the catheter retention time, strengthening of routine maintenance of urinary catheter, improve the monitoring measures, the sterile principle runs through the whole process of indwel ing catheter prevention is an important measure guided catheter associated urinary tract infection.%导尿术及留置导尿是最常见的诊疗技术和护理措施,因排尿困难及其他原因需要保留导尿时,在遵循无菌操作规程前提下,尽可能缩短尿管保留时间,加强导尿管的日常维护,完善各项监测措施,将无菌原则贯穿保留尿管的全过程是预防发生导尿管相关性泌尿系感染的重要措施。

  17. Our experience in the management of infantile hydrocephalus: A study on thirty-five regrouped cases in Yaounde, Cameroon

    Directory of Open Access Journals (Sweden)

    F F Mouafo Tambo

    2011-01-01

    Full Text Available Background: Hydrocephalus is a frequent central nervous system disorder in children, and despite its importance, it has not been sufficiently studied in developing countries. Patients and Methods: A prospective and descriptive study on 35 cases of infantile hydrocephalus was carried out in the neurosurgery unit of the Yaounde Central Hospital, from March 2008 to January 2010. Results: The mean age of the patients was 6.69΁1.58 months, and the majority of them were in the 0-6 months age group (71.43%. The most frequent causes were congenital malformations, with stenosis of the aqueduct of Sylvius being the most represented (31.43%. As radiological workup, a CT scan was done in more than half of the cases (57.15%, and ventriculo-peritoneal shunting was the main surgical method of management used (94.29%. Infectious complications were observed in 22.86% of our cases. Conclusion: Hydrocephalus is a frequent disorder in this Cameroonian setting affecting mostly the 0-6months age group. For early diagnosis to be made, the head circumference of neonates should be routinely measured in the labour room and followed-up in all medical visits. To avert complications following surgery, rigorous surgical procedures with effective asepsis and appropriate methods and materials for shunting should be used.

  18. Variation of Serum Levels of Glucose and Protein During the Day in Sheep of Different Sex

    Directory of Open Access Journals (Sweden)

    Carmen Galván Doria

    2014-07-01

    Full Text Available In order to establish the effect of sampling hour and sex on blood glucose levels and total protein, there were monitored six Colombian native sheep for 3 days. The blood samples were taken by jugular venipuncture, after asepsis and stored at -20°C to its subsequent processing through Spectrophotometry. The results were tabulated in Excel and analyzed using statistical program SAS 9.1. The mean values found were: glucose 75.57 ± 27.5 mg/dL for females and 83.70 ± 37.7 mg/dL for males; total protein 8.47 ± 2.76 g/dL for females and 7.14 ± 3.26 g/dL for males; albumin 4.29 ± 1.41 g/dL for females and 3.83 ± 1.46 g/dL for males; globulin 4.62 ± 2.35 g/dL for females and 3.92 ± 2.45 g/dL for males. The day and the hour of sampling affect serum concentrations (p < 0.05 of evaluated metabolites, also there was variation of themself regarding sex (p < 0.05.

  19. A simple technique for the determination of aseptic inoculation room cleanliness%无菌接种室洁净度测定简易技术

    Institute of Scientific and Technical Information of China (English)

    何金柱; 郎凤红; 杨贵荷; 任彦青

    2014-01-01

    This paper reports a method for the determination of super clean sterile chamber and worktable for the wine grape stem tip culture, detoxification and rapid propagation in Ningxia Yuquanying. According to the requirements of determining the number of bacteria count and necessary measures, the cleanliness of asepsis inoculating room can be obtained. The cleanliness standards are discussion too. This study will offer a reference for detoxification and rapid propagation in desert area.%总结在宁夏玉泉营进行酿酒葡萄茎尖培养、脱毒和无菌快繁建立无菌接种室和超净工作台洁净度的测定方法,按要求进行降落菌数测定计数和必要的措施等,能获得符合无菌接种室洁净度,并对洁净度的标准进行讨论,为沙区进行脱毒和快繁提供参考。

  20. The discuss on quality control methods of culture medium for thermotolerant coliform bacteria%检测耐热大肠菌群培养基的质量控制方法探讨

    Institute of Scientific and Technical Information of China (English)

    钟宁

    2012-01-01

      The quality of culture medium is the foundation of the microbial detection, affecting directly the reliability of the test results. In this paper, combining with the actual situation, the discuss on quality control of the EC culture medium for thermotolerant coliform bacteria was carried out through the outside,pH value, asepsis check, sensitivity check and others aspects so as to microbiology laboratory perfect and improve continuously culture medium quality level , and to ensure the accuracy of the test results.%  培养基的质量是微生物检测工作的基础,直接影响检测结果的可靠性。本文结合实际情况,从外观、pH值、无菌检查及灵敏度检查等几个方面,对检测耐热大肠菌群所用EC培养基的质量控制进行讨论,以便微生物实验室不断完善和提高培养基的质控水平,从而保证检验结果的准确性。

  1. Evaluation of microbiocidal activity of superoxidized water on hospital isolates

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    Aggarwal Ritu

    2010-10-01

    Full Text Available Background: Prevention of nosocomial infections, pre-operative and post-operative complications is directly linked with effective disinfection and decontamination. Microbial decontamination is the most serious challenge to the today′s health care practice despite the abundance of disinfectants and chemicals as there are increasing reports of emergence of resistance to the action of commonly used disinfectants. There is a need to evaluate the efficacy of newer methods of asepsis for better patient management. Aim: This study was designed to evaluate the microbiocidal activity of superoxidized water (SOW on common clinical isolates, ATCC strains, vegetative cells and spores of Bacillus subtilis. Materials and Methods: Bacterial suspensions were treated with SOW and deionized water (control. All the tubes were incubated at 37°C for 0.5, 2.5 and 5.0 min. The number of viable cells was counted. Results: All the clinical isolates and ATCC strains were killed within 0.5 min of exposure to the SOW. Vegetative cells and spores of B. subtilis were killed after 5.0 min. Conclusion: We conclude that SOW is an effective microbiocidal agent for routine hospital use.

  2. 浅谈阑尾炎手术切口感染的预防

    Institute of Scientific and Technical Information of China (English)

    曹翌

    2013-01-01

    本文通过大量阑尾手术资料的分析并对200例急性阑尾炎采取早诊断及时手术,采取充分做好术前准备、正确选择麻醉、选择合理的切口、术中严格执行无菌操作、手术前后合理应用抗生素、合理运用引流条等措施,通过对切口的观察,阐述了预防阑尾手术后切口感染的措施。%Through the analysis of a large amount of clinical data of appendectomy and comparing of 200cases with emergency appendicitis we found some effective measurements of preventing the cut infections after appendectomy. It is very important to complete the fol owing procedures: the early diagnosis to the emergent appendicitis patients, performing the surgery immediately, completely preparation before the surgery, choosing correct anesthesia, choosing the reasonable cuts, strictly asepsis performance, correct usage of antibiotics before and after the surgery, and the correct utility of drainage tubes.

  3. Microbial air monitoring in the operating theatres of Salam Center for Cardiac Surgery in Khartoum (Sudan

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    Margherita Scapaticci

    2012-06-01

    Full Text Available The seriousness of postoperative infections and the increased susceptibility of patients undergoing cardiac surgery increase the demand for the operating theatre (OT asepsis to prevent bacterial infections. In fact, the organisms carried by the air reach the wound after having sedimented onto sterile field. The air represents a critical point for quality control of air filtration systems, for sanitization procedures and for the evolution of hygienic features of the OT environment.Aim of the study is to evaluate the prevalence of microorganisms found in the operating rooms (OR air monitoring in the Salam Center for Cardiac Surgery of Khartoum (Sudan between July 2008 and March 2009.The specimens were collected every month in two different times: “OR at rest” (after sanitization and “OR operational”, using sedimentation method (Fisher 1972. Results showed that each sample collected at rest had IMA (index of microbial air contamination < 5CFU/plt, whereas the bacterial growth was between 25 and 50 CFU/plt when the samples had been collected in the same places during operating activities.This indicate the effectiveness of sanitization procedures and confirm that people working in OT are an important source of bacteria causing postoperative infections. Coagulase negative Staphylococci, Gram negative bacillus and Staphylococcus aureus spp. were the predominant organisms isolated.

  4. Clinical Application of Paper-plastic Package's Extension of Autoclave Pack%纸塑包装延长高压灭菌包的临床应用

    Institute of Scientific and Technical Information of China (English)

    冉成华

    2011-01-01

    目的:纸塑包装延长高压灭菌包的临床应用.方法:双层棉包布常规包装高压灭菌按要求存放7d,霉季5d,而纸塑包装可保存6个月.结果:纸塑包装大大延长无菌包的无菌时间.包装安全经济,可靠,并可预防院内感染.%Goal: clinical application of paper-plastic package’s extension of autoclave pack.Method: things regularly packed with double cotton and autoclaving can be stored for 7d, or 5d in mildew season; but thing packed with paper can be stored for 6 months.Result: paper pack greatly expands the asepsis time of sterility pack.The pack is safe and reliable, and can prevent infection in hospital.

  5. Performance analysis of exam gloves used for aseptic rodent surgery.

    Science.gov (United States)

    LeMoine, Dana M; Bergdall, Valerie K; Freed, Carrie

    2015-05-01

    Aseptic technique includes the use of sterile surgical gloves for survival surgeries in rodents to minimize the incidence of infections. Exam gloves are much less expensive than are surgical gloves and may represent a cost-effective, readily available option for use in rodent surgery. This study examined the effectiveness of surface disinfection of exam gloves with 70% isopropyl alcohol or a solution of hydrogen peroxide and peracetic acid (HP-PA) in reducing bacterial contamination. Performance levels for asepsis were met when gloves were negative for bacterial contamination after surface disinfection and sham 'exertion' activity. According to these criteria, 94% of HP-PA-disinfected gloves passed, compared with 47% of alcohol-disinfected gloves. In addition, the effect of autoclaving on the integrity of exam gloves was examined, given that autoclaving is another readily available option for aseptic preparation. Performance criteria for glove integrity after autoclaving consisted of: the ability to don the gloves followed by successful simulation of wound closure and completion of stretch tests without tearing or observable defects. Using this criteria, 98% of autoclaved nitrile exam gloves and 76% of autoclaved latex exam gloves met performance expectations compared with the performance of standard surgical gloves (88% nitrile, 100% latex). The results of this study support the use of HP-PA-disinfected latex and nitrile exam gloves or autoclaved nitrile exam gloves as viable cost-effective alternatives to sterile surgical gloves for rodent surgeries.

  6. Evaluation of Tetanus Vacination Status of Pregnant Mothers Referring to Delivery Wards, Yazd

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    B Enjezab

    2005-07-01

    Full Text Available Introduction: Tetanus is a serious infectious disease and the most common cause of neonatal mortality in developing countries. Although neonatal tetanus (NT can be prevented by immunization of expectant mothers, good hygiene and asepsis during delivery, total eradication of organism is not possible. Methods: The cross-sectional study was done on 480 pregnant women after delivery. Data was collected by a questionnaire and analysed using SPSS windows software program. Results: Prenatal immunization rate (complete and partial was 65/2% and 34/8%, respectively. There was a significant relationship between the vaccination status and age, education of mother, parity and length of time between pregnancy and previous pregnancy. The reasons reported by non immunized mothers (not receiving the two dose vaccine included complete tetanus vaccination coverage before pregnancy, lack of awareness about the needs for vaccination, improper counseling by persons responsible for antenatal care, e.t.c. Conclusion: Considering the results of the study, it is proposed that a complete history of the pregnant woman recorded at the first antenatal visit should be the basis of counseling and education of the woman and prenatal care staff should be also be fully educated.

  7. Central venous catheter-related infections: Risk factors and effects of glycopeptide antibiotics

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    Arsenijević Ljubica

    2007-01-01

    Full Text Available INTRODUCTION Central venous catheters (CVC are used in the treatment of critically ill patients. Indications for placement of CVCs include hemodynamic monitoring, administration of intravenous fluids, medications and total parenteral nutrition. MATERIAL AND METHODS We investigated risk factors and effects of glycopeptide antibiotics on the development of central venous catheter-related infections in 300 patients treated in intensive care units. A semiquntitative culture technique was used. The investigation included: age, diagnosis on admission, catheter insertion site, catheter duration, the first or next catheter and using of glycopeptide drugs. RESULTS 91 catheters (30.3% were colonised, catheter-related infection was found in 50 catheters (16.7%. Infections were more frequent in catheters inserted through the internal jugular vein than in subclavian venous catheters; they were also more frequent if duration of catheterization was longer than seven days, but less frequent in patients who received glycopeptide antibiotics. The isolated microorganism was Staphylococcus aureus. DISCUSSION According to the literature, a number of catheter-related risk factors for infections include: insertion site, type of catheter, the number of manipulations, inadequat asepsis, lumen number, type of antiseptic. The relative importance of one risk factor over another is difficult to assess, given that studies have no priority report. CONCLUSION The duration of catheterization and the insertion site were the most frequent risk factors for infection. The use of glycopeptide antibiotics during catheterization has protective effects.

  8. Is fumigation enough for air conditioning units in operation theatres and Intensive care units?

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    Anasua Deb

    2016-05-01

    Full Text Available Background: Strict asepsis is necessary in operating theatres (OT and intensive care units (ICU as the patients undergo invasive procedures. The filters of contaminated air conditioning (AC units provide a niche for proliferation of fungi and production of fungal spores. Methods: The routine procedure for maintenance of sterile atmosphere in our hospital, i.e. fumigation and mopping walls with disinfectants often fail to address these fungal spores of the AC filters. We therefore carried out a surveillance of the ACs in ICUs and OTs to find the level of contamination with fungal spores and also to improvise on intervention strategies to tackle the problem. Over 3 months period, 34 ACs from 7 OTs and 2 ICUs were screened by taking 2 swabs from each AC which were then tested for the presence of fungal spores as per standard methods. Results: The contamination rate was 88.2% before fumigation and 76.9% after fumigation. The fungal spore contamination rate was reduced to 20% (1 out of 5 ACs after servicing of the ACs was done. Aspergillus spp. was the most common fungal isolate. Conclusion: Based on the observations, we recommend regular servicing of the ACs as well as wet mopping of the ducts with sporicidal solution at regular intervals. [Int J Res Med Sci 2016; 4(5.000: 1583-1589

  9. Fungos associados às sementes de ipê-amarelo (Tabebuia serratifolia e ipê-roxo (Tabebuia impetiginosa: incidência, efeito na germinação e transmissão para as plântulas Fungi associated to the seeds of ipê-amarelo (Tabebuia serratifolia and ipê-roxo (Tabebuia impetiginosa: incidence, germination effect and seedlings transmission

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    Luana da Silva Botelho

    2008-12-01

    (Tabebuia serratifola and ipê-roxo (T. impetiginosa collected from the region of Piracicaba, Mogi-Guaçu and south of Minas Gerais (Lavras, Ijaci e Itumirim and to determine the possible damages in the seedlings production of those species. The method used for the health test was the blotter test and, for the germination, gerbox had been used with paper substratum at temperature of 30ºC under constant light regime. The seeds, as much in the health test as in the germination, tests were sub sampled in one part that was submitted to superficial desinfestation with sodium hypochlorite of, and one part that wasn't. The fungi transmission had been evaluated through lesions found in the seedlings, during the germination test. Sixteen fungi had been identified and quantified: Cladosporium sp., Alternaria alternata, Epicoccum sp., Phoma sp., Geotrichum sp., Penicillium sp., Trichothecium sp., Phomopsis sp., Drechslera sp., Aspergillus spp., Curvularia sp., Fusarium spp., Macrophomina phaseolina, Nigrospora sp., Lasiodiplodia theobroma and Septoria sp. In general , the asepsis provided a drastic reduction in the incidence of all fungi, in both species, with an avarege rate of 90%. Like this, it can be inferred that most of the fungi was contaminating the seeds. The percentage of normal seedlings had not been interfered directly by the fungi. The asepsis reduced the germination in 64%, indicatinwas fitotoxic. In the transmission it had been observed, on average, 17% and 10% of seedlings with symptoms, in the samples without asepsis and with asepsis, respectively. The most frequent fungi transmitted by the ipê-amarelo and ipê-roxo seeds had been: Alternaria alternata, Fusarium spp., Aspergillus spp., Phoma sp. and Phomopsis sp.

  10. Modern pacemaker therapy

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    Mijatov Milan

    2010-01-01

    Full Text Available Introduction. Pacemakers are devices that modern medicine and cardiology cannot be imagined without. The technique of implantation comes to surgical procedure where all principles of asepsis and antisepsis have to be respected. Although some complications do happen, they are rather rare. Results. After the implantation of the device, the patient is not handicapped (unless the heart was additionally damaged. On the contrary, the patient returns to his work and functions normally within his family in most of the cases. The first medical appointment is scheduled a month after the implantation and the following are three and six months after. Types of devices. Today there are „new types of electrostimulation”- implantable cardioverter defibrillators and multisite electrostimulators. The former is implanted in patients at high risk of sudden cardiac death and the latter in patients with heart failure and left bundle branch block. Owing to these devices, the sudden cardiac death can be prevented successfully and the quality of a patient’s life is improved.

  11. Knowledge of aseptics and antisepsis and following their rules as elements of infection prevention in the work of paramedics

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    Łukasz Szarpak

    2013-04-01

    Full Text Available Background: The health-care professionals, particularly paramedics are exposed to bloodborne infections during medical rescue operations. The aim of this study was to assess knowledge of bloodborne infections, their prevention and interrupted routes of infection transmission among paramedics. Materials and Methods: The study comprised 220 paramedics employed in emergency departments and ambulance services. Material was collected using an anonymus questionnaire, developed for the purpose of this study. Results: As many as 80% of the surveyed people reported frequent contact with patients' blood; 65% of the paramedics before establishing the intravenous access palpates the conduct of the vein; 81% introduce peripheral venous catheter directly after disinfection of the skin; and 98% apply personal protection measures. Conclusions: The analysis of knowledge of asepsis and antisepsis of bloodborne infections among paramedics points to a series of shortcomings in their adequate preparation to reduce the effects of exposure to infectious material. However, it should be emphasized that people with higher education presented a higher level of knowledge than those with postsecondary vocational education. In the opinion of the study group mandatory training in the discussed area would prove to be the most effective method for reducing the number of bloodborne infections. Med Pr 2013;64(2:239–243

  12. Tissue Culture and Rapid Multiplication Techniques of Apocynum L.

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] This study aimed to investigate rapid multiplication of Apocynum by tissue culture so as to provide plantlet sources for its industrialized cultivation. [Method] The asepsis seedlings were obtained by dealing with Apocynum seeds. Its cotyledons, hypocotyls and shoot tips were cultured on the media containing different concentrations of hormones. Finally, the influence of different hormone combinations on differentiation of cotyledons and hypocotyls, rapid multiplication of shoot tips, rapid multiplication of regenerated shoots, and rooting of test-tube plantlets was com- pared. [Result] MS+2.0 mg/L BA+0.03 mg/L NAA and MS+0.07 mg/L NAA were the optimum medium for inducing regenerated buds from cotyledons and hypocotyls re- spectively; MS+2.0 mg/L BA+0.02 mg/L NAA was the best medium for rapid multi- plication of shoot tips; MS+1.9 mg/L BA+I.7 mg/L NAA was the best medium for rapid multiplication of regenerated buds: and 1/2MS+0.6 mg/L NAA was the best medium for inducing roots. [Conclusion] The optimum hormone combination was de- termined for Apocynum rapid multiplication by tissue culture, which provides technical support on Apocynum industrialized cultivation.

