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Sample records for archival samples obtained

  1. Archival bone marrow samples

    DEFF Research Database (Denmark)

    Lund, Bendik; Najmi, Laeya A; Wesolowska-Andersen, Agata;

    2015-01-01

    AB Archival samples represent a significant potential for genetic studies, particularly in severe diseases with risk of lethal outcome, such as in cancer. In this pilot study, we aimed to evaluate the usability of archival bone marrow smears and biopsies for DNA extraction and purification, whole...... with samples stored for 4 to 10 years. Acceptable call rates for SNPs were detected for 7 of 42 archival samples. In conclusion, archival bone marrow samples are suitable for DNA extraction and multiple marker analysis, but WGA was less successful, especially when longer fragments were analyzed. Multiple SNP...

  2. Hot sample archiving. Revision 3

    International Nuclear Information System (INIS)

    This Engineering Study revision evaluated the alternatives to provide tank waste characterization analytical samples for a time period as recommended by the Tank Waste Remediation Systems Program. The recommendation of storing 40 ml segment samples for a period of approximately 18 months (6 months past the approval date of the Tank Characterization Report) and then composite the core segment material in 125 ml containers for a period of five years. The study considers storage at 222-S facility. It was determined that the critical storage problem was in the hot cell area. The 40 ml sample container has enough material for approximately 3 times the required amount for a complete laboratory re-analysis. The final result is that 222-S can meet the sample archive storage requirements. During the 100% capture rate the capacity is exceeded in the hot cell area, but quick, inexpensive options are available to meet the requirements

  3. Prevalence of human papillomavirus in archival samples obtained from patients with cervical pre-malignant and malignant lesions from Northeast Brazil

    Directory of Open Access Journals (Sweden)

    Prado José CM

    2010-04-01

    Full Text Available Abstract Background Human Papillomavirus (HPV is considered as a necessary, but not sufficient, cause of cervical cancer. In this study, we aimed to assess the prevalence of HPV in a series of pre-malignant and malignant cervical lesion cases, to identify the virus genotypes, and to assess their distribution pattern according to lesion type, age range, and other considered variables. The samples were submitted to histopathological revision examination and analysed by polymerase chain reaction (PCR for the presence of HPV DNA, followed by HPV typing by dot blot hybridisation. Findings Of the analysed samples, 53.7% showed pre-malignant cervical lesions, and 46.3% presented with cervical cancer. Most cancer samples (84.1% were classified as invasive carcinoma. The mean age of these cancer patients was 47.3 years. The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cancer patients. HPV 16 was the most prevalent type, followed by HPV 18 and 58, including both single and double infections. Double infection was detected in 11.6% of the samples, and the most common combination was HPV 16+18. Conclusions Cervical cancer appears to occur in women in a lower age range in the studied area, compared to the situation in other Brazilian regions. Furthermore, among the patients with CIN 3 and those with cancer, we observed a higher proportion of married women, women with more than one sexual partner, smokers, and individuals with less than an elementary education, relative to their counterparts. Findings The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cervical cancer patients from Northeast Brazil. HPV 16 was the most prevalent type, followed by HPV 18 and 58. The most common double infection was HPV 16+18. Cervical cancer appears to occur in women in a lower age range in the Northeast Brazil. Among the patients with CIN 3 and those with cancer, we observed a higher

  4. 36 CFR 1256.20 - May I obtain access to Federal archival records?

    Science.gov (United States)

    2010-07-01

    ... Federal archival records? 1256.20 Section 1256.20 Parks, Forests, and Public Property NATIONAL ARCHIVES... MATERIALS Access to Federal Archival Records § 1256.20 May I obtain access to Federal archival records? (a) Most Federal archival records are open for research without submitting a Freedom of Information...

  5. Cell sample - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open...ase Description Download License Update History of This Database Site Policy | Contact Us Cell sample - Open TG-GATEs | LSDB Archive ...

  6. Archival Bone Marrow Samples: Suitable for Multiple Biomarker Analysis?

    DEFF Research Database (Denmark)

    Lund, Bendik; Najmi, A. Laeya; Wesolowska, Agata;

    2015-01-01

    Archival samples represent a significant potential for genetic studies, particularly in severe diseases with risk of lethal outcome, such as in cancer. In this pilot study, we aimed to evaluate the usability of archival bone marrow smears and biopsies for DNA extraction and purification, whole...

  7. Archival bone marrow samples: suitable for multiple biomarker analysis.

    Science.gov (United States)

    Lund, Bendik; Najmi, Laeya A; Wesolowska-Andersen, Agata; Landsem, Veslemøy M; Rasmussen, Kirsten K; Borst, Louise; Gupta, Ramneek; Schmiegelow, Kjeld; Klungland, Helge

    2015-01-01

    AB Archival samples represent a significant potential for genetic studies, particularly in severe diseases with risk of lethal outcome, such as in cancer. In this pilot study, we aimed to evaluate the usability of archival bone marrow smears and biopsies for DNA extraction and purification, whole genome amplification (WGA), multiple marker analysis including 10 short tandem repeats, and finally a comprehensive genotyping of 33,683 single nucleotide polymorphisms (SNPs) with multiplexed targeted next-generation sequencing. A total of 73 samples from 21 bone marrow smears and 13 bone marrow biopsies from 18 Danish and Norwegian childhood acute lymphoblastic leukemia patients were included and compared with corresponding blood samples. Samples were grouped according to the age of sample and whether WGA was performed or not. We found that measurements of DNA concentration after DNA extraction was dependent on detection method and that spectrophotometry overestimated DNA amount compared with fluorometry. In the short tandem repeat analysis, detection rate dropped slightly with longer fragments. After WGA, this drop was more pronounced. Samples stored for 0 to 3 years showed better results compared with samples stored for 4 to 10 years. Acceptable call rates for SNPs were detected for 7 of 42 archival samples. In conclusion, archival bone marrow samples are suitable for DNA extraction and multiple marker analysis, but WGA was less successful, especially when longer fragments were analyzed. Multiple SNP analysis seems feasible, but the method has to be further optimized.

  8. 7 CFR 201.45 - Obtaining the working sample.

    Science.gov (United States)

    2010-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... REGULATIONS Purity Analysis in the Administration of the Act § 201.45 Obtaining the working sample. (a) The... available, the sample shall be thoroughly mixed and placed in a pile and the pile shall be...

  9. Rapid surface sampling and archival record system

    Energy Technology Data Exchange (ETDEWEB)

    Barren, E.; Penney, C.M.; Sheldon, R.B. [GE Corporate Research and Development Center, Schenectady, NY (United States)] [and others

    1995-10-01

    A number of contamination sites exist in this country where the area and volume of material to be remediated is very large, approaching or exceeding 10{sup 6} m{sup 2} and 10{sup 6} m{sup 3}. Typically, only a small fraction of this material is actually contaminated. In such cases there is a strong economic motivation to test the material with a sufficient density of measurements to identify which portions are uncontaminated, so extensively they be left in place or be disposed of as uncontaminated waste. Unfortunately, since contamination often varies rapidly from position to position, this procedure can involve upwards of one million measurements per site. The situation is complicated further in many cases by the difficulties of sampling porous surfaces, such as concrete. This report describes a method for sampling concretes in which an immediate distinction can be made between contaminated and uncontaminated surfaces. Sample acquisition and analysis will be automated.

  10. Bacterial contamination in cold water samples obtained from water dispensers.

    Science.gov (United States)

    Furuhata, Katsunori; Ishizaki, Naoto; Fukuyama, Masafumi

    2015-01-01

    We carried out a basic study in order to evaluate the bacterial contamination in water dispensers. Water samples were obtained from water dispensers from October 2012 to November 2013, and standard plate counts (at 36˚C, 24 h) of the samples, as well as heterotrophic plate counts (at 25˚C, 7 d), were estimated with the standard methods for the examination of drinking water in Japan. Standard plate counts exceeding the water-quality standard (1.0×10(2) CFU/ml) were observed in 42 of the 140 samples (30.0%), with a maximum detected bacterial count of 2.1×10(5) CFU/ml. The rate of the standard plate counts exceeding the water quality standard tended to be higher when using a one-way type method or water dispensers with natural water. Ralstonia spp. was most commonly isolated, and Pseudomonas aeruginosa was isolated in a few cases. Some opportunistic pathogens were also isolated, suggesting that we should be more concerned about bacterial contamination in cold water supplied from water dispensers.

  11. mRNA transcript quantification in archival samples using multiplexed, color-coded probes

    Directory of Open Access Journals (Sweden)

    Gullane Patrick

    2011-05-01

    Full Text Available Abstract Background A recently developed probe-based technology, the NanoString nCounter™ gene expression system, has been shown to allow accurate mRNA transcript quantification using low amounts of total RNA. We assessed the ability of this technology for mRNA expression quantification in archived formalin-fixed, paraffin-embedded (FFPE oral carcinoma samples. Results We measured the mRNA transcript abundance of 20 genes (COL3A1, COL4A1, COL5A1, COL5A2, CTHRC1, CXCL1, CXCL13, MMP1, P4HA2, PDPN, PLOD2, POSTN, SDHA, SERPINE1, SERPINE2, SERPINH1, THBS2, TNC, GAPDH, RPS18 in 38 samples (19 paired fresh-frozen and FFPE oral carcinoma tissues, archived from 1997-2008 by both NanoString and SYBR Green I fluorescent dye-based quantitative real-time PCR (RQ-PCR. We compared gene expression data obtained by NanoString vs. RQ-PCR in both fresh-frozen and FFPE samples. Fresh-frozen samples showed a good overall Pearson correlation of 0.78, and FFPE samples showed a lower overall correlation coefficient of 0.59, which is likely due to sample quality. We found a higher correlation coefficient between fresh-frozen and FFPE samples analyzed by NanoString (r = 0.90 compared to fresh-frozen and FFPE samples analyzed by RQ-PCR (r = 0.50. In addition, NanoString data showed a higher mean correlation (r = 0.94 between individual fresh-frozen and FFPE sample pairs compared to RQ-PCR (r = 0.53. Conclusions Based on our results, we conclude that both technologies are useful for gene expression quantification in fresh-frozen or FFPE tissues; however, the probe-based NanoString method achieved superior gene expression quantification results when compared to RQ-PCR in archived FFPE samples. We believe that this newly developed technique is optimal for large-scale validation studies using total RNA isolated from archived, FFPE samples.

  12. Genome-wide scans using archived neonatal dried blood spot samples

    Directory of Open Access Journals (Sweden)

    Wiuf Carsten

    2009-07-01

    Full Text Available Abstract Background Identification of disease susceptible genes requires access to DNA from numerous well-characterised subjects. Archived residual dried blood spot samples from national newborn screening programs may provide DNA from entire populations and medical registries the corresponding clinical information. The amount of DNA available in these samples is however rarely sufficient for reliable genome-wide scans, and whole-genome amplification may thus be necessary. This study assess the quality of DNA obtained from different amplification protocols by evaluating fidelity and robustness of the genotyping of 610,000 single nucleotide polymorphisms, using the Illumina Infinium HD Human610-Quad BeadChip. Whole-genome amplified DNA from 24 neonatal dried blood spot samples stored between 15 to 25 years was tested, and high-quality genomic DNA from 8 of the same individuals was used as reference. Results Using 3.2 mm disks from dried blood spot samples the optimal DNA-extraction and amplification protocol resulted in call-rates between 99.15% – 99.73% (mean 99.56%, N = 16, and conflicts with reference DNA in only three per 10,000 genotype calls. Conclusion Whole-genome amplified DNA from archived neonatal dried blood spot samples can be used for reliable genome-wide scans and is a cost-efficient alternative to collecting new samples.

  13. Immunoelectron microscopic localization of elastic tissue components in archival tissue samples.

    Science.gov (United States)

    Fanning, J C; White, J F; Polewski, R; Cleary, E G

    1991-06-01

    Tissue samples that have been stored for many years, in different media and under a variety of conditions, have been examined by modern techniques of immunoelectron microscopy, using antibodies against elastic tissue components. A range of postembedding restorative procedures has been identified, which will allow reliable immunolocalization of antibodies against the elastic tissue component of such specimens. These methods have been applied successfully to autopsy-derived material, fixed in buffered formaldehyde, to archival material stored frozen at -70 or -20 degrees C, to specimens fixed for electron microscopy and stored for many years in buffer, and even to archival material from formaldehyde-fixed, paraffin-embedded blocks, reprocessed for electron microscopic examination. The successful restorative methods included pre-treatment of the sections with 6 M guanidine hydrochloride, or 1 M Tris/saline, each containing 100 mM dithiothreitol (a reducing agent) followed by alkylation with 220 mM iodoacetamide. The application of these techniques allowed reliable study of elastic tissue antibody distributions in archival tissues that could not be obtained again, as well as comparative studies with tissues processed many years previously.

  14. A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

    DEFF Research Database (Denmark)

    Svendsen, Claus Bo; Boye, Mette; Struve, Carsten;

    2009-01-01

    A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bact......A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross...

  15. Bisulfite-Based DNA Methylation Analysis from Recent and Archived Formalin-Fixed, Paraffin Embedded Colorectal Tissue Samples.

    Science.gov (United States)

    Kalmár, Alexandra; Péterfia, Bálint; Hollósi, Péter; Wichmann, Barnabás; Bodor, András; Patai, Árpád V; Schöller, Andrea; Krenács, Tibor; Tulassay, Zsolt; Molnár, Béla

    2015-09-01

    We aimed to test the applicability of formalin-fixed and paraffin-embedded (FFPE) tissue samples for gene specific DNA methylation analysis after using two commercially available DNA isolation kits. Genomic DNA was isolated from 5 colorectal adenocarcinomas and 5 normal adjacent tissues from "recent", collected within 6 months, and "archived", collected more than 5 years ago, FFPE tissues using either High Pure FFPET DNA Isolation kit or QIAamp DNA FFPE Tissue kit. DNA methylation analysis of MAL, SFRP1 and SFRP2 genes, known to be hypermethylated in CRC, was performed using methylation-sensitive high resolution melting (MS-HRM) analysis and sequencing. QIAamp (Q) method resulted in slightly higher recovery in archived (HP: 1.22 ± 3.18 μg DNA; Q: 3.00 ± 4.04 μg DNA) and significantly (p < 0.05) higher recovery in recent samples compared to High Pure method (HP) (HP: 4.10 ± 2.91 μg DNA; Q: 11.51 ± 7.50 μg DNA). Both OD260/280 and OD260/230 ratios were lower, but still high in the High Pure isolated archived and recent samples compared to those isolated with QIAamp. Identical DNA methylation patterns were detected for all 3 genes tested by MS-HRM with both isolation kits in the recent group. However, despite of higher DNA recovery in QIAamp slightly more reproducible methylation results were obtained from High Pure isolated archived samples. Sequencing confirmed DNA hypermethylation in CRCs. In conclusion, reproducible DNA methylation patterns were obtained from recent samples using both isolation kits. However, long term storage may affect the reliability of the results leading to moderate differences between the efficiency of isolation kits.

  16. Spiral and vestibular ganglion estimates in archival temporal bones obtained by design based stereology and Abercrombie methods.

    Science.gov (United States)

    Ishiyama, Gail; Geiger, Christopher; Lopez, Ivan A; Ishiyama, Akira

    2011-03-15

    The objective of this study was to make direct comparisons of the estimates of spiral and vestibular neuronal number in human archival temporal bone specimens using design-based stereology with those using the assumption-based Abercrombie method. Archival human temporal bone specimens from subjects ranging in age from 16 to 80 years old were used. The number of spiral and vestibular ganglia neurons within the counting areas was estimated using the stereology-optical disector technique and compared with estimates obtained using the assumption-based Abercrombie method on the same specimens. Using the optical disector method, there was an average of 41,480 (coefficient of variation=0.12) spiral ganglia neurons and 28,930 (coefficient of variation=0.15) vestibular ganglia neurons. The mean coefficient of error was 0.076 for the spiral ganglion estimates, and 0.091 for the vestibular ganglion estimates. Using the Abercrombie correction method of two-dimensional analysis, an average of 23,110 (coefficient of variation of 0.08) spiral ganglia neurons, and 16,225 vestibular ganglia neurons (coefficient of variation of 0.15) was obtained. We found that there was a large disparity between the estimates with a significant 44% underestimation of the spiral and vestibular ganglion counts obtained using the Abercrombie method when compared with estimates using the optical disector method.

  17. Sample (S): SE37_S19 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available ing ShiftedIonsFinder This is a pseudo metadata prepared for integrated analyses of multiple datasets. The Medicago samples (S01, S02, and S03) and a blank sample (S16) are included. ...

  18. Sample Set (SE): SE47 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available genotype-dependent biochemical pathways. In our study of metabolite profiles we ...at the obtained clusters were significantly enriched for metabolites included in biochemical pathways

  19. Sample (S): SE37_S17 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available repared for integrated analyses of multiple datasets. The Medicago samples (S01, S02, and S03) and a blank sample (S16) are included. ... ...etection of reproducible metabolite peaks Medicago truncatula NCBI taxonomy:3880 This is a pseudo metadata p

  20. Sample Preparation (SS): SE51_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available e Master NEO, BMS, Tokyo, Japan), and the seed powder was extracted with 1 mL of extraction buffer (0.1% HCO...trifugation (4 ℃, 10,000 rpm, 5 min), the sample tubes were subjected to sample preparation (buffer transfer

  1. Archival policies and collections database for the Woods Hole Science Center's marine sediment samples

    Science.gov (United States)

    Buczkowski, Brian J.; Kelsey, Sarah A.

    2007-01-01

    The Woods Hole Science Center of the U.S. Geological Survey (USGS) has been an active member of the Woods Hole research community, Woods Hole, Massachusetts, for over 40 years. In that time there have been many projects that involved the collection of sediment samples conducted by USGS scientists and technicians for the research and study of seabed environments and processes. These samples were collected at sea or near shore and then brought back to the Woods Hole Science Center (WHSC) for analysis. While at the center, samples are stored in ambient temperature, refrigerated and freezing conditions ranging from +2º Celsius to -18º Celsius, depending on the best mode of preparation for the study being conducted or the duration of storage planned for the samples. Recently, storage methods and available storage space have become a major concern at the WHSC. The core and sediment archive program described herein has been initiated to set standards for the management, methods, and duration of sample storage. A need has arisen to maintain organizational consistency and define storage protocol. This handbook serves as a reference and guide to all parties interested in using and accessing the WHSC's sample archive and also defines all the steps necessary to construct and maintain an organized collection of geological samples. It answers many questions as to the way in which the archive functions.

  2. NUTRITIONAL AND MICROBIOLOGICAL COMPONENTS OF HONEY SAMPLES OBTAINED FROM OGUN STATE, SOUTHWESTERN NIGERIA

    Directory of Open Access Journals (Sweden)

    M. O. ADENEKAN

    2012-06-01

    Full Text Available There are no detailed studies on the nutritional and microbiological characteristics of honeyproduced in Ogun State in the Southwestern part of Nigeria. This paper investigated thesecomponents in honeys produced from different parts of this state. A total of 10 honey samples peryear were collected for the years 2008 – 2010. These were separately analyzed for their physicalproperties, nutritional and microbiological components in the laboratory. The results of thephysical properties showed that honey samples obtained from Ago-Iwoye has the lowest pH of3.48, which was significantly different from the pH values of 5.06, 5.21 and 4.06 obtained fromhoney samples from Abeokuta, Ibefun and Ilisan honey samples respectively. There wassignificant difference in moisture contents of honey samples obtained from Ogere (16.19 %, Otta(19.14 % and Ijebu-Ode (18.21 %, while the percentage ash contents of 0.78 % obtained fromhoney samples collected from Abeokuta was not significantly different from the value of 0.75 %obtained from Ago-Iwoye honey (P ≤ 0.05. However, the value of 1.11 mg 100 g-1 forhydroxymethylfurfural obtained from Ago-Iwoye honey samples was not significantly differentfrom the value of 0.32 mg 100 g-1 in honey samples obtained from Sagamu.The value for glucose ranged from 18.42 – 30.16 g 100 g-1, while fructose sugar varied between25.42 – 38.21 g 100 g-1. Minimum protein value of 0.02 % was obtained from Ijebu-Ode honey,while the maximum of 0.51 % was obtained from honey samples from Ilisan. Results of theelemental nutrient showed that potassium was the most abundant element in honey samples withthe range value of 14.78 – 17.42 mg 100 g-1 followed by calcium, which varied from 2.13 – 11.25mg 100 g-1. However, result of microbiological properties showed that the total plate count variedfrom 0.2 – 3.4 cfu g-1, whereas total coliforms were not detected in honey samples collected fromAgo-Iwoye, Otta, Ibefun, Ife and Sagamu

  3. Sample (S): SE37_S13 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available oot (aerial part) and root were separated with a scalpel and they were immediately frozen using liquid nitrogen. After lyophilization, the sample was stored at room temperature. ...

  4. Sample (S): SE37_S15 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available oot (aerial part) and root were separated with a scalpel and they were immediately frozen using liquid nitrogen. After lyophilization, the sample was stored at room temperature. ...

  5. Sample (S): SE37_S14 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available oot (aerial part) and root were separated with a scalpel and they were immediately frozen using liquid nitrogen. After lyophilization, the sample was stored at room temperature. ...

  6. Sample Set (SE): SE5 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Daiskue Shibata, Kazusa DNA Research Institute Direct Submittion version 4 ... ...s subsp. patens metabolites. 4 replicates data are examined for each sample. Norimoto Shimada, Takeshi Ara,

  7. Sample (S): SE24_S1 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available s into 1-cm squares, the samples were immediately frozen with liquid nitrogen and lyophilized at -55 C. The lyophilized materials were stored at room temperature with silica gel. ...

  8. Sample Preparation (SS): SE52_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Tokyo, Japan) using a mixer mill (MM 300, Retsch) with a zirconia bead for 6 min... at 20 Hz. Next, the samples were centrifuged at 15,000 g for 10 min and filtered (Ultrafree-MC filter, 0.2 μm; Mil...lipore, Bedford, MA, USA). The sample extracts were then applied to an HLB μElution plate (Waters, Mil

  9. Molecular strain identification of the Mycobacterium tuberculosis complex in archival tissue samples

    OpenAIRE

    Zink, A. R.; Nerlich, A G

    2004-01-01

    Aims: To investigate the use of different molecular analyses that can identify distinct strains of human pathogenic mycobacteria in formalin fixed and paraffin wax embedded archival tissue samples to see whether it is possible to differentiate between the members of the Mycobacterium tuberculosis complex (M tuberculosis, M bovis, M africanum, M microti, or M canettii) and/or substrains in a high number of samples. This would be of interest for identifying individual infection traits and super...

  10. Sample (S): SE50_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available iting both Basta and kanamycin tolerance in the F2 and F3 generations, were obtained and named WOX1-1 and WO...respectively. Progenies that exhibited both Basta and kanamycin tolerance (F2 and F3 generations) were obt...ained. Sterilized seeds were kept for 2 days at 4°C in the dark and were sown on pl

  11. Sample Preparation (SS): SE55_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available .1% acetic acid, 0.5 mg/L of lidocaine, and d-camphor sulfonic acid (Tokyo Kasei) using a mixer mill (MM 300...,000g for 10 min and filtration (Ultrafree-MC filter, 0.2 mm, Millipore), the sample extracts were applied t

  12. Sample Set (SE): SE58 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available calibration curves over a biologically defined concentration range for all detected compounds. By performing... metabolomics on a stepwise gradient between two biological specimens, we obtain ...rference. Analysis of artificial biological gradients is a general and inexpensive tool for calibration that...ments using artificial biological gradients We introduce a powerful approach that provides semiquantitative ...SE58 Exploring matrix effects and quantification performance in metabolomics experi

  13. STP K Basin Sludge Sample Archive at the Pacific Northwest National Laboratory FY2014

    Energy Technology Data Exchange (ETDEWEB)

    Fiskum, Sandra K.; Smoot, Margaret R.; Schmidt, Andrew J.

    2014-06-01

    The Pacific Northwest National Laboratory (PNNL) currently houses 88 samples (~10.5 kg) of K Basin sludge (81 wet and seven dry samples) on behalf of the Sludge Treatment Project (STP), which is managed for the U.S. Department of Energy (DOE) by the CH2M Hill Plateau Remediation Company (CHPRC). Selected samples are intended to serve, in part, as sentinels to enhance understanding of sludge properties after long-term storage, and thus enhance understanding of sludge behavior following transfer to sludge transfer and storage containers (STSCs) and storage at the Hanford 200 Area central plateau. In addition, remaining samples serve in contingency for future testing requirements. At PNNL, the samples are tracked and maintained under a prescriptive and disciplined monthly sample-monitoring program implemented by PNNL staff. This report updates the status of the K Basin archive sludge sample inventory to April 2014. The previous inventory status report, PNNL 22245 (Fiskum et al. 2013, limited distribution report), was issued in February of 2013. This update incorporates changes in the inventory related to repackaging of 17 samples under test instructions 52578 TI052, K Basin Sludge Sample Repackaging for Continued Long Term Storage, and 52578 TI053, K Basin Sludge Sample Repackaging Post-2014 Shear Strength Measurements. Note that shear strength measurement results acquired in 2014 are provided separately. Specifically, this report provides the following: • a description of the K Basin sludge sample archive program and the sample inventory • a summary and images of the samples that were repackaged in April 2014 • up-to-date images and plots of the settled density and water loss from all applicable samples in the inventory • updated sample pedigree charts, which provide a roadmap of the genesis and processing history of each sample in the inventory • occurrence and deficiency reports associated with sample storage and repackaging

  14. Effect of histologic processing on dimensions of skin samples obtained from cat cadavers.

    Science.gov (United States)

    Jeyakumar, Sakthila; Smith, Annette N; Schleis, Stephanie E; Cattley, Russell C; Tillson, D Michael; Henderson, Ralph A

    2015-11-01

    OBJECTIVE To determine changes in dimensions of feline skin samples as a result of histologic processing and to identify factors that contributed to changes in dimensions of skin samples after sample collection. SAMPLE Cadavers of 12 clinically normal cats. PROCEDURES Skin samples were obtained bilaterally from 3 locations (neck, thorax, and tibia) of each cadaver; half of the thoracic samples included underlying muscle. Length, width, and depth were measured at 5 time points (before excision, after excision, after application of ink to mark tissue margins, after fixation in neutral-buffered 10% formalin for 36 hours, and after completion of histologic processing and staining with H&E stain). Measurements obtained after sample collection were compared with measurements obtained before excision. RESULTS At the final time point, tissue samples had decreased in length (mean decrease, 32.40%) and width (mean decrease, 34.21%) and increased in depth (mean increase, 54.95%). Tissue from the tibia had the most shrinkage in length and width and that from the neck had the least shrinkage. Inclusion of underlying muscle on thoracic skin samples did not affect the degree of change in dimensions. CONCLUSIONS AND CLINICAL RELEVANCE In this study, each step during processing from excision to formalin fixation and histologic processing induced changes in tissue dimensions, which were manifested principally as shrinkage in length and width and increase in depth. Most of the changes occured during histologic processing. Inclusion of muscle did not affect thoracic skin shrinkage. Shrinkage should be a consideration when interpreting surgical margins in clinical cases. 945). PMID:26512538

  15. The use of importance sampling in a trial assessment to obtain converged estimates of radiological risk

    International Nuclear Information System (INIS)

    In developing a methodology for assessing potential sites for the disposal of radioactive wastes, the Department of the Environment has conducted a series of trial assessment exercises. In order to produce converged estimates of radiological risk using the SYVAC A/C simulation system an efficient sampling procedure is required. Previous work has demonstrated that importance sampling can substantially increase sampling efficiency. This study used importance sampling to produce converged estimates of risk for the first DoE trial assessment. Four major nuclide chains were analysed. In each case importance sampling produced converged risk estimates with between 10 and 170 times fewer runs of the SYVAC A/C model. This increase in sampling efficiency can reduce the total elapsed time required to obtain a converged estimate of risk from one nuclide chain by a factor of 20. The results of this study suggests that the use of importance sampling could reduce the elapsed time required to perform a risk assessment of a potential site by a factor of ten. (author)

  16. Characterization of biochar and bio-oil samples obtained from carbonization of various biomass materials

    Energy Technology Data Exchange (ETDEWEB)

    Oezcimen, Didem; Ersoy-Mericboyu, Ayseguel [Istanbul Technical University, Chemical-Metallurgical Engineering Faculty, Department of Chemical Engineering, Maslak 34469, Istanbul (Turkey)

    2010-06-15

    Apricot stone, hazelnut shell, grapeseed and chestnut shell are important biomass residues obtained from the food processing industry in Turkey and they have a great importance as being a source of energy. In this study, the characteristics of bio-oil and biochar samples obtained from the carbonization of apricot stone, hazelnut shell, grapeseed and chestnut shell were investigated. It was found that the biochar products can be characterized as carbon rich, high heating value and relatively pollution-free potential solid biofuels. The bio-oil products were also presented as environmentally friendly green biofuel candidates. (author)

  17. Sensitivity of the DRP-4DVar Performance to Perturbation Samples Obtained by Two Different Methods

    Institute of Scientific and Technical Information of China (English)

    ZHAO Juan; WANG Bin

    2010-01-01

    The dimension-reduced projection four-dimensional variational data assimilation (DRP-4DVar) approach utilizes the ensemble of historical forecasts to estimate the background error covariance (BEC) and directly obtains the analysis in the ensemble space. As a result, the quality of ensemble members significantly affects the DRP-4DVar performance. The historical-forecast-based initial perturbation samples are flow-dependent and can describe the error-growth pattern of the atmospheric model and the balanced relationship between different model variables. However, the ensemble spread is not big enough because of the short time interval between adjacent historical samples and the limited ensemble size. In this study, the BEC of the Weather Research and Forecasting Model (WRF) three-dimensional variational data assimilation (3DVar) system is employed to produce initial perturbation samples for the DRP-4DVar. The control variable perturbation method based on the structure characteristics of the 3DVar BEC produces initial perturbation samples that have reasonable background error correlations. Moreover, the estimated BEC also has good dynamic and physical consistency between variables after the initial perturbation samples undergo a development with a 6- or 12-h model forward integration. In terms of computational expense, the historical forecast results can be obtained without any additional computational cost at the operational numerical weather forecast centers, while the integration of samples from the 3DVar-based control variable perturbation method is time-consuming, but this difficulty can be alleviated through parallel computing. Although the assimilation run with the historical-forecast-based ensemble generates slightly better initial analysis field, the forecasts from the assimilation experiment using the 3DVar method performs better during the period from 12 to 30 h. Moreover, precipitation is simulated significantly better when the new ensemble is used. In

  18. Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement

    Directory of Open Access Journals (Sweden)

    Juliana Magalhães Vital-Brazil

    2010-05-01

    Full Text Available The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.

  19. Rapid Surface Sampling and Archival Record (RSSAR) System. Topical report, October 1, 1993--December 31, 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-06-01

    This report describes the results of Phase 1 efforts to develop a Rapid Surface Sampling and Archival Record (RSSAR) System for the detection of semivolatile organic contaminants on concrete, transite, and metal surfaces. The characterization of equipment and building surfaces for the presence of contaminants as part of building decontamination and decommissioning activities is an immensely large tacks of concern to both government and industry. Contaminated and clean materials must be clearly identified and segregated so that the clean materials can be recycled or reused, if possible, or disposed of more cheaply as nonhazardous waste. Characterization of building and equipment surfaces will be needed during initial investigations, during cleanup operations, and during the final confirmatory process, increasing the total number of samples well beyond that needed for initial characterization. This multiplicity of information places a premium on the ability to handle and track data as efficiently as possible. Aware of the shortcomings of traditional surface characterization technology, GE, with DOE support has undertaken a 12-month effort to complete Phase 1 of a proposed four-phase program to develop the RSSAR system. The objectives of this work are to provide instrumentation to cost-effectively sample concrete and steel surfaces, provide a quick-look indication for the presence or absence of contaminants, and collect samples for later, more detailed analysis in a readily accessible and addressable form. The Rapid Surface Sampling and Archival Record (RSSAR) System will be a modular instrument made up of several components: (1) sampling heads for concrete surfaces, steel surfaces, and bulk samples; (2) quick-look detectors for photoionization and ultraviolet; (3) multisample trapping module to trap and store vaporized contaminants in a manner suitable for subsequent detailed lab-based analyses.

  20. The efficacy of field techniques for obtaining and storing blood samples from fishes.

    Science.gov (United States)

    Clark, T D; Donaldson, M R; Drenner, S M; Hinch, S G; Patterson, D A; Hills, J; Ives, V; Carter, J J; Cooke, S J; Farrell, A P

    2011-11-01

    Prompted by the dramatic increase in the use of blood analyses in fisheries research and monitoring, this study investigated the efficacy of common field techniques for sampling and storing blood from fishes. Three questions were addressed: (1) Do blood samples taken via rapid caudal puncture (the 'grab-and-stab' technique) yield similar results for live v. sacrificed groups of fishes? (2) Do rapidly obtained caudal blood samples accurately represent blood properties of fishes prior to capture? (3) Does storage of whole blood in an ice slurry for a working day (8·5 h) modify the properties of the plasma? It was shown that haematocrit, plasma ions, metabolites, stress hormones and sex hormones of caudal blood samples were statistically similar when taken from live v. recently sacrificed groups of adult coho salmon Oncorhynchus kisutch. Moreover, this study confirmed by using paired blood samples from cannulated O. kisutch that blood acquired through the caudal puncture technique (mean ±s.e. 142 ± 26 s after capture) was representative of fish prior to capture. Long-term (8·5 h) cold storage of sockeye salmon Oncorhynchus nerka whole blood caused significant decreases in plasma potassium and chloride, and a significant increase in plasma glucose. Previous research has suggested that these changes largely result from net movements of ions and molecules between the plasma and erythrocytes, movements that can occur within minutes of storage. Thus, blood samples from fishes should be centrifuged as quickly as practicable in the field for separation of plasma and erythrocytes to prevent potentially misleading data. PMID:22026608

  1. Determination of radiocarbon in stratospheric CO2, obtained through AirCore sampling.

    Science.gov (United States)

    Paul, Dipayan; Chen, Huilin; Been, Henk A.; Kivi, Rigel; Meijer, Harro A. J.

    2016-04-01

    The concentration of Greenhouse Gases (GHG), with carbon dioxide as the most prominent example, has been and still is increasing, predominantly due to emissions from fossil fuel combustion. CO2 is also the most important component of the global carbon cycle. Among other tracers, radiocarbon (Carbon-14) is a unique and an important atmospheric tracer used in the understanding of the global carbon cycle. Radiocarbon is a naturally occurring isotope (radioactive, t 1/2 = 5730 ± 40 years) of carbon produced through the interaction of thermalized neutrons and nitrogen in the upper atmosphere. Generally, for performing atmospheric radiocarbon measurements in the higher atmosphere, large samples (few liters of air) were collected using aircrafts and balloons. However, collecting stratospheric samples on a regular basis for radiocarbon analysis is extremely expensive. Here we describe the determination of radiocarbon concentrations in stratospheric CO2, collected using AirCore sampling. AirCore is an innovative sampling technique for obtaining vertical atmospheric profiles and, in Europe, is done on a regular basis at Sodankylä, Finland for CO2, CH4 and CO. The stratospheric parts of two such AirCore profiles were used in this study as a proof-of-principle. CO2 from the stratospheric air samples were extracted and converted to elemental carbon, which were then measured at the Accelerator Mass Spectrometric (AMS) facility of the Centre for Isotope Research (CIO) at the University of Groningen. The stratospheric part of the AirCore profile was divided into six sections, each contained approximately 10 μg C. A detailed description of the extraction, graphitization, AMS analysis and the derivation of the stratospheric radiocarbon profile will be the main focus. Through our results, we will show that AirCore is a viable sampling method for performing high-precision radiocarbon measurements of stratospheric CO2 with reasonably good spatial resolution on a regular basis

  2. Low level electron field emission current intensities obtained from niobium samples

    International Nuclear Information System (INIS)

    We present results obtained with an experimental set-up allowing measurements of DC field emission currents in the range of 10-18 to 10-5 A. The experiments are carried out in ultra high vacuum (P-10 Torr) and at room temperature. The detection of currents in the range of 10-18-10-12 A is performed with an electron multiplier located behind a grid anode. Higher currents are measured using a bulk anode and a picoammeter. The whole system has been designed to study niobium samples from sheets used to build superconducting accelerator cavities. Measures in the low current range have shown electron emission regimes that seem not to fit the Fowler-Nordheim law. A possible mechanism for such emissions involving the surface oxide layer is proposed. Comparisons of the electron field emission from samples prepared by different surface treatments as chemical etching, electropolishing, oxide coating by anodization, are presented. (orig.)

  3. Transcriptional profiling of degraded RNA in cryopreserved and fixed tissue samples obtained at autopsy

    Directory of Open Access Journals (Sweden)

    Alhasan Samir

    2006-12-01

    Full Text Available Abstract Background Traditional multiplexed gene expression methods require well preserved, intact RNA. Such specimens are difficult to acquire in clinical practice where formalin fixation is the standard procedure for processing tissue. Even when special handling methods are used to obtain frozen tissue, there may be RNA degradation; for example autopsy samples where degradation occurs both pre-mortem and during the interval between death and cryopreservation. Although specimens with partially degraded RNA can be analyzed by qRT-PCR, these analyses can only be done individually or at low levels of multiplexing and are laborious and expensive to run for large numbers of RNA targets. Methods We evaluated the ability of the cDNA-mediated Annealing, Selection, extension, and Ligation (DASL assay to provide highly multiplexed analyses of cryopreserved and formalin fixed, paraffin embedded (FFPE tissues obtained at autopsy. Each assay provides data on 1536 targets, and can be performed on specimens with RNA fragments as small as 60 bp. Results The DASL performed accurately and consistently with cryopreserved RNA obtained at autopsy as well as with RNA extracted from formalin-fixed paraffin embedded tissue that had a cryopreserved mirror image specimen with high quality RNA. In FFPE tissue where the cryopreserved mirror image specimen was of low quality the assay performed reproducibly on some but not all specimens. Conclusion The DASL assay provides reproducible results from cryopreserved specimens and many FFPE specimens obtained at autopsy. Gene expression analyses of these specimens may be especially valuable for the study of non-cancer endpoints, where surgical specimens are rarely available.

  4. Natural radioactivity investigations in soil samples obtained from phosphate hills in the Russaifa region, Jordan

    International Nuclear Information System (INIS)

    The specific activity, hazard index, and the annual effective dose of the terrestrial naturally occurring radionuclides (238U, 232Th, and 40K) were determined in soil samples obtained from 20 sites of phosphate hills in the Russaifa region using an HPGe-detector of 50% relative efficiency. The resolution is 2 keV for the 1.33 MeV 60Co. The soil activity ranged from 5.3 to 1201.1 Bq kg−1 for 238U, 2.2 to 31.2 Bq kg−1 for 232Th, and 19.4 to 288.4 Bq kg−1 for 40K. Annual effective dose was found in the range 0.03–0.69 mSv. The external hazard index ranged from 0.15 to 3.29 Bq kg−1, while the global value given in publications is 1 Bq kg−1. The average values of the radium equivalent activities were determined for all sites and they were found to be approximately 319 Bq kg−1. This value is below the limit of 370 Bq kg−1 recommended by . The average value of the radium equivalent activities for certain locations (sample numbers: 2, 3, 4, 9, 10, and 14) is above the same limit. - Highlights: ► External annual effective dose was evaluated in phosphate hills in the Russaifa region, Jordan. ► External hazard index parameter exceeds the limit recommended by . ► Results are an important alert to avoid the use of this soil in the construction of dwellings. ► Data obtained will serve as a baseline for assessing the radiation exposure.

  5. 3'-end sequencing for expression quantification (3SEQ from archival tumor samples.

    Directory of Open Access Journals (Sweden)

    Andrew H Beck

    Full Text Available Gene expression microarrays are the most widely used technique for genome-wide expression profiling. However, microarrays do not perform well on formalin fixed paraffin embedded tissue (FFPET. Consequently, microarrays cannot be effectively utilized to perform gene expression profiling on the vast majority of archival tumor samples. To address this limitation of gene expression microarrays, we designed a novel procedure (3'-end sequencing for expression quantification (3SEQ for gene expression profiling from FFPET using next-generation sequencing. We performed gene expression profiling by 3SEQ and microarray on both frozen tissue and FFPET from two soft tissue tumors (desmoid type fibromatosis (DTF and solitary fibrous tumor (SFT (total n = 23 samples, which were each profiled by at least one of the four platform-tissue preparation combinations. Analysis of 3SEQ data revealed many genes differentially expressed between the tumor types (FDR<0.01 on both the frozen tissue (approximately 9.6K genes and FFPET (approximately 8.1K genes. Analysis of microarray data from frozen tissue revealed fewer differentially expressed genes (approximately 4.64K, and analysis of microarray data on FFPET revealed very few (69 differentially expressed genes. Functional gene set analysis of 3SEQ data from both frozen tissue and FFPET identified biological pathways known to be important in DTF and SFT pathogenesis and suggested several additional candidate oncogenic pathways in these tumors. These findings demonstrate that 3SEQ is an effective technique for gene expression profiling from archival tumor samples and may facilitate significant advances in translational cancer research.

  6. Difficulties in obtaining representative samples for compliance with the Ballast Water Management Convention

    Digital Repository Service at National Institute of Oceanography (India)

    Carney, K.J.; Basurko, O.C.; Pazouki, K.; Marsham, S.; Delany, J.E.; Desai, D.V.; Anil, A.C.; Mesbahi, E.

    of Tetraselmis suecica was added to a storage tank and sampled at discharge. The factors holding period, initial cell density and sampling interval affected representativeness. Most samples underestimated cell density, and some tanks with an initial cell density...

  7. Molecular Identification of Leishmania Species Using Samples Obtained from Negative Stained Smears

    Directory of Open Access Journals (Sweden)

    MA Mohaghegh

    2013-06-01

    Full Text Available Background: Cutaneous Leishmaniasis (CL is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize Leishmania species among the negative direct smears obtained from skin ulcers sus­pected to CL by PCR method.Methods: Among 81 patients with suspicious skin lesions to CL referred to the Parasitology lab, nega­tive Giemsa stained smears were collected. DNA extraction performed by scraping stained smears, then PCR was performed.Results: Among the DNA extracted from smears, L. tropica was isolated from 9 (11.1% of the smears and L.major was not isolated from any samples.Conclusion: Direct microscopy on stained smears for diagnosis of leishmaniasis is not enough accu­rate. PCR is recommended for clinically suspected lesions with negative result of direct smear.

  8. Rapid Surface Sampling and Archival Record (RSSAR) system. Final report, October 1995--May 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-31

    This report describes the results of Phase 2 efforts to develop a Rapid Surface Sampling and Archival Record (RSSAR) System for the detection of semivolatile organic contaminants on concrete, transite, and metal surfaces. The characterization of equipment and building surfaces for the presence of contaminants as part of building decontamination and decommissioning activities is an immensely large task of concern to both government and industry. Because of the high cost of hazardous waste disposal, old, contaminated buildings cannot simply be demolished and scrapped. Contaminated and clean materials must be clearly identified and segregated so that the clean material can be recycled or reused, if possible, or disposed of more cheaply as nonhazardous waste. DOE has a number of sites requiring surface characterization. These sites are large, contain very heterogeneous patterns of contamination (requiring high sampling density), and will thus necessitate an enormous number of samples to be taken and analyzed. Characterization of building and equipment surfaces will be needed during initial investigations, during cleanup operations, and during the final confirmation process, increasing the total number of samples well beyond that needed for initial characterization. This multiplicity of information places a premium on the ability to handle and track data as efficiently as possible.

