WorldWideScience

Sample records for approved application blood

  1. 78 FR 32668 - Draft Guidance for Industry: Changes to an Approved Application: Biological Products: Human Blood...

    Science.gov (United States)

    2013-05-31

    ...The Food and Drug Administration (FDA) is announcing the availability of a draft document entitled ``Guidance for Industry: Changes to an Approved Application: Biological Products: Human Blood and Blood Components Intended for Transfusion or for Further Manufacture'' dated June 2013. The draft guidance document provides manufacturers of licensed Whole Blood and blood components intended for......

  2. 21 CFR 314.125 - Refusal to approve an application.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) DRUGS FOR HUMAN USE APPLICATIONS FOR FDA APPROVAL TO MARKET A NEW DRUG FDA Action on Applications... study that is described in the application and that is essential to show that the drug is safe for use... adequately protected. (17) The applicant or contract research organization that conducted a...

  3. 21 CFR 314.105 - Approval of an application and an abbreviated application.

    Science.gov (United States)

    2010-04-01

    ... labeling prior to marketing. (c) FDA will approve an application after it determines that the drug meets... not be introduced or delivered for introduction into interstate commerce until approval of...

  4. 21 CFR 314.153 - Suspension of approval of an abbreviated new drug application.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 5 2010-04-01 2010-04-01 false Suspension of approval of an abbreviated new drug... HUMAN SERVICES (CONTINUED) DRUGS FOR HUMAN USE APPLICATIONS FOR FDA APPROVAL TO MARKET A NEW DRUG FDA... new drug application. (a) Suspension of approval. The approval of an abbreviated new drug...

  5. 21 CFR 314.127 - Refusal to approve an abbreviated new drug application.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 5 2010-04-01 2010-04-01 false Refusal to approve an abbreviated new drug... HUMAN SERVICES (CONTINUED) DRUGS FOR HUMAN USE APPLICATIONS FOR FDA APPROVAL TO MARKET A NEW DRUG FDA Action on Applications and Abbreviated Applications § 314.127 Refusal to approve an abbreviated new...

  6. 75 FR 55334 - Schmid Laboratories, Inc. et al.; Withdrawal of Approval of Five New Drug Applications

    Science.gov (United States)

    2010-09-10

    ... Five New Drug Applications AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) is withdrawing approval of five new drug applications (NDAs)...

  7. 75 FR 71135 - Hoffmann-La Roche Inc.; Withdrawal of Approval of a New Drug Application

    Science.gov (United States)

    2010-11-22

    ... HUMAN SERVICES Food and Drug Administration Hoffmann-La Roche Inc.; Withdrawal of Approval of a New Drug... Administration (FDA) is withdrawing approval of a new drug application (NDA) for ACCUTANE (isotretinoin) Capsules... be marketed under approved abbreviated new drug applications (ANDAs). The holders of ANDAs...

  8. 30 CFR 18.81 - Field modification of approved (permissible) equipment; application for approval of modification...

    Science.gov (United States)

    2010-07-01

    ... TESTING, EVALUATION, AND APPROVAL OF MINING PRODUCTS ELECTRIC MOTOR-DRIVEN MINE EQUIPMENT AND ACCESSORIES Machines Assembled With Certified or Explosion-Proof Components, Field Modifications of Approved Machines... and Certification Center, 765 Technology Drive, Triadelphia, WV 26059. (b) Proposed...

  9. 21 CFR 314.150 - Withdrawal of approval of an application or abbreviated application.

    Science.gov (United States)

    2010-04-01

    ... HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS FOR HUMAN USE APPLICATIONS FOR FDA APPROVAL TO MARKET A NEW... that is described in the application or abbreviated application and that is essential to show that the... or contract research organization that conducted a bioavailability or bioequivalence study...

  10. 21 CFR 314.152 - Notice of withdrawal of approval of an application or abbreviated application for a new drug.

    Science.gov (United States)

    2010-04-01

    ... or abbreviated application for a new drug. 314.152 Section 314.152 Food and Drugs FOOD AND DRUG... APPROVAL TO MARKET A NEW DRUG FDA Action on Applications and Abbreviated Applications § 314.152 Notice of withdrawal of approval of an application or abbreviated application for a new drug. If the Food and...

  11. 21 CFR 900.3 - Application for approval as an accreditation body.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Application for approval as an accreditation body... approval as an accreditation body. (a) Eligibility. Private nonprofit organizations or State agencies capable of meeting the requirements of this subpart A may apply for approval as accreditation bodies....

  12. 78 FR 70566 - Withdrawal of Approval of New Animal Drug Applications; Arsanilic Acid

    Science.gov (United States)

    2013-11-26

    ... withdrawing approval of a new animal drug application (NADA) for an arsanilic acid Type A medicated article at... 34384, Charlotte, NC 28234 has requested that FDA withdraw approval of NADA 008-019 for PRO-GEN... approval of NADA 008-019, and all supplements and amendments thereto, is hereby withdrawn. Elsewhere...

  13. 78 FR 70496 - Withdrawal of Approval of New Animal Drug Applications; Arsanilic Acid

    Science.gov (United States)

    2013-11-26

    ... approval of a new animal drug application (NADA) for an arsanilic acid Type A medicated article at the.... Box 34384, Charlotte, NC 28234 has requested that FDA withdraw approval of NADA 008-019 for PRO-GEN... gave notice that approval of NADA 008-019, and all supplements and amendments thereto, is...

  14. 77 FR 50702 - Ranbaxy Laboratories Limited; Withdrawal of Approval of 27 Abbreviated New Drug Applications

    Science.gov (United States)

    2012-08-22

    ... Abbreviated New Drug Applications AGENCY: Food and Drug Administration, HHS. ACTION: Notice. ] SUMMARY: The Food and Drug Administration (FDA) is withdrawing approval of 27 abbreviated new drug applications... introduction into interstate commerce of products without approved new drug applications violates section...

  15. Blood drop patterns: Formation and applications.

    Science.gov (United States)

    Chen, Ruoyang; Zhang, Liyuan; Zang, Duyang; Shen, Wei

    2016-05-01

    The drying of a drop of blood or plasma on a solid substrate leads to the formation of interesting and complex patterns. Inter- and intra-cellular and macromolecular interactions in the drying plasma or blood drop are responsible for the final morphologies of the dried patterns. Changes in these cellular and macromolecular components in blood caused by diseases have been suspected to cause changes in the dried drop patterns of plasma and whole blood, which could be used as simple diagnostic tools to identify the health of humans and livestock. However, complex physicochemical driving forces involved in the pattern formation are not fully understood. This review focuses on the scientific development in microscopic observations and pattern interpretation of dried plasma and whole blood samples, as well as the diagnostic applications of pattern analysis. Dried drop patterns of plasma consist of intricate visible cracks in the outer region and fine structures in the central region, which are mainly influenced by the presence and concentration of inorganic salts and proteins during drying. The shrinkage of macromolecular gel and its adhesion to the substrate surface have been thought to be responsible for the formation of the cracks. Dried drop patterns of whole blood have three characteristic zones; their formation as functions of drying time has been reported in the literature. Some research works have applied engineering treatment to the evaporation process of whole blood samples. The sensitivities of the resultant patterns to the relative humidity of the environment, the wettability of the substrates, and the size of the drop have been reported. These research works shed light on the mechanisms of spreading, evaporation, gelation, and crack formation of the blood drops on solid substrates, as well as on the potential applications of dried drop patterns of plasma and whole blood in diagnosis.

  16. 75 FR 75548 - SI Financial Group, Inc., Willimantic, CT; Approval of Conversion Application

    Science.gov (United States)

    2010-12-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision SI Financial Group, Inc., Willimantic, CT; Approval of Conversion Application Notice is hereby given that on November 10, 2010, the Office of Thrift Supervision approved the application of SI Bancorp, MHC,...

  17. 75 FR 52065 - SharePlus Federal Bank, Plano, TX; Approval of Conversion Application

    Science.gov (United States)

    2010-08-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision SharePlus Federal Bank, Plano, TX; Approval of Conversion Application Notice is hereby given that on August 12, 2010, the Office of Thrift Supervision approved the application...

  18. 42 CFR 8.3 - Application for approval as an accreditation body.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Application for approval as an accreditation body... accreditation body. (a) Eligibility. Private nonprofit organizations or State governmental entities, or... an accreditation body. (b) Application for initial approval. Three copies of an accreditation...

  19. 75 FR 80061 - Abbott Laboratories, Inc.; Withdrawal of Approval of a New Drug Application for MERIDIA

    Science.gov (United States)

    2010-12-21

    ... HUMAN SERVICES Food and Drug Administration Abbott Laboratories, Inc.; Withdrawal of Approval of a New Drug Application for MERIDIA AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) is withdrawing approval of a new drug application (NDA) for...

  20. 5 CFR 630.905 - Approval of application to become a leave recipient.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Approval of application to become a leave recipient. 630.905 Section 630.905 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS ABSENCE AND LEAVE Voluntary Leave Transfer Program § 630.905 Approval of application to become...

  1. 5 CFR 630.1007 - Approval of application to become a leave recipient.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Approval of application to become a leave recipient. 630.1007 Section 630.1007 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS ABSENCE AND LEAVE Voluntary Leave Bank Program § 630.1007 Approval of application to become...

  2. 24 CFR 886.107 - Approval of applications.

    Science.gov (United States)

    2010-04-01

    ...) The Owner's Affirmative Fair Housing Marketing Plan is approvable. (b) The HUD-approved unit rents are.... Occupancy requirements are being met. Marketing and maintenance programs are being carried out in an... collection losses. (7) The Owner's plan for remedying any deferred maintenance, financial problems, or...

  3. 78 FR 78796 - Supplemental Applications Proposing Labeling Changes for Approved Drugs and Biological Products...

    Science.gov (United States)

    2013-12-27

    ... Applications Proposing Labeling Changes for Approved Drugs and Biological Products; Correction and Extension of... holders of an approved drug or biological product to change the product labeling to reflect certain types... biological product to change the product labeling to reflect certain types of newly acquired information...

  4. 77 FR 50121 - Hospira, Inc.; Withdrawal of Approval of a New Drug Application for DEXTRAN 70

    Science.gov (United States)

    2012-08-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Hospira, Inc.; Withdrawal of Approval of a New Drug... Drug Administration (FDA) is withdrawing approval of a new drug application (NDA) for DEXTRAN 70...

  5. 78 FR 46984 - Pfizer, Inc.; Withdrawal of Approval of a New Drug Application for BEXTRA

    Science.gov (United States)

    2013-08-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Pfizer, Inc.; Withdrawal of Approval of a New Drug... Administration (FDA) is withdrawing approval of a new drug application (NDA) for BEXTRA (valdecoxib) 10...

  6. 75 FR 65642 - Withdrawal of Approval of New Animal Drug Applications; Aklomide; Levamisole Hydrochloride...

    Science.gov (United States)

    2010-10-26

    ...The Food and Drug Administration (FDA) is withdrawing approval of eight new animal drug applications (NADAs). In a final rule published elsewhere in this issue of the Federal Register, FDA is amending the animal drug regulations to remove portions reflecting approval of these...

  7. 21 CFR 515.20 - Approval of medicated feed mill license applications.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Approval of medicated feed mill license... SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS MEDICATED FEED MILL LICENSE Administrative Actions on Licenses § 515.20 Approval of medicated feed mill license applications. Within 90 days after...

  8. 21 CFR 515.21 - Refusal to approve a medicated feed mill license application.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Refusal to approve a medicated feed mill license... SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS MEDICATED FEED MILL LICENSE Administrative Actions on Licenses § 515.21 Refusal to approve a medicated feed mill license application. (a)...

  9. 34 CFR 668.144 - Application for test approval.

    Science.gov (United States)

    2010-07-01

    ... distribution of test scores for each edition, form, level, sub-test, or partial battery, for which approval is....147; (5) Documentation of test development, including a history of the test's use; (6) Norming...

  10. 76 FR 22713 - Withdrawal of Approval of New Animal Drug Applications; Phenylbutazone; Pyrantel; Tylosin...

    Science.gov (United States)

    2011-04-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Withdrawal of Approval of New Animal Drug Applications; Phenylbutazone; Pyrantel; Tylosin; Sulfamethazine; Correction AGENCY: Food and Drug Administration, HHS....

  11. 78 FR 36641 - Proposed Information Collection (Application for Approval of a Licensing or Certification and...

    Science.gov (United States)

    2013-06-18

    ... AFFAIRS Proposed Information Collection (Application for Approval of a Licensing or Certification and Organization or Entity) Activity: Comment Request AGENCY: Veterans Benefits Administration, Department of Veterans Affairs. ACTION: Notice. SUMMARY: The Veterans Benefits Administration (VBA), Department...

  12. 75 FR 47680 - Proposed Information Collection (Application for Approval of a Licensing or Certification and...

    Science.gov (United States)

    2010-08-06

    ... AFFAIRS Proposed Information Collection (Application for Approval of a Licensing or Certification and Organization or Entity) Activity: Comment Request AGENCY: Veterans Benefits Administration, Department of Veterans Affairs. ACTION: Notice. SUMMARY: The Veterans Benefits Administration (VBA), Department...

  13. 78 FR 2295 - Consideration of Approval of Application Containing Sensitive Unclassified Non-Safeguards...

    Science.gov (United States)

    2013-01-10

    ... From the Federal Register Online via the Government Publishing Office ] NUCLEAR REGULATORY COMMISSION Consideration of Approval of Application Containing Sensitive Unclassified Non-Safeguards Information Regarding Proposed Energy Future Holdings Corporation Internal Restructuring AGENCY:...

  14. 78 FR 66748 - Smith Miller and Patch Inc. et al.; Proposal to Withdraw Approval of 14 New Drug Applications...

    Science.gov (United States)

    2013-11-06

    ... Approval of 14 New Drug Applications; Opportunity for a Hearing AGENCY: Food and Drug Administration, HHS... hearing on the Agency's proposal to withdraw approval of 14 new drug applications (NDAs) from multiple... to remove the products from the market. Any new drug product marketed without an approved new...

  15. Noncontact blood perfusion mapping in clinical applications

    Science.gov (United States)

    Iakovlev, Dmitry; Dwyer, Vincent; Hu, Sijung; Silberschmidt, Vadim

    2016-04-01

    Non-contact imaging photoplethysmography (iPPG) to detect pulsatile blood microcirculation in tissue has been selected as a successor to low spatial resolution and slow scanning blood perfusion techniques currently employed by clinicians. The proposed iPPG system employs a novel illumination source constructed of multiple high power LEDs with narrow spectral emission, which are temporally modulated and synchronised with a high performance sCMOS sensor. To ensure spectrum stability and prevent thermal wavelength drift due to junction temperature variations, each LED features a custom-designed thermal management system to effectively dissipate generated heat and auto-adjust current flow. The use of a multi-wavelength approach has resulted in simultaneous microvascular perfusion monitoring at various tissue depths, which is an added benefit for specific clinical applications. A synchronous detection algorithm to extract weak photoplethysmographic pulse-waveforms demonstrated robustness and high efficiency when applied to even small regions of 5 mm2. The experimental results showed evidences that the proposed system could achieve noticeable accuracy in blood perfusion monitoring by creating complex amplitude and phase maps for the tissue under examination.

  16. 10 CFR 725.15 - Requirements for approval of applications.

    Science.gov (United States)

    2010-01-01

    ... engaged in a substantial effort to develop, design, build or operate a chemical processing plant or other... Secret Restricted Data in C-91, Nuclear Reactors for Rocket Propulsion, will be approved only if the... capable of making a contribution to research and development in the field of nuclear reactors for...

  17. Blood hero: An application for encouraging the blood donation by applying gamification.

    Science.gov (United States)

    Domingos, Daniela C L; Lima, Luis F S G; Messias, Thiago F; Feijo, Jose V L; Diniz, Anthony A R; Soares, Heliana B

    2016-08-01

    There is a strong need for actions to supply the blood demand in the World. Based on this fact, it was designed an application, named `Blood Hero', by applying the `gamification' concept, which allows users to be rewarded by social acts related to the blood donation. It takes advantage of the application of mobile devices, implementing a specific social network, to attract and retain blood donators. This application makes possible an interaction between users and blood centers, and is being tested aiming at evaluating its acceptance and impact in bloodstocks.

  18. The Global Application of ISBT 128 in Blood Transfusion

    Institute of Scientific and Technical Information of China (English)

    Paul Ashford

    2006-01-01

    @@ Blood Transfusion is a global activity, both in terms of its widespread application, and because of the need in specific circumstances for blood to move across organizational and national boundaries in its journey from donor to patient.

  19. 76 FR 79701 - Bristol-Myers Squibb Co. et al.; Withdrawal of Approval of 70 New Drug Applications and 97...

    Science.gov (United States)

    2011-12-22

    ... 70 New Drug Applications and 97 Abbreviated New Drug Applications; Correction AGENCY: Food and Drug... withdrawal of approval of 70 new drug applications (NDAs) and 97 abbreviated new drug applications...

  20. 75 FR 28815 - Application and Re-certification Packages for Approval of Nonprofit Organizations in FHA Activities

    Science.gov (United States)

    2010-05-24

    ... URBAN DEVELOPMENT Application and Re-certification Packages for Approval of Nonprofit Organizations in... public comments on the subject proposal. In general, a nonprofit organization must be HUD-approved and meet specific requirements to maintain approval and remain on the Nonprofit Organization Roster...

  1. 78 FR 78716 - Withdrawal of Approval of New Animal Drug Applications; Roxarsone

    Science.gov (United States)

    2013-12-27

    ... Applications; Roxarsone AGENCY: Food and Drug Administration, HHS. ACTION: Final rule. SUMMARY: The Food and... five new animal drug applications (NADAs) for roxarsone oral dosage form products at the sponsor's... requested that FDA withdraw approval of the following five NADAs for roxarsone oral dosage form...

  2. 49 CFR 107.402 - Application for designation as an approval or certification agency.

    Science.gov (United States)

    2010-10-01

    ... organization or person seeking designation as an approval or certification agency shall apply in writing to the... designation must be in English and include the following information: (1) Name and address of the applicant... with § 105.40 to serve as agent for service of process. (2) If the applicant's principal place...

  3. 27 CFR 479.64 - Procedure for approval of application.

    Science.gov (United States)

    2010-04-01

    ..., FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND CERTAIN OTHER FIREARMS Tax on Making Firearms Application to Make A Firearm § 479.64 Procedure...

  4. 78 FR 19729 - Wild Bird Conservation Act; Receipt of Application for Approval

    Science.gov (United States)

    2013-04-02

    ... Fish and Wildlife Service Wild Bird Conservation Act; Receipt of Application for Approval AGENCY: Fish... certain activities with birds that are protected in accordance with the Wild Bird Conservation Act of 1992... activities with bird species covered under the Wild Bird Conservation Act of 1992. This notice is...

  5. 75 FR 27119 - ViewPoint Financial Group, Inc., Plano, Texas; Approval of Conversion Application

    Science.gov (United States)

    2010-05-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision ViewPoint Financial Group, Inc., Plano, Texas; Approval of Conversion... application of ViewPoint MHC and ViewPoint Bank, Plano, Texas, to convert to the stock form of...

  6. 78 FR 70062 - Withdrawal of Approval of New Animal Drug Applications; Carbarsone; Roxarsone

    Science.gov (United States)

    2013-11-22

    ...; Carbarsone; Roxarsone AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) is withdrawing approval of three new animal drug applications (NADAs) for roxarsone or... longer manufactured or marketed: NADA 007-891 for 3-NITRO (roxarsone) Type A medicated article, NADA...

  7. 75 FR 9276 - Harvard Illinois Bancorp, Inc., Harvard, Illinois; Approval of Conversion Application

    Science.gov (United States)

    2010-03-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision Harvard Illinois Bancorp, Inc., Harvard, Illinois; Approval of Conversion... application of Harvard Savings Bank, Harvard, Illinois, to convert to the stock form of organization....

  8. 76 FR 46820 - Proposal To Refuse To Approve a Supplemental New Drug Application for Bromday (Bromfenac...

    Science.gov (United States)

    2011-08-03

    ... HUMAN SERVICES Food and Drug Administration Proposal To Refuse To Approve a Supplemental New Drug Application for Bromday (Bromfenac Ophthalmic Solution), 0.09%; Opportunity for a Hearing AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA), the...

  9. 7 CFR 1485.14 - Application approval and formation of agreements.

    Science.gov (United States)

    2010-01-01

    ... resources, approve those applications which it considers to present the best opportunity for developing...) Export volume and value and market share goals in each country; (v) Description of evaluation plan and suitability of the plan for performance measurement; and (vi) Past program results and evaluations,...

  10. 76 FR 27753 - American Eagle Savings Bank, Boothwyn, PA; Approval of Conversion Application

    Science.gov (United States)

    2011-05-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision American Eagle Savings Bank, Boothwyn, PA; Approval of Conversion... application of American Eagle Savings Bank, Boothwyn, Pennsylvania, to convert to the stock form...

  11. 77 FR 39983 - Migratory Bird Hunting; Application for Approval of Fluoropolymeric Shot Coatings as Nontoxic for...

    Science.gov (United States)

    2012-07-06

    ... Fish and Wildlife Service 50 CFR Part 20 RIN 1018-AY66 Migratory Bird Hunting; Application for Approval... INFORMATION CONTACT: George Allen, at 703-358-1825. SUPPLEMENTARY INFORMATION: Background The Migratory Bird Treaty Act of 1918 (Act) (16 U.S.C. 703-712 and 16 U.S.C. 742 a-j) implements migratory bird...

  12. 77 FR 66607 - Placer County Water Agency; Notice of Application for Approval of Contract for the Sale of Power...

    Science.gov (United States)

    2012-11-06

    ... Energy Regulatory Commission Placer County Water Agency; Notice of Application for Approval of Contract for the Sale of Power for a Period Extending Beyond the Term of the License Take notice that on July 17, 2012, Placer County Water Agency filed with the Commission an application for approval of:...

  13. 76 FR 78530 - Applications for Food and Drug Administration Approval To Market a New Drug; Revision of...

    Science.gov (United States)

    2011-12-19

    ... Administration Approval To Market a New Drug; Revision of Postmarketing Reporting Requirements-- Discontinuance... concern that, although the Orange Book lists all drug products with approved new drug applications (NDA) and abbreviated new drug applications (ANDA), it is not possible to determine whether the...

  14. 77 FR 24724 - Sanofi-aventis, U.S., LLC; Withdrawal of Approval of a New Drug Application for OFORTA

    Science.gov (United States)

    2012-04-25

    ... HUMAN SERVICES Food and Drug Administration Sanofi-aventis, U.S., LLC; Withdrawal of Approval of a New Drug Application for OFORTA AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) is withdrawing approval of a new drug application (NDA) for...

  15. 21 CFR 514.111 - Refusal to approve an application.

    Science.gov (United States)

    2010-04-01

    ... evaluate the safety of such drugs, are appropriate for the use of animal experimentation data; and (iv... (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS NEW ANIMAL DRUG APPLICATIONS Administrative Actions on... new animal drug, that: (1) The reports of investigations required to be submitted pursuant to...

  16. 77 FR 24723 - AstraZeneca Pharmaceuticals LP; Withdrawal of Approval of a New Drug Application for IRESSA

    Science.gov (United States)

    2012-04-25

    ... New Drug Application for IRESSA AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) is withdrawing approval of a new drug application (NDA)...

  17. Magnetic resonance angiography with blood-pool contrast agents: future applications

    Energy Technology Data Exchange (ETDEWEB)

    Fink, C. [Univ. Hospitals, Grosshadern, Munich (Germany); Goyen, M. [Univ. Medical Center, Hamburg-Eppendorf, Hamburg (Germany); Lotz, J. [Hannover Medical School, Hannover (Germany)

    2007-03-15

    Blood pool agents remain in the intravascular space for a longer time period. Therefore the optimal imaging window for vascular structures is widened to about 30 minutes. Gadofosveset trisodium (Vasovist, Bayer Schering Pharma AG, Berlin, Germany) is the first blood-pool contrast agent approved in Europe for contrast-enhanced magnetic resonance angiography (MRA) of vessels in the abdomen, pelvis and lower extremity in adults. Other possible applications of blood-pool agents are now being considered, such as assessment of venous thromboembolism, coronary artery disease or sinus venous thrombosis. Perfusion MR imaging holds promise for detecting lung perfusion defects with higher spatial resolution and reduced scan time compared with radionuclide scintigraphy. In coronary artery disease, blood-pool agents enable a substantial increase in the quality of coronary artery imaging. Quantitative myocardial perfusion and myocardial viability seem to be possible, although modifications in protocols and sequence design are necessary for optimal results. Other novel applications of blood-pool agents include monitoring of inflammatory changes in systemic lupus erythematosus and evaluation of tumour invasion into lymph nodes and more reliable assessment of cerebral venous and sinus thrombosis. (orig.)

  18. Application of Computational Physics: Blood Vessel Constrictions and Medical Infuses

    CERN Document Server

    Suprijadi,; Subekti, Petrus; Viridi, Sparisoma

    2013-01-01

    Application of computation in many fields are growing fast in last two decades. Increasing on computation performance helps researchers to understand natural phenomena in many fields of science and technology including in life sciences. Computational fluid dynamic is one of numerical methods which is very popular used to describe those phenomena. In this paper we propose moving particle semi-implicit (MPS) and molecular dynamics (MD) to describe different phenomena in blood vessel. The effect of increasing the blood pressure on vessel wall will be calculate using MD methods, while the two fluid blending dynamics will be discussed using MPS. Result from the first phenomenon shows that around 80% of constriction on blood vessel make blood vessel increase and will start to leak on vessel wall, while from the second phenomenon the result shows the visualization of two fluids mixture (drugs and blood) influenced by ratio of drugs debit to blood debit. Keywords: molecular dynamic, blood vessel, fluid dynamic, movin...

  19. 77 FR 16039 - Abbott Laboratories et al.; Withdrawal of Approval of 35 New Drug Applications and 64 Abbreviated...

    Science.gov (United States)

    2012-03-19

    ... Drug Applications and 64 Abbreviated New Drug Applications AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) is withdrawing approval of 35 new drug applications (NDAs) and 64 abbreviated new drug applications (ANDAs) from multiple applicants. The holders...

  20. Harmful aquatic organisms in ballast water: application for basic approval of the VARUNA ballast water treatment system

    NARCIS (Netherlands)

    Jak, R.G.; Jongbloed, R.H.; Sneekes, A.C.; Kaag, N.H.B.M.

    2014-01-01

    This document contains the non-confidential information related to the application for Basic Approval of the VARUNA Ballast Water Treatment System submitted in accordance with the Procedure for approval of ballast water management systems that make use of Active Substances (G9) adopted by resolution

  1. 78 FR 33426 - Eli Lilly and Co.; Withdrawal of Approval of a New Drug Application for ORAFLEX

    Science.gov (United States)

    2013-06-04

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Eli Lilly and Co.; Withdrawal of Approval of a New Drug... Administration (FDA) is withdrawing approval of a new drug application (NDA) for ORAFLEX (benoxaprofen)...

  2. Pharmacogenomic information in FDA-approved drug labels: Application to pediatric patients.

    Science.gov (United States)

    Green, D J; Mummaneni, P; Kim, I W; Oh, J M; Pacanowski, M; Burckart, G J

    2016-06-01

    Pharmacogenomic (PGx) information is increasingly being incorporated into US Food and Drug Administration-approved drug labels. We reviewed the data source (adults vs. pediatrics) of PGx information in approved drug labels and assessed the suitability of applying adult-derived PGx information and related prescribing recommendations to the care of pediatric patients. We identified 65 drugs with labels containing PGx information and that have also been evaluated in children and found that in the majority of cases (56/65, 86%), the PGx information described was derived from adult studies. The application of PGx information from adults to pediatrics was deemed suitable for 71.4% (n = 40) of the drugs and unclear for 28.6% (n = 16). An ontogeny effect, limited or conflicting data regarding ontogeny of the genetic biomarker, or a difference in the pathophysiology or progression of the adult vs. pediatric disease were the primary reasons for deeming direct application from adults to pediatrics unclear.

  3. Android Blood Donor Life Saving Application in Cloud Computing

    Directory of Open Access Journals (Sweden)

    T.Hilda Jenipha

    2016-07-01

    Full Text Available Emergency situations, such as accidents, create an immediate, critical need for specific blood type. In addition to emergency requirements, advances in medicine have increased the need for blood in many on-going treatments and elective surgeries. Despite increasing requirements for blood, only about 5% of the Indian population donates blood. In this paper we propose a new and efficient way to overcome such scenarios with our project. We have to create a new idea, just touch the button. Donor will be prompted to enter an individual's details, like name, phone number, and blood type. After that your contact details will appear in alphabetical order on the screen; the urgent time of a blood requirement, you can quickly check for contacts matching a particular or related blood group and reach out to them via Phone Call/SMS through the Blood donor App. Blood Donor App provides list of donors in your city/area. Use this app in case of emergency. A large number of blood donors are attracted using an Android application. Cloud- based services can prove important in emergency blood delivery since they can enable central and immediate access to donors' data and location from anywhere. Since almost everyone carries a mobile phone with him, it ensures instant location tracking and communication. The location-based app, operational on android platform, will help users easily find donors of matching blood groups in their location and access their mobile numbers for instant help. Only a registered person, with willingness to donate blood, will be able to access the service.

  4. 76 FR 64951 - Apothecon et al.; Withdrawal of Approval of 103 New Drug Applications and 35 Abbreviated New Drug...

    Science.gov (United States)

    2011-10-19

    ... HUMAN SERVICES Food and Drug Administration Apothecon et al.; Withdrawal of Approval of 103 New Drug Applications and 35 Abbreviated New Drug Applications; Correction AGENCY: Food and Drug Administration, HHS... new drug applications (NDAs) and 35 abbreviated new drug applications (ANDAs) from multiple...

  5. 78 FR 48177 - Purdue Pharma L.P.; Withdrawal of Approval of a New Drug Application for Oxycontin

    Science.gov (United States)

    2013-08-07

    .... FDA-2013-N-0883] Purdue Pharma L.P.; Withdrawal of Approval of a New Drug Application for Oxycontin...) Extended-Release Tablets, held by Purdue Pharma L.P. (Purdue), One Stamford Forum, Stamford, CT...

  6. 77 FR 31828 - Notice of Request for Revision of a Currently Approved Collection Application for Plant Variety...

    Science.gov (United States)

    2012-05-30

    ... Information or Comments: Contact Bernadette Thomas, Information Technology Specialist, Plant Variety Protection Office (PVPO), Science and Technology, AMS, Room 401, National Agricultural Library (NAL), 10301... and revision to the currently approved information collection ``Application for ] Plant...

  7. 75 FR 36060 - Notice of Request for Extension of Approval of an Information Collection; Blood and Tissue...

    Science.gov (United States)

    2010-06-24

    ... Collection; Blood and Tissue Collection at Slaughtering and Rendering Establishments AGENCY: Animal and Plant... collection associated with regulations for blood and tissue collection at slaughtering and rendering... FURTHER INFORMATION CONTACT: For information on regulations for blood and tissue collection...

  8. 76 FR 59144 - Novartis Pharmaceuticals Corp. et al.; Withdrawal of Approval of 27 New Drug Applications and 58...

    Science.gov (United States)

    2011-09-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Novartis Pharmaceuticals Corp. et al.; Withdrawal of Approval of 27 New Drug Applications and 58 Abbreviated New Drug Applications; Correction AGENCY: Food...

  9. 21 CFR 515.25 - Revocation of order refusing to approve a medicated feed mill license application or suspending...

    Science.gov (United States)

    2010-04-01

    ... medicated feed mill license application or suspending or revoking a license. 515.25 Section 515.25 Food and..., FEEDS, AND RELATED PRODUCTS MEDICATED FEED MILL LICENSE Administrative Actions on Licenses § 515.25 Revocation of order refusing to approve a medicated feed mill license application or suspending or revoking...

  10. 21 CFR 314.107 - Effective date of approval of a 505(b)(2) application or abbreviated new drug application under...

    Science.gov (United States)

    2010-04-01

    ... introduction into interstate commerce when approval of the application or abbreviated application for the drug... for 5 years of exclusive marketing under § 314.108(b)(2) and the patent owner or its representative or... application first commences commercial marketing of its drug product; or (ii) The date of a decision of...

  11. 77 FR 25192 - Wild Bird Conservation Act; Receipt of Application for Approval of a Cooperative Breeding Program

    Science.gov (United States)

    2012-04-27

    ... Cooperative Breeding Program AGENCY: Fish and Wildlife Service, Interior. ACTION: Notice of receipt of... listed birds for scientific research, zoological breeding or display programs, or personal pet purposes, when the applicant meets certain criteria. We also may approve cooperative breeding programs of...

  12. 78 FR 2451 - Grand Gulf ESP Site; Consideration of Approval of Application Regarding Proposed Creation of a...

    Science.gov (United States)

    2013-01-11

    ... COMMISSION Grand Gulf ESP Site; Consideration of Approval of Application Regarding Proposed Creation of a... (ESP) ESP-002, for the ] Grand Gulf ESP Site, currently held by System Energy Resources, Inc. (SERI). The direct transfer of the Grand Gulf ESP would be to a new limited liability company, System...

  13. 78 FR 76327 - Notice of Approval of South Carolina's Application for Avoidance of 2013 Credit Reduction Under...

    Science.gov (United States)

    2013-12-17

    ... Employment and Training Administration Notice of Approval of South Carolina's Application for Avoidance of... avoidance of the 2013 credit reduction under this section. It has been determined that South Carolina met all of the criteria of section 3302(g) and thus qualifies for credit reduction avoidance....

  14. 76 FR 33756 - Notice of Approval of the Primacy Application for National Primary Drinking Water Regulations for...

    Science.gov (United States)

    2011-06-09

    ... AGENCY Notice of Approval of the Primacy Application for National Primary Drinking Water Regulations for... following offices: (1) Missouri Department of Natural Resources, Public Drinking Water Branch, 1101... Pesticides Division, Drinking Water Management Branch, 901 North 5th Street, Kansas City, Kansas 66101....

  15. 21 CFR 814.9 - Confidentiality of data and information in a premarket approval application (PMA) file.

    Science.gov (United States)

    2010-04-01

    ... secret or confidential commercial or financial information under § 20.61. (3) Any adverse reaction report... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Confidentiality of data and information in a premarket approval application (PMA) file. 814.9 Section 814.9 Food and Drugs FOOD AND DRUG...

  16. 28 CFR 58.17 - Procedures for denying an application or removing an agency from the approved list, and the...

    Science.gov (United States)

    2010-07-01

    ... credit counseling agency. (b) No administrative review will be granted to any applicant that submitted an... supplemented by, an interim directive, which may immediately remove an agency from the approved list. Such an interim directive may be issued if one or more of the following are specifically found: (1) The agency...

  17. 75 FR 35044 - Notice of Approval of a Supplemental New Animal Drug Application; Penicillin G Procaine Suspension

    Science.gov (United States)

    2010-06-21

    ... new animal drug application (NADA) filed by Norbrook Laboratories, Ltd. The supplemental NADA provides... 6JP, Northern Ireland, filed a supplement to NADA 065-010 for use of NOROCILLIN (penicillin G procaine... supplemental NADA is approved as of April 23, 2010. In accordance with the freedom of information provisions...

  18. Increased abundance of ADAM9 transcripts in the blood is associated with tissue damage [version 2; referees: 2 approved, 1 approved with reservations

    Directory of Open Access Journals (Sweden)

    Darawan Rinchai

    2016-10-01

    Full Text Available Background: Members of the ADAM (a disintegrin and metalloprotease domain family have emerged as critical regulators of cell-cell signaling during development and homeostasis. ADAM9 is consistently overexpressed in various human cancers, and has been shown to play an important role in tumorigenesis. However, little is known about the involvement of ADAM9 during immune-mediated processes. Results: Mining of an extensive compendium of transcriptomic datasets identified important gaps in knowledge regarding the possible role of ADAM9 in immunological homeostasis and inflammation: 1 The abundance of ADAM9 transcripts in the blood was increased in patients with acute infection but, 2 changed very little after in vitro exposure to a wide range of pathogen-associated molecular patterns (PAMPs. 3 Furthermore it was found to increase significantly in subjects as a result of tissue injury or tissue remodeling, in absence of infectious processes. Conclusions: Our findings indicate that ADAM9 may constitute a valuable biomarker for the assessment of tissue damage, especially in clinical situations where other inflammatory markers are confounded by infectious processes.

  19. Department of Health application for approval of construction SP-100 Ground Engineering System Test Site

    Energy Technology Data Exchange (ETDEWEB)

    1990-04-01

    The following Application For Approval of Construction is being submitted by the US Department of Energy-Richland Operations Office, for the SP-100 Ground Engineering System Test Site, which will provide a new source of radioactive emissions to the atmosphere. The US Department of Energy, the National Aeronautics and Space Administration, and the US Department of Defense have entered into an agreement to jointly develop space nuclear reactor power system technology. A ground test of a reactor is necessary to demonstrate technology readiness of this major subsystem before proceeding with the flight system development and demonstration. It is proposed that the SP-100 test reactor be tested in the existing decommissioned Plutonium Recycle Test Reactor containment building (309 Building). The reactor will be operated for at least three months and up to 2 yr. Following the test, the 309 Building will be decontaminated for potential use in other programs. It is projected this new source of emissions will contribute approximately 0.05 mrem/yr dose to the maximally exposed offsite individual. This application is being submitted in response to those projected emissions that would provide the described offsite dose. 28 refs., 9 figs., 7 tabs.

  20. Nocturnal Hypertension and Attenuated Nocturnal Blood Pressure Dipping is Common in Pediatric Lupus [version 2; referees: 2 approved, 1 approved with reservations

    Directory of Open Access Journals (Sweden)

    J. Fallon Campbell

    2015-11-01

    Full Text Available Hypertension is an important manifestation of systemic lupus erythematosus (SLE but reports of prevalence vary between 20-70% in published reports of adult and pediatric patients. For both children and adults with SLE, the clinical diagnosis and management of hypertension has traditionally been based on guidelines developed for the general population. In clinical trials, the criteria used for defining participants with hypertension are mostly undefined. As a first step towards formally assessing the blood pressure (BP patterns of children diagnosed with SLE, 24-hr ambulatory BP monitoring data was analyzed on clinic patients who presented with prehypertension or stage I hypertension. In this pediatric SLE cohort (n=10, 20% met daytime criteria for a diagnosis of hypertension. Patterns of BP elevation varied widely with white coat, masked, isolated systolic, and diastolic nocturnal hypertension all identified. Nocturnal hypertension was detected in 60% and attenuated nocturnal BP dipping in 90% of both hypertensive and normotensive SLE patients. In SLE patients, the median nighttime systolic and diastolic loads were 25% and 15.5% compared with median daily loads of 12.5% and 11.5%. Daytime and nighttime systolic and diastolic BP load and nocturnal dipping was compared to a control population consisting of 85 non-SLE patients under 21 years old with prehypertension or stage 1 hypertension presenting to hypertension clinic. Median systolic BP dipped 5.3 mmHg in SLE patients compared to 11.9 mmHg in non-lupus (p-value = 0.001. Median diastolic BP dipped 12.9 mmHg versus 18.5 mmHg in non-lupus (p-value = 0.003. Patterns of BP dysregulation in pediatric SLE merit further exploration. Children with or without SLE displaying prehypertensive or stage 1 casual BP measurements had similar rates of hypertension by ambulatory BP monitoring. However, regardless of BP diagnosis, and independent of kidney involvement, there was an increased proportion with

  1. 7 CFR 1436.9 - Loan amount and loan application approvals.

    Science.gov (United States)

    2010-01-01

    ... fees, except for filing fees; environmental and historic review fees including archaeological study... wiring; electrical motors; off-farm paid labor; on-farm site preparation and construction equipment costs... facility loans, the agency approval officials may only approve loans, subject to available funds....

  2. Application of CART Algorithm in Blood Donors Classification

    Directory of Open Access Journals (Sweden)

    T. Santhanam

    2010-01-01

    Full Text Available Problem statement: This study used data mining modeling techniques to examine the blood donor classification. The availability of blood in blood banks is a critical and important aspect in a healthcare system. Blood banks (in the developing countries context are typically based on a healthy person voluntarily donating blood and is used for transfusions or made into medications. The ability to identify regular blood donors will enable blood banks and voluntary organizations to plan systematically for organizing blood donation camps in an effective manner. Approach: Identify the blood donation behavior using the classification algorithms of data mining. The analysis had been carried out using a standard blood transfusion dataset and using the CART decision tree algorithm implemented in Weka. Results: Numerical experimental results on the UCI ML blood transfusion data with the enhancements helped to identify donor classification. Conclusion: The CART derived model along with the extended definition for identifying regular voluntary donors provided a good classification accuracy based model.

  3. Blood

    Science.gov (United States)

    ... Also, blood is either Rh-positive or Rh-negative. So if you have type A blood, it's either A positive or A negative. Which type you are is important if you need a blood transfusion. And your Rh factor could be important ...

  4. 2014 CRL Blood Pressure Study of Life Insurance Applicants.

    Science.gov (United States)

    Fulks, Michael; Dolan, Vera F; Stout, Robert L

    2015-01-01

    Objective .- Define the relative mortality risk by systolic (SBP) and diastolic blood pressure (DBP) in a relatively healthy cohort split by age and sex with adjustment for smoking status, other findings and admitted heart disease history. Method .- Blood pressure (BP in mm Hg), build, laboratory studies and limited medical history are collected when people apply for individual life insurance. Information on 2,472,706 applicants tested by Clinical Reference Laboratory from 1993 to 2007 was utilized with follow-up for vital status using the September 2011 Social Security Death Master File identifying 31,033 deaths. Data was analyzed by SBP and DBP split by age and sex accounting for smoking and for BMI, urine protein/creatinine ratio and history of heart disease in a Cox multivariate survival analysis. Separate analysis by admitted hypertension history was also conducted. Results are presented by SBP and DBP for 4 age-sex groups with and without added covariates beyond age and smoking status. Results .- Relative mortality progressively increased by SBP level from the 90 to 119 band (down to 80 in younger women) upward with little additional impact by DBP. Addition of covariates beyond age and smoking resulted in a 5% to 10% reduction in relative risk. Although high DBP had limited impact, a pulse pressure/SBP ratio >½ identified 1% of applicants at high mortality risk, with little difference in risk for ratios ≤½. Hypertension history with current BP control was associated with a 10% to 25% increase in relative mortality risk as compared to those with similar BP but no such history. Conclusion .- Increasing SBP is closely associated with increasing relative mortality, starting from the lowest SBP. Increasing DBP has little additional impact, but a pulse pressure/SBP ratio >½ is a potent marker of increased risk as well. Accounting for build and other laboratory findings reduces risk modestly. A history of hypertension with current control increases risk.

  5. 78 FR 52536 - Withdrawal of Approval of New Animal Drug Applications; Diethylcarbamazine; Nicarbazin...

    Science.gov (United States)

    2013-08-23

    ...; Diethylcarbamazine; Nicarbazin; Penicillin; Roxarsone AGENCY: Food and Drug Administration, HHS. ACTION: Notice... withdraw approval of NADA 098-371 for use of nicarbazin, penicillin, and roxarsone in 3-way,...

  6. The effect size, study design, and development experience in commercially sponsored studies for new drug applications in approved drugs

    OpenAIRE

    Fukunaga, Satoshi; Kusama, Makiko; Ono, Shunsuke

    2014-01-01

    Pharmaceutical companies incorporate different features into the trials for new drug applications (NDAs) to render them efficient, making use of their experience. The objective of this analysis was to examine the associations between outcome and features related to study design and clinical development experience in commercially sponsored clinical trials. We collected data of phase 2 and phase 3 trials of all the drugs that obtained approval for depression, schizophrenia, asthma, hypertension...

  7. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... infection) back to top How are Blood Clots in Pregnant Women Treated? Typically, blood clots are treated ... history of blood clots or blood clotting disorders in your family. Remain active, with your doctor's approval. ...

  8. Nanodiamonds for Medical Applications: Interaction with Blood in Vitro and in Vivo.

    Science.gov (United States)

    Tsai, Lin-Wei; Lin, Yu-Chung; Perevedentseva, Elena; Lugovtsov, Andrei; Priezzhev, Alexander; Cheng, Chia-Liang

    2016-07-12

    Nanodiamonds (ND) have emerged to be a widely-discussed nanomaterial for their applications in biological studies and for medical diagnostics and treatment. The potentials have been successfully demonstrated in cellular and tissue models in vitro. For medical applications, further in vivo studies on various applications become important. One of the most challenging possibilities of ND biomedical application is controllable drug delivery and tracing. That usually assumes ND interaction with the blood system. In this work, we study ND interaction with rat blood and analyze how the ND surface modification and coating can optimize the ND interaction with the blood. It was found that adsorption of a low concentration of ND does not affect the oxygenation state of red blood cells (RBC). The obtained in vivo results are compared to the results of in vitro studies of nanodiamond interaction with rat and human blood and blood components, such as red blood cells and blood plasma. An in vivo animal model shows ND injected in blood attach to the RBC membrane and circulate with blood for more than 30 min; and ND do not stimulate an immune response by measurement of proinflammatory cytokine TNF-α with ND injected into mice via the caudal vein. The results further confirm nanodiamonds' safety in organisms, as well as the possibility of their application without complicating the blood's physiological conditions.

  9. APPLICATION OF THE THEORY OF INTERACTING CONTINUA TO BLOOD FLOW

    Energy Technology Data Exchange (ETDEWEB)

    Massoudi, Mehrdad; Kim, Jeongho; Hund, Samuel J.; Antaki, James F.

    2011-01-01

    Micro-scale investigations of the flow and deformation of blood and its formed elements have been studied for many years. Early in vitro investigations in the rotational viscometers or small glass tubes revealed important rheological properties such as the reduced blood apparent viscosity, Fahraeus effect and Fahraeus-Lindqvist effect [1], exhibiting the nonhomogeneous property of blood in microcirculation. We have applied Mixture Theory, also known as Theory of Interacting Continua, to study and model this property of blood [2, 3]. This approach holds great promise for predicting the trafficking of RBCs in micro-scale flows (such as the depletion layer near the wall), and other unique hemorheological phenomena relevant to blood trauma. The blood is assumed to be composed of an RBC component modeled as a nonlinear fluid, suspended in plasma, modeled as a linearly viscous fluid.

  10. Nanodiamonds for Medical Applications: Interaction with Blood in Vitro and in Vivo

    Directory of Open Access Journals (Sweden)

    Lin-Wei Tsai

    2016-07-01

    Full Text Available Nanodiamonds (ND have emerged to be a widely-discussed nanomaterial for their applications in biological studies and for medical diagnostics and treatment. The potentials have been successfully demonstrated in cellular and tissue models in vitro. For medical applications, further in vivo studies on various applications become important. One of the most challenging possibilities of ND biomedical application is controllable drug delivery and tracing. That usually assumes ND interaction with the blood system. In this work, we study ND interaction with rat blood and analyze how the ND surface modification and coating can optimize the ND interaction with the blood. It was found that adsorption of a low concentration of ND does not affect the oxygenation state of red blood cells (RBC. The obtained in vivo results are compared to the results of in vitro studies of nanodiamond interaction with rat and human blood and blood components, such as red blood cells and blood plasma. An in vivo animal model shows ND injected in blood attach to the RBC membrane and circulate with blood for more than 30 min; and ND do not stimulate an immune response by measurement of proinflammatory cytokine TNF-α with ND injected into mice via the caudal vein. The results further confirm nanodiamonds’ safety in organisms, as well as the possibility of their application without complicating the blood’s physiological conditions.

  11. 76 FR 72955 - Wyeth Pharmaceuticals, Inc.; Withdrawal of Approval of a New Drug Application for MYLOTARG

    Science.gov (United States)

    2011-11-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Wyeth Pharmaceuticals, Inc.; Withdrawal of Approval of a New... (gemtuzumab ozogamicin) for Injection, held by Wyeth Pharmaceuticals, Inc. (Wyeth), 500 Arcola...

  12. FDA Approvals of Brand-Name Prescription Drugs in 2015.

    Science.gov (United States)

    2016-03-01

    The drugs included in this review were approved by the US Food and Drug Administration (FDA) in 2015 and are grouped into the following categories: New Pharmaceuticals: New Molecular Entities and New Biologic License ApplicationsNew Combinations and New IndicationsNew Dosage Forms and New FormulationsNew Biosimilars, Vaccines, Viral Therapies, and Blood Products.

  13. Application of the forensic Luminol for blood in infection control.

    Science.gov (United States)

    Bergervoet, P W M; van Riessen, N; Sebens, F W; van der Zwet, W C

    2008-04-01

    Transmission of hepatitis C virus occurs frequently in haemodialysis units. A possible route of transmission is indirectly via the hospital environment although this has never been recorded. We investigated the haemodialysis unit in Deventer Hospital, Deventer, The Netherlands, with the forensic Luminol test. With this test, invisible traces of blood can be visualised based on the principle of biochemiluminescence. We demonstrated extensive contamination of the environment with traces of blood. The aim of this article is to introduce this method to infection control professionals, so it can be used to monitor cleaning and disinfection procedures, and alert healthcare workers to the possibility of contamination of the hospital environment with blood.

  14. 12 CFR 516.290 - What will happen if OTS does not approve or disapprove my application within two calendar years...

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What will happen if OTS does not approve or disapprove my application within two calendar years after the filing date? 516.290 Section 516.290 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES OTS Review Standard Treatment § 516.290...

  15. Current molecular blood group technology:availability and practical applications

    Institute of Scientific and Technical Information of China (English)

    Willy A.Flegel

    2010-01-01

    @@ Almost all clinically important RBC antigens are defined at the molecular level.The expression of protein-and sugar-based antigens on the RBC surface can be predicted by determining the blood group gene variants(alleles).Most of the time,a single nucleotide polymorphism(sNP)distinguishes the allele,which determines an antigen and hence allows predicting the antigen.PCR with sequence specific priming(PCR-SSP)followed by gel electrophoresis was the original technique widely applied for blood group genotyping.Realtime PCR obviated the need for gels.

  16. 77 FR 8865 - Public Water System Supervision Program Approval for the State of Illinois; Tentative Approval

    Science.gov (United States)

    2012-02-15

    ... From the Federal Register Online via the Government Publishing Office ENVIRONMENTAL PROTECTION AGENCY Public Water System Supervision Program Approval for the State of Illinois; Tentative Approval... State of Illinois submitted a primacy application for its approved Public Water System...

  17. 14 CFR 303.04 - General rules governing application content, procedure and conditions of approval.

    Science.gov (United States)

    2010-01-01

    ... applicants shall file an affidavit executed by the individual responsible for the search explaining why they... is filed with the Documentary Services Division. (j) The applicant shall, if requested,...

  18. [Peculiarities of application of a cell saver apparatus in neonathal cardiosurgery using artificial blood circulation].

    Science.gov (United States)

    Kuz'menko, S O; Chasovs'kyĭ, K S

    2015-01-01

    Elaborated and introduced into the practice method of the blood preservation, while correction of complex inborn heart failures (IHF) in a newborn babies, was proposed. It assumes application of system for intraoperative reinfusion of own erythrocytes with processing of residual perfusate and their reinfusion in a postperfusion period. Impact of the blood preservation on volume of a donor's blood components, hematological indices and methods of application of washed erythrocytes while correction of complex IHF in a newborn babies were presented. The method was applied in 47 newborn babies, to whom an arterial switch was performed for the main vessels transposition.

  19. Applicability of available methods for incidence estimation among blood donors

    Institute of Scientific and Technical Information of China (English)

    Shtmian Zou; Edward P.Notari IV; Roger Y.Dodd

    2010-01-01

    @@ Abstract Incidence rates of major transfusion transmissible viral infections have been estimated threugh widely used sereconversion approaches and recently developed methods.A quality database for blood donors and donations with the capacity to track donation history of each donor is the basis for incidence estimation and many other epidemiological studies.Depending on available data,difierent ways have been used to determine incidence rates based on conversion from uninfected to infected status among repeat donors.

