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Sample records for articular inflamatoria del

  1. Estudio del metabolismo mineral y óseo en pacientes pediátricos afectos de enfermedad inflamatoria intestinal

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    Aguilar Quintero, María

    2014-01-01

    INTRODUCCIÓN Y MOTIVACIÓN DE LA TESIS En los últimos años ha aumentado el interés por conocer las alteraciones del metabolismo osteomineral en los pacientes afectos de enfermedad inflamatoria intestinal (EII), debido entre otras razones a que un gran número estos pacientes presentan una enfermedad metabólica ósea como epifenómeno de su proceso de base. Así, se estima que la prevalencia de EII subyacente estaría en torno al 40-50% en caso de osteopenia y al 5 al 30% en caso de o...

  2. Estudio de la respuesta inflamatoria del ácido poliláctico

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    Josa, Célia

    2011-01-01

    Doble titulació El ácido poliláctico (PLA) es un polímero biodegradable ampliamente utilizado en la fabricación de dispositivos médicos y más recientemente en la fabricación de andamios para ingeniería de tejidos. Una de las respuestas desencadenadas al implantar un biomaterial en el cuerpo es la respuesta inmune. Esta puede conllevar a su vez a la respuesta inflamatoria que es llevada a cabo principalmente por monocitos que posteriormente se transforman en macrófagos. Los macr...

  3. Pharmacological Nutrition in Inflammatory Bowel Diseases Nutrición farmacológica en las enfermedades inflamatorias del instestino

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    F. G. Campos

    2003-04-01

    Full Text Available Inflammatory Bowel Diseases - ulcerative colitis and Crohn’s disease- are chronic gastrointestinal inflammatory diseases of unknown etiology. Decreased oral intake, malabsorption, accelerated nutrient losses, increased requirements, and drug-nutrient interactions cause nutritional and functional deficiencies that require proper correction by nutritional therapy. The goals of the different forms of nutritional therapy are to correct nutritional disturbances and to modulate inflammatory response, thus influencing disease activity. Nutritional intervention may improve outcome in certain individuals; however, because of the costs and complications of such therapy, careful selection is warranted. Total parenteral nutrition has been used to correct and prevent nutritional disturbances and to promote bowel rest during active disease, mainly in cases of digestive fistulae with a high output. Its use should be reserved for patients who cannot tolerate enteral nutrition. Enteral nutrition is effective in inducing clinical remission of disease in adults and promoting growth in children. Recent research has focused on the use of specific nutrients as primary treatment agents. Although some reports have indicated that glutamine, short-chain fatty acids, antioxidants and immunonutrition with omega-3 fatty acids are an important therapeutic alternative in the management of inflammatory bowel diseases, the beneficial reported effects have yet to be translated into the clinical practice. The real efficacy of these nutrients still need further evaluation through prospective and randomized trials.Las enfermedades inflamatorias del intestino -colitis ulcerosa y enfermedad de Crohn- son enfermedades crónicas de causa desconocida. La disminución de la ingesta, la malabsorción, la pérdida acelerada de nutrientes, el aumento de los requerimientos y las interacciones entre medicamentos y nutrientes determinan carencias nutricionales y funcionales que obligan a una

  4. Rescate articular con colgajo anterolateral del muslo

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    F.J. García-Bernal

    2015-06-01

    Full Text Available La preservación del nivel de amputación tanto de la extremidad superior como de la inferior, puede mejorar la función residual y el proceso de rehabilitación protésica de la misma. Ambas van a estar condicionadas por la longitud restante de la extremidad, la presencia o no de articulaciones operativas y la calidad de la cobertura del muñón. Presentamos 2 casos de amputaciones. El primero es un caso agudo de amputación traumática de extremidad superior a la altura del tercio proximal de antebrazo, que asocia avulsión cutánea circular desde el tercio medio del brazo y con articulación de codo conservada. El segundo, es la secuela de una amputación infracondílea de extremidad inferior por osteosarcoma que presenta fístulas cutáneas con drenaje supurativo por osteomielitis en el muñón tibial. En ambos pacientes realizamos cobertura con colgajo anterolateral de muslo anastomosado a la arteria radial en el caso de la extremidad superior, y a la arteria genicular descendente en la extremidad inferior. En los dos casos el postoperatorio transcurrió sin complicaciones, logrando preservar las articulaciones del codo y de la rodilla respectivamente, así como la posterior rehabilitación protésica. Consideramos que el colgajo anterolateral del muslo permite aportar tejido de buena calidad como cobertura del muñón de amputación. Dadas las características del tejido aportado y su volumen, es idóneo tanto para cobertura de defectos agudos como de déficits de almohadillado en casos crónicos.

  5. Elaboración y validación de un cuestionario reducido de la versión española del cuestionario de calidad de vida específico para la enfermedad inflamatoria intestinal

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    Alcalá Escriche, María José

    2003-01-01

    Los cuestionarios de medida de calidad de vida en la enfermedad inflamatoria intestinal (EII), están constituidos habitualmente por un gran número de preguntas precisando tiempo para su cumplimentación y posterior análisis, lo que dificulta su utilización en la práctica clínica. El objetivo del trabajo de investigación es la elaboración y posterior validación, de un cuestionario reducido de la versión española del ¨Inflammatory Bowel Disease Questionnaire¨, Cuestionario de Calidad de Vida en ...

  6. Enfermedad inflamatoria crónica intestinal canina: hallazgos endoscópicos, bioquímicos y anatomopatológicos del tracto gastrointestinal anterior

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    R Crespo

    2015-01-01

    Full Text Available Se ha realizado un estudio retrospectivo a 26 perros, machos y hembras, que acudieron al Servicio de Medicina Interna (Unidad de Endoscopia del Hospital Clínico Veterinario de la Facultad de Veterinaria de la Universidad de Murcia (España, a los que se les diagnosticó enfermedad inflamatoria crónica intestinal del tracto gastrointestinal anterior. Los casos se eligieron en función del diagnóstico clínico, endoscópico y anatomopatológico realizado, con el objetivo de evaluar potenciales correlaciones entre estos parámetros. La disminución de proteínas totales y la albúmina no fue estadísticamente significativa en relación con el grado de inflamación de la mucosa gastrointestinal, no obstante, los animales que presentaban un infiltrado de tipo linfoplasmocitario tenían tendencia a la pérdida de albúmina. Se encontró una correlación estadísticamente significativa (P < 0,05 entre el tipo de infiltrado y la presencia de congestión en la mucosa de estómago y duodeno. Una mayor congestión a estos niveles se corresponde con el infiltrado linfoplasmocitario. También se obtuvo un resultado que, a pesar de no ser estadísticamente significativo, sí se aproxima a la significación estadística (P < 0,1 entre el grado de congestión y la intensidad del infiltrado, ya que un grado de congestión mayor de la mucosa se corresponde con una mayor intensidad de infiltrado linfoplasmocitario, tanto a nivel gástrico como duodenal. Además se vio que la friabilidad está asociada a inflamación en duodeno, y los animales que no tenían un correcto cierre del cardias presentaban esofagitis por reflujo.

  7. Valor pronóstico de la expresión de las metaloproteinasas y de sus inhibidores tisulares en células inflamatorias del estroma peritumoral estudiada mediante micromatices tisulares en piezas de prostatectomía radical

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    Rodríguez Corcos, Ángel

    2015-01-01

    Introducción: Hay nuevas evidencias que la inflamación prostática puede contribuir al carcinoma de próstata. La gran mayoría de las muestras quirúrgicas de tejido prostático muestran alguna evidencia de inflamación prostática. La identificación de un fenotipo de células mononucleares inflamatorias asociadas con el riesgo de cáncer de próstata y/o comportamiento agresivo del tumor puede ser de un gran interés, nos permitiría identificar a hombres en riesgo de desarrollar cáncer de próstata que...

  8. MODELADO MATEMÁTICO DEL COMPORTAMIENTO MECÁNICO DE UN FRAGMENTO DE CARTÍLAGO ARTICULAR

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    NANCY STELLA LANDÍNEZ

    2009-01-01

    Full Text Available El cartílago articular es un material biológico poroelástico que permite la distribución de las cargas mecánicas y el movimiento de las articulaciones. Como material bifásico, en presencia de carga, el cartílago articular deforma su matriz sólida y modifica la presión hidrostática del fluido en su interior. El objetivo del presente trabajo es implementar numéricamente un modelo matemático que predice el comportamiento mecánico del cartílago articular teniendo en cuenta la dualidad entre la matriz sólida y el líquido articular, y la característica de poroelasticidad. Utilizando una metodología basada en el método de los elementos finitos, se simuló el comportamiento de un fragmento de cartílago articular en una y dos dimensiones, ante cargas mecánicas de tensión, compresión y oscilación. El análisis de los resultados permite validar cualitativamente el comportamiento poroelástico del modelo debido a la deformación de la matriz sólida y a la salida de fluido que modifica la presión del mismo de manera similar a lo reportado en trabajos experimentales. Se concluye que el modelo matemático permite realizar predicciones sobre el comportamiento biomecánico del cartílago articular, contribuyendo al proceso investigativo en áreas como la biomecánica y la ingeniería de tejidos.

  9. Tratamiento kinésico de la ruptura total del tendón del músculo supraespinoso con y sin intervención quirúrgica

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    Aldana, Emauel

    2015-01-01

    La ruptura total del tendón del músculo del supraespinoso es una lesión que ocurre por la existencia anterior de un proceso de naturaleza traumática, inflamatoria o degenerativa, quedando una zona vulnerable y dolorosa, presentando limitaciones articulares y debilidad muscular. Esta patología afecta con mayor frecuencia a personas de edades avanzadas. Objetivo general: Evaluar la evolución de los pacientes que sufrieron rupturas totales tendinosas del músculo supraespinoso con ...

  10. Valoración isocinética de los músculos rotadores del complejo articular del hombro en jugadores de balonmano playa

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    Zapardiel Cortés, Juan Carlos

    2015-01-01

    En el presente estudio se ha realizado una valoración de la fuerza de los músculos rotadores del complejo articular del hombro mediante dinamometría isocinética. La valoración se llevó a cabo en tres grupos: un grupo de mujeres que no practican deportes de lanzamiento o remate por encima de la cabeza, un grupo de hombres en la misma situación que el grupo anterior, y un grupo de jugadores de balonmano playa. Para que la investigación se realizase de forma fiable, válida y objetiva se utilizó ...

  11. Response of first attack of inflammatory bowel disease requiring hospital admission to steroid therapy Respuesta al tratamiento esteroideo del primer brote de enfermedad inflamatoria intestinal que requiere ingreso hospitalario

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    M. Abu-Suboh Abadía

    2004-08-01

    buena en caso de remisión completa o mala si la remisión es parcial o ausente. Se realizó estudio univariado y multivariado según estadística no paramétrica de diferentes variables sociodemográficas y biológicas. Resultados: se han incluido 45 pacientes con EC y 41 con CU. La tasa de buena respuesta fue del 64,4% en la EC y del 60,9% en la CU. El análisis univariado demostró que los pacientes con buena respuesta tienen un tiempo de evolución más corto y un menor número de brotes previos (p < 0,05 para la EC. Sin embargo, el análisis multivariado no demostró que alguna variable de las analizadas tuviera valor predictivo. Conclusión: la respuesta de los brotes graves de enfermedad inflamatoria intestinal a los corticoides es alrededor del 60% en la EC y CU. Los datos del presente estudio no permiten predecir qué pacientes son los que responderán al tratamiento.

  12. Osteoporosis y enfermedad inflamatoria intestinal Osteoporosis and inflammatory bowel disease

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    L. Menchén

    2005-02-01

    Full Text Available La enfermedad inflamatoria intestinal es una entidad crónica de etiología desconocida en cuyo desarrollo influyen múltiples variables, como son la susceptibilidad individual, genética e inmunológica, así como diferentes factores ambientales. Sus manifestaciones clínicas son muy variadas y pueden afectar a otros órganos diferentes del tracto digestivo, convirtiéndose por tanto en una enfermedad multisistémica. En los últimos años existe un interés creciente por una de estas manifestaciones, la osteoporosis y la osteopenia, que puede afectar hasta al 42% de los pacientes y condiciona un importante aumento de la morbilidad. La inactividad, el tratamiento corticoideo prolongado, las deficiencias nutricionales y la propia enfermedad pueden favorecer el desarrollo de esta complicación. En esta revisión se repasan aspectos clínicos y etiológicos de la osteoporosis asociada a la enfermedad inflamatoria intestinal y se ofrecen pautas para su diagnóstico y tratamiento.Inflammatory bowel disease is a chronic disease with an unknown ethiology although multiple factors intervene such as individual, genetic and immunologic susceptibility, as well as different environmental factors. Like other multisystemic diseases, its clinical manifestations are diverse and it may affect other organs besides the gastrointestinal tract. In the last few years there is a growing interest for one of these extraintestinal manifestations, osteoporosis and osteopenia that may affect up to 42% of patients and can condition an important increase in morbility. Inactivity, prolonged corticosteroid treatment, nutritional deficiencies and the disease per se have an important role in the development of this complication. This article reviews clinical and ethiological aspects of inflammatory bowel disease associated osteoporosis and offers a strategy for diagnosis and treatment.

  13. Polirradiculopatía inflamatoria crónica sensitiva

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    Paz Zuberbuhler

    2013-06-01

    Full Text Available La polirradiculopatía inflamatoria crónica sensitiva es una entidad definida, frecuentemente subdiagnosticada y potencialmente tratable. Debe ser sospechada en pacientes con ataxia sensitiva, estudios de conducción nerviosa normales y una resonancia magnética que muestre engrosamiento y realce con gadolinio de las raíces lumbosacras. Presentamos el caso de un hombre de 57 años de edad con marcada ataxia sensitiva en pierna izquierda. Al examen físico presentaba fuerza conservada, reflejos osteotendinosos disminuidos, tacto fino y superficial reducidos por debajo de las rodillas; abatiestesia y apalestesia en ambos pies. Los estudios de conducción nerviosa eran normales, los potenciales evocados somatosensitivos tibiales con ausencia de respuesta bilateral. El líquido cefalorraquídeo presentaba hiperproteinorraquia sin células. La resonancia magnética mostró engrosamiento y realce con gadolinio de las raíces lumbosacras. El paciente fue tratado con inmunoglobulina endovenosa (IgEV a 2 g/kg durante 5 días, con buena respuesta. La evolución clínica, la hiperproteinorraquia, el realce de raíces en la resonancia magnética, la buena respuesta a la inmunoterapia y la exclusión de otras causas de ataxia sensitiva fueron compatibles con el diagnóstico de polirradiculopatía inflamatoria crónica sensitiva. Para el diagnóstico de esta enfermedad se requiere la identificación del compromiso aislado de las raíces sensitivas.

  14. Respuesta inflamatoria de la Célula Acinar a diferentes lípidos generados en grasa necrotica durante la pancreatitit aguda

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    Mateu Rico, Alberto

    2016-01-01

    [ES] La pancreatitis aguda (PA) es una enfermedad inflamatoria del páncreas en la que tiene lugar la secreción anormal al intersticio pancreático de enzimas digestivas como lipasas y fosfolipasas. Durante la PA se liberan grandes cantidades de ácidos grasos (AGs) y fosfolípidos (FLs) desde grasa peripancreática, habiéndose demostrado su capacidad para modular la respuesta inflamatoria. Como resultado del daño inicial del páncreas, se produce un reclutamiento masivo de leucocitos hacia la glán...

  15. Tratamiento del paciente con artrosis

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    Francisco Vargas Negrín

    2014-01-01

    Full Text Available El manejo terapéutico del paciente con artrosis tiene como objetivo disminuir la sintomatología dolorosa e inflamatoria, mejorar la capacidad funcional del paciente y la aplicación de intervenciones terapéuticas eficaces y lo más seguras posibles. Un enfoque centrado en el paciente implica su participación activa en el diseño del plan terapéutico y en la toma de decisiones informadas oportunas en todas las etapas de la enfermedad. La educación terapéutica, la actividad física y el ejercicio terapéutico junto con el control de peso, en caso de sobrepeso u obesidad, constituyen el núcleo central del tratamiento. Los autocuidados individuales y por los familiares son fundamentales en el control del día a día del paciente. El uso de terapias físicas, ayudas técnicas (bastón, etc. y de fármacos tipo analgésicos simples, opioides y antiinflamatorios tiene evidencias demostradas en el control del dolor, mejora la funcionalidad y la calidad de vida del paciente y una clara recomendación de uso en el tratamiento de la artrosis. La cirugía conservadora y la de reemplazo articular se indican en los casos en los que no se logran los objetivos terapéuticos en casos concretos.

  16. Hiperplasia Fibrosa Inflamatoria: reporte de un caso Inflammatory Fibrous Hyperplasia: case report

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    A Casian Romero; P Trejo Quiroz; C De León Torres; D Carmona Ruiz

    2011-01-01

    Introducción: La Hiperplasia Fibrosa Inflamatoria es una alteración caracterizada por un agrandamiento del tejido conectivo; se considera una lesión reactiva ya que se presenta como respuesta a una irritante crónico local; es una lesión común en pacientes adolescentes y de edad adulta debido a que su etiología principal se asocia a maloclusión, al uso de aparatología protésica u ortodóntica mal ajustada, así como a la presencia de biofilm. Sin embargo, la población infantil, principalmente en...

  17. Hipnosis clínica en un caso de enfermedad inflamatoria intestinal

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    J. C. FERNÁNDEZ MÉNDEZ

    2000-01-01

    Full Text Available Un sujeto de veinte años con una enfermedad inflamatoria intestinal fue tratado con hipnosis. Hasta donde sabemos es el primer informe que documenta el empleo de la hipnosis clínica en un paciente con enfermedad de Crohn, para reducir el estrés y afrontar las demandas originadas por la patología digestiva. Además, otro aspecto importante en el presente trabajo, es el registro exhaustivo y consecutivo de la sintomatología gastrointestinal, un punto débil en la gran mayoría de los trabajos precedentes. Al finalizar la intervención psicológica disminuyó notablemente el nivel de intensidad del estrés, el número de deposiciones diarias así como la intensidad del dolor abdominal. Estos resultados se mantuvieron a lo largo del seguimiento.

  18. Traducción, adaptación y validación al español del cuestionario de calidad de vida de 32 ítems (IBDQ-32 de la enfermedad inflamatoria intestinal Spanish translation, adaptation, and validation of the 32-item questionnaire on quality of life for inflammatory bowel disease (IBDQ-32

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    M. Masachs

    2007-09-01

    Full Text Available Introducción: la medida de la calidad de vida relacionada con la salud (CVRS tiene una reconocida importancia en la evaluación, el manejo y el seguimiento de la enfermedad inflamatoria intestinal. El instrumento de medida más utilizado es la versión de 32 ítems del Inflammatory Bowel Disease Questionnaire (IBDQ-32, que no está adaptado al español. Objetivo: traducir la versión del IBDQ-32 al español y determinar su validez, fiabilidad y sensibilidad tanto en la colitis ulcerosa como en la enfermedad de Crohn. Método: estudio prospectivo en dos fases: traducción y posterior validación del IBDQ-32 al español. La traducción se ha basado en la versión al español validada del IBDQ-36, y los ítems del IBDQ-32 no incluidos en el IBDQ-36 se tradujeron "de novo". Una vez terminada la traducción del IBDQ32, se aplicó un cuestionario específico de comprensión. Para determinar las propiedades psicométricas del IBDQ-32, un grupo de pacientes completó el IBDQ-36 ya validado al español y el IBDQ-32. Resultados: se han incluido 84 pacientes (53 con enfermedad de Crohn y 31 con colitis ulcerosa. La mediana de la puntuación global de ambos cuestionarios en los 84 pacientes no fue diferente (6,1 vs. 6,2, p = ns y su correlación de Spearman fue muy significativa (r = 0,97, p Introduction: the measurement of health-related quality of life (HRQoL has an established relevance in the assessment, management, and follow-up of inflammatory bowel disease. The most commonly used measuring instrument is the 32-item version of Inflammatory Bowel Disease Questionnaire (IBDQ-32, which has never been adapted to Spanish. Objective: to translate IBDQ-32 into Spanish, and to establish its validity, reliability, and sensitivity both in ulcerative colitis and Crohn's disease. Method: a prospective study in two phases -translation into Spanish and subsequent validation of IBDQ-32. Translation was based on the validated Spanish version of IBDQ-36, and IBDQ-32

  19. Neuritis óptica inflamatoria Inflammatory optic neuritis

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    T. Ayuso

    2009-08-01

    Full Text Available La neuritis óptica inflamatoria (NO es la causa más frecuente de pérdida visual aguda en adultos jóvenes. Aunque el pronóstico visual es excelente en la mayoría de los casos, muchos pacientes desarrollarán otra patología como esclerosis múltiple en la evolución posterior. La historia natural de la NO ha sido estudiada en múltiples trabajos en los últimos años; uno de los más importantes es el Optic Neuritis Treatment Trial. La Resonancia Magnética tiene un papel fundamental en el diagnóstico etiológico de la NO y en la predicción del riesgo de conversión a esclerosis múltiple. Recientemente se han incorporado nuevas técnicas exploratorias como la tomografia de coherencia óptica, útil para el diagnóstico y pronóstico; se han identificado biomarcadores séricos que ayudan en el diagnóstico de otras patologías de naturaleza autoinmune que producen NO. Un mejor conocimiento de los datos clínicos y exploratorios de la NO típica permitirá un estudio diagnóstico más rápido y certero. El tratamiento de la NO con esteroides debe ser individualizado teniendo en cuenta que no modifican el pronóstico a largo plazo y en pacientes con alto riesgo de conversión a esclerosis múltiple debe plantearse terapia inmunomoduladora. Este trabajo revisa los datos existentes en la literatura referentes a las manifestaciones clínicas, el diagnóstico etiológico y diferencial y tratamiento de la NO inflamatoria.Inflammatory Optic Neuritis (ON is the most frequent cause of acute visual loss in young adults. Although the visual prognosis is excellent in the majority of cases, many patients develop pathology, such as multiple sclerosis, in its subsequent evolution. The natural history of ON has been studied in numerous works in recent years; one of the most important of which is Optic Neuritis Treatment Trial. Magnetic Resonance plays a fundamental role in the etiological diagnosis of ON and in predicting the risk of conversion into

  20. Desarrollo de un nuevo medicamento con acetato de hidrocortisona para el tratamiento de la enfermedad inflamatoria intestinal (EII)

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    Ascaso Anglés, Magda

    2015-01-01

    El objetivo principal de este estudio consiste en la investigación y desarrollo de la formulación de un nuevo medicamento semisólido con acetato de hidrocortisona en forma farmacéutica de enema rectal. Esta formulación pretende cubrir un vacío terapéutico en el tratamiento de la Enfermedad inflamatoria intestinal, en concreto la colitis ulcerosa. Para ello es necesario tener en cuenta las especificaciones indicadas en la modificación de la Ley del Medicamento y posteriores circulares del Mini...

  1. Amiloidosis secundaria en la enfermedad inflamatoria intestinal Secondary amyloidosis in Chrohn's disease

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    S. Seijo Ríos

    2008-12-01

    Full Text Available La amiloidosis es una entidad clínica que se produce a consecuencia del depósito a nivel extracelular de un material proteico amorfo, causando una desorganización de la arquitectura normal de múltiples órganos y tejidos y, por tanto, una alteración funcional de los mismos. La amiloidosis secundaria es una complicación infrecuente pero muy grave que aparece en el contexto de neoplasias, enfermedades infecciosas e inflamatorias de curso crónico, como es el caso de la enfermedad inflamatoria intestinal, principalmente enfermedad de Crohn, ensombreciendo el pronóstico de estos pacientes. A continuación presentamos dos casos clínicos correspondientes a dos pacientes con enfermedad de Crohn que desarrollaron amiloidosis secundaria.Amyloidosis is a clinical entity that results from the deposition of an extracellular protein material that causes disruption in the normal architecture of multiple organs and tissues, and impairs their function. Secondary amyloidosis is a rare but serious complication that may worsen the prognosis of patients with cancer, infection or chronic inflammatory disease, including inflammatory bowel disease, particularly Crohn's disease. We report two cases of Crohn's disease associated with secondary amyloidosis.

  2. Manifestaciones reumatológicas de la enfermedad inflamatoria intestinal Rheumatologic manifestations of inflammatory bowel disease

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    Octavio Germán Muñoz Maya

    2006-01-01

    Full Text Available La enfermedad inflamatoria intestinal (EII se caracteriza por la activación inapropiada del sistema inmune de la mucosa intestinal y sus dos formas de presentación son: la colitis ulcerativa y la enfermedad de Crohn. Las manifestaciones extraintestinales se presentan hasta en el 36% de los pacientes y pueden comprometer cualquier órgano o sistema. La disfunción inmune se caracteriza por el desequilibrio entre los mediadores proinflamatorios y los antinflamatorios y se expresa como una enfermedad sistémica. Las manifestaciones reumatológicas asociadas a la EII son de tres tipos: la artritis periférica, la espondiloartropatía y una tercera categoría que incluye lesiones dérmicas, oftálmicas y del metabolismo óseo, entre otras. El manejo de estas manifestaciones se basa en la terapia sistémica para el control de la actividad inflamatoria local utilizando esteroides, derivados de la 5-ASA, inmunomoduladores y, en los últimos años, terapia anti-TNF. The main feature of inflammatory bowel disease (IBD is the continuous activation of the mucosa-associated immune system; the disease has two major forms of presentation: ulcerative colitis and Crohn´s disease. The extraintestinal manifestations are present in 36% of patients, and any organ can be affected. There is an imbalance between proinflammatory and antinflammatory cytokines leading to a systemic disease. The rheumatologic manifestations of the IBD are: Peripheral arthritis, spondyloarthropathy and a third category that includes dermic and ocular lesions as well as metabolic bone disease. Control of the extraintestinal manifestations is based on systemic therapy with steroids, 5-ASA derivatives and biological anti-TNF therapy.

  3. Estudio Transversal sobre la Relación entre Neuroticismo y Curso Clínico en Pacientes con Enfermedades Inflamatorias Intestinales

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    Juan Carlos Fernández Méndez

    2010-01-01

    Full Text Available En el presente trabajo se analiza la relación que existe entre neuroticismo y diversas condiciones médicas que hacen referencia al curso clínico de las enfermedades inflamatorias intestinales. Para desarrollar la investigación se tomaron medidas en neuroticismo mediante el Inventario de Personalidad de Eysenck a 106 participantes diagnosticados de colitis ulcerosa y enfermedad de Crohn. Los resultados reflejaron que la muestra global de pacientes con enfermedades inflamatorias intestinales mostraba valores en neuroticismo que se hallaban dentro de la normalidad; sin embargo, se pudo verificar que los valores diferían dependiendo del curso clínico de la patología. El estudio sugiere que las variaciones en neuroticismo son considerables cuando se analizan los datos de la muestra general y cuando se tienen en cuenta diversas condiciones del curso clínico.

  4. Proyecciones radiológicas de muñeca para visualizar adecuadamente la superficie articular del radio en fracturas de radio distal “de acuerdo a ángulos hallados mediante modelos virtuales 3D”

    OpenAIRE

    Penagos López, Rodrigo Alberto

    2012-01-01

    La fractura del radio distal es una patología frecuente que muchas veces requiere tratamiento quirúrgico. Este puede realizarse con placas y tornillos, en donde es importante visualizar la adecuada posición del material de osteosíntesis. Se necesita tener unas proyecciones radiológicas que permitan ver adecuadamente la superficie articular sin interposición de estructuras para tener plena seguridad de la posición de los tornillos extraarticulares, para lo cual se propone una nueva proye...

  5. Efficacy of intravenous iron in treating iron deficiency anaemia in patients with inflammatory bowel disease: Are there predictors of response? Eficacia del hierro intravenoso en el tratamiento de la anemia ferropénica en pacientes con enfermedad inflamatoria intestinal: ¿Existen factores predictivos de respuesta?

    Directory of Open Access Journals (Sweden)

    Rocío Ferreiro Iglesias

    2011-05-01

    Full Text Available Introduction: in inflammatory bowel disease (IBD iron deficiency anaemia (IDA is a very common disorder. Until recently, oral iron has been the mainstay therapy, nevertheless it has been associated with intolerance and noncompliance. Therefore, the goal of our study was to evaluate the efficacy of intravenous iron in IDA in IBD patients and the secondary aim was to investigate whether other potential factors could influence in the response to the treatment. Design: an open-label, prospective, consecutive, single centre study. Material and methods: we performed our study in patients with ulcerative colitis (UC or Crohn's disease (CD with severe anaemia or intolerance with oral iron. All of them received intravenous sacarose iron and did biochemistry profile with haemoglobin (Hb. Moreover, the correlation with other variables was studied: age, sex, smoking habit, IBD type, previous surgery and type of surgery and other treatments. Response was defined as Hb increase of ≥ 2 g/dL or normalization of the levels. Results: fifty-four patients were included into the study, 34 (63% with UC y 20 (37% with CD, 18 (33.3% men and 36 women (66.6% and the average was 48 ± 14 years. The total proportion of responders was 52% (SD ± 05; 43% of the patients reached Hb ≥ 2 g/dl and y 9% of them normalized Hb. Only the utilization of 5-ASA was associated with low response to iron treatment (p Introducción: la anemia por déficit de hierro es un problema frecuente en la enfermedad inflamatoria intestinal (EII. Un número no despreciable de pacientes no responde o presenta intolerancia al hierro oral. El objetivo de nuestro estudio es evaluar la eficacia del hierro sacarosa intravenoso (Venofer® en los pacientes con EII así como los potenciales factores que pueden influir en la respuesta al mismo. Diseño: estudio abierto, unicéntrico y con una inclusión consecutiva de casos. Material y métodos: se incluyeron pacientes con colitis ulcerosa (CU y enfermedad

  6. Recomendaciones de vacunación en pacientes con enfermedad inflamatoria intestinal (EII

    Directory of Open Access Journals (Sweden)

    C. Rodríguez

    2013-04-01

    Full Text Available El concepto de enfermedad inflamatoria intestinal (EII engloba tres entidades: la colitis ulcerosa (CU, la enfermedad de Crohn (EC y la colitis inclasificable (CI. Estas enfermedades tienen en común el curso crónico y recidivante, alternando épocas de marcada actividad inflamatoria con otras quiescentes, en las que el paciente permanece asintomático. Durante muchos años la base del tratamiento, sobre todo en las fases agudas, se basó en el uso de corticoides. Sin embargo, a lo largo de las últimas décadas hemos asistido a avances importantes desde el punto de vista terapéutico. Así se estima que, a lo largo de la evolución de la enfermedad, el 80% de los pacientes van a precisar corticoides, el 40% inmunomoduladores (IMM y hasta un 20% necesitará un fármaco biológico para el control de su enfermedad. Si bien todo ello se acompaña de una mejora en la calidad de vida, disminuyendo la necesidad de ingresos e intervenciones quirúrgicas, su uso implica también un incremento en el riesgo de sufrir infecciones, bien por gérmenes habituales en la comunidad o por gérmenes oportunistas. Las infecciones, además, son causa de morbimortalidad asociada a EII y algunas de ellas son prevenibles con vacunas, de ahí la importancia que los programas de vacunación están adquiriendo en este grupo de pacientes. Presentamos una revisión de la literatura al respecto y proponemos unas recomendaciones de vacunación para los pacientes diagnosticados de EII.

  7. Nutrición y enfermedad inflamatoria intestinal Nutrition and inflammatory bowel disease

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    E. Cabré Gelada

    2007-05-01

    Full Text Available En este artículo se revisan las causas y consecuencias de los déficits nutricionales en la enfermedad inflamatoria intestinal, con especial énfasis en sus implicaciones fisiopatológicas. Además, se sientan las bases de la intervención nutricional en estos pacientes tanto desde la perspectiva del consejo dietético como del uso de soporte nutricional especializado. Se hace un especial hincapié en la discusión de las evidencias existentes en favor de un papel terapéutico primario de la nutrición artificial y algunos nutrientes específicos en la enfermedad de Crohn.In this paper, the causes and consequences of nutritional deficiencies in inflammatory bowel disease are reviewed. Particular emphasis is made on their pathophysiological implications. In addition, the basis for nutritional intervention in these patients are described (both in terms of dietary counseling and the use of specialized nutritional support. Particular mention is made on the current evidences in favour of a primary therapeutic role of artificial nutrition and some specific nutrients in Crohn's disease.

  8. Pelvic inflammatory disease Enfermedad inflamatoria pélvica

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    Regla Fang Mederos

    2007-02-01

    Full Text Available

    A bibliographical revision on the main topics referred to the acute pelvic inflammatory disease which includes definition, risk factors, diagnosis, and treatment with the objective to create a supporting educational aid to the teaching of students of 4th and 6th year of the medicine and nursing specialty in their rounds on the services of gynecology and obstetrics.

    Revisión bibliográfica sobre los principales temas referidos a la enfermedad inflamatoria pélvica aguda que incluye concepto, factores de riesgo, cuadro clínico, diagnóstico y tratamiento, con el objetivo de crear un material de apoyo a la docencia de estudiantes de cuarto y sexto año de la especialidad de medicina y de enfermería en su rotación por los Servicios de Ginecología y Obstetricia.

  9. Hiperplasia Fibrosa Inflamatoria: reporte de un caso Inflammatory Fibrous Hyperplasia: case report

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    A Casian Romero

    2011-08-01

    Full Text Available Introducción: La Hiperplasia Fibrosa Inflamatoria es una alteración caracterizada por un agrandamiento del tejido conectivo; se considera una lesión reactiva ya que se presenta como respuesta a una irritante crónico local; es una lesión común en pacientes adolescentes y de edad adulta debido a que su etiología principal se asocia a maloclusión, al uso de aparatología protésica u ortodóntica mal ajustada, así como a la presencia de biofilm. Sin embargo, la población infantil, principalmente en dentición mixta, no está exenta de presentar esta alteración, aunque en menor proporción. Objetivo: Presentar el caso clínico de un paciente en dentición mixta que presenta una Hiperplasia Fibrosa Inflamatoria con el fin de presentar las características clínicas e histopatológicas de la lesión, enfatizando la importancia de un temprano y correcto diagnóstico. Presentación del Caso: Paciente masculino de 8 años de edad que se presenta a la clínica por presentar lesiones cariosas y malposición dental. A la exploración se observa un agrandamiento gingival localizado, en el órgano dentario 41. Se decide tomar una biopsia de la lesión para establecer el diagnóstico. Conclusión: Debido a que existen varias lesiones con características clínicas muy similares a las que presentaba el paciente, es necesario conocer los diagnósticos diferenciales, así como realizar el diagnóstico temprano de la lesión para poder llevar a cabo un tratamiento eficaz y adecuado.Introduction: The Inflammatory Fibrous Hyperplasia (IFH is a disorder consisting in a connective tissue overgrowth; it is considered a reactive lesion because it appears in response to a chronic and local irritant; this is a very common oral lesion in adolescents and adults, its etiology is associated with dental malocclusion, use of over-extended prosthetic and orthodontic appliances, and with the presence of biofilm (plaque. Nevertheless, child population, mainly patients

  10. Tratamiento nutricional en la enfermedad inflamatoria intestinal Nutritional management of inflammatory bowel disease

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    C. Pérez Tárrago

    2008-10-01

    Full Text Available Los pacientes con enfermedad inflamatoria intestinal presentan mayor riesgo de malnutrición. Por este motivo es muy frecuente que se precise un adecuado soporte nutricional. En estos pacientes se debe utilizar la nutrición enteral a menos que existan contraindicaciones. El soporte nutricional como tratamiento primario no está indicado en adultos (al haberse demostrado una mayor eficacia del tratamiento esteroideo salvo en caso de intolerancia o falta de respuesta al tratamiento médico. Por el contrario, la nutrición enteral se considera el tratamiento de primera línea en niños. No existe un claro beneficio con el uso de fórmulas específicas (grasa modificada, glutamina... por lo que no se aconseja su uso rutinario. A pesar de los grandes avances técnicos y científicos existen aún numerosos campos en los que ampliar conocimientos; algunos de los mismos se esbozan en la presente publicación.Patients with inflammatory bowel disease present higher risk for hyponutrition. For this reason, an adequate nutritional support is frequently needed. In these patients, enteral nutrition should be used unless there exist contraindications. Nutritional support as the primary therapy is no indicated in adults (since steroidal therapy has shown to be more effective but in the case of intolerance or lack of response to medical treatment. By contrast, enteral nutrition is considered a first line therapy in children. There is no clear benefit with the use of specific formulas (modified fat, glutamine..., so that their routine use is not recommended. In spite of the great technical and scientific advances, there are still many fields in which knowledge should be broaden; some of them are pointed out in this publication.

  11. [Intra-articular injections].

    Science.gov (United States)

    Chapelle, Ch

    2015-09-01

    It is not unusual for a specialist or general practitioner to be presented with a pathology which necessitates the use of an intra-articular injection of corticosteroids, hyaluronic acid or a local anaesthetic. It would seem to be interesting to update and to precise the techniques and methods of intraarticular injections which have appeared in recent international publications, when we know that 30 % of the injections given into the knee and so called "dry" are incorrect and, therefore, inefficient. The indication of an articular injection depends, firstly, on the diagnosis which should be done with great care; after which should be an objective analysis complete with secondary effects linked to both the injection and the product used. The conditions of asepsis, the choice of needles and quantities of the injection and even the ways of the injections should be reviewed in detail. The last studies clearly question the secondary effects of the cartilage degradations of the cortisone given as an intra-articular injection and shows its efficiency on the pain and inflammatory phenomonen in osteoarthritis. Studies on hyaluronic acid are often contradictory going from a modest result to an important pain relief but it is necessary to be aware that the objective criteria are difficult to interpret. The use of local anaesthetics in intra-articular is limited by the few indications in view of the major risk of aggravating the pre-existing lesions by the disappearing signs of pain.

  12. Articular cartilage stem cell signalling

    OpenAIRE

    Karlsson, Camilla; Lindahl, Anders

    2009-01-01

    The view of articular cartilage as a non-regeneration organ has been challenged in recent years. The articular cartilage consists of distinct zones with different cellular and molecular phenotypes, and the superficial zone has been hypothesized to harbour stem cells. Furthermore, the articular cartilage demonstrates a distinct pattern regarding stem cell markers (that is, Notch-1, Stro-1, and vascular cell adhesion molecule-1). These results, in combination with the positive identification of...

  13. Citocinas anti-inflamatorias y sus acciones y efectos en la sepsis y el choque séptico (Anti-inflammatory cytokines and their actions and effects in the sepsis and septic shock.

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    González Álvarez, Ricardo

    2009-08-01

    Full Text Available ResumenLas citocinas anti- inflamatorias reconocidas son las interleucinas (IL IL-4, IL-10, IL-6, IL-13, el factor estimulante de colonia granulocito –macrófago (FEC-GM y el interferón alfa (IFN-alfa, los cuales tienen la capacidad de inhibir la liberación de las citocinas pro-inflamatorias y de inducir la producción del antagonista del receptor de la IL-1 y la liberación del receptor soluble del FNT, los cuales limitan algunas de las actividades de las citocinas pro-inflamatorias IL-1 y FNT-α. Sin embargo, los eventos que ocurren durante la inflamación y la sepsis no son tan simples como parajustificar siempre acciones antagonistas entre las citocinas proinflamatorias y las anti-inflamatorias, las cuales, pueden tener acorde con las circunstancias un comportamiento dual y/o antagónico en el choque séptico. Así por ejemplo la IL-10 tiene propiedades anti-inflamatorias, lo cual ocurre en la diabetes auto-inmune cuyo comienzo y desarrollo son acelerados en ratones transgénicos que sobre-expresan la IL-10 en los islotes pancreáticos. Esto también ocurre con la IL-10 en un modelo de uveítis en el que se incrementó la inflamación ocular. Ejemplos como los anteriores son extensivos a otras citocinas anti-inflamatorias como la IL-4, IL-13, el factor estimulante de colonia granulocito –macrófago y el factor beta transformante del crecimiento (FβTC, y que serán mencionadas y descritas en el presente artículo de revisión, así como los factores que determinan ese comportamiento dual de las citocinas.Summaryinterleucinas. The anti-inflammatory cytokines which are recognized are the interleukins (IL IL-4, IL-10, IL-13, interferon-alpha (IFN-α and because they are able to inhibit the release of pro inflammatory cytokines, to induce the production of IL-1 receptor antagonist (IL-ra and the release of soluble TNF receptor and to limit some of the pro-inflammatory activities of IL-1 and TNF. However the events which occur during

  14. Nuevos mecanismos protectores en la enfermedad inflamatoria intestinal sistema endocannabinoide y haptoglobina /

    OpenAIRE

    Márquez Mosquera, Lucía

    2012-01-01

    La Enfermedad Inflamatoria Intestinal (EII) (enfermedad de Crohn (EC) y Colitis Ulcerosa (CU)), es una enfermedad crónica, poligénica y compleja, de curso recurrente, que afecta al tracto gastrointestinal. Estudios recientes in vitro y modelos experimentales en animales sugieren que el Sistema Endocannabinoide (SE) y la Haptoglobina (Hp) pueden tener un papel protector en la EII. Estudio 1: Sistema Endocannabinoide y EII Se analizó, mediante Western-Blot e inmunohistoquímica, la pre...

  15. Donador exógeno de óxido nítrico en la respuesta inflamatoria hepática y hemodinámica después de choque hemorrágico

    OpenAIRE

    Roberto Anaya-Prado; Luis H. Toledo-Pereyra; Ren Feng Guo; Jayne Reuben; Ward, Peter A.

    2008-01-01

    Introducción: El choque hemorrágico resulta en estrés oxidativo celular e inducción de respuesta inflamatoria. Investigamos la capacidad del nitroprusiato de sodio para reducir la lesión tisular en un modelo animal de choque hemorrágico no controlado. Material y métodos: 72 ratas Sprague-Dawley distribuidas en cuatro grupos: sham/salina, sham/nitroprusiato de sodio, choque/ salina, choque/nitroprusiato de sodio. Se provocó choque hemorrágico (3 ml/100 g) en un periodo de 15 minutos; corte de ...

  16. Comportamiento de variables epidemiológicas en la neuropatía óptica inflamatoria infecciosa. Centro Oftalmológico de Holguín. 2009

    OpenAIRE

    Enma Estrella de la Torre-Rodríguez; María Eugenia Escobar-Pérez; Lismay Grin-Pupo; Félix Fuentes-Díaz

    2012-01-01

    Abordó un estudio descriptivo del comportamiento de algunas variables epidemiológicas en un universo de 18 pacientes con neuropatía óptica inflamatoria en el Centro Oftalmológico de Holguín de enero a diciembre de 2009. La muestra estuvo representada por 12 pacientes con etiología infecciosa. El mayor número de pacientes se incluyó en el grupo de edad de 15 a 29 años. Las ocupaciones de riesgo más frecuentes fueron los estudiantes, las amas de casa y criadores de palomas. Los perros, gatos y...

  17. Actividad inflamatoria en múltiples placas ateroscleróticas en pacientes fallecidos por infarto agudo de miocardio

    Directory of Open Access Journals (Sweden)

    Alejandro García Escudero

    2009-01-01

    Full Text Available IntroducciónEstudios clínicos y anatomopatológicos sugieren que los procesos inflamatorios tienen un papel importante en la inestabilidad de la placa aterosclerótica, dado que en pacientes con síndromes coronarios agudos se observan infiltrados inflamatorios difusos en las arterias coronarias.ObjetivosEvaluar y localizar la distribución de placas vulnerables e infiltrados inflamatorios en pacientes fallecidos por infarto agudo de miocardio.Material y métodosMediante microscopia óptica se estudiaron las arterias coronarias de 58 pacientes fallecidos por infarto de miocardio. En las arterias coronarias relacionadas con el infarto y en las no relacionadas se registraron las siguientes variables: presencia de trombo, rotura de placa, hemorragia intraplaca y presencia de infiltrado inflamatorio.ResultadosAl analizar las diferencias existentes entre las arterias responsables del infarto y en las no responsables se encontraron diferencias significativas con respecto a la presencia de trombo (69% versus 38%; p < 0,008 y de hemorragia intraplaca (69% versus 50%; p < 0,03. No se encontró una diferencia significativa entre la arteria responsable y la no responsable al evaluar la presencia de infiltrado inflamatorio en las placas ateroscleróticas (77% versus 71%; p = ns.ConclusiónEn el infarto agudo de miocardio se comprobó la presencia de actividad inflamatoria que afectaba a más de un vaso, con compromiso de otras arterias además de la responsable del infarto. Se detectó también accidente agudo de placa en más de una arteria coronaria.REV ARGENT CARDIOL 2009;77:81-87.

  18. Revisión de la casuística neurológica de naturaleza inflamatoria reportada en equinos durante los años 2005 a 2009 en el Laboratorio de Diagnóstico del Instituto Colombiano Agropecuario (ICA) / Histologic review of cases with neurological siymtoms in horses reported at the Instituto Colombiano Agropecuario (ica) Bogota during 2005 to 2009

    OpenAIRE

    Urrea Quiroga, Martha Isabel

    2012-01-01

    Como parte de la práctica de interés académico, en este trabajo se realizó una revisión y profundización histopatológica de las lesiones de cada una de las enfermedades de tipo inflamatorio del SNC, diagnosticadas en el ICA sede Bogotá, entre los años 2005 a 2009 en la especie equina. Durante el periodo de tiempo predeterminado, se remitieron al laboratorio 115 casos (cadáveres o tejidos) de equinos que presentaron, según la historia de remisión, sintomatología nerviosa; de estos casos, no se...

  19. Comparación de los efectos de dos técnicas de electroanalgesia en la enfermedad articular degenerativa tarsometatarsiana del equino Comparison between the effects of two electroanalgesical technics in the tarsometatarsal degenerative joint disease

    Directory of Open Access Journals (Sweden)

    J.A. García Liñeiro

    2006-12-01

    Full Text Available Se realizó una experiencia para comparar los resultados analgésicos entre TENS (cuyas siglas derivan de la expresión en inglés: transcutaneus electrical neuromuscular stimulation y el EMAR (estimulación mecánica por acción refleja de efectividad comprobada en medicina equina, sobre un modelo clínico de 10 equinos afectados por enfermedad articular degenerativa tarsometatarsiana con un protocolo diagnóstico estandarizado. Sobre la misma población se hicieron los dos tratamientos con intervalos de 6 meses entre uno y otro comparándose luego los resultados. Las aplicaciones en cada tratamiento fueron diarias y el control clínico se realizó post-tratamientos cada dos días (5 controles totales por los mismos tres profesionales para mantener el mismo esquema de evaluación, considerándose como parámetros: a- Trote en línea recta y en círculo, terreno blando y duro b­Flexión forzada c- Test del tarso (test de Churchill. Se realizó la prueba de Mc Nemar para comparar los grados de claudicación y respuestas a la flexión forzada, observados durante el 5to control, obtenidos luego de aplicar EMAR y TENS, y no se encontraron diferencias significativas entre ambos tratamientos (p=0,2568. También, se comparó el resultado del Test del Tarso (Test de Churchill luego de aplicar EMAR y TENS, con la prueba de Mc Nemar, no encontrándose diferencias significativas entre ambos (p= 0,7055. Se determina que hay muy poca diferencia entre ambos tratamientos fisioterápicos, observándose una disminución importante a partir del control 2. De estos resultados podemos inferir que ambos métodos pueden utilizarse con éxito en forma indistinta, seleccionándolos de acuerdo con las ventajas y desventajas de su aplicación.A clinical model was designed in order to compare the analgesic effects of TENS (transcutaneous electrical neuromuscular stimulation and RAME (reflex action mechanical electrostimulation, since both methods have been proven

  20. Detección de glicosaminoglicanos de la matriz del cartílago articular en el líquido sinovial de carpo equino con fractura en esquirla Detection of glycosaminoglycans of the articular cartilage matrix in the synovial fluid of equine carpal joint with chip fracture

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    H Adarmes

    2008-01-01

    Full Text Available El objetivo de este estudio fue determinar si la duración de la fractura en esquirla de carpo en equinos Fina Sangre de Carrera afecta la concentración sinovial de glicosaminoglicanos totales (GAGsT y de una fracción de éstos, que corresponde a los GAGs diferentes del ácido hialurónico o GAGs sulfatados (GAGsS. Se analizaron dos grupos de equinos con fractura en esquirla de distinta duración: uno con un período menor a los 30 días desde el diagnóstico hasta la artrocéntesis (grupo I (n = 14 y otro, con un período igual o mayor a los 60 días (grupo II (n = 14, y como grupo control se obtuvo líquido sinovial de articulaciones de carpo sanas al examen visual post mortem, de equinos de matadero luego del sacrificio (n = 14. La concentración de proteínas, GAGsT y GAGsS en el líquido sinovial se realizó espectrofotométricamente. La concentración de proteínas fue significativamente mayor (P The purpose of this study was to establish if the duration of chip fracture in equine carpal joint of purebred horses, affects the synovial concentration of total glycosaminoglycans (GAGsT and glycosaminoglycans that are different from hyaluronic acid or sulfated GAGs (GAGsS. Two groups of horses with different duration of fracture period were analyzed: one group (group I with a period of less than 30 days from carpal fracture diagnosis to arthrocentesis (n=14, and another group (group II with a period similar or longer than 60 days (n=14. Normal synovial fluid was collected from healthy carpal joints of crossbred horses immediately after slaughter (n=14, without any major damage at post mortem examination. The synovial fluid concentration of protein and GAGs was determined spectrophotometrically. Protein concentration showed a significant increase (P 0.05 between control group (0.19 ± 0.12 g/L and both fracture groups (0.15 ± 0.09 g/L (group I and 0.17 ± 0.17 g/L (group II. The concentration of hyaluronic acid, obtained by the difference

  1. La reacción inflamatoria en la fisiopatogenia de la obesidad

    OpenAIRE

    Ma Victoria Domínguez García; Gerardo Gabriel Huitrón Bravo; Anaí A. Mendoza López

    2012-01-01

    La obesidad se caracteriza por inflamación crónica. Entre los biomarcadores de inflamación encontramos las citocinas producidas por los adipocitos y los macrófagos: TNF - a, IL - 6, leptina, adiponectina y resistina. La relación adipocito-macrófago impacta la respuesta inflamatoria observada en la obesidad, la cual tiene una relación directa con la cantidad de macrófagos acumulados en el tejido adiposo blanco, que contribuye a la producción de mediadores de la inflamación. En este documento s...

  2. Miopatía inflamatoria con compromiso inicial de músculos respiratorios y artritis reumatoidea

    OpenAIRE

    Martín Hunter; Irene Telias; Victoria Collado; Judith Sarano; Clarisa Álvarez; Juan Pablo Suárez

    2014-01-01

    Las miopatías inflamatorias constituyen un grupo heterogéneo de enfermedades musculares adquiridas de presentación subaguda, crónica y a veces aguda. Las entidades clínicas más frecuentes son la dermatomiositis, la polimiositis, la miositis necrotizante autoinmune y la miositis por cuerpos de inclusión. Suelen presentarse con debilidad muscular con predominio proximal y simétrica, pero rara vez comprometen los músculos respiratorios. Presentamos el caso de una mujer de 39 años con miopatía in...

  3. Citocinas anti-inflamatorias y sus acciones y efectos en la sepsis y el choque séptico - Anti-inflammatory cytokines and their actions and effects in the sepsis and septic shock

    Directory of Open Access Journals (Sweden)

    González Álvarez, Ricardo

    2009-09-01

    Full Text Available ResumenLas citocinas anti- inflamatorias reconocidas son las interleucinas (ILIL-4, IL-10, IL-6, IL-13, el factor estimulante de colonia granulocito – macrófago (FEC-GM y el interferón alfa (IFN-alfa, los cuales tienen la capacidad de inhibir la liberación de las citocinas pro-inflamatorias y de inducir la producción del antagonista del receptor de la IL-1 y la liberación del receptor soluble del FNT, los cuales limitan algunas de las actividades de las citocinas pro-inflamatorias IL-1 y FNT-α. Sin embargo, los eventos que ocurren durante la inflamación y la sepsis no son tan simples como para justificar siempre acciones antagonistas entre las citocinas pro-inflamatorias y las antiinflamatorias, las cuales, pueden tener acorde con las circunstancias un comportamiento dual y/o antagónico en el choque séptico. Así por ejemplo la IL-10 tiene propiedades anti-inflamatorias, lo cual ocurre en la diabetes auto-inmune cuyo comienzo y desarrollo son acelerados en ratones transgénicos que sobre-expresan la IL-10 en los islotes pancreáticos. Esto también ocurre con la IL-10 en un modelo de uveítis en el que se incrementó la inflamación ocular. Ejemplos como los anteriores son extensivos a otras citocinas antiinflamatorias como la IL-4, IL-13, el factor estimulante de colonia granulocito –macrófago y el factor beta transformante del crecimiento (FβTC, y que serán mencionadas y descritas en el presente artículo de revisión, así como los factores que determinan ese comportamiento dual de las citocinas.SummaryThe anti-inflammatory cytokines which are recognized are theinterleukins (IL IL-4, IL-10, IL-13, interferon-alpha (IFN-α andbecause they are able to inhibit the release of pro inflammatorycytokines, to induce the production of IL-1 receptor antagonist (IL-raand the release of soluble TNF receptor and to limit some of the proinflammatory activities of IL-1 and TNF. However the events whichoccur during inflammation and sepsis are

  4. Miopatía inflamatoria con compromiso inicial de músculos respiratorios y artritis reumatoidea

    Directory of Open Access Journals (Sweden)

    Martín Hunter

    2014-10-01

    Full Text Available Las miopatías inflamatorias constituyen un grupo heterogéneo de enfermedades musculares adquiridas de presentación subaguda, crónica y a veces aguda. Las entidades clínicas más frecuentes son la dermatomiositis, la polimiositis, la miositis necrotizante autoinmune y la miositis por cuerpos de inclusión. Suelen presentarse con debilidad muscular con predominio proximal y simétrica, pero rara vez comprometen los músculos respiratorios. Presentamos el caso de una mujer de 39 años con miopatía inflamatoria inespecífica que presentó insuficiencia respiratoria secundaria a hipoventilación alveolar por debilidad muscular y requirió asistencia respiratoria mecánica. Respondió favorablemente y de forma rápida tras el tratamiento instaurado con inmunosupresores (corticoides y metotrexato e inmunoglobulina humana endovenosa. Se utilizó ventilación no invasiva como alternativa a la intubación orotraqueal con adecuada tolerancia.

  5. Acil coenzima-a sintetasas de cadena larga (acsls) como biomarcadores citoserológicos en enfermedades inflamatorias o autoinmunes

    OpenAIRE

    Matesanz, F; Fedetz, María; Ndagire, D.; Alcina, Antonio; Sabio, José Mario

    2010-01-01

    [ES] Uso de las Acil-Coenzima-A sintetasas de cadena larga (ACSL) como biomarcadores cito-serológicos de enfennedades inflamatorias o autoinmunes, y especialmente de lupus eritematoso sistémico, y método de obtención de datos útiles para el diagnóstico y seguimiento de dichas enfennedades.

  6. DIFFERENTIAL DIAGNOSIS OF ARTICULAR SYNDROME IN PSORIASIS

    OpenAIRE

    VAISOV ADKHAM SHAVKATOVICH; ALLAEVA MUASSAR JALALADINOVNA

    2015-01-01

    Articular syndrome in psoriasis is an urgent problem to date. By the way, not always articular syndrome in psoriasis is a manifestation of the disease. And so, below is a case osteochondropathy patient with psoriasis.

  7. La reacción inflamatoria en la fisiopatogenia de la obesidad

    Directory of Open Access Journals (Sweden)

    Ma. Victoria Domínguez García

    2012-01-01

    Full Text Available La obesidad se caracteriza por inflamación crónica. Entre los biomarcadores de inflamación encontramos las citocinas producidas por los adipocitos y los macrófagos: TNF - a, IL - 6, leptina, adiponectina y resistina. La relación adipocito-macrófago impacta la respuesta inflamatoria observada en la obesidad, la cual tiene una relación directa con la cantidad de macrófagos acumulados en el tejido adiposo blanco, que contribuye a la producción de mediadores de la inflamación. En este documento se hará una revisión de las principales moléculas involucradas en la generación de inflamación en la obesidad, y la relación entre macrófagos y adipocitos.

  8. Development of artificial articular cartilage.

    Science.gov (United States)

    Oka, M; Ushio, K; Kumar, P; Ikeuchi, K; Hyon, S H; Nakamura, T; Fujita, H

    2000-01-01

    Attempts have been made to develop an artificial articular cartilage on the basis of a new viewpoint of joint biomechanics in which the lubrication and load-bearing mechanisms of natural and artificial joints are compared. Polyvinyl alcohol hydrogel (PVA-H), 'a rubber-like gel', was investigated as an artificial articular cartilage and the mechanical properties of this gel were improved through a new synthetic process. In this article the biocompatibility and various mechanical properties of the new improved PVA-H is reported from the perspective of its usefulness as an artificial articular cartilage. As regards lubrication, the changes in thickness and fluid pressure of the gap formed between a glass plate and the specimen under loading were measured and it was found that PVA-H had a thicker fluid film under higher pressures than polyethylene (PE) did. The momentary stress transmitted through the specimen revealed that PVA-H had a lower peak stress and a longer duration of sustained stress than PE, suggesting a better damping effect. The wear factor of PVA-H was approximately five times that of PE. Histological studies of the articular cartilage and synovial membranes around PVA-H implanted for 8-52 weeks showed neither inflammation nor degenerative changes. The artificial articular cartilage made from PVA-H could be attached to the underlying bone using a composite osteochondral device made from titanium fibre mesh. In the second phase of this work, the damage to the tibial articular surface after replacement of the femoral surface in dogs was studied. Pairs of implants made of alumina, titanium or PVA-H on titanium fibre mesh were inserted into the femoral condyles. The two hard materials caused marked pathological changes in the articular cartilage and menisci, but the hydrogel composite replacement caused minimal damage. The composite osteochondral device became rapidly attached to host bone by ingrowth into the supporting mesh. The clinical implications of

  9. Characterization of patient’s inflammatory pulpal diseases. Caracterización de pacientes con afecciones pulpares inflamatorias

    Directory of Open Access Journals (Sweden)

    Ana Isabel Palenque Guillemí

    2008-06-01

    Full Text Available Background: Pulpal diseases are the most common entities in the dentist emergency consultation. For a correct indication of the treatment, it is very important to establish an accurate diagnosis. Objective: to characterize carrier patients with inflammatory pulpal disease. Methods: a descriptive, prospective cases series study which included 222 patients who came to the dentistry emergency consultation in Area I health policlinic. Age, type of disease, cause, more implicated teeth in the disease, visits, and previous treatments were the variables included. Results: there was no difference at all regarding sex. The most affected group of age was from 35 to 59 years old. The inferior molars were the most affected teeth. The main cause was dental cavities. Obturation was the previous treatment more presented. 46% of the patients attended the dentist in more than one occasion with the same process. Acute irreversible pulpitis was the most predominant pulpal disease. Conclusions: The most common pulpal diseases in our milieu was the acute irreversible pulpitis among the ages 35 and 59 years old.
    Fundamento: Las afecciones pulpares son las enfermedades que más llevan a los pacientes a una consulta de urgencia estomatológica. En estos casos, para una correcta indicación de tratamiento, es de suma importancia el establecimiento de un diagnóstico preciso. Objetivo: Caracterizar pacientes portadores de afectaciones pulpares inflamatorias. Métodos: Estudio descriptivo, prospectivo de serie de casos que incluyó 222 pacientes que asistieron a consulta de urgencia estomatológica en la policlínica del Área 1. Se incluyeron las siguientes variables: edad, tipo de afectación, causa, dientes más afectados, visitas y tratamientos previos. Resultados: No hubo diferencias en cuanto al sexo, el

  10. Propioceptores articulares y musculares

    OpenAIRE

    Vega, José A.

    1999-01-01

    La función de los mecanorreceptores de las articulaciones y músculos se considera asociada a la propiocepción. Sin embargo, existen evidencias de que la propiocepción no sólo depende del morfotipo de mecanorreceptor presente en dichos tejidos sino también de las propiedades de las neuronas sensitivas primarias y las fibras sensitivas asociadas a ellos, así como de su proyección sobre el asta posterior de la médula espinal. Este artículo resume las bases morfológicas de la propi...

  11. Uso de concentrados autólogos de plaquetas intraarticulares como coadyuvantes en el tratamiento quirúrgico de la rotura del ligamento cruzado anterior en una perra Use of intra-articular autologous platelet concentrates as coadjutants in the surgical treatment of a cranial cruciate ligament rupture in a bitch

    Directory of Open Access Journals (Sweden)

    RF Silva

    2011-01-01

    Full Text Available La ruptura del ligamento cruzado anterior (RLCA es uno de los principales problemas ortopédicos que producen cojera de los miembros posteriores en perros. A pesar de que este problema sea tratado quirúrgicamente el desarrollo y progresión de la osteoartritis son típicamente característicos. Se describe un caso de un perro que recibió inyecciones intraarticulares de concentrados autólogos de plaquetas (APCs durante el posoperatorio después de la reparación quirúrgica de una RLCA. Los resultados clínicos y radiográficos y el análisis de la marcha mediante plataforma de fuerza podrían potencialmente soportar la utilización posoperatoria de inyecciones intraarticulares de APCs como terapia coadyuvante en el tratamiento quirúrgico de la RLCA en perros.Cranial cruciate ligament rupture (CCLR is one of the main orthopedic problems that could produce hind limb lameness in dogs. Even though this problem can be surgically treated the development and progression of osteoarthritis are typical features. A case of a dog that received postoperative intra-articular injections of autologous platelet concentrates (APCs after the surgical reparation of a CCLR is described. The clinical, radiographic, and gait analysis results could potentially support the postoperative use of intra-articular injections of APCs as an adjunctive therapy in the CCLR surgical reparation.

  12. Postnatal development of articular cartilage

    NARCIS (Netherlands)

    Turnhout, van M.C.

    2010-01-01

    Articular cartilage (AC) is the thin layer of tissue that covers the ends of the bones in the synovial joints in mammals. Functional adult AC has depth-dependent mechanical properties that are not yet present at birth. These depth-dependent mechanical properties in adult life are the result of a dep

  13. Tratamiento cognitivo-conductual protocolizado en grupo de las enfermedades inflamatorias intestinales Protocolized cognitive-behavioural group therapy for inflammatory bowel disease

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    M. A. Díaz Sibaja

    2007-10-01

    Full Text Available Introducción: los pacientes con enfermedades inflamatorias intestinales presentan una mayor tasa de alteraciones psicopatológicas que la población normal, predominantemente ansiedad y depresión. Objetivo: el principal objetivo de este estudio fue diseñar, poner en práctica y validar un programa de tratamiento psicológico protocolizado, basado en el modelo de competencias y con una metodología psicoeducativa, en el que se incluyeron los siguientes módulos de intervención: información de la enfermedad, modelo de afrontamiento, solución de problemas, técnicas de relajación, entrenamiento en habilidades sociales, técnicas de distracción y técnicas de reestructuración cognitiva. Todas estás técnicas fueron adaptadas a la problemática característica de la enfermedad inflamatoria intestinal. Método: la muestra estuvo constituida por 57 pacientes diagnosticados de enfermedad inflamatoria intestinal de la Asociación de Enfermos de Crohn y Colitis Ulcerosa de España. La muestra se distribuyó aleatoriamente en dos grupos experimentales, 33 formaron parte del programa de tratamiento en grupo y 24 fueron asignados al grupo control en lista de espera. Las variables emocionales medidas fueron: ansiedad y depresión. Resultados: los resultados reflejaron una mejoría clínica y estadísticamente significativa en las variables de ansiedad (p Introduction: patients affected by inflammatory bowel disease have a high rate of psychiatric disorders such as depression and anxiety. Objective: the main goal of this study was to design, to put into practice, and to validate a protocolized psychological treatment program based on the coping model, and a psycho-educational methodology in which the following intervention modules were included- illness information, coping model, problem solving techniques, relaxation, social skill training, distraction, and cognitive restructuring techniques. All these techniques were adapted to the characteristic

  14. Hidrogeles biodegradables para la regeneración de cartílago articular

    OpenAIRE

    CANTÓ LORAS, PETRA

    2015-01-01

    [CAT] El cartílag articular és un teixit connectiu molt especialitzat present a les articulacions, que actua com amortidor suportant enormes carregues y proporcionant una superfície de lliscament de baixa fricció. Les patologies del cartílag articular suposen la pèrdua de l’estructura i de la funció del teixit i són una de les principals causes de discapacitat en persones grans. Es tracta d’un teixit avascular que presenta una capacitat d’autoreparació limitada. S’han desenvolupat nombrosos p...

  15. Incidencia de la cervicalgia de origen no articular en las actividades de la vida diaria.

    OpenAIRE

    Prioletto, Matías

    2010-01-01

    El presente trabajo de investigación se refiere a la “Incidencia de la cervicalgia de origen no articular en las actividades de la vida diaria”. La cervicalgia de origen no articular es una manifestación de dolor en las partes posterior y posteroloaterales del cuello, con o sin irradiación a las zonas y segmentos adyacentes, que encuentra su origen en las alteraciones de las partes blandas extraarticulares del raquis cervical. Considerando las relaciones entre las diferente...

  16. Importancia de los lípidos en el tratamiento nutricional de las patologías de base inflamatoria Importance of lipids in the nutritional treatment of inflammatory diseases

    Directory of Open Access Journals (Sweden)

    M. D. Mesa García

    2006-05-01

    Full Text Available En las últimas décadas, el conocimiento acerca de las propiedades antiinflamatorias de los lípidos ha facilitado el desarrollo de productos adaptados a la nutrición enteral y parenteral que han sido utilizados como terapia en numerosas enfermedades de base inflamatoria. En esta revisión se ofrece una visión general de los efectos de los lípidos utilizados en nutrición enteral, sobre ciertas patologías en cuya base están implicados procesos inflamatorios, como es el caso de las enfermedades inflamatorias intestinales, la aterosclerosis, la fibrosis pulmonar y la artritis reumatoide, entre otras. Durante los procesos inflamatorios se producen mediadores químicos, como los eicosanoides, a partir de ácidos grasos poliinsaturados presentes en las membranas celulares. La actividad inflamatoria de estas moléculas depende del ácido graso precursor. Los derivados del ácido araquidónico (de la serie n-6 tienen una elevada actividad proinflamatoria, mientras que los derivados del eicosapentaenoico (de la serie n-3 son débilmente inflamatorios. Así, al ingerir aceites de pescado, ricos en ácidos grasos n-3, aumenta el contenido de eicosapentaenoico y disminuye el araquidónico en las células del sistema inmune, por lo que se reduce la estimulación inflamatoria debido a la presencia de mediadores menos activos. Éste parece ser el mecanismo inmunomodulador fundamental de las dietas ricas en n-3. Asimismo, pero mediante mecanismos de acción diferentes, se ha demostrado que el ácido oleico disminuye la intensidad de algunos procesos inflamatorios, al disminuir la producción de mediadores quimiotácticos de inflamación. Podemos concluir que la suplementación de dietas para nutrición enteral con ácidos grasos poliinsaturados n-3 y oleico resulta beneficiosa para el tratamiento de ciertas patologías donde los procesos inflamatorios participan activamente en su desarrollo, mejorando su evolución y disminuyendo las dosis de f

  17. Arthoscopy La artroscopia y las lesiones articulares

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    Raúl J. Naranjo

    1991-01-01

    Full Text Available

    A general view is presented on arthroscopy; its history, indications and limitations are described and analyzed; its advantages in comparison with open surgery are emphasized.

    La artroscopia es un procedimiento que permite, mediante un instrumento óptico, evaluar el Interior de las cavidades articulares. Comenzó a desarrollarse como un procedimiento diagnóstico y pronto sus grandes ventajas frente a las exploraciones abiertas aceleraron el desarrollo de las técnicas y del instrumental. La minimización del trauma a los tejidos y la menor morbilidad posoperatoria permiten que la deambulación y la recuperación funcional sean precoces lo cual, sumado a un mejor resultado estético, ha colocado a la artroscopia como procedimiento de elección para el diagnóstico y el tratamiento de múltiples estados patológicos intraarticulares.

  18. Autophagy modulates articular cartilage vesicle formation in primary articular chondrocytes.

    Science.gov (United States)

    Rosenthal, Ann K; Gohr, Claudia M; Mitton-Fitzgerald, Elizabeth; Grewal, Rupinder; Ninomiya, James; Coyne, Carolyn B; Jackson, William T

    2015-05-22

    Chondrocyte-derived extracellular organelles known as articular cartilage vesicles (ACVs) participate in non-classical protein secretion, intercellular communication, and pathologic calcification. Factors affecting ACV formation and release remain poorly characterized; although in some cell types, the generation of extracellular vesicles is associated with up-regulation of autophagy. We sought to determine the role of autophagy in ACV production by primary articular chondrocytes. Using an innovative dynamic model with a light scatter nanoparticle counting apparatus, we determined the effects of autophagy modulators on ACV number and content in conditioned medium from normal adult porcine and human osteoarthritic chondrocytes. Healthy articular chondrocytes release ACVs into conditioned medium and show significant levels of ongoing autophagy. Rapamycin, which promotes autophagy, increased ACV numbers in a dose- and time-dependent manner associated with increased levels of autophagy markers and autophagosome formation. These effects were suppressed by pharmacologic autophagy inhibitors and short interfering RNA for ATG5. Caspase-3 inhibition and a Rho/ROCK inhibitor prevented rapamycin-induced increases in ACV number. Osteoarthritic chondrocytes, which are deficient in autophagy, did not increase ACV number in response to rapamycin. SMER28, which induces autophagy via an mTOR-independent mechanism, also increased ACV number. ACVs induced under all conditions had similar ecto-enzyme specific activities and types of RNA, and all ACVs contained LC3, an autophagosome-resident protein. These findings identify autophagy as a critical participant in ACV formation, and augment our understanding of ACVs in cartilage disease and repair.

  19. Inestabilidad crónica de tobillo: tratamiento mediante movilizaciones articulares y un programa de entrenamiento propioceptivo. Validación de la versión española del cuestionario "Cumberland Ankle Instability Tool"

    OpenAIRE

    Cruz-Díaz, David

    2013-01-01

    [ES] La inestabilidad crónica de tobillo CAI es una patología muy frecuente debido a que hasta un 80% de todos los pacientes que sufren un esguince de tobillo desarrollan secuelas relacionadas con la inestabilidad mecánica y funcional. Basándonos en la fisioterapia basada en la evidencia, el tratamiento de esta patología mediante la aplicación de técnicas de movilización articular y entrenamiento propioceptivo parece ser el más acertado para mejorar los déficits funcionales de estos pacientes...

  20. Tensorial electrokinetics in articular cartilage.

    Science.gov (United States)

    Reynaud, Boris; Quinn, Thomas M

    2006-09-15

    Electrokinetic phenomena contribute to biomechanical functions of articular cartilage and underlie promising methods for early detection of osteoarthritic lesions. Although some transport properties, such as hydraulic permeability, are known to become anisotropic with compression, the direction-dependence of cartilage electrokinetic properties remains unknown. Electroosmosis experiments were therefore performed on adult bovine articular cartilage samples, whereby fluid flows were driven by electric currents in directions parallel and perpendicular to the articular surface of statically compressed explants. Magnitudes of electrokinetic coefficients decreased slightly with compression (from approximately -7.5 microL/As in the range of 0-20% compression to -6.0 microL/As in the 35-50% range) consistent with predictions of microstructure-based models of cartilage material properties. However, no significant dependence on direction of the electrokinetic coupling coefficient was detected, even for conditions where the hydraulic permeability tensor is known to be anisotropic. This contrast may also be interpreted using microstructure-based models, and provides insights into structure-function relationships in cartilage extracellular matrix and physical mediators of cell responses to tissue compression. Findings support the use of relatively simple isotropic modeling approaches for electrokinetic phenomena in cartilage and related materials, and indicate that measurement of electrokinetic properties may provide particularly robust means for clinical evaluation of cartilage matrix integrity.

