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Sample records for anti-tlr2 intrabody mediating

  1. Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation

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    Lindenmaier Werner

    2010-04-01

    Full Text Available Abstract Background Toll-like receptor (TLR 2 is a component of the innate immune system and senses specific pathogen associated molecular patterns (PAMPs of both microbial and viral origin. Cell activation via TLR2 and other pattern recognition receptors (PRRs contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response. Intracellular antibodies expressed and retained inside the endoplasmatic reticulum (ER-intrabodies are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. Here we describe generation and application of a functional anti-TLR2 ER intrabody (αT2ib which was generated from an antagonistic monoclonal antibody (mAb towards human and murine TLR2 (T2.5 to inhibit the function of TLR2. αT2ib is a scFv fragment comprising the variable domain of the heavy chain and the variable domain of the light chain of mAb T2.5 linked together by a synthetic (Gly4Ser3 amino acid sequence. Results Coexpression of αT2ib and mouse TLR2 in HEK293 cells led to efficient retention and accumulation of TLR2 inside the ER compartment. Co-immunoprecipitation of human TLR2 with αT2ib indicated interaction of αT2ib with its cognate antigen within cells. αT2ib inhibited NF-κB driven reporter gene activation via TLR2 but not through TLR3, TLR4, or TLR9 if coexpressed in HEK293 cells. Co-transfection of human TLR2 with increasing amounts of the expression plasmid encoding αT2ib into HEK293 cells demonstrated high efficiency of the TLR2-αT2ib interaction. The αT2ib open reading frame was integrated into an adenoviral cosmid vector for production of recombinant adenovirus (AdV-αT2ib. Transduction with AdVαT2ib specifically inhibited TLR2 surface expression of murine RAW264.7 and primary macrophages derived from bone marrow (BMM. Furthermore, TLR2 activation dependent TNFα mRNA accumulation, as well

  2. 抗TLR2抗体抑制小鼠金黄色葡萄球菌肺炎炎症反应%Anti-TLR2 antibody attenuates inflammatory response of Staphylococcus aureus pneumonia in mice

    Institute of Scientific and Technical Information of China (English)

    温顺航; 林立; 李昌崇; 苏小燕; 张慧玲; 吴沪军

    2014-01-01

    in BALF and serum were assayed by ELISA ,changes in pulmonary histopathology were observed with HE staining and TLR 2 expression was detected by immunohistochemical.Results:3 days after intranasal inoculation ,the concentrations of KC and IL-10 in BALF and serum was increased in SA pneumonia mice , pulmonary histopathology changes significantly in HE staining.Compared with SA pneumonia mice,the CFU of bacteria were higher,leukocytes count and neutrophil percentage ,the concentrations of KC in BALF and serum,as well as HE pathological scores were reduced significantly in anti-TLR2 antibody group mice ,while no significant difference in IL-10.8 days after intranasal inoculation , HE pathological scores of anti-TLR2 antibody group mice were significantly lower than SA pneumonia group mice ,the CFU of bacteria in BALF were not statistically different between those two groups.Conclusion:Anti-TLR2 antibody attenuates the production of inflammatory mediators and inflammatory cell infiltration in SA pneumonia mice .

  3. Bifunctional anti-huntingtin proteasome-directed intrabodies mediate efficient degradation of mutant huntingtin exon 1 protein fragments.

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    David C Butler

    Full Text Available Huntington's disease (HD is a fatal autosomal dominant neurodegenerative disorder caused by a trinucleotide (CAG(n repeat expansion in the coding sequence of the huntingtin gene, and an expanded polyglutamine (>37Q tract in the protein. This results in misfolding and accumulation of huntingtin protein (htt, formation of neuronal intranuclear and cytoplasmic inclusions, and neuronal dysfunction/degeneration. Single-chain Fv antibodies (scFvs, expressed as intrabodies that bind htt and prevent aggregation, show promise as immunotherapeutics for HD. Intrastriatal delivery of anti-N-terminal htt scFv-C4 using an adeno-associated virus vector (AAV2/1 significantly reduces the size and number of aggregates in HDR6/1 transgenic mice; however, this protective effect diminishes with age and time after injection. We therefore explored enhancing intrabody efficacy via fusions to heterologous functional domains. Proteins containing a PEST motif are often targeted for proteasomal degradation and generally have a short half life. In ST14A cells, fusion of the C-terminal PEST region of mouse ornithine decarboxylase (mODC to scFv-C4 reduces htt exon 1 protein fragments with 72 glutamine repeats (httex1-72Q by ~80-90% when compared to scFv-C4 alone. Proteasomal targeting was verified by either scrambling the mODC-PEST motif, or via proteasomal inhibition with epoxomicin. For these constructs, the proteasomal degradation of the scFv intrabody proteins themselves was reduced<25% by the addition of the mODC-PEST motif, with or without antigens. The remaining intrabody levels were amply sufficient to target N-terminal httex1-72Q protein fragment turnover. Critically, scFv-C4-PEST prevents aggregation and toxicity of httex1-72Q fragments at significantly lower doses than scFv-C4. Fusion of the mODC-PEST motif to intrabodies is a valuable general approach to specifically target toxic antigens to the proteasome for degradation.

  4. Regulation of β2-adrenergic receptor function by conformationally selective single-domain intrabodies

    DEFF Research Database (Denmark)

    Staus, Dean P; Wingler, Laura M; Strachan, Ryan T;

    2014-01-01

    to selectively bind agonist- or antagonist-occupied receptors. When expressed as intrabodies, they inhibited G protein activation (cyclic AMP accumulation), G protein-coupled receptor kinase (GRK)-mediated receptor phosphorylation, β-arrestin recruitment, and receptor internalization to varying extents...

  5. The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells

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    Rinaldi, Anne-Sophie; Freund, Guillaume; Desplancq, Dominique; Sibler, Annie-Paule; Baltzinger, Mireille [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France); Rochel, Natacha [Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/INSERM/Université de Strasbourg, rue Laurent Fries, 67404 Illkirch (France); Mély, Yves; Didier, Pascal [Faculté de Pharmacie, UMR 7213, CNRS/Université de Strasbourg, route du Rhin, 67401 Illkirch (France); Weiss, Etienne, E-mail: eweiss@unistra.fr [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France)

    2013-04-01

    Expression of antibody fragments in mammalian cells (intrabodies) is used to probe the target protein or interfere with its biological function. We previously described the in vitro characterisation of a single-chain Fv (scFv) antibody fragment (F5) isolated from an intrabody library that binds to the oncoprotein gankyrin (GK) in solution. Here, we have isolated several other scFvs that interact with GK in the presence of F5 and tested whether they allow, when fused to fluorescent proteins, to detect by FRET endogenous GK in living cells. The binding of pairs of scFvs to GK was analysed by gel filtration and the ability of each scFv to mediate nuclear import/export of GK was determined. Binding between scFv-EGFP and RFP-labelled GK in living cells was detected by fluorescence lifetime imaging microscopy (FLIM). After co-transfection of two scFvs fused to EGFP and RFP, respectively, which form a tri-molecular complex with GK in vitro, FRET signal was measured. This system allowed us to observe that GK is monomeric and distributed throughout the cytoplasm and nucleus of several cancer cell lines. Our results show that pairs of fluorescently labelled intrabodies can be monitored by FLIM–FRET microscopy and that this technique allows the detection of lowly expressed endogenous proteins in single living cells. Highlights: ► Endogenous GK in living cells was targeted with pairs of fluorescently-tagged scFvs. ► Tri-molecular complexes containing two scFvs and one molecule GK were formed. ► GK was detected using fluorescence lifetime-based FRET imaging. ► GK is monomeric and homogeneously distributed in several cancer cell lines. ► This technique may have many applications in live-cell imaging of endogenous proteins.

  6. A novel intrabody communication transceiver for biomedical applications

    CERN Document Server

    Seyedi, Mir Hojjat

    2017-01-01

    This monograph explores Intrabody communication (IBC) as a novel non-RF wireless data communication technique using the human body itself as the communication channel or transmission medium. In particular, the book investigates Intrabody Communication considering limb joint effects within the transmission frequency range 0.3-200 MHz. Based on in-vivo experiments which determine the effects of size, situations, and locations of joints on the IBC, the book proposes a new IBC circuit model explaining elbow joint effects. This model not only takes the limb joint effects of the body into account but also considers the influence of measurement equipment in higher frequency band thus predicting signal attenuation behavior over wider frequency ranges. Finally, this work proposes transmitter and receiver architectures for intrabody communication. A carrier-free scheme based on impulse radio for the IBC is implemented on a FPGA.

  7. Electrooptic sensor module fabrication for near-field intrabody communication

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    Furuya, Akinori; Sasaki, Ai-ichiro; Morimura, Hiroki; Kagami, Osamu; Shinagawa, Mitsuru

    2014-09-01

    In this paper, we describe how to obtain a low cost electrooptic (EO) sensor module for the mass production of near-field intrabody communication devices. In our previous study, we used a bulk cleavage technique to fabricate EO modulators without the need for any optical polishing or washing processes. In this study, we fabricated EO modulators as a miniaturized chip sensor without a base portion, and clarified the feasibility of assembling optical components by only a passive alignment technique with a compact housing.

  8. TLR2单克隆抗体对大鼠角膜移植术后植片存活的保护作用%Protective role of anti-TLR2 monoclonal antibody to corneal graft survival after allograft corneal transplantation in rats

    Institute of Scientific and Technical Information of China (English)

    白浪; 郑艳华; 梁伟怡

    2015-01-01

    Background The effects of Toll-like receptor 2 (TLR2) in grafting-related immune diseases have attracted more and more attention.Blocking TLR2 signal pathway can extend the survival time of heart and kidney grafts.However, the effects of anti-TLR2 monoclonal antibody on corneal graft have not been confirmed.Objective This study was to investigate the influence of anti-TLR2 monoclonal antibody on corneal graft survival in the rats received penetrating keratoplasty (PKP).Methods Allograft corneal transplantation was performed on the right eyes of 24 SPF female Wistar rats to establish PKP models,with 12 SD rats as donors.The model eyes were randomized into the TLR2 monoclonal antibody group and the model group.Anti-TLR2 monoclonal antibody of 15 μg/30 μl was subconjunctivally injected on day 0,2,4,6 and 8 following the modeling in the TLR2 monoclonal antibody group,and equal amount of normal saline was injected in the same way in the model group.The edema,transparency and neovascularization were observed under the slit lamp microscope after surgery, and rejection index (RI) was scored based on the criteria of Holland.Corneal tissue sections of the rats were prepared for the histopathological examination on day 9 and 15 after operation.The research protocol was approved by the Southern Medical University Ethics Committee.Results Mild corneal edema was found in the two groups 1-4 days after operation.A lot of new blood vessels, edema and opacification of corneas were seen in the model group 9-14 days after operation,but in the TLR2 monoclonal antibody group,corneal opacification was found 15 days after operation.The RI scores were significantly higher in the model group than those in the TLR2 monoclonal antibody group 5,9,15 days after operation (t=4.183,4.954,13.506;all at P<0.05).The survival time in the TLR2 monoclonal antibody group was 15.5 days,with the 95% confidence interval (CI) 14.9-16.1;while that in the model group was 9.5 days,with the 95% CI 8

  9. Cascaded Network Body Channel Model for Intrabody Communication.

    Science.gov (United States)

    Wang, Hao; Tang, Xian; Choy, Chiu Sing; Sobelman, Gerald E

    2016-07-01

    Intrabody communication has been of great research interest in recent years. This paper proposes a novel, compact but accurate body transmission channel model based on RC distribution networks and transmission line theory. The comparison between simulation and measurement results indicates that the proposed approach accurately models the body channel characteristics. In addition, the impedance-matching networks at the transmitter output and the receiver input further maximize the power transferred to the receiver, relax the receiver complexity, and increase the transmission performance. Based on the simulation results, the power gain can be increased by up to 16 dB after matching. A binary phase-shift keying modulation scheme is also used to evaluate the bit-error-rate improvement.

  10. Suppression of p75 neurotrophin receptor surface expression with intrabodies influences Bcl-xL mRNA expression and neurite outgrowth in PC12 cells.

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    Congcong Zhang

    Full Text Available BACKGROUND: Although p75 neurotrophin receptor (p75NTR is the first neurotrophin receptor isolated, its diverse physiological functions and signaling have remained elusive for many years. Loss-of-function phenotypic analyses for p75NTR were mainly focused at the genetic level; however these approaches were impacted by off-target effect, insufficient stability, unspecific stress response or alternative active splicing products. In this study, p75NTR surface expression was suppressed for the first time at the protein level by endoplasmic reticulum (ER retained intrabodies. RESULTS: Three monoclonal recombinant antibody fragments (scFv with affinities in the low nanomolar range to murine p75NTR were isolated by antibody phage display. To suppress p75NTR cell surface expression, the encoding genes of these scFvs extended by the ER retention peptide KDEL were transiently transfected into the neuron-like rat pheochromocytoma cell line PC12 and the mouse neuroblastoma x mouse spinal cord hybrid cell line NSC19. The ER retained intrabody construct, SH325-G7-KDEL, mediated a downregulation of p75NTR cell surface expression as shown by flow cytometry. This effect was maintained over a period of at least eight days without activating an unfolded protein response (UPR. Moreover, the ER retention of p75NTR resulted in downregulation of mRNA levels of the anti-apoptotic protein Bcl-xL as well as in strong inhibition of NGF-induced neurite outgrowth in PC12 cells. CONCLUSION: The ER retained intrabody SH325-G7-KDEL not only induces phenotypic knockdown of this p75NTR but also p75NTR-associated cellular responses in PC12 cells.

  11. Health Monitoring System Based on Intra-Body Communication

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    Razak, A. H. A.; Ibrahim, I. W.; Ayub, A. H.; Amri, M. F.; Hamzi, M. H.; Halim, A. K.; Ahmad, A.; Junid, S. A. M. Al

    2015-11-01

    This paper presents a model of a Body Area Network (BAN) health monitoring system based on Intra-Body Communication. Intra-body Communication (IBC) is a communication technique that uses the human body as a medium for electrical signal communication. One of the visions in the health care industry is to provide autonomous and continuous self and the remote health monitoring system. This can be achieved via BAN, LAN and WAN integration. The BAN technology itself consists of short range data communication modules, sensors, controller and actuators. The information can be transmitted to the LAN and WAN via the RF technology such as Bluetooth, ZigBee and ANT. Although the implementations of RF communication have been successful, there are still limitations in term of power consumption, battery lifetime, interferences and signal attenuations. One of the solutions for Medical Body Area Network (MBANs) to overcome these issues is by using an IBC technique because it can operate at lower frequencies and power consumption compared to the existing techniques. The first objective is to design the IBC's transmitter and receiver modules using the off the shelf components. The specifications of the modules such as frequency, data rate, modulation and demodulation coding system were defined. The individual module were designed and tested separately. The modules was integrated as an IBC system and tested for functionality then was implemented on PCB. Next objective is to model and implement the digital parts of the transmitter and receiver modules on the Altera's FPGA board. The digital blocks were interfaced with the FPGA's on board modules and the discrete components. The signals that have been received from the transmitter were converted into a proper waveform and it can be viewed via external devices such as oscilloscope and Labview. The signals such as heartbeats or pulses can also be displayed on LCD. In conclusion, the IBC project presents medical health monitoring model

  12. Signal measurement system for intra-body communication using optical isolation method

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    Matsumoto, Kazuki; Katsuyama, Jun; Sugiyama, Ryo; Takizawa, Yasuaki; Ishii, Seita; Shinagawa, Mitsuru; Kado, Yuichi

    2014-09-01

    In this paper, we describe an induced signal measurement on the human body for developing a high-performance transceiver of an intra-body communication system. It is important to isolate awearable transceiver from an electrical instrument for precise measurement. We have developed a probe system using an optical isolation method including a laser diode, photo-diode, and optical fiber. The probe system can be successfully applied to the precise measurement of a receiving signal power at a wearable transceiver. We verify that the experimental results agree with the simulation results based on our previous channel model of intra-body communication.

  13. Modeling for intra-body communication with bone effect.

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    Pun, S H; Gao, Y M; Mak, P U; Du, M; Vai, M I

    2009-01-01

    Intra-body communication (IBC) is a new, different "wireless" communication technique based on the human tissue. This short range "wireless" communication technology provides an alternative solution to wearable sensors, home health system, telemedicine and implanted devices. The development of the IBC enables the possibilities of providing less complexity and convenient communication methodologies for these devices. By regarding human tissue as communication channel, IBC making use of the conductivities properties of human tissue to send electrical signal from transmitter to receiver. In this paper, the authors proposed a new mathematical model for galvanic coupling type IBC based on a human limb. Starting from the electromagnetic theory, the authors treat human tissue as volume conductor, which is in analogous with the bioelectric phenomena analysis. In order to explain the mechanism of galvanic coupling type technique of IBC, applying the quasi-static approximation, the governing equation can be reduced to Laplace Equation. Finally, the analytical model is evaluated with on-body measurement for testing its performance. The comparison result shows that the developed mathematical model can provide good approximation for galvanic coupling type IBC on human limb under low operating frequencies.

  14. A stably expressed llama single-domain intrabody targeting Rev displays broad-spectrum anti-HIV activity.

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    Boons, Eline; Li, Guangdi; Vanstreels, Els; Vercruysse, Thomas; Pannecouque, Christophe; Vandamme, Anne-Mieke; Daelemans, Dirk

    2014-12-01

    The HIV Rev protein mediates the transport of partially and unspliced HIV mRNA from the nucleus to the cytoplasm. Rev multimerizes on a secondary stem-loop structure present in the viral intron-containing mRNA species and recruits the cellular karyopherin CRM1 to export viral mRNAs from the nucleus to the cytoplasm. Previously we have identified a single-domain intrabody (Nb(190)), derived from a llama heavy-chain antibody, which efficiently inhibits Rev multimerization and suppresses the production of infectious virus. We recently mapped the epitope of this nanobody and demonstrated that Rev residues K20 and Y23 are crucial for interaction while residues V16, H53 and L60 are important to a lesser extent. Here, we generated cell lines stably expressing Nb(190) and assessed the capacity of these cell lines to suppress the replication of different HIV-1 subtypes. These cells stably expressing the single-domain antibody are protected from virus-induced cytopathogenic effect even in the context of high multiplicity of infection. In addition, the replication of different subtypes of group M and one strain of group O is significantly suppressed in these cell lines. Next, we analysed the natural variations of Rev amino acids in sequence samples from HIV-1 infected patients worldwide and assessed the effect of Nb(190) on the most prevalent polymorphisms occurring at the key epitope positions (K20 and Y23) in Rev. We found that Nb(190) was able to suppress the function of these Rev variants except for the K20N mutant, which was present in only 0.7% of HIV-1 sequence populations (n = 4632). Cells stably expressing the single-domain intrabody Nb(190) are protected against virus-induced cytopathogenic effect and display a selective survival advantage upon infection. In addition, Nb(190) suppresses the replication of a wide range of different HIV-1 subtypes. Large-scale sequence analysis reveals that the Nb(190) epitope positions in Rev are well conserved across major HIV-1

  15. Anti-WASP intrabodies inhibit inflammatory responses induced by Toll-like receptors 3, 7, and 9, in macrophages

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    Sakuma, Chisato [Animal Immune and Cell Biology Research Unit, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634 (Japan); Sato, Mitsuru, E-mail: mitsuru.sato@affrc.go.jp [Animal Immune and Cell Biology Research Unit, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634 (Japan); Oshima, Takuma [Department of Biological Science and Technology, Graduate School of Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510 (Japan); Takenouchi, Takato [Animal Immune and Cell Biology Research Unit, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634 (Japan); Chiba, Joe [Department of Biological Science and Technology, Graduate School of Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510 (Japan); Kitani, Hiroshi [Animal Immune and Cell Biology Research Unit, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634 (Japan)

    2015-02-27

    Wiskott-Aldrich syndrome protein (WASP) is an adaptor molecule in immune cells. Recently, we showed that the WASP N-terminal domain interacted with the SH3 domain of Bruton's tyrosine kinase (Btk), and that the complex formed by WASP and Btk was important for TLR2 and TLR4 signaling in macrophages. Several other studies have shown that Btk played important roles in modulating innate immune responses through TLRs in immune cells. Here, we evaluated the significance of the interaction between WASP and Btk in TLR3, TLR7, and TLR9 signaling. We established bone marrow–derived macrophage cell lines from transgenic (Tg) mice that expressed intracellular antibodies (intrabodies) that specifically targeted the WASP N-terminal domain. One intrabody comprised the single-chain variable fragment and the other comprised the light-chain variable region single domain of an anti-WASP N-terminal monoclonal antibody. Both intrabodies inhibited the specific interaction between WASP and Btk, which impaired the expression of TNF-α, IL-6, and IL-1β in response to TLR3, TLR7, or TLR9 stimulation. Furthermore, the intrabodies inhibited the phosphorylation of both nuclear factor (NF)-κB and WASP in response to TLR3, TLR7, or TLR9 stimulation, in the Tg bone marrow-derived macrophages. These results suggested that WASP plays important roles in TLR3, TLR7, and TLR9 signaling by associating with Btk in macrophages. - Highlights: • The interaction between WASP and Btk is critical for TLR3, TLR7, and TLR9 signaling. • Anti-WASP intrabodies inhibited several TLR pathways that led to cytokine expression. • Phosphorylation of NF-κB via TLR signaling was inhibited by anti-WASP intrabodies. • WASP phosphorylation via several TLR ligands was inhibited by anti-WASP intrabodies.

  16. An intra-body molecular communication networks framework for continuous health monitoring and diagnosis.

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    Chahibi, Youssef; Balasingham, Ilangko

    2015-01-01

    Intra-body communication networks are designed to interconnect nano- or micro-sized sensors located inside the body for health monitoring and drug delivery. The most promising solutions are made of implanted nanosensors to timely monitor the body for the presence of specific diseases and pronounce a diagnosis without the intervention of a physician. In this manner, several deadly health conditions such as heart attacks are avoided through the early in vivo detection of their biomarkers. In reality, nanosensors are challenged by the individual specificities, molecular noise, limited durability, and low energy resources. In this paper, a framework is proposed for estimating and detecting diseases and localizing the nanosensors. This framework is based on molecular communication, a novel communication paradigm where information is conveyed through molecules. Through the case study of the shedding of endothelial cells as an early biomarker for heart attack, the intra-body molecular communication networks framework is shown to resolve major issues with in vivo nanosensors and lay the foundations of low-complexity biomedical signal processing algorithms for continuous disease monitoring and diagnosis.

  17. Simple electrical model and initial experiments for intra-body communications.

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    Gao, Y M; Pun, S H; Du, M; Mak, P U; Vai, M I

    2009-01-01

    Intra-Body Communication(IBC) is a short range "wireless" communication technique appeared in recent years. This technique relies on the conductive property of human tissue to transmit the electric signal among human body. This is beneficial for devices networking and sensors among human body, and especially suitable for wearable sensors, telemedicine system and home health care system as in general the data rates of physiologic parameters are low. In this article, galvanic coupling type IBC application on human limb was investigated in both its mathematical model and related experiments. The experimental results showed that the proposed mathematical model was capable in describing the galvanic coupling type IBC under low frequency. Additionally, the calculated result and experimental result also indicated that the electric signal induced by the transmitters of IBC can penetrate deep into human muscle and thus, provide an evident that IBC is capable of acting as networking technique for implantable devices.

  18. Investigation of galvanic-coupled intrabody communication using the human body circuit model.

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    Kibret, Behailu; Seyedi, MirHojjat; Lai, Daniel T H; Faulkner, Micheal

    2014-07-01

    Intrabody Communication (IBC) is a technique that uses the human body as a transmission medium for electrical signals to connect wearable electronic sensors and devices. Understanding the human body as the transmission medium in IBC paves way for practical implementation of IBC in body sensor networks. In this study, we propose a model for galvanic coupling-type IBC based on a simplified equivalent circuit representation of the human upper arm. We propose a new way to calculate the electrode-skin contact impedance. Based on the model and human experimental results, we discuss important characteristics of galvanic coupling-type IBC, namely, the effect of tissues, anthropometry of subjects, and electrode configuration on signal propagation. We found that the dielectric properties of the muscle primarily characterize the received signal when receiver electrodes are located close to transmitter electrodes. When receiver and transmitter electrodes are far apart, the skin dielectric property affects the received signal.

  19. Application of Near-field intra-body communication and spread spectrum technique to vital-sign monitor.

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    Kobayashi, Takumi; Shimatani, Yuichi; Kyoso, Masaki

    2012-01-01

    As a novel vital sign monitor, we have developed wireless ECG monitoring system with Near-field intra-body communication technique. However, communication reliability is not so high because transmission channel is noisy and unstable. In order to improve the problem, we utilize spread spectrum (SS), which is known as robust communication technique even through poor transmission channel. First of all, we evaluated characteristics of human body to SS signal. The results show that SS can be used even through human body. Based on this result, we developed and tested near-field intra-body communication device enhanced by SS. The test result shows that SS can solve the problem mentioned above.

  20. A finite-element simulation of galvanic coupling intra-body communication based on the whole human body.

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    Song, Yong; Zhang, Kai; Hao, Qun; Hu, Lanxin; Wang, Jingwen; Shang, Fuzhou

    2012-10-09

    Simulation based on the finite-element (FE) method plays an important role in the investigation of intra-body communication (IBC). In this paper, a finite-element model of the whole body model used for the IBC simulation is proposed and verified, while the FE simulation of the galvanic coupling IBC with different signal transmission paths has been achieved. Firstly, a novel finite-element method for modeling the whole human body is proposed, and a FE model of the whole human body used for IBC simulation was developed. Secondly, the simulations of the galvanic coupling IBC with the different signal transmission paths were implemented. Finally, the feasibility of the proposed method was verified by using in vivo measurements within the frequency range of 10 kHz-5 MHz, whereby some important conclusions were deduced. Our results indicate that the proposed method will offer significant advantages in the investigation of the galvanic coupling intra-body communication.

  1. Effect of Human Movement on Galvanic Intra-Body Communication during Single Gait Cycle

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    Ibrahim, I. W.; Razak, A. H. A.; Ahmad, A.; Salleh, M. K. M.

    2015-11-01

    Intra-body communication (IBC) is a communication system that uses human body as a signal transmission medium. From previous research, two coupling methods of IBC were concluded which are capacitive coupling and galvanic coupling. This paper investigates the effect of human movement on IBC using the galvanic coupling method. Because the human movement is control by the limb joint, the knee flexion angle during gait cycle was used to examine the influence of human movement on galvanic coupling IBC. The gait cycle is a cycle of people walking that start from one foot touch the ground till that foot touch the ground again. Frequency range from 300 kHz to 200MHz was swept in order to investigate the signal transmission loss and the result was focused on operating frequency 70MHz to 90MHz. Results show that the transmission loss varies when the knee flexion angle increased. The highest loss of signal at frequency range between 70MHz to 90 MHz was 69dB when the knee flexion angle is 50° and the minimum loss was 51dB during the flexion angle is 5°.

  2. Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells

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    Mai Sabine

    2008-01-01

    Full Text Available Abstract Background Expression of intracellular antibodies (intrabodies has become a broadly applicable technology for generation of phenotypic knockouts in vivo. The method uses surface depletion of cellular membrane proteins to examine their biological function. In this study, we used this strategy to block the transport of cell surface molecule CD147 to the cell membrane. Phage display technology was introduced to generate the functional antibody fragment to CD147, and we subsequently constructed a CD147-specific scFv that was expressed intracellularly and retained in the endoplasmic reticulum by adenoviral gene transfer. Results The recombinant antibody fragments, Fab and scFv, of the murine monoclonal antibody (clone M6-1B9 reacted specifically to CD147 by indirect enzyme-linked immunosorbent assays (ELISA using a recombinant CD147-BCCP as a target. This indicated that the Fab- and scFv-M6-1B9 displaying on phage surfaces were correctly folded and functionally active. We subsequently constructed a CD147-specific scFv, scFv-M6-1B9-intrabody, in 293A cells. The expression of CD147 on 293A cell surface was monitored at 36 h after transduction by flow cytometry and demonstrated remarkable reduction. Colocalization of scFv-M6-1B9 intrabody with CD147 in the ER network was depicted using a 3D deconvolution microscopy system. Conclusion The results suggest that our approach can generate antibody fragments suitable for decreasing the expression of CD147 on 293A cells. This study represents a step toward understanding the role of the cell surface protein, CD147.

  3. Characterization of Impulse Radio Intrabody Communication System for Wireless Body Area Networks.

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    Cai, Zibo; Seyedi, MirHojjat; Zhang, Weiwei; Rivet, Francois; Lai, Daniel T H

    2017-01-01

    Intrabody communication (IBC) is a promising data communication technique for body area networks. This short-distance communication approach uses human body tissue as the medium of signal propagation. IBC is defined as one of the physical layers for the new IEEE 802.15.6 or wireless body area network (WBAN) standard, which can provide a suitable data rate for real-time physiological data communication while consuming lower power compared to that of radio-frequency protocols such as Bluetooth. In this paper, impulse radio (IR) IBC (IR-IBC) is examined using a field-programmable gate array (FPGA) implementation of an IBC system. A carrier-free pulse position modulation (PPM) scheme is implemented using an IBC transmitter in an FPGA board. PPM is a modulation technique that uses time-based pulse characteristics to encode data based on IR concepts. The transmission performance of the scheme was evaluated through signal propagation measurements of the human arm using 4- and 8-PPM transmitters, respectively. 4 or 8 is the number of symbols during modulations. It was found that the received signal-to-noise ratio (SNR) decreases approximately 8.0 dB for a range of arm distances (5-50 cm) between the transmitter and receiver electrodes with constant noise power and various signal amplitudes. The SNR for the 4-PPM scheme is approximately 2 dB higher than that for the 8-PPM one. In addition, the bit error rate (BER) is theoretically analyzed for the human body channel with additive white Gaussian noise. The 4- and 8-PPM IBC systems have average BER values of 10(-5) and 10(-10), respectively. The results indicate the superiority of the 8-PPM scheme compared to the 4-PPM one when implementing the IBC system. The performance evaluation of the proposed IBC system will improve further IBC transceiver design.

  4. Characterization of an In Vivo Z-DNA Detection Probe Based on a Cell Nucleus Accumulating Intrabody.

    Science.gov (United States)

    Gulis, Galina; Silva, Izabel Cristina Rodrigues; Sousa, Herdson Renney; Sousa, Isabel Garcia; Bezerra, Maryani Andressa Gomes; Quilici, Luana Salgado; Maranhao, Andrea Queiroz; Brigido, Marcelo Macedo

    2016-09-01

    Left-handed Z-DNA is a physiologically unstable DNA conformation, and its existence in vivo can be attributed to localized torsional distress. Despite evidence for the existence of Z-DNA in vivo, its precise role in the control of gene expression is not fully understood. Here, an in vivo probe based on an anti-Z-DNA intrabody is proposed for native Z-DNA detection. The probe was used for chromatin immunoprecipitation of potential Z-DNA-forming sequences in the human genome. One of the isolated putative Z-DNA-forming sequences was cloned upstream of a reporter gene expression cassette under control of the CMV promoter. The reporter gene encoded an antibody fragment fused to GFP. Transient co-transfection of this vector along with the Z-probe coding vector improved reporter gene expression. This improvement was demonstrated by measuring reporter gene mRNA and protein levels and the amount of fluorescence in co-transfected CHO-K1 cells. These results suggest that the presence of the anti-Z-DNA intrabody can interfere with a Z-DNA-containing reporter gene expression. Therefore, this in vivo probe for the detection of Z-DNA could be used for global correlation of Z-DNA-forming sequences and gene expression regulation.

  5. Targeting the hepatitis B virus precore antigen with a novel IgNAR single variable domain intrabody.

    Science.gov (United States)

    Walsh, Renae; Nuttall, Stewart; Revill, Peter; Colledge, Danni; Cabuang, Liza; Soppe, Sally; Dolezal, Olan; Griffiths, Kate; Bartholomeusz, Angeline; Locarnini, Stephen

    2011-03-01

    The Hepatitis B virus precore protein is processed in the endoplasmic reticulum (ER) into secreted hepatitis B e antigen (HBeAg), which acts as an immune tolerogen to establish chronic infection. Downregulation of secreted HBeAg should improve clinical outcome, as patients who effectively respond to current treatments (IFN-α) have significantly lower serum HBeAg levels. Here, we describe a novel reagent, a single variable domain (V(NAR)) of the shark immunoglobulin new antigen receptor (IgNAR) antibodies. V(NAR)s possess advantages in stability, size (~14 kDa) and cryptic epitope recognition compared to conventional antibodies. The V(NAR) domain displayed biologically useful affinity for recombinant and native HBeAg, and recognised a unique conformational epitope. To assess therapeutic potential in targeting intracellular precore protein to reduce secreted HBeAg, the V(NAR) was engineered for ER-targeted in vitro delivery to function as an intracellular antibody (intrabody). In vitro data from HBV/precore hepatocyte cell lines demonstrated effective intrabody regulation of precore/HBeAg.

  6. A single-chain variable fragment intrabody prevents intracellular polymerization of Z α1-antitrypsin while allowing its antiproteinase activity.

    Science.gov (United States)

    Ordóñez, Adriana; Pérez, Juan; Tan, Lu; Dickens, Jennifer A; Motamedi-Shad, Neda; Irving, James A; Haq, Imran; Ekeowa, Ugo; Marciniak, Stefan J; Miranda, Elena; Lomas, David A

    2015-06-01

    Mutant Z α1-antitrypsin (E342K) accumulates as polymers within the endoplasmic reticulum (ER) of hepatocytes predisposing to liver disease, whereas low levels of circulating Z α1-antitrypsin lead to emphysema by loss of inhibition of neutrophil elastase. The ideal therapy should prevent polymer formation while preserving inhibitory activity. Here we used mAb technology to identify interactors with Z α1-antitrypsin that comply with both requirements. We report the generation of an mAb (4B12) that blocked α1-antitrypsin polymerization in vitro at a 1:1 molar ratio, causing a small increase of the stoichiometry of inhibition for neutrophil elastase. A single-chain variable fragment (scFv) intrabody was generated based on the sequence of mAb4B12. The expression of scFv4B12 within the ER (scFv4B12KDEL) and along the secretory pathway (scFv4B12) reduced the intracellular polymerization of Z α1-antitrypsin by 60%. The scFv4B12 intrabody also increased the secretion of Z α1-antitrypsin that retained inhibitory activity against neutrophil elastase. MAb4B12 recognized a discontinuous epitope probably located in the region of helices A/C/G/H/I and seems to act by altering protein dynamics rather than binding preferentially to the native state. This novel approach could reveal new target sites for small-molecule intervention that may block the transition to aberrant polymers without compromising the inhibitory activity of Z α1-antitrypsin.

  7. Electro-Optical Sensor Fabricated Using a Bulk Cleavage Technique and Its Characteristics for Near-Field Intra-Body Communication

    Science.gov (United States)

    Furuya, Akinori; Sasaki, Ai-ichiro; Morimura, Hiroki; Aihara, Kimihisa; Shinagawa, Mitsuru

    2013-09-01

    This paper describes how to obtain a low cost electro-optical (EO) sensor module for the mass production of near-field intra-body communication devices. In this study, we used a bulk cleavage technique to fabricate EO modulators without the need for any optical polishing or washing processes, and clarified the feasibility of assembling optical components using only a passive alignment technique with a compact housing.

  8. Bit error rate estimation for galvanic-type intra-body communication using experimental eye-diagram and jitter characteristics.

    Science.gov (United States)

    Li, Jia Wen; Chen, Xi Mei; Pun, Sio Hang; Mak, Peng Un; Gao, Yue Ming; Vai, Mang I; Du, Min

    2013-01-01

    Bit error rate (BER), which indicates the reliability of communicate channel, is one of the most important values in all kinds of communication system, including intra-body communication (IBC). In order to know more about IBC channel, this paper presents a new method of BER estimation for galvanic-type IBC using experimental eye-diagram and jitter characteristics. To lay the foundation for our methodology, the fundamental relationships between eye-diagram, jitter and BER are first reviewed. Then experiments based on human lower arm IBC are carried out using quadrature phase shift keying (QPSK) modulation scheme and 500 KHz carries frequency. In our IBC experiments, the symbol rate is from 10 Ksps to 100 Ksps, with two transmitted power settings, 0 dBm and -5 dBm. Finally, the BER results were obtained after calculation by experimental data through the relationships among eye-diagram, jitter and BER. These results are then compared with theoretical values and they show good agreement, especially when SNR is between 6 dB to 11 dB. Additionally, these results demonstrate assuming the noise of galvanic-type IBC channel as Additive White Gaussian Noise (AWGN) in previous study is applicable.

  9. 植入式医学传感器体内通信的建模与分析%Modeling and analysis of the intra-body communication of implantable medical sensors

    Institute of Scientific and Technical Information of China (English)

    高跃明; 潘少恒; 麦炳源; 韦孟宇; 杜民

    2012-01-01

    The human channel transmission characteristics of intra-body communication of implantable medical sensors are studied. A quasi-static field model of intra-body communication of implantable medical sensors is established using finite element method. An experiment platform was designed, which is used to verify the correctness of the model. The electrical potential gains are calculated as the intra-body electrical signal transmits along axial and radial directions when the model conductivity has isotropic and anisotropic characteristics, respectively. The results show that the intra-body communication signal attenuates obviously along the axial direction in the quasi-static frequency band, and the signal transmits stably along the radial direction. And the influence of frequency variation on signal gain is small; moreover,the signal gain for the anisotropic conductivity is obviously larger than that for the isotropic situation. The signal gains for intra-body communication and radio communication are compared,and the signal gain for intra-body communication is superior.%研究植入式医学传感器体内通信的人体信道传输特性.采用有限元方法建立植入式医学传感器体内通信的准静态场模型,设计实验平台验证模型的正确性,计算出模型电导率分别为各向同性和各向异性时体内电信号沿轴向和径向传输的电位增益.结果表明,植入式医学传感器的体内通信信号在准静态场频段内沿轴向衰减明显,沿径向稳定传输;信号增益受频率变化的影响较小;当电导率为各向异性时信号增益明显大于各项同性的情况;并比较了体内通信对射频通信在信号增益上的优势.

  10. A study on reception electrodes for the vital-sign monitor using near-field intra-body communication enhanced by spread spectrum technique.

    Science.gov (United States)

    Kobayashi, Takumi; Shimatani, Yuichi; Kyoso, Masaki

    2013-01-01

    As a novel vital sign monitor, we have developed wireless ECG monitoring system with Near-field intra-body communication (NF-IBC) technique. However, it was hard to ensure communication reliability because transmission channel is noisy and unstable. In order to solve the problem, we utilize spread spectrum (SS), which is known as robust communication technique even through poor transmission channel. In previous study, we have already developed an ECG monitor using NF-IBC enhanced by SS. In this paper, we evaluated on structure of the reception electrode for reliable communication. Based on the evaluations with bit error rate, we suggested the reception electrode structure which can keep the communication reliability. As the results we considered that we can expand the reception electrode up to 2.25 m(2). Moreover, we proposed the structure of the reception electrodes that can keep the communication reliability. Finally we suggested how to use the SS NF-IBC vital-sign monitor in room that larger than 2.25 m(2), and we had shown the practicability of the systems.

  11. Study of Channel Characteristics for Galvanic-Type Intra-Body Communication Based on a Transfer Function from a Quasi-Static Field Model

    Science.gov (United States)

    Chen, Xi Mei; Mak, Peng Un; Pun, Sio Hang; Gao, Yue Ming; Lam, Chan-Tong; Vai, Mang I.; Du, Min

    2012-01-01

    Intra-Body Communication (IBC), which modulates ionic currents over the human body as the communication medium, offers a low power and reliable signal transmission method for information exchange across the body. This paper first briefly reviews the quasi-static electromagnetic (EM) field modeling for a galvanic-type IBC human limb operating below 1 MHz and obtains the corresponding transfer function with correction factor using minimum mean square error (MMSE) technique. Then, the IBC channel characteristics are studied through the comparison between theoretical calculations via this transfer function and experimental measurements in both frequency domain and time domain. High pass characteristics are obtained in the channel gain analysis versus different transmission distances. In addition, harmonic distortions are analyzed in both baseband and passband transmissions for square input waves. The experimental results are consistent with the calculation results from the transfer function with correction factor. Furthermore, we also explore both theoretical and simulation results for the bit-error-rate (BER) performance of several common modulation schemes in the IBC system with a carrier frequency of 500 kHz. It is found that the theoretical results are in good agreement with the simulation results. PMID:23443387

  12. Effects of muscle conductivity on signal transmission of intra-body communications%肌肉电导率对人体通信信号传输的影响

    Institute of Scientific and Technical Information of China (English)

    曾雪芝; 高跃明; 潘少恒; 麦炳源; 韦孟宇; 杜民

    2013-01-01

    肌肉组织具有良好的电导率且占据人体较大体积,研究肌肉电导率在人体通信中的作用,有助于掌握电流耦合信号传输特性,提高人体建模的准确性.采用有限元法建立电流耦合信号在肢体传输的准静态场模型,研究不同频率下肌肉电导率的变化引起各组织层总电流密度分布的变化,重点分析肌肉各向异性电导率对耦合电流信号传输的影响,最后通过实验测量验证有限元模型.结果表明,超过64%的电流信号流经肌肉层,并且肌肉电导率越大流经肌肉层的电流越多;在1kHz~1MHz范围内,肌肉各向异性电导率对人体信号传输有较大影响;考虑肌肉电导率各向异性的模型更接近人体实验结果.%The muscle occupies the large volume of the human body and has good electrical conductivity. The research on effect of muscle conductivity in intra-body communication can help for analyzing the transmission characteristics of current coupling signal, as well as improving the accuracy of model. The quasi-static model based on the finite element method (FEM) was established and analyzed, for current coupling signal transmission through the limb. The variation of total current density distribution resulted from the muscle conductivity at different frequency was studied. Then the effects of muscle anisotropic conductivity were emphatically analyzed. Finally, the FEM model was verified by experimental measurements. The results showed that more than 64% of the current signal transmitted through the muscle layer. And the larger muscle conductivity, the more current propagates through the muscle. In 1 kHz ~1 MHz, the anisotropic conductivity of muscle has a great effect on signal transmission of intra-body communications. What's more , the model with muscle anisotropic conductivity closer to real human body.

  13. Intercultural Mediation

    OpenAIRE

    Dragos Marian Radulescu; Denisa Mitrut

    2012-01-01

    The Intercultural Mediator facilitates exchanges between people of different socio-cultural backgrounds and acts as a bridge between immigrants and national and local associations, health organizations, services and offices in order to foster integration of every single individual. As the use mediation increases, mediators are more likely to be involved in cross-cultural mediation, but only the best mediators have the opportunity to mediate cross border business disputes or international poli...

  14. Mediatized Humanitarianism

    DEFF Research Database (Denmark)

    Vestergaard, Anne

    2014-01-01

    The article investigates the implications of mediatization for the legitimation strategies of humanitarian organizations. Based on a (full population) corpus of ~400 pages of brochure material from 1970 to 2007, the micro-textual processes involved in humanitarian organizations' efforts to legiti......The article investigates the implications of mediatization for the legitimation strategies of humanitarian organizations. Based on a (full population) corpus of ~400 pages of brochure material from 1970 to 2007, the micro-textual processes involved in humanitarian organizations' efforts...... legitimation by accountancy, legitimation by institutionalization, and legitimation by compensation. The analysis relates these changes to a problem of trust associated with mediatization through processes of mediation....

  15. Complex Mediation

    DEFF Research Database (Denmark)

    Bødker, Susanne; Andersen, Peter Bøgh

    2005-01-01

    This article has its starting point in a large number of empirical findings regarding computer-mediated work. These empirical findings have challenged our understanding of the role of mediation in such work; on the one hand as an aspect of communication and cooperation at work and on the other hand...... as an aspect of human engagement with instruments of work. On the basis of previous work in activity-theoretical and semiotic human—computer interaction, we propose a model to encompass both of these aspects. In a dialogue with our empirical findings we move on to propose a number of types of mediation...... that have helped to enrich our understanding of mediated work and the design of computer mediation for such work....

  16. Axion Mediation

    CERN Document Server

    Baryakhtar, Masha; March-Russell, John

    2013-01-01

    We explore the possibility that supersymmetry breaking is mediated to the Standard Model sector through the interactions of a generalized axion multiplet that gains a F-term expectation value. Using an effective field theory framework we enumerate the most general possible set of axion couplings and compute the Standard Model sector soft-supersymmetry-breaking terms. Unusual, non-minimal spectra, such as those of both natural and split supersymmetry are easily implemented. We discuss example models and low-energy spectra, as well as implications of the particularly minimal case of mediation via the QCD axion multiplet. We argue that if the Peccei-Quinn solution to the strong-CP problem is realized in string theory then such axion-mediation is generic, while in a field theory model it is a natural possibility in both DFSZ- and KSVZ-like regimes. Axion mediation can parametrically dominate gravity-mediation and is also cosmologically beneficial as the constraints arising from axino and gravitino overproduction ...

  17. Mediatized Parenthood

    DEFF Research Database (Denmark)

    Sonne Damkjær, Maja

    2017-01-01

    to parenthood? The dissertation explores this question on the basis of a synchronous study within an overall mediatization perspective. The first part of the dissertation focuses on a conceptualization of the relationship between digital media and parenting as well as an exploration of theoretical perspectives...... and methods that make it possible to study the interactions between the two. Concretely, the dissertation builds on a number of key studies within audience research, which have contributed knowledge about the media’s role in the family and the home. This is done by including three approaches to mediatization......) a family-oriented, b) a peer-oriented, c) an oppositional, and d) non-use. Secondary contribution: Based on qualitative audience research and mediatization theory, the dissertation contributes a conceptualization of the relationship between media and parenthood. This is carried out in a study design...

  18. Mediating Business

    DEFF Research Database (Denmark)

    "Mediating Business" is a study of the expansion of business journalism. Building on evidence from Denmark, Finland, Norway and Sweden, "Mediating Business" is a comparative and multidisciplinary study of one of the major transformations of the mass media and the realm of business - nationally...... and globally. The book explores the history of key innovations and innovators in the business press. It analyzes changes in the discourse of business journalism associated with the growth in business news and the development of new ways of framing business issues and events. Finally, it examines...... the organizational implications of the increased media visibility of business and, in particular, the development of corporate governance and media relations....

  19. Mediatized play

    DEFF Research Database (Denmark)

    Johansen, Stine Liv

    Children’s play must nowadays be understood as a mediatized field in society and culture. Media – understood in a very broad sense - holds severe explanatory power in describing and understanding the practice of play, since play happens both with, through and inspired by media of different sorts....... In this presentation the case of ‘playing soccer’ will be outlined through its different mediated manifestations, including soccer games and programs on TV, computer games, magazines, books, YouTube videos and soccer trading cards....

  20. mediation: R Package for Causal Mediation Analysis

    Directory of Open Access Journals (Sweden)

    Dustin Tingley

    2014-09-01

    Full Text Available In this paper, we describe the R package mediation for conducting causal mediation analysis in applied empirical research. In many scientific disciplines, the goal of researchers is not only estimating causal effects of a treatment but also understanding the process in which the treatment causally affects the outcome. Causal mediation analysis is frequently used to assess potential causal mechanisms. The mediation package implements a comprehensive suite of statistical tools for conducting such an analysis. The package is organized into two distinct approaches. Using the model-based approach, researchers can estimate causal mediation effects and conduct sensitivity analysis under the standard research design. Furthermore, the design-based approach provides several analysis tools that are applicable under different experimental designs. This approach requires weaker assumptions than the model-based approach. We also implement a statistical method for dealing with multiple (causally dependent mediators, which are often encountered in practice. Finally, the package also offers a methodology for assessing causal mediation in the presence of treatment noncompliance, a common problem in randomized trials.

  1. Bayesian Mediation Analysis

    Science.gov (United States)

    Yuan, Ying; MacKinnon, David P.

    2009-01-01

    In this article, we propose Bayesian analysis of mediation effects. Compared with conventional frequentist mediation analysis, the Bayesian approach has several advantages. First, it allows researchers to incorporate prior information into the mediation analysis, thus potentially improving the efficiency of estimates. Second, under the Bayesian…

  2. Mediation as Signal

    NARCIS (Netherlands)

    Holler, M.J.; Lindner, I.

    2004-01-01

    This paper analyzes mediation as a signal. Starting from a stylized case, a game theoretical model of one-sided incomplete information, taken from Cho and Kreps (1987), is applied to discuss strategic effects of mediation. It turns out that to reject mediation can be interpreted as a ”negative signa

  3. Technology-Use Mediation

    DEFF Research Database (Denmark)

    Bansler, Jørgen P.; Havn, Erling C.

    2003-01-01

    This study analyzes how a group of ‘mediators’ in a large, multinational company adapted a computer-mediated communication technology (a ‘virtual workspace’) to the organizational context (and vice versa) by modifying features of the technology, providing ongoing support for users, and promoting...... of technology-use mediation is more complex and indeterminate than earlier literature suggests. In particular, we want to draw attention to the fact that advanced computer-mediated communication technologies are equivocal and that technology-use mediation consequently requires ongoing sensemaking (Weick 1995)....... appropriate conventions of use. Our findings corroborate earlier research on technology-use mediation, which suggests that such mediators can exert considerable influence on how a particular technology will be established and used in an organization. However, this study also indicates that the process...

  4. Dynamic public service mediation

    NARCIS (Netherlands)

    Hofman, W.; Staalduinen, M. van

    2010-01-01

    This paper presents an approach to dynamic public service mediation. It is based on a conceptual model and the use of search and ranking algorithms. The conceptual model is based on Abstract State Machine theory. Requirements for dynamic service mediation were derived from a real-world case. The con

  5. Teaching Mediated Public Relations.

    Science.gov (United States)

    Kent, Michael L.

    2001-01-01

    Discusses approaches to teaching a mediated public relations course, emphasizing the World Wide Web. Outlines five course objectives, assignments and activities, evaluation, texts, and lecture topics. Argues that students mastering these course objectives will understand ethical issues relating to media use, using mediated technology in public…

  6. Ruimte voor mediation

    NARCIS (Netherlands)

    Combrink-Kuiters, L.; Niemeijer, E.; Voert, M. ter

    2003-01-01

    Dit onderzoek is samen met ADR en mediation Rechterlijke macht gepubliceerd. Het doel was enerzijds na te gaan of, en onder welke condities, mediation in de Nederlandse context een effectief en efficiënt alternatief is voor de gerechtelijke geschillenbeslechting. Anderzijds inzicht te verwerven in d

  7. Music, Radio, and Mediatization

    DEFF Research Database (Denmark)

    Krogh, Mads; Michelsen, Morten

    2016-01-01

    Mediatization has become a key concept for understanding the relations between media and other cultural and social fields. Contributing to the discussions related to the concept of mediatization, this article discusses how practices of radio and music(al life) influence each other. We follow Deacon......’s and Stanyer’s advice to supplement the concept of mediatization with ‘a series of additional concepts at lower levels of abstraction’ and suggest, in this respect, the notion of heterogeneous milieus of music– radio. Hereby, we turn away from the all-encompassing perspectives related to the concept...... of mediatization where media as such seem to be ascribed agency. Instead, we consider historical accounts of music–radio in order to address the complex non- linearity of concrete processes of mediatization as they take place in the multiple meetings between a decentred notion of radio and musical life....

  8. The Schizosaccharomyces pombe Mediator

    DEFF Research Database (Denmark)

    Venturi, Michela

    In the past several years great attention has been dedicated to the characterization of the Mediator complex in a different range of model organisms. Mediator is a conserved co-activator complex involved in transcriptional regulation and it conveys signals from regulatory transcription factors......+ is a nonessential gene, while deletion of med11+ resulted in unviable cells. These results are in line with those obtained in S. cerevisiae. Isolation of S. pombe Mediator by the tandem affinity purification method and Co-IP experiments lead to the conclusion that Med9 and Med11 might not belong to the Mediator...... complex, but our results did not exclude it completely either. Our attempts to demonstrate the presence of these two subunits in the Mediator complex remain inconclusive primarily due to the lack of proper expression of the tagged versions of the proteins. However, we have paved a way to further...