  13. SURGICAL SITE INFECTION IN ABDOMINAL SURGERIES: A CLINICAL STUDY

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    Amit

    2014-09-01

    Full Text Available Purpose: A prospective study of incisional surgical site infection in abdominal surgeries was conducted to find out incidence, common predisposing factors and microbiological profile. METHODS: 375 patients who underwent abdominal surgeries were studied in a span of 1 year. They were divided into two groups – group 1 – 316 cases who did not develop Surgical Site Infection (SSI, the 2nd group – 59 cases that developed SSI. These patients were studied by interviewing and examination according to a set proforma. The results were statistically analyzed by comparing averages using Chi square chart for finding significance of difference where applicable. RESULT: The overall SSI incidence was 15.7 % (59/375. In elective surgeries, the SSI rate was 5.7% and in emergency surgeries, it was 28.6%. It was found that SSI increased with increasing age linearly. Other significant factors involved were increasing class of wound (dirty > clean wound class, increased preoperative stay, presence of remote site infection, increased duration of surgery and use of drains. E. coli was found to be the most common organism causing SSI in abdominal operations. CONCLUSION: SSI can be reduced by decreasing the preoperative hospital stay, appropriate antibiotic administration policies, preoperative control of remote site infections, adequate preoperative patient preparation, reducing the duration of surgery to minimum, judicious use of drains and intraoperative maintenance of asepsis and following operation theatre discipline properly.

  14. Peritoneal dialysis peritonitis: risk factors and effects

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    Alikari V.

    2013-07-01

    Full Text Available Introduction: Peritonitis is the most frequent complication of peritoneal dialysis. It is the main cause of failure of the method and inclusion of patients in hemodialysis Purpose: The purpose of this study is to explore the risk factors of peritoneal dialysis peritonitis as well as the acute and long-term effects of peritonitis on peritoneal membrane function and success of the method. Methods: Literature review of relevant articles in the database Pubmed as well as the official website of the International Society of Peritoneal Dialysis (ISPD was carried out. The search included articles published during the period 1986 -2012.Results: Lack of patient compliance with the peritoneal changes protocol, lack of social support, systemic diseases and connection systems are risk factors of peritonitis. Peritonitis causes transient increase of peritoneum permeability, loss of the ability of the peritoneum for ultrafiltration and, consequently, overhydration. Long-term, recurrent episodes of peritonitis resulting in permanent loss of peritoneum capacity for ultrafiltration even fibrosis that leads to failure of the method. Finally, its effect on the reduction of the residual renal function seems to be remarkable. Conclusions: Peritonitis is the most common infectious complication of peritoneal dialysis and the main cause of morbidity associated with the method. Moreover, it is the major cause of failure of the method and patients accession in hemodialysis. Any attempt to prevent peritonitis passes through the careful patient selection and effective patient education on compliance with the rules of asepsis during peritoneal changes.

  15. In vitro cultivation of zygotic embryos from Murici (Byrsonima cydoniifolia A. Juss.: establishment, disinfection, and germination - doi: 10.4025/actasciagron.v35i2.15402

    Directory of Open Access Journals (Sweden)

    Cíntia de Oliveira Martendal

    2012-12-01

    Full Text Available The objective of this study is to establish an in vitro germination and cultivation protocol for murici (Byrsonima cydoniifolia A. Juss. using zygotic embryos. Therefore, three assays were performed: in assay I, embryo asepsis was tested at exposure times of 5, 10, 15, 20, 25, and 30 minutes in 2.5% sodium hypochlorite, with or without immersion in 70% alcohol; in assay II, MS (MURASHIGE; SKOOG, 1962 e WPM (LLOYD; McCOWN, 1980 culture media were tested at salt concentrations of 25, 50, and 100%, with or without the addition of sucrose, to germinate the buds; in assay III, seedling growth was evaluated in MS and WPM culture media at salt concentrations of 25, 50 and 100%. Sodium hypochlorite (2.5% with or without 70% alcohol was used to avoid contamination because it was not toxic to murici embryos. Water-agar was the most appropriate culture medium for bud germination, and 50% WPM was appropriate for seedling growth.

  16. Antoni Christian Bryk (1820-1881) - professor of forensic medicine at Jagiellonian University (1852-1860), and director of the surgical clinic of Jagiellonian University in Cracow (1860-1881).

    Science.gov (United States)

    Zajączkowski, Tadeusz

    2014-01-01

    scientific discipline. In the 19th century two milestones revolutionised surgery: the development of narcosis, which enabled painless surgery, and the introduction of antisepsis and asepsis. The author presents the beginnings of academic surgery in Cracow. Its pioneer surgeons are presented. Extensive research was undertaken to collect the literature and documents in Polish, Austrian and German archives and libraries in order to prepare this study. Biographical details of the director of the Surgical Clinic, Prof. Antoni Bryk, are provided. He was the first person in Poland to introduce antisepsis and galvanocautery as routine procedures in the Cracow Clinic. The introduction of antisepsis contributed to a reduction in infection during surgery, and a reduction in postoperative mortality in Cracow Surgical Clinic. In this way Professor Bryk became the first Polish surgeon to apply Lister's antiseptic method in the treatment of wounds. Thus enlarged, the scope of surgery for intracranial, bone and other procedures became routine. Surgery is the oldest discipline in medicine. Poland's first university chair of surgery was established in the 18th century. Surgery, which until then had been the domain of barbers and bath house attendants, became a clinical, PMID:25518102

  17. Proximal femur fractures: the pioneer era of 1818 to 1925.

    Science.gov (United States)

    Bartonícek, Jan

    2004-02-01

    The foundations of the modern era of the treatment of proximal femur fractures were laid by Cooper who in 1819 published the first therapeutically oriented classification of these fractures. During the following 100 years the clinical diagnostics were further developed; five classifications of proximal femur fractures were formulated including the terminology used until now (peritrochanteric, intertrochanteric), and surgical treatment was started. The first attempt at internal fixation of the proximal femur in the 1850s was by Langenbeck. His patient died of sepsis. The first successful internal fixation of proximal femur fractures was done in 1875 by König. At the beginning of the twentieth century the method of surgical treatment for proximal femur fractures was improved by Lambotte, Delbet, and Putti. However, the time was not ready yet for this method of treatment. Only technologic progress (stainless steel, new technology), the additional development of anesthesia and asepsis, improvement of xray apparatus, and introduction of the traction table created the prerequisites for successful gradation of Smith-Petersen's method from 1925 to 1931, and which a few years later was used worldwide. PMID:15021169

  18. Enhancing the corrosion resistance of dentistry drills by plasma immersion nitrogen in implantation of AISI 434-based SS

    International Nuclear Information System (INIS)

    In order to enhance the resistance to the pitting corrosion due to asepsis processes and to avoid structural fractures in dentistry drills, a plasma immersion ion implantation (PIII) treatment using nitrogen has been performed. The selected drill samples, made of AISI 434 based stainless steel with a 0.670 mm diameter, were treated at a -1kV bias between 350 C and 450 C, this temperature being controlled by both a 20-50 μs pulse width and a 200-1000 Hz repetition rate in the bias. The drills were analysed by cyclic potentiodynamic tests showing a good pitting corrosion resistance when treated at around 400 C, as follows from a resulting very low hysteresis loop. Yet, the resistance appears somehow diminished by the presence of sputtering when processed at temperatures near 450 C. It is also found that the PIII nitriding effectiveness appears to be limited by the appearance of uniform corrosion. Finally, X-ray diffraction of the samples has revealed the presence of two new phases, namely ε-Fe2N and Fe4.4N, while scanning electron microscopy of the treated surfaces has suggested a sputtering enhancement at the highest temperatures. (Author)

  19. 无高危因素的三种清洁手术预防用药%Preventive medication in three kinds of clean surgery without high-risk factors

    Institute of Scientific and Technical Information of China (English)

    庞晓军; 赖广平; 邓忠南; 叶开华; 朱其海

    2011-01-01

    目的:探讨无高危因素的三种清洁手术是否需要预防用药.方法:选择2009年7月- 2011年4月在我院住院需进行乳腺、疝气、甲状腺手术无高危因素患者63例,采用随机数字表随机分为两组,试验组在严格的无菌操作条件下进行手术治疗,仅采用聚维酮溶液消毒,对照组手术前30 min给予五水头孢唑林1~2 g,静脉滴注,两组遵从严格的无菌操作要求进行手术,对比两组术后体温恢复正常时间、切口感染率、住院药费及术后住院时间.结果:两组术后体温恢复时间、切口感染率和术后住院时间无统计学差异,试验组住院药费明显低于对照组.结论:无菌手术切口感染与预防性使用抗菌药物无关,无高危因素清洁手术我们不推荐常规给予抗菌药物预防感染.%OBJECTIVE To discuss the topic of preventive medication in three kinds of clean surgery without high-risk factors. METHODS 63 cases of hospitalized patients were chosen in July, 2009 - April, 2011 for carrying on breast, hernia and thyroid operation without high-risk factors in our hospital. By using table of random number, the patients were divided into two groups stochastically. The patients of experimental group were treated with the surgery under the strict asepsis operating condition, only pvp-iodine solution was used for disinfection. In the control group 30min before surgery the patients were given with cefazolin sodium pentahydrate 1-2 g, iv drip. Both groups complied with the strict asepsis operation to carry on the surgery. The time of body temperature to restore to the normal level, the infection rate of incisional wound, the charges in hospital for medicine and the hospitalized days were compared between the two groups. RESULTS There was no statistic difference in the time of body temperature to restore to the normal level, the infection rate of incisional wound and the hospitalized days between the two groups. The charges in hospital for

  20. Conservação e enraizamento in vitro de infalível (Mandevilla velutina K. Schum., uma planta medicinal do Cerrado In vitro conservation and rooting of "infalível" (Mandevilla velutina K. Schum., a medicinal plant of Cerrado

    Directory of Open Access Journals (Sweden)

    A.V. Souza

    2011-01-01

    , dithiothreitol and phloroglucinol. Evaluations were performed at 30 and 60 days as to rooting percentage, and root number and length. For the introduction of genotypes in vitro, nodal segments (1 cm were used as explants; they had an axillary or apical bud and were collected from plants kept in a greenhouse after being subjected to asepsis. Evaluations were carried out for four weeks as to the percentage of explant contamination. Results showed that the presence of phenolic compounds in the culture medium was important to promote in vitro adventitious rooting in M. velutina and that the asepsis methodology for the introduction of in vitro of different genotypes was efficient.

  1. Maternal mortality -- aetiological factors: analytic study from a teaching hospital of Punjab.

    Science.gov (United States)

    Sarin, A R; Singla, P; Kaur, H

    1992-01-01

    A review of maternal deaths at Rajendra Hospital, Punjab, from January 1978 to December 1991 yielded important data for the planning of maternal health services in this area of India, During the 14 year study period, there were 33,160 births and 339 deaths, for a maternal mortality rate of 1002/100,000 live births. Women who had received no prenatal care accounted for 47.4% of deliveries but 92.8% of maternal deaths. In addition, a disproportionate number of deaths involved rural women (74.6%) and poor women (76.4%). 57.8% of maternal deaths involved women 21-30 years of age; 37.1% occurred among primigravidas. Direct obstetrical causes were considered the etiologic factor in 83.1% of these deaths. Primary among these causes were sepsis (37.1%), obstetric hemorrhage (26.2%), hypertensive disorders of pregnancy (21.4%), and obstructed labor (15.3%). 30.6% of deaths occurred during pregnancy, 50.3% during labor, and 19.1% in the postpartum period. Indirect obstetrical causes, notably severe anemia and anesthesia complications, were implicated in 15.3% of the maternal deaths. Critical analysis of the maternal deaths in this series suggested that 89.6% were totally preventable, 9.6% were probably preventable, and only 0.8% were not avoidable. Factors that would reduce the high rate of maternal mortality in this region include more widespread use of prenatal care, training of traditional birth attendants in asepsis, referral of high-risk pregnancies, and improved transportation in rural areas. PMID:12288813

  2. Reguladores vegetais e teores endógenos de poliaminas durante o desenvolvimento de taro cultivado in vitro Plant growth regulators in polyamines endogenous levels during the development of taro cultivated in vitro

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    Adriana Alice Francisco

    2008-08-01

    Full Text Available Neste trabalho foi investigada a assepsia para obtenção de explantes oriundos de tubérculos e a ação das poliaminas espermidina e espermina exógenas associadas aos reguladores vegetais AIA e BA no desenvolvimento, na tuberização in vitro e nos níveis endógenos de putrescina (Put, espermidina (Spd e espermina (Spm de taro (Colocasia esculenta. Plantas crescidas em meio contendo espermidina e espermina mostraram tuberização e a associação dessas poliaminas com AIA e BA induziu aumento do número de brotações. Para o estímulo da rizogênese, não foi necessário o uso de reguladores vegetais. Altos teores de putrescina foram encontrados durante a emissão de brotações, enquanto que altos teores de espermidina foram observados durante a formação de rizomas in vitro.The present research investigated the asepsis for attainment of explants deriving of tubers and the polyamines espermidine and espermine exogenous effects associated with the plant growth regulators NAA and BA on the development and tuberization in vitro and the endogenous levels of putrescine (Put, spermidine (Spd and spermine (Spm of taro (Colocasia esculenta. Plants grown in the medium with spermidine and spermine showed tuberization and the association of these polyamines with NAA and BA increases number of shoots. Plant growth regulators were not necessary for root initiation. High levels of endogenous Put were found during the shoot emission, while high levels Spd were observed during in vitro root formation.

  3. Commercial considerations in tissue engineering.

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    Mansbridge, Jonathan

    2006-10-01

    Tissue engineering is a field with immense promise. Using the example of an early tissue-engineered skin implant, Dermagraft, factors involved in the successful commercial development of devices of this type are explored. Tissue engineering has to strike a balance between tissue culture, which is a resource-intensive activity, and business considerations that are concerned with minimizing cost and maximizing customer convenience. Bioreactor design takes place in a highly regulated environment, so factors to be incorporated into the concept include not only tissue culture considerations but also matters related to asepsis, scaleup, automation and ease of use by the final customer. Dermagraft is an allogeneic tissue. Stasis preservation, in this case cryopreservation, is essential in allogeneic tissue engineering, allowing sterility testing, inventory control and, in the case of Dermagraft, a cellular stress that may be important for hormesis following implantation. Although the use of allogeneic cells provides advantages in manufacturing under suitable conditions, it raises the spectre of immunological rejection. Such rejection has not been experienced with Dermagraft. Possible reasons for this and the vision of further application of allogeneic tissues are important considerations in future tissue-engineered cellular devices. This review illustrates approaches that indicate some of the criteria that may provide a basis for further developments. Marketing is a further requirement for success, which entails understanding of the mechanism of action of the procedure, and is illustrated for Dermagraft. The success of a tissue-engineered product is dependent on many interacting operations, some discussed here, each of which must be performed simultaneously and well.

  4. Commercial considerations in tissue engineering.

    Science.gov (United States)

    Mansbridge, Jonathan

    2006-10-01

    Tissue engineering is a field with immense promise. Using the example of an early tissue-engineered skin implant, Dermagraft, factors involved in the successful commercial development of devices of this type are explored. Tissue engineering has to strike a balance between tissue culture, which is a resource-intensive activity, and business considerations that are concerned with minimizing cost and maximizing customer convenience. Bioreactor design takes place in a highly regulated environment, so factors to be incorporated into the concept include not only tissue culture considerations but also matters related to asepsis, scaleup, automation and ease of use by the final customer. Dermagraft is an allogeneic tissue. Stasis preservation, in this case cryopreservation, is essential in allogeneic tissue engineering, allowing sterility testing, inventory control and, in the case of Dermagraft, a cellular stress that may be important for hormesis following implantation. Although the use of allogeneic cells provides advantages in manufacturing under suitable conditions, it raises the spectre of immunological rejection. Such rejection has not been experienced with Dermagraft. Possible reasons for this and the vision of further application of allogeneic tissues are important considerations in future tissue-engineered cellular devices. This review illustrates approaches that indicate some of the criteria that may provide a basis for further developments. Marketing is a further requirement for success, which entails understanding of the mechanism of action of the procedure, and is illustrated for Dermagraft. The success of a tissue-engineered product is dependent on many interacting operations, some discussed here, each of which must be performed simultaneously and well. PMID:17005024

  5. The prevention and management of hospital infection in the stomatological consulting room%口腔科诊室医院感染的预防与管理

    Institute of Scientific and Technical Information of China (English)

    郭李柯

    2011-01-01

    目的 加强口腔科诊室医院感染的控制与管理,降低医院感染的发生率。方法 针对口腔科诊室的工作特点,制定并落实各项有效的消毒隔离措施和感染监测制度,严格执行无菌操作规程,创造合格的手术环境。结果 无菌切口感染率为0.19%,特殊感染手术无医院感染发生。结论 加强口腔科诊室医院感染的管理,可有效地防止医院感染的发生,降低无菌切口感染率。%Objective To strengthen the administration the the hospital infection control in the stomatological consulting room for decreasing the incidence rate of the hospital infection.Methods According to the working characteristics of the stomatological consulting room, we enacted and implemented the effective measures of disinfection, isolation and infection monitoring institution, strictly implemented the asepsis operating rules, and created a valid operation environment.Results The infection rate of the incisional wound was 0.19%.The hospital infection was not happened in the special infected operation.Conclusions To strengthen the administration of the hospital infection control in the stomatological consulting room can effectively prevent the hospital infection and reduce the infection rate of the incisional wound.