  1. Tratamiento farmacológico invasivo articular en la artrosis

    Directory of Open Access Journals (Sweden)

    D. Benítez Pareja

    2016-04-01

    Full Text Available La degeneración de las articulaciones axiales y periféricas va unida al propio envejecimiento del ser humano. Es responsable del dolor e impotencia funcional, y su fisiopatología es compleja y no del todo conocida. El denominador común en la artrosis es la degeneración del cartílago articular, junto a otras alteraciones secundarias. Su abordaje y manejo del dolor supone todo un reto para las Unidades del Dolor. Históricamente, el tratamiento intervencionista se llevó a cabo mediante infiltraciones a ciegas de diferentes sustancias que alivian el dolor. En la actualidad, la fluoroscopia, y más recientemente los ultrasonidos, son los métodos de elección para la guía de nuestros procedimientos. En cuanto a los fármacos utilizados más comúnmente en nuestras técnicas invasivas articulares destacaremos el ácido hialurónico (AH y el plasma rico en plaquetas (PRP. Los esteroides siguen siendo, en prácticamente todos los estudios, el estándar de oro o fármaco de referencia y sobre los que se comparan el resto de familias de fármacos. Una exploración exhaustiva del paciente, y un algoritmo de decisiones en cuanto a la decisión de realización de estas técnicas, se antoja necesario, adecuándose a cada lugar de trabajo.

  2. Intra-articular chondroma of the knee

    Energy Technology Data Exchange (ETDEWEB)

    Talwalkar, S.C.; Kambhampati, S.B.S.; Lang Stevenson, A.I. [Oldchurch Hospital, Romford, Essex (United Kingdom); Whitehouse, R. [Manchester University, Department of Radiology, Manchester (United Kingdom); Freemont, A. [University of Manchester, Department of Osteoarticular Pathology, Manchester (United Kingdom)

    2005-06-01

    Chondromas are tumours that develop in relation to the periosteum and, although they are common around the knee, most reports deal with soft tissue chondromas in para-articular locations or intracortical tumours in extra-articular regions. We report a rare case of an intra-articular chondroma in a 16-year-old boy of Asian origin developing in the region of the medial femoral condyle of the femur and extending into the femoral sulcus and the patellofemoral joint. (orig.)

  3. Efectos de la suplementación con membrana de cáscara de huevo sobre alteraciones tendinopáticas, articulares y variables mecánicas del tendón de Aquiles en deportistas de crossfit.

    OpenAIRE

    Jauregui Bidegain, Lucas

    2016-01-01

    Introducción: En la patología del tendón de aquiles es determinante el análisis de los factores mecánicos que ayudan a explicar el comportamiento del tendón, siendo de los más estudiados el stiffness lineal. No existe actualmente evidencia acerca de los efectos de la suplementación con membrana de cáscara de huevo en el stiffness. Únicamente se ha examinado mediante escalas de valoración subjetiva, por lo que la medición mediante ecografía del stiffness tendinoso como variable direc...

  4. Influencia del ambiente sobre la microbiota comensal del intestino de la rata: Implicaciones en la inflamación y en mecanismos sensoriales

    OpenAIRE

    Terán Ventura, Demetria Evangelina

    2009-01-01

    Evidencias recientes sugieren que la microbiota comensal del intestino constituye un factor dinámico que se relaciona activamente con el hospedador, mediante sistemas específicos de reconocimiento bacteriano, y participa en la regulación de funciones sensoriales, motoras y secretoras gastrointestinales. En particular, la microbiota comensal del intestino se ha implicado en los mecanismos fisiopatológicos de la enfermedad inflamatoria intestinal. Este trabajo de investigación tiene como objeti...

  5. Asma bronquial. Estado del arte.

    OpenAIRE

    Jorge Salazar

    2009-01-01

    Para infinidad de personas el asma es su problema de salud. El diagnóstico y el tratamiento varían dependiendo de la magnitud del compromiso y la causa que produce la reacción inflamatoria en los pulmones. La prevención y el tratamiento serán mejores si la población y los pacientes reconocen la necesidad de evitar determinadas exposiciones ambientales y el uso de medicamentos con la frecuencia necesaria. Ahora los médicos generales conocen mejor la fisiología y patología de esta enfermedad y ...

  6. La modulación de sentencias como medio para articular la oportunidad política de la Corte Constitucional colombiana. El caso de las parejas del mismo sexo

    Directory of Open Access Journals (Sweden)

    Juan Pablo Sarmiento E.

    2012-01-01

    Full Text Available En el marco de la trasformación que ha sufrido el régimen de derecho de familia hacia la inclusión de parejas del mismo sexo, la Corte Constitucional ha jugado un papel central y quizá solitario. En este artículo se analizan los principales fallos de la Corte Constitucional relativos a la unificación en el ámbito de protección a las parejas del mismo sexo, extrayendo de cada uno de ellos los elementos de creación jurisprudencial más relevantes. Con lo anterior se argumentará, entonces, que por medio de diversos instrumentos interpretativos, la Corte ha instrumentalizado sus fallos para ejercer su oportunidad política en dirección a la opinión prevalente por medio del condicionamiento de las sentencias de constitucionalidad.

  7. Estudio PET/TC en patología inflamatoria-infecciosa PET/CT in infectious and inflammatory pathology

    Directory of Open Access Journals (Sweden)

    Cecilia Carrera

    2012-06-01

    Full Text Available Objetivo. Demostrar la utilidad del examen PET/TC en patología inflamatoria-infecciosa. Materiales y Métodos. Evaluación retrospectiva (enero de 2009 - mayo de 2011 de los exámenes de tomografía por Emisión de Positrones/ Tomografía Computada (PET/TC, realizados en nuestra institución con un equipo híbrido SIEMENS-BIOGRAPH 16 (Siemens, Erlangen, Alemania. Se seleccionaron 5 pacientes. Resultados. Caso 1: paciente de 68 años de edad con fiebre de 6 meses de duración, fatiga y pérdida de peso. El examen reumatológico demostró disminución en pulsos radiales sin otros síntomas asociados. La paciente fue sometida a biopsia de arteria temporal. Ésta confirmó una arteritis de la arteria temporal y el estudio PET/TC demostró hipermetabolismo en la aorta torácica y ramas principales. Caso 2: paciente de 85 años con fiebre de origen desconocido (FOD y sospecha de osteomielitis de cadera. En contraposición, el PET/TC demostró un foco ávido de celulitis glútea y neumopatía. Caso 3: paciente de 35 años con fiebre vespertina. El PET/TC mostró múltiples adenomegalias ávidas por fluorodexosiglucosa (FDG en mediastino, axilas y retroperitoneo, y compromiso difuso esplénico asociado a calcificaciones. Se confirmó infección por citomegalovirus por inmuno-globulina G y M. Caso 4: paciente de 39 años con infección por HIV que consultó por hipercalcemia. El PET/TC mostró implantes de silicona en glúteos con proceso inflamatorio ávido asociado. Se confirmó por la biopsia de uno de ellos. Caso 5: paciente de 45 años con historia de cáncer de mama en control presentó en los últimos estudios tomográficos aumento del tamaño de los ganglios supraclaviculares y mediastínicos, y compromiso esplénico multifocal difuso. Estos resultaron ávidos en el examen PET/TC. Se confirmó el diagnóstico de sarcoidosis por el estudio anatomopatológico de un ganglio supraclavicular. Conclusiones. El PET/TC es un método no invasivo de

  8. Comportamiento de variables epidemiológicas en la neuropatía óptica inflamatoria infecciosa. Centro Oftalmológico de Holguín. 2009

    Directory of Open Access Journals (Sweden)

    Enma Estrella de la Torre-Rodríguez

    2012-01-01

    Full Text Available Abordó un estudio descriptivo del comportamiento de algunas variables epidemiológicas en un universo de 18 pacientes con neuropatía óptica inflamatoria en el Centro Oftalmológico de Holguín de enero a diciembre de 2009. La muestra estuvo representada por 12 pacientes con etiología infecciosa. El mayor número de pacientes se incluyó en el grupo de edad de 15 a 29 años. Las ocupaciones de riesgo más frecuentes fueron los estudiantes, las amas de casa y criadores de palomas. Los perros, gatos y cerdos fueron los animales más frecuentes en contacto con los casos. La etiología parasitaria representó el grupo más frecuente como causa de la neuropatía y el agente que predominó fue Bartonella henselae. Se recomendó realizar estudios que incluyan confirmación serológica y/o del agente causal en este grupo de enfermedades.

  9. Uso de anticuerpos contra proteínas extracelulares de bacterias de la microbiota intestinal como biomarcadores de Enfermedad Inflamatoria Intestinal

    OpenAIRE

    Hevia González, Arancha

    2012-01-01

    En este trabajo se pretende utilizar los niveles de anticuerpos dirigidos contra bacterias ingeridas con los alimentos, representantes de la microbiota intestinal, como posibles biomarcadores para uso en la Enfermedad Inflamatoria Intestinal con el fin de facilitar el desarrollo de mejores métodos de diagnóstico precoz.

  10. Acil Coenzima-A sintetasas de cadena larga (ACSLs) como biomarcadores cito-serológicos en enfermedades inflamatorias o autoinmunes

    OpenAIRE

    Matesanz, F; Fedetz, María; Ndagire, D.; Alcina, Antonio; Sabio, José Mario

    2010-01-01

    Uso de las Acil-Coenzima-A sintetasas de cadena larga (ACSL) como biomarcadores cito-serológicos de enfermedades inflamatorias o autoinmunes, y especialmente de lupus eritematoso sistémico, y método de obtención de datos útiles para el diagnóstico y seguimiento de dichas enfermedades. Venta

  11. Growth Factor Transgenes Interactively Regulate Articular Chondrocytes

    OpenAIRE

    Shi, Shuiliang; Mercer, Scott; Eckert, George J.; Trippel, Stephen B

    2013-01-01

    Adult articular chondrocytes lack an effective repair response to correct damage from injury or osteoarthritis. Polypeptide growth factors that stimulate articular chondrocyte proliferation and cartilage matrix synthesis may augment this response. Gene transfer is a promising approach to delivering such factors. No single growth factor gene is likely to optimize these cell functions, but multiple growth factor gene transfer remains unexplored. We tested the hypothesis that multiple growth fac...

  12. Characterization of cutaneous and articular sensory neurons

    OpenAIRE

    da Silva Serra, I.; Husson, Z.; Bartlett, J.D.; Smith, E.S.J.

    2016-01-01

    Background A wide range of stimuli can activate sensory neurons and neurons innervating specific tissues often have distinct properties. Here, we used retrograde tracing to identify sensory neurons innervating the hind paw skin (cutaneous) and ankle/knee joints (articular), and combined immunohistochemistry and electrophysiology analysis to determine the neurochemical phenotype of cutaneous and articular neurons, as well as their electrical and chemical excitability. Results Immunohistoche...

  13. Imaging of intra-articular osteoid osteoma

    Energy Technology Data Exchange (ETDEWEB)

    Allen, S.D.; Saifuddin, A. E-mail: asaifuddin@aol.com

    2003-11-01

    Intra-articular osteoid osteoma accounts for approximately 13% of all osteoid osteomas and presents as a monoarthropathy. Radiographs commonly do not identify the nidus, and in this event, MRI is likely to be the next imaging investigation. MRI may show a variety of appearances depending upon the age of the lesion. This article illustrates the imaging features of intra-articular osteoid osteoma, with emphasis on MRI. CT remains the investigation of choice for identifying the nidus.

  14. Hallazgo de una leucorrea vaginal de carácter no inflamatoria en hembras bovinas. I. Examen macroscópico y microscópico (Finding of a vaginal leucorrhea of non-inflammatory character in bovine females. I. Macroscopic and microscopic examination

    Directory of Open Access Journals (Sweden)

    Luis O. Alba Gómez; Guadalupe Hernández Breto.

    2005-07-01

    Full Text Available Con el objetivo de demostrar la existencia de una leucorrea vaginal no inflamatoria en hembras bovinas, se examinaron macroscópicamente los órganos genitales de 350 novillas y 800 vacas Cebú comercial y mestizas Holstein x Cebú sacrificadas en el matadero. Se colectaron secreciones leucorréicas en las vaginas o cuernos uterinos del 12 y 20% de las novillas y vacas respectivamente. Ninguno de los órganos genitales de estos animales mostraron signos de inflamación en sus mucosas. El examen histológico se realizó en 80 muestras de vagina y de cuernos uterinos leucorréicos y solamente el 6,3% de los cortes histológicos de vaginas y el 8,8% de los de úteros presentaron lesiones microscópicas leves. El pH vaginal promedió 6,85  0,2. No se encontraron diferencias en el espectro electroforético entre las secreciones mucosas y las leucorréicas. Tampoco se encontraron células inflamatorias en los frotis teñidos. Los resultados indican que las secreciones leucorréicas observadas no son verdaderos exudados, sino secreciones mucosas modificadas que no tienen un origen inflamatorio.

  15. Growth factor transgenes interactively regulate articular chondrocytes.

    Science.gov (United States)

    Shi, Shuiliang; Mercer, Scott; Eckert, George J; Trippel, Stephen B

    2013-04-01

    Adult articular chondrocytes lack an effective repair response to correct damage from injury or osteoarthritis. Polypeptide growth factors that stimulate articular chondrocyte proliferation and cartilage matrix synthesis may augment this response. Gene transfer is a promising approach to delivering such factors. Multiple growth factor genes regulate these cell functions, but multiple growth factor gene transfer remains unexplored. We tested the hypothesis that multiple growth factor gene transfer selectively modulates articular chondrocyte proliferation and matrix synthesis. We tested the hypothesis by delivering combinations of the transgenes encoding insulin-like growth factor I (IGF-I), fibroblast growth factor-2 (FGF-2), transforming growth factor beta1 (TGF-β1), bone morphogenetic protein-2 (BMP-2), and bone morphogenetic protien-7 (BMP-7) to articular chondrocytes and measured changes in the production of DNA, glycosaminoglycan, and collagen. The transgenes differentially regulated all these chondrocyte activities. In concert, the transgenes interacted to generate widely divergent responses from the cells. These interactions ranged from inhibitory to synergistic. The transgene pair encoding IGF-I and FGF-2 maximized cell proliferation. The three-transgene group encoding IGF-I, BMP-2, and BMP-7 maximized matrix production and also optimized the balance between cell proliferation and matrix production. These data demonstrate an approach to articular chondrocyte regulation that may be tailored to stimulate specific cell functions, and suggest that certain growth factor gene combinations have potential value for cell-based articular cartilage repair.

  16. Human stem cells and articular cartilage regeneration.

    Science.gov (United States)

    Inui, Atsuyuki; Iwakura, Takashi; Reddi, A Hari

    2012-11-05

    The regeneration of articular cartilage damaged due to trauma and posttraumatic osteoarthritis is an unmet medical need. Current approaches to regeneration and tissue engineering of articular cartilage include the use of chondrocytes, stem cells, scaffolds and signals, including morphogens and growth factors. Stem cells, as a source of cells for articular cartilage regeneration, are a critical factor for articular cartilage regeneration. This is because articular cartilage tissue has a low cell turnover and does not heal spontaneously. Adult stem cells have been isolated from various tissues, such as bone marrow, adipose, synovial tissue, muscle and periosteum. Signals of the transforming growth factor beta superfamily play critical roles in chondrogenesis. However, adult stem cells derived from various tissues tend to differ in their chondrogenic potential. Pluripotent stem cells have unlimited proliferative capacity compared to adult stem cells. Chondrogenesis from embryonic stem (ES) cells has been studied for more than a decade. However, establishment of ES cells requires embryos and leads to ethical issues for clinical applications. Induced pluripotent stem (iPS) cells are generated by cellular reprogramming of adult cells by transcription factors. Although iPS cells have chondrogenic potential, optimization, generation and differentiation toward articular chondrocytes are currently under intense investigation.

  17. Human Stem Cells and Articular Cartilage Regeneration

    Directory of Open Access Journals (Sweden)

    A. Hari Reddi

    2012-11-01

    Full Text Available  The regeneration of articular cartilage damaged due to trauma and posttraumatic osteoarthritis is an unmet medical need. Current approaches to regeneration and tissue engineering of articular cartilage include the use of chondrocytes, stem cells, scaffolds and signals, including morphogens and growth factors. Stem cells, as a source of cells for articular cartilage regeneration, are a critical factor for articular cartilage regeneration. This is because articular cartilage tissue has a low cell turnover and does not heal spontaneously. Adult stem cells have been isolated from various tissues, such as bone marrow, adipose, synovial tissue, muscle and periosteum. Signals of the transforming growth factor beta superfamily play critical roles in chondrogenesis. However, adult stem cells derived from various tissues tend to differ in their chondrogenic potential. Pluripotent stem cells have unlimited proliferative capacity compared to adult stem cells. Chondrogenesis from embryonic stem (ES cells has been studied for more than a decade. However, establishment of ES cells requires embryos and leads to ethical issues for clinical applications. Induced pluripotent stem (iPS cells are generated by cellular reprogramming of adult cells by transcription factors. Although iPS cells have chondrogenic potential, optimization, generation and differentiation toward articular chondrocytes are currently under intense investigation.

  18. Fisiopatología celular de la osteoartritis: el condrocito articular como protagonista = Osteoarthritis cellular pathophysiology: The articular chondrocyte as a central player

    OpenAIRE

    Sánchez Naranjo, Julio César; López Zapata, Diego Fernando

    2011-01-01

    La osteoartritis es una de las enfermedades más prevalentes y que más discapacidad produce en todo el mundo, lo que ocasiona costos altos para el paciente y la sociedad. En años recientes se ha venido obteniendo información importante sobre el funcionamiento normal del condrocito, la única célula presente en el cartílago articular y responsable de la síntesis de matriz extracelular. El condrocito responde a las condiciones fluctuantes del medio, generadas por los cambios de presión, modifican...

  19. Estudio de la morfología de la superficie articular de injertos osteocondriales frescos, congelados y criopreservados empleando un microscopio electrónico de barrido ambiental.

    OpenAIRE

    Carbonell Di Mola, José Antonio

    2002-01-01

    [spa] La superficie articular es un indicador pronóstico del éxito del implante osteocondral criopreservado y podría constituirse en parámetro para el control de la calidad del proceso de almacenamiento de este tipo de tejidos. Objetivos: fotografiar la superficie articular de injertos osteocondrales frescos, crear un sistema de clasificación de los principales aspectos morfológicos de la superficie condral de los injertos y determinar los cambios que en ellos produce la congelación a - 40 ºC...

  20. Articular chondrocyte metabolism and osteoarthritis

    Energy Technology Data Exchange (ETDEWEB)

    Leipold, H.R.

    1989-01-01

    The three main objectives of this study were: (1) to determine if depletion of proteoglycans from the cartilage matrix that occurs during osteoarthritis causes a measurable increase of cartilage proteoglycan components in the synovial fluid and sera, (2) to observe what effect intracellular cAMP has on the expression of matrix components by chondrocytes, and (3) to determine if freshly isolated chondrocytes contain detectable levels of mRNA for fibronectin. Canine serum keratan sulfate and hyaluronate were measured to determine if there was an elevation of these serum glycosaminoglycans in a canine model of osteoarthritis. A single intra-articular injection of chymopapain into a shoulder joint increased serum keratan sulfate 10 fold and hyaluronate less than 2 fold in 24 hours. Keratan sulfate concentrations in synovial fluids of dogs about one year old were unrelated to the presence of spontaneous cartilage degeneration in the joints. High keratan sulfate in synovial fluids correlated with higher keratan sulfate in serum. The mean keratan sulfate concentration in sera of older dogs with osteoarthritis was 37% higher than disease-free controls, but the difference between the groups was not statistically significant. Treatment of chondrocytes with 0.5 millimolar (mM) dibutyryl cAMP (DBcAMP) caused the cells to adopt a more rounded morphology. There was no difference between the amount of proteins synthesized by cultures treated with DBcAMP and controls. The amount of fibronectin (FN) in the media of DBcAMP treated cultures detected by an ELISA was specifically reduced, and the amount of {sup 35}S-FN purified by gelatin affinity chromatography decreased. Moreover, the percentage of FN containing the extra domain. A sequence was reduced. Concomitant with the decrease in FN there was an increase in the concentration of keratan sulfate.

  1. Avaliação do impacto da aplicação de manobra de hiperinsuflação pulmonar sobre a resposta inflamatória sistêmica e colapso pulmonar em pacientes submetidos a procedimentos cirúrgicos sob ventilação espontânea Evaluación del impacto de la aplicación de maniobra de hiperinsuflación pulmonar sobre la respuesta inflamatoria sistémica y colapso pulmonar en pacientes sometidos a procedimientos quirúrgicos bajo ventilación espontánea Assessing the impact of lung hyperinflation maneuver on systemic inflammatory response and lung collapse in patients undergoing surgeries under spontaneous ventilation

    Directory of Open Access Journals (Sweden)

    Luiz Marcelo Sá Malbouisson

    2010-06-01

    puede conllevar a una respuesta inflamatoria sistémica de origen pulmonar. Los objetivos de este estudio fueron comprobar el impacto de la aplicación de MHP sobre la respuesta inflamatoria sistémica y sobre la estructura pulmonar en pacientes sometidos a la anestesia subaracnoidea. MÉTODO: Después de la aprobación del Comité de Ética institucional y de la obtención del consentimiento informado, 20 pacientes sometidos a procedimientos quirúrgicos de pequeño y mediano porte, se separaron aleatoriamente en dos grupos: 1 control (GC y 2 MHP (GMHP. Una hora después de la instalación del bloqueo espinal, fue realizada MHP en el GMHP aplicando una presión positiva en las vías aéreas en los niveles (BIPAP con una presión espiratoria de 20 cmH2O y una presión inspiratoria de 20 cmH2O durante 1 a 2 minutos. FNTα, IL-1, IL-6, IL-8, IL-10 y IL-12 se midieron en la sangre a través de la técnica de citometría de flujo en los momentos basal, 90, 180 y 780 minutos. Los volúmenes y el peso pulmonares fueron computados a partir de tomografías computadorizadas obtenidas inmediatamente después de la operación. RESULTADOS: La aplicación de MHP resultó en una reducción de la fracción del parénquima pulmonar no aerado (7,5 ± 4,3% en el Grupo Control vs 4 ± 2,1% en el Grupo MHP, p = 0,02, sin alteraciones en los volúmenes pulmonares. Se observó una elevación progresiva en los valores plasmáticos de las interleucinas IL-1, IL-6, IL-8 y IL-10, similar en los dos grupos. Los niveles plasmáticos de FNTBACKGROUND AND OBJECTIVES: Lung hyperinflation maneuvers (LHM reverse intraoperative atelectasis; however, they can lead to pulmonary-induced systemic inflammatory response. The objective of this study was to determine the impact of LHM on systemic inflammatory response and lung structure in patients undergoing subarachnoid block. METHODS: After approval by the Ethics Committee of the institution and signing the informed consent, 20 patients undergoing small and

  2. Development of artificial articular cartilage

    Indian Academy of Sciences (India)

    Biswajit Bera

    2009-10-01

    The present study describes the development of artificial articular cartilage on the basis of mimicking structural gel properties and mechanical gel properties of natural articular cartilage. It is synthesized from PVA/Si nanocomposite containing 20% Tetra ethoxy silane (TEOS) by sol–gel method. Mechanical strength of Poly(vinyl alcohol), PVA is improved up to 35 MPa. Manufacturing method is adopted considering colloidal stability of nano silica particle in PVA sol at specific pH = 1. An adhesive is also prepared from PVA/Si nanocomposite containing 40% TEOS for firm attachment of artificial articular cartilage on underlying bone with high bond strength.

  3. Impacto de la nutrición en la evolución de la enfermedad inflamatoria intestinal Impact of nutritional treatment in the evolution of inflammatory bowel disease

    Directory of Open Access Journals (Sweden)

    M.ª D. Ballesteros Pomar

    2010-04-01

    Full Text Available La enfermedad inflamatoria intestinal es una entidad de etiopatogenia aún no bien conocida, con importantes implicaciones nutricionales y metabólicas, por la alta prevalencia de malnutrición que conlleva; por la posible implicación de factores dietéticos en su patogenia; y por la hipótesis de que la intervención nutricional pudiera ser un tratamiento primario de la enfermedad. Algunos nutrientes, además de su función exclusivamente nutricional, podrían inducir un bajo estímulo antigénico, regular respuestas inflamatorias e inmunológicas y estimular el trofismo de la mucosa intestinal. La evidencia disponible actual apoya el empleo de nutrición enteral en enfermedad de Crohn como terapia primaria en adultos si el tratamiento con corticoides no es posible (fracaso o contraindicación (grado de recomendación A o bien en terapia combinada con fármacos en pacientes malnutridos y estenosis inflamatoria del intestino. En los pacientes en remisión clínica duradera no se ha demostrado beneficio de la nutrición enteral o suplementos en ausencia de déficits nutricionales. No se recomienda el uso de fórmulas elementales ni modificadas (glutamina, ácidos grasos omega 3. En colitis ulcerosa, no se ha demostrado la influencia de la nutrición sobre la actividad de la enfermedad, aunque disponemos de datos prometedores sobre el papel de los ácidos grasos w3 con cubierta entérica y de un posible papel de los probióticos. En el tratamiento y profilaxis de la pouchitis crónica, el empleo de probióticos puede tener un papel (VSL#3. La nutrición debe considerarse un componente integral en el manejo de los pacientes con EII.Inflammatory bowel disease is an entity with not wellknown pathogenesis, and important nutritional and metabolic implications because of the high prevalence of malnutrition, the possible implication of dietary factors in its pathogenesis and because of the hypothesis that nutritional intervention could be a primary

  4. Luxación intra-articular de rótula Intra-articular dislocation of patella

    Directory of Open Access Journals (Sweden)

    S. García-Mata

    2006-08-01

    Full Text Available Presentamos el caso de una paciente de 73 años de edad que, tras una caída accidental en las escaleras, sufrió una luxación intra-articular aguda de rótula de la rodilla izquierda. Esta luxación excepcional presentaba incarceración del polo proximal rotuliano en el surco intercondíleo femoral. Se realizó reducción bajo anestesia general debido al dolor y contractura muscular en los intentos de reducción sin anestesia. Realizamos revision quirúrgica que descartó lesiones asociadas del aparato extensor, partes blandas, estructuras articulares y osteofitos. Presentaba artrosis avanzada, sobre todo fémoro-patelar. Tras dos semanas de inmovilización reinició movilidad. No ha presentado recidiva u otro tipo de complicaciones.The case of a patient aged 73 years who, following an accidental fall on a staircase, suffered an acute intraarticular dislocation in the left knee-cap is presented. This exceptional dislocation presented incarceration of the proximal kneecap pole in the femoral intercondylar groove. A reduction was carried out under general anaesthetic due to pain and muscular contraction in the attempts at reduction without anaesthetic. We made a surgical check that ruled out associated lesions to the extensor apparatus, soft parts, joint structures and osteophytes. The patient presented advanced arthrosis, above all femoral-patellar. Following two weeks of immobilisation, the patient restarted mobility. There has been no relapse or other type of complication.

  5. Preparation of Articular Cartilage Specimens for Scanning Electron Microscopy.

    Science.gov (United States)

    Stupina, T A

    2016-08-01

    We developed and adapted a technology for preparation of articular cartilage specimens for scanning electron microscopy. The method includes prefixation processing, fixation, washing, and dehydration of articular cartilage specimens with subsequent treatment in camphene and air-drying. The technological result consists in prevention of deformation of the articular cartilage structures. The method is simpler and cheaper than the known technologies. PMID:27591865

  6. Advanced Strategies for Articular Cartilage Defect Repair

    Directory of Open Access Journals (Sweden)

    Fergal J. O'Brien

    2013-02-01

    Full Text Available Articular cartilage is a unique tissue owing to its ability to withstand repetitive compressive stress throughout an individual’s lifetime. However, its major limitation is the inability to heal even the most minor injuries. There still remains an inherent lack of strategies that stimulate hyaline-like articular cartilage growth with appropriate functional properties. Recent scientific advances in tissue engineering have made significant steps towards development of constructs for articular cartilage repair. In particular, research has shown the potential of biomaterial physico-chemical properties significantly influencing the proliferation, differentiation and matrix deposition by progenitor cells. Accordingly, this highlights the potential of using such properties to direct the lineage towards which such cells follow. Moreover, the use of soluble growth factors to enhance the bioactivity and regenerative capacity of biomaterials has recently been adopted by researchers in the field of tissue engineering. In addition, gene therapy is a growing area that has found noteworthy use in tissue engineering partly due to the potential to overcome some drawbacks associated with current growth factor delivery systems. In this context, such advanced strategies in biomaterial science, cell-based and growth factor-based therapies that have been employed in the restoration and repair of damaged articular cartilage will be the focus of this review article.

  7. Juxta-articular myxoma of the palm

    OpenAIRE

    Irving, AK; Gwynne-Jones, D; Osipov, V.; Nicholson, ML

    2012-01-01

    We present a case of juxta-articular myxoma of the hand in a 63-year-old man with an enlarging mass over the palmar radial aspect of the index metacarpophalangeal joint. Usually seen in men, this benign soft tissue tumour is normally found around large joints. To our knowledge this is the first reported case in the hand.

  8. Monografía sobre el intestino grueso : enfermedades inflamatorias intestinales

    OpenAIRE

    López Mora, Gema

    2014-01-01

    El intestino grueso es un órgano con forma de tubo, de aproximadamente un metro y medio de largo, que forma parte del sistema digestivo. Consta de varias partes llamadas ciego (con su apéndice vermiforme); colon, en donde ponemos diferenciar colon ascendente, transverso, descendente y sigmoide; recto y ano. Sus paredes están cubiertas por un epitelio simple cilíndrico, que no presenta pliegues ni vellosidades y, que forma numerosas glándulas mucosas tubulares llamadas criptas de Lierberkühn, ...

  9. Vitiligo, con énfasis en su variante inflamatoria Vitiligo, with emphasis in its inflammatory variant

    OpenAIRE

    C I Vera; Díaz, M. C.

    2009-01-01

    El vitiligo inflamatorio es un trastorno melanocitopénico adquirido, de baja frecuencia con características clínicas e histológicas propias. Ocurre en ambos sexos, a cualquier edad. Su fisiopatogenia parece involucrar mecanismos autoinmunes. En su evolución es frecuente la desaparición del componente inflamatorio que resulta en una mácula hipopigmentada clásica; a ello debe anticiparse también nuestra elección terapéutica. Se describe un paciente con vitiligo inflamatorio de resolución espont...

  10. Dolor osteomuscular y reumatológico Osteomuscular and rheumatic pain

    OpenAIRE

    J. L. Rodríguez-Hernández

    2004-01-01

    Dentro del dolor osteomuscular y reumatológico encontramos varias entidades. La osteoartrosis u osteoartritis es una enfermedad inflamatoria articular que produce alteraciones estructurales en el cartílago hialino articular y esclerosis del hueso subcondral. Tiene origen multifactorial con síntomas localizados en las articulaciones afectadas y su tratamiento va dirigido al dolor, inflamación, funcionalidad y prevención de deformidades. La artritis reumatoide es una enfermedad crónica que afec...

  11. Efectividad y seguridad de la viscosuplementación con dosis única de ácido hialurónico estabilizado de origen no animal (NASHA para el tratamiento del dolor secundario a gonartrosis Efficacy and safety of single intra-articular injection of non-animal stabilized hyaluronic acid (NASHA for gonarthrosis pain treatment

    Directory of Open Access Journals (Sweden)

    M. M. Monerris Tabasco

    2012-10-01

    Full Text Available Objetivos: valorar la efectividad y seguridad de la inyección de dosis única de ácido hialurónico estabilizado de origen no animal (NASHA para el tratamiento del dolor secundario a gonartrosis. Material y métodos: se han incluido un total de 37 pacientes afectos de gonartrosis diagnosticada previamente por el Traumatólogo/Reumatólogo en los que ha fracasado el tratamiento conservador y/o que no son tributarios de tratamiento quirúrgico. Se les ha efectuado una infiltración en dosis única de ácido hialurónico estabilizado no animal (NASHA, Durolane®. Se han recogido datos demográficos, efectos adversos e intensidad del dolor (valorada mediante escala verbal numérica y puntuación en los cuestionarios Western Ontario and McMaster Universities Osteoartritis Index (WOMAC y Barthel -basal, a las 2 semanas, a los 3 meses y a los 6 meses de la infiltración-. Se ha considerado efectiva una disminución ≥ 30% durante más de 3 meses en las puntuaciones basales. Resultados: los pacientes son mayoritariamente mujeres (83,8%, con una edad media de 72,8 años (desviación típica -S- = 8,4 e índice de masa corporal medio (IMC 31,9 (S = 6,9. La rodilla infiltrada es izquierda en el 51,7%, derecha en el 41,4% y bilateral en el 6,9% de los pacientes. Han presentado efectos adversos en forma de artralgias bien toleradas el 11,1% de los pacientes. En base a los resultados, la infiltración con dosis única de ácido hialurónico estabilizado de origen no animal (NASHA por gonalgia de origen artrósico provoca una reducción estadísticamente significativa en la intensidad del dolor (escala verbal numérica y WOMAC y de la capacidad funcional (WOMAC en todos los intervalos valorados. Se ha producido una reducción ≥ 30% en la intensidad del dolor en el 54% (valor que coincide con la reducción en la puntuación WOMAC de dolor, en la rigidez en el 62,1% y en la capacidad funcional en el 48,6% de los pacientes. Conclusiones: en nuestros

  12. Articular hypermobility simulating chronic rheumatic disease.

    OpenAIRE

    Lewkonia, R M; Ansell, B M

    1983-01-01

    A retrospective survey of 54 children with symptoms associated with articular hypermobility showed only occasional recognition of the syndrome before referral; some patients had been treated for inflammatory arthritis. Of the patients, 78% were girls; their mean age at onset of symptoms (11 years) was 3 years older than the mean age of the boys. Hypermobility was localised rather than generalised in most patients, and this may be an important reason for the apparent under recognition of the s...

  13. Articular cartilage collagen: an irreplaceable framework?

    OpenAIRE

    Eyre, D. R.; Weis, M A; J-J Wu

    2006-01-01

    Adult articular cartilage by dry weight is two-thirds collagen. The collagen has a unique molecular phenotype. The nascent type II collagen fibril is a heteropolymer, with collagen IX molecules covalently linked to the surface and collagen XI forming the filamentous template of the fibril as a whole. The functions of collagens IX and XI in the heteropolymer are far from clear but, evidently, they are critically important since mutations in COLIX and COLXI genes can result in chondrodysplasia ...

  14. Oxygen, nitric oxide and articular cartilage

    OpenAIRE

    Fermor, B.; Christensen, S. E.; I Youn; J M Cernanec; C M Davies; Weinberg, J. B.

    2007-01-01

    Molecular oxygen is required for the production of nitric oxide (NO), a pro-inflammatory mediator that is associated with osteoarthritis and rheumatoid arthritis. To date there has been little consideration of the role of oxygen tension in the regulation of nitric oxide production associated with arthritis. Oxygen tension may be particularly relevant to articular cartilage since it is avascular and therefore exists at a reduced oxygen tension. The superficial zone exists at approximately 6% O...

  15. Polarized IR microscopic imaging of articular cartilage

    Science.gov (United States)

    Ramakrishnan, Nagarajan; Xia, Yang; Bidthanapally, Aruna

    2007-08-01

    The objective of this spectroscopic imaging study is to understand the anisotropic behavior of articular cartilage under polarized infrared radiation at 6.25 µm pixel resolution. Paraffin embedded canine humeral cartilage-bone blocks were used to obtain 6 µm thick tissue sections. Two wire grid polarizers were used to manipulate the polarization states of IR radiation by setting them for various polarizer/analyzer angles. The characteristics of the major chemical components (amide I, amide II, amide III and sugar) of articular cartilage were investigated using (a) a polarizer and (b) a combination of a polarizer and an analyzer. These results were compared to those obtained using only an analyzer. The infrared anisotropy (variation in infrared absorption as a function of polarization angles) of amide I, amide II and amide III bands correlates with the orientation of collagen fibrils along the tissue depth in different histological zones. An 'anisotropic flipping' region of amide profiles indicates the possibility of using Fourier transform infrared imaging (FTIRI) to determine the histological zones in cartilage. Cross-polarization experiment indicates the resolution of overlapping peaks of collagen triple helix and/or proteoglycan in articular cartilage.

  16. Oxygen, nitric oxide and articular cartilage

    Directory of Open Access Journals (Sweden)

    B Fermor

    2007-04-01

    Full Text Available Molecular oxygen is required for the production of nitric oxide (NO, a pro-inflammatory mediator that is associated with osteoarthritis and rheumatoid arthritis. To date there has been little consideration of the role of oxygen tension in the regulation of nitric oxide production associated with arthritis. Oxygen tension may be particularly relevant to articular cartilage since it is avascular and therefore exists at a reduced oxygen tension. The superficial zone exists at approximately 6% O2, while the deep zone exists at less than 1% O2. Furthermore, oxygen tension can alter matrix synthesis, and the material properties of articular cartilage in vitro.The increase in nitric oxide associated with arthritis can be caused by pro-inflammatory cytokines and mechanical stress. Oxygen tension significantly alters endogenous NO production in articular cartilage, as well as the stimulation of NO in response to both mechanical loading and pro-inflammatory cytokines. Mechanical loading and pro-inflammatory cytokines also increase the production of prostaglandin E2 (PGE2. There is a complex interaction between NO and PGE2, and oxygen tension can alter this interaction. These findings suggest that the relatively low levels of oxygen within the joint may have significant influences on the metabolic activity, and inflammatory response of cartilage as compared to ambient levels. A better understanding of the role of oxygen in the production of inflammatory mediators in response to mechanical loading, or pro-inflammatory cytokines, may aid in the development of strategies for therapeutic intervention in arthritis.

  17. Fisiopatología celular de la osteoartritis: el condrocito articular como protagonista = Osteoarthritis cellular pathophysiology: The articular chondrocyte as a central player

    Directory of Open Access Journals (Sweden)

    Sánchez Naranjo, Julio César

    2011-06-01

    Full Text Available La osteoartritis es una de las enfermedades más prevalentes y que más discapacidad produce en todo el mundo, lo que ocasiona costos altos para el paciente y la sociedad. En años recientes se ha venido obteniendo información importante sobre el funcionamiento normal del condrocito, la única célula presente en el cartílago articular y responsable de la síntesis de matriz extracelular. El condrocito responde a las condiciones fluctuantes del medio, generadas por los cambios de presión, modificando su composición iónica y alterando el transporte de solutos y agua en su membrana. Esta capacidad de respuesta es clave para el mantenimiento de la matriz extracelular y, por ende, de un cartílago funcional. Diversos factores relacionados con enfermedades crónicas metabólicas inician una cascada de eventos que termina con una respuesta inadecuada del condrocito ante la carga mecánica, lo cual lleva a un predominio del catabolismo de la matriz y a un cartílago defectuoso que es la base del desarrollo de la osteoartritis. En este proceso están implicadas diversas citocinas y hormonas que afectan la homeostasis del cartílago y que pueden constituirse en blancos terapéuticos prometedores.

  18. Vitiligo, con énfasis en su variante inflamatoria Vitiligo, with emphasis in its inflammatory variant

    Directory of Open Access Journals (Sweden)

    C I Vera

    2009-03-01

    Full Text Available El vitiligo inflamatorio es un trastorno melanocitopénico adquirido, de baja frecuencia con características clínicas e histológicas propias. Ocurre en ambos sexos, a cualquier edad. Su fisiopatogenia parece involucrar mecanismos autoinmunes. En su evolución es frecuente la desaparición del componente inflamatorio que resulta en una mácula hipopigmentada clásica; a ello debe anticiparse también nuestra elección terapéutica. Se describe un paciente con vitiligo inflamatorio de resolución espontánea, junto a una revisión crítica de la bibliografía. Se discute si representa una variante de vitiligo vulgar o una entidad independiente.Inflammatory vitiligo is a low frequency melanocytopenic acquired entity with clinical and histological features of it 's own. It occurs in both sexes at any age. Fisiopathogenesis may involve autoimmune mechanisms. It's evolution most commonly ends up in a classic hypopigmented maculae, therefore our therapeutic choice has to be oriented to this outcome. A patient with inflammatory vitiligo is described along a critical review of the literature. It is discussed if it represents a variant of common vitiligo or an independent entity.

  19. Is safety infliximab during pregnancy in patients with inflammatory bowel disease? ¿El tratamiento con infliximab es seguro durante el embarazo en pacientes con enfermedad inflamatoria intestinal?

    Directory of Open Access Journals (Sweden)

    Federico Argüelles-Arias

    2012-02-01

    foetus. In fact, in our study and in some cases, its withdrawal may lead to a worsening of the disease. However, further control studies are required with larger samples to obtain more representative findings.Introducción: la enfermedad inflamatoria intestinal (EII es un trastorno crónico que debuta en la mayoría de los casos durante la edad reproductiva. Existen pocos datos sobre la seguridad durante el embarazo de los tratamientos disponibles, entre ellos los denominados biológicos, y estos están basados en resultados de casos esporádicos. Objetivos: determinar la seguridad del tratamiento con infliximab (IFX durante el embarazo en mujeres con EII. Un segundo objetivo es observar el efecto que sobre la actividad de la enfermedad tiene el abandono del tratamiento. Material y métodos: se trata de un estudio retrospectivo en el que se incluyeron mujeres con EII embarazadas y que estaban en tratamiento con IFX durante el embarazo. Se incluyeron en el estudio a 5 hospitales de España. La actividad de la enfermedad se midió según el CDAI en la enfermedad de Crohn (EC y la de la colitis ulcerosa (CU según el índice de Truelove-Witts en cada trimestre del embarazo. La edad gestacional, el peso y las enfermedades del feto se determinaron al nacimiento. Resultados: se incluyeron doce mujeres con una edad media de 29 años, 4 diagnosticadas de CU y 8 de EC, con una duración media de la enfermedad de 7 años. Todas salvo una, que se diagnosticó durante el embarazo estaban siendo tratadas con IFX en el momento de la concepción. Seis pacientes recibieron el tratamiento de forma ininterrumpida durante todo el embarazo, 2 suspendieron el tratamiento de forma voluntaria y a tres se les suspendió el tratamiento en el tercer trimestre. Recibieron una dosis media de IFX de 400 mg cada 8 semanas. De las 6 pacientes que recibieron tratamiento continuo, el 50% se mantuvo en remisión. De las pacientes que abandonaron el tratamiento, un 83,3% (todas menos una presentaron un

  20. A population-based study on the incidence of inflammatory bowel disease in Oviedo (Northern Spain Incidencia de la enfermedad inflamatoria intestinal (EII en población general en el área de Oviedo

    Directory of Open Access Journals (Sweden)

    L. Rodrigo

    2004-05-01

    Full Text Available Objective: to assess the incidence of inflammatory bowel disease in Oviedo (Northern Spain, and to describe the clinical features of new patients. Patients and methods: a prospective population-based study was made at the Health Area IV, Principality of Asturias (Oviedo, 312,324 inhabitants. All new diagnosed patients with inflammatory bowel disease were registered over a 2-year period. Results: a total of 85 patients were included, 47 of these with ulcerative colitis (UC, 37 with Crohn's disease (CD, and 1 with undetermined colitis. The overall adjusted incidence rate of UC and CD per 10(5 inhabitants between 15-64 years was 9.1 (95% CI: 5-13.1 and 7.5 (95% CI: 3.8-11.2, respectively. The global male/female ratio was 0.9, without significant differences between both diseases. CD patients were younger than those with UC (33 ± 15 years vs 45 ± 20 years; p Objetivo: conocer la incidencia de enfermedad inflamatoria intestinal en el área de Oviedo y describir las características clínicas de los nuevos pacientes. Pacientes y métodos: estudio prospectivo poblacional en el Área Sanitaria IV del Principado de Asturias (Oviedo, 312.324 habitantes. Fueron registrados todos los pacientes nuevos diagnosticados de enfermedad inflamatoria intestinal en un periodo de 2 años consecutivos. Resultados: se incluyeron un total de 85 pacientes, 47 con colitis ulcerosa (CU, 37 con enfermedad de Crohn (EC y 1 con colitis indeterminada (CI. La tasa de incidencia ajustada por 10(5 habitantes entre 15 y 64 años, de CU y EC, fue de 9,1 para CU (IC95%: 5-13,1 y 7,5 para la EC (IC95%: 3,8-11,2. La proporción hombre/mujer fue de 0,9, sin diferencias significativas entre ambas enfermedades. Los pacientes con EC eran más jóvenes que los que tenían CU (33 ± 15 años vs 45 ± 20 años; p<0,05. La mayor parte de los pacientes con EC tenían menos de 35 años (65%, mientras que los que presentaban CU fueron diagnosticados entre los 25 y 64 años (81%. La extensi

  1. Intra-Articular Osteoid Osteoma Mimicking Juvenile Arthritis

    Directory of Open Access Journals (Sweden)

    Sidi Yaya Traore

    2014-01-01

    Full Text Available In case of intra-articular osteoid osteoma, misdiagnosis as juvenile arthritis may occur, delaying adequate treatment. We report cases of intra-articular osteoid osteomas in children that were misdiagnosed and initially inappropriately treated with intra-articular corticoid injection. Diagnosis of osteoid osteoma was finally given by CT-scan and appropriate treatment by radiofrequency ablation or surgical ablation was performed. Clinicians and radiologists should be aware of the potentially confusing clinical and imaging findings associated with intra-articular osteoid osteoma.

  2. Asma bronquial. Estado del arte.

    Directory of Open Access Journals (Sweden)

    Jorge Salazar

    2009-10-01

    Full Text Available Para infinidad de personas el asma es su problema de salud. El diagnóstico y el tratamiento varían dependiendo de la magnitud del compromiso y la causa que produce la reacción inflamatoria en los pulmones. La prevención y el tratamiento serán mejores si la población y los pacientes reconocen la necesidad de evitar determinadas exposiciones ambientales y el uso de medicamentos con la frecuencia necesaria. Ahora los médicos generales conocen mejor la fisiología y patología de esta enfermedad y los mecanismos de acción de los diferentes medicamentos. El tratamiento con aerosol debe comprenderse mejor para evitar efectos colaterales en los pacientes y darles una mejor calidad de vida. Se discuten los nuevos tratamientos para el estado asmático y en las unidades de cuidados intensivos.

  3. Papel de la ventilación mecánica durante la circulación extracorporea sobre los mediadores de la respuesta inflamatoria

    OpenAIRE

    López Gomez, Amparo

    2015-01-01

    RESUMEN TESIS: PAPEL DE LA VENTILACIÓN MECÁNICA DURANTE LA CIRCULACIÓN EXTRACORPÓREA SOBRE LOS MEDIADORES DE LA RESPUESTA INFLAMATORIA. La Cardiopatía Isquémica (CI) representa la mayor causa de mortalidad en países desarrollados. En España, supone el mayor número de muertes cardiovasculares, siendo el infarto agudo de miocardio (IMA) la enfermedad isquémica más frecuente. Datos recientes sugieren que se ha producido un aumento de la prevalencia de pacientes con CI . La revasculariza...

  4. Manejo clínico del quiste periapical

    OpenAIRE

    Clara Vergara Hernández

    2013-01-01

    ResumenEl quiste periapical es una lesión inflamatoria de los maxilares formada en el periápice de dientes con pulpa necrótica e infectada siendo una secuela directa de un granuloma apical, están asociados a la raíz dentaria y se encuentran con mayor frecuencia en el maxilar superior. Por lo general no provocan síntomas y su lenta evolución hace que el paciente no se percate del mismo hasta que aparece la asimetría facial. Se presenta un caso correspondiente a un paciente masculino con lesión...

  5. Patología infecciosa: vulvovaginitis, enfermedades de transmisión sexual, enfermedad inflamatoria pélvica, abscesos tubo-ováricos Infectious pathology: vulvovaginitis, sexually transmitted diseases, pelvic inflammatory disease, tubo-ovarian abscesses

    Directory of Open Access Journals (Sweden)

    M. Ibarrola

    2009-01-01

    Full Text Available Las enfermedades de transmisión sexual son aquellas en las que la principal vía de infección es el contacto íntimo. Son numerosas las pacientes que acuden a urgencias por esta causa, tanto por la clínica como por las implicaciones sociales. Los síntomas más frecuentes son dolor abdominal bajo, sangrados vaginales, o flujo vaginal excesivo o molesto. Las vulvovaginitis son uno de los problemas principales en la práctica clínica diaria del ginecólogo. La úlcera genital cuya etiología principal es el herpes, seguida de la sífilis y el chancroide incrementa el riesgo para contraer la infección por el VIH y modifica el curso de otras enfermedades de transmisión sexual. La enfermedad pélvica inflamatoria engloba a las infecciones del tracto genital superior femenino. La importancia del diagnóstico precoz y su tratamiento adecuado reside tanto por las complicaciones en la fase aguda como por las secuelas, que incluyen el dolor crónico y la esterilidad.Sexually transmitted diseases are those where the principal path of infection is through intimate contact. Numerous patients attend Accidents and emergencies for this reason, both because of the clinical features and because of social implications. The most frequent symptoms are lower abdominal pain, vaginal bleeding or excessive or troubling vaginal flow. Vulvovaginites are one of the principal problems in the everyday clinical practice of gynaecology. A genital ulcer whose principal aetiology is herpes, followed by syphilis and chancroid, increases the risk of contracting HIV infection and alters the course of other sexually transmitted diseases. Inflammatory pelvic disease encompasses infections of the upper female genital tract. The importance of early diagnosis and suitable treatment is both due to the complications in its acute phase and to its sequels, which include chronic pain and sterility.

  6. Advances in treatment of articular cartilage injuries

    Directory of Open Access Journals (Sweden)

    Yuan-cheng LI

    2013-05-01

    Full Text Available Cartilage is a kind of terminally differentiated tissue devoid of vessel or nerve, and it is difficult to repair by itself after damage. Many studies for the treatment of cartilage injuries were performed in recent years aiming at repair of the structure and restoration of its function for injured joint. This article reviews the traditional methods of treatment for cartilage injuries, such as joint lavage with the aid of arthroscope, abrasion chondroplasty, laser abrasion and chondroplasty, and drilling of the subchondral bone-marrow space. The research advances in treatment of articular cartilage injuries with tissue engineering were summarized.

  7. Viral articular deformations in a goat

    International Nuclear Information System (INIS)

    A goat belonging to an animal aid association was presented for bilateral deformation of the tarsus and carpus and signs of high-grade pain. ELISA serology was positive for Caprine Arthritis-Encephalitis Virus. Radiography revealed marked osseous remodelling of the tarsus. The blood fibrinogen concentration was very elevated. On infected commercial farms, it is recommended that both seropositive animals and their offspring are culled when the level of infected is low, or to separate the kids from the mothers at an early age. In this case, palliative treatment was prescribed based on non-steroidal anti-inflammatory drugs, antibiotics and articular puncture-lavages

  8. Inestabilidad compleja del codo

    OpenAIRE

    Héctor Gabriel Díaz Carrillo; José A. Meriño Labrada; Nelson Leyva Pérez

    2014-01-01

    La inestabilidad compleja del codo es de reciente descripción y controversial en sus resultados; es el estado que se deriva del daño estructural y funcional, producido por la incongruencia de las superficies articulares y las estructuras ligamentosas que estabilizan el codo. Se realizó un estudio descriptivo prospectivo, en el período comprendido entre enero de 2011 y abril de 2013, en una muestra no probabilística de ocho pacientes (diez codos), seleccionados según criterios de inclusión y e...

  9. Ozonoterapia sistémica e intra-articular en la artritis de la articulación temporomandibular por artritis reumatoide

    Directory of Open Access Journals (Sweden)

    Ivonne Méndez-Pérez

    2010-01-01

    Full Text Available Se estudió el efecto de la ozonoterapia intra-articular combinada con la vía sistémica (vía rectal y se comparó con la sola aplicación por vía intra-articular en pacientes con artritis de la articulación temporomandibular (ATM por artritis reumatoide (AR mediante la evaluación clínica del dolor a la masticación, chasquido y trismo. Fueron estudiados veinte pacientes, divididos en dos grupos de diez cada uno, el primero recibió ozonoterapia intra-articular (concentración de ozono 10 mg/L y volumen de 3 mL, a razón de dos aplicaciones por semana, durante cinco semanas, y el segundo, terapia combinada (igual al primer grupo más ozono administrado por insuflación rectal, a una concentración de 30 hasta 40 mg/L y un volumen de 100 hasta 200 mL, veinte aplicaciones. Se evaluó el grosor del cartílago articular (por ultrasonido, al inicio y al final del tratamiento al igual que los síntomas clínicos: dolor a la masticación, chasquido y trismo. Como resultado, se obtuvo una disminución significativa de todos los síntomas estudiados, así como del grosor del cartílago articular diagnosticado en el estudio ultrasonográfico, teniendo una respuesta más rápida en el grupo de la terapia combinada. Se concluyó que ambas formas de tratamiento demuestran efectos beneficiosos en la artritis de la articulación temporomandibular de pacientes con artritis reumatoide, aunque la terapia combinada resulta la más eficiente, resultado que no ha sido reportado con anterioridad.

  10. Epidemiology of chronic inflammatory bowel disease in the Northern area of Huelva Estudio epidemiológico de la enfermedad inflamatoria intestinal en la zona norte de Huelva

    Directory of Open Access Journals (Sweden)

    A. Garrido

    2004-10-01

    Full Text Available Objective: to know the different epidemiologic aspects of chronic inflammatory bowel disease (IBD in the Northern area of the province of Huelva. Material and methods: we carried out a retrospective (1980-1996 and prospective (1996-2003 study of all patients diagnosed with IBD in the Northern area of Huelva, with 77,856 inhabitants. The distribution of Crohn's disease (CD and ulcerative colitis (UC was analyzed, as well as sex, age and smoking habit at the time of diagnosis, familial aggregation, appendicectomy rate, phenotype (anatomical site and clinical types, extraintestinal manifestations, and immunosuppressive therapy or surgical requirements. Results: 70 patients with IBD were studied, 40 with UC and 30 with CD. Sex distribution was 39 (55.7% males (55% with CD vs 56.6% with UC; NS and 31 (44.3% females (45% with CD vs 43.3% with UC; NS. Mean age at presentation was 44.7 ± 19.32 years in UC vs 32.3 ± 16.43 in CD; p Objetivo: conocer los distintos aspectos epidemiológicos de la enfermedad inflamatoria intestinal (EII en la zona norte de la provincia de Huelva. Material y métodos: se realizó un estudio retrospectivo (1980-1996 y prospectivo (1996-2003 sobre los pacientes diagnosticados de EII en la zona norte de Huelva con 77.856 habitantes. Se ha analizado la distribución de enfermedad de Crohn (EC y colitis ulcerosa (CU, sexo, edad y hábito tabáquico en el momento del diagnóstico, agregación familiar, antecedentes de apendicetomía, fenotipo (extensión y tipos clínicos, manifestaciones extraintestinales y necesidad de tratamiento inmunosupresor y/o quirúrgico. Resultados: se estudiaron 70 pacientes con EII, 40 con CU y 30 con EC. Un total de 39 (55,7% eran varones (55% en EC frente a 56,6% en CU; NS y 31 (44,3% mujeres (45% en EC frente a 43,3% en CU; NS. La media de edad en el momento de diagnóstico fue de 44,7±19,32 para CU frente a 32,3±16,43 para EC (p<0,001. La asociación familiar fue del 7,1%. Referían apendicectom

  11. Toward patient-specific articular contact mechanics.

    Science.gov (United States)

    Ateshian, Gerard A; Henak, Corinne R; Weiss, Jeffrey A

    2015-03-18

    The mechanics of contacting cartilage layers is fundamentally important to understanding the development, homeostasis and pathology of diarthrodial joints. Because of the highly nonlinear nature of both the materials and the contact problem itself, numerical methods such as the finite element method are typically incorporated to obtain solutions. Over the course of five decades, we have moved from an initial qualitative understanding of articular cartilage material behavior to the ability to perform complex, three-dimensional contact analysis, including multiphasic material representations. This history includes the development of analytical and computational contact analysis methods that now provide the ability to perform highly nonlinear analyses. Numerical implementations of contact analysis based on the finite element method are rapidly advancing and will soon enable patient-specific analysis of joint contact mechanics using models based on medical image data. In addition to contact stress on the articular surfaces, these techniques can predict variations in strain and strain through the cartilage layers, providing the basis to predict damage and failure. This opens up exciting areas for future research and application to patient-specific diagnosis and treatment planning applied to a variety of pathologies that affect joint function and cartilage homeostasis.

  12. PRP and Articular Cartilage: A Clinical Update

    Science.gov (United States)

    Rossi, Roberto; Castoldi, Filippo; Michielon, Gianni

    2015-01-01

    The convincing background of the recent studies, investigating the different potentials of platelet-rich plasma, offers the clinician an appealing alternative for the treatment of cartilage lesions and osteoarthritis. Recent evidences in literature have shown that PRP may be helpful both as an adjuvant for surgical treatment of cartilage defects and as a therapeutic tool by intra-articular injection in patients affected by osteoarthritis. In this review, the authors introduce the trophic and anti-inflammatory properties of PRP and the different products of the available platelet concentrates. Then, in a complex scenario made of a great number of clinical variables, they resume the current literature on the PRP applications in cartilage surgery as well as the use of intra-articular PRP injections for the conservative treatment of cartilage degenerative lesions and osteoarthritis in humans, available as both case series and comparative studies. The result of this review confirms the fascinating biological role of PRP, although many aspects yet remain to be clarified and the use of PRP in a clinical setting has to be considered still exploratory. PMID:26075244

  13. [Articular chondrocalcinosis after 80 years of age].

    Science.gov (United States)

    Memin, Y; Monville, C; Ryckewaert, A

    1978-02-01

    In 108 women over 80 (mean age 88,4 years, extremes 80 and 99 years) hospitalized in a geriatric service for various reasons, radiograms were made of both knees in the frontal aspect on standard film to detect calcinosis of the meniscus and chondrocalcinosis of the joint. In 25 women (23.1%) the radiographs revealed calcinosis of the meniscus with or without chondrocalcinosis. In these 25 cases a lateral X-ray was also made of the two knees, frontal X-rays were made of the pelvis, thumbs and shoulders. In 22 cases (88%) these revealed calcification of the fibrocartilages or articular cartilages in joints other than the knee. Seven of the 25 women had at least one attack of articular inflammation (especially of the knee) resembling a pseudo-gout crisis. The frequency of chronic arthropathies resembling arthroses was high in the 25 patients with chondrocalcinosis: 8 (32%) had an internal or external femoro-tibial arthrosis, as against 11 of the 83 patients (13%) of the same age without chondrocalcinosis, a significant difference. Eleven of the 25 women had signs of femororotular arthrosis on the lateral X-rays of the knees, 5 had coxarthrosis (with in 3 cases a radiological image of fibrocartilaginous or coxofemoral cartilaginous calcification). One women had chronic radiocarpal arthropathy evocative or chondrocalcinosis. Ten had a scaphotrapezoidal arthrosis, 5 arthrosis of the shoulder, 3 with radiological aspect of glenohumeral chondrocalcinosis. PMID:644241

  14. Modelos animales para el estudio de la respuesta inflamatoria sistémica y de nutrición parenteral Animal models for the study of systemic inflammatory response and parenteral nutrition

    Directory of Open Access Journals (Sweden)

    J. M. Morán Penco

    2007-04-01

    Full Text Available El Síndrome de Respuesta Inflamatoria Sistémica (SRIS parece desencadenarse por la activación de un tipo de receptores llamados Toll-Like, propios de las células de respuesta inflamatoria y que, a través de señales citosólicas específicas, producen una cascada de reacciones que activan las Citocinas, Factores de Crecimiento y otros mediadores inflamatorios. En este trabajo, repasamos y discutimos varias clasificaciones de Modelos Animales para el estudio del SRIS y proponemos que estos modelos se dividan según los objetivos concretos a estudiar; que podrían ser los siguientes: 1º Para el estudio de los genes reguladores de los receptores Innatos y Adaptativos, de la respuesta inmunoinflamatoria. 2º Para el estudio de los receptores de señalización (Citocinas y Factores de Crecimiento. 3º Para el estudio de la respuesta frente a los señalizadores. 4º Para el estudio de tratamientos mediante bloqueo anti-inflamatorio específico (ILs, TNF y otros. 5º Modelos específicos de Sepsis. 6º Otros Modelos Inductores de SRIS. 7º Para el estudio de Modelos Terapéuticos diversos: -Tratamientos Anti-Inflamatorios. -Tratamientos Anticoagulantes: Inhibición de la Coagulación en ensayos Humanos-Fase II, con anticoagulantes: Antitrombina III, PCA y TFPI. -Tratamientos Antibióticos. -Tratamientos con Reposición de Volúmenes. -Tratamientos Quirúrgicos. En lo que respecta al apartado de Modelos animales para el estudio de la Nutrición Parenteral, podríamos hacer la siguiente clasificación y resumen de lo tratado: 1. Modelos Animales para el estudio de la Vía Parenteral de Administración. 2. Modelos para el estudio de la viabilidad, absorción y tolerancia locales en la vía de administración. 3. Modelos para el estudio de las Complicaciones. 4. Modelos animales para el estudio de la farmacodinamia, metabolización e investigación de la tolerancia de nuevas moléculas o substratos.The systemic inflammatory response syndrome (SRIS

  15. Biologic therapies for chronic inflammatory bowel disease Tratamientos biológicos en la enfermedad inflamatoria crónica intestinal

    Directory of Open Access Journals (Sweden)

    M. P. Martínez-Montiel

    2006-04-01

    Full Text Available Crohn's disease (CD and ulcerative colitis (UC make up the so-called chronic inflammatory bowel disease (IBD. Advances in the understanding of IBD pathophysiologic mechanisms in the last few years have allowed the development of novel therapies such as biologic therapies, which at least theoretically represent a more specific management of this disease with fewer side effects. Currently, the only effective and widely accepted biologic therapy for the treatment of intraluminal, fistulizing CD, both for remission induction and maintenance, is infliximab. The role of other monoclonal antibodies such as adalimumab is not clearly established. It could be deemed an alternative for patients with allergic reactions to infliximab, and for those with lost response because of anti-infliximab antibody development. However, relevant issues such as dosage and administration regimen remain to be established. Anti-integrin α4 therapies, despite encouraging results in phase-3 studies, are still unavailable, as their marketing authorization was held back in view of a number of reports regarding progressive multifocal leukoencephalopathy cases. Immunostimulating therapy may be highly relevant in the near future, as it represents a novel strategy against disease with the inclusion of granulocyte-monocyte colony-stimulating factors. Regarding ulcerative colitis, results from the ACT-1 and ACT-2 studies showed that infliximab is also useful for the management of serious UC flare-ups not responding to standard treatment, which will lead to a revision of therapeutic algorithms, where this drug should be given preference before intravenous cyclosporine. In the next few years, the role of anti-CD3 drugs (vilisilizumab, T-cell inhibiting therapies, and epithelial repair and healing stimulating factors will be established.La enfermedad de Crohn (EC y la colitis ulcerosa (CU constituyen la denominada enfermedad inflamatoria crónica intestinal (EII. Los avances producidos

  16. Fibroma aponeurótico calcificante intra articular. Presentación de un caso.

    Directory of Open Access Journals (Sweden)

    Niurka Rabelo Aday

    2010-02-01

    Full Text Available El fibroma aponeurótico juvenil es un tumor infrecuente del tejido blando que se localiza con mayor periodicidad en niños y adolescentes en la región distal de las extremidades, aunque se reportan casos en otras edades y localización. Se presenta un paciente de 18 años de edad, masculino con fibroma aponeurótico calcificante en la rodilla derecha intra articular, con una localización poco común. El diagnóstico se realizó por exéresis quirúrgica del tumor. Se hace referencia al cuadro clínico, terapéutica efectuada y seguimiento por 3 años. Se revisó literatura actualizada referente al tema. Por ser un caso infrecuente se decide su publicación.

  17. Effects of intra articular tramadol on articular cartilage and synovium of rats

    Directory of Open Access Journals (Sweden)

    Musa Kola

    2015-12-01

    Full Text Available Objective: To investigate the effects of intra articular tramadol injection on articular cartilage and synovium in rat knee joint. Methods: After Animal Ethical Committee approval, a total of 20 Sprague-Dawley rats were used and divided into 4 groups. Each group was composed of 5 rats. 0.2 ml of tramadol HCl was injected into the right knee joints and left knee joints of all the rats were considered as control. Control side joints received saline injection. Rats were sacrificed with ketamin on 1st, 7th, 14th and 21st days and knee joints were removed. Obtained tissue samples were decalcified and were dyed with Hematoxylin-eosin and Masson’s trichrome stain and examined by light microscopy for the presence of inflammation in periarticular area and synovia. Results: Congestion of synovial veins and perivascular cell infiltration were observed in tramadol group on days 1 and 7 (P < 0.05. The inflammation process was replaced by fibrosis on day 14 and fibrosis was significantly decreased on day 21. Conclusion: Intra articular tramadol should be used cautiously, keeping in mind that it may cause synovial inflammation in early phases and fibrosis in late phases. J Clin Exp Invest 2015; 6 (4: 337-342

  18. Relationship between socio-demographic and clinical variables, and health-related quality of life in patients with inflammatory bowel disease Relación entre variables sociodemográficas y clínicas y calidad de vida relacionada con la salud en pacientes con enfermedad inflamatoria intestinal

    Directory of Open Access Journals (Sweden)

    B. López Blanco

    2005-12-01

    Full Text Available Objective: the principal aim of the present study is to evaluate the influence of socio-demographic and clinical variables on health-related quality of life (HRQL in patients with inflammatory bowel disease. Patients and method: this was a cross-sectional study. health-related quality of life was measured with the Inflammatory Bowel Disease Questionnaire (IBDQ. A total of 120 patients, 60 with ulcerative colitis and 60 with Crohn's disease, participated in the study. Results: no significant differences were observed between ulcerative colitis and Crohn's disease patients in IBDQ dimensions. However, a multivariate analysis revealed that sex, type of treatment, extraintestinal symptoms, number of relapses in previous year, satisfaction with surgery, and need for psychological support were related to HRQL. Conclusions: the identification of these variables associated with HRQL in patients with inflammatory bowel disease shows them to be basically non-disease factors. Knowledge of such elements can turn out to be very useful in order to guide future research and modify specific factors in further interventions.Objetivo: el objetivo principal del presente trabajo consiste en evaluar la influencia de las variables sociodemográficas y clínicas en la calidad de vida relacionada con la salud de los pacientes con enfermedad inflamatoria intestinal. Pacientes y método: se trata de un estudio transversal. La calidad de vida relacionada con la salud se investigó utilizando el "Inflammatory Bowel Disease Questionnaire" (IBDQ. Un total de 120 pacientes, 60 con colitis ulcerosa y 60 con enfermedad de Crohn participaron en el estudio. Resultados: no se apreciaron diferencias significativas en la calidad de vida relacionada con la salud en las dimensiones del IBDQ en función de la enfermedad, sin embargo, tras el análisis multivariante, el sexo, el tipo de tratamiento, las manifestaciones extradigestivas, el número de recaídas anual, la satisfacción con

  19. Evolución del germen dental trasplantado heterotópicamente en la dermis: un estudio histológico en la rata

    OpenAIRE

    Mezadri, Telmo José; Rivero Tames, David; Boabaid, Fernanda; Armengol, José A.

    2004-01-01

    En este estudio hemos analizado la potencialidad de desarrollo del germen dental en fase de corona. Para ello, los gérmenes dentales de rata recién nacida fueron trasplantados en las bolsas dérmicas de la oreja de ratas isogénicas. Siete y catorce días después del trasplante, se desarrollaron las estructuras morfológicas dentales típicas, con ameloblastos y odontoblastos bien diferenciados. Ulteriormente, los procesos de reacción inflamatoria del tejido huésped con infilt...

  20. Chronic Inflammatory Periodontal Disease in Patients with Human Immunodeficiency Virus. Enfermedad periodontal inflamatoria crónica en pacientes infectados con el virus de inmunodeficiencia humana.

    Directory of Open Access Journals (Sweden)

    Iralys Benítez Guzmán

    2009-07-01

    Full Text Available Background: The Chronic Inflammatory Periodontal Disease is related with multiple risk factors. Those patients with human immunodeficiency virus have higher risk of presenting this disease and it is usually more serious in these cases. Objective: To describe the prevalence of Chronic Inflammatory Periodontal Disease in patients with HIV. Methods: Descriptive, observational, cross-sectional study including patients with HIV in Sancti Spiritus province. The occurrence of the disease was determined after the Periodontics Cuban Standards, and oral hygiene was assessed through the simplified oral hygiene index. Other variables were measured, such as smoking habits, T CD4+ lymphocyte counting and virus load. The independent association of each risk factor with the disease was determined through a logistic regression model. Results: The 56, 5 % of the 154 patients presented Chronic Inflammatory Periodontal Disease; 60 (39.0% gingivitis and 27 (17,5% periodontitis. Gingivitis was associated with poor oral hygiene (OR: 3,71 and periodontitis with smoking habit (OR: 5,20. The severe forms of periodontitis occurred mainly in patients with lymphocyte counting lower than 500 cells/mm3 . Conclusions: The prevalence of Chronic Inflammatory Periodontal Disease in patients with HIV in Sancti Spiritus province is linked to known risk factors such as smoking habits and oral hygiene.Fundamento: La enfermedad periodontal inflamatoria crónica es un trastorno relacionado con diversidad de factores de riesgo. Los pacientes infectados con el virus de inmunodeficiencia humana tienen mayor riesgo para padecerla y en ellos muchas veces se agrava.  Objetivo: Describir la prevalencia de la enfermedad periodontal inflamatoria crónica en pacientes infectados con el virus de inmunodeficiencia humana. Métodos: Estudio observacional

  1. Intra-articular ganglion cysts of the cruciate ligaments

    Energy Technology Data Exchange (ETDEWEB)

    Tyrrell, P.N.M.; Cassar-Pullicino, V.N.; McCall, I.W. [Department of Diagnostic Imaging and The Institute of Orthopaedics, The Robert Jones and Agnes Hunt Orthopaedic and District Hospital, Oswestry, Shropshire (United Kingdom)

    2000-08-01

    Intra-articular ganglion cysts of the cruciate ligaments are associated with non-specific clinical signs and symptoms. Familiarity with the MR appearances in particular is important to make an accurate diagnosis, exclude associated abnormalities, and avoid misdiagnosis. (orig.)

  2. [Structure of the articular cartilage in the middle aged].