  9. Rapid Surface Sampling and Archival Record (RSSAR) system. Final report, October 1995 - May 1997

    International Nuclear Information System (INIS)

    This report describes the results of Phase 2 efforts to develop a Rapid Surface Sampling and Archival Record (RSSAR) System for the detection of semivolatile organic contaminants on concrete, transite, and metal surfaces. The characterization of equipment and building surfaces for the presence of contaminants as part of building decontamination and decommissioning activities is an immensely large task of concern to both government and industry. Because of the high cost of hazardous waste disposal, old, contaminated buildings cannot simply be demolished and scrapped. Contaminated and clean materials must be clearly identified and segregated so that the clean material can be recycled or reused, if possible, or disposed of more cheaply as nonhazardous waste. DOE has a number of sites requiring surface characterization. These sites are large, contain very heterogeneous patterns of contamination (requiring high sampling density), and will thus necessitate an enormous number of samples to be taken and analyzed. Characterization of building and equipment surfaces will be needed during initial investigations, during cleanup operations, and during the final confirmation process, increasing the total number of samples well beyond that needed for initial characterization. This multiplicity of information places a premium on the ability to handle and track data as efficiently as possible

  10. Characteristics of STP Pre-2004 Archived KE Basin Sludge Samples Before and After Re-Jarring in the RPL - April 2012

    Energy Technology Data Exchange (ETDEWEB)

    Sinkov, Sergey I.; Delegard, Calvin H.; Schmidt, Andrew J.; Chenault, Jeffrey W.

    2012-09-28

    This report describes results of work performed in the Shielded Analytical Laboratory (SAL) at the Pacific Northwest National Laboratory’s (PNNL) Radiochemical Processing Laboratory (RPL) with archive K East (KE) Basin sludge samples obtained before the year 2004, with some of them composited and initially characterized five years ago (Delegard et al. 2011). The previously performed testing included the physical properties determinations for selected samples (settled and particle densities, water and solids concentrations), the pH, as well as identification of crystalline phases by X-ray diffractometry (XRD) for selected samples. Another objective of the previous characterization and testing campaign was to transfer some sludge composites and individual samples into new storage containers to overcome the embrittlement effect which develops in original glass containers as a result of extended exposure to high radiation fields and which increases probability of sample loss.

  11. Coupling meteorology, metal concentrations, and Pb isotopes for source attribution in archived precipitation samples.

    Science.gov (United States)

    Graney, Joseph R; Landis, Matthew S

    2013-03-15

    A technique that couples lead (Pb) isotopes and multi-element concentrations with meteorological analysis was used to assess source contributions to precipitation samples at the Bondville, Illinois USA National Trends Network (NTN) site. Precipitation samples collected over a 16month period (July 1994-October 1995) at Bondville were parsed into six unique meteorological flow regimes using a minimum variance clustering technique on back trajectory endpoints. Pb isotope ratios and multi-element concentrations were measured using high resolution inductively coupled plasma-sector field mass spectrometry (ICP-SFMS) on the archived precipitation samples. Bondville is located in central Illinois, ~250km downwind from smelters in southeast Missouri. The Mississippi Valley Type ore deposits in Missouri provided a unique multi-element and Pb isotope fingerprint for smelter emissions which could be contrasted to industrial emissions from the Chicago and Indianapolis urban areas (~125km north and east, of Bondville respectively) and regional emissions from electric utility facilities. Differences in Pb isotopes and element concentrations in precipitation corresponded to flow regime. Industrial sources from urban areas, and thorogenic Pb from coal use, could be differentiated from smelter emissions from Missouri by coupling Pb isotopes with variations in element ratios and relative mass factors. Using a three endmember mixing model based on Pb isotope ratio differences, industrial processes in urban airsheds contributed 56±19%, smelters in southeast Missouri 26±13%, and coal combustion 18±7%, of the Pb in precipitation collected in Bondville in the mid-1990s.

  12. Test Results for Caustic Demand Measurements on Tank 241-AX-101 and Tank 241-AX-103 Archive Samples

    Energy Technology Data Exchange (ETDEWEB)

    Doll, Stephanie R. [Washington River Protection Solutions, Richland, WA (United States); Bolling, Stacie D. [Washington River Protection Solutions, Richland, WA (United States)

    2016-07-14

    Caustic demand testing is used to determine the necessary amount of caustic required to neutralize species present in the Hanford tank waste and obtain a target molarity of free hydroxide for tank corrosion control. The presence and quantity of hydroxide-consuming analytes are just as important in determining the caustic demand as is the amount of free hydroxide present. No single data point can accurately predict whether a satisfactory hydroxide level is being met as it is dependent on multiple factors (e.g., free hydroxide, buffers, amphoteric metal hydroxides, bicarbonate, etc.). This enclosure contains the caustic demand, scanning electron microscopy (SEM), polarized light microscopy (PLM), and X-ray diffraction (XRD) analysis for the tank 241-AX-101 (AX-101) and 241-AX-103 (AX-103) samples. The work was completed to fulfill a customer request outlined in the test plan, WRPS-1505529, “Test Plan and Procedure for Caustic Demand Testing on Tank 241-AX-101 and Tank 241-AX-103 Archive Samples.” The work results will provide a baseline to support planned retrieval of AX-101 and AX-103.

  13. In-depth investigation of archival and prospectively collected samples reveals no evidence for XMRV infection in prostate cancer.

    Directory of Open Access Journals (Sweden)

    Deanna Lee

    Full Text Available XMRV, or xenotropic murine leukemia virus (MLV-related virus, is a novel gammaretrovirus originally identified in studies that analyzed tissue from prostate cancer patients in 2006 and blood from patients with chronic fatigue syndrome (CFS in 2009. However, a large number of subsequent studies failed to confirm a link between XMRV infection and CFS or prostate cancer. On the contrary, recent evidence indicates that XMRV is a contaminant originating from the recombination of two mouse endogenous retroviruses during passaging of a prostate tumor xenograft (CWR22 in mice, generating laboratory-derived cell lines that are XMRV-infected. To confirm or refute an association between XMRV and prostate cancer, we analyzed prostate cancer tissues and plasma from a prospectively collected cohort of 39 patients as well as archival RNA and prostate tissue from the original 2006 study. Despite comprehensive microarray, PCR, FISH, and serological testing, XMRV was not detected in any of the newly collected samples or in archival tissue, although archival RNA remained XMRV-positive. Notably, archival VP62 prostate tissue, from which the prototype XMRV strain was derived, tested negative for XMRV on re-analysis. Analysis of viral genomic and human mitochondrial sequences revealed that all previously characterized XMRV strains are identical and that the archival RNA had been contaminated by an XMRV-infected laboratory cell line. These findings reveal no association between XMRV and prostate cancer, and underscore the conclusion that XMRV is not a naturally acquired human infection.

  14. Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

    OpenAIRE

    Ruhnke, Isabelle; DeBiasio, John V.; Suchodolski, Jan S.; Newman, Shelley; Musch, Mark W.; Steiner, Jörg M.

    2014-01-01

    This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained fr...

  15. Museums and disease: using tissue archive and museum samples to study pathogens.

    Science.gov (United States)

    Tsangaras, Kyriakos; Greenwood, Alex D

    2012-01-20

    Molecular studies of archival and fossil samples have traditionally focused on the nucleic acids derived from the host species. However, there has recently been an increase in ancient DNA research on the identification and characterization of infectious agents within the hosts. The study of pathogens from the past provides great opportunities for discovering the causes of historical infection events, characterizing host-microorganism co-evolution and directly investigating the evolution of specific pathogens. Several research teams have been able to isolate and characterize a variety of different bacterial, parasite and viral microorganisms. However, this emerging field is not without obstacles. The diagenetic processes that make ancient DNA research generally difficult are also impediments to ancient pathogen research and perhaps more so given that their DNA may represent an even rarer proportion of the remaining nucleic acids in a fossil sample than host DNA. However, studies performed under controlled conditions and following stringent ancient DNA protocols can and have yielded reliable and often surprising results. This article reviews the advantages, problems, and failures of ancient microbiological research.

  16. System for obtaining high-integrity borehole fluid samples. National Uranium Resource Evaluation

    International Nuclear Information System (INIS)

    A high-integrity borehole fluid sampling system has been developed by Bendix Field Engineering Corporation, prime contractor for the US Department of Energy Grand Junction Area Office as a part of the National Uranium Resource Evaluation program. Through the use of a high-pressure positive-displacement pump of stainless steel coupled to a Teflon delivery line, the system is designed for delivery of an uncontaminated and unfractionated fluid sample to the surface from depths of up to 300 meters. Inflatable packers may be used to isolate a zone of interest from which the representative sample is subsequently pumped. Specially designed sample receivers are used for storage of the sample under conditions comparable to downhole pressure conditions. In contrast to systems using conventional packer systems developed primarily for the oil industry, this system is capable of sealing uncased boreholes having large borehole caliper variations without the necessity of withdrawing the tool to change the packers. In addition, this new system does not introduce foreign material into the borehole since the system uses only resident borehole fluids to effect packer inflation. Thus, long-term sampling programs may be accomplished without compromising the integrity of subsequent sample acquisition

  17. Hair of the dog: obtaining samples from coyotes and wolves noninvasively

    Science.gov (United States)

    Ausband, David E.; Young, Julie; Fannin, Barbara; Mitchell, Michael S.; Stenglein, Jennifer L.; Waits, Lisette P.; Shivik, John A.

    2011-01-01

    Canids can be difficult to detect and their populations difficult to monitor. We tested whether hair samples could be collected from coyotes (Canis latrans) in Texas, USA and gray wolves (C. lupus) in Montana, USA using lure to elicit rubbing behavior at both man-made and natural collection devices. We used mitochondrial and nuclear DNA to determine whether collected hair samples were from coyote, wolf, or nontarget species. Both coyotes and wolves rubbed on man-made barbed surfaces but coyotes in Texas seldom rubbed on hanging barbed surfaces. Wolves in Montana showed a tendency to rub at stations where natural-material collection devices (sticks and debris) were present. Time to detection was relatively short (5 nights and 4 nights for coyotes and wolves, respectively) with nontarget and unknown species comprising approximately 26% of the detections in both locations. Eliciting rubbing behavior from coyotes and wolves using lures has advantages over opportunistic genetic sampling methods (e.g., scat transects) because it elicits a behavior that deposits a hair sample at a fixed sampling location, thereby increasing the efficiency of sampling for these canids. Hair samples from rub stations could be used to provide estimates of abundance, measures of genetic diversity and health, and detection-nondetection data useful for cost-effective population monitoring.

  18. Global distribution of polymorphisms associated with delayed Plasmodium falciparum parasite clearance following artemisinin treatment: genotyping of archive blood samples.

    Science.gov (United States)

    Murai, Kenji; Culleton, Richard; Hisaoka, Teruhiko; Endo, Hiroyoshi; Mita, Toshihiro

    2015-06-01

    The recent emergence and spread of artemisinin-resistant Plasmodium falciparum isolates is a growing concern for global malaria-control efforts. A recent genome-wide analysis study identified two SNPs at genomic positions MAL10-688956 and MAL13-1718319, which are linked to delayed clearance of parasites following artemisinin combination therapy (ACT). It is expected that continuous artemisinin pressure will affect the distribution of these SNPs. Here, we investigate the worldwide distribution of these SNPs using a large number of archived samples in order to generate baseline data from the period before the emergence of ACT resistance. The presence of SNPs in MAL10-688956 and MAL13-1718319 was assessed by nested PCR RFLP and direct DNA sequencing using 653 global P. falciparum samples obtained before the reported emergence of ACT resistance. SNPs at MAL10-688956 and MAL13-1718319 associated with delayed parasite clearance following ACT administration were observed in 8% and 3% of parasites, respectively, mostly in Cambodia and Thailand. Parasites harbouring both SNPs were found in only eight (1%) isolates, all of which were from Cambodia and Thailand. Linkage disequilibrium was detected between MAL10-688956 and MAL13-1718319, suggesting that this SNP combination may have been selected by ACT drug pressure. Neither of the SNPs associated with delayed parasite clearance were observed in samples from Africa or South America. Baseline information of the geographical difference of MAL10-688956 and MAL13-1718319 SNPs provides a solid basis for assessing whether these SNPs are selected by artemisinin-based combination therapies.

  19. A noninvasive hair sampling technique to obtain high quality DNA from elusive small mammals.

    Science.gov (United States)

    Henry, Philippe; Henry, Alison; Russello, Michael A

    2011-03-13

    Noninvasive genetic sampling approaches are becoming increasingly important to study wildlife populations. A number of studies have reported using noninvasive sampling techniques to investigate population genetics and demography of wild populations. This approach has proven to be especially useful when dealing with rare or elusive species. While a number of these methods have been developed to sample hair, feces and other biological material from carnivores and medium-sized mammals, they have largely remained untested in elusive small mammals. In this video, we present a novel, inexpensive and noninvasive hair snare targeted at an elusive small mammal, the American pika (Ochotona princeps). We describe the general set-up of the hair snare, which consists of strips of packing tape arranged in a web-like fashion and placed along travelling routes in the pikas' habitat. We illustrate the efficiency of the snare at collecting a large quantity of hair that can then be collected and brought back to the lab. We then demonstrate the use of the DNA IQ system (Promega) to isolate DNA and showcase the utility of this method to amplify commonly used molecular markers including nuclear microsatellites, amplified fragment length polymorphisms (AFLPs), mitochondrial sequences (800bp) as well as a molecular sexing marker. Overall, we demonstrate the utility of this novel noninvasive hair snare as a sampling technique for wildlife population biologists. We anticipate that this approach will be applicable to a variety of small mammals, opening up areas of investigation within natural populations, while minimizing impact to study organisms.

  20. Analyses of human colonic mucus obtained by an in vivo sampling technique

    NARCIS (Netherlands)

    Hamer, H.M.; Jonkers, D.M.A.E.; Loof, A.; Houtvin, S.A.L.W. van; Troost, F.J.; Venema, K.; Kodde, A.; Koek, G.H.; Schipper, R.G.; Heerde, W.L. van; Brummer, R.J.

    2009-01-01

    Background: The mucus layer is an important dynamic component of the epithelial barrier. It contains mucin glycoproteins and other compounds secreted by the intestinal epithelium, such as secretory IgA. However, a standardized in vivo sampling technique of mucus in humans is not yet available. Aim:

  1. Molecular detection of Streptococcus agalactiae in bovine raw milk samples obtained directly from bulk tanks.

    Science.gov (United States)

    Elias, Acácia Orieth; Cortez, Adriana; Brandão, Paulo Eduardo; da Silva, Rodrigo Costa; Langoni, Helio

    2012-08-01

    Mastitis is the most common infectious disease affecting dairy cattle; in addition, it remains the most economically important disease of dairy industries around the world. Streptococcus agalactiae, a contagious pathogen associated with subclinical mastitis, is highly infectious. This bacterium can cause an increase in bulk tank bacterial counts (BTBC) and bulk tank somatic cell counts (BTSCC). The microbiological identification of S. agalactiae in samples from bulk tanks is an auxiliary method to control contagious mastitis. Thus, there are some limitations for time-consuming cultures or identification methods and additional concerns about the conservation and transport of samples. Bulk tank samples from 247 dairy farms were cultured and compared through polymerase chain reaction (PCR), directed to 16S rRNA genes of S. agalactiae, followed by BTBC and S. agalactiae isolation. The mean value of BTBC was 1.08×10(6) CFU mL(-1) and the bacterium was identified through the microbiological method in 98 (39.7%; CI(95%)=33.8-45.9%) and through PCR in 110 (44.5%; CI(95%)=38.5-50.8%) samples. Results indicated sensitivity of 0.8571±0.0353 (CI(95%)=0.7719-0.9196) and specificity of 0.8255±0.0311 (CI(95%)=0.7549-0.8827). The lack of significant difference between microbiological and molecular results (κ=0.6686±0.0477 and CI(95%)=0.5752-0.7620) indicated substantial agreement between the methods. This suggests that PCR can be used for bulk tank samples to detect contagious mastitis caused by S. agalactiae.

  2. Thermal diffusivity and electron transport properties of NTC samples obtained by the photoacoustic method

    Energy Technology Data Exchange (ETDEWEB)

    Savic, S.M. [Institute of Technical Sciences of SASA, Knez Mihailova 35/IV, 11000 Belgrade (Serbia); Aleksic, O.S. [Center for Multidisciplinary Studies of the University of Belgrade, Kneza Viseslava 1, 11000 Belgrade (Serbia); Nikolic, M.V. [Center for Multidisciplinary Studies of the University of Belgrade, Kneza Viseslava 1, 11000 Belgrade (Serbia); Lukovic, D.T. [Institute of Technical Sciences of SASA, Knez Mihailova 35/IV, 11000 Belgrade (Serbia); Pejovic, V.Z. [Center for Multidisciplinary Studies of the University of Belgrade, Kneza Viseslava 1, 11000 Belgrade (Serbia); Nikolic, P.M. [Institute of Technical Sciences of SASA, Knez Mihailova 35/IV, 11000 Belgrade (Serbia)]. E-mail: nikolic@sanu.ac.yu

    2006-07-15

    Thermal diffusivity and electron transport parameters of sintered NTC samples were determined by the photoacoustic (PA) technique. Powder mixtures composed of MnO, NiO, CoO and Fe{sub 2}O{sub 3} were milled to nanometer particle size. NTC discs were dry powder pressed and sintered at different temperatures in the range from 900 deg. C to 1300 deg. C for 30 min. A second group of NTC discs was sintered at 1200 deg. C with the sintering time varying from 30 min to 360 min. These NTC samples were polished and exposed to a chopped laser beam in order to plot a response in the acoustic range. The thermal diffusivity of sintered NTC layers based on a metal oxide powder mixture was measured at room temperature by the photoacoustic technique. An increase of thermal diffusivity with the sintering temperature and time of sintering was observed.

  3. Pumping time required to obtain tube well water samples with aquifer characteristic radon concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Ricardo, Carla Pereira; Oliveira, Arno Heeren de, E-mail: heeren@nuclear.ufmg.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Escola de Engenharia. Dept. de Engenharia Nuclear; Rocha, Zildete; Palmieri, Helena E.L.; Linhares, Maria G.M.; Menezes, Maria Angela B.C., E-mail: rochaz@cdtn.br, E-mail: help@cdtn.br, E-mail: mgml@cdtn.br, E-mail: menezes@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2011-07-01

    Radon is an inert noble gas, which comes from the natural radioactive decay of uranium and thorium in soil, rock and water. Radon isotopes emanated from radium-bearing grains of a rock or soil are released into the pore space. Radon that reaches the pore space is partitioned between the gaseous and aqueous phases. Thus, the groundwater presents a radon signature from the rock that is characteristic of the aquifer. The characteristic radon concentration of an aquifer, which is mainly related to the emanation, is also influenced by the degree of subsurface degassing, especially in the vicinity of a tube well, where the radon concentration is strongly reduced. Looking for the required pumping time to take a tube well water sample that presents the characteristic radon concentration of the aquifer, an experiment was conducted in an 80 m deep tube well. In this experiment, after twenty-four hours without extraction, water samples were collected periodically, about ten minutes intervals, during two hours of pumping time. The radon concentrations of the samples were determined by using the RAD7 Electronic Radon Detector from Durridge Company, a solid state alpha spectrometric detector. It was realized that the necessary time to reach the maximum radon concentration, that means the characteristic radon concentration of the aquifer, is about sixty minutes. (author)

  4. Analysis of the Magnetocaloric Effect in Powder Samples Obtained by Ball Milling

    Science.gov (United States)

    Blázquez, J. S.; Ipus, J. J.; Moreno-Ramírez, L. M.; Borrego, J. M.; Lozano-Pérez, S.; Franco, V.; Conde, C. F.; Conde, A.

    2015-06-01

    Since the discovery of the giant magnetocaloric effect (MCE) close to room temperature in FeRh and particularly in Gd5Si2Ge2 compounds, the study of this phenomenon has experienced an exponential growth. Among the different techniques used to produce magnetocaloric materials, ball milling has been shown as a very versatile one and presents several advantages over other preparation techniques ( e.g., easy scale-up to industrial production). Although a general decrease of the peak value of the magnetic entropy change is observed for milled samples, it can be compensated by the large broadening of the MCE peak, leading to an increase of the refrigeration capacity. In this short review, several aspects inherent to powder samples affecting MCE will be discussed, such as the relevant effect of the demagnetizing field, the possible multiphase character, and the presence of Curie temperature distributions. In mechanically alloyed samples, the two latter factors are typically affected by the degree of integration of the different starting constituents.

  5. Deparaffinization with mineral oil: a simple procedure for extraction of high-quality DNA from archival formalin-fixed paraffin-embedded samples.

    Science.gov (United States)

    Heikal, Nahla; Nussenzveig, Roberto H; Agarwal, Archana M

    2014-09-01

    Extracting DNA from formalin-fixed paraffin-embedded (FFPE) archival samples remains difficult. Successful polymerase chain reactions (PCR) with DNA extracted from FFPE samples is still very low. We extracted DNA from 12 recent and old archival FFPE bone marrow trephine biopsies by use of a simple protocol on the basis of deparaffinization with molecular biology-grade mineral oil followed by DNA extraction with the Qiagen FFPE kit. Comparison of this deparaffinization method with standard protocols, for example, xylene or Hemo-D with subsequent rehydration using graded ethanols, was investigated. The quality and quantity of extracted DNA were tested by a combination of ultraviolet spectroscopy, analysis on a Caliper LabChip GX, and real-time PCR combined with high-resolution melt analysis. Highest quality PCR-amplifiable DNA was obtained by deparaffinization with mineral oil, whereas more variable results were obtained for the other 2 deparaffinization procedures. This result was confirmed by real-time PCR and high-resolution melt analysis. Besides improvements in the quality of extracted DNA, use of mineral oil for deparaffinization has the added benefit of decreased time (20 vs. 75 min) and a significant reduction of hands-on labor (1 step vs. multiple hands-on centrifugation and decanting steps).

  6. Bacterial populations in samples of bioleached copper ore as revealed by analysis of DNA obtained before and after cultivation.

    OpenAIRE

    Pizarro, J.; Jedlicki, E; Orellana, O; J. Romero; Espejo, R T

    1996-01-01

    The composition of bacterial populations in copper bioleaching systems was investigated by analysis of DNA obtained either directly from ores or leaching solutions or after laboratory cultures. This analysis consisted of the characterization of the spacer regions between the 16 and 23S genes in the bacterial rRNA genetic loci after PCR amplification. The sizes of the spacer regions, amplified from DNAs obtained from samples, were compared with the sizes of those obtained from cultures of the ...

  7. Atypical carcinoid and large cell neuroendocrine carcinoma of the lung: a proteomic dataset from formalin-fixed archival samples

    Science.gov (United States)

    Tanca, Alessandro; Addis, Maria Filippa; Pisanu, Salvatore; Abbondio, Marcello; Pagnozzi, Daniela; Eccher, Albino; Rindi, Guido; Cossu-Rocca, Paolo; Uzzau, Sergio; Fanciulli, Giuseppe

    2016-01-01

    Here we present a dataset generated using formalin-fixed paraffin-embedded archival samples from two rare lung neuroendocrine tumor subtypes (namely, two atypical carcinoids, ACs, and two large-cell neuroendocrine carcinomas, LCNECs). Samples were subjected to a shotgun proteomics pipeline, comprising full-length protein extraction, SDS removal through spin columns, in solution trypsin digestion, long gradient liquid chromatography peptide separation and LTQ-Orbitrap mass spectrometry analysis. A total of 1260 and 2436 proteins were identified in the AC and LCNEC samples, respectively, with FDR <1%. MS data are available in the PeptideAtlas repository at http://www.peptideatlas.org/PASS/PASS00375. PMID:27054153

  8. Atypical carcinoid and large cell neuroendocrine carcinoma of the lung: a proteomic dataset from formalin-fixed archival samples.

    Science.gov (United States)

    Tanca, Alessandro; Addis, Maria Filippa; Pisanu, Salvatore; Abbondio, Marcello; Pagnozzi, Daniela; Eccher, Albino; Rindi, Guido; Cossu-Rocca, Paolo; Uzzau, Sergio; Fanciulli, Giuseppe

    2016-06-01

    Here we present a dataset generated using formalin-fixed paraffin-embedded archival samples from two rare lung neuroendocrine tumor subtypes (namely, two atypical carcinoids, ACs, and two large-cell neuroendocrine carcinomas, LCNECs). Samples were subjected to a shotgun proteomics pipeline, comprising full-length protein extraction, SDS removal through spin columns, in solution trypsin digestion, long gradient liquid chromatography peptide separation and LTQ-Orbitrap mass spectrometry analysis. A total of 1260 and 2436 proteins were identified in the AC and LCNEC samples, respectively, with FDR <1%. MS data are available in the PeptideAtlas repository at http://www.peptideatlas.org/PASS/PASS00375.

  9. Atypical carcinoid and large cell neuroendocrine carcinoma of the lung: a proteomic dataset from formalin-fixed archival samples

    Directory of Open Access Journals (Sweden)

    Alessandro Tanca

    2016-06-01

    Full Text Available Here we present a dataset generated using formalin-fixed paraffin-embedded archival samples from two rare lung neuroendocrine tumor subtypes (namely, two atypical carcinoids, ACs, and two large-cell neuroendocrine carcinomas, LCNECs. Samples were subjected to a shotgun proteomics pipeline, comprising full-length protein extraction, SDS removal through spin columns, in solution trypsin digestion, long gradient liquid chromatography peptide separation and LTQ-Orbitrap mass spectrometry analysis. A total of 1260 and 2436 proteins were identified in the AC and LCNEC samples, respectively, with FDR <1%. MS data are available in the PeptideAtlas repository at http://www.peptideatlas.org/PASS/PASS00375.

  10. A Method to Correlate mRNA Expression Datasets Obtained from Fresh Frozen and Formalin-Fixed, Paraffin-Embedded Tissue Samples: A Matter of Thresholds.

    Directory of Open Access Journals (Sweden)

    Dana A M Mustafa

    Full Text Available Gene expression profiling of tumors is a successful tool for the discovery of new cancer biomarkers and potential targets for the development of new therapeutic strategies. Reliable profiling is preferably performed on fresh frozen (FF tissues in which the quality of nucleic acids is better preserved than in formalin-fixed paraffin-embedded (FFPE material. However, since snap-freezing of biopsy materials is often not part of daily routine in pathology laboratories, one may have to rely on archival FFPE material. Procedures to retrieve the RNAs from FFPE materials have been developed and therefore, datasets obtained from FFPE and FF materials need to be made compatible to ensure reliable comparisons are possible.To develop an efficient method to compare gene expression profiles obtained from FFPE and FF samples using the same platform.Twenty-six FFPE-FF sample pairs of the same tumors representing various cancer types, and two FFPE-FF sample pairs of breast cancer cell lines, were included. Total RNA was extracted and gene expression profiling was carried out using Illumina's Whole-Genome cDNA-mediated Annealing, Selection, extension and Ligation (WG-DASL V3 arrays, enabling the simultaneous detection of 24,526 mRNA transcripts. A sample exclusion criterion was created based on the expression of 11 stably expressed reference genes. Pearson correlation at the probe level was calculated for paired FFPE-FF, and three cut-off values were chosen. Spearman correlation coefficients between the matched FFPE and FF samples were calculated for three probe lists with varying levels of significance and compared to the correlation based on all measured probes. Unsupervised hierarchical cluster analysis was performed to verify performance of the included probe lists to compare matched FPPE-FF samples.Twenty-seven FFPE-FF pairs passed the sample exclusion criterion. From the profiles of 27 FFPE and FF matched samples, the best correlating probes were identified

  11. Genotypic characterization of Staphylococcus aureus obtained from humans and bovine mastitis samples in India

    Directory of Open Access Journals (Sweden)

    K Prashanth

    2011-01-01

    Full Text Available Aim and Background: Staphylococcus aureus is a major human pathogen that also causes important infections in cattle and sheep. The present study aimed to test genetic diversity among strains of S. aureus isolated from cattle (n=34 and humans (n=22 by DNA typing. Materials and Methods: Fluorescent amplified fragment length polymorphism (FAFLP is the genotyping tool used in the study. The presence of the mecA and Panton-Valentine leukocidin (PVL genes among these strain groups was also checked. Results: A dendrogram deduced from FAFLP showed that all the strains clustered into 10 groups (A-J with a relative genetic divergence of less than 8%. Sixty-seven percent of the isolates from bovine sources clustered together in two clades (A and H, while another major cluster with 13 isolates (59% (Cluster G had all strains from a human host. The remaining strains from both the hosts clustered independently into smaller clusters with the exception of two strains of human origin, which clustered along with a bovine cluster. Thirteen strains belonging to cluster G were highly clonal. About 77% of strains obtained from human infections were methicillin-resistant S. aureus (MRSA, whereas only 29% of strains from bovine origin were MRSA. Only three strains from human origin showed PVL positive, while no strain from cattle had PVL genes. The complete absence of PVL genes in all the bovine strains in the study appears to be significant. Conclusions: FAFLP can be successfully applied to assess the genetic relationship of S. aureus isolates from different hosts. The study also provided the valuable epidemiological data on S. aureus from bovine sources in India, which is lacking.

  12. Design of a protocol for obtaining genomic DNA from saliva using mouthwash: Samples taken from patients with periodontal disease

    Science.gov (United States)

    Mendoza, Ángel Chávez; Volante, Beatriz Buentello; Hernández, María Esther Ocharán; Mendoza, Claudia Camelia Calzada; Pliego, Arturo Flores; Baptista Gonzalez, Héctor A.; Juárez, Higinio Estrada

    2016-01-01

    Background Obtaining high quality genomic DNA safely and economically is vital for diverse studies of large populations aimed at evaluating the role of genetic factors in susceptibility to disease. Aim This study was to test a protocol for the extraction of high quality genomic DNA from saliva samples obtained with mouthwash and taken from patients with periodontal disease. Methods Saliva samples were taken from 60 patients and then stored at room temperature. DNA extraction was carried out at distinct post-sampling times (10, 20 and 30 days). Evaluation of genomic DNA was performed with spectrophotometry, electrophoresis, and PCR genotyping and sequencing. Results The greatest concentration of DNA obtained was 352 μg at 10 days post-sampling, followed by 121.025 μg and 19.59 μg at 20 and 30 days, respectively. When determining the purity of DNA with the spectrophotometric ratio of 260/230, the relations of 1.20, 1.40 and 0.781 were obtained for 10, 20 and 30 days, respectively. In all samples, it was possible to amplify the product of 485 bp and the sequence of the amplicons showed 95% similarity to the reference sequence. Conclusion The present protocol represents an easy, safe and economical technique for obtaining high quality genomic DNA. PMID:27195211

  13. Evidence for obtaining a second successive semen sample for intrauterine insemination in selected patients: results from 32 consecutive cases

    Science.gov (United States)

    Ortiz, Alejandra; Ortiz, Rita; Soto, Evelyn; Hartmann, Jonathan; Manzur, Alejandro

    2016-01-01

    Objective The goal of this study was to compare the semen parameters of two successive samples obtained within an interval of less than 60 minutes from patients planning to undergo intrauterine insemination (IUI) whose first samples exhibited low semen quality. Methods Thirty-two consecutive patients were enrolled in the study. On the day of IUI, the semen analysis of the samples initially presented by all patients met at least two of the following criteria: sperm concentration <5×106/mL, total sperm count <10×106, progressive sperm motility (a+b) in the native sample <30%, and total motile sperm count (TMSC) <4×106. A successive semen sample was obtained no more than 60 minutes after the first sample. Results Compared to the first sample, the second exhibited significantly (p<0.05) improved sperm concentration, TMSC, progressive motility, and vitality. Regarding TMSC, the most critical parameter on the day of IUI, 23 patients (71.8%) improved it, while nine (28.2%) displayed poorer outcomes. Conclusion In defined cases, requesting a second successive ejaculate on the day of insemination may result in a high percentage of cases in an improvement of the quality of the sample. PMID:27358828

  14. miRNA Expression Profiling Enables Risk Stratification in Archived and Fresh Neuroblastoma Tumor Samples

    NARCIS (Netherlands)

    K. de Preter; P. Mestdagh; J. Vermeulen; F. Zeka; A. Naranjo; I. Bray; V. Castel; C. Chen; E. Drozynska; A. Eggert; M.D. Hogarty; E. Izycka-Swieszewska; W.B. London; R. Noguera; M. Piqueras; K. Bryan; B. Schowe; P. van Sluis; J.J. Molenaar; A Schramm; J.H. Schulte; R.L. Stallings; R. Versteeg; G. Laureys; N. van Roy; F. Speleman; J. Vandesompele

    2011-01-01

    Purpose: More accurate assessment of prognosis is important to further improve the choice of risk-related therapy in neuroblastoma (NB) patients. In this study, we aimed to establish and validate a prognostic miRNA signature for children with NB and tested it in both fresh frozen and archived formal

  15. Evaluation of Rock Powdering Methods to Obtain Fine-grained Samples for CHEMIN, a Combined XRD/XRF Instrument

    Science.gov (United States)

    Chipera, S. J.; Vaniman, D. T.; Bish, D. L.; Sarrazin, P.; Feldman, S.; Blake, D. F.; Bearman, G.; Bar-Cohen, Y.

    2004-01-01

    A miniature XRD/XRF (X-ray diffraction / X-ray fluorescence) instrument, CHEMIN, is currently being developed for definitive mineralogic analysis of soils and rocks on Mars. One of the technical issues that must be addressed to enable remote XRD analysis is how best to obtain a representative sample powder for analysis. For powder XRD analyses, it is beneficial to have a fine-grained sample to reduce preferred orientation effects and to provide a statistically significant number of crystallites to the X-ray beam. Although a two-dimensional detector as used in the CHEMIN instrument will produce good results even with poorly prepared powder, the quality of the data will improve and the time required for data collection will be reduced if the sample is fine-grained and randomly oriented. A variety of methods have been proposed for XRD sample preparation. Chipera et al. presented grain size distributions and XRD results from powders generated with an Ultrasonic/Sonic Driller/Corer (USDC) currently being developed at JPL. The USDC was shown to be an effective instrument for sampling rock to produce powder suitable for XRD. In this paper, we compare powder prepared using the USDC with powder obtained with a miniaturized rock crusher developed at JPL and with powder obtained with a rotary tungsten carbide bit to powders obtained from a laboratory bench-scale Retsch mill (provides benchmark mineralogical data). These comparisons will allow assessment of the suitability of these methods for analysis by an XRD/XRF instrument such as CHEMIN.

  16. Effect of aggregation of horn fly populations within cattle herds and consequences for sampling to obtain unbiased estimates of abundance.

    Science.gov (United States)

    Lysyk, T J; Steelman, C D

    2004-07-01

    Reanalysis of counts of horn fly, Hematobia irritans (L.), obtained from a variety of cattle herds indicated that aggregation of the flies within herds decreased as mean fly density increased. Aggregation was also related to the proportion of fly-resistant and fly-susceptible cattle in a herd. Herds were grouped according to their degree of horn fly aggregation. Low aggregation herds included larger framed Angus, Horned Hereford, Polled Hereford, and Red Poll breeds. Moderate aggregation occurred with Brahman, Charolais, small-framed Angus, mixed cows, and Hereford x Charolais cross. High aggregation occurred with Chianina and mixed herds. Relationships between the sample means and variances varied among aggregation groups. A resampling approach was used to determine the influence of random sampling of a herd on the proportion of horn fly population estimates within fixed percentages of the true mean. The proportion of sample means within +/- 5, 10, 15, and 20% of the true means varied with the proportion of the herd sampled, the mean and variance of fly density, and herd size. Recommendations for obtaining sample size to estimate fly density within a fixed percentage of the true mean are given. PMID:15311450

  17. Utility of the microculture method in non-invasive samples obtained from an experimental murine model with asymptomatic leishmaniasis.

    Science.gov (United States)

    Allahverdiyev, Adil M; Bagirova, Malahat; Cakir-Koc, Rabia; Elcicek, Serhat; Oztel, Olga Nehir; Canim-Ates, Sezen; Abamor, Emrah Sefik; Yesilkir-Baydar, Serap

    2012-07-01

    The sensitivity of diagnostic methods for visceral leishmaniasis (VL) decreases because of the low number of parasites and antibody amounts in asymptomatic healthy donors who are not suitable for invasive sample acquisition procedures. Therefore, new studies are urgently needed to improve the sensitivity and specificity of the diagnostic approaches in non-invasive samples. In this study, the sensitivity of the microculture method (MCM) was compared with polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and immunofluorescent antibody test (IFAT) methods in an experimental murine model with asymptomatic leishmaniasis. Results showed that the percent of positive samples in ELISA, IFAT, and peripheral blood (PB) -PCR tests were 17.64%, 8.82%, and 5.88%, respectively, whereas 100% positive results were obtained with MCM and MCM-PCR methods. Thus, this study, for the first time, showed that MCM is more sensitive, specific, and economic than other methods, and the sensitivity of PCR that was performed to samples obtained from MCM was higher than sensitivity of the PCR method sampled by PB. PMID:22764296

  18. Utility of the microculture method for Leishmania detection in non-invasive samples obtained from a blood bank.

    Science.gov (United States)

    Ates, Sezen Canim; Bagirova, Malahat; Allahverdiyev, Adil M; Kocazeybek, Bekir; Kosan, Erdogan

    2013-10-01

    In recent years, the role of donor blood has taken an important place in epidemiology of Leishmaniasis. According to the WHO, the numbers of patients considered as symptomatic are only 5-20% of individuals with asymptomatic leishmaniasis. In this study for detection of Leishmania infection in donor blood samples, 343 samples from the Capa Red Crescent Blood Center were obtained and primarily analyzed by microscopic and serological methods. Subsequently, the traditional culture (NNN), Immuno-chromatographic test (ICT) and Polymerase Chain Reaction (PCR) methods were applied to 21 samples which of them were found positive with at least one method. Buffy coat (BC) samples from 343 blood donors were analyzed: 15 (4.3%) were positive by a microculture method (MCM); and 4 (1.1%) by smear. The sera of these 343 samples included 9 (2.6%) determined positive by ELISA and 7 (2%) positive by IFAT. Thus, 21 of (6.1%) the 343 subjects studied by smear, MCM, IFAT and ELISA techniques were identified as positive for leishmaniasis at least one of the techniques and the sensitivity assessed. According to our data, the sensitivity of the methods are identified as MCM (71%), smear (19%), IFAT (33%), ELISA (42%), NNN (4%), PCR (14%) and ICT (4%). Thus, with this study for the first time, the sensitivity of a MCM was examined in blood donors by comparing MCM with the methods used in the diagnosis of leishmaniasis. As a result, MCM was found the most sensitive method for detection of Leishmania parasites in samples obtained from a blood bank. In addition, the presence of Leishmania parasites was detected in donor bloods in Istanbul, a non-endemic region of Turkey, and these results is a vital importance for the health of blood recipients.

  19. Utility of the microculture method for Leishmania detection in non-invasive samples obtained from a blood bank.

    Science.gov (United States)

    Ates, Sezen Canim; Bagirova, Malahat; Allahverdiyev, Adil M; Kocazeybek, Bekir; Kosan, Erdogan

    2013-10-01

    In recent years, the role of donor blood has taken an important place in epidemiology of Leishmaniasis. According to the WHO, the numbers of patients considered as symptomatic are only 5-20% of individuals with asymptomatic leishmaniasis. In this study for detection of Leishmania infection in donor blood samples, 343 samples from the Capa Red Crescent Blood Center were obtained and primarily analyzed by microscopic and serological methods. Subsequently, the traditional culture (NNN), Immuno-chromatographic test (ICT) and Polymerase Chain Reaction (PCR) methods were applied to 21 samples which of them were found positive with at least one method. Buffy coat (BC) samples from 343 blood donors were analyzed: 15 (4.3%) were positive by a microculture method (MCM); and 4 (1.1%) by smear. The sera of these 343 samples included 9 (2.6%) determined positive by ELISA and 7 (2%) positive by IFAT. Thus, 21 of (6.1%) the 343 subjects studied by smear, MCM, IFAT and ELISA techniques were identified as positive for leishmaniasis at least one of the techniques and the sensitivity assessed. According to our data, the sensitivity of the methods are identified as MCM (71%), smear (19%), IFAT (33%), ELISA (42%), NNN (4%), PCR (14%) and ICT (4%). Thus, with this study for the first time, the sensitivity of a MCM was examined in blood donors by comparing MCM with the methods used in the diagnosis of leishmaniasis. As a result, MCM was found the most sensitive method for detection of Leishmania parasites in samples obtained from a blood bank. In addition, the presence of Leishmania parasites was detected in donor bloods in Istanbul, a non-endemic region of Turkey, and these results is a vital importance for the health of blood recipients. PMID:23806567

  20. Study of anti corrosive behaviour on A I 6061 samples covered with Ni-P alloys obtained by autocatalytic method

    International Nuclear Information System (INIS)

    There are many ways to keep safe an industrial material from corrosion attack.One is covering the piece with a layer of another material which corrosion resistance is higher to the one of the element to protect.The anticorrosion protection mechanism is achieved by the formation of a physical pore less barrier without any defects.This avoid the arrival of those agents from environment responsible of electrochemical attack.In this paper, corrosion resistance of metallic coatings over nuclear usage aluminum samples is analyzed.Our interest is aimed on nickel I phosphorous alloy coatings (Ni I P) obtained by electroless method (autocatalytic) over Al 6061 alloy samples.A comparative study is carried on with different phosphorous contents but always under 12 %.This job is completed with other nickel coating, Vitro vac 0080 (with no phosphorous content) in order to compare structures and anti corrosive properties.Besides, the comparison between mentioned materials and aluminum samples is made.The study is carried on using superficial characterization of each sample with or without coating through a series of complementary techniques such as chemical, electrochemical (linear sweep voltammetry, cyclic voltammetry, polarization resistance determination) and physical (scanning electronic microscopy, hardness determination) techniques.Finally, variable correlation is made as a function of the phosphorous content in the samples used in the experiences.The coating structure obtained is amorphous.It presents no pore or failure and its hardness shows important values.The electrochemical analysis allows to check that anti corrosive protection capacity of Ni-P alloy increases with the phosphorous content in the coat. Al 6061 by itself demonstrate an electrochemically bad behaviour.Substrate I coating adherence is very good

  1. [A Large Number of Circulating Tumor Cells(CTCs)Can Be Isolated from Samples Obtained by Using Leukapheresis Procedures].

    Science.gov (United States)

    Soya, Ryoko; Taguchi, Jyunichi; Nagakawa, Yuichi; Takahashi, Osamu; Sandoh, Norimasa; Hosokawa, Yuichi; Kasuya, Kazuhiko; Umeda, Naoki; Okamoto, Masato; Tsujitani, Shunichi; Tsuchida, Akihiko

    2015-09-01

    We hypothesized that a large number of circulating tumor cells(CTCs)may be isolated from samples obtained by using the leukapheresis procedures that are utilized to collect peripheral blood mononuclear cells for dendritic cell vaccine therapy. We utilized the CellSearch System to determine the number of CTCs in samples obtained by using leukapheresis in 7 patients with colorectal cancer, 5 patients with breast cancer, and 3 patients with gastric cancer. In all patients, a large number of CTCs were isolated. The mean number of CTCs per tumor was 17.1(range 10-34)in colorectal cancer, 10.0(range 2-27)in breast cancer, and 24.0(range 2-42)in gastric cancer. We succeeded in culturing the isolated CTCs from 7 patients with colorectal cancer, 5 patients with breast cancer, and 3 patients with gastric cancer. In conclusion, compared to conventional methods, a large number of CTCs can be obtained by using leukapheresis procedures. The molecular analyses of the CTCs isolated by using this method should be promising in the development of personalized cancer treatments.

