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Sample records for anorganickych soli ako

  1. SOLIS/VSM Polar Magnetic Field Data

    CERN Document Server

    Bertello, Luca

    2015-01-01

    The Vector Spectromagnetograph (VSM) instrument on the Synoptic Optical Long-term Investigations of the Sun (SOLIS) telescope is designed to obtain high-quality magnetic field observations in both the photosphere and chromosphere by measuring the Zeeman-induced polarization of spectral lines. With 1$^{\\prime \\prime}$ spatial resolution (1.14$^{\\prime \\prime}$ before 2010) and 0.05\\AA\\ spectral resolution, the VSM provides, among other products, chromospheric full-disk magnetograms using the CaII 854.2 nm spectral line and both photospheric full-disk vector and longitudinal magnetograms using the FeI 630.15 nm line. Here we describe the procedure used to compute daily weighted averages of the photospheric radial polar magnetic field at different latitude bands from SOLIS/VSM longitudinal full-disk observations. Time series of these measurements are publicly available from the SOLIS website at http://solis.nso.edu/0/vsm/vsm\\_plrfield.html. Future plans include the calculation of the mean polar field strength fr...

  2. 7. juunil kaitses Tallinna Ülikoolis oma doktoritööd Ako Sauga

    Index Scriptorium Estoniae

    2006-01-01

    Ako Sauga doktoritöö teema oli "Influence of environmental fluctuations on dynamics of non-linear systems" (pealkiri eesti keeles : "Keskkonnaparameetrite fluktuatsioonide mõju mittelineaarsete süsteemide dünaamikale")

  3. Inversions of High-Cadence SOLIS-VSM Stokes Observations

    NARCIS (Netherlands)

    Fischer, C.E.; Keller, C.U.; Snik, F.

    2008-01-01

    We have processed full-Stokes observations made with the SOLIS-VSM using Fe I 630.15 and Fe I 630.25 nm. The data have high spectral and temporal resolution, moderate spatial resolution, and large polarimetric sensitivity and accuracy. We use the code LILIA, an LTE inversion code written by Socas-Na

  4. Image Quality of SOLIS/VSM in Helium vs. Nitrogen

    OpenAIRE

    Harvey, J. W.

    2014-01-01

    The National Solar Observatory (NSO) Synoptic Optical Long-term Investigations of the Sun (SOLIS) Vector SpectroMagnetograph (VSM) is sealed and was designed to be filled with helium at slightly above ambient pressure. After 11 years of operation filled with helium, an acute shortage of helium prompted a test using nitrogen as the fill gas. Four months of nitrogen-filled observations in 2014 are compared the same months in 2013 with helium fill. On average, the image sharpness is slightly deg...

  5. Camera Gap Removal in SOLIS/VSM Images

    CERN Document Server

    Marble, Andrew R; Pevtsov, Alexei A

    2013-01-01

    The Vector Spectromagnetograph (VSM) instrument on the Synoptic Optical Longterm Investigations of the Sun (SOLIS) telescope is capable of obtaining spectropolarimetry for the full Sun (or a select latitudinal range) with one arcsecond spatial resolution and 0.05 Angstrom spectral resolution. This is achieved by scanning the Sun in declination and building up spectral cubes for multiple polarization states, utilizing a beamsplitter and two separate 2k x 2k CCD cameras. As a result, the eastern and western hemispheres of the Sun are separated in preliminary VSM images by a vertical gap with soft edges and variable position and width. Prior to the comprehensive analysis presented in this document, a trial-and-error approach to removing the gap had yielded an algorithm that was inconsistent, undocumented, and responsible for incorrectly eliminating too many image columns. Here we describe, in detail, the basis for a new, streamlined, and properly calibrated prescription for locating and removing the gap that is ...

  6. User experience network. Erroneous downstream occlusion alarms may disable Smiths Medical CADD-Solis infusion pumps.

    Science.gov (United States)

    2010-10-01

    Due to an issue in manufacturing, downstream occlusion (DSO) sensors in some Smiths Medical CADD-Solis infusion pumps may drift out of calibration, potentially resulting in erroneous alarms that disable the units. Hospitals experiencing the problem should return affected units to Smiths Medical for recalibration (free of charge) and should consider testing all their CADD-Solis pumps during routine maintenance to ensure that they alarm appropriately for downstream occlusions.

  7. Current Status of the SOLIS Program: Improved and New Data Products

    Science.gov (United States)

    Bertello, Luca; Britanik, John; Callahan, Lorraine; Gosain, Sanjay; Harker, Brian; Harvey, J. W.; Hughes, A.; Marble, A.; Pevtsov, Alexei A.; Wentzel, Thomas

    2016-05-01

    Over the past year the Synoptic Optical Long-term Investigations of the Sun (SOLIS) team has made significant improvements to the data products provided to the solar and heliospheric community. In particular, a considerable effort has been dedicated to reprocess the archive of vector and longitudinal photospheric magnetograms, from 2003 to present, using the latest production code. This endeavor is now near completion and will assure that all derived magnetic products, such as synoptic maps and flux time series, are consistently calibrated. In addition, new products have been recently developed. For example, time series of daily averages of the Sun's polar magnetic field derived from full-disk photospheric FeI 630.15 nm longitudinal magnetograms are now available from the SOLIS website at solis.nso.edu/0/vsm/vsm_plrfield.html.New intensity calibration of high resolution Ca II K & H spectra from the Integrated Sunlight Spectrometer (ISS) led to significant reduction in daily variations of parameters derived from these spectra. The SOLIS team will soon also release Carrington synoptic maps for the three components of the photospheric magnetic field derived from vector measurements taken in the Fe I 630.15 nm spectral line. The addition, in late 2015, of a new Ca II 854.2 nm full-Stokes polarimeter into the SOLIS core program of magnetic observations will make available to the community daily chromospheric measurements of the complete Stokes polarization vector.We present here a summary of these improvements, with particular emphasis on the new products that can be accessed from the SOLIS data page at solis.nso.edu/0/solis_data.html. For a description of the diagnostic capability of the new Ca II 854.2 nm spectro-polarimeter and preliminary results we refer to other presentations by SOLIS team members at this meeting.This work utilizes SOLIS data obtained by the NSO Integrated Synoptic Program (NISP), managed by the National Solar Observatory, which is operated by the

  8. Virgibacillus soli sp. nov., isolated from mountain soil.

    Science.gov (United States)

    Kämpfer, P; Arun, A B; Busse, H-J; Langer, S; Young, C-C; Chen, W-M; Syed, A A; Rekha, P D

    2011-02-01

    A Gram-positive bacterium (strain CC-YMP-6(T)) was isolated from soil samples collected from Yang-Ming Mountain, Taiwan. On the basis of 16S rRNA gene sequence analysis, strain CC-YMP-6(T) clearly belonged to the genus Virgibacillus and was most closely related to the type strains of Virgibacillus halophilus (96.2 % similarity) and Virgibacillus kekensis (96.3 %). The predominant isoprenoid quinone was menaquinone MK-7 and the polar lipid profile was composed of the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid plus moderate amounts of two unidentified aminophospholipids and a phospholipid. The polyamine pattern comprised spermidine as the single major component with spermine and putrescine present in minor amounts. The major fatty acids of strain CC-YMP-6(T) were iso-C(15 : 0) and anteiso-C(15 : 0). The results of physiological and biochemical tests allowed the clear phenotypic differentiation of strain CC-YMP-6(T) from all recognized species of the genus Virgibacillus. Strain CC-YMP-6(T) is therefore considered to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus soli sp. nov. is proposed. The type strain is CC-YMP-6(T) (=DSM 22952(T)=CCM 7714(T)). PMID:20228217

  9. Luteimonas soli sp. nov., isolated from farmland soil.

    Science.gov (United States)

    Wang, Xiang; Yang, Hong-Xing; Zhang, Ying-Kun; Zhu, Shi-Jun; Liu, Xiao-Wei; Zhang, Hao; Zhang, Chen-Fei; Zhao, Chao-Ran; Hu, Gang; Hong, Qing