  20. 21 CFR 1312.31 - Schedule I: Application for prior written approval.

    Science.gov (United States)

    2010-04-01

    ... IMPORTATION AND EXPORTATION OF CONTROLLED SUBSTANCES Transshipment and In-Transit Shipment of Controlled... documents or written statements of fact relevant to the application as he deems necessary to determine... applicant of an opportunity to present such documents or facts for consideration by the Administrator...

  1. Ultrasonic monitoring of droplets' evaporation: Application to human whole blood.

    Science.gov (United States)

    Laux, D; Ferrandis, J Y; Brutin, D

    2016-09-01

    During a colloidal droplet evaporation, a sol-gel transition can be observed and is described by the desiccation time τD and the gelation time τG. These characteristic times, which can be linked to viscoelastic properties of the droplet and to its composition, are classically rated by analysis of mass droplet evolution during evaporation. Even if monitoring mass evolution versus time seems straightforward, this approach is very sensitive to environmental conditions (vibrations, air flow…) as mass has to be evaluated very accurately using ultra-sensitive weighing scales. In this study we investigated the potentialities of ultrasonic shear reflectometry to assess τD and τG in a simple and reliable manner. In order to validate this approach, our study has focused on blood droplets evaporation on which a great deal of work has recently been published. Desiccation and gelation times measured with shear ultrasonic reflectometry have been perfectly correlated to values obtained from mass versus time analysis. This ultrasonic method which is not very sensitive to environmental perturbations is therefore very interesting to monitor the drying of blood droplets in a simple manner and is more generally suitable for complex fluid droplets evaporation investigation.

  2. Application of atomic force microscopy in blood research

    Institute of Scientific and Technical Information of China (English)

    Xiao-Long Ji; Ya-Min Ma; Tong Yin; Ming-Shi Shen; Xin Xu; Wei Guan

    2005-01-01

    AIM: To find suitable solutions having lesser granules and keeping erythrocytes in normal shapes under atomic force microscopy (AFM).METHODS: Eight kinds of solutions, 1% formaldehyde,PBS buffer (pH7.2), citrate buffer (pH6,0), 0.9% NaCl,5% dextrose, TAE, 1640 medium and 5% EDTA-K2, were selected from commonly used laboratory solutions, and venous blood from a healthy human volunteer was drawn and anticoagulated with EDTA-K2. Before scanned by AFM (NanoScopeⅢa SPM, Digital Instruments, Santa Barbara,CA), a kind of intermixture was deposited on freshly cleaved mica and then dried in the constant temperature cabinet (37 ℃).RESULTS: One percent formaldehyde, citrate buffer, 5%dextrose, TAE, were found to keep human erythrocytes in normal shape with few particles. Processed by these solutions, fine structures of human erythrocyte membrane were obtained.CONCLUSION: One percent formaldehyde, citrate buffer,5% dextrose and TAE may be applied to disposeerythrocytes in AFM. The results may offer meaningful data for clinical diagnosis of blood by AFM.

  3. 77 FR 60442 - Withdrawal of Approval of New Animal Drug Applications; Butorphanol; Doxapram; Triamcinolone...

    Science.gov (United States)

    2012-10-03

    ...; Butorphanol; Doxapram; Triamcinolone; Tylosin AGENCY: Food and Drug Administration, HHS. ACTION: Notice.../ANADA No. Trade name (drug) Applicant 100-556 Vigorena Feeds Hy-Ty Springfield Milling Premix...

  4. The application of silicon sol-gel technology to forensic blood substitute development: Mimicking aspects of whole human blood rheology.

    Science.gov (United States)

    Stotesbury, Theresa; Illes, Mike; Wilson, Paul; Vreugdenhil, Andrew J

    2017-01-01

    Solution-gelation chemistry has promising applications in forensic synthetic blood substitute development. This research offers a silicon-based sol-gel approach to creating stable materials that share similar rheological properties to that of whole human blood samples. Room temperature, high water content, silicon sol-gels were created using the organosilane precursors 3-glycidoxypropyltrimethoxysilane and tetraethylorthosilicate along with various concentrations of filler and pigment. Shear-thinning non-Newtonian properties were observed within most formulations of the presented materials. The effects of colloidal concentration, temperature, age and filler addition on the viscosity of the sol-gels were investigated. SEM-EDS analysis was used to identify the behavior of the fillers within the film and support their inclusion for basic bloodstain pattern simulation. A final proposed candidate sol-gel was assessed using a previously reported passive drip simulation test on a hard, dry surface and passed. This works represents encouraging development in providing safe material alternatives to using whole human blood for forensic training and research.

  5. Platelet proteomics and its advanced application for research of blood stasis syndrome and activated blood circulation herbs of Chinese medicine.

    Science.gov (United States)

    Liu, Yue; Yin, Huijun; Chen, Keji

    2013-11-01

    The development of novel and efficient antiplatelet agents that have few adverse effects and methods that improve antiplatelet resistance has long been the focus of international research on the prevention and treatment of cardiovascular and cerebrovascular diseases. Recent advances in platelet proteomics have provided a technology platform for high-quality research of platelet pathophysiology and the development of new antiplatelet drugs. The study of blood stasis syndrome (BSS) and activated blood circulation of traditional Chinese medicine (TCM) is one of the most active fields where the integration of TCM and western medicine in China has been successful. Activated blood circulation herbs (ABC herbs) of Chinese medicine are often used in the treatment of BSS. Most ABC herbs have antiplatelet and anti-atherosclerosis activity, but knowledge about their targets is lacking. Coronary heart disease (CHD), BSS, and platelet activation are closely related. By screening and identifying activated platelet proteins that are differentially expressed in BSS of CHD, platelet proteomics has helped researchers interpret the antiplatelet mechanism of action of ABC herbs and provided many potential biomarkers for BSS that could be used to evaluate the clinical curative effect of new antiplatelet drugs. In this article the progress of platelet proteomics and its advanced application for research of BSS and ABC herbs of Chinese medicine are reviewed.

  6. CFD Application in Implantable Rotary Blood Pump Design and Validation

    Institute of Scientific and Technical Information of China (English)

    YI Qian

    2004-01-01

    Implantable rotary blood pump (IRBP) has been promoted to the stage of clinical trial. This paper introduces a unique IRBP without a shaft. Instead of using thrombogenic pivots or power-drawing magnetic suspension, impeller is supported hydrodynamically when rotating, by lubrication flows in the thin spaces between itself and the pump body. To this end, the flow is very difficult to be measured using usual laboratory equipments. Therefore, computational fluid dynamics (CFD) has been applied as an important tool in the IRBP design and its validation procedure. Several CFD results such as pump performance improvement, unsteady hydraulic dynamic analysis, biocapability prediction, validation and verification (V&V), and flow visualization have been performed.

  7. CFD Application in Implantable Rotary Blood Pump Design and Validation

    Institute of Scientific and Technical Information of China (English)

    YIQian

    2004-01-01

    Implantable rotary blood pump (IRBP) has been promoted to the stage of clinical trial. This paper introduces a unique IRBP without a.shaft. Instead of using thrombogenic pivots or power-drawing magnetic suspension, impeller is supported hydrodynamically when rotating, by lubrication flows in the thin spaces between itself and the pump body. To this end, the flow is very difficult to be measured using usual laboratory equipments. Therefore, computational fluid dynamics (CFD) has been applied as an important tool in the IRBP design and its validation procedure. Several CFD results such as pump performance improvement, unsteady hydraulic dynamic analysis, biocapability prediction, validation and verification (V&V), and flow visualization have been performed.

  8. Laser optical method of visualizing cutaneous blood vessels and its applications in biometry and photomedicine

    Science.gov (United States)

    Asimov, M. M.; Asimov, R. M.; Rubinov, A. N.

    2011-05-01

    We propose and examine a new approach to visualizing a local network of cutaneous blood vessels using laser optical methods for applications in biometry and photomedicine. Various optical schemes of the formation of biometrical information on the architecture of blood vessels of skin tissue are analyzed. We developed an optical model of the interaction of the laser radiation with the biological tissue and a mathematical algorithm of processing of measurement results. We show that, in medicine, the visualization of blood vessels makes it possible to calculate and determine regions of disturbance of blood microcirculation and to control tissue hypoxia, as well as to maintain the local concentration of oxygen at a level necessary for the normal cellular metabolism. We propose noninvasive optical methods for modern photomedicine and biometry for diagnostics and elimination of tissue hypoxia and for personality identification and verification via the pattern of cutaneous blood vessels.

  9. Effect of extraluminal ATP application on vascular tone and blood flow in skeletal muscle

    DEFF Research Database (Denmark)

    Nyberg, Michael Permin; Al-Khazraji, Baraa K; Mortensen, Stefan P;

    2013-01-01

    During skeletal muscle contractions, the concentration of ATP increases in muscle interstitial fluid as measured by microdialysis probes. This increase is associated with the magnitude of blood flow, suggesting that interstitial ATP may be important for contraction-induced vasodilation. However...... in interstitial ATP concentrations increases muscle blood flow, indicating that the contraction-induced increase in skeletal muscle interstitial [ATP] is important for exercise hyperemia. The vasodilator effect of ATP application is mediated by NO and prostanoid formation....

  10. Application of quantitative ethanol detector (QED) test kit to measure ethanol concentration in blood samples.

    Science.gov (United States)

    Biwasaka, H; Tokuta, T; Sasaki, Y; Niitsu, H; Kumagai, R; Aoki, Y

    2001-12-27

    In this paper, the applicability of the quantitative ethanol detector (QED) test kit for screening of ethanol concentrations in blood samples was investigated. The pretreatment of blood using the sulfosalicylic acid solution and the three-way stopcock followed by membrane filtration gave satisfactory results. The ethanol concentrations in whole blood samples (n=61) determined by QED correlated well with those determined by gas chromatography; the correlation coefficient indicated 0.990. Because a high correlation coefficient (0.928) was also confirmed in trial by investigators, QED test should be highly considered for ethanol screening in forensic praxis.

  11. [Applications and approved projectsof general program, young scientist fund and fund for less developedregion of national natural science funds in discipline of Chinese materia medica, NSFC in 2012].

    Science.gov (United States)

    Huang, Ming-Qing; Han, Li-Wei; Wu, Xiu-Hong; Bi, Ming-Gang; Shang, Hong-Cai; Liu, Yun-Fang; He, Wei-Ming; Li, Dan-Dan; Dong, Yan; Wang, Chang-En

    2013-01-01

    The applications accepted and approved by general program, young scientist fund and fund for less developed region of national natural science funds in the discipline of Chinese materia medica, NSFC in 2012 have been introduced. The research contents of the funded projects in the popular research areas have been summarized and the problems in the applications have been analyzed to give a reference to the scientists in the field of Chinese materia medica.

  12. Fish peripheral blood mononuclear cells preparation for future monitoring applications.

    Science.gov (United States)

    Pierrard, Marie-Aline; Roland, Kathleen; Kestemont, Patrick; Dieu, Marc; Raes, Martine; Silvestre, Frédéric

    2012-07-15

    Fish species possess many specific characteristics that support their use in ecotoxicology. Widely used in clinical research, peripheral blood mononuclear cells (PBMCs) can reasonably be exploited as relevant target cells in the assessment of environmental chemical toxicity. The current article focuses on the methods necessary to isolate, characterize, and culture fish PBMCs. These procedures were successfully applied on an endangered species, the European eel (Anguilla anguilla L.), and on an economically important and worldwide exported species, the Asian catfish (Pangasianodon hypophthalmus S.). Proteomic approaches can be useful to screen xenobiotic exposure at the protein expression level, giving the opportunity to develop early warning signals thanks to molecular signatures of toxicity. To date, a major limitation of proteomic analyses is that most protein expression profiles often reveal the same predominant and frequently differentially expressed families of proteins regardless of the experimental stressing conditions. The current study describes a methodology to get a postnuclear fraction of high quality isolated from fish PBMCs in order to perform subsequent subproteomic analyses. Applied on samples from eel, the subproteomic analysis (two-dimensional differential in-gel electrophoresis) allowed the identification by liquid chromatography-tandem mass spectrometry and searches in the full NCBInr (National Center for Biotechnology Information nonredundant) database of 66 proteins representing 36 different proteins validated through Peptide and Protein Prophet of Scaffold software.

  13. 78 FR 730 - State Program Requirements; Approval of Application To Administer Partial National Pollutant...

    Science.gov (United States)

    2013-01-04

    ... Elimination System program under the Clean Water Act. SUMMARY: On December 20, 2012, the Regional... pursuant to Section 402(b) of the Clean Water Act (CWA or ``the Act''). The AgPDES program will be..., Texas 75202. A copy of ODAFF's application is available online at the EPA Region 6 web page:...

  14. 78 FR 64423 - Children's Online Privacy Protection Rule Applications for Approval of Proposed Parental Consent...

    Science.gov (United States)

    2013-10-29

    ..., such as Social Security number, date of birth, driver's license number or other state identification... determinations on the applications. The federal government re- opened on October 17, 2013. In order to ensure... order to account for the time period in which the government was shut down. Accordingly, the...

  15. 78 FR 67985 - Supplemental Applications Proposing Labeling Changes for Approved Drugs and Biological Products

    Science.gov (United States)

    2013-11-13

    ... therapy. As a drug is used more widely or under diverse conditions, new information regarding the risks... applicant should submit a proposed narrative description of the proposed labeling change in the CBE-0 supplement for posting on the FDA Web page. This brief narrative description should include the...

  16. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... pregnancy: Be aware of risk factors. Know your family history. Make sure your doctor knows about any ... blood clots or blood clotting disorders in your family. Remain active, with your doctor's approval. Be aware ...

  17. Isolation of plasma from whole blood using planar microfilters for lab-on-a-chip applications.

    Science.gov (United States)

    Crowley, Timothy A; Pizziconi, Vincent

    2005-09-01

    Researchers are actively developing devices for the microanalysis of complex fluids, such as blood. These devices have the potential to revolutionize biological analysis in a manner parallel to the computer chip by providing very high throughput screening of complex samples and massively parallel bioanalytical capabilities. A necessary step performed in clinical chemistry is the isolation of plasma from whole blood, and effective sample preparation techniques are needed for the development of miniaturized clinical diagnostic devices. This study demonstrates the use of passive, operating entirely on capillary action, transverse-flow microfilter devices for the microfluidic isolation of plasma from whole blood. Using these planar microfilters, blood can be controllably fractionated with minimal cell lysis. A characterization of the device performance reveals that plasma filter flux is dependent upon the wall shear rate of blood in the filtration channel, and this result is consistent with macroscale blood filtration using microporous membranes. Also, an innovative microfluidic layout is demonstrated that extends device operation time via capillary action from seconds to minutes. Efficiency of these microfilters is approximately three times higher than the separation efficiencies predicted for microporous membranes under similar conditions. As such, the application of the microscale blood filtration designs used in this study may have broad implications in the design of lab-on-a-chip devices, as well as the field of separation science.

  18. Genomic variant annotation workflow for clinical applications [version 2; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Thomas Thurnherr

    2016-10-01

    Full Text Available Annotation and interpretation of DNA aberrations identified through next-generation sequencing is becoming an increasingly important task. Even more so in the context of data analysis pipelines for medical applications, where genomic aberrations are associated with phenotypic and clinical features. Here we describe a workflow to identify potential gene targets in aberrated genes or pathways and their corresponding drugs. To this end, we provide the R/Bioconductor package rDGIdb, an R wrapper to query the drug-gene interaction database (DGIdb. DGIdb accumulates drug-gene interaction data from 15 different resources and allows filtering on different levels. The rDGIdb package makes these resources and tools available to R users. Moreover, rDGIdb queries can be automated through incorporation of the rDGIdb package into NGS sequencing pipelines.

  19. Measurements of blood flow and blood concentration change using laser speckle in fiber illumination and its application to estimation of stress condition

    Science.gov (United States)

    Yokoi, Naomichi; Shinohara, Tomomi; Funamizu, Hideki; Kyoso, Masaki; Shimatani, Yuichi; Yuasa, Tomonori; Aizu, Yoshihisa

    2016-11-01

    Speckle imaging method is useful for monitoring of blood flow in living bodies. We have proposed so far the method for simultaneous imaging of blood flow and blood concentration change using laser speckle patterns at two wavelengths. However, our conventional measurement system has difficulty in adjusting the illuminating optical axis of two laser sources. Therefore, we introduce a novel arrangement using a coaxial fiber illumination in the detection of speckle patterns in two wavelengths. By this arrangement, the blood flow can be stably analyzed with a frame rate using an estimation parameter proposed by the authors based on the spatial contrast of speckle patterns. This parameter is useful for estimating an autonomic nervous function which reflects stress conditions caused by tension and excitement. In this study, we present measurements of the blood flow and blood concentration change in the fiber illumination, and its application to estimation of stress condition.

  20. State waste discharge permit application for the 200 Area Effluent Treatment Facility and the State-Approved Land Disposal Site

    Energy Technology Data Exchange (ETDEWEB)

    1993-08-01

    Application is being made for a permit pursuant to Chapter 173--216 of the Washington Administrative Code (WAC), to discharge treated waste water and cooling tower blowdown from the 200 Area Effluent Treatment Facility (ETF) to land at the State-Approved Land Disposal Site (SALDS). The ETF is located in the 200 East Area and the SALDS is located north of the 200 West Area. The ETF is an industrial waste water treatment plant that will initially receive waste water from the following two sources, both located in the 200 Area on the Hanford Site: (1) the Liquid Effluent Retention Facility (LERF) and (2) the 242-A Evaporator. The waste water discharged from these two facilities is process condensate (PC), a by-product of the concentration of waste from DSTs that is performed in the 242-A Evaporator. Because the ETF is designed as a flexible treatment system, other aqueous waste streams generated at the Hanford Site may be considered for treatment at the ETF. The origin of the waste currently contained in the DSTs is explained in Section 2.0. An overview of the concentration of these waste in the 242-A Evaporator is provided in Section 3.0. Section 4.0 describes the LERF, a storage facility for process condensate. Attachment A responds to Section B of the permit application and provides an overview of the processes that generated the wastes, storage of the wastes in double-shell tanks (DST), preliminary treatment in the 242-A Evaporator, and storage at the LERF. Attachment B addresses waste water treatment at the ETF (under construction) and the addition of cooling tower blowdown to the treated waste water prior to disposal at SALDS. Attachment C describes treated waste water disposal at the proposed SALDS.

  1. The effect size, study design, and development experience in commercially sponsored studies for new drug applications in approved drugs.

    Science.gov (United States)

    Fukunaga, Satoshi; Kusama, Makiko; Ono, Shunsuke

    2014-01-01

    Pharmaceutical companies incorporate different features into the trials for new drug applications (NDAs) to render them efficient, making use of their experience. The objective of this analysis was to examine the associations between outcome and features related to study design and clinical development experience in commercially sponsored clinical trials. We collected data of phase 2 and phase 3 trials of all the drugs that obtained approval for depression, schizophrenia, asthma, hypertension, and diabetes in Japan from 1970 to 2011. In total, 145 trials from 90 test drugs were eligible for our study. We calculated the effect size, the standard mean of differences between test drug and comparator therapeutic effects, as the objective variable for use in our analysis. A linear mixed effect model with nested and crossed random effects was used in the analysis including variety of therapeutic area, test drugs and clinical trials. The analysis showed that trial features including sample size, subjective endpoints and active comparator of the same mode of action were negatively associated with effect size. In addition, sponsors' domestic clinical development experience with similar drugs seemed to have a positive association, but prior development experience in foreign countries did not. The accumulation of skills and knowledge within sponsors, and accumulated experience in domestic professionals who implement clinical trials under study contracts with sponsors would be of great importance for yielding clear outcomes. This study provides additional evidence with respect to possible sizes and directions of the influence of study design features that must be considered when planning and implementing trials for new drug applications, and when retrospectively comparing outcomes from trials with different designs and environments.

  2. Prevention of significant deterioration application for approval to construct SP-100 Ground Engineering System Test Site

    Energy Technology Data Exchange (ETDEWEB)

    1990-04-01

    The following application is being submitted by the US Department of Energy, Richland Operations Office, P.O. Box 550, Richland, Washington 99352, pursuant to WAC 173-403-080, and in compliance with the Department of Ecology Guide to Processing a Prevention of Significant Deterioration (PSD) Permit'' for a new source of airborne radionuclide emissions at the Hanford Site in Washington State. The new source, the SP-100 Ground Engineering System (GES) Test Site, will be located in the 309 Building of the 300 Area. The US Department of Energy (DOE), the National Aeronautics and Space Administration (NASA), and the US Department of Defense (DOD) have entered into an agreement to jointly develop space nuclear reactor power system technology. The DOE has primary responsibility for developing and ground testing the nuclear subsystem. A ground test of a reactor is necessary to demonstrate technology readiness of this major subsystem before proceeding with the flight system development and demonstration. The SP-100 GES Test Site will provide a location for the operation and testing of a prototype space-based, liquid metal-cooled, fast flux nuclear reactor in an environment closely simulating the vacuum and temperature conditions of space operations. The purpose of the GES is to develop safe, compact, light-weight and durable space reactor power system technology. This technology will be used to provide electric power, in the range of tens to hundreds of kilowatts, for a variety of potential future civilian and military space missions requiring long-term, high-power level sources of energy. 20 refs., 8 figs., 7 tabs.

  3. APPLICATION OF MALARIA DETECTION OF DRAWING BLOOD CELLS USING MICROSCOPIC OpenCV

    Directory of Open Access Journals (Sweden)

    Antonius Herusutopo

    2011-10-01

    Full Text Available The goal of the research is to produce an application, which can detect malaria on patient through microscopic digital image of blood sample. The research methods are data collection, design analysis, testing and evaluation. The used application methods are image pre-processing, morphology and image segmentation using OpenCV. The expected result is a creation of application, which can be able to detect malaria on a microscopic digital image of patient blood sample. The conclusion is that the application can detect malaria from young trophozoites stadium and gametesocytes from the picture.Keywords: Detection; Malaria; Computer Vision; OpenCVINTRODUCTIONSystem technology of computer-based with artificial intelligence already can be used in medicine field, for example, to resolve the problems: detecting specific disease and its symptoms, analyzing the content of a sample, monitoring the condition of an organ, and others. Nevertheless, the medical field is very wide, so for detecting diseases problems, not yet much disease that detection can be done with a computer-based system. One example of the issues is well-known disease detection, which is malaria. Malaria is classified as a serious disease because it can cause death if it is not treated properly. Malaria has various types and can affect anyone anywhere. The symptoms of malaria is really common as it may appear in daily life, but cannot always indicate that a person infected with malaria. Indications, which can show that a person infected with malaria, are the clinical examination and blood tests.With the blood test, the treatment of malaria can be implemented correctly and precisely. It needs technology that can detect malaria correctly and precisely. The solution is the method of support vector machine that can detect malaria in humans by viewing image of appearance blood cells.METHODThe methods used in this research are data collection, analysis and design. The data collection includes

  4. A programmable and portable NMES device for drop foot correction and blood flow assist applications.

    Science.gov (United States)

    Breen, Paul P; Corley, Gavin J; O'Keeffe, Derek T; Conway, Richard; Olaighin, Gearóid

    2009-04-01

    The Duo-STIM, a new, programmable and portable neuromuscular stimulation system for drop foot correction and blood flow assist applications is presented. The system consists of a programmer unit and a portable, programmable stimulator unit. The portable stimulator features fully programmable, sensor-controlled, constant-voltage, dual-channel stimulation and accommodates a range of customized stimulation profiles. Trapezoidal and free-form adaptive stimulation intensity envelope algorithms are provided for drop foot correction applications, while time dependent and activity dependent algorithms are provided for blood flow assist applications. A variety of sensor types can be used with the portable unit, including force sensitive resistor-based foot switches and MEMS-based accelerometer and gyroscope devices. The paper provides a detailed description of the hardware and block-level system design for both units. The programming and operating procedures for the system are also presented. Finally, functional bench test results for the system are presented.

  5. Blood compatibility of a ferulic acid (FA)-eluting PHBHHx system for biodegradable magnesium stent application.

    Science.gov (United States)

    Zhang, Erlin; Shen, Feng

    2015-01-01

    Magnesium stent has shown potential application as a new biodegradable stent. However, the fast degradation of magnesium stent limited its clinic application. Recently, a biodegradable and drug-eluting coating system was designed to prevent magnesium from fast degradation by adding ferulic acid (FA) in poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by a physical method. In vitro study has demonstrated that the FA-eluting system exhibited strong promotion to the endothelialization, which might be a choice for the stent application. In this paper, the hemolysis rate, the plasma recalcification time (PRT), the plasma prothrombin time (PT) and the kinetic clotting time of the FA-eluting films were investigated and the platelet adhesion was observed in order to assess the blood compatibility of the FA-eluting PHBHHx films in comparison with PHBHHx film. The results have shown that the addition of FA had no influence on the hemolysis, but prolonged PRT, PT and the clotting time and reduced the platelet adhesion and activation, displaying that the FA-eluting PHBHHx exhibited better blood compatibility than PHBHHx. In addition, the effect of alkali treatment on the blood compatibility of FA-eluting PHBHHx was also studied. It was indicated that alkali treatment had no effect on the hemolysis and the coagulation time, but enhanced slightly the platelet adhesion. All these demonstrated that FA-eluting PHBHHx film had good blood compatibility and might be a candidate surface coating for the biodegradable magnesium stent.

  6. Roll-to-roll, shrink-induced superhydrophobic surfaces for antibacterial applications, enhanced point-of-care detection, and blood anticoagulation

    Science.gov (United States)

    Nokes, Jolie McLane

    Superhydrophobic (SH) surfaces are desirable because of their unique anti-wetting behavior. Fluid prefers to bead up (contact angle >150°) and roll off (contact angle hysteresis air pockets. Fluid only adheres to the peaks of the structures, causing minimal adhesion to the surface. Here, shrink-induced SH plastics are fabricated for a plethora of applications, including antibacterial applications, enhanced point-of-care (POC) detection, and reduced blood coagulation. Additionally, these purely structural SH surfaces are achieved in a roll-to-roll (R2R) platform for scalable manufacturing. Because their self-cleaning and water resistant properties, structurally modified SH surfaces prohibit bacterial growth and obviate bacterial chemical resistance. Antibacterial properties are demonstrated in a variety of SH plastics by preventing gram-negative Escherichia coli (E. coli) bacterial growth >150x compared to flat when fluid is rinsed and >20x without rinsing. Therefore, a robust and stable means to prevent bacteria growth is possible. Next, protein in urine is detected using a simple colorimetric output by evaporating droplets on a SH surface. Contrary to evaporation on a flat surface, evaporation on a SH surface allows fluid to dramatically concentrate because the weak adhesion constantly decreases the footprint area. On a SH surface, molecules in solution are confined to a footprint area 8.5x smaller than the original. By concentrating molecules, greater than 160x improvements in detection sensitivity are achieved compared to controls. Utility is demonstrated by detecting protein in urine in the pre-eclampsia range (150-300microgmL -1) for pregnant women. Further, SH surfaces repel bodily fluids including blood, urine, and saliva. Importantly, the surfaces minimize blood adhesion, leading to reduced blood coagulation without the need for anticoagulants. SH surfaces have >4200x and >28x reduction of blood residue area and volume compared to the non

  7. MetaGenSense: A web-application for analysis and exploration of high throughput sequencing metagenomic data [version 3; referees: 1 approved, 2 approved with reservations

    Directory of Open Access Journals (Sweden)

    Damien Correia

    2016-12-01

    Full Text Available The detection and characterization of emerging infectious agents has been a continuing public health concern. High Throughput Sequencing (HTS or Next-Generation Sequencing (NGS technologies have proven to be promising approaches for efficient and unbiased detection of pathogens in complex biological samples, providing access to comprehensive analyses. As NGS approaches typically yield millions of putatively representative reads per sample, efficient data management and visualization resources have become mandatory. Most usually, those resources are implemented through a dedicated Laboratory Information Management System (LIMS, solely to provide perspective regarding the available information. We developed an easily deployable web-interface, facilitating management and bioinformatics analysis of metagenomics data-samples. It was engineered to run associated and dedicated Galaxy workflows for the detection and eventually classification of pathogens. The web application allows easy interaction with existing Galaxy metagenomic workflows, facilitates the organization, exploration and aggregation of the most relevant sample-specific sequences among millions of genomic sequences, allowing them to determine their relative abundance, and associate them to the most closely related organism or pathogen. The user-friendly Django-Based interface, associates the users’ input data and its metadata through a bio-IT provided set of resources (a Galaxy instance, and both sufficient storage and grid computing power. Galaxy is used to handle and analyze the user’s input data from loading, indexing, mapping, assembly and DB-searches. Interaction between our application and Galaxy is ensured by the BioBlend library, which gives API-based access to Galaxy’s main features. Metadata about samples, runs, as well as the workflow results are stored in the LIMS. For metagenomic classification and exploration purposes, we show, as a proof of concept, that integration

  8. Magnetic red blood cells as new contrast agents for MRI applications

    Science.gov (United States)

    Antonelli, Antonella; Sfara, Carla; Manuali, Elisabetta; Salamida, Sonia; Louin, Gaëlle; Magnani, Mauro

    2013-03-01

    Superparamagnetic iron oxide (SPIO) nanoparticles have been produced and used successfully as potent contrast agents for Magnetic Resonance Imaging (MRI). However, a significant challenge associated with the biological application of SPIO-tracer agents is their behavior in vivo since their efficacy is often compromised due to a rapid recognition and clearance by the reticuloendothelial system (RES) which limits the applicability of such compounds in MRI. The advances in nanotechnology and molecular cell biology had lead to improve stability and biocompatibility of these nanoparticles, but despite a number of efforts, the SPIO half-life in blood circulation is very short. In this contest, the potential of red blood cells (RBCs) loaded with SPIO nanoparticles as a tracer material for MRI has been investigated in order to realize a blood pool tracer with longer blood retention time. Previously, we have proposed the encapsulation into RBCs of superparamagnetic iron oxide nanoparticles carboxydextran coated, such as Resovist contrast agent. This approach led to a nanoparticle reduction in uptake by the RES, increasing the blood circulation half-life of nanoparticles. Recently, the loading procedure was applied to a new contrast agent, the P904 ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles coated by hydrophilic derivatives of glucose, recently developed by Guerbet Laboratories. The results evidenced that this nanomaterial can be efficiently loaded into human and murine RBCs at concentrations ranging from 1.5 to 12 mM Fe. In vivo experiments performed in mice have showed an increased survival in the mouse vascular system of P904 encapsulated into RBCs respect to free P904 sample intravenously injected at the equivalent amounts.

  9. 76 FR 24850 - Notice of Request for a Revision of a Currently Approved Information Collection (Application for...

    Science.gov (United States)

    2011-05-03

    ..., national origin, gender, religion, age, disability, political beliefs, sexual orientation, and marital or... Web site provides the ability to type short comments directly into the comment field on this Web page... Exemptions. OMB Number: 0583-0082. Expiration Date of Approval: 07/31/2011. Type of Request: Revision of...

  10. 77 FR 40367 - Wyeth Pharmaceuticals, Inc.; Withdrawal of Approval of a New Drug Application for DURACT Capsules

    Science.gov (United States)

    2012-07-09

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Wyeth Pharmaceuticals, Inc.; Withdrawal of Approval of a New... DURACT (bromfenac sodium) Capsules, held by Wyeth Pharmaceuticals, Inc. (Wyeth), P.O. Box...

  11. 78 FR 46977 - Generic Drug User Fee-Abbreviated New Drug Application, Prior Approval Supplement, Drug Master...

    Science.gov (United States)

    2013-08-02

    ..., Prior Approval Supplement, Drug Master File, Final Dosage Form Facility, and Active Pharmaceutical... active pharmaceutical ingredient (API), and finished dosage form (FDF) facilities user fees related to... one or more finished dosage forms of a human generic drug or an active pharmaceutical ingredient...

  12. 78 FR 57857 - Medicare and Medicaid Programs; Application from the Compliance Team for Initial CMS-Approval of...

    Science.gov (United States)

    2013-09-20

    ... the Compliance Team for Initial CMS-Approval of its Rural Health Clinic Accreditation Program AGENCY... below, no later than 5 p.m. on October 21, 2013. ADDRESSES: In commenting, please refer to file code CMS... Services, Attention: CMS-3287-PN, P.O. Box 8016, Baltimore, MD 21244-8010. Please allow sufficient time...

  13. Electronic Approval of Invoices (AEF)

    CERN Multimedia

    2004-01-01

    With a view to the simplification of administrative procedures, AS and FI Departments have completed the second phase of the new procedure for the electronic approval of invoices via the EDH application. The aim of this new procedure is to rationalise the invoice approval process, notably by eliminating paper copies from the approval circuit. This will simplify the processing of invoices and facilitate their timely settlement, while at the same time maintaining a high level of security. Phase II includes handling the electronic approval process of invoices whose amounts do not correspond exactly to those of the associated orders. In such cases, budgetary approval is required for the entire invoiced amount. Further information can be obtained at : http://ais.cern.ch/projs/AEF/help/F_help.htm Phase II of the procedure will be introduced gradually with effect from April 2004. Finance Department, Accounts Payable Section Tel: 72295 Organisation and Procedures Tel: 75885 Information Technologies Department,...

  14. Rapid assessment of iron in blood plasma and serum by spectrophotometry with cloud-point extraction [version 1; referees: 2 approved

    OpenAIRE

    Tatyana Samarina; Mikhail Proskurnin

    2015-01-01

    Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II) complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5–6.3) is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure are higher sensitivity than the reference protocol: the limit of detection is 0.03 μg/mL, the limit ...

  15. Evolocumab: First Global Approval.

    Science.gov (United States)

    Markham, Anthony

    2015-09-01

    Evolocumab (Repatha™) is a fully human monoclonal antibody developed by Amgen that has been approved as a treatment for hypercholesterolaemia in the EU, and is awaiting approval in the USA and Japan. It specifically binds proprotein convertase subtilisin/kexin type 9 (PCSK9)-a negative regulator of low-density lipoprotein (LDL)-receptors-thereby improving the ability of the liver to bind LDL-cholesterol (LDL-C), leading to reduced LDL-C blood levels. The drug reduces LDL-C levels in patients with hypercholesterolaemia when used as monotherapy or in conjunction with a statin. This article summarizes the milestones in the development of evolocumab leading to this approval for the treatment of adults with primary hypercholesterolaemia (heterozygous familial and non-familial) or mixed dyslipidaemia as an adjunct to diet with or without a statin and/or other lipid lowering therapies, and in adults and adolescents aged ≥12 years with homozygous familial hypercholesterolaemia in combination with other lipid lowering therapies.

  16. Banking of pluripotent adult stem cells as an unlimited source for red blood cell production: potential applications for alloimmunized patients and rare blood challenges.

    Science.gov (United States)

    Peyrard, Thierry; Bardiaux, Laurent; Krause, Claire; Kobari, Ladan; Lapillonne, Hélène; Andreu, Georges; Douay, Luc

    2011-07-01

    The transfusion of red blood cells (RBCs) is now considered a well-settled and essential therapy. However, some difficulties and constraints still occur, such as long-term blood product shortage, blood donor population aging, known and yet unknown transfusion-transmitted infectious agents, growing cost of the transfusion supply chain management, and the inescapable blood group polymorphism barrier. Red blood cells can be now cultured in vitro from human hematopoietic, human embryonic, or human-induced pluripotent stem cells (hiPSCs). The highly promising hiPSC technology represents a potentially unlimited source of RBCs and opens the door to the revolutionary development of a new generation of allogeneic transfusion products. Assuming that in vitro large-scale cultured RBC production efficiently operates in the near future, we draw here some futuristic but realistic scenarios regarding potential applications for alloimmunized patients and those with a rare blood group. We retrospectively studied a cohort of 16,486 consecutive alloimmunized patients (10-year period), showing 1 to 7 alloantibodies with 361 different antibody combinations. We showed that only 3 hiPSC clones would be sufficient to match more than 99% of the 16,486 patients in need of RBC transfusions. The study of the French National Registry of People with a Rare Blood Phenotype/Genotype (10-year period) shows that 15 hiPSC clones would cover 100% of the needs in patients of white ancestry. In addition, one single hiPSC clone would meet 73% of the needs in alloimmunized patients with sickle cell disease for whom rare cryopreserved RBC units were required. As a result, we consider that a very limited number of RBC clones would be able to not only provide for the need for most alloimmunized patients and those with a rare blood group but also efficiently allow for a policy for alloimmunization prevention in multiply transfused patients.

  17. Forensic application of the luminol reaction as a presumptive test for latent blood detection.

    Science.gov (United States)

    Barni, Filippo; Lewis, Simon W; Berti, Andrea; Miskelly, Gordon M; Lago, Giampietro

    2007-05-15

    The forensic application of the luminol chemiluminescence reaction is reviewed. Luminol has been effectively employed for more than 40 years for the presumptive detection of bloodstains which are hidden from the naked eye at crime scenes and, for this reason, has been considered one of the most important and well-known assays in the field of forensic sciences. This review provides an historical overview of the forensic use of luminol, and the current understanding of the reaction mechanism with particular reference to the catalysis by blood. Operational use of the luminol reaction, including issues with interferences and the effect of the luminol reaction on subsequent serological and DNA testing is also discussed.

  18. 30 CFR 35.9 - Certificates of approval.

    Science.gov (United States)

    2010-07-01

    ... APPROVAL OF MINING PRODUCTS FIRE-RESISTANT HYDRAULIC FLUIDS General Provisions § 35.9 Certificates of approval. (a) Upon completion of an investigation of a hydraulic fluid MSHA will issue to the applicant... notification of approval will be issued. If a certificate of approval is issued, no test data or...

  19. Rapid assessment of iron in blood plasma and serum by spectrophotometry with cloud-point extraction [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Tatyana Samarina

    2015-08-01

    Full Text Available Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5–6.3 is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure are higher sensitivity than the reference protocol: the limit of detection is 0.03 μg/mL, the limit of quantitation is 0.1 μg/mL, the determination range is 0.1 – 2.8 μg/mL (RSD 0.02–0.10. Copper does not interfere with the iron assessment.

  20. Biofluid mechanical studies in models of blood vessels and some applications

    Science.gov (United States)

    Liepsch, D.

    1996-05-01

    Lasers are used in a wide variety of medical applications. While laser catheters have beem developed for highly accurate velocity measurement, these are invasive; noninvasive techniques are more desirable but not as precise. The laser is, however, a great tool for in vitro measurements. Several groups internationally are using the laser in the study of local velocity distribution in microscopic areas of specially constructed models. Laser Doppler anemometry is widely used to measure the local, time-dependent velocities, while phase Doppler anemometry has been developed to measure particle size, distribution and velocity. Most recently, laser analyzer techniques have been developed for analyzing the particle size of two phase flow systems. It has become increasingly important for physicians to visualize blood flow. In addition to the techniques mentioned above, several laser sheet techniques have been developed for precise measurements. This paper presents a short review of laser techniques and shows some applications especially for the laser-Doppler anemometer.

  1. Beam localization in HIFU temperature measurements using thermocouples, with application to cooling by large blood vessels.

    Science.gov (United States)

    Dasgupta, Subhashish; Banerjee, Rupak K; Hariharan, Prasanna; Myers, Matthew R

    2011-02-01

    Experimental studies of thermal effects in high-intensity focused ultrasound (HIFU) procedures are often performed with the aid of fine wire thermocouples positioned within tissue phantoms. Thermocouple measurements are subject to several types of error which must be accounted for before reliable inferences can be made on the basis of the measurements. Thermocouple artifact due to viscous heating is one source of error. A second is the uncertainty regarding the position of the beam relative to the target location or the thermocouple junction, due to the error in positioning the beam at the junction. This paper presents a method for determining the location of the beam relative to a fixed pair of thermocouples. The localization technique reduces the uncertainty introduced by positioning errors associated with very narrow HIFU beams. The technique is presented in the context of an investigation into the effect of blood flow through large vessels on the efficacy of HIFU procedures targeted near the vessel. Application of the beam localization method allowed conclusions regarding the effects of blood flow to be drawn from previously inconclusive (because of localization uncertainties) data. Comparison of the position-adjusted transient temperature profiles for flow rates of 0 and 400ml/min showed that blood flow can reduce temperature elevations by more than 10%, when the HIFU focus is within a 2mm distance from the vessel wall. At acoustic power levels of 17.3 and 24.8W there is a 20- to 70-fold decrease in thermal dose due to the convective cooling effect of blood flow, implying a shrinkage in lesion size. The beam-localization technique also revealed the level of thermocouple artifact as a function of sonication time, providing investigators with an indication of the quality of thermocouple data for a given exposure time. The maximum artifact was found to be double the measured temperature rise, during initial few seconds of sonication.

  2. 75 FR 65551 - Notice of Passenger Facility Charge (PFC) Approvals and Disapprovals

    Science.gov (United States)

    2010-10-25

    ... Authority, Wichita, Kansas. Application Number: 10-07-C-00-ICT. Application Type: Impose and use a PFC. PFC... Disapprovals. In September 2010, there were four applications approved. This notice also includes information on seven applications, one approved in May 2009, two approved in July 2010, and four approved...

  3. Application value comparison of peripheral blood and venous blood in blood routine examination%末梢血和静脉血在血常规检验中的应用价值比较

    Institute of Scientific and Technical Information of China (English)

    赖清连

    2015-01-01

    Objective:To explore the application value of peripheral blood and venous blood in blood routine examination.Methods:180 cases of healthy persons and patients with blood routine examination were selected.They were randomly divided into two groups.In the peripheral blood group,peripheral blood was collected to test.In the venous blood groups,venous blood was collected to test.We compared two groups of test results.Results:In peripheral blood group,hemoglobin,white blood cells and red blood cells were higher than those in the venous blood group,and the platelet count was lower than that of the venous blood group(P0.05).At 3 to 6 hours of detection,white blood cells,intermediate cells,red blood cells were significantly increased,and the platelet count was significantly decreased(P<0.05).Conclusion:The detection result of venous blood was more accurate,and it can be repeatedly detected,and it had obvious advantages compared with peripheral blood.Because the human body blood testing was affected by many factors,in order to avoid the error,the blood should be detected in 3 hours.%目的:探讨末梢血与静脉血在血常规检验中的应用价值。方法:收治行健康体检与接受血常规检验患者180例,随机分为两组,末梢血组采集末梢血进行检验,静脉血组采集静脉血进行检验,比较两组的检测结果。结果:末梢血组的血红蛋白、白细胞、红细胞等指标均高于静脉血组,血小板计数值低于静脉血组(P<0.05);两组即刻检测与2 h检测的各项指标均无明显差异(P>0.05),3~6 h检测,白细胞、中间细胞、红细胞等明显升高,血小板计数值明显降低(P<0.05)。结论:静脉血检测结果更为准确,且可重复检测,与末梢血相比,具有明显优势。由于人体血常规检测受多种因素影响,为了避免误差,应在3 h内进行检测。

  4. Differentiation of smooth muscle progenitor cells in peripheral blood and its application in tissue engineered blood vessels

    Institute of Scientific and Technical Information of China (English)

    Shang-zhe XIE; Ning-tao FANG; Shui LIU; Ping ZHOU; Yi ZHANG; Song-mei WANG; Hong-yang GAO; Luan-feng PAN

    2008-01-01

    Background: A major shortcoming in tissue engineered blood vessels (TEBVs) is the lack of healthy and easily attainable smooth muscle cells (SMCs). Smooth muscle progenitor cells (SPCs), especially from peripheral blood, may offer an alternative cell source for tissue engineering involving a less invasive harvesting technique. Methods: SPCs were isolated from 5-ml fresh rat peripheral blood by density-gradient centrifugation and cultured for 3 weeks in endothelial growth medium-2-MV (EGM-2-MV) medium containing platelet-derived growth factor-BB (PDGF BB). Before seeded on the synthesized scaffold, SPC-derived smooth muscle outgrowth cell (SOC) phenotypes were assessed by immuno-fluorescent staining, Western blot analysis, and reverse transcription polymerase chain reaction (RT-PCR). The cells were seeded onto the silk fibroin-modified poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (SF-PHBHHx) scaffolds by 6×104 cells/cm'2 and cultured under the static con-dition for 3 weeks. The growth and proliferation of the seeded cells on the scaffold were analyzed by 3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide (MTT) assay, scanning electron microscope (SEM), and 4,6-diamidino-2-phenylindole (DAPI) staining. Results: SOCs displayed specific "hill and valley" morphology, expressed the specific markers of the SMC lineage: protein, and extracellular matrix components elastin and matrix Gla protein (MGP), as well as vascular endothelial growth factor (VEGF). After seeded on the SF-PHBHHx scaffold, the cells showed excellent metabolic activity and proliferation. Conclusion: SPCs isolated from peripheral blood can be differentiated into the SMCs in vitro and have an impressive growth potential in the biodegradable synthesized scaffold. Thus, SPCs may be a promising cell source for constructing TEBVs.

  5. Drugs Approved for Melanoma

    Science.gov (United States)

    ... Ask about Your Treatment Research Drugs Approved for Melanoma This page lists cancer drugs approved by the ... that are not listed here. Drugs Approved for Melanoma Aldesleukin Cobimetinib Cotellic (Cobimetinib) Dabrafenib Dacarbazine DTIC-Dome ( ...

  6. Elosulfase alfa: first global approval.

    Science.gov (United States)

    Sanford, Mark; Lo, Jin Han

    2014-04-01

    Elosulfase alfa (Vimizim™) is a recombinant form of N-acetylgalactosamine-6-sulfatase (GALNS) that was developed by BioMarin Pharmaceutical Inc. as an enzyme replacement therapy for patients with mucopolysaccharidosis type IVA (MPS IVA), also known as Morquio A syndrome. Patients with MPS IVA have a GALNS deficiency, which results in serious musculoskeletal, cardiorespiratory and other system disturbances. Elosulfase alfa was approved by the US FDA on 14 February 2014 for the treatment of MPS IVA. The European Medicines Agency Committee for Medicinal Products for Human Use (CHMP) has recently recommended that elosulfase alfa be approved for use in the EU in the same indication. Within the last year, the manufacturer has also filed applications for approval for the use of elosulfase alfa in MPS IVA in Brazil, Australia, Canada and Mexico. This article summarizes the milestones in the development of elosulfase alfa leading to its first global approval in MPS IVA.

  7. [Research and Application of iPS Cells in Blood System].

    Science.gov (United States)

    Zhou, Li-Xia; Ye, Jie-Yu; Lian, Qi-Zhou; Yang, Mo

    2015-04-01

    Induced pluripotent stem cells (iPS cells) were first constructed by Takahshi and et al in 2006. They converted the mouse fibroblasts into ES-like cells via viral transduction with four transcription factors (Oct4, Sox2, Klf4 and c-Myc). Since, the significant progress has been made and many researchers have succeeded in inducing iPS cells from other human somatic cells by some novel approaches, such as combining transcriptional factors and small chemicals. IPS cells have significant prospect in clinical application. IPS cells derived from patient somatic cells can be used as a model in studying the pathogenesis of genetic hematological disease and applied in therapeutic screenings. Recent studies suggested that iPS cells can differentiate into red blood cells and platelets in vitro, which may make up a big blood bank for transfusion in future. In this review, current understanding of both recombinant technology of iPS cells and the research progress in hematology are summarized.

  8. Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells.

    Science.gov (United States)

    Giani, Felix C; Fiorini, Claudia; Wakabayashi, Aoi; Ludwig, Leif S; Salem, Rany M; Jobaliya, Chintan D; Regan, Stephanie N; Ulirsch, Jacob C; Liang, Ge; Steinberg-Shemer, Orna; Guo, Michael H; Esko, Tõnu; Tong, Wei; Brugnara, Carlo; Hirschhorn, Joel N; Weiss, Mitchell J; Zon, Leonard I; Chou, Stella T; French, Deborah L; Musunuru, Kiran; Sankaran, Vijay G

    2016-01-07

    Multipotent and pluripotent stem cells are potential sources for cell and tissue replacement therapies. For example, stem cell-derived red blood cells (RBCs) are a potential alternative to donated blood, but yield and quality remain a challenge. Here, we show that application of insight from human population genetic studies can enhance RBC production from stem cells. The SH2B3 gene encodes a negative regulator of cytokine signaling and naturally occurring loss-of-function variants in this gene increase RBC counts in vivo. Targeted suppression of SH2B3 in primary human hematopoietic stem and progenitor cells enhanced the maturation and overall yield of in-vitro-derived RBCs. Moreover, inactivation of SH2B3 by CRISPR/Cas9 genome editing in human pluripotent stem cells allowed enhanced erythroid cell expansion with preserved differentiation. Our findings therefore highlight the potential for combining human genome variation studies with genome editing approaches to improve cell and tissue production for regenerative medicine.

  9. Marketing Approval of Ethical Kampo Medicines.

    Science.gov (United States)

    Hakamatsuka, Takashi

    2017-01-01

     Kampo medicine is an original traditional medicine in Japan. Currently, 148 ethical Kampo formulations (Kampo prescription drugs) are registered in the National Health Insurance Price List. Kampo medicines can be prescribed under the national insurance system, which shows that they are part of conventional medicine in Japan. Japan has a unified drug approval system that does not distinguish between Western and Kampo medicines, and both are subject to the same regulations. The application for the market approval of ethical Kampo medicines is based on the general notification for drugs, i.e., "Handling of Ethical Combination Drugs" in "Precautions Necessary When Applying for Drug Marketing Approval" (Yakushokushinsa Notification No. 1121-12 of November 21, 2014). Furthermore, applications for the market approval of ethical Kampo medicines should follow the Kampo-specific notification of "Handling of Ethical Kampo Medicines" (Yakushin Notification No. 804 of June 25, 1980). Data from comparative studies with standard decoctions must be submitted with approval applications according to Yakushin 2 Notification No. 120 of May 31, 1985. The safety, efficacy, and quality of Kampo medicines are comprehensively assured by the Japanese Pharmacopoeia, Good Manufacturing Practice, Good Agricultural and Collection Practices, marketing approval certificate, approval standard, and pharmacovigilance. I believe that the basic framework for the market approval of ethical Kampo medicines has been established as described above. The key factors for the practical application of superior manufacturing technology and research achievements and the promotion of drug development are the specific guidelines for the approval of drugs of herbal origin.

  10. Distribution of blood derivatives by registered blood establishments that qualify as health care entities; Prescription Drug Marketing Act of 1987; Prescription Drug Amendments of 1992; delay of applicability date. Final rule; delay of applicability date.

    Science.gov (United States)

    2006-11-13

    The Food and Drug Administration (FDA) is further delaying, until December 1, 2008, the applicability date of a certain requirement of a final rule published in the Federal Register of December 3, 1999 (64 FR 67720) (the final rule). The final rule implements the Prescription Drug Marketing Act of 1987 (PDMA), as modified by the Prescription Drug Amendments of 1992 (PDA), and the Food and Drug Administration Modernization Act of 1997 (the Modernization Act). The provisions of the final rule became effective on December 4, 2000, except for certain provisions whose effective or applicability dates were delayed in five subsequent Federal Register notices, until December 1, 2006. The provision with the delayed applicability date would prohibit wholesale distribution of blood derivatives by registered blood establishments that meet the definition of a "health care entity." In the Federal Register of February 1, 2006 (71 FR 5200), FDA published a proposed rule specific to the distribution of blood derivatives by registered blood establishments that qualify as health care entities (the proposed rule). The proposed rule would amend certain limited provisions of the final rule to allow certain registered blood establishments that qualify as health care entities to distribute blood derivatives. In response to the proposed rule, FDA received substantive comments. As explained in the SUPPLEMENTARY INFORMATION section of this document, further delaying the applicability of Sec. 203.3(q) (21 CFR 203.3(q)) to the wholesale distribution of blood derivatives by health care entities is necessary to give the agency additional time to address comments on the proposed rule, consider whether regulatory changes are appropriate, and, if so, to initiate such changes.