  2. The effects of age-in-block on RNA-seq analysis of archival formalin-fixed paraffin-embedded (FFPE) samples

    Science.gov (United States)

    Archival samples represent a vast resource for identification of chemical and pharmaceutical targets. Previous use of formalin-fixed paraffin-embedded (FFPE) samples has been limited due to changes in RNA introduced by fixation and embedding procedures. Recent advances in RNA-seq...

  3. Tissue Microarray Technology for Molecular Applications: Investigation of Cross-Contamination between Tissue Samples Obtained from the Same Punching Device

    Directory of Open Access Journals (Sweden)

    Erik Vassella

    2015-04-01

    Full Text Available Background: Tissue microarray (TMA technology allows rapid visualization of molecular markers by immunohistochemistry and in situ hybridization. In addition, TMA instrumentation has the potential to assist in other applications: punches taken from donor blocks can be placed directly into tubes and used for nucleic acid analysis by PCR approaches. However, the question of possible cross-contamination between samples punched with the same device has frequently been raised but never addressed. Methods: Two experiments were performed. (1 A block from mycobacterium tuberculosis (TB positive tissue and a second from an uninfected patient were aligned side-by-side in an automated tissue microarrayer. Four 0.6 mm punches were cored from each sample and placed inside their corresponding tube. Between coring of each donor block, a mechanical cleaning step was performed by insertion of the puncher into a paraffin block. This sequence of coring and cleaning was repeated three times, alternating between positive and negative blocks. A fragment from the 6110 insertion sequence specific for mycobacterium tuberculosis was analyzed; (2 Four 0.6 mm punches were cored from three KRAS mutated colorectal cancer blocks, alternating with three different wild-type tissues using the same TMA instrument (sequence of coring: G12D, WT, G12V, WT, G13D and WT. Mechanical cleaning of the device between each donor block was made. Mutation analysis by pyrosequencing was carried out. This sequence of coring was repeated manually without any cleaning step between blocks. Results/Discussion: In both analyses, all alternating samples showed the expected result (samples 1, 3 and 5: positive or mutated, samples 2, 4 and 6: negative or wild-type. Similar results were obtained without cleaning step. These findings suggest that no cross-contamination of tissue samples occurs when donor blocks are punched using the same device, however a cleaning step is nonetheless recommended. Our

  4. File list: Unc.Kid.20.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Kid.20.AllAg.Nephrectomy_sample hg19 Unclassified Kidney Nephrectomy sample htt...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.Kid.20.AllAg.Nephrectomy_sample.bed ...

  5. File list: DNS.Kid.20.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Kid.20.AllAg.Nephrectomy_sample hg19 DNase-seq Kidney Nephrectomy sample http:/.../dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.Kid.20.AllAg.Nephrectomy_sample.bed ...

  6. File list: DNS.Kid.50.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Kid.50.AllAg.Nephrectomy_sample hg19 DNase-seq Kidney Nephrectomy sample http:/.../dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.Kid.50.AllAg.Nephrectomy_sample.bed ...

  7. File list: DNS.Kid.05.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Kid.05.AllAg.Nephrectomy_sample hg19 DNase-seq Kidney Nephrectomy sample http:/.../dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.Kid.05.AllAg.Nephrectomy_sample.bed ...

  8. File list: Unc.Kid.10.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Kid.10.AllAg.Nephrectomy_sample hg19 Unclassified Kidney Nephrectomy sample htt...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.Kid.10.AllAg.Nephrectomy_sample.bed ...

  9. File list: Pol.Kid.50.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Kid.50.AllAg.Nephrectomy_sample hg19 RNA polymerase Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Kid.50.AllAg.Nephrectomy_sample.bed ...

  10. File list: Unc.Kid.50.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Kid.50.AllAg.Nephrectomy_sample hg19 Unclassified Kidney Nephrectomy sample htt...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.Kid.50.AllAg.Nephrectomy_sample.bed ...

  11. File list: Pol.Kid.05.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Kid.05.AllAg.Nephrectomy_sample hg19 RNA polymerase Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Kid.05.AllAg.Nephrectomy_sample.bed ...

  12. Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehensive database

    Directory of Open Access Journals (Sweden)

    Liu Zan

    2011-08-01

    Full Text Available Abstract Background We have previously constructed an in-depth human glomerulus proteome database from a large amount of sample for understanding renal disease pathogenesis and aiding the biomarker exploration. However, it is usually a challenge for clinical research to get enough tissues for large-scale proteomic characterization. Therefore, in this study, we focused on high-confidence proteomics analysis on small amounts of human glomeruli comparable to those obtained from biopsies using different mass spectrometers and compared these results to the comprehensive database. Results One microgram of human glomerular protein digest was analyzed each on five LC- combined mass spectrometers (LIT-TOF, LTQ-Orbitrap, Q-TOF, LIT and MALDI-TOF/TOF yielding 139, 185, 94, 255 and 108 proteins respectively identified with strict criteria to ensure high confidence (> 99% and low false discovery rate (FDR ( Conclusion This study showed representative human glomerulus proteomic profiles obtained from biopsies through analysis of comparable amounts of samples by different mass spectrometry. Our results implicated that high abundant proteins are more likely to be reproducibly identified in multiple mass spectrometers runs and different mass spectrometers. Furthermore, many podocyte essential proteins such as nephrin, podocin, podocalyxin and synaptopodin were also identified from the small samples in this study. Bioinformatic enrichment analysis results extended our understanding of the major glomerular proteins about their subcellular distributions and functions. The present study indicated that the proteins localized in certain cellular compartments, such as actin cytoskeleton, mitochondrial matrix, cell surface, basolateral plasma membrane, contractile fiber, proteinaceous extracellular matrix and adherens junction, represent high abundant glomerular proteins and these subcellular structures are also highly significantly over-represented in the glomerulus

  13. Statistical evaluation of fatty acid profile and cholesterol content in fish (common carp) lipids obtained by different sample preparation procedures.

    Science.gov (United States)

    Spiric, Aurelija; Trbovic, Dejana; Vranic, Danijela; Djinovic, Jasna; Petronijevic, Radivoj; Matekalo-Sverak, Vesna

    2010-07-01

    the second principal component (PC2) is recorded by C18:3 n-3, and C20:3 n-6, being present in a higher amount in the samples treated by the modified Soxhlet extraction, while C22:5 n-3, C20:3 n-3, C22:1 and C20:4, C16 and C18 negatively influence the score values of the PC2, showing significantly increased level in the samples treated by ASE method. Hotelling's paired T-square test used on the first three principal components for confirmation of differences in individual fatty acid content obtained by ASE and Soxhlet method in carp muscle showed statistically significant difference between these two data sets (T(2)=161.308, p<0.001).

  14. Towards an Understanding of Changing-Look Quasars With a Statistical Sample: An Archival Spectroscopic Search in SDSS

    CERN Document Server

    Ruan, John J; Cales, Sabrina L; Eracleous, Michael; Green, Paul J; Morganson, Eric; Runnoe, Jessie C; Shen, Yue; Wilkinson, Tessa D; Blanton, Michael R; Dwelly, Tom; Georgakakis, Antonis; Greene, Jenny E; LaMassa, Stephanie M; Merloni, Andrea; Schneider, Donald P

    2015-01-01

    The uncertain origin of the recently-discovered 'changing-looking' quasar phenomenon - in which a luminous quasar dims significantly to a quiescent state in repeat spectroscopy over ~10 year timescales - may present unexpected challenges to our understanding of quasar accretion. To better understand this phenomenon, we take a first step to building a statistical sample of changing-look quasars with a systematic but simple archival search for these objects in the Sloan Digital Sky Survey Data Release 12. By leveraging the >10 year baselines for objects with repeat spectroscopy, we uncover two new changing-look quasars. Decomposition of the multi-epoch spectra and analysis of the broad emission lines suggest that the quasar accretion disk emission dims due to rapidly decreasing accretion rates, while disfavoring changes in intrinsic dust extinction. Narrow emission line energetics also support intrinsic dimming of quasar emission as the origin for this phenomenon rather than transient tidal disruption events. A...

  15. Archive of Geosample Data and Information from the Woods Hole Oceanographic Institution (WHOI) Seafloor Samples Laboratory

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Woods Hole Oceanographic Institution (WHOI) Seafloor Samples Laboratory is a partner in the Index to Marine and Lacustrine Geological Samples (IMLGS) database,...

  16. File list: Pol.Dig.50.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.50.AllAg.Gastric_primary_sample hg19 RNA polymerase Digestive tract Gastric... primary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Dig.50.AllAg.Gastric_primary_sample.bed ...

  17. File list: Pol.Dig.20.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.20.AllAg.Gastric_primary_sample hg19 RNA polymerase Digestive tract Gastric... primary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Dig.20.AllAg.Gastric_primary_sample.bed ...

  18. File list: DNS.Dig.50.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Dig.50.AllAg.Gastric_primary_sample hg19 DNase-seq Digestive tract Gastric prim...ary sample SRX201807,SRX201812 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.Dig.50.AllAg.Gastric_primary_sample.bed ...

  19. File list: Pol.Dig.05.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.05.AllAg.Gastric_primary_sample hg19 RNA polymerase Digestive tract Gastric... primary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Dig.05.AllAg.Gastric_primary_sample.bed ...

  20. File list: Oth.Dig.50.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Dig.50.AllAg.Gastric_primary_sample hg19 TFs and others Digestive tract Gastric... primary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Dig.50.AllAg.Gastric_primary_sample.bed ...

  1. File list: Unc.Dig.05.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Dig.05.AllAg.Gastric_primary_sample hg19 Unclassified Digestive tract Gastric p...rimary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.Dig.05.AllAg.Gastric_primary_sample.bed ...

  2. File list: Unc.Dig.50.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Dig.50.AllAg.Gastric_primary_sample hg19 Unclassified Digestive tract Gastric p...rimary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.Dig.50.AllAg.Gastric_primary_sample.bed ...

  3. File list: Oth.Dig.05.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Dig.05.AllAg.Gastric_primary_sample hg19 TFs and others Digestive tract Gastric... primary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Dig.05.AllAg.Gastric_primary_sample.bed ...

  4. File list: DNS.Dig.05.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Dig.05.AllAg.Gastric_primary_sample hg19 DNase-seq Digestive tract Gastric prim...ary sample SRX201807,SRX201812 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.Dig.05.AllAg.Gastric_primary_sample.bed ...

  5. File list: DNS.Dig.20.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Dig.20.AllAg.Gastric_primary_sample hg19 DNase-seq Digestive tract Gastric prim...ary sample SRX201807,SRX201812 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.Dig.20.AllAg.Gastric_primary_sample.bed ...

  6. File list: DNS.Dig.10.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Dig.10.AllAg.Gastric_primary_sample hg19 DNase-seq Digestive tract Gastric prim...ary sample SRX201807,SRX201812 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.Dig.10.AllAg.Gastric_primary_sample.bed ...

  7. File list: Unc.Dig.20.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Dig.20.AllAg.Gastric_primary_sample hg19 Unclassified Digestive tract Gastric p...rimary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.Dig.20.AllAg.Gastric_primary_sample.bed ...

  8. File list: Oth.Dig.20.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Dig.20.AllAg.Gastric_primary_sample hg19 TFs and others Digestive tract Gastric... primary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Dig.20.AllAg.Gastric_primary_sample.bed ...

  9. File list: Oth.Dig.10.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Dig.10.AllAg.Gastric_primary_sample hg19 TFs and others Digestive tract Gastric... primary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Dig.10.AllAg.Gastric_primary_sample.bed ...

  10. File list: Pol.Dig.10.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Dig.10.AllAg.Gastric_primary_sample hg19 RNA polymerase Digestive tract Gastric... primary sample http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Dig.10.AllAg.Gastric_primary_sample.bed ...

  11. File list: NoD.Kid.10.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Kid.10.AllAg.Nephrectomy_sample hg19 No description Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Kid.10.AllAg.Nephrectomy_sample.bed ...

  12. File list: InP.Kid.10.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Kid.10.AllAg.Nephrectomy_sample hg19 Input control Kidney Nephrectomy sample SR...90,SRX1037589,SRX1037588,SRX1037582 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Kid.10.AllAg.Nephrectomy_sample.bed ...

  13. File list: InP.Kid.50.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Kid.50.AllAg.Nephrectomy_sample hg19 Input control Kidney Nephrectomy sample SR...84,SRX1037589,SRX1037590,SRX1037583 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Kid.50.AllAg.Nephrectomy_sample.bed ...

  14. File list: His.Kid.50.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Kid.50.AllAg.Nephrectomy_sample hg19 Histone Kidney Nephrectomy sample SRX95646...X1037580,SRX1037579,SRX956473 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Kid.50.AllAg.Nephrectomy_sample.bed ...

  15. File list: His.Kid.05.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Kid.05.AllAg.Nephrectomy_sample hg19 Histone Kidney Nephrectomy sample SRX95646...1037579,SRX1037580,SRX1037585 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Kid.05.AllAg.Nephrectomy_sample.bed ...

  16. File list: His.Kid.10.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Kid.10.AllAg.Nephrectomy_sample hg19 Histone Kidney Nephrectomy sample SRX95646...1037587,SRX1037586,SRX1037580 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Kid.10.AllAg.Nephrectomy_sample.bed ...

  17. File list: InP.Kid.20.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Kid.20.AllAg.Nephrectomy_sample hg19 Input control Kidney Nephrectomy sample SR...83,SRX1037590,SRX1037588,SRX1037582 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Kid.20.AllAg.Nephrectomy_sample.bed ...

  18. File list: His.Kid.20.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Kid.20.AllAg.Nephrectomy_sample hg19 Histone Kidney Nephrectomy sample SRX95646...1037587,SRX1037580,SRX1037579 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Kid.20.AllAg.Nephrectomy_sample.bed ...

  19. File list: Oth.Kid.10.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Kid.10.AllAg.Nephrectomy_sample hg19 TFs and others Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Kid.10.AllAg.Nephrectomy_sample.bed ...

  20. File list: Oth.Kid.20.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Kid.20.AllAg.Nephrectomy_sample hg19 TFs and others Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Kid.20.AllAg.Nephrectomy_sample.bed ...

  1. File list: NoD.Kid.20.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Kid.20.AllAg.Nephrectomy_sample hg19 No description Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Kid.20.AllAg.Nephrectomy_sample.bed ...

  2. File list: Oth.Kid.05.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Kid.05.AllAg.Nephrectomy_sample hg19 TFs and others Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Kid.05.AllAg.Nephrectomy_sample.bed ...

  3. File list: Oth.Kid.50.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Kid.50.AllAg.Nephrectomy_sample hg19 TFs and others Kidney Nephrectomy sample h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Kid.50.AllAg.Nephrectomy_sample.bed ...

  4. File list: InP.Kid.05.AllAg.Nephrectomy_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Kid.05.AllAg.Nephrectomy_sample hg19 Input control Kidney Nephrectomy sample SR...84,SRX1037590,SRX1037588,SRX1037589 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Kid.05.AllAg.Nephrectomy_sample.bed ...

  5. Detection of a Hobi-like virus in archival samples suggests circulation of this emerging pestivirus species in Europe prior to 2007

    Science.gov (United States)

    The first reported incidence of Hobi-like viruses in Europe dates to a 2010 outbreak of respiratory disease in cattle in Italy. In this study, a Hobi-like virus was detected in archival samples, collected in 2007 in Italy from a cattle herd displaying respiratory disease, during the validation of a...

  6. Identification of pyrimethamine- and chloroquine-resistant Plasmodium falciparum in Africa between 1984 and 1998: genotyping of archive blood samples

    Directory of Open Access Journals (Sweden)

    Saito-Nakano Yumiko

    2011-12-01

    Full Text Available Abstract Background Understanding the geographical distribution of drug resistance of Plasmodium falciparum is important for the effective treatment of malaria. Drug resistance has previously been inferred mainly from records of clinical resistance. However, clinical resistance is not always consistent with the parasite's genetic resistance. Thus, molecular identification of the parasite's drug resistance is required. In Africa, clinical resistance to pyrimethamine (Pyr and chloroquine (CQ was evident before 1980 but few studies investigating the genetic resistance to these drugs were conducted before the late 1990s. In this study, genotyping of genes involved in resistance to Pyr and CQ was performed using archive blood samples from Africa between 1984 and 1998. Methods Parasite DNA was extracted from P. falciparum-infected blood smears collected from travellers returning to Japan from Africa between 1984 and 1998. Genotypes of the dihydrofolate reductase gene (dhfr and CQ-resistance transporter gene (pfcrt were determined by polymerase chain reaction amplification and sequencing. Results Genotyping of dhfr and pfcrt was successful in 59 and 80 samples, respectively. One wild-type and seven mutant dhfr genotypes were identified. Three dhfr genotypes lacking the S108N mutation (NRSI, ICSI, IRSI; amino acids at positions 51, 59, 108, and 164 with mutations underlined were highly prevalent before 1994 but reduced after 1995, accompanied by an increase in genotypes with the S108N mutation. The dhfr IRNI genotype was first identified in Nigeria in 1991 in the present samples, and its frequency gradually increased. However, two double mutants (ICNI and NRNI, the latter of which was exclusively found in West Africa, were more frequent than the IRNI genotype. Only two pfcrt genotypes were found, the wild-type and a Southeast Asian type (CVIET; amino acids at positions 72-76 with mutations underlined. The CVIET genotype was already present as early as

  7. File list: His.Dig.05.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Dig.05.AllAg.Gastric_primary_sample hg19 Histone Digestive tract Gastric primar...105,SRX369094,SRX369095 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Dig.05.AllAg.Gastric_primary_sample.bed ...

  8. File list: His.Dig.20.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Dig.20.AllAg.Gastric_primary_sample hg19 Histone Digestive tract Gastric primar...096,SRX369094,SRX369095 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Dig.20.AllAg.Gastric_primary_sample.bed ...

  9. File list: His.Dig.10.AllAg.Gastric_primary_sample [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Dig.10.AllAg.Gastric_primary_sample hg19 Histone Digestive tract Gastric primar...095,SRX369094,SRX369099 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Dig.10.AllAg.Gastric_primary_sample.bed ...

  10. Collecting, archiving and processing DNA from wildlife samples using FTA® databasing paper

    OpenAIRE

    Burgoyne LA; Smith LM

    2004-01-01

    Abstract Background Methods involving the analysis of nucleic acids have become widespread in the fields of traditional biology and ecology, however the storage and transport of samples collected in the field to the laboratory in such a manner to allow purification of intact nucleic acids can prove problematical. Results FTA® databasing paper is widely used in human forensic analysis for the storage of biological samples and for purification of nucleic acids. The possible uses of FTA® databas...

  11. Sampling

    CERN Document Server

    Thompson, Steven K

    2012-01-01

    Praise for the Second Edition "This book has never had a competitor. It is the only book that takes a broad approach to sampling . . . any good personal statistics library should include a copy of this book." —Technometrics "Well-written . . . an excellent book on an important subject. Highly recommended." —Choice "An ideal reference for scientific researchers and other professionals who use sampling." —Zentralblatt Math Features new developments in the field combined with all aspects of obtaining, interpreting, and using sample data Sampling provides an up-to-date treat

  12. Utility of the Microculture Method in Non-Invasive Samples Obtained from an Experimental Murine Model with Asymptomatic Leishmaniasis

    OpenAIRE

    Allahverdiyev, Adil M; Bagirova, Malahat; Cakir-Koc, Rabia; Elcicek, Serhat; Oztel, Olga Nehir; Canim-Ates, Sezen; Abamor, Emrah Sefik; Yesilkir-Baydar, Serap

    2012-01-01

    The sensitivity of diagnostic methods for visceral leishmaniasis (VL) decreases because of the low number of parasites and antibody amounts in asymptomatic healthy donors who are not suitable for invasive sample acquisition procedures. Therefore, new studies are urgently needed to improve the sensitivity and specificity of the diagnostic approaches in non-invasive samples. In this study, the sensitivity of the microculture method (MCM) was compared with polymerase chain reaction (PCR), enzyme...

  13. Histopathological examination of nerve samples from pure neural leprosy patients: obtaining maximum information to improve diagnostic efficiency.

    Science.gov (United States)

    Antunes, Sérgio Luiz Gomes; Chimelli, Leila; Jardim, Márcia Rodrigues; Vital, Robson Teixeira; Nery, José Augusto da Costa; Corte-Real, Suzana; Hacker, Mariana Andréa Vilas Boas; Sarno, Euzenir Nunes

    2012-03-01

    Nerve biopsy examination is an important auxiliary procedure for diagnosing pure neural leprosy (PNL). When acid-fast bacilli (AFB) are not detected in the nerve sample, the value of other nonspecific histological alterations should be considered along with pertinent clinical, electroneuromyographical and laboratory data (the detection of Mycobacterium leprae DNA with polymerase chain reaction and the detection of serum anti-phenolic glycolipid 1 antibodies) to support a possible or probable PNL diagnosis. Three hundred forty nerve samples [144 from PNL patients and 196 from patients with non-leprosy peripheral neuropathies (NLN)] were examined. Both AFB-negative and AFB-positive PNL samples had more frequent histopathological alterations (epithelioid granulomas, mononuclear infiltrates, fibrosis, perineurial and subperineurial oedema and decreased numbers of myelinated fibres) than the NLN group. Multivariate analysis revealed that independently, mononuclear infiltrate and perineurial fibrosis were more common in the PNL group and were able to correctly classify AFB-negative PNL samples. These results indicate that even in the absence of AFB, these histopathological nerve alterations may justify a PNL diagnosis when observed in conjunction with pertinent clinical, epidemiological and laboratory data.

  14. Histopathological examination of nerve samples from pure neural leprosy patients: obtaining maximum information to improve diagnostic efficiency

    Directory of Open Access Journals (Sweden)

    Sérgio Luiz Gomes Antunes

    2012-03-01

    Full Text Available Nerve biopsy examination is an important auxiliary procedure for diagnosing pure neural leprosy (PNL. When acid-fast bacilli (AFB are not detected in the nerve sample, the value of other nonspecific histological alterations should be considered along with pertinent clinical, electroneuromyographical and laboratory data (the detection of Mycobacterium leprae DNA with polymerase chain reaction and the detection of serum anti-phenolic glycolipid 1 antibodies to support a possible or probable PNL diagnosis. Three hundred forty nerve samples [144 from PNL patients and 196 from patients with non-leprosy peripheral neuropathies (NLN] were examined. Both AFB-negative and AFB-positive PNL samples had more frequent histopathological alterations (epithelioid granulomas, mononuclear infiltrates, fibrosis, perineurial and subperineurial oedema and decreased numbers of myelinated fibres than the NLN group. Multivariate analysis revealed that independently, mononuclear infiltrate and perineurial fibrosis were more common in the PNL group and were able to correctly classify AFB-negative PNL samples. These results indicate that even in the absence of AFB, these histopathological nerve alterations may justify a PNL diagnosis when observed in conjunction with pertinent clinical, epidemiological and laboratory data.

  15. Biological investigations of Indian phaeophyceae: 17. Seasonal variation of antibacterial activity of total sterols obtained from frozen samples of Sargassum johnstonii Setchell et Gardner

    Digital Repository Service at National Institute of Oceanography (India)

    Rao, P.P.S.

    From lipid fraction of frozen samples of Sargassum johnstonii unsaponifiable part was extracted with diethyl ether to isolate total sterols. The extracted sterols were obtained for a period of nine months and tested against test bacteria...

  16. Development of an inventory/archive program for the retention, management, and disposition of tank characterization samples at the 222-S laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Seidel, C.M.

    1998-04-29

    The Hanford Tank Waste Remediation Systems (TWRS) Characterization Program is responsible for coordinating the sampling and analysis of the 177 large underground storage tanks at the Hanford site. The 222-S laboratory has been the primary laboratory for chemical analysis of this highly-radioactive material and has been accumulating these samples for many years. As part of the Fiscal Year 1998 laboratory work scope, the 222-S laboratory has performed a formal physical inventory of all tank characterization samples which are currently being stored. In addition, an updated inventory/archive program has been designed. This program defines sample storage, retention, consolidation, maintenance, and disposition activities which will ensure that the sample integrity is preserved to the greatest practical extent. In addition, the new program provides for continued availability of waste material in a form which will be useful for future bench-scale studies. Finally, when the samples have exceeded their useful lifetime, the program provides for sample disposition from,the laboratory in a controlled, safe and environmentally compliant manner. The 222-S laboratory maintains custody over samples of tank waste material which have been shipped to the laboratory for chemical analysis. The storage of these samples currently requires an entire hotcell, fully dedicated to sample archive storage, and is rapidly encroaching on additional hotcell space. As additional samples are received, they are beginning to limit the 222-S laboratory hotcell utility for other activities such as sample extrusion and subsampling. The 222-S laboratory tracks the number of sample containers and the mass of each sample through an internal database which has recently been verified and updated via a physical inventory.

  17. Development of an inventory/archive program for the retention, management, and disposition of tank characterization samples at the 222-S laboratory

    International Nuclear Information System (INIS)

    The Hanford Tank Waste Remediation Systems (TWRS) Characterization Program is responsible for coordinating the sampling and analysis of the 177 large underground storage tanks at the Hanford site. The 222-S laboratory has been the primary laboratory for chemical analysis of this highly-radioactive material and has been accumulating these samples for many years. As part of the Fiscal Year 1998 laboratory work scope, the 222-S laboratory has performed a formal physical inventory of all tank characterization samples which are currently being stored. In addition, an updated inventory/archive program has been designed. This program defines sample storage, retention, consolidation, maintenance, and disposition activities which will ensure that the sample integrity is preserved to the greatest practical extent. In addition, the new program provides for continued availability of waste material in a form which will be useful for future bench-scale studies. Finally, when the samples have exceeded their useful lifetime, the program provides for sample disposition from,the laboratory in a controlled, safe and environmentally compliant manner. The 222-S laboratory maintains custody over samples of tank waste material which have been shipped to the laboratory for chemical analysis. The storage of these samples currently requires an entire hotcell, fully dedicated to sample archive storage, and is rapidly encroaching on additional hotcell space. As additional samples are received, they are beginning to limit the 222-S laboratory hotcell utility for other activities such as sample extrusion and subsampling. The 222-S laboratory tracks the number of sample containers and the mass of each sample through an internal database which has recently been verified and updated via a physical inventory

  18. Antimicrobial resistance trends among canine Escherichia coli isolates obtained from clinical samples in the northeastern USA, 2004–2011

    OpenAIRE

    Cummings, Kevin J.; Aprea, Victor A.; Altier, Craig

    2015-01-01

    Our objectives were to describe the antimicrobial susceptibility of Escherichia coli isolates from dogs in the northeastern USA and to identify temporal trends in resistance to selected antimicrobial agents. Data were collected retrospectively for all canine E. coli isolates from clinical samples submitted to Cornell University’s Animal Health Diagnostic Center between January 1, 2004 and December 31, 2011. Antimicrobial susceptibility testing was performed on 3519 canine E. coli isolates; fr...

  19. Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus

    DEFF Research Database (Denmark)

    Stenfeldt, Anna Carolina; Heegaard, Peter M. H.; Stockmarr, Anders;

    2012-01-01

    A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN...

  20. An Investigation About the Distribution and Isolation of Pseudomonas from Raw Milk Samples Obtained from Different Areas

    OpenAIRE

    Uraz, Güven; ÇITAK, Sumru

    1998-01-01

    In this research, a total of 48 Pseudomonas had been isolated using PseudomonasCN and CFC Agar, Pseudomonas P(King A) and PseudomonasF(King B) and Crystal Violet Tetrazolium Agar (CVT) from 200 raw milk samples. Of 48 isolated Pseudomonas, (41.66%) 20 have been named as P. fluorescens, (22.51%) 11 as P. aeruginosa,22.91%) 11 as P. putida, (4.16%) 2 as P. aurefa-ciens, (6.25%) 3 as P. pseudomallei, (2.08%) 1 as P. cepacia. When the distribution of Pseudomonas species has been evaluated acc...

  1. Distribution of uranium and thorium in fractionated sediment samples obtained from different locations across Thane Creek area (Mumbai, India)

    International Nuclear Information System (INIS)

    Uranium concentration varies from 9.01 to 12.7, 8.03 to 11.5, 9.1 to 14.0 and 10.1 to 14.2 mg/kg in grab sediment, coarse sand fraction, fine sand fraction, silt and clay fraction respectively. Thorium concentration varies from 20.0 to 29.4, 17.3 to 28.6, 19.6 to 30.4 and 21.1 to 35.9 in grab sediment, coarse sand fraction, fine sand fraction, silt and clay fraction respectively. Uranium and thorium concentrations vary in different size fractionated sediment samples like silt and clay fraction > fine sand fraction > coarse sand fraction. (author)

  2. A high incidence of polymorphic CYP2C19 variants in archival blood samples from Papua New Guinea

    Directory of Open Access Journals (Sweden)

    Hsu Huai-Ling

    2008-09-01

    Full Text Available Abstract There is considerable inter-ethnic variability in the incidence of CYP2C19 genetic poor metabolisers (var/var. About 3 per cent of Caucasians are CYP2C19 var/var. By contrast, an extremely high incidence (70 per cent is observed in the Melanesian island of Vanuatu. The colonisation of the Pacific Islands is believed to have involved migration through Papua New Guinea (PNG, and hence a high incidence may also be expected in this population. The reported incidence in PNG was only 36 per cent, however. PNG is a country of extensive ethnic diversity, and the incidence of the CYP2C19 var/var in other regional populations of PNG is currently not established. In this study, restriction fragment length polymorphism-polymerase chain reaction analysis of archival blood serum samples was used to determine the prevalence of the CYP2C19*2 and *3 variant alleles in three different ethnic and geographically isolated populations of PNG. In the largest population studied (Iruna, the frequency of both variant CYP2C19 alleles was high (0.37 and 0.34, respectively. Specifically, the frequency of the CYP2C19*3 allele was significantly higher than in the PNG (East Sepik population reported previously (0.34 vs 0.16; p 0.0001. In the Iruna population, 48.9 per cent of the samples were homozygous variants for CYP2C19*2 or *3, which although higher was not statistically different from the East Sepik population (36 per cent. The results of this study indicated that other regional populations of PNG also have a relatively high incidence of the CYP2C19 genetic polymorphism compared with Caucasian populations. The high incidence reported in Vanuatu, however, may be due to genetic drift rather than a PNG founder population, as the Vanuatu population is dominated by the CYP2C19*2 allele, with a lower contribution from the *3 allelic variant.

  3. A standardized procedure to obtain mesenchymal stem/stromal cells from minimally manipulated dental pulp and Wharton's jelly samples.

    Science.gov (United States)

    Ducret, M; Fabre, H; Degoult, O; Atzeni, G; McGuckin, C; Forraz, N; Mallein-Gerrin, F; Perrier-Groult, E; Fargues, J C

    2016-01-01

    Transplantation of mesenchymal stem/stromalcells (MSCs) has emerged as an effectivemethod to treat diseased or damagedorgans and tissues, and hundreds of clinicaltrials using MSCs are currently under way todemonstrate the validity of such a therapeuticapproach. However, most MSCs used for clinicaltrials are prepared in research laboratorieswith insufficient manufacturing quality control.In particular, laboratories lack standardizedprocedures for in vitro isolation of MSCs fromtissue samples, resulting in heterogeneouspopulations of cells and variable experimentaland clinical results.MSCs are now referred to as Human CellularTissue-based Products or Advanced TherapyMedicinal Products, and guidelines fromthe American Code of Federal Regulation ofthe Food and Drug Administration (21 CFRPart 1271) and from the European MedicinesAgency (European Directive 1394/2007) definerequirements for appropriate production ofthese cells. These guidelines, commonly called"Good Manufacturing Practices" (GMP),include recommendations about laboratorycell culture procedures to ensure optimal reproducibility,efficacy and safety of the finalmedicinal product. In particular, the Food andDrug Administration divides ex vivo culturedcells into "minimally" and "more than minimally"manipulated samples, in function of theuse or not of procedures "that might alter thebiological features of the cells". Today, minimalmanipulation conditions have not beendefined for the collection and isolation ofMSCs (Torre et al. 2015)(Ducret et al. 2015).Most if not all culture protocols that have beenreported so far are unsatisfactory, becauseof the use of xeno- or allogeneic cell culturemedia, enzymatic treatment and long-termcell amplification that are known to alter thequality of MSCs.The aim of this study was to describe a standardizedprocedure for recovering MSCs withminimal handling from two promising sources,the dental pulp (DP) and the Wharton's jelly(WJ) of the umbilical cord. The quality and

  4. Archived neonatal dried blood spot samples can be used for accurate whole genome and exome-targeted next-generation sequencing

    DEFF Research Database (Denmark)

    Hollegaard, Mads Vilhelm; Grauholm, Jonas; Nielsen, Ronni;

    2013-01-01

    Dried blood spot samples (DBSS) have been collected and stored for decades as part of newborn screening programmes worldwide. Representing almost an entire population under a certain age and collected with virtually no bias, the Newborn Screening Biobanks are of immense value in medical studies...... can be used for accurate whole genome sequencing (WGS) and exome sequencing (WES). This study examined two individuals represented by three different types of samples each: whole-blood (reference samples), 3-year-old DBSS spotted with reference material (refDBSS), and 27- to 29-year-old archived...... neonatal DBSS (neoDBSS) stored at -20°C in the Danish Newborn Screening Biobank. The reference samples were genotyped using an Illumina Omni2.5M array, and all samples were sequenced on a HighSeq2000 Paired-End flow cell. First, we compared the array single nucleotide polymorphism (SNP) genotype data...

  5. The possibilities of atmospheric plasma-spraying application to obtain hydroxyapatite coatings on the stainless steel samples

    Directory of Open Access Journals (Sweden)

    Mihailović Marija D.

    2013-01-01

    Full Text Available For decades, the standard metallic materials for hip implants, besides the 316LVM stainless steel, were titanium- and cobalt/chromium-based alloys. Although bioinert, due to their corrosion resistance, they are not biocompatible. Contemporary surgical implants are not made just of bioinert metal anymore, but with deposited bioactive hydroxyapatite (HAp coating. Hydroxyapatite is chemically identical with the mineral constituent of bones and teeth, what besides its biocompatibility provides bioactivity as well. The HAp limitations are, however, weak tensile strength and low fatigue resistance for long term loadings, if used alone. This is the reason for HAp to be deposited onto the surgical implant, and to enable its bioactivity, what means intergrowth with bones, and therefore the long-lasting and mechanical stable non-cemented prosthesis. This is important predominantly because the need for such prostheses for younger population, and a better life quality. There are several contemporary techniques that have been used for deposition of these coatings onto the metal implant. The possibilities of atmospheric plasma-spraying for obtaining the stable HAp coatings on the 316LVM stainless steel, ordinary used as a standard material for hip implants production are presented in this paper. The coatings of a commercially available hydroxyapatite powder were plasma-sprayed onto the specimens of medical grade 316LVM stainless steel under various operating conditions. The optical microscopy was used for microstructure and porosity characterization, while coating morphology and Ca/P ratio were analyzed using SEM equipped with EDX. Coating microstructure varied from a porous to a glassy structure, depending on operating conditions applied and coating thickness. Coating porosity was determined to be at the lower required limit requested for the bone-coating intergrowth possibility, but nevertheless adhesion measurements showed good results. The Ca/P ratio was

  6. Investigation of false positives associated with loop-mediated isothermal amplification assays for detection of Toxoplasma gondii in archived tissue samples of captive felids.

    Science.gov (United States)

    Suleman, Essa; Mtshali, Moses Sibusiso; Lane, Emily

    2016-09-01

    Toxoplasma gondii is a ubiquitous protozoan parasite that infects humans and many different animals, including felids. Many molecular and serologic tests have been developed for detection of T. gondii in a wide range of hosts. Loop-mediated isothermal amplification (LAMP) is a field-friendly technique that lacks the practical drawbacks of other molecular and serologic tests, and LAMP assays have been successfully developed for detection of T. gondii in fresh tissue samples. In the current study, both a previously published and a de-novo designed LAMP assay were compared to a quantitative real-time (q)PCR assay, for the detection of T. gondii in archived formalin-fixed, paraffin-embedded (FFPE) tissue samples from captive wildlife. The LAMP assays produced conflicting results, generating both false positives and false negatives. Furthermore, the LAMP assays were unable to positively identify samples with low levels of parasites as determined by qPCR and histopathology. Therefore, these LAMP assays may not be the most suitable assays for detection of T. gondii in archived FFPE and frozen tissue samples. PMID:27449130

  7. Limitations in the use of archived vent mussel samples to assess genetic connectivity among seafloor massive sulfide deposits: a case study with implications for environmental management

    Directory of Open Access Journals (Sweden)

    Rachel Elizabeth Boschen

    2015-12-01

    Full Text Available Genetic connectivity studies can inform the design of mitigation strategies used in environmental management. However, the expense of developing species-specific molecular markers and collecting samples at appropriate spatial and temporal scales can be prohibitive. Using archived material and existing molecular markers may provide a cost-effective way to assess population connectivity. Genetic connectivity studies are increasingly in demand in the deep sea in response to mounting anthropogenic pressures, including seafloor massive sulfide (SMS mining. The feasibility of using archived material was assessed using the New Zealand-endemic vent mussel Gigantidas gladius, which inhabits areas licensed for the prospecting phase of SMS mining. Four molecular markers were tested, but only one (mitochondrial COI provided suitable sequences. Of 942 specimens, only 150 individuals were informative, largely due to poor tissue quality of archived samples. Seven populations spanning the distributional range of G. gladius were assessed. The results indicate that G. gladius has high levels of gene flow among sites 10s to 100s km apart and limited genetic structure. Haplotypic diversity was not equally distributed among populations, with lower diversity for the Macauley Volcano population at the northern extent of the species distribution and greater diversity within central populations. Migrant exchange was also greatest between central populations, with one population at Rumble V Seamount appearing important in terms of maintaining genetic diversity within the Kermadec Volcanic Arc region. However, interpretation of the results should be viewed with caution as small sample sizes may have limited the ability to detect genetic structure. Despite these limitations, mitigation strategies that protect areas of seabed from mining activities should consider the genetic vulnerability of the population at the northern edge of the species’ distribution and the

  8. [Use of archival formalin-fixed, paraffin-embedded (FFPE) tissue samples for molecular genetic analysis in diffuse large B-cell lymphoma (DLBCL)].

    Science.gov (United States)

    Jarošová, Marie; Kučerová, Jana; Flodr, Patrik; Mikešová, Michaela; Procházka, Vít; Papajík, Tomáš

    2014-04-01

    The currently valid molecular genetic subclassification of patients with diffuse large B-cell lymphoma (DLBCL) into three prognostic subgroups based on expression profiling has been the objective of numerous genetic studies. In routine clinical practice, however, expression profiling technology remains unavailable for the most of centers. Apart from the technology, in some cases molecular genetic laboratories have problems obtaining high-quality material, i.e. fresh tissues, for RNA isolation to determine gene expression. One possibility is to determine the gene expression from RNA obtained by isolation from formalin-fixed, paraffin-embedded (FFPE) tissue. This pilot study aimed at isolating RNA from FFPE in patients diagnosed with DLBCL and verifying the potential use of such RNA for the expression analysis of 7 selected genes. Although the study showed that it is possible to isolate RNA and determine the expression of the selected genes from archival material, the values of relative expression of some genes in the set were too variable to be used for unambiguous prognostic classification. It was confirmed that retrospective analyses of selected genes may be performed with sufficient material obtained, and that properly archived blocks may be used for molecular biology analyses even after 8 years.

  9. Comparison of geochemical data obtained using four brine sampling methods at the SECARB Phase III Anthropogenic Test CO2 injection site, Citronelle Oil Field, Alabama

    Science.gov (United States)

    Conaway, Christopher; Thordsen, James J.; Manning, Michael A.; Cook, Paul J.; Trautz, Robert C.; Thomas, Burt; Kharaka, Yousif K.

    2016-01-01

    The chemical composition of formation water and associated gases from the lower Cretaceous Paluxy Formation was determined using four different sampling methods at a characterization well in the Citronelle Oil Field, Alabama, as part of the Southeast Regional Carbon Sequestration Partnership (SECARB) Phase III Anthropogenic Test, which is an integrated carbon capture and storage project. In this study, formation water and gas samples were obtained from well D-9-8 #2 at Citronelle using gas lift, electric submersible pump, U-tube, and a downhole vacuum sampler (VS) and subjected to both field and laboratory analyses. Field chemical analyses included electrical conductivity, dissolved sulfide concentration, alkalinity, and pH; laboratory analyses included major, minor and trace elements, dissolved carbon, volatile fatty acids, free and dissolved gas species. The formation water obtained from this well is a Na–Ca–Cl-type brine with a salinity of about 200,000 mg/L total dissolved solids. Differences were evident between sampling methodologies, particularly in pH, Fe and alkalinity. There was little gas in samples, and gas composition results were strongly influenced by sampling methods. The results of the comparison demonstrate the difficulty and importance of preserving volatile analytes in samples, with the VS and U-tube system performing most favorably in this aspect.

  10. Long time storage (archiving of peptide, protein and tryptic digest samples on disposable nano-coated polymer targets for MALDI MS

    Directory of Open Access Journals (Sweden)

    Stefan Bugovsky

    2015-09-01

    Full Text Available Archiving of biological specimens is important due to the importance of reanalysis of already prepared MALDI MS samples or simultaneuous analysis of a sample series. Proteins/peptides and digests were prepared for measurement on a polymer-based metal nano-coated MALDI target and subjected to various storage conditions (−80 °C to RT in a vacuum-sealed pouch and at atmosphere for 6 months. The MS data gathered from these preparations illustrate trends in the aging of different samples and to find optimal storage conditions (−20 or −80 °C in low oxygen environment. The disposable/low cost target proved to be a suitable platform for storage and MALDI MS.

  11. Use of an Ultrasonic/Sonic Driller/Corer to Obtain Sample Powder for CHEMIN, a Combined XRD/XRF Instrument

    Science.gov (United States)

    Chipera, S. J.; Bish, D. L.; Vaniman, D. T.; Sherrit, S.; Bar-Cohen, Y.; Sarrazin, P.; Blake, D. F.

    2003-01-01

    A miniature CHEMIN XRD/XRF (X-Ray Diffraction/X-Ray Fluourescence) instrument is currently being developed for definitive mineralogic analysis of soils and rocks on Mars. One of the technical issues that must be addressed in order to enable XRD analysis on an extraterrestrial body is how best to obtain a representative sample powder for analysis. For XRD powder diffraction analyses, it is beneficial to have a fine-grained sample to reduce preferred orientation effects and to provide a statistically significant number of crystallites to the X-ray beam. Although a 2-dimensional detector as used in the CHEMIN instrument will produce good results with poorly prepared powders, the quality of the data will improve if the sample is fine-grained and randomly oriented. An Ultrasonic/Sonic Driller/Corer (USDC) currently being developed at JPL is an effective mechanism of sampling rock to produce cores and powdered cuttings. It requires low axial load (XRF spectrometer such as CHEMIN, powders obtained from the JPL ultrasonic drill were analyzed and the results were compared to carefully prepared powders obtained using a laboratory bench scale Retsch mill.

  12. Effects of anesthesia with isoflurane on plasma concentrations of adrenocorticotropic hormone in samples obtained from the cavernous sinus and jugular vein of horses.