  6. Cross-contamination in dentistry: A comprehensive overview

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    Sagar J Abichandani

    2013-01-01

    Full Text Available Introduction: Cross-contamination and cross-infection can occur by direct contact with micro-organisms, indirect contact with contaminated objects, droplet transmission, and inhalation of airborne pathogens. In dentistry, operatory surfaces can routinely become contaminated with patient saliva, blood, and other fluids during treatment. Aims and Objectives: This review is aimed to identify cross-contamination and spread of infection by various means and the appropriate preventive measures to be implemented. This review will also highlight the various aspects that are neglected in various dental schools/dental practice or any dental set up that potentiate cross-contamination ultimately affecting the dentist, dental team and the patients. Materials and Methods: A review of the dental literature concerning cross-contamination was performed. Material appearing in the literature before 1996 was reviewed as exhaustively as possible and materials after 1996 were reviewed electronically. In Medline, key words like cross-contamination, sterilization, asepsis, infection, infection control, prevention were used in various combinations to obtain a potential reference for review. A total of 2245 English Language titles were found, many were repeated due to recurring searches. The headings were shortlisted and reviewed for detailed examination. Results: A comprehensive review to evaluate the methods of preventing cross-contamination in dentistry involving various aspects and challenges encountered in a dental set up was constructed which was missing in the references of the review. Conclusions: Awareness and the necessary precautions play a pivotal role in preventing the occurrence of cross-contamination. It is the responsibility of the entire dental team to work in unison to prevent the menace of cross-contamination and spread of infection.

  7. Tissue Culture and Rapid Propagation of Chionanthus retusus%流苏树的组织培养和快速繁殖

    Institute of Scientific and Technical Information of China (English)

    王鑫; 孔祥生

    2014-01-01

    以流苏树种胚为试材,研究不同种类及浓度的植物生长调节剂对其无菌体系建立,带顶、侧芽茎段伸长以及生根的影响。结果表明,种胚生根最适培养基为WPM+0.1 mg∙L-1 NAA+3.0 mg∙L-1 GA3+2.0 mg∙L-16-BA,生根率为94.2%;带顶、侧芽茎段最适培养基为WPM+1.0 mg∙L-1 GA3+1.0 mg∙L-16-BA+0.5 mg∙L-1 TDZ;生根最适培养基为WPM+0.1 mg∙L-16-BA+0.5 mg∙L-1 NAA+0.5 mg∙L-1 IBA。%Using the embryo of Chionanthus retusus as the material, the effects of different concentrations and combinations of plant growth regulators on asepsis system establishment, stem segment elongation of terminal bud and lateral bud, and rooting were discussed. The results showed that the best embryo rooting medium was WPM+1.0 mg∙L-1 NAA+3.0 mg∙L-1 GA3+2.0 mg∙L-1 6-BA, the rooting rate was 94.2%, the best medium for ter-minal and laterd bud was WPM+1.0 mg∙L-1 GA3+1.0 mg∙L-1 6-BA+0.5 mg∙L-1 TDZ, and the best rooting medi-um was WPM+0.1 mg∙L-1 6-BA+0.5 mg∙L-1 NAA+0.5 mg∙L-1 IBA.

  8. Direct hair transplantation: A modified follicular unit extraction technique

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    Pradeep Sethi

    2013-01-01

    Full Text Available Background: In hair transplantation, the survival rate of harvested grafts depends upon many factors like maintenance of hydration, cold temperature, reduced mechanical handling and asepsis. All these factors are favourably improved if time out of body is reduced significantly. We have tried a modification called direct hair transplantation in the existing follicular unit extraction technique, in which the follicular unit grafts are implanted as soon as they are harvested. In this article, we have described the detailed methodology and a series of 29 patients who underwent direct hair transplantation. Aim: To evaluate the efficacy and feasibility of direct hair transplantation. Subjects and Methods: The patients willing to undergo hair transplantation by the technique of follicular unit extraction were enrolled for the surgery. After administration of local anaesthesia, the recipient sites were created. Thereafter, the processes of scoring the skin with a motorized punch, graft extraction and implantation were performed simultaneously. These patients were followed up to look for the time period of initiation of hair growth, the growth achieved at the end of 6-8 months and any adverse events. The results of patients with noticeable improvement in the photographs and reduction in baldness grade were taken as ′good′, whereas, in other patients, it was classified as ′poor′. Results: All patients were males with age ranging from 21 to 66 years (median 30 years. Twenty-six patients had androgenetic alopecia, 1 patient had traction alopecia and 2 patients had scarring alopecia. Twenty-seven patients showed ′good′ results, whereas 2 patients showed ′poor′ results. Conclusion: Direct hair transplantation is a simple and feasible modification in the follicular unit extraction technique. It is an efficacious surgical treatment modality for baldness.

  9. Acupotomology: returning to the ancients and innovation of acupuncture%针刀医学:针灸学的复古与创新

    Institute of Scientific and Technical Information of China (English)

    张义; 郭长青

    2011-01-01

    针刀治疗的实质是经皮微创软组织松解术.传统针灸学当中具备经皮微创软组织松解术,但在近现代逐渐被边缘化,因此针刀医学的发展是对针灸学的复古.与传统针灸学相比,针刀医学对经筋学说进行了现代解释,吸收了解剖学和病理学理论以及无菌和麻醉技术,并对传统针具进行了改进,因此钎刀医学又是对针灸学的创新.同时针刀医学的发展弥补了针灸学研究的不足,将在客观上促进针灸学的学科分化和学科交叉,是针灸学发展新的增长点.%Acupotomology is a technique of percutaneous minimally invasive soft tissue releasing. It can be found in traditional acupuncture, but is deemphasized in modern times. Therefore, the development of acupotomology is held as restoring of ancient ways of acupuncture. Compared with traditional acupuncture, acupotomology makes modern interpretations on the theory of muscle regions, absorbs theories of anatomy and pathology as well as techniques of asepsis and anaesthesia. It improves traditional needling tool. Therefore, it is also held as an innovation of acupuncture. The development of acupotomology makes up for the deficiency of acupuncture study. It will promote the differentiation and crossing of acupuncture discipline, and become a new trend of acupuncture.

  10. Tissue Culture of Precious Timber Species Toona ciliata Roem%珍贵用材树种红椿的组培育苗技术初探

    Institute of Scientific and Technical Information of China (English)

    陈丽文; 时群; 梁刚; 蔡林; 何贵整

    2014-01-01

    The seeds of Toona ciliata were used as explants to study the tissue culture of T. ciliata. The results showed that the MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1 media was good for the bud induction of the asepsis seedling, the optimal bud proliferation culture medium was 2/3MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1+GA3 1.0 mg·L-1, and the optimal root induction culture medium was 1/2MS+IBA 0.5 mg·L-1, The plantlet was transplanted with the matrix of the peat soil, yellow subsoil and river sand, and the survival rates up to 85%.%以红椿种子为外植体材料,对其组织培养技术进行初步研究。结果表明,适宜红椿种子无菌苗芽诱导的培养基为MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1,适宜的芽继代增殖培养基为2/3MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1+GA 31.0 mg·L-1,适宜生根培养基为1/2MS+IBA 0.5 mg·L-1,以泥炭土、黄心土、河沙按1∶1∶1混合作为基质,移栽成活率达85%以上。

  11. Antibioprophylaxis in Prevention of Endophthalmitis in Intravitreal Injection: A Systematic Review and Meta-Analysis

    Science.gov (United States)

    Benoist d’Azy, Cédric; Pereira, Bruno; Naughton, Geraldine; Chiambaretta, Frédéric; Dutheil, Frédéric

    2016-01-01

    Despite endophthalmitis being the most feared complication, antibioprophylaxis remains controversial in intravitreal injections. Therefore, we conducted a systematic review and meta-analysis on the effects of antibioprophylaxis in intravitreal injections in the prevention of endophthalmitis. The PubMed, Cochrane Library, Embase and Science Direct databases were searched for studies comparing groups with and without antibiotics in intravitreal injection, with the use of the following keywords: "antibiotic*", "endophthalmitis" and “intravitreal injection*”. To be included, studies needed to specify number of participants and number of endophthalmitis within each group (with and without antibiotics). We conducted meta-analysis on the prevalence of clinical endophthalmitis including both culture-proven and culture negative samples. Nine studies were included. A total of 88 incidences of endophthalmitis were reported from 174,159 injections (0.051% i.e., one incidence of endophthalmitis for 1979 injections). Specifically, 59 incidences of endophthalmitis were reported from 113,530 injections in the group with antibiotics (0.052% or one incidence of endophthalmitis for 1924 injections) and 29 incidences of endophthalmitis from 60,633 injections in the group without antibiotics (0.048% or one endophthalmitis for 2091 injections). Our meta-analysis did not report a significant difference in the prevalence of clinical endophthalimitis between the two groups with and without topical antibiotics: the odds ratio of clinical endophthalimitis was 0.804 (CI95% 0.384–1.682, p = 0.56) for the antibiotic group compared with the group without antibiotics. In conclusion, we performed the first large meta-analysis demonstrating that antibioprophylaxis is not required in intravitreal injections. Strict rules of asepsis remain the only evidence-based prophylaxis of endophthalmitis. The results support initiatives to reduce the global threat of resistance to antibiotics. PMID

  12. Hastane Temizlik Hizmetleri Personelinin Tibbi Atiklarin Toplanmasi, Tasinmasi ve Depolanmasi Ile Ilgili Bilgi ve Uygulamalarinin Belirlenmesi

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    Esengul Camozu

    2011-12-01

    Full Text Available Determination of Hospital Cleaning Staff’s Knowledge and Applications About Collecting, Transferring and Stocking of Medical Wastes AIM:This observational descriptive study carried out to identify cleaning staffs’ knowledge and practices about collection, transportation and storage of medical waste, at GATA Education and Research Hospital METHOD: All of 99 staffs were included in the study, Data was collected by questionnaire and observation forms. Observation form consists of 5 chapters and 36 processes created in order to observing staff’s practices and use private preventive equipments during the practices. Participants were observed twice with at least 15-day interval and then a questionnaire was administered. Written consent was obtained from staffs by informing about research before applying questionnaire. The data were analyzed by using SPSS 12 package program. RESULTS: 93 persons had been vaccinated against to HepB and 35 persons had been vaccinated against to tetanus among 99 persons were participated in study. 36 persons stated had been injured at least one time by cutting, or drilling materials. Wearing gloves were the most common private prevention (but about half of them was disposable and other private preventive materials were using less. Observed that most of the participants were carrying waste with their hands and clothes were kept in inconvenient place and washing hands, gloves and container after procedures were leaved out. Also some of participants neglected to put leaky pocket in a substantial one. CONCLUSION: Training of cleaning staffs about collect, transport and storage of medical waste relevance to fundamentals of medical asepsis also using visual stimulants and checking staff’s practices for enhancement of correct applications were suggested. [TAF Prev Med Bull 2011; 10(6.000: 631-640

  13. Effects of carbachol matching oral fluid resuscitation on intestinal mucosa blood flow and absorption rate of dogs suffered hemorrhagic shock

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    Lin LI

    2011-04-01

    Full Text Available Objective To investigate the effects of carbachol matching oral fluid resuscitation on intestinal mucosa blood flow(IMBF and intestinal absorption rate of dogs suffered hemorrhagic shock.Methods Twenty-four hours after a preliminary intubation of carotid artery,jugular vein and jejunum by asepsis,twelve Beagle dogs were subjected to a loss of 40% total blood volume to establish animal model of hemorrhagic shock.Animals were then divided into oral resuscitation group and carbachol group(6 each.Dogs in oral resuscitation group were given by gastric tube the glucose-electrolyte solution(GES,which was 3 times volume of blood loss,within 24h after bleeding;while dogs in carbachol group were given GES added carbachol(0.25μg/kg.The IMBF and intestinal absorption rate of water before hemorrhage(0h and 2,4 and 8h after hemorrhage were measured.All the animals were sacrificed at 8h after hemorrhage to record the intestinal GES volume.Results The intestinal absorption rate of water remarkably decreased after hemorrhage in both groups,while in carbachol,group it was obviously higher than that in oral resuscitation group(P < 0.05.The GES volume absorbed by intestine in carbachol group was high than that in oral resuscitation group 8h after hemorrhage(P < 0.05.The IMBF decreased significantly in the both groups after hemorrhage,and then increased gradually 2h after hemorrhage.The IMBF in carbachol group was obviously higher than that in oral resuscitation group(P < 0.05.Conclusion Carbachol in oral resuscitation with GES can improve intestinal absorption rate of water and GES,and increase IMBF in dogs with 40% blood loss.

  14. A comparative study of epidural catheter colonization and infection in Intensive Care Unit and wards in a Tertiary Care Public Hospital

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    Minal Harde

    2016-01-01

    Full Text Available Infection is a potentially serious complication of epidural analgesia and with an increase in its use in wards there is a necessity to demonstrate its safety. We aimed to compare the incidence of colonization of epidural catheters retained for short duration (for 48 h postoperative analgesia in postanesthesia care unit and wards. It was a prospective observational study done in a tertiary care teaching public hospital over a period of 2 years and included 400 patients with 200 each belonged to two groups PACU and ward. We also studied epidural tip culture pattern, skin swab culture at the entry point of the catheter, their relation to each other and whether colonization is equivalent to infection. Data were analyzed using statistical software GraphPad. Overall positive tip culture was 6% (24, of them 7% (14 were from PACU and 5% (10 were from ward (P = 0.5285. Positive skin swab culture was 38% (150, of them 20% (80 were from PACU and 18% (70 were from ward (P = 0.3526. The relation between positive tip culture and positive skin swab culture in same patients is extremely significant showing a strong linear relationship (95% confidence interval = 0.1053–0.2289. The most common microorganism isolated was Staphylococcus epidermidis. No patient had signs of local or epidural infection. There is no difference in the incidence of epidural catheter tip culture and skin swab culture of patients from the general ward and PACU. Epidural analgesia can be administered safely for 48 h in general wards without added risk of infection. The presence of positive tip culture is not a predictor of epidural space infection, and colonization is not equivalent to infection; hence, routine culture is not needed. Bacterial migration from the skin along the epidural track is the most common mode of bacterial colonization; hence, strict asepsis is necessary.

  15. Nursing of the treatment of amyotrophie lateral sclerosis applying bone mesenchymal stem cells%骨髓间充质干细胞治疗肌萎缩侧索硬化症的护理

    Institute of Scientific and Technical Information of China (English)

    王翠林; 陈惠勤; 罗利英

    2011-01-01

    目的 观察骨髓间充质干细胞(MSC)治疗肌萎缩侧索硬化症(ALS)的临床效果,探讨护理方法.方法 在无菌条件下采集患者骨髓液,密度梯度离心法分离,获取单个核细胞层,接种于培养瓶中,加入培养液,在5%二氧化碳细胞培养箱培养3d,微生物检测安全后静脉输注给患者.结果 治疗后能延迟肌萎缩侧索硬化症患者的病情进展,有效8例,无效1例.结论 MSC治疗肌萎缩侧索硬化症的临床效果有效.%Objective To observe the therapeutic effects of bone marrow-derived mesenehymal stem cells(BMSCs)in the treatment of amyotrophie lateral sclerosis(ALS).Methods To collect bone marrow in asepsis conditions.Mononuclear cells were collected by gradient centrifugation,cultured in growth medium and 5%carbon dioxide for 3 days.BMSCs were analyzed by flow cytometry with a FACScan(Becton Dickinson). All BMSCs were transplanted into patients intra venous.Results After BMSCs treatment.8 patients with ALS had obvious clinical effects,1 patients had no response to treatment.Conclusions BMSCs treatment for ALS has obvious clinical effect.

  16. 浅谈护士与医院感染%Nurses and Hospital Infection

    Institute of Scientific and Technical Information of China (English)

    卢春花

    2013-01-01

    护士是直接为患者服务,接触患者最多,频率最高,时间最长的人。护理工作是由多名护士相继交叉为患者提供所需要的各种治疗和护理。在护理过程中,护士的手、所使用的用具、无菌观念等都将成为院内感染的重要因素。因此,护士是控制医院感染的主力军,是控制医院感染的直接实施者。护士的综合素质、责任心、慎独意识与院内感染息息相关。%The nurse serves directly for the patient, contacts the patient most frequently and the time is longest. The nursing work is intersects one after another by the famous nurse each kind of treatment and nursing which provides for the patient needs. In the nursing process, the apparatus, the asepsis idea which nurse's hand and so on al becomes the important at ribute which in the hospital wil infect. Therefore, the nurse is a main army who controls the hospital infection, is direct executor who controls the hospital infection. Nurse's comprehensive quality, the sense of responsibility, are prudent when alone in consciousness and the hospital infect are closely linked.