    Science.gov (United States)

    Kop'eva, T N; Mul'diiarov, P Ia; Bel'skaia, O B; Pastel', V B

    1983-10-01

    In persons 17-83 years of age having no articular disorders 39 samples of the patellar articular cartilage, the articulated surface and the femoral head have been studied histochemically, histometrically and electron microscopically. Age involution of the articular cartilage is revealed after 40 years of age as a progressive decrease in chondrocytes density in the superficial and (to a less degree) in the intermediate zones. This is accompanied with a decreasing number of 3- and 4-cellular lacunae and with an increasing number of unicellular and hollow lacunae. In some chondrocytes certain distrophic and necrotic changes are revealed. In the articular matrix the zone with the minimal content of glycosaminoglycans becomes thicker and keratansulfate content in the territorial matrix of the cartilage deep zone grows large.

  3. Insight into osteo-articular digital tomosynthesis: a pictorial essay

    International Nuclear Information System (INIS)

    The objectives of this pictorial essay are to describe osteo-articular imaging using digital tomosynthesis. We provide characteristic examples in eight patients. Digital tomosynthesis allows easy, fast and low-dose imaging, with excellent spatial resolution.

  4. Ultrasound Backscattering Is Anisotropic in Bovine Articular Cartilage.

    Science.gov (United States)

    Inkinen, Satu I; Liukkonen, Jukka; Tiitu, Virpi; Virén, Tuomas; Jurvelin, Jukka S; Töyräs, Juha

    2015-07-01

    Collagen, proteoglycans and chondrocytes can contribute to ultrasound scattering in articular cartilage. However, anisotropy of ultrasound scattering in cartilage is not fully characterized. We investigate this using a clinical intravascular ultrasound device with ultrasound frequencies of 9 and 40 MHz. Osteochondral samples were obtained from intact bovine patellas, and cartilage was imaged in two perpendicular directions: through articular and lateral surfaces. At both frequencies, ultrasound backscattering was higher (p < 0.05) when measured through the lateral surface of cartilage. In addition, the composition and structure of articular cartilage were investigated with multiple reference methods involving light microscopy, digital densitometry, polarized light microscopy and Fourier infrared imaging. Reference methods indicated that acoustic anisotropy of ultrasound scattering arises mainly from non-uniform distribution of chondrocytes and anisotropic orientation of collagen fibers. To conclude, ultrasound backscattering in articular cartilage was found to be anisotropic and dependent on the frequency in use. PMID:25933711

  5. Estudo comparativo da analgesia entre bupivacaína e morfina intra-articular em osteoartrite de joelho Estudio comparativo de la analgesia entre bupivacaína y morfina intra-articular en osteoartritis de la rodilla Intra-articular bupivacaine and morphine for knee osteoarthritis analgesia. Comparative study

    Directory of Open Access Journals (Sweden)

    Miriam C B Gazi

    2005-10-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A osteoartrite é a mais freqüente entre as doenças articulares em pessoas idosas. O objetivo do estudo foi comparar o efeito analgésico da bupivacaína e da morfina por via intra-articular em pacientes portadores de osteoartrite de joelho. MÉTODO: Foram avaliados 39 pacientes em estudo duplamente encoberto, divididos de forma aleatória, em dois grupos: os do G1 (n = 18 receberam 1 mg (1 mL de morfina diluída em 9 mL de solução fisiológica a 0,9% e os do G2 (n = 21, 25 mg (10 mL de bupivacaína a 0,25% sem vasoconstritor, por via intra-articular. A intensidade da dor foi avaliada pela escala numérica e verbal nos momentos 0, 30, 60 minutos e 7 dias, em repouso e em movimento. Foram avaliados a necessidade de complementação analgésica com paracetamol (500 mg, a dose total de analgésico utilizado, a duração da analgesia e a qualidade da analgesia (pelo paciente. RESULTADOS: Dos 39 pacientes, 31 completaram o estudo. Não houve diferença significativa da intensidade da dor em repouso e em movimento entre os dois grupos nos momentos estudados. Não houve diferença entre os dois grupos no tempo entre a administração da solução e a necessidade de complementação analgésica. A dose média do paracetamol utilizada no primeiro dia da semana foi de 796 mg do G1 e de 950 mg no G2; a complementação na semana foi de 3578 mg no G1 e 5333 mg no G2. CONCLUSÕES: O efeito analgésico de 1 mg de morfina e de 25 mg de bupivacaína a 0,25% sem vasoconstritor intra-articular foram semelhantes.JUSTIFICATIVA Y OBJETIVOS: La osteoartritis es la más frecuente entre las enfermedades articulares en personas de edad. El objetivo del estudio fue comparar el efecto analgésico de la bupivacaína y de la morfina por vía intra-articular en pacientes portadores de osteoartritis de rodilla. MÉTODO: Fueron evaluados 39 pacientes en estudio doblemente encubierto, divididos de forma aleatoria, en dos grupos: los del G1 (n = 18

  6. The Frictional Coefficient of Bovine Knee Articular Cartilage

    Institute of Scientific and Technical Information of China (English)

    Qian Shan-hua; Ge Shi-rong; Wang Qing-liang

    2006-01-01

    The normal displacement of articular cartilage was measured under load and in sliding, and the coefficient of friction during sliding was measured using a UMT-2 Multi-Specimen Test System. The maximum normal displacement under load and the start-up frictional coefficient have similar tendency of variation with loading time. The sliding speed does not significantly influence the frictional coefficient of articular cartilage.

  7. Type III Collagen, a Fibril Network Modifier in Articular Cartilage*

    OpenAIRE

    Wu, Jiann-Jiu; Weis, Mary Ann; Kim, Lammy S.; Eyre, David R.

    2010-01-01

    The collagen framework of hyaline cartilages, including articular cartilage, consists largely of type II collagen that matures from a cross-linked heteropolymeric fibril template of types II, IX, and XI collagens. In the articular cartilages of adult joints, type III collagen makes an appearance in varying amounts superimposed on the original collagen fibril network. In a study to understand better the structural role of type III collagen in cartilage, we find that type III collagen molecules...

  8. Pendulum mass affects the measurement of articular friction coefficient.

    Science.gov (United States)

    Akelman, Matthew R; Teeple, Erin; Machan, Jason T; Crisco, Joseph J; Jay, Gregory D; Fleming, Braden C

    2013-02-01

    Friction measurements of articular cartilage are important to determine the relative tribologic contributions made by synovial fluid or cartilage, and to assess the efficacy of therapies for preventing the development of post-traumatic osteoarthritis. Stanton's equation is the most frequently used formula for estimating the whole joint friction coefficient (μ) of an articular pendulum, and assumes pendulum energy loss through a mass-independent mechanism. This study examines if articular pendulum energy loss is indeed mass independent, and compares Stanton's model to an alternative model, which incorporates viscous damping, for calculating μ. Ten loads (25-100% body weight) were applied in a random order to an articular pendulum using the knees of adult male Hartley guinea pigs (n=4) as the fulcrum. Motion of the decaying pendulum was recorded and μ was estimated using two models: Stanton's equation, and an exponential decay function incorporating a viscous damping coefficient. μ estimates decreased as mass increased for both models. Exponential decay model fit error values were 82% less than the Stanton model. These results indicate that μ decreases with increasing mass, and that an exponential decay model provides a better fit for articular pendulum data at all mass values. In conclusion, inter-study comparisons of articular pendulum μ values should not be made without recognizing the loads used, as μ values are mass dependent. PMID:23122223

  9. Nutrición artificial en la insuficiencia intestinal: síndrome de intestino corto: Enfermedad inflamatoria intestinal Artificial nutrition in intestinal failure: short bowel syndrome. Intestinal inflammatory disease

    Directory of Open Access Journals (Sweden)

    T. Grau Carmona

    2005-06-01

    Full Text Available La resección intestinal amplia produce suficientes alteraciones como para requerir soporte nutricional especializado. Las medidas básicas de tratamiento, especial-mente en la fase aguda tras la resección intestinal o en presencia de complicaciones graves sobre pacientes con intestino corto, incluyen la repleción de fluidos y electro-litos y la instauración de soporte nutricional con el fin de prevenir la malnutrición. La nutrición enteral es el principal factor estimulador de la adaptación del intestino remanente. No obstante, su aplicación presenta dificultades en las fases agudas, por lo que los pacientes deben ser tratados frecuentemente con nutrición parenteral. La presencia de desnutrición puede ser también de importancia en los pacientes con enfermedad inflamatoria intestinal. El soporte nutricional está indicado en estos casos como tratamiento primario de la enfermedad, como tratamiento de la desnutrición o como tratamiento perioperatorio en los pacientes que requieren cirugía. A pesar de la patología digestiva, existen datos para recomendar la nutrición enteral como método inicial para el aporte de nutrientes en los pacientes que lo precisen.Large intestinal resection produces a sufficient number of impairments as to require specialized nutritional support. Basic treatment measures, especially during the acute phase after intestinal resection or in the presence of severe complications in patients with short bowel syndrome, include fluid and electrolytes reposition and nutritional support implementation in order to prevent hyponutrition. Enteral nutrition is the main stimulating factor for adaptation of the remaining bowel. However, its application has some difficulties during the acute phase, and thus patients must be frequently treated with parenteral nutrition. The presence of hyponutrition may be also important in patients with intestinal inflammatory disease. Nutritional support is indicated in these cases as the

  10. Ángulo óptimo articular y ejercicio: bases y aplicaciones

    Directory of Open Access Journals (Sweden)

    Pablo Antonio Rubio-Sobrino

    2012-09-01

    Full Text Available El ángulo óptimo se refiere a la posición articular en la que se produce el pico máximo de momento de fuerza, dentro de la curva ángulo-momento articular. Este parámetro puede utilizarse para estimar las modificaciones en la curva fuerza-longitud de un grupo muscular. El ángulo óptimo puede ser obtenido a partir de medidas en tests isométricos o dinámicos, y esto debe ser tenido en cuenta a la hora de establecer comparaciones. El ángulo óptimo puede ser modificado tanto de forma aguda como crónica. Los principales factores que lo modifican son la intensidad y el volumen del ejercicio, la longitud muscular, el modo de contracción y la velocidad de contracción. La capacidad para evaluar y modificar el ángulo óptimo puede utilizarse en programas de prevención de lesiones musculares, especialmente en deportes de equipo como el fútbol. Esta revisión analiza y discute los estudios que se encuentran en la bibliografía relacionados con este tema.

  11. Intra-articular injection of Torin 1 reduces degeneration of articular cartilage in a rabbit osteoarthritis model

    Science.gov (United States)

    Cheng, N-T.; Cui, Y-P.

    2016-01-01

    Objectives Recent studies have shown that systemic injection of rapamycin can prevent the development of osteoarthritis (OA)-like changes in human chondrocytes and reduce the severity of experimental OA. However, the systemic injection of rapamycin leads to many side effects. The purpose of this study was to determine the effects of intra-articular injection of Torin 1, which as a specific inhibitor of mTOR which can cause induction of autophagy, is similar to rapamycin, on articular cartilage degeneration in a rabbit osteoarthritis model and to investigate the mechanism of Torin 1’s effects on experimental OA. Methods Collagenase (type II) was injected twice into both knees of three-month-old rabbits to induce OA, combined with two intra–articular injections of Torin 1 (400 nM). Degeneration of articular cartilage was evaluated by histology using the Mankin scoring system at eight weeks after injection. Chondrocyte degeneration and autophagosomes were observed by transmission electron microscopy. Matrix metallopeptidase-13 (MMP-13) and vascular endothelial growth factor (VEGF) expression were analysed by quantitative RT-PCR (qPCR).Beclin-1 and light chain 3 (LC3) expression were examined by Western blotting. Results Intra-articular injection of Torin 1 significantly reduced degeneration of the articular cartilage after induction of OA. Autophagosomes andBeclin-1 and LC3 expression were increased in the chondrocytes from Torin 1-treated rabbits. Torin 1 treatment also reduced MMP-13 and VEGF expression at eight weeks after collagenase injection. Conclusion Our results demonstrate that intra-articular injection of Torin 1 reduces degeneration of articular cartilage in collagenase-induced OA, at least partially by autophagy activation, suggesting a novel therapeutic approach for preventing cartilage degeneration and treating OA. Cite this article: N-T. Cheng, A. Guo, Y-P. Cui. Intra-articular injection of Torin 1 reduces degeneration of articular cartilage in a

  12. Efecto antiinflamatorio y antioxidante del extracto hidroalcohólico de Petiveria alliacea

    OpenAIRE

    César Zaa; Martha Valdivia; Álvaro Marcelo

    2013-01-01

    Los radicales libres y el daño oxidativo están relacionados con la muerte celular, siendo la peroxidación lipídica un mecanismo que lleva a la destrucción oxidativa de la membrana celular. Además, en procesos inflamatorios, uno de los primeros estadios es la permeabilidad vascular incrementada (formación del edema) seguido de la extravasación de los fluídos, migración de leucocitos al sitio dañado y liberación de proteínas pro-inflamatorias. Evaluamos el efecto antioxidante y antiinflamat...

  13. EFECTO DE UN PROGRAMA DE HIDROQUINESIA Y ACONDICIONAMIENTO FÍSICO EN LA MOVILIDAD ARTICULAR DE ADULTAS MAYORES

    OpenAIRE

    Cinthya Campos Salazar; Luis Solano Mora

    2001-01-01

    El propósito de este estudio fue determinar el efecto de un programa combinado de hidroquinesia y acondicionamiento físico y otro de acondicionamiento físico solamente en la movilidad articular en las caderas de un grupo de adultas mayores. Sesenta y tres sujetos (edad promedio = 66.06 años, S.D. ±6.77) fueron asignados a los grupos control, hidroterapia y acondicionamiento físico y solo acondicionamiento físico. La duración del programa fue de una hora por sesión, dos veces a la semana duran...

  14. Modulación del receptor nicotínico por moléculas de relevancia clínica

    OpenAIRE

    Alberola Die, Armando; Soriano Úbeda, Sergi; Ivorra Pastor, Isabel; Morales Calderón, Andrés

    2013-01-01

    El receptor nicotínico de acetilcolina (nAChR) es el miembro mejor caracterizado de la superfamilia de canales iónicos activados por ligando (LGICs). Las disfunciones del nAChR se han relacionado con graves alteraciones fisiopatológicas del sistema nervioso, así como con alteraciones inflamatorias e inmunológicas y con el desarrollo de ciertos tipos de cáncer. Por ello, el conocimiento de las moléculas que modulan la función de este receptor, el estudio de sus mecanismos de acción y la diluci...

  15. Manejo clínico odontológico integral del paciente con síndrome de Sjögren: una propuesta

    OpenAIRE

    G. Sturla Rojas; F Romo Ormazábal; M.A. Torres-Quintana

    2014-01-01

    El síndrome de Sjögren (SS) es una enfermedad autoinmune, crónica e inflamatoria caracterizada por infiltración de células plasmáticas y linfocitos en las glándulas exocrinas, particularmente en las salivales y oculares. La patogénesis del SS está relacionada con factores inmunológicos, neurológicos, genéticos, virales y hormonales. La deficiente calidad y cantidad de saliva trae consecuencias devastadoras para la salud dental y bucal, alteraciones del esmalte, caries en las superficies denta...

  16. REGENERATION OF ARTICULAR CARTILAGE UNDER THE IMPLANTATION OF BONE MATRIX

    Directory of Open Access Journals (Sweden)

    Yuri M. Iryanov, Nikolay A. Kiryanov, Olga V. Dyuriagina , Tatiana Yu. Karaseva, Evgenii A. Karasev

    2015-07-01

    Full Text Available Background: The damage or loss of articular cartilage is costly medical problem. The purpose of this work – morphological analysis of reparative chondrogenesis when implanted in the area of the knee joint cartilage of granulated mineralized bone matrix. Material and Methods: The characteristic features of the knee cartilage regeneration studied experimentally in pubertal Wistar rats after modeling a marginal perforated defect and implantation of granulated mineralized bone matrix obtained according to original technology without heat and demineralizing processing into the injury zone. Results: This biomaterial established to have pronounced chondro- and osteoinductive properties, and to provide prolonged activation of reparative process, accelerated organotypical remodeling and restoration of the articular cartilage injured. Conclusion: The data obtained demonstrate the efficacy of МВМ in clinical practice for the treatment of diseases and injuries of the articular cartilage.

  17. Rotura aguda completa del ligamento colateral metacarpo-falángico radial del pulgar: una lesión grave infrecuente

    OpenAIRE

    Pellicer García, V.; Gutiérrez Carbonell, Pedro; Moril Peñalver, L.

    2012-01-01

    El ligamento colateral radial es el principal estabilizador articular contra las fuerzas de estrés en aducción de la articulación metacarpofalángica del pulgar. Esta lesión infrecuente, pasa a menudo desapercibida, desarrollándose una inestabilidad articular sintomática y, posteriormente, una artrosis secundaria. Aunque tradicionalmente se ha recomendado el tratamiento conservador de estas lesiones, estudios recientes han documentado mejores resultados funcionales cuando se realiza su repa...

  18. Changes in articular cartilage after meniscectomy and meniscus replacement using a biodegradable porous polymer implant

    NARCIS (Netherlands)

    Hannink, G.J.; Tienen, T.G. van; Schouten, A.J.; Buma, P.

    2011-01-01

    PURPOSE: To evaluate the long-term effects of implantation of a biodegradable polymer meniscus implant on articular cartilage degeneration and compare this to articular cartilage degeneration after meniscectomy. METHODS: Porous polymer polycaprolacton-based polyurethane meniscus implants were implan

  19. Intra-Articular Osteotomy for Distal Humerus Malunion

    Directory of Open Access Journals (Sweden)

    René K. Marti

    2009-01-01

    Full Text Available Intra-articular osteotomy is considered in the rare case of malunion after a fracture of the distal humerus to restore humeral alignment and gain a functional arc of elbow motion. Traumatic and iatrogenic disruption of the limited blood flow to the distal end of the humerus resulting in avascular necrosis of capitellum or trochlea is a major pitfall of the this technically challenging procedure. Two cases are presented which illustrate the potential problems of intra-articular osteotomy for malunion of the distal humerus.

  20. Caracterización de las acciones protectoras del ácido oleanólico en modelos experimentales de patologías con base inflamatoria

    OpenAIRE

    Pereira Córdova Marcos, Cláudia Manuela

    2015-01-01

    La Esclerosis Múltiple y la Miocarditis son dos enfermedades autoinmunes que se deben a la alteración en la respuesta inmune donde el organismo no reconoce el propio de lo extraño. La Conjuntivitis Alérgica se debe a alteraciones de la respuesta inmune asociada a reacciones de hipersensibilidad y es una respuesta inmune exagerada a un agente inocuo. El ácido oleanólico (AO) es un compuesto natural que se encuentra en numerosas plantas entre las cuales el olivo y presenta propiedades: cardiopr...

  1. Parasitosis intestinal extrema en la infancia previa al desarrollo de enfermedades reumáticas inflamatorias/autoinmunes

    OpenAIRE

    F. Javier Jiménez-Balderas; Adolfo Camargo-Coronel; Juan O. Talavera; José Gutiérrez; Abraham Zonana-Nacach; Ernesto A. Jiménez-Balderas

    2008-01-01

    Objetivo. Investigar la asociación del antecedente de parasitosis intestinal extrema (PIE) durante la infancia enpacientes ahora adultos con enfermedades reumáticas/ autoinmunes (ERAI). Métodos. Se estudiaron 54 pacientes consecutivos con ERAI, (27 con espondilitis anquilosante 9 con artritis reumatoide, 19 con lupus eritematoso sistémico) y 54 controles sanos; 27 hombres y 27 mujeres. Todos los sujetos tenían padres que informaran de eventos PIE durante la infancia, previos al inicio de la E...

  2. The epidemiology of extra-articular manifestations in ankylosing spondylitis

    DEFF Research Database (Denmark)

    Stolwijk, Carmen; Essers, Ivette; van Tubergen, Astrid;

    2015-01-01

    OBJECTIVE: To assess the incidence and risks of common extra-articular manifestations (EAMs), that is, acute anterior uveitis (AAU), psoriasis and inflammatory bowel disease (IBD), in patients with ankylosing spondylitis (AS) compared with population-based controls. METHODS: All incident patients...

  3. Computational aspects in mechanical modeling of the articular cartilage tissue.

    Science.gov (United States)

    Mohammadi, Hadi; Mequanint, Kibret; Herzog, Walter

    2013-04-01

    This review focuses on the modeling of articular cartilage (at the tissue level), chondrocyte mechanobiology (at the cell level) and a combination of both in a multiscale computation scheme. The primary objective is to evaluate the advantages and disadvantages of conventional models implemented to study the mechanics of the articular cartilage tissue and chondrocytes. From monophasic material models as the simplest form to more complicated multiscale theories, these approaches have been frequently used to model articular cartilage and have contributed significantly to modeling joint mechanics, addressing and resolving numerous issues regarding cartilage mechanics and function. It should be noted that attentiveness is important when using different modeling approaches, as the choice of the model limits the applications available. In this review, we discuss the conventional models applicable to some of the mechanical aspects of articular cartilage such as lubrication, swelling pressure and chondrocyte mechanics and address some of the issues associated with the current modeling approaches. We then suggest future pathways for a more realistic modeling strategy as applied for the simulation of the mechanics of the cartilage tissue using multiscale and parallelized finite element method.

  4. Early micromovement of the Articular Surface Replacement (ASR) femoral component

    DEFF Research Database (Denmark)

    Penny, J O; Ding, M; Varmarken, Jens-Erik;

    2012-01-01

    Radiostereometric analysis (RSA) can detect early micromovement in unstable implant designs which are likely subsequently to have a high failure rate. In 2010, the Articular Surface Replacement (ASR) was withdrawn because of a high failure rate. In 19 ASR femoral components, the mean micromovement...

  5. Evidence for a negative Pasteur effect in articular cartilage.

    Science.gov (United States)

    Lee, R B; Urban, J P

    1997-01-01

    Uptake of external glucose and production of lactate were measured in freshly-excised bovine articular cartilage under O2 concentrations ranging from 21% (air) to zero (N2-bubbled). Anoxia (O2 concentration Pasteur effect in bovine articular cartilage. Anoxia also suppressed glycolysis in articular cartilage from horse, pig and sheep. Inhibitors acting on the glycolytic pathway (2-deoxy-D-glucose, iodoacetamide or fluoride) strongly decreased aerobic lactate production and ATP concentration, consistent with the belief that articular cartilage obtains its principal supply of ATP from substrate-level phosphorylation in glycolysis. Azide or cyanide lowered the ATP concentration in aerobic cartilage to approximately the same extent as did anoxia but, because glycolysis (lactate production) was also inhibited by these treatments, the importance of any mitochondrial ATP production could not be assessed. A negative Pasteur effect would make chondrocytes particularly liable to suffer a shortage of energy under anoxic conditions. Incorporation of [35S]sulphate into proteoglycan was severely curtailed by treatments, such as anoxia, which decreased the intracellular concentration of ATP.

  6. Surgery for extra-articular trismus : a systematic review

    NARCIS (Netherlands)

    Bouman, M A; Dijkstra, P U; Reintsema, H; Roodenburg, J L N; Werker, P M N

    2015-01-01

    The aim of this systematic review was to identify operations that are used to improve mouth opening in patients with extra-articular trismus (caused by cancer and its treatment, oral submucous fibrosis, or noma) and to find out if they work. We searched the electronic databases PubMed, Embase, Cinah

  7. Surgery for extra-articular trismus : a systematic review

    NARCIS (Netherlands)

    Bouman, M. A.; Dijkstra, P. U.; Reintsema, H.; Roodenburg, J. L. N.; Werkera, P. M. N.

    2016-01-01

    The aim of this systematic review was to identify operations that are used to improve mouth opening in patients with extra-articular trismus (caused by cancer and its treatment, oral submucous fibrosis, or noma) and to find out if they work. We searched the electronic databases PubMed, Embase, Cinah

  8. FACTOR NUCLEAR κB (NF-κB): SIGNALOSOMA Y SU IMPORTANCIA EN ENFERMEDADES INFLAMATORIAS Y CÁNCER

    OpenAIRE

    Nancy P Echeverri R; Ismena Mockus S

    2008-01-01

    El factor nuclear κB (NF-κB) es un dímero constituido por proteínas de la familia Rel. El NF-κB se encuentra en el citoplasma unido a proteínas inhibidoras (IkB). Las IkB son fosforiladas por diferentes cinasas que hacen parte del signalosoma como las cinasas de IKKα e IKKP y el modulador esencial de NF-κB (NEMO), la proteína cinasa activadora de mitosis (MAPK o p38) y la cinasa inductora de NF-κB (NIK). Estas cinasas al ser activadas por señales dependientes de citocinas y luz ultravioleta, ...

  9. Asociación HLA y artritis reumatoidea juvenil en busca de las bases moleculares dependiente del MHC /

    OpenAIRE

    Garavito de Egea, Gloria

    2004-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada Artritis reumatoidea Juvenil (ARJ) es una enfermedad inflamatoria crónica, autoinmune que afecta a mas de una articulación en lugar y numero. Es una de las enfermedades más comunes en la consulta pediátrica reumatológica y una de las menos estudiadas desde el punto de vista inmunogenetico. En la literatura se ha reportado varias asociaciones de (HLA) Antigenos de Leucocitos Humanos y ARJ con diferentes grupos étnicos, so...

  10. The Effectiveness of Ultrasound-Guided Steroid Injection for Femoroacetabular Impingement: A Comparison between the Extra-Articular and Intra-Articular Approaches

    Energy Technology Data Exchange (ETDEWEB)

    Jeon, Chang Ho; Lee, Joon Woo [Dept. of Radiology, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of)

    2013-03-15

    To assess the effectiveness of pain control using ultrasound-guided steroid injection by the extra-articular and intra-articular approaches to femoroacetabular impingement patients. From September through December 2010, 18 patients with clinical suspicion of femoroacetabular impingement syndrome underwent ultrasound-guided steroid and local anesthetics injection: a total of 20 cases, including 16 cases of ipsilateral and 2 cases of bilateral injection. Extra-articular or intra-articular approach was selected for each patient, randomly and alternately. Nine cases were performed by extra-articular approach and 11 cases were performed by intra-articular approach. Every patient was observed in the outpatient clinic and visual analogue scale (VAS) was taken and compared before and after the procedure in all patients. Pre-injection average VAS value of extra-articular approached cases was 5.22 {+-} 1.99 and post-injection average VAS value was 4.11 {+-} 1.96, which is statistically insignificant (p < 0.156). The average VAS value of intra-articular approached cases was decreased from 5.72 {+-} 2.15 to 2.91 {+-} 2.30 after injection, which is statistically significant (p < 0.006). Ultrasound guided intra-articular approached steroid and local anesthetics injection could be effective in controlling pain for patients with femoroacetabular impingement syndrome.

  11. Influencia del entrenamiento del equilibrio con plataforma dinamométrica en artroplastia total de rodilla

    OpenAIRE

    Roig-Casasús, Sergio

    2015-01-01

    Introducción: Debido al en envejecimiento de la población y al aumento en la esperanza de vida, en la actualidad la artroplastia de rodilla es una de las cirugías más frecuentes en el primer mundo. Las causas más frecuentes del reemplazo protésico son la artrosis y la artritis reumatoidea. Dependiendo del dolor, la limitación articular y la impotencia funcional, el cirujano determina dicha cirugía. Gran parte del éxito de esta cirugía tanto a nivel motor como funcional depende en gran medida ...

  12. One intra-articular injection of hyaluronan prevents cell death and improves cell metabolism in a model of injured articular cartilage in the rabbit

    NARCIS (Netherlands)

    Jansen, Edwin J. P.; Ernans, Pieter J.; Douw, Conny M.; Guidemond, Nick A.; Van Rhijn, Lodewijk W.; Bulstra, Sjoerd K.; Kuijer, Roell

    2008-01-01

    The purpose of this study was to determine the effect of one intra-articular injection of hyaluronan on chondrocyte death and metabolism in injured cartilage. Twenty-three 6-month-old rabbits received partial-thickness articular cartilage defects created on each medial femoral condyle. In order to e

  13. Reacción inflamatoria asociada a infiltración cosmética facial múltiple: A propósito de un caso Inflammatory reaction associated with multiple cosmetic facial infiltration: Report of a case

    OpenAIRE

    B Tejedor Gómez; J.C. Coca Meneses; J.C. Melendres Romero; A. Eguía del Valle; J. López Vicente; R. Martínez-Conde

    2010-01-01

    El uso de sustancias para el aumento de los tejidos blandos con fines cosméticos puede ocasionar la aparición de diferentes efectos adversos, entre los que se encuentran las reacciones inflamatorias tardías. Se presenta un caso clínico de una de estas reacciones en una paciente de 41 años de edad que había sido infiltrada 4 años antes en ambos surcos nasogenianos y glabelar con un gel de poliacrilamida y posteriormente con toxoide botulínico tipo A. Se discuten los principales aspectos clínic...

  14. Intra-articular Lidocaine Injection for Shoulder Reductions

    OpenAIRE

    Waterbrook, Anna L; Paul, Stephen

    2011-01-01

    Context: The shoulder is the most commonly dislocated joint, and shoulder dislocations are very common in sports. Many of these dislocations present to the office or training room for evaluation. Usual practice is an attempt at manual reduction without analgesia and then transfer to the emergency department if unsuccessful. The clinical efficacy of intra-articular lidocaine for reduction of anterior shoulder dislocations in the outpatient setting was examined. Evidence Acquisition: An OVID ME...

  15. Engineering articular cartilage using newly developed carrageenan basedhydrogels

    OpenAIRE

    Popa, Elena Geta

    2014-01-01

    Articular cartilage holds specific functionality in the human body creating smooth gliding areas and allowing the joints to move easily without pain. However, due to its avascular nature and to the low metabolic activity of the constituent cells-the chondrocytes, cartilage has a low regenerative potential. The current surgical options to treat damaged cartilage are not long lasting and involve frequent revisions. Tissue engineering may provide an alternative approach for cartilage...

  16. Advances in the Surgical Management of Articular Cartilage Defects

    OpenAIRE

    Stein, Spencer; Strauss, Eric; Bosco, Joseph

    2013-01-01

    Objective: The purpose of this review is to gain insight into the latest methods of articular cartilage implantation (ACI) and to detail where they are in the Food and Drug Administration approval and regulatory process. Design: A PubMed search was performed using the phrase “Autologous Chondrocyte Implantation” alone and with the words second generation and third generation. Additionally, clinicaltrials.gov was searched for the names of the seven specific procedures and the parent company we...

  17. Familial osteochondritis dissecans a dysplasia of articular cartilage

    Energy Technology Data Exchange (ETDEWEB)

    Kozlowski, K.; Middleton, R.

    1985-03-01

    Two cases of osteochondritis dissecans in a boy and his mother are described. In the son only the knee and elbow joints and the epiphysis of the right second metacarpal were involved, whereas the mother showed early generalised degenerative joint disease associated with many large loose bodies in one knee. The authors speculate that familial osteochondritis dissecans is a dysplasia of articular cartilage caused by abnormal chondrocyte metabolism.

  18. Depth Dependence of Shear Properties in Articular Cartilage

    Science.gov (United States)

    Buckley, Mark; Gleghorn, Jason; Bonassar, Lawrence; Cohen, Itai

    2007-03-01

    Articular cartilage is a highly complex and heterogeneous material in its structure, composition and mechanical behavior. Understanding these spatial variations is a critical step in designing replacement tissue and developing methods to diagnose and treat tissue affected by damage or disease. Existing techniques in particle image velocimetry (PIV) have been used to map the shear properties of complex materials; however, these methods have yet to be applied to understanding shear behavior in cartilage. In this talk, we will show that confocal microscopy in conjunction with PIV techniques can be used to determine the depth dependence of the shear properties of articular cartilage. We will show that the shear modulus of this tissue varies by over an order of magnitude over its depth, with the least stiff region located about 200 microns from the surface. Furthermore, our data indicate that the shear strain profile of articular cartilage is sensitive to both the degree of compression and the total applied shear strain. In particular, we find that cartilage strain stiffens most dramatically in a region 200-500 microns below the surface. Finally, we will describe a physical model that accounts for this behavior by taking into account the local buckling of collagen fibers just below the cartilage surface and present second harmonic generation (SHG) imaging data addressing the collagen orientation before and after shear.

  19. Effects of vimentin disruption on the mechanoresponses of articular chondrocyte.

    Science.gov (United States)

    Chen, Cheng; Yin, Li; Song, Xiongbo; Yang, Hao; Ren, Xiang; Gong, Xiaoyuan; Wang, Fuyou; Yang, Liu

    2016-01-01

    Human articular cartilage is subjected to repetitive mechanical loading during life time. As the only cellular component of articular cartilage, chondrocytes play a key role in the mechanotransduction within this tissue. The mechanoresponses of chondrocytes are largely determined by the cytoskeleton. Vimentin intermediate filaments, one of the major cytoskeletal components, have been shown to regulate chondrocyte phenotype. However, the contribution of vimentin in chondrocyte mechanoresponses remains less studied. In this study, we seeded goat articular chondrocytes on a soft polyacrylamide gel, and disrupted the vimentin cytoskeleton using acrylamide. Then we applied a transient stretch or compression to the cells, and measured the changes of cellular stiffness and traction forces using Optical Magnetic Twisting Cytometry and Traction Force Microscopy, respectively. In addition, to study the effects of vimentin disruption on the intracellular force generation, we treated the cells with a variety of reagents that are known to increase or decrease cytoskeletal tension. We found that, after a compression, the contractile moment and cellular stiffness were not affected in untreated chondrocytes, but were decreased in vimentin-disrupted chondrocytes; after a stretch, vimentin-disrupted chondrocytes showed a lower level of fluidization-resolidification response compared to untreated cells. Moreover, vimentin-disrupted chondrocytes didn't show much difference to control cells in responding to reagents that target actin and ROCK pathway, but showed a weaker response to histamine and isoproterenol. These findings confirmed chondrocyte vimentin as a major contributor in withstanding compressive loading, and its minor role in regulating cytoskeletal tension. PMID:26616052

  20. Efectividad y seguridad de la viscosuplementación con dosis única de ácido hialurónico estabilizado de origen no animal (NASHA) para el tratamiento del dolor secundario a gonartrosis Efficacy and safety of single intra-articular injection of non-animal stabilized hyaluronic acid (NASHA) for gonarthrosis pain treatment

    OpenAIRE

    M.M. Monerris Tabasco; I. Martí Acebedo; G. Roca Amatria; R. M. Rincón Párraga; M. Hinojosa Zaguirre; Y. Jiménez Capel; D. Samper Bernal

    2012-01-01

    Objetivos: valorar la efectividad y seguridad de la inyección de dosis única de ácido hialurónico estabilizado de origen no animal (NASHA) para el tratamiento del dolor secundario a gonartrosis. Material y métodos: se han incluido un total de 37 pacientes afectos de gonartrosis diagnosticada previamente por el Traumatólogo/Reumatólogo en los que ha fracasado el tratamiento conservador y/o que no son tributarios de tratamiento quirúrgico. Se les ha efectuado una infiltración en dosis única de ...

  1. Efecto del tratamiento con atorvastatina sobre los marcadores de inflamación en la cirugía de revascularización coronaria con arteria mamaria

    OpenAIRE

    Parraza Díez, Naiara

    2012-01-01

    INTRODUCCIÓN Recientes estudios muestran que la respuesta inflamatoria que tiene lugar durante la cirugía vascular está lejos de ser duradera, encontrando un aumento de hasta 20veces la concentración de los marcadores de inflamación y siendo detectable este aumento en algunos de ellos hasta 24h después de la cirugía. Mediciones seriadas de diferentes marcadores de inflamación parecen ser útiles para cuantificar el grado de daño tisular, la invasividad del procedimiento y la detección temprana...

  2. Estudio del estado de salud oral y salival en relación con las complicaciones de diabéticos tipo 1

    OpenAIRE

    Hontanilla Estruch, Estela

    2014-01-01

    En la actualidad está reconocido que la patogénesis de la diabetes está muy influenciada por una activación del sistema inmune y por un bajo grado de inflamación. Las principales citoquinas que participan en la patogénesis de la diabetes son la IL-1, el TNF- , y la IL-6. Además, algunos estudios recientes han demostrado que la inflamación, y más concretamente las citoquinas inflamatorias, son determinantes en el desarrollo de las complicaciones microvasculares de la diabetes: neuropatía, reti...

  3. Esclerosis múltiple en una mujer fallecida por quemaduras en el incendio del domicilio Multiple sclerosis in a woman died by burns in a house fire

    OpenAIRE

    J. Lucena; M. Salguero; A. Rico; Blanco, M.; Marín, R; E. Barrero; F. Heredia

    2004-01-01

    La esclerosis múltiple (EM) o esclerosis en placas es la más común de las enfermedades inflamatorias desmielinizantes del SNC y el trastorno neurológico más incapacitante en los adultos jóvenes. Se caracteriza por la aparición de lesiones focales diseminadas en la sustancia blanca denominadas placas en las que lo más llamativo es la pérdida de la mielina (desmielinización) con preservación relativa de los axones. Es infrecuente que esta enfermedad entre en el campo de investigación médico-for...

  4. INTERNAL FIXATION OF INTRA ARTICULAR CALCANEUM FRACTURES USING LOCKING COMPRESSION PLATE

    OpenAIRE

    Neelakrishnan; Balamurugavel; Barathiselvan; Viswanathan

    2014-01-01

    INTRODUCTION: Calcaneum is the most frequently fractured tarsal bone. Historically intra articular calcaneum fractures treated non-operatively which led to increased morbidity due to in congruency of articular surface resulting in subtalar arthritis. With advent of CT scan, better implants and improved methods of fixation. Operative treatment has now become a standard method. AIM OF THE STUDY: To study the functional and radiological outcome of intra articular calcaneum fr...

  5. Intra-articular osteoid osteoma at the femoral trochlea treated with osteochondral autograft transplantation

    Science.gov (United States)

    Leeman, Joshua J; Motamedi, Daria; Wildman-Tobriner, Ben; O’Donnell, Richard J; Link, Thomas M

    2016-01-01

    We present the case of an intra-articular osteoid osteoma at the femoral trochlea. Intra-articular osteoid osteoma can present a diagnostic challenge both clinically and with imaging because it presents differently from the classic cortical osteoid osteoma. Given the lesion’s proximity to overlying cartilage, the patient underwent resection of the lesion with osteochondral autograft transplantation at the surgical defect. A comprehensive literature review and discussion of intra-articular osteoma will be provided. PMID:27761182

  6. Biochemical composition of the superficial layer of articular cartilage.

    Science.gov (United States)

    Crockett, R; Grubelnik, A; Roos, S; Dora, C; Born, W; Troxler, H

    2007-09-15

    To gain more information on the mechanism of lubrication in articular joints, the superficial layer of bovine articular cartilage was mechanically removed in a sheet of ice that formed on freezing the cartilage. Freeze-dried samples contained low concentrations of chondroitin sulphate and protein. Analysis of the protein by SDS PAGE showed that the composition of the sample was comparable to that of synovial fluid (SF). Attenuated total reflection infrared (ATR-IR) spectroscopy of the dried residue indicated that the sample contained mostly hyaluronan. Moreover, ATR-IR spectroscopy of the upper layer of the superficial layer, adsorbed onto silicon, showed the presence of phospholipids. A gel could be formed by mixing hyaluronan and phosphatidylcholine in water with mechanical properties similar to those of the superficial layer on cartilage. Much like the superficial layer of natural cartilage, the surface of this gel became hydrophobic on drying out. Thus, it is proposed that the superficial layer forms from hyaluronan and phospholipids, which associate by hydrophobic interactions between the alkyl chains of the phospholipids and the hydrophobic faces of the disaccharide units in hyaluronan. This layer is permeable to material from the SF and the cartilage, as shown by the presence of SF proteins and chondroitin sulphate. As the cartilage dries out after removal from the joint, the phospholipids migrate towards the surface of the superficial layer to reduce the surface tension. It is also proposed that the highly efficient lubrication in articular joints can, at least in part, be attributed to the ability of the superficial layer to adsorb and hold water on the cartilage surface, thus creating a highly viscous boundary protection.

  7. Metabolic Effects of Avocado/Soy Unsaponifiables on Articular Chondrocytes

    Directory of Open Access Journals (Sweden)

    Louis Lippiello

    2008-01-01

    Full Text Available Avocado/soy unsaponifiable (ASU components are reported to have a chondroprotective effect by virtue of anti-inflammatory and proanabolic effects on articular chondrocytes. The identity of the active component(s remains unknown. In general, sterols, the major component of unsaponifiable plant material have been demonstrated to be anti-inflammatory in vitro and in animal models. These studies were designed to clarify whether the sterol content of ASU preparations were the primary contributors to biological activity in articular chondrocytes. ASU samples were analyzed by high pressure liquid chromatography (HPLC and GC mass spectrometry. The sterol content was normalized between diverse samples prior to in vitro testing on bovine chondrocytes. Anabolic activity was monitored by uptake of 35-sulfate into proteoglycans and quantitation of labeled hydroxyproline and proline content after incubation with labeled proline. Anti-inflammatory activity was assayed by measuring reduction of interleukin-1 (IL-1-induced synthesis of PGE2 and metalloproteases and release of label from tissue prelabeled with S-35.All ASU samples exerted a similar time-dependent up-regulation of 35-sulfate uptake in bovine cells reaching a maximum of greater than 100% after 72 h at sterol doses of 1–10 μg/ml. Non-collagenous protein (NCP and collagen synthesis were similarly up-regulated. All ASU were equally effective in dose dependently inhibiting IL-1-induced MMP-3 activity (23–37%, labeled sulfate release (15–23% and PGE2 synthesis (45–58%. Up-regulation of glycosaminoglycan and collagen synthesis and reduction of IL-1 effects in cartilage are consistent with chondroprotective activity. The similarity of activity of ASU from diverse sources when tested at equal sterol levels suggests sterols are important for biologic effects in articular chondrocytes.

  8. Membrane channel gene expression in human costal and articular chondrocytes.

    Science.gov (United States)

    Asmar, A; Barrett-Jolley, R; Werner, A; Kelly, R; Stacey, M

    2016-04-01

    Chondrocytes are the uniquely resident cells found in all types of cartilage and key to their function is the ability to respond to mechanical loads with changes of metabolic activity. This mechanotransduction property is, in part, mediated through the activity of a range of expressed transmembrane channels; ion channels, gap junction proteins, and porins. Appropriate expression of ion channels has been shown essential for production of extracellular matrix and differential expression of transmembrane channels is correlated to musculoskeletal diseases such as osteoarthritis and Albers-Schönberg. In this study we analyzed the consistency of gene expression between channelomes of chondrocytes from human articular and costal (teenage and fetal origin) cartilages. Notably, we found 14 ion channel genes commonly expressed between articular and both types of costal cartilage chondrocytes. There were several other ion channel genes expressed only in articular (6 genes) or costal chondrocytes (5 genes). Significant differences in expression of BEST1 and KCNJ2 (Kir2.1) were observed between fetal and teenage costal cartilage. Interestingly, the large Ca(2+) activated potassium channel (BKα, or KCNMA1) was very highly expressed in all chondrocytes examined. Expression of the gap junction genes for Panx1, GJA1 (Cx43) and GJC1 (Cx45) was also observed in chondrocytes from all cartilage samples. Together, this data highlights similarities between chondrocyte membrane channel gene expressions in cells derived from different anatomical sites, and may imply that common electrophysiological signaling pathways underlie cellular control. The high expression of a range of mechanically and metabolically sensitive membrane channels suggest that chondrocyte mechanotransduction may be more complex than previously thought. PMID:27116676

  9. Osteochondritis dissecans of the subtalar articular facet: an unusual diagnosis

    Energy Technology Data Exchange (ETDEWEB)

    Madi, Faouzi; Vialle, Raphael; Mary, Pierre; Damsin, Jean-Paul; Filipe, Georges [Armand Trousseau Hospital, Department of Paediatric Orthopaedics, Paris Cedex 12 (France); Ducou le Pointe, Hubert [Armand Trousseau Hospital, Department of Radiology, Paris (France)

    2005-08-01

    Osteochondritis dissecans of the talus is classically characterized by an abnormality of the articular cartilage of the talar dome. We report a rare case of a lesion of the subtalar facet in a 10-year-old boy. The lesion was responsible for a painful ankle with secondary instability. The diagnosis was made using plain radiographs and CT, which showed a well-circumscribed osteochondral fragment demarcated from the adjacent bone by a radiolucent line. The clinical outcome was favourable after a 6-week period with a non-weight-bearing short-leg cast. (orig.)

  10. Granulocitoaféresis en la enfermedad inflamatoria intestinal: Eficacia a 32 semanas con protocolo de inducción y sesiones de mantenimiento Granulocytapheresis in inflamatory bowel disease: Efficacy of an induction plus maintenance sessions protocol at 32 weeks

    Directory of Open Access Journals (Sweden)

    F. J. Fernández Pérez

    2007-11-01

    Full Text Available Introducción: la granulocitoaféresis (GCAF retira de la circulación granulocitos-monocitos activados, modificando el pool circulante e induciendo una reducción en la inflamación intestinal. Objetivo: valorar la eficacia de la GCAF en la enfermedad inflamatoria intestinal (EII mediante sesiones de inducción y mantenimiento. Material y método: análisis retrospectivo en pacientes con EII activa corticodependiente y corticorrefractaria. Aplicamos 5 sesiones de inducción en colitis ulcerosa (CU y 7 en enfermedad de Crohn (EC y 1 sesión mensual hasta semana 32. Evaluamos indices clínicos de actividad y empleo de corticoides. Resultados: incluimos a 18 pacientes (10 CU, 8 EC, 10 de ellos corticodependientes y 8 corticorrefractarios. Catorce pacientes eran refractarios y 4 intolerantes a inmunosupresores (IS. No completaron la inducción 2-CU (brotes graves y 1-EC (secundarismos. Abandonaron el mantenimiento 1-CU y 3-EC. De aquellos que completaron inducción alcanzaron respuesta o remisión el 87,5% de las CU (2 y 5 pacientes, y el 71,4% de EC (1 y 4 pacientes respectivamente. Estos resultados a 32 semanas eran del 75% en CU (3 y 3 pacientes y del 42,8% en EC (1 y 2 pacientes respectivamente. De los que completaron la inducción, suprimieron corticoides el 14,2% de EC por el 62,5% de CU (25% de estas en remisión y 37,5% en respuesta. Registramos 2 secundarismos graves (tromboflebitis y síncope. Ninguna CU que completó inducción sufrió colectomía tras 97,6 (72-128 semanas de seguimiento. Conclusiones: tanto CU como EC responden bien a inducción con GCAF pero mientras la CU mantiene tras 32 semanas tasas similares de respuesta-remisión (87,5 vs. 75% casi 1/3 de los pacientes con EC recaen tras alcanzar respuesta. La granulocitoaféresis es una alternativa de tratamiento para inducir y mantener remision en CU, ahorrando corticoides, pero en la EC se precisa una adecuada selección de pacientes y un esquema de mantenimiento aún por

  11. Initial approach of patients admitted to third level hospitals with systemic inflamatory response syndrome Enfoque inicial de los pacientes admitidos a hospitales de tercer nivel con síndrome de respuesta inflamatoria sistémica (SRIS

    Directory of Open Access Journals (Sweden)

    Fabián Alberto Jaimes Barragán

    2000-04-01

    . Laboratory requests and microbiological studies did not in every case correlate with the initial diagnosis and the empirical antibiotic therapy. Objetivo: conocer el enfoque inicial de los pacientes con diagnóstico de síndrome de respuesta inflamatoria sistémica de origen infeccioso (SRIS en unidades de urgencias. Diseño: estudio observacional analítico en una cohorte concurrente. Lugar de estudio: servicios de urgencias del Hospital Universitario San Vicente de Paúl (HUSVP y el Hospital General de Medellín Luz Castro de Gutiérrez (HGM. Pacientes: admitidos por urgencias con SRIS de etiología no traumática entre agosto de 1998 y marzo de 1999, de edad igual o superior a 14 años, y con sospecha de infección como uno de los principales diagnósticos de admisión. Mediciones: descripción de la frecuencia de enfermedades asociadas, factores de riesgo y exploración física básica, determinación y utilidad de ayudas diagnósticas más usadas; y asociación entre foco infeccioso, microbiología, uso previo de antibióticos y antibioterapia empírica inicial en la admisión. Se utilizaron Chi cuadrado o prueba exacta de Fisher para la comparación de proporciones. Resultados: fueron admitidos 502 pacientes. Los principales antecedentes fueron EPOC (21.5% y trauma o cirugía previa (18.7%. La toma de signos vitales se determinó de la siguiente forma: frecuencia cardíaca en 100%, frecuencia respiratoria en 94.8%, presión arterial en 99.2%, temperatura en 80.3% y escala de Glasgow en 75.7% de los pacientes. Las solicitudes de laboratorio fueron: recuento de leucocitos en 98.4%, radiografía de tórax en 71.1%, recuento de plaquetas en 94.4% y creatinina en 89% de pacientes. En 26.5% de los pacientes no se solicitó ningún tipo de cultivo; en los restantes los más solicitados fueron los hemocultivos en 48.8% del total de la cohorte, de los cuales hubo crecimiento en 19.2% de las muestras. En 22.3% de los pacientes había habido consumo previo de antibióticos y

  12. Listeria monocytogenes septic arthritis following intra-articular yttrium-90 therapy.

    OpenAIRE

    Wilson, A P; Prouse, P J; Gumpel, J. M.

    1984-01-01

    Listeria monocytogenes is a rare cause of septic arthritis, which usually occurs in a host compromised by systemic illness. Intra-articular irradiation with yttrium-90 is generally free of complication. We report a case of intra-articular sepsis of the knee joint by Listeria monocytogenes acquired under unusual circumstances.

  13. Pseudomonas aeruginosa septic arthritis of knee after intra-articular ozone injection.

    Science.gov (United States)

    Seyman, Derya; Ozen, Nevgun Sepin; Inan, Dilara; Ongut, Gozde; Ogunc, Dilara

    2012-07-01

    We describe a case of septic arthritis caused by Pseudomonas aeruginosa in an immunocompetent patient following intra-articular ozone injection into the knee. To the best of our knowledge, and after considering the current literature,we believe this case is unique as no other reports of septic arthritis caused by P. aeruginosa following intra-articular ozone injection has been made.

  14. [Systematization of the articular surfaces of the carpometacarpal joints (author's transl)].

    Science.gov (United States)

    el Bacha, A; Maillot, C

    1977-01-01

    A study, done on 100 hands, of the systematization of the articular surfaces of the carpometacarpal joints, clearly delineates the variability of circumference, dimesions, and relief of the articular facets. An attempt to draw general conclusions from this morphological study, in terms of arthrokinetics, leads to an understanding of the nature of the joints and the movements that are performed at this site.

  15. CLINICAL OUTCOME OF INTRA - ARTICULAR DISTAL RADIUS FRACTURES TREATED WITH PLATE AND SCREWS

    OpenAIRE

    Girish; Hosangadi; Suresh; Suryakanth

    2015-01-01

    BACKGROUND : Incidence of fractures of distal radius are increasing due to more geriatric population and road traffic accidents. The fundamental goal of distal radius fractures treatment is restoration of normal or near normal alignment and articular congruity. Restoration of the anatomy and articular surface may prevent the onset of arthritis ...

  16. Internal plate fixation versus plaster in displaced complete articular distal radius fractures, a randomised controlled trial

    NARCIS (Netherlands)

    M.A.M. Mulders (Marjolein A. M.); M.M.J. Walenkamp (Monique); J.C. Goslings (Carel); N.W.L. Schep (Niels)

    2016-01-01

    textabstractBackground: Of all distal radius fractures, 25 % are complete articular fractures (AO/OTA type C fractures). Two thirds of those fractures are displaced and require reduction. According to several International Guidelines, adequately reduced intra-articular distal radius fractures are be

  17. Influence of site and age on biochemical characteristics of the collagen network of equine articular cartilage

    NARCIS (Netherlands)

    Brama, P.A.J.; TeKoppele, J.M.; Bank, R.A.; Weeren, P.R. van; Barneveld, A.

    1999-01-01

    Objective - To determine variations in biochemical characteristics of equine articular cartilage in relation to age and the degree of predisposition for osteochondral disease at a specific site. Sample Population - Articular cartilage specimens from 53 horses 4 to 30 years old. Procedure - Healthy s

  18. A biphasic finite element study on the role of the articular cartilage superficial zone in confined compression

    OpenAIRE

    Guo, Hongqiang; Maher, Suzanne A; Torzilli, Peter A.

    2014-01-01

    The aim of this study was to investigate the role of the superficial zone on the mechanical behavior of articular cartilage. Confined compression of articular cartilage was modeled using a biphasic finite element analysis to calculate the one-dimensional deformation of the extracellular matrix (ECM) and movement of the interstitial fluid through the ECM and articular surface. The articular cartilage was modeled as an inhomogeneous, nonlinear hyperelastic biphasic material with depth and strai...

  19. Non-linear model for compression tests on articular cartilage.

    Science.gov (United States)

    Grillo, Alfio; Guaily, Amr; Giverso, Chiara; Federico, Salvatore

    2015-07-01

    Hydrated soft tissues, such as articular cartilage, are often modeled as biphasic systems with individually incompressible solid and fluid phases, and biphasic models are employed to fit experimental data in order to determine the mechanical and hydraulic properties of the tissues. Two of the most common experimental setups are confined and unconfined compression. Analytical solutions exist for the unconfined case with the linear, isotropic, homogeneous model of articular cartilage, and for the confined case with the non-linear, isotropic, homogeneous model. The aim of this contribution is to provide an easily implementable numerical tool to determine a solution to the governing differential equations of (homogeneous and isotropic) unconfined and (inhomogeneous and isotropic) confined compression under large deformations. The large-deformation governing equations are reduced to equivalent diffusive equations, which are then solved by means of finite difference (FD) methods. The solution strategy proposed here could be used to generate benchmark tests for validating complex user-defined material models within finite element (FE) implementations, and for determining the tissue's mechanical and hydraulic properties from experimental data.

  20. Delivering Agents Locally into Articular Cartilage by Intense MHz Ultrasound

    Science.gov (United States)

    Nieminen, Heikki J.; Ylitalo, Tuomo; Suuronen, Jussi-Petteri; Rahunen, Krista; Salmi, Ari; Saarakkala, Simo; Serimaa, Ritva; Hæggström, Edward

    2015-01-01

    There is no cure for osteoarthritis. Current drug delivery relies on systemic delivery or injections into the joint. Because articular cartilage (AC) degeneration can be local and drug exposure outside the lesion can cause adverse effects, localized drug delivery could permit new drug treatment strategies. We investigated whether intense megahertz ultrasound (frequency: 1.138 MHz, peak positive pressure: 2.7 MPa, Ispta: 5 W/cm2, beam width: 5.7 mm at −6 dB, duty cycle: 5%, pulse repetition frequency: 285 Hz, mechanical index: 1.1) can deliver agents into AC without damaging it. Using ultrasound, we delivered a drug surrogate down to a depth corresponding to 53% depth of the AC thickness without causing histologically detectable damage to the AC. This may be important because early osteoarthritis typically exhibits histopathologic changes in the superficial AC. In conclusion, we identify intense megahertz ultrasound as a technique that potentially enables localized non-destructive delivery of osteoarthritis drugs or drug carriers into articular cartilage. PMID:25922135

  1. Historias del arte y experiencias pedagógicas

    OpenAIRE

    Muñoz Cobeñas, Leticia Amelia

    2012-01-01

    El presente trabajo intenta articular la experiencia pedagógica con reflexiones en torno a la historia del arte, su renovación teórica y el acercamiento a diferentes mundos empíricos de las llamadas obras de arte. Desde la cátedra Epistemología de las ciencias sociales de la Facultad de Bellas Artes hemos reconocido los aportes interdisciplinares en la definición de una historia del arte que renueva e incorpora nuevas problemáticas y otros objetos de estudio surgidos al interior del propio...

  2. Tratamiento del dolor en la artritis reumatoide fundamentado en medicina basada en la evidencia Pain treatment in rheumatoid arthritis and evidence-based medicine

    Directory of Open Access Journals (Sweden)

    F. Neira

    2006-11-01

    , ciclosporina, sales de oro, azatioprina, infliximab, leflunomida y ciclofosfamida. La combinación de uno o más son eficaces y no más tóxicos que por separado. Los glucocorticoides a dosis bajas disminuyen la evolución del daño articular, no deben ser recomendados de rutina (R B. La infiltración articular con corticoides puede ser útil en el tratamiento de la AR. Se utiliza concomitantemente la administración de calcio, vitamina D y bifosfonatos (R B. El tratamiento quirúrgico está indicado cuando el dolor no mejora con medidas médicas y hay pérdida de función. Entre las complicaciones de la AR se encuentran: síndrome del túnel del carpo, vasculitis reumatoide, ataque de la región cervical y artritis séptica. No se deben olvidar las medidas nutricionales, terapia ocupacional (NE I, ejercicios, protección de las articulaciones y psicoterapia (NE I.The objective has been the elaboration of a practical document, based on the best available scientific evidence, that allows physicians to adopt adequately founded diagnostic techniques and effective treatment in Rheumatoid Arthritis (RA. A systematic search in Internet has been made, using the term "rheumatoid arthritis" and "artritis reumatoide", in different groups of elaboration and storage of clinical practice quides: National Guideline Clearinghouse HTTP://www.guideline.gov/compares/compares.aspx, CMA infobase, Primary Care Clinical Practice Guidelines and Fisterra - Directory of Clinical Guides in Spanish. Guides of clinical practice (GPC based in the evidence, meta-analysis and articles of greater relevance have been reviewed. RA is an inflammatory poliarthritis of undetermined aetiology, usually involving peripheral joints, with a symmetrical distribution. It affects functional and work capacity and increases mortality. The usual symptoms are arthralgia, morning stiffness, fatigue, weight loss and fever. It is considered that RA is present when 4 of the following 7 criteria are observed (American Rheumatism

  3. Efectos del tratamiento con anti-TNF-α, infliximab, sobre la resistencia insulínica, adipocinas (visfatina, leptina, adiponectina, resistina y apelina) y angiopoyetina-2 en pacientes con espondilitis anquilosante

    OpenAIRE

    Miranda Filloy, José Alberto

    2014-01-01

    RESUMEN: La Espondilitis Anquilosante (EA) es una enfermedad inflamatoria crónica que afecta fundamentalmente a la columna vertebral produciendo sacroileítis. Estos pacientes presentan una aterogénesis acelerada y resistencia insulínica, lo que favorece la disfunción endotelial e incrementa el riesgo cardiovascular. Hemos documentado que la infusión del anti-TNF-α infliximab en pacientes no diabéticos con EA mejora la sensibilidad insulínica y disminuye las concentraciones séricas de angio...

  4. Estudio del engrosamiento pleural residual en los derrames pleurales paraneumónicos y empiemas y su asociación con los marcadores de actividad neutrofílica

    OpenAIRE

    Soriano Sánchez, Teresa

    2009-01-01

    El derrame pleural paraneumónico es una causa frecuente de derrame pleural exudado. En la fisiopatología de los DP paraneumónicos se han implicado mecanismos inflamatorios y de alteración en el equilibrio de la fibrinolisis pleural. La respuesta inflamatoria pleural, iniciada por el mesotelio, provoca un aumento de la permeabilidad vascular y la liberación de mediadores inflamatorios, que favorecerá la formación de derrame pleural exudado y la invasión del espacio pleural por células inflamat...

  5. Enfermedades articulares y uveítis Articular diseases and uveitis

    Directory of Open Access Journals (Sweden)

    J.M. Benítez del Castillo

    2008-01-01

    Full Text Available La inflamación ocular es una manifestación clínica frecuente de múltiples enfermedades sistémicas autoinmunes, siendo de gran relevancia en las espondiloartropatías. Dentro del grupo de las espondiloartropatías existen diferentes entidades clínicas, asociándose a diferentes patrones de uveítis. Se han definido una serie de patrones discriminativos que relacionan formas concretas de uveítis con determinadas enfermedades sistémicas u oculares. La uveítis anterior aguda unilateral recidivante es la más frecuente en las espondiloartropatías, y puede ser la forma de inicio de una espondiloartropatía no diagnosticada previamente. La colaboración entre oftalmólogos y reumatólogos o internistas es fundamental para el correcto manejo y tratamiento de estos pacientes.Ocular inflammation is a common clinical manifestation related to several autoimmune systemic disorders, specially spondyloarthropaties. In this group, there are different clinical diseases that are related to special uveitic patterns. Several discriminative patterns have been defined that closely link uveitis with certain systemic or ophthalmic diseases. Unilateral recurrent anterior acute uveitis is the most frequent form of uveitis related to spondyloarthropaties, and is sometimes the initial manifestation of an undiagnosed spondyloarthropaty. The collaboration of ophthalmologists, rheumatologists and internal medicine specialists is very important for the correct management and treatment of these patients.

  6. Láser de baja potencia en el tratamiento de las calcificaciones de hombro Low power laser in the treatment of shoulder calcifications

    OpenAIRE

    A. Hernández Díaz; B. M. González Méndez; A. Orellana Molina; J.L. Martín Gil; J. Berty Tejeda

    2009-01-01

    Introducción: La artropatía o enfermedad por depósito de cristales es la entidad en la que el factor desencadenante de la respuesta inflamatoria es la presencia de microcristales en las estructuras articulares, pudiendo conducir con el tiempo a la lesión articular. No necesariamente se asocia a manifestaciones clínicas, y frecuentemente es un hallazgo radiológico casual. Objetivos: Se evaluó la eficacia del láser de baja potencia en pacientes con calcificaciones periarticulares de hombro con ...

  7. The opinion of patients with inflammatory bowel disease on healthcare received Opinión de los pacientes con enfermedad inflamatoria intestinal sobre la atención sanitaria recibida

    Directory of Open Access Journals (Sweden)

    F. Casellas

    2004-03-01

    information, care of family members, and healthcare resources accessibility/agility.Fundamento: un elemento a tener en cuenta al analizar un modelo de atención sanitaria dirigido a un grupo de población afecta de una enfermedad crónica es el de los recursos sanitarios necesarios, su utilización y la valoración que tienen los usuarios de ellos. En la enfermedad inflamatoria intestinal (EII los recursos sanitarios utilizados por los enfermos son numerosos y variados, pero no han sido evaluados. Diseño: se ha realizado una encuesta autorrellenable anónima de 24 preguntas básicas a cerca de datos generales de la enfermedad, quién les controla, cómo se programan las visitas, necesidad de atención urgente, cómo creen que se puede mejorar su control, etc. La encuesta se remitió al domicilio de 393 pacientes para su contestación y se les pidió que la devolvieran por correo. Resultados: doscientos treinta y siete pacientes devolvieron la encuesta cumplimentada. La mayoría de los pacientes eran controlados en un hospital, y sólo el 8,8% lo eran por el médico de cabecera o especialista de zona. El 92% de pacientes refieren que las visitas se programan de forma rutinaria, independientemente del estado clínico. El 79,6% de pacientes refiere haber acudido alguna vez a urgencias, siendo el motivo en el 25,2% de las ocasiones no saber qué hacer o no tener otro recurso disponible. Ello explica que el 38% de visitas al servicio de urgencias se podrían haber evitado con un mejor conocimiento de la enfermedad o una consulta telefónica. El 30% de pacientes refiere que la actual atención sanitaria es inadecuada, ya sea por su contenido, la forma o el tiempo de espera. El 97,8% de pacientes cree que la información y conocimiento de la enfermedad ayudarían a su control, de forma que el 69,6% considera que con la información adecuada podrían iniciar el tratamiento ya antes de la visita médica. Otro aspecto poco atendido, y que el 74,6% de pacientes opina que ayudaría al

  8. Estructuras articulares, funcionales y anatómicas en la técnica de rotura de poder en el Taekwon-do

    OpenAIRE

    Bulens, Marlene

    2013-01-01

    El objetivo de la siguiente ponencia, es examinar aspectos anatómicos-funcionales y biomecánicos necesarios para lograr un correcto gesto técnico de la muñeca y de la mano, durante el desarrollo de la técnica de rotura de poder con golpe de puño en el Taekwon-do que conlleva a una excesiva fuerza de compresión articular que resiste la muñeca y la mano además del constante ajuste de la técnica para reestablecer la estabilidad. Hay tensiones musculares producidas por los músculos del antebra...

  9. Contact mechanics of articular cartilage layers asymptotic models

    CERN Document Server

    Argatov, Ivan

    2015-01-01

    This book presents a comprehensive and unifying approach to articular contact mechanics with an emphasis on frictionless contact interaction of thin cartilage layers. The first part of the book (Chapters 1–4) reviews the results of asymptotic analysis of the deformational behavior of thin elastic and viscoelastic layers. A comprehensive review of the literature is combined with the authors’ original contributions. The compressible and incompressible cases are treated separately with a focus on exact solutions for asymptotic models of frictionless contact for thin transversely isotropic layers bonded to rigid substrates shaped like elliptic paraboloids. The second part (Chapters 5, 6, and 7) deals with the non-axisymmetric contact of thin transversely isotropic biphasic layers and presents the asymptotic modelling methodology for tibio-femoral contact. The third part of the book consists of Chapter 8, which covers contact problems for thin bonded inhomogeneous transversely isotropic elastic layers, and Cha...

  10. Intra-Articular Polyacrylamide Hydrogel Injections Are Not Innocent

    Directory of Open Access Journals (Sweden)

    Murat Tonbul

    2014-01-01

    Full Text Available Osteoarthritis is a chronic disorder characterized by joint cartilage degeneration with concomitant changes in the synovium and subchondral bone metabolism. Many conservative treatment modalities, one of which is intra-articular injections, have been described for the treatment of this disorder. Traditionally, hyaluranic acid and corticosteroids are the agents that have been used for this purpose. Recently, polyacrylamide hydrogels are being used widely. Biocompatibility, nonbioabsorbability, and anti-infectious effect obtained by silver addition made polyacrylamide hydrogels more popular. In this paper, we present a case and the method of our management, in whom host tissue reaction (foreign body granuloma, edema, inflammation, and redness induration has been observed, as the first and unique adverse effect reported in the literature.

  11. Effects of Cryoprotective Agents on the Bovine Articular Chondrocyte Viability

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Cryopreservation is the process of choice for long term preservation of cells and tissues. In this study, the effects of cryoprotective agents, dimethyl sulfoxide(DMSO), glycerol and 1,2-propanediol on the bovine articular chondrocyte viability were examined experimentally. The CPA was added at the concentrations of 0. 6. 0.9, 1.2 and 1.5 mol/I and at 4℃ and 37℃ and removed at 37℃ in one-step. CPA stepwise addition and removal at 0. 6 and 1. 2 mol/L and at 37℃ was also tested as an alternative protocol. Cell volume excursion during DMSO addition and removal was estimated and correlated well with cell survival rates. Solution makeup affects cell survival rate and a stepwise protocol can improve the cell survival rates significantly.

  12. Immortalization of human articular chondrocytes and induction of their phenotype

    Institute of Scientific and Technical Information of China (English)

    何清义; 李起鸿; 杨柳; 许建中

    2003-01-01

    Objective To immortalize human articular chondrocytes (HACs) using gene transfection and to maintain stable phenotype of transformed HACs after induction.Methods HACs were transfected with the retroviral vector pLXSN encoding human papillomavirus 16E7 (HPV16E7), and the transformed clones were sorted and proliferated. Karyotype analysis, clone forming tests and nude mice tumor forming tests were applied to check the characteristics of the transformation. Type Ⅱ collagen of transformed chondrocytes was inducted with free serum medium (FSM) supplemented with nutridoma-sp and ascorbate. Results Immortalized HACs were isolated with fifty passages achieved. The HPV16E7 transformed cells were confirmed to be benign. Induction of FSM with nutridoma-sp and ascorbate promoted type Ⅱ collagen of transformed chondrocytes to the high levels of normal chondrocytes. Conclusion HACs transformed with HPV16E7 survive for long periods in vitro, and type Ⅱ collagen can maintain stability after induction.

  13. [Gait analysis after intra-articular calcaneus fractures].

    Science.gov (United States)

    Siegmeth, A; Petje, G; Mittlmeier, T; Vécsei, V

    1996-01-01

    We retrospectively compared 20 patients with displaced intra-articular calcaneal fractures by clinical assessment and dynamic pedography. Eleven were treated operatively, 9 conservatively. The purpose was to identify differences in post-traumatic gait performance and to correlate the pedographic data to a clinical score to show its reliability. Twenty individuals without a history of foot injuries were used as a control group. Both groups had restricted motion in the subtalar joint, increased hindfoot and midfoot loading and decreased forefoot loading. Furthermore, they showed prolonged contact phases and an impaired ability to speed up gait during the toe-off phase. Load transfer from the hindfoot to the forefoot showed typical distribution patterns. The operatively treated group showed better functional results with fewer subjective complaints.

  14. Partial rotator cuff injury in athletes: bursal or articular?

    Directory of Open Access Journals (Sweden)

    Cassiano Diniz Carvalho

    2015-08-01

    Full Text Available ABSTRACTA painful shoulder is a very common complaint among athletes, especially in the case of those in sports involving throwing. Partial lesions of the rotator cuff may be very painful and cause significant functional limitation to athletes' sports practice. The incidence of partial lesions of the cuff is variable (13-37%. It is difficult to make the clinical and radiological diagnosis, and this condition should be borne in mind in the cases of all athletes who present symptoms of rotator cuff syndrome, including in patients who are diagnosed only with tendinopathy. OBJECTIVE: To evaluate the epidemiological behavior of partial lesions of the rotator cuff in both amateur and professional athletes in different types of sports. METHODS: We evaluated 720 medical files on athletes attended at the shoulder service of the Discipline of Sports Medicine at the Sports Traumatology Center, Federal University of São Paulo. The majority of them were men (65%. Among all the patients, 83 of them were diagnosed with partial lesions of the rotator cuff, by means of ultrasonography or magnetic resonance, or in some cases using both. We applied the binomial test to compare the proportions found. RESULT: It was observed that intra-articular lesions predominated (67.6% and that these occurred more frequently in athletes in sports involving throwing (66%. Bursal lesions occurred in 32.4% of the athletes, predominantly in those who did muscle building (75%. CONCLUSION: Intra-articular lesions are more frequent than bursal lesions and they occur predominantly in athletes in sports involving throwing, while bursal lesions were more prevalent in athletes who did muscle building.

  15. Multiphysical modelling of fluid transport through osteo-articular media

    Directory of Open Access Journals (Sweden)

    Thibault Lemaire

    2010-03-01

    Full Text Available In this study, a multiphysical description of fluid transport through osteo-articular porous media is presented. Adapted from the model of Moyne and Murad, which is intended to describe clayey materials behaviour, this multiscale modelling allows for the derivation of the macroscopic response of the tissue from microscopical information. First the model is described. At the pore scale, electrohydrodynamics equations governing the electrolyte movement are coupled with local electrostatics (Gauss-Poisson equation, and ionic transport equations. Using a change of variables and an asymptotic expansion method, the macroscopic description is carried out. Results of this model are used to show the importance of couplings effects on the mechanotransduction of compact bone remodelling.Neste estudo uma descrição multifísica do transporte de fluidos em meios porosos osteo articulares é apresentada. Adaptado a partir do modelo de Moyne e Murad proposto para descrever o comportamento de materiais argilosos a modelagem multiescala permite a derivação da resposta macroscópica do tecido a partir da informação microscópica. Na primeira parte o modelo é apresentado. Na escala do poro as equações da eletro-hidrodinâmica governantes do movimento dos eletrolitos são acopladas com a eletrostática local (equação de Gauss-Poisson e as equações de transporte iônico. Usando uma mudança de variáveis e o método de expansão assintótica a derivação macroscópica é conduzida. Resultados do modelo proposto são usados para salientar a importância dos efeitos de acoplamento sobre a transdução mecânica da remodelagem de ossos compactados.