    2015-12-01

    A yellow-pigmented bacterial strain, designated Y2T, was isolated from farmland soil in Bengbu, Anhui province, China. Cells of strain Y2T were Gram-stain-negative, strictly aerobic, non-motile and rod-shaped. Strain Y2T grew optimally at pH 7.0, 30 °C and in the presence of 2 % (w/v) NaCl. The DNA G+C content was 68.9 mol%. The major fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0, summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1ω9c), iso-C11 : 0 3-OH and iso-C11 : 0. The major respiratory quinone was ubiquinone-8 (Q-8), and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain Y2T was most closely related to Luteimonas mephitis B1953/27.1T (99.1 % 16S rRNA gene sequence similarity), followed by Luteimonas lutimaris G3T (98.6 %), Luteimonas abyssi XH031T (96.2 %) and Luteimonas aquatica RIB1-20T (96.0 %). Strain Y2T exhibited low DNA-DNA relatedness with Luteimonas mephitis B1953/27.1T (43.6 ± 0.5 %) and Luteimonas lutimaris G3T (43.9 ± 2.1 %). On the basis of phenotypic, genotypic and phylogenetic evidence, strain Y2T represents a novel species of the genus Luteimonas, for which the name Luteimonas soli sp. nov. is proposed. The type strain is Y2T ( = ACCC 19799T = KCTC 42441T). PMID:26437920

  10. Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer Tissue

    OpenAIRE

    Saskia Biskup; Detlef Boehm; Diana Boehm; Falko Fend; Martin Braun; Mario Deng; Roopika Menon; Sven Perner

    2012-01-01

    Next generation sequencing (NGS) technologies have revolutionized cancer research allowing the comprehensive study of cancer using high throughput deep sequencing methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation and Detection, Life Technologies) is a promising technology generating billions of 50 bp sequencing reads. This robust technique, successfully applied in ...

  11. Vector magnetic field inversions of high cadence SOLIS-VSM data

    NARCIS (Netherlands)

    Fischer, C.E.; Keller, C.U.; Snik, F.

    2007-01-01

    We have processed full Stokes observations from the SOLIS VSM in the photospheric lines Fe I 630.15 nm and 630.25 nm. The data sets have high spectral and temporal resolution, moderate spatial resolution, and large polarimetric sensitivity and accuracy. We used the LILIA, an LTE code written by Soca

  12. Hybrid De Novo Genome Assembly Using MiSeq and SOLiD Short Read Data.

    Directory of Open Access Journals (Sweden)

    Tsutomu Ikegami

    Full Text Available A hybrid de novo assembly pipeline was constructed to utilize both MiSeq and SOLiD short read data in combination in the assembly. The short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components such as ABySS and SOAPdenovo. The assembly pipeline proceeded through several stages, and either MiSeq paired-end data, SOLiD mate-paired data, or both of them could be specified as input data at each stage separately. The pipeline was examined on the filamentous fungus Aspergillus oryzae RIB40, by aligning the assembly results against the reference sequences. Using both the MiSeq and the SOLiD data in the hybrid assembly, the alignment length was improved by a factor of 3 to 8, compared with the assemblies using either one of the data types. The number of the reproduced gene cluster regions encoding secondary metabolite biosyntheses (SMB was also improved by the hybrid assemblies. These results imply that the MiSeq data with long read length are essential to construct accurate nucleotide sequences, while the SOLiD mate-paired reads with long insertion length enhance long-range arrangements of the sequences. The pipeline was also tested on the actinomycete Streptomyces avermitilis MA-4680, whose gene is known to have high-GC content. Although the quality of the SOLiD reads was too low to perform any meaningful assemblies by themselves, the alignment length to the reference was improved by a factor of 2, compared with the assembly using only the MiSeq data.

  13. 美国第一位头颈外科医师Jacob Da Silva Solis-Cohen

    Institute of Scientific and Technical Information of China (English)

    王荣光

    2010-01-01

    @@ Jacob Da Silva Solis.Cohen(1838~1927)是19世纪末20世纪初杰出的内科和外科医师,他的从医生涯记录了喉科学和头颈外科学的起源与发展. 1838年2月29日Jacob Da Silva Solis-Cohen出生于美国纽约,他的先辈在犹太区非常知名,在北美革命期间是著名的慈善家和爱国者,1840年Solis-Cohen全家移居费城.Solis-Cohen在费城完成了大学教育,随后在Jefferson医学院学习,1860年他在Pennsylvania大学完成医学教育后在费城医院担任了6个月的住院医师.

  14. Status of the Synoptic Optical Long-Term Investigations of the Sun (SOLIS) Facility

    Science.gov (United States)

    Harvey, J.; SOLIS Team

    2003-05-01

    SOLIS is a suite of three instruments and a data handling system designed to provide synoptic solar observations for at least two decades. The facility is now commencing initial operations at a temporary observing site in Tucson. After a period of simultaneous observations with the NSO/NASA Kitt Peak spectromagnetograph, SOLIS will be moved to Kitt Peak where it will replace that instrument and the 30-year-old Vacuum Telescope. The SOLIS Vector SpectroMagnetograph (VSM) is a compact, helium-filled, high-throughput, 50-cm aperture telescope and spectrograph that nominally scans the entire solar disk in 15 minutes. It provides the first full-disk photospheric vector magnetograms, high-sensitivity photospheric and chromospheric longitudinal magnetograms, and 1083 nm He I chromospheric spectral maps. The VSM is initially operating with interim cameras that are noisier and slower than originally planned. A 15 cm Full Disk Patrol (FDP) instrument provides full-disk filtergrams captured by 2k x 2k CCD cameras using either a 1083 nm or a tunable (380-660 nm) narrow-band filter. Until it is finished, the tunable filter is temporarily replaced by an H-alpha filter. The VSM and FDP are mounted on separate declination axes attached to a right ascension drive. This allows the FDP to be pointed at the sun center while the VSM scans the solar disk in the declination direction. The final instrument is an Integrated Sunlight Spectrometer (ISS). The ISS is a double-pass spectrograph that produces high-quality spectra for sun-as-a-star studies. It is located in a temperature-controlled room and fed by a fiber-optic image-scrambling system that is mounted on the FDP. Data from all of the instruments is collected by an on-site storage area network and preliminary reduction done by a collection of 14 Linux computers. Archival storage of the data is on LTO tapes and the data products are transmitted to Tucson via a 45 Mbs link where they are kept on line and web-available using a RAID 5

  15. Fine De Novo Sequencing of a Fungal Genome Using only SOLiD Short Read Data: Verification on Aspergillus oryzae RIB40

    OpenAIRE

    Myco Umemura; Yoshinori Koyama; Itaru Takeda; Hiroko Hagiwara; Tsutomu Ikegami; Hideaki Koike; Masayuki Machida

    2013-01-01

    The development of next-generation sequencing (NGS) technologies has dramatically increased the throughput, speed, and efficiency of genome sequencing. The short read data generated from NGS platforms, such as SOLiD and Illumina, are quite useful for mapping analysis. However, the SOLiD read data with lengths of

  16. Draft Genome Sequence of a Multidrug-Resistant blaNDM-1-Producing Acinetobacter soli Isolate in China

    OpenAIRE

    Chen, Yan; Yan, Zhengsong; Wang, Miaojuan; Zheng, Xia; Lu, Yun; Lin, Shaohua

    2014-01-01

    Acinetobacter spp. are one of the most prevalent opportunistic pathogens causing nosocomial infections and have become a major clinical and public health threat. In this study, we presented the first draft genome sequence of A. soli TCM341, a multidrug resistant isolate that carried the blaNDM-1 gene in China. Genome sequencing of A. soli TCM341 was carried out in Illumina Hiseq 2000 next-generation sequencer. The data obtained revealed 74 contigs with genome size of 3.49 Mb and G+C content o...

  17. Zeemanfit: Use and Development of the solis_vms_zeemanfit code

    CERN Document Server

    Hughes, Anna L H; Marble, Andrew R; Pevtsov, Alexei A

    2013-01-01

    The purpose of the SOLIS Zeemanfit Code is to provide a straight-forward, easily checked measure of the total magnetic-field strength in the high-strength umbral regions of the solar disk. In the highest-strength regions, the Zeeman splitting of the 6302-angstrom Fe line becomes wide enough for the triplet nature of the line to be visible by eye in non-polarized light. Therefore, a three-line fit to the spectra should, in principle, provide a fairly robust measure of the total magnetic-field strength. The code uses the Level-1.5 spec-cube data of the SOLIS VSM 6302-vector observations (specifically the Stokes-I and Stokes-V components) to fit the line profiles at each appropriate pixel and calculate the magnetic-field-strength from the line-center separation of the two fit 6302.5 sigma-components. The 6301.5-angstrom Fe line is also present and fit in the VSM 6302-vector data, but it is an anomalous-Zeeman line with a weaker response to magnetic fields. Therefore, no magnetic- field measure is derived from th...