    Science.gov (United States)

    Carmalt, James L; Duke-Novakovski, Tanya; Schott, Harold C; van der Kolk, Johannes H

    2016-07-01

    OBJECTIVE To determine effects of anesthesia on plasma concentrations and pulsatility of ACTH in samples obtained from the cavernous sinus and jugular vein of horses. ANIMALS 6 clinically normal adult horses. PROCEDURES Catheters were placed in a jugular vein and into the cavernous sinus via a superficial facial vein. The following morning (day 1), cavernous sinus blood samples were collected every 5 minutes for 1 hour (collection of first sample = time 0) and jugular venous blood samples were collected at 0, 30, and 60 minutes. On day 2, horses were sedated with xylazine hydrochloride and anesthesia was induced with propofol mixed with ketamine hydrochloride. Horses were positioned in dorsal recumbency. Anesthesia was maintained with isoflurane in oxygen and a continuous rate infusion of butorphanol tartrate. One hour after anesthesia was induced, the blood sample protocol was repeated. Plasma ACTH concentrations were quantified by use of a commercially available sandwich assay. Generalized estimating equations that controlled for horse and an expressly automated deconvolution algorithm were used to determine effects of anesthesia on plasma ACTH concentrations and pulsatility, respectively. RESULTS Anesthesia significantly reduced the plasma ACTH concentration in blood samples collected from the cavernous sinus. CONCLUSIONS AND CLINICAL RELEVANCE Mean plasma ACTH concentrations in samples collected from the cavernous sinus of anesthetized horses were reduced. Determining the success of partial ablation of the pituitary gland in situ for treatment of pituitary pars intermedia dysfunction may require that effects of anesthesia be included in interpretation of plasma ACTH concentrations in cavernous sinus blood. PMID:27347826

  13. Evaluation of Picture Archiving and Communication System (Pacs As A Process Improvement Sample (Sivas Numune Hospital Application

    Directory of Open Access Journals (Sweden)

    Ali Rıza İnce

    2013-09-01

    Full Text Available It is a realitythatthefirmsproducinggoods or services frequentlybenefited process improvementand similartechniques toachieve their objectives In today'scompetitive environment.Beingprocess-orientedmeans to give weight to the creation of quality but not to the control of quality. In this sense, process improvement is seen as an approach of Total Quality Management targeted. Hospital informationsystems are computer systems that collect andarchive all orthe majority of the hospitaldatafor use inthe assessment.It isa major problem that storage of visualdatawithout lossanddamage and also the being availableof these data as soon as possiblein this system.System that calledPACShas been developedto overcome this problem.PACS is an archiving system that archive the visual dataobtained fromimaging systems in different unitsin a spaceand, allows the transfer of these data to usersat different points when needed.In this study,efficiency of PACS asa process improvementtechniquewas examined by the application ofahospital.It is concluded that the system providea positive contributionto customers and hospitalas an example ofan importantprocess improvement at the evaluationof the results and implementation of the system.

  14. Detection of Mycobacterium avium subspecies paratuberculosis specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland

    Directory of Open Access Journals (Sweden)

    Stephan Roger

    2002-06-01

    Full Text Available Abstract Background Since Mycobacterium avium subspecies paratuberculosis (MAP was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific IS900 insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of Enterobacteriaceae. Results 273 (19.7% of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p >> 0.05 differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. Enterobacteriaceae occur as often in IS900 PCR-positive as in PCR-negative milk samples. Conclusion This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.

  15. Evaluation of the adequacy and diagnostic accuracy of the histology samples obtained with a newly designed 19-gauge EUS histology needle

    Directory of Open Access Journals (Sweden)

    Julio Iglesias-García

    2014-01-01

    Full Text Available Background: Endoscopic ultrasound (EUS-guided fine needle aspiration (FNA is an accurate technique for sampling intraintestinal and extraintestinal lesions. However, cytology possesses certain limitations, which may be overcome if histological specimens are provided to the pathologist. Aim: The aim of the study was to evaluate the accuracy of a newly developed 19G histology needle. Methods: Retrospective analysis of a prospectively collected data base including patients who underwent EUS-guided biopsy with the 19G ProCoreTM histology needle for the evaluation of intraintestinal or extraintestinal lesions. Samples were obtained after one needle pass, recovered into ThinPrep® and processed for histological analysis. Results were compared to the gold standard of surgical histopathology, or global pathological, clinical and radiological assessment, and follow-up in non-operated cases. Results are shown as mean ± SD. Percentage of optimal samples for histological evaluation and the overall diagnostic accuracy were evaluated. Results: 87 patients (mean age 62.9 years, range 25-88 years, 36 woman were included. Lesions mean size was 41.6 ± 21.3 mm. 66 lesions (75.9 % were considered as malignant and 21 (24.1 % as benign. EUS-guided biopsy was feasible in all cases (100 %. Sample quality was adequate for histological assessment in 82 lesions (94.2 %. In the remaining cases the sample was adequate for cell-block evaluation. Sensitivity, specificity, PPV, NPV, and overall accuracy for malignancy were 93.4 %, 100 %, 100 %, 84 %, and 95.4 %, respectively. There were no complications related to the procedure. Conclusion: The EUS-guided biopsy with the 19G histology needle provides with an optimal core sample for histological evaluation allowing a high histopathologic diagnostic accuracy.

  16. Archiving challenges

    OpenAIRE

    Ringersma, J.

    2010-01-01

    Teaching slides on: What is a digital archive? Parties involved in digital archiving Archiving challenges organization of data coherence and persistency access and safety Language archiving software Different users, different needs For: Saami Language Documentation and Revitalization. Winter school, Bodø, Norway

  17. Calcified Pulmonary Nodules Identified in a 350-Year-Old-Joseon Mummy: the First Report on Ancient Pulmonary Tuberculosis from Archaeologically Obtained Pre-modern Korean Samples

    Science.gov (United States)

    2016-01-01

    We found calcified pulmonary nodules in a middle-aged female mummy discovered from 350-yr-old Joseon tomb of Korea. In the CT scan, we found six radiopaque nodules in right lung, through the levels of thoracic vertebrae 1 to 6. We also found presumptive pleural adhesions in right thoracic cavity of CT images. We re-confirmed radiological findings by our post-factum dissection on the same mummy. By the differential diagnosis, we speculate that the radiopaque calcification nodules and associated pleural adhesion could have been caused by tuberculosis. This is the first-ever report on the pulmonary tuberculosis identified in archaeologically obtained, pre-modern Korean samples. PMID:26770051

  18. Dose-Response Analysis of RNA-Seq Profiles in Archival Formalin-fixed paraffin-embedded (FFPE) Samples

    Science.gov (United States)

    Formalin-fixed paraffin-embedded (FFPE) samples provide a vast untapped resource for chemical safety and translational science. To date, genomic profiling of FFPE samples has been limited by poor RNA quality and inconsistent results with limited utility in dose-response assessmen...

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  7. Quantitative Fraction Evaluation of Dermal Collagen and Elastic Fibres in the Skin Samples Obtained in Two Orientations from the Trunk Region

    Directory of Open Access Journals (Sweden)

    Naveen Kumar

    2014-01-01

    Full Text Available Background. Histomorphic evaluation of dermal collagen and elastic fibres was analysed by image analysis technique. The quantification of dermal elements was performed in skin tissues, collected in horizontal and vertical directions from trunk region and discussed under the perspective of consequences of scar related complications. Materials and Method. Total number of 200 skin samples collected from 5 areas of trunk region were processed histologically and subjected to tissue-quant image analysis. Statistical analysis involving mean with SEM and paired t test by SPSS were employed to the percentage values obtained from image analysis. Result. Among the chosen 5 areas of trunk region, abdomen showed the statistically significant difference for both collagen and elastic content between horizontal and vertical orientations (P< 0.05, whereas upper back, presternal, and lateral chest areas showed significant difference (P< 0.05 only for collagen and groin only for elastic content. Conclusion. The differences in the distribution of dermal collagen and elastic fibres in 2 directions of the samples from the same areas might be attributed to final outcome of wound healing process by influencing the appearance and behaviour of scar related complications in the region of trunk.

  8. Endo- and ectoparasites of large whales (Cetartiodactyla: Balaenopteridae, Physeteridae): Overcoming difficulties in obtaining appropriate samples by non- and minimally-invasive methods.

    Science.gov (United States)

    Hermosilla, Carlos; Silva, Liliana M R; Prieto, Rui; Kleinertz, Sonja; Taubert, Anja; Silva, Monica A

    2015-12-01

    Baleen and sperm whales, belonging to the Order Cetartiodactyla, are the largest and heaviest existent mammals in the world, collectively known as large whales. Large whales have been subjected to a variety of conservation means, which could be better monitored and managed if physiological and pathophysiological information, such as pathogen infections, could already be gathered from free-swimming animals instead of carcasses. Parasitic diseases are increasingly recognized for their profound influences on individual, population, and even ecosystem health. Furthermore, a number of parasite species have gained importance as opportunistic neozoan infections in the marine environment. Nonetheless, traditional approaches to study parasitic diseases have been impractical for large whales, since there is no current routine method for the capture and handling of these large animals and there is presently no practical method to obtain blood samples remotely from free-ranging whales. Therefore, we here not only intend to review the endo- and ectoparasite fauna of large whales but also to provide new insights in current available methods for gathering parasitological data by using non- or minimally invasive sampling techniques. We focus on methods, which will allow detailed parasitological studies to gain a broader knowledge on parasitoses affecting wild, free-swimming large whale populations. PMID:26835249

  9. Quantitative fraction evaluation of dermal collagen and elastic fibres in the skin samples obtained in two orientations from the trunk region.

    Science.gov (United States)

    Kumar, Naveen; Kumar, Pramod; Nayak Badagabettu, Satheesha; Prasad, Keerthana; Kudva, Ranjini; Raghuveer, Coimbatore Vasudevarao

    2014-01-01

    Background. Histomorphic evaluation of dermal collagen and elastic fibres was analysed by image analysis technique. The quantification of dermal elements was performed in skin tissues, collected in horizontal and vertical directions from trunk region and discussed under the perspective of consequences of scar related complications. Materials and Method. Total number of 200 skin samples collected from 5 areas of trunk region were processed histologically and subjected to tissue-quant image analysis. Statistical analysis involving mean with SEM and paired t test by SPSS were employed to the percentage values obtained from image analysis. Result. Among the chosen 5 areas of trunk region, abdomen showed the statistically significant difference for both collagen and elastic content between horizontal and vertical orientations (Pcollagen and groin only for elastic content. Conclusion. The differences in the distribution of dermal collagen and elastic fibres in 2 directions of the samples from the same areas might be attributed to final outcome of wound healing process by influencing the appearance and behaviour of scar related complications in the region of trunk. PMID:25328512

  10. Three-way multivariate analysis of metal fractionation results from sediment samples obtained by different sequential extraction procedures and ICP-OES

    International Nuclear Information System (INIS)

    Three typical schemes for metal fractionation were applied to analyse coastal surface sediment samples from Bahia Blanca estuary, where an important industrial emplacement is located. Also, three certified reference materials for total metal concentrations were analysed. The studied metals were cadmium, chromium, copper, lead and zinc because of their hazardous potential and related abundance in the estuary. The concentration of metals was determined by inductively coupled plasma-optical emission spectrometry (ICP-OES). A three-way multivariate analysis was performed in order to obtain a better visualization of the experimental data. The extracted information was used to evaluate the equivalence among the results obtained by the three sequential extraction schemes. The data were analysed by parallel factor analysis (PARAFAC). PARAFAC models with two factors describe appropriately the data sets (explained variance about 54% and core consistency of 100%). The multivariate decomposition showed that the three applied schemes are able to describe equally well the behaviour of the metals in the different sediment fractions

  11. The Herschel Science Archive

    OpenAIRE

    Verdugo, Eva

    2015-01-01

    The Herschel mission required a Science Archive able to serve data to very different users: The own Data Analysis Software (both Pipeline and Interactive Analysis), the consortia of the different instruments and the scientific community. At the same time, the KP consortia were committed to deliver to the Herschel Science Centre,  the processed products corresponding to the data obtained as part of their Science Demonstration Phase and the Herschel Archive should include the capability to...

  12. Detection of a Hobi-like virus in archival samples suggests circulation of this emerging pestivirus species in Europe prior to 2007.

    Science.gov (United States)

    Decaro, Nicola; Mari, Viviana; Lucente, Maria Stella; Sciarretta, Rossana; Elia, Gabriella; Ridpath, Julia F; Buonavoglia, Canio

    2013-12-27

    The first reported incidence of Hobi-like viruses in Europe dates to a 2010 outbreak of respiratory disease in cattle in Italy. In this study, a Hobi-like virus was detected in archival samples, collected in 2007 in Italy from a cattle herd displaying respiratory disease, during the validation of a nested PCR protocol for rapid characterization of bovine pestiviruses. Phylogeny conducted with full-length pestivirus genomes and three informative genomic sequences, placed the strain detected in the samples, Italy-129/07, into the Hobi-like virus branch. Italy-129/07, similar to other Hobi-like viruses isolated in Italy, was more closely related to viruses of South American origin, than Hobi-like viruses of Southeast Asian origin. This suggests a possible introduction of this emerging group of pestiviruses into Italy as a consequence of using contaminated biological products such as fetal bovine serum originating in South America. This report of a Hobi-like virus associated with respiratory disease along with the full-genomic characterization of the virus detected provides new data that contributes to the body of knowledge regarding the epidemiology, pathobiology and genetic diversity of this emerging group of pestiviruses. Importantly, it dates the circulation of Hobi-like viruses in Italy to 2007, at least three years before previous reports.

  13. Intraparenchymal mesenchymal chondrosarcoma of the frontal lobe--a case report and molecular detection of specific gene fusions from archival FFPE sample.

    Science.gov (United States)

    Sajjad, Emir Ahmed; Sikora, Katarzyna; Paciejewski, Tomasz; Garbicz, Filip; Paskal, Wiktor; Szacht, Milena; Grajkowska, Wieslawa; Włodarski, Pawel Krzysztof

    2015-01-01

    Mesenchymal chondrosarcoma is a rare tumor of cartilaginous origin characterized by its bimorphic pattern composed of highly undifferentiated small round cells separated by islands of well-differentiated hyaline cartilage. It exhibits higher malignancy and earlier occurrence in comparison to classic chondrosarcomas. Recently identified HEY1-NCOA2 and IRF2BP2-CDX1 gene fusions confirm their distinct molecular origin and pose a promising diagnostic marker. The majority of cases arise from craniofacial bones. In this study, we present a rare case of mesenchymal chondrosarcoma encompassed within the brain parenchyma of the frontal lobe without any dural or bone attachment. We demonstrate histopathological findings and confirm the HEY1-NCOA2 gene fusion in a formalin-fixed paraffin-embedded archival sample using simple reverse transcription polymerase chain reaction (RT-PCR) method. IRF2BP2-CDX1 gene fusion was absent in the analyzed sample. The clinical follow-up is also presented with a review of treatment modalities for this entity.

  14. ANALYSES OF HTF-48-12-20/24 (FEBRUARY, 2012) AND ARCHIVED HTF-E-05-021 TANK 48H SLURRY SAMPLES

    Energy Technology Data Exchange (ETDEWEB)

    Nash, C.; Peters, T.

    2012-08-02

    Personnel characterized a Savannah River National Laboratory (SRNL) archived sample of Tank 48H slurry (HTF-E-05-021) in addition to the composite of samples HTF-48-12-20 and HTF-48-12-24, which were both retrieved in February 2012. The combined February 2012 sample is referred to as HTF-48-12-20/24 in this report. The results from these analyses are compared with Tank 48H samples analyzed in 2003, 2004, and 2005. This work supports the effort to demonstrate copper-catalyzed peroxide oxidation (CCPO) of organic content in this material. The principal findings with respect to the chemical and physical characteristics of the most recent sample are: (1) The measured potassium tetraphenylborate (KTPB) solid concentration is 1.76 wt %; (2) Titanium was in line with 2004 and 2005 slurry measurements at 897 mg/L, it represents 0.1535 {+-} 0.0012 wt % monosodium titanate (MST); (3) The measured insoluble solids content was 1.467 wt %; (4) The free hydroxide concentration in the Tank 48H filtrate sample (1.02 {+-} 0.02 M) is close to the Tank 48H limit (1.0 M); (5) Carbonate reported by total inorganic carbon (TIC, 1.39 {+-} 0.03 M) is more than double the concentrations measured in past (2003-2005) samples; (6) The soluble potassium content (measured at 286 {+-} 23 mg/L) in the filtrate is in line with all past measurements; and (7) The measured {sup 137}Cs concentration is 7.81E + 08 {+-} 3.9E + 07 dpm/mL of slurry (1.33 {+-} 5% Ci/gallon or 3.18E + 05 {+-} 5% curies of {sup 137}Cs in the tank) in the slurry which is in agreement with the 2005 report of 3.14E + 05 {+-} 1.5% curies of {sup 137}Cs in the tank. The filtrate {sup 137}Cs concentration is 2.57E + 07 {+-} 2.6E + 05 dpm/mL. This result is consistent with previous results. Significant analytical data are summarized in Table 1.

  15. Determining the utility of veterinary tissue archives for retrospective DNA analysis

    Science.gov (United States)

    Abed, Firas M.

    2016-01-01

    Histopathology tissue archives can be an important source of specimens for retrospective studies, as these include samples covering a large number of diseases. In veterinary medicine, archives also contain samples from a large variety of species and may represent naturally-occurring models of human disease. The formalin-fixed, paraffin-embedded (FFPE) tissues comprising these archives are rich resources for retrospective molecular biology studies and pilot studies for biomarkers, as evidenced by a number of recent publications highlighting FFPE tissues as a resource for analysis of specific diseases. However, DNA extracted from FFPE specimens are modified and fragmented, making utilization challenging. The current study examines the utility of FFPE tissue samples from a veterinary diagnostic laboratory archive in five year intervals from 1977 to 2013, with 2015 as a control year, to determine how standard processing and storage conditions has affected their utility for future studies. There was a significant difference in our ability to obtain large amplicons from samples from 2015 than from the remaining years, as well as an inverse correlation between the age of the samples and product size obtainable. However, usable DNA samples were obtained in at least some of the samples from all years tested, despite variable storage, fixation, and processing conditions. This study will help make veterinary diagnostic laboratory archives more useful in future studies of human and veterinary disease. PMID:27168995

  16. Determining the utility of veterinary tissue archives for retrospective DNA analysis.

    Science.gov (United States)

    Abed, Firas M; Dark, Michael J

    2016-01-01

    Histopathology tissue archives can be an important source of specimens for retrospective studies, as these include samples covering a large number of diseases. In veterinary medicine, archives also contain samples from a large variety of species and may represent naturally-occurring models of human disease. The formalin-fixed, paraffin-embedded (FFPE) tissues comprising these archives are rich resources for retrospective molecular biology studies and pilot studies for biomarkers, as evidenced by a number of recent publications highlighting FFPE tissues as a resource for analysis of specific diseases. However, DNA extracted from FFPE specimens are modified and fragmented, making utilization challenging. The current study examines the utility of FFPE tissue samples from a veterinary diagnostic laboratory archive in five year intervals from 1977 to 2013, with 2015 as a control year, to determine how standard processing and storage conditions has affected their utility for future studies. There was a significant difference in our ability to obtain large amplicons from samples from 2015 than from the remaining years, as well as an inverse correlation between the age of the samples and product size obtainable. However, usable DNA samples were obtained in at least some of the samples from all years tested, despite variable storage, fixation, and processing conditions. This study will help make veterinary diagnostic laboratory archives more useful in future studies of human and veterinary disease. PMID:27168995

  17. Gaia archive

    CERN Document Server

    Hypki, Arkadiusz

    2016-01-01

    The Gaia archive is being designed and implemented by the DPAC Consortium. The purpose of the archive is to maximize the scientific exploitation of the Gaia data by the astronomical community. Thus, it is crucial to gather and discuss with the community the features of the Gaia archive as much as possible. It is especially important from the point of view of the GENIUS project to gather the feedback and potential use cases for the archive. This paper presents very briefly the general ideas behind the Gaia archive and presents which tools are already provided to the community.

  18. Archival Descriptions from the National Archives Catalog

    Data.gov (United States)

    National Archives and Records Administration — Archival Descriptions from the National Archives Catalog data set provides archival descriptions of the permanent holdings of the federal government in the custody...

  19. Age structure of yellow-necked mouse (Apodemus flavicollis Melchior, 1834 in two samples obtained from live traps and owl pellets

    Directory of Open Access Journals (Sweden)

    Vukićević-Radić Olivera

    2005-01-01

    Full Text Available The age structure of yellow-necked mouse (Apodemus flavicollis Melchior 1834 has been analyzed in individuals obtained by two methods: Trapping with Sherman live traps and obtainment of skulls from long-eared owl (Asio otus Linnaeus, 1758 pellets (predator diet analysis. One hundred and forty-four mice were analyzed for the degree of wear of the surface of molar crowns, and an additional 74 measurements were performed on captive-born Apodemus flavicollis individuals. We used a refined model of comparison that included seven classes of mouse age, rather than four classes, as suggested by other authors.

  20. Inorganic profile of some Brazilian medicinal plants obtained from ethanolic extract and ''in natura'' samples

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, M.O.M.; de Sousa, P.T.; Salvador, V.L.R.; Sato, I.M.

    2004-10-03

    The Anadenathera macrocarpa, Schinus molle, Hymenaea courbaril, Cariniana legalis, Solidago microglossa and Stryphnodendron barbatiman, were collected ''in natura'' samples (leaves, flowers, barks and seeds) from different commercial suppliers. The pharmaco-active compounds in ethanolic extracts had been made by the Mato Grosso Federal University (UFMT). The energy-dispersive x-ray fluorescence (ED-XRF) spectrometry was used for the elemental analysis in different parts of the plants and respective ethanolic extracts. The Ca, Cl, Cu, Fe, K, Mg, Mn, Na, Ni, P, Rb, S, Sr and Zn concentrations were determined by the fundamental parameters method. Some specimens showed a similar inorganic profile for ''in natura'' and ethanolic extract samples and some ones showed a distinct inorganic profile. For example, the Anadenathera macrocarpa showed a similar concentration in Mg, P, Cu, Zn and Rb elements in ''in natura'' and ethanolic extract samples; however very different concentration in Na, S, Cl, K , Ca, Mn, Fe and Sr was observed in distinctive samples. The Solidago microglossa showed the K, Ca, Cl, S, Mg, P and Fe elements as major constituents in both samples, suggesting that the extraction process did not affect in a considerable way the ''in natura'' inorganic composition. The elemental composition of the different parts of the plants (leaves, flowers, barks and seeds) has been also determined. For example, the Schinus molle specimen showed P, K, Cl and Ca elements as major constituents in the seeds, Mg, K and Sr in the barks and Mg, S, Cl and Mn in the leaves, demonstrating a differentiated elementary distribution. These inorganic profiles will contribute to evaluate the quality control of the Brazilian herbaceous trade and also will assist to identify which parts of the medicinal plants has greater therapeutic effect.

  1. Archiving Websites

    DEFF Research Database (Denmark)

    Brügger, Niels

    This book treats the micro archiving of websites, i.e. archiving by researchers, students or others without special technical knowledge who, using a standard computer, wish to save a website for further study. The phenomenon is discussed from the standpoint that Internet research must be able...... to stabilise and save the object of its analysis. However, the Internet is endowed with certain fundamental media-specific dynamics that make stabilisation difficult. Based on an account and discussion of these dynamics (linked as they are to sender, text and recipient) the following double conclusion...... is reached. Firstly, unlike other well-known media, the Internet does not simply exist in a form suited to being archived, but rather is first formed as an object of study in the archiving, and it is formed differently depending on who does the archiving, when, and for what purpose. Secondly, this means...

  2. Detection of foot-and-mouth disease virus RNA in pharyngeal epithelium biopsy samples obtained from infected cattle: Investigation of possible sites of virus replication and persistence

    DEFF Research Database (Denmark)

    Stenfeldt, Anna Carolina; Belsham, Graham

    2012-01-01

    Foot-and-mouth disease (FMD) is a highly contagious viral infection of significant financial importance to the export and trade of agricultural products. The occurrence of persistently infected ‘‘carriers’’ of FMD-virus (FMDV) in ruminant species adds further complications to disease control....... There have been significant discrepancies in reports regarding the pathogenesis of FMDV infection in cattle with specific emphasis on the anatomical sites involved in early and persistent virus replication. In this study, collection of small biopsy samples from the dorsal soft palate (DSP) of live animals...... was used to investigate the level of FMDV RNA present at this site at sequential time points during the infection. Results were compared to measurements of virus excretion in samples of oropharyngeal fluid collected at corresponding time points. Possible sites of virus persistence were investigated through...

  3. Quantitative Fraction Evaluation of Dermal Collagen and Elastic Fibres in the Skin Samples Obtained in Two Orientations from the Trunk Region

    OpenAIRE

    Naveen Kumar; Pramod Kumar; Satheesha Nayak Badagabettu; Keerthana Prasad; Ranjini Kudva; Coimbatore Vasudevarao Raghuveer

    2014-01-01

    Background. Histomorphic evaluation of dermal collagen and elastic fibres was analysed by image analysis technique. The quantification of dermal elements was performed in skin tissues, collected in horizontal and vertical directions from trunk region and discussed under the perspective of consequences of scar related complications. Materials and Method. Total number of 200 skin samples collected from 5 areas of trunk region were processed histologically and subjected to tissue-quant image...

  4. Phenotypic and Molecular Characterisation of Extended-Spectrum Beta-Lactamase Producing Escherichia coli Obtained from Animal Fecal Samples in Ado Ekiti, Nigeria

    OpenAIRE

    Olugbenga Adekunle Olowe; Olufunmilayo Adewumi; Gbolabo Odewale; Olusola Ojurongbe; Olusolabomi Jose Adefioye

    2015-01-01

    Production of extended-spectrum β-lactamases (ESBLs) producing E. coli in animals and different methods of identifications from Ado Ekiti, Ekiti State, Nigeria, were investigated. Three hundred and fifty fecal samples, collected from apparently healthy cattle and pigs, were cultured and identified following standard procedures. ESBL phenotypic detection was carried out using combination disc test, double disc synergism test, and ESBL brilliance agar screening. Molecular detection of TEM, SHV,...

  5. An evaluation of multiplex bead-based analysis of cytokines and soluble proteins in archived lithium heparin plasma, EDTA plasma and serum samples

    DEFF Research Database (Denmark)

    Brøndum, Line; Sørensen, Brita Singers; Eriksen, Jesper Grau;

    2016-01-01

    OBJECTIVE: To assess the usability of archived plasma and serum by multiplex (Luminex) analysis of circulating proteins (analytes) by evaluating the day to day variation, the effect of several freeze-thaw cycles, and the influence of the media and choice of anticoagulant. METHODS: Nineteen analyt...

  6. Thermal history and age of geological regions obtained by applying the fission track method in apatite samples; Historia termica e idades de regioes geologicas obtidas atraves de tracos de fissao em apatita

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Cleber J.; Tello Saenz, Carlos A.; Balan, Ana M. Osorio A.; Dias, Airton N.C.; Novaes, Felipe P.; Oliveira, Roger D.; Barra, Beatriz C., E-mail: pccj13@yahoo.com.b, E-mail: amoa@fct.unesp.b [UNESP, Presidente Prudente, SP (Brazil). Dept. de Fisica, Quimica e Biologia

    2007-07-01

    The objective of this work is dating apatite samples using the Fission Track Method (FTM). Samples were collected from different altitudes in Serra da Mantiqueira (Brazil): High Mantiqueira (above 1,000 m) and Low Mantiqueira (below 1,000 m). Data obtained for the ages and thermal history of the samples via FTM have been interpreted and, according to the geological point of view, can be associated with the uplifting of Serra da Mantiqueira after the opening of the Atlantic Ocean

  7. Phenotypic and Molecular Characterisation of Extended-Spectrum Beta-Lactamase Producing Escherichia coli Obtained from Animal Fecal Samples in Ado Ekiti, Nigeria

    Directory of Open Access Journals (Sweden)

    Olugbenga Adekunle Olowe

    2015-01-01

    Full Text Available Production of extended-spectrum β-lactamases (ESBLs producing E. coli in animals and different methods of identifications from Ado Ekiti, Ekiti State, Nigeria, were investigated. Three hundred and fifty fecal samples, collected from apparently healthy cattle and pigs, were cultured and identified following standard procedures. ESBL phenotypic detection was carried out using combination disc test, double disc synergism test, and ESBL brilliance agar screening. Molecular detection of TEM, SHV, and CTX-M genes was carried out using standard molecular method. One hundred and fourteen E. coli isolates were recovered from the 350 samples processed, out of which 72 (63.2% isolates were positive for ESBLs with multiple resistance to the antibiotics used. Eighty-one (71% isolates were positive for ESBL by combination disc test, 90 (78.9% were positive for double disc synergism test, and 93 (81.6% were positive for ESBL brilliance agar. TEM and CTX-M genes were detected in 48 (42.1% and 51 (44.7% isolates, respectively. SHV gene was not detected in any of the isolates while TEM and CTX-M were detected in 33 (28.9% isolates. This study showed high resistance of E. coli to antibiotics, particularly to the third generation cephalosporins. Regular monitoring and regulated use of antibiotics in livestock should be encouraged.

  8. Thermal and X-ray fluorescences analysis and neutron activation analysis of a soft rock sample obtained from a location in Himalayan ranges

    International Nuclear Information System (INIS)

    Many parts of the Himalayan Ranges are marked by the presence of soft soil. These rocks are known for ages to be full of minerals and herbs. Therefore, an attempt was made to explore the possibility of existence of some interesting elements and to study the thermal and radiation properties of the rock. X-Ray fluorescence analysis and neutron activation analysis indicated presence of some heavy elements such as lead, Zirconium etc. The rock was thermally stable up to a temperature of 1073 K. However a mass loss of 1.1% was recorded due to loss of trapped gases. Presence of lead in the sample and mass loss due to trapped gases indicates possibility existence of uranium in the rock. Specific heat of the rock mineral was found to increase with temperature

  9. Source apportionment by PMF on elemental concentrations obtained by PIXE analysis of PM10 samples collected at the vicinity of lignite power plants and mines in Megalopolis, Greece

    Energy Technology Data Exchange (ETDEWEB)

    Manousakas, M. [Department of Chemistry, University of Patras, 26500 Patras, Achaia (Greece); E.R.L., Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, N.C.S.R. Demokritos, 15310 Ag. Paraskevi, Attiki (Greece); Diapouli, E. [E.R.L., Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, N.C.S.R. Demokritos, 15310 Ag. Paraskevi, Attiki (Greece); Papaefthymiou, H. [Department of Chemistry, University of Patras, 26500 Patras, Achaia (Greece); Migliori, A.; Karydas, A.G.; Padilla-Alvarez, R.; Bogovac, M.; Kaiser, R.B. [Nuclear Science and Instrumentation Laboratory (NSIL), International Atomic Energy Agency, 2400 Seibersdorf (Austria); Jaksic, M.; Bogdanovic-Radovic, I. [Ruder Boskovic Institute, Bijenicka 54, P.O. Box 180, 10002 Zagreb (Croatia); Eleftheriadis, K. [E.R.L., Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, N.C.S.R. Demokritos, 15310 Ag. Paraskevi, Attiki (Greece)

    2015-04-15

    Particulate matter (PM) is an important constituent of atmospheric pollution especially in areas under the influence of industrial emissions. Megalopolis is a small city of 10,000 inhabitants located in central Peloponnese in close proximity to three coal opencast mines and two lignite fired power plants. 50 PM{sub 10} samples were collected in Megalopolis during the years 2009–11 for elemental and multivariate analysis. For the elemental analysis PIXE was used as one of the most effective techniques in APM analytical characterization. Altogether, the concentrations of 22 elements (Z = 11–33), whereas Black Carbon was also determined for each sample using a reflectometer. Factorization software was used (EPA PMF 3.0) for source apportionment analysis. The analysis revealed that major emission sources were soil dust 33% (7.94 ± 0.27 μg/m{sup 3}), biomass burning 19% (4.43 ± 0.27 μg/m{sup 3}), road dust 15% (3.63 ± 0.37 μg/m{sup 3}), power plant emissions 13% (3.01 ± 0.44 μg/m{sup 3}), traffic 12% (2.82 ± 0.37 μg/m{sup 3}), and sea spray 8% (1.99 ± 0.41 μg/m{sup 3}). Wind trajectories have suggested that metals associated with emission from the power plants came mainly from west and were connected with the locations of the lignite mines located in this area. Soil resuspension, road dust and power plant emissions increased during the warm season of the year, while traffic/secondary, sea spray and biomass burning become dominant during the cold season.

  10. Characterization of extended-spectrum β-lactamase (ESBL)-producing Klebsiella, Enterobacter, and Citrobacter obtained in environmental samples of a Tunisian hospital.

    Science.gov (United States)

    Dziri, Raoudha; Klibi, Naouel; Alonso, Carla Andrea; Said, Leila Ben; Bellaaj, Ridha; Slama, Karim Ben; Boudabous, Abdellatif; Torres, Carmen

    2016-10-01

    The assessment of the hospital environment as a reservoir of ESBL-producing Enterobacteriaceae in Tunisian hospitals is scarcely analyzed, except for Escherichia coli. The aim of this study was to evaluate the presence of ESBL-producing non-E. coli Enterobacteriaceae (ESBL-EbNoEc) in 300 samples of abiotic surfaces and the hands of patients and staff of a Tunisian Hospital, and to characterize the ESBL genes of the recovered isolates. ESBL-EbNoEc were recovered in 28 of 300 (9.3%) analyzed samples and were identified as Klebsiella pneumoniae (n= 11), Enterobacter cloacae (n=11), Citrobacter freundii (n=4) and Klebsiella oxytoca (n=2). The bla genes identified by PCR and sequencing among the strains were as follows: 11 K.pneumoniae strains [blaCTX-M-15+ blaTEM-1+ blaSHV-11 (n=6); blaCTX-M-15+ blaTEM-1+ blaSHV-28 (n=3); blaCTX-M-15+ blaTEM-1+ blaSHV-1 (n=2)], 11 E. cloacae strains [blaCTX-M-15 (n=6); blaCTX-M-15+ blaTEM-1b (n=2); blaCTX-M-15+ blaTEM-1b+ blaOXA-1 (n=1);blaCTX-M-15+ blaOXA-1 (n=1);blaSHV-12 (n=1)], 4 C. freundii strains [blaCTX-M-15] and 2 K. oxytoca strains [blaCTX-M-15 (n=1); blaSHV-12 (n=1)]. The ISEcp1 and orf477 sequences were identified upstream and downstream of the blaCTX-M-15 gene, respectively, in 3 K. pneumoniae and 3 E. cloacae isolates. The PFGE analysis demonstrated three unrelated pulsotypes in K. pneumoniae strains and five pulsotypes in E. cloacae. The uncontrolled dissemination of ESBL-producing bacteria, even in the hospital environment, has become a real problem and new strategies and hygienic rules are needed to stop this bacterial dissemination. PMID:27492133

  11. Genetic Sample Inventory

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This database archives genetic tissue samples from marine mammals collected primarily from the U.S. east coast. The collection includes samples from field programs,...

  12. Genetic Sample Inventory - NRDA

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This database archives genetic tissue samples from marine mammals collected in the North-Central Gulf of Mexico from 2010-2015. The collection includes samples from...

  13. Visualizing archives: Spanish archives map

    OpenAIRE

    Colmenero-Ruiz, María-Jesús

    2016-01-01

    The 2014/2015 academic year was the first to be taught the Master in Archives, Records Management and Digital Continuity at the University Carlos III Madrid, based on a blended learning model of attendance. This article describes the implementation of a visualization project based on geolocation of Spanish archives and its justification. The project arose from an optional practice proposed to the students of the subject “Re-use of data and documents” of this master course. This subject is opt...

  14. NASA Data Archive Evaluation

    Science.gov (United States)

    Holley, Daniel C.; Haight, Kyle G.; Lindstrom, Ted

    1997-01-01

    The purpose of this study was to expose a range of naive individuals to the NASA Data Archive and to obtain feedback from them, with the goal of learning how useful people with varied backgrounds would find the Archive for research and other purposes. We processed 36 subjects in four experimental categories, designated in this report as C+R+, C+R-, C-R+ and C-R-, for computer experienced researchers, computer experienced non-researchers, non-computer experienced researchers, and non-computer experienced non-researchers, respectively. This report includes an assessment of general patterns of subject responses to the various aspects of the NASA Data Archive. Some of the aspects examined were interface-oriented, addressing such issues as whether the subject was able to locate information, figure out how to perform desired information retrieval tasks, etc. Other aspects were content-related. In doing these assessments, answers given to different questions were sometimes combined. This practice reflects the tendency of the subjects to provide answers expressing their experiences across question boundaries. Patterns of response are cross-examined by subject category in order to bring out deeper understandings of why subjects reacted the way they did to the archive. After the general assessment, there will be a more extensive summary of the replies received from the test subjects.

  15. The Brightest Galaxies at Cosmic Dawn: Securing the Largest Samples of z=9-11 galaxies for JWST by leveraging the HST archive with Spitzer/IRAC.

    Science.gov (United States)

    Bouwens, Rychard; Trenti, Michele; Calvi, Valentina; Bernard, Stephanie; Labbe, Ivo; Oesch, Pascal; Coe, Dan; Holwerda, Benne; Bradley, Larry; Mason, Charlotte; Schmidt, Kasper; Illingworth, Garth

    2015-10-01

    Hubble's WFC3 has been a game changer for studying early galaxy formation in the first 700 Myr after the Big Bang. Reliable samples of sources up to z~10, which can be discovered only from space, are now constraining the evolution of the galaxy luminosity function into the epoch of reionization. Despite these efforts, the size of the highest redshift galaxy samples (z >9 and especially z > 10) is still very small, particularly at high luminosities (L > L*). To deliver transformational results, much larger numbers of bright z > 9 galaxies are needed both to map out the bright end of the luminosity/mass function and for spectroscopic follow-up (with JWST and otherwise). One especially efficient way of expanding current samples is (1) to leverage the huge amounts of pure-parallel data available with HST to identify large numbers of candidate z ~ 9 - 11 galaxies and (2) to follow up each candidate with shallow Spitzer/IRAC observations to distinguish the bona- fide z ~ 9 - 11 galaxies from z ~ 2 old, dusty galaxies. For this program we are requesting shallow Spitzer/IRAC follow-up of 20 candidate z ~ 9 - 11 galaxies we have identified from 130 WFC3/IR pointings obtained from more than 4 separate HST programs with no existing IRAC coverage. Based on our previous CANDELS/GOODS searches, we expect to confirm 5 to 10 sources as L > L* galaxies at z >= 9. Our results will be used to constrain the bright end of the LF at z >= 9, to provide targets for Keck spectroscopy to constrain the ionization state of the z > 8 universe, and to furnish JWST with bright targets for spectroscopic follow-up studies.

  16. Human archives

    DEFF Research Database (Denmark)

    Poulsen, Bo

    2016-01-01

    explore some of the prospects for including historical archival material further in future research in marine environmental history. Accessing these types of data can be a rewarding but labour intensive adventure. Nonetheless, the ongoing process of digitization is a welcome proliferation...

  17. Chemical and isotopic characteristics of gas hydrate- and pore-water samples obtained from gas hydrate-bearing sediment cores retrieved from a mud volcano in the Kukuy Canyon, Lake Baikal

    Energy Technology Data Exchange (ETDEWEB)

    Minami, H.; Hachikubo, A.; Krylov, A.; Sakagami, H.; Ohashi, M.; Bai, J.; Kataoka, S.; Yamashita, S.; Takahashi, N.; Shoji, H. [Kitami Inst. of Technology, Kitami (Japan); Khlystov, O.; Zemskaya, T.; Grachev, M. [Russian Academy of Sciences, Irkutsk (Russian Federation). Limnological Inst.

    2008-07-01

    This paper provided details of a method developed to obtain gas hydrate water samples from a mud volcano in Lake Baikal, Russia. Chemical and isotopic analyses were conducted to examine the hydrate and pore water samples as well as to evaluate the original water involved in shallow gas hydrate accumulations in the region. Lake sediment core samples were retrieved from the bottom of the lake with gravity corers. A squeezer was used to take pore water samples from the sediments. Hydrate samples were taken from a gas hydrate placed on a polyethylene funnel. Dissolved hydrate water was filtered through a membrane into bottles. Both samples were kept under chilled or liquid nitrogen temperatures. Ion chromatography was used to determine concentrations of anions and hydrogen carbonate ions. Sodium and magnesium concentrations were determined using an inductively coupled plasma atomic emission spectrometer. An absorption spectrometer was used to determine potassium and calcium concentrations, and a mass spectrometer was used to analyze stable isotopes of oxygen and hydrogen. Results of the study suggested that the gas dissolved in pore water and adsorbed on the surfaces of sediment particles was not the original gas from the hydrates retrieved at the mud volcano. Original gas hydrate-forming fluids were chemically different from the pore- and lake-water samples. The oxygen isotopic composition of the gas hydrate water samples correlated well with hydrogen values. It was concluded that ascending fluid and water delivered the gas into the gas stability zone, and is the main gas hydrate-forming fluid in the area of study. 12 refs., 1 fig.

  18. Performing Archives/Archives of Performance

    DEFF Research Database (Denmark)

    Performing Archives/Archives of Performance contributes to the ongoing critical discussions of performance and its disappearance, of the ephemeral and its reproduction, of archives and mediatized recordings of liveness. The many contributions by excellent scholars and artists from a broad range o...... as discussions of specific art works, performances, and archives......Performing Archives/Archives of Performance contributes to the ongoing critical discussions of performance and its disappearance, of the ephemeral and its reproduction, of archives and mediatized recordings of liveness. The many contributions by excellent scholars and artists from a broad range...... of interdisciplinary fields as well as from various locations in research geographies demonstrate that despite the extensive discourse on the relationship between performance and the archive, inquiry into the productive tensions between ephemerality and permanence is by no means outdated or exhausted. New ways...

  19. Online Archives

    Directory of Open Access Journals (Sweden)

    Julian D. Richards

    2004-01-01

    Full Text Available Online archives are of increasing importance in Archaeological Informatics, but like any new genre they prompt a number of questions. What is their relationship to publication? What should go in them? How should they be delivered and indexed? Can they be preserved? Whilst their delivery requires technology, we must also consider how that technology should best be employed in the service of our discipline. This article attempts to address some of these questions but it need not be read from start to finish. The links from this summary provide one fairly linear route through the text but each section is self-contained and can also be accessed directly from the Contents page. The problems posed by effective publication of archaeological fieldwork have exercised the profession for many decades. The issues raised go to the core of discussion of whether preservation by record is a valid concept, and indeed whether archaeological data exist. There are different schools of thought about the relationship between publication and archiving, but hopefully we can accept that data are recorded observations but still agree that they have a re-use value for re-examination and re-interpretation. Since the 1960s archaeology has experienced a publication crisis point. The scenario is familiar. Excavation is destruction; it is an unrepeatable experiment and the archaeologist has a professional obligation to make a full and accessible record of his/her observations. Yet full publication is increasingly expensive and difficult, and excavation monographs are read by few people, and bought by even fewer. In England the development of post-PPG16 fieldwork has exacerbated the problem by creating a mountain of unpublished grey literature and making the work of synthesis even harder; a similar situation exists in other countries. Meanwhile museum archives are also reaching breaking point; most are running out of storage space, few can provide facilities for access, and

  20. National Archives Catalog and API

    Data.gov (United States)

    National Archives and Records Administration — The National Archives Catalog is the online catalog of NARA's nationwide holdings in the Washington, DC area, Regional Archives, and Presidential Libraries.

  1. Constructing an Archive

    DEFF Research Database (Denmark)

    Carbone, Claudia; Wieczorek, Izabela; Francis, Alice;

    2016-01-01

    "Constructing an Archive" contains a collection of student works conducted during the Fall semester of 2015 at the Aarhus School of Architecture, at the master studio Constructing an Archive......."Constructing an Archive" contains a collection of student works conducted during the Fall semester of 2015 at the Aarhus School of Architecture, at the master studio Constructing an Archive....