  17. A comparative study of epidural catheter colonization and infection in Intensive Care Unit and wards in a Tertiary Care Public Hospital

    Science.gov (United States)

    Harde, Minal; Bhadade, Rakesh; Iyer, Hemlata; Jatale, Amol; Tiwatne, Sagar

    2016-01-01

    Infection is a potentially serious complication of epidural analgesia and with an increase in its use in wards there is a necessity to demonstrate its safety. We aimed to compare the incidence of colonization of epidural catheters retained for short duration (for 48 h) postoperative analgesia in postanesthesia care unit and wards. It was a prospective observational study done in a tertiary care teaching public hospital over a period of 2 years and included 400 patients with 200 each belonged to two groups PACU and ward. We also studied epidural tip culture pattern, skin swab culture at the entry point of the catheter, their relation to each other and whether colonization is equivalent to infection. Data were analyzed using statistical software GraphPad. Overall positive tip culture was 6% (24), of them 7% (14) were from PACU and 5% (10) were from ward (P = 0.5285). Positive skin swab culture was 38% (150), of them 20% (80) were from PACU and 18% (70) were from ward (P = 0.3526). The relation between positive tip culture and positive skin swab culture in same patients is extremely significant showing a strong linear relationship (95% confidence interval = 0.1053–0.2289). The most common microorganism isolated was Staphylococcus epidermidis. No patient had signs of local or epidural infection. There is no difference in the incidence of epidural catheter tip culture and skin swab culture of patients from the general ward and PACU. Epidural analgesia can be administered safely for 48 h in general wards without added risk of infection. The presence of positive tip culture is not a predictor of epidural space infection, and colonization is not equivalent to infection; hence, routine culture is not needed. Bacterial migration from the skin along the epidural track is the most common mode of bacterial colonization; hence, strict asepsis is necessary. PMID:27076712

  18. Buccal Epithelium in treating Ocular Surface Disorders

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    Srinivas KR

    2008-11-01

    Full Text Available Background - Ocular surface disorders due to limbal stem cell deficiency are an important cause of ocular morbidity and visual loss. Although autologous limbal stem cell transplants have helped in the management of unilateral disease, allografts in those with bilateral disease often fail due to immunological reasons. The use of autologous buccal epithelium cultivated on amniotic membrane has been described as a useful approach in the management of this condition. It is the purpose of this study to explore the feasibility of using a novel thermo-gelatin polymer (TGP as a substrate to culture these cells, and to characterize them using RNA extraction and RT-PCR. Methods - Oral cheek mucosal biopsies were obtained from 5 adult patients undergoing Modified Osteo-Odonto Keratoprosthesis surgery. The specimens were transported to the laboratory in transport medium. The cells were released using enzymatic digestion and seeded in both convention culture medium and TGP. The resulting cellular growth was characterized using RNA extraction and RT-PCR. Results - Cells could be cultured from 4 of the 5 specimens. In one specimen, contamination occurred and this was discarded. In the other specimens, the cheek epithelial cells could be cultured in both the conventional culture medium and TGP, with equal ease. RT-PCR revealed the presence of K3, a marker for epithelial cells, and GAPDH indicating the presence of some adipose tissue as well. Conclusions - It is possible to culture autologous cheek mucosal epithelial cells using TGP, a synthetic scaffold, without the need for other biological substrates. Since the specimens are obtained from the oral cavity, stringent asepsis is required. Further studies are required for histopathological characterization of the cultured cells and to create a model for delivery onto the ocular surface of eyes with bilateral surface disease due to limbal stem cell deficiency.

  19. Outcome of percutaneous continuous drainage of psoas abscess: A clinically guided technique

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    Bharat R Dave

    2014-01-01

    Full Text Available Background: Percutaneous aspiration of abscesses under ultrasonography (USG and computer tomography (CT scan has been well described. With recurrence rate reported as high as 66%. The open drainage and percutaneous continuous drainage (PCD has reduced the recurrence rate. The disadvantage of PCD under CT is radiation hazard and problems of asepsis. Hence a technique of clinically guided percutaneous continuous drainage of the psoas abscess without real-time imaging overcomes these problems. We describe clinically guided PCD of psoas abscess and its outcome. Materials and Methods: Twenty-nine patients with dorsolumbar spondylodiscitis without gross neural deficit with psoas abscess of size >5 cm were selected for PCD. It was done as a day care procedure under local anesthesia. Sequentially, aspiration followed by guide pin-guided trocar and catheter insertion was done without image guidance. Culture sensitivity was done and chemotherapy initiated and catheter kept till the drainage was <10 ml for 48 hours. Outcome assessment was done with relief of pain, successful abscess drainage and ODI (Oswestry Disability Index score at 2 years. Results: PCD was successful in all cases. Back and radicular pain improved in all cases. Average procedure time was 24.30 minutes, drain output was 234.40 ml, and the drainage duration was 7.90 days. One patient required surgical stabilisation due to progression of the spondylodiscitis resulting in instability inspite of successful drainage of abscess. Problems with the procedure were noticed in six patients. Multiple attempts ( n = 2, persistent discharge ( n = 1 for 2 weeks, blocked catheter ( n = 2 and catheter pull out ( n = 1 occurred with no effect on the outcome. The average ODI score improved from 62.47 to 5.51 at 2 years. Conclusions: Clinically guided PCD is an efficient, safe and easy procedure in drainage of psoas abscess.

  20. Uma abordagem fenomenológico-existencial para a questão do conhecimento em psicologia An existential-phenomenological approach to the problem of psychological knowledge

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    Marcelo Vial Roehe

    2006-08-01

    Full Text Available Partindo da analítica existencial do filósofo Martin Heidegger, o artigo propõe que o conhecimento é um correlato ontológico do modo de ser humano e que a tradição científica comete um erro ontológico quando, por meio de uma suposta assepsia metodológica, separa o conhecedor do conhecido. Sendo assim, argumenta-se que qualquer empreendimento científico está vinculado às características do ser humano, que qualquer que seja o foco de uma investigação científica, este já estará sempre submetido às possibilidades perspectivas humanas. Portanto, a objetividade que a tradição científica preconiza, de modo algum se realiza. Propõe-se também que a psicologia não necessita adotar o modelo naturalista tradicional, a fim de adquirir credibilidade científica.Based on German philosopher Martin Heidegger's existential analytic, this article proposes that knowledge is an ontological counterpart to man's mode of being, and the scientific tradition incurs in ontological error when, through the use of a so-called methodological asepsis, it separates who-knows (the "subject" of knowledge from what-is-known (the "object" of knowledge. Thus, it can be argued that any scientific enterprise is linked to the characteristics of human beings, and whatever focus a scientific investigation might have, this focus will always be limited by human perceptive capabilities and, therefore, the objectivity proclaimed by the scientific tradition is never achieved at all. The article also proposes that psychology does not need to adopt the traditional naturalistic model in order to achieve scientific credibility.

  1. Infections following the application of leeches: two case reports and review of the literature

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    Maetz Benjamin

    2012-10-01

    Full Text Available Abstract Introduction Since the 1980s, leeches have been ingeniously used in the management of venous flap congestion. The presence of anticoagulative substances in their saliva improves the blood drainage. Their digestive tract contains several bacterial species, the main ones being Aeromonas hydrophila and Aeromonas veronii biovar sobria, which contribute to the digestion of ingested blood. These bacteria can be the cause of infections. Case presentation We report two cases of septicemia related to Aeromonas veronii biovar sobria that presented after leeches had been applied to congested transverse rectus abdominis myocutaneous flaps for delayed mammary reconstructions. Patient number 1 was a 55-year-old Caucasian woman who underwent a delayed breast reconstruction procedure. On the sixth postoperative day she showed a clinical presentation of septicemia. Aeromonas veronii biovar sobria was identified in the patient’s skin and blood bacteriological samples. Her fever ceased after 4 days of antibiotic treatment. Patient number 2 was a 56-year-old Caucasian woman who underwent a delayed breast reconstruction procedure. On the seventh postoperative day we noticed that she showed a clinical presentation of septicemia. Aeromonas veronii biovar sobria was identified in the patient’s blood cultures and local bacteriological samples. An antibiogram showed resistance to amoxicillin/clavulanic acid. Her fever ceased on the eleventh postoperative day after 4 days of antibiotic treatment. Conclusion The rate of infection after application of leeches is not negligible. The concentration of Aeromonas inside the digestive tracts of leeches largely decreases when the patient is under antibiotic therapy. These germs are sensitive to third-generation cephalosporins and fluoroquinolones and resistant to amoxicillin/clavulanic acid. We recommend preventive treatment based on classical measures of asepsis and on oral antibioprophylaxy with a fluoroquinolone

  2. Seroepidemiology of Hepatitis B Virus Infection in Khuzestan Province, Southwest of Iran

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    Abuul Rahim Masjedizadeh

    2009-02-01

    Full Text Available Background: Hepatitis B is a disease of public health importance in Iran. We conducted this study to determine the epidemiology, clinical presentations of hepatitis B infection in Khuzestan province, Southwest of Iran.Methods: A cross-sectional study was conducted on hepatitis B virus (HBV positive individuals referred to the Ahwaz Jondishapour University Hospitals (AJSUH and Hepatitis Clinic from February 2002 to May 2007. Based on a specially-designed protocol, standard commercially-available tests and physical examinations were performed. All subjects were evaluated using a face-to-face questionnaire about demographic aspects. The analysis included data on past medical history, physical examination and periodic evaluation clinically and serologically.Results: 1264 patients infected with hepatitis B virus participated in the study. The patients consisted of 874 male and 390 female patients with the age range of 8-72 years. The most frequent age group was 20-40 years (56.4%. Anti-delta antibodies were observed in 4.7% of the carriers (59⁄1264, anti hepatitis C virus (anti-HCV antibodies in 0.9% (12⁄1095 and anti human immunodeficiency virus (anti-HIV antibodies in 0.1% (1⁄1095 of active carriers, respectively. Of 1264 patients infected with hepatitis B virus, 71 (5.6% were also hepatitis B e antigen (HBeAg positive, no difference in female to male ratio was observed between HBeAg-positive and HBeAg-negative patients with chronic hepatitis B.Conclusions: The family history of hepatitis, dental procedures and a history of transfusion are important risk factors for HBV infection in our area. More careful screening and preventive measures, strict attention to asepsis, evaluation of risk factors, and improvements in certain lifestyle patterns and customs in this area may be essential to prevent transmission of the infection.

  3. Application of continuous quality improvement in central sterilization supply department%持续质量改进在消毒供应中心的应用

    Institute of Scientific and Technical Information of China (English)

    冯鸿雁

    2011-01-01

    OBJECTIVE To apply continuous quality management mode combining the characteristics of the center of sterilization supply, to continuously improve the quality.METHODS Process management mode was adopted,efficient continuous quality control work team was established, quality evaluation system was perfected.RESULTS Various disinfection segregation indexes complied with the ministry of health "disinfect technical specification of line marking requirements".The qualified rate of monitoring asepsis items reached to 100.0%.Monitoring of the center of sterilization supply infection control work was more standardized, standard and scientific.CONCLUSION Continuous quality improvement in the center of sterilization supply has been achieved remarkable effects, and also promoted the level of nursing management.%目的 应用持续质量管理模式,结合消毒供应中心的特点,对其进行持续质量改进.方法 采用过程管理模式,建立高效持续质量控制工作团队,健全质量评价体系.结果 各项消毒隔离指标符合卫生部的行标要求,无菌物品监测合格率达100.O%,消毒供应中心的感染控制工作更规范化、标准化、科学化.结论 持续质量改进在消毒供应中心取得显著成效,提升了护理管理水平.

  4. Antibioprophylaxis in Prevention of Endophthalmitis in Intravitreal Injection: A Systematic Review and Meta-Analysis.

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    Cédric Benoist d'Azy

    Full Text Available Despite endophthalmitis being the most feared complication, antibioprophylaxis remains controversial in intravitreal injections. Therefore, we conducted a systematic review and meta-analysis on the effects of antibioprophylaxis in intravitreal injections in the prevention of endophthalmitis. The PubMed, Cochrane Library, Embase and Science Direct databases were searched for studies comparing groups with and without antibiotics in intravitreal injection, with the use of the following keywords: "antibiotic*", "endophthalmitis" and "intravitreal injection*". To be included, studies needed to specify number of participants and number of endophthalmitis within each group (with and without antibiotics. We conducted meta-analysis on the prevalence of clinical endophthalmitis including both culture-proven and culture negative samples. Nine studies were included. A total of 88 incidences of endophthalmitis were reported from 174,159 injections (0.051% i.e., one incidence of endophthalmitis for 1979 injections. Specifically, 59 incidences of endophthalmitis were reported from 113,530 injections in the group with antibiotics (0.052% or one incidence of endophthalmitis for 1924 injections and 29 incidences of endophthalmitis from 60,633 injections in the group without antibiotics (0.048% or one endophthalmitis for 2091 injections. Our meta-analysis did not report a significant difference in the prevalence of clinical endophthalimitis between the two groups with and without topical antibiotics: the odds ratio of clinical endophthalimitis was 0.804 (CI95% 0.384-1.682, p = 0.56 for the antibiotic group compared with the group without antibiotics. In conclusion, we performed the first large meta-analysis demonstrating that antibioprophylaxis is not required in intravitreal injections. Strict rules of asepsis remain the only evidence-based prophylaxis of endophthalmitis. The results support initiatives to reduce the global threat of resistance to antibiotics.

  5. In vitro antifungal action of different substances over microwaved-cured acrylic resins

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    Henrique Montagner

    2009-10-01

    Full Text Available OBJECTIVE: The presence of Candida albicans on the surfaces of denture-base acrylic resins is strongly related to the development of oral stomatitis. This study evaluated the antifungal action of different agents over microwave-cured acrylic resin without polishing specimens previously contaminated with Candida albicans. MATERIAL AND METHODS: Sixty specimens were immersed in BHI broth previously inoculated with the yeast and stored for 3 h at 37ºC. They were divided into 5 experimental groups (n=10: G1: 2% chlorhexidine solution (10 min; G2: 0.5% sodium hypochlorite (10 min; G3: modified sodium hypochlorite (10 min; G4: effervescent agent (5 min; G5: hydrogen peroxide 10v (30 min. The specimens of the control group 1 (C1 were not disinfected. Ten additional specimens of the control group 2 (C2 were not infected with the yeast, aiming to check the asepsis during the experiment. The disinfection agents were neutralized and the acrylic resin specimens were immersed in BHI Broth for 24 h. Culture media turbidity was evaluated spectrophotometrically according to the transmittance degree, i.e. the higher the transmittance the stronger the antimicrobial action. Statistical analysis was performed (Kruskal-Wallis Test, p<0.05. RESULTS: The results, represented by the medians, were: G1 = 40; G2 = 100; G3 = 100; G4 = 90; G5 = 100; C1 = 40; C2 = 100. CONCLUSIONS: This in vitro study suggested that sodium hypochlorite-based substances and hydrogen peroxide are more efficient disinfectants against C. albicans than 2% chlorhexidine solution and the effervescent agent.

  6. Cosmetic outcome and surgical site infection rates of antibacterial absorbable (Polyglactin 910) suture compared to Chinese silk suture in breast cancer surgery: a randomized pilot research

    Institute of Scientific and Technical Information of China (English)

    ZHANG Zhong-tao; Jessica Shen; Martin Weisberg; ZHANG Hong-wei; FANG Xue-dong; WANG Li-ming; LI Xiao-xi; LI Ya-fen; SUN Xiao-wei; Judith Carver; Dorella Simpkins

    2011-01-01

    Background The primary objective of this multicenter post-market study was to compare the cosmetic outcome of triclosan-coated VICRYL* Plus sutures with Chinese silk sutures for skin closure of modified radical mastectomy. A secondary objective was to assess the incidence of surgical site infection (SSI).Methods Patients undergoing modified radical mastectomy were randomly assigned to coated VICRYL* Plus antibacterial (Polyglactin 910) suture or Chinese silk suture. Cosmetic outcomes were evaluated postoperatively at days 12 (±2) and 30 (±5), and the evidence of SSI was assessed at days 3, 5, 7, 12 (±2), 30 (±5), and 90 (±7). Cosmetic outcomes were independently assessed via visual analogue scale (VAS) score evaluations of blinded incision photographs (primary endpoint) and surgeon-assessed modified Hollander Scale (mHCS) scores (secondary endpoint).SSI assessments used both CDC criteria and ASEPSIS scores.Results Six Chinese hospitals randomized 101 women undergoing modified radical mastectomy to closure with coated VICRYL* Plus suture (n=51) or Chinese silk suture (n=50). Mean VAS cosmetic outcome scores for antibacterial suture (67.2) were better than for Chinese silk (45.4) at day 30 (P<0.0001)). Mean mHCS cosmetic outcome total scores, were also higher for antibacterial suture (5.7) than for Chinese silk (5.0) at day 30 (P=0.002).Conclusions Patients using coated VICRYL* Plus suture had significantly better cosmetic outcomes than those with Chinese silk sutures. Patients using coated VICRYL* Plus suture had a lower SSI incidence compared to the Chinese silk sutures, although the difference did not reach statistical significance. (ClinicalTrials.gov NCT 00768222)

  7. 高中效过滤、高强度紫外线和动态离子杀菌组合空气卫生工程新技术在手术室的应用%The Combination of the New Air Hygiene Engineering Technology of High & Medium Efficiency Filter, High Intensity UVC and Dynamic Ionic Sterilization in Operation Room

    Institute of Scientific and Technical Information of China (English)

    徐火炬

    2012-01-01

    采用高中效空气过滤、高强度风管紫外线辐照和室内空气动态离子杀菌组合空气卫生工程新技术的手术室可以达到优于高效过滤洁净技术的空气无菌效果,洁净度指标也达到一定的净化级别,节能50%以上,并具有洁净技术不具备的手术中房间空气和表面直接瞬时杀菌功能,有利于减少采用传统洁净技术的手术室难以避免的手术组人员产生的手术中的空气局部污染对切口感染的影响和明显降低造价和运行费。%The operation room which adopt the combination of high & medium efficiency air filtering, high intensity duct UVC irradiation and indoor dynamic ionic sterilization the new air hygiene engineering technology can reach the better asepsis effect than HEPA filter, and the clean class has also reached certain level of cleanliness, the energy saving more than 50%. It provides the air and surface directly instantaneous sterilize during operation that clean technology does not have the function, and help to reduce the effect on wound infecting from air local pollution cause by the surgical teams during the operation that traditional cleanroom technology can not settle this problem and has significant reduce fabrication cost and running cost.