  18. Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE Prostate Cancer Tissue

    Directory of Open Access Journals (Sweden)

    Saskia Biskup

    2012-07-01

    Full Text Available Next generation sequencing (NGS technologies have revolutionized cancer research allowing the comprehensive study of cancer using high throughput deep sequencing methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation and Detection, Life Technologies is a promising technology generating billions of 50 bp sequencing reads. This robust technique, successfully applied in gene identification, might be helpful in detecting novel genes associated with cancer initiation and progression using formalin fixed paraffin embedded (FFPE tissue. This study’s aim was to compare the validity of whole exome sequencing of fresh-frozen vs. FFPE tumor tissue by normalization to normal prostatic FFPE tissue, obtained from the same patient. One primary fresh-frozen sample, corresponding FFPE prostate cancer sample and matched adjacent normal prostatic tissue was subjected to exome sequencing. The sequenced reads were mapped and compared. Our study was the first to show comparable exome sequencing results between FFPE and corresponding fresh-frozen cancer tissues using SOLiD sequencing. A prior study has been conducted comparing the validity of sequencing of FFPE vs. fresh frozen samples using other NGS platforms. Our validation further proves that FFPE material is a reliable source of material for whole exome sequencing.

  19. Morphology of Wrinkle Ridges on Lunae and Solis Plana, Mars from MOLA Topography: Implications for Their Kinematic Development

    Science.gov (United States)

    Tate, A.; Golombek, M. P.; Mueller, K. J.

    2001-01-01

    Mars Orbiter Laser Altimeter (MOLA) topography is used to define the detailed morphology and kinematic development of martian wrinkle ridges on Lunae and Solis Plana. Changes in ridge morphology suggest they form as fault-propagation folds, often with significant backthrusts. Additional information is contained in the original extended abstract.

  20. SOLIS: reconciling disk-integrated and disk-resolved spectra from the Sun

    CERN Document Server

    Pevtsov, Alexei; Harker, Brian; Giampapa, Mark; Marble, Andrew

    2014-01-01

    Unlike other stars, the surface of the Sun can be spatially resolved to a high degree of detail. But the Sun can also be observed as if it was a distant star. The availability of solar disk-resolved and disk-integrated spectra offers an opportunity to devise methods to derive information about the spatial distribution of solar features from Sun-as-a-star measurements. Here, we present an update on work done at the National Solar Observatory to reconcile disk-integrated and disk-resolved solar spectra from the Synoptic Optical Long-term Investigation of the Sun (SOLIS) station. The results of this work will lead to a new approach to infer the information about the spatial distribution of features on other stars, from the overall filling factor of active regions to, possibly, the latitude/longitude distribution of features.

  1. Automatic Active-Region Identification and Azimuth Disambiguation of the SOLIS/VSM Full-Disk Vector Magnetograms

    CERN Document Server

    Georgoulis, M K; Henney, C J

    2007-01-01

    The Vector Spectromagnetograph (VSM) of the NSO's Synoptic Optical Long-Term Investigations of the Sun (SOLIS) facility is now operational and obtains the first-ever vector magnetic field measurements of the entire visible solar hemisphere. To fully exploit the unprecedented SOLIS/VSM data, however, one must first address two critical problems: first, the study of solar active regions requires an automatic, physically intuitive, technique for active-region identification in the solar disk. Second, use of active-region vector magnetograms requires removal of the azimuthal $180^o$-ambiguity in the orientation of the transverse magnetic field component. Here we report on an effort to address both problems simultaneously and efficiently. To identify solar active regions we apply an algorithm designed to locate complex, flux-balanced, magnetic structures with a dominant E-W orientation on the disk. Each of the disk portions corresponding to active regions is thereafter extracted and subjected to the Nonpotential M...

  2. Depth and Morphology of Wrinkle Ridge Detachments at Solis Planum, Mars

    Science.gov (United States)

    Colton, S. L.; Ferrill, D. A.; Smart, K. J.

    2005-12-01

    Wrinkle ridges -- long, linear to sinuous anticlines separated by relatively broad, flat synclinal valleys -- are a fundamental component of Martian geomorphology. The anticlinal crests show variable morphologies, but are often characterized by weak to strong asymmetry with variable vergence directions between adjacent ridges and along strike for any given ridge. Although wrinkle ridges are typically interpreted as contractional features, there is ongoing debate about their underlying structure and whether thrust faults penetrate to tens of kilometers of depth ("thick-skinned shortening") or sole into a detachment in the upper few kilometers of the Martian crust ("thin-skinned shortening"). Previous workers have estimated depth to the detachment horizon using a variety of methods including gravity inversion, geometry of crater-ridge intersections, mechanical modeling, and geometric modeling. Here we use a well-established terrestrial technique to calculate depth to the detachment horizon for wrinkle ridges in the Solis Planum region of Mars. We interpolate topographic profiles perpendicular to the regional trend of wrinkle ridges from Mars Orbiter Laser Altimeter (MOLA) Mission Experiment Gridded Data Record (MEGDR) altimetry data, set vertical reference lines on both sides of the ridge that define the limits of our measurement range, estimate the topographic surface prior to wrinkle ridge formation, and calculate the area uplifted above the original topographic surface. Dividing this excess area by the amount of shortening (the topographic profile length minus the length prior to deformation), provides depth to detachment. We calibrate the results with profiles from the less spatially-extensive but greater along-track density MOLA Precision Experiment Data Record (PEDR). Additional topographic and structural interpretation and analysis of wrinkle ridge morphology are conducted with Mars Orbiter Camera (MOC), Thermal Emission Imaging System (THEMIS), and High

  3. Deinococcus soli sp. nov., a gamma-radiation-resistant bacterium isolated from rice field soil.

    Science.gov (United States)

    Cha, Seho; Srinivasan, Sathiyaraj; Seo, Taegun; Kim, Myung Kyum

    2014-06-01

    A Gram-negative, non-motile, short rod-shaped bacterial strain, designated N5(T), was isolated from a rice field soil in South Korea. Phylogenetic analysis based on the 16S rRNA gene sequence of the new isolate showed that strain N5(T) belongs to the genus Deinococcus, family Deinococcaceae, showing the highest sequence similarity to Deinococcus grandis KACC 11979(T) (98.4 %) and Deinococcus daejeonensis KCTC 13751(T) (97.5 %). Strain N5(T) exhibits resistance to gamma-radiation similar to that of other members of the genus Deinococcus, with a D10 value in excess of 4 kGy. Chemotaxonomic data showed that the most abundant fatty acids are C16:1ω7c (25.25 %), C15:1ω6c (19.77 %), C17:1ω6c (11.87 %), and C17:0 (9.41 %), and the major polar lipid is an unknown phosphoglycolipid. The predominant respiratory quinone is menaquinone MK-8. The DNA G+C content is 71.4 mol%. Phenotypic, phylogenetic, and chemotaxonomic data support designation of strain N5(T) as a novel species of the genus Deinococcus, for which the name Deinococcus soli sp. nov. is proposed. The type strain is N5(T) (=KCTC 33153(T) = JCM 19176(T)).

  4. Rheinheimera soli sp. nov., a gammaproteobacterium isolated from soil in Korea.

    Science.gov (United States)

    Ryu, Seung Hyun; Chung, Bok Sil; Park, Minjeong; Lee, Seung Sik; Lee, Sang-Suk; Jeon, Che Ok

    2008-10-01

    A Gram-negative, non-spore-forming bacterium, designated strain BD-d46(T), was isolated from a playground soil sample in Jinju, South Korea. Cells were straight or curved rods and showed catalase- and oxidase-positive reactions. Growth of strain BD-d46(T) was observed between 15 and 35 degrees C (optimum 25-30 degrees C) and between pH 6.5 and 8.0 (optimum pH 7.0-7.5). The predominant fatty acids were summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), C(12 : 0) 3-OH and C(16 : 0). Strain BD-d46(T) contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Isoprenoid quinones were Q-8 (75 %) and MK-7 (25 %). The G+C content of the genomic DNA was 49.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-d46(T) formed a distinct lineage with Rheinheimera chironomi K19414(T) within the genus Rheinheimera. Levels of 16S rRNA gene sequence similarity between strain BD-d46(T) and the type strains of recognized Rheinheimera species ranged from 94.4 to 96.9 %. On the basis of chemotaxonomic data and molecular properties, strain BD-d46(T) is considered to represent a novel species of the genus Rheinheimera, for which the name Rheinheimera soli sp. nov. is proposed. The type strain is BD-d46(T) (=KCTC 22077(T) =DSM 19413(T)).