  2. Archives and the computer

    CERN Document Server

    Cook, Michael Garnet

    1986-01-01

    Archives and the Computer deals with the use of the computer and its systems and programs in archiving data and other related materials. The book covers topics such as the scope of automated systems in archives; systems for records management, archival description, and retrieval; and machine-readable archives. The selection also features examples of archives from different institutions such as the University of Liverpool, Berkshire County Record Office, and the National Maritime Museum.The text is recommended for archivists who would like to know more about the use of computers in archiving of

  3. An Archive of Spectra from the Mayall Fourier Transform Spectrometer at Kitt Peak

    CERN Document Server

    Pilachowski, C A; Young, M D; Dennis, H B; Gopu, A; Henschel, R; Hayashi, S

    2016-01-01

    We describe the SpArc science gateway for spectral data obtained during the period from 1975 through 1995 at the Kitt Peak National Observatory using the Fourier Transform Spectrometer (FTS) in operation at the Mayall 4-m telescope. SpArc is hosted by Indiana University Bloomington and is available for public access. The archive includes nearly 10,000 individual spectra of more than 800 different astronomical sources including stars, nebulae, galaxies, and Solar System objects. We briefly describe the FTS instrument itself, and summarize the conversion of the original interferograms into spectral data and the process for recovering the data into FITS files. The architecture of the archive is discussed, and the process for retrieving data from the archive is introduced. Sample use cases showing typical FTS spectra are presented.

  4. Archive of Geosample Data and Information from the University of Rhode Island (URI) Graduate School of Oceanography (GSO), Marine Geological Samples Laboratory (MGSL)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Marine Geological Samples Laboratory (MGSL) of the Graduate School of Oceanography (GSO), University of Rhode Island is a partner in the Index to Marine and...

  5. Evaluation of the Biofire FilmArray BioThreat-E Test (v2.5) for Rapid Identification of Ebola Virus Disease in Heat-Treated Blood Samples Obtained in Sierra Leone and the United Kingdom.

    Science.gov (United States)

    Weller, Simon A; Bailey, Daniel; Matthews, Steven; Lumley, Sarah; Sweed, Angela; Ready, Derren; Eltringham, Gary; Richards, Jade; Vipond, Richard; Lukaszewski, Roman; Payne, Phillippa M; Aarons, Emma; Simpson, Andrew J; Hutley, Emma J; Brooks, Tim

    2016-01-01

    Rapid Ebola virus (EBOV) detection is crucial for appropriate patient management and care. The performance of the FilmArray BioThreat-E test (v2.5) using whole-blood samples was evaluated in Sierra Leone and the United Kingdom and was compared with results generated by a real-time Ebola Zaire PCR reference method. Samples were tested in diagnostic laboratories upon availability, included successive samples from individual patients, and were heat treated to facilitate EBOV inactivation prior to PCR. The BioThreat-E test had a sensitivity of 84% (confidence interval [CI], 64% to 95%) and a specificity of 89% (CI, 73% to 97%) in Sierra Leone (n = 60; 44 patients) and a sensitivity of 75% (CI, 19% to 99%) and a specificity of 100% (CI, 97% to 100%) in the United Kingdom (n = 108; 70 patients) compared to the reference real-time PCR. Statistical analysis (Fisher's exact test) indicated there was no significant difference between the methods at the 99% confidence level in either country. In 9 discrepant results (5 real-time PCR positives and BioThreat-E test negatives and 4 real-time PCR negatives and BioThreat-E test positives), the majority (n = 8) were obtained from samples with an observed or probable low viral load. The FilmArray BioThreat-E test (v2.5) therefore provides an attractive option for laboratories (either in austere field settings or in countries with an advanced technological infrastructure) which do not routinely offer an EBOV diagnostic capability.

  6. The Dora Lange Archive

    DEFF Research Database (Denmark)

    Holm, Isak Winkel; Bjering, Jens Christian Borrebye

    2016-01-01

    The first season of Nic Pizzolatto's True Detective (2014) is not only a show about a murder, but also a show about how to gather and organize information in an archive. Having identified two archival problems—its temporal and topical extension—the article turns to Jacques Derrida's Archive Fever......: a Freudian Impression in order to explain how the archiving problems of the crime investigation are, in fact, intrinsic to any archiving practice. Lastly, the article addresses the political significance of the show's archiving problems by help of Derrida's text on the American Constitution and of Hardt...

  7. Update History of This Database - tRNADB-CE | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us tRNADB-CE Update... History of This Database Date Update contents 2014/08/25 Contact address, Database maintena...nce site, URL of the original website and Whole data download are changed. 2012/07/23 - Data Updated The data is update... sample (ENV) from GenBank: 134 Environmental sample (ENV) from Sequence Read Archive: 87 2011/08/25 License is update... This Database Database Description Download License Update History of This Database Site Policy | Contact Us Update History of This Database - tRNADB-CE | LSDB Archive ...

  8. Archives Library Information Center

    Data.gov (United States)

    National Archives and Records Administration — ALIC is an online library catalog of books, periodicals, and other materials contained in Archives I and II and book collections located in other facilities.

  9. Mandated data archiving greatly improves access to research data

    OpenAIRE

    Vines, Timothy H; Andrew, Rose L.; Bock, Dan G; Franklin, Michelle T.; Gilbert, Kimberly J.; Kane, Nolan C.; Moore, Jean-Sébastien; Brook T. Moyers; Renaut, Sébastien; Rennison, Diana J.; Veen, Thor; Yeaman, Sam

    2013-01-01

    The data underlying scientific papers should be accessible to researchers both now and in the future, but how best can we ensure that these data are available? Here we examine the effectiveness of four approaches to data archiving: no stated archiving policy, recommending (but not requiring) archiving, and two versions of mandating data deposition at acceptance. We control for differences between data types by trying to obtain data from papers that use a single, widespread population genetic ...

  10. Salton Sea Scientific Drilling Project Archival Reference, Final Draft

    Energy Technology Data Exchange (ETDEWEB)

    None

    1991-03-13

    This report provides an archival reference to the scientific information and other pertinent documents and materials associated with the Salton Sea Scientific Drilling Project (SSDP). This archiving process ensures that valuable technical data and information obtained during the life of the project can be retrieved, organized and maintained as a historical record for future reference. This paper describes the background of the project and the process used for archiving the materials. [DJE-2005

  11. Calorimeter Process Variable Archiving

    Energy Technology Data Exchange (ETDEWEB)

    Huffman, David; /Fermilab

    2002-01-14

    These steps were taken to maintain weekly archives: (1) Friday morning you stop the archiver and wait for it to finish writing data (the lock file will be removed from the directory); (2) move the current archive information to a PC via FTP; (3) remove all previous archive information in the previous directory; (4) move the current archive into the previous directory; (5) start a new archive; (6) burn a CDROM of the archive; and (7) copy the current archive to a specific directory. There are 2 ways to check if the Calorimeter Archiver is running, either through the WEB based front end or directly from a command line. Once the archiver is running it can be monitored from a WEB page. This only works with a browser launched from the online machine running the archiver. Each time the browser is reloaded there should be an update reported in the last write check field. You might have to wait a few minutes to see the update. Calorimetry currently takes readings every (300 sec.) 5 minutes. The second method to verify the archiver is running is to issue a command from a Linux cluster machine.

  12. Opening the archives for state of the art tumour genetic research: sample processing for array-CGH using decalcified, formalin-fixed, paraffin-embedded tissue-derived DNA samples

    OpenAIRE

    Bovee Judith VMG; Hogendoorn Pancras CW; Meijer Danielle; Verbeke Sofie LJ; de Jong Danielle; Szuhai Károly

    2011-01-01

    Abstract Background Molecular genetic studies on rare tumour entities, such as bone tumours, often require the use of decalcified, formalin-fixed, paraffin-embedded tissue (dFFPE) samples. Regardless of which decalcification procedure is used, this introduces a vast breakdown of DNA that precludes the possibility of further molecular genetic testing. We set out to establish a robust protocol that would overcome these intrinsic hurdles for bone tumour research. Findings The goal of our study w...

  13. Your Laptop to the Rescue: Using the Child Language Data Exchange System Archive and CLAN Utilities to Improve Child Language Sample Analysis.

    Science.gov (United States)

    Ratner, Nan Bernstein; MacWhinney, Brian

    2016-05-01

    In this article, we review the advantages of language sample analysis (LSA) and explain how clinicians can make the process of LSA faster, easier, more accurate, and more insightful than LSA done "by hand" by using free, available software programs such as Computerized Language Analysis (CLAN). We demonstrate the utility of CLAN analysis in studying the expressive language of a very large cohort of 24-month-old toddlers tracked in a recent longitudinal study; toddlers in particular are the most likely group to receive LSA by clinicians, but existing reference "norms" for this population are based on fairly small cohorts of children. Finally, we demonstrate how a CLAN utility such as KidEval can now extract potential normative data from the very large number of corpora now available for English and other languages at the Child Language Data Exchange System project site. Most of the LSA measures that we studied appear to show developmental profiles suggesting that they may be of specifically higher value for children at certain ages, because they do not show an even developmental trajectory from 2 to 7 years of age. PMID:27111268

  14. Prenatal polychlorinated biphenyl exposure is associated with decreased gestational length but not birth weight: archived samples from the Child Health and Development Studies pregnancy cohort

    Directory of Open Access Journals (Sweden)

    Kezios Katrina L

    2012-07-01

    Full Text Available Abstract Background Polychlorinated biphenyls (PCBs, known endocrine disruptors, were banned in 1979 but persist in the environment. Previous studies are inconsistent regarding prenatal exposure to PCBs and pregnancy outcomes. We investigated associations between prenatal exposure to PCBs and gestational length and birth weight. Methods In a sample of 600 infants (born between 1960 and 1963 randomly selected from Child Health and Development Studies participants followed through adolescence we measured 11 PCB congeners in maternal post partum sera (within three days of delivery. Length of gestation was computed from the reported first day of the last menstrual period (LMP and delivery date. Linear regression was used to estimate associations between PCB exposure and gestational age and birth weight, adjusting for potential confounders. PCBs were grouped according to hypothesized biological action (1b (sum of weak phenobarbital inducers, 2b (sum of limited dioxin activity, and 3 (sum of CYP1A and CYP2b inducers or degree of ortho- substitution (mono, di, tri. Secondary analyses examined associations between total PCB exposure and exposure to individual congeners. Results Each unit increase in mono-ortho substituted PCBs was associated with a 0.30 week decrease (95% confidence interval (CI -0.59, -0.016, corresponding to a 2.1 (95% CI −4.13, -0.11 day decrease in length of gestation. Similar associations were estimated for di-ortho substituted PCBs, (1.4 day decrease; (95% CI −2.9, 0.1 and group 3 PCBs (0.84 day decrease; (95% CI −1.8, 0.11. We found similar associations in congener specific analyses and for the sum of congeners. Conclusions Our study provides new evidence that PCB exposure shortens length of gestation in humans. This may have public health implications for population exposures.

  15. Detection of microRNAs in archival cytology urine smears.

    Science.gov (United States)

    Simonato, Francesca; Ventura, Laura; Sartori, Nicola; Cappellesso, Rocco; Fassan, Matteo; Busund, Lill-Tove; Fassina, Ambrogio

    2013-01-01

    MicroRNAs' dysregulation and profiling have been demonstrated to be clinically relevant in urothelial carcinoma (UC). Urine cytology is commonly used as the mainstay non-invasive test for secondary prevention and follow-up of UC patients. Ancillary tools are needed to support cytopathologists in the diagnosis of low-grade UC. The feasibility and reliability of microRNAs profiling by qRT-PCR analysis (miR-145 and miR-205) in archival routine urine cytology smears (affected by fixation/staining [Papanicolau] and room temperature storage) was tested in a series of 15 non-neoplastic and 10 UC urine specimens. Only samples with >5,000 urothelial cells and with <50% of inflammatory cells/red blood cells clusters were considered. Overall, a satisfactory amount of total RNA was obtained from all the considered samples (mean 1.27±1.43 µg, range 0.06-4.60 µg). Twenty nanograms of total RNA have been calculated to be the minimal total RNA concentration for reliable and reproducible miRNAs expression profiling analysis of archival cytological smears (slope= -3.4084; R-squared=0.99; efficiency=1.94). miR-145 and miR-205 were significantly downregulated in UC samples in comparison to non-tumor controls. These findings demonstrate that urine archival cytology smears are suitable for obtaining high-quality RNA to be used in microRNAs expression profiling. Further studies should investigate if miRNAs profiling can be successfully translated into clinical practice as diagnostic or prognostic markers.

  16. Detection of microRNAs in archival cytology urine smears.

    Directory of Open Access Journals (Sweden)

    Francesca Simonato

    Full Text Available MicroRNAs' dysregulation and profiling have been demonstrated to be clinically relevant in urothelial carcinoma (UC. Urine cytology is commonly used as the mainstay non-invasive test for secondary prevention and follow-up of UC patients. Ancillary tools are needed to support cytopathologists in the diagnosis of low-grade UC. The feasibility and reliability of microRNAs profiling by qRT-PCR analysis (miR-145 and miR-205 in archival routine urine cytology smears (affected by fixation/staining [Papanicolau] and room temperature storage was tested in a series of 15 non-neoplastic and 10 UC urine specimens. Only samples with >5,000 urothelial cells and with <50% of inflammatory cells/red blood cells clusters were considered. Overall, a satisfactory amount of total RNA was obtained from all the considered samples (mean 1.27±1.43 µg, range 0.06-4.60 µg. Twenty nanograms of total RNA have been calculated to be the minimal total RNA concentration for reliable and reproducible miRNAs expression profiling analysis of archival cytological smears (slope= -3.4084; R-squared=0.99; efficiency=1.94. miR-145 and miR-205 were significantly downregulated in UC samples in comparison to non-tumor controls. These findings demonstrate that urine archival cytology smears are suitable for obtaining high-quality RNA to be used in microRNAs expression profiling. Further studies should investigate if miRNAs profiling can be successfully translated into clinical practice as diagnostic or prognostic markers.

  17. Subject Access Points in the MARC Record and Archival Finding Aid: Enough or Too Many?

    Science.gov (United States)

    Cox, Elizabeth; Czechowski, Leslie

    2007-01-01

    In this research project, the authors set out to discover the current practice in both the archival and cataloging worlds for usage of access points in descriptive records and to learn how archival descriptive practices fit into long-established library cataloging procedures and practices. A sample of archival finding aids and MARC records at 123…

  18. Introduction: Consider the Archive.

    Science.gov (United States)

    Yale, Elizabeth

    2016-03-01

    In recent years, historians of archives have paid increasingly careful attention to the development of state, colonial, religious, and corporate archives in the early modern period, arguing that power (of various kinds) was mediated and extended through material writing practices in and around archives. The history of early modern science, likewise, has tracked the production of scientific knowledge through the inscription and circulation of written records within and between laboratories, libraries, homes, and public spaces, such as coffeehouses and bookshops. This Focus section interrogates these two bodies of scholarship against each other. The contributors ask how archival digitization is transforming historical practice; how awareness of archival histories can help us to reconceptualize our work as historians of science; how an archive's layered purposes, built up over centuries of record keeping, can shape the historical narratives we write; and how scientific knowledge emerging from archives gained authority and authenticity. PMID:27197412

  19. Mandated data archiving greatly improves access to research data

    CERN Document Server

    Vines, Timothy H; Bock, Dan G; Franklin, Michelle T; Gilbert, Kimberly J; Kane, Nolan C; Moore, Jean-Sébastien; Moyers, Brook T; Renaut, Sébastien; Rennison, Diana J; Veen, Thor; Yeaman, Sam

    2013-01-01

    The data underlying scientific papers should be accessible to researchers both now and in the future, but how best can we ensure that these data are available? Here we examine the effectiveness of four approaches to data archiving: no stated archiving policy, recommending (but not requiring) archiving, and two versions of mandating data deposition at acceptance. We control for differences between data types by trying to obtain data from papers that use a single, widespread population genetic analysis, STRUCTURE. At one extreme, we found that mandated data archiving policies that require the inclusion of a data availability statement in the manuscript improve the odds of finding the data online almost a thousand-fold compared to having no policy. However, archiving rates at journals with less stringent policies were only very slightly higher than those with no policy at all. At one extreme, we found that mandated data archiving policies that require the inclusion of a data availability statement in the manuscr...

  20. A method to evaluate genome-wide methylation in archival formalin-fixed, paraffin-embedded ovarian epithelial cells.

    Directory of Open Access Journals (Sweden)

    Qiling Li

    Full Text Available BACKGROUND: The use of DNA from archival formalin and paraffin embedded (FFPE tissue for genetic and epigenetic analyses may be problematic, since the DNA is often degraded and only limited amounts may be available. Thus, it is currently not known whether genome-wide methylation can be reliably assessed in DNA from archival FFPE tissue. METHODOLOGY/PRINCIPAL FINDINGS: Ovarian tissues, which were obtained and formalin-fixed and paraffin-embedded in either 1999 or 2011, were sectioned and stained with hematoxylin-eosin (H&E.Epithelial cells were captured by laser micro dissection, and their DNA subjected to whole genomic bisulfite conversion, whole genomic polymerase chain reaction (PCR amplification, and purification. Sequencing and software analyses were performed to identify the extent of genomic methylation. We observed that 31.7% of sequence reads from the DNA in the 1999 archival FFPE tissue, and 70.6% of the reads from the 2011 sample, could be matched with the genome. Methylation rates of CpG on the Watson and Crick strands were 32.2% and 45.5%, respectively, in the 1999 sample, and 65.1% and 42.7% in the 2011 sample. CONCLUSIONS/SIGNIFICANCE: We have developed an efficient method that allows DNA methylation to be assessed in archival FFPE tissue samples.

  1. DCC Briefing Paper: Database archiving

    OpenAIRE

    Müller, Heiko

    2009-01-01

    In a computational context, data archiving refers to the storage of electronic documents, data sets, multimedia files, and so on, for a defined period of time. Database archiving is usually seen as a subset of data archiving. Database archiving focuses on archiving data that are maintained under the control of a database management system and structured under a database schema, e.g., a relational database. The primary goal of database archiving is to maintain access to data in case it is late...

  2. A monotonic archive for pareto-coevolution.

    Science.gov (United States)

    de Jong, Edwin D

    2007-01-01

    Coevolution has already produced promising results, but its dynamic evaluation can lead to a variety of problems that prevent most algorithms from progressing monotonically. An important open question therefore is how progress towards a chosen solution concept can be achieved. A general solution concept for coevolution is obtained by viewing opponents or tests as objectives. In this setup known as Pareto-coevolution, the desired solution is the Pareto-optimal set. We present an archive that guarantees monotonicity for this solution concept. The algorithm is called the Incremental Pareto-Coevolution Archive (IPCA), and is based on Evolutionary Multi-Objective Optimization (EMOO). By virtue of its monotonicity, IPCA avoids regress even when combined with a highly explorative generator. This capacity is demonstrated on a challenging test problem requiring both exploration and reliability. IPCA maintains a highly specific selection of tests, but the size of the test archive nonetheless grows unboundedly. We therefore furthermore investigate how archive sizes may be limited while still providing approximate reliability. The LAyered Pareto-Coevolution Archive (LAPCA) maintains a limited number of layers of candidate solutions and tests, and thereby permits a trade-off between archive size and reliability. The algorithm is compared in experiments, and found to be more efficient than IPCA. The work demonstrates how the approximation of a monotonic algorithm can lead to algorithms that are sufficiently reliable in practice while offering better efficiency.

  3. Data Archive Project

    OpenAIRE

    Kemppainen, Timo-Pekka

    2015-01-01

    In the modern world, digital data archiving and accessing is an issue that needs tailored solutions. This thesis gives one example of designing and implementing a data archiving solution using time tested technologies. Helsinki Metropolia University of Applied Sciences had a need to archive their contracts in digital format instead of using old file cabinets. Specifications were created together with the customer at the start of the project. The project was to produce a complete product fo...

  4. Meteorological Archival Facility

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: The Bergen Data Center (BDC) provides data archival capability for meteorological and oceanographic data. DESCRIPTION: The BDC operates as a resource for...

  5. Nearline Web Archiving

    OpenAIRE

    Xie, Zhiwu; Nayyar, Krati; Fox, Edward A

    2016-01-01

    In this paper, we propose a modified approach to real­time transactional web archiving. It leverages the web caching infrastructure that is already prevalent on web servers. Instead of archiving web content at HTTP transaction time, in our approach the archiving happens when the cached copy expires and is about to be expunged. Before the deletion, all expired cache copies are combined and then sent to the web archive in small batches. Since the cache is purged at much lower frequency than HTT...

  6. The AMBRE Project: Stellar Parameterisation of the ESO:UVES archived spectra

    CERN Document Server

    Worley, C C; Recio-Blanco, A; Hill, V; Bijaoui, A

    2016-01-01

    The AMBRE Project is a collaboration between the European Southern Observatory (ESO) and the Observatoire de la Cote d'Azur (OCA) that has been established in order to carry out the determination of stellar atmospheric parameters for the archived spectra of four ESO spectrographs. The analysis of the UVES archived spectra for their stellar parameters has been completed in the third phase of the AMBRE Project. From the complete ESO:UVES archive dataset that was received covering the period 2000 to 2010, 51921 spectra for the six standard setups were analysed. The AMBRE analysis pipeline uses the stellar parameterisation algorithm MATISSE to obtain the stellar atmospheric parameters. The synthetic grid is currently constrained to FGKM stars only. Stellar atmospheric parameters are reported for 12,403 of the 51,921 UVES archived spectra analysed in AMBRE:UVES. This equates to ~23.9% of the sample and ~3,708 stars. Effective temperature, surface gravity, metallicity and alpha element to iron ratio abundances are ...

  7. The Gaia Archive at ESAC: a VO-inside archive

    Science.gov (United States)

    Gonzalez-Nunez, J.

    2015-12-01

    The ESDC (ESAC Science Data Center) is one of the active members of the IVOA (International Virtual Observatory Alliance) that have defined a set of standards, libraries and concepts that allows to create flexible,scalable and interoperable architectures on the data archives development. In the case of astronomy science that involves the use of big catalogues, as in Gaia or Euclid, TAP, UWS and VOSpace standards can be used to create an architecture that allows the explotation of this valuable data from the community. Also, new challenges arise like the implementation of the new paradigm "move code close to the data", what can be partially obtained by the extension of the protocols (TAP+, UWS+, etc) or the languages (ADQL). We explain how we have used VO standards and libraries for the Gaia Archive that, not only have producing an open and interoperable archive but, also, minimizing the developement on certain areas. Also we will explain how we have extended these protocols and the future plans.

  8. The GESIS Data Archive for the Social Sciences: A Widely Recognised Data Archive on its Way

    Directory of Open Access Journals (Sweden)

    Natascha Schumann

    2013-11-01

    Full Text Available This paper describes initial experiences in evaluating an established data archive with a long-standing commitment to preservation and dissemination of social science research data against recently formulated standards for trustworthy digital archives. As stakeholders need to be sure that the data they produce, use or fund is treated according to common standards, the GESIS Data Archive decided to start a process of audit and certification within the European Framework of Certification and Audit, starting with the Data Seal of Approval (DSA. This paper gives an overview of workflows within the archive and illustrates some of the steps necessary to obtain the DSA as well as to optimize some of its services. Finally, a short appraisal of the method of the DSA is made.

  9. Inventing the Archive

    DEFF Research Database (Denmark)

    Eskildsen, Kasper Risbjerg

    2013-01-01

    This article investigates the emergence of the archive as the primary venue for the production of historical knowledge in the 19th century. The turn to archival research, the article argues, may be considered as a response to the discussions about the problems of testimony that dominated 18th- an...

  10. Online archives of Science

    CERN Multimedia

    GS Department

    2009-01-01

    CERN Library has enabled till 26th June 2009 a trial access to the online archives of Science, starting in 1880. You are welcome to test this resource at http://www.sciencemag.org/archive/ Please contact mailto:Anne.Gentil-Beccot@cern.ch, for any feedback to share.

  11. In the Archives: A User's Perspective

    Directory of Open Access Journals (Sweden)

    Ellen Schrecker

    2016-07-01

    Full Text Available Radical researchers need to use archives, but can often encounter problems with obtaining access to them. Currently, most of those problems are the result of cut-backs in the financing of non-profit instiutions and public higher education.

  12. An automated archival VLA transients survey

    NARCIS (Netherlands)

    Bell, M.E.; Fender, R.P.; Swinbank, J.; Miller-Jones, J.C.A.; Law, C.J.; Scheers, L.H.A.; Spreeuw, H.; Wise, M.W.; Stappers, B.W.; Wijers, R.A.M.J.; Hessels, J.W.T.; Masters, J.

    2011-01-01

    In this paper we present the results of a survey for radio transients using data obtained from the Very Large Array archive. We have reduced, using a pipeline procedure, 5037 observations of the most common pointings - i.e. the calibrator fields. These fields typically contain a relatively bright po

  13. Metadata and Data Management for the Keck Observatory Archive

    CERN Document Server

    Tran, H D; Goodrich, R W; Mader, J A; Swain, M; Laity, A C; Kong, M; Gelino, C R; Berriman, G B

    2014-01-01

    A collaboration between the W. M. Keck Observatory (WMKO) in Hawaii and the NASA Exoplanet Science Institute (NExScI) in California, the Keck Observatory Archive (KOA) was commissioned in 2004 to archive observing data from WMKO, which operates two classically scheduled 10 m ground-based telescopes. The observing data from Keck is not suitable for direct ingestion into the archive since the metadata contained in the original FITS headers lack the information necessary for proper archiving. Coupled with different standards among instrument builders and the heterogeneous nature of the data inherent in classical observing, in which observers have complete control of the instruments and their observations, the data pose a number of technical challenges for KOA. We describe the methodologies and tools that we have developed to successfully address these difficulties, adding content to the FITS headers and "retrofitting" the metadata in order to support archiving Keck data, especially those obtained before the arch...

  14. Archiving the Relaxed Consistency Web

    OpenAIRE

    Xie, Zhiwu; Van de Sompel, Herbert; Liu, Jinyang; Van Reenen, Johann; Jordan, Ramiro

    2013-01-01

    The historical, cultural, and intellectual importance of archiving the web has been widely recognized. Today, all countries with high Internet penetration rate have established high-profile archiving initiatives to crawl and archive the fast-disappearing web content for long-term use. As web technologies evolve, established web archiving techniques face challenges. This paper focuses on the potential impact of the relaxed consistency web design on crawler driven web archiving. Relaxed consist...

  15. National Satellite Land Remote Sensing Data Archive

    Science.gov (United States)

    Faundeen, John L.; Kelly, Francis P.; Holm, Thomas M.; Nolt, Jenna E.

    2013-01-01

    The National Satellite Land Remote Sensing Data Archive (NSLRSDA) resides at the U.S. Geological Survey's (USGS) Earth Resources Observation and Science (EROS) Center. Through the Land Remote Sensing Policy Act of 1992, the U.S. Congress directed the Department of the Interior (DOI) to establish a permanent Government archive containing satellite remote sensing data of the Earth's land surface and to make this data easily accessible and readily available. This unique DOI/USGS archive provides a comprehensive, permanent, and impartial observational record of the planet's land surface obtained throughout more than five decades of satellite remote sensing. Satellite-derived data and information products are primary sources used to detect and understand changes such as deforestation, desertification, agricultural crop vigor, water quality, invasive plant species, and certain natural hazards such as flood extent and wildfire scars.

  16. Chemical structures and thermal properties of polyesters obtained from different samples of bio diesel epoxidized; Estruturas quimicas e propriedades termicas de poliesteres obtidos a partir de diferentes tipos de biodiesel epoxidado

    Energy Technology Data Exchange (ETDEWEB)

    Samios, Dimitrios; Reiznautt, Quelen B.; Nicolau, Aline; Martini, Denise D.; Chagas, Arthur L. das, E-mail: dsamios@iq.ufrgs.b [Universidade Federal do Rio Grande do Sul (IQ/UFRGS), Porto Alegre, RS (Brazil). Inst. de Quimica. Lab. de Instrumentacao e Dinamica Molecular

    2009-07-01

    In this work new structures from oligo esters and polyesters from different oils (olive oil, sunflower oil and linseed oil) were synthesized and characterized. Oligo esters and polyesters were synthesized from the reaction of fatty acid methyl epoxy-esters, obtained from different oils, with cis-1,2-cyclohexanedicarboxylic anhydride in the presence of triethylamine (TEA). Different amounts of the resin 1,4-butanediol diglycidyl ether (BDGE) were added in order to increase the capacity of crosslinking. The molar ratio of BDGE used in system was between 0 and 0.066. The intermediate structures, as well as the oligo esters and polyesters produced, were analyzed by using Fourier Transform Infrared Spectroscopy and Nuclear Magnetic Resonance ({sup 1}H - NMR). The thermal behavior of the products was realized through differential scanning calorimetry and Thermogravimetric analyses. The presence of BDGE in the materials chains increases the bonding capacity resulting in a higher molecular weight material which presents good thermal stability. (author)

  17. Complexity of nearshore strontium-to-calcium ratio variability in a core sample of the massive coral Siderastrea siderea obtained in Coral Bay, St. John, U.S. Virgin Islands

    Science.gov (United States)

    Reich, Christopher D.; Kuffner, Ilsa B.; Hickey, T. Don; Morrison, Jennifer M.; Flannery, Jennifer A.

    2013-01-01

    Strontium-to-calcium ratios (Sr/Ca) were measured on the skeletal matrix of a core sample from a colony of the massive coral Siderastrea siderea collected in Coral Bay, St. John, U.S. Virgin Islands. Strontium and calcium are incorporated into the coral skeleton during the precipitation of aragonite by the coral polyps and their ratio is highly temperature dependent. The robustness of this temperature dependence makes Sr/Ca a reliable proxy for sea surface temperature (SST). Details presented from the St. John S. siderea core indicate that terrestrial inputs of sediment and freshwater can disrupt the chemical balance and subsequently complicate the utility of Sr/Ca in reconstructing historical SST. An approximately 44-year-long record of Sr/Ca shows that an annual SST signal is recorded but with an increasing Sr/Ca trend from 1980 to present, which is likely the result of runoff from the mountainous terrain of St. John. The overwhelming influence of the terrestrial fingerprint on local seawater chemistry makes utilizing Sr/Ca as a SST proxy in nearshore environments very difficult.

  18. Police Incident Blotter (Archive)

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — The Police Blotter Archive contains crime incident data after it has been validated and processed to meet Uniform Crime Reporting (UCR) standards, published on a...

  19. Emergency_RDS_ARCHIVE

    Data.gov (United States)

    Vermont Center for Geographic Information — Archive of past 9-1-1 data releases at rough yearly intervals chosen from availbable datasets from 1999 to present. For a historical overview on the genesis of this...

  20. Mariner 10 Image Archive

    Data.gov (United States)

    National Aeronautics and Space Administration — The Mariner 10 Image Archive includes tools to view shaded relief maps of the surface of Mercury, a 3D globe, and all images acquired by NASA's Mariner 10 mission.

  1. NASA's Astrophysics Data Archives

    Science.gov (United States)

    Hasan, H.; Hanisch, R.; Bredekamp, J.

    2000-09-01

    The NASA Office of Space Science has established a series of archival centers where science data acquired through its space science missions is deposited. The availability of high quality data to the general public through these open archives enables the maximization of science return of the flight missions. The Astrophysics Data Centers Coordinating Council, an informal collaboration of archival centers, coordinates data from five archival centers distiguished primarily by the wavelength range of the data deposited there. Data are available in FITS format. An overview of NASA's data centers and services is presented in this paper. A standard front-end modifyer called `Astrowbrowse' is described. Other catalog browsers and tools include WISARD and AMASE supported by the National Space Scince Data Center, as well as ISAIA, a follow on to Astrobrowse.

  2. Full Tolerant Archiving System

    Science.gov (United States)

    Knapic, C.; Molinaro, M.; Smareglia, R.

    2013-10-01

    The archiving system at the Italian center for Astronomical Archives (IA2) manages data from external sources like telescopes, observatories, or surveys and handles them in order to guarantee preservation, dissemination, and reliability, in most cases in a Virtual Observatory (VO) compliant manner. A metadata model dynamic constructor and a data archive manager are new concepts aimed at automatizing the management of different astronomical data sources in a fault tolerant environment. The goal is a full tolerant archiving system, nevertheless complicated by the presence of various and time changing data models, file formats (FITS, HDF5, ROOT, PDS, etc.) and metadata content, even inside the same project. To avoid this unpleasant scenario a novel approach is proposed in order to guarantee data ingestion, backward compatibility, and information preservation.

  3. Sequence Read Archive (SRA)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sequence Read Archive (SRA) stores raw sequencing data from the next generation of sequencing platforms including Roche 454 GS System®, Illumina Genome...

  4. Download - Plabrain DB | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Plabrain...escription Download License Update History of This Database Site Policy | Contact Us Download - Plabrain DB | LSDB Archive ...

  5. License - RGP gmap | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP gmap...icense Update History of This Database Site Policy | Contact Us License - RGP gmap | LSDB Archive ...

  6. License - RGP physicalmap | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP physicalma...e Database Description Download License Update History of This Database Site Policy | Contact Us License - RGP physicalmap | LSDB Archive ...

  7. PREIMS - AT Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AT Atlas...n Download License Update History of This Database Site Policy | Contact Us PREIMS - AT Atlas | LSDB Archive ...

  8. Main - AT Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AT Atlas...wnload License Update History of This Database Site Policy | Contact Us Main - AT Atlas | LSDB Archive ...

  9. Protein - AT Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AT Atlas...base Description Download License Update History of This Database Site Policy | Contact Us Protein - AT Atlas | LSDB Archive ...

  10. Download - AT Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AT Atlas...base Description Download License Update History of This Database Site Policy | Contact Us Download - AT Atlas | LSDB Archive ...

  11. Main - TP Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us TP Atlas...load License Update History of This Database Site Policy | Contact Us Main - TP Atlas | LSDB Archive ...

  12. Post-mortem tissue biopsies obtained at minimally invasive autopsy: an RNA-quality analysis.

    Directory of Open Access Journals (Sweden)

    Anita van der Linden

    Full Text Available Bereaved relatives often refuse to give consent for post-mortem investigation of deceased cancer patients, mainly because of the mutilation due to conventional autopsy (CA. Minimally invasive autopsy (MIA may be a more acceptable alternative and, if implemented in clinical practice, creates an opportunity to more often obtain post-mortem tissue samples of (recurred primary tumors and metastases for molecular research. As a measure for tissue quality for molecular studies, we hereby present a feasibility study, comparing the RNA quality of MIA and CA samples, and fresh frozen samples as reference.Tissue samples of heart, liver and kidney were prospectively collected from 24 MIAs followed by CA, and compared to corresponding archival fresh frozen tissue. After RNA isolation and RT-qPCR, RNA integrity numbers (RIN and GAPDH expression (six amplicon sizes ranging from 71 to 530 base pairs were measured. RIN values and GAPDH Cq values were analyzed and compared between all sample groups and post-mortem intervals (PMI.RIN values in MIA samples were significantly higher than those in CA samples. GAPDH was expressed significantly higher in MIA samples than in CA samples and 530 bp PCR products could be measured in all cases. GAPDH expression was significantly lower in samples with PMI >15 hours. As expected, the samples of the fresh frozen reference standard performed best in all analyses.MIA samples showed better RNA quality than CA samples, probably due to shorter PMI. Both had lower RNA quality and expression levels than fresh frozen tissue, however, remaining GAPDH RNA was still sufficiently intact. Therefore, other highly expressed genes are most likely also detectable. Gene array analysis should be performed to gain insight into the quality of entire post-mortem genomes. Reducing PMI will further improve the feasibility of demanding molecular research on post-mortem tissues, this is most likely more feasible with MIA than CA.

  13. Main - RPSD | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ... File URL: ftp://ftp.biosciencedbc.jp/archive/rpsd/LATEST/rpsd_main_sjis.zip File... size: 120 KB File name: rpsd_main_utf8.zip File URL: ftp://ftp.biosciencedbc.jp/archive/rpsd/LATEST/rpsd_ma...icense Update History of This Database Site Policy | Contact Us Main - RPSD | LSDB Archive ...

  14. Nucleotide Sequence - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ..._db.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_ine_full_se...quence_db.zip File size: 19 MB File name: FASTA: kome_ine_full_sequence_db.fasta.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...rtio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us Nucleotide Sequence - KOME | LSDB Archive ...

  15. Training in the Archives: Archival Research as Professional Development

    Science.gov (United States)

    Buehl, Jonathan; Chute, Tamar; Fields, Anne

    2012-01-01

    This article describes the rationale and efficacy of a graduate-level teaching module providing loosely structured practice with real archives. Introducing early career scholars to archival methods changed their beliefs about knowledge, research, teaching, and their discipline(s). This case study suggests that archives can be productive training…

  16. Basical information - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ation about full-length cDNA clones Data file File name: kome_basical_information.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...base Database Description Download License Update History of This Database Site Policy | Contact Us Basical information - KOME | LSDB Archive ...

  17. Entire Sequence - RMG | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...us RAP-DB genome browser. Data file File name: rmg_seq.zip File URL: ftp://ftp.biosciencedbc.jp/archive/rmg/...n Download License Update History of This Database Site Policy | Contact Us Entire Sequence - RMG | LSDB Archive ...

  18. Genome annotations - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ....zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_genome_annotat...e Update History of This Database Site Policy | Contact Us Genome annotations - KOME | LSDB Archive ...

  19. Trimming information - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ation.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_trimming_information.zip File size: ...SEF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us Trimming information - KOME | LSDB Archive ...

  20. Product annotations - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ile name: kome_product_annotation.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...ate History of This Database Site Policy | Contact Us Product annotations - KOME | LSDB Archive ...

  1. OPS index - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ile URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_ops_index.zip File s...la SEF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us OPS index - KOME | LSDB Archive ...

  2. ORF information - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...e longest ORFs Data file File name: kome_orf_infomation.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kom...e Description Download License Update History of This Database Site Policy | Contact Us ORF information - KOME | LSDB Archive ...

  3. Reading Robin Kelsey's "Archive Style" across the Archival Divide

    Science.gov (United States)

    Schwartz, Joan M.

    2008-01-01

    This article presents the author's comments on Robin Kelsey's "Archive Style: Photographs and Illustrations for U.S. Surveys, 1850-1890," a book about government documents that happen to be visual materials. The word "archive" now has intellectual cache in the academic world, but its currency has little to do with the "real world of archives" as…

  4. Main - PLACE | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...URL: ftp://ftp.biosciencedbc.jp/archive/place/LATEST/place_main.zip File size: 48...97) Joomla SEF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us Main - PLACE | LSDB Archive ...

  5. Ten years of archival science

    NARCIS (Netherlands)

    E. Ketelaar

    2010-01-01

    This article reviews how the journal Archival Science--International Journal on Recorded Information in the first 10 years has endeavoured to be integrated, interdisciplinary, and intercultural in promoting the development of archival science as an autonomous scientific discipline.

  6. Life Sciences Data Archive (LSDA)

    Data.gov (United States)

    National Aeronautics and Space Administration — NASA's Life Sciences Data Archive (LSDA) is an active archive that provides information and data from 1961 (Mercury Project) through current flight and flight...

  7. Three dimensional characterization and archiving system

    Energy Technology Data Exchange (ETDEWEB)

    Clark, R.; Gallman, P.; Gaudreault, J.; Mosehauer, R.; Slotwinski, A.; Jarvis, G.; Griffiths, P.

    1996-12-31

    This system (3D-ICAS) is being developed as a remote system to perform rapid in situ analysis of hazardous organics and radionuclide contamination on structural materials. It is in the final phase of a 3-phase program to support Decontamination and Decommissioning (D&D) operations. Accurate physical characterization of surfaces and radioactive and organic contamination is a critical D&D task. Surface characterization includes identification of dangerous inorganic materials such as asbestos and transite. 3D-ICAS robotically conveys a multisensor probe near the surfaces to be inspected, using coherent laser radar tracking, which also provides 3D facility maps. High-speed automated organic analysis is provided by means of gas chromatograph-mass spectrometer sensor which can process a sample without contact in one minute. Volatile organics are extracted directly from contaminated surfaces without sample removal; multiple stage focusing is used for high time resolution. Additional discrimination is obtained through a final stage time-of-flight mass spectrometer. The radionuclide sensors combines {alpha}, {beta}, and {gamma} counting with energy discrimination of the {alpha} channel; this quantifies isotopes of U, Pu, Th, Tc, Np, and Am in one minute. The Molecular Vibrational Spectrometry sensor is used to characterize substrate material such as concrete, transite, wood, or asbestos; this can be used to provide estimates of the depth of contamination. The 3D-ICAS will be available for real-time monitoring immediately after each 1 to 2 minute sample period. After surface mapping, 3-D displays will be provided showing contours of detected contaminant concentrations. Permanent measurement and contaminant level archiving will be provided, assuring data integrity and allowing regulatory review before and after D&D operations.

  8. Three dimensional characterization and archiving system

    International Nuclear Information System (INIS)

    This system (3D-ICAS) is being developed as a remote system to perform rapid in situ analysis of hazardous organics and radionuclide contamination on structural materials. It is in the final phase of a 3-phase program to support Decontamination and Decommissioning (D ampersand D) operations. Accurate physical characterization of surfaces and radioactive and organic contamination is a critical D ampersand D task. Surface characterization includes identification of dangerous inorganic materials such as asbestos and transite. 3D-ICAS robotically conveys a multisensor probe near the surfaces to be inspected, using coherent laser radar tracking, which also provides 3D facility maps. High-speed automated organic analysis is provided by means of gas chromatograph-mass spectrometer sensor which can process a sample without contact in one minute. Volatile organics are extracted directly from contaminated surfaces without sample removal; multiple stage focusing is used for high time resolution. Additional discrimination is obtained through a final stage time-of-flight mass spectrometer. The radionuclide sensors combines α, β, and γ counting with energy discrimination of the α channel; this quantifies isotopes of U, Pu, Th, Tc, Np, and Am in one minute. The Molecular Vibrational Spectrometry sensor is used to characterize substrate material such as concrete, transite, wood, or asbestos; this can be used to provide estimates of the depth of contamination. The 3D-ICAS will be available for real-time monitoring immediately after each 1 to 2 minute sample period. After surface mapping, 3-D displays will be provided showing contours of detected contaminant concentrations. Permanent measurement and contaminant level archiving will be provided, assuring data integrity and allowing regulatory review before and after D ampersand D operations

  9. The Materiality of Method: The Case of the Mass Observation Archive

    OpenAIRE

    Liz Moor; Emma Uprichard

    2014-01-01

    The Mass Observation Archive presents numerous methodological issues for social researchers. The data are idiosyncratic, difficult to analyze, and the sample design is nonsystematic. Such issues seriously challenge conventional social research protocols. This article highlights a further characteristic of the archive: its unwieldy materiality. Focusing on the sensory experiences of the archive and its particular type of data, the article shows how the experience of getting ‘dirty with dataâ...

  10. 20 Years of Archive Fever

    OpenAIRE

    Atkins, Guy; Bulley, James

    2014-01-01

    "It is what is happening, right here, when a house, the Freuds' last house, becomes a museum: the passage from one institution to another." (Jacques Derrida, 'Archive Fever') Presented as a gift to the Freud Museum, Jacques Derrida's 1994 lecture 'Archive Fever' remains a compelling work for scholars and artists interested in the relationship between archives, memory, and technology. Originally titled 'The Concept of the Archive: A Freudian Impression', Derrida's deconstruction of the...

  11. Impact of cleaning before obtaining midstream urine samples from children

    DEFF Research Database (Denmark)

    Lytzen, Rebekka; Knudsen, Jenny Dahl; Ladelund, Steen;

    2014-01-01

    % to 55% (p = 0.018). No significant effect was demonstrated in 10-15-year-old girls (p = 1.0) or in boys, independent of age (p = 0.19). In both periods, 31% of paired MSUs from the same child were without any bacterial or fungal growth. CONCLUSION: Cleaning before collecting urine from girls younger...