  9. [Mediation in health].

    Science.gov (United States)

    Decastello, Alice

    2008-02-10

    The author presents mediation as an alternative dispute resolution method. Mediation is a process where the parties are ready to settle their dispute out of court, by way of negotiation and with the involvement of an independent third person as mediator. In the mediation process the mediator shall not decide the dispute, nor examine the default or give legal advice or express his/her opinion - the mediator's duty is to help the parties bring their positions closer and come to a settlement agreement within a short time (120 days). The author gives a summary of the applications of the Hungarian Act on Mediation in Public Health and draws conclusions from the practical experience since entry into force of the legislation and illustrates the advantages of mediation over the court procedure (which may drag on for years). The primary advantages of mediation are that both the mediators and the parties are bound by the obligation of secrecy, the procedure is cheaper than the court proceedings, and the parties can "save their faces" because in mediation the winner-winner formula asserts itself - against lawsuits where the winner-loser positions are confronted. The author also analyses the specific data and information available so far. As for the future, the legislation needs to be amended at several points. It is particularly expedient to regulate the legal relationship between the insurance companies and the health service providers because the liability insurance may not cover the damages the courts adjudicate. And so some of the service providers may go bankrupt as the difference in excess of the upper limit of coverage - it might as well be up to HUF 5 million per case - shall be paid from own budget, to the charge of the upkeep costs. It is also required to review and amend the regulations on expert activities, just as it is inevitable to make data supply compulsory - otherwise it will be impossible to monitor the number of mediation procedures in health. At present

  10. General resonance mediation

    Energy Technology Data Exchange (ETDEWEB)

    McGarrie, Moritz

    2012-07-15

    We extend the framework of general gauge mediation to cases where the mediating fields have a nontrivial spectral function, as might arise from strong dynamics. We demonstrate through examples that this setup describes a broad class of possible models of gauge mediated supersymmetry breaking. A main emphasis is to give general formulas for cross sections for {sigma}(visible {yields} hidden) in these resonance models. We will also give formulas for soft masses, A-terms and demonstrate the framework with a holographic setup.

  11. Technology-Use Mediation

    DEFF Research Database (Denmark)

    Bansler, Jørgen P.; Havn, Erling C.

    2004-01-01

    Implementation of new computer-mediated communication (CMC) systems in organizations is a complex socio-technical endeavour, involving the mutual adaptation of technology and organization over time. Drawing on the analytic concept of sensemaking, this paper provides a theoretical perspective...... that deepens our understanding of how organizations appropriate new electronic communication media. The paper analyzes how a group of mediators in a large, multinational company adapted a new web-based CMC technology (a virtual workspace) to the local organizational context (and vice versa) by modifying...... features of the technology, providing ongoing support for users, and promoting appropriate conventions of use. We found that these mediators exerted considerable influence on how the technology was established and used in the organization. The mediators were not neutral facilitators of a well...

  12. Natural generalized mirage mediation

    CERN Document Server

    Baer, Howard; Serce, Hasan; Tata, Xerxes

    2016-01-01

    In the supersymmetric scenario known as mirage mediation (MM), the soft SUSY breaking terms receive comparable anomaly-mediation and moduli-mediation contributions leading to the phenomenon of mirage unification. The simplest MM SUSY breaking models which are consistent with the measured Higgs mass and sparticle mass constraints are strongly disfavoured by fine-tuning considerations. However, while MM makes robust predictions for gaugino masses, the scalar sector is quite sensitive to specific mechanisms for moduli stabilization and potential uplifting. We suggest here a broader setup of generalized mirage mediation (GMM), where heretofore discrete parameters are allowed as continuous to better parametrize these other schemes. We find that natural SUSY spectra consistent with both the measured value of m(h). as well as LHC lower bounds on superpartner masses are then possible. We explicitly show that models generated from natural GMM may be beyond the reach of even high-luminosity LHC searches. In such a case...

  13. DREDed Anomaly Mediation

    CERN Document Server

    Boyda, E; Pierce, A T; Boyda, Ed; Murayama, Hitoshi; Pierce, Aaron

    2002-01-01

    We offer a guide to dimensional reduction (DRED) in theories with anomaly mediated supersymmetry breaking. Evanescent operators proportional to epsilon arise in the bare Lagrangian when it is reduced from d=4 to d= (4-2 epsilon) dimensions. In the course of a detailed diagrammatic calculation, we show that inclusion of these operators is crucial. The evanescent operators conspire to drive the supersymmetry-breaking parameters along anomaly-mediation trajectories across heavy particle thresholds, guaranteeing the ultraviolet insensitivity.

  14. Metadata based mediator generation

    Energy Technology Data Exchange (ETDEWEB)

    Critchlow, T

    1998-03-01

    Mediators are a critical component of any data warehouse, particularly one utilizing partially materialized views; they transform data from its source format to the warehouse representation while resolving semantic and syntactic conflicts. The close relationship between mediators and databases, requires a mediator to be updated whenever an associated schema is modified. This maintenance may be a significant undertaking if a warehouse integrates several dynamic data sources. However, failure to quickly perform these updates significantly reduces the reliability of the warehouse because queries do not have access to the m current data. This may result in incorrect or misleading responses, and reduce user confidence in the warehouse. This paper describes a metadata framework, and associated software designed to automate a significant portion of the mediator generation task and thereby reduce the effort involved in adapting the schema changes. By allowing the DBA to concentrate on identifying the modifications at a high level, instead of reprogramming the mediator, turnaround time is reduced and warehouse reliability is improved.

  15. Fashion, Mediations & Method Assemblages

    DEFF Research Database (Denmark)

    Sommerlund, Julie; Jespersen, Astrid Pernille

    of handling multiple, fluid realities with multiple, fluid methods. Empirically, the paper works with mediation in fashion - that is efforts the active shaping of relations between producer and consumer through communication, marketing and PR. Fashion mediation is by no means simple, but organise complex...... Modern. New York, Harvester-Wheatsheaf. Law, J. (2004). After Method - mess in social science research. London and New York, Routledge......., it is an important ambition of this paper to go into a methodological discussion of how "that which effectively happens" can be approached. To this end, the paper will combine Hennion's term of the "mediator" with John Laws methodological term of "method assemblages". Method assemblages is a suggested as a way...

  16. The Strategic Mediator

    DEFF Research Database (Denmark)

    Rossignoli, Cecilia; Carugati, Andrea; Mola, Lapo

    2009-01-01

    The last 10 years have witnessed the emergence of electronic marketplaces as players that leverage new technologies to facilitate B2B internet-mediated collaborative business. Nowadays these players are augmenting their services from simple intermediation to include new inter-organizational relat......The last 10 years have witnessed the emergence of electronic marketplaces as players that leverage new technologies to facilitate B2B internet-mediated collaborative business. Nowadays these players are augmenting their services from simple intermediation to include new inter......-marketplace assumes the paradoxical role of strategic mediator: an agent who upholds and heightens the fences of the transactions instead of leveling them. The results have implication in shaping how we see the role of technology as strategic or commoditized....

  17. Models as Mediators

    DEFF Research Database (Denmark)

    Sommerlund, Julie

    from laboratory studies, (Latour 1979; Lynch 1985; Sommerlund 2004 (2007); Sommerlund 2006) and is complemented by the attention paid to the "mediator" by Hennion (1989; 1997; 2005). The empirical focus will be on a central - but overlooked - actor of branding and advertising; the model. The model has...... solely been theorized within cultural studies (Craik 1994) as feminine spectacle, but has been neglected as mediator and actor. This paper will argue that models are co-producers of brands, and vice versa. Empirically, the paper will present interviews with models, model-scouts, agents, and advertisers...

  18. Mediated intimacy in families

    DEFF Research Database (Denmark)

    Stougaard, Malthe Kirkhoff

    2006-01-01

    Mediating intimacy between children and their parents is still limited investigated and at the same time, we find that, emerging technologies are about to change and affect the way we interact with each other. In this paper, we report from an empirical study where we investigated the social...... with other types of intimate relations such as strong-tie intimacy (couples cohabiting). However, we also identified several issues of intimacy unique to the special relation between children and their parents. These unique acts of intimacy propose challenges when designing technologies for mediated intimacy...

  19. Teachers as mediators

    DEFF Research Database (Denmark)

    Dorf, Hans; Kelly, Peter; Hohmann, Ulrike

    2012-01-01

    Within the context of lower secondary English teaching in South West England, this study identifies in broad terms the competing goals between which English teachers mediate and the explicit and hidden tensions that result. To understand the interactions of competing goals, teachers’ goal...

  20. Mediatization and Government Communication

    DEFF Research Database (Denmark)

    Laursen, Bo; Valentini, Chiara

    2015-01-01

    in the light of mediatization and government communication theories. Without one pan-European public sphere, the European Parliament, like the other European Union (EU) institutions, competes with national actors for the news media’s attention in the EU’s twenty-eight national public spheres, where EU affairs...

  1. Expanding mediation theory

    NARCIS (Netherlands)

    Verbeek, P.P.C.C.

    2012-01-01

    In his article In Between Us, Yoni van den Eede expands existing theories of mediation into the realm of the social and the political, focusing on the notions of opacity and transparency. His approach is rich and promising, but two pitfalls should be avoided. First, his concept of ‘in-between’ runs

  2. Mediation and Automatization.

    Science.gov (United States)

    Hutchins, Edwin

    This paper discusses the relationship between the mediation of task performance by some structure that is not inherent in the task domain itself and the phenomenon of automatization, in which skilled performance becomes effortless or phenomenologically "automatic" after extensive practice. The use of a common simple explicit mediating…

  3. Thermally favourable gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Dalianis, Ioannis, E-mail: Ioannis.Dalianis@fuw.edu.p [Institute of Theoretical Physics, Faculty of Physics, University of Warsaw, ul. Hoza 69, Warsaw (Poland); Lalak, Zygmunt, E-mail: Zygmunt.Lalak@fuw.edu.p [Institute of Theoretical Physics, Faculty of Physics, University of Warsaw, ul. Hoza 69, Warsaw (Poland)

    2011-03-14

    We discuss the thermal evolution of the spurion and messenger fields of ordinary gauge mediation models taking into account the Standard Model degrees of freedom. It is shown that for thermalized messengers the metastable susy breaking vacuum becomes thermally selected provided that the susy breaking sector is sufficiently weakly coupled to messengers or to any other observable field.

  4. Natural generalized mirage mediation

    Science.gov (United States)

    Baer, Howard; Barger, Vernon; Serce, Hasan; Tata, Xerxes

    2016-12-01

    In the supersymmetric scenario known as mirage mediation (MM), the soft supersymmetry (SUSY) breaking terms receive comparable anomaly-mediation and moduli-mediation contributions leading to the phenomenon of mirage unification. The simplest MM SUSY breaking models which are consistent with the measured Higgs mass and sparticle mass constraints are strongly disfavored by fine-tuning considerations. However, while MM makes robust predictions for gaugino masses, the scalar sector is quite sensitive to specific mechanisms for moduli stabilization and potential uplifting. We suggest here a broader setup of generalized mirage mediation (GMM), where heretofore discrete parameters are allowed as continuous to better parametrize these other schemes. We find that natural SUSY spectra consistent with both the measured value of mh as well as LHC lower bounds on superpartner masses are then possible. We explicitly show that models generated from natural GMM may be beyond the reach of even high-luminosity LHC searches. In such a case, the proposed International Linear e+e- Collider will be required for natural SUSY discovery via higgsino pair production reactions. We also outline prospects for detection of higgsino-like WIMPs from natural GMM.

  5. Nanofluids mediating surface forces.

    Science.gov (United States)

    Pilkington, Georgia A; Briscoe, Wuge H

    2012-11-01

    Fluids containing nanostructures, known as nanofluids, are increasingly found in a wide array of applications due to their unique physical properties as compared with their base fluids and larger colloidal suspensions. With several tuneable parameters such as the size, shape and surface chemistry of nanostructures, as well as numerous base fluids available, nanofluids also offer a new paradigm for mediating surface forces. Other properties such as local surface plasmon resonance and size dependent magnetism of nanostructures also present novel mechanisms for imparting tuneable surface interactions. However, our fundamental understanding, experimentally and theoretically, of how these parameters might affect surface forces remains incomplete. Here we review recent results on equilibrium and dynamic surface forces between macroscopic surfaces in nanofluids, highlighting the overriding trends in the correlation between the physical parameters that characterise nanofluids and the surface forces they mediate. We also discuss the challenges that confront existing surface force knowledge as a result of this new paradigm.

  6. The mediatization of journalism

    Directory of Open Access Journals (Sweden)

    Aske Kammer

    2014-06-01

    Full Text Available Proposing an explanation of current macro-sociological changes and institutional transformations in journalism, this article argues that journalism is currently undergoing a process of mediatization. Drawing upon the international research literature as well as statements from interviews with news workers working on Danish news websites, the article examines four current trends in journalism that are closely connected to the rise of news on the web, namely the use of the affordances of news websites, radical commercialization, increased audience participation in news production, and the increased multi-skilling and simultaneous de-skilling of journalists. Taken together, these trends reflect a process through which journalism increasingly subsumes itself to the logic of the media, suggesting mediatization as an adequate explanatory framework. One implication of such a process is that journalism seems to be transforming from an occupational profession into an organizational one.

  7. [Healthcare mediation model for nerologists].

    Science.gov (United States)

    Ando, Tetsuo

    2011-11-01

    Mediation offers a process by which two parties work towards an agreement with the aid of a neutral third party. Physicians and nurses can apply healthcare mediation model to ordinary medical practice for preventing conflict. Communication using mediation skills improves patient-physician relationship, and prevents medical malpractice and conflict.

  8. Surface Mediated Photocatalysis.

    Science.gov (United States)

    1987-12-01

    Bipolar Photoelectrodes," M.A. Fox, Nouv. J. Chim. 1987, II, 129. 4. "Effect of Cosolvent Additives on Relative Rates of Photooxidation on...Semiconductor Surfaces," D.D. Sackett, M.A. Fox, J. Phys. Org. Chem, 19_8, in press. 5. "Selectivity in the Semiconductor-Mediated Photooxidation of Polyols ...surfaced properties of the heteropolytungstates were varied. The conceptual mechanistic picture involved in the heteropolyoxo induced photooxidations

  9. What carries a mediation process? Configural analysis of mediation.

    Science.gov (United States)

    von Eye, Alexander; Mun, Eun Young; Mair, Patrick

    2009-09-01

    Mediation is a process that links a predictor and a criterion via a mediator variable. Mediation can be full or partial. This well-established definition operates at the level of variables even if they are categorical. In this article, two new approaches to the analysis of mediation are proposed. Both of these approaches focus on the analysis of categorical variables. The first involves mediation analysis at the level of configurations instead of variables. Thus, mediation can be incorporated into the arsenal of methods of analysis for person-oriented research. Second, it is proposed that Configural Frequency Analysis (CFA) can be used for both exploration and confirmation of mediation relationships among categorical variables. The implications of using CFA are first that mediation hypotheses can be tested at the level of individual configurations instead of variables. Second, this approach leaves the door open for different types of mediation processes to exist within the same set. Using a data example, it is illustrated that aggregate-level analysis can overlook mediation processes that operate at the level of individual configurations.

  10. Urban Songlines as Mediator

    DEFF Research Database (Denmark)

    Corlin, Anne

    2016-01-01

    The aim of this paper is to present an investigation of the method The Urban Songlines Book and how it works as a mediator for mapping the experienced space. The method contains a combination of aerial maps, photographs, and interviews as a way to understand the respondent´s use, relations...... and experiences of their neighborhood and the city. Through a presentation of the origin of the method, a description of the conducted study, and an analysis of the process in relation to theories about participatory design, social design, ANT and architectural sociology, the paper reveals how this method...

  11. Sociocultural mediators of remembering

    DEFF Research Database (Denmark)

    Wagoner, Brady; Gillespie, Alex

    2014-01-01

    , questioning and deferring contribute to the transformation and conventionalization of the material. These diverse sociocultural mediators are integrated into a partially coherent recollection by participants self-reflecting, or as Bartlett termed it, turning around upon their schemas. We demonstrate...... that this self-reflection is both a social and a psychological process, occurring because participants are responding to their own utterances in the same way that they respond to the utterances of other people. These empirical findings are used to make a case for using discursive data to look not only...

  12. Mediatization and Government Communication

    DEFF Research Database (Denmark)

    Laursen, Bo; Valentini, Chiara

    2015-01-01

    in the light of mediatization and government communication theories. Without one pan-European public sphere, the European Parliament, like the other European Union (EU) institutions, competes with national actors for the news media’s attention in the EU’s twenty-eight national public spheres, where EU affairs......, and particularly into the thinking that guides the efforts of these European Parliament officials to increase European citizens’ awareness of, and support for, the European Parliament that is meant to voice the citizens’ concerns in political processes at the EU level....

  13. Computer Mediated Communication

    Science.gov (United States)

    Fano, Robert M.

    1984-08-01

    The use of computers in organizations is discussed in terms of its present and potential role in facilitating and mediating communication between people. This approach clarifies the impact that computers may have on the operation of organizations and on the individuals comprising them. Communication, which is essential to collaborative activities, must be properly controlled to protect individual and group privacy, which is equally essential. Our understanding of the human and organizational aspects of controlling communication and access to information presently lags behind our technical ability to implement the controls that may be needed.

  14. Holographic Gauge Mediation

    Energy Technology Data Exchange (ETDEWEB)

    Benini, Francesco; /Princeton U.; Dymarsky, Anatoly; /Stanford U., ITP; Franco, Sebastian; /Santa Barbara, KITP; Kachru, Shamit; Simic, Dusan; /Stanford U., ITP /SLAC; Verlinde, Herman; /Princeton, Inst. Advanced Study

    2009-06-19

    We discuss gravitational backgrounds where supersymmetry is broken at the end of a warped throat, and the SUSY-breaking is transmitted to the Standard Model via gauginos which live in (part of) the bulk of the throat geometry. We find that the leading effect arises from splittings of certain 'messenger mesons,' which are adjoint KK-modes of the D-branes supporting the Standard Model gauge group. This picture is a gravity dual of a strongly coupled field theory where SUSY is broken in a hidden sector and transmitted to the Standard Model via a relative of semi-direct gauge mediation.

  15. Surface-Mediated Photocatalysis

    Science.gov (United States)

    1992-02-04

    Photocatalysis 6. AUTHOR(S) DIMarye Anne Fox E -LECTE ’ / 7. PERFORMING ORGANIZATION NAME(S) AND AD ES). 8. PERFORMING ORGANIZATION REPORT NUMBER University of...Form 298 (Rev 2-89) Prescr d bv ANJ std Z39.1s 291-102 j Final Report on Surface-Mediated Photocatalysis "a,. . ARO Proposal No. 28298-CH u - Work from...Produced by Anodic Oxidation and by Photoelectrochemical Activation of TiO2 ," Marye Anne Fox and Karl L. Worthen, Chem. Mater. 1991, 3, 253. "Surface

  16. 45 CFR 16.18 - Mediation.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Mediation. 16.18 Section 16.18 Public Welfare... BOARD § 16.18 Mediation. (a) In cases pending before the Board. If the Board decides that mediation... mediation techniques and will provide or assist in selecting a mediator. The mediator may take any...

  17. Mediation Revisited: The Interactive Organization of Mediation in Learning Environments

    OpenAIRE

    Pekarek Doehler, Simona

    2013-01-01

    This article is concerned with the social organization of mediation in learning environments. It seeks to further articulate the sociocultural notion of mediation in sociointeractional terms, combining insights from the sociocultural approach to cognition and the microinteractionist, especially ethnomethodological approach to social activities. A microanalysis of mediation in communicative 2nd-language classroom activities where the task at hand is the management of interaction itself is pres...

  18. Magnetically mediated thermoacoustic imaging

    Science.gov (United States)

    Feng, Xiaohua; Gao, Fei; Zheng, Yuanjin

    2014-03-01

    In this paper, alternating magnetic field is explored for inducing thermoacoustic effect on dielectric objects. Termed as magnetically mediated thermo-acoustic (MMTA) effect that provides a contrast in conductivity, this approach employs magnetic resonance for delivering energy to a desired location by applying a large transient current at radio frequency below 50MHz to a compact magnetically resonant coil. The alternating magnetic field induces large electric field inside conductive objects, which then undergoes joule heating and emanates acoustic signal thermo-elastically. The magnetic mediation approach with low radio frequency can potentially provide deeper penetration than microwave radiation due to the non-magnetic nature of human body and therefore extend thermoacoustic imaging to deep laid organs. Both incoherent time domain method that applies a pulsed radio frequency current and coherent frequency domain approach that employs a linear chirp signal to modulate the envelop of the current are discussed. Owing to the coherent processing nature, the latter approach is capable of achieving much better signal to noise ratio and therefore potential for portable imaging system. Phantom experiments are carried out to demonstrate the signal generation together with some preliminary imaging results. Ex-vivo tissue studies are also investigated.

  19. Mediating Trust in Terrorism Coverage

    DEFF Research Database (Denmark)

    Mogensen, Kirsten

    crisis. While the framework is presented in the context of television coverage of a terror-related crisis situation, it can equally be used in connection with all other forms of mediated trust. Key words: National crisis, risk communication, crisis management, television coverage, mediated trust.......Mass mediated risk communication can contribute to perceptions of threats and fear of “others” and/or to perceptions of trust in fellow citizens and society to overcome problems. This paper outlines a cross-disciplinary holistic framework for research in mediated trust building during an acute...

  20. When Memories are Mediated

    DEFF Research Database (Denmark)

    Frølunde, Lisbeth; Bjerregaard, Mette

    2013-01-01

    and political contexts and media platforms take place and become contexts for audience reception. This paper explores two examples of narratives that construct memories of acts of mass violence: “Gzim Rewind” (Sweden, 2011, director Knutte Wester) about 1990’s Kosovo, and “The Act of Killing” (Denmark, 2012......Acts of mass violence, including murder on civilians, genocide, oppression and wars, can mobilize memories of the involved persons and following generations in a certain historical situation. Acts of mass violence can also create a sort of looking glass of culturally dominant memories...... that are mediated through stories: told and retold as oral stories through generations, as myths or sagas, or remediated as contemporary documentary film accounts or more fictional film accounts. In these processes of retelling acts of violence, transformations of meanings across time, cultural, social...

  1. Neutrino assisted gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyung Do; Mo, Doh Young; Seo, Min-Seok [Seoul National University, Department of Physics and Astronomy and Center for Theoretical Physics, Seoul (Korea, Republic of)

    2013-06-15

    Recent observation shows that the Higgs mass is at around 125 GeV while the prediction of the minimal supersymmetric standard model is below 120 GeV for stop mass lighter than 2 TeV unless the top squark has a maximal mixing. We consider the right-handed neutrino supermultiplets as messengers in addition to the usual gauge mediation to obtain sizeable trilinear soft parameters A{sub t} needed for the maximal stop mixing. Neutrino messengers can explain the observed Higgs mass for stop mass around 1 TeV. Neutrino assistance can also generate charged lepton flavor violation including {mu}{yields}e {gamma} as a possible signature of the neutrino messengers. We consider the S{sub 4} discrete flavor model and show the relation of the charged lepton flavor violation, {theta} {sub 13} of neutrino oscillation and the muon's g-2. (orig.)

  2. The mediation procedure in Romania

    Directory of Open Access Journals (Sweden)

    Alexandrina Zaharia

    2009-06-01

    Full Text Available The mediation activity as an alternative way of solving conflicts occupies an important place in modernsociety. Currently, the mediation reached its maturity worldwide being adopted without reservations.The future of solving conflicts is undoubtedly closely related to mediation. XXth century is the century of solvingconflicts amiably outside the court room. In Romania and the mediation profession were regulated by the Law no.192/2006, on the basis of the idea that mediation is one of the major themes of the reform strategy of the judicialsystem 2005-2007. By adopting the mentioned law it was followed the idea of reducing the volume of activitycourts, and therefore, relieve them of as many cases, with the direct effect on the quality of justice. Mediation is avoluntary process in which the parties with a neutral and impartial third party, without power of decision - themediator - who is qualified to assist the parties to negotiate, facilitating the communication between them andhelping them to reach a unanimous effective and sustainable agreement. The parties may resort to mediation beforeor after triggering a trial. Mediation can be applied, in principle, on any type of conflict. However, theRomanian legislator has established special stipulations on conflict mediation in criminal, civil and familylaw. Although not expressly provided, the stipulations regarding the civil conflicts and also apply to commercialconflicts. Therefore, the mediation is applicable to most types of lawsuits, except those relating to personalrights. As a "win- win" principle, the mediation does not convert any of the parties defeated or victorious; allthose involved have gained by applying this procedure.

  3. Theorizing with/out "Mediators".

    Science.gov (United States)

    Roth, Wolff-Michael; Jornet, Alfredo

    2017-01-05

    Mediation is one of the most often cited concepts in current cultural-historical theory literature, in which cultural actions and artifacts are often characterized as mediators standing between situational stimuli and behavioral responses. Most often presented as a means to overcome Cartesian dualism between subject and object, and between individual and society, some scholars have nonetheless raised criticism suggesting that such mediators are problematic for a dialectical psychology that takes a unit analysis (monist) approach. In fact, Spinoza develops a monist theory of mind and body that goes without and even excludes every form of mediation. In this study, we follow up on the latter criticisms and explore what we consider to be problematic uses of the notion of mediation as an analytical construct in the literature. We elaborate an empirically grounded discussion on the ways the concept of mediation may lead to dualistic readings; and we offer an alternative account where the notion of mediator is not needed. We conclude discussing prospects for and implications of a cultural-historical theory where the notion of mediation no longer is invoked to account for human action and development.

  4. Anomaly mediation deformed by axion

    Energy Technology Data Exchange (ETDEWEB)

    Nakayama, Kazunori, E-mail: kazunori@hep-th.phys.s.u-tokyo.ac.jp [Department of Physics, University of Tokyo, Bunkyo-ku, Tokyo 113-0033 (Japan); Kavli Institute for the Physics and Mathematics of the Universe, University of Tokyo, Kashiwa 277-8583 (Japan); Yanagida, Tsutomu T. [Kavli Institute for the Physics and Mathematics of the Universe, University of Tokyo, Kashiwa 277-8583 (Japan)

    2013-05-13

    We show that in supersymmetric axion models the axion supermultiplet obtains a sizable F-term due to a non-supersymmetric dynamics and it generally gives the gaugino masses comparable to the anomaly mediation contribution. Thus the gaugino mass relation predicted by the anomaly mediation effect can be significantly modified in the presence of axion to solve the strong CP problem.

  5. Practical Guide to Civil Mediation

    CERN Multimedia

    2006-01-01

    The Permanent Mission of Switzerland has informed CERN that the Département des Institutions of the Republic and Canton of Geneva and the Groupement suisse des Magistrats pour la médiation (GEMME) - Swiss Association of Magistrates for Mediation have published a multilingual Practical Guide to Civil Mediation (including English). In this context, the Swiss Mission has underlined the benefits of resorting to mediation, especially for the personnel of International Organizations, and which the Secretary-General of the GEMME has summarised as follows: it is a private process not requiring the waiver of the parties' immunities; the confidentiality of the mediation process is guaranteed both by the mediator and the parties to it; the search for an amicable settlement does not need to be determined by reference to law (provided that public order is respected); the process is faster (2 to 3 sessions), less costly and more flexible than civil or arbitration procedures; in order to reinforce the agreem...

  6. Practical Guide to Civil Mediation

    CERN Multimedia

    2006-01-01

    The Permanent Mission of Switzerland has informed CERN that the Département des Institutions of the Republic and Canton of Geneva and the Groupement suisse des Magistrats pour la médiation (GEMME) - Swiss Association of Magistrates for Mediation have published a multilingual Practical Guide to Civil Mediation (including English). In this context, the Swiss Mission has underlined the benefits of resorting to mediation, especially for the personnel of international organizations, and which the Secretary-General of the GEMME has summarised as follows: it is a private process not requiring the waiver of the parties' immunities; the confidentiality of the mediation process is guaranteed both by the mediator and the parties to it; the search for an amicable settlement does not need to be determined by reference to law (provided that public order is respected); the process is faster (2 to 3 sessions), less costly and more flexible than civil or arbitration procedures; in order to reinforce the agreeme...

  7. Microbially mediated mineral carbonation

    Science.gov (United States)

    Power, I. M.; Wilson, S. A.; Dipple, G. M.; Southam, G.

    2010-12-01

    Mineral carbonation involves silicate dissolution and carbonate precipitation, which are both natural processes that microorganisms are able to mediate in near surface environments (Ferris et al., 1994; Eq. 1). (Ca,Mg)SiO3 + 2H2CO3 + H2O → (Ca,Mg)CO3 + H2O + H4SiO4 + O2 (1) Cyanobacteria are photoautotrophs with cell surface characteristics and metabolic processes involving inorganic carbon that can induce carbonate precipitation. This occurs partly by concentrating cations within their net-negative cell envelope and through the alkalinization of their microenvironment (Thompson & Ferris, 1990). Regions with mafic and ultramafic bedrock, such as near Atlin, British Columbia, Canada, represent the best potential sources of feedstocks for mineral carbonation. The hydromagnesite playas near Atlin are a natural biogeochemical model for the carbonation of magnesium silicate minerals (Power et al., 2009). Field-based studies at Atlin and corroborating laboratory experiments demonstrate the ability of a microbial consortium dominated by filamentous cyanobacteria to induce the precipitation of carbonate minerals. Phototrophic microbes, such as cyanobacteria, have been proposed as a means for producing biodiesel and other value added products because of their efficiency as solar collectors and low requirement for valuable, cultivable land in comparison to crops (Dismukes et al., 2008). Carbonate precipitation and biomass production could be facilitated using specifically designed ponds to collect waters rich in dissolved cations (e.g., Mg2+ and Ca2+), which would allow for evapoconcentration and provide an appropriate environment for growth of cyanobacteria. Microbially mediated carbonate precipitation does not require large quantities of energy or chemicals needed for industrial systems that have been proposed for rapid carbon capture and storage via mineral carbonation (e.g., Lackner et al., 1995). Therefore, this biogeochemical approach may represent a readily

  8. 29 CFR 1203.1 - Mediation services.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Mediation services. 1203.1 Section 1203.1 Labor Regulations Relating to Labor (Continued) NATIONAL MEDIATION BOARD APPLICATIONS FOR SERVICE § 1203.1 Mediation services. Applications for the mediation services of the National Mediation Board under section 5, First, of the...

  9. 29 CFR 1202.1 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Mediation. 1202.1 Section 1202.1 Labor Regulations Relating to Labor (Continued) NATIONAL MEDIATION BOARD RULES OF PROCEDURE § 1202.1 Mediation. The mediation..., or where conferences are refused. The National Mediation Board may proffer its services in case...

  10. 22 CFR 143.33 - Mediation.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Mediation. 143.33 Section 143.33 Foreign... Mediation. (a) Referral of complaints for mediation. The agency will refer to the Federal Mediation and... participate in the mediation process to the extent necessary to reach an agreement or make an...

  11. 34 CFR 81.13 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Mediation. 81.13 Section 81.13 Education Office of the... Mediation. (a) Voluntary mediation is available for proceedings that are pending before the OALJ. (b) A... mediation by filing a motion with the ALJ assigned to the case. The OALJ arranges for a mediator if...

  12. 24 CFR 3288.35 - Mediation.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 5 2010-04-01 2010-04-01 false Mediation. 3288.35 Section 3288.35...-Administered States § 3288.35 Mediation. (a) Mediator. The dispute resolution provider will provide for the... identifies any other party that should be included in the mediation, the mediator will contact the...

  13. 29 CFR 35.32 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Mediation. 35.32 Section 35.32 Labor Office of the Secretary... Mediation. (a) Referral to mediation. CRC will promptly refer to the Federal Mediation and Conciliation Service or the mediation agency designated by the Secretary of Health and Human Services under 45 CFR...

  14. 44 CFR 7.942 - Mediation.

    Science.gov (United States)

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Mediation. 7.942 Section 7..., Conciliation, and Enforcement Procedures § 7.942 Mediation. (a) FEMA will promptly refer to a mediation agency... participate in the mediation process to the extent necessary to reach an agreement or for the mediator to...

  15. 32 CFR 776.38 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 5 2010-07-01 2010-07-01 false Mediation. 776.38 Section 776.38 National... Professional Conduct § 776.38 Mediation. (a) Mediation: (1) A covered attorney may act as a mediator between... mediation, including the advantages and risks involved, and the effect on the...

  16. Thai and U.S. Community Mediation

    Science.gov (United States)

    Callister, Ronda Roberts; Wall, James A., Jr.

    2004-01-01

    The approaches of 111 Thai and a matched set of 111 U.S. community mediators are investigated. Results show that Thai mediators are more apt to be assertive in their mediations; they put disputants together, demand concessions, criticize disputants, and threaten them more frequently than do U.S. mediators. Thai mediators more frequently seek…

  17. Caspases: An apoptosis mediator

    Directory of Open Access Journals (Sweden)

    Tapan Kumar Palai

    2015-03-01

    Full Text Available The process of programmed cell death, or apoptosis, is generally characterized by distinct morphological characteristics and energy - dependent biochemical mechanisms. Apoptosis is a widely conserved phenomenon helping many processes, including normal cell turnover, proper development and functioning of the immune system, hormone dependent atrophy etc. Inappropriate apoptosis (either low level or high level leads to many developmental abnormalities like, neurodegenerative diseases, ischemic damage, autoimmune disorders and many types of cancer. To use cells for therapeutic purposes through generating cell lines, it is critical to study the cell cycle machinery and signalling pathways that controls cell death and apoptosis. Apoptotic pathways provide a fundamental protective mechanism that decreases cellular sensitivity to damaging events and allow proper developmental process in multi-cellular organisms. Major mediator of apoptosis is a family of proteins known as caspases. There are mainly fourteen types of caspases but out of them only ten caspasese have got essential role in controlling the process of apoptosis. These ten caspases have been categorized into either initiator caspases (caspase 2, 8, 9, 10 or executioner caspases (caspase 3, 6, 7. Although various types of caspases have been identified so far, the exact mechanisms of action of these groups of proteins is still to be fully understood. The aim of this review is to provide a detail overview of role of different caspases in regulating the process of apoptosis.

  18. Relativistic Radiation Mediated Shocks

    CERN Document Server

    Budnik, Ran; Sagiv, Amir; Waxman, Eli

    2010-01-01

    The structure of relativistic radiation mediated shocks (RRMS) propagating into a cold electron-proton plasma is calculated and analyzed. A qualitative discussion of the physics of relativistic and non relativistic shocks, including order of magnitude estimates for the relevant temperature and length scales, is presented. Detailed numerical solutions are derived for shock Lorentz factors $\\Gamma_u$ in the range $6\\le\\Gamma_u\\le30$, using a novel iteration technique solving the hydrodynamics and radiation transport equations (the protons, electrons and positrons are argued to be coupled by collective plasma processes and are treated as a fluid). The shock transition (deceleration) region, where the Lorentz factor $ \\Gamma $ drops from $ \\Gamma_u $ to $ \\sim 1 $, is characterized by high plasma temperatures $ T\\sim \\Gamma m_ec^2 $ and highly anisotropic radiation, with characteristic shock-frame energy of upstream and downstream going photons of a few~$\\times\\, m_ec^2$ and $\\sim \\Gamma^2 m_ec^2$, respectively.P...

  19. Anomaly Mediation and Cosmology

    CERN Document Server

    Basboll, A; Jones, D R T

    2011-01-01

    We consider an extension of the MSSM wherein anomaly mediation is the source of supersymmetry-breaking, and the tachyonic slepton problem is solved by a Fayet-Iliopoulos (FI) $D$-term associated with an additional $U(1)$ symmetry, which also facilitates the see-saw mechanism for neutrino masses and a natural source for the Higgs $\\mu$-term. We explore the cosmological consequences of the model, showing that the model naturally produces a period of hybrid inflation, terminating in the production of cosmic strings. In spite of the presence of a $U(1)$ with an FI term, inflation is effected by the $F$-term, with a $D$-flat tree potential (the FI term being cancelled by non-zero squark and slepton fields). Calculating the 1-loop corrections to the inflaton potential, we estimate the constraints on the parameters of the model from Cosmic Microwave Background data. We briefly discuss the mechanisms for baryogenesis via conventional leptogenesis, the out-of-equilibrium production of neutrinos from the cosmic strings...

  20. Mediation of information and educational mediation: conceptual discussions

    Directory of Open Access Journals (Sweden)

    Helena Célia de Souza Sacerdote

    2016-04-01

    Full Text Available Introduction: This is systematization of theoretical and methodological contributions related to the concepts of mediation information and pedagogical mediation in the literature. Objective: To understand possible intersection of information science and Online Education with regard to these concepts to check that both can be considered as analogous in its essence and practice. Methodology: Literature review based on literature by consulting the scientific productions selected in search of SciELO.ORG databases and EBSCO Host, the portal of CAPES / MEC and Google Scholar. Results: The most cited concepts in information science and education were de Almeida Junior (2009 and Masetto (2013, respectively. Conclusion: It is observed that the concept of mediation can move interchangeably between both areas. This is because the evidence found in the productions of the last five years indicate that the concept of information of mediation seems to have found its bases in education (educational psychology.

  1. In vivo and in situ measurement and modelling of intra-body effective complex permittivity

    DEFF Research Database (Denmark)

    Nadimi, Esmaeil S; Blanes-Vidal, Victoria; Harslund, Jakob L F

    2015-01-01

    Radio frequency tracking of medical micro-robots in minimally invasive medicine is usually investigated upon the assumption that the human body is a homogeneous propagation medium. In this Letter, the authors conducted various trial programs to measure and model the effective complex permittivity ε...... contractions and simulated peristaltic movements of the GI tract organs inside the abdominal cavity and in the presence of the abdominal wall on the measurements and variations of ε' and ε''. They advanced the previous models of effective complex permittivity of a multilayer inhomogeneous medium, by estimating...... an analytical model that accounts for reflections between the layers and calculates the attenuation that the wave encounters as it traverses the GI tract and the abdominal wall. They observed that deviation from the specified nominal layer thicknesses due to non-geometric boundaries of GI tract morphometric...

  2. Electrical characteristic of the titanium mesh electrode for transcutaneous intrabody communication to monitor implantable artificial organs.

    Science.gov (United States)

    Okamoto, Eiji; Kikuchi, Sakiko; Mitamura, Yoshinori

    2016-09-01

    We have developed a tissue-inducing electrode using titanium mesh to obtain mechanically and electrically stable contact with the tissue for a new transcutaneous communication system using the human body as a conductive medium. In this study, we investigated the electrical properties of the titanium mesh electrode by measuring electrode-tissue interface resistance in vivo. The titanium mesh electrode (Hi-Lex Co., Zellez, Hyogo, Japan) consisted of titanium fibers (diameter of 50 μm), and it has an average pore size of 200 μm and 87 % porosity. The titanium mesh electrode has a diameter of 5 mm and thickness of 1.5 mm. Three titanium mesh electrodes were implanted separately into the dorsal region of the rat. We measured the electrode-electrode impedance using an LCR meter for 12 weeks, and we calculated the tissue resistivity and electrode-tissue interface resistance. The electrode-tissue interface resistance of the titanium mesh electrode decreased slightly until the third POD and then continuously increased to 75 Ω. The electrode-tissue interface resistance of the titanium mesh electrode is stable and it has lower electrode-tissue interface resistance than that of a titanium disk electrode. The extracted titanium mesh electrode after 12 weeks implantation was fixed in 10 % buffered formalin solution and stained with hematoxylin-eosin. Light microscopic observation showed that the titanium mesh electrode was filled with connective tissue, inflammatory cells and fibroblasts with some capillaries in the pores of the titanium mesh. The results indicate that the titanium mesh electrode is a promising electrode for the new transcutaneous communication system.

  3. The simulation of electrostatic coupling intra-body communication based on the finite-element method

    Institute of Scientific and Technical Information of China (English)

    Song Yong; Zhang Kai; Yang Guang; Zhu Kang; Hao Qun

    2011-01-01

    In this paper, investigation has been done in the computer simulation of the electrostatic coupling IBC by using the developed finite-element models, in which a. the incidence and reflection of electronic signal in the upper arm model were analyzed by using the theory of electromagnetic wave; b. the finite-element models of electrostatic coupling IBC were developed by using the electromagnetic analysis package of ANSYS software; c. the signal attenuation of electrostatic coupling IBC were simulated under the conditions of different signal frequencies, electrodes directions, electrodes sizes and transmission distances. Finally, some important conclusions are deduced on the basis of simulation results.

  4. Assay of mast cell mediators

    DEFF Research Database (Denmark)

    Rådinger, Madeleine; Jensen, Bettina M; Swindle, Emily

    2015-01-01

    Mediator release from activated mast cells is a major initiator of the symptomology associated with allergic disorders such as anaphylaxis and asthma. Thus, methods to monitor the generation and release of such mediators have widespread applicability in studies designed to understand the processes...... regulating mast cell activation and for the identification of therapeutic approaches to block mast cell-driven disease. In this chapter, we discuss approaches used for the determination of mast cell degranulation, lipid-derived inflammatory mediator production, and cytokine/chemokine gene expression as well...

  5. Doing statistical mediation and moderation

    CERN Document Server

    Jose, Paul E

    2013-01-01

    Written in a friendly, conversational style, this book offers a hands-on approach to statistical mediation and moderation for both beginning researchers and those familiar with modeling. Starting with a gentle review of regression-based analysis, Paul Jose covers basic mediation and moderation techniques before moving on to advanced topics in multilevel modeling, structural equation modeling, and hybrid combinations, such as moderated mediation. User-friendly features include numerous graphs and carefully worked-through examples; ""Helpful Suggestions"" about procedures and pitfalls; ""Knowled

  6. The mediatization of ethical consumption

    Directory of Open Access Journals (Sweden)

    Mikkel Fugl Eskjær

    2013-03-01

    Full Text Available Over the years, mediatization studies have investigated the influence of media in numerous sections of contemporary society. One area that has received limited attention is the mediatization of consumption, particularly issues concerning ethical consumption. This article presents a study of how mediatization is transforming modern consumption and contributing to the mainstreaming of ethical consumption. Based on a study of a Danish online eco-store, the article argues that modern ethical consumption increasingly depends on new media practices to present sustainable consumption as practical and fashionable while effacing underlying processes of rationalisation and commercialisation.

  7. The mediatization of ethical consumption

    DEFF Research Database (Denmark)

    Eskjær, Mikkel Fugl

    2013-01-01

    Over the years, mediatization studies have investigated the influence of media in numerous sections of contemporary society. One area that has received limited attention is the mediatization of consumption, particularly issues concerning ethical consumption. This article presents a study of how...... mediatization is transforming modern consumption and contributing to the mainstreaming of ethical consumption. Based on a study of a Danish online eco-store, the article argues that modern ethical consumption increasingly depends on new media practices to present sustainable consumption as practical...

  8. Gauge Mediation in String Theory

    OpenAIRE

    Kawano, Teruhiko; Ooguri, Hirosi; Ookouchi, Yutaka

    2007-01-01

    We show that a large class of phenomenologically viable models for gauge mediation of supersymmetry breaking based on meta-stable vacua can be realized in local Calabi–Yau compactifications of string theory.

  9. 14 CFR 1252.402 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Mediation. 1252.402 Section 1252.402... Procedures § 1252.402 Mediation. (a) Referral of complaints for mediation. NASA will refer to the Federal Mediation and Conciliation Service all complaints that: (1) Fall within the jurisdiction of the Act...

  10. Mediation in Schools: Tapping the Potential

    Science.gov (United States)

    Hendry, Richard

    2010-01-01

    This article explores the developing role of mediation as a conflict resolution process in schools. It gives an accepted definition and clarifies the purposes of mediation, outlining the range of contexts in and beyond schools in which mediation is already offered as a formal intervention. The typical process of mediation itself is described. The…

  11. 45 CFR 91.43 - Mediation.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Mediation. 91.43 Section 91.43 Public Welfare... Enforcement Procedures § 91.43 Mediation. (a) HHS will promptly refer to a mediation agency designated by the... mediation process to the extent necessary to reach an agreement or make an informed judgment that...

  12. 38 CFR 18.543 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Mediation. 18.543 Section... Enforcement Procedures § 18.543 Mediation. (a) Referral of complaints for mediation. VA will refer to the Federal Mediation and Conciliation Service all complaints that: (1) Fall within the jurisdiction of...

  13. Mediation and Counseling Services: A Viable Partnership

    Science.gov (United States)

    Hodges, Shannon

    2009-01-01

    Mediation has become common in many areas of society, including marital dissolution, community disputes, governmental agencies, and business and industry. Though higher education has been slower than society to adopt mediation services, campus mediation is becoming increasingly more common. This article explains why mediation is a viable…

  14. 45 CFR 1156.16 - Mediation.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Mediation. 1156.16 Section 1156.16 Public Welfare... Procedures § 1156.16 Mediation. (a) Referral of complaints for mediation. The Endowment will promptly refer all complaints to the agency designated by the Secretary of HHS to manage the mediation process...

  15. 15 CFR 923.54 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Mediation. 923.54 Section 923.54... Mediation. (a) Section 307(h) of the Act provides for mediation of serious disagreement between any Federal... cases, mediation by the Secretary, with the assistance of the Executive Office of the President, may...

  16. 15 CFR 20.12 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Mediation. 20.12 Section 20.12... Procedures § 20.12 Mediation. (a) DOC will refer to a mediation service designated by the Secretary all... further processing. (b) Both the complainant and the recipient shall participate in the mediation...

  17. 24 CFR 146.35 - Mediation.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Mediation. 146.35 Section 146.35... ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.35 Mediation. (a) HUD shall refer to the Federal Mediation and Conciliation Service, a mediation agency designated by the Secretary...

  18. 10 CFR 1040.89-6 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Mediation. 1040.89-6 Section 1040.89-6 Energy DEPARTMENT... Enforcement Procedures § 1040.89-6 Mediation. (a) Referral of complaints for mediation. DOE will refer to the Federal Mediation and Conciliation Service, in accordance with 45 CFR 90.43(c)(3), all complaints that:...

  19. 43 CFR 17.332 - Mediation.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Mediation. 17.332 Section 17.332 Public..., and Enforcement Procedures § 17.332 Mediation. (a) Referral of complaints for mediation. DOI will... participate in the mediation process to the extent necessary to reach an agreement or make an...

  20. 7 CFR 614.11 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Mediation. 614.11 Section 614.11 Agriculture... AGRICULTURE CONSERVATION OPERATIONS NRCS APPEAL PROCEDURES § 614.11 Mediation. (a) A participant who wishes to pursue mediation must file request for mediation under this part with the NRCS official designated in...

  1. 22 CFR 218.33 - Mediation.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Mediation. 218.33 Section 218.33 Foreign... § 218.33 Mediation. (a) Referral of complaints for mediation. The agency will refer to the Federal Mediation and Conciliation Service all complaints that: (1) fall within the jurisdiction of...

  2. 15 CFR 930.111 - OCRM mediation.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false OCRM mediation. 930.111 Section 930... FEDERAL CONSISTENCY WITH APPROVED COASTAL MANAGEMENT PROGRAMS Secretarial Mediation § 930.111 OCRM mediation. The availability of mediation does not preclude use by the parties of alternative means...

  3. 10 CFR 4.333 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Mediation. 4.333 Section 4.333 Energy NUCLEAR REGULATORY... Investigation, Conciliation, and Enforcement Procedures § 4.333 Mediation. (a) Referral of complaints for mediation. NRC will refer to a mediation agency designated by the Secretary of the Department of Health...

  4. 7 CFR 780.9 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Mediation. 780.9 Section 780.9 Agriculture Regulations... PROGRAMS APPEAL REGULATIONS § 780.9 Mediation. (a) Any request for mediation must be submitted after... once: (1) If resolution of an adverse decision is not achieved in mediation, a participant may...

  5. Mediation in Legal English Teaching

    Directory of Open Access Journals (Sweden)

    Chovancová Barbora

    2016-06-01

    Full Text Available Mediation is a language activity that has been unjustly neglected when preparing law students for their future professional careers. When trained in a professional context, students need to develop and improve complex communicative skills. These include not only the traditional language skills such as reading, writing, listening and speaking, but also more advanced skills such as summarizing, providing definitions, changing registers etc. All these are involved in the students’ acquisition of ‘soft skills’ that are particularly important for students of law since much of their future work involves interpersonal lawyer-client interaction. This article argues that mediation is a crucial (though previously underestimated skill and that law-oriented ESP instruction should provide training aimed at developing this skill. Showing a practical application of this approach, the paper demonstrates that mediation can be successfully integrated in the legal English syllabus and make the learning of legal English more effective.

  6. Assistive Technology and Affective Mediation

    Directory of Open Access Journals (Sweden)

    Nestor Garay

    2006-01-01

    Full Text Available The lack of attention towards affective communication in assistive technology and disability research can be potentially overcome thanks to the development of affective computing and affective mediation areas. This document describes the main impairments and disorders that may involve affective communication deficits. We also present several affective mediation technologies that are being applied or may be integrated in assistive technologies in order to improve affective communication for a range of disabilities. Finally, we describe our experience with Gestele, an application that incorporates multimodal elements of affective mediation for people with mobility impairments, as well as the results of an empirical study conducted to evaluate the application’s validity in communication via telephone.

  7. Three tasks for mediatization research

    DEFF Research Database (Denmark)

    Ekstrøm, Mats; Fornäs, Johan; Jansson, André;

    2016-01-01

    Based on the interdisciplinary experience of a Swedish research committee, this article discusses critical conceptual issues raised by the current debate on mediatization – a concept that holds great potential to constitute a space for synthesized understandings of media-related social...... that mediatization researchers have sometimes formulated too grand claims as to mediatization’s status as a unitary approach, a meta-theory or a paradigm. Such claims have led to problematic confusions around the concept and should be abandoned in favour of a more open agenda. In line with such a call for openness...... transformations. In contrast to other, more metaphorical constructions, mediatization can be studied empirically in systematic ways through various sub-processes that together provide a complex picture of how culture and everyday life evolve in times of media saturation. The first part of this article argues...

  8. Nursing as textually mediated reality.

    Science.gov (United States)

    Cheek, J; Rudge, T

    1994-11-01

    Nursing and nursing practice both construct and are in turn constructed by the context in which they operate. Texts plays a central part in that construction. As such, nursing and nursing practice can be considered to represent a reality that is textually mediated. This paper explores the notion of nursing as a textually mediated reality and offers the reader the possibility of engaging in reflection on what implications this has for nursing and their own nursing practice. The analyses provided draw on aspects of the work of both Foucault and Derrida. Foucault's notion of discourse provides a vehicle for the exploration of nursing as textually mediated, as does Derrida's concept of binary oppositions. The paper thus illustrates some of the possibilities afforded nursing by poststructural analyses. In particular it does this by exploring one of the central textual constructions, impacting on the way that nursing and nursing practice are conceptualized, the mind/body binary opposition.

  9. Dynamical Messengers for Gauge Mediation

    Energy Technology Data Exchange (ETDEWEB)

    Hook, Anson; Torroba, Gonzalo; /SLAC /Stanford U., Phys. Dept.

    2011-08-17

    We construct models of indirect gauge mediation where the dynamics responsible for breaking supersymmetry simultaneously generates a weakly coupled subsector of messengers. This provides a microscopic realization of messenger gauge mediation where the messenger and hidden sector fields are unified into a single sector. The UV theory is SQCD with massless and massive quarks plus singlets, and at low energies it flows to a weakly coupled quiver gauge theory. One node provides the primary source of supersymmetry breaking, which is then transmitted to the node giving rise to the messenger fields. These models break R-symmetry spontaneously, produce realistic gaugino and sfermion masses, and give a heavy gravitino.

  10. Secure mediated certificateless signature scheme

    Institute of Scientific and Technical Information of China (English)

    YANG Chen; MA Wen-ping; WANG Xin-mei

    2007-01-01

    Ju et al proposed a certificateless signature scheme with instantaneous revocation by introducing security mediator (SEM) mechanism. This article presents a detailed cryptoanalysis of this scheme and shows that, in their proposed scheme, once a valid signature has been produced, the signer can recover his private key information and the instantaneous revocation property will be damaged. Furthermore, an improved mediated signature scheme, which can eliminate these disadvantages, is proposed, and security proof of the improved scheme under elliptic curve factorization problem (ECFP) assumption and bilinear computational diffie-hellman problem (BCDH) assumption is also proposed.

  11. The herpes simplex virus 1-encoded envelope glycoprotein B activates NF-κB through the Toll-like receptor 2 and MyD88/TRAF6-dependent signaling pathway.

    Directory of Open Access Journals (Sweden)

    Mingsheng Cai

    Full Text Available The innate immune response plays a critical role in the host defense against invading pathogens, and TLR2, a member of the Toll-like receptor (TLR family, has been implicated in the immune response and initiation of inflammatory cytokine secretion against several human viruses. Previous studies have demonstrated that infectious and ultraviolet-inactivated herpes simplex virus 1 (HSV-1 virions lead to the activation of nuclear factor kappa B (NF-κB and secretion of proinflammatory cytokines via TLR2. However, except for the envelope glycoprotein gH and gL, whether there are other determinants of HSV-1 responsible for TLR2 mediated biological effects is not known yet. Here, we demonstrated that the HSV-1-encoded envelope glycoprotein gB displays as molecular target recognized by TLR2. gB coimmunoprecipitated with TLR2, TLR1 and TLR6 in transfected and infected human embryonic kidney (HEK 293T cells. Treatment of TLR2-transfected HEK293T (HEK293T-TLR2 cells with purified gB results in the activation of NF-κB reporter, and this activation requires the recruitment of the adaptor molecules myeloid differentiation primary-response protein 88 (MyD88 and tumor necrosis factor receptor-associated factor 6 (TRAF6 but not CD14. Furthermore, activation of NF-κB was abrogated by anti-gB and anti-TLR2 blocking antibodies. In addition, the expression of interleukin-8 induced by gB was abrogated by the treatment of the human monocytic cell line THP-1 with anti-TLR2 blocking antibody or by the incubation of gB with anti-gB antibody. Taken together, these results indicate the importance and potency of HSV-1 gB as one of pathogen-associated molecular patterns (PAMPs molecule recognized by TLR2 with immediate kinetics.

  12. Should Lawyers Act as Mediators?

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    On September 1, the Henan Provincial Department of Justice and the Henan Higher People’s Court jointly issued a document. It suggests lawyers do as much as possible to mediate between litigants involved in lawsuits of civil disputes and misdemeanor lawsuits, in order to help them

  13. Three tasks for mediatization research

    DEFF Research Database (Denmark)

    Ekstrøm, Mats; Fornäs, Johan; Jansson, André

    2016-01-01

    Based on the interdisciplinary experience of a Swedish research committee, this article discusses critical conceptual issues raised by the current debate on mediatization – a concept that holds great potential to constitute a space for synthesized understandings of media-related social transforma......Based on the interdisciplinary experience of a Swedish research committee, this article discusses critical conceptual issues raised by the current debate on mediatization – a concept that holds great potential to constitute a space for synthesized understandings of media-related social...... transformations. In contrast to other, more metaphorical constructions, mediatization can be studied empirically in systematic ways through various sub-processes that together provide a complex picture of how culture and everyday life evolve in times of media saturation. The first part of this article argues...... that mediatization researchers have sometimes formulated too grand claims as to mediatization’s status as a unitary approach, a meta-theory or a paradigm. Such claims have led to problematic confusions around the concept and should be abandoned in favour of a more open agenda. In line with such a call for openness...

  14. Mediated Encryption: Analysis and Design

    Directory of Open Access Journals (Sweden)

    I. Elashry1

    2015-01-01

    Full Text Available Boneh, Ding and Tsudik presented identity-based mediated RSA encryption and signature systems in which the users are not allowed to decrypt/sign messages without the authorisation of a security mediator.We show that ID-MRSA is not secure and we present a secure modified version of it which is as efficient as the original system. We also propose a generic mediated encryption that translates any identity based encryption to a mediated version of this IBE. It envelops an IBE encrypted message using a user’s identity into an IBE envelope using the identity of the SEM. We present two security models based on the role of the adversary whether it is a revoked user or a hacked SEM. We prove that GME is as secure as the SEM’s IBE against a revoked user and as secure as the user’s IBE against a hacked SEM. We also present two implementations of GME based on Boneh-Franklin FullIBE system which is a pairing-based system and Boneh, Gentry and Hamburg (BGH system which is pairing-free system.

  15. Eicosanoids mediate insect hemocyte migration

    Science.gov (United States)

    Hemocyte chemotaxis toward infection and wound sites is an essential component of insect defense reactions, although the biochemical signal mechanisms responsible for mediating chemotaxis in insect cells are not well understood. Here we report on the outcomes of experiments designed to test the hyp...

  16. Anomaly mediation in superstring theory

    Energy Technology Data Exchange (ETDEWEB)

    Conlon, J.P. [Rudolf Peierls Center for Theoretical Physics, 1 Keble Road, Oxford, OX1 3NP (United Kingdom); Balliol College, Oxford, OX1 3BJ (United Kingdom); Kavli Institute of Theoretical Physics, Kohn Hall, University of California, Santa Barbara CA 93106-4030 (United States); Goodsell, M. [Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22603 Hamburg (Germany); Palti, E. [Centre de Physique Theorique, Ecole Polytechnique, CNRS, 91128 Palaiseau (France)

    2011-01-15

    We study anomaly mediated supersymmetry breaking in type IIB string theory and use our results to test the supergravity formula for anomaly mediated gaugino masses. We compute 1-loop gaugino masses for models of D3-branes on orbifold singularities with 3-form fluxes by calculating the annulus correlator of 3-form flux and two gauginos in the zero momentum limit. Consistent with supergravity expectations we find both anomalous and running contributions to 1-loop gaugino masses. For background Neveu-Schwarz H-flux we find an exact match with the supergravity formula. For Ramond-Ramond flux there is an off-shell ambiguity that precludes a full matching. The anomaly mediated gaugino masses, while determined by the infrared spectrum, arise from an explicit sum over UV open string winding modes. We also calculate brane-to-brane tree-level gravity mediated gaugino masses and show that there are two contributions coming from the dilaton and from the twisted modes, which are suppressed by the full T{sup 6} volume and the untwisted T{sup 2} volume respectively. (Copyright copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  17. Anomaly mediation in superstring theory

    Energy Technology Data Exchange (ETDEWEB)

    Conlon, Joseph P. [Rudolf Peierls Center for Theoretical Physics, Oxford (United Kingdom); Balliol College, Oxford (United Kingdom); Goodsell, Mark [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Palti, Eran [Centre de Physique Theoretique, Ecole Polytechnique, CNRS, Palaiseau (France)

    2010-08-15

    We study anomaly mediated supersymmetry breaking in type IIB string theory and use our results to test the supergravity formula for anomaly mediated gaugino masses. We compute 1-loop gaugino masses for models of D3-branes on orbifold singularities with 3-form fluxes by calculating the annulus correlator of 3-form flux and two gauginos in the zero momentum limit. Consistent with supergravity expectations we find both anomalous and running contributions to 1-loop gaugino masses. For background Neveu-Schwarz H-flux we find an exact match with the supergravity formula. For Ramond-Ramond flux there is an off-shell ambiguity that precludes a full matching. The anomaly mediated gaugino masses, while determined by the infrared spectrum, arise from an explicit sum over UV open string winding modes. We also calculate brane-to-brane tree-level gravity mediated gaugino masses and show that there are two contributions coming from the dilaton and from the twisted modes, which are suppressed by the full T{sup 6} volume and the untwisted T{sup 2} volume respectively. (orig.)

  18. Parameter space of general gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Rajaraman, Arvind [Department of Physics and Astronomy, University of California, Irvine, CA 92697 (United States)], E-mail: arajaram@uci.edu; Shirman, Yuri [Department of Physics and Astronomy, University of California, Irvine, CA 92697 (United States)], E-mail: yshirman@uci.edu; Smidt, Joseph [Department of Physics and Astronomy, University of California, Irvine, CA 92697 (United States)], E-mail: jsmidt@uci.edu; Yu, Felix [Department of Physics and Astronomy, University of California, Irvine, CA 92697 (United States)], E-mail: felixy@uci.edu

    2009-07-27

    We study a subspace of General Gauge Mediation (GGM) models which generalize models of gauge mediation. We find superpartner spectra that are markedly different from those of typical gauge and gaugino mediation scenarios. While typical gauge mediation predictions of either a neutralino or stau next-to-lightest supersymmetric particle (NLSP) are easily reproducible with the GGM parameters, chargino and sneutrino NLSPs are generic for many reasonable choices of GGM parameters.

  19. Notion of Mediators in Human Interaction

    OpenAIRE

    Josip Stepanic

    2003-01-01

    Many types of human interaction are mediated processes. However, regarding the details of human description, the mediating unit structure and dynamics is not developed appropriately. The explicit concentration on mediators contributes to understanding of interplay between value sets governing interaction, interaction roles in regular activities and interaction design for purpose. This article is a contribution to development of a concept of mediated human interaction through structuring of me...

  20. Introduction: Mediating and Remediating Death

    DEFF Research Database (Denmark)

    Christensen, Dorthe Refslund; Sandvik, Kjetil

    2014-01-01

    In this second volume we explore how people, groups and institutions deal with death through processes of mediation (the presentation of something through media), remediation (the representation of one medium in another, see below) and mediatization (the process through which core elements...... of a social or cultural activity assume media form, see below). The volume presents a wide variety of ethnographies of death from Norway, Finland, Sweden, the US, Papua New Guinea, Bosnia and Hercegovina, Libya, Tibet, Uganda and Denmark as well as a number of online sites and social media material....... These are analyzed through a vast number of theoretical and analytical perspectives in order to investigate how very diverse practices surrounding death and dying - mourning and commemoration, ritualization, politicization, re-enactment, traditionalization, activism or documentarism: private or public, offline...

  1. Vernalization-mediated chromatin changes.

    Science.gov (United States)

    Zografos, Brett R; Sung, Sibum

    2012-07-01

    Proper flowering time is vital for reproductive fitness in flowering plants. In Arabidopsis, vernalization is mediated primarily through the repression of a MADS box transcription factor, FLOWERING LOCUS C (FLC). The induction of a plant homeodomain-containing protein, VERNALIZATION INSENSITIVE 3 (VIN3), by vernalizing cold is required for proper repression of FLC. One of a myriad of changes that occurs after VIN3 is induced is the establishment of FLC chromatin at a mitotically repressed state due to the enrichment of repressive histone modifications. VIN3 induction by cold is the earliest known event during the vernalization response and includes changes in histone modifications at its chromatin. Here, the current understanding of the vernalization-mediated chromatin changes in Arabidopsis is discussed, with a focus on the roles of shared chromatin-modifying machineries in regulating VIN3 and FLC gene family expression during the course of vernalization.

  2. On Anomaly Mediated SUSY Breaking

    CERN Document Server

    de Alwis, S P

    2008-01-01

    A discrepancy between the Anomaly Mediated Supersymmetry Breaking (AMSB) gaugino mass calculated from the work of Kaplunovsky and Louis (hep-th/9402005) (KL) and other calculations in the literature is explained, and it is argued that the KL expression is the correct one relevant to the Wilsonian action. Furthermore it is argued that the AMSB contribution to the squark and slepton masses should be replaced by the contribution pointed out by Dine and Seiberg (DS) which has nothing to do with Weyl anomalies. This is not in general equivalent to the AMSB expression, and it is shown that there are models in which the usual AMSB expression would vanish but the DS one is non-zero. In fact the latter has aspects of both AMSB and gauge mediated SUSY breaking. In particular like the latter, it gives positive squared masses for sleptons.

  3. Mediatization: a concept, multiple voices

    Directory of Open Access Journals (Sweden)

    Pedro Gilberto GOMES

    2016-12-01

    Full Text Available Mediatization has become increasingly a key concept, fundamental, essential to describe the present and the history of media and communicative change taking place. Thus, it became part of a whole, one can not see them as a separate sphere. In this perspective, the media coverage is used as a concept to describe the process of expansion of the different technical means and consider the interrelationships between the communicative change, means and sociocultural change. However, although many researchers use the concept of mediatization, each gives you the meaning that best suits your needs. Thus, the concept of media coverage is treated with multiple voices. This paper discusses this problem and present a preliminary pre-position on the matter.

  4. Cell-mediated immune response

    DEFF Research Database (Denmark)

    Meyer, Sonja Izquierdo; Fuglsang, Katrine; Blaakaer, Jan

    2014-01-01

    OBJECTIVE: This clinical review aims to assess the efficacy of human papillomavirus 16/18 (HPV16/18) vaccination on the cell-mediated immune response in women with existing cervical intraepithelial neoplasia or cervical cancer induced by HPV16 or HPV18. DATA SOURCES AND STUDY SELECTION: A focused...... and thorough literature search conducted in five different databases found 996 publications. Six relevant articles were chosen for further review. In total, 154 patients (>18 years of age) were enrolled in prospective study trials with 3-15 months of follow up. The vaccine applications were administered two...... triggered a detectable cell-mediated immune response, some of which were statistically significant. Correlations between immunological response and clinical outcome (histopathology) were not significant, so neoplasms may not be susceptible to vaccine-generated cytotoxic T cells (CD8(+)). CONCLUSIONS...

  5. General Gauge Mediation and Deconstruction

    CERN Document Server

    McGarrie, Moritz

    2010-01-01

    We locate a supersymmetry breaking hidden sector and supersymmetric standard model on different lattice points of an orbifold moose. The hidden sector is encoded in a set of current correlators and the effects of the current correlators are mediated by the lattice site gauge groups with "lattice hopping" functions and through the bifundamental matter that links the lattice sites together. We show how the gaugino mass, scalar mass and Casimir energy of the lattice can be computed for a general set of current correlators and then give specific formulas when the hidden sector is specified to be a generalised messenger sector coupled to a supersymmetry breaking spurion. The results reproduce the effect of five dimensional gauge mediation from a purely four dimensional construction.

  6. Mediational Competencies for Online Education

    Directory of Open Access Journals (Sweden)

    María Elena Chan Núñez

    2005-11-01

    Full Text Available Addressed in the article is a position taken within and in favor of education and virtuality, considering the importance of training constructors of the digital environment. The competencies needed by actors of educational processes, the same which are necessary for their construction, are conceptualized as mediational. Because these are not usually the competencies most visibly when teachers and students are trained for online education, we found it of interest to present part of a research project on this type of competencies. The work starts out from an axiological position on virtual education, the recognition of the way the technologies model educational interactions on line. It follows with the notion of mediation and meditational competency, and comes to a design model that would consider these competencies in the development of learning environments. The article closes with reflections about the interdisciplinary integration necessary for a technological and educational development based on a communicative paradigm.

  7. Methods for Mediation Analysis with Missing Data

    Science.gov (United States)

    Zhang, Zhiyong; Wang, Lijuan

    2013-01-01

    Despite wide applications of both mediation models and missing data techniques, formal discussion of mediation analysis with missing data is still rare. We introduce and compare four approaches to dealing with missing data in mediation analysis including list wise deletion, pairwise deletion, multiple imputation (MI), and a two-stage maximum…

  8. Mediatized Extreme Right Activism and Discourse

    DEFF Research Database (Denmark)

    Peters, Rikke Alberg

    2015-01-01

    and mediatized activism. In order to analyse of the protest form, the visual aesthetics and the discourse of ‘The Immortals’, the paper mobilises two concepts from media and communication studies: mediation and mediatization. It will be argued that that the current transformation of the extreme right: that is...

  9. 7 CFR 900.109 - Mediation agreement.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Mediation agreement. 900.109 Section 900.109 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... Mediation agreement. An agreement arrived at by mediation shall not become effective until approved by...

  10. 34 CFR 300.506 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Mediation. 300.506 Section 300.506 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.506 Mediation. (a... due process complaint, to resolve disputes through a mediation process. (b) Requirements....

  11. 45 CFR 617.10 - Mediation.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Mediation. 617.10 Section 617.10 Public Welfare... OF AGE IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE FROM NSF § 617.10 Mediation. (a) NSF will refer to the Federal Mediation and Conciliation Service all complaints that fall...

  12. Causal Mediation Analysis: Warning! Assumptions Ahead

    Science.gov (United States)

    Keele, Luke

    2015-01-01

    In policy evaluations, interest may focus on why a particular treatment works. One tool for understanding why treatments work is causal mediation analysis. In this essay, I focus on the assumptions needed to estimate mediation effects. I show that there is no "gold standard" method for the identification of causal mediation effects. In…

  13. 34 CFR 303.419 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Mediation. 303.419 Section 303.419 Education... DISABILITIES Procedural Safeguards Mediation and Due Process Procedures for Parents and Children § 303.419 Mediation. (a) General. Each State shall ensure that procedures are established and implemented to...

  14. 7 CFR 205.663 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Mediation. 205.663 Section 205.663 Agriculture... PROVISIONS NATIONAL ORGANIC PROGRAM Administrative Compliance § 205.663 Mediation. Any dispute with respect... acceptance by the certifying agent. Mediation shall be requested in writing to the applicable...

  15. 41 CFR 101-8.717 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Mediation. 101-8.717... FINANCIAL ASSISTANCE 8.7-Discrimination Prohibited on the Basis of Age § 101-8.717 Mediation. (a) GSA promptly refers to the mediation agency designated by the Secretary, HHS, all sufficient complaints...

  16. 7 CFR 400.94 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Mediation. 400.94 Section 400.94 Agriculture... AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Appeal Procedure § 400.94 Mediation. For adverse decisions only: (a) Appellants have the right to seek mediation or other forms of alternative dispute resolution...

  17. 13 CFR 117.12 - Mediation.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Mediation. 117.12 Section 117.12... Mediation. (a) SBA shall, after ensuring that the complaint falls within the coverage of this Act and all... clearly within an exception, promptly refer the complaint to the Federal Mediation and...

  18. Single-Level and Multilevel Mediation Analysis

    Science.gov (United States)

    Tofighi, Davood; Thoemmes, Felix

    2014-01-01

    Mediation analysis is a statistical approach used to examine how the effect of an independent variable on an outcome is transmitted through an intervening variable (mediator). In this article, we provide a gentle introduction to single-level and multilevel mediation analyses. Using single-level data, we demonstrate an application of structural…

  19. Agents Which Mediate Pulmonary Edema

    Science.gov (United States)

    1990-12-01

    directly the effect of mediators in the lavage on pulmonary vascular endothelium using endothelial cell line methods, as well as isolated perfused lungs...inte;wvention and/or protection from pathological chang6s. 8 1 MATERIALS AND METHODS Bronchoalveolar Lavage: The lavage procedure was performed through...have changed our 14 I technique to the following: 1 ) animals are lightly anesthetized with 30 mg/kg sodium arnytal and a #7.5 - 8 F polyvinyl

  20. Mediating states in 180Ta

    OpenAIRE

    Alexa, Petr

    2002-01-01

    Possible theoretical electromagnetic paths between the ground state and the isomeric state at 75.3 keV in 180Ta are discussed in the framework of the two-quasiparticle-plus-phonon model and the standard axially-symmetric rotor model including Coriolis mixing. Experimental transition rates from the isomeric state to the ground state via observed mediating states are compared to the theoretical ones.

  1. SIRT1-mediated acute cardioprotection

    OpenAIRE

    Nadtochiy, Sergiy M; Yao, Hongwei; McBurney, Michael W.; Gu, Wei; Guarente, Leonard; Rahman, Irfan; Paul S. Brookes

    2011-01-01

    Overexpression studies have revealed a role for silent information regulator of transcription 1 (SIRT1) lysine deacetylase in cardioprotection against ischemia-reperfusion injury via long-term transcriptional effects. However, short-term SIRT1-mediated lysine deacetylation, within the context of acute cardioprotection, is poorly understood. In this study, the role of SIRT1 in the acute cardioprotective paradigm of first window ischemic preconditioning (IPC) was studied using SIRT1-deficient (...

  2. CELLULAR INTERACTIONS MEDIATED BY GLYCONECTIDS

    OpenAIRE

    Popescu, O.; Sumanovski, L. T.; I. Checiu; Elisabeta Popescu; G. N. Misevic

    1999-01-01

    Cellular interactions involve many types of cell surface molecules and operate via homophilic and/or heterophilic protein-protein and protein-carbohydrate binding. Our investigations in different model-systems (marine invertebrates and mammals) have provided direct evidence that a novel class of primordial proteoglycans, named by us gliconectins, can mediate cell adhesion via a new alternative molecular mechanism of polyvalent carbohydrate-carbohydrate binding. Biochemical characterization of...

  3. Substrate mediated enzyme prodrug therapy.

    Directory of Open Access Journals (Sweden)

    Betina Fejerskov

    Full Text Available In this report, we detail Substrate Mediated Enzyme Prodrug Therapy (SMEPT as a novel approach in drug delivery which relies on enzyme-functionalized cell culture substrates to achieve a localized conversion of benign prodrug(s into active therapeutics with subsequent delivery to adhering cells or adjacent tissues. For proof-of-concept SMEPT, we use surface adhered micro-structured physical hydrogels based on poly(vinyl alcohol, β-glucuronidase enzyme and glucuronide prodrugs. We demonstrate enzymatic activity mediated by the assembled hydrogel samples and illustrate arms of control over rate of release of model fluorescent cargo. SMEPT was not impaired by adhering cells and afforded facile time - and dose - dependent uptake of the in situ generated fluorescent cargo by hepatic cells, HepG2. With the use of a glucuronide derivative of an anticancer drug, SN-38, SMEPT afforded a decrease in cell viability to a level similar to that achieved using parent drug. Finally, dose response was achieved using SMEPT and administration of judiciously chosen concentration of SN-38 glucuronide prodrug thus revealing external control over drug delivery using drug eluting surface. We believe that this highly adaptable concept will find use in diverse biomedical applications, specifically surface mediated drug delivery and tissue engineering.

  4. Mediation With Muscle: Understanding When Mediators Commit Resources to Civil War Negotiations

    Science.gov (United States)

    2015-12-01

    NAVAL POSTGRADUATE SCHOOL MONTEREY, CALIFORNIA THESIS Approved for public release; distribution is unlimited MEDIATION WITH MUSCLE...UNDERSTANDING WHEN MEDIATORS COMMIT RESOURCES TO CIVIL WAR NEGOTIATIONS by Michael D. Caplan December 2015 Thesis Advisor: T. Camber Warren Second Reader...TYPE AND DATES COVERED Master’s thesis 4. TITLE AND SUBTITLE MEDIATION WITH MUSCLE: UNDERSTANDING WHEN MEDIATORS COMMIT RESOURCES TO CIVIL WAR

  5. Construction mediation as a developmental process

    Directory of Open Access Journals (Sweden)

    Julian Sidoli del Ceno

    2013-02-01

    Full Text Available This paper seeks to argue that mediation has been hitherto conceived in the construction industry, and indeed by practitioners in other related disciplines such as property management, as largely a ‘problem-solving’ mechanism. Whilst this is clearly an aim of mediation there is also the appended danger that the value of mediation is conceived in these terms alone. If this is the case, then its value, or success, is conceived very narrowly. The aim of this paper is, then, to argue that there are wider values to mediation in a construction setting. These values can be considered as a ‘family’ of related attitudes, skills and perceptions that can positively affect the persons involved. By affecting growth in individuals an organisational change may follow. This, in turn, can result in a significant cultural change in the industry, and associated professions as a whole, as well as having a positive impact on construction education. The paper begins by an overview of the development of mediation and proceeds to consider its current use of mediation in construction. It then considers the question of how mediation success is conceived. The paper argues that both the current practice of construction mediation and the way in which its success is measured are too narrow. It argues that a wider approach to construction mediation is required. Finally, drawing from the literature on ‘idealist’ mediation an account of mediation as a developmental process is developed.

  6. Mediation et violences conjugales Mediation and Domestic Violence

    Directory of Open Access Journals (Sweden)

    Jacques Faget

    2009-11-01

    Full Text Available Ce travail analyse  les potentialités et les limites du recours à la médiation en matière de régulation des violences conjugales.S’appuyant sur une étude empirique de sa mise en œuvre en France et sur la littérature scientifique existante il révèle l’existence de deux modèles de pratique reflétant deux conceptions de la médiation et plus généralement de l’articulation entre régulations pénales et sociales.This paper analyzes the potential and limits of recourse to mediation for regulating domestic violence. On the basis of an empirical study of its implementation in France and of the existing academic literature, it shows the existence of two types of practices reflecting two conceptions of mediation and more generally, two conceptions of the articulation between social and penal regulation. Full Text

  7. Universality in pure gravity mediation

    Energy Technology Data Exchange (ETDEWEB)

    Evans, Jason L.; Olive, Keith A. [University of Minnesota, William I. Fine Theoretical Physics Institute, School of Physics and Astronomy, Minneapolis, MN (United States); Ibe, Masahiro [University of Tokyo, ICRR, Kashiwa (Japan); University of Tokyo, Kavli IPMU, TODIAS, Kashiwa (Japan); Yanagida, Tsutomu T. [University of Tokyo, Kavli IPMU, TODIAS, Kashiwa (Japan)

    2013-07-15

    If low-energy supersymmetry is realized in nature, the apparent discovery of a Higgs boson with mass around 125 GeV suggests a supersymmetric mass spectrum in the TeV or multi-TeV range. Multi-TeV scalar masses are a necessary component of supersymmetric models with pure gravity mediation or in any model with strong moduli stabilization. Here, we show that full scalar mass universality remains viable as long as the ratio of Higgs vevs, tan{beta}, is relatively small (

  8. Transporter-mediated biofuel secretion.

    Science.gov (United States)

    Doshi, Rupak; Nguyen, Tuan; Chang, Geoffrey

    2013-05-07

    Engineering microorganisms to produce biofuels is currently among the most promising strategies in renewable energy. However, harvesting these organisms for extracting biofuels is energy- and cost-intensive, limiting the commercial feasibility of large-scale production. Here, we demonstrate the use of a class of transport proteins of pharmacological interest to circumvent the need to harvest biomass during biofuel production. We show that membrane-embedded transporters, better known to efflux lipids and drugs, can be used to mediate the secretion of intracellularly synthesized model isoprenoid biofuel compounds to the extracellular milieu. Transporter-mediated biofuel secretion sustainably maintained an approximate three- to fivefold boost in biofuel production in our Escherichia coli test system. Because the transporters used in this study belong to the ubiquitous ATP-binding cassette protein family, we propose their use as "plug-and-play" biofuel-secreting systems in a variety of bacteria, cyanobacteria, diatoms, yeast, and algae used for biofuel production. This investigation showcases the potential of expressing desired membrane transport proteins in cell factories to achieve the export or import of substances of economic, environmental, or therapeutic importance.

  9. Legal and Psychological Aspects of Mediation

    Directory of Open Access Journals (Sweden)

    Dobrokhotova E. N.

    2016-01-01

    Full Text Available The article focuses on gradual innovation of mediation into the practice of social conflict resolution in the light of legal and psychological means of mediation. While mediation is perceived as a conflictological concept and is more widely used in dispute settlement and resolution, a new interdisciplinary field of theoretical knowledge with its own conceptual framework as well as a new professional and practical field are beginning to form both in Russia and in other countries. As theoretical and practical aspects of innovation in mediation require consolidation not only for its national development but also for the guaranteed international cooperation, the article touches upon some of the particular theoretical issues of the topic in question: terminological consistency, consolidation of the system of mediation principles, the phenomenon of juridisation of mediation and its limits.

  10. Integrating Mediators and Moderators in Research Design

    OpenAIRE

    MacKinnon, David P.

    2011-01-01

    The purpose of this article is to describe mediating variables and moderating variables and provide reasons for integrating them in outcome studies. Separate sections describe examples of moderating and mediating variables and the simplest statistical model for investigating each variable. The strengths and limitations of incorporating mediating and moderating variables in a research study are discussed as well as approaches to routinely including these variables in outcome research. The rout...

  11. Focus point supersymmetry in extended gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Ran [School of Physics, Nankai University,Tianjin 300071 (China); Li, Tianjun [State Key Laboratory of Theoretical Physics and Kavli Institute for Theoretical Physics (KITPC),Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 100190 (China); School of Physical Electronics, University of Electronic Science and Technology of China,Chengdu 610054 (China); Staub, Florian [Bethe Center for Theoretical Physics & Physikalisches Institut der Universität Bonn,Nußallee 12, 53115 Bonn (Germany); Zhu, Bin [School of Physics, Nankai University,Tianjin 300071 (China)

    2014-03-27

    We propose a small extension of the minimal gauge mediation through the combination of extended gauge mediation and conformal sequestering. We show that the focus point supersymmetry can be realized naturally, and the fine tuning is significantly reduced compared to the minimal gauge mediation and extended gauge mediation without focus point. The Higgs boson mass is around 125 GeV, the gauginos remain light, and the gluino is likely to be detected at the next run of the LHC. However, the multi-TeV squarks is out of the reach of the LHC. The numerical calculation for fine-tuning shows that this model remains natural.

  12. Parental mediation and cyberbullying - a longitudinal study.

    Science.gov (United States)

    Chng, Grace S; Liau, Albert; Khoo, Angeline; Li, Dongdong

    2014-01-01

    Parents use active and restrictive mediation strategies to guide and regulate children's online participation and the online risks they encounter. However, changes in parental mediation do occur over time and the effectiveness of these strategies on cyberbullying demands for further empirical investigation. The current study addresses these issues with a sample of 1084 students (49% girls) in a longitudinal, three-wave design. Gender differences were tested via multi-group analyses. Longitudinal growth models showed that parental use of both active and restrictive mediation decreased over time. For both types of mediation, the mean rate of change had a significant effect on boys' engagement in cyberbullying, but not for girls. Initial levels of restrictive mediation, but not active mediation, were found to be significantly predictive of cyberbullying in both genders. Girls had higher initial levels of both parental mediation types in comparison to boys. The results reveal that the effectiveness of active and restrictive mediation in relation to students' cyberbullying differs and informs us on gender differences. The implications of these results for parental education in online mediation are discussed.

  13. Phenomenological aspects of mirage mediation

    Energy Technology Data Exchange (ETDEWEB)

    Loewen, Valeri

    2009-07-15

    We consider the possibility that string theory vacua with spontaneously broken supersymmetry and a small positive cosmological constant arise due to hidden sector matter interactions, known as F-uplifting/F-downlifting. We analyze this procedure in a model-independent way in the context of type IIB and heterotic string theory. Our investigation shows that the uplifting/downlifting sector has very important consequences for the resulting phenomenology. Not only does it adjust the vacuum energy, but it can also participate in the process of moduli stabilization. In addition, we find that this sector is the dominant source of supersymmetry breaking. It leads to a hybrid mediation scheme and its signature is a relaxed mirage pattern of the soft supersymmetry breaking terms. The low energy spectra exhibit distinct phenomenological properties and di er from conventional schemes considered so far. (orig.)

  14. Lepton-Flavor Violating Mediators

    CERN Document Server

    Galon, Iftah; Tanedo, Philip

    2016-01-01

    We present a framework where dark matter interacts with the Standard Model through a light, spin-0 mediator that couples chirally to pairs of different-flavor leptons. This flavor violating final state weakens bounds on new physics coupled to leptons from terrestrial experiments and cosmic-ray measurements. As an example, we apply this framework to construct a model for the Fermi-LAT excess of GeV $\\gamma$-rays from the galactic center. We comment on the viability of this portal for self-interacting dark matter explanations of small scale structure anomalies and embeddings in flavor models. Models of this type are shown to be compatible with the muon anomalous magnetic moment anomaly. We review current experimental constraints and identify possible future theoretical and experimental directions.

  15. Palladium-mediated intracellular chemistry

    Science.gov (United States)

    Yusop, Rahimi M.; Unciti-Broceta, Asier; Johansson, Emma M. V.; Sánchez-Martín, Rosario M.; Bradley, Mark

    2011-03-01

    Many important intracellular biochemical reactions are modulated by transition metals, typically in the form of metalloproteins. The ability to carry out selective transformations inside a cell would allow researchers to manipulate or interrogate innumerable biological processes. Here, we show that palladium nanoparticles trapped within polystyrene microspheres can enter cells and mediate a variety of Pd0-catalysed reactions, such as allylcarbamate cleavage and Suzuki-Miyaura cross-coupling. The work provides the basis for the customization of heterogeneous unnatural catalysts as tools to carry out artificial chemistries within cells. Such in cellulo synthesis has potential for a plethora of applications ranging from cellular labelling to synthesis of modulators or inhibitors of cell function.

  16. Protooncogenes as mediators of apoptosis.

    Science.gov (United States)

    Teng, C S

    2000-01-01

    Apoptosis has been well established as a vital biological phenomenon that is important in the maintenance of cellular homeostasis. Three major protooncogene families and their encoded proteins function as mediators of apoptosis in various cell types and are the subject of this chapter. Protooncogenic proteins such as c-Myc/Max, c-Fos/c-Jun, and Bcl-2/Bax utilize a synergetic effect to enhance their roles in the pro- or antiapoptotic action. These family members activate and repress the expression of their target genes, control cell cycle progression, and execute programmed cell death. Repression or overproduction of these protooncogenic proteins induces apoptosis, which may vary as a result of either cell type specificity or the nature of the apoptotic stimuli. The proapoptotic and antiapoptotic proteins exert their effects in the membrane of cellular organelles. Here they generate cell-type-specific signals that activate the caspase family of proteases and their regulators for the execution of apoptosis.

  17. Integrins mediating bone signal transduction

    Institute of Scientific and Technical Information of China (English)

    HE Chuanglong; WANG Yuanliang; YANG Lihua; ZHANG Jun

    2004-01-01

    Integrin-mediated adhesions play critical roles in diverse cell functions. Integrins offers a platform on which mechanical stimuli, cytoskeletal organization, biochemical signals can concentrate. Mechanical stimuli transmitted by integrins influence the cytoskeleton, in turn, the cytoskeleton influences cell adhesion via integrins, then cell adhesion results in a series of signal transduction cascades. In skeleton, integrins also have a key role for bone resoption by osteoclasts and reformation by osteoblasts. In present review, the proteins involved in integrin signal transduction and integrin signal transduction pathways were discussed, mainly on the basic mechanisms of integrin signaling and the roles of integrins in bone signal transduction, which may give insight into new therapeutic agents to all kinds of skeletal diseases and new strategies for bone tissue engineering.

  18. Mediatization Theory and Digital Media

    DEFF Research Database (Denmark)

    Finnemann, Niels Ole

    2011-01-01

    of application.  In Section Two the paper discusses different conceptualizations. It is argued that even if they bring new insights, they cannot be unified into one concept, and that they also lack a consistent definition of digital media. Section three provides a definition of digital media in order to identify...... new trajectories made possible by these media, which have led into a new media matrix built around the internet and mobile devices. It will be argued that the new media matrix cannot be understood from a point of view defined by the framework of 20th century mass media because digital media open new......In the 20th century, the term "media logic" was introduced to denote the influence of independent mass media on political systems and other institutions. In recent years the idea has been reworked and labelled "mediatization" to widen the framework by including new media and new areas...

  19. Forms of Mediation: The Case of Interpreter-Mediated Interactions in Medical Systems

    Science.gov (United States)

    Baraldi, Claudio

    2009-01-01

    This paper analyses the forms of mediation in interlinguistic interactions performed in Italian healthcare services and in contexts of migration. The literature encourages dialogic transformative mediation, empowering participants' voices and changing cultural presuppositions in social systems. It may be doubtful, however, whether mediation can…

  20. Designing business rules for mediation : a process towards agent-mediated business coordination

    NARCIS (Netherlands)

    Zhao, Z.; Dignum, M.V.; Dignum, F.P.M.

    2008-01-01

    Business process integration is a very active research area, in which mediation is one of the fundamental architectural choices. Mediators have difficulties to design mediation services that meet the requirements of the different stakeholders. Business rules play an important role in the decision pr

  1. Interaction intensity and pollinator-mediated selection.

    Science.gov (United States)

    Trunschke, Judith; Sletvold, Nina; Ågren, Jon

    2017-02-27

    In animal-pollinated plants, the opportunity for selection and the strength of pollinator-mediated selection are expected to increase with the degree of pollen limitation. However, whether differences in pollen limitation can explain variation in pollinator-mediated and net selection among animal-pollinated species is poorly understood. In the present study, we quantified pollen limitation, variance in relative fitness and pollinator-mediated selection on five traits important for pollinator attraction (flowering start, plant height, flower number, flower size) and pollination efficiency (spur length) in natural populations of 12 orchid species. Pollinator-mediated selection was quantified by subtracting estimates of selection gradients for plants receiving supplemental hand-pollination from estimates obtained for open-pollinated control plants. Mean pollen limitation ranged from zero to 0.96. Opportunity for selection, pollinator-mediated selection and net selection were all positively related to pollen limitation, whereas nonpollinator-mediated selection was not. Opportunity for selection varied five-fold, strength of pollinator-mediated selection varied three-fold and net selection varied 1.5-fold among species. Supplemental hand-pollination reduced both opportunity for selection and selection on floral traits. The results show that the intensity of biotic interactions is an important determinant of the selection regime, and indicate that the potential for pollinator-mediated selection and divergence in floral traits is particularly high in species that are strongly pollen-limited.

  2. Mediating Social Conflict: A Ford Foundation Report.

    Science.gov (United States)

    Ford Foundation, New York, NY.

    Third-party intervention is increasingly recognized as an effective means of settling social, institutional, community, and interpersonal disputes. Traditionally, mediation efforts have been directed toward conflicts involving labor/management relations. Since the 1960s, however, mediation efforts have also been applied to situations involving…

  3. Semiotic Mediation within an AT Frame

    Science.gov (United States)

    Maracci, Mirko; Mariotti, Maria Alessandra

    2013-01-01

    This article is meant to present a specific elaboration of the notion of mediation in relation to the use of artefacts to enhance mathematics teaching and learning: the elaboration offered by the Theory of Semiotic Mediation. In particular, it provides an explicit model--consistent with the activity-actions-operations framework--of the actions…

  4. Integrating Mediators and Moderators in Research Design

    Science.gov (United States)

    MacKinnon, David P.

    2011-01-01

    The purpose of this article is to describe mediating variables and moderating variables and provide reasons for integrating them in outcome studies. Separate sections describe examples of moderating and mediating variables and the simplest statistical model for investigating each variable. The strengths and limitations of incorporating mediating…

  5. Parent Mediation Empowers Sibling Conflict Resolution

    Science.gov (United States)

    Ross, Hildy S.; Lazinski, Marysia J.

    2014-01-01

    Research Findings: For the current study, formal mediation procedures were adapted for families and parents were trained and asked to mediate their children's disputes; control group parents intervened as they normally would. Conflict negotiations with parents and their children (ages 3½-11 years) occurring 3 and 7 weeks following training, and…

  6. Intercultural Conflict and Mediation: An Intergroup Perspective

    Science.gov (United States)

    Rubenfeld, Sara; Clement, Richard

    2012-01-01

    This study investigates the role of components of intercultural competence in the use of intercultural mediation behaviors. Through the use of the Revised Intercultural Mediation Measure, an instrument revised by the authors, 291 Anglophone and 161 Francophone participants in Canada were asked to indicate their likelihood of employing various…

  7. Sociocultural Theory and the Mediated Learning Experience.

    Science.gov (United States)

    Kozulin, Alex

    2002-01-01

    Discusses the two theories that have contributed most to the development of the mediational approach to learning, Vygotskian sociocultural theory and Feuerstein's theory of Mediated Learning Experience. Both theories emphasize the importance of sociocultural forces in shaping a child's development and learning, and have generated a number of…

  8. Elaborative Retrieval: Do Semantic Mediators Improve Memory?

    Science.gov (United States)

    Lehman, Melissa; Karpicke, Jeffrey D.

    2016-01-01

    The elaborative retrieval account of retrieval-based learning proposes that retrieval enhances retention because the retrieval process produces the generation of semantic mediators that link cues to target information. We tested 2 assumptions that form the basis of this account: that semantic mediators are more likely to be generated during…

  9. A Conceptual Framework for Mediated Environments

    Science.gov (United States)

    Childs, Mark

    2010-01-01

    Background: Immersive virtual worlds are one of a range of different platforms that can be grouped under the concept of mediated environments, i.e. environments that create a metaphorical space in which participants can position themselves and be embodied. Synthesising the literatures concerning the various mediated environment technologies…

  10. On Mediation in Virtual Learning Environments.

    Science.gov (United States)

    Davies, Larry; Hassan, W. Shukry

    2001-01-01

    Discusses concepts of mediation and focuses on the importance of implementing comprehensive virtual learning environments. Topics include education and technology as they relate to cultural change, social institutions, the Internet and computer-mediated communication, software design and human-computer interaction, the use of MOOs, and language.…

  11. The Mediator Role in Social Work Practice.

    Science.gov (United States)

    Parsons, Ruth J.

    1991-01-01

    Notes that mediation as problem-solving intervention in social work became widely used in child custody and divorce cases, child-parent conflicts, and family disputes. Argues that mediator role is inherent in social work, and examines context for and assumptions underlying it. Discusses nature of conflicts, issue of neutrality within social work…

  12. Integrating Mediators and Moderators in Research Design.

    Science.gov (United States)

    Mackinnon, David P

    2011-11-01

    The purpose of this article is to describe mediating variables and moderating variables and provide reasons for integrating them in outcome studies. Separate sections describe examples of moderating and mediating variables and the simplest statistical model for investigating each variable. The strengths and limitations of incorporating mediating and moderating variables in a research study are discussed as well as approaches to routinely including these variables in outcome research. The routine inclusion of mediating and moderating variables holds the promise of increasing the amount of information from outcome studies by generating practical information about interventions as well as testing theory. The primary focus is on mediating and moderating variables for intervention research but many issues apply to nonintervention research as well.

  13. Meta-data based mediator generation

    Energy Technology Data Exchange (ETDEWEB)

    Critchlaw, T

    1998-06-28

    Mediators are a critical component of any data warehouse; they transform data from source formats to the warehouse representation while resolving semantic and syntactic conflicts. The close relationship between mediators and databases requires a mediator to be updated whenever an associated schema is modified. Failure to quickly perform these updates significantly reduces the reliability of the warehouse because queries do not have access to the most current data. This may result in incorrect or misleading responses, and reduce user confidence in the warehouse. Unfortunately, this maintenance may be a significant undertaking if a warehouse integrates several dynamic data sources. This paper describes a meta-data framework, and associated software, designed to automate a significant portion of the mediator generation task and thereby reduce the effort involved in adapting to schema changes. By allowing the DBA to concentrate on identifying the modifications at a high level, instead of reprogramming the mediator, turnaround time is reduced and warehouse reliability is improved.

  14. Atomic Force Microscope Mediated Chromatography

    Science.gov (United States)

    Anderson, Mark S.

    2013-01-01

    The atomic force microscope (AFM) is used to inject a sample, provide shear-driven liquid flow over a functionalized substrate, and detect separated components. This is demonstrated using lipophilic dyes and normal phase chromatography. A significant reduction in both size and separation time scales is achieved with a 25-micron-length column scale, and one-second separation times. The approach has general applications to trace chemical and microfluidic analysis. The AFM is now a common tool for ultra-microscopy and nanotechnology. It has also been demonstrated to provide a number of microfluidic functions necessary for miniaturized chromatography. These include injection of sub-femtoliter samples, fluidic switching, and sheardriven pumping. The AFM probe tip can be used to selectively remove surface layers for subsequent microchemical analysis using infrared and tip-enhanced Raman spectroscopy. With its ability to image individual atoms, the AFM is a remarkably sensitive detector that can be used to detect separated components. These diverse functional components of microfluidic manipulation have been combined in this work to demonstrate AFM mediated chromatography. AFM mediated chromatography uses channel-less, shear-driven pumping. This is demonstrated with a thin, aluminum oxide substrate and a non-polar solvent system to separate a mixture of lipophilic dyes. In conventional chromatographic terms, this is analogous to thin-layer chromatography using normal phase alumina substrate with sheardriven pumping provided by the AFM tip-cantilever mechanism. The AFM detection of separated components is accomplished by exploiting the variation in the localized friction of the separated components. The AFM tip-cantilever provides the mechanism for producing shear-induced flows and rapid pumping. Shear-driven chromatography (SDC) is a relatively new concept that overcomes the speed and miniaturization limitations of conventional liquid chromatography. SDC is based on a

  15. Elder mediation in theory and practice: study results from a national caregiver mediation demonstration project.

    Science.gov (United States)

    Crampton, Alexandra

    2013-01-01

    Mediation is a process through which a third party facilitates discussion among disputing parties to help them identify interests and ideally reach an amicable solution. Elder mediation is a growing subspecialty to address conflicts involving older adults, primarily involving caregiving or finances. Mediation is theorized to empower participants but critics argue that it can exacerbate power imbalances among parties and coerce consensus. These contested claims are examined through study of a national caregiver mediation demonstration project. Study implications underscore the importance of gerontological social work expertise to ensure the empowerment of vulnerable older adults in mediation sessions.

  16. 7 CFR 785.5 - Fees for mediation services.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Fees for mediation services. 785.5 Section 785.5... AGRICULTURE SPECIAL PROGRAMS CERTIFIED STATE MEDIATION PROGRAM § 785.5 Fees for mediation services. A requirement that non-USDA parties who elect to participate in mediation pay a fee for mediation services...

  17. Protease-mediated drug delivery

    Science.gov (United States)

    Dickson, Eva F.; Goyan, Rebecca L.; Kennedy, James C.; Mackay, M.; Mendes, M. A. K.; Pottier, Roy H.

    2003-12-01

    Drugs used in disease treatment can cause damage to both malignant and normal tissue. This toxicity limits the maximum therapeutic dose. Drug targeting is of high interest to increase the therapeutic efficacy of the drug without increasing systemic toxicity. Certain tissue abnormalities, disease processes, cancers, and infections are characterized by high levels of activity of specific extracellular and/or intracellular proteases. Abnormally high activity levels of specific proteases are present at sites of physical or chemical trauma, blood clots, malignant tumors, rheumatoid arthritis, inflammatory bowel disease, gingival disease, glomerulonerphritis, and acute pancreatitis. Abnormal protease activity is suspected in development of liver thrombosis, pulmonary emphysema, atherosclerosis, and muscular dystrophy. Inactiviating disease-associated proteases by the administration of appropriate protease inhibitors has had limited success. Instead, one could use such proteases to target drugs to treat the condition. Protease mediated drug delivery offers such a possibility. Solubilizing groups are attached to insoluble drugs via a polypeptide chain which is specifically cleavable by certian proteases. When the solubilized drug enounters the protease, the solubilizing moieties are cleaved, and the drug precipitates at the disease location. Thus, a smaller systemic dosage could result in a therapeutic drug concentration at the treatment site with less systemic toxicity.

  18. Gauge mediation with light stops

    CERN Document Server

    Delgado, Antonio; Quiros, Mariano

    2015-01-01

    The mechanism of gauge mediated supersymmetry breaking (GMSB) solves the supersymmetric flavor problem although it requires superheavy stops to reproduce the experimental value (125 GeV) of the Higgs mass. A possible way out is to extend the MSSM Higgs sector with triplets which provide extra tree-level corrections to the Higgs mass. Triplets with neutral components getting vacuum expectation values (VEV) have the problem of generating a tree-level correction to the \\rho parameter. We introduce supersymmetric triplets with hypercharges Y=(0,\\pm 1), with a tree-level custodial SU(2)_L\\otimes SU(2)_R global symmetry in the Higgs sector protecting the \\rho parameter: a supersymmetric generalization of the Georgi-Machacek model. The renormalization group running from the messenger to the electroweak scale mildly breaks the custodial symmetry. We will present realistic low-scale scenarios, their main features being a Bino-like neutralino or right-handed stau as the NLSP, light (1 TeV) stops, exotic couplings (H^\\p...

  19. Immunomodulatory Effects Mediated by Dopamine

    Science.gov (United States)

    Alvarez-Herrera, Samantha; Pérez-Sánchez, Gilberto; Becerril-Villanueva, Enrique; Cruz-Fuentes, Carlos; Flores-Gutierrez, Enrique Octavio; Quintero-Fabián, Saray

    2016-01-01

    Dopamine (DA), a neurotransmitter in the central nervous system (CNS), has modulatory functions at the systemic level. The peripheral and central nervous systems have independent dopaminergic system (DAS) that share mechanisms and molecular machinery. In the past century, experimental evidence has accumulated on the proteins knowledge that is involved in the synthesis, reuptake, and transportation of DA in leukocytes and the differential expression of the D1-like (D1R and D5R) and D2-like receptors (D2R, D3R, and D4R). The expression of these components depends on the state of cellular activation and the concentration and time of exposure to DA. Receptors that are expressed in leukocytes are linked to signaling pathways that are mediated by changes in cAMP concentration, which in turn triggers changes in phenotype and cellular function. According to the leukocyte lineage, the effects of DA are associated with such processes as respiratory burst, cytokine and antibody secretion, chemotaxis, apoptosis, and cytotoxicity. In clinical conditions such as schizophrenia, Parkinson disease, Tourette syndrome, and multiple sclerosis (MS), there are evident alterations during immune responses in leukocytes, in which changes in DA receptor density have been observed. Several groups have proposed that these findings are useful in establishing clinical status and clinical markers. PMID:27795960

  20. Immunomodulatory Effects Mediated by Dopamine

    Directory of Open Access Journals (Sweden)

    Rodrigo Arreola

    2016-01-01

    Full Text Available Dopamine (DA, a neurotransmitter in the central nervous system (CNS, has modulatory functions at the systemic level. The peripheral and central nervous systems have independent dopaminergic system (DAS that share mechanisms and molecular machinery. In the past century, experimental evidence has accumulated on the proteins knowledge that is involved in the synthesis, reuptake, and transportation of DA in leukocytes and the differential expression of the D1-like (D1R and D5R and D2-like receptors (D2R, D3R, and D4R. The expression of these components depends on the state of cellular activation and the concentration and time of exposure to DA. Receptors that are expressed in leukocytes are linked to signaling pathways that are mediated by changes in cAMP concentration, which in turn triggers changes in phenotype and cellular function. According to the leukocyte lineage, the effects of DA are associated with such processes as respiratory burst, cytokine and antibody secretion, chemotaxis, apoptosis, and cytotoxicity. In clinical conditions such as schizophrenia, Parkinson disease, Tourette syndrome, and multiple sclerosis (MS, there are evident alterations during immune responses in leukocytes, in which changes in DA receptor density have been observed. Several groups have proposed that these findings are useful in establishing clinical status and clinical markers.

  1. Towards the Natural Gauge Mediation

    CERN Document Server

    Ding, Ran; Wang, Liucheng; Zhu, Bin

    2015-01-01

    The sweet spot supersymmetry (SUSY) solves the mu problem in the Minimal Supersymmetric Standard Model (MSSM) with gauge mediated SUSY breaking (GMSB) via the generalized Giudice-Masiero (GM) mechanism where only the mu-term and soft Higgs masses are generated at the unification scale of the Grand Unified Theory (GUT) due to the approximate PQ symmetry. Because all the other SUSY breaking soft terms are generated via the GMSB below the GUT scale, there exists SUSY electroweak (EW) fine-tuning problem to explain the 125 GeV Higgs boson mass due to small trilinear soft term. Thus, to explain the Higgs boson mass, we propose the GMSB with both the generalized GM mechanism and Higgs-messenger interactions. The renormalization group equations are runnings from the GUT scale down to EW scale. So the EW symmetry breaking can be realized easier. We can keep the gauge coupling unification and solution to the flavor problem in the GMSB, as well as solve the \\mu/B_{\\mu}-problem. Moreover, there are only five free parame...

  2. 76 FR 19310 - Information Collection; Certified State Mediation Program

    Science.gov (United States)

    2011-04-07

    ... Farm Service Agency Information Collection; Certified State Mediation Program AGENCY: Farm Service... Mediation Program. The information collection is necessary to ensure the grant program is being administered... following methods: Mail: Carol Wagner, Certified State Mediation Program Manager, USDA, FSA, Appeals...

  3. [Role of internal mediators in surgical stress].

    Science.gov (United States)

    Endo, Shigeatsu; Sato, Nobuhiro

    2003-12-01

    In surgical stress, internal mediators include cytokines released locally as well as into the circulation; activation of neutrophils, monocytes, macrophages, endothelial cells, platelets and other cells; activation of plasma protein cascade systems such the complement, coagulation, fibrinolytic and contact systems the release of tissue-damaging proteinases; the formation of lipid mediators such as eicosanoids and platelet-activating factor; and the generation of oxygen and nitrogen radicals. In addition, during host defense rections, a number of agents are released that blunt the inflammatory response, such as anti-inflamatory cytokines, soluble cytokine receptors, acute-phase proteins including proteinase inhibitors and stress hormones. In this paper, we explain such internal mediators.

  4. Digital methods for mediated discourse analysis

    DEFF Research Database (Denmark)

    Kjær, Malene; Larsen, Malene Charlotte

    2015-01-01

    , restrictions or privately mediated settings. Having used mediated discourse analysis (Scollon 2002, Scollon & Scollon, 2004) as a framework in two different research projects, we show how the framework, in correlation with digital resources for data gathering, provides new understandings of 1) the daily...... practice of health care professionals (Author 1, 2014) and 2) young people’s identity construction on social media platforms (Author 2, 2010, 2015, in press). The paper’s contribution is a methodological discussion on digital data collection using methods such as online interviewing (via e-mail or chat......) and online questionnaire data in order to capture mediated actions and discourses in practice....

  5. Current rectification by mediating electroactive polymers

    Energy Technology Data Exchange (ETDEWEB)

    Ybarra, Gabriel; Moina, Carlos [Centro de Investigacion sobre Electrodeposicion y Procesos Superficiales, Instituto Nacional de Tecnologia Industrial, CC 157, (1650) San Martin (Argentina); Florit, M. Ines [INIFTA, Facultad de Ciencias Exactas, UNLP, Suc. 4, CC 16, (1900) La Plata (Argentina); Posadas, Dionisio [INIFTA, Facultad de Ciencias Exactas, UNLP, Suc. 4, CC 16, (1900) La Plata (Argentina)], E-mail: dposadas@inifta.unlp.edu.ar

    2008-04-20

    In this work we briefly review the theoretical basis for the electrochemical rectification in mediated redox reactions at redox polymer modified electrodes. Electrochemical rectification may have two distinct origins. It is either caused by a slow kinetics of the reaction between the external redox couple and the mediator or it is originated by a slow electronic transport within the film under an unfavorable thermodynamic condition. We show experimental results for the redox mediation reaction of poly(o-aminophenol) (POAP) on the Fe{sup 2+/3+} and on the Fe(CN){sub 6}{sup 3-/4-} redox couples in solution that prove the proposed mechanisms of electrochemical rectification.

  6. Singlet deflected anomaly/gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Blas, J. de, E-mail: jdeblasm@nd.edu [Department of Physics, University of Notre Dame, Notre Dame, IN 46556 (United States); Delgado, A., E-mail: antonio.delgado@nd.edu [Department of Physics, University of Notre Dame, Notre Dame, IN 46556 (United States)

    2012-02-28

    We study an extension of the standard anomaly/gauge mediation scenario where the messenger fields have direct interactions with an extra gauge singlet. This realizes a phenomenologically viable NMSSM-like scenario free of the {mu}-b{sub {mu}} problem. Current cosmological constraints imply a small size for the anomaly-mediation contributions, unless some source of R-parity violation is permitted. In the latter case the allowed regions in the parameter space can be substantially larger than in the corresponding gauge-mediation scenario.

  7. CELLULAR INTERACTIONS MEDIATED BY GLYCONECTIDS

    Directory of Open Access Journals (Sweden)

    O.Popescu

    1999-01-01

    Full Text Available Cellular interactions involve many types of cell surface molecules and operate via homophilic and/or heterophilic protein-protein and protein-carbohydrate binding. Our investigations in different model-systems (marine invertebrates and mammals have provided direct evidence that a novel class of primordial proteoglycans, named by us gliconectins, can mediate cell adhesion via a new alternative molecular mechanism of polyvalent carbohydrate-carbohydrate binding. Biochemical characterization of isolated and purified glyconectins revealed the presence of specific carbohydrate structures, acidic glycans, different from classical glycosaminoglycans. Such acidic glycans of high molecular weight containing fucose, glucuronic or galacturonic acids, and sulfate groups, originally found in sponges and sea urchin embryos, may represent a new class of carbohydrate carcino-embryonal antigens in mice and humans. Such interactions between biological macromolecules are usually investigated by kinetic binding studies, calorimetric methods, X-ray diffraction, nuclear magnetic resonance, and other spectroscopic analyses. However, these methods do not supply a direct estimation of the intermolecular binding forces that are fundamental for the function of the ligand-receptor association. Recently, we have introduced atomic force microscopy to quantify the binding strength between cell adhesion proteoglycans. Measurement of binding forces intrinsic to cell adhesion proteoglycans is necessary to assess their contribution to the maintenance of the anatomical integrity of multicellular organisms. As a model, we selected the glyconectin 1, a cell adhesion proteoglycan isolated from the marine sponge Microciona prolifera. This glyconectin mediates in vivo cell recognition and aggregation via homophilic, species-specific, polyvalent, and calcium ion-dependent carbohydrate-carbohydrate interactions. Under physiological conditions, an adhesive force of up to 400 piconewtons

  8. Electroporation-mediated gene delivery.

    Science.gov (United States)

    Young, Jennifer L; Dean, David A

    2015-01-01

    Electroporation has been used extensively to transfer DNA to bacteria, yeast, and mammalian cells in culture for the past 30 years. Over this time, numerous advances have been made, from using fields to facilitate cell fusion, delivery of chemotherapeutic drugs to cells and tissues, and most importantly, gene and drug delivery in living tissues from rodents to man. Electroporation uses electrical fields to transiently destabilize the membrane allowing the entry of normally impermeable macromolecules into the cytoplasm. Surprisingly, at the appropriate field strengths, the application of these fields to tissues results in little, if any, damage or trauma. Indeed, electroporation has even been used successfully in human trials for gene delivery for the treatment of tumors and for vaccine development. Electroporation can lead to between 100 and 1000-fold increases in gene delivery and expression and can also increase both the distribution of cells taking up and expressing the DNA as well as the absolute amount of gene product per cell (likely due to increased delivery of plasmids into each cell). Effective electroporation depends on electric field parameters, electrode design, the tissues and cells being targeted, and the plasmids that are being transferred themselves. Most importantly, there is no single combination of these variables that leads to greatest efficacy in every situation; optimization is required in every new setting. Electroporation-mediated in vivo gene delivery has proven highly effective in vaccine production, transgene expression, enzyme replacement, and control of a variety of cancers. Almost any tissue can be targeted with electroporation, including muscle, skin, heart, liver, lung, and vasculature. This chapter will provide an overview of the theory of electroporation for the delivery of DNA both in individual cells and in tissues and its application for in vivo gene delivery in a number of animal models.

  9. Towards the natural gauge mediation

    Science.gov (United States)

    Ding, Ran; Li, Tianjun; Wang, Liucheng; Zhu, Bin

    2015-10-01

    The sweet spot supersymmetry (SUSY) solves the μ/ B μ problem in the Minimal Supersymmetric Standard Model (MSSM) with gauge mediated SUSY breaking (GMSB) via the generalized Giudice-Masiero (GM) mechanism where only the μ-term and soft Higgs masses are generated at the unification scale of the Grand Unified Theory (GUT) due to the approximate PQ symmetry. Because all the other SUSY breaking soft terms are generated via the GMSB below the GUT scale, there exists SUSY electroweak (EW) fine-tuning problem to explain the 125 GeV Higgs boson mass due to small trilinear soft term. Thus, to explain the Higgs boson mass, we propose the GMSB with both the generalized GM mechanism and Higgs-messenger interactions. The renormalization group equations are runnings from the GUT scale down to EW scale. So the EW symmetry breaking can be realized easier. We can keep the gauge coupling unification and solution to the flavor problem in the GMSB, as well as solve the μ/ B μ -problem. Moreover, there are only five free parameters in our model. So we can determine the characteristic low energy spectra and explore its distinct phenomenology. The fine-tuning measure can be as low as 100. For some benchmark points, the stop mass can be as low as 1.7 TeV while the glunio mass is around 2.5 TeV. The gravitino dark matter can come from a thermal production with the correct relic density and be consistent with the thermal leptogenesis. Because gluino and stop can be relatively light in our model, how to search for such GMSB at the upcoming run II of the LHC experiment could be very interesting.

  10. Upscaling of Bio-mediated Soil Improvement

    Energy Technology Data Exchange (ETDEWEB)

    J. T. DeJong; B. C. Martinez; B. M. Mortensen; D. C. Nelson; J. T. Waller; M. H. Weil; T. R. Ginn; T. Weathers; T. Barkouki; Y. Fujita; G. Redden; C. Hunt; D. Major; B. Tunyu

    2009-10-01

    As demand for soil improvement continues to increase, new, sustainable, and innocuous methods are needed to alter the mechanical properties of soils. Recent research has demonstrated the potential of bio-mediated soil improvement for geotechnical applications (DeJong et al. 2006, Whiffin et al. 2007). Upscaling the bio-mediated treatment process for in situ implementation presents a number of challenges to be addressed, including soil and pore fluid interactions, bioaugmentation versus biostimulation of microbial communities, controlled distribution of mediated calcite precipitation, and permanence of the cementation. Current studies are utilizing large-scale laboratory experiments, non-destructive geophysical measurements, and modeling, to develop an optimized and predictable bio-mediated treatment method.

  11. Genesis and intersubjectivity: levels of mediation.

    Science.gov (United States)

    Yamamoto, Toshiya

    2012-09-01

    I will make a brief comment on the thesis of José C. Loredo-Narciandi and José C. Sánchez-González (2012) "Neither Dichotomies Nor Dualism; Simply Genesis". Denying any reductionism and reification, they insists the importance of inseparable relation between subject and object, then discuss about genesis. I pointed out that their argument lacks an important element, i.e., mediator that differ from mere physical object. After discussing mediators nature, I discriminate the three level of interaction which mediated by mediator. The first is the pre-interaction level, the second is physical interaction level and the third is sign or language interaction level. The last argument of mine is about how we can solve the problem of reification and reductionism. In the argument it is pointed out that intersubjectivity is key concept for understanding and solving the problem appropriately.

  12. Cybercultures mediations of community, culture, politics

    CERN Document Server

    Harris, Breslow

    2012-01-01

    Cybercultures: Mediations of Community, Culture, Politics , is a collection of essays that critically examine the role that digital media and online cultures play in the rearticulation of contemporary societies, cultures and polities. This volume in

  13. Programmable DNA-mediated multitasking processor

    CERN Document Server

    Shu, Jian-Jun; Yong, Kian-Yan; Shao, Fangwei; Lee, Kee Jin

    2015-01-01

    Because of DNA appealing features as perfect material, including minuscule size, defined structural repeat and rigidity, programmable DNA-mediated processing is a promising computing paradigm, which employs DNAs as information storing and processing substrates to tackle the computational problems. The massive parallelism of DNA hybridization exhibits transcendent potential to improve multitasking capabilities and yield a tremendous speed-up over the conventional electronic processors with stepwise signal cascade. As an example of multitasking capability, we present an in vitro programmable DNA-mediated optimal route planning processor as a functional unit embedded in contemporary navigation systems. The novel programmable DNA-mediated processor has several advantages over the existing silicon-mediated methods, such as conducting massive data storage and simultaneous processing via much fewer materials than conventional silicon devices.

  14. Dark matter prospects in deflected mirage mediation

    Energy Technology Data Exchange (ETDEWEB)

    Holmes, Michael; Nelson, Brent D., E-mail: holmes.mi@neu.edu, E-mail: b.nelson@neu.edu [Department of Physics, Northeastern University, Boston, MA 02115 (United States)

    2009-07-01

    The recently introduced deflected mirage mediation (DMM) model is a string-motivated paradigm in which all three of the major supersymmetry-breaking transmission mechanisms are operative. We begin a systematic exploration of the parameter space of this rich model context, paying special attention to the pattern of gaugino masses which arise. In this work we focus on the dark matter phenomenology of the DMM model as such signals are the least influenced by the model-dependent scalar masses. We find that a large portion of the parameter space in which the three mediation mechanisms have a similar effective mass scale of 1 TeV or less will be probed by future direct and indirect detection experiments. Distinguishing deflected mirage mediation from the mirage model without gauge mediation will prove difficult without collider input, though we indicate how gamma ray signals may provide an opportunity for distinguishing between the two paradigms.

  15. Nordic Mediation - Comparing Denmark and Finland

    DEFF Research Database (Denmark)

    Lappi-Seppälä, Tapio; Storgaard, Anette

    2015-01-01

    The Nordic Countries have a long common history in criminal policy but a closer look also indicates individual Development. the introduction of Victim Offender Mediation is one example of Nordic diversity in details.......The Nordic Countries have a long common history in criminal policy but a closer look also indicates individual Development. the introduction of Victim Offender Mediation is one example of Nordic diversity in details....

  16. Gazetteer Brokering through Semantic Mediation

    Science.gov (United States)

    Hobona, G.; Bermudez, L. E.; Brackin, R.

    2013-12-01

    web technologies, including ontologies and a semantic mediator. The semantically-enhanced SPEGG allowed a client to submit a single query (e.g. ';hills') and to retrieve data from two separate gazetteers with different vocabularies (e.g. where one refers to ';summits' another refers to ';hills'). Supporting the SPEGG was a SPARQL server that held the ontologies and processed queries on them. Earth Science surveys and forecast always have a place on Earth. Being able to share the information about a place and solve inconsistencies about that place from different sources will enable geoscientists to better do their research. In the advent of mobile geo computing and location based services (LBS), brokering gazetteers will provide geoscientists with access to gazetteer services rich with information and functionality beyond that offered by current generic gazetteers.

  17. Lipid Mediators in the Resolution of Inflammation

    Science.gov (United States)

    Serhan, Charles N.; Chiang, Nan; Dalli, Jesmond; Levy, Bruce D.

    2015-01-01

    Mounting of the acute inflammatory response is crucial for host defense and pivotal to the development of chronic inflammation, fibrosis, or abscess formation versus the protective response and the need of the host tissues to return to homeostasis. Within self-limited acute inflammatory exudates, novel families of lipid mediators are identified, named resolvins (Rv), protectins, and maresins, which actively stimulate cardinal signs of resolution, namely, cessation of leukocytic infiltration, counterregulation of proinflammatory mediators, and the uptake of apoptotic neutrophils and cellular debris. The biosynthesis of these resolution-phase mediators in sensu stricto is initiated during lipid-mediator class switching, in which the classic initiators of acute inflammation, prostaglandins and leukotrienes (LTs), switch to produce specialized proresolving mediators (SPMs). In this work, we review recent evidence on the structure and functional roles of these novel lipid mediators of resolution. Together, these show that leukocyte trafficking and temporal spatial signals govern the resolution of self-limited inflammation and stimulate homeostasis. PMID:25359497

  18. Ombuds’ corner: Consider mediation - some rules

    CERN Multimedia

    Vincent Vuillemin

    2013-01-01

    Mediation is a structured process in which an external party, called a mediator, helps participants generate and evaluate options that would allow them to reach a mutual agreement. It is an informal and confidential process.   The mediator does not have the power to impose an agreement on the parties, who should find it by themselves. However, the mediator controls the process. He arranges the meetings in agreement with the parties, and coordinates the details (concerning the speaking times, for example) and ensures that the parties respect of the rules which have been agreed on. He also favours advancing of the process towards a solution. The mediation process is centred on the search for a solution and a mutual agreement. In such a process, the mediator takes a neutral and impartial position, and does not advocate for a single party. He favours good communication between the parties, and will ensure that mutual respect is maintained and that the correct language is used during the discussi...

  19. Assessing moderated mediation in linear models requires fewer confounding assumptions than assessing mediation.

    Science.gov (United States)

    Loeys, Tom; Talloen, Wouter; Goubert, Liesbet; Moerkerke, Beatrijs; Vansteelandt, Stijn

    2016-11-01

    It is well known from the mediation analysis literature that the identification of direct and indirect effects relies on strong no unmeasured confounding assumptions of no unmeasured confounding. Even in randomized studies the mediator may still be correlated with unobserved prognostic variables that affect the outcome, in which case the mediator's role in the causal process may not be inferred without bias. In the behavioural and social science literature very little attention has been given so far to the causal assumptions required for moderated mediation analysis. In this paper we focus on the index for moderated mediation, which measures by how much the mediated effect is larger or smaller for varying levels of the moderator. We show that in linear models this index can be estimated without bias in the presence of unmeasured common causes of the moderator, mediator and outcome under certain conditions. Importantly, one can thus use the test for moderated mediation to support evidence for mediation under less stringent confounding conditions. We illustrate our findings with data from a randomized experiment assessing the impact of being primed with social deception upon observer responses to others' pain, and from an observational study of individuals who ended a romantic relationship assessing the effect of attachment anxiety during the relationship on mental distress 2 years after the break-up.

  20. Psycho-pedagogical peculiarities of mediator`s training: comparative analysis

    Directory of Open Access Journals (Sweden)

    Urusova Olena

    2016-09-01

    Full Text Available The article deals with psychological and pedagogical features of Ukrainian and foreign training programs for mediators. The comparative analysis allowed to determine that the training programs for future mediators are focused on the development of practical skills using interactive forms of learning, however they need to be improved in terms of establishment of professional self-identity, as well as a deeper self-reflection and self-knowledge of the participants. It was determined that the existing best practice of training programs for mediators may be used while developing the program of generation of psychological readiness of the future mediators for professional activities.

  1. Organizational mediators siding with or against the powerful party

    NARCIS (Netherlands)

    Laskewitz, P; Van de Vliert, E; De Dreu, C K W

    1994-01-01

    Instead of being impartial, a mediator may side with one party as a function of the disputants' power differences, the mediator's legitimacy judgments, or the disputants' capacity to sanction the mediator. According to the power balancing theory, a mediator sides with the party that has a power disa

  2. 15 CFR 930.115 - Termination of mediation.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Termination of mediation. 930.115... MANAGEMENT FEDERAL CONSISTENCY WITH APPROVED COASTAL MANAGEMENT PROGRAMS Secretarial Mediation § 930.115 Termination of mediation. Mediation shall terminate: (a) At any time the Federal and State agencies agree to...

  3. Surveying Parental Mediation: Connections, Challenges and Questions for Media Literacy

    Science.gov (United States)

    Mendoza, Kelly

    2009-01-01

    This paper examines three strategies of parental mediation--coviewing, restrictive mediation, and active mediation--in order to make connections, challenge, and raise questions for media literacy. Coviewing, whether it is intentional practice, or whether it functions to promote media literacy, is explored. Restrictive mediation, how it connects to…

  4. 15 CFR 923.83 - Mediation of amendments.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Mediation of amendments. 923.83... Programs § 923.83 Mediation of amendments. (a) Section 307(h)(2) of the Act provides for mediation of... management program. Accordingly mediation is available to states or federal agencies when a...

  5. 29 CFR 1207.2 - Requests for Mediation Board action.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Requests for Mediation Board action. 1207.2 Section 1207.2 Labor Regulations Relating to Labor (Continued) NATIONAL MEDIATION BOARD ESTABLISHMENT OF SPECIAL ADJUSTMENT BOARDS § 1207.2 Requests for Mediation Board action. (a) Requests for the National Mediation...

  6. A State Space Modeling Approach to Mediation Analysis

    Science.gov (United States)

    Gu, Fei; Preacher, Kristopher J.; Ferrer, Emilio

    2014-01-01

    Mediation is a causal process that evolves over time. Thus, a study of mediation requires data collected throughout the process. However, most applications of mediation analysis use cross-sectional rather than longitudinal data. Another implicit assumption commonly made in longitudinal designs for mediation analysis is that the same mediation…

  7. Estimating and Testing Mediation Effects with Censored Data

    Science.gov (United States)

    Wang, Lijuan; Zhang, Zhiyong

    2011-01-01

    This study investigated influences of censored data on mediation analysis. Mediation effect estimates can be biased and inefficient with censoring on any one of the input, mediation, and output variables. A Bayesian Tobit approach was introduced to estimate and test mediation effects with censored data. Simulation results showed that the Bayesian…

  8. Construction of Mediators for Heterogeneous Data Source Integration Systems

    Institute of Scientific and Technical Information of China (English)

    高明; 宋瀚涛

    2003-01-01

    To construct mediators for data integration systems that integrate structured and semi-structured data, and to facilitate the reformulation and decomposition of the query, the presented system uses the XML processing language (XPL) for the mediator. With XPL, it is easy to construct mediators for data integration based on XML, and it can accelerate the work in the mediator.

  9. Pure Mediated Priming: A Retrospective Semantic Matching Model

    Science.gov (United States)

    Jones, Lara L.

    2010-01-01

    Mediated priming refers to the activation of a target (e.g., "stripes") by a prime (e.g., "lion") that is related indirectly via a connecting mediator (e.g., tiger). In previous mediated priming studies (e.g., McNamara & Altarriba, 1988), the mediator was associatively related to the prime. In contrast, pure mediated…

  10. An exponential decay model for mediation.

    Science.gov (United States)

    Fritz, Matthew S

    2014-10-01

    Mediation analysis is often used to investigate mechanisms of change in prevention research. Results finding mediation are strengthened when longitudinal data are used because of the need for temporal precedence. Current longitudinal mediation models have focused mainly on linear change, but many variables in prevention change nonlinearly across time. The most common solution to nonlinearity is to add a quadratic term to the linear model, but this can lead to the use of the quadratic function to explain all nonlinearity, regardless of theory and the characteristics of the variables in the model. The current study describes the problems that arise when quadratic functions are used to describe all nonlinearity and how the use of nonlinear functions, such as exponential decay, address many of these problems. In addition, nonlinear models provide several advantages over polynomial models including usefulness of parameters, parsimony, and generalizability. The effects of using nonlinear functions for mediation analysis are then discussed and a nonlinear growth curve model for mediation is presented. An empirical example using data from a randomized intervention study is then provided to illustrate the estimation and interpretation of the model. Implications, limitations, and future directions are also discussed.

  11. LITEROMUSICAL LITERACY: SOCIAL PRACTICES MEDIATED BY SONGS

    Directory of Open Access Journals (Sweden)

    Jose Peixoto Coelho de Souza

    2015-10-01

    Full Text Available Based on the assumption that songs are a speech genre consisting of both music and lyrics (COSTA, 2002; COELHO DE SOUZA, 2010; CARETTA, 2011 and that, consequently, its meanings derive from the articulation between both languages, this paper aims to introduce the concept of literomusical literacy, i.e., the literacy involved in social practices mediated by songs and verbo-musical genres. Grounded on the concept of literacy as social practices mediated by written language (STREET, 1984, 2006; KLEIMAN, 1995; SOARES, 1999, 2002; BARTON, 2007 and bringing contributions from studies on literary literacy (PAULINO, 2004; COSSON, 2006; PAULINO; COSSON, 2009 and music education (SWANWICK, 1994, 2003; FRANÇA; SWANWICK, 2002, literomusical literacy is conceived as the state or condition of those who participate in social practices mediated by songs and discourses that emerge from songs and take a critical stand on them because they are able to understand and reflect upon their verbal and musical components, on how they articulate to build certain meaning effects and on how they relate to their musical community. This involves, for instance, recognizing and interpreting the actions that are being mediated by songs, and through this interpretation, to be able to understand the values underlying them and the target interlocutors. Acknowledging the existence of a particular literacy involved in the social practices mediated by verbo-musical genres entails reflecting on the pedagogical practices associated with the use of songs in language teaching and how to turn these literacy practices into literomusical literacy practices.

  12. Surmounting elusive barriers: the case for bioethics mediation.

    Science.gov (United States)

    Bergman, Edward J

    2013-01-01

    This article describes, analyzes, and advocates for management of clinical healthcare conflict by a process commonly referred to as bioethics mediation. Section I provides a brief introduction to classical mediation outside the realm of clinical healthcare. Section II highlights certain distinguishing characteristics of bioethics mediation. Section III chronicles the history of bioethics mediation and references a number of seminal writings on the subject. Finally, Section IV analyzes barriers that have, thus far, limited the widespread implementation of bioethics mediation.

  13. Sensemaking in Technology-Use Mediation

    DEFF Research Database (Denmark)

    Bansler, Jørgen P.; Havn, Erling C.

    2006-01-01

    of advanced CSCW technologies is basically a problem of sensemaking. We analyze how a group of “technology-use mediators” (Orlikowski et al. Org. Sci. (1995) 6(4), 423) in a large, multinational company adapted a groupware technology (a “virtual workspace”) to the local organizational context (and vice versa......) by modifying features of the technology, providing ongoing support for users, and promoting appropriate conventions of use. Our findings corroborate earlier research on technology-use mediation, which suggests that such mediators can exert considerable influence on how a particular technology...... will be established and used in an organization. However, we also find that the process of technology-use mediation is much more complex and indeterminate than prior research suggests. The reason being, we argue, that new, advanced CSCW technologies, such as “virtual workspaces” and other groupware applications...

  14. Revisiting R-invariant Direct Gauge Mediation

    CERN Document Server

    Chiang, Cheng-Wei; Ibe, Masahiro; Yanagida, Tsutomu T

    2015-01-01

    We revisit a special model of gauge mediated supersymmetry breaking, the "R-invariant direct gauge mediation." We pay particular attention to whether the model is consistent with the minimal model of the \\mu-term, i.e., a simple mass term of the Higgs doublets in the superpotential. Although the incompatibility is highlighted in view of the current experimental constraints on the superparticle masses and the observed Higgs boson mass, the minimal \\mu-term can be consistent with the R-invariant gauge mediation model via a careful choice of model parameters. We derive an upper limit on the gluino mass from the observed Higgs boson mass. We also discuss whether the model can explain the 3\\sigma excess of the Z+jets+$E_T^{\\rm miss}$ events reported by the ATLAS Collaboration.

  15. Agrobacterium-mediated transformation of cotton.

    Science.gov (United States)

    Zhang, Baohong

    2013-01-01

    There are many methods and techniques that can be used to transfer foreign genes into cells. In plant biotechnology, Agrobacterium-mediated transformation is a widely used traditional method for inserting foreign genes into plant genome and obtaining transgenic plants, particularly for dicot plant species. Agrobacterium-mediated transformation of cotton involves several important and also critical steps, which includes coculture of cotton explants with Agrobacterium, induction and selection of stable transgenic cell lines, recovery of plants from transgenic cells majorly through somatic embryogenesis, and detection and expression analysis of transgenic plants. In this chapter, we describe a detailed step-by-step protocol for obtaining transgenic cotton plants via Agrobacterium-mediated transformation.

  16. Criminal Mediation for Minors in Israel

    Directory of Open Access Journals (Sweden)

    Isack Kandel

    2007-01-01

    Full Text Available Mediation was introduced in Canada in 1974 in order to handle a crime of robbery and vandalization committed by adolescents. After mediation, these adolescents agreed to apologize to each of their victims and pay restitution. Several countries (Canada, England, Finland, and the U.S. have now made this opportunity available in the cases of young offenders. This review describes the process in the south of Israel. We find this method very powerful, but further studies are needed. Due to resource problems, it will not become mainstream in the near future.

  17. Cosmological aspects of gauge mediated supersymmetry breakdown

    CERN Document Server

    Dalianis, Ioannis

    2011-01-01

    In this thesis, we study the details of some fundamental cosmological problems of the gauge mediated supersymmetry breakdown and we probe the supersymmetry breaking sector by cosmological arguments. We manifest that problems like the metastable vacuum selection and the gravitino overproduction in the reheated early universe are naturally absent in the most general class of gauge mediation models without including additional ingredients or assumptions. We also find that the gravitino can generically account for the bulk dark matter of the universe. Cosmological implications of a stringy UV-completion of the supersymmetry breaking sector have been also considered.

  18. Instrumentness for Creativity - Mediation, Materiality & Metonymy

    DEFF Research Database (Denmark)

    Bertelsen, Olav Wedege; Breinbjerg, Morten; Pold, Søren

    2007-01-01

    We introduce the concept instrumentness as a quality of human-computer interfaces. Instrumentness points to the way musical instruments are controlled and conceptualized through values such as virtuosity and playability, which are important for computer-mediated creative work supporting development...... as central strategies for computer mediated creativity. The paper is contributing to an investigation of the aesthetics of use in relation to software, pointing to alternative values, differing from traditional usability, which are also relevant in creative work outside art and music composition....

  19. The Maieutic Force of Mediating Instruments

    DEFF Research Database (Denmark)

    Revellino, Silvana; Mouritsen, Jan

    and coordinating complex investments in inter-organizational spaces, the paper explores the role of the Chronoprogram in managing compromises and directing attention to concerns in relation to the development of an Italian piece of motorway(the Variante di Valico).The Chronoprogram acts as a mediating instrument...... transform actions in unexpected directions which reconfigure the capital budgeting complex investment project and also extend the motorway object. The paper thus further develops the notion of mediating instruments by highlighting their maieutic ability to address the dialectics between matters of fact...

  20. Purely Four-dimensional Viable Anomaly Mediation

    CERN Document Server

    Harnik, R; Pierce, A T; Harnik, Roni; Murayama, Hitoshi; Pierce, Aaron

    2002-01-01

    Anomaly mediation of supersymmetry breaking solves the supersymmetric flavor problem thanks to its ultraviolet-insensitivity. However, it suffers from two problems: sleptons have negative masses-squared, and there are likely bulk moduli that spoil the framework. Here, we present the first fully ultraviolet-insensitive model of anomaly mediation with positive slepton masses-squared in a purely four-dimensional framework. Our model is based on the additional D-term contributions to the sparticle masses of Arkani-Hamed, Kaplan, HM, and Nomura, and the conformal sequestering mechanism of Luty and Sundrum.

  1. Extended tree-level gauge mediation

    DEFF Research Database (Denmark)

    Monaco, M.; Nardecchia, M.; Romanino, A.;

    2011-01-01

    Tree-level gauge mediation (TGM) is a scenario of SUSY breaking in which the tree-level exchange of heavy (possibly GUT) vector fields generates flavor-universal sfermion masses. In this work we extend this framework to the case of E(6) that is the natural extension of the minimal case studied so...... if the gauge group does not contain SU(5). If SUSY breaking is mediated purely by the U(1) generator that commutes with SO(10) we obtain universal sfermion masses and thus can derive the CMSSM boundary conditions in a novel scenario....

  2. A simple model of direct gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Zheng Sibo, E-mail: sibozheng.zju@gmail.com [Department of Physics, Chongqing University, Chongqing 401331 (China); Yu Yao [Department of Physics, Chongqing University, Chongqing 401331 (China)

    2012-03-23

    In the context of direct gauge mediation Wess-Zumino models are very attractive in supersymmetry model building. Besides the spontaneous supersymmetry and R-symmetry breaking, the problems of small gaugino mass as well as {mu} and B{mu} terms should be solved so as to achieve a viable model. In this Letter, we propose a simple model as an existence proof, in which all these subjects are realized simultaneously, with no need of fine tuning. This completion also implies that much of parameter space for direct gauge mediation can be directly explored at LHC.

  3. Transition to whistler mediated magnetic reconnection

    Science.gov (United States)

    Mandt, M. E.; Denton, R. E.; Drake, J. F.

    1994-01-01

    The transition in the magnetic reconnection rate from the resistive magnetohydrodynamic (MHD) regime where the Alfen wave controls reconnection to a regime in which the ions become unmagnetized and the whistler wave mediates reconnection is explored with 2-D hybrid simulations. In the whistler regime the electrons carry the currents while the ions provide a neutralizing background. A simple physical picture is presented illustrating the role of the whistler mediated reconnection is calculated analytically. The development of an out-of-plane component of the magnetic field is an observable signature of whistler driven reconnection.

  4. Lipid Mediators and Human Leukemic Blasts

    Directory of Open Access Journals (Sweden)

    Rémi Fiancette

    2011-01-01

    Full Text Available Some of the most potent inflammatory mediators share a lipid origin. They regulate a wide spectrum of cellular processes including cell proliferation and apoptosis. However, the precise roles and ways (if any in which these compounds impact the growth and apoptosis of leukemic blasts remain incompletely resolved. In spite of this, significant advances have been recently made. Here we briefly review the current knowledge about the production of lipid mediators (prostaglandins, leukotrienes, platelet-activating factor by leukemic blasts, the enzymatic activities (phospholipase A2, cyclooxygenases, lipoxygenases involved in their productions and their effects (through specific membrane bound receptors on the growth, and apoptosis of leukemic blasts.

  5. Flexible Mediation Analysis in the Presence of Nonlinear Relations: Beyond the Mediation Formula.

    Science.gov (United States)

    Loeys, Tom; Moerkerke, Beatrijs; De Smet, Olivia; Buysse, Ann; Steen, Johan; Vansteelandt, Stijn

    2013-11-01

    In the social sciences, mediation analysis has typically been formulated in the context of linear models using the Baron & Kenny (1986) approach. Extensions to nonlinear models have been considered but lack formal justification. By placing mediation analysis within the counterfactual framework of causal inference one can define causal mediation effects in a way that is not tied to a specific statistical model and identify them under certain no unmeasured confounding assumptions. Corresponding estimation procedures using parametric or nonparametric models, based on the so-called mediation formula, have recently been proposed in the psychological literature and made accessible through the R-package mediation. A number of limitations of the latter approach are discussed and a more flexible approach using natural effects models is proposed as an alternative. The latter builds on the same counterfactual framework but enables interpretable and parsimonious modeling of direct and mediated effects and facilitates tests of hypotheses that would otherwise be difficult or impossible to test. We illustrate the approach in a study of individuals who ended a romantic relationship and explore whether the effect of attachment anxiety during the relationship on unwanted pursuit behavior after the breakup is mediated by negative affect during the breakup.

  6. Auxin-dependent compositional change in Mediator in ARF7- and ARF19-mediated transcription.

    Science.gov (United States)

    Ito, Jun; Fukaki, Hidehiro; Onoda, Makoto; Li, Lin; Li, Chuanyou; Tasaka, Masao; Furutani, Masahiko

    2016-06-07

    Mediator is a multiprotein complex that integrates the signals from transcription factors binding to the promoter and transmits them to achieve gene transcription. The subunits of Mediator complex reside in four modules: the head, middle, tail, and dissociable CDK8 kinase module (CKM). The head, middle, and tail modules form the core Mediator complex, and the association of CKM can modify the function of Mediator in transcription. Here, we show genetic and biochemical evidence that CKM-associated Mediator transmits auxin-dependent transcriptional repression in lateral root (LR) formation. The AUXIN/INDOLE 3-ACETIC ACID 14 (Aux/IAA14) transcriptional repressor inhibits the transcriptional activity of its binding partners AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19 by making a complex with the CKM-associated Mediator. In addition, TOPLESS (TPL), a transcriptional corepressor, forms a bridge between IAA14 and the CKM component MED13 through the physical interaction. ChIP assays show that auxin induces the dissociation of MED13 but not the tail module component MED25 from the ARF7 binding region upstream of its target gene. These findings indicate that auxin-induced degradation of IAA14 changes the module composition of Mediator interacting with ARF7 and ARF19 in the upstream region of their target genes involved in LR formation. We suggest that this regulation leads to a quick switch of signal transmission from ARFs to target gene expression in response to auxin.

  7. Bioethics mediation: the role and importance of nursing advocacy.

    Science.gov (United States)

    Schlairet, Maura C

    2009-01-01

    Ethics consultations are utilized in health care to identify and manage conflict, difficult decision-making, and ethical issues. In bioethics mediation, a more updated approach using interpersonal, mediative, conflict management, and dispute resolution skills is merged with ethical principles to manage dilemmas arising in healthcare settings. This article argues, based on a professional obligation to advocate for the good of the client, that nurses must assume leadership roles in mediation processes. Nurses can initiate and fully participate in formal bioethics mediation and other mediative interventions. Nurse administrators can work to evolve existing ethics consult models to mediation models. Nonetheless, mediative efforts of individual nurses must be grounded in realization of the multifactorial nature of conflict and dilemma in healthcare settings. Multidisciplinary mediative interventions, framed by sound institutional policies, may best serve the complex needs of ethically vulnerable clients. To best advocate for these at-risk clients, nurses must assume various leadership roles in mediation processes.

  8. Proteinaceous molecules mediating Bifidobacterium-host interactions

    Directory of Open Access Journals (Sweden)

    Lorena Ruiz

    2016-08-01

    Full Text Available Bifidobacteria are commensal microoganisms found in the gastrointestinal tract.Several strains have been attributed beneficial traits at local and systemic levels, through pathogen exclusion or immune modulation, among other benefits. This has promoted a growing industrial and scientific interest in bifidobacteria as probiotic supplements. However, the molecular mechanisms mediating this cross-talk with the human host remain unknown. High-throughput technologies, from functional genomics to transcriptomics, proteomics and interactomics coupled to the development of both in vitro and in vivo models to study the dynamics of the intestinal microbiota and their effects on host cells, have eased the identification of key molecules in these interactions. Numerous secreted or surface-associated proteins or peptides have been identified as potential mediators of bifidobacteria-host interactions and molecular cross-talk, directly participating in sensing environmental factors, promoting intestinal colonization or mediating a dialogue with mucosa-associated immune cells. On the other hand, bifidobacteria induce the production of proteins in the intestine, by epithelial or immune cells, and other gut bacteria, which are key elements in orchestrating interactions among bifidobacteria, gut microbiota and host cells. This review aims to give a comprehensive overview on proteinaceous molecules described and characterized to date, as mediators of the dynamic interplay between bifidobacteria and the human host, providing a framework to identify knowledge gaps and future research needs.

  9. Collaborative learning through computer-mediated argumentation

    NARCIS (Netherlands)

    Kanselaar, G.; Veerman, A.L.; Andriessen, J.E.B.

    1999-01-01

    This article reports on three studies that involved undergraduate students collaboratively working on authentic discussion tasks in synchronous and asynchronous Computer Mediated Communication systems (Netmeeting, Belvédère, Allaire Forums). The purposes of the assignments were respectively to deve

  10. Mediating Artifact in Teacher Professional Development

    Science.gov (United States)

    Svendsen, Bodil

    2015-07-01

    This article focuses on teacher professional development (TPD) in natural science through the 5E model as mediating artifact. The study was conducted in an upper secondary school, grounded in a school-based intervention research project. My contribution to the field of research on TPD is founded on the hypothesis that teachers would be best facilitated to make their practice more inquiry based if they are provided with a mediating artifact. In this study the artifact is a model 5E, which is a conceptual way of thinking, to help teachers reflect on their practice. The aim is to encourage teachers to make changes themselves, by applying extended use of inquiry into their practice. This mediated artifact could thus be used across different national contexts. The main research question is; how can the 5E model as a mediating artifact enhance TPD? The article addresses the processes of the use of the 5E model and its influence on teachers' perception of the model. This is in order for teachers to conceptualize their goals related to inquiry and scientific thinking, and to solve the problems involved in achieving those goals in their own contexts. The study concludes that, after the intervention, the teachers' approaches and strategies demonstrate greater emphasis on learning.

  11. Immune-mediated canine and feline keratitis.

    Science.gov (United States)

    Andrew, Stacy E

    2008-03-01

    Although the normal cornea is devoid of vasculature and lymphatics, there are still several immune-mediated corneal conditions that can occur in dogs and cats. An overview of corneal immunology is presented. Diseases of dogs, including chronic superficial keratitis, superficial punctate keratitis, and canine adenovirus endotheliitis, as well as feline diseases, including eosinophilic keratitis and herpesvirus-related conditions, are discussed.

  12. RNAi-mediated plant protection against aphids.

    Science.gov (United States)

    Yu, Xiu-Dao; Liu, Zong-Cai; Huang, Si-Liang; Chen, Zhi-Qin; Sun, Yong-Wei; Duan, Peng-Fei; Ma, You-Zhi; Xia, Lan-Qin

    2016-06-01

    Aphids (Aphididae) are major agricultural pests that cause significant yield losses of crop plants each year by inflicting damage both through the direct effects of feeding and by vectoring harmful plant viruses. Expression of double-stranded RNA (dsRNA) directed against suitable insect target genes in transgenic plants has been shown to give protection against pests through plant-mediated RNA interference (RNAi). Thus, as a potential alternative and effective strategy for insect pest management in agricultural practice, plant-mediated RNAi for aphid control has received close attention in recent years. In this review, the mechanism of RNAi in insects and the so far explored effective RNAi target genes in aphids, their potential applications in the development of transgenic plants for aphid control and the major challenges in this regard are reviewed, and the future prospects of using plant-mediated RNAi for aphid control are discussed. This review is intended to be a helpful insight into the generation of aphid-resistant plants through plant-mediated RNAi strategy. © 2016 Society of Chemical Industry.

  13. Light Gaugino Problem in Direct Gauge Mediation

    CERN Document Server

    Ookouchi, Yutaka

    2011-01-01

    It has been known that in a wide class of direct gauge mediation models, the gaugino masses vanish at leading order in SUSY breaking. Recently, this phenomenon is understood in connection with the global structure of vacua in O'Raifeartaigh-type models. We review recent developments on this topic.

  14. A Mediated Lifespace: Working Relationally with Girls

    Science.gov (United States)

    Hoskins, Marie L.; Mathieson, Lindsay C.

    2004-01-01

    A lifespace has to do with how selves are mediated by combining multiple cultural symbols to construct an identity in the world. The authors cite examples from media texts, as well as from a narrative study conducted with 10 adolescent girls in a treatment program for eating disorders, that illustrate the importance of practitioners considering…

  15. Characterization of BASP1-mediated neurite outgrowth

    DEFF Research Database (Denmark)

    Korshunova, Irina; Caroni, Pico; Kolkova, Kateryna

    2008-01-01

    The brain acid-soluble protein BASP1 (CAP-23, NAP-22) belongs to the family of growth-associated proteins, which also includes GAP-43, a protein recently shown to regulate neural cell adhesion molecule (NCAM)-mediated neurite outgrowth. Here, the effects of BASP1 overexpression were investigated ...

  16. Web-Mediated Knowledge Synthesis for Educators

    Science.gov (United States)

    DeSchryver, Michael

    2015-01-01

    Ubiquitous and instant access to information on the Web is challenging what constitutes 21st century literacies. This article explores the notion of Web-mediated knowledge synthesis, an approach to integrating Web-based learning that may result in generative synthesis of ideas. This article describes the skills and strategies that may support…

  17. Plasmid mediated quinolone resistance in Enterobacteriaceae

    NARCIS (Netherlands)

    Veldman, K.T.; LS Klinisch Onderzoek Wagenaar

    2014-01-01

    This thesis describes the occurrence of Plasmid Mediated Quinolone Resistance (PMQR) in Salmonella and E. coli from The Netherlands and other European countries. Furthermore, the genetic background of these genes was characterized. Fluoroquinolones are widely used antibiotics in both human and veter

  18. Children and TV II: Mediating the Medium.

    Science.gov (United States)

    Winick, Mariann Pezzella; Wehrenberg, Judith S.

    This guidebook focuses on the reality of children's television viewing and the possibilities open to teachers for mediating the experience to enhance children's development and enrich the school curriculum. A Piagetian framework is used to examine and structure material suitable for the classroom. Part I of the guide reviews the historic…

  19. Improving Undergraduates' Critique via Computer Mediated Communication

    Science.gov (United States)

    Mohamad, Maslawati; Musa, Faridah; Amin, Maryam Mohamed; Mufti, Norlaila; Latiff, Rozmel Abdul; Sallihuddin, Nani Rahayu

    2014-01-01

    Our current university students, labeled as "Generation Y" or Millennials, are different from previous generations due to wide exposure to media. Being technologically savvy, they are accustomed to Internet for information and social media for socializing. In line with this current trend, teaching through computer mediated communication…

  20. Integrating Computer-Mediated Communication Strategy Instruction

    Science.gov (United States)

    McNeil, Levi

    2016-01-01

    Communication strategies (CSs) play important roles in resolving problematic second language interaction and facilitating language learning. While studies in face-to-face contexts demonstrate the benefits of communication strategy instruction (CSI), there have been few attempts to integrate computer-mediated communication and CSI. The study…

  1. Computer Mediated Communication (CMC e Second Life

    Directory of Open Access Journals (Sweden)

    Annalisa Boniello

    2013-03-01

    Full Text Available In questo contributo sono riportate alcune esperienze e sperimentazioni didattiche che descrivono occasioni di comunicazione formativa (Computer Mediated Communication – CMC attraverso ambienti 3D quali Second Life (SL. Sono inoltre evidenziati e descritti i principali strumenti di comunicazione in SL e il loro utilizzo.

  2. Synchronous Computer-Mediated Communication and Interaction

    Science.gov (United States)

    Ziegler, Nicole

    2016-01-01

    The current study reports on a meta-analysis of the relative effectiveness of interaction in synchronous computer-mediated communication (SCMC) and face-to-face (FTF) contexts. The primary studies included in the analysis were journal articles and dissertations completed between 1990 and 2012 (k = 14). Results demonstrate that interaction in SCMC…

  3. Conversation Analysis of Computer-Mediated Communication

    Science.gov (United States)

    Gonzalez-Lloret, Marta

    2011-01-01

    The potential of computer-mediated communication (CMC) for language learning resides mainly in the possibility that learners have to engage with other speakers of the language, including L1 speakers. The inclusion of CMC in the L2 classroom provides an opportunity for students to utilize authentic language in real interaction, rather than the more…

  4. Analyzing the mediated voice - a datasession

    DEFF Research Database (Denmark)

    Lawaetz, Anna

    Broadcasted voices are technologically manipulated. In order to achieve a certain autencity or sound of “reality” paradoxically the voices are filtered and trained in order to reach the listeners. This “mis-en-scene” is important knowledge when it comes to the development of a consistent method o...... of analysis of the mediated voice...

  5. Comments: Causal Interpretations of Mediation Effects

    Science.gov (United States)

    Jo, Booil; Stuart, Elizabeth A.

    2012-01-01

    The authors thank Dr. Lindsay Page for providing a nice illustration of the use of the principal stratification framework to define causal effects, and a Bayesian model for effect estimation. They hope that her well-written article will help expose education researchers to these concepts and methods, and move the field of mediation analysis in…

  6. A Comparison of Three Tests of Mediation

    Science.gov (United States)

    Warbasse, Rosalia E.

    2009-01-01

    A simulation study was conducted to evaluate the performance of three tests of mediation: the bias-corrected and accelerated bootstrap (Efron & Tibshirani, 1993), the asymmetric confidence limits test (MacKinnon, 2008), and a multiple regression approach described by Kenny, Kashy, and Bolger (1998). The evolution of these methods is reviewed and…

  7.  Fashion Mediators and Distributed Agency

    DEFF Research Database (Denmark)

    Sommerlund, Julie

    , and Belief - A New Sociology of Knowledge. J. Law. London, Routledge and Keagan Paul: 196-233. Hennion, A. (1997). "Baroque and rock: Music, mediators and musical taste." Poetics 24: 414 - 435. Latour, B. (1993). We Have Never Been Modern. New York, Harvester-Wheatsheaf....

  8. Computer-Mediated Communication: An Experimental Study.

    Science.gov (United States)

    Smith, William E.

    1994-01-01

    Investigates the effectiveness of a computer-mediated communication system in supplementing traditional instruction in a media law course. Finds mixed results on measures of satisfaction and no significant improvement on exam scores. Notes that the system required more time from the instructor and students. (SR)

  9. Impulsivity and Strategy Transfer: Metamemory as Mediator.

    Science.gov (United States)

    Borkowski, John G.; And Others

    1983-01-01

    Acquisition, maintenance, and generalization of organizational strategies were studied in two experiments as a function of impulsivity-reflectivity and metamemory among primary school students. Findings are in line with the hypothesis that metamemory, rather than cognitive tempo, mediates the effectiveness of an experimenter-trained strategy in…

  10. Teaching Responsibly with Technology-Mediated Communication

    Science.gov (United States)

    Veltsos, Jennifer R.; Veltsos, Christophe

    2010-01-01

    Technology-mediated communication, or "new media," such as blogs, Twitter, wikis, and social network sites, can be an endless source of ideas for activities or inspiration for classroom discussion. Many instructors ask students to monitor current events by following keywords and industry leaders on Twitter and reading both corporate and trade…

  11. Parental mediation of undesired advertising effects

    NARCIS (Netherlands)

    Buijzen, M.A.; Valkenburg, P.M.

    2005-01-01

    The aim of this study was to investigate the effectiveness of various types of parental mediation of 3 potentially undesired effects of television advertising. In a survey among 360 parent–child dyads with children in the 8 to 12 years age range, we investigated how different styles of advertising m

  12. Current correlators for general gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Ooguri, Hirosi [California Institute of Technology, 452-48 Pasadena, CA 91125 (United States); Institute for the Physics and Mathematics of the Universe, University of Tokyo, Kashiwa, Chiba 277-8582 (Japan); Ookouchi, Yutaka [California Institute of Technology, 452-48 Pasadena, CA 91125 (United States)], E-mail: yutaka@caltech.edu; Park, Chang-Soon; Song, Jaewon [California Institute of Technology, 452-48 Pasadena, CA 91125 (United States)

    2009-02-11

    In the gauge mediation mechanism, the effects of the hidden sector are characterized by a set of correlation functions of the global symmetry current of the hidden sector. We present methods to compute these correlators in cases with strongly coupled hidden sectors. Several examples are presented to demonstrate the technique explicitly.

  13. Excursion-Set-Mediated Genetic Algorithm

    Science.gov (United States)

    Noever, David; Baskaran, Subbiah

    1995-01-01

    Excursion-set-mediated genetic algorithm (ESMGA) is embodiment of method of searching for and optimizing computerized mathematical models. Incorporates powerful search and optimization techniques based on concepts analogous to natural selection and laws of genetics. In comparison with other genetic algorithms, this one achieves stronger condition for implicit parallelism. Includes three stages of operations in each cycle, analogous to biological generation.

  14. Teaching Responsibly with Technology-Mediated Communication

    Science.gov (United States)

    Veltsos, Jennifer R.; Veltsos, Christophe

    2010-01-01

    Technology-mediated communication, or "new media," such as blogs, Twitter, wikis, and social network sites, can be an endless source of ideas for activities or inspiration for classroom discussion. Many instructors ask students to monitor current events by following keywords and industry leaders on Twitter and reading both corporate and…

  15. Nutritionally Mediated Oxidative Stress and Inflammation

    Directory of Open Access Journals (Sweden)

    Alexandra Muñoz

    2013-01-01

    Full Text Available There are many sources of nutritionally mediated oxidative stress that trigger inflammatory cascades along short and long time frames. These events are primarily mediated via NFκB. On the short-term scale postprandial inflammation is characterized by an increase in circulating levels of IL-6 and TNF-α and is mirrored on the long-term by proinflammatory gene expression changes in the adipocytes and peripheral blood mononuclear cells (PBMCs of obese individuals. Specifically the upregulation of CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, CXCL2/MIP-2α, and CXCL3/MIP-2β is noted because these changes have been observed in both adipocytes and PBMC of obese humans. In comparing numerous human intervention studies it is clear that pro-inflammatory and anti-inflammatory consumption choices mediate gene expression in humans adipocytes and peripheral blood mononuclear cells. Arachidonic acid and saturated fatty acids (SFAs both demonstrate an ability to increase pro-inflammatory IL-8 along with numerous other inflammatory factors including IL-6, TNFα, IL-1β, and CXCL1 for arachidonic acid and IGB2 and CTSS for SFA. Antioxidant rich foods including olive oil, fruits, and vegetables all demonstrate an ability to lower levels of IL-6 in PBMCs. Thus, dietary choices play a complex role in the mediation of unavoidable oxidative stress and can serve to exacerbate or dampen the level of inflammation.

  16. 34 CFR 110.32 - Mediation.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Mediation. 110.32 Section 110.32 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION... the same time, and the meeting may be conducted by telephone or other means of effective dialogue if...

  17. Computer-Mediated Communication on the Internet.

    Science.gov (United States)

    Herring, Susan C.

    2002-01-01

    This review of computer-mediated communication (CMC) on the Internet focuses on empirical research in noninstitutional and nonorganizational contexts. Highlights include modes of CMC; appropriate uses; social effects; effects on language and communication; freedom of expression; community; personal impacts; privacy; ethics; democracy;…

  18. Cell-mediated mutagenesis by chemical carcinogens

    Energy Technology Data Exchange (ETDEWEB)

    Huberman, E.; Langenbach, R.

    1978-01-01

    The cell-mediated mutation system, with the proper choice of metabolizing cells, can be used to detect the mutagenic activities of different classes of chemical carcinogens. When fibroblastic cells were used as the metabolizing cells, a correlation between the in vivo carcinogenic activity and the in vitro mutagenic activity of 11 aromatic polycyclic hydrocarbons was observed. When primary liver cells were used as the metabolizing cells, three known liver carcinogens were demonstrated to be mutagenic by the cell-mediated assay, while two non-carcinogenic analogues were not mutagenic. These results from the cell-mediated system suggest that the reactive intermediates of the carcinogens are stable enough to be transferred from the metabolizing cells to the V79 cells. The cell-mediated mutagenesis system is a simple in vitro assay which may simulate the in vivo situation. It was concluded that this approach could be extended to the co-cultivation of cells from other organs or tissues with mutable mammalian cells.

  19. Deterministic mediated superdense coding with linear optics

    Energy Technology Data Exchange (ETDEWEB)

    Pavičić, Mladen, E-mail: mpavicic@physik.hu-berlin.de [Department of Physics—Nanooptics, Faculty of Mathematics and Natural Sciences, Humboldt University of Berlin (Germany); Center of Excellence for Advanced Materials and Sensing Devices (CEMS), Photonics and Quantum Optics Unit, Ruđer Bošković Institute, Zagreb (Croatia)

    2016-02-22

    We present a scheme of deterministic mediated superdense coding of entangled photon states employing only linear-optics elements. Ideally, we are able to deterministically transfer four messages by manipulating just one of the photons. Two degrees of freedom, polarization and spatial, are used. A new kind of source of heralded down-converted photon pairs conditioned on detection of another pair with an efficiency of 92% is proposed. Realistic probabilistic experimental verification of the scheme with such a source of preselected pairs is feasible with today's technology. We obtain the channel capacity of 1.78 bits for a full-fledged implementation. - Highlights: • Deterministic linear optics mediated superdense coding is proposed. • Two degrees of freedom, polarization and spatial, are used. • Heralded source of conditioned entangled photon pairs, 92% efficient, is proposed.

  20. Supersymmetry Breaking, Gauge Mediation, and the LHC

    Energy Technology Data Exchange (ETDEWEB)

    Shih, David [Rutgers Univ., New Brunswick, NJ (United States)

    2015-04-14

    Gauge mediated SUSY breaking (GMSB) is a promising class of supersymmetric models that automatically satisfies the precision constraints. Prior work of Meade, Seiberg and Shih in 2008 established the full, model-independent parameter space of GMSB, which they called "General Gauge Mediation" (GGM). During the first half of 2010-2015, Shih and his collaborators thoroughly explored the parameter space of GGM and established many well-motivated benchmark models for use by the experimentalists at the LHC. Through their work, the current constraints on GGM from LEP, the Tevatron and the LHC were fully elucidated, together with the possible collider signatures of GMSB at the LHC. This ensured that the full discovery potential for GGM could be completely realized at the LHC.

  1. Coagulation and Placenta-Mediated Complications

    Directory of Open Access Journals (Sweden)

    Anat Aharon

    2014-10-01

    Full Text Available Pregnancy is a physiological hypercoagulable state, preparing the mother for the hemostatic challenge of delivery. However, this is associated with an increased risk of venous thrombosis and placenta-mediated complications, which present major challenges for mother and fetus. Although these conditions are heterogeneous in their pathophysiology, hereditary and acquired thrombophilia has been associated with recurrent pregnancy loss and gestational vascular complications, such as early-onset pre-eclampsia and placental abruption. Prevention of such placenta-mediated complications, which collectively complicate up to 15% of pregnancies, is a major issue for women’s health. Prospective interventional studies stratified by current knowledge of pathophysiological mechanisms related to placental and systemic hemostatic alterations will impact on the management of pregnancies at risk of these complications.

  2. Components Interoperability through Mediating Connector Patterns

    CERN Document Server

    Spalazzese, Romina; 10.4204/EPTCS.37.3

    2010-01-01

    A key objective for ubiquitous environments is to enable system interoperability between system's components that are highly heterogeneous. In particular, the challenge is to embed in the system architecture the necessary support to cope with behavioral diversity in order to allow components to coordinate and communicate. The continuously evolving environment further asks for an automated and on-the-fly approach. In this paper we present the design building blocks for the dynamic and on-the-fly interoperability between heterogeneous components. Specifically, we describe an Architectural Pattern called Mediating Connector, that is the key enabler for communication. In addition, we present a set of Basic Mediator Patterns, that describe the basic mismatches which can occur when components try to interact, and their corresponding solutions.

  3. A Case Study of Mediated Urban Architecture

    DEFF Research Database (Denmark)

    Poulsen, Esben Skouboe; Andersen, Hans Jørgen; Jensen, Ole B.

    2012-01-01

    or proposing new interaction affordances. It is a design challenge to develop new tools and methods for analysis and design that utilizes new computational techniques as an integrated part of the mediated architectural concept - from the parametric sketch to CNC fabrication and into the illumination...... of the face expression of the building. We search for more informed, inspiring and appealing environments that challenge the role of the use of media in public spaces, and facilitate a new reconfigurable design trajectory that holds new social and spatial qualities in a highly mediated interactive urban...... and southeast, the pavilion became a shelter for the wind and established a more private space opening up towards the fiord and the sunset. Five seatings and a stage is furnishing the interior of the pavilion, while projectors, computers, loudspeakers and interior lightings represented the building’s media...

  4. Coping as a mediator of emotion.

    Science.gov (United States)

    Folkman, S; Lazarus, R S

    1988-03-01

    There is widespread conviction among health care professionals that coping affects emotion. Yet theory and research have traditionally emphasized the effects of emotion on coping. The present research addresses this imbalance by evaluating the extent to which coping mediated emotions during stressful encounters in two Caucasian, community-residing samples. Subjects' recently experienced stressful encounters, the ways they coped with the demands of those encounters, and the emotions they experienced during two stages of those encounters were assessed repeatedly. The extent to which eight forms of coping mediated each of four sets of emotions was evaluated with a series of hierarchical regression analyses (of residuals). Coping was associated with changes in all four sets of emotions, with some forms of coping associated with increases in positive emotions and other forms associated with increases in negative emotions.

  5. Seminatural Gauge Mediation from Product Group Unification

    CERN Document Server

    Fukuda, Hajime; Yanagida, Tsutomu T; Yokozaki, Norimi

    2015-01-01

    We propose a focus point gauge mediation model based on the product group unification (PGU), which solves the double-triplet splitting problem of the Higgs multiplets. In the focus point gauge mediation, the electroweak symmetry breaking scale can be naturally explained even for multi-TeV stops. It is known that the focus point behavior appears if a ratio of the number of SU(2) doublet messengers to that of SU(3) triplet messengers is close to 2.5. Importantly, this ratio (effectively) appears in our scenario based on the PGU, if the messenger field is an adjoint representation of SU(5) gauge group. Therefore, our focus point scenario is very predictive. It is also pointed out the gravitino can be dark matter without spoiling the success of the thermal leptogenesis.

  6. Cellular senescence mediates fibrotic pulmonary disease

    Science.gov (United States)

    Schafer, Marissa J.; White, Thomas A.; Iijima, Koji; Haak, Andrew J.; Ligresti, Giovanni; Atkinson, Elizabeth J.; Oberg, Ann L.; Birch, Jodie; Salmonowicz, Hanna; Zhu, Yi; Mazula, Daniel L.; Brooks, Robert W.; Fuhrmann-Stroissnigg, Heike; Pirtskhalava, Tamar; Prakash, Y. S.; Tchkonia, Tamara; Robbins, Paul D.; Aubry, Marie Christine; Passos, João F.; Kirkland, James L.; Tschumperlin, Daniel J.; Kita, Hirohito; LeBrasseur, Nathan K.

    2017-01-01

    Idiopathic pulmonary fibrosis (IPF) is a fatal disease characterized by interstitial remodelling, leading to compromised lung function. Cellular senescence markers are detectable within IPF lung tissue and senescent cell deletion rejuvenates pulmonary health in aged mice. Whether and how senescent cells regulate IPF or if their removal may be an efficacious intervention strategy is unknown. Here we demonstrate elevated abundance of senescence biomarkers in IPF lung, with p16 expression increasing with disease severity. We show that the secretome of senescent fibroblasts, which are selectively killed by a senolytic cocktail, dasatinib plus quercetin (DQ), is fibrogenic. Leveraging the bleomycin-injury IPF model, we demonstrate that early-intervention suicide-gene-mediated senescent cell ablation improves pulmonary function and physical health, although lung fibrosis is visibly unaltered. DQ treatment replicates benefits of transgenic clearance. Thus, our findings establish that fibrotic lung disease is mediated, in part, by senescent cells, which can be targeted to improve health and function. PMID:28230051

  7. Immune-Mediated Therapies for Liver Cancer

    Science.gov (United States)

    Aravalli, Rajagopal N.; Steer, Clifford J.

    2017-01-01

    In recent years, immunotherapy has gained renewed interest as an alternative therapeutic approach for solid tumors. Its premise is based on harnessing the power of the host immune system to destroy tumor cells. Development of immune-mediated therapies, such as vaccines, adoptive transfer of autologous immune cells, and stimulation of host immunity by targeting tumor-evasive mechanisms have advanced cancer immunotherapy. In addition, studies on innate immunity and mechanisms of immune evasion have enhanced our understanding on the immunology of liver cancer. Preclinical and clinical studies with immune-mediated therapies have shown potential benefits in patients with liver cancer. In this review, we summarize current knowledge and recent developments in tumor immunology by focusing on two main primary liver cancers: hepatocellular carcinoma and cholangiocarcinoma. PMID:28218682

  8. On messengers and metastability in gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Dudas, Emilian, E-mail: emilian.dudas@cpht.polytechnique.f [Centre de Physique Theorique, Ecole Polytechnique and CNRS, F-91128 Palaiseau (France); Laboratoire de Physique Theorique, Universite de Paris-Sud, Bat. 210, F-91405 Orsay (France); Lavignac, Stephane [Institut de Physique Theorique, CEA-Saclay, F-91191 Gif-sur-Yvette Cedex (France); Parmentier, Jeanne [Centre de Physique Theorique, Ecole Polytechnique and CNRS, F-91128 Palaiseau (France); Institut de Physique Theorique, CEA-Saclay, F-91191 Gif-sur-Yvette Cedex (France)

    2011-04-04

    One notoriously difficult problem in perturbative gauge mediation of supersymmetry breaking via messenger fields is the generic presence of a phenomenologically unacceptable vacuum with messenger vevs, with a lower energy than the desired ('MSSM') vacuum. We investigate the possibility that quantum corrections promote the latter to the ground state of the theory, and find that this is indeed feasible. For this to happen, the couplings of the messengers to the goldstino superfield must be small, and this implies an additional suppression of the MSSM soft terms with respect to the supersymmetry breaking scale. This in turn sets a lower limit on the masses of the messengers and of the supersymmetry breaking fields, which makes both sectors inaccessible at colliders. Contrary to other scenarios like direct gauge mediation, gaugino masses are unsuppressed with respect to scalar masses.

  9. Naturalness from Runaways in Direct Mediation

    Energy Technology Data Exchange (ETDEWEB)

    Schafer-Nameki, Sakura; /UC, Santa Barbara /King' s Coll. London; Tamarit, Carlos; /UC, Santa Barbara; Torroba, Gonzalo; /SLAC

    2011-02-07

    Postulating that the NMSSM singlet is a meson of a microscopic confining theory opens up new model-building possibilities. Based on this, we construct calculable models of direct mediation that solve the {mu}/B{mu} problem and simultaneously lead to realistic phenomenology. The singlet that couples to the Higgs fields develops a runaway produced by soft interactions, then stabilized by a small superpotential perturbation. The mechanism is first realized in an O'Raifeartaigh model of direct gauge mediation with metastable supersymmetry breaking. Focusing then on the microscopic theory, we argue that super QCD with massless and massive flavors in the free magnetic phase gives rise to this dynamics in the infrared. A deformation of the SQCD superpotential leads to large spontaneous R-symmetry breaking, gaugino masses naturally at the scale of the Higgs mass parameters, and absence of CP violating phases.

  10. Mediation Analyses in the Real World

    DEFF Research Database (Denmark)

    Lange, Theis; Starkopf, Liis

    2016-01-01

    The paper by Nguyen et al.1 published in this issue of Epidemiology presents a comparison of the recently suggested inverse odds ratio approach for addressing mediation and a more conventional Baron and Kenny-inspired method. Interestingly, the comparison is not done through a discussion of restr......The paper by Nguyen et al.1 published in this issue of Epidemiology presents a comparison of the recently suggested inverse odds ratio approach for addressing mediation and a more conventional Baron and Kenny-inspired method. Interestingly, the comparison is not done through a discussion...... more and more emphasis on ease of implementation, usability, and explanation; see, for instance, the SAS and SPSS macros by VanderWeele and Valeri 2 and the natural effects models implemented in the accompanying R package medflex by Vansteelandt and colleagues.3–5 Nguyen et al.1 also include R...

  11. Gaseous mediators in resolution of inflammation.

    Science.gov (United States)

    Wallace, John L; Ianaro, Angela; Flannigan, Kyle L; Cirino, Giuseppe

    2015-05-01

    There are numerous gaseous substances that can act as signaling molecules, but the best characterized of these are nitric oxide, hydrogen sulfide and carbon monoxide. Each has been shown to play important roles in many physiological and pathophysiological processes. This article is focused on the effects of these gasotransmitters in the context of inflammation. There is considerable overlap in the actions of nitric oxide, hydrogen sulfide and carbon monoxide with respect to inflammation, and these mediators appear to act primarily as anti-inflammatory substances, promoting resolution of inflammatory processes. They also have protective and pro-healing effects in some tissues, such as the gastrointestinal tract and lung. Over the past two decades, significant progress has been made in the development of novel anti-inflammatory and cytoprotective drugs that release of one or more of these gaseous mediators.

  12. 75 FR 52054 - Assessment of Mediation and Arbitration Procedures

    Science.gov (United States)

    2010-08-24

    ... Surface Transportation Board Assessment of Mediation and Arbitration Procedures AGENCY: Surface... matters relating to the use of mediation and arbitration as effective means of resolving disputes that are... mediation and arbitration in the resolution of disputes. If so, the proposed changes or new rules would...

  13. Organizational mediators siding with or against the powerful party?

    NARCIS (Netherlands)

    de Dreu, C.K.W.; Laskewitz, P.; van de Vliert, E.

    1994-01-01

    Examined whether, instead of being impartial, a mediator may side with one party as a function of the disputants' power differences (power balancing theory), the mediator's legitimacy judgments (siding theory), or the disputants' capacity to sanction the mediator (siding theory). A questionnaire stu

  14. 15 CFR 930.112 - Request for Secretarial mediation.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Request for Secretarial mediation. 930... RESOURCE MANAGEMENT FEDERAL CONSISTENCY WITH APPROVED COASTAL MANAGEMENT PROGRAMS Secretarial Mediation § 930.112 Request for Secretarial mediation. (a) The Secretary or other head of a Federal agency, or...

  15. 29 CFR 1202.2 - Interpretation of mediation agreements.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Interpretation of mediation agreements. 1202.2 Section 1202.2 Labor Regulations Relating to Labor (Continued) NATIONAL MEDIATION BOARD RULES OF PROCEDURE § 1202.2 Interpretation of mediation agreements. Under section 5, Second, of title I of the Railway...

  16. 29 CFR 1203.3 - Interpretation of mediation agreements.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Interpretation of mediation agreements. 1203.3 Section 1203.3 Labor Regulations Relating to Labor (Continued) NATIONAL MEDIATION BOARD APPLICATIONS FOR SERVICE § 1203.3 Interpretation of mediation agreements. (a) Applications may be filed with the Board's Chief...

  17. 7 CFR 900.103 - Application for mediation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Application for mediation. 900.103 Section 900.103 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... Application for mediation. An application for mediation by a cooperative, shall be in writing and...

  18. 45 CFR 400.83 - Mediation and fair hearings.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Mediation and fair hearings. 400.83 Section 400.83... Employment § 400.83 Mediation and fair hearings. (a) Mediation—(1) Public/private RCA program. The State must ensure that a mediation period prior to imposition of sanctions is provided to refugees by...

  19. 15 CFR 930.114 - Secretarial mediation efforts.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Secretarial mediation efforts. 930.114... MANAGEMENT FEDERAL CONSISTENCY WITH APPROVED COASTAL MANAGEMENT PROGRAMS Secretarial Mediation § 930.114 Secretarial mediation efforts. (a) Following the close of the hearing, the hearing officer shall transmit...

  20. 40 CFR 7.180 - Mediation of age discrimination complaints.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Mediation of age discrimination... Discrimination Prohibited on the Basis of Age § 7.180 Mediation of age discrimination complaints. (a) The OCR will refer all accepted complaints alleging age discrimination to the Mediation Agency designated...

  1. Mediation in civiele en bestuursrechtelijke zaken : Een internationaal literatuuronderzoek

    NARCIS (Netherlands)

    Baas, N.

    2002-01-01

    Doel van dit onderzoek is een overzicht krijgen van vormen van mediation van civiel- en bestuursrechtelijke zaken in binnen- en buitenland, de effectiviteit en efficientie van mediation-projecten en de factoren die van invloed zijn op de effectiviteit en efficiency van mediation.

  2. Mediation: Toward a Civilized System of Dispute Resolution.

    Science.gov (United States)

    Keltner, John W. Sam

    Because the intervention of a neutral third party is currently gaining favor as an alternative form of dispute resolution, this book explores the process of mediation in the context of managing struggle and examines some of the characteristics of mediators, their training and ethics, and the techniques and skills of good mediation. The theory…

  3. Prospective and Retrospective Processing in Associative Mediated Priming

    Science.gov (United States)

    Jones, Lara L.

    2012-01-01

    Mediated priming refers to the faster word recognition of a target (e.g., milk) following presentation of a prime (e.g., pasture) that is related indirectly via a connecting "mediator" (e.g., cow). Association strength may be an important factor in whether mediated priming occurs prospectively (with target activation prior to its presentation) or…

  4. Green oxidations with laccase-mediator systems.

    Science.gov (United States)

    Wells, A; Teria, M; Eve, T

    2006-04-01

    Laccases are oxidase enzymes produced by 'white rot' fungi as part of a complex armoury of redox enzymes used to break down lignin--part of the carbon cycle of nature. Laccases alone or in combination with redox co-catalysts have been shown to oxidize xenobiotic compounds under conditions that can be described as 'green'. This paper describes some novel oxidations using the laccase-mediator method and some current limitations to the use of this technology.

  5. Does Innovation Mediate Good Firm Performance?

    OpenAIRE

    Llanto, Gilberto M.; del Prado, Fatima

    2015-01-01

    Private firms invest in physical capital and human resource but they are also advised to invest in innovations to be more productive and profitable. Innovations refer to the development, deployment, and economic utilization of new products, processes, and services. It is important for firms to know whether investment in innovations is investment well-spent. Our empirical results provided an affirmative response to the question raised in this paper: "Does innovation mediate good firm performan...

  6. Immune-Mediated Therapies for Liver Cancer

    OpenAIRE

    Aravalli, Rajagopal N; Steer, Clifford J.

    2017-01-01

    In recent years, immunotherapy has gained renewed interest as an alternative therapeutic approach for solid tumors. Its premise is based on harnessing the power of the host immune system to destroy tumor cells. Development of immune-mediated therapies, such as vaccines, adoptive transfer of autologous immune cells, and stimulation of host immunity by targeting tumor-evasive mechanisms have advanced cancer immunotherapy. In addition, studies on innate immunity and mechanisms of immune evasion ...

  7. Mechanisms of platelet-mediated liver regeneration.

    Science.gov (United States)

    Lisman, Ton; Porte, Robert J

    2016-08-04

    Platelets have multiple functions beyond their roles in thrombosis and hemostasis. Platelets support liver regeneration, which is required after partial hepatectomy and acute or chronic liver injury. Although it is widely assumed that platelets stimulate liver regeneration by local excretion of mitogens stored within platelet granules, definitive evidence for this is lacking, and alternative mechanisms deserve consideration. In-depth knowledge of mechanisms of platelet-mediated liver regeneration may lead to new therapeutic strategies to treat patients with failing regenerative responses.

  8. Mediators of Oligodendrocyte Differentiation During Remyelination

    OpenAIRE

    Jigisha R Patel; Klein, Robyn S.

    2011-01-01

    Myelin, a dielectric sheath that wraps large axons in the central and peripheral nervous systems, is essential for proper conductance of axon potentials. In multiple sclerosis (MS), autoimmune-mediated damage to myelin within the central nervous system (CNS) leads to progressive disability primarily due to limited endogenous repair of demyelination with associated axonal pathology. While treatments are available to limit demyelination, no treatments are available to promote myelin repair. Stu...

  9. [Diagnosis of immune-mediated neuropathies].

    Science.gov (United States)

    Diószeghy, Péter

    2011-09-25

    Separate discussion of immune-mediated neuropathies from other neuropathies is justified by the serious consequences of the natural course of these diseases, like disability and sometimes even life threatening conditions. On the other hand nowadays effective treatments already exist, and with timely and correct diagnosis an appropriately chosen treatment may result in significant improvement of quality of life, occasionally even complete recovery. These are rare diseases, and the increasing number of different variants makes it more difficult to recognize them. Their diagnosis is based on the precise knowledge of clinical signs and symptoms, and it is verified by the help of neurophysiologic and laboratory, first of all CSF examinations. Description of clinical features of the classic acute immune-mediated neuropathy, characterized by ascending paresis and demyelination is followed by a summary of characteristics of newly recognized axonal, regional and functional variants. Chronic immune-mediated demyelinating polyneuropathies are not diagnosed in due number even today. This paper does not only present the classic form but it also introduces the ever increasing special variants, like distal acquired demyelinating sensory neuropathy, Lewis-Sumner syndrome, multifocal motor neuropathy and paraproteinemic neuropathies. Vasculitic neuropathies can be divided into two groups: systemic and non-systemic ones. The first sign of a vasculitic neuropathy is a progressive, painful mononeuropathy; the classic clinical presentation is the mononeuritis multiplex. It is characterized by general signs like fever, loss of weight, fatigue. In systemic vasculitis organ specific symptoms are also present. From the paraneoplastic diseases the subacute sensory neuropathy and the sensory neuronopathy are members of the immune-mediated neuropathies, being most frequently associated with small cell lung cancer.

  10. Ageing and cell-mediated immunity.

    Science.gov (United States)

    Fixa, B; Komárková, O; Chmelar, V

    1975-01-01

    The lymphocyte transformation test with phytohemagglutinin as mitogen estimated according to the incorporation of 2-(14)C-thymidine in DNA was used as an indicator of cell-mediated reactivity in 53 healthy subjects. Three age groups were examined: up to 20 years (21 subjects), 21-40 years (10 subjects) and over 70 years (22 subjects). The responsiveness of lymphocytes decreased significantly with age. In the highest age group 12 pathologically low values were found.

  11. ATP as a peripheral mediator of pain.

    Science.gov (United States)

    Hamilton, S G; McMahon, S B

    2000-07-01

    This article reviews the extent to which recent studies substantiate the hypothesis that ATP functions as a peripheral pain mediator. The discovery of the P2X family of ion channels (for which ATP is a ligand) and, in particular, the highly selective distribution of the P2X(3) receptor within the rat nociceptive system has inspired a variety of approaches to elucidate the potential role of ATP as a pain mediator. ATP elicits excitatory inward currents in small diameter sensory ganglion cells. These currents resemble those elicited by ATP on recombinantly expressed heteromeric P2X(2/3) channels as well as homomultimers consisting of P2X(2) and P2X(3). In vivo behavioural models have characterised the algogenic properties of ATP in normal conditions and in models of peripheral sensitisation. In humans, iontophoresis of ATP induces modest pain. In rats and humans the response is dependent on capsaicin sensitive neurons and is augmented in the presence of inflammatory mediators. Since ATP can be released in the vicinity of peripheral nociceptive terminals under a variety of conditions, there exists a purinergic chain of biological processes linking tissue damage to pain perception. The challenge remains to prove a physiological role for endogenous ATP in activating this chain of events.

  12. Lithium-mediated protection against ethanol neurotoxicity

    Directory of Open Access Journals (Sweden)

    Jia Luo

    2010-06-01

    Full Text Available Lithium has long been used as a mood stabilizer in the treatment of manic-depressive (bipolar disorder. Recent studies suggest that lithium has neuroprotective properties and may be useful in the treatment of acute brain injuries such as ischemia and chronic neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease and amyotrophic lateral sclerosis. One of the most important neuroprotective properties of lithium is its anti-apoptotic action. Ethanol is a neuroteratogen and fetal alcohol spectrum disorders (FASD are caused by maternal ethanol exposure during pregnancy. FASD is the leading cause of mental retardation. Ethanol exposure causes neuroapoptosis in the developing brain. Ethanol-induced loss of neurons in the central nervous system underlies many of the behavioral deficits observed in FASD. Excessive alcohol consumption is also associated with Wernicke–Korsakoff syndrome and neurodegeneration in the adult brain. Recent in vivo and in vitro studies indicate that lithium is able to ameliorate ethanol-induced neuroapoptosis. Lithium is an inhibitor of glycogen synthase kinase 3 (GSK3 which has recently been identified as a mediator of ethanol neurotoxicity. Lithium’s neuroprotection may be mediated by its inhibition of GSK3. In addition, lithium also affects many other signaling proteins and pathways that regulate neuronal survival and differentiation. This review discusses the recent evidence of lithium-mediated protection against ethanol neurotoxicity and potential underlying mechanisms.

  13. Endocytic pathways mediating oligomeric Aβ42 neurotoxicity

    Directory of Open Access Journals (Sweden)

    Laxton Kevin

    2010-05-01

    Full Text Available Abstract Background One pathological hallmark of Alzheimer's disease (AD is amyloid plaques, composed primarily of amyloid-β peptide (Aβ. Over-production or diminished clearance of the 42 amino acid form of Aβ (Aβ42 in the brain leads to accumulation of soluble Aβ and plaque formation. Soluble oligomeric Aβ (oAβ has recently emerged to be as a likely proximal cause of AD. Results Here we demonstrate that endocytosis is critical in mediating oAβ42-induced neurotoxicity and intraneuronal accumulation of Aβ. Inhibition of clathrin function either with a pharmacological inhibitor, knock-down of clathrin heavy chain expression, or expression of the dominant-negative mutant of clathrin-assembly protein AP180 did not block oAβ42-induced neurotoxicity or intraneuronal accumulation of Aβ. However, inhibition of dynamin and RhoA by expression of dominant negative mutants reduced neurotoxicity and intraneuronal Aβ accumulation. Pharmacologic inhibition of the dynamin-mediated endocytic pathway by genistein also reduced neurotoxicity. Conclusions These data suggest that dynamin-mediated and RhoA-regulated endocytosis are integral steps for oligomeric Aβ42-induced neurotoxicity and intraneuronal Aβ accumulation.

  14. Cryptographic Implications for Artificially Mediated Games

    CERN Document Server

    Meyer, Thomas Kellam

    2010-01-01

    There is currently an intersection in the research of game theory and cryptography. Generally speaking, there are two aspects to this partnership. First there is the application of game theory to cryptography. Yet, the purpose of this paper is to focus on the second aspect, the converse of the first, the application of cryptography to game theory. Chiefly, there exist a branch of non-cooperative games which have a correlated equilibrium as their solution. These equilibria tend to be superior to the conventional Nash equilibria. The primary condition for a correlated equilibrium is the presence of a mediator within the game. This is simply a neutral and mutually trusted entity. It is the role of the mediator to make recommendations in terms of strategy profiles to all players, who then act (supposedly) on this advice. Each party privately provides the mediator with the necessary information, and the referee responds privately with their optimized strategy set. However, there seem to be a multitude of situation...

  15. Bias in Cross-Sectional Analyses of Longitudinal Mediation: Partial and Complete Mediation under an Autoregressive Model

    Science.gov (United States)

    Maxwell, Scott E.; Cole, David A.; Mitchell, Melissa A.

    2011-01-01

    Maxwell and Cole (2007) showed that cross-sectional approaches to mediation typically generate substantially biased estimates of longitudinal parameters in the special case of complete mediation. However, their results did not apply to the more typical case of partial mediation. We extend their previous work by showing that substantial bias can…

  16. Plant Mediator complex and its critical functions in transcription regulation.

    Science.gov (United States)

    Yang, Yan; Li, Ling; Qu, Li-Jia

    2016-02-01

    The Mediator complex is an important component of the eukaryotic transcriptional machinery. As an essential link between transcription factors and RNA polymerase II, the Mediator complex transduces diverse signals to genes involved in different pathways. The plant Mediator complex was recently purified and comprises conserved and specific subunits. It functions in concert with transcription factors to modulate various responses. In this review, we summarize the recent advances in understanding the plant Mediator complex and its diverse roles in plant growth, development, defense, non-coding RNA production, response to abiotic stresses, flowering, genomic stability and metabolic homeostasis. In addition, the transcription factors interacting with the Mediator complex are also highlighted.

  17. Mediator and moderator effects in developmental and behavioral pediatric research.

    Science.gov (United States)

    Rose, Brigid M; Holmbeck, Grayson N; Coakley, Rachael Millstein; Franks, Elizabeth A

    2004-02-01

    The terms mediation and moderation are defined and clarified with particular emphasis on the role of mediational and moderational analyses in developmental and behavioral pediatric research. The article highlights the applicability of mediational and moderational analyses to longitudinal, intervention, and risk and protective factor research, and it provides basic information about how these analyses might be conducted. Also included is a discussion of various ways that both mediator and moderator variables can be incorporated into a single model. The article concludes with extended examples of both types of analyses using a longitudinal pediatric study for illustration. The article provides recommendations for applying mediational and moderational research in clinical practice.

  18. Neuroprotective strategies against calpain-mediated neurodegeneration

    Directory of Open Access Journals (Sweden)

    Yildiz-Unal A

    2015-02-01

    Full Text Available Aysegul Yildiz-Unal,1 Sirin Korulu,2 Arzu Karabay3 1Department of Molecular Biology and Genetics, Faculty of Science, Mugla Sitki Koçman University, Kötekli, Mugla, Turkey; 2Department of Molecular Biology and Genetics, Istanbul Arel University, Istanbul Turkey; 3Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Technical University, Maslak, Istanbul, Turkey Abstract: Calpains are calcium-dependent proteolytic enzymes that have deleterious effects on neurons upon their pathological over-activation. According to the results of numerous studies to date, there is no doubt that abnormal calpain activation triggers activation and progression of apoptotic processes in neurodegeneration, leading to neuronal death. Thus, it is very crucial to unravel all the aspects of calpain-mediated neurodegeneration in order to protect neurons through eliminating or at least minimizing its lethal effects. Protecting neurons against calpain-activated apoptosis basically requires developing effective, reliable, and most importantly, therapeutically applicable approaches to succeed. From this aspect, the most significant studies focusing on preventing calpain-mediated neurodegeneration include blocking the N-methyl-d-aspartate (NMDA-type glutamate receptor activities, which are closely related to calpain activation; directly inhibiting calpain itself via intrinsic or synthetic calpain inhibitors, or inhibiting its downstream processes; and utilizing the neuroprotectant steroid hormone estrogen and its receptors. In this review, the most remarkable neuroprotective strategies for calpain-mediated neurodegeneration are categorized and summarized with respect to their advantages and disadvantages over one another, in terms of their efficiency and applicability as a therapeutic regimen in the treatment of neurodegenerative diseases. Keywords: calpain, neurodegeneration, neuroprotection, calpain inhibitors, NMDAR, Speedy/RINGO

  19. Inflammatory mediators in mastitis and lactation insufficiency.

    Science.gov (United States)

    Ingman, Wendy V; Glynn, Danielle J; Hutchinson, Mark R

    2014-07-01

    Mastitis is a common inflammatory disease during lactation that causes reduced milk supply. A growing body of evidence challenges the central role of pathogenic bacteria in mastitis, with disease severity associated with markers of inflammation rather than infection. Inflammation in the mammary gland may be triggered by microbe-associated molecular patterns (MAMPs) as well as danger-associated molecular patterns (DAMPs) binding to pattern recognition receptors such as the toll-like receptors (TLRs) on the surface of mammary epithelial cells and local immune cell populations. Activation of the TLR4 signalling pathway and downstream nuclear factor kappa B (NFkB) is critical to mediating local mammary gland inflammation and systemic immune responses in mouse models of mastitis. However, activation of NFkB also induces epithelial cell apoptosis and reduced milk protein synthesis, suggesting that inflammatory mediators activated during mastitis promote partial involution. Perturbed milk flow, maternal stress and genetic predisposition are significant risk factors for mastitis, and could lead to a heightened TLR4-mediated inflammatory response, resulting in increased susceptibility and severity of mastitis disease in the context of low MAMP abundance. Therefore, heightened host inflammatory signalling may act in concert with pathogenic or commensal bacterial species to cause both the inflammation associated with mastitis and lactation insufficiency. Here, we present an alternate paradigm to the widely held notion that breast inflammation is driven principally by infectious bacterial pathogens, and suggest there may be other therapeutic strategies, apart from the currently utilised antimicrobial agents, that could be employed to prevent and treat mastitis in women.

  20. Mediation analysis allowing for exposure-mediator interactions and causal interpretation: theoretical assumptions and implementation with SAS and SPSS macros.

    Science.gov (United States)

    Valeri, Linda; Vanderweele, Tyler J

    2013-06-01

    Mediation analysis is a useful and widely employed approach to studies in the field of psychology and in the social and biomedical sciences. The contributions of this article are several-fold. First we seek to bring the developments in mediation analysis for nonlinear models within the counterfactual framework to the psychology audience in an accessible format and compare the sorts of inferences about mediation that are possible in the presence of exposure-mediator interaction when using a counterfactual versus the standard statistical approach. Second, the work by VanderWeele and Vansteelandt (2009, 2010) is extended here to allow for dichotomous mediators and count outcomes. Third, we provide SAS and SPSS macros to implement all of these mediation analysis techniques automatically, and we compare the types of inferences about mediation that are allowed by a variety of software macros.

  1. Urticaria and Allergy-Mediated Conditions.

    Science.gov (United States)

    Jafilan, Lena; James, Charis

    2015-12-01

    Urticaria is a common condition that involves pruritic, raised skin wheals. Although urticaria is a benign, self-limiting condition, it may cause frustration for patients, often because of its chronicity and its tendency to recur. It can also be a life-threatening allergic reaction. Diagnosis is made clinically. It affects 20% of the general population. The first-line treatment for nonremitting cases includes H-1anti-histamines. However, other therapies may be employed. Other allergy-mediated skin conditions include angioedema, contact dermatitis, and atopic dermatitis. Diagnosis is clinical, and management focuses on prevention, avoiding triggers, and treating the itching and inflammation that accompany these conditions.

  2. Activism and the Online Mediation Opportunity Structure

    DEFF Research Database (Denmark)

    Uldam, Julie

    2013-01-01

    The annual United Nations (UN) Framework Convention on Climate Change conferences provides a transnational mediation opportunity structure for activist networks to contest policies that favor market-based models for solving the climate crisis. Online technologies, including commercial social media...... to climate change activism. This impedes possibilities for using online media to protest at the radical end of the climate justice movement spectrum. This article explores this interrelationship between activist demands and (online) modes of action through a focus on the mobilization efforts of London...

  3. Gauge mediated supersymmetry breaking and neutrino anomalies

    CERN Document Server

    Joshipura, A S; Joshipura, Anjan S.; Vempati, Sudhir K.

    1999-01-01

    Supersymmetric standard model with softly broken lepton symmetry provides a suitable framework to accommodate the solar and atmospheric neutrino anomalies. This model contains a natural explanation for large mixing and hierarchal masses without fine tuning of the parameters. Neutrino spectrum is particularly constrained in the minimal messenger model (MMM) of gauge mediated SUSY breaking, since all SUSY breaking effects are controlled in MMM by a single parameter. We study the structure of neutrino masses and mixing both in MMM and in simple extensions of it in the context of solar and atmospheric neutrino anomalies.

  4. SV40-mediated immortalization of human fibroblasts.

    Science.gov (United States)

    Ozer, H L; Banga, S S; Dasgupta, T; Houghton, J; Hubbard, K; Jha, K K; Kim, S H; Lenahan, M; Pang, Z; Pardinas, J R; Patsalis, P C

    1996-01-01

    We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p53 via complex formation with T antigen. However, immortalization additionally requires inactivation of a novel growth suppressor gene, which has recently been identified to be on the distal portion of the long arm of chromosome 6, designated SEN6. We propose that SEN6 is responsible for cellular senescence in fibroblasts and other cells.

  5. The Forgotten Peace : Mediation at Niagara Falls

    OpenAIRE

    Small, Michael

    2010-01-01

    In the early hours of April 22, 1914, American President Woodrow Wilson sent Marines to seize the port of Veracruz in an attempt to alter the course of the Mexican Revolution. As a result, the United States seemed on the brink of war with Mexico. An international uproar ensued. The governments of Argentina, Brazil, and Chile offered to mediate a peaceful resolution to the crisis. Surprisingly, both the United States and Mexico accepted their offer and all parties agreed to meet at an internat...

  6. Mediated Trust in the Innovation Ecosystem

    DEFF Research Database (Denmark)

    Mogensen, Kirsten

    This paper introduces a framework for further studies in the role of mediated trust in the innovation ecosystems. Combining insight from scholars like Etzkowitz, Russel and Nordfors, the concept of innovation ecosystem is described as an extended Triple Helix where media is seen as a fourth strand...... should be considered trustworthy. When receiving the message, the audience will decode the signals. Trust is considered fundamental for mass media if these are to fulfill their normative functions in the innovation ecosystem. However, the concept of trust is not clearly defined in the literature and we...

  7. Mediation of supersymmetry breaking in extra dimensions

    CERN Document Server

    Scrucca, C A

    2004-01-01

    I review the mechanisms of supersymmetry breaking mediation that occur in sequestered models, where the visible and the hidden sectors are separated by an extra dimension and communicate only via gravitational interactions. By locality, soft breaking terms are forbidden at the classical level and reliably computable within an effective field theory approach at the quantum level. I present a self-contained discussion of these radiative gravitational effects and the resulting pattern of soft masses, and give an overview of realistic model building based on this set-up. I consider both flat and warped extra dimensions, as well as the possibility that there be localized kinetic terms for the gravitational fields.

  8. Mediating multimodal environmental knowledge across animation techniques

    DEFF Research Database (Denmark)

    Maier, Carmen Daniela

    2011-01-01

    in the technology- mediated discourse of short animation films. This is done by employing the model of multimodal discourse analysis proposed by Van Leeuwen (2008) and Machin and Van Leeuwen (2007) to the analysis of a series of animation films focused on environmental problems. These films are united under......://www.sustainlane.com/. The multimodal discourse analysis is meant to reveal how selection and representation of environmental knowledge about social actors, social actions, resources, time and space are influenced by animation techniques. Furthermore, in the context of this multimodal discourse analysis, their influence upon...

  9. Soluble Mediators Regulating Immunity in Early Life

    Directory of Open Access Journals (Sweden)

    Matthew Aaron Pettengill

    2014-09-01

    Full Text Available Soluble factors in blood plasma have a substantial impact on both the innate and adaptive immune responses. The complement system, antibodies, and antimicrobial proteins and peptides (APPs, can directly interact with potential pathogens, protecting against systemic infection. The extracellular environment also has a critical influence on immune cell maturation, activation, and effector functions, and many of the factors in plasma, including hormones, vitamins, and purines, have been shown to influence these processes for leukocytes of both the innate and adaptive immune systems. In this review we give particular consideration to soluble mediators in plasma for which age-dependent differences in abundance may influence the ontogeny of immune function.

  10. Gravitino dark matter in gravity mediation

    Energy Technology Data Exchange (ETDEWEB)

    Kersten, Joern [Hamburg Univ. (Germany). II. Inst. fuer Theoretische Physik; Lebedev, Oleg [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)

    2009-06-15

    We study general conditions for the gravitino to be the lightest supersymmetric particle (LSP) in models with gravity mediated supersymmetry breaking. We find that the decisive quantities are the Kaehler potential K and the gauge kinetic function f. In constrained MSSM (CMSSM) type models, the gravitino LSP occurs if the gaugino mass at the GUT scale is greater than approximately 2.5 gravitino masses. This translates into {radical}(K'')/f'<0.2, where the derivatives are taken with respect to the dominant SUSY breaking field. This requirement can easily be satisfied in string-motivated setups. (orig.)

  11. Dirac gauginos in general gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Benakli, K. [Laboratoire de Physique Theorique et Hautes Energies, CNRS, UPMC Univ Paris 06, Boite 126, 4 place Jussieu, F-75252 Paris Cedex 05 (France)], E-mail: kbenakli@lpthe.jussieu.fr; Goodsell, M.D. [Laboratoire de Physique Theorique et Hautes Energies, CNRS, UPMC Univ Paris 06, Boite 126, 4 place Jussieu, F-75252 Paris Cedex 05 (France)], E-mail: goodsell@lpthe.jussieu.fr

    2009-07-21

    We extend the formulation by Meade, Seiberg and Shih of general gauge mediation of supersymmetry breaking to include Dirac masses for the gauginos. These appear through mixing of the visible sector gauginos with additional states in adjoint representations. We illustrate the method by reproducing the existing results in the literature for the gaugino and sfermion masses when preserving R-symmetry. We then explain how the generation of same sign masses for the two propagating degrees of freedom in the adjoint scalars can be achieved. We end by commenting on the use of the formalism for describing U(1) mixing.

  12. Gravitino dark matter in gravity mediation

    Energy Technology Data Exchange (ETDEWEB)

    Kersten, Joern [University of Hamburg, II. Institute for Theoretical Physics, Luruper Chaussee 149, 22761 Hamburg (Germany); Lebedev, Oleg [DESY Theory Group, Notkestrasse 85, 22603 Hamburg (Germany)], E-mail: lebedev@mail.desy.de

    2009-08-03

    We study general conditions for the gravitino to be the lightest supersymmetric particle (LSP) in models with gravity mediated supersymmetry breaking. We find that the decisive quantities are the Kaehler potential K and the gauge kinetic function f. In constrained MSSM (CMSSM) type models, the gravitino LSP occurs if the gaugino mass at the GUT scale is greater than approximately 2.5 gravitino masses. This translates into {radical}(K{sup ''})/f{sup '}<0.2, where the derivatives are taken with respect to the dominant SUSY breaking field. This requirement can easily be satisfied in string-motivated setups.

  13. SUSY CP problem in gauge mediation model

    Energy Technology Data Exchange (ETDEWEB)

    Moroi, Takeo [Department of Physics, University of Tokyo, Tokyo 113-0033 (Japan); Yokozaki, Norimi, E-mail: yokozaki@hep-th.phys.s.u-tokyo.ac.jp [Department of Physics, University of Tokyo, Tokyo 113-0033 (Japan)

    2011-07-27

    SUSY CP problem in the gauge mediation supersymmetry breaking model is reconsidered. We pay particular attention to two sources of CP violating phases whose effects were not seriously studied before; one is the effect of the breaking of the GUT relation among the gaugino masses due to the field responsible for the GUT symmetry breaking, and the other is the supergravity effect on the supersymmetry breaking parameters, in particular, on the bi-linear supersymmetry breaking Higgs mass term. We show that both of them can induce too large electric dipole moments of electron, neutron, and so on, to be consistent with the experimental bounds.

  14. Focus point from direct gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Sibo [Chongqing University, Department of Physics, Chongqing (China)

    2015-01-01

    This paper is devoted to the reconciliation of the tension between the theoretic expectation from naturalness and the present LHC limits on the superpartner mass bounds. We argue that in supersymmetry models of direct gauge mediation the focusing phenomenon appears, which dramatically reduces the fine tuning associated to the 126 GeV Higgs boson. This type of model is highly predictive as regards the mass spectrum, with a multi-TeV third generation, the A{sub t} term of order 1 TeV, gluino mass above LHC mass bound, and the light neutralinos and charginos below 1 TeV. (orig.)

  15. Meganuclease and transposon mediated transgenesis in medaka.

    Science.gov (United States)

    Grabher, Clemens; Wittbrodt, Joachim

    2007-01-01

    From among a plethora of various gene delivery methods, the researcher must choose the right one according to availability for a given species and the precise application the transgenic animal is intended for. Here we review the progress in meganuclease and Sleeping Beauty transposon mediated transgenesis over recent years with a focus on medaka and zebrafish. We present a side-by-side comparison of these two approaches based on their biologic properties and provide interesting perspectives for future experiments and applications, which are different for the two techniques because of their distinct modes of action.

  16. Agrobacterium-mediated transformation: rice transformation.

    Science.gov (United States)

    Slamet-Loedin, Inez H; Chadha-Mohanty, Prabhjit; Torrizo, Lina

    2014-01-01

    Agrobacterium is a common soil bacterium with natural capacity for trans-kingdom transfer of genetic information by transferring its T-DNA into the eukaryotic genome. In agricultural plant biotechnology, combination of non-phytopathogenic strain of Agrobacterium tumefaciens with modified T-DNA and vir-genes in a binary vector system is the most widely utilized system for genetic improvement in diverse plant species and for gene function validation. Here we have described a highly efficient A. tumefaciens-mediated transformation system for indica and japonica rice cultivars based on an immature embryo system.

  17. HEMOLYTIC ANEMIA IMUNNE-MEDIATED IN DOGS

    Directory of Open Access Journals (Sweden)

    R. C. Castilho

    2016-11-01

    Full Text Available Due to the reduction in the number of red blood cells, caused by the immune system, the immune-mediated hemolytic anemia (IMHA is the most common disease among the hemolytic anemias and occurs more frequently in dogs (Nelson & Couto, 2010, wherein the most affected breeds are Cocker Spaniel, Poodle, Doberman and Collie (ETTINGER; FELDMAN 2004; THRALL et al 2007.. There is no pathognomonic sign for the diagnosis of the immune-mediated hemolytic anemia; however, laboratory findings show regenerative anemia, spherocytosis, positive results in Coombs' test and rarely, monocytes with hemosiderin or erythrocytes phagocytosis, but even with these findings, the primary and secondary IMHA can not be differentiate from each other. Differentiation can only be achieved when there is a deep investigation into the cause of the anemia. The IMHA therapeutics starts with the support treatment and follows with an immunosuppressive therapy. In relation to IMHA Mortality rates, the numbers range from 25% to 50% (Thrall, 2007, or above 70% (CARR; Panciera; Kidd, 2002.

  18. Elastase mediated fibrinolysis in acute promyelocytic leukemia.

    Science.gov (United States)

    Oudijk, E J; Nieuwenhuis, H K; Bos, R; Fijnheer, R

    2000-06-01

    The bleeding syndrome of acute promyelocytic leukemia (APL) is complex and consists of disseminated intravascular coagulation (DIC) and hyperfibrinolysis. Elastase, derived from malignant promyelocytes, is believed to mediate the fibrinogeno- and fibrinolysis by aspecific proteolysis. In this study we measured the role of elastase in fifteen patients with APL by using an assay for elastase degraded fibrin(ogen) and the results were compared with those obtained in patients with sepsis induced DIC. High levels of elastase were observed in sepsis and APL. The levels of fibrinogen and fibrin degradation products were significantly higher in APL patients compared to patients with sepsis induced DIC. Nevertheless, the level of elastase degraded fibrin(ogen) was higher in the sepsis group (635.3 ng/ml, compared to 144.3 ng/ml in APL; p <0.0001). So, the enormous increase in fibrin and fibrinogen degradation products in APL cannot be explained by elastase activity. This study suggests a minor role for elastase mediated proteolysis in the hemorrhagic diathesis in APL patients.

  19. FRNK negatively regulates IL-4-mediated inflammation.

    Science.gov (United States)

    Sharma, Ritu; Colarusso, Pina; Zhang, Hong; Stevens, Katarzyna M; Patel, Kamala D

    2015-02-15

    Focal adhesion kinase (FAK)-related nonkinase (PTK2 isoform 6 in humans, hereafter referred to as FRNK) is a cytoskeletal regulatory protein that has recently been shown to dampen lung fibrosis, yet its role in inflammation is unknown. Here, we show for the first time that expression of FRNK negatively regulates IL-4-mediated inflammation in a human model of eosinophil recruitment. Mechanistically, FRNK blocks eosinophil accumulation, firm adhesion and transmigration by preventing transcription and protein expression of VCAM-1 and CCL26. IL-4 activates STAT6 to induce VCAM-1 and CCL26 transcription. We now show that IL-4 also increases GATA6 to induce VCAM-1 expression. FRNK blocks IL-4-induced GATA6 transcription but has little effect on GATA6 protein expression and no effect on STAT6 activation. FRNK can block FAK or Pyk2 signaling and we, thus, downregulated these proteins using siRNA to determine whether signaling from either protein is involved in the regulation of VCAM-1 and CCL26. Knockdown of FAK, Pyk2 or both had no effect on VCAM-1 or CCL26 expression, which suggests that FRNK acts independently of FAK and Pyk2 signaling. Finally, we found that IL-4 induces the late expression of endogenous FRNK. In summary, FRNK represents a novel mechanism to negatively regulate IL-4-mediated inflammation.

  20. Mediators between bereavement and somatic symptoms

    Directory of Open Access Journals (Sweden)

    Konkolÿ Thege Barna

    2012-06-01

    Full Text Available Abstract Background In our research we examined the frequency of somatic symptoms among bereaved (N = 185 and non-bereaved men and women in a national representative sample (N = 4041 and investigated the possible mediating factors between bereavement status and somatic symptoms. Methods Somatic symptoms were measured by the Patient Health Questionnaire (PHQ-15, anxiety with a four-point anxiety rating scale, and depression with a nine-item shortened version of the Beck Depression Inventory. Results Among the bereaved, somatic symptoms proved to be significantly more frequent in both genders when compared to the non-bereaved, as did anxiety and depression. On the multivariate level, the results show that both anxiety and depression proved to be a mediator between somatic symptoms and bereavement. The effect sizes indicated that for both genders, anxiety was a stronger predictor of somatic symptoms than depression. Conclusions The results of our research indicate that somatic symptoms accompanying bereavement are not direct consequences of this state but they can be traced back to the associated anxiety and depression. These results draw attention to the need to recognize anxiety and depression looming in the background of somatic complaints in bereavement and to the importance of the dissemination of related information.

  1. Hydrogen Sulfide and Polysulfides as Biological Mediators

    Directory of Open Access Journals (Sweden)

    Hideo Kimura

    2014-10-01

    Full Text Available Hydrogen sulfide (H2S is recognized as a biological mediator with various roles such as neuromodulation, regulation of the vascular tone, cytoprotection, anti-inflammation, oxygen sensing, angiogenesis, and generation of mitochondrial energy. It is produced by cystathionine β-synthase (CBS, cystathionine γ-lyase (CSE, and 3-mercaptopyruvate sulfurtransferase (3MST. The activity of CBS is enhanced by S-adenosyl methionine (SAM and glutathionylation, while it is inhibited by nitric oxide (NO and carbon monoxide (CO. The activity of CSE and cysteine aminotransferase (CAT, which produces the 3MST substrate 3-mercaptopyruvate (3MP, is regulated by Ca2+. H2S is oxidized to thiosulfate in mitochondria through the sequential action of sulfide quinone oxidoreductase (SQR, sulfur dioxygenase, and rhodanese. The rates of the production and clearance of H2S determine its cellular concentration. Polysulfides (H2Sn have been found to occur in the brain and activate transient receptor potential ankyrin 1 (TRPA1 channels, facilitate the translocation of nuclear factor erythroid 2-related factor 2 (Nrf2 to the nucleus, and suppress the activity of phosphatase and tensin homolog (PTEN by sulfurating (sulfhydrating the target cysteine residues. A cross talk between H2S and NO also plays an important role in cardioprotection as well as regulation of the vascular tone. H2S, polysulfides, and their cross talk with NO may mediate various physiological and pathophysiological responses.

  2. Metallothionein as an Anti-Inflammatory Mediator

    Directory of Open Access Journals (Sweden)

    Ken-ichiro Inoue

    2009-01-01

    Full Text Available The integration of knowledge concerning the regulation of MT, a highly conserved, low molecular weight, cystein-rich metalloprotein, on its proposed functions is necessary to clarify how MT affects cellular processes. MT expression is induced/enhanced in various tissues by a number of physiological mediators. The cellular accumulation of MT depends on the availability of cellular zinc derived from the diet. MT modulates the binding and exchange/transport of heavy metals such as zinc, cadmium, or copper under physiological conditions and cytoprotection from their toxicities, and the release of gaseous mediators such as hydroxyl radicals or nitric oxide. In addition, MT reportedly affects a number of cellular processes, such as gene expression, apoptosis, proliferation, and differentiation. Given the genetic approach, the apparently healthy status of MT-deficient mice argues against an essential biological role for MT; however, this molecule may be critical in cells/tissues/organs in times of stress, since MT expression is also evoked/enhanced by various stresses. In particular, because metallothionein (MT is induced by inflammatory stress, its roles in inflammation are implied. Also, MT expression in various organs/tissues can be enhanced by inflammatory stimuli, implicating in inflammatory diseases. In this paper, we review the role of MT of various inflammatory conditions.

  3. Ultrasound-mediated nail drug delivery system.

    Science.gov (United States)

    Abadi, Danielle; Zderic, Vesna

    2011-12-01

    A novel ultrasound-mediated drug delivery system has been developed for treatment of a nail fungal disorder (onychomycosis) by improving delivery to the nail bed using ultrasound to increase the permeability of the nail. The slip-in device consists of ultrasound transducers and drug delivery compartments above each toenail. The device is connected to a computer, where a software interface allows users to select their preferred course of treatment. In in vitro testing, canine nails were exposed to 3 energy levels (acoustic power of 1.2 W and exposure durations of 30, 60, and 120 seconds). A stereo -microscope was used to determine how much of a drug-mimicking compound was delivered through the nail layers by measuring brightness on the cross section of each nail tested at each condition, where brightness level decreases coincide with increases in permeability. Each of the 3 energy levels tested showed statistical significance when compared to the control (P permeability factor of 1.3 after 30 seconds of exposure, 1.3 after 60 seconds, and 1.5 after 120 seconds, where a permeability factor of 1 shows no increase in permeability. Current treatments for onychomycosis include systemic, topical, and surgical. Even when used all together, these treatments typically take a long time to result in nail healing, thus making this ultrasound-mediated device a promising alternative.

  4. Histone chaperone-mediated nucleosome assembly process.

    Science.gov (United States)

    Fan, Hsiu-Fang; Liu, Zi-Ning; Chow, Sih-Yao; Lu, Yi-Han; Li, Hsin

    2015-01-01

    A huge amount of information is stored in genomic DNA and this stored information resides inside the nucleus with the aid of chromosomal condensation factors. It has been reported that the repeat nucleosome core particle (NCP) consists of 147-bp of DNA and two copies of H2A, H2B, H3 and H4. Regulation of chromosomal structure is important to many processes inside the cell. In vivo, a group of histone chaperones facilitate and regulate nucleosome assembly. How NCPs are constructed with the aid of histone chaperones remains unclear. In this study, the histone chaperone-mediated nucleosome assembly process was investigated using single-molecule tethered particle motion (TPM) experiments. It was found that Asf1 is able to exert more influence than Nap1 and poly glutamate acid (PGA) on the nucleosome formation process, which highlights Asf1's specific role in tetrasome formation. Thermodynamic parameters supported a model whereby energetically favored nucleosomal complexes compete with non-nucleosomal complexes. In addition, our kinetic findings propose the model that histone chaperones mediate nucleosome assembly along a path that leads to enthalpy-favored products with free histones as reaction substrates.

  5. Cellular contractility requires ubiquitin mediated proteolysis.

    Directory of Open Access Journals (Sweden)

    Yuval Cinnamon

    Full Text Available BACKGROUND: Cellular contractility, essential for cell movement and proliferation, is regulated by microtubules, RhoA and actomyosin. The RhoA dependent kinase ROCK ensures the phosphorylation of the regulatory Myosin II Light Chain (MLC Ser19, thereby activating actomyosin contractions. Microtubules are upstream inhibitors of contractility and their depolymerization or depletion cause cells to contract by activating RhoA. How microtubule dynamics regulates RhoA remains, a major missing link in understanding contractility. PRINCIPAL FINDINGS: We observed that contractility is inhibited by microtubules not only, as previously reported, in adherent cells, but also in non-adhering interphase and mitotic cells. Strikingly we observed that contractility requires ubiquitin mediated proteolysis by a Cullin-RING ubiquitin ligase. Inhibition of proteolysis, ubiquitination and neddylation all led to complete cessation of contractility and considerably reduced MLC Ser19 phosphorylation. CONCLUSIONS: Our results imply that cells express a contractility inhibitor that is degraded by ubiquitin mediated proteolysis, either constitutively or in response to microtubule depolymerization. This degradation seems to depend on a Cullin-RING ubiquitin ligase and is required for cellular contractions.

  6. The Journey Metaphor in Mediatized Political Discourse

    Directory of Open Access Journals (Sweden)

    Dávid Gyula

    2015-12-01

    Full Text Available The present paper will analyse manifestations of the journey metaphor from a critical discourse analytical perspective in order to observe how the journey metaphor is used as a discourse strategy in mediatized political speeches and interviews whereby political actors manipulate the second-frame interactional participants (the audience into sharing a (spurious sense of solidarity with them. There are three hypotheses that will be tested in the course of the analysis: the first is that a wide-variety of realjourney elements are exploited for the political metaphor of journey, and there is a concrete correspondence between journey vehicles and political scenarios. The second hypothesis is that journey metaphors that are used in political speeches, celebrity interviews and confrontational political interviews are of different types and complexity. The third hypothesis is that the manipulative intent behind the use of metaphors is exposed in the latter types of mediatized political discourse to varying degrees as a result of the different degrees of pragmatic accountability adhered to in the two subgenres. We argue that the first two hypotheses are confirmed on the basis of the qualitative analysis presented in the paper, whereas the third hypothesis is not borne out by the data.

  7. Hydrogen sulfide and polysulfides as biological mediators.

    Science.gov (United States)

    Kimura, Hideo

    2014-10-09

    Hydrogen sulfide (H2S) is recognized as a biological mediator with various roles such as neuromodulation, regulation of the vascular tone, cytoprotection, anti-inflammation, oxygen sensing, angiogenesis, and generation of mitochondrial energy. It is produced by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3MST). The activity of CBS is enhanced by S-adenosyl methionine (SAM) and glutathionylation, while it is inhibited by nitric oxide (NO) and carbon monoxide (CO). The activity of CSE and cysteine aminotransferase (CAT), which produces the 3MST substrate 3-mercaptopyruvate (3MP), is regulated by Ca2+. H2S is oxidized to thiosulfate in mitochondria through the sequential action of sulfide quinone oxidoreductase (SQR), sulfur dioxygenase, and rhodanese. The rates of the production and clearance of H2S determine its cellular concentration. Polysulfides (H2Sn) have been found to occur in the brain and activate transient receptor potential ankyrin 1 (TRPA1) channels, facilitate the translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) to the nucleus, and suppress the activity of phosphatase and tensin homolog (PTEN) by sulfurating (sulfhydrating) the target cysteine residues. A cross talk between H2S and NO also plays an important role in cardioprotection as well as regulation of the vascular tone. H2S, polysulfides, and their cross talk with NO may mediate various physiological and pathophysiological responses.

  8. Gaugino versus sfermion masses in gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Abel, Steven A., E-mail: s.a.abel@durham.ac.u [Institute for Particle Physics Phenomenology, Durham University, Durham DH1 3LE (United Kingdom); Jaeckel, Joerg, E-mail: joerg.jaeckel@durham.ac.u [Institute for Particle Physics Phenomenology, Durham University, Durham DH1 3LE (United Kingdom); Khoze, Valentin V., E-mail: valya.khoze@durham.ac.u [Institute for Particle Physics Phenomenology, Durham University, Durham DH1 3LE (United Kingdom)

    2010-01-04

    A well-known signature of supersymmetry breaking scenarios with ordinary gauge mediation is a universal formula governing gaugino and sfermion masses such that their ratio is of order one. On the other hand, recently studied models with direct gauge mediation predict anomalously small ratios of gaugino to scalar masses. It was argued that the smallness of gaugino masses is a consequence of being in the lowest energy state of the SUSY-breaking low energy effective theory. To increase gaugino masses one either has to move to higher metastable vacuum or alternatively remain in the original SUSY-breaking vacuum but extend the theory by introducing a lower-lying vacuum elsewhere. We follow the latter strategy and show that the ratio of gaugino to sfermion masses can be continuously varied between zero and of order one by bringing in a lower vacuum from infinity. We argue that the stability of the vacuum is directly linked to the ratio between the gaugino masses and the underlying SUSY-breaking scale, i.e. the gravitino mass.

  9. Depolarization-mediated regulation of alternative splicing

    Directory of Open Access Journals (Sweden)

    Alok eSharma

    2011-12-01

    Full Text Available Alternative splicing in eukaryotes plays an important role in regulating gene expression by selectively including alternative exons. A wealth of information has been accumulated that explains how alternative exons are selected in a developmental stage- or tissue-specific fashion. However, our knowledge of how cells respond to environmental changes to alter alternative splicing is very limited. For example, although a number of alternative exons have been shown to be regulated by calcium level alterations, the underlying mechanisms are not well understood. As calcium signaling in neurons plays a crucial role in essential neuronal functions such as learning and memory formation, it is important to understand how this process is regulated at every level in gene expression. The significance of the dynamic control of alternative splicing in response to changes of calcium levels has been largely unappreciated. In this communication, we will summarize the recent advances in calcium signaling-mediated alternative splicing that have provided some insights into the important regulatory mechanisms. In addition to describing the cis-acting RNA elements on the pre-mRNA molecules that respond to changes of intracellular calcium levels, we will summarize how splicing regulators change and affect alternative splicing in this process. We will also discuss a novel mode of calcium-mediated splicing regulation at the level of chromatin structure and transcription.

  10. Newborn Analgesia Mediated by Oxytocin during Delivery.

    Science.gov (United States)

    Mazzuca, Michel; Minlebaev, Marat; Shakirzyanova, Anastasia; Tyzio, Roman; Taccola, Giuliano; Janackova, Sona; Gataullina, Svetlana; Ben-Ari, Yehezkel; Giniatullin, Rashid; Khazipov, Rustem

    2011-01-01

    The mechanisms controlling pain in newborns during delivery are poorly understood. We explored the hypothesis that oxytocin, an essential hormone for labor and a powerful neuromodulator, exerts analgesic actions on newborns during delivery. Using a thermal tail-flick assay, we report that pain sensitivity is two-fold lower in rat pups immediately after birth than 2 days later. Oxytocin receptor antagonists strongly enhanced pain sensitivity in newborn, but not in 2-day-old rats, whereas oxytocin reduced pain at both ages suggesting an endogenous analgesia by oxytocin during delivery. Similar analgesic effects of oxytocin, measured as attenuation of pain-vocalization induced by electrical whisker pad stimulation, were also observed in decerebrated newborns. Oxytocin reduced GABA-evoked calcium responses and depolarizing GABA driving force in isolated neonatal trigeminal neurons suggesting that oxytocin effects are mediated by alterations of intracellular chloride. Unlike GABA signaling, oxytocin did not affect responses mediated by P2X3 and TRPV1 receptors. In keeping with a GABAergic mechanism, reduction of intracellular chloride by the diuretic NKCC1 chloride co-transporter antagonist bumetanide mimicked the analgesic actions of oxytocin and its effects on GABA responses in nociceptive neurons. Therefore, endogenous oxytocin exerts an analgesic action in newborn pups that involves a reduction of the depolarizing action of GABA on nociceptive neurons. Therefore, the same hormone that triggers delivery also acts as a natural pain killer revealing a novel facet of the protective actions of oxytocin in the fetus at birth.

  11. Ultrasound-Mediated Polymeric Micelle Drug Delivery.

    Science.gov (United States)

    Xia, Hesheng; Zhao, Yue; Tong, Rui

    2016-01-01

    The synthesis of multi-functional nanocarriers and the design of new stimuli-responsive means are equally important for drug delivery. Ultrasound can be used as a remote, non-invasive and controllable trigger for the stimuli-responsive release of nanocarriers. Polymeric micelles are one kind of potential drug nanocarrier. By combining ultrasound and polymeric micelles, a new modality (i.e., ultrasound-mediated polymeric micelle drug delivery) has been developed and has recently received increasing attention. A major challenge remaining in developing ultrasound-responsive polymeric micelles is the improvement of the sensitivity or responsiveness of polymeric micelles to ultrasound. This chapter reviews the recent advance in this field. In order to understand the interaction mechanism between ultrasound stimulus and polymeric micelles, ultrasound effects, such as thermal effect, cavitation effect, ultrasound sonochemistry (including ultrasonic degradation, ultrasound-initiated polymerization, ultrasonic in-situ polymerization and ultrasound site-specific degradation), as well as basic micellar knowledge are introduced. Ultrasound-mediated polymeric micelle drug delivery has been classified into two main streams based on the different interaction mechanism between ultrasound and polymeric micelles; one is based on the ultrasound-induced physical disruption of the micelle and reversible release of payload. The other is based on micellar ultrasound mechanochemical disruption and irreversible release of payload.

  12. Agrobacterium-mediated transformation of Fusarium proliferatum.

    Science.gov (United States)

    Bernardi-Wenzel, J; Quecine, M C; Azevedo, J L; Pamphile, J A

    2016-06-03

    Fusarium proliferatum is an important pathogen that is associated with plant diseases and primarily affects aerial plant parts by producing different mycotoxins, which are toxic to humans and animals. Within the last decade, this fungus has also been described as one of the causes of red root rot or sudden death syndrome in soybean, which causes extensive damage to this crop. This study describes the Agrobacterium tumefaciens-mediated transformation of F. proliferatum as a tool for the disruption of pathogenicity genes. The genetic transformation was performed using two binary vectors (pCAMDsRed and pFAT-GFP) containing the hph (hygromycin B resistance) gene as a selection marker and red and green fluorescence, respectively. The presence of acetosyringone and the use of filter paper or nitrocellulose membrane were evaluated for their effect on the transformation efficiency. A mean processing rate of 94% was obtained with 96 h of co-cultivation only in the presence of acetosyringone and the use of filter paper or nitrocellulose membrane did not affect the transformation process. Hygromycin B resistance and the presence of the hph gene were confirmed by PCR, and fluorescence due to the expression of GFP and DsRed protein was monitored in the transformants. A high rate of mitotic stability (95%) was observed. The efficiency of Agrobacterium-mediated transformation of F. proliferatum allows the technique to be used for random insertional mutagenesis studies and to analyze fungal genes involved in the infection process.

  13. Counterfactual Graphical Models for Longitudinal Mediation Analysis with Unobserved Confounding

    OpenAIRE

    Shpitser, Ilya

    2012-01-01

    Questions concerning mediated causal effects are of great interest in psychology, cognitive science, medicine, social science, public health, and many other disciplines. For instance, about 60% of recent papers published in leading journals in social psychology contain at least one mediation test (Rucker, Preacher, Tormala, & Petty, 2011). Standard parametric approaches to mediation analysis employ regression models, and either the "difference method" (Judd & Kenny, 1981), more common in epid...

  14. Mediated Electrochemical Measurements of Intracellular Catabolic Activities of Yeast Cells

    Institute of Scientific and Technical Information of China (English)

    Jin Sheng ZHAO; Zhen Yu YANG; Yao LU; Zheng Yu YANG

    2005-01-01

    Coupling with the dual mediator system menadione/ferricyanide, microelectrode voltammetric measurements were undertaken to detect the ferrocyanide accumulations arising from the mediated reduction of ferricyanide by yeast cells. The results indicate that the dual mediator system menadione/ferricyanide could be used as a probe to detect cellular catabolic activities in yeast cells and the electrochemical response has a positive relationship with the specific growth rate of yeast cells.

  15. Mediation skills for conflict resolution in nursing education.

    Science.gov (United States)

    Cheng, Fung Kei

    2015-07-01

    Encountering conflicts among family members in hospital produces burnout among nurses, implying a need for alternative dispute resolution training. However, current nursing education pays more attention to counselling skills training than to mediation. The present report examines the fundamental concepts of mediation, including its nature, basic assumptions and values, and compares those with counselling. Its implications may open a discussion on enhancing contemporary nursing education by providing mediation training in the workplace to nurses so that they can deal more effectively with disputes.

  16. VARIABEL LATEN SEBAGAI MODERATOR DAN MEDIATOR DALAM HUBUNGAN KAUSAL

    Directory of Open Access Journals (Sweden)

    I KOMANG GEDE ANTARA

    2014-01-01

    Full Text Available Latent variables are variables that can not be measured directly. In analysis of causal relationship involving three latent variables, one latent variable can be a moderator or mediator variables. Goodness of Fit moderation and mediation model of latent variables is affected by the value of the canonical correlation between moderator/mediator latent variables with the independent latent variables and dependent latent variables. If the value of both canonical correlation is well , so the Goodness of Fit models of mediation is getting better, while the opposite Goodness of Fit models will be better moderation.

  17. Let's talk conflict: using mediation in healthcare security disputes.

    Science.gov (United States)

    Armstrong, Brad

    2013-01-01

    Healthcare security administrators face weekly, if not daily challenges and conflict. This article considers that security leaders must anticipate disputes in highly complex healthcare systems. When disputes cannot be resolved by organizational efforts, security administrators may be in a position to recommend or participate in mediation. Here the concept of mediation is introduced to healthcare security leaders as a viable means to resolve disputes with patients, visitors, and the community. This includes a description of the facilitative versus evaluative mediation processes, as well as pragmatic guidance when preparing for mediation.

  18. The role of mediation in resolving workplace relationship conflict.

    Science.gov (United States)

    McKenzie, Donna Margaret

    2015-01-01

    Stress triggered by workplace-based interpersonal conflict can result in damaged relationships, loss of productivity, diminished job satisfaction and increasingly, workers' compensation claims for psychological injury. This paper examined the literature on the role and effectiveness of mediation, as the most common method of Alternative Dispute Resolution, in resolving workplace relationship conflict. Available evidence suggests that mediation is most effective when supported by organisational commitment to ADR strategies, policies and processes, and conducted by independent, experienced and qualified mediators. The United States Postal Service program REDRESS™ is described as an illustration of the successful use of mediation to resolve conflict in the workplace.

  19. A Critique of Private Sessions in Family Mediation

    Directory of Open Access Journals (Sweden)

    Don Bowen

    2013-02-01

    Full Text Available This study represents a critical examination of private sessions (caucuses, seen as a sub-process within the core family mediation process and defined as involving separate and confidential conversations between mediator(s and each disputant during the main session. In the study, the views of family mediators were explored revealing that considerable support for the use of the tool was juxtaposed with a range of fundamental ethical concerns. Emerging from the study is strong evidence of coercive practice as an inherent component of caucusing thus posing a challenge to the positive benefits.

  20. Mediation involving a juvenile perpetrators of criminal acts

    Directory of Open Access Journals (Sweden)

    Małgorzata Cwalina

    2012-12-01

    Full Text Available The article describes issues concerning mediatory proceedings regarding juvenile court cases in Poland. It was based on original master’s dissertation entitled “Mediation as a form of protection of the juvenile against stigma”. Mediation is most often defined as a voluntary attempt to resolve the conflict and to reach an agreement as it regards the compensation, in presence of impartial and neutral mediator. By contrast with court cases, mediation’s aim is not to point out who is right, but to find a solution of conflict, satisfactory to all parties. Fundamental aim of mediation within juvenile cases is educational and reformatory influence.

  1. Mediation analyses: applications in nutrition research and reading the literature.

    Science.gov (United States)

    Lockwood, Chondra M; DeFrancesco, Carol A; Elliot, Diane L; Beresford, Shirley A A; Toobert, Deborah J

    2010-05-01

    Mediation analysis is a newer statistical tool that is becoming more prominent in nutrition research. Its use provides insight into the relationship among variables in a potential causal chain. For intervention studies, it can define the influence of different programmatic components and, in doing so, allows investigators to identify and refine a program's critical aspects. We present an overview of mediation analysis, compare mediators with other variables (confounders, moderators, and covariates), and illustrate how mediation analysis permits interpretation of the change process. A framework is outlined for the critical appraisal of articles purporting to use mediation analysis. The framework's utility is demonstrated by searching the nutrition literature and identifying articles citing mediation cross referenced with the terms "nutrition," "diet," "food," and "obesity." Seventy-two articles were identified that involved human subjects and behavior outcomes, and almost half mentioned mediation without tests to define its presence. Tabulation of the 40 articles appropriately assessing mediation demonstrates an increase in these techniques' appearance and the breadth of nutrition topics addressed. Mediation analysis is an important new statistical tool. Familiarity with its methodology and a framework for assessing articles will allow readers to critically appraise the literature and make informed independent evaluations of works using these techniques.

  2. PIC Activation through Functional Interplay between Mediator and TFIIH.

    Science.gov (United States)

    Malik, Sohail; Molina, Henrik; Xue, Zhu

    2017-01-06

    The multiprotein Mediator coactivator complex functions in large part by controlling the formation and function of the promoter-bound preinitiation complex (PIC), which consists of RNA polymerase II and general transcription factors. However, precisely how Mediator impacts the PIC, especially post-recruitment, has remained unclear. Here, we have studied Mediator effects on basal transcription in an in vitro transcription system reconstituted from purified components. Our results reveal a close functional interplay between Mediator and TFIIH in the early stages of PIC development. We find that under conditions when TFIIH is not normally required for transcription, Mediator actually represses transcription. TFIIH, whose recruitment to the PIC is known to be facilitated by the Mediator, then acts to relieve Mediator-induced repression to generate an active form of the PIC. Gel mobility shift analyses of PICs and characterization of TFIIH preparations carrying mutant XPB translocase subunit further indicate that this relief of repression is achieved through expending energy via ATP hydrolysis, suggesting that it is coupled to TFIIH's established promoter melting activity. Our interpretation of these results is that Mediator functions as an assembly factor that facilitates PIC maturation through its various stages. Whereas the overall effect of the Mediator is to stimulate basal transcription, its initial engagement with the PIC generates a transcriptionally inert PIC intermediate, which necessitates energy expenditure to complete the process.

  3. Bacteria-mediated bisphenol A degradation.

    Science.gov (United States)

    Zhang, Weiwei; Yin, Kun; Chen, Lingxin

    2013-07-01

    Bisphenol A (BPA) is an important monomer in the manufacture of polycarbonate plastics, food cans, and other daily used chemicals. Daily and worldwide usage of BPA and BPA-contained products led to its ubiquitous distribution in water, sediment/soil, and atmosphere. Moreover, BPA has been identified as an environmental endocrine disruptor for its estrogenic and genotoxic activity. Thus, BPA contamination in the environment is an increasingly worldwide concern, and methods to efficiently remove BPA from the environment are urgently recommended. Although many factors affect the fate of BPA in the environment, BPA degradation is mainly depended on the metabolism of bacteria. Many BPA-degrading bacteria have been identified from water, sediment/soil, and wastewater treatment plants. Metabolic pathways of BPA degradation in specific bacterial strains were proposed, based on the metabolic intermediates detected during the degradation process. In this review, the BPA-degrading bacteria were summarized, and the (proposed) BPA degradation pathway mediated by bacteria were referred.

  4. Masking mediated print defect visibility predictor

    Science.gov (United States)

    Jing, Xiaochen; Nachlieli, Hila; Shaked, Doron; Shiffman, Smadar; Allebach, Jan P.

    2012-01-01

    Banding is a well-known artifact produced by printing systems. It usually appears as lines perpendicular to the process direction of the print. Therefore, banding is an important print quality issue which has been analyzed and assessed by many researchers. However, little literature has focused on the study of the masking effect of content for this kind of print quality issue. Compared with other image and print quality research, our work is focused on the print quality of typical documents printed on a digital commercial printing press. In this paper, we propose a Masking Mediated Print Defect Visibility Predictor (MMPDVP) to predict the visibility of defects in the presence of customer content. The parameters of the algorithm are trained from ground-truth images that have been marked by subjects. The MMPDVP could help the press operator decide whether the print quality is acceptable for specific customer requirements. Ultimately, this model can be used to optimize the print-shop workflow.

  5. General gauge mediation at the weak scale

    Science.gov (United States)

    Knapen, Simon; Redigolo, Diego; Shih, David

    2016-03-01

    We completely characterize General Gauge Mediation (GGM) at the weak scale by solving all IR constraints over the full parameter space. This is made possible through a combination of numerical and analytical methods, based on a set of algebraic relations among the IR soft masses derived from the GGM boundary conditions in the UV. We show how tensions between just a few constraints determine the boundaries of the parameter space: electroweak symmetry breaking (EWSB), the Higgs mass, slepton tachyons, and left-handed stop/sbottom tachyons. While these constraints allow the left-handed squarks to be arbitrarily light, they place strong lower bounds on all of the right-handed squarks. Meanwhile, light EW superpartners are generic throughout much of the parameter space. This is especially the case at lower messenger scales, where a positive threshold correction to m h coming from light Higgsinos and winos is essential in order to satisfy the Higgs mass constraint.

  6. Mast Cell-Mediated Mechanisms of Nociception.

    Science.gov (United States)

    Aich, Anupam; Afrin, Lawrence B; Gupta, Kalpna

    2015-12-04

    Mast cells are tissue-resident immune cells that release immuno-modulators, chemo-attractants, vasoactive compounds, neuropeptides and growth factors in response to allergens and pathogens constituting a first line of host defense. The neuroimmune interface of immune cells modulating synaptic responses has been of increasing interest, and mast cells have been proposed as key players in orchestrating inflammation-associated pain pathobiology due to their proximity to both vasculature and nerve fibers. Molecular underpinnings of mast cell-mediated pain can be disease-specific. Understanding such mechanisms is critical for developing disease-specific targeted therapeutics to improve analgesic outcomes. We review molecular mechanisms that may contribute to nociception in a disease-specific manner.

  7. Symbiont-mediated RNA interference in insects.

    Science.gov (United States)

    Whitten, Miranda M A; Facey, Paul D; Del Sol, Ricardo; Fernández-Martínez, Lorena T; Evans, Meirwyn C; Mitchell, Jacob J; Bodger, Owen G; Dyson, Paul J

    2016-02-24

    RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects.

  8. Epigenetics and therapeutic targets mediating neuroprotection.

    Science.gov (United States)

    Qureshi, Irfan A; Mehler, Mark F

    2015-12-02

    The rapidly evolving science of epigenetics is transforming our understanding of the nervous system in health and disease and holds great promise for the development of novel diagnostic and therapeutic approaches targeting neurological diseases. Increasing evidence suggests that epigenetic factors and mechanisms serve as important mediators of the pathogenic processes that lead to irrevocable neural injury and of countervailing homeostatic and regenerative responses. Epigenetics is, therefore, of considerable translational significance to the field of neuroprotection. In this brief review, we provide an overview of epigenetic mechanisms and highlight the emerging roles played by epigenetic processes in neural cell dysfunction and death and in resultant neuroprotective responses. This article is part of a Special Issue entitled SI: Neuroprotection.

  9. Brain Pericytes As Mediators of Neuroinflammation.

    Science.gov (United States)

    Rustenhoven, Justin; Jansson, Deidre; Smyth, Leon C; Dragunow, Mike

    2017-03-01

    Brain pericytes are perivascular cells that regulate capillary function, and this localization puts them in a pivotal position for the regulation of central nervous system (CNS) inflammatory responses at the neurovascular unit. Neuroinflammation, driven by microglia and astrocytes or resulting from peripheral leukocyte infiltration, has both homeostatic and detrimental consequences for brain function and is present in nearly every neurological disorder. More recently, brain pericytes have been shown to have many properties of immune regulating cells, including responding to and expressing a plethora of inflammatory molecules, presenting antigen, and displaying phagocytic ability. In this review we highlight the emerging role of pericytes in neuroinflammation and discuss pericyte-mediated neuroinflammation as a potential therapeutic target for the treatment of a range of devastating brain disorders.

  10. Exosomes: mediators of communication in eukaryotes.

    Science.gov (United States)

    Lopez-Verrilli, María A; Court, Felipe A

    2013-01-01

    In addition to the established mechanisms of intercellular signaling, a new way of communication has gained much attention in the last decade: communication mediated by exosomes. Exosomes are nanovesicles (with a diameter of 40-120 nm) secreted into the extracellular space by the multivesicular endosome after its outer membrane fuses with the plasma membrane. Once released, exosomes modulate the response of the recipient cells that recognize them. This indicates that exosomes operate in a specific manner and participate in the regulation of the target cell. Remarkably, exosomes occur from unicellular organisms to mammals, suggesting an evolutionarily conserved mechanism of communication. In this review we describe the cascade of exosome formation, intracellular traffic, secretion, and internalization by recipient cells, and review their most relevant effects. We also highlight important steps that are still poorly understood.

  11. Professional networking using computer-mediated communication.

    Science.gov (United States)

    Washer, Peter

    Traditionally, professionals have networked with others in their field through attending conferences, professional organizations, direct mailing, and via the workplace. Recently, there have been new possibilities to network with other professionals using the internet. This article looks at the possibilities that the internet offers for professional networking, particularly e-mailing lists, newsgroups and membership databases, and compares them against more traditional methods of professional networking. The different types of computer-mediated communication are discussed and their relative merits and disadvantages are examined. The benefits and potential pitfalls of internet professional networking, as it relates to the nursing profession, are examined. Practical advice is offered on how the internet can be used as a means to foster professional networks of academic, clinical or research interests.

  12. Iron: a pathological mediator of Alzheimer disease?

    Science.gov (United States)

    Bishop, Glenda M; Robinson, Stephen R; Liu, Quan; Perry, George; Atwood, Craig S; Smith, Mark A

    2002-01-01

    Brains from patients with Alzheimer disease (AD) show a disruption in the metabolism of iron, such that there is an accumulation of iron in senile plaques, and an altered distribution of iron transport and storage proteins. One of the earliest events in AD is the generation of oxidative stress, which may be related to the generation of free radicals by the excess iron that is observed in the disease. Iron has also been shown to mediate the in vitro toxicity of amyloid-beta peptide, and the presence of iron in most in vitro systems could underlie the toxicity that is normally attributed to amyloid-beta in these studies. In contrast, several recent studies have suggested that amyloid-beta may decrease oxidative stress and decrease the toxicity of iron. Continued examination of the complex interactions that occur between iron and amyloid-beta may assist in the elucidation of the mechanisms that underlie the neurodegeneration that leads to dementia in AD.

  13. Catalytic Organic Transformations Mediated by Actinide Complexes

    Directory of Open Access Journals (Sweden)

    Isabell S. R. Karmel

    2015-10-01

    Full Text Available This review article presents the development of organoactinides and actinide coordination complexes as catalysts for homogeneous organic transformations. This chapter introduces the basic principles of actinide catalysis and deals with the historic development of actinide complexes in catalytic processes. The application of organoactinides in homogeneous catalysis is exemplified in the hydroelementation reactions, such as the hydroamination, hydrosilylation, hydroalkoxylation and hydrothiolation of alkynes. Additionally, the use of actinide coordination complexes for the catalytic polymerization of α-olefins and the ring opening polymerization of cyclic esters is presented. The last part of this review article highlights novel catalytic transformations mediated by actinide compounds and gives an outlook to the further potential of this field.

  14. Gauge Mediation Models with Adjoint Messengers

    CERN Document Server

    Gogoladze, Ilia; Shafi, Qaisar; Un, Cem Salih

    2016-01-01

    We present a class of models in the framework of gauge mediation supersymmetry breaking where the messenger fields transform in the adjoint representation of the Standard Model gauge symmetry. To avoid unacceptably light right-handed sleptons in the spectrum we introduce a non-zero U(1)_B-L D-term. This leads to an additional contribution to the soft supersymmetry breaking mass terms which makes the right-handed slepton masses compatible with the current experimental bounds. We show that in this framework the observed 125 GeV Higgs boson mass can be accommodated with the sleptons accessible at the LHC, while the squarks and gluinos lie in the multi-TeV range. We also discuss the issue of the fine-tuning and show that the desired relic dark matter abundance can also be accommodated.

  15. Cytokine and lipid mediator networks in tuberculosis.

    Science.gov (United States)

    Mayer-Barber, Katrin D; Sher, Alan

    2015-03-01

    A major approach for immunologic intervention in tuberculosis involves redirecting the outcome of the host immune response from the induction of disease to pathogen control. Cytokines and lipid mediators known as eicosanoids play key roles in regulating this balance and as such represent important targets for immunologic intervention. While the evidence for cytokine/eicosanoid function derives largely from the investigation of murine and zebrafish experimental infection models, clinical studies have confirmed the existence of many of the same pathways in tuberculosis patients. Here, we summarize new data that reveal important intersections between the cytokine and eicosanoid networks in the host response to mycobacteria and discuss how targeting this crosstalk can promote resistance to lethal Mycobacterium tuberculosis infection. This approach could lead to new host-directed therapies to be used either as an adjunct for improving the efficacy of standard antibiotic treatment or for the management of drug-resistant infections.

  16. Molecular Mechanisms of Lymphocyte-Mediated Cytotoxicity

    Institute of Scientific and Technical Information of China (English)

    Zusen Fan; Qixiang Zhang

    2005-01-01

    Granule-mediated cytotoxicity is the major mechanism for lymphocytes to kill viruses, intracellular bacteria and tumors. The cytotoxic granules move to the immunological synapse by exocytosis after recognition of a killer cell.The contents of the granules are delivered into target cells with the help of perforin by endocytosis. A group of serine protease granzymes cleave their critical substrates to initiate DNA damage and cell death. The most abundant granzymes are granzyme A and B. They induce cell death through alternate and nonoverlapping pathways. The substrates and functions of the majority of the orphan granzymes have not yet been identified. It is possible that the diversity of granzymes provides fail-safe mechanisms for killing viruses and tumor cells.

  17. ROS and ROS-Mediated Cellular Signaling

    Directory of Open Access Journals (Sweden)

    Jixiang Zhang

    2016-01-01

    Full Text Available It has long been recognized that an increase of reactive oxygen species (ROS can modify the cell-signaling proteins and have functional consequences, which successively mediate pathological processes such as atherosclerosis, diabetes, unchecked growth, neurodegeneration, inflammation, and aging. While numerous articles have demonstrated the impacts of ROS on various signaling pathways and clarify the mechanism of action of cell-signaling proteins, their influence on the level of intracellular ROS, and their complex interactions among multiple ROS associated signaling pathways, the systemic summary is necessary. In this review paper, we particularly focus on the pattern of the generation and homeostasis of intracellular ROS, the mechanisms and targets of ROS impacting on cell-signaling proteins (NF-κB, MAPKs, Keap1-Nrf2-ARE, and PI3K-Akt, ion channels and transporters (Ca2+ and mPTP, and modifying protein kinase and Ubiquitination/Proteasome System.

  18. Phenomenology of deflected anomaly-mediation

    CERN Document Server

    Rattazzi, Riccardo; Wells, J D; Rattazzi, Riccardo; Strumia, Alessandro; Wells, James D.

    2000-01-01

    We explore the phenomenology of a class of models with anomaly-mediated supersymmetry breaking. These models retain the successful flavor properties of the minimal scenario while avoiding the tachyons. The mass spectrum is predicted in terms of a few parameters. However various qualitatively different spectra are possible, often strongly different from the ones usually employed to explore capabilities of new accelerators. One stable feature is the limited spread of the spectrum, so that squarks and gluinos could be conceivably produced at TEVII. The lightest superpartner of standard particles is often a charged slepton or a neutral higgsino. It behaves as a stable particle in collider experiments but it decays at or before nucleosynthesis. We identify the experimental signatures at hadron colliders that can help distinguish this scenario from the usual ones.

  19. Magnetically mediated vortexlike assembly of gold nanoshells.

    Science.gov (United States)

    Sun, Jianfei; Dong, Jian; Sun, Dongke; Guo, Zhirui; Gu, Ning

    2012-04-24

    Gold nanoshells currently attract increasing research interests due to the important role in many subjects. For practical applications, random arrangement of the nanoparticles is often unfavored so that the assembly of gold nanoshells is becoming a central issue. We here proposed to utilize time-variant magnetic field to direct the assembly of gold nanoshells. It was discovered that the alternating magnetic field can mediate the vortex-like assembly of gold nanoshells. The mechanism was explored and thought to be relative with the electric field of induction which caused the thermal gradient on the substrate and the electric force. The vortexlike structure as well as the assembly mechanism will play an important role in research and application of gold nanomaterials.

  20. Modelling toehold-mediated RNA strand displacement

    CERN Document Server

    Šulc, Petr; Romano, Flavio; Doye, Jonathan P K; Louis, Ard A

    2014-01-01

    We study the thermodynamics and kinetics of an RNA toehold-mediated strand displacement reaction with a recently developed coarse-grained model of RNA. Strand displacement, during which a single strand displaces a different strand previously bound to a complementary substrate strand, is an essential mechanism in active nucleic acid nanotechnology and has also been hypothesized to occur in vivo. We study the rate of displacement reactions as a function of the length of the toehold and temperature and make two experimentally testable predictions: that the displacement is faster if the toehold is placed at the 5' end of the substrate and that the displacement slows down with increasing temperature for longer toeholds.

  1. Agrobacterium-mediated transformation of Brachypodium distachyon.

    Science.gov (United States)

    Thole, Vera; Vain, Philippe

    2012-01-01

    Brachypodium distachyon is an attractive genomics and biological model system for grass research. Recently, the complete annotated genome sequence of the diploid line Bd21 has been released. Genetic transformation technologies are critical for the discovery and validation of gene function in Brachypodium. Here, we describe an efficient procedure enabling the Agrobacterium-mediated transformation of a range of diploid and polyploid genotypes of Brachypodium. The procedure relies on the transformation of compact embryogenic calli derived from immature embryos using either chemical selection alone or a combination of chemical and visual screening of transformed tissues and plants. Transformation efficiencies of around 20% can routinely be achieved using this protocol. In the context of the BrachyTAG programme (BrachyTAG.org), this procedure made possible the mass production of Bd21T-DNA mutant plant lines.

  2. The anglicisms in computer-mediated communication

    Directory of Open Access Journals (Sweden)

    Jan Lazar

    2012-01-01

    Full Text Available Although French is a Romance language descendant from the Latin, there is of course some influence of other languages on it. English is perhaps the most important source of loan-words for the present French language. Our article is focused on new forms of written communication, mainly computer-mediated communication (CMC. The main aim of this article is to analyze the loan-words, especially the anglicisms that are used by chatters in various French chats. After examining the motivations of loan, the article studies the frequency of anglicisms in three chats and observes their grammatical adaptation in the context of CMC. A huge richness of anglicisms is illustrated by concrete examples taken from our corpus.

  3. General Gauge Mediation at the Weak Scale

    CERN Document Server

    Knapen, Simon; Shih, David

    2015-01-01

    We completely characterize General Gauge Mediation (GGM) at the weak scale by solving all IR constraints over the full parameter space. This is made possible through a combination of numerical and analytical methods, based on a set of algebraic relations among the IR soft masses derived from the GGM boundary conditions in the UV. We show how tensions between just a few constraints determine the boundaries of the parameter space: electroweak symmetry breaking (EWSB), the Higgs mass, slepton tachyons, and left-handed stop/sbottom tachyons. While these constraints allow the left-handed squarks to be arbitrarily light, they place strong lower bounds on all of the right-handed squarks. Meanwhile, light EW superpartners are generic throughout much of the parameter space. This is especially the case at lower messenger scales, where a positive threshold correction to $m_h$ coming from light Higgsinos and winos is essential in order to satisfy the Higgs mass constraint.

  4. Nacnac‐Cobalt‐Mediated P4 Transformations

    Science.gov (United States)

    Spitzer, Fabian; Graßl, Christian; Balázs, Gábor; Mädl, Eric; Keilwerth, Martin; Zolnhofer, Eva M.; Meyer, Karsten

    2017-01-01

    Abstract A comparison of P4 activations mediated by low‐valent β‐diketiminato (L) cobalt complexes is presented. The formal Co0 source [K2(L3Co)2(μ2:η1,η1‐N2)] (1) reacts with P4 to form a mixture of the monoanionic complexes [K(thf)6][(L3Co)2(μ2:η4,η4‐P4)] (2) and [K(thf)6][(L3Co)2(μ2:η3,η3‐P3)] (3). The analogue CoI precursor [L3Co(tol)] (4 a), however, selectively yields the corresponding neutral derivative [(L3Co)2(μ2:η4,η4‐P4)] (5 a). Compound 5 a undergoes thermal P atom loss to form the unprecedented complex [(L3Co)2(μ2:η3,η3‐P3)] (6). The products 2 and 3 can be obtained selectively by an one‐electron reduction of their neutral precursors 5 a and 6, respectively. The electrochemical behaviour of 2, 3, 5 a, and 6 is monitored by cyclic voltammetry and their magnetism is examined by SQUID measurements and the Evans method. The initial CoI‐mediated P4 activation is not influenced by applying the structurally different ligands L1 and L2, which is proven by the formation of the isostructural products [(LCo)2(μ2:η4,η4‐P4)] [L=L3 (5 a), L1 (5 b), L2 (5 c)]. PMID:28032678

  5. Restriction enzyme-mediated DNA family shuffling.

    Science.gov (United States)

    Behrendorff, James B Y H; Johnston, Wayne A; Gillam, Elizabeth M J

    2014-01-01

    DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without primers. The fragments anneal where there is sufficient sequence identity, resulting in full-length variants of the original gene that have inherited mutations from multiple templates. Subsequent studies demonstrated that directed evolution could be further accelerated by shuffling similar native protein-coding sequences from the same gene family, rather than mutated variants of a single gene. Generally at least 65-75 % global identity between parental sequences is required in DNA family shuffling, with recombination mostly occurring at sites with at least five consecutive nucleotides of local identity. Since DNA shuffling was originally developed, many variations on the method have been published. In particular, the use of restriction enzymes in the fragmentation step allows for greater customization of fragment lengths than DNase I digestion and avoids the risk that parental sequences may be over-digested into unusable very small fragments. Restriction enzyme-mediated fragmentation also reduces the occurrence of undigested parental sequences that would otherwise reduce the number of unique variants in the resulting library. In the current chapter, we provide a brief overview of the alternative methods currently available for DNA shuffling as well as a protocol presented here that improves on several previous implementations of restriction enzyme-mediated DNA family shuffling, in particular with regard to purification of DNA fragments for reassembly.

  6. Silver- and gold-mediated nucleobase bonding.

    Science.gov (United States)

    Acioli, Paulo H; Srinivas, Sudha

    2014-08-01

    We report the results of a density functional theory investigation of the bonding of nucleobases mediated by silver and gold atoms in the gas phase. Our calculations use the Becke exchange and Perdew-Wang correlation functional (BPW91) combined with the Stuttgart effective core potentials to represent the valence electrons of gold, silver, and platinum, and the all-electron DGTZVP basis set for C, H, N, and O. This combination was chosen based on tests on the metal atoms and tautomers of adenine, cytosine, and guanine. To establish a benchmark to understand the metal-mediated bonding, we calculated the binding energy of each of the base pairs in their canonical forms. Our calculations show rather strong bonds between the Watson-Crick base pairs when compared with typical values for N-H-N and N-H-O hydrogen bonds. The neutral metal atoms tend to bond near the nitrogen atoms. The effect of the metal atoms on the bonding of nucleobases differs depending on whether or not the metal atoms bond to one of the hydrogen-bonding sites. When the silver or gold atoms bond to a non-hydrogen-bonding site, the effect is a slight enhancement of the cytosine-guanine bonding, but there is almost no effect on the adenine-thymine pairing. The metal atoms can block one of the hydrogen-bonding sites, thus preventing the normal cytosine-guanine and adenine-thymine pairings. We also find that both silver and gold can bond to consecutive guanines in a similar fashion to platinum, albeit with a significantly lower binding energy.

  7. AFRICAN-STYLE MEDIATION AND WESTERN-STYLE DIVORCE AND FAMILY MEDIATION: REFLECTIONS FOR THE SOUTH AFRICAN CONTEXT

    Directory of Open Access Journals (Sweden)

    AE Boniface

    2012-12-01

    Full Text Available Both Western-styled mediation and African-styled mediation are practised in South Africa. Each of these models is applied in specific social contexts. In this article a brief explanation of what is meant by the term divorce and family mediation is provided. Thereafter the principles and processes of both Western-styled divorce and family mediation and African-styled group mediation are explored. Attention is given to the roles of mediators in both of these models as well as the ubuntu-styled values found in African group mediation. In Africa, there is a tradition of family neighbourhood negotiation facilitated by elders and an attitude of togetherness in the spirit of humanhood. Both of these show a commitment to the community concerned and a comprehensive view of life. In Africa conflicts are viewed as non-isolated events and are viewed in their social contexts. Not only are consequences for the disputing parties taken into account but also consequences for others in their families. These methods can be found in present-day methods, which are either used independently of imported Western structures or used alternatively to such structures. In this article the concept of mediation circles, as currently found in Western-styled mediation are also covered. Additionally, the provisions of the Children’s Act 38 of 2005 referring to mediation as well as the provisions of the Child Justice Act 75 of 2008 and family group conferencing in the realm of restorative justice in South Africa are critiqued. It is suggested that divorce and family mediation can learn from the principles of restorative justice applied during family group conferencing as well as from African-styled group mediation.

  8. Technological, mediatic and cultural hybridisation: Cultural mediations in the context of globalisation

    Directory of Open Access Journals (Sweden)

    Laan Mendes de Barros

    2009-12-01

    Full Text Available We live in a context of borders that are dissolving in many senses, of the convergence and hybridisation of technologies, mass media and cultures. The context is the resizing of practical time, of movements and links between the local and the global. In these times of interculturality, communication plays a very important role; not so much in its technological media dimension, but particularly in the dynamics of cultural mediations that are dividing off from mediatised relations. This article aims to reflect on the transformations in present-day communication processes, marked by strong movements of hybridisation, as well as examining how to consider interculturality in the context of cultural mediations, based on dialogue between Latin American and French authors. Also, using media material, the article presents illustrations of the Brazilian cultural scene, which is marked by a long history of hybridisation that is filled with intercultural dynamics.

  9. Love and Marital Quality in Romantic Relationships Mediated and Non-Mediated by Internet

    Directory of Open Access Journals (Sweden)

    Karla Rafaela Haack

    2014-04-01

    Full Text Available Love is one of the most desired feelings by human beings. According to the triangular theory of love, it is composed of three elements: intimacy, passion and decision/commitment, which constitutes a dimension related to the quality of love the partners perceive in their relationship. This study aims to compare the three elements of love and marital quality of 86 Internet users (43 in a relationship mediated by the Internet and 43 with a physical relationship. The results indicate that there is a significant difference in the intimacy, the decision/commitment and the whole dimension of love, as well as in the marital quality between partners who have a relationship mediated by Internet and the ones in a physical relationship. We can conclude that the Internet is a great tool for people to meet, but that it is important for the relationship to develop in a face-to-face context.

  10. miRNA-mediated auxin signalling repression during Vat-mediated aphid resistance in Cucumis melo.

    Science.gov (United States)

    Sattar, Sampurna; Addo-Quaye, Charles; Thompson, Gary A

    2016-06-01

    Resistance to Aphis gossypii in melon is attributed to the presence of the single dominant R gene virus aphid transmission (Vat), which is biologically expressed as antibiosis, antixenosis and tolerance. However, the mechanism of resistance is poorly understood at the molecular level. Aphid-induced transcriptional changes, including differentially expressed miRNA profiles that correspond to resistance interaction have been reported in melon. The potential regulatory roles of miRNAs in Vat-mediated aphid resistance were further revealed by identifying the specific miRNA degradation targets. A total of 70 miRNA:target pairs, including 28 novel miRNA:target pairs, for the differentially expressed miRNAs were identified: 11 were associated with phytohormone regulation, including six miRNAs that potentially regulate auxin interactions. A model for a redundant regulatory system of miRNA-mediated auxin insensitivity is proposed that incorporates auxin perception, auxin modification and auxin-regulated transcription. Chemically inhibiting the transport inhibitor response-1 (TIR-1) auxin receptor in susceptible melon tissues provides in vivo support for the model of auxin-mediated impacts on A. gossypii resistance.

  11. A Theory of Electronic Propinquity: Mediated Communication in Organizations

    Science.gov (United States)

    Korzenny, Felipe

    1978-01-01

    Proposes a theoretical approach to mediated communication in organizations suggesting that man-machine interface in mediated human communication is more effectively dealt with by using a comprehensive theoretical approach rather than separate communication devices that are tested as they appear in the market. (MH)

  12. Impact of mass generation for spin-1 mediator simplified models

    Science.gov (United States)

    Bell, Nicole F.; Cai, Yi; Leane, Rebecca K.

    2017-01-01

    In the simplified dark matter models commonly studied, the mass generation mechanism for the dark fields is not typically specified. We demonstrate that the dark matter interaction types, and hence the annihilation processes relevant for relic density and indirect detection, are strongly dictated by the mass generation mechanism chosen for the dark sector particles, and the requirement of gauge invariance. We focus on the class of models in which fermionic dark matter couples to a spin-1 vector or axial-vector mediator. However, in order to generate dark sector mass terms, it is necessary in most cases to introduce a dark Higgs field and thus a spin-0 scalar mediator will also be present. In the case that all the dark sector fields gain masses via coupling to a single dark sector Higgs field, it is mandatory that the axial-vector coupling of the spin-1 mediator to the dark matter is non-zero; the vector coupling may also be present depending on the charge assignments. For all other mass generation options, only pure vector couplings between the spin-1 mediator and the dark matter are allowed. If these coupling restrictions are not obeyed, unphysical results may be obtained such as a violation of unitarity at high energies. These two-mediator scenarios lead to important phenomenology that does not arise in single mediator models. We survey two-mediator dark matter models which contain both vector and scalar mediators, and explore their relic density and indirect detection phenomenology.

  13. 77 FR 23208 - Assessment of Mediation and Arbitration Procedures

    Science.gov (United States)

    2012-04-18

    ... FR 19,591). The Board favors the resolution of disputes through the use of mediation and arbitration... of information described below and in greater detail at 77 FR 19,591 is necessary for the proper... Surface Transportation Board 49 CFR Parts 1108 and 1109 Assessment of Mediation and Arbitration...

  14. SPECIAL ISSUES REGARDING THE MEDIATION PROCESS IN THE COMMERCIAL FIELD

    Directory of Open Access Journals (Sweden)

    SEPTIMIU STOICA

    2012-05-01

    Full Text Available The purpose of the research subject to this paper is to find proper solutions for the increase of the volume and efficiency in the field of commercial mediation. Starting from practical remarks, after an analysis of the substance, a new concept is suggested to be included and put into operation, namely the special commercial mediation, and a plead is made for its use in the current activity of alternative dispute resolution. The first major objective of this paper is to demonstrate once again the need and utility of mediation in the commercial field, insisting though on its remarkable specific nature. The second objective is, starting from the outlined differences between the commercial mediation and the classical mediation, to define and to conceptualize the special commercial mediation, as a separate branch of mediation. The third objective of this paper is to draw the regime of the newly defined category of commercial mediation from a regulatory point of view, of the implementation structure and techniques as foreseen.

  15. 78 FR 29071 - Assessment of Mediation and Arbitration Procedures

    Science.gov (United States)

    2013-05-17

    ...\\ Assessment of Mediation and Arbitration Procedures, 75 FR 52054. \\4\\ Assessment of Mediation and Arbitration... Resolution, 60 FR 19494, 19499-500 (April 19, 1995) (codified at 18 CFR 385.605 (Rule 605)) (describing FERC... and Arbitration Procedures AGENCY: Surface Transportation Board, DOT. ACTION: Final rules....

  16. Networked Mobilities and new sites of mediated interaction

    DEFF Research Database (Denmark)

    Jensen, Ole B.

    2008-01-01

    as the relationship to sites and places. Furthermore, an increasing number of such mobile practices are mediated by technologies of tangible and less tangible sorts. The paper concludes with a research agenda for unfolding a ‘politics of visibility', engaging with the ambivalences of networked mobilities and mediated...

  17. Mediating Variables in a Transtheoretical Model Dietary Intervention Program

    Science.gov (United States)

    Di Noia, Jennifer; Prochaska, James O.

    2010-01-01

    This study identified mediators of a Transtheoretical Model (TTM) intervention to increase fruit and vegetable consumption among economically disadvantaged African American adolescents (N = 549). Single-and multiple-mediator models were used to determine whether pros, cons, self-efficacy, and stages of change satisfied four conclusions necessary…

  18. Emotions and Young Offenders' Suitability for Victim-Offender Mediation.

    Science.gov (United States)

    Vieira, Tracey A.

    Although evidence indicates that Victim-Offender Mediation (VOM) provides an effective alternative to traditional sanctioning for young offenders, research investigating suitable candidates for VOM is lacking. Reintegrative shaming is theorized to be the mechanism underlying successful mediation; however, it is difficult to determine whether shame…

  19. School Processes Mediate School Compositional Effects: Model Specification and Estimation

    Science.gov (United States)

    Liu, Hongqiang; Van Damme, Jan; Gielen, Sarah; Van Den Noortgate, Wim

    2015-01-01

    School composition effects have been consistently verified, but few studies ever attempted to study how school composition affects school achievement. Based on prior research findings, we employed multilevel mediation modeling to examine whether school processes mediate the effect of school composition upon school outcomes based on the data of 28…

  20. Technological Mediation and Power: Postphenomenology, Critical Theory, and Autonomist Marxism

    NARCIS (Netherlands)

    Bantwal Rao, M.; Jongerden, J.P.; Lemmens, P.C.; Ruivenkamp, G.T.P.

    2015-01-01

    This article focuses on the power of technological mediation from the point of view of autonomist Marxism (Hardt, Negri, Virno, Berardi, Lazzarrato). The first part of the article discusses the theories developed on technological mediation in postphenomenology (Ihde, Verbeek) and critical theory of

  1. Technology-Mediated Supervision of Undergraduate Students' Dissertations

    Science.gov (United States)

    Jaldemark, Jimmy; Lindberg, J. Ola

    2013-01-01

    In Sweden, technology-mediated participation has increased in tertiary education, which has led to changing conditions for its delivery. However, one part has proven more resistant to change, technology-mediated or not: the supervision of students' undergraduate dissertation work. This article presents a study that analyses technological…

  2. Activation of CFTR by ASBT-mediated bile salt absorption

    NARCIS (Netherlands)

    Bijvelds, MJC; Jorna, H; Verkade, HJ; Bot, AGM; Hofmann, F; Agellon, LB; Sinaasappel, M; de Jonge, HR

    2005-01-01

    In cholangiocytes, bile salt (BS) uptake via the apical sodium-dependent bile acid transporter (ASBT) may evoke ductular flow by enhancing cAMP-mediated signaling to the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel. We considered that ASBT-mediated BS uptake in the distal

  3. Mediating Tourist Experiences. Access to Places via Shared Videos

    DEFF Research Database (Denmark)

    Tussyadiah, Iis; Fesenmaier, D.R.

    2009-01-01

    The emergence of new media using multimedia features has generated a new set of mediators for tourists' experiences. This study examines two hypotheses regarding the roles that online travel videos play as mediators of tourist experiences. The results confirm that online shared videos can provide...

  4. Power Analysis for Complex Mediational Designs Using Monte Carlo Methods

    Science.gov (United States)

    Thoemmes, Felix; MacKinnon, David P.; Reiser, Mark R.

    2010-01-01

    Applied researchers often include mediation effects in applications of advanced methods such as latent variable models and linear growth curve models. Guidance on how to estimate statistical power to detect mediation for these models has not yet been addressed in the literature. We describe a general framework for power analyses for complex…

  5. Mechanisms of rhizobacteria-mediated induced systemic resistance

    NARCIS (Netherlands)

    Hase, S.; Pieterse, C.M.J.; Loon, L.C. van

    2001-01-01

    Some of non-pathogenic rhizosphere bacteria reduce disease by activating a resistance mechanism in the plant called rhizobacteria-mediated induced systemic resistance (ISR). Rhizobacteria-mediated ISR resembles classic pathogen-induced systemic acquired resistance (SAR) in that both types of induced

  6. Mediation in the energy sector : fuel for thought

    Energy Technology Data Exchange (ETDEWEB)

    Trace, K. [McCuaig Desrochers LLP, Edmonton, AB (Canada)

    2006-07-01

    A recent report by the Alberta Energy and Utilities Board (AEUB) has suggested that appropriate dispute resolution processes including mediation are proving to be 85 per cent effective at resolving time-consuming and costly energy disputes. This presentation outlined techniques used in the mediation process. Mediation seeks consensus-based agreement. Unlike arbitration and judicial dispute resolution, mediation assists parties in looking beyond legal rights and issues by exploring the context of the dispute so as to determine the roots of the conflict. Mediation processes are derived from theories of interest-based dispute resolution. In mediation procedures, interests are defined as the underlying hopes, beliefs, needs, and aspirations driving any particular conflict. During the mediation process, mutual interests often emerge as a starting point for consensus-based decision-making. Several case studies were presented in which oil and gas industry members came to satisfactory resolutions with a variety of initially antagonistic stakeholders. A case study on assumptions and three-party mediations was also included. refs., tabs., figs.

  7. 12 CFR 269.9 - Mediation of negotiation impasses.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Mediation of negotiation impasses. 269.9... RESERVE SYSTEM POLICY ON LABOR RELATIONS FOR THE FEDERAL RESERVE BANKS § 269.9 Mediation of negotiation impasses. In the event of an impasse in negotiations between the parties for a collective...

  8. The Use of Communication Strategies in Computer-Mediated Communication.

    Science.gov (United States)

    Smith, Bryan

    2003-01-01

    Examines communication strategy use among adult learners of English in a computer-mediated environment. Specifically explored communication strategies employed during problem-free discourse as well as compensatory strategy use during task-based computer-mediated communication. Strategy use was also examined relative to communicative task type.…

  9. Family Structure and Mediators of Adolescent Drug Use

    Science.gov (United States)

    Broman, Clifford L.; Li, Xin; Reckase, Mark

    2008-01-01

    This study investigates how family structure is associated with adolescent drug use and how parenting, peer use, religiosity, and neighborhood problems may mediate the relationship. The authors use structural equation modeling to examine the relationship between family structure and drug use across race, and examine potential mediators. Using data…

  10. Textbook Mediation of Teaching: An Example from Tertiary Mathematics Instructors

    Science.gov (United States)

    Mesa, Vilma; Griffiths, Brett

    2012-01-01

    Drawing on data from interviews with mathematics faculty in three different types of undergraduate institutions and using Rabardel's model of instrument use (Verillon & Rabardel "1995"), we describe three ways textbooks mediate college faculty work regarding instruction. The model anticipates epistemic and pragmatic mediations between the…

  11. 15 CFR 930.157 - Mediation and informal negotiations.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Mediation and informal negotiations. 930.157 Section 930.157 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade... Activities Having Interstate Coastal Effects § 930.157 Mediation and informal negotiations. The...

  12. A General Multilevel SEM Framework for Assessing Multilevel Mediation

    Science.gov (United States)

    Preacher, Kristopher J.; Zyphur, Michael J.; Zhang, Zhen

    2010-01-01

    Several methods for testing mediation hypotheses with 2-level nested data have been proposed by researchers using a multilevel modeling (MLM) paradigm. However, these MLM approaches do not accommodate mediation pathways with Level-2 outcomes and may produce conflated estimates of between- and within-level components of indirect effects. Moreover,…

  13. Mediators of Yoga and Stretching for Chronic Low Back Pain

    Directory of Open Access Journals (Sweden)

    Karen J. Sherman

    2013-01-01

    Full Text Available Although yoga is an effective treatment for chronic low back pain, little is known about the mechanisms responsible for its benefits. In a trial comparing yoga to intensive stretching and self-care, we explored whether physical (hours of back exercise/week, cognitive (fear avoidance, body awareness, and self-efficacy, affective (psychological distress, perceived stress, positive states of mind, and sleep, and physiological factors (cortisol, DHEA mediated the effects of yoga or stretching on back-related dysfunction (Roland-Morris Disability Scale (RDQ. For yoga, 36% of the effect on 12-week RDQ was mediated by increased self-efficacy, 18% by sleep disturbance, 9% by hours of back exercise, and 61% by the best combination of all possible mediators (6 mediators. For stretching, 23% of the effect was mediated by increased self-efficacy, 14% by days of back exercise, and 50% by the best combination of all possible mediators (7 mediators. In open-ended questions, ≥20% of participants noted the following treatment benefits: learning new exercises (both groups, relaxation, increased awareness, and the benefits of breathing (yoga, benefits of regular practice (stretching. Although both self-efficacy and hours of back exercise were the strongest mediators for each intervention, compared to self-care, qualitative data suggest that they may exert their benefits through partially distinct mechanisms.

  14. An Abstinence Program's Impact on Cognitive Mediators and Sexual Initiation

    Science.gov (United States)

    Weed, Stan E.; Ericksen, Irene H.; Lewis, Allen; Grant, Gale E.; Wibberly, Kathy H.

    2008-01-01

    Objectives: To evaluate the impact of an abstinence education program on sexual intercourse initiation and on possible cognitive mediators of sexual initiation for virgin seventh graders in suburban Virginia. Methods: Measures of sexual behavior and 6 mediating variables were compared at 3 time periods for program participants and a matched…

  15. Counterfactual Graphical Models for Longitudinal Mediation Analysis with Unobserved Confounding

    Science.gov (United States)

    Shpitser, Ilya

    2013-01-01

    Questions concerning mediated causal effects are of great interest in psychology, cognitive science, medicine, social science, public health, and many other disciplines. For instance, about 60% of recent papers published in leading journals in social psychology contain at least one mediation test (Rucker, Preacher, Tormala, & Petty, 2011).…

  16. Mediators of yoga and stretching for chronic low back pain.

    Science.gov (United States)

    Sherman, Karen J; Wellman, Robert D; Cook, Andrea J; Cherkin, Daniel C; Ceballos, Rachel M

    2013-01-01

    Although yoga is an effective treatment for chronic low back pain, little is known about the mechanisms responsible for its benefits. In a trial comparing yoga to intensive stretching and self-care, we explored whether physical (hours of back exercise/week), cognitive (fear avoidance, body awareness, and self-efficacy), affective (psychological distress, perceived stress, positive states of mind, and sleep), and physiological factors (cortisol, DHEA) mediated the effects of yoga or stretching on back-related dysfunction (Roland-Morris Disability Scale (RDQ)). For yoga, 36% of the effect on 12-week RDQ was mediated by increased self-efficacy, 18% by sleep disturbance, 9% by hours of back exercise, and 61% by the best combination of all possible mediators (6 mediators). For stretching, 23% of the effect was mediated by increased self-efficacy, 14% by days of back exercise, and 50% by the best combination of all possible mediators (7 mediators). In open-ended questions, ≥20% of participants noted the following treatment benefits: learning new exercises (both groups), relaxation, increased awareness, and the benefits of breathing (yoga), benefits of regular practice (stretching). Although both self-efficacy and hours of back exercise were the strongest mediators for each intervention, compared to self-care, qualitative data suggest that they may exert their benefits through partially distinct mechanisms.

  17. Values-led Participatory Design - Mediating the Emergence of Values

    DEFF Research Database (Denmark)

    Iversen, Ole Sejer; Leong, Tuck Wah

    2012-01-01

    with severe intellectual disabilities, in an art museum. By describing how we establish, negotiate and the debrief values during this initial phase of a values-led inquiry. By foregrounding both explicit and implicit mediation in the PD process we show how a theoretical understanding of mediation can...

  18. Agrobacterium-mediated transformation of Petunia leaf discs

    NARCIS (Netherlands)

    Meer, van der I.M.

    2006-01-01

    Many dicotyledonous and also several monocotyledonous plant species are susceptible to Agrobacterium-mediated transformation. This current and well-established method has been used successfully with a large number of plant species to mediate gene transfer. This chapter describes an Agrobacterium-med

  19. HUMAN COMMUNICATION AS MEDIATING THE UNITS OF PARAMETERISED ENVIRONMENT

    Directory of Open Access Journals (Sweden)

    Josip Stepanic

    2004-06-01

    Full Text Available Human communication is prevalently a mediated process. Mediators are units of environment, which are attributed functions within the local value set. They are utilised in such a way as to optimise the change of human states. In this article, a mediator-centred interpretation of the human communication is given. The interpretation follows closely the concept of mediated interaction developed within physics. It is conjectured that collection of mediators, which the humans use, has a well-defined average. The averaged collection permits reliable interpretation as a human communication spectrum. Relation of the intensity of a spectral component with regard to different senses, and with regard to intensity of interaction is discussed.

  20. The Dark Side of Computer-Mediated Control

    DEFF Research Database (Denmark)

    Cunha, J. V.; Carugati, Andrea; Leclercq, A.

    2015-01-01

    Research on the dark side of computer-mediated control has explained the consequences of computer-mediated control when work is tightly coupled with its electronic representation because information systems record work automatically. Our study complements prior research by addressing the dark side...... of computer-mediated control when work and its electronic representation are loosely coupled, because it is employees who report their work in IT systems. Data from a 15-month ethnographic study of the appropriation of a customer relationship management system in the sales department of a large organization...... reveal that loosely versus tightly coupled computer-mediated control produce key differences that re-specify the dark side of computer-mediated control....

  1. Strongly Coupled Semi-Direct Mediation of Supersymmetry Breaking

    Energy Technology Data Exchange (ETDEWEB)

    Ibe, M.; /SLAC; Izawa, K.-I.; /Kyoto U., Yukawa Inst., Kyoto /Tokyo U., IPMU; Nakai, Y.; /Kyoto U., Yukawa Inst., Kyoto

    2011-09-13

    Supersymmetry (SUSY) is expected to be a crucial ingredient of basic laws in Nature. It is an attractive possibility that SUSY is broken at low energy within the experimental reach in the near future. Among others, low-energy dynamics with gauge mediation between a hidden sector of SUSY breaking and the visible sector of SUSY standard model may be phenomenologically viable. In particular, the gauge interactions are flavor blind, so that the unwanted flavor-changing processes are naturally suppressed. Strongly coupled semi-direct gauge mediation models of supersymmetry breaking through massive mediators with standard model charges are investigated by means of composite degrees of freedom. Sizable mediation is realized to generate the standard model gaugino masses for a small mediator mass without breaking the standard model symmetries.

  2. The Mediatization of Politics in Contemporary Scandinavian Film and Television

    DEFF Research Database (Denmark)

    Bondebjerg, Ib

    2015-01-01

    The increased mediatization of society since 1960 has lead to a discussion of the mediatization of politics and the role of media for political debate and democracy. Habermas as early as 1962 discussed the rise and potential decline of the public sphere. The role of media in general was central...... in this debate, and the rise of the internet in modern societies has increased the analysis and theories of the mediatization of politics in theories of democracy and in media sociology. Audio–visual media and the new digital media have increased the visibility of all parts of private and public life and changed...... the relation between media, public figures and the citizen. In this article, I will discuss main positions in theories on the mediatization of politics. I will also discuss and analyse three types of film and television genres that reflect this mediatization of politics in Scandinavian film and television...

  3. Exosome mediated communication within the tumor microenvironment.

    Science.gov (United States)

    Milane, Lara; Singh, Amit; Mattheolabakis, George; Suresh, Megha; Amiji, Mansoor M

    2015-12-10

    It is clear that exosomes (endosome derived vesicles) serve important roles in cellular communication both locally and distally and that the exosomal process is abnormal in cancer. Cancer cells are not malicious cells; they are cells that represent 'survival of the fittest' at its finest. All of the mutations, abnormalities, and phenomenal adaptations to a hostile microenvironment, such as hypoxia and nutrient depletion, represent the astute ability of cancer cells to adapt to their environment and to intracellular changes to achieve a single goal - survival. The aberrant exosomal process in cancer represents yet another adaptation that promotes survival of cancer. Cancer cells can secrete more exosomes than healthy cells, but more importantly, the content of cancer cells is distinct. An illustrative distinction is that exosomes derived from cancer cells contain more microRNA than healthy cells and unlike exosomes released from healthy cells, this microRNA can be associated with the RNA-induced silencing complex (RISC) which is required for processing mature and biologically active microRNA. Cancer derived exosomes have the ability to transfer metastatic potential to a recipient cell and cancer exosomes function in the physical process of invasion. In this review we conceptualize the aberrant exosomal process (formation, content selection, loading, trafficking, and release) in cancer as being partially attributed to cancer specific differences in the endocytotic process of receptor recycling/degradation and plasma membrane remodeling and the function of the endosome as a signaling entity. We discuss this concept and, to advance comprehension of exosomal function in cancer as mediators of communication, we detail and discuss exosome biology, formation, and communication in health and cancer; exosomal content in cancer; exosomal biomarkers in cancer; exosome mediated communication in cancer metastasis, drug resistance, and interfacing with the immune system; and

  4. Antibody-Mediated Rejection: A Review

    Science.gov (United States)

    Garces, Jorge Carlos; Giusti, Sixto; Staffeld-Coit, Catherine; Bohorquez, Humberto; Cohen, Ari J.; Loss, George E.

    2017-01-01

    Background: Chronic antibody injury is a serious threat to allograft outcomes and is therefore the center of active research. In the continuum of allograft rejection, the development of antibodies plays a critical role. In recent years, an increased recognition of molecular and histologic changes has provided a better understanding of antibody-mediated rejection (AMR), as well as potential therapeutic interventions. However, several pathways are still unknown, which accounts for the lack of efficacy of some of the currently available agents that are used to treat rejection. Methods: We review the current diagnostic criteria for AMR; AMR paradigms; and desensitization, treatment, and prevention strategies. Results: Chronic antibody-mediated endothelial injury results in transplant glomerulopathy, manifested as glomerular basement membrane duplication, double contouring, or splitting. Clinical manifestations of AMR include proteinuria and a rise in serum creatinine. Current strategies for the treatment of AMR include antibody depletion with plasmapheresis (PLEX), immunoadsorption (IA), immunomodulation with intravenous immunoglobulin (IVIG), and T cell– or B cell–depleting agents. Some treatment benefits have been found in using PLEX and IA, and some small nonrandomized trials have identified some benefits in using rituximab and the proteasome inhibitor-based therapy bortezomib. More recent histologic follow-ups of patients treated with bortezomib have not shown significant benefits in terms of allograft outcomes. Furthermore, no specific treatment approaches have been approved by the US Food and Drug Administration. Other agents used for more difficult rejections include bortezomib and eculizumab (an anti-C5 monoclonal antibody). Conclusion: AMR is a fascinating field with ample opportunities for research and progress in the future. Despite the use of advanced techniques for the detection of human leukocyte antigen (HLA) or non-HLA donor-specific antibodies

  5. AGROBACTERIUM-MEDIATED TRANSFORMATION OF WHEAT

    Directory of Open Access Journals (Sweden)

    K. Mészáros

    2008-09-01

    Full Text Available Transformation of cereals is one of the emerging areas for plant genomic and biotechnology research. Wheat was among the last major crops to be transformed by particle bombardment about 10 years ago. However, Agrobacterium-mediated transformation has several advantages over bombardment, including a reduction in copy number, fewer rearrangements and preferential integration into transcriptionally active chromosome regions. As a first step, we started to adapt an immature embryo-based transformation method for the model variety ‘Cadenza’. The regeneration of this variety was low and especially the cost of generating donor plants was high. Therefore, we decided (i to test regeneration capacity of winter and spring wheats using four different explants, (ii to determine the optimal genotype-regeneration system combinations, and (iii to work out the details of mature embryo transformation with Agrobacterium. The experiment was carried out with 16 cultivated winter wheat and 2 model spring wheat varieties. Four different explants: anther, immature embryo, mature embryo and dry seed were tested for callus induction and plant regeneration. The regeneration capacity was the lowest in the case of anther culture and ranged from 20% (‘Mv Béres’ to 0.1% (‘Mv Magvas’ with four varieties exerting significantly higher regeneration than ‘Cadenza’. Plant regeneration from immature embryos ranged between 59% (‘Mv Regiment’ and 0.1% (‘Mv Toborzó’. Again, four varieties produced significantly more plants than the control ‘Cadenza’. We tested two systems for the plant regeneration from mature embryos. First, mature embryos were isolated from seeds, which resulted in an average of 17% plant regeneration (from 63% in ‘Fatima’ to zero in ‘Mv Palotás’. ‘Cadenza’ was one of the worse regenerating genotype (7%. The highest plant regeneration (average 54% was in the case of seed explants. There were no significant differences

  6. Revisiting Mediation in the Social and Behavioral Sciences

    Directory of Open Access Journals (Sweden)

    Aurelio José Figueredo

    2013-11-01

    Full Text Available The process of mediation is of critical importance to the social and behavioral sciences and to evolutionary social psychology in particular. As with the concept of evolutionary adaptation, however, one can argue that causal mediation is in need of explicit theoretical justification and empirical support. Mainstream evolutionary social psychology proposes, for example, that organisms are “adaptation executers”, and not “fitness maximizers”. The execution of adaptations is triggered by fitness-relevant ecological contingencies at both ultimate and proximate levels of analysis. This logic is essentially equivalent to what methodologists refer to as the process of mediation; the adaptations to be executed (or not, depending upon the prevailing environmental circumstances causally mediate the effects of the ecological contingencies upon the fitness outcomes. Thus, the process of mediation can be generally conceptualized as a causal chain of events leading to a given outcome or set of outcomes. If a predictor variable operates through an intervening variable to affect a criterion variable, then mediation is said to exist. Nevertheless, it does not appear that some psychologists (particularly evolutionary-social psychologists are sufficiently well-versed in the fundamental logic and quantitative methodology of establishing causal mediation to support such claims. In the current paper, we set out to review the ways researchers support their use of mediation statements and also propose critical considerations on this front. We start with more conventional methods for testing mediation, discuss variants of the conventional approach, discuss the limitations of such methods as we see them, and end with our preferred mediation approach. DOI: 10.2458/azu_jmmss.v4i1.17761

  7. Standardized Effect Size Measures for Mediation Analysis in Cluster-Randomized Trials

    Science.gov (United States)

    Stapleton, Laura M.; Pituch, Keenan A.; Dion, Eric

    2015-01-01

    This article presents 3 standardized effect size measures to use when sharing results of an analysis of mediation of treatment effects for cluster-randomized trials. The authors discuss 3 examples of mediation analysis (upper-level mediation, cross-level mediation, and cross-level mediation with a contextual effect) with demonstration of the…

  8. The Effects of Peer Mediation with Young Children (PMYC) on Children's Cognitive Modifiability

    Science.gov (United States)

    Tzuriel, David; Shamir, Adina

    2007-01-01

    Peer mediation with young children is a relatively novel approach aimed at teaching young children how to mediate to their peers. The main benefits of peer mediation are in developing children's mediation teaching style and cognitive modifiability. The peer mediation developed recently is based on Vygotsky's sociocultural and Feuerstein's…

  9. Kinins as mediators of intestinal secretion.

    Science.gov (United States)

    Gaginella, T S; Kachur, J F

    1989-01-01

    Kinins are small peptides that have diverse biological actions. Concentrations of kinins in the nanomolar or subnanomolar range induce intestinal smooth muscle contraction and evoke mucosal electrolyte secretion. Hyperkininemia is associated with effects on gastrointestinal motility and intestinal mucosal inflammation. Bradykinin and kallidin are the predominant kinins with effects on the gastrointestinal tract of mammals. Bradykinin stimulates chloride ion secretion by the guinea pig and rabbit ileum, rabbit colon, rat colon and monolayers of human HCA-7 cells. Kinins directly or indirectly stimulate phospholipase A2 and phospholipase C. Cells in the lamina propria of the mucosa (e.g., fibroblasts, mast cells, leukocytes), by liberating cyclooxygenase and lipoxygenase metabolites of arachidonic acid, are involved in the kinin response; direct effects on epithelial cells cannot be ruled out, however. Antagonists now exist for kinin receptors. Based on studies with these antagonists in smooth muscle preparations, two subgroups of kinin receptor have been identified. The B2-type receptor appears to be responsible for both the contraction of ileal muscle and ileal secretion. Kinins are probably more important as pathophysiological rather than as physiological mediators. They may amplify the effect of inflammatory products that induce intestinal secretion. The precise involvement of kinins in clinical mucosal secretory states and diarrhea will require quantitative assessment of their levels during each phase of mucosal inflammation. Additional studies on the mechanism of action of kinins will be essential in designing therapy to mitigate the symptoms associated with mucosal inflammation.

  10. Mediation as a source of social development

    Directory of Open Access Journals (Sweden)

    Alpern Ljudmila

    2006-01-01

    Full Text Available In this paper, the author deals with prison as an archaic social institution, which reflects an archaic conception of human being, his needs and duty, but exists in a modern society. Russian prisons are institutions of a male initiations as well as a Russian army. They give a special sort of male socialization, very archaic and military, patriarchal and hierarchal; produce a special kind of society divided on unmixed social groups, casts, and is very violent. Taking into account how many people go through prison in Russia (rotation near 300 people per year, every 4th man got in contact with prison and every 3rd with army and a fact that prison and society are communicating vessels, our prisons, our prisoners and former prisoners are a good reserve of our social underdevelopment, social cruelty, our disability to promote social reforms, to take care about vulnerable group of our population (children, old people and female, because they are not a part of male prison and a military hierarchy. An attempt to modernize prison life, prison condition by different way and especially mediation as a way to make them softer and human is an attempt of social development.

  11. Antibody-mediated resistance against plant pathogens.

    Science.gov (United States)

    Safarnejad, Mohammad Reza; Jouzani, Gholamreza Salehi; Tabatabaei, Meisam; Tabatabaie, Meisam; Twyman, Richard M; Schillberg, Stefan

    2011-01-01

    Plant diseases have a significant impact on the yield and quality of crops. Many strategies have been developed to combat plant diseases, including the transfer of resistance genes to crops by conventional breeding. However, resistance genes can only be introgressed from sexually-compatible species, so breeders need alternative measures to introduce resistance traits from more distant sources. In this context, genetic engineering provides an opportunity to exploit diverse and novel forms of resistance, e.g. the use of recombinant antibodies targeting plant pathogens. Native antibodies, as a part of the vertebrate adaptive immune system, can bind to foreign antigens and eliminate them from the body. The ectopic expression of antibodies in plants can also interfere with pathogen activity to confer disease resistance. With sufficient knowledge of the pathogen life cycle, it is possible to counter any disease by designing expression constructs so that pathogen-specific antibodies accumulate at high levels in appropriate sub-cellular compartments. Although first developed to tackle plant viruses and still used predominantly for this purpose, antibodies have been targeted against a diverse range of pathogens as well as proteins involved in plant-pathogen interactions. Here we comprehensively review the development and implementation of antibody-mediated disease resistance in plants.

  12. Electron attraction mediated by Coulomb repulsion

    Science.gov (United States)

    Hamo, A.; Benyamini, A.; Shapir, I.; Khivrich, I.; Waissman, J.; Kaasbjerg, K.; Oreg, Y.; von Oppen, F.; Ilani, S.

    2016-07-01

    One of the defining properties of electrons is their mutual Coulomb repulsion. However, in solids this basic property may change; for example, in superconductors, the coupling of electrons to lattice vibrations makes the electrons attract one another, leading to the formation of bound pairs. Fifty years ago it was proposed that electrons can be made attractive even when all of the degrees of freedom in the solid are electronic, by exploiting their repulsion from other electrons. This attraction mechanism, termed ‘excitonic’, promised to achieve stronger and more exotic superconductivity. Yet, despite an extensive search, experimental evidence for excitonic attraction has yet to be found. Here we demonstrate this attraction by constructing, from the bottom up, the fundamental building block of the excitonic mechanism. Our experiments are based on quantum devices made from pristine carbon nanotubes, combined with cryogenic precision manipulation. Using this platform, we demonstrate that two electrons can be made to attract each other using an independent electronic system as the ‘glue’ that mediates attraction. Owing to its tunability, our system offers insights into the underlying physics, such as the dependence of the emergent attraction on the underlying repulsion, and the origin of the pairing energy. We also demonstrate transport signatures of excitonic pairing. This experimental demonstration of excitonic pairing paves the way for the design of exotic states of matter.

  13. Pathogenesis of spinally mediated hyperalgesia in diabetes.

    Science.gov (United States)

    Ramos, Khara M; Jiang, Yun; Svensson, Camilla I; Calcutt, Nigel A

    2007-06-01

    Hyperalgesia to noxious stimuli is accompanied by increased spinal cyclooxygenase (COX)-2 protein in diabetic rats. The present studies were initiated to establish causality between increased spinal COX-2 activity and hyperalgesia during diabetes and to assess the potential involvement of polyol pathway activity in the pathogenesis of spinally mediated hyperalgesia. Rats with 1, 2, or 4 weeks of streptozotocin-induced diabetes exhibited significantly increased levels of spinal COX-2 protein and activity, along with exaggerated paw flinching in response to 0.5% paw formalin injection. Increased flinching of diabetic rats was attenuated by intrathecal pretreatment with a selective COX-2 inhibitor immediately before formalin injection, confirming the involvement of COX-2 activity in diabetic hyperalgesia. Chronic treatment with insulin or ICI222155, an aldose reductase inhibitor (ARI) previously shown to prevent spinal polyol accumulation and formalin-evoked hyperalgesia in diabetic rats, prevented elevated spinal COX-2 protein and activity in diabetic rats. In contrast, the ARI IDD676 had no effect on spinal polyol accumulation, elevated spinal COX-2, or hyperalgesia to paw formalin injection. In the spinal cord, aldose reductase immunoreactivity was present solely in oligodendrocytes, which also contained COX-2 immunoreactivity. Polyol pathway flux in spinal oligodendrocytes provides a pathogenic mechanism linking hyperglycemia to hyperalgesia in diabetic rats.

  14. Pathogenesis of immune-mediated neuropathies.

    Science.gov (United States)

    Dalakas, Marinos C

    2015-04-01

    Autoimmune neuropathies occur when immunologic tolerance to myelin or axonal antigens is lost. Even though the triggering factors and the underling immunopathology have not been fully elucidated in all neuropathy subsets, immunological studies on the patients' nerves, transfer experiments with the patients' serum or intraneural injections, and molecular fingerprinting on circulating autoantibodies or autoreactive T cells, indicate that cellular and humoral factors, either independently or in concert with each other, play a fundamental role in their cause. The review is focused on the main subtypes of autoimmune neuropathies, mainly the Guillain-Barré syndrome(s), the Chronic Inflammatory Demyelinating Polyneuropathy (CIDP), the Multifocal Motor Neuropathy (MMN), and the IgM anti-MAG-antibody mediated neuropathy. It addresses the factors associated with breaking tolerance, examines the T cell activation process including co-stimulatory molecules and key cytokines, and discusses the role of antibodies against peripheral nerve glycolipids or glycoproteins. Special attention is given to the newly identified proteins in the nodal, paranodal and juxtaparanodal regions as potential antigenic targets that could best explain conduction failure and rapid recovery. New biological agents against T cells, cytokines, B cells, transmigration and transduction molecules involved in their immunopathologic network, are discussed as future therapeutic options in difficult cases. This article is part of a Special Issue entitled: Neuromuscular Diseases: Pathology and Molecular Pathogenesis.

  15. Protein-mediated surface structuring in biomembranes

    Directory of Open Access Journals (Sweden)

    Maggio B.

    2005-01-01

    Full Text Available The lipids and proteins of biomembranes exhibit highly dissimilar conformations, geometrical shapes, amphipathicity, and thermodynamic properties which constrain their two-dimensional molecular packing, electrostatics, and interaction preferences. This causes inevitable development of large local tensions that frequently relax into phase or compositional immiscibility along lateral and transverse planes of the membrane. On the other hand, these effects constitute the very codes that mediate molecular and structural changes determining and controlling the possibilities for enzymatic activity, apposition and recombination in biomembranes. The presence of proteins constitutes a major perturbing factor for the membrane sculpturing both in terms of its surface topography and dynamics. We will focus on some results from our group within this context and summarize some recent evidence for the active involvement of extrinsic (myelin basic protein, integral (Folch-Lees proteolipid protein and amphitropic (c-Fos and c-Jun proteins, as well as a membrane-active amphitropic phosphohydrolytic enzyme (neutral sphingomyelinase, in the process of lateral segregation and dynamics of phase domains, sculpturing of the surface topography, and the bi-directional modulation of the membrane biochemical reactivity.

  16. Collisionless shock waves mediated by Weibel Instability

    Science.gov (United States)

    Naseri, Neda; Ruan, Panpan; Zhang, Xi; Khudik, Vladimir; Shvets, Gennady

    2015-11-01

    Relativistic collisionless shocks are common events in astrophysical environments. They are thought to be responsible for generating ultra-high energy particles via the Fermi acceleration mechanism. It has been conjectured that the formation of collisionless shocks is mediated by the Weibel instability that takes place when two initially cold, unmagnetized plasma shells counter-propagate into each other with relativistic drift velocities. Using a PIC code, VLPL, which is modified to suppress numerical Cherenkov instabilities, we study the shock formation and evolution for asymmetric colliding shells with different densities in their own proper reference frame. Plasma instabilities in the region between the shock and the precursor are also investigated using a moving-window simulation that advances the computational domain at the shock's speed. This method helps both to save computation time and avoid severe numerical Cherenkov instabilities, and it allows us to study the shock evolution in a longer time period. Project is supported by US DOE grants DE-FG02-04ER41321 and DE-FG02-07ER54945.

  17. Insulin signaling mediates sexual attractiveness in Drosophila.

    Directory of Open Access Journals (Sweden)

    Tsung-Han Kuo

    Full Text Available Sexually attractive characteristics are often thought to reflect an individual's condition or reproductive potential, but the underlying molecular mechanisms through which they do so are generally unknown. Insulin/insulin-like growth factor signaling (IIS is known to modulate aging, reproduction, and stress resistance in several species and to contribute to variability of these traits in natural populations. Here we show that IIS determines sexual attractiveness in Drosophila through transcriptional regulation of genes involved in the production of cuticular hydrocarbons (CHC, many of which function as pheromones. Using traditional gas chromatography/mass spectrometry (GC/MS together with newly introduced laser desorption/ionization orthogonal time-of-flight mass spectrometry (LDI-MS we establish that CHC profiles are significantly affected by genetic manipulations that target IIS. Manipulations that reduce IIS also reduce attractiveness, while females with increased IIS are significantly more attractive than wild-type animals. IIS effects on attractiveness are mediated by changes in CHC profiles. Insulin signaling influences CHC through pathways that are likely independent of dFOXO and that may involve the nutrient-sensing Target of Rapamycin (TOR pathway. These results suggest that the activity of conserved molecular regulators of longevity and reproductive output may manifest in different species as external characteristics that are perceived as honest indicators of fitness potential.

  18. Fibroblasts in fibrosis: novel roles and mediators

    Directory of Open Access Journals (Sweden)

    Ryan Thomas Kendall

    2014-05-01

    Full Text Available Fibroblasts are the most common cell type of the connective tissues found throughout the body and the principal source of the extensive extracellular matrix (ECM characteristic of these tissues. They are also the central mediators of the pathological fibrotic accumulation of ECM and the cellular proliferation and differentiation that occurs in response to prolonged tissue injury and chronic inflammation. The transformation of the fibroblast cell lineage involves classical developmental signaling programs and includes a surprisingly diverse range of precursor cell types—most notably, myofibroblasts that are the apex of the fibrotic phenotype. Myofibroblasts display exaggerated ECM production; constitutively secrete and are hypersensitive to chemical signals such as cytokines, chemokines, and growth factors; and are endowed with a contractile apparatus allowing them to manipulate the ECM fibers physically to close open wounds. In addition to ECM production, fibroblasts have multiple concomitant biological roles, such as in wound healing, inflammation, and angiogenesis, which are each interwoven with the process of fibrosis. We now recognize many common fibroblast-related features across various physiological and pathological protracted processes. Indeed, a new appreciation has emerged for the role of noncancerous fibroblast interactions with tumors in cancer progression. Although the predominant current clinical treatments of fibrosis involve nonspecific immunosuppressive and anti-proliferative drugs, a variety of potential therapies under investigation specifically target fibroblast biology.

  19. Anandamide mediates cognitive judgement bias in rats.

    Science.gov (United States)

    Kregiel, J; Malek, N; Popik, P; Starowicz, K; Rygula, R

    2016-02-01

    In the present study, we investigated the effects of acute pharmacological manipulation of the endocannabinoid (EC) system on the valence of cognitive judgement bias of rats in the ambiguous-cue interpretation (ACI) paradigm. To accomplish this goal, after initial behavioural training, different groups of rats received single, systemic injections of the irreversible anandamide (AEA) hydrolysis inhibitor URB597, the cannabinoid receptor type 1 (CB1) inverse agonist AM251, the cannabinoid receptor type 2 (CB2) inverse agonist AM630, the combination of URB597 and AM251, and a combination of URB597 and AM630 and were subsequently tested with the ACI paradigm. We report that URB597 at a dose of 1 mg/kg significantly biased animals towards positive interpretation of the ambiguous cue and that this effect was abolished by pre-treatment with AM251 (1 mg/kg) or AM630 (1 mg/kg). The CB1 and CB2 inverse agonists administered alone (1 mg/kg) had no statistically significant effects on the interpretation of the ambiguous cue by rats. Our findings suggest involvement of the endocannabinoid system in the mediation of optimistic judgement bias.

  20. Exchange bias mediated by interfacial nanoparticles (invited)

    Energy Technology Data Exchange (ETDEWEB)

    Berkowitz, A. E., E-mail: aberk@ucsd.edu [Department of Physics, University of California, San Diego, La Jolla, California 92093 (United States); Center for Magnetic Recording Research, University of California, California 92093 (United States); Sinha, S. K. [Department of Physics, University of California, San Diego, La Jolla, California 92093 (United States); Fullerton, E. E. [Center for Magnetic Recording Research, University of California, California 92093 (United States); Smith, D. J. [Department of Physics, Arizona State University, Tempe, Arizona 85287 (United States)

    2015-05-07

    The objective of this study on the iconic exchange-bias bilayer Permalloy/CoO has been to identify those elements of the interfacial microstructure and accompanying magnetic properties that are responsible for the exchange-bias and hysteretic properties of this bilayer. Both epitaxial and polycrystalline samples were examined. X-ray and neutron reflectometry established that there existed an interfacial region, of width ∼1 nm, whose magnetic properties differed from those of Py or CoO. A model was developed for the interfacial microstructure that predicts all the relevant properties of this system; namely; the temperature and Permalloy thickness dependence of the exchange-bias, H{sub EX}, and coercivity, H{sub C}; the much smaller measured values of H{sub EX} from what was nominally expected; the different behavior of H{sub EX} and H{sub C} in epitaxial and polycrystalline bilayers. A surprising result is that the exchange-bias does not involve direct exchange-coupling between Permalloy and CoO, but rather is mediated by CoFe{sub 2}O{sub 4} nanoparticles in the interfacial region.

  1. A redox-mediated Kemp eliminase

    Science.gov (United States)

    Li, Aitao; Wang, Binju; Ilie, Adriana; Dubey, Kshatresh D.; Bange, Gert; Korendovych, Ivan V.; Shaik, Sason; Reetz, Manfred T.

    2017-01-01

    The acid/base-catalysed Kemp elimination of 5-nitro-benzisoxazole forming 2-cyano-4-nitrophenol has long served as a design platform of enzymes with non-natural reactions, providing new mechanistic insights in protein science. Here we describe an alternative concept based on redox catalysis by P450-BM3, leading to the same Kemp product via a fundamentally different mechanism. QM/MM computations show that it involves coordination of the substrate's N-atom to haem-Fe(II) with electron transfer and concomitant N–O heterolysis liberating an intermediate having a nitrogen radical moiety Fe(III)–N· and a phenoxyl anion. Product formation occurs by bond rotation and H-transfer. Two rationally chosen point mutations cause a notable increase in activity. The results shed light on the prevailing mechanistic uncertainties in human P450-catalysed metabolism of the immunomodulatory drug leflunomide, which likewise undergoes redox-mediated Kemp elimination by P450-BM3. Other isoxazole-based pharmaceuticals are probably also metabolized by a redox mechanism. Our work provides a basis for designing future artificial enzymes. PMID:28348375

  2. Vitreous Mediators in Retinal Hypoxic Diseases

    Directory of Open Access Journals (Sweden)

    Roberto dell'Omo

    2013-01-01

    Full Text Available The causes of retinal hypoxia are many and varied. Under hypoxic conditions, a variety of soluble factors are secreted into the vitreous cavity including growth factors, cytokines, and chemokines. Cytokines, which usually serve as signals between neighboring cells, are involved in essentially every important biological process, including cell proliferation, inflammation, immunity, migration, fibrosis, tissue repair, and angiogenesis. Cytokines and chemokines are multifunctional mediators that can direct the recruitment of leukocytes to sites of inflammation, promote the process, enhance immune responses, and promote stem cell survival, development, and homeostasis. The modern particle-based flow cytometric analysis is more direct, stable and sensitive than the colorimetric readout of the conventional ELISA but, similar to ELISA, is influenced by vitreous hemorrhage, disruption of the blood-retina barrier, and high serum levels of a specific protein. Finding patterns in the expression of inflammatory cytokines specific to a particular disease can substantially contribute to the understanding of its basic mechanism and to the development of a targeted therapy.

  3. Vocally mediated social recognition in anurans

    Science.gov (United States)

    Bee, Mark A.

    2005-09-01

    Anuran amphibians (frogs and toads) are among the most vocal of vertebrates and have long served as model systems for investigating the mechanisms and evolution of acoustic communication. Compared to higher vertebrates, however, the role of cognition in anuran communication has received less attention, at least in part due to the lack of evidence that juvenile anurans learn to produce signals or associate them with particular social contexts. Recent studies of social recognition in two anuran families indicate that territorial male frogs in some species are able to learn about and recognize the individually distinctive properties of the calls of nearby neighbors. For example, male bullfrogs (ranidae) learn about the pitch of a neighbor's vocalizations (an individually distinct voice property) and associate a familiar pitch with the location of the neighbor's territory. As in songbirds, this form of vocally mediated social recognition allows territory holders to direct low levels of aggression toward well-established neighbors, while maintaining a readiness to respond aggressively to more threatening strangers that may attempt a territory takeover. A brief review of currently available data will be used to illustrate how anurans can serve as model systems for investigating the role of cognition in acoustic communication.

  4. Statins attenuate polymethylmethacrylate-mediated monocyte activation.

    LENUS (Irish Health Repository)

    Laing, Alan J

    2012-02-03

    BACKGROUND: Periprosthetic osteolysis precipitates aseptic loosening of components, increases the risk of periprosthetic fracture and, through massive bone loss, complicates revision surgery and ultimately is the primary cause for failure of joint arthroplasty. The anti-inflammatory properties of HMG-CoA reductase inhibitors belonging to the statin family are well recognized. We investigated a possible role for status in initiating the first stage of the osteolytic cycle, namely monocytic activation. METHODS: We used an in vitro model of the human monocyte\\/macrophage inflammatory response to poly-methylmethacrylate (PMMA) particles after pretreat-ing cells with cerivastatin, a potent member of the statin family. Cell activation based upon production of TNF-alpha and MCP-1 cytokines was analyzed and the intracellular Raf-MEK-ERK signal transduction pathway was evaluated using western blot analysis, to identify its role in cell activation and in any cerivastatin effects observed. RESULTS: We found that pretreatment with cerivastatin significantly abrogates the production of inflammatory cytokines TNF-alpha and MCP-1 by human monocytes in response to polymethylmethacrylate particle activation. This inflammatory activation and attenuation appear to be mediated through the intracellular Raf-MEK-ERK pathway. INTERPRETATION: We propose that by intervening at the upstream activation stage, subsequent osteoclast activation and osteolysis can be suppressed. We believe that the anti-inflammatory properties of statins may potentially play a prophylactic role in the setting of aseptic loosening, and in so doing increase implant longevity.

  5. Dynamics of target-mediated drug disposition.

    Science.gov (United States)

    Peletier, Lambertus A; Gabrielsson, Johan

    2009-12-08

    We present a mathematical analysis of the basic model underlying target-mediated drug disposition (TMDD) in which a ligand is supplied through an initial bolus or through a constant rate infusion and forms a complex with a receptor (target), which is supplied and removed continuously. Ligand and complex may be eliminated according to first-order processes. We assume that the total receptor pool (free and bound) is constant in time and we give a geometrical description of the evolution of the concentrations of ligand, receptor and receptor-ligand complex which offers a transparent way to compare the full model with simpler models such as the quasi-steady-state (QSS) model, the quasi-equilibrium (QE) model and the empirical Michaelis-Menten (MM) model; we also give precise conditions on the parameters in the TMDD model for the validity of these reduced models. We relate characteristic properties of time courses to parameter regimes and, in particular, we identify and explain non-monotone dependence of the time-to-steady-state on the infusion rate. Finally, we discuss how the volume of the central compartment may be overestimated because of singular initial behaviour of the time course of the ligand concentration.

  6. Information Systems as Dialectic, Tool - Mediated Activity

    Directory of Open Access Journals (Sweden)

    Helen Hasan

    2002-11-01

    Full Text Available Information Systems (IS draws its significance from the uniqueness of computer-based information and communications tools and their place in shaping recent human history. Advances in the field come from a better understanding of how to develop and use these tools and what impact they have on the way we work, and live. As IS is still an evolving field of study, two views, the objective and the subjective, are in constant tension and, though these may be considered complementary, it is rare that they come together as a unified whole. A more balanced, and integrated, foundation for IS may be found in the subject-object dialectic arising out of the German philosophical tradition. An extension of this approach from the Cultural-Historical Activity Theory is presented in this paper. This theory views all human endeavour as a purposeful, dynamic, dialectic relationship between subject and object, mediated by tools, such as technology and information, and by the social environment or community. An adaptation of this holistic theory, to incorporate the best of other theoretical approaches used by IS researchers, could span and integrate the breadth of the field IS providing it with unity and identity.

  7. Nrf2 mediates redox adaptations to exercise

    Directory of Open Access Journals (Sweden)

    Aaron J. Done

    2016-12-01

    Full Text Available The primary aim of this review is to summarize the current literature on the effects of acute exercise and regular exercise on nuclear factor erythroid 2-related factor 2 (Nrf2 activity and downstream targets of Nrf2 signaling. Nrf2 (encoded in humans by the NFE2L2 gene is the master regulator of antioxidant defenses, a transcription factor that regulates expression of more than 200 cytoprotective genes. Increasing evidence indicates that Nrf2 signaling plays a key role in how oxidative stress mediates the beneficial effects of exercise. Episodic increases in oxidative stress induced through bouts of acute exercise stimulate Nrf2 activation and when applied repeatedly, as with regular exercise, leads to upregulation of endogenous antioxidant defenses and overall greater ability to counteract the damaging effects of oxidative stress. The evidence of Nrf2 activation in response to exercise across variety of tissues may be an important mechanism of how exercise exerts its well-known systemic effects that are not limited to skeletal muscle and myocardium. Additionally there are emerging data that results from animal studies translate to humans.

  8. Less is More when Gluinos Mediate

    CERN Document Server

    Nachman, Benjamin

    2015-01-01

    Compressed mass spectra are generally more difficult to identify than spectra with large splittings. In particular, gluino pair production with four high energy top or bottom quarks leaves a striking signature in a detector. However, if any of the mass splittings are compressed, the power of traditional techniques may deteriorate. Searches for direct stop/sbottom pair production can fill in the gaps. As a demonstration, we show that for $\\tilde{g}\\rightarrow t\\tilde{t}_1$ and $m_{\\tilde{t}_1}\\sim m_{\\tilde{\\chi}_1^0}$, limits on the stop mass at 8 TeV can be extended by least 300 GeV for a 1.1 TeV gluino using a $pp\\rightarrow \\tilde{t}_1\\tilde{t}_1$ search. At 13 TeV, the effective cross section for the gluino mediated process is twice the direct stop/sbottom pair production cross section, suggesting that direct stop/sbottom searches could be sensitive to discover new physics earlier than expected.

  9. Non-universalities in pure gravity mediation

    Energy Technology Data Exchange (ETDEWEB)

    Evans, Jason L.; Olive, Keith A. [University of Minnesota, William I. Fine Theoretical Physics Institute, School of Physics and Astronomy, Minneapolis, MN (United States); Ibe, Masahiro [University of Tokyo, ICRR, Kashiwa (Japan); University of Tokyo, Kavli IPMU (WPI), TODIAS, Kashiwa (Japan); Yanagida, Tsutomu T. [University of Tokyo, Kavli IPMU (WPI), TODIAS, Kashiwa (Japan)

    2013-10-15

    The simplest model of pure gravity mediation contains only two free parameters: the gravitino mass and tan{beta}. Scalar masses are universal at some high energy renormalization scale and gaugino masses are determined through anomalies and depend on the gravitino mass and the gauge couplings. This theory requires a relatively large gravitino mass (m{sub 3/2}>or similar 300 TeV) and a limited range in tan{beta}{approx_equal}1.7-2.5. Here we generalize the theory to allow for non-universality in the Higgs soft masses. This introduces zero, one or two new free parameters associated with Higgs soft masses. This generalization allows us to greatly increase the allowed range in tan{beta} and it allows one to find viable solutions with lower m{sub 3/2}. The latter is important if we hope to find a low energy signal from gluinos. Some special cases of these non-universalities are suggestive of Higgs bosons as Nambu-Goldstone bosons or a partial no-scale structure for the Higgs doublets. Thus, we probe signatures at the weak scale and structures at the GUT and/or Planck scale. (orig.)

  10. Lepton flavor violation in flavored gauge mediation

    Energy Technology Data Exchange (ETDEWEB)

    Calibbi, Lorenzo [Universite Libre de Bruxelles, Service de Physique Theorique, Brussels (Belgium); Paradisi, Paride [Universita di Padova, Dipartimento di Fisica e Astronomia, Padua (Italy); INFN Sezione di Padova, Padua (Italy); SISSA, Trieste (Italy); Ziegler, Robert [Sorbonne Universites, UPMC Univ Paris 06, UMR 7589, LPTHE, Paris (France); CNRS, UMR 7589, LPTHE, Paris (France)

    2014-12-01

    We study the anatomy and phenomenology of lepton flavor violation (LFV) in the context of flavored gauge mediation (FGM). Within FGM, the messenger sector couples directly to the MSSM matter fields with couplings controlled by the same dynamics that explains the hierarchies in the SM Yukawas. Although the pattern of flavor violation depends on the particular underlying flavor model, FGM provides a built-in flavor suppression similar to wave function renormalization or SUSY partial compositeness. Moreover, in contrast to these models, there is an additional suppression of left-right flavor transitions by third-generation Yukawas that in particular provides an extra protection against flavor-blind phases. We exploit the consequences of this setup for lepton flavor phenomenology, assuming that the new couplings are controlled by simple U(1) flavor models that have been proposed to accommodate large neutrino mixing angles. Remarkably, it turns out that in the context of FGM these models can pass the impressive constraints from LFV processes and leptonic electric dipole moments (EDMs) even for light superpartners, therefore offering the possibility of resolving the longstanding muon g - 2 anomaly. (orig.)

  11. LHC Signals of Pure Gravity Mediation

    Directory of Open Access Journals (Sweden)

    Feldstein Brian

    2013-05-01

    Full Text Available Evidence is mounting that natural supersymmetry at the weak scale is not realized in nature. This evidence comes from collider searches, a lack of new flavor changing neutral current effects, and now also the size of the measured Higgs mass. On the other hand, string theory suggests that supersymmetry might be present at some energy scale, and gauge coupling unification and dark matter imply that that energy scale may be relatively low. The simplest model to address all of these hints is arguably “pure gravity mediation”, in which the scalar superpartner masses are taken to be perhaps 100 TeV, with gauginos automatically acquiring loop factor suppressed masses of order TeV. The gauginos might then be the only superpartners accessible to the LHC. Unification and LSP dark matter are maintained (with a wino LSP at the cost of a 10−5 or 10−6 fine tuning. Here I will discuss the structure and LHC phenomenology of pure gravity mediation.

  12. Electron attraction mediated by Coulomb repulsion.

    Science.gov (United States)

    Hamo, A; Benyamini, A; Shapir, I; Khivrich, I; Waissman, J; Kaasbjerg, K; Oreg, Y; von Oppen, F; Ilani, S

    2016-07-21

    One of the defining properties of electrons is their mutual Coulomb repulsion. However, in solids this basic property may change; for example, in superconductors, the coupling of electrons to lattice vibrations makes the electrons attract one another, leading to the formation of bound pairs. Fifty years ago it was proposed that electrons can be made attractive even when all of the degrees of freedom in the solid are electronic, by exploiting their repulsion from other electrons. This attraction mechanism, termed 'excitonic', promised to achieve stronger and more exotic superconductivity. Yet, despite an extensive search, experimental evidence for excitonic attraction has yet to be found. Here we demonstrate this attraction by constructing, from the bottom up, the fundamental building block of the excitonic mechanism. Our experiments are based on quantum devices made from pristine carbon nanotubes, combined with cryogenic precision manipulation. Using this platform, we demonstrate that two electrons can be made to attract each other using an independent electronic system as the 'glue' that mediates attraction. Owing to its tunability, our system offers insights into the underlying physics, such as the dependence of the emergent attraction on the underlying repulsion, and the origin of the pairing energy. We also demonstrate transport signatures of excitonic pairing. This experimental demonstration of excitonic pairing paves the way for the design of exotic states of matter.

  13. [Agrobacterium-mediated transformation of Cymbidium sinensis].

    Science.gov (United States)

    Xie, Li; Wang, Fen; Zeng, Ruizhen; Guo, Herong; Zhou, Yuliang; Zhang, Zhisheng

    2015-04-01

    Genetic transformation is an effective method to improve breeding objective traits of orchids. However, there is little information about genetic transformation of Cymbidium sinensis. Rhizomes from shoot-tip culture of C. sinensis cv. 'Qijianbaimo' were used to establish a practical transformation protocol of C. sinensis. Pre-culture time, concentration and treating methods of acetosyringone, concentration of infection bacteria fluid (OD600), infection time, and co-culture time had significant effects on β-glucuronidase (GUS) transient expression rate of C. sinensis cv. 'Qijianbaimo' rhizome. The GUS transient expression rate of rhizome was the highest (11.67%) when rhizomes pre-cultured for 39 d were soaked in bacterium suspension (OD600 = 0.9) supplemented with 200 μmol/L acetosyringone for 35 min, followed by culturing on co-culture medium supplemented with 200 μmol/L acetosyringone for 7 d. Under this transformation conditions, 3 transgenic plantlets, confirmed by GUS histochemical assay and PCR, were obtained from 400 regenerated plantlets, and the genetic transformation rate was 0.75%. This proved that it was feasible to create new cultivars by the use of Agrobacterium-mediated genetic transformation in C. sinense.

  14. LHC Benchmarks from Flavored Gauge Mediation

    CERN Document Server

    Ierushalmi, N; Lee, G; Nepomnyashy, V; Shadmi, Y

    2016-01-01

    We present benchmark points for LHC searches from flavored gauge mediation models, in which messenger-matter couplings give flavor-dependent squark masses. Our examples include spectra in which a single squark - stop, scharm, or sup - is much lighter than all other colored superpartners, motivating improved quark flavor tagging at the LHC. Many examples feature flavor mixing; in particular, large stop-scharm mixing is possible. The correct Higgs mass is obtained in some examples by virtue of the large stop A-term. We also revisit the general flavor and CP structure of the models. We find that, even though the A-terms can be substantial, their contributions to EDM's are very suppressed, because of the particular dependence of the A-terms on the messenger coupling. This holds regardless of the messenger-coupling texture. More generally, the special structure of the soft terms often leads to stronger suppression of flavor- and CP-violating processes, compared to naive estimates.

  15. General Aspects of Tree Level Gauge Mediation

    CERN Document Server

    Nardecchia, Marco; Ziegler, Robert

    2009-01-01

    Tree level gauge mediation (TGM) may be considered as the simplest way to communicate supersymmetry breaking: through the tree level renormalizable exchange of heavy gauge messengers. We study its general structure, in particular the general form of tree level sfermion masses and of one loop, but enhanced, gaugino masses. This allows us to set up general guidelines for model building and to identify the hypotheses underlying the phenomenological predictions. In the context of models based on the "minimal" gauge group SO(10), we show that only two "pure" embeddings of the MSSM fields are possible using $d< 120$ representations, each of them leading to specific predictions for the ratios of family universal sfermion masses at the GUT scale, $m^2_{\\bar{5}} = 2 m^2_{10}$ or $m^2_{\\bar{5}} = (3/4) m^2_{10}$ (in SU(5) notation). These ratios are determined by group factors and are peculiar enough to make this scheme testable at the LHC. We also discuss three possible approaches to the $\\mu$-problem, one of them ...

  16. Non-universalities in pure gravity mediation

    Science.gov (United States)

    Evans, Jason L.; Ibe, Masahiro; Olive, Keith A.; Yanagida, Tsutomu T.

    2013-10-01

    The simplest model of pure gravity mediation contains only two free parameters: the gravitino mass and tan β. Scalar masses are universal at some high energy renormalization scale and gaugino masses are determined through anomalies and depend on the gravitino mass and the gauge couplings. This theory requires a relatively large gravitino mass ( m 3/2≳300 TeV) and a limited range in tan β≃1.7-2.5. Here we generalize the theory to allow for non-universality in the Higgs soft masses. This introduces zero, one or two new free parameters associated with Higgs soft masses. This generalization allows us to greatly increase the allowed range in tan β and it allows one to find viable solutions with lower m 3/2. The latter is important if we hope to find a low energy signal from gluinos. Some special cases of these non-universalities are suggestive of Higgs bosons as Nambu-Goldstone bosons or a partial no-scale structure for the Higgs doublets. Thus, we probe signatures at the weak scale and structures at the GUT and/or Planck scale.

  17. Non-Universalities in Pure Gravity Mediation

    CERN Document Server

    Evans, Jason L; Ibe, Masahiro; Yanagida, Tsutomu T

    2013-01-01

    The simplest model of pure gravity mediation contains only two free parameters: the gravitino mass and \\tan\\beta. Scalar masses are universal at some high energy renormalization scale and gaugino masses are determined through anomalies and depend on the gravitino mass and the gauge couplings. This theory requires a relatively large gravitino mass (m_{3/2}\\gtrsim 300\\,TeV) and a limited range in \\tan \\beta \\simeq 1.7--2.5. Here we generalize the theory to allow for non-universality in the Higgs soft masses. This introduces zero, one or two new free parameters associated with Higgs soft masses. This generalization allows us to greatly increase the allowed range in \\tan \\beta and it allows one to find viable solutions with lower m_{3/2}. The latter is important if we hope to find a low energy signal from gluinos. Some special cases of these non-universalities are suggestive of Higgs bosons as Nambu-Goldstone bosons or a partial no-scale structure for the Higgs doublets. Thus, we probe signatures at the weak scal...

  18. Addressing Parenting Disputes Between Estranged Parents Through Community Mediation

    Directory of Open Access Journals (Sweden)

    Madhawa Palihapitiya

    2014-07-01

    Full Text Available Mediation provides a means of resolving disputes that promises to reduce litigation while avoiding further damage to party relationships. The present study addressed the question whether parents engaged in parenting disputes generated by divorce or separation received the promised benefits of mediation when services were delivered by means of community mediation, which combined the dual features of free services and volunteer mediators. Reported outcomes such as agreements, party satisfaction, relationship changes, and court involvement, as well as the connection between these outcomes and party factors of custodial status and income level, were examined, and several encouraging trends emerged. Results indicated that an economically diverse group of disputing parents were provided access to mediation services delivered under community mediation. Moreover, the prospect of avoiding litigation motivated a majority of parents to participate in community mediation. The positive outcomes reported by most parents, irrespective of income level and custodial status, included agreement and process satisfaction rates consistent with national trends, the development of parenting plans, and for a sizable minority of parents, reduced court involvement and improved between-parent interactions. However, non-custodial parents tended to be significantly more positive about mediation’s helpfulness with parenting issues than were custodial parents.

  19. Asialoglycoprotein receptor mediated hepatocyte targeting - strategies and applications.

    Science.gov (United States)

    D'Souza, Anisha A; Devarajan, Padma V

    2015-04-10

    Hepatocyte resident afflictions continue to affect the human population unabated. The asialoglycoprotein receptor (ASGPR) is primarily expressed on hepatocytes and minimally on extra-hepatic cells. This makes it specifically attractive for receptor-mediated drug delivery with minimum concerns of toxicity. ASGPR facilitates internalization by clathrin-mediated endocytosis and exhibits high affinity for carbohydrates specifically galactose, N-acetylgalactosamine and glucose. Isomeric forms of sugar, galactose density and branching, spatial geometry and galactose linkages are key factors influencing ligand-receptor binding. Popular ligands for ASGPR mediated targeting are carbohydrate polymers, arabinogalactan and pullulan. Other ligands include galactose-bearing glycoproteins, glycopeptides and galactose modified polymers and lipids. Drug-ligand conjugates provide a viable strategy; nevertheless ligand-anchored nanocarriers provide an attractive option for ASGPR targeted delivery and are widely explored. The present review details various ligands and nanocarriers exploited for ASGPR mediated delivery of drugs to hepatocytes. Nanocarrier properties affecting ASGPR mediated uptake are discussed at length. The review also highlights the clinical relevance of ASGPR mediated targeting and applications in diagnostics. ASGPR mediated hepatocyte targeting provides great promise for improved therapy of hepatic afflictions.

  20. Fungal mediator tail subunits contain classical transcriptional activation domains.

    Science.gov (United States)

    Liu, Zhongle; Myers, Lawrence C

    2015-04-01

    Classical activation domains within DNA-bound eukaryotic transcription factors make weak interactions with coactivator complexes, such as Mediator, to stimulate transcription. How these interactions stimulate transcription, however, is unknown. The activation of reporter genes by artificial fusion of Mediator subunits to DNA binding domains that bind to their promoters has been cited as evidence that the primary role of activators is simply to recruit Mediator. We have identified potent classical transcriptional activation domains in the C termini of several tail module subunits of Saccharomyces cerevisiae, Candida albicans, and Candida dubliniensis Mediator, while their N-terminal domains are necessary and sufficient for their incorporation into Mediator but do not possess the ability to activate transcription when fused to a DNA binding domain. This suggests that Mediator fusion proteins actually are functioning in a manner similar to that of a classical DNA-bound activator rather than just recruiting Mediator. Our finding that deletion of the activation domains of S. cerevisiae Med2 and Med3, as well as C. dubliniensis Tlo1 (a Med2 ortholog), impairs the induction of certain genes shows these domains function at native promoters. Activation domains within coactivators are likely an important feature of these complexes and one that may have been uniquely leveraged by a common fungal pathogen.

  1. The Mediatization of Politics. From the National to the Transnational

    Directory of Open Access Journals (Sweden)

    Hans-Jörg Trenz

    2014-11-01

    Full Text Available The mediatization of politics is generally explained in relation to the legitimacy requirements of the modern state and as such, it is typically confined to the national media sphere. Can we speak in any meaningful way of mediatization beyond the national? The European Union (EU, which operates under increasing legitimacy constraints and is exposed to the salience of media debates that contest its public legitimacy, is a case in point. Is the EU becoming mediatized? And what are the effects of EU mediatization? Under what conditions can the mass media become a facilitator of European integration? The issue at stake is whether the media (new and old can have an integration function beyond the national and facilitate the building of democratic legitimacy of the European Union. We propose that the concept of mediatization offers the theoretical and analytical tools necessary to understand precisely how the interaction between the EU polity and the media unfolds and how it impacts on the process of the EU’s public legitimation. First we deliver a general account of mediatization, highlighting its core definers and main points of critique that the concept has attracted. We then show how mediatization is relevant to the EU pol­ity and propose an analytical model that can capture this process empirically

  2. Inlfammatory mediators and microcirculatory disturbance in acute pancreatitis

    Institute of Scientific and Technical Information of China (English)

    Xi-Ping Zhang; Zhi-Jun Li; Jie Zhang

    2009-01-01

    BACKGROUND: Inlfammatory mediators are not only initiation factors of acute pancreatitis (AP) but also key factors causing pancreatic hemorrhage and necrosis, which damage important organs such as the heart, brain, liver, kidney and lung. Microcirculatory disturbance in AP has attracted widespread attention. In order to provide a theoretical basis for clinical therapy of AP, it is very important to explore the effect of inlfammatory mediators on microcirculatory disturbance in this disease. DATA SOURCES: In this review, the impact of inlfammatory mediators on microcirculatory disturbance in AP was reviewed according to the literature, especially the articles indexed in PubMed and books published in China and reports from websites. RESULTS: At present, inlfammatory mediation and micro-circulatory disturbance are the two major hypotheses to explain the development of AP. Although experimental studies have shown that inlfammatory mediators induce or aggravate microcirculatory disturbance, the clinical application of these ifndings is still dififcult because the inlfammatory mediators are diverse and their research is not comprehensive and thorough. CONCLUSION: It is very important to explore the inlfuence of inlfammatory mediators on microcirculatory disturbance in AP.

  3. Mammalian mediator 19 mediates H1299 lung adenocarcinoma cell clone conformation, growth, and metastasis.

    Science.gov (United States)

    Xu, Lu-Lu; Guo, Shu-Liang; Ma, Su-Ren; Luo, Yong-Ai

    2012-01-01

    Mammalian mediator (MED) is a multi-protein coactivator that has been identified by several research groups. The involvement of the MED complex subunit 19 (MED 19) in the metastasis of lung adenocarcinoma cell line (H1299), which expresses the MED 19 subunit, was here investigated. When MED 19 expression was decreased by RNA interference H1299 cells demonstrated reduced clone formation, arrest in the S phase of the cell cycle, and lowered metastatic capacity. Thus, MED 19 appears to play important roles in the biological behavior of non-small cell lung carcinoma cells. These findings may be important for the development of novel lung carcinoma treatments.

  4. Gamma-ray signal from Dark Matter Annihilation mediated by mixing scalar mediators

    CERN Document Server

    Teng, Fei

    2016-01-01

    We present here a study of the direct and indirect detection prospects of a generic dark matter simplified model, in which the Majorana dark matter interacts only with a Standard Model lepton and a pair of uncolored mixing scalar mediators. We first show that the mixing angle significantly changes the feature of internal bremsstrahlung, as well as the flux ratio of $\\gamma\\gamma$ and $\\gamma Z$ line signals. The $CP$-violation phase will introduce an polarization asymmetry in the $\\gamma\\gamma$ final state. Then we study the direct detection prospect and discuss the complimentarity of these two search strategies.

  5. Metastable Supersymmetry Breaking and Minimal Gauge Mediation on Branes

    CERN Document Server

    Halyo, Edi

    2010-01-01

    We construct a model with D5 branes wrapped on a deformed and resolved $A_6$ singularity which realizes metastable supersymmetry breaking and minimal gauge mediation. Supersymmetry is broken at tree level by the F--term of singlet which also obtains a VEV as required in gauge mediation. Three nodes of the singularity are used to break supersymmetry whereas the other three realize gauge mediation. The supersymmetry breaking scale is suppressed due to brane instanton effects which are computed using a geometric transition.

  6. CRYPTOCHROME mediates behavioral executive choice in response to UV light

    Science.gov (United States)

    Baik, Lisa S.; Fogle, Keri J.; Roberts, Logan; Galschiodt, Alexis M.; Chevez, Joshua A.; Recinos, Yocelyn; Nguy, Vinh; Holmes, Todd C.

    2017-01-01

    Drosophila melanogaster CRYPTOCHROME (CRY) mediates behavioral and electrophysiological responses to blue light coded by circadian and arousal neurons. However, spectroscopic and biochemical assays of heterologously expressed CRY suggest that CRY may mediate functional responses to UV-A (ultraviolet A) light as well. To determine the relative contributions of distinct phototransduction systems, we tested mutants lacking CRY and mutants with disrupted opsin-based phototransduction for behavioral and electrophysiological responses to UV light. CRY and opsin-based external photoreceptor systems cooperate for UV light-evoked acute responses. CRY mediates behavioral avoidance responses related to executive choice, consistent with its expression in central brain neurons. PMID:28062690

  7. How a single photon can mediate entanglement between two others

    Science.gov (United States)

    de Lima Bernardo, Bertúlio

    2016-10-01

    We describe a novel quantum information protocol, which probabilistically entangles two distant photons that have never interacted. Different from the entanglement swapping protocol, which requires two pairs of maximally entangled photons as the input states, as well as a Bell-state measurement (BSM), the present scheme only requires three photons: two to be entangled and another to mediate the correlation, and no BSM, in a process that we call "entanglement mediation". Furthermore, in analyzing the paths of the photons in our arrangement, we conclude that one of them, the mediator, exchanges information with the two others simultaneously, which seems to be a new quantum-mechanical feature.

  8. Intelligent query processing for semantic mediation of information systems

    Directory of Open Access Journals (Sweden)

    Saber Benharzallah

    2011-11-01

    Full Text Available We propose an intelligent and an efficient query processing approach for semantic mediation of information systems. We propose also a generic multi agent architecture that supports our approach. Our approach focuses on the exploitation of intelligent agents for query reformulation and the use of a new technology for the semantic representation. The algorithm is self-adapted to the changes of the environment, offers a wide aptitude and solves the various data conflicts in a dynamic way; it also reformulates the query using the schema mediation method for the discovered systems and the context mediation for the other systems.

  9. Contentious Conversations: Using Mediation Techniques in Difficult Clinical Ethics Consultations.

    Science.gov (United States)

    Fiester, Autumn

    2015-01-01

    Mediators utilize a wide range of skills in the process of facilitating dialogue and resolving conflicts. Among the most useful techniques for clinical ethics consultants (CECs)-and surely the least discussed-are those employed in acrimonious, hostile conversations between stakeholders. In the context of clinical ethics disputes or other bedside conflicts, good mediation skills can reverse the negative interactions that have prevented the creation of workable treatment plans or ethical consensus. This essay lays out the central framework mediators use in distinguishing positions from interests and describes a set of strategies for managing contentious ethics consultations or working with "difficult" patients, families, or patient-careprovider interactions.

  10. Non-minimal gauge mediation and moduli stabilization

    Energy Technology Data Exchange (ETDEWEB)

    Jelinski, T.; Lalak, Z. [Institute of Theoretical Physics, University of Warsaw, ul. Hoza 69, 00-681 Warsaw (Poland); Pawelczyk, J., E-mail: jacek.pawelczyk@fuw.edu.p [Institute of Theoretical Physics, University of Warsaw, ul. Hoza 69, 00-681 Warsaw (Poland)

    2010-05-31

    In this Letter we consider U(1){sub A}-gauged Polonyi model with two spurions coupled to a twisted closed string modulus. This offers a consistent setup for metastable SUSY breakdown which allows for moduli stabilization and naturally leads to gauge or hybrid gauge/gravitational mediation mechanism. Due to the presence of the second spurion one can arrange for a solution of the {mu} and B{sub {mu}}problems in a version of modified Giudice-Masiero mechanism, which works both in the limit of pure gauge mediation and in the mixed regime of hybrid mediation.

  11. Neuroprotection from complement-mediated inflammatory damage.

    Science.gov (United States)

    Kulkarni, Amod P; Kellaway, Laurie A; Lahiri, Debomoy K; Kotwal, Girish J

    2004-12-01

    Several neurodegenerative disorders, such as multiple sclerosis, Alzheimer's disease, and Parkinson's disease, are associated with inflammatory damage. The complex process of neuroinflammation involves various components of the immune system and the central nervous system. Particularly, brain astrocytes and microglial cells generate several inflammatory mediators like cytokines, leukotrienes, superoxide radicals, eicasonoids, and the components of the complement cascade. Complement plays an important role in the etiology of most of the neuroinflammatory disorders. To prevent long-term dysfunction inflammation in the central nervous system must be modulated with neuroprotective agents such as nonsteroidal anti-inflammatory drugs, steroids, phenolic thiazoles, nitrones, catechins, nitric oxide synthetase inhibitors, flavonoids, and phosphodiesterase inhibitors. Few drugs are found to be effective and their therapeutic benefit is hampered by side effects. Most of the neuroprotective agents are free radical scavengers and many inhibit only one or two aspects of inflammation. The complement inhibitory activity of most of these agents is either unknown or not established. Thus, there is doubt regarding their therapeutic value in most of the inflammatory disorders in which complement plays a major role. In this context the role of a multifunctional protein, vaccinia virus complement control protein (VCP), is quite significant as it may play a pivotal role in the treatment of several neuroinflammatory disorders. VCP is known to inhibit both complement pathways involved in inflammation. It is also known to inhibit cytokines and chemokines in inflammation. Our recent studies on rats demonstrate that VCP administration inhibits macrophage infiltration, reduces spinal cord destruction, and improves motor skills associated with spinal cord injury, establishing VCP as a strong candidate for neuroprotection. Thus, complement inhibitors such as VCP can serve as neuroprotective

  12. Efflux pump-mediated resistance in chemotherapy.

    Science.gov (United States)

    Ughachukwu, Po; Unekwe, Pc

    2012-07-01

    Efflux pump mechanisms perform important physiological functions such as prevention of toxin absorption from the gastrointestinal tract, elimination of bile from the hepatocytes, effective functioning of the blood-brain barrier and placental barrier, and renal excretion of drugs. They exist in all living cells, but those in the bacterial and mammalian cells are more important to the clinician and pharmacologist, as they constitute an important cause of antimicrobial drug resistance, which contributes to treatment failure, high medical bills, and increased mortality / morbidity. This review was aimed at highlighting the role of efflux pump mechanisms in microbial resistance to chemotherapeutic agents. It was also aimed to elucidate their structure and mechanisms of action so as to integrate the efflux pump mechanisms in the design and development of novel antimicrobial agents. Findings from previous studies and research on this subject assessed through Google search, Pubmed, Hinari websites, as well as standard textbooks on chemotherapy, provided the needed information in the process of this review. Efflux pump inhibitors are promising strategies for preventing and reverting efflux-mediated resistance to chemotherapeutic agents. They are usually employed as adjuncts in antimicrobial and cancer chemotherapy. Toxicity, more common with the older-generation inhibitors such as verapamil and reserpine, constitutes the greatest impediment to their clinical applications. No efflux pump inhibitor has been approved for routine clinical use, as a result of doubtful clinical efficacy and unacceptably high incidence of adverse effects, particularly inhibition of the P-450 drug metabolizing enzyme. At present, their applications are mainly restricted to epidemiological studies. Nonetheless, the search for efficacious and tolerable efflux pump inhibitors continues because of the potential benefits. There is a need to consider efflux pump substrate selectivity in the design and

  13. Proinflammatory mediators stimulate neutrophil-directed angiogenesis.

    LENUS (Irish Health Repository)

    McCourt, M

    2012-02-03

    BACKGROUND: Vascular endothelial growth factor (VEGF; vascular permeability factor) is one of the most potent proangiogenic cytokines, and it plays a central role in mediating the process of angiogenesis or new blood vessel formation. Neutrophils (PMNs) recently have been shown to produce VEGF. HYPOTHESIS: The acute inflammatory response is a potent stimulus for PMN-directed angiogenesis. METHODS: Neutrophils were isolated from healthy volunteers and stimulated with lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6), and anti-human Fas monoclonal antibody. Culture supernatants were assayed for VEGF using enzyme-linked immunosorbent assays. Culture supernatants from LPS- and TNF-alpha-stimulated PMNs were then added to human umbilical vein endothelial cells and human microvessel endothelial cells and assessed for endothelial cell proliferation using 5-bromodeoxyuridine labeling. Tubule formation was also assessed on MATRIGEL basement membrane matrix. Neutrophils were lysed to measure total VEGF release, and VEGF expression was detected using Western blot analysis. RESULTS: Lipopolysaccharide and TNF-alpha stimulation resulted in significantly increased release of PMN VEGF (532+\\/-49 and 484+\\/-80 pg\\/mL, respectively; for all, presented as mean +\\/- SEM) compared with control experiments (32+\\/-4 pg\\/mL). Interleukin 6 and Fas had no effect. Culture supernatants from LPS- and TNF-alpha-stimulated PMNs also resulted in significant increases (P<.005) in macrovascular and microvascular endothelial cell proliferation and tubule formation. Adding anti-human VEGF-neutralizing polyclonal antibody to stimulated PMN supernatant inhibited these effects. Total VEGF release following cell lysis and Western blot analysis suggests that the VEGF is released from an intracellular store. CONCLUSION: Activated human PMNs are directly angiogenic by releasing VEGF, and this has important implications for inflammation, capillary leak syndrome

  14. Nanomedicine-mediated cancer stem cell therapy.

    Science.gov (United States)

    Shen, Song; Xia, Jin-Xing; Wang, Jun

    2016-01-01

    Circumstantial evidence suggests that most tumours are heterogeneous and contain a small population of cancer stem cells (CSCs) that exhibit distinctive self-renewal, proliferation and differentiation capabilities, which are believed to play a crucial role in tumour progression, drug resistance, recurrence and metastasis in multiple malignancies. Given that the existence of CSCs is a primary obstacle to cancer therapy, a tremendous amount of effort has been put into the development of anti-CSC strategies, and several potential approaches to kill therapeutically-resistant CSCs have been explored, including inhibiting ATP-binding cassette transporters, blocking essential signalling pathways involved in self-renewal and survival of CSCs, targeting CSCs surface markers and destroying the tumour microenvironment. Meanwhile, an increasing number of therapeutic agents (e.g. small molecule drugs, nucleic acids and antibodies) to selectively target CSCs have been screened or proposed in recent years. Drug delivery technology-based approaches hold great potential for tackling the limitations impeding clinical applications of CSC-specific agents, such as poor water solubility, short circulation time and inconsistent stability. Properly designed nanocarrier-based therapeutic agents (or nanomedicines) offer new possibilities of penetrating CSC niches and significantly increasing therapeutic drug accumulation in CSCs, which are difficult for free drug counterparts. In addition, intelligent nanomedicine holds great promise to overcome pump-mediated multidrug resistance which is driven by ATP and to decrease detrimental effects on normal somatic stem cells. In this review, we summarise the distinctive biological processes related to CSCs to highlight strategies against inherently drug-resistant CSCs. We then focus on some representative examples that give a glimpse into state-of-the-art nanomedicine approaches developed for CSCs elimination. A perspective on innovative therapeutic

  15. KSHV-Mediated Angiogenesis in Tumor Progression

    Directory of Open Access Journals (Sweden)

    Pravinkumar Purushothaman

    2016-07-01

    Full Text Available Human herpesvirus 8 (HHV-8, also known as Kaposi’s sarcoma-associated herpesvirus (KSHV, is a malignant human oncovirus belonging to the gamma herpesvirus family. HHV-8 is closely linked to the pathogenesis of Kaposi’s sarcoma (KS and two other B-cell lymphoproliferative diseases: primary effusion lymphoma (PEL and a plasmablastic variant of multicentric Castleman’s disease (MCD. KS is an invasive tumor of endothelial cells most commonly found in untreated HIV-AIDS or immuno-compromised individuals. KS tumors are highly vascularized and have abnormal, excessive neo-angiogenesis, inflammation, and proliferation of infected endothelial cells. KSHV directly induces angiogenesis in an autocrine and paracrine fashion through a complex interplay of various viral and cellular pro-angiogenic and inflammatory factors. KS is believed to originate due to a combination of KSHV’s efficient strategies for evading host immune systems and several pro-angiogenic and pro-inflammatory stimuli. In addition, KSHV infection of endothelial cells produces a wide array of viral oncoproteins with transforming capabilities that regulate multiple host-signaling pathways involved in the activation of angiogenesis. It is likely that the cellular-signaling pathways of angiogenesis and lymph-angiogenesis modulate the rate of tumorigenesis induction by KSHV. This review summarizes the current knowledge on regulating KSHV-mediated angiogenesis by integrating the findings reported thus far on the roles of host and viral genes in oncogenesis, recent developments in cell-culture/animal-model systems, and various anti-angiogenic therapies for treating KSHV-related lymphoproliferative disorders.

  16. KSHV-Mediated Angiogenesis in Tumor Progression

    Science.gov (United States)

    Purushothaman, Pravinkumar; Uppal, Timsy; Sarkar, Roni; Verma, Subhash C.

    2016-01-01

    Human herpesvirus 8 (HHV-8), also known as Kaposi’s sarcoma-associated herpesvirus (KSHV), is a malignant human oncovirus belonging to the gamma herpesvirus family. HHV-8 is closely linked to the pathogenesis of Kaposi’s sarcoma (KS) and two other B-cell lymphoproliferative diseases: primary effusion lymphoma (PEL) and a plasmablastic variant of multicentric Castleman’s disease (MCD). KS is an invasive tumor of endothelial cells most commonly found in untreated HIV-AIDS or immuno-compromised individuals. KS tumors are highly vascularized and have abnormal, excessive neo-angiogenesis, inflammation, and proliferation of infected endothelial cells. KSHV directly induces angiogenesis in an autocrine and paracrine fashion through a complex interplay of various viral and cellular pro-angiogenic and inflammatory factors. KS is believed to originate due to a combination of KSHV’s efficient strategies for evading host immune systems and several pro-angiogenic and pro-inflammatory stimuli. In addition, KSHV infection of endothelial cells produces a wide array of viral oncoproteins with transforming capabilities that regulate multiple host-signaling pathways involved in the activation of angiogenesis. It is likely that the cellular-signaling pathways of angiogenesis and lymph-angiogenesis modulate the rate of tumorigenesis induction by KSHV. This review summarizes the current knowledge on regulating KSHV-mediated angiogenesis by integrating the findings reported thus far on the roles of host and viral genes in oncogenesis, recent developments in cell-culture/animal-model systems, and various anti-angiogenic therapies for treating KSHV-related lymphoproliferative disorders. PMID:27447661

  17. Peccei-Quinn symmetric pure gravity mediation

    Energy Technology Data Exchange (ETDEWEB)

    Evans, Jason L.; Olive, Keith A. [University of Minnesota, William I. Fine Theoretical Physics Institute, School of Physics and Astronomy, Minneapolis, MN (United States); Ibe, Masahiro [University of Tokyo, ICRR, Kashiwa (Japan); University of Tokyo, Kavli IPMU (WPI), TODIAS, Kashiwa (Japan); Yanagida, Tsutomu T. [University of Tokyo, Kavli IPMU (WPI), TODIAS, Kashiwa (Japan)

    2014-07-15

    Successful models of pure gravity mediation (PGM) with radiative electroweak symmetry breaking can be expressed with as few as two free parameters, which can be taken as the gravitinomass and tan β. These models easily support a 125-126 GeV Higgs mass at the expense of a scalar spectrum in the multi-TeV range and a much lighter wino as the lightest supersymmetric particle. In these models, it is also quite generic that the Higgs mixing mass parameter, μ, which is determined by the minimization of the Higgs potential is also in the multi-TeV range. For μ > 0, the thermal relic density of winos is too small to account for the darkmatter. The same is true for μ < 0 unless the gravitinomass is of order 500 TeV. Here, we consider the origin of a multi-TeV μ parameter arising from the breakdown of a Peccei-Quinn (PQ) symmetry. A coupling of the PQ-symmetry breaking field, P, to the MSSM Higgs doublets, naturally leads to a value of μ ∝ left angle P right angle {sup 2}/M{sub P} ∝ O(100) TeV and of the order that is required in PGM models. In this case, axions make up the dark matter or some fraction of the dark matter with the remainder made up from thermal or non-thermal winos. We also provide solutions to the problem of isocurvature fluctuations with axion dark matter in this context. (orig.)

  18. Treatment of immune-mediated, dysimmune neuropathies.

    Science.gov (United States)

    Finsterer, J

    2005-08-01

    This review focuses on the actual status and recent advances in the treatment of immune-mediated neuropathies, including: Guillain-Barre syndrome (GBS) with its subtypes acute inflammatory demyelinating polyradiculoneuropathy, acute motor axonal neuropathy, acute motor and sensory axonal neuropathy, Miller Fisher syndrome, and acute pandysautonomia; chronic inflammatory demyelinating polyneuropathy (CIDP) with its subtypes classical CIDP, CIDP with diabetes, CIDP/monoclonal gammopathy of undetermined significance (MGUS), sensory CIDP, multifocal motor neuropathy (MMN), multifocal acquired demyelinating sensory and motor neuropathy or Lewis-Sumner syndrome, multifocal acquired sensory and motor neuropathy, and distal acquired demyelinating sensory neuropathy; IgM monoclonal gammopathies with its subtypes Waldenstrom's macroglobulinemia, myelin-associated glycoprotein-associated gammopathy, polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes syndrome, mixed cryoglobulinemia, gait ataxia, late-onset polyneuropathy syndrome, and MGUS. Concerning the treatment of GBS, there is no significant difference between intravenous immunoglobulins (IVIG), plasma exchange or plasma exchange followed by IVIG. Because of convenience and absent invasiveness, IVIG are usually preferred. In treating CIDP corticosteroids, IVIG, or plasma exchange are equally effective. Despite the high costs and relative lack of availability, IVIG are preferentially used. For the one-third of patients, who does not respond, other immunosuppressive options are available. In MMN IVIG are the treatment of choice. Inadequate response in 20% of the patients requires adjunctive immunosuppressive therapies. Neuropathies with IgM monoclonal gammopathy may respond to various chemotherapeutic agents, although the long-term effects are unknown. In addition, such treatment may be associated with serious side effects. Recent data support the use of rituximab, a monoclonal antibody against the B

  19. Moral mediation in interpreted health care consultations.

    Science.gov (United States)

    Seale, Clive; Rivas, Carol; Al-Sarraj, Hela; Webb, Sarah; Kelly, Moira

    2013-12-01

    This paper reports on the moral work done in routine diabetes review consultations in primary care with nurses. Consultations with fluent English speakers are compared with consultations where an interpreter was present, largely involving patients of Bangladeshi origin. The study setting was Tower Hamlets in London, where type 2 diabetes is particularly common. Existing research has shown some dissatisfaction with diabetes care amongst Bangladeshi patients, and studies of care providers in other locations suggest that they at times experience the care of this group as particularly challenging. Through analysis of video-recorded consultations recorded in 2010-2011 we shed light on possible reasons for these difficulties. The 12 non-English speakers often experienced difficulties in raising issues that concerned them, particularly if their interpreter did not translate their utterance because it was deemed to be unrelated to diabetes. These difficulties were not shared by the 24 fluent English speakers, who also found it easier to convey a positive moral reputation and to excuse behaviour that deviated from recommended self-management practices. Interpreters at times also acted as moral mediators. For example, where a participant in the consultation made statements that appeared to convey a negative moral judgement of an other participant, these would often go untranslated. Probably, neither health care providers nor patients are fully aware of the nature of their communication difficulties. Given this, interpreters possess considerable power to influence matters. Understanding the moral work of consultations is important in explaining the findings of other studies showing difficulties in the provision of diabetes care to people with limited English language skills.

  20. Mediation analysis when a continuous mediator is measured with error and the outcome follows a generalized linear model.

    Science.gov (United States)

    Valeri, Linda; Lin, Xihong; VanderWeele, Tyler J

    2014-12-10

    Mediation analysis is a popular approach to examine the extent to which the effect of an exposure on an outcome is through an intermediate variable (mediator) and the extent to which the effect is direct. When the mediator is mis-measured, the validity of mediation analysis can be severely undermined. In this paper, we first study the bias of classical, non-differential measurement error on a continuous mediator in the estimation of direct and indirect causal effects in generalized linear models when the outcome is either continuous or discrete and exposure-mediator interaction may be present. Our theoretical results as well as a numerical study demonstrate that in the presence of non-linearities, the bias of naive estimators for direct and indirect effects that ignore measurement error can take unintuitive directions. We then develop methods to correct for measurement error. Three correction approaches using method of moments, regression calibration, and SIMEX are compared. We apply the proposed method to the Massachusetts General Hospital lung cancer study to evaluate the effect of genetic variants mediated through smoking on lung cancer risk.

  1. Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration

    Science.gov (United States)

    Zhang, Jian-Nan; Michel, Uwe; Lenz, Christof; Friedel, Caroline C.; Köster, Sarah; d’Hedouville, Zara; Tönges, Lars; Urlaub, Henning; Bähr, Mathias; Lingor, Paul; Koch, Jan C.

    2016-01-01

    Axonal degeneration is a key initiating event in many neurological diseases. Focal lesions to axons result in a rapid disintegration of the perilesional axon by acute axonal degeneration (AAD) within several hours. However, the underlying molecular mechanisms of AAD are only incompletely understood. Here, we studied AAD in vivo through live-imaging of the rat optic nerve and in vitro in primary rat cortical neurons in microfluidic chambers. We found that calpain is activated early during AAD of the optic nerve and that calpain inhibition completely inhibits axonal fragmentation on the proximal side of the crush while it attenuates AAD on the distal side. A screening of calpain targets revealed that collapsin response mediator protein-2 (CRMP2) is a main downstream target of calpain activation in AAD. CRMP2-overexpression delayed bulb formation and rescued impairment of axonal mitochondrial transport after axotomy in vitro. In vivo, CRMP2-overexpression effectively protected the proximal axon from fragmentation within 6 hours after crush. Finally, a proteomic analysis of the optic nerve was performed at 6 hours after crush, which identified further proteins regulated during AAD, including several interactors of CRMP2. These findings reveal CRMP2 as an important mediator of AAD and define it as a putative therapeutic target. PMID:27845394

  2. A new scalar mediated WIMPs with pairs of on-shell mediators in annihilations

    CERN Document Server

    Jia, Lian-Bao

    2016-01-01

    In this article, we focus on a new scalar $\\phi$ mediated scalar/vectoral WIMPs (weakly interacting massive particles). To explain the Galactic center 1 - 3 GeV gamma-ray excess, here we consider the case that a WIMP pair predominantly annihilates into an on-shell $\\phi \\phi$ pair which mainly decays to $\\tau \\bar{\\tau}$, with masses of WIMPs in a range about 14 - 22 GeV. For the mass of $\\phi$ slightly below the WIMP mass, the annihilations of WIMPs are phase space suppressed today, and the required thermally averaged annihilation cross section of WIMPs can be derived to meet the GeV gamma-ray excess. A small scalar mediator-Higgs field mixing is introduced, which is available in interpreting the GeV gamma-ray excess. With the constraints of the dark matter relic density, the indirect detection result, the collider experiment, the thermal equilibrium of the early universe and the dark matter direct detection experiment are considered, we find there are parameter spaces left. The WIMPs may be detectable at th...

  3. Intergroup differentiation in computer-mediated communication : Effects of depersonalization

    NARCIS (Netherlands)

    Postmes, T; Spears, R; Lea, M

    2002-01-01

    Two studies examined intergroup discussions via computer-mediated communication systems. It was hypothesized that depersonalization, in comparison with individuated interaction, would increase the tendency for intergroup differentiation in attitudes and stereotypes, In Study 1, 24 groups communicate

  4. STAT6: its role in interleukin 4-mediated biological functions.

    Science.gov (United States)

    Takeda, K; Kishimoto, T; Akira, S

    1997-05-01

    Interleukin (IL) 4 is known to be a cytokine which plays a central role in the regulation of immune response. Studies on cytokine signal transduction have clarified the mechanism by which IL4 exerts its functions. Two cytoplasmic proteins, signal transducer and activator of transcription (STAT) 6 and IL4-induced phosphotyrosine substrate/insulin receptor substrate 2 (4PS/IRS2), are activated in IL4 signal transduction. Recent studies from STAT6-deficient mice have revealed the essential role of STAT6 in IL4-mediated biological actions. In addition, STAT6 has also been demonstrated to be important for the functions mediated by IL13, which is related to IL4. IL4 and IL13 have been shown to induce the production of IgE, which is a major mediator in an allergic response. These findings indicate that STAT6 activation is involved in IL4- and IL13-mediated disorders such as allergy.

  5. Neuroprotective Activity of (--Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity

    Directory of Open Access Journals (Sweden)

    Jin-Biao Liu

    2016-01-01

    Full Text Available Lipopolysaccharide- (LPS- mediated systemic inflammation plays a critical role in neurodegenerative diseases. The present study was conducted to evaluate the protective effects of epigallocatechin gallate (EGCG, the major component in green tea, on LPS-mediated inflammation and neurotoxicity. LPS treatment of macrophages induced expression of proinflammatory cytokines (TNF-α, IL-1β, and IL-6. However, EGCG pretreatment of macrophages significantly inhibited LPS-mediated induction of these cytokines. In addition, EGCG significantly diminished LPS-induced inflammatory cytokines in the peripheral mononuclear blood cells (PBMCs. Supernatant from EGCG-pretreated and LPS-activated macrophage cultures was found to be less cytotoxic to neurons than that from non-EGCG-pretreated and LPS-activated macrophage cultures. Furthermore, EGCG treatment of neurons could inhibit LPS-induced production of reactive oxygen species (ROS. Thus EGCG represents a potent and useful neuroprotective agent for inflammation-mediated neurological disorders.

  6. Inducible nitric oxide synthase mediates bone loss in ovariectomized mice.

    NARCIS (Netherlands)

    Cuzzocrea, S.; Mazzon, E.; Dugo, L.; Genovese, T.; Paola, R. Di; Ruggeri, Z.; Vegeto, E.; Caputi, A.P.; Loo, F.A.J. van de; Puzzolo, D.; Maggi, A.

    2003-01-01

    Several clinical studies have shown that bone loss may be attributed to osteoclast recruitment induced by mediators of inflammation. In different experimental paradigms we have recently demonstrated that estrogen exhibits antiinflammatory activity by preventing the induction of inducible nitric oxid

  7. Host plant quality mediates competition between arbuscular mycorrhizal fungi

    NARCIS (Netherlands)

    Knegt, B.; Jansa, J.; Franken, O.; Engelmoer, D.J.P.; Werner, G.D.A.; Bücking, H.; Kiers, E.T.

    2016-01-01

    Arbuscular mycorrhizal fungi exchange soil nutrients for carbon from plant hosts. Empirical works suggests that hosts may selectively provide resources to different fungal species, ultimately affecting fungal competition. However, fungal competition may also be mediated by colonization strategies of

  8. Host plant quality mediates competition between arbuscular mycorrhizal fungi

    NARCIS (Netherlands)

    B. Knegt; J. Jansa; O. Franken; D.J.P. Engelmoer; G.D.A. Werner; H. Bücking; E.T. Kiers

    2014-01-01

    Arbuscular mycorrhizal fungi exchange soil nutrients for carbon from plant hosts. Empirical works suggests that hosts may selectively provide resources to different fungal species, ultimately affecting fungal competition. However, fungal competition may also be mediated by colonization strategies of

  9. Lay Theories Regarding Computer-Mediated Communication in Remote Collaboration

    Directory of Open Access Journals (Sweden)

    Karl Parke

    2017-03-01

    Full Text Available Computer-mediated communication and remote collaboration has become an unexceptional norm as an educational modality for distance and open education, therefore the need to research and analyze students' online learning experience is necessary. This paper seeks to examine the assumptions and expectations held by students in regard to computer-mediated communication and how their lay theories developed and changed within the context of their practical experiences in conducting a remote collaborative project, through computer-mediated communication. We conducted a qualitative content analysis of students' final reports from an inter-institutional online course on computer-mediated communication and remote collaboration. The results show that students’ assumptions were altered and indicate the strong benefits of teaching how to collaborate remotely, especially if a blended approach of theory and practical application are combined.

  10. Wide-Area Computer-Mediated Communication in Business Writing.

    Science.gov (United States)

    Howard, Tharon

    1992-01-01

    Discusses the differences between computer-mediated communication (CMC) and print or oral communication. Describes advantages and problems of using electronic mail in a business context. Recommends research and teaching of CMC in the business writing classroom. (MM)

  11. Non-IgE mediated mast cell activation.

    Science.gov (United States)

    Yu, Yingxin; Blokhuis, Bart R; Garssen, Johan; Redegeld, Frank A

    2016-05-05

    Mast cells are crucial effector cells in allergic reactions, where IgE is the best known mechanism to trigger their degranulation and release of a vast array of allergic mediators. However, IgE is not the only component to stimulate these cells to degranulate, while mast cell activation can also result in differential release of mediators. There is a plethora of stimuli, such as IgG, complement components, TLR ligands, neuropeptides, cytokines, chemokines and other inflammatory products, that can directly trigger mast cell degranulation, cause selective release of mediators, and stimulate proliferation, differentiation and/or migration. Moreover, some of these stimuli have a synergic effect on the IgE-mediated mast cell activation. Because of the ability to respond to a large repertoire of stimuli, mast cells may act as a versatile cell in various physiological and pathological conditions. In this review, we discuss current knowledge on non-IgE stimuli for (human) mast cells.

  12. Parents’ mediation of formal thinking: A try of operationalization

    Directory of Open Access Journals (Sweden)

    Stepanović Ivana

    2006-01-01

    Full Text Available One possible way of examining parents’ mediation of formal thinking has been presented. The specific goal was to construct a scale which represents specific means of parents’ mediation that appear in their everyday communication with children. Piaget’s theory i.e. main characteristics of formal operations and concepts from Vygotsky’s paradigm, especially mediation means concept, served as theoretical background for scale constructing. The similarities between final stages of cognitive development within these two approaches have been analyzed. The research was conducted in which Crombach’s alpha was examined as an indicator of scale quality as well as correlation between children’s answers on the scale and their achievement on formal operations test. The most relevant aspects of parents’ mediation were extracted and analyzed.

  13. Computer-Mediated Materials for Chinese Character Learning.

    Science.gov (United States)

    Hsu, Hui-Mei; Gao, Liwei

    2002-01-01

    Reviews four sets of computer-mediated materials for Chinese character learning. These include the following: Write Chinese, Chinese Characters Primer, Animated Chinese Characters, and USC Chinese Character Page. (Author/VWL)

  14. Real-time Control Mediation in Agile Distributed Software Development

    DEFF Research Database (Denmark)

    Persson, John Stouby; Aaen, Ivan; Mathiassen, Lars

    2008-01-01

    Agile distributed environments pose particular challenges related to control of quality and collaboration in software development. Moreover, while face-to-face interaction is fundamental in agile development, distributed environments must rely extensively on mediated interactions. On this backdro...

  15. Theories of Learning and Computer-Mediated Instructional Technologies.

    Science.gov (United States)

    Hung, David

    2001-01-01

    Describes four major models of learning: behaviorism, cognitivism, constructivism, and social constructivism. Discusses situated cognition; differences between learning theories and instructional approaches; and how computer-mediated technologies can be integrated with learning theories. (LRW)

  16. Antibody-Mediated Immunity against Tuberculosis: Implications for Vaccine Development

    OpenAIRE

    Achkar, Jacqueline M; Casadevall, Arturo

    2013-01-01

    There is an urgent need for new and better vaccines against tuberculosis (TB). Current vaccine design strategies are generally focused on the enhancement of cell-mediated immunity. Antibody-based approaches are not being considered, mostly due to the paradigm that humoral immunity plays little role in the protection against intracellular pathogens. Here, we reappraise and update the increasing evidence for antibody-mediated immunity against Mycobacterium tuberculosis, discuss the complexity o...

  17. Viewing Translator as the Cultural Mediator from a Sociological Perspective

    Institute of Scientific and Technical Information of China (English)

    Chen Di

    2016-01-01

    Cultural differences, are different in their languages, their behaviour patterns, and values, therefore, an attempt to try to convey shared assumptions and responses using another language are unlikely to work. However, translator, being as the central role in the process of translation, functions as the cultural mediator through communicating and acting. The paper will mainly discuss translator as the cultural mediator incertain social context and explain it from a sociological perspective.

  18. Soluble mediators and the interaction of drugs in IBD

    DEFF Research Database (Denmark)

    Rask-Madsen, J

    1998-01-01

    , which provides the clinical manifestations of IBD. Other important soluble mediators of inflammation include complement-derived and chemotactic peptides, specific adhesion molecules, neuropeptides and reactive metabolites of oxygen and nitrogen. Current established therapies, such as glucocorticoids...... and 5-aminosalicylic acid (5-ASA), inhibit raised concentrations of these interdependent soluble mediators of inflammation, which may amplify one another or have parallel effects. Future medical options for treatment of IBD aim at removing perpetuating antigens or inhibiting the entry of inflammatory...

  19. Interpersonal aggression and burnout: the mediating role of psychological climate.

    Science.gov (United States)

    Bedi, Akanksha; Courcy, Francois; Paquet, Maxime; Harvey, Steve

    2013-12-01

    Using the theoretical perspectives offered by stressor-stress-strain framework and fairness theory, the authors propose that psychological climate will mediate the positive relationship between interpersonal aggression and employee burnout. Data from a survey of 1893 hospital employees suggested that psychological climate partially mediated the relationship between interpersonal aggression and two of the three dimensions of burnout, emotional exhaustion and depersonalization. The theoretical and practical implications of these findings and directions for future research are discussed.

  20. Robust and Adaptive MicroRNA-Mediated Incoherent Feedforward Motifs

    Institute of Scientific and Technical Information of China (English)

    XU Feng-Dan; LIU Zeng-Rong; ZHANG Zhi-Yong; SHEN Jian-Wei

    2009-01-01

    We integrate transcriptional and post-transcriptional regulation into microRNA-mediated incoherent feedforward motifs and analyse their dynamical behaviour and functions. The analysis show that the behaviour of the system is almost uninfluenced by the varying input in certain ranges and by introducing of delay and noise. The results indicate that microRNA-mediated incoherent feedforward motifs greatly enhance the robustness of gene regulation.