  5. Complete genome sequence of Deinococcus soli N5(T), a gamma-radiation- resistant bacterium isolated from rice field in South Korea.

    Science.gov (United States)

    Joo, Eun Sun; Kim, Eun Bit; Jeon, Seon Hwa; Srinivasan, Sathiyaraj

    2015-10-10

    A Gram-negative, non-motile and short-rod shaped and gamma-radiation-resistant bacterium Deinococcus soli N5(T), isolated from a rice field soil in South Korea. The complete genome of D. soli N5(T) consists of a chromosome (3,236,984bp). The key enzymes for the central DNA repair mechanisms were present in the genome. The enzyme coding genes has been identified which is involving in the nucleotide excision repair (NER) pathway. The gene cluster in the genome sequence suggest that the D. soli N5(T) use (NER) pathways for efficient removal of pyrimidine dimers that are the most abundant type of UV- induced damage.

  6. Fine de novo sequencing of a fungal genome using only SOLiD short read data: verification on Aspergillus oryzae RIB40.

    Directory of Open Access Journals (Sweden)

    Myco Umemura

    Full Text Available The development of next-generation sequencing (NGS technologies has dramatically increased the throughput, speed, and efficiency of genome sequencing. The short read data generated from NGS platforms, such as SOLiD and Illumina, are quite useful for mapping analysis. However, the SOLiD read data with lengths of <60 bp have been considered to be too short for de novo genome sequencing. Here, to investigate whether de novo sequencing of fungal genomes is possible using only SOLiD short read sequence data, we performed de novo assembly of the Aspergillus oryzae RIB40 genome using only SOLiD read data of 50 bp generated from mate-paired libraries with 2.8- or 1.9-kb insert sizes. The assembled scaffolds showed an N50 value of 1.6 Mb, a 22-fold increase than those obtained using only SOLiD short read in other published reports. In addition, almost 99% of the reference genome was accurately aligned by the assembled scaffold fragments in long lengths. The sequences of secondary metabolite biosynthetic genes and clusters, whose products are of considerable interest in fungal studies due to their potential medicinal, agricultural, and cosmetic properties, were also highly reconstructed in the assembled scaffolds. Based on these findings, we concluded that de novo genome sequencing using only SOLiD short reads is feasible and practical for molecular biological study of fungi. We also investigated the effect of filtering low quality data, library insert size, and k-mer size on the assembly performance, and recommend for the assembly use of mild filtered read data where the N50 was not so degraded and the library has an insert size of ∼2.0 kb, and k-mer size 33.

  7. Axial Surface Mapping of Wrinkle Ridges on Solis Planum, Mars from MOLA Topography: Constraints on Subsurface Blind Thrust Geometry

    Science.gov (United States)

    Vidal, A.; Mueller, K.; Golombek, M. P.

    2003-01-01

    We undertook axial surface mapping of selected wrinkle ridges on Solis Planum, Mars in order to assess the subsurface geometry of blind thrusts proposed to exist beneath them. This work builds on previous work that defined structural families of wrinkle ridges based on their surface morphology in this region. Although a growing consensus exists for models of wrinkle ridge kinematics and mechanics, a number of current problems remain. These include the origin of topographic offset across the edges of wrinkle ridges, the relationship between broad arches and superposed ridges, the origin of smaller wrinkles, and perhaps most importantly, the trajectory of blind thrusts that underlie wrinkle ridges and accommodate shortening at deeper crustal levels. We are particularly interested in defining the depths at which blind thrusts flatten under wrinkle ridges in order to provide constraints on the brittle-ductile transition during Early Hesperian time. We also seek to test whether wrinkle ridges on Solis Planum develop above reactivated faults or newly formed ones.

  8. Genome sequence of a dissimilatory Fe(III)-reducing bacterium Geobacter soli type strain GSS01(T).

    Science.gov (United States)

    Yang, Guiqin; Chen, Shanshan; Zhou, Shungui; Liu, Yongfeng

    2015-01-01

    Strain GSS01(T) (=KCTC 4545=MCCC 1 K00269) is the type strain of the species Geobacter soli. G. soli strain GSS01(T) is of interest due to its ability to reduce insoluble Fe(III) oxides with a wide range of electron donors. Here we describe some key features of this strain, together with the whole genome sequence and annotation. The genome of size 3,657,100 bp contains 3229 protein-coding and 54 RNA genes, including 2 16S rRNA genes. The genome of strain GSS01(T)contains 76 predicted cytochrome genes, 24 pilus assembly protein genes and several other genes, which were proposed to be related to the reduction of insoluble Fe(III) oxides. The genes associated with the electron donors and acceptors of strain GSS01(T) were predicted in the genome. Information gained from its sequence will be relevant to the future elucidation of extracellular electron transfer mechanism during the reduction of Fe(III) oxides. PMID:26634019

  9. Decoding the Hermetic Discourse in Salomon Trismosin's Splendor Solis - A Semiotic Study of Three Ways of Reading

    Directory of Open Access Journals (Sweden)

    J. Peter Södergård

    1996-01-01

    Full Text Available Alchemy and the Hermetic Art are terms that denote a most interesting transitional space, circumscribing an opaque region of human cultural history, shared between matter and psyche, between phantasmagoric reveries and practical experiments, between sincere natural theology and conscious fraud. This is an area of human experience that has been notoriously difficult to define and understand, and which has triggered off contradictory interpretations that are, in their own, of semiotic interest. It has been said that a text — in our case Splendor Solis, and the Hermetic–alchemical texts at large — is a picnic, a Dutch party, in which the author provides the words and the reader, especially a reader with too much or too little erudition, comes up with the meaning of the words. In this article, the author tries to give an overview of the general interpretations of alchemy that have been put forward, and apply Umberto Eco's intentional approach to these interpretations, viz. consider them as various sorts of intentions. The author uses a pseudonymous alchemical tractate from the sixteenth century, Salomon Trismosin's treatise Splendor Solis as an opportunity to picture and evaluate the three main decodings of alchemy that have been attempted; a chemical, a religious—soteriological and a psychological. The author also explores the intertextual web in the Hermetic discourse, and see if its mapping can generate usable knowledge about what has, since the Middle Ages, been called alchemy: a term acknowledging the taking up of a science or art which had been cultivated by Muslim and Nestorian scholars, but with its fountain head in Late Antiquity.

  10. Two new species of dicyemid mesozoans (Dicyemida: Dicyemidae) from Octopus maya Voss & Solis-Ramirez (Octopodidae) off Yucatan, Mexico.

    Science.gov (United States)

    Castellanos-Martinez, Sheila; Aguirre-Macedo, M Leopoldina; Furuya, Hidetaka

    2016-07-01

    Two new dicyemid species are described from the endemic cephalopod Octopus maya Voss & Solis-Ramirez collected off Yucatan, Mexico. The renal sacs of 40 juvenile and adult octopuses from four localities were examined. Dicyema hochbergi n. sp. is a medium-sized species that reaches 2,245 µm in length. The vermiform stages consist of 18-24 peripheral cells, a conical calotte and the extension of the axial cell between the base and middle of the metapolar cells. Infusoriform embryos consist of 39 cells with urn cell containing one germinal cell, two nuclei and solid refringent bodies. Dicyema mexcayae n. sp. is a relatively small species that reaches 1,114 µm in length. The vermiform stages are constituted by 14-16 peripheral cells, an elongate calotte and the axial cell extending forward to the middle of the metapolar cells. The infusoriform embryos consist of 37 cells, two solid refringent bodies and urn cells with two nuclei each. The present study represents the first description of a dicyemid species from O. maya and increases the number of described species from Mexican waters to 11. PMID:27307168

  11. Two new species of dicyemid mesozoans (Dicyemida: Dicyemidae) from Octopus maya Voss & Solis-Ramirez (Octopodidae) off Yucatan, Mexico.