  8. 软性角膜接触镜在角膜外伤中的应用%Soft corneal contact lens in the treatment of corneal trauma

    Institute of Scientific and Technical Information of China (English)

    丰香兰; 梁惠英; 张宁; 张玉环; 田玉民

    2001-01-01

    Objective:To evaluate the therapeutic effect of the soft corneal contact lens in the treatment of corneal trauma.Method:63 eyes of 59 cases with corneal,including 9 eyes not sutured with penetrating injury of corneal,39 eyes postsutured,15 eyes with chemical and thermic burns,were treated with soft corneal contact lens.After dressing for two or three days,they wore the soft corneal contact lens on the condition of asepsis,frequently local medication and application of general medication.Results:After being worn the soft corneal contact lens,the symptom of stimulation was eliminated.Corneal epithelial renewed rapidly,wound recovery stage was shortened.There was no complications. The visual acuity was improved to some degree.Conclusion:During the treatment of corneal trauma,the soft corneal contact lens and reasonable medication make complementation.It also can promote wound healing,shorten the course of the treatment and reduce the complications.%目的:评价软性角膜接触镜治疗角膜外伤的疗效。方法:配戴软性角膜接触镜治疗角膜外伤59例、63眼,包括角膜穿通伤未缝合9眼,缝合术后39眼,化学及热烫伤11例、15眼,伤后包扎2~3d,无菌条件下戴镜,局部用药,配合全身用药。结果:戴镜后刺激症状消除,角膜上皮修复快,伤口愈合期缩短,无并发症发生,视力均有不同程度的提高。结论:在角膜外伤治疗过程中,角膜接触镜与全身及局部合理用药,有助于促进伤口愈合,缩短疗程,减少并发症。

  9. Fototerapia (LEDs 660/890nm no tratamento de úlceras de perna em pacientes diabéticos: estudo de caso Phototherapy (LEDs 660/890nm in the treatment of leg ulcers in diabetic patients: case study

    Directory of Open Access Journals (Sweden)

    Débora Garbin Minatel

    2009-07-01

    Full Text Available Avaliou-se a fototerapia na cicatrização de úlceras de perna (UP mistas em dois pacientes diabéticos (tipo 2, hipertensos. O aparelho apresentava sonda 1 (S1 (1 LED de 660nm, 5mW aplicado em 3 UP e sonda 2 (S2 (32 LEDs de 890nm e 4 LEDs de 660nm, 500mW em 6 UP. Após antissepsia,úlceras foram tratadas com sondas a 3J/cm2, 30seg, 2x/semana seguido pelo curativo diário com sulfadiazina de prata a 1% por 12 semanas. Pela análise com software Image J®, as UP com S2 tiveram índices de cicatrização médios de 0,6; 0,7 e 0,9 enquanto S1 foi de 0,2; 0,4 e 0,6 no 30º, 60º e 90º dias, respectivamente. A fototerapia acelerou a cicatrização das úlceras de perna em pacientes diabéticos.This study evaluated the use of phototherapy in the healing of mixed leg ulcers in two diabetic patients (type 2 with arterial hypertension. The device had probe 1 (one 660nm LED, 5mW applied in 3 ulcers and probe 2 (32 890nm LEDs associated with 4 660nm LEDs, 500mW in 6 ulcers. After asepsis, ulcers were treated with probes to 3 J/cm2, 30sec per point, twice a week, followed by topical daily dressing with 1% silver sulphadiazine during 12 weeks. The following analyses of ulcers with software Image J showed that probe 2 presented mean healing rates of 0.6; 0.7 and 0.9, whereas probe 1 had 0.2;0.4 and 0.6 at 30, 60 and 90 days, espectively. Phototherapy accelerated wound healing of leg ulcers in diabetic patients.

  10. Qual seria a fonte de fungos miceliais encontrados em leite humano ordenhado? What is the source of mycelial fungi in expressed human milk?

    Directory of Open Access Journals (Sweden)

    Franz Reis Novak

    2002-06-01

    Full Text Available Caracterizou-se os gêneros de fungos miceliais encontrados em amostras de leite humano ordenhado, recebidas a partir de coleta domiciliar, pelo Banco de Leite Humano do Instituto Fernandes Figueira. Foram analisadas 821 amostras de leite humano ordenhado, obtidas ao acaso, a partir de frascos coletados nos domicílios pelas próprias doadoras, e realizadas pesquisas de bolores e leveduras e microrganismos mesófilos. As análises revelaram a ocorrência de bolores e leveduras em 43 (5,2% das amostras, com contagens atingindo a ordem de 10³UFC/ml, tendo sido isoladas 48 cepas de fungos miceliais, que foram identificadas por técnicas padrão de laboratório, como as seguintes: Aspergillus Grupo Niger (6,3%, Aspergillus sp. (4,2%, Paecilomyces sp. (12,6%, Penicillium sp. (60,4%, Rhizopus sp. (2,0% e Syncephalastrum sp. (14,5%. Discute-se a importância do controle da assepsia das mãos das doadoras, antes da coleta do leite humano.The authors characterized the genera of mycelial fungi found in samples of expressed human milk received through home collection by the Human Milk Bank of the Instituto Fernandes Figueira in Rio de Janeiro. A total of 821 samples of expressed human milk were taken randomly from bottles collected at home by the milk donors themselves and were investigated for molds, yeasts, and mesophilic microorganisms. The analyses showed the occurrence of molds and yeasts in 43 (5.2% of the samples, with counts reaching 103CFU/ml. Some 48 strains of mycelial fungi were identified by standard laboratory techniques, including: Aspergillus Niger group (6.3%, Aspergillus sp. (4.2%, Paecilomyces sp. (12.6%, Penicillium sp. (60.4%, Rhizopus sp. (2.0%, and Syncephalastrum sp. (14.5%. The authors discuss the importance of donor hands' asepsis prior to collecting human milk.

  11. 琼胶寡糖激发坛紫菜对附生细菌的减少作用%Oligoagars Induce Defense Response of Porphyra haitanensis to Epiphytic Bacteria

    Institute of Scientific and Technical Information of China (English)

    朱方超; 李崇丰; 王秀娟; 朱竹君; 陈海敏; 严小军

    2012-01-01

    The rot disease of Porphyra haitanens& cultured under 18℃ and 35℃ were obviously lightened by pretreatment with oligoagars. The defense response elicited by oligoagars was strong enough to kill epiphytic bacteria associated with the algal. Up to 94.7% of the resident bacterial flora of healthy plants was eliminated within 60 min after treatment by 100μg.mL-1 oligoagars. Addition of catalase prior to oligoagars partially inhibited this elimination. Mixture of three kinds of antibiotics was used to treat P haitanensis to get the asepsis culture systems. Approximately 60.8% of reinfected pathogenic bacteria on axenic algal were eliminated within 3 h after elicitation by oligoagars. Antimicrobial test revealed that oligoagars showed no direct toxicity to associated bacteria or pathogen, but a rapid release of hydrogen peroxide was observed after adding of oligoagars in medium. From these results, we infer that oligoagars can be recognized as defense elicitors by P. haitanensis to protect algal efficiently from infections by eliminating the associated pathogenic bacteria.%以琼胶寡糖处理坛紫菜Porphyra haitanensis,激发其防御反应,坛紫菜在18℃生长和35℃热激时的病烂现象得到明显改善。寡糖激发的防御信号能够杀死附生在藻上的细菌,健康藻上约94.7%的附生菌群在加入100μg.mL-1琼胶寡糖1 h内被杀死,但过氧化氢酶可部分抑制这种作用。利用3种抗生素组合获得无菌坛紫菜体系,回染致病细菌后,约60.8%的回染细菌在寡糖激发后的3 h内被消灭。这种附生细菌的减少现象不是源于琼胶寡糖的直接抑菌作用,可能与琼胶寡糖引起坛紫菜H2O2的瞬时暴发有关。结果表明,琼胶寡糖能够作为坛紫菜的抗性激发子,通过诱导坛紫菜自身的防御反应来减少附生细菌的数量,从而保持藻体的健康生长。

  12. Ionic implantation by plasma in titanium and stainless steels used in prosthesis and medical instruments; Implantacion ionica por plasma en titanio y aceros inoxidables usados en protesis e instrumental medico

    Energy Technology Data Exchange (ETDEWEB)

    Munoz C, A. E. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)

    2008-07-01

    A study of a process known as plasma immersion ion implantation (PIII) of nitrogen at low voltages (< 4 kV) into three kind of samples: 1) austenitic stainless AISI 316-L steel plates, 2) ferritic stainless AISI 434 steel-based dentistry drills and 3) commercially pure titanium (CPTi) disks. On the case of CPTi the study was conducted in nitrogen- oxygen calibrated mixtures: 90% N-10% O, 80% N-20% O, 70% N-30% O and in 99.5% pure oxygen and 99.9% pure nitrogen. The PIII process was carried out by using a direct current plasma source controlled both in voltage and current, a negative voltage pulse modulator, a stainless AISI 304 steel vacuum chamber and a rod of the same material, horizontally located in the upper region of the chamber, which plays the role of anode in the plasma discharge. The purpose of the nitriding is forming a relatively thick layer on the surface of the steel specimens in order to enhance their both microhardness and general corrosion performances, desirable in medical applications. This layer contains interstitial nitrogen atoms ({approx}24% at.) which gives place to a deformed lattice (expanded phase) of the steel. Vickers microhardness and potentiodynamic tests (the latter in agreement to the norm ASTM G-61-89) confirm an increase of microhardness up to three times and a decrease of general corrosion rate in one order of magnitude. The nitriding of de dentistry drills is aimed at inhibiting the pitting corrosion produced by the asepsis process which results in pit nucleations, their propagation and consequent fractures when being under cyclic stress (fatigue). Scanning electron microscope micrographs reveal the risks involved in surpassing the critical treatment simple temperature of 450 C as the PIII process itself induces pitting. On its part, cyclic (ASTM G-61) potentiodynamic tests indicate an excellent pitting corrosion resistance of the samples treated under 450 C. In turn, the treatment of CPTi was meant to develop oxidized and

  13. [Sciatica. From stretch rack to microdiscectomy].

    Science.gov (United States)

    Gruber, P; Böni, T

    2015-12-01

    In ancient times as well as in the Middle Ages treatment options for discogenic nerve compression syndrome were limited and usually not very specific because of low anatomical and pathophysiological knowledge. The stretch rack (scamnum Hippocratis) was particularly prominent but was widely used as a therapeutic device for very different spinal disorders. Since the beginning of the nineteenth century anatomical knowledge increased and the advances in the fields of asepsis, anesthesia and surgery resulted in an increase in surgical interventions on the spine. In 1908 the first successful lumbar discectomy was initiated and performed by the German neurologist Heinrich O. Oppenheim (1858-1919) and the surgeon Fedor Krause (1857-1937); however, neither recognized the true pathological condition of discogenic nerve compression syndrome. With the landmark report in the New England Journal of Medicine in 1934, the two American surgeons William Jason Mixter (1880-1958) and Joseph Seaton Barr (1901-1963) finally clarified the pathomechanism of lumbar disc herniation and furthermore, propagated discectomy as the standard therapy. Since then interventions on intervertebral discs rapidly increased and the treatment options for lumbar disc surgery quickly evolved. The surgical procedures changed over time and were continuously being refined. In the late 1960s the surgical microscope was introduced for spinal surgery by the work of the famous neurosurgeon Mahmut Gazi Yasargil and his colleague Wolfhard Caspar and so-called microdiscectomy was introduced. Besides open discectomy other interventional techniques were developed to overcome the side effects of surgical procedures. In 1964 the American orthopedic surgeon Lyman Smith (1912-1991) introduced chemonucleolysis, a minimally invasive technique consisting only of a cannula and the proteolytic enzyme chymopapain, which is injected into the disc compartment to dissolve the displaced disc material. In 1975 the Japanese orthopedic

  14. Ionic implantation by plasma in titanium and stainless steels used in prosthesis and medical instruments

    International Nuclear Information System (INIS)

    A study of a process known as plasma immersion ion implantation (PIII) of nitrogen at low voltages (< 4 kV) into three kind of samples: 1) austenitic stainless AISI 316-L steel plates, 2) ferritic stainless AISI 434 steel-based dentistry drills and 3) commercially pure titanium (CPTi) disks. On the case of CPTi the study was conducted in nitrogen- oxygen calibrated mixtures: 90% N-10% O, 80% N-20% O, 70% N-30% O and in 99.5% pure oxygen and 99.9% pure nitrogen. The PIII process was carried out by using a direct current plasma source controlled both in voltage and current, a negative voltage pulse modulator, a stainless AISI 304 steel vacuum chamber and a rod of the same material, horizontally located in the upper region of the chamber, which plays the role of anode in the plasma discharge. The purpose of the nitriding is forming a relatively thick layer on the surface of the steel specimens in order to enhance their both microhardness and general corrosion performances, desirable in medical applications. This layer contains interstitial nitrogen atoms (∼24% at.) which gives place to a deformed lattice (expanded phase) of the steel. Vickers microhardness and potentiodynamic tests (the latter in agreement to the norm ASTM G-61-89) confirm an increase of microhardness up to three times and a decrease of general corrosion rate in one order of magnitude. The nitriding of de dentistry drills is aimed at inhibiting the pitting corrosion produced by the asepsis process which results in pit nucleations, their propagation and consequent fractures when being under cyclic stress (fatigue). Scanning electron microscope micrographs reveal the risks involved in surpassing the critical treatment simple temperature of 450 C as the PIII process itself induces pitting. On its part, cyclic (ASTM G-61) potentiodynamic tests indicate an excellent pitting corrosion resistance of the samples treated under 450 C. In turn, the treatment of CPTi was meant to develop oxidized and

  15. Microbiological analysis of critical points in the chicken industry

    Directory of Open Access Journals (Sweden)

    Rogério Luis Cansian

    2005-05-01

    Full Text Available This work is focused on identifying microbial contamination in the scalding asepsis and cooling processes as well as in fresh sausages obtained. Salmonella was identified in two scald water samples but was absent in the water from chiller and in the final product, which might be explained in terms of chlorine addition and temperature reduction. The analysis revealed that MPN of Escherichia coli was in the range of O objetivo deste trabalho foi identificar a contaminação microbiana no processo de escaldagem, assepsia e resfriamento do frango (chiller, e em linguiças de frango produzidas a partir destes. As amostras foram coletadas em um frigorífico de aves, em sete datas e analisadas em triplicata. A presença de Salmonella foi detectada em duas amostras da água de escaldagem não estando mais presente na água do chiller e nem no produto final. Isto se deve à redução de temperatura da água e adição de cloro. O NMP de coliformes fecais variou entre < 1 a 11/ml na água de escaldagem e < 1 a 64/ml na água do chiller, que embora em padrões aceitáveis, mostram tendência de acréscimo no chiller, devido principalmente ao processo de evisceração. As contagens de Aeromonas variaram de 5 a 3,5x10¹UFC/ml na água de escaldagem e 9 a 3,7x10²UFC/ml na água do chiller. Este acréscimo se deve, provavelmente, por Aeromonas ser psicrófila e também devido a retirada das víceras. As análises de linguiça de frango mostraram acréscimo nas contagens de Aeromonas, apresentando até 2,5x10³UFC/g. Esta tendência de aumento de crescimento no produto final, aliado a capacidade de causar infecções de Aeromonas demonstram a necessidade de incluir a análise destas nas avaliações microbiológicas de alimentos.

  16. A Histologic Evaluation on Tissue Reaction to Three Implanted Materials (MTA, Root MTA and Portland Cement Type I in the Mandible of Cats

    Directory of Open Access Journals (Sweden)

    F. Sasani

    2004-09-01

    Full Text Available Statement of Problem: Nowadays Mineral Trioxide aggregate (MTA is widely used for root end fillings, pulp capping, perforation repair and other endodontic treatments.Investigations have shown similar physical and chemical properties for Portland cement and Root MTA with those described for MTA.Purpose: The aim of this in vitro study was to evaluate the tissue reaction to implanted MTA, Portland cement and Root MTA in the mandible of cats.Materials and Methods: Under asepsis condition and general anesthesia, a mucoperiosteal flap, following the application of local anesthesia, was elevated to expose mandibular symphysis. Two small holes in both sides of mandible were drilled. MTA, Portland cement and Root MTA were mixed according to the manufacturers, recommendation and placed in bony cavities. In positive control group, the test material was Zinc oxide powder plus tricresoformalin. In negative control group, the bony cavities were left untreated. After 3,6 and 12 weeks, the animals were sacrificed and the mandibular sections were prepared for histologic examination under light microscope. The presence and thickness of inflammation, presence of fibrosis capsule, the severity of fibrosis and bone formation were investigated. The data were submitted to Exact Fisher test, chi square test and Kruskal-Wallis test for statistical analysis.Results: No statistically significant differences were found in the degree of inflammation,presence of fibrotic capsule, severity of fibrosis and inflammation thickness between Root MTA, Portland cement and MTA (P>0.05. There was no statistical difference in boneformation between MTA and Portland cement (P>0.05. However, bone formation was not found in any of the Root MTA specimens and the observed tissue was exclusively of fibrosis type.Conclusion: The physical and histological results observed with MTA are similar to those of Root MTA and Portland cement. Additionally, all of these three materials are biocompatible

  17. Institutos de pesquisa em saúde Health research institutes

    Directory of Open Access Journals (Sweden)

    Erney Plessmann Camargo

    2004-06-01

    Full Text Available Ao fim do século 19, em seguida aos avanços na assepsia cirúrgica e no esclarecimento do papel de microorganismos como causadores de moléstias infecciosas, foram criados em escala mundial "institutos de pesquisa com os objetivos de conduzir pesquisa sobre as causas, prevenção e tratamento de homens e animais e de desenvolver produtos preventivos e curativos como vacinas e antitoxinas". Para atingir esses objetivos os institutos recrutaram os melhores cientistas disponíveis em todos os lugares. Este foi um período fulgurante para a pesquisa sublinhado por inúmeras descobertas sobre as doenças infecciosas. Passada essa fase, os institutos entraram em depressão e até mesmo insolvência. Muitos se recuperaram, alguns não. Muitos ainda sofrem de uma "crise de identidade". Neste ensaio analisamos o destino dos institutos de pesquisa em saúde no Brasil, as causas de seu sucesso e eventuais fracassos e as medidas possíveis de ajudá-los a contornar suas presentes dificuldades.By the end of the 19th century, after the advances in surgical asepsis and the understanding of the role of microorganisms in the etiology of many infectious diseases, research institutes in the public health area were created worldwide with the objectives of undertaking "scientific research into the causes, prevention and treatment of disease in man and animals and to prepare and supply protective and curative materials such as vaccines and antitoxins". To pursue these objectives the institutes hired the best scientists from everywhere. This was an exhilarating period for research in which discoveries proliferated. After that, institutes got into depression and even insolvency. Most recovered, many not. Many are still suffering from an "identity crisis". In this paper we analyze the fate of the health research institutes in Brazil, the causes of their success and eventual failures and the possible ways to get out of their current difficulties.

  18. Tissue Culture of Endangered Plant Betula microphylla var.paludosa and Its Pilotscale Experiment in Shanghai Area%濒危植物沼泽小叶桦组织培养技术及其在上海地区的中试

    Institute of Scientific and Technical Information of China (English)

    张群

    2012-01-01

    为快速获得大量沼泽小叶桦苗,通过对茎尖进行丛生芽诱导、生根、移栽,成功获得了沼泽小叶桦组培苗.研究了沼泽小叶桦外植体不同灭菌方法、培养基等因素对沼泽小叶桦组培的影响.结果表明,10%次氯酸钠作为外植体消毒剂优于0.1%升汞,适宜的丛生芽诱导增殖培养基为MS+0.6 mg/L 6-BA+3%蔗糖+O.7%琼脂,最佳生根培养基为MS+3%蔗糖+0.7%琼脂.无菌苗移栽在蛭石∶珍珠岩∶草炭=3∶3∶4的介质中,成活率达100%,生长良好.%A large number of seedlings of Betula microphylla var. Paludosa was achieved by inducing adventitious buds, rooting and transferring. The effects of different disinfection methods, culture medium and proportions of transferring media on explants Betula microphylla var. Paludosa were investigated. The result showed that the disinfection effect of 10% sodium hypochlorite was better than 0.1% mercuric chloride. The appropriate culture medium for adventitious bud inducing proliferation was MS + 0. 6 mg/L 6-BA + 30 g/L sucrose + 7 g/L agar. The optimized rooting medium was MS + 30 g/L sucrose + 7 g/ L agar. The most effective medium for livability of asepsis sprout transferring of Betula microphylla var. Paludosa was vermiculite : per lite : peat = 3 : 3 : 4, and its surviving rate reached 100%. Tissue culture seedlings grew well in Shanghai area.