  16. Extra-Articular Manifestations of Rheumatoid Arthritis, Now

    Directory of Open Access Journals (Sweden)

    Paloma Vela

    2014-06-01

    Full Text Available Rheumatoid arthritis (RA is a chronic systemic inflammatory disease, characterised by polyarthritis and extra-articular organ disease, including rheumatoid nodules, ophthalmologic manifestations, cardiopulmonary disease, vasculitis, neuropathy, glomerulonephritis, Felty’s syndrome, and amyloidosis. Extra-articular manifestations of RA (ExRA occur in 17.8–40.9% of RA patients, 1.5–21.5% of them presenting as severe forms and usually associated with increased morbidity and mortality. They can develop at any time during the course of the disease, even in the early stages, and are associated with certain predisposing factors, such as the presence of rheumatoid factor, smoking, and long-standing severe disease. Rheumatoid nodules, the most common ExRA, have been found to be associated with the development of severe features, such as vasculitis, rheumatoid lung disease, pericarditis, and pleuritis, especially in those patients who develop them within 2 years from RA diagnosis. There is no uniformity in the definition of the term ExRA, which limits comparability between different studies. Several recent surveys suggest a lower frequency, probably due to a better control of disease activity. Diagnosis of ExRA is a challenge for clinicians, given its variable and complex presentation, and the lack of specific diagnostic tests; it must be based on clinical recognition and exclusion of other causes of the signs and symptoms. Furthermore, management continues to be difficult with a bad prognosis in many conditions. This article reviews the clinical aspects of major ExRA, focusing on incidence, clinical features, and therapeutic approaches, and how modern immunosuppressive therapy can change the outcome.

  17. Effects of immobilization on thickness of superficial zone of articular cartilage of patella in rats

    Directory of Open Access Journals (Sweden)

    Khadija Iqbal

    2012-01-01

    Conclusion: Each segment of superficial zone behaves differentially on immobilization and remobilization. Perhaps a much longer duration of remobilization is required to reverse changes of immobilization in articular cartilage and plays a significant role in knee joint movements.

  18. Estimation of inflammation by Doppler ultrasound: quantitative changes after intra-articular treatment in rheumatoid arthritis

    OpenAIRE

    Terslev, L; Torp-Pedersen, S; Qvistgaard, E; Danneskiold-Samso..., B; BLIDDAL, H

    2003-01-01

    Objective: To evaluate the use of ultrasound, including quantitative Doppler analysis of synovial vascularisation, before and after intra-articular treatment with glucocorticosteroids in patients with rheumatoid arthritis (RA).

  19. Tratamiento con electrocauterización de las lesiones premalignas del cérvix

    Directory of Open Access Journals (Sweden)

    Israel Meza G

    2014-11-01

    Full Text Available Se presentaron los resultados del tratamiento con electrocauterización de las neoplasias intraepiteliales del cérvix en 430 pacientes atendidas en el Hospital Universitario del Valle, en el Hospital San Juan de Dios y en el Centro Hospital Cañaveralejo en Cali, Colombia, entre 1982 y 1992. Inicialmente se seleccionaron 455 mujeres que consultaron con citologías anormales (inclusive Papanicolau II con cambios coilocíticos y atipias inflamatorias. Para el análisis final sólo se incluyeron 430 pacientes. A todas se les practicó colposcopia y biopsia dirigida y se confirmaba el diagnóstico de neoplasia intraepitelial del cérvix. Se hizo seguimiento con citología y colposcopia. Se obtuvo una respuesta global de 90.1% al primer tratamiento, respuesta que aumenta a 96.8% cuando se incluyeron las pacientes que respondieron a un segundo tratamiento. Se discuten las ventajas y desventajas del método con respeto a otros métodos existentes.

  20. The Variations in Calcaneal Articular Facets In North Indian Population and its Clinical Implication

    OpenAIRE

    Deepinder Kaur Gandhi; Maninder Singh; Anupama Mahajan; Seema

    2012-01-01

    Aims and Objectives- To know the most common type of calcanei in North Indian population and itsclinical importance. There are three articular facets on superior surface of calcaneus- anterior, middle andposterior. Three types of calcanei are noted according to number and arrangement of the articular facets-type A, B and C. Methodology - The present studywas done on 300 dry adult human calcanei of unknownsex taken from Department of Anatomy Sri Guru Ram Das Institute of Medical Sciences and R...

  1. Modulation of Apoptosis and Differentiation by the Treatment of Sulfasalazine in Rabbit Articular Chondrocytes

    OpenAIRE

    Lee, Won Kil; Kang, Jin Seok

    2016-01-01

    This study was conducted to examine the cellular regulatory mechanisms of sulfasalazine (SSZ) in rabbit articular chondrocytes treated with sodium nitroprusside (SNP). Cell phenotype was determined, and the MTT assay, Western blot analysis and immunofluorescence staining of type II collagen was performed in control, SNP-treated and SNP plus SSZ (50~200 μg/mL) rabbit articular chondrocytes. Cellular proliferation was decreased significantly in the SNP-treated group compared with that in the co...

  2. Diagnosis and management of an intra-articular foreign body in the foot.

    LENUS (Irish Health Repository)

    Mulhall, K J

    2002-10-01

    We describe a case of a small intra-articular foreign body in the foot presenting 48 hours following injury, which at operation showed early evidence of septic arthritis. It is essential to accurately localise periarticular foreign bodies in the foot and proceed to arthrotomy and debridement in all cases where there is radiological or clinical evidence to suggest intra-articular retention of a foreign body.

  3. Protein-based injectable hydrogels towards the regeneration of articular cartilage

    OpenAIRE

    Poveda Reyes, Sara

    2016-01-01

    [EN] Articular cartilage is a tissue with low capacity for self-restoration due to its avascularity and low cell population. It is located on the surface of the subchondral bone covering the diarthrodial joints. Degeneration of articular cartilage can appear in athletes, in people with genetic degenerative processes (osteoarthritis or rheumatoid arthritis) or due to a trauma; what produces pain, difficulties in mobility and progressive degeneration that finally leads to joint failure. Self-re...

  4. The use of dynamic culture devices in articular cartilage tissue engineering.

    OpenAIRE

    Akmal, M.

    2006-01-01

    Tissue engineered repair of articular cartilage has now become a clinical reality with techniques for cell culture having advanced from laboratory experimentation to clinical application. Despite the advances in the use of this technology in clinical applications, the basic cell culture techniques for autologous chondrocytes are still based on primitive in-vitro monolayer culture methods. Articular chondrocytes are known to undergo fibroblastic change in monolayer culture as this is not their...

  5. Role of computerised tomography in management of intra-articular fractures of the os calcis

    OpenAIRE

    Kumar, V.; Hameed, A.; Bhattacharya, R.; McMurtry, I.

    2006-01-01

    This study aimed to look at the role played by the CT scan in decision making in the management of intra-articular fractures of the calcaneum. Twenty-four patients with intra-articular fractures of the calcaneum were included. Their initial radiographs and CT films were blinded and assessed by three independent observers. Based on this they were selected for operative or non-operative management. The actual management was also recorded. The data were then subjected to statistical analysis to ...

  6. The results of conservative treatment of intra-articular fractures in the calcaneum

    OpenAIRE

    Arslan, Huseyin; Subasi, Mehmet; Kesemenli, Cumhur; Necmioglu, Serdar

    2004-01-01

    Objectives: We evaluated the results of conservative treatment for displaced intra-articular fractures of the calcaneum and investigated the implications of radiologic and clinical signs on the outcome. Methods: Thirty-three patients (18 males, 15 females; mean age 38 years; range 18 to 61 years) with displaced intra-articular calcaneum fractures were treated with cast immobilization and non-weight bearing. According to the modified Essex-Lopresti classification, the fractures were type-a ...

  7. Development of a Valid and Reliable Knee Articular Cartilage Condition–Specific Study Methodological Quality Score

    OpenAIRE

    Joshua D Harris; Erickson, Brandon J.; Cvetanovich, Gregory L.; Abrams, Geoffrey D.; McCormick, Frank M.; Gupta, Anil K.; Nikhil N. Verma; Bach, Bernard R.; Cole, Brian J.

    2014-01-01

    Background: Condition-specific questionnaires are important components in evaluation of outcomes of surgical interventions. No condition-specific study methodological quality questionnaire exists for evaluation of outcomes of articular cartilage surgery in the knee. Purpose: To develop a reliable and valid knee articular cartilage–specific study methodological quality questionnaire. Study Design: Cross-sectional study. Methods: A stepwise, a priori–designed framework was created for developme...

  8. Can intra-articular therapies prior to total knee arthroplasty increase the risk of periprosthetic infection?

    Science.gov (United States)

    Yeo, Q Y; Lye, D C; Sathappan Ss, S S

    2015-02-01

    Intra-articular therapies, such as steroid injection, viscosupplement injection and acupuncture, are common non-surgical options for patients with osteoarthritis of the knee. With any intra-articular injection or acupuncture procedure, there is a potential for inoculation with bacteria leading to possible knee infection. The authors report a patient who incurred an acute infection found after a total knee arthroplasty attributed to prior acupuncture procedure done as part of conservative treatment.

  9. The Biological Effects of Sex Hormones on Rabbit Articular Chondrocytes from Different Genders

    OpenAIRE

    Shwu Jen Chang; Shyh Ming Kuo; Yen Ting Lin; Shan-Wei Yang

    2014-01-01

    The aim of this study was to investigate the biological effects of sex hormones (17 β -estradiol and testosterone) on rabbit articular chondrocytes from different genders. We cultured primary rabbit articular chondrocytes from both genders with varying concentration of sex hormones. We evaluate cell proliferation and biochemical functions by MTT and GAG assay. The chondrocyte function and phenotypes were analyzed by mRNA level using RT-PCR. Immunocytochemical staining was also used to evaluat...

  10. Manifestações articulares em pacientes com doença de Crohn e retocolite ulcerativa Articular manifestations in patients with Crohn's disease and ulcerative colitis

    OpenAIRE

    Cristina Costa Duarte Lanna; Maria de Lourdes Abreu Ferrari; Marco Antônio Parreiras de Carvalho; Aloísio Sales da Cunha

    2006-01-01

    Os sintomas articulares constituem a manifestação extra-intestinal mais comum em pacientes com doença de Crohn e com retocolite ulcerativa. Reconhecida como artrite colítica, e mais recentemente, artrite enteropática, está classificada como uma das doenças do grupo das espondiloartropatias. Reconhecidamente, há dois padrões de acometimento articular: 1) periférico, habitualmente, associado aos períodos de atividade da doença intestinal e sem associação com o antígeno HLA B27, e 2) axial, cara...

  11. Diagnosis and Management of Extra-articular Causes of Pain After Total Knee Arthroplasty.

    Science.gov (United States)

    Manning, Blaine T; Lewis, Natasha; Tzeng, Tony H; Saleh, Jamal K; Potty, Anish G R; Dennis, Douglas A; Mihalko, William M; Goodman, Stuart B; Saleh, Khaled J

    2015-01-01

    Postoperative pain, which has been attributed to poor outcomes after total knee arthroplasty (TKA), remains problematic for many patients. Although the source of TKA pain can often be delineated, establishing a precise diagnosis can be challenging. It is often classified as intra-articular or extra-articular pain, depending on etiology. After intra-articular causes, such as instability, aseptic loosening, infection, or osteolysis, have been ruled out, extra-articular sources of pain should be considered. Physical examination of the other joints may reveal sources of localized knee pain, including diseases of the spine, hip, foot, and ankle. Additional extra-articular pathologies that have potential to instigate pain after TKA include vascular pathologies, tendinitis, bursitis, and iliotibial band friction syndrome. Patients with medical comorbidities, such as metabolic bone disease and psychological illness, may also experience prolonged postoperative pain. By better understanding the diagnosis and treatment options for extra-articular causes of pain after TKA, orthopaedic surgeons may better treat patients with this potentially debilitating complication. PMID:25745922

  12. T2 star relaxation times for assessment of articular cartilage at 3 T: a feasibility study

    Energy Technology Data Exchange (ETDEWEB)

    Mamisch, Tallal Charles [University Bern, Department of Orthopedic Surgery, Inselspital, Bern (Switzerland); University Bern, Magnetic Resonance Spectroscopy and Methodology, Department of Clinical Research, Bern (Switzerland); Hughes, Timothy [Siemens Medical Solutions, Erlangen (Germany); Mosher, Timothy J. [Penn State University College of Medicine, Musculoskeletal Imaging and MRI, Department of Radiology, Hershey, PA (United States); Mueller, Christoph [University of Erlangen, Department of Trauma Surgery, Erlangen (Germany); Trattnig, Siegfried [Medical University of Vienna, MR Center - High Field MR, Department of Radiology, Vienna (Austria); Boesch, Chris [University Bern, Magnetic Resonance Spectroscopy and Methodology, Department of Clinical Research, Bern (Switzerland); Welsch, Goetz Hannes [University of Erlangen, Department of Trauma Surgery, Erlangen (Germany); Medical University of Vienna, MR Center - High Field MR, Department of Radiology, Vienna (Austria)

    2012-03-15

    T2 mapping techniques use the relaxation constant as an indirect marker of cartilage structure, and the relaxation constant has also been shown to be a sensitive parameter for cartilage evaluation. As a possible additional robust biomarker, T2* relaxation time is a potential, clinically feasible parameter for the biochemical evaluation of articular cartilage. The knees of 15 healthy volunteers and 15 patients after microfracture therapy (MFX) were evaluated with a multi-echo spin-echo T2 mapping technique and a multi-echo gradient-echo T2* mapping sequence at 3.0 Tesla MRI. Inline maps, using a log-linear least squares fitting method, were assessed with respect to the zonal dependency of T2 and T2* relaxation for the deep and superficial regions of healthy articular cartilage and cartilage repair tissue. There was a statistically significant correlation between T2 and T2* values. Both parameters demonstrated similar spatial dependency, with longer values measured toward the articular surface for healthy articular cartilage. No spatial variation was observed for cartilage repair tissue after MFX. Within this feasibility study, both T2 and T2* relaxation parameters demonstrated a similar response in the assessment of articular cartilage and cartilage repair tissue. The potential advantages of T2*-mapping of cartilage include faster imaging times and the opportunity for 3D acquisitions, thereby providing greater spatial resolution and complete coverage of the articular surface. (orig.)

  13. Anticuerpo anticitrulina y manifestaciones extra articulares en artritis reumatoidea

    Directory of Open Access Journals (Sweden)

    María Jezabel Haye Salinas

    2013-02-01

    Full Text Available Los pacientes con artritis reumatidea (AR pueden desarrollar manifestaciones extra articulares (MExA, relacionadas a su morbi-mortalidad. Los anticuerpos anti-péptidos citrulinados cíclicos (ACCP son específicos para la AR y estan relacionados con el daño articular; y podrían tener rol patogénico en las MExA. Nuestro objetivo fue determinar la relación entre los anticuerpos ACCP y MExA en pacientes con AR. Se incluyeron 74 pacientes con diagnóstico de AR (ACR 1987 mayores de 18 años, de más de 6 meses de evolución, con MExA, y un control apareado por sexo y edad sin MExA por cada paciente. Las variables demográficas, clínicas y de laboratorio se compararon con test t, chi cuadrado o Mann-Whitney. Se realizó análisis multivariado; p ≤ 0.05. Los pacientes con MExA presentaron mayor título de anticuerpo ACCP (116 vs. 34, p < 0.01 y de factor reumatoideo (FR (108 vs. 34.5, p < 0.01. En el análisis multivariado hubo asociación entre la presencia de MExA y tabaquismo activo (p = 0.02, OR: 3.78, IC 95%: 1.17-12.2, FR positivo (p = 0.04, OR: 3.23, IC95%: 1.04-11.8 y anticuerpo ACCP positivo (p = 0.04, OR: 3.23, IC 95%: 1.04-10. Presentaron mayor título de anticuerpo ACCP que los controles los pacientes con xerostomía (109 vs. 34, p = 0.04, xeroftalmia (150 vs. 34, p < 0.01, nódulos sub-cutáneos (NSC (141 vs. 34, p < 0.01 y fibrosis pulmonar (158 vs. 34, p = 0.04. En conclusión, el anticuerpo ACCP positivo, el FR positivo y el tabaquismo activo fueron factores de riesgo independientes para el desarrollo de MExA.

  14. Infección Activa por el Citomegalovirus (CMV) en el trasplante alogénico de precursores hematopoyéticos:Investigación de nuevos factores biológicos para la estimación del riesgo y análisis integral de los mecanismos inmunitarios que la previenen y controlan

    OpenAIRE

    Giménez Quiles, Estela Berenice

    2016-01-01

    Citomegalovirus (CMV) es causa frecuente de morbididad y mortalidad en el marco del trasplante alogénico de precursores hematopoyéticos (alo-TPH). CMV puede ocasionar enfermedad orgánica o tisular (neumonía intersticial o enfermedad gastrointestinal, con mayor frecuencia), en virtud de su “citopatogenicidad” . CMV también puede causar morbididad a través de mecanismos indirectos, relacionados con su capacidad inmunosupresora y pro-inflamatoria; en ese contexto, CMV parece incrementar el ries...

  15. Análisis de polimorfismos de genes implicados en la regulación del endotelio y la inflamación en pacientes con insuficiencia cardíaca y su relación con el tipo de disfunción ventricular predominante

    OpenAIRE

    Ruiz Olgado, Maria Jose

    2010-01-01

    [ES] La insuficiencia cardiaca está determinada por factores genéticos y ambientales. El sistema endotelial y la respuesta inflamatoria son esenciales en el remodelado vascular en la insuficiencia cardiaca. Polimorfismos de genes que codifican la endotelina-1 (ET-1), la sintasa endotelial del óxido nítrico (NOS), la interleucina-6 (IL-6) y la ciclooxigenasa-2 (COX2) podrían estar relacionados con el remodelado ventricular en pacientes con insuficiencia cardiaca. Nuestro objetivo fue invest...

  16. Anticuerpo anticitrulina y manifestaciones extra articulares en artritis reumatoidea Anticitrulin antibody and the extra-articular manifestations in rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    María Jezabel Haye Salinas

    2013-02-01

    Full Text Available Los pacientes con artritis reumatidea (AR pueden desarrollar manifestaciones extra articulares (MExA, relacionadas a su morbi-mortalidad. Los anticuerpos anti-péptidos citrulinados cíclicos (ACCP son específicos para la AR y estan relacionados con el daño articular; y podrían tener rol patogénico en las MExA. Nuestro objetivo fue determinar la relación entre los anticuerpos ACCP y MExA en pacientes con AR. Se incluyeron 74 pacientes con diagnóstico de AR (ACR 1987 mayores de 18 años, de más de 6 meses de evolución, con MExA, y un control apareado por sexo y edad sin MExA por cada paciente. Las variables demográficas, clínicas y de laboratorio se compararon con test t, chi cuadrado o Mann-Whitney. Se realizó análisis multivariado; p ≤ 0.05. Los pacientes con MExA presentaron mayor título de anticuerpo ACCP (116 vs. 34, p A large proportion of rheumatoid arthritis (RA patients develop extra-articular manifestations (EAM, which are associated with morbidity and early mortality. Anti cyclic citrullinated peptide (ACCP antibody has proven to be highly specific for the diagnosis of RA, associated with severe joint damage and may have some role in the pathogenesis of EAM. The aim of this study was to determine the relationship between ACCP antibody and the presence of EAM in RA patients. Seventy four RA patients (ACR 1987 with EAM, > 18 years, more than 6 months duration were included, and an EAM free control, matched by sex and age, for each patient. Demographic, clinical and laboratory variables were compared using t-test, chi-square or Mann-Whitney test. Multivariate analysis was performed: p ≤ 0.05. Patients with EAM presented a greater value of ACCP antibody (116 vs. 34, p < 0.01 and rheumatoid factor (108 vs. 34.5, p < 0.01. Independent association with current smoking habit (p = 0.02, OR = 3.78, 95%: 1.17-12.2, RF positive (p = 0.04, OR 3.23, CI 95%: 1.04 to 11.8 and ACCP antibody positive (p = 0.04, OR 3.23, 95% CI: 1

  17. Early Intra-Articular Complement Activation in Ankle Fractures

    Directory of Open Access Journals (Sweden)

    Hagen Schmal

    2014-01-01

    Full Text Available Cytokine regulation possibly influences long term outcome following ankle fractures, but little is known about synovial fracture biochemistry. Eight patients with an ankle dislocation fracture were included in a prospective case series and matched with patients suffering from grade 2 osteochondritis dissecans (OCD of the ankle. All fractures needed external fixation during which joint effusions were collected. Fluid analysis was done by ELISA measuring aggrecan, bFGF, IL-1β, IGF-1, and the complement components C3a, C5a, and C5b-9. The time periods between occurrence of fracture and collection of effusion were only significantly associated with synovial aggrecan and C5b-9 levels (P<0.001. Furthermore, synovial expressions of both proteins correlated with each other (P<0.001. Although IL-1β expression was relatively low, intra-articular levels correlated with C5a (P<0.01 and serological C-reactive protein concentrations 2 days after surgery (P<0.05. Joint effusions were initially dominated by neutrophils, but the portion of monocytes constantly increased reaching 50% at day 6 after fracture (P<0.02. Whereas aggrecan and IL-1β concentrations were not different in fracture and OCD patients, bFGF, IGF-1, and all complement components were significantly higher concentrated in ankle joints with fractures (P<0.01. Complement activation and inflammatory cell infiltration characterize the joint biology following acute ankle fractures.

  18. Hydrogels as a Replacement Material for Damaged Articular Hyaline Cartilage

    Directory of Open Access Journals (Sweden)

    Charlotte M. Beddoes

    2016-06-01

    Full Text Available Hyaline cartilage is a strong durable material that lubricates joint movement. Due to its avascular structure, cartilage has a poor self-healing ability, thus, a challenge in joint recovery. When severely damaged, cartilage may need to be replaced. However, currently we are unable to replicate the hyaline cartilage, and as such, alternative materials with considerably different properties are used. This results in undesirable side effects, including inadequate lubrication, wear debris, wear of the opposing articular cartilage, and weakening of the surrounding tissue. With the number of surgeries for cartilage repair increasing, a need for materials that can better mimic cartilage, and support the surrounding material in its typical function, is becoming evident. Here, we present a brief overview of the structure and properties of the hyaline cartilage and the current methods for cartilage repair. We then highlight some of the alternative materials under development as potential methods of repair; this is followed by an overview of the development of tough hydrogels. In particular, double network (DN hydrogels are a promising replacement material, with continually improving physical properties. These hydrogels are coming closer to replicating the strength and toughness of the hyaline cartilage, while offering excellent lubrication. We conclude by highlighting several different methods of integrating replacement materials with the native joint to ensure stability and optimal behaviour.

  19. Rice Body Formation Within a Peri-Articular Shoulder Mass.

    Science.gov (United States)

    Edison, Michele N; Caram, Anthony; Flores, Miguel; Scherer, Kurt

    2016-01-01

    Most commonly associated with chronic inflammatory conditions, rice bodies represent an uncommon, nonspecific, often intra-articular inflammatory process. Presumably, rice bodies represent the sequelae of microvascular infarcts of the joint synovium. However, rice bodies have been seen in pleural fluid, in the setting of bursitis, and within the tendon sheath. The etiology and prognostic significance of rice bodies are not clear. MRI is the diagnostic imaging modality of choice for the evaluation of rice body formation. Here we present a case of a 28-year-old female with a history of rheumatoid arthritis (RA) who presented to her primary care physician with a palpable mass around her right shoulder which was presumed to be a lipoma. An initial ultrasound showed a fluid filled structure with internal debris. Subsequent MRI evaluation was confirmatory for subacromial-subdeltoid bursitis with rice body formation. The salient point of this report is to highlight the importance of patient-specific differential diagnosis. While lipomas are a very common benign soft tissue tumor, patients with RA often have disease-specific sequelae that should be included in the diagnostic deliberation. Thus, when ordering diagnostic testing for patients with a palpable mass and rheumatoid arthritis, MRI--possibly preceded by conventional radiography--is the most appropriate diagnostic algorithm. PMID:27625904

  20. Spectrocolorimetric evaluation of repaired articular cartilage after a microfracture

    Directory of Open Access Journals (Sweden)

    Dohi Yoshihiro

    2008-09-01

    Full Text Available Abstract Background In clinical practice, surgeons differentiate color changes in repaired cartilage compared with surrounding intact cartilage, but cannot quantify these color changes. Objective assessments are required. A spectrocolorimeter was used to evaluate whether intact and repaired cartilage can be quantified. Findings We investigated the use of a spectrocolorimeter and the application of two color models (L* a* b* colorimetric system and spectral reflectance distribution to describe and quantify articular cartilage. In this study, we measured the colors of intact and repaired cartilage after a microfracture. Histologically, the repaired cartilage was a mixture of fibrocartilage and hyaline cartilage. In the L* a* b* colorimetric system, the L* and a* values recovered to close to the values of intact cartilage, whereas the b* value decreased over time after the operation. Regarding the spectral reflectance distribution at 12 weeks after the operation, the repaired cartilage had a higher spectral reflectance ratio than intact cartilage between wavelengths of 400 to 470 nm. Conclusion This study reports the first results regarding the relationship between spectrocolorimetric evaluation and the histological findings of repair cartilage after a microfracture. Our findings demonstrate the ability of spectrocolorimetric measurement to judge the repair cartilage after treatment on the basis of objective data such as the L*, a* and b* values and the SRP as a coincidence index of the spectral reflectance curve.

  1. Extra-articular manifestations of rheumatoid arthritis: An update.

    Science.gov (United States)

    Prete, Marcella; Racanelli, Vito; Digiglio, Liboria; Vacca, Angelo; Dammacco, Franco; Perosa, Federico

    2011-12-01

    Rheumatoid arthritis (RA) is an immune-mediated disease involving chronic low-grade inflammation that may progressively lead to joint destruction, deformity, disability and even death. Despite its predominant osteoarticular and periarticular manifestations, RA is a systemic disease often associated with cutaneous and organ-specific extra-articular manifestations (EAM). Despite the fact that EAM have been studied in numerous RA cohorts, there is no uniformity in their definition or classification. This paper reviews current knowledge about EAM in terms of frequency, clinical aspects and current therapeutic approaches. In an initial attempt at a classification, we separated EAM from RA co-morbidities and from general, constitutional manifestations of systemic inflammation. Moreover, we distinguished EAM into cutaneous and visceral forms, both severe and not severe. In aggregated data from 12 large RA cohorts, patients with EAM, especially the severe forms, were found to have greater co-morbidity and mortality than patients without EAM. Understanding the complexity of EAM and their management remains a challenge for clinicians, especially since the effectiveness of drug therapy on EAM has not been systematically evaluated in randomized clinical trials. PMID:21939785

  2. Intracortical chondroblastoma mimicking intra-articular osteoid osteoma

    Energy Technology Data Exchange (ETDEWEB)

    Ishida, Tsuyoshi; Mukai, Kiyoshi [First Department of Pathology, Tokyo Medical University, Shinjuku 6-1-1, Shinjuku-ku, Tokyo 160-8402 (Japan); Goto, Takahiro [Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo, Tokyo (Japan); Motoi, Noriko [Department of Pathology, Toranomon Hospital, Tokyo (Japan)

    2002-10-01

    We report a case of intra-articular intracortical chondroblastoma of the femoral condyle which radiologically appeared to be osteoid osteoma. A 19-year-old woman presented with a 3-year history of gradually increasing pain in the right knee and had been on nonsteroidal anti-inflammatory drugs for pain relief. Laboratory data were within normal limits. Radiographs showed a well-demarcated lucent lesion in the medial condyle of the right femur. A nidus-like lesion with calcifications and a sclerotic rim located in the cortex was imaged by computed tomography scan. Magnetic resonance imaging revealed bone marrow edema and soft tissue swelling around the lesion, with low signal intensity of the nidus-like lesion on both T1- and T2-weighted images. The lesion was excised en bloc and the histological diagnosis of chondroblastoma was made. A mild inflammatory reaction was observed in the bone marrow and synovium around the tumor. The chondroblastoma cells were shown to express cyclooxygenase-2 with immunohistochemistry. (orig.)

  3. ACTIVITY OF CANONICAL WNT SIGNAL SYSTEM IN HYALINE CARTILAGE ARTICULAR CHONDROCYTES IN PROCESS OF SYNOVIAL JOINT DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    A.O. Molotkov

    2009-03-01

    Full Text Available Canonical and non-canonical Wnt systems are essential regulators of chondrogenesis and bone development. However, the roles of these systems in synovial joint development are not well studied. To determine if canonical Wnt system is active in developing articular chondrocytes we used immunohistochemistry for в-galactosidase and doublecortin (cell-type specific marker for articular chondrocytes to double label sections through joint regions of E14.5, E18.5, P10 and adult mice. Here the following results are presented. Canonical Wnt signal system does not work in developing articular chondrocytes at early embryonic stages (E14.5; it is active in the articular chondrocytes at late embryonic stages (E16.5-E18.5 and during postnatal development (P7-P10, but is turned off again in the adult articular chondrocytes. These results suggest that canonical Wnt signaling is being regulated during articular chondrocytes differentiation and joint formation.

  4. Asociación entre porphyromona gingivalis y proteína C reactiva en enfermedades sistémicas inflamatorias Association between porphyromonas gingivalis and C-reactive protein in systemic inflammatory diseases

    Directory of Open Access Journals (Sweden)

    C.M. Ardila Medina

    2010-04-01

    Full Text Available La proteína C reactiva (PCR es un marcador serológico de la inflamación asociado con incremento en el riesgo de enfermedades sistémicas inflamatorias (ESI. La periodontitis también se relaciona con niveles elevados de PCR en adultos y con una reducción de la misma después de su tratamiento. Así, se ha postulado que la PCR puede ser un posible mediador de la asociación entre periodontitis y ESI. Los patógenos periodontales además de inducir inflamación local y destrucción tisular están involucrados en el aumento de la respuesta sistémica inflamatoria e inmunológica. Diferentes autores han investigado la relación entre los anticuerpos para algunos patógenos periodontales y la PCR, pero la asociación se ha notificado firmemente para IgG a Porphyromona gingivalis. Es escasa la evidencia de asociación de una medida directa entre patógenos periodontales y PCR, sin embargo es muy importante debido a que la presencia de anticuerpos no necesariamente es un indicador de infección activa.C-reactive protein (CRP is a serological marker of systemic inflammation that has been associated with increased risk systemic inflammatory diseases. Periodontitis has also been linked to elevated CRP levels in adults as well as with a reduction in PCR after its treatment. It is thus postulated that CRP might be a possible mediator of the association between periodontitis and systemic inflammatory diseases. Periodontal pathogens do not induce only local inflammation and tissue destruction. They are also involved in systemic increases in inflammatory and inmmune responses. Several studies have investigated antibodies to various periodontal pathogens in relation to CRP, but the association has been reported consistently only for IgG to Porphyromonas gingivalis. Evidence is sparse on the association between a direct measure of periodontal pathogens and CRP, while it is more important because the presence of antibody titers is not necessarily indicative

  5. Efecto de las cargas articulares sobre el flujo y pH salival Effect of TMJ loading on salivary flow and pH

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    RJ Castro

    2011-04-01

    Full Text Available Una alteración del flujo salival es clave en el desarrollo de caries, enfermedad periodontal e infecciones oportunistas. El flujo salival está determinado por diversos estímulos que actúan sobre receptores de distinta naturaleza, entre ellos mecanoreceptores articulares. Algunos estudios demuestran que las cargas articulares actúan sobre estos receptores, modificando cualitativa y cuantitativamente la secreción salival. El objetivo de este estudio fue determinar si existe una relación entre la aplicación controlada de cargas articulares y cambios en el flujo y pH salival. 30 voluntarios fueron seleccionados según criterios de inclusión establecidos. Se elaboró un dispositivo interoclusal que en relación a piezas 1.5 y 2.5, tenía botones acrílicos de 1 mm de espesor, los que al ejercer fuerzas sobre ellos provocan cargas reversibles sobre la ATM. Antes de usar el dispositivo, en cada individuo se midió flujo salival no estimulado (FSNE el flujo salival estimulado (FSE y el pH de ambos. Tras un periodo de adaptación diaria por una semana, cada participante usó el dispositivo durante 1 hora, ejerciendo fuerza masticatoria sostenida. Luego se midieron ambos flujos y el pH correspondiente. El procedimiento fue repetido por 7 días, bajo supervisión. No se observó una relación aparente entre la aplicación controlada de cargas articulares y cambios volumétricos de flujo salival. Las mujeres tuvieron un FSE y FSNE menor al de los hombres. La aplicación de sobrecargas articulares por un breve período de tiempo provoca una caída del pH del FSE luego de 3 días de aplicación.Altered salivary flow is key for the onset of dental caries, periodontal disease and opportunistic infections. Salivary flow is determined by various stimuli on several receptors, including joint mechanoreceptors. Some studies show that loads on the TMJ affect these receptors causing changes on the quality and quantity of saliva secretion. The aim of this

  6. Assessment of Intraoperative Intra-articular Morphine and Clonidine Injection in the Acute Postoperative Period After Hip Arthroscopy

    OpenAIRE

    Cogan, Charles J.; Knesek, Michael; Tjong, Vehniah K.; Nair, Rueben; Kahlenberg, Cynthia; Dunne, Kevin F.; Mark C. Kendall; Terry, Michael A.

    2016-01-01

    Background: Previous authors have suggested that intra-articular morphine and clonidine injections after knee arthroscopy have demonstrated equivocal analgesic effect in comparison with bupivacaine while circumventing the issue of chondrotoxicity. There have been no studies evaluating the effect of intra-articular morphine after hip arthroscopy. Purpose: To evaluate the efficacy of intra-articular morphine in combination with clonidine on postoperative pain and narcotic consumption after hip ...

  7. Inestabilidad compleja del codo

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    Héctor Gabriel Díaz Carrillo

    2014-08-01

    Full Text Available La inestabilidad compleja del codo es de reciente descripción y controversial en sus resultados; es el estado que se deriva del daño estructural y funcional, producido por la incongruencia de las superficies articulares y las estructuras ligamentosas que estabilizan el codo. Se realizó un estudio descriptivo prospectivo, en el período comprendido entre enero de 2011 y abril de 2013, en una muestra no probabilística de ocho pacientes (diez codos, seleccionados según criterios de inclusión y exclusión. Los objetivos fundamentales del estudio fueron: la actualización de conocimientos para el manejo de la enfermedad y la caracterización de los resultados obtenidos en los afectados por esta dolencia que fueron operados en el Hospital Dr. Ernesto Guevara de la Serna de la provincia de Las Tunas. Se analizaron los datos mediante técnicas estadísticas descriptivas y se realizó la evaluación clínica y radiográfica de los lesionados. El tratamiento se ejecutó según los hallazgos y consistió en: reparación ligamentosa, actuación sobre las partes óseas dañadas y colocación de osteosíntesis diversas. La edad promedio fue de 39,2 años y predominó el sexo masculino. A todos se les practicó reparación ligamentosa, siete cupulectomías y seis aplicaciones de cerclajes anti-distractores. Existieron dos reluxaciones y una sepsis local. La amplitud de movimientos fue aceptable dentro del rango compatible, con una buena función ergonómica; de acuerdo a las comparaciones bibliográficas actualizadas que se realizaron. La fijación y reparación de los estabilizadores del codo brindan buenos resultados funcionales. La sistematización en el manejo de la enfermedad, la detección de complicaciones y el arsenal terapéutico disponible son factores a tener en cuenta

  8. Avaliação microbiológica e molecular de líquidos articulares e peri-articulares de suínos

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    Ana Carolina S. Faria

    2011-08-01

    Full Text Available No presente estudo coletaram-se 115 amostras de líquido articular e peri-articular de suínos com suspeita clínica de doença articular oriundos de maternidade (30,43%, creche (44,35% e crescimento/terminação (25,22% de Sistemas Intensivos de Produção de Suínos (SIPs para avaliação microbiológica e molecular. Observaram-se 57 (49,5% amostras positivas em pelo menos uma das técnicas. No isolamento microbiano, 39,13% das amostras foram positivas, sendo Streptococcus spp. (19,72%, Arcabobacterium pyogenes (18,13% e Escherichia coli (12,68% os mais frequentes, havendo também a presença de Candida sp. (2,6%. Na técnica de Reação em Cadeia da Polimerase (PCR, em 20% das amostras foram detectados microrganismos com uma maior ocorrência de Mycoplasma hyosinoviae (34,09%, Erysipelotrix tonsilarum (20,45% e Haemophilus parasuis (15,90%. Os microrganismos mais frequentemente isolados em animais com artrite, apresentaram distribuição em todas as faixas etárias, entretanto a fase de crescimento/terminação apresentou maior percentual (69% de amostras positivas. Streptococcus spp. ocorreu em todas as fases sendo o microrganismo mais detectado. M. hyosinoviae foi observado principalmente em animais de creche. Na fase de crescimento/terminação as bactérias predominantes foram A. pyogenes, H. parasuis e E. tonsilarum. Aproximadamente metade dos casos foi negativo o que indica a provável ocorrência de processos degenerativos como a osteocondrose, embora a participação de infecções articulares e peri-articulares possam representar grandes perdas com menor ou maior impacto dependendo da fase de criação. Problemas articulares e/ou peri-articulares de origem infecciosas foram encontrados em todas as propriedades estudadas. O principal agente foi M. hyosynoviae, principalmente na creche, porém não se pode descartar o envolvimento de problemas degenerativos em associação.

  9. Usefulness of fluoroscopy-guided intra-articular injection of the knee

    Energy Technology Data Exchange (ETDEWEB)

    Myung, Jae Sung; Lee, Joon Woo [Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Lee, Ji Yeon [Kangwon National University College of Medicine, Chuncheon (Korea, Republic of)] (and others)

    2007-06-15

    To determine the accuracy of the intra-articular location of hyaluronic acid injection using a blind approach and to establish the usefulness of fluoroscopy-guided intra-articular injection. A fluoroscopy unit was used for 368 intra-articular injections of hyaluronic acid to 93 knees in 65 patients. Initially, blind needle positioning was conducted on the fluoroscopy table. The failure rate of the blind approach among the 368 injections was evaluated, and a relationship between the Kellgren-Lawrence grade (K-L grade) and the incidence of repeated failures using the blind approach was determined for injections to 52 knees in 37 patients who received a complete cycle of injections (five consecutive injections with a one-week interval between injections). Using a blind approach, 298 of 368 trials (81.2%) resulted in a needle tip being placed in an intra-articular location, while 70 of 368 trials resulted in an extra-articular placement of the needle tip. Among 52 knees to which a complete cycle of injection (five consecutive injections with a one-week interval between injections) was administered, repeated failure of intra-articular placement using the blind approach was seen for 18 knees (34.6%); a more severe K-L grade assigned was associated with a higher rate of repeated failure. However, the trend was not statistically significant based on the Chi-squared test ({rho} value = 0.14). Fluoroscopy-guided needle placement may be helpful to ensure therapeutic intra-articular injection of the knee.

  10. OPERATIVE MANAGEMENT OF INTRA-ARTICULAR DISTAL HUMERAL FRACTURES WITH LOCKING PLATES

    Directory of Open Access Journals (Sweden)

    Abhilekh

    2015-11-01

    Full Text Available BACKGROUND: Intra-articular distal humeral fractures are common, but complex elbow injuries. To obtain good results, anatomical reduction with rigid fixation and early range of mobilization is required. Treatment of these fractures with conventional plates is associated with many complications such as non-anatomic reduction of articular surfaces, malunion, non-union, loosening of implant, residual stiffness of the elbow and post-traumatic osteoarthrosis. In this situation the application of locking plates having a fixed angle plate screw construct can minimise most of the above complications. OBJECTIVE: To evaluate radiological and functional outcome of locking plate application for the management of intra-articular distal humeral fractures. MATERIAL AND METHODS: This prospective study was conducted from January 2013 to December 2014. We operated 20 patients of AO type-C intra-articular distal humeral fractures. Fracture was exposed using modified Campbell’s posterior approach in less comminuted fractures and a V-shaped Olecranon osteotomy was done to get better exposure of the articular surface in cases with severe articular comminution. The fracture was stabilized using an intercondylar screw, pre-contoured locking compression plates and/or locking reconstruction plates as per preoperative planning. Patients were reviewed at monthly interval for clinical-radiological evaluation. Final outcome measures included radiological assessment, range of motion and Mayo elbow performance score (MEPS. RESULTS: All the fractures were united at an average 12 weeks. Two patients developed numbness in the distribution of ulnar nerve and one patient developed superficial infection in immediate postoperative period. None of the patients had malunion and loosening of implant. The average arc of flexion-extension was 105`, although no patient had loss of supination/pronation. Mayo Elbow Performance Score was excellent in 15 (75%, good in 3 (15%, fair in 1 (5

  11. Análisis de la reacción inflamatoria y los procesos degenerativos provocados por la inyección intracerebroventricular de neuraminidasa en rata

    OpenAIRE

    Granados-Durán, Pablo

    2014-01-01

    Las infecciones víricas y bacterianas del sistema nervioso central (SNC) y la neuroinflamación han sido relacionadas en los últimos años con en el desarrollo de ciertas enfermedades neurodegenerativas, tales como la esclerosis múltiple, la enfermedad de Alzheimer, la esclerosis lateral amiotrófica o trastornos neurocognitivos como la demencia asociada al VIH o la esquizofrenia. Muchas de estas enfermedades continúan hoy sin tratamiento, incluyendo aquellas que son sospechosas de ser consecuen...

  12. Patogénesis de la enfermedad pélvica inflamatoria. Modelo animal con la biovariedad murina de Chlamydia trachomatis

    Directory of Open Access Journals (Sweden)

    Fabio Carmona Velásquez

    2009-10-01

    Full Text Available Se inocularon hembras de ratones blancos suizo (Balb/c, entre 4 y 6 semanas con la serovariedad MoPn de Chlamydia trachomatis para producirles una salpingitis clamidial experimental. Directamente en la rama uterina izquierda se inyectaron 30 µl equivalente a 6 x 104 UFI. La misma cantidad se inyectó en la bursa del ovario del mismo lado, a través del tejido graso que se encuentra en este sitio. Se sacrificaron grupos de hembras a los 7 y 14 días después de la inoculación para buscar inflamación, hidrosálpinx y respuesta de anticuerpos tipo IgG e IgM. Otras hembras inoculadas se aparearon para observar la tasa de infertilidad bilateral que ocasionaba la infección. Un tercer grupo de animales se trató con tetraciclina, ibuprofén y prostaglandina E1. El tratamiento temprano, hacia el segundo día después de la inoculación, impidió la inflamación, la formación de hidrosálpinx y la infertilidad normal bilateral. Los agentes antiinflamatorios, usados solos, no tuvieron ningún efecto para prevenir la inflamación, ni aun en combinación con tetraciclina. Un cuarto grupo se trató con anticuerpos monoclonales dirigidos contra la subpoblación linfocítica L3T4 (equivalente en seres humanos a CD4. En los ratones con L3T4 se observó un agotamiento (depleción total de la población linfocítica L3T4 hacia el segundo día después de la inoculación. El agotamiento persistió por lo menos hasta el día 20, pero no alteró significativamente los porcentajes de células expresadas por Thy 1.2 (célula de antígeno T o Lyt2 (célula de antígeno T supresor/citotóxico, que equivale a los CD8 en el hombre. Se concluye que los continuos inóculos con Chlamydia, generan una respuesta inmune que termina con daño del tejido y conduce a la infertilidad.

  13. Nanosized fibers' effect on adult human articular chondrocytes behavior

    International Nuclear Information System (INIS)

    Tissue engineering with chondrogenic cell based therapies is an expanding field with the intention of treating cartilage defects. It has been suggested that scaffolds used in cartilage tissue engineering influence cellular behavior and thus the long-term clinical outcome. The objective of this study was to assess whether chondrocyte attachment, proliferation and post-expansion re-differentiation could be influenced by the size of the fibers presented to the cells in a scaffold. Polylactic acid (PLA) scaffolds with different fiber morphologies were produced, i.e. microfiber (MS) scaffolds as well as nanofiber-coated microfiber scaffold (NMS). Adult human articular chondrocytes were cultured in the scaffolds in vitro up to 28 days, and the resulting constructs were assessed histologically, immunohistochemically, and biochemically. Attachment of cells and serum proteins to the scaffolds was affected by the architecture. The results point toward nano-patterning onto the microfibers influencing proliferation of the chondrocytes, and the overall 3D environment having a greater influence on the re-differentiation. In the efforts of finding the optimal scaffold for cartilage tissue engineering, studies as the current contribute to the knowledge of how to affect and control chondrocytes behavior. - Highlights: ► Chondrocyte behavior in nanofiber-coated microfiber versus microfiber scaffolds ► High porosity (> 90%) and large pore sizes (a few hundred μm) of nanofibrous scaffolds ► Proliferation enhanced by presence of nanofibers ► Differentiation not significantly affected ► Cell attachment improved in presence of both nanofibers and serum

  14. Multi-parametric MRI characterization of enzymatically degraded articular cartilage.

    Science.gov (United States)

    Nissi, Mikko J; Salo, Elli-Noora; Tiitu, Virpi; Liimatainen, Timo; Michaeli, Shalom; Mangia, Silvia; Ellermann, Jutta; Nieminen, Miika T

    2016-07-01

    Several laboratory and rotating frame quantitative MRI parameters were evaluated and compared for detection of changes in articular cartilage following selective enzymatic digestion. Bovine osteochondral specimens were subjected to 44 h incubation in control medium or in collagenase or chondroitinase ABC to induce superficial collagen or proteoglycan (glycosaminoglycan) alterations. The samples were scanned at 9.4 T for T1 , T1 Gd (dGEMRIC), T2 , adiabatic T1 ρ , adiabatic T2 ρ , continuous-wave T1 ρ , TRAFF2 , and T1 sat relaxation times and for magnetization transfer ratio (MTR). For reference, glycosaminoglycan content, collagen fibril orientation and biomechanical properties were determined. Changes primarily in the superficial cartilage were noted after enzymatic degradation. Most of the studied parameters were sensitive to the destruction of collagen network, whereas glycosaminoglycan depletion was detected only by native T1 and T1 Gd relaxation time constants throughout the tissue and by MTR superficially. T1 , adiabatic T1 ρ , adiabatic T2 ρ , continuous-wave T1 ρ , and T1 sat correlated significantly with the biomechanical properties while T1 Gd correlated with glycosaminoglycan staining. The findings indicated that most of the studied MRI parameters were sensitive to both glycosaminoglycan content and collagen network integrity, with changes due to enzymatic treatment detected primarily in the superficial tissue. Strong correlation of T1 , adiabatic T1ρ , adiabatic T2 ρ , continuous-wave T1 ρ , and T1 sat with the altered biomechanical properties, reflects that these parameters were sensitive to critical functional properties of cartilage. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1111-1120, 2016. PMID:26662555

  15. Cartilage tissue engineering using pre-aggregated human articular chondrocytes

    Directory of Open Access Journals (Sweden)

    F Wolf

    2008-12-01

    Full Text Available In this study, we first aimed at determining whether human articular chondrocytes (HAC proliferate in aggregates in the presence of strong chondrocyte mitogens. We then investigated if the aggregated cells have an enhanced chondrogenic capacity as compared to cells cultured in monolayer. HAC from four donors were cultured in tissue culture dishes either untreated or coated with 1% agarose in the presence of TGFb-1, FGF-2 and PDGF-BB. Proliferation and stage of differentiation were assessed by measuring respectively DNA contents and type II collagen mRNA. Expanded cells were induced to differentiate in pellets or in Hyaff®-11 meshes and the formed tissues were analysed biochemically for glycosaminoglycans (GAG and DNA, and histologically by Safranin O staining. The amount of DNA in aggregate cultures increased significantly from day 2 to day 6 (by 3.2-fold, but did not further increase with additional culture time. Expression of type II collagen mRNA was about two orders of magnitude higher in aggregated HAC as compared to monolayer expanded cells. Pellets generated by aggregated HAC were generally more intensely stained for GAG than those generated by monolayer-expanded cells. Scaffolds seeded with aggregates accumulated more GAG (1.3-fold than scaffolds seeded with monolayer expanded HAC. In conclusion, this study showed that HAC culture in aggregates does not support a relevant degree of expansion. However, aggregation of expanded HAC prior to loading into a porous scaffold enhances the quality of the resulting tissues and could thus be introduced as an intermediate culture phase in the manufacture of engineered cartilage grafts.

  16. Arthroscopic laser in intra-articular knee cartilage disorders

    Science.gov (United States)

    Nosir, Hany R.; Siebert, Werner E.

    1996-12-01

    Different assemblies have endeavored to develop arthroscopic laser surgery. Various lasers have been tried in the treatment of orthopaedic problems, and the most useful has turned out to be the Hol-YAG laser 2.1 nm which is a near- contact laser. By using the laser as a powerful tool, and cutting back on the power level, one is able to better achieve the desired treatment effect. Clinical studies to evaluating the role of the laser in different arthroscopic knee procedures, comparing to conventional techniques, showed that the overall outcome attains a momentous confidence level which is shifted to the side of the laser versus the conventional for all maneuvers, barring meniscectomy where there is not perceiving disparity between laser versus the conventional. Meniscectomy continues to be one of the most commonly performed orthopaedic procedures. Laser provides a single tool which can ablate and debride meniscal rims with efficiency and safety. Chondroplasty can also be accomplished with ease using defocused laser energy. Both lateral release and soft tissue cermilization benefit from the cutting effect of laser along with its hemostatic effect. Synovial reduction with a defocused laser is also easily accomplished. By one gadget, one can cut, ablate, smooth, coagulate, congeal and with authentic tissue depth control The future of laser arthroscopic surgery lies in its ability to weld or repair tissues. Our research study has shown that laser activated photoactive dyes can produce a molecular bonding of collagen fibers, and therefore a repair 'weld' can be achieved with both meniscal tissues and with articular cartilage lesions.

  17. Serum-free media for articular chondrocytes in vitro expansion

    Institute of Scientific and Technical Information of China (English)

    SHAO Xin-xin; Neil A.Duncan; LIN Lin; FU Xin; ZHANG Ji-ying; YU Chang-long

    2013-01-01

    Background In vitro chondrocyte expansion is a major challenge in cell-based therapy for human articular cartilage repair.Classical culture conditions usually use animal serum as a medium supplement,which raises a number of undesirable questions.In the present study,two kinds of defined,serum-free media were developed to expand chondrocytes in monolayer culture for the purpose of cartilage tissue engineering.Methods Bovine chondrocytes were expanded in serum-free media supplemented with fibroblast growth factor-2 and platelet-derived growth factor or fibroblast growth factor-2 and insulin-like growth factor.Expansion culture in a conventional 10% fetal bovine serum (FBS) medium served as control.Fibronectin coating was used to help cell adhesion in serum-free medium.Next,in vitro three-dimensional pellet culture was used to evaluate the chondrocyte capacity.Cell pellets were expanded in different media to re-express the differentiated phenotype (re-differentiation) and to form cartilaginous tissue.The pellets were assessed by glycosaminoglycans contents,collagen II,collagen I and collagen X immunohistological staining.Results Chondrocytes cultured in serum-free media showed no proliferation difference than cells grown with 10% FBS medium.In addition,chondrocytes expanded in both serum-free media expressed more differentiated phenotypes at the end of monolayer culture,as indicated by higher gene expression ratios of collagen type Ⅱ to collagen type Ⅰ.Pellets derived from chondrocytes cultured in both serum-free media displayed comparable chondrogenic capacities to pellets from cells expanded in 10% FBS medium.Conclusion These findings provide alternative culture approaches for chondrocytes in vitro expansion,which may benefit the clinical use of autologous chondrocytes implantation.

  18. Role of computer aided detection (CAD) integration: case study with meniscal and articular cartilage CAD applications

    Science.gov (United States)

    Safdar, Nabile; Ramakrishna, Bharath; Saiprasad, Ganesh; Siddiqui, Khan; Siegel, Eliot

    2008-03-01

    Knee-related injuries involving the meniscal or articular cartilage are common and require accurate diagnosis and surgical intervention when appropriate. With proper techniques and experience, confidence in detection of meniscal tears and articular cartilage abnormalities can be quite high. However, for radiologists without musculoskeletal training, diagnosis of such abnormalities can be challenging. In this paper, the potential of improving diagnosis through integration of computer-aided detection (CAD) algorithms for automatic detection of meniscal tears and articular cartilage injuries of the knees is studied. An integrated approach in which the results of algorithms evaluating either meniscal tears or articular cartilage injuries provide feedback to each other is believed to improve the diagnostic accuracy of the individual CAD algorithms due to the known association between abnormalities in these distinct anatomic structures. The correlation between meniscal tears and articular cartilage injuries is exploited to improve the final diagnostic results of the individual algorithms. Preliminary results from the integrated application are encouraging and more comprehensive tests are being planned.

  19. Lipoma arborescens arising in the extra-articular bursa of the knee joint

    Directory of Open Access Journals (Sweden)

    Minami Shinji

    2016-01-01

    Full Text Available Lipoma arborescens arising in the extra-articular bursa of the knee joint is extremely rare. We describe an 11-year-old boy who complained of a gradual swelling mass of the lateral knee joint. Magnetic resonance imaging (MRI showed a high signal intensity tumor on T1- and T2-weighted images with a thickened septa and nodular lesion that showed low signal intensity. The radiologist suggested the possible differential diagnosis of well-differentiated liposarcoma. At operation, the tumor was found under the iliotibial tract and was not in contact with the knee joint. Histopathologically, this lesion was diagnosed as lipoma arborescens arising in the extra-articular bursa of the knee joint. On MRI, the appearance of lipoma arborescens arising in the extra-articular bursa of the knee joint differed from that of conventional intra-articular lipoma arborescens. In this report, we describe a case of extra-articular lipoma arborescens of the knee joint bursa and discuss the diagnosis and etiology.

  20. Lipoma arborescens arising in the extra-articular bursa of the knee joint.

    Science.gov (United States)

    Minami, Shinji; Miyake, Yusuke; Kinoshita, Hirofumi

    2016-01-01

    Lipoma arborescens arising in the extra-articular bursa of the knee joint is extremely rare. We describe an 11-year-old boy who complained of a gradual swelling mass of the lateral knee joint. Magnetic resonance imaging (MRI) showed a high signal intensity tumor on T1- and T2-weighted images with a thickened septa and nodular lesion that showed low signal intensity. The radiologist suggested the possible differential diagnosis of well-differentiated liposarcoma. At operation, the tumor was found under the iliotibial tract and was not in contact with the knee joint. Histopathologically, this lesion was diagnosed as lipoma arborescens arising in the extra-articular bursa of the knee joint. On MRI, the appearance of lipoma arborescens arising in the extra-articular bursa of the knee joint differed from that of conventional intra-articular lipoma arborescens. In this report, we describe a case of extra-articular lipoma arborescens of the knee joint bursa and discuss the diagnosis and etiology. PMID:27382924

  1. [Basophilic line of the articular cartilage in normal and various pathological states].

    Science.gov (United States)

    Gongadze, L R

    1987-04-01

    Epiphyses of long tubular bones in the man and animals of various age, as well as experimental material of the adjuvant arthritis, with special reference to the basal part of the articular cartilage have been studied by means of histological, histochemical and histometrical methods. The structural-chemical organization of the basophilic line (tidemark) of the articular cartilage ensures its barrier role and participation in regulating selective permeability. Reconstruction of the tidemark in the process of physiological ageing and in cases of the articular pathology is aimed to preserve its integrity and in this way a complete differentiation of the noncalcified and calcified structures is secured. Disturbance of the basophilic line results in changes of the articular selective permeability, in invasion of vessels and structural elements of the bone marrow, and in development of profound distrophic and destructive changes of the cartilage--in deforming artrosis. Deflations in the structural-chemical organization of the tidemark indicate certain disturbances in the state of the system articular cartilage--subchondral bone. These data can be of prognostic importance.

  2. X-ray dark field imaging of human articular cartilage: Possible clinical application to orthopedic surgery

    International Nuclear Information System (INIS)

    Despite its convenience and non-invasiveness on daily clinical use, standard X-ray radiography cannot show articular cartilage. We developed a novel type of X-ray dark field imaging (DFI), which forms images only by a refracted beam with very low background illumination. We examined a disarticulated distal femur and a shoulder joint with surrounding soft tissue and skin, both excised from a human cadaver at the BL20B2 synchrotron beamline at SPring-8. The field was 90 mm wide and 90 mm high. Articular cartilage of the disarticulated distal femur was obvious on DFI, but not on standard X-ray images. Furthermore, DFI allowed visualization in situ of articular cartilage of the shoulder while covered with soft tissue and skin. The gross appearance of the articular cartilage on the dissected section of the proximal humerus was identical to the cartilage shown on the DFI image. These results suggested that DFI could provide a clinically accurate method of assessing articular cartilage. Hence, DFI would be a useful imaging tool for diagnosing joint disease such as osteoarthritis

  3. Pregnane X receptor knockout mice display aging-dependent wearing of articular cartilage.

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    Kotaro Azuma

    Full Text Available Steroid and xenobiotic receptor (SXR and its murine ortholog, pregnane X receptor (PXR, are nuclear receptors that are expressed at high levels in the liver and the intestine where they function as xenobiotic sensors that induce expression of genes involved in detoxification and drug excretion. Recent evidence showed that SXR and PXR are also expressed in bone tissue where they mediate bone metabolism. Here we report that systemic deletion of PXR results in aging-dependent wearing of articular cartilage of knee joints. Histomorphometrical analysis showed remarkable reduction of width and an enlarged gap between femoral and tibial articular cartilage in PXR knockout mice. We hypothesized that genes induced by SXR in chondrocytes have a protective effect on articular cartilage and identified Fam20a (family with sequence similarity 20a as an SXR-dependent gene induced by the known SXR ligands, rifampicin and vitamin K2. Lastly, we demonstrated the biological significance of Fam20a expression in chondrocytes by evaluating osteoarthritis-related gene expression of primary articular chondrocytes. Consistent with epidemiological findings, our results indicate that SXR/PXR protects against aging-dependent wearing of articular cartilage and that ligands for SXR/PXR have potential role in preventing osteoarthritis caused by aging.

  4. Variante faringo-cérvico-braquial del síndrome de Guillain-Barré

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    Iago Pinal-Fernández

    2014-12-01

    Full Text Available El síndrome de Guillain-Barré es una polineuropatía desmielinizante inflamatoria aguda, existiendo variantes locorregionales poco frecuentes como la faringo-cérvico-braquial. Presentamos el caso de un varón de 63 años de edad que acudió a urgencias por debilidad de extremidades superiores y a nivel cervical, con incapacidad para deglutir y masticar, y una insuficiencia respiratoria aguda rápidamente progresiva por debilidad de músculos respiratorios, todo ello secundario a la variante faringo-cérvico-braquial del síndrome de Guillain-Barré. Esta variante, aunque poco frecuente, presenta un patrón clínico y unos criterios diagnósticos bien definidos, importantes de reconocer para poder así iniciar de forma precoz el tratamiento y mejorar el pronóstico, no siempre favorable, de estos pacientes.

  5. Análise da sintomatologia em pacientes com disfunções intra-articulares da articulação temporomandibular Analysis of symptomatology in patients with intra-articular disorders of the temporomandibular joint

    Directory of Open Access Journals (Sweden)

    Sílvio Henrique de Paula DONEGÁ

    1997-01-01

    Full Text Available Foi realizado estudo analisando a sintomatologia em pacientes com disfunções intra-articulares da articulação temporomandibular. A queixa mais citada foi de dor na região pré-auricular (40,7%. Sintomatologia dolorosa articular (63,2% e ruídos articulares (83,3% foram os achados mais comuns ao exame clínico. Os ruídos articulares mais freqüentes foram os estalos (66,6%. Dor muscular ocorreu, em especial, nos músculos pterigóideo medial e lateral e na inserção do temporal. Houve decréscimo na amplitude para a protrusão dentre os movimentos mandibulares máximosThe study analyzed the symptomatology in patients with intra-articular disorders of the temporomandibular joint. The most frequent complaint was pain in the preauricular region (40.7%. Articular pain (63.2% and articular sounds (83.3% were the most common findings during clinical examination. Muscular pain occurred particularly in the medial and lateral pterygoid muscles and at the insertion of the temporalis muscle. The most frequent articular sound was clicking (66.6%. There was a decrease in extent of protrusion among the mandibular border positions.

  6. Effects of altered afferent articular input on sensation, proprioception, muscle tone and sympathetic reflex responses.

    Science.gov (United States)

    Slosberg, M

    1988-10-01

    The influence of afferent articular and periarticular input on muscle tone, joint mobility, proprioception and pain is of considerable interest to practitioners using manipulation. It has long been hypothesized that dysfunctional articulations may generate altered patterns of afferent input. This article reviews the relevant studies that have investigated the impact of articular input on efferent activity under normal conditions and under conditions of altered joint function. The findings suggest that sensory input does have a substantial effect on efferent function and sensation. Furthermore, the studies indicate that the pattern of articular input may be significantly modified by joint inflammation, trauma and effusion and result in changes of muscle tone, joint mobility, proprioception and pain. PMID:3069947

  7. Couple Control Model Implementation on Antagonistic Mono- and Bi-Articular Actuators

    CERN Document Server

    Prattico, Flavio; Yamamoto, Shin-ichiroh

    2014-01-01

    Recently, robot assisted therapy devices are increasingly used for spinal cord injury (SCI) rehabilitation in assisting handicapped patients to regain their impaired movements. Assistive robotic systems may not be able to cure or fully compensate impairments, but it should be able to assist certain impaired functions and ease movements. In this study, a couple control model for lower-limb orthosis of a body weight support gait training system is proposed. The developed leg orthosis implements the use of pneumatic artificial muscle as an actuation system. The pneumatic muscle was arranged antagonistically to form two pair of mono-articular muscles (i.e., hip and knee joints), and a pair of bi-articular actuators (i.e., rectus femoris and hamstring). The results of the proposed couple control model showed that, it was able to simultaneously control the antagonistic mono- and bi-articular actuators and sufficiently performed walking motion of the leg orthosis.

  8. Quantitative characterization of articular cartilage using Mueller matrix imaging and multiphoton microscopy

    Science.gov (United States)

    Ellingsen, Pa˚L. Gunnar; Lilledahl, Magnus Borstad; Aas, Lars Martin Sandvik; Davies, Catharina De Lange; Kildemo, Morten

    2011-11-01

    The collagen meshwork in articular cartilage of chicken knee is characterized using Mueller matrix imaging and multiphoton microscopy. Direction and degree of dispersion of the collagen fibers in the superficial layer are found using a Fourier transform image-analysis technique of the second-harmonic generated image. Mueller matrix images are used to acquire structural data from the intermediate layer of articular cartilage where the collagen fibers are too small to be resolved by optical microscopy, providing a powerful multimodal measurement technique. Furthermore, we show that Mueller matrix imaging provides more information about the tissue compared to standard polarization microscopy. The combination of these techniques can find use in improved diagnosis of diseases in articular cartilage, improved histopathology, and additional information for accurate biomechanical modeling of cartilage.

  9. Extra-Articular Lateral Tenodesis for Anterior Cruciate Ligament Deficient Knee: A Case Report

    Directory of Open Access Journals (Sweden)

    Diego García-Germán

    2013-01-01

    Full Text Available We present the case of an extra-articular lateral tenodesis for an anterior cruciate ligament (ACL deficient knee. A 46-year-old male patient sustained an ACL graft rupture after a motorcycle accident. He complained of rotational instability and giving-way episodes. His previous graft was fixed by an intra-articular femoral staple that was not possible to remove at the time of the ACL revision. A modified Lemaire procedure was then performed. He gained rotational stability and was able to resume his sporting activities. We believe that isolated extra-articular reconstructions may still have a role in selected indications including moderate-demand patients complaining of rotational instability after ACL graft failure.

  10. Treatment of adhesive capsulitis with intra-articular hyaluronate: A systematic review

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    Joshua D Harris

    2011-01-01

    Full Text Available Sodium hyaluronate injection into the glenohumeral joint is a treatment option in the management of adhesive capsulitis of the shoulder. We hypothesized that a systematic review would demonstrate that intra-articular sodium hyaluronate injections would result in significant improvements in passive range-of-motion, shoulder and general clinical outcome measures, and pain scales at short- and mid-term follow-up. Multiple medical databases were searched for levels I-IV evidence with a priori defined specific inclusion and exclusion study criteria. Clinical outcome measures used included Constant score, VAS pain scores, Cho functional scores, JOA scores, and range-of-motion measurements. Seven studies were included (four Level I and three Level IV; 292 subjects, 297 shoulders. Mean subject age was 59.1 years and mean pre-treatment duration of symptoms was 7.3 months. 140 subjects underwent one or multiple hyaluronate injections (120 glenohumeral joint; 20 subacromial bursa. Clinical follow-up was mean 9.0 weeks. Sodium hyaluronate injection into the glenohumeral joint has significantly improved shoulder range-of-motion, constant scores, and pain at short-term follow-up following treatment of adhesive capsulitis. Isolated intra-articular hyaluronate injection has significantly better constant scores than control. Isolated intra-articular hyaluronate injection has equivalent clinical outcomes and range-of-motion compared to intra-articular corticosteroid injection. Intra-articular hyaluronate injection was safe, with no reported complications within the studies in this review. Sodium hyaluronate injection into the glenohumeral joint is a safe, effective treatment in the management of adhesive capsulitis of the shoulder. Short-term evidence indicates that clinical outcomes are better than control and equivalent to intra-articular corticosteroid injection.

  11. MORPHOMETRIC AND MORPHOLOGICAL STUDY OF ARTICULAR FACETS OF THE THORACOLUMBAR VERTEBRAL COLUMN IN NORTH INDIAN POPULATION

    Directory of Open Access Journals (Sweden)

    Rimpi Gupta

    2015-09-01

    Full Text Available Background: The articular processes of thoracolumbar vertebral column play an important role in weight transmission and determining the range and direction of movements between any two vertebrae. Size of these facets has been correlated with the magnitude of stress imposed on them. Purpose of study: The present study has been conducted on the articular processes of 510 vertebrae (thoracic: 360; lumbar:150 with the aim to provide high quality data sets for constructing the models of spine to study mechanics of spinal instrumentation. The length , width and the distance between the right & left superior & inferior articular processes have been measured with the vernier callipers. The presence/ absence of mamillary tubercle has been observed in the present study. Results: The length of thoracic SAFs was almost same at all levels whereas that of the lumbar SAFs increased gradually from L1-L5. However the width showed a variable trend. In case of thoracic IAFs both these parameters showed a variable trend. Whereas in lumbar region, these increased gradually from L1-L5. The distance between two inferior articular processes was more than that between two superior articular processes at almost all levels except T1-T3 & L1-L4 where reverse was true. The mamillary tubercle/process was altogether absent from T-1 to T-8. From T-9 to T-11, the number of vertebral column showing mamillary tubercle increased from 4-19. However at T-12, it was seen in 29 Vertebral columns. In lumbar region, it was well developed in all vertebrae and termed as mamillary process. Conclusion: The measurements obtained by present study reveals the importance of articular facets in understanding basic spinal mechanics and its application with respect to weight transmission.

  12. Comparison of nonlinear mechanical properties of bovine articular cartilage and meniscus.

    Science.gov (United States)

    Danso, E K; Honkanen, J T J; Saarakkala, S; Korhonen, R K

    2014-01-01

    Nonlinear, linear and failure properties of articular cartilage and meniscus in opposing contact surfaces are poorly known in tension. Relationships between the tensile properties of articular cartilage and meniscus in contact with each other within knee joints are also not known. In the present study, rectangular samples were prepared from the superficial lateral femoral condyle cartilage and lateral meniscus of bovine knee joints. Tensile tests were carried out with a loading rate of 5mm/min until the tissue rupture. Nonlinear properties of the toe region, linear properties in larger strains, and failure properties of both tissues were analysed. The strain-dependent tensile modulus of the toe region, Young's modulus of the linear region, ultimate tensile stress and toughness were on average 98.2, 8.3, 4.0 and 1.9 times greater (p<0.05) for meniscus than for articular cartilage. In contrast, the toe region strain, yield strain and failure strain were on average 9.4, 3.1 and 2.3 times greater (p<0.05) for cartilage than for meniscus. There was a significant negative correlation between the strain-dependent tensile moduli of meniscus and articular cartilage samples within the same joints (r=-0.690, p=0.014). In conclusion, the meniscus possesses higher nonlinear and linear elastic stiffness and energy absorption capability before rupture than contacting articular cartilage, while cartilage has longer nonlinear region and can withstand greater strains before failure. These findings point out different load carrying demands that both articular cartilage and meniscus have to fulfil during normal physiological loading activities of knee joints. PMID:24182695

  13. The intra-articular distance within the TMJ during free and loaded closing movements.

    Science.gov (United States)

    Huddleston Slater, J J; Visscher, C M; Lobbezoo, F; Naeije, M

    1999-12-01

    Previous studies on free opening and closing movements of the mandible have demonstrated that the opening movement traces of the condylar kinematic center (i.e., the condylar point for which the protrusive and the opening movement traces coincide) lie closer to the articular eminence than the closing traces. This indicates the presence of an intra-articular distance within the joint during free closing. Since the mandible behaves like a class III biomechanical lever, a counteracting mechanical load on the mandible during closing will press the condyle-disc complex against the articular eminence. Therefore, in this study the hypothesis was tested that the difference between opening and closing movement traces of the kinematic center is reduced when the closing movements are counteracted by a mechanical load. From 10 healthy participants, 20-second movement recordings were obtained by a six-degrees-of-freedom opto-electronic jaw movement recording system (OKAS-3D) for three types of movements: (1) free opening and closing movements, (2) free opening and loaded closing movements (i.e., the participants closed against a small or high manually applied downward-directed force to the chin), and (3) gum chewing. Off-line, the opening and closing movement traces of the kinematic center were reconstructed, and the average difference between the traces (the intra-articular distance) was calculated. The average intraarticular distance was significantly smaller during loaded closing than during free closing, whereas no significant differences were found in the intra-articular distances between the loaded situations of low and high manual loading and contralateral chewing (ANOVA and post hoc Bonferroni multiple comparisons of means test, p<0.005). In conclusion, loading of the mandible during closing movements reduces the intra-articular distance within the temporomandibular joint. PMID:10598911

  14. Mapping the Articular Contact Area of the Long Head of the Biceps Tendon on the Humeral Head

    Directory of Open Access Journals (Sweden)

    Brent J. Morris

    2014-01-01

    Full Text Available The purpose of this investigation was to calculate the contact surface area of the long head of the biceps (LHB in neutral position and abduction. We sought to determine whether the LHB articulates with the humeral head in a consistent pattern comparing articular contact area in neutral position and abduction. Eleven fresh frozen matched cadaveric shoulders were analyzed. The path of the biceps tendon on the articular surface of the humeral head and the total articular surface were digitized using a MicronTracker 2 H3-60 three-dimensional optical tracker. Contact surface area was significantly less in abduction than in neutral position (P=0.002 with a median ratio of 41% (36%, 47.5%. Ratios of contact area in neutral position to full articular surface area were consistent between left and right shoulders (rho=1, P=0.017 as were ratios of abduction area to full articular surface area (rho= 0.97, P=0.005. The articular contact surface area is significantly greater in neutral position than abduction. The ratios of articular contact surface areas to total humeral articular surface areas have a narrow range and are consistent between left and right shoulders of the same cadaver.