    Science.gov (United States)

    Castellanos-Martinez, Sheila; Aguirre-Macedo, M Leopoldina; Furuya, Hidetaka

    2016-07-01

    Two new dicyemid species are described from the endemic cephalopod Octopus maya Voss & Solis-Ramirez collected off Yucatan, Mexico. The renal sacs of 40 juvenile and adult octopuses from four localities were examined. Dicyema hochbergi n. sp. is a medium-sized species that reaches 2,245 µm in length. The vermiform stages consist of 18-24 peripheral cells, a conical calotte and the extension of the axial cell between the base and middle of the metapolar cells. Infusoriform embryos consist of 39 cells with urn cell containing one germinal cell, two nuclei and solid refringent bodies. Dicyema mexcayae n. sp. is a relatively small species that reaches 1,114 µm in length. The vermiform stages are constituted by 14-16 peripheral cells, an elongate calotte and the axial cell extending forward to the middle of the metapolar cells. The infusoriform embryos consist of 37 cells, two solid refringent bodies and urn cells with two nuclei each. The present study represents the first description of a dicyemid species from O. maya and increases the number of described species from Mexican waters to 11.

  12. Optimization of Bartonella henselae multilocus sequence typing scheme using single-nucleotide polymorphism analysis of SOLiD sequence data

    Institute of Scientific and Technical Information of China (English)

    ZHAO Fan; Gemma Chaloner; Alistair Darby; SONG Xiu-ping; LI Dong-mei; Richard Birtles; LIU Qi-yong

    2012-01-01

    Background Multi-locus sequence typing (MLST) is widely used to explore the population structure of numerous bacterial pathogens.However,for genotypically-restricted pathogens,the sensitivity of MLST is limited by a paucity of variation within selected loci.For Bartonella henselae (B.henselae),although the MLST scheme currently used has been proven useful in defining the overall population structure of the species,its reliability for the accurate delineation of closely-related sequence types,between which allelic variation is usually limited to,at most,one or two nucleotide polymorphisms.Exploitation of high-throughput sequencing data allows a more informed selection of MLST loci and thus,potentially,a means of enhancing the sensitivity of the schemes they comprise.Methods We carried out SOLiD resequencing on 12 representative B.henselae isolates and explored these data using single nucleotide polymorphism (SNP) analysis.We determined the number and distribution of SNPs in the genes targeted by the established MLST scheme and modified the position of loci within these genes to capture as much genetic variation as possible.Results Using genome-wide SNP data,we found the distribution of SNPs within each open reading frame (ORF) of MLST loci,which were not represented by the established B.henselae MLST scheme.We then modified the position of loci in the MLST scheme to better reflect the polymorphism in the ORF as a whole.The use of amended loci in this scheme allowed previously indistinguishable ST1 strains to be differentiated.However,the diversity of B.henselae was still rare in China.Conclusions Our study demonstrates the use of SNP analysis to facilitate the selection of MLST loci to augment the currently-described scheme for B.henselae.And the diversity among B.henselae strains in China is markedly less than that observed in B.henselae populations elsewhere in the world.

  13. Transcription profiling of the model cyanobacterium Synechococcus sp. strain PCC 7002 by NextGen (SOLiD™ Sequencing of cDNA

    Directory of Open Access Journals (Sweden)

    Marcus eLudwig

    2011-03-01

    Full Text Available The genome of the unicellular, euryhaline cyanobacterium Synechococcus sp. PCC 7002 encodes about 3200 proteins. Transcripts were detected for nearly all annotated open reading frames by a global transcriptomic analysis by Next-Generation (SOLiDTM sequencing of cDNA. In the cDNA samples sequenced, ~90% of the mapped sequences were derived from the 16S and 23S ribosomal RNAs and ~10% of the sequences were derived from mRNAs. In cells grown photoautotrophically under standard conditions (38 °C, 1% (v/v CO2 in air, 250 µmol photons m-2 s-1, the highest transcript levels (up to 2% of the total mRNA for the most abundantly transcribed genes (e. g., cpcAB, psbA, psaA were generally derived from genes encoding structural components of the photosynthetic apparatus. High light exposure for one hour caused changes in transcript levels for genes encoding proteins of the photosynthetic apparatus, Type-1 NADH dehydrogenase complex and ATP synthase, whereas dark incubation for one hour resulted in a global decrease in transcript levels for photosynthesis-related genes and an increase in transcript levels for genes involved in carbohydrate degradation. Transcript levels for pyruvate kinase and the pyruvate dehydrogenase complex decreased sharply in cells incubated in the dark. Under dark anoxic (fermentative conditions, transcript changes indicated a global decrease in transcripts for respiratory proteins and suggested that cells employ an alternative phosphoenolpyruvate degradation pathway via phosphoenolpyruvate synthase (ppsA and the pyruvate:ferredoxin oxidoreductase (nifJ. Finally, the data suggested that an apparent operon involved in tetrapyrrole biosynthesis and fatty acid desaturation, acsF2-ho2-hemN2-desF, may be regulated by oxygen concentration.

  14. SOLiD™ sequencing of genomes of clinical isolates of Leishmania donovani from India confirm leptomonas co-infection and raise some key questions.

    Directory of Open Access Journals (Sweden)

    Neeloo Singh

    Full Text Available BACKGROUND: Known as 'neglected disease' because relatively little effort has been applied to finding cures, leishmaniasis kills more than 150,000 people every year and debilitates millions more. Visceral leishmaniasis (VL, also called Kala Azar (KA or black fever in India, claims around 20,000 lives every year. Whole genome analysis presents an excellent means to identify new targets for drugs, vaccine and diagnostics development, and also provide an avenue into the biological basis of parasite virulence in the L. donovani complex prevalent in India. METHODOLOGY/PRINCIPAL FINDINGS: In our presently described study, the next generation SOLiD™ platform was successfully utilized for the first time to carry out whole genome sequencing of L. donovani clinical isolates from India. We report the exceptional occurrence of insect trypanosomatids in clinical cases of visceral leishmaniasis (Kala Azar patients in India. We confirm with whole genome sequencing analysis data that isolates which were sequenced from Kala Azar (visceral leishmaniasis cases were genetically related to Leptomonas. The co-infection in splenic aspirate of these patients with a species of Leptomonas and how likely is it that the infection might be pathogenic, are key questions which need to be investigated. We discuss our results in the context of some important probable hypothesis in this article. CONCLUSIONS/SIGNIFICANCE: Our intriguing results of unusual cases of Kala Azar found to be most similar to Leptomonas species put forth important clinical implications for the treatment of Kala Azar in India. Leptomonas have been shown to be highly susceptible to several standard leishmaniacides in vitro. There is very little divergence among these two species viz. Leishmania sp. and L. seymouri, in terms of genomic sequence and organization. A more extensive perception of the phenomenon of co-infection needs to be addressed from molecular pathogenesis and eco

  15. High prevalence of blaOXA-23 in Acinetobacter spp. and detection of blaNDM-1 in A. soli in Cuba: report from National Surveillance Program (2010–2012

    Directory of Open Access Journals (Sweden)

    D. Quiñones

    2015-09-01

    Full Text Available As a first national surveillance of Acinetobacter in Cuba, a total of 500 Acinetobacter spp. isolates recovered from 30 hospitals between 2010 and 2012 were studied. Acinetobacter baumannii–calcoaceticus complex accounted for 96.4% of all the Acinetobacter isolates, while other species were detected at low frequency (A. junii 1.6%, A. lwoffii 1%, A. haemolyticus 0.8%, A. soli 0.2%. Resistance rates of isolates were 34–61% to third-generation cephalosporins, 49–50% to β-lactams/inhibitor combinations, 42–47% to aminoglycosides, 42–44% to carbapenems and 55% to ciprofloxacin. However, resistance rates to colistin, doxycycline, tetracycline and rifampin were less than 5%. Among carbapenem-resistant isolates, 75% harboured different blaOXA genes (OXA-23, 73%; OXA-24, 18%; OXA-58, 3%. The blaNDM-1 gene was identified in an A. soli strain, of which the species was confirmed by sequence analysis of 16S rRNA gene, rpoB, rpoB–rpoC and rpoL–rpoB intergenic spacer regions and gyrB. The sequences of blaNDM-1 and its surrounding genes were identical to those reported for plasmids of A. baumannii and A. lwoffi strains. This is the first report of blaNDM-1 in A. soli, together with a high prevalence of OXA-23 carbapenemase for carbapenem resistance in Acinetobacter spp. in Cuba.