  19. In vitro callus induction and plantlet regeneration of Achyranthes aspera L., a high value medicinal plant

    Institute of Scientific and Technical Information of China (English)

    Monokesh Kumer Sen; Shamima Nasrin; Shahedur Rahman; Abu Hena Mostofa Jamal

    2014-01-01

    Objective:To study callus induction from different explants (internode, leaf, root) and in vitro plantlets propagation from medicinally important plant Achyranthes aspera L. Methods:Sterilized explants were prepared by using 0.1%HgCl2 and 0.5%Bavistin and callus was obtained when cultured onto Murashige Skoog’s (MS) medium by using different concentrations and combination of 2,4-D, NAA, BAP, IAA, IBA with 3%sucrose and 0.8%agar. Induced callus was immediately transferred to MS medium containing at different concentrations of phytohormones for shootlets and rootlets induction respectively. Results:Sterilization treatment of 0.1%HgCl2 for 2-3 min and Bavistin 0.5%for 10-12 min showed the highest percentage of asepsis and survival rate. Maximum induction of callus was obtained from a combination of 2.0 mg/L 2,4-D and 0.5 mg/L NAA from leaf. Highest shootlets number (4.83±0.17) and length (3.8±0.16) cm were observed on full strength MS medium when fortified with BAP 4.0 mg/L and KIN 0.5 mg/L. Concerted efforts of BAP 2.0 mg/L and NAA 0.5 mg/L on full strength MS medium showed highest leaf number (6.77±0.94). In vitro raised shoots were allowed to root on different strengths of MS medium fortified with IAA and IBA at different concentrations. Experimentally, 3.0 mg/L IBA was enabled to induce maximum rootlets number (10.0±9.82) on full strength MS medium. Afterwards, regenerated shoots with well developed roots were successfully subjected to hardening process and were acclimatized. The survived plantlets showed 66.67%survival frequency without any morphological abnormality. Conclusions: The results demonstrated that different explants were good source of callus induction, morphology analysis as well as indirect plantlets regeneration.

  20. The Boundaries of Imposture. Reflections on Ethnographic Work Among Religious Minorities

    Directory of Open Access Journals (Sweden)

    Cantón Delgado, Manuela

    2008-06-01

    Full Text Available The author discusses the admission of subjectivity as a task in the service of the ideal of scientific asepsis. Those who argue that the interference caused by the impact of the idiosyncratic factor on the objectivity ideal can be eliminated by techniques of confession and self —control approve of, beyond the unlikely efficiency of such measures, the acknowledgement of objectivity as the ultimate goal of anthropological practice. In the ethnography of religions, the two— way suspicion between the researchers and the researched permeates into all aspects of the inquiry; fills with uncertainties the relationships with our informants and with our colleagues; and is present in our readings, in the texts we write and in the keys with which we interpret; in sum, conditions the production of knowledge from beginning to end, and takes us to ask ourselves for the meaning of that ideal at times when the very boundaries of imposture are often at stake.

    En este texto se discute el reconocimiento de la subjetividad como tarea puesta al servicio del ideal de asepsia científica. Quienes postulan que la interferencia causada por el impacto del factor idiosincrásico sobre el ideal de objetividad puede ser conjurada mediante técnicas de confesión y autocontrol aprueban, más allá de la improbable eficacia de tales medidas, el reconocimiento de la objetividad como meta última de la actividad antropológica. En la etnografía con religiones, la sospecha de doble dirección, entre investigadores e investigados, se cuela por todas las rendijas del trabajo; impregna de incertidumbres las relaciones con nuestros informantes, con nuestros propios colegas; está presente en las lecturas que hacemos, los textos que escribimos y las claves con las que interpretamos. Condiciona, en suma, la producción de conocimiento de principio a fin, y nos lleva a preguntarnos por el sentido de aquel ideal, cuando lo que a menudo está en juego son los límites mismos

  1. 隐源性脑脓肿16例临床分析%Cryptogenic Brain Abscess:a Report of 16 Cases

    Institute of Scientific and Technical Information of China (English)

    王卫民; 肖国民; 裘五四; 姜启周; 程军; 吴建跃

    2009-01-01

    OBJECTIVE To study the clinical diagnosis and treatment of cryptogenic brain abscess. METHODS Sixteen cases with brain abscess of unknown sources of infection during the past 11 years were analyzed. RESULTS Of 16 patients, 13 patients got bacterial culture, and showed staphylococcus asepsis in 4 cases, Streptococcus pyo-genes in 3 cases,Escherichia coli in 2 cases,Klebsiella pneumoniae in 1 case and aseptic growth in 3 cases. Fifteen cases were cured, 1 case died of multiple organs function failure. Of cured cases , the exception of 1 case of elderly patients have had muscle strength slightly worse in the side of the distal limb, the remaining cases were good. CONCLUSIONS The clinical manifestations of cryptogenic brain abscess are not typical. The abscess could be more common in elderly than younger. To timely diagnose and treat, the prognosis is good. Abscess puncture and drainage are the first choice. Small brain abscess could be cured by conservative treatment of antibiotics.%目的 探讨隐源性脑脓肿的临床诊断及治疗问题.方法 分析1997年1月-2008年3月医院收治的16例病原学来源不明确、病程表现不典型的脑脓肿患者.结果 13例手术患者脓液培养出金黄色葡萄球菌4株、酿脓链球菌3株、大肠埃希菌2株、肺炎克雷伯菌1株;治愈15例,自动出院1例,治愈病例除1例老年患者轻偏瘫外,其余恢复均良好.结论 隐源性脑脓肿临床表现呈非特异性,以革兰阳性菌感染为主,老年人比例高;及时明确诊断及治疗,预后良好,手术以穿刺引流为首选,小型脑脓肿抗菌药物保守治疗也可治愈.

  2. 超高压处理对菠萝汁品质的影响%Effect of Ultra-high Pressure Treatment on Quality of Pineapple Juice

    Institute of Scientific and Technical Information of China (English)

    张微; 李汴生

    2009-01-01

    分析了超高压(UHP)处理对菠萝汁杀菌效果、理化指标、感官品质的影响.结果表明:随着压力值(300-500 MPa)的上升菌落总数逐渐减少,400 MPa压力下达商业无菌;pH值、可溶性固形物含量与对照样差异不显著(P>0.05);浊度随着压力上升逐渐下降,当压力超过450 MPa,浊度下降不再显著(P>0.05);还原型Vc保留率达92.54%以上,400MPa压力下保留率达95.89%;450 MPa压力下,△E~*值为0.77,很好地保持了菠萝汁原有色泽;感官分析通过定量描述分析法对不同压力处理后的样品进行分析评定,结果表明,超高压技术很好地保持了食品的色、香、味,感官品质与对照样非常接近.因此,超高压技术不仅具有较好的杀菌效果,而且较好地保持了菠萝汁的品质.%Ultra-high pressure(UHP) treatment was applied to analyze the pasteurization of pineapple juice. The changes in the physicochemical properties and sensory quality of pineapple juice were studied as well. The results showed that total number of bacteria declines with pressure (300 -500 MPa) increase and arrived commercial asepsis at 400 MPa; pH value and soluble solid content had no significant change in UHP treatment(P >0. 05) ;the turbidity decreased with pressure increased, and it changed indistinctively over 450 MPa(P >0. 05) ; the retention of antitype Vitamin C was more than 92. 54% . Under 400MPa, Vitamin C was up to 95. 89% ;△E~* value was 0. 77 at 450 MPa and the color of pineapple juice was well maintained; the results of QDA showed that the pineapple juice after UHP treatment was close to the control sample in color, flavor and taste. It is concluded that UHP treatment can effectively inactivate the microbe and maintain the quality of pineapple juice.

  3. Studies on oil emulsion inactivated vaccine against duck paramyxovirus disease%鸭副黏病毒油乳剂灭活疫苗的制备

    Institute of Scientific and Technical Information of China (English)

    刘霞; 刁有祥; 李秀娟; 李建侠

    2011-01-01

    将分离的鸭副黏病毒,经0.1%的甲醛灭活后与白油、吐温-80、司盘-80,按一定比例混合后在胶体磨中快速乳化成油包水型,制备了鸭副黏病毒油乳佐剂灭活疫苗。并对制备的疫苗进行了物理性状、无菌检验、安全性、稳定性检验、抗体消长规律、交叉免疫保护及免疫效力检验。结果表明,该疫苗安全可靠,注射后鸭的精神状况良好,饮食、粪便无异常变化,注射部位未见明显变化,疫苗吸收良好。免疫后14d即可产生抗体(平均为41og2),60d可达到91og2。免疫后25d攻毒保护率可达90%以上。%The oil emulsion vaccine against duck paramyxovirus was prepared with duck paramyxovirus isolated from duckling.duck paramyxovirus was mixed according to certain ratio with white oil,Tween-80 and Span-80 after being inactivated with 0.1% formaldehyde.And then the mixture was quickly emulsified into water in oil type by the colloid mill.The tests of the physical properties,asepsis inspection,safety test,stability test,antibody titer change rules,cross protection,immunity and effectiveness,etc.of the inactive vaccine were carried out.The results showed that the vaccine had good safety and reliability.The mental condition,eating and feces of ducks had no abnormal changes after the injection of the vaccine.The vaccine was well absorbed and the injection site was without obvious changes.The antibodies against the virus were generated at 14 d and the highest antibodies were generated 60 d after inoculation with 91og2.The protection rate could be up to 90% at 25 d after vaccination.

  4. 颅脑外伤术后医院获得性肺炎危险因素分析及防治措施%Analysis of the risk factors and preventive measurement for hospital acquired pneumonia for postoperative severe traumatic brain injury patients

    Institute of Scientific and Technical Information of China (English)

    张玉玲

    2008-01-01

    Objective To investigate the risk factors and preventive measurement of hospital acquired pneumonia(HAP) for severe traumatic brain injury patients after surgery. Methods A total of 406 cases of patients operated for severe traumatic brain injury from Oct 2004 to Sep 2007 were retrospectively analyzed, and the risk factors were summarized. Results Fifty - one out of 57 cases of patients with HAP were satisfactorily cured, the other 6 cases dead. The risk factors prone to HAP were operative anesthesia, respiratory machine application, long hospitalization, respiratory tract incision, examination, mis - inspiration, reflec-ted high hoed suger level, antibacterial use, polluted air condition etc. Conclusion Enhancing consciousness of infection can-troll, reinforcing sickroom management, keeping air condition sterilized, strictly complying with asepsis operative rules, using antibacterial correctly are significant measures to prevent HAP.%目的 探讨颅脑外伤术后医院获得性肺炎危险因素及防治措施.方法 收集我院2004年10月至2007年9月颅脑外伤术后住院患者406例,对发生医院获得性肺炎的危险因素进行回顾性分析.结果 57例医院获得性肺炎患者经积极治疗,51例治疗效果满意,死亡6例.造成医院获得性肺炎的危险因素有手术麻醉方式、接受机械通气、住院时间长、气管切开、昏迷、误吸、应激性高血糖、抗菌药物应用、空气环境污染等.结论 增强感染控制意识,加强病房管理,做好空气消毒,严格无菌操作规程,合理应用抗菌药物是预防医院获得性肺炎的重要措施.

  5. [Survey of methods of cleaning, decontamination, disinfection and sterilization in dental health services in tropical areas].

    Science.gov (United States)

    Clapeau, G; Decroix, B; Bakayoko-Ly, R; Varenne, B; Dosso-Hien, D; Decroix, M O

    1997-01-01

    hygiene standards despite difficult practice conditions, exacerbated by supply problems. In all applications, hygiene involves a succession of closely-related, logical steps, which form an asepsis chain aimed at preventing the transmission of infection. Our survey shows that fundamental elements of hygiene require attention to achieve this aim. The cleaning, disinfection and sterilizing of floor surfaces and equipment should be improved and more widespread use made of disposable items. It is important to define the hygiene level required for particular treatments, taking into account the oral and dental micro flora and whether the equipment has been decontaminated, disinfected or sterilized. A piece of equipment is decontaminated if it has been mechanically cleaned and decontaminated. It is disinfected if these steps are followed by rinsing with sterile water, drying and conditioning. An item is described as sterilized if it is cleaned, decontaminated, rinsed, dried, conditioned and then sterilized. We found that a wide variety of chemicals were used to clean hands, surfaces and equipment. The nature and appropriate methods of use of these chemicals were not widely known. Understanding the chemical composition of these chemicals makes it possible to classify them into cleaning agents, detergents, decontaminating agents and disinfectants. The definition, choice and use of antiseptics and disinfectants should be strictly controlled. It is also vital that single-use disposable items are used only once and are never reused. Hygiene in the dental surgery is a chain of processes aimed at protecting the patient and the medical staff. There are many links in the chain, involving floor and surface hygiene, hand washing by dentists and dental assistants, washing of surgery linen and treatment of equipment. Dental practitioners should continually focus on ensuring that the chain of hygiene procedures is not broken, in their own interests as well as in those of their patients.

  6. Germinação in vitro de sementes de alcachofra In vitro artichoke seed germination

    Directory of Open Access Journals (Sweden)

    Cassieli F de Moraes

    2010-03-01

    elimination; lighting conditions (light or dark; and two cultivation media [MS medium, with salts concentration reduced by half (M1 and MS medium, full strenger (M2] have been tested. In both cases, 30 g L-1 sucrose and 7 g L-1 agar were added, with pH adjusted to 5.6 with NaOH. Cultivation took place in a growth chamber. It is viable to obtain healthy artichoke plantlets in short time (seven days, to be used as a source of explants from in vitro seed germination without the tegument (77,5% of germination, using the M1 or M2 culture medium and growth chamber without light. In these conditions, the asepsis of seeds can be done with alcohol 70% during 30 minutes and the subsequent immersion in solution of 2% of active chlorine during 10 minutes, before the removal of the tegument.

  7. Legal, ethical, and procedural bases for the use of aseptic techniques to implant electronic devices

    Science.gov (United States)

    Mulcahy, Daniel M.

    2013-01-01

    animals often mask the signs of infection to avoid attracting predators (Wobeser 2006). Guidance specific to sterilization of electronic devices for implantation is limited in the wildlife record (Burger et al. 1994; Mulcahy 2003). Few biologists have been formally trained in aseptic technique, but most biologists know that electronic devices should be treated in some way to reduce the chance for infection of the host animal by bacteria, viruses, parasites, and fungi. Most biologists (73%) who implant devices into fishes believe aseptic techniques are important (Wagner and Cooke 2005). However, I maintain that many biologists find it difficult to place the concept of asepsis into practice in their work because of confusion about what constitutes aseptic technique, a lack of surgical knowledge and training, the perception of increased costs, or the belief that aseptic surgeries are impractical or unnecessary for their application. Some have even argued that, while compromising surgical techniques in the field might result in complications or mortalities, the money saved would allow for a compensatory increase in sample size (Anderson and Talcott 2006). In this paper I define aseptic surgical techniques, document the legal and professional guidance for performing aseptic surgeries on wild animals, and present options for sterilizing electronic devices and surgical instruments for field use.