  11. 78 FR 52430 - Withdrawal of Approval of New Animal Drug Applications; Quali-Tech Products, Inc.; Bambermycins...

    Science.gov (United States)

    2013-08-23

    ... Animal Drug Applications; Quali- Tech Products, Inc.; Bambermycins; Pyrantel; Tylosin; Virginiamycin... (pyrantel tartrate), NADA 132-705 for FLAVOMYCIN (bambermycins), and NADA 133-335 for STAFAC...

  12. 5 CFR 630.1107 - Notification of approval or disapproval of an application to become an emergency leave recipient.

    Science.gov (United States)

    2010-01-01

    ... of an application to become an emergency leave recipient. 630.1107 Section 630.1107 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS ABSENCE AND LEAVE Emergency Leave... emergency leave recipient. Once the employee's application to become an emergency leave recipient is...

  13. Blood Types

    Science.gov (United States)

    ... maternity. Learn About Blood Blood Facts and Statistics Blood Components Whole Blood and Red Blood Cells Platelets Plasma ... About Blood Blood Facts and Statistics Blood Types Blood Components What Happens to Donated Blood Blood and Diversity ...

  14. Parameter estimation and actuator characteristics of hybrid magnetic bearings for axial flow blood pump applications.

    Science.gov (United States)

    Lim, Tau Meng; Cheng, Shanbao; Chua, Leok Poh

    2009-07-01

    Axial flow blood pumps are generally smaller as compared to centrifugal pumps. This is very beneficial because they can provide better anatomical fit in the chest cavity, as well as lower the risk of infection. This article discusses the design, levitated responses, and parameter estimation of the dynamic characteristics of a compact hybrid magnetic bearing (HMB) system for axial flow blood pump applications. The rotor/impeller of the pump is driven by a three-phase permanent magnet brushless and sensorless motor. It is levitated by two HMBs at both ends in five degree of freedom with proportional-integral-derivative controllers, among which four radial directions are actively controlled and one axial direction is passively controlled. The frequency domain parameter estimation technique with statistical analysis is adopted to validate the stiffness and damping coefficients of the HMB system. A specially designed test rig facilitated the estimation of the bearing's coefficients in air-in both the radial and axial directions. Experimental estimation showed that the dynamic characteristics of the HMB system are dominated by the frequency-dependent stiffness coefficients. By injecting a multifrequency excitation force signal onto the rotor through the HMBs, it is noticed in the experimental results the maximum displacement linear operating range is 20% of the static eccentricity with respect to the rotor and stator gap clearance. The actuator gain was also successfully calibrated and may potentially extend the parameter estimation technique developed in the study of identification and monitoring of the pump's dynamic properties under normal operating conditions with fluid.

  15. Closed system generation of dendritic cells from a single blood volume for clinical application in immunotherapy.

    Science.gov (United States)

    Elias, M; van Zanten, J; Hospers, G A P; Setroikromo, A; de Jong, M A; de Leij, L F M H; Mulder, N H

    2005-12-01

    Dendritic cells (DC) used for clinical trials should be processed on a large scale conforming to current good manufacturing practice (cGMP) guidelines. The aim of this study was to develop a protocol for clinical grade generation of immature DC in a closed-system. Aphereses were performed with the Cobe Spectra continuous flow cell separator and material was derived from one volume of blood processed. Optimisation of a 3-phase collection autoPBSC technique significantly improved the quality of the initial mononuclear cell (MNC) product. Monocytes were then enriched from MNC by immunomagnetic depletion of CD19+ B cells and CD2+ T cells and partial depletion of NK cells using the Isolex 300I Magnetic cell selector. The quality of the initial mononuclear cell product was found to determine the outcome of monocyte enrichment. Enriched monocytes were cultured in Opticyte gas-permeable containers using CellGro serum-free medium supplemented with GM-CSF and IL-4 to generate immature DC. A seeding concentration of 1 x 10(6) was found optimal in terms of DC phenotype expression, monocyte percentage in culture, and cell viability. The differentiation pattern favours day 7 for harvest of immature DC. DC recovery, viability, as well as phenotype expression after cryopreservation of immature DC was considered in this study. DC were induced to maturation and evaluated in FACS analysis for phenotype expression and proliferation assays. Mature DC were able to generate an allogeneic T-cell response as well as an anti-CMV response as detected by proliferation assays. These data indicate that the described large-scale GMP-compatible system results in the generation of stable DC derived from one volume of blood processed, which are qualitatively and quantitatively sufficient for clinical application in immunotherapeutic protocols.

  16. Thermal Blood Clot Formation and use in Microfluidic Device Valving Applications

    Science.gov (United States)

    Tai, Yu-Chong (Inventor); Shi, Wendian (Inventor); Guo, Luke (Inventor)

    2014-01-01

    The present invention provides a method of forming a blood-clot microvalve by heating blood in a capillary tube of a microfluidic device. Also described are methods of modulating liquid flow in a capillary tube by forming and removing a blood-clot microvalve.

  17. 24 CFR 214.103 - Approval criteria.

    Science.gov (United States)

    2010-04-01

    ... counseling staff must possess a working knowledge of HUD's housing and single-family mortgage insurance... COUNSELING PROGRAM Approval and Disapproval of Housing Counseling Agencies § 214.103 Approval criteria. The... counseling agencies, branches, and affiliates that are included in one application: (a) Nonprofit and...

  18. 5 CFR 890.203 - Application for approval of, and proposal of amendments to, health benefit plans.

    Science.gov (United States)

    2010-01-01

    ... 20415. Application letters must be accompanied by any descriptive material, financial data, or other documentation required by OPM. Plans must submit the letter and attachments in the OPM-specified format by... to an expected break-even point including a sufficient amount for unexpected contingencies;...

  19. 77 FR 65198 - Generic Drug User Fee-Abbreviated New Drug Application, Prior Approval Supplement, and Drug...

    Science.gov (United States)

    2012-10-25

    ... applications in the backlog as of October 1, 2012, on finished dosage form (FDF) and active pharmaceutical ingredient (API) facilities, and on type II active pharmaceutical ingredient DMFs to be made available for... pharmaceutical ingredients other than by reference to a DMF will pay an additional fee that is based on...

  20. Optical methods for correction of oxygen-transport characteristics of blood and their biomedical applications

    Science.gov (United States)

    Zalesskaya, G. A.; Akulich, N. V.; Marochkov, A. V.; Laskina, O. V.; Mit'kovskaya, N. P.

    2010-07-01

    We have carried out a comprehensive analysis of the spectral characteristics of blood and blood components, gas-exchange and oximetry parameters for venous and arterial blood, central hemodynamic parameters, and the results of a complete blood count and chemistry panel before and after extracorporeal UV irradiation of the blood (UBI, ultraviolet blood irradiation) or intravenous exposure of blood to low-intensity emission from an He-Ne laser (LBI, laser blood irradiation). We have demonstrated the possibility of correcting the oxygentransport characteristics of blood by laser optical methods based on photodissociation of blood oxyhemoglobin. We have shown that the therapeutic effects initiated both by UBI and LBI are based on a single mechanism: a change in the balance between production of active oxygen species and their inhibition by antioxidants. The data obtained are of interest not only for studying the primary (molecular) mechanisms of action for photohemotherapy and their effect on processes occurring in the living body, but also can provide a basis for designing next-generation laser optical instruments and for development of not yet existing methods for assessing the therapeutic efficacy of photohemotherapy.

  1. Chlorpyrifos Determined in Human Blood by UPLC-MS/MS and Its Application in Poisoning Cases

    Institute of Scientific and Technical Information of China (English)

    QIAO Zheng; YAN Hui; ZHUO Xian-yi; SHEN Bao-hua

    2015-01-01

    Objective To determine the chlorpyrifos in human blood by liquid chromatography-tandemmass spectrometry and to validate its application in poisoning cases. Methods The samples were extracted by a simple one-step protein precipitation procedure. Chromatography was performed on a Capcell Pack C18 mG II column (250 mm×2.0 mm, 5μm) using an isocratic elution of solvent A (0.1% formic acid-water with 2 mmol/L ammoniumacetate) and solvent B (methanol with 2 mmol/L ammoniumacetate) at 5∶95 (V∶V).Results The linearranged from5 to 500ng/mL (r=0.9987).Thelimitofdetection (LOD) and the lower limit of quantification (LLOQ ) were 2 ng/mL and 4 ng/mL , respectively. For this method, the precision and accuracy of intra-day and inter-day were <10% and 97.44%-101.10%, respectively. The re-sults in stability test of long-termfrozen were satisfied. The matrix effect, recovery and process efficien-cy were 64.97%-86.81%, 76.70%-85.52%, and 55.57%-66.58%, respectively. Conclusion This method can provide a rapid approach to chlorpyrifos extraction and determination in toxicological analysis of forensic and clinical treatment.

  2. Design and parameter estimation of hybrid magnetic bearings for blood pump applications

    Science.gov (United States)

    Lim, Tau Meng; Zhang, Dongsheng; Yang, Juanjuan; Cheng, Shanbao; Low, Sze Hsien; Chua, Leok Poh; Wu, Xiaowei

    2009-10-01

    This paper discusses the design and parameter estimation of the dynamics characteristics of a high-speed hybrid magnetic bearings (HMBs) system for axial flow blood pump applications. The rotor/impeller of the pump is driven by a three-phase permanent magnet (PM) brushless and sensorless DC motor. It is levitated by two HMBs at both ends in five-degree-of-freedom with proportional-integral-derivative (PID) controllers; among which four radial directions are actively controlled and one axial direction is passively controlled. Test results show that the rotor can be stably supported to speeds of 14,000 rpm. The frequency domain parameter estimation technique with statistical analysis is adopted to validate the stiffness and damping coefficients of the HMBs system. A specially designed test rig facilitated the estimation of the bearing's coefficients in air—in both the radial and axial directions. The radial stiffness of the HMBs is compared to the Ansoft's Maxwell 2D/3D finite element magnetostatic results. Experimental estimation showed that the dynamics characteristics of the HMBs system are dominated by the frequency-dependent stiffness coefficients. The actuator gain was also successfully calibrated and may potentially extend the parameter estimation technique developed in the study of identification and monitoring of the pump's dynamics properties under normal operating conditions with fluid.

  3. The analysis of economic capital application of credit approval in commercial banks%浅析经济资本在商业银行授信审批中的应用

    Institute of Scientific and Technical Information of China (English)

    胡晓磊; 刘庆卫

    2014-01-01

    This paper firstly analyzed the problems exposed in current commercial bank credit approval, then analyzed the significance of economic capital management in credit approval stage, and finally put forward some thoughts and suggestion for the application of economic capital approach in credit approval process.%本文结合当前商业银行授信审批中暴露出的问题,分析了在授信审批阶段科学应用经济资本管理工具的重要意义,提出了在授信审批环节应用经济资本的思路及建议。

  4. 输血管理系统在临床输血中的研发与应用%Development and Application of Transfusion Management System in Clinical Blood Transfrusion

    Institute of Scientific and Technical Information of China (English)

    邢月利; 王艳春; 王守燕; 张力

    2014-01-01

    divided into data-non management group (n =9 960) and data management group (n =12 432).Comprehensive improvement rate,blood approval rate and transfusion consent signed rate in medical record; and requisition pass rate,specimen correct rate,crossmatch error rate,accurate rate of blood check and adverse transfusion reactions feedback rate in blood data were compared before and after the application of transfusion management system,respectively.Results After use of the blood transfusion management system,the comprehensive improvement rate and blood approval rate were significantly improved,compared with the data before this system using,the differences were statistically significant (x2 =58.17,904.80; P<0.05).Requisition pass rate,specimens correct rate,blood transfusion adverse feedback rate in data-management group were higher than the data-non management group,the differences in these two groups had statistically significant (x2 =2 053.28,8.74,408.28; P < 0.05).Conclusions Application of transfusion management system could effectively improve the quality of terminal medical records,ensure the implementation of blood approval procedures,improve blood transfusion apply acceptable level,achieve comprehensive monitoring of blood clinical use,build information platform,and promote scientific and rational use of blood.

  5. Phototherapies: photosensitized inactivation of viral and protozoan infectious agents and potential application in blood banking

    Science.gov (United States)

    Judy, Millard M.; Matthews, James Lester; Sogandares-Bernal, Franklin M.; Newman, Joseph T.; Chanh, Tran C.; Marengo-Rowe, Alain J.

    1992-06-01

    More than 10 million units of human blood components are processed annually in the United States. Although donor screening and testing have greatly lowered the risk of transmission of viral and protozoan infectious agents, additional sterilization procedures which also preserve blood component function would be of significant value. Use of UV-A and visible-light-range photosensitizers for sterilization of blood platelets and red blood cells, respectively, is currently being aggressively investigated in laboratory-scale optical-mechanical systems. With successful demonstration of the efficacy and safety of these sterilization techniques, implementation in the blood bank setting will require scale-up to optical-mechanical systems capable of handling approximately 25,000 units daily in 500 - 1,000 blood banks in the United States.

  6. Innovative Applications of Robotic Surgery: Renal Allograft and Autologous Transplantation [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Jason Lee

    2016-01-01

    Full Text Available Robotic surgery has enabled surgeons to offer more patients a minimally invasive surgical option in the management of their complex diseases. While renal transplantation is associated with significant improvements in quantity and quality of life for most end-stage renal disease (ESRD patients, it is also not devoid of its surgical risks and potential morbidities. Robotic-assisted kidney transplantation is a recently described, innovative application of the robotic surgery platform, and early experiences suggest that it is associated with comparable graft function and lower rates of complications. Urinary tract obstruction, though less common than ESRD, can be a serious threat to renal function. Severe ureteric stricture disease can represent a clinically complex problem requiring major reconstructive surgery. Completely intra-corporeal robotic renal auto-transplantation is another innovative application of the robotic surgery platform and represents a significant advancement in urologic surgery. Initial reports of this procedure demonstrate safety, feasibility, and excellent renal function outcomes.

  7. The application of blood flow measurements to the study of aging muscle.

    Science.gov (United States)

    McCully, K K; Posner, J D

    1995-11-01

    Blood flow to skeletal muscle is a potentially important factor in the reduction of muscle function associated with aging (sarcopenia). The main influence of reduced blood flow capacity on muscle function is in limiting oxidative metabolism. Direct measures of blood flow include: intravital-microscopy, plethysmography, radioactive microspheres, 133Xenon washout, thermodilution, and Doppler ultrasound. Indirect measurement of blood flow includes arm-to-ankle pressure index and the rate of phosphocreatine recovery after exercise. Several new methodologies have been developed to evaluate muscle blood flow, including color-Doppler imaging, magnetic resonance imaging/angiography (MRI/MRA), and near-infrared spectroscopy (NIRS). As adaptations of traditional techniques, these methods promise more precise information under less invasive conditions. MRI is an expensive and technically challenging method able to measure vessel location, blood flow, and wall diameter in blood vessels throughout the cardiac cycle. Color-Doppler provides excellent temporal resolution blood flow throughout the cardiac cycle, along with some anatomical information. NIRS is an inexpensive and portable technology that can measure changes in oxygen saturation and provide information on tissue oxygen delivery in studies of frailer and more difficult-to-study subjects. Muscle blood flow is not thought to limit oxidative metabolism under normal conditions in young individuals. However, it is not clear what happens to muscle blood flow in healthy older individuals. Reduced capillary density, less maximal blood flow, and a slower hyperemic flow response have been reported in some, but not all, studies. Further studies with the newer methodologies are needed to re-examine age-related changes in muscle blood flow.(ABSTRACT TRUNCATED AT 250 WORDS)

  8. Electronic Approval of Invoices (AEF)

    CERN Multimedia

    2003-01-01

    With a view to the simplification of administrative procedures, AS and FI Divisions have drawn up a new procedure for the electronic approval of invoices via the EDH application. The aim of this new procedure is to rationalise the invoice approval process, notably by eliminating paper copies from the approval circuit. This will simplify the processing of invoices and facilitate their timely settlement, while at the same time maintaining a high level of security. This new procedure, in its current phase, will be gradually implemented from 1 November 2003 onwards. For clarification and further information, please see: http://ais.cern.ch/projs/AEF/help/help.htm . Finance Division, Accounts Payable Tel.: 7.22.95

  9. Applicability of three commercially available kits for forensic identification of blood stains.

    Science.gov (United States)

    Horjan, Ivana; Barbaric, Lucija; Mrsic, Gordan

    2016-02-01

    Various commercially available one-step immunoassays for detection of human (primate) blood have been developed. This study evaluated two hemoglobin tests, ABAcard(®) HemaTrace(®) and HemDirect Hemoglobin against glycophorin A test-RSID™-Blood for following parameters: sensitivity, specificity, effectiveness using various substrates, stain remover and aged blood stains. The highest blood detection limit was observed if HemaTrace(®) was used. When compared with HemaTrace(®), ten times lower sensitivity was observed for HemDirect Hemoglobin test. No false positives were obtained for HemDirect Hemoglobin while ABAcard(®) HemaTrace(®), probably due to its extreme sensitivity, showed high percent of false positives with saliva. The lowest sensitivity and 40% of false positives with saliva was exhibited by RSID™-Blood. In addition, this test encountered the lowest efficacy if aged blood-stains or blood treated with stain remover were used. As expected, none of the tested substrates (wood, metal, brick, and soil), influenced on blood testing, although soil substrate affected STR amplification. Conducted studies established HemDirect Hemoglobin test as more reliable for evaluated parameters than ABAcard(®) HemaTrace(®) and RSID™-Blood.

  10. Estimation of indocyanine green concentration in blood from fluorescence emission: application to hemodynamic assessment during hemodialysis

    Science.gov (United States)

    Maarek, Jean-Michel I.; Holschneider, Daniel P.

    2009-09-01

    There is considerable interest in assessing cardiovascular function noninvasively in patients receiving hemodialysis. A possible approach is to measure the blood concentration of bolus-injected indocyanine green dye and to apply the dye-dilution method for estimating cardiac output and blood volume. Blood ICG concentration can be derived from a measurement of the ICG fluorescence through the dialysis tubing if a simple and unique calibration relationship can be established between transmural fluorescence intensity and blood ICG concentration. We investigated this relationship using Monte Carlo simulations of light transport in blood with varying hematocrit and ICG concentrations and performed empiric measurements of optical absorption and ICG fluorescence emission to confirm our findings. The ICG fluorescence intensity measured at the blood surface, as well as the light intensity remitted by the blood, varied as hematocrit changes modified the absorption and scattering characteristics of the blood. Calibration relationships were developed between fluorescence intensity and ICG concentration that accounted for hematocrit changes. Combining the backreflected fluorescence and the reflected light measured near the point of illumination provided optimal signal intensity, linearity, and robustness to hematocrit changes. These results provide a basis for developing a noninvasive approach to derive optically circulating blood ICG concentration in hemodialysis circuits.

  11. Near-infrared spectroscopy measurement of blood oxygenation content and its application in sports practice

    Science.gov (United States)

    Xu, Guodong; Gong, Hui; Ge, Xinfa; Luo, Qingming

    2003-12-01

    To research the change characteristics of blood oxygenation content in skeletal muscle, the change regularity between blood oxygenation content and exercise intensity as well as HbO2 and blood lactate acid while taking incremental exercises, we took an in vivo, real-time and continuous measurement on the blood oxygenation content of eight sportsmen when they did incremental exercises of five degrees on a power bicycle using a portable tissue oximeter which is based on the principle of near-infrared spectroscopy(NIRS), simultaneously, we detected the blood lactate acid of subjects after each degree of incremental physical load instantly using a blood lactate analysis equipment. The results showed that the content of HbO2 descended regularly while that of Hb ascended; blood volume decreased; and the density of lactate increased as the intensity of exercises was heightened. The statistics analyses showed that the relationship between HbO2 and blood lactate is rather close (correlation coefficient r=-0.918). With this discovery, a theoretical basis in measuring the relative change of blood oxygenation content non-invasively was evidenced, and a novel technology for assessing the physical situation of sportsman, grasping sports density and evaluating the training effect could be imported.

  12. Topical application of THC containing products is not able to cause positive cannabinoid finding in blood or urine.

    Science.gov (United States)

    Hess, C; Krämer, M; Madea, B

    2017-03-01

    A male driver was checked during a traffic stop. A blood sample was collected 35min later and contained 7.3ng/mL THC, 3.5ng/mL 11-hydroxy-THC and 44.6ng/mL 11-nor-9-carboxy-THC. The subject claimed to have used two commercially produced products topically that contained 1.7ng and 102ng THC per mg, respectively. In an experiment, three volunteers (25, 26 and 34 years) applied both types of salves over a period of 3days every 2-4h. The application was extensive (50-100cm(2)). Each volunteer applied the products to different parts of the body (neck, arm/leg and trunk, respectively). After the first application blood and urine samples of the participants were taken every 2-4h until 15h after the last application (overall n=10 urine and n=10 blood samples, respectively, for each participant). All of these blood and urine samples were tested negative for THC, 11-hydroxy-THC and 11-nor-9-carboxy-THC by a GC-MS method (LoD (THC)=0.40ng/mL; LoD (11-hydroxy-THC)=0.28ng/mL; LoD (THC-COOH)=1.6ng/mL;. LoD (THC-COOH in urine)=1.2ng/mL). According to our studies and further literature research on in vitro testing of transdermal uptake of THC, the exclusive application of (these two) topically applied products did not produce cannabinoid findings in blood or urine.

  13. Post-Approval Studies

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CDRH Post-Approval Studies Program encompasses design, tracking, oversight, and review responsibilities for studies mandated as a condition of approval of a...

  14. 50 CFR 15.26 - Approval of cooperative breeding programs.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 1 2010-10-01 2010-10-01 false Approval of cooperative breeding programs... WILDLIFE AND PLANTS WILD BIRD CONSERVATION ACT Permits and Approval of Cooperative Breeding Programs § 15.26 Approval of cooperative breeding programs. Upon receipt of a complete application, the...

  15. Application of transcutaneous diffuse reflectance spectroscopy in the measurement of blood glucose concentration

    Institute of Scientific and Technical Information of China (English)

    Wenliang Chen(陈文亮); Rong Liu(刘蓉); Houxin Cui(崔厚欣); Kexin Xu(徐可欣); Lina Lü(吕丽娜)

    2004-01-01

    In this paper, the propagation characteristics of near-infrared (NIR) light in the palm tissue are analyzed,and the principle and feasibility of using transcutaneous diffuse reflectance spectroscopy for non-invasive blood glucose detection are presented. An optical probe suitable for measuring the diffuse reflectance spectrum of human palm and a non-invasive blood glucose detection system using NIR spectroscopy are designed. Based on this system, oral glucose tolerance tests are performed to measure the blood glucose concentrations of two young healthy volunteers. The partial least square calibration model is then constructed by all individual experimental data. The final result shows that correlation coefficients of the two experiments between the predicted blood glucose concentrations and the reference blood glucose concentrations are 0.9870 and 0.9854, respectively. The root mean square errors of prediction of full cross validation are 0.54 and 0.52 mmol/1, respectively.

  16. Clinical application of /sup 99m/Tc-HM-PAO for cerebral blood flow imaging by SPECT. Comparison with cerebral blood flow study by PET

    Energy Technology Data Exchange (ETDEWEB)

    Inugami, Atsushi; Uemura, Kazuo; Shishido, Fumio; Tomura, Noriaki; Higano, Shuichi; Fujita, Hideaki; Kanno, Iwao

    1988-02-01

    Recently, a new tracer for cerebral blood flow (CBF) study; /sup 99m/Tc-labelled-hexamethyl-propyleneamine-oxime (/sup 99m/Tc-HM-PAO) was developed by Amersham international institute. In this paper, we reported the initial experience of tomographic CBF imaging with /sup 99m/Tc-HM-PAO in the comparison of CBF study using positron CT (PET) and 0 approx. 15 labelled CO/sub 2/. Thirty-nine patients with cerebro-vascular disease were examined mainly in the acute phase. All the subjects showed verious disturbances of CBF, which corresponded well to the PET study. However, the image-contrast with /sup 99m/Tc-HM-PAO were inferior to those of the PET study. /sup 99m/Tc-HM-PAO is considered to a good radiopharmaceutical which is readily applicable to eaven an emergency cases.

  17. Aromatic DNA adducts in human white blood cells and skin after dermal application of coal tar

    Energy Technology Data Exchange (ETDEWEB)

    Godschalk, R.W.L.; Ostertag, J.U.; Moonen, E.J.C.; Neumann, H.A.M.; Kleinjans, J.C.S.; Schooten, F.J. van [University of Maastricht, Maastricht (Netherlands). Dept. of Health Risk Analysis and Toxicology

    1998-09-01

    A group of eczema patients topically treated with coal tar (CT) ointments was used as a model population to examine the applicability of DNA adducts in white blood cell (WBC) subpopulations as a measure of dermal exposure to polycyclic aromatic hydrocarbons (PAHs). Aromatic DNA adducts were examined by {sup 32}P-postlabeling in exposed skin and WBC subsets, and urinary excretion of PAH metabolites was determined to assess the whole-body burden. The median urinary excretion of 1-hydroxypyrene and 3-hydroxybenzo(a)pyrene was 0.39 and 0.01 {mu}mol/mol creatinine respectively, before the dermal application of CT ointments. After treatment for 1 week, these levels increased to 139.7 and 1.18 {mu}mol/mol creatinine respectively, indicating that considerable amounts of PAHs were absorbed. Median aromatic DNA adduct levels were significantly increased in skin from 2.9 adduct/10{sup 8} nucleotides before treatment to 63.3 adducts/10{sup 8} nt after treatment with CT, in monocytes from 0.28 to 0.86 adducts/10{sup 8} nt, in lymphocytes from 0.33 to 0.89 adducts/10{sup 8} nt and in granulocytes from 0.28 to 0.54 adducts/10{sup 8} nt. A week after stopping the CT treatment, the DNA adduct levels in monocytes and granulocytes were reduced to 0.38 and 0.38 adducts/10{sup 8} nt respectively, whereas the adduct levels in lymphocytes remained enhanced. Total DNA adduct levels in skin correlated with the adduct levels in monocytes and lymphocytes. Excretion of urinary metabolites during the first week of treatment was correlated with the percentage of the skin surface treated with CT ointment and decreased within a week after the cessation of treatment. 3-Hydroxybenzo(a)pyrene excretion, correlated with the levels of DNA adducts in skin that comigrated with benzo(a)pyrene-diol-epoxide-DNA. This study indicates that the DNA adduct levels in mononuclear WBCs can possibly be used as a surrogate for skin DNA after dermal exposure to PAHs. 34 refs., 4 figs., 1 tab.

  18. Applicability of bacterial endotoxins test to various blood products by the use of endotoxin-specific lysates.

    Science.gov (United States)

    Ochiai, Masaki; Yamamoto, Akihiko; Naito, Seishiro; Maeyama, Jun-Ichi; Masumi, Atsuko; Hamaguchi, Isao; Horiuchi, Yoshinobu; Yamaguchi, Kazunari

    2010-11-01

    Endotoxin contamination is a serious threat to the safety of parenteral drugs, and the rabbit pyrogen test has played a crucial role in controlling this contamination. Although the highly sensitive endotoxin test has replaced the pyrogen test for various pharmaceuticals, the pyrogen test is still implemented as the control test for most blood products in Japan. We examined the applicability of the endotoxin test to blood products for reliable detection and quantification of endotoxin. Nineteen types of blood products were tested for interfering factors based on spike/recovery of endotoxin by using 2 types of endotoxin-specific lysate reagents for photometric techniques. Interfering effects on the endotoxin test by the products could be eliminated by diluting from 1/2 to 1/16, with the exception of antithrombin III. However, conventional lysate reagents that also react with non-pyrogenic substances, such as (1-3)-β-D-glucan, produced results that were not relevant to endotoxin content or pyrogenicity. Our results showed that the endotoxin test would be applicable to most blood products if used with appropriate endotoxin-specific lysate reagents.

  19. Biosimilars approval process.

    Science.gov (United States)

    Zuñiga, Leyre; Calvo, Begoña

    2010-04-01

    For similar biological medicinal products, the so-called biosimilars, clinical trials are required rather than just the bioequivalence studies required to support the registration of a generic small molecule drug product. The EU Directive 2001/83/EC, as amended, stated that where a biological medicinal product which is similar to a reference biological product, does not meet the conditions in the definition of generic medicinal products the results of appropriate pre-clinical tests or clinical trials relating to these conditions must be provided. The challenge is to determine the exact nature of the non-clinical and clinical programme required to gain regulatory approval. The applicant is encouraged to provide a detailed description of the strategy used to demonstrate the biosimilar and the reference product have similar profiles in terms of quality, safety and efficacy. The extent to which comparability can be proven will have quite an impact on how many non-clinical and clinical studies the biosimilar applicant will be required to conduct. The dossier submitted by the applicant to the EMEA should cover all aspects of the comparability assessment and must include data on possible unwanted immune reactions to the therapeutic protein. Post-marketing pharmacovigilance plans are also expected to be included in the biosimilar dossier.

  20. Correspondence of DNA Methylation Between Blood and Brain Tissue and Its Application to Schizophrenia Research.

    Science.gov (United States)

    Walton, Esther; Hass, Johanna; Liu, Jingyu; Roffman, Joshua L; Bernardoni, Fabio; Roessner, Veit; Kirsch, Matthias; Schackert, Gabriele; Calhoun, Vince; Ehrlich, Stefan

    2016-03-01

    Given the difficulty of procuring human brain tissue, a key question in molecular psychiatry concerns the extent to which epigenetic signatures measured in more accessible tissues such as blood can serve as a surrogate marker for the brain. Here, we aimed (1) to investigate the blood-brain correspondence of DNA methylation using a within-subject design and (2) to identify changes in DNA methylation of brain-related biological pathways in schizophrenia.We obtained paired blood and temporal lobe biopsy samples simultaneously from 12 epilepsy patients during neurosurgical treatment. Using the Infinium 450K methylation array we calculated similarity of blood and brain DNA methylation for each individual separately. We applied our findings by performing gene set enrichment analyses (GSEA) of peripheral blood DNA methylation data (Infinium 27K) of 111 schizophrenia patients and 122 healthy controls and included only Cytosine-phosphate-Guanine (CpG) sites that were significantly correlated across tissues.Only 7.9% of CpG sites showed a statistically significant, large correlation between blood and brain tissue, a proportion that although small was significantly greater than predicted by chance. GSEA analysis of schizophrenia data revealed altered methylation profiles in pathways related to precursor metabolites and signaling peptides.Our findings indicate that most DNA methylation markers in peripheral blood do not reliably predict brain DNA methylation status. However, a subset of peripheral data may proxy methylation status of brain tissue. Restricting the analysis to these markers can identify meaningful epigenetic differences in schizophrenia and potentially other brain disorders.

  1. Application of optical lens of a CD writer for detecting the blood glucose semi-invasively

    Science.gov (United States)

    Meshram, N. D.; Dahikar, P. B.

    2014-10-01

    Recent technological advancements in the photonics industry have led to a resurgence of interest in optical glucose sensing and to realistic progress toward the development of an optical glucose sensor. Such a sensor has the potential to significantly improve the quality of life for the estimated 16 million diabetics in this country by making routine glucose measurements more convenient. Currently over 100 small companies and universities are working to develop noninvasive or minimally invasive glucose sensing technologies, and optical methods play a large role in these efforts. It has become overwhelmingly clear that frequent monitoring and tight control of blood sugar levels are requisite for effective management of Diabetes mellitus and reduction of the complications associated with this disease. The pain and trouble associated with current "finger-stick" methods for blood glucose monitoring result in decreased patient compliance and a failure to control blood sugar levels. Thus, the development of a convenient noninvasive blood glucose monitor holds the potential to significantly reduce the morbidity and mortality associated with Diabetes. A method and apparatus for noninvasive measurement of blood glucose concentration based on transilluminated laser beam via the Index Finger has been reported in this paper. This method depends on photodiode based laser operating at 632.8 nm wavelength. During measurement, the index finger is inserted into the glucose sensing unit, the transilluminated optical signal is converted into an electrical signal, compared with the reference electrical signal, and the obtained difference signal is processed by signal processing unit which presents the results in the form of blood glucose concentration. This method would enable the monitoring blood glucose level of the diabetic patient continuously, safely and noninvasively..

  2. Application of optical lens of a CD writer for detecting the blood glucose semi-invasively

    Energy Technology Data Exchange (ETDEWEB)

    Meshram, N. D., E-mail: meshramnileshsd@gmail.com [Mathuradas Mohota College of Sciences, Nagpur-440009 (India); Dahikar, P. B., E-mail: pbdahikar@rediffmail.com [Kamla Nehru Mahavidyalaya, Sakkardara Square, Nagpur-440009 (India)

    2014-10-15

    Recent technological advancements in the photonics industry have led to a resurgence of interest in optical glucose sensing and to realistic progress toward the development of an optical glucose sensor. Such a sensor has the potential to significantly improve the quality of life for the estimated 16 million diabetics in this country by making routine glucose measurements more convenient. Currently over 100 small companies and universities are working to develop noninvasive or minimally invasive glucose sensing technologies, and optical methods play a large role in these efforts. It has become overwhelmingly clear that frequent monitoring and tight control of blood sugar levels are requisite for effective management of Diabetes mellitus and reduction of the complications associated with this disease. The pain and trouble associated with current “finger-stick” methods for blood glucose monitoring result in decreased patient compliance and a failure to control blood sugar levels. Thus, the development of a convenient noninvasive blood glucose monitor holds the potential to significantly reduce the morbidity and mortality associated with Diabetes. A method and apparatus for noninvasive measurement of blood glucose concentration based on transilluminated laser beam via the Index Finger has been reported in this paper. This method depends on photodiode based laser operating at 632.8 nm wavelength. During measurement, the index finger is inserted into the glucose sensing unit, the transilluminated optical signal is converted into an electrical signal, compared with the reference electrical signal, and the obtained difference signal is processed by signal processing unit which presents the results in the form of blood glucose concentration. This method would enable the monitoring blood glucose level of the diabetic patient continuously, safely and noninvasively.

  3. Filter paper blood spot enzyme linked immunoassay for adiponectin and application in the evaluation of determinants of child insulin sensitivity.

    Directory of Open Access Journals (Sweden)

    Richard M Martin

    Full Text Available BACKGROUND: Adiponectin is an adipocyte-derived hormone that acts as a marker of insulin sensitivity. Bloodspot sampling by fingerstick onto filter paper may increase the feasibility of large-scale studies of the determinants of insulin sensitivity. We first describe the validation of an enzyme-linked immunoassay (ELISA for quantifying adiponectin from dried blood spots and then demonstrate its application in a large trial (PROBIT. METHODS: We quantified adiponectin from 3-mm diameter discs (≈3 µL of blood punched from dried blood spots obtained from: i whole blood standards (validation; and ii PROBIT trial samples (application in which paediatricians collected blood spots from 13,879 children aged 11.5 years from 31 sites across Belarus. We examined the distribution of bloodspot adiponectin by demographic and anthropometric factors, fasting insulin and glucose. RESULTS: In the validation study, mean intra-assay coefficients of variation (n=162 were 15%, 13% and 10% for 'low' (6.78 µg/ml, 'medium' (18.18 µg/ml and 'high' (33.13 µg/ml internal quality control (IQC samples, respectively; the respective inter-assay values (n=40 were 23%, 21% and 14%. The correlation coefficient between 50 paired whole bloodspot versus plasma samples, collected simultaneously, was 0.87 (95% CI: 0.78 to 0.93. Recovery of known quantities of adiponectin (between 4.5 to 36 µg/ml was 100.3-133%. Bloodspot adiponectin was stable for at least 30 months at -80°C. In PROBIT, we successfully quantified fasting adiponectin from dried blood spots in 13,329 of 13,879 (96% children. Mean adiponectin (standard deviation concentrations were 17.34 µg/ml (7.54 in boys and 18.41 µg/ml (7.92 in girls and were inversely associated with body mass index, fat mass, triceps and subscapular skin-fold thickness, waist circumference, height and fasting glucose. CONCLUSIONS: Bloodspot ELISA is suitable for measuring adiponectin in very small volumes of blood collected on filter paper

  4. What Can Be Learned from Recent New Drug Applications? A Systematic Review of Drug Interaction Data for Drugs Approved by the US FDA in 2015.

    Science.gov (United States)

    Yu, Jingjing; Zhou, Zhu; Owens, Katie H; Ritchie, Tasha K; Ragueneau-Majlessi, Isabelle

    2017-01-01

    As a follow up to previous reviews, the aim of the present analysis was to systematically examine all drug metabolism, transport, pharmacokinetics (PK), and drug-drug interaction (DDI) data available in the 33 new drug applications (NDAs) approved by the Food and Drug Administration (FDA) in 2015, using the University of Washington Drug Interaction Database, and to highlight the significant findings. In vitro, a majority of the new molecular entities (NMEs) were found to be substrates or inhibitors/inducers of at least one drug metabolizing enzyme or transporter. In vivo, 95 clinical DDI studies displayed positive PK interactions, with an area under the curve (AUC) ratio ≥ 1.25 for inhibition or ≤ 0.8 for induction. When NMEs were considered as victim drugs, 21 NMEs had at least one positive clinical DDI, with three NMEs shown to be sensitive substrates of CYP3A (AUC ratio ≥ 5 when coadministered with strong inhibitors): cobimetinib, isavuconazole (the active metabolite of prodrug isavuconazonium sulfate), and ivabradine. As perpetrators, nine NMEs showed positive inhibition and three NMEs showed positive induction, with some of these interactions involving both enzymes and transporters. The most significant changes for inhibition and induction were observed with rolapitant, a moderate inhibitor of CYP2D6 and lumacaftor, a strong inducer of CYP3A. Physiologically based pharmacokinetics simulations and pharmacogenetics studies were used for six and eight NMEs, respectively, to inform dosing recommendations. The effects of hepatic or renal impairment on the drugs' PK were also evaluated to support drug administration in these specific populations.

  5. Utility of magneto-electropolished ternary nitinol alloys for blood contacting applications.

    Science.gov (United States)

    Pulletikurthi, Chandan; Munroe, Norman; Stewart, Danique; Haider, Waseem; Amruthaluri, Sushma; Rokicki, Ryszard; Dugrot, Manuel; Ramaswamy, Sharan

    2015-10-01

    The thrombogenicity of a biomaterial is mainly dependent on its surface characteristics, which dictates its interactions with blood. Surface properties such as composition, roughness wettability, surface free energy, and morphology will affect an implant material's hemocompatibility. Additionally, in the realm of metallic biomaterials, the specific composition of the alloy and its surface treatment are important factors that will affect the surface properties. The utility of magneto-electropolished (MEP) ternary Nitinol alloys, NiTiTa, and NiTiCr as blood contacting materials was investigated. The hemcompatibility of these alloys were compared to mechanically polished (MP) metallic biomaterial counterparts. In vitro thrombogenicity tests revealed significantly less platelet adherence on ternary MEP Nitinol, especially MEP NiTi10Ta as compared to the MP metals (p Nitinol alloys can potentially be utilized for blood-contacting devices where, complications resulting from thrombogenicity can be minimized.

  6. Blood sugar test - blood

    Science.gov (United States)

    ... blood glucose level ( hypoglycemia ) may be due to: Hypopituitarism (a pituitary gland disorder) Underactive thyroid gland or ... tonic-clonic seizure Glucagon blood test Glucagonoma Hyperthyroidism Hypopituitarism Hypothyroidism Insulinoma Low blood sugar Multiple endocrine neoplasia ( ...

  7. Assessment of erythrocyte aggregation in whole blood samples by light backscattering: clinical applications

    Science.gov (United States)

    Priezzhev, Alexander V.; Firsov, Nikolai N.; Vyshlova, Marina G.; Lademann, Juergen; Richter, Heike; Kiesewetter, Holger; Mueller, Gerhard J.

    1999-05-01

    We report on the results of a collaborative effort made in the field of optical diagnostics of whole blood samples to study the ability of red blood cells to aggregate in a Couette chamber. We studied a possibility to quantitatively measure this ability as a function of the physiological state of blood donors. The aggregometer designed by the Russian coauthors of this paper and described in their earlier publications (see e.g. Proc SPIE 1884, 2100, 2678, 2982) was extensively used in the experiments performed in the Rheumatology Institute in Moscow and in the Charite Clinic in Berlin. The following parameters were measured: two characteristic times of RBC aggregation and the average spontaneous aggregation rate in the state of stasis, the average hydrodynamic strength of all aggregates and that of the largest aggregates. Different algorithms of the remission signal processing for the quantitative evaluation of the above parameters were compared. Reproducible alterations of the parameters from their normal values were obtained for blood samples from individuals suffering auto-immune disease and diabetes. Statistical data is reported proving high efficiency of the technique for the diagnostics of rheological disorders. Basing on these data the quantitative criteria of the heaviness of hemorheological state of the patients are proposed that are important for choosing specific therapies for which the patient is minimally resistant.

  8. Monitoring of tissue optical properties using OCT: application for blood glucose analysis

    Science.gov (United States)

    Larin, Kirill V.; Eledrisi, Mohsen S.; Ashitkov, Taras V.; Motamedi, Massoud; Esenaliev, Rinat O.

    2002-07-01

    Noninvasive monitoring of tissue optical properties in real time could significantly improve diagnostics and management of various diseases. Recently we proposed to use high- resolution Optical Coherence Tomography (OCT) technique for measurement of tissue scattering coefficient at the depth of up to 1mm. Our pilot studies performed in vitro and in vivo demonstrated that measurement of tissue scattering with this technique can potentially be applied for noninvasive monitoring of blood glucose concentration. High resolution and coherent photon detection of the OCT technique allowed detection of glucose-induced changes in the scattering coefficient. In this paper we report results of in vivo studies performed in dog, New Zealand rabbits, and first human subjects. OCT system with the wavelength of 1300 nm was used in our experiments. OCT signal slope was measured and compared with actual blood glucose concentration. Bolus glucose injections and glucose clamping administrations were used in animal studies. OCT signals were recorded form human subjects during oral glucose tolerance test. Results obtained form both animal and human studies show good correlation between slope of the OCT signals and actual blood glucose concentration measured using standard glucometesr. Sensitivity and accuracy of blood glucose concentrations monitoring with the OCT is discussed. Obtained result suggest that OCT is a promising technique for noninvasive monitoring of tissue analytes including glucose.

  9. The Popularization and Application of Cold Storage Red Blood Cells or Whole Blood at -80 ℃ of the Rh(D) Negative Patients in Surgical Operation

    Institute of Scientific and Technical Information of China (English)

    余忠清; 胡丽华; 韩敏; 饶神宗; 罗成伟

    2002-01-01

    Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients re-ceived the transfusion of CSRBC or whole blood stored at -80 ℃ for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K+, Na+, BUN, Cr, urine protein (URPO), UOB, Hb,HCT, serum total protein, relative to hemolytic reaction and blood volume before and after transfu sion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative for urine protein and UOB, and the levels of BUN and Cr were normal (P>0. 05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K+ and blood Na+ was normal with the difference being not significant before and after operation (P> 0. 05). Plasma protein was decreased,but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at -80 ℃ could be safely infused to the Rh (D) negative patients without side effects during the surgical operation.

  10. Evaluation of blood-brain barrier-stealth nanocomposites for in situ glioblastoma theranostics applications

    Science.gov (United States)

    Su, Chia-Hao; Tsai, Ching-Yi; Tomanek, Boguslaw; Chen, Wei-Yu; Cheng, Fong-Yu

    2016-04-01

    The blood-brain barrier (BBB) is a physiological structure of the blood vessels in the brain. The BBB efficiently traps most therapeutic drugs in the blood vessels and stops them from entering the brain tissue, resulting in a decreased therapeutic efficiency. In this study, we developed BBB-stealth nanocomposites composed of iron oxide (Fe3O4) nanoparticles (NPs) as a safe nanocarrier for glioblastoma therapy. We showed the antitumor activity of Dox/alg-Fe3O4 NPs using in vitro and in vivo tests. We demonstrated that G23-alg-Fe3O4 NPs crossed the BBB and entered the brain. In situ glioblastoma tumor-bearing mice were used to successfully evaluate the antitumor activity of G23-Dox/alg-Fe3O4 NPs. Magnetic resonance imaging (MRI) and bioluminescence imaging (BLI) confirmed the BBB crossing. The BBB-stealth nanocomposites show great potential for a proof-of-concept clinical trial as a theranostics platform for human brain tumor therapy.The blood-brain barrier (BBB) is a physiological structure of the blood vessels in the brain. The BBB efficiently traps most therapeutic drugs in the blood vessels and stops them from entering the brain tissue, resulting in a decreased therapeutic efficiency. In this study, we developed BBB-stealth nanocomposites composed of iron oxide (Fe3O4) nanoparticles (NPs) as a safe nanocarrier for glioblastoma therapy. We showed the antitumor activity of Dox/alg-Fe3O4 NPs using in vitro and in vivo tests. We demonstrated that G23-alg-Fe3O4 NPs crossed the BBB and entered the brain. In situ glioblastoma tumor-bearing mice were used to successfully evaluate the antitumor activity of G23-Dox/alg-Fe3O4 NPs. Magnetic resonance imaging (MRI) and bioluminescence imaging (BLI) confirmed the BBB crossing. The BBB-stealth nanocomposites show great potential for a proof-of-concept clinical trial as a theranostics platform for human brain tumor therapy. Electronic supplementary information (ESI) available: Experimental details. See DOI: 10.1039/c6nr00280c

  11. Application of psychological consultation technique in blood donation%浅谈心理咨询技能在无偿献血工作中的应用

    Institute of Scientific and Technical Information of China (English)

    冯刚丽

    2014-01-01

    Blood donors have various degrees of fears which produce an effect on their blood donation. The application of psychological consultation technique can help blood donors set up their blood donation concept. It’s vital to ensure the blood safety by effecting psychological nursing for blood donors, providing excellent blood collection service and developing more blood donors with low risk. This paper simply states the application of psychological consultation technique in blood donation.%在无偿献血的工作中,献血者有着不同程度的恐惧心理,严重影响献血行为。因此,运用心理咨询技术,帮助献血者树立正确的献血观念。对无偿献血者实施有效的心理护理,提供优质的采血服务,发展低危无偿献血者。不断建立和扩大自愿无偿献血者队伍,对保证血液安全至关重要。本文就心理咨询技能在无偿献血中的应用体会作简单地阐述。

  12. Waste Feed Delivery Environmental Permits and Approvals Plan

    Energy Technology Data Exchange (ETDEWEB)

    TOLLEFSON, K.S.

    2000-01-18

    This plan describes the environmental permits approvals, and other requirements that may affect establishment of a waste feed delivery system for the Hanford Site's River Protection Project. This plan identifies and screens environmental standards for potential applicability, outlines alternatives for satisfying applicable standards, and describes preferred permitting and approval approaches.

  13. The study of the Golgi apparatus in blood--basic science and clinical applications.

    Science.gov (United States)

    Zeng, Liuwang; Hu, Zhiping; Lu, Wei; Tang, Xiangqi; Zhang, Jie; Li, Ting; Yang, Binbin

    2010-01-01

    The Golgi apparatus (GA) is a cytoplasmic organelle that is of great interest to all scientists for its key role in the biosynthesis, transporting and sorting of both lipids and proteins located at the intersection of the secretory and endocytic pathways. Recently, more and more evidence shows that changes in the Golgi apparatus play an important role in the clinical progression and pathological development of many diseases. In this review, we will summarize the alteration of the Golgi apparatus in blood cells and anti-Golgi complex antibodies in blood serum under different conditions and further clarify the contribution of the Golgi apparatus dysfunction to the course of these diseases and its pathophysiological basis, which will significantly improve our understanding and impact our ability to develop more effective therapies for these diseases.

  14. The 'new genetics' in blood and cardiovascular research: applications to prevention and treatment.

    Science.gov (United States)

    Motulsky, A G

    1984-11-01

    Genetic approaches have become an important component of both fundamental and disease-oriented research. Certain diseases of the blood--the hemoglobinopathies--have been elucidated by the spectacular methods of molecular genetics. Some of these advances have already been incorporated in disease management. Often, common conditions such as coronary heart disease and hypertension show familial aggregation. Genetic analysis of these diseases together with biochemical and molecular methods are likely to be useful for further understanding and ultimate prevention and control.

  15. Clinical application of whole blood red cell distribution width in lung cancer metastasis

    Institute of Scientific and Technical Information of China (English)

    徐阳飏

    2014-01-01

    Objective To investigate the clinical value of whole blood red cell distribution width(RDW)in discriminating lung cancer metastasis.Methods A retropective analysis was conducted on the patients who were initially diagnosed as primary lung cancer.A total of 525 patients were included for analysis between January 2012 and July2013,stratified by different stages and metastasis scenarios.RDW data was investigated.Kruskal-Wallis H tests

  16. A high-throughput assay of NK cell activity in whole blood and its clinical application

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Saet-byul [Department of Microbiology and Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul (Korea, Republic of); Cha, Junhoe [ATGen Co. Ltd., Sungnam (Korea, Republic of); Kim, Im-kyung [Department of Surgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul (Korea, Republic of); Yoon, Joo Chun [Department of Microbiology, Ewha Womans University School of Medicine, Seoul (Korea, Republic of); Lee, Hyo Joon [Department of Microbiology and Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul (Korea, Republic of); Park, Sang Woo; Cho, Sunjung; Youn, Dong-Ye; Lee, Heyja; Lee, Choong Hwan [ATGen Co. Ltd., Sungnam (Korea, Republic of); Lee, Jae Myun [Department of Microbiology and Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul (Korea, Republic of); Lee, Kang Young, E-mail: kylee117@yuhs.ac [Department of Surgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul (Korea, Republic of); Kim, Jongsun, E-mail: jkim63@yuhs.ac [Department of Microbiology and Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul (Korea, Republic of)

    2014-03-14

    Graphical abstract: - Highlights: • We demonstrated a simple assay of NK cell activity from whole blood. • The measurement of secreted IFN-γ from NK cell enables high-throughput screening. • The NKA assay was validated by clinical results of colorectal cancer patients. - Abstract: Natural killer (NK) cells are lymphocytes of the innate immune system and have the ability to kill tumor cells and virus-infected cells without prior sensitization. Malignant tumors and viruses have developed, however, strategies to suppress NK cells to escape from their responses. Thus, the evaluation of NK cell activity (NKA) could be invaluable to estimate the status and the outcome of cancers, viral infections, and immune-mediated diseases. Established methods that measure NKA, such as {sup 51}Cr release assay and CD107a degranulation assay, may be used to determine NK cell function, but they are complicated and time-consuming because they require isolation of peripheral blood mononuclear cells (PBMC) or NK cells. In some cases these assays require hazardous material such as radioactive isotopes. To overcome these difficulties, we developed a simple assay that uses whole blood instead of PBMC or isolated NK cells. This novel assay is suitable for high-throughput screening and the monitoring of diseases, because it employs serum of ex vivo stimulated whole blood to detect interferon (IFN)-γ secreted from NK cells as an indicator of NKA. After the stimulation of NK cells, the determination of IFNγ concentration in serum samples by enzyme-linked immunosorbent assay (ELISA) provided a swift, uncomplicated, and high-throughput assay of NKA ex vivo. The NKA results microsatellite stable (MSS) colorectal cancer patients was showed significantly lower NKA, 263.6 ± 54.5 pg/mL compared with healthy subjects, 867.5 ± 50.2 pg/mL (p value <0.0001). Therefore, the NKA could be utilized as a supportive diagnostic marker for microsatellite stable (MSS) colorectal cancer.

  17. Blood Flow Clustering and Applications in Virtual Stenting of Intracranial Aneurysms.

    Science.gov (United States)

    Oeltze, Steffen; Lehmann, Dirk J; Kuhn, Alexander; Janiga, Gabor; Theisel, Holger; Preim, Bernhard

    2014-05-01

    Understanding the hemodynamics of blood flow in vascular pathologies such as intracranial aneurysms is essential for both their diagnosis and treatment. Computational fluid dynamics (CFD) simulations of blood flow based on patient-individual data are performed to better understand aneurysm initiation and progression and more recently, for predicting treatment success. In virtual stenting, a flow-diverting mesh tube (stent) is modeled inside the reconstructed vasculature and integrated in the simulation. We focus on steady-state simulation and the resulting complex multiparameter data. The blood flow pattern captured therein is assumed to be related to the success of stenting. It is often visualized by a dense and cluttered set of streamlines.We present a fully automatic approach for reducing visual clutter and exposing characteristic flow structures by clustering streamlines and computing cluster representatives. While individual clustering techniques have been applied before to streamlines in 3D flow fields, we contribute a general quantitative and a domain-specific qualitative evaluation of three state-of-the-art techniques. We show that clustering based on streamline geometry as well as on domain-specific streamline attributes contributes to comparing and evaluating different virtual stenting strategies. With our work, we aim at supporting CFD engineers and interventional neuroradiologists.

  18. Novel single-cell functional analysis of red blood cells using laser tweezers Raman spectroscopy: application for sickle cell disease.

    Science.gov (United States)

    Liu, Rui; Mao, Ziliang; Matthews, Dennis L; Li, Chin-Shang; Chan, James W; Satake, Noriko

    2013-07-01

    Laser tweezers Raman spectroscopy was used to characterize the oxygenation response of single normal adult, sickle, and cord blood red blood cells (RBCs) to an applied mechanical force. Individual cells were subjected to different forces by varying the laser power of a single-beam optical trap, and the intensities of several oxygenation-specific Raman spectral peaks were monitored to determine the oxygenation state of the cells. For all three cell types, an increase in laser power (or mechanical force) induced a greater deoxygenation of the cell. However, sickle RBCs deoxygenated more readily than normal RBCs when subjected to the same optical forces. Conversely, cord blood RBCs were able to maintain their oxygenation better than normal RBCs. These results suggest that differences in the chemical or mechanical properties of fetal, normal, and sickle cells affect the degree to which applied mechanical forces can deoxygenate the cell. Populations of normal, sickle, and cord RBCs were identified and discriminated based on this mechanochemical phenomenon. This study demonstrates the potential application of laser tweezers Raman spectroscopy as a single-cell, label-free analytical tool to characterize the functional (e.g., mechanical deformability, oxygen binding) properties of normal and diseased RBCs.

  19. Drugs Approved for Thyroid Cancer

    Science.gov (United States)

    ... Ask about Your Treatment Research Drugs Approved for Thyroid Cancer This page lists cancer drugs approved by the ... that are not listed here. Drugs Approved for Thyroid Cancer Cabozantinib-S-Malate Caprelsa (Vandetanib) Cometriq (Cabozantinib-S-Malate) Doxorubicin ...

  20. Drugs Approved for Lung Cancer

    Science.gov (United States)

    ... Ask about Your Treatment Research Drugs Approved for Lung Cancer This page lists cancer drugs approved by the ... listed here. Drugs Approved for Non-Small Cell Lung Cancer Abitrexate (Methotrexate) Abraxane (Paclitaxel Albumin-stabilized Nanoparticle Formulation) ...

  1. Application of DSA and ultrasonic blood rheography (Doppler) to benign paroxysmal positional vertigo

    Energy Technology Data Exchange (ETDEWEB)

    Fujita, Nobuya; Wada, Yoshiro; Suzumura, Shigeo; Matsunaga, Takashi (Nara Medical Univ., Kashihara (Japan))

    1990-03-01

    Fourteen cases of benign paroxysmal positional vertigo (BPPV) were studied with cervical circulation using DSA and Doppler. DSA study showed 7 abnormal cases out of 10 cases of BPPV. The abnormal findings included 5 cases of stenosis and 5 cases of coiling and kinking in cervical vertebral arteries. Doppler study showed that vertebral a. and also carotid a. had low volume and high laterality of cervical blood circulation in parameter of flow and velocity. These findings suggest the cervical circulation disorder was one of background factors in pathogenesis of BPPV. (author).

  2. Dual stack black blood carotid artery CMR at 3T: Application to wall thickness visualization

    Directory of Open Access Journals (Sweden)

    Marx Nikolaus

    2009-11-01

    Full Text Available Abstract Background The increasing understanding of atherosclerosis as an important risk factor for the development of acute ischemic events like ischemic stroke has stimulated increasing interest in non-invasive assessment of the structure, composition and burden of plaque depositions in the carotid artery wall. Vessel wall imaging by means of cardiovascular magnetic resonance (CMR is conventionally done by 2D dual inversion recovery (DIR techniques, which often fail in covering large volumes of interest as required in plaque burden assessment. Although the technique has been extended to 2D multislice imaging, its straight extension to 3D protocols is still limited by the prolonged acquisition times and incomplete blood suppression. A novel approach for rapid overview imaging of large sections of the carotid artery wall at isotropic spatial resolutions is presented, which omits excitation of the epiglottis. By the interleaved acquisition of two 3D stacks with the proposed motion sensitized segmented steady-state black-blood gradient echo technique (MSDS the coverage of the carotid artery trees on both sides in reasonable scan times is enabled. Results 10 patients were investigated with the proposed technique and compared to conventional transversal DIR turbo spin and gradient echo approaches centered at the height of the carotid bifurcation. In all MSDS experiments sufficient black-blood contrast could be obtained over the entire covered volumes. The contrast to noise ratio between vessel and suppressed blood was improved by 73% applying the motion sensitizing technique. In all patients the suspicious areas of vessel wall thickening could be clearly identified and validated by the conventional local imaging approach. The average assessable vessel wall segment length was evaluated to be 18 cm. While in 50% of the cases motion artifacts could be appreciated in the conventional images, none were detected for the MSDS technique. Conclusion The

  3. Alexander Fleming, citrated blood and penicillin: paths not pursued and applications delayed.

    Science.gov (United States)

    Mortimer, P P

    2009-12-01

    Ninety years ago Alexander Fleming (later to discover penicillin) jointly wrote a description of the use of indirect transfusions of citrated blood at a World War 1 (WW1) base hospital. It was the longest series yet to be published, incorporating what was then a novel procedure for treating war casualties. Returning to civilian life Fleming, a qualified surgeon and bacteriologist, chose a different career path, and not until the wars of the late 1930s were the advances in transfusion in WW1 fully incorporated into the management of trauma and haemorrhage. Like penicillin, the benefits of indirect transfusion were only slowly realised.

  4. Comparable application of the OCT and Abbe refractometers for measurements of glycated hemoglobin portion in blood

    Science.gov (United States)

    Zhernovaya, Olga S.; Tuchin, Valery V.; Wang, Ruikang K.

    2006-02-01

    It is known that glucose interacts with plasma proteins and hemoglobin in erythrocytes. Glycated (glycosylated) hemoglobin is the result of an irreversible non-enzymatic fixation of glucose on the beta chain of hemoglobin A. The amount of glycated hemoglobin depends on blood glucose concentration and reflects the mean glycemia of about the previous 2-3 months. Glycated hemoglobin is a useful marker for long-term glucose control in diabetic patients. Therefore, the search of quick and high sensitive methods for measurement of glycated hemoglobin portion in blood is important. This study is focused on the determination of refractive index of hemoglobin solution at different glucose concentrations. Measurements were performed using Abbe refractometer at 589 nm and optical coherence tomography (OCT) at 820 nm. The different amount of glucose (from 0 to 1000 mg/dl with a step 100 mg/dl) was added to hemoglobin solution. Theoretical values of refractive index of hemoglobin solutions with glucose were calculated supposing non-interacting hemoglobin and glucose molecules. There is a difference between measured and calculated values of refractive index. This difference is due to glucose binding to hemoglobin. It is shown that the refractive index measurements can be applied for the evaluation of glycated hemoglobin amount.

  5. Fully Autonomous Multiplet Event Detection: Application to Local-Distance Monitoring of Blood Falls Seismicity

    Energy Technology Data Exchange (ETDEWEB)

    Carmichael, Joshua Daniel [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Carr, Christina [Univ. of Alaska, Fairbanks, AK (United States); Pettit, Erin C. [Univ. of Alaska, Fairbanks, AK (United States)

    2015-06-18

    We apply a fully autonomous icequake detection methodology to a single day of high-sample rate (200 Hz) seismic network data recorded from the terminus of Taylor Glacier, ANT that temporally coincided with a brine release episode near Blood Falls (May 13, 2014). We demonstrate a statistically validated procedure to assemble waveforms triggered by icequakes into populations of clusters linked by intra-event waveform similarity. Our processing methodology implements a noise-adaptive power detector coupled with a complete-linkage clustering algorithm and noise-adaptive correlation detector. This detector-chain reveals a population of 20 multiplet sequences that includes ~150 icequakes and produces zero false alarms on the concurrent, diurnally variable noise. Our results are very promising for identifying changes in background seismicity associated with the presence or absence of brine release episodes. We thereby suggest that our methodology could be applied to longer time periods to establish a brine-release monitoring program for Blood Falls that is based on icequake detections.

  6. Fluctuations of coupled fluid and solid membranes with application to red blood cells

    Science.gov (United States)

    Auth, Thorsten; Safran, S. A.; Gov, Nir S.

    2007-11-01

    The fluctuation spectra and the intermembrane interaction of two membranes at a fixed average distance are investigated. Each membrane can either be a fluid or a solid membrane, and in isolation, its fluctuations are described by a bare or a wave-vector-dependent bending modulus, respectively. The membranes interact via their excluded-volume interaction; the average distance is maintained by an external, homogeneous pressure. For strong coupling, the fluctuations can be described by a single, effective membrane that combines the elastic properties. For weak coupling, the fluctuations of the individual, noninteracting membranes are recovered. The case of a composite membrane consisting of one fluid and one solid membrane can serve as a microscopic model for the plasma membrane and cytoskeleton of the red blood cell. We find that, despite the complex microstructure of bilayers and cytoskeletons in a real cell, the fluctuations with wavelengths λ≳400nm are well described by the fluctuations of a single, polymerized membrane (provided that there are no inhomogeneities of the microstructure). The model is applied to the fluctuation data of discocytes (“normal” red blood cells), a stomatocyte, and an echinocyte. The elastic parameters of the membrane and an effective temperature that quantifies active, metabolically driven fluctuations are extracted from the experiments.

  7. The hydrogen gas clearance method for liver blood flow examination: inhalation or local application of hydrogen?

    Science.gov (United States)

    Metzger, H P

    1989-01-01

    The combined method of hydrogen inhalation and local hydrogen production enable the determination of hepatic blood flow (HBF) and local hepatic blood flow (LHBF). LHBF was registered within a small superficial tissue volume of 0.5 mm in diameter by means of a multi-wire electrode having 200 microns producing and 100 microns measuring wires arranged within less than 300 microns distance between the measuring wires. The feeding current for hydrogen production was 1 microA, the potential less than 10 V. The clearance in response to inhalation was registered by means of the same measuring electrodes within the same tissue volume. Spontaneously breathing rats (Wistar-Frömter strain, 180-230 g bw, N = 19, ketamin-xylazine anesthesia, artificial respiration) showed the following flow values: HBF +/- SD = 0.50 +/- 0.26 ml/g.min, n = 48 registrations; LHBF +/- SD = 4.66 +/- 2.13 ml/g.min, n = 43. The validity of the combined method is demonstrated in the LHBF/HBF graph which summarizes the data of hemorrhagic and control animals, m = 0.1 and yo = 0.001. The correlation coefficient of r = 0.685 shows a reasonable correlation of the combined data despite the wide scattering of the individual values.

  8. Transport of nanoparticles through the blood-brain barrier for imaging and therapeutic applications

    Science.gov (United States)

    Shilo, Malka; Motiei, Menachem; Hana, Panet; Popovtzer, Rachela

    2014-01-01

    A critical problem in the treatment of neurodegenerative disorders and diseases, such as Alzheimer's and Parkinson's, is the incapability to overcome the restrictive mechanism of the blood-brain barrier (BBB) and to deliver important therapeutic agents to the brain. During the last decade, nanoparticles have gained attention as promising drug delivery agents that can transport across the BBB and increase the uptake of appropriate drugs in the brain. In this study we have developed insulin-targeted gold nanoparticles (INS-GNPs) and investigated quantitatively the amount of INS-GNPs that cross the BBB by the receptor-mediated endocytosis process. For this purpose, INS-GNPs and control GNPs were injected into the tail vein of male BALB/c mice. Major organs were then extracted and a blood sample was taken from the mice, and thereafter analyzed for gold content by flame atomic absorption spectroscopy. Results show that two hours post-intravenous injection, the amount of INS-GNPs found in mouse brains is over 5 times greater than that of the control, untargeted GNPs. Results of further experimentation on a rat model show that INS-GNPs can also serve as CT contrast agents to highlight specific brain regions in which they accumulate. Due to the fact that they can overcome the restrictive mechanism of the BBB, this approach could be a potentially valuable tool, helping to confront the great challenge of delivering important imaging and therapeutic agents to the brain for detection and treatment of neurodegenerative disorders and diseases.

  9. 21 CFR 640.6 - Modifications of Whole Blood.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Modifications of Whole Blood. 640.6 Section 640.6...) BIOLOGICS ADDITIONAL STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Whole Blood § 640.6 Modifications of Whole Blood. Upon approval by the Director, Center for Biologics Evaluation and Research, of a supplement...

  10. A REVIEW ON EFFECTIVE APPLICATION OF AN ENAMEL MATRIX DERIVATIVE (EMDOGAIN® FOR PERIODONTAL SURGERY IN THE PRESENCE OF BLOOD

    Directory of Open Access Journals (Sweden)

    Oana M. CALUSERU

    2013-06-01

    Full Text Available Reconstructive periodontal surgery aims at predictably restoring tooth’s supporting structure lost due to perio‐ dontal disease or trauma. One such modality, which has been demonstrate to promote periodontal regeneration, is an enamel matrix derivative (EMD, consisting of a formu‐ lation of amelogenin proteins from developing porcine enamel. This review article provides a brief update on the effects of blood interaction, occurring during periodontal surgery, on the effectiveness of EMD adsorption on the root surfaces and its implications for periodontal recon‐ structive surgery.The clinical use of an enamel matrix derivative (EMD has been successfully proved in periodontal surgery, as promoting regeneration of periodontal tissues including new cementum, periodontal ligament (PDL and alveolar bone [1]. Despite its widespread use, only recently has the effect of blood, occurring during periodontal surgery, been evaluated for contamination of the effectiveness of EMD adsorption onto root surfaces. The aim of this review arti‐ cle is to provide the clinician a summary of findings from in vitro experiments testing the effects of EMD adsorption onto root surfaces in the presence and absence of blood and its effect on PDL cell behavior [2]. Until recently, the extent to which bleeding occurring during periodontal sur‐ gery affects the adsorption of EMD onto root surfaces could not be established.In summary, the teeth extracted for orthodontic reasons were subject to ex vivo scaling and root planing and expo‐ sed to 6 clinically relevant scenarios, as illustrated in figure 1. EMD application is usually performed following root surface conditioning with 24% EDTA. Findings from high resolution scanning electron microscopy (SEM demons‐ trated that the proteins found in blood (mainly albumin were able to compete with those found in EMD (Figure 2, reducing the effectiveness of EMD thereafter. No apparent effect of conditioning the surface

  11. Application of a modified regularization procedure for estimating oxygen tension in large retinal blood vessels

    Science.gov (United States)

    Yildirim, Isa; Ansari, Rashid; Samil Yetik, I.; Shahidi, Mahnaz

    2010-03-01

    Phosphorescence lifetime measurement based on a frequency domain approach is used to estimate oxygen tension in large retinal blood vessels. The classical least squares (LS) estimation was initially used to determine oxygen tension indirectly from intermediate variables. A spatial regularized least squares (RLS) method was later proposed to reduce the high variance of oxygen tension estimated by LS method. In this paper, we provide a solution using a modified RLS (MRLS) approach that utilizes prior knowledge about retinal vessels oxygenation based on expected oxygen tension values in retinal arteries and veins. The performance of MRLS method was evaluated in simulated and experimental data by determining the bias, variance, and mean absolute error (MAE) of oxygen tension measurements and comparing these parameters with those derived with the use of LS and RLS methods.

  12. Application of Ambulatory Blood Pressure Monitoring Essential Hypertension with Target-Organ Damage

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@The purpose of this study was to determine whether this normal ambulatory blood pressure (ABP) criterion deter-mined by JVC-VI was suitable for Chinese patients with es-sential hypertention (EH), in order to decrease target organ damage (TOD). 24-hour ABP monitoring (ABPM) results in 1 325 cases with reliable data according with the selected criteria were investigated. 106 normotensives (65 men, 41women, mean age 52 years), 498 untreated simple hyper-tensives (288 men, 210 women, mean age 54 years) and 722 recently untreated hypertensives with TOD (490 men,232 women, mean age 58 years) including 53% cardiac damage, 16% cerebral damage, 9% renal damage and 22 % more than one organ damage were studied.

  13. First application of comet assay in blood cells of Mediterranean loggerhead sea turtle (Caretta caretta).

    Science.gov (United States)

    Caliani, Ilaria; Campani, Tommaso; Giannetti, Matteo; Marsili, Letizia; Casini, Silvia; Fossi, Maria Cristina

    2014-05-01

    The aim of this study was to validate the comet assay in erythrocytes of Caretta caretta, a species never investigated for genotoxicity. We studied 31 loggerhead sea turtles from three Italian marine rescue centres. Peripheral blood samples were collected from all the animals and the comet assay applied. All comet cells were analysed using two methods: visual scoring and computer image analysis. The % DNA in tail mean value ± SD and Damage Index were 21.56 ± 15.41 and 134.83 ± 94.12, respectively. A strong and statistically significant statistically correlation between the two analytical methods was observed (r = 0.95; p comet assay is a useful method to detect the possible effects of genotoxic agents in loggerhead sea turtle and to increase the knowledge about the ecotoxicological health status of this threatened species.

  14. Total 3D imaging of phase objects using defocusing microscopy: application to red blood cells

    CERN Document Server

    Roma, P M S; Amaral, F T; Agero, U; Mesquita, O N

    2014-01-01

    We present Defocusing Microscopy (DM), a bright-field optical microscopy technique able to perform total 3D imaging of transparent objects. By total 3D imaging we mean the determination of the actual shapes of the upper and lower surfaces of a phase object. We propose a new methodology using DM and apply it to red blood cells subject to different osmolality conditions: hypotonic, isotonic and hypertonic solutions. For each situation the shape of the upper and lower cell surface-membranes (lipid bilayer/cytoskeleton) are completely recovered, displaying the deformation of RBCs surfaces due to adhesion on the glass-substrate. The axial resolution of our technique allowed us to image surface-membranes separated by distances as small as 300 nm. Finally, we determine volume, superficial area, sphericity index and RBCs refractive index for each osmotic condition.

  15. Clinical application of disposable heparin sensors. Blood heparin measurements during open heart surgery.

    Science.gov (United States)

    Yun, J H; Lee, L M; Wahr, J A; Fu, B; Meyerhoff, M E; Yang, V C

    1995-01-01

    The authors previously reported the development of an ion selective electrode type heparin sensor consisting of a specially formulated polymer membrane doped with tridodecylmethylammonium chloride as the heparin complexing agent. They also demonstrated the feasibility of measuring blood heparin levels by protamine titration, using a disposable copper wire sensor coated with the heparin sensing membrane to probe the titration end point. In this article, the results of further titration studies conducted on 44 clinical whole blood specimens obtained from 8 patients undergoing open heart surgery were reviewed. Samples were taken from patients at four different stages during the bypass surgery: 1) before heparin administration; 2) immediately after heparin administration; 3) within 30 min to 3 hr after heparin administration; and 4) within 30 min after protamine administration. Heparin anticoagulant activity in these samples was monitored by the activated clotting time assay, whereas heparin concentrations were measured by protamine titration using either the Hepcon HMS Titrator (Medtronic HemoTec Inc., Englewood, CO) or the coated wire heparin sensor to determine titration end points. Results indicate that heparin levels determined by the sensor method were in good agreement with those determined by the Hepcon HMS Titrator. When the heparin concentrations estimated by the two methods show significant discrepancy (> 1.0 unit/ml), the sensor method seems to provide more precise values, as verified by an additional chromogenic heparin assay. The overall time required to complete the titration process and heparin measurement with a pre made heparin sensor was less than 3 min. Clinically, the heparin sensor could be used as a safeguard to precisely monitor heparin levels during surgical procedures. Alternatively, the sensor could be used to assess the accurate protamine dose required for full heparin reversal.

  16. Quantification of cerebral blood flow and its clinical usefulness. Application of SPECT to psychiatry

    Energy Technology Data Exchange (ETDEWEB)

    Matsuda, Hiroshi; Uema, Takeshi; Kogure, Daisuke; Takano, Harumasa; Terada, Tomo [National Center Hospital for Mental, Nervous and Muscular Disorders, Kodaira, Tokyo (Japan)

    1998-10-01

    Brain perfusion SPECT using {sup 99m}Tc-ethyl-cysteinate dimer ({sup 99m}Tc-ECD) was applied to psychiatric diseases with aid of statistical parametric mapping (SPM) for analysis of data. To evaluate influence of aging on brain perfusion, noninvasive measurements of cerebral blood flow using {sup 99m}Tc-ECD were performed in 53 normal volunteers, aged 18 to 87 years old. Mean cerebral blood flow (mCBF) was 43.9{+-}5.0 ml/100 g/min and showed weak negative correlation with aging (r=-0.451). Perisylvian cerebral cortices and medial frontal areas including anterior cingulate gyri showed greater negative correlation than other areas. These findings suggest the necessity of age-matched control regional CBF (rCBF) data to investigate rCBF abnormality in patients. Four drug-naive schizophrenic patients showed flow decrease in bilateral frontal and superior temporal areas and a left infero-posterior temporal area. Haloperidol administration induced flow decrease in bilateral frontal and left parietal areas, while flow increase in bilateral striatal and right hippocampal areas. Ten aged depressive patients showed flow decrease in bilateral frontal and left temporo-parietal areas. Even after remission patients showed flow decrease in the left frontal area as compared with normal subjects. Remission induced flow increase in the right frontal, right parietal, and right orbitofrontal areas compared with depression. These results suggest that CBF measurements using {sup 99m}Tc-ECD are useful for objective evaluation of regional abnormality in brain function in psychiatric diseases. (author)

  17. 14 CFR 21.333 - Issue of export airworthiness approval tags for Class III products.

    Science.gov (United States)

    2010-01-01

    ... for Class III products. 21.333 Section 21.333 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Approvals § 21.333 Issue of export airworthiness approval tags for Class III products. (a) An applicant is entitled to an export airworthiness approval tag for Class III products if that applicant shows, except...

  18. Drugs Approved for Retinoblastoma

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for retinoblastoma. The list includes generic names and brand names. The drug names link to NCI’s Cancer Drug Information summaries.

  19. Drugs Approved for Neuroblastoma

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for neuroblastoma. The list includes generic names and brand names. The drug names link to NCI's Cancer Drug Information summaries.

  20. FWS Approved Acquisition Boundaries

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This data layer depicts the external boundaries of lands and waters that are approved for acquisition by the U.S. Fish and Wildlife Service (USFWS) in North America,...

  1. Premarket Approvals (PMA)

    Data.gov (United States)

    U.S. Department of Health & Human Services — Premarket approval by FDA is the required process of scientific review to ensure the safety and effectiveness of all devices classified as Class III devices. An...

  2. FWS Approved Acquisition Boundaries

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This data layer depicts the external boundaries of lands and waters that are approved for acquisition by the U.S. Fish and Wildlife Service (USFWS) in North...

  3. 75 FR 80493 - Public Water System Supervision Program Approval for the State of Wisconsin

    Science.gov (United States)

    2010-12-22

    ... From the Federal Register Online via the Government Publishing Office ENVIRONMENTAL PROTECTION AGENCY Public Water System Supervision Program Approval for the State of Wisconsin AGENCY: Environmental... of Wisconsin submitted a primacy application for its approved Public Water System Supervision...

  4. Analysis of the clinical application of irradiated blood components%分析辐照血液成分的临床应用

    Institute of Scientific and Technical Information of China (English)

    袁晶; 张跃进

    2016-01-01

    Objective To explore the blood center blood irradiation of various blood components in clinical application. Methods a retrospective 2014 (control group) and 2015 (experimental group) two years the blood center of irradiated blood for blood component used in clinical treatment, irradiation of blood components in clinical usage and compare whether there are differences in different years.Results in the experimental group, the 2015 group compared to the control group in the year 2014 group, the amount of irradiated blood accounted for the total blood volume increased significantly, also irradiated blood components such as red blood cell components, platelet components more application in clinical, the difference is statistically significant (P<0.05Conclusion the application of irradiated blood components in clinical, does not affect the quality of blood transfusion and reduce the incidence of transfusion associated graft versus host disease, and can be widely used in clinic.%目的:探究本血液中心辐照血的血液各种成分在临床的应用情况。方法回顾2014年9月至次年三月(对照组)与2015年4月至今(实验组)两年本血液中心辐照血的血液成分应用于临床治疗的情况,研究辐照血液成分在临床的使用率并比较不同年限是否有差异。结果实验组即2015年组相比于对照组即2014年组,辐照血用量占总用血量明显增多,同时辐照血液成分如红细胞血液成分、血小板成分更多的应用于临床,差异有统计学意义(P<0.05)。结论辐照血液成分应用于临床,不影响输血质量的同时减少了输血相关性移植物抗宿主病的发生情况,临床可以广泛应用。

  5. 路径管理在血站体采科中的应用探讨%The application of the administer of pathway in the Physical examination & blood collection department of the Blood-bank

    Institute of Scientific and Technical Information of China (English)

    陈秀兰

    2012-01-01

    Objective To investigate the effcet on of the application of the administer of pathway in the Physical examination & blood collection department of the Blood-bank. Methods The 400 blood donors were probabilistically separated for the control group and the experimental group, based on 200 cases each group. The analysis to evaluate was proceed by the degree of the blood donors satisfaction and the incidence rate of the blood donation reaction. Results The degree of the blood donors satisfaction: The total degrees of the blood donors satisfaction for the control group and the experimental group are 94.5% and 99.5%, and the degree of the blood donors satisfaction for the experimental group was obviously improved. The results of both the degrees of the blood donors satisfaction were compared, and their differences all were statistically significant (p<0.01). The incidence rate of the blood donation reaction: The incidence rates of the blood donation reaction for the control group and the experimental group are 4.5% and 1%, and the incidence rate of the blood donation reaction for the experimental group was obviously reduced. The results of both t the incidence rates of the blood donation reaction were compared, and their differences all were statistically significant (p<0.05). Conclusions It is ideal to repair the soft tissue defect of the thumb’s fingertips, and there is a high survival rate and a good sensory function recovery, which was worth selecting and applying reasonably, basing on environment concerned therapy. The administer of the blood collection pathway to carry out could improve the whole service function of the the Physical examination & blood collection department of the Blood-bank, and the social benefit and the quality of the job for the Blood-bank, which was worth to extend.%目的探讨路径管理在血站体采科应用的影响.方法将400例献血者随机分为对照组和实验组,每组200例,对两组献血者的满意度和献血反

  6. Application of a blood-brain-barrier-penetrating form of GDNF in a mouse model for Parkinson's disease.

    Science.gov (United States)

    Dietz, Gunnar P H; Valbuena, Paoloa C; Dietz, Birgit; Meuer, Katrin; Müeller, Patrick; Weishaupt, Jachen H; Bähr, Mathias

    2006-04-12

    Glial-cell-line-derived neurotrophic factor (GDNF) promotes mesencephalic dopaminergic neuronal survival in several in vitro and in vivo models. As the demise of dopaminergic neurons is the cause for Parkinson's disease (PD) symptoms, GDNF is a promising agent for its treatment. However, this neurotrophin is unable to cross the blood-brain barrier, which has complicated its clinical use. Therefore, ways to deliver GDNF into the central nervous system in an effective manner are needed. The HIV-1-Tat-derived cell-penetrating peptide (CPP) provides a means to deliver fusion proteins into the brain. We generated a fusion protein between the 11 amino acid CPP of Tat and the rat GDNF mature protein to deliver GDNF across the blood-brain barrier. We showed previously that Tat-GDNF enhances the neuroprotective effect of GDNF in in vivo models for nerve trauma and ischemia. Here, we tested its effect in a subchronic scheme of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) application into the mouse as a model for PD to evaluate the effect of Tat-GDNF fusion protein in dopaminergic neuron survival. We showed that the fusion protein did indeed reach the dopaminergic neurons. However, the in vivo application of Tat-GDNF did not provide neuroprotection of dopaminergic neurons, as revealed by immunohistochemistry and counting of the number of tyrosine-hydroxylase-immunoreactive neurons in the substantia nigra pars compacta. Possibly, GDNF does protect nigro-striatal projections of those neurons that survive MPTP treatment but does not increase the number of surviving dopaminergic neurons. A concomitant treatment of Tat-GDNF with an anti-apoptotic Tat-fusion protein might be beneficial.

  7. Non-invasive blood pressure measurement: values, problems and applicability in the common marmoset (Callithrix jacchus).

    Science.gov (United States)

    Mietsch, M; Einspanier, A

    2015-07-01

    The common marmoset (Callithrix jacchus, C. j.) is an established primate model in biomedical research and for human-related diseases. Monitoring of cardiovascular parameters including blood pressure (BP) is important for the health surveillance of these experimental animals and the quantification of diseases or pharmaceutical substances influencing BP. Measurement guidelines for C. j. do not exist yet; therefore, the present study was carried out to establish a practicable protocol based on recommendations of the American College of Veterinary Internal Medicine (ACVIM). Furthermore, BP data of 49 marmosets (13.8-202.4 months of age) were obtained via high-definition oscillometry to further knowledge of physiological parameters and gender-related differences in this primate. The thighs proved to be the most suitable measurement localization, since systolic values were less variable (left 4.03 ± 2.90%, right 5.96 ± 2.77%) compared with the tail (12.7 ± 6.96%). BP values were similar in the morning and in the afternoon (P > 0.05). Data were highly reproducible within and between several sessions on three consecutive days (P > 0.05) as well as over the course of 20 months (P > 0.05). Furthermore, the measurement time for females was significantly shorter than for males (5:14 ± 1:59 min versus 6:50 ± 1:58 min, P = 0.007). Measurement recommendations for the common marmoset were successfully established. Standardized values enabled a reliable comparison of BP parameters, e.g. for cardiovascular, toxicological or metabolic research.

  8. Enzymatic etching of gold nanorods by horseradish peroxidase and application to blood glucose detection

    Science.gov (United States)

    Saa, Laura; Coronado-Puchau, Marc; Pavlov, Valeri; Liz-Marzán, Luis M.

    2014-06-01

    Gold nanorods (AuNRs) have become some of the most used nanostructures for biosensing and imaging applications due to their plasmon-related optical response, which is highly sensitive toward minute changes in the AuNR aspect ratio. In this context, H2O2 has been used to trigger the chemical etching of AuNRs, thereby inducing a decrease of their aspect ratio. However, special conditions and relatively high concentrations of H2O2 are usually required, preventing the applicability of the system for biodetection purposes. To overcome this limitation we have introduced a biocatalytic species, the enzyme horseradish peroxidase (HRP) that is able to induce a gradual oxidation of AuNRs in the presence of trace concentrations of H2O2. Interestingly, the presence of halide ions has also been found to be essential for this process. As a consequence, other enzymatic reactions, such as those catalyzed by glucose oxidase, can be easily coupled to HRP activity, allowing the detection of different amounts of glucose. On the basis of these findings, we developed a highly sensitive and simple colorimetric assay that can be read out by the naked eye and allows the detection of physiological glucose concentrations in human serum.Gold nanorods (AuNRs) have become some of the most used nanostructures for biosensing and imaging applications due to their plasmon-related optical response, which is highly sensitive toward minute changes in the AuNR aspect ratio. In this context, H2O2 has been used to trigger the chemical etching of AuNRs, thereby inducing a decrease of their aspect ratio. However, special conditions and relatively high concentrations of H2O2 are usually required, preventing the applicability of the system for biodetection purposes. To overcome this limitation we have introduced a biocatalytic species, the enzyme horseradish peroxidase (HRP) that is able to induce a gradual oxidation of AuNRs in the presence of trace concentrations of H2O2. Interestingly, the presence of

  9. 13 CFR 302.18 - Post-approval requirements.

    Science.gov (United States)

    2010-01-01

    ... GENERAL TERMS AND CONDITIONS FOR INVESTMENT ASSISTANCE § 302.18 Post-approval requirements. (a) General. A... the terms and conditions of the Investment Assistance, including any special terms and applicable Federal cost principles (collectively, “Post-Approval Requirements”). A Recipient's failure to comply...

  10. 40 CFR 1065.12 - Approval of alternate procedures.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Approval of alternate procedures. 1065.12 Section 1065.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS ENGINE-TESTING PROCEDURES Applicability and General Provisions § 1065.12 Approval...

  11. 7 CFR 52.53 - Approved identification.

    Science.gov (United States)

    2010-01-01

    ...) When graded against a U.S. grade standard, meet the quality requirements for U.S Grade C or better; (5) Meet applicable fill weight and/or drained weight, Brix or other characteristics of a commodity related... approved by USDA inspection service prior to use. (b) Inspection (Continuous) grade and inspection...

  12. 42 CFR 84.35 - Changes or modifications of approved respirators; issuance of modification of certificate of...

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Changes or modifications of approved respirators... modifications of approved respirators; issuance of modification of certificate of approval. (a) Each applicant may, if he desires to change any feature of an approved respirator, request a modification of...

  13. Pembrolizumab: first global approval.

    Science.gov (United States)

    Poole, Raewyn M

    2014-10-01

    Pembrolizumab [Keytruda(®) (US)], a humanized monoclonal antibody against the programmed death receptor-1 (PD-1) protein, has been developed by Merck & Co for the treatment of cancer. Pembrolizumab has received its first global approval for the treatment of advanced, unresectable or metastatic malignant melanoma in the US, for use in patients with disease progression after prior treatment with ipilimumab and, for BRAF V600 mutation-positive patients, a BRAF inhibitor. It is the first anti-PD-1 therapy to receive regulatory approval in the US, and is currently under regulatory review in the EU. This article summarizes the milestones in the development of pembrolizumab leading to this first approval for the treatment of malignant melanoma.

  14. Pomalidomide: first global approval.

    Science.gov (United States)

    Elkinson, Shelley; McCormack, Paul L

    2013-05-01

    Pomalidomide (Pomalyst(®)) is a small molecule analogue of thalidomide under development with Celgene Corporation for the oral treatment of haematological and connective tissue diseases. Pomalidomide has been approved in the USA and is awaiting approval in the EU for use with low-dose dexamethasone for the treatment of relapsed and refractory multiple myeloma that has progressed following at least two prior therapies, including lenalidomide and bortezomib. The efficacy and safety of pomalidomide as monotherapy in patients with relapsed and refractory multiple myeloma has also been evaluated in a phase III trial. The agent is in phase III clinical development for the treatment of myelofibrosis and in phase II development for systemic sclerosis. Pomalidomide is also being investigated in patients with amyloidosis, prostate cancer, small cell lung cancer, pancreatic cancer, graft-versus-host disease, and Waldenstrom's macroglobulinaemia. This article summarizes the milestones in the development of pomalidomide leading to this first global approval for relapsed and refractory multiple myeloma.

  15. Apremilast: first global approval.

    Science.gov (United States)

    Poole, Raewyn M; Ballantyne, Anita D

    2014-05-01

    Apremilast (Otezla(®)), an oral small molecule inhibitor of type-4 cyclic nucleotide phosphodiesterase (PDE-4), is under development with Celgene Corporation for the treatment of psoriatic arthritis, psoriasis, ankylosing spondylitis, Behçet's syndrome, atopic dermatitis, and rheumatoid arthritis. Apremilast is indicated for the treatment of active psoriatic arthritis in adults. Apremilast has received its first global approval for this indication in the USA. Regulatory submissions for approval in this indication are under review in Canada and Europe. Regulatory filings have also been submitted for apremilast in the treatment of plaque psoriasis in the USA and Europe. This article summarizes the milestones in the development of apremilast leading to its first approval for the treatment of psoriatic arthritis.

  16. Application of Blood Appointment System in Hospital%血液预约系统在医院的应用

    Institute of Scientific and Technical Information of China (English)

    王飞; 李刚荣; 蒋天伦

    2011-01-01

    目的:通过血液预约,缓解血液供需关系.方法:应用现代信息技术手段,通过血液信息管理系统与医院信息管理系统的无缝连接及互联网优势,制订血液采集计划,招募献血者,控制血液库存.结果:规范了临床用血,缩短了血液在血库的流动周期,降低了血液管理成本.结论:血液预约是缓解血液供需关系的有效途径.%Objective: according to blood appointment, to relieve the supply and demand of blood.Method: apply the modern information technology, through seamless connection of the blood information system and hospital information system, the advantage of internet, to develop the program of blood connection, and recruit blood donors to control blood inventory.Result: standard the clinic blood, shorten the flow cycle of blood in blood bank, reduce the blood management costs.Conclusion: blood appointment is effective way to relieve the supply and demand of blood.

  17. FDA Approval for Imiquimod

    Science.gov (United States)

    On July 15, 2004, the U.S. Food and Drug Administration (FDA) announced the approval of a new indication for Aldara® (imiquimod) topical cream for the treatment of superficial basal cell carcinoma (sBCC), a type of skin cancer.

  18. Heartland Winthrop Approval

    Science.gov (United States)

    This May 19, 2016 letter from EPA approves the petition from Heartland Corn Products regarding non-grandfathered ethanol produced through a dry mill process, qualifying under the Clean Air Act for renewable fuel (D-code 6) RINs under the RFS program.

  19. FHR Iowa Falls Approval

    Science.gov (United States)

    This October 22, 2015, letter from EPA approves the petition from Flint Hills Resources, LLC, regarding non-grandfathered ethanol produced through the FHR Iowa Falls Process, qualifying under the Clean Air Act for renewable fuel (D-code 6) RINs under the R

  20. Blood smear

    Science.gov (United States)

    ... some red blood cells shaped like spheres ( hereditary spherocytosis ) Increased breakdown of RBCs Presence of RBCs with ... normal Red blood cells, elliptocytosis Red blood cells, spherocytosis Acute lymphocytic leukemia - photomicrograph Red blood cells, multiple ...

  1. Blood culture

    Science.gov (United States)

    Culture - blood ... A blood sample is needed . The site where blood will be drawn is first cleaned with an antiseptic such ... organism from the skin getting into (contaminating) the blood sample and causing a false-positive result (see ...

  2. Blood Thinners

    Science.gov (United States)

    If you have some kinds of heart or blood vessel disease, or if you have poor blood flow to your brain, your doctor may recommend that you take a blood thinner. Blood thinners reduce the risk of heart ...

  3. Blood transfusions

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000431.htm Blood transfusions To use the sharing features on this ... several sources of blood which are described below. Blood From the Public (Volunteer Blood Donation) The most ...

  4. Double-echo perfusion-weighted MR imaging: basic concepts and application in brain tumors for the assessment of tumor blood volume and vascular permeability

    Energy Technology Data Exchange (ETDEWEB)

    Uematsu, Hidemasa [University of Fukui, Department of Radiology, Faculty of Medical Sciences, Fukui (Japan); Maeda, Masayuki [Mie University School of Medicine, Department of Radiology, Mie (Japan)

    2006-01-01

    Perfusion-weighted magnetic resonance (MR) imaging using contrast agents plays a key role in characterizing tumors of the brain. We have shown that double-echo perfusion-weighted MR imaging (DEPWI) is potentially useful in assessing brain tumors. Quantitative indices, such as tumor blood volume, are obtained using DEPWI, which allows correction of underestimation of tumor blood volume due to leakage of contrast agents from tumor vessels, in addition to simultaneous acquisition of tumor vessel permeability. This article describes basic concepts of DEPWI and demonstrates clinical applications in brain tumors. (orig.)

  5. Localized T2 measurements using an OSIRIS-CPMG method. Application to measurements of blood oxygenation and transverse relaxation free of diffusion effect.

    Science.gov (United States)

    Girard, F; Poulet, P; Namer, I J; Steibel, J; Chambron, J

    1994-12-01

    This work presents a new method allowing localized T2 measurements, based upon the OSIRIS scheme. A train of 180 degrees pulses is applied after the OSIRIS preparation cycle, recording directly the transverse magnetization decay. The method was verified for two nuclei, 1H and 19F, with phantoms and in vivo on rats. The accuracy of the T2 values is discussed, as well as possible applications of the OSIRIS-CPMG method to proton transverse spin relaxation measurements, free of diffusion effects, and to non-invasive in vivo blood oxygenation measurements, through the use of an emulsion of perfluorooctylbromide, a blood substitute containing fluorine.

  6. Linaclotide: first global approval.

    Science.gov (United States)

    McWilliams, Vanessa; Whiteside, Glenn; McKeage, Kate

    2012-11-12

    Linaclotide is a once-daily, orally administered, first-in-class agonist of guanylate cyclase-C that is minimally absorbed. It is being developed to treat gastrointestinal disorders by Ironwood Pharmaceuticals and its partners, Forest Laboratories (North America), Almirall (Europe) and Astellas Pharma (Asia-Pacific). Linaclotide has received its first global approval in the US for the treatment of constipation-predominant irritable bowel syndrome (IBS-C) and chronic idiopathic constipation (CIC), and a marketing submission has been filed in the EU for IBS-C. This article summarizes the milestones in the development of linaclotide leading to this first approval for IBS-C and CIC. This profile has been extracted and modified from the Adis R&D Insight drug pipeline database. Adis R&D Insight tracks drug development worldwide through the entire development process, from discovery, through pre-clinical and clinical studies to market launch.

  7. Pitolisant: First Global Approval.

    Science.gov (United States)

    Syed, Yahiya Y

    2016-09-01

    Pitolisant (Wakix™) is an inverse agonist of the histamine H3 receptor that is being developed by Bioproject. Oral pitolisant is approved in the EU for the treatment of narcolepsy with or without cataplexy in adults. Pitolisant has received a Temporary Authorization of Use in France for this indication in case of treatment failure, intolerance or contraindication to currently available treatment. Pitolisant has orphan drug designation in the EU and the USA. In the pivotal HARMONY I trial, pitolisant significantly decreased excessive daytime sleepiness versus placebo in adults with narcolepsy with or without cataplexy (primary endpoint). Pitolisant also significantly decreased cataplexy rate versus placebo in these patients. This article summarizes the milestones in the development of pitolisant leading to this first approval for narcolepsy.

  8. 全自动血型分析仪在血站血型筛查工作中的应用%Application of fully automatic blood analyzer in blood type screening work in blood stations

    Institute of Scientific and Technical Information of China (English)

    武丽娟

    2015-01-01

    目的:探讨并评价全自动血型分析仪应用于血站献血者血型筛查的可行性。方法采用全自动血型分析仪(全自动法)对20335例献血者标本进行ABO及Rh(D)血型鉴定、盐水不规则抗体初筛,并与U 型微板法(半自动法)进行比对试验。结果全自动法与半自动法比较,ABO血型1次准确定型率:99.93%>98.84%,差异无统计学意义( P>0.05);Rh‐D血型鉴定正确率均为100.0%;O细胞凝集阳性率:0.13%>0.06%,差异有统计学意义( P<0.05);可保持反定型检测准确度达100.0%的血浆稀释倍数为1∶64,高于1∶4;脂血标本和溶血标本导致的误判率差异有统计学意义(P<0.05)。结论全自动血型分析仪具有较高的准确度、灵敏度及抗干扰能力,更易发现盐水不规则抗体,适合于血站开展献血者血型筛查工作。%Objective To research and evaluate the feasibility for applying the fully automatic blood analyzer in donators′blood type screening in blood station .Methods The fully automatic blood analyzer (automatic method) was adopted to conduct the ABO and Rh‐D blood typing and brine irregular antibody preliminary screening in 20 335 specimens of blood donors ,and then the detection results were compared with those detected by using the U micro‐plate method (semi‐automatic contrast test) .Results Comparing the fully automatic method with the semi‐automatic method ,the once accurate typing rate of ABO type:99 .93% >98 .84% ,the difference had no statistical significance (P>0 .05);the accuracy rate of Rh(D)type identification was 100 .0% ;the positive rate of O cell agglutination:0 . 13% >0 .06% ,the difference was statistically significant(P<0 .05);the plasma dilution ratio of keeping the reverse typing detection′s accuracy up to 100 .0% was 1∶64 ,higher than 1∶4;the difference in the misjudgment rate caused by hemolysis and lipemia

  9. Baricitinib: First Global Approval.

    Science.gov (United States)

    Markham, Anthony

    2017-03-13

    Baricitinib (Olumiant™) is an orally-administered, small-molecule, janus-associated kinase (JAK) inhibitor developed by Eli Lilly and Incyte Corporation for the treatment of rheumatoid arthritis (RA), atopic dermatitis and systemic lupus erythematosus. JAKs transduce intracellular signals from cell surface receptors for various cytokines and growth factors involved in inflammation and immune function, suggesting JAK inhibitors may be of therapeutic benefit in inflammatory conditions. In February 2017, baricitinib was approved in the EU, as monotherapy or in combination with methotrexate, for the treatment of moderate to severe active rheumatoid arthritis in adult patients who have responded inadequately to, or who are intolerant to one or more disease-modifying anti-rheumatic drugs (DMARDs). Regulatory approval to market baricitinib as a treatment for RA has also been sought in the USA and Japan. This article summarizes the milestones in the development of baricitinib leading to this first global approval for the treatment for moderate to severe active RA in adult patients who have responded inadequately to, or are intolerant to one or more DMARDs.

  10. The effect of nasal application of cocaine/adrenaline on blood loss in Le Fort I osteotomies

    NARCIS (Netherlands)

    de Lange, J.; Baas, E.M.; Horsthuis, R.B.G.; Booij, A.

    2008-01-01

    Cocaine is a very potent vasoconstrictor that is used by ENT specialists to reduce blood loss and enhance visibility during nasal surgery. In orthognathic surgery, especially Le Fort I procedures, excessive blood loss is a relatively frequent complication. In this study, a prospective randomized cli

  11. Carbon isotopes profiles of human whole blood, plasma, red blood cells, urine and feces for biological/biomedical 14C-accelerator mass spectrometry applications.

    Science.gov (United States)

    Kim, Seung-Hyun; Chuang, Jennifer C; Kelly, Peter B; Clifford, Andrew J

    2011-05-01

    Radiocarbon ((14)C) is an ideal tracer for in vivo human ADME (absorption, distribution, metabolism, elimination) and PBPK (physiological-based pharmacokinetic) studies. Living plants peferentially incorporate atmospheric (14)CO(2) versus (13)CO(2) versus (12)CO(2), which result in unique signature. Furthermore, plants and the food chains they support also have unique carbon isotope signatures. Humans, at the top of the food chain, consequently acquire isotopic concentrations in the tissues and body fluids depending on their dietary habits. In preparation of ADME and PBPK studies, 12 healthy subjects were recruited. The human baseline (specific to each individual and their diet) total carbon (TC) and carbon isotope (13)C (δ(13)C) and (14)C (F(m)) were quantified in whole blood (WB), plasma, washed red blood cell (RBC), urine, and feces. TC (mg of C/100 μL) in WB, plasma, RBC, urine, and feces were 11.0, 4.37, 7.57, 0.53, and 1.90, respectively. TC in WB, RBC, and feces was higher in men over women, P < 0.05. Mean δ(13)C were ranked low to high as follows: feces < WB = plasma = RBC = urine, P < 0.0001. δ(13)C was not affected by gender. Our analytic method shifted δ(13)C by only ±1.0 ‰ ensuring our F(m) measurements were accurate and precise. Mean F(m) were ranked low to high as follows: plasma = urine < WB = RBC = feces, P < 0.05. F(m) in feces was higher for men over women, P < 0.05. Only in WB, (14)C levels (F(m)) and TC were correlated with one another (r = 0.746, P < 0.01). Considering the lag time to incorporate atmospheric (14)C into plant foods (vegetarian) and or then into animal foods (nonvegetarian), the measured F(m) of WB in our population (recruited April 2009) was 1.0468 ± 0.0022 (mean ± SD), and the F(m) of WB matched the (extrapolated) atmospheric F(m) of 1.0477 in 2008. This study is important in presenting a procedure to determine a baseline for a study group for human ADME and PBPK studies using (14)C as a tracer.

  12. A signal processing application for evaluating self-monitoring blood glucose strategies in a software agent model.

    Science.gov (United States)

    Wang, Zhanle; Paranjape, Raman

    2015-07-01

    We propose the signal processing technique of calculating a cross-correlation function and an average deviation between the continuous blood glucose and the interpolation of limited blood glucose samples to evaluate blood glucose monitoring frequency in a self-aware patient software agent model. The diabetic patient software agent model [1] is a 24-h circadian, self-aware, stochastic model of a diabetic patient's blood glucose levels in a software agent environment. The purpose of this work is to apply a signal processing technique to assist patients and physicians in understanding the extent of a patient's illness using a limited number of blood glucose samples. A second purpose of this work is to determine an appropriate blood glucose monitoring frequency in order to have a minimum number of samples taken that still provide a good understanding of the patient's blood glucose levels. For society in general, the monitoring cost of diabetes is an extremely important issue, and these costs can vary tremendously depending on monitoring approaches and monitoring frequencies. Due to the cost and discomfort associated with blood glucose monitoring, today, patients expect monitoring frequencies specific to their health profile. The proposed method quantitatively assesses various monitoring protocols (from 6 times per day to 1 time per week) in nine predefined categories of patient agents in terms of risk factors of health status and age. Simulation results show that sampling 6 times per day is excessive, and not necessary for understanding the dynamics of the continuous signal in the experiments. In addition, patient agents in certain conditions only need to sample their blood glucose 1 time per week to have a good understanding of the characteristics of their blood glucose. Finally, an evaluation scenario is developed to visualize this concept, in which appropriate monitoring frequencies are shown based on the particular conditions of patient agents. This base line can

  13. 武汉协和医院血库信息系统的设计与应用%Designing and Application of Blood Bank Information System

    Institute of Scientific and Technical Information of China (English)

    程超; 周彬

    2011-01-01

    With the development of information technology, blood bank information system has been continuously improved and it was applied generally. It describes that the design and application of blood bank information system. The integration of blood bank system with HIS were completed, the function of blood application from medical workstation system was implemented. It optimizes the patient's medical treatment processes and improves the efficiency and the quality of medical services.%随着信息技术的发展,血库信息系统不断完善并在医院得到广泛应用.本文就血库信息系统的设计方法和应用效果做了详细阐述.完成血库信息系统与医院信息系统融合,实现了通过住院医生工作站提交输血申请的功能,同时依托网络将申请信息传送给血库人员.血库信息系统的使用优化了工作流程,提高了服务质量,保证了用血安全.

  14. Using 3G VPN Technology to Expand Applications in Blood Banks%3G VPN技术在采供血机构的拓展应用

    Institute of Scientific and Technical Information of China (English)

    季节; 张剑

    2013-01-01

    Through elaborating 3G VPN technology applications in blood banks to expand the application of significance, technical principles and significant effect on blood collection agencies aimed at improving understanding of technology 3G VPN taken to further promote 3G VPN blood banks in the full implementation and continue to expand and perfect for blood transfusion services in the field tend to be more efficient and safe.%通过阐述3G VPN技术在采供血机构拓展应用的适用意义、技术原理及明显效果,目的在于提高对采供血机构采取3G VPN技术的认识,进一步促进3G VPN在采供血机构的全面推行,并不断拓展与完善,以便采供血领域的服务更加趋于快捷安全。

  15. Blood Donation

    Science.gov (United States)

    Tests and Procedures Blood donation By Mayo Clinic Staff Blood donation is a voluntary procedure. You agree to have blood drawn so that it can ... have a disease that requires blood components. Blood donation makes all of this possible. There are several ...

  16. Pharmaceutical Approval Update

    OpenAIRE

    Gohil, Kunj

    2015-01-01

    Evolocumab (Repatha) for patients with hypercholesterolemia whose condition has not been controlled by statins and other therapies; trifluridine/tipiracil (Lonsurf) for metastatic colorectal cancer; and blood coagulation factor VIII (Nuwiq) for adults and children with hemophilia A

  17. [Joint application of mathematic models in assessing the residual risk of hepatitis C virus transmitted through blood transfusion].

    Science.gov (United States)

    Wang, Xun; Jia, Yao; Xie, Yun-zheng; Li, Xiu-mei; Liu, Xiao-ying; Wu, Xiao-fei

    2011-09-01

    The practicable and effective methods for residual risk assessment on transfusion-transmitted disease was to establish the mathematic models. Based on the characteristics of the repeat donors which donated their blood on a regular base, a model of sero-conversion during the interval of donations was established to assess the incidence of the repeat donors. Based on the characteristics of the prevalence in the population, a model of 'prevalence increased with the age of the donor' was established to assess the incidence of those first-time donors. And based on the impact of the windows period through blood screening program, a model of residual risk associated with the incidence and the length of the windows period was established to assess the residual risk of blood transfusion. In this paper, above said 3 kinds of mathematic models were jointly applied to assess the residual risk of hepatitis C virus (HCV) which was transmitted through blood transfusion in Shanghai, based on data from the routine blood collection and screening program. All the anti-HCV unqualified blood donations were confirmed before assessment. Results showed that the residual risk of HCV transmitted through blood transfusion during Jan. 1(st), 2007 to Dec. 31(st), 2008 in Shanghai was 1:101 000. Data showed that the results of residual risk assessment with mathematic models was valuable. The residual risk of transfusion-transmitted HCV in Shanghai was at a safe level, according to the results in this paper.

  18. 78 FR 66826 - Prior Label Approval System: Generic Label Approval

    Science.gov (United States)

    2013-11-07

    ... submitted for evaluation and approval before use under the final rule are: (1) Labels for chicken produced..., such as ``no antibiotics administered'' or ``vegetarian fed''; (4) instructional or...

  19. Donating Blood

    Science.gov (United States)

    ... can't get an infection or disease from giving blood. The needles and other equipment used are sterile ... part of blood (plasma) within 72 hours after giving blood. It generally takes about 4–8 weeks to ...

  20. Study the Clinical Application of Irradiated Red Blood Cell Components%辐照血液成分的临床应用研究

    Institute of Scientific and Technical Information of China (English)

    徐伟; 孙美贞

    2015-01-01

    目的:探讨分析辐照血液成分的临床用量及所占比例。方法:统计分析青岛地区2010-2014年辐照血液成分的临床用血量及占总用血量的比例,对比分析近几年辐照血液成分比例变化。结果:青岛地区近5年临床用血量中辐照红细胞类成分占红细胞类总用血量的0.91%,辐照血小板类占血小板总用量的7.40%。与前两年相比,近3年辐照血液成分占总用血量的比例大幅增加。结论:随着对TA-GVHD认识的进一步深入,临床对辐照血液成分的重视程度加大,辐照血液成分的使用量也不断增加。%Objective:To analyze the clinical application and the proportion of irradiated red blood cell components.Method: The clinical application in irradiation blood components of Qingdao from 2010 to 2014 were statistical analyzed,the irradiated blood composition changes in recent years were compared and analyzed.Result:Nearly the five years, the percentage of irradiated red blood cells in the total clinical usage was 0.91%,and the percentage of irradiated aphaeresis platelet in the total clinical usage was 7.40%. Compared with the previous two years, a big increasion in the clinical usage of irradiated red blood cell components in the nearly three years.Conclusion:Along with the further understanding of TA-GVHD,clinical doctors pay more attention to the application in irradiation blood components and the use of irradiation blood components also increased.

  1. Forensic-metrological considerations on assessment of compliance (or non-compliance) in forensic blood alcohol content determinations: A case study with software application.

    Science.gov (United States)

    Zamengo, Luca; Frison, Giampietro; Tedeschi, Gianpaola; Frasson, Samuela

    2016-08-01

    Blood alcohol concentration is the most frequent analytical determination carried out in forensic toxicology laboratories worldwide. It is usually required to assess if an offence has been committed by comparing blood alcohol levels with specified legal limits, which can vary widely among countries. Due to possible serious legal consequences associated with non-compliant alcohol levels, measurement uncertainty should be carefully evaluated, along with other metrological aspects which can influence the final result. The whole procedure can be time-consuming and error-generating in routine practice, increasing the risks for unreliable assessments. A software application named Ethanol WorkBook (EtWB) was developed at the author's laboratory by using Visual Basic for Application language and MS Excel(®), with the aim of providing help to forensic analysts involved in blood alcohol determinations. The program can (i) calculate measurement uncertainties and decision limits with different methodologies; (ii) assess compliance to specification limits with a guard-band approach; (iii) manage quality control (QC) data and create control charts for QC samples; (iv) create control maps from real cases data archives; (v) provide laboratory reports with graphical outputs for elaborated data and (vi) create comprehensive searchable case archives. A typical example of drink driving case is presented and discussed to illustrate the importance of a metrological approach for reliable compliance assessment and to demonstrate software application in routine practice. The tool is made freely available to the scientific community at request.

  2. Application of the holographic interference microscope for investigation of ozone therapy influence on blood erythrocytes of patients in vivo

    Science.gov (United States)

    Tishko, Tatyana V.; Titar, V. P.; Barchotkina, T. M.; Tishko, D. N.

    2004-09-01

    The holographic methods of phase micro-objects visualization (the holographic phase contrast method and the method of holographic interferometry) are considered. Comparative analysis of classical and holographic methods in microscopy of phase micro-objects is carried out. An arrangement of the holographic interference microscope realizing the holographic methods and experimental results of 3-D imaging of native blood erythrocytes are presented. It is shown that 3-D morphology of blood erythrocytes reflects and determines the state of a human organism and those different physical and chemical factors and internal pathologies influence erythrocytes morphology. The holographic interference microscope was used for investigation of ozone therapy influence on human blood erythrocytes. Blood samples of 60 patients of different age with neurosensoric hardness of hearing before and after ozone therapy were investigated. It was shown that all patients have changed erythrocytes mrophology. Ozone therapy treatment results in normalization of erythrocytes morphology of patients.

  3. Obinutuzumab: first global approval.

    Science.gov (United States)

    Cameron, Fiona; McCormack, Paul L

    2014-01-01

    Obinutuzumab (Gazyva™) is an intravenously administered, humanized and glycoengineered, type II anti-CD20 monoclonal antibody for the treatment of B-cell malignancies. It is approved in the US for use in combination with chlorambucil for the first-line treatment of chronic lymphocytic leukaemia (CLL), and has been filed for approval in the EU in this indication. The antibody is based on GlycArt Biotechnology's (later Roche Glycart AG) proprietary GlycoMAb® technology, which uses glycoengineered antibodies that specifically increase antibody-dependent cellular cytotoxicity and thereby increase immune-mediated target cell death. Obinutuzumab is a type II anti-CD20 antibody that induces enhanced direct cell death. The monoclonal antibody is in worldwide phase III development with Roche and its subsidiaries, Genentech and Chugai Pharmaceutical, as well as Biogen Idec, for diffuse large B-cell lymphoma and non-Hodgkin's lymphoma generally, and is also in phase III development in countries outside of the US and EU for CLL.

  4. Safinamide: first global approval.

    Science.gov (United States)

    Deeks, Emma D

    2015-04-01

    Safinamide (Xadago(®)) is an oral α-aminoamide derivative developed by Newron for the treatment of Parkinson's disease (PD). The drug has both dopaminergic properties (highly selective and reversible inhibition of monoamine oxidase-B) and non-dopaminergic properties (selective sodium channel blockade and calcium channel modulation, with consequent inhibition of excessive glutamate release). Safinamide is approved in the EU, Iceland, Lichtenstein and Norway, as an add-on therapy to stable-dose levodopa, alone or in combination with other PD therapies in mid- to late-stage fluctuating PD patients; regulatory submissions have also been filed in the USA and Switzerland for its use in this indication. Additional submissions have been made in the USA, Iceland, Lichtenstein, Norway and Switzerland for early-stage PD. Safinamide has also undergone phase II investigation in PD patients with drug-induced dyskinesia (France, Germany, Austria, Canada and South Africa) or cognitive impairment (USA and Spain). This article summarizes the milestones in the development of safinamide leading to its first approval for PD.

  5. Drugs Approved for Cervical Cancer

    Science.gov (United States)

    ... Human Papillomavirus (HPV) Nonavalent Vaccine Recombinant Human Papillomavirus (HPV) Quadrivalent Vaccine Drugs Approved to Treat Cervical Cancer Avastin (Bevacizumab) Bevacizumab Blenoxane (Bleomycin) Bleomycin Hycamtin (Topotecan ...

  6. Roche Portable Blood Glucose Meter for Clinical Application and Quality Control%便携式血糖仪临床应用与质量控制

    Institute of Scientific and Technical Information of China (English)

    谭丹华

    2014-01-01

    Diabetes is a lifelong disease is stil no cure at present, timing determination of glucose, strict control of blood glucose is the key to the treatment of diabetes melitus. Diabetes ideal blood glucose test is weekly 1~2 times, but generaly patients with no conditions to the hospital for frequent blood glucose testing, and the portable blood glucose meter provides great convenience for patients, the portable blood glucose meter for each ward monitoring blood glucose in patients with circadian changes and outpatient diabetes screening, increasingly the attention of medical workers and patients in this paper, Glucose meter in the clinical diagnosis of practical value in clinical application, quality assessment.%糖尿病是目前尚不能根治的终身性疾病,定时测定血糖值,严格控制血糖是糖尿病治疗的关键。糖尿病理想的血糖测试是每周1~2次,但一般患者没条件到医院作频繁的血糖测试,而便携式血糖仪为患者提供了很大的方便。便携式血糖仪因适合各病房昼夜监测患者血糖变化及门诊患者糖尿病筛查,日益受到医务工作者和患者的重视,本文从临床应用、质量检测评估方面阐述血糖仪在临床诊断的实用价值。

  7. Application of mobile blood purification system in the treatment of acute renal failure dog model in the field environment

    Directory of Open Access Journals (Sweden)

    Zhi-min ZHANG

    2014-01-01

    Full Text Available Objective To evaluate the stability, safety and efficacy of mobile blood purification system in the treatment of acute renal failure dog model in the field environment. Methods The acute renal failure model was established in 4 dogs by bilateral nephrectomy, which was thereafter treated with the mobile blood purification system. The evaluation of functional index of the mobile blood purification system was performed after a short-time (2 hours and conventional (4 hours dialysis treatment. Results The mobile blood purification system ran stably in the field environment at a blood flow of 150-180ml/min, a dialysate velocity of 2000ml/h, a replacement fluid velocity of 2000ml/h, and ultrafiltration rate of 100-200ml/h. All the functions of alarming system worked well, including static upper limit alarm of ultrafiltration pressure (>100 mmHg, upper limit alarm of ambulatory arterial pressure (>400mmHg, upper limit alarm of ambulatory venous pressure (>400mmHg, bubble alarm of vascular access, bubble alarm during the infusion of solutions, pressure alarm at the substitution pump segment and blood leaking alarm. The vital signs of the 4 dogs with acute renal failure kept stable during the treatment. After the treatment, a remarkable decrease was seen in the levels of serum urea nitrogen, creatinine and serum potassium (P0.05. Conclusions The mobile blood purification system runs normally even in a field environment. It is a flexible and portable device with a great performance in safety and stability in the treatment of acute renal failure. DOI: 10.11855/j.issn.0577-7402.2013.12.15

  8. 发酵血粉营养特性及其应用%Nutritional Characteristics and Application of Fermented Blood Meal

    Institute of Scientific and Technical Information of China (English)

    苏双良; 谷子林; 刘亚娟

    2011-01-01

    With the rapid development of breeding industry, feed resources, especially poor protein resource has become one bottleneck to develop animal husbandry. In the development and utilization of unconventional animal protein feed, blood meal is one promising feed. Fermented blood meal is recognized to be the direction for blood meal development in China. Nutritional characteristics of fermented blood meal and its application in animal production were discussed in this paper.%随着养殖业的迅猛发展,蛋白饲料资源普遍贫乏已经成为发展畜牧业瓶颈之一。在非常规动物蛋白质饲料的开发利用中,血粉是大有前途的动物性蛋白饲料,而发酵血粉被公认为我国饲用血粉开发的一个重要方向。文章就发酵血粉营养特性及其在动物生产中的应用作一综述。

  9. [THE POSSIBILITIES OF APPLICATION OF TECHNOLOGY PROTEIN MICROARRAY (MICROCHIPS) FOR ANALYSIS OF PROTEIN COMPOSITION OF BLOOD SERUM].

    Science.gov (United States)

    Gumanova, N G; Klimushina, M V; Metelskaya, V A; Boitsov, S A

    2015-10-01

    The microchip technology represents convenient and relatively economic tool of analyzing specific biomarkers with the purpose to diagnose diseases, to evaluate effectiveness of therapy and to investigate signaling pathways. To analyze protein composition of blood serum certain types of finished microchips which were not applied previously on the territory of Russia. The detection from 2% to 5% out of matrix of chips depending on their variety was managed without preliminary depletion of serum (removal of proteins of major fractions). Hence, partial protein composition of blood serum can be analyzed with microchips even without preliminary removal of proteins of major fractions.

  10. 78 FR 76383 - Notice of Passenger Facility Charge (PFC) Approvals and Disapprovals

    Science.gov (United States)

    2013-12-17

    ... Airport Authority, Mobile, Alabama. Application Number: 13-06-C-00-MOB. Application Type: Impose and use a... Regional Airport (MOB). Determination: Approved. Based on information submitted in the public agency's... annual enplanements at MOB. Brief Description of Projects Approved for Collection and Use:...

  11. Detection of Neisseria meningitidis from Negative Blood Cultures and Cerebrospinal Fluid with the FilmArray Blood Culture Identification Panel

    OpenAIRE

    Pardo, Joe; Klinker, Kenneth P.; Borgert, Samuel J.; Butler, Brittany M.; Rand, Kenneth H.; Iovine, Nicole M.

    2014-01-01

    The FilmArray blood culture identification (BCID) panel is a rapid molecular diagnostic test approved for use with positive blood culture material. We describe a fatal case of meningococcemia with central nervous system (CNS) involvement detected using the BCID test with culture-negative blood and cerebrospinal fluid.

  12. Detection of Neisseria meningitidis from negative blood cultures and cerebrospinal fluid with the FilmArray blood culture identification panel.

    Science.gov (United States)

    Pardo, Joe; Klinker, Kenneth P; Borgert, Samuel J; Butler, Brittany M; Rand, Kenneth H; Iovine, Nicole M

    2014-06-01

    The FilmArray blood culture identification (BCID) panel is a rapid molecular diagnostic test approved for use with positive blood culture material. We describe a fatal case of meningococcemia with central nervous system (CNS) involvement detected using the BCID test with culture-negative blood and cerebrospinal fluid.

  13. FDA-Approved HIV Medicines

    Science.gov (United States)

    HIV Treatment FDA-Approved HIV Medicines (Last updated 2/27/2017; last reviewed 2/27/2017) Treatment with HIV medicines is ... approved by the U.S. Food and Drug Administration (FDA) for the treatment of HIV infection in the ...

  14. The application of cassette automatic blood analyzer in irregular antibody screening for blood donors%卡式全自动血型仪在献血者不规则抗体筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    邹姣丽; 王庆; 邹文涛; 何子毅; 王若珩; 陈庆恺; 崔四平

    2015-01-01

    目的:比较卡式全自动血型仪法与传统血清学法对不规则抗体筛查的应用效果。方法分别采用卡式全自动血型仪法与传统血清学方法对2013年10月1日至2014年4月30日东莞市无偿献血者45709份标本进行不规则抗体筛查,对筛查阳性的标本采用抗球蛋白法进行鉴定,比较两种方法检出不规则抗体的性能,并对献血者不规则抗体分布进行分析。结果卡式全自动血型仪检出不规则抗体阳性95例,检出率为0.208%,传统血清学法检出不规则抗体16例,检出率0.035%,差异有统计学意义( P<0.05);卡式全自动血型仪法阳性检出符合率为56.547%,高于传统血清学法阳性检出符合率28.571%( P<0.05);RhD阴性献血者不规则抗体阳性率为2.242%,高于RhD阳性献血者不规则抗体率0.198%(P<0.05)。结论卡式全自动血型仪法鉴定献血者红细胞不规则抗体的阳性检出率高于传统血清学法,对RhD阴性献血者更应进行不规则抗体的筛查,献血者红细胞不规则抗体类型以Ig M型无临床意义的抗体为主。%Objective To compare the application effect between the method of cassette automatic blood analyzer and the method of traditional serological .Methods Using the method of cassette automatic blood analyzer and the method of traditional serological , respectively ,the samples of blood donors in Dongguan from October 1 ,2013 to April 30 ,2014 were performed irregular antibody screening .The positive samples of screening were identified by using antiglobulin method ,and the irregular antibodies of blood do‐nors were analyzed .Results There were 95 positive cases of irregular antibody by the method of cassette automatic blood analyzer , and the detection rate was 0 .208% .There were 16 positive cases of irregular antibody by the method of traditional serological ,the detection rate was 0 .035% ,and there was

  15. Application of solid-phase radioimmunoassay in determining antibodies to Aujeszky's disease virus in blood serum of vaccinated pigs

    Energy Technology Data Exchange (ETDEWEB)

    Rodak, L.; Smid, B.; Valicek, L. (Vyzkumny Ustav Veterinarniho Lekarstvi, Brno-Medlanky (Czechoslovakia))

    1983-11-01

    In the blood sera of pigs vaccinated with inactivated vaccines manufactured by three different manufacturers the RIA method was used to determine the specific antibodies to the virus of Aujeszky's disease. In certain groups of vaccinated pigs the results of the RIA examination are unfavourably affected by the bond of antibodies to the cellular antigenous determinants. This proves that following vaccination antibodies are formed not only against the viral antigen but also against the antigens of cells on which the vaccination virus is propagated. These shortcomings are eliminated by the use of suitable cellular cultures for the preparation of viral and control antigens. Antigens are applicable for RIA and for ELISA examinations of blood sera of infected and vaccinated pigs. The advantages are described of the RIA and ELISA methods as compared with the virus neutralization test.

  16. 医院科技人员请假审批系统的研制与应用%Development and Application of Leave Approval System for Technical Professionals of the Hospital

    Institute of Scientific and Technical Information of China (English)

    刘同波; 邱明辉; 郭旭

    2012-01-01

    The management of staff applying for a leave is difficult for the administration department of our hospital. This paper introduces a web-based staff leave approval system through the hospital internal network. The design and implementation technology of the system are presented in detail. The application status and effects of the system are summarized. The system has been operating for two years which helps improve work efficiency for both high-level managers and ordinary employees. The system is well received by all hospital users.%大型综合型医院科技人员外出请假管理一直是困扰管理部门的一个难题.介绍了解放军总医院自行研制的基于浏览器方式的人员外出请假审批系统,详细描述了系统的设计和实现技术,并对系统的应用情况和应用效果进行了总结.系统在医院稳定运行近两年,收到了良好效果,具有广泛应用价值.

  17. Analysis of positive result of blood culture with BACTEC FX automatic blood culture system and its application%BACTEC FX 全自动血培养仪阳性结果分析及应用评价

    Institute of Scientific and Technical Information of China (English)

    麦珍; 朱雄; 陈海; 李欢; 黎元莉; 黎礼达

    2014-01-01

    目的:对美国BD公司 BACTEC FX全自动血培养仪进行血培养结果分析,并对其临床应用进行评价。方法收集2011年10月-2012年11月医院收治怀疑血流感染患者的血液标本,血培养及鉴定采用美国BD公司BACTEC FX全自动血培养仪和BD Phoenix l00自动细菌鉴定仪。结果2590份血液标本中实际检出阳性标本264份,阳性率10.2%,假阳性率0.15%;264株病原菌中,最快检出时间为2.7 h ,有26.9%在12 h内检出,51.1%在24 h内检出,86.0%在48 h内检出,95.8%在72 h内检出;有40.5%标本厌氧瓶先报警、需氧瓶后报警,34.5%标本需氧瓶先报警、厌氧瓶后报警,11.8%标本需氧瓶先报警、但厌氧瓶不报警。结论美国BD公司BACTEC FX全自动血培养仪操作简便、灵敏度高,提高了细菌检出的阳性率及种类,检出速度更快捷、更准确,为治疗患者赢得了宝贵的时间。%OBJECTIVE To analyze the result of blood culture with BACTEC FX automatic blood culture system of BD company of America and evaluate the clinical application .METHODS The blood specimens were collected from the patients with suspected bloodstream infections who were treated in the hospital from Oct 2011 to Nov 2012 , then the blood culture and the identification of bacteria were performed with the use of BACTEC FX automatic blood culture system of BD company of America and the BD Phoenix l00 automatic bacteria identification system . RESULTS Of the 2 590 blood specimens ,264 were actually cultured positive with the positive rate of 10 .2% and the false positive rate of 0 .15% .Among the 264 strains of isolated pathogens ,the rapidest detection time was 2 .7 hours ,26 .9% of the pathogens were detected within 12 hours ,51 .1% detected within 24 hours ,86 .0% detected within 48 hours ,95 .8% detected within 72 hours .40 .5% of the specimens firstly alarmed in the anaerobic bottles then in the aerobic bottles

  18. Inflow/Outflow Boundary Conditions for Particle-Based Blood Flow Simulations: Application to Arterial Bifurcations and Trees.

    Science.gov (United States)

    Lykov, Kirill; Li, Xuejin; Lei, Huan; Pivkin, Igor V; Karniadakis, George Em

    2015-08-01

    When blood flows through a bifurcation, red blood cells (RBCs) travel into side branches at different hematocrit levels, and it is even possible that all RBCs enter into one branch only, leading to a complete separation of plasma and RBCs. To quantify this phenomenon via particle-based mesoscopic simulations, we developed a general framework for open boundary conditions in multiphase flows that is effective even for high hematocrit levels. The inflow at the inlet is duplicated from a fully developed flow generated in a pilot simulation with periodic boundary conditions. The outflow is controlled by adaptive forces to maintain the flow rate and velocity gradient at fixed values, while the particles leaving the arteriole at the outlet are removed from the system. Upon validation of this approach, we performed systematic 3D simulations to study plasma skimming in arterioles of diameters 20 to 32 microns. For a flow rate ratio 6:1 at the branches, we observed the "all-or-nothing" phenomenon with plasma only entering the low flow rate branch. We then simulated blood-plasma separation in arteriolar bifurcations with different bifurcation angles and same diameter of the daughter branches. Our simulations predict a significant increase in RBC flux through the main daughter branch as the bifurcation angle is increased. Finally, we demonstrated the effectiveness of the new methodology in simulations of blood flow in vessels with multiple inlets and outlets, constructed using an angiogenesis model.

  19. Insight into "Calculated Risk": An Application to the Prioritization of Emerging Infectious Diseases for Blood Transfusion Safety.

    Science.gov (United States)

    Neslo, R E J; Oei, W; Janssen, M P

    2017-02-23

    Increasing identification of transmissions of emerging infectious diseases (EIDs) by blood transfusion raised the question which of these EIDs poses the highest risk to blood safety. For a number of the EIDs that are perceived to be a threat to blood safety, evidence on actual disease or transmission characteristics is lacking, which might render measures against such EIDs disputable. On the other hand, the fact that we call them "emerging" implies almost by definition that we are uncertain about at least some of their characteristics. So what is the relative importance of various disease and transmission characteristics, and how are these influenced by the degree of uncertainty associated with their actual values? We identified the likelihood of transmission by blood transfusion, the presence of an asymptomatic phase of infection, prevalence of infection, and the disease impact as the main characteristics of the perceived risk of disease transmission by blood transfusion. A group of experts in the field of infectious diseases and blood transfusion ranked sets of (hypothetical) diseases with varying degrees of uncertainty associated with their disease characteristics, and used probabilistic inversion to obtain probability distributions for the weight of each of these risk characteristics. These distribution weights can be used to rank both existing and newly emerging infectious diseases with (partially) known characteristics. Analyses show that in case there is a lack of data concerning disease characteristics, it is the uncertainty concerning the asymptomatic phase and the disease impact that are the most important drivers of the perceived risk. On the other hand, if disease characteristics are well established, it is the prevalence of infection and the transmissibility of the disease by blood transfusion that will drive the perceived risk. The risk prioritization model derived provides an easy to obtain and rational expert assessment of the relative importance of

  20. Artificial blood

    Directory of Open Access Journals (Sweden)

    Sarkar Suman

    2008-01-01

    Full Text Available Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone.

  1. Artificial blood.

    Science.gov (United States)

    Sarkar, Suman

    2008-07-01

    Artificial blood is a product made to act as a substitute for red blood cells. While true blood serves many different functions, artificial blood is designed for the sole purpose of transporting oxygen and carbon dioxide throughout the body. Depending on the type of artificial blood, it can be produced in different ways using synthetic production, chemical isolation, or recombinant biochemical technology. Development of the first blood substitutes dates back to the early 1600s, and the search for the ideal blood substitute continues. Various manufacturers have products in clinical trials; however, no truly safe and effective artificial blood product is currently marketed. It is anticipated that when an artificial blood product is available, it will have annual sales of over $7.6 billion in the United States alone.

  2. [Applications in gynecology-obstetrics of the first legal cases concerning HIV transmission by blood transfusion and errors in screening].

    Science.gov (United States)

    Pierre, F; Soutoul, J H

    1991-01-01

    The intention of the authors in this paper is to provide a preliminary report on what is going on in order to inform gynaecologists and obstetricians of the initial legal steps concerning the transmission of HIV by blood transfusion and the defects in screening for the infection which could have been caught by a pregnant woman; where there was a possibility of a false-positive diagnosis from the serum which could have brought about a termination of pregnancy unnecessarily. Two decisions have been analysed, the one according to the old administrative law and the other according to the more recent judgement, involving pregnancy and other decisions which were not directly concerned with the specialty. These can be instructive for practitioners because these circumstance may come up again. It is worth looking at the case papers where medical responsibility can be invoked involving the professional insurance agencies. These cases will increase in numbers in the future. The authors analysed in the light of the decisions that had been made, possible penal, civil and administrative liabilities that could threaten gynaecologists and obstetricians in this field. The study ends by pointing out what can be done to lessen these medico-legal risks by avoiding blood loss in operations, by finding other ways than blood transfusions towards the end of pregnancy, but above all, by reducing and controlling the number of blood transfusions carefully. This has to be done in collaboration with the transfusion centres and the laboratories where the blood is tested. Judges will have to work out where the responsibility lies if the cases come before them.

  3. In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling.

    Science.gov (United States)

    Elliott, Winston; Scott-Drechsel, Devon; Tan, Wei

    2015-11-03

    Vascular disease is a common cause of death within the United States. Herein, we present a method to examine the contribution of flow dynamics towards vascular disease pathologies. Unhealthy arteries often present with wall stiffening, scarring, or partial stenosis which may all affect fluid flow rates, and the magnitude of pulsatile flow, or pulsatility index. Replication of various flow conditions is the result of tuning a flow pressure damping chamber downstream of a blood pump. Introduction of air within a closed flow system allows for a compressible medium to absorb pulsatile pressure from the pump, and therefore vary the pulsatility index. The method described herein is simply reproduced, with highly controllable input, and easily measurable results. Some limitations are recreation of the complex physiological pulse waveform, which is only approximated by the system. Endothelial cells, smooth muscle cells, and fibroblasts are affected by the blood flow through the artery. The dynamic component of blood flow is determined by the cardiac output and arterial wall compliance. Vascular cell mechano-transduction of flow dynamics may trigger cytokine release and cross-talk between cell types within the artery. Co-culture of vascular cells is a more accurate picture reflecting cell-cell interaction on the blood vessel wall and vascular response to mechanical signaling. Contribution of flow dynamics, including the cell response to the dynamic and mean (or steady) components of flow, is therefore an important metric in determining disease pathology and treatment efficacy. Through introducing an in vitro co-culture model and pressure damping downstream of blood pump which produces simulated cardiac output, various arterial disease pathologies may be investigated.

  4. 42 CFR 84.50 - Types of respirators to be approved; scope of approval.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Types of respirators to be approved; scope of... Classification of Approved Respirators; Scope of Approval; Atmospheric Hazards; Service Time § 84.50 Types of respirators to be approved; scope of approval. Approvals shall be issued for the types of respirators...

  5. Drugs Approved for Stomach (Gastric) Cancer

    Science.gov (United States)

    ... about Your Treatment Research Drugs Approved for Stomach (Gastric) Cancer This page lists cancer drugs approved by the ... are not listed here. Drugs Approved for Stomach (Gastric) Cancer Cyramza (Ramucirumab) Docetaxel Doxorubicin Hydrochloride 5-FU (Fluorouracil ...

  6. 75 FR 44047 - Notice of Passenger Facility Charge (PFC) Approvals and Disapprovals

    Science.gov (United States)

    2010-07-27

    ... of Sec. 158.29. PFC Applications Approved Public Agency: County and City of Spokane, Washington...--runway plow. Airfield friction tester. Snow removal equipment--airfield snow blower. PFC application...

  7. [The currently available possibilities for the application of photogrammetry in the forensic medical expertise of the blood stains at the scene of the crime].

    Science.gov (United States)

    Fetisov, V A; Makarov, I Yu; Gusarov, A A; Lorents, A S; Smirenin, S A; Stragis, V B

    2017-01-01

    The study of blood stains retained at the scene of the crime is of crucial importance for the preliminary inquiry. The present article is focused on the analysis of the possibilities and prospects for the use of photogrammetry (PM) as exemplified by the foreign expert practice of the blood stains examination at the site of the event. It is shown that the results of the application of digital photogrammetry in addition to the traditional methods of morphological investigations enables the forensic medical experts to reconstruct a number of unique features and circumstances that accompanied the commission of a crime at the site of the event. Such PM techniques supplemented by the ballistic analysis of the blood splatter and droplet trajectories provides additional evidence that allows the forensic medical experts to reconstruct the scene of the crime including the pose and position of the victim at the moment of causing injury. Moreover, these data make it possible to determine the maximum number and the sequence of injurious impacts (blows). The authors discuss the advantages and relative disadvantages of the application of the photogrammetric technique in the routine practical expert work. It is emphasized that the published decision making algorithms provide the specialists in various disciplines and professional experts with the ready-made technological tools for obtaining the additional criteria for the objective improvement of the quality of the studies they carry out and for the enhancement of the value of expert conclusions. It is concluded that the application of the modern photogrammetric technologies can be recommended for the solution of the applied forensic medical problems and conducting the relevant expert research.

  8. Integrative assessment of kick boxers’ brain blood circulation and bio-electrical activity in conditions of correction technologies’ application

    Directory of Open Access Journals (Sweden)

    Romanov Y.N.

    2016-06-01

    Full Text Available Purpose: to scientifically substantiate the role of para-vertebral impacts on blood circulation and bio-electrical activity of kick boxers’ cortex. Material: in the research participated kick boxers (main group, n=62 and university students (control group, n=25 of 18-23 years’ age. Assessment of para-vertebral impacts with device Armos and classic massage was fulfilled with the help of the following methodic: trans-cranial dopplerography of head main arteries and cortex EEG of the tested. Results: it was found that with the help of para-vertebral impacts by device Armos linear velocity of cerebral blood flow reduces to normal limits and in- and inter-hemispheres’ interaction strength increases. Conclusions: para-vertebral impacts by device Armos activate integrative processes and inter-hemispheres’ interactions of different cortex areas of kick boxers. It can witness about better formation of functional systems, ensuring sports efficiency.

  9. The Dopaminergic System in Peripheral Blood Lymphocytes: From Physiology to Pharmacology and Potential Applications to Neuropsychiatric Disorders

    OpenAIRE

    Buttarelli, Francesca R.; Fanciulli, Alessandra; Pellicano, Clelia; Pontieri, Francesco E.

    2011-01-01

    Besides its action on the nervous system, dopamine (DA) plays a role on neural-immune interactions. Here we review the current evidence on the dopaminergic system in human peripheral blood lymphocytes (PBL). PBL synthesize DA through the tyrosine-hydroxylase/DOPA-decarboxylase pathway, and express DA receptors and DA transporter (DAT) on their plasma membrane. Stimulation of DA receptors on PBL membrane contributes to modulate the development and initiation of immune responses under physiolog...

  10. Blood Clots

    Science.gov (United States)

    ... or prevent blood clots from dissolving properly. Risk factors for excessive blood clotting include Certain genetic disorders Atherosclerosis Diabetes Atrial fibrillation Overweight, obesity, and metabolic syndrome Some medicines Smoking deep vein ...

  11. Blood Transfusion

    Science.gov (United States)

    ... to their work or home. The availability of plastic bags that can have one or more satellite bags ... in preparing the donated blood. The use of plastic bags allows the blood center to make a variety ...

  12. Surface modification of polypropylene nonwovens with LDV peptidomimetics and their application in the leukodepletion of blood products.

    Science.gov (United States)

    Gérard, Estelle; Bessy, Emilie; Hénard, Grégory; Verpoort, Thierry; Marchand-Brynaert, Jacqueline

    2012-08-01

    For clinical indications and according to European standards, a depletion of the leukocytes from whole blood has to be realized before transfusion to a patient. In this study, the surface of a layer of meltblown oxygen-plasma treated polypropylene nonwoven (O(2)-PP), making part of the composition of leukodepletion filters, was modified with bioactive molecules to enhance the adhesion of leukocytes. These synthetic small molecules (called peptidomimetics) mimic the "Leu-Asp-Val" (LDV) tripeptide sequence recognized by the α(4)β(1) integrin, a receptor expressed on leukocytes. Their activity, as ligands of the α(4)β(1) integrin, was confirmed through cell adhesion assays. The peptidomimetics were covalently immobilized on O(2)-PP nonwoven via activation of the hydroxyl- and carboxyl-functions displayed on the polymer surface with trifluoro-triazine reagent, or were simply deposited on the O(2)-PP surface. The treated materials were characterized by X-ray photoelectron spectroscopy, wettability, and morphological analyses, before and after steam sterilization. Experimental filters composed of 10 layers of O(2)-PP nonwovens and a last layer modified with the peptidomimetics were evaluated, using whole blood filtration assays, for their leukodepletion efficiency, the recovery of red cells and platelets and the waste of blood, with an objective to design new filters fulfilling practical and medical criteria.

  13. Approval plans issues and innovations

    CERN Document Server

    Katz, Linda S

    2013-01-01

    How can you, as an acquisition librarians, keep current on the output of hundreds of publishers? The answer, of course, is that you cannot. For over 30 years, approval plans have been used by librarians to acquire current titles, save staff time, and build core collections. Even today, these reasons seem appropriate, as libraries try to maintain up-to-date collections and control personnel and operating budgets. However, as shown in Approval Plans: Issues and Innovations, the use of approval plans is not so simple and straightforward; their use is subject to complex procedures and policies--an

  14. The application of Reiki in nurses diagnosed with Burnout Syndrome has beneficial effects on concentration of salivary IgA and blood pressure.

    Science.gov (United States)

    Díaz-Rodríguez, Lourdes; Arroyo-Morales, Manuel; Cantarero-Villanueva, Irene; Férnandez-Lao, Carolina; Polley, Marie; Fernández-de-las-Peñas, César

    2011-01-01

    This study aimed to investigate the immediate effects of the secretory immunoglobulin A (sIgA), α-amylase activity and blood pressure levels after the application of a Reiki session in nurses with Burnout Syndrome. A randomized, double-blind, placebo-controlled, crossover design was conducted to compare the immediate effects of Reiki versus control intervention (Hand-off sham intervention) in nurses with Burnout Syndrome. Sample was composed of eighteen nurses (aged 34-56 years) with burnout syndrome. Participants were randomly assigned to receive either a Reiki treatment or a placebo (sham Reiki) treatment, according to the established order in two different days. The ANOVA showed a significant interaction time x intervention for diastolic blood pressure (F=4.92, P=0.04) and sIgA concentration (F=4.71, P=0.04). A Reiki session can produce an immediate and statistically significant improvement in sIgA concentration and diastolic blood pressure in nurses with Burnout Syndrome.

  15. A systems biology approach to the blood-aluminium problem: the application and testing of a computational model.

    Science.gov (United States)

    Beardmore, James; Rugg, Gordon; Exley, Christopher

    2007-09-01

    Transport and distribution of systemic aluminium are influenced by its interaction with blood. Current understanding is centred upon the role played by the iron transport protein transferrin which has been shown to bind up to 90% of serum total aluminium. We have coined what we have called the blood-aluminium problem which states that the proportion of serum aluminium which, at any one moment in time, is bound by transferrin is more heavily influenced by kinetic constraints than thermodynamic equilibria with the result that the role played by transferrin in the transport and distribution of aluminium is likely to have been over estimated. To begin to solve the blood-aluminium problem and therewith provide a numerical solution to the aforementioned kinetic constraints we have applied and tested a simple computational model of the time-dependency of a putative transferrin ligand (L) binding aluminium to form an Al-L complex with a probability of existence, K(E), between 0% (no complex) and 100% (complex will not dissociate). The model is based upon the principles of a lattice-gas automaton which when ran for K(E) in the range 0.1-98.0% demonstrated the emergence of complex behaviour which could be defined in the terms of a set of parameters (equilibrium value, E(V), equilibrium time, E(T), peak value, P(V), peak time, P(T), area under curve, AUC) the values of which varied in a predictable way with K(E). When K(E) was set to 98% the model predicted that ca. 90% of the total aluminium would be bound by transferrin within ca. 350 simulation timesteps. We have used a systems biology approach to develop a simple model of the time-dependency of the binding of aluminium by transferrin. To use this approach to begin to solve the blood-aluminium problem we shall need to increase the complexity of the model to better reflect the heterogeneity of a biological system such as the blood.

  16. Streamlining of building permit approval processing of town and country planning department in Ghana

    Directory of Open Access Journals (Sweden)

    Noriss Kweku Hammah

    2015-12-01

    Full Text Available To date, the Ghanaian development control system has proven unsuccessful in delivering quality planning application approvals in a timely manner and it is doubtful whether it is possible to achieve that basic goal in its current form. Planning application approval assessment is performed conjointly by various planning organizations spearheaded by Accra Town and Country Planning Department (TCPD. The success in delivering quality approvals in a timely manner therefore depends on the inter-organizational task interdependency, collaboration, and teamwork of the various planning agencies that form the Accra Metropolitan Assembly. The paper puts into perspective the TCPD organizational workflow and knowledge flow based on their strategies, size, leadership style, organizational complexities, and their competencies to contribute to the success of the approval process. Further, this paper examines the reasons behind the increasing amorphous planning and unapproved development/construction and identifies the various planning approval problems. Finally, it offers modalities to curtail planning approval delays.

  17. Drugs Approved for Liver Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for liver cancer. The list includes generic names and brand names. The drug names link to NCI’s Cancer Drug Information summaries.

  18. Drugs Approved for Vulvar Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for vulvar cancer. The list includes generic names and brand names. The drug names link to NCI's Cancer Drug Information summaries.

  19. Drugs Approved for Esophageal Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for esophageal cancer. The list includes generic names and brand names. The drug names link to NCI's Cancer Drug Information summaries.

  20. Drugs Approved for Vaginal Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) to prevent vaginal cancer. The list includes generic names and brand names. The drug names link to NCI’s Cancer Drug Information summaries.

  1. Drugs Approved for Endometrial Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for endometrial cancer. The list includes generic names and brand names. The drug names link to NCI's Cancer Drug Information summaries.

  2. Drugs Approved for Penile Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for penile cancer. The list includes generic names and brand names. The drug names link to NCI’s Cancer Drug Information summaries.

  3. Is It Really FDA Approved?

    Science.gov (United States)

    ... does not approve cosmetics. Examples of cosmetics are perfumes, makeup, moisturizers, shampoos, hair dyes, face and body ... and provide the agency with a notification before marketing a new formula. FDA conducts yearly inspections of ...

  4. Electronic Voucher Approval - Financial Management

    Data.gov (United States)

    US Agency for International Development — This process provides a workflow and eSignature capability which allows the CFO to router vouchers for review and electronic signature approval to COTRs in AIDW. It...

  5. Drugs Approved for Bladder Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for bladder cancer. The list includes generic names, brand names, and common drug combinations, which are shown in capital letters.

  6. Drugs Approved for Skin Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for skin cancer. The list includes generic names and brand names. The drug names link to NCI's Cancer Drug Information summaries.

  7. Drugs Approved for Breast Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for breast cancer. The list includes generic names, brand names, and common drug combinations, which are shown in capital letters.

  8. Drugs Approved for Kaposi Sarcoma

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for Kaposi sarcoma. The list includes generic names and brand names. The drug names link to NCI's Cancer Drug Information summaries.

  9. Drugs Approved for Pancreatic Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for pancreatic cancer. The list includes generic names, brand names, and common drug combinations, which are shown in capital letters.

  10. Drugs Approved for Bone Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for bone cancer. The list includes generic names and brand names. The drug names link to NCI's Cancer Drug Information summaries.

  11. Drugs Approved for Prostate Cancer

    Science.gov (United States)

    ... 2015 2014 2013 2012 Media Resources Media Contacts Multicultural ... This page lists cancer drugs approved by the Food and Drug Administration (FDA) for prostate cancer. The list includes generic ...

  12. Trulance Approved for Chronic Constipation

    Science.gov (United States)

    ... news/fullstory_163171.html Trulance Approved for Chronic Constipation Drug designed to stimulate upper gastrointestinal tract To ... U.S. Food and Drug Administration to treat persistent constipation of unknown (idiopathic) cause in adults. Some 42 ...

  13. Blood Facts and Statistics

    Science.gov (United States)

    ... Facts and Statistics Printable Version Blood Facts and Statistics Facts about blood needs Facts about the blood ... to Top Learn About Blood Blood Facts and Statistics Blood Components Whole Blood and Red Blood Cells ...

  14. Application of Micropore Filter Technology: Exploring the Blood Flow Path in Arterial-Line Filters and Its Effect on Bubble Trapping Functions.

    Science.gov (United States)

    Herbst, Daniel P

    2017-03-01

    Conventional arterial-line filters commonly use a large volume circular shaped housing, a wetted micropore screen, and a purge port to trap, separate, and remove gas bubbles from extracorporeal blood flow. Focusing on the bubble trapping function, this work attempts to explore how the filter housing shape and its resulting blood flow path affect the clinical application of arterial-line filters in terms of gross air handling. A video camera was used in a wet-lab setting to record observations made during gross air-bolus injections in three different radially designed filters using a 30-70% glycerol-saline mixture flowing at 4.5 L/min. Two of the filters both had inlet ports attached near the filter-housing top with bottom oriented outlet ports at the bottom, whereas the third filter had its inlet and outlet ports both located at the bottom of the filter housing. The two filters with top-in bottom-out fluid paths were shown to direct the incoming flow downward as it passed through the filter, placing the forces of buoyancy and viscous drag in opposition to each other. This contrasted with the third filter's bottom-in bottom-out fluid path, which was shown to direct the incoming flow upward so that the forces of buoyancy and viscous drag work together. The direction of the blood flow path through a filter may be important to the application of arterial-line filter technology as it helps determine how the forces of buoyancy and flow are aligned with one another.

  15. Short-term application of low-dose growth hormone in surgical patients: Effects on nitrogen balance and blood glucose

    Institute of Scientific and Technical Information of China (English)

    Ming-Ming Zhang; Xiao-Ting Wu; Yong Zhou; Kun Qian; Ya-Min Zheng

    2007-01-01

    AIM: To investigate the effectiveness and safety of recombinant human growth hormone (rhGH) in postoperative patients.METHODS: A total of 48 consecutive patients undergoing abdominal operations were randomized to receive either subcutaneous rhGH (0.15 IU/kg) or placebo (menstruum) injections daily for 7 d after surgery. The two groups had similar nutritional intake. Blood samples for serum fibronectin, albumin, prealbumin, transferrin and the total lymphocyte count, as well as glucose levels were collected to study the rhGH effect. Basal laboratory evaluation, and nutritional status were estimated on d 1before as baseline and d 3 and 10 after operation using standard laboratory techniques. Nitrogen balance was measured from d 3 to 9 after operation.RESULTS: The cumulative nitrogen balance was significantly improved in rhGH group compared with the placebo group (11.37±16.82 vs -9.11±17.52, P = 0.0003).Serum fibronectin was also significantly higher in the rhGH group than in the placebo group (104.77±19.94vs 93.03±16.03, P<0.05), whereas changes in serum albumin, prealbumin, transferrin and total lymphocyte counts were not statistically significant. Mean blood glucose levels were significantly higher in the rhGH group from d 3 to 6 after operation.CONCLUSION: If blood glucose can be controlled, lowdose growth hormone together with hypocaloric nutrition is effective on improving positive nitrogen balance and protein conservation and safe is in postoperative patients.

  16. Simulation of balloon angioplasty in residually stressed blood vessels-Application of a gradient-enhanced fibre damage model.

    Science.gov (United States)

    Polindara, César; Waffenschmidt, Tobias; Menzel, Andreas

    2016-08-16

    In this contribution we study the balloon angioplasty in a residually stressed artery by means of a non-local gradient-enhanced fibre damage model. The balloon angioplasty is a common surgical intervention used to extend or reopen narrowed blood vessels in order to restore the continuous blood flow in, for instance, atherosclerotic arteries. Inelastic, i.e. predominantly damage-related and elastoplastic processes are induced in the artery during its inflation resulting in an irreversible deformation. As a beneficial consequence, provided that the inelastic deformations do not exceed a specific limit, higher deformations can be obtained within the same pressure level and a continuous blood flow can be guaranteed. In order to study the mechanical response of the artery in this scenario, we make use of the non-local gradient-enhanced model proposed in Waffenschmidt et al. (2014). In this contribution, we extend this model to make use of an incompressible format in connection with a Q1Q1P0 finite element implementation. The residual stresses in the artery are also taken into account following the framework presented in Waffenschmidt (2015). From the results it becomes apparent that, when the artery is subjected to radial stresses beyond the physiological range, damage evolution is triggered in the collagen fibres. The impact of the residual stresses on the structural response and on the circumferential stress distribution along the thickness of the arterial wall is also studied. It is observed that the residual stresses have a beneficial effect on the mechanical response of the arterial wall.

  17. Development and application of resistive pulse spectroscopy: studies on the size, form and deformability of red blood cells

    Energy Technology Data Exchange (ETDEWEB)

    Yee, J.P.

    1979-01-01

    The following studies were conducted using the resistive pulse spectroscopy (RPS) technique: cumulative spectra and individual pulse forms for rigid latex polymer spheres; acquisition and analysis of RPS spectral data by means of special computer program; interaction of red blood cells with glutaraldehyde; membrane properties of erythrocytes undergoing abrupt osmotic hemolysis; reversible effects of the binding of chlorpromazine HCl at the red cell membrane surface; effects of high cholesterol diet on erythrocytes of guinea pigs; and multi-population analysis for a mixture of fetal and maternal red cells. (HLW)

  18. In Vivo Monitoring of Hemodynamic Changes during Clogging and Unclogging of Blood Supply for the Application of Clinical Shock Detection

    Science.gov (United States)

    Kanawade, Rajesh; Stelzle, Florian; Schmidt, Michael

    This paper presents a novel methodology in early detection of clinical shock by monitoring hemodynamic changes using diffuse reflectance measurement technique. Detailed prototype of the reflectance measurement system and data analysis technique of hemodynamic monitoring was carried out in our laboratory. The real time in-vivo measurements were done from the index finger. This study demonstrates preliminary results of real time monitoring of reduced/- oxyhemoglobin changes during clogging and unclogging of blood flow in the finger tip. The obtained results were verified with pulse-oximeter values, connected to the tip of the same index finger.

  19. 双侧双瓶血培养在临床应用的初步研究%Clinical application of bilateral double bottles in blood culture

    Institute of Scientific and Technical Information of China (English)

    赵旺胜; 王珏; 文怡; 刘根焰; 顾兵; 王芳; 陈友华; 梅亚宁

    2012-01-01

    目的 探讨双侧双瓶血培养在临床应用的必要性.方法 采用全自动血培养仪对2010年4月~ 2011年4月南京医科大学第一附属医院血液科送检的693份血液标本进行培养,对双侧双瓶、单侧双瓶和单侧单瓶血培养的真阳性率和污染率分别进行统计学分析.结果 308份双侧双瓶标本中,有44份培养出细菌,其中37份为有意义阳性标本,真阳性率为12.0%(37/308),污染率为2.3%(7/308);320份单侧双瓶标本中,阳性标本31份,有意义的阳性标本23份,真阳性率为7.2%(23/320),污染率为2.5%(8/320);65份单侧单瓶标本中,有7份细菌生长,其中4份为凝固酶阴性葡萄球菌生长,真阳性率为4.6%(3/65),污染率为6.2%(4/65).双侧双瓶血培养真阳性率明显高于单侧双瓶(x2=4.051,P<0.05)及单侧单瓶,但与后者比较无统计学意义.3种培养方法污染率比较差异无统计学意义(P>0.05).结论 双侧双瓶血培养可提高血培养阳性率,且并不增加污染率.推广双侧双瓶血培养在临床应用有一定价值.%Objective To evaluate the value of bilateral double bottles for blood culture in clinical application. Methods A total of 693 blood samples obtained from the department of hematology of the First Affiliated Hospital of Nanjing Medical University from April 2010 to April 2011 were detected by automated blood culture system. The true-positive rate and the pollution rate were analyzed with 3 blood culture model, I. E. , bilateral double bottles, unilateral double bottles and unilateral single bottle. Results In 308 samples of bilateral double bottles blood culture, bacterium grew in 44 samples. The number of significant positive samples was 37, and the true-positive rate was 12. 0% (37/308) , and the pollution rate was 2. 3% (7/308 ) . In 320 samples of unilateral double bottles blood culture, bacterium grew in 31 samples. The number of significant positive samples was 23, the true-positive rate was 7

  20. Cord Blood

    Directory of Open Access Journals (Sweden)

    Saeed Abroun

    2014-05-01

    Full Text Available   Stem cells are naïve or master cells. This means they can transform into special 200 cell types as needed by body, and each of these cells has just one function. Stem cells are found in many parts of the human body, although some sources have richer concentrations than others. Some excellent sources of stem cells, such as bone marrow, peripheral blood, cord blood, other tissue stem cells and human embryos, which last one are controversial and their use can be illegal in some countries. Cord blood is a sample of blood taken from a newborn baby's umbilical cord. It is a rich source of stem cells, umbilical cord blood and tissue are collected from material that normally has no use following a child’s birth. Umbilical cord blood and tissue cells are rich sources of stem cells, which have been used in the treatment of over 80 diseases including leukemia, lymphoma and anemia as bone marrow stem cell potency.  The most common disease category has been leukemia. The next largest group is inherited diseases. Patients with lymphoma, myelodysplasia and severe aplastic anemia have also been successfully transplanted with cord blood. Cord blood is obtained by syringing out the placenta through the umbilical cord at the time of childbirth, after the cord has been detached from the newborn. Collecting stem cells from umbilical blood and tissue is ethical, pain-free, safe and simple. When they are needed to treat your child later in life, there will be no rejection or incompatibility issues, as the procedure will be using their own cells. In contrast, stem cells from donors do have these potential problems. By consider about cord blood potency, cord blood banks (familial or public were established. In IRAN, four cord blood banks has activity, Shariati BMT center cord blood bank, Royan familial cord blood banks, Royan public cord blood banks and Iranian Blood Transfusion Organ cord blood banks. Despite 50,000 sample which storage in these banks, but the

  1. Oxytocin determination by radioimmunoassay. III. Improvement to subpicogram sensitivity and application to blood levels in cyclic cattle

    Energy Technology Data Exchange (ETDEWEB)

    Schams, D. (Insitut fuer Physiologie der Sueddeutschen Versuchs- und Forschungsanstalt der Technischen Universitaet Muenchen, FRG)

    1983-01-01

    An improved RIA for measurement of oxytocin in blood is described by using an extraction method with SEP-PAK C/sub 18/ cartridges, which allows also concentration of the sample, a new antiserum with a higher sensitivity to standard oxytocin and preparation of the standard curve in buffer. The lower limit of assay sensitivity was 0.25 pg/tube, corresponding to 0.25-1.0 pg/ml plasma depending on the amount of plasma extracted. Hence, it was no problem to measure oxytocin basal concentrations in peripheral blood in the range of 0.6-4 pg/ml plasma depending on the stage of the oestrous cycle. The highest oxytocin concentrations occurred during the early and mid-luteal phase. The method has been applied also for samples from women, sheep, pigs and horses. Mean (+-SD) recovery of oxytocin added to plasma or only buffer after extraction was 71.3+-8,1%, and the coefficient of variation (CV) = 11.4% (n = 27 assays). The intra-assay CV of two control samples was 7.9+-2.8 and 7.8+-2.4% (n = 17 assays). The inter-assay CV of 5 control samples with low and high oxytocin concentrations varied between 10.8+-17.3% (n = 25 assays). The 50% intercept was 2.5+-0.3 pg, CV = 11.3% (n = 29 assays).

  2. 9 CFR 147.52 - Approved tests.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Approved tests. 147.52 Section 147.52... Approved Tests § 147.52 Approved tests. (a) The procedures for the bacteriological examination of poultry and poultry environments described in this part are approved tests for use in the NPIP. In...

  3. The dilemma of approving antidotes.

    Science.gov (United States)

    Steffen, Christian

    2007-04-20

    Clinical trials with antidotes are difficult to perform for a variety of practical, ethical, and financial reasons. As acute poisoning is a rare event, the commercial interest in basic and clinical research is low. Poisoned patients are usually not available for normal clinical trial procedures and, if they are, they cannot give informed consent. This situation results in a dilemma: antidotes are essential drugs. A resolution of the Council of Europe requests to guarantee the optimal availability of antidotes and the improvement of their use. As comprehensive data on the efficacy of antidotes are often missing, a marketing authorisation under exceptional circumstances according to Article 14(8) of Regulation (EC) No. 276/2004, will often be the only way to get an approval, as: (1) the indications for which the product in question is intended are encountered so rarely that the applicant cannot reasonably be expected to provide comprehensive evidence ("orphan drug"), (2) in the present state of scientific knowledge, comprehensive information cannot be provided, or (3) it would be contrary to generally accepted principles of medical ethics to collect such data. Typically, data on antidotes are obtained from a patchwork of studies with animals, human tissue and a few observations from human poisoning corroborated with data from clinical observations and biochemistry. Generalisations from chemical and mechanistic similarities between groups of poisons are usual, but often lack scientific evidence. Current standards of good clinical practice can rarely be observed. Therefore, public funding and other financial support are necessary incentives to initiate trials in this important area.

  4. In vitro and in vivo study of hazardous effects of Ag nanoparticles and Arginine-treated multi walled carbon nanotubes on blood cells: application in hemodialysis membranes.

    Science.gov (United States)

    Zare-Zardini, Hadi; Amiri, Ahmad; Shanbedi, Mehdi; Taheri-Kafrani, Asghar; Kazi, S N; Chew, B T; Razmjou, Amir

    2015-09-01

    One of the novel applications of the nanostructures is the modification and development of membranes for hemocompatibility of hemodialysis. The toxicity and hemocompatibility of Ag nanoparticles and arginine-treated multiwalled carbon nanotubes (MWNT-Arg) and possibility of their application in membrane technology are investigated here. MWNT-Arg is prepared by amidation reactions, followed by characterization by FTIR spectroscopy, Raman spectroscopy, and thermogravimetric analysis. The results showed a good hemocompatibility and the hemolytic rates in the presence of both MWNT-Arg and Ag nanoparticles. The hemolytic rate of Ag nanoparticles was lower than that of MWNT-Arg. In vivo study revealed that Ag nanoparticle and MWNT-Arg decreased Hematocrit and mean number of red blood cells (RBC) statistically at concentration of 100 µg mL(-1) . The mean decrease of RBC and Hematocrit for Ag nanoparticles (18% for Hematocrit and 5.8 × 1,000,000/µL) was more than MWNT-Arg (20% for Hematocrit and 6 × 1000000/µL). In addition, MWNT-Arg and Ag nanoparticles had a direct influence on the White Blood Cell (WBC) drop. Regarding both nanostructures, although the number of WBC increased in initial concentration, it decreased significantly at the concentration of 100 µg mL(-1) . It is worth mentioning that the toxicity of Ag nanoparticle on WBC was higher than that of MWNT-Arg. Because of potent antimicrobial activity and relative hemocompatibility, MWNT-Arg could be considered as a new candidate for biomedical applications in the future especially for hemodialysis membranes.

  5. Military walking blood bank and the civilian blood service.

    Science.gov (United States)

    Berséus, Olle; Hervig, Tor; Seghatchian, Jerard

    2012-06-01

    In most countries whole blood transfusions have been replaced by component therapy. This has allowed for both better usage of the blood donations and better quality during storage. While this strategy was initially motivated by the commercial need for plasma the plasma reduction also reduced the levels of low grade proteases and sialidase, hence minimizing the cellular storage lesion/microvesiculation during prolonged storage. Plasma reduction also reduces transfusion reactions associated with plasma. During special military conditions, however, blood transfusion is urgently needed without corresponding access to blood components, in particular platelets. Accordingly, new focus on whole blood has aroused and added a new challenge to the blood transfusion services. This special issue of "what is happening" highlights the planed efforts by Swedish and Norwegian groups in the developments of military walking blood bank, which is applicable to civil blood services.

  6. Electroosmotic oscillatory flow of micropolar fluid in microchannels:application to dynamics of blood flow in microfluidic devices

    Institute of Scientific and Technical Information of China (English)

    JC MISRA; S CHANDRA; GC SHIT; PK KUNDU

    2014-01-01

    The electroosmotic flow of a micropolar fluid in a microchannel bounded by two parallel porous plates undergoing periodic vibration is studied. The equations for conservation of linear and angular momentums and Gauss’s law of charge distribution are solved within the framework of the Debye-H¨uckel approximation. The fluid velocity and microrotation are assumed to depend linearly on the Reynolds number. The study shows that the amplitude of microrotation is highly sensitive to the changes in the magnitude of the suction velocity and the width of the microchannel. An increase in the micropolar parameter gives rise to a decrease in the amplitude of microrotation. Numerical estimates reveal that the microrotation of the suspended microelements in blood also plays an important role in controlling the electro-osmotically actuated flow dynamics in micro-bio-fluidic devices.

  7. Application of a radiotelemetry system for chronic measurement of blood pressure, heart rate, EEG, and activity in the chicken.

    Science.gov (United States)

    Savory, C J; Kostal, L

    1997-06-01

    This paper reports the first successful chronic monitoring (for 30 days) of blood pressure, heart rate, EEG, and physical activity in a freely moving bird, following (described) implantation of a commercially available (Data Sciences International) radiotelemetry device in a 1.6-kg broiler chicken. The tip of the device's pressure sensing catheter was introduced into the descending aorta via a leg (ischiadic) artery and, although the catheter was tied in place, circulation in the leg was maintained and leg function was not impaired. EEG was recorded from the device's paired sensing electrodes positioned on the surface of the telencephalon. Physiological and activity data collected by the radiotelemetry system over 2 complete 24-h periods, 1 and 4 weeks after implantation of the device, were analyzed with the system's own (Dataquest LabPRO) analysis software. The results presented are discussed mainly in terms of variation between light and dark periods.

  8. Cell-penetrating peptides meditated encapsulation of protein therapeutics into intact red blood cells and its application.

    Science.gov (United States)

    He, Huining; Ye, Junxiao; Wang, Yinsong; Liu, Quan; Chung, Hee Sun; Kwon, Young Min; Shin, Meong Cheol; Lee, Kyuri; Yang, Victor C

    2014-02-28

    Red blood cells (RBCs) based drug carrier appears to be the most appealing for protein drugs due to their unmatched biocompatability, biodegradability, and long lifespan in the circulation. Numerous methods for encapsulating protein drugs into RBCs were developed, however, most of them induce partial disruption of the cell membrane, resulting in irreversible alterations in both physical and chemical properties of RBCs. Herein, we introduce a novel method for encapsulating proteins into intact RBCs, which was meditated by a cell penetrating peptide (CPP) developed in our lab-low molecular weight protamine (LMWP). l-asparaginase, one of the primary drugs used in treatment of acute lymphoblastic leukemia (ALL), was chosen as a model protein to illustrate the encapsulation into erythrocytes mediated by CPPs. In addition current treatment of ALL using different l-asparaginase delivery and encapsulation methods as well as their associated problems were also reviewed.

  9. An improved LC-MS/MS method for quantitation of indapamide in whole blood: application for a bioequivalence study.

    Science.gov (United States)

    Pinto, Guilherme Araújo; Pastre, Kátia Isabel Fercondini; Bellorio, Karini Bruno; de Souza Teixeira, Leonardo; de Souza, Weidson Carlo; de Abreu, Fernanda Crunivel; de Santana E Silva Cardoso, Fabiana Fernandes; Pianetti, Gerson Antônio; César, Isabela Costa

    2014-09-01

    An improved LC-MS/MS method for the quantitation of indapamide in human whole blood was developed and validated. Indapamide-d3 was used as internal standard (IS) and liquid-liquid extraction was employed for sample preparation. LC separation was performed on Synergi Polar RP-column (50 × 4.6 mm i.d.; 4 µm) and mobile phase composed of methanol and 5 mm aqueous ammonium acetate containing 1 mm formic acid (60:40), at flow rate of 1 mL/min. The run time was 3.0 min and the injection volume was 20 μL. Mass spectrometric detection was performed using electrospray ion source in negative ionization mode, using the transitions m/z 364.0 → m/z 188.9 and m/z 367.0 → m/z 188.9 for indapamide and IS, respectively. Calibration curve was constructed over the range 0.25-50 ng/mL. The method was precise and accurate, and provided recovery rates >80% for indapamide and IS. The method was applied to determine blood concentrations of indapamide in a bioequivalence study with two sustained release tablet formulations. The 90% confidence interval for the geometric mean ratios for maximum concentration was 95.78% and for the area under the concentration-time curve it was 97.91%. The tested indapamide tablets (Eurofarma Laboratórios S.A.) were bioequivalent to Natrilix®, according to the rate and extent of absorption.

  10. Immunoelectrophoresis - blood

    Science.gov (United States)

    IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum immunoglobulin electrophoresis; Amyloidosis - electrophoresis serum; Multiple myeloma - serum electrophoresis; Waldenström - serum electrophoresis

  11. Discrimination of approved drugs from experimental drugs by learning methods

    Directory of Open Access Journals (Sweden)

    Li Yixue

    2011-05-01

    Full Text Available Abstract Background To assess whether a compound is druglike or not as early as possible is always critical in drug discovery process. There have been many efforts made to create sets of 'rules' or 'filters' which, it is hoped, will help chemists to identify 'drug-like' molecules from 'non-drug' molecules. However, among the chemical space of the druglike molecules, the minority will be approved drugs. Classifying approved drugs from experimental drugs may be more helpful to obtain future approved drugs. Therefore, discrimination of approved drugs from experimental ones has been done in this paper by analyzing the compounds in terms of existing drugs features and machine learning methods. Results Four methodologies were compared by their performance to classify approved drugs from experimental ones. The best results were obtained by SVM, in which the accuracy is 0.7911, the sensitivity is 0.5929, and the specificity is 0.8743. Based on the results, consensus model was developed to effectively discriminate drugs, which further pushed the correct classification rate up to 0.8517, sensitivity up to 0.7242, specificity up to 0.9352. The applications on the Traditional Chinese Medicine Ingredients Database (TCM-ID tested the methods. Therefore this model has been proven to be a potent tool for identifying drug molecules. Conclusion The studies would have potential applications in the research of combinatorial library design and virtual high throughput screening for drug discovery.

  12. Design and Application of Clinical Blood Transfusion Management Information System%临床输血业务管理信息系统的设计与应用

    Institute of Scientific and Technical Information of China (English)

    陈兰; 周湘江; 罗安燕; 彭建

    2014-01-01

    In this paper, a blood transfusion service management subsystem is designed on the basis of the original blood bank management system to realize a integrated management from the blood bank to the clinic. The establishment and application of the management platform achieves informatization and standardization of the whole process of blood transfusion, thus making blood transfusion more effective and reasonable.%本研究在原有的血库管理系统的基础上,设计了输血业务管理信息子系统,以实现血库到临床的业务集成管理。该平台,实现了输血业务全过程的信息化、标准化管理,从而提高了科学用血水平。

  13. 13 CFR 108.380 - Final approval as a NMVC Company.

    Science.gov (United States)

    2010-01-01

    ... VENTURE CAPITAL (âNMVCâ) PROGRAM Evaluation and Selection of NMVC Companies § 108.380 Final approval as a... amount of Regulatory Capital set forth in its application, pursuant to § 108.310(a)(1); and (B) The... at least 30 percent of its Regulatory Capital if the Conditionally Approved NMVC Company— (i)...

  14. 77 FR 74225 - Proposed Extension of the Approval of Information Collection Requirements

    Science.gov (United States)

    2012-12-13

    ... Division Administrator an application for approval of a Work Study Program as one that does not interfere... and Hour Division. Title: Work-Study Program of the Child Labor Regulations (WSP) Regulations 29 CFR... Hour Division RIN 1235-0024 Proposed Extension of the Approval of Information Collection...

  15. Clinical Application of Blood Concentration Monitoring of Vancomycin%万古霉素血药浓度监测的临床应用

    Institute of Scientific and Technical Information of China (English)

    任婷麟; 于春令

    2012-01-01

    Objective To investigate the clinical application of blood concentration monitoring to guide rational use of vancomycin. Methods The results of blood concentration monitoring of 79 patients with vancomycin use from Jul. 2009 to Sept. 2011 were analyzed retrospectively. Results The total response rate of vancomycin was 61. 76% . The concentrations of 22. 78% of the cases was 5-10 mg/L, that of5.06% was below 5 mg/L and that of 34.18% was over 20 mg/L. Conclusion It is important to formulate individualized dosing regimens based on the blood vancomycin level, which can ensure clinical efficacy and reduce incidence of toxicities.%目的 探讨万古霉素血药浓度监测的临床应用,以指导临床合理用药.方法 对海军总医院2009年7月~2011年9月住院患者使用万古霉素的79例次血药浓度监测结果进行回顾性分析.结果 该院住院患者使用万古霉素的总有效率为61.76%.血液中药物谷浓度在5~10 mg/L的占22.78%;小于5 mg/L有4例次,占5.06%;大于20mg/L有27例次,占34.18%.结论 根据血药浓度制定个体化给药方案,能提高疗效,减少不良反应.

  16. 46 CFR 160.077-6 - Approval procedures.

    Science.gov (United States)

    2010-10-01

    ... PFD of sufficiently similar design. (2) Engineering analysis showing that the test is not applicable...) Alternative Requirements. A PFD that does not meet requirements in this subpart may still be approved if the device— (1) Meets other requirements prescribed by the Commandant in place of or in addition...

  17. Understanding Blood Counts

    Science.gov (United States)

    ... Lab and Imaging Tests Understanding Blood Counts Understanding Blood Counts Understanding Blood Counts SHARE: Print Glossary Blood cell counts give ... your blood that's occupied by red cells. Normal Blood Counts Normal blood counts fall within a range ...

  18. Gliadin-Specific T-Cells Mobilized in the Peripheral Blood of Coeliac Patients by Short Oral Gluten Challenge: Clinical Applications.

    Science.gov (United States)

    Picascia, Stefania; Mandile, Roberta; Auricchio, Renata; Troncone, Riccardo; Gianfrani, Carmen

    2015-12-02

    Celiac disease (CD) is a common lifelong food intolerance triggered by dietary gluten affecting 1% of the general population. Gliadin-specific T-cell lines and T-cell clones obtained from intestinal biopsies have provided great support in the investigation of immuno-pathogenesis of CD. In the early 2000 a new in vivo, less invasive, approach was established aimed to evaluate the adaptive gliadin-specific T-cell response in peripheral blood of celiac patients on a gluten free diet. In fact, it has been demonstrated that three days of ingestion of wheat-containing food induces the mobilization of memory T lymphocytes reactive against gliadin from gut-associated lymphoid tissue into peripheral blood of CD patients. Such antigen-specific T-cells releasing interferon-γ can be transiently detected by using the enzyme-linked immunospot (ELISPOT) assays or by flow cytometry tetramer technology. This paper discusses the suitability of this in vivo tool to investigate the repertoire of gluten pathogenic peptides, to support CD diagnosis, and to assess the efficacy of novel therapeutic strategies. A systematic review of all potential applications of short oral gluten challenge is provided.

  19. Gliadin-Specific T-Cells Mobilized in the Peripheral Blood of Coeliac Patients by Short Oral Gluten Challenge: Clinical Applications

    Directory of Open Access Journals (Sweden)

    Stefania Picascia

    2015-12-01

    Full Text Available Celiac disease (CD is a common lifelong food intolerance triggered by dietary gluten affecting 1% of the general population. Gliadin-specific T-cell lines and T-cell clones obtained from intestinal biopsies have provided great support in the investigation of immuno-pathogenesis of CD. In the early 2000 a new in vivo, less invasive, approach was established aimed to evaluate the adaptive gliadin-specific T-cell response in peripheral blood of celiac patients on a gluten free diet. In fact, it has been demonstrated that three days of ingestion of wheat-containing food induces the mobilization of memory T lymphocytes reactive against gliadin from gut-associated lymphoid tissue into peripheral blood of CD patients. Such antigen-specific T-cells releasing interferon-γ can be transiently detected by using the enzyme-linked immunospot (ELISPOT assays or by flow cytometry tetramer technology. This paper discusses the suitability of this in vivo tool to investigate the repertoire of gluten pathogenic peptides, to support CD diagnosis, and to assess the efficacy of novel therapeutic strategies. A systematic review of all potential applications of short oral gluten challenge is provided.

  20. Gliadin-Specific T-Cells Mobilized in the Peripheral Blood of Coeliac Patients by Short Oral Gluten Challenge: Clinical Applications

    Science.gov (United States)

    Picascia, Stefania; Mandile, Roberta; Auricchio, Renata; Troncone, Riccardo; Gianfrani, Carmen

    2015-01-01

    Celiac disease (CD) is a common lifelong food intolerance triggered by dietary gluten affecting 1% of the general population. Gliadin-specific T-cell lines and T-cell clones obtained from intestinal biopsies have provided great support in the investigation of immuno-pathogenesis of CD. In the early 2000 a new in vivo, less invasive, approach was established aimed to evaluate the adaptive gliadin-specific T-cell response in peripheral blood of celiac patients on a gluten free diet. In fact, it has been demonstrated that three days of ingestion of wheat-containing food induces the mobilization of memory T lymphocytes reactive against gliadin from gut-associated lymphoid tissue into peripheral blood of CD patients. Such antigen-specific T-cells releasing interferon-γ can be transiently detected by using the enzyme-linked immunospot (ELISPOT) assays or by flow cytometry tetramer technology. This paper discusses the suitability of this in vivo tool to investigate the repertoire of gluten pathogenic peptides, to support CD diagnosis, and to assess the efficacy of novel therapeutic strategies. A systematic review of all potential applications of short oral gluten challenge is provided. PMID:26633487

  1. BLOOD DONATION

    CERN Multimedia

    SC Unit

    2008-01-01

    A blood donation, organized by EFS (Etablissement Français du Sang) of Annemasse will take place On Wednesday 12 November 2008, from 8:30 to 16:00, at CERN Restaurant 2 If possible, please, bring your blood group Card.

  2. Blood donation

    CERN Multimedia

    GS Department

    2009-01-01

    A blood donation is organised by the Cantonal Hospital of Geneva On Thursday 19 March 2009 from 9 a.m. to 5 p.m. CERN RESTAURANT 2 Number of donations during the last blood donations :135 donors in July 2008 122 donors in November 2008 Let’s do better in 2009 !!! Give 30 minutes of your time to save lives...

  3. Development of hedge operator based fuzzy divergence measure and its application in segmentation of chronic myelogenous leukocytes from microscopic image of peripheral blood smear.

    Science.gov (United States)

    Ghosh, Madhumala; Chakraborty, Chandan; Konar, Amit; Ray, Ajoy K

    2014-02-01

    This paper introduces a hedge operator based fuzzy divergence measure and its application in segmentation of leukocytes in case of chronic myelogenous leukemia using light microscopic images of peripheral blood smears. The concept of modified discrimination measure is applied to develop the measure of divergence based on Shannon exponential entropy and Yager's measure of entropy. These two measures of divergence are compared with the existing literatures and validated by ground truth images. Finally, it is found that hedge operator based divergence measure using Yager's entropy achieves better segmentation accuracy i.e., 98.29% for normal and 98.15% for chronic myelogenous leukocytes. Furthermore, Jaccard index has been performed to compare the segmented image with ground truth ones where it is found that that the proposed scheme leads to higher Jaccard index (0.39 for normal, 0.24 for chronic myelogenous leukemia).

  4. Tainted blood

    DEFF Research Database (Denmark)

    Deleuran, Ida; Sheikh, Zainab Afshan; Hoeyer, Klaus

    2015-01-01

    study of the historical rise and current workings of safety practices in the Danish blood system. Here, we identify a strong focus on contamination in order to avoid 'tainted blood', at the expense of working with risks that could be avoided through enhanced blood monitoring practices. Of further...... significance to this focus are the social dynamics found at the heart of safety practices aimed at avoiding contamination. We argue that such dynamics need more attention, in order to achieve good health outcomes in transfusion medicine. Thus, we conclude that, to ensure continuously safe blood systems, we...... need to move beyond the bifurcation of the social and medical aspects of blood supply as two separate issues and approach social dynamics as key medical safety questions....

  5. Dynamic simulation of red blood cell metabolism and its application to the analysis of a pathological condition

    Directory of Open Access Journals (Sweden)

    Kinoshita Ayako

    2005-05-01

    Full Text Available Abstract Background Cell simulation, which aims to predict the complex and dynamic behavior of living cells, is becoming a valuable tool. In silico models of human red blood cell (RBC metabolism have been developed by several laboratories. An RBC model using the E-Cell simulation system has been developed. This prototype model consists of three major metabolic pathways, namely, the glycolytic pathway, the pentose phosphate pathway and the nucleotide metabolic pathway. Like the previous model by Joshi and Palsson, it also models physical effects such as osmotic balance. This model was used here to reconstruct the pathology arising from hereditary glucose-6-phosphate dehydrogenase (G6PD deficiency, which is the most common deficiency in human RBC. Results Since the prototype model could not reproduce the state of G6PD deficiency, the model was modified to include a pathway for de novo glutathione synthesis and a glutathione disulfide (GSSG export system. The de novo glutathione (GSH synthesis pathway was found to compensate partially for the lowered GSH concentrations resulting from G6PD deficiency, with the result that GSSG could be maintained at a very low concentration due to the active export system. Conclusion The results of the simulation were consistent with the estimated situation of real G6PD-deficient cells. These results suggest that the de novo glutathione synthesis pathway and the GSSG export system play an important role in alleviating the consequences of G6PD deficiency.

  6. The anticoagulant ability of ferulic acid and its applications for improving the blood compatibility of silk fibroin

    Energy Technology Data Exchange (ETDEWEB)

    Wang Song; Gao Zhen; Chen Xiaomeng; Lian Xiaojie; Zhu Hesun [School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081 (China); Zheng Jun; Sun Lizhong [Department of Cardiac Surgery, Cardiovascular Institute and Fu Wai Hospital, CAMS and PUMC, Beijing 100037 (China)], E-mail: wangsongbit@hotmail.com

    2008-12-15

    The hemocompatibility of silk fibroin (SF) was improved with ferulic acid (FA) by graft polymerization. Ferulic acid is an active ingredient of many Chinese herbal medicines, such as Chuanxiong (Rhizoma ligustici wallichii), Danggui (Angelica sinensis) and Awei (Asafoetida giantfennel), which have been used to treat cardiovascular diseases by Chinese physicians for thousands of years. The inhibitory functions of FA on blood coagulation and erythrocyte agglutination were first characterized by a Lee-White test tube method and a micropipette technique, respectively. Then, FA was immobilized on SF by graft polymerization and the surface composition of modified SF was characterized by attenuated total reflectance Fourier-transform infrared (ATR-FTIR), x-ray photoelectron spectroscopy (XPS) and optical microscopy. The anticoagulant activity of modified SF was assessed, respectively, by in vitro clotting time measurements on a photo-optical clot detection instrument and with the Lee-White test tube method. The test results indicated that in comparison to untreated SF, the anticoagulant activity of modified SF has been improved significantly. Moreover, the SF surface composition is altered by FA but its {beta}-sheet conformation is not disturbed.

  7. The dopaminergic system in peripheral blood lymphocytes: from physiology to pharmacology and potential applications to neuropsychiatric disorders.

    Science.gov (United States)

    Buttarelli, Francesca R; Fanciulli, Alessandra; Pellicano, Clelia; Pontieri, Francesco E

    2011-06-01

    Besides its action on the nervous system, dopamine (DA) plays a role on neural-immune interactions. Here we review the current evidence on the dopaminergic system in human peripheral blood lymphocytes (PBL). PBL synthesize DA through the tyrosine-hydroxylase/DOPA-decarboxylase pathway, and express DA receptors and DA transporter (DAT) on their plasma membrane. Stimulation of DA receptors on PBL membrane contributes to modulate the development and initiation of immune responses under physiological conditions and in immune system pathologies such as autoimmunity or immunodeficiency.The characterization of DA system in PBL gave rise to a further line of research investigating the feasibility of PBL as a cellular model for studying DA derangement in neuropsychiatric disorders. Several reports showed changes of the expression of DAT and/or DA receptors in PBL from patients suffering from several neuropsychiatric disorders, in particular parkinsonian syndromes, schizophrenia and drug- or alcohol-abuse. Despite some methodological and theoretical limitations, these findings suggest that PBL may prove a cellular tool with which to identify the derangement of DA transmission in neuropsychiatric diseases, as well as to monitor the effects of pharmacological treatments.

  8. REVIEW OF NRC APPROVED DIGITAL CONTROL SYSTEMS ANALYSIS

    Energy Technology Data Exchange (ETDEWEB)

    D.W. Markman

    1999-09-17

    Preliminary design concepts for the proposed Subsurface Repository at Yucca Mountain indicate extensive reliance on modern, computer-based, digital control technologies. The purpose of this analysis is to investigate the degree to which the U. S. Nuclear Regulatory Commission (NRC) has accepted and approved the use of digital control technology for safety-related applications within the nuclear power industry. This analysis reviews cases of existing digitally-based control systems that have been approved by the NRC. These cases can serve as precedence for using similar types of digitally-based control technologies within the Subsurface Repository. While it is anticipated that the Yucca Mountain Project (YMP) will not contain control systems as complex as those required for a nuclear power plant, the review of these existing NRC approved applications will provide the YMP with valuable insight into the NRCs review process and design expectations for safety-related digital control systems. According to the YMP Compliance Program Guidance, portions of various NUREGS, Regulatory Guidelines, and nuclear IEEE standards the nuclear power plant safety related concept would be applied to some of the designs on a case-by-case basis. This analysis will consider key design methods, capabilities, successes, and important limitations or problems of selected control systems that have been approved for use in the Nuclear Power industry. An additional purpose of this analysis is to provide background information in support of further development of design criteria for the YMP. The scope and primary objectives of this analysis are to: (1) Identify and research the extent and precedence of digital control and remotely operated systems approved by the NRC for the nuclear power industry. Help provide a basis for using and relying on digital technologies for nuclear related safety critical applications. (2) Identify the basic control architecture and methods of key digital control

  9. Study on the Application of Blood Test in Diagnosis of Anemia%血液检验在贫血诊断中的应用研究

    Institute of Scientific and Technical Information of China (English)

    孔瑞微; 赵文凤

    2015-01-01

    Objective To analyze the clinical application effect of blood test in diagnosis of anemia. Methods The patients with thalassemia and the patients with iron-deficiency anemia who were treated in hospital were selected from January 2014 to February 2015 and divided them into Group A and Group B,24 patients of each group. And 24 cases of healthy people were chose as Group C. And blood related indexes were compared of these three groups. Results The levels of MCV and MCH of Group A and Group B were quite lower than those in Group C,there was significant difference between two groups(P0.05). Conclusion Blood test is effective in diagnosis of anemia. The level of RBC,MCV,MCH,RDW,MCHC and other indicators can be used as criteria in diagnosis of anemia. Blood test is quite worthwhile to be promoted and applied in clinical diagnosis of anemia.%目的:对血液检验在贫血诊断中的临床应用效果进行分析探讨。方法选取于2014年1月~2015年2月来我院就诊的地中海贫血患者和缺铁性贫血患者各24例,分别设置为A组和B组;再选取24例健康成人设置为C组,比较三组人员血液检测的相关指标。结果A、B组的MCV、MCH均显著低于C组,差异有统计学意义(P0.05)。结论血液检测在贫血鉴别诊断中效果显著,通过对RBC、MCV、MCH、RDW、MCHC等指标能够作为贫血诊断的标准。

  10. Endovascular blood flow measurement system

    Science.gov (United States)

    Khe, A. K.; Cherevko, A. A.; Chupakhin, A. P.; Krivoshapkin, A. L.; Orlov, K. Yu

    2016-06-01

    In this paper an endovascular measurement system used for intraoperative cerebral blood flow monitoring is described. The system is based on a Volcano ComboMap Pressure and Flow System extended with analogue-to-digital converter and PC laptop. A series of measurements performed in patients with cerebrovascular pathologies allows us to introduce “velocity-pressure” and “flow rate-energy flow rate” diagrams as important characteristics of the blood flow. The measurement system presented here can be used as an additional instrument in neurosurgery for assessment and monitoring of the operation procedure. Clinical data obtained with the system are used for construction of mathematical models and patient-specific simulations. The monitoring of the blood flow parameters during endovascular interventions was approved by the Ethics Committee at the Meshalkin Novosibirsk Research Institute of Circulation Pathology and included in certain surgical protocols for pre-, intra- and postoperative examinations.

  11. What Happens to Donated Blood?

    Science.gov (United States)

    ... week. Learn About Blood Blood Facts and Statistics Blood Components Whole Blood and Red Blood Cells Platelets Plasma ... About Blood Blood Facts and Statistics Blood Types Blood Components What Happens to Donated Blood Blood and Diversity ...

  12. 血液检验在临床贫血诊断中的价值%Application Value Analysis of Blood Test in the Diagnosis of Clinical Anemia

    Institute of Scientific and Technical Information of China (English)

    陈群; 张志城; 邓宝佳; 邝紫华

    2016-01-01

    目的:探讨临床贫血诊断中血液检验的应用价值。方法:以47例地中海贫血患者、53例缺铁性贫血患者及40例健康体检者为研究对象。对比分析三组患者血液中RBC、MCH、Hb、MCV和RDW五项观察指标差异,计算血液检验方法应用于不同类型贫血诊断中检验灵敏度、特异性和约登指数,对各项数据结果予进行对比分析。结果:三组各项血液检测观察指标差异明显(P<0.05);地中海贫血组MCH和Hb水平明显高于缺铁性贫血组(P<0.05),明显低于对照组(P<0.05),而RDW指标水平明显低于缺铁性贫血组(P<0.05);缺铁性贫血组RDW水平明显高于其它两组(P<0.05)。结论:临床诊断贫血时血液检验可取得良好的诊断效果,但其误诊率也较高,辅以其它诊断方式可取得更为确切的鉴别诊断结果。%Objective To explore the application value of blood test in the diagnosis of clinical anemia.Methods 47 cases of pa-tients with the Mediterranean anemia,53 patients with iron deficiency anemia and 40 cases of healthy subjects were studied.Compared and analyzed the difference in three groups of patients with blood RBC,MCH,Hb,MCV and RDW five observation indexs,calculated the blood test method application in different types of Anemia Diagnosis mid-term test sensitivity,specificity and Youden′s finger. Results The difference of all the blood test observation indexs in the three groups was significant (P<0.05 );MCH and Hb level in mediterranean anemia group was significantly higher than that of iron deficiency anemia group (P<0.05 ),was significantly lower than that in the contrast group (P<0.05 ),and RDW levels was significantly lower than that of ischemic anemia group (P<0.05 );ischemic anemia group of RDW levels were significantly higher than those in the other two groups (P<0.05 ).Conclusion The clini-cal diagnosis of anemia in the blood test can achieve good results,but the

  13. ELECTRONIC COMPLIANCE AND APPROVAL PROJECT (ECAP)

    Energy Technology Data Exchange (ETDEWEB)

    Hope Morgan; Richard A. Varela; Deborah LaHood; Susan Cisco; Mary Ann Benavides; Donna Burks

    2002-11-01

    The Texas Railroad Commission (RRC), working in partnership with the United States Department of Energy and the oil and gas industry it regulates, is implementing a strategy for improving efficiency in regulations and significantly reducing administrative operating costs through the Electronic Compliance and Approval Process (ECAP). The project will streamline regulatory compliance and reporting by providing the ability to electronically submit, process, and query oil and gas applications and reports through the Internet-based ECAP system. Implementation of an ECAP drilling permit pilot project began September 1999 after funding resources were secured--a $700,000 grant from the U.S. Department of Energy and an appropriation of $1.4 million from the Texas Legislature. The pilot project involves creating the ability to file, review, and approve a well's drilling permit application through a completely electronic process. The pilot project solution will ultimately provide the infrastructure, technology, and electronic modules to enable the filing of all compliance permits and performance reports through the internet from a desktop computer. The pilot project was conducted in three phases. The first phase, implemented May 2000, provided the infrastructure that allows the electronic filing and approval of simple drilling permit applications, associated fees, and attachments. The official ''roll-out'' of ECAP and the first electronically filed drilling permit application occurred on May 11, 2000 in Dallas in conjunction with an Internet Workshop sponsored by the Petroleum Technology Transfer Council. After the completion of Phase I, the ECAP team conducted an extensive review of progress to date and analyzed requirements and opportunities for future steps. The technical team identified core infrastructure modifications that would facilitate and better support future development and expansion of the ECAP system and work began on database structure

  14. Application and evaluation of modified sheep blood chocolate culture medium%改良羊血巧克力培养基的应用与评价

    Institute of Scientific and Technical Information of China (English)

    傅石明; 宋月芹; 朱香梅

    2015-01-01

    目的:评价改良羊血巧克力培养基的质量与应用价值。方法将 ATCC 10211流感嗜血杆菌接种到改良前后两种培养基上,比较两种培养基上嗜血杆菌的平均生长指数(GI 值);对352份经筛选的合格痰标本进行流感嗜血杆菌检测,比较两种培养基的阳性分离率。结果传统羊血巧克力培养基 GI 值为(3.69±0.58),改良羊血巧克力培养基 GI 值为(15.08±1.34),两种培养基 GI 值差异有统计学意义(t =25.31,P <0.01)。352份痰标本在改良羊血巧克力培养基上流感嗜血杆菌检出41例,阳性检出率11.65%。352份痰标本在传统羊血巧克力培养基上流感嗜血杆菌检出18例,阳性检出率5.54%。流感嗜血杆菌在两种巧克力培养基上的分离率差异有统计学意义(χ2=21.04,P <0.05)。结论流感嗜血杆菌在改良羊血巧克力培养基上生长良好,菌落明显,极易识别,有助于临床标本中流感嗜血杆菌的检出。%Objective To evaluate the quality and application value of improved sheep blood chocolate medium.Methods The ATCC 10211 of Haemophilus influenzae was inoculated into the modified medium and unmodified medium,the average growth index(GI)of Haemophilus influenzae in two kinds of culture medium was compared.Based on 352 selected qualified sputum specimens for detection of Haemophilus influenzae,the positive isolation rate of medium was compared between the two groups.Results GI value of the traditional blood chocolate culture medium was (3.69 ±0.58),which was significantly lower than (15.08 ±1.34)of the improved sheep blood chocolate culture medium,the difference was significant (t =25.31,P <0.01 ).352 sputum specimens in the improved sheep blood chocolate culture medium,Haemophilus influenzae detected in 41 cases,the positive rate was 11.65%.And 352 sputum specimens in traditional sheep blood chocolate culture,Haemophilus influenzae

  15. Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood

    Directory of Open Access Journals (Sweden)

    Joanna Majewska

    2015-08-01

    Full Text Available A specific humoral response to bacteriophages may follow phage application for medical purposes, and it may further determine the success or failure of the approach itself. We present a long-term study of antibody induction in mice by T4 phage applied per os: 100 days of phage treatment followed by 112 days without the phage, and subsequent second application of phage up to day 240. Serum and gut antibodies (IgM, IgG, secretory IgA were analyzed in relation to microbiological status of the animals. T4 phage applied orally induced anti-phage antibodies when the exposure was long enough (IgG day 36, IgA day 79; the effect was related to high dosage. Termination of phage treatment resulted in a decrease of IgA again to insignificant levels. Second administration of phage induces secretory IgA sooner than that induced by the first administrations. Increased IgA level antagonized gut transit of active phage. Phage resistant E. coli dominated gut flora very late, on day 92. Thus, the immunological response emerges as a major factor determining phage survival in the gut. Phage proteins Hoc and gp12 were identified as highly immunogenic. A low response to exemplary foreign antigens (from Ebola virus presented on Hoc was observed, which suggests that phage platforms can be used in oral vaccine design.

  16. Drugs Approved for Hodgkin Lymphoma

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for Hodgkin lymphoma. The list includes generic names, brand names, and common drug combinations, which are shown in capital letters. The drug names link to NCI's Cancer Drug Information summaries.

  17. Drugs Approved for Multiple Myeloma

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for multiple myeloma and other plasma cell neoplasms. The list includes generic names, brand names, and common drug combinations, which are shown in capital letters. The drug names link to NCI's Cancer Drug Information summaries.

  18. Drugs Approved for Myeloproliferative Neoplasms

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for myeloproliferative neoplasms. The list includes generic names, brand names, and common drug combinations, which are shown in capital letters. The drug names link to NCI's Cancer Drug Information summaries.

  19. Alkylbenzene Project in Xinjiang Approved

    Institute of Scientific and Technical Information of China (English)

    Zhou Weiyong

    1996-01-01

    @@ The feasibility study on alkylbenzene project in Dushanzi, Xinjiang Uygur Autonomous Region, submitted jointly by the government of Xinjiang UygurAutonomous Region and China National Petroleum Corporation (CNPC), has been approved by State Council recently,after pass appraisa l by China International Engineering Consulting Corporation entrusted by State Planning Committee.

  20. Blood pressure and atherosclerosis

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    2010319 Effects of combined application of Xuezhikang capsule with hypotensive drugs on arterial compliance and smoothness of the dynamic blood pressure. ZHU Zongtao(朱宗涛),et al. Dept Cardiol, Centr People’s Hosp, Tengzhou 277500.Chin J Integr Tradit & West Med 2010;30

  1. Blood Typing

    Science.gov (United States)

    ... if you need repeated transfusions, as sickle cell anemia and thalassemia patients do. If blood transfusions are not closely ... the News Article Index About This Site Send Us Your Comments For ...

  2. Blood Disorders

    Science.gov (United States)

    ... people with blood disorders. Magnitude of the Problem Complications from deep vein thrombosis (DVT) kill more people each year than breast cancer, motor vehicle accidents, and HIV combined. Sickle cell trait ...

  3. What's Blood?

    Science.gov (United States)

    ... Rh" because scientists found it while studying Rhesus monkeys. If your blood is positive, you have this ... doctor. © 1995- The Nemours Foundation. All rights reserved. Images provided by The Nemours Foundation, iStock, Getty Images, ...

  4. Number of Drilling Permits Approved by Fiscal Year on Federal Lands by BLM

    Data.gov (United States)

    Bureau of Land Management, Department of the Interior — This table contains the total number of Applications for Permit to Drill (APDs) by state approved by the BLM each fiscal year. Oil and gas operators may not begin...

  5. 76 FR 77182 - Approval and Promulgation of Air Quality Implementation Plans; Virginia; General Conformity...

    Science.gov (United States)

    2011-12-12

    ... Conformity Requirements for Federal Agencies Applicable to Federal Actions AGENCY: Environmental Protection... adopted by Virginia for the purpose of incorporating Federal general conformity requirements revisions... approving Virginia's general conformity SIP revision and if that provision may be severed from the...

  6. 77 FR 53249 - Agency Information Collection Activities: Requests for Comments; Clearance of Renewed Approval of...

    Science.gov (United States)

    2012-08-31

    ... and Budget (OMB) approval to renew an information collection. The FAA collects information from... monitoring. Information is collected in the application, and grant agreement amendments; financial management... projects. Frequency: Information is collected on occasion. Estimated Average Burden per Response:...

  7. 76 FR 47210 - Medical Devices; Availability of Safety and Effectiveness Summaries for Premarket Approval...

    Science.gov (United States)

    2011-08-04

    .... Applicant Trade name Approval date P050050 FDA-2011-M-0323....... Small Bone Scandinavian total ankle May 27, 2009. Innovations, Inc. replacement system. P060004(S1) FDA-2011-M-0256... Carl Zeiss Meditec,...

  8. 78 FR 57213 - Notice of Passenger Facility Charge (PFC) Approvals and Disapprovals

    Science.gov (United States)

    2013-09-17

    ... of Sec. 158.29. PFC Applications Approved Public Agency: City of Savannah and Savannah Airport...: Noise compatibility program at BUF. Glycol treatment project at BUF. Runway friction tester at BUF....

  9. 75 FR 2090 - Approval and Promulgation of Air Quality Implementation Plans; Indiana; Volatile Organic Compound...

    Science.gov (United States)

    2010-01-14

    ... Organic Compound Automobile Refinishing Rules for Indiana AGENCY: Environmental Protection Agency (EPA... relations, Nitrogen dioxide, Ozone, Reporting and recordkeeping requirements, Volatile organic compounds... Plan (SIP). These rule revisions extend the applicability of Indiana's approved volatile...

  10. Modeling microbial ethanol production by E. coli under aerobic/anaerobic conditions: applicability to real postmortem cases and to postmortem blood derived microbial cultures.

    Science.gov (United States)

    Boumba, Vassiliki A; Kourkoumelis, Nikolaos; Gousia, Panagiota; Economou, Vangelis; Papadopoulou, Chrissanthy; Vougiouklakis, Theodore

    2013-10-10

    The mathematical modeling of the microbial ethanol production under strict anaerobic experimental conditions for some bacterial species has been proposed by our research group as the first approximation to the quantification of the microbial ethanol production in cases where other alcohols were produced simultaneously with ethanol. The present study aims to: (i) study the microbial ethanol production by Escherichia coli under controlled aerobic/anaerobic conditions; (ii) model the correlation between the microbial produced ethanol and the other higher alcohols; and (iii) test their applicability in: (a) real postmortem cases that had positive BACs (>0.10 g/L) and co-detection of higher alcohols and 1-butanol during the original ethanol analysis and (b) postmortem blood derived microbial cultures under aerobic/anaerobic controlled experimental conditions. The statistical evaluation of the results revealed that the formulated models were presumably correlated to 1-propanol and 1-butanol which were recognized as the most significant descriptors of the modeling process. The significance of 1-propanol and 1-butanol as descriptors was so powerful that they could be used as the only independent variables to create a simple and satisfactory model. The current models showed a potential for application to estimate microbial ethanol - within an acceptable standard error - in various tested cases where ethanol and other alcohols have been produced from different microbes.

  11. Artificial blood.

    OpenAIRE

    1983-01-01

    #Blood substitutes have been developed for almost a century. The various type of artificial blood was continuously available on the market. The theme of this report is to identify the best substitute in emergency situation for some patients and science students. The definition of best is given; thus, as the vital part of the report, the comparison between them is described and discussed. Modified hemoglobin, bovine-based hemoglobin and PFCs are three basic types. In terms of the perfor...

  12. Blood Transfusion and Donation

    Science.gov (United States)

    ... receiving the blood transfusion. To keep blood safe, blood banks carefully screen donated blood. The risk of catching ... one or more times before the surgery. A blood bank will store your blood for your use. NIH: ...

  13. Blood (For Parents)

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Blood KidsHealth > For Parents > Blood A A A What's ... about the mysterious, life-sustaining fluid called blood. Blood Basics Two types of blood vessels carry blood ...

  14. Catecholamine blood test

    Science.gov (United States)

    Norepinephrine -- blood; Epinephrine -- blood; Adrenalin -- blood; Dopamine -- blood ... A blood sample is needed. ... the test. This is especially true if both blood and urine catecholamines are to be measured. You ...

  15. Biology of Blood

    Science.gov (United States)

    ... here for the Professional Version Home Blood Disorders Biology of Blood Overview of Blood Resources In This ... Version. DOCTORS: Click here for the Professional Version Biology of Blood Overview of Blood Components of Blood ...

  16. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... Blood Basics Blood Disorders Anemia Bleeding Disorders Blood Cancers Blood Clots Blood Clotting and Pregnancy Clots and ... Increased maternal age Other medical illness (e.g., cancer, infection) back to top How are Blood Clots ...

  17. Quantitative determination of acetylshikonin in macaque monkey blood by LC-ESI-MSIMS after precolumn derivatization with 2-mercaptoethanol and its application in pharmacokinetic study

    Institute of Scientific and Technical Information of China (English)

    Dong-xiao SUN; Hui-fang TIAN; Zhi-yun MENG; Alicia DU; Dan YUAN; Ruo-lan GU; Zhuo-na WU; Gui-fang DOU

    2008-01-01

    Aim: To develop and validate a novel precolumn derivatization method for the quantitative determination and pharmaeokinetic application of acetylshikonin in macaque monkeys by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Methods: 2-Mereaptoethanol was added to the blood sample as the derivatization reagent. The derivatization reaction formed 1 major derivation product, Which was well correlated with acetyl-shikonin. The acetylshikonin concentrations in the biological samples were cal-culated by quantitative determination of the major derivation product using LC-ESI-MS/MS. Separation was achieved using a C18 column (2 mm×50 mm, 5 μm) at room temperature and a linear gradient elution with a mobile phase containing methanol (1.96% acetic acid) and 10% methanol in water (1.96% acetic acid and 10 mmol/L ammonium acetate) at a flow rate of 0.2 mL/min. In addition, the major derivative, named derivative Ⅲ, was identified by UV spectra, MS, and the 1H-NMR and 13C-NMR spectra. Results: Good linearity was obtained within the range of 5 and 2000 ng/mL (r>0.99 using a linear regression model with 1/x2 weighting) for acetylshikonin. The interday and intraday precisions were found to be less than 12.3%, with the exception of the lowest concentration, which was less than 17.2%. The interday and intraday accuracies, which were between -3% and 0.6%, were also observed. After the administration of acetylshikonin (80 mg/kg, po) in macaque monkeys, the pharmacokinetic parameters were obtained through the non-compartmental analysis, where the area under the concentration-time curve to the last measurable concentration, the terminal elimination half-10.2±0.7 h, respectively. Conclusion: The method was validated and applied to the quantitative determination and pharmacokinetic study of aeetylshikonin in the blood samples of macaque monkeys.

  18. Development of a disposable magnetically levitated centrifugal blood pump (MedTech Dispo) intended for bridge-to-bridge applications--two-week in vivo evaluation.

    Science.gov (United States)

    Nagaoka, Eiki; Someya, Takeshi; Kitao, Takashi; Kimura, Taro; Ushiyama, Tomohiro; Hijikata, Wataru; Shinshi, Tadahiko; Arai, Hirokuni; Takatani, Setsuo

    2010-09-01

    Last year, we reported in vitro pump performance, low hemolytic characteristics, and initial in vivo evaluation of a disposable, magnetically levitated centrifugal blood pump, MedTech Dispo. As the first phase of the two-stage in vivo studies, in this study we have carried out a 2-week in vivo evaluation in calves. Male Holstein calves with body weight of 62.4–92.2 kg were used. Under general anesthesia, a left heart bypass with a MedTech Dispo pump was instituted between the left atrium and the descending aorta via left thoracotomy. Blood-contacting surface of the pump was coated with a 2-methacryloyloxyethyl phosphorylcholine polymer. Post-operatively, with activated clotting time controlled at 180–220 s using heparin and bypass flow rate maintained at 50 mL/kg/min, plasma-free hemoglobin (Hb), coagulation, and major organ functions were analyzed for evaluation of biocompatibility. The animals were electively sacrificed at the completion of the 2-week study to evaluate presence of thrombus inside the pump,together with an examination of major organs. To date, we have done 13 MedTech Dispo implantations, of which three went successfully for a 2-week duration. In these three cases, the pump produced a fairly constant flow of 50 mL/Kg/min. Neurological disorders and any symptoms of thromboembolism were not seen. Levels of plasma-free Hb were maintained very low. Major organ functions remained within normal ranges. Autopsy results revealed no thrombus formation inside the pump. In the last six cases, calves suffered from severe pneumonia and they were excluded from the analysis. The MedTech Dispo pump demonstrated sufficient pump performance and biocompatibility to meet requirements for 1-week circulatory support. The second phase (2-month in vivo study) is under way to prove the safety and efficacy of MedTech Dispo for 1-month applications.

  19. 32 CFR 552.77 - Suspension approval.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Suspension approval. 552.77 Section 552.77 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY MILITARY RESERVATIONS AND....77 Suspension approval. The installation commander will personally approve all cases in...

  20. 40 CFR 123.61 - Approval process.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 21 2010-07-01 2010-07-01 false Approval process. 123.61 Section 123... REQUIREMENTS Program Approval, Revision, and Withdrawal § 123.61 Approval process. (a) After determining that a...; and (6) Briefly outline the fundamental aspects of the State's proposed program, and the process...

  1. 40 CFR 145.31 - Approval process.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Approval process. 145.31 Section 145.31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) STATE UIC PROGRAM REQUIREMENTS Program Approval, Revision and Withdrawal § 145.31 Approval process....

  2. 12 CFR 611.510 - Approval procedures.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Approval procedures. 611.510 Section 611.510 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM ORGANIZATION Transfer of Authorities § 611.510 Approval procedures. (a) Upon receipt of approval of a resolution by the Farm...