  16. Renovation of Shaft Mining Building No. 2 in Kłodawa Salt Mine/ Renowacja Budynku Nadszybia Nr 2 Na Terenie Kopalni Soli "Kłodawa" S.A.

    Science.gov (United States)

    Błaszczyński, Tomasz; Wielentejczyk, Przemysław

    2015-06-01

    The paper presents the renovation process of the shaft mining building No. 2 situated in the Kłodawa Salt Mine. A technical state of the facility required immediate reinforcement of structural elements, which was confirmed by expertise carried out by the authors. A lack of repairs could be the cause of building damage. The progress of corrosion in some steel profiles of columns or floors was very advanced. The state of the building was rapidly worsening due to the very high salinity of the indoor environment, moisture (building not insulated) and vibrating engines of machinery operating on different floors felt throughout the facility. After carrying out the technical expertise, working plans and specifications, and relevant numerical analysis, the modernization process was realized by the reinforcement or rebuilding of structural elements. Referat przedstawia sposób remontu i naprawy konstrukcji nośnej i obudowy budynku nadszybia nr 2 na terenie Kopalni Soli "Kłodawa". Elementy konstrukcyjne budynku wymagały natychmiastowego wzmocnienia, co potwierdziła ekspertyza wykonana przez autorów. Zaniechanie prac remontowych groziło awarią budowlaną pomimo przeprowadzonej wcześniej w 2002 r. naprawy. Postęp korozji w niektórych profilach stalowych słupów czy stropów był bardzo zaawansowany. Stan obiektu pogarszał się szybko ze względu na bardzo duże zasolenie środowiska, wilgoć (budynek nieocieplony) oraz pracujące na poszczególnych poziomach maszyny wytwarzające duże drgania wyczuwalne w każdym miejscu obiektu. Wykonano ekspertyzę i projekt wykonawczy przed przystąpieniem do prac renowacyjnych. Przeprowadzono stosowną analizę numeryczną budynku uwzględniając w obliczeniach osłabione korozją elementy pomniejszając odpowiednio w modelu ich parametry wytrzymałościowe. Na tej podstawie zaproponowano stosowne wzmocnienia. Zastosowano technologie wzmocnień stosując wymianę profili stalowych, wspawywanie dodatkowych profili lub blach

  17. Clinical outcomes of Solis implantation for type Ⅱ and Ⅱ a Hangman's fractures%应用Solis融合器置入治疗Ⅱ型及Ⅱa型Hangman骨折的临床疗效

    Institute of Scientific and Technical Information of China (English)

    黄阳亮; 刘少喻; 梁春祥; 李浩淼; 于滨生; 龙厚清; 韩国伟; 张旭华; 魏富鑫

    2012-01-01

    目的:评价Solis椎间融合器置入治疗Ⅱ型及Ⅱa型Hangman骨折的临床疗效.方法:2006年1月~2011年11月,我科采用经前路Solis椎间融合器置入治疗Ⅱ型及Ⅱa型Hangman骨折患者17例,术中应用颈椎牵开器牵开椎间,通过紧张韧带进一步复位骨折.其中男11例、女6例,年龄19~41岁,平均26.2岁,按Levine-Edwards分类法Ⅱ型11例,Ⅱa型6例.于术后1周、3个月、6个月及末次随访时复查X线片,记录术前及末次随访时的枢椎移位及成角数据,记录术前及末次随访时的创伤后颈椎临床评分、颈部疼痛的VAS评分观察临床疗效.术前与末次随访时各数据间行t检验.结果:手术时间平均为78min (55~135min);术中出血量平均为22.5ml (10~50ml).术中无喉上神经、咽喉壁及血管损伤,术后无喉头水肿、血肿形成及伤口感染等手术并发症.平均随访25.1个月(6~48个月),手术节段及骨折部位平均于3个月时骨性融合,未见畸形愈合,末次随访时融合器无下沉、无移位、椎间无塌陷.创伤后颈椎临床评分:术前平均为48分(45~63分),末次随访时为92分(83~97分);颈部疼痛VAS评分术前平均为6.9分(6~9分),末次随访时为1.1分(0~2分);枢椎成角术前平均为-8.6°(-20~6°),末次随访时为+3.5°(0~+8°);枢椎移位术前平均为3.4mm( 1~5.5mm),末次随访时为1.2mm(0~2.0mm).以上两组数据间差异均有统计学意义(P<0.05).结论:应用Solis椎间融合器置入治疗Hangman骨折可行,手术操作简单、耗时短、创伤小、融合率高且有利于颈椎稳定.%Objectives: To investigate the clinical outcomes of Solis implantation for type II and II a Hangman's fractures. Methods: From January 2006 to November 2011, a total of 17 cases with type II and II a Hangman's fractures underwent anterior Solis placement. There were 11 males and 6 females, with the average age of 26.2 (range, 19-41). The reduction was achieved by

  18. Mikrofinancie ako nástroj rozvojovej pomoci

    OpenAIRE

    Klačková, Zuzana

    2010-01-01

    In the 20th century, microfinance went through a revolution: the number of microfinance was expanding exponentially all over the world. Microfinances are supposed to be one of the most efficient tools in alleviating poverty. The paper, Microfinance: Development Aid tool is analyzing whether microfinance institutions provide, beside the credit services, also preventive antimalarial services so as to lower malaria cases. Theoretical part is focused on definition and familiarization with 'microf...

  19. Podniková identita ako konkurenčná výhoda

    OpenAIRE

    Misun, Juraj

    2009-01-01

    Corporate identity, which has been in the middle of interest of practice for almost 40 years, is still a very important instrument in the field of Marketing and Management despite the currently unfavourable economic situation. Although consumers are checking prices the further the more, brand image is still an important factor in their decision making. What is more, every economist knows that periods of recession are usually followed by a period of expansion. Right now is a period when compan...

  20. Zákaznícky servis ako nástroj CRM

    OpenAIRE

    Smiková, Lenka

    2009-01-01

    The bachelor thesis analyzes and assesses the marketing mix of Prague City Apartments, s.r.o. It is focused on functioning of customer service as a part of the concept of Customer Relationship Management (CRM). The analysis is based on theoretical knowledge of marketing of services and CRM and on the importance of customer service in rendition of services, particularly in tourism and hotel industry. The thesis provides, via SWOT analysis, suggestions for improving quality of customer service ...

  1. Guerilla marketing ako nový trend v marketingovej komunikácii

    OpenAIRE

    Baraniaková, Júlia

    2010-01-01

    The goal of the bachelor thesis is to theoretically define guerrilla marketing as one of the new trends in marketing communication and to point out its advantages and disadvantages compared to traditional marketing communication. Moreover, the aim is to show its practical use on a company active on the Czech market (Nike CR) by individually interviewing its current marketing manager for CR and SR who explains the company's strategy in the field of guerilla marketing. Finally, the goal is to e...

  2. Bitcoin ako nová hrozba pre finančnú stabilitu?

    OpenAIRE

    Jana Drutarovská

    2013-01-01

    Cieľom predkladaného článku je definovať základné podmienky finančnej stability a analyzovať možné hrozby novej experimentálnej virtuálnej meny bitcoin na finančnú stabilitu. Úlohou príspevku je definovať oblasti a faktory, v ktorých táto nová virtuálna mena môže potenciálne spôsobovať finančnú nestabilitu.

  3. Firemná filantropia ako súčasť konceptu Corporate Social Responsability

    OpenAIRE

    Čarská, Viktória

    2010-01-01

    The thesis deals with the concept of corporate philanthropy and Corporate Social Responsibility. Our goal is to review the state of corporate philanthropy and CSR in SHP Harmanec, JSC, to explore, by a questionnaire research, the attitudes of young people to 30 years and to draw recommendations for the streamlining of corporate philanthropy in the company. Using the synthesis of the research`s results, it aims to design communication activities, which could present the philanthropic activitie...

  4. LOH 2016: Kto odíte ako víťaz?

    OpenAIRE

    Hatyinová, Petra

    2014-01-01

    This paper examines determinants of national medal success at the Summer Olympic Games between 1996 and 2012. Does hosting the Games have a positive impact on the number of gained medals? Do neighboring countries benefit from the approximate home advantage? Analysis based on the Tobit model confirmed the positive effect of hosting the event in the case of current as well as future host. The effect of the neighboring country was not proved. In-sample predictions test quality of the underlying ...