  15. Secondary knee instability caused by fracture of the stabilizing insert in a dual-articular total knee

    DEFF Research Database (Denmark)

    Boesen, Morten P; Jensen, Tim Toftgaard; Husted, Henrik

    2004-01-01

    A case of a fractured polyethylene stabilizing insert causing secondary knee instability in a Dual-articular total knee arthroplasty (TKA) is presented. A 65-year-old woman who underwent surgery with a Dual-articular TKA 4 years earlier had a well-functioning prosthesis until a fall, after which...

  16. The influence of surface porosity on gap-healing around intra-articular implants in the presence of migrating particles

    DEFF Research Database (Denmark)

    Rahbek, Ole; Kold, Soren; Zippor, Berit;

    2005-01-01

    -pore coating (Fi) were inserted intra-articular in exact fit or with a 0.75 mm peri-implant gap. A weight-loaded implant device in the distal femur was used. We used a randomized paired design in eight dogs. PE particles were injected repeatedly intra-articular in the knee until the dogs were killed after 8...

  17. Del Piero

    OpenAIRE

    Umbaca, Enzo

    2015-01-01

    Rifacendosi al “Pinturicchio”, epiteto con cui Gianni Agnelli consacrava il calciatore Alessandro Del Piero un “artista del calcio”, Enzo Umbaca scrisse al popolare personaggio invitandolo a collaborare ad una performance durante la quale Pinturicchio-Del Piero avrebbe affrescato il muro di una galleria torinese calciando un pallone macchiato di grafite contro un muro sul quale è appesa la copia di un opera del Pinturicchio.

  18. Interrelationship between mandibular congealer head position in trasncranial view and articular disc position

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Jae Hee; Choi, Soon Chul; You, Dong Soo [Dept. of Oral and Maxillofacial Radiology, Graduate School, Seoul National University, Seoul (Korea, Republic of)

    1995-08-15

    This study was designed to evaluate the interrelationship between the congealer head position in transcranial view and the articular disc position in the arthrography. The condylar positions were assessed by subjective method and linear measurement method on the transcranial view. The subjects for this study consisted of 24 symptomatic joints with normal disc position, 37 joints with anterior disc displacement with reduction and 44 joints with anterior disc displacement without reduction that were classified by arthrotomography under the fluoroscopic guidance. The interrelationship between the condylar head position in transcranial view and the articualr disc position in the arthrography was evaluated by Chi square test. The following results were obtained: 1. There was no significant interrelationship between the position of congealer head in closed mouth state on transcranial view and articular disc position in the arthrography (p>0.05). 2. There was no significant interrelationship between the changes of interarticular distance in 1 inch opening state and articular disc position in the arthrography (p>0.05). 3. There was no significant interrelationship between the position of condylar head related to the apex of articular eminence in 1 inch opening state and articular disc position in the arthrography (p>0.05). 4. There was significant interrelationship between the changes of interarticular distance that is assessed by linear measurement method in maximum opening state and articular disc position in the arthrography (p<0.05), but there was no significant interrelationship when the condylar head position was assessed by subjective method (p>0.05). 5. There was significant interrelationship between the degree of condylar translation in maximum opening state and articular disc position in the arthrography (p<0.01). 6. The correlation coefficient between two methods to assess the position of condylar head were 0.7989: the condylar head position in articular fossa

  19. Interdependência da lateralidade com a estabilidade articular do membro inferior

    OpenAIRE

    Atalaia, Tiago João Viegas

    2015-01-01

    A presença de dominância de um membro inferior em relação ao seu contra lateral, leva a esperar a existência de diferenças funcionais. O objetivo desta tese visou a identificação de diferenças entre membro inferior dominante (DOM) e não dominante (NDOM), no que diz respeito à estabilidade articular (JS), avaliada por medidas de rigidez dinâmica articular (DJS), rigidez vertical (KVERT) ou rigidez de membro inferior (KLEG). A integração de elementos conceptuais e metodológicos foi desenvolvida...

  20. Intra-articular Duration of Durolane™ after Single Injection into the Rabbit Knee

    OpenAIRE

    Edsman, Katarina; Hjelm, Roland; Lärkner, Helena; Nord, Lars I.; Karlsson, Anders; Wiebensjö, Åsa; Höglund, A. Urban; Kenne, Anne Helander; Näsström, Jacques

    2011-01-01

    Objective: The aim of the present study was to investigate the intra-articular duration of Durolane™ in a rabbit model to allow comparison between Durolane™ residence time and data reported for other hyaluronic acid products as well as native hyaluronic acid. Design: 14C-labeled Durolane™ was manufactured by labeling the cross-linker used for stabilization. A single injection of approximately 0.3 mL 14C-labeled Durolane™ was administered intra-articularly in both knee joints of male New Zeala...

  1. Minced articular cartilage--basic science, surgical technique, and clinical application.

    Science.gov (United States)

    McCormick, Frank; Yanke, Adam; Provencher, Matthew T; Cole, Brian J

    2008-12-01

    Minced articular cartilage procedures are attractive surgical approaches for repairing articular cartilage, as they are 1-staged, autologous, and inserted on a carrier that can potentially be placed arthroscopically. The principle of mincing the autologous donor cartilage is to create a larger surface area for cartilage expansion. Placement on a scaffold carrier allows for a chondro-inductive and chondro-conductive milieu. Early animal and preclinical models have demonstrated hyaline-like tissue repair. Further work needs to be conducted in this promising approach. PMID:19011553

  2. A Case of Recurrent Acute Pancreatitis Due to Intra-Articular Corticosteroid Injection

    Directory of Open Access Journals (Sweden)

    Patompong Ungprasert

    2014-03-01

    Full Text Available Context Corticosteroid is a well-established cause of drug-induced pancreatitis. However, acute pancreatitis from intraarticularinjection of corticosteroid has never been described. Case report A 69-year-old male presented with acuteabdominal pain and was diagnosed with acute pancreatitis. The patient had one episode of acute pancreatitis two yearearlier. Both episodes occurred after intra-articular cortisone injection. Investigations for other causes of pancreatitis werenegative. Conclusion We report the first case of acute pancreatitis from intra-articular corticosteroid injection. Physiciansshould be aware of this adverse reaction of corticosteroid that can even occur with local administration.

  3. Rigidez articular - doi: 10.5102/ucs.v3i1.550

    Directory of Open Access Journals (Sweden)

    Gustavo Rocha Velloso

    2008-04-01

    Full Text Available As causas da rigidez articulatória devem ser bem avaliadas quando presentes na história clínica dos pacientes. Fatores intrínsecos e extrínsecos devem ser considerados com cuidado. A qualidade da superfície articulatória, o nivelamento da cartilagem e a fibrose são causas comuns da queixa de rigidez articular. O aumento do líquido sinovial e a hipertrofia sinovial podem ser fatores importantes na restrição do movimento articular.

  4. Analgesic effects of intra-articular botulinum toxin Type B in a murine model of chronic degenerative knee arthritis pain

    Directory of Open Access Journals (Sweden)

    Stephanie Anderson

    2010-09-01

    Full Text Available Stephanie Anderson1,2, Hollis Krug1,2, Christopher Dorman1, Pari McGarraugh1, Sandra Frizelle1, Maren Mahowald1,21Rheumatology Section, Veteran’s Affairs Medical Center, Minneapolis, Minnesota; 2Division of Rheumatology and Autoimmune Diseases, University of Minnesota Medical School, Minneapolis, Minnesota, USAObjective: To evaluate the analgesic effectiveness of intra-articular botulinum toxin Type B (BoNT/B in a murine model of chronic degenerative arthritis pain.Methods and materials: Chronic arthritis was produced in adult C57Bl6 mice by intra-articular injection of Type IV collagenase into the left knee. Following induction of arthritis, the treatment group received intra-articular BoNT/B. Arthritic control groups were treated with intra-articular normal saline or sham injections. Pain behavior testing was performed prior to arthritis, after induction of arthritis, and following treatments. Pain behavior measures included analysis of gait impairment (spontaneous pain behavior and joint tenderness evaluation (evoked pain response. Strength was measured as ability to grasp and cling.Results: Visual gait analysis showed significant impairment of gait in arthritic mice that improved 43% after intra-articular BoNT/B, demonstrating a substantial articular analgesic effect. Joint tenderness, measured with evoked pain response scores, increased with arthritis induction and decreased 49.5% after intra-articular BoNT/B treatment. No improvement in visual gait scores or decrease in evoked pain response scores were found in the control groups receiving intra-articular normal saline or sham injections. Intra-articular BoNT/B was safe, and no systemic effects or limb weakness was noted.Conclusions: This study is the first report of intra-articular BoNT/B for analgesia in a murine model of arthritis pain. The results of this study validate prior work using intra-articular neurotoxins in murine models. Our findings show chronic degenerative arthritis

  5. Efecto antiinflamatorio y antioxidante del extracto hidroalcohólico de Petiveria alliacea

    Directory of Open Access Journals (Sweden)

    César Zaa

    2013-06-01

    Full Text Available Los radicales libres y el daño oxidativo están relacionados con la muerte celular, siendo la peroxidación lipídica un mecanismo que lleva a la destrucción oxidativa de la membrana celular. Además, en procesos inflamatorios, uno de los primeros estadios es la permeabilidad vascular incrementada (formación del edema seguido de la extravasación de los fluídos, migración de leucocitos al sitio dañado y liberación de proteínas pro-inflamatorias. Evaluamos el efecto antioxidante y antiinflamatorio para Petiveria alliacea“mucura”. Para el efecto antioxidante, se evaluó la formación de especies reactivas al ácido tiobarbitúrico como indicador de la peroxidación lipídica. Dosis 200mg/mL de Petiveria alliaceadisminuyó significativamente en un 42% los niveles de MDA comparado con el agua (control negativo. En la evaluación antiinflamatoria, se indujo la inflamación por inyección de car-ragenina (solución al 1%, en la parte subplantar de ratones y en la “bolsa de aire subcutánea” de ratas para la inflamación aguda y crónica respectivamente. En la evaluación antiinflamatoria hay una máxima reducción del edema en un 23,26% a las 4 horas del tratamiento. Para la inflamación crónica hay una reducción del 25,9% y 29,5% del peso y volumen del exudado extraído, respectivamente, así como una reducción del 24% de peso de tejido fibroso. Estos resultados evidencian efecto antioxidante y antiinflamatorio de Petiveria alliacea.

  6. The Safety and Effectiveness of Single and Repeat Dosing of Intra-Articular Anti-Tumour Necrosis Factor Treatment after Failure of Intra-Articular Steroids

    OpenAIRE

    Chia, Justin; POPE, JANET

    2011-01-01

    Objectives: To determine if intra-articular (ia) anti-tumour necrosis factor (TNF) yielded benefit in patients failing ia steroid injections and determine the safety and durability of single and repeated ia anti-TNF treatment in inflammatory arthritis. Methods: Patients with inflammatory arthritis having one or two active joints, and having failed previous ia steroids were injected with ia adalimumab or ia etanercept mixed with triamcinolone and lidocaine via a retrospective chart audit. Resu...

  7. Contribución al conocimiento del árbol de cirián (Crescentia alata Kunth.) variabilidad, selección, morfología, citogenética y fitoquímica.

    OpenAIRE

    Peralta Valverde, Gabriel

    2012-01-01

    El cirián es un árbol medicinal con frutos esféricos que contiene iridoides y polifenoles los que confieren propiedades anti-inflamatorias, antioxidantes y antimicrobianas. La presente investigación se realizó en la región de Tierra Caliente, Michoacán, México en la Depresión del Balsas, la cual es uno de los refugios pleistocénicos más extensos de Norteamérica y centro de origen y domesticación del maíz; el árbol de cirián, es una especie emblemática de esta región y probablemente constituyó...

  8. Interferon beta-1a in chronic inflammatory demyelinating polyneuropathy: case report Interferon beta en polineuropatía crónica inflamatoria desmienlinizante: caso clínico

    Directory of Open Access Journals (Sweden)

    Andrés Maria Villa

    2004-09-01

    Full Text Available Chronic inflammatory demyelinating polyneuropathy (CIDP is an acquired immune-mediated neuropathy. It presents with a course of progression which may be slow and steady or step-wise or relapsing. Sensory ataxic polyneuropathy may be the only clinical manifestation of this disease. Treatment with interferon beta1a (INF beta1a has been tried with different results in patients who were refractory to other, more conventional, immunomodulatory therapies. Here we report on a patient who had a relapsing form of pure sensory ataxic CIDP and who failed to respond to intravenous human immunoglobulin. He was put on INF beta1a for 3 years. During this period he suffered no relapses while his condition stabilized.La polineuropatía crónica inflamatoria desmielinizante (PCID es una neuropatía inmuno-mediada, que presenta un curso clínico primariamente progresivo o en forma de recaídas. Las manifestaciones sensoriales pueden ser su unica forma de expresión clínica. El tratamiento con interferon beta 1a (IFN beta1a ha sido ensayado en varias oportunidades, con diferentes respuestas terapéuticas, en pacientes refractarios a las terapias inmunomoduladoras convencionales. Nosotros comunicamos un paciente con una forma ataxica recurrente de PCID, que no respondió al tratamiento con inmunoglobulina endovenosa. Posteriormente fue tratado con IFN beta 1 a por tres años. Durante el período de seguimiento no mostró nuevas recaídas y su cuadro neurológico se estabilizó.

  9. Hyperhomocysteinemia and methylenetetrahydrofolate reductase 677C→T and 1298A→C mutations in patients with inflammatory bowel disease Hiperhomocisteinemia y mutaciones de la metilentetrahidrofolato reductasa 677C→T y 1298A→C en pacientes con enfermedad inflamatoria intestinal

    Directory of Open Access Journals (Sweden)

    C. Fernández-Miranda

    2005-07-01

    Full Text Available Background: hyperhomocysteinemia has been recently described in patients with inflammatory bowel disease (IBD, that could be related to the increased risk for thrombosis that exists in this disease. The aim of this study was the assessment of hyperhomocysteinemia in patients with IBD and its relation among vitamin B12 and folate levels, and methylenetetrahydrofolate reductase (MTHFR 677C→ T and 1298A→C mutations. Patients and methods: fifty two consecutive patients with IBD were studied (29 women and 23 men; age: mean (standard deviation 41.7 [11.9] years and 186 controls with no difference in age and gender. Hyperhomocysteinemia was considered as homocysteine levels higher than mean plus two standard deviations of the control group (≥ 13 μmol/l. Results: patients had an elevated prevalence of hyperhomocysteinemia (17.3 vs. 3.7%; p = 0.002 and lower folate (7.6 [4.1] vs. 8.9 [3.7] ng/ml; p = 0.01 and B12 vitamin levels (499 [287] vs. 603 [231] pg/ml; p = 0.003. Homocysteinemia was higher (14.3 [5.8] vs. 9.1 [3.9] μmol/l; p = 0.006 in 6 patients (11.5% that had suffered thromboembolism. Frequency of MTHFR 677C→T (13.5 vs. 11.3%; p = 0.66 and 1298A→C (7.8 vs. 7.0%; p = 0.76 mutations was not increased in patients. Odds ratio (OR for IBD in hyperhomocysteinemic patient was 5.51, 95% confidence interval (CI, 1.81-16.76; p = 0.002. Hyperhomocysteinemia was negatively associated with feminine gender (OR 0.08, 95% CI 0.01-0.49; p = 0.006 and folate levels (OR 0.04, 95%CI: 0.007-0.20; p Fundamento: recientemente se ha descrito la existencia de hiperhomocisteinemia en la enfermedad inflamatoria intestinal (EII, que podría estar relacionada con el mayor riesgo de trombosis en esta enfermedad. El objetivo del estudio ha sido evaluar la hiperhomocisteinemia en los pacientes con EII y su relación con las concentraciones de vitamina B12 y folato séricos y con las mutaciones de la metilentetrahidrofolato reductasa (MTHFR 677C→T y 1298A

  10. Age-related accumulation of Maillard reaction products in human articular cartilage collagen

    NARCIS (Netherlands)

    Verzijl, N.; Groot, J. de; Oldehinkel, E.; Bank, R.A.; Thorpe, S.R.; Baynes, J.W.; Bayliss, M.T.; Bijlsma, J.W.J.; Lafeber, F.P.J.G.; TeKoppele, J.M.

    2000-01-01

    Non-enzymic modification of tissue proteins by reducing sugars, the so-called Maillard reaction, is a prominent feature of aging. In articular cartilage, relatively high levels of the advanced glycation end product (AGE) pentosidine accumulate with age. Higher pentosidine levels have been associated

  11. Noncontact evaluation of articular cartilage degeneration using a novel ultrasound water jet indentation system.

    Science.gov (United States)

    Lu, M-H; Zheng, Y P; Huang, Q-H; Ling, C; Wang, Q; Bridal, L; Qin, L; Mak, A

    2009-01-01

    We previously reported a noncontact ultrasound water jet indentation system for measuring and mapping tissue mechanical properties. The key idea was to utilize a water jet as an indenter as well as the coupling medium for high-frequency ultrasound. In this paper, the system was employed to assess articular cartilage degeneration, using stiffness ratio as an indicator of the mechanical properties of samples. Both the mechanical and acoustical properties of intact and degenerated bovine patellar articular cartilage (n = 8) were obtained in situ. It was found that the stiffness ratio was reduced by 44 +/- 17% after the articular cartilage was treated by 0.25% trypsin at 37 degrees C for 4 h while no significant difference in thickness was observed between the intact and degenerated samples. A significant decrease of 36 +/- 20% in the peak-to-peak amplitude of ultrasound echoes reflected from the cartilage surface was also found for the cartilage samples treated by trypsin. The results also showed that the stiffness obtained with the new method highly correlated with that measured using a standard mechanical testing protocol. A good reproducibility of the measurements was demonstrated. The present results showed that the ultrasound water jet indentation system may provide a potential tool for the non-destructive evaluation of articular cartilage degeneration by simultaneously obtaining mechanical properties, acoustical properties, and thickness data. PMID:19011965

  12. Increasing lateral tibial slope: is there an association with articular cartilage changes in the knee?

    Energy Technology Data Exchange (ETDEWEB)

    Khan, Nasir; Shepel, Michael; Leswick, David A.; Obaid, Haron [University of Saskatchewan, Department of Medical Imaging, Royal University Hospital, and College of Medicine, Saskatoon, Saskatchewan (Canada)

    2014-04-15

    The geometry of the lateral tibial slope (LTS) plays an important role in the overall biomechanics of the knee. Through this study, we aim to assess the impact of LTS on cartilage degeneration in the knee. A retrospective analysis of 93 knee MRI scans (1.5 T or 3 T) for patients aged 20-45 years with no history of trauma or knee surgery, and absence of internal derangement. The LTS was calculated using the circle method. Chondropathy was graded from 0 (normal) to 3 (severe). Linear regression analysis was used for statistical analysis (p < 0.05). In our cohort of patients, a statistically significant association was seen between increasing LTS and worsening cartilage degenerative changes in the medial patellar articular surface and the lateral tibial articular surface (p < 0.05). There was no statistically significant association between increasing LTS and worsening chondropathy of the lateral patellar, medial trochlea, lateral trochlea, medial femoral, lateral femoral, and medial tibial articular surfaces. Our results show a statistically significant association between increasing LTS and worsening cartilage degenerative changes in the medial patella and the lateral tibial plateau. We speculate that increased LTS may result in increased femoral glide over the lateral tibial plateau with subsequent increased external rotation of the femur predisposing to patellofemoral articular changes. Future arthroscopic studies are needed to further confirm our findings. (orig.)

  13. Is nephrolithiasis an unrecognized extra-articular manifestation in ankylosing spondylitis?

    DEFF Research Database (Denmark)

    Jakobsen, Ane Krag; Jacobsson, Lennart T H; Patschan, Oliver;

    2014-01-01

    BACKGROUND: Ankylosing spondylitis (AS) is associated with several extra-articular manifestations. Nephrolithiasis (NL) has not been recognized as one of those, however, several factors known to increase the risk of NL are at play in AS patients. The objective was to estimate rates and predictors...

  14. Adverse effects of extra-articular corticosteroid injections: A systematic review

    NARCIS (Netherlands)

    A. Brinks (Tineke); B.W. Koes (Bart); A.C. Volkers (Aloysius); J.A.N. Verhaar (Jan); S.M. Bierma-Zeinstra (Sita)

    2010-01-01

    textabstractBackground. To estimate the occurrence and type of adverse effects after application of an extra-articular (soft tissue) corticosteroid injection. Methods. A systematic review of the literature was made based on a PubMed and Embase search covering the period 1956 to January 2010. Case re

  15. Topographical mapping of biochemical properties of articular cartilage in the equine fetlock joint

    NARCIS (Netherlands)

    Brama, P.A.J.; Tekoppele, J.M.; Bank, R.A.; Karssenberg, D.; Barneveld, A.; Weeren, P.R. van

    2000-01-01

    The aim of this study was to evaluate topographical differences in the biochemical composition of the extracellular matrix of articular cartilage of the normal equine fetlock joint. Water content, DNA content, glycosaminoglycan (GAG) content and a number of characteristics of the collagen network (t

  16. Increasing lateral tibial slope: is there an association with articular cartilage changes in the knee?

    International Nuclear Information System (INIS)

    The geometry of the lateral tibial slope (LTS) plays an important role in the overall biomechanics of the knee. Through this study, we aim to assess the impact of LTS on cartilage degeneration in the knee. A retrospective analysis of 93 knee MRI scans (1.5 T or 3 T) for patients aged 20-45 years with no history of trauma or knee surgery, and absence of internal derangement. The LTS was calculated using the circle method. Chondropathy was graded from 0 (normal) to 3 (severe). Linear regression analysis was used for statistical analysis (p < 0.05). In our cohort of patients, a statistically significant association was seen between increasing LTS and worsening cartilage degenerative changes in the medial patellar articular surface and the lateral tibial articular surface (p < 0.05). There was no statistically significant association between increasing LTS and worsening chondropathy of the lateral patellar, medial trochlea, lateral trochlea, medial femoral, lateral femoral, and medial tibial articular surfaces. Our results show a statistically significant association between increasing LTS and worsening cartilage degenerative changes in the medial patella and the lateral tibial plateau. We speculate that increased LTS may result in increased femoral glide over the lateral tibial plateau with subsequent increased external rotation of the femur predisposing to patellofemoral articular changes. Future arthroscopic studies are needed to further confirm our findings. (orig.)

  17. Presence and mechanism of knee articular cartilage degeneration after meniscal reconstruction in dogs

    NARCIS (Netherlands)

    van Tienen, TG; Heijkants, RGJC; de Groot, JH; Pennings, AJ; Poole, AR; Veth, RPH; Buma, P

    2003-01-01

    Objective: Partial meniscectomy is the golden standard for treating a bucket-handle tear in the meniscus of the knee, but it inevitably leads to articular cartilage degeneration. Surgical creation of an access channel between the lesion and the vascularized synovial lining is intended to induce ingr

  18. Porous polymers for repair and replacement of the knee joint meniscus and articular cartilage

    NARCIS (Netherlands)

    Klompmaker, Jan

    1992-01-01

    The studies presented here were initiated to answer a variety of questions concerning firstly the repair and replacement of the knee joint meniscus and, secondly, the repair of full-thickness defects of articular cartilage. AIMS OF THE STUDIES I To assess the effect of implantation of a porous polym

  19. Presence and mechanism of knee articular cartilage degeneration after meniscal reconstruction in dogs.

    NARCIS (Netherlands)

    Tienen, Tony van; Heijkants, R.G.J.C.; Groot, J.H. de; Pennings, A.J.; Poole, A.R.; Veth, R.P.H.; Buma, P.

    2003-01-01

    OBJECTIVE: Partial meniscectomy is the golden standard for treating a bucket-handle tear in the meniscus of the knee, but it inevitably leads to articular cartilage degeneration. Surgical creation of an access channel between the lesion and the vascularized synovial lining is intended to induce ingr

  20. Combined Intra-Articular and Intravenous Tranexamic Acid Reduces Blood Loss in Total Knee Arthroplasty

    DEFF Research Database (Denmark)

    Nielsen, Christian Skovgaard; Jans, Øivind; Ørsnes, Thue;

    2016-01-01

    BACKGROUND: In total knee arthroplasty, both intravenous (IV) and intra-articular (IA) administration of tranexamic acid (TXA) have been shown to reduce blood loss in several randomized controlled trials, although routine use of systemic TXA is considerably more common. However, to our knowledge...

  1. Preliminary investigation of intrinsic UV fluorescence spectroscopic changes associated with proteolytic digestion of bovine articular cartilage

    Science.gov (United States)

    Lewis, William; Padilla-Martinez, Juan-Pablo; Ortega-Martinez, Antonio; Franco, Walfre

    2016-03-01

    Degradation and destruction of articular cartilage is the etiology of osteoarthritis (OA), an entity second only to cardiovascular disease as a cause of disability in the United States. Joint mechanics and cartilage biochemistry are believed to play a role in OA; an optical tool to detect structural and chemical changes in articular cartilage might offer benefit for its early detection and treatment. The objective of the present study was to identify the spectral changes in intrinsic ultraviolet (UV) fluorescence of cartilage that occur after proteolytic digestion of cartilage. Bovine articular cartilage samples were incubated in varying concentrations of collagenase ranging from 10ug/mL up to 5mg/mL for 18 hours at 37°C, a model of OA. Pre- and post-incubation measurements were taken of the UV excitation-emission spectrum of each cartilage sample. Mechanical tests were performed to determine the pre- and post-digestion force/displacement ratio associated with indentation of each sample. Spectral changes in intrinsic cartilage fluorescence and stiffness of the cartilage were associated with proteolytic digestion. In particular, changes in the relative intensity of fluorescence peaks associated with pentosidine crosslinks (330 nm excitation, 390 nm emission) and tryptophan (290 nm excitation, 340 nm emission) were found to correlate with different degrees of cartilage digestion and cartilage stiffness. In principle, it may be possible to use UV fluorescence spectral data for early detection of damage to articular cartilage, and as a surrogate measure for cartilage stiffness.

  2. Effects of electromagnetic fields on proteoglycan metabolism of bovine articular cartilage explants

    NARCIS (Netherlands)

    De Mattei, M; Pasello, M; Pellati, A; Stabellini, G; Massari, L; Gemmati, D; Caruso, A

    2003-01-01

    Electromagnetic field (EMF) exposure has been proposed for the treatment of osteoarthritis. In this study, we investigated the effects of EMF (75 Hz, 2,3 mT) on proteoglycan (PG) metabolism of bovine articular cartilage explants cultured in vitro, both under basal conditions and in the presence of i

  3. Accuracy of ultrasound-guided injections of thoracolumbar articular process joints in horses

    DEFF Research Database (Denmark)

    Fuglbjerg, Vibeke; Nielsen, J.V.; Thomsen, Preben Dybdahl;

    2010-01-01

    in the literature. Objectives: To evaluate factors of affecting the accuracy of intra-articular injections of the APJs in the caudal thoracolumbar region. Method: One-hundred-and-fifty-four injections with blue dye were performed on APJs including the T14-L6 region in 12 horses subjected to euthanasia for reasons...

  4. Intra-articular drug injections in the combination treatment of rheumatic diseases

    Directory of Open Access Journals (Sweden)

    Yu. A. Olyunin

    2015-01-01

    Full Text Available In clinical practice, intra-articular injections of glucocorticoids (GC are widely used to treat chronic joint inflammatory diseases, osteoarthritis (OA, and extra-articular soft tissue involve-ment. GCs given to patients with chronic arthritis may rapidly suppress joint inflammatory changes and ensure significant clinical improvements well before there is a benefit of prescribed disease-modifying antirheumatic drugs. According to the clinical features of disease, GCs may be used for systemic or local treatment in each specific case and a combination of these two treatments may be performed in some patients. In OA patents, the effect of intra-articular GC injections is less sustained than in those with joint inflammatory diseases and it persists for an average of about 3 weeks. Intra-articular hyaluronic acid is likely to be a more promising treatment for OA. It may also relieve pain considerably and improve joint functions. At the same time the highest effect was noted between weeks 5 and 13 after injection, but improvement also persisted after 14–26 weeks or longer in a number of cases.

  5. THE FUNCTIONAL EFFECTIVENESS OF A CELL-ENGINEERED CONSTRUCT FOR THE REGENERATION OF ARTICULAR CARTILAGE

    Directory of Open Access Journals (Sweden)

    V. I. Sevastianov

    2015-01-01

    Full Text Available The aim of this study is an analysis of the functional effectiveness of a biomedical cell product consisting of a biopolymer microheterogeneous collagen-containing hydrogel (BMCH, human adipose-derived mesenchymal stromal cells (hADMSCs, and chondrogenic induction medium in the regeneration of articular cartilage. Materials and methods. The test model of the adjuvant arthritis was used (female Soviet Chinchilla rabbits with the further development into osteoarthrosis (OA combined with the clinical, biochemical, radiological, and histochemical trials. Results. On Day 92 of the OA model it has been found that the intra-articular introduction of a BMCH with hADMSCs into the left knee joint (n = 3 30 days after the OA modeling, as opposed to the right joint (negative control, n = 3, stimulates the regenerative processes of the cartilaginous tissue structure characterized by the formation of chondrocyte «columns», the emergence of isogenic groups in the intracellular matrix and the regeneration of its structure. Upon the intra-articular introduction of a BMCH (n = 3 such effects are markedly less pronounced. Conclusions. A significant regenerative potential of a cell-engineered construct of human articular tissue (CEC ATh has been proven. It is possible to presume that biostimulating properties of CEC ATh are due to the activating effect of a biomedical cell product on the stem cell migration processes from the surrounding tissue into the injured area with their subsequent differentiation. 

  6. Glucosamine:chondroitin or ginger root extract have little effect on articular cartilage in swine

    Science.gov (United States)

    Sows are culled at a high rate from breeding herds due to musclo-skeletal problems and lameness. Research in our laboratory has shown that even first-parity sows have significant amounts of osteochondritic lesions of their articular cartilage. Glusoamine chondroitin and ginger root extract have both...

  7. Articular cartilage thickness measured with US is not as easy as it appears

    DEFF Research Database (Denmark)

    Torp-Pedersen, S; Bartels, E M; Wilhjelm, Jens E.;

    2011-01-01

    Theoretically, the high spatial resolution of US makes it well suited to monitor the decrease in articular cartilage thickness in osteoarthritis. A requirement is, however, that the borders of the cartilage are correctly identified and that the cartilage is measured under orthogonal insonation....... If US measurements are compared to measurements with other techniques, they should be corrected for the higher sound speed in cartilage....

  8. REPAIR OF SHEEP ARTICULAR-CARTILAGE DEFECTS WITH A RABBIT COSTAL PERICHONDRIAL GRAFT

    NARCIS (Netherlands)

    HOMMINGA, GN; BULSTRA, SK; KUIJER, R; VANDERLINDEN, AJ

    1991-01-01

    A xenograft of costal rabbit perichondrium was transplanted to an articular cartilage defect in a sheep knee. After 12 weeks, cartilage was formed with increased calcification of the basal layer and a mean of 74 percent collagen type 11. The synovium did not show any infiltration, indicating the abs

  9. Intra-articular apatite deposition in mixed connective tissue disease: crystallographic and technetium scanning characteristics.

    OpenAIRE

    Hutton, C W; Maddison, P J; Collins, A J; Berriman, J A

    1988-01-01

    An acute arthritis in a patient with mixed connective tissue disease (MCTD) was found to be associated with intra-articular deposition of carbonated hydroxyapatite crystals. A technetium hydroxymethylene diphosphonate bone scan showed intense uptake in the delayed phase scan of the affected joints. Synovial fluid analysis demonstrated uptake of the radiopharmaceutical drug directly onto the crystals.

  10. Evaluation of influence of proteoglycans on hydration of articular cartilage with the use of ultrasound

    Directory of Open Access Journals (Sweden)

    Yi-yi YANG

    2015-04-01

    Full Text Available Objective To monitor the changes in hydration behaviour of articular cartilage induced by degradation of proteoglycans, and to explore the effect of proteoglycans on hydration behaviour of articular cartilage by using high-frequency ultrasound. Methods Twelve porcine patellae with smooth cartilage surface were prepared and equally divided into two groups: normal group without any enzyme treatment, and trypsin group they were treated with 0.25% trypsin for 8h to digest proteoglycan in the cartilage. The hydration behaviour of the cartilage tissue was scanned by high-frequency ultrasound system with a central frequency of 25MHz. Parameters including cartilage hydration strain and cartilage thickness were measured. The histopathological changes in the articular cartilage were observed under a light microscope. Results It took approximately 20min to reach equilibrium during the hydration process in the normal cartilages, while proteoglycan-degraded cartilage took only about 5min to achieve equilibrium. The equilibrium strain of normal cartilage was 3.5%±0.5%. The degradation of proteoglycans induced a significant decrease in equilibrium strain (1.8%±0.2%, P0.05. Conclusion Proteoglycans play an important role in hydration behaviour of articular cartilage. The degradation of proteoglycans could induce degeneration of cartilage structure and decrease in hydration behaviour after dehydration. DOI: 10.11855/j.issn.0577-7402.2015.03.03

  11. Displaced Intra-articular Fractures of the Calcaneus: with an emphasis on minimally invasive surgery

    NARCIS (Netherlands)

    T. Schepers (Tim)

    2009-01-01

    textabstractDisplaced intra-articular calcaneal fractures are complex injuries. Classically these fractures are treated with open reduction and internal fixation (ORIF) or conservatively. When comparing these two treatment modalities, ORIF has a significantly higher rate of wound complications and c

  12. Relative contribution of matrix metalloprotease and cysteine protease activities to cytokine-stimulated articular cartilage degradation

    DEFF Research Database (Denmark)

    Sondergaard, B C; Henriksen, K; Wulf, H;

    2006-01-01

    OBJECTIVE: Both matrix metalloprotease (MMP) activity and cathepsin K (CK) activity have been implicated in cartilage turnover. We investigated the relative contribution of MMP activity and CK activity in cartilage degradation using ex vivo and in vivo models. METHODS: Bovine articular cartilage...

  13. [Indirect articular lymphography using Tc 99m-labeled Dextran in animals].

    Science.gov (United States)

    Albuquerque, M; Pedroso de Lima, J; Cardoso, A; Mendes, F; Pires, J; Canha, N; Branco, R

    1990-01-01

    Looking for a standardization of the articular lymphoscintigraphy, an experimental research was conducted on 14 dogs injected in the ankle, with 2.5 mCi of 99m Tc-labeled dextran (P.M. = 70,000). Good scintigraphic images of the lymphatic system have been obtained. After having collected blood samples during the experimentation as well as aliquots of organs and of tissues, after the necropsy of the animal, it was verified that the 99m Tc-labeled dextran was useful for quantitative studies of the articular lymphatic drainage. The activities attained, in the drainage lymph nodes of injected articulation, are 152 times superior to those obtained in the kidney and one gramma of popliteal ganglion was 8929 times more active than one gramma of blood. The dextran 70,000, widely used in our clinical routine, can be injected to the human being, without any risk, and it may be the tracer which permits studies of the articular lymphatic drainage and the usage of the lymphoscintigraphy, already in expansion in many other Medicine branches, in the articular studies, nowadays.

  14. Fragmento N-terminal del propéptido natriurético tipo B (NT-proBNP), respuesta inflamatoria y estrés oxidativo en la hipertensión esencial

    OpenAIRE

    Roselló Lletí, Esther

    2011-01-01

    El sistema circulatorio humano es una intrincada red de mecanismos destinados a mantener la homeostasis de presión y flujo pese a las diferentes perturbaciones que sufre y por tanto, una elevación de la presión arterial puede provocar un trastorno en las relaciones de los factores que mantienen este equilibrio. La hipertensión esencial, es el resultado de una compleja interacción entre cambios genéticos, neurohormonales, inflamatorios y bioquímicos. Como consecuencia el paciente hipertenso ti...

  15. Low-intensity infrared laser effects on zymosan-induced articular inflammatory response

    Science.gov (United States)

    Januária dos Anjos, Lúcia Mara; da Fonseca, Adenilson d. S.; Gameiro, Jacy; de Paoli, Flávia

    2015-03-01

    Low-level therapy laser is a phototherapy treatment that involves the application of low power light in the red or infrared wavelengths in various diseases such as arthritis. In this work, we investigated whether low-intensity infrared laser therapy could cause death by caspase-6 apoptosis or DNA damage pathways in cartilage cells after zymosaninduced articular inflammatory process. Inflammatory process was induced in C57BL/6 mouse by intra-articular injection of zymosan into rear tibio-tarsal joints. Thirty animals were divided in five groups: (I) control, (II) laser, (III) zymosan-induced, (IV) zymosan-induced + laser and (V). Laser exposure was performed after zymosan administration with low-intensity infrared laser (830 nm), power 10 mW, fluence 3.0 J/cm2 at continuous mode emission, in five doses. Twenty-four hours after last irradiation, the animals were sacrificed and the right joints fixed and demineralized. Morphological analysis was observed by hematoxylin and eosin stain, pro-apoptotic (caspase-6) was analyzed by immunocytochemistry and DNA fragmentation was performed by TUNEL assay in articular cartilage cells. Inflammatory process was observed in connective tissue near to articular cartilage, in IV and V groups, indicating zymosan effect. This process was decreased in both groups after laser treatment and dexamethasone. Although groups III and IV presented higher caspase-6 and DNA fragmentation percentages, statistical differences were not observed when compared to groups I and II. Our results suggest that therapies based on low-intensity infrared lasers could reduce inflammatory process and could not cause death by caspase-6 apoptosis or DNA damage pathways in cartilage cells after zymosan-induced articular inflammatory process.

  16. Regeneration of Articular Cartilage in Lizard Knee from Resident Stem/Progenitor Cells

    Directory of Open Access Journals (Sweden)

    Lorenzo Alibardi

    2015-09-01

    Full Text Available The epiphysis of femur and tibia in the lizard Podarcis muralis can extensively regenerate after injury. The process involves the articular cartilage and metaphyseal (growth plate after damage. The secondary ossification center present between the articular cartilage and the growth plate is replaced by cartilaginous epiphyses after about one month of regeneration at high temperature. The present study analyzes the origin of the chondrogenic cells from putative stem cells located in the growing centers of the epiphyses. The study is carried out using immunocytochemistry for the detection of 5BrdU-labeled long retaining cells and for the localization of telomerase, an enzyme that indicates stemness. The observations show that putative stem cells retaining 5BrdU and positive for telomerase are present in the superficial articular cartilage and metaphyseal growth plate located in the epiphyses. This observation suggests that these areas represent stem cell niches lasting for most of the lifetime of lizards. In healthy long bones of adult lizards, the addition of new chondrocytes from the stem cells population in the articular cartilage and the metaphyseal growth plate likely allows for slow, continuous longitudinal growth. When the knee is injured in the adult lizard, new populations of chondrocytes actively producing chondroitin sulfate proteoglycan are derived from these stem cells to allow for the formation of completely new cartilaginous epiphyses, possibly anticipating the re-formation of secondary centers in later stages. The study suggests that in this lizard species, the regenerative ability of the epiphyses is a pre-adaptation to the regeneration of the articular cartilage.

  17. A STUDY OF FUNCTIONAL OUT COME OF INTRA - ARTICULAR CALCANEAL FRACTURES TREATED SURGICALLY

    Directory of Open Access Journals (Sweden)

    Prashanth

    2015-10-01

    Full Text Available The calcaneus (O scalcis is the largest and most often fractured tarsal bone. The treatment of calcaneal fractures continues to pose a challenge for the trauma surgeons despite advancement in surgical technique and implant devices. The prognosis for an extra - articular fra cture is uniformly good, but that for an intra - articular fracture is varied. The management of every aspect of intra - articular calcaneal fractures is controversial. Although some studies have demonstrated good results after open reduction and internal fixa tion of intra - articular calcaneal fractures, a few other studies say otherwise. The method of internal fixation is also a point of debate, with various proponents advocating fixation with pins, screws or plate fixation with screws. Even there is no consens us regarding the surgical approach, with many having been described, including medial, lateral, combined medial and lateral, extended lateral and sinus tarsi approaches. So it is the need of the hour, to identify treatment techniques, which use lesser hard ware, to provide better functional outcomes in terms of shorter duration of treatment, better stability and early weight bearing and so also the role of non - operative treatment in the calcaneal fracture management. In this study, we have attempted to evalu ate the results of calcaneal fracture management by open reduction and internal fixation. Ours is a prospective study centered in the Gandhi hospital from Nov 2012 to April 2014 in which 30 patients with intra - articular calcaneal fractures were treated by open reduction and internal fixation and the results were evaluated with AOFAS scoring system. In our analysis, we observed that an anatomical reduction to restore Bohler’s and Gissanes angles associated with rigid internal fixation is essential for better fu n ctional results.

  18. The determination of apoptosis rates on articular cartilages of ovariectomized rats with and without alendronate treatment.

    Science.gov (United States)

    Acar, Nuray; Balkarli, Huseyin; Soyuncu, Yetkin; Ozbey, Ozlem; Celik-Ozenci, Ciler; Korkusuz, Petek; Ustunel, Ismail

    2016-06-01

    Osteoporosis (OP) is a major health problem characterized by compromised bone strength. Osteoarthritis (OA) is a joint disease that progresses slowly and is characterized by breakdown of the cartilage matrix. Alendronate (ALN), a nitrogen-containing bisphosphonate (BIS), inhibits bone loss and increases bone mineralization, and has been used clinically for the treatment of OP. It is still controversial whether BIS is effective in inhibiting the progression of OA. Chondrocyte apoptosis has been described in both human and experimentally induced OA models. In our study we aimed to detect whether ALN could protect articular cartilage from degeneration and reduce apoptosis rates in experimentally OA induced rats. For this rats were ovariectomized (ovex), nine weeks after operation rats were injected 30 µg/kg/week ALN subcutaneously for six weeks. After six weeks articular cartilages were obtained. We did Safranin O staining and Mankin and Pritzker scorings to evaluate degeneration and investigated the expressions of p53, cleaved caspase 3, Poly ADP-ribose (PAR), Poly ADP-ribose polymerase 1 (PARP 1), and applied TUNEL technique to determine apoptotis rates. We found a significant decrease in glycosaminoglycan (GAG) amount and increased apoptosis which indicates damage on articular cartilages of ovex rats. GAG amount was higher and apoptosis rate was lower on articular cartilages of ALN treated ovex rats compared to the ovex group. In contrary to studies showing that early ALN treatment has a protective effect, our study shows late ALN treatment has a chondroprotective effect on articular cartilage since we treated rats nine weeks after ovariectomy. PMID:26631351

  19. Prevalence and characteristics of pneumatized articular tubercle: First large series in Iranian people

    Energy Technology Data Exchange (ETDEWEB)

    Shokri, Abbas; Noruzi-Gangachin, Marruf [Dept. of Oral and Maxillofacial Radiology, Dental School, Hamadan University of Medical Sciences, Hamadan (Iran, Islamic Republic of); Baharvand, Maryam; Mortazavi, Hamed [Dept. of Oral and Maxillofacial Medicine, Dental School, Shahid Beheshti University of Medical Sciences, Tehran (Iran, Islamic Republic of)

    2013-12-15

    This study was performed to determine the prevalence and characteristics of pneumatized articular tubercle or eminence among a defined group of Iranian people. Digital panoramic radiographs of 1694 patients in the Department of Oral and Maxillofacial Radiology, Hamadan Dental School, Iran were evaluated retrospectively to detect the above lesion. Finally, 1563 radiographs were selected according to inclusion criteria. Then, a review was done of 10 large case series found using a MEDLINE search of the literature. Chi-squared test was used to analyze the differences in variables such as age, gender, laterality, and locularity in our case series. The average age of our samples was 32.6±7.63 years. Pneumatized articular tubercle was found in 98 cases, representing a prevalence of 6.2% with a mean age of 22.8±7.9 and a range of 8 to 60 years. Sixty-four (65.3%) pneumatized articular tubercles were unilateral, with 30 lesions on the right and 34 on the left side. Bilateral lesions were found in 34 (34.7%) patients. 52 (53.06%) of the pneumatized articular tubercles were of the unilocular type and 46 (46.94%) were multilocular. The results showed no statistically significant differences regarding age (p=0.454), gender (p=0.634), laterality (p=0.252), or locularity (p=0.807) among the samples. Among ten large case series from other countries, the prevalence of pneumatized articular tubercle (6.2%) in Iranian patients was higher than that of all eight of the case series that used the same detection method as the present study of panoramic radiography.

  20. Effects of Chondroitinase ABC-Mediated Proteoglycan Digestion on Decellularization and Recellularization of Articular Cartilage.

    Directory of Open Access Journals (Sweden)

    Catherine A Bautista

    Full Text Available Articular cartilage has a limited capacity to heal itself and thus focal defects often result in the development of osteoarthritis. Current cartilage tissue engineering strategies seek to regenerate injured tissue by creating scaffolds that aim to mimic the unique structure and composition of native articular cartilage. Decellularization is a novel strategy that aims to preserve the bioactive factors and 3D biophysical environment of the native extracellular matrix while removing potentially immunogenic factors. The purpose of this study was to develop a procedure that can enable decellularization and recellularization of intact articular cartilage matrix. Full-thickness porcine articular cartilage plugs were decellularized with a series of freeze-thaw cycles and 0.1% (w/v sodium dodecyl sulfate detergent cycles. Chondroitinase ABC (ChABC was applied before the detergent cycles to digest glycosaminoglycans in order to enhance donor chondrocyte removal and seeded cell migration. Porcine synovium-derived mesenchymal stem cells were seeded onto the decellularized cartilage scaffolds and cultured for up to 28 days. The optimized decellularization protocol removed 94% of native DNA per sample wet weight, while collagen content and alignment were preserved. Glycosaminoglycan depletion prior to the detergent cycles increased removal of nuclear material. Seeded cells infiltrated up to 100 μm into the cartilage deep zone after 28 days in culture. ChABC treatment enhances decellularization of the relatively dense, impermeable articular cartilage by reducing glycosaminoglycan content. ChABC treatment did not appear to affect cell migration during recellularization under static, in vitro culture, highlighting the need for more dynamic seeding methods.

  1. Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage

    Directory of Open Access Journals (Sweden)

    McCulloch Ryan S

    2012-11-01

    Full Text Available Abstract Background Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were: beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A (cyclophilin A, ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein—zeta polypeptide. The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were (most to least stable succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, succinate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species.

  2. A biphasic finite element study on the role of the articular cartilage superficial zone in confined compression.

    Science.gov (United States)

    Guo, Hongqiang; Maher, Suzanne A; Torzilli, Peter A

    2015-01-01

    The aim of this study was to investigate the role of the superficial zone on the mechanical behavior of articular cartilage. Confined compression of articular cartilage was modeled using a biphasic finite element analysis to calculate the one-dimensional deformation of the extracellular matrix (ECM) and movement of the interstitial fluid through the ECM and articular surface. The articular cartilage was modeled as an inhomogeneous, nonlinear hyperelastic biphasic material with depth and strain-dependent material properties. Two loading conditions were simulated, one where the superficial zone was loaded with a porous platen (normal test) and the other where the deep zone was loaded with the porous platen (upside down test). Compressing the intact articular cartilage with 0.2 MPa stress reduced the surface permeability by 88%. Removing the superficial zone increased the rate of change for all mechanical parameters and decreased the fluid support ratio of the tissue, resulting in increased tissue deformation. Apparent permeability linearly increased after superficial removal in the normal test, yet it did not change in the upside down test. Orientation of the specimen affected the time-dependent biomechanical behavior of the articular cartilage, but not equilibrium behavior. The two tests with different specimen orientations resulted in very different apparent permeabilities, suggesting that in an experimental study which quantifies material properties of an inhomogeneous material, the specimen orientation should be stated along with the permeability result. The current study provides new insights into the role of the superficial zone on mechanical behavior of the articular cartilage. PMID:25465194

  3. Symposium: evidence for the use of intra-articular cortisone or hyaluronic acid injection in the hip.

    Science.gov (United States)

    Chandrasekaran, Sivashankar; Lodhia, Parth; Suarez-Ahedo, Carlos; Vemula, S Pavan; Martin, Timothy J; Domb, Benjamin G

    2016-04-01

    The primary purpose of this review article is to discuss the role of diagnostic, corticosteroid, hyaluronic acid (HA) and platelet rich plasma (PRP) in the treatment of osteoarthritis (OA) and femoroacetabular impingement (FIA). These treatments play an important biological role in the non-operative management of these conditions. Two independent reviewers performed an search of PubMed for articles that contained at least one of the following search terms pertaining to intra-articular hip injection-local anaesthetic, diagnostic, ultrasound, fluoroscopic, image guided, corticosteroid, HA, PRP, OA, labral tears and FAI. Seventy-two full text articles were suitable for inclusion. There were 18 articles addressing the efficacy of diagnostic intra-articular hip injections. With respect to efficacy in OA there were 25 articles pertaining to efficacy of corticosteroid, 22 of HA and 4 of PRP. There were three articles addressing the efficacy of biologics in FAI. Diagnostic intra-articular hip injections are sensitive and specific for differentiating between intra-articular, extra-articular and spinal causes of hip symptoms. Ultrasound and fluoroscopy improves the precision of intra-articular positioning of diagnostic injections. Corticosteroids are more effective than HA and PRP in alleviating pain from hip OA. A higher dose of corticosteroids produces a longer benefit but volume of injection has no significant effect. Intra-articular corticosteroids do not increase infection rates of subsequent arthroplasty. There is currently limited evidence to warrant the routine use of therapeutic injections in the management of labral tears and FIA. PMID:27026814

  4. Bloqueos diagnóstico-terapéuticos de carillas articulares cervicales Diagnostic-therapeutic cervical facet joint blocks

    Directory of Open Access Journals (Sweden)

    M.L. Franco

    2009-03-01

    Full Text Available El 66% de los adultos experimenta dolor cervical a lo largo de su vida y el 54% lo ha experimentado durante los pasados 6 meses. Sólo el 5% presentará dolor crónico intenso acompañado de incapacidad funcional. El 80% del dolor cervical se produce por lesiones óseas o musculares como consecuencia de la degeneración discal y facetaria, a lo que se pueden sumar agentes externos, como traumatismo, sobrepeso, sobrecargas laborales, estrés, etc. El diagnóstico del dolor cervical se basa, fundamentalmente, en la exploración física y en la clínica del paciente. Ante la presencia de dolor cervical irradiado a nuca, cintura escapulohumeral y miembro superior derecho sin patrón radicular con exploración positiva en carillas articulares, están indicados los bloqueos diagnóstico-terapéuticos de éstas. En el trabajo se describe la anatomía de la región cervical, cuyo conocimiento es imprescindible tanto para garantizar los buenos resultados, como para minimizar los riesgos, así como las indicaciones y práctica de la técnica.Cervical pain is experienced by 66% of adults during their lifetime and by 54% in the previous 6 months. Only 5% will experience intense chronic pain with functional disability. Eighty percent of cervical pain is produced by skeletal or muscular lesions as a result of disc and facet degeneration. External causative agents include trauma, overweight, occupational demands, stress, etc. Diagnosis of cervical pain is mainly based on physical examination and clinical findings. In cervical pain radiating to the neck, scapulohumeral girdle and upper right arm without a radicular pattern and positive examination of the facet joints, diagnostic-therapeutic facet joint blocks are indicated. The present article describes the anatomy of the cervical region. Knowledge of this region is essential to guarantee good results and to minimize risks. The indications and practical features of the technique are also discussed.

  5. Jürgen Habermas, democracia, inclusión del otro y patriotismo constitucional desde la ética del discurso

    Directory of Open Access Journals (Sweden)

    Jorge Araya Anabalón

    2011-07-01

    Full Text Available El presente trabajo sobre la ética del discurso de Jurgen Habermas, da cuentade las connotaciones y exigencias para una democracia con legitimidad políticay moral en nuestra sociedad. Entendiendo que una democracia radicalno es algo dado por una sola vez y para siempre, sino el resultado de unproceso comunicativo que permite articular intereses distintos, donde estánen juego la identidad, la solidaridad, la inclusión del “otro” y el patriotismoconstitucional, entre otras cosas. Es decir, se puede corregir y recuperar elideario normativo de la democracia moderna reduciendo la hegemonía de laracionalidad instrumental.

  6. Actividad fibrinolítica sinovial en caballos adultos y potros con patología articular

    OpenAIRE

    Ribera Townsend, Thaïs

    2013-01-01

    La patología articular en el caballo provoca, a corto o largo plazo, cojera en el caballo y es motivo de importantes pérdidas económicas en el mundo deportivo ecuestre. La fisiopatología articular en el caballo comporta una serie de procesos biopatológicos que, si no se detectan y tratan a tiempo, generan un ciclo catabólico autodestructivo que induce la degradación de los propios tejidos articulares. Estudios de medicina humana hace tiempo que han demostrado mediante la determinación de marc...

  7. Secondary knee instability caused by fracture of the stabilizing insert in a dual-articular total knee

    DEFF Research Database (Denmark)

    Boesen, Morten P; Jensen, Tim Toftgaard; Husted, Henrik

    2004-01-01

    A case of a fractured polyethylene stabilizing insert causing secondary knee instability in a Dual-articular total knee arthroplasty (TKA) is presented. A 65-year-old woman who underwent surgery with a Dual-articular TKA 4 years earlier had a well-functioning prosthesis until a fall, after which...... she began complaining of an intermittent, audible clicking; pain; and instability. At surgery, a fractured polyethylene peg was discovered, and the tibial insert was replaced. At follow-up, she remains asymptomatic. This is the first description of a fractured stabilizing insert in a Dual......-articular knee....

  8. DIAGNÓSTICO Y PROPUESTA PARA UNA GESTIÓN INTEGRAL DEL RIESGO EN EL VALLE DE ABURRA: RED RIESGOS

    OpenAIRE

    ARISTIZÁBAL EDIER; VARGAS RICHARD

    2010-01-01

    A partir del análisis de la situación organizacional de las diferentes instituciones en el Valle de Aburrá frente a la reducción de desastres y emergencias asociadas con los fenómenos de origen natural y antrópico, el Área Metropolitana del Valle de Aburrá, como autoridad ambiental y entidad planificadora, hace una propuesta que pretende articular todas las entidades, organizaciones e instituciones públicas y privadas hacia una gestión integral del riesgo, y la cual denominó: Red para la Gest...

  9. Deslumbramientos del mercado

    Directory of Open Access Journals (Sweden)

    Monique Selim

    2006-07-01

    Full Text Available Con eje en los diferentes aspectos que adopta el actual proceso de mundialización de la lógica del mercado y expansión capitalista, aún en países socialistas, el presente artículo plantea la necesidad de una reflexión antropológica susceptible de articular el surgimiento de nuevas formas de dominación y representación simbólicas. Se discute el aparente efecto de unificación intelectual producido por la hegemonía del mercado respecto de hechos sociales heterogéneos dados en la historia reciente de las sociedades contemporáneas. Así, la renovada atracción que ejercen los nuevos escenarios sociales reconfigurados en virtud de las modalidades específicas de encarnación y operación de los procesos globalizantes, llama al estudio antropológico de las coyunturas. La autora despliega una interpretación del socialismo de mercado, conjunción -siempre en tensión- de un monopolio político estatal y una liberalización del mercado económico. En esta perspectiva se reubica el interés de una antropología del presente en relevar localmente, en este caso en Vietnam del Norte, la conformación de un nuevo mercado de demandas simbólicas, donde la reaparición de las prácticas culturales de comunicación con los antepasados expresa la necesidad de reunificación familiar y reconciliación con los muertos en la "larga guerra".Tendo como eixo os aspectos diferentes que adota o processo atual de mundialização da lógica do mercado e de expansão capitalista, até mesmo nos países socialistas, este artigo propoe a necessidade de uma reflexão antropológica capaz de articular o aparecimento de novas formas de dominação e de representação simbólica. Discute-se o efeito de superfície de unificação intelectual causado pela hegemonia do mercado em fatos sociais diversos em sociedades contemporâneas. Assim, a atração renovada de novos fatos sociais pelos modos específicos de incorporação e operação de processos de globaliza

  10. CLINICAL OUTCOME OF INTRA - ARTICULAR DISTAL RADIUS FRACTURES TREATED WITH PLATE AND SCREWS

    Directory of Open Access Journals (Sweden)

    Girish

    2015-02-01

    Full Text Available BACKGROUND : Incidence of fractures of distal radius are increasing due to more geriatric population and road traffic accidents. The fundamental goal of distal radius fractures treatment is restoration of normal or near normal alignment and articular congruity. Restoration of the anatomy and articular surface may prevent the onset of arthritis and improve function. AIMS: To determine clinical outcome of intra - articular distal radius fractures treated with plate and screws. METHOD: 20 patients of intra - articular distal radius fracture treated with open reduction and internal fixation with plate and screws between November 2012 and October 2014 were evaluated. Clinical and radiological evaluation was done at 3 weeks, 3 months, 6 months and one year after surgery. Fractures were cla ssified according to Melone fracture classification. There were 8 type - 2 fractures, 5 type - 3 fractures,4 type - 4 fractures, 2 type - 5 and 1 type - 1 fracture. There were 19 (95% males and 1 (5% females. Age range was between 24 to 50 years with average of 34 years. Fracture was stabilized with the plate and screws at an average of 5 to 7 days post trauma. RESULTS: All fractures had sound clinical and radiological union with an average radiological union time of 14 weeks (range between 2.5 – 6 months. Average palmar flexion of wrist was 56deg, dorsi - flexion was 59deg, radial deviation was17deg, ulnar deviation was 23deg, pronation was 65deg and supination was 72degrees. Gartland and Werley system was used to evaluate the function, 80% of patients had excellent to good results out of which 20% were excellent and 60% were good results, and 15% had fair results and poor results were found in 5%. Average radial inclination was 21 degrees and palmar tilt was 9.5 degrees . CONCLUSION: Open reduction and internal fixation with plate and screws is an excellent, and effective technique in management of intra - articular distal radius fractures in terms of high union

  11. INTERNAL FIXATION OF INTRA ARTICULAR CALCANEUM FRACTURES USING LOCKING COMPRESSION PLATE

    Directory of Open Access Journals (Sweden)

    Neelakrishnan

    2014-10-01

    Full Text Available INTRODUCTION: Calcaneum is the most frequently fractured tarsal bone. Historically intra articular calcaneum fractures treated non-operatively which led to increased morbidity due to in congruency of articular surface resulting in subtalar arthritis. With advent of CT scan, better implants and improved methods of fixation. Operative treatment has now become a standard method. AIM OF THE STUDY: To study the functional and radiological outcome of intra articular calcaneum fractures treated by open reduction and internal fixation using locking compression plate. MATERIALS AND METHODS: Fifteen patients with intra articular calcaneum fractures treated by open reduction and internal fixation using locking compression plate from May 2012 to September 2014 at Rajah Muthiah Medical College Hospital with follow up of 6months. The fractures were classified on the basis of computer tomography (CT findings as Sanders type I to IV. The aim of our treatment is to achieve anatomical reconstruction of all articular surfaces, restore Bohler’s and Gissane angles, to carry out primary stable fixation and begin early mobilization. The patients were evaluated by Modified Maryland Foot Score, with excellent defined as90 - 100 points, good as 75 – 89 points, fair as 50 – 74 points and poor as <50 points. RESULTS: Fourteen patients were male and only one female. Twelve patients sustained fractures due to fall from height, three patients due to RTA. The mean pre-operative Bohler’s angle was 13 degree and mean post-operative angle was 26.2degree. The mean pre-operative Gissane angle was 147.6 degree and the mean post-operative angle was 121 degree. Excellent modified Maryland Foot score were achieved in 9 patients, good in 3, fair in 1patient.Only 2 had poor outcome. CONCLUSION: Surgical treatment of intra-articular calcaneum fractures using locking compression plate by lateral approach has good functional outcome. CT scan is required for understanding the

  12. Clinical utility of ultrasound guidance for intra-articular knee injections: a review

    Directory of Open Access Journals (Sweden)

    Berkoff DJ

    2012-03-01

    Full Text Available David J Berkoff1, Larry E Miller2,3, Jon E Block31Department of Orthopedics, University of North Carolina School of Medicine, Chapel Hill, NC; 2Miller Scientific Consulting Inc, Arden, NC; 3The Jon Block Group, San Francisco, CA, USAAbstract: Intra-articular corticosteroid and hyaluronic acid injections provide short-term symptom amelioration for arthritic conditions involving structural damage or degenerative changes in the knee. Conventional palpation-guided anatomical injections frequently result in inaccurate needle placement into extra-articular tissue and adjacent structures. The purpose of this review was to determine the effect of ultrasound guidance on the accuracy of needle placement, clinical outcomes, and cost-effectiveness in comparison with anatomical landmark-guided intra-articular large joint injections, with particular emphasis on the knee. A total of 13 relevant studies were identified; five studied the knee, seven studied the shoulder, one used both the knee and shoulder, and none studied the hip. Ultrasound was used in seven studies; the remaining studies utilized air arthrography, fluoroscopy, magnetic resonance arthrography, or magnetic resonance imaging. Across all studies (using all imaging modalities and all joints, needle placement accuracy ranged from 63% to 100% with ultrasound and from 39% to 100% with conventional anatomical guidance. Imaging guidance improved the accuracy of intra-articular injections of the knee (96.7% versus 81.0%, P < 0.001 and shoulder (97.3% versus 65.4%, P < 0.001. In particular, ultrasound guidance of knee injections resulted in better accuracy than anatomical guidance (95.8% versus 77.8%, P < 0.001, yielding an odds ratio of 6.4 (95% confidence interval 2.9–14. Ultrasound guidance notably improves injection accuracy in the target intra-articular joint space of large joints including the knee. The enhanced injection accuracy achieved with ultrasound needle guidance directly improves

  13. Quantitative MRI Evaluation of Articular Cartilage Using T2 Mapping Following Hip Arthroscopy for Femoroacetabular Impingement

    Science.gov (United States)

    Mayer, Stephanie W.; Wagner, Naomi; Fields, Kara G.; Wentzel, Catherine; Burge, Alissa; Potter, Hollis G.; Lyman, Stephen; Kelly, Bryan T.

    2016-01-01

    Objectives: Cam-type femoroacetabular impingement (FAI) causes a shearing and delamination injury to the acetabular articular cartilage due to a mismatch between the size of the femoral head and the acetabulum. This mechanism is thought to lead to early osteoarthritis in this population. Cam decompression has been advocated to eliminate impingement, with the ultimate goal of halting the progression of articular cartilage delamination. Although outcomes following this procedure in the young adult population have been favorable at short and medium term follow up, it is not known whether the articular cartilage itself is protected from further injury by changing the biomechanics of the joint with decompression of the cam morphology. The purpose of this study is to compare the pre- and post-operative integrity of the acetabular articular cartilage using T2 mapping to determine if hip arthroscopy is protective of the articular cartilage at short- to medium term follow up. Methods: Males between 18 and 35 years of age who had pre-operative T2 mapping MRIs, underwent hip arthroscopy for cam or mixed-type FAI with an alpha angle greater than 50°, and had at least 2 year follow-up were identified. Post-operative MRIs were performed and T2 relaxation times in the transition zone and weight bearing articular cartilage in the anterosuperior acetabulum at deep and superficial chondral layers were recorded at nine points on three sagittal sequences on pre and post-operative MRIs. A paired t-test was used to compare T2 relaxation values between pre-operative and post-operative scans. Results: Eleven hips were evaluated. Mean age was 26.3 years (range 21 - 35). Mean follow up time to post-operative T2 mapping MRI was 2.6 years (range 2.4 - 2.7). The change in T2 relaxation time was not significantly different between pre- and post-operative MRI scans for any of the nine regions in the deep zone of the acetabular cartilage (p=0.065 - 0.969) or the superficial zone of the

  14. Intra-articular Enzyme Replacement Therapy with rhIDUA is Safe, Well-Tolerated, and Reduces Articular GAG Storage in the Canine Model of Mucopolysaccharidosis Type I

    Science.gov (United States)

    Wang, Raymond Y; Aminian, Afshin; McEntee, Michael F; Kan, Shih-Hsin; Simonaro, Calogera M; Lamanna, William; Lawrence, Roger; Ellinwood, N. Matthew; Guerra, Catalina; Le, Steven Q; Dickson, Patricia I; Esko, Jeffrey D

    2014-01-01

    Background Treatment with intravenous enzyme replacement therapy and hematopoietic stem cell transplantation for mucopolysaccharidosis (MPS) type I does not address joint disease, resulting in persistent orthopedic complications and impaired quality of life. A proof-of-concept study was conducted to determine the safety, tolerability, and efficacy of intra-articular recombinant human iduronidase (IA-rhIDUA) enzyme replacement therapy in the canine MPS I model. Methods Four MPS I dogs underwent monthly rhIDUA injections (0.58 mg/joint) into the right elbow and knee for six months. Contralateral elbows and knees concurrently received normal saline. No intravenous rhIDUA therapy was administered. Monthly blood counts, chemistries, anti-rhIDUA antibody titers, and synovial fluid cell counts were measured. Lysosomal storage of synoviocytes and chondrocytes, synovial macrophages and plasma cells were scored at baseline and one month following the final injection. Results All injections were well-tolerated without adverse reactions. One animal required prednisone for spinal cord compression. There were no clinically significant abnormalities in blood counts or chemistries. Circulating anti-rhIDUA antibody titers gradually increased in all dogs except the prednisone-treated dog; plasma cells, which were absent in all baseline synovial specimens, were predominantly found in synovium of rhIDUA-treated joints at study-end. Lysosomal storage in synoviocytes and chondrocytes following 6 months of IA-rhIDUA demonstrated significant reduction compared to tissues at baseline, and saline-treated tissues at study-end. Mean joint synovial GAG levels in IA-rhIDUA joints was 8.62±5.86 μg/mg dry weight and 21.6±10.4 μg/mg dry weight in control joints (60% reduction). Cartilage heparan sulfate was also reduced in the IA-rhIDUA joints (113±39.5 ng/g wet weight) compared to saline-treated joints (142±56.4 ng/g wet weight). Synovial macrophage infiltration, which was present in all

  15. Caracterización de la disfunción vascular sanguínea y linfática en la cirrosis hepática: Evaluación de la inhibición del factor de crecimiento placentarioy del óxido nítrico como estrategias terapéuticas

    OpenAIRE

    Ribera Sabaté, Jordi

    2012-01-01

    El hígado cirrótico experimenta a lo largo de todo el desarrollo de la enfermedad una serie de cambios estructurales, debidos a la acumulación de matriz extracelular y a la inflamación crónica, que vuelven al tejido hipóxico. La hipoxia tisular provoca la liberación de agentes proangiogénicos, como VEGF-A, que aumentan la irrigación del tejido para compensar la falta de oxígeno. Además, muchas citoquinas sintetizadas por las células inflamatorias o por las células hepáticas estrelladas activa...

  16. Hallazgo de una leucorrea vaginal de carácter no inflamatoria en hembras bovinas. III. Características clínicas y fertilidad (Finding of a vaginal leucorrhea of non-inflammatory character in bovine females. III.Clinical characteristics and fertility

    Directory of Open Access Journals (Sweden)

    Luis O. Alba Gómez

    2005-07-01

    Full Text Available El objetivo de este trabajo fue el de demostrar que la leucorrea vaginal no inflamatoria encontrada en algunas hembras bovinas, no afectaba su eficiencia reproductiva. Para esto se utilizaron 159 novillas y 474 vacas de los genotipos ¾ x ¼ y 5/8 x 3/8 Holstein x Cebú, incorporadas al programa de inseminación artificial. En el 13,8 y 10,5% de las novillas y vacas respectivamente se encontraron colecciones de seudoexudados muco-purulentos y purulentos en sus vaginas. Ninguna de las hembras utilizadas fue tratada antes ni después de ser inseminada. No se encontraron diferencias estadísticas entre las tasas de concepción de las hembras sin y con leucorrea vaginal (79,8 vs 81,8% y (75,0 vs 76,0% para las novillas y las vacas respectivamente. Se comprobó que este tipo de leucorrea vaginal bovina no inflamatoria no afecta la eficiencia reproductiva de esas hembras.

  17. In-vitro intra-articular pressure distribution in the ankle: can it be used for model validation?

    OpenAIRE

    Natsakis, Tassos; Burg, Fien; Dereymaeker, Greta; Jonkers, Ilse; Vander Sloten, Jos

    2014-01-01

    Background Several modelling attempts to predict intra-articular pressure distribution in the ankle joint have been made. Input kinematics for such models can be measured with a variety of techiniques, for validating with intra-articular pressure though, we are limited to in-vitro experimentation. The aim of this study was to investigate the influence of the insertion of a pressure sensor on joint kinematics during in-vitro gait simulations. Methods 10 freshly frozen human cadaveric ...

  18. Alternative technique of cervical spinal stabilization employing lateral mass plate and screw and intra-articular spacer fixation

    OpenAIRE

    Atul Goel

    2013-01-01

    Aim: The author discusses an alternative technique of segmental cervical spinal fixation. Material and Methods: The subtleties of the technique are discussed on the basis of experience with 3 cases with a follow-up of between 30 and 36 months. Technique: The technique involves debridement of facetal articular cartilage, distraction of facets, jamming of ′Goel spacer′ into the articular cavity and fortification of the fixation by lateral mass plate and screw fixation. The ′double-insurance′ me...

  19. Modified arthroscopic double row repair of partial thickness tear of the rotator Cuff involving articular and bursal side

    Directory of Open Access Journals (Sweden)

    Kwon Oh

    2008-01-01

    Full Text Available Partial thickness of rotator cuff tears is considered as a common cause of shoulder disability. Various techniques for arthroscopic repair of partial thickness tear of rotator cuff have been reported in the literature. These techniques have addressed the articular side partial thickness cuff tear. We present an arthroscopic repair of partial thickness tear of rotator cuff involving both articular and bursal surfaces without converting into a full thickness tear. Each side of the tear was repaired with suture anchors separately.

  20. Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage

    OpenAIRE

    Pham, Phuc Van; Bui, Khanh Hong-Thien; Ngo, Dat Quoc; Vu, Ngoc Bich; Truong, Nhung Hai; Phan, Nhan Lu-Chinh; Le, Dung Minh; Duong, Triet Dinh; Nguyen, Thanh Duc; Le, Vien Tuong; Phan, Ngoc Kim

    2013-01-01

    Introduction Adipose-derived stem cells (ADSCs) have been isolated, expanded, and applied in the treatment of many diseases. ADSCs have also been used to treat injured articular cartilage. However, there is controversy regarding the treatment efficiency. We considered that ADSC transplantation with activated platelet-rich plasma (PRP) may improve injured articular cartilage compared with that of ADSC transplantation alone. In this study, we determined the role of PRP in ADSC transplantation t...

  1. Experience of emplying extracorporal shock-wave therapy for treating limb enthesopathies in patients with articular hypermobility syndrome

    OpenAIRE

    VOROTNIKOV ALEXANDER; SANEYEVA GALINA; TSYMBAL ANDREY

    2014-01-01

    Purpose: to study the efficiency of extracorporal shock-wave therapy and the affected area orthopedic relief for treating pain syndrome associated with enthesopathies in patients with articular hypermobility syndrome.75 patients with enthesopathies of various localizations and diagnosed articular hypermobility were observed in 2008-2011, their age varying from 35 to 55 years (median age: 37.5±0.4 years). The treatment mode was out-patient, with a shockwave device PIEZOSON-100 (manufacturer: R...

  2. Effects of low-intensity pulsed ultrasound in repairing injured articular cartilage

    Institute of Scientific and Technical Information of China (English)

    JIA Xiao-lin; CHEN Wen-zhi; ZHOU Kun; WANG Zhi-biao

    2005-01-01

    Objective: To investigate the effects of low-intensity pulsed ultrasound in repairing injured articular cartilage. Methods: Ten adult New Zealand rabbits with bilateral full-thickness osteochondral defects on the cartilage surface of intercondylar fossas were used in this study. The wounds in the left knees were treated with low-intensity pulsed ultrasound as the experimental group. The right knees received no treatment as the control group. All the animals were killed at 8 weeks after injury and the tissues in the wounds were collected for gross appearance grading, histological grading and proteoglycan quantity. Results: The scores of the gross appearance grades, histological grades and the optical density of toluidine blue of the tissues in the experimental group were significantly higher than those of the controls at 8 weeks after injury (P<0.05). Conclusions: Low-intensity pulsed ultrasound can accelerate the repair of injured articular cartilage.

  3. Intra-articular therapy with tumor necrosis factor-α antagonists: an update

    Directory of Open Access Journals (Sweden)

    S. Bello

    2014-03-01

    Full Text Available The aim of the study was to review from the present literature the intra-articular (IA use of the TNF-blocking drugs. A total of 28 papers about this topic were found through a search in PubMed; the first publication’s date was July 2003. These studies include a total of 214 patients affected by 12 different joint diseases that reported a total of 1046 intra-articular therapies carried out in 10 different joint sites. Infliximab and etanercept were the most widely used medications. The safety of this treatment clearly emerges from our analysis, while more difficult was the evaluation of its efficacy. Nevertheless we deduced an ideal patient profile that may better respond to the IA anti-TNF treatment.

  4. Articular Cartilage Thickness Measured with US is Not as Easy as It Appears

    DEFF Research Database (Denmark)

    Torp-Pedersen, Søren; Bartels, E. M.; Wilhjelm, Jens E.;

    2011-01-01

    . Materials and Methods: A literature search limited to the last 10 years of studies applying US to measure cartilage thickness. Results: 15 studies were identified and they referred to another 8 studies describing methods of thickness measurement. 11 of the 15 studies identified the superficial cartilage......Background: Theoretically, the high spatial resolution of US makes it well suited to monitor the decrease in articular cartilage thickness in osteoarthritis. A requirement is, however, that the borders of the cartilage are correctly identified and that the cartilage ismeasured under orthogonal...... insonation. If US measurements are compared to measurements with other techniques, they should be corrected for the higher sound speed in cartilage. Purpose: To study whether investigators correctly identify the articular cartilage, whether they insonate orthogonally, and whether they correct for sound speed...

  5. Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An In Vitro Study

    Directory of Open Access Journals (Sweden)

    Li-ke Luo

    2015-01-01

    Full Text Available As the main active constituent of Andrographis paniculata that was applied in treatment of many diseases including inflammation in ancient China, andrographolide (ANDRO was found to facilitate reduction of edema and analgesia in arthritis. This suggested that ANDRO may be promising anti-inflammatory agent to relieve destruction and degeneration of cartilage after inflammation. In this study, the effect of ANDRO on rabbit articular chondrocytes in vitro was investigated. Results showed that not more than 8 μM ANDRO did no harm to chondrocytes (P0.05. The viability assay, hematoxylin-eosin, safranin O, and immunohistochemical staining also showed better performances in ANDRO groups. As to the doses, 3 μM ANDRO showed the best performance. The results indicate that ANDRO can accelerate proliferation of rabbit articular chondrocytes in vitro and meanwhile maintain the phenotype, which may provide valuable references for further exploration on arthritis.

  6. Large Intra-Articular Anterior Cruciate Ligament Ganglion Cyst, Presenting with Inability to Flex the Knee

    Directory of Open Access Journals (Sweden)

    Jake Sloane

    2010-01-01

    Full Text Available A 41-year-old female presented with a 3-month history of gradually worsening anterior knee pain, swelling and inability to flex the knee. Magnetic resonance imaging (MRI revealed a large intra-articular cystic swelling anterior to the anterior cruciate ligament (ACL, extending into the Hoffa's infrapatellar fat pad. Following manipulation under anaesthesia and arthroscopic debridement of the cyst, the patient's symptoms were relieved with restoration of normal knee motion. ACL ganglion cysts are uncommon intra-articular pathological entities, which are usually asymptomatic and diagnosed incidentally by MRI. This is the first reported case of an ACL cyst being so large as to cause a mechanical block to knee flexion.

  7. LA LÓGICA DEL MERCADO Y EL COMPORTAMIENTO ÉTICO DE LA EMPRESA

    OpenAIRE

    Emmanuel Borgucci

    1999-01-01

    El presente ensayo tiene como finalidad abordar el concepto de la Responsabilidad social dela empresa y como está evolucionando en el transcurso del tiempo hasta desembocar en la obligación existencial de la empresa de articular relaciones que sobrepasan el paradigma de la simplicidad y que se aborde la interacción con la sociedad civil desde una perspectiva multidimensional. Sin embargo, desde la perspectiva de la lógica del mercado no es fácil que la empresa capte las permutaciones que los ...

  8. Innervation and functional characteristics of connective tissues, especially elastic fibers, in human fetal thoracic intervertebral articular capsule and its surroundings.

    Science.gov (United States)

    Shiraishi, Yosuke; Kobayashi, Miya; Yasui, Masaya; Ozaki, Noriyuki; Sugiura, Yasuo

    2003-05-01

    The articular capsules between the thoracic vertebrae, which have physiologically different functions from those of other levels of the vertebrae, have yet to be subjected to neuro-anatomical and fine structural analysis. In the present study, we analyzed serial frozen sections of decalcified thoracic vertebrae in human fetuses, and identified the articular capsule tissue with its unique distribution of elastic fibers. The fine structure of the elastic fibers was studied by transmission electron microscopy. In the early-stage fetus, the fibrous membrane forming the lateral intervertebral articular capsule contained abundant thin elastic fibers consisting of microfibrils. In the late-stage fetus, the lateral capsule of fibrous membrane was occupied by thick elastic fibers. A medial articular capsule, namely the ligamenta flava, contained numerous thick elastic fibers in both early and late-stage fetuses. The distributional differences in nerve fibers between early and late-stage fetuses were determined by immunostaining, using antibodies raised against protein gene product 9.5 (PGP 9.5; ubiquitin carboxyl-terminal hydrolase). Innervation by PGP 9.5 immunoreactive fibers was limited to the areas of the articular capsules near the blood vessels, which may indicate their functional relation with blood flow. No PGP 9.5 immunoreactive fibers were found in the ligamenta flava of the late-stage fetus. Innervation might be directly involved in the development of the intervertebral articular capsules in normal human fetuses.

  9. del Nim

    Directory of Open Access Journals (Sweden)

    Arturo Martínez Rodríguez

    2007-01-01

    Full Text Available Dado su uso específico como fuente de insecticidas naturales de fácil biodegradación, entre otras ventajas, el cultivo del árbol Nim ha sido mundialmente extendido en muchas regiones de Asia, África, Australia y América Latina, incluyendo Cuba. La cosecha del fruto del Nim se hace engorrosa debido a la altura que adquieren estos árboles a los pocos años de sembrados, siendo la cosecha mecánica mediante sacudidores de ramas o del tronco, una posible vía de solución práctica de esta problemática. En el presente trabajo se efectúa, mediante la simulación con el empleo de herramientas CAD-3D y programas de análisis por elementos finitos, un análisis modal abarcador de un número importante de modos de vibración del sistema fruto-pedúnculo del Nim. Como resultado del estudio se pudieron recomendar entornos de frecuencias de las vibraciones a aplicar con mejores expectativas durante la cosecha mecanizada de este producto.

  10. Intra-articular membranous interposition detected by MRI in developmental dysplasia of the hip

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, W.; Itoi, Eiji; Sato, Kozo [Akita Univ. (Japan). Dept. of Orthopedic Surgery

    2000-12-01

    Intra-articular membranous interposition was detected by MRI in the hip joint with residual subluxation of a girl aged 5 years 10 months. This structure, which had low signal intensity on both T1- and T2-weighted images, separated the femoral head from the acetabulum. Histological examination revealed chondrometaplasia, which suggested that this interposition might be transformed to a surface cartilaginous tissue of the secondary acetabulum often observed in residual subluxation of the hip. (orig.)

  11. Derepression of miRNA-138 contributes to loss of the human articular chondrocyte phenotype

    OpenAIRE

    Seidl, C; Martinez-Sanchez, A; Murphy, CL

    2016-01-01

    Objective: To investigate the function of microRNA-138 (miR-138) in human articular chondrocytes (HACs). Methods: The expression of miR-138 in intact cartilage and cultured chondrocytes and the effects of miR-138 overexpression on chondrocyte marker genes were investigated. Targets of miR-138 relevant to chondrocytes were identified and verified by overexpression of synthetic miRNA mimics and inhibitors, luciferase assays, chromatin immunoprecipitation, and RNA immunoprecipitation o...

  12. Fluid collections and juxta-articular cystic lesions of the shoulder: spectrum of MRI findings

    Energy Technology Data Exchange (ETDEWEB)

    Mellado, J.M.; Salvado, E.; Camins, A.; Ramos, A.; Sauri, A. [Institut de Diagnostic per la Imatge, Hospital Joan XXIII, Tarragona (Spain); Merino, X. [Institut de Diagnostic per la Imatge, Hospital Vall' Ebron, Barcelona (Spain); Calmet, J. [Orthopaedic Surgery, Hospital Joan XXIII, Carrer Doctor Mallafre Guasch, Tarragona (Spain)

    2002-03-01

    The MR imaging features of fluid collections and juxta-articular cystic lesions of the shoulder are discussed, with special focus on those related to subacromial impingement and rotator cuff tears. Other more unusual fluid collections and cystic lesions are described, including rice-bodies bursitis, idiopathic synovial osteochondromatosis, dialysis-related amyloid arthropathy, hemophilic arthropathy, infectious conditions, non-infectious inflammatory arthritis, and paralabral cysts. (orig.)

  13. Interplay between environmental factors, articular involvement, and HLA-B27 in patients with psoriatic arthritis.

    OpenAIRE

    Scarpa, R.; DEL PUENTE A; di Girolamo, C; Della Valle, G.; E. Lubrano; Oriente, P

    1992-01-01

    Medical records of 138 patients with psoriatic arthritis and 138 with rheumatoid arthritis were reviewed for the occurrence of an environmental factor triggering arthritis. Twelve (9%) of the patients with psoriatic arthritis had had an acute disorder immediately preceding onset of arthritis (an operation in four cases, articular trauma in three, abortion in two, myocardial infarction, thrombophlebitis, and phosphoric ester intoxication in one case each). Peripheral arthritis occurred in all ...

  14. Comminuted C2 Articular Pillar Fracture in a Patient With Multiple Sclerosis and Recurrent Falls.

    Science.gov (United States)

    Sault, Josiah D; Elliott, James M

    2015-12-01

    The patient was a 60-year-old woman, with long-standing balance deficits due to multiple sclerosis, referred to physical therapy by her primary care physician secondary to increasing fall frequency. Following evaluation, the physical therapist escorted the patient to her primary care physician's office, where a computed tomography scan was immediately performed, revealing a comminuted C2 articular pillar fracture. PMID:26620642

  15. Adverse effects of extra-articular corticosteroid injections: a systematic review

    Directory of Open Access Journals (Sweden)

    Brinks Aaltien

    2010-09-01

    Full Text Available Abstract Background To estimate the occurrence and type of adverse effects after application of an extra-articular (soft tissue corticosteroid injection. Methods A systematic review of the literature was made based on a PubMed and Embase search covering the period 1956 to January 2010. Case reports were included, as were prospective and retrospective studies that reported adverse events of corticosteroid injection. All clinical trials which used extra-articular corticosteroid injections were examined. We divided the reported adverse events into major (defined as those needing intervention or not disappearing and minor ones (transient, not requiring intervention. Results The search yielded 87 relevant studies:44 case reports, 37 prospective studies and 6 retrospective studies. The major adverse events included osteomyelitis and protothecosis; one fatal necrotizing fasciitis; cellulitis and ecchymosis; tendon ruptures; atrophy of the plantar fat was described after injecting a neuroma; and local skin effects appeared as atrophy, hypopigmentation or as skin defect. The minor adverse events effects ranged from skin rash to flushing and disturbed menstrual pattern. Increased pain or steroid flare after injection was reported in 19 studies. After extra-articular injection, the incidence of major adverse events ranged from 0-5.8% and that of minor adverse events from 0-81%. It was not feasible to pool the risk for adverse effects due to heterogeneity of study populations and difference in interventions and variance in reporting. Conclusion In this literature review it was difficult to accurately quantify the incidence of adverse effects after extra-articular corticosteroid injection. The reported adverse events were relatively mild, although one fatal reaction was reported.

  16. Intra-articular Corticosteroid Injection for the Treatment of Idiopathic Adhesive Capsulitis of the Shoulder

    OpenAIRE

    Marx, Robert G.; Malizia, Robert W.; Kenter, Keith; Wickiewicz, Thomas L.; Hannafin, Jo A.

    2007-01-01

    Treatment for idiopathic adhesive capsulitis or frozen shoulder of the shoulder is controversial. The hypothesis of the study is that intra-articular corticosteroid injection in the early stages of idiopathic adhesive capsulitis will lead to a razpid resolution of stiffness and symptoms. This is a retrospective cohort study of only patients with stage 1 or stage 2 adhesive capsulitis. The diagnosis was made by history and physical examination and only when other causes of pain and motion loss...

  17. Guidelines for the Design and Conduct of Clinical Studies in Knee Articular Cartilage Repair

    OpenAIRE

    Mithoefer, Kai; Saris, Daniel B.F.; Farr, Jack; Kon, Elizaveta; Zaslav, Kenneth; Cole, Brian J.; Ranstam, Jonas; Yao, Jian; Shive, Matthew; Levine, David; Dalemans, Wilfried; Brittberg, Mats

    2011-01-01

    Objective: To summarize current clinical research practice and develop methodological standards for objective scientific evaluation of knee cartilage repair procedures and products. Design: A comprehensive literature review was performed of high-level original studies providing information relevant for the design of clinical studies on articular cartilage repair in the knee. Analysis of cartilage repair publications and synopses of ongoing trials were used to identify important criteria for t...

  18. Clinical Testing for Extra-Articular Lateral Knee Pain. A Modification and Combination of Traditional Tests

    Science.gov (United States)

    2008-01-01

    Knee pain is one of the most common problems encountered by recreational and competitive athletes. Pain over the lateral aspect of the knee can be the result of intra or extra articular conditions. The purpose of this clinical suggestion is to present the modification of a traditional clinical test to aide in the differential diagnosis of lateral knee pain. This method has not been described elsewhere and anecdotally has been helpful in the evaluation of patients with lateral knee pain. PMID:21509133

  19. Repair of articular cartilage defects in minipigs by microfracture surgery and BMSCs transplantation

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Objective: To investigate the feasibility of minimal invasive repair of cartilage defect by arthroscope-aided microfracture surgery and autologous transplantation of mesenchymal stem cells. Methods: Bone marrow of minipigs was taken out and the bone marrow derived mesenchymal stem cells (BMSCs) were isolated and cultured to passage 3. Then 6 minipigs were randomly divided into 2 groups with 6 knees in each group. After the articular cartilage defect was induced in each knee. the left defect received microfracture surgery and was injected with 2. 5 ml BMSCs cells at a concentration of 3×107 cells/ml into the articular cavity; while right knee got single microfracture or served as blank control group. The animals were killed at 8 or 16 weeks, and the repair tissue was histologically and immunohistochemically examined for the presence of type Ⅱ collagen and glycosaminoglycans (GAGs) at 8 and 16 weeks. Results:Eight weeks after the surgery, the overlying articular surface of the cartilage defect showed normal color and integrated to adjacent cartilage. And 16 weeks after surgery, hyaline cartilage was observed at the repairing tissues and immunostaining indicated the diffuse presence of this type Ⅱ collagen and GAGs throughout the repair cartilage in the treated defects. Single microfracture group had the repairing of fibro-cartilage, while during the treatment, the defects of blank group were covered with fewer fiber tissues, and no blood capillary growth or any immunological rejection was observed. Conclusion:Microfracture technique and BMSCs transplantation to repair cartilage defect is characterized with minimal invasion and easy operation, and it will greatly promote the regeneration repair of articular cartilage defect.

  20. Magnetic resonance imaging of articular cartilage abnormalities of the far posterior femoral condyle of the knee

    Energy Technology Data Exchange (ETDEWEB)

    Ogino, Shuhei; Huang, Thomas; Watanabe, Atsuya; Iranpour-Boroujeni, Tannaz; Yoshioka, Hiroshi (Dept. of Radiology, Brigham and Women' s Hospital, Boston, MA (United States)), e-mail: hiroshi@uci.edu

    2010-01-15

    Background: Incidental articular cartilage lesions of the far posterior femoral condyle (FPFC) are commonly detected. Whether or not these cartilage lesions are symptomatic or clinically significant is unknown. Purpose: To characterize and assess prevalence of articular cartilage abnormalities of the FPFC and associated bone marrow edema (BME) and/or internal derangements through magnetic resonance (MR) images. Material and Methods: 654 knee MR examinations were reviewed retrospectively. Sagittal fast spin-echo proton density-weighted images with and without fat suppression were acquired with a 1.5T scanner, and were evaluated by two readers by consensus. The following factors were assessed: 1) the prevalence of cartilage abnormalities, 2) laterality, 3) the type of cartilage abnormalities, 4) cartilage abnormality grading, 5) associated BME, 6) complications such as meniscal injury and cruciate ligament injury, and 7) knee alignment (femorotibial angle [FTA]). Results: Articular cartilage abnormalities of the FPFC were demonstrated in 157 of the 654 patients (24%). Of these, 40 patients demonstrated medial and lateral FPFC cartilage abnormalities and were thus counted as 80 cases. Focal lateral FPFC abnormalities were demonstrated in 117 of 197 cases (59.4%), while diffuse lateral FPFC abnormalities were demonstrated in 24 of 197 cases (12.2%). Focal medial FPFC abnormalities were demonstrated in 23 of 197 cases (11.6%), while diffuse medial FPFC abnormalities were demonstrated in 33 of 197 cases (16.8%). No statistically significant pattern of associated BME, FTA, or internal derangements including meniscal and cruciate ligament injury was demonstrated. Conclusion: Articular cartilage abnormalities of the FPFC are common and were demonstrated in 24% of patients or 30% of cases. Lateral FPFC abnormalities occur 2.5 times more frequently than medial FPFC abnormalities and were more frequently focal compared with medial cohorts. BME is associated in 36.5% of cases

  1. MANAGEMENT OF INTRA-ARTICULAR CALCANEAL FRACTURES BY CLOSED REDUCTION WITH PERCUTANEOUS STEINMANN PIN

    OpenAIRE

    Padmanabh, MR; Niaz; Ramachandra,

    2014-01-01

    BACKGROUND & OBJECTIVES: Calcaneum is the most common tarsal bone to fracture and is attended by considerable morbidity. Many treatment techniques are described in literature but indications for specific techniques are vague. This study analyzes the outcome of treatment of intra articular calcaneum fractures treated by Essex-Lopresti technique. METHODS: Between January 2011 and December 2012, 20 patients with tongue-type fracture of Calcaneum who got admitted at Srinivas I...

  2. Collagen metabolism of human osteoarthritic articular cartilage as modulated by bovine collagen hydrolysates

    OpenAIRE

    Saskia Schadow; Hans-Christian Siebert; Günter Lochnit; Jens Kordelle; Markus Rickert; Jürgen Steinmeyer

    2013-01-01

    Destruction of articular cartilage is a characteristic feature of osteoarthritis (OA). Collagen hydrolysates are mixtures of collagen peptides and have gained huge public attention as nutriceuticals used for prophylaxis of OA. Here, we evaluated for the first time whether different bovine collagen hydrolysate preparations indeed modulate the metabolism of collagen and proteoglycans from human OA cartilage explants and determined the chemical composition of oligopeptides representing collagen ...

  3. Freeze-thaw treatment effects on the dynamic mechanical properties of articular cartilage

    Directory of Open Access Journals (Sweden)

    Muldrew Ken

    2010-10-01

    Full Text Available Abstract Background As a relatively non-regenerative tissue, articular cartilage has been targeted for cryopreservation as a method of mitigating a lack of donor tissue availability for transplant surgeries. In addition, subzero storage of articular cartilage has long been used in biomedical studies using various storage temperatures. The current investigation studies the potential for freeze-thaw to affect the mechanical properties of articular cartilage through direct comparison of various subzero storage temperatures. Methods Both subzero storage temperature as well as freezing rate were compared using control samples (4°C and samples stored at either -20°C or -80°C as well as samples first snap frozen in liquid nitrogen (-196°C prior to storage at -80°C. All samples were thawed at 37.5°C to testing temperature (22°C. Complex stiffness and hysteresis characterized load resistance and damping properties using a non-destructive, low force magnitude, dynamic indentation protocol spanning a broad loading rate range to identify the dynamic viscoelastic properties of cartilage. Results Stiffness levels remained unchanged with exposure to the various subzero temperatures. Hysteresis increased in samples snap frozen at -196°C and stored at -80°C, though remained unchanged with exposure to the other storage temperatures. Conclusions Mechanical changes shown are likely due to ice lens creation, where frost heave effects may have caused collagen damage. That storage to -20°C and -80°C did not alter the mechanical properties of articular cartilage shows that when combined with a rapid thawing protocol to 37.5°C, the tissue may successfully be stored at subzero temperatures.

  4. A bi-articular model for scapular-humeral rhythm reconstruction through data from wearable sensors

    OpenAIRE

    Lorussi, Federico; Carbonaro, Nicola; Rossi, Danilo De; Tognetti, Alessandro

    2016-01-01

    Background Patient-specific performance assessment of arm movements in daily life activities is fundamental for neurological rehabilitation therapy. In most applications, the shoulder movement is simplified through a socket-ball joint, neglecting the movement of the scapular-thoracic complex. This may lead to significant errors. We propose an innovative bi-articular model of the human shoulder for estimating the position of the hand in relation to the sternum. The model takes into account bot...

  5. Radiolabeled anti granulocyte antibodies in the detection of osteo-articular prosthesis infection

    International Nuclear Information System (INIS)

    Radiolabeled anti-granulocyte antibodies (immunoscintigraphy) are of demonstrated effectiveness in the diagnosis of infection. The osteo-articular prosthesis infection, instead of its low prevalence, needs an accurate and fast diagnosis. The immunoscintigraphy in this group of patients is a debated subject as the results seem to be related to the experience, interpretation criteria and patients clinical features. In general, immunoscintigraphy is an useful technique with an easy, safe and fast preparation. (author)

  6. Significant Incidence of Extra-Articular Tibia Vara Affects Radiological Outcome of Total Knee Arthroplasty

    OpenAIRE

    Saibaba, Balaji; Dhillon, Mandeep S.; Chouhan, Devendra K; Kanojia, Rajendra K.; Prakash, Mahesh; Bachhal, Vikas

    2015-01-01

    Purpose To identify and quantify the presence of extra-articular tibia vara that might influence the mechanical axis alignment after total knee arthroplasty (TKA). Materials and Methods A total of 48 TKAs in 30 osteoarthritic Indian patients were prospectively evaluated. The hip-knee-ankle angle (HKA), joint line convergence angle, and varus angulation at the femur and tibia were measured from the preoperative and postoperative standing hip-to-ankle radiographs. Four different methods were us...

  7. Tissue engineering for articular cartilage repair – the state of the art

    OpenAIRE

    Johnstone, B.; Alini, M.; M Cucchiarini; GR Dodge; Eglin, D.; F Guilak; Madry, H.; Mata, A.; RL Mauck; CE Semino; MJ Stoddart

    2013-01-01

    Articular cartilage exhibits little capacity for intrinsic repair, and thus even minor injuries or lesions may lead to progressive damage and osteoarthritic joint degeneration, resulting in significant pain and disability. While there have been numerous attempts to develop tissue-engineered grafts or patches to repair focal chondral and osteochondral defects, there remain significant challenges in the clinical application of cell-based therapies for cartilage repair. This paper reviews the cu...

  8. Exosomes from IL-1β stimulated synovial fibroblasts induce osteoarthritic changes in articular chondrocytes

    OpenAIRE

    Kato, Tomohiro; Miyaki, Shigeru; Ishitobi, Hiroyuki; Nakamura, Yoshihiro; Nakasa, Tomoyuki; Lotz, Martin K.; Ochi, Mitsuo

    2014-01-01

    Introduction Osteoarthritis (OA) is a whole joint disease, and characterized by progressive degradation of articular cartilage, synovial hyperplasia, bone remodeling and angiogenesis in various joint tissues. Exosomes are a type of microvesicles (MVs) that may play a role in tissue-tissue and cell-cell communication in homeostasis and diseases. We hypothesized that exosomes function in a novel regulatory network that contributes to OA pathogenesis and examined the function of exosomes in comm...

  9. Chondroprotective Effect of Kartogenin on CD44-Mediated Functions in Articular Cartilage and Chondrocytes

    OpenAIRE

    Ono, Yohei; Ishizuka, Shinya; Knudson, Cheryl B.; Knudson, Warren

    2014-01-01

    Objective: A recent report identified the small molecule kartogenin as a chondrogenic and chondroprotective agent. Since changes in hyaluronan metabolism occur during cartilage degeneration in osteoarthritis, we began studies to determine whether there was a connection between extracellular hyaluronan, CD44–hyaluronan interactions and the effects of kartogenin on articular chondrocytes. Methods: Chondrocytes cultured in monolayers, bioengineered neocartilages, or cartilage explants were treat...

  10. Viscoelastic properties of bovine articular cartilage attached to subchondral bone at high frequencies

    Directory of Open Access Journals (Sweden)

    Shepherd Duncan ET

    2009-06-01

    Full Text Available Abstract Background Articular cartilage is a viscoelastic material, but its exact behaviour under the full range of physiological loading frequencies is unknown. The objective of this study was to measure the viscoelastic properties of bovine articular cartilage at loading frequencies of up to 92 Hz. Methods Intact tibial plateau cartilage, attached to subchondral bone, was investigated by dynamic mechanical analysis (DMA. A sinusoidally varying compressive force of between 16 N and 36 N, at frequencies from 1 Hz to 92 Hz, was applied to the cartilage surface by a flat indenter. The storage modulus, loss modulus and phase angle (between the applied force and the deformation induced were determined. Results The storage modulus, E', increased with increasing frequency, but at higher frequencies it tended towards a constant value. Its dependence on frequency, f, could be represented by, E' = Aloge (f + B where A = 2.5 ± 0.6 MPa and B = 50.1 ± 12.5 MPa (mean ± standard error. The values of the loss modulus (4.8 ± 1.0 MPa mean ± standard deviation were much less than the values of storage modulus and showed no dependence on frequency. The phase angle was found to be non-zero for all frequencies tested (4.9 ± 0.6°. Conclusion Articular cartilage is viscoelastic throughout the full range of frequencies investigated. The behaviour has implications for mechanical damage to articular cartilage and the onset of osteoarthritis. Storage modulus increases with frequency, until the plateau region is reached, and has a higher value than loss modulus. Furthermore, loss modulus does not increase with loading frequency. This means that more energy is stored by the tissue than is dissipated and that this effect is greater at higher frequencies. The main mechanism for this excess energy to be dissipated is by the formation of cracks.

  11. Mycobacterium Avium Arthritis with Extra-articular Abscess in a Patient with Mixed Connective Tissue Disease

    OpenAIRE

    Lee, Choong Won; Sung, Han Dong; Choi, Byong Moon; Kim, Chun Wook; Jun, Su Jin; Min, Sang Jo

    2003-01-01

    A case of Mycobacterium avium arthritis in a 39-year-old female patient with mixed connective tissue disease (MCTD) was reported. An extra-articular abscess had formed outside the knee joint and extended down the calf. A culture was taken of the abscess and synovial fluid disclosed Mycobacteriun avium. This was resistant to most anti-tuberculosis agents. A combination of anti-tuberculosis drugs followed a total resection of the abscess. We concluded that M avium septic arthritis could insidio...

  12. Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An In Vitro Study

    OpenAIRE

    Li-ke Luo; Qing-jun Wei; Lei Liu; Li Zheng; Jin-min Zhao

    2015-01-01

    As the main active constituent of Andrographis paniculata that was applied in treatment of many diseases including inflammation in ancient China, andrographolide (ANDRO) was found to facilitate reduction of edema and analgesia in arthritis. This suggested that ANDRO may be promising anti-inflammatory agent to relieve destruction and degeneration of cartilage after inflammation. In this study, the effect of ANDRO on rabbit articular chondrocytes in vitro was investigated. Results showed that n...

  13. Original Functional Rehabilitation Programme Based on Healing Physiology After Reconstruction of Articular Cartilage in Knee Joint

    OpenAIRE

    Guliyan, Volodymyr; Plenzler, Marcin; Straszewski, Dariusz; Paśnik, Marcin; Korbolewska, Olga; Suszczyński, Wojciech; Śmigielski, Robert

    2014-01-01

    Objectives: The evaluation of the quality of articular cartilage remodelling by means of arthroscopy findings and MRI imaging in a patient, who completed the original rehabilitation program. Methods: The rehabilitation program was conducted according to the Carolina Medical Center rehabilitation protocol. The patient was a 46 years old woman with fourth-degree cartilage damage (Outerbridge classification) located on the right medial femoral condyle of the following size: 1.5x2cm and 1x1.5cm. ...

  14. Arthroscopic study of injuries in articular fractures of distal radius extremity

    OpenAIRE

    Araf, Marcelo; Mattar, Rames

    2014-01-01

    OBJECTIVE: To analyze the incidence of wrist ligament and cartilage associated fractures of the distal radius, through arthroscopy, correlating with AO/ASIF classification. METHODS: Thirty patients aged between 20 and 50 years old, with closed fracture from groups B and C according to AO/ASIF classification were selected. All of them were submitted to wrist arthroscopy to address intra-articular injuries and reduction and osteosynthesis of the fracture. RESULTS: A high incidence of intra-arti...

  15. The study on the mechanical characteristics of articular cartilage in simulated microgravity

    Institute of Scientific and Technical Information of China (English)

    Hai-Jun Niu; Qing Wang; Yue-Xiang Wang; Ang Li; Lian-Wen Sun; Yan Yan; Fan Fan; De-Yu Li; Yu-Bo Fan

    2012-01-01

    The microgravity environment of a long-term space flight may induce acute changes in an astronaut's musculo-skeletal systems.This study explores the effects of simulated microgravity on the mechanical characteristics of articular cartilage.Six rats underwent tail suspension for 14 days and six additional rats were kept under normal earth gravity as controls.Swelling strains were measured using high-frequency ultrasound in all cartilage samples subject to osmotic loading.Site-specific swelling strain data were used in a triphasic theoretical model of cartilage swelling to determine the uniaxial modulus of the cartilage solid matrix.No severe surface irregularities were found in the cartilage samples obtained from the control or tail-suspended groups.For the tail-suspended group,the thickness of the cartilage at a specified site,as determined by ultrasound echo,showed a minor decrease.The uniaxial modulus of articular cartilage at the specified site decreased significantly,from (6.31 ± 3.37) MPa to (5.05 ± 2.98) MPa (p < 0.05).The histology-stained image of a cartilage sample also showed a reduced number of chondrocytes and decreased degree of matrix staining.These results demonstrated that the 14 d simulated microgravity induced significant effects on the mechanical characteristics of articular cartilage.This study is the first attempt to explore the effects of simulated microgravity on the mechanical characteristics of articular cartilage using an osmotic loading method and a triphasic model.The conclusions may provide reference information for manned space flights and a better understanding of the effects of microgravity on the skeletal system.

  16. Intra-articular bupivacaine or bupivacaine and morphine after ACL reconstruction

    OpenAIRE

    Danieli, Marcus Vinicius; Cavazzani Neto, Antonio; Herrera, Paulo Adilson

    2012-01-01

    Objective Reconstructive surgery of the ACL is one of the most commonly performed surgeries today and the control of postoperative pain is part of the priorities of the surgeon. Within the arsenal of analgesia we have the intra-articular application of drugs, and the most studied one is bupivacaine with or without morphine. This study compared the application of bupivacaine with or without morphine with a control group after ACL reconstruction with flexor tendon graft. Methods Forty-five pati...

  17. Manifestações articulares em pacientes com doença de Crohn e retocolite ulcerativa Articular manifestations in patients with Crohn's disease and ulcerative colitis

    Directory of Open Access Journals (Sweden)

    Cristina Costa Duarte Lanna

    2006-06-01

    Full Text Available Os sintomas articulares constituem a manifestação extra-intestinal mais comum em pacientes com doença de Crohn e com retocolite ulcerativa. Reconhecida como artrite colítica, e mais recentemente, artrite enteropática, está classificada como uma das doenças do grupo das espondiloartropatias. Reconhecidamente, há dois padrões de acometimento articular: 1 periférico, habitualmente, associado aos períodos de atividade da doença intestinal e sem associação com o antígeno HLA B27, e 2 axial, caracterizado por espondilite e sacroiliíte, com curso clínico e radiográfico independente da doença intestinal e associado ao HLA B27.Joint involvement is the most commom extraintestinal manifestation in patients with Crohn's disease and ulcerative colitis. The colitic arthritis, lately called enteropathic arthritis, is classified as one of the diseases of the spondyloarthropathies group. There are two patterns of joint involvement: 1 peripheral arthritis, usually coincides with exacerbations of the inflammatory bowel disease and is not associated with the HLA B27 antigen; and 2 axial involvement, characterized by spondylitis and sacroiliitis, which the clinical course is independent of the intestinal disease, and has an association with HLA B27.

  18. Nitrous Oxide sedation for intra-articular injection in juvenile idiopathic arthritis

    Directory of Open Access Journals (Sweden)

    Harel Liora

    2008-01-01

    Full Text Available Abstract Background Intra-articular corticosteroid injection in juvenile idiopathic arthritis (JIA is often associated with anxiety and pain. Recent reports advocate the use of nitrous oxide (NO, a volatile gas with analgesic, anxiolytic and sedative properties. Objective To prospectively evaluate the effectiveness and safety of NO analgesia for intra-articular corticosteroid injection in JIA, and to assess patients and staff satisfaction with the treatment. Methods NO was administered to JIA patients scheduled for joint injection. The patient, parent, physician and nurse completed visual-analog scores (VAS (0–10 for pain, and a 5-point satisfaction scale. Change in heart rate (HR during the procedure was recorded in order to examine physiologic response to pain and stress. Patient's behavior and adverse reactions were recorded. Results 54 procedures (72 joints were performed, 41 females, 13 males; 39 Jewish, 13 Arab; mean age was 12.2 ± 4.7 year. The median VAS pain score for patients, parents, physicians and nurses was 3. The HR increased ≥ 15% in 10 patients. They had higher VAS scores as evaluated by the staff. The median satisfaction level of the parents and staff was 3.0 and 5.0 respectively. Adverse reactions were mild. Conclusion NO provides effective and safe sedation for JIA children undergoing intra-articular injections.

  19. Extreme Postinjection Flare in Response to Intra-Articular Triamcinolone Acetonide (Kenalog).

    Science.gov (United States)

    Young, Porter; Homlar, Kelly C

    2016-01-01

    As intra-articular corticosteroid injections (CSIs) are a common treatment for osteoarthritis, physicians must well understand their potential side effects. Postinjection flares are an acute side effect of intra-articular CSIs, with symptoms ranging from mild joint effusion to disabling pain. The present case involved a severe postinjection flare that occurred after the patient, a 56-year-old woman with moderate osteoarthritis in the left knee, received 2 mL of 1% lidocaine and 2 mL (40 mg) of triamcinolone acetonide (Kenalog). Two hours after injection, she experienced swelling and intense pain in the knee and was unable to ambulate. The knee was aspirated with a return of 25 mL of "butterscotch"-colored fluid. This case is novel in that its acuity of onset, severity of symptoms, and synovial fluid analysis mimicked septic arthritis, which was ultimately ruled out with negative cultures and confirmation of triamcinolone acetonide crystals in the synovial aspirate, viewed by polarized light microscopy. Thus, the patient's reaction represents an acute crystal-induced inflammatory response. Although reactions to an intra-articular CSI of this severity are rare, it is important for treating physicians to inform patients of this potential side effect.

  20. Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.

    Science.gov (United States)

    Mäkelä, J T A; Korhonen, R K

    2016-06-14

    Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid. These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression. Yet, it is known that in indentation, especially at high strain velocities, cartilage can express highly nonlinear response. Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation. Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models. In particular, the collagen fibril network was modeled using eight separate models that implemented five different constitutive equations to describe the nonlinearity. These consisted of linear elastic, nonlinear viscoelastic and multiple nonlinear elastic representations. The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data. Relative difference between the model and experiment was ~3%. Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%. Implementation of the measured volume fractions did not improve the ability of the model to capture the measured mechanical data. These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates. PMID:27130474

  1. Highly nonlinear stress-relaxation response of articular cartilage in indentation: Importance of collagen nonlinearity.

    Science.gov (United States)

    Mäkelä, J T A; Korhonen, R K

    2016-06-14

    Modern fibril-reinforced computational models of articular cartilage can include inhomogeneous tissue composition and structure, and nonlinear mechanical behavior of collagen, proteoglycans and fluid. These models can capture well experimental single step creep and stress-relaxation tests or measurements under small strains in unconfined and confined compression. Yet, it is known that in indentation, especially at high strain velocities, cartilage can express highly nonlinear response. Different fibril reinforced poroelastic and poroviscoelastic models were used to assess measured highly nonlinear stress-relaxation response of rabbit articular cartilage in indentation. Experimentally measured depth-dependent volume fractions of different tissue constituents and their mechanical nonlinearities were taken into account in the models. In particular, the collagen fibril network was modeled using eight separate models that implemented five different constitutive equations to describe the nonlinearity. These consisted of linear elastic, nonlinear viscoelastic and multiple nonlinear elastic representations. The model incorporating the most nonlinearly increasing Young׳s modulus of collagen fibrils as a function of strain captured best the experimental data. Relative difference between the model and experiment was ~3%. Surprisingly, the difference in the peak forces between the experiment and the model with viscoelastic collagen fibrils was almost 20%. Implementation of the measured volume fractions did not improve the ability of the model to capture the measured mechanical data. These results suggest that a highly nonlinear formulation for collagen fibrils is needed to replicate multi-step stress-relaxation response of rabbit articular cartilage in indentation with high strain rates.

  2. Conservative treatment of temporomandibular joint osteoarthrosis: intra-articular injection of sodium hyaluronate.

    Science.gov (United States)

    Guarda-Nardini, L; Masiero, S; Marioni, G

    2005-10-01

    Promising short-term results in the treatment of temporomandibular joint osteoarthrosis with intra-articular injections of sodium hyaluronate (SH) have been reported in preliminary studies. The present prospective study compared long-term outcomes of temporomandibular joint SH injections with those of a conventional non-surgical treatment (bite-plane). Data from three groups of 20 patients with degenerative temporomandibular joint disease were considered. Group A underwent one cycle of five injections of 1 mL SH. Group B underwent a bite-plane treatment for at least 6 months. We considered a control group of 20 patients who refused any treatments. The description of the outcomes was based on objective and subjective parameters after a 6-month follow-up. Sodium hyaluronate and bite-plane treatments significantly improved patients conditions in all considered parameters. No significant differences in outcomes were confirmed by the statistical analysis. The tolerability of SH treatment resulted to be significantly higher. The analysis of results of serial controls in the SH treated group disclosed a significant worsening in pain at rest by comparing 1 and 6 months follow-up. Sodium hyaluronate infiltration resulted a valid non-surgical treatment for temporomandibular joint degenerative disease. Five well-tolerated intra-articular SH injections achieved equivalent results to those of a 6 months bite-plane treatment. We did not diagnose any complications of SH intra-articular injections. Longer time follow-up is necessary to determine the stability of SH properties.

  3. GROWTH DIFFERENTIATION FACTOR-5 STIMULATES THE GROWTH AND ANABOLIC METABOLISM OF ARTICULAR CHONDROCYTES

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Objective To observe the effect of growth differentiation factor-5 (GDF-5) on the growth and anabolic metabolism of articular chondrocytes. Methods The articular chondrocytes isolated from rats were treated with various concentrations of rmGDF-5, and the growth of chondrocytes measured by MTT assay, the cellular cartilage matrices formation detected sulfated glycosaminoglycan by Alcian blue staining and type Ⅱcollagen by RT-PCR. Results After 7 days culture, MTT assay showed that GDF-5 enhanced the growth of chondrocytes in a dose-dependent manner, RT-PCR showed that GDF-5 clearly induced the synthesis of type Ⅱ collagen because of the col2a1 mRNA band more and more strong in a dose-dependent. Chondrocytes were cultured with GDF-5 for 14 days, the intensity of Alcian blue staining was greatly enhanced, especially, at a high concentration of blue-stainable material in a concentration-dependent manner and in a does-dependent manner. Conclusion GDF-5 enhanced the growth of mature articular chondrocytes, and stimulated the cellular cartilage matrices formation in mono-layer culture.

  4. Growth differentiation factor-5 stimulates the growth and anabolic metabolism of articular chondrocytes

    Institute of Scientific and Technical Information of China (English)

    Xu Peng; Guo Xiong; Yao Jianfeng; Zhang Yingang; Klaus von der Mark

    2005-01-01

    Objective: To observe the effect of growth differentiation factor-5 (GDF-5) on the growth and anabolic metabolism of articular chondrocytes. Methods: The articular chondrocytes isolated from rats were treated with various concentrations of rmGDF-5, and the growth of chondrocytes measured by MTT assay, the cellular cartilage matrices formation detected sulfated glycosaminoglycan by Alcian blue staining and type Ⅱ collagen by RT-PCR,the collagen phenotypic expression of chondrocytes detected by immunofluorescence. Results: After 7 days culture,MTT assay showed that GDF-5 enhanced the growth of chondrocytes in a dose-dependent manner, RT-PCR showed that GDF-5 clearly induced the synthesis of type Ⅱ collagen because of the col2a1 mRNA band more and more strong in a dose-dependent. Chondrocytes were cultured with GDF-5 for 14 days, the intensity of Alcian blue staining was greatly enhanced, especially, at a high concentration of 1000ng/ml, and GDF-5 enhanced the accumulation of the Alcian blue-stainable material in a concentration-dependent manner and in a does-dependent manner. Chondrocytes were cultured with GDF-5 for 21 days, immunofluorescent staining of type Ⅱ collagen was clear, the type Ⅰ and X collagen were negative. Conclusion: GDF-5 enhanced the growth of mature articular chondrocytes, and stimulated the cellular cartilage matrices formation, but did not change the collagen phenotypic expression of chondrocytes in mono-layer culture.

  5. Analgesic efficacy of intra-articular morphine after arthroscopic knee surgery in sport injury patients

    Directory of Open Access Journals (Sweden)

    Mitra Yari

    2013-07-01

    Full Text Available BACKGROUND: Anterior Cruciate Ligament (ACL tearing is a common injury among football players. The present study aims to determine the best single-dose of intra-articular morphine for pain relief after arthroscopic knee surgery that, in addition to adequate and long-term analgesia, leads to fewer systemic side effects. METHODS: This clinical trial was conducted on 40 ASA-I athletes. After surgery, all participants received an injection of 20cc of 0.5% intra-articular bupivacaine. In addition, the first control group received a saline injection and 5, 10 and 15 mg of morphine were respectively injected into the joints of the second, third and fourth groups by use of Arthroscopic equipment before the Arthroscopic removal. The amount of pain based on VAS at 1, 2, 4, 6 and 24 hours after surgery, duration of analgesia and the consumption of narcotic drugs were recorded. RESULTS: The VAS scores in the fourth, sixth and twenty-fourth hours after surgery showed a significant difference between the study groups. The average time to the first analgesic request from the bupivacaine plus 15 mg morphine group was significantly longer than other groups and total analgesic requests were significantly lower than other groups. No drowsiness complications were observed in any of the groups in the first 24 hours after injection. CONCLUSION: Application of 15 mg intra-articular morphine after Arthroscopic knee surgery increases the analgesia level as well as its duration (IRCT138902172946N3 .

  6. Growth Differentiation Factor-5 Stimulates the Growth and Anabolic Metabolism of Articular Chondrocytes

    Institute of Scientific and Technical Information of China (English)

    Xu Peng; Yao Jianfeng; Guo Xiong; Zhang Yingang; Klaus von der Mark

    2009-01-01

    Objective: To observe the effect of growth differentiation factor-5 (GDF-5) on the growth and anabolic metabolism of articular chondrocytes. Methods: The articular chondrocytes isolated from rats were treated with various concentrations of rmGDF-5, and the growth of chondrocytes measured by MTr assay, the cellular cartilage matrices formation detected sulfated glycosaminoglycan by Alcian blue staining and type 11 collagen by RT-PCR, the collagen phenotypic expression of chondrocytes detected by immunofluorescence. Results: After 7 days culture, MTF assay showed that GDF-5 enhanced the growth of ehondrocytes in a dose-dependent manner, RT-PCR showed that GDF-5 clearly induced the synthesis of type Ⅱ collagen because of the colal mRNA band more and more strong in a dose-dependent. Chondrocytes were cultured with GDF-5 for 14 days, the intensity of Alcian blue staining was gready enhanced, especially, at a high concentration of 1000ng/ml, and GDF-5 enhanced the accumulation of the Alcian blue-stainable material in a concentration-dependent manner and in a does-dependent manner. Chondrocytes were cultured with GDF-5 for 21 days, immunofluorescent staining of type Ⅱ collagen was clear, the type Ⅰ and Ⅹ collagen were negative. Conclusion: GDF-5 enhanced the growth of mature articular chon-drocytes, and stimulated the cellular cartilage matrices formation, but did not change the collagen phenotypic ex-pression of chondrocytes in mono-layer culture.

  7. Poroelastic response of articular cartilage by nanoindentation creep tests at different characteristic lengths.

    Science.gov (United States)

    Taffetani, M; Gottardi, R; Gastaldi, D; Raiteri, R; Vena, P

    2014-07-01

    Nanoindentation is an experimental technique which is attracting increasing interests for the mechanical characterization of articular cartilage. In particular, time dependent mechanical responses due to fluid flow through the porous matrix can be quantitatively investigated by nanoindentation experiments at different penetration depths and/or by using different probe sizes. The aim of this paper is to provide a framework for the quantitative interpretation of the poroelastic response of articular cartilage subjected to creep nanoindentation tests. To this purpose, multiload creep tests using spherical indenters have been carried out on saturated samples of mature bovine articular cartilage achieving two main quantitative results. First, the dependence of indentation modulus in the drained state (at equilibrium) on the tip radius: a value of 500 kPa has been found using the large tip (400 μm radius) and of 1.7 MPa using the smaller one (25 μm). Secon, the permeability at microscopic scale was estimated at values ranging from 4.5×10(-16) m(4)/N s to 0.1×10(-16) m(4)/N s, from low to high equivalent deformation. Consistently with a poroelastic behavior, the size-dependent response of the indenter displacement disappears when characteristic size and permeability are accounted for. For comparison purposes, the same protocol was applied to intrinsically viscoelastic homogeneous samples of polydimethylsiloxane (PDMS): both indentation modulus and time response have been found size-independent. PMID:24814573

  8. Analgesic effects of intra-articular fentanyl, pethidine and dexamethasone after knee arthroscopic surgery

    Directory of Open Access Journals (Sweden)

    H Saryazd

    2006-07-01

    Full Text Available BACKGROUND: Many different methods have been used in an effort to provide adequate analgesia after knee arthroscopic surgery. In this study analgesic effect of intra-articular fentanyl, pethidine and dexamethasone was compared. METHODS: In a double blind randomized study 48 male patients undergoing knee arthroscopic meniscectomy were allocated to groups receiving intra-articular fentanyl 50 µg or pethidine 20 mg or dexamethasone 8 mg at the end of arthroscopy during general aesthesia. Postoperative pain scores using visual analogue scale were measured and also analgesic requirements and the time of ability to walk were recorded. RESULTS: Pain scores at one, two, six and 24 h after intra-articular injection were not significantly different for fentanyl and pethidine but were higher significantly for dexamethasone at all four mentioned times. The mean average time of ability to walk was significantly longer for dexamethasone. The analgesic requirements during the first 24 h after intraarticular injection were significantly greater only for dexamethasone too. CONCLUSION: Better postoperative analgesia, less pain score and shorter time to walk were achieved by fentanyl and pethidine in comparison to dexamethasone but the results were not significantly different between fentanyl group and pethidine. KEYWORDS: Arthroscopy, opioid, pain.

  9. Contact models of repaired articular surfaces: influence of loading conditions and the superficial tangential zone.

    Science.gov (United States)

    Owen, John R; Wayne, Jennifer S

    2011-07-01

    The superficial tangential zone (STZ) plays a significant role in normal articular cartilage's ability to support loads and retain fluids. To date, tissue engineering efforts have not replicated normal STZ function in cartilage repairs. This finite element study examined the STZ's role in normal and repaired articular surfaces under different contact conditions. Contact area and pressure distributions were allowed to change with time, tension-compression nonlinearity modeled collagen behavior in the STZ, and nonlinear geometry was incorporated to accommodate finite deformation. Responses to loading via impermeable and permeable rigid surfaces were compared to loading via normal cartilage, a more physiologic condition, anticipating the two rigid loading surfaces would bracket that of normal. For models loaded by normal cartilage, an STZ placed over the inferior repair region reduced the short-term axial compression of the articular surface by 15%, when compared to a repair without an STZ. Covering the repair with a normal STZ shifted the flow patterns and strain levels back toward that of normal cartilage. Additionally, reductions in von Mises stress (21%) and an increase in fluid pressure (13%) occurred in repair tissue under the STZ. This continues to show that STZ properties of sufficient quality are likely critical for the survival of transplanted constructs in vivo. However, response to loading via normal cartilage did not always fall within ranges predicted by the rigid surfaces. Use of more physiologic contact models is recommended for more accurate investigations into properties critical to the success of repair tissues.

  10. Intra-Articular Corticosteroids in Addition to Exercise for Reducing Pain Sensitivity in Knee Osteoarthritis

    DEFF Research Database (Denmark)

    Soriano-Maldonado, Alberto; Klokker, Louise; Bartholdy, Cecilie;

    2016-01-01

    OBJECTIVE: To assess the effects of one intra-articular corticosteroid injection two weeks prior to an exercise-based intervention program for reducing pain sensitivity in patients with knee osteoarthritis (OA). DESIGN: Randomized, masked, parallel, placebo-controlled trial involving 100 particip......OBJECTIVE: To assess the effects of one intra-articular corticosteroid injection two weeks prior to an exercise-based intervention program for reducing pain sensitivity in patients with knee osteoarthritis (OA). DESIGN: Randomized, masked, parallel, placebo-controlled trial involving 100...... the injections all participants undertook a 12-week supervised exercise program. Main outcomes were changes from baseline in pressure-pain sensitivity (pressure-pain threshold [PPT] and temporal summation [TS]) assessed using cuff pressure algometry on the calf. These were exploratory outcomes from a...... analyzed. The mean group difference in changes from baseline at week 14 was 0.6 kPa (95% CI: -1.7 to 2.8; P = 0.626) for PPT and 384 mm×sec (95% CI: -2980 to 3750; P = 0.821) for TS. CONCLUSIONS: These results suggest that adding intra-articular corticosteroid injection 2 weeks prior to an exercise program...

  11. Is Mandibular Fossa Morphology and Articular Eminence Inclination Associated with Temporomandibular Dysfunction?

    Science.gov (United States)

    Paknahad, Maryam; Shahidi, Shoaleh; Akhlaghian, Marzieh; Abolvardi, Masoud

    2016-01-01

    Statement of the Problem Finding a significant relationship between temporomandibular joint (TMJ) morphology and the incidence of temporomandibular dysfunction (TMD) may help early prediction and prevention of these problems. Purpose The purpose of the present study was to determine the morphology of mandibular fossa and the articular eminence inclination in patients with TMD and in control group using cone beam computed tomography (CBCT). Materials and Method The CBCT data of bilateral TMJs of 40 patients with TMD and 23 symptom-free cases were evaluated. The articular eminence inclination, as well as the glenoid fossa depth and width of the mandibular fossa were measured. The paired t-test was used to compare these values between two groups. Results The articular eminence inclination and glenoid fossa width and depth were significantly higher in patients with TMD than in the control group (p TMD than in the control group. Glenoid fossa width and depth were higher in patients with TMD than that in the control group. This information may shed light on the relationship between TMJ morphology and the incidence of TMD. PMID:27284559

  12. Treatment of intra-articular fractures of the distal radius: fluoroscopic or arthroscopic reduction?

    Science.gov (United States)

    Varitimidis, S E; Basdekis, G K; Dailiana, Z H; Hantes, M E; Bargiotas, K; Malizos, K

    2008-06-01

    In a randomised prospective study, 20 patients with intra-articular fractures of the distal radius underwent arthroscopically- and fluoroscopically-assisted reduction and external fixation plus percutaneous pinning. Another group of 20 patients with the same fracture characteristics underwent fluoroscopically-assisted reduction alone and external fixation plus percutaneous pinning. The patients were evaluated clinically and radiologically at follow-up of 24 months. The Disabilities of the Arm, Shoulder, and Hand (DASH) questionnaire and modified Mayo wrist score were used at 3, 9, 12 and 24 months postoperatively. In the arthroscopically- and fluoroscopically-assisted group, triangular fibrocartilage complex tears were found in 12 patients (60%), complete or incomplete scapholunate ligament tears in nine (45%), and lunotriquetral ligament tears in four (20%). They were treated either arthroscopically or by open operation. Patients who underwent arthroscopically- and fluoroscopically-assisted treatment had significantly better supination, extension and flexion at all time points than those who had fluoroscopically-assisted surgery. The mean DASH scores were similar for both groups at 24 months, whereas the difference in the mean modified Mayo wrist scores remained statistically significant. Although the groups are small, it is clear that the addition of arthroscopy to the fluoroscopically-assisted treatment of intra-articular distal radius fractures improves the outcome. Better treatment of associated intra-articular injuries might also have been a reason for the improved outcome. PMID:18539672

  13. Analysis of friction between articular cartilage and polyvinyl alcohol hydrogel artificial cartilage.

    Science.gov (United States)

    Li, Feng; Wang, Anmin; Wang, Chengtao

    2016-05-01

    Many biomaterials are being used to repair damaged articular cartilage. In particular, poly vinyl alcohol hydrogel has similar mechanical properties to natural cartilage under compressive and shearing loading. Here, three-factor and two-level friction experiments and long-term tests were conducted to better evaluate its tribological properties. The friction coefficient between articular cartilage and the poly vinyl alcohol hydrogel depended primarily on the three factors of load, speed, and lubrication. When the speed increased from 10 to 20 mm/s under a load of 10 N, the friction coefficient increased from 0.12 to 0.147. When the lubricant was changed from Ringer's solution to a hyaluronic acid solution, the friction coefficient decreased to 0.084 with loads as high as 22 N. The poly vinyl alcohol hydrogel was severely damaged and lost its top surface layers, which were transferred to the articular cartilage surface. Wear was observed in the surface morphologies, which indicated the occurrence of surface adhesion of bovine cartilage. Surface fatigue and adhesive wear was the dominant wear mechanism. PMID:26970769

  14. Diffusion and near-equilibrium distribution of MRI and CT contrast agents in articular cartilage

    International Nuclear Information System (INIS)

    Charged contrast agents have been used both in vitro and in vivo for estimation of the fixed charge density (FCD) in articular cartilage. In the present study, the effects of molecular size and charge on the diffusion and equilibrium distribution of several magnetic resonance imaging (MRI) and computed tomography (CT) contrast agents were investigated. Full thickness cartilage disks (Oe = 4.0 mm, n = 64) were prepared from fresh bovine patellae. Contrast agent (gadopentetate: Magnevist (registered) , gadodiamide: Omniscan(TM), ioxaglate: Hexabrix(TM) or sodium iodide: NaI) diffusion was allowed either through the articular surface or through the deep cartilage. CT imaging of the samples was conducted before contrast agent administration and after 1, 5, 9, 16, 25 and 29 h (and with three samples after 2, 3, 4 and 5 days) diffusion using a clinical peripheral quantitative computed tomography (pQCT) instrument. With all contrast agents, the diffusion through the deep cartilage was slower when compared to the diffusion through the articular surface. With ioxaglate, gadopentetate and gadodiamide it took over 29 h for diffusion to reach the near-equilibrium state. The slow diffusion of the contrast agents raise concerns regarding the validity of techniques for FCD estimation, as these contrast agents may not reach the equilibrium state that is assumed. However, since cartilage composition, i.e. deep versus superficial, had a significant effect on diffusion, imaging of the nonequilibrium diffusion process might enable more accurate assessment of cartilage integrity.

  15. Diffusion and near-equilibrium distribution of MRI and CT contrast agents in articular cartilage

    Energy Technology Data Exchange (ETDEWEB)

    Silvast, Tuomo S; Toeyraes, Juha [Department of Clinical Neurophysiology, Kuopio University Hospital, PO Box 1777, 70211 Kuopio (Finland); Kokkonen, Harri T; Jurvelin, Jukka S [Department of Physics, University of Kuopio, PO Box 1627, 70211 Kuopio (Finland); Quinn, Thomas M [Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, Quebec H3A 2B2 (Canada); Nieminen, Miika T [Department of Diagnostic Radiology, Oulu University Hospital, PO Box 50, 90029, Oulu (Finland)], E-mail: Tuomo.Silvast@uku.fi

    2009-11-21

    Charged contrast agents have been used both in vitro and in vivo for estimation of the fixed charge density (FCD) in articular cartilage. In the present study, the effects of molecular size and charge on the diffusion and equilibrium distribution of several magnetic resonance imaging (MRI) and computed tomography (CT) contrast agents were investigated. Full thickness cartilage disks (Oe = 4.0 mm, n = 64) were prepared from fresh bovine patellae. Contrast agent (gadopentetate: Magnevist (registered) , gadodiamide: Omniscan(TM), ioxaglate: Hexabrix(TM) or sodium iodide: NaI) diffusion was allowed either through the articular surface or through the deep cartilage. CT imaging of the samples was conducted before contrast agent administration and after 1, 5, 9, 16, 25 and 29 h (and with three samples after 2, 3, 4 and 5 days) diffusion using a clinical peripheral quantitative computed tomography (pQCT) instrument. With all contrast agents, the diffusion through the deep cartilage was slower when compared to the diffusion through the articular surface. With ioxaglate, gadopentetate and gadodiamide it took over 29 h for diffusion to reach the near-equilibrium state. The slow diffusion of the contrast agents raise concerns regarding the validity of techniques for FCD estimation, as these contrast agents may not reach the equilibrium state that is assumed. However, since cartilage composition, i.e. deep versus superficial, had a significant effect on diffusion, imaging of the nonequilibrium diffusion process might enable more accurate assessment of cartilage integrity.

  16. Diffusion and near-equilibrium distribution of MRI and CT contrast agents in articular cartilage.

    Science.gov (United States)

    Silvast, Tuomo S; Kokkonen, Harri T; Jurvelin, Jukka S; Quinn, Thomas M; Nieminen, Miika T; Töyräs, Juha

    2009-11-21

    Charged contrast agents have been used both in vitro and in vivo for estimation of the fixed charge density (FCD) in articular cartilage. In the present study, the effects of molecular size and charge on the diffusion and equilibrium distribution of several magnetic resonance imaging (MRI) and computed tomography (CT) contrast agents were investigated. Full thickness cartilage disks (Ø = 4.0 mm, n = 64) were prepared from fresh bovine patellae. Contrast agent (gadopentetate: Magnevist((R)), gadodiamide: Omniscan, ioxaglate: Hexabrix or sodium iodide: NaI) diffusion was allowed either through the articular surface or through the deep cartilage. CT imaging of the samples was conducted before contrast agent administration and after 1, 5, 9, 16, 25 and 29 h (and with three samples after 2, 3, 4 and 5 days) diffusion using a clinical peripheral quantitative computed tomography (pQCT) instrument. With all contrast agents, the diffusion through the deep cartilage was slower when compared to the diffusion through the articular surface. With ioxaglate, gadopentetate and gadodiamide it took over 29 h for diffusion to reach the near-equilibrium state. The slow diffusion of the contrast agents raise concerns regarding the validity of techniques for FCD estimation, as these contrast agents may not reach the equilibrium state that is assumed. However, since cartilage composition, i.e. deep versus superficial, had a significant effect on diffusion, imaging of the nonequilibrium diffusion process might enable more accurate assessment of cartilage integrity. PMID:19864699

  17. Potencial del agua del suelo

    OpenAIRE

    Bustamante Heliodoro

    2012-01-01

    La energía potencial del agua presenta diferencias de un punto del suelo a otro; esas diferencias son las que originan el movimiento del agua de acuerdo a la tendencia universal de la materia en el sentido de moverse de donde la energía potencial es mayor a donde dicha energía es menor. En el suelo el agua en consecuencia se mueve hacia donde su energía decrece hasta lograr su estado de equilibrio. Se desprende entonces que la cantidad de energía potencial absoluta contenida en el agua, no es...

  18. Efeitos do resfriamento e aquecimento articular no desempenho funcional do ombro

    Directory of Open Access Journals (Sweden)

    Dayane de Oliveira Estevam

    2015-06-01

    Full Text Available INTRODUÇÃO: Um dos recursos mais utilizados na reabilitação de lesões musculoesqueléticas é a termoterapia por subtração de calor (crioterapia, enquanto a termoterapia por adição de calor é considerada o procedimento mais antigo de reabilitação física. Entretanto, há poucas evidências que tenham investigado os efeitos desses recursos sobre o desempenho de membros superiores. OBJETIVO: Comparar o efeito do resfriamento e do aquecimento articular sobre o desempenho funcional do membro superior. MÉTODOS: Trinta e quatro voluntários (22,23 ± 2,17 anos; 22,39 ± 2,53 kg/m2, de ambos os sexos, foram divididos aleatoriamente em um dos três grupos: 1 grupo crioterapia GCR (n=10: submetidos ao resfriamento articular por compressas frias; 2 grupo termoterapia - GTE (n=10: submetidos ao aquecimento articular por ondas curtas e 3 grupo controle - GCO (n=14, não submetidos a qualquer intervenção. Os voluntários foram avaliados, pré e pós-intervenção, quanto ao desempenho funcional de membros superiores por meio dos testes de estabilidade da extremidade superior em cadeia cinética fechada (TEESCCF e das condições de equilíbrio em apoio bimanual sobre o baropodômetro. Ainda, os voluntários foram avaliados quanto ao desempenho funcional virtual por meio do jogo Mario Kart (Nintendo Wii (r . RESULTADOS: Houve melhora significativa nos valores pós-intervenção no TEESCCF para o GCR (p0,05. Entretanto, na condição de olhos fechados, houve piora de desempenho para GTE (p=0,04 e melhora de desempenho para o GCO (p=0,02. Não houve alteração significativa no desempenho funcional virtual para os três grupos (p>0,05. CONCLUSÃO: Embora não tenha favorecido o desempenho funcional em todos os testes reais e virtuais utilizados, o resfriamento articular foi mais efetivo que o aquecimento articular para manter o desempenho muscular do membro superior, especialmente nas condições de equilíbrio sobre membros superiores na condi

  19. Repairing articular cartilage defects with tissue-engineering cartilage in rabbits

    Institute of Scientific and Technical Information of China (English)

    SONG Hong-xing; LI Fo-bao; SHEN Hui-liang; LIAO Wei-ming; LIU Miao; WANG Min; CAO Jun-ling

    2006-01-01

    Objective: To investigate the effect of cancellous bone matrix gelatin (BMG) engineered with allogeneic chondrocytes in repairing articular cartilage defects in rabbits.Methods: Chondrocytes were seeded onto three-dimensional cancellous BMG and cultured in vitro for 12 days to prepare BMG-chondrocyte complexes. Under anesthesia with 2.5% pentobarbital sodium (1 ml/kg body weight), articular cartilage defects were made on the right knee joints of 38 healthy New Zealand white rabbits (regardless of sex, aged 4-5 months and weighing 2.5-3 kg) and the defects were then treated with 2.5 % trypsin.Then BMG-chondrocyte complex (Group A, n=18 ),BMG ( Group B, n=10), and nothing ( Group C, n=10)were implanted into the cartilage defects, respectively. The repairing effects were assessed by macroscopic, histologic,transmission electron microscopic (TEM) observation,immunohistochemical examination and in situ hybridization detection, respectively, at 2, 4, 8, 12 and 24 weeks after operation.Results: Cancellous BMG was degraded within 8 weeks after operation. In Group A, lymphocyte infiltration was observed around the graft. At 24 weeks after operation, the cartilage defects were repaired by cartilage tissues and the articular cartilage and subchondral bone were soundly healed. Proteoglycan and type Ⅱ collagen were detected in the matrix of the repaired tissues by Safranin-O staining and immunohistochemical staining,respectively. In situ hybridization proved gene expression of type Ⅱ collagen in the cytoplasm of chondrocytes in the repaired tissues. TEM observation showed that chondrocytes and cartilage matrix in repaired tissues were almost same as those in the normal articular cartilage. In Group B, the defects were repaired by cartilage-fibrous tissues. In Group C, the defects were repaired only by fibrous tissues.Conclusions : Cancellous BMG can be regarded as the natural cell scaffolds for cartilage tissue engineering.Articular cartilage defects can be repaired by

  20. Coeficiente de fricción y mecanismos de degradación superficial en materiales usados para la construcción de implantes de juntas articulares

    Directory of Open Access Journals (Sweden)

    Juan Alejandro Uribe A.

    2004-01-01

    Full Text Available El presente estudio evaluó los materiales más usados en nuestro medio para la fabricación de implantes de juntas articulares, evidenciando los mecanismos de deterioro superficial presentes en tres tribosistemas: a pin de acero AISI 316LVM contra un disco de acero AISI 316L, b pin de acero inoxidable AISI 316LVM contra disco de polietileno de ultra alto peso molecular (UHMWPE y c pin de una aleación Ti6Al4V contra disco de polietileno de ultra alto peso molecular (UHMWPE, con velocidad de deslizamiento de 0.58 m/s y diferentes cargas normales. Se adaptó una máquina de ensayos de desgaste normalizada del tipo pin – disco (norma ASTM G 99, de tal forma que permitiera sumergir el sistema en una solución de suero de sangre de bovino con una concentración de proteínas de 0.03 g/ml, la cual simula el ataque corrosivo al que se ven sometidos los materiales en las juntas implantadas dentro del cuerpo humano. Se midieron la variación de la fuerza y el coeficiente de fricción en el tiempo para estudiar el comportamiento del tribosistema, caracterizando las superficies desgastadas macro y microscópicamente con el fin de identificar los fenómenos de deterioro superficial. La adhesión fue el mecanismo predominante en el deslizamiento de los pares metal – polímero, mientras que la combinación de adhesión y abrasión fue responsable por un daño mucho mayor en el sistema metal-metal.

  1. Controversies in the management of intra-articular fractures of distal humerus in adults

    Directory of Open Access Journals (Sweden)

    Sudhir Babhulkar

    2011-01-01

    Full Text Available Background: The surgical approach, type of olecranon osteotomy, method of stabilization of osteotomy, type of fracture stabilization, orthogonal vs parallel plate fixation, need for transposition of ulnar nerve, place for primary total elbow replacement, and type of rehabilitation schedule after surgical fracture treatment are the controversial issues in the treatment of complex intra-articular distal humerus fractures (C2 and C3 in adults. Severe comminution, bone loss, and osteoporosis at the site of distal articular fractures of humerus often lead to unsatisfactory results due to inadequate fixation. We hereby report the outcome of a series of intracondylar fractures of the humerus treated by open reduction and internal fixation and discuss the controversies in light of published literature. Materials and Methods: One hundred and eighty-four patients of intra-articular fractures of distal humerus (C2 and C3 were operated by posterior transolecranon approach between January 1980 and December 2008. Initially, in the first part Chevron intra-articular osteotomy (n=108 was performed out of which 94 have been published in another publication. In later second part (1993 onward, extra-articular olecranon osteotomy (n=76 was routinely performed. Both columns were stably fixed by orthogonal methods; (n=174 however, during the last 2 years, in 10 patients with severe comminution with bone loss, stabilization was achieved by parallel plating. The osteotomy was routinely stabilized by tension band wiring with two parallel K-wires introduced up to the anterior ulnar cortex. The results were evaluated by the staging system of Caja et al. at a minimum follow-up of 2 years. Results: In the first part of the study (n=94, there was delayed union in 4% (n=4, with the fracture taking more than 20 weeks for union. There was delayed union of ulnar osteotomy (n=3 and failure of one tension band wiring, requiring revision. Some loss of motion was seen in 20% of

  2. Additive Effects of Intra-articular Injection of Growth Hormone and Hyaluronic Acid in Rabbit Model of Collagenase-induced Osteoarthritis

    OpenAIRE

    Kim, Sang Beom; Kwon, Dong Rak; Kwak, Hyun; Shin, Yong Beom; Han, Hyun-jung; Lee, Jong Hwa; Choi, Seok Hwa

    2010-01-01

    In a rabbit model of collagenase-induced osteoarthritis, the additive effects of intra-articular recombinant human growth hormone (GH) administration to hyaluronic acid (HA) were evaluated. After intra-articular collagenase injection, mature New Zealand white rabbits (n=30) were divided into 3 groups. Group 1 (control rabbits) received once weekly intra-articular saline injections for 4 weeks. Group 2 rabbits received 6 mg HA injections, and group 3 rabbits were injected with 6 mg HA and 3 mg...

  3. Intra-articular temperatures of the knee in sports – An in-vivo study of jogging and alpine skiing

    Directory of Open Access Journals (Sweden)

    Cerulli Guiliano

    2008-04-01

    Full Text Available Abstract Background Up to date, no information exists about the intra-articular temperature changes of the knee related to activity and ambient temperature. Methods In 6 healthy males, a probe for intra-articular measurement was inserted into the notch of the right knee. Each subject was jogging on a treadmill in a closed room at 19°C room temperature and skiing in a ski resort at -3°C outside temperature for 60 minutes. In both conditions, temperatures were measured every fifteen minutes intra-articulary and at the skin surface of the knee. A possible influence on joint function and laxity was evaluated before and after activity. Statistical analysis of intra-articular and skin temperatures was done using nonparametric Wilcoxon's sign rank sum test and Mann-Whitney's-U-Test. Results Median intra-articular temperatures increased from 31.4°C before activity by 2.1°C, 4°C, 5.8°C and 6.1°C after 15, 30, 45 and 60 min of jogging (all p ≤ 0.05. Median intra-articular temperatures dropped from 32.2°C before activity by 0.5°C, 1.9°C, 3.6°C and 1.1°C after 15, 30, 45 and 60 min of skiing (all n.s.. After 60 minutes of skiing (jogging, the median intra-articular temperature was 19.6% (8.7% higher than the skin surface temperature at the knee. Joint function and laxity appeared not to be different before and after activity within both groups. Conclusion This study demonstrates different changes of intra-articular and skin temperatures during sports in jogging and alpine skiing and suggests that changes are related to activity and ambient temperature.