  8. Assessment of protocols for surgical-site preparation in a regional network of hospitals Evaluación de la normalización de la preparación prequirúrgica en una red regional de hospitales Avaliação da normatização da preparação pré-cirúrgica em uma rede regional de hospitais

    Directory of Open Access Journals (Sweden)

    Maria Dolores Peñalver-Mompeán

    2012-04-01

    Full Text Available Surgical-site infection is a preventable adverse event. Implementation of good practices for correct surgical-site preparation can contribute to lessen this safety problem. The objective of this study was to describe the presence and quality of protocols on surgical-site preparation in the Murcia (Spain regional network of public hospitals. The indicator "existence of protocol for surgical-site preparation" was assessed, as well as the formal quality (expected attributes and contents (compared to current evidence-based recommendations of existing documents. Seven of the nine hospitals have a protocol for surgical-site preparation. Opportunities to improve have been identified in relation to the protocols' formal quality and contents. Recommendations related to skin asepsis are incomplete and those related to hair removal contradict existing evidence. Most hospitals have protocols for surgical-site preparation; however, there is great room for improvement, in relation to their expected attributes and to the inclusion of evidence-based recommendations.La infección del sitio quirúrgico es un evento adverso prevenible mediante la implementación de buenas prácticas de preparación prequirúrgica. El objetivo del presente estudio fue describir la existencia y calidad de protocolización de la preparación prequirúrgica en la red regional de hospitales públicos de Murcia (España. Se evaluó el indicador "Existencia de protocolo/norma de preparación prequirúrgica", analizando la calidad formal (atributos y de contenido (presencia de recomendaciones basadas en evidencia de los documentos existentes. Siete (de nueve hospitales acreditaron tener protocolos de preparación prequirúrgica. Existen oportunidades de mejora en la calidad formal y de contenido. Las recomendaciones sobre asepsia son incompletas en la mayoría de los documentos, y las de eliminación del vello contrarias a la evidencia. La preparación prequirúrgica está protocolizada

  9. SKIN STAPLED OR SUTURED: A COMPARATIVE STUDY

    Directory of Open Access Journals (Sweden)

    Veerendra Kumar

    2015-01-01

    Full Text Available BACKGROUND: The choice of wound closure after a surgical procedure has been a surgeon’s dilemma . The concerns have been as to how fast , uneventful , less painful , with minimal complications and comfortable will the patient’s recovery be. A well - known technology is surgical staples which are easier to use , less time consuming and a needle free method o f wound closure. OBJECTIVE: To compare skin staples with non - absorbable skin suture for skin closure in surgical laparotomy procedures for the following character : wound infection , dehiscence , cosmesis and postoperative pain . METHODS: This is a comparative study conducted on 100 patients divided in two groups randomly at Bapuji Hospital for cases undergoing surgical laparotomy procedures. Skin closure was done with 2 - 0 polyamide skin sutures in one group and skin closure was achieved with skin staples in th e other group. The outcome of wound was assessed on 3rd , 5th and 7th postoperative days using ASEPSIS score. Wound cosmesis was assessed on the 7th postoperative day and followed up at 1st month and 3rd month , using modified Hollander cosmesis scale. Postoperative pain was assessed using the visual analogue scale on the 1st , 3rd and 7th postoperative days. RESULTS : The mean wound ASPESIS scores on day 3 for skin staples and sutured group was 0.28 and 0.36 (p=0.7 respectively. The score on day 5 for staples and sutured group was 0.12 and 0.28 (p=0.4 respectively. The results in both the groups were the same , 0.08 on day 7(p=0. Wound cosmesis mean score assessment on day 7 for skin staples suturing group was 5.84 and 5.68 respectively (p=0.3. The mean score for cosmesis assessment at 1 month , and 3 rd month for skin staples and suturing group was 5.92 and 5.68 (p=0.1 and was 6.00 and 5.92 (p=0.36 respectively which was not satistically significant . The visual analogue scale score for postopera tive pain on 1st day , 3 rd and 7 th day for skin staples and suturing group 54

  10. Seletividade de Lecanicillium lecanii e Metarhizium anisopliae para larvas de primeiro ínstar de Ceraeochrysa cincta (Neuroptera: Chrysopidae em laboratório = Selectivicty of Lecanicillium lecanii and Metarhizium anisopliae for Ceraeochrysa cincta first instar larvae (Neuroptera: Chrysopidae in laboratory

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    Eliane Ribeiro Cardoso

    2007-10-01

    Full Text Available O objetivo deste trabalho foi avaliar a seletividade dos fungos Lecanicillium lecanii e Metarhizium anisopliae para larvas de Ceraeochrysa cincta, utilizando-se a aplicação por imersão e aaplicação em bases de vidro, formando filme seco (IOBC modificado. Nos dois métodos, foram aplicados os tratamentos: 1 - L. lecanii; 2 - M. anisopliae - 2.1x107 conídios viáveis mL-1; 3 - solução de Tween 80® a 0,05% como testemunha. Avaliou-se a mortalidade, duração médiado período larval, fecundidade e a viabilidade dos ovos. Todos os insetos mortos foram submetidos à assepsia, para confirmação da mortalidade causada pelo fungo. Em ambos os bioensaios, as mortalidades confirmadas pelos fungos foram iguais a zero. No filme seco o efeito total dos fungos sobre o predador foi classificado como pouco nocivo de acordo com as categorias propostas pelo método IOBC. L. lecanii e M. anisopliae não afetaram a viabilidade dos ovos de C. cincta, quando aplicados por imersão. Na concentração que foi estabelecida para a realização dos bioensaios, os fungos entomopatogênicos L. lecanii e M. anisopliae foram seletivos para larvas de 1o ínstar de C. cincta.This paper aimed to evaluate the selectivity of the fungi Lecanicillium lecanii and Metarhizium anisopliae for Ceraeochrysa cincta. Two bioassays were developed: 1st application of dry filmproposed by the International Organization for Biological Control IOBC (modified - they were applied in glass bases, forming dry film. 2nd immersion application. In the two methods, the following treatments were applied: 1- watery suspension of L. lecanii; 2- the same for M. anisopliae (both at 2.1x107 viable conidia mL-1; and 3- Tween 80® 0.05% watery solution. The larvae mortality, the average duration of the larvae period, the females fecundity and the eggsviability were evaluated. All the dead insects were submitted to asepsis to confirm the mortality caused by the fungi. In both bioassays, the mortality

  11. Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii=Micropropagação de um híbrido de Eucalyptus (Eucalyptus benthamii x Eucalyptus dunnii.

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    Fabricio Augusto Hansel

    2011-10-01

    Full Text Available This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP and naphthaleneacetic acid (NAA for bud proliferation, and the ratio between BAP and gibberellic acid (GA3 in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1 of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1 and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1 for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1 and GA3 (0, 0.1, 0.2 and0.3 mg L-1. The 0.50 mg L-1 BAP and 0.050 mg L-1 NAA combination was optimal for bud proliferation for H12 and H20. GA3 concentrations of 0.10 and 0.20 mg L-1 combined with 0.10 mg L-1 BAP on ½MS resulted in the longest shoots, for H12 and H20, respectively. Regardless of clone, the rooting rate was low, with an average of 12.0% and 14.4% of plants having roots for in vitro and ex vitro conditions, respectively.Objetivou-se micropropagar E. benthamii x E. dunnii, testando concentrações de cloro para a assepsia de explantes, a concentração ótima de benzilaminopurina (BAP e ácido naftalenoacético (ANA para a proliferação de gemas e a relação entre BAP e ácido giberélico (GA3 em dois meios de cultura para o alongamento de brotações. Segmentos nodais dos genótipos H12, H19 e H20 foram desinfetados com 0,5; 1,0; 1,5 e 2,0% (v v-1 de cloro. Os explantes foram multiplicados em meio ½MS suplementado com BAP (0; 0,25; 0,50; 0,75 e 1,00 mg L-1 e ANA (0; 0,025; 0,050; 0,075 e 0,100 mg L-1 para produção de gemas, e alongados nos meios MS e ½MS suplementados com BAP (0; 0,05 e 0,10 mg L-1 e GA3 (0; 0,1; 0,2 e 0,3 mg L-1. A combinação de 0,50 mg L-1 de BAP e 0,050 mg L-1 de ANA proporcionou melhor proliferação de gemas para os gen

  12. Incidence of seedborne fungi on pink trumpet tree (Tabebuia impetiginosa and yellow poui (Tabebuia ochracea in Roraima = Incidência de fungos associados a sementes de ipê-rosa (Tabebuia impetiginosa e ipê-amarelo (Tabebuia ochracea em Roraima

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    Jefferson Fernandes do Nascimento

    2012-04-01

    Full Text Available The objective of this study was to identify and quantify fungi associated with seeds ipe-pink (Tabebuia impetiginosa, yellow-ipe (Tabebuia ochracea and evaluate the effects of aseptic seed germination and seed seeding. The experiment was done at the Laboratory of Plant Science Center of Agrarian Sciences, Federal University of Roraima. Samples of ipê yellow and pink ipe were collected and extracted from fruits harvested in the urban area of the city of Boa Vista - RR. Each sample was divided into two sub-samples, treated and untreated, and were then submitted to germination and health analyses. The filter paper method was used for the health test where contaminated seeds were analyzed for the detection and identification of fungi. For the germination test method was also the filter paper, obtained the germination rate, rate of plants with lesions, number of seeds germinated and the rate of non-germinated seeds that had fungus. The results were subjected to variance analysis and when significant, means were compared by the Tukey test at 5% probability. The experimental design was completely randomized with four replications of 50 seeds for each treatment. Asepsis with 70% alcohol for a minute then with sodium hypochlorite (NaClO to 2% for three minutes was positive for the elimination of fungi on seeds of ipe-yellow and ipe-pink, it favored germination and decreases the rate of seedlings with lesions. = Objetivou-se com o presente trabalho identificar e quantificar os fungos associados às sementes de ipê-rosa (Tabebuia impetiginosa, ipê-amarelo (Tabebuia ochracea e avaliar o efeito da assepsia das sementes na germinação e na plântula. O Experimento foi realizado no Laboratório de Fitotecnia do Centro de Ciências Agrárias da Universidade Federal de Roraima. Foram coletadas amostras de sementes de ipê-amarelo e ipê-rosa extraídas de frutos colhidos na área urbana do Município de Boa Vista - RR. Cada amostragem foi dividida em duas

  13. Estabelecimento, multiplicação e alongamento in vitro de Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden Establishment, multiplication and elongation in vitro of Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden

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    Gilvano Ebling Brondani

    2009-02-01

    Full Text Available Neste trabalho foram testadas diferentes concentrações de cloro ativo (NaOCl na assepsia de explantes para o estabelecimento in vitro, bem como benzilaminopurina (BAP e ácido naftalenoacético (ANA para a multiplicação e alongamento de Eucalyptus benthamii x Eucalyptus dunnii. As minicepas fornecedoras de propágulos para introdução in vitro foram conduzidas em minijardim clonal sob sistema semi-hidropônico. Segmentos nodais dos clones H12, H19 e H20 foram desinfestados com 0,5; 1,0; 1,5; e 2,0% (v/v de cloro ativo durante 10 min e inoculados em meio de cultura MS. Na obtenção de brotações múltiplas, utilizou-se o meio de cultura ½MS suplementado com 0; 0,25; 0,50; 0,75; e 1,0 mg L-1 de BAP. Na fase de alongamento, utilizou-se o meio de cultura ½MS com 0; 0,25; 0,50; 0,75; e 1,0 mg L-1 de ANA. Não houve interação entre os fatores estudados, obtendo-se 45%, 46% e 66% de estabelecimento do clone H12, H19 e H20, respectivamente. A concentração de BAP que resultou na maior proliferação de gemas axilares para o clone H12 aos 60 dias foi estimada na faixa de 0,25 e 0,30 mg L-1. Aos 60 dias, a faixa entre 0,25 e 0,75 mg L-1 de ANA promoveu o maior número de brotações alongadas do clone H12.This work aimed to evaluate different concentrations of active chlorine (NaOCl in explant asepsis for the establishment in vitro as well as of benzylaminopurine (BAP and naphthalene acetic acid (NAA in the multiplication and elongation of Eucalyptus benthamii x Eucalyptus dunnii. Ministumps, which supply shoots for introduction in vitro, were cultivated in a clonal mini garden under semi-hydroponic system. Nodal segments of clones H12, H19 and H20 were disinfested with 0.5, 1.0, 1.5 and 2.0% (v/v of active chlorine during 10 minutes and inoculated in MS medium. In the multiplication phase, culture ½MS medium supplemented with 0, 0.25, 0.50, 0.75 and 1.0 mg L-1 of BAP was used. In the elongation phase, ½MS medium supplemented with 0, 0.25, 0

  14. TO COMPARE THE EFFICACY OF INTRAMUSCULAR PHENYLEPHRINE AND EPHEDRINE IN LSCS T O AVOID POST SPINAL HYPOTENSION

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    Jaideep

    2015-03-01

    Full Text Available INTRODUCTION: Hypotension during Spinal Anesthesia Subarachnoid block for LSCS is secondary to the sympathetic blockade and aorto - caval compression by the uterus and it can be deleterious to both fetus and mother. Ephedrine and phenylephrine improve venous return after sympathetic blockade during Subarach noid block. AIMS AND OBJECTIVES: The objective of this study was to assess the role of Intramuscular phenylephrine and ephedrine and to compare the efficacy of Intra muscular Phenylephrine and ephedrine in LSCS to avoid post spinal hypotension. MATERIALS A ND METHODS: Ninty patients undergoing subarachnoid block with 0.5% Bupivicaine heavy for LSCS in Left Lateral Position in L 3 - L 4 Interspinous space via 25 G Spinal Needle under full asepsis were randomly divided into 3 groups. GROUP A: Received neither i.m. phenylephrine nor i.m. ephedrine. Only Co - loading with crystalloid. GROUP B: Received intramuscular ephedrine (25 mg 10 minute prior to spinal anaesthesia & Co - loading with crystalloid. GROUP C : Received intramuscular phenylephrine (2 mg 10 minute prior to spinal anaesthesia. & Co - loading with crystalloid. PATIENT SELECTION CRITERIA : INCLUSION CRITERIA: Full Term Pregnancy, ASA Grade - I and Grade – II . EXCLUSION CRITERION: Contraindications for spinal block, coagulopathies, eclamptic and preeclampt ic patient. INVESTIGATION REQUIRED : Routine Investigation , CBC , PTT, APTT, INR, Urine Investigation . DISCUSSION : Phenylephrine and ephedrine are comparable vasopressors when used to treat hypotension during caesarian section after spinal anaesthesia. Signi ficant difference in HR between groups can primarily attributed to the decline in HR observed with phenylephrine and increase i n HR associated with Ephedrine. The incidence of tachycardia was significantly higher in Ephedrine group due to its B1 - agonist pr operty . Furthermore, the incidence of fetal tachycardia with Ephedrine was more significant in another study

  15. Crescimento inicial de mangabeira (Hancornia speciosa Gomes em diferentes meios de germinação in vitro Initial growth of mangabeira (Hancornia speciosa Gomes in different culture medium of in vitro germination

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    Ana da Silva Lédo

    2007-08-01

    Full Text Available O objetivo do trabalho foi determinar as condições mais favoráveis para a germinação in vitro de sementes e o crescimento inicial de plântulas de mangabeira. Após assepsia, sementes oriundas de frutos maduros foram inoculadas em tubos de ensaio contendo os seguintes tratamentos: T1-15 mL de meio de cultura MS; T2-15 mL de meio de cultura MS + 2,0 g L-1 de carvão ativado; T3-15 mL de meio de cultura ½ MS; e T4-15 mL de meio de cultura ½ MS + 2,0 g L-1 de carvão ativado. Todos os meios de cultura foram gelificados com 0,3 g L-1 de Phytagel® e suplementados com 3,0 g L-1 de sacarose. O experimento foi instalado em delineamento inteiramente casualizado com quatro tratamentos e oito repetições, sendo cada parcela experimental composta de dez tubos de ensaio contendo uma semente cada. Não houve diferença significativa dos tratamentos para a porcentagem de germinação aos 20 dias, que variou de 95 a 100%. Quanto ao comprimento da raiz principal, observou-se que o meio de cultura constituído de ½ MS com 2,0 g L-1 de carvão ativado proporcionou maior crescimento quando comparado com os demais tratamentos. Aos 50 dias, não foi observada a formação de plântulas anormais e nem diferenças significativas do comprimento da parte aérea das plântulas. Entretanto, a diluição em 50% dos sais do meio MS associada à presença de carvão ativado induziu maior crescimento da raiz principal (8,50 cm quando comparado com meio MS, na presença (6,19 cm ou ausência (6,00 cm de carvão ativado.The objective of this study was to determine the most favorable conditions for the in vitro germination of mangaba seeds and initial development of plantlets. After asepsis, emerging seeds of mature fruits were inoculated in tubes contend the next treatments: T1-15 mL of MS culture medium; T2-15 mL of MS culture medium + 2.0 g L-1 of activated charcoal; T3-15 mL of ½ MS culture medium; and T4-15 mL of ½ MS culture medium + 2.0 g L-1 of activated

  16. Infecciones de repetición en un paciente portador de un sistema de neuromodulación tras la picadura de insecto Recurrent infection following an insect bite. In a patient with a neuromodulation system

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    V. Villanueva

    2006-06-01

    cord stimulators for the management of severe and intractable pain. Complications after the implantation of the electrodes are infrequent, but some as breakage, infection and displacement have been reported. The case of a young technical agricultural engineer with a medical history of allergy to acarus is described. He developed a septic shock following the sting of an insect (Simu-lium Damnasum on the top right limb, being admitted to intensive care for 48 hours. During his hospital stay, he had severe neuropathic pain with a poor response to conventional treatment. Three and a half years after this event, the patient was sent to the Pain Management Unit, where the first stage of an electrode implantation for a spinal cord stimulator was carried out. The system was withdrawn after six days due to a Staphylococcus Aureus infection. A series of epicutaneous test for regular substances and for those from the spinal cord stimulator components were made. The likelihood of an allergy to some component of the implantable device, responsible for an inflammatory reaction and subsequent infection of the catheter, was ruled out. It is important to point out that careful asepsis is still the best means to avoid infections. In patients with a medical history of atopia or allergic reactions, allergic tests should be considered as a measure to prevent rejections of devices.

  17. 花烛突变体叶色嵌合性状的分化特征与保持方法%Differentiation characteristics and retention method of leaf color chimera feature of mutated plantlet of Anthurium andraeanum

    Institute of Scientific and Technical Information of China (English)

    陈星旭; 邵会会; 王广东

    2011-01-01

    Using asepsis seedlings of leaf color chimera type of Anthurium andraeanum ' Sonate' as the experimental materials, single-bud cultures of sixteen individuals without top bud chimera feature were carried out, and differentiation characteristics of leaf color chimera feature during regeneration process of axillary bud and stem basal part were observed. And based on observation results, the retention method of leaf color chimera feature of mutated plantlet of A. Andraeanum was summed up. The results show that chimera percentage of regenerated plantlets from axillary buds of mutated plantlets in proliferation and rooting phases is 25.0%-75.0% and 25.0%-66.7% and total chimera percentage is 48.4% and 47. 8% , respectively. The regenerated plantlets from axillary buds with chimera feature are all burgeoned from axils of chimera leaves. And chimera plantlet can also be regenerated from the stem basal part of mutated plantlets in rooting phase with a chimera percentage of 33. 3% -80. 0% and total chimera percentage of 64. 7%. It is concluded that the problem of losing leaf color chimera feature of top bud of mutated plantlet of A. Andraeanum could be resolved by single-bud in vitro culture. And chimera feature of mutated plantlet can be retained with axillary bud (in proliferation and rooting phases) and basal part (in rooting phase) regeneration cultures.%以花烛品种‘Sonate’(Anthurium andraeanum‘Sonate’)的叶色嵌合型无菌苗为材料,对顶芽失去嵌合性状的16个单株分别进行单芽培养,观察侧芽及茎基部再生过程中叶色嵌合性状的分化特征;并据此归纳花烛突变体叶色嵌合性状的保持方法.结果表明:处于增殖和生根阶段的花烛突变单株侧芽再生植株的嵌合率分别为25.0%~75.0%和25.0% ~ 66.7%,总的嵌合率分别为48.4%和47.8%,具有嵌合性状的侧芽再生植株均萌发于嵌合叶片的叶腋处;处于生根阶段的突变单株的茎基部也能再生出

  18. 综合教学管理方式在手术室中等护理毕业实习生中的应用%Application of comprehensive teaching management for secondary nursing graduate stduents in operating room

    Institute of Scientific and Technical Information of China (English)

    陈慧豪; 肖大伟

    2015-01-01

    could improve operation room secondary nursing graduate trainee self-confidence,personal asepsis consciousness and ability to work independently,improve the clinical nursing teaching job satis-faction,reduce the occupation exposure rate,improve the overall quality of teaching and intern operation room comprehensive quality.