  12. Cultivating archives: meanings and identities

    NARCIS (Netherlands)

    E. Ketelaar

    2012-01-01

    By cultivating archives through successive activations, people and communities define their identities. In these activations, the meanings of archives are constructed and reconstructed. Archives are not a static artifact imbued with the record creator’s voice alone, but a dynamic process involving a

  13. The Ethics of Archival Research

    Science.gov (United States)

    McKee, Heidi A.; Porter, James E.

    2012-01-01

    What are the key ethical issues involved in conducting archival research? Based on examination of cases and interviews with leading archival researchers in composition, this article discusses several ethical questions and offers a heuristic to guide ethical decision making. Key to this process is recognizing the person-ness of archival materials.…

  14. In through the Archive

    DEFF Research Database (Denmark)

    Breinbjerg, Morten; Fritsch, Jonas; Bertelsen, Olav W.

    In this paper we will present a project in which we have orchestrated a collective listening process framed around the notion of the echo - and the interactive listening machine Ekkomaten – to build a participatory sound archive that reflects people’s perceptions and imaginations of a local...... community in the city of Aarhus Denmark. The archive is seen as indicative to the rhythm and nature of the place revealing not only aspects of the lives that unfold, but also how people sense and interpret the local community through their auditory sensibility. During the project we have asked people of all...... in the area for many years. The sound material has been organized in an archive, whichis handled by custom build software (API), and accessible through a physical, designfictional machine called Ekkomaten. In the paper, we would like to discuss the project in relation to the following subjects: 1) Methodology...

  15. The digital archive of the International Halley Watch

    Science.gov (United States)

    Klinglesmith, D. A., III; Niedner, M. B.; Grayzeck, E.; Aronsson, M.; Newburn, R. L.; Warnock, A., III

    1992-01-01

    The International Halley Watch was established to coordinate, collect, archive, and distribute the scientific data from Comet P/Halley that would be obtained from both the ground and space. This paper describes one of the end products of that effort, namely the IHW Digital Archive. The IHW Digital Archive consists of 26 CD-ROM's containing over 32 gigabytes of data from the 9 IHW disciplines as well as data from the 5 spacecraft missions flown to comet P/Haley and P/Giacobini-Zinner. The total archive contains over 50,000 observations by 1,500 observers from at least 40 countries. The first 24 CD's, which are currently available, contain data from the 9 IHW disciplines. The two remaining CD's will have the spacecraft data and should be available within the next year. A test CD-ROM of these data has been created and is currently under review.

  16. Mandated data archiving greatly improves access to research data.

    Science.gov (United States)

    Vines, Timothy H; Andrew, Rose L; Bock, Dan G; Franklin, Michelle T; Gilbert, Kimberly J; Kane, Nolan C; Moore, Jean-Sébastien; Moyers, Brook T; Renaut, Sébastien; Rennison, Diana J; Veen, Thor; Yeaman, Sam

    2013-04-01

    The data underlying scientific papers should be accessible to researchers both now and in the future, but how best can we ensure that these data are available? Here we examine the effectiveness of four approaches to data archiving: no stated archiving policy, recommending (but not requiring) archiving, and two versions of mandating data deposition at acceptance. We control for differences between data types by trying to obtain data from papers that use a single, widespread population genetic analysis, structure. At one extreme, we found that mandated data archiving policies that require the inclusion of a data availability statement in the manuscript improve the odds of finding the data online almost 1000-fold compared to having no policy. However, archiving rates at journals with less stringent policies were only very slightly higher than those with no policy at all. We also assessed the effectiveness of asking for data directly from authors and obtained over half of the requested datasets, albeit with ∼8 d delay and some disagreement with authors. Given the long-term benefits of data accessibility to the academic community, we believe that journal-based mandatory data archiving policies and mandatory data availability statements should be more widely adopted. PMID:23288929

  17. The Digital Archive Experience

    DEFF Research Database (Denmark)

    Søndergaard, Morten

    2009-01-01

    This article, as well as the book, investigates the ways in which new digital media may enhance the experience of the art-archive. Taken as a whole, the new media is a vital component of a 'transdisciplinary' and transformative field, a cultural landscape that is changing rapidly the conditions....... The book discusses the challenges of the archive and the (art)museum in the age of digital media. It is based upon documentation from a research project, MAP - Media Art Platform, that drew upon the talents and collaboration of many institutions, artists, programmers, art historians, designers and others...

  18. Digital audiovisual archives

    CERN Document Server

    Stockinger, Peter

    2013-01-01

    Today, huge quantities of digital audiovisual resources are already available - everywhere and at any time - through Web portals, online archives and libraries, and video blogs. One central question with respect to this huge amount of audiovisual data is how they can be used in specific (social, pedagogical, etc.) contexts and what are their potential interest for target groups (communities, professionals, students, researchers, etc.).This book examines the question of the (creative) exploitation of digital audiovisual archives from a theoretical, methodological, technical and practical

  19. Digitisation of the Edo Mihevc’ Archive

    Directory of Open Access Journals (Sweden)

    Neža Čebron Lipovec

    2013-09-01

    Full Text Available EXTENDED ABSTRACT:During the most productive period of his long lasting activity, namely in 1950’s and 1970’s of the 20th century, the Slovenian architect Edo Mihevc had a great impact on the development of landscape and urban architecture, especially in the Slovenian coastal region and Istria, where a great part of urban environment is either directly or indirectly related to his work. The Regional Archive of Koper obtained the architect’s archive and made it accessible to the public by signing a donation agreement with the architect’s widow Ana Jontez Mihevc. The archive was originally stored in three buildings (at his home in Portorož and Ljubljana, in his ex-office and in the basement at the Faculty of Architecture in Ljubljana. The archive was then transmitted to the Regional Archive mainly for the sake of its preservation as it was endangered by inappropriate micro climate conditions (moist, dust in all three depots. The archive located at the Faculty of Architecture was endangered as the documents were gradually taken away without any control although they had not been catalogued yet. The archive of Edo Mihevc (1911-1985 is gaining its importance for researches in the history of architecture as well as for studies of the period between the wars and especially for studies of the post war period in Slovenia. It is of great importance to the Slovenian coastal region and Istria as the architect and his students designed the regional architectural plan for the Slovenian coast and the north-west Istria as well as urban plans for individual cities and their enlargement which were mostly carried out. Thus, the architect had a great impact on the culture and identity of the local environment. In 2010 and 2011 the Regional Archive of Koper was allocated funds for the digitisation of plans which were considered to be the most sensitive part of the architect’s archive. They were sketched on the tracing paper of different quality. As they

  20. 36 CFR 1256.98 - Can I get access to and obtain copies of USIA audiovisual records transferred to the National...

    Science.gov (United States)

    2010-07-01

    ... obtain copies of USIA audiovisual records transferred to the National Archives of the United States? 1256... United States Information Agency Audiovisual Materials in the National Archives of the United States § 1256.98 Can I get access to and obtain copies of USIA audiovisual records transferred to the...

  1. A system for obtaining an optical spectrum

    DEFF Research Database (Denmark)

    2013-01-01

    The present invention relates to a system for obtaining an optical spectrum 648 of analytes in a fluid sample, wherein a porous filter 602a, 602b is arranged so that the fluid sample may be placed onto a first region of the porous filter, and a SERS-active material 610a, 610b having a SERS...... second region. Thereby, the porous filter enables that the fluid sample is filtered so that only sufficiently small entities in the fluid sample reach the second region where they may be probed so that an optical spectrum related to the analytes in the filtered sample may be obtained. The optical system...... also comprises a light source 634, a light detector 642, and the optical analysis system is arranged for obtaining the optical spectrum 648 of the analytes adjacent to the SERS-active material 610a, 610b exclusively from the second region of the porous filter 602a, 602b....

  2. Open Archives Initiative

    OpenAIRE

    McMillan, Gail

    2004-01-01

    This presentation, which was given at the annual meeting of the Society of Scholarly Publishing on June 1, 2000, describes the Open Archives Initiative (OAI), a protocol for exchanging content between digital repositories. The presentation outlines OAI's history, core components, key stakeholders, and ongoing developments. Presented at the Society for Scholarly Publishing: June 1, 2000

  3. European Vegetation Archive (EVA)

    NARCIS (Netherlands)

    Chytrý, Milan; Hennekens, S.M.; Jiménez-Alfaro, Borja; Schaminée, J.H.J.; Haveman, Rense; Janssen, J.A.M.

    2016-01-01

    The European Vegetation Archive (EVA) is a centralized database of European vegetation plots developed by the IAVS Working Group European Vegetation Survey. It has been in development since 2012 and first made available for use in research projects in 2014. It stores copies of national and region

  4. Multi-stage sampling in genetic epidemiology.

    Science.gov (United States)

    Whittemore, A S; Halpern, J

    When data are expensive to collect, it can be cost-efficient to sample in two or more stages. In the first stage a simple random sample is drawn and then stratified according to some easily measured attribute. In each subsequent stage a random subset of previously selected units is sampled for more detailed observation, with a unit's sampling probability determined by its attributes as observed in the previous stages. These designs are useful in many medical studies; here we use them in genetic epidemiology. Two genetic studies illustrate the strengths and limitations of the approach. The first study evaluates nuclear and mitochondrial DNA in U.S. blacks. The goal is to estimate the relative contributions of white male genes and white female genes to the gene pool of African-Americans. This example shows that the Horvitz-Thompson estimators proposed for multi-stage designs can be inefficient, particularly when used with unnecessary stratification. The second example is a multi-stage study of familial prostate cancer. The goal is to gather pedigrees, blood samples and archived tissue for segregation and linkage analysis of familial prostate cancer data by first obtaining crude family data from prostate cancer cases and cancer-free controls. This second example shows the gains in efficiency from multi-stage sampling when the individual likelihood or quasilikelihood scores vary substantially across strata. PMID:9004389

  5. The list of growth image files - The Rice Growth Monitoring for The Phenotypic Functional Analysis | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ch samples. Data file File name: images_list.zip File URL: ftp://ftp.biosciencedb...c.jp/archive/agritogo-rice-phenome/LATEST/data/images_list.zip File size: 16KB Simple search URL - Data acqu

  6. Main data - RMG | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...to the public. Data file File name: rmg_main.zip File URL: ftp://ftp.biosciencedbc.jp/archive/rmg/LATEST/rmg...ch website (TogoDB) Image file File name of the image Joomla SEF URLs by Artio About This Database Database ...Description Download License Update History of This Database Site Policy | Contact Us Main data - RMG | LSDB Archive ...

  7. Main - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ..._main.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_main.zip File size: 1.3 KB Simple se... dataset on Simple search website (TogoDB) Joomla SEF URLs by Artio About This Database Database Description... Download License Update History of This Database Site Policy | Contact Us Main - KOME | LSDB Archive ...

  8. Reference - PLACE | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ... File URL: ftp://ftp.biosciencedbc.jp/archive/place/LATEST/place_reference.zip Fi...r) PubMed PubMed ID GenBank DDBJ/EMBL/GenBank accession number Joomla SEF URLs by Artio About Thi...s Database Database Description Download License Update History of This Database Site Policy | Contact Us Reference - PLACE | LSDB Archive ...

  9. Archives, Education, and Access: Learning at Interference Archive

    Directory of Open Access Journals (Sweden)

    Bonnie Gordon

    2016-07-01

    Full Text Available Archives are a tool for education and the access policy of an archive affects what kind of education takes place in its space. In this paper, we describe how Interference Archive (IA, a community archive in Brooklyn, New York, provides access through an open stacks policy and experiential learning, which allows for unique educational opportunities. These methods of providing access are intended to subvert representational power, allowing visitors, donors, and volunteers to take part in deciding how histories are told, how materials are accessed, and how the collection is re-used as a resource for learning about contemporary and historical social movements.

  10. HyperArchiver: an EPICS archiver prototype based on Hypertable

    International Nuclear Information System (INIS)

    This work started in the context of NSLS2 project at Brookhaven National Laboratory. The NSLS2 control system foresees a very high number of PV variables and has strict requirements in terms of archiving/retrieving rate: our goal was to store 10 K PV/sec and retrieve 4 K PV/sec for a group of 4 signals. The HyperArchiver is an EPICS Archiver implementation engined by Hypertable, an open source database whose internal architecture is derived from Google's Big Table. We discuss the performance of HyperArchiver and present the results of some comparative tests. (authors)

  11. The Parkes Observatory Pulsar Data Archive

    Science.gov (United States)

    Hobbs, G.; Miller, D.; Manchester, R. N.; Dempsey, J.; Chapman, J. M.; Khoo, J.; Applegate, J.; Bailes, M.; Bhat, N. D. R.; Bridle, R.; Borg, A.; Brown, A.; Burnett, C.; Camilo, F.; Cattalini, C.; Chaudhary, A.; Chen, R.; D'Amico, N.; Kedziora-Chudczer, L.; Cornwell, T.; George, R.; Hampson, G.; Hepburn, M.; Jameson, A.; Keith, M.; Kelly, T.; Kosmynin, A.; Lenc, E.; Lorimer, D.; Love, C.; Lyne, A.; McIntyre, V.; Morrissey, J.; Pienaar, M.; Reynolds, J.; Ryder, G.; Sarkissian, J.; Stevenson, A.; Treloar, A.; van Straten, W.; Whiting, M.; Wilson, G.

    2011-08-01

    The Parkes pulsar data archive currently provides access to 144044 data files obtained from observations carried out at the Parkes observatory since the year 1991. Around 105 files are from surveys of the sky, the remainder are observations of 775 individual pulsars and their corresponding calibration signals. Survey observations are included from the Parkes 70cm and the Swinburne Intermediate Latitude surveys. Individual pulsar observations are included from young pulsar timing projects, the Parkes Pulsar Timing Array and from the PULSE@Parkes outreach program. The data files and access methods are compatible with Virtual Observatory protocols. This paper describes the data currently stored in the archive and presents ways in which these data can be searched and downloaded.

  12. High Energy Astrophysics Science Archive Research Center

    Data.gov (United States)

    National Aeronautics and Space Administration — The High Energy Astrophysics Science Archive Research Center (HEASARC) is the primary archive for NASA missions dealing with extremely energetic phenomena, from...

  13. Selected Declassified Chinese Diplomatic Archives

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Since the gradual declassification of China’s diplomatic archives beginning in January 2004, a great number of confidential historical archives have been unveiled to the public, providing experts and scholars with first-hand information of great value, thus facilitating the deepening of international studies on a solid base. For the benefit of our readers, China International Studies will print on a selective basis valuable declassified archives at irregular intervals.

  14. Archived film analysis and restoration

    OpenAIRE

    Rares, A.

    2004-01-01

    The progressive degradation of current film archives poses a serious threat to the preservation of our cultural and technical heritage. Digitization and digital restoration are currently the most viable solutions for the long term preservation and high quality restoration of filmed material. They also open the path for more effective search, reuse and distribution of the archived content. This thesis covers various aspects of digital restoration applied to archived film. The main focus here l...

  15. Data Quality in Web Archiving

    OpenAIRE

    Spaniol, Marc; Denev, Dimitar; Mazeika, Arturas; Weikum, Gerhard

    2009-01-01

    Web archives preserve the history of Web sites and have high long-term value for media and business analysts. Such archives are maintained by periodically re-crawling entire Web sites of interest. From an archivist's point of view, the ideal case to ensure highest possible data quality of the archive would be to ``freeze'' the complete contents of an entire Web site during the time span of crawling and capturing the site. Of course, this is practically infea...

  16. SACS: Spitzer Archival Cluster Survey

    Science.gov (United States)

    Stern, Daniel

    Emerging from the cosmic web, galaxy clusters are the most massive gravitationally bound structures in the universe. Thought to have begun their assembly at z > 2, clusters provide insights into the growth of large-scale structure as well as the physics that drives galaxy evolution. Understanding how and when the most massive galaxies assemble their stellar mass, stop forming stars, and acquire their observed morphologies in these environments remain outstanding questions. The redshift range 1.3 imaging or from X-ray surveys. Mid-infrared (MIR) imaging with the IRAC camera on board of the Spitzer Space Telescope has changed the landscape. High-redshift clusters are easily identified in the MIR due to a combination of the unique colors of distant galaxies and a negative k-correction in the 3-5 μm range which makes such galaxies bright. Even 90-sec observations with Spitzer/IRAC, a depth which essentially all extragalactic observations in the archive achieve, is sufficient to robustly detect overdensities of L* galaxies out to z~2. Here we request funding to embark on a ambitious scientific program, the “SACS: Spitzer Archival Cluster Survey”, a comprehensive search for the most distant galaxy clusters in all Spitzer/IRAC extragalactic pointings available in the archive. With the SACS we aim to discover ~2000 of 1.3 < z < 2.5 clusters, thus provide the ultimate catalog for high-redshift MIR selected clusters: a lasting legacy for Spitzer. The study we propose will increase by more than a factor of 10 the number of high-redshift clusters discovered by all previous surveys combined, providing a high-purity, uniform sample. Matching the Spitzer/IRAC-selected clusters with data at similar and longer wavelengths available in the archive (WISE 3- 5μm, Spitzer/MIPS 24μm or Herschel/SPIRE 250μm data) we will be also able to study the dependence on the environment of star formation and AGN activity out to z~2, and to study the effect of star-forming galaxies and AGNs

  17. DNA Sampling Hook

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The DNA Sampling Hook is a significant improvement on a method of obtaining a tissue sample from a live fish in situ from an aquatic environment. A tissue sample...

  18. Les archives des bagnes

    Directory of Open Access Journals (Sweden)

    Archives Nationales d’Outre-Mer

    2008-01-01

    Full Text Available Les établissements pénitentiaires coloniaux étaient administrés par le ministère de la Marine et des Colonies, puis par le ministère des Colonies (1881, en liaison avec les ministères de la Justice et de l’Intérieur. Les archives ont par conséquent été versées aux Archives nationales d’outre-mer avec le fonds du ministère des Colonies.Le fonds représente environ 760 ml (5358 articles. Il concerne presque exclusivement les bagnes de Guyane et de Nouvelle-Calédonie et couvre la période 1852-1...

  19. Archive of Geosample Data and Information from the U.S. Geological Survey (USGS) Coastal and Marine Geology Program (CMGP) Pacific Coastal and Marine Science Center (PCMSC) Samples Repository

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. Geological Survey Coastal and Marine Geology Program (CMGP) Pacific Coastal and Marine Science Center (PCMSC) Samples Repository is a partner in the Index...

  20. Archive of Geosample Data and Information from the U.S. Geological Survey (USGS) Coastal and Marine Geology Program (CMGP) Woods Hole Coastal and Marine Science Center (WHCMSC) Samples Repository

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. Geological Survey Coastal and Marine Geology Program (CMGP) Woods Hole Coastal and Marine Science Center (WHCMSC) Samples Repository is a partner in the...

  1. Archive of Geosample Data and Information from the U.S. Geological Survey (USGS) Coastal and Marine Geology Program (CMGP) St. Petersburg Coastal and Marine Science Center (SPCMSC) Samples Repository

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. Geological Survey Coastal and Marine Geology Program (CMGP) St. Petersburg Coastal and Marine Science Center (SPCMSC) Samples Repository is a partner in...

  2. Archiving tools for EOS

    Science.gov (United States)

    Sindrilaru, Elvin-Alin; Peters, Andreas-Joachim; Duellmann, Dirk

    2015-12-01

    Archiving data to tape is a critical operation for any storage system, especially for the EOS system at CERN which holds production data for all major LHC experiments. Each collaboration has an allocated quota it can use at any given time therefore, a mechanism for archiving "stale" data is needed so that storage space is reclaimed for online analysis operations. The archiving tool that we propose for EOS aims to provide a robust client interface for moving data between EOS and CASTOR (tape backed storage system) while enforcing best practices when it comes to data integrity and verification. All data transfers are done using a third-party copy mechanism which ensures point-to- point communication between the source and destination, thus providing maximum aggregate throughput. Using ZMQ message-passing paradigm and a process-based approach enabled us to achieve optimal utilisation of the resources and a stateless architecture which can easily be tuned during operation. The modular design and the implementation done in a high-level language like Python, has enabled us to easily extended the code base to address new demands like offering full and incremental backup capabilities.

  3. The Yohkoh Legacy Archive

    Science.gov (United States)

    Acton, L. W.; Takeda, A.; McKenzie, D. E.

    2008-12-01

    Yohkoh was a Japan/US/UK mission for the study of high energy processes on the sun. Scientific operation extended from September 1991 until 14 December 2001, nearly an entire solar activity cycle. Observations included full-disk soft and hard x-ray imaging, hard x-ray spectroscopy, and high resolution flare spectroscopy in S XV, Ca XIX, Fe XXV and Fe XXVI from the Bent Crystal Spectrometer (BCS). The Yohkoh Legacy Archive (YLA) brings together all Yohkoh observational data along with extensive documentation required for a full understanding of instrumentation, mission operations, and data reduction and correction. Extensive meta-data aid the user in efficiently accessing the data base. Creation of the YLA has been the work of 8 years; the top objective has been to present the extensive Yohkoh database in a form fully usable for scientists or students who are unfamiliar with Yohkoh instrumentation. The YLA may be accessed at http://solar.physics.montana.edu/ylegacy or through the Virtual Solar Observatory (VSO), although the VSO capability is still under development. Data from the Yohkoh hard x-ray instruments and BCS are presented in flare list formats. The Soft X-ray Telescope (SXT) images are available in quantitative and movie formats. This long, uniform, archive of SXT images is especially useful for solar cycle studies as well as high resolution soft x-ray flare studies. Examples of YLA data products and research enabled by the archive will be presented.

  4. Analyzing Archival Intelligence: A Collaboration between Library Instruction and Archives

    Science.gov (United States)

    Hensley, Merinda Kaye; Murphy, Benjamin P.; Swain, Ellen D.

    2014-01-01

    Although recent archival scholarship promotes the use of primary sources for developing students' analytical research skills, few studies focus on standards or protocols for teaching or assessing archival instruction. Librarians have designed and tested standards and learning assessment strategies for library instruction, and archivists would do…

  5. The Scent of the Digital Archive. Dilemmas with Archive Digitisation

    NARCIS (Netherlands)

    C. Jeurgens

    2013-01-01

    Archival infrastructure is changing at a rapid pace as a consequence of digitisation. The effort to digitise analogue collections seems to have benefits only for researchers. Still, only a fraction of analogue archive material is currently available in digital form. This article raises some of the p

  6. Download - TP Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us TP Atlas...Ls by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us Download - TP Atlas | LSDB Archive ...

  7. Protein - TP Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us TP Atlas...EF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us Protein - TP Atlas | LSDB Archive ...

  8. License - AT Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AT Atlas...Japan . If you use data from this database, please be sure attribute this database as follows: AT Atlas © Ta...on Download License Update History of This Database Site Policy | Contact Us License - AT Atlas | LSDB Archive ...

  9. License - TP Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us TP Atlas...Japan . If you use data from this database, please be sure attribute this database as follows: TP Atlas © Ta...on Download License Update History of This Database Site Policy | Contact Us License - TP Atlas | LSDB Archive ...

  10. Experiments list - DGBY | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us DGBY Experiments... list Data detail Data name Experiments list Description of data contents Published experime...This Database Database Description Download License Update History of This Database Site Policy | Contact Us Experiments list - DGBY | LSDB Archive ...

  11. Envirion - KEGG MEDICUS | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us KEGG MEDICUS Envirio...n Data detail Data name Envirion Description of data contents KEGG ENVIRON is a collecti...oomla SEF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us Envirion - KEGG MEDICUS | LSDB Archive ...

  12. Social Media and Archives: A Survey of Archive Users

    Science.gov (United States)

    Washburn, Bruce; Eckert, Ellen; Proffitt, Merrilee

    2013-01-01

    In April and May of 2012, the Online Computer Library Center (OCLC) Research conducted a survey of users of archives to learn more about their habits and preferences. In particular, they focused on the roles that social media, recommendations, reviews, and other forms of user-contributed annotation play in archival research. OCLC surveyed faculty,…

  13. Statistical Analysis Of Tank 18F Floor Sample Results

    International Nuclear Information System (INIS)

    Representative sampling has been completed for characterization of the residual material on the floor of Tank 18F as per the statistical sampling plan developed by Shine (1). Samples from eight locations have been obtained from the tank floor and two of the samples were archived as a contingency. Six samples, referred to in this report as the current scrape samples, have been submitted to and analyzed by SRNL (2). This report contains the statistical analysis of the floor sample analytical results to determine if further data are needed to reduce uncertainty. Included are comparisons with the prior Mantis samples results (3) to determine if they can be pooled with the current scrape samples to estimate the upper 95% confidence limits (UCL95%) for concentration. Statistical analysis revealed that the Mantis and current scrape sample results are not compatible. Therefore, the Mantis sample results were not used to support the quantification of analytes in the residual material. Significant spatial variability among the current sample results was not found. Constituent concentrations were similar between the North and South hemispheres as well as between the inner and outer regions of the tank floor. The current scrape sample results from all six samples fall within their 3-sigma limits. In view of the results from numerous statistical tests, the data were pooled from all six current scrape samples. As such, an adequate sample size was provided for quantification of the residual material on the floor of Tank 18F. The uncertainty is quantified in this report by an upper 95% confidence limit (UCL95%) on each analyte concentration. The uncertainty in analyte concentration was calculated as a function of the number of samples, the average, and the standard deviation of the analytical results. The UCL95% was based entirely on the six current scrape sample results (each averaged across three analytical determinations).

  14. Statistical Analysis Of Tank 19F Floor Sample Results

    International Nuclear Information System (INIS)

    Representative sampling has been completed for characterization of the residual material on the floor of Tank 19F as per the statistical sampling plan developed by Harris and Shine. Samples from eight locations have been obtained from the tank floor and two of the samples were archived as a contingency. Six samples, referred to in this report as the current scrape samples, have been submitted to and analyzed by SRNL. This report contains the statistical analysis of the floor sample analytical results to determine if further data are needed to reduce uncertainty. Included are comparisons with the prior Mantis samples results to determine if they can be pooled with the current scrape samples to estimate the upper 95% confidence limits (UCL95%) for concentration. Statistical analysis revealed that the Mantis and current scrape sample results are not compatible. Therefore, the Mantis sample results were not used to support the quantification of analytes in the residual material. Significant spatial variability among the current scrape sample results was not found. Constituent concentrations were similar between the North and South hemispheres as well as between the inner and outer regions of the tank floor. The current scrape sample results from all six samples fall within their 3-sigma limits. In view of the results from numerous statistical tests, the data were pooled from all six current scrape samples. As such, an adequate sample size was provided for quantification of the residual material on the floor of Tank 19F. The uncertainty is quantified in this report by an UCL95% on each analyte concentration. The uncertainty in analyte concentration was calculated as a function of the number of samples, the average, and the standard deviation of the analytical results. The UCL95% was based entirely on the six current scrape sample results (each averaged across three analytical determinations).

  15. Enriching Great Britain's National Landslide Database by searching newspaper archives

    Science.gov (United States)

    Taylor, Faith E.; Malamud, Bruce D.; Freeborough, Katy; Demeritt, David

    2015-11-01

    Our understanding of where landslide hazard and impact will be greatest is largely based on our knowledge of past events. Here, we present a method to supplement existing records of landslides in Great Britain by searching an electronic archive of regional newspapers. In Great Britain, the British Geological Survey (BGS) is responsible for updating and maintaining records of landslide events and their impacts in the National Landslide Database (NLD). The NLD contains records of more than 16,500 landslide events in Great Britain. Data sources for the NLD include field surveys, academic articles, grey literature, news, public reports and, since 2012, social media. We aim to supplement the richness of the NLD by (i) identifying additional landslide events, (ii) acting as an additional source of confirmation of events existing in the NLD and (iii) adding more detail to existing database entries. This is done by systematically searching the Nexis UK digital archive of 568 regional newspapers published in the UK. In this paper, we construct a robust Boolean search criterion by experimenting with landslide terminology for four training periods. We then apply this search to all articles published in 2006 and 2012. This resulted in the addition of 111 records of landslide events to the NLD over the 2 years investigated (2006 and 2012). We also find that we were able to obtain information about landslide impact for 60-90% of landslide events identified from newspaper articles. Spatial and temporal patterns of additional landslides identified from newspaper articles are broadly in line with those existing in the NLD, confirming that the NLD is a representative sample of landsliding in Great Britain. This method could now be applied to more time periods and/or other hazards to add richness to databases and thus improve our ability to forecast future events based on records of past events.

  16. THE LA PALMA DATA ARCHIVE

    NARCIS (Netherlands)

    ZUIDERWIJK, EJ; MARTIN, R; RAIMOND, E; VANDIEPEN, GNJ

    1994-01-01

    The operation of the La Palma Data Archive is described in detail. The archive contains data taken since early 1986 with the Isaac Newton Group; 74% of the 260,000 entries are of astronomical sources. Several hundreds of queries are made on the catalog each year, resulting in approximately one reque

  17. Long term e-archiving

    OpenAIRE

    Dobratz, Susanne

    2002-01-01

    Conclusions of the breakout session "Long term e-archiving". Looking at the motto of this workshop “Gaining independence with e-Print archives and OAI” it suggests first of all that using e-Print publishing methods especially in the sense of a scholarly non-profit publishing independently from any commercial publishing house offers a unique chance to scientists.

  18. The Golosiiv on-line plate archive database, management and maintenance

    Science.gov (United States)

    Pakuliak, L.; Sergeeva, T.

    2007-08-01

    We intend to create online version of the database of the MAO NASU plate archive as VO-compatible structures in accordance with principles, developed by the International Virtual Observatory Alliance in order to make them available for world astronomical community. The online version of the log-book database is constructed by means of MySQL+PHP. Data management system provides a user with user interface, gives a capability of detailed traditional form-filling radial search of plates, obtaining some auxiliary sampling, the listing of each collection and permits to browse the detail descriptions of collections. The administrative tool allows database administrator the data correction, enhancement with new data sets and control of the integrity and consistence of the database as a whole. The VO-compatible database is currently constructing under the demands and in the accordance with principles of international data archives and has to be strongly generalized in order to provide a possibility of data mining by means of standard interfaces and to be the best fitted to the demands of WFPDB Group for databases of the plate catalogues. On-going enhancements of database toward the WFPDB bring the problem of the verification of data to the forefront, as it demands the high degree of data reliability. The process of data verification is practically endless and inseparable from data management owing to a diversity of data errors nature, that means to a variety of ploys of their identification and fixing. The current status of MAO NASU glass archive forces the activity in both directions simultaneously: the enhancement of log-book database with new sets of observational data as well as generalized database creation and the cross-identification between them. The VO-compatible version of the database is supplying with digitized data of plates obtained with MicroTek ScanMaker 9800 XL TMA. The scanning procedure is not total but is conducted selectively in the frames of special

  19. (Per)Forming Archival Research Methodologies

    Science.gov (United States)

    Gaillet, Lynee Lewis

    2012-01-01

    This article raises multiple issues associated with archival research methodologies and methods. Based on a survey of recent scholarship and interviews with experienced archival researchers, this overview of the current status of archival research both complicates traditional conceptions of archival investigation and encourages scholars to adopt…

  20. Ethics and Truth in Archival Research

    Science.gov (United States)

    Tesar, Marek

    2015-01-01

    The complexities of the ethics and truth in archival research are often unrecognised or invisible in educational research. This paper complicates the process of collecting data in the archives, as it problematises notions of ethics and truth in the archives. The archival research took place in the former Czechoslovakia and its turbulent political…

  1. Internet Art and the Archive

    Directory of Open Access Journals (Sweden)

    Karin Wagner

    2007-07-01

    Full Text Available Internet art is ephemeral by nature and several initiatives have been taken to preserve it for the future. Apart from formal archives holding art of this kind, there are also artworks which exist outside these web based institutions. In what way can they be regarded as archived? In the article, criteria are suggested which can be used to judge whether an artwork is active or archived and these criteria are applied in the analysis of twelve different artworks. Different kinds of dating are important for how the status of a work is perceived by the visitor. The concepts of explicit and implicit archiving are used to characterize archiving of Internet art, where works can be "dead" and "alive" at the same time.

  2. Archiving: The Overlooked Spreadsheet Risk

    CERN Document Server

    Lemieux, Victoria

    2008-01-01

    This paper maintains that archiving has been overlooked as a key spreadsheet internal control. The case of failed Jamaican commercial banks demonstrates how poor archiving can lead to weaknesses in spreadsheet control that contribute to operational risk. In addition, the Sarbanes-0xley Act contains a number of provisions that require tighter control over the archiving of spreadsheets. To mitigate operational risks and achieve compliance with the records-related provisions of Sarbanes-Oxley, the author argues that organisations should introduce records management programmes that provide control over the archiving of spreadsheets. At a minimum, spreadsheet archiving controls should identify and ensure compliance with retention requirements, support document production in the event of regulatory inquiries or litigation, and prevent unauthorised destruction of records.

  3. Letter to the editor: Microbial diversity in archived soils

    NARCIS (Netherlands)

    Dolfing, J.; Vos, A.; Bloem, J.; Ehlert, P.A.I.; Naumova, N.B.; Kuikman, P.J.

    2004-01-01

    A topic not covered in the recent special Section on Soils: The Final Frontier (11 June, pp. 1613-1637) is the possibility of using modern DNA-based molecular techniques to study microbial diversity in archived soil samples. Like other soil research institutes in countries such as the United Kingdom

  4. A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH.

    Science.gov (United States)

    Charwudzi, Alice; Olayemi, Edeghonghon E; Ekem, Ivy; Olopade, Olufunmilayo; Coyle, Mariann; Benneh, Amma Anima; Allotey, Emmanuel Alote

    2014-01-01

    Background. FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow and peripheral blood smear slides transported to a more equipped facility for molecular diagnosis of CML. Methods. Interphase FISH was performed on 22 archival methanol-fixed marrow (BM) and 3 peripheral blood (PB) smear slides obtained at diagnosis. The BM smears included 20 CML and 2 CMML cases diagnosed by morphology; the 3 PB smears were from 3 of the CML patients at the time of diagnosis. Six cases had known BCR-ABL fusion results at diagnosis by RQ-PCR. Full blood count reports at diagnosis were also retrieved. Result. 19 (95%) of the CML marrow smears demonstrated the BCR-ABL translocation. There was a significant correlation between the BCR-ABL transcript detected at diagnosis by RQ-PCR and that retrospectively detected by FISH from the aged BM smears at diagnosis (r = 0.870; P = 0.035). Conclusion. Archival methanol-fixed marrow and peripheral blood smears can be used to detect the BCR-ABL transcript for CML diagnosis.

  5. A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH

    Directory of Open Access Journals (Sweden)

    Alice Charwudzi

    2014-01-01

    Full Text Available Background. FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow and peripheral blood smear slides transported to a more equipped facility for molecular diagnosis of CML. Methods. Interphase FISH was performed on 22 archival methanol-fixed marrow (BM and 3 peripheral blood (PB smear slides obtained at diagnosis. The BM smears included 20 CML and 2 CMML cases diagnosed by morphology; the 3 PB smears were from 3 of the CML patients at the time of diagnosis. Six cases had known BCR-ABL fusion results at diagnosis by RQ-PCR. Full blood count reports at diagnosis were also retrieved. Result. 19 (95% of the CML marrow smears demonstrated the BCR-ABL translocation. There was a significant correlation between the BCR-ABL transcript detected at diagnosis by RQ-PCR and that retrospectively detected by FISH from the aged BM smears at diagnosis (r=0.870; P=0.035. Conclusion. Archival methanol-fixed marrow and peripheral blood smears can be used to detect the BCR-ABL transcript for CML diagnosis.

  6. Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.

    Science.gov (United States)

    de Roda Husman, A M; Snijders, P J; Stel, H V; van den Brule, A J; Meijer, C J; Walboomers, J M

    1995-08-01

    The efficiency of a freeze-thaw method, a proteinase K/Tween 20 lysis method and a guanidinium isothiocyanate/silica beads method for DNA extraction from fixed and Papanicolaou-stained cells from the cervical cancer cell line Siha was measured by beta-globin polymerase chain reaction (PCR). The GTC/silica beads method, which appeared superior, revealed a human papillomavirus (HPV) general primer-mediated PCR sensitivity of 50-500 copies of HPV 16 per sample using dilutions of fixed and stained Siha cells. Application to archival cervical smears (n = 116) revealed that the yield and size of amplifiable DNA decreases with storage time. The longer the storage time, the more repetitions of the whole procedure, including the lysis step, were required to extract sufficient amplifiable DNA. In this way, an overall beta-globin PCR positivity for 98% of the smears was reached. Further analysis revealed that a maximum size of 200 bp could be amplified from smears stored for up to 9 years. The method was validated by demonstrating by PCR the same HPV types in archival smears and corresponding cervical biopsies of cervical cancer patients. In conclusion, the GTC/silica beads method appears suitable to process archival cervical smears for HPV detection by PCR. provided that stepwise adjustments are made until beta-globin PCR positivity is obtained and primers are chosen which amplify a maximum of about 200 bp.

  7. Pathological Image Information - Open TG-GATEs Pathological Image Database | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available thological_image.zip File URL: ftp://ftp.biosciencedbc.jp/archive/open-tggates-pathological-images/LATEST/op...ts. Liver or Kidney. FILE_LOCATION FTP server path to the pathological images. CA...PTURE_NO/NO_OF_SAMPLE(n/n) The sequence number of scanned images with the total number. 1/2 means that 2 gla

  8. The Design and Operation of The Keck Observatory Archive

    CERN Document Server

    Berriman, G Bruce; Goodrich, Robert W; Holt, Jennifer; Kong, Mihseh; Laity, Anastasia C; Mader, Jeffrey A; Swain, Melanie; Tran, Hien D

    2014-01-01

    The Infrared Processing and Analysis Center (IPAC) and the W. M. Keck Observatory (WMKO) operate an archive for the Keck Observatory. At the end of 2013, KOA completed the ingestion of data from all eight active observatory instruments. KOA will continue to ingest all newly obtained observations, at an anticipated volume of 4 TB per year. The data are transmitted electronically from WMKO to IPAC for storage and curation. Access to data is governed by a data use policy, and approximately two-thirds of the data in the archive are public.

  9. ASTRONOMICAL PLATE ARCHIVES AND SUPERMASSIVE BLACK HOLE BINARIES

    Directory of Open Access Journals (Sweden)

    René Hudec

    2013-12-01

    Full Text Available The recent extensive digitisation of astronomical photographic plate archives, the development of new dedicated software and the use of powerful computers have for the first time enabled effective data mining in extensive plate databases, with wide applications in various fields of recent astrophysics. As an example, analyses of supermassive binary black holes (binary blazars require very long time intervals (50 years and more, which cannot be provided by other data sources. Examples of data obtained from data mining in plate archives are presented and briefly discussed.

  10. The AMBRE Project: Stellar parameterisation of the ESO:UVES archived spectra

    Science.gov (United States)

    Worley, C. C.; de Laverny, P.; Recio-Blanco, A.; Hill, V.; Bijaoui, A.

    2016-06-01

    Context. The AMBRE Project is a collaboration between the European Southern Observatory (ESO) and the Observatoire de la Côte d'Azur (OCA) that has been established to determine the stellar atmospheric parameters for the archived spectra of four ESO spectrographs. Aims: The analysis of the UVES archived spectra for their stellar parameters was completed in the third phase of the AMBRE Project. From the complete ESO:UVES archive dataset that was received covering the period 2000 to 2010, 51 921 spectra for the six standard setups were analysed. These correspond to approximately 8014 distinct targets (that comprise stellar and non-stellar objects) by radial coordinate search. Methods: The AMBRE analysis pipeline integrates spectral normalisation, cleaning and radial velocity correction procedures in order that the UVES spectra can then be analysed automatically with the stellar parameterisation algorithm MATISSE to obtain the stellar atmospheric parameters. The synthetic grid against which the MATISSE analysis is carried out is currently constrained to parameters of FGKM stars only. Results: Stellar atmospheric parameters are reported for 12 403 of the 51 921 UVES archived spectra analysed in AMBRE:UVES. This equates to ~23.9% of the sample and ~3708 stars. Effective temperature, surface gravity, metallicity, and alpha element to iron ratio abundances are provided for 10 212 spectra (~19.7%), while effective temperature at least is provided for the remaining 2191 spectra. Radial velocities are reported for 36 881 (~71.0%) of the analysed archive spectra. While parameters were determined for 32 306 (62.2%) spectra these parameters were not considered reliable (and thus not reported to ESO) for reasons such as very low S/N, too poor radial velocity determination, spectral features too broad for analysis, and technical issues from the reduction. Similarly the parameters of a further 7212 spectra (13.9%) were also not reported to ESO based on quality criteria and error

  11. Automatic defect detection in video archives: application to Montreux Jazz Festival digital archives

    Science.gov (United States)

    Hanhart, Philippe; Rerabek, Martin; Ivanov, Ivan; Dufaux, Alain; Jones, Caryl; Delidais, Alexandre; Ebrahimi, Touradj

    2013-09-01

    Archival of audio-visual databases has become an important discipline in multimedia. Various defects are typ- ically present in such archives. Among those, one can mention recording related defects such as interference between audio and video signals, optical related artifacts, recording and play out artifacts such as horizontal lines, and dropouts, as well as those due to digitization such as diagonal lines. An automatic or semi-automatic detection to identify such defects is useful, especially for large databases. In this paper, we propose two auto- matic algorithms for detection of horizontal and diagonal lines, as well as dropouts that are among the most typical artifacts encountered. We then evaluate the performance of these algorithms by making use of ground truth scores obtained by human subjects.

  12. Comparison of Accuracy of Whole-Exome Sequencing with Formalin-Fixed Paraffin-Embedded and Fresh Frozen Tissue Samples.

    Directory of Open Access Journals (Sweden)

    Ensel Oh

    Full Text Available Formalin fixing with paraffin embedding (FFPE has been a standard sample preparation method for decades, and archival FFPE samples are still very useful resources. Nonetheless, the use of FFPE samples in cancer genome analysis using next-generation sequencing, which is a powerful technique for the identification of genomic alterations at the nucleotide level, has been challenging due to poor DNA quality and artificial sequence alterations. In this study, we performed whole-exome sequencing of matched frozen samples and FFPE samples of tissues from 4 cancer patients and compared the next-generation sequencing data obtained from these samples. The major differences between data obtained from the 2 types of sample were the shorter insert size and artificial base alterations in the FFPE samples. A high proportion of short inserts in the FFPE samples resulted in overlapping paired reads, which could lead to overestimation of certain variants; >20% of the inserts in the FFPE samples were double sequenced. A large number of soft clipped reads was found in the sequencing data of the FFPE samples, and about 30% of total bases were soft clipped. The artificial base alterations, C>T and G>A, were observed in FFPE samples only, and the alteration rate ranged from 200 to 1,200 per 1M bases when sequencing errors were removed. Although high-confidence mutation calls in the FFPE samples were compatible to that in the frozen samples, caution should be exercised in terms of the artifacts, especially for low-confidence calls. Despite the clearly observed artifacts, archival FFPE samples can be a good resource for discovery or validation of biomarkers in cancer research based on whole-exome sequencing.