  5. KOGNITÍVNE SKRESLENIA AKO SÚČASŤ SPRISAHANECKÝCH TEÓRIÍ

    Directory of Open Access Journals (Sweden)

    Imrich Ruisel

    2015-09-01

    Full Text Available The beginnings of modern Greek philosophy have already indicated that man is not a reliable information processor, because he is unable to completely encompass the real world at a cognitive level. Thus, already Plato distinguished between the knowledge of the real true belief (epistémé and opinion (doxa. F. Bacon assumed that human senses are not the measure of reality. Psychologists A. Tversky and D. Kahneman pointed out that people do not follow rational arguments and set rules in their judgments. Rather, they use heuristics, which are simplified judgment rules that enable quick judgments, often biased by individual’s subjective beliefs. These wrong conclusions induce false ideas or fallacies. There are deductive and inductive fallacies. We contemplate the ratio of cognitive fallacies in forming and existence of conspiracy theories. Deliberate use of fallacies and biases is characteristic of the use of conspiracy theories in propaganda. Wrong judgments are made due to wrong understanding of coincidental events, insufficient processing of the laws of statistical regression and the tendency to search for confirmation. Preferred are verbal techniques in the form of naive and ritualized terms and statements based on unidentifiable fallacies.

  6. Vytvorenie internetového obchodu, ako nadstavba kamenného obchodu spoločnosti

    OpenAIRE

    Mokrý, Richard

    2012-01-01

    This diploma thesis concerns with electronic business. The technology of the trade, the internet and it's services and the history is described in the first chapter. Next chapter concerns with the general theoretical issues of electronic business. Defines relationships of particular subjects, such as client, seller or government service that appear in the terms and conditions of electronic business. Then describes the general model of electronic business. The third chapter is dedicated to gen...

  7. Franchising ako jedna z možných foriem vstupu podniku na medzinárodné trhy

    OpenAIRE

    Telgárska, Lýdia

    2006-01-01

    Práca pojednáva o franchisingu zo všeobecného hľadiska, to znamená teoretické vymedzenie, hlavné rysy franchisingu a výhody a nevýhody tejto formy podnikania. Ďalej sa zaoberá franchisingovými zmluvami, postupom ich uzatvárania a vzťahmi, ktoré vznikajú medzi poskytovateľom a príjemcom franšízy. Taktiež sa v nej rozoberajú špecifické rysy medzinárodného franchisingu a v neposlednej rade aplikácia franchisingu v medzinárodnej spoločnosti Starbucks.

  8. Vodcovstvo ako úspešný predpoklad pre dosahovanie zvyšovania konkurenčnej schopnosti podnikov

    OpenAIRE

    Hudakova, Ivana

    2008-01-01

    The idea of leadership has fascinated people for centuries. Leadership, or leading, is an asocial process, where the leader is seeking for voluntary participation of the subordinated, thus striving to achieve the company's objectives. Leadership clearly proves to be more than exercising power and authority. It also covers a number of managerial abilities, such as susceptibility, motivation, scrupulousness, empathy and communication. Leadership has to be present at all levels of the organizati...

  9. Talent manažment ako súčasť konkurenčnej výhody

    OpenAIRE

    Misun, Juraj; Hudakova, Ivana

    2011-01-01

    The current period is one full of unforeseen changes. A major problem for most Slovak businesses, as well as foreign companies finding themselves in a difficult and turbulent business environment, is the difficulty to find good quality employees. Even after they found them, it is difficult to keep them, especially if they achieve excellent results at work. It is about managing talent, or talent management, becoming common and extremely popular on both domestic and foreign markets. The success...

  10. Informačné technologie ako nástroj na kontrolu a riadenie firemných procesov

    OpenAIRE

    Mihályiová, Viktória

    2014-01-01

    Mihályiová, V. Information technology as a tool to manage and control business processes. Bachelor thesis. Brno, 2014. The thesis is focused on the issue of information systems utilization in business practice to manage and support business processes. The content of this work are primarily a theoretical background definition and conducting an enterprise internal analysis for a subsequent fulfillment of the thesis's main goal, which is a proposal for future development direction of a selected ...

  11. Spoločenská zodpovednosť ako súčasť stratégie firmy

    OpenAIRE

    Pavelčíková, Katarína

    2010-01-01

    The main topic of thesis is the issue of corporate social responsibility, which gradually acquires new fans and so is gaining importance. The first part aims to describe the basic characteristics of the concept and then clarifying the procedure for the incorporation of social responsibility to the main business strategy. Latest part of the theoretical part points to need to distinguish from the concept of corporate philanthropy and Public Relations. The practical part of the thesis describes ...

  12. Rozhľady. Konštituovanie Hindčiny Ako Úradného Jazyka Indie

    OpenAIRE

    Nagy Peter

    2015-01-01

    The article deals with the Hindi language and its use as a language for official purposes from the late Middle Ages until its implementation in the Constitution of India in the year 1949. It reveals various ideas for promoting Hindi as a national (official) language of India. The main focus is on the thoughts of Mahatma Gandhi on Hindi as the lingua franca of India and its role in the replacement of English. Further it describes the process and struggle of its implementation as the official l...

  13. Úverové registre ako nástroj znižovania úverového rizika

    OpenAIRE

    Bohumel, Michal

    2013-01-01

    This thesis deals with the credit bureaus, followed by an analysis of credit bureaus in Slovak and Czech banking sector and an overview of the operation of credit bureaus of European countries. In the initial part of the thesis I explains key concepts such as credit bureaus, bank risk, credit risk, market risk, liquidity risk, operational risk. Then I deal with the issue of credit bureaus as an instrument of reducing credit risk. I discuss the importance of credit bureaus for banks and their ...

  14. Value-at-Risk ako základ moderného riadenia rizík

    OpenAIRE

    Grešlík, Tomáš

    2011-01-01

    The thesis discusses a relatively modern method of measuring market risk - Value at Risk, which is widely used in the area of risk management. The thesis in the first section deals with risk, particularly financial risk and its dividing. The aim of the second section is to familiarize the reader with the concept of Value at Risk, why and where Value at Risk is used, what are the advantages and disadvantages. This section also includes methods of calculating Value at Risk. Third section contai...

  15. Rozhľady. Konštituovanie Hindčiny Ako Úradného Jazyka Indie

    Directory of Open Access Journals (Sweden)

    Nagy Peter

    2015-03-01

    Full Text Available The article deals with the Hindi language and its use as a language for official purposes from the late Middle Ages until its implementation in the Constitution of India in the year 1949. It reveals various ideas for promoting Hindi as a national (official language of India. The main focus is on the thoughts of Mahatma Gandhi on Hindi as the lingua franca of India and its role in the replacement of English. Further it describes the process and struggle of its implementation as the official language in the Constituent Assembly of India and concludes with the compromise made by this Assembly.

  16. Moderné matky ako cieľový segment pre produkty vybranej spoločnosti

    OpenAIRE

    Greifová, Daniela

    2012-01-01

    The purpose of the thesis is to describe in detail the segment of the modern moms as the target group for the products of the chosen company, in this case of Procter&Gamble, to demonstrate the concrete way how to use the theory about this segment and about current marketing by means of its successful campaign that targeted this segment and to show the dissimilarities between Czech and Slovak mothers that are part of this segment and the characteristics of this segment resulting from the globa...

  17. Biotransformation of Ferulic acid to 4-Vinylguaiacol by Enterobacter soli and E. aerogenes

    Science.gov (United States)

    We investigated the conversion of ferulic acid to 4-vinylguaiacol (4-VG), vanillin, vanillyl alcohol and vanillic acid by five Enterobacter strains. These high-value chemicals are usually synthesized using chemical methods but biological synthesis adds value. Ferulic acid, a relatively inexpensive...

  18. Development of foundry cores based on inorganic salts: Razvoj livarskih jeder na podlagi anorganskih soli:

    OpenAIRE

    Adámková, Eliška; Beňo, Jaroslav; Jelínek, Petr; Mikšovský, František

    2013-01-01

    The aim of this study is to describe the possibilities of using salt cores for gravity, low-pressure or high-pressure die-casting technology. Determinations of the primary, secondary and final residual strengths were carried out in order to evaluate the possibilities of the salt-core utilization. Furthermore, this contribution is focused on developing composite salts with better mechanical properties. The solubility of these cores and a possibility of their reclamation in a closed cycle with ...