  19. Analysis of bacterial culture test among three gloves wearing techniques%三种戴手套法的无菌效果分析

    Institute of Scientific and Technical Information of China (English)

    周晓虹; 谭丽; 何思勤; 谭吉林

    2015-01-01

    目的:对比分析三种戴手套法即传统戴手套法、半封闭式戴手套法、全封闭式(非接触式)戴手套法的无菌效果。方法选3名手术室护士,先按外科手消毒规范洗手,穿无菌衣,用碘伏擦手消毒,然后分别用三种方法各戴手套10双,戴手套时发现有向内卷边者或戴手套后发现手套外粘有碘伏者视之为污染,并用棉试子涂抹采样作细菌培养。结果传统戴手套法:60只手套中有21只污染,占35%;半封闭式戴手套法:60只手套中有1只污染,占1.67%;全封闭式戴手套法:60只手套中有1只污染占1.67%。半封闭式和全封闭式戴手套法与传统戴手套法相比,其差异均具有统计学意义(χ2=22.26,P<0.01)。细菌培养结果:在传统戴手套法中有1只手套取样发现细菌生长。结论半封闭式和全封闭式戴手套法,均有效避免了传统戴手套法易卷边污染无菌面的缺点,能有效减少感染机会;其中半封闭式戴手套法其戴手套方式类同传统戴手套法,操作更简单,更易推广。%Objective Contrastive analysis of bacterial culture test among three techniques of wearing opera‐tion gloves :traditional ,semi‐closed ,full‐closed (non‐contacted) .Methods Selected five operation room nurses ,hand‐washing with traditional surgical hands disinfection standard ,wearing asepsis clothing ,and disinfected with povidone‐iodine ,then wearing 10 pairs of operation gloves respectively by above three techniques ,contamination was deter‐mined while inward curling of glove edge or povidone‐iodine was found outside of the gloves ,take cotton‐swab smear sampling method for the germiculture .Results Traditional method :21 of 60 (35% ) gloves were found polluted ;Semi‐closed method :1 of 60 (1 .67% ) gloves was polluted ;full‐closed method :1 of 60 (1 .67% )gloves were pollu‐ted .The difference between the traditional and

  20. Effects of quorum sensing system lasR/rhlR gene on the expression of Foxp3, TGF-β1 and IL-10 of lung tissue in tracheal intubation model rat with Pseudomonas aeruginosa biofilm infection

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    Qing-qing XIANG

    2016-03-01

    Full Text Available Objective  To investigate the effects of lasR/rhlR gene on Foxp3, TGF-β1 and IL-10 of lung tissue in rat tracheal intubation model with biofilm infection of Pseudomonas aeruginosa (Ps. aer wild strain (PAO1 and quorum sensing (QS deficient strain (ΔlasRΔrhlR. Methods  Twenty-one SD rats were randomly assigned into 3 groups (7 each: ΔlasRΔrhlR-treated group, PAO1-treated group and sterile control group. Biofilms (BF were cultured in vitro, and the BF coated tube (infected respectively with Ps. aer PAO1 strain, ΔlasRΔrhlR strain, or with asepsis was inserted into the trachea to establish the rat model. The rats were sacrificed on the 7th day after intubation. Colony count of lung tissue homogenate (cfu and lung HE staining were performed, and IL-10 content in bronchoalveolar lavage fluid (BALF, TGF-β1 in lung tissue, and the expression of Foxp3 mRNA in lung cells were determined. Results  The bacterial counts were significantly higher in PAO1 and ΔlasRΔrhlR groups than that in sterile control group, and the counts were obviously higher in PAO1 group (10 400.00±6313.70/g lung tissue than that in ΔlasRΔrhlR group (975.00±559.97/g lung tissue, P<0.05. There was no significant pathological changes in lung tissue in sterile control group, while the bronchi and blood vessels in PAO1 group were infiltrated by a large number of inflammatory cells and complicated with alveolar septum thickening and local abscess and necrosis. The pathological changes were milder in ΔlasRΔrhlR group than in PAO1 group; the expression of Foxp3 mRNA was higher in the two Ps. aer infected groups than that in sterile control group (0.65±0.32, and it was significantly higher in PAO1 group (4.62±1.07 than in ΔlasRΔrhlR group (2.15±1.43, P<0.05. The accumulated optical density value of TGF-β1 was significantly higher in the two Ps. aer infected groups than in sterile control group (3721.66±1412.95, and significantly higher in PAO1 group (65 090.56±33

  1. 老年患者真菌感染的临床特点和耐药性分析%Clinical ditribution and drug resistance of infection in senile patients

    Institute of Scientific and Technical Information of China (English)

    陈雪芳; 王佳良

    2011-01-01

    Objective:To investigate the clinical character and drug resistance of infection in senile patients. Methods: The related clinical datas and results of drug sensitivity tests of candida infection in hospitalized senile patients isolated from January 2007 to December 2009 were analyzed retrospectively. Results: Four hundred and forty one strains of fungi were isolated from 377 patients specimen and most of them were Candida Spp. (94.8%) ,the rest were aspergillus and mucorpusillus (5.2%). The departents ofrespiratory and ICU had the top relevance ratio which were (29.3%)和( 25.4%. )Most fungi were found in sputum (47.2%) and urine(23.1% ) got from patients. There had a highest susceptibility rate to amphoterien B and 5 - fluoruytosine according to drug sensitivity tests in vitro. Within eriazole antifurgals, the resistante rate of candida to voriconazole was low, and nevertheless, the resistante rate of non - candida albicans was high. Conclusion: Candida has been one of the most common pathogens proceed from senile patient nosocomial infection, and that drug resistance is increasing. Senile patients are high risk group and attention are necessary to whose physical symptom change. Strict standards of asepsis operation will help to reduce the incidence rate of fungi infection.%目的:了解老年患者真菌感染的临床特点.并分析其耐药性.方法:回顾性调查2007年1月-2009年12月住院的老年患者发生真菌感染的相关临床资料及药敏试验结果.结果:377例患者标本共分离真菌441株.菌种以假丝酵母菌为主,占94.8%,曲霉菌和毛霉菌占5.2%.呼吸内科和重症监护室的检出率最高,分别为29.3%和25.4%.痰液和尿液是检出菌株数最多的标本,构成比分别占47.2%和23.1%.体外药敏试验结果表明.两性霉素B和5-氟胞嘧啶的敏感性最高.三唑类抗真菌药物中.以伏立康唑耐药率较低,氟康唑对非白假丝酵母菌耐药性较强.结论:假丝酵母菌是老

  2. Establishment of a new continuous culture and multiple harvests processusing cell factory for rabies vaccine for human use%应用细胞工厂建立人用狂犬病疫苗连续培养多次收获工艺

    Institute of Scientific and Technical Information of China (English)

    李红芳; 徐枫; 赵祖波; 任红卫; 杨淼; 马超; 辛军; 龚健; 李守丽; 于滢

    2013-01-01

    目的:为提高生产效率、增加原代地鼠肾细胞单产量及狂犬病病毒产量,建立人用狂犬病疫苗(地鼠肾细胞)连续培养多次收获工艺。方法选用12~14日龄SPF地鼠,无菌取肾经消化,制备成细胞悬液,分装到40层细胞工厂并培养细胞成单层;接种狂犬病病毒固定毒aG株,连续培养病毒并多次收获。分别对同一细胞批制备的多个单次病毒收获液的免疫原性、病毒滴度和地鼠肾细胞蛋白质含量进行检测。结果用40层细胞工厂培养原代地鼠肾细胞和狂犬病病毒,细胞接种浓度为1.0×106~1.5×106cells /mL,(36±1)℃培养72 h成致密单层;按0.1 MOI病毒接种,可进行6次收获病毒;多个单次病毒收获液病毒滴度均不低于6.0 lgLD50/mL;免疫原性检查保护指数不低于100;地鼠肾细胞蛋白质残留量随着收获次数的增加而不断降低。结论用细胞工厂建立了人用狂犬病疫苗连续培养多次收获工艺,能显著提高地鼠肾单产量,增加产能。%Objective To improve the production efficiency and increase primary hamster kidney cells ( PHKC) and rabies virus yield.To establish a new continuous culture and multiple harvests process using cell factory for rabies vaccine ( Ham-ster kidney cells ) for human use .Methods PHKC suspensions were prepared by asepsis digesting 12 to 14 day old pri-mary hamster kidney .The cells suspension was packed into cell factory and cultured to cell monolayer .Rabies fixed strains aG was inoculated and cultured ,and then multiple harvests were taken .Immunogenicity test , virus titer test and PHKC matrix proteins tset were taken for every single virus harvest from one cell batch .Result Cells cultures were carried out in CellSTACK®-40(40-layers cell factory) with cell density of 1.0×106-1.5×106cells /mL at (36±1) ℃ for 72 h.Cells were infected with rabies aG strains at 0.1 MOI and six virus harvests were

  3. 糖尿病视网膜病变光凝术的并发症与处理%The mechanism and treatment of laser complication in diabetic retinopathy

    Institute of Scientific and Technical Information of China (English)

    张福燕; 李志敏; 李建阳; 李青; 陈玲玲; 吴端华

    2009-01-01

    .58%) (2 eyes with further photocoagulation, 6 eyes underwent vitrectomy, 2 eyes gave up treatment);9 eyes (6.82%) were dazzled and this disappeared alter 20 minutes spontaneously;4 eyes (3.03%) had acute conjunctivitis, strict surgical asepsis during laser surgery and routinely use antibacterial eye drops were recommend; 3 eyes (2.27%) had acute elevating of intraocular pressure and blood pressure with light perception disappeared.The visual acuity recovered after lowering the intraocular pressure and blood pressure; 1 eye had ciliary body and choroid edema with anterior chamber disappeared, pupil adhesion and second glaucoma.One patient had hypoglycemia and became safe after emergency treatment.Conclusions Master the technical skill of panreteinal photocoagulation to DR and thoroughly understand the mechanism and treatment of laser complication is very important to reduce the incidence of complication and avoid the serious consequences.

  4. Medida do volume da úlcera cutânea na leishmaniosetegumentar

    Directory of Open Access Journals (Sweden)

    Dalmo Correia

    1996-12-01

    skin surface transverse and longitudinal diameters. The proposal is to mould the cavity, after local asepsis with fresh water plus soap, with a gelatinous plastic which contains silice, potassium alginate, calcium sulphate,magnesium oxide commercialized under the name of jeltrate® (Dentsply Laboratory, by solving 9-5g of jeltrate® in 20ml of fresh water and applying the gel on the ulcer which solidifies in 5 minutes. This mould is then filled with a self polymerising aciylic and its volume measured either by weight (by using an analytical balance - technique 1 - or by water displacement by applying Archimeds'principle - technique 2. We shoiu data in a field trial before and after 20 days treatment in 20 patients using three different schedules as follows: 7 received pentamidine isethionate, 7 patients received aminosidine sulphate and 6 received meglumine antimoniate. Tloe results point out that there was a uniforme reduction of ulcer volume occurred during this period in the three groups, in both technique. Regarding the therapeutic schedules we are sure that there was a significant statistical difference between the three schedules using the TStudent Test, which showed that aminosidine sulphate produced a better volume reduction of the ulcer than the other drugs. Serial moulds reflect clinical healing and ai'e a permanent record. We conclude that the measure of the volume of the skin ulceration can be usefull in the therapeutic evaluation, as a practical and cheap procedure, and 7nay be used in field trials..

  5. Elimination of intracanal infection in dogs' teeth with induced periapical lesions after rotary instrumentation: influence of different calcium hydroxide pastes Eliminação da infecção intracanal em dentes de cães com lesões periapicais induzidas após instrumentação automatizada: influência de diferentes pastas de hidróxido de cálcio

    Directory of Open Access Journals (Sweden)

    Janir Alves Soares

    2006-06-01

    Full Text Available The aim of this study was to evaluate the antiseptic efficacy of rotary instrumentation associated with calcium hydroxide-based pastes prepared with different vehicles and antiseptics. Chronic periapical lesions were experimentally induced in 72 premolar root canals of four dogs. Under controlled asepsis, after initial microbiological sampling (A1, the root canals were instrumented using the ProFile system in conjunction with 5.25% sodium hypochlorite and the intracanal medication was placed. Four experimental groups were formed according to the pastes used: group 1- Calen (n=18, group 2- Calen+CPMC (n=20, group 3- Ca(OH2 p.a.+ anaesthetic solution (n=16 and group 4- Ca(OH2 p.a.+ 2% chlorhexidine digluconate (n=18. After 21 days, the pastes were removed; the canals were emptied and 96 hours later a second microbiological sample was obtained (A2. The incidence of positive microbiological cultures and the number of cfus in stages A1 and A2 were compared statistically by the Wilcoxon test while the influence of the different treatments in intracanal infection was evaluated by Kruskal-Wallis test at 5% significance level (pO objetivo deste estudo foi avaliar a eficácia anti-séptica da instrumentação rotatória associada às pastas à base de hidróxido de cálcio [Ca(OH2] contendo diferentes veículos e anti-sépticos. Lesões periapicais crônicas foram experimentalmente induzidas em 72 canais radiculares de pré-molares de 4 cães. Sob controlada assepsia, após amostras microbiológicas iniciais (A1, fez-se a instrumentação com o sistema ProFile coadjuvado pela solução de hipoclorito de sódio a 5,25%, seguido de medicação intracanal. Em função das pastas utilizadas obtiveram-se 4 grupos: grupo 1- Calen (n=18, grupo 2- Calen+PMCC (n=20, grupo 3- Ca(OH2 p.a.+ solução anestésica (n=16 e grupo 4- Ca(OH2 p.a.+ solução de digluconato de clorexidina a 2% (n=18. Transcorridos 21 dias, removeram-se as pastas, deixando os canais

  6. 2011年黑龙江省黑瞎子岛鼠群中钩端螺旋体感染状况调查%The infection status of Leptospira in rodents on the Heixiazi island of Heilongjiang province, China,in 2011

    Institute of Scientific and Technical Information of China (English)

    王振东; 王莎莎; 刘丽娟; 杨宇; 李明; 郭天宇; 富英群; 侯咏; 孙肖红

    2013-01-01

    目的 调查2011年黑龙江省黑瞎子岛鼠群中钩端螺旋体的感染情况.方法 于2011年4-10月在黑龙江省黑瞎子岛采用夹夜法捕鼠,共捕鼠356只,无菌操作采集鼠肾样品;提取鼠肾脏总核酸,采用PCR方法,利用钩体鉴别引物,对提取的肾脏总核酸中23 S rDNA的482 bp特异性片段进行扩增,以检测鼠体钩端螺旋体感染情况;抽取不同月份的15份阳性扩增产物进行纯化,用双脱氧终止法测序,并利用Mega 4.0软件与Genebank中已知的国内外菌株基因序列进行比对及系统发育分析.结果 在捕捉到的356只鼠中,红背(鼠平)和黑线姬鼠所占比例较大,分别为39.3%(140/356)和36.0%(128/356).所有鼠中共检测到钩端螺旋体阳性39只,感染率为11.0%,除褐家鼠外,其他鼠种均有感染,黑线姬鼠、红背鼾、东方田鼠、花鼠和大林姬鼠的感染率分别为9.4% (12/128)、12.9%(18/140)、10.8%(7/65),差异无统计学意义(x2=1.92,P>0.05).4月份未检测到感染疫鼠,5、6、7、8、9和10月份的感染率分别为5.6%(4/72)、8.8%(5/57)、12.8%(5/39)、9.8%(5/51)、33.3%(11/33)和22.5% (9/40),差异有统计学意义(x2=32.92,P<0.05);黑瞎子岛鼠群感染的钩端螺旋体在同一进化支上,相似度为97.1%~99.6%,与澳大利亚的菌株U90865在进化上最为相近,相似度>96.3%.结论 该地区鼠群中已存在钩端螺旋体的感染,所感染钩体在进化上相似,且存在季节性差异,而对宿主的选择性不明显.%Objective To study the infection status of Leptospira in rodents on Heixiazi island Heilongjiang province in 2011.Methods A total of 356 rodents were captured by night trap on the Heixiazi island from April to October 2011.The kidney tissue samples were collected by asepsis operation and the genomic DNA were extracted from them.Leptospira strains were confirmed by polymerase chain reaction (PCR) amplification of the 482 bp 23 S rDNA gene.Fifteen PCR

  7. Study of VEGF transfection on rabbit mesenchymal stem cells%VEGF基因修饰兔骨髓间充质干细胞的研究

    Institute of Scientific and Technical Information of China (English)

    徐松柏; 赵刚; 赵红光; 许侃; 于洪泉; 候宜

    2008-01-01

    BACKGROUND: Vascular endothelial growth factor (VEGF) is a very effective way to make tissue engineer bone vascularization.However, because of expensive and short half-life, VEGF cannot maintain effective concentration in blood after injection. To resolve the problem effectively, gene transfection technique is used in this experiment to transfer human VEGF into seed cells-mesenchymal stem cells (MSCs) of tissue engineer bone and to make it secrete VEGF which could vascularize bone.OBJECTIVE: To explore the possibility of human vascular endothelial growth factor 165 (VEGF165) to transfect rabbit MSCs, and establish the experimental foundation of angiogenesis tissue engineering organization and the treatment of ischemic disorders.DESIGN: Observation control trail.SETTING: First Hospital of Jilin University and Institute of Frontier Medical Sciences of Jilin University.MATERIALS: The experiment was conducted in the Key Laboratory (BSL-2) of Frontier Medical Sciences of Jilin University between June 2003 and August 2004. Health New Zealand white rabbits, 4.0-5.0 months old, weighing 2.5-3.5 kg, half male and half female, were provided by Animal Center of Jilin University. The rabbits were handled under asepsis and anesthetized condition,corresponding to the animal ethical standard. Medicine and reagents: Ham F12 culture media (Gibco, U.S), MTT (Sigma, U.S)PLXSNKDRp-VEGF165 and pcDNA 3.0 vectors were prepared in the present laboratory. ELISA detection kit (Jingmei company,Shenzhen), DH5 α, restriction endonucleases Barn H I, Xhol Ⅰ, Hind Ⅲ, EcoR Ⅰ and standard DNA molecule (Promega,U.S) were also used in this study.METHODS: Rabbits' MSCs were separated and cultivated. The pcDNA 3.0-hVEGF165 expression vector was constructed and identified, pcDNA3.0-VEGF165 eukaryotic expression vector was constructed, the vector was used directly to transfect MSCs. The cultural supernatant then was collected and the soluble protein of human VEGF gene expression was analyzed with