  4. Diagnostic Value of T-cell Interferon-γ Release Assays on Synovial Fluid for Articular Tuberculosis: A Pilot Study

    Institute of Scientific and Technical Information of China (English)

    Xin-He Cheng; Sai-Nan Bian; Yue-Qiu Zhang; Li-Fan Zhang; Xiao-Chun Shi; Bo Yang; Feng-Chun Zhang

    2016-01-01

    Background:Tuberculosis (TB) remains a major global public health challenge.Articular TB is an important form of extrapulmonary tuberculosis,and its diagnosis is difficult because of the low sensitivity of traditional methods.The aim of this study was to analyze the diagnostic value of T-SPOT.TB on synovial fluid for the diagnosis of articular TB.Methods:Patients with suspected articular TB were enrolled consecutively between August 2011 and December 2015.T-SPOT.TB was performed on both synovial fluid mononuclear cells (SFMCs) and peripheral blood mononuclear cells (PBMCs).The final diagnosis of articular TB was independent of the T-SPOT.TB result.The diagnostic sensitivity,specificity,predictive value,and likelihood ratio of T-SPOT.TB on SFMCs and PBMCs were analyzed.Results:Twenty patients with suspected articular TB were enrolled.Six were diagnosed with articular TB,and 14 patients were diagnosed with other diseases.Sensitivity and specificity were 83% and 86% for T-SPOT.TB on SFMCs,and 67% and 69% for T-SPOT.TB on PBMCs,respectively.The positive predictive value (PPV) and negative predictive value (NPV) of T-SPOT.TB on SFMCs were 71% and 92%,respectively.The PPV and NPV were 50% and 82% for T-SPOT.TB on PBMCs.Conclusion:Sensitivity,specificity,and NPV of T-SPOT.TB on SFMCs appeared higher than that on PBMCs,indicating that T-SPOT.TB on SFMCs might be a rapid and accurate diagnostic test for articular TB.

  5. Assessment of range of motion and muscular shortening in female flamenco dancers. Valoración de las amplitudes articulares y acortamientos musculares en bailaoras de flamenco.

    Directory of Open Access Journals (Sweden)

    José L. Costa Sepúlveda

    2011-12-01

    Full Text Available The purpose of the study was to assess flexibility and range of motion in flamenco dancers of Cádiz, Sevilla and Jaén (Spain through a tests battery. The study population comprised 37 healthy flamenco dancers (25 ± 7,2 years, 1,6 ± 0,5 m y 56 ± 7,6 Kg. They performed a range of flexibility and motion tests (i.e. Kendall test, Nachlas test. Results has shown that there is muscle shortening in most of the tests that flamenco dancers has passed. We conclude that there are not many articles on physiological and fitness aspects of dance and we think that it is necessary a specific physical dancer training, to prevent injuries and to extend dancers life.El objetivo de este estudio es la valoración de la flexibilidad muscular y la amplitud articular en bailaoras de flamenco de la provincia de Cádiz, Sevilla y Jaén, a través de una batería de tests. En el estudio participaron 37 bailaoras de danza flamenca de 25 ± 7,2 años, con una altura con valores de 1,6 ± 0,5 m y 56 ± 7,6 Kg de peso. La batería está compuesta de las siguientes pruebas que se realizarán a través del protocolo de actuación especificado: Prueba de rotadores internos y aductores del hombro, Prueba de Kendall, Prueba de Diagonal Posterior, Prueba de Nachlas, Prueba de Ridge, Prueba de Flexión de cadera con rodilla en extensión, Prueba de Thomas y Prueba de Elongación de los flexores plantares. Los resultados demuestran que existen acortamientos en diferente musculatura implicada como los rotadores internos y aductores del hombro, dorsal ancho, pectoral mayor, redondo mayor, cintura escapular, psoas-ilíaco, recto anterior del muslo y sóleo. Concluir con la escasa existencia de artículos relacionados con la valoración de la condición física de bailarines de cualquier modalidad de danza y con la necesaria aplicación de un entrenamiento planificado complementario con una propuesta de ejercicios de mejora de la musculatura implicada y, así, poder prevenir futuras

  6. Diagnóstico y propuesta para una gestión integral del riesgo en el Valle de Aburrá: red riesgos.

    OpenAIRE

    Aristizábal, Edier; VARGAS, RICHARD; Mesa Sánchez, Óscar José

    2008-01-01

    A partir del análisis de la situación organizacional de las diferentes instituciones en el Valle de Aburrá frente a la reducción de desastres y emergencias asociadas con los fenómenos de origen natural y antrópico, el Área Metropolitana del Valle de Aburrá, como autoridad ambiental y entidad planificadora, hace una propuesta que pretende articular todas las entidades, organizaciones e instituciones públicas y privadas hacia una gestión integral del riesgo, y la cual denominó: Red para la Gest...

  7. Estudio del Helicobacter pylori en el Perú

    Directory of Open Access Journals (Sweden)

    Alberto Ramírez Ramos

    2002-10-01

    Full Text Available El presente es el resultado del trabajo que diversos investigadores realizaron sobre la infección del H. pylori en el Perú. Epidemiología: notamos que la infección se adquiere a edades tempranas de la vida, siendo probablemente la vía oral-fecal y por el agua la forma de transmisión más importante. La prevalencia de la infección por H. pylori en los niveles socioeconómicos bajos es la misma en la costa, sierra y selva, manteniéndose estacionaria, a diferencia de los niveles medio y alto donde está disminuyendo (fenómeno asociado a la disminución de úlcera péptica y adenocarcinoma de estómago No existe evidencia que permita afirmar que ciertas razas tienen mayor predisposición para adquirir la infección, pero se ha notificado que en la población de altura es más frecuente la prevalencia de gastritis crónica atrófica que en la que habita a nivel del mar. Microbiología, biología molecular y patología: las cepas del H. pylori predominantes en el Perú son similares a las de España y Europa, pero muy diferentes a las encontradas en China y Japón. La gastritis crónica superficial, gastritis crónica profunda y gastritis crónica atrófica son diferentes estadios evolutivos de la lesión inflamatoria producida por la bacteria en el estómago. Diagnóstico: la biopsia de estómago utilizando coloración con plata es el patrón de oro. La combinación de dos métodos también puede ser útil. Tratamiento: los esquemas triples producen erradicación mayor al 80% los esquemas dobles y la monoterapia son insuficientes: la tasa de recaída postratamiento es alta y la mayoría por una cepa diferente.

  8. Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection

    Directory of Open Access Journals (Sweden)

    Chen Z

    2013-10-01

    Full Text Available Zhipeng Chen,* Juan Chen,* Li Wu, Weidong Li, Jun Chen, Haibo Cheng, Jinhuo Pan, Baochang CaiDepartment of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China*These authors contributed equally to this workObjective: To evaluate the potential of hyaluronic acid (HA-coated bovine serum albumin nanoparticles (BSANPs as a novel chondrocyte-targeting drug-delivery nanomedicine.Methods: The HA-BSANPs were characterized by dynamic light scattering, transmission electron microscopy, differential scanning calorimetry, and X-ray diffraction. Fluorescence imaging was used to visualize the distribution of nanoparticles after intra-articular injection. The chondrocyte-targeting efficiency and cellular uptake mechanism of HA-BSANPs were investigated using endocytic inhibitors.Results: HA-BSANPs were successfully prepared with HA coating the surface and amorphous drug in the core. Compared with BSANPs, HA-BSANPs exhibited improved uptake by chondrocytes through a receptor-mediated active uptake mechanism. The endocytosis process of BSANPs and HA-BSANPs involved clathrin-mediated endocytosis, caveolae-mediated endocytosis, and macropinocytosis. No apparent thickening or hyperplasia of the synovium was observed in either BSANPs or HA-BSANPs. The HA-BSANPs could reside in the articular cavity of rats for more than 14 days, which was significantly longer than BSANPs.Conclusion: HA-BSANPs are a promising carrier for articular-related diseases due to elongated articular residence and improved chondrocytic accumulation.Keywords: chondrocyte, intra-articular injection, hyaluronic acid, BSA, nanoparticles

  9. The effects of proteoglycan and type II collagen on T1rho relaxation time of articular cartilage

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Won Seok; Yoo, Hye Jin; Hong, Sung Hwan; Choi, Ja Young [Dept. of Radiology and Institute of Radiation Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul (Korea, Republic of)

    2015-02-15

    To evaluate the effects of proteoglycan and type II collagen within articular cartilage on T1rho relaxation time of articular cartilage. This study was exempted by the institutional and animal review boards, and informed consent was not required. Twelve porcine patellae were assigned to three groups of control, trypsin-treated (proteoglycan-degraded), or collagenase-treated (collagen-degraded). The T1rho images were obtained with a 3 tesla magnetic resonance imaging scanner with a single loop coil. Statistical differences were detected by analysis of variance to evaluate the effects of the enzyme on T1rho relaxation time. Safranin-O was used to stain proteoglycan in the articular cartilage and immunohistochemical staining was performed for type II collagen. Mean T1rho values of the control, trypsin-treated, and collagenase-treated groups were 37.72 +/- 5.82, 57.53 +/- 8.24, and 45.08 +/- 5.31 msec, respectively (p < 0.001). Histology confirmed a loss of proteoglycan and type II collagen in the trypsin- and collagenase-treated groups. Degradation of proteoglycans and collagen fibers in the articular cartilage increased the articular cartilage T1rho value.

  10. Culturas del Mundo

    DEFF Research Database (Denmark)

    Benwell, Ann Fenger; Costa, Alberto; Waehle, Espen

    2006-01-01

    ’Culturas del mundo. Colecciones del Museo Nacional de Dinamarca’ with Ann Fenger Benwell in Culturas del Mundo. Colecciones del Museo Nacional de Dinamarca, ed. Silvia Sauquet, Fundación "la Caixa", Barcelona 2006, pp. 31-39......’Culturas del mundo. Colecciones del Museo Nacional de Dinamarca’ with Ann Fenger Benwell in Culturas del Mundo. Colecciones del Museo Nacional de Dinamarca, ed. Silvia Sauquet, Fundación "la Caixa", Barcelona 2006, pp. 31-39...

  11. The Role of Computed Tomography in Evaluating Intra-Articular Distal Humerus Fractures.

    Science.gov (United States)

    Nolan, Betsy M; Sweet, Stephan J; Ferkel, Eric; Udofia, Aniebet-Abasi; Itamura, John

    2015-09-01

    Computed tomography (CT) is often used to evaluate intra-articular distal humerus fracture patterns, but it increases radiation exposure and cost. We conducted a study to determine the effect of adding CT evaluation to plain radiographic evaluation on the classification of, and treatment plans for, intra-articular distal humerus fractures. Nine blinded orthopedic surgeons evaluated 30 consecutive fractures for classification and surgical approach. Evaluations were performed first using plain radiographs and then again using the same radiographs plus CT images. Statistical analysis was performed using the κ correlation coefficient and Cramer V testing. We hypothesized that adding CT images to plain radiographs would change the classification and treatment of these fractures and would improve interobserver agreement on classification and treatment. Intraobserver reliability (Cramer V) was fair (.393) for classification and moderate (.426) for treatment. Interobserver reliability (Cohen κ) did not improve with CT: For classification, κ was .21 without CT and .20 with CT; for treatment, κ was .28 without CT and .27 with CT. When classifying the fractures, attending surgeons chose the multiplanar fracture pattern 25.6% of the time without CT, and remained consistent at 23.3% with CT. Trainees chose this fracture pattern much less often without CT than with CT. Use of CT changed the treatment for multiplanar fractures (73.7% lateral approach vs 51.9% posterior approach with olecranon osteotomy). When added to plain radiographic evaluation, CT evaluation changes classification and treatment plans. Interobserver reliability did not improve. Less experienced surgeons were more likely to identify multiplanar fracture patterns with use of CT. We recommend performing CT for all intra-articular distal humerus fractures.

  12. Intra-articular hip injection: does pain relief correlate with radiographic severity of osteoarthritis?

    Energy Technology Data Exchange (ETDEWEB)

    Deshmukh, Ajit J.; Rodriguez, Jose A. [North Shore LIJ Lenox Hill Hospital, Department of Orthopaedic Surgery, New York, NY (United States); Panagopoulos, Georgia [North Shore LIJ Lenox Hill Hospital, Department of Research, New York, NY (United States); Alizadeh, Ahmadreza; Klein, Devon A. [North Shore LIJ Lenox Hill Hospital, Department of Radiology, New York, NY (United States)

    2011-11-15

    Intra-articular injection is being used widely for both diagnostic and therapeutic purposes in the hip. However, its efficacy is not always predictable in patients with hip osteoarthritis (OA). The purpose of this study was to determine whether the degree of radiographic severity of OA was predictive of the response to intra-articular injection of local anesthetic with corticosteroid and to determine the relationship between immediate pain relief resulting from the anesthetic and delayed pain relief resulting from corticosteroid administration. This retrospective study included 217 patients (220 injections) with diagnosis of hip OA who underwent a fluoroscopically guided therapeutic hip injection of local anesthetic and corticosteroid. Hip radiographs were scored using the Kellgren-Lawrence scale. Immediate and delayed pain relief was documented using a visual analog scale. Logistic regression analysis was performed to investigate whether age, gender or radiographic severity of OA were independent predictors of pain relief. Degree of agreement between immediate and delayed response was assessed with the kappa coefficient. Immediate pain relief was reported in 68.2% of hips and delayed relief in 71.4% of hips. A high level of agreement was observed between immediate and delayed pain relief (kappa = 0.80, p < 0.001). 94% of patients reporting immediate relief also reported relief 2 weeks later. Univariate and multivariate analysis revealed that neither gender nor age was related to immediate or delayed pain relief. Only severity of OA (based on radiographic analysis) was observed to be predictive of pain relief. Pain relief following intra-articular hip injection correlated with radiographic severity of OA. This intervention may be of therapeutic and prognostic value in patients awaiting hip arthroplasty. (orig.)

  13. Arthroscopic optical coherence tomography provides detailed information on articular cartilage lesions in horses.

    Science.gov (United States)

    te Moller, N C R; Brommer, H; Liukkonen, J; Virén, T; Timonen, M; Puhakka, P H; Jurvelin, J S; van Weeren, P R; Töyräs, J

    2013-09-01

    Arthroscopy enables direct inspection of the articular surface, but provides no information on deeper cartilage layers. Optical coherence tomography (OCT), based on measurement of reflection and backscattering of light, is a diagnostic technique used in cardiovascular surgery and ophthalmology. It provides cross-sectional images at resolutions comparable to that of low-power microscopy. The aim of this study was to determine if OCT is feasible for advanced clinical assessment of lesions in equine articular cartilage during diagnostic arthroscopy. Diagnostic arthroscopy of 36 metacarpophalangeal joints was carried out ex vivo. Of these, 18 joints with varying degrees of cartilage damage were selected, wherein OCT arthroscopy was conducted using an OCT catheter (diameter 0.9 mm) inserted through standard instrument portals. Five sites of interest, occasionally supplemented with other locations where defects were encountered, were arthroscopically graded according to the International Cartilage Repair Society (ICRS) classification system. The same sites were evaluated qualitatively (ICRS classification and morphological description of the lesions) and quantitatively (measurement of cartilage thickness) on OCT images. OCT provided high resolution images of cartilage enabling determination of cartilage thickness. Comparing ICRS grades determined by both arthroscopy and OCT revealed poor agreement. Furthermore, OCT visualised a spectrum of lesions, including cavitation, fibrillation, superficial and deep clefts, erosion, ulceration and fragmentation. In addition, with OCT the arthroscopically inaccessible area between the dorsal MC3 and P1 was reachable in some cases. Arthroscopically-guided OCT provided more detailed and quantitative information on the morphology of articular cartilage lesions than conventional arthroscopy. OCT could therefore improve the diagnostic value of arthroscopy in equine orthopaedic surgery. PMID:23810744

  14. An intra-articular ganglion cyst in a patient with juvenile idiopathic arthritis.

    Science.gov (United States)

    Deng, Donna Y; Yee, Keolamau; Burkhalter, William; Okimoto, Kelley Chinen; Kon, Kevin; Kurahara, David K

    2014-01-01

    We report an intra-articular ganglion cyst (IAGC) presenting as knee pain and a mass in a patient with longstanding Juvenile Idiopathic Arthritis (JIA). We could not find a similar case of an IAGC occurring in the knee of JIA patients in the literature. IAGC may need to be included as a possibility in patients with inflammatory arthritis with new-onset knee pain, especially in those with a palpable mass. MRI was useful in distinguishing IAGC from more worrisome causes of a knee mass. Orthopedic input was helpful in diagnosis and treatment. In addition, methotrexate therapy was effective in bringing about a long-lasting remission.

  15. Functional bracing for comminuted extra-articular fractures of the distal third of the humerus.

    Science.gov (United States)

    Sarmiento, A; Horowitch, A; Aboulafia, A; Vangsness, C T

    1990-03-01

    From 1982 to 1987 we treated 85 extra-articular comminuted distal third humeral fractures in adults with prefabricated plastic braces. Of these, 15% were open fractures and 18% had initial peripheral nerve injury. On average, the sleeve was applied 12 days after injury and used for 10 weeks. There was 96% union, with no infections. All nerve injuries resolved or were improving at the latest examination. At union there was varus deformity averaging 9 degrees in 81% of patients, but loss of range of movement was minimal and functional results were good.

  16. Doença articular degenerativa da articulação temporomandibular

    OpenAIRE

    Carvalho, Joana Alexandra Alves de

    2009-01-01

    Monografia apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Licenciada em Medicina Dentária Este trabalho foi desenvolvido com o intuito de abordar diversas questões relacionadas com o desenvolvimento da Doença Articular Degenerativa (DAD) ao nível da Articulação Temporomandibular (ATM). Os eventos moleculares que estão na base da patogénese da DAD da ATM e as artralgias associadas são complexos. Modelos contemporâneos sugerem que excessivas ...

  17. Hypersensitivity to mechanical and intra-articular electrical stimuli in persons with painful temporomandibular joints

    DEFF Research Database (Denmark)

    Ayesh, Emad; Jensen, Troels Staehelin; Svensson, P

    2007-01-01

    This study tested whether persons with TMJ arthralgia have a modality-specific and site-specific hypersensitivity to somatosensory stimuli assessed by quantitative sensory tests (QST). Forty-three healthy persons and 20 with TMJ arthralgia participated. The QST consisted of: sensory and pain...... detection thresholds and summation threshold to intra-articular electrical stimulation, tactile and pin-prick sensitivity in the TMJ area, pressure-pain threshold and tolerance on the lateral side of the TMJ and on the finger. Persons with TMJ arthralgia had lower pain detection and summation thresholds (P...

  18. Anterolateral Extra-articular Soft Tissue Reconstruction in Anterolateral Rotatory Instability of the Knee.

    Science.gov (United States)

    Kernkamp, Willem A; van de Velde, Samuel K; Bakker, Eric W P; van Arkel, Ewoud R A

    2015-12-01

    Anterolateral rotatory instability (ALRI) occurs after injury to the anterior cruciate ligament (ACL) and the anterolateral structures of the knee. We present a technique for anterolateral extra-articular soft-tissue (ALES) reconstruction of the knee that can be used in revision ACL reconstruction cases, cases of persistent ALRI after adequate ACL reconstruction, and cases with severe ALRI after primary ACL rupture. The surgeon performs ALES reconstruction with a strip of iliotibial tract autograft while respecting the anatomic origin and insertion of the anterolateral ligament. The purpose of this reconstruction is to restore the normal anterolateral rotatory stability of the knee in ALES-deficient patients. PMID:27284525

  19. Efeitos do resfriamento e aquecimento articular no desempenho funcional do ombro

    OpenAIRE

    Dayane de Oliveira Estevam; Marcelo Lima de Oliveira; Marcelo Lourenço da Silva; Leonardo César Carvalho; Daniel Ferreira Moreira Lobato

    2015-01-01

    INTRODUÇÃO: Um dos recursos mais utilizados na reabilitação de lesões musculoesqueléticas é a termoterapia por subtração de calor (crioterapia), enquanto a termoterapia por adição de calor é considerada o procedimento mais antigo de reabilitação física. Entretanto, há poucas evidências que tenham investigado os efeitos desses recursos sobre o desempenho de membros superiores. OBJETIVO: Comparar o efeito do resfriamento e do aquecimento articular sobre o desempenho funcional do membro superior...

  20. Nitric oxide compounds have different effects profiles on human articular chondrocyte metabolism

    OpenAIRE

    de Andrés, María C.; Maneiro, Emilia; Martín, Miguel A.; Arenas, Joaquín; Francisco J Blanco

    2013-01-01

    Introduction The pathogenesis of osteoarthritis (OA) is characterized by the production of high amounts of nitric oxide (NO), as a consequence of up-regulation of chondrocyte-inducible nitric oxide synthase (iNOS) induced by inflammatory cytokines. NO donors represent a powerful tool for studying the role of NO in the cartilage in vitro. There is no consensus about NO effects on articular cartilage in part because the differences between the NO donors available. The aim of this work is to com...

  1. MRI findings in injured articular cartilage of the knee correlated with surgical findings

    Institute of Scientific and Technical Information of China (English)

    LI Xiao-ming; PENG Wen-jia; WU Hua; Kacher Daniel; XIA Li-ming; AI Fei; LI Feng; XIONG Wei

    2009-01-01

    Background There is a strong need for quick noninvasive diagnostic technique that can give a valid estimate of the status of the cartilage reliably,discriminating intact cartilage from various grades of impaired cartilage.The goal of this study was to assess the incidence of knee cartilage injuries and compare the accuracy of two-dimension spin echo(2D SE)and fast spin echo(FSE)(conventional MRI),three-dimensional spoiled gradient echo(3D SPGR),three-dimensional fast imaging employing steady state acquisition(3D FIESTA)MR imaging sequences with surgical examination of the articular cartilage.Methods One hundred and thirty-eight knees with history of knee trauma received conventional MRI,3D SPGR and 3D FIESTA MRI examination before surgery,and surgical examination of articular cartilage was used as reference standard.A modified version of the Noyes classification system was applied for the evaluation of the lateral femoral condyle(LFC),medial femoral condyle(MFC),lateral tibial plateau(LTP),medial tibial plateau(MTP),trochlea and patella.The incidence and distributions of different injured grades at different articular surfaces of knee were assessed.A series of assessment indeces of 3D SPGR,3D FIESTA,and the combination of the conventional MRI and 3D SPGR imaging were calculated.Results The incidence of cartilage defects(grade 2 to 4)was 22%(183/828),according to surgical examination.Grade 3 and 4 lesions were absent at the medial tibial plateau.The rates of exact match between the grading results of different MRI procedures and surgical examination were 49% of 3D SPGR,61% of 3D FIESTA,and 82% of the combination of 3D SPGR and conventional MRI.Also,the combination of 3D SPGR and conventional MR imaging provided the highest sensitivity,specificity,accuracy,positive and negative predictive values,at 71%,97%,90%,90% and 90%,respectively.Conclusions For all the articular surfaces of the traumatic knees,about one fifth(22%)were cartilage defects.Both 3D SPGR and 3D

  2. Arthroscopic Excision of Juxta-articular Osteoid Osteoma of the Calcaneum

    Science.gov (United States)

    Tauheed, Mohammed; Korula, Ravi Jacob; Shankarnarayanan, Sriram

    2016-01-01

    Osteoid osteoma of the foot is a rare condition particularly of the calcaneum. This condition is difficult to diagnose and is more difficult to treat particularly if it involves deeper part of the joints. We present an arthroscopic technique to deal with a case of juxta-articular osteoid osteoma of the calcaneum using two portals: the anterolateral portal for instrumentation and the anterior anterolateral portal for visualization of the subtalar joint. Because this approach is minimally invasive, it offers early recovery and reduced morbidity compared with the conventional techniques. PMID:27073769

  3. Effect of low-power helium-neon laser irradiation on 13-week immobilized articular cartilage of rabbits.

    Science.gov (United States)

    Bayat, Mohammad; Ansari, Anayatallah; Hekmat, Hossien

    2004-09-01

    Influence of low-power (632.8 nm, Helium-Neon, 13 J/cm2, three times a week) laser on 13-week immobilized articular cartilage was examined with rabbits knee model. Number of chondrocytes and depth of articular cartilage of experimental group were significantly higher than those of sham irradiated group. Surface morphology of sham-irradiated group had rough prominences, fibrillation and lacunae but surface morphology of experimental group had more similarities to control group than to sham irradiated group. There were marked differences between ultrastructure features of control group and experimental group in comparison with sham irradiated group. Low-power Helium-Neon laser irradiation on 13-week immobilized knee joints of rabbits neutrilized adverse effects of immobilization on articular cartilage.

  4. FIFTH LUMBAR VERTEBRA ASSOCIATED WITH ABSENCE OF SPINOUS PROCESS, LAMINAE AND INFERIOR ARTICULAR PROCESSES. – CASE REPORT

    Directory of Open Access Journals (Sweden)

    Prathap Kumar

    2013-09-01

    Full Text Available Background: The vertebral disorders are the ones which lead to disability and lot of health problems. Since the lumbar part of the vertebral column is the main weight bearing and weight transmitting region, if there is a defective development, the area for muscle attachment and the strong bony structure for the transmission of weight would be missing leading to instability at an early age. In the present case dry and processed fifth lumbar vertebra, of unknown sex which presented the features with absence of spinous process, laminae and the inferior articular processes on both sides which were obtained for teaching the medical undergraduate students in M.S.Ramaiah Medical College, Bangalore. There was absence of spinous process, laminae and the inferior articular processes of fifth lumbar vertebra leading to a wide spina bifida with absence of laminae, inferior articular processes on both sides and spinous process of fifth lumbar vertebra which could be a developmental anomaly.

  5. CLINICAL OBSERVATION ON THE THERAPEUTIC EFFECT OF ACUPUNCTURE PLUS MANUAL REPOSITION FOR TREATMENT OF ACUTE LUMBAR VERTEBRAL ARTICULAR DYSKINESIA

    Institute of Scientific and Technical Information of China (English)

    骆钧梵

    2003-01-01

    Objective: To observe the therapeutic effect of acupuncture plus manual reposition for treatment of acute lumbar vertebral articular dyskinesia for choosing a better remedy. Methods: 66 cases of acute lumbar vertebral articular dyskinesia were randomly divided into acupuncture plus manual reposition group (treatment group, n= 33) and routine manual reposition group (control group, n = 33). Yaotong-point was punctured, when, the patient was asked to move his or her waist simultaneously. Results: After one session of treatment, of the two 33 cases in treatment and control groups, 28 (84.85%) and20 (60.61%) were cured, 4 (12.12%) and 9 (27.27%) were improved, and 1 (3.03%) and 4 (12.12%) failed in the treatment. The therapeutic effect of treatment group was significantly superior to that of control group ( P< 0.05). Conclusion: Acupuncture combined with manual reposition is apparently superior to simple routine manual reposition in relieving acute lumbar vertebral articular dyskinesia.

  6. An evaluation of MRI in the diagnosis of occult peri-articular bone injury of knee joint

    International Nuclear Information System (INIS)

    Objective: An evaluation of MRI in the diagnosis of occult peri-articular bone injury. Methods: Thirty-one patients, in total thirty-three joints, underwent radiography and MRI. MRI sequences included FSE T1WI, FSE T2WI, T2WI/SPIR, PDWI/SPIR and 3D/WATSc/FPE/T1WI. Results: Abnormities were found in thirty-three knee joints. Bone bruises were found in 20 knees, occult fractures in 8 and articular cartilage or osteochondral fractures in 5. T2WI/SPIR was successful in demonstrating the bone bruise. PDWI/SPIR provided good images of the occult fracture, while 3D/WATSc/FFE/T1WI was the best sequence to demonstrate the cartilage fracture. Conclusion: MRI provides vivid images of the peri-articular occult injury of knee. Various MRI sequences have respective advantages in differentiating the nature of occult lesions and should be combined in clinical application. (authors)

  7. Intra-articular synovial sarcoma treated with a transfemoral amputation: a case report and review of the literature.

    Science.gov (United States)

    Gresswell, Steven D; Corsini, Anthony A; Balsamo, Luke H; Miles, Edward F

    2013-08-01

    A case of monophasic intra-articular synovial sarcoma in the right knee of a 39-year-old active duty serviceman treated with a transfemoral amputation is presented. The patient was evaluated for right knee pain and fullness. After further workup, the patient underwent computed tomography guided biopsy, with the tissue specimen consistent with intra-articular synovial sarcoma. The patient elected for a transfemoral amputation rather than limb or joint-sparing surgery. The gross specimen measured 3.5 × 3.0 × 1.7 cm in the posteromedial knee. No metastatic lesions were seen on positron emission tomography-computed tomography. Chemotherapy and radiation therapy have not been utilized. The transfemoral amputation adds to the uniqueness of this report and is discussed with a review of the multimodality treatment toward intra-articular synovial sarcoma in prior published literature.

  8. Chondrogenic Differentiation of Human Adipose-Derived Stem Cells: A New Path in Articular Cartilage Defect Management?

    Directory of Open Access Journals (Sweden)

    Jan-Philipp Stromps

    2014-01-01

    Full Text Available According to data published by the Centers for Disease Control and Prevention, over 6 million people undergo a variety of medical procedures for the repair of articular cartilage defects in the U.S. each year. Trauma, tumor, and age-related degeneration can cause major defects in articular cartilage, which has a poor intrinsic capacity for healing. Therefore, there is substantial interest in the development of novel cartilage tissue engineering strategies to restore articular cartilage defects to a normal or prediseased state. Special attention has been paid to the expansion of chondrocytes, which produce and maintain the cartilaginous matrix in healthy cartilage. This review summarizes the current efforts to generate chondrocytes from adipose-derived stem cells (ASCs and provides an outlook on promising future strategies.

  9. Efecto de la movilización articular sobre la amplitud del Reflejo H en personas con espasticidad

    Directory of Open Access Journals (Sweden)

    Julio Ernesto Pérez Parra

    2011-08-01

    Full Text Available Objective: To determine the effect of ankle joint mobilization on the H reflex amplitude of the soleus muscle in people with spasticity. Materials and methods: A quasi-experimental study with crossover design and simple masking was conducted in 24 randomized subjects to initiate the control or experimental group. Traction and rhythmic oscillation were applied for five minutes to the ankle joint. H wave amplitude changes of Hoffmann reflex (electrical equivalent of the monosynaptic spinal reflex was assessed, stimulating the tibial nerve at the level of the popliteal fossa and recording in the soleusmuscle. In each subject 12 measurements were taken: basalrate, during and after mobilization. Changes in H reflex amplitudewere calculated in relation to basal measurement. For each measurement a hypothesis test was performed (Student t test. Results: In groups of patients with brain injury and incomplete spinal cord injury, a significant difference was found between measurements of both studies, concerning variation in H reflex amplitude during the application of joint mobilization techniques, with a decrease in the experimental group and an increase in the control group. In contrast, no significant differences were found after mobilization therapy. Patients with complete spinal cord injuryshowed no significant differences in any measurements. Conclusion: Wedemonstrate the effectiveness of joint mobilization in the decreaseof H reflex amplitude in patients with brain injury or incompletespinal cord injury during the mobilization maneuver, but no residualeffect after completion of the trial. This research showed noevidence regarding excitability reduction in complete spinal cordinjury. We suggest that therapeutic interventions to decrease muscletone based on the joint mobilization should be reconsidered.

  10. Efecto de la movilización articular sobre la amplitud del Reflejo H en personas con espasticidad

    OpenAIRE

    Julio Ernesto Pérez Parra; Claudia Patricia Henao Lema

    2011-01-01

    Objective: To determine the effect of ankle joint mobilization on the H reflex amplitude of the soleus muscle in people with spasticity. Materials and methods: A quasi-experimental study with crossover design and simple masking was conducted in 24 randomized subjects to initiate the control or experimental group. Traction and rhythmic oscillation were applied for five minutes to the ankle joint. H wave amplitude changes of Hoffmann reflex (electrical equivalent of the monosynaptic spinal refl...

  11. Competencias emocionales en el proceso de formación del docente de Educación Infantil

    OpenAIRE

    Pegalajar Palomino, María del Carmen; López Hernáez, Lara

    2015-01-01

    El proceso de formación inicial del docente debe plantear el desarrollo de competencias emocionales, pues resultan cruciales para dotar de calidad la enseñanza del sistema educativo. De este modo, se deben articular acciones tendentes a facilitar el desarrollo de habilidades para que el docente tome consciencia de sí mismo, a la vez que maneje y reconozca tanto sus emociones y motivaciones como las de los otros. Esta investigación pretende evaluar el nivel de autorrealización y crecimiento pe...

  12. Clinical efficacy of intra-articular injections in knee osteoarthritis: a prospective randomized study comparing hyaluronic acid and betamethasone

    Directory of Open Access Journals (Sweden)

    Trueba Davalillo CA

    2015-01-01

    Full Text Available Cesáreo Ángel Trueba Davalillo,1,2 Cesáreo Trueba Vasavilbaso,2 José Mario Navarrete Álvarez,2 Pilar Coronel Granado,3 Ozcar Alejandro García Jiménez,2 Mercedes Gimeno del Sol,3 Félix Gil Orbezo2 1School of Medicine (UNAM, México DF, Mexico; 2Orthopedic Service, Hospital Español de México, México DF, Mexico; 3Scientific Department, TEDEC-MEIJI FARMA,SA, Alcalá de Henares, Spain Background: Osteoarthritis (OA is the most common joint disease and leading cause of disability. Intra-articular (IA administration of hyaluronic acid (HA or corticosteroids (CS have been previously studied, though using insufficient number of patients or short follow-up periods.Objective: We evaluate HA and CS in patients with knee OA in terms of clinical efficacy over 12 months.Methods: We used a prospective, randomized study with parallel groups. Randomized patients received IA injections of HA or betamethasone (BM. The primary outcomes were improvement in pain using Visual Analog Scale and function in the Western Ontario and McMaster University Osteoarthritis Index (Likert scale. Follow-up visits were scheduled at 3 months, 6 months, 9 months, and 12 months.Results: A total of 200 patients were included. Pain was significantly reduced in both groups at the first follow-ups. At 12 months, the mean pain reduction in the HA group was 33.6% (95% CI: 31.1–36.1 compared to 8.2% (95% CI: 5.2–11.1 in BM (P<0.0001. Function improvement was higher in HA through every visit, and mean improvement at 12 months was 47.5% (95% CI: 45.6–49.3 in HA patients vs 13.2% (95% CI: 11.4–14.9 in the BM group (P<0.0001. All patients from both groups achieved the Minimal Clinically Important Improvement (MCII for both pain and function up to 6 months. At 9 months and 12 months, the MCII figures were higher in HA group with ≥80% compared to ≤10% in BM group (P<0.0001. Adverse reactions were rare and related to the administration procedure.Conclusion: Both treatments

  13. A randomized controlled trial of nonoperative treatment versus open reduction and internal fixation for stable, displaced, partial articular fractures of the radial head: The RAMBO trial

    NARCIS (Netherlands)

    W. Bruinsma (Wendy); I.F. Kodde (Izaäk Frederik); R.-J. De Muinck Keizer (Robert-Jan); P. Kloen (Peter); A. Lindenhovius (Anneluuk); J.P.A.M. Vroemen (Jos); R. Haverlag (Robert); M.P.J. van den Bekerom (Michel); H.W. Bolhuis (Hugo); P. Bullens (Pieter); S.A.G. Meylaerts (Sven); P. van der Zwaal (Peer); E.P. Steller; G.S. Hageman (Gregory); D. Ring (David); D. den Hartog (Dennis); E.R. Hammacher (Eric); G. King (Graham); G. Athwal (George); K. Faber (Ken); D. Drosdowech (Darren); R. Grewal (Ruby); J.C. Goslings (Carel); N.W.L. Schep (Niels); D. Eygendaal (Denise)

    2014-01-01

    textabstractBackground: The choice between operative or nonoperative treatment is questioned for partial articular fractures of the radial head that have at least 2 millimeters of articular step-off on at least one radiograph (defined as displaced), but less than 2 millimeter of gap between the frag

  14. Exploring reasons for the observed inconsistent trial reports on intra-articular injections with hyaluronic acid in the treatment of osteoarthritis

    DEFF Research Database (Denmark)

    Johansen, Mette; Bahrt, Henriette; Altman, Roy D;

    2016-01-01

    OBJECTIVE: The aim was to identify factors explaining inconsistent observations concerning the efficacy of intra-articular hyaluronic acid compared to intra-articular sham/control, or non-intervention control, in patients with symptomatic osteoarthritis, based on randomized clinical trials (RCTs)...

  15. Articular Cartilage Evaluation After TruFit Plug Implantation Analyzed by Delayed Gadolinium-Enhanced MRI of Cartilage (dGEMRIC)

    NARCIS (Netherlands)

    Bekkers, J.E.J.; Bartels, L.W.; Vincken, K.L.; Dhert, W.J.A.; Creemers, L.B.; Saris, D.B.F.

    2013-01-01

    Background: Quantitative MRI of articular cartilage has rapidly developed in recent years and provides the clinician with a noninvasive tool to determine the biological consequence of an intervention. Purpose: To evaluate the quality of intra-articular cartilage, using the dGEMRIC scanning techniqu

  16. Intra-articular fibroma of tendon sheath in a knee joint associated with iliotibial band friction syndrome.

    Science.gov (United States)

    Ha, Dong-Ho; Choi, Sunseob; Kim, Soo-Jin; Lih, Wang

    2015-01-01

    Iliotibial band (ITB) friction syndrome is a common overuse injury typically seen in the active athlete population. A nodular lesion on the inner side of the ITB as an etiology or an accompanying lesion from friction syndrome has been rarely reported. A 45-year-old male presented with recurrent pain and a movable nodule at the lateral joint area, diagnosed as ITB friction syndrome. The nodule was confirmed as a rare intra-articular fibroma of the tendon sheath (FTS) on the basis of histopathologic findings. We describe the MRI findings, arthroscopic and pathologic features, in this case of intra-articular FTS presenting with ITB friction syndrome.

  17. Intra-articular fibroma of tendon sheath in a knee joint associated with iliotibial band friction syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Ha, Dong Ho; Choi, Sun Seob; Kim, Soo Jin; Lih, Wang [Dong-A University Medical Center, Busan (Korea, Republic of)

    2015-02-15

    Iliotibial band (ITB) friction syndrome is a common overuse injury typically seen in the active athlete population. A nodular lesion on the inner side of the ITB as an etiology or an accompanying lesion from friction syndrome has been rarely reported. A 45-year-old male presented with recurrent pain and a movable nodule at the lateral joint area, diagnosed as ITB friction syndrome. The nodule was confirmed as a rare intra-articular fibroma of the tendon sheath (FTS) on the basis of histopathologic findings. We describe the MRI findings, arthroscopic and pathologic features, in this case of intra-articular FTS presenting with ITB friction syndrome.

  18. Intra-articular fibroma of tendon sheath in a knee joint associated with iliotibial band friction syndrome.

    Science.gov (United States)

    Ha, Dong-Ho; Choi, Sunseob; Kim, Soo-Jin; Lih, Wang

    2015-01-01

    Iliotibial band (ITB) friction syndrome is a common overuse injury typically seen in the active athlete population. A nodular lesion on the inner side of the ITB as an etiology or an accompanying lesion from friction syndrome has been rarely reported. A 45-year-old male presented with recurrent pain and a movable nodule at the lateral joint area, diagnosed as ITB friction syndrome. The nodule was confirmed as a rare intra-articular fibroma of the tendon sheath (FTS) on the basis of histopathologic findings. We describe the MRI findings, arthroscopic and pathologic features, in this case of intra-articular FTS presenting with ITB friction syndrome. PMID:25598686

  19. Horizontal intra-articular patellar dislocation resulting in quadriceps avulsion and medial patellofemoral ligament tear: a case report.

    Science.gov (United States)

    Kramer, Dennis E; Simoni, Michael K

    2013-07-01

    Intra-articular patellar dislocations are rare. We present a 13-year-old boy who sustained a complete horizontal intra-articular patellar dislocation following blunt trauma to the flexed knee. Closed reduction was unsuccessful and open reduction indicated a repairable quadriceps avulsion and medial patellofemoral ligament tear. He is the youngest patient to sustain a quadriceps rupture and the only patient to sustain a medial patellofemoral ligament tear to date. His flexed knee and the horizontally positioned patella (seen on lateral radiograph) were indicative of a complete rotational injury with extensor mechanism involvement. Open reduction allowed for the repair of both injuries and a favorable outcome.

  20. Intra-articular fibroma of tendon sheath in a knee joint associated with iliotibial band friction syndrome

    International Nuclear Information System (INIS)

    Iliotibial band (ITB) friction syndrome is a common overuse injury typically seen in the active athlete population. A nodular lesion on the inner side of the ITB as an etiology or an accompanying lesion from friction syndrome has been rarely reported. A 45-year-old male presented with recurrent pain and a movable nodule at the lateral joint area, diagnosed as ITB friction syndrome. The nodule was confirmed as a rare intra-articular fibroma of the tendon sheath (FTS) on the basis of histopathologic findings. We describe the MRI findings, arthroscopic and pathologic features, in this case of intra-articular FTS presenting with ITB friction syndrome.

  1. Study of the collagen structure in the superficial zone and physiological state of articular cartilage using a 3D confocal imaging technique

    Directory of Open Access Journals (Sweden)

    Zheng Ming H

    2008-07-01

    Full Text Available Abstract Introduction The collagen structure in the superficial zone of articular cartilage is critical to the tissue's durability. Early osteoarthritis is often characterized with fissures on the articular surface. This is closely related to the disruption of the collagen network. However, the traditional histology can not offer visualization of the collagen structure in articular cartilage because it uses conventional optical microscopy that does not have insufficient imaging resolution to resolve collagen from proteoglycans in hyaline articular cartilage. This study examines the 3D collagen network of articular cartilage scored from 0 to 2 in the scoring system of International Cartilage Repair Society, and aims to develop a 3D histology for assessing early osteoarthritis. Methods Articular cartilage was visually classified into five physiological groups: normal cartilage, aged cartilage, cartilage with artificial and natural surface disruption, and fibrillated. The 3D collagen matrix of the cartilage was acquired using a 3D imaging technique developed previously. Traditional histology was followed to grade the physiological status of the cartilage in the scoring system of International Cartilage Repair Society. Results Normal articular cartilage contains interwoven collagen bundles near the articular surface, approximately within the lamina splendens. However, its collagen fibres in the superficial zone orient predominantly in a direction spatially oblique to the articular surface. With age and disruption of the articular surface, the interwoven collagen bundles are gradually disappeared, and obliquely oriented collagen fibres change to align predominantly in a direction spatially perpendicular to the articular surface. Disruption of the articular surface is well related to the disappearance of the interwoven collagen bundles. Conclusion A 3D histology has been developed to supplement the traditional histology and study the subtle changes in

  2. Deginerative changes of femoral articular cartilage in the knee : comparative study of specimen sonography and pathology

    International Nuclear Information System (INIS)

    To determine the sonographic findings of degenerative change in femoral articular cartilage of the knee by comparative study of specimen sonography and pathology. We obtained 40 specimens of cartilage of the femur (20 medial and 20 lateral condylar) from 20 patients with osteoarthritis of the knee who had undergone total knee replacement. The specimens were placed in a saline-filled container and sonography was performed using a 10-MHz linear transducer. Sonographic abnormalities were evaluated at the cartilage surface, within the cartilage, and at the bone-cartilage interface, and were compared with the corresponding pathologic findings. In addition, cartilage thickness was measured at a representative portion of each femoral cartilage specimen and was compared with the thickness determined by sonography. 'Dot' lesions, irregularity or loss of the hyperechoic line, were demonstrated by sonography at the saline-cartilage interface of 14 cartilages. Pathologic examination showed that these findings corresponded to cleft, detachment, erosion, and degeneration. Irregularities in the hyperechoic line at the bone-cartilage interface were revealed by sonography in eight cartilages and were related to irregularity or loss of tidemark, downward displacement of the cartilage, and subchondral callus formation. Dot lesions, corresponding to cleft and degeneration, were noted within one cartilage. Cartilage thickness measured on specimen and by sonography showed no significant difference (p=0.446). Specimen sonography suggested that articular cartilage underwent degenerative histopathological change. Cartilage thickness measured by sonography exactly reflected real thickness

  3. Expression of Transient Receptor Potential Vanilloid (TRPV Channels in Different Passages of Articular Chondrocytes

    Directory of Open Access Journals (Sweden)

    Richard Barrett-Jolley

    2012-04-01

    Full Text Available Ion channels play important roles in chondrocyte mechanotransduction. The transient receptor potential vanilloid (TRPV subfamily of ion channels consists of six members. TRPV1-4 are temperature sensitive calcium-permeable, relatively non-selective cation channels whereas TRPV5 and TRPV6 show high selectivity for calcium over other cations. In this study we investigated the effect of time in culture and passage number on the expression of TRPV4, TRPV5 and TRPV6 in articular chondrocytes isolated from equine metacarpophalangeal joints. Polyclonal antibodies raised against TRPV4, TRPV5 and TRPV6 were used to compare the expression of these channels in lysates from first expansion chondrocytes (P0 and cells from passages 1–3 (P1, P2 and P3 by western blotting. TRPV4, TRPV5 and TRPV6 were expressed in all passages examined. Immunohistochemistry and immunofluorescence confirmed the presence of these channels in sections of formalin fixed articular cartilage and monolayer cultures of methanol fixed P2 chondrocytes. TRPV5 and TRPV6 were upregulated with time and passage in culture suggesting that a shift in the phenotype of the cells in monolayer culture alters the expression of these channels. In conclusion, several TRPV channels are likely to be involved in calcium signaling and homeostasis in chondrocytes.

  4. Development of hybrid scaffolds using ceramic and hydrogel for articular cartilage tissue regeneration.

    Science.gov (United States)

    Seol, Young-Joon; Park, Ju Young; Jeong, Wonju; Kim, Tae-Ho; Kim, Shin-Yoon; Cho, Dong-Woo

    2015-04-01

    The regeneration of articular cartilage consisting of hyaline cartilage and hydrogel scaffolds has been generally used in tissue engineering. However, success in in vivo studies has been rarely reported. The hydrogel scaffolds implanted into articular cartilage defects are mechanically unstable and it is difficult for them to integrate with the surrounding native cartilage tissue. Therefore, it is needed to regenerate cartilage and bone tissue simultaneously. We developed hybrid scaffolds with hydrogel scaffolds for cartilage tissue and with ceramic scaffolds for bone tissue. For in vivo study, hybrid scaffolds were press-fitted into osteochondral tissue defects in a rabbit knee joints and the cartilage tissue regeneration in blank, hydrogel scaffolds, and hybrid scaffolds was compared. In 12th week after implantation, the histological and immunohistochemical analyses were conducted to evaluate the cartilage tissue regeneration. In the blank and hydrogel scaffold groups, the defects were filled with fibrous tissues and the implanted hydrogel scaffolds could not maintain their initial position; in the hybrid scaffold group, newly generated cartilage tissues were morphologically similar to native cartilage tissues and were smoothly connected to the surrounding native tissues. This study demonstrates hybrid scaffolds containing hydrogel and ceramic scaffolds can provide mechanical stability to hydrogel scaffolds and enhance cartilage tissue regeneration at the defect site.

  5. Mechanical vibrations increase the proliferation of articular chondrocytes in high-density culture.

    Science.gov (United States)

    Kaupp, J A; Waldman, S D

    2008-07-01

    Tissue engineering is a promising approach for articular cartilage repair; however, it still has proven a challenge to produce tissue from the limited number of cells that can be extracted from a single individual. Relatively few cell expansion methods exist without the problems of dedifferentiation and/or loss of potency. Previously, it has been shown that mechanical vibrations can enhance chondrocyte proliferation in monolayer culture. Thus, it was hypothesized that chondrocytes grown in high-density culture would respond in a similar fashion while maintaining phenotypic stability. Isolated bovine articular chondrocytes were seeded in high-density culture on Millicell filters and subjected to mechanical vibrations 48 h after seeding. Mechanical vibrations enhanced chondrocyte proliferation at frequencies above 350 Hz, with the peak response occurring at a 1g amplitude for a duration of 30 min. Under these conditions, the gene expression of cartilage-specific and dedifferentiation markers (collagen II, collagen I, and aggrecan) were unchanged by the imposed stimulus. To determine the effect of accumulated extracellular matrix (ECM) on this proliferative response, selected cultures were stimulated under the same conditions after varying lengths of preculture. The amount of accumulated ECM (collagen and proteoglycans) decreased this proliferative response, with the cultures becoming insensitive to the stimulus after 1 week of preculture. Thus, mechanical vibration can serve as an effective means preferentially to stimulate the proliferation of chondrocytes during culture, but its effects appear to be limited to the early stages where ECM accumulation is at a minimum.

  6. Effects of friction on the unconfined compressive response of articular cartilage: a finite element analysis.

    Science.gov (United States)

    Spilker, R L; Suh, J K; Mow, V C

    1990-05-01

    A finite element analysis is used to study a previously unresolved issue of the effects of platen-specimen friction on the response of the unconfined compression test; effects of platen permeability are also determined. The finite element formulation is based on the linear KLM biphasic model for articular cartilage and other hydrated soft tissues. A Galerkin weighted residual method is applied to both the solid phase and the fluid phase, and the continuity equation for the intrinsically incompressible binary mixture is introduced via a penalty method. The solid phase displacements and fluid phase velocities are interpolated for each element in terms of unknown nodal values, producing a system of first order differential equations which are solved using a standard numerical finite difference technique. An axisymmetric element of quadrilateral cross-section is developed and applied to the mechanical test problem of a cylindrical specimen of soft tissue in unconfined compression. These studies show that interfacial friction plays a major role in the unconfined compression response of articular cartilage specimens with small thickness to diameter ratios. PMID:2345443

  7. Infecton is not specific for bacterial osteo-articular infective pathology

    Energy Technology Data Exchange (ETDEWEB)

    Dumarey, Nicolas; Blocklet, Didier; Schoutens, Andre [Division of Nuclear Medicine, Universite Libre de Bruxelles, Brussels (Belgium); Appelboom, Thierry; Tant, Laure [Division of Rheumatology, Universite Libre de Bruxelles, Brussels (Belgium)

    2002-04-01

    The aim of this study was to re-examine, by retrospective analysis of our case material, the specificity and sensitivity of technetium-99m ciprofloxacin scan in discriminating between infection and other conditions. {sup 99m}Tc-ciprofloxacin scintigraphy was performed in 71 patients: 30 patients referred for suspicion of osteomyelitis (OM) or septic arthritis (SA) (group 1) and 41 controls (group 2). Imaging was performed at 4 h post injection and, when possible, at 8 or 24 h post injection. Tracer uptake was visually assessed in different joint groups, and in the sites suspicious for infection. Several soft tissue sites were also evaluated. In the group referred for osteo-articular infection, we found a lower specificity (54.5%) than has previously been reported in the literature. Evaluation of tracer uptake at late imaging did not improve discrimination between sterile and non-sterile inflammation. Additionally, articular uptake was seen in many control patients. Infecton uptake in growth cartilage, thyroid gland, vascular pool, lungs, liver and intestines is discussed. (orig.)

  8. Mesenchymal stem cells as a potent cell source for articular cartilage regeneration

    Institute of Scientific and Technical Information of China (English)

    Mohamadreza; Baghaban; Eslaminejad; Elham; Malakooty; Poor

    2014-01-01

    Since articular cartilage possesses only a weak capac-ity for repair, its regeneration potential is considered one of the most important challenges for orthopedic surgeons. The treatment options, such as marrow stimulation techniques, fail to induce a repair tissue with the same functional and mechanical properties of native hyaline cartilage. Osteochondral transplantation is considered an effective treatment option but is as-sociated with some disadvantages, including donor-site morbidity, tissue supply limitation, unsuitable mechani-cal properties and thickness of the obtained tissue. Although autologous chondrocyte implantation results in reasonable repair, it requires a two-step surgical pro-cedure. Moreover, chondrocytes expanded in culture gradually undergo dedifferentiation, so lose morpho-logical features and specialized functions. In the search for alternative cells, scientists have found mesenchymal stem cells(MSCs) to be an appropriate cellular mate-rial for articular cartilage repair. These cells were origi-nally isolated from bone marrow samples and further investigations have revealed the presence of the cells in many other tissues. Furthermore, chondrogenic dif-ferentiation is an inherent property of MSCs noticedat the time of the cell discovery. MSCs are known to exhibit homing potential to the damaged site at which they differentiate into the tissue cells or secrete a wide spectrum of bioactive factors with regenerative proper-ties. Moreover, these cells possess a considerable im-munomodulatory potential that make them the general donor for therapeutic applications. All of these topics will be discussed in this review.

  9. 3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation.

    Science.gov (United States)

    Smeriglio, Piera; Lai, Janice H; Yang, Fan; Bhutani, Nidhi

    2015-01-01

    Human articular cartilage is highly susceptible to damage and has limited self-repair and regeneration potential. Cell-based strategies to engineer cartilage tissue offer a promising solution to repair articular cartilage. To select the optimal cell source for tissue repair, it is important to develop an appropriate culture platform to systematically examine the biological and biomechanical differences in the tissue-engineered cartilage by different cell sources. Here we applied a three-dimensional (3D) biomimetic hydrogel culture platform to systematically examine cartilage regeneration potential of juvenile, adult, and osteoarthritic (OA) chondrocytes. The 3D biomimetic hydrogel consisted of synthetic component poly(ethylene glycol) and bioactive component chondroitin sulfate, which provides a physiologically relevant microenvironment for in vitro culture of chondrocytes. In addition, the scaffold may be potentially used for cell delivery for cartilage repair in vivo. Cartilage tissue engineered in the scaffold can be evaluated using quantitative gene expression, immunofluorescence staining, biochemical assays, and mechanical testing. Utilizing these outcomes, we were able to characterize the differential regenerative potential of chondrocytes of varying age, both at the gene expression level and in the biochemical and biomechanical properties of the engineered cartilage tissue. The 3D culture model could be applied to investigate the molecular and functional differences among chondrocytes and progenitor cells from different stages of normal or aberrant development. PMID:26484414

  10. Intra-articular use of hyaluronic acid in the treatment of osteoarthritis

    Directory of Open Access Journals (Sweden)

    Alberto Migliore

    2008-06-01

    Full Text Available Alberto Migliore1,2, Mauro Granata31UOS of Rheumatology S. Pietro Fatebenefratelli Hospital, Rome, Italy; 2AFaR Research Center, S. Pietro, Rome, Italy; 3UOS of Rheumatology S. Filippo Neri Hospital, Rome, ItalyAbstract: Osteoarthritis is one of the leading causes of disability in the elderly. The changes in the lubricating properties of synovial fluid lead to significant pain and loss of function. More than ten years have passed from the first studies. Up till now many authors have supported intra-articular hyaluronan (HA therapy as not only a symptom-modifying therapy but also a treatment which may significantly decrease the rate of deterioration of joint structure. In this review we report data relative to knee and hip treatment. The ongoing studies continue to further our understanding of the fundamental mechanisms that likely underlie the therapeutic benefits of this treatment but, despite recent progress, many unresolved issues require further study. Large scale double blind controlled studies must be carried out to confirm these promising data and produce meaningful guidelines.Keywords: osteoarthritis, hyaluronan, intra-articular injections, ultrasound-guided, viscosupplementation

  11. Ongoing studies of cell-based therapies for articular cartilage defects in Japan

    Directory of Open Access Journals (Sweden)

    Ogura T

    2014-12-01

    Full Text Available Takahiro Ogura,1 Akihiro Tsuchiya,2 Shuichi Mizuno1 1Department of Orthopedic Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA; 2Funabashi Orthopaedic Hospital Sports Medicine Center, Funabashi, Chiba, Japan Abstract: Recently, cell-based therapies have generated great interest in the repair of articular cartilage defects and degeneration. Surgical treatments for these indications have multiple options, including marrow stimulation, osteochondral autograft transplant, and autologous chondrocyte implantation. The autologous chondrocyte implantation technique has been improved using a cell scaffold and other devices. Meanwhile, advanced cell-based therapies, including cultured stem cell treatment, have been studied in clinical trials. Most studies have been designed and authorized by institutional review boards and/or the regulatory agencies of the investigators’ countries. For cellular products in regenerative medicine, regulations of many countries are amenable to expedited approval. This paper aims to provide an update on ongoing and prospective cell-based therapies, focusing on articular cartilage injury at designated institutions authorized by the Japanese Pharmaceutical and Medical Device Agency. Keywords: autologous chondrocyte implantation, mesenchymal stem cell, knee joint

  12. Hypoxia Potentiates Anabolic Effects of Exogenous Hyaluronic Acid in Rat Articular Cartilage.

    Science.gov (United States)

    Ichimaru, Shohei; Nakagawa, Shuji; Arai, Yuji; Kishida, Tsunao; Shin-Ya, Masaharu; Honjo, Kuniaki; Tsuchida, Shinji; Inoue, Hiroaki; Fujiwara, Hiroyoshi; Shimomura, Seiji; Mazda, Osam; Kubo, Toshikazu

    2016-01-01

    Hyaluronic acid (HA) is used clinically to treat osteoarthritis (OA), but its pharmacological effects under hypoxic conditions remain unclear. Articular chondrocytes in patients with OA are exposed to a hypoxic environment. This study investigated whether hypoxia could potentiate the anabolic effects of exogenous HA in rat articular cartilage and whether these mechanisms involved HA receptors. HA under hypoxic conditions significantly enhanced the expression of extracellular matrix genes and proteins in explant culture, as shown by real-time reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and dimethylmethylene blue (DMMB) assays. Staining with Safranin-O and immunohistochemical staining with antibody to type II collagen were also enhanced in pellet culture. The expression of CD44 was increased by hypoxia and significantly suppressed by transfection with siRNAs targeting hypoxia-inducible factor 1 alpha (siHIF-1α). These findings indicate that hypoxia potentiates the anabolic effects of exogenous HA by a mechanism in which HIF-1α positively regulates the expression of CD44, enhancing the binding affinity for exogenous HA. The anabolic effects of exogenous HA may increase as OA progresses.

  13. Study on the Microstructure of Human Articular Cartilage/Bone Interface

    Institute of Scientific and Technical Information of China (English)

    Yaxiong Liu; Qin Lian; Jiankang He; Jinna Zhao; Zhongmin Jin; Dichen Li

    2011-01-01

    For improving the theory of gradient microstructure of cartilage/bone interface, human distal femurs were studied. Scanning Electron Microscope (SEM), histological sections and MicroCT were used to observe, measure and model the microstructure of cartilage/bone interface. The results showed that the cartilage/bone interface is in a hierarchical structure which is composed of four different tissue layers. The interlocking of hyaline cartilage and calcified cartilage and that of calcified cartilage and subchondral bone are in the manner of"protrusion-pore" with average diameter of 17.0 μm and 34.1 μm respectively. In addition, the cancellous bone under the cartilage is also formed by four layer hierarchical structure, and the adjacent layers are connected by bone trabecula in the shape of H, I and Y, forming a complex interwoven network structure. Finally, the simplified structure model of the cartilage/bone interface was proposed according to the natural articular cartilage/bone interface. The simplified model is a 4-layer gradient biomimetic structure, which corresponds to four different tissues of natural cartilage/bone interface. The results of this work would be beneficial to the design of bionic scaffold for the tissue engineering of articular cartilage/bone.

  14. Synergy between Piezo1 and Piezo2 channels confers high-strain mechanosensitivity to articular cartilage

    Science.gov (United States)

    Lee, Whasil; Leddy, Holly A.; Chen, Yong; Lee, Suk Hee; Zelenski, Nicole A.; McNulty, Amy L.; Wu, Jason; Beicker, Kellie N.; Coles, Jeffrey; Zauscher, Stefan; Grandl, Jörg; Sachs, Frederick; Liedtke, Wolfgang B.

    2014-01-01

    Diarthrodial joints are essential for load bearing and locomotion. Physiologically, articular cartilage sustains millions of cycles of mechanical loading. Chondrocytes, the cells in cartilage, regulate their metabolic activities in response to mechanical loading. Pathological mechanical stress can lead to maladaptive cellular responses and subsequent cartilage degeneration. We sought to deconstruct chondrocyte mechanotransduction by identifying mechanosensitive ion channels functioning at injurious levels of strain. We detected robust expression of the recently identified mechanosensitive channels, PIEZO1 and PIEZO2. Combined directed expression of Piezo1 and -2 sustained potentiated mechanically induced Ca2+ signals and electrical currents compared with single-Piezo expression. In primary articular chondrocytes, mechanically evoked Ca2+ transients produced by atomic force microscopy were inhibited by GsMTx4, a PIEZO-blocking peptide, and by Piezo1- or Piezo2-specific siRNA. We complemented the cellular approach with an explant-cartilage injury model. GsMTx4 reduced chondrocyte death after mechanical injury, suggesting a possible therapy for reducing cartilage injury and posttraumatic osteoarthritis by attenuating Piezo-mediated cartilage mechanotransduction of injurious strains. PMID:25385580

  15. Induction of vascular endothelial growth factor by nitric oxide in cultured human articular chondrocytes.

    Science.gov (United States)

    Turpaev, K; Litvinov, D; Dubovaya, V; Panasyuk, A; Ivanov, D; Prassolov, V

    2001-06-01

    We investigated the role of nitric oxide (NO) in the control of vascular endothelial growth factor A (VEGF) gene expression in cultured human articular chondrocytes. Cell treatment with the NO-generating compound nitrosoglutathione (GSNO) caused a significant accumulation of 4.4 kb VEGF mRNA, a major VEGF mRNA isoform expressing in chondrocytes. This is the first demonstration that NO can induce VEGF mRNA expression in chondrocytes. VEGF mRNA level was not affected in cells exposed to dibutyryl cGMP, a non-hydrolyzable analog of cGMP, suggesting that the cGMP system is not involved in NO-dependent transcriptional activation of VEGF gene. The GSNO-stimulated induction of VEGF mRNA was slightly attenuated by MAP protein kinase inhibitors PD98058 and SB203580, but was completely blocked in cells incubated with GSNO in the presence of catalase and superoxide dismutase, enzymes scavenging reactive oxygen species (ROS), or in the presence of thiol-containing antioxidants, N-acetyl cysteine and reduced glutathione. These results suggest that in articular chondrocytes the GSNO-induced VEGF gene transcriptional activation is dependent on endogenous ROS production and oxidative thiol modifications.

  16. Enhanced regulatory gene expressions in the blood and articular cartilage of patients with rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Elena Vasilyevna Chetina

    2012-01-01

    Full Text Available Objective: to study the expression ratio of the non-tissue specific regulatory genes mTOR, р21, ATG1, caspase 3, tumor necrosis factor-а (TNF-а, and interleukin-6 (IL-6, as well as matrix metalloproteinase 13 (MMP-13 and X type collagen (COL10A1, cartilage resorption-associated MMP13 and COL10A1 in the blood and knee articular cartilage in patients with rheumatoid arthritis (RA. Subjects and methods. Twenty-five specimens of the distal femoral articular cartilage condyles were studied in 15 RA patients (mean age 52.4+9.1 years after endoprosthetic knee joint replacement and in 10 healthy individuals (mean age 36.0+9.1 years included into the control group. Twenty-eight blood samples taken from 28 RA patients (aged 52+7.6 years prior to endoprosthetic knee joint replacement and 27 blood samples from healthy individuals (mean age 53.6+8.3 years; a control group were also analyzed. Real-time quantitative polymerase chain reaction was applied to estimate the expression of the mTOR, p21, ATG1, caspase 3, TNF-а, IL- 6, COL0A1, and MMP-13 genes. The levels of a protein equivalent in the p70-S6K(activated by mTOR, p21, and caspase 3 genes concerned was measured in the isolated lymphocyte lysates, by applying the commercially available ELISA kits. Total protein in the cell extracts was determined using the Bradford assay procedure. Results. The cartilage samples from patients with end-stage RA exhibited a significantly higher mTOR, ATG1, p21, TNFа, MMP-13, and COL10A1 gene expressions than did those from the healthy individuals. At the same time, IL6 gene expression was much lower than that in the control group. The expressions of the mTOR, ATG1, p21, TNFа, and IL 6 genes in the blood of RA patients were much greater than those in the donors. Caspase 3 expression did not differ essentially in the bloods of the patients with RA and healthy individuals. The bloods failed to show MMP-13 and COL10A1 expressions. High mTOR and p21 gene expressions were

  17. What lies beneath: sub-articular long bone shape scaling in eutherian mammals and saurischian dinosaurs suggests different locomotor adaptations for gigantism.

    Science.gov (United States)

    Bonnan, Matthew F; Wilhite, D Ray; Masters, Simon L; Yates, Adam M; Gardner, Christine K; Aguiar, Adam

    2013-01-01

    Eutherian mammals and saurischian dinosaurs both evolved lineages of huge terrestrial herbivores. Although significantly more saurischian dinosaurs were giants than eutherians, the long bones of both taxa scale similarly and suggest that locomotion was dynamically similar. However, articular cartilage is thin in eutherian mammals but thick in saurischian dinosaurs, differences that could have contributed to, or limited, how frequently gigantism evolved. Therefore, we tested the hypothesis that sub-articular bone, which supports the articular cartilage, changes shape in different ways between terrestrial mammals and dinosaurs with increasing size. Our sample consisted of giant mammal and reptile taxa (i.e., elephants, rhinos, sauropods) plus erect and non-erect outgroups with thin and thick articular cartilage. Our results show that eutherian mammal sub-articular shape becomes narrow with well-defined surface features as size increases. In contrast, this region in saurischian dinosaurs expands and remains gently convex with increasing size. Similar trends were observed in non-erect outgroup taxa (monotremes, alligators), showing that the trends we report are posture-independent. These differences support our hypothesis that sub-articular shape scales differently between eutherian mammals and saurischian dinosaurs. Our results show that articular cartilage thickness and sub-articular shape are correlated. In mammals, joints become ever more congruent and thinner with increasing size, whereas archosaur joints remained both congruent and thick, especially in sauropods. We suggest that gigantism occurs less frequently in mammals, in part, because joints composed of thin articular cartilage can only become so congruent before stress cannot be effectively alleviated. In contrast, frequent gigantism in saurischian dinosaurs may be explained, in part, by joints with thick articular cartilage that can deform across large areas with increasing load.

  18. What lies beneath: sub-articular long bone shape scaling in eutherian mammals and saurischian dinosaurs suggests different locomotor adaptations for gigantism.

    Directory of Open Access Journals (Sweden)

    Matthew F Bonnan

    Full Text Available Eutherian mammals and saurischian dinosaurs both evolved lineages of huge terrestrial herbivores. Although significantly more saurischian dinosaurs were giants than eutherians, the long bones of both taxa scale similarly and suggest that locomotion was dynamically similar. However, articular cartilage is thin in eutherian mammals but thick in saurischian dinosaurs, differences that could have contributed to, or limited, how frequently gigantism evolved. Therefore, we tested the hypothesis that sub-articular bone, which supports the articular cartilage, changes shape in different ways between terrestrial mammals and dinosaurs with increasing size. Our sample consisted of giant mammal and reptile taxa (i.e., elephants, rhinos, sauropods plus erect and non-erect outgroups with thin and thick articular cartilage. Our results show that eutherian mammal sub-articular shape becomes narrow with well-defined surface features as size increases. In contrast, this region in saurischian dinosaurs expands and remains gently convex with increasing size. Similar trends were observed in non-erect outgroup taxa (monotremes, alligators, showing that the trends we report are posture-independent. These differences support our hypothesis that sub-articular shape scales differently between eutherian mammals and saurischian dinosaurs. Our results show that articular cartilage thickness and sub-articular shape are correlated. In mammals, joints become ever more congruent and thinner with increasing size, whereas archosaur joints remained both congruent and thick, especially in sauropods. We suggest that gigantism occurs less frequently in mammals, in part, because joints composed of thin articular cartilage can only become so congruent before stress cannot be effectively alleviated. In contrast, frequent gigantism in saurischian dinosaurs may be explained, in part, by joints with thick articular cartilage that can deform across large areas with increasing load.

  19. What lies beneath: sub-articular long bone shape scaling in eutherian mammals and saurischian dinosaurs suggests different locomotor adaptations for gigantism.

    Science.gov (United States)

    Bonnan, Matthew F; Wilhite, D Ray; Masters, Simon L; Yates, Adam M; Gardner, Christine K; Aguiar, Adam

    2013-01-01

    Eutherian mammals and saurischian dinosaurs both evolved lineages of huge terrestrial herbivores. Although significantly more saurischian dinosaurs were giants than eutherians, the long bones of both taxa scale similarly and suggest that locomotion was dynamically similar. However, articular cartilage is thin in eutherian mammals but thick in saurischian dinosaurs, differences that could have contributed to, or limited, how frequently gigantism evolved. Therefore, we tested the hypothesis that sub-articular bone, which supports the articular cartilage, changes shape in different ways between terrestrial mammals and dinosaurs with increasing size. Our sample consisted of giant mammal and reptile taxa (i.e., elephants, rhinos, sauropods) plus erect and non-erect outgroups with thin and thick articular cartilage. Our results show that eutherian mammal sub-articular shape becomes narrow with well-defined surface features as size increases. In contrast, this region in saurischian dinosaurs expands and remains gently convex with increasing size. Similar trends were observed in non-erect outgroup taxa (monotremes, alligators), showing that the trends we report are posture-independent. These differences support our hypothesis that sub-articular shape scales differently between eutherian mammals and saurischian dinosaurs. Our results show that articular cartilage thickness and sub-articular shape are correlated. In mammals, joints become ever more congruent and thinner with increasing size, whereas archosaur joints remained both congruent and thick, especially in sauropods. We suggest that gigantism occurs less frequently in mammals, in part, because joints composed of thin articular cartilage can only become so congruent before stress cannot be effectively alleviated. In contrast, frequent gigantism in saurischian dinosaurs may be explained, in part, by joints with thick articular cartilage that can deform across large areas with increasing load. PMID:24130690

  20. Analgesia intra-articular com morfina, bupivacaína ou fentanil após operação de joelho por videoartroscopia Analgesia intra-articular con morfina, bupivacaína o fentanil después de operación de rodilla por videoartroscopia Intra-articular analgesia with morphine, bupivacaine or fentanyl after knee video-arthroscopy surgery

    Directory of Open Access Journals (Sweden)

    Rogério Helcias de Souza

    2002-09-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O uso de métodos que promovam analgesia para dor do joelho sem prejudicar a função motora tem sido bastante pesquisado. O objetivo do presente estudo foi comparar o efeito analgésico da morfina, da bupivacaína e do fentanil, com a solução fisiológica, injetada por via intra-articular após operação de joelho por videoartroscopia. MÉTODO: Sessenta pacientes foram divididos de forma aleatória, em quatro grupos: GI (n=15 - 10 ml de solução fisiológica; GII (n = 15 - 2 mg de morfina diluídos para 10 ml de solução fisiológica; GIII (n = 15 - 10 ml de bupivacaína a 0,25%; GIV (n = 15 - 100 µg de fentanil diluídos para 10 ml de solução fisiológica, injetados ao término da operação. Todos os pacientes foram submetidos à anestesia subaracnóidea com 15 mg de bupivacaína hiperbárica. A intensidade da dor foi avaliada pela escala analógica visual (imediatamente após o término da operação e após 6, 12, 18 e 24 horas, bem como a necessidade de complementação analgésica (dipirona 1 g por via venosa. Foram anotados os possíveis efeitos colaterais. RESULTADOS: Não houve diferença significativa na intensidade da dor entre os grupos, na quase totalidade dos tempos estudados. Houve diferença estatística até seis horas, quando o grupo fentanil apresentou intensidade da dor significativamente menor. O grupo morfina necessitou de maior número de complementações com dipirona. Os efeitos colaterais foram mínimos, sem significância estatística. CONCLUSÕES: Não houve diferença significativa entre a analgesia promovida pelas soluções estudadas na maioria dos tempos investigados.JUSTIFICATIVA Y OBJETIVOS: El uso de métodos que promuevan analgesia para el dolor de rodilla sin perjudicar la función motora ha sido bastante pesquisado. El objetivo del presente estudio fue comparar el efecto analgésico de la morfina, de la bupivacaína y del fentanil, con la solución fisiológica, inyectada

  1. Results of a survey about specific inflammatory bowel disease units in Andalusia Resultados de la encuesta sobre consulta monográfica de enfermedad inflamatoria intestinal realizada en Andalucía

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    J. M. Herrerías Gutiérrez

    2009-03-01

    Full Text Available Background: inflammatory bowel disease (IBD has become one of the most prevalent pathologies in Gastroenterology Units, which added to its clinical outcome, treatment, the complexity of affected patients and the need to be continuously updated for the correct management of the disease, have made essential the presence of specific IBD units in each hospital. Objectives: the primary aims of this study were: a to reveal the existence of these units in our area and how they work; and b to draw conclusions regarding the necessary resources in these units and their aims. Material and methods: In order to analyse the presence of these specialized units and the available resources in Andalusian hospitals, a 24-question survey was designed, being answered by 11 hospitals. The evaluated questions included the number of days patients are attended and the number of physicians attending the unit, the number of available healthcare assistants, if emergencies are attended or not, if there is an activated telephone number for patient consultation, if a day care unit is available and if new treatments are easily accessible. Results: a specific IBD unit is present in all studied hospitals attending more than 11 patients each, although in the 63.4% of the cases patients are not attended more than 3 days per week. On the other hand, the 81.8% of the included hospitals attend emergencies although only the 54.5% of them had a specific telephone number for patient attendance. Conclusions: a specific IBD unit is present in many Andalusian hospitals, although some deficiencies can be observed. The general opinion of this Group is that these units are necessary in order to properly attend, monitorize and treat patients affected by IBD.Introducción: la enfermedad inflamatoria intestinal (EII se está convirtiendo en una de las patologías más prevalentes en las consultas de digestivo. Esta mayor prevalencia de la enfermedad, su evolución, tratamiento y el tipo de

  2. Joint position statement by "Sociedad Española de Patología Digestiva" (Spanish Society of Gastroenterology and "Sociedad Española de Farmacología" (Spanish Society of Pharmacology on biosimilar therapy for inflammatory bowel disease Posición conjunta de la Sociedad Española de Patología Digestiva y de la Sociedad Española de Farmacología sobre el tratamiento con biosimilares en la enfermedad inflamatoria intestinal

    Directory of Open Access Journals (Sweden)

    Federico Argüelles-Arias

    2013-01-01

    ógicos, fabricados mediante, o a partir de organismos vivos utilizando biotecnología, han supuesto una revolución terapéutica en el control de la enfermedad inflamatoria intestinal (EII. Al presente, en esta indicación y en nuestro país, solo se dispone de dos biológicos autorizados, infliximab (IFX y adalimumab (ADA, ambos anticuerpos monoclonales frente al factor de necrosis tumoral alfa. La evidencia de eficacia con ambos tratamientos es sólida con niveles de evidencia científica máxima. La proximidad de la expiración de las patentes de estos dos biológicos ha abierto la posibilidad de entrada en el mercado de los denominados biosimilares en la indicación de tratamiento de la EII. Aunque conceptualmente los biosimilares son para los medicamentos biológicos lo que los genéricos para los químicos, la complejidad estructural de los biosimilares, así como su propia variabilidad biológica y la de su producción, obligan a considerar como muy diferentes los procesos de validación de su uso en humanos respecto de los mencionados genéricos. Así, en nuestro medio, y contando con la garantía de la Agencia Española del Medicamento y Productos Sanitarios (AEMPS, son de aplicación las reglas del juego dictadas por la "European Medicines Agency" (EMA que ha establecido una serie de etapas o escalones a superar antes de poder obtener la aprobación para un biosimilar. No obstante, a pesar de la existencia de estas recomendaciones de la EMA, que deben cumplirse para que un biosimilar sea aprobado en nuestro mercado, persisten algunas incertidumbres relevantes que solo las futuras decisiones de la EMA y de la AEMPS pueden aclarar. La posición del presente grupo de trabajo es que el desarrollo de un biosimilar debe hacerse en el contexto de las normas establecidas certificando de esta manera su eficacia y seguridad. Igualmente este grupo de trabajo considera que no deben extrapolarse los resultados obtenidos en los estudios realizados en artritis reumatoide (AR a la EII, en

  3. Legionella pneumophila Arthritis: use of medium specific for Mycobacteria for isolation of L. pneumophila in culture of articular fluid specimens.

    Science.gov (United States)

    Bemer, Pascale; Leautez, Sophie; Ninin, Emmanuelle; Jarraud, Sophie; Raffi, François; Drugeon, Henri

    2002-07-01

    We report the first case, to our knowledge, of acute purulent arthritis due to Legionella pneumophila in an immunosuppressed patient. L. pneumophila was isolated from samples of blood and articular fluid cultured with use of medium specific for mycobacteria (Bactec 13A medium).

  4. Clinical Trial and In Vitro Study for the Role of Cartilage and Synovia in Acute Articular Infection

    DEFF Research Database (Denmark)

    Langenmair, E. R.; Kubosch, E. J.; Salzmann, G. M.;

    2015-01-01

    Objective. Osteoarthritis is a long-term complication of acute articular infections. However, the roles of cartilage and synovia in this process are not yet fully understood. Methods. Patients with acute joint infections were enrolled in a prospective clinical trial and the cytokine composition o...

  5. PKCa Agonists Enhance the Protective Effect of Hyaluronic Acid on Nitric Oxide-Induced Apoptosis of Articular Chondrocytes in Vitro

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    Jian-lin Zhou

    2013-12-01

    The results may be showed that PKCa regulate the expresion of caspase-3, which contribute to the apoptosis of chondrocytes induced by NO. PKC α agonists enhance the protective effect of hyaluronic acid on nitric oxide-induced articular chondrocytes apoptosis.

  6. Quantitative description of collagen structure in the articular cartilage of the young and adult equine distal metacarpus

    NARCIS (Netherlands)

    Turnhout, van M.C.; Haazelager, M.B.; Gijsen, M.A.L.; Schipper, H.; Kranenbarg, S.; Leeuwen, van J.L.

    2008-01-01

    The orientation and organisation of collagen fibrils play an important role in the mechanical functioning of the articular cartilage (AC) that covers the surfaces in the diarthrodial joints. In the adult animal, typically an arcade like 'Benninghoff structure' is found. Because the remodelling capac

  7. External Fixation versus two-stage Open Reduction Internal Fixation of distal intra-articular Tibia fractures; a Systematic Review

    DEFF Research Database (Denmark)

    Ladeby Erichsen, Julie; Jensen, Carsten; Damborg, Frank Lindhøj;

    Background: Distal Intra-Articular Tibia Fractures (DIATF) is challenging to treat and severe loss of physical function affecting working abilities has been reported. Aim: To investigate differences in physical function and complications following DIATF surgery with two-stage Open Reduction...

  8. Intracapsular and para- articular chondroma of knee. A report of four cases and review of the literature

    International Nuclear Information System (INIS)

    Background. Intracapsular and para-articular chondroma is a rare variant of the extraskeletal chondromas. It arises from the capsule and/or the para-articular connective tissue of the large joints (mostly the knee) and is a result of cartilaginous metaplasia. In course of time these tumors ossify and this is where their second name comes from: Para-articular osteochondromas. According to Jaffe, not dependent on the degree of ossification of this tumor, there is one single entity in question. Cases report. We report four new cases of para-articular chondroma of the knee. On physical examination there was slow-growing solid mass in the knee and moderate pain, the radiological findings and CT scan show soft-tissue mass with variable amount of ossification, and on histological examination the presence of mature hyaline and connective cartilage was confirmed in all of the cases. Conclusions. The diagnosis of these benign tumors is made with correlation of clinical, radiological and histological features. The treatment of choice is surgical excision in toto. (author)

  9. The relationship between ultra-short telomeres, aging of articular cartilage and the development of human hip osteoarthritis

    DEFF Research Database (Denmark)

    Harbo, M; Delaisse, J M; Kjaersgaard-Andersen, P;

    2013-01-01

    Ultra-short telomeres caused by stress-induced telomere shortening are suggested to induce chondrocyte senescence in human osteoarthritic knees. Here we have further investigated the role of ultra-short telomeres in the development of osteoarthritis (OA) and in aging of articular cartilage in human...

  10. Pain and disability in patients with osteoarthritis of hip or knee: the relationship with articular, kinesiological, and psychological characteristics.

    NARCIS (Netherlands)

    Baar, M.E. van; Dekkers, J.; Lemmens, J.A.M.; Oostendorp, R.A.B.; Bijlsma, J.W.J.

    1998-01-01

    Objective: To determine to what extent articular, kinesiological, and psychological factors each contribute to pain and disability in patients with osteoarthritis (OA), after controlling for other factors. Methods: Cross sectional study among 200 patients with OA of the hip or knee. Dependent variab

  11. Electrostatic and Non-Electrostatic Contributions of Proteoglycans to the Compressive Equilibrium Modulus of Bovine Articular Cartilage

    OpenAIRE

    Guterl, Clare Canal; Hung, Clark T.; Ateshian, Gerard A.

    2010-01-01

    This study presents direct experimental evidence for assessing the electrostatic and nonelectrostatic contributions of proteoglycans to the compressive equilibrium modulus of bovine articular cartilage. Immature and mature bovine cartilage samples were tested in unconfined compression and their depth-dependent equilibrium compressive modulus was determined using strain measurements with digital image correlation analysis. The electrostatic contribution was assessed by testing samples in isoto...

  12. Papel de la proteína quinasa dependiente de cGMP (PKG) y de la c-Src tirosina quinasa en la regulación antagónica del óxido nítrico sobre la apoptosis

    OpenAIRE

    Tejedo Huamán, Juan Rigoberto

    2000-01-01

    En los últimos años se han acumulado evidencias concernientes al papel de la apoptosis en el control de la población de células beta en la patogénesis de la IDDM. Así se ha visto que la apoptosis en células beta de roedores está mediada por la generación de óxido nítrico (NO) en un proceso regulado por citoquinas inflamatorias. Sin embargo respecto al papel del NO en el control de la apoptosis se han encontrado varios mecanismos por los que altas concentraciones de NO la inducen y bajas conce...

  13. Visibilizar, influenciar y modificar: despenalización del aborto en Colombia

    Directory of Open Access Journals (Sweden)

    Claudia Gómez

    2006-04-01

    Full Text Available A través del recuento y el análisis de las estrategias jurídica, de alianzas y de comunicación del proyecto LAICIA(Litigio de alto impacto en Colombia: la inconstitucionalidad del aborto el texto pretende validar las acciones públicas de inconstitucionalidad como una herramienta de cambio social y convocar a la sincronización de agendas para adelantar campañas que promuevan los derechos de las mujeres. El artículo hace especial énfasis en la necesidad de articular diferentes actores a lo largo de todo el curso de acción de las políticas públicas y en particular sobre la estructuración de canales de comunicación.

  14. Manifestações articulares nas viroses exantemáticas Joint complaints in exanthematic diseases

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    Solange Artimos de Oliveira

    1999-04-01

    Full Text Available A freqüência de manifestações articulares foi avaliada em 251 pacientes com diagnóstico clínico e laboratorial (detecção de IgM por ensaio imunoenzimático de virose exantemática. As artropatias (artralgia e/ou artrite foram mais observadas nos casos de dengue (49% e de rubéola (38,2% do que naqueles com parvovirose humana (30% e sarampo (28,1%. Com exceção do sarampo, as artropatias predominaram nos adultos (315 anos de idade, sendo tal diferença estatisticamente significativa. A ocorrência maior de artropatias em adultos foi mais evidente nos pacientes com parvovirose (75%, rubéola (65% e dengue (57,7% do que naqueles com sarampo (31%. As queixas articulares também predominaram nos pacientes do sexo feminino para todas as viroses avaliadas. Os resultados encontrados demonstram o freqüente acometimento articular nas doenças estudadas, e indicam a necessidade de comprovação laboratorial para o diagnóstico diferencial entre elas.The frequency of arthropathy was evaluated in 251 patients with clinical and serological diagnosis (specific IgM detection by enzyme immunoassay of exanthematic disease. Arthropathy (arthralgia and/or arthritis was more frequent in dengue fever (49% and rubella (38.2% cases than in human parvovirus (30% and measles (28.1% cases. Except for measles cases, joint complaints prevailed in adults (315 years of age and this difference was significant. The higher frequency of arthropathy in adults was more evident in human parvovirus (75%, rubella (65% and dengue fever (57.7% cases than in measles cases (31%. Arthropathy was also more frequent in females for all rash diseases studied. The results of this study showed the high occurrence of joint complaints in the diseases described here and the importance of laboratory confirmation for their differential diagnosis.

  15. Vulnerability of the Superficial Zone of Immature Articular Cartilage to Compressive Injury

    Energy Technology Data Exchange (ETDEWEB)

    Rolauffs, R.; Muehleman, C; Li, J; Kurz, B; Kuettner, K; Frank, E; Grodzinsky, A

    2010-01-01

    The zonal composition and functioning of adult articular cartilage causes depth-dependent responses to compressive injury. In immature cartilage, shear and compressive moduli as well as collagen and sulfated glycosaminoglycan (sGAG) content also vary with depth. However, there is little understanding of the depth-dependent damage caused by injury. Since injury to immature knee joints most often causes articular cartilage lesions, this study was undertaken to characterize the zonal dependence of biomechanical, biochemical, and matrix-associated changes caused by compressive injury. Disks from the superficial and deeper zones of bovine calves were biomechanically characterized. Injury to the disks was achieved by applying a final strain of 50% compression at 100%/second, followed by biomechanical recharacterization. Tissue compaction upon injury as well as sGAG density, sGAG loss, and biosynthesis were measured. Collagen fiber orientation and matrix damage were assessed using histology, diffraction-enhanced x-ray imaging, and texture analysis. Injured superficial zone disks showed surface disruption, tissue compaction by 20.3 {+-} 4.3% (mean {+-} SEM), and immediate biomechanical impairment that was revealed by a mean {+-} SEM decrease in dynamic stiffness to 7.1 {+-} 3.3% of the value before injury and equilibrium moduli that were below the level of detection. Tissue areas that appeared intact on histology showed clear textural alterations. Injured deeper zone disks showed collagen crimping but remained undamaged and biomechanically intact. Superficial zone disks did not lose sGAG immediately after injury, but lost 17.8 {+-} 1.4% of sGAG after 48 hours; deeper zone disks lost only 2.8 {+-} 0.3% of sGAG content. Biomechanical impairment was associated primarily with structural damage. The soft superficial zone of immature cartilage is vulnerable to compressive injury, causing superficial matrix disruption, extensive compaction, and textural alteration, which results

  16. Intra-articular methotrexate associated to lipid nanoemulsions: anti-inflammatory effect upon antigen-induced arthritis

    Directory of Open Access Journals (Sweden)

    Mello SB

    2013-02-01

    Full Text Available Suzana BV Mello,1 Elaine R Tavares,2 Adriana Bulgarelli,2 Eloisa Bonfá,1 Raul C Maranhão2,31Rheumatology Division, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; 2Lipid Metabolism Laboratory, the Heart Institute (INCOR of the Medical School Hospital, São Paulo, Brazil; 3Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilObjective: Commercial methotrexate formulations (MTX have poor anti-inflammatory action for intra-articular treatment of rheumatoid arthritis. Our aim was to investigate whether an association between methotrexate and lipidic nanoemulsions (LDE could improve MTX intra-articular action.Methods: For its association to LDE, MTX was previously esterified with dodecyl bromide. LDE-MTX was prepared by high pressure homogenization. Antigen-induced arthritis (AIA was achieved in rabbits sensitized with methylated bovine serum albumin, and the rabbits were subsequently intra-articularly injected with the antigen. Twenty-four hours after AIA induction, groups of four to nine rabbits were intra-articularly injected with increasing doses (0.0625–0.5 µmol/kg of LDE-MTX, and were compared to treatment with 0.5 µmol/kg commercial MTX, LDE alone, and saline (controls. Synovial fluid was collected 48 hours after AIA induction for analysis of protein leakage and cell content. Synovial membranes were collected for histopathology. Uptake of LDE labeled with 3H-cholesteryl ether by the synovial tissue was also determined.Results: Uptake of radioactive LDE by arthritic joints was 2.5-fold greater than by normal joints. Treatment with intra-articular LDE-MTX elicited a clear dose response pattern by reducing the synovial leukocyte infiltrate (P = 0.004 and protein leakage (P = 0.032 when compared with arthritic non-treated joints. In contrast, the intra-articular injection of commercial MTX and LDE did not reduce leukocyte infiltrate or protein leakage. Toxicity to treatment was not observed

  17. Treatment with recombinant lubricin attenuates osteoarthritis by positive feedback loop between articular cartilage and subchondral bone in ovariectomized rats.

    Science.gov (United States)

    Cui, Zhuang; Xu, Changpeng; Li, Xue; Song, Jinqi; Yu, Bin

    2015-05-01

    Osteoarthritis (OA) is a most commonly multifactorial degenerative joint disease along with the aging population, particularly in postmenopausal women. During the onset of OA, articular cartilage and subchondral bone act in concert as a functional unit. This present study is to investigate the effects of early or late treatment with recombinant lubricin on the onset of osteoarthritis (OA) in ovariectomized (OVX) rats. We found that both early and late recombinant lubricin treatments attenuated the onset of OA by positive feedback loop between articular cartilage and subchondral bone, although late treatment contributed to a lesser effect compared with early treatment. Specifically, treatment with recombinant lubricin protected articular cartilage from degeneration, demonstrated by lower proteoglycan loss, lower OARSI scores, less calcification cartilage zone and reduced immunostaining for collagen X (Col X) and matrix metalloproteinase (MMP-13) but increased the expression of lubricin, in comparison with vehicle-treated OVX rat group. Further, chondroprotective effects of lubricin normalized bone remodeling in subchondral bone underneath. It's suggested that treatment with recombinant lubricin inhibited the elevation of TRAP and Osterix positive cells in OVX rats and led to the normalization of subchondral bone microarchitectures with the suppression of subsidence of bone volume ratio (BV/TV) and trabecular thickness (Tb.Th) and the increase of trabecular separation (Tb.Sp) in vehicle-treated OVX rats. What's more, the normalization of subchondral bone in turn attenuated the articular cartilage erosion by inhibiting vascular invasion from subchondral bone to calcified cartilage zone, exemplified by inhibiting the elevation of CD31 positive cells in calcified cartilage and angiography in subchondral bone. Together, these results shed light that both early and late recombinant lubricin treatments attenuate the onset of OA by balancing the interplay between articular

  18. The effects of physical activity on apoptosis and lubricin expression in articular cartilage in rats with glucocorticoid-induced osteoporosis.

    Science.gov (United States)

    Musumeci, Giuseppe; Loreto, Carla; Leonardi, Rosalia; Castorina, Sergio; Giunta, Salvatore; Carnazza, Maria Luisa; Trovato, Francesca Maria; Pichler, Karin; Weinberg, Annelie Martina

    2013-05-01

    Glucocorticoids are considered the most powerful anti-inflammatory and immunomodulating drugs. However, a number of side-effects are well documented in different diseases, including articular cartilage, where increases or decreases in the synthesis of hormone-dependent extracellular matrix components are seen. The objective of this study has been to test the effects of procedures or drugs affecting bone metabolism on articular cartilage in rats with prednisolone-induced osteoporosis and to evaluate the outcomes of physical activity with treadmill and vibration platform training on articular cartilage. The animals were divided into 5 groups, and bone and cartilage evaluations were performed using whole-body scans and histomorphometric analysis. Lubricin and caspase-3 expression were evaluated by immunohistochemistry, Western blot analysis and biochemical analysis. These results confirm the beneficial effect of physical activity on the articular cartilage. The effects of drug therapy with glucocorticoids decrease the expression of lubricin and increase the expression of caspase-3 in the rats, while after physical activity the values return to normal compared to the control group. Our findings suggest that it might be possible that mechanical stimulation in the articular cartilage could induce the expression of lubricin, which is capable of inhibiting caspase-3 activity, preventing chondrocyte death. We can assume that the physiologic balance between lubricin and caspase-3 could maintain the integrity of cartilage. Therefore, in certain diseases such as osteoporosis, mechanical stimulation could be a possible therapeutic treatment. With our results we can propose the hypothesis that physical activity could also be used as a therapeutic treatment for cartilage disease such as osteoarthritis.

  19. Treatment with recombinant lubricin attenuates osteoarthritis by positive feedback loop between articular cartilage and subchondral bone in ovariectomized rats.

    Science.gov (United States)

    Cui, Zhuang; Xu, Changpeng; Li, Xue; Song, Jinqi; Yu, Bin

    2015-05-01

    Osteoarthritis (OA) is a most commonly multifactorial degenerative joint disease along with the aging population, particularly in postmenopausal women. During the onset of OA, articular cartilage and subchondral bone act in concert as a functional unit. This present study is to investigate the effects of early or late treatment with recombinant lubricin on the onset of osteoarthritis (OA) in ovariectomized (OVX) rats. We found that both early and late recombinant lubricin treatments attenuated the onset of OA by positive feedback loop between articular cartilage and subchondral bone, although late treatment contributed to a lesser effect compared with early treatment. Specifically, treatment with recombinant lubricin protected articular cartilage from degeneration, demonstrated by lower proteoglycan loss, lower OARSI scores, less calcification cartilage zone and reduced immunostaining for collagen X (Col X) and matrix metalloproteinase (MMP-13) but increased the expression of lubricin, in comparison with vehicle-treated OVX rat group. Further, chondroprotective effects of lubricin normalized bone remodeling in subchondral bone underneath. It's suggested that treatment with recombinant lubricin inhibited the elevation of TRAP and Osterix positive cells in OVX rats and led to the normalization of subchondral bone microarchitectures with the suppression of subsidence of bone volume ratio (BV/TV) and trabecular thickness (Tb.Th) and the increase of trabecular separation (Tb.Sp) in vehicle-treated OVX rats. What's more, the normalization of subchondral bone in turn attenuated the articular cartilage erosion by inhibiting vascular invasion from subchondral bone to calcified cartilage zone, exemplified by inhibiting the elevation of CD31 positive cells in calcified cartilage and angiography in subchondral bone. Together, these results shed light that both early and late recombinant lubricin treatments attenuate the onset of OA by balancing the interplay between articular

  20. 24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats

    Science.gov (United States)

    Boyan, Barbara D.; Hyzy, Sharon L.; Pan, Qingfen; Scott, Kayla M.; Coutts, Richard D.; Healey, Robert; Schwartz, Zvi

    2016-01-01

    Osteoarthritis (OA) in humans is associated with low circulating 25-hydroxyvitamin D3 [25(OH)D3]. In vitamin D replete rats, radiolabeled 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D3] accumulates in articular cartilage following injection of [3H]-25(OH)D3. Previously, we showed that 24R,25(OH)2D3 blocks chondrocyte apoptosis via phospholipase D and p53, suggesting a role for 24R,25(OH)2D3 in maintaining cartilage health. We examined the ability of 24R,25(OH)2D3 to prevent degenerative changes in articular cartilage in an OA-like environment and the potential mechanisms involved. In vitro, rat articular chondrocytes were treated with IL-1β with and without 24R,25(OH)2D3 or 1α,25(OH)2D3. 24R,25(OH)2D3 but not 1α,25(OH)2D3 blocked the effects of IL-1β in a dose-dependent manner, and its effect was partially mediated through the TGF-β1 signaling pathway. In vivo, unilateral anterior cruciate ligament transections were performed in immunocompetent rats followed by intra-articular injections of 24R,25(OH)2D3 or vehicle (t = 0, 7, 14, 21 days). Tissues were harvested on day 28. Joints treated with vehicle had changes typical of OA whereas joints treated with 24R,25(OH)2D3 had less articular cartilage damage and levels of inflammatory mediators. These results indicate that 24R,25(OH)2D3 protects against OA, and suggest that it may be a therapeutic approach for preventing trauma-induced osteoarthritis. PMID:27575371

  1. Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.

    Science.gov (United States)

    Liao, I-Chien; Moutos, Franklin T; Estes, Bradley T; Zhao, Xuanhe; Guilak, Farshid

    2013-12-17

    The development of synthetic biomaterials that possess mechanical properties that mimic those of native tissues remains an important challenge to the field of materials. In particular, articular cartilage is a complex nonlinear, viscoelastic, and anisotropic material that exhibits a very low coefficient of friction, allowing it to withstand millions of cycles of joint loading over decades of wear. Here we show that a three-dimensionally woven fiber scaffold that is infiltrated with an interpenetrating network hydrogel can provide a functional biomaterial that provides the load-bearing and tribological properties of native cartilage. An interpenetrating dual-network "tough-gel" consisting of alginate and polyacrylamide was infused into a porous three-dimensionally woven poly(ε-caprolactone) fiber scaffold, providing a versatile fiber-reinforced composite structure as a potential acellular or cell-based replacement for cartilage repair. PMID:24578679

  2. Effects of Balsamodendron mukul Gum Resin Extract on Articular Cartilage in Papain-induced Osteoarthritis.

    Science.gov (United States)

    Manjhi, Jayanand; Gupta, Maneesh; Sinha, Anvesha; Rawat, Beena; Rai, Durg V

    2016-07-01

    Context • Osteoarthritis (OA) is one of the most prevalent chronic diseases of the musculoskeleton, causing functional disability among older adults. Management of OA includes conventional pharmacological treatments consisting primarily of nonsteroidal anti-inflammatory drugs (NSAIDs), acetaminophen, physiotherapy, and surgical procedures. The medications are not ideal therapeutic agents; NSAIDs in particular can cause serious side effects. Objective • The study was conducted to investigate the effects of Balsamodendron mukul (BDM) gum resin extract on cartilage damage and microstructural changes in the subchondral bone of rats with papain-induced, osteoarthritic knee joints. Design • The authors designed a parallel randomized, controlled study to examine the effects of 3 concentrations of BDM on OA in a murine model. Setting • The present study was undertaken at the research laboratory, Faculty of Biological Engineering, Shobhit University (Modipuram, Meerut, India). Intervention • OA was induced by intra-articular injections of 0.2 mL of 4% papain solution and 0.1 mL of 0.03 M cysteine through the patellar ligament using a 26-gauge, 1.27-cm needle. The rats in the sham group received same volume of isotonic sodium chloride solution. The rats were divided into 6 groups : (1) control group-fresh rats, with ages and genders similar to those of the other groups but with no induction of OA and no treatments; (2) sham group-rats receiving a sham induction of OA using an intra-articular injection of saline of the same volume as the papain given to all OA rats but no treatments; (3) OA group-rats induced with OA but receiving no treatments; (4) OA + BDM (10%) group-rats induced with OA that received a 10% dose of BDM; (5) OA + BDM (20%) group-rats induced with OA that received a 20% dose of BDM; and (6) OA + BDM (40%) group-rats induced with OA that received a 40% dose of BDM. Rats in the treatment groups were fed their respective doses of BDM extract for 30 d

  3. Biochemical and metabolic abnormalities in normal and osteoarthritic human articular cartilage

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, J.; Treadwell, B.V.; Mankin, H.J.

    1984-01-01

    Incorporation of radioactive precursors into macromolecules was studied with human normal and osteoarthritic articular cartilage organ culture. Analysis of the salt extracted matrix components separated by cesium chloride buoyant density gradient centrifugation showed an increase in the specific activities of all gradient fractions prepared from the osteoarthritic cartilage. Further analysis of these fractions showed the osteoarthritic cartilage contained 5 times as much sulfate incorporated into proteoglycans, and an even greater amount of 3H-glucosamine incorporated into material sedimenting to the middle of the gradient. Greater than half of this radioactive middle fraction appears to be hyaluronate, as judged by the position it elutes from a DEAE column and its susceptibility to hyaluronidase digestion. This study supports earlier findings showing increased rates of macromolecular synthesis in osteoarthritis, and in addition, an even greater synthetic rate for hyaluronic acid is demonstrated.

  4. Nonlinear mechanical response of the extracellular matrix: learning from articular cartilage

    Science.gov (United States)

    Kearns, Sarah; Das, Moumita

    2015-03-01

    We study the mechanical structure-function relations in the extracellular matrix (ECM) with focus on nonlinear shear and compression response. As a model system, our study focuses on the ECM in articular cartilage tissue which has two major mechanobiological components: a network of the biopolymer collagen that acts as a stiff, reinforcing matrix, and a flexible aggrecan network that facilitates deformability. We model this system as a double network hydrogel made of interpenetrating networks of stiff and flexible biopolymers respectively. We study the linear and nonlinear mechanical response of the model ECM to shear and compression forces using a combination of rigidity percolation theory and energy minimization approaches. Our results may provide useful insights into the design principles of the ECM as well as biomimetic hydrogels that are mechanically robust and can, at the same time, easily adapt to cues in their surroundings.

  5. Minimally-invasive treatment of high velocity intra-articular fractures of the distal tibia.

    LENUS (Irish Health Repository)

    Leonard, M

    2012-02-01

    The pilon fracture is a complex injury. The purpose of this study was to evaluate the outcome of minimally invasive techniques in management of these injuries. This was a prospective study of closed AO type C2 and C3 fractures managed by early (<36 hours) minimally invasive surgical intervention and physiotherapist led rehabilitation. Thirty patients with 32 intra-articular distal tibial fractures were treated by the senior surgeon (GK). Our aim was to record the outcome and all complications with a minimum two year follow-up. There were two superficial wound infections. One patient developed a non-union which required a formal open procedure. Another patient was symptomatic from a palpable plate inferiorly. An excellent AOFAS result was obtained in 83% (20\\/24) of the patients. Early minimally invasive reduction and fixation of complex high velocity pilon fractures gave very satisfactory results at a minimum of two years follow-up.

  6. Operative management of closed intra-articular fractures of distal end of humerus in adults.

    Science.gov (United States)

    Bhattacharyya, Arunangsu; Jha, Amrish Kumar; Chatterjee, Debdutta; Ghosh, Bappaditya; Roy, Sandip Kumar; Banerjee, Debabrata

    2011-06-01

    This is a study of 60 patients with mean age of 30 years having intra-articular fracture of distal humerus of type C (AO classification). We treated these fractures by open reduction and internal fixation through transolecranon approach. Mean duration of operative time with that of injury was 10 days. In all the cases 1st the intracondylar fracture was fixed followed by reconstruction and stabilisation of medial and lateral pillar by 1/3 tubular plate and 3.5 mm dynamic compressent plate (DCP) or recon plate respectively. Minimum follow-up was of 1 year duration with average follow-up of 3 years. We could achieve 100% union with mean range of movement at the and of 1 year was 20 degree to 110 degree. Two fractures had delayed union, 5 patients had skin complications for which extended dressing was required. None of these patients required SSG or secondary suture.

  7. Technical innovation: digital tomosynthesis of the hip following intra-articular administration of contrast

    Energy Technology Data Exchange (ETDEWEB)

    Gazaille, Roland E.; Flynn, Michael J.; Page, Walter; Finley, Sonia; Holsbeeck, Marnix van [Henry Ford Hospital, Department of Musculoskeletal Radiology, Detroit, MI (United States)

    2011-11-15

    To demonstrate the clinical use of digital tomosynthesis in the depiction of labral and chondral pathology in the setting of post-operative CAM-type impingement of the hip following intra-articular administration of dilute iodinated contrast. We present images from a 46 year-old African American female with suspected CAM-type femoroacetabular impingement (FAI) following percutaneous pinning of the right hip for slipped capital femoral epiphysis (SCFE). A partial tear of the labrum and clinically significant acetabular chondral abnormalities were demonstrated with the use of digital tomosynthesis with superb anatomic detail. Digital tomosynthesis can be of great clinical utility and can depict pathology in superb anatomic detail, particularly in situations in which MRI is not available as well as under circumstances in which artifact due to orthopedic hardware is of concern as shown in this case. (orig.)

  8. Protocols for the in vitro design of animal articular cartilage based on tissue engineering methods

    Directory of Open Access Journals (Sweden)

    Diego Correa

    2012-10-01

    Full Text Available The articular cartilage is the structure that covers the joint ends. It has some specific tasks crucial to the correct joint physiology. It may experience a large amount of injuries that could generate considerable disabilities. Unfortunately its selfrepair capacity is too limited; therefore, many treatments have been developed with partial success, given the suboptimal biomechanical behavior of the resultant tissue. Given that, Tissue Engineering offers an alternative, based on the design of a new tissue with biological and biomechanical features which resembles the native tissue. In this work, the authors describe the methodologies followed to accomplish that goal, studying the chondrocytes harvesting, the cellular cultures, the scaffold seeding processes, the mechanical stimulation and the structural and biomechanical evaluation. Finally, exposed some of the preliminary results, as a experimental validation of the methods proposed are.

  9. Chondroblastoma of the femoral head disrupting the articular cartilage. Description of a novel surgical technique.

    Science.gov (United States)

    Givissis, Panagiotis; Agathangelidis, Filon; Christodoulou, Evangelos; Christodoulou, Anastasios

    2012-06-01

    Chondroblastoma is a rare benign tumour. Involvement of the femoral head may often lead to a delayed diagnosis. We present the case of a 15-year-old patient with right hip pain which was first attributed to adductor tendinitis. Following aggravation of the symptoms, thorough investigation including a CT-guided biopsy, revealed the diagnosis of chondroblastoma of the femoral head. Removal of the lesion based on the techniques described in literature was not possible, mainly because the articular cartilage was breached. A novel surgical technique was used in order to address the rare location and behaviour of the tumour. This technique offered the patient pain relief and return to his previous every day and sports activities. No recurrence was seen at two years follow-up.

  10. Elastoviscous Transitions of Articular Cartilage Reveal a Mechanism of Synergy between Lubricin and Hyaluronic Acid.

    Directory of Open Access Journals (Sweden)

    Edward D Bonnevie

    Full Text Available When lubricated by synovial fluid, articular cartilage provides some of the lowest friction coefficients found in nature. While it is known that macromolecular constituents of synovial fluid provide it with its lubricating ability, it is not fully understood how two of the main molecules, lubricin and hyaluronic acid, lubricate and interact with one another. Here, we develop a novel framework for cartilage lubrication based on the elastoviscous transition to show that lubricin and hyaluronic acid lubricate by distinct mechanisms. Such analysis revealed nonspecific interactions between these molecules in which lubricin acts to concentrate hyaluronic acid near the tissue surface and promotes a transition to a low friction regime consistent with the theory of viscous boundary lubrication. Understanding the mechanics of synovial fluid not only provides insight into the progression of diseases such as arthritis, but also may be applicable to the development of new biomimetic lubricants.

  11. Elastoviscous Transitions of Articular Cartilage Reveal a Mechanism of Synergy between Lubricin and Hyaluronic Acid.

    Science.gov (United States)

    Bonnevie, Edward D; Galesso, Devis; Secchieri, Cynthia; Cohen, Itai; Bonassar, Lawrence J

    2015-01-01

    When lubricated by synovial fluid, articular cartilage provides some of the lowest friction coefficients found in nature. While it is known that macromolecular constituents of synovial fluid provide it with its lubricating ability, it is not fully understood how two of the main molecules, lubricin and hyaluronic acid, lubricate and interact with one another. Here, we develop a novel framework for cartilage lubrication based on the elastoviscous transition to show that lubricin and hyaluronic acid lubricate by distinct mechanisms. Such analysis revealed nonspecific interactions between these molecules in which lubricin acts to concentrate hyaluronic acid near the tissue surface and promotes a transition to a low friction regime consistent with the theory of viscous boundary lubrication. Understanding the mechanics of synovial fluid not only provides insight into the progression of diseases such as arthritis, but also may be applicable to the development of new biomimetic lubricants.

  12. [Arthroscopic management of intra-articular fractures of the distal radius].

    Science.gov (United States)

    Cognet, J-M; Martinache, X; Mathoulin, C

    2008-09-01

    The use of arthroscopy in the management of intra-articular fractures of the distal radius has become established over the last ten years, but the operative technique is not yet standardised. We report our experience with this technique and give a stage by stage description of the operative procedure. The arthroscopic part of the procedure consists firstly of an evaluation of the bony, cartilaginous and ligamentous injuries and secondly direct visual control of the reduction. The choice of bone fixation depends on the individual preferences of the surgeon but may be influenced by the configuration of the fracture. A literature review reiterates the advantages of arthroscopic assistance in managing these fractures without revealing any disadvantages. However, mastery of the arthroscopic techniques is vital before the full advantages of this type of management can be realised. PMID:18774328

  13. Flavonoid Compound Icariin Activates Hypoxia Inducible Factor-1α in Chondrocytes and Promotes Articular Cartilage Repair.

    Science.gov (United States)

    Wang, Pengzhen; Zhang, Fengjie; He, Qiling; Wang, Jianqi; Shiu, Hoi Ting; Shu, Yinglan; Tsang, Wing Pui; Liang, Shuang; Zhao, Kai; Wan, Chao

    2016-01-01

    Articular cartilage has poor capability for repair following trauma or degenerative pathology due to avascular property, low cell density and migratory ability. Discovery of novel therapeutic approaches for articular cartilage repair remains a significant clinical need. Hypoxia is a hallmark for cartilage development and pathology. Hypoxia inducible factor-1alpha (HIF-1α) has been identified as a key mediator for chondrocytes to response to fluctuations of oxygen availability during cartilage development or repair. This suggests that HIF-1α may serve as a target for modulating chondrocyte functions. In this study, using phenotypic cellular screen assays, we identify that Icariin, an active flavonoid component from Herba Epimedii, activates HIF-1α expression in chondrocytes. We performed systemic in vitro and in vivo analysis to determine the roles of Icariin in regulation of chondrogenesis. Our results show that Icariin significantly increases hypoxia responsive element luciferase reporter activity, which is accompanied by increased accumulation and nuclear translocation of HIF-1α in murine chondrocytes. The phenotype is associated with inhibiting PHD activity through interaction between Icariin and iron ions. The upregulation of HIF-1α mRNA levels in chondrocytes persists during chondrogenic differentiation for 7 and 14 days. Icariin (10-6 M) increases the proliferation of chondrocytes or chondroprogenitors examined by MTT, BrdU incorporation or colony formation assays. Icariin enhances chondrogenic marker expression in a micromass culture including Sox9, collagen type 2 (Col2α1) and aggrecan as determined by real-time PCR and promotes extracellular matrix (ECM) synthesis indicated by Alcian blue staining. ELISA assays show dramatically increased production of aggrecan and hydroxyproline in Icariin-treated cultures at day 14 of chondrogenic differentiation as compared with the controls. Meanwhile, the expression of chondrocyte catabolic marker genes

  14. Flavonoid Compound Icariin Activates Hypoxia Inducible Factor-1α in Chondrocytes and Promotes Articular Cartilage Repair.

    Directory of Open Access Journals (Sweden)

    Pengzhen Wang

    Full Text Available Articular cartilage has poor capability for repair following trauma or degenerative pathology due to avascular property, low cell density and migratory ability. Discovery of novel therapeutic approaches for articular cartilage repair remains a significant clinical need. Hypoxia is a hallmark for cartilage development and pathology. Hypoxia inducible factor-1alpha (HIF-1α has been identified as a key mediator for chondrocytes to response to fluctuations of oxygen availability during cartilage development or repair. This suggests that HIF-1α may serve as a target for modulating chondrocyte functions. In this study, using phenotypic cellular screen assays, we identify that Icariin, an active flavonoid component from Herba Epimedii, activates HIF-1α expression in chondrocytes. We performed systemic in vitro and in vivo analysis to determine the roles of Icariin in regulation of chondrogenesis. Our results show that Icariin significantly increases hypoxia responsive element luciferase reporter activity, which is accompanied by increased accumulation and nuclear translocation of HIF-1α in murine chondrocytes. The phenotype is associated with inhibiting PHD activity through interaction between Icariin and iron ions. The upregulation of HIF-1α mRNA levels in chondrocytes persists during chondrogenic differentiation for 7 and 14 days. Icariin (10-6 M increases the proliferation of chondrocytes or chondroprogenitors examined by MTT, BrdU incorporation or colony formation assays. Icariin enhances chondrogenic marker expression in a micromass culture including Sox9, collagen type 2 (Col2α1 and aggrecan as determined by real-time PCR and promotes extracellular matrix (ECM synthesis indicated by Alcian blue staining. ELISA assays show dramatically increased production of aggrecan and hydroxyproline in Icariin-treated cultures at day 14 of chondrogenic differentiation as compared with the controls. Meanwhile, the expression of chondrocyte catabolic

  15. Gene Modification of Mesenchymal Stem Cells and Articular Chondrocytes to Enhance Chondrogenesis

    Directory of Open Access Journals (Sweden)

    Saliya Gurusinghe

    2014-01-01

    Full Text Available Current cell based treatment for articular cartilage and osteochondral defects are hampered by issues such as cellular dedifferentiation and hypertrophy of the resident or transplanted cells. The reduced expression of chondrogenic signalling molecules and transcription factors is a major contributing factor to changes in cell phenotype. Gene modification of chondrocytes may be one approach to redirect cells to their primary phenotype and recent advances in nonviral and viral gene delivery technologies have enabled the expression of these lost factors at high efficiency and specificity to regain chondrocyte function. This review focuses on the various candidate genes that encode signalling molecules and transcription factors that are specific for the enhancement of the chondrogenic phenotype and also how epigenetic regulators of chondrogenesis in the form of microRNA may also play an important role.

  16. Management of extra-articular fractures of the distal tibia: intramedullary nailing versus plate fixation.

    Science.gov (United States)

    Casstevens, Chris; Le, Toan; Archdeacon, Michael T; Wyrick, John D

    2012-11-01

    Intramedullary nailing and plate fixation represent two viable approaches to internal fixation of extra-articular fractures of the distal tibia. Although both techniques have demonstrated success in maintaining reduction and promoting stable union, they possess distinct advantages and disadvantages that require careful consideration during surgical planning. Differences in soft-tissue health and construct stability must be considered when choosing between intramedullary nailing and plating of the distal tibia. Recent advances in intramedullary nail design and plate-and-screw fixation systems have further increased the options for management of these fractures. Current evidence supports careful consideration of the risk of soft-tissue complications, residual knee pain, and fracture malalignment in the context of patient and injury characteristics in the selection of the optimal method of fixation.

  17. Experiencia clínica del uso de la tepoxalina en el tratamiento conservativo de la ruptura del ligamento cruzado craneal en perros

    Directory of Open Access Journals (Sweden)

    Alexandre L. de Andrade

    2009-12-01

    Full Text Available La ruptura del ligamento cruzado craneal (RLCC se caracteriza por la laceración parcial o total del ligamento cruzado craneal. Pacientes con laceraciones agudas del ligamento (parcial o total presentan claudicación aguda con incapacidad total o parcial de sustentación del peso en el miembro afectado. La terapia clínica con principios conservadores debe ser empleada previamente al tratamiento quirúrgico. La tepoxalina es un antiinflamatorio no esteroidal, indicado en la reducción de procesos inflamatorios y alivio del dolor causado por perturbaciones músculo-esqueléticas agudas y crónicas. Este medicamento promueve el bloqueo balanceado de la ruptura del ácido araquidónico por las vías de la lipoxigenasa y cicloxigenasa de la cascada de la inflamación. El presente trabajo tiene por objetivo evaluar la eficacia clínica del uso de la tepoxalina en el tratamiento conservativo de la RLLC, por medio de test clínicos específicos, examen radiográfico y análisis citológico del líquido articular. Diez perros con RLCC, machos y hembras, fueron incluidos en este estudio y recibieron dosis diarias de 10 mg/kg de tepoxalina durante veintiocho días. La evaluación de la claudicación, los test de arco de movimiento en extensión máxima y dígito-presión, así como el análisis del líquido articular después del período de tratamiento, permitieron concluir la eficacia de la tepoxalina en el tratamiento conservativo de la RLCC. Su uso posibilitó el retorno a la deambulación normal en todos los casos tratados, siendo considerada una opción más de AINE para el tratamiento conservativo de esta artropatía.

  18. Of mice, men and elephants: the relation between articular cartilage thickness and body mass.

    Directory of Open Access Journals (Sweden)

    Jos Malda

    Full Text Available Mammalian articular cartilage serves diverse functions, including shock absorption, force transmission and enabling low-friction joint motion. These challenging requirements are met by the tissue's thickness combined with its highly specific extracellular matrix, consisting of a glycosaminoglycan-interspersed collagen fiber network that provides a unique combination of resilience and high compressive and shear resistance. It is unknown how this critical tissue deals with the challenges posed by increases in body mass. For this study, osteochondral cores were harvested post-mortem from the central sites of both medial and lateral femoral condyles of 58 different mammalian species ranging from 25 g (mouse to 4000 kg (African elephant. Joint size and cartilage thickness were measured and biochemical composition (glycosaminoclycan, collagen and DNA content and collagen cross-links densities were analyzed. Here, we show that cartilage thickness at the femoral condyle in the mammalian species investigated varies between 90 µm and 3000 µm and bears a negative allometric relationship to body mass, unlike the isometric scaling of the skeleton. Cellular density (as determined by DNA content decreases with increasing body mass, but gross biochemical composition is remarkably constant. This however need not affect life-long performance of the tissue in heavier mammals, due to relatively constant static compressive stresses, the zonal organization of the tissue and additional compensation by joint congruence, posture and activity pattern of larger mammals. These findings provide insight in the scaling of articular cartilage thickness with body weight, as well as in cartilage biochemical composition and cellularity across mammalian species. They underscore the need for the use of appropriate in vivo models in translational research aiming at human applications.

  19. Articular soft tissue anatomy of the archosaur hip joint: Structural homology and functional implications.

    Science.gov (United States)

    Tsai, Henry P; Holliday, Casey M

    2015-06-01

    Archosaurs evolved a wide diversity of locomotor postures, body sizes, and hip joint morphologies. The two extant archosaurs clades (birds and crocodylians) possess highly divergent hip joint morphologies, and the homologies and functions of their articular soft tissues, such as ligaments, cartilage, and tendons, are poorly understood. Reconstructing joint anatomy and function of extinct vertebrates is critical to understanding their posture, locomotor behavior, ecology, and evolution. However, the lack of soft tissues in fossil taxa makes accurate inferences of joint function difficult. Here, we describe the soft tissue anatomies and their osteological correlates in the hip joint of archosaurs and their sauropsid outgroups, and infer structural homology across the extant taxa. A comparative sample of 35 species of birds, crocodylians, lepidosaurs, and turtles ranging from hatchling to skeletally mature adult were studied using dissection, imaging, and histology. Birds and crocodylians possess topologically and histologically consistent articular soft tissues in their hip joints. Epiphyseal cartilages, fibrocartilages, and ligaments leave consistent osteological correlates. The archosaur acetabulum possesses distinct labrum and antitrochanter structures on the supraacetabulum. The ligamentum capitis femoris consists of distinct pubic- and ischial attachments, and is homologous with the ventral capsular ligament of lepidosaurs. The proximal femur has a hyaline cartilage core attached to the metaphysis via a fibrocartilaginous sleeve. This study provides new insight into soft tissue structures and their osteological correlates (e.g., the antitrochanter, the fovea capitis, and the metaphyseal collar) in the archosaur hip joint. The topological arrangement of fibro- and hyaline cartilage may provide mechanical support for the chondroepiphysis. The osteological correlates identified here will inform systematic and functional analyses of archosaur hindlimb evolution and

  20. Healing results in meniscus and articular cartilage photochemically welded with 1,8-naphthalimide dyes

    Science.gov (United States)

    Judy, Millard M.; Jackson, Robert W.; Nosir, Hany R.; Matthews, James Lester; Loyd, John D.; Lewis, David E.; Utecht, Ronald E.; Yuan, Dongwu

    1997-05-01

    Meniscal tears and partial thickness defects in articular cartilage do not heal spontaneously. In this paper results are described of studies of a procedure for evoking the healing response in such lesions by a non-thermal tissue sparing photochemical weld using 1,8-naphthalimide dyes. Fifteen essentially mature Barbados sheep 40 - 60 pounds in weight received a 2 - 3 mm flap tear by incision in the red white zone of the medial meniscus oriented parallel to the table of the tibia. The animals were divided into four groups; Group I, no treatment; Group II, treatment by laser activated photoactive dyes; Group III, treatment by suturing; Group IV, treatment by laser irradiation only; Group V, treatment by photoactive dyes only. In another group of 12 sheep partial thickness flap tear was created by incision in the articular cartilage of the femoral condyle. These were divided into four groups as for the meniscus study, omitting the sutured control. Welds were made using the dimeric dye MBM Gold BW 012-012-012 at 12 mM in PBS, 457.9 nm argon ion laser radiation at 800 mW/cm2, 7.5 minutes (360 J/cm2) with approximately 2 kg/cm2 externally applied pressure. Animals were sacrificed at 24 hr, 4 weeks, 3 and 6 months postoperatively. Gross appearance of menisci and cartilage in all welded knees was normal and all welds resisted deformation or loosening under forceful probing. Histology of studies of both tissues out to 6 moths disclosed close bonding of welded area, continuing healing response in the form of cellular recruitment and protein deposition and the absence of inflammatory response. Tissue erosion and arthritic changes were evident in all unwelded controls.

  1. Gold Nanoparticles of Diameter 13 nm Induce Apoptosis in Rabbit Articular Chondrocytes.

    Science.gov (United States)

    Huang, Hao; Quan, Ying-Yao; Wang, Xiao-Ping; Chen, Tong-Sheng

    2016-12-01

    Gold nanoparticles (AuNPs) have been widely used in biomedical science including antiarthritic agents, drug loading, and photothermal therapy. In this report, we studied the effects of AuNPs with diameters of 3, 13, and 45 nm, respectively, on rabbit articular chondrocytes. AuNPs were capped with citrate and their diameter and zeta potential were measured by dynamic light scattering (DLS). Cell viability was evaluated by Cell Counting Kit-8 (CCK-8) assay after the rabbit articular chondrocytes were pre-incubated with 3, 13, and 45 nm AuNPs, respectively, for 24 h. Flow cytometry (FCM) analysis with annexin V/propidium iodide (PI) double staining and fluorescence imaging with Hoechst 33258 staining were used to determine the fashion of AuNPs-induced chondrocyte death. Further, 13 nm AuNPs (2 nM) significantly induced chondrocyte death accompanying apoptotic characteristics including mitochondrial damage, externalization of phosphatidylserine and nuclear concentration. However, 3 nm AuNPs (2 nM) and 45 nm (0.02 nM) AuNPs did not induce cytotoxicity in chondrocytes. Although 13 nm AuNPs (2 nM) increased the intracellular reactive oxygen species (ROS) level, pretreatment with Nacetyl cysteine (NAC), a ROS scavenger, did not prevent the cytotoxicity induced by 13 nm AuNPs, indicating that 13 nm AuNPs (2 nM) induced ROS-independent apoptosis in chondrocytes. These results demonstrate the size-dependent cytotoxicity of AuNPs in chondrocytes, which must be seriously considered when using AuNPs for treatment of osteoarthritis (OA). PMID:27178054

  2. Effect of nitric oxide synthase inhibitor on proteoglycan metabolism in repaired articular cartilage in rabbits

    Institute of Scientific and Technical Information of China (English)

    孙炜; 金大地; 王吉兴; 秦立赟; 刘晓霞

    2003-01-01

    Objective: To study the effect of nitric oxide synthase inhibitor, S-methyl thiocarbamate (SMT), on proteoglycan metabolism in repaired articular cartilage in rabbits. Methods: Twenty-four male New Zealand white rabbits, aged 8 months and weighing 2.5 kg±0.2 kg, were used in this study. Cartilage defects in full thickness were created on the intercondylar articular surface of bilateral femurs of all the rabbits. Then the rabbits were randomly divided into 3 groups (n=8 in each group). The defects in one group were filled with fibrin glue impregnated with recombinant human bone morphogenetic protein-2 (rhBMP-2, BMP group), in one group with fibrin glue impregnated with rhBMP-2 and hypodermic injection with SMT (SMT group) and in the other group with nothing (control group). All the animals were killed at one year postoperatively. The tissue sections were stained with safranine O-fast green and analyzed by Quantiment 500 system to determine the content of glycosaminoglycan through measuring the percentage of safranine O-stained area, the thickness of cartilages and the mean gray scale (average stain intensity). Radiolabelled sodium sulphate (Na235SO4) was used to assess the proteoglycan synthesis. Results: At one year postoperatively, the percentage of safranine O-stained area, the mean gray scale and the cartilage thickness of the repaired tissues in SMT group were significantly higher than those of BMP group (P<0.01) and the control group (P<0.05). Result of incorporation of Na235SO4 showed that the proteoglycan synthesis in SMT group was higher than those of BMP group and the control group (P<0.01). Conclusions: SMT, a nitric oxide synthase inhibitor, can significantly increase the content of glycosaminoglycan and proteoglycan synthesis, and computer-based image analysis is a reliable method for evaluating proteoglycan metabolism.

  3. Gold Nanoparticles of Diameter 13 nm Induce Apoptosis in Rabbit Articular Chondrocytes

    Science.gov (United States)

    Huang, Hao; Quan, Ying-yao; Wang, Xiao-ping; Chen, Tong-sheng

    2016-05-01

    Gold nanoparticles (AuNPs) have been widely used in biomedical science including antiarthritic agents, drug loading, and photothermal therapy. In this report, we studied the effects of AuNPs with diameters of 3, 13, and 45 nm, respectively, on rabbit articular chondrocytes. AuNPs were capped with citrate and their diameter and zeta potential were measured by dynamic light scattering (DLS). Cell viability was evaluated by Cell Counting Kit-8 (CCK-8) assay after the rabbit articular chondrocytes were pre-incubated with 3, 13, and 45 nm AuNPs, respectively, for 24 h. Flow cytometry (FCM) analysis with annexin V/propidium iodide (PI) double staining and fluorescence imaging with Hoechst 33258 staining were used to determine the fashion of AuNPs-induced chondrocyte death. Further, 13 nm AuNPs (2 nM) significantly induced chondrocyte death accompanying apoptotic characteristics including mitochondrial damage, externalization of phosphatidylserine and nuclear concentration. However, 3 nm AuNPs (2 nM) and 45 nm (0.02 nM) AuNPs did not induce cytotoxicity in chondrocytes. Although 13 nm AuNPs (2 nM) increased the intracellular reactive oxygen species (ROS) level, pretreatment with Nacetyl cysteine (NAC), a ROS scavenger, did not prevent the cytotoxicity induced by 13 nm AuNPs, indicating that 13 nm AuNPs (2 nM) induced ROS-independent apoptosis in chondrocytes. These results demonstrate the size-dependent cytotoxicity of AuNPs in chondrocytes, which must be seriously considered when using AuNPs for treatment of osteoarthritis (OA).

  4. Reconstruction of the anterior cruciate ligament: comparison of analgesia using intrathecal morphine, intra-articular morphine and intra-articular levobupivacaine

    Directory of Open Access Journals (Sweden)

    Leandro Queiroz Pinheiro

    2015-06-01

    Full Text Available OBJECTIVE: To compare the analgesic effect of intra-articular administration of morphine and levobupivacaine (separately or in combination with intrathecal administration of morphine in patients undergoing anterior cruciate ligament (ACL reconstruction using autologous grafts from the patellar tendon.METHODS: This was a retrospective analysis on data gathered from the medical files of 60 patients aged 20 to 50 years who underwent knee video arthroscopy for ACL reconstruction. The patients were divided into four groups of 15 individuals (A, B, C and D according to the agent administered into the joint and around the incision: 20 mL of saline solution with 5 mg of morphine in A; 20 mL of 0.5% levobupivacaine solution in B; 10 mL of solution with 2.5 mg of morphine plus 10 mL of 0.5% levobupivacaine solution in C; and morphine administered intrathecally in D.RESULTS: All the groups presented low pain scores during the first 12 h after the surgery. Groups B and C presented significantly greater pain scores than shown by group D (control, 24 h after the surgery. There was no statistical difference in pain scores between group A and group D.CONCLUSION: The patients in group A presented analgesia comparable to that of the patients in group D, whereas the procedure of group C was no capable of reproducing the analgesic effect observed in group D, as observed 24 h after the surgery. Further studies are needed in order to show the exact mechanism of action, along with the ideal dose and concentration for applying opioids to joints.

  5. del cambio

    Directory of Open Access Journals (Sweden)

    Alejandro Canales

    2006-01-01

    Full Text Available El artículo analiza las propuestas y los compromisos en materia de cobertura y de financiamiento en el periodo 2000-2006. En particular, revisa las modificaciones y los ajustes de los tres principales documentos en los que se expresaron las iniciativas: el de la Asociación Nacional de Universidades e Instituciones de Educación Superior, el del equipo de la transición y el programa sectorial. Los resultados muestran que las cifras de atención a la demanda educativa e inversión quedaron lejos de las metas autoimpuestas por el gobierno federal y que será necesario realizar mayores esfuerzos para atender tales desafíos.

  6. Reacción aguda local tras infiltración intraarticular con Synvisc (Hylan GF 20: A propósito de dos casos Acute local reaction to intra-articular infiltration with Synvisc (Hylan GF20: About two cases

    Directory of Open Access Journals (Sweden)

    E. Noáin

    2003-08-01

    Full Text Available Un segundo escalón en el tratamiento de la artrosis después de los antiinflamatorios no esteroideos son los denominados condroprotectores y la viscosuplementación intraarticular con ácido hialurónico empleado generalmente en la rodilla. Aunque poco frecuente hay descritos casos de artritis inflamatoria transitoria tras su administración intraarticular. El principal problema es el diagnóstico diferencial con una artritis séptica secundaria y sus consecuencias. Se trata de un proceso generalmente benigno y transitorio de etiología aún desconocida con diversas hipótesis pero que conlleva la suspensión del tratamiento. Presentamos dos casos que resolvieron sin secuelas con diferentes momentos de aparición, el primero en las tres horas siguientes a la infiltración y el segundo cuatro días después.A second stage in the treatment of arthrosis following the non-steroid anti-inflammatories is formed by the so-called chondroprotectors and intraarticular viscosupplementation with hyaluronic acid, generally in the knee. Although infrequent, cases have been described of transitory inflammatory arthritis following intra-articulary administration. The main problem is differential diagnosis with a septic secondary arthritis and its consequences. This is a generally benign process with a still unknown transitory aetiology with different hypotheses, but which involves suspension of the treatment. We present two cases that resolved the sequels with different moments of appearance, the first in the three hours following infiltration, and the second four days later.

  7. DOCUMENTO: FUNCIÓN Y PROFESIONALIDAD DEL TRABAJO SOCIAL

    Directory of Open Access Journals (Sweden)

    Cristina De Robertis

    2009-08-01

    Full Text Available Desde su surgimiento a fines del siglo XIX, la historia del Trabajo Social francés nos muestra períodos de creación y de transformación de la profesión. Durante las guerras del siglo XX los trabajadores sociales desplegaron capacidad de adaptación y de resistencia en esos períodos tan difíciles, sometidos a tensiones y decisiones éticas importantes. Después de Mayo del 68, el Trabajo Social fué criticado y analizado en términos de normalización y de control de la población, en su rol, según Althuser, de “aparato ideológico de Estado”. Pero el trabajo social asume también otras funciones, puesto que el costo del sufrimiento social es importante tanto en el plano económico que humano. Los trabajadores sociales invierten esfuerzo en mejorar las condiciones de vida de las personas y de los grupos y en luchar por mayor justicia social. El Trabajo Social enfrenta presiones contradictorias que debe asumir apoyándose en los valores éticos, en la deontología y en sus conocimientos teóricos y prácticos. Puede entonces articular las tres dimensiones del saber, saber hacer y saber ser (savoir, savoir faire, savoir être. La profesionalidad se construye en tensión constante entre las fuerzas internas y externas, entre los elementos de permanencia y los de cambio.

  8. Incongruent reduction following post-traumatic hip dislocations as an indicator of intra-articular loose bodies: A prospective study of 117 dislocations

    Directory of Open Access Journals (Sweden)

    K Karthik

    2011-01-01

    Conclusions: Intra-articular loose bodies were identified by nonconcentric reduction in 12 out of 117 patients with simple hip dislocation. Careful evaluation by fluoroscopy and good quality radiographs are indicated following reduction of hip dislocations.

  9. Valoración médico-laboral del trabajador con Síndrome de Sjögren

    Directory of Open Access Journals (Sweden)

    Maider Usarbarrena Ekiza

    2014-03-01

    Full Text Available El Síndrome de Sjögren (SS es una patología inflamatoria crónica, infradiagnosticada y de etiología desconocida, que se caracteriza por la infiltración linfocitaria de las glándulas exocrinas. La sintomatología puede ser muy variada. Inicialmente, lo más común es que se presente sequedad de mucosas (principalmente oculares y bucales; también se puede asociar a otras enfermedades autoinmunes, y hasta en el 65% de los casos, se pueden presentar manifestaciones extraglandulares, pudiéndose afectar cualquier parte del organismo. En muchos casos, la sintomatología inicial es leve, achacándose habitualmente a un posible disconfort en el puesto de trabajo. A esto hay que añadir que a nivel laboral no existe literatura específica en la que poder basarse para realizar una adecuada valoración médico-laboral. El objetivo de este trabajo ha sido crear una herramienta que englobe, por una parte, la vigilancia de la salud específica, y por otra, definir (aunque sea de forma orientativa, los criterios para poder realizar una correcta valoración de la capacidad laboral en los trabajadores con Síndrome de Sjögren.

  10. Specificity of Fourier Transform Infrared (FTIR) Microspectroscopy to Estimate Depth-Wise Proteoglycan Content in Normal and Osteoarthritic Human Articular Cartilage

    OpenAIRE

    Saarakkala, Simo; Julkunen, Petro

    2010-01-01

    Background: Fourier transform infrared (FTIR) microspectroscopy is a promising method for estimating the depth-wise composition of articular cartilage. The aim was to compare the specificity of two earlier introduced, presumably proteoglycan (PG)–specific FTIR parameters (i.e., absorption in the carbohydrate region with and without normalization with Amide I absorption) to estimate the reference PG content of normal and osteoarthritic human articular cartilage. This study is a direct continua...

  11. Quantitative analysis of rough endoplasmic reticulum in chondrocytes of articular and tracheal cartilage of rabbits following the systemic administration of hydrocortisone.

    OpenAIRE

    T. Itani; Kanai, K.; Watanabe, J.; Ogawa, R; Kanamura, S

    1992-01-01

    The rough endoplasmic reticulum (RER) in chondrocytes was analysed stereologically in articular cartilage of knee joints and in tracheal cartilage of rabbits injected intramuscularly with 5 mg/kg hydrocortisone daily for 4 wk. In articular cartilage, RER area per unit cytoplasmic volume decreased in chondrocytes in all (superficial, middle and deep) zones, although the volume of glycogen deposits per unit cytoplasmic volume increased in the middle and deep zones. RER area per chondrocyte also...

  12. Intra-articular sodium hyaluronate 2 mL versus physiological saline 20 mL versus physiological saline 2 mL for painful knee osteoarthritis

    DEFF Research Database (Denmark)

    Lundsgaard, C; Dufour, N; Fallentin, E;

    2008-01-01

    Methodological constraints weaken previous evidence on intra-articular viscosupplementation and physiological saline distention for osteoarthritis. We conducted a randomized, patient- and observer-blind trial to evaluate these interventions in patients with painful knee osteoarthritis.......Methodological constraints weaken previous evidence on intra-articular viscosupplementation and physiological saline distention for osteoarthritis. We conducted a randomized, patient- and observer-blind trial to evaluate these interventions in patients with painful knee osteoarthritis....

  13. Micro- and Nanomechanical Analysis of Articular Cartilage by Indentation-Type Atomic Force Microscopy: Validation with a Gel-Microfiber Composite

    OpenAIRE

    LOPARIC, Marko; Wirz, Dieter; Daniels, A. U.; Raiteri, Roberto; VanLandingham, Mark R.; Guex, Geraldine; Martin, Ivan; Aebi, Ueli; Stolz, Martin

    2010-01-01

    As documented previously, articular cartilage exhibits a scale-dependent dynamic stiffness when probed by indentation-type atomic force microscopy (IT-AFM). In this study, a micrometer-size spherical tip revealed an unimodal stiffness distribution (which we refer to as microstiffness), whereas probing articular cartilage with a nanometer-size pyramidal tip resulted in a bimodal nanostiffness distribution. We concluded that indentation of the cartilage's soft proteoglycan (PG) gel gave rise to...

  14. CT imaging in the surgical treatment of one-sided extraarticular temporomandibular joint ankylosis; Uso da tomografia computadorizada no planejamento cirurgico da anquilose temporomandibular extra-articular unilateral

    Energy Technology Data Exchange (ETDEWEB)

    Villamizar, Lenin A.; Ghirelli, Carolina de Oliveira; Baroni, Carina Outi; Ferro, Daniel; Pinto, Ana Carolina Brandao de Campos Fonseca, E-mail: carinaouti@yahoo.com.br [Universidade de Sao Paulo (USP), SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia. Dept. de Cirurgia

    2012-07-01

    Temporomandibular joint (TMJ) ankylosis is characterized by difficulty to open the mouth. The ankylosis may be articular when TJM is involved and extra-articular when other factors lead to a restriction of chewing movements without TJM involvement. Case report of one patient with unilateral ankylosis and his surgical planning through the tomography imaging. CT imaging helps preoperative planning because it can locate the exact point of injury and can make the difference between the true and false ankylosis of the TJM. (author)

  15. FIXATION OF ADULT EXRTA –ARTICULAR DISTAL THIRD DIA PHYSEAL FRACTURE OF HUMERUS-A PROSPECTIVE STUDY

    Directory of Open Access Journals (Sweden)

    Ahmed

    2013-05-01

    Full Text Available ABSTRACT : Distal humerus fracture in adult is very difficul t to treat. It is very difficult to achieve alignment, anatomical reduction, rigid fixation, im mediate mobilization and fracture union in such patient MATERIAL AND METHOD-We operate 41 patient o f close extra articular fracture of distal third of humerus, using posterior approach. 10 case s were fixed with single locking plate,6 cases by single distal multiple hole plate,12 cases by two r econstruction plate,6 cases by simple T plate,7case s by oblique metaphyseal locking plate. RESULT- Doubl e plating with reconstruction plates provide a more rigid fixation than a single-locked plate. CON CLUSION- Fixation of extra articular distal humerus, diaphyseal fracture in adult are better w ith double plating than single plating, which provide more rigid fixation with early mobilization and higher mayo elbow score

  16. Alternative technique of cervical spinal stabilization employing lateral mass plate and screw and intra-articular spacer fixation

    Directory of Open Access Journals (Sweden)

    Atul Goel

    2013-01-01

    Full Text Available Aim: The author discusses an alternative technique of segmental cervical spinal fixation. Material and Methods: The subtleties of the technique are discussed on the basis of experience with 3 cases with a follow-up of between 30 and 36 months. Technique: The technique involves debridement of facetal articular cartilage, distraction of facets, jamming of ′Goel spacer′ into the articular cavity and fortification of the fixation by lateral mass plate and screw fixation. The ′double-insurance′ method of fixation is safe for vertebral artery, nerve roots and spinal neural structures and the fixation is strong. Conclusions: The discussed technique is safe and provides a strong fixation and a ground for ultimate arthrodesis.

  17. A novel computational modelling to describe the anisotropic, remodelling and reorientation behaviour of collagen fibrres in articular cartilage

    CERN Document Server

    Cortez, S; Alves, J L

    2016-01-01

    In articular cartilage the orientation of collagen fibres is not uniform, varying mostly with the depth on the tissue. Besides, the biomechanical response of each layer of the articular cartilage differs from the neighbouring ones, evolving through thickness as a function of the distribution, density and orientation of the collagen fibres. Based on a finite element implementation, a new continuum formulation is proposed to describe the remodelling and reorientation of the collagen fibres under arbitrary mechanical loads: the cartilaginous tissue is modelled based on a hyperelastic formulation, being the ground isotropic matrix described by a neo-Hookean law and the fibrillar anisotropic part modelled by a new anisotropic formulation introduced for the first time in the present work, in which both reorientation and remodelling are taken into account. To characterize the orientation of fibres, a structure tensor is defined to represent the expected distribution and orientation of fibres around a reference direc...

  18. Biomechanical research of joint III. An experimental biomechanical research on the Femur's articular surface of knee of pongidae

    Science.gov (United States)

    Renxiang, Zhang; Zuyun, Lan; Wenji, Qu

    1986-06-01

    In this paper, moiré contour fringes is applied to study the femur's articular surface of the knee of Pongidae. The preliminary division of the femur's articular surface of knee into three types is proposed. The moiré contour fringes ofthe medial condyle is taken as a mark according to the references. Owing to the fact that the moiré contour fringes obtained from experiments after the 2nd order of fringe basically follow a certain rule, an investigation is made on the distribution of the angle α which is defined as the angle of the major axis of the 2nd order's near-oval shaped moiré contour fringe on the medial condyle with the horizontal axis. Preliminary distribution graphs are given in the paper.

  19. Reduced articular cartilage thickness in joints without a history of active arthritis in children with juvenile idiopathic arthritis

    DEFF Research Database (Denmark)

    Pradsgaard, Dan Østergaard; Spannow, Anne Helene; Heuck, Carsten;

    ,49 years (5-15), girls/boys (68/27). Subsets of JIA was: 14 systemic onsets, 8 RF-positive and 23 RF-negative poly articular JIA, 18 extended and 32 persistent oligo articular JIA. In total we investigated 941 joints with grey-scale US: 186 knee joints, 188 ankle joints, 188 wrist joints, 190 2ndMCP joints.......001), Wrist joint: 0.44 mm (0.15-0.74 mm, p=0.003), and 2nd PIP-joint: 0.37 mm (0.27-0.48 mm; pankle joint and 2nd MCP joint. Within the JIA group we found no significant difference between joints with or without a history of activity, but estimates pointed towards...... for the ankle- and 2nd MCP-joints. Our results suggest that even though a joint has never been directly affected by inflammatory activity, it may be negatively influenced by the inflammatory process in general....

  20. Efecto de la ventilación mecánica con alto flujo inspiratorio sobre la reacción inflamatoria en pacientes con síndrome de distrés respiratorio

    OpenAIRE

    Villanueva Gómez, Nidia Fernanda

    2015-01-01

    El manejo del SDRA sigue siendo complejo, ya que hasta la fecha no existe ningún tratamiento eficaz, siendo la ventilación mecánica el único tratamiento de soporte. Como parte de una estrategia ventilatoria protectora, se ha preconizado el empleo de modos ventilatorios limitados por presión (PCV), que administran elevados flujos inspiratorios, pero que, en estudios experimentales se han relacionado con mayor lesión pulmonar inducida por la ventilación mecánica (VILI). HIPÓTESIS Y OB...