  19. The Prokaryotic Expression and Bioactivity of the Recombinant Red Fire Ant Venom Allergen Soli4

    Institute of Scientific and Technical Information of China (English)

    HAN Xue-qing; LIN Xiang-mei; CHEN Hong-jun; ZHANG Yong-guo; YE Gui-sheng; WU Shao-qiang; LI Jian; CHEN Nai-zhong; CHEN Yan; ZHU Shui-fang

    2009-01-01

    The sting of red imported fire ant (RIFA) could cause serious allergic response in fraction of people. These allergic reactions are mainly caused by its venom, especially venom allergen Sol I 1-4. To produce large amount of RIFA venom allergen Sol I 4 for diagnosis of RIFA allergy and allergen-specific immunotherapy, the gene encoding this protein was amplified and cloned into the prokaryotic expression vector pET43, la. The recombinant plasmid was used to transform competent cells and the recombinant proteins were expressed in E. Coli. SDS-PAGE and Western blotting analysis indicated that high-level expression of Sol I 4 protein was successfully achieved. Allergenic activity analysis of the recombinant allergen Sol I 4 was then performed on rabbit. The result showed that the recombinant protein obtained had significant allergenic activity. It indicated that the recombinant allergen Sol I 4 of RIFA venom was successfully expressed in E. Coli, which provided foundation for further developing therapeutic and diagnosis reagents of RIFA allergy.

  20. Analýza nástrojov internetu ako média pre marketingové účely

    OpenAIRE

    Gregová, Silvia

    2010-01-01

    The goal of this thesis is the analysis of internet marketing tools and implementation of acquired knowledge into practice. This topic is particularly important to study because the current marketing and communication mix in the world and the Czech Republic are increasingly concerned in on-line communications. Leading topics of this work are: Marketing and Commercial Communications, Internet as a Modern Medium, Internet and its Using in Commercial Communications, Internet Portal www.hotel-uby...

  1. Peniaze ako zdroj šťastia v našich životoch? (Is Money a Source of Happiness in our lives?

    Directory of Open Access Journals (Sweden)

    Otakar Horák

    2013-06-01

    Full Text Available This paper begins by referring to the research which distinguishes two different aspects of subjective well-being, namely 1. experiences of happiness – measured by frequency and intensity of joy, stress, anger, worry and sadness we experience at a certain moment and 2. life satisfaction. Survey shows that beyond an annual household income of $75 000 there is no increase in the experiences of happiness. The paper then refers to the psychological mechanisms and factors – namely hedonic adaptation, social comparison and stress as a result of a raise – that lower the capacity of money to buy us the experiences of happiness. Not only we get quickly used to the things we buy, money also puts us into stressful situations and makes us build barriers between people. The paper then specifies strategies of economic decision-making that contribute to the maximization of happiness, such as purchase of the experiences instead of the things, interrupted and postponed consumption, or prosocial spending. The research indicates that prosocial spending has higher causal impact on promoting happiness than personal spending. As a matter of fact, this phenomenon is not culturally limited to the West. Concluding assertion – expressed only in the form of hypothesis – says that prosocial spending which is a form of prosocial behavior strengthens – through production of happiness – mutual relations in the society (cooperation.

  2. Peniaze ako zdroj šťastia v našich životoch? (Is Money a Source of Happiness in our lives?)

    OpenAIRE

    Otakar Horák

    2013-01-01

    This paper begins by referring to the research which distinguishes two different aspects of subjective well-being, namely 1. experiences of happiness – measured by frequency and intensity of joy, stress, anger, worry and sadness we experience at a certain moment and 2. life satisfaction. Survey shows that beyond an annual household income of $75 000 there is no increase in the experiences of happiness. The paper then refers to the psychological mechanisms and factors – namely hedonic adaptati...

  3. Priateľstvo ako cnosť súkromná i verejná (Friendship as a Private and Public Virtues

    Directory of Open Access Journals (Sweden)

    Matúš Porubjak

    2008-03-01

    Full Text Available The aim of this article is to show a social role of friendship (filia in Classical Greece and to discover why, as Aristotle thinks, is filia the greatest good for the polis. The author bases his analysis on a comparison between Plato’s dialogue Lysis and Aristotle’s Politics (namely the book 1 and book 3. He distinguishes four types of filia, and shows the social role played by each one of them: the love of parents to children, the friendship between teenage boys of the same age, the love of elder men to boys, and the friendship between free adult citizens.

  4. Ekonomika inovácie ako súčasť zvyšovania výkonnosti firmy

    Directory of Open Access Journals (Sweden)

    Chromjaková Felicita

    2009-12-01

    Full Text Available Základom dobre nastavenej inovácie je jej správna ekonomika, teda znalosť prietoku hodnoty, participujúcej v rôznych etapách na tvorbe inovácií a komplexnom inovačnom procese firmy. Prietok hodnoty má zásadný význam pre ekonomiku inovácie v tom, že na základe optimálne definovaných inovačných metrík dokáže identifikovať reálne ekonomické vstupy a výstupy a priamo poukázať na inovačný potenciál firmy a jeho úspešnosť z hľadiska zvyšovania budúcej výkonnosti firmy.The basis of a well balanced innovation is its right economy, that is so the knowledge of the value throughput, participating in the various phases of creating the innovation and of a complex innovation process of the company. Value throughput has an essential importance for the innovation economy there, where on the basis of optimally defined innovation metrics can be identified real economical inputs and outputs and directly the innovation potential of the ompany and its success from the point of view of the increase of its future efficiency.

  5. Problémy v komunikácii ako príčina vzniku neúplných rodín

    OpenAIRE

    MORAVČIKOVÁ, Petra

    2013-01-01

    Submitted thesis tries to show importance and irreplaceable space in communicatin at present. It also deals with the need for communication in the basic cell of society - the family. It speaks about the importance and need for mutual communication and the correct listening in families. It deals with the causes of matrimonial crisis and consequences of cinflicts in the family. It speaks about communication differences between man and woman, communication barrires, which causes problems in fami...

  6. Methanisation of stackable biomass in discontinuously operated solis fermentation plants; Methanisierung stapelbarer Biomassen in diskontinuierlich betriebenen Feststofffermentationsanlagen

    Energy Technology Data Exchange (ETDEWEB)

    Kusch, S.

    2007-07-01

    In contrary to the municipal waste processing in agricultural areas the biogas production based on solid materials is still in a phase of development. A possible processing approach is the discontinuously operated box fermenter with irrigation. Based on associated features like robustness, flexibility and utilizability in agricultural farms without liquid manure this type of fermenter is quite attractive.The author describes the laboratory and field tests and discusses possible applications, including procedural boundary conditions using different materials and problematic aspects. Possibilities for process optimizations are given.

  7. Pamäť ako múza: alétheia medzi enthusiasmom a epistémé (Memory as Muse: Alētheia between enthousiasm and epistēmē

    Directory of Open Access Journals (Sweden)

    Zuzana Zelinová

    2015-09-01

    Full Text Available The contribution underlines the role of Mnēmosynē (Memory as the mother of the Muses as a constitutive aspect of archaic poetry based on the etymological relationship between alētheia (as true knowledge and lēthē (as forgetting, in which truth becomes – in a specific way – the negation of forgetting. The social role of the poet, who participates in perpetuation of truth, is closely related to the complementarity produced by Muse and Memory. The main purpose of this paper is to offer an answer to the question: “How can be this relationship transformed from the area of poiētikē technē to the area of philosophy and what is the place of Mnēmosynē in Plato´s anamnēsis (dialogue Meno?”

  8. Porovnanie aktivít CSR nadnárodnej spoločnosti ako celku a jej aktivít v rámci ČR

    OpenAIRE

    Konkoľová, Karolína

    2012-01-01

    The bachelor thesis deals with the topic of Corporate Social Responsibility (CSR). The aim is to compare CSR activities carried out by an international company on a supranational level with the activities realized on a local level in Czech Republic. The bachelor thesis is divided into two parts, theoretical and practical one. The theoretical part deals with the concept of CSR, standards and organisations acting in this field and CSR reporting. The practical part goes into CