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Sample records for antigen cea levels

  1. Carcinoembryonic antigen (CEA) level, CEA ratio, and treatment outcome of rectal cancer patients receiving pre-operative chemoradiation and surgery

    International Nuclear Information System (INIS)

    To investigate serum carcinoembryonic antigen (CEA) as a prognostic factor for rectal cancer patients receiving pre-operative chemoradiotherapy (CRT). Between 2000 and 2009, 138 patients with advanced rectal cancer receiving CRT before surgery at our hospital were retrospectively classified into 3 groups: pre-CRT CEA <6 ng/ml (group L; n = 87); pre-CRT CEA ≥ 6 ng/ml and post-CRT CEA <6 ng/ml (group H-L; n = 32); and both pre- and post-CRT CEA ≥ 6 ng/ml (group H-H; n = 19). CEA ratio (defined as post-CRT CEA divided by pre-CRT CEA), post-CRT CEA level and other factors were reviewed for prediction of pathologic complete response (pCR). Five-year disease-free survival (DFS) was better in groups L (69.0%) and H-L (74.5%) than in group H-H (44.9%) (p = 0.024). Pathologic complete response was observed in 19.5%, 21.9% and 5.3% of groups L, H-L and H-H respectively (p = 0.281). Multivariate analysis showed that ypN stage and pCR were independent prognostic factors for DFS and that post-CRT CEA level was independently predictive of pCR. As a whole, post-CRT CEA <2.61 ng/ml predicted pCR (sensitivity 76.0%; specificity 58.4%). For those with pre-CRT CEA ≥6 ng/ml, post-CRT CEA and CEA ratio both predicted pCR (sensitivity 87.5%, specificity 76.7%). In patients with pre-CRT serum CEA ≥6 ng/ml, those with “normalized” CEA levels after CRT may have similar DFS to those with “normal” (<6 ng/ml) pre-CRT values. Post-CRT CEA level is a predictor for pCR, especially in those with pre-CRT CEA ≥6 ng/ml

  2. Elevated Level of Serum Carcinoembryonic Antigen (CEA) and Search for a Malignancy: A Case Report.

    Science.gov (United States)

    Asad-Ur-Rahman, Fnu; Saif, Muhammad W

    2016-01-01

    Carcinoembryonic antigen (CEA) has been shown to be associated with tumor burden in patients with colorectal cancer. However, it is also elevated to a significant degree in a number of other malignant and non-malignant conditions. We report a case of reversible CEA elevation in a patient using lithium for bipolar disorder. A 58-year-old female with a longstanding smoking history and a past medical history of chronic obstructive pulmonary disease (COPD), bipolar illness, hypothyroidism, and obesity was found to have an elevated CEA level of 11.2 ng/ml (normal level studies to evaluate for colorectal, breast, ovarian, and lung cancer; however, it did not reveal any evidence of malignancy. Upon review of her medications, she reported that she had recently started lithium for her bipolar illness. We followed up her CEA level while her dose of lithium was reduced from 450 to 300 mg per day. Her CEA level decreased from 25 mg/dl to 6.1 mg/dl and remained stable over the course of the next eight months. Our case is the first case report that identifies lithium as a potential cause of reversible CEA elevation. The underlying mechanism is yet to be elucidated, but it underscores the importance of investigating the medications as part of the workup. PMID:27446768

  3. Hookah smoking and cancer: carcinoembryonic antigen (CEA) levels in exclusive/ever hookah smokers

    OpenAIRE

    Chaouachi Kamal; Sajid Khan; Mahmood Rubaida

    2008-01-01

    Abstract Background We have recently published some work on CEA levels in hookah (also called narghile, shisha elsewhere) and cigarette smokers. Hookah smokers had higher levels of CEA than non-smokers although mean levels were low compared to cigarette smokers. However some of them were also users of other tobacco products (cigarettes, bidis, etc.). Objectives To find serum CEA levels in ever/exclusive hookah smokers, i.e. those who smoked only hookah (no cigarettes, bidis, etc.), prepared b...

  4. Protein expression levels of carcinoembryonic antigen (CEA) in Danish ovarian cancer patients: from the Danish 'MALOVA'ovarian cancer study

    DEFF Research Database (Denmark)

    Hogdall, E.V.; Christensen, L.; Blaakaer, J.; Jarle, Christensen,I; Gayther, S.; Jacobs, I.J.; Hogdall, C.K.; Kjær, Susanne Krüger

    2008-01-01

    from 189 women diagnosed with low malignant potential ovarian tumours (LMP, borderline ovarian tumours) and 571 women diagnosed with ovarian cancer (OC). RESULTS: Using 30% as the cut-off level for CEA over-expression, 18% of LMPs and 4% of OCs were positive. A higher proportion of mucinous tumours......AIMS: To determine the variation in expression of carcinoembryonic antigen (CEA) in 760 epithelial ovarian tumours from Denmark, and to correlate expression with clinicopathological parameters and prognosis for the disease. METHODS: Using tissue arrays (TA), we analysed CEA expression in tissues...

  5. Hookah smoking and cancer: carcinoembryonic antigen (CEA levels in exclusive/ever hookah smokers

    Directory of Open Access Journals (Sweden)

    Chaouachi Kamal

    2008-05-01

    Full Text Available Abstract Background We have recently published some work on CEA levels in hookah (also called narghile, shisha elsewhere and cigarette smokers. Hookah smokers had higher levels of CEA than non-smokers although mean levels were low compared to cigarette smokers. However some of them were also users of other tobacco products (cigarettes, bidis, etc.. Objectives To find serum CEA levels in ever/exclusive hookah smokers, i.e. those who smoked only hookah (no cigarettes, bidis, etc., prepared between 1 and 4 times a day with a quantity of up to 120 g of a tobacco-molasses mixture each (i.e. the tobacco weight equivalent of up to 60 cigarettes of 1 g each and consumed in 1 to 8 sessions. Methods Enhanced chemiluminescent immunometric technique was applied to measure CEA levels in serum samples from 59 exclusive male smokers with age ranging from 20–80 years (mean = 58.8 ± 14.7 years and 8–65 years of smoking (mean = 37.7 ± 16.8. 36 non-smokers served as controls. Subjects were divided into 3 groups according to the number of preparations; the number of sessions and the total daily smoking time: Light (1; 1; ≤ 20 minutes; Medium (1–3; 1–3; >20 min to ≤ 2 hrs and Heavy smokers (2–4; 3–8; >2 hrs to ≤ 6 hrs. Because of the nature of distribution of CEA levels among our individuals, Wilcoxon's rank sum two-sample test was applied to compare the variables. Results The overall CEA levels in exclusive hookah smokers (mean: 3.58 ± 2.61 ng/ml; n = 59 were not significantly different (p ≤ 0.0937 from the levels in non-smokers (2.35 ± 0.71 ng/ml. Mean levels in light, medium and heavy smokers were: 1.06 ± 0.492 ng/ml (n = 5; 2.52 ± 1.15 ng/ml (n = 28 and 5.11 ± 3.08 ng/ml (n = 26 respectively. The levels in medium smokers and non-smokers were also not significantly different (p ≤ 0.9138. In heavy smokers, the CEA levels were significantly higher than in non-smokers (p ≤ 0.0001567. Conclusion Overall CEA levels in exclusive hookah

  6. Carcinoembryonic antigen (CEA) dynamics in stomach cancer patients receiving cryotherapy

    International Nuclear Information System (INIS)

    Radioimmunologic assays of blood serum carcinoembryonic antigen (CEA) level were conducted at major stages of treatment of gastric cancer by subtotal stomach resection and gastrectomy with preliminary cryotreatment and thawing of tumor. A short-term rise in CEA level occurred in 53.9 % of cases 3-4 days after combined therapy. A decrease in CEA concentration at discharge from hospital as compared with preoperative level and that registered 3-4 days after operation was observed in 50 and 75 % of cases of combined therapy, respectively, and 47.5 and 37.5 % of controls (surgery without cryotreatment). There was nocorrelation between cryotreatment and changes in CEA level in gastric ulcer patients

  7. Reagents for radioimmunological determination of carcinoembryonic antigen (CEA)

    International Nuclear Information System (INIS)

    The work was undertaken to prepare the reagents for carcinoembryonic antigen (CEA) radioimmunoassay with double antibody method. The CEA standard of high immunoreactivity was prepared and purified. The purified CEA was used for immunozation of goats. The goat anti - CEA sera were received. IgG fraction from normal goat serum was purified and used for the production of horse anti-goat IgG serum which was then used in the radioimmunoassay of CEA. The labelling of CEA with iodine-125 has been carried out be means of the enzymatic method.(Z.R.)

  8. Carcinoembryonic antigen (CEA) as tumor marker in lung cancer

    DEFF Research Database (Denmark)

    Knudsen, Mie Grunnet; Sorensen, J B

    2012-01-01

    The use of CEA as a prognostic and predictive marker in patients with lung cancer is widely debated. The aim of this review was to evaluate the results from studies made on this subject. Using the search words "CEA", "tumor markers in lung cancer", "prognostic significance", "diagnostic...... significance" and "predictive significance", a search was carried out on PubMed. Exclusion criteria was articles never published in English, articles before 1981 and articles evaluating tumor markers in lung cancer not involving CEA. Initially 217 articles were found, and 34 were left after selecting those...... relevant for the present study. Four of these included both Non-Small Cell Lung Cancer (NSCLC) and Small Cell Lung Cancer (SCLC) patients, and 31 dealt solely with NSCLC patients. Regarding SCLC no studies showed that serum level of CEA was a prognostic marker for overall survival (OS). The use of CEA...

  9. Blood CEA levels for detecting recurrent colorectal cancer: A Diagnostic Test Accuracy Review.

    OpenAIRE

    Nicholson, BD; Shinkins, B.; Pathiraja, I; Roberts, NW; James, T; Mallett, S.; Perera, R; Primrose, JN; Mant, D

    2015-01-01

    Background Testing for carcino-embryonic antigen (CEA) in the blood is a recommended part of follow-up to detect recurrence of colorectal cancer following primary curative treatment. There is substantial clinical variation in the cut-off level applied to trigger further investigation. Objectives To determine the diagnostic performance of different blood CEA levels in identifying people with colorectal cancer recurrence in order to inform clinical practice. Search methods W...

  10. CHARACTERIZATION OF MONOCLONAL ANTIBODY CL58 AGAINST CARCINOEMBRYONIC ANTIGEN (CEA) AND STUDY OF ITS BIODISTRIBUTION

    Institute of Scientific and Technical Information of China (English)

    李振甫; 杨志; 张宏; 顾晋

    2002-01-01

    Objective: To study the preparation and characterization of monoclonal antibody (McAb) against carcinoembryonic antigen (CEA). Methods: CEA antigen was extracted from metastasized liver of patients with colorectal cancer and used for the preparation of McAb against CEA by hybridoma technique. Immunoreactivity of McAb to CEA antigen was evaluated using ELISA. Mouse ascites was purified by two steps, high performance liquid chromatography (HPLC) using protein A and high performance hydroxylapatite (HPHT). Normal adult tissues and tumor specimen were used for immunohistochemical evaluation of the McAb. Isotope 99mTc labeled CEA McAb was used for biodistribution in tumor-bearing mouse. Results: Purified CEA antigen was a glycoprotein of 180 kD. Anti-CEA McAb affinity constant was 7.4x109/M. The McAb showed positive staining in 54-88% of colorectal cancer, gastric cancer and lung cancer, while negative for normal tissues. 24 hours after injection of 99mTc labeled McAb, tumor ID%/g was higher than 15% and tumor/blood, tumor/kidney and tumor/liver were 1.82, 1.51 and 2.92 respectively. T/NT ratios of other viscera were over 3.0. Conclusion: Purified CEA antigen had very good immunogenicity. The anti-CEA McAb was highly specific. 99mTc labeled McAb was stabled both in vivo and in vitro. In vivo distribution result was satisfactory. McAb CL58 may be useful for RII and RIGS.

  11. Prognostic Effect of Pretreatment Serum Carcinoembryonic Antigen Level

    OpenAIRE

    Kim, Chang Hyun; Lee, Soo Young; Kim, Hyeong Rok; Kim, Young Jin

    2015-01-01

    Abstract Many studies have reported the prognostic value of pretreatment serum carcinoembryonic antigen (pre-CEA) levels on colorectal cancer outcomes. However, controversy remains concerning the significance of pre-CEA levels in patients with rectal cancer treated with neoadjuvant chemoradiotherapy (CRT). Our aim in this study was to investigate the prognostic role of the pre-CEA level in patients with locally advanced rectal cancer undergoing neoadjuvant CRT followed by total mesorectal exc...

  12. Characterization of gastric adenocarcinoma cell lines established from CEA424/SV40 T antigen-transgenic mice with or without a human CEA transgene

    International Nuclear Information System (INIS)

    Gastric carcinoma is one of the most frequent cancers worldwide. Patients with gastric cancer at an advanced disease stage have a poor prognosis, due to the limited efficacy of available therapies. Therefore, the development of new therapies, like immunotherapy for the treatment of gastric cancer is of utmost importance. Since the usability of existing preclinical models for the evaluation of immunotherapies for gastric adenocarcinomas is limited, the goal of the present study was to establish murine in vivo models which allow the stepwise improvement of immunotherapies for gastric cancer. Since no murine gastric adenocarcinoma cell lines are available we established four cell lines (424GC, mGC3, mGC5, mGC8) from spontaneously developing tumors of CEA424/SV40 T antigen (CEA424/Tag) mice and three cell lines derived from double-transgenic offsprings of CEA424/Tag mice mated with human carcinoembryonic antigen (CEA)-transgenic (CEA424/Tag-CEA) mice (mGC2CEA, mGC4CEA, mGC11CEA). CEA424/Tag is a transgenic C57BL/6 mouse strain harboring the Tag under the control of a -424/-8 bp CEA gene promoter which leads to the development of invasive adenocarcinoma in the glandular stomach. Tumor cell lines established from CEA424/Tag-CEA mice express the well defined tumor antigen CEA under the control of its natural regulatory elements. The epithelial origin of the tumor cells was proven by morphological criteria including the presence of mucin within the cells and the expression of the cell adhesion molecules EpCAM and CEACAM1. All cell lines consistently express the transgenes CEA and/or Tag and MHC class I molecules leading to their susceptibility to lysis by Tag-specific CTL in vitro. Despite the presentation of CTL-epitopes derived from the transgene products the tumor cell lines were tumorigenic when grafted into C57BL/6, CEA424/Tag or CEA424/Tag-CEA-transgenic hosts and no significant differences in tumor take and tumor growth were observed in the different hosts. Although

  13. Metastatic prostate cancer with elevated serum levels of CEA and CA19-9

    Directory of Open Access Journals (Sweden)

    Guang-Dar Juang

    2014-03-01

    Full Text Available Prostate-specific antigen (PSA is well known as a specific tumor marker for prostate cancer, but carcinoembryonic antigen (CEA- and carbohydrate antigen 19-9 (CA19-9-elevating adenocarcinomas originating in the prostate gland are rare. We report a case of metastatic adenocarcinoma of the prostate gland with a high serum level of CEA and CA19-9 in a 78-year-old man in whom prostate cancer (T3N1M1 had been diagnosed 2 years ago and who was treated with androgen deprivation therapy. He visited the emergency department because of a loss of appetite and abdominal pain. The serum CEA and CA19-9 levels were increased to 218.9 ng/mL (normal, <5 ng/mL and 212 ng/mL (normal, <27 ng/mL, respectively. The serum PSA level was slightly elevated (4.41 ng/mL. Computed tomography demonstrated multiple liver metastases, para-aortic lymph node enlargement, and lung metastases. A liver biopsy was performed and the specimen showed high-grade adenocarcinoma with focal positive staining for PSA. Despite chemotherapy with docetaxel, the patient died 3 months after treatment. Based on this case and a review of the literature, an aggressive variant of prostatic carcinoma with a high serum level of CEA and CA19-9 and a low PSA level was shown to progress rapidly with a poor prognosis.

  14. Localization of 131Ilabeled goat and primate anti-carcinoembryonic antigen(CEA) antibodies in patients with cancer

    International Nuclear Information System (INIS)

    Thirty patients with anti-carcinoembryonic antigen (CEA)-producing cancers of the colon, breast, or thyroid were intected with 1 to 2 mCi of Iodine-131 (131I)-labeled, affinity-purified, goat or baboon anti-CEA antibodies. Images were obtained daily for four days. Computerized background subtraction using technetium 99m (99mTC)-labeled compounds was used. Images obtained with and without background subtraction were correlated with other evidence of disease. Activity levels in plasma, urine, and thyroid gland were monitored. Significant deiodination of antibody occurred within the first 24 hours. The mean plasma half-disappearance-time of baboon antibody was significantly longer than the mean half-disappearance-time of goat antibody. With exogenous blockade, total thyroid uptake was less than 0.1% of the injected dose. Without background subtraction, scintigraphic localization of known tumor was possible in one of two patients with colon carcinoma, in three of 20 patients with breast cancer, and in one of five patients with medullary carcinoma of the thyroid. With background subtraction, potential false-positive results could be generated for every patients, depending on the normalization site chosen and the degree of subtraction used. In contrast to results of previous reports, CEA-producing tumor was found to be infrequently localized using highly purified goat or primate radiolabeled anti-CEA. Furthermore, the subtraction technique described by previous investigators may lead to a high false-positive rate

  15. Changes in soluble CEA and TIMP-1 levels during adjuvant chemotherapy for stage III colon cancer

    DEFF Research Database (Denmark)

    Aldulaymi, Bahir; Christensen, Ib Jarle; Sölétormos, György; Jess, Per; Nielsen, Svend Erik; Brünner, Nils; Nielsen, Hans J.

    2010-01-01

    Tissue inhibitor of metalloproteinases-1 (TIMP-1) has been suggested to be a valuable marker in colorectal cancer (CRC), but the effects of chemotherapy on TIMP-1 levels are unknown. The present study evaluated the effect of chemotherapy on TIMP-1 levels in comparison with carcinoembryonic antige...... (CEA) levels in patients with stage III colon cancer.......Tissue inhibitor of metalloproteinases-1 (TIMP-1) has been suggested to be a valuable marker in colorectal cancer (CRC), but the effects of chemotherapy on TIMP-1 levels are unknown. The present study evaluated the effect of chemotherapy on TIMP-1 levels in comparison with carcinoembryonic antigen...

  16. CEA serum level as early predictive marker of outcome during EGFR-TKI therapy in advanced NSCLC patients.

    Science.gov (United States)

    Facchinetti, Francesco; Aldigeri, Raffaella; Aloe, Rosalia; Bortesi, Beatrice; Ardizzoni, Andrea; Tiseo, Marcello

    2015-08-01

    Considering the role of carcinoembryonic antigen (CEA) serum levels as potential useful predictive marker during chemotherapy treatment, we studied its applicability in advanced non-small cell lung cancer (NSCLC) patients treated with epidermal growth factor receptor (EGFR) tyrosine-kinase inhibitors (TKIs). Our retrospective cohort consists of 79 patients (33 EGFR mutated and 46 EGFR wild type or unknown) affected by advanced NSCLC, for whom CEA serum values at the beginning of TKI therapy and after the first month of treatment were available, regardless of treatment line. Baseline CEA value, percentage of CEA reduction after 1 month, and percentage of patients with ≥20 % CEA decrease after 1 month (CEA response) were correlated with disease control rate (DCR), progression-free (PFS), and overall (OS) survival, according to EGFR mutational status. Median baseline CEA levels were significantly higher in EGFR mutated (40.9 ng/ml; interquartile range (IQR) 8.9-197.6) than in wild-type cases (6.2 ng/ml; IQR 2.8-12.8; p = 0.003). Both percentage reduction in CEA levels (-10.7 vs. +13.4 %) and percentage of cases with CEA response (42 vs. 20 %) were significantly higher in mutated vs. wild-type/unknown patients (p = 0.007 and p = 0.027, respectively). In wild-type/unknown patients, CEA response was significantly correlated with DCR (p = 0.001) and resulted as a significant predictor of PFS both in univariate (p = 0.002) and in multivariate analyses (hazard ratio (HR) 0.27; 95 % confidence interval (CI) 0.11-0.66; p = 0.004); only a trend was found for OS prediction (p = 0.082). In EGFR-mutated group, CEA reduction did not show any correlation either with PFS or OS. CEA response after 1 month of EGFR-TKI therapy could be a useful marker, worthy to further studies, as early predictor of treatment outcome in EGFR wild-type/unknown unselected NSCLC cases for which no molecular predictor is yet available. PMID:25731731

  17. CEA contribution to power plant operation with high burnup level

    International Nuclear Information System (INIS)

    High level burnup in PWR leads to investigate again the choices carried out in the field of fuel management. French CEA has studied the economic importance of reshuffling technique, cycle length, discharge burnup, and non-operation period between two cycles. Power plants operators wish to work with increased length cycles of 18 months instead of 12. That leads to control problems because the core reactivity cannot be controlled with the only soluble boron: moderator temperature coefficient must be negative. With such cycles, it is necessary to use burnable poisons and for economic reasons with a low penalty in end of cycle. CEA has studied the use of Gd2O3 mixed with fuel or with inert element like Al2O3. Parametric studies of specific weights, efficacities relatively to the fuel burnup and the fuel enrichment have been carried out. Particular studies of 1 month cycles with Gd2O3 have shown the possibility to control power distribution with a very low reactivity penalty in EOC. In the same time, in the 100 MW PWR-CAP, control reactivity has been made with large use of gadolinia in parallel with soluble boron for the two first cycles

  18. Expression of a neuroendocrine gene signature in gastric tumor cells from CEA 424-SV40 large T antigen-transgenic mice depends on SV40 large T antigen.

    Directory of Open Access Journals (Sweden)

    Fritz Ihler

    Full Text Available BACKGROUND: A large fraction of murine tumors induced by transgenic expression of SV40 large T antigen (SV40 TAg exhibits a neuroendocrine phenotype. It is unclear whether SV40 TAg induces the neuroendocrine phenotype by preferential transformation of progenitor cells committed to the neuroendocrine lineage or by transcriptional activation of neuroendocrine genes. METHODOLOGY/PRINCIPAL FINDINGS: To address this question we analyzed CEA424-SV40 TAg-transgenic mice that develop spontaneous tumors in the antral stomach region. Immunohistology revealed expression of the neuroendocrine marker chromogranin A in tumor cells. By ELISA an 18-fold higher level of serotonin could be detected in the blood of tumor-bearing mice in comparison to nontransgenic littermates. Transcriptome analyses of antral tumors combined with gene set enrichment analysis showed significant enrichment of genes considered relevant for human neuroendocrine tumor biology. This neuroendocrine gene signature was also expressed in 424GC, a cell line derived from a CEA424-SV40 TAg tumor, indicating that the tumor cells exhibit a similar neuroendocrine phenotype also in vitro. Treatment of 424GC cells with SV40 TAg-specific siRNA downregulated expression of the neuroendocrine gene signature. CONCLUSIONS/SIGNIFICANCE: SV40 TAg thus appears to directly induce a neuroendocrine gene signature in gastric carcinomas of CEA424-SV40 TAg-transgenic mice. This might explain the high incidence of neuroendocrine tumors in other murine SV40 TAg tumor models. Since the oncogenic effect of SV40 TAg is caused by inactivation of the tumor suppressor proteins p53 and RB1 and loss of function of these proteins is commonly observed in human neuroendocrine tumors, a similar mechanism might cause neuroendocrine phenotypes in human tumors.

  19. Preoperative serum levels of CEA and CA 242 in colorectal cancer.

    OpenAIRE

    Carpelan-Holmström, M; Haglund, C.; Kuusela, P.; Järvinen, H; Roberts, P. J.

    1995-01-01

    Preoperative serum levels of CEA and CA 242 were determined in 260 patients with colorectal cancer and in 92 patients with benign colorectal diseases. The overall sensitivity of the CEA test was 43% and of the CA 242 test 39%. The corresponding specificities were 90% and 87% respectively, using 5 ng ml-1 as cut-off level for CEA and 20 U ml-1 for CA 242. The sensitivity of CEA was 26%, 32%, 38% and 77% for Dukes A, B, C and D colorectal cancer, and the sensitivity of CA 242 was 26%, 26%, 40% ...

  20. High Plasma TIMP-1 and Serum CEA Levels during Combination Chemotherapy for Metastatic Colorectal Cancer Are Significantly Associated with Poor Outcome

    DEFF Research Database (Denmark)

    Aldulaymi, Bahir; Byström, Per; Berglund, Ake; Christensen, Ib J; Brünner, Nils; Nielsen, Hans Jørgen; Glimelius, Bengt

    overall survival. Materials and Methods: Eighty-eight patients with mCRC were included. Blood samples were collected before initiation and after 2, 4 and 6 weeks of treatment with an irinotecan-5-fluorouracil combination. Plasma TIMP-1 and serum CEA levels were determined by validated ELISA platforms. The...... associated with poor overall survival; p <0.0001 in all 4 determinations. A similar association between serum CEA and overall survival could only be demonstrated before treatment. Conclusion: Median plasma TIMP-1 or serum CEA levels do not change significantly during the first 6 weeks of chemotherapy for m......Objective: To evaluate whether combination chemotherapy leads to early changes in plasma TIMP-1 and serum carcinoembryonic antigen (CEA) levels in patients with metastatic colorectal cancer (mCRC), and whether such changes relate to subsequent objective response, time to progression (TTP) and...

  1. A combination of preoperative CT findings and postoperative serum CEA levels improves recurrence prediction for stage I lung adenocarcinoma

    International Nuclear Information System (INIS)

    Objectives: To assess the prognostic value of combined evaluation of preoperative CT findings and pre/postoperative serum carcinoembryonic antigen (CEA) levels for pathological stage I lung adenocarcinoma. Methods: This retrospective study included 250 consecutive patients who underwent complete resection for ≤3-cm pathological stage I (T1–2aN0M0) adenocarcinomas (132 men, 118 women; mean age, 67.8 years). Radiologists evaluated following CT findings: maximum tumor diameter, percentage of solid component (%solid), air bronchogram, spiculation, adjacency of bullae or interstitial pneumonia (IP) around the tumor, notch, and pleural indent. These CT findings, pre/postoperative CEA levels, age, gender, and Brinkman index were assessed by Cox proportional hazards model to determine the best prognostic model. Prognostic accuracy was examined using the area under the receiver operating characteristic curve (AUC). Results: Median follow-up period was 73.2 months. In multivariate analysis, high %solid, adjacency of bullae or IP around the tumor, and high postoperative CEA levels comprised the best combination for predicting recurrence (P < 0.05). A combination of these three findings had a greater accuracy in predicting 5-year disease-free survival than did %solid alone (AUC = 0.853 versus 0.792; P = 0.023), with a sensitivity of 85.7% and a specificity of 74.3% at the optimal threshold. The best cut-off values of %solid and postoperative CEA levels for predicting high-risk patients were ≥48% and ≥3.7 ng/mL, respectively. Conclusion: Compared to %solid alone, combined evaluation of %solid, adjacency of bullae or IP change around the tumor, and postoperative CEA levels improves recurrence prediction for stage I lung adenocarcinoma

  2. A combination of preoperative CT findings and postoperative serum CEA levels improves recurrence prediction for stage I lung adenocarcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Yamazaki, Motohiko, E-mail: xackey2001@gmail.com [Department of Radiology, Niigata University Graduate School of Medical and Dental Sciences (Japan); Ishikawa, Hiroyuki [Department of Radiology, Niigata University Graduate School of Medical and Dental Sciences (Japan); Kunii, Ryosuke [Division of Cellular and Molecular Pathology, Niigata University Graduate School of Medical and Dental Sciences (Japan); Tasaki, Akiko; Sato, Suguru; Ikeda, Yohei; Yoshimura, Norihiko [Department of Radiology, Niigata University Graduate School of Medical and Dental Sciences (Japan); Hashimoto, Takehisa; Tsuchida, Masanori [Division of Thoracic and Cardiovascular Surgery, Niigata University Graduate School of Medical and Dental Sciences (Japan); Aoyama, Hidefumi [Department of Radiology, Niigata University Graduate School of Medical and Dental Sciences (Japan)

    2015-01-15

    Objectives: To assess the prognostic value of combined evaluation of preoperative CT findings and pre/postoperative serum carcinoembryonic antigen (CEA) levels for pathological stage I lung adenocarcinoma. Methods: This retrospective study included 250 consecutive patients who underwent complete resection for ≤3-cm pathological stage I (T1–2aN0M0) adenocarcinomas (132 men, 118 women; mean age, 67.8 years). Radiologists evaluated following CT findings: maximum tumor diameter, percentage of solid component (%solid), air bronchogram, spiculation, adjacency of bullae or interstitial pneumonia (IP) around the tumor, notch, and pleural indent. These CT findings, pre/postoperative CEA levels, age, gender, and Brinkman index were assessed by Cox proportional hazards model to determine the best prognostic model. Prognostic accuracy was examined using the area under the receiver operating characteristic curve (AUC). Results: Median follow-up period was 73.2 months. In multivariate analysis, high %solid, adjacency of bullae or IP around the tumor, and high postoperative CEA levels comprised the best combination for predicting recurrence (P < 0.05). A combination of these three findings had a greater accuracy in predicting 5-year disease-free survival than did %solid alone (AUC = 0.853 versus 0.792; P = 0.023), with a sensitivity of 85.7% and a specificity of 74.3% at the optimal threshold. The best cut-off values of %solid and postoperative CEA levels for predicting high-risk patients were ≥48% and ≥3.7 ng/mL, respectively. Conclusion: Compared to %solid alone, combined evaluation of %solid, adjacency of bullae or IP change around the tumor, and postoperative CEA levels improves recurrence prediction for stage I lung adenocarcinoma.

  3. High Plasma TIMP-1 and Serum CEA Levels during Combination Chemotherapy for Metastatic Colorectal Cancer Are Significantly Associated with Poor Outcome

    DEFF Research Database (Denmark)

    Aldulaymi, Bahir; Byström, Per; Berglund, Ake;

    2010-01-01

    Objective: To evaluate whether combination chemotherapy leads to early changes in plasma TIMP-1 and serum carcinoembryonic antigen (CEA) levels in patients with metastatic colorectal cancer (mCRC), and whether such changes relate to subsequent objective response, time to progression (TTP) and...... response. Moreover, high levels of plasma TIMP-1 before treatment and at weeks 2 and 4 were significantly associated with short TTP, while high levels of serum CEA at week 4 were significantly associated with short TTP. Finally, high levels of plasma TIMP-1 before and during treatment were significantly...

  4. Nomograms for Predicting the Prognostic Value of Pre-Therapeutic CA15-3 and CEA Serum Levels in TNBC Patients.

    Science.gov (United States)

    Dai, Danian; Chen, Bo; Tang, Hailin; Wang, Bin; Zhao, Zhiping; Xie, Xiaoming; Wei, Weidong

    2016-01-01

    Previous studies have indicated that carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA15-3) levels are both independent prognostic factors in breast cancer. However, the utility of CEA and CA15-3 levels as conventional cancer biomarkers in patients with triple-negative breast cancer (TNBC) remains controversial. The current study was performed to explore the predictive value of pre-therapeutic serum CEA and CA15-3 levels, and nomograms were developed including these serum cancer biomarkers to improve the prognostic evaluation of TNBC patients. Pre-therapeutic CA15-3 and CEA concentrations were measured in 247 patients with stage I-IV TNBC. Kaplan-Meier analysis showed that TNBC patients with high levels of both CEA and CA15-3 had shorter overall survival (OS) and disease-free survival (DFS) rates than those in the low-level groups (ppersonal forecasts of OS and DFS for patients with TNBC. These novel nomograms may help physicians to select the optimal treatment plans to ensure the best outcomes for TNBC patients. PMID:27561099

  5. Clinical Value of Serum HMGB1 Levels in Early Detection of Recurrent Squamous Cell Carcinoma of Uterine Cervix: Comparison with Serum SCCA, CYFRA21-1, and CEA Levels

    OpenAIRE

    Sheng, Xiugui; Du, Xuelian; Zhang, Xiaoling; Li, Dapeng; Lu, Chunhua; Li, Qinshui; Ma, Zhifang; Song, Quqing; Wang, Cong

    2009-01-01

    Aim To evaluate the clinical value of serum high mobility group box chromosomal protein 1 (HMGB1) levels in making the early diagnosis of recurrent cervical squamous cell carcinomas (CSCC) and compare it with the value of serum squamous cell carcinoma antigen (SCCA), cytokeratin fragment (CYFRA) 21-1, and carcinoembryonic antigen (CEA) levels. Methods Immunohistochemical staining of tissue from 64 patients with recurrent CSCCs, 72 patients with non-recurrent carcinoma,...

  6. Biliary carcinoembryonic antigen levels in diagnosis of occult hepatic metastases from colorectal carcinoma

    Institute of Scientific and Technical Information of China (English)

    Jaques Waisberg; Rog(e)rio T. Palma; Lu(i)s Contim Neto; Lourdes C. Martins; Maur(i)cio S. L. Oliveira; Carlos A. Nagashima; Antonio C. Godoy; Fabio S. Goffi

    2003-01-01

    AIM: To prospectively explore the role of carcinoembryonic antigen (CEA) in gallbladder bile in patients with colorectal carcinoma and the morphological and clinical features of neoplasia and the occurrence of hepatic metastases.METHODS: CEA levels in the gallbladder and peripheral blood were studied in 44 patients with colorectal carcinoma and 10 patients with uncomplicated cholelithiasis. CEA samples were collected from the gallbladder bile and peripheral blood during the operation, immediately before extirpating the colorectal neoplasia or cholecystectomy.Values of up to 5 ng/ml were considered normal for bile and serum CEA.RESULTS: In the 44 patients with colorectal carcinoma who underwent operation with curative intent, the average level of serum CEA was 8.5 ng/ml (range: 0.1 to 111.0 ng/ml) and for bile CEA it was 74.5 ng/ml (range: 0.2 to 571.0ng/ml). In the patients with uncomplicated cholelithiasis who underwent cholecystectomy, the average level of serum CEA was 1.9 ng/ml (range: 1.0 to 3.5 ng/ml) and for bile CEA it was 1.2 ng/ml (range: 0.3 to 2.9 ng/ml).The average duration of follow-up time was 16.5 months (range: 6 to 48 months). Four patients who underwent extirpation of the colorectal carcinoma without evidence of hepatic metastasis and with an average bile CEA value of 213.2 ng/ml presented hepatic metastases between three and seventeen months after removal of the primary colorectal neoplasia. Three of them successfully underwent extirpation of the hepatic lesions.CONCLUSION: High CEA levels in gallbladders of patients undergoing curative operation for colorectal carcinoma may indicate the presence of hepatic metastases. Such patients must be followed up with special attention to the diagnosis of such lesions.

  7. Levels of estrogen, carcinoembryonic antigen and cancer antigen of breast in breast cancer patients

    International Nuclear Information System (INIS)

    This study was conducted during the period from february 2004 to July 2004; with the objective of measuring the levels of estrogen (E2), carcinoembryonic antigen (CEA) and cancer antigen of breast (CA-15.3) so as to facilitate the early diagnosis of breast cancer and determine the involvement of these parameters as risk factors for breast cancer. Ninety blood samples were collected from Sudanese females, divided into two groups; control group and patient groups. The patients group was sixty Sudanese females visiting the Radio Isotope Center, Khartoum (RICK) and they were confirmed as breast cancer patient by histopathology. The levels of the above mentioned parameters were determined by using radioimmunoassay technique. The results showed that, no significant (p=0.05) difference between the levels of the estrogen in patients compared to the control, on the other hand there was non significant (p>0.05) elevation in CEA levels in the patients with breast cancer compared to the control. The level of CA15.3 was significantly (p<0.0001) higher in the breast cancer patients compared to the control.(Author)

  8. Synuclein gamma predicts poor clinical outcome in colon cancer with normal levels of carcinoembryonic antigen

    Directory of Open Access Journals (Sweden)

    Xing Xiaofang

    2010-07-01

    Full Text Available Abstract Background Synuclein gamma (SNCG, initially identified as a breast cancer specific gene, is aberrantly expressed in many different malignant tumors but rarely expressed in matched nonneoplastic adjacent tissues. In this study, we investigated the prognostic potential of SNCG in colon cancer particularly in the patients with normal carcinoembryonic antigen (CEA levels. Methods SNCG levels were assessed immunohistochemically in cancer tissues from 229 colon adenocarcinoma patients with a mean follow-up of 44 months. Correlations between SNCG levels and clinicopathologic features, preoperative serum CEA level, and clinical outcome were analyzed statistically using SPSS. Results SNCG levels in colon adenocarcinoma were closely associated with intravascular embolus and tumor recurrence but independent of preoperative serum CEA levels. SNCG expression was an independent prognostic factor of a shorter disease-free survival (DFS and overall survival (OS (P P = 0.001, P = 0.001, 0.002 for 97 patients with normal preoperative serum CEA level. Conclusions Our results suggest for the first time that SNCG is a new independent predicator for poor prognosis in patients with colon adenocarcinoma, including those with normal CEA levels. Combination of CEA with SNCG improves prognostic evaluation for patients with colon adenocarcinoma.

  9. Value of {sup 18}F-FDG uptake on PET/CT and CEA level to predict epidermal growth factor receptor mutations in pulmonary adenocarcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Kai-Hsiung; Hsu, Hsian-He; Chang, Wei-Chou; Hsu, Yi-Chih; Chang, Tsun-Hou [Tri-Service General Hospital and National Defense Medical Center, Department of Radiology, Taipei 114 (China); Huang, Tsai-Wang; Chang, Hung [Tri-Service General Hospital and National Defense Medical Center, Department of Thoracic Surgery, Taipei (China); Gao, Hong-Wei [Tri-Service General Hospital and National Defense Medical Center, Department of Pathology, Taipei (China); Shen, Daniel H.Y. [Tri-Service General Hospital and National Defense Medical Center, Department of Nuclear medicine, Taipei (China); Chu, Chi-Ming [Institute of Public Health, National Defense Medical Center and University, Section of Health Informatics, Taipei (China); Ho, Ching-Liang [Tri-Service General Hospital and National Defense Medical Center, Division of Hematology-Oncology, Department of Internal Medicine, Taipei (China)

    2014-10-15

    The identification of the mutation status of the epidermal growth factor receptor (EGFR) is important for the optimization of treatment in patients with pulmonary adenocarcinoma. The acquisition of adequate tissues for EGFR mutational analysis is sometimes not feasible, especially in advanced-stage patients. The aim of this study was to predict EGFR mutation status in patients with pulmonary adenocarcinoma based on {sup 18}F-fluorodeoxyglucose (FDG) uptake and imaging features in positron emission tomography/computed tomography (PET/CT), as well as on the serum carcinoembryonic antigen (CEA) level. We retrospectively reviewed 132 pulmonary adenocarcinoma patients who underwent EGFR mutation testing, pretreatment FDG PET/CT and serum CEA analysis. The associations between EGFR mutations and patient characteristics, maximal standard uptake value (SUVmax) of primary tumors, serum CEA level and CT imaging features were analyzed. Receiver-operating characteristic (ROC) curve analysis was performed to quantify the predictive value of these factors. EGFR mutations were identified in 69 patients (52.2 %). Patients with SUVmax ≥6 (p = 0.002) and CEA level ≥5 (p = 0.013) were more likely to have EGFR mutations. The CT characteristics of larger tumors (≥3 cm) (p = 0.023) and tumors with a nonspiculated margin (p = 0.026) were also associated with EGFR mutations. Multivariate analysis showed that higher SUVmax and CEA level, never smoking and a nonspiculated tumor margin were the most significant predictors of EGFR mutation. The combined use of these four criteria yielded a higher area under the ROC curve (0.82), suggesting a good discrimination. The combined evaluation of FDG uptake, CEA level, smoking status and tumor margins may be helpful in predicting EGFR mutation status in patients with pulmonary adenocarcinoma, especially when the tumor sample is inadequate for genetic analysis or genetic testing is not available. Further large-scale prospective studies are

  10. Diagnostic value of combined determination of serum tumor markers (NSE, CA-242, TPA, CEA) levels in patients with lung cancer

    International Nuclear Information System (INIS)

    Objective: To investigate the diagnostic value of combined determination of serum NSE, CA242, tissue polypeptide antigen (TPA) and CEA levels in patients with primary lung cancer. Methods: Serum NSE, CA242, TPA and CEA levels were determined with ELISA in (1) 102 patients with various types of primary lung carcinoma (adenocarcinoma 38, squamous cell carcinoma 32, small cell lung carcinoma 32) (2) 33 patients with open lung T. B. and (3) 30 controls. Results: (1) In patients with lung cancer, serum levels of all the four markers were increased and significantly higher than their respective values in patients with open lung T.B. and controls. (2) Positive rate of combined any two markers were 75% for adenocarcinoma, 50% for squamous cell carcinoma and 65% for small cell lung carcinoma, while false positive rate was only 9% for T.B patients and none for the controls. (3) The most appropriate single marker for each specific type of lung cancer was: NSE for SCLC (sensitivity 72%, specificity 97%, CA242 for adenocarcinoma sensitivity 62%, specificity 90%). Conclusion: Combined determination of these tumor markers would improve the sensitivity and specificity for diagnosis of primary lung carcinoma. (authors)

  11. Clinical evaluation of radioassays for calcitonin, CEA-S, AFP and ferritin as tumor markers

    International Nuclear Information System (INIS)

    Clinical usefulness of the radioassays for serum calcitonin, isometric species of carcinoembryonic antigen (CEA-S), alpha-fetoprotein (AFP) and ferritin as the specific tumor markers was evaluated. The calcitonin assay was found to be extremely useful in detecting the cases of familial medullary carcinoma of the thyroid and in monitoring the patients after surgery. The CEA-S assay was proved more specific in titers and positivity for malignancies, especially GI tract carcinomas than CEA assay. The diagnosis of hepatoma could be performed precisely with a combination of 67Ga scintigraphy and assays of CEA and AFP. The serum ferritin levels in malignancies overlapped widely with those of non-malignancies. However, the estimation of ferritin-iron ratio was thought to be a useful means for screening patients with suspicious lesions. (author)

  12. Combined determination of CEA, Fer, NSE and SCC levels in serum and chest effusion fluid for diagnosis of pulmonary cancer

    International Nuclear Information System (INIS)

    Objective: To study the clinical value of combined determination of CEA, Fer, NSE and SCC levels in serum and chest effusion fluid for dignosis of lung cancer. Methods: These four tumor markers were determined in serum and chest effusion fluid with RIA (NSE and SCC) or chemiluminescence method (CEA and Fer) in 52 patients with lung cancer, 48 patients with benign lung disease and 51 controls. Results: The serum and chest effusion fluid levels of CEA, Fer, NSE and SCC in patients with lung cancer were significantly higher than those in patients with benign lung diseases (all P<0.01) and 51 controls (P<0.001, <0.001, <0. 01 and <0.01 respectively). The positive rate of combined determination of CEA, Fer, NSE and SCC for lung cancer could be above 92.7%. Conclusion: Combined determination of CEA, Fer, NSE and SCC were a useful method for lung cancer diagnosis. (authors)

  13. Anti-CEA monoclonal antibody: technetium-99m labeling and the validation process of a scintigraphic animal model with a non-cellular antigenic implant.

    Science.gov (United States)

    Sapienza, Marcelo Tatit; Marques, Fabio Luiz Navarro; Okamoto, Miriam Roseli Yoshie; Hironaka, Fausto Haruki; Buchpiguel, Carlos Alberto

    2002-07-01

    Animal models are currently used to verify the biodistribution of different radiopharmaceuticals before its clinical application in Nuclear Medicine; however, there may be some limitations. The utilization of labelled anti-tumor monoclonal antibodies (MoAb) in experimental models often requires implant of human antigens (usually a cellular implant), which cannot be achieved in immunocompetent animals. Our purpose was to label an anti-CEA MoAb with technetium-99m (99Tc) and to validate a simplified animal model using a noncellular antigenic implant. MoAb was directly labelled with 99mTc, after reduction with 2-mercaptoethanol. Labeling efficiency was checked by ascending chromatography and immunoreactive fraction was measured in plastic wells sensitized with the antigen. Radiopharmaceutical biodistribution was evaluated by dissection and scintigraphy in 5 mice groups; following the subcutaneous administration of Al(OH)3, CEA adsorbed Al(OH)2 and a control group evaluation. Labeling efficiency was 94+/-3%, which showed to be stable for 24 hr, with immunoreactive fraction above 50%. Invasive biodistribution evaluation showed prolonged blood retention, hepatic and renal uptake. A significant increase in uptake was observed in scintigraphic studies of animals with CEA-adsorbed Al(OH)3 implants compared with the other groups (p<0.05). The non-cellular antigenic implant model simplifies the pre-clinical evaluation of labelled MoAb. PMID:12146705

  14. Fully human IgG and IgM antibodies directed against the carcinoembryonic antigen (CEA) Gold 4 epitope and designed for radioimmunotherapy (RIT) of colorectal cancers

    International Nuclear Information System (INIS)

    Human monoclonal antibodies (MAbs) are needed for colon cancer radioimmunotherapy (RIT) to allow for repeated injections. Carcinoembryonic antigen (CEA) being the reference antigen for immunotargeting of these tumors, we developed human anti-CEA MAbs. XenoMouse®-G2 animals were immunized with CEA. Among all the antibodies produced, two of them, VG-IgG2κ and VG-IgM, were selected for characterization in vitro in comparison with the human-mouse chimeric anti-CEA MAb X4 using flow cytometry, surface plasmon resonance, and binding to radiolabeled soluble CEA and in vivo in human colon carcinoma LS174T bearing nude mice. Flow cytometry analysis demonstrated binding of MAbs on CEA-expressing cells without any binding on NCA-expressing human granulocytes. In a competitive binding assay using five reference MAbs, directed against the five Gold CEA epitopes, VG-IgG2κ and VG-IgM were shown to be directed against the Gold 4 epitope. The affinities of purified VG-IgG2κ and VG-IgM were determined to be 0.19 ± 0.06 × 108 M-1 and 1.30 ± 0.06 × 108 M-1, respectively, as compared with 0.61 ± 0.05 × 108 M-1 for the reference MAb X4. In a soluble phase assay, the binding capacities of VG-IgG2κ and VG-IgM to soluble CEA were clearly lower than that of the control chimeric MAb X4. A human MAb concentration of about 10-7 M was needed to precipitate approximatively 1 ng 125I-rhCEA as compared with 10-9 M for MAb X4, suggesting a preferential binding of the human MAbs to solid phase CEA. In vivo, 24 h post-injection, 125I-VG-IgG2κ demonstrated a high tumor uptake (25.4 ± 7.3%ID/g), close to that of 131I-X4 (21.7 ± 7.2%ID/g). At 72 h post-injection, 125I-VG-IgG2κ was still concentrated in the tumor (28.4 ± 11.0%ID/g) whereas the tumor concentration of 131I-X4 was significantly reduced (12.5 ± 4.8%ID/g). At no time after injection was there any accumulation of the radiolabeled MAbs in normal tissues. A pertinent analysis of VG-IgM biodistribution was not possible in this

  15. Fully human IgG and IgM antibodies directed against the carcinoembryonic antigen (CEA Gold 4 epitope and designed for radioimmunotherapy (RIT of colorectal cancers

    Directory of Open Access Journals (Sweden)

    Pugnière Martine

    2004-10-01

    Full Text Available Abstract Background Human monoclonal antibodies (MAbs are needed for colon cancer radioimmunotherapy (RIT to allow for repeated injections. Carcinoembryonic antigen (CEA being the reference antigen for immunotargeting of these tumors, we developed human anti-CEA MAbs. Methods XenoMouse®-G2 animals were immunized with CEA. Among all the antibodies produced, two of them, VG-IgG2κ and VG-IgM, were selected for characterization in vitro in comparison with the human-mouse chimeric anti-CEA MAb X4 using flow cytometry, surface plasmon resonance, and binding to radiolabeled soluble CEA and in vivo in human colon carcinoma LS174T bearing nude mice. Results Flow cytometry analysis demonstrated binding of MAbs on CEA-expressing cells without any binding on NCA-expressing human granulocytes. In a competitive binding assay using five reference MAbs, directed against the five Gold CEA epitopes, VG-IgG2κ and VG-IgM were shown to be directed against the Gold 4 epitope. The affinities of purified VG-IgG2κ and VG-IgM were determined to be 0.19 ± 0.06 × 108 M-1 and 1.30 ± 0.06 × 108 M-1, respectively, as compared with 0.61 ± 0.05 × 108 M-1 for the reference MAb X4. In a soluble phase assay, the binding capacities of VG-IgG2κ and VG-IgM to soluble CEA were clearly lower than that of the control chimeric MAb X4. A human MAb concentration of about 10-7 M was needed to precipitate approximatively 1 ng 125I-rhCEA as compared with 10-9 M for MAb X4, suggesting a preferential binding of the human MAbs to solid phase CEA. In vivo, 24 h post-injection, 125I-VG-IgG2κ demonstrated a high tumor uptake (25.4 ± 7.3%ID/g, close to that of 131I-X4 (21.7 ± 7.2%ID/g. At 72 h post-injection, 125I-VG-IgG2κ was still concentrated in the tumor (28.4 ± 11.0%ID/g whereas the tumor concentration of 131I-X4 was significantly reduced (12.5 ± 4.8%ID/g. At no time after injection was there any accumulation of the radiolabeled MAbs in normal tissues. A pertinent analysis of

  16. The effect of high level natural ionizing radiation on expression of PSA, CA19-9 and CEA tumor markers in blood serum of inhabitants of Ramsar, Iran

    International Nuclear Information System (INIS)

    Since several high level natural radiation areas (HLNRAs) exist on our planet, considerable attention has been drawn to health issues that may develop as the result of visiting or living in such places. City of Ramsar in Iran is an HNLRA, and is a tourist attraction mainly due to its hot spas. However, the growing awareness over its natural radiation sources has prompted widespread scientific investigation at national level. In this study, using an ELISA method, the level of expression of three tumor markers known as carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and carcino antigen 19-9 (CA19-9) in blood serum of 40 local men of Ramsar (subject group) was investigated and compared to 40 men from the city of Noshahr (control group). Noshahr was previously identified as a normal level natural radiation area (NLNRA) that is some 85 km far from Ramsar. According to statistical analysis, there was a significant difference in the levels of PSA and CA19-9 markers between the two groups (p < 0.001) with those of Ramsar being considerably higher. CEA level did not show any difference. Although some of the volunteers tested positive to the markers, they were in good health as confirmed by the physician. Moreover, the high number of positive markers in Noshahr was considerable. Therefore, future study is needed to further validate this result and to determine the level of positivity to tumor markers in both cities. -- Highlights: • Expression of three tumor markers was examined in 80 volunteers from Ramsar. • There was a significant difference in the levels of PSA and CA19-9 markers. • Some of the volunteers from the control city of Noshahr also tested positive. • Radiation might have caused an adaptive response in people of Ramsar. • Further study is necessary to re-confirm the positivity to tumor marker

  17. CEA (Carcinoembryonic Antigen) Test

    Science.gov (United States)

    ... may also be used as a marker for medullary thyroid cancer and cancers of the rectum, lung , ... ulcerative colitis , rectal polyps , emphysema , and benign breast disease. ^ Back to top Proudly sponsored by ... Learn more ...

  18. The possible role of tumor antigen CA 15-3, CEA and ferritin in malignant and benign disease

    Directory of Open Access Journals (Sweden)

    Nafija Serdarević

    2012-09-01

    Full Text Available Introduction: Serum CA15-3 has been one of the most reliable tumor markers used in monitoring of breast cancer patients. To increase its sensitivity, the combined measurement of other tumor markers (CEA and ferritin with CA15-3 was investigated. The aim of this study was determination of CA 15-3, CEA and ferritin in female patients with breast cancer, lung cancer and mastitisMethods: 300 patients with carcinoma, hospitalized at Department of Gynecologic Oncology and Department for Oncology at the University Clinics Center of Sarajevo and 200 healthy subjects were compared.Results: In patients with breast cancer the mean value of tumor markers were CEA 155.61 ng/mL, CA 15-3 106.38 U/mL and ferritin 197.03 ng/mL. In patients with lung cancer CEA was 58.97 ng/ml, CA 15-3 40.62 U/mL and ferritin 544.16 ng/mL. Patients with mastitis had CEA 5.17 ng/mL, CA 15-3 112.67 U/mL and ferritin 174.92 ng/mL. The control group had values of tumor markers CEA 1.62 ng/mL, CA 15-3 11.72 U/mL and ferritin 85.35 ng/mL. We found good correlation between CA 15-3 and CEA correlation coeffi cient was r = 0.750. There was a low correlation between CA 15-3 and ferritin with correlation coeffi cient r = 0.274.Conclusions: The CA 15-3 and CEA are useful markers in patients with confi rmed diagnosis of breast and lung cancers. The ferritin concentration has not increased in patients with breast cancer but it increased inlung patients. The future study has to make investigations of tumor markers and ferritin in different stage of breast cancer.

  19. CEA Level, Radical Surgery, CD56 and CgA Expression Are Prognostic Factors for Patients With Locoregional Gastrin-Independent GNET

    Science.gov (United States)

    Li, Yuan; Bi, Xinyu; Zhao, Jianjun; Huang, Zhen; Zhou, Jianguo; Li, Zhiyu; Zhang, Yefan; Li, Muxing; Chen, Xiao; Hu, Xuhui; Chi, Yihebali; Zhao, Dongbing; Zhao, Hong; Cai, Jianqiang

    2016-01-01

    Abstract Gastrin-independent gastric neuroendocrine tumors (GNETs) are highly malignant. Radical resections and lymphadenectomy are considered to be the only possible curative treatment for these tumors. However, the prognosis of gastrin-independent GNETs is not well defined. In this study, we identified prognostic factors of locoregional gastrin-independent GNETs. All patients diagnosed with locoregional gastrin-independent GNETs between 2000 and 2014 were included in this retrospective study. Clinical characteristics, blood tests, pathological characteristics, treatments, and follow-up data of the patients were collected and analyzed. Of the 66 patients diagnosed with locoregional gastrin-independent GNETs, 57 (86.4%) received radical resections, 7 (10.6%) with palliative resection, 1 (1.5%) with gastrojejunostomy, and 1 (1.5%) with exploration surgeries. The median survival time for these patients was 19.0 months (interquartile range, 11.0–38.0). The 1-, 3-, and 5-year survival rates were 72%, 34%, and 28%, respectively. Multivariate analysis indicated that carcinoembryonic antigen (CEA) level (P = 0.04), radical resection (P = 0.04), and positive Cluster of Differentiation 56 (CD56) expression (P = 0.016) were significant prognostic factors on overall survival rate. Further univariate and multivariate analysis of 57 patients who received radical resections found that CgA expression (P = 0.35) and CEA level (P = 0.33) are independent prognostic factors. Gastrin-independent GNETs had poor prognosis. Serum CEA level, radical surgery, CD56 and CgA expression are markers to evaluate the survival of patients with locoregional gastrin-independent GNETs. PMID:27149478

  20. The value of F-18 fluorodeoxyglucose positron emission tomography/computed tomography in asymptomatic examinees with unexplained elevated blood carcinoembryonic antigen levels

    Energy Technology Data Exchange (ETDEWEB)

    Li, Wenfeng [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Department of Radiation Oncology, Wenzhou (China); Yin, Weiwei [The First Affiliated Hospital of Wenzhou Medical University, Division of PET/CT, Department of Radiology, Wenzhou (China); Ou, Rongying [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Department of Gynaecology and Obstetrics, Wenzhou (China); Chen, Ting; Xiong, Lingling; Xu, Yunsheng [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Department of Dermatovenereology, Wenzhou (China); Cheng, Dezhi; Xie, Deyao [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Department of Cardiothoracic Surgery, Wenzhou (China); Zheng, Xiangwu; Zhao, Liang [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Division of PET/CT, Department of Radiology, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Institutes of Intelligent and Molecular Imaging, Wenzhou (China)

    2016-04-15

    Cancer is still a clinical challenge, with many efforts invested in order to achieve timely detection. Unexplained elevated blood carcinoembryonic antigen levels are occasionally observed in an asymptomatic population and considered as a risk factor of cancers. The purpose of this study was to determine the validity of 18 F-fluorodeoxyglucose-positron emission tomography/computed tomography (F-18 FDG-PET/CT) for detecting cancer in an asymptomatic population with an unexplained elevation in blood carcinoembryonic antigen (CEA) levels. This retrospective study included a total of 1920 asymptomatic examinees conducted from August 2011 through September 2013. The participants underwent CEA assay and conventional medical imaging (CEA-conventional), or CEA assay and F-18 FDG-PET/CT (CEA-PET/CT). The validity of conventional medical imaging and CEA-PET/CT scanning for detecting cancer and early-stage cancer in an asymptomatic population with an unexplained elevation in blood CEA levels were evaluated. Sensitivity, specificity, cancer detection rate, missed cancer detection rate, early-stage cancer detection rate, and early-stage cancer ratio using the CEA-PET/CT scanning were 96.6 %, 100 %, 10.4 %, 0.4 %, 3.7 %, and 34.5 %, respectively. In contrast, the corresponding values obtained using the conventional medical imaging were 50.6 % (P < 0.0001), 100 % (P > 0.9999), 50.6 % (P < 0.0001), 99.9 % (P = 0.055), 2.6 % (P < 0.0001), 2.5 % (P = 0.04), 0.7 % (P = 0.0004), and 14.5 % (P = 0.002), respectively. The F-18 FDG-PET/CT scanning significantly improved the validity of the cancer detection program in the asymptomatic population with an unexplained elevation in CEA levels. (orig.)

  1. The value of F-18 fluorodeoxyglucose positron emission tomography/computed tomography in asymptomatic examinees with unexplained elevated blood carcinoembryonic antigen levels

    International Nuclear Information System (INIS)

    Cancer is still a clinical challenge, with many efforts invested in order to achieve timely detection. Unexplained elevated blood carcinoembryonic antigen levels are occasionally observed in an asymptomatic population and considered as a risk factor of cancers. The purpose of this study was to determine the validity of 18 F-fluorodeoxyglucose-positron emission tomography/computed tomography (F-18 FDG-PET/CT) for detecting cancer in an asymptomatic population with an unexplained elevation in blood carcinoembryonic antigen (CEA) levels. This retrospective study included a total of 1920 asymptomatic examinees conducted from August 2011 through September 2013. The participants underwent CEA assay and conventional medical imaging (CEA-conventional), or CEA assay and F-18 FDG-PET/CT (CEA-PET/CT). The validity of conventional medical imaging and CEA-PET/CT scanning for detecting cancer and early-stage cancer in an asymptomatic population with an unexplained elevation in blood CEA levels were evaluated. Sensitivity, specificity, cancer detection rate, missed cancer detection rate, early-stage cancer detection rate, and early-stage cancer ratio using the CEA-PET/CT scanning were 96.6 %, 100 %, 10.4 %, 0.4 %, 3.7 %, and 34.5 %, respectively. In contrast, the corresponding values obtained using the conventional medical imaging were 50.6 % (P < 0.0001), 100 % (P > 0.9999), 50.6 % (P < 0.0001), 99.9 % (P = 0.055), 2.6 % (P < 0.0001), 2.5 % (P = 0.04), 0.7 % (P = 0.0004), and 14.5 % (P = 0.002), respectively. The F-18 FDG-PET/CT scanning significantly improved the validity of the cancer detection program in the asymptomatic population with an unexplained elevation in CEA levels. (orig.)

  2. Avaliação dos valores sérico e pleural dos marcadores tumorais CEA, CYFRA21-1 e CA 15-3 em portadores de derrame pleural Evaluation of serum and pleural levels of the tumor markers CEA, CYFRA21-1 and CA 15-3 in patients with pleural effusion

    Directory of Open Access Journals (Sweden)

    Isabella Coimbra Wagner

    2007-04-01

    Full Text Available OBJETIVO: Dosar os marcadores tumorais antígeno carcinoembrionário (CEA, fragmento da citoqueratina 19 (CYFRA21-1 e antígeno glicosídico associado a tumor 15-3 (CA 15-3 em sangue e líquido pleural de portadores de derrames pleurais benignos e malignos, avaliando a sensibilidade de cada um deles nesses fluidos. MÉTODOS: Avaliamos prospectivamente 85 pacientes com derrame pleural. O estudo do líquido pleural obedeceu a critérios determinados pela literatura. A dosagem dos marcadores foi realizada por eletroquimioluminescência. A sensibilidade foi determinada sob a condição de que a especificidade fosse > 90%. RESULTADOS: Foram diagnosticados 36 casos malignos (42,4%, 30 benignos (35,3%; em 19 pacientes (22,3%, o diagnóstico foi inconclusivo. Nos casos malignos, os valores de CEA e CYFRA21-1 foram maiores no líquido pleural do que no sangue, fato não observado para o CA 15-3. Nos casos benignos, os valores do CYFRA21-1 foram maiores no líquido pleural do que no soro, enquanto que para o CEA e o CA 15-3, ocorreu o oposto. Todos os marcadores apresentaram diferença significativa entre os casos malignos e benignos, em líquido pleural e soro. Foi encontrada sensibilidade para CEA, CYFRA21-1 e CA 15-3 no líquido pleural de 69,4%, 69,4% e 66,7%, respectivamente e quando associados, foi 80,6%. No soro, a sensibilidade foi 57,1, 71,4 e 48,6% para CEA, CYFRA21-1 e CA 15-3, respectivamente, e quando associados, foi 77%. CONCLUSÃO: Os resultados sugerem que a utilização desses marcadores pode ser útil na diferenciação entre derrames pleurais malignos e benignos.OBJECTIVE: To determine the levels of the tumor markers carcinoembryonic antigen (CEA, cytokeratin 19 fragment (CYFRA21-1 and carbohydrate antigen 15-3 (CA 15-3 in the blood and pleural fluid of patients with benign or malignant pleural effusion, evaluating the sensitivity of each marker in these fluids. METHODS: We prospectively evaluated 85 patients with pleural effusion. The

  3. A prospective study of 2-[F-18] fluoro-2-deoxy-D-glucose/positron emission tomography scan, Tc-99m-labeled arcitumomab (CEA-scan), and blind second-look laparotomy for detecting colon cancer recurrence in patients with increasing carcinoembryonic antigen levels

    Czech Academy of Sciences Publication Activity Database

    Libutti, S. K.; Alexander, HR.; Choyke, P.; Bartlett, DL.; Bacharach, SL.; Whatley, M.; Jousse, F.; Eckelman, WC.; Kranda, Karel; Neumann, RD.; Carrasquillo, JA.

    2001-01-01

    Roč. 8, č. 10 (2001), s. 779-786. ISSN 1068-9265 R&D Projects: GA AV ČR KSK4055109 Keywords : carcinoembryonic antigen * positron emission tomography * FDG Subject RIV: FD - Oncology ; Hematology Impact factor: 3.308, year: 2001

  4. Duodenal Duplication Cyst with Profound Elevation of Intracystic Carbohydrate Antigen (CA 19-9 and Carcinoembryonic Antigen (CEA: A Rare but Important Differential in the Diagnosis of Cystic Tumours of the Pancreas

    Directory of Open Access Journals (Sweden)

    Kant J Shah

    2006-03-01

    Full Text Available The diagnosis of cystic tumours of the pancreas can be difficult. The principal effort is directed towards distinguishing benign post-inflammatory pseudocysts from true cystic tumours [1, 2]. Pointers that may help to identify a cystic tumour include the presence of a thick or irregular lining with septae on cross-sectional imaging [2]. Further, endoscopic ultrasound (EUS with linear EUS-guided aspiration can aid this process of differentiation as cyst fluid can be analysed for tumour markers, amylase and cytology [3, 4]. Elevated levels of intra-cyst tumour markers favour the diagnosis of a true cystic lesion [5]. It is important to make the distinction between post-inflammatory pseudocysts and cystic tumours as the natural history of these conditions is different and this is reflected in their different optimal management. Cystic lesions carry a varying risk of malignant transformation and most require resection [2]. Further, mistaking a cystic tumour for a pseudocyst can have disastrous consequences and lead to inappropriate drainage surgery [6]. In practice, cystic tumours and pseudocysts are the principal differential diagnoses to be assessed in a patient with a pancreatic cystic lesion; however a third rare possible differential is that of duplication cyst. These are rare congenital abnormalities of the gut characterized by the presence of smooth muscle in the wall and can be lined with gastrointestinal mucosa [7]. Little is known of the natural history and malignant potential of these lesions and as a result of their rarity, knowledge of their presentation accrues principally through single case studies. This paper reports a patient presenting with a pancreatic cystic lesion who underwent resection on the grounds of having a complex cyst with a profoundly elevated intra-cystic carbohydrate antigen (CA 19-9 level together with carcinoembryonic antigen (CEA. Final histology revealed a duodenal duplication cyst without malignancy. To the

  5. Relationship between serum carcinoembryonic antigen level and epidermal growth factor receptor mutations with the influence on the prognosis of non-small-cell lung cancer patients

    Directory of Open Access Journals (Sweden)

    Cai ZX

    2016-06-01

    Full Text Available Zuxun Cai Department of Thoracic Surgery, Henan Provincial Chest Hospital, Zhengzhou City, People’s Republic of China Objective: To investigate the relationship between serum carcinoembryonic antigen (CEA level and epidermal growth factor receptor (EGFR gene mutations in non-small-cell lung cancer (NSCLC patients and to analyze the influence of CEA level on postoperative survival time in lung cancer patients. Methods: A total of 296 patients who were treated in Thoracic Surgery Department of Henan Provincial Chest Hospital from September 2011 to September 2013 were recruited. The level of tumor markers, such as CEA, was determined before the surgery, and EGFR gene mutations were detected after surgery. Thereby, the relationship between tumor makers, including CEA, and EGFR mutation and its influence on prognosis could be investigated. Results: Among 296 patients, the positive rate of EGFR gene mutation was 37.84% (112/296; the mutation occurred more frequently in nonsmokers, adenocarcinoma patients, women, and patients aged <60 years (P<0.05. Both tumor markers and chemosensitivity indicators were related to the profile of EGFR mutations. Elevated squamous cell carcinoma and Cyfra21-1 as well as positively expressed ERCC1 were more common in patients with wild-type EGFR (P<0.05, whereas increased CEA level was observed more frequently in patients with EGFR gene mutation (P=0.012. The positive rate of EGFR gene mutations was higher as the serum CEA level increased, that is, the positive rate in patients with serum CEA level <5, 5–20, and >20 µg/L was 39.81%, 45.32%, and 65.47%, respectively (P=0.004. Logistic regression analysis showed that CEA level was an independent factor in predicting EGFR gene mutations, and serum CEA level was also an independent factor in affecting the prognosis of NSCLC patients, as the overall 2-year survival rate was 73.86% in elevated CEA group and 86.43% in normal group (P<0.01. Conclusion: The prognosis of

  6. Relationship between CT features and high preoperative serum carcinoembryonic antigen levels in early-stage lung adenocarcinoma

    International Nuclear Information System (INIS)

    Aim: To assess the relationship between thin-section computed tomography (CT) features of primary tumour and high preoperative serum carcinoembryonic antigen (CEA) levels that reportedly suggest poor prognoses in early-stage lung adenocarcinoma. Materials and methods: Two hundred and seventy-five consecutive patients who underwent resection of pathological stage I (T1–2aN0M0) adenocarcinomas with a maximum diameter of ≤3 cm (144 men, 131 women; mean age 67.8 years) were enrolled. CT features of the primary tumours and clinical characteristics of these patients were statistically evaluated to identify the factors associated with high serum CEA levels (>5 ng/ml). Results: Eighty-one patients (29.5%) had high serum CEA levels. In univariate analysis, lower ground-glass opacity ratio (p < 0.001), lower tumour shadow disappearance rate (TDR: the ratio of tumour area in mediastinal window to that of lung window, p < 0.001), presence of notch (p = 0.015), and coexistence with bullae or honeycomb cysts (p < 0.001) were observed more frequently in the group with high serum CEA levels than that of the group with normal levels. TDR [odds ratio (OR) 0.984; 95% confidence interval (CI): 0.976–0.993; p < 0.001] and coexistence with bullae or honeycomb cysts (OR = 3.08; 95% CI: 1.55–6.12; p = 0.001) remained significant, even after adjusting patients' age, gender, and smoking status. Conclusions: Adenocarcinomas with lower TDR and coexisting with bullae or honeycomb cysts are associated with high preoperative serum CEA levels. Although some CEA elevations may be due to benign pulmonary diseases, such tumours are suspected to have poor prognoses, even for early-stage diseases

  7. Combined detection of preoperative serum CEA, CA19-9 and CA242 improve prognostic prediction of surgically treated colorectal cancer patients

    OpenAIRE

    Wang, Jingtao; Xiao WANG; Yu, Fudong; Chen, Jian; Zhao, Senlin; Zhang, Dongyuan; YU, Yang; Liu, Xisheng; Tang, Huamei; Peng, Zhihai

    2015-01-01

    We assessed the prognostic significance of preoperative serum carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9) and carbohydrate antigen 242 (CA242) levels in surgically treated colorectal cancer patients. The relationship of preoperative serum CEA, CA19-9 and CA242 levels with disease characteristics was investigated in 310 patients. Correlation between tumor markers was investigated using Pearson correlation test. Univariate and multivariate survival analyses were used to s...

  8. Diagnostic value of combined measurement of serum CEA, AFP, CA19-9 and SA levels in patients with primary hepatic cancer

    International Nuclear Information System (INIS)

    Objective: To study the diagnostic value of combined measurement of serum CEA, AFP, CA19-9 and SA levels for primary hepatic cancer. Methods: Serum CEA, AFP, CA19-9 (with RIA) SA (with biochemistry) levels were measured in 36 patients with primary hepatic cancer and 35 controls. Results: As a single tumor marker for diagnosis, the sensitivity of CEA, AFP, CA19-9, SA was 33.3%, 69.4%, 58.5% and 61.1% respectively, the specificity was 60.4%, 81.3% 68.8%, 64.4% respectively. With combined detection of the four markers and assuming two or more markers positive as positive, the sensitivity would be 94.4% and specificity would be 95.8%. Conclusion: Combined measurement of serum CEA, AFP, CA19-9 and SA levels is of clinical usefulness for diagnosis of primary hepatic cancer. (authors)

  9. Bivalent fragment of the ior-CEA1 antibody. A challenge to the positive CEA tumors radioimmunotherapy

    International Nuclear Information System (INIS)

    The directed radiotherapy of the solid tumors with fragments recombinants of radiolabelled antibodies is a topic of current investigation, so much at preclinical level as clinical. This work describes the preclinical characterization of a new fragment type diabody of the AcMo ior CEA1 that has been labelled with 131 I for their use in the diagnosis and the therapy of CEA positive tumors. The radiolabelling methodology used allows the incorporation of more than 90% of the radio iodine to the molecule without committing the capacity of recognition of its antigen significantly. The combination of the favourable properties pharmacy kinetic and high selective accumulation in the tumor, they make of the diabody anti CEA an appropriate candidate for the radioimmunodiagnosis and the radioimmunotherapy of tumors that expresses CEA (Author)

  10. Pretreatment carcinoembryonic antigen level is a risk factor for para-aortic lymph node recurrence in addition to squamous cell carcinoma antigen following definitive concurrent chemoradiotherapy for squamous cell carcinoma of the uterine cervix

    International Nuclear Information System (INIS)

    To identify pretreatment carcinoembryonic antigen (CEA) levels as a risk factor for para-aortic lymph node (PALN) recurrence following concurrent chemoradiotherapy (CCRT) for cervical cancer. From March 1995 to January 2008, 188 patients with squamous cell carcinoma (SCC) of the uterine cervix were analyzed retrospectively. No patient received PALN irradiation as the initial treatment. CEA and squamous cell carcinoma antigen (SCC-Ag) were measured before and after radiotherapy. PALN recurrence was detected by computer tomography (CT) scans. We analyzed the actuarial rates of PALN recurrence by using Kaplan-Meier curves. Multivariate analyses were carried out with Cox regression models. We stratified the risk groups based on the hazard ratios (HR). Both pretreatment CEA levels ≥ 10 ng/mL and SCC-Ag levels < 10 ng/mL (p < 0.001, HR = 8.838), SCC-Ag levels ≥ 40 ng/mL (p < 0.001, HR = 12.551), and SCC-Ag levels of 10-40 ng/mL (p < 0.001, HR = 4.2464) were significant factors for PALN recurrence. The corresponding 5-year PALN recurrence rates were 51.5%, 84.8%, and 27.5%, respectively. The 5-year PALN recurrence rate for patients with both low (< 10 ng/mL) SCC and CEA was only 9.6%. CEA levels ≥ 10 ng/mL or SCC-Ag levels ≥ 10 ng/mL at PALN recurrence were associated with overall survival after an isolated PALN recurrence. Pretreatment CEA levels ≥ 10 ng/mL were also associated with survival after an isolated PALN recurrence. Pretreatment CEA ≥ 10 ng/mL is an additional risk factor of PALN relapse following definitive CCRT for SCC of the uterine cervix in patients with pretreatment SCC-Ag levels < 10 ng/mL. More comprehensive examinations before CCRT and intensive follow-up schedules are suggested for early detection and salvage in patients with SCC-Ag or CEA levels ≥ 10 ng/mL

  11. Serum tetranectin is an independent prognostic marker in colorectal cancer and weakly correlated with plasma suPAR, plasma PAI-1 and serum CEA

    DEFF Research Database (Denmark)

    Høgdall, Claus K; Christensen, Ib J; Stephens, Ross W;

    2002-01-01

    activator (uPAR) and carcinoembryonic antigen (CEA). Significantly shorter survival was found for patients with TN levels below a cut-off point of 7.5 mg/l compared to patients with levels above, as illustrated by Kaplan-Meier curves. By Cox analyses, log TN, log soluble uPAR as well as log CEA were found...

  12. Evaluation of the use of decision-support software in carcino-embryonic antigen (CEA-based follow-up of patients with colorectal cancer

    Directory of Open Access Journals (Sweden)

    Verberne Charlotte J

    2012-03-01

    Full Text Available Abstract Background The present paper is a first evaluation of the use of "CEAwatch", a clinical support software system for surgeons for the follow-up of colorectal cancer (CRC patients. This system gathers Carcino-Embryonic Antigen (CEA values and automatically returns a recommendation based on the latest values. Methods Consecutive patients receiving follow-up care for CRC fulfilling our in- and exclusion criteria were identified to participate in this study. From August 2008, when the software was introduced, patients were asked to undergo the software-supported follow-up. Safety of the follow-up, experiences of working with the software, and technical issues were analyzed. Results 245 patients were identified. The software-supported group contained 184 patients; the control group contained 61 patients. The software was safe in finding the same amount of recurrent disease with fewer outpatient visits, and revealed few technical problems. Clinicians experienced a decrease in follow-up workload of up to 50% with high adherence to the follow-up scheme. Conclusion CEAwatch is an efficient software tool helping clinicians working with large numbers of follow-up patients. The number of outpatient visits can safely be reduced, thus significantly decreasing workload for clinicians.

  13. Diagnostic Value of Cerebrospinal Fluid Level of Carcinoembryonic Antigen in Patients with Leptomeningeal Carcinomatous Metastasis

    OpenAIRE

    Kang, Sung Jin; Kim, Kwang Soo; Ha, Yoon Suk; Huh, So Young; Lee, Ji Hyun; Kim, Jong Kuk; Kim, Min Jeong

    2010-01-01

    Background and Purpose Multifocal seeding of the leptomeninges by malignant cells, which is usually referred to as leptomeningeal carcinomatous metastasis, produces substantial morbidity and mortality. The diagnosis of leptomeningeal metastasis is usually established by cerebrospinal fluid (CSF) investigation, including cytology, cell counts, protein, glucose, and a tumor marker such as carcinoembryonic antigen (CEA). This study examined the diagnostic value of CEA in the CSF. Methods We meas...

  14. The importance of preoperative elevated serum levels of CEA and CA15-3 in patients with breast cancer in predicting its histological type.

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    J Słonina

    2010-06-01

    Full Text Available It is not known whether in patients with breast cancer the occurrence of elevated serum tumour markers depends on its histological type. The aim of the study was to assess relationship between breast cancer histological type and the presence of increased serum levels of CEA and CA 15-3. The study population was 428 patients (all women, mean age 52.5 years, treated at The Department of Surgery of Wroclaw Medical University from 2005 to 2008 due to breast cancer. All of them had their preoperative CA 15-3 and CEA serum concentrations measured. According to the TNM system, 21% of patients were in stage I, 32.5% in stage II, 46.5% in stage III of the disease. In patients with ductal type of the cancer the elevated serum levels of CEA and CA 15-3 were observed in 48.7% and 42.2%, in lobular type in 42.4% and 52.5%, and in non-ductal/tubular types in 48.1% and 40.4% (p=N/S. Stepwise logistic regression analyses showed that ductal breast cancer is related to elevated CEA and normal CA 15-3 serum levels. The histological types of breast cancer are not significantly related to elevated serum levels of CEA and/or CA 15-3.

  15. Construction of CEA siRNA expression vector and its inhibitory effects on the expression of CEA in EC9706 cells%CEA siRNA载体的构建和转染人食管癌EC9706细胞的沉默作用

    Institute of Scientific and Technical Information of China (English)

    Guoqiang Zhao; Sajia Sun; Hong Zheng; Long Feng; Hongyan Yang; Ziming Dong

    2008-01-01

    Objective: To construct the small interfering RNA (siRNA) expression vector of carcino-embryonic antigen (CEA) and inhibit the expression of CEA in EC9706 cells by RNA interference. Methods: Two pairs of oligonucleotide sequences were designed and synthesized according to the encoding sequence of mRNA of CEA. The annealed oligonucleotide fragments were cloned into pRNAT-U6.2 expression vector and identified by sequencing. The recombinant plasmid pRNAT-U6.2-CEA was transfected into EC9706 cells. The expression of CEA in the stable transfected cells was assayed by real time PCR and Western blot. Results: DNA sequencing showed that the oligonucleotide fragments were correctly inserted into pRNAT-U6.2 vector, and CEA expression in the transfected cells was down-regulated significantly by pRNAT-U6.2-CEA at both the mRNA and protein levels. Conclusion: The siRNA expression vector of CEA is successfully constructed and inhibits CEA expression in EC9706 cells. This facilitates further studies of the function of CEA at the molecular level.

  16. Improvements on Low Level Activity Gamma Measurements and X-ray Spectrometry at the CEA-MADERE Measurement Platform

    Science.gov (United States)

    Sergeyeva, Victoria; Domergue, Christophe; Destouches, Christophe; Girard, Jean Michel; Philibert, Hervé; Bonora, Jonathan; Thiollay, Nicolas; Lyoussi, Abdallah

    2016-02-01

    The CEA MADERE platform (Measurement Applied to DosimEtry in REactors) is a part of the Instrumentation Sensors and Dosimetry Laboratory (LDCI). This facility is dedicated to the specific activity measurements of solid and radioactive samples using Gamma and X-ray spectrometry. MADERE is a high-performance facility devoted to neutron dosimetry for experimental programs performed in CEA and for the irradiation surveillance programmes of PWR vessels. The MADERE platform is engaged in a continuous improvement process. Recently, two High Efficiency diodes have been integrated to the MADERE platform in order to manage the accurate low level activity measurements (few Bq per sample). This new equipment provides a good level of efficiency over the energy range from 60 keV to 2 MeV. The background continuum is reduced due to the use of a Ultra Low Background (ULB) lead shielding. Relative and absolute X-ray measurement techniques have been improved in order to facilitate absolute rhodium activity measurement (Rh103m) on solid samples. Additional efforts have been made to increase the accuracy of the relative niobium (Nb93m) activity measurement technique. The way of setting up an absolute measurement method for niobium is under investigation. After a presentation of the MADERE's measurement devices, this paper focuses on the technological options taken into account for the design of high efficiency measurement devices. Then, studies performed on X-ray measurement techniques are presented. Some details about the calculation of uncertainties and correction factors are also mentioned. Finally, future research and development axes are exposed.

  17. Improvements on Low Level Activity Gamma Measurements and X-ray Spectrometry at the CEA-MADERE Measurement Platform

    Directory of Open Access Journals (Sweden)

    Sergeyeva Victoria

    2016-01-01

    Full Text Available The CEA MADERE platform (Measurement Applied to DosimEtry in REactors is a part of the Instrumentation Sensors and Dosimetry Laboratory (LDCI. This facility is dedicated to the specific activity measurements of solid and radioactive samples using Gamma and X-ray spectrometry. MADERE is a high-performance facility devoted to neutron dosimetry for experimental programs performed in CEA and for the irradiation surveillance programmes of PWR vessels. The MADERE platform is engaged in a continuous improvement process. Recently, two High Efficiency diodes have been integrated to the MADERE platform in order to manage the accurate low level activity measurements (few Bq per sample. This new equipment provides a good level of efficiency over the energy range from 60 keV to 2 MeV. The background continuum is reduced due to the use of a Ultra Low Background (ULB lead shielding. Relative and absolute X-ray measurement techniques have been improved in order to facilitate absolute rhodium activity measurement (Rh103m on solid samples. Additional efforts have been made to increase the accuracy of the relative niobium (Nb93m activity measurement technique. The way of setting up an absolute measurement method for niobium is under investigation. After a presentation of the MADERE's measurement devices, this paper focuses on the technological options taken into account for the design of high efficiency measurement devices. Then, studies performed on X-ray measurement techniques are presented. Some details about the calculation of uncertainties and correction factors are also mentioned. Finally, future research and development axes are exposed.

  18. Early postoperative serum carcinoembryonic antigen levels in patients operated for colorectal carcinoma. A new method for following-up

    International Nuclear Information System (INIS)

    Background. The only method of treatment offering a favourable prognosis for colorectal carcinoma is radical resection of the part of the colon or rectum including the pertaining lymph nodes and eventual radical removal of metastases. But even such presumably curative surgery does not warrant full recovery of all operated patients as recurrences are frequent and according to most analyses 5-year survival is lower than 50%. Therefore, additional treatment is attempted in some patients. Various prognostic factors of disease recurrence are helpful. One such prognostic sign is serum carcinoembryonic antigen (CEA) level measured soon after surgery. Conclusions. All patients with radical R0 resection, according to their postoperative serum CEA levels and the CEA half-life fall into three groups: CEAR0, CEAR1 and CEAR2 resected patients. A statistically significant difference regarding survival and number of recurrences was noted among patients categorized by the stage of disease, particularly between the three groups of patients and the group having been undergone presumably curative surgery. (author)

  19. Serum levels of fetal antigen 1 in extreme nutritional States

    DEFF Research Database (Denmark)

    Andries, Alin; Niemeier, Andreas; Støving, Rene K; Abdallah, Basem M; Wolf, Anna-Maria; Hørder, Kirsten; Kassem, Moustapha

    2012-01-01

    Objective. Recent data suggest that fetal antigen (FA1) is linked to disorders of body weight. Thus, we measured FA1 serum levels in two extreme nutritional states of morbid obesity (MO) and anorexia nervosa (AN) and monitored its response to weight changes. Design. FA1 and insulin serum concentr......Objective. Recent data suggest that fetal antigen (FA1) is linked to disorders of body weight. Thus, we measured FA1 serum levels in two extreme nutritional states of morbid obesity (MO) and anorexia nervosa (AN) and monitored its response to weight changes. Design. FA1 and insulin serum...

  20. Clinical significant of combined determination of serum CEA and carbohydrate antigen tumor markers in 85 patients with lung adenocarcinoma%肺腺癌患者血清CEA和糖链抗原肿瘤标记物联合测定的临床意义

    Institute of Scientific and Technical Information of China (English)

    沈粉秧

    2014-01-01

    Objective To explore the clinical significant of combined determination of serum CEA and car-bohydrate antigen (CA125, CA199, CA153) tumor markers in patients with lung adenocarcinoma. Methods The serum CEA and carbohydrate antigen (CA125, CA199 and CA153) levels were determined with chemilumescence immunoassay in 85 patients with lung adenocarcinoma, 35 patients with lung squanous carcinoma, 42 patients with small cell lung cancer and 49 patients with benign lung disease. Results In 162 patients with lung cancer, the ser-um CEA and carbohydrate antigen levels were significantly higher in 85 patients with lung adenocarcinoma, 35 pa-tients with lung squanous carcinoma and 42 patients with small cell lung cancer than those in 49 patients with benign lung disease (P<0. 001) and the level of 85 patients with lung adenocarcinoma was the highest. So did the serum carbohydrate antigen levels. The sensitivity (95. 29%) and accuracy (83. 76%) in the four tumor markers combined determination were significantly higher than those in single item ( P all<0. 01 ) , and the specificity was 75. 23%. Conclusion The combined detection of serum CEA and carbohydrate antigen levels is helpful for the diagnosis of lung adenocarcinoma, with higher sensitivity and accuracy.%目的:为了探讨85例肺腺癌患者血清CEA和糖链抗原肿瘤标记物水平联合测定的临床意义。方法化学发光免疫分析测定85例肺腺癌、35例肺鳞癌、42例小细胞肺癌和49例良性肺部疾病血清CEA和糖链抗原(CA125、CA199和CA153)水平,并进行了比较性研究。结果162例肺癌患者中,85例肺腺癌、35例肺鳞癌、42例小细胞肺癌患者血清CEA水平较之49例良性肺部疾病明显增高( P<0.001、P<0.01和P<0.001),以肺腺癌增高最为明显。血清糖链抗原(CA125、CA199和 CA153)水平亦然,也以肺腺癌血清CA125、CA199和CA153水平增高最为显著。四项肿瘤标记物的联合测定对85例肺腺癌的敏感性为95.29

  1. Relationship between peripheral and mesenteric serum levels of CEA and CA 242 with staging and histopathological variables in colorectal adenocarcinoma Níveis séricos periféricos e mesentéricos de CEA e CA 242, estadiamento e variáveis histopatológicas no adenocarcinoma colorretal

    Directory of Open Access Journals (Sweden)

    Mauro Lamelas Cardoso

    2009-10-01

    Full Text Available PURPOSE: To compare histopathological variables and staging in colorectal adenocarcinoma cases with CEA and CA 242 in peripheral and mesenteric blood. METHODS: In 169 individuals underwent surgery for colorectal cancer, CEA and CA 242 were analyzed and compared to mesenteric and peripheral blood and correlated with macroscopic tumor's morphology and size, degree of cell differentiation, venous, neural and lymphatic involvement and TNM classification. RESULTS: There was a difference between the mesenteric (M and peripheral (P serum levels of CEA (p=0.020. Higher levels of markers were correlated with venous invasion CEA (P p=0.013, CEA (M p=0.05, CA 242 (M p=0.005 and CA 242 (P p=0.038; with advanced staging CEA (P OBJETIVO: Comparar variáveis histopatológicas e graus de estadiamento do adenocarcinoma colorretal com níveis sanguíneos periféricos e mesentéricos de CEA e CA-242. MÉTODOS: Em 169 doentes submetidos ao tratamento cirúrgico por adenocarcinoma colorretal, CEA e CA-242 foram analisados e comparados quanto aos níveis sanguíneos periféricos e mesentéricos e correlacionados com o tamanho e a morfologia macroscópica do tumor, grau de diferenciação celular, invasões venosa, linfática, neural e a classificação TNM. RESULTADOS: Verificou-se diferença significante entre o nível sérico mesentérico e periférico de CEA (p= 0,02. Níveis séricos mais elevados dos marcadores foram observados e correlacionados com invasão venosa, CEA (P p=0,013, CEA(M, p=0,05, CA-242 (M p=0,005 e CA-242 (P p=0,038. Grau de estadiamento TNM avançado foi associado com CEA(P < CEA(M p<0,05, CA-242(P < CA-242(M p<0,05. Nas maiores dimensões tumorais constatou-se CEA(P < CEA(M p=0,001 e CA 242 (P < CA 242 (M (p < 0.001. O CA 242 periférico e mesentérico aumentados associaram-se com a invasão neural, p=0.014 e p=0.003, respectivamente. CONCLUSÕES: O nível sérico mesentérico de CEA é superior ao nível sérico periférico. Os níveis s

  2. The relationship between chemotherapy tolerance and CA125、CEA levels of patients with advanced NSCLC%晚期NSCLC患者血清CA125、CEA水平与化疗耐受的关系及意义

    Institute of Scientific and Technical Information of China (English)

    张秀萍; 熊新华; 瞿少刚

    2011-01-01

    目的:糖类抗原125(carbohydrate antigen,CAl25)、癌胚抗原(carcinoembryonic antigen,CEA)是最早被应用于临床的肿瘤标志物.对于肺癌患者,监测它们主要用于早期诊断和疗效评价,且两者联合其他指标具有较高的灵敏度和准确性,但其对疗效的具体影响目前还不清楚.为此我们研究了晚期非小细胞肺癌(NSCLC)患者血清CA125、CEA水平及其与化疗耐受次数的关系,从化疗耐受的角度初步探讨其对化疗的影响.方法:应用回顾性分析,观察274例晚期NSCLC患者化疗前血清CA125水平、化疗耐受次数和其中110例患者血清CEA水平,计算患者CA125和CEA阳性率,并将化疗耐受次数与化疗前血清CA125、CEA水平用SPAA10.0软件进行统计学处理.结果:部分晚期NSCLC患者CA125、CEA水平升高,且CA125阳性率(62.4%)高于CEA阳性率(40%);化疗前CA125水平对NSCLC化疗的耐受次数有统计学意义(P0.05).结论:对于晚期NSCLC患者,CA125具有更高的灵敏度,且其化疗前水平在一定程度上可预测患者的化疗耐受,监测CA125比CEA更有利于对患者进行化疗耐受评估,指导临床选择治疗手段.%Objective CA125 and CEA are the first tumor markers to be used for clinical purpose. For patients with lung cancer, These markers are mainly used for early diagnosis and monitoring the efficacy of therapy, and there be high sensitivity and accuracy in combination with other tumor markers ,but their detailed therapeutic effects are unclear. So we study the relationship between chemotherapy tolerance and CA125、CEA levels of patients with advanced NSCLC to find how their impact on chemotherapy from a chemotherapeutic tolerance perspective. Methods By retrospective analysis, we observe the serum CA125 level of 274 advanced NSCLC patients before chemotherapy, tracking the number of their tolerance of chemotherapy, and gather the level of serum CEA of 110 cases among them. Then we calculated the positive rate of CA

  3. Combined effects of protein kinase inhibitors and 5-fluorouracil on CEA expression in human colon cancer cells.

    Science.gov (United States)

    Prete, Salvatore Pasquale; Rossi, Lorena; Correale, Pier Paolo; Turriziani, Mario; Baier, Susanne; Tamburrelli, Giuliana; De Vecchis, Liana; Bonmassar, Enzo; Aquino, Angelo

    2005-08-01

    Previous studies showed that 5-fluorouracil (5-FU) and Staurosporine (ST), a protein kinase inhibitor (PKI), were able to increase the expression of carcinoembryonic antigen (CEA) in human colon cancer cells. In the present study, we examined the in vitro effects of five PKIs, i.e. ST, 1-5-isoquinolinyl-sulfonyl-2-methylpiperazine (H-7), bisindolylmaleimide-I (BIS), Genistein (GEN), and Herbimycin A (HERB) alone or in combination with 5-FU on CEA expression. C22-20, a clonal subline, derived from colon cancer HT-29 line, selected for low expression of CEA, was used in our experimental model. Among the PKIs tested, only ST, at non-toxic concentrations of 5 nM, was capable of increasing the level of CEA. The other PKIs did not modify CEA expression when used either alone or in combination with 5-FU. Flow cytometric analysis showed that treatment of cells with 5-FU + ST resulted in a synergistic increase of CEA expression, being higher than that obtainable with both agents alone. Moreover, the increase of CEA expression occurred not only in membrane fractions but also in cytosolic compartments, as indicated by Western blot analysis. The present study suggests that ST-mediated induction of CEA expression in cancer cells is PKC independent and could be of potential clinical interest for the development of new diagnostic and/or immunotherapeutic approaches. PMID:15967383

  4. Carcinoembryonic antigen continuous epitopes determined by the spot method.

    Science.gov (United States)

    Solassol, I; Granier, C; Pèlegrin, A

    2001-01-01

    Carcinoembryonic antigen (CEA) is a heavily glycosylated tumor-associated protein with an N-A1-B1-A2-B2-A3-B3 domain structure. Circulating CEA immunoassays are used for monitoring digestive cancer patients, and radiolabeled anti-CEA monoclonal antibodies (MAb) are used for the diagnosis and therapy of CEA-positive tumors. The five major nonoverlapping epitopes (Gold 1-5) have been broadly correlated with the domain organization, but there is no precise localization of the epitopes at the sequence level. In an attempt to identify the peptide sequences corresponding to the five Gold epitopes on the CEA molecule, we prepared a set of 227 overlapping fifteen-mer peptides corresponding to the complete CEA sequence with the SPOT method. Using five high affinity MAbs directed against the five CEA Gold epitopes, we demonstrated that none of these epitopes could be mimicked by a fifteen-mer peptide sequence. However, using rabbit and goat anti-CEA sera, we identified six major continuous antigenic regions. All are included in the Ig-like domains of the CEA: two in the A1 domain (residues 120-134 and 153-164), one each in the A2 (329-337) and A3 domains (508-513), one at the junction between the A3 and B3 domains (553-561) and one in the B3 domain (565-573). A very homologous sequence (common residues VSPRL) was mapped in each of the three A domains. Thus, in terms of occurrence of continuous epitopes, the Ig-like domains A1, A2, A3 and B3 seem to be the most antigenic parts of CEA. These peptide sequences should be good candidates for the future development of site-specific anti-CEA MAbs. PMID:11275797

  5. Antigen

    Science.gov (United States)

    An antigen is any substance that causes your immune system to produce antibodies against it. This means your immune ... and is trying to fight it off. An antigen may be a substance from the environment, such ...

  6. Utility of endoscopic ultrasound, cytology and fluid carcinoembryonic antigen and CA 19-9 levels in pancreatic cystic lesions

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    AIM: To assess the diagnostic accuracy of endoscopic ultrasound (EUS), fluid tumor markers and cytology in distinguishing benign from (pre)malignant pancreatic cystic lesions.METHODS: 46 consecutive patients, referred to a gastroenterologist and surgeon for a symptomatic or incidental pancreatic cyst, were reviewed. EUS, cytology,and carcinoembryonic antigen (CEA) and carbohydrate antigen (CA 19-9) levels were compared with the final diagnosis, based on surgical pathology and/or imaging follow-up of at least 12 mo. Cysts were classified as benign (pseudocyst, serous cystadenoma) or malignant/pre-malignant (mucinous cystic neoplasm). Receiveroperator characteristics (ROC) curve analysis was performed.RESULTS: The mean age was 56 years; 29% were male and median cyst diameter was 3 cm. Final outcome was obtained in 41 (89%) patients. Twenty-three (56%) of these 41 had surgical pathology. Twenty-three (56%)had benign lesions and 18 (44%) had malignant/premalignant lesions. Sensitivity, specificity and positive and negative predictive value of EUS alone to distinguish benign from malignant/premalignant pancreatic cystic lesions were 50%, 56%, 36% and 54% and for cytology were 71%, 96%, 92% and 85%, respectively. The corresponding values for the ROC-derived ideal cutoffs were 75%, 90%, 75%, 90% for CA 19-9 (> 37 U/mL)and 70%, 85%, 79% and 78% for CEA (> 3.1 ng/mL).Subgroup analysis of those with surgical pathology yielded almost identical performance and cutoffs.CONCLUSION: Cytology and cyst fluid tumor marker analysis is a very useful tool in distinguishing benign from (pre)malignant pancreatic cystic lesions.

  7. The great Cea actors

    International Nuclear Information System (INIS)

    With the Claude Frejacques life it is a Cea passage that we find. He began the studies on uranium isotope separation. He developed and saw to a successful conclusion these researches at Cea during twenty five years and extended his sphere of operations to the whole fuel cycle, from upstream to downstream. Director of research at the Cea, he was also D.G.R.S.T. director and during eight years President of the C.N.R.S. (N.C.)

  8. Prognostic value of monitoring tumour markers CA 15-3 and CEA during fulvestrant treatment

    International Nuclear Information System (INIS)

    At many centres tumour markers are used to detect disease recurrence and to monitor response to therapy in patients with advanced disease, although the real value of serial observation of marker levels remains disputed. In this study, we evaluated the prognostic value of tumour markers for predicting response (partial response [PR], stable disease [SD] ≥ 6 months), de novo disease progression (PD) and secondary PD in patients receiving fulvestrant ('Faslodex') 250 mg/month for the treatment of metastatic breast cancer (MBC). Changes in cancer antigen 15–3 (CA 15-3) and carcinoembryonic antigen (CEA) were prospectively monitored (monthly) and were also evaluated for the 3 months preceding secondary PD. Data from 67 patients with previously treated MBC participating in a Compassionate Use Programme were analysed. In patients with a PR (n = 7 [10.4%]), a non-significant increase in CA 15-3 occurred during the first 6 months of treatment; CEA was significantly reduced (P = 0.0165). In patients with SD ≥ 6 months (n = 28 [41.8%]), both CA 15-3 (P < 0.0001) and CEA (P = 0.0399) levels increased significantly after 6 months treatment. In those experiencing de novo PD (n = 32 [47.8%]), CA 15-3 increased significantly (P < 0.0001) after 4 months; CEA also increased significantly (P = 0.0002) during the same time period. Both CA 15-3 (P < 0.0001) and CEA (P < 0.0001) increased significantly in the 3 months preceding secondary PD. CA 15-3 increases in patients progressing on fulvestrant but may also increase in those experiencing clinical benefit; this should not be taken as a sign of PD without verification. Overall, both CA 15-3 and CEA appear to be poor prognostic markers for determining progression in patients receiving fulvestrant

  9. Independent prognostic value of preoperative serum markers CA 242, specific tissue polypeptide antigen and human chorionic gonadotrophin beta, but not of carcinoembryonic antigen or tissue polypeptide antigen in colorectal cancer.

    OpenAIRE

    Carpelan-Holmström, M; Haglund, C.; Lundin, J; Alfthan, H.; Stenman, U H; Roberts, P. J.

    1996-01-01

    The prognostic value of preoperative serum concentrations of carcinoembryonic antigen (CEA), CA 242, tissue polypeptide antigen (TPA), specific tissue polypeptide antigen (TPS) and human chorionic gonadotrophin beta (hCG beta) in 251 patients with colorectal cancer (39 Dukes' A, 98 Dukes' B, 56 Dukes' C and 58 Dukes' D) was investigated. When using the cut-off levels recommended for diagnostic purposes, there was a significantly longer overall survival in patients with low tumour marker level...

  10. CEA Annual report 2007

    International Nuclear Information System (INIS)

    The CEA, a prominent player in research development and innovation, is active in three main domains: energy, health care and information technology, defense and security. This annual report presents the CEA activities for the year 2007 in these three main areas: science and technology working for nuclear deterrence and global security, the energies without greenhouse effect gases emission against the climatic change, researches in the information sciences and technologies for a better communication and health. The CEA safety, organization, communication and international relations are also presented. (A.L.B.)

  11. CEA financial report 2007

    International Nuclear Information System (INIS)

    This document provides financial data on the CEA for the year 2007. The management report (budget, resources, expenditures) and the accounting are detailed. The main management events of the year 2007 are presented. (A.L.B.)

  12. Carcinoembryonic antigen promotes colorectal cancer progression by targeting adherens junction complexes

    Energy Technology Data Exchange (ETDEWEB)

    Bajenova, Olga, E-mail: o.bazhenova@spbu.ru [Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg 199034 (Russian Federation); Department of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg 199034 (Russian Federation); Department of Surgery and Biomedical Sciences, Creighton University, Omaha, NE 68178 (United States); Chaika, Nina [Department of Surgery and Biomedical Sciences, Creighton University, Omaha, NE 68178 (United States); Tolkunova, Elena; Davydov-Sinitsyn, Alexander [Institute of Cytology, Russian Academy of Sciences, St. Petersburg 194064 (Russian Federation); Gapon, Svetlana [Boston Children' s Hospital, Boston, MA 02115 (United States); Thomas, Peter [Department of Surgery and Biomedical Sciences, Creighton University, Omaha, NE 68178 (United States); O’Brien, Stephen [Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg 199034 (Russian Federation)

    2014-06-10

    Oncomarkers play important roles in the detection and management of human malignancies. Carcinoembryonic antigen (CEA, CEACAM5) and epithelial cadherin (E-cadherin) are considered as independent tumor markers in monitoring metastatic colorectal cancer. They are both expressed by cancer cells and can be detected in the blood serum. We investigated the effect of CEA production by MIP101 colorectal carcinoma cell lines on E-cadherin adherens junction (AJ) protein complexes. No direct interaction between E-cadherin and CEA was detected; however, the functional relationships between E-cadherin and its AJ partners: α-, β- and p120 catenins were impaired. We discovered a novel interaction between CEA and beta-catenin protein in the CEA producing cells. It is shown in the current study that CEA overexpression alters the splicing of p120 catenin and triggers the release of soluble E-cadherin. The influence of CEA production by colorectal cancer cells on the function of E-cadherin junction complexes may explain the link between the elevated levels of CEA and the increase in soluble E-cadherin during the progression of colorectal cancer. - Highlights: • Elevated level of CEA increases the release of soluble E-cadherin during the progression of colorectal cancer. • CEA over-expression alters the binding preferences between E-cadherin and its partners: α-, β- and p120 catenins in adherens junction complexes. • CEA produced by colorectal cancer cells interacts with beta-catenin protein. • CEA over-expression triggers the increase in nuclear beta-catenin. • CEA over-expression alters the splicing of p120 catenin protein.

  13. Carcinoembryonic antigen promotes colorectal cancer progression by targeting adherens junction complexes

    International Nuclear Information System (INIS)

    Oncomarkers play important roles in the detection and management of human malignancies. Carcinoembryonic antigen (CEA, CEACAM5) and epithelial cadherin (E-cadherin) are considered as independent tumor markers in monitoring metastatic colorectal cancer. They are both expressed by cancer cells and can be detected in the blood serum. We investigated the effect of CEA production by MIP101 colorectal carcinoma cell lines on E-cadherin adherens junction (AJ) protein complexes. No direct interaction between E-cadherin and CEA was detected; however, the functional relationships between E-cadherin and its AJ partners: α-, β- and p120 catenins were impaired. We discovered a novel interaction between CEA and beta-catenin protein in the CEA producing cells. It is shown in the current study that CEA overexpression alters the splicing of p120 catenin and triggers the release of soluble E-cadherin. The influence of CEA production by colorectal cancer cells on the function of E-cadherin junction complexes may explain the link between the elevated levels of CEA and the increase in soluble E-cadherin during the progression of colorectal cancer. - Highlights: • Elevated level of CEA increases the release of soluble E-cadherin during the progression of colorectal cancer. • CEA over-expression alters the binding preferences between E-cadherin and its partners: α-, β- and p120 catenins in adherens junction complexes. • CEA produced by colorectal cancer cells interacts with beta-catenin protein. • CEA over-expression triggers the increase in nuclear beta-catenin. • CEA over-expression alters the splicing of p120 catenin protein

  14. Neurocysticercosis: relationship between Taenia antigen levels in CSF and MRI

    Energy Technology Data Exchange (ETDEWEB)

    Abraham, Ronaldo, E-mail: rnabraham@uol.com.b [University of Taubate (UNITAU), Taubate, SP (Brazil). Medicine Dept.; Livramento, Jose Antonio; Machado, Luis dos Ramos [University of Sao Paulo (USP), Sao Paulo, SP (Brazil). Medical School. Neurology Dept.; Leite, Claudia da Costa [University of Sao Paulo (USP), Sao Paulo, SP (Brazil). Medical School. Radiology Dept.; Pardini, Alessandra Xavier; Vaz, Adelaide Jose [University of Sao Paulo (USP), Sao Paulo, SP (Brazil). Biomedical Science Institute. Immunology Dept.

    2010-02-15

    Objective: to determine the relationship between Taenia antigen (TA) detection in cerebrospinal fluid (CSF) and magnetic resonance imaging (MRI) findings in patients with definite diagnosis of neurocysticercosis (NC). Method: sixty-three patients with definite diagnosis of NC were submitted to a MRI of the brain, and to a CSF examination, with a meticulous search for TA by ELISA. Results: TA detection was positive in 36 patients (57.1%). A total of 836 lesions were analyzed, greatly within the cerebral parenchyma (98.7 of the lesions). Intact or non-degenerating cysts were the most common evolutive phase observed (50.4% of all lesions), 22.1% were degenerating cysts and 19.5% calcified cysts. We observed a significant relationship between TA levels detected and the total number of lesions and the number of non-degenerating cysts, but not with calcified lesions. Conclusion: according to our results, we propose at least four important types of contribution: TA detection may allow etiologic diagnosis in transitional phases of NC, with non-characteristic images; in final stages of evolution of cysticercoids in the CNS, lesions may not appear on CT or MRI, and TA detection may contribute to a definite etiologic diagnosis; TA detection may permit diagnosis of NC in some patients with previous negative tests for antibody detection in CSF; TA detection may represent an accurate marker of disease activity in the epileptic form of NC. (author)

  15. INIS, CEA and nuclear terminology; INIS, CEA et terminologie nucleaire

    Energy Technology Data Exchange (ETDEWEB)

    Surmont, J.; Brulet, C.; Constant, A.; Guille, N.; Le Blanc, A.; Mouffron, O.; Anguise, P.; Jouve, J.J

    2007-07-01

    This poster, prepared for the fifth edition of the meetings of scientific and technical information professionals (RPIST, Nancy (France)), presents, first, the INIS information system, its content and coverage, the French participation to this system and the role of the CEA-Saclay as France's official representative for this system. Then it presents the INIS thesaurus with its different levels as a terminological tool for the indexing of documents and for searching documents inside the database. Finally, the very first electronic version of the multilingual thesaurus is introduced. Several national INIS centres, including the CEA-Saclay, have contributed to the translation of lists of new terms and of forbidden terms (synonyms). (J.S.)

  16. The CT Findings and the Peak SUV on PET/CT according to the Levels of Cyfra 21-1 and CEA in Patients with Non-Small Cell Lung Carcinoma

    International Nuclear Information System (INIS)

    We wanted to evaluate the CT findings and the peak SUV according to the levels of cyfra 21-1 and CEA in patients with non-small cell lung carcinoma (NSCLC). We evaluated the TNM staging, cell types, the CT findings and peak SUV of the NSCLC in 234 patients with NSCLC according to the tumor marker levels. The correlations of the CT findings and the peak SUV with the tumor markers were evaluated in 35 patients with stage I disease. The sensitivities of the combined tumor markers cyfra 21-1 and CEA in the NSCLC for each TNM staging (I-IV) were 48.5%, 66.7%, 78.3% and 84.3%, respectively (p<0.05). Cyfra 21-1 was more sensitive for squamous cell carcinoma and CEA was more sensitive for adenocarcinoma. The tumor size, tumor necrosis and peak SUV were greater in the NSCLC with an elevated cyfra 21-1 level than that in the NSCLC without an elevated cyfra 21-1 level (p<0.05). For stage I disease, the level of cyfra 21-1 was linearly correlated with the tumor size (r=0.54) and the peak SUV (r=0.46), and the level of CEA was high in the spiculated masses (p<0.05). The NSCLC with an elevated cyfra 21-1 level shows larger, more frequently necrosis and a higher peak SUV than the NSCLC without an elevated Cyfra 21-1 level. For stage 1 disease, the tumor size and peak SUV correlate with the level of cyfra 21-1, and spiculated masses show an elevated level of CEA

  17. Comparison of a new tumour marker CA 242 with CA 19-9, CA 50 and carcinoembryonic antigen (CEA) in digestive tract diseases.

    OpenAIRE

    Kuusela, P.; Haglund, C.; Roberts, P. J.

    1991-01-01

    The levels of CA 242, a new tumour marker of carbohydrate nature, were measured in sera of 185 patients with malignancies of the digestive tract and of 123 patients with benign digestive tract diseases. High percentages of elevated CA 242 levels (greater than 20 U ml-1) were recorded in patients with pancreatic and biliary cancers (68%). The sensitivity was somewhat lower than that of CA 19-9 (76%) and CA 50 (73%). On the other hand, in benign pancreatic and biliary tract diseases the CA 242 ...

  18. INIS, CEA and nuclear terminology

    International Nuclear Information System (INIS)

    This poster, prepared for the fifth edition of the meetings of scientific and technical information professionals (RPIST, Nancy (France)), presents, first, the INIS information system, its content and coverage, the French participation to this system and the role of the CEA-Saclay as France's official representative for this system. Then it presents the INIS thesaurus with its different levels as a terminological tool for the indexing of documents and for searching documents inside the database. Finally, the very first electronic version of the multilingual thesaurus is introduced. Several national INIS centres, including the CEA-Saclay, have contributed to the translation of lists of new terms and of forbidden terms (synonyms). (J.S.)

  19. Receiver operating characteristic (ROC) curve analysis of the tumour markers CEA, CA 50 and CA 242 in pancreatic cancer; results from a prospective study.

    OpenAIRE

    Pasanen, P. A.; Eskelinen, M.; Partanen, K.; Pikkarainen, P; Penttilä, I.; Alhava, E

    1993-01-01

    The serum values of the tumour markers carcinoembryonic antigen (CEA), cancer-associated carboanhydrate antigens CA 50 and CA 242 were evaluated in 193 patients with hepatopancreato-biliary diseases by receiver operating characteristic (ROC) curve analysis in order to compare their diagnostic accuracy in pancreatic cancer (n = 26), and to define optimal cut-off levels for the serum values of these tumour markers in the diagnosis of pancreatic cancer. The ROC analysis showed that all marker te...

  20. A carcinoembryonic antigen-secreting adenocarcinoma arising in tailgut cyst: clinical implications of carcinoembryonic antigen.

    Science.gov (United States)

    Cho, Byoung Chul; Kim, Nam Kyu; Lim, Beom Jin; Kang, Sang Ook; Sohn, Ju Hyuk; Roh, Jae Kyung; Choi, Sang Tae; Kim, Sung Ai; Park, Se Eun

    2005-08-31

    Tailgut cysts (TGCs) are rare congenital cysts that occur in the retrorectal or presacral spaces. Although most tailgut cysts have been reported as benign, there have been at least 9 cases associated with malignant change. We report herein on an unusual case of a 40-year-old woman with a carcinoembryonic antigen (CEA)-producing adenocarcinoma arising within a TGC who underwent surgical resection and local radiation therapy. Despite the complete resection, metastatic adenocarcinoma developed five months after surgery. CEA-producing adenocarcinoma from a TGC is extremely rare and only two cases, including this case, have been reported in the English medical literature. Besides CEA, the serum levels of CA 19-9 became markedly elevated in this patient. Given that the serum CEA level decreased to the normal range after complete resection of tumor and that the tumor recurrence was associated with a rebound of the CEA serum level, our case shows that serial measurements of serum CEA can be used for treatment planning and for assessing the patient's treatment response for this rare disease. PMID:16127782

  1. Uptake of radiolabeled anti-CEA antibodies in human colorectal primary tumors as a function of tumor mass

    International Nuclear Information System (INIS)

    An inverse correlation has been demonstrated between tumor uptake (u, in units of % injected dose/kg) of monoclonal antibody (Mab) and tumor mass (m, in units of g) for colorectal carcinoma in a series of 19 consecutive patients. The correlation (ρ=-0.510), developed using surgical samples was of the form u=abb and was significant at the 2% level of confidence. All tumors were positive for carcinoembryonic antigen (CEA) and the radiopharmaceutical was in iodine-131 labeled anti-CEA Mab. Such correlations have been predicted earlier from murine and rat tumor uptake data. The slope parameter (b) was -0.362, a number consistent with the previous value (-0.382) found in anti-CEA experiments in mice bearing human xenograft LS174T tumors. (orig.)

  2. The influence of CA 125 and CEA levels on the results of 18F-deoxyglucose positron emission tomography in suspected recurrence of epithelial ovarian cancer

    International Nuclear Information System (INIS)

    Background and Purpose: The follow-up of epithelial ovarian cancer (OCA) consists of clinical investigation, sonography, and tumor markers (TMs), especially CA 125. If tumor recurrence is suspected, other imaging modalities including positron emission tomography (PET) with 18F-deoxyglucose (FDG) are often used. While there is still no consensus about the method of choice and the timing of its application, this study aims to find a TM threshold at which a PET would be appropriate. Material and Methods: A total of 90 PET studies and the associated CA 125 values (normal value <35 U/ml) were available in 71 patients during the follow-up afte primary therapy for OCA. In 48 studies a CEA value (normal value <3 ng/ml) was also available. The results of PET imaging were related to the level of TM increase. Results: In 23/90 studies the PET scan was normal. These patients had a median CA 125 of 13.3 U/ml (range 4.2-168 U/ml). In 67/90 studies the PET indicated a potential recurrence of OCA and the median CA 125 was 166.7 U/ml (range 13.3-4,060 U/ml). The TM levels were significantly different (p<0.001, U-test). With one exception, there were no normal PET scans above CA 125 levels of 30 U/ml; between 20 and 30 U/ml PET was positive in 4/7 studies. Conclusion: In suspected recurrence of OCA, if imaging modalities are to be used, an FDG PET may be considered. Since the costs of this investigation are high, it should be restricted to clinical situations where it is likely to be most effective. In this study a PET indication is worthwhile at CA 125 levels of approximately 30 U/ml. (orig.)

  3. CEA 2005 annual report

    International Nuclear Information System (INIS)

    This document is the 2005 activity report of the French atomic energy commission (CEA). The CEA is a main actor of nuclear research, development and innovation and is involved in three main domains: energy, defense/security, and information/health technologies thanks to high quality research works. With a manpower of 15000 researchers and collaborators with internationally acknowledged competences, the CEA is a driving force of industrial innovation and develops partnerships with French and European industries. It also warrants the perenniality of nuclear dissuasion. This report presents these different aspects of the CEA activities: 1 - defense-security: simulation program, opening to the scientific community, nuclear warheads, nuclear propulsion, cleansing of Rhone valley facilities, permanent monitoring of treaties respect, fight against terrorism; 2 - energy: optimization of the industrial park, advances in long lived radioactive wastes management, future nuclear systems, cleansing and dismantling integration, European nuclear energy research, new energy technologies; 3 - information and health technologies: major challenge of micro- and nano-technologies, key role of software technologies and complex systems; 4 - big research facilities opened to the scientific and industrial communities; 5 - scientific status: scientific evaluation process, prices and honors; 6 - programs support: revisited strategic control, confirmed simplification, active employment and training policy, teaching and training, technological valorization, international relations, communication, continuous quality approach, mastery of facilities safety, security, environmental control, a key-year for information systems. A financial report is attached to the document. (J.S.)

  4. CEA 2009 annual report

    International Nuclear Information System (INIS)

    After an indication of several key figures about the activity of the CEA (Centre d'Etudes Atomiques) and its relationship with the academic as well as the industrial field, in France and worldwide, this 2009 annual report presents its various research programs in the field of defence and of global security: basic research (nuclear weapons and propulsion, struggle against proliferation and terrorism) and applied research (nuclear deterrence, national and international security). Then, it presents the programs in the field of de-carbonated energy: basic research (in material science and in life sciences) and applied research (fission energy, fusion energy, new energy technologies). A last group of research programs deals with information and health technologies and concerns life and material sciences, micro- and nano-technologies, software technologies. Interaction with other research institutions and bodies is also evoked. A brief scientific assessment is proposed. Finally, the different structures building the CEA are presented

  5. CEA - Annual report 2006

    International Nuclear Information System (INIS)

    The CEA, a prominent player in research development and innovation, is active in 3 main areas: energy, health care and information technology and defense and security. This annual report presents the CEA activity for the year 2006 in these three main areas: Science and technology working for nuclear deterrence and global security (the simulation programs, the nuclear warheads, the nuclear propulsion, the decommissioning, the fighting against nuclear proliferation and monitoring international treaties, the global security); health and information technology (micro and nano technologies and systems); energy from nuclear fission and fusion and other technologies that do not emit greenhouse gases (progress for the nuclear industry, sustainable management of radioactive materials and waste, nuclear systems of the future, new energy technologies). (A.L.B.)

  6. CEA and mining industry

    International Nuclear Information System (INIS)

    The French atomic energy commission (CEA) is involved in the mining industry in several ways: - in the front-end of the nuclear industry through its daughter companies and participations in the exploration and exploitation of uranium ores, but also of gold and alloy metals with a 26% participation in Eramet company, the world leader of manganese and nickel. This activity is the main occupation of Cogema daughter company, via the Areva holding; - in the back-end of the nuclear fuel cycle with the delicate problem of the management of radioactive wastes; - in parallel with the nuclear industry through an important activity in semiconductor materials (FCI and SMTElectronics); - and finally through various research works on several mineral compounds. This article focusses on the fuel cycle aspects of the CEA activities and concludes with the research works carried out today on thermonuclear fusion. (J.S.)

  7. A Molecular-Level Account of the Antigenic Hantaviral Surface.

    Science.gov (United States)

    Li, Sai; Rissanen, Ilona; Zeltina, Antra; Hepojoki, Jussi; Raghwani, Jayna; Harlos, Karl; Pybus, Oliver G; Huiskonen, Juha T; Bowden, Thomas A

    2016-05-01

    Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses. PMID:27117403

  8. COLONOSCOPY AND CARCINOEMBRYONIC ANTIGEN VARIATIONS

    Directory of Open Access Journals (Sweden)

    Rita G SOUSA

    2014-03-01

    Full Text Available Context Colonoscopy is essential for synchronous and metachronous cancer detection. Carcinoembryonic antigen is a colorectal cancer tumor marker, important as a follow-up tool in patients with previous colorectal cancer. False-positive carcinoembryonic antigen elevation results in multiples exams and in patient anxiety. In literature, there is reference to transient carcinoembryonic antigen increase with colonoscopy. Objective To evaluate the influence of bowel preparation and colonoscopy in carcinoembryonic antigen blood levels. Methods We prospectively studied subjects that underwent routine colonoscopy in our institution. Blood samples were collected (1 before bowel cleaning, (2 before colonoscopy and (3 immediately after colonoscopy. Blood carcinoembryonic antigen levels were determined by “Sandwich” immunoassay. The statistical methods used were the paired t-test and ANOVA. Results Thirty-seven patients (22M/15F were included; age range 28-84 (mean 56 years. Mean carcinoembryonic antigen values were 1.9, 2 and 1.8 for (1, (2 and (3, respectively. An increase in value (2 compared with (1 was observed in 20/37 patients (P = 0.018, mainly in younger patients and in patients requiring more endoluminal interventions. In 29/37 patients, the CEA value decreased from (2 to (3 (P = 1.3x10-7. Conclusions A trend for carcinoembryonic antigen increase after bowel cleaning was observed, especially in younger patients and in patients with more endoluminal interventions, but without clinical meaning.

  9. Levels of Antibody against 11 Staphylococcus aureus Antigens in a Healthy Population ▿

    OpenAIRE

    Colque-Navarro, Patricia; Jacobsson, Gunnar; Andersson, Rune; Flock, Jan-Ingmar; Möllby, Roland

    2010-01-01

    Serum samples from 151 healthy individuals aged from 15 to 89 years were investigated by enzyme-linked immunosorbent assay (ELISA) for IgG levels against 11 different purified antigens from Staphylococcus aureus. Surface antigens, such as teichoic acid, clumping factors A and B, and bone sialoprotein binding protein, and extracellular proteins, such as alpha-toxin, lipase, enterotoxin A, toxic shock syndrome toxin, scalded-skin syndrome toxin, fibrinogen binding protein, and extracellular adh...

  10. Antibody to Hepatitis B Core Antigen Levels in the Natural History of Chronic Hepatitis B

    OpenAIRE

    Jia, Wei; Song, Liu-Wei; Fang, Yu-Qing; Xiao-feng WU; Liu, Dan-Yang; Xu, Chun; Wang, Xiao-Mei; Wang, Wen; Lv, Dong-Xia; Li, Jun; Deng, Yong-Qiong; Wang, Yan; Huo, Na; Yu, Min; Xi, Hong-Li

    2014-01-01

    Abstract Previous studies have revealed antibody to hepatitis B core antigen (anti-HBc) levels as a predictor of treatment response in hepatitis B early antigen (HBeAg)-positive chronic hepatitis B (CHB) patients in both interferon and nucleos(t)ide analog therapy cohorts. However, there is no information about anti-HBc levels in the natural history of CHB. This study aimed to define anti-HBc levels of different phases in the natural history of CHB. Two hundred eleven treatment-naive CHB pati...

  11. Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Wang Zhi-qiang

    2010-10-01

    Full Text Available Abstract Background The existence of circulating tumor cells (CTCs in peripheral blood as an indicator of tumor recurrence has not been clearly established, particularly for gastric cancer patients. We conducted a retrospective analysis of the relationship between CTCs in peripheral blood at initial diagnosis and clinicopathologic findings in patients with gastric carcinoma. Methods Blood samples were obtained from 123 gastric carcinoma patients at initial diagnosis. mRNA was extracted and amplified for carcinoembryonic antigen (CEA mRNA detection using real-time RT-PCR. Periodic 3-month follow-up examinations included serum CEA measurements and imaging. Results The minimum threshold for corrected CEA mRNA score [(CEA mRNA/GAPDH mRNA × 106] was set at 100. Forty-five of 123 patients (36.6% were positive for CEA mRNA expression. CEA mRNA expression significantly correlated with T stage and postoperative recurrence status (P = 0.001. Recurrent disease was found in 44 of 123 cases (35.8%, and 25 of these (56.8% were positive for CEA mRNA. Of these patients, CEA mRNA was more sensitive than serum CEA in indicating recurrence. Three-year disease-free survival of patients positive for CEA mRNA was significantly poorer than of patients negative for CEA mRNA (P Conclusions CEA mRNA copy number in peripheral blood at initial diagnosis was significantly associated with disease recurrence in gastric adenocarcinoma patients. Real-time RT-PCR detection of CEA mRNA levels at initial diagnosis appears to be a promising predictor for disease recurrence in gastric adenocarcinoma patients.

  12. Concomitant hepatic radiation and intraarterial fluorinated pyrimidine therapy: correlation of liver scan, liver function tests, and plasma CEA with tumor response

    International Nuclear Information System (INIS)

    Sixteen patients with metastatic disease to the liver (12 colorectal and four unknown primary tumors) were treated in a pilot study of hepatic irradiation (2500-3000 rads in 10-12 fractions) delivered concomitantly with continuous short-term intraarterial infusion of 5-fluorouracil (1 g/d) or FUDR (0.5 mg/kg/d) via a percutaneously placed hepatic artery catheter. Abnormal liver function tests, including SGOT, LDH, and alkaline phosphatase, decreased in all patients by day 7-10 of treatment, and other metabolic factors, including serum cholesterol, calcium, albumin, phosphorous, and uric acid, also decreased, often to subnormal levels by termination of treatment (day 15-20). These chemical alterations did not correlate with tumor response in that the identical pattern was observed in responders (ten patients) as well as nonresponders (six patients). Objective determinants of response were assessed by serial monitoring of the plasma carcinoembryonic antigen (CEA) and liver scan. In 14 patients with elevated CEA levels, tumor response (nine patients), nonresponse (four patients), and relapse (five patients) was predicted and confirmed by sequential monitoring of CEA. In one patient, a paradoxical decrease in plasma CEA was associated with progressive disease. The liver scan identified all responding patients but was difficult to quantitate and was delayed for months following subjective clinical response and changes in plasma CEA levels

  13. Serum Prostatic Specific Antigen Levels in Women with Hirsutism

    Directory of Open Access Journals (Sweden)

    Filiz Cebeci

    2009-09-01

    Full Text Available Background and Design: Using an ultrasensitive assay, PSA has been detected in female serum and has been proposed as a potential marker of androgen excess in hirsute women. The object of this present study was to investigate an association between hirsutism and serum PSA levels.Material and Method: A total of 47 women with hirsutism and 24 age-matched nonhirsute women were included in this study. Using an ultrasensitive chemiluminescent immunoassay serum PSA were determined in all subjects.Results: Serum levels of PSA were higher in hirsute women than in nonhirsute controls (p<0.05.Conclusion: If more sensitive assays become available, PSA might be used as biochemical marker of peripheral androgen excess in women with hirsutism.

  14. Cell density related gene expression: SV40 large T antigen levels in immortalized astrocyte lines

    Directory of Open Access Journals (Sweden)

    Jacobberger James W

    2002-04-01

    Full Text Available Abstract Background Gene expression is affected by population density. Cell density is a potent negative regulator of cell cycle time during exponential growth. Here, we asked whether SV40 large T antigen (Tag levels, driven by two different promoters, changed in a predictable and regular manner during exponential growth in clonal astrocyte cell lines, immortalized and dependent on Tag. Results Expression and cell cycle phase fractions were measured and correlated using flow cytometry. T antigen levels did not change or increased during exponential growth as a function of the G1 fraction and increasing cell density when Tag was transcribed from the Moloney Murine Leukemia virus (MoMuLV long terminal repeat (LTR. When an Rb-binding mutant T antigen transcribed from the LTR was tested, levels decreased. When transcribed from the herpes thymidine kinase promoter, Tag levels decreased. The directions of change and the rates of change in Tag expression were unrelated to the average T antigen levels (i.e., the expression potential. Conclusions These data show that Tag expression potential in these lines varies depending on the vector and clonal variation, but that the observed level depends on cell density and cell cycle transit time. The hypothetical terms, expression at zero cell density and expression at minimum G1 phase fraction, were introduced to simplify measures of expression potential.

  15. Combination of cancer antigen 125 and carcinoembryonic antigen can improve ovarian cancer diagnosis

    DEFF Research Database (Denmark)

    Sørensen, Sofie Sølvsten; Mosgaard, Berit Jul

    2011-01-01

    The purpose of the present study was to evaluate the ability of the tumour marker carcinoembryonic antigen (CEA) in combination with cancer antigen 125 (CA-125) to differentiate between malignant ovarian and malignant non-ovarian disease.......The purpose of the present study was to evaluate the ability of the tumour marker carcinoembryonic antigen (CEA) in combination with cancer antigen 125 (CA-125) to differentiate between malignant ovarian and malignant non-ovarian disease....

  16. CEA sustainable development report 2007; CEA rapport developpement durable 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The CEA, a prominent player in research development and innovation, is active in three main domains: energy, health care and information technology, defense and security. This annual report presents the CEA activities in the domain of the sustainable development. The first part is devoted to the environment preservation policy (energy, water, air, chemistry, wastes, transport, buildings). The second part shows the dynamic governance in the domain of the risks management. The last part presents the CEA activities of research for the sustainable development. (A.L.B.)

  17. CEA Annual report 2007; CEA rapport annuel 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The CEA, a prominent player in research development and innovation, is active in three main domains: energy, health care and information technology, defense and security. This annual report presents the CEA activities for the year 2007 in these three main areas: science and technology working for nuclear deterrence and global security, the energies without greenhouse effect gases emission against the climatic change, researches in the information sciences and technologies for a better communication and health. The CEA safety, organization, communication and international relations are also presented. (A.L.B.)

  18. CEA financial report 2007; CEA rapport financier 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This document provides financial data on the CEA for the year 2007. The management report (budget, resources, expenditures) and the accounting are detailed. The main management events of the year 2007 are presented. (A.L.B.)

  19. CEA - 2014 risk management assessment

    International Nuclear Information System (INIS)

    After introducing presentations of CEA managers in charge of risk management and controls, this document presents and comments the actions undertaken by the CEA and the obtained results in terms of risk management in different fields: protection and control of the environment, installation safety, health, safety and radiation protection, transport of hazardous materials, waste management, protection of sites, installations and heritage, management of emergency situations, management of legal risks, internal audits and controls. Other topics are addressed like the presentation of the risk management department, and the role of the CEA in the relationship between research and industry

  20. CEA sustainable development report 2007

    International Nuclear Information System (INIS)

    The CEA, a prominent player in research development and innovation, is active in three main domains: energy, health care and information technology, defense and security. This annual report presents the CEA activities in the domain of the sustainable development. The first part is devoted to the environment preservation policy (energy, water, air, chemistry, wastes, transport, buildings). The second part shows the dynamic governance in the domain of the risks management. The last part presents the CEA activities of research for the sustainable development. (A.L.B.)

  1. The organisation of criticality hazard prevention at the CEA

    International Nuclear Information System (INIS)

    This paper deals with the organisation of criticality hazard prevention at the French Atomic Energy Commission (CEA). This new organization has been experimented since the end of year 2000. During the first semester of year 2002, the CEA nuclear inspection team, who is in charge of the control function at the CEA general administration level, has performed an inquiry to check the effectiveness of the new organization. The conclusions of this inquiry are very positive; a few recommendations are now taken in to account to further improve the efficiency of this organization. (J.P.N.)

  2. Prostate-Specific Antigen Velocity Before and After Elimination of Factors That Can Confound the Prostate-Specific Antigen Level

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jessica J., E-mail: park16@fas.harvard.edu [Department of Radiation Oncology, Brigham and Women' s Hospital and Dana-Farber Cancer Institute, Boston, MA (United States); Chen, Ming-Hui [Department of Statistics, University of Connecticut, Storrs, CT (United States); Loffredo, Marian; D' Amico, Anthony V. [Department of Radiation Oncology, Brigham and Women' s Hospital and Dana-Farber Cancer Institute, Boston, MA (United States)

    2012-03-01

    Purpose: Prostate-specific antigen (PSA) velocity, like PSA level, can be confounded. In this study, we estimated the impact that confounding factors could have on correctly identifying a patient with a PSA velocity >2 ng/ml/y. Methods and Materials: Between 2006 and 2010, a total of 50 men with newly diagnosed PC comprised the study cohort. We calculated and compared the false-positive and false-negative PSA velocity >2 ng/ml/y rates for all men and those with low-risk disease using two approaches to calculate PSA velocity. First, we used PSA values obtained within 18 months of diagnosis; second, we used values within 18 months of diagnosis, substituting the prebiopsy PSA for a repeat, nonconfounded PSA that was obtained using the same assay and without confounders. Results: Using PSA levels pre-biopsy, 46% of all men had a PSA velocity >2 ng/ml/y; whereas this value declined to 32% when substituting the last prebiopsy PSA for a repeat, nonconfounded PSA using the same assay and without confounders. The false-positive rate for PSA velocity >2 ng/ml/y was 43% as compared with a false-negative rate of PSA velocity >2 ng/ml/y of 11% (p = 0.0008) in the overall cohort. These respective values in the low-risk subgroup were 60% and 16.7% (p = 0.09). Conclusion: This study provides evidence to explain the discordance in cancer-specific outcomes among groups investigating the prognostic significance of PSA velocity >2 ng/ml/y, and highlights the importance of patient education on potential confounders of the PSA test before obtaining PSA levels.

  3. Prognostic value of monitoring tumour markers CA 15-3 and CEA during fulvestrant treatment

    Directory of Open Access Journals (Sweden)

    Locker Gottfried J

    2006-03-01

    Full Text Available Abstract Background At many centres tumour markers are used to detect disease recurrence and to monitor response to therapy in patients with advanced disease, although the real value of serial observation of marker levels remains disputed. In this study, we evaluated the prognostic value of tumour markers for predicting response (partial response [PR], stable disease [SD] ≥ 6 months, de novo disease progression (PD and secondary PD in patients receiving fulvestrant ('Faslodex' 250 mg/month for the treatment of metastatic breast cancer (MBC. Methods Changes in cancer antigen 15–3 (CA 15-3 and carcinoembryonic antigen (CEA were prospectively monitored (monthly and were also evaluated for the 3 months preceding secondary PD. Data from 67 patients with previously treated MBC participating in a Compassionate Use Programme were analysed. Results In patients with a PR (n = 7 [10.4%], a non-significant increase in CA 15-3 occurred during the first 6 months of treatment; CEA was significantly reduced (P = 0.0165. In patients with SD ≥ 6 months (n = 28 [41.8%], both CA 15-3 (P P = 0.0399 levels increased significantly after 6 months treatment. In those experiencing de novo PD (n = 32 [47.8%], CA 15-3 increased significantly (P P = 0.0002 during the same time period. Both CA 15-3 (P P Conclusion CA 15-3 increases in patients progressing on fulvestrant but may also increase in those experiencing clinical benefit; this should not be taken as a sign of PD without verification. Overall, both CA 15-3 and CEA appear to be poor prognostic markers for determining progression in patients receiving fulvestrant.

  4. Serum tumor marker CYFRA 21-1 in the diagnostics of squamous cell lung cancer - comparison with CEA

    International Nuclear Information System (INIS)

    The aim of the study was to test the diagnostic value tumor marker CYFRA 21-1 for squamous cell lung cancer (SQCLC) in comparison with carcinoembryonic antigen (CEA). Ninety-one patients were induced in this study: 56 with SQCLC - Group I, 25 with other types of lung cancer - Group II, 10 with benign respiratory tract diseases - Group III. Median CYFRA 21-1 serum concentration (ng/ml) was: in Group I: 4.52 (0.94 - >16), in Group II: 3.58 (1.72 - >16), in Group III: 2.05 (0.99 - 3.41). Median CEA serum concentration (ng/ml) was: in Group I: 4.49 (076- >20), in Group II: 3.32 (1.17 - >20), in Group III: 3.09 (1.84 - 6.37). There was a highly significant difference between the levels of CYFRA 21-1 in group I and Group III (p < 0.001), but there was no statistically significant difference between the levels of CEA in Group I and III. Sensitivity of CYFRA 21-1 by the cut-off 3.33 ng/ml in the diagnostics of SQCLC was 0.68, specificity 0.090, positive predictive value 0.91, negative predictive value 0.65. Sensitivity of CEA by cut-off 4.61 ng/ml was 0.5 by the same specificity 0.90. CYFRA 21-1 has high sensitivity, specificity and positive predictive value in the diagnostics of SQCLC. Sensitivity of CYFRA 21-1 is significantly higher than sensitivity of CEA in this settings. (author)

  5. Ecotoxicological monitoring at CEA - Centro Experimental Aramar

    International Nuclear Information System (INIS)

    The environmental preservation has been one of the major targets of the Centro Experimental Aramar (CEA), since its foundation. The research center is located near Ipero, SP, and is maintained by Centro Tecnologico da Marinha em Sao Paulo - CTMSP. An Environmental Monitoring Program is developed with many types of matrices being periodically collected and analysed, including all the gaseous and liquids emissions liberated by the facilities. Besides radiometric and chemical analysis we have been testing bioassays for the effluents to accomplish the new recommendations from governmental agencies. The microcrustacean Daphnia similis has been tested in toxicity assays on liquid effluents and the garden plant Tradescantia pallida has been used to assess the genotoxicity effects from gaseous emissions. The results show that liquid and gaseous emissions from CEA are bellow the regulatory levels. (author)

  6. Relationship between peripheral and mesenteric serum levels of CEA and CA 242 with staging and histopathological variables in colorectal adenocarcinoma Níveis séricos periféricos e mesentéricos de CEA e CA 242, estadiamento e variáveis histopatológicas no adenocarcinoma colorretal

    OpenAIRE

    Mauro Lamelas Cardoso; Luís Cesar Fernandes; Su Bong Kim; Delcio Matos

    2009-01-01

    PURPOSE: To compare histopathological variables and staging in colorectal adenocarcinoma cases with CEA and CA 242 in peripheral and mesenteric blood. METHODS: In 169 individuals underwent surgery for colorectal cancer, CEA and CA 242 were analyzed and compared to mesenteric and peripheral blood and correlated with macroscopic tumor's morphology and size, degree of cell differentiation, venous, neural and lymphatic involvement and TNM classification. RESULTS: There was a difference between th...

  7. Development of a double-antibody radioimmunoassay for detecting ovarian tumor-associated antigen fraction OCA in plasma

    International Nuclear Information System (INIS)

    Ovarian tumor-associated antigen isolated from human tumor tissue was shown to have a different mobility from that of carcinoembryonic antigen (CEA) in both acrylamide gel electrophoresis and immunoelectrophoresis in agarose. The ovarian tumor antigen is composed of six species with different electrophoretic mobility in acrylamide gel electrophoresis. Three of these species were detected in Sephadex G-100 ovarian fraction OCA (from the void volume peak) and the other three species of lower apparent molecular weight were detected in fraction OCD (from the second peak). Fractions OCA and OCD did not share common antigenic determinants as determined by immunodiffusion. CEA was shown to share antigenic determinants with both OCA and OCD. A double-antibody radioimmunoassay capable of detecting nanogram quantities of plasma OCA was developed. In a preliminary study of ovarian cancer patients, OCA appeared to be a more sensitive marker for ovarian cancer than CEA. There was virtually no correlation (r2 = 0.1) between OCA and CEA levels in these patients, as determined by radioimmunoassay

  8. Scientific evaluation at the CEA; Evaluation scientifique au CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-11-01

    This report presents a statement of the scientific and technical activity of the French atomic energy commission (CEA) for the year 1998. This evaluation is made by external and independent experts and requires some specific dispositions for the nuclear protection and safety institute (IPSN) and for the direction of military applications (DAM). The report is divided into 5 parts dealing successively with: part 1 - the CEA, a public research organization (civil nuclear research, technology research and transfers, defence activities); the scientific and technical evaluation at the CEA (general framework, evaluation of the IPSN and DAM); part 2 - the scientific and technical councils (directions of fuel cycle, of nuclear reactors, and of advanced technologies); part 3 - the scientific councils (directions of matter and of life sciences); the nuclear protection and safety institute; the direction of military applications; part 4 - the corresponding members of the evaluation; part 5 - the list of scientific and technical councils and members. (J.S.)

  9. Combined effects of 5-Fluorouracil, Folinic acid and Oxaliplatin on the expression of carcinoembryonic antigen in human colon cancer cells: pharmacological basis to develop an active antitumor immunochemotherapy

    Directory of Open Access Journals (Sweden)

    De Vecchis Liana

    2008-05-01

    Full Text Available Abstract Background Five-fluorouracil (FU, mainly associated with leucovorin (L, plays an essential role in chemotherapy of colorectal carcinoma. Moreover, FU ± L has been found to increase the expression of tumor-associated carcinoembryonic antigen (CEA, that may be an important target in therapeutic protocols of active specific immunotherapy. FU + L (FUL are frequently combined with oxaliplatin (OXA in advanced colon cancer patients. Thus, we investigated whether FUL in combination with OXA according to 2 different schedules may influence CEA expression in human colon cancer cells in vitro. Methods CEA protein expression was evaluated by cytofluorimetric and western blot analysis. Relative quantification of CEA mRNA was assessed by real time RT-PCR analysis. Results Levels of CEA protein and transcript were found to be higher in FUL-treated cells than in controls. However, when target cells were exposed to OXA before but not after FUL treatment, the up-regulation of CEA was partially inhibited. Conclusion These results suggest that target cells must be exposed to OXA after but not before treatment with the fluoropyrimidine in order to exploit drug-induced up-regulation of CEA. This finding appears to provide useful information to design chemo-immunotherapy protocols based on FUL + OXA, combined with host's immunity against CEA directed cancer vaccines.

  10. Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma

    Science.gov (United States)

    Yuan, Chuanshu; Yang, Kai; Tang, Hong; Chen, Dan

    2016-01-01

    Background At present, the research on serum tumor markers in the early diagnosis of malignant tumors has aroused widespread concern. The aim of this study was to investigate the diagnostic values of serum tumor markers cytokeratin 19 fragment (Cyfra21-1), squamous cell carcinoma antigen (SCCAg), ferritin, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and α-fetoprotein (AFP) for patients with oral/oropharyngeal squamous carcinoma (OSCC/OPSCC). Methods One hundred and sixty-nine cases of patients with OSCC/OPSCC as the experimental group, 86 cases of oral benign tumor patients as the control group, and 30 cases of healthy people as the normal control group were studied. The levels of serum Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP were measured using electrochemiluminescence immunoassay. Results The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in patients with OSCC/OPSCC were significantly higher than those of benign tumor and healthy control group (P0.05). The level of serum Cyfra21-1 in patients with early OSCC/OPSCC (stage I + II) was significantly higher than that of benign tumor and healthy control group (P0.05). The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in the middle-late stage of patients with OSCC/OPSCC (stage III + IV) were significantly higher than those of patients with the early OSCC/OPSCC, benign tumor, and healthy control group (P<0.05). The diagnostic cutoff levels of Cyfra21-1, SCCAg, ferritin, and CEA were 2.17, 0.72, 109.95, and 1.99 ng/mL, respectively. The sensitivities were 60.36%, 73.37%, 81.66%, and 66.27%, respectively. The specificities were 81.03%, 68.10%, 40.52%, and 61.21%, respectively. Conclusion Cyfra21-1, SCCAg, ferritin, and CEA had diagnostic values for patients with OSCC/OPSCC. Meanwhile, Cyfra21-1 had better early diagnostic value for patients with OSCC/OPSCC. PMID:27350753

  11. CEA Annual progress report 1986

    International Nuclear Information System (INIS)

    This annual report presents the general organization of the CEA, the international relations and politics in nuclear field, the activities (military application, nuclear applied research, ANDRA (National Agency for Radioactive Waste Management), nuclear safety and protection, fundamental research, applied research other than nuclear), the industrial group; among topics about men and means, the budget execution of the public establishment of research. In annex, the nuclear power plants around the world and the principal legislative texts related to CEA or atomic energy published in 1986

  12. CEA 2005 annual report; CEA rapport annuel 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This document is the 2005 activity report of the French atomic energy commission (CEA). The CEA is a main actor of nuclear research, development and innovation and is involved in three main domains: energy, defense/security, and information/health technologies thanks to high quality research works. With a manpower of 15000 researchers and collaborators with internationally acknowledged competences, the CEA is a driving force of industrial innovation and develops partnerships with French and European industries. It also warrants the perenniality of nuclear dissuasion. This report presents these different aspects of the CEA activities: 1 - defense-security: simulation program, opening to the scientific community, nuclear warheads, nuclear propulsion, cleansing of Rhone valley facilities, permanent monitoring of treaties respect, fight against terrorism; 2 - energy: optimization of the industrial park, advances in long lived radioactive wastes management, future nuclear systems, cleansing and dismantling integration, European nuclear energy research, new energy technologies; 3 - information and health technologies: major challenge of micro- and nano-technologies, key role of software technologies and complex systems; 4 - big research facilities opened to the scientific and industrial communities; 5 - scientific status: scientific evaluation process, prices and honors; 6 - programs support: revisited strategic control, confirmed simplification, active employment and training policy, teaching and training, technological valorization, international relations, communication, continuous quality approach, mastery of facilities safety, security, environmental control, a key-year for information systems. A financial report is attached to the document. (J.S.)

  13. Perioperative prostate specific antigen levels among coronary artery bypass grafting patients: Does extracorporeal circulation and body temperature induce prostate specific antigen levels alterations?

    Science.gov (United States)

    Patris, Emmanuel; Giakoumidakis, Konstantinos; Patris, Vasileios; Kuduvalli, Manoj; Argiriou, Mihalis; Charitos, Christos; Kalaitzis, Christos; Touloupidis, Stavros

    2015-01-01

    Purpose: The purpose of this study is to compare the perioperative total prostate specific antigen (tPSA) levels among coronary artery bypass grafting (CABG) patients with and without extracorporeal circulation (ECC), to investigate the changes overtime of tPSA in each group separately and to determine the effect of body core temperature on tPSA levels. Materials and Methods: A prospective study was conducted. Our sample was allocated to: (a) Seven patients who underwent off pump CABG (Group I) and (b) 16 CABG patients with ECC (Group II). The levels of tPSA were measured preoperatively (baseline), intra-operatively and at the 4th postoperative day. We compared the two groups on their tPSA levels and we investigated the changes of tPSA overtime in each group separately. Results: Intra-operative serum samples were obtained in significantly lower body temperature in patients of Group II than in those of Group I (31°C vs. 36.9°C, P < 0.001). In each group separately, postoperative tPSA levels were increased significantly compared to the baseline values (2.55 ng/ml vs. 0.39 ng/ml for Group I, P = 0.005 and 4.36 ng/ml vs. 0.77 for Group II, P < 0.001). CABG patients with ECC had significantly lower intra-operative tPSA levels than the baseline values (0.67 ng/ml vs. 0.77 ng/ml, P = 0.008). We did not observe significant differences of tPSA levels between the two groups. Conclusions: CABG surgery affects similarly the perioperative tPSA independently the involvement of ECC. Although all patients had significantly higher early postoperative tPSA levels, only those who underwent CABG with ECC had exceeded normal values and significantly decreased intra-operative tPSA. Hypothermia seems to be the causal factor of tPSA reduction. PMID:25657546

  14. Normal carcinoembryonic antigen indicates benefit from perioperative chemotherapy to gastric carcinoma patients

    Institute of Scientific and Technical Information of China (English)

    Shi Chen; Ying-Bo Chen; Yuan-Fang Li; Xing-Yu Feng; Zhi-Wei Zhou; Xiu-Hong Yuan; Chao-Nan Qian

    2012-01-01

    AIM:To evaluate pretreatment serum carcinoembryonic antigen (CEA) as a predictor of survival for patients with locally advanced gastric cancer receiving perioperative chemotherapy.METHODS:We retrospectively studied a cohort of 228gastric cancer patients who underwent D2 gastrectomy combined with chemotherapy at the Sun Yat-sen University Cancer Center between January 2005 and December 2009.Among them,168 patients received 6-12 cycles of oxaliplatin-based adjuvant (post-operative) chemotherapy,while 60 received perioperative chemotherapy (2 cycles of FOLFOX6 or XELOX before surgery and 4-10 cycles after surgery).Serum CEA was measured using an enzyme immunoassay.The followup lasted until December 2010.RESULTS:In the group that had elevated serum CEA,the difference in survival time between patients receiving perioperative chemotherapy and those receiving adjuvant chemotherapy had no statistical significance (P >0.05).However,in the group that had normal serum CEA,patients receiving perioperative chemotherapy had a longer survival time.In multivariate analysis,T staging and lymph node metastatic rate were independent prognostic factors for the patients.Perioperative chemotherapy improved the overall survival of patients who had a normal pretreatment CEA level (P =0.070).CONCLUSION:Normal pretreatment serum CEA is a predictor of survival for patients receiving perioperative chemotherapy.

  15. CEA - Annual report 2006; CEA - Rapport annuel 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    The CEA, a prominent player in research development and innovation, is active in 3 main areas: energy, health care and information technology and defense and security. This annual report presents the CEA activity for the year 2006 in these three main areas: Science and technology working for nuclear deterrence and global security (the simulation programs, the nuclear warheads, the nuclear propulsion, the decommissioning, the fighting against nuclear proliferation and monitoring international treaties, the global security); health and information technology (micro and nano technologies and systems); energy from nuclear fission and fusion and other technologies that do not emit greenhouse gases (progress for the nuclear industry, sustainable management of radioactive materials and waste, nuclear systems of the future, new energy technologies). (A.L.B.)

  16. Carcinoembryonic antigen-producing adrenal adenoma resected using combined lateral and anterior transperitoneal laparoscopic surgery

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A 74-year-old woman presented with symptoms consistent with hyperadrenocorticism and hyperca techolaminism. She had a cushingoid appearance and her cortisol level was elevated. Herserum dopamine and noradrenalin levels were also elevated.Computed tomography detected a left adrenal mass measuring 3.5 cm × 3.0 cm in diameter. Metaiodobenzylguanidine cintigraphy was negative. Unexpectedly, the serum Serum carcinoembryonic antigen (CEA) level was elevated.Fluorodeoxyglucose positron emission tomography showed increased uptake in the adrenal tumor only, with a maximum standardized uptake value of 2.8. Selective venography and blood sampling revealed that the concentrations of cortisol, catecholamines and CEA were significantly elevated in the vein draining the tumor. A diagnosis of CEA-produdng benign adenoma was made. After preoperative management, we performed a combined lateral and anterior transperitoneal laparoscopic adrenectomy. Her vital signs remained stable during surgery. Histopathological examination revealed a benign adenoma. Her cortisol, catecholamine and CEA levels normalized immediately after surgery. We present, to the best of our knowledge, the first case of CEA-roducin gadrenal adenoma, along with a review of the relevant literature, and discuss our laparoscopic surgery techniques.

  17. Association between high serum carcinoembryonic antigen and clinical state of male anorexia nervosa: A case report.

    Science.gov (United States)

    Ochi, Shinichiro; Shimizu, Hideaki; Ueno, Shu-Ichi

    2016-04-01

    Anorexia nervosa (AN) is a complex psychiatric disorder, which is not yet fully understood. Several studies reported that AN was associated with disruption of cytokine network. Carcinoembryonic antigen (CEA) is a glycoprotein related to its network, used as a tumor marker of adenocarcinoma, and suggested to stimulate monocytes and macrophages to release proinflammatory cytokines. Here, we report a 41-year-old male suffering from AN who was suspected of having a malignant tumor due to markedly elevated serum CEA levels. However, on further examinations, he was discovered to have no malignant tumors, and, interestingly, his CEA levels actually decreased as his clinical state of AN improved. Furthermore, it was found that his CEA levels were elevated proportionally to his clinical state of AN and that his body mass index was significantly correlated with serum CEA levels. Therefore, it is suggested that inflammatory responses may be associated with the clinical state of AN. © 2016 Wiley Periodicals, Inc. (Int J Eat Disord 2016; 49:428-431). PMID:27015461

  18. Increased basolateral sorting of carcinoembryonic antigen in a polarized colon carcinoma cell line after cholesterol depletion-Implications for treatment of inflammatory bowel disease

    Institute of Scientific and Technical Information of China (English)

    Robert Ehehalt; Markus Krautter; Martin Zorn; Richard Sparla; Joachim Fūllekrug; Hasan Kulaksiz; Wolfgang Stremmel

    2008-01-01

    AIM:To investigate a possible increase of basolateral expression of carcinoembryonic antigen(CEA)by interfering with the apical transport machinery,we studied the effect of cholesterol depletion on CEA sorting and secretion.METHODS:Cholesterol depletion was performed in polarized Caco-2 cells using Iovastatin and methyl-βcyclodextrin.RESULTS:We show that CEA is predominantly expressed and secreted at the apical surface.Reduction of the cholesterol level of the cell by 40%-50% with Iovastatin and methyl-β-cyclodextrin led to a significant change of the apical-to-basolateral transport ratio towards the basolateral membrane.CONCLUSION:As basolateral expression of CEA has been suggested to have anti-inflamatory properties,Cholesterol depletion of enterocytes might be a potential approach to influence the course of inflammatory bowel disease.

  19. Ecotoxicological monitoring at CEA - Centro Experimental Aramar; Monitoramento ecotoxicologico no CEA - Centro Experimental Aramar

    Energy Technology Data Exchange (ETDEWEB)

    Mattiolo, Sandra Regina; Machado, Alessandra Carla Fattori Ergesse [Centro Tecnologico da Marinha em Sao Paulo (CTMSP), Sao Paulo, SP (Brazil)]. E-mail: sandra@ctmsp.mar.mil.br; alessan@ctmsp.mar.mil.br

    2005-07-01

    The environmental preservation has been one of the major targets of the Centro Experimental Aramar (CEA), since its foundation. The research center is located near Ipero, SP, and is maintained by Centro Tecnologico da Marinha em Sao Paulo - CTMSP. An Environmental Monitoring Program is developed with many types of matrices being periodically collected and analysed, including all the gaseous and liquids emissions liberated by the facilities. Besides radiometric and chemical analysis we have been testing bioassays for the effluents to accomplish the new recommendations from governmental agencies. The microcrustacean Daphnia similis has been tested in toxicity assays on liquid effluents and the garden plant Tradescantia pallida has been used to assess the genotoxicity effects from gaseous emissions. The results show that liquid and gaseous emissions from CEA are bellow the regulatory levels. (author)

  20. CEA: risk management assessment 2011

    International Nuclear Information System (INIS)

    This report proposes a qualitative and quantitative overview of CEA activities in the field of risk management during 2011. These activities concerned the impact on the environment, the safety of installations, the management of professional risks (safety and health at work), the radiological protection of workers, the transports of hazardous materials, waste management, protection of sites, installations and heritage, the management of emergency situations, the management of law risks, controls and audits

  1. Advanced prostatic carcinomas with low serum levels of prostate-specific antigen

    Directory of Open Access Journals (Sweden)

    Cerović Snežana J.

    2002-01-01

    Full Text Available The serum levels of prostate-specific antigen (PSA represent a significant diagnostic and monitoring parameter of prostatic carcinoma (PC. The aim of the study was to establish correlation of serum PSA level in addition to grade, histological type, and clinical stage of PC in patients with normal or intermediary PSA serum level. In 37 untreated PC patients with preoperative serum PSA levels ranging between 0.1 and 9.6 ng/ml, paraffin-embedded tissue and serum samples were immunohistological studied and immunoassay for PSA was done. The most representative was poorly differentiated PC with D stage In serum samples from PC patients 27 (73.7% normal (≤ 4.0 ng/ml, and 10 (27.3% intermediate (4.1-10 ng/ml PSA levels were found Immunohistochemistry, in 36 PC (97.3% had demonstrated the expression of PSA. Our study results had shown low serum PSA levels in some patients with advanced poorly differentiated PC.

  2. Selectively assaying CEA based on a creative strategy of gold nanoparticles enhancing silver nanoclusters' fluorescence.

    Science.gov (United States)

    Yang, Xiaoming; Zhuo, Yan; Zhu, Shanshan; Luo, Yawen; Feng, Yuanjiao; Xu, Yan

    2015-02-15

    Herein, we have successfully built up connections between nanoparticles and nanoclusters, and further constructed a surface-enhanced fluorescence (SEF) strategy based on the two types of nanomaterials for selectively assaying carcinoembryonic antigen (CEA). Specifically, silver nanoclusters provided the original fluorescence signal, while gold nanoparticles modified with DNA served as the fluorescence enhancer simultaneously. On the basis of this proposed nano-system, the two nanomaterials were linked by CEA-aptamer, thus facilitating SEF occurring. Nevertheless, more competitive interactions between CEA and CEA-aptamer emerged once CEA added, leading to SEF failed and their fluorescence decreased. Significantly, this creative method was further applied to detect CEA, and showed the linear relationship between the fluorescence intensity and CEA concentrations in the range of 0.01-1 ng mL(-1) with a detection limit of 3 pg mL(-1) at a signal-to-noise ratio of 3, demonstrating its sensitivity and promising towards multiple applications. On the whole, this approach we established may broaden potential ways of combining nanoparticles and nanoclusters for detecting trace targets in bioanalytical fields. PMID:25259877

  3. The Effects of PPI Use on Clostridium Difficile Serum Antigen Levels

    Directory of Open Access Journals (Sweden)

    Güçlü Beriat

    2012-09-01

    Full Text Available Aim To evaluate the effects of proton-pump inhibitor (PPI use on distrubtion of intestinal flora  by measuring serum Clostridium Difficile antigen levels before and at the end of a three-months treatment  in patients with Laryngopharyngeal reflux (LFR treatment . Material and Methods The study covers 32 patients with LFR, out of which 24 were female (75% and 8 were male (25%. The mean age of the patients was 34.13 ± 11.59. All patients included in the study were administered Lansoprazole 30 mgr tablets perorally before meals and twice a day for treatment. Reflux Symptom Index (RSI, Reflux Finding Score (RFS, white blood cell count, CRP and serum Clostridium Difficile toxin A, B measurement results were comparatively evaluated through the blood serum samples drawn from the patients before and at the end of the three-months treatment. Results While the mean values of pre-treatment RSI and RFI were 20.81±4.05 and 13.31±3.30 respectively, the mean values were measured to be 3.41±2.37 and 1.50±1.88 respectively following the three-months treatment (p< 0.05. The pre-treatment mean value of serum Clostridium Difficile Ag was 140.56±11.74, while it was seen that the same value became 114.94±10.70 after the three-months treatment (p< 0.05. There was, however, no statistically significant change in the other parameters. Discussion According to the results obtained, it was seen that the treatment with PPI was not cause to  increase Clostridium difficile toxin A, B serum antigen levels. So these drugs could be used in long time therapies confidently. 

  4. Tumor marker utility and prognostic relevance of cathepsin B, cathepsin L, urokinase-type plasminogen activator, plasminogen activator inhibitor type-1, CEA and CA 19-9 in colorectal cancer

    International Nuclear Information System (INIS)

    Cathepsin B and L (CATB, CATL), urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 play an important role in colorectal cancer invasion. The tumor marker utility and prognostic relevance of these proteases have not been evaluated in the same experimental setting and compared with that of CEA and CA-19-9. Protease, CEA and CA 19-9 serum or plasma levels were determined in 56 patients with colorectal cancer, 25 patients with ulcerative colitis, 26 patients with colorectal adenomas and 35 tumor-free control patients. Protease, CEA, CA 19-9 levels have been determined by ELISA and electrochemiluminescence immunoassay, respectively; their sensitivity, specificity, diagnostic accuracy have been calculated and correlated with clinicopathological staging. The protease antigen levels were significantly higher in colorectal cancer compared with other groups. Sensitivity of PAI-1 (94%), CATB (82%), uPA (69%), CATL (41%) were higher than those of CEA or CA 19-9 (30% and 18%, respectively). PAI-1, CATB and uPA demonstrated a better accuracy than CEA or CA 19-9. A combination of PAI-1 with CATB or uPA exhibited the highest sensitivity value (98%). High CATB, PAI-1, CEA and CA 19-9 levels correlated with advanced Dukes stages. CATB (P = 0.0004), CATL (P = 0.02), PAI-1 (P = 0.01) and CA 19-9 (P = 0.004) had a significant prognostic impact. PAI-1 (P = 0.001), CATB (P = 0.04) and CA 19-9 (P = 0.02) proved as independent prognostic variables. At the time of clinical detection proteases are more sensitive indicators for colorectal cancer than the commonly used tumor markers. Determinations of CATB, CATL and PAI-1 have a major prognostic impact in patients with colorectal cancer

  5. The Serum Level of Prostate Specific Antigen in Polycystic Ovary Syndrome

    International Nuclear Information System (INIS)

    To determine whether serum prostate specific antigen (PSA) level are increased in polycystic ovary syndrome (PCOS), 40 patients with PCOS, and 50 healthy control subjects were enrolled in the study.The subjects were compared by means of serum PSA T SHBG DHEA-S levels. The correlations between PSA and T SHBG DHEA-S were evaluated.Serum PSA levels were found to be significantly higher in PCOS (PSA: 15.64±3.36 pg/mL, in PCOS; PSA: 3.56±0.44pg/mL, in control; P<0.01). Positive correlations between PSA and T (r=0.467, P<0.01) and between PSA and DHEA-S (r=, 0.205 P<0.05) were found. A negative correlation between PSA and SHBG was apparent (r=-0.260, P<0.05). Females with PCOS tended to have higher PSA than females without PCOS (P<0.01). PSA appears to be a promising marker of androgen excess in females suffering from PCOS. (authors)

  6. Preparation of 99mTc-Anti CEA MAb and biodistribution test in mice

    International Nuclear Information System (INIS)

    99mTc-antiCEA MAb radiopharmaceutical has been commercially used under the brand name of Zevalin which is used to detect colorectal cancer. Although human MAb is preferred but commercial product is still using murine originated MAb. This antibody binds specifically to carcinoembryonic antigen (CEA) which is overexpressed in colorectal cancer cells. AntiCEA MAb was reduced with diluted 2-mercaptoethanol (1:2000) prior to labelling with 99mTc, and MDP was used as transchelating agent. Labeling efficiency was analysed with chromatography using HSA impregnated ITLC-SG as stationary phase and mixture of ammona-ethanol-water (1:2:5) as eluants to determine polar 99mTc impurities, and ITLC-SG eluted with salin to determine 99mTc-colloid. Stability study was carried out on radio labeled antiCEA MAb stored at room temperature within several hours and on reduced antiCEA MAb stored at -40°C for several weeks. Biodistribution of 99mTc-antiCEA MAb in normal mice was observed 1 hour and 4 hours post injection. Labeling efficiency of antiCEA MAb was 98,53% ± 0.21 % and decreasing to less than 90% after 9 weeks. Radiolabeled anti CEA MAb kept at room temperature was stable within 5 hours post injection, and the frozen kits were stable up to 9 weeks. Biodistribution of 99mTc-antiCEA MAb in normal mice at 1 hour and 4 hours post injection showed high uptake in various organs. (author)

  7. A rapid radioimmunoassay for determination of tumor antigens with reference to carcinoembryonic antigen

    International Nuclear Information System (INIS)

    A novel experimental procedure for the determination of carcinoembryonic antigen (CEA) by a solid phase microradioimmunoassay has been developed. Goat anti-CEA antibodies were immobilized by coupling to cyanogen bromide activated filter paper discs. The inhibition of binding of radioiodinated CEA to the discs was proportional to the amount of unlabelled CEA present in the test sample. The experimental procedure involved two steps: (i) the test material containing unlabelled CEA was allowed to react with the antibody coated discs at 37 deg C for 2 hrs., and (ii) a standard amount of 125I-CEA was added to the reaction mixture and incubated for 24 hours at 37 deg C or room temperature. The discs were then washed 4 times and the radioactivity of each disc was determined. The sensitivity of the test in its present state of development was 2.5 ng/ml. (author)

  8. Clinical significance of joint detection of serum CEA, SCCA, and bFGF in the diagnosis of lung cancer

    OpenAIRE

    Zhao, Wei; Yu, Haixiang; Han, Zhifeng; Gao, Nan; Xue, Jinru; Wang, Yan

    2015-01-01

    Lung cancer is a type of malignant tumor with highest morbidity and mortality. This study tested three tumor marker levels including CEA, SCCA, and bFGF to explore their value in lung cancer diagnosis and pathological type judgment. Venous blood was extracted from lung cancer patients, lung benign lesion patients and healthy control. Electrochemiluminescence immunoassay was applied to detect serum CEA and SCCA content. ELISA was used to test serum bFGF level. Serum CEA, SCCA, and bFGF levels ...

  9. Release of carcinoembryonic antigen from human colon cancer cells by phosphatidylinositol-specific phospholipase C.

    OpenAIRE

    Sack, T L; Gum, J R; Low, M G; Y. S. Kim

    1988-01-01

    Carcinoembryonic antigen (CEA) is released from colon cancer cells into the circulation where it is monitored clinically as an indicator of the recurrence or progression of cancer. We have studied the mechanism of CEA membrane attachment and release using the human colonic adenocarcinoma cell line LS-174T, specimens of human colon cancers, and serum from colon cancer patients. CEA release by cells in vitro and in vivo is associated with the conversion of CEA from a membrane-bound, hydrophobic...

  10. CEA - Assessment of risk management 2013

    International Nuclear Information System (INIS)

    After some introducing texts by CEA managers, this report proposes a rather detailed overview and presentation of CEA activities, objectives and obtained results in different fields: protection and control of the environment, installation safety, health, safety and radiation protection, transports of hazardous materials, waste management, protection of sites, installations and heritage, management of emergency situations, management of legal risks, internal controls and audits, activity of the risk management department, CEA activities from research to industry

  11. Clinical Significance and Prognostic Value of CA72-4 Compared with CEA and CA19-9 in Patients with Gastric Cancer

    Directory of Open Access Journals (Sweden)

    M. Ychou

    2000-01-01

    Full Text Available Carcinoembryonic antigen (CEA and CA 19-9 are both widely used in the follow up of patients with gastrointestinal cancer. More recently another tumor marker, named CA 72-4 has been identified and characterized using two different monoclonal antibodies B72.3 and CC49. Several reports evaluated CA 72-4 as a serum tumor marker for gastric cancer and compared its clinical utility with that of CEA or CA 19-9; few reports concerned its prognostic value. In the present study, CA 72-4 is evaluated and compared with CEA and CA 19-9 in various populations of patients with gastric cancer and benign disease; for 52 patients with gastric adenocarcinoma and 57 patients without neoplastic disease CEA, CA 19-9 and CA 72-4 were evaluated before treatment. Sensitivity of the tumor markers CA 72-4, CA 19-9 and CEA at the recommended cut-off level in all 52 patients were 58%, 50% the sensitivity increased to 75%. of these markers, for non metastatic patients, multivariate analyses indicated that none of the markers were significant, when adjusted for gender and age (which were indicators of poor prognosis; patients with abnormal values of CA72-4 tended to have shorter survival than patients with normal values (p < 0.07. In the metastatic population, only high values of CA19-9 (p < 0.02 and gender (women (p < 0.03 were indicators of poor prognosis in univariate analysis; multivariate analysis revealed that both CA72-4 (p = 0.034 and CA19-9 p = 0.009, adjusted for gender were independent prognostic factors. However, CA72-4 lost significance (p = 0.41 when adjusted for CA19-9 and gender, indicating that CA19-9 provides more prognostic information than CA72-4.

  12. FDG-PET scan in patients with clinically and/or radiologically suspicious colorectal cancer recurrence but normal CEA

    OpenAIRE

    Zhang Jun; Povoski Stephen P; Bloomston Mark; Sarikaya Ismet; Hall Nathan C; Knopp Michael V; Martin Edward W

    2007-01-01

    Abstract Background Although frequently used for tumor surveillance, the sensitivity of carcinoembryonic antigen (CEA) to detect recurrent colorectal cancer (CRC) is not optimal. Fluorine 18-fluoro-2-deoxy-glucose-positron emission tomography (18F FDG-PET) scans promise to improve recurrent CRC detection. We aimed to review PET scans of patients with clinically and/or radiologically suspicious tumor recurrence but normal CEA. Methods A retrospective review of an electronic database of 308 pat...

  13. Las Gramináceas

    Directory of Open Access Journals (Sweden)

    Robledo Emilio

    1939-08-01

    Full Text Available Las gramináceas son plantas conocidas desde la antigüedad más remota. Tan importantes son las gramináceas desde el punto de vista bromatológico, que los hombres pueden ser clasificados en comedores de trigo, de arroz, de maíz, de mijo, de sorgo, de tocusso, de tef, etc. El área de distribución de las gramíneas es inmensa: se las encuentra desde las orillas del mar y en las aguas dulces, hasta las nieves perpetuas; sólo en las aguas saladas desconocidas. En punto de tamaño, las hay desde unos centímetros hasta nuestra guadua gigantesca, de treinta y más metros de altura. Dicha distribución se halla en relación con el clima, pero también es influida por los hábitos de los pueblos, por la civilización, por el comercio o por otras circunstancias.

  14. Application of monoclonal antibodies to purified CEA in clinical radioimmunoassay of human serum

    International Nuclear Information System (INIS)

    Double-antibody radioimmunoassay using a mouse monoclonal anti- CEA (MA/1) has been used to measure CEA in human serum. Low levels of MA/1-binding CEA have been found in serum from normal individuals and moderately raised levels are sometimes associated with certain non-malignant diseases. As with conventional anti-CEA, the MA/1 antibodies can recognize significant amounts of CEA in serum from patients with a variety of solid tumours. However they appear to recognize a different immunodeterminant and possibly a different population of CEA molecules to, or a subset of, those measured by two routine assays. Studies in which the MA/1 assay was directly compared with the results of the Charing Cross routine and Abbott EIA assays have indicated that different immunological forms of CEA may be expressed in the course of tumour progression but no prognostic value was evident in this study. The results stress the need to resolve immunological specificities expressed by CEA-like molecules and evaluate their clinical importance. (author)

  15. Body mass index in relation to serum prostate-specific antigen levels and prostate cancer risk.

    Science.gov (United States)

    Bonn, Stephanie E; Sjölander, Arvid; Tillander, Annika; Wiklund, Fredrik; Grönberg, Henrik; Bälter, Katarina

    2016-07-01

    High Body mass index (BMI) has been directly associated with risk of aggressive or fatal prostate cancer. One possible explanation may be an effect of BMI on serum levels of prostate-specific antigen (PSA). To study the association between BMI and serum PSA as well as prostate cancer risk, a large cohort of men without prostate cancer at baseline was followed prospectively for prostate cancer diagnoses until 2015. Serum PSA and BMI were assessed among 15,827 men at baseline in 2010-2012. During follow-up, 735 men were diagnosed with prostate cancer with 282 (38.4%) classified as high-grade cancers. Multivariable linear regression models and natural cubic linear regression splines were fitted for analyses of BMI and log-PSA. For risk analysis, Cox proportional hazards regression models were used to estimate hazard ratios (HR) and 95% confidence intervals (CI) and natural cubic Cox regression splines producing standardized cancer-free probabilities were fitted. Results showed that baseline Serum PSA decreased by 1.6% (95% CI: -2.1 to -1.1) with every one unit increase in BMI. Statistically significant decreases of 3.7, 11.7 and 32.3% were seen for increasing BMI-categories of 25 prostate cancer risk although results were indicative of a positive association to incidence rates of high-grade disease and an inverse association to incidence of low-grade disease. However, findings regarding risk are limited by the short follow-up time. In conclusion, BMI was inversely associated to PSA-levels. BMI should be taken into consideration when referring men to a prostate biopsy based on serum PSA-levels. PMID:26914149

  16. Avaliação dos valores sérico e pleural dos marcadores tumorais CEA, CYFRA21-1 e CA 15-3 em portadores de derrame pleural Evaluation of serum and pleural levels of the tumor markers CEA, CYFRA21-1 and CA 15-3 in patients with pleural effusion

    OpenAIRE

    Isabella Coimbra Wagner; Murilo José de Barros Guimarães; Lilian Karine Neves da Silva; Francisco Montenegro de Melo; Maria Tereza Cartaxo Muniz

    2007-01-01

    OBJETIVO: Dosar os marcadores tumorais antígeno carcinoembrionário (CEA), fragmento da citoqueratina 19 (CYFRA21-1) e antígeno glicosídico associado a tumor 15-3 (CA 15-3) em sangue e líquido pleural de portadores de derrames pleurais benignos e malignos, avaliando a sensibilidade de cada um deles nesses fluidos. MÉTODOS: Avaliamos prospectivamente 85 pacientes com derrame pleural. O estudo do líquido pleural obedeceu a critérios determinados pela literatura. A dosagem dos marcadores foi real...

  17. Establishment of 131I, 99mTc Labeling Methods to In-house Anti-CEA Antibodies and Evaluation of the Immunological Characteristics

    International Nuclear Information System (INIS)

    Cancer cells have several tumor-associated antigens on the cell surfaces, and antibodies against these antigens have been developed by many investigators. Radiolabeled antibodies have been used as new methods to diagnose and treat malignant tumors. Especially anti-carcinoembryonic antigen (CEA) is the most popular antibody for these purposes. In this investigation, we tried to label 131I and 99mTc to anti-CEA monoclonal antibodies which were developed in the Seoul National University College of Medicine. We found CEA-79 and CEA-92 antibodies had the better immunological characteristics among 8 anti-CEA monoclonal antibodies. And radioiodination of CEA-79 could be performed by chloramine-T method, while radioiodination of CEA-92 by iodogen method. To label these antibodies with 99mTc, we used pretargeting transchelation as direct labeling method. At first, 99mTc was bound to glucaric acid, and monoclonal antibody was reduced by β-mercaptoethanol. When these were incubated together, 99mTc bound to glucarate was switched to monoclonal antibody because of higher affinity. We established conditions of several steps in this method. Anti-CEA monoclonal antibodies labeled with 131I and 99mTc are expected to be used valuably in the detection and treatment of malignant tumors.

  18. Saclay CEA Centre. Environmental assessment 2009

    International Nuclear Information System (INIS)

    This report presents the environmental control organization (sampling places and frequencies) about the Saclay CEA centre, and indicates the authorized levels for liquid and gaseous effluent releases by its installations. It comments the obtained results concerning air quality, water quality (surface and underground waters), bio-indicators (sampling and measurements performed in grasses, fruits, vegetables, milk, and so on, with notably the presence of Tritium of K40), and the assessment of the radiological impact due to annual radioactive releases. The report discusses the evolution of Tritium releases since 1968, and gives an assessment of these releases before 1968 (i.e. before it has been systematically controlled). It comments the new regulation regarding releases and gives a comparison with the releases limits defined in 1978. A brief presentation of the Saclay center environmental policy is given

  19. Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein

    International Nuclear Information System (INIS)

    CEA is a tumor-associated antigen abundantly expressed on several cancer types, including those naturally refractory to chemotherapy. The selection and characterization of human anti-CEA single-chain antibody fragments (scFv) is a first step toward the construction of new anticancer monoclonal antibodies designed for optimal blood clearance and tumor penetration. The human MA39 scFv, selected for its ability to recognize a CEA epitope expressed on human colon carcinomas, was first isolated from a large semi-synthetic ETH-2 antibody phage library, panned on human purified CEA protein. Subsequently, by in vitro mutagenesis of a gene encoding for the scFv MA39, a new library was established, and new scFv antibodies with improved affinity towards the CEA cognate epitope were selected and characterized. The scFv MA39 antibody was affinity-maturated by in vitro mutagenesis and the new scFv clone, E8, was isolated, typed for CEA family member recognition and its CEACAM1, 3 and 5 shared epitope characterized for expression in a large panel of human normal and tumor tissues and cells. The binding affinity of the scFv E8 is in a range for efficient, in vivo, antigen capture in tumor cells expressing a shared epitope of the CEACAM1, 3 and 5 proteins. This new immunoreagent meets all criteria for a potential anticancer compound: it is human, hence poorly or not at all immunogenic, and it binds selectively and with good affinity to the CEA epitope expressed by metastatic melanoma and colon and lung carcinomas. Furthermore, its small molecular size should provide for efficient tissue penetration, yet give rapid plasma clearance

  20. DOE–CEA Benchmark on SFR ASTRID Innovative Core: Neutronic and Safety Transients Simulation

    International Nuclear Information System (INIS)

    ASTRID is a fast reactor being designed by the CEA to achieve a level of safety that exceeds that of conventional fast reactors. In particular, an axially heterogeneous core with an upper sodium plenum is employed to achieve a non-positive sodium void reactivity worth. In order to address the simulation challenges for this innovative concept, the US Department of Energy’s (DOE) Laboratories (Argonne National Laboratory and Idaho National Laboratory) and the CEA are performing neutronic and transient benchmark calculations for an ASTRID model based on design specifications provided by the CEA. The blind comparison of the initial DOE and CEA results are found to be in good agreement, enhancing confidence in CEA predictions of key ASTRID safety relevant parameters and transient behaviour. For several parameters, compared uncertainties in computed values are significant and further studies are needed to reduce them. (author)

  1. Decontamination and dismantling at the CEA

    International Nuclear Information System (INIS)

    This document presents the dismantling policy at the CEA (French Research Center on the atomic energy), the financing of the decontamination and the dismantling, the regulatory framework, the knowledge and the technology developed at the CEA, the radiation protection, the environment monitoring and the installations. (A.L.B.)

  2. Memoirs of a Cea veteran

    International Nuclear Information System (INIS)

    A brief account is given of the way in which nuclear energy has developed in France and elsewhere over the last fifty years: options developed by the French Atomic Energy Commission (CEA), naval propulsion, development of pressurised water reactors, MOX and thorium fuels. Afterwards, the prospectives for the 21. century will be discussed. Considering that natural resources are depleting while releases of both greenhouse gases and world population are increasing, an active energy policy will have to be implemented with due consideration for social equity and solidarity. It is in this context that the developed countries will have to give preference, beyond savings, to renewable sources of energy, including of course, nuclear energy. Nuclear energy can continue to develop in the long term, provided fast breeder technology is developed at some point. As far as transport is concerned, hydrogen technology, which is clean and renewable, is promising, provided it is generated by nuclear energy. (author)

  3. CEA - 2012 Annual Report, 2012 Financial Statements

    International Nuclear Information System (INIS)

    In its first part, this report proposes an overview of activities within the CEA. They concern the CEA's programs on low on carbon energies and associated fundamental researches, on defence and global security and associated fundamental researches, on information technologies and associated fundamental researches, on health technologies and associated fundamental researches, and on very large research infrastructures and associated fundamental researches. The second part addresses the scientific assessment, activities related to teaching and training, to innovation towards enterprises, and to support to valorization. It also indicates prices awarded to the CEA. The third part addresses CEA management and institutional relationships, human resources, international relationships, activities related to communication and information diffusion, and risk management. The fourth part describes the CEA organization, its governance and its various bodies. The second volume contains the financial statements for 2012

  4. 188Re-iorCEA1 as radioimmunotherapeutical agent: Preparation and evaluation. Cuba

    International Nuclear Information System (INIS)

    Effective immunotherapy of human malignancies requires the selective delivery of these agents to relevant antigen-expressing tumour cells. A variety of strategies have been investigated to achieve this objective. It has been very clearly established that ior-CEA1 monoclonal antibody is an extremely good agent for immunoscintigraphy with a high sensitivity (94%) and specificity (87%). Ior-CEA1 is hence a good candidate for development as a therapeutic agent. Moreover, it is an attractive novel protein, which conserves its biological properties, despite chemical treatment

  5. Sensitivity and specificity of the GAMMACOAT-M CEA-RIA from Travenol-Genentech Diagnostics compared to the ABBOTT CEA-RIA monoclonal

    International Nuclear Information System (INIS)

    A new monoclonal radioimmunoassay from Travenol-Genentech Diagnostics was investigated with respect to its sensitivity and specificity and compared to the established monoclonal antibody assay of ABBOTT. Both tests revealed increased CEA plasma values in healthy smokers, whereby the elevation was dependent on the number of cigarettes smoked per day. The sensitivities as well as the cut-off levels for CEA at 90% specificity were coincident in both tests. (orig.)

  6. Application of VLLW management principles to the CEA research centers

    International Nuclear Information System (INIS)

    This document describes the content of a CEA policy relating to very low level waste management elaborated in application of the waste management principles defined in France. The policy deals with very low level waste, subject to recycling, incineration or landfill disposal. It does not deal with reuse. The following principles are applicable to waste streams produced by CEA nuclear installations either during operating or dismantling activities. The policy deals only with very low level wastes (VLLW) (order of magnitude: <100 Bq/g for high energy emitters). It does not deal with low, intermediate or high level waste, which are either recycled or incinerated in nuclear industry or disposed of in the Aube surface disposal (CSA) or kept in intermediate storage, before geological disposal or any alternative final solution

  7. Recombinant carcinoembryonic antigen as a reporter gene for molecular imaging

    Energy Technology Data Exchange (ETDEWEB)

    Kenanova, Vania; Barat, Bhaswati; Olafsen, Tove; Chatziioannou, Arion; Herschman, Harvey R.; Wu, Anna M. [David Geffen School of Medicine at the University of California Los Angeles, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Los Angeles, CA (United States); Braun, Jonathan [David Geffen School of Medicine at the University of California Los Angeles, Department of Pathology and Laboratory Medicine, Los Angeles, CA (United States)

    2009-01-15

    Reporter genes can provide a way of noninvasively assessing gene activity in vivo. However, current reporter gene strategies may be limited by the immunogenicity of foreign reporter proteins, endogenous expression, or unwanted biological activity. We have developed a reporter gene based on carcinoembryonic antigen (CEA), a human protein with limited normal tissue expression. To construct a CEA reporter gene for PET, a CEA minigene (N-A3) was fused to the extracellular and transmembrane domains of the human Fc{gamma}RIIb receptor. The NA3-Fc{gamma}RIIb recombinant gene, driven by a CMV promoter, was transfected in Jurkat (human T cell leukemia) cells. Expression was analyzed by flow cytometry, immunohistochemistry (IHC), and microPET imaging. Flow cytometry identified Jurkat clones stably expressing NA3-Fc{gamma}RIIb at low, medium, and high levels. High and medium NA3-Fc{gamma}RIIb expression could also be detected by Western blot. Reporter gene positive and negative Jurkat cells were used to establish xenografts in athymic mice. IHC showed staining of the tumor with high reporter gene expression; medium and low N-A3 expression was not detected. MicroPET imaging, using an anti-CEA {sup 124}I-labeled single-chain Fv-Fc antibody fragment, demonstrated that only high N-A3 expression could be detected. Specific accumulation of activity was visualized at the N-A3 positive tumor as early as 4 h. MicroPET image quantitation showed tumor activity of 1.8 {+-} 0.2, 15.2 {+-} 1.3, and 4.6 {+-} 1.2 percent injected dose per gram (%ID/g) at 4, 20, and 48 h, respectively. Biodistribution at 48 h demonstrated tumor uptake of 4.8 {+-} 0.8%ID/g. The CEA N-A3 minigene has the potential to be used as a reporter gene for imaging cells in vivo. (orig.)

  8. 血清CEA,CA125,NSE,CYFRA21-1联合检测对肺癌的诊断价值%Diagnostic Value of Combined Determination of Serum Tumor Markers (CEA, CA 125, NSE, CYFRA 21-1 ) Levels in Patients with Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    黄芳; 耿燕; 李婷婷; 张西英

    2008-01-01

    目的 探讨血清癌胚抗原(CEA)、糖类抗原125(CA125)、细胞角蛋白片段21-1(CYFRA21-1)、神经元特异性烯醇化酶(NSE)联合检测对肺癌的诊断价值.方法 采用电化学发光法对78例肺癌(其中鳞癌30例,腺癌28例,小细胞癌20例)、45例肺部良性病患者和36例健康人血清进行分析.结果 肺癌患者血清4种肿瘤标志物含量明显高于肺部良性疾病组(P<0.05)和健康对照组(P<0.01).肺癌组经病理分型后,NSE在小细胞肺癌中的水平明显高于其他类型的肺癌(P<0.05);CYFRA21-1的水平在鳞癌中升高尤为明显(P<0.05);CEA的水平在腺癌中显著升高(P<0.05).4种肿瘤标志物单项检测时,特异度较高(82.22%~95.56%),但敏感度不是很高(38.46%~56.41%).联合检测后,可提高肺癌的阳性检出率(64.10%).结论 CEA,CA125,NSE和CYFRA21-1对肺癌有一定的辅助诊断价值,且CEA,NSE和CYFRA21-1对不同组织类型肺癌均有一定诊断价值,联合检测可提高对肺癌的阳性诊断.

  9. Factors related to serum level of carbohydrate antigen 19-9 and cancer antigen 125 in healthy rural populations in Korea

    International Nuclear Information System (INIS)

    This study examines the levels of carbohydrate antigen 19-9(CA 19-9) and cancer antigen 125(CA125) in serum and its related factors in healthy Korean population. Although CA19-9 and CA125 have been widely used tumor markers for gastroenteric cancers and ovarian cancer in Western countries, there are no information available on the serum levels of CA19-9 and CA125 in healthy population and the factors affecting the levels of these tumor markers in Korea. A cross-sectional study was performed to measure CA19-9 and CA125 among 76 healthy males and 95 healthy females in Korea. CA19-9 and CA125 were quantitated using solid-phase radioimmunoassay kits. Informations on the factors which might be related to the levels of these markers were collected by questionnaire(e.g., smoking, alcohol consumption, menstruation, oral pill use, breast-feeding history, etc.). There was no statistically significant difference in the mean of CA19-9 concentration between men(10.4 u/ml) and women (10.1 u/ml), whereas the mean of CA125 levels (11.2 u/ml) was higher in women than that(2.5 u/ml) in men. Although there was a statistically significant association between CA19-9 and average number of cigarette consumed per day (r=0.59, p=0.026) and total number of cigarettes consumed in women (r=0.74, p=0.003), the significance disappeared by multiple regression analysis after adjusting age and body mass index. Later age of menopause(p=0.035) and longer duration of breast-feeding(p=0.050) were significant predictors for CA125 levels in women by multiple regression analysis after adjusting age and body mass index. In conclusion, CA19-9 can be used as a stable tumor marker in clinical practices, however, menstruation and breast-feeding should be considered when CA125 is used in women

  10. Combination of IGF-1 with CEA, CYFRA21-1, NSE for the diagnosis and prediction of treatment response in lung cancer

    International Nuclear Information System (INIS)

    Objective: To evaluate four tumor markers of insulin-like growth factor 1((IGF-1), CEA, cytokeratin fragment antigen 21-1 (CYFRA21-1), neuron-specific enolase (NSE) for the diagnosis and prediction of treatment response in human lung cancer. Methods: Serum samples were taken from three groups: 91 patients with lung cancer, 30 healthy adults and 15 patients with benign pulmonary diseases. Serum IGF-1 was assayed by radioimmunoassay and CEA, CYFRA21-1, and NSE by electrochemiluminescence immunoassay. The differences among the three groups were determined by Kruskal-Wallis one-way analysis of variance (ANOVA) and with Mann-Whitney rank-sum test. Diagnostic efficacy was evaluated by ROC curves. Results: The four serum tumor marker levels were significantly higher in lung cancer group, as compared with the benign and the healthy (IGF-1: χ2=26.95, P2=49.11, P2=40.63, P2=14.76; P2=5.99, P=0.014; CYFRA21-1: χ2=4.99, P=0.025) in cancer group. Conclusions: Serum IGF-1, CEA, CYFRA21-1 and NSE are all valuable for lung cancer diagnosis and the combination of those parameters can enhance the diagnostic efficiency. Serum IGF-1 and CYFRA21-1 may also be useful for evaluating the treatment response in lung cancer. (authors)

  11. Antibody-guided irradiation of hepatic metastases using intrahepatically administered radiolabelled anti-CEA antibodies with simultaneous and reversible hepatic blood flow stasis using biodegradable starch microspheres.

    Science.gov (United States)

    Epenetos, A A; Courtenay-Luck, N; Dhokia, B; Snook, D; Hooker, G; Lavender, J P; Hemmingway, A; Carr, D; Paraharalambous, M; Bosslet, K

    1987-12-01

    Two monoclonal antibodies to carcinoembryonic antigen (CEA) were radiolabelled with 131I and used for the treatment of hepatic metastases in a patient who had a primary colonic carcinoma. Approximately 100 mCi of 131I-labelled antibody were administered via the hepatic artery on two occasions. On the second occasion, radiolabelled antibody was given concurrently with biodegradable starch microspheres in an attempt to enhance tumour uptake of antibody by achieving temporary stasis or delay of hepatic blood flow. The procedure was carried out uneventfully. There was clinical improvement and a fall in circulating CEA levels after each course of treatment. Furthermore, after the second course of therapy the clinical improvement was sustained for a longer period (more than 3 months) and there was evidence of diminution in the size of some of the liver metastases. Regional administration of 131I-labelled anti-CEA antibody concurrently with biodegradable starch microspheres appears to be a promising new method for the treatment of hepatic metastases from colonic carcinoma. PMID:3449789

  12. CEA - 2011 annual report, 2011 financial statement

    International Nuclear Information System (INIS)

    The first report, available both in French and English, presents the different current programs: low carbon energies and associated fundamental researches, global defence and safety and associated researches, information technologies and associated researches, technologies for health and associated fundamental researches, very large research infrastructures and associated fundamental researches. It then addresses the CEA openness: assessment, teaching and training, research valorisation, awards, and the support to various programs: steering activity by the CEA, human resources, international relationships, communication, risk management, information systems. The last part describes the CEA organisation. The second report presents the different financial and accounting data and tables

  13. CEA contribution to advanced robotics

    International Nuclear Information System (INIS)

    Due to development of nuclear energy in France, CEA has a long experience in nuclear robotics. It started from remote handling in hot cells and extended to inspections in nuclear reactors, intervention with vehicles, decommissioning of experimental plants, maintenance and intervention in reactors and reprocessing plants. Existing equipments, which are briefly described, show present status of performances for these applications. Research activities are going-on to develop this potential. Main aspects of recent advances in nuclear robotics are detailed to show that each application has some relevance with fusion remote handling needs. In this panel of activities, fusion would profit more directly from reprocessing and reactor maintenance studies, Computer Aided Teleoperation and advanced manipulators developments. But all the projects in nuclear and servicing robotics may have spin-offs for fusion and mention to Eureka projects will underline the necessity for European cooperation in this field. Finally, honor will be rendered to Jean Vertut who had from the first moment a real passion for fusion. (author). 3 refs, 4 figs, 2 tabs

  14. Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer

    Directory of Open Access Journals (Sweden)

    Campos da Paz M

    2012-10-01

    Full Text Available Mariana Campos da Paz,1 Maria de Fátima M Almeida Santos,1 Camila MB Santos,2 Sebastião W da Silva,2 Lincoln Bernardo de Souza,3 Emília CD Lima,3 Renata C Silva,1 Carolina M Lucci,1 Paulo César Morais,2 Ricardo B Azevedo,1 Zulmira GM Lacava11Instituto de Ciências Biológicas; 2Instituto de Física, Universidade de Brasília, Brasília, DF, Brazil; 3Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, BrazilAbstract: Nanosized maghemite particles were synthesized, precoated (with dimercaptosuccinic acid and surface-functionalized with anticarcinoembryonic antigen (anti-CEA and successfully used to target cell lines expressing the CEA, characteristic of colorectal cancer (CRC cells. The as-developed nanosized material device, consisting of surface decorated maghemite nanoparticles suspended as a biocompatible magnetic fluid (MF sample, labeled MF-anti-CEA, was characterized and tested against two cell lines: a high-CEA expressing cell line (LS174T and a low-CEA expressing cell line (HCT116. Whereas X-ray diffraction was used to assess the average core size of the as-synthesized maghemite particles (average 8.3 nm in diameter, dynamic light scattering and electrophoretic mobility measurements were used to obtain the average hydrodynamic diameter (550 nm and the zeta-potential (−38 mV of the as-prepared and maghemite-based nanosized device, respectively. Additionally, surface-enhanced Raman spectroscopy (SERS was used to track the surface decoration of the nanosized maghemite particles from the very first precoating up to the attachment of the anti-CEA moiety. The Raman peak at 1655 cm−1, absent in the free anti-CEA spectrum, is the signature of the anti-CEA binding onto the precoated magnetic nanoparticles. Whereas MTT assay was used to confirm the low cell toxicity of the MF-anti-CEA device, ELISA and Prussian blue iron staining tests performed with both cell lines (LS174T and HCT116 confirm that the as-prepared MF-anti-CEA

  15. Identification of a Carcinoembryonic Antigen Gene Family in the Rat

    OpenAIRE

    Kodelja, Vitam; Lucas, Kurt; Barnert, Sabine; Kleist, Sabine von; Thompson, John A.; Zimmermann, Wolfgang

    1989-01-01

    The existence of a carcinoembryonic antigen (CEA)-like gene family in rat has been demonstrated through isolation and sequencing of the N- terminal domain exons of presumably five discrete genes (rnCGM1-5). This finding will allow for the first time the study of functional and clinical aspects of the tumor marker CEA and related antigens in an animal model. Sequence comparison with the corresponding regions of members of the human CEA gene family revealed a relatively low similarity at the am...

  16. The clinical value of 99Tcm-HL91 hypoxia imaging combined with the detection of serum NSE, CEA and CYFRA21-1 in the diagnosis of lung cancer

    International Nuclear Information System (INIS)

    Objective: To investigate the value of 99Tcm-HL91 hypoxia imaging combined the detection of serum neuron-specific enolase (NSE), carcinoembryonic antigen(CEA) and cytokeratin 19 fragment (CYFRA21-1) in the diagnosis of lung cancer. Methods: 63 patients with lung neoplasm were carried out 99Tcm-Hail hypoxia imaging, and the serum tumor markers levels of Nose, Cea and CYFRA21-1 were detected. The sensitivity, specificity and accuracy of each and combined method were calculated respectively and compared with each other. Results: The sensitivity,specificity and accuracy in diagnosis of lung cancer with 99Tcm-HL91 hypoxia imaging were 85.4%, 100% and 88.9% respectively; and with serum tumor markers NSE, CEA and CYFRA21-1 detection were 72.9%, 86.7% and 76.1% respectively. Compared with the detection of serum tumor marker levels of NSE, CEA and CYFRA21-1, 99Tcm-HL91 hypoxia imaging had the higher sensitivity and specificity,and similar accuracy. The sensitivity, specificity and accuracy of combined two methods in diagnosis of lung cancer were 95.8%, 86.7% and 93.6% respectively. The sensitivity and accuracy of the combined method were significantly increased than single method (P0.05). Conclusion: 99Tcm-HL91 hypoxia imaging and the detection of serum level of NSE, CEA and CYFRA21-1 have an important diagnostic value, and their combination could improve the sensitivity and accuracy in the diagnosis of lung cancer. (authors)

  17. Physiological level production of antigen-specific human immunoglobulin in cloned transchromosomic cattle.

    Directory of Open Access Journals (Sweden)

    Akiko Sano

    Full Text Available Therapeutic human polyclonal antibodies (hpAbs derived from pooled plasma from human donors are Food and Drug Administration approved biologics used in the treatment of a variety of human diseases. Powered by the natural diversity of immune response, hpAbs are effective in treating diseases caused by complex or quickly-evolving antigens such as viruses. We previously showed that transchromosomic (Tc cattle carrying a human artificial chromosome (HAC comprising the entire unrearranged human immunoglobulin heavy-chain (hIGH and kappa-chain (hIGK germline loci (named as κHAC are capable of producing functional hpAbs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, are homozygously inactivated (double knockouts or DKO. However, B lymphocyte development in these Tc cattle is compromised, and the overall production of hpAbs is low. Here, we report the construction of an improved HAC, designated as cKSL-HACΔ, by incorporating all of the human immunoglobulin germline loci into the HAC. Furthermore, for avoiding the possible human-bovine interspecies incompatibility between the human immunoglobulin mu chain protein (hIgM and bovine transmembrane α and β immunoglobulins (bIgα and bIgβ in the pre-B cell receptor (pre-BCR complex, we partially replaced (bovinized the hIgM constant domain with the counterpart of bovine IgM (bIgM that is involved in the interaction between bIgM and bIgα/Igβ; human IgM bovinization would also improve the functionality of hIgM in supporting B cell activation and proliferation. We also report the successful production of DKO Tc cattle carrying the cKSL-HACΔ (cKSL-HACΔ/DKO, the dramatic improvement of B cell development in these cattle and the high level production of hpAbs (as measured for the human IgG isotype in the plasma. We further demonstrate that, upon immunization by tumor immunogens, high titer tumor immunogen-specific human IgG (hIgG can be produced from such Tc cattle.

  18. Cytokeratin 19 fragment antigen 21-1 as an independent predictor for definitive chemoradiotherapy sensitivity in esophageal squamous cell carcinoma

    Institute of Scientific and Technical Information of China (English)

    YAN Hong-jiang; WANG Ren-ben; ZHU Kun-li; JIANG Shu-mei; ZHAO Wei; XU Xiao-qing; FENG Rui

    2012-01-01

    Background Patients with esophageal squamous cell carcinoma (ESCC) undergoing definitive chemoradiotherapy (CRT) seem to have a disparity in therapeutic response.The identification of CRT sensitivity-related clinicopathological factors would be helpful for selecting patients most likely to benefit from CRT.Cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) and carcinoembryonic antigen (CEA) have been reported as useful tumor markers for esophageal cancer.The aim of this study was to examine the predictive value of CYFRA21-1 in comparison with CEA and other clinicopathological factors in patients with ESCC treated with definitive CRT.Methods Pretreatment serum CYFRA21-1 and CEA levels were measured by immunoradiometric assays.The relationships between pretreatment clinicopathological factors and the efficacy of CRT were analyzed.Overall survival (OS) was estimated by univariate and multivariate analysis.Results The results from a univariate analysis indicated that the efficacy of CRT was significantly associated with the serum levels of CYFRA21-1 and CEA before treatment (P=0.001 and P=0.023,respectively).It also indicated that the efficacy of CRT was significantly associated with the pretreatment tumor location (P=0.041).By Logistic regression analysis,the independent predictive factor associated with efficacy of CRT was CYFRA21-1 (P=0.002).The OS of the patients with high CYFRA 21-1 levels was worse than that of those with low CYFRA21-1 levels (P=0.001).In multivariate analysis,a low level of CYFRA21-1 was the most significant independent predictor of good OS (P=0.007).Conclusions CEA and tumor location may be useful in predicting the sensitivity of ESCC to CRT.CYFRA21-1 may be an independent predictor for definitive CRT sensitivity in ESCC.

  19. CEA - Assessment of risk management for 2012

    International Nuclear Information System (INIS)

    This report proposes an overview of the main events, actions performed by the CEA, and facts for 2012 regarding protection and monitoring of the environment, installation safety, occupational health and safety, radiological protection of workers, transportation of hazardous materials, waste management, protection of sites, installations and heritage, emergency situation management, legal risk management, internal controls and audits. It also presents the organisation and action of the risk management department within the CEA

  20. The CEA-Industrie Group of Companies

    International Nuclear Information System (INIS)

    The 1988 financial and technological status of the CEA-Industry Group of Companies is summarized. The activities, technological innovations, and areas of development perspectives of the CEA-Industry Group of Companies, chiefly concentrated in fields relating to nuclear energy, are described. The principal business sectors of the group involve nuclear fuel cycle, nuclear plants and maintenance, computer applications and life science. Some activities of the group are extended to management, construction and financial fields

  1. 1999 scientific evaluation at the CEA

    International Nuclear Information System (INIS)

    This report presents a statement of the scientific and technical activity of the French atomic energy commission (CEA) for the year 1999. This evaluation is made by external and independent experts and requires some specific dispositions for the nuclear protection and safety institute (IPSN) and for the direction of military applications (DAM). The report is divided into 4 parts dealing successively with: 1)the CEA, a public research organization (strategy, research programs, new organization of the CEA activities, civil nuclear research, technology research and transfer, defence activities, transfer of knowledge) 2)the scientific evaluation at the CEA (evaluations of the civil applications of the CEA, IPSN, DAM, INSTN (national institute for nuclear sciences and techniques) 3)synthesis of the 1999 scientific and technical evaluation for each operational directions of the CEA (directions of fuel cycle, of nuclear reactors, of advanced technologies, of materials sciences, of life sciences, of military applications, of the nuclear protection and safety institute and of the national institute for nuclear sciences and techniques) 4)the corresponding members of the evaluation and the list of scientific and technical councils and members

  2. RHAMM (CD168 Is Overexpressed at the Protein Level and May Constitute an Immunogenic Antigen in Advanced Prostate Cancer Disease

    Directory of Open Access Journals (Sweden)

    Kilian M. Gust

    2009-09-01

    Full Text Available Localized prostate cancer (CaP can be cured using several strategies. However, the need to identify active substances in advanced tumor stages is tremendous, as the outcome in such cases is still disappointing. One approach is to deliver human tumor antigen-targeted therapy, which is recognized by T cells or antibodies. We used data mining of the Cancer Immunome Database (CID, which comprises potential immunologic targets identified by serological screening of expression libraries. Candidate antigens were screened by DNA microarrays. Genes were then validated at the protein level by tissue microarrays, representing various stages of CaP disease. Of 43 targets identified by CID, 10 showed an overexpression on the complementary DNA array in CaP metastases. The RHAMM (CD168 gene, earlier identified by our group as an immunogenic antigen in acute and chronic leukemia, also showed highly significant overexpression in CaP metastases compared with localized disease and benign prostatic hyperplasia. At the protein level, RHAMM was highest in metastatic tissue samples and significantly higher in neoplastic localized disease compared with benign tissue. High RHAMM expression was associated with clinical parameters known to be linked to better clinical outcome. Patients with high RHAMM expression in the primaries had a significantly lower risk of biochemical failure. The number of viable cells in cell cultures was reduced in blocking experiments using hormone-sensitive and hormone-insensitive metastatic CaP cell lines. Acknowledging the proven immunogenic effects of RHAMM in leukemia, this antigen is intriguing as a therapeutic target in far-advanced CaP.

  3. A prospective study of serum tumour markers carcinoembryonic antigen, carbohydrate antigens 50 and 242, tissue polypeptide antigen and tissue polypeptide specific antigen in the diagnosis of pancreatic cancer with special reference to multivariate diagnostic score.

    OpenAIRE

    Pasanen, P. A.; Eskelinen, M.; Partanen, K.; Pikkarainen, P; Penttilä, I.; Alhava, E

    1994-01-01

    The aim of this study was to assess by a stepwise multivariate discriminant analysis the value of four current serum tumour markers - carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 50 and CA 242 and tissue polypeptide antigen (TPA) - and a new serum tumour marker, tissue polypeptide specific antigen (TPS), in the diagnosis of pancreatic cancer. The serum values were measured in a prospective series of patients with jaundice, with unjaundiced cholestasis and with a suspicion of chro...

  4. Radioiodination of monoclonal antibody intact anti-CEA

    International Nuclear Information System (INIS)

    The purpose of this study is to examine a convenient system that can be used to iodinate monoclonal antibodies which is rapid, simple, efficient and reproducible, and which can be accomplished in radiopharmaceutical laboratories. It is important to remember that antibodies are sensitive biochemicals, subject to losses of the activity that is essential to their mode of action, namely the ability to bind specific antigen. The advent of solid phase iodination agents has greatly expanded the range of gentle iodination techniques available for iodinating sensitive biological materials. The agent most widely used is the Iodogen (1,3,4,6 tetrachloro-3a-6a diphenylglycoluril) method. Anti-CEA 4C sub(11) IgG sub(2a,k) (prepared in the Ludwig Institute-Sao Paulo-Brazil ) is used as model to evaluate the Iodogen methodology. The miniature chromatographic system, also rapid, accurate, simple, efficient was elaborated to determine the labelling efficiency incorporation of iodine into immunoglobulin, and the radiochemical purity of sup(131)I-anti-CEA. (author)

  5. Summary 1998. Releases control and environment monitoring for the CEA Centers

    International Nuclear Information System (INIS)

    In the framework of its environmental policy, the CEA aims at reduce as weak as possible, in regards to the technological and economic needs, its activities impacts on the people and the environment. This paper contributes to the public information on the radioactive gaseous and liquid releases during the year 1998. It presents data on the releases and the radioactivity levels around the CEA sites and gathers the associated regulation and monitoring methods. (A.L.B.)

  6. CEA nuclear energy Directorate - Activity report 2012

    International Nuclear Information System (INIS)

    After an overview of the activities of the Directorate at the international level, of its scientific activities, and of the consideration given to quality, and a presentation of the transverse program on advanced materials, this report proposes presentations of activities in different domains: future nuclear industrial systems (reactors of 4. generation, back-end of the future cycle, sustainable management of nuclear materials, fundamental scientific and technological research), optimization of the present industrial nuclear activity (reactors of 2. and 3. generation, front-end and back-end of the fuel cycle), the main tools for nuclear development (numerical simulation, the Jules Horowitz reactor), valorisation, economic support of Haute-Marne and Meuse territories (the Syndiese project), nuclear dismantling and decontamination (dismantling projects, projects and works in Fontenay-aux-Roses, Grenoble and Saclay, waste and material flow management, nuclear service facilities, transports). It also presents the activities of some specific CEA centres like Marcoule (R and D in fuel cycle), Cadarache (future energies) and Saclay (nuclear sciences and simulation of reactors and fuel cycle)

  7. Mapping the antigenic structure of porcine parvovirus at the level of peptides

    DEFF Research Database (Denmark)

    Kamstrup, Søren; Langeveld, Jan; Bøtner, Anette;

    1998-01-01

    The antigenic structure of the capsid proteins of porcine parvovirus (PPV) was investigated. A total of nine linear epitopes were identified by Pepscan using porcine or rabbit anti-PPV antisera. No sites were identified with a panel of neutralising monoclonal antibodies (MAbs). All epitopes were...... located in the region corresponding to the major capsid protein VP2. Based on this information, and on analogy to other autonomous parvoviruses, 24 different peptides were synthesised, coupled to keyhole limpet haemocyanin (KLH) and used to immunise rabbits. Most antisera were able to bind viral protein...

  8. Decontamination and dismantling at the CEA; L'assainissement et le demantelement au CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This document presents the dismantling policy at the CEA (French Research Center on the atomic energy), the financing of the decontamination and the dismantling, the regulatory framework, the knowledge and the technology developed at the CEA, the radiation protection, the environment monitoring and the installations. (A.L.B.)

  9. Carcino-Embryonic Antigen

    International Nuclear Information System (INIS)

    Tumour marker analysis has increased our understanding of the presence of tumours in the body. Carcino-embryonic antigen, CEA, is one of the best studied tumour markers and has proved an ideal diagnostic adjuvant. It has helped in quantifying the amount of disease present in a patient and thence to make accurate prognosis on the various diagnosed ailments. At UCH, it is observed that there is an increase in cancer related ailments and therefore the need for early diagnosis is more compelling in our environment to mitigate future cost of managing advanced manifestation

  10. Plasma TIMP-1 levels and treatment outcome in patients treated with XELOX for metastatic colorectal cancer

    DEFF Research Database (Denmark)

    Frederiksen, C.; Qvortrup, C.; Christensen, I.J.; Glimelius, B.; Berglund, Å.; Jensen, B.V.; Nielsen, S.E.; Keldsen, N.; Nielsen, H.J.; Brunner, Nils; Pfeiffer, P.

    2011-01-01

    associations between baseline TIMP-1 or CEA levels and best response to treatment or progression-free survival (PFS) could be demonstrated. In contrast, high baseline plasma TIMP-1 levels were associated with poor overall survival (OS), P = 0.008, hazard ratio (HR) = 1.80 [95% confidence interval (CI): 1......BACKGROUND: The aim was to evaluate the association between plasma tissue inhibitor of metalloproteinase-1 (TIMP-1) and serum carcinoembryonic antigen (CEA) levels and outcome in patients with metastatic colorectal cancer (mCRC) receiving XELOX (combination chemotherapy with capecitabine and...... oxaliplatin) as first-line treatment. PATIENTS AND METHODS: One hundred and twenty patients were included. Blood samples were collected before treatment and 3 weeks later before the next treatment cycle. Plasma TIMP-1 and serum CEA levels were correlated to treatment outcome. RESULTS: No significant...

  11. Immunoradiometric assay for carcinoembryonic antigen using avidin-biotin separation technique

    International Nuclear Information System (INIS)

    A sensitive, specific, noncompetitive, sandwich-type radioimmunoassay for carcinoembryonic antigen (CEA) has been developed in authors' laboratory, which can be performed conveniently. The assay involves two monoclonal antibodies, selected for high affinity and specificity and also for reaction against antigenic sites on CEA that are distal from each other. One of these antibodies was labelled with 125I and the other was conjugated covalently to biotin. Polystyrene tubes were conjugated covalently to avidin. These tubes represent a rapid, simple method for separating the CEA-bound antibody from the free antibody. The biotin-antibody-CEA-12'5I-labelled antibody complexes bind to the tubes and CEA concentration is directly related to counts per minute. This assay can detect the CEA at a concentration of 0.22 μg/L in serum

  12. Presentation of CEA Marcoule laboratory department

    International Nuclear Information System (INIS)

    The CEA Marcoule Laboratory Department initially dedicated to production support, has recently been renovated to perform a wide range of analyses to support dismantling of plant operation and process development units. It provides services to the operators of the CEA Marcoule Research Center in the fields of analytical chemistry, metallographic examinations, radioactivity measurements, in situ or online nuclear measurements, decontamination processes and industrial chemistry studies. The facilities are designed to analyze all types of nuclear industrial and research samples both under inactive conditions and in glove box and hot cell environments. The Marcoule Laboratory today combines the skills of CEA researchers and AREVA NC analysts in support of production and R and D, process development and dismantling activities at Marcoule or other nuclear sites. (authors)

  13. Development of radiopharmaceuticals based on aptamers: selection and characterization of DNA aptamers for CEA

    Energy Technology Data Exchange (ETDEWEB)

    Correa, C.R.; Andrade, A.S.R., E-mail: antero@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Augusto-Pinto, L. [BioAptus, Belo Horizonte, MG (Brazil); Goes, A.M., E-mail: goes@icb.ufmg.br [Departamento de Imunologia e Bioquimica. Instituto de Ciencias Biologicas. Universidade Federal de Minas Gerais. Belo Horizonte, MG (Brazil)

    2011-07-01

    Colorectal cancer is among the top four causes of cancer deaths worldwide. Carcinoembryonic antigen (CEA) is a complex intracellular glycoprotein produced by about 90% of colorectal cancers. CEA has been identified as an attractive target for cancer research because of its pattern of expression in the surface cell and its likely functional role in tumorigenesis. Research on the rapid selection of ligands based on the SELEX (systematic evolution of ligands by exponential enrichment) forms the basis for the development of high affinity and high specificity molecules, which can bind to surface determinants of tumour cells, like CEA. The oligonucleotides ligands generated in this technique are called aptamers. Aptamers can potentially find applications as therapeutic or diagnostic tools for many kind of diseases, like a tumor. Aptamers offer low immunogenicity, good tumour penetration, rapid uptake and fast systemic clearance, which favour their application as effective vehicles for radiotherapy. In addition aptamers can be labeled with different radioactive isotopes. The aim of this work was select aptamers binding to the CEA tumor marker. The aptamers are obtained through by SELEX, in which aptamers are selected from a library of random sequences of synthetic DNA by repetitive binding of the oligonucleotides to target molecule (CEA). Analyses of the secondary structure of the aptamers were determined using the m fold toll. Three aptamers were selected to binding assay with target cells. These aptamers were confirmed to have affinity and specific binding for T84 cell line (target cell), showed by confocal imaging. We are currently studying the potential efficacy of these aptamers as targeted radiopharmaceuticals, for use as imaging agents or therapeutic applications. The development of aptamers specific to CEA open new perspectives for colorectal cancer diagnosis and treatment. Acknowledgments: This investigation was supported by the Centro de Desenvolvimento da

  14. Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa.

    Science.gov (United States)

    Muenzner, Petra; Kengmo Tchoupa, Arnaud; Klauser, Benedikt; Brunner, Thomas; Putze, Johannes; Dobrindt, Ulrich; Hauck, Christof R

    2016-05-01

    Attachment to the host mucosa is a key step in bacterial pathogenesis. On the apical surface of epithelial cells, members of the human carcinoembryonic antigen (CEA) family are abundant glycoproteins involved in cell-cell adhesion and modulation of cell signaling. Interestingly, several gram-negative bacterial pathogens target these receptors by specialized adhesins. The prototype of a CEACAM-binding pathogen, Neisseria gonorrhoeae, utilizes colony opacity associated (Opa) proteins to engage CEA, as well as the CEA-related cell adhesion molecules CEACAM1 and CEACAM6 on human epithelial cells. By heterologous expression of neisserial Opa proteins in non-pathogenic E. coli we find that the Opa protein-CEA interaction is sufficient to alter gene expression, to increase integrin activity and to promote matrix adhesion of infected cervical carcinoma cells and immortalized vaginal epithelial cells in vitro. These CEA-triggered events translate in suppression of exfoliation and improved colonization of the urogenital tract by Opa protein-expressing E. coli in CEA-transgenic compared to wildtype mice. Interestingly, uropathogenic E. coli expressing an unrelated CEACAM-binding protein of the Afa/Dr adhesin family recapitulate the in vitro and in vivo phenotype. In contrast, an isogenic strain lacking the CEACAM-binding adhesin shows reduced colonization and does not suppress epithelial exfoliation. These results demonstrate that engagement of human CEACAMs by distinct bacterial adhesins is sufficient to blunt exfoliation and to promote host infection. Our findings provide novel insight into mucosal colonization by a common UPEC pathotype and help to explain why human CEACAMs are a preferred epithelial target structure for diverse gram-negative bacteria to establish a foothold on the human mucosa. PMID:27171273

  15. Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa.

    Directory of Open Access Journals (Sweden)

    Petra Muenzner

    2016-05-01

    Full Text Available Attachment to the host mucosa is a key step in bacterial pathogenesis. On the apical surface of epithelial cells, members of the human carcinoembryonic antigen (CEA family are abundant glycoproteins involved in cell-cell adhesion and modulation of cell signaling. Interestingly, several gram-negative bacterial pathogens target these receptors by specialized adhesins. The prototype of a CEACAM-binding pathogen, Neisseria gonorrhoeae, utilizes colony opacity associated (Opa proteins to engage CEA, as well as the CEA-related cell adhesion molecules CEACAM1 and CEACAM6 on human epithelial cells. By heterologous expression of neisserial Opa proteins in non-pathogenic E. coli we find that the Opa protein-CEA interaction is sufficient to alter gene expression, to increase integrin activity and to promote matrix adhesion of infected cervical carcinoma cells and immortalized vaginal epithelial cells in vitro. These CEA-triggered events translate in suppression of exfoliation and improved colonization of the urogenital tract by Opa protein-expressing E. coli in CEA-transgenic compared to wildtype mice. Interestingly, uropathogenic E. coli expressing an unrelated CEACAM-binding protein of the Afa/Dr adhesin family recapitulate the in vitro and in vivo phenotype. In contrast, an isogenic strain lacking the CEACAM-binding adhesin shows reduced colonization and does not suppress epithelial exfoliation. These results demonstrate that engagement of human CEACAMs by distinct bacterial adhesins is sufficient to blunt exfoliation and to promote host infection. Our findings provide novel insight into mucosal colonization by a common UPEC pathotype and help to explain why human CEACAMs are a preferred epithelial target structure for diverse gram-negative bacteria to establish a foothold on the human mucosa.

  16. Development of radiopharmaceuticals based on aptamers: selection and characterization of DNA aptamers for CEA

    International Nuclear Information System (INIS)

    Colorectal cancer is among the top four causes of cancer deaths worldwide. Carcinoembryonic antigen (CEA) is a complex intracellular glycoprotein produced by about 90% of colorectal cancers. CEA has been identified as an attractive target for cancer research because of its pattern of expression in the surface cell and its likely functional role in tumorigenesis. Research on the rapid selection of ligands based on the SELEX (systematic evolution of ligands by exponential enrichment) forms the basis for the development of high affinity and high specificity molecules, which can bind to surface determinants of tumour cells, like CEA. The oligonucleotides ligands generated in this technique are called aptamers. Aptamers can potentially find applications as therapeutic or diagnostic tools for many kind of diseases, like a tumor. Aptamers offer low immunogenicity, good tumour penetration, rapid uptake and fast systemic clearance, which favour their application as effective vehicles for radiotherapy. In addition aptamers can be labeled with different radioactive isotopes. The aim of this work was select aptamers binding to the CEA tumor marker. The aptamers are obtained through by SELEX, in which aptamers are selected from a library of random sequences of synthetic DNA by repetitive binding of the oligonucleotides to target molecule (CEA). Analyses of the secondary structure of the aptamers were determined using the m fold toll. Three aptamers were selected to binding assay with target cells. These aptamers were confirmed to have affinity and specific binding for T84 cell line (target cell), showed by confocal imaging. We are currently studying the potential efficacy of these aptamers as targeted radiopharmaceuticals, for use as imaging agents or therapeutic applications. The development of aptamers specific to CEA open new perspectives for colorectal cancer diagnosis and treatment. Acknowledgments: This investigation was supported by the Centro de Desenvolvimento da

  17. Serum and pancreatic juice carcinoembryonic antigen in pancreatic and biliary disease.

    OpenAIRE

    Carr-Locke, D L

    1980-01-01

    Serum and pancreatic juice carcinoembryonic antigen (CEA) concentrations were studied in a group of 144 patients undergoing endoscopic retrograde cholangiopancreatography (ERCP) with a variety of benign and malignant pancreatic and biliary diseases. Serum CEA was found to be a poor diagnostic and discriminating marker for pancreatic disorders and was raised in obstructive jaundice from various causes correlating with serum alkaline phosphatase. A pancreatic juice CEA concentration of greater ...

  18. An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes

    OpenAIRE

    2012-01-01

    A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl4 − to Au0 o...

  19. Detection of Carcinoembryonic Antigens Using a Surface Plasmon Resonance Biosensor

    Directory of Open Access Journals (Sweden)

    Shin-Ichiro Nishimura

    2008-07-01

    Full Text Available Carcinoembryonic antigen (CEA is an oncofoetal cell-surface glycoprotein that serves as an important tumor marker for colorectal and some other carcinomas. In this work, a CEA immunoassay using a surface plasmon resonance (SPR biosensor has been developed. SPR could provide label-free, real-time detection with high sensitivity, though its ability to detect CEA in human serum was highly dependent on the analytical conditions employed. We investigated the influences of various analytical conditions including immobilization methods for anti-CEA antibody and composition of sensor surface on the selective and sensitive detection of CEA. The results show that anti-CEA antibody immobilized via Protein A or Protein G caused a large increase in the resonance signal upon injection of human serum due to the interactions with IgGs in serum, while direct covalent immobilization of anti-CEA antibody could substantially reduce it. An optimized protocol based on further kinetic analysis and the use of 2nd and 3rd antibodies for the sandwich assay allowed detecting spiked CEA in human serum as low as 25 ng/mL. Furthermore, a self-assembled monolayer of mixed ethylene-glycol terminated alkanethiols on gold was found to have a comparable ability in detecting CEA as CM5 with thick dextran matrix and C1 with short flat layer on gold.

  20. Immunoassay of antigens

    International Nuclear Information System (INIS)

    A method is described of immunoassay of an antigen in a liquid sample wherein a complex is formed between antigen contained in the said sample and two or more antibody reagents, and the said complex is bound to a solid support by non-covalent bonding as defined herein: and the amount of complex becoming bound to the support is determined; the process employing at least one monoclonal antibody reagent. Labelling methods including radioactive, fluorimetric and enzyme labelling may be used to effect determination of the binding ofthe complex to the solid support. The solid support may take the form of particles, beads, wall-coatings on the reaction vessel or an insert of large surface area. The method is particularly applicable to the assay of TSH, CEA, HCG, alphafeto protein, immunoglobulins, viruses, allergens, bacteria, toxins, drugs and vitamins. Use of monoclonal reagents improves the specificity of the process, and also decreases non-specific binding

  1. Press tour Siloe CEA/GRENOBLE

    International Nuclear Information System (INIS)

    The experimental reactor, Siloe, has been stopped the 23 december 1997. This paper of the Cea Grenoble, presents the historical aspects of this reactor and its missions. It gives then a global description of the stopping and dismantling procedure, with the planning, the financing and the human impacts of the operation. The wastes management is also takes into account. (A.L.B.)

  2. CEA, CYFRA 21-1, NSE, and ProGRP in the diagnosis of lung cancer: a multivariate approach

    OpenAIRE

    Gruber, Christine; Hatz, Rudolf; Reinmiedl, Judith; Nagel, Dorothea; Stieber, Petra

    2008-01-01

    We retrospectively studied the single and combined diagnostic value of carcinoembryonic antigen (CEA), cytokeratin fragment 19 (CYFRA 21-1), neuron specific enolase (NSE) and pro-gastrin-releasing peptide (ProGRP), which were routinely analysed in patients with lung tumours of unknown origin at the time of admission to hospital. Inclusion criteria were the determination of CEA (AxSYM/Abbott), CYFRA 21-1 (ElecSys/Roche) and NSE (Kryptor/Brahms). We examined 1747 patients, where 1325 suffered f...

  3. Multiarmed star-like platinum nanowires with multienzyme assembly for direct electronic determination of carcinoembryoninc antigen in serum.

    Science.gov (United States)

    Su, Biling; Tang, Dianping; Tang, Juan; Cui, Yuling; Chen, Guonan

    2011-12-15

    A new electrochemical immunoassay strategy for direct detection of carcinoembryoninc antigen (CEA) in serum was developed by using multiarmed star-like platinum nanowires (PtNWs) with biomolecular assembly as signal tags on an anti-CEA-functionalized graphene sensing platform. Initially, the PtNWs were synthesized via a wet chemical method, and then the synthesized PtNWs were used for the co-immobilization of CEA and horseradish peroxidase (HRP). Compared with platinum nanoparticles, the prepared PtNWs could provide a large room for the conjugation of HRP and CEA. With a competitive-type immunoassay format, the assay was performed in two types of supporting electrolytes including new born cattle serum (NBCS) and acetate buffer solution (ABS, pH 5.5), respectively. Similar detection limit (LOD) of 5.0 pg mL(-1) vs. 1.0 pg mL(-1) but narrower dynamic working linear range of 0.01-60 ng mL(-1) vs. 0.002-80 ng mL(-1) was obtained toward CEA standards in the NBCS compared to the ABS. The intra-assay coefficients of variation (CVs) were 4.3%, 8.6%, and 6.2% at 0.05, 10, and 40 ng mL(-1) CEA, respectively, while the inter-assay CVs were 7.6%, 10.5%, and 8.9% at the above-mentioned levels, respectively. In addition, the selectivity and stability of the electrochemical immunosensor were acceptable. Importantly, the developed method was used to assay clinical serum specimens, receiving a good relation with those obtained from the referenced method. PMID:21982336

  4. Interaction of Proliferating Cell Nuclear Antigen With DNA at the Single Molecule Level

    KAUST Repository

    Raducanu, Vlad-Stefan

    2016-05-01

    Proliferating cell nuclear antigen (PCNA) is a key factor involved in Eukaryotic DNA replication and repair, as well as other cellular pathways. Its importance comes mainly from two aspects: the large numbers of interacting partners and the mechanism of facilitated diffusion along the DNA. The large numbers of interacting partners makes PCNA a necessary factor to consider when studying DNA replication, either in vitro or in vivo. The mechanism of facilitated diffusion along the DNA, i.e. sliding along the duplex, reduces the six degrees of freedom of the molecule, three degrees of freedom of translation and three degrees of freedom of rotation, to only two, translation along the duplex and rotational tracking of the helix. Through this mechanism PCNA can recruit its partner proteins and localize them to the right spot on the DNA, maybe in the right spatial orientation, more effectively and in coordination with other proteins. Passive loading of the closed PCNA ring on the DNA without free ends is a topologically forbidden process. Replication factor C (RFC) uses energy of ATP hydrolysis to mechanically open the PCNA ring and load it on the dsDNA. The first half of the introduction gives overview of PCNA and RFC and the loading mechanism of PCNA on dsDNA. The second half is dedicated to a diffusion model and to an algorithm for analyzing PCNA sliding. PCNA and RFC were successfully purified, simulations and a mean squared displacement analysis algorithm were run and showed good stability and experimental PCNA sliding data was analyzed and led to parameters similar to the ones in literature.

  5. Fecal Calprotectin levels and Serological Responses to Microbial Antigens among Children and Adolescents with Inflammatory Bowel Disease

    Science.gov (United States)

    Ashorn, Sara; Honkanen, Teemu; Kolho, Kaija-Leena; Ashorn, Merja; Välineva, Tuuli; Wei, Bo; Braun, Jonathan; Rantala, Immo; Luukkaala, Tiina; Iltanen, Sari

    2008-01-01

    Objectives Non-invasive, sensitive and specific tools for early identification of chronic inflammatory bowel diseases (IBD) are needed for clinical practice. The aim was to identify new non-invasive test combinations for characterization of IBD in children and adolescents by comparing serological responses to microbial antigens and fecal calprotectin, a new promising marker for intestinal inflammation. Patients and methods Our study included 73 children who underwent endoscopies because of suspicion of IBD. Their sera were tested for antibodies to the Pseudomonas fluorescens-associated sequence I2, a Bacteroides caccae TonB-linked outer membrane protein, OmpW and anti-Saccharomyces cerevisiae (ASCA). Simultaneously, samples for fecal calprotectin measurements were obtained from 55 subjects. Results IBD was diagnosed in 60 patients (CD in 18 patients, UC in 36 and IC in six). Thirteen children had a non-IBD disease. Fecal calprotectin levels were elevated (≥ 100ug/g) more frequently in IBD patients (89%, 39/44) compared to non-IBD cases (9%, 1/11, p<0.001). ASCA antibodies in sera were detected in 67% (12/18) of patients with CD, in 14% (5/36) of the children with UC and in 50% (3/6) of patients with IC. Seroreactivity for I2 was observed in 42% of the IBD patients, this frequency being higher than in non-IBD cases (7,7% seropositive; p=0.025). Serum anti-I2 IgA levels (median absorbances) were higher in those with IBD compared to those without gut inflammation (p=0.039). The combination of the measurements of fecal calprotectin and serological responses to microbial antigens (ASCA, I2 and OmpW) identified 100% of CD patients (sensitivity 100%, specificity 36%, PPV 66%, NPV 100%) and 89% of UC patients (sensitivity 89%, specificity 36%, PPV 77%, NPV 57%). Conclusions Increased levels of serological responses to microbial antigens (ASCA, I2 and OmpW) and fecal calprotectin are evident in both CD and UC patients. The combination of these markers provides valuable

  6. 1999 scientific evaluation at the CEA; L'evaluation scientifique 1999 au CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-07-01

    This report presents a statement of the scientific and technical activity of the French atomic energy commission (CEA) for the year 1999. This evaluation is made by external and independent experts and requires some specific dispositions for the nuclear protection and safety institute (IPSN) and for the direction of military applications (DAM). The report is divided into 4 parts dealing successively with: 1)the CEA, a public research organization (strategy, research programs, new organization of the CEA activities, civil nuclear research, technology research and transfer, defence activities, transfer of knowledge) 2)the scientific evaluation at the CEA (evaluations of the civil applications of the CEA, IPSN, DAM, INSTN (national institute for nuclear sciences and techniques) 3)synthesis of the 1999 scientific and technical evaluation for each operational directions of the CEA (directions of fuel cycle, of nuclear reactors, of advanced technologies, of materials sciences, of life sciences, of military applications, of the nuclear protection and safety institute and of the national institute for nuclear sciences and techniques) 4)the corresponding members of the evaluation and the list of scientific and technical councils and members.

  7. CEA: place and role in national science and industry

    International Nuclear Information System (INIS)

    The data on the main trends in CEA activity are given. The role, which CEA plays in French scientific and engineering spheres, is estimated. Examples of CEA international links are discussed, and its contribution into the World scientific and technical progress is shown

  8. Technical Aspect of Shielded SIMS Installation in CEA Cadarache

    International Nuclear Information System (INIS)

    A shielded IMS 6f has been installed in the LECA facility, CEA Cadarache France. The nuclearization was performed by CAMECA Company, which sells the standard IMS 6f. Working on nuclear materials requires in depth modifications of the apparatus itself. Despite these modifications, the shielded SIMS has the same level of performance as the standard apparatus. The design of the modified apparatus is presented and the safety aspects are emphasised. The shielded SIMS should be allowed to handle irradiated samples at the end of 2001. (Author)

  9. Distinct patterns of blood-stage parasite antigens detected by plasma IgG subclasses from individuals with different level of exposure to Plasmodium falciparum infections

    Directory of Open Access Journals (Sweden)

    Højrup Peter

    2010-10-01

    Full Text Available Abstract Background In endemic regions naturally acquired immunity against Plasmodium falciparum develops as a function of age and exposure to parasite infections and is known to be mediated by IgG. The targets of protective antibodies remain to be fully defined. Several immunoepidemiological studies have indicated an association of cytophilic anti-parasite IgG with protection against malaria. It has been hypothesized that the initial antibody responses against parasite antigens upon first few Plasmodium falciparum infections is dominated by non-protective IgG2/IgG4 and IgM antibodies, which then gradually develop into protective response dominated by cytophilic IgG1 and IgG3 antibodies. Methods Naturally occurring IgG antibodies against P. falciparum blood-stage antigens were analysed from plasma samples collected from four groups of individuals differing in age and level of exposure to P. falciparum infections. Western Blot profiling of blood-stage parasite antigens displaying reactivity with individual plasma samples in terms of their subclass specificities was conducted. Parasite antigens detected by IgG were grouped based on their apparent molecular sizes resolved by SDS-PAGE as high molecular weight (≥ 70 kDa or low molecular weight (P. falciparum infections. Results IgG4 and IgM antibodies in plasma samples from all groups detected very few parasite antigens. IgG2 antibodies from all groups detected a common pattern of high molecular weight parasite antigens. Cytophilic IgG subclasses in plasma samples from individuals with higher levels of exposure to P. falciparum infections distinctly detected higher numbers of low molecular weight parasite antigens. Conclusions In the present study, there was no evidence for switching of antibody responses from non-cytophilic to cytophilic subclasses against blood-stage parasite antigens as a likely mechanism for induction of protective immunity against malaria.

  10. Situation 2002: release monitoring and surveillance of environment of Cea centers; Bilan 2002: controle des rejets et surveillance de l'environnement des centres CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    This publication renders an account of the situation of the releases of liquid and gaseous radioactive effluents, for the year 2002, as well as the radioactivity levels measured in the vicinity of Cea centers through the systematic surveillance of atmosphere, waters, vegetation and milk. An analysis on five years allows to follow their evolution. (N.C.)

  11. Elevated serum levels of fetal antigen 1,a member of the epidermal growth factor superfamily, in patients with small cell lung cancer

    DEFF Research Database (Denmark)

    Harken Jensen, C; Drivsholm, L; Laursen, I; Teisner, B

    1999-01-01

    Serum levels of fetal antigen 1 (FA1) were quantified pretherapeutically in 16 patients with pneumonia, 30 patients with small cell lung cancer (SCLC) and 10 patients with non-small cell lung cancer (NSCLC) and compared to the normal reference interval (n = 177). Serum FA1 levels were significantly...

  12. Bone scans in conjunction with prostata-specific antigen levels in patients with prostate cancer

    International Nuclear Information System (INIS)

    The usefulness of the determination of PSA for primary diagnosis, follow-up, and therapy-monitoring of prostatic cancer is undebatable. It has been shown that more than 50% of the patients with stage A and B and about 90% of the patients with stage D demonstrate PSA-levels above the cut-off-level (4.0 ng/ml). Detection of bone metastases by bonescans is regarded as a marker for an extensive disease. In this stage, numerous groups have reported on PSA-levels >20 ng/ml in more than 98% of the patients. For PSA-levels 10 ng/ml or in cases of locally advanced stages with highly undifferentiated tumors. For follow-up purposes, the use of bonescans should be restrictes to patients with PSA-levels >20 ng/ml. Nevertheless, a bone-scan is mandatory for the evaluation of bone-pain, before surgery for local recurrence and before local radiation therapy. (orig.)

  13. Content of the canceroembryonal antigen in tumours of the organs of the abdominal cavity and the retroperitoneal space

    International Nuclear Information System (INIS)

    The concentration of the canceroembryonal antigen (CEA) has been determined in vitro by the radioimmunological analysis of the blood plasma of 70 donors and 130 patients with malignant tumours of the intestine (6 persons), stomach (77), with relapsing gastric carcinoma (12), tumours of the pancreas (7), retroperitoneal space (18) and with inflammatory disease of the gastrointestinal tract (12). It is established that the radioimmunological test in vitro for CEA can be used not only for primary diagnosis of cancer of the rectum but also as a substantial supplement to the clinical diagnosis of carcinoma of the stomach and the pancreas. A relationship revealed between the rise in the content of CEA and the extent of malignancy provides additional information for correct diagnosing the stage of gastric carcinoma and a choice of an adequate method of treatment. Tolerance of the CEA level in the blood in retroperitoneal tumours and inflammatory diseases of the gastrointestinal tract may serve as a criterion for differential diagnosis of malignant newgrowths in the abdominal cavity

  14. Observation on CEA and IL-6 contents in gastric juice

    International Nuclear Information System (INIS)

    Objective: To study the changes of CEA and IL-6 contents in blood and gastric juice in patients with gastric cancer and gastritis. Methods: CEA and IL-6 contents in blood and gastric juice were measured with RIA in 60 patients and 30 controls. Results: Gastric juice CEA and IL-6 contents in patients with gastric carcinoma were significantly higher than those in the controls (p < 0.001), however, CEA and IL-6 contents in patients with gastritis and controls were not much different. Conclusion: Gastric juice CEA and IL-6 assay is of diagnostic significance in patients with gastric malignant tumor

  15. CEA/Valduc Plutonium Recycling Facility Project

    International Nuclear Information System (INIS)

    Pu recycling activities are operated in the 118 facility on Valduc CEA/DAM center. This facility, built in 1963, does not meet modern safety requirements especially for fire and earthquake hazards. The last safety review commission in 2000 gave the authorization to continue recycling operations in the 118 facility until the end of 2010 with the obligation of the achievement, of a safety upgrade program in 2005. At the same time, CEA/DAM has decided to launch the project for the building of a new facility for Pu recycling and legacy residues stabilization. In addition to specific requirements for Pu metal preparation, the processes retained for this facility have to optimize safety, wastes, costs and dosimetry. Moreover, all wastes have to be suitable for discard or of for a safe long term storage in nuclear vault. First conceptual design elements and important basic safety requirements will be presented in this communication. (authors)

  16. The CEA contribution to quality control

    International Nuclear Information System (INIS)

    The Commissariat a l'Energie Atomique has developed original methods and techniques for testing delicate components of the primary circuit of pressure water reactors. These techniques make use of a very wide range of non destructive testing methods: Eddy currents, particularly multiple frequencies, for testing steam generator tubes, gudgeon and other pins focused ultrasonics for testing all the welds of the reactor vessel and its cover plate, mixed welds of steam vessels and generators, low welds of the pressurizer and gudgeon pins from the inside. On site use is effected with specific machines intended either for inspecting the tube bundles of steam generators under the responsibility of INTERCONTROLE Co., or for the complete examination of the reactor vessel by mixed CEA/INTERCONTROLE crews under the responsibility of the CEA. All these operations are subjected to a programme of quality assurance that provides the guaranty of execution complying with the procedures in force

  17. When environment specialists meet the CEA

    International Nuclear Information System (INIS)

    The Afite, the French association of environment engineers and technicians, has organized in May 26 and 27, 1997 a visit of the Cadarache and Marcoule CEA's laboratories devoted to environmental research. The CEA (the French Atomic Energy Commission) and its associated companies represent about ten research centres in which original techniques are developed in order to solve some environmental problems related to nuclear and non-nuclear activities. This paper presents the main environmental research activities carried out at the Cadarache and Marcoule centres: climate evolution and global environment, seismic risks, industrial risks, plants/environment interactions (removal of pollutants, hydrocarbons and heavy metals from the soil), radioactive wastes characterization and processing, cycles of pollutants, nuclear fuels enrichment (SILVA process), remote handling techniques, spent fuels reprocessing, confinement of nuclear wastes and dismantling of nuclear installations. (J.S.)

  18. Serum levels of ficolin-3 (Hakata antigen) in patients with systemic lupus erythematosus

    DEFF Research Database (Denmark)

    Andersen, T.; Munthe-Fog, L.; Garred, P.; Andersen, Trine; Munthe-Fog, Lea; Garred, Peter; Jacobsen, Søren

    2009-01-01

    OBJECTIVE: Ficolin-3 is a serum protein of putative importance in autoimmunity. Our objective was to investigate any differential expression of ficolin-3 in patients with systemic lupus erythematosus (SLE) or its clinical subsets. METHODS: Serum levels of ficolin-3 (S-ficolin-3) were determined in...

  19. CEA: assessment of risk management 2011

    International Nuclear Information System (INIS)

    This report proposes an overview of CEA activities in the field of risk management in different areas: impact on the environment, installation safety, management of occupational risks (occupational health and safety), radiological protection of workers, transportation of hazardous materials, waste management, protection of sites, installations and heritage, management of emergency situations, management of law risks, controls and audits. It finally presents the risk management department

  20. Levels of Renibacterium salmoninarum antigens in resident and anadromous salmonids in the River Ellidaár system in Iceland.

    Science.gov (United States)

    Kristmundsson, Á; Árnason, F; Gudmundsdóttir, S; Antonsson, T

    2016-06-01

    In relation to stock enhancement programmes, wild salmon broodfish have been routinely screened for the presence of Renibacterium salmoninarum antigens (Rs-Ag) for decades. A sudden increase in the prevalence of Rs-Ag experienced caused extensive problems to this industry as eggs from positive fish are discarded. The prevalence and level of Rs-Ag were examined in resident and anadromous salmonids in the River Ellidaár system and the progress of Rs-Ag in a cohort of salmon followed. Both prevalence and Rs-Ag levels were high in resident salmonids and emigrating salmon smolts in the river system. When the smolts re-entered their home river as adults the following summer, they were almost free of Rs-Ag, but the longer they stayed in the river, the more Rs-Ag they acquired; the majority being positive at spawning. This study demonstrates a high level of Rs-Ag in salmonids in the River Ellidaár system which significantly reduces in the salmon during its seawater phase. Accordingly, it seems ideal to sample salmon broodfish as soon as possible after ascending the river and subsequently transfer to Rs-free environment for storage until stripping, which could result in lower Rs-prevalence and minimize the problems that stock enhancement programmes have faced due to Rs-positive wild broodfish. PMID:26275672

  1. Structural Integrity Analysis of CEA Change Platform

    International Nuclear Information System (INIS)

    The Control Element Assembly Change Platform (CEA CP) is similar to a gantry crane. The CEA CP for Shin-Kori units 3 and 4 (SKN 3 and 4) consists of a bridge, which spans the reactor cavity pool and a gantry superstructure mounted on the bridge. The structure is approximately 8.8 m wide, 4.9 m long and 10.6 m high. The gantry superstructure supports one ton capacity hoist trolley and the bridge supports the In Core Instrumentation (ICI) retrieval cart which moves along the bridge. This paper presents the dynamic and structural analysis of CEA CP which is greater than that of the previous nuclear power plants to verify the structural integrity under the application of the earthquake spectrum. The analysis have been performed using the three orthogonal SSE response spectrum for SKN 3 and 4 which shows much higher acceleration value than OPR- 1000 Plants. In addition, the analyses are performed by 3-dimensional finite element analysis using ANSYS software

  2. Carcinoembryonic antigen is anchored to membranes by covalent attachment to a glycosylphosphatidylinositol moiety: identification of the ethanolamine linkage site.

    OpenAIRE

    Hefta, S A; Hefta, L J; Lee, T.D. (Taunia D.); Paxton, R J; Shively, J. E.

    1988-01-01

    The COOH-terminal amino acid of carcinoembryonic antigen (CEA) is shown to covalently link with ethanolamine, evidence consistent with the anchorage of CEA to the plasma membrane through a phosphatidylinositol-glycan tail. Purified CEA was digested with trypsin, and the resulting peptides were isolated by reverse-phase HPLC. Tryptic hexapeptide T12, terminating atypically with alanine, corresponded in sequence (Ser-Ile-Thr-Val-Ser-Ala) with the last six residues (637-642) of the third repeati...

  3. Effects of experimental immunosuppression in cattle with persistently high antibody levels to Salmonella Dublin lipopolysaccharide O-antigens

    Directory of Open Access Journals (Sweden)

    Nielsen Liza R

    2007-08-01

    Full Text Available Abstract Background Salmonella Dublin (S. Dublin is a zoonotic bacterium which is host adapted to cattle. The bacterium can cause subclinical persistent infection in cattle (carriers, which may be reactivated. During reactivation, animals may shed bacteria, thus constituting a source of infection for other animals. Identification of such carriers is assumed to be critical in attempts to control and eradicate the infection. Some authors suggest that persistently high antibody levels in serum or milk is indicative of a carrier state in cattle. However, this has been questioned by other studies in which S. Dublin were not found in all animals suspected of being carriers based on antibody measurements when such animals were examined at slaughter. Some hypothesize that the lack of isolated bacteria from long-term high antibody level cattle is due to a latent infection stage that can later be reactivated, for instance during stress around calving or due to transportation. This study examined nine adult cattle with persistently high antibody responses to S. Dublin O-antigen based lipopolysaccharide for cultivable bacteria in faeces, milk and internal organs before and after transportation, isolation and experimental immunosuppression with dexamethasone sodium phosphate over a period of 7–14 days. Results Clear signs of immunosuppression were seen as expression of leucocytosis and neutrophilia in all animals on day 3–5 after the first injections with dexamethasone sodium phosphate. No clinical signs or necropsy findings indicating salmonellosis were observed in any of the animals. No shedding of S. Dublin was found in faeces (collected four times daily or milk (collected twice daily at any point in time during the 7–14 day period. S. Dublin was recovered by a conventional culture method from tissue samples from mammary lymph nodes, spleen and liver collected from three animals at necropsy. Conclusion In this study, immunosuppression by

  4. Immunoglobulin Concentrations and Antigen-Specific Antibody Levels in Cervicovaginal Lavages of Rhesus Macaques Are Influenced by the Stage of the Menstrual Cycle

    OpenAIRE

    Lü, F. Xusheng; Ma, Zhongmin; Rourke, Tracy; Srinivasan, Seema; McChesney, Michael; Christopher J. Miller

    1999-01-01

    The levels of antigen-specific antibodies (Abs) and immunoglobulins in the cervical mucus of women vary with the menstrual cycle; the highest levels occur during menses, and the lowest occur during the periovulatory period. The rhesus macaque is a widely used animal model of female genital tract immunity. We sought to determine whether rhesus macaques have a cyclical pattern of changing cervicovaginal Ab and immunoglobulin levels that is similar to that of the human female. ...

  5. Utility of squamous cell carcinoma antigen, carcinoembryonic antigen, Cyfra 21-1 and neuron specific enolase in lung cancer diagnosis: a prospective study from China

    Institute of Scientific and Technical Information of China (English)

    SONG Wei-an; LIU Xi; TIAN Xiao-dong; WANG Wei; LIANG Chao-yang; ZHANG Tao; GUO Jun-tang; PENG Yang-hong; ZHOU Nai-kang

    2011-01-01

    Background Early detection and diagnosis is urgent for the sake of effective treatment strategy for lung cancer.However,a convenient,economical and relatively precise method is not available.We here report a prospective study to find the possible value of the combined use of four popular tumor markers in the early diagnosis of lung cancer among patients with suspicious nodules in the lung.Methods Six hundred and sixty inpatients with suspicious nodules in the lung were divided into a lung cancer group and a benign pulmonary tumor group according to post-operative histological examinations.Serum levels of four tumor markers including squamous cell carcinoma antigen (SCC),carcinoembryonic antigen (CEA),Cyfra 21-1 and neuron specific enolase (NSE) were assayed for each patient.Receiver operating characteristic (ROC) curves were constructed for each tumor marker.The power of lung cancer diagnosis of each tumor marker,as well as a combination of them were analyzed and compared.Results The serum levels (median,range) of SCC,CEA,Cyfra 21-1 and NSE were 0.44 (0.01-35.70) ng/ml,2.49(0.30-26.78) ng/ml,2.30 (0.82-73.33) ng/ml and 10.54 (0.10-56.41) ng/ml respectively in lung cancer group,and were 0.32 (0.01-0.90) ng/ml,1.60 (0.20-8.93) ng/ml,1.41 (0.72-4.82) ng/ml and 9.36 (6.56-24.24) ng/ml respectively in the benign pulmonary tumor group.The difference in each tumor marker between the two groups was significant (P <0.05).The ROCs of SCC,CEA,Cyfra 21-1 and NSE were 0.702 (95% CI,0.654-0.751),0.611 (95% CI,0.563-0.659),0.650(95% CI,0.601-0.700) and 0.598 (95% CI,0.542-0.654) respectively,indicating very low power of these four tumor markers.When a combination of SCC,CEA,Cyfra 21-1 and NSE were employed,the diagnosis power was strengthened.Conclusion SCC,CEA,Cyfra 21-1 and NSE are valuable in the early diagnosis of lung cancer among suspicious nodules in the lung,especially when they were assayed together for one patient.

  6. Report transparency and nuclear safety 2007 CEA Grenoble; Rapport transparence et securite nucleaire 2007 CEA Grenoble

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Grenoble for the year 2007. Since 2002 the Passage project aims to realize the decontamination and the dismantling of old nuclear installations of the CEA Grenoble. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the year 2007 saw two main steps of the Passage project: the decommissioning of the Siloette reactor, a public consultation about the Lama laboratory dismantling. (A.L.B.)

  7. Updated on effluents releases of the CEA nuclear fuel cycle facilities - 1995 to 2010 period

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Nelson Luiz Dias [Centro Tecnologico da Marinha em Sao Paulo (CTMSP) Sao Paulo, SP (Brazil)

    2011-07-01

    The environmental impact assessment of the Centro Experimental Aramar (CEA) facilities has been presented in a former work, based on the measured effluent releases data, for the period from 1995 to 2007. This work shows the update up to 2010. The effluents releases to the environment result from the routine operation of CEA nuclear fuel cycle facilities (LEI - Isotopic Enrichment Laboratory, USIDE - Pilot Plant for Industrial Verification of Uranium Enrichment and LABMAT - Nuclear Materials Laboratory). Basically, this work presents the radioactive release source terms, as described at the CEA Effluent Report sent to the National Commission for Nuclear Energy (CNEN) each semester, and a historical assessment of the critical group annual doses from 1995 up to 2010. The assessed doses are compared to the maximum dose constraint as well as to the exemption level specified by CNEN. (author)

  8. Evaluation of tumor markers carcinoembryonic antigen, cytokeratin 19 fragment and cancer-associated antigen 72-4 in neoplastic and non-neoplastic canine effusions differentiation

    Directory of Open Access Journals (Sweden)

    L.V. Teixeira

    2014-10-01

    Full Text Available The concentration of tumor markers in body fluids can be used for diagnosis and prognosis of patients. This study aimed to investigate the performance of tumor markers cytokeratin 19 fragment (CYFRA 21-1, cancer-associated antigen 72-4 (CA 72-4 and carcinoembryonic antigen (CEA in the neoplastic and non-neoplastic canine effusions. In thirty-two neoplastic (n=16 and non-neoplastic (n=16 samples of canine thoracic or abdominal effusions, tumor markers were measured. Significant statistical difference was found only for the CYFRA 21-1 marker. The levels were significantly higher for the neoplastic group. The lack of significance between groups for markers CA 72-4 and CEA can be explained by the presence of other diseases in the non-neoplastic group, causing elevated levels of these markers. This study concludes that CYFRA 21-1 performed well, showing good sensitivity, specificity and accuracy in the diagnosis of neoplastic effusions in dogs. However, further investigations are necessary in patients with malignancy as those with benign effusions.

  9. The releases control and the environment survey of the Cea Centers

    International Nuclear Information System (INIS)

    The Cea sets the environment protection in the heart of its security policy, which is based on the mastership of risks resulting from the researches activities and installations development. This policy aims to reduce as small as possible compared with the technical and economical necessities, the impact of its activities on the human and the environment. This document, takes stock for the year 2002, of the liquid and gas radioactive effluents releases as also of the radioactivity level around the Cea Centers, by a systematic monitoring of the atmosphere, the waters, the vegetation and the milk. (A.L.B.)

  10. Induction of Anti-Tumor Immunity Ex Vivo Using Dendritic Cells Transduced with Fowl Pox Vector Expressing MUC1, CEA, and a Triad of Costimulatory Molecules (rF-PANVAC)1

    Science.gov (United States)

    Vasir, Baldev; Zarwan, Corrine; Ahmad, Rehan; Crawford, Keith D; Rajabi, Hassan; Matsuoka, Ken-Ichi; Rosenblatt, Jacalyn; Wu, Zekui; Mills, Heidi; Kufe, Donald; Avigan, David

    2012-01-01

    The fowl pox vector expressing the tumor associated antigens MUC1 and CEA in the context of costimulatory molecules (rF-PANVAC) has shown promise as a tumor vaccine. However, vaccine mediated expansion of suppressor T cell populations may blunt clinical efficacy. We characterized the cellular immune response induced by ex-vivo dendritic cells (DCs) transduced with (rF)-PANVAC. Consistent with the functional characteristics of potent antigen presenting cells, rF-PANVAC-DCs demonstrated strong expression of MUC1 and CEA and costimulatory molecules, CD80, CD86, and CD83; decreased levels of phosphorylated STAT3, and increased levels of Tyk2, JAK2 and STAT1. rF-PANVAC-DCs stimulated expansion of tumor antigen specific T cells with potent cytolytic capacity. However, rF-PANVAC transduced DCs also induced the concurrent expansion of FOXP3 expressing CD4+CD25+high regulatory T cells (Tregs) that inhibited T cell activation. Moreover, Tregs expressed high levels of Th2 cytokines (IL-10, IL-4, IL-5, and IL-13) together with phosphorylated STAT3 and STAT6. In contrast, the vaccine expanded Treg population expressed high levels of Th1 cytokines IL-2 and IFNγ and the proinflammatory RORγt and IL-17A suggesting that these cells may share effector functions with conventional TH17 T cells. These data suggest that Tregs expanded by rF-PANVAC-DCs, exhibit immunosuppressive properties potentially mediated by Th2 cytokines, but simultaneous expression of Th1 and Th17 associated factors suggests a high degree of plasticity. PMID:22892452

  11. Charting a course through the CEAs: diagnosis and management of medullary thyroid cancer.

    Science.gov (United States)

    Rowe, Christopher W; Bendinelli, Cino; McGrath, Shaun

    2016-09-01

    Medullary thyroid cancer (MTC) is an uncommon thyroid cancer that requires a high index of suspicion to facilitate diagnosis of early-stage disease amenable to surgical cure. The challenges of diagnosis, as well as management in the setting of persistent disease, are explored in the context of a case presenting with the incidental finding of elevated carcinoembryonic antigen (CEA) and an (18) F-fluorodeoxyglucose positron emission tomography ((18) F-FDG-PET)-positive thyroid incidentaloma detected following treatment of colorectal cancer. Strategies to individualize prognosis, and emerging PET-based imaging modalities, particularly the potential role of (18) F-DOPA-PET in staging, are reviewed. PMID:27230389

  12. Elevated serum levels of a biliary glycoprotein (BGP I) in patients with liver or biliary tract disease

    International Nuclear Information System (INIS)

    Human hepatic bile contains a glycoprotein (Biliary glycoprotein I BGP I) which cross-reacts with the carcinoembryonic antigen (CEA). A radioimmunoassay for BGP I was developed. The interference of CEA or 'non-specific cross-reacting antigen' (NCA) was small. The serum levels of BGP I were determined in healthy subjects, in patients with hepato-biliary diseases and in patients with various infections or inflammatory disorders. Healthy individuals, including pregnant women, had a serum BGP I concentration of about 0.5 - 1 mg/l. Diseases of the liver or biliary tract (e.g. hepatitis A or B, cytomegalovirus Hepatitis, obstructive jaundice or primary biliary cirrhosis) were associated with elevated serum levels of BGP I, as opposed to infectious diseases not affecting the liver mostly showing values within the normal range. Raised levels of serum BGP I activity may reflect biliary obstruction as a results of interference with normal BGP I secretion in the bile. (author)

  13. HIV-1 Adaptation to Antigen Processing Results in Population-Level Immune Evasion and Affects Subtype Diversification

    Directory of Open Access Journals (Sweden)

    Stefan Tenzer

    2014-04-01

    Full Text Available The recent HIV-1 vaccine failures highlight the need to better understand virus-host interactions. One key question is why CD8+ T cell responses to two HIV-Gag regions are uniquely associated with delayed disease progression only in patients expressing a few rare HLA class I variants when these regions encode epitopes presented by ∼30 more common HLA variants. By combining epitope processing and computational analyses of the two HIV subtypes responsible for ∼60% of worldwide infections, we identified a hitherto unrecognized adaptation to the antigen-processing machinery through substitutions at subtype-specific motifs. Multiple HLA variants presenting epitopes situated next to a given subtype-specific motif drive selection at this subtype-specific position, and epitope abundances correlate inversely with the HLA frequency distribution in affected populations. This adaptation reflects the sum of intrapatient adaptations, is predictable, facilitates viral subtype diversification, and increases global HIV diversity. Because low epitope abundance is associated with infrequent and weak T cell responses, this most likely results in both population-level immune evasion and inadequate responses in most people vaccinated with natural HIV-1 sequence constructs. Our results suggest that artificial sequence modifications at subtype-specific positions in vitro could refocus and reverse the poor immunogenicity of HIV proteins.

  14. Benign Hydronephrosis and Elevated of Serum Levels of Carbohydrate Antigen CA 19-9: A Case Report.

    Science.gov (United States)

    Filipovic, Branka; Milinić, Nikola; Gacic, Jasna; Markovic, Olivera; Djokovic, Aleksandra; Filipovic, Branislav

    2016-01-01

    BACKGROUND Carbohydrate tumor-associated antigen (CA 19-9) has been shown to be upregulated in other malignant tumors including gastric, ovarian, hepatocellular, and colorectal carcinoma as well as benign diseases of the biliary track such as pancreatitis, cholangitis, and choledocholithiasis. According to the available literature, in several cases of benign hydronephrosis and in a few cases of benign renal diseases, elevated CA 19-9 has been noted. CASE REPORT A 58-year-old Caucasian male patient was admitted in our clinic with complaints about blunt abdominal pain in the past two-month period localized in the right lumbar region and irradiating into the right inguinal area, constipation, abdominal bloating, and intermittent hematuria. The concentration of serum CA 19-9 was 3500 U/mL. Urine cytology provided no signs of abnormality. Intravenous urography visualized right-sided pyelon and ureter duplex with the defect in contrast shade of the pyelon, caused by a stag horn calculus. Contrast added computerized axial tomography of the abdomen and pelvis visualized the pyelon casted concretion spreading throughout the right pyelon, with ureterohydronephrosis with the distal block for passage of the contrast to the distal part of the ureter. CONCLUSIONS There is no doubt that CA 19-9 level is occasionally elevated in patients with obstructive urolithiasis as it was in our case. In the routine medical praxis, urolithiasis should not be neglected in the differential diagnosis of elevated concentrations of CA 19-9 marker. PMID:27287959

  15. Proposta para estadiamento do câncer colorretal baseada em critérios morfofuncionais: correlação com níveis séricos do antígeno carcinoembrionário Proposal for colorectal cancer stages based on morphofunctional criteria: correlation with carcinoembryonic antigen levels

    Directory of Open Access Journals (Sweden)

    Denise Gonçalves Priolli

    2007-12-01

    tempo de sobrevida, a classificação morfofuncional e o nível sérico de antígeno carcinoembrionário. CONCLUSÃO: O estadiamento morfofuncional é válido para a avaliação prognóstica dos pacientes com adenocarcinoma colorretal, e relaciona-se com os níveis séricos do CEA.The analysis of morphofunctions characteristics can be useful in the colorectal cancer evolution, especially if related to the serum carcinoembryonic antigen levels. The research of chromosomes and genes instability, as well as the alterations of tissue protein codified, makes attractive the possibility to use potentially valid functional factors as variables for the understanding of colorectal carcinoma prognosis. OBJECTIVE: To consider classes based on morphologic and functional colorectal carcinoma characteristics, valuing serum carcinoembryonic antigen levels prognostic power. METHOD: Third-five patients in different stages of colorectal carcinoma underwent operations from 2001 to 2007. Serum CEA levels, histological grade, tissue CEA cell polarization capacity were analyzed. Colorectal carcinoma was classified according to TNM stages. The morphofunctional classification was determined by the combination between histological grade and antigen polarization, morphofunctional stages have been based in association between morphofunctional classification and stages TNM, by punctuation attributed to each one classification. The results had been analyzed by variance analysis, correlation test and survival analysis (Kaplan-Meier and Cox Model Regression, adopting p<0.05. RESULTS: Morphofunctional stages survival curve resulted similar to the joined ones in stages TNM. It had relation between new classification proposed and patient survival time. They had observed relation among survival time, morphofunctional classification and serum carcinoembryonic antigen. CONCLUSION: Morphofunctional classification is valid for colorectal cancer patient's prognostic evaluation and is related with the serum CEA

  16. Status of Cea spallation modules for ads

    International Nuclear Information System (INIS)

    In the framework of CEA studies on ADS dedicated to waste transmutation, a liquid metal reference concept and an alternative solid target have been evaluated to produce neutrons inside the spallation module. This work examines the design (neutronic, thermohydraulic and mechanical aspects) and the performances of both options. It is shown that a liquid Pb-Bi target offers more possibilities regarding to high protons current densities (possible industrial extrapolation) but that a solid target made with tungsten particles offers also interesting ability to create a neutrons flux appropriated (strong spectrum and flat axial distribution) to an sub-critical core dedicated to incineration. (author)

  17. Algunas orquidáceas americanas

    OpenAIRE

    Williams, Louis O.

    2012-01-01

    La presente contribución al conocimiento de las Orquidáceas americanas trata de algunas especies de México, América Central, Sur América y las Antillas. Estas notas son el resultado de determinaciones de varios ejemplares colectados en tales regiones, y que se hallan depositados en el Ames Herbarium, Cambridge, Mass., el United States National Museum, Washington, D. C., el New York Botanical Garden Herbarium, Nueva York, o en el Herbarium of the Missouri Botanical Garden, St.Louis, Missouri....

  18. Radionuclide-Based Cancer Imaging Targeting the Carcinoembryonic Antigen

    Directory of Open Access Journals (Sweden)

    Hao Hong

    2008-01-01

    Full Text Available Carcinoembryonic antigen (CEA, highly expressed in many cancer types, is an important target for cancer diagnosis and therapy. Radionuclide-based imaging techniques (gamma camera, single photon emission computed tomography [SPECT] and positron emission tomography [PET] have been extensively explored for CEA-targeted cancer imaging both preclinically and clinically. Briefly, these studies can be divided into three major categories: antibody-based, antibody fragment-based and pretargeted imaging. Radiolabeled anti-CEA antibodies, reported the earliest among the three categories, typically gave suboptimal tumor contrast due to the prolonged circulation life time of intact antibodies. Subsequently, a number of engineered anti-CEA antibody fragments (e.g. Fab’, scFv, minibody, diabody and scFv-Fc have been labeled with a variety of radioisotopes for CEA imaging, many of which have entered clinical investigation. CEA-Scan (a 99mTc-labeled anti-CEA Fab’ fragment has already been approved by the United States Food and Drug Administration for cancer imaging. Meanwhile, pretargeting strategies have also been developed for CEA imaging which can give much better tumor contrast than the other two methods, if the system is designed properly. In this review article, we will summarize the current state-of-the-art of radionuclide-based cancer imaging targeting CEA. Generally, isotopes with short half-lives (e.g. 18F and 99mTc are more suitable for labeling small engineered antibody fragments while the isotopes with longer half-lives (e.g. 123I and 111In are needed for antibody labeling to match its relatively long circulation half-life. With further improvement in tumor targeting efficacy and radiolabeling strategies, novel CEA-targeted agents may play an important role in cancer patient management, paving the way to “personalized medicine”.

  19. Situation 2002: release monitoring and surveillance of environment of Cea centers

    International Nuclear Information System (INIS)

    This publication renders an account of the situation of the releases of liquid and gaseous radioactive effluents, for the year 2002, as well as the radioactivity levels measured in the vicinity of Cea centers through the systematic surveillance of atmosphere, waters, vegetation and milk. An analysis on five years allows to follow their evolution. (N.C.)

  20. Plasma TIMP-1 and CEA as markers for detection of primary colorectal cancer

    DEFF Research Database (Denmark)

    Christensen, Ib Jarle; Brünner, Nils; Dowell, Barry;

    2015-01-01

    endoscopy were prospectively included (N=1965). Baseline data and co-morbidity were recorded. The primary end-point was the detection of CRC. Plasma was obtained before endoscopy and TIMP-1 and CEA levels were determined using an automated analysis platform when all samples were collected. RESULTS: CRC was...

  1. 188Re-IorCEA1 as radioimmunotherapeutical agent: Preparation and evaluation

    International Nuclear Information System (INIS)

    Effective immunotherapy of human malignancies requires the selective delivery of these agents to relevant antigen-expressing tumour cells. A variety of strategies have been investigated to achieve this objective. It has very clearly established that ior-CEA1 monoclonal antibody is an extremely good agent for immunoscintigraphy with a high sensitivity (94%) and specificity (87%) (OLIVA 94, OLIVA 95). Ior-CEA1 is hence a good candidate for development as a therapeutic agent. Moreover, it is an attractive novel protein, which conserves its biological properties, despite chemical treatment. Radiolabelling of ior-CEA1 is accomplished by a direct method using 2-mercaptoethanol as the reducing agent. Two different weak chelating agents were employed, which had been reported in other published works before. All experiments carried out with MDP showed low labelling yields and high precipitate formation, even while SnF2, which has better redox properties. The best labelling yields were attained with low citric acid concentration, as well as with 400 μg of SnF2 at pH 3. The optimised method gave high labelling yields (∼ 95 %). The best labelling yields were attained with low citric acid concentration, which was used as a weak transchelation ligand. Ascorbic acid and Albumin could be used as the stabilising agents in order to achieve less than 3,5% dissociation

  2. Serum levels of vascular endothelial growth factor and cancer antigen 125 are related to the prognosis of adenomyosis patients after interventional therapy

    OpenAIRE

    MU, YONGXU; Hu, Xiaoyan; He, Junfeng; Liu, Haiyan; Zhang, Lei; Liu, Heming; Hao, Zhiming

    2015-01-01

    Aims: This study is to investigate the expression levels of vascular endothelial growth factor (VEGF) and cancer antigen 125 (CA125) in serum of adenomyosis patients before and after interventional therapy. The role of serum levels of VEGF and CA125 for the prognosis of adenomyosis is further studied. Methods: A total of 80 adenomyosis patients treated with interventional therapy and 40 healthy individuals were enrolled in this study. Enzyme-linked immunosorbent assay was performed to detect ...

  3. An Information Building on Radioactivity and Nuclear Energy for the French CEA Cadarache Research Center - 13492

    International Nuclear Information System (INIS)

    The CEA Cadarache research center is one of the 10 research centers of the French Alternative Energies and Atomic Energy Commission (CEA). Distributed throughout various research platforms, it focuses on nuclear fission, nuclear fusion, new energy technologies (hydrogen, solar, biomass) and fundamental research in the field of vegetal biology. It is the most important technological research and development centers for energy in Europe. Considering the sensitive nature of nuclear activities, the questions surrounding the issue of radioactive waste, the nuclear energy and the social, economic and environmental concerns for present and future generations, the French Government asked nuclear actors to open communication and to give all the information asked by the Local Information Commission (CLI) and the public [1]. In this context, the CEA Cadarache has decided to better show and explain its expertise and experience in the area of nuclear energy and nuclear power plant design, and to make it available to stakeholders and to the public. CEA Cadarache receives each year more than 9000 visitors. To complete technical visits of the research facilities and laboratories, a scientific cultural center has been built in 2011 to inform the public on CEA Cadarache research activities and to facilitate the acceptance of nuclear energy in a way suited to the level of knowledge of the visitors. A modern interactive exhibition of 150 m2 allows visitors to find out more about energy, CEA Cadarache research programs, radioactive waste management and radiological impact on the research center activities. It also offers an auditorium for group discussions and for school groups to discover science through enjoyment. This communication center has received several thousand visitors since its opening on October 2011; the initial results of this experience are now available. It's possible to explain the design of this exhibition, to give some statistics on the number of the visitors, their

  4. Successful Antiparasitic Treatment for Cysticercosis is Associated with a Fast and Marked Reduction of Circulating Antigen Levels in a Naturally Infected Pig Model.

    Science.gov (United States)

    Gonzalez, Armando E; Bustos, Javier A; Garcia, Hector H; Rodriguez, Silvia; Zimic, Mirko; Castillo, Yesenia; Praet, Nicolas; Gabriël, Sarah; Gilman, Robert H; Dorny, Pierre

    2015-12-01

    Taenia solium cysticercosis is a common parasitic infection of humans and pigs. We evaluated the posttreatment evolution of circulating parasite-specific antigen titers in 693 consecutive blood samples from 50 naturally infected cysticercotic pigs, which received different regimes of antiparasitic drugs (N = 39, 7 groups), prednisone (N = 5), or controls (N = 6). Samples were collected from baseline to week 10 after treatment, when pigs were euthanized and carefully dissected at necropsy. Antigen levels decreased proportionally to the efficacy of treatment and correlated with the remaining viable cysts at necropsy (Pearson's p = 0.67, P = 0.000). A decrease of 5 times in antigen levels (logarithmic scale) compared with baseline was found in 20/26 pigs free of cysts at necropsy, compared with 1/24 of those who had persisting viable cysts (odds ratio [OR] = 76.7, 95% confidence interval [CI] = 8.1-3308.6, P < 0.001). Antigen monitoring reflects the course of infection in the pig. If a similar correlation exists in infected humans, this assay may provide a minimally invasive and easy monitoring assay to assess disease evolution and efficacy of antiparasitic treatment in human neurocysticercosis. PMID:26392159

  5. Progress in fusion reactors blanket analysis and evaluation at CEA

    International Nuclear Information System (INIS)

    In the frame of the recent CEA studies aiming at the development, evaluation and comparison of solid breeder blanket concepts in view of their adaptation to NET, and the evaluation of specific questions related to the first wall design, the present paper examines first the performances of a helium cooled toroidal blanket design for NET, based on innovative Beryllium/Ceramics breeder rod elements. Neutronic and thermo-mechanical optimisation converges on a concept featured by a breeding capability in excess of 1.2, a reasonnable pumping power of 1 % and a narrow breeder temperature range (470 +- 30 0C of the breeder), the latter being largely independent of the power level. The final section of the paper is devoted to the evaluation of the heat load poloidal distribution and to the irradiation effects on first wall structural materials

  6. Simplifying CEA through Excel, VBA, and Subeq

    Science.gov (United States)

    Foster, Ryan

    2004-01-01

    Many people use compound equilibrium programs for very different reasons, varying from refrigerators to light bulbs to rockets. A commonly used equilibrium program is CEA. CEA can take various inputs such as pressure, temperature, and volume along with numerous reactants and run them through equilibrium equations to obtain valuable output information, including products formed and their relative amounts. A little over a year ago, Bonnie McBride created the program subeq with the goal to simplify the calling of CEA. Subeq was also designed to be called by other programs, including Excel, through the use of Visual Basic for Applications (VBA). The largest advantage of using Excel is that it allows the user to input the information in a colorful and user-friendly environment while allowing VBA to run subeq, which is in the form of a FORTRAN DLL (Dynamic Link Library). Calling subeq in this form makes it much faster than if it were converted to VBA. Since subeq requires such large lists of reactant and product names, all of which can't be passed in as an array, subeq had to be changed to accept very long strings of reactants and products. To pass this string and adjust the transfer of input and output parameters, the subeq DLL had to be changed. One program that does this is Compaq Visual FORTRAN, which allows DLLs to be edited, debugged, and compiled. Compaq Visual FORTRAN uses FORTRAN 90/95, which has additional features to that of FORTRAN 77. My goals this summer include finishing up the excel spreadsheet of subeq, which I started last summer, and putting it on the Internet so that others can use it without having to download my spreadsheet. To finish up the spreadsheet I will need to work on debugging current options and problems. I will also work on making it as robust as possible, so that all errors that may arise will be clearly communicated to the user. New features will be added old ones will be changed as I receive comments from people using the spreadsheet

  7. Prognostic Value of Pretreatment Carcinoembryonic Antigen After Definitive Radiotherapy With or Without Concurrent Chemotherapy for Squamous Cell Carcinoma of the Uterine Cervix

    International Nuclear Information System (INIS)

    Purpose: To evaluate whether pretreatment carcinoembryonic antigen (CEA) levels have a prognostic role in patients after definitive radiotherapy for squamous cell carcinoma (SCC) of the uterine cervix. Methods and Materials: A retrospective study of 550 patients was performed. The SCC antigen (SCC-Ag) and CEA levels were regarded as elevated when they were ≥2 and ≥5 ng/mL, respectively. A total of 208 patients underwent concurrent chemoradiotherapy (CCRT). The Kaplan-Meier method was used to calculate the distant metastasis (DM), local failure (LF), disease-free survival (DFS), and overall survival (OS) rates. Multivariate analysis was performed using the Cox proportional hazards model. The hazard ratio (HR) with 95% confidence interval (CI) was evaluated for the risk of a poor prognosis. Results: Compared with the patients with normal CEA/SCC-Ag levels, CEA levels ≥10 ng/mL but without elevated SCC-Ag levels was an independent factor for LF (HR, 51.81; 95% CI, 11.51–233.23; p < .001), DM (HR, 6.04; 95% CI, 1.58–23.01; p = .008), DFS (HR, 10.17; 95% CI, 3.18–32.56; p < .001), and OS (HR, 5.75; 95% CI, 1.82–18.18; p = .003) after RT alone. However, no significant role for CEA was noted in patients with SCC-Ag levels ≥2 ng/mL. In patients undergoing CCRT, a CEA level ≥10 ng/mL was an independent factor for LF (HR, 2.50; 95% CI, 1.01–6.21; p = .047), DM (HR, 3.41; 95% CI, 1.56–7.46; p = .002), DFS (HR, 2.73; 95% CI, 1.39–5.36; p = .003), and OS (HR, 3.93; 95% CI 1.99–7.75; p < .001). A SCC-Ag level of ≥40 ng/mL was another prognostic factor for DM, DFS, and OS in patients undergoing not only CCRT, but also RT alone. The 5-year OS rate for CCRT patients with CEA <10 ng/mL and ≥10 ng/mL was 75.3% and 35.8%, respectively (p < .001). CCRT was an independent factor for better OS (HR, 0.69; 95% CI, 0.50–0.97; p = .034). Conclusion: Pretreatment CEA levels in patients with SCC of the uterine cervix provide complementary information for

  8. Superconducting quadrupoles for LHC : CERN/CEA-CEN Saclay Collaboration

    CERN Multimedia

    CEA Saclay and CERN Collaboration

    1994-01-01

    Agreement has been signed between CERN and CEA Saclay, concerning the construction of two prototypes of the LHC latest quadrupoles. CEA Saclay was interested with the study, the design, the construction and the testing of this magnet. The fabrication took place at the CEN Laboratory at Saclay in Paris.

  9. Low carbon energies: action by the CEA

    International Nuclear Information System (INIS)

    In future decades we can expect the situation regarding energy and climate change to become more and more difficult. On one hand, population growth and essential development in emerging economies will increase the demand for energy; on the other, the depletion of fossil fuel reserves, procurement difficulties due to geopolitical tension, and global warming are all making the search for viable and competitive alternatives to hydrocarbons all the more urgent. In the 2007 edition of its World Energy Outlook report, the International Energy Agency (IEA) made the position clear. CEA is one of the major European research institutes working in the field of energy, he is part of European Energy Research Alliance (EERA). (authors)

  10. The Grenoble CEA Center: dismantled and rehabilitated

    International Nuclear Information System (INIS)

    The denuclearization program of the CEA center in Grenoble was launched in 2001. It involves 6 nuclear facilities (3 research reactors: Melusine, Siloette, and Siloe, and 1 laboratory (LAMA) and 2 units for processing wastes). The dismantling works were finished at the end of 2012 and the 2013 program concerns: the demolition of the buildings homing Melusine and Siloe reactors, the final rehabilitation of the Siloe raft, and the final rehabilitation of the laboratory and of the waste processing units. The budget is 117*106 euros for Siloe, 28*106 euros for Melusine, 6*106 euros for Siloette, 70*106 euros for the LAMA, and 90*106 euros for the 2 waste processing units. (A.C.)

  11. The complementary safety assessments of CEA's facilities

    International Nuclear Information System (INIS)

    The safety assessments of CEA's facilities was made in 2 steps. The first step that was performed end 2011 involved facilities that had priority according to the French Safety Authority among them the Jules Horowitz reactor being built in Cadarache, the Masurca reactor (being upgraded in Cadarache), the Plutonium workshop (being dismantled in Cadarache), the Osiris reactor (that operates in Saclay) and the Phenix reactor (decommissioned at Marcoule). The second step was performed in 2012. The main conclusion is the setting of a hard core of measures concerning equipment and organization that is able to sustain the vital functions of each facility in any accidental situation. Another consequence is the reinforcement of the emergency means (equipment and organization) in the Cadarache site. (A.C.)

  12. CEA programme on gas cooled reactors

    International Nuclear Information System (INIS)

    Future nuclear energy systems studies conducted by the CEA aim at investigating and developing promising technologies for future reactors, fuels and fuel cycles, for nuclear power to play a major part in sustainable energy policies. Reactors and fuel cycles are considered as integral parts of a nuclear system to be optimised as a whole. Major goals assigned to future nuclear energy systems are the following: reinforced economic competitiveness with other electricity generation means, with a special emphasis on reducing the investment cost; enhanced reliability and safety, through an improved management of reactor operation in normal and abnormal plant conditions; minimum production of long lived radioactive waste; resource saving through an effective and flexible use of the available resources of fissile and fertile materials; enhanced resistance to proliferation risks. The three latter goals are essential for the sustainability of nuclear energy in the long term. Additional considerations such as the potentialities for other applications than electricity generation (co-generation, production of hydrogen, sea water desalination) take on an increasing importance. Sustainability goals call for fast neutron spectra (to transmute nuclear waste and to breed fertile fuel) and for recycling actinides from the spent fuel (plutonium and minor actinides). New applications and economic competitiveness call for high temperature technologies (850 deg C), that afford high conversion efficiencies and hence less radioactive waste production and discharged heat. These orientations call for breakthroughs beyond light water reactors. Therefore, as a result of a screening review of candidate technologies, the CEA has selected an innovative concept of high temperature gas cooled reactor with a fast neutron spectrum, robust refractory fuel, direct conversion with a gas turbine, and integrated on-site fuel cycle as a promising system for a sustainable energy development. This objective

  13. CEA experimental feedback on sodium loop decommissioning

    International Nuclear Information System (INIS)

    The aim of this paper is to present experimental feedback on sodium loop dismantling techniques at the CEA (The French Atomic Energy Commission) and to offer recommendations for the decommissioning of Fast Reactor secondary sodium loops. This study is based on acquired CEA decommissioning experience which primarily concerns the following: the decommissioning of RAPSODIE (France's first Fast Reactor), the PHENIX reactor secondary loop replacement, the sodium loop decommissioning carried out by the Laboratory of Sodium Technologies and Treatment, and several technical documents. This paper deals with the main results of this survey. First, a comparison of 8 pipe-cutting techniques is made, taking into account speed in cutting, reliability, dissemination, fire risk due to the presence of sodium, cutting depth, and different types of waste (empty pipes, sodium-filled pipes, tanks...). This comparison has led us to recommend the use of an alternative saw or a chain saw rather than the use of the plasma torch or grinder. Different techniques are recommended depending on if they are on-site, initial cuttings or if they are to be carried out in a specially-designed facility referred to hereafter as 'the cutting building'. After the cutting stage, the sodium waste must be processed with water to become an ultimate stable waste. Four treatment processes are compared with different standards : speed, cost, low activity adaptability and 'large sodium quantity' adaptability. Recommendations are also made for reliable storage, and for the general dismantling system organization. Last, calculations are presented concerning a complete dismantling facility prototype capable of treating large amounts and volume of sodium wastes. (author)

  14. Overview of the long-lived radionuclide separation processes developed in connection with the CEA`s spin programme

    Energy Technology Data Exchange (ETDEWEB)

    Madic, C.; Bourges, J.; Dozol, J.F.

    1994-12-31

    One possible strategy for eliminating the long-term potential hazards associated with the storage of vitrified high level waste produced by the processing of irradiated nuclear fuels, is the transmutation of the long-life radionuclides in these wastes into short-life radionuclides by nuclear means, which requires the prior chemical separation of these long-life radionuclides from the nuclear waste mixture. To do this, the French CEA (Atomic Energy Commissionership) launched the SPIN (Separation-Incineration) research programme in 1991, under the law passed by the parliament on december 30, 1991. Research currently under way to develop separation processes for some long-life radionuclides concerns modifications of the Purex process, and the development of new solvent extraction processes. The research directions are briefly summarized here. (authors). 17 refs., 3 tabs.

  15. Transcription Activity of Ectogenic Human Carcinoembryonic Antigen Promoter in Lung Adenocarcinoma Cells A549

    Institute of Scientific and Technical Information of China (English)

    XIONG Weining; FANG Huijuan; XU Yongjian; XIONG Shendao; CAO Yong; SONG Qingfeng; ZENG Daxiong; ZHANG Huilan

    2006-01-01

    The transcription activity of ectogenic human carcinoembryonic antigen (CEA) promoter in lung adenocarcinoma cells A549 was investigated for the further gene-targeting therapy. The reporter gene green fluorescent protein (GFP) driven by CEA promoter and human cytomegalovirus (CMV) promoter were relatively constructed and named plasmid pCEA-EGFP and pCMV-GFP respectively. The intensity of fluorescence was detected by fluorescence microscope and flow cytometry analysis after the pCEA-GFP and pSNAV-GFP plasmids were transfected into A549 cells through liposome respectively. The results showed (4.08±0.63) % of the A549 cells transfected with pCEA-AFP plasmid expressed, significantly lower than that of the A549 cells transfected with pCMV-GFP [(43.27±3.54) %]. It was suggested that ectogenic human CEA promoter in lung adenocarcinoma cells A549 was weakly expressed. The distinct specificity of CEA promoter in CEA high expression cells was regarded as a tool in selective gene therapy, but the transcription activity of ectogenic human CEA promoter was needed to increase in the future.

  16. Report transparency and nuclear safety 2007 CEA Saclay; Rapport transparence et securite nucleaire 2007 CEA Saclay

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Saclay for the year 2007. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially two public consultation on release authorizations and the Neurospin installations, the dismantling of the 49 nuclear installation, the shutdown of the learning reactor ULYSSE are detailed. (A.L.B.)

  17. Report transparency and nuclear safety 2007 CEA Marcoule; Rapport transparence et securite nucleaire 2007 CEA Marcoule

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Marcoule for the year 2007. Since its creation in 1955 the center realizes industrial and scientific activities relative to the civil and military applications of the radioactivity. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the following two base activities are detailed: Atalante and Phenix. (A.L.B.)

  18. Report transparency and nuclear safety 2007 CEA Cadarache; Rapport transparence et securite nucleaire 2007 CEA Cadarache

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Cadarache for the year 2007. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the report discusses the beginning of the RJH reactor construction, the fourth generation reactors research programs, the implementing of la Rotonde the new radioactive wastes management installation, the renovation of the LECA. (A.L.B.)

  19. Clinical significance of CYFRA21-1 and CEA in the early prognosis of patients with locally advanced non-small cell lung cancer%CYFRA21-1和CEA对局部晚期非小细胞肺癌患者早期预后的意义

    Institute of Scientific and Technical Information of China (English)

    张丽娜; 么建立; 郝殿晋; 李洪臣; 王术艺; 张庆波

    2013-01-01

    Objective: To investigate the value of evaluation indicators of carcinoembryonic antigen (CEA) and cy-tokeratin 19 fragment( CYFRA21 - 1) as short - term efficacy in patients with advanced non - small cell lung cancer (NSCLC). Methods: The level of serum CEA and CYFRA21 - 1 were detected by electrochemoluminescence immu-noassay in 85 patients with locally advanced non - small cell lung cancer. All statistical analyses were done with software of SPSS14.0. Results:The level of CYFRA21 - 1 and CEA was associated with the therapeutic effect. The level of CEA and CYFRA21 - 1 of patients achieving partial remission (PR) or complete remission ( CR) after treatment were decreased significantly; On the contrary, the level of CEA and CYFRA21 - 1 of patients achieving SD or PD were basically stable or increased. In a multivariate analysis , the level of CEA and CYFRA21 - 1 after one cycle of chemotherapy were confirmed as independent prognostic factors for therapeutic effect. Conclusion; The level of CY-FRA21 - 1 and CEA are associated with the therapeutic effect. Patients may get better prognosis with the decreasing of serum tumor marker level. The level of CEA and CYFRA21 - 1 after one cycle of chemotherapy are confirmed as independent prognostic factors for therapeutic effect.%目的:研究癌胚抗原(CEA)和细胞角蛋白19片段(CYFRA21-1)作为晚期非小细胞肺癌(NSCLC)短期疗效评价指标的价值.方法:采用电化学发光法测定85例初治的局部晚期NSCLC患者化疗前后血清中CEA、CYFRA21-1的水平,所有统计应用SPSS14.0统计软件进行分析.结果:化疗前CEA、CYFRA21-1在治疗有效组(CR+ PR)和无效组(SD+ PD)的分布无显著性差异.二者的水平在治疗有效组的化疗后明显低于化疗前,比较有显著性差异;在无效组化疗后的表达水平高于化疗前,但比较无显著性差异.多因素分析显示化疗前低水平的血清CEA、CYFRA21-1是近期疗效的保护性因素,化疗

  20. Immune-scintigraphy of recurrent colorectal tumors with Tc-99m-marked monoclonal carcinoembryonic antigen antibodies

    International Nuclear Information System (INIS)

    After surgery in 24 patients aged 39-80 years with colorectal tumors, immune-scintigraphic examinations with Tc-99m carcinoembryonic antigen (CEA) MAb were carried out. Prior to scintigraphy, the levels of CEA and Ca 19-9 tumor markers were determined. In the majority of cases there was firm clinical evidence of local recurrence or metastases. Twenty-two of the 24 cases were scintigraphically positive. Local recurrence and metastases of the liver were correctly identified in 12 and four cases, respectively. It was difficult to detect widespread metastases of the peritoneum or a growth at the helum of the lung. In three cases, metastases of lymph nodes were confirmed by both surgery and scintigraphy, although the computed tomographic (CT) examinations were negative. In 20 patients, CT and immune-scintigraphic findings correlated. Most positive scans were confirmed surgically and at biopsy. In two patients, none of the diagnostic procedures showed positive results, despite clinical grounds for suspecting a further spread of the disease. A false-positive immune-scintigram was observed in a histologically confirmed case of inflammation of the mucous membrane. In several cases Tc-99m enhancement was detected in almost all parts of the colon. This could be due to the antibody metabolism or to the deposit of free technetium in the bowel. There was no correlation between CEA serum levels and a positive immune-scan

  1. Mutational Profiles Reveal an Aberrant TGF-β-CEA Regulated Pathway in Colon Adenomas

    Science.gov (United States)

    Jogunoori, Wilma; Menon, Vipin; Majumdar, Avijit; Chen, Jiun-Sheng; Gi, Young Jin; Jeong, Yun Seong; Phan, Liem; Belkin, Mitchell; Gu, Shoujun; Kundra, Suchin; Mistry, Nipun A.; Zhang, Jianping; Su, Xiaoping; Li, Shulin; Lin, Sue-Hwa; Javle, Milind; McMurray, John S.; Rahlfs, Thomas F.; Mishra, Bibhuti; White, Jon; Rashid, Asif; Beauchemin, Nicole; Weston, Brian R.; Shafi, Mehnaz A.; Stroehlein, John R.; Davila, Marta; Akbani, Rehan; Weinstein, John N.; Wu, Xifeng; Mishra, Lopa

    2016-01-01

    Mutational processes and signatures that drive early tumorigenesis are centrally important for early cancer prevention. Yet, to date, biomarkers and risk factors for polyps (adenomas) that inordinately and rapidly develop into colon cancer remain poorly defined. Here, we describe surprisingly high mutational profiles through whole-genome sequence (WGS) analysis in 2 of 4 pairs of benign colorectal adenoma tissue samples. Unsupervised hierarchical clustered transcriptomic analysis of a further 7 pairs of adenomas reveals distinct mutational signatures regardless of adenoma size. Transitional single nucleotide substitutions of C:G>T:A predominate in the adenoma mutational spectrum. Strikingly, we observe mutations in the TGF-β pathway and CEA-associated genes in 4 out of 11 adenomas, overlapping with the Wnt pathway. Immunohistochemical labeling reveals a nearly 5-fold increase in CEA levels in 23% of adenoma samples with a concomitant loss of TGF-β signaling. We also define a functional role by which the CEA B3 domain interacts with TGFBR1, potentially inactivating the tumor suppressor function of TGF-β signaling. Our study uncovers diverse mutational processes underlying the transition from early adenoma to cancer. This has broad implications for biomarker-driven targeting of CEA/TGF-β in high-risk adenomas and may lead to early detection of aggressive adenoma to CRC progression. PMID:27100181

  2. Selection of DMA aptamer that specific binding human carcinoembryonic antigen in vitro

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Objective:To select the specific aptamer of carcinoembryonic antigen (CEA), one of the most attractive molecule for cancer target therapy and imaging. Methods: Seven rounds in vitro selection were performed against the purified CEA protein. Ligand-mediated target purification and Co-immunoprecipitation were adopted to verify the specific binding of the aptamer to the purified and native protein separately. Results:The CEA-specific aptamer which can bind both the purified and native protein with the high specificity was obtained. Conclusion:This is the first time the CEA specific apatmer was produced. The results in this study provides the preliminary evidence for further investigation and application of CEA-aptamer in the future.

  3. Dispersion of Radionuclides and Exposure Assessment in Urban Environments: A Joint CEA and LLNL Report

    Energy Technology Data Exchange (ETDEWEB)

    Glascoe, Lee [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Gowardhan, Akshay [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Lennox, Kristin [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Simpson, Matthew [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Yu, Kristen [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Armand, Patrick [Alternative Energies and Atomic Energy Commission (CEA), Paris (France); Duchenne, Christophe [Alternative Energies and Atomic Energy Commission (CEA), Paris (France); Mariotte, Frederic [Alternative Energies and Atomic Energy Commission (CEA), Paris (France); Pectorin, Xavier [Alternative Energies and Atomic Energy Commission (CEA), Paris (France)

    2014-12-19

    In the interest of promoting the international exchange of technical expertise, the US Department of Energy’s Office of Emergency Operations (NA-40) and the French Commissariat à l'Energie Atomique et aux énergies alternatives (CEA) requested that the National Atmospheric Release Advisory Center (NARAC) of Lawrence Livermore National Laboratory (LLNL) in Livermore, California host a joint table top exercise with experts in emergency management and atmospheric transport modeling. In this table top exercise, LLNL and CEA compared each other’s flow and dispersion models. The goal of the comparison is to facilitate the exchange of knowledge, capabilities, and practices, and to demonstrate the utility of modeling dispersal at different levels of computational fidelity. Two modeling approaches were examined, a regional scale modeling approach, appropriate for simple terrain and/or very large releases, and an urban scale modeling approach, appropriate for small releases in a city environment. This report is a summary of LLNL and CEA modeling efforts from this exercise. Two different types of LLNL and CEA models were employed in the analysis: urban-scale models (Aeolus CFD at LLNL/NARAC and Parallel- Micro-SWIFT-SPRAY, PMSS, at CEA) for analysis of a 5,000 Ci radiological release and Lagrangian Particle Dispersion Models (LODI at LLNL/NARAC and PSPRAY at CEA) for analysis of a much larger (500,000 Ci) regional radiological release. Two densely-populated urban locations were chosen: Chicago with its high-rise skyline and gridded street network and Paris with its more consistent, lower building height and complex unaligned street network. Each location was considered under early summer daytime and nighttime conditions. Different levels of fidelity were chosen for each scale: (1) lower fidelity mass-consistent diagnostic, intermediate fidelity Navier-Stokes RANS models, and higher fidelity Navier-Stokes LES for urban-scale analysis, and (2) lower-fidelity single

  4. RHAMM (CD168) Is Overexpressed at the Protein Level and May Constitute an Immunogenic Antigen in Advanced Prostate Cancer Disease

    OpenAIRE

    Kilian M. Gust; Hofer, Matthias D; Sven R. Perner; Robert Kim; Arul M. Chinnaiyan; Sooryanarayana Varambally; Peter Moller; Ludwig Rinnab; Rubin, Mark A; Jochen Greiner; Michael Schmitt; Rainer Kuefer; Mark Ringhoffer

    2009-01-01

    Localized prostate cancer (CaP) can be cured using several strategies. However, the need to identify active substances in advanced tumor stages is tremendous, as the outcome in such cases is still disappointing. One approach is to deliver human tumor antigen-targeted therapy, which is recognized by T cells or antibodies. We used data mining of the Cancer Immunome Database (CID), which comprises potential immunologic targets identified by serological screening of expression libraries. Candidat...

  5. Antibodies to the enterobacterial common antigen: standardization of the passive hemagglutination test and levels in normal human sera.

    OpenAIRE

    Malkamäki, M

    1981-01-01

    The passive hemagglutination test for antibodies against the enterobacterial common antigen (ECA) of Kunin was standardized for diagnostic purposes. Human erythrocytes were coated with a soluble ECA+ preparation from Salmonella typhimurium or, as specificity controls, with a similar ECA- preparation from congenic ECA-negative bacteria with saline, and the hemagglutination assay was performed on microtiter plates. The specificity of the test was ascertained further by inhibition assays with pu...

  6. Changing of expression level of fas-antigen (CD95), cytokines synthesis and production after irradiation in low doses

    International Nuclear Information System (INIS)

    It is known that bone marrow progenitor (CD34+), tymocytes and peripheral blood lymphocytes (PBL) are most radiosensitive than other cell types. Even low doses of radiation induce apoptosis. The investigators suggest that it is possible relationship between synthesis and production of cytokines and apoptotic process. With the purpose to determine correlation between expression of Fas-antigen and synthesis of cytokines after low doses irradiation the experiments by irradiation PBL of healthy persons in vitro were held. Cells were X-irradiated by 12,5, 25 and 50 cGy. In consequence of the experiments increasing of Fas-antigen was revealed. This increasing correlated with changing in synthesis and production of cytokines. Also the Chernobyl's accident liquidators (CAL) were investigated. After comparison data in the group CAL (I) with data in the control group (II) increasing of Fas-antigen expression was revealed. Also in I group was discovered increasing of the cell number sinthesied interleukine-4 (IL-4) and interleukine-6 (IL-6). Interleukine-lβ (IL-1 β) producing pell were decreased. These changes have been correlated with degree of immunodeficiency at CAL. These data allow to consider the apoptosis as cell mechanism included in pathogenesis of diseases, which can be showed later long time after irradiation. (author)

  7. A Carcinoembryonic Antigen-Secreting Adenocarcinoma Arising in Tailgut Cyst : Clinical Implications of Carcinoembryonic Antigen

    OpenAIRE

    Cho, Byoung Chul; Kim, Nam Kyu; Lim, Beom Jin; Kang, Sang Ook; Sohn, Ju Hyuk; Roh, Jae Kyung; Choi, Sang Tae; Kim, Sung Ai; Park, Se Eun

    2005-01-01

    Tailgut cysts (TGCs) are rare congenital cysts that occur in the retrorectal or presacral spaces. Although most tailgut cysts have been reported as benign, there have been at least 9 cases associated with malignant change. We report herein on an unusual case of a 40-year-old woman with a carcinoembryonic antigen (CEA)-producing adenocarcinoma arising within a TGC who underwent surgical resection and local radiation therapy. Despite the complete resection, metastatic adenocarcinoma developed f...

  8. Detection of carcinoembryonic antigen mRNA in peritoneal washes from gastric cancer patients and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    Yan-Song Zhang; Jun Xu; Guang-Hua Luo; Rong-Chao Wang; Jiang Zhu; Xiao-Ying Zhang; Peter Nilsson-Ehle; Ning Xu

    2006-01-01

    AIM: To establish a more sensitive method for detection of free cancer cells in peritoneal washes from gastric cancer patients during surgery and to evaluate its clinical significance.METHODS: The carcinoembryonic antigen (CEA) mRNA levels in peritoneal washes from 65 cases of gastric cancer were detected by real-time RT-PCR. Peritoneal lavage cytology (PLC) was applied simultaneously to detection of free cancer cells. Negative controls included peritoneal washes from 5 cases of benign gastric disease and blood samples from 5 adult healthy volunteers.RESULTS: There was no CEA mRNA in peritoneal washes from benign gastric disease patients and in blood of adult healthy volunteers. The positive percentage of free cancer cells detected by real-time RT-PCR was 47.7% and only 12.3% by PLC. The positive rate of CEA mRNA was significantly related with serosa invasion between peritoneal metastasis and stage of gastric cancer.CONCLUSION: Real-time RT-PCR is a sensitive and rapid method for the detection of free cancer cells in peritoneal washes. The presence of free cancer cells in peritoneal washes is related to the pathologic stage of gastric cancer.

  9. Antibody levels to multiple malaria vaccine candidate antigens in relation to clinical malaria episodes in children in the Kasena-Nankana district of Northern Ghana

    Directory of Open Access Journals (Sweden)

    Oduro Abraham R

    2011-05-01

    Full Text Available Abstract Background Considering the natural history of malaria of continued susceptibility to infection and episodes of illness that decline in frequency and severity over time, studies which attempt to relate immune response to protection must be longitudinal and have clearly specified definitions of immune status. Putative vaccines are expected to protect against infection, mild or severe disease or reduce transmission, but so far it has not been easy to clearly establish what constitutes protective immunity or how this develops naturally, especially among the affected target groups. The present study was done in under six year old children to identify malaria antigens which induce antibodies that correlate with protection from Plasmodium falciparum malaria. Methods In this longitudinal study, the multiplex assay was used to measure IgG antibody levels to 10 malaria antigens (GLURP R0, GLURP R2, MSP3 FVO, AMA1 FVO, AMA1 LR32, AMA1 3D7, MSP1 3D7, MSP1 FVO, LSA-1and EBA175RII in 325 children aged 1 to 6 years in the Kassena Nankana district of northern Ghana. The antigen specific antibody levels were then related to the risk of clinical malaria over the ensuing year using a negative binomial regression model. Results IgG levels generally increased with age. The risk of clinical malaria decreased with increasing antibody levels. Except for FMPOII-LSA, (p = 0.05, higher IgG levels were associated with reduced risk of clinical malaria (defined as axillary temperature ≥37.5°C and parasitaemia of ≥5000 parasites/ul blood in a univariate analysis, upon correcting for the confounding effect of age. However, in a combined multiple regression analysis, only IgG levels to MSP1-3D7 (Incidence rate ratio = 0.84, [95% C.I.= 0.73, 0.97, P = 0.02] and AMA1 3D7 (IRR = 0.84 [95% C.I.= 0.74, 0.96, P = 0.01] were associated with a reduced risk of clinical malaria over one year of morbidity surveillance. Conclusion The data from this study support the view

  10. Clinical value of CEA and CYFRA 21-1 for the differentiation of benign and malignant pleural effusion by Meta analysis

    International Nuclear Information System (INIS)

    Objective: Pleural effusion is a common clinical complication caused by many benign or malignant diseases. Many published papers presented that tumor-marker assays, such as carcinoembryonic antigen (CEA) and cytokeratin 19 fragment antigen 21-1 (CYFRA 21-1), played a vital role on the benign and malignant differentiation. This study aimed to reviewe currently available published papers to evaluate the clinical value of these two markers for the differentiation of benign and malignant pleural effusion. Methods: All published data of pleural effusion CEA and CYFRA 21-1 for the differentiation studies from year 1996 to 2007 were reviewed. By methodological qualities and Q test, 19 papers were selected into this study. The overall diagnostic sensitivity, specificity, and area under curve (AUC) from receiver operating characteristic (ROC) curve were adopted to compare the value of pleural effusion CEA and CYFRA 21-1 for benign and malignant differentiation used Z test. Results: There were 3228 subjects included in this Meta-analysis. The sensitivity and specificity [95% confidence interval (CI)] of CEA were 45.9% (43.2%-48.5%) and 97.0% (96.0%-97.8%), and of CYFRA 21-1, 47.3% (44.0%-50.6%) and 91.8% (89.5%-93.7%). AUC of CEA and CYFRA 21-1 were 0.7691 and 0.8213 respectively (Z=0.45, P>0.05). Conclusion: Both CEA and CYFRA 21-1 are useful for the differentiation of benign and malignant pleural effusion. (authors)

  11. 血清 CEA、CYFAR21-1、SCC、CA125水平对晚期非小细胞肺癌放疗疗效的评估价值%Assessment Value of Serum CEA,CYFAR21-1,SCC,CA125 Levels in Efficacy of Radiotherapy for Advanced Non-small Cell Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    阿合力·那斯肉拉; 巴尔夏古丽·扎比胡拉; 穆朝东

    2014-01-01

    serum CEA , CY-FAR21-1,SCC,CAl25 level before and after radiotherapy were detected by electrochemiluminescence .Results Effective rate of 176 cases of patients with radiotherapy was 58.0%,serum CEA,CYFAR21-1,SCC,CA125 levels in the observation group before radiotherapy were(33.65 ±5.41) ng/ml,(19.44 ±3.14) ng/ml,(16.87 ±2.74) ng/ml,(46.81 ±5.49) U/ml,the marker levels after radiotherapy were(25.13 ±4.65) ng/ml,(12.35 ±4.18) ng/ml,(11.77 ±3.04) ng/ml,(39.67 ±4.63) U/ml, which were significantly lower than those before radiotherapy ,the differences were statistically significant (P<0.05);serum tumor markers CEA,CYFAR21-1,SCC,CA125 levels in the control group before radiotherapy were (46.87 ±5.93) ng/ml,(28.63 ± 10.82) ng/ml,(26.46 ±8.68) ng/ml,(68.16 ±7.38) U/ml,the markers levels after radiotherapy were (52.17 ±5.49) ng/ml,(46.72 ±11.75) ng/ml,(38.74 ±63.48) ng/ml,(87.34 ±8.16) U/ml,which were significantly higher than radiotherapy , the differences were statistically significant (P<0.05);the markers levels before radiotherapy in the observation group were lower than those of the control group,the difference was significant(P<0.05);short-term efficacy of patients in the control group with decreased markers levels was better than those with elevated marker levels after radiotherapy ,the difference was statistical signifi-cant(χ2 =76.45,P<0.05).Conclusion Serum tumor markers CEA,SCC,CYFAR21-1,CA125 levels has certain evaluation value in curative effect of advanced non-small cell lung cancer treated with radiotherapy .

  12. In silico design, cloning and high level expression of L7/L12-TOmp31 fusion protein of Brucella antigens

    OpenAIRE

    Golshani, Maryam; Rafati, Sima; Jahanian-Najafabadi, Ali; Nejati-Moheimani, Mehdi; Siadat, Seyed Davar; Shahcheraghi, Fereshteh; Bouzari, Saeid

    2015-01-01

    Globally, Brucella melitensis and B. abortus are the most common cause of human brucellosis. The outer membrane protein 31 (Omp31) and L7/L12 are immunodominant and protective antigens conserved in human Brucella pathogens which are considered as potential vaccine candidates. We aimed to design the fusion protein from Brucella L7/L12 and truncated Omp31proteins, in silico, clone the fusion in pET28a vector, and express it in Escherichia coli host. Two possible fusion forms, L7/L12-TOmp31 and ...

  13. Balance 2003 of the risks control at the Cea

    International Nuclear Information System (INIS)

    As a research center on the energy, the information and health technologies and the defense, the Cea activities are indissociable from the risk control notion. To organize the risks management, the Cea decided to create in july 2003 a special pole of risks control and management. This presentation is based on some major topics of the risks control: the environmental impact control, the occupational risks control, the installations safety control and the hazardous matter transport control. (A.L.B.)

  14. Evaluation 2000 and regulation and method. Release monitoring and environmental surveillance around Cea centers

    International Nuclear Information System (INIS)

    This publication counts for the year 2000 for the evaluation of liquid and gaseous radioactive effluents releases and the radioactivity levels measured in the vicinity of Cea centers, through the air, water, vegetation and milk surveillance. An analysis of the results from 1996 to 2000 allows to follow their evolution. A second booklet develops the sampling and measurement methods made on effluents in environment. It present besides the regulation applied to effluents monitoring. (N.C.)

  15. Measurement of Circulating Filarial Antigen Levels in Human Blood with a Point-of-Care Test Strip and a Portable Spectrodensitometer.

    Science.gov (United States)

    Chesnais, Cédric B; Vlaminck, Johnny; Kunyu-Shako, Billy; Pion, Sébastien D; Awaca-Uvon, Naomi-Pitchouna; Weil, Gary J; Mumba, Dieudonné; Boussinesq, Michel

    2016-06-01

    The Alere Filariasis Test Strip (FTS) is a qualitative, point-of-care diagnostic tool that detects Wuchereria bancrofti circulating filarial antigen (CFA) in human blood, serum, or plasma. The Global Program to Eliminate Lymphatic Filariasis employs the FTS for mapping filariasis-endemic areas and assessing the success of elimination efforts. The objective of this study was to explore the relationship between the intensity of positive test lines obtained by FTS with CFA levels as determined by enzyme-linked immunosorbent assay (ELISA) with blood and plasma samples from 188 individuals who live in a filariasis-endemic area. The intensity of the FTS test line was assessed visually to provide a semiquantitative score (visual Filariasis Test Strip [vFTS]), and line intensity was measured with a portable spectrodensitometer (quantitative Filariasis Test Strip [qFTS]). These results were compared with antigen levels measured by ELISA in plasma from the same subjects. qFTS measurements were highly correlated with vFTS scores (ρ = 0.94; P bancrofti CFA levels in human blood, which are correlated with adult worm burdens. This tool may be useful for assessing the impact of treatment on adult filarial worms in individuals and communities. PMID:27114288

  16. HIV-1 Adaptation to Antigen Processing Results in Population-Level Immune Evasion and Affects Subtype Diversification

    DEFF Research Database (Denmark)

    Tenzer, Stefan; Crawford, Hayley; Pymm, Phillip;

    2014-01-01

    subtype-specific motifs. Multiple HLA variants presenting epitopes situated next to a given subtype-specific motif drive selection at this subtype-specific position, and epitope abundances correlate inversely with the HLA frequency distribution in affected populations. This adaptation reflects the sum of......The recent HIV-1 vaccine failures highlight the need to better understand virus-host interactions. One key question is why CD8(+) T cell responses to two HIV-Gag regions are uniquely associated with delayed disease progression only in patients expressing a few rare HLA class I variants when these...... regions encode epitopes presented by ~30 more common HLA variants. By combining epitope processing and computational analyses of the two HIV subtypes responsible for ~60% of worldwide infections, we identified a hitherto unrecognized adaptation to the antigen-processing machinery through substitutions at...

  17. Human serum levels of fetal antigen 1 (FA1/Dlk1) increase with obesity, are negatively associated with insulin sensitivity and modulate inflammation in vitro

    DEFF Research Database (Denmark)

    Chacón, M R; Miranda, M; Jensen, C H;

    2008-01-01

    Insulin Resistance), cytokines (sIL-6), adipokines (adiponectin) and circulating soluble fractions of tumor necrosis factor-alpha receptors 1 and 2 (sTNFR1 and sTNFR2). RESULTS: IN the obesity study, levels of FA1 in serum were found to increase with obesity. The S(i) index was negatively dependent on FA1......OBJECTIVE:To investigate fetal antigen 1 (FA1) protein within the context of human obesity and its relation with insulin sensitivity. SUBJECTS: Cross-sectional study that analyses circulating levels of FA1 in two selected human cohorts: n=127 men for the study of FA1 circulating levels in the...... context of obesity and insulin sensitivity (S(i)); and n=61 severely obese women before and after bariatric surgery. The response in vitro to FA1 protein on human cell lines of monocytes, preadipocytes and mature adipocytes was studied. MEASUREMENTS: Anthropometrical parameters: body mass index, waist...

  18. R and D developments on the disposal of radioactive wastes recently carried out at CEA (France)

    International Nuclear Information System (INIS)

    Since the law of december 31, 1991 voted by the French Parliament, disposal in geological formations is not the sole option considered for the management of radioactive wastes. Scientists and engineers, notably within the 'Commissariat a l'Energie Atomique' (CEA), must investigate in parallel two other options, namely i. advanced separation of long-lived radioisotopes and transmutation, and ii. improvement of radioactive waste matrices and long-term storage. Still, the geological disposal of radioactive wastes, which has generated within the last decade an immense amount of Research and Development, remains a major proposed solution to the long-term management of transuranic and high-level wastes. In this paper, we will describe some of the Research and Development carried out within CEA, avoiding the industrial and engineering aspects of the issues dealt with. Rather, we will illustrate major points of our basic research, underlining in particular methodological considerations

  19. Induction of Anti-Tumor Immunity Ex Vivo Using Dendritic Cells Transduced with Fowl Pox Vector Expressing MUC1, CEA, and a Triad of Costimulatory Molecules (rF-PANVAC)1

    OpenAIRE

    Vasir, Baldev; Zarwan, Corrine; Ahmad, Rehan; Crawford, Keith D; Rajabi, Hassan; Matsuoka, Ken-ichi; Rosenblatt, Jacalyn; Wu, Zekui; Mills, Heidi; Kufe, Donald; Avigan, David

    2012-01-01

    The fowl pox vector expressing the tumor associated antigens MUC1 and CEA in the context of costimulatory molecules (rF-PANVAC) has shown promise as a tumor vaccine. However, vaccine mediated expansion of suppressor T cell populations may blunt clinical efficacy. We characterized the cellular immune response induced by ex-vivo dendritic cells (DCs) transduced with (rF)-PANVAC. Consistent with the functional characteristics of potent antigen presenting cells, rF-PANVAC-DCs demonstrated strong ...

  20. Cancer antigen 125 and prognosis

    DEFF Research Database (Denmark)

    Høgdall, Estrid Vilma Solyom

    2008-01-01

    PURPOSE OF REVIEW: This review addresses recently reported progress in cancer antigen 125 as a prognostic marker in patients with ovarian cancer. RECENT FINDINGS: Serum cancer antigen 125 levels measured preoperatively in both early and late stage ovarian cancer may be of prognostic value. Before...... cancer antigen 125 determination may be implemented into clinical practice, cut-off levels must be evaluated and internationally defined. Studies examining serum cancer antigen 125 levels after surgery but before, during, or after treatment confirmed that changes in serum levels are of prognostic value....... Furthermore, recent studies have shown that the level of expression of cancer antigen 125 in tissue may be an independent prognostic indicator in late stage ovarian cancer. SUMMARY: Prognostic markers may potentially help to individualize treatment within subgroups of patients. In a recent study the level of...

  1. Progress in fusion reactors blanket analysis and evaluation at CEA

    International Nuclear Information System (INIS)

    In the frame of the recent CEA studies aiming at the development, evaluation and comparison of solid breeder blanket concepts in view of their adaptation to NET, the evaluation of specific questions related to the first wall design, the present paper examines first the performances of a helium cooled toroidal blanket design for NET, based on innovative Beryllium/Ceramics breeder rod elements. Neutronic and thermo-mechanical optimisation converges on a concept featured by a breeding capability in excess of 1.2, a reasonnable pumping power of 1% and a narrow breeder temperature range (470+-30 deg C of the breeder), the latter being largely independent of the power level. This design proves naturally adapted to ceramic breeder assigned to very strict working conditions, and provides for any change in the thermal and heat transfer characteristics over the blanket lifetime. The final section of the paper is devoted to the evaluation of the heat load poloidal distribution and to the irradiation effects on first wall structural materials

  2. Distinct patterns of blood-stage parasite antigens detected by plasma IgG subclasses from individuals with different level of exposure to Plasmodium falciparum infections

    DEFF Research Database (Denmark)

    Olesen, Cathrine Holm; Brahimi, Karima; Vandahl, Brian;

    2010-01-01

    -PAGE as high molecular weight ([greater than or equal to] 70 kDa) or low molecular weight (< 70 kDa). The number of discernable low molecular weight parasite antigens detected by different IgG subclass antibodies from each plasma sample was recorded. Using Wilcoxons rank sum test these reactivities were...... compared amongst groups of individuals with different levels of exposure to P. falciparum infections. RESULTS: IgG4 and IgM antibodies in plasma samples from all groups detected very few parasite antigens. IgG2 antibodies from all groups detected a common pattern of high molecular weight parasite antigens....... Cytophilic IgG subclasses in plasma samples from individuals with higher levels of exposure to P. falciparum infections distinctly detected higher numbers of low molecular weight parasite antigens. CONCLUSIONS: In the present study, there was no evidence for switching of antibody responses from non...

  3. Clinical indicators of envenoming and serum levels of venom antigens in patients bitten by Bothrops lanceolatus in Martinique. Research Group on Snake Bites in Martinique.

    Science.gov (United States)

    Bucher, B; Canonge, D; Thomas, L; Tyburn, B; Robbe-Vincent, A; Choumet, V; Bon, C; Ketterlé, J; Lang, J

    1997-01-01

    An enzyme-linked immunosorbent assay was developed to measure venom antigen levels in the serum of 40 patients bitten by Bothrops lanceolatus. The grading system used for the severity of envenomation (grades 1 to 4, minor to major) was predominantly based on the presence of local signs. Serum venom levels increased with the grade of severity (P or = 15 ng/mL were observed in all patients with progressive aggravation of swelling despite the use of early antivenom therapy. No venom was detectable in blood samples taken after completion of serotherapy. All patients recovered. These results confirm the efficacy of both the clinical severity scoring system used and the therapeutic regimen. PMID:9196765

  4. Fusion technology. Annual report of the. Association Cea/EURATOM

    International Nuclear Information System (INIS)

    In 1996, the French EURATOM-CEA Association made significant contributions to the European technology programme. This work is compiled in this report as follows: the ITER CEA activities and related developments are described in the first section; blankets and material developments for DEMO, long term safety studies are summarised in the second part; the Underlying Technology activities are compiled in the third part of this report. In each section, the tasks are sorted out to respect the European presentation. For an easy reading, appendix 4 gives the list of tasks in alphabetical order with a page reference list. The CEA is in charge of the French Technology programme. Three specific organizational directions of the CEA, located on four sites (see appendix 5) are involves in this programme: Advanced Technologies Direction (DTA), for Material task; Nuclear Reactors Direction (DRN), for Blanket design, Neutronic problems, Safety tasks; Physical Sciences Direction (DSM) uses the competence of the Tore Supra team in the Magnet design and plasma Facing Component field. The CEA programme is completed by collaborations with Technicatome, COMEX-Nucleaire and Ecole Polytechnique. The breakdown of the programme by Directions is presented in figure 1. The allocation of tasks is given in appendix 2 and in appendix 3, the related publications. (author)

  5. A Case of Radiation Fibrosis Appearing as Mass-Like Consolidation after SBRT with Elevation of Serum CEA

    Directory of Open Access Journals (Sweden)

    Kotaro Terashima

    2010-01-01

    Full Text Available We report a case of radiation fibrosis appearing as mass-like consolidation, which was difficult to distinguish from local recurrence. A 72-year-old woman was diagnosed as having primary lung cancer (cT1N0M0 stage IA in the right upper lobe and was treated with SBRT of 48 Gy in 4 fractions. After 12 months, mass-like consolidation appeared around the irradiated area, and after 13 months, it had increased in size. FDG-PET revealed high uptake (SUV max=5.61 for the consolidation. CT-guided biopsy was performed, but we could not confirm the diagnosis. Considering her poor respiratory function and her age, short-interval follow-up was performed. After 15 months, the consolidation enlarged at the dorsal side, and carcinoembryonic antigen (CEA became elevated (14.6 ng/mL. Serum KL-6 (436 U/mL and SP-D (204 ng/mL were also elevated. However, after 16 months, serum CEA slightly decreased. The consolidation gradually retracted on follow-up CT images. CEA, KL-6, and SP-D were also decreased by degrees. After 40 months, there is no evidence of local recurrence.

  6. Multimodality treatment of primary nonresectable intrahepatic cholangiocarcinoma with I-131 anti-CEA: A radiation therapy oncology group study

    International Nuclear Information System (INIS)

    Thirty-seven patients (57% with metastasis and/or who has previously undergone chemotherapy) with primary unresectable intrahepatic cholangiocarcinoma were prospectively treated with external beam radiation therapy, chemotherapy, and I-131 anti-carcinoembryonic antigen (CEA) antibody. The regimen led to partial remission in 26.6% of patients, according to CT scan digitized tumor volume analysis, and in 33.3% (25.9% with partial remission, 7.4% with complete emission) according to physical examination findings. Therapy began with whole liver irradiation (2.1 Gy, 0.3 Gy per fraction, delivered 4 days a week, with 10-MV photons) and, on alternate days, chemotherapy (Adriamycin, 15 mg, + 5-fluorouracil [5-FU], 500 mg). One month later, Adriamycin, 15 mg, + 5-FU, 500 mg, was administered on day 0; 20 mCi of I-131 anti-CEA on day 1; and 10 mCi of I-131 anti-CEA on day 6. Tumor effective half-life was 3-5 days. Median tumor dose (20 mCi + 10 mCi) was 6.2 Gy. Antibody therapy was administered in 2-month cycles. Grade IV thrombocytopenia and leukopenia each occurred in 3.2% of patient administrations. Median survival for the entire group was 6.5 months; for responders, it was 15.2 months. The longest partial remission is presently more than 4 years

  7. TIMP-1 and CEA as biomarkers in third-line treatment with irinotecan and cetuximab for metastatic colorectal cancer

    DEFF Research Database (Denmark)

    Spindler, Karen-Lise Garm; Christensen, Ib Jarle; Nielsen, Hans Jørgen; Jakobsen, Anders; Brünner, Nils

    2015-01-01

    colorectal cancer (CRC). The aim of the present study was to investigate the clinical value of TIMP-1 in patients with metastatic colorectal cancer (mCRC) treated with cetuximab and irinotecan. Patients with chemotherapy-resistant mCRC referred to third-line treatment with cetuximab (initial 400 mg/m(2......) followed by weekly 250 mg/m(2))/irinotecan (350 mg/m(2) q3w) were prospectively included in the biomarker study, as previously published. Pre-treatment blood samples were collected, and plasma TIMP-1 was measured by a validated in-house ELISA assay. In addition, carcinoembryonic antigen (CEA) measurement...... tumour localisation) or to differences in PFS or OS. The rank correlation between CEA and TIMP-1 was r = 0.50, and a test for interaction between TIMP-1 and CEA (dichotomised at 5 ng/ml) in survival analysis was not significant (p = 0.18). A multivariate analysis for PFS and OS resulted in a model with...

  8. 血清p53抗体联合CEA、CYFRA21-1检测在肺癌诊断中的价值%Role of combined detection of serum anti-p53 antibody with CEA and CYFRA21-1 in the diagnosis of lung cancer

    Institute of Scientific and Technical Information of China (English)

    杜紫燕; 余勇; 张增利; 李凌云; 邢玉斐; 施敏骅

    2013-01-01

    目的 探讨联合检测肺癌患者血清p53抗体与癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)辅助肺癌诊断的可行性.方法 在57例肺癌(肺癌组)和40例肺部良性疾病患者(对照组),采用ELISA法检测血清p53抗体,采用电化学发光免疫分析法检测血清CEA和CYFRA21-1.结果 肺癌组血清p53抗体、CEA和CYFRA21-1水平均高于对照组(P<0.05).p53抗体、CEA、CYFRA21-1曲线下面积(AUC)分别为0.770、0.814、0.779.p53抗体、CEA和CYFRA21-1的诊断界点分别为1.150 U/ml、3.095 ng/ml、3.240 ng/ml,敏感性分别为45.61%、66.67%、68.42%,特异性分别为87.50%、87.50%、85.00%.三项联合检测的AUC为0.902,敏感性为89.47%,特异性为100%.CEA与CYFAR21-1呈正相关(r=0.555,P<0.05).结论 血清p53抗体为独立的肺癌生物标记物,与CEA、CYFRA21-1联合检测有助于提高肺癌诊断水平.%Objective To explore the value of combined detection of serum anti-p53 antibody with carcinoembryonic antigen(CEA) and CYFRA21-1 in the diagnosis of lung cancer. Methods In 57 patients with lung cancer (group A) and 40 patients with benign pulmonary diseases (group B), serum levels of anti-p53 antibody, CEA and CYFRA 21-1 were measured by ELISA and electro-chemiluminescence immunoassay. Results Serum levels of anti-p53 antibody, CEA and CYFRA21-1 were higher in group A than those in group B(P<0. 05). The values of receiver operating characteristic area under the curve(AUC) for anti-p53 antibody,CEA and CYFRA 21-1 were 0. 770, 0. 814 and 0. 779, respectively. The cutoff levels of anti-p53 antibody, CEA and CYFRA 21-1 were 1. 150 U/ml,3. 095 ng/ml and 3. 240 ng/ml, respectively. The sensitivities of those markers for lung cancer detection were 45. 61%,66. 67%, 68. 42%, while the specificities were 87. 50%, 87. 50% and 85. 00% respectively. The value of AUC, sensitivity and specificity were 0. 90, 89. 47% and 100%, respectively,when combined detection of the three markers was

  9. Human leucocyte antigens in tympanosclerosis.

    Science.gov (United States)

    Dursun, G; Acar, A; Turgay, M; Calgüner, M

    1997-02-01

    This study was designed to evaluate the association between certain HLA antigens and tympanosclerosis. The serum concentrations of HLA antigens were measured by a microlymphocytotoxicity technique in patients with tympanosclerosis and compared with a healthy control group. The serum levels of HLA-B35 and -DR3 were significantly higher in the patients with tympanosclerosis. This result suggests that certain types of HLA antigens may play an important role as an indicator or mediator in the pathogenesis of tympanosclerosis. PMID:9088683

  10. Expressing Redundancy among Linear-Epitope Sequence Data Based on Residue-Level Physicochemical Similarity in the Context of Antigenic Cross-Reaction

    Science.gov (United States)

    2016-01-01

    Epitope-based design of vaccines, immunotherapeutics, and immunodiagnostics is complicated by structural changes that radically alter immunological outcomes. This is obscured by expressing redundancy among linear-epitope data as fractional sequence-alignment identity, which fails to account for potentially drastic loss of binding affinity due to single-residue substitutions even where these might be considered conservative in the context of classical sequence analysis. From the perspective of immune function based on molecular recognition of epitopes, functional redundancy of epitope data (FRED) thus may be defined in a biologically more meaningful way based on residue-level physicochemical similarity in the context of antigenic cross-reaction, with functional similarity between epitopes expressed as the Shannon information entropy for differential epitope binding. Such similarity may be estimated in terms of structural differences between an immunogen epitope and an antigen epitope with reference to an idealized binding site of high complementarity to the immunogen epitope, by analogy between protein folding and ligand-receptor binding; but this underestimates potential for cross-reactivity, suggesting that epitope-binding site complementarity is typically suboptimal as regards immunologic specificity. The apparently suboptimal complementarity may reflect a tradeoff to attain optimal immune function that favors generation of immune-system components each having potential for cross-reactivity with a variety of epitopes.

  11. Selection of aptamers to Anti-CEA%癌胚抗原特异性单克隆抗体(Anti-CEA)核酸适配体的筛选

    Institute of Scientific and Technical Information of China (English)

    曾家豫; 万波; 马瑾; 袁红霞; 李星; 赵运旺; 廖世奇

    2015-01-01

    目的:筛选癌胚抗原(carcinoembryonic antigen,CEA)特异性单克隆抗体(Anti-CEA)的核酸适配体(aptamers),为肺癌血清肿瘤标志物核酸适配体的筛选奠定实验基础.方法:利用羧基化琼脂磁珠作为筛选介质,以Anti-CEA为筛选的目标靶分子,通过消减SELEX技术及实时定量PCR技术,从随机ssDNA文库中筛选出与Anti-CEA特异性结合的aptamers,并通过凝胶阻滞实验(EMSA)鉴定筛选到的Anti-CEA-aptamer复合物,然后将得到的第10轮富集文库扩增为双链DNA,通过切胶纯化后,连接PMD18-T载体,转化大肠杆菌DH5α感受态细胞,同时利用交错PCR技术鉴定阳性克隆,经测序,获得aptamers的序列.结果:经过10轮筛选得到了4条与Anti-CEA结合的aptamers,测序结果显示均为不同序列.结论:验证筛选出的与Anti-CEA结合的aptamer,特异性检测结果表明2号aptamer与靶分子结合的特异性很高,且与非特异蛋白无明显吸附,筛选出的aptamers用于识别Anti-CEA,将为肺癌的早期诊断和早期治疗提供新的突破口.

  12. Contribution of genetic variation rs266882 to prostate-specific antigen levels in healthy controls with serum PSA below 2.0 ng/ml.

    Science.gov (United States)

    Song, Jaeman; Park, Heeyoon; Lee, Gilho

    2013-04-01

    We evaluated the impact of genetic variation in the prostate-specific antigen (PSA) gene (rs266882) on serum PSA levels in healthy men as well as risk factors for benign prostate hypertrophy (BPH) and prostate cancer. The study population comprised 91 men with PSA levels below 2.0 ng/ml as healthy controls, 78 men with PSA 2-10 ng/ml as a BPH group, and 128 prostate cancer patients, all in Korea. DNA was amplified by polymerase chain reaction and the product was sequenced. We found that PSA levels were associated with a G/A polymorphism only in healthy controls. The transition, however, was not associated with PSA levels of BPH and cancer patients, nor was it a risk factor. In conclusion, this genetic factor is important for determining serum PSA levels in the naive group, whereas the disruption of prostatic architecture in BPH or prostate cancer may be a major determining factor for PSA levels. PMID:23315126

  13. 2nd CEAS Specialist Conference on Guidance, Navigation and Control

    CERN Document Server

    Mulder, Bob; Choukroun, Daniel; Kampen, Erik-Jan; Visser, Coen; Looye, Gertjan

    2013-01-01

    Following the successful 1st CEAS (Council of European Aerospace Societies) Specialist Conference on Guidance, Navigation and Control (CEAS EuroGNC) held in Munich, Germany in 2011, Delft University of Technology happily accepted the invitation of organizing the 2nd  CEAS EuroGNC in Delft, The Netherlands in 2013. The goal of the conference is to promote new advances in aerospace GNC theory and technologies for enhancing safety, survivability, efficiency, performance, autonomy and intelligence of aerospace systems using on-board sensing, computing and systems. A great push for new developments in GNC are the ever higher safety and sustainability requirements in aviation. Impressive progress was made in new research fields such as sensor and actuator fault detection and diagnosis, reconfigurable and fault tolerant flight control, online safe flight envelop prediction and protection, online global aerodynamic model identification, online global optimization and flight upset recovery. All of these challenges de...

  14. Anti-CEA aptamers labeled with 99mTc: encapsulation studies in long-circulating and pH-sensitive liposomes, biodistribution and imaging

    International Nuclear Information System (INIS)

    Colorectal cancer (CRC) is one of the leading cancers and the carcinoembryonic antigen (CEA) is a tumor marker widely used in diagnosis since it is overexpressed in tumor cells. Acid nucleic aptamers with high affinity and specificity for this antigen become promising molecules for CRC diagnosis by imaging. However, due to the action of nucleases in vivo, they have been investigated for association with liposomes, such as long-circulating and pH-sensitive liposomes (SPHL) that can be destabilized in the tumor region releasing aptamers and contributing to the CRC diagnosis by scintigraphy. In this work, SpHL containing DOPE, CHEMS and mPEG2000-DSPE were characterized by analyzing mean diameter, polydispersity index and zeta potential. The anti-CEA aptamers Apt3 and Apt3-Amino were labeled with technetium-99m and the encapsulation efficiency (EE) of 99mTc-Apt3 in the SpHL by dehydration-rehydration (modified DRV) and freeze-thaw (FT) were analyzed, both in the presence of cryoprotectants. Biodistribution and scintigraphic images were performed at 1h and 4h post-injection of 99mTc-Apt3-amino, 99mTc-Apt3-SpHL or 99mTc-Apt3-amino-SpHL complexes in Balb/c healthy mice. The SpHL dispersions were homogeneous. The radiolabeling yield with technetium-99m was over 90% for all complexes. By the dehydration-rehydration method, the SpHL increased over 200% after encapsulation procedure. By the freeze-thaw method, the SpHL size increased only 13.7%. Free 99mTc-Apt3-amino showed to be cleared by renal via with high levels of radioactivity in the kidney and bladder, however, the 99mTc-Apt3-SpHL and 99mTc-Apt3-amino-SpHL clearly indicated high uptake by liver and spleen. The biodistribution of 99mTc-Apt3-SpHL showed significant uptake of radioactivity by stomach and thyroid indicating less stability of the Apt3 radiolabelling in relation to Apt3-amino. (author)

  15. Anti-CEA aptamers labeled with {sup 99m}Tc: encapsulation studies in long-circulating and pH-sensitive liposomes, biodistribution and imaging

    Energy Technology Data Exchange (ETDEWEB)

    Leonel, M.F.V.; Andrade, A.S.R. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Oliveira, M.C.; Cardoso, V.N.; Barros, A.L.B. [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Faculdade de Farmacia

    2015-07-01

    Colorectal cancer (CRC) is one of the leading cancers and the carcinoembryonic antigen (CEA) is a tumor marker widely used in diagnosis since it is overexpressed in tumor cells. Acid nucleic aptamers with high affinity and specificity for this antigen become promising molecules for CRC diagnosis by imaging. However, due to the action of nucleases in vivo, they have been investigated for association with liposomes, such as long-circulating and pH-sensitive liposomes (SPHL) that can be destabilized in the tumor region releasing aptamers and contributing to the CRC diagnosis by scintigraphy. In this work, SpHL containing DOPE, CHEMS and mPEG{sub 2000}-DSPE were characterized by analyzing mean diameter, polydispersity index and zeta potential. The anti-CEA aptamers Apt3 and Apt3-Amino were labeled with technetium-99m and the encapsulation efficiency (EE) of {sup 99m}Tc-Apt3 in the SpHL by dehydration-rehydration (modified DRV) and freeze-thaw (FT) were analyzed, both in the presence of cryoprotectants. Biodistribution and scintigraphic images were performed at 1h and 4h post-injection of {sup 99m}Tc-Apt3-amino, {sup 99m}Tc-Apt3-SpHL or {sup 99m}Tc-Apt3-amino-SpHL complexes in Balb/c healthy mice. The SpHL dispersions were homogeneous. The radiolabeling yield with technetium-99m was over 90% for all complexes. By the dehydration-rehydration method, the SpHL increased over 200% after encapsulation procedure. By the freeze-thaw method, the SpHL size increased only 13.7%. Free {sup 99m}Tc-Apt3-amino showed to be cleared by renal via with high levels of radioactivity in the kidney and bladder, however, the {sup 99m}Tc-Apt3-SpHL and {sup 99m}Tc-Apt3-amino-SpHL clearly indicated high uptake by liver and spleen. The biodistribution of {sup 99m}Tc-Apt3-SpHL showed significant uptake of radioactivity by stomach and thyroid indicating less stability of the Apt3 radiolabelling in relation to Apt3-amino. (author)

  16. CEA's Implication in Nuclear Security Events Detection and Response

    International Nuclear Information System (INIS)

    In France, CEA1 is a key player in research, development and technical innovation in three main areas: energy, heath and information technologies, defence and global security. For example CEA has developed a multitude of technologies to fight nuclear, radiological, biological and chemical threats, as well as equipment to diagnose and assess radiological or nuclear improvised explosive devices. CEA is also involved in all technical aspects dealing with detection of and response to a nuclear security event. CEA is a major player in the operational response to a terrorist or criminal act involving Nuclear or Radiological material. He operates under DCI-IT2, a dedicated national structure which includes the necessary personnel to neutralize the threat. CEA is also in charge of advice and assist competent authorities in the management of a nuclear security event. The paper clarify CEA’s implication in detection of and response to a nuclear security event and position in the French organization for operational response, elements of the intervention strategy - research to neutralize the threat - as well as examples of technical achievements that most are made subject to transfer to an industrial. In the context of the common declaration from France-United Kingdom-United States of America governments at Seoul summit in March 2012 to engage to further strengthen worldwide preparedness to contend with the threat of nuclear terrorism, France has capability to response to a nuclear threat device. This document will support this declaration and will give examples of CEA involvement in Nuclear Security system and measures. (author)

  17. CEA 2011, a look back at a year of research

    International Nuclear Information System (INIS)

    For this year 2011, marked by the Fukushima accident, the goal is improved safety. This is a key issue being addressed by CEA, while at the same time boosting its R and D on low-carbon energies, defense and security, Health technologies, information technologies and very large research Infrastructures. With more than 650 priority patents filed in 2011, CEA maintains its position as the leading French research organization. CEA stands in 4. place among the European research organizations, in terms of the number of projects (535, including 70 that it coordinates) and financing obtained (nearly euros 55 M) under the European Commission's FP7 framework programme. CEA's civil programs are 30% funded from external revenues (partner companies, national incentive funds, local authorities and European Union), 49% from the Government and, finally, 21% from two funds dedicated to clean-up of civil and defense facilities. Of the euros 1, 391 M devoted to low-carbon energies, research into the new energy technologies and nuclear systems of the future each received a budget of euros 151 M in 2011. CEA plays a key role in the European Energy Research Alliance, as a founding member, a member of the executive committee and a member of the secretariat. It is France's representative. EERA has launched 13 joint programs, including 6 in 2011. It pools the research efforts of more than 150 institutes and universities, with more than 2, 000 staff employed full-time. CEA is present in 8 programs, with 100 full-time staff, in other words 5% of the total partner commitment. This special issue of 'Defis du CEA' journal deals with the main results of the researches carried out in 2011 at the CEA. Contents: 1 - Facts and figures 2011: Intellectual Property, European Financing, Budget, Nuclear Safety, European Research Programs, International Relations, International Collaboration, Spin-off, Scientific Excellence, Training; 2 - Low-carbon energies: Biofuels, Solar Photovoltaic, Nuclear, Energy

  18. Report transparency and nuclear safety 2007 CEA Grenoble

    International Nuclear Information System (INIS)

    This report presents the activities of the CEA Center of Grenoble for the year 2007. Since 2002 the Passage project aims to realize the decontamination and the dismantling of old nuclear installations of the CEA Grenoble. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the year 2007 saw two main steps of the Passage project: the decommissioning of the Siloette reactor, a public consultation about the Lama laboratory dismantling. (A.L.B.)

  19. The CEA-industrie Group. Report for 1986

    International Nuclear Information System (INIS)

    From the end of 1983, the whole affiliates and participations of CEA are hept by CEA-industry. The group activity is principally in the nuclear field. Cogema and the whole fuel cycle, construction, maintenance, improvement of nuclear power plants, research reactors and naval propulsion boilers and the sector Power plants and nuclear services (Framatome, Technicatome, Intercontrole, Stmi, principally). A non-nuclear sector comes from new techniques or activities developed for the nuclear needs: computer science (CISI), biomedical field (ORIS-industrie), different activities such as ultrafiltration or robotics-productics

  20. Remote manipulators and mobile robots in the CEA

    International Nuclear Information System (INIS)

    Thanks to Jean Vertut's works in the seventies, the CEA has been a pioneer in nuclear remote manipulation and mobile robots conception and has developed innovating systems in response to industry request. During the nineties, improvements in computer control systems have led to the realization of functional demonstrators. In 1995, advances in technology have allowed to consider the first industrial applications of nuclear remote manipulators and robots. This paper gives a review of the development and research works carried out by the CEA in this domain since the seventies, and also some future prospects. (J.S.). 10 figs

  1. CEA Bolometer Arrays: the First Year in Space

    OpenAIRE

    Billot, Nicolas; Sauvage, M.; RODRIGUEZ, L.; Horeau, B.; Kiss, C.; Aussel, H.; Okumura, K; O. Boulade; Altieri, B.; Poglitsch, A.; Agnese, P.

    2010-01-01

    The CEA/LETI and CEA/SAp started the development of far-infrared filled bolometer arrays for space applications over a decade ago. The unique design of these detectors makes possible the assembling of large focal planes comprising thousands of bolometers running at 300 mK with very low power dissipation. Ten arrays of 16x16 pixels were thoroughly tested on the ground, and integrated in the Herschel/PACS instrument before launch in May 2009. These detectors have been successfully commissioned ...

  2. The opening of the CEA to the general public

    International Nuclear Information System (INIS)

    Full text: The relationships between science and society have evolved considerably over the past years, just like the perception of the nuclear industry in the French public opinion. Recent psychosociological surveys show that only half of the French population is familiar with the CEA and that the public would like to obtain new elements of judgment in order to be able to develop a direct appreciation of the activities of a research organization such as the Atomic Energy Commission. It is essential to meet the public's expectations, to keep it properly informed of the CEA's research activities, and to help it understand the relevance of the results obtained and the solutions offered to decision-makers, i.e. industrialists and public authorities. One way to answer the public opinion's questions and to meet its expectations is to allow a rowing number of visitors to see who we are, what we study in our laboratories, and how we work to contribute to scientific progress and to the diffusion of technology, in a manner that is useful to society as a whole. This describes the spirit and the objective that governed the very idea and elaboration of the program entitled 'Opening of the CEA to the general public'. This operation consists not only in having communication specialists guide visitors throughout the facilities and equipment of the CEA, but also in encouraging researchers and groups of visitors to meet in the laboratories. A 'CEA - communication' network is under construction. It already has nearly 600 members. Communication is considered to be a real calling that falls within the scope of the CEA's strategy, and the work accomplished in this respect is recognized to the same extent as research. To this end, a charter for the CEA - communication network will be signed by every contributor and manager. A training scheme focused on public speaking and scientific vulgarization was initiated. 50 members of the network have already been trained, and 250 will be in 1999

  3. Cea strategy in the field of simulation and high-performance computing

    International Nuclear Information System (INIS)

    The full text of the publication follows. Modelling and Simulation are at the heart of research and development activities carried out by Cea. Modelling relies not only on the most advanced theories and on phenomenological experiments that ensured their tuning, but also on more instrumented experiments that deliver both the useful data for the implementation of these models and the elements required for their validation. Simulation not only covers an as close as possible representation of the physical reality, but more generally all numerical experimentation carried out with any model. In particular, there are scientific problems that cannot be solved by simulation alone. Cea makes use of simulation for understanding, for design, and for taking actions. Particle physics, climatology, the analysis and comparison of genomic sequences, the study of dynamics and folding of proteins, material physics or high temperature plasma physics illustrate the theme of simulation 'for understanding'. Designing fission or fusion reactors of the future, or nuclear weapons may use of simulation for design. Real-time simulators that aim at training operators of nuclear power plants, as well as simulations carried out to prove nuclear safety, belong to the third category. Technological evolution in the field of computers, software, or algorithms, or more generally progress made in the field of modelling have enabled Cea and others to advance significantly in regard to simulation. The hierarchization of models and multi-scale simulation techniques permit in fact the use of results stemming from milli-metric, micro-metric or even nano-metric level simulations for models that are representative of the system at the metric level. The same is true for time-scales. The rendering common place of workstations or PC-cluster based department servers, readily offering a performance of the order of a Giga-flop or tens of Giga-flops, makes it possible to meet the large majority of Cea needs. The

  4. Correlation of mRNA and protein levels: Cell type-specific gene expression of cluster designation antigens in the prostate

    Directory of Open Access Journals (Sweden)

    Deutsch Eric W

    2008-05-01

    Full Text Available Abstract Background: Expression levels of mRNA and protein by cell types exhibit a range of correlations for different genes. In this study, we compared levels of mRNA abundance for several cluster designation (CD genes determined by gene arrays using magnetic sorted and laser-capture microdissected human prostate cells with levels of expression of the respective CD proteins determined by immunohistochemical staining in the major cell types of the prostate – basal epithelial, luminal epithelial, stromal fibromuscular, and endothelial – and for prostate precursor/stem cells and prostate carcinoma cells. Immunohistochemical stains of prostate tissues from more than 50 patients were scored for informative CD antigen expression and compared with cell-type specific transcriptomes. Results: Concordance between gene and protein expression findings based on 'present' vs. 'absent' calls ranged from 46 to 68%. Correlation of expression levels was poor to moderate (Pearson correlations ranged from 0 to 0.63. Divergence between the two data types was most frequently seen for genes whose array signals exceeded background (> 50 but lacked immunoreactivity by immunostaining. This could be due to multiple factors, e.g. low levels of protein expression, technological sensitivities, sample processing, probe set definition or anatomical origin of tissue and actual biological differences between transcript and protein abundance. Conclusion: Agreement between these two very different methodologies has great implications for their respective use in both molecular studies and clinical trials employing molecular biomarkers.

  5. Unusual prostate carcinoma characterized by extensive metastasis, significantly increased serum level of prostatic-specific antigen, and neuroendocrine differentiation: a case report

    Institute of Scientific and Technical Information of China (English)

    HU Yuxin; YE Juan; JIANG Ying; ZHANG Qin-fang; WU Yue-long; CHEN Yue-yu

    2005-01-01

    @@ Some rare variants of prostate carcinoma have been described in recent years.1-3 In this article we report a man with uncommon prostate carcinoma with the following three pathological characteristics: (a) extensive metastasis to bone and lymph nodes of the abdomen, pelvis, and supraclavicular area; (b) significantly increased serum level of prostatic-specific antigen (PSA) as high as 1800 ng/ml; and (c) partial neuroendocrine differentiation in cancer tissue. The patient died 7 months after pathological diagnosis or 22 months after appearance of initial signs. This case has drawn our attention to the fact that pathological diversity of prostate neoplasm might easily lead to misdiagnosis or to delayed diagnosis, and moreover, reasonable therapy for such a case should be based on a thorough investigation. On the other hand, early initiation of appropriate treatment of advanced neuroendocrine carcinoma may improve the prognosis.

  6. Zinc oxide nanoparticle-enhanced ultrasensitive chemiluminescence immunoassay for the carcinoma embryonic antigen

    International Nuclear Information System (INIS)

    An ultrasensitive enzyme-linked immunosorbent assay (ELISA) is reported for the determination of carcinoma embryonic antigen (CEA) in human serum. It was realized using a microplate reader using a 384-well plate. Monoclonal antibody (Ab) against CEA (1° Ab) acting as the capture probe was immobilized on zinc oxide nanoparticles (ZnO-NPs) in the form of self-assembled monolayers (SAMs). CEA captured by 1° Ab was quantified using a sandwich ELISA wherein a polyclonal second antibody against CEA (2° Ab) was used for detection and quantified using an HRP-labeled secondary antibody (3° Ab). The ZnO-NPs-CEA capture probe was deposited on the bottom of the wells in order to enhance capture of CEA. A 3-fold enhancement in the chemiluminescence (CL) signal of luminol is found (compared to a conventional ELISA). CEA can be quantified by this method in concentrations as low as 1 pg · mL−1. The upper limit of detection is 20 ng · mL−1. The use of ZnO-NPs also imparts improved thermal stability. When stored at 4 °C in phosphate-buffered saline of pH 7.4, the probe displays stability of up to 30 days. (author)

  7. Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

    Directory of Open Access Journals (Sweden)

    Adnan Ahmad

    2011-06-01

    Full Text Available Abstract Background A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, the Hepatitis B surface antigen (HBsAg. Thus, the Hepatitis B vaccine is generated through the utilization of recombinant DNA technology, preferentially by using yeast-based expression systems. However, the polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs, to elicit the required protective immune response. So far, no clear evidence has been presented showing whether HBsAg assembles in vivo inside the yeast cell into VLPs or later in vitro during down-stream processing and purification. Results High level production of HBsAg was carried out with recombinant Pichia pastoris using the methanol inducible AOX1 expression system. The recombinant vaccine was isolated in form of VLPs after several down-stream steps from detergent-treated cell lysates. Search for the intracellular localization of the antigen using electron microscopic studies in combination with immunogold labeling revealed the presence of HBsAg in an extended endoplasmic reticulum where it was found to assemble into defined multi-layered, lamellar structures. The distance between two layers was determined as ~6 nm indicating that these lamellas represent monolayers of well-ordered HBsAg subunits. We did not find any evidence for the presence of VLPs within the endoplasmic reticulum or other parts of the yeast cell. Conclusions It is concluded that high level production and intrinsic slow HBsAg VLP assembly kinetics are leading to retention and accumulation of the antigen in the endoplasmic reticulum where it assembles at least partly into defined lamellar structures. Further transport of HBsAg to the Golgi apparatus is impaired thus leading to secretory pathway disfunction and the formation of an extended endoplasmic reticulum which bulges into irregular cloud-shaped formations. As VLPs were

  8. Development of solid phase immunoradiometric assay for determination of carcinoembryonic antigen as a tumor marker

    International Nuclear Information System (INIS)

    Development of solid phase coated tube immunoradiometric assay for estimation of carcinoembryonic antigen (CEA) was the aim of the present study. Labeling of CEA was carried out using Ch-T and iodogen as oxidizing agents and 125I. The tracers were used to test the presence of antibodies produced by immunization. Production of polyclonal antibody was carried out through immunization of four mice. After purification step, the tubes were coated by purified polyclonal antibodies. Immunoradiometric that system was performed using the commercial IZOTOP 125I-anti hCEA tracer then the validity studies were carried out. The results show that the local coated tubes made the assay is more than sufficient to fulfill the clinical requirement of CEA as a tumor marker. (author)

  9. Iridium Oxide Film-Enhanced Impedance Immunosensor for Rapid Detection of Carcinoembyronic Antigen

    Institute of Scientific and Technical Information of China (English)

    DING,Yan-Jun; WANG,Hua; JIANG,Jian-Hui; SHEN,Guo-Li; YU,Ru-Qin

    2007-01-01

    A simple, rapid and sensitive impedance immunosensor based on iridium oxide (IrOx) thin film for the detection of carcinoembyronic antigen (CEA) in human sera has been proposed. Gold electrode was electrochemically modified with IrOx thin film and simultaneously functionalized with protein A (PA) to bind anti-CEA antibodies in an orientated way. It has been found that the antibody loading amount was dependent on the PA concentration and the deposition time of IrOx matrix. Under the optimized experimental conditions, the electron transfer resistances obtained were linearly related to the CEA concentration ranging from 36.2 to 460.0 ng/mL, with a detection limit of 28.0 ng/mL. Analytical results of clinical samples from cancer patients show that the proposed immunoassay is reasonably comparable with the chemiluminescence immunoassay (CLIA), indicating the feasibility of using the proposed method for CEA immunoassay in clinical laboratory.

  10. Cell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains

    DEFF Research Database (Denmark)

    Dellgren, Christoffer; Nehlin, Jan O; Barington, Torben

    2015-01-01

    . Moreover, recent observations suggest that even minor differences in expression levels may influence the course of viral infections and the frequency of complications to stem cell transplantation. We have shown that some human multipotent stem cells have high expression of HLA-A while HLA-B is only weakly......Constitutive cell surface expression of Human Leukocyte Antigen (HLA) class I antigens vary extremely from tissue to tissue and individual antigens may differ widely in expression levels. Down-regulation of class I expression is a known immune evasive mechanism used by cancer cells and viruses...... expressed, and demonstrate here that this is also the case for the human embryonic kidney cell line HEK293T. Using quantitative flow cytometry and quantitative polymerase chain reaction we found expression levels of endogenous HLA-A3 (median 71,204 molecules per cell) 9.2-fold higher than the expression of...

  11. How Packaging Fleet Renewal Fits French CEA Programs

    International Nuclear Information System (INIS)

    CEA's (French Atomic Energy Agency) packaging fleet is dedicated to transportation of test irradiated fuels, of research reactors fuels, of navy propulsion fuels, and of waste coming from and to nuclear plants or facilities. This fleet encompasses more than 30 types of casks ranging from 5 to 30 tons, with either recent designs or other dating back to the seventies. A study has been launched in order to perform a global analysis of the life expectancy of the existing CEA and COGEMA Logistics cask fleets with respect to a 2015 target, in order to anticipate its renewal, while limiting the number of type of cask. Key elements like periodical evolutions of design and transport regulations, lessons learnt of existing casks (design, approval and extensions, operational feedback, maintenance and dismantling) are taken into account in order to ensure compliance and availability of the fleet. Moreover, from design to cask delivery, including regulatory tests, safety analysis report/ CoC, and manufacturing, 3 to 5 years is needed. Therefore cask development should be taken into account earlier of invest and research's programs. The paper will address the current life expectancy study of CEA and COGEMA Logistics packaging fleet, based on lessons learnt and regulation evolution and on general R and D plans by user facilities. It will show how a comprehensive optimized fleet is made available to CEA and other customers. Such a fleet combines optimized investment and uses, thus entailing synergies for well-mastered costs of transports. (authors)

  12. Behaviour of heavy water in nuclear reactors of the CEA

    International Nuclear Information System (INIS)

    In the two heavy water reactors of the CEA: Zoe and P-2, we do: A) the supervision of the isotopic composition of the heavy water; B) the supervision of gases released by the decomposition of the heavy water under radiation, and to their recombination; C) periodic analyses of impurities. (M.B.)

  13. Dose-response association between hepatitis B surface antigen levels and liver cancer risk in Chinese men and women.

    Science.gov (United States)

    Yang, Yang; Gao, Jing; Li, Hong-Lan; Zheng, Wei; Yang, Gong; Zhang, Wei; Ma, Xiao; Tan, Yu-Ting; Rothman, Nathaniel; Gao, Yu-Tang; Chow, Wong-Ho; Shu, Xiao-Ou; Xiang, Yong-Bing

    2016-07-15

    We aimed at evaluating the risk of liver cancer in different levels of HBsAg among Chinese men and women. We carried out a nested case-control study including 363 cases and 3,511 controls in two population-based cohorts in Shanghai. Plasma samples collected at enrollment were quantified for HBsAg levels using the Architect QT assay. Conditional logistic regression was performed to estimate the odds ratios (ORs) and 95% confidence intervals (95% CIs) for liver cancer, with adjustment for potential confounders. HBsAg was detected in 6.29% of control subjects overall (7.02% in men and 4.98% in women). HBsAg levels were positively associated with liver cancer risk in a dose-response manner (ptrend  < 0.001). Such association showed a significant gender disparity. With increasing levels of HBsAg, liver cancer risks rose more steeply in men than in women. In men, the adjusted ORs increased from 7.27 (95% CI: 3.49-15.15) at the lowest detectable level of HBsAg (5-9 IU/ml) to 7.16 (95% CI: 3.21-15.96), 34.30 (95% CI: 16.94-69.44), and 47.33 (95% CI: 23.50-95.34) at the highest level of HBsAg (≥1,000 IU/ml) compared to those negative for HBsAg. The corresponding ORs were much lower for women, from 1.37 (95% CI: 0.25-7.47), 3.81 (95% CI: 1.09-13.28), 7.36 (95% CI: 2.41-22.46) and 16.86 (95% CI: 7.24-39.27), respectively. HBsAg quantification has potential to distinguish individuals at different risks of liver cancer. Men with the lowest detectable level of HBsAg should still pay attention to their liver cancer risks, but those with a higher level may be given a higher priority in future liver cancer surveillance program. PMID:26990915

  14. CEA decommissioning strategy and program for nuclear facilities

    International Nuclear Information System (INIS)

    The CEA's experience of the decommissioning of nuclear facilities goes back many decades and relates to many installations of very different types. The first substantial operations began in the sixties and seventies, and included, for instance, the first plutonium plant at Fontenay-aux-Roses (total decommissioning) and a number of small research reactors and critical mock-ups such as Cesar and Peggy at Cadarache, and Minerve at Fontenay-aux-Roses. Some twenty facilities were dealt with by 2000, corresponding to around half of all the nuclear facilities permanently closed, beginning with the decommissioning of facility AT1 at La Hague, the pilot plant used by the CEA in the seventies for the reprocessing of spent fuel from fast neutron reactors in March 2001 and ending with the demolition of the G1 stack at Marcoule, on 19 July this year. During the nineties, decommissioning was scaled back and few of the operations in progress were completed. The end of the decade saw a slowdown which was a consequence of financial difficulties, as the CEA had not yet set aside sufficient funds to cover the work, even though, between 1993 and 1999, part of the necessary resources had been provided under an agreement with the industrial partners EDF and Cogema. At the present time, the situation is rapidly improving both as concerns organisational and financial issues for the CEA and as concerns the regulations and the management of waste for all the organisations involved. First of all, for the different problems associated with radioactive clean-up faced by its research centres, the efforts made by the CEA in the field of organisation and planning since the beginning of the nineties have led to the drawing up of an overall multi-year plan referred to as the 'CEA civil centre radioactive clean-up plan' and the appointment of the Legacy and Clean-up Directorate to act as the client and be in charge of application of the plan relating not only to decommissioning of the nuclear

  15. Clinical significance of combined detection of CYFRA21-1, NSE and CEA in classification and staging of patients with lung cancer

    International Nuclear Information System (INIS)

    To explore clinical value of combined detection of CYFRA21-1, NSE and CEA in classification and staging of patients with lung cancer, the CYFRA21-1, NSE and CEA levels in pleural effusion in 330 patients with lung cancer and in 43 patients with benign were detected by the electrochemiluminescence. The results showed that CYFRA21-1, NSE and CEA levels in pleural effusion in patients with lung cancer group were significantly higher than that of in benign group (P<0.01). The positive rate of tumor markers in different pathological type lung cancer were different,which CYFRA21-1 positive rate in squamous cell cancer group was highest with 65.5%; CEA positive rate in glands cancer group was supreme with 65.0%; the NSE positive rate in differentiation cancer group was highest with 79.5%. The positive rate in three markers combined detection was higher than that in one item detection. The tumor marker levels in lung cancer were positively related with clinical staging. The higher of tumor marker levels and the more late of clinical staging, and the clinical III∼IV period was obviously higher than that I∼II period (P<0.05). The combined detection of CYFRA21-1, NSE and CEA may enhance the positive rate in lung cancer detection, and may have significant clinical value in the classification and staging of patients with lung cancer. (authors)

  16. Cytokeratins and carcinoembryonic antigen in diagnosis, staging and prognosis of colorectal adenocarcinoma

    Institute of Scientific and Technical Information of China (English)

    Luís C. Fernandes; Su B. Kim; Delcio Matos

    2005-01-01

    AIM: To evaluate the serum levels of cytokeratins and carcinoembryonic antigen (CEA) in diagnosis, staging and prognosis of patients with colorectal adenocarcinoma.METHODS: The sample consisted of 169 patients. One hundred blood donors formed the control group. Radical surgery was performed on 120 patients, with an average follow-up duration of 22.3 mo. Relapses occurred in 23individuals after an average of 18.09 mo. CEA was assayed via the Delfia(R) method with a limit of 5 ng/mL. Cytokeratins were assayed via the LIA-mat(R) TPA-M Prolifigen(R) method with a limit of 72 U/L.RESULTS: In the diagnosis of patients with colorectal adenocarcinoma, CEA showed a sensitivity of 56%, a specificity of 95%, a positive predictive value of 94%, a negative predictive value of 50% and an accuracy of 76.8%.TPA-M had a sensitivity of 70%, a specificity of 96%, a positive predictive value of 97%, a negative predictive value of 66% and an accuracy of 93.6%. The elevation of one of the markers was shown to have a sensitivity of 76.9%, a specificity of 91%, a positive predictive value of 93.5%, a negative predictive value of 70% and an accuracy of 83.6%.There was no variation in the levels of the markers according to the degree of cell differentiation while there was an elevation in their concentrations in accordance with the increase in neoplastic dissemination. There was a statistically significant difference between the patients with stage Ⅳ lesions and those with stages Ⅰ, Ⅱ and Ⅲ tumors.With regard to CEA, the averagelevel was 14.2 ng/mL in patients with stage Ⅰ lesions, 8.5 ng/mL in patients with stage Ⅱ lesions, 8.0 ng/mL in patients with stage Ⅲ lesions and 87.7 ng/mL in patients with stage Ⅳ lesions. In relation to TPA-M, the levels were 153.1 U/L in patients with stage Ⅰtumors, 106.5 U/L in patients with stage Ⅱ tumors, 136.3 U/L in patients with stage Ⅲ tumors and 464.3 U/L in patients with stage Ⅳ tumors. There was a statistical difference in

  17. 晚期非小细胞肺癌患者血清癌胚抗原水平和全身转移能力的风险评估%Serum Level of Carcinoembryonic Antigen and Whole-body Metastasis Ability Risk Assessment in Patients with Advanced Non-small Cell Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    李捷; 李付广; 常保萍; 焦江琴

    2015-01-01

    Objective: To study the serum level of carcinoembryonic antigen and whole-body metastasis ability risk in patients with advanced non-small cell lung cancer,and provide the reference for clinical.Method: 160 patients with stage IV NSCLC in our hospital from March 2012 to January 2014 were selected. Research indicators included age, height,weight,age,sex,histological classification,tumor differentiation ,CEA level,smoking status,bone metastases,lymphatic metastasis,obstinate/transfer, transfer, soft tissue of neck metastases, brain metastases, systemic metastasis score and systemic metastasis LN-adjustment score, and the statistical analysis was performed.Result: The sex,bone metastases,histological classification lymph node metastasis and brain metastasis of different CEA level patients had statistical significance (P0.05).Serum CEA level increased from 2.7 ng/mL in LN-adjusted whole-body metastatic score 1 to 374.1 ng/mL in LN-adjusted whole-body metastatic score 7,the difference was statistically significant (P=0.003).Similarly, the low LN-adjusted whole-body metastatic score (rating 1-3) showed CEA level significantly reduced when compared with adjustment of high LN-adjusted whole-body metastatic score (rating 4-7),the difference was statistically significant(P0.05)。血清CEA水平从LN-调整全身转移得分1分中的2.7 ng/mL上升至LN-调整全身转移得分7分中的374.1 ng/mL(P=0.003)。低LN-调整全身转移得分(评分1~3分)与高LN-调整全身转移得分(评分4~7分)比较表现出CEA水平显著降低,两组比较差异具有统计学意义(P<0.001)。血清高CEA水平的比例从全身转移得分1分中占37.5%(15/40)上升至全身转移得分6中的100%(1/1),两组比较差异具有统计学意义(P<0.001)。血清高CEA水平的比例从LN-调整全身转移得分1分中占25.0%(2/8)上升至LN-调整全身转移得分7分中的100%(1/1)(P<0.001)。单因素分析结果表明,

  18. 新疆地区汉族、维吾尔族晚期非小细胞肺癌患者血清中CEA表达的差异性研究%Expression of CEA levels in advanced non-small cell lung cancer of the Uygur and Han people in Xinjiang

    Institute of Scientific and Technical Information of China (English)

    马蕾; 徐蓉; 柳江

    2012-01-01

    Objective:To investigate the difference of CEA expression in serum between Uygur and Han patients of advanced non - small cell lung cancer in advanced non - small cell lung cancer. Methods: There were 101 cases of advanced non - small cell lung cancer in the People's Hospital of Autonomous Region in recent 5 years. Among them, there were 50 cases of Uygur and 51 cases of Han patients. We detected the CEA in serum by chemiluminescent enzyme immunoassay, then compared the patients above with 50 healthy Han people and 50 healthy Uygur people in the medical examination center of our hospital. Results: The difference of CEA reached statistical significance between the Han healthy group and the group of advanced non - small cell lung cancer( P 0.05 ) ; Neither did the difference of CEA in the stage IV( P > 0.05 ) ; The difference of CEA failed to reach statistical significance between the Uygur people and Han people in the group of advanced lung squamouse - cell cancer( P > 0. 05); Neither did the difference of CEA in the group of advanced lung non - squa-mouse - cell cancer(P > 0. 05 ). Conclusion: The difference of the expression of CEA reached statistical significance for the Han people and the advanced non - small cell lung cancer patients. ; Neither did the difference of CEA the Uygur healthy group and the group of advanced non - small cell lung cancer. There was no difference of the expression CEA between the Uygur people and Han people in the group of stage Ⅲ and Ⅳ non - small cell lung cancer. There was no difference of the expression of CEA between the Uygur people and Han people in the advanced lung squamouse - cell cancer and the advanced lung non - squamouse - cell cancer.%目的:通过对晚期非小细胞肺癌患者血清中CEA的监测,探讨在维吾尔族、汉族晚期非小细胞肺癌患者之间其表达是否有差异性.方法:新疆维吾尔自治区人民医院2005年5月-2010年5月确诊的晚期非小细胞肺癌患者101

  19. Teratoma during Pregnancy with Positive Estrogen and Progesterone Receptors and Elevated Ca19-9 Antigen Levels

    Directory of Open Access Journals (Sweden)

    Nilgun Kanlioglu Kuman

    2012-01-01

    Full Text Available We present a 27-year-old female patient admitted with an anterior mediastinal mass. She complained of chest discomfort and hemoptysis which began seven months prior. She had given birth five months prior. Thoracic X-ray showed an anterior mediastinal mass. Thorax computed tomography (CT confirmed a well-defined anterior mediastinal mass with  cm diameter, extending to the right hemithorax. It was composed of cystic spaces and discrete areas like soft tissue and fat. Serum Ca 19-9 level was elevated. CT features were consistent with a mature teratoma. During median sternotomy, the tumor revealed adhesions to the right lung and the right subclavian artery. Histologically, the tumor was diagnosed as a mature teratoma. Estrogen and progesterone receptors were detected to be positive in the resected tissue. We conclude that alterations in hormone levels during pregnancy might be the cause of rapid tumor growth which leads to hemoptysis.

  20. Inapplicability of CEA-7 as a canine bone marrow transplantation marker

    International Nuclear Information System (INIS)

    Procedures are described for histocompatibility matching, erythrocyte typing, and CEA-7 serum substance assay. Results of CEA-7 serum substance assays in CEA-7 positive recipients are shown by means of a graph. After lethal total body irradiation, the CEA-7 substance was demonstrable in the recipients' serum for only 7 to 10 days, with disappearance of the substance from the serum for an equivalent period of time and its reappearance thereafter

  1. The part of CEA as informations emitting organism towards the elected circle

    International Nuclear Information System (INIS)

    This document deals with the CEA (Commissariat a l'Energie Atomique) as an organisation emitting information towards politicians. The CEA is a public, scientific and industrial institution. Its main mission is to develop the mastery of the atom in the field of research, health, energy and defense. Lots of political bodies have some contacts with the CEA. A good communication between politicians and the CEA is therefore a must, as it is explained in this document. (TEC)

  2. Genome-wide association replicates the association of Duffy antigen receptor for chemokines (DARC) polymorphisms with serum monocyte chemoattractant protein-1 (MCP-1) levels in Hispanic children.

    Science.gov (United States)

    Voruganti, V Saroja; Laston, Sandra; Haack, Karin; Mehta, Nitesh R; Smith, C Wayne; Cole, Shelley A; Butte, Nancy F; Comuzzie, Anthony G

    2012-12-01

    Obesity is associated with a chronic low inflammatory state characterized by elevated levels of chemokines. Monocyte chemoattractant protein-1 (MCP-1) is a member of the cysteine-cysteine (CC) chemokine family and is increased in obesity. The purpose of this study was to identify loci regulating serum MCP-1 in obese Hispanic children from the Viva La Familia Study. A genome-wide association (GWA) analysis was performed in 815 children, ages 4-19 years, using genotypes assayed with the Illumina HumanOmni1-Quad v1.0 BeadChips. All analyses were performed in SOLAR using a linear regression-based test under an additive model of allelic effect, while accounting for the relatedness of family members via a kinship variance component. The strongest association for MCP-1 levels was found with a non-synonymous single nucleotide polymorphism (SNP), rs12075, resulting in an amino acid substitution (Asp42Gly) in the Duffy antigen receptor for chemokines (DARC) gene product (minor allele frequency=43.6%, p=1.3 × 10(-21)) on chromosome 1. Four other DARC SNPs were also significantly associated with MCP-1 levels (p<10(-16)-10(-6)). The Asp42Gly variant was associated with higher levels of MCP-1 and accounted for approximately 10% of its variability. In addition, MCP-1 levels were significantly associated with SNPs in chemokine receptor 3 (CCR3) and caspase recruitment domain family, member 9 (CARD9). In summary, the association of the DARC Asp42Gly variant with MCP-1 levels replicates previous GWA results substantiating a potential role for DARC in the regulation of pro-inflammatory cytokines. PMID:23017229

  3. Carcinoembryonic Antigen Expression and Resistance to Radiation and 5-Fluorouracil-Induced Apoptosis and Autophagy.

    Science.gov (United States)

    Eftekhar, Ebrahim; Jaberie, Hajar; Naghibalhossaini, Fakhraddin

    2016-01-01

    Understanding the mechanism of tumor resistance is critical for cancer therapy. In this study, we investigated the effect of carcinoembryonic antigen (CEA) overexpression on UV-and 5-fluorouracil (5-FU)-induced apoptosis and autophagy in colorectal cancer cells. We used histone deacetylase (HDAC) inhibitor, NaB and DNA demethylating agent, 5-azacytidine (5-AZA) to induce CEA expression in HT29/219 and SW742 colorectal cancer cell lines. MTT assay was used to measure IC50 value of the cells exposed to graded concentrations of 5- FU with either 0.1 mM NaB or 1 μM 5-AZA for 72 h . Using CHO- and SW742-CEA transfectants, we also investigated the effect of CEA expression on UV- and 5-FU-induced apoptosis and autophagy. Treatment of HT29/219 cell line with NaB and 5-AZA increased CEA expression by 29% and 31%, respectively. Compared with control cells, the IC50 value for 5-FU of NaB and 5-AZA-treated cells increased by 40% and 57%, respectively. Treatment of SW742 cells with NaB or 5-AZA increased neither CEA expression nor the IC50 value for 5-FU. In comparison to parental cells, CEA expression also significantly protected transfected cells against UV-induced apoptosis. Decreased proportions of autophagy and apoptosis were also observed in 5-FU treated SW742- and CHO-CEA transfectants. We conclude that CEA expression can effectively protect colorectal cancer cells against radiation and drug-induced apoptosis and autophagy. PMID:27478804

  4. Tumour-associated trypsin inhibitor (TATI) in human ovarian cyst fluid. Comparison with CA 125 and CEA.

    OpenAIRE

    Halila, H.; Huhtala, M. L.; Haglund, C.; Nordling, S.; Stenman, U. H.

    1987-01-01

    The levels of tumour-associated trypsin inhibitor (TATI), CA 125 and CEA were measured in ovarian cyst fluids from 21 patients. TATI in cyst fluid was immunologically and physicochemically similar to the peptide originally isolated from the urine of a patient with ovarian cancer. Mucinous cysts contained significantly higher levels of TATI than did serous cysts. Immunohistochemically TATI was localized in the apical parts of cells of mucinous ovarian cysts. These results suggest that this tum...

  5. Commercial bacterins did not induce detectable levels of antibodies in mice against Mycoplasma hyopneumoniae antigens strongly recognized by swine immune system

    Directory of Open Access Journals (Sweden)

    Andressa Fisch

    2016-01-01

    Full Text Available Enzootic Pneumonia (EP caused by Mycoplasma hyopneumoniae results in major economic losses to the swine industry. Hence, the identification of factors that provide protection against EP could help to develop effective vaccines. One such factor that provides partial protection are bacterins. Therefore, the aim of this study was to verify the induction of antibodies against fifteen M. hyopneumoniae antigens, strongly recognized by the swine immune system during natural infection, in mice vaccinated with six commercial bacterins. Each group of mice was inoculated with one bacterin, and seroconversion was assessed by indirect ELISA using recombinant antigens and M. hyopneumoniae 7448 whole cell extract. Sera from one inoculated group recognized antigen MHP_0067, and sera from four inoculated groups recognized antigens MHP_0513 and MHP_0580. None of the bacterins was able to induce seroconversion against the twelve remaining antigens. This absence of a serological response could be attributed to the lack of antigen expression in M. hyopneumoniae strains used in bacterin production. Additionally the partial protection provided by these vaccines could be due to low expression or misfolding of antigens during vaccine preparation. Therefore, the supplementation of bacterins with these recombinant antigens could be a potential alternative in the development of more effective vaccines.

  6. Investigating the Effects of Regular Resistance Training and Prostatic Massage on Proinflammatory Markers and Serum Prostate-Specific Antigen Levels in Males with Prostate Cancer

    Directory of Open Access Journals (Sweden)

    Fathollahi Shoorabeh

    2016-01-01

    Full Text Available Background Prostate cancer (PC is one of the most common cancers worldwide. Some studies support that chronic inflammation of prostate tissue plays a role in the development of PC. A variety of growth factors and cytokines may lead to proinflammatory processes within the prostate. Objectives The aim of the present study was to investigate the effects of eight weeks of regular resistance training and prostatic massage on proinflammatory markers CRP, IL-6, TNF-α, and IL-10 and serum prostate-specific antigen (PSA levels in males with PC. Patients and Methods Forty-five patients with PC were selected for this study. They were randomized into either the resistance training intervention group (n = 15, the massage intervention group (n = 15, or the control group (n = 15. Resistance-training patients participated in resistance training for eight weeks, and massage was performed for six weeks on the massage group. Repeated measures analysis of variance (ANOVA was used to analyze the data (P ≤ 0.05. Results In the resistance training group, IL-10 levels significantly increased after four (P = 0.055 and eight weeks (P = 0.000. Four and eight weeks of resistance training showed a significant reduction in PSA, CRP, IL-6, and TNF-α levels (P < 0.05. Patients of massage intervention showed an increase in IL-10 after four (P = 0.045 and six weeks (P = 0.005. In addition, four and six weeks of massage intervention showed a significant reduction in PSA, CRP, IL-6, and TNF-α levels (P < 0.05. Conclusions Regular resistance training and prostatic massage can improve proinflammatory markers and PSA levels in men with PC.

  7. Prostate-specific antigen levels are higher in African-American than in white patients in a multicenter registration study: Results of RTOG 94-12

    International Nuclear Information System (INIS)

    Purpose: To compare serum prostate-specific antigen (PSA) levels in a national sample of African-American and white men with prostate cancer, and to attempt to explain any differences by using self-reported individual-level socioeconomic status adjustments. Methods and Materials: During 4((1)/(2)) months in 1994-95, 709 patients with nonmetastatic prostate cancer were enrolled in this prospective study; 17.5% were African-American and 82.5% were white. Information about clinical stage, tumor grade, pretreatment PSA, type of insurance, and educational and income status was obtained. Serum PSA levels were measured and racial differences were found; how the differences were influenced by other patient- or tumor-related factors and if the differences could be explained by socioeconomic status disparities were determined. In univariate analyses, factors associated with the mean PSA levels were studied; log-converted values were used to yield a normal distribution. Multivariate analyses were done on log-linear models for description of association patterns among various categorical variables; a perfectly fitted model should have a correlation value (CV) of 1.0. Results: The mean PSA level was higher in African-Americans (14.68 ng/ml) than in whites (9.82 ng/ml) (p = 0.001). Clinical stage (p = 0.001), Gleason sum tumor grade (p = 0.0001), educational level (p = 0.001), and household income (p = 0.03) were also associated with mean PSA levels; age, type of biopsy, and insurance status were not. Disease stage (p = 0.0001), grade (p 0.0001), education (p = 0.07), and income (p = 0.02) were all associated with PSA levels for whites, but none of these factors were important for African-Americans (all p values > 0.1). The best fitted log-linear model (CV = 0.99) contained PSA ( 20), Gleason sum grade (2-5, 6-7, and 8-10), race, and two interactions: PSA by race (p = 0.0012) and PSA by Gleason sum (p = 0.0001). Models replacing race for either income (CV = 0.82) or education

  8. Evaluation of chemotherapy response with serum squamous cell carcinoma antigen level in cervical cancer patients: a prospective cohort study.

    Directory of Open Access Journals (Sweden)

    Mingzhu Yin

    Full Text Available MRI does not always reflect tumor response after chemotherapy. Therefore, it is necessary to explore additional parameters to more accurately evaluate tumor response for the subsequent clinical determination about radiotherapy or radical surgery. A training cohort and an external validation cohort were used to examine the predictive performance of SCC-ag to evaluate tumor response from teaching hospital of Harbin Medical University. The study included 397 women with SCC (age: 28-73 years. Patients consecutively enrolled between August 2008 and January 2010 (n = 205 were used as training cohort. Patients consecutively enrolled between February 2010 and May 2011 (n = 192 were used as validation cohort. A multivariate regression analysis of the data from the training cohort indicated that serum SCC-ag level is an independent factor for neo-adjuvant chemotherapy (NACT response. Analysis of the data from the validation cohort suggested that chemotherapy response could be more accurately predicted by SCC-ag than by magnetic resonance imaging (MRI (sensitivity (Se: 0.944 vs. 0.794; specificity (Sp: 0.727 vs. 0.636; positive predictive value (PPV: 0.869 vs. 0.806; negative predictive value (NPV: 0.873 vs. 0.618; the area under ROC curve (AUC: 0.898 vs. 0.734. Combining SCC-ag with MRI was more powerful than MRI alone (Se: 0.952 vs. 0.794; Sp: 0.833 vs. 0.636; PPV: 0.916 vs. 0.806; NPV: 0.902 vs. 0.618; AUC: 0.950 vs. 0.734. Our study indicates that serum SCC-ag level is a sensitive and reliable measure to evaluate cervical cancer response to chemotherapy. Using SCC-ag in combination with MRI findings further improves the predictive power.

  9. Prostate cancer detection upon transrectal ultrasound-guided biopsy in relation to digital rectal examination and prostate-specific antigen level: what to expect in the Chinese population?

    Directory of Open Access Journals (Sweden)

    Jeremy YC Teoh

    2015-01-01

    Full Text Available We investigated the prostate cancer detection rates upon transrectal ultrasound (TRUS-guided biopsy in relation to digital rectal examination (DRE and prostate-specific antigen (PSA, and risk factors of prostate cancer detection in the Chinese population. Data from all consecutive Chinese men who underwent first TRUS-guided prostate biopsy from year 2000 to 2013 was retrieved from our database. The prostate cancer detection rates with reference to DRE finding and PSA level of 50 ng ml−1 were investigated. Multivariate logistic regression analyses were performed to investigate for potential risk factors of prostate cancer detection. A total of 2606 Chinese men were included. In patients with normal DRE, the cancer detection rates were 8.6%, 13.4%, 21.8%, 41.7% and 85.2% in patients with PSA 50 ng ml−1 respectively. In patients with abnormal DRE, the cancer detection rates were 12.4%, 30.2%, 52.7%, 80.6% and 96.4% in patients with PSA 50 ng ml−1 respectively. Older age, smaller prostate volume, larger number of biopsy cores, presence of abnormal DRE finding and higher PSA level were associated with increased risk of prostate cancer detection upon multivariate logistic regression analyses (P < 0.001. Chinese men appeared to have lower prostate cancer detection rates when compared to the Western population. Taking the different risk factors into account, an individualized approach to the decision of TRUS-guided biopsy can be adopted.

  10. Pretreatment Carbohydrate Antigen 19-9 Level Indicates Tumor Response, Early Distant Metastasis, Overall Survival, and Therapeutic Selection in Localized and Unresectable Pancreatic Cancer

    International Nuclear Information System (INIS)

    Purpose: The use of chemoradiotherapy (CRT) for localized and unresectable pancreatic cancer has been disputed because of high probability of distant metastasis. Thus, we analyzed the effect of clinical parameters on tumor response, early distant metastasis within 3 months (DM3m), and overall survival to identify an indicator for selecting patients who would benefit from CRT. Methods and Materials: This study retrospectively analyzed the data from 84 patients with localized and unresectable pancreatic cancer who underwent CRT between August 2002 and October 2009. Sex, age, tumor size, histological differentiation, N classification, pre- and post-treatment carbohydrate antigen (CA) 19-9 level, and CA 19-9 percent decrease were analyzed to identify risk factors associated with tumor response, DM3m, and overall survival. Results: For all 84 patients, the median survival time was 12.5 months (range, 2–31.9 months), objective response (complete response or partial response) to CRT was observed in 28 patients (33.3%), and DM3m occurred in 24 patients (28.6%). Multivariate analysis showed that pretreatment CA 19-9 level (≤400 vs. >400 U/ml) was significantly associated with tumor response (45.1% vs. 15.2%), DM3m (19.6% vs. 42.4%), and median overall survival time (15.1 vs. 9.7 months) (p 3m, and overall survival and identifying patients who will benefit from CRT.

  11. Simultaneous Transurethral Resection of Prostate and Prostate Needle Biopsy in Patients with Acute Urinary Retention and Elevated Prostate Specific Antigen Levels

    Directory of Open Access Journals (Sweden)

    Yen-Ta Chen

    2009-08-01

    Full Text Available Background: To evaluate the safety and accuracy of simultaneous transrectal ultrasonography (TRUS-guided prostate biopsy and transurethral resection of prostate(TURP, patients with acute urinary retention (AUR who underwent simultaneous prostate biopsy and TURP were compared with those treated byTURP alone.Methods: From July 2000 to Jun 2004, 21 patients older than 70 years with AUR wereincluded in each group. Patients with elevated prostate specific antigen(PSA levels (> 4 ng/ml underwent TURP and TRUS-guided biopsy simultaneously (group I, while those with normal PSA levels (< 4 ng/ml receivedTURP alone (group II. The average ages of group I and group II patientswere 72.4 and 72.5 years.Results: In group I, 4 patients (19% were diagnosed with prostate cancer; 2 hadmetastasis and received hormone therapy; and 1 had localized cancer andwas treated with brachytherapy. An 82-year-old patient with localized canceropted for watchful waiting. Compared to group II, patients in group I did notshow aggregated morbidity, such as prolonged hospital stay, hematuria, orfever.Conclusion: Simultaneous TURP and TRUS-guided biopsy in healthy AUR patients doesnot increase the risk of morbidity. Furthermore, with this approach patientscan recover from the stress of AUR and have a definite diagnosis of theircondition.

  12. Evaluation of the nuclear installations safety of the CEA in 1998; Le bilan de la surete des installations nucleaires du CEA en 1998

    Energy Technology Data Exchange (ETDEWEB)

    Laverie, M. [CEA, Dir. de la Surete Nucleaire et de la Qualite, 75 - Paris (France)

    1999-09-01

    Michel Laverie, Director of the nuclear safety and quality at the Cea, took stoke of the CEA nuclear installations in 1998. After a recall of the nuclear safety policy and organization, the author presents the risks factors bound to the CEA activities as the dismantling, the wastes and the human factors. A last part is devoted to the list of the accidents occurred during 1998 in the nuclear installations. Tables and statistics illustrate this analysis. (A.L.B.)

  13. Supercritical fluids: multiplication of projects at Cea

    International Nuclear Information System (INIS)

    Supercritical fluids have different applications: To clear polluted lands by use of supercritical carbon dioxide, to use supercritical water to oxide low level radioactive effluents, (a project is to be studied to treat effluents and to protect reactors against corrosion); by adding supercritical fluid to a viscous fluid it is possible to reduce the viscosity and then to use filtration; for this reason projects are studied to couple the two processes to filter of spent oils and clear them of some pollutants. (N.C.)

  14. Annual report of the Association EURATOM/Cea

    International Nuclear Information System (INIS)

    This annual report presents research activities, which have been performed in 2002 by the French EURATOM-Cea association in the frame of the European technology program. The first section describes EFDA (European fusion development agreement) activities and related developments carried out by the association. The second one is dedicated to the underlying technology program and finally the third one presents the inertial confinement fusion activities. In each section the tasks are sorted out according to the EFDA main fields: physics (heating and current drive, remote participation, diagnostics), vessel/in-vessel (vessel/blanket, plasma facing components, remote handling), magnet, tritium breeding and materials (water cooled lithium lead blanket, helium cooled pebble bed blanket, helium cooled lithium lead blanket, reduced activation ferritic martensitic steels, advanced materials, neutron source, fuel cycle), safety and environment, system studies (power plant conceptual studies, socio-economic studies) and JET technology activities. The EURATOM-Cea association is involved in all these studies

  15. 2011 reporting of the risk handling at CEA

    International Nuclear Information System (INIS)

    In 2011 CEA reported 100 events to the Authority for Nuclear Safety (ASN). 93 were graded 0 on the INES scale and 7 were graded 1. None of them had a significant impact on the staff health and the environment. 25% of these events concerned delays in the controls of safety equipment. In 2011 CEA had to manage several situations that led to a response of its crisis center: 1) the explosion of an oven belonging to the Socodei-Centraco company situated near the Marcoule Center, 2) the discovery of about 500 grenades from the first world war during digging out works in the Grenoble Center, 3) flooding due to heavy rains in the Cadarache Center, and 4) an intrusion attempt at the Cadarache Center. (A.C.)

  16. Overview of CEA research in the field of radionuclides migration

    International Nuclear Information System (INIS)

    This report presents a synthetic status of the researches conducted within the Nuclear Energy Division (CEA/DEN) in the field of radionuclides migration in three specific areas which have been chosen for their representativeness and potential impact: the migration of RN in PWR reactors, the migration of RN from a deep geological repository and the migration processes in the surface environments. In addition, some status is given about more generic research which is conducted in the field of RN speciation in the aqueous phase and at the interfaces and regarding chemistry / transport couplings. Additional information about the human and technical means involved in these fields of research in CEA/DEN is finally given in the Appendix. (authors)

  17. Physical protection of nuclear materials and facilities in CEA

    International Nuclear Information System (INIS)

    Full text: CEA (Commissariat a l'Energie Atomique), as nuclear operator, is responsible for the control and protection of their nuclear materials. Inside CEA, DCS (Central Security Division) is in charge of the security matters, DCS defines the CEA strategy in this field, especially in physical protection. The paper will present the physical protection strategy of CEA. DCS defines the rules and methods; the operators have to apply in order to fulfill the security objectives of CEA. CEA has to provide the regulatory authority with documents proving that it is in accordance with the requirements of the 25th July 1980 law and 12th May 1981 decree. It has to implement all the necessary means in order to achieve the results requested by the regulatory authority. All these arrangements are described in the 'license and control file'. This file should specify the facility safeguards and physical protection system. Accounting measures are also described. In this file, the petitioner has to justify its capacity for holding nuclear materials and for exercising authorized activities on them. So the organization and the installed means have to be described in this authorization file. For physical protection, containment, surveillance and physical protection measures are presented: Containment measures must prevent the unauthorized or unjustified movements of nuclear material in the framework of the authorized activities; Surveillance measures must guarantee the integrity of the containment, check that no material is exiting by an abnormal channel; Physical protection measures for the materials, the premises and the facilities are intended to protect them against malevolent actions by means of security systems. The Central Security Division has established guidelines to provide guidance to the nuclear materials holders in writing such files. Each holding unit has to establish a 'license and control file' and each CEA site establishes a 'site license and control file

  18. Physical protection of nuclear materials and facilities in CEA

    International Nuclear Information System (INIS)

    CEA (Commissariat a l'Energie Atomique), as nuclear operator, is responsible for the control and protection of their nuclear materials. Inside CEA, DCS (Central Security Division) is in charge of the security matters, DCS defines the CEA strategy in this field, especially in physical protection. The paper will present the physical protection strategy of CEA. DCS defines the rules and methods, the operators have to apply in order to fulfill the security objectives of CEA. CEA has to provide the regulatory authority with documents proving that it is in accordance with the requirements of the 25th July 1980 law and 12th May 1981 decree. It has to implement all the necessary means in order to achieve the results requested by the regulatory authority. All these arrangements are described in the 'license and control file'. This file should specify the facility safeguards and physical protection system. Accounting measures are also described. In this file, the petitioner has to justify its capacity for holding nuclear materials and for exercising authorized activities on them. So the organization and the installed means have to be described in this authorization file. For physical protection, containment, surveillance and physical protection measures are presented: Containment measures must prevent the unauthorized or unjustified movements of nuclear material in the framework of the authorized activities; Surveillance measures must guarantee the integrity of the containment, check that no material is exiting by an abnormal channel; Physical protection measures for the materials, the premises and the facilities are intended to protect them against malevolent actions by means of security systems. The Central Security Division has established guidelines to provide guidance to the nuclear materials holders in writing such files. Each holding unit has to establish a 'license and control file' and each CEA site establishes a 'site license and control file'. Guidelines have been

  19. Annual report of the Association EURATOM/Cea

    Energy Technology Data Exchange (ETDEWEB)

    Magaud, Ph.; Le Vagueres, F

    2002-07-01

    This annual report presents research activities, which have been performed in 2002 by the French EURATOM-Cea association in the frame of the European technology program. The first section describes EFDA (European fusion development agreement) activities and related developments carried out by the association. The second one is dedicated to the underlying technology program and finally the third one presents the inertial confinement fusion activities. In each section the tasks are sorted out according to the EFDA main fields: physics (heating and current drive, remote participation, diagnostics), vessel/in-vessel (vessel/blanket, plasma facing components, remote handling), magnet, tritium breeding and materials (water cooled lithium lead blanket, helium cooled pebble bed blanket, helium cooled lithium lead blanket, reduced activation ferritic martensitic steels, advanced materials, neutron source, fuel cycle), safety and environment, system studies (power plant conceptual studies, socio-economic studies) and JET technology activities. The EURATOM-Cea association is involved in all these studies.

  20. 50. anniversary of the CEA. Anniversary Symposium papers

    International Nuclear Information System (INIS)

    This conference is composed of three tomes, and the titles of the various sessions are: fifty years of nuclear history; nuclear connection balance; uranium enrichment; nuclear wastes: past and present; nuclear fuel reprocessing; nuclear safety; research reactors at the CEA; birth of a scientific discipline, neutronics; fusion through magnetic confinement; large machines for the infinitesimal; laser, a marvelous tool; exploration of condensed matter; knowledge of the atomic nucleus; invariances and symmetries; nuclear astrophysics; polymers and interfaces; micro-electronics and micro-technologies; prospective with nano-technologies; metallurgy and materials; modelization and numerical simulation; electromagnetism and stealth; CEA, chemistry, health and environment; climate and environment, our past and future; the Earth from its formation to Man; life and nuclear tools; photosynthesis, how living beings use solar energy

  1. The CEA and alternative energies. 8 April 2010 press conference

    International Nuclear Information System (INIS)

    This document presents the CEA's strategy in terms of alternative energies and the various implemented research programs which mainly concern the building sector and the transport sector. After a recall of the energy and climate context, a presentation of the NTE program (Nouvelles Technologies de l'Energie, new energy technologies), the different topics and projects are presented: photovoltaic solar energy and its integration in building; batteries, hydrogen and fuel cells for applications in transports; second-generation bio-fuels

  2. Facilities offered by the CEA group for training foreign specialists

    International Nuclear Information System (INIS)

    First set up in 1945, the CEA (French Atomic Energy Commission) opened its doors to foreign scientists and technicians as long ago as the 1950s, and particularly so since 1960. Among the 15 000 foreigners concerned, some have come in the capacity of co-workers, while others - approximately 10 000 - have taken courses for the purpose of receiving training. Since the CEA constitutes both a public scientific institution and an industrial group, especially by virtue of its branch organizations and specialized production units, it possesses incomparably varied facilities. This is why almost all the countries of the world, at some time or other, have had nationals at the Saclay, Fontenay-aux-Roses, Grenoble and Cadarache research centres, or the National Institute of Nuclear Science and Technology (INSTN) at Saclay, a specialized establishment set up in 1956, or else at the Razes Information and Further Training Centre (CIPRA), specializing in the uranium ore industry. Most of the trainees received over the last few years by the CEA Group stem from the developing countries, those coming to receive practical technological training being ever more numerous. Several of the training programmes devised have involved participation in industrial operations, either in conjunction with Technicatome, a branch organization specializing in nuclear engineering (Iran, Iraq) or in association with other CEA Group units (South Korea). The trainees usually receive fellowships through the intermediary of French diplomatic missions, in which case it is enough if they continue to receive the salary normally paid to them by the public organization employing them. On the other hand, technological training sometimes involves payment because of the expense involved (intensive supervision and expensive experimental work). Finally, courses in French are organized in France by specialized institutions, but English is acceptable for very brief training periods. (author)

  3. RELAP4/MOD-5-CEA pump coastdown experiment simulation

    International Nuclear Information System (INIS)

    Since is important the theoretical-experimental comparison to evaluate the computer codes, these paper presents the simulation with RELAP4/MOD5 Code of a loss of power energy in the pump of the ''Circuito Experimental de Agua-CEA''. From the results attained, the existing models in the Code showed to be very satisfatory quantitative and qualitative behavior of the attained experimental results. (author)

  4. Report transparency and nuclear safety 2007 CEA Cadarache

    International Nuclear Information System (INIS)

    This report presents the activities of the CEA Center of Cadarache for the year 2007. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the report discusses the beginning of the RJH reactor construction, the fourth generation reactors research programs, the implementing of la Rotonde the new radioactive wastes management installation, the renovation of the LECA. (A.L.B.)

  5. Report transparency and nuclear safety 2007 CEA Marcoule

    International Nuclear Information System (INIS)

    This report presents the activities of the CEA Center of Marcoule for the year 2007. Since its creation in 1955 the center realizes industrial and scientific activities relative to the civil and military applications of the radioactivity. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the following two base activities are detailed: Atalante and Phenix. (A.L.B.)

  6. Fire fight and security in CEA's research centers

    International Nuclear Information System (INIS)

    In each CEA's center a special unit called FLS (formation locale de securite) is in charge of the protection of staff and property. This unit is polyvalent and qualified for fire fight, first aid and guarding, its members are recruited mainly among the Paris fire brigade and the Marseilles' brigade of sea firemen. Their role is to prevent and detect any risk whatever this risk may be: chemical, electric or nuclear. (A.C.)

  7. Report transparency and nuclear safety 2007 CEA Saclay

    International Nuclear Information System (INIS)

    This report presents the activities of the CEA Center of Saclay for the year 2007. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially two public consultation on release authorizations and the Neurospin installations, the dismantling of the 49 nuclear installation, the shutdown of the learning reactor ULYSSE are detailed. (A.L.B.)

  8. Radiotherapy options for localized prostate cancer based upon pretreatment serum prostate-specific antigen levels and biochemical control: A comprehensive review of the literature

    International Nuclear Information System (INIS)

    Purpose: To review all the available radiotherapy (RT) literature on localized prostate cancer treatment where serum prostate-specific antigen (PSA) levels were used to both stratify patients and evaluate outcome and determine if any conclusions can be reached regarding an optimal radiotherapeutic management for this disease. Methods and Materials: A MEDLINE search was conducted to obtain all articles in English on prostate cancer treatment employing RT from 1986-1997. Studies were considered eligible for review only if they met all the following criteria: 1) pretreatment PSA values were recorded and grouped for subsequent evaluation, 2) posttreatment PSA values were continuously monitored, 3) definitions of biochemical control were stated, and 4) the median follow-up was given. Results: Of the 246 articles identified, only 20 met the inclusion criteria; 4 using conformal external beam RT, 8 using conventional external beam RT, and 8 using interstitial brachytherapy (4 using a permanent implant alone, 3 combining external beam RT with a permanent implant, and 1 combining a conformal temporary interstitial implant boost with external beam RT). No studies using neutrons (with or without external beam RT) or androgen deprivation (combined with external beam RT) were identified where patients were stratified by pretreatment PSA levels. Results for all therapies were extremely variable with the 3-5-year rates of biochemical control for patients with pretreatment PSA levels ≤4 ng/ml ranging from 48 to 100%, for PSA levels >4 and ≤10 ng/ml ranging from 44 to 90%, for PSA levels >10 and ≤20 ng/ml ranging from 27 to 89%, and for PSA levels >20 ranging from 14 to 89%. The median Gleason score, T-stage, definition of biochemical control, and follow-up were substantially different from series to series. No RT option consistently produced superior results. Conclusions: When data are reviewed from studies using serum PSA levels to stratify patients and to evaluate

  9. CEA's research programmes on future nuclear power systems

    International Nuclear Information System (INIS)

    The CEA (Commissariat a l'energie atomique et aux energies alternatives) is at the crossroads between sciences and technologies. In collaboration with corporate leaders and academics, it is working to improve the competitiveness of the existing fleet of nuclear facilities (PWR reactors and EPR) and to develop both a fourth generation of nuclear power reactors and technologies for processing spent fuel. Concerning the PWR reactors most of the work focuses on the extension of reactor operating life (till 60 years), a better use of uranium fuel in the reactor core, a better safety standard, a better reliability of fuel assemblies. This work is both theoretical and experimental with the possibility to use irradiation rigs at the OSIRIS reactor. Concerning the EPR most of the work is dedicated to sustain the commissioning and start of the first EPR in France on the Flamanville site. Concerning the fuel cycle, CEA's teams are working to improve every stage of the cycle from uranium extraction to the processing of spent fuels. In the nuclear industry, industrial cycles are very long so it is today's work to prepare the fourth generation of reactors even if the first reactor of the third generation is not yet in operation. CEA's research focuses on 2 types of fast reactors, one is sodium-cooled (with ASTRID prototype) while the other is gas-cooled (with ALLEGRO prototype). (A.C.)

  10. Lessons learned from CEA experience with dismantling project

    International Nuclear Information System (INIS)

    Since the late 1980s the CEA has been actively implementing methods to benefit from its experience in dismantling nuclear facilities. Capitalizing on prior experience - originally to address safety requirements - has now acquired greater importance in the CEA with the implementation of project management knowledge bases that require better control of performance and cost from initial estimates to completion. Changing regulations (waste, radiological protection, safety, etc.) and decommissioning policies in major organizations (objectives, funding, deadlines, organization, etc.) significantly influence dismantling projects. Experience with knowledge management has shown that the approach consisting in exhaustive project data collection often produces large, unwieldy information bases that are difficult to apply to subsequent projects. Principles and recommendations can nevertheless be derived, but require detailed analysis of all the aspects of dismantling (waste, dosimetry, conventional and nuclear safety, cost, deadlines, physical and radiological environment, regulatory provisions, etc.). The wide range of CEA facilities, the different stages in the course of a project that often lasts for several decades, changing operators and supervisors, all imply that a given situation can be assessed and reconsidered differently depending on the project organization, participants and background. Past results can be considered technically satisfactory, but poorly rated in terms of cost or scheduling. The current approach consists in focusing more on the relevance of the information conserved, favoring common practices emanating from a dedicated center of expertise, and applying suitable processes after discriminating between the lessons learned directly from experience and from consolidation of professional knowledge bases. (author)

  11. Elevated IgG levels against specific bacterial antigens in obese patients with diabetes and in mice with diet-induced obesity and glucose intolerance.

    Science.gov (United States)

    Mohammed, Nadeem; Tang, Lihua; Jahangiri, Anisa; de Villiers, Willem; Eckhardt, Erik

    2012-09-01

    High fat diets increase the risk for insulin resistance by promoting inflammation. The cause of inflammation is unclear, but germfree mouse studies have implicated commensal gut bacteria. We tested whether diet-induced obesity, diabetes, and inflammation are associated with anti-bacterial IgG. Blood from lean and obese healthy volunteers or obese patients with diabetes were analyzed by ELISA for IgG against extracts of potentially pathogenic and pro-biotic strains of Escherichia coli (LF-82 and Nissle), Bacteroides thetaiotaomicron, and Lactobacillus acidophilus, and for circulating tumor necrosis factor α (TNFα). C57Bl/6 mice were fed low- or high-fat diets (10% or 60% kcal from fat) for 10 weeks and tested for anti-bacterial IgG, bodyweight, fasting glucose, and inflammation. Obese diabetic patients had significantly more IgG against extracts of E. coli LF-82 compared with lean controls, whereas IgG against extracts of the other bacteria was unchanged. Circulating TNFα was elevated and correlated with IgG against the LF-82 extract. Mice fed high-fat diets had increased fasting glucose levels, elevated TNFα and neutrophils, and significantly more IgG against the LF-82 extracts. Diabetes in obesity is characterized by increased IgG against specific bacterial antigens. Specific commensal bacteria may mediate inflammatory effects of high-fat diets. PMID:22424821

  12. Evaluation of Serum Testosterone and Prostate Specific Antigen Levels in Sprague-Dawley Female Rats Bearing Mammary Tumours Induced by Dimethylbenz (A) Anthracene

    International Nuclear Information System (INIS)

    Breast Cancer is one of the most common malignancies among women in the world and is a leading cause of morbidity and mortality in females of both developed and developing countries. The present work aimed to study the diagnostic value of prostate specific antigen (PSA) for breast cancer and the importance of treatment by blue green algae Spirulina platensis supplementation on PSA and testosterone levels. Two different analytical techniques based on micro particle enzyme immunoassay (MPIA) and enzyme linked fluorescent assay (ILFA) for quantitative determination of total PSA (free PSA and PSA complexed to alpha-1-antichymptrypsin) were studied in the diagnosis of breast cancer before and after Spirulina platensis supplementation. Female rats secret PSA from female prostate gland, serum testosterone and PSA concentrations were not detected in control and rats supplemented by Spirulina platensis groups. Serum testosterone and PSA concentration were significantly higher (P< 0.05) in rats bearing mammary tumours than rats bearing mammary tumours supplemented with Spirulina platensis. These results suggest that the clinical applicability of total PSA for breast cancer diagnosis and the biological mechanism behind its increase should be further investigated. Finally, the physiological mechanism behind the total PSA increase in breast cancer and its ramifications with respect to tumour progression should be further investigated.

  13. Fitoplancton del Parque Nacional Las Tablas de Daimiel. II. Las cianofíceas, los dinoflagelados, las criptofíceas, las crisofíceas y las xantofíceas

    Directory of Open Access Journals (Sweden)

    Rojo, Carmen

    1999-12-01

    Full Text Available Las Tablas de Daimiel National Park is a semiarid wetland that exhibits a high biodiversity but is suffering an inexorable eutrophication process. The study of the phytoplankton in this wetland is interesting because of its hydrological fluctuations (drought period until 1996 and its trophic condition. This papers shows the taxonomy, dynamic, and ecology of microalgae in five áreas of the Park from 1996 to 1998, which allow comparisons to be made between the present state and the 1992-1993 period. Twelve taxa of Cyanophyta, 4 of Dynophyceae, 8 of Cryptophyceae, 5 of Chrysophyceae and one of Xanthophyceae were found. Almost all species have been already cited in Spain and are typical for eutrophic environments. Cyanophyceae and Cryptophyceae were the most important groups (Planktothrix agardhii, Anabaenopsis elenkinii, Cryptomonas erosa as the most common algae. Moreover, oscillatorial Cyanophyceae (in 1992 have been replaced by N-fixing nostocal ones, related to the high level of pollution found in recent years.El Parque Nacional Las Tablas de Daimiel es un humedal semiárido, importante reserva de biodiversidad que, sin embargo, está sufriendo un inexorable proceso de eutrofización. Su estado trófico, así como los cambios hidrológicos (sequía hasta 1996, hacen especialmente interesante el estudio de su microflora. Por ello, se ha llevado a cabo este trabajo sobre las poblaciones fitoplanctónicas en cinco puntos del Parque desde 1996 a 1998, que permite además la comparación con su estado en 1992-1993. Se encontraron 12 táxones de Cyanophyta, 4 de Dynophyceae, 8 de Cryptophyceae, 5 de Chrysophyceae y uno de Xanthophyceae. Casi todas las especies están citadas en España y son propias de ambientes eutróficos. Los grupos mejor representados fueron las cianobacterias y criptofíceas (Planktothrix agardhii, Anabaenopsis elenkinii y Cryptomonas erosa fueron las especies más conspicuas. Además, se observa un cambio de cianobacterias

  14. Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

  15. CD66 carcinoembryonic antigens mediate interactions between Opa-expressing Neisseria gonorrhoeae and human polymorphonuclear phagocytes.

    Science.gov (United States)

    Gray-Owen, S D; Dehio, C; Haude, A; Grunert, F; Meyer, T F

    1997-06-16

    Colonization of urogenital tissues by the human pathogen Neisseria gonorrhoeae is characteristically associated with purulent exudates of polymorphonuclear phagocytes (PMNs) containing apparently viable bacteria. Distinct variant forms of the phase-variable opacity-associated (Opa) outer membrane proteins mediate the non-opsonized binding and internalization of N. gonorrhoeae by human PMNs. Using overlay assays and an affinity isolation technique, we demonstrate the direct interaction between Opa52-expressing gonococci and members of the human carcinoembryonic antigen (CEA) family which express the CD66 epitope. Gonococci and recombinant Escherichia coli strains synthesizing Opa52 showed specific binding and internalization by transfected HeLa cell lines expressing the CD66 family members BGP (CD66a), NCA (CD66c), CGM1 (CD66d) and CEA (CD66e), but not that expressing CGM6 (CD66b). Bacterial strains expressing either no opacity protein or the epithelial cell invasion-associated Opa50 do not bind these CEA family members. Consistent with their different receptor specificities, Opa52-mediated interactions could be inhibited by polyclonal anti-CEA sera, while Opa50 binding was instead inhibited by heparin. Using confocal laser scanning microscopy, we observed a marked recruitment of CD66 antigen by Opa52-expressing gonococci on both the transfected cell lines and infected PMNs. These data indicate that members of the CEA family constitute the cellular receptors for the interaction with, and internalization of, N. gonorrhoeae. PMID:9218786

  16. Aptasensor based on tripetalous cadmium sulfide-graphene electrochemiluminescence for the detection of carcinoembryonic antigen.

    Science.gov (United States)

    Shi, Gui-Fang; Cao, Jun-Tao; Zhang, Jing-Jing; Huang, Ke-Jing; Liu, Yan-Ming; Chen, Yong-Hong; Ren, Shu-Wei

    2014-11-21

    A facile label-free electrochemiluminescence (ECL) aptasensor, based on the ECL of cadmium sulfide-graphene (CdS-GR) nanocomposites with peroxydisulfate as the coreactant, was designed for the detection of carcinoembryonic antigen (CEA). Tripetalous CdS-GR nanocomposites were synthesized through a simple onepot solvothermal method and immobilized on the glassy carbon electrode surface. L-Cystine (L-cys) could largely promote the electron transfer and enhance the ECL intensity. Gold nanoparticles (AuNPs) were assembled onto the L-cys film modified electrode for aptamer immobilization and ECL signal amplification. The aptamer modified with thiol was adsorbed onto the surface of the AuNPs through a Au-S bond. Upon hybridization of the aptamer with the target protein, the sequence could conjugate CEA to form a Y architecture. With CEA as a model analyte, the decreased ECL intensity is proportional to the CEA concentration in the range of 0.01-10.0 ng mL(-1) with a detection limit of 3.8 pg mL(-1) (S/N = 3). The prepared aptasensor was applied to the determination of CEA in human serum samples. The recoveries of CEA in the human serum samples were between 85.0% and 109.5%, and the RSD values were no more than 3.4%. PMID:25209409

  17. Targeted sonodynamic therapy of cancer using a photosensitizer conjugated with antibody against carcinoembryonic antigen.

    Science.gov (United States)

    Abe, Hironori; Kuroki, Motomu; Tachibana, Katsuro; Li, Tieli; Awasthi, Aradhana; Ueno, Aruto; Matsumoto, Hisanobu; Imakiire, Takayuki; Yamauchi, Yasushi; Yamada, Hiromi; Ariyoshi, Asami; Kuroki, Masahide

    2002-01-01

    The goal of this study was to develop a strategy for the selective destruction of cancer cells by ultrasonic irradiation in the presence of an antibody-conjugated photosensitizer. To this end, a photoimmunoconjugate (PIC) was prepared between ATX-70, a photosensitizer of a gallium-porphyrin analogue, and F11-39, a high affinity monoclonal antibody (MAb) against carcinoembryonic antigen (CEA), which is often overexpressed in various carcinoma cells. This conjugate, designated F39/ATX-70, retained immunoreactivity against purified CEA and CEA-expressing cells as determined by enzyme-linked immunosorbent assay, flow cytometry and immunofluorescence microscopic analysis. The cytotoxicity of F39/ATX-70 against CEA-expressing human gastric carcinoma cells in vitro was found to be greater than that of ATX-70 when applied in combination with ultrasound irradiation. When in vivo anti-tumor effects in a mouse xenograft model were assessed, intravenous administration of F39/ATX-70 followed by ultrasonic irradiation produced a marked growth inhibition of tumor compared with irradiation alone or irradiation after administration of ATX-70. These results suggest that the PIC between anti-CEA MAb and ATX-70 may have applications in sonodynamic therapy where destruction of CEA-expressing tumor is required. PMID:12168839

  18. AIRIX: an induction accelerator facility developed at CEA for flash radiography in detonics

    Science.gov (United States)

    Cavailler, Claude

    1999-06-01

    AIRIX is an induction linear accelerator which will be used for flash radiography in CEA/DAM. Designed to produce an X-ray dose of more than 500 Rads at 1 meter with an X-ray focal spot size diameter of less than 2 mm (LANL-CEA DAM definition), this facility consists in a 4 MeV/3.5 kA pulsed electron injector and 16 MeV induction accelerator powered by 32 high voltage generators. A prototype of this accelerator, called PIVAIR, has been studied and realized in CEA CESTA near Bordeaux. PIVAIR is a validation step for AIRIX at 8 MeV. It includes an injector (4 MeV, 3.5 kA, 60 ns) and 16 inductor cells supplied by 8 high voltage generators (250 kV, 70 ns). Two different technologies of induction cells have been tested (rexolite insulator or ferrite under vacuum). We have chosen ferrite under vacuum cells technology after comparison of results on beam transport and reliability tests. A focusing experiment at 7.2 MeV of the electron beam as been achieved during summer 1997. We have begun to produce X-rays in October 1997. A dose level of 50 Rad at 1 meter has been achieved with an X-ray spot size diameter of 3.5 to 4 mm (LANL-CEA DAM definition). Static flash radiography of very dense object have been achieved from November 97 until February 98. We have been able to test in situ new kinds of very high sensitive X- ray detectors and to check they had reached our very ambitious goals: (1) quantum efficiency at 5 MeV greater than 50% instead of 1% for luminous screens and film; (2) sensitivity less than 10 (mu) Rad (100 time more sensitive than radiographic luminous screens and films); (3) dynamic range greater than 100; (4) resolution less than 2 mm. We will present in this communication brand new kinds of detection systems, called high stopping power detectors, such as: (1) (gamma) camera with segmented thick crystal of BGO and MCP image intensifier; (2) multistep parallel plate avalanche chamber; (3) pixellized CdTe MeV photoconductor matrix. AIRIX accelerator is being

  19. Overview on CEA Contributions to the Broader Approach Projects

    International Nuclear Information System (INIS)

    Full text: France is participating to the joint Europe-Japan so-called “Broader Approach Activities” in support of ITER and DEMO activities, consisting in 3 projects: The Engineering Design and Validation of a 14 MeV neutrons irradiation facility (IFMIF/EVEDA), the building of an International Fusion Research Center (IFERC) and the ITER Satellite Tokamak Programme (STP - JT-60SA). For IFMIF/EVEDA activities, CEA is in charge of the high intensity Injector, the first part of the Superconducting RF Linac, the Cryoplant, the Accelerator Control System, the delivery of specific Beam Diagnostics and RF power tubes. This activity covers the fabrication of the systems, test of most critical components, installation and commissioning at the Japanese site. For IFERC, in 2011, CEA contracted with Bull the procurement of the “Helios” computer which reached a performance of 1.5 Petaflop/s peak, making it, with its 70000 compute cores, the largest supercomputer dedicated to a single scientific community. It is complemented by large high bandwidth storage, pre/post processing, visualization systems and services for 5 years. The work started in Sept. 2007. In Jan. 2012, first users started to work. Helios was inaugurated in March 2012. For JT-60SA tokamak, CEA is responsible for providing the Cryoplant System (CS), 9 of the 18 Toroidal Field Coils and supporting structures, the TF Coils Test Facility (CTF) and cold tests, and 5 Magnet Power Supplies (MPS). CEA is also participating to the JT-60SA Research Plan update. TF coil activities started in 2007; a manufacturing contract was placed to Alstom in July 2011. The last coil will be delivered to Japan in late 2016. Prior shipment, coils will be tested in the CTF at CEA-Saclay. The CS was optimized to smooth the transient heat loads during plasma operation. For CS and MPS contract are expected to be placed in June and Dec. 2012 respectively. This paper gives an overview of present status of these projects since 2007

  20. Radioimmunoscintigraphy of non-small cell lung cancer using Tc-99m ANTI-CEA FAB` (immuRAID CEA)

    Energy Technology Data Exchange (ETDEWEB)

    Fig, L.M. [DVA Medical Center, Ann Arbor, MI (United States); Hughes, L.; Pinsky, C.M. [Immunomedics Inc., Morris Plains, NJ (United States)] [and others

    1994-05-01

    We conducted a Phase II prospective multicenter clinical trial to investigate the safety and efficacy of Tc-99M anti-CEA Fab`(ImmuRAID-CEA) in patients with non-small cell lung cancer. Fifty four patients (42 male; 12 female; age range 40-93 yr) had primary operable (19), primary inoperable (7), occult (9), metastatic (15) or recurrent (4) disease. Patients were injected with 1 mg ImmuRAID-CEA radiolabeled with 20-30 mCi Tc-99m pertechnetate and imaged at 4-8 and 18-24 hr by planar and SPECT techniques; ten were imaged at 15-18 hr only. Despite prominent blood pool activity at 4-8 hr. overall imaging statistics on a per patient basis revealed a sensitivity of tumor detection of 90%, accuracy 85% and positive predictive value 94%. The later images had lower count rates but target to background ratios were improved. On a per lesion basis, sensitivity in the chest was 69% and in liver 88%. Twenty four patients had additional foci of antibody uptake at sites previously believed to be uninvolved; cancer was confirmed in 11, not confirmed in 2 and currently indeterminate in 11. Furthermore, a negative scan confirmed equivocal radiographic studies as true negative in 5 patients. With respect to safety, there were no adverse clinical reactions and four minor, transient changes in hematologic and/or biochemical parameters. Human anti-mouse antibody (HAMA) determinations on 27 patients using ImmuSTRIP{reg_sign} HAMA Fragment assay were negative at 4-6 weeks or 3 months post-infusion. We conclude that imaging with ImmuRAID-CEA is safe and potentially useful for the evaluation and staging of lung cancer patients.

  1. Tumor-specific accumulation of 125I-labeled mouse-human chimeric anti-CEA antibody in a xenografted human cancer model demonstrated by whole-body autoradiography and immunostaining

    International Nuclear Information System (INIS)

    Whole-body autoradiography (WBAR) was used to study the biodistribution of 125I-labeled mouse-human chimeric antibody (Ch F11-39) to carcinoembryonic antigen (CEA) in athymic nude mice bearing the CEA-producing MKN-45 human gastric carcinoma xenografts. Significantly high uptake of 125I-Ch F11-39 in the tumors obtained by tissue-counting technique was confirmed by WBAR of mice of 12, 24, 48, and 96 h postinjection of 125I-Ch F11-39. When compared with histochemical or immunohistochemical staining results of the tumor tissue sections, imaging profiles of 125I-Ch F11-39 obtained by WBARs were topographically correlated with histopathological findings of tissues and immunohistochemical localization of CEA in the tumor tissues, indicating that the accumulation of 125I-Ch F11-39 at the tumor site is based on its specificity for CEA. These results demonstrate that this chimeric antibody may serve as a potential useful diagnostic and/or therapeutic reagent for human CEA-producing cancers

  2. Poly(o-phenylenediamine) nanosphere-conjugated capture antibody immobilized on a glassy carbon electrode for electrochemical immunoassay of carcinoembryonic antigen

    International Nuclear Information System (INIS)

    We report on a new electrochemical immunosensor for the carcinoembryonic antigen (CEA; a model analyte). First, poly(o-phenylenediamine) nanospheres (PPDNSs) were synthesized by using a wet-chemistry method. The nanospheres were utilized as the support for immobilizing horseradish peroxidase-labeled polyclonal rabbit anti-human CEA antibody (HRP-anti-CEA) on a pretreated glassy carbon electrode (GCE) using glutaraldehyde as a crosslinker. In the presence of target CEA, an antigen-antibody immunocomplex formed on the electrode. This results in a partial inhibition of the active center of HRP and decreases the activity of HRP in terms of H2O2 reduction. The performance and factors influencing the performance of the immunoelectrode were studied. Under optimal conditions, the reduction current obtained from the anti-CEA-conjugated HRP (best at a working voltage of −265 mV vs. Ag/AgCl) is proportional to the CEA concentration in the 0.01 to 60 ng mL−1 range, with a detection limit of 3.2 pg mL−1. Non-specific adsorption was not observed. Relative standard deviations for intra-assay and inter-assay are <8.3 % and <9.7 %, respectively. The method was applied to the analysis of nine human serum samples, and a good relationship was found between the electrochemical immunoassay and the commercialized ELISA kit for human CEA. (author)

  3. Optimizing patient selection for dose escalation techniques using the prostate-specific antigen level, biopsy gleason score, and clinical T-stage

    International Nuclear Information System (INIS)

    Purpose: Ideal candidates for 3D dose escalation conformal radiation or external beam + implant therapy are identified on the basis of the prostate-specific antigen (PSA) level, biopsy Gleason score, and the 1992 American Joint Commission Cancer (AJCC) clinical T-stage. Methods and Materials: The pathologic findings of 1742 men with clinical stage T1c,2 prostate cancer managed with a radical prostatectomy (RP) between 1990 and 1998 were subjected to a logistic regression multivariable analysis. The endpoints examined included pathologic organ-confined (OC), specimen-confined (SC), and margin (M) or seminal vesicle (SV) positive disease. SC disease was defined as extracapsular extension (ECE) with a negative surgical margin. The clinical factors tested included PSA level, biopsy Gleason score, and the 1992 AJCC clinical T-stage. PSA failure-free (bNED) survival was calculated according to the method of Kaplan and Meier. Results: Significant negative predictors of pathologic OC-disease or positive predictors of M+ or SV+ disease included a PSA > 10 ng/ml (p + or SV+ disease respectively. Conclusions: Patients most likely to derive a survival benefit from the improved local control possible using dose escalation techniques were those who had both a low risk of having occult micrometastatic disease (+ or SV+) and a reasonable likelihood of remaining disease-free after RP (>50% 5-year bNED). These patients included those having T1c, 2a, PSA > 10-15 ng/ml, and biopsy Gleason ≤6 or T1c, 2a, 2b, PSA ≤ 10 ng/ml, and biopsy Gleason ≤ 7 prostate cancer

  4. A nomogram based on age,prostate-specific antigen level,prostate volume and digital rectal examination for predicting risk of prostate cancer

    Institute of Scientific and Technical Information of China (English)

    Ping Tang; Hui Chen; Matthew Uhlman; Yu-Rong Lin; Xiang-Rong Deng; Bin Wang; Wen-Jun Yang; Ke-Ji Xie

    2013-01-01

    Nomograms for predicting the risk of prostate cancer developed using other populations may introduce sizable bias when applied to a Chinese cohort.In the present study,we sought to develop a nomogram for predicting the probability of a positive initial prostate biopsy in a Chinese population.A total of 535 Chinese men who underwent a prostatic biopsy for the detection of prostate cancer in the past decade with complete biopsy data were included.Stepwise logistic regression was used to determine the independent predictors of a positive initial biopsy.Age,prostate-specific antigen (PSA),prostate volume (PV),digital rectal examination (DRE) status,% free PSA and transrectal ultrasound (TRUS) findings were included in the analysis.A nomogram model was developed that was based on these independent predictors to calculate the probability of a positive initial prostate biopsy.A receiver-operating characteristic curve was used to assess the accuracy of using the nomogram and PSA levels alone for predicting positive prostate biopsy.The rate for positive initial prostate biopsy was 41.7% (223/535).The independent variables used to predict a positive initial prostate biopsy were age,PSA,PV and DRE status.The areas under the receiver-operating characteristic curve for a positive initial prostate biopsy for PSA alone and the nomogram were 79.7% and 84.8%,respectively.Our results indicate that the risk of a positive initial prostate biopsy can be predicted to a satisfactory level in a Chinese population using our nomogram.The nomogram can be used to identify and Counsel patients who should consider a prostate biopsy,ultimately enhancing accuracy in diagnosing prostate cancer.

  5. The Practicality of Targeted Prostate Biopsy Procedures on the Dominant Side of the Tumor Determined by Magnetic Resonance Imaging in Elderly Patients with High Serum Levels of Prostate-Specific Antigen

    OpenAIRE

    Huh, Jung Sik; Kim, Bong Soo; Kim, Young Joo; Kim, Sung Dae; Park, Kyung Kgi

    2015-01-01

    Purpose To examine the possibility of reducing the number of cores per prostate biopsy in elderly patients with high levels of prostate-specific antigen (PSA) without significantly lowering the detection rate of prostate cancer. Materials and Methods Two hundreds sixteen men with PSA levels >20 ng/mL who underwent prostate biopsies from May 2009 to April 2013 were retrospectively reviewed. With the help of magnetic resonance imaging (MRI), the laterality of the dominant tumor burden in patien...

  6. Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA

    Science.gov (United States)

    Tian, Lihui; Liu, Li; Li, Yueyuan; Wei, Qin; Cao, Wei

    2016-01-01

    A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of carcino-embryonic antigen (CEA). This immunosensor was developed by using the trimetallic NiAuPt nanoparticles on graphene nanosheets (NGs) nanosheets (NiAuPt-NGs) as excellent labels and β-cyclodextrin functionalized reduced graphene oxide nanosheets (CD-NGs) as the platform. The CD-NGs with high specific surface area good biocompatibility and the ideal dispersibility was used to capture the primary antibodies (Ab1) efficiently. The trimetallic NiAuPt-NGs nanocomposites were used as the labels for signal amplification, showing better electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2), which is much better than that the monometallic Pt-NGs, bimetallic NiPt-NGs and AuPt-NGs due to the synergetic effect presented in NiAuPt-NGs. The NiAuPt-NGs nanocomposites consist of tightly coupled nanostructures of Au, Ni and Pt, which have neither an alloy nor a core-shell structure. Under the optimal conditions, a linear range from 0.001–100 ng/mL and a low detection limit of 0.27 pg/mL were obtained for CEA. The proposed electrochemical sandwich-type immunosensor may have a promising application in bioassay and it enriches the electrochemical immunoassays. PMID:27488806

  7. Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA.

    Science.gov (United States)

    Tian, Lihui; Liu, Li; Li, Yueyuan; Wei, Qin; Cao, Wei

    2016-01-01

    A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of carcino-embryonic antigen (CEA). This immunosensor was developed by using the trimetallic NiAuPt nanoparticles on graphene nanosheets (NGs) nanosheets (NiAuPt-NGs) as excellent labels and β-cyclodextrin functionalized reduced graphene oxide nanosheets (CD-NGs) as the platform. The CD-NGs with high specific surface area good biocompatibility and the ideal dispersibility was used to capture the primary antibodies (Ab1) efficiently. The trimetallic NiAuPt-NGs nanocomposites were used as the labels for signal amplification, showing better electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2), which is much better than that the monometallic Pt-NGs, bimetallic NiPt-NGs and AuPt-NGs due to the synergetic effect presented in NiAuPt-NGs. The NiAuPt-NGs nanocomposites consist of tightly coupled nanostructures of Au, Ni and Pt, which have neither an alloy nor a core-shell structure. Under the optimal conditions, a linear range from 0.001-100 ng/mL and a low detection limit of 0.27 pg/mL were obtained for CEA. The proposed electrochemical sandwich-type immunosensor may have a promising application in bioassay and it enriches the electrochemical immunoassays. PMID:27488806

  8. Incineration as a radioactive waste volume reduction process for CEA nuclear centers

    International Nuclear Information System (INIS)

    Incineration processes represent a promising solution for waste volume reduction, and will be increasingly used in the future. The features and performance specifications of low-level waste incinerators with capacities ranging from 10 to 20 kg-h-1 at the Fontenay-aux-Roses, Grenoble and Cadarache nuclear centers in France are briefly reviewed. More extensive knowledge of low-level wastes produced in facilities operated by the Commissariat a l'Energie Atomique (CEA) has allowed us to assess the volume reduction obtained by processing combustible waste in existing incinerators. Research and development work is in progress to improve management procedures for higher-level waste and to build facilities capable of incinerating α - contaminated waste. (authors). 6 refs., 5 figs., 1 tab

  9. CEA program on future generation light water modular reactors and gas cooled reactors

    International Nuclear Information System (INIS)

    The CEA programme on 'Future Generation Reactors and Fuel Cycles' aims at studying and developing the mean and long term most promising options for nuclear reactors, fuels and reprocessing. These options should contribute to make the nuclear energy a major source of the sustainable development. The program also aims at maintaining at the highest level of competency the technologies with which the CEA will be able to bring to national achievements or international projects in the next decades, projects whose specifications and calendar are today unknown. These studies on the 'Future Generation Reactors and Fuel Cycles' constitute a field privileged for international collaboration. The corresponding researches are structured in four main axes: Innovations for LWR; Systems of 4th generation; Sodium-cooled reactors; Systems which are the object for survey or exploratory studies. Studies on future nuclear gas technologies are mainly covered by the 4th generation programme (Gen IV). Within this context, the goals pursued, in particular the minimization of the production of long lived waste and the saving of resources (i.e.: the optimised utilisation of fissile and fertile nuclear fuels), could justify an evolution towards hard neutron spectra and high temperatures, to cover applications other than the electricity production, e.g.: hydrogen production, desalination, cogeneration. The main R and D axis for these long-term objectives currently the area of Gas Cooled Reactors (GCR). The corresponding program is structured through eight R and D projects details of which are presented within the paper. (author)

  10. Significance of the fuel cycle aspects in CEA studies on future nuclear systems

    International Nuclear Information System (INIS)

    Nuclear energy has unique assets to meet the requirements for a sustainable development in terms of economic competitiveness, environmental friendliness and natural resources saving. Future nuclear system studies conducted by the CEA aim at investigating and developing promising technologies for the medium and the long term for reactors, fuels and the fuel cycle to make nuclear power eligible as one of the major energy sources of the sustainable development. It also aims at maintaining at the best possible level the expertise and the technologies that the CEA will be able to bring to future national and international projects likely to meet market needs in the next decades, which are still uncertain both in terms of performances and time scale. Progress for future nuclear systems is principally sought in the following areas: reinforced economic competitiveness against other available electricity generation means, with a special emphasis put on reducing the investment cost; enhanced safety, especially through an increased resistance to core damages in case of severe accident, and whenever possible by dedicated strategies to exclude core melting; cleanliness through minimising the production of long lived radioactive waste; resource saving through an optimum utilisation of the available resources of fissile and fertile materials; enhanced resistance to proliferation risks; potentialities for other applications than electricity production. (author)

  11. Carcinoma-associated antigens

    International Nuclear Information System (INIS)

    This invention relates to novel antigens associated with breast carcinoma, anti-sera specific to said antigens, 125I-labeled forms of said antigens and methods of detecting said antigens in serum or plasma. The invention also relates to a diagnostic kit containing standardised antigens or antisera or marked forms thereof for the detection of said antigens in human blood, serum or plasma. (author)

  12. Producing energy without greenhouse effect gases: the CEA action

    International Nuclear Information System (INIS)

    Major actor in the domain of new energy technologies, the CEA manages the french research on the hydrogen and the fuel cells. It is also implied with INES (National Institute for the Solar Energy) in the photovoltaic and thermal solar. With the IFP (French Petroleum Institute), it manages research on biofuels. Of course the thermonuclear fusion, for the development of the energy of the future, is in its research program too. This information document presents the possibilities of these energies and the associated research programs. (A.L.B.)

  13. Sertula florae colombiae – xv: nuevas melastomatáceas

    OpenAIRE

    Uribe Uribe, Lorenzo

    2012-01-01

    El notable aumento de las colecciones del Herbario Nacional Colombiano muestran la riqueza de la flora melastomatácea de Colombia. Uno de los géneros más ricos y más bien caracterizados es Blakea. En este artículo describo dos nuevas especies muy interesantes. Dejo consignada mi gratitud para elnotable dibujante, don Silvio Fernández Valencia, a quien se deben las magníficas ilustraciones y muchos datos y mediciones de órganos pequeñísimos, que facilitaron  las descripciones exactas de las pl...

  14. The 1988 CEA progress report on laser research programs

    International Nuclear Information System (INIS)

    The 1988 CEA progress report, concerning laser investigations, is presented. The aim of the research programs on laser/matter interactions is to contribute to the military application works on this field, as well as to the development of the inertial confinement fusion physics. The activities related to the installation and starting of Phebus laser system are described. The development of soft x-ray instrumentation are included. In the field of numerical simulations, the improvement of FCI 1 and 2 codes are extended for increasing the field of the physical phenomena taken into account and getting their use more flexible to the experiment analysis

  15. Evaluation of the nuclear installations safety of the CEA in 1998

    International Nuclear Information System (INIS)

    Michel Laverie, Director of the nuclear safety and quality at the Cea, took stoke of the CEA nuclear installations in 1998. After a recall of the nuclear safety policy and organization, the author presents the risks factors bound to the CEA activities as the dismantling, the wastes and the human factors. A last part is devoted to the list of the accidents occurred during 1998 in the nuclear installations. Tables and statistics illustrate this analysis. (A.L.B.)

  16. CEA technical-economic activity report - Year 2014

    International Nuclear Information System (INIS)

    This report presents an assessment of activities undertaken by the CEA in the field of energy technique and economy during 2014. Technical-economic studies aims at contributing to national orientations on energy, at R and D program orientations, at highlighting and strengthening synergies between nuclear technologies and new energy technologies, and at strengthening the credibility of these technologies. After a presentation of the organisation of technique and economy within the CEA, of the involved bodies and departments, of the addressed themes (photovoltaic, wind, biomass and biofuels, hydrogen-based systems, mobility, electric systems and grids), the report presents studies performed in relationship with the national energy strategy and with energy scenarios, studies performed in the nuclear field (on fourth-generation reactors, on the front-end and back-end of the fuel cycle), studies performed in the field of new energy technologies and climate (hydrogen and storage, biofuels, climate, mobility, solar, electric systems). The next part addresses academic aspects, methodological studies and modelling studies. Contributions and participations to conferences, and publications are indicated

  17. 3rd CEAS Specialist Conference on Guidance, Navigation and Control

    CERN Document Server

    Drouin, Antoine; Roos, Clément

    2015-01-01

    The two first CEAS (Council of European Aerospace Societies) Specialist Conferences on Guidance, Navigation and Control (CEAS EuroGNC) were held in Munich, Germany in 2011 and in Delft, The Netherlands in 2013. ONERA The French Aerospace Lab, ISAE (Institut Supérieur de l’Aéronautique et de l’Espace) and ENAC (Ecole Nationale de l’Aviation Civile) accepted the challenge of jointly organizing the 3rd edition. The conference aims at promoting new advances in aerospace GNC theory and technologies for enhancing safety, survivability, efficiency, performance, autonomy and intelligence of aerospace systems. It represents a unique forum for communication and information exchange between specialists in the fields of GNC systems design and operation, including air traffic management. This book contains the forty best papers and gives an interesting snapshot of the latest advances over the following topics: l  Control theory, analysis, and design l  Novel navigation, estimation, and tracking methods l  Aircr...

  18. Development of a PMMA Electrochemical Microfluidic Device for Carcinoembryonic Antigen Detection

    Science.gov (United States)

    Van Anh, Nguyen; Van Trung, Hoang; Tien, Bui Quang; Binh, Nguyen Hai; Ha, Cao Hong; Le Huy, Nguyen; Loc, Nguyen Thai; Thu, Vu Thi; Lam, Tran Dai

    2016-05-01

    In this study, a poly(methyl methacrylate) (PMMA) microfluidic device fabricated by an inexpensive CO2 laser etching system was developed for detection of carcino-embryonic antigens (CEA). The device was capable of working in continuous mode and was designed with the aid of numerical simulation. The detection of target CEA was based on immuno-assay via magnetic particles and electrochemical sensing. The as-prepared microfluidic can be used to detect CEA at the relatively low concentration of 150 pg mL-1. The device could be reused many times, since the capture and removal of magnetic particles in the assay could be manipulated by an external magnetic field. The proposed approach appears to be suitable for high-throughput and automated analysis of large biomolecules such as tumor markers and pathogens.

  19. Isolation of Fasciola hepatica tegument antigens.

    OpenAIRE

    Hillyer, G. V.

    1980-01-01

    Fasciola hepatica tegument antigens were isolated from intact worms in the cold by using Nonidet P-40. Proof of the tegumental nature of the antigens was shown by the peroxidase-antiperoxidase immunocytochemical technique at the light microscope level. The potential of F. hepatica tegument antigens for the immunodiagnosis of rabbit and human fascioliasis was shown by Ouchterlony immunodiffusion, although cross-reactivity was evident in one of six serum samples from patients infected with Schi...

  20. Association of Neisseria gonorrhoeae Opa(CEA with dendritic cells suppresses their ability to elicit an HIV-1-specific T cell memory response.

    Directory of Open Access Journals (Sweden)

    Qigui Yu

    Full Text Available Infection with Neisseria gonorrhoeae (N. gonorrhoeae can trigger an intense local inflammatory response at the site of infection, yet there is little specific immune response or development of immune memory. Gonococcal surface epitopes are known to undergo antigenic variation; however, this is unlikely to explain the weak immune response to infection since individuals can be re-infected by the same serotype. Previous studies have demonstrated that the colony opacity-associated (Opa proteins on the N. gonorrhoeae surface can bind human carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1 on CD4⁺ T cells to suppress T cell activation and proliferation. Interesting in this regard, N. gonorrhoeae infection is associated with impaired HIV-1 (human immunodeficiency virus type 1-specific cytotoxic T-lymphocyte (CTL responses and with transient increases in plasma viremia in HIV-1-infected patients, suggesting that N. gonorrhoeae may also subvert immune responses to co-pathogens. Since dendritic cells (DCs are professional antigen presenting cells (APCs that play a key role in the induction of an adaptive immune response, we investigated the effects of N. gonorrhoeae Opa proteins on human DC activation and function. While morphological changes reminiscent of DC maturation were evident upon N. gonorrhoeae infection, we observed a marked downregulation of DC maturation marker CD83 when the gonococci expressing CEACAM1-specific Opa(CEA, but not other Opa variants. Consistent with a gonococcal-induced defect in maturation, Opa(CEA binding to CEACAM1 reduced the DCs' capacity to stimulate an allogeneic T cell proliferative response. Moreover, Opa(CEA-expressing N. gonorrhoeae showed the potential to impair DC-dependent development of specific adaptive immunity, since infection with Opa(CEA-positive gonococci suppressed the ability of DCs to stimulate HIV-1-specific memory CTL responses. These results reveal a novel mechanism to explain

  1. Association of Neisseria gonorrhoeae Opa(CEA) with dendritic cells suppresses their ability to elicit an HIV-1-specific T cell memory response.

    Science.gov (United States)

    Yu, Qigui; Chow, Edith M C; McCaw, Shannon E; Hu, Ningjie; Byrd, Daniel; Amet, Tohti; Hu, Sishun; Ostrowski, Mario A; Gray-Owen, Scott D

    2013-01-01

    Infection with Neisseria gonorrhoeae (N. gonorrhoeae) can trigger an intense local inflammatory response at the site of infection, yet there is little specific immune response or development of immune memory. Gonococcal surface epitopes are known to undergo antigenic variation; however, this is unlikely to explain the weak immune response to infection since individuals can be re-infected by the same serotype. Previous studies have demonstrated that the colony opacity-associated (Opa) proteins on the N. gonorrhoeae surface can bind human carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) on CD4⁺ T cells to suppress T cell activation and proliferation. Interesting in this regard, N. gonorrhoeae infection is associated with impaired HIV-1 (human immunodeficiency virus type 1)-specific cytotoxic T-lymphocyte (CTL) responses and with transient increases in plasma viremia in HIV-1-infected patients, suggesting that N. gonorrhoeae may also subvert immune responses to co-pathogens. Since dendritic cells (DCs) are professional antigen presenting cells (APCs) that play a key role in the induction of an adaptive immune response, we investigated the effects of N. gonorrhoeae Opa proteins on human DC activation and function. While morphological changes reminiscent of DC maturation were evident upon N. gonorrhoeae infection, we observed a marked downregulation of DC maturation marker CD83 when the gonococci expressing CEACAM1-specific Opa(CEA), but not other Opa variants. Consistent with a gonococcal-induced defect in maturation, Opa(CEA) binding to CEACAM1 reduced the DCs' capacity to stimulate an allogeneic T cell proliferative response. Moreover, Opa(CEA)-expressing N. gonorrhoeae showed the potential to impair DC-dependent development of specific adaptive immunity, since infection with Opa(CEA)-positive gonococci suppressed the ability of DCs to stimulate HIV-1-specific memory CTL responses. These results reveal a novel mechanism to explain why

  2. Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant

    Science.gov (United States)

    Vasquez, Abel E; Manzo, Ricardo A; Soto, Daniel A; Barrientos, Magaly J; Maldonado, Aurora E; Mosqueira, Macarena; Avila, Anastasia; Touma, Jorge; Bruce, Elsa; Harris, Paul R; Venegas, Alejandro

    2015-01-01

    The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against H. pylori protein increased. Splenocytes from PorA-immunized mice were stimulated with PorA, and an increase in the secretion of IL-4 was observed compared with that of IFN-γ. Moreover, in an immunoglobulin sub-typing analysis, a substantially higher IgG1 level was found compared with IgG2a levels, suggesting a Th2-type immune response. This study revealed a peculiar behavior of the purified recombinant PorA protein per se in the absence of AbISCO as an adjuvant. Therefore, the resistance of PorA to proteolytic enzymes, such as those in the gastrointestinal tract, was analyzed, because this is an important feature for an oral protein adjuvant. Finally, we found that PorA fused to the H. pylori HpaA antigen, when expressed in Lactococcus lactis and administered orally, could enhance the antibody response against the HpaA antigen approximately 3 fold. These observations strongly suggest that PorA behaves as an effective oral adjuvant. PMID:25750999

  3. Scientific evaluation at the French Atomic Energy Commission (CEA). 2005-2006 annual report; L'evaluation scientifique au CEA. Rapport annuel 2005-2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This report aims at presenting the scientific evaluation activities carried out at the CEA during the years 2005-2006. The evaluation system is described in detail. It comprises two aspects: the evaluation of the scientific policy implemented by the scientific Council and by the visiting committee of the CEA, and the evaluation of the laboratories, performed by a pool of 36 scientific councils. The evaluation by external and independent parties is the key point of this system. This document makes a status of the evaluations performed in 2005 and 2006. It presents a synthesis of the conclusions of the evaluation authorities. The actions implemented by the CEA to take into consideration the recommendations are also reported with the improvements noticed. The two topics examined by the scientific Committee and by the visiting committee were dealing with the energy domain, which is a strategic issue for the CEA. The examination of the researches on future nuclear reactors and on new energy technologies have shown the major role played by the CEA in the recent advances in these domains. About 95% of the laboratories activity was examined during the 2002-2005 period. The richness of the remarks and recommendations made by the scientific councils should allow the CEA to improve the quality and relevance of its research works. The start-up of the 2006-2009 evaluation cycle has been the occasion to modify the evaluation of some research domains in order to take into account the evolution of programs. The evaluation system of the CEA is highly consistent with the AERES principles. Its implementation, adapted to each type of activity (fundamental research, applied research, technological developments) allows the CEA to follow up a permanent improvement approach. (J.S.)

  4. RHAMM (CD168) Is Overexpressed at the Protein Level and May Constitute an Immunogenic Antigen in Advanced Prostate Cancer Disease1

    OpenAIRE

    Kilian M. Gust; Hofer, Matthias D; Sven R. Perner; Kim, Robert; Arul M. Chinnaiyan; Varambally, Sooryanarayana; Moller, Peter; Rinnab, Ludwig; Rubin, Mark A; Greiner, Jochen; Schmitt, Michael; Kuefer, Rainer; Ringhoffer, Mark

    2009-01-01

    Localized prostate cancer (CaP) can be cured using several strategies. However, the need to identify active substances in advanced tumor stages is tremendous, as the outcome in such cases is still disappointing. One approach is to deliver human tumor antigen-targeted therapy, which is recognized by T cells or antibodies. We used data mining of the Cancer Immunome Database (CID), which comprises potential immunologic targets identified by serological screening of expression libraries. Candidat...

  5. Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen.

    OpenAIRE

    Adnan Ahmad; Gurramkonda Chandrasekhar; Lünsdorf Heinrich; Khanna Navin; Rinas Ursula

    2011-01-01

    Abstract Background A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, the Hepatitis B surface antigen (HBsAg). Thus, the Hepatitis B vaccine is generated through the utilization of recombinant DNA technology, preferentially by using yeast-based expression systems. However, the polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs), to elicit the required protective immune ...

  6. Acute Lymphoid Leukemia Cells with Greater Stem Cell Antigen-1 (Ly6a/Sca-1) Expression Exhibit Higher Levels of Metalloproteinase Activity and Are More Aggressive In Vivo

    OpenAIRE

    Yu-Chiao Hsu; Kurt Mildenstein; Kordell Hunter; Olena Tkachenko; Mullen, Craig A.

    2014-01-01

    Stem cell antigen-1 (Ly6a/Sca-1) is a gene that is expressed in activated lymphocytes, hematopoietic stem cells and stem cells of a variety of tissues in mice. Despite decades of study its functions remain poorly defined. These studies explored the impact of expression of this stem cell associated gene in acute lymphoid leukemia. Higher levels of Ly6a/Sca-1 expression led to more aggressive leukemia growth in vivo and earlier death of hosts. Leukemias expressing higher levels of Ly6a/Sca-1 ex...

  7. Assessment 2000 and regulation and method. Releases control and environmental survey of the CEA Centers

    International Nuclear Information System (INIS)

    The environment quality around the CEA centers is a major interest of its safety policy. These documents contribute to the public information on the radioactive liquid and gaseous releases of the CEA, according to the ministry authorization. The radioactivity monitoring activity, and the survey methods are also presented. Data analysis from 1996 to 2000, allows to follow the evolution. (A.L.B.)

  8. An ultrasensitive chemiluminescence biosensor for carcinoembryonic antigen based on autocatalytic enlargement of immunogold nanoprobes.

    Science.gov (United States)

    Hao, Minjia; Ma, Zhanfang

    2012-01-01

    A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl(4)- to Au0 on their surface in the presence of NH(2)OH·HCl. AuCl(4)-, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg∙mL-1 to 1,000 ng∙mL-1, with a detection limit of 20 pg∙mL-1. PMID:23443399

  9. An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes

    Directory of Open Access Journals (Sweden)

    Minjia Hao

    2012-12-01

    Full Text Available A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA detection based on signal amplification with gold nanoparticles (NPs is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl4− to Au0 on their surface in the presence of NH2OH·HCl. AuCl4−, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg∙mL−1 to 1,000 ng∙mL−1, with a detection limit of 20 pg∙mL−1.

  10. General rules for radiation protection within the CEA

    International Nuclear Information System (INIS)

    This report first describes the organisation, scope of application and principles of radiation protection within the CEA. The second part proposes an overview of rules applicable to workers exposed to ionizing radiation. These rules concern the personnel classification, exposure limits, individual control, and training of workers in radiation protection. The third part addresses the technical rules for premise laying-out: protection zoning, indicators aimed at controlling the compliance of premise classification, radiological survey and control of premise classification. The fourth part addresses the modalities of access, stay and operation in regulated zone. The next part indicates and comments arrangements specific to ionizing radiation sources and to ionizing radiation emitting equipment (authorization and possession modalities, training, certification, controls, operation zoning, etc.), specific orders (radiological control of wastes, hardware, tools and equipment management), rules related to abnormal occupational situations (alarms, contamination event, radiological events), and the different studies and analysis of radiation protection implemented during the different phases of an installation lifetime

  11. Report on transparency and nuclear safety 2014 - Cadarache CEA centre

    International Nuclear Information System (INIS)

    This document proposes, first, a presentation of the Cadarache CEA centre, of its activities and installations, gives a rather detailed overview of measures related to safety and to radiation protection within these activities and installations. Then it reports significant events related to safety and to radiation protection which occurred in 2014 and have been declared to the ASN. Next, it discusses the results of release measurements (liquid and gaseous effluents, radiological assessment, and chemical assessment for various installations) and the control of the chemical and radiological impact of these gaseous and liquid effluents on the environment. Finally, it addresses the issue of radioactive wastes which are stored in the different nuclear base installations of the Centre, indicates the different measures aimed at limiting the volume of these warehoused wastes and addresses their impact on health and on the environment. Nature and quantities of warehoused wastes are specified. Remarks and recommendations of the CHSCT are given

  12. Cryogenic turbulence test facilities at CEA/SBT

    Science.gov (United States)

    Rousset, B.; Baudet, C.; Bon Mardion, M.; Bourgoin, M.; Braslau, A.; Daviaud, F.; Diribarne, P.; Dubrulle, B.; Gagne, Y.; Gallet, B.; Gibert, M.; Girard, A.; Lehner, T.; Moukharski, I.; Sy, F.

    2015-12-01

    Recently, CEA Grenoble SBT has designed, built and tested three liquid helium facilities dedicated to turbulence studies. All these experiments can operate either in HeI or HeII within the same campaign. The three facilities utilize moving parts inside liquid helium. The SHREK experiment is a von Kármán swirling flow between 0.72 m diameter counterrotating disks equipped with blades. The HeJet facility is used to produce a liquid helium free jet inside a 0.200 m I.D., 0.47 m length stainless steel cylindrical testing chamber. The OGRES experiment consists of an optical cryostat equipped with a particle injection device and an oscillating grid. We detail specific techniques employed to accommodate these stringent specifications. Solutions for operating these facilities without bubbles nor boiling/cavitation are described. Control parameters as well as Reynolds number and temperature ranges are given.

  13. The stationary storage of energy. Available technologies and CEA researches

    International Nuclear Information System (INIS)

    After a discussion of the main challenges related to the stationary storage of energy, this publication proposes an overview of the different available technologies: plant for transfer of energy by pumping, compressed air, energy flywheels, hydrogen, lithium-ion battery, redox-flow battery, thermal storage by sensitive heat, thermal-chemical storage coupled to a thermal solar system, thermal storage by phase change, superconductive inductance storage, super-capacitors. It discusses the criteria of choice of storage technology, either for electric energy storage or for heat storage. It proposes an overview of researches performed within the CEA on storage systems: electrochemical, thermal, and hydrogen-based storages. The final chapter addresses current fundamental researches on storage in the field of lithium-ion batteries, hydrogen as a fuel, and thermoelectricity

  14. Recent advances in indirect drive ICF target physics at CEA

    International Nuclear Information System (INIS)

    The objective of Target Physics Program at CEA is the achievement of ignition on the LMJ, a glass laser facility of 1.8 MJ which will be completed by 2008. It is composed of theoretical work, experimental work and numerical simulations. An important part of experimental studies is made in collaboration with U.S. DOE Laboratories: Lawrence Livermore National Laboratory, Los Alamos National Laboratory and the Laboratory for Laser Energetics at the University of Rochester. Experiments were performed on Phebus, NOVA (LLNL) and OMEGA (LLE) ; they included diagnostics developments. Recent efforts have been focused on Laser Plasma Interaction, hohlraum energetics, symmetry, ablator physics and hydrodynamic instabilities. Ongoing work prepare the first experiments on the LIL which is a prototype facility of the LMJ (8 of its 240 beams). They will be performed by 2002. Recent progress in ICF target physics allows us to precise laser specifications to achieve ignition with reasonable margin. (author)

  15. CEA contribution to the ITER ICRH antenna design

    International Nuclear Information System (INIS)

    The ITER ion-cyclotron range of frequency (ICRH) heating system is required to couple 20 MW of power in the frequency range 40–55 MHz for a large range of scenarios with Edge Localized Modes. To mitigate the associated risks, it is foreseen to design and install on ITER two port-plug antennas for a total of 20 MW coupled power on long pulse operation [1]. The CEA activity to this antenna design within the CYCLE consortium was focused on the Faraday screen design (Fig. 1) and associated radio frequency (RF) sheath modelling, the reflectometers design for edge density measurement in the antenna vicinity and a contribution to the remote handling/tooling of the antenna. This paper is an overview of each item and proposes some R and D activities on key components

  16. Planta de lavado para arena silícea

    OpenAIRE

    Rojo García, Cristina

    2008-01-01

    El proyecto fin de carrera trata sobre la instalación de una planta móvil de lavado para arena silícea. Este recurso está presente en el Monte Público nº 90 denominado “RAJICA DE EN MEDIO” en el Término Municipal de Jumilla (Murcia), donde se ubica la cantera “Los Mochuelos” de la que es concesionaria y explotadora la empresa JUMILLANA DE SÍLICE, S.L, con C.I.F. nº B-25.646.720, con domicilio social en C/ Canalejas nº 83, Bajo, de Jumilla (Murcia). Por ello, el presente proyecto tien...

  17. Development of radiolabelling techniques of anti-CEA monoclonal antibody

    International Nuclear Information System (INIS)

    The purpose of this work was to label monoclonal and polyclonal antibodies with 99Tcm such as the ior-CEA-1 antibody and polyclonal IgG using a direct method, to check the radiochemical and biological behavior of labelled products, to prepare it under sterile and apyrogenic conditions as a lyophilized kit and to employ it in clinical trials. In addition, a photoactivation method was used to label polyclonal IgG with 99Tcm and to compare with the established method using mercaptoethanol (2-ME) as the reducing agent. Finally polyclonal IgG was labelled using an indirect method in which a chelator was covalently attached to the protein and the 99Tcm added as glucoheptonate complex. The properties of 99Tcm when labelled with monoclonal and polyclonal antibodies by different methods were assessed by in vitro and in vivo studies

  18. Simultaneous determination of carcinoembryonic antigen and α-fetoprotein using an ITO immunoelectrode modified with gold nanoparticles and mesoporous silica

    International Nuclear Information System (INIS)

    We report on an electrochemical immunoassay for simultaneous detection of the tumor markers carcinoembryonic antigen (CEA) and α-fetoprotein (AFP). Gold nanoparticles (AuNPs) were synthesized inside the internal pore walls of mesoporous silica (MPS). Then, Methylene blue (MB) and 6-ferrocenylhexanethiol (FeC), which act as electrochemical substrates, were incorporated into the channels via the formation of Au-S bonds. Finally, monoclonal antibodies against CEA (anti-CEA) and AFP (anti-AFP) were immobilized in the channels of MB-AuNPs-MPS and FeC-AuNPs-MPS, respectively. Suspensions of anti-CEA/MB-AuNPs-MPS and of anti-AFP/FeC-AuNPs-MPS were deposited on two different sites of the ITO electrode. When incubated with samples containing the two analytes, the formation of electrically nonconductive immunoconjugates blocks electron transfer. Simultaneous detection of CEA and AFP was accomplished by monitoring the current change before and after an immunoreaction has occurred. The AuNPs confined inside the channels are promoting the electron transport and thus improve detection limits. The immunoelectrode responds to CEA in the 0.5–50 ng mL−1 concentration range, and to AFP in concentrations between 0.5 and 100 ng mL−1. The detection limits (at an S/N of 3) are 0.1 ng mL−1 in both cases. (author)

  19. Evaluation of tumor markers carcinoembryonic antigen, cytokeratin 19 fragment and cancer-associated antigen 72-4 in neoplastic and non-neoplastic canine effusions differentiation

    OpenAIRE

    L.V Teixeira; T.A. Guerra; F.O. Conrado; S.R. Terra; D.G. Gerardi; González, F.H.D.

    2014-01-01

    The concentration of tumor markers in body fluids can be used for diagnosis and prognosis of patients. This study aimed to investigate the performance of tumor markers cytokeratin 19 fragment (CYFRA 21-1), cancer-associated antigen 72-4 (CA 72-4) and carcinoembryonic antigen (CEA) in the neoplastic and non-neoplastic canine effusions. In thirty-two neoplastic (n=16) and non-neoplastic (n=16) samples of canine thoracic or abdominal effusions, tumor markers were measured. Significant statistica...

  20. Experimental Feedback on Sodium Loop Decommissioning at the CEA

    International Nuclear Information System (INIS)

    The aim of this paper is to present experimental feedback on sodium loop dismantling techniques at the CEA (The French Atomic Energy Commission) and to offer recommendations for the decommissioning of Fast Breeder Reactor secondary sodium loops. This study is divided into several parts which correspond to the different stages of a dismantling system. It is based on acquired CEA decommissioning experience which primarily concerns the following: the decommissioning of Rapsodie (France's first Fast Breeder Reactor), the Phenix reactor secondary loop replacement, the sodium loop decommissioning carried out by the Laboratory of Sodium Technologies and Treatment, and several technical documents. This paper deals with the main results of this survey. First, a comparison of 8 pipe-cutting techniques is made, taking into account speed in cutting, reliability, dissemination, fire risk due to the presence of sodium, cutting depth, and different types of waste (empty pipes, sodium-filled pipes, tanks...). This comparison has led us to recommend the use of an alternative saw or a chain saw rather than the use of the plasma torch or grinder. Different techniques are recommended depending on if they are on-site, initial cuttings or if they are to be carried out in a specially-designed facility referred to hereafter as 'the cutting building'. After the cutting stage, the sodium waste must be processed with water to become an ultimate stable waste. Four treatment processes are compared with different standards: speed, cost, low activity adaptability and 'large sodium quantity' adaptability. Recommendations are also made for reliable storage, and for the general dismantling system organization. Last, calculations are presented concerning a complete dismantling facility prototype capable of treating large amounts of sodium. (authors)

  1. The antigenic properties of human prolactin

    International Nuclear Information System (INIS)

    The antigenic properties of human prolactin (HPr) were studied using various methods of radio-immuno assay. The homologous system, the difficulty of which resides in the preparation of the tracer, easily permits measurement of physiological levels. In this system, blood prolactin in the monkey has an antigenicity comparable with that of human prolactin, whereas growth hormone and human chorionic somatotropin have feeble or nil antigenic relationship with HPr. Human, sheep and pig prolactins have variable antigenic cross-reactions depending on the immune serum used. These antigenic cross reactions may be applied to the isolation of amniotic prolactin. Human blood prolactin has several components of different molecular weight, but antigenicity comparable with that of pituitary HPr

  2. A novel label-free microfluidic paper-based immunosensor for highly sensitive electrochemical detection of carcinoembryonic antigen.

    Science.gov (United States)

    Wang, Yang; Xu, Huiren; Luo, Jinping; Liu, Juntao; Wang, Li; Fan, Yan; Yan, Shi; Yang, Yue; Cai, Xinxia

    2016-09-15

    In this work, a highly sensitive label-free paper-based electrochemical immunosensor employing screen-printed working electrode (SPWE) for detection of carcinoembryonic antigen (CEA) was fabricated. In order to raise the detection sensitivity and immobilize anti-CEA, amino functional graphene (NH2-G)/thionine (Thi)/gold nanoparticles (AuNPs) nanocomposites were synthesized and coated on SPWE. The principle of the immunosensor determination was based on the fact that the decreased response currents of Thi were proportional to the concentrations of corresponding antigens due to the formation of antibody-antigen immunocomplex. Experimental results revealed that the immunoassay enabled the determination of standard CEA solutions with linear working ranges of 50pgmL(-1) to 500ngmL(-1), the limit of detections for CEA is 10pgmL(-1) (S/N=3) and its corresponding correlation coefficients were 0.996. Furthermore, the proposed immunosensor could be used for the determination of clinical serum samples. A large number of clinical serum samples were detected and the relative errors between measured values and reference concentrations were calculated. Results showed that this novel paper-based electrochemical immunosensor could provide a new platform for low cost, sensitive, specific, and point-of-care diagnosis in cancer detection. PMID:27132007

  3. Distinct CDR3 Conformations in T Cell Receptors Determine the Level of Cross-Reactivity for Diverse Antigens, but Not the Docking Orientation

    OpenAIRE

    Jones, Lindsay L.; Colf, Leremy A.; Stone, Jennifer D.; Garcia, K. Christopher; Kranz, David M.

    2008-01-01

    T cells are known to cross-react with diverse peptide MHC antigens through their αβ TCRs. To explore the basis of such cross-reactivity, we examined the 2C TCR that recognizes two structurally distinct ligands, SIY-Kb and alloantigen QL9-Ld. Here we characterized the crossreactivity of several high affinity 2C TCR variants that contained mutations only in the CDR3α loop. Two of the TCRs lost their ability to cross-react with the reciprocal ligand (SIY-Kb), while another TCR (m67) maintained r...

  4. Hepatitis B surface antigen quantity positively correlates with plasma levels of microRNAs differentially expressed in immunological phases of chronic hepatitis B in children

    DEFF Research Database (Denmark)

    Winther, Thilde Nordmann; Heiberg, Ida Louise; Bang-Berthelsen, Claus Heiner;

    2013-01-01

    Children with chronic hepatitis B (CHB) are at high risk of progressive liver disease. It is suggested that a newly-identified panel of 16 microRNAs is important in the pathogenesis of CHB in children. Subviral hepatitis B surface antigen (HBsAg) particles are produced in large excess over infect...... infectious virions. Interestingly, circulating HBsAg particles have been shown to carry microRNAs. A thorough characterisation of the identified microRNAs and HBsAg over time in plasma from children with CHB may provide useful information about the natural course of childhood CHB....

  5. Histocompatibility antigen test

    Science.gov (United States)

    ... more common in certain autoimmune diseases . For example, HLA-B27 antigen is found in many people (but not ... More Ankylosing spondylitis Autoimmune disorders Bone marrow transplant HLA-B27 antigen Kidney transplant Reactive arthritis Update Date 2/ ...

  6. Design and cancer-targeting potential of antibody-based molecules directed against carcinoembryonic antigen.

    OpenAIRE

    Huhalov, A.

    2004-01-01

    This thesis examines the use of protein engineering to create antibody-based molecules for cancer treatment. The targeting unit used for these molecules was the single chain Fv antibody fragment MFE-23, which is directed against the tumour-associated marker carcinoembryonic antigen (CEA). It was hypothesised that implementation of molecular design features such as humanisation, high affinity, multivalency and mannose glycosylation to accelerate systemic clearance would result in the favourabl...

  7. Development work on the SABRE code done by the CEA. Introduction of a new detailed wire-wrap model

    International Nuclear Information System (INIS)

    The versatility of the SABRE 2A code was extended by the CEA through the inclusion of a detailed treatment of the helical wire spacers, which is coherent with the sharp tridimensional analysis at the basis of the code development. This model was tested satisfactorily in comparison with experimental data from assemblies geometrically similar to CRBR and SPX1 fuel assemblies. The complex flow field inside the bundle seems well calculated, which enables to represent the mixing effects of the wire spacers at a macroscopic level

  8. Biodistribution and tumor imaging of an anti-CEA single-chain antibody-albumin fusion protein

    Energy Technology Data Exchange (ETDEWEB)

    Yazaki, Paul J. [Division of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010 (United States)], E-mail: pyazaki@coh.org; Kassa, Thewodros; Cheung, Chia-wei; Crow, Desiree M. [Division of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010 (United States); Sherman, Mark A. [Division of Information Sciences, Beckman Research Institute, City of Hope, Duarte, CA 91010 (United States); Bading, James R.; Anderson, Anne-Line J.; Colcher, David; Raubitschek, Andrew [Division of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010 (United States)

    2008-02-15

    Albumin fusion proteins have demonstrated the ability to prolong the in vivo half-life of small therapeutic proteins/peptides in the circulation and thereby potentially increase their therapeutic efficacy. To evaluate if this format can be employed for antibody-based imaging, an anticarcinoembryonic antigen (CEA) single-chain antibody(scFv)-albumin fusion protein was designed, expressed and radiolabeled for biodistribution and imaging studies in athymic mice bearing human colorectal carcinoma LS-174T xenografts. The [{sup 125}I]-T84.66 fusion protein demonstrated rapid tumor uptake of 12.3% injected dose per gram (ID/g) at 4 h that reached a plateau of 22.7% ID/g by 18 h. This was a dramatic increase in tumor uptake compared to 4.9% ID/g for the scFv alone. The radiometal [{sup 111}In]-labeled version resulted in higher tumor uptake, 37.2% ID/g at 18 h, which persisted at the tumor site with tumor: blood ratios reaching 18:1 and with normal tissues showing limited uptake. Based on these favorable imaging properties, a pilot [{sup 64}Cu]-positron emission tomography imaging study was performed with promising results. The anti-CEA T84.66 scFv-albumin fusion protein demonstrates highly specific tumor uptake that is comparable to cognate recombinant antibody fragments. The radiometal-labeled version, which shows lower normal tissue accumulation than these recombinant antibodies, provides a promising and novel platform for antibody-based imaging agents.

  9. Pharmacokinetics and Dosimetry Studies for Optimization of Pretargeted Radioimmunotherapy in CEA-Expressing Advanced Lung Cancer Patients

    Directory of Open Access Journals (Sweden)

    Caroline eBodet-Milin

    2015-11-01

    Full Text Available Objectives. A phase I pretargeted radioimmunotherapy trial (EudractCT 200800603096 was designed in patients with metastatic lung cancer expressing carcinoembryonic antigen (CEA to optimize bispecific antibody and labelled peptide doses, as well as the delay between their injections.Methods. Three cohorts of 3 patients received the anti-CEA x anti-histamine-succinyl-glycine (HSG humanized trivalent bispecific antibody (TF2 and the IMP288 bivalent HSG-peptide. Patients underwent a pre-therapeutic imaging session S1 (44 or 88 nmol/m2 of TF2 followed by 4.4 nmol/m2, 185 MBq, of 111In-labelled IMP288, and, 1-2 weeks later, a therapy session S2 (240 or 480 nmol/m2 of TF2 followed by 24 nmol/m2, 1.1 GBq/m2, 177Lu-labeled IMP288. The pretargeting delay was 24 or 48 hours. The dose schedule was defined based on pre-clinical TF2 pharmacokinetic studies, on our previous clinical data using the previous anti-CEA pretargeting system and on clinical results observed in the first patients injected using the same system in the Netherlands.Results. TF2 pharmacokinetics (PK was represented by a two-compartment model in which the central compartment volume was linearly dependent on the patient's surface area. PK were remarkably similar, with a clearance of 0.33 +/- 0.03 L/h per m2. 111In- and 177Lu-IMP288 PK were also well represented by a two-compartment model. IMP288 PK were faster (clearance 1.4 to 3.3 l/h. The central compartment volume was proportional to body surface area and IMP288clearance depended on the molar ratio of injected IMP288 to circulating TF2 at the time of IMP288 injection. Modelling of image quantification confirmed the dependence of IMP288 kinetics on circulating TF2, but tumour activity PK were variable. Organ absorbed doses were not significantly different in the 3 cohorts, but the tumour dose was significantly higher with the higher molar doses of TF2 (p < 0.002. S1 imaging predicted absorbed doses calculated in S2. Conclusion. The best

  10. Fusion technology. Annual report of the. Association Cea/EURATOM; Technologie de fusion.Rapport annuel de l`association CEA/Euratom

    Energy Technology Data Exchange (ETDEWEB)

    Magaud, P.; Le Vagueres, F.

    1996-12-31

    In 1996, the French EURATOM-CEA Association made significant contributions to the European technology programme. This work is compiled in this report as follows: the ITER CEA activities and related developments are described in the first section; blankets and material developments for DEMO, long term safety studies are summarised in the second part; the Underlying Technology activities are compiled in the third part of this report. In each section, the tasks are sorted out to respect the European presentation. For an easy reading, appendix 4 gives the list of tasks in alphabetical order with a page reference list. The CEA is in charge of the French Technology programme. Three specific organizational directions of the CEA, located on four sites (see appendix 5) are involves in this programme: Advanced Technologies Direction (DTA), for Material task; Nuclear Reactors Direction (DRN), for Blanket design, Neutronic problems, Safety tasks; Physical Sciences Direction (DSM) uses the competence of the Tore Supra team in the Magnet design and plasma Facing Component field. The CEA programme is completed by collaborations with Technicatome, COMEX-Nucleaire and Ecole Polytechnique. The breakdown of the programme by Directions is presented in figure 1. The allocation of tasks is given in appendix 2 and in appendix 3, the related publications. (author).

  11. Imaging of pharyngeal and laryngeal carcinomas with indium-111-labeled monoclonal anti-CEA antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Kairemo, K.J.; Hopsu, E.V. (Helsinki Univ. Central Hospital (Finland))

    1990-10-01

    Localization of primary tumors, metastases, or recurrences in 13 consecutive patients with histological verification of squamous cell or adenocarcinoma was made with radioimmunodetection using monoclonal radiolabeled anti-CEA antibody. All surgical specimens stained immunohistochemically, except one, were positive for CEA. Of the known 19 tumor sites 17 were visualized in antibody scans. There were two positive findings that did not prove to be positive during 12 month follow-up. The scintigram findings did not correlate with CEA serum concentrations that, with one exception, were normal in all patients.

  12. Imaging of pharyngeal and laryngeal carcinomas with indium-111-labeled monoclonal anti-CEA antibodies

    International Nuclear Information System (INIS)

    Localization of primary tumors, metastases, or recurrences in 13 consecutive patients with histological verification of squamous cell or adenocarcinoma was made with radioimmunodetection using monoclonal radiolabeled anti-CEA antibody. All surgical specimens stained immunohistochemically, except one, were positive for CEA. Of the known 19 tumor sites 17 were visualized in antibody scans. There were two positive findings that did not prove to be positive during 12 month follow-up. The scintigram findings did not correlate with CEA serum concentrations that, with one exception, were normal in all patients

  13. The value of combined detection of serum SCCA,CYFRA21-1 and CEA in diagnosis of lung cancer%血清SCCA、CYFRA21-1、CEA联合检测在肺癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    王小芳; 罗玲

    2013-01-01

    目的 评价血清鳞状细胞癌抗原(SCCA)、细胞角蛋白片段21-1(CYFRA21-1)、癌胚抗原(CEA)联合检测在肺癌诊断中的价值.方法 选择重庆市肿瘤研究所2008年1~4月住院患者共63例,收集所有患者血清检测SCCA、CYFRA21-1及CEA.其中肺癌44例,肺部良性病变者19例,肺癌患者中小细胞肺癌5例、鳞癌17例、腺癌15例,未定型肺癌7例,所有肺癌患者均为病理确诊.结果 肺癌组SCCA、CYFRA21-1、CEA水平均高于肺部良性病变组(P<0.01),在不同病理类型中,3种指标升高的程度不等,SCCA 及CYFRA21-1水平在鳞癌组最高,CEA水平在腺癌组最高,与其他组比较差异有统计学意义(P<0.01).结论 血清SCCA、CYFRA21-1、CEA检测在肺癌的诊断、病理类型的推断中具有重要意义,而联合检测更有助于肺癌的诊断.%Objective To evaluate the clinical value of combined determination of tumor markers including serum squamous cell carcinoma antigen(SCCA) ,cytokeratin 19 fragment(CYFRA21-l) , carcino-embryonic antigen(CKA) in diagnosis of lung cancer. Methods 63 cases including 44 cases of lung cancer and 19 benign lesion were collected from inpatients registered in our hospital from January to April in 2008. The lung cancer were pathologically diagnosed including 5 cases of small cell lung cancer, 17 cases of squamous cell carcinoma ,15 cases of adenocarcinoma,and 7 undecided type lung cancer. All cases were bronchoalveolar lavaged and bronchoalveolar lavage fluid were collected to prepare for detect SCCA, CYFRA21-1, CEA. Results The levels of the 3 tumor markers measured in serum were higher in lung cancer group than those in benign lung disease group(P<0. 01). The tumor markers increased to different degrees among the patients in various pathological classifications. The levels of SCCA ,CYFRA21-1 measured in serum were higher in squamous cell carcinoma group, and the levels of CEA were higher in adenocarcinoma group(P< 0. 01). Conclusion The

  14. Report on transparency and nuclear safety - Cadarache CEA centre - 2012

    International Nuclear Information System (INIS)

    A first volume proposes a presentation of the Cadarache CEA centre, of its activities and installations, gives a rather detailed overview of measures related to safety and to radiation protection within these activities and installations. It also reports significant events related to safety and to radiation protection which occurred in 2012 and have been declared to the ASN. It discusses the results of release measurements (liquid and gaseous effluents, radiological assessment, and chemical assessment for various installations) and the control of the chemical and radiological impact of these gaseous and liquid effluents on the environment. It addresses the issue of radioactive wastes which are stored in the different nuclear base installations of the Centre, indicates the different measures aimed at limiting the volume of these warehoused wastes and addresses their impact on health and on the environment. Nature and quantities of warehoused wastes are specified. The second volume concerns some specific installations (INB 32 or ATPu, and INB 54 or LPC) which belong to AREVA NC. The same topics are addressed: presentation of the facilities, arrangements regarding safety and radiation protection, significant events related to safety and radiation protection, measurements of effluents and their impact on the environment, warehoused wastes. Remarks and recommendations of the CHSCT are given

  15. CEA distribution transformer purchasing specifications (DTWG-01,02,03)

    International Nuclear Information System (INIS)

    Purchasing specifications for three types of distribution transformers are presented. The specifications were compiled by the Canadian Electricity Association at the suggestion made in 1989 by the Canadian Utilities Material Management Group. The specifications cover pole mounted single phase distribution transformers (DTWG-01), low-profile, single phase, dead-front pad-mounted distribution transformers (DTWG-02), and three phase, dead-front pad-mounted distribution transformers (DTWG-03). The specifications were compiled by a task force of CEA member utilities, using CSA standards as the governing standards in all three cases. The first edition of the purchasing specifications was issued in 1993. The second edition, consisting mainly of revisions based on experiences learned from using the first edition, and the addition of the appropriate clauses of CSA standards, was published in 1998. Based on a three-year average of the number of transformers purchased annually (about 58,000) at an estimated total cost of $ 120 million, use of the Purchasing Specifications is said to have resulted in savings of about 7 per cent or $ 8.4 million

  16. The use and evolution of the CEA research reactors

    International Nuclear Information System (INIS)

    The authors successively examine the different research reactors in use in the French C.E.A. Nuclear Centres. They trace briefly their histories, describing how they have been used up to the present, and how they have been adapted to changes in programme by means of certain modifications. They also describe the reasons which have led to the elaboration of the project for the new reactor Osiris. Zoe, the oldest reactor in the CEA, has been in service in the Centre de Fontenay-aux-Roses since 1948. It is used mainly for measurements of absorption cross-sections in graphite, and for various short irradiations which do not require high fluxes. The reactor EL 2, in service since 1952, was used for the first studies on gas cooling. It has also been widely used for the production of radioisotopes and for a large number of experiments in the fields of physics, metallurgy and physical chemistry. The ageing of certain elements of the reactor has led to the decision to close it down in the near future The reactor EL 3 has been widely used for experiments in physics and in the investigation of fuels. The possibilities of the reactor in fast neutron irradiations will be considerably improved by the adoption of a new type of core (the 'snow crystal' structure). Triton-I, a 2 MW swimming-pool reactor, is used for the most part for fast neutron and gamma irradiations. The modifications being carried out on it at present should result in an increase in the power of the reactor up to 4 or 5 MW. In a neighbouring compartment is housed Triton-II which is of the same general structure, as Triton-I, but whose maximum power is 100 kW. Triton-II is used solely for studies on shielding. Melusine, a 2 MW swimming-pool reactor, has been in use in the Centre d'Etudes Nucleaires de Grenoble since 1959. It has supported a very high programme concerned mainly with solid state physics, fundamental research into refractory fissile materials and special graphites, and the study of the behaviour of

  17. The possibility of complementary CEA/PSI physics experiments

    International Nuclear Information System (INIS)

    The zero-power, PROTEUS, is a versatile critical facility for providing integral data to test calculational methods and data used in the neutronics design of standard and advanced reactor systems. An experimental programme is planned for PROTEUS with the main aim to support the Swiss nuclear power plants in questions addressing the neutron physics of new, highly heterogeneous BWR fuel-assemblies in order to reduce the conservations included in the margins to operational limits, on the one hand, as well as special investigations in MOX recycling LWR cores, on the other hand. A second objective considers R+D-contributions to the neutron physics of advanced LWR fuel cycles. In recent years, experiments relating to MOX recycling in PWRs have been performed on the EOLE facility at the Nuclear Research Centre in Cadarache. A five years programme is now planned for the EOLE facility with the aim to study and validate 100% MOX fuel recycling in PWRs. Some experiments will be performed in the PROTEUS facility in co-operation with CEA to complement the measurements in the EOLE facility, addressing questions such as: - intercomparison of standard experimental techniques to reduce systematic errors, - data validation through measurements of non-standard reaction rates in normal, under- and over-moderated LWR-spectra, - void simulation experiments using special techniques, - studies on the reduction of radiation damage on the reactor pressure vessel for 100% MOX cores, by introducing steel baffles between the core boundary and the reactor pressure vessel. (author) 2 figs., 10 refs

  18. 肺癌抗原标志物联合检测的临床应用%Clinical application of joint detection with lung cancer antigen markers

    Institute of Scientific and Technical Information of China (English)

    温仁祝; 戴磊; 张亚男

    2015-01-01

    Objective To explore the best combination of joint detection with lung cancer antigen markers . Methods A random sample of 200 patients diagnosed with primary lung cancer patients ,measuring the level of a wide variety of tumor antigen markers in the blood and statistical analysis . Results In the detection of multiple tumor markers CEA ,NSE ,CYFRA21‐1 and CA125 positive rate is relatively high;serum markers in the expression of related to clinical stage . Conclusion The combined detection of CEA and CA125 ,NSE ,CYFRA21‐1 can im‐prove the accuracy of clinical diagnosis of lung cancer patients .%目的:探讨肺癌抗原标志物联合检测的最佳组合。方法随机抽取200例确诊为原发性肺癌的患者,测定患者血液中多种肿瘤抗原标志物的水平,并进行统计学分析。结果检测的多项肿瘤标志物中CEA、NSE、CYFRA21‐1和CA125的阳性率相对较高;血清各标志物的表达与临床分期有关。结论联合检测CEA、NSE、CYFRA21‐1和CA125能提高肺癌患者的临床诊断准确率。

  19. The CEA Cadarache site. Additional safety assessment with respect to the accident which occurred in the Fukushima-Daiichi nuclear power station

    International Nuclear Information System (INIS)

    After a presentation of some characteristics of the CEA Cadarache site (internal and external industrial environment, crisis management organization at the CEA level and at the local level), this document reports the identification of structures and equipment concerned by crisis management (site support functions, critical structures and equipment concerned by additional safety assessments). Then, it addresses the different risks: earthquake (sizing of critical structures and equipment, margin assessment), flooding (possible origins, alarm measures), other extreme natural events (extreme meteorological conditions, extreme earthquake with induced flooding, forest fire), and loss of electric supplies and of cooling systems. The last parts address the organization of accident management in situation typically related to additional safety management), and subcontracting conditions and practices

  20. The contract State-CEA 2001-2004 for the civil activities; Le contrat etat-Cea 2001-2004 pour les activites civile

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-19

    The 25 january 2001, the CEA signed its pluri-annual contract with the State. Apply some to the complete document, this paper deals with the part concerning the energy. The main objective concerns the radioactive wastes management and the mastership of the nuclear activities impacts. The other part is devoted to the research programs and the technology for the industrial innovation. In this framework the CEA have to develop its program on the new following technologies: hydrogen and fuel cells, photovoltaic and storage, energy conservation. A part of the contract concerns also the fundamental research on spallation and transmutation. (A.L.B.)

  1. Network and system diagrams revisited: Satisfying CEA requirements for causality analysis

    International Nuclear Information System (INIS)

    Published guidelines for Cumulative Effects Assessment (CEA) have called for the identification of cause-and-effect relationships, or causality, challenging researchers to identify methods that can possibly meet CEA's specific requirements. Together with an outline of these requirements from CEA key literature, the various definitions of cumulative effects point to the direction of a method for causality analysis that is visually-oriented and qualitative. This article consequently revisits network and system diagrams, resolves their reported shortcomings, and extends their capabilities with causal loop diagramming methodology. The application of the resulting composite causality analysis method to three Environmental Impact Assessment (EIA) case studies appears to satisfy the specific requirements of CEA regarding causality. Three 'moments' are envisaged for the use of the proposed method: during the scoping stage, during the assessment process, and during the stakeholder participation process

  2. Comparison of Ablation Predictions for Carbonaceous Materials Using CEA and JANAF-Based Species Thermodynamics

    Science.gov (United States)

    Milos, Frank S.

    2011-01-01

    In most previous work at NASA Ames Research Center, ablation predictions for carbonaceous materials were obtained using a species thermodynamics database developed by Aerotherm Corporation. This database is derived mostly from the JANAF thermochemical tables. However, the CEA thermodynamics database, also used by NASA, is considered more up to date. In this work, the FIAT code was modified to use CEA-based curve fits for species thermodynamics, then analyses using both the JANAF and CEA thermodynamics were performed for carbon and carbon phenolic materials over a range of test conditions. The ablation predictions are comparable at lower heat fluxes where the dominant mechanism is carbon oxidation. However, the predictions begin to diverge in the sublimation regime, with the CEA model predicting lower recession. The disagreement is more significant for carbon phenolic than for carbon, and this difference is attributed to hydrocarbon species that may contribute to the ablation rate.

  3. Numerical Platon: A unified linear equation solver interface by CEA for solving open foe scientific applications

    International Nuclear Information System (INIS)

    This paper describes a tool called 'Numerical Platon' developed by the French Atomic Energy Commission (CEA). It provides a freely available (GNU LGPL license) interface for coupling scientific computing applications to various freeware linear solver libraries (essentially PETSc, SuperLU and HyPre), together with some proprietary CEA solvers, for high-performance computers that may be used in industrial software written in various programming languages. This tool was developed as part of considerable efforts by the CEA Nuclear Energy Division in the past years to promote massively parallel software and on-shelf parallel tools to help develop new generation simulation codes. After the presentation of the package architecture and the available algorithms, we show examples of how Numerical Platon is used in sequential and parallel CEA codes. Comparing with in-house solvers, the gain in terms of increases in computation capacities or in terms of parallel performances is notable, without considerable extra development cost

  4. Scientific evaluation at the French Atomic Energy Commission (CEA). 2005-2006 annual report

    International Nuclear Information System (INIS)

    This report aims at presenting the scientific evaluation activities carried out at the CEA during the years 2005-2006. The evaluation system is described in detail. It comprises two aspects: the evaluation of the scientific policy implemented by the scientific Council and by the visiting committee of the CEA, and the evaluation of the laboratories, performed by a pool of 36 scientific councils. The evaluation by external and independent parties is the key point of this system. This document makes a status of the evaluations performed in 2005 and 2006. It presents a synthesis of the conclusions of the evaluation authorities. The actions implemented by the CEA to take into consideration the recommendations are also reported with the improvements noticed. The two topics examined by the scientific Committee and by the visiting committee were dealing with the energy domain, which is a strategic issue for the CEA. The examination of the researches on future nuclear reactors and on new energy technologies have shown the major role played by the CEA in the recent advances in these domains. About 95% of the laboratories activity was examined during the 2002-2005 period. The richness of the remarks and recommendations made by the scientific councils should allow the CEA to improve the quality and relevance of its research works. The start-up of the 2006-2009 evaluation cycle has been the occasion to modify the evaluation of some research domains in order to take into account the evolution of programs. The evaluation system of the CEA is highly consistent with the AERES principles. Its implementation, adapted to each type of activity (fundamental research, applied research, technological developments) allows the CEA to follow up a permanent improvement approach. (J.S.)

  5. Integral needs for MOX powders: state of the art at Cea Cadarache on MOX fuel experiments

    International Nuclear Information System (INIS)

    Several experimental programmes have been conducted at CEA connected with the use of MOX in commercial PWR or innovative concepts, such as high conversion light water reactors (HCLWRs) and at various moderation ratios. Measurements of integral parameters of interest were performed using fission chambers and gamma-scanning techniques, or by oscillation techniques. This paper presents an overview of the experimental programmes performed at the EOLE and MINERVE facilities located at CEA Cadarache. (author)

  6. Unusual elevation of CEA in a patient with history of colon cancer.

    Science.gov (United States)

    Hsu, Tzu-Chi

    2006-12-01

    A 35-year-old female received right hemicolectomy for a poorly differentiated adenocarcinoma of the ascending colon with lymph node metastasis (1/28) in February 1997. CEA was 1.68 ng/microl prior to colectomy. Adjuvant chemotherapy with weekly 5-FU and leucovorin intravenously was started following surgery and discontinued after 17 doses in May 1997. She received bilateral salpingo-ophorecctomy for metastatic cancer in August 1999. Intravenous chemotherapy was resumed with weekly 5-FU and leucovorin intravenously in August 1999. CEA was 93.8 ng/microl in November 1999. Intravenous chemotherapy was discontinued after 20 doses and oral chemotherapy with futraful and leucovorin was started in January 2000. CEA was found to be 240.3 ng/microl in December 1999 and then elevated to 1521.3 ng/microl in June 2001, which was 10 months after resection of metastatic ovarian cancer. No metastatic lesions could be detected, however, with image studies. The CEA decreased to 396.6 ng/microl three months later. Futraful was switched to uracil-tegafur (UFUR) in September 2001. The CEA for the patient ranged from 68.5 to 298.9 ng/microl for the following 5 years without aggressive chemotherapy. No evidence of recurrence could be demonstrated by imaging studies. The patient is not a smoker and denied exposure to a smoking environment. She was also not known to have persistent infections, inflammatory bowel disease, pancreatitis, cirrhosis of the liver, or any benign tumors. The current case suggested that: (i) elevation of CEA is not necessarily well correlated with presence of metastatic colon cancer; (ii) some patients may live with elevated CEA for years without evidence of recurrence or metastasis; (iii) aggressive chemotherapy may not be necessary in patients with only elevated CEA. PMID:17060406

  7. CEA, a Major Player in Technological Innovation for Defense and Security

    International Nuclear Information System (INIS)

    The Secure Day Conference held in Paris on October 24, 2012 was organized by CEA with the support of the French High Committee for Civil Defense (Haut Comite Francais pour la Defense Civile - HCFDC). The event gathered more than 250 people (institutions, users and industrial companies) and alternated conferences and presentations on about thirty technologies developed in the framework of joint ministerial CBRN-E R and D program entrusted to CEA by public authorities. (authors)

  8. Detection of prostate specific antigen in serum at the femto-gram per milliliter level using the intrinsic amplification of a field-effect enzymatic immuno-sensing system

    International Nuclear Information System (INIS)

    Ultrasensitive detection of prostate specific antigen (PSA) has been achieved by applying the field-effect enzymatic detection (FEED) technique to the sandwich immuno-sensing technique. The voltage-controlled intrinsic amplification provided by FEED enabled the detection of PSA contained in serum on the femto-gram/mL level. Two electrochemical approaches used to obtain the amperometric detection signal resulted in similar detection limits and sensitivities. The lowest PSA detection limit achieved was 27 fg mL−1. The high selectivity of the detection system was reflected in the fact that PSA detection was successful on the fg mL−1 level, where biological substances other than PSA had a 1-million-fold higher concentration. Electron transfer through the immunological sandwich nanostructure has been observed in the detection of biomarkers. However, our results showed that electron transfer through the nanostructure could be controlled using an external voltage, leading to an ultralow detection limit for PSA

  9. Nano-iniferter based imprinted sensor for ultra trace level detection of prostate-specific antigen in both men and women.

    Science.gov (United States)

    Patra, Santanu; Roy, Ekta; Madhuri, Rashmi; Sharma, Prashant K

    2015-04-15

    In this work, a sensitive and selective electrochemical sensor for detection of prostate specific antigen (PSA) was developed using surface imprinting and nanotechnology. The multiwalled carbon nanotubes (MWCNTs) decorated with manganese nanoparticles was functionalized with thio-group to make a nano-iniferter. This nano-iniferter was used as a platform to synthesize a three-dimensional molecularly imprinted polymer matrix for PSA by controlled radical polymerization technique. The PSA-sensor displayed good analytical performance for the detection of PSA by square wave and differential pulse stripping voltammetric techniques. The limit of detection was calculated as low as 0.25pgL(-1) (from SWSV) and 3.04pgL(-1) (from DPSV) at signal to noise ratio of 3. The proposed sensor was successfully applied for the determination of PSA in human blood serum, urine, and forensic samples without any cross-reactivity. Thus this sensor offers high selectivity, sensitivity, simplicity and clinical applicability for PSA determination in sera of both the men and women, which have not been reported together in the previously reported systems. The sensor may be used as a better alternative for the commercially available ELISA kits for PSA determination. PMID:25460874

  10. 晚期非小细胞肺癌患者血清癌胚抗原水平和全身转移能力的风险评估%Serum Level of Carcinoembryonic Antigen and Whole-body Metastasis Ability Risk Assessment in Patients with Advanced Non-small Cell Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    李捷; 李付广; 常保萍; 焦江琴

    2015-01-01

    目的:探究晚期非小细胞肺癌(NSCLC)患者血清癌胚抗原水平和全身转移能力的相关性。方法:选取2012年3月-2014年1月来本院治疗的初治IV期NSCLC患者160例为研究对象。研究指标包括年龄、身高、体重、性别、组织学分类、肿瘤分化、CEA水平、吸烟状况、骨转移、淋巴结转移、胸膜/积液转移、颈部转移、软组织转移、脑转移、全身转移得分及LN-调整全身转移得分。结果:不同CEA水平患者的性别、组织学分类、骨转移、淋巴结转移和脑转移比较差异具有统计学意义(P0.05)。血清CEA水平从LN-调整全身转移得分1分中的2.7 ng/mL上升至LN-调整全身转移得分7分中的374.1 ng/mL(P=0.003)。低LN-调整全身转移得分(评分1~3分)与高LN-调整全身转移得分(评分4~7分)比较表现出CEA水平显著降低,两组比较差异具有统计学意义(P0.05).Serum CEA level increased from 2.7 ng/mL in LN-adjusted whole-body metastatic score 1 to 374.1 ng/mL in LN-adjusted whole-body metastatic score 7,the difference was statistically significant (P=0.003).Similarly, the low LN-adjusted whole-body metastatic score (rating 1-3) showed CEA level significantly reduced when compared with adjustment of high LN-adjusted whole-body metastatic score (rating 4-7),the difference was statistically significant(P<0.001).Scores of high CEA levels in serum from 37.5%(15/40) in LN-adjusted whole-body metastatic score 1 rose to 100%(1/1) in LN-adjusted whole-body metastatic score 6, the difference was statistically significant(P<0.001).Similarly, high serum CEA score rose from 25% (2/8) in LN-adjusted whole-body metastatic score 1 rose to 100%(1/1) in LN-adjusted whole-body metastaticscore 7, the difference was statistically significant(P<0.001).Univariate analysis results showed that gender, bone metastases, histological classification and lymph node metastases and brain metastasis were risk factors of

  11. Melanoma cell surface-expressed phosphatidylserine as a therapeutic target for cationic anticancer peptide, temporin-1CEa.

    Science.gov (United States)

    Wang, Che; Chen, Yin-Wang; Zhang, Liang; Gong, Xian-Ge; Zhou, Yang; Shang, De-Jing

    2016-07-01

    We have previously reported that temporin-1CEa, a cationic antimicrobial peptide, exerts preferential cytotoxicity toward cancer cells. However, the exact molecular mechanism for this cancer-selectivity is still largely unknown. Here, we found that the negatively charged phosphatidylserine (PS) expressed on cancer cell surface serves as a target for temporin-1CEa. Our results indicate that human A375 melanoma cells express 50-fold more PS than non-cancerous HaCaT cells. The expression of cell surface PS in various cancer cell lines closely correlated with their ability to be recognized, bound and killed by temporin-1CEa. Additionally, the cytotoxicity of temporin-1CEa against A375 cells can be ameliorated by annexin V, which binds to cell surface PS with high affinity. Moreover, the data of isothermal titration calorimetry assay further confirmed a direct binding of temporin-1CEa to PS, at a ratio of 1:5 (temporin-1CEa:PS). Interestingly, the circular dichroism spectra analysis using artificial biomembrane revealed that PS not only provides electrostatic attractive sites for temporin-1CEa but also confers the membrane-bound temporin-1CEa to form α-helical structure, therefore, enhances the affinity and membrane disrupting ability of temporin-1CEa. In summary, these findings suggested that the melanoma cells expressed PS may serve as a promising target for temporin-1CEa or other cationic anticancer peptides. PMID:26596643

  12. Towards allele-level human leucocyte antigens genotyping - assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq.

    Science.gov (United States)

    Duke, J L; Lind, C; Mackiewicz, K; Ferriola, D; Papazoglou, A; Derbeneva, O; Wallace, D; Monos, D S

    2015-10-01

    Human leucocyte antigens (HLA) typing has been a challenge due to extreme polymorphism of the HLA genes and limitations of the current technologies and protocols used for their characterization. Recently, next-generation sequencing techniques have been shown to be a well-suited technology for the complete characterization of the HLA genes. However, a comprehensive assessment of the different platforms for HLA typing, describing the limitations and advantages of each of them, has not been presented. We have compared the Ion Torrent Personal Genome Machine (PGM) and Illumina MiSeq, currently the two most frequently used platforms for diagnostic applications, for a number of metrics including total output, quality score per position across the reads and error rates after alignment which can all affect the accuracy of HLA genotyping. For this purpose, we have used one homozygous and three heterozygous well-characterized samples, at HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1. The total output of bases produced by the MiSeq was higher, and they have higher quality scores and a lower overall error rate than the PGM. The MiSeq also has a higher fidelity when sequencing through homopolymer regions up to 9 bp in length. The need to set phase between distant polymorphic sites was more readily achieved with MiSeq using paired-end sequencing of fragments that are longer than those obtained with PGM. Additionally, we have assessed the workflows of the different platforms for complexity of sample preparation, sequencer operation and turnaround time. The effects of data quality and quantity can impact the genotyping results; having an adequate amount of good quality data to analyse will be imperative for confident HLA genotyping. The overall turnaround time can be very comparable between the two platforms; however, the complexity of sample preparation is higher with PGM, while the actual sequencing time is longer with MiSeq. PMID:26119888

  13. Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen

    Directory of Open Access Journals (Sweden)

    Ross Anton

    2009-02-01

    Full Text Available Abstract Background Hepatitis B is a serious global public health concern. Though a safe and efficacious recombinant vaccine is available, its use in several resource-poor countries is limited by cost. We have investigated the production of Hepatitis B virus surface antigen (HBsAg using the yeast Pichia pastoris GS115 by inserting the HBsAg gene into the alcohol oxidase 1 locus. Results Large-scale production was optimized by developing a simple fed-batch process leading to enhanced product titers. Cells were first grown rapidly to high-cell density in a batch process using a simple defined medium with low salt and high glycerol concentrations. Induction of recombinant product synthesis was carried out using rather drastic conditions, namely through the addition of methanol to a final concentration of 6 g L-1. This methanol concentration was kept constant for the remainder of the cultivation through continuous methanol feeding based on the on-line signal of a flame ionization detector employed as methanol analyzer in the off-gas stream. Using this robust feeding protocol, maximum concentrations of ~7 grams HBsAg per liter culture broth were obtained. The amount of soluble HBsAg, competent for assembly into characteristic virus-like particles (VLPs, an attribute critical to its immunogenicity and efficacy as a hepatitis B vaccine, reached 2.3 grams per liter of culture broth. Conclusion In comparison to the highest yields reported so far, our simple cultivation process resulted in an ~7 fold enhancement in total HBsAg production with more than 30% of soluble protein competent for assembly into VLPs. This work opens up the possibility of significantly reducing the cost of vaccine production with implications for expanding hepatitis B vaccination in resource-poor countries.

  14. Radioimmunometric assay of carbohydrate antigen 549 in diagnosis and follow-up of breast carcinoma

    International Nuclear Information System (INIS)

    Aim: The aim of this study is to assess the value of carbohydrate antigen 549 (CA 549) in diagnosis and follow-up of breast carcinoma and compare CA 549 with other tumoral markers. Methods: A. We have studied 1230 determinations of CA 549 corresponding to 460 patients (3 male) with confirmed or suspected diagnosis of breast carcinoma. CA 549 was determined using a radioimmunometric assay that use a 'sandwich' technique on solid phase. B. To compare CA 549 with other tumoral markers we have reviewed the case history of 40 patients with advanced breast carcinoma. The tumoral markers studied were: CEA, CA 19.9, CA 15.3 and CA 549. C. We have performed a correlation study between the different tumoral markers on 124 evaluations and calculated the Pearson's correlation coefficient. Results: In the group of confirmed or suspected diagnosis of breast carcinoma (n=460): 187 patients (40,65%) showed pathological levels of CA 549. In the group of patients with advanced breast carcinoma (36 patients with metastasis and 4 with advanced local extension). the results of the different tumoral markers are presented. The Pearson's correlation coefficient between CA 549 and CA 15.3 was 0.814, while no correlation was found in the remaining markers. Conclusion: CA 549 is a good marker of progression or persistence of tumoral disease in breast carcinoma

  15. Three-dimensional conformal external beam radiotherapy compared with permanent prostate implantation in low-risk prostate cancer based on endorectal magnetic resonance spectroscopy imaging and prostate-specific antigen level

    International Nuclear Information System (INIS)

    Purpose: To evaluate the metabolic response by comparing the time to resolution of spectroscopic abnormalities (TRSA) and the time to prostate-specific antigen level in low-risk prostate cancer patients after treatment with three-dimensional conformal external beam radiotherapy (3D-CRT) compared with permanent prostate implantation (PPI). Recent studies have suggested that the treatment of low-risk prostate cancer yields similar results for patients treated with 3D-CRT or PPI. Methods and Materials: A total of 50 patients, 25 in each group, who had been treated with 3D-CRT or PPI, had undergone endorectal magnetic resonance spectroscopy imaging before and/or at varying times after therapy. The 3D-CRT patients had received radiation doses of ≥72 Gy compared with 144 Gy for the PPI patients. The spectra from all usable voxels were examined for detectable levels of metabolic signal, and the percentages of atrophic and cancerous voxels were tabulated. Results: The median time to resolution of the spectroscopic abnormalities was 32.2 and 24.8 months and the time to the nadir prostate-specific antigen level was 52.4 and 38.0 months for the 3D-CRT and PPI patients, respectively. Of the 3D-CRT patients, 92% achieved negative endorectal magnetic resonance spectroscopy imaging findings, with 40% having complete metabolic atrophy. All 25 PPI patients had negative endorectal magnetic resonance spectroscopy imaging findings, with 60% achieving complete metabolic atrophy. Conclusion: The results of this study suggest that metabolic and biochemical responses of the prostate are more pronounced after PPI. Our results have not proved PPI is more effective at curing prostate cancer, but they have demonstrated that it may be more effective at destroying prostate metabolism

  16. Construction, Expression and Characterization of a Chimeric Protein Targeting Carcinoembryonic Antigen in Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    LI Yang; HUA Shu-cheng; MA Cheng-yuan; YU Zhen-xiang; XU Li-jun; LI Dan; SUN Li-li; LI Xiao; PENG Li-ping

    2011-01-01

    The carcinoembryonic antigen(CEA) is an oncofetal glycoprotein known as an important clinical tumor marker and is overexpressed in several types of tumors, including colorectal and lung carcinomas. We constructed a chimeric protein that exhibits both specific binding and immune stimulating activities, by fusing staphylococcal enterotoxin A(SEA) to the C-terminus of an anti-CEA single-chain disulfide-stabilized Fv(scdsFv) antibody (single-chain-C-terminus/SEA, SC-C/SEA). The SC-C/SEA protein was expressed in Escherichia coli(E. coli), refolded, and purified on an immobilized Ni2+ affinity chromatography column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blot analysis reveal that the target protein was expressed sufficiently. We used immunofluorescence assays to demonstrate that SC-C/SEA could bind specifically to human lung carcinoma cells(A549), but almost human uterine cervix cells(HeLa). We also used the L-lactate dehydrogenase(LDH) release assay to show that SC-C/SEA elicits a strong A549 tumor-specific cytotoxic T lymphocyte(CTL) response in vitro. The results suggest that SC-C/SEA shows specific activity against CEA-positive cells and has potential application in CEA-targeted cancer immunotherapy.

  17. Technical report on the fatigue crack Growth Benchmark based on CEA pipe bending tests

    International Nuclear Information System (INIS)

    In order to improve the estimation methods of surface crack propagation through the thickness of components, CEA has proposed a benchmark to members of the IAGE WG, sub-group on Integrity of metal components and structures. The subject is a simple configuration of a pipe containing an axisymmetric notch and submitted to a cyclic bending load. An experimental data-set form CEA was used to validate three issues in the topic of Leak Before Break. - Crack initiation, - Crack propagation through the thickness, - Crack penetration. All material and geometrical data which are necessary for the simulation were given in the proposal, including experimental results. Due to the peculiar complexity of the problem, it was decided to focus the work on methodologies comparison so as to allow participants to tune up parameters and adjust their models and tools. This report presents all estimations performed by the participants and collected by CEA. They are compared to the experimental results. An analysis of the used procedures is also proposed. This, associated with the study of the accuracy of different methodologies, leads to comments and recommendations on the analysis of fatigue crack growth. The participation in the first step was important: nine participants have proposed analyses, sometimes parametric analysis to estimate crack growth. Results sorted out three estimation methods groups that give results in accordance with experimental ones (these three groups are based on a strain range evaluation and the fatigue curve of the material): - The use of an elastic stress at the notch tip and a fatigue notch concentration factor to determine the strain range. - The use of a KI (or elastic F.E. calculation) and a Neuber rule for the estimation of the strain range at a characteristic distance from the crack tip. - The direct calculation of the strain range at the characteristic distance by an elastic plastic F.E. calculation. Only 4 participants have proposed an estimate of the

  18. Presentation of the CEA's crisis national organization: coordination centre in case of crisis, crisis technical teams, intervention means, and so on

    International Nuclear Information System (INIS)

    After having briefly recalled the existence of a legal framework for crisis management organisation, this report briefly describes how the CEA plans the crisis management. This management is based on the definition of critical scenarios, on the building up of a crisis management team, and on the elaboration of crisis management operational documents. It evokes the alert organisation and the triggering of crisis management. Then, it describes the CEA's national crisis organisation with its main crisis management structures, the role and the operation of the Crisis Coordination Centre (CCC, the decision body), the role and operation of the Central Crisis Technical Teams (ETC-C, Equipes Techniques de Crise Centrales), the role of field interveners (various rescue, protection, health care and technical teams) and of other additional intervention actors. It evokes the objectives of the various exercises which are organised every year at the internal, national or international level

  19. Peripheral blood lymphocytes from low-grade squamous intraepithelial lesions patients recognize vaccine antigens in the presence of activated dendritic cells, and produced high levels of CD8 + IFNγ + T cells and low levels of IL-2 when induced to proliferate

    Directory of Open Access Journals (Sweden)

    Hernández-Montes Jorge

    2012-05-01

    Full Text Available Abstract Background Most infections with human papillomavirus (HPV are resolved without clinical intervention, but a minority evolves into chronic lesions of distinct grades, including cervical-uterine cancer. It is known that in most cases the immune system mediates elimination of HPV infection. However, the mechanism of immune evasion leading to HPV persistence and development of early cervical lesions is not fully understood. The aim of the present work was to evaluate the potential of peripheral blood leukocytes (PBL from low-grade squamous intraepithelial lesions (LSIL patients to be activated ex-vivo by vaccine antigens, the participation of cytotoxic lymphocytes and regulatory T cells, and to determine the secretion of Th1 and Th2 cytokines mediated by stimulation of T cell receptors. Results We found that PBL from LSIL patients showed a significantly lower proliferation rate to vaccine antigens as compared to that of healthy donors, even though there was not a difference in the presence of antibodies to those antigens in sera from both groups. We did not find differences in either the frequency of CD4 + CD25 + FoxP3+ in PBL, or the levels of IL-4, IL-5 and IL-10 in plasma or conditioned media from PBL incubated with TcR agonists in vitro, between the two groups. However, we detected a lower production of IL-2 and a higher proportion of CD8 + IFNγ + cells in PBL from LSIL patients as compared with PBL from normal donors. We also observed that PBL from patients infected by HPV-16 and −18 were not able to proliferate in the presence of soluble HPV antigens added to the culture; however, a high level of proliferation was attained when these antigens were presented by activated dendritic cells. Conclusions Our results suggest that the immunodeficiency reported in LSIL patients could be due to the inability of specific cytotoxic T lymphocytes that for some unknown reason are present but unable to mount a response when

  20. A Sandwich Electrochemical Immunosensor Using Magnetic DNA Nanoprobes for Carcinoembryonic Antigen

    Directory of Open Access Journals (Sweden)

    Ning Gan

    2011-10-01

    Full Text Available A novel magnetic nanoparticle-based electrochemical immunoassay of carcinoembryonic antigen (CEA was designed as a model using CEA antibody-functionalized magnetic beads [DNA/Fe3O4/ZrO2; Fe3O4 (core/ZrO2 (shell nano particles (ZMPs] as immunosensing probes. To design the immunoassay, the CEA antibody and O-phenylenediamine (OPD were initially immobilized on a chitosan/nano gold composite membrane on a glassy carbon electrode (GCE/CS-nano Au, which was used for CEA recognition. Then, horseradish peroxidase (HRP-labeled anti-CEA antibodies (HRP-CEA Ab2 were bound to the surface of the synthesized magnetic ZMP nanoparticles as signal tag. Thus, the sandwich-type immune complex could be formed between secondary antibody (Ab2 modified DNA/ZMPs nanochains tagged by HRP and GCE/CS-nano Au. Unlike conventional nanoparticle-based electrochemical immunoassays, the recognition elements of this immunoassay included both electron mediators and enzyme labels, which obviously simplifies the electrochemical measurement process. The sandwich-type immunoassay format was used for online formation of the immunocomplex of CEA captured in the detection cell with an external magnet. The electrochemical signals derived from HRP during the reduction of H2O2 with OPD as electron mediator were measured. The method displayed a high sensitivity for CEA detection in the range of 0.008–200 ng/mL, with a detection limit of 5 pg/mL (estimated at a signal-to-noise ratio of 3. The precision, reproducibility, and stability of the immunoassay were good. The use of the assay was evaluated with clinical serum samples, and the results were in excellent accordance with those obtained using the standard enzyme-linked immunosorbent assay (ELISA method. Thus, the magnetic nanoparticle-based assay format is a promising approach for clinical applications, and it could be further developed for the detection of other biomarkers in cancer diagnosis.

  1. Report on transparency and nuclear safety - Fontenay-aux-Roses CEA centre - 2012

    International Nuclear Information System (INIS)

    This report presents the different nuclear base installations (INB) of the Fontenay-aux-Roses CEA centre, gives an overview of measures regarding safety within these installations (organisation, general arrangements, arrangements related to different risks, defence in-depth, management of emergency situations, inspections, audits and second-level controls, arrangements and main events specific to the different installations and buildings, issues related to transports, soil radiological assessment) and measures related to radiation protection (organisation and results). It reports the significant events related to safety and radiation protection which occurred in 2012 and were declared to the ASN, and discusses how the return-on-experience has been used. It reports and comments the results of measurements of radiological and chemical gaseous and liquid effluents, of surveys of the environment. It also evokes important events related to these measurement and survey processes, presents the environmental management approach. The next part addresses the management of radioactive wastes: arrangements aimed at limiting the volume of warehoused wastes, and at limiting their impact on health and on the environment, nature and quantities of warehoused wastes. The different arrangements regarding transparency and information are reviewed (TSN report, newsletter, and so on)

  2. Training and Certification Program for Certified Energy Auditors (CEA) and Certified Building Commissioning Professionals (CBCP)

    Energy Technology Data Exchange (ETDEWEB)

    Kent, Bill

    2012-08-24

    The Association of Energy Engineers (AEE) has offered energy efficiency training and certification programs for over 30 years. During that time AEE has certified more than 22,000 professionals. All of our certification programs are the result of extensive industry research and program development and oversight by certification boards. For this project award, AEE proposed to work with the Department of Energy to utilize and extend existing industry recognized Certified Energy Auditor (CEA) and Certified Building Commissioning Professional (CBCP) programs under this Training Program Development Announcement. These expanded training programs will have significant impact in training professionals for building commissioning and energy auditing to achieve the goal of bringing existing buildings up to their optimal energy performance potential and ensuring that new buildings maintain their expected optimal level of performance. The goals and objectives of the training development project were achieved with the development of new training programs that are now being offered as self-sustaining commercial training and certification programs. These new programs are training and certifying professionals who are accomplishing the goal of increasing building energy performance in both existing and new buildings.

  3. METABOLISMO ÁCIDO DE LAS CRASULÁCEAS Crassulacean Acid Metabolism

    Directory of Open Access Journals (Sweden)

    THOMAS DAVID GEYDAN

    Full Text Available Se presenta una revisión del metabolismo ácido de las Crasuláceas, caracterizado por la ocurrencia, actividad y plasticidad del mecanismo desde un punto de vista fisiológico, bioquímico y molecular, enmarcado por la presencia de las denominadas cuatro fases de dicho metabolismo y su repercusión y expresión por diversas restricciones hídricas a nivel ecológico. Se presentan las principales enzimas y metabolitos básicos para el funcionamiento del metabolismo CAM, así como su modo de acción y control celular. Finalmente, se muestra que la plasticidad fenotípica en patrones de expresión CAM se encuentra mediada por condiciones ambientales y por señalizaciones moleculares.A review of Crassulacean acid metabolism is presented, characterized by showing the occurrence, activity and plasticity of these complex mechanism at the physiological, biochemical and molecular level, framed by the presence of the denominated four phases in CAM and its repercussion and expression due to different stresses in an ecological context. The basic enzymes, and metabolites necessary for the optional functioning of CAM are presented as well as their mode of action and cellular control. Finally, it is shown how environmental conditions and molecular signalling mediate the phenotypic plasticity.

  4. Report on transparency and nuclear safety - INB unexploited by the Marcoule CEA - 2012

    International Nuclear Information System (INIS)

    This report presents the different nuclear base installations (INB) of the Marcoule CEA centre, gives an overview of measures regarding safety within these installations (organisation, general arrangements, arrangements related to different risks, management of emergency situations, inspections, audits and second-level controls, arrangements and main events specific to the different installations and buildings) and of measures related to radiation protection (organisation and results, main events). It reports the significant events related to safety and radiation protection which occurred in 2012 and were declared to the ASN, and discusses how the return-on-experience has been used. It reports and comments the results of measurements of gaseous and liquid effluents, of their impact on the environment, and of surveys of the environment. It also presents the environmental management approach. The next part addresses the management of radioactive wastes which are warehoused on this site: arrangements aimed at limiting their volume, and at limiting their impact on health and on the environment, nature and quantities of warehoused wastes. Remarks and recommendations of the CHSCT are given

  5. Malaria resistance genes are associated with the levels of IgG subclasses directed against Plasmodium falciparum blood-stage antigens in Burkina Faso

    Directory of Open Access Journals (Sweden)

    Afridi Sarwat

    2012-09-01

    Full Text Available Abstract Background HBB, IL4, IL12, TNF, LTA, NCR3 and FCGR2A polymorphisms have been associated with malaria resistance in humans, whereas cytophilic immunoglobulin G (IgG antibodies are thought to play a critical role in immune protection against asexual blood stages of the parasite. Furthermore, HBB, IL4, TNF, and FCGR2A have been associated with both malaria resistance and IgG levels. This suggests that some malaria resistance genes influence the levels of IgG subclass antibodies. Methods In this study, the effect of HBB, IL4, IL12, TNF, LTA, NCR3 and FCGR2A polymorphisms on the levels of IgG responses against Plasmodium falciparum blood-stage extract was investigated in 220 individuals living in Burkina Faso. The Pearson’s correlation coefficient among IgG subclasses was determined. A family-based approach was used to assess the association of polymorphisms with anti-P. falciparum IgG, IgG1, IgG2, IgG3 and IgG4 levels. Results After applying a multiple test correction, several polymorphisms were associated with IgG subclass or IgG levels. There was an association of i haemoglobin C with IgG levels; ii the FcγRIIa H/R131 with IgG2 and IgG3 levels; iii TNF-863 with IgG3 levels; iv TNF-857 with IgG levels; and, v TNF1304 with IgG3, IgG4, and IgG levels. Conclusion Taken together, the results support the hypothesis that some polymorphisms affect malaria resistance through their effect on the acquired immune response, and pave the way towards further comprehension of genetic control of an individual’s humoral response against malaria.

  6. Immunogenic salivary proteins of Triatoma infestans: development of a recombinant antigen for the detection of low-level infestation of triatomines.

    Directory of Open Access Journals (Sweden)

    Alexandra Schwarz

    Full Text Available BACKGROUND: Triatomines are vectors of Trypanosoma cruzi, the etiological agent of Chagas disease in Latin America. The most effective vector, Triatoma infestans, has been controlled successfully in much of Latin America using insecticide spraying. Though rarely undertaken, surveillance programs are necessary in order to identify new infestations and estimate the intensity of triatomine bug infestations in domestic and peridomestic habitats. Since hosts exposed to triatomines develop immune responses to salivary antigens, these responses can be evaluated for their usefulness as epidemiological markers to detect infestations of T. infestans. METHODOLOGY/PRINCIPAL FINDINGS: T. infestans salivary proteins were separated by 2D-gel electrophoresis and tested for their immunogenicity by Western blotting using sera from chickens and guinea pigs experimentally exposed to T. infestans. From five highly immunogenic protein spots, eight salivary proteins were identified by nano liquid chromatography-electrospray ionization-tandem mass spectrometry (nanoLC-ESI-MS/MS and comparison to the protein sequences of the National Center for Biotechnology Information (NCBI database and expressed sequence tags of a unidirectionally cloned salivary gland cDNA library from T. infestans combined with the NCBI yeast protein sub-database. The 14.6 kDa salivary protein [gi|149689094] was produced as recombinant protein (rTiSP14.6 in a mammalian cell expression system and recognized by all animal sera. The specificity of rTiSP14.6 was confirmed by the lack of reactivity to anti-mosquito and anti-sand fly saliva antibodies. However, rTiSP14.6 was recognized by sera from chickens exposed to four other triatomine species, Triatoma brasiliensis, T. sordida, Rhodnius prolixus, and Panstrongylus megistus and by sera of chickens from an endemic area of T. infestans and Chagas disease in Bolivia. CONCLUSIONS/SIGNIFICANCE: The recombinant rTiSP14.6 is a suitable and promising

  7. Evaluation of the reliability concerning the identification of human factors as contributing factors by a computer supported event analysis (CEA)

    International Nuclear Information System (INIS)

    The project's objectives are the evaluation of the reliability concerning the identification of Human Factors as contributing factors by a computer supported event analysis (CEA). CEA is a computer version of SOL (Safety through Organizational Learning). Parts of the first step were interviews with experts from the nuclear power industry and the evaluation of existing computer supported event analysis methods. This information was combined to a requirement profile for the CEA software. The next step contained the implementation of the software in an iterative process of evaluation. The completion of this project was the testing of the CEA software. As a result the testing demonstrated that it is possible to identify contributing factors with CEA validly. In addition, CEA received a very positive feedback from the experts. (orig.)

  8. Mesothelioma: profile of keratin proteins and carcinoembryonic antigen: an immunoperoxidase study of 20 cases and comparison with pulmonary adenocarcinomas.

    OpenAIRE

    Corson, J M; Pinkus, G. S.

    1982-01-01

    The distribution of keratin proteins and carcinoembryonic antigen (CEA) in 20 diffuse pleural malignant mesotheliomas and 20 adenocarcinomas of the lung was determined with the use of an indirect immunoperoxidase method. Keratin proteins were identified in all of the mesotheliomas, with strong staining observed in 17 of the cases. Tumor cells of various histologic types (tubular, papillary, solid, and spindle) revealed staining for keratin proteins. A variety of staining patterns were observe...

  9. How to Add Value to your Business with CEA: A Practical Approach

    Directory of Open Access Journals (Sweden)

    Daniel Cardenas

    2010-04-01

    Full Text Available Companies are always trying to differentiate themselves from the rest of the pack by applying different strategies such as improving customer service, increasing the efficiency of their operations, or reducing their costs. Most of the time, however, these goals are competing against each other for scarce resources, and managers often need to decide to concentrate on one. A small company can effectively and simultaneously accomplish these goals for a fraction of the cost by implementing communications-enabled business processes or solutions, which are a set of technology components that add real-time networking functionality to applications. One particular implementation of this framework is the one provided by Coral CEA. Coral CEA is a business ecosystem anchored around CEA functionalities that are offered as building blocks, out-of-the-box components that link the capabilities and intelligence of networks platforms with the power of current applications to provide a new set of features and functionalities. In this article, we show how a small company called Rezact, located in the ski resort town of Mont-Tremblant, Quebec, successfully implemented CEA capabilities within its own operations using Coral CEA services.

  10. The serum carbohydrate antigen 125 and carcinoembryonic antigen comparsion for the han and uyghur patients of terminal gallbladder cancer in xinjiang uygur autonomous region%新疆地区汉族与维吾尔族晚期胆囊癌患者血清糖类抗原125、癌胚抗原水平比较

    Institute of Scientific and Technical Information of China (English)

    毛拉艾沙·买买提; 薛峰; 依马木买买提江·阿不拉

    2013-01-01

    目的 比较新疆地区汉族与维吾尔族晚期胆囊癌患者血清糖类抗原125(CA125)、癌胚抗原(CEA)水平是否存在差异.方法 原发性晚期胆囊癌患者80例,其中维吾尔族40例,汉族40例;随机选取同期住院的胆囊炎患者60例作为对照组,其中维吾尔族34例,汉族26例;均进行血清CA125、CEA检测.结果 胆囊癌组的汉族、维吾尔族患者与对照组同民族患者CA125、CEA水平差异均有统计学意义(t=27.01、28.06、10.68、11.25,均P<0.05);而两组内汉族、维吾尔族患者间的CA125、CEA水平差异均无统计学意义(t=0.47、0.34、0.11、1.23,均P>0.05);胆囊癌Ⅳ期汉族、维吾尔族患者与Ⅴ期同民族患者CA125、CEA水平差异均有统计学意义(t=26.92、25.01、16.58、14.54,均P<0.05);汉族、维吾尔族的胆囊癌Ⅳ期患者之间、Ⅴ期患者之间的CA125、CEA水平差异均无统计学意义(t=1.13、0.11、0.38、0.07,均P>0.05).结论 新疆地区汉族与维吾尔族晚期胆囊癌患者血清CA125、CEA水平无差异,晚期胆囊癌患者进行CA125、CEA检测有助于病情诊断.%Objective To compare the differences on the serum carbohydrate antigen 125 and carcinoembryonic antigen for the Han and Uyghur patients of terminal gallbladder cancer in Xinjiang Uygur Autonomous Region.Methods 80 cases of patients with primary advanced gallbladder,including 40 cases of Uighur,Han 40 cases,The cholecystitis patients randomly selected from the same period of hospitalization of 60 cases as the control group.Including 34 cases of Uighur and Han 26 cases,Levels of serum CA125,CEA were detected.Results The gallbladder group of Han,Uygur patients and the control group with ethnic patients CA125,CEA level differences were statistically significant(t =27.01,28.06,10.68,11.25,all P < 0.05) ; CA125,CEA level differences between the two groups within the Han,Uygur patients were not statistically significant(t =0.47,0.34,0.1 l,1.23,all P > 0

  11. Detection and therapy of occult and metastatic medullary thyroid cancer with radiolabeled anti-carcino-embryonic-antigen antibodies and peptides

    International Nuclear Information System (INIS)

    Background: In many cases of medullary thyroid cancer (MTC), postsurgically elevated plasma calcitonin and/or CEA levels indicate persisting metastatic disease, although conventional diagnostic procedures (CT, MRI, invasive venous catheterization) fail to localize the responsible lesions. Patients with distant metastases have a poor prognosis and are left with few therapeutic choices. Recently, anti-CEA antibodies (MAbs) as well as somatostatin analogs (octreotide) have shown promising results in the staging or therapy of MTC. The aim of this abstract is to summarize our experience with these new approaches of diagnosis and treatment of MTC. Methods: At our department in Goettingen, a total of 26 patients with MTC was examined. 10 of them suffered of known, 14 of occult metastatic MTC, 2 patients were free of disease at the time of presentation. Results: 14 patients were investigated with monoclonal anti CEA MAbs labeled with 99mTc, 111In or 131 (receiving a total of 35 injections), 7 patients (6 with occult, 1 with known disease) were additionally studied with 111In-labeled octreotide. Two patients were treated so far with therapeutic doses of 131I-labeled anti-CEA MAbs. At CMMI, 18 patients with advanced MTC were treated with 131I-labeled anti-CEA MAbs (additional 8 patients receiving pure diagnostic studies with 131I-, 123I- or 99mTc-labeled MAbs). Conclusions: For the detection of occult MTC, anti-CEA MAbs and somatostatin analogs seem to have a sensitivity which is superior to conventional diagnostic modalities. Better detectability with anti-CEA antibodies (higher CEA expressions?), seems to be associated with more aggressive forms of MTC, whereas somatostatin receptor expression at normal CEA plasma levels may be associated with a more benign clinical course. This is in accordance to the study of Busnardo et al. (Cancer 1984;53:278-285) who showed elevated plasma CEA levels to be associated with a worse prognosis. Radio immunotherapy results with anti-CEA

  12. Technetium-99m labelling of the ior-CEA-1 monoclonal antibody: Evaluation of different methods

    International Nuclear Information System (INIS)

    In the present work, we evaluated the influence of experimental conditions on the characteristics of the radiolabelled ior-CEA-1 prepared by direct method, using 2-ME and stannous chloride as reductants and indirect method in which 2-IM is used to generate sulfhydryl groups in the protein. The following parameters were studied: number of free sulfhydryl groups of treated antibody, labelling efficiency, chemical and radiochemical composition of the radiolabelled antibodies as well as their immunological and biological properties. The properties of 99Tcm-ior-CEA-1 obtained by 2-ME method were compared to the ones of the 99Tcm-monoclonal antibody obtained from a commercial kit (BW 431/26). Some clinical studies using 99Tcm-ior-CEA-1 were also performed

  13. Chemistry of complexing molecules and environment. Report of the working group of the Cea ''mission environment''

    International Nuclear Information System (INIS)

    The Working group 'Chemistry of Complexing Molecules and Environment' of the Mission Environment (AG/ENV) identified themes for an original positioning of CEA on important issues of environmental research if a sufficiently strong demand appears. The research of CEA on the environment should be complementary to actions undertaken by other partners (official institutions, research organizations and industrial firms). The themes suggested are: the synthesis of new chelating molecules and new materials having specific properties, with the support of theoretical chemistry and modeling, analytical physical chemistry and speciation of species in relation to their eco-toxicity and their biogeochemical mobility in the natural environment. These themes, illustrated by examples of actions in progress at CEA or likely to be launched quickly, draw largely from recognized competences of the teams, generally developed for finalized nuclear applications: experimental, theoretical and instrumental competences. (author)

  14. Significance of CEA, CA15-3 and biochemical markers of bone turnover in the diagnosis of bone metastasis from breast cancer

    International Nuclear Information System (INIS)

    Objective: To evaluate the significance of tumor markers CEA and CA15-3, and biochemical markers of bone turnover (total procollagen type Ⅰ amino-terminal propeptide (TP Ⅰ NP), β-isomerized carboxyterminal propeptide (β-CTx), ALP and PTH) in the diagnosis of bone metastasis from breast cancer. Methods: A total of 78 patients (all females) with mean age (56.72 ± 10.76) years, who were diagnosed with breast cancer, were included in this study. The patients were divided into two groups based on radionuclide bone imaging: with bone metastasis (n=32) and without bone metastasis (n=46). The serum concentrations of CEA, CA15-3, TP Ⅰ NP, β-CTx, PTH, ALP were measured. Gleason scores were evaluated. The diagnostic value was evaluated by ROC curve.The two groups were compared using two-sample t test. The correlations between bone metastasis and tumor markers, bone metastasis and biochemical markers of bone turnover were analyzed with Pearson correlation and logistic analysis. Results: The serum levels of CEA, CA15-3, TP Ⅰ NP, β-CTx, PTH and ALP were significantly higher in the group with bone metastasis than those in the group without bone metastasis (t: 4.16-7.56, all P<0.05). For the diagnosis of bone metastasis from breast cancer, the AUC of CEA, CA15-3, TP Ⅰ NP, [β-CTx, PTH and ALP was 0.815, 0.887, 0.869, 0.852, 0.844, 0.731, respectively. Using the cut-off values of 4.18 μg/L for CEA, 0.04 U/L for CA15-3, 49.70 μg/L for TP Ⅰ NP, 0.47 pg/L for β-CTx,54.90 ng/L for PTH and 49.90 U/L for ALP, the sensitivities were 56.3% (18/32), 75.0% (24/32), 78.1% (25/32), 81.3% (26/32), 78.1% (25/32), 68.8% (22/32) and the specificities were 80.4% (37/46), 84.8% (39/46), 76.1% (35/46), 78.3% (36/46), 69.6% (32/46), 58.7% (27/46), respectively. CEA, CA15-3, TP Ⅰ NP, β-CTx, PTH, ALP and Gleason score were positively correlated with the presence of bone metastasis (r: 0.267-0.636, all P<0.05). CEA, CA15-3, TP Ⅰ NP, β-CTx, PTH and Gleason score were independent

  15. Effect of paclitaxel liposome combined with nedaplatin on serum HE4, CA125, CA19-9, AFP, CEA and T lymphocyte subsets in patients with advanced ovarian cancer

    Institute of Scientific and Technical Information of China (English)

    Su-Yu Zhu; Jie Tan; Chen-Lu Zhang; Qun-Ying Wu; Xue-Xin Xie; Hua-Fang Yin

    2016-01-01

    Objective:To study the effect of paclitaxel liposome combined with nedaplatin on serum HE4, CA125, CA19-9, AFP, CEA and T lymphocyte subsets in patients with advanced ovarian cancer.Methods:A total of 80 patients with advanced ovarian cancer in our hospital from December 2012 to December 2015 were enrolled in this study. The subjects were divided into control group (n=40) and experiment group (n=40) randomly. The control group were treated with paclitaxel and cisplatin, the experiment group were treated with paclitaxel liposome combined with nedaplatin. 21 days for a period of treatment and the two groups were treated for 3 periods. The serum HE4, CA125, CA19-9, AFP, CEA levels and peripheral blood CD3+, CD4+, CD8+ and NK cells of the two groups before and after treatment were compared. Results:There were no significantly differences of the serum HE4, CA125, CA19-9, AFP, CEA level and peripheral blood CD3+, CD4+, CD8+ and NK cells of the two groups before treatment (P>0.05). The serum HE4, CA125, CA19-9, AFP and CEA level of the two groups after treatment were significantly lower than before treatment (P<0.05), and that of experiment were significantly lower than control group (P<0.05). The peripheral blood CD3+, CD4+, CD8+and NK cells of the two groups after treatment were significantly lower than before treatment (P<0.05), and that of experiment were significantly higher than control group (P<0.05). Conclusions:Paclitaxel liposome combined with nedaplatin can significantly reduce the serum HE4, CA125, CA19-9, AFP and CEA levels, improve peripheral blood CD3+, CD4+, CD8+ and NK levels of patients with advanced ovarian cancer, and it was worthy clinical application.

  16. Overview of CEA research in the field of radionuclides migration; Syntheses des recherches menees par le CEA sur la migration des radionucleides

    Energy Technology Data Exchange (ETDEWEB)

    Poinssot, Ch.; Trotignon, L.; Tevissen, E

    2006-07-01

    This report presents a synthetic status of the researches conducted within the Nuclear Energy Division (CEA/DEN) in the field of radionuclides migration in three specific areas which have been chosen for their representativeness and potential impact: the migration of RN in PWR reactors, the migration of RN from a deep geological repository and the migration processes in the surface environments. In addition, some status is given about more generic research which is conducted in the field of RN speciation in the aqueous phase and at the interfaces and regarding chemistry / transport couplings. Additional information about the human and technical means involved in these fields of research in CEA/DEN is finally given in the Appendix. (authors)

  17. Effects of experimental immunosuppression in cattle with persistently high antibody levels to Salmonella Dublin lipopolysaccharide O-antigens

    OpenAIRE

    Nielsen Liza R; Jensen Asger L; Agerholm Jørgen S; Lomborg Sanne R

    2007-01-01

    Abstract Background Salmonella Dublin (S. Dublin) is a zoonotic bacterium which is host adapted to cattle. The bacterium can cause subclinical persistent infection in cattle (carriers), which may be reactivated. During reactivation, animals may shed bacteria, thus constituting a source of infection for other animals. Identification of such carriers is assumed to be critical in attempts to control and eradicate the infection. Some authors suggest that persistently high antibody levels in serum...

  18. Genome-wide association replicates the association of Duffy antigen receptor for chemokines (DARC) polymorphisms with serum monocyte chemoattractant protein-1 (MCP-1) levels in Hispanic children

    OpenAIRE

    Voruganti, V. Saroja.; Laston, Sandra; Haack, Karin; Mehta, Nitesh R.; Smith, C. Wayne; Cole, Shelley A.; Butte, Nancy F.; Comuzzie, Anthony G.

    2012-01-01

    Obesity is associated with a chronic low inflammatory state characterized by elevated levels of chemokines. Monocyte chemoattractant protein-1 (MCP-1) is a member of the cysteine-cysteine (CC) chemokine family and is increased in obesity. The purpose of this study was to identify loci regulating serum MCP-1 in obese Hispanic children from the Viva La Familia Study. A genome-wide association (GWA) analysis was performed in 815 children, ages 4-19 years, using genotypes assayed with the Illumin...

  19. African-American (AA) men with local-regional prostate cancer (PC) present with higher prostate specific antigen (PSA) levels than whites: results of RTOG 94-12

    International Nuclear Information System (INIS)

    Purpose/Objective: To use pretreatment serum PSA levels as an20), gleason score (2-5,6-7,7-10), race (whites and AAs), and two interactions viz (a) PSA by race (p=0.0012) and (b) PSA by total gleason score (p=0.0001). When race was replaced by educational status, or income, or both, the fits (0.8246,0.8197, and 0.7815, respectively) were not as good as the fit with race in the model. Conclusion: The findings of this nation-wide prospective registration study with a high percentage of AA patient participation confirms previous, smaller, geographically-limited studies (1,2,3) results that AA patients with non-metastatic PC present with a higher mean PSA values than whites. The multivariate findings imply that, for each level of total gleason score, there is a higher percentage of whites with PSA levels 20. Education and/or income as surrogates of sociological status could not completely explain the racial differences. Other reasons for health-care barriers among AAs need to be identified

  20. Report of transparency and nuclear safety 2007 CEA Fontenay aux Roses; Rapport transparence et securite nucleaire 2007 CEA Fontenay aux Roses

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Fontenay aux roses for the year 2007. After many years of decommissioning and dismantling of nuclear installations, the Center is now devoted (since 2005) to the development of research programmes on biology and biomedical technologies. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. (A.L.B.)

  1. Nuclear. In China, the CEA asserts itself as a team leader

    International Nuclear Information System (INIS)

    As the French government gave the CEA the responsibility of negotiating the nuclear French-Chinese nuclear strategic partnership, this article outlines the personality of the CEA's chairman, Bernard Bigot, but also the tensions which exist between the two main actors of the French nuclear industry, EDF and Areva, notably about their respective projects of development of a new reactor with China as far as EDF is concerned, and with Japan as far as Areva is concerned. In fact, China is asking France, not to build new reactors but to improve the performance and the safety in the existing ones

  2. Latest pixel size reduction of uncooled IR-FPA at CEA, LETI

    Science.gov (United States)

    Becker, Sebastien; Imperinetti, Pierre; Yon, Jean-Jacques; Ouvrier-Buffet, Jean-Louis; Goudon, Valérie; Hamelin, Antoine; Vialle, Claire; Arnaud, Agnès.

    2012-10-01

    Recent developments at the Infrared Lab (LIR) of CEA, LETI have been concentrated on the pixel size reduction of uncooled infrared detectors. With the support from French company ULIS, we have successfully demonstrated the technological integration of 12μm pixels on a commercial TV-format read-out circuit (VGA-ROIC) supplied by ULIS. The 12μm pixel has been designed, processed and characterized in CEA, LETI and first results showed exceptional performances. This paper presents the characterization and associated imagery results.

  3. The role of CA-242 and CEA in surveillance following curative resection for colorectal cancer.

    OpenAIRE

    Hall, N. R.; Finan, P. J.; Stephenson, B M; Purves, D. A.; Cooper, E H

    1994-01-01

    This study was undertaken to evaluate the role of a new tumour marker, CA-242, alone or in combination with CEA in the practical management of colorectal cancer patients after potentially curative resection. A cohort of 149 patients who had undergone 'curative' surgery was followed up according to an intensive protocol in order to detect recurrent disease. Over a median tumour marker follow-up period of 24 months there were 25 recurrences in 24 patients. Both CEA and CA-242 alone detected hal...

  4. The CEA and the alternative energies. Press tour 25 and 26 november 1999

    International Nuclear Information System (INIS)

    In the framework of the public information on the CEA center of Cadarache and Grenoble, a presentation of the researches concerning the alternative energies is proposed. The Cea is commissioned by the Public Authorities, to keep the nuclear option open and for the long-dated, to develop renewable energies. In this domain researches on fuel cells and photovoltaic solar energy are performed. The principle and the applications of the fuel cell and the photovoltaic are recalled to introduce the research programs and the partners. (A.L.B.)

  5. AMS/CEA Joint Aerial Campaign Nevada Test Site AMS Data Analysis Procedure

    International Nuclear Information System (INIS)

    The United States NNSA AMS (Aerial Measuring Systems) and the French Commissariat a l-Energie Atomique (CEA) participated in joint aerial survey activities during the period November 6-19, 2007. Survey activities were conducted near Las Vegas, Nevada, and at selected areas of the Nevada Test Site (NTS) under the auspices of the Accident Response Working Group (ARWG). The intent of the activities was to compare technologies, procedures, and data analysis techniques related to specific AMS and the CEA aerial radiation detection systems

  6. Changes in the legal status of the Commissariat a l'energie atomique (Cea)

    International Nuclear Information System (INIS)

    The Cea a public research institution was re-classified among the energy research establishments (11 december 2008). The Cea, as the result of exceptional circumstances and complex activities, is today entering into a new era and must confront many national and supranational challenges. It is preparing, in effect, for a future nuclear industry (safer, less polluting, non-proliferating and more economical) and is conducting research into other energy solutions within the framework of sustainable development and limited greenhouse gas effects (hydrogen technology, fusion, alternative energy technologies). It also has another goal, to make technological research available to industry, not only by developing micro and nano-technologies or even to contribute technology for the benefit of scientific knowledge of living creatures, matter, climate and the environment. The new classification of the Cea as a research establishment in the field of energy, a heading which includes three other entities (A.N.D.R.A., A.D.E.M.E. and I.F.P.), raises questions about its specialization and diversification. However, no substantive change was made to the drafting of the legislative provisions so it is possible to imagine that the Cea takes initiatives in other fields, at the instigation or with the approval of the government, a matter which is facilitated by its classification in the E.P.I.C. category (public establishment at industrial and commercial character). The Cea also represents France in international organisations in the nuclear sector, such as the OECD Nuclear energy agency (Nea), the International atomic energy agency (IAEA) and the European communities. It also participates in intergovernmental negotiations in the nuclear field and ensures any follow-up required with regard to any resulting agreements. today, the Cea plays a major role in the implementation of French nuclear policy with the aim of making available to countries wishing to develop nuclear energy, a framework

  7. Performances of Herschel/PACS bolometer arrays and future developments at CEA

    OpenAIRE

    Billot, N.; RODRIGUEZ, L.; Okumura, K; Sauvage, M.; Agnèse, P.

    2009-01-01

    The European Space Agency is building a space telescope to observe the Universe in the Far-IR and sub-millimeter regime of the electromagnetic spectrum. The scientific payload is composed of three instruments. One of them, PACS, is equipped with a novel type of bolometer arrays developed by CEA in the late 90's. We briefly present the PACS Photometer and the architecture of CEA filled bolometer arrays. We accessed the physics of the detectors and thoroughly measured their performances by deve...

  8. Assessment 2000 and regulation and method. Releases control and environmental survey of the CEA Centers; Bilan 2000 et reglementation et methode. Controle des rejets et surveillance de l'environnement des centres CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    The environment quality around the CEA centers is a major interest of its safety policy. These documents contribute to the public information on the radioactive liquid and gaseous releases of the CEA, according to the ministry authorization. The radioactivity monitoring activity, and the survey methods are also presented. Data analysis from 1996 to 2000, allows to follow the evolution. (A.L.B.)

  9. Overview of the long-lived radionuclide separation processes developed in connection with the CEA's spin programme

    International Nuclear Information System (INIS)

    One possible strategy for eliminating the long-term potential hazards associated with the storage of vitrified high level waste produced by the processing of irradiated nuclear fuels, is the transmutation of the long-life radionuclides in these wastes into short-life radionuclides by nuclear means, which requires the prior chemical separation of these long-life radionuclides from the nuclear waste mixture. To do this, the French CEA (Atomic Energy Commissionership) launched the SPIN (Separation-Incineration) research programme in 1991, under the law passed by the parliament on december 30, 1991. Research currently under way to develop separation processes for some long-life radionuclides concerns modifications of the Purex process, and the development of new solvent extraction processes. The research directions are briefly summarized here. (authors). 17 refs., 3 tabs

  10. Correlations between the CagA Antigen and Serum Levels of Anti-Helicobacter pylori IgG and IgA in Children.

    Science.gov (United States)

    Seo, Ji-Hyun; Lim, Chun Woo; Park, Ji Sook; Yeom, Jung Sook; Lim, Jae-Young; Jun, Jin-Su; Woo, Hyang-Ok; Youn, Hee-Shang; Baik, Seung-Chul; Lee, Woo-Kon; Cho, Myung-Je; Rhee, Kwang-Ho

    2016-03-01

    We tested correlations between anti-Helicobacter pylori IgG and IgA levels and the urease test, anti-CagA protein antibody, degree of gastritis, and age. In total, 509 children (0-15 years) were enrolled. Subjects were stratified as 0-4 years (n = 132), 5-9 years (n = 274), and 10-15 years (n = 103) and subjected to the urease test, histopathology, ELISA, and western blot using whole-cell lysates of H. pylori strain 51. The positivity rate in the urease test (P = 0.003), the degree of chronic gastritis (P = 0.021), and H. pylori infiltration (P pylori IgG was 732.5 IU/mL at 0-4 years, 689.0 IU/mL at 5-9 years, and 966.0 IU/mL at 10-15 years (P pylori IgA was 61.0 IU/mL at 0-4 years, 63.5 IU/mL at 5-9 years, and 75.0 IU/mL at 10-15 years (P pylori IgG and IgA titers increased with the urease test grade, chronic gastritis degree, active gastritis, and H. pylori infiltration. Presence of CagA-positivity is well correlated with a high urease test grade and high anti-H. pylori IgG/IgA levels. PMID:26955243

  11. Serum tetranectin is an independent prognostic marker in colorectal cancer and weakly correlated with plasma suPAR, plasma PAI-1 and serum CEA

    DEFF Research Database (Denmark)

    Høgdall, Claus K; Christensen, Ib J; Stephens, Ross W;

    2002-01-01

    to have an independent prognostic value for survival (log TN: HR = 0.47, 95% CI: 0.29-0.76); log soluble uPAR: HR = 1.65, 95% CI: 1.18-2.31; log CEA: HR = 1.I1, 95% CI: 1.03-1.20). Based on the multivariate model, a patient with a combination of low levels of TN and PAI-1 and elevated levels of...... prognostic factor in patients with colorectal cancer. TN may be valuable as a prognostic variable in future studies evaluating new treatment strategies for colorectal cancer....

  12. Elaboration of an alpha-numeric classification for file of matters of the documentation service of the CEA

    International Nuclear Information System (INIS)

    We give the principles of a classification of matters to square basis, suiting the needs of the Service, of Documentation of the C.E.A. We present the detail of the categories in the order of the 'columns', likewise the big scientific subdivisions at the CEA. (authors)

  13. A Label-Free Microelectrode Array Based on One-Step Synthesis of Chitosan–Multi-Walled Carbon Nanotube–Thionine for Ultrasensitive Detection of Carcinoembryonic Antigen

    Directory of Open Access Journals (Sweden)

    Huiren Xu

    2016-07-01

    Full Text Available Carcinoembryonic antigen (CEA has been an extensively used tumor marker responsible for clinical early diagnosis of cervical carcinomas, and pancreatic, colorectal, gastric and lung cancer. Combined with micro-electro mechanical system (MEMS technology, it is important to develop a novel immune microelectrode array (MEA not only for rapid analysis of serum samples, but also for cell detection in vitro and in vivo. In this work, we depict a simple approach to modify chitosan–multi-walled carbon nanotubes–thionine (CS–MWCNTs–THI hybrid film through one-step electrochemical deposition and the CS-MWCNTs-THI hybrid films are successfully employed to immobilize anti-CEA for fabricating simple, label-free, and highly sensitive electro-chemical immune MEAs. The detection principle of immune MEA was based on the fact that the increasing formation of the antigen-antibody immunocomplex resulted in the decreased response currents and the relationship between the current reductions with the corresponding CEA concentrations was directly proportional. Experimental results indicated that the label-free MEA had good selectivity and the limit of detection for CEA is 0.5 pg/mL signal to noise ratio (SNR = 3. A linear calibration plot for the detection of CEA was obtained in a wide concentration range from 1 pg/mL to 100 ng/mL (r = 0.996. This novel MEA has potential applications for detecting CEA for the research on cancer cells and cancer tissue slices as well as for effective early diagnosis.

  14. Clinical value of combined detection of serum CEA, CA15-3 and SF in lung cancer

    International Nuclear Information System (INIS)

    Objective: To investigate the clinical value of combined examination of serum CEA, CA15-3 and SF in lung cancer diagnosis. Methods: Serum concentrations of CEA, CA15-3 and SF were measured by chemiluminescent assay (CLA) in 99 patients with lung cancer and 25 patients with benign lung disease. Results: The positive rate of serum CEA, CA15-3 and SF in cases in lung cancer was 50.5%, 31.3% and 69.7%, respectively. Specificity of CEA, CA15-3 and SF measurements was 76%, 78% and 72%, respectively. In combined measurements, positive results of either two parameters revealed sensitivity and specificity of 82.8% and 92%, respectively. Conclusion: Combined measurements of CEA, CA15-3 and SF had evidently improved sensitivity and specificity in diagnosing lung cancer and thus were of good clinical value

  15. Solution recommended by the CEA to minimize fuel-cladding interaction problems

    International Nuclear Information System (INIS)

    To improve behavior of fuel elements under fast variation of power, the CEA developed a fracture resistant fuel: UO2 DCI and a cladding with low creep under irradiation: strong zircaloy 4. This paper gives main characteristics of these new materials and shows at what extent a better behavior of fuel pins is obtained concerning fuel-cladding interaction

  16. A study on the core analysis methodology for SMART CEA ejection accident-I

    International Nuclear Information System (INIS)

    A methodology to analyze the fuel enthalpy is developed based on MASTER that is a time dependent 3 dimensional core analysis code. Using the proposed methodology, SMART CEA ejection accident is analyzed. Moreover, radiation doses are estimated at the exclusion area boundary and low population zone to confirm the criteria for the accident. (Author). 31 refs., 13 tabs., 18 figs

  17. PZ Fiber Loss Measurements at LLNL and Plan to Confirm Results at CEA/Thomson

    CERN Document Server

    Bass, I L

    2000-01-01

    The objective of this paper is to confirm the slow-axis loss of less than 0.1 dB/m for the PZ fiber manufactured for LLNL by 3M and to provide samples of the fiber to CEA/Thomson that will permit them to readily verify this result.

  18. Experience gained at the CEA by thermal effluent utilization for agriculture and fish-breeding

    International Nuclear Information System (INIS)

    Results obtained at the CEA for waste heat utilization are examined. Water at T+100C is used for open air soil heating (forest, agriculture), eel breeding and greenhouse, heating (with the help of heat pumps). Water at T+230C or 300C is used for greenhouses. Facilities are described experience gained and results of the different exploitations are given

  19. Development of a kit lyophilized of Anti-CEA to be labeled with Tc-99m, radionuclide obtained by extraction with MEK, complemented with studies of stability

    International Nuclear Information System (INIS)

    supports 50 mCi of Tc-99m in a maximum volume of 5 mL. The percentage of fraction immunoreactive is 78,32 % (Ab-Ag). The percentage of transquelation or dissociation face to the L-cysteine is a 5,56 % for a molar relation MoAb: Cys 1:100 and 7,05 % for 1:1000, after 4 hours of incubation to 37oC. After 24 hours it is a 10, 45 % for 1:100 and 15, 75 % for 1:1000. The biological distribution in healthy mice sacrificed after four hours, demonstrates a percentage of dose of injection in blood 55,98 %, liver 14,90 %; kidneys 3,8 %; intestines 18,07 %; in stomach and thyroid gland he is a minor of 1 %. These experimental lots of lyophilized kit present a stability of 13 months with radiochemical purity more than 93 %; and 99mTc-Anti-CEA it has a stability of up to 4 hours, post-labelling, with radiochemical purity more than 90 %. With these results, shows us that we have developed to a stable lyophilized kit of Anti-CEA to be labelled by Tc-99m (MEK), obtaining 99mTc-Anti-CEA with a high radiochemical purity more than 97 %, values acceptable and comparable with the published reports with Tc-99m, coming from a generator of adsorption. The immunoreactivity test, challenging with L-cysteine, biological distribution and study of stability have demonstrated the high quality of this lyophilized kit that keeps the capacity of biological recognition face its antigen CEA. (author)

  20. The study of special killing effect of CD3AK on anti-CEA-positive tumor enhanced by DC loaded with CEA-rV%荷CEA-rV的DC增强CD3AK对CEA阳性肿瘤特异性杀伤作用的研究

    Institute of Scientific and Technical Information of China (English)

    祁岩超; 王新帅; 扬波

    2006-01-01

    Objective: To survey the special killing activity of CD3AK on anti-CEA-positive tumor enhanced by umbilical cord blood dendritic cell (DC) loaded with CEA recombinant vaccinia virus (CEA-rV). Methods: Freshly isolated umbilical blood mononuclear cells (UBMC) were cultivated for 3 h. Suspension cells and attached cells were used to induce CD3AK cells and DC separately. DC was loaded with CEA-rV on the 3rd day to prepare CEA-rV+DC. CD3AK cells were co-cultured with CEA-rV+DC on the 8th day, to prepare CEA-rV+DC+CD3AK. The killing activity of each effector's cell, which included UBMC, CD3AK, DC+CD3AK and CEA-rV+DC+CD3AK, was measured respectively by MTT reduction assay. Results: (1) 4 target cells were confirmed by CEA monoclonal antibody of rabbit anti-human. Lovo and A549 were really CEA positive cell lines, while Bel-7402 and K562 were CEA negative cell lines. (2) It was showed by flow-cytometry that the mature DC cultured at 10th day expressed MHCⅠ, Ⅱ molecules such as CD86, CD80, CD83 and CD40 highly, but CD123 lowly. The expression rates of CD86, CD80, CD83 and CD40 was 82.7%, 51.1%, 57.5% and 69.4%, respectively. The appearances and intra-cellular structures of DC were observed through light and electron microscope. The diameter of mature DC was 15-20 μm presented the irregular morphologic appearance, much prominences and pseudopodium. There were abundant mitochondria and endoplasmic reticulum in DC endochylema. (3) The rates of CD3, CD4, CD8 and CD28 in CD3AK cells group were 2 folds higher than that in UBMC group by FACS.It was said that the numbers of the mature T lymphocyte in CD3AK cells group were much greater than that in UBMC group. (4)The killing activities to 4 target cells of 3 effector's cells, which included CEA-rV+DC+CD3AK, DC+CD3AK and CD3AK, were much greater than that of UBMC (P<0.01). Moreover, comparing with the killing activities of 4 effector's: CEA-rV+DC+CD3AK group > DC+CD3AK group > CD3AK group > UBMC group. It showed that

  1. Ultrasensitive electrochemical immunoassay for carcinoembryonic antigen based on three-dimensional macroporous gold nanoparticles/graphene composite platform and multienzyme functionalized nanoporous silver label

    International Nuclear Information System (INIS)

    Graphical abstract: Three-dimensional macroporous AuNPs/graphene complex (3D-AuNPs/GN) and functionalized NPS were prepared to immobilize Ab1 and Ab2 respectively and combined to fabricate a sandwich-type ultrasensitive electro-chemical immunosensor for detecting CEA. -- Highlights: •Ultrasensitive electrochemical immunoassay for detecting CEA was developed. •3D-AuNPs/GN was employed as the carrier of primary antibodies. •Multienzyme functionalized nanoporous silver was used as signal enhancer. -- Abstract: Three-dimensional macroporous gold nanoparticles/graphene composites (3D-AuNPs/GN) were synthesized through a simple two-step process, and were used to modify working electrode sensing platform, based on which a facile electrochemical immunoassay for sensitive detection of carcinoembryonic antigen (CEA) in human serum was developed. In the proposed 3D-AuNPs/GN, AuNPs were distributed not just on the surface, but also on the inside of graphene. And this distribution property increased the area of sensing surface, resulting in capturing more primary antibodies as well as improving the electronic transmission rate. In the presence of CEA, a sandwich-type immune composite was formed on the sensing platform, and the horseradish peroxidase-labeled anti-CEA antibody (HRP-Ab2)/thionine/nanoporous silver (HRP-Ab2/TH/NPS) signal label was captured. Under optimal conditions, the electrochemical immunosensor exhibited excellent analytical performance: the detection range of CEA is from 0.001 to 10 ng mL−1 with low detection limit of 0.35 pg mL−1 and low limit of quantitation (LOQ) of 0.85 pg mL−1. The electrochemical immunosensor showed good precision, acceptable stability and reproducibility, and could be used for the detection of CEA in real samples. The proposed method provides a promising platform of clinical immunoassay for other biomolecules

  2. Detection of filarial antigen in urine of humans with Wuchereria bancrofti infection by immunoradiometric assay

    International Nuclear Information System (INIS)

    Human urine samples of different groups were analyzed for the presence of filarial antigen by immuno-radiometric assay (IRMA) using 125I-Rabbit IgG antibodies to B. malayi antigen. Six out of ten microfilaraemia, one out of five clinical filariasis had detectable antigen, while none of the endemic and non-endemic normals (n=12) were positive. The effect of heat-acid treatment on the detectability of antigen in the urine and serum was explored. Heat-acid treatment in general did not reduce the level of filarial antigen in the urine samples while there was a measurable reduction in the antigen levels in the sera. (author)

  3. Free cancer cell detection in peritoneal cavity in gastric cancer patients by RT-PCR for CEA

    International Nuclear Information System (INIS)

    Authors applied RT-PCR assay to detecting CEA expressing free cancer cells in peritoneal cavity of 114 gastric cancer patients to find an indication for prophylactic treatment to prevent peritoneal recurrence. Sixty-three of 114 cases were positive for RT-PCR, of which 16 cases were positive for cytologic examination and 47 cases were negative. Forty-nine of 51 cases who were negative for RT-PCR were negative for cytologic examination. Positivity for RT-PCR according to the depth of invasion were as follows : two (28.6 %) of seven cases whose cancer invaded mucosal or submucosal layer were positive. Ten (45.5 %) of 22 cases whose cancer invaded muscular or subserosal layer were positive. Forty-one (57.7 %) of 71 serosa involved cases were positive. Eleven (78.6 %) of cases who had grossly perioneal seedings were positive (p=0.026). However, all of 7 EGC cases, 19 of 22 cases whose cancer invaded to muscle layer or to subserosa were negative for cytologic examination, and eight of 13 cases who had had peritoneal seedings were positive. Positivity for RT-PCR according to cell differentiation were as follows: forty-two (61.8 %) of 68 cases who cancer were poorly differentiated type were positive. (p=0.163) Serum level of CEA of RT-PCR positive group and that of negative group were not statistically different. It was revealed that RT-PCR was more sensitive than cytologic examination in detecting free tumor cells, especially in pm, ss and serosa positive cancers, so if further study with more cases and longer follow-up is performed, its role as prognostic factor and an indication of prophylactic therapy will be clarified. (author). 22 refs., 5 tabs

  4. Au-F127 strawberry-like nanospheres as an electrochemical interface for sensitive detection of carcinoembryonic antigen in real sample.

    Science.gov (United States)

    Li, Juan; Xie, Hangqing; Liu, Yuhong; Ren, Hang; Zhao, Wenbo; Huang, Xiaohua

    2015-11-01

    Nanomaterial-based signal-amplification strategies hold a great promise in realizing sensitive biological detection. A simple label-free electrochemical immunosensor for sensitive detection of carcinoembryonic antigen (CEA) was developed by immobilizing anti-CEA antibodies onto the Au-F127 strawberry-like nanospheres modified glassy carbon electrode (Au-F127/GCE). The Au-F127 strawberry-like nanospheres offered a large surface and multifunctional substrate for the effective immobilization of anti-CEA and the existence of Au could accelerate electron transfer and make the electrochemical signal amplified. The Au-F127 nanocomposites and anti-CEA were characterized by transmission electron microscopy (TEM), polycrystalline electron diffraction ring pattern, ultra-violet visible (UV-vis) spectra and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were employed to verify the stepwise assembly of the immunosensor and evaluated the analytical performance of the fabricated immunosensor, respectively. The immunosensor showed a wide liner response range between 0.01 and 80 ng mL(-1) with a low detection limit of 0.24 pg mL(-1) at a signal-to-noise (S/N) ratio of 3. Additionally, the proposed method was successfully applied to determine CEA in human serum samples with satisfactory results. PMID:26452840

  5. Cancer vaccine--Antigenics.

    Science.gov (United States)

    2002-01-01

    Antigenics is developing a therapeutic cancer vaccine based on heat-shock proteins (HSPs). The vaccine [HSPPC-96, Oncophage] is in a pivotal phase III clinical trial for renal cancer at 80 clinical sites worldwide. The trial is enrolling at least 500 patients who are randomised to receive surgical removal of the primary tumour followed by out-patient treatment with Oncophage((R)) or surgery only. This study was initiated on the basis of results from a pilot phase I/II study and preliminary results from a phase II study in patients with renal cell cancer. In October 2001, Oncophage was designated as a fast-track product by the Food and Drug Administration in the US for the treatment of renal cell carcinoma. Oncophage is in phase I/II trials in Italy for colorectal cancer (30 patients) and melanoma. The trials in Italy are being conducted at the Istituto dei Tumouri, Milan (in association with Sigma-Tau). Preliminary data from the phase II trial for melanoma was presented at the AACR-NCI-EORTC International Conference in Florida, USA, in October 2001. Oncophage is also in a phase I/II (42 patients) and a phase II trial (84 patients) in the US for renal cell cancer, a phase II trial in the US for non-Hodgkin's lymphoma (35 patients), a phase II trial in the US for sarcoma (20-35 patients), a phase I/II trial in the US for melanoma (36 patients), and phase I/II trials in Germany for gastric (30 patients) and pancreatic cancers. A pilot phase I trial in patients with pancreatic cancer began in the US in 1997 with 5 patients enrolled. In November 2000, Antigenics announced that this trial had been expanded to a phase I/II study which would now include survival as an endpoint and would enroll 5 additional patients. The US trials are being performed at Memorial Sloan-Kettering Cancer Center and the M.D. Anderson Cancer Center. The trials in Germany are being carried out at Johannes Gutenberg-University Hospital, Mainz. Oncophage is an autologous vaccine consisting of

  6. Diagnostic value of Cyfra21-1, SCC and CEA for differentiation of early-stage NSCLC from benign lung disease

    OpenAIRE

    Chen, Feng; Wang, Xiu-Ying; Han, Xiao-Hong; Wang, Hai; Qi, Jun

    2015-01-01

    Non-small cell lung cancer (NSCLC), which account for the most of lung carcinoma, is sometimes difficult to differentiate from benign lung diseases presented with nodular shadow in imaging scan. There is a need to find another non-invasive way to diagnosis early-stage NSCLC. To examine the potential diagnostic value of SCC, CFYRA 21-1 and CEA for the differentiation of early-stage NCSCL from benign lung diseases, we analyzed serum levels of tumor markers in 278 patients, including 248 patient...

  7. Preoperative CYFRA 21-1 and CEA as Prognostic Factors in Patients with Stage I Non-Small Cell Lung Cancer

    OpenAIRE

    Blankenburg, Florian; Hatz, Rudolf; Nagel, Dorothea; Ankerst, Donna Pauler; Reinmiedl, Judith; Gruber, Christine; Seidel, Dietrich; Stieber, Petra

    2008-01-01

    Objective: To validate the prognostic value of preoperative levels of CYFRA 21-1, CEA and the corresponding tumor marker index (TMI) in patients with stage I non-small cell lung cancer (NSCLC). Methods: Two hundred forty stage I NSCLC patients (80 in pT1 and 160 in pT2; 100 squamous cell carcinomas, 91 adenocarcinomas, 32 large-cell carcinomas, 17 with other histologies; 171 males and 69 females) who had complete resection (R0) between 1986 and 2004 were included in the analysis. CYFRA 21-1 a...

  8. Situation in 2002. Radiation protection at the Cea

    International Nuclear Information System (INIS)

    This work has for objective to give a situation calculated from some work indicators made in 2002 and to make obvious the improvement approach that was made during these last years. To continue this progress approach implies a collective will through the different operating levels, to take into account the radiological risk at the projects conception and till the implementation of operations in nuclear facilities, on including the protection personnel but also the public protection and environment protection; the mobilization of human and technique resources to control the risks and make the continuous surveillance in installation, the professionalism of each actor and to keep at high level their competence. (N.C.)

  9. Project: Decommissioning and Reuse of Licensed Nuclear Facilities at the CEA Centre, Fontenay-aux-Roses, (CEA-FAR) France. Annex A.I-3

    International Nuclear Information System (INIS)

    CEA-FAR is the CEA's oldest facility and is situated in the southern suburbs of Paris. It was opened on 26 March 1946 to host the first French atomic pile, which was named ZOE, and first went critical on 12 December 1946. The first laboratories were installed in existing buildings on the site. In the early 1950s, a number of nuclear facilities to be used for R and D work were built within the perimeter of the centre. This included the construction and operation of a pilot plant facility for the reprocessing of spent nuclear fuel. As space was restricted, all these installations were decommissioned and demolished to enable the construction of more modern second generation facilities. The Pu Pilot Plant for irradiated fuel reprocessing (the prototype of the Marcoule UP1 Plant) was used from 1953 to 1958. The dismantling of the equipment from the Pu Pilot Plant, the demolition of the buildings and the removal of the grounds to constitute a uniform platform were completed at the end of the 1950s. This operation made it possible to release the grounds on which building 18 was built, and also buildings 91 and 52. These buildings were constructed during the 1960s within the framework of the development of the CEN-FAR programmes. In the late 1950s and early 1960s, in addition to ZOE (NLF 11), which remained in service, the following second generation facilities were built: radio-metallurgy installations (NLF 58 and 59), radiochemistry and chemical engineering installations for the reprocessing of spent fuel (NLF 57), and installations for the treatment and interim storage of solid and liquid waste (NLF 34 and 73), which were required for pursuing the research programmes. These latter buildings are currently being cleaned up and dismantled. ZOE was closed down and partly dismantled in the 1970s to former IAEA Stage 2 and the research areas around the pile were transformed into a museum. The facilities shut down and dismantled in the 1980s were plutonium metallurgy

  10. Progress of the BT-EdF-CEA project. The lithium polymer battery; Avancees du projet BT-EdF-CEA. Batterie lithium polymere

    Energy Technology Data Exchange (ETDEWEB)

    Marginedes, D.; Majastre, H. [Bollore Technologies, 29 - Quimper (France); Baudry, P.; Lascaud, S. [Electricite de France, 77 - Moret sur Loing (France). Direction des Etudes et Recherches; Bloch, D.; Lebrun, N. [CEA Grenoble, CEREM, 38 (France)

    1996-12-31

    The lithium-polymer energy storage technology requires the production of thin films of huge surface. The BT-EdF-CEA consortium has studied the various manufacturing techniques of these films and their assembly. The process was chosen according to its productivity, low expensiveness, ecological impact and energy performances with capacities reaching 40 Ah. This paper explains: the objectives and specifications of the project, the advantage of the consortium and the role of the different partners, the results (coating, dry extrusion and battery element manufacturing techniques), and the electrochemical performances of the elements. (J.S.)

  11. Producing energy without greenhouse effect gases: the CEA action; Produire de l'energie sans gaz a effet de serre: l'action du CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    Major actor in the domain of new energy technologies, the CEA manages the french research on the hydrogen and the fuel cells. It is also implied with INES (National Institute for the Solar Energy) in the photovoltaic and thermal solar. With the IFP (French Petroleum Institute), it manages research on biofuels. Of course the thermonuclear fusion, for the development of the energy of the future, is in its research program too. This information document presents the possibilities of these energies and the associated research programs. (A.L.B.)

  12. The evaluation of the nuclear facilities safety at the CEA from 1999 to 2001; Le bilan de la surete des installations nucleaires du CEA du 1999 a 2001

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-11-01

    The aim of this document is the presentation of an evaluation of the problems and the safety methods in the concerned period. The first chapter presents the nuclear safety in the CEA. The second chapter is devoted to the organization and the quality for the safety: liabilities, audits, relations with the safety authorities and with the public. The chapters three and four deal respectively with the methodological and technical abilities supporting the exploitation teams and with the nuclear safety projects. The last chapter presents the experiments and events from 1999 to 2001. (A.L.B.)

  13. Continuously tritium monitoring of the pipe of liquid effluents at the Cea Cadarache; Controle en continu du tritium de la conduite des effluents liquides du CEA Cadarache

    Energy Technology Data Exchange (ETDEWEB)

    Pira, Y

    2004-07-01

    This report is oriented toward the radiation protection of environment that is an essential component of radiation protection. It is necessary to detect any solid, liquid or gaseous abnormal release and to find its origin. The present study bears on a detection instrument in continuously to find tritium in liquid effluents of the Cea Cadarache. After having study the functioning principle of this device, an evaluation of its performances has been realised ( back noise, yield, detection limit) and to a checking in real conditions of utilization. (N.C.)

  14. Methods for examination of antigenicity of heterogeneous polymerized hemoglobin

    International Nuclear Information System (INIS)

    Objective: To choose and establish the methods for examination of heterogeneous polymerized hemoglobin in order to offer the reference for evaluating the antigenicity of heterogeneous polymerized hemoglobin against human. Methods: Antigenicity of heterogeneous polymerized hemoglobin was examined for hypersensitivity, cell-mediated immunity reaction, humoral immunity reaction and cross-reaction of antigen. Results: The rabbit and guinea pig did not give rise to hypersensitivity. In immunized rabbits, the level of serum total IgG was normal, but the level of serum specific IgG was high. The examination of B lymphocytes showed that there was no significant difference (P>0.05) in comparison with control. Cross-reaction of antigen proved that bovine hemoglobin had cross-reaction with human hemoglobin. Suggesting that they may be homologous, the level of the serum specific antibody is high in the immunized animal. According to the immunology theories, the polymerized hemoglobin has antigenicity. (authors)

  15. Antigen smuggling in tuberculosis.

    Science.gov (United States)

    Hudrisier, Denis; Neyrolles, Olivier

    2014-06-11

    The importance of CD4 T lymphocytes in immunity to M. tuberculosis is well established; however, how dendritic cells activate T cells in vivo remains obscure. In this issue of Cell Host & Microbe, Srivastava and Ernst (2014) report a mechanism of antigen transfer for efficient activation of antimycobacterial T cells. PMID:24922567

  16. Antigen detection systems

    Science.gov (United States)

    Infectious agents or their constituent parts (antigens or nucleic acids) can be detected in fresh, frozen, or fixed tissues or other specimens, using a variety of direct or indirect assays. The assays can be modified to yield the greatest sensitivity and specificity but in most cases a particular m...

  17. Diagnostic utility of serum and pleural fluid carcinoembryonic antigen, and cytokeratin 19 fragments in patients with effusion from nonsmall cell lung cancer

    OpenAIRE

    Sushil Kumar Sharma; Sanjay Bhat; Vikas Chandel; Mayank Sharma; Pulkit Sharma; Sakul Gupta; Sashank Sharma; Aijaz Ahmed Bhat

    2015-01-01

    Aims: To assess the diagnostic value of CEA and CYFRA 21-1 (cytokeratin 19 fragments) in serum and pleural fluid in non small cell lung cancer with malignant pleural effusion (MPE). Settings and Design: Two subsets of patients were recruited with lymphocytic exudative effusion, one subset constituted diagnosed patients of NSCLC with malignant pleural effusion and the other subset of constituted with Tubercular pleural effusion. Methods and Material : CYFRA 21-1 and CEA levels were measured us...

  18. Aspergillus antigen skin test (image)

    Science.gov (United States)

    The aspergillus antigen skin test determines whether or not a person has been exposed to the mold aspergillus. It is performed by injecting an aspergillus antigen under the skin with a needle. After 48 ...

  19. Stool Test: H. Pylori Antigen

    Science.gov (United States)

    ... All About Food Allergies Stool Test: H. Pylori Antigen KidsHealth > For Parents > Stool Test: H. Pylori Antigen Print A A A Text Size What's in ... sample is used to determine if H. pylori antigens are present in your child's gastrointestinal (GI) system. ...

  20. Fusion technology. Annual report of the Association CEA/EURATOM 1997

    Energy Technology Data Exchange (ETDEWEB)

    Magaud, P.; Le Vagueres, F

    1998-12-31

    The research and development work performed by the French EURATOM-CEA Association for fusion technology is part of the Fusion Programme of the European Community. This report compiles the work carried out during the year 1997 as follows: The ITER CEA activities and related developments are described in the first section (plasma facing components, vacuum vessel and shield, magnets, remote handling, safety); The second part is dedicated to the Long Term activities as Blankets and material developments, long term safety, socio-economic problem; The Underlying Technology activities are compiled in the third part of this report (plasma facing components, vacuum vessel and shield, magnets, remote handling, safety); And the fourth part describes the inertial confinement studies. (K.A.)

  1. Diatomácea marinha Coscinodiscus wailesii Gran et Angst(BACILLARIOPHYCEAE)

    OpenAIRE

    Ascencio, Poliana G. Marson

    2012-01-01

    A diatomácea marinha cêntrica Coscinodiscus wailesii Gran et Angst, considerada uma espécie nociva, vem despertando grande interesse ecológico. Suas florações, vêm prejudicando áreas de pesca e cultivo em diferentes regiões do mundo. Entretanto essa microalga marinha é muito pouco estudada em relação ao seu crescimento celular, composição química, produção e caracterização de polissacarídeos e ácidos graxos. Sendo assim, nesse trabalho foi realizado o isolamento e o cultivo da diatomácea C. w...

  2. The French 'CEA 86' multigroup cross-section library and its integral qualification

    International Nuclear Information System (INIS)

    This paper describe the up-dated 99 groups library of the APOLLO French neutron computer code, the denominated 'CEA 86' library. The multigroup cross-section sets are based on the more recent nuclear data evaluations. The THEMIS code was generally used for the JEF-1 processing. In order to account for recent differential measurements and to improve the consistency between calculation and integral experiments, we produced our own CEA evaluations for the actinide nuclides: 235U, 238U, 239Pu, 240Pu, 241Am. This new APOLLO library was checked against critical experiments and PWR measurements: computed Conversion Factor, Reactivity Coefficients, Multiplication Factor, and Pu build-up are now in good agreement with LWR experimental results. PWR Pu recycling calculations, as does as HCLWR design studies, are also significantly improved. (author)

  3. 2004 annual report. Defense, safety, energy, information, health. CEA in the center of big European challenges

    International Nuclear Information System (INIS)

    This document is the 2004 annual report of the French atomic energy commission (CEA). It presents the R and D activities of the CEA in three main domains: 1 - defense and safety, maintaining perenniality of nuclear dissuasion and nuclear safety: supplying nuclear weapons to armies, maintaining dissuasion capability with the simulation program, sharing R and D means with the scientific community and the industrial world, designing and maintaining naval nuclear propulsion reactors, cleansing Marcoule and Pierrelatte facilities, monitoring treaties and fighting against proliferation and terrorism; 2 - energy, developing more competitive and cleaner energy sources: nuclear waste management, optimization of industrial nuclear activities, future nuclear systems and new energy technologies, basic research on energy, radiobiology and toxicology; 3 - information and health, valorizing industry thanks to technological research and supplying new tools for health and medical research: micro- and nano-technologies, software technologies, basic research for industrial innovation, nuclear technologies for health and bio-technologies. (J.S.)

  4. Cancer mortality of nuclear workers of CEA and COGEMA from 1969 to 1986

    International Nuclear Information System (INIS)

    Cancer mortality of the nuclear workers of CEA and COGEMA has been collected by the occupational health services of both firms from 1969 to 1986. The data are related only to the workers who died when in activity. Only very few workers left CEA and COGEMA before retirement so we consider this mortality survey as describing correctly the cancer mortality for the age groups less than 60-65 years old. Compared to the national mortality of same sex, age and calendar period, by the method of indirect standardization, the only excess observed was in the female population, linked to breast cancer mortality. The male population demonstrated a high healthy worker effect, even for cancer mortality. This study has now to be completed by an typical epidemiological cohort study in order to test cancer mortality after retirement and to discuss a possible relation with occupational exposure. (author)

  5. The role of the CEA in the first steps of neutronics

    International Nuclear Information System (INIS)

    A historical review of the early developments of neutronics and nuclear energy experimentations in France since World War II, with the creation of the CEA in 1945: neutronics theory investigations through the works of some leading french physicists, giving rise to the calculations of the first French heavy water reactor Zoe and its numerous experimentations, the theoretical works on mathematical physics in the fifties and sixties: spherical harmonics decompositions, double spherical harmonics method, calculations of the effect of cavities and cooling ducts on diffusion coefficient and reactivity, the development of models for phenomenon description. The various ways of knowledge and information dissemination involving the CEA at that time are presented, leading to the creation in 1956 of the INSTN nuclear techniques and sciences institute

  6. Technetium-99m labelling of the IOR CEA 1 monoclonal antibody: evaluation of different methods

    Energy Technology Data Exchange (ETDEWEB)

    Gano, L.; Fernandes, C.; Patricio, L. [Inst. Tecnologico Nuclear, Sacavem (Portugal); Cantinho, G.; Santos, A.I.; Pena, H. [F.M.L., Lisboa (Portugal). Inst. Medicina Nuclear; Vieira, R.; Salgado, L. [IPOFG, Lisboa (Portugal). Servico Medicina Nuclear

    1997-09-01

    Aim: The aim of this study was to investigate the in vivo and in vitro properties of {sup 99m}Tc labelled monoclonal antibody, IOR CEA 1 when radiolabelled by different methods. Methods: To achieve that purpose IOR CEA was directly radiolabelled via 2-mercaptoethanol (2-Me) and stannous ion (SnCl{sub 2}) reduction and indirectly via the 2-iminothiolane (2-Im) conjugation. The resulting {sup 99m}Tc-MoAbs were analysed for number of free sulfhydryl groups, chemical and radiochemical purity (checked by HPLC and SDS PAGE), immunoreactivity and biological distribution in mice. Results: Experimental results indicated a similar radiochemical purity and immunoreactivity for direct labelling methods and a decrease of both for 2-Im method. 2-Me antibody reduction led to a high antibody fragmentation as indicated by non-denaturing SDS PAGE analysis. Nevertheless SnCl{sub 2} and 2-Im labels revealed lower in vivo stability. Conclusion: {sup 99m}Tc-(2-Me) IOR CEA presented favourable in vitro and in vivo properties. Therefore this label was compared to {sup 99m}Tc-monoclonal antibody BW 431/26. Similar characteristics were found. Clinical studies also revealed identical biodistribution profile. (orig.) [Deutsch] Ziel: Ziel der vorliegenden Studie war die Untersuchung der in vitro und in vivo Eigenschaften Technetium-markierter monoklonaler Antikoerper. Methoden: Hierzu wurde IOR CEA 1 entweder direkt, nach Reduktion mit 2-Mercaptoethanol (2-Me) bzw. Zinn(II)chlorid (SnCl{sub 2}), oder indirekt nach Konjugation mit 2-Iminothiolan (2-Im) markiert. Die Integritaet des reduzierten bzw. konjugierten Antikoerpers wurde mittels Bindungsassays und Elektrophorese ermittelt. Die radiochemischen Ausbeuten bzw. Reinheiten wurden chromatographisch bestimmt. Die pharmakokinetischen Eigenschaften von {sup 99m}Tc-IOR CEA 1 wurden tierexperimentell im Vergleich zu {sup 99m}Tc-BW 431/26 ermittelt. In einer ersten Studie wurden {sup 99m}Tc-(2-Me-)IOR CEA 1 und {sup 99m}Tc-BW 431/26 verglichen

  7. Corium behaviour research at CEA Cadarache. The PLINIUS prototypic corium experimental platform

    International Nuclear Information System (INIS)

    In the hypothetical case of a Light Water Reactor severe accident, the reactor core could melt and form with structure materials, and eventually concrete, a complex mixture called corium. The knowledge of corium behaviour is one of the key to increase the safety of the nuclear reactors. In the framework of severe accident studies, CEA has undertaken for many years a large RandD program devoted to this topic. At CEA-Cadarache, a specific platform, called PLINIUS, has been developed and is dedicated to studies of prototypic corium (i.e. with uranium dioxide). In this paper, the PLINIUS platform four facilities and the experiments on corium behaviour are described.(author)

  8. Fusion technology. Annual report of the Association CEA/EURATOM 1997

    International Nuclear Information System (INIS)

    The research and development work performed by the French EURATOM-CEA Association for fusion technology is part of the Fusion Programme of the European Community. This report compiles the work carried out during the year 1997 as follows: The ITER CEA activities and related developments are described in the first section (plasma facing components, vacuum vessel and shield, magnets, remote handling, safety); The second part is dedicated to the Long Term activities as Blankets and material developments, long term safety, socio-economic problem; The Underlying Technology activities are compiled in the third part of this report (plasma facing components, vacuum vessel and shield, magnets, remote handling, safety); And the fourth part describes the inertial confinement studies. (K.A.)

  9. Increased serum levels of tumour-associated trypsin inhibitor independently predict a poor prognosis in colorectal cancer patients

    International Nuclear Information System (INIS)

    There is an insufficient number of reliable prognostic and response predictive biomarkers in colorectal cancer (CRC) management. In a previous study, we found that high tumour tissue expression of tumour-associated trypsin inhibitor (TATI) correlated with liver metastasis and an impaired prognosis in CRC. The aim of this study was to investigate the prognostic validity of serum TATI (s-TATI) in CRC. We further assessed the prognostic value of carcino-embryonic antigen in serum (s-CEA) and the interrelationship between s-TATI and TATI in tissue (t-TATI). Using an immunofluorometric assay, s-TATI levels were analysed in 334 preoperatively collected serum samples from patients with CRC. Spearman's Rho and Chi-square test were used for analysis of correlations between s-TATI and clinicopathological parameters, s-CEA and t-TATI. Kaplan-Meier analysis and Cox uni- and multivariate regression analysis were used to estimate disease free survival (DFS) and overall survival (OS) according to quartiles of s-TATI and cut-offs derived from ROC-analysis of s-TATI and s-CEA. Increased levels of s-TATI were associated with a reduced DFS (HR = 2.00; 95% CI 1.40-2.84, P < 0.001) and OS (HR = 2.40; 95% CI 1.74-3.33, P < 0.001). (HR = 2.89; 95% CI 1.96-4.25). This association remained significant in multivariate analysis. The association for OS remained significant in multivariate analysis (HR = 1.51; 95% CI 1.03-2.22, P = 0.034 for DFS and HR = 1.78; 95% CI 1.25-2.53, P = 0.001 for OS). There was no significant association between s-TATI and t-TATI. The prognostic value of s-CEA was also evident, but somewhat weaker than for s-TATI. High preoperative s-TATI levels predict a poor prognosis in patients with CRC, and the prognostic value is independent of established prognostic parameters and t-TATI expression. These data suggest that s-TATI might be a useful marker for prognostic stratification in CRC

  10. Report of transparency and nuclear safety 2007 CEA Fontenay aux Roses

    International Nuclear Information System (INIS)

    This report presents the activities of the CEA Center of Fontenay aux roses for the year 2007. After many years of decommissioning and dismantling of nuclear installations, the Center is now devoted (since 2005) to the development of research programmes on biology and biomedical technologies. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. (A.L.B.)

  11. EURATOM-CEA association contributions to the 21st symposium on fusion technology

    International Nuclear Information System (INIS)

    The 27 contributions of EURATOM-Cea association have been gathered with 6 additional papers and 1 invited paper in this document. Most papers concern Tore-Supra and deal with the ergodic divertor, particle injection, impedance concept for ICRF antennas, low hybrid current drive, RF systems, the 118 GHz ECRH experiment, the inner first wall, improved vacuum vessel protection, pellet injection, material activation, and the CIEL project. 3 of the additional papers concern the model coil of ITER

  12. Integral experiments performed at CEA to improve the Pu higher isotope nuclear data: PLUTO programme

    International Nuclear Information System (INIS)

    The characteristics and main results of the experimental program PLUTO, developed at CEA to improve the Pu higher isotope nuclear data, are presented. The integral experiments performed aim at providing the information necessary to adjust the multigroup cross sections used for the design calculations. The comparison of the calculation to experiment deviations on the integral parameters before and after the cross-section adjustment show the improvement brought by the PLUTO program. 6 figures, 10 tables

  13. Estructura factorial y fiabilidad de un cuestionario de estrategias de aprendizaje en universitarios: CEA-U

    OpenAIRE

    Martín Cabrera, Eduardo; García García, Luis Alberto; Torbay Betancor, Ángela; Rodríguez Blanco, Teresa

    2007-01-01

    Se analiza la estructura factorial y la consistencia interna del Cuestionario de Estrategias de Aprendizaje en Universitarios (CEA-U) a partir de las respuestas de 1105 estudiantes de segundo ciclo de carrera universitaria,. El cuestionario está formado por tres escalas: estrategias motivacionales, cognitivas y metacognitivas, en las que el análisis factorial exploratorio arroja once factores empíricos. En un análisis factorial de segundo orden, los once factores se agrupan en tres macrofacto...

  14. Differentialdiagnose beim Lungenkarzinom durch CEA, CYFRA 21-1, NSE und ProGRP

    OpenAIRE

    Gruber, Christine

    2008-01-01

    Es handelt sich um eine bizentrische, retrospektive Studie am Institut für Klinische Chemie am Klinikum Großhadern, München und an der Asklepioslungenfachklinik Gauting. Anhand von insgesamt 2749 Patienten wurde univariat und multivariat analysiert, inwieweit die Tumormarker CEA, CYFRA 21-1, NSE und ProGRP und zusätzlich die Routinelaborparameter eine Hilfestellung sowohl in der Diagnostik und Differentialdiagnostik eines Lungenrundherdes (benigne Lungenerkrankung, primäres Lungenkarzinom, Lu...

  15. EURATOM-CEA association contributions to the 21st symposium on fusion technology

    Energy Technology Data Exchange (ETDEWEB)

    Garin, P.; Grosman, A.; Beaumont, B. [and others

    2000-11-01

    The 27 contributions of EURATOM-Cea association have been gathered with 6 additional papers and 1 invited paper in this document. Most papers concern Tore-Supra and deal with the ergodic divertor, particle injection, impedance concept for ICRF antennas, low hybrid current drive, RF systems, the 118 GHz ECRH experiment, the inner first wall, improved vacuum vessel protection, pellet injection, material activation, and the CIEL project. 3 of the additional papers concern the model coil of ITER.

  16. Insights into the economic viability of a new CEA system producing hydroponic lettuce

    OpenAIRE

    Ilaslan, Gunes; White, Gerald B.; Langhans, Robert W.

    2002-01-01

    A new CEA (Controlled Environment Agriculture) system growing Boston lettuce, Lactuca Sativa cv. ‘Vivaldi’, achieves the same quality and quantity of production every day of the year. The year-round constant quantity and quality production indicate potential for success. However, our research showed that out of nine selected U.S. locations with different climatic and economic conditions, the Northeast had 25.7 percent higher production costs for the same yield. The analysis indicated that giv...

  17. Aeroacoustics research in Europe: The CEAS-ASC report on 2013 highlights

    Science.gov (United States)

    Bennett, G. J.; Kennedy, J.; Meskell, C.; Carley, M.; Jordan, P.; Rice, H.

    2015-03-01

    The Council of European Aerospace Societies (CEAS) Aeroacoustics Specialists Committee (ASC) supports and promotes the interests of the scientific and industrial aeroacoustics community on an European scale and European aeronautics activities internationally. In this context, "aeroacoustics" encompasses all aerospace acoustics and related areas. Each year the committee highlights some of the research and development projects in Europe. This paper is a report on highlights of aeroacoustics research in Europe in 2013, compiled from information provided to the ASC of the CEAS. During 2013, a number of research programmes involving aeroacoustics were funded by the European Commission. Some of the highlights from these programmes are summarised in this paper, as well as highlights from other programmes funded by national programmes or by industry. Furthermore, a concise summary of the CEAS-ASC workshop "Atmospheric and Ground Effects on Aircraft Noise" held in Seville, Spain in September 2013 is included in this report. Enquiries concerning all contributions should be addressed to the authors who are given at the end of each subsection. This issue of the "highlights" paper is dedicated to the memory of Prof. John A. Fitzpatrick, Professor of Mechanical Engineering, Trinity College Dublin, and a valued member of the Aeroacoustics Specialists Committee. John passed away in September 2012 and is fondly missed across the globe by the friends he made in the Aeroacoustics Community. This paper is edited by PhD graduates and colleagues of John's who conduct research in aeroacoustics, inspired by his thirst for knowledge.

  18. Technetium-99m labelling of the IOR CEA 1 monoclonal antibody: evaluation of different methods

    International Nuclear Information System (INIS)

    Aim: The aim of this study was to investigate the in vivo and in vitro properties of 99mTc labelled monoclonal antibody, IOR CEA 1 when radiolabelled by different methods. Methods: To achieve that purpose IOR CEA was directly radiolabelled via 2-mercaptoethanol (2-Me) and stannous ion (SnCl2) reduction and indirectly via the 2-iminothiolane (2-Im) conjugation. The resulting 99mTc-MoAbs were analysed for number of free sulfhydryl groups, chemical and radiochemical purity (checked by HPLC and SDS PAGE), immunoreactivity and biological distribution in mice. Results: Experimental results indicated a similar radiochemical purity and immunoreactivity for direct labelling methods and a decrease of both for 2-Im method. 2-Me antibody reduction led to a high antibody fragmentation as indicated by non-denaturing SDS PAGE analysis. Nevertheless SnCl2 and 2-Im labels revealed lower in vivo stability. Conclusion: 99mTc-(2-Me) IOR CEA presented favourable in vitro and in vivo properties. Therefore this label was compared to 99mTc-monoclonal antibody BW 431/26. Similar characteristics were found. Clinical studies also revealed identical biodistribution profile. (orig.)

  19. Radioimmunoimaging of ovarian cancer with 131I labelled CEA monoclonal antibody

    International Nuclear Information System (INIS)

    Objective: To investigate radioimmunoimaging with 131I labelled CEA McAb and its value in diagnosis and treatment of ovarian cancer. Methods: CEA McAb was labelled with 131I by standard chloramine T procedure. The radiolabeled McAb was given intravenously to the patients. The scintigraphy was performed at different time. Results: In 105 patients with histopathology proved ovarian cancers, for 96 patients the lesions were well located with RII (true positives), for the remaining 9 patients, the results were false negative. 22/23 cases with RII diagnosed benign ovarian tumors were proved to be true negative by surgical pathology in RII. Of 96 ovarian cancers 87 were stratified into identical stages by surgical pathology all RII. 141/151 metastatic tumors were found in RII and the positive rate was as high as 93.4%. The smallest tumor defected by RII was of 1 cm in diameter. Conclusions: RII is as good as ultrasonography and CT in distinguishing benignancy and malignancy. The location, size and number of the lesions can also be determined with RII. RII of ovarian cancer with 131I-CEA McAb is valuable and helpful in early detection of ovarian cancer. It is also helpful for clinical staging, treatment programing and prognosticating

  20. Annual report of the Association EURATOM-Cea 2005 (full report)

    International Nuclear Information System (INIS)

    This annual report summarizes activities performed by the EURATOM-Cea association in 2005. The activities carried out in the field 'physics integration' are mainly linked to the ion cyclotron range of frequency antenna development and to the development of diagnostic components. The vacuum vessel studies have mainly focused at welding techniques and at qualification of inspection methods along the vacuum vessel inter-sector weld. On the plasma facing component side investigations have been performed on material knowledge (CuCrZr creep-fatigue studies, neutron effects on material properties of CFC, development and optimisation of Be/CuCrZr joining techniques and studies dedicated to the divertor. In the field 'magnets', EURATOM-Cea association has devoted a major part of its effort to the studies of advanced Nb3Sn strands for the toroidal field coil, and the first full size conductor sample was manufactured. Within the frame of Test Blanket Module (TBM), activities mainly concerned the improvement and completion of the TBM engineering design. Within the frame of the Helium Cooled Pebble Bed concept programmes, studies about the development of Li2TiO3 pebbles are on going. The main objective of 2005 which was to improve the shape of Li2TiO3 pebbles has been successfully achieved. EURATOM-Cea maintained significant involvement in the development of structural materials for a fusion reactor. A strong effort has been made on a program of laser detritiation associated with remote handling. (A.C.)