  13. The VISTA Science Archive

    CERN Document Server

    Cross, Nicholas J G; Mann, Robert G; Read, Mike A; Sutorius, Eckhard T W; Blake, Robert P; Holliman, Mark S; Hambly, Nigel C; Emerson, Jim P; Lawrence, Andrew; Noddle, Keith T

    2012-01-01

    We describe the VISTA Science Archive (VSA) and its first public release of data from five of the six VISTA Public Surveys. The VSA exists to support the VISTA Surveys through their lifecycle: the VISTA Public Survey consortia can use it during their quality control assessment of survey data products before submission to the ESO Science Archive Facility (ESO SAF); it supports their exploitation of survey data prior to its publication through the ESO SAF; and, subsequently, it provides the wider community with survey science exploitation tools that complement the data product repository functionality of the ESO SAF. This paper has been written in conjunction with the first public release of public survey data through the VSA and is designed to help its users understand the data products available and how the functionality of the VSA supports their varied science goals. We describe the design of the database and outline the database-driven curation processes that take data from nightly pipeline-processed and ca...

  14. Open archive solutions to traditional archive/library cooperation

    Directory of Open Access Journals (Sweden)

    Donatella Castelli

    2003-09-01

    Full Text Available The diffusion of Internet is changing the role of archives and libraries and it is opening a wide range of new possibilities. The new vision is that in few years it will be possible to cross-access multiple libraries, archives, museums, and data repositories. The implementation of this revolutionary vision requires the solution of a number of technical, organizational, sociological, and economical issues. Recently, the proposal of a new, low cost technical solution for open repositories of pre-print material, the Open Archives Initiative Protocol for Metadata Harvesting ( OAI-PMH, has greatly stimulated the discussion about these issues in many application frameworks. This paper overviews the status of this discussion in the library and conventional archives frameworks.

  15. 36 CFR 1256.6 - How do I obtain access to records of defunct agencies?

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false How do I obtain access to records of defunct agencies? 1256.6 Section 1256.6 Parks, Forests, and Public Property NATIONAL ARCHIVES... in function as described in Subpart B....

  16. Ultrasonic identity data storage and archival system

    International Nuclear Information System (INIS)

    Ultrasonic seals are being used to determine if an underwater stored spent fuel container has been compromised and can be used to determine if a nuclear material container has been compromised. The Seal Pattern Reader (SPAR) is a microprocessor controlled instrument which interrogates an ultrasonic seal to obtain its identity. The SPAR can compare the present identity with a previous identity, which it obtains from a magnetic bubble cassette memory. A system has been developed which allows an IAEA inspector to transfer seal information obtained at a facility by the SPAR to an IAEA-based data storage and retrieval system, using the bubble cassette memory. Likewise, magnetic bubbles can be loaded at the IAEA with seal signature data needed at a facility for comparison purposes. The archived signatures can be retrieved from the data base for relevant statistical manipulation and for plotting

  17. Use of semiconductor detector c-Si microstrip type in obtaining the digital radiographic imaging of phantoms and biological samples of mammary glands; Empleo de detector semiconductor de c-Si del tipo microbandas en la obtencion de imagenes radiograficas digitales de maniquies y muestras biologicas de mamas

    Energy Technology Data Exchange (ETDEWEB)

    Leyva, A.; Cabal, A.; Pinera, I.; Abreu, Y.; Cruz, C. M. [Centro de Estudios Avanzados y Desarrollo Nuclear, C. Habana, Cuba (Cuba); Montano, L. M.; Diaz, C. C.; Fontaine, M. [IPN, Centro de Investigaciones y Estudios Avanzados, Mexico D. F. (Mexico); Ortiz, C. M. [ISSSTE, Hospital General Tacuba, Clinica de Mama y Gineco-oncologia, Mexico D. F. (Mexico); Padilla, F. [Instituto Superior de Tecnologias y Ciencias Aplicadas, C. Habana, Cuba (Cuba); De la Mora, R. [Centro de Control Estatal de Equipos Medicos, C. Habana, Cuba (Cuba)], e-mail: aleyva@ceaden.edu.cu

    2009-07-01

    The present work synthesizes the experimental results obtained in the characterization of 64 micro strips crystalline silicon detector designed for experiments in high energies physics, with the objective of studying its possible application in advanced medical radiography, specifically in digital mammography and angiography. The research includes the acquisition of two-dimensional radiography of a mammography phantom using the scanning method, and its comparison with similar images simulated mathematically for different X rays sources. The paper also shows the experimental radiography of two biological samples taken from biopsies of mammas, where it is possible to identify the presence of possible pathological lesions. The results reached in this work point positively toward the effective possibility of satisfactorily introducing those advanced detectors in medical digital imaging applications. (Author)

  18. NCEI Marine Geology Data Archive

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Marine Geologic data compilations and reports in the NCEI archive are from academic and government sources around the world. Over ten terabytes of analyses,...

  19. The Operation and Architecture of the Keck Observatory Archive

    Science.gov (United States)

    Berriman, G. B.; Gelino, C. R.; Laity, A.; Kong, M.; Swain, M.; Holt, J.; Goodrich, R.; Mader, J.; Tran, H. D.

    2014-05-01

    The Infrared Processing and Analysis Center (IPAC) and the W. M. Keck Observatory (WMKO) are collaborating to build an archive for the twin 10-m Keck Telescopes, located near the summit of Mauna Kea. The Keck Observatory Archive (KOA) takes advantage of IPAC's long experience with managing and archiving large and complex data sets from active missions and serving them to the community; and of the Observatory's knowledge of the operation of its sophisticated instrumentation and the organization of the data products. By the end of 2013, KOA will contain data from all eight active observatory instruments, with an anticipated volume of 28 TB. The data include raw science and observations, quick look products, weather information, and, for some instruments, reduced and calibrated products. The goal of including data from all instruments is the cumulation of a rapid expansion of the archive's holdings, and already data from four new instruments have been added since October 2012. One more active instrument, the integral field spectrograph OSIRIS, is scheduled for ingestion in December 2013. After preparation for ingestion into the archive, the data are transmitted electronically from WMKO to IPAC for curation in the physical archive. This process includes validation of the science and content of the data and verification that data were not corrupted in transmission. The archived data include both newly-acquired observations and all previously acquired observations. The older data extends back to the date of instrument commissioning; for some instruments, such as HIRES, these data can extend as far back as 1994. KOA will continue to ingest all newly obtained observations, at an anticipated volume of 4 TB per year, and plans to ingest data from two decommissioned instruments. Access to these data is governed by a data use policy that guarantees Principal Investigators (PI) exclusive access to their data for at least 18 months, and allows for extensions as granted by

  20. Archiving in the networked world

    OpenAIRE

    Seadle, Michael

    2011-01-01

    Purpose – This paper aims to focus on how long-term digital archiving systems are tested and what benchmarks and other metrics are necessary for that testing to produce data that the community can use to make decisions. Design/methodology/approach – The article reviews recent literature about digital archiving systems involving public and semi-public tests. It then looks specifically at the rules and metrics needed for doing public or semi-public testing for three specific issues: triggeri...

  1. 两种样本生成方法对DRP-4DVar同化方法性能影响的比较及分析%A comparison of sensitivity of DRP-4DVar performance to perturbation samples obtained between the two different methods.

    Institute of Scientific and Technical Information of China (English)

    赵娟; 王斌

    2011-01-01

    The dimension-reduced projection-four dimensional variational data assimilation (DRP-4DVar) approach utilizes the ensemble of historical forecasts to estimate the background error covariance (BEC) and directly obtains the analysis on the ensemble space. As a result, the quality of ensemble members plays an important role in the performance of the DRP-4DVar approach. In this study, the BEC of the Weather Research and Forecast Model (WRF) 3-dimensional variational data assimilation (3DVar) system is employed to produce initial perturbation samples for the DRP-4DVar approach. The historical-forecast-based initial perturbation samples are flowdependent and able to describe the error-growing pattern in the atmospheric model and the balanced relationship between different model variables. However, the ensemble spread is not big enough because of the short time interval between adjacent historical samples and the limited numbers of ensemble. The perturbation method of control variables based on the structure characteristics of 3DVar BEC is able to produce initial perturbation samples that have reasonable background error correlations. Moreover, the estimated background error covariance also has good consistence and dynamic and physical balance between variables if the initial perturbation samples experience a development through 6 or 12 h model forward integration. From the aspect of computational expense, the historical forecast results can be directly obtained without any additional computational cost from operational numerical weather forecast centers, while the integration of samples from the 3DVar-based control variable method is time-consuming but this difficulty can be alleviated through parallel computing. With the analysis of the DRP-4DVar based on the new sample as the initial condition (IC), the 9 to 12 h forecasts are better, although the IC is a little worse than that from the DRP-4DVar using the historical-forecast-based sample. Moreover, the simulated

  2. The mycobiota associated with paper archives and their potential control

    Directory of Open Access Journals (Sweden)

    SAIFELDIN A. F. EL-NAGERABI

    2014-07-01

    Full Text Available El-Nagerabi SAF, Elshafie AE, Al-Hinai UA. 2014. The mycobiota associated with paper archives and their potential control. Nusantara Bioscience 6: 19-25. Historical collections kept in archives and libraries represent a cultural and artistic heritage of innumerable value. Recently in Oman, more than seventy thousand documents were collected from different countries and displayed as archives showed evident sign of mold contamination. The objectives of the present study were to screen these archives for mold invasion and a test for the effective control measure. For this, 102 samples were collected from documents of different sources and incubated on potato dextrose agar (PDA at ambient temperature (25○C±2. The isolated fungi were identified microscopically and confirmed with DNA extraction, PCR and DNA sequencing. Twenty-two fungal species belonging to 11 genera were recovered. The genus Penicillium (46.8% was the most prevalent, followed by Aspergillus (30.7%, Cladosporium (7%, Rhizopus (4%, and Chaetomium (3.5% whereas the remaining 6 genera represent only 8%. Eleven species were previously reported from similar substrates, whereas 11 species and one genus are new records for the mycoflora of archives. Sodium hypochlorite at 0.3-5.2% completely inhibited the fungal growth of the 10 tested fungal isolates with minimum inhibition concentration at 0.7%. Fumigation of books with 0.7-5.2% sodium hypochlorite completely inhibited all fungi without evident damage of the documents or ink discoloration. Therefore, sodium hypochlorite can be recommended as effective and eco-friendly disinfectant for archives comparable to other hazardous chemicals.

  3. Cambios histológicos en muestras de agrandamientos gingivales obtenidas a través de biopsias con electrobisturí y bisturí convencional Histological changes in samples of gingival overgrowth biopsies obtained through conventional scalpel and electro surgical scalpel

    Directory of Open Access Journals (Sweden)

    A. Herrera Herrera

    2012-06-01

    í eléctrico, que en oportunidades hicieron invalorables las muestra.Background. Literature Reports are scarce regarding histological changes observed on gingival tissues, but they are scarcer when gingival overgrowth from human tissues are considered. When histological slides are compared, the question arises if there are histological differences between samples obtained with electrosurgical versus conventional scalpel. Methods. A comparative study was performed in 21 patients, 90 samples. Cross control strategy was used to allocate the surgical site for each treatment, facilitating to evaluate both treatments in the same subject (electrosurgical versus conventional scalpel in a random manner for both arches. Data was entered in an Excel data base and analyzed with STATA 9.1 statistical software. Fischer´s exact test, p=0.05 significance level was used. Results. Collagen burning and coagulation were observed in 72.7% of the cases, being higher for connective tissue samples obtained with electrosurgical scalpel, 79.5% regarding burning, being higher with electrosurgical scalpel for epithelial tissue. Connective tissue showed inflammation in 95.6% of the cases for conventional scalpel, mediated by lymphocytes and plasmatic cells; neutrophils were seen in 17.8% of the sample and inflammation was seen in 100% of the samples for electrosurgical and conventional scalpels, which evidenced 97.8% lymphocytes and plasmatic cells and 37.8% neutrophils. Conclusions. Different histological phenomena were observed in gingival biopsies obtained with electrosurgical versus conventional scalpel; this facilitates understanding gingival overgrowth associated with orthodontic treatment. Some damage was observed in several biopsy samples which in some cases rendered the samples unusable for histological evaluation.

  4. Intelligent drug delivery systems obtained by radiation

    Energy Technology Data Exchange (ETDEWEB)

    Martellini, Flavia; Higa, Olga Z.; Takacs, Erzsebet; Safranj, Agneza; Yoshida, Masaru; Katakai, Ryoichi; Carenza, Mario

    1998-06-01

    Radiation-induced polymerization of acryloyl-L-proline methyl ester, an {alpha}-aminoacid-containing monomer, in the presence of a crosslinking agent and a hydrophilic monomer gave rise to polymer hydrogels whose water content at equilibrium was found to decrease as the swelling temperature increased. Some hydrogel samples were obtained with entrapped acetaminophen, an analgesic and antipyretic drug. It was ascertained that the release of the drug was controlled by both the hydrophilicity of the polymer matrices and the environmental temperature.

  5. Intelligent drug delivery systems obtained by radiation

    Science.gov (United States)

    Martellini, Flavia; Higa, Olga Z.; Takacs, Erzsebet; Safranj, Agneza; Yoshida, Masaru; Katakai, Ryoichi; Carenza, Mario

    1998-06-01

    Radiation-induced polymerization of acryloyl-L-proline methyl ester, an α-aminoacid-containing monomer, in the presence of a crosslinking agent and a hydrophilic monomer gave rise to polymer hydrogels whose water content at equilibrium was found to decrease as the swelling temperature increased. Some hydrogel samples were obtained with entrapped acetaminophen, an analgesic and antipyretic drug. It was ascertained that the release of the drug was controlled by both the hydrophilicity of the polymer matrices and the environmental temperature.

  6. Mining the XRT archive to probe the X-ray absorber structure in the AGN population

    CERN Document Server

    Ballo, L; Moretti, A; Della Ceca, R; Andreon, S; Caccianiga, A; Braito, V; Campana, S; Vignali, C

    2015-01-01

    One of the key ingredients of the Unified Model of Active Galactic Nuclei (AGN) is the presence of a torus-like optically thick medium composed by dust and gas around the putative supermassive black hole. However, the structure, size and composition of this circumnuclear medium are still matter of debate. To this end, the search for column density variations through X-ray monitoring on different timescales (months, weeks and few days) is fundamental to constrain size, kinematics and location of the X-ray absorber(s). Here we describe our project of mining the Swift-XRT archive to assemble a sample of AGN with extreme column density variability and determining the physical properties of the X-ray absorber(s). We also present the results obtained from a daily-weekly Swift-XRT follow-up monitoring recently performed on one of the most interesting new candidates for variability discovered so far, Mrk 915.

  7. Methods of obtaining thermodynamic data

    International Nuclear Information System (INIS)

    The types of thermodynamic data needed to predict behavior of high temperature systems such as an overheated nuclear reactor in which the fuel has been exposed to water and oxygen are discussed. Procedures for obtaining the needed data are reviewed. 14 refs

  8. Ferromanganese crusts as archives of deep water Cd isotope compositions

    Science.gov (United States)

    Horner, T. J.; SchöNbäChler, M.; RehkäMper, M.; Nielsen, S. G.; Williams, H.; Halliday, A. N.; Xue, Z.; Hein, J. R.

    2010-04-01

    The geochemistry of Cd in seawater has attracted significant attention owing to the nutrient-like properties of this element. Recent culturing studies have demonstrated that Cd is a biologically important trace metal that plays a role in the sequestration of inorganic carbon. This conclusion is supported by recent isotope data for Cd dissolved in seawater and incorporated in cultured phytoplankton. These results show that plankton features isotopically light Cd while Cd-depleted surface waters typically exhibit complimentary heavy Cd isotope compositions. Seawater samples from below 900 m depth display a uniform and intermediate isotope composition of ɛ114/110Cd = +3.3 ± 0.5. This study investigates whether ferromanganese (Fe-Mn) crusts are robust archives of deep water Cd isotope compositions. To this end, Cd isotope data were obtained for the recent growth surfaces of 15 Fe-Mn crusts from the Atlantic, Pacific, Indian, and Southern oceans and two USGS Fe-Mn reference nodules using double spike multiple collector inductively coupled plasma mass spectrometry. The Fe-Mn crusts yield a mean ɛ114/110Cd of +3.2 ± 0.4 (2 SE, n = 14). Data for all but one of the samples are identical, within the analytical uncertainty of ±1.1ɛ114/110Cd (2 SD), to the mean deep water Cd isotope value. This indicates that Fe-Mn crusts record seawater Cd isotope compositions without significant isotope fractionation. A single sample from the Southern Ocean exhibits a light Cd isotope composition of ɛ114/110Cd = 0.2 ± 1.1. The origin of this signature is unclear, but it may reflect variations in deep water Cd isotope compositions related to differences in surface water Cd utilization or long-term changes in seawater ɛ114/110Cd. The results suggest that time series analyses of Fe-Mn crusts may be utilized to study changes in marine Cd utilization.

  9. Models and methods for web archive crawling

    OpenAIRE

    Denev, Dimitar

    2012-01-01

    Web archives offer a rich and plentiful source of information to researchers, analysts, and legal experts. For this purpose, they gather Web sites as the sites change over time. In order to keep up to high standards of data quality, Web archives have to collect all versions of the Web sites. Due to limited resuources and technical constraints this is not possible. Therefore, Web archives consist of versions archived at various time points without guarantee for mutual consistency. This thes...

  10. Download - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open TG-GATEs Download... First of all, please read the license of this database. Data names and data descriptions are about the downloa...File Simple search and download 1 README README_e.html - 2 Compound list open_tggates_main.zip (6.4 KB) Simple search and download...te.zip (199 KB) Simple search and download 5 Cell sample open_tggates_cell.zip (54 KB) Simple search and download... 6 Cell viability open_tggates_cell_viability.zip (51 KB) Simple search and download 7 Individual list

  11. Truncated States Obtained by Iteration

    Institute of Scientific and Technical Information of China (English)

    W.B.Cardoso; N.G.de Almeida

    2008-01-01

    We introduce the concept of truncated states obtained via iterative processes(TSI)and study its statistical features,making an analogy with dynamical systems theory(DST).As a specific example,we have studied TSI for the doubring and the logistic functions,which are standard functions in studying chaos.TSI for both the doubling and logistic functions exhibit certain similar patterns when their statistical features are compared from the point of view of DST.

  12. 50 CFR 635.33 - Archival tags.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Archival tags. 635.33 Section 635.33..., DEPARTMENT OF COMMERCE ATLANTIC HIGHLY MIGRATORY SPECIES Management Measures § 635.33 Archival tags. (a) Implantation report. Any person affixing or implanting an archival tag into a regulated species must...

  13. Encoded Archival Description as a Halfway Technology

    Science.gov (United States)

    Dow, Elizabeth H.

    2009-01-01

    In the mid 1990s, Encoded Archival Description (EAD) appeared as a revolutionary technology for publishing archival finding aids on the Web. The author explores whether or not, given the advent of Web 2.0, the archival community should abandon EAD and look for something to replace it. (Contains 18 notes.)

  14. 36 CFR 1275.24 - Archival processing.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Archival processing. 1275.24... THE NIXON ADMINISTRATION Preservation and Protection § 1275.24 Archival processing. When authorized by the Archivist and until the commencement of archival processing in accordance with subpart D of...

  15. [Archival tags and geolocation methods for marine animals: A review].

    Science.gov (United States)

    Zhang, Tian-feng; Fan, Wei; Dai, Yang

    2015-11-01

    Archival tags, a group of data storable electronic tags, are widely used as strong tools for obtaining long term and large scale activity information of marine animals, specifically highly migratory oceanic fishes, and corresponding environmental data. Though retrieving tags is an indispensable step for obtaining data, which is a shortage of archival tags, a series of achievements have been made on marine animals by using archival tags since the 1990s. With the appearance of pop-up satellite tag, which solved the problem of data retrieving and was fully independent of the fishing, both breadth and depth of marine animals' studies are extended by the end of the 1990s. Geolocation based on light intensity is the key to estimate marine animals' movement and has achieved some progress in the past 20 years. However, the accuracy of geolocation for latitude is not high enough, and there is still much room for improvement. To date, most geolocation methods that use ambient daylight involve identifying the times when the sun is at a precisely known zenith angle (e.g., sunrise and sunset). The problem of estimating longitude has been proved easy to solve, but accurate latitude estimates remain elusive. This paper mainly introduced two tags, i. e., archival tags and pop-up tags, and three geolocation methods, i.e. , 1) the "fixed reference" method, 2) the "variable reference" method, and 3) the "reflection" method. We also presented a prospect analysis on archival tags and possible research direction of geolocation methods. We believed that miniaturization and multi-sensor integration are the trends for electronic tags while more environmental factors such as depth, SST (sea surface temperature) or magnetic field intensity, instead of single factor, as auxiliary parameters would be used for improving the geolocation accuracy in the future.

  16. The ``One Archive'' for JWST

    Science.gov (United States)

    Greene, G.; Kyprianou, M.; Levay, K.; Sienkewicz, M.; Donaldson, T.; Dower, T.; Swam, M.; Bushouse, H.; Greenfield, P.; Kidwell, R.; Wolfe, D.; Gardner, L.; Nieto-Santisteban, M.; Swade, D.; McLean, B.; Abney, F.; Alexov, A.; Binegar, S.; Aloisi, A.; Slowinski, S.; Gousoulin, J.

    2015-09-01

    The next generation for the Space Telescope Science Institute data management system is gearing up to provide a suite of archive system services supporting the operation of the James Webb Space Telescope. We are now completing the initial stage of integration and testing for the preliminary ground system builds of the JWST Science Operations Center which includes multiple components of the Data Management Subsystem (DMS). The vision for astronomical science and research with the JWST archive introduces both solutions to formal mission requirements and innovation derived from our existing mission systems along with the collective shared experience of our global user community. We are building upon the success of the Hubble Space Telescope archive systems, standards developed by the International Virtual Observatory Alliance, and collaborations with our archive data center partners. In proceeding forward, the “one archive” architectural model presented here is designed to balance the objectives for this new and exciting mission. The STScI JWST archive will deliver high quality calibrated science data products, support multi-mission data discovery and analysis, and provide an infrastructure which supports bridges to highly valued community tools and services.

  17. MESA: Mercator scheduler and archive system

    Science.gov (United States)

    Merges, Florian; Prins, Saskia; Pessemier, Wim; Raskin, Gert; Perez Padilla, Jesus; Van Winckel, Hans; Aerts, Conny

    2012-09-01

    We have developed an observing scheduling and archive system for the 1.2 meter Mercator Telescope. The goal was to optimize the specific niche of this modern small telescope in observational astrophysics: the building-up of long-term time series of photometric or high-resolution spectroscopic data with appropriate sampling for any given scientific program. This system allows PIs to easily submit their technical requirements and keep track of the progress of the observing programmes. The scheduling system provides the observer with an optimal schedule for the night which takes into account the current observing conditions as well as the priorities and requirements of the programmes in the queue. The observer can conveniently plan an observing night but also quickly adapt it to changing conditions. The archiving system automatically processes new files as they are created, including reduced data. It extracts the metadata and performs the normalization. A user can query, inspect and retrieve observing data. The progress of individual programmes, including timeline and reduced data plots can be seen at any time. Our MESA project is based on free and open source software (FOSS) using the Python programming language. The system is fully integrated with the Mercator Observing Control System1 (MOCS).

  18. Digitization of Archives of Astronomical Plates

    Science.gov (United States)

    Omizzolo, Alessandro; Cesare, Barbieri; Blanco, Carlo; Bucciarelli, Beatrice; di Paola, Andrea; Nesci, Roberto

    The photographic plate archives of telescopes around the world contain a veritable treasury of astronomical data. Unfortunately the emulsion is a volatile support and full exploitation of the scientific content is more and more difficult. A large-scale two-year project to digitize the archive of plates of the Italian Astronomical Observatories and of the Specola Vaticana has been started in 2002 with funds from the Ministry of the University and Research following a pilot program funded by the University of Padova in 2001. Identical systems composed by a high quality commercial scanner plus dedicated personal computers and acquisition software (developed initially at DLR Berlin) have been installed in all participating Institutes. Three main goals make up the total project: to provide high quality photometric sequences with the Campo Imperatore telescopes to be used on the scanned plates to perform astrometric measures taking advantage of the large span of time covered by the plates and to distribute the digitized information to all interested researchers via the international Web. This paper presents some of the activities carried out and results obtained so far

  19. Sample Set (SE): SE24 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available 32S-containing monoisotopic ions and their 34S-substituted counterparts for 67 S-containing monoisotopic...on bulbs. We performed comprehensive peak picking using the theoretical mass difference (1.99579 Da) between

  20. Sample Set (SE): SE14 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE14 Effect of agricultural films for tomato fruit metabolites Investigation of Sol...anum lycopersicum fruit metabolites. 3 growth conditions (covered with normal or UV cut agricultural film or no agricultural

  1. Sample Set (SE): SE27 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE27 Effect of agricultural films for cabbage leaf metabolites Investigation of Bra...ssica oleracea var. capitata YR Tenkuu leaf metabolites. 3 growth conditions (covered with normal or UV cut agricultural... film or no agricultural film), 3 replicates data are examined. Takeshi Ara 1, Naoki Yamamoto 1,

  2. Sample Set (SE): SE16 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE16 Effect of agricultural films for spinach leaf metabolites 2 Investigation of S...pinacia oleracea leaf metabolites. 3 growth conditions (covered with normal or UV cut agricultural film or no agricultural

  3. Sample (S): SE25_S02 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S02 Brassica oleracea var. capitata Satsuki Jouou Leaf Brassica oleracea var. ...capitata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  4. Sample (S): SE25_S06 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S06 Brassica oleracea var. capitata K7-705 Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  5. Sample (S): SE10_S02 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE10_S02 Brassica oleracea var. capitata Hatukoi Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Bra...ssica oleracea var. capitata plants are grown in the open field with low phosphorus condition (25kg/10a). ...

  6. Sample (S): SE25_S07 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S07 Brassica oleracea var. capitata T-767 Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Brass...ica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  7. Sample (S): SE25_S03 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S03 Brassica oleracea var. capitata Satsukiou Leaf Brassica oleracea var. capi...tata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  8. Sample (S): SE27_S02 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE27_S02 Brassica oleracea var. capitata YR Tenkuu Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Brassica oleracea var. capitata plants are grown at agricultural field under normal film. ...

  9. Sample (S): SE25_S05 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S05 Brassica oleracea var. capitata Hatukoi Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Bra...ssica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  10. Sample (S): SE25_S10 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S10 Brassica oleracea var. capitata Kanpachi Leaf Brassica oleracea var. capit...ata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  11. Sample (S): SE10_S03 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE10_S03 Brassica oleracea var. capitata Hatukoi Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Bra...ssica oleracea var. capitata plants are grown in the open field with no phosphorus condition (0kg/10a). ...

  12. Sample (S): SE25_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S01 Brassica oleracea var. capitata Kanroku Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Bra...ssica oleracea var. capitata plants are grown at agricultural field. ...

  13. Sample (S): SE10_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE10_S01 Brassica oleracea var. capitata Hatukoi Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Bra...ssica oleracea var. capitata plants are grown in the open field with high phosphorus condition (273.4kg/10a). ...

  14. Sample (S): SE27_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE27_S01 Brassica oleracea var. capitata YR Tenkuu Leaf Brassica oleracea var. capi...tata NCBI taxonomy:3716 Brassica oleracea var. capitata plants are grown at agricultural field. ...

  15. Sample (S): SE25_S09 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S09 Brassica oleracea var. capitata Teruyoshi Leaf Brassica oleracea var. capi...tata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  16. Sample (S): SE27_S03 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE27_S03 Brassica oleracea var. capitata YR Tenkuu Leaf Brassica oleracea var. capi...tata NCBI taxonomy:3716 Brassica oleracea var. capitata plants are grown at agricultural field under UV film. ...

  17. Sample (S): SE25_S08 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S08 Brassica oleracea var. capitata YR Tenkuu Leaf Brassica oleracea var. capi...tata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  18. Sample (S): SE26_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE26_S01 Brassica oleracea var. capitata YR Seisyun Leaf Brassica oleracea var. capitata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions with 0t compost treatment. ...

  19. Sample (S): SE25_S04 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE25_S04 Brassica oleracea var. capitata Kinkei201gou Leaf Brassica oleracea var. c...apitata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions. ...

  20. Sample (S): SE26_S02 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE26_S02 Brassica oleracea var. capitata YR Seisyun Leaf Brassica oleracea var. cap...itata NCBI taxonomy:3716 Brassica oleracea var. capitata are grown at agricultural field in natural conditions with 6t compost treatment. ...

  1. Sample Set (SE): SE52 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available tabolite identifier for identification and putative annotation. A simple metaboli...compound database, were employed for the structural elucidation. The outputs were retrieved using the CAS me

  2. Sample Set (SE): SE18 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE18 Global identification of phospholipid molecular species in mouse liver and bra...in by Orbitrap MS and automated search engine Lipid Search A comprehensive and precise identification of pho

  3. Sample (S): SE52_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available s were soaked on MS agar plates and then incubated at 22°C under 16 h day and 8 h night conditions. At 18 days after germination, the aerial parts of the seedlings were harvested. ...

  4. Sample (S): SE58_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available red at the meristem of each tomato plant (light source: Ceramic metal halide lamps). Subirrigation was app...lied twice a day with the nutrient solution, and plant material was harvested three weekdays after flowering in December 2009. ...

  5. Sample Set (SE): SE10 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE10 Effect of phosphorus nutrition for cabbage metabolites Investigation of Brassi...ca oleracea var. capitata Hatukoi leaf metabolites. 3 growth conditions (high, low and no phosphorus nutrition

  6. Sample Set (SE): SE57 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available ster analysis based on the metabolite accumulation patterns clearly showed differences among the plant famil...etabolite accumulation patterns in plants We optimized the MRM conditions for specifi c compounds by perform...SE57 Widely targeted metabolomics based on large-scale MS/MS data for elucidating m

  7. Sample (S): SE3_S02 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE3_S02 Solanum lycopersicum House Momotaro fruit Solanum lycopersicum NCBI taxonom...y:4081 Solanum lycopersicum House Momotaro (ref: http://www.e-taneya.com/item/914.html) are grown at agricultural field under normal film in natural conditions. ...

  8. Sample (S): SE11_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available ng was done at May 31 and fix planting was done at June 28 in the pots with synthet...1 Solanum lycopersicum Furikoma were grown in a greenhouse located in Chiba, Japan. Seeds were sown at May 11, 2011. Temporary planti

  9. Sample (S): SE45_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available o quench enzymatic activity. The all raw data are also available in MetaboLights (Salek et al., 2013) (accession no. MTBLS47), and The MetabolomeExpress (Carroll et al., 2010). ...

  10. Sample Set (SE): SE20 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE20 Arabidopsis thaliana leaf metabolite analysis for a software test Investigatio...es that were investigated to optimize the parameters of PowerGet software for better metabolite annotation i

  11. Sample Set (SE): SE41 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available 1, Daisuke Shibata 1, 2, 1:Graduate School of Agriculture, Kyoto University, Japan, 2:Kazusa DNA Research Institute, Japan, 3:KAGOME CO., LTD., Japan Direct Submittion version 1 ...

  12. Sample Set (SE): SE17 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Sakurai 1, Hideyuki Suzuki 1, Tatsuya Suzuki 2, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Chiba Prefectural Agriculture Research Center Direct Submittion version 1 ...

  13. Sample Set (SE): SE37 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available d data and blank data (S17). The isotopic peaks were searched and listed using ShiftedIonsFinder... (S18). Moreover, the flavonoid-like peaks were also searched using ShiftedIonsFinder (S19). ...hiba 285-0801, Japan ShiftedIonsFinder: A standalone Java tool for finding peaks with specified mass differe

  14. Sample Set (SE): SE54 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available ic profiling of large-scale bioresources We established a novel methodology, termed widely targeted metabolo...ucted a targeted metabolite analysis of large-scale Arabidopsis bioresources, nam

  15. Sample Preparation (SS): SE45_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available er mill MM310 at a frequency of 30 Hz for 3 min at 4°C. Each isotope compound was adjusted to a final concen...]) containing 10 stable isotope reference compounds: • [2H4]-succinic acid • [13C5,15N]-glutamic acid • [2H7...tion of 5 mg flesh weight (FW) of tissues per ml extraction medium (methanol / chloroform/water [3:1:1 v/v/v

  16. Sample Set (SE): SE51 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available o be narrowed down to candidate structures. Therefore, a web-based database of MS/MS data pertaining to phyt...ined from authentic standards. As a main web application of ReSpect, a fragment search was established based...re successfully narrowed down to putative structures in the website. Yuji Sawada,

  17. Sample Set (SE): SE34 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available examined. Yukiko Nakamura, Takeshi Ara, Takashi Matsuura, Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata, Kazusa DNA Research Institute Direct Submittion version 1 ...

  18. Sample Set (SE): SE28 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available mined. Yukiko Nakamura, Takeshi Ara, Takashi Matsuura, Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata, Kazusa DNA Research Institute Direct Submittion version 1 ...

  19. Sample Set (SE): SE40 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available fruits metabolites. 28 cultivers and 3 replicates data are examined. Takeshi Ara 1, Shingo Takahashi 1,3, Naoko Waki 1,3, Nozomu Sak...urai 2, Haruya Takahashi 1, Shinsuke Mohri 1, Kunimi Suda 2, K. Ozawa 2, Takisawa R

  20. Sample Set (SE): SE46 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available lar processes. The modes of regulation at the metabolome level can be revealed by metabolic network...s. We investigated the metabolic network between wild-type and 2 mutant (methionine-over a...o1 were much lower than those of the wild-type and tt4 plants, indicating the loss of overall network stabil...esting an adaptive reconfiguration of the network. Gene-expression correlations p...resumably responsible for these metabolic networks were determined using the metabolite correlations as clue

  1. Sample Preparation (SS): SE60_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available s methanol containing an internal standard (0.5 mgL-1 lidocaine, Tokyo Kasei, Tokyo, Japan, http://www.tcich...emicals.com/), using a mixer mill (MM 300, Retsch, Haan, Germany, http://www.rets

  2. Sample Set (SE): SE60 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available cies produces more diverse phytochemicals than previously expected, and plants still contain many useful compounds for human applicat...ions. Fumio Matsuda, Ryo Nakabayashi, Zhigang Yang, Yozo

  3. Sample (S): SE59_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE59_S01 Arabidopsis Arabidopsis thaliana NCBI taxonomy:3702 Arabidopsis (Col-0) pl...ants were used in this study. All plates were placed in a growth chamber at 22 °C with a relative humidity o

  4. Sample Preparation (SS): SE50_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available sing MeV 4.8. The data discussed here were also deposited in NCBI's Gene Expression Omnibus (Edgar et al., 2...c and abiotic stress-related genes. The log2-transformed value was used for HCA u

  5. Sample Set (SE): SE45 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available aphy-mass spectrometry. To make the data set available as an efficient public functional genomics tool for h... for genes of interest and for improving gene annotation. MeKO is publicly available at http://prime.psc.rik

  6. Sample (S): SE22_S02 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the contro...awn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical

  7. Sample (S): SE22_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the contro...awn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical

  8. Sample (S): SE22_S06 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the contro...awn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical

  9. Sample (S): SE22_S08 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the contro...awn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical

  10. Sample (S): SE22_S05 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the contro...awn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical

  11. Sample (S): SE22_S03 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the contro...awn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical

  12. Sample (S): SE22_S04 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the contro...awn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical

  13. Sample (S): SE22_S07 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the contro...awn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical

  14. Sample (S): SE40_S26 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE40_S26 Solanum lycopersicum Sicilian Rouge fruit Solanum lycopersicum NCBI taxono...my:4081 Solanum lycopersicum Sicilian Rouge were purchased at a local market of Kyoto city, (Kyoto, Japan) (November, 2015). Kyoto-san. ...

  15. Sample Set (SE): SE13 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available ia oleracea leaf metabolites. 4 cultivers (Nihon, Kanaji, Kurohaminstarland and Wasesaradaakari) and 3 repli...1, Hideyuki Suzuki 1, Tatsuya Suzuki 2, Daisuke Shibata 1, 1: Kazusa DNA Research Inst

  16. Sample Preparation (SS): SE54_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available tonitrile in H2O with 25 μM hydroxyphenyl–glucosinolate (GSL) and 50 μM norleucine ...s of each independent mutant and accession were homogenized using a mixer mill MM 200 (Retsch) in 80 μL of extraction buffer [40% ace

  17. Sample (S): SE19_S2 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE19_S2 Mouse white adipose tissue (WAT) Mus musculus NCBI taxonomy:10090 Mice (C57...BL/6) were purchased from SLC, Shizuoka, Japan. White adipose tissue was extracted and used. ...

  18. Sample Set (SE): SE19 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE19 Grobal triacylglycerol analysis in mouse liver and white adipose tissue (WAT) ...rol (TAG) molecular species from complex lipid mixtures of mouse liver and white adipose tissue (WAT) using

  19. Sample Set (SE): SE30 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE30 Comparison of seed metabolites among soybean varieties Investigation of Glycine max. green seed metabol...ites. 3 varieties (Kamogawashichiri, Koitozairai, Tanbakuro) data are examined. Tak

  20. Sample Set (SE): SE31 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE31 Comparison of seed metabolites among eggplant fruit tissues Investigation of S...olanum melongena Senryounigou metabolites. 3 tissues (flesh, fruit, peel) data are examined. Takeshi Ara 1,

  1. Sample Set (SE): SE3 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE3 Comparison of fruit metabolites among tomato varieties 1 Investigation of Solanum lycopersicum fruit met...abolites. 3 caltivars and an original species, 3 replicates data are examined. Naok

  2. Sample Set (SE): SE26 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE26 Effect of compost for cabbage metabolites Investigation of Brassica oleracea v...ar. capitata YR Seisyun leaf metabolites. 2 growth conditions (with or without 6t compost treatment) and 3 r

  3. Sample (S): SE36_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available plants are grown on pots filled by soil under natural conditions. Plants are grown at room temperature with natural light condition in winter. Leaves are harvested at spring of 2008. ...

  4. Sample (S): SE53_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available tted in 1/2000 a Wagner pot containing compost soil (Kureha, Tokyo, Japan) for the soil experiment. Seeds we... pilot and HC experiments) and late summer (the soil experiment) in 2006, 2008, and 2009.

  5. Sample Set (SE): SE56 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE56 MS/MS spectral tag-based annotation of non-targeted profile of plant secondary...sis, resulting in clarification of the functions of reported genes involved in glycosylation of flavonoids. Thus, non-target

  6. Sample Set (SE): SE59 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available metabolites of drought stress, e.g., proline, raffinose, and galactinol. These findings indicate that flavon...n of 5 flavonols and 5 anthocyanins was revealed together with changes in marker

  7. Sample Set (SE): SE48 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available e public mass spectral data depositories, including the plant-specific MS/MS-baseddatabase, ReSpect. Zhi...a,Jun Kikuchi,Kazuki Saito Zhigang Y et al. (2014) Metabolomics 10: 543-555 ...

  8. Sample (S): SE40_S27 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE40_S27 Solanum lycopersicum Carol7 (Karin) fruit Solanum lycopersicum NCBI taxono...my:4081 Solanum lycopersicum Carol7 (Karin) were purchased at a local market of Kyoto city, (Kyoto, Japan) (November, 2015). Aichi-san. ...

  9. Sample Preparation (SS): SE58_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available anoic acid, [2H4]-1,4-butanediamine, [2H6]-2-hydoxybenzoic acid, and 13C6]-glucose. These internal standards... were used to normalize the data using cross-contribution compensating mutipl standard normalization (CCMN).

  10. Sample (S): SE56_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available 6.3 (Boyes et al., 2001) and stored at 80℃ until use. For metabolic phenotyping of Ds transposon insertion l...ines (Kuromori et al., 2004, 2006), 60 lines of homozygous seeds were grown on th

  11. Sample (S): SE54_S01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available er natural light at 22℃ with application of 2,000-fold-diluted Hyponex until flowering finished. Then seeds ... were grown for 3 weeks under a 16-h light/8-h dark cycle at 22℃, transferred to soil and grown under the same conditions with applic...ation of 2,000-fold diluted Hyponex (Hyponex Japan Co Ltd., Osaka, Japan) until see

  12. Sample Set (SE): SE12 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE12 Effect of agricultural films for spinach leaf metabolites Investigation of Spi...nacia oleracea leaf metabolites. 2 cultivars (Nihon and Wasesaradaakari), 2 growth conditions (normal film and UV film

  13. Sample Set (SE): SE55 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE55 AtMetExpress development: a phytochemical atlas of arabidopsis development We analyzed phytochemical...idopsis produces various phytochemicals in a highly tissue-specific manner, which often accompanies the expr

  14. Sample Set (SE): SE53 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE53 Covering chemical diversity of genetically-modified tomatoes using metabolomic...rect acquisition of an interpretable data set of estimable chemical diversity. As an example, we report an a...etected compounds that represent 86% of the estimated chemical diversity of the metabolites listed in the Ly

  15. License - Society Catalog | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Socie...ke 2.1 Japan . If you use data from this database, please be sure attribute this database as follows: Societ...y Catalog © The Database Center for Life Science licensed under CC Attribution-Sh...a license for use of this database or any part thereof not licensed under the license. National Bioscience Database Center Japan Scie...se Update History of This Database Site Policy | Contact Us License - Society Catalog | LSDB Archive ...

  16. Download - RGP physicalmap | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP physicalma...e File Simple search and download 1 README README_e.html - 2 YAC contig information rgp_physicalmap_yac_cont...igs.zip (1 KB) Simple search and download 3 YAC clone information rgp_physicalmap_yac_clones.zip (18.7 KB) S...imple search and download 4 Insert size of YAC clones rgp_physicalmap_insert_size...ase Description Download License Update History of This Database Site Policy | Contact Us Download - RGP physicalmap | LSDB Archive ...

  17. Download - Society Catalog | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Society Catalog Download...e File Simple search and download 1 README README_e.html - 2 Society Catalog Information AcademyCatalog_en.z...ip (41.9 KB) - Downlaod via FTP Joomla SEF URLs by Artio About This Database Database Description Download L...icense Update History of This Database Site Policy | Contact Us Download - Society Catalog | LSDB Archive ... ...tions are about the downloadable data in this page. They might not correspond to the contents of the origina

  18. Download - RGP caps | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP caps Download... First of all, please read the license of this database. Data names and data descriptions are about the download...ase. Click the links on Data Name for descriptions of the data. # Data name File Simple search and downloa..._main.zip (18.5 KB) Simple search and download 3 Images of gel electrophoresis rgp_caps_electrophoresis_imag...atabase Description Download License Update History of This Database Site Policy | Contact Us Download - RGP caps | LSDB Archive ...

  19. Download - DGBY | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...DGBY Download First of all, please read the license of this database. Data names and data descriptions are a...bout the downloadable data in this page. They might not correspond to the contents of the original database....ir-drying stress GEO Accession No: GSE6454 - 6 Phenome data - High-sugar stress CSV: dbgy_high_sugar_stress....od via FTP Joomla SEF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us Download - DGBY | LSDB Archive ...

  20. Download - RMG | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...RMG Download First of all, please read the license of this database. Data names and data descriptions are ab...out the downloadable data in this page. They might not correspond to the contents of the original database. ....zip (1 KB) Simple search and download Downlaod via FTP Joomla SEF URLs by Artio About Thi...s Database Database Description Download License Update History of This Database Site Policy | Contact Us Download - RMG | LSDB Archive ...

  1. Download - DMPD | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...DMPD Download First of all, please read the license of this database. Data names and data descriptions are a...bout the downloadable data in this page. They might not correspond to the contents of the original database....MB) - Downlaod via FTP Joomla SEF URLs by Artio About This Database Database Description Download License Update History of Thi...s Database Site Policy | Contact Us Download - DMPD | LSDB Archive ...

  2. Filtration and Leach Testing for REDOX Sludge and S-Saltcake Actual Waste Sample Composites

    Energy Technology Data Exchange (ETDEWEB)

    Shimskey, Rick W.; Billing, Justin M.; Buck, Edgar C.; Daniel, Richard C.; Draper, Kathryn E.; Edwards, Matthew K.; Geeting, John GH; Hallen, Richard T.; Jenson, Evan D.; Kozelisky, Anne E.; MacFarlan, Paul J.; Peterson, Reid A.; Snow, Lanee A.; Swoboda, Robert G.