  3. Quantitative spatially resolved measurement of tissue chromophore concentrations using photoacoustic spectroscopy: application to the measurement of blood oxygenation and haemoglobin concentration

    Energy Technology Data Exchange (ETDEWEB)

    Laufer, Jan; Delpy, Dave; Elwell, Clare; Beard, Paul [Department of Medical Physics and Bioengineering, University College London, Malet Place Engineering Building, London WC1E 6BT (United Kingdom)

    2007-01-07

    A new approach based on pulsed photoacoustic spectroscopy for non-invasively quantifying tissue chromophore concentrations with high spatial resolution has been developed. The technique is applicable to the quantification of tissue chromophores such as oxyhaemoglobin (HbO{sub 2}) and deoxyhaemoglobin (HHb) for the measurement of physiological parameters such as blood oxygen saturation (SO{sub 2}) and total haemoglobin concentration. It can also be used to quantify the local accumulation of targeted contrast agents used in photoacoustic molecular imaging. The technique employs a model-based inversion scheme to recover the chromophore concentrations from photoacoustic measurements. This comprises a numerical forward model of the detected time-dependent photoacoustic signal that incorporates a multiwavelength diffusion-based finite element light propagation model to describe the light transport and a time-domain acoustic model to describe the generation, propagation and detection of the photoacoustic wave. The forward model is then inverted by iteratively fitting it to measurements of photoacoustic signals acquired at different wavelengths to recover the chromophore concentrations. To validate this approach, photoacoustic signals were generated in a tissue phantom using nanosecond laser pulses between 740 nm and 1040 nm. The tissue phantom comprised a suspension of intralipid, blood and a near-infrared dye in which three tubes were immersed. Blood at physiological haemoglobin concentrations and oxygen saturation levels ranging from 2% to 100% was circulated through the tubes. The signal amplitude from different temporal sections of the detected photoacoustic waveforms was plotted as a function of wavelength and the forward model fitted to these data to recover the concentrations of HbO{sub 2} and HHb, total haemoglobin concentration and SO{sub 2}. The performance was found to compare favourably to that of a laboratory CO-oximeter with measurement resolutions of {+-}3

  4. The research and development and application of computer system in safe blood transfusion%计算机系统在安全输血中的研发与应用

    Institute of Scientific and Technical Information of China (English)

    邢月利; 王艳春; 高乐俊; 刘静

    2015-01-01

    With the constant progress of blood transfusion, the application of the computer system has become the mainstream in the development of blood transfusion. In order to ensure the security of blood transfusion and improve work efficiency, we researched and used the blood transfusion management system in our hospital from January 2010. This system played a huge advantage and role in our hosptial's review of "first-class hospital" in 2013 , made the safety of blood transfusion and reduced the work intensity of the workers. This system includes a transfusion application, nursing practice, cross matching,blood inventory management, send blood and other functions. At the same time, this sysyem connects with hospital information. This system provides a set of accurate, convenient management tool through the application of the software for transfusion branch.%随着输血事业的不断进步,计算机系统的应用成为输血发展的主流.为保障输血安全,提高工作效率,我们于2010 年1月正式研发并运用了输血管理系统,在2013年我院的"三甲医院"复审的过程中发挥了巨大的优势和作用,保证了输血的安全性并减轻了工作人员的工作强度.此系统包括输血申请、护理采血 、配血、 血液库存、发血等功能.同时与医院信息系统共联 , 通过这套软件的应用为输血科提供了一套准确无误、方便快捷的管理工具.

  5. Managing your blood sugar

    Science.gov (United States)

    Hyperglycemia - control; Hypoglycemia - control; Diabetes - blood sugar control; Blood glucose - managing ... Know how to: Recognize and treat low blood sugar (hypoglycemia) Recognize and treat high blood sugar (hyperglycemia) ...

  6. Hunan Rare Earth Group Approved

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    <正>Following Guangdong,Guangxi,Fujian and Jiangxi,Hunan announced that it would consolidate its rare earth resources-the consolidation plan of Hunan Rare Earth Group has been approved. Consolidation of the rare earth industry of south China is in full swing.According to "Several Opinions of the State Council on Promoting the Sustainable and Healthy Development of Rare Earth Industry"(hereinafter referred to as "Several Opinions")released in 2011,

  7. Nelfinavir: fourth protease inhibitor approved.

    Science.gov (United States)

    1997-01-01

    The Food and Drug Administration (FDA) has granted accelerated approval to nelfinavir in both adult and pediatric formulations. Agouron, the manufacturer, used innovative computerized drug design techniques to discover, design, and refine the nelfinavir molecule. Nelfinavir is marketed under the trade name Viracept, and costs $5,000 per year. Early clinical trials find it to be as powerful as the other protease inhibitors, but with a different resistance profile. The drug has relatively few drug indications; however, several compounds have been contraindicated.

  8. 人造血管在维持血液透析患者中的应用和护理方法%Application and nursing method of artificial blood vessel in maintenance hemodialysis patients

    Institute of Scientific and Technical Information of China (English)

    杨波; 刘晓玲

    2016-01-01

    Objective:To explore the application and nursing methods of artificial blood vessel in maintenance hemodialysis patients.Methods:2 cases of hemodialysis patients who were treated with artificial blood vessels were selected.We observed the internal fistula situation of the artificial blood vessels,and to explore the corresponding nursing methods.Results:2 cases of internal fistula in artificial blood vessels were unobstructed;the blood flow remained at 200~280 mL/min;there were no postoperative complications occurred.Conclusion:The application of artificial blood vessels in maintenance hemodialysis patients has significant effect.It can protect the normal circulation of blood effectively,and it has less complications.%目的:探讨人造血管在维持血液透析患者中的应用和护理方法。方法:收治使用人造血管进行透析患者2例,观察患者人造血管的内瘘情况,并探讨相应的护理方法。结果:2例患者的人造血管内瘘全部通畅,血流量保持在200~280 mL/min,术后无并发症发生。结论:人造血管在维持血液透析患者中的应用效果显著,能有效保障血液的正常流转,并发症少。

  9. 探讨ABO血型梯形微型板在数字血型仪中的应用%Application of ABO blood group of trapezoid micro plate in adigital type instrument

    Institute of Scientific and Technical Information of China (English)

    刘涛; 桑仁贵; 李茂恩

    2014-01-01

    Objective:The evaluation trapezoid micro-plate method to detect ABO blood group.Method:use auto-matic sampling system and Blood Group's apparetus to analyse specimen's blood group.Result:The detection of the blood group of 9619 blood donors anti-coagulation specimens, were 99.7% correct.Conclusion:The trapezoid mi-cro-plate method is simple, accurate, high sensitivity,routine detection of blood type is applicable to bulk sample.%目的:评价梯形微型板在血型检测中的应用。方法:全自动加样系统加样,血型判读仪进行判读。结果:通过对9619例无偿献血者血液抗凝标本的血型检测,正确率99.7%。结论:梯形微型板方法操作简便,结果准确,灵敏度高,适用于大批量样本的血型常规检测。

  10. Blood Basics

    Science.gov (United States)

    ... of ASH ASH Meeting on Hematologic Malignancies Consultative Hematology Course ASH Meeting on Lymphoma Biology ASH Workshop on Genome Editing Publications Blood The Hematologist ASH Clinical News ASH Self-Assessment Program Hematology , ASH Education Program About Awards Membership ASH Foundation ...

  11. Blood Clots

    Science.gov (United States)

    ... of ASH ASH Meeting on Hematologic Malignancies Consultative Hematology Course ASH Meeting on Lymphoma Biology ASH Workshop on Genome Editing Publications Blood The Hematologist ASH Clinical News ASH Self-Assessment Program Hematology , ASH Education Program About Awards Membership ASH Foundation ...

  12. Enantioselective analysis of citalopram and demethylcitalopram in human whole blood by chiral LC-MS/MS and application in forensic cases

    DEFF Research Database (Denmark)

    Johansen, Sys Stybe

    2017-01-01

    , a chiral liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is developed for the measurement of citalopram and demethylcitalopram enantiomers in whole blood and is applied to forensic cases. Whole blood samples (0.10 g) were extracted with butyl acetate after adjusting the pH with 2 M Na...... to authentic blood samples from forensic investigations demonstrating that escitalopram was less frequent than citalopram among all forensic cases....

  13. Morphology of drying blood pools

    Science.gov (United States)

    Laan, Nick; Smith, Fiona; Nicloux, Celine; Brutin, David; D-Blood project Collaboration

    2016-11-01

    Often blood pools are found on crime scenes providing information concerning the events and sequence of events that took place on the scene. However, there is a lack of knowledge concerning the drying dynamics of blood pools. This study focuses on the drying process of blood pools to determine what relevant information can be obtained for the forensic application. We recorded the drying process of blood pools with a camera and measured the weight. We found that the drying process can be separated into five different: coagulation, gelation, rim desiccation, centre desiccation, and final desiccation. Moreover, we found that the weight of the blood pool diminishes similarly and in a reproducible way for blood pools created in various conditions. In addition, we verify that the size of the blood pools is directly related to its volume and the wettability of the surface. Our study clearly shows that blood pools dry in a reproducible fashion. This preliminary work highlights the difficult task that represents blood pool analysis in forensic investigations, and how internal and external parameters influence its dynamics. We conclude that understanding the drying process dynamics would be advancement in timeline reconstitution of events. ANR funded project: D-Blood Project.

  14. Clinical application of Sleeping Beauty and artificial antigen presenting cells to genetically modify T cells from peripheral and umbilical cord blood.

    Science.gov (United States)

    Huls, M Helen; Figliola, Matthew J; Dawson, Margaret J; Olivares, Simon; Kebriaei, Partow; Shpall, Elizabeth J; Champlin, Richard E; Singh, Harjeet; Cooper, Laurence J N

    2013-02-01

    The potency of clinical-grade T cells can be improved by combining gene therapy with immunotherapy to engineer a biologic product with the potential for superior (i) recognition of tumor-associated antigens (TAAs), (ii) persistence after infusion, (iii) potential for migration to tumor sites, and (iv) ability to recycle effector functions within the tumor microenvironment. Most approaches to genetic manipulation of T cells engineered for human application have used retrovirus and lentivirus for the stable expression of CAR(1-3). This approach, although compliant with current good manufacturing practice (GMP), can be expensive as it relies on the manufacture and release of clinical-grade recombinant virus from a limited number of production facilities. The electro-transfer of nonviral plasmids is an appealing alternative to transduction since DNA species can be produced to clinical grade at approximately 1/10(th) the cost of recombinant GMP-grade virus. To improve the efficiency of integration we adapted Sleeping Beauty (SB) transposon and transposase for human application(4-8). Our SB system uses two DNA plasmids that consist of a transposon coding for a gene of interest (e.g. 2(nd) generation CD19-specific CAR transgene, designated CD19RCD28) and a transposase (e.g. SB11) which inserts the transgene into TA dinucleotide repeats(9-11). To generate clinically-sufficient numbers of genetically modified T cells we use K562-derived artificial antigen presenting cells (aAPC) (clone #4) modified to express a TAA (e.g. CD19) as well as the T cell costimulatory molecules CD86, CD137L, a membrane-bound version of interleukin (IL)-15 (peptide fused to modified IgG4 Fc region) and CD64 (Fc-γ receptor 1) for the loading of monoclonal antibodies (mAb)(12). In this report, we demonstrate the procedures that can be undertaken in compliance with cGMP to generate CD19-specific CAR(+) T cells suitable for human application. This was achieved by the synchronous electro-transfer of

  15. Reduction of low-density lipoprotein cholesterol, plasma viscosity, and whole blood viscosity by the application of pulsed corona discharges and filtration.

    Science.gov (United States)

    Jung, Jin M; Fridman, Alexander; Cho, Daniel J; Cho, Young I

    2013-03-01

    The present study investigated the feasibility of applying pulsed corona discharges to blood plasma to reduce the viscosity of blood plasma and whole blood. Blood plasma was separated from blood cells, treated with corona discharges, and filtered before it was re-mixed with blood cells. Plasma viscosity (PV), whole blood viscosity (WBV), and low-density lipoprotein (LDL)-c concentration were measured before and after the corona treatment and filtration. Both PV and WBV increased in the case of the corona treatment only, whereas both of them decreased in the case of the corona treatment plus filtration. In particular, the LDL-c decreased in the case of the corona treatment plus filtration by 31.5% from the baseline value. The effect of the corona treatment on the reduction of the WBV was significant at low shear rates, but not at high shear rates, suggesting that the precipitation of the molecules in blood plasma by the corona treatment and subsequent removal may suppress the aggregation of erythrocytes and improve rheological properties of blood.

  16. Blood pressure measurement

    Science.gov (United States)

    Diastolic blood pressure; Systolic blood pressure; Blood pressure reading; Measuring blood pressure ... or your health care provider will wrap the blood pressure cuff snugly around your upper arm. The lower ...

  17. Blood Count Tests

    Science.gov (United States)

    Your blood contains red blood cells (RBC), white blood cells (WBC), and platelets. Blood count tests measure the number and types of cells in your blood. This helps doctors check on your overall health. ...

  18. French approval procedures for pyrotechnical automotive safety equipments

    OpenAIRE

    Aufauvre, Lionel; Branka, Ruddy

    2005-01-01

    International audience; Pyrotechnical articles for civil uses may be subject to national procedures before placing on the market According to the French decree n°90-153, 16 February 1990 as modified; explosives that are dispensed with EC marking and that are not excluded of the decree application have to conform to approved types. Pyrotechnical automotive safety equipments such gas generators for airbag modules or seat-belt pretensioners, pyrotechnie relay compositions and/or igniters inside ...

  19. 回收式自体输血在脊柱外科手术中的应用体会%Recycling type application experience of autologous blood transfusion in spinal surgery

    Institute of Scientific and Technical Information of China (English)

    樊晓建; 杨召; 张超; 李宏键; 胡建华

    2015-01-01

    目的:分析探讨回收式自体输血技术在脊柱外科手术中应用的体会。方法:选择术中可能出血较多脊柱外科常规手术的30例患者术中使用自体血回输仪。术后将处理后的浓缩红细胞回输给患者。结果:本组所有病例顺利完成了自体血液回收,生命体征平稳,无输血反应,无感染等并发症。平均每例患者自体血液回输(240±160)ml,共回收自体血液5590ml。结论:术中回收式自体血液方便安全,既解决了血源短缺问题,又可避免输入异体血而引起的输血反应及引发感染病的可能性,是安全有效的血液回输技术。%Objective Retrospective analysis to investigate the recovery of autologous blood transfusion technology application experience in spinal surgery.Methods Choose intraoperative bleeding may be more significant spine routine operation of 30 cases of patients who use of autologous blood doping. Postoperative will process after the enrichment of red blood cells back to patients.Results This group of all cases successfully completed the autologous blood recovery, stable vital signs, no blood transfusion reaction, no complications such as infection. , on average, each patient blood doping (240+ 160) of autologous ml, recycling of autologous blood 5590 ml.Conclusions Intraoperative autologous blood recovery type convenient and safe, both can solve the problem of blood shortages, but also avoid the input variants caused by blood transfusion reactions and the possibility of infection disease, blood doping technology is safe and effective.

  20. Bridging the gap between sample collection and laboratory analysis: using dried blood spots to identify human exposure to chemical agents

    Science.gov (United States)

    Hamelin, Elizabeth I.; Blake, Thomas A.; Perez, Jonas W.; Crow, Brian S.; Shaner, Rebecca L.; Coleman, Rebecca M.; Johnson, Rudolph C.

    2016-05-01

    Public health response to large scale chemical emergencies presents logistical challenges for sample collection, transport, and analysis. Diagnostic methods used to identify and determine exposure to chemical warfare agents, toxins, and poisons traditionally involve blood collection by phlebotomists, cold transport of biomedical samples, and costly sample preparation techniques. Use of dried blood spots, which consist of dried blood on an FDA-approved substrate, can increase analyte stability, decrease infection hazard for those handling samples, greatly reduce the cost of shipping/storing samples by removing the need for refrigeration and cold chain transportation, and be self-prepared by potentially exposed individuals using a simple finger prick and blood spot compatible paper. Our laboratory has developed clinical assays to detect human exposures to nerve agents through the analysis of specific protein adducts and metabolites, for which a simple extraction from a dried blood spot is sufficient for removing matrix interferents and attaining sensitivities on par with traditional sampling methods. The use of dried blood spots can bridge the gap between the laboratory and the field allowing for large scale sample collection with minimal impact on hospital resources while maintaining sensitivity, specificity, traceability, and quality requirements for both clinical and forensic applications.

  1. Analysis the construction situation of cord blood bank and application of umbilical cord blood collection in neonatal related diseases at present%脐血库建设现状及脐带血采集在新生儿相关疾病中的应用

    Institute of Scientific and Technical Information of China (English)

    刘红; 南虎松

    2015-01-01

    Umbilical cord blood refers to the blood that remains in the placenta and umbilical cord after fetus umbilical cord ligation and transection.In recent years,scientific research found that umbilical cord blood has special value,as it contains hematopoietic stem cells,which has become an important source of hematopoietic stem cells.At present,umbilical cord blood has been used in the clinical more and more widely,in this study,we want to summarized construction situation of cord blood bank,and its application in the diagnosis and treatment on neonatal related diseases.%脐带血是指胎儿娩出、脐带结扎并离断后残留在胎盘和脐带中的血液。近年的科学研究发现,脐带血的特殊价值,在于它含有造血干细胞,已成为造血干细胞的重要来源。目前,脐带血在临床中的应用越来越广泛,现对我国脐血库建设现状及脐带血采集在新生儿相关疾病诊断、治疗方面的应用做一综述。

  2. Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET : Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [C-11]MPDX

    NARCIS (Netherlands)

    Sijbesma, Jürgen W A; Zhou, Xiaoyun; Vállez García, David; Houwertjes, Martin C; Doorduin, Janine; Kwizera, Chantal; Maas, Bram; Meerlo, Peter; Dierckx, Rudi A; Slart, Riemer H J A; Elsinga, Philip H; van Waarde, Aren

    2016-01-01

    Small animal positron emission tomography (PET) can be used to detect small changes in neuroreceptor availability. This often requires rapid arterial blood sampling. However, current catheterization procedures do not allow repeated blood sampling. We have developed a procedure which allows arterial

  3. Effect of catecholamine-receptor stimulating agents on blood pressure after local application in the nucleus tractus solitarii of the medulla oblongata

    NARCIS (Netherlands)

    Zandberg, P.; Jong, Wybren de; Wied, D. de

    1979-01-01

    The effect of various catecholamines and α-mimetics, given by microinjection in the A2-region of the nucleus tractus solitarii (NTS), on blood pressure was investigated in anesthetizedmale rats. A dose-dependent decrease of blood pressure and heart rate was induced by adrenaline as the most effectiv

  4. Effects of blood-feeding on olfactory sensitivity of the malaria mosquito Anopheles gambiae: application of mixed linear models to account for repeated measurements

    NARCIS (Netherlands)

    Qiu, Y.T.; Gort, G.; Torricelli, A.; Takken, W.; Loon, van J.J.A.

    2013-01-01

    Olfaction plays an important role in the host-seeking behavior of the malaria mosquito Anopheles gambiae. After a complete blood meal, female mosquitoes will not engage in host-seeking behavior until oviposition has occurred. We investigated if peripheral olfactory sensitivity changed after a blood

  5. 抗体筛查细胞抗原涵盖范围与电子配血技术的安全应用%Application of antibody screening cell antigen coverage and electronic matching of blood

    Institute of Scientific and Technical Information of China (English)

    刘家瑞; 王远杰; 冯宁; 李红梅; 冯俊程

    2013-01-01

    Objective To study the significance of antibody screening cell antigen range in the electronic matching technology security application.Methods ABO/RhD of blood donor and blood recipient were detect with blood type and antibody.The blood matching were used by electronic matching technology and polybrene crossmatch.Results Of 22,790 blood samples of patients,there were 164 cases with positive antibody screening (0.72%).Of 15740 blood donor blood samples,there were 11 cases with positive antibody screening (0.07 %).Of 22,540 blood samples in line with the rules of the electronic distribution of blood,the implementation of electronic matching of blood ABO / RhD blood group incompatibility was not found by the computer.Of 22534 blood samples with blood compatible,6 blood samples polybrene crossmatch was incompatible with the main side and found no secondary side incompatible.6 blood samples spectrum cell antibody specific identification of all the MNS system anti-Mur,missed rate of 0.027%.Conclusion Clinical antibody screening cells only serological crossmatch,whose antibody missing rate is relatively high,which cannot fully protect the security of the technology of the electronic matching of blood.%目的 探讨抗体筛查细胞抗原涵盖范围在保障电子配血技术安全应用中的意义.方法 分别对供受血者进行ABO/RhD血型检测以及抗体筛查.患者二次血型鉴定结果一致,抗体筛查结果阴性,按电子配血规则进行电子配血,同时采用凝聚胺法对供受血者进行传统血清学交叉配血平行试验,分析抗体筛查细胞抗原涵盖范围与不规则抗体漏检情况以及对电子配血技术安全应用的影响.结果 22790份患者血样,抗体筛查阳性164例,阳性率0.72%.15740份供血者血样,抗体筛查阳性11例,占0.07%.22540份血样均符合电子配血规则,由计算机实施电子配血未发现ABO/RhD血型不相容.用凝聚胺法进行传统血清学

  6. Repurposing of approved cardiovascular drugs.

    Science.gov (United States)

    Ishida, Junichi; Konishi, Masaaki; Ebner, Nicole; Springer, Jochen

    2016-09-20

    Research and development of new drugs requires both long time and high costs, whereas safety and tolerability profiles make the success rate of approval very low. Drug repurposing, applying known drugs and compounds to new indications, has been noted recently as a cost-effective and time-unconsuming way in developing new drugs, because they have already been proven safe in humans. In this review, we discuss drug repurposing of approved cardiovascular drugs, such as aspirin, beta-blockers, angiotensin converting enzyme inhibitors, angiotensin II receptor blockers, cardiac glycosides and statins. Regarding anti-tumor activities of these agents, a number of experimental studies have demonstrated promising pleiotropic properties, whereas all clinical trials have not shown expected results. In pathological conditions other than cancer, repurposing of cardiovascular drugs is also expanding. Numerous experimental studies have reported possibilities of drug repurposing in this field and some of them have been tried for new indications ('bench to bedside'), while unexpected results of clinical studies have given hints for drug repurposing and some unknown mechanisms of action have been demonstrated by experimental studies ('bedside to bench'). The future perspective of experimental and clinical studies using cardiovascular drugs are also discussed.

  7. Application of a Liquid Extraction Based Sealing Surface Sampling Probe for Mass Spectrometric Analysis of Dried Blood Spots and Mouse Whole-Body Thin Tissue Sections

    Energy Technology Data Exchange (ETDEWEB)

    Van Berkel, Gary J [ORNL; Kertesz, Vilmos [ORNL

    2009-01-01

    The utility of a liquid extraction based sealing surface sampling probe (SSSP) for the direct mass spectrometric analysis of targeted drugs and metabolites in dried blood spots (DBSs) and whole mouse thin tissue sections was demonstrated. The accuracy and precision for the quantitative analysis of a minimum of 50 ng/mL sitamaquine or acetaminophen in DBSs on paper were well within the required 15% dictated by internationally recognized acceptance criteria for assay validations. Analysis of whole-body mouse thin tissue sections from animals dosed with propranolol, adhered to an adhesive tape substrate, provided semi-quantitative information for propranolol and its hydroxyproranolol glucuronide metabolite within specific organs of the tissue. The relative abundances recorded for the two compounds in the brain, lung, kidney and liver were in nominal agreement with previously reported amounts based on analysis using a liquid microjunction surface sampling probe (LMJ-SSP), and whole-body autoradiography (WBA) and HPLC-MS analysis. The ability to sample and analyze from tape-adhered tissue samples, which are generally employed in WBA analysis, presents the possibility of consecutive WBA and SSSP-MS analysis of the same tissue section. This would facilitate assignment, and possibly quantitation, of the different molecular forms of total drug related material detected in the WBA analysis. The flexibility to sample larger or smaller spot sizes, alternative probe sealing mechanisms, and a reduction in internal volumes and associated sample carryover issues will be among the first simple improvements necessary to make the SSSP-MS method a practical DBS and/or thin tissue section analysis tool or to expand its use to other surface sampling applications.

  8. Red-blood-cell-like BSA/Zn3(PO4)2 hybrid particles: Preparation and application to adsorption of heavy metal ions

    Science.gov (United States)

    Zhang, Baoliang; Li, Peitao; Zhang, Hepeng; Li, Xiangjie; Tian, Lei; Wang, Hai; Chen, Xin; Ali, Nisar; Ali, Zafar; Zhang, Qiuyu

    2016-03-01

    A novel kind of red-blood-cell-like bovine serum albumin (BSA)/Zn3(PO4)2 hybrid particle is prepared at room temperature by a facile and rapid one-step method based on coordination between BSA and zinc ion. The morphology of the monodisperse hybrid particle shows oblate spheroidal type with a one sided single hole on the surface. The hybrid particle is constructed with BSA/Zn3(PO4)2 nanoplates of 35 nm thick. The average particle size of hybrid particle is 2.3 μm, and its BET specific surface area is 146.64 cm2/g. To clarify the evolution of BSA/Zn3(PO4)2 hybrid particle, SEM and elemental analysis as a function of particle growth time are investigated. The formation mechanism of BSA/Zn3(PO4)2 hybrid particle, which can be described as crystallization, coordination and self-assembly process, is illustrated in detail. The as-prepared BSA/Zn3(PO4)2 hybrid particle is used for adsorption of Cu2+. The hybrid particle displayed excellent adsorption properties on Cu2+. The adsorption efficiency of BSA/Zn3(PO4)2 hybrid particles at 5 min and 30 min are 86.33% and 98.9%, respectively. The maximum adsorption capacity is 6.85 mg/g. Thus, this kind of novel adsorbent shows potential application value in ultra-fast and highly efficient removal of Cu2+.

  9. OMB Recommended vs Approved Operating Budget

    Data.gov (United States)

    Montgomery County of Maryland — This dataset includes the Fiscal Year 2015 County Executive Recommended and County Council Approved operating budgets for Montgomery County, for comparison purposes....

  10. Application of invasive blood pressure monitoring in critically ill neonates and the nursing points%有创血压监测在危重新生儿的应用及护理

    Institute of Scientific and Technical Information of China (English)

    张忠菊; 石小娟; 陈丽莲

    2013-01-01

    Objective To investigate the application of invasive blood pressure monitoring in critically ill neonates and the main points Of nursing. Methods Thirty critically ill neonates in the Neonatal Intensive Care Unit (NICU) were performed invasive arterial blood pressure monitoring continually. Results Timely treatment was given to all the patients according to the dynamic disease situation provided by invasive blood pressure monitoring, which reduced the adverse stimulations caused by pain to the neonates, and greatly alleviated the nurse's workload. No complications occurred during the whole process. Conclusion The arterial blood pressure monitoring provides reliable data of arterial blood pressure, which plays a very important role in the treatment of patients in NICU.%目的 通过对有创血压(IBP)监测的护理,探讨有创血压监测在新生儿重症患者中的作用及其护理要点.方法 对30例NICU新生儿重症患儿进行持续动脉血压监测.结果 30例患儿根据有创血压提供的病情动态变化,及时处理,并未出现并发症.结论 有创血压监测提供了可靠的持续动态血压数据,在NICU患者救治中起到积极重要的作用.

  11. 血涂片细胞形态检查在初诊患者临床诊断中的应用%Blood smear the application of the cell morphology was in clinical diagnosis

    Institute of Scientific and Technical Information of China (English)

    滕瑞军

    2015-01-01

    Objective To study the blood smear cells form to check the application result was in clinical diagnosis. Meth-ods Selecting in June 2013 to May 2014, our hospital 380 cases of patients with both the fever, adopt blood smear cell morpho-logical examination, observation of red blood cells, white blood cells, platelets, smear tail abnormal morphology and abnormal cells. Results 380 patients in the final diagnosis of 136 cases of bacterial infection, blood routine examination is normal, the blood smears neutrophil particles in plasma and enlargement, and poisoning phenomenon; 45 cases of iron-deficiency anaemia, the red blood cells form is differ, the cell body is small, light colored areas significantly increased; Giant cell anemia in 59, ma-ture red blood cells form is differ, the main oval, big cell body; Myelodysplastic syndrome 32 cases, red blood cell sizes, see more huge platelets and polymorphic, abnormal red blood cells; 45 cases of acute leukemia, immature cells, red blood cells. conclusion The blood smear cell morphological examination to observe the platelets, white blood cells and red blood cell mor-phology, preliminarily determine whether patients are blood disease, bacterial infection, anemia, such as simple operation, easy to grasp, was to the patient's clinical diagnosis is of high value.%目的:探讨血涂片细胞形态检查在初诊患者临床诊断中的应用效果。方法选取2013年6月至2014年5月我院收治的初诊发热患者380例,均采用血涂片细胞形态检查,观察红细胞、白细胞、血小板形态、涂片尾部细胞异常形态和异常物。结果380例患者中最终确诊细菌感染136例,血常规检查正常,血涂片检查中性粒细胞浆内颗粒增粗、增多和中毒现象;缺铁性贫血45例,红细胞形态不一,胞体较小,淡染区明显扩大;巨幼细胞性贫血59例,成熟红细胞形态不一,主要呈椭圆形,胞体偏大;骨髓增生异常综合征32例,红

  12. US Army blood program: 2025 and beyond.

    Science.gov (United States)

    Gonzales, Richard; Taylor, Audra L; Atkinson, Andrew J; Malloy, Wilbur W; Macdonald, Victor W; Cap, Andrew P

    2016-03-01

    In preparing to support the Army in 2025 and beyond, the Army Blood Program remains actively engaged with the research and advanced development of blood products and medical technology to improve blood safety and efficacy in conjunction with the US Army Medical Research and Materiel Command. National and International Blood Bank authorities have noted that the US Army research and development efforts in providing new blood products and improving blood safety operate on the cutting edge of technology and are transformational for the global blood industry. Over the past 14 years, the Army has transformed how blood support is provided and improved the survival rate of casualties. Almost every product or process developed by or for the military has found an application in treating civilian patients. Conflicts have many unwanted consequences; however, in times of conflict, one positive aspect is the identification of novel solutions to improve the safety and efficacy of the blood supply.

  13. Low Blood Glucose (Hypoglycemia)

    Science.gov (United States)

    ... Disease, & Other Dental Problems Diabetes & Sexual & Urologic Problems Low Blood Glucose (Hypoglycemia) What is hypoglycemia? Hypoglycemia, also called low blood glucose or low blood sugar, occurs when ...

  14. Investigation on artificial blood or substitute blood replace the natural blood.

    Science.gov (United States)

    Keyhanian, Sh; Ebrahimifard, M; Zandi, M

    2014-01-01

    Blood is a liquid tissue in which dissolved with abundant chemical factors and millions of different cells The reduction of unwanted side effects, especially diseases that emerge through blood such as HIV and hepatitis, has a significant role for modern medicine of transfusion and transplantation. The issues and costs of human blood collection and storage, direct this procedure towards the use of alternatives blood. Two important research fields of this area were oxygen carriers based on hemoglobin and perfluoro chemicals. While they do not have the same quality as the blood cell products, the oxygen carrier solutions have potential clinical and non-clinical applications. The result showed that these products can reach to the body tissues easier than normal red blood cells, and can control the oxygen directly. The final aim of transfusion is to establish a transfusion system with no side effects, and the fact that oxygen carrier artificial blood has this property. The article attempts to step towards solving some problems of blood transfusion through describing the properties of artificial blood alternatives.

  15. Evaluation on clinical application of XE-2100 blood cell automatic analyzer for nucleated red blood cell count%XE-2100全自动血细胞分析仪对有核红细胞分析的应用评价

    Institute of Scientific and Technical Information of China (English)

    杨晓枫

    2013-01-01

    Objective To evaluate the veracity of nucleated red blood cell ( NRBC ) count by Sysmex XE - 2100 automatic blood cell analyzer. Methods The peripheral blood NRBC counts of 139 patients were examined by both Sysmex XE - 2100 automatic blood cell analyzer and microscopy. Results The counted results of high value and medium value had no significant difference between manual and automatic methods ( P 〉 0.05 ), the counted results of low value had significant difference between manual and automatic methods ( P 〈 0.05 ). The method by automatic analyzer had fine reproducibility. Conclusion The application of XE - 2100 automatic blood cell analyzer for NRBC count is quick and accurate , and it can provide accurate blood cell monitoring data for clinicians.%目的 评价Sysmex XE-2100全自动血细胞分析仪对有核红细胞(NRBC)分析结果的准确性.方法 采用仪器法和显微镜计数法分别对2006年1月至2011年2月间139例外周血NRBC阳性的临床血液样本进行测定.结果 仪器法对NRBC的检测结果与显微镜计数法比较,中、高值差异无统计学意义(P>0.05),低值差异有统计学意义(P<0.05).仪器的重复性良好.结论 Sysmex XE-2100全自动血细胞分析仪分析结果准确、快速,可为临床医生提供准确血细胞监测数据.

  16. Isolating cells from female/male blood mixtures using florescence in situ hybridization combined with low volume PCR and its application in forensic science.

    Science.gov (United States)

    Feng, Lei; Li, Cai-Xia; Han, Jun-Ping; Xu, Cheng; Hu, Lan

    2015-11-01

    To obtain single-source short tandem repeat (STR) profiles in trace female/male blood mixture samples, we combined florescence in situ hybridization (FISH), laser microdissection, and low volume PCR (LV-PCR) to isolate male/female cells and improve sensitivity. The results showed that isolation of as few as 10 leukocytes was sufficient to yield full STR profiles in fresh female or male blood samples for 32 independent tests with a low additional alleles rate (3.91%) and drop-out alleles rate (5.01%). Moreover, this procedure was tested in two fresh blood mixture series at three ratios (1:5, 1:10, and 1:20), two mock female/male blood mixture casework samples, and one practical casework sample. Male and female STR profiles were successfully detected in all of these samples, showing that this procedure could be used in forensic casework in the future.

  17. 14 CFR 21.325 - Export airworthiness approvals.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Export airworthiness approvals. 21.325... AIRCRAFT CERTIFICATION PROCEDURES FOR PRODUCTS AND PARTS Export Airworthiness Approvals § 21.325 Export airworthiness approvals. (a) Kinds of approvals. (1) Export airworthiness approval of Class I products is...

  18. Double-echo perfusion-weighted MR imaging: basic concepts and application in brain tumors for the assessment of tumor blood volume and vascular permeability

    OpenAIRE

    Uematsu, Hidemasa; Maeda, Masayuki

    2006-01-01

    Perfusion-weighted magnetic resonance (MR) imaging using contrast agents plays a key role in characterizing tumors of the brain. We have shown that double-echo perfusion-weighted MR imaging (DEPWI) is potentially useful in assessing brain tumors. Quantitative indices, such as tumor blood volume, are obtained using DEPWI, which allows correction of underestimation of tumor blood volume due to leakage of contrast agents from tumor vessels, in addition to simultaneous acquisition of tumor vessel...

  19. Application of nucleic acid detection technology in blood screening of blood donors%核酸检测技术在献血人员血液筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    柯苑; 赵静; 马成平; 黄敏; 姚慧兰; 张立波; 马贵明

    2014-01-01

    Objective To explore the value of nucleic acid detection technology (NAT ) for blood screening to decrease the infection induced by blood donors in the window period and infected insidiously .Methods A total of 54 358 blood samples were detected by ELISA for hepatitis B surface antigen (HBsAg) ,antibody of human immuno-deficiency virus (HIV-Ab ) ,antibody of hepatitis C virus (anti-HCV ) ,and transcription-mediated amplification (TMA)method were used to detecte the DNA of hepatitis B virus (HBV-DNA ) ,the RNA of hepatitis C virus (HCV-RNA)and the RNA of type 1 human immunodeficiency virus(HIV-1 RNA) .Results Fifty-one samples were positive by NAT ,but negative by ELISA .A total of 33 samples in these 51 samples were identified .The HBV-DNA of 32 donors were positive ,and the HIV-1 RNA of one donor was positive ,who was identified as suspected HIV-1 positive (gp160 and p24 ± ) by western blot method in Nanjing municipal center for disease control and prevention . Six months later ,this blood donor was identified as HIV-1 positive (gp160 ,gp120 ,p66 ,p51 ,gp41 ,p31 ,p24 ,p17 + ) by western blot method in Nanjing municipal center for disease control and prevention .Conclusion NAT could effec-tively decrease the infection induced by blood donors in the window period and infected insidiously ,so as to reduce the risk of blood transfusion .%目的:采取核酸检测(NAT )技术进行血液筛查,减少由献血者感染“窗口期”及隐匿感染献血引起的疾病传播。方法采用酶联免疫吸附法(ELISA)对54358份献血者标本进行乙型肝炎表面抗原(HBsAg)、人类免疫缺陷病毒抗体(抗-HIV)、丙型肝炎病毒抗体(HCV-Ab)检测,同时用转录介导扩增技术(TMA)进行 HBV-DNA 、HCV-RNA 检测,人类免疫缺陷病毒1型(HIV-1)RNA 核酸检测。结果 NAT 检测阳性 ELISA 检测阴性标本共51份。51份标本有鉴别试验结果的33份,其中32份 HBV-DNA 阳性,1份 HIV-1 RNA

  20. Microfluidic Devices for Blood Fractionation

    Directory of Open Access Journals (Sweden)

    Chwee Teck Lim

    2011-07-01

    Full Text Available Blood, a complex biological fluid, comprises 45% cellular components suspended in protein rich plasma. These different hematologic components perform distinct functions in vivo and thus the ability to efficiently fractionate blood into its individual components has innumerable applications in both clinical diagnosis and biological research. Yet, processing blood is not trivial. In the past decade, a flurry of new microfluidic based technologies has emerged to address this compelling problem. Microfluidics is an attractive solution for this application leveraging its numerous advantages to process clinical blood samples. This paper reviews the various microfluidic approaches realized to successfully fractionate one or more blood components. Techniques to separate plasma from hematologic cellular components as well as isolating blood cells of interest including certain rare cells are discussed. Comparisons based on common separation metrics including efficiency (sensitivity, purity (selectivity, and throughput will be presented. Finally, we will provide insights into the challenges associated with blood-based separation systems towards realizing true point-of-care (POC devices and provide future perspectives.

  1. 40 CFR 52.1323 - Approval status.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 4 2010-07-01 2010-07-01 false Approval status. 52.1323 Section 52.1323 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED... on January 16, 1979 (44 FR 3274) are met. (b) The Administrator approves Rule 10 CSR 10-2.290...

  2. Obinutuzumab breaks through to FDA approval.

    Science.gov (United States)

    2014-01-01

    The U.S. Food and Drug Administration approved the monoclonal antibody obinutuzumab for use with chlorambucil in patients with previously untreated chronic lymphocytic leukemia. The drug is the first to receive approval under the agency's breakthrough therapy designation, created in July 2012.

  3. 46 CFR 154.1005 - Equipment approval.

    Science.gov (United States)

    2010-10-01

    ... carried. (b) Each submerged cargo pump motor installation must be specially approved by the Commandant (CG... FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Design, Construction and Equipment Electrical § 154.1005 Equipment approval. (a) Electrical equipment that is required to be intrinsically safe...

  4. 38 CFR 21.7220 - Course approval.

    Science.gov (United States)

    2010-07-01

    ...; Pub. L. 98-525) (b) Course approval criteria. In administering benefits payable under 38 U.S.C.... (1) Section 21.4250 (except paragraph (c)(1))—Jurisdiction for course and licensing and certification... study; and (12) Section 21.4268—Approval of licensing and certification tests. (Authority: 38...

  5. Application of fully automated blood grouping analyzer in the blood donor testing%全自动血型分析仪应用于献血者血型筛查

    Institute of Scientific and Technical Information of China (English)

    周国平; 周结; 向东; 谢云峥; 杨军; 郑岚; 曹斌; 吴蓉晖

    2011-01-01

    Objective To evaluate the performance of a fully automated blood grouping analyzer for ABO and RhD screening and the red blood cell IgM unexpected antibody detection. Methods A total of 25 554 samples were collected from blood donors. ABO, RhD, and IgM unexpected antibodies were tested simultaneously by fully automated blood grouping analyzer and manual colorimetric method with semi-automated sampler. For discrepancies between forward and reverse ABO grouping, agglutination of O cells, RhD negative results, samples would be referred to the reference laboratory of Shanghai Blood Center for further identification. Results The accuracy rates of ABO grouping by fully automated blood grouping analyzer and manual colorimetric method with semi-automated sampler were 99.93% (25 535/25 554) and 99.95% (25 542/25 554)respectively(P > 0.05 ) ;the rates of agglutination of O cells were 0.18% (46/25 554), and 0.10% (26/25 554) (P <0.05) respectively;the ABO forward and reverse grouping discrepancies were 17(0.06% ) and 10(0.04% ) ,respectively. Reference lab confirmed that there were 5 subgroups discovered by both methods ;2 subgroups were missed by each method(0.01% ) ,the rest were normal ABO blood group specimens (10/17 vs 3/10, P > 0.05). Conclusion The fully automated blood grouping analyzer can perform blood donor testing with high accuracy, high standardization in operation, and easier identification of IgM irregular antibodies.%目的 探讨并评价全自动血型分析仪应用于献血者血型筛查和盐水不规则抗体检测.方法 采用全自动血型分析仪(全自动法)对25 554例献血者标本作ABO及BhD血型鉴定、盐水不规则抗体初筛,并与加样仪加样手工比色法(半自动法)作比对实验.ABO正反定型不一致而无法定型、O细胞凝集、RhD阴性的标本送血型红细胞参比实验室鉴定.结果 全自动法与半自动法比较,ABO、RhD阴性血型1次准确定型率:99.93%(25 535/25 554)vs 99

  6. Efficacy and safety concerns are important reasons why the FDA requires multiple reviews before approval of new drugs.

    Science.gov (United States)

    Ross, Joseph S; Dzara, Kristina; Downing, Nicholas S

    2015-04-01

    The regulatory approval of new drugs by the Food and Drug Administration (FDA) is a long and complex process and often requires multiple cycles of review, potentially delaying patients' access to new and effective therapeutics. We used qualitative methods to characterize the safety and efficacy reasons why applications for novel therapeutics approved by the FDA between 2001 and 2011 required multiple review cycles prior to approval. Among ninety-six applications approved between 2001 and 2011 that required multiple review cycles, safety concerns contributed to seventy-four (77.1 percent) and efficacy concerns to forty-three (44.8 percent). Our study suggests that multiple review cycles appear to play an important role in allowing the FDA to protect public health and in ensuring adequate understanding of clinical benefits and risks prior to approval.

  7. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... Initiative Research Programs and Awards View all Blood Current Issue First Edition Abstracts Blood Advances A peer- ... Get email updates View all meetings Publications Blood Current Issue First Edition Abstracts Blood Advances A peer- ...

  8. Hypertension (High Blood Pressure)

    Science.gov (United States)

    ... Surgery? A Week of Healthy Breakfasts Shyness Hypertension (High Blood Pressure) KidsHealth > For Teens > Hypertension (High Blood Pressure) Print ... rest temperature diet emotions posture medicines Why Is High Blood Pressure Bad? High blood pressure means a person's heart ...

  9. White Blood Cell Count

    Science.gov (United States)

    ... limited. Home Visit Global Sites Search Help? White Blood Cell Count Share this page: Was this page helpful? ... Count; Leukocyte Count; White Count Formal name: White Blood Cell Count Related tests: Complete Blood Count , Blood Smear , ...

  10. Lead levels - blood

    Science.gov (United States)

    Blood lead levels ... A blood sample is needed. Most of the time blood is drawn from a vein located on the inside ... may be used to puncture the skin. The blood collects in a small glass tube called a ...

  11. Blood donation before surgery

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/patientinstructions/000367.htm Blood donation before surgery To use the sharing features on ... described here. Blood From the Public (Volunteer Blood Donation) The most common source of blood given during ...

  12. High Blood Cholesterol

    Science.gov (United States)

    ... version of this page please turn Javascript on. High Blood Cholesterol What is High Blood Cholesterol? What is Cholesterol? Cholesterol is a ... heart disease. If Your Blood Cholesterol Is Too High Too much cholesterol in your blood is called ...

  13. Low Blood Pressure

    Science.gov (United States)

    ... a problem. Sometimes blood pressure that is too low can also cause problems. Blood pressure is the ... reading is 90/60 or lower, you have low blood pressure. Some people have low blood pressure ...

  14. Blood Transfusion (For Parents)

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Blood Transfusions KidsHealth > For Parents > Blood Transfusions A A ... and help put your child at ease. About Blood Transfusions Blood is like the body's transportation system. ...

  15. 78 FR 76387 - Notice of Passenger Facility Charge (PFC) Approvals and Disapprovals

    Science.gov (United States)

    2013-12-17

    ...-2835. Public Agency: City of Garden City, Kansas. Application Number: 13-01-C-00-GCK. Application Type... Garden City Regional Airport. Brief Description of Projects Approved for Collection and Use: Security... lighting. Rehabilitate taxiway--phase I. Install runway vertical/visual guidance system....

  16. 15 CFR 923.133 - Procedure for conducting continuing reviews of approved State CZM programs.

    Science.gov (United States)

    2010-01-01

    ... will submit a financial assistance application or work program, whichever is applicable, on a timetable... evaluation team indicating that they wish to receive the final findings. Notice of the availability of the... approved CZM program; (B) The management agency is effectively playing a leadership role in coastal...

  17. 院外快速血糖检测在急性酒精中毒的应用%Application of fast blood glucose detection for acute alcohol intoxication patient In prehospital

    Institute of Scientific and Technical Information of China (English)

    班宇侠; 蒋小燕

    2012-01-01

    目的 探讨院外救治急性酒精中毒患者时,应用检测快速血糖的意义和必要性.方法 对108 例因"急性酒精中毒"呼叫120救护车的患者行快速血糖检测.结果 血糖正常者54例;血糖<2.8 mmol/L 者13例;血糖≧11.1 mmol/L 者36例;血糖结果提示"Hi测不出"者5例.结论 急性酒精中毒患者的血糖 复杂多变,院外救治中应常规检测血糖,对血糖变化异常患者给予正确的处理,为安全转运提供保障,更 为院内进一步系统治疗提供依据.%Objective To explore the application of fast blood glucose detection for acute alcohol intoxication patient in pre-hospital.Method 108 patients who called for 120 first aid ambulance for acute alcohol intoxication were analyzed. Result The normal blood glucose;in 108 cases were 54 cases,anb blood glucose<2.8 mmol/1 were 13 cases; blood glucose ≧ 11.1 mmol/lwere 36cases; and there 5 cases can' t be detected.. Conclusion The blood glucose of acute alcohol intoxication patient were complexity, blood detection should be used in prehospital rescue regularly, and give proper treatment to abnormal glucose patient for provide operational security, and offer a reference for systematical treatment in hospital.

  18. 75 FR 75955 - Notice of Request for Extension and Revision of a Currently Approved Information Collection for...

    Science.gov (United States)

    2010-12-07

    ... canned ripe olives, raisins, and dates which are required to be inspected by AMS, subject to exemptions... the regulations regarding all licensed samplers upon approval of the application. Estimate of...

  19. Can latent heat safely warm blood? – in vitro testing of a portable prototype blood warmer

    Directory of Open Access Journals (Sweden)

    McEwen Mark P

    2007-08-01

    Full Text Available Abstract Background Trauma/retrieval patients are often in shock and hypothermic. Treatment of such patients usually involves restoring their blood volume with transfusion of blood (stored at 2°C – 6°C and/or crystalloids or colloids (stored at ambient temperature. Rapid infusion of these cold fluids can worsen or even induce hypothermia in these patients. Warming of intravenous fluids at accident sites has traditionally been difficult due to a lack of suitable portable fluid warmers that are not dependent on mains electrical or battery power. If latent heat, the heat released when a liquid solidifies (an inherently temperature limiting process can warm intravenous fluids, portable devices without a reliance on electrical energy could be used to reduce the incidence of hypothermia in trauma patients. Methods Rapid infusion of red cells into patients was timed to sample typical clinical flow rates. An approved dry heat blood warmer was compared with a prototype blood warmer using a supercooled liquid latent heat storage material, to warm red cells whilst monitoring inlet and outlet temperatures. To determine the effect of warming on red cell integrity compared to the normal storage lesion of blood, extracellular concentrations of potassium, lactate dehydrogenase and haemoglobin were measured in blood which had been warmed after storage at 2°C – 6°C for 1 to 42 days. Results A prototype latent heat fluid warmer consistently warmed red cells from approximately 4°C to approximately 35°C at typical clinical flow rates. Warming of stored blood with latent heat did not affect red cell integrity more than the approved dry heat blood warmer. Conclusion Using latent heat as an energy source can satisfactorily warm cold blood or other intravenous fluids to near body temperature, without any adverse affects.

  20. [Changes of twenty-four-hour profile blood pressure and its correction of patients with arterial hypertension on the background of combined antihypertensive therapy application].

    Science.gov (United States)

    Solomennchuk, T M; Slaba, N A; Prots'ko, V V; Bedzaĭ, A O

    2014-01-01

    The aim of this research was the study of efficiency and endurance antihypertensive therapy on the basis of fixed combination of enalapril and hydrochlorothiazide (HCTZ) and enalapril and HCTZ in combination with amlodipine according to the twenty-four-hour (? day-and-night) monitoring of blood pressure (? 24H BPM) of patients with arterial hypertension (AH) 2-3 severity. The study included 33 patients with 2-3 grade of hypertension (average age--54,40 ± 3.45 years). All patients performed ? 24H BPM before treatment and after 12 weeks of therapy. The combination of enalapril and HCTZ allowed to achieve target levels of blood pressure in 79% of patients, amlodipine additional purpose--in 86% of patients. We found that this therapy has a corrective effect on daily blood pressure profile, significantly reducing the load pressure and blood pressure variability. During treatment with the combination of enalapril and HCTZ combination of enalapril, HCTZ with amlodipine optimal daily profile of blood pressure after 12 weeks of reaching respectively 63.1% and 71.4% of patients. The treatment with combination of enalapril and HCTZ and adding of amlodipine is characterized by good endurance and high adherence to treatment.

  1. Development and Validation of a GC-MS Method for the Detection and Quantification of Clotiapine in Blood and Urine Specimens and Application to a Postmortem Case

    Directory of Open Access Journals (Sweden)

    Giulio Mannocchi

    2015-01-01

    Full Text Available Introduction. Clotiapine is an atypical antipsychotic of the dibenzothiazepine class introduced in a few European countries since 1970, efficient in treatment-resistant schizophrenic patients. There is little published data on the therapeutic and toxic concentrations of this drug. Aims. The aim of the present study is the development and validation of a method that allows the detection and quantification of clotiapine in blood and urine specimens by gas chromatography-mass spectrometry (GC-MS. Methods. Validation was performed working on spiked postmortem blood and urine samples. Samples were extracted with liquid-liquid extraction (LLE technique at pH 8.5 with n-hexane/dichloromethane (85/15 v/v and analysis was followed by GC-MS. Methadone-d9 was used as internal standard. Results. The limit of detection (LOD was 1.2 and 1.3 ng/mL for urine and blood, respectively, while the lower limit of quantification (LLOQ was 3.9 and 4.3 ng/mL, respectively. Linearity, precision, selectivity, accuracy, and recovery were also determined. The method was applied to a postmortem case. The blood and urine clotiapine concentrations were 1.32 and 0.49 μg/mL, respectively. Conclusions. A reliable GC-MS method for the detection and quantification of clotiapine in blood and urine samples has been developed and fully validated and then applied to a postmortem case.

  2. Development and Validation of a GC-MS Method for the Detection and Quantification of Clotiapine in Blood and Urine Specimens and Application to a Postmortem Case

    Science.gov (United States)

    Mannocchi, Giulio; Pantano, Flaminia; Tittarelli, Roberta; Catanese, Miriam; Umani Ronchi, Federica; Busardò, Francesco Paolo

    2015-01-01

    Introduction. Clotiapine is an atypical antipsychotic of the dibenzothiazepine class introduced in a few European countries since 1970, efficient in treatment-resistant schizophrenic patients. There is little published data on the therapeutic and toxic concentrations of this drug. Aims. The aim of the present study is the development and validation of a method that allows the detection and quantification of clotiapine in blood and urine specimens by gas chromatography-mass spectrometry (GC-MS). Methods. Validation was performed working on spiked postmortem blood and urine samples. Samples were extracted with liquid-liquid extraction (LLE) technique at pH 8.5 with n-hexane/dichloromethane (85/15 v/v) and analysis was followed by GC-MS. Methadone-d9 was used as internal standard. Results. The limit of detection (LOD) was 1.2 and 1.3 ng/mL for urine and blood, respectively, while the lower limit of quantification (LLOQ) was 3.9 and 4.3 ng/mL, respectively. Linearity, precision, selectivity, accuracy, and recovery were also determined. The method was applied to a postmortem case. The blood and urine clotiapine concentrations were 1.32 and 0.49 μg/mL, respectively. Conclusions. A reliable GC-MS method for the detection and quantification of clotiapine in blood and urine samples has been developed and fully validated and then applied to a postmortem case. PMID:26236337

  3. 灰色GM(1.1)模型在预测临床用血需求量中的应用%Application of Gray GM(1.1)Model in forecasting clinic blood requirement

    Institute of Scientific and Technical Information of China (English)

    王晓军; 付超; 孙琳

    2011-01-01

    Objective To guarantee blood resources for reasonable clinic usage and provide basis for blood collection plan in a scientific way.Methods We applied statistical methods in forecasting clinic blood requirement and made statistical analysis of clinic blood usage volumes in Chengdu City from 2002 to 2009, and established the gray GM (1.1) model to forecast the clinic blood usage volumes of 2010, 2011 and 2012.Results The clinic blood requirement volumes for 2010, 2011 and 2012 are respectively 195568, 229611 and 270089 units.Conclusion The gray GM (1.1) model has such advantages as a smaller quantity of samples required, no need for typical probability distribution, easy calculation and a high degree of fitting.Therefore, it is an applicable tool for blood banks to make plans of blood collection, preparation and provision and to enhance scientific management.%目的 为保障临床合理利用血液资源,科学制定血液采集计划提供依据.方法 将统计学方法应用于临床用血需求量预测中,对成都市2002~2009年临床用血量进行统计分析,建立灰色GM(1、1)模型,预测2010、2011、2012年临床用血需求量.结果 2010、2011、2012年临床用血需求量分别为195568、229611、270089单位.结论 灰色GM(1.1)模型具有所需样本量小,无需典型概率分布,计算简便和拟合度高等优点,是血站制定血液采集、制备、供应计划,强化科学管理的可行性工具.

  4. 壮医气血理论的理论基础与临床应用%Theoretical foundation and clinical application of the qi and blood theory of Zhuang Medicine

    Institute of Scientific and Technical Information of China (English)

    宋宁

    2013-01-01

    壮医认为气和血是构成人体和涵养生命的最基本物质,二者通过三道两路系统而循环流注全身,涵养机体组织,推动人体生理功能协调运行.气血宜动、宜衡,又相互资生,相依为用,气血平衡调畅,则人体健康;气血失衡,则产生疾病.气血理论是壮医核心理论,也是壮医重要的病机理论,对指导临床实践具有重要价值.文章阐述了壮医气血的概念、内涵及其关系,气血的生理功能、病理变化及病机特点,壮医气血理论的内容及其应用,提出平衡气血治则治法,初步建立壮医的理法方药体系.%Zhuang Medicine considered that qi and blood are the most basic elements which constitute human body and nourish life. Qi and blood circulate in the whole human body and nourish life which can promote physiological function by the system of three paths and two passages. Qi and blood should be better to move and keep harmony; both of them rely on each other. If they keep harmony, the body can keep health, or otherwise disharmony may result in some diseases. In this point, Qi and blood theory is the core of Zhuang Medicine which is the important pathogenesis theory and offers a significant guideline to the clinical practice. This paper described the definition, connotation and relationship between qi and blood in Zhuang Medicine. It also illustrated the physiological function, pathological changes and pathogenesis characteristics of qi and blood, as well as the content of Qi and blood theory and its applications. It also puts forward to the therapeutic principle and method of keeping balance between qi and blood, which has a preliminary establishment of the system of Zhuang Medicine in its theory, method, formula and herbs.

  5. Test for antioxidant ability by scavenging long-lived mutagenic radicals in mammalian cells and by blood test with intentional radicals: an application of gallic acid

    Science.gov (United States)

    Kumagai, Jun; Kawaura, Tomoko; Miyazaki, Tetsuo; Prost, Michel; Prost, Emmanuelle; Watanabe, Masami; Quetin-Leclercq, Joëlle

    2003-01-01

    Antioxidant ability of gallic acid (GA) are determined both by electron spin resonance measurement of long-lived radicals produced in γ-ray irradiated Syrian golden hamster embryo cells with GA and by hemolysis measurement with GA when blood cells are submitted to radicals. Scavenging properties of GA are determined by the reaction rate constant with long-lived mutagenic radicals in the cells while the blood test allows to analyze the global effects of this compound: radical scavenger+metal ion chelator+regeneration of intra- and extra-cellular antioxidant.

  6. Compact NMR relaxometry of human blood and blood components.

    Science.gov (United States)

    Cistola, David P; Robinson, Michelle D

    2016-11-01

    Nuclear magnetic resonance relaxometry is a uniquely practical and versatile implementation of NMR technology. Because it does not depend on chemical shift resolution, it can be performed using low-field compact instruments deployed in atypical settings. Early relaxometry studies of human blood were focused on developing a diagnostic test for cancer. Those efforts were misplaced, as the measurements were not specific to cancer. However, important lessons were learned about the factors that drive the water longitudinal (T1) and transverse (T2) relaxation times. One key factor is the overall distribution of proteins and lipoproteins. Plasma water T2 can detect shifts in the blood proteome resulting from inflammation, insulin resistance and dyslipidemia. In whole blood, T2 is sensitive to hemoglobin content and oxygenation, although the latter can be suppressed by manipulating the static and applied magnetic fields. Current applications of compact NMR relaxometry include blood tests for candidiasis, hemostasis, malaria and insulin resistance.

  7. Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications

    Directory of Open Access Journals (Sweden)

    Wim van Oeveren

    2012-01-01

    We concluded that the Hemobile minimally affects blood and could be adjusted to high blood flows, simulating arterial shear stress. The Hemobile was used to measure hemocompatibility of graft material and showed Dyneema Purity UHMWPE fiber in many ways more hemocompatible than ePTFE and PET.

  8. The application value of blood purification in the treatment of acute renal injury%血液净化在急性肾损伤治疗中的应用价值探讨

    Institute of Scientific and Technical Information of China (English)

    苗小梅; 马文录

    2015-01-01

    Objective acute kidney injury (AKI) is an abnormality of the kidney function or structure that is not more than 3 months. The incidence of the year increased year by year, Methods the treatment method is mainly blood purification therapy and drug treatment, is still based on blood purification therapy. Results in this paper, we used the method of hemodialysis, blood dialysis and other methods to treat acute renal injury, and to explore the application value of blood purification in the treatment of acute renal injury. Conclusion This article discusses 39 cases of patients treated with 2 years of purification in our hospital, to explore the value of the use of blood purification treatment for acute renal injury.%目的:急性肾损伤(AKI)是指不超过3个月的肾脏功能或结构方面的异常。其发生率逐年增高,方法治疗方法主要是血液净化疗法和药物治疗,目前仍以血液净化疗法为主。结果该文就采用其中的血液透析、血液透析滤过等方法来治疗急性肾损伤,结论并血液净化在急性肾损伤治疗中的应用价值探讨。本文探讨了本院近2年净化治疗患者39例,来探讨急性肾损伤使用血液净化治疗的价值。

  9. Application and Nursing of Venous Indwelling Needle in Drug Clinical Trial Blood Sampling%静脉留置针在药物临床试验采血中的应用与护理

    Institute of Scientific and Technical Information of China (English)

    于伟越; 袁永清; 王玲; 王华光

    2013-01-01

    Objective To discuss the applicability of venous indwelling needle in drug clinical trial blood sampling .Methods A to-tal of 158 patients use vein needle to draw blood in phase Ⅰ clinical trial according to the standard procedures .To observe blood sam-pling effect and the suitability and safety of participants .Results We successfully completed the clinical test collection work under the condition of vein needle average retention time which is one day .Subjects had no obvious discomfort chief complaint .Only 3 cases of subjects had puncture blood ,6 cases had blocking pipe .No subject get infection .Conclusion It is effective and safe that using venous indwelling needle in continuous blood sampling of phase Ⅰ clinical trial .%  目的探讨静脉留置针在药物临床试验采血中的适用性。方法在进行Ⅰ药物临床试验(phase Ⅰ clinical trial)时,对158例受试者使用静脉留置针进行采血,按照标准操作规程进行操作,观察采血效果及受试者的适宜性和安全性。结果在静脉留置针平均保留时间为1天的情况下,顺利完成了临床试验的采血工作,受试者无明显不适主诉,仅有3例出现穿刺部位渗血,6例发生堵管,无一例发生感染。结论静脉留置针用于I期药物临床试验的连续采血是有效和安全的。

  10. Application of PDCA cycle management method in reducing false positive rate of neonatal blood culture%PDCA 循环管理法在降低新生儿血培养假阳性率中的应用

    Institute of Scientific and Technical Information of China (English)

    闵凡云; 周传銮

    2013-01-01

    目的:探讨PDCA循环管理法在降低新生儿血培养假阳性率中的应用及临床效果,为指导临床诊断及合理应用抗菌药物提供客观依据。方法:对2010年7~12月新生儿科32例血培养阳性患儿进行目标追踪监测,了解分析假阳性发生原因并采取PDCA循环管理法,比较实施前后新生儿血培养假阳性率。结果:实施前血培养假阳性患儿病原菌分布以条件致病菌为主,实施前后血培养假阳性率比较差异有统计学意义( P<0.01)。结论:将PDCA循环管理法应用于新生儿血培养阳性目标性监测,可提高血培养标本送检质量,降低假阳性率,保证检验结果准确。%Objective:To explore the application of PDCA cycle management method in reducing false positive rate of neonatal blood culture and investigate the clinical effect in order to provide objective basis for guiding clinical diagnosis and reasonable application of anti -biotics.Methods:The target tracking monitoring was conducted to 32 newborns(born from July to December 2010) whose blood culture was positive to understand and analyze the false positive causes ,and the PDCA cycle management method was implemented .The false positive rate of neonatal blood culture was compared before and after the implementation of the PDCA cycle management method .Results:The con-ditionally pathogenic bacteria in the false positive blood culture was the main pathogen in the distribution before the implementation of the method;the difference in the comparison of the false positive rate of blood culture was statistically significant before and after the imple -mentation of the method(P<0.01).Conclusion:The PDCA cycle management method applied to the target tracking monitoring can im-prove the quality of blood culture specimens and reduce the false positive rate so as to ensure accurate test results .

  11. 全自动血培养系统在外伤性眼内炎的临床应用%Clinical application of automated blood culture system in traumatic endophthalmitis

    Institute of Scientific and Technical Information of China (English)

    任玉玲; 薛黎萍; 刘春林; 肖丽波

    2012-01-01

    Objective To evaluate the clinical application of BacT/ALERT 3D automated blood culture system in traumatic endophthalmitis.Methods A total of 113 vitreous specimens of traumatic endophthalmitis were collected by BacT/ALERT PF pediatric blood culture bottle,detected by BacT/ALERT 3D automated blood culture system and analysed by Vitek-2 compact.We evaluated the positive rate,time to show positive,the types of microorganisms.Results In a total of 113 cultured vitreous specimens,81 cases were positive.Positive rate was 71.7%.Grampositive microorganisms accounted for 62.9% of the positive culture results.Gramnegative microorganisms accounted for 34.6% and fungi accounted for 2.5%.The shortest time to show positive was 4h.Conclusion The automated blood culture system has been widely used in blood and body fluid culture.The application of the automated blood culture for endophthalmitis can raise the positive detection rate,reduce the detection time to show positive and increase the types of microorganisms detected.%目的 评价全自动血培养系统( BacT/ALERT 3 D)在外伤性眼内炎的临床应用.方法 外伤性眼内炎113例(113眼)的玻璃体液进行病原菌培养,用中和抗生素儿童培养瓶( BacT/A-LERT PF)采集标本,全自动血培养系统进行病原菌培养,Vitek-2 compact进行菌种鉴定和药敏试验,对检出阳性者,分析阳性率和病原菌种类.结果 113份玻璃体液标本检出病原微生物81例,阳性率为71.7%,其中革兰阳性细菌占62.9%,革兰阴性细菌占34.6%,真菌占2.5%.最快检出时间为4h.结论 全自动血培养系统应用于外伤性眼内炎提高了玻璃体液病原菌培养的阳性率,缩短阳性检出时间,检出病原菌种类多,结果准确.

  12. Noninvasive near-infrared blood glucose monitoring using a calibration model built by a numerical simulation method: Trial application to patients in an intensive care unit.

    Science.gov (United States)

    Maruo, Katsuhiko; Oota, Tomohiro; Tsurugi, Mitsuhiro; Nakagawa, Takehiro; Arimoto, Hidenobu; Hayakawa, Mineji; Tamura, Mamoru; Ozaki, Yukihiro; Yamada, Yukio

    2006-12-01

    We have applied a new methodology for noninvasive continuous blood glucose monitoring, proposed in our previous paper, to patients in ICU (intensive care unit), where strict controls of blood glucose levels are required. The new methodology can build calibration models essentially from numerical simulation, while the conventional methodology requires pre-experiments such as sugar tolerance tests, which are impossible to perform on ICU patients in most cases. The in vivo experiments in this study consisted of two stages, the first stage conducted on healthy subjects as preliminary experiments, and the second stage on ICU patients. The prediction performance of the first stage was obtained as a correlation coefficient (r) of 0.71 and standard error of prediction (SEP) of 28.7 mg/dL. Of the 323 total data, 71.5% were in the A zone, 28.5% were in the B zone, and none were in the C, D, and E zones for the Clarke error-grid analysis. The prediction performance of the second stage was obtained as an r of 0.97 and SEP of 27.2 mg/dL. Of the 304 total data, 80.3% were in the A zone, 19.7% were in the B zone, and none were in the C, D, and E zones. These prediction results suggest that the new methodology has the potential to realize a noninvasive blood glucose monitoring system using near-infrared spectroscopy (NIRS) in ICUs. Although the total performance of the present monitoring system has not yet reached a satisfactory level as a stand-alone system, it can be developed as a complementary system to the conventional one used in ICUs for routine blood glucose management, which checks the blood glucose levels of patients every few hours.

  13. Simultaneous analysis of six amphetamines and analogues in hair, blood and urine by LC-ESI-MS/MS. Application to the determination of MDMA after low ecstasy intake.

    Science.gov (United States)

    Chèze, Marjorie; Deveaux, Marc; Martin, Claire; Lhermitte, Michel; Pépin, Gilbert

    2007-08-06

    A rapid and sensitive method using LC-MS/MS triple stage quadrupole for the determination of traces of amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy"), 3,4-methylenedioxyethamphetamine (MDEA), and N-methyl-1-(3,4-methylenedioxyphenyl)-2-butanamine (MBDB) in hair, blood and urine has been developed and validated. Chromatography was carried out on an Uptisphere ODB C(18) 5 microm, 2.1 mm x 150 mm column (Interchim, France) with a gradient of acetonitrile and formate 2 mM pH 3.0 buffer. Urine and blood were extracted with Toxitube A (Varian, France). Segmented scalp hair was treated by incubation 15 min at 80 degrees C in NaOH 1M before liquid-liquid extraction with hexane/ethyl acetate (2/1, v/v). The limits of quantification (LOQ) in blood and urine were at 0.1 ng/mL for all analytes. In hair, LOQ was urine; in the range 5-500 pg/mg for MA, MDMA, MDEA and MBDB, and 20-500 pg/mg for AP and MDA. Inter-day precisions were urine at 1 and 50 ng/mL and urine was collected a few hours after (T+12h) and tested positive to amphetamines by immunoassay by a clinical laboratory. Blood and urine were sampled for forensic purposes at day 8 (D+8) and scalp hair at day 60 (D+60). No MDMA was detected in blood, but urine and hair were tested positive, respectively at 0.42 ng/mL and at 22 pg/mg in hair only in the segment corresponding to the period of the offence, while no MDA was detectable. This method allows the detection of MDMA up to 8 days in urine after single intake.

  14. Liquid chromatography-electrospray mass spectrometry determination of ibogaine and noribogaine in human plasma and whole blood. Application to a poisoning involving Tabernanthe iboga root.

    Science.gov (United States)

    Kontrimaviciūte, Violeta; Breton, Hélène; Mathieu, Olivier; Mathieu-Daudé, Jean-Claude; Bressolle, Françoise M M

    2006-11-07

    A liquid chromatography/electrospray ionization mass spectrometry (LC-ESI-MS) method was developed for the first time for the determination of ibogaine and noribogaine in human plasma and whole blood. The method involved solid phase extraction of the compounds and the internal standard (fluorescein) from the two matrices using OasisHLB columns. LC separation was performed on a Zorbax eclipse XD8 C8 column (5 microm) with a mobile phase of acetonitrile containing 0.02% (v/v) trimethylamine and 2mM ammonium formate buffer. MS data were acquired in single ion monitoring mode at m/z 311.2, 297.2 and 332.5 for ibogaine, noribogaine and fluorescein, respectively. The drug/internal standard peak area ratios were linked via a quadratic relationship to plasma (0.89-179 microg/l for ibogaine; 1-200 microg/l for noribogaine) and to whole blood concentrations (1.78-358 microg/kg for ibogaine; 2-400 microg/kg for noribogaine). Precision ranged from 4.5 to 13% and accuracy was 89-102%. Dilution of the samples had no influence on the performance of the method. Extraction recoveries were > or =94% in plasma and > or =57% in whole blood. The lower limits of quantitation were 0.89 microg/l for ibogaine and 1 microg/l for noribogaine in plasma, and 1.78 microg/kg for ibogaine and 2 microg/kg for noribogaine in whole blood. In frozen plasma samples, the two drugs were stable for at least 1 year. In blood, ibogaine and noribogaine were stable for 4h at 4 degrees C and 20 degrees C and 2 months at -20 degrees C. The method was successfully used for the analysis of a poisoning involving Tabernanthe iboga root.

  15. HS-SPME-GC/MS法在血液气味分析中的应用%Application of HS-SPME-GC/MS in analysis of blood odor

    Institute of Scientific and Technical Information of China (English)

    王辛; 龙成生; 吴德华; 强京宁; 张汇东; 单军

    2012-01-01

    A method for determination of odorants in human blood had been developed with Gas Chro-matography-Mass Spectrometry (GC-MS) combined with Head Space Solid Phase Micro-Extraction (HS-SPME), and the extraction conditions of SPME were optimized. Nine compounds in human bloods were I-dentified and then used to compare the blood odor profiles. These compounds were allyl methyl sulfide, cyclohexanol, 2-ethyl hexanoic acid, 3,7-dimethyl-6-octen-l-ol, 2-n-octyl-cyclepentene, 2-methyl-3-decen-5-one, ethyl 2-hexenoate, 2-methyl butanal, tributyl phosphate. The results demonstrated that there was difference in blood odors between humans and animals. The origins of bloods might be determined based on their odor profiles.%建立了顶空固相微萃取-气相色谱质谱联用检测血液气味的方法,优化了固相微萃取条件,对人、鸡和犬血液气味的差异性进行了比较研究.以人体血液气味中存在的甲基烯丙基硫醚、2-乙基己酸、香茅醇、3-辛基环戊烯、2-甲基-3-葵烯-5-酮、2-己烯酸乙酯和磷酸三丁酯等9种化合物作为目标化合物进行比较分析.结果表明,人和动物血液气味的化学组成存在显著差异性,利用血液气味的差异性能够区分不同来源的血迹.

  16. Application of Whole Blood C-reactive Protein and White Blood Cell Count in Children With Pneumonia%全血C-反应蛋白与白细胞计数联合检测在小儿肺炎中的应用

    Institute of Scientific and Technical Information of China (English)

    温朝辉

    2016-01-01

    Objective To analyze the application of the detection of whole blood C-reactive protein and white blood cell count in infantile pneumonia.Methods 120 cases of children with pneumonia in our hospital from August 2013 to July 2015 were selected, there were 70 cases of bacterial bronchopneumonia and 50 cases of viral pneumonia among them. To selected another 60 healthy cases, to compare the whole blood C-reactive protein and white blood cell count about two groups.ResultsThe whole blood C-reactive protein and white blood cell count of the children with bacterial bronchopneumonia was higher than the children with viral pneumonia and the healthy cases, the difference was statistically significant (P<0.05). The whole blood C-reactive protein and white blood cell count of the children with the viral pneumonia was signiifcantly was higher than the healthy cases, the difference was statistically signiifcant (P<0.05).Conclusion The combination of whole blood C- reactive protein and white blood cell count can identify the type of pneumonia in children with pneumonia, and can detect and conifrm the bacterial bronchopneumonia faster.%目的:分析全血C-反应蛋白与白细胞计数联合检测在小儿肺炎中的应用。方法选取2013年8月~至2015年7月本院收治的肺炎患儿120例,其中细菌性支气管肺炎70例,病毒性肺炎50例,另再选取60例健康儿童,观察比较三组患儿的全血C-反应蛋白与白细胞计数。结果细菌性支气管肺炎患儿的全血C-反应蛋白和白细胞计数显著高于病毒性肺炎及健康儿童,差异具有统计学意义(P<0.05);病毒性肺炎患儿的全血C-反应蛋白与白细胞计数高于健康儿童,差异有统计学意义(P<0.05)。结论全血C-反应蛋白与白细胞计数联合检测在小儿肺炎中可以鉴别肺炎类型,能更快的检测及确诊出细菌性支气管肺炎。

  17. Drugs Approved for Colon and Rectal Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for use in colon cancer and rectal cancer. The list includes generic names, brand names, and common drug combinations, which are shown in capital letters.

  18. Drugs Approved for Gestational Trophoblastic Disease

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for gestational trophoblastic disease. The list includes generic names and brand names. The drug names link to NCI's Cancer Drug Information summaries.

  19. Drugs Approved for Head and Neck Cancer

    Science.gov (United States)

    This page lists cancer drugs approved by the Food and Drug Administration (FDA) for head and neck cancer. The list includes generic names and brand names. The drug names link to NCI’s Cancer Drug Information summaries.

  20. FDA Approves First Immunotherapy for Lymphoma

    Science.gov (United States)

    The FDA has approved nivolumab (Opdivo®) for the treatment of patients with classical Hodgkin lymphoma whose disease has relapsed or worsened after receiving an autologous hematopoietic stem cell transplantation followed by brentuximab vedotin (Adcetris®)

  1. Depression: FDA-Approved Medications May Help

    Science.gov (United States)

    ... Products For Consumers Home For Consumers Consumer Updates Depression: FDA-Approved Medications May Help Share Tweet Linkedin ... symptoms in some people. back to top Diagnosing Depression Diagnosis—which should be from a health care ...

  2. 30 CFR 7.409 - Approval marking.

    Science.gov (United States)

    2010-07-01

    ... approval number in addition to the number and size (gauge) of conductors and cable type. For cables containing electric conductors, the marking shall also include the voltage rating. For splices, the...

  3. Drugs Approved for Kidney (Renal Cell) Cancer

    Science.gov (United States)

    ... 2015 2014 2013 2012 Media Resources Media Contacts Multicultural Media ... This page lists cancer drugs approved by the Food and Drug Administration (FDA) for kidney (renal cell) cancer. The list ...

  4. Drugs Approved for Soft Tissue Sarcoma

    Science.gov (United States)

    ... 2015 2014 2013 2012 Media Resources Media Contacts Multicultural Media ... This page lists cancer drugs approved by the Food and Drug Administration (FDA) for soft tissue sarcoma. The list includes ...

  5. Drugs Approved for Non-Hodgkin Lymphoma

    Science.gov (United States)

    ... 2015 2014 2013 2012 Media Resources Media Contacts Multicultural Media ... This page lists cancer drugs approved by the Food and Drug Administration (FDA) for non-Hodgkin lymphoma. The list includes ...

  6. Optimization of a high-throughput whole blood expression profiling methodology and its application to assess the pharmacodynamics of interferon (IFN beta-1a or polyethylene glycol-conjugated IFN beta-1a in healthy clinical trial subjects

    Directory of Open Access Journals (Sweden)

    Allaire Normand E

    2013-01-01

    Full Text Available Abstract Background Clinical trials offer a unique opportunity to study human disease and response to therapy in a highly controlled setting. The application of high-throughput expression profiling to peripheral blood from clinical trial subjects could facilitate the identification of transcripts that function as prognostic or diagnostic markers of disease or treatment. The paramount issue for these methods is the ability to produce robust, reproducible, and timely mRNA expression profiles from peripheral blood. Single-stranded complementary DNA (sscDNA targets derived from whole blood exhibit improved detection of transcripts and reduced variance as compared to their complementary RNA counterparts and therefore provide a better option for interrogation of peripheral blood on oligonucleotide arrays. High-throughput microarray technologies such as the high-throughput plate array platform offer several advantages compared with slide- or cartridge-based arrays; however, manufacturer’s protocols do not support the use of sscDNA targets. Results We have developed a highly reproducible, high-through put, whole blood expression profiling methodology based on sscDNA and used it to analyze human brain reference RNA and universal human reference RNA samples to identify experimental conditions that most highly correlated with a gold standard quantitative polymerase chain reaction reference dataset. We then utilized the optimized method to analyze whole blood samples from healthy clinical trial subjects treated with different versions of interferon (IFN beta-1a. Analysis of whole blood samples before and after treatment with intramuscular [IM] IFN beta-1a or polyethylene glycol-conjugated IFN (PEG-IFN beta-1a under optimized experimental conditions demonstrated that PEG-IFN beta-1a induced a more sustained and prolonged pharmacodynamic response than unmodified IM IFN beta-1a. These results provide validation of the utility of this new methodology and

  7. Blood vessels, circulation and blood pressure.

    Science.gov (United States)

    Hendry, Charles; Farley, Alistair; McLafferty, Ella

    This article, which forms part of the life sciences series, describes the vessels of the body's blood and lymphatic circulatory systems. Blood pressure and its regulatory systems are examined. The causes and management of hypertension are also explored. It is important that nurses and other healthcare professionals understand the various mechanisms involved in the regulation of blood pressure to prevent high blood pressure or ameliorate its damaging consequences.

  8. Application of the 'gate effect' of a molecularly imprinted polymer grafted on an electrode for the real-time sensing of heparin in blood.

    Science.gov (United States)

    Yoshimi, Yasuo; Sato, Kuniaki; Ohshima, Masaki; Piletska, Elena

    2013-09-07

    Heparin is the most important anticoagulant drug used during surgeries and extracorporeal therapies. Although the blood levels of heparin should be monitored continuously during the procedure to ensure the safety of the patient, there is currently no technique for measuring heparin in real time. This study describes the use of a molecularly imprinted polymer (MIP) as a recognition element in the development of a heparin sensor for real-time monitoring. An indium tin oxide (ITO) electrode grafted with a heparin-specific MIP was used as a working electrode to perform cyclic voltammetry of ferrocyanide. The anodic current was found to be dependent on heparin concentration, probably due to the "gate effect", which is a change in the accessibility of the MIP-modified electrode to ferrocyanide, triggered by specific interaction between MIP and heparin. The kinetics of heparin interaction with the MIP-grafted electrode was evaluated using potentiostatic chronoamperometry of ferrocyanide in an electrochemical flow cell. The response time to stepwise changes in heparin concentration between 0 and 0.04 units per mL was estimated at 20 s, which is remarkably shorter than that achieved using conventional methods for monitoring heparin. The MIP-grafted electrode demonstrated exceptional sensitivity and could detect heparin in whole blood samples (0-6 units per mL) diluted 100-fold with physiological saline containing ferrocyanide. Therefore, the MIP-grafted electrode is suitable for real-time monitoring of heparin in blood. Another advantage is that a very small volume of blood is needed, which is very important, especially when regular measurements are required.

  9. 36 CFR 28.15 - Approval of local zoning ordinances.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Approval of local zoning... INTERIOR FIRE ISLAND NATIONAL SEASHORE: ZONING STANDARDS Federal Standards and Approval of Local Ordinances § 28.15 Approval of local zoning ordinances. (a) The Secretary shall approve local ordinances...

  10. 血液净化疗法在急性中毒中的应用%Application of blood purification therapy in acute poisoning

    Institute of Scientific and Technical Information of China (English)

    张兴国

    2012-01-01

    Blood purification therapy is a medical technique that has developed rapidly in recent years and has been widely used in many branches of clinical field. It can not only well clean out internal metabolic wastes, but may also eliminate the xog-enous toxicants, that has gradually become one of the best therapy measures for acute poisoning. The paper will specially introduce the elimination effect on various toxicants by different blood purification modes, the correlated factor of purification effect, and also discuss the detailed aspect on the selection of blood purification modes.%血液净化技术是近年来发展较为迅速的一项医疗技术,其应用范围已涉及到临床多个学科.该技术不仅可以清除体内代谢废物,还可以清除外源性毒物,已逐渐成为临床抢救急性重度中毒的首选的治疗手段之一.本文重点介绍了不同的血液净化方式对各种毒物的清除效果、影响血液净化疗效的相关因素,并就急性中毒中如何选择合适的血液净化方式等进行了较细致讨论.

  11. Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp. in Sheep and Goat Blood Samples from Pakistan

    Directory of Open Access Journals (Sweden)

    A Rasul

    2013-06-01

    Full Text Available Background: The present study was designed to detect the presence of tick-borne parasites (Theileria and Babesia spp. in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan.Methods: Reverse line blot (RLB assay was applied for the parasitic detection by the amplification of hypervariable V4 region of the 18S ribosomal RNA (rRNA gene. A membrane with covalently linked generic and species specific oligonucleotide probes was used for the hybridization of amplified PCR products.Results: Parasites were detected in 16% of the ruminant blood samples under study. Two Theileria species, T. lestoquardi and T. ovis, were identified in samples. 25, of the total 32, infected animals were from Khyber Pukhtoon Khwa.Conclusion: Sheep were more prone to tick borne haemoprotozans as 81% infected samples were sheep as compared to 19% goats (P > 0.001. Risk factor analysis revealed that male (P = 0.03, ani­mals infested by ticks (P = 0.03 and herd composed of sheep only (P = 0.001 were more infected by blood parasites.

  12. [Application of wavelet transform on improving detecting precision of the non-invasive blood components measurement based on dynamic spectrum method].

    Science.gov (United States)

    Li, Gang; Men, Jian-Long; Sun, Zhao-Min; Wang, Hui-Quan; Lin, Ling; Tong, Ying; Zhang, Bao-Ju

    2011-02-01

    Time-varying noises in spectra collection process have influence on the prediction accuracy of quantitative calibration in the non-invasive blood components measurement which is based on dynamic spectrum (DS) method. By wavelet transform, we focused on the absorbance wave of fingertip transmission spectrum in pulse frequency band. Then we increased the signal to noise ratio of DS data, and improved the detecting precision of quantitative calibration. After carrying out spectrum data continuous acquisition of the same subject for 10 times, we used wavelet transform de-noising to increase the average correlation coefficient of DS data from 0.979 6 to 0.990 3. BP neural network was used to establish the calibration model of subjects' blood components concentration values against dynamic spectrum data of 110 volunteers. After wavelet transform de-noising, the correlation coefficient of prediction set increased from 0.677 4 to 0.846 8, and the average relative error was decreased from 15.8% to 5.3%. Experimental results showed that the introduction of wavelet transform can effectively remove the noise in DS data, improve the detecting precision, and accelerate the development of non-invasive blood components measurement based on DS method.

  13. Blood Culture (For Parents)

    Science.gov (United States)

    ... Feeding Your 1- to 2-Year-Old Blood Culture KidsHealth > For Parents > Blood Culture Print A A ... adjust the treatment choice. Why Do a Blood Culture? During some illnesses, certain infection-causing bacteria and ...

  14. Coughing up blood

    Science.gov (United States)

    ... gastrointestinal tract. Blood that comes up with a cough often looks bubbly because it is mixed with ... conditions, diseases, and medical tests may make you cough up blood. These include: Blood clot in the ...

  15. High Blood Pressure

    Science.gov (United States)

    ... normal blood pressure 140/90 or higher is high blood pressure Between 120 and 139 for the top number, ... prehypertension. Prehypertension means you may end up with high blood pressure, unless you take steps to prevent it. High ...

  16. High Blood Pressure (Hypertension)

    Science.gov (United States)

    ... Print Page Text Size: A A A Listen High Blood Pressure (Hypertension) Nearly 1 in 3 American adults has ... weight. How Will I Know if I Have High Blood Pressure? High blood pressure is a silent problem — you ...

  17. What Is Blood?

    Science.gov (United States)

    ... Foundation for America's Blood Centers ADRP What is blood? PUBLICATIONS EDUCATION PRESS ROOM BLOG CAREERS CONTACT ABC ... for patients who need it. One unit of blood can be separated into the following components: Nearly ...

  18. Ketones blood test

    Science.gov (United States)

    ... Ketones - serum; Nitroprusside test; Ketone bodies - serum; Ketones - blood ... A blood sample is needed. ... When the needle is inserted to draw blood, some people feel slight ... there may be some throbbing or a slight bruise. This soon ...

  19. Magnesium blood test

    Science.gov (United States)

    Magnesium - blood ... A blood sample is needed. ... When the needle is inserted to draw blood, some people feel slight pain. Others feel a prick or stinging. Afterward, there may be some throbbing or a slight bruise. This soon ...

  20. CEA blood test

    Science.gov (United States)

    Carcinoembryonic antigen blood test ... A blood sample is needed . ... When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging sensation. Afterward, there may be some throbbing or a slight bruise. ...

  1. Glucagon blood test

    Science.gov (United States)

    ... type I - glucagon test; Hypoglycemia - glucagon test; Low blood sugar - glucagon test ... A blood sample is needed . ... When the needle is inserted to draw blood, some people feel ... Afterward, there may be some throbbing or a slight bruise. This ...

  2. Special Blood Donation Procedures

    Science.gov (United States)

    ... takes about 10 minutes. Double red blood cell donation In the so-called double red blood cell ... can be cured with apheresis. Directed or designated donation Family members or friends can donate blood specifically ...

  3. Home blood sugar testing

    Science.gov (United States)

    Diabetes - home glucose testing; Diabetes - home blood sugar testing ... Usual times to test your blood sugar are before meals and at bedtime. Your provider may ask you to check your blood sugar 2 hours after a meal. Ask ...

  4. High blood sugar

    Science.gov (United States)

    ... High blood glucose - self-care; Diabetes - high blood sugar ... Symptoms of high blood sugar can include: Being very thirsty or having a dry mouth Having blurry vision Having dry skin Feeling weak or tired ...

  5. Hyperglycemia (High Blood Glucose)

    Medline Plus

    Full Text Available ... Blood Pressure Physical Activity High Blood Glucose My Health Advisor Tools To Know Your Risk Alert Day ... DKA (Ketoacidosis) & Ketones Kidney Disease (Nephropathy) Gastroparesis Mental Health Step On Up Treatment & Care Blood Glucose Testing ...

  6. Blood and Diversity

    Science.gov (United States)

    ... patient diversity. For example, U-negative and Duffy-negative blood types are unique to the African-American community. ... most common blood type and because type O negative blood, in particular, is the universal type needed for ...

  7. Blood Pressure Test

    Science.gov (United States)

    ... an online personal health record or blood pressure tracker, for example. This gives you the option of ... lower your blood pressure. Exercise regularly. Regular physical activity can help lower your blood pressure and keep ...

  8. Blood Clotting and Pregnancy

    Medline Plus

    Full Text Available ... First Edition Abstracts Blood Advances A peer-reviewed, online only, open access journal with a unique focus ... help: Results of Clinical Studies Published in Blood Search Blood , the official journal of ASH, for the ...

  9. Symptoms of Blood Disorders

    Science.gov (United States)

    ... leg (causing most often swelling, redness, and/or warmth of the leg or shortness of breath) Petechiae ( ... Disorders Symptoms of Blood Disorders Medical History and Physical Examination for Blood Disorders Laboratory Tests for Blood ...

  10. Low Blood Pressure (Hypotension)

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    ... and rises sharply on waking. Blood pressure: How low can you go? What's considered low blood pressure ... even life-threatening disorders. Conditions that can cause low blood pressure Some medical conditions can cause low ...

  11. Red blood cell production

    Science.gov (United States)

    ... to one part of the body or another. Red blood cells are an important element of blood. Their job ... is carried to and eliminated by the lungs. Red blood cells are formed in the red bone marrow of ...

  12. Money for Blood and Markets for Blood.

    Science.gov (United States)

    Derpmann, Simon; Quante, Michael

    2015-12-01

    Ontario's Bill 178 proposing a Voluntary Blood Donations Act declares the offer or acceptance of payment for the donation of blood a legal offence and makes it subject to penalty. The bill reinvigorates a fundamental debate about the ethical problems associated with the payment of money for blood. Scarcity of blood donors is a recurring problem in most health systems, and monetary remuneration of the willingness to donate blood is regularly discussed--and sometimes practiced--as a means to overcome scarcity in blood. However, making blood an object of economic exchange has long aroused ethical concerns that often refer to the specific meaning of blood. From the perspective of a modern understanding of money as a metric of economic value, the exchange of money for blood--shed or given--is seen as ethically troubling, because it appears to imply a commensurability of the value of human life and economic wealth. In this paper, we begin with a general taxonomy of the types of arguments that speak in favour or against compensating donors for giving blood. We then describe the context in which the discussion about payment for blood arises, and of the specific aims and concerns that are brought forward in this context. This is used to reconstruct the normative background that supports the rejection of payment for blood as it is envisaged in Bill 178 and the aims of the proposal. We then argue that while a payment indeed changes the nature of a blood donation in an ethically considerable way, we do not believe that decisive arguments against the monetary remuneration of blood donations can be substantiated, at least not independently of assuming specific societal circumstances. Thus it may be possible to establish a stable and safe blood supply through just gratification while at the same time taking strong provisions against social disconnection, injustice, exploitation or heteronomy.

  13. 病毒核酸检测在献血者保留中的应用%The application of nucleic acid testing in keeping blood donor

    Institute of Scientific and Technical Information of China (English)

    李新建

    2013-01-01

    Objective To find out the situation of Hepatitis B virus and Hepatitis C virus in Anyang city by analyzing false positive on ELISA method,and to assess blood samples of voluntary blood donors using detecting nucleic acid of HBV and HCV.Methods ELISA makes blood samples of 50 267 voluntary blood donors,and Real Time PCR tests positive specimens on HBsAg and antibody to HCV,and then blood donors whose results were single positive ELISA and negative NAT would be tracked after 6 months.Results The unmatch rate between two ELISA products in HBV and HCV tests was 46.7%.There were 249 specimens which showed positive in either of two ELISA tests but negative in PCR,and 103 samples that perform positive data in both ELISA products but negative PCR results.The x2 was 114.154 (P<0.01) between single and both positive HBV results,and this statistical item was 61.109 (P<0.01) in HCV comparison.83 of 91 tracked men had ELISA (-) NAT (-) results.Conclusion Real Time PCR could distinguish effectively false positive ELISA results of voluntary blood donors.We should apply Real Time PCR to our volunteers for keeping current blood donors and improving regular ones.%目的 了解安阳地区献血人群中,乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)用酶联免疫吸附法(ELASL)检测的假阳性情况,对无偿献血人群的血标本进行HBV和HCV核酸检测(NAT)的可行性评估.方法 对无偿献血者血液样本50 267份开展ELISA检测,对乙型肝炎病毒表面抗原(HBsAg)和抗丙型肝炎病毒抗体(抗-HCV)阳性标本开展实时荧光定量PCR检测,并对ELISA单试剂(+)NAT(-)献血者6个月后进行追踪检测.结果 两个项目两种ELISA试剂检测结果不相符率达46.7%,ELISA检测方法单试剂阳性者PCR呈阴性者249份,EIISA检测方法双试剂阳性者PCR呈阴性者103份.HBV项目单试剂阳性组与双试剂阳性组比较,差异有统计学意义(x2 =114.154,P<0.01);HCV项目单试剂阳性组与双试剂阳性组比

  14. Application of nucleic acid test in blood donor screening%核酸血筛在献血者常规筛查中的应用研究

    Institute of Scientific and Technical Information of China (English)

    曾劲峰; 许晓绚; 李活; 叶贤林; 陈华; 曹玉峰

    2011-01-01

    Objective To evaluate the necessity and feasibility of nucleic acid test in a large-scale screening of infectious indices of donated blood. Methods Non-reactive samples after ELiSA tests for HBsAg,anti-HBV,anti-HCV,and an-ti-TP were tested with HBV/HCV/HIV nucleic acid test reagent to screen for ELISA non-reactive,nucleic acid test positive samples. NAT-positive samples were followed up further to confirm the serological conversion,or confirmed by imported nucleic acid test reagents. Results With automated detection algorithm of 8-sample minipool, one-time deconvolution of positive pool,out of 55 499 ELiSA negative blood samples, 11 HBV DNA positive,! HCV RNA positive samples were yielded,among which 3 were further confirmed as window period HBV infections by follow-up serological testing,and 1 as window period HCV infection by Roche reagent. The remaining eight cases of HBV DNA-positive samples were confirmed by testing anti-HBsAg,HBeAg,anti-HBeAg,and anTi-HBcAg as occult or other types of HBV infections that were missed by current ELJSA blood screening. Conclusion Nucleic acid blood screening can significantly improve blood safety,especially raising the awareness of more complex hepatitis B virus infections in specimens with low titer. Automated nucleic acid blood screening system can provide sensitive and accurate test results,and are easy to operate,efficient,which fulfill the requirements for daily blood screening.%目的 评估核酸检测在大规模临床用血输血传染性指标筛查中的必要性和实用性.方法 ELISA法检测献血者HBsAg,抗-HBV和抗-HCV,以及抗-TP,非反应性标本再用国产HBV/HCV/HIV核酸检测试剂进行检测,从中筛查出ELISA检测非反应性,核酸检测阳性的标本.进一步对NAT阳性标本跟踪采样检测以确认血清学转化,或用进口核酸试剂复核检测确认核酸阳性.结果 使用8份混样、阳性标本汇集池一次拆分的自动化检测模式,55 499人份ELISA检测合

  15. Natural rates of teacher approval and disapproval in the classroom.

    Science.gov (United States)

    White, M A

    1975-01-01

    Sixteen classroom observational studies were conducted to determine natural rates of teacher verbal approval and disapproval in the classroom. Rates of teacher verbal approval and disapproval were measured by the Teacher Approval and Disapproval Observation Record (TAD) over Grades 1 through 12. Teacher verbal approval rates dropped over grade, with a marked drop after second grade. In every grade after second, the rate of teacher verbal disapproval exceeded the rate of teacher verbal approval. These rates are interpreted in terms of reinforcement theory.

  16. Determination of Δ9-tetrahydrocannabinolic acid A (Δ9-THCA-A) in whole blood and plasma by LC-MS/MS and application in authentic samples from drivers suspected of driving under the influence of cannabis.

    Science.gov (United States)

    Raikos, Nikolaos; Schmid, Helene; Nussbaumer, Susanne; Ambach, Lars; Lanz, Stephan; Längin, Andreas; König, Stefan; Roth, Nadine; Auwärter, Volker; Weinmann, Wolfgang

    2014-10-01

    Delta-9-tetrahydrocannabinolic acid A (THCA-A) is the biosynthetic precursor of delta-9-tetrahydrocannabinol (THC) in cannabis plants, and has no psychotropic effects. THCA-A can be detected in blood and urine, and several metabolites have been identified. THCA-A was also shown to be incorporated in hair by side stream smoke to a minor extent, but incorporation via blood stream or sweat seems unlikely. The detection of THCA-A in biological fluids may serve as a marker for differentiating between the intake of prescribed THC medication - containing only pure THC - and cannabis products containing THC besides THC-acid A and other cannabinoids. However, the knowledge about its usefulness in forensic cases is very limited. The aim of the present work was the development of a reliable method for THCA-A determination in human blood or plasma using LC-MS/MS and application to cases of driving under the influence of drugs. Fifty eight (58) authentic whole blood and the respective plasma samples were collected from drivers suspected of driving under the influence of cannabis from the region of Bern (Switzerland). Samples were first tested for THC, 11-OH-THC and THC-COOH, and then additionally for THCA-A. For this purpose, the existing LC-MS/MS method was modified and validated, and found to be selective and linear over a range of 1.0 to 200ng/mL (the correlation coefficients were above 0.9980 in all validation runs). Limit of detection (LOD) and limit of quantification (LOQ) were 0.3ng/mL and 1.0ng/mL respectively. Intra- and inter-assay accuracy were equal or better than 90% and intra- and inter-assay precision were equal or better than 11.1%. The mean extraction efficiencies were satisfactory being equal or higher than 85.4%. THCA-A was stable in whole blood samples after 3 freeze/thaw cycles and storage at 4°C for 7 days. Re-injection (autosampler) stability was also satisfactory. THC was present in all blood samples with levels ranging from 0.7 to 51ng/mL. THCA

  17. The clinical application of washing type of autologous blood doping in the emergency rescue%洗涤式自体血回输在急诊手术抢救中的临床应用

    Institute of Scientific and Technical Information of China (English)

    陈军民; 陈蕾蕾; 张建华

    2015-01-01

    Objective To explore the clinical application of washing type of autologous blood doping in the emergency rescue,and put forward relevant nursing measures.Methods Selected 60 cases of patients who come to our hospital for emergency rescue at the time from May 2011 to July 2014,recover the blood using blood recycling machine and returning to the patients after processing,and detection of blood routine,blood biochemistry,blood coagulation function,etc.Using SPSS 17.0 statistical software for statistical analysis of relevant data was conducted.Results The amount of blood transfusion of the patients been selected were 500 to 2000 ml,the average of them was 952 ml;all of them had no blood transfusion reaction,and had a good blood doping,no allergy and complications infection;the indicator of routine blood after 24 hours of them had a significantly improvement compared with before the surgery,the difference between different group had statistical significance(P < 0.05);but the indicators of blood biochemistry,blood coagulation function and electrolytes of them had no change compared with before the surgery,which had no statistical significance between them(P > 0.05).Conclusion There had a great clinical effect through using washing type of autologous blood doping in the emergency rescue,which can save blood source effectively,reduce the amount of allogeneic blood transfusion,and also can reduce the occurrence of complications.%目的 探讨洗涤式自体血回输在急诊手术抢救中的临床应用,并提出相关护理措施.方法 选取2011年5月-2014年7月东阳市人民医院收治的进行急诊手术抢救的患者60例作为研究对象,在进行手术时采用自体血液回收机将术中血液回收,经处理后形成浓缩红细胞回输至患者,观察并记录术中患者出血量与自体血回输量,同时对患者血常规、血液生化、凝血功能等情况进行检测,并观察患者有无输血反应、过敏、溶血及感

  18. To Observe the Application of Good Communication Skills for Being Collected Blood in Outpatient Department%良好沟通技巧在门诊采血患者中的应用观察

    Institute of Scientific and Technical Information of China (English)

    李淑清

    2014-01-01

    目的:探讨良好沟通技巧在门诊采血患者中的应用效果。方法选择门诊收治的200例采血患者,随机分为对照组和实验组各100例,两组均接受常规护理,实验组在此基础上应用良好沟通技巧,对比分析两组护理效果。结果实验组患者及其家属护理满意度、紧张、恐惧情绪发生率、皮下淤血发生率等均明显优于对照组(P<0.05)。结论在门诊采血患者临床护理过程中应用良好沟通技巧,有助于患者护理满意度和穿刺成功率的提高。%ObjectiveTo explore the application effect of good communication skills in outpatient blood in patients with.Methods 200 cases of blood of patients in our hospital, were randomly divided into control group and experimental group with 100 cases in each group, the two groups received routine nursing, with good communication skills in experimental group on the basis of this, to compare and analyze the nursing effect of the two groups.Results Nursing patients in experimental group and family satisfaction, tension, fear,the incidence of subcutaneous congestion occurrence rate was significantly better than the control group(P<0.05).Conclusion The application of good communication skils in clinical nursing process of blood colection of patients, help nursing satisfaction of patients and improve the success rate of puncture.

  19. Novel application of pre-operative vertebral body embolization to reduce intraoperative blood loss during a three-column spinal osteotomy for non-oncologic spinal deformity.

    Science.gov (United States)

    Tuchman, Alexander; Mehta, Vivek A; Mack, William J; Acosta, Frank L

    2015-04-01

    Three column osteotomies (3CO) of the lumbar spine are powerful corrective procedures used in the treatment of kyphoscoliosis. Their efficacy comes at the cost of high reported complication rates, notably significant estimated blood loss (EBL). Previously reported techniques to reduce EBL have had modest efficacy. Here we describe a potential technique to decrease EBL during pedicle subtraction osteotomy (PSO) of the lumbar spine by means of pre-operative vertebral body embolization - a technique traditionally used to reduce blood loss prior to spinal column tumor resection. We present a 62-year-old man with iatrogenic kyphoscoliosis who underwent staged deformity correction. Stage 1 involved thoracolumbar instrumentation followed by transarterial embolization of the L4 vertebral body through bilateral segmental arteries. A combination of polyvinyl alcohol particles and Gelfoam (Pfizer, New York, NY, USA) were used. Following embolization there was decreased angiographic blood flow to the small vessels of the L4 vertebral body, while the segmental arteries remained patent. Stage 2 consisted of an L4 PSO and fusion. The EBL during the PSO procedure was 1L, which compared favorably to that during previous PSO at this institution as well as to quantities reported in previous literature. There have been no short term (5 month follow-up) complications attributable to the vertebral body embolization or surgical procedure. Although further investigation into this technique is required to better characterize its safety and efficacy in reducing EBL during 3CO, we believe this patient illustrates the potential utility of pre-operative vertebral embolization in the setting of non-oncologic deformity correction surgery.

  20. Comparison of the Staphylococcus QuickFISH BC test with the tube coagulase test performed on positive blood cultures for evaluation and application in a clinical routine setting.

    Science.gov (United States)

    Carretto, E; Bardaro, M; Russello, G; Mirra, M; Zuelli, C; Barbarini, D

    2013-01-01

    Many studies demonstrate that delayed proper therapy in bloodstream infections caused by Staphylococcus aureus increases the mortality rate, emphasizing the need to shorten the turnaround time for positive blood cultures. Different techniques are currently available, from phenotypic methods to more complex tests such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF), real-time PCR (RT-PCR), and fluorescence in situ hybridization using peptide nucleic acid probes (PNA FISH). This study evaluated the performance of the Staphylococcus QuickFISH BC test (QFT), a novel FISH methodology, compared with the direct tube coagulase test (DTCT) on blood cultures exhibiting Gram-positive cocci in clusters. A total of 173 blood cultures collected from 128 different patients were analyzed using the DTCT, evaluated after both 4 and 24 h, and the QFT. A total of 179 isolates were identified using the Vitek2 system. Thirty-five out of 35 Staphylococcus aureus were correctly identified by the QFT (sensitivity = 100%), with a specificity of 100% (no green fluorescence was detected for strains different from S. aureus). The DTCT was positive after 4 h for 28 out of the 35 samples (sensitivity = 80%) and after 24 h for 31 out of the 35 samples (sensitivity = 88.57%). Among the remaining 144 isolates, one was then identified as Corynebacterium striatum and two as Micrococcus luteus. QFT identified 139 out of the 141 coagulase-negative staphylococci (CoNS) (sensitivity = 98.58%), showing again a specificity of 100% (no fluorescent red signals were detected for strains different from CoNS). We also discuss also the implementation process of this methodology in our setting, with particular emphasis on the workflow and the cost-effectiveness.