    2009-02-20

    A testing program evaluating actual tank waste was developed in response to Task 4 from the M-12 External Flowsheet Review Team (EFRT) issue response plan.( ) The test program was subdivided into logical increments. The bulk water-insoluble solid wastes that are anticipated to be delivered to the Waste Treatment and Immobilization Plant (WTP) were identified according to type such that the actual waste testing could be targeted to the relevant categories. Under test plan TP-RPP-WTP-467, eight broad waste groupings were defined. Samples available from the 222S archive were identified and obtained for testing. Under this test plan, a waste-testing program was implemented that included: • Homogenizing the archive samples by group as defined in the test plan • Characterizing the homogenized sample groups • Performing parametric leaching testing on each group for compounds of interest • Performing bench-top filtration/leaching tests in the hot cell for each group to simulate filtration and leaching activities if they occurred in the UFP2 vessel of the WTP Pretreatment Facility. This report focuses on filtration/leaching tests performed on two of the eight waste composite samples and follow-on parametric tests to support aluminum leaching results from those tests.

  3. Electric-Magnetic-Waves Resident Archive for Polar (EMWRAP)

    Science.gov (United States)

    Hart, R.; Chi, P. J.; Faden, J.; Granroth, L. J.; Menietti, J. D.; Mozer, F. S.; Russell, C. T.; Vernetti, J.

    2010-12-01

    EMWRAP is a NASA-sponsored resident archive (RA) for the Electric Field Instrument (EFI), Magnetic Field Experiment (MFE), and Plasma Wave Instrument (PWI) data collected by the Polar satellite. The main objective of EMWRAP is to maintain the data availability to the scientific community after Polar ended its operation in April 2008. With more than eleven years of observations, Polar sampled all the regions of the magnetosphere inside a distance of 9 Re, and its dataset continues to be valuable for magnetospheric research through the use of these data either alone or with other joint observations. In this presentation we demonstrate the existing capability of disseminating Polar EFI, MFE, and PWI data, and we also describe the ongoing efforts to construct the RA, such as: (1) Establishing a central web server and two backup servers, each equipped with the entire data archive to safeguard against data loss; (2) Generating documentation for the data sets following widely accepted standards, and archiving the expertise in data interpretation for the use by future researchers; (3) Providing the metadata to fully integrate with the Virtual Magnetospheric Observatory (VMO) and other components of NASA's Heliophysics Data Environment (HPDE). When these essential functions are established, a copy of the data products and documentation will be submitted to NSSDC for permanent archives.

  4. Back to the Future: Long-Term Seismic Archives Revisited

    Science.gov (United States)

    Waldhauser, F.; Schaff, D. P.

    2007-12-01

    Archives of digital seismic data recorded by seismometer networks around the world have grown tremendously over the last several decades helped by the deployment of seismic stations and their continued operation within the framework of monitoring seismic activity. These archives typically consist of waveforms of seismic events and associated parametric data such as phase arrival time picks and the location of hypocenters. Catalogs of earthquake locations are fundamental data in seismology, and even in the Earth sciences in general. Yet, these locations have notoriously low spatial resolution because of errors in both the picks and the models commonly used to locate events one at a time. This limits their potential to address fundamental questions concerning the physics of earthquakes, the structure and composition of the Earth's interior, and the seismic hazards associated with active faults. We report on the comprehensive use of modern waveform cross-correlation based methodologies for high- resolution earthquake location - as applied to regional and global long-term seismic databases. By simultaneous re-analysis of two decades of the digital seismic archive of Northern California, reducing pick errors via cross-correlation and model errors via double-differencing, we achieve up to three orders of magnitude resolution improvement over existing hypocenter locations. The relocated events image networks of discrete faults at seismogenic depths across various tectonic settings that until now have been hidden in location uncertainties. Similar location improvements are obtained for earthquakes recorded at global networks by re- processing 40 years of parametric data from the ISC and corresponding waveforms archived at IRIS. Since our methods are scaleable and run on inexpensive Beowulf clusters, periodic re-analysis of entire archives may thus become a routine procedure to continuously improve resolution in existing catalogs. We demonstrate the role of seismic archives

  5. Disembodied archives: The disconnectedness of records and archives management practices within the Pietermaritzburg Cluster of Theological Libraries, KwaZulu-Natal, South Africa

    Directory of Open Access Journals (Sweden)

    Francis Garaba

    2015-03-01

    Full Text Available This article discusses the findings of a study that was conducted between February 2013 and November 2013 to determine the inter-connectedness between records and archives management practices for religious archives within the Pietermaritzburg Cluster of Theological libraries (PCTL. It is important to note that much of our national heritage is recorded in the archives of our religious institutions, hence the need to ensure that a continuum of care is provided for this Christian heritage from the point of creation to the retirement of those records with enduring value. Data for this study were obtained primarily through a selfadministered questionnaire, interviews and direct observation. The study revealed that there is much disharmony with regards to records and archives management-practices, which explains why documentary records in custody were incomplete. This incompleteness threatens the corporate memory of these institutions. An important recommendation proposed is the urgent need for archivists within these establishments to adopt a proactive stance in order to ensure that records should not only be properly managed for business continuity but that a healthy and complete record should be transferred to the repository for the benefit of posterity. Furthermore, there is a need to establish a central depository to coordinate records and archives management-activities, thus ensuring best practices in records and archives management.

  6. Physical Review Online Archives (PROLA)

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, T.; Davies, J.; Kilman, D.; Laroche, F. [and others

    1997-05-01

    In cooperation with the American Physical Society, the Computer Research and Applications Group (CIC-3 -- see Section 13 for an acronym glossary) at Los Alamos National Laboratory has developed and deployed a journal archive system called, The Physical Review OnLine Archive (PROLA). It is intended to be a complete, full service on-line archive of the existing issues of the journal Physical Review from its inception to the advent of a full-service electronic version. The fundamental goals of PROLA are to provide screen-viewable and printable images of every article, full-text and fielded search capability, good browsing features, direct article retrieval tools, and hyperlinking to all references, errata, and comments. The research focus is on transitioning large volumes of paper journals to a modern electronic environment.

  7. Central versus institutional self-archiving

    OpenAIRE

    Harnad, Stevan

    2006-01-01

    NIH's, PLoS's, the Wellcome Trust's and now the UK MRC's unreflective support for PubMed Central (PMC), a Central Repository (CR), as the locus for direct self-archiving by authors is very unfortunate for Institutional Repositories (IRs), for self-archiving, and for Open Access (OA) progress in general. Alma Swan has published key papers on both OA self-archiving policy and institutional versus central self-archiving (IRs vs. CRs) analysing the reasons. (a) Institutional self-archiving and ce...

  8. Boat sampling

    International Nuclear Information System (INIS)

    This presentation describes essential boat sampling activities: on site boat sampling process optimization and qualification; boat sampling of base material (beltline region); boat sampling of weld material (weld No. 4); problems accompanied with weld crown varieties, RPV shell inner radius tolerance, local corrosion pitting and water clarity. The equipment used for boat sampling is described too. 7 pictures

  9. Archival analyses of eyewitness identification test outcomes: what can they tell us about eyewitness memory?

    Science.gov (United States)

    Horry, Ruth; Halford, Paul; Brewer, Neil; Milne, Rebecca; Bull, Ray

    2014-02-01

    Several archival studies of eyewitness identification have been conducted, but the results have been inconsistent and contradictory. We identify some avoidable pitfalls that have been present in previous analyses and present new data that address these pitfalls. We explored associations among various estimator variables and lineup outcomes for 833 "real life" lineups, including 588 lineups in which corroborating evidence of the suspect's guilt existed. Suspect identifications were associated with exposure duration, viewing distance, and the age of the witness. Nonidentifications were associated with the number of perpetrators. We also consider some of the inherent, unavoidable limitations with archival studies and consider what such studies can really tell researchers. We conclude that differences in sampling prohibit sensible comparisons between the results of laboratory and archival studies, and that the informational value of archival studies is actually rather limited.

  10. Theoretical model of digital archives

    Directory of Open Access Journals (Sweden)

    Alenka Kavčič-Čolić

    2004-01-01

    Full Text Available There are certain conditions that national organizations should fulfill to became trusted digital repositories. Among other things, their deposit system should be in compliance with the reference model for an Open Archival Information System (OAIS. In this paper the model OAIS is presented as well as some important international experiences regarding its implementation. The joint project of the National and University Library and The Jozef Stefan Institute, which is aimed at developing such a digital archive based on OAIS, is also mentioned.

  11. Archiving in the networked world

    OpenAIRE

    Seadle, Michael

    2011-01-01

    Purpose – The purpose of this paper is to investigate how extensively LOCKSS/CLOCKSS, Portico, and e-Depot provide long-term digital archiving for the journals listed in the Directory of Open Access Journals (DOAJ). Design/methodology/approach – The paper uses publicly available online data, which are processed in a set of PERL programs to measure the number of DOAJ articles in the three archiving systems. Findings – The findings show that only 8 per cent of the DOAJ titles are in LOCKS...

  12. Demonstration of Entrained Solids and Sr/TRU Removal Processes with Archived AN-107 Waste

    Energy Technology Data Exchange (ETDEWEB)

    RT Hallen; KP Brooks; LK Jagoda

    2000-08-02

    Archived AN-107 waste was used to evaluate entrained solids removal, Sr/TRU decontamination of supernatant, and Sr/TRU solids removal. Even though most of the entrained solids had been previously removed from the archived sample, the residual entrained solids rapidly fouled the filter element resulting in very poor filter performance. An attempt to run at higher pressure resulted in more fouling, and reduced filter performance. Filtration efforts to remove entrained solids were abandoned and the waste was treated for Sr/TRU removal with the entrained solids present. The new processing scheme for Sr/TRU removal involving precipitation by added strontium and permanganate worked well. The decontamination factors for Sr and TRU components were significantly greater than the ILAW DF requirements for higher reagent concentrations of 1M hydroxide, 0.075M Sr, and 0.05M permanganate and lower reagent concentrations of 0.8M hydroxide, 0.05M Sr, and 0.03M permanganate. These results support the use of lower concentration of reagent additions in future tests. Optimization studies should be conducted to examine the reduction in added hydroxide from 1M to 0.5 M, reduction of Sr from 0.075M to 0.05M, and reduction in permanganate from 0.05M to 0.03M and the impact this reduction has on filtration performance with new samples from Tank AN-107. The combined entrained solids and Sr/TRU precipitate were successfully filtered in the single element, crossflow filtration unit. The filtrate flux was high, >0.1 gpm/ft{sup 2}, at the initial test conditions of 53 psi and 11.2ft/s for the treated archived AN-107 sample. The filter flux rate dropped significantly with time as testing progressed and appears to be a result of shearing the agglomerated solids and fouling of the filter element by the resulting fine particles. The relatively low clean water flux rates obtained at the end of the test also indicate filter fouling. Chemical cleaning was required to restore clean water flux rates to

  13. Demonstration of Entrained Solids and Sr/TRU Removal Processes with Archived AN-107 Waste

    International Nuclear Information System (INIS)

    Archived AN-107 waste was used to evaluate entrained solids removal, Sr/TRU decontamination of supernatant, and Sr/TRU solids removal. Even though most of the entrained solids had been previously removed from the archived sample, the residual entrained solids rapidly fouled the filter element resulting in very poor filter performance. An attempt to run at higher pressure resulted in more fouling, and reduced filter performance. Filtration efforts to remove entrained solids were abandoned and the waste was treated for Sr/TRU removal with the entrained solids present. The new processing scheme for Sr/TRU removal involving precipitation by added strontium and permanganate worked well. The decontamination factors for Sr and TRU components were significantly greater than the ILAW DF requirements for higher reagent concentrations of 1M hydroxide, 0.075M Sr, and 0.05M permanganate and lower reagent concentrations of 0.8M hydroxide, 0.05M Sr, and 0.03M permanganate. These results support the use of lower concentration of reagent additions in future tests. Optimization studies should be conducted to examine the reduction in added hydroxide from 1M to 0.5 M, reduction of Sr from 0.075M to 0.05M, and reduction in permanganate from 0.05M to 0.03M and the impact this reduction has on filtration performance with new samples from Tank AN-107. The combined entrained solids and Sr/TRU precipitate were successfully filtered in the single element, crossflow filtration unit. The filtrate flux was high, >0.1 gpm/ft2, at the initial test conditions of 53 psi and 11.2ft/s for the treated archived AN-107 sample. The filter flux rate dropped significantly with time as testing progressed and appears to be a result of shearing the agglomerated solids and fouling of the filter element by the resulting fine particles. The relatively low clean water flux rates obtained at the end of the test also indicate filter fouling. Chemical cleaning was required to restore clean water flux rates to pre

  14. Sampling Procedures for Coordinating Stratified Samples : Methods Based on Microstrata

    OpenAIRE

    Nedyalkova, Desislava; Tillé, Yves; Pea, Johan

    2016-01-01

    The aim of sampling coordination is to maximize or minimize the overlap between several samples drawn successively in a population that changes over time. Therefore, the selection of a new sample will depend on the samples previously drawn. In order to obtain a larger (or smaller) overlap of the samples than the one obtained by independent selection of samples, a dependence between the samplesmust be introduced. This dependence will emphasize (or limit) the number of common units in the selec...

  15. Images - RPSD | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ures of the proteins of plant seven kinds, such as rice. Data file File name: rpsd_images.zip File URL: ftp:...//ftp.biosciencedbc.jp/archive/rpsd/LATEST/rpsd_images.zip File size: 18.3 MB Sim

  16. The CADC/ST-ECF Archives of HST Data: Less is More

    Science.gov (United States)

    Crabtree, Dennis R.; Durand, Daniel; Gaudet, Severin; Hill, Norman; Pirenne, Beno\\^It

    The CADC and ST-ECF maintain copies of the HST archive in coordination with the STScI. We are continually exploring ways in which to make the archive a more powerful scientific tool for researchers (previous examples include: the science table, preview images, and a prototype Web interface). We also seek out innovative, cost-effective methods for accessing the HST archive. We have developed a new approach to the HST archive which not only makes it more useful scientifically, but also uses new technologies to provide more cost-effective service to our users. The CADC and ST-ECF now supports on-line access to the HST archive through a CD-ROM jukebox. In addition, we also support on-the-fly reprocessing of HST data when it is requested from the archive. This ensures that archival researchers can obtain data calibrated with the latest software and reference files. Performing on-the-fly recalibration allows to keep only the raw data on-line and we further subset this by only selecting data for the science targets. Finally, the raw data is compressed using gzip before being written to CD-ROM. Taken together the complete set of public HST data, i.e., data obtained until November 1994, fits on approximately 31 CDs! This paper will describe overall architecture of the archive including the Web access, maintenance of data on CD-ROM, maintenance of the reference data and details of the recalibration pipeline. Possible extensions for the future will also be discussed.

  17. High quality genomic copy number data from archival formalin-fixed paraffin-embedded leiomyosarcoma: optimisation of universal linkage system labelling.

    Science.gov (United States)

    Salawu, Abdulazeez; Ul-Hassan, Aliya; Hammond, David; Fernando, Malee; Reed, Malcolm; Sisley, Karen

    2012-01-01

    Most soft tissue sarcomas are characterized by genetic instability and frequent genomic copy number aberrations that are not subtype-specific. Oligonucleotide microarray-based Comparative Genomic Hybridisation (array CGH) is an important technique used to map genome-wide copy number aberrations, but the traditional requirement for high-quality DNA typically obtained from fresh tissue has limited its use in sarcomas. Although large archives of Formalin-fixed Paraffin-embedded (FFPE) tumour samples are available for research, the degradative effects of formalin on DNA from these tissues has made labelling and analysis by array CGH technically challenging. The Universal Linkage System (ULS) may be used for a one-step chemical labelling of such degraded DNA. We have optimised the ULS labelling protocol to perform aCGH on archived FFPE leiomyosarcoma tissues using the 180k Agilent platform. Preservation age of samples ranged from a few months to seventeen years and the DNA showed a wide range of degradation (when visualised on agarose gels). Consistently high DNA labelling efficiency and low microarray probe-to-probe variation (as measured by the derivative log ratio spread) was seen. Comparison of paired fresh and FFPE samples from identical tumours showed good correlation of CNAs detected. Furthermore, the ability to macro-dissect FFPE samples permitted the detection of CNAs that were masked in fresh tissue. Aberrations were visually confirmed using Fluorescence in situ Hybridisation. These results suggest that archival FFPE tissue, with its relative abundance and attendant clinical data may be used for effective mapping for genomic copy number aberrations in such rare tumours as leiomyosarcoma and potentially unravel clues to tumour origins, progression and ultimately, targeted treatment.

  18. High quality genomic copy number data from archival formalin-fixed paraffin-embedded leiomyosarcoma: optimisation of universal linkage system labelling.

    Directory of Open Access Journals (Sweden)

    Abdulazeez Salawu

    Full Text Available Most soft tissue sarcomas are characterized by genetic instability and frequent genomic copy number aberrations that are not subtype-specific. Oligonucleotide microarray-based Comparative Genomic Hybridisation (array CGH is an important technique used to map genome-wide copy number aberrations, but the traditional requirement for high-quality DNA typically obtained from fresh tissue has limited its use in sarcomas. Although large archives of Formalin-fixed Paraffin-embedded (FFPE tumour samples are available for research, the degradative effects of formalin on DNA from these tissues has made labelling and analysis by array CGH technically challenging. The Universal Linkage System (ULS may be used for a one-step chemical labelling of such degraded DNA. We have optimised the ULS labelling protocol to perform aCGH on archived FFPE leiomyosarcoma tissues using the 180k Agilent platform. Preservation age of samples ranged from a few months to seventeen years and the DNA showed a wide range of degradation (when visualised on agarose gels. Consistently high DNA labelling efficiency and low microarray probe-to-probe variation (as measured by the derivative log ratio spread was seen. Comparison of paired fresh and FFPE samples from identical tumours showed good correlation of CNAs detected. Furthermore, the ability to macro-dissect FFPE samples permitted the detection of CNAs that were masked in fresh tissue. Aberrations were visually confirmed using Fluorescence in situ Hybridisation. These results suggest that archival FFPE tissue, with its relative abundance and attendant clinical data may be used for effective mapping for genomic copy number aberrations in such rare tumours as leiomyosarcoma and potentially unravel clues to tumour origins, progression and ultimately, targeted treatment.

  19. Nanocrystalline magnetic materials obtained by flash annealing

    Directory of Open Access Journals (Sweden)

    R.K. Murakami

    1999-04-01

    Full Text Available The aim of the present work was to produce enhanced-remanence nanocrystalline magnetic material by crystallizing amorphous or partially amorphous Pr4.5Fe77B18.5 alloys by the flash annealing process, also known as the dc-Joule heating process, and to determine the optimal conditions for obtaining good magnetic coupling between the magnetic phases present in this material. Ribbons of Pr4.5Fe77B18.5 were produced by melt spinning and then annealed for 10-30 s at temperatures 500 - 640 °C by passing current through the sample to develop the enhanced-remanence nanocrystalline magnetic material. These materials were studied by X-ray diffraction, differential thermal analysis and magnetic measurements. Coercivity increases of up to 15% were systematically observed in relation to furnace-annealed material. Two different samples were carefully examined: (i a sample annealed at 600 °C which showed the highest coercive field Hc and remanence ratio Mr/Ms and (ii a sample annealed at 520 °C which showed phase separation in the second quadrant demagnetization curve. Our results are in agreement with other studies which show that flash annealing improves the magnetic properties of some amorphous ferromagnetic ribbons.

  20. Coming out in the archives: the Hall-Carpenter Archives at the London School of Economics

    OpenAIRE

    Donnelly, Sue

    2008-01-01

    The archive and journal collections of the Hall-Carpenter Archives (HCA) have been housed at the LSE since 1988. The archive, named in honour of novelist Radclyffe Hall and socialist writer, Edward Carpenter, was founded in 1982 to document the development of gay activism in the UK since the publication of the Wolfenden Report in 1958. The archive operated as an independent archive based at the London Lesbian and Gay Centre for several years before being transferred to the Archives of the Lon...

  1. RECOMMENDATIONS FOR SAMPLING OF TANK 19 IN F TANK FARM

    Energy Technology Data Exchange (ETDEWEB)

    Harris, S.; Shine, G.

    2009-12-14

    concentration as a function of the number of samples, and the final number of samples is determined when the reduction in the uncertainty from an additional sample no longer has a practical impact on results. The characterization of the full suite of analytes in the North and South hemispheres is currently supported by a single Mantis rover sample in each hemisphere. A floor scrape sample was obtained from a compact region near the center riser slightly in the South hemisphere and has been analyzed for a shortened list of key analytes. There is not enough additional material from the floor scrape sample material for completing the full suite of constituents. No floor scrape samples have been previously taken from the North hemisphere. The criterion to determine the number of additional samples was based on the practical reduction in the uncertainty when a new sample is added. This was achieved when five additional samples are obtained. In addition, two archived samples will be used if a contingency such as failing to demonstrate the comparability of the Mantis samples to the floor scrape samples occurs. To complete sampling of the Tank 19 residual floor material, four additional samples should be taken from the North hemisphere and four additional samples should be taken from the South hemisphere. One of the samples from each hemisphere will be archived in case of need. Three of the four additional samples from each hemisphere will be analyzed. Once the results are available, differences between the Mantis and three floor scrape sample results will be evaluated. If there are no statistically significant analyte concentration differences between the Mantis and floor scrape samples, those results will be combined and then UCL95%s will be calculated. If the analyte concentration differences between the Mantis and floor scrape samples are statistically significant, the UCL95%s will be calculated without the Mantis sample results. If further reduction in the upper confidence limits

  2. Researchers’ Attitudes and Behaviour to Data Archiving Policies and Practice is Typified by Low Awareness and Little Planning. A Review of: Perry, Carol Marie. “Archiving of Publicly Funded Research Data: A Survey of Canadian Researchers.” Government Information Quarterly 25 (2008: 133-48.

    Directory of Open Access Journals (Sweden)

    Gaby Haddow

    2008-12-01

    Full Text Available Objective – To assess researchers’ attitudes and behaviours in relation to archiving research data and to determine researchers’views about policies relating to data archiving.Design – Survey.Setting – Canadian universities.Subjects – Social sciences and humanities researchers from universities across Canada.Methods – A questionnaire comprising 15 questions was mailed to 175 researchers randomly sampled from a publicly available list of 5,821 individuals who had received grants and awards from the Social Sciences and Humanities Research Council of Canada (SSHRC. From this sample, 75 (43.4% responded within the five week time-frame stipulated. The questionnaire was constructed using four existing surveys and asked researchers for information about: geographical location, years of research experience, research funding sources, current plans to archive research data, awareness of archiving policies, attitude to mandated research data archiving, effect of mandatory data archiving policies on grant-seeking, attitude to making archived research data accessible, and use of research data collected by others. The questionnaire also included space for respondents to make comments. Responses to each question were analyzed, followed by a series of cross-analyses to investigate associations between findings. These cross-analyses include: whether attitudes to making data accessible differed according to length of experience, support for a national archive initiative, or agreement with a mandatory policy; and whether plans to archive research data were associated with awareness of policy, agreement with mandatory policy, or funding from government agencies other than SSHRC. Some cross-analyses were conducted between responses to questions and the comments provided by respondents. Most of the questionnaire responses were analyzed as percentages in two categories only. For example, length of service responses were presented as “up to 20years

  3. Auto-choreography: Animating Sentient Archives

    Directory of Open Access Journals (Sweden)

    Kim Satchell

    2010-03-01

    Full Text Available The stories lives tell, the narratives they follow and the spaces they embody are critical to articulating the conditions of everyday life. This resonates in the fraught relations within and between surf cultures, affluent societies and economic development. In the context of Rural Cultural Studies the analysis of coastal place allows for a combination of self-reflexive, critical and transformative modes of inquiry. This pedagogy of the coast offers innovative approaches to contemporary anthropogenic and existential challenges. For the Ecological Humanities, adjusting to living synergistically requires dialogue, ethical frameworks and care. This includes attention to the more-than human world and the non-human inhabitants with whose lives people become entangled in the mutuality of wandering paths and shared haunts. The imaginary-material architectures and geographies of these samples of coastal life, are topographies woven of spatiality and contemporary coastal writing. These contemplations attempt to sketch performative interactions from the auto-choreography animating sentient archives.

  4. Evaluation of continued storage of samples at the 325 Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    McVey, C.B.

    1996-01-01

    This report provides identification of potential Program users for samples that are archived at Pacific Northwest National Laboratory (PNNL) facilities, establishes cost releated to packaging and transportation of these samples to 222-S, for continued storage at 325 facilities, disposal and transfer costs. The report provides a recommendation to dispose of 20 samples, transfer 99 samples to PNNL and to continue storage of the remaining samples at PNNL`s 325 facility.

  5. The AMBRE Project: Parameterisation of FGK-type stars from the ESO:HARPS archived spectra

    CERN Document Server

    De Pascale, Marco; de Laverny, Patrick; Recio-Blanco, Alejandra; Hill, Vanessa; Bijaoui, Albert

    2014-01-01

    The AMBRE project is a collaboration between the European Southern Observatory (ESO) and the Observatoire de la Cote d'Azur (OCA). It has been established to determine the stellar atmospheric parameters (effective temperature, surface gravity, global metallicities and abundance of alpha-elements over iron) of the archived spectra of four ESO spectrographs. The analysis of the ESO:HARPS archived spectra is presented. The sample being analysed (AMBRE:HARPS) covers the period from 2003 to 2010 and is comprised of 126688 scientific spectra corresponding to 17218 different stars. For the analysis of the spectral sample, the automated pipeline developed for the analysis of the AMBRE:FEROS archived spectra has been adapted to the characteristics of the HARPS spectra. Within the pipeline, the stellar parameters are determined by the MATISSE algorithm, developed at OCA for the analysis of large samples of stellar spectra in the framework of galactic archaeology. In the present application, MATISSE uses the AMBRE grid ...

  6. Optimisation and validation of methods to assess single nucleotide polymorphisms (SNPs) in archival histological material

    DEFF Research Database (Denmark)

    Andreassen, C N; Sørensen, Flemming Brandt; Overgaard, J;

    2004-01-01

    TGFB1 SNPs was used to provide an indirect validation of the genotyping results. Furthermore, two different methods for DNA extraction were compared (semi-automatic DNA extraction using the ABI Prism 6100 Nucleic Acid PrepStation versus Proteinase K digestion for 5 days followed by boiling and DNA...... precipitation). RESULTS: Assessment of SNPs based on archival histological material is encumbered by a number of obstacles and pitfalls. However, these can be widely overcome by careful optimisation of the methods used for sample selection, DNA extraction and PCR. Within 130 samples that fulfil the criteria...... only archival specimens are available. This study was conducted to validate protocols optimised for assessment of SNPs based on paraffin embedded, formalin fixed tissue samples. PATIENTS AND METHODS: In 137 breast cancer patients, three TGFB1 SNPs were assessed based on archival histological specimens...

  7. Sample Design.

    Science.gov (United States)

    Ross, Kenneth N.

    1987-01-01

    This article considers various kinds of probability and non-probability samples in both experimental and survey studies. Throughout, how a sample is chosen is stressed. Size alone is not the determining consideration in sample selection. Good samples do not occur by accident; they are the result of a careful design. (Author/JAZ)

  8. Balanced sampling

    NARCIS (Netherlands)

    Brus, D.J.

    2015-01-01

    In balanced sampling a linear relation between the soil property of interest and one or more covariates with known means is exploited in selecting the sampling locations. Recent developments make this sampling design attractive for statistical soil surveys. This paper introduces balanced sampling

  9. Teacher Evaluation: Archiving Teaching Effectiveness

    Science.gov (United States)

    Nielsen, Lance D.

    2014-01-01

    Teacher evaluation is a current hot topic within music education. This article offers strategies for K-12 music educators on how to promote their effectiveness as teachers through archival documentation in a teacher portfolio. Using the Danielson evaluation model (based on four domains of effective teaching practices), examples of music teaching…

  10. Netherlands: archives, libraries and museums

    NARCIS (Netherlands)

    E. Ketelaar; F. Huysmans; P. van Mensch

    2010-01-01

    This entry provides an overview of the development and current state of archives, libraries, and museums as institutions, and the related professions and disciplines within the Netherlands. The entry describes social and political issues affecting information institutions from the early nineteenth c

  11. Archived film analysis and restoration

    NARCIS (Netherlands)

    Rares, A.

    2004-01-01

    The progressive degradation of current film archives poses a serious threat to the preservation of our cultural and technical heritage. Digitization and digital restoration are currently the most viable solutions for the long term preservation and high quality restoration of filmed material. They al

  12. Preservation and Archives in Vietnam.

    Science.gov (United States)

    Henchy, Judith

    This report, based on visits to Vietnamese libraries and archives between 1987 and 1997, examines the largely unexplored corpus of Vietnamese textual resources in research institutions and libraries there and elsewhere, the associated problems of bibliographic control, and issues of preservation. The following topics are addressed: the history of…

  13. Characterization, Leaching, and Filtration Testing for Tributyl Phosphate (TBP, Group 7) Actual Waste Sample Composites

    Energy Technology Data Exchange (ETDEWEB)

    Edwards, Matthew K.; Billing, Justin M.; Blanchard, David L.; Buck, Edgar C.; Casella, Amanda J.; Casella, Andrew M.; Crum, J. V.; Daniel, Richard C.; Draper, Kathryn E.; Fiskum, Sandra K.; Jagoda, Lynette K.; Jenson, Evan D.; Kozelisky, Anne E.; MacFarlan, Paul J.; Peterson, Reid A.; Shimskey, Rick W.; Snow, Lanee A.; Swoboda, Robert G.

    2009-03-09

    .A testing program evaluating actual tank waste was developed in response to Task 4 from the M-12 External Flowsheet Review Team (EFRT) issue response plan. The bulk water-insoluble solid wastes that are anticipated to be delivered to the Waste Treatment and Immobilization Plant (WTP) were identified according to type such that the actual waste testing could be targeted to the relevant categories. Eight broad waste groupings were defined. Samples available from the 222S archive were identified and obtained for testing. The actual waste-testing program included homogenizing the samples by group, characterizing the solids and aqueous phases, and performing parametric leaching tests. The tributyl phosphate sludge (TBP, Group 7) is the subject of this report. The Group 7 waste was anticipated to be high in phosphorus as well as aluminum in the form of gibbsite. Both are believed to exist in sufficient quantities in the Group 7 waste to address leaching behavior. Thus, the focus of the Group 7 testing was on the removal of both P and Al. The waste-type definition, archived sample conditions, homogenization activities, characterization (physical, chemical, radioisotope, and crystal habit), and caustic leaching behavior as functions of time, temperature, and hydroxide concentration are discussed in this report. Testing was conducted according to TP-RPP-WTP-467.

  14. Archives of Atmospheric Lead Pollution

    Science.gov (United States)

    Weiss, Dominik; Shotyk, William; Kempf, Oliver

    Environmental archives such as peat bogs, sediments, corals, trees, polar ice, plant material from herbarium collections, and human tissue material have greatly helped to assess both ancient and recent atmospheric lead deposition and its sources on a regional and global scale. In Europe detectable atmospheric lead pollution began as early as 6000years ago due to enhanced soil dust and agricultural activities, as studies of peat bogs reveal. Increased lead emissions during ancient Greek and Roman times have been recorded and identified in many long-term archives such as lake sediments in Sweden, ice cores in Greenland, and peat bogs in Spain, Switzerland, the United Kingdom, and the Netherlands. For the period since the Industrial Revolution, other archives such as corals, trees, and herbarium collections provide similar chronologies of atmospheric lead pollution, with periods of enhanced lead deposition occurring at the turn of the century and since 1950. The main sources have been industry, including coal burning, ferrous and nonferrous smelting, and open waste incineration until c.1950 and leaded gasoline use since 1950. The greatest lead emissions to the atmosphere all over Europe occurred between 1950 and 1980 due to traffic exhaust. A marked drop in atmospheric lead fluxes found in most archives since the 1980s has been attributed to the phasing out of leaded gasoline. The isotope ratios of lead in the various archives show qualitatively similar temporal changes, for example, the immediate response to the introduction and phasing out of leaded gasoline. Isotope studies largely confirm source assessments based on lead emission inventories and allow the contributions of various anthropogenic sources to be calculated.

  15. Body weight - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open...d License Update History of This Database Site Policy | Contact Us Body weight - Open TG-GATEs | LSDB Archive ...

  16. Cell viability - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open... Database Description Download License Update History of This Database Site Policy | Contact Us Cell viability - Open TG-GATEs | LSDB Archive ...

  17. Pathological items - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open... This Database Site Policy | Contact Us Pathological items - Open TG-GATEs | LSDB Archive ...

  18. Individual list - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open... History of This Database Site Policy | Contact Us Individual list - Open TG-GATEs | LSDB Archive ...

  19. Biochemistry - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open...e Database Description Download License Update History of This Database Site Policy | Contact Us Biochemistry - Open TG-GATEs | LSDB Archive ...

  20. Organ weight - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open...load License Update History of This Database Site Policy | Contact Us Organ weight - Open TG-GATEs | LSDB Archive ...

  1. Hematology - Open TG-GATEs | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Open...base Description Download License Update History of This Database Site Policy | Contact Us Hematology - Open TG-GATEs | LSDB Archive ...

  2. A Critical Archival Pedagogy: The Lesbian Herstory Archives and a Course in Radical Lesbian Thought

    Directory of Open Access Journals (Sweden)

    Kailah R. Carden

    2016-07-01

    Full Text Available This paper is the story of a critical archival pedagogy that emerged through the undergraduate course Radical Lesbian Thought. As teachers and students, we dialogically co-constructed the praxis and content of the course throughout the semester. We employed archives throughout the course as theory, site, and pedagogy. In this paper we identify three archival frameworks: dialogue and difference, collaborative knowledge production, and archival methodology; and detail how they informed three course activities: reading and writing archival letters, visiting the Lesbian Herstory Archives, and completing final archival projects. We argue that archives provide theoretical and practical opportunities, in the tradition of critical pedagogy, to challenge and rearrange powered classroom structures and practices of thought.

  3. Image File - TP Atlas | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us TP Atlas...wnload License Update History of This Database Site Policy | Contact Us Image File - TP Atlas | LSDB Archive ...

  4. About Libraries - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ry.zip File URL: ftp://ftp.biosciencedbc.jp/archive/acest/LATEST/acest_library.zi...p File size: 2KB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/archive_acest_library#en Data ...ription Download License Update History of This Database Site Policy | Contact Us About Libraries - AcEST | LSDB Archive ...

  5. Amino Acid Sequence - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ll_sequence_amino_db.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST...ta.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_ine_full_sequence_amino_db.fasta.zip Fi...date History of This Database Site Policy | Contact Us Amino Acid Sequence - KOME | LSDB Archive ...

  6. AcEST Contig - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...contig.zip File URL: ftp://ftp.biosciencedbc.jp/archive/acest/LATEST/acestcontig....zip File size: 1.34MB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/archive_acestcontig#en Da...Joomla SEF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us AcEST Contig - AcEST | LSDB Archive ...

  7. The Sloan Digital Sky Survey and its Archive

    CERN Document Server

    Szalay, A S; Thakar, A R; Gray, J; Slutz, D; Szalay, Alexander S.; Kunszt, Peter; Thakar, Anirudha; Gray, Jim; Slutz, Don

    1999-01-01

    The next-generation astronomy archives will cover most of the universe at fine resolution in many wavelengths. One of the first of these projects, the Sloan Digital Sky Survey (SDSS) will create a 5-wavelength catalog over 10,000 square degrees of the sky. The 200 million objects in the multi-terabyte database will have mostly numerical attributes, defining a space of 100+ dimensions. Points in this space have highly correlated distributions. The archive will enable astronomers to explore the data interactively. Data access will be aided by multidimensional spatial indices. The data will be partitioned in many ways. Small tag objects consisting of the most popular attributes speed up frequent searches. Splitting the data among multiple servers enables parallel, scalable I/O. Hashing techniques allow efficient clustering and pairwise comparison algorithms. Randomly sampled subsets allow debugging otherwise large queries at the desktop. Central servers will operate a data pump that supports sweeping searches th...

  8. SOUSA: the Swift Optical/Ultraviolet Supernova Archive

    CERN Document Server

    Brown, Peter J; Holland, Stephen; Kuin, Paul; Pritchard, Tyler

    2014-01-01

    The Ultra-Violet Optical Telescope on the Swift spacecraft has observed hundreds of supernovae, covering all major types and most subtypes. Here we introduce the Swift Optical/Ultraviolet Supernova Archive (SOUSA), which will contain all of the supernova images and photometry. We describe the observation and reduction procedures and how they impact the final data. We show photometry from well-observed examples of most supernova classes, whose absolute magnitudes and colors may be used to infer supernova types in the absence of a spectrum. A full understanding of the variety within classes and a robust photometric separation of the groups requires a larger sample, which will be provided by the final archive. The data from the existing Swift supernovae are also useful for planning future observations with Swift as well as future UV observatories.

  9. NetCDF based data archiving system applied to ITER Fast Plant System Control prototype

    Energy Technology Data Exchange (ETDEWEB)

    Castro, R., E-mail: rodrigo.castro@visite.es [Asociacion EURATOM/CIEMAT para Fusion, Madrid (Spain); Vega, J. [Asociacion EURATOM/CIEMAT para Fusion, Madrid (Spain); Ruiz, M.; De Arcas, G.; Barrera, E.; Lopez, J.M.; Sanz, D. [Grupo de Investigacion en Instrumentacion y Acustica Aplicada, UPM, Madrid (Spain); Goncalves, B.; Santos, B. [Associacao EURATOM/IST, IPFN - Laboratorio Associado, IST, Lisboa (Portugal); Utzel, N.; Makijarvi, P. [ITER Organization, St. Paul lez Durance Cedex (France)

    2012-12-15

    Highlights: Black-Right-Pointing-Pointer Implementation of a data archiving solution for a Fast Plant System Controller (FPSC) for ITER CODAC. Black-Right-Pointing-Pointer Data archiving solution based on scientific NetCDF-4 file format and Lustre storage clustering. Black-Right-Pointing-Pointer EPICS control based solution. Black-Right-Pointing-Pointer Tests results and detailed analysis of using NetCDF-4 and clustering technologies on fast acquisition data archiving. - Abstract: EURATOM/CIEMAT and Technical University of Madrid (UPM) have been involved in the development of a FPSC (Fast Plant System Control) prototype for ITER, based on PXIe (PCI eXtensions for Instrumentation). One of the main focuses of this project has been data acquisition and all the related issues, including scientific data archiving. Additionally, a new data archiving solution has been developed to demonstrate the obtainable performances and possible bottlenecks of scientific data archiving in Fast Plant System Control. The presented system implements a fault tolerant architecture over a GEthernet network where FPSC data are reliably archived on remote, while remaining accessible to be redistributed, within the duration of a pulse. The storing service is supported by a clustering solution to guaranty scalability, so that FPSC management and configuration may be simplified, and a unique view of all archived data provided. All the involved components have been integrated under EPICS (Experimental Physics and Industrial Control System), implementing in each case the necessary extensions, state machines and configuration process variables. The prototyped solution is based on the NetCDF-4 (Network Common Data Format) file format in order to incorporate important features, such as scientific data models support, huge size files management, platform independent codification, or single-writer/multiple-readers concurrency. In this contribution, a complete description of the above mentioned solution

  10. System Architecture for a Hubble Legacy Archive

    Science.gov (United States)

    Miller, W. W., III; Gaffney, N.; Jenkner, H.; Whitmore, B.

    2007-10-01

    The Space Telescope Science Institute (STScI), in collaboration with the European Coordinating Facility (ECF) and the Canadian Astronomy Data Centre (CADC), is studying the development of an enhanced archive for the Hubble Space Telescope (HST). Distinct from (but complimentary to) the existing archive system at the STScI, development of this enhanced archive affords the opportunity to leverage modern architectural and design principles toward meeting project objectives and serving the archive community. To wit, the STScI is designing the entire system as a service-oriented architecture (SOA). Composed of loosely-coupled, highly-interoperable Web services, an SOA embodies a great deal of flexibility in meeting future needs placed on the HST archive while also positioning it well within the emerging Virtual Observatory (VO) framework. A high-level architecture for the enhanced archive at the STScI is presented.

  11. Science in the archives pasts, presents, futures

    CERN Document Server

    2017-01-01

    "Science in the Archives" reveals affinities and continuities among the sciences of the archives, across many disciplines and centuries, in order to present a better picture of essential archival practices and, thereby, the meaning of science. For in both the natural and human sciences, archives of the most diverse forms make cumulative, collective knowledge possible. Yet in contrast to laboratories, observatories, or the field, archives have yet to be studied across the board as central sites of science. The volume covers episodes in the history of astronomy, geology, genetics, classical philology, climatology, history, medicine, and ancient natural philosophy, as well as fundamental practices such as collecting, retrieval strategies, and data mining. The time frame spans doxology in Greco-Roman antiquity to NSA surveillance techniques and the quantified-self movement. Each chapter explores the practices, politics, economics, and open-ended potential of the sciences of the archives, making this the first boo...

  12. Web Services interface of SSRF archive data analysis system

    Institute of Scientific and Technical Information of China (English)

    LI Lin; SHEN Liren; ZHU Qing; WAN Tianmin

    2007-01-01

    Accelerator database stores various static parameters and real-time data of accelerator. SSRF (Shanghai Synchrotron Radiation Facility) adopts relational database to save the data. We developed a data retrieval system based on XML Web Services for accessing the archive data. It includes a bottom layer interface and an interface applicable for accelerator physics. Client samples exemplifying how to consume the interface are given. The users can browse, retrieve and plot data by the client samples. Also, we give a method to test its stability. The test result and performance are described.

  13. Mapping data detail - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ct (IRGSP) Data file File name: kome_mapping_data_detail.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...istory of This Database Site Policy | Contact Us Mapping data detail - KOME | LSDB Archive ...

  14. Tos17 link result - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...s17 mutant clones Data file File name: kome_tos17_link_result.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...te History of This Database Site Policy | Contact Us Tos17 link result - KOME | LSDB Archive ...

  15. PLACE search result - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ce_search_result.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kom...n Download License Update History of This Database Site Policy | Contact Us PLACE search result - KOME | LSDB Archive ...

  16. Memsat search result - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ... transmembrane region predictions Data file File name: kome_memsat_search_result.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...This Database Database Description Download License Update History of This Database Site Policy | Contact Us Memsat search result - KOME | LSDB Archive ...

  17. tRNA - RMG | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...genomes of other plants. Data file File name: rmg_trna.zip File URL: ftp://ftp.biosciencedbc.jp/archive/rmg/...cription Download License Update History of This Database Site Policy | Contact Us tRNA - RMG | LSDB Archive ...

  18. ORF Table - KAIKOcDNA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ... of the representative ESTs. Data file File name: kaiko_cdna_orf.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...istory of This Database Site Policy | Contact Us ORF Table - KAIKOcDNA | LSDB Archive ...

  19. RPD link result - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...searches against the Rice Proteome Database Data file File name: kome_rpd_link_result.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...ry of This Database Site Policy | Contact Us RPD link result - KOME | LSDB Archive ...

  20. Update History of This Database - KEGG MEDICUS | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us KEGG MEDICUS Update... History of This Database Date Update contents 2014/05/09 KEGG MEDICUS English archive si...io About This Database Database Description Download License Update History of Th...is Database Site Policy | Contact Us Update History of This Database - KEGG MEDICUS | LSDB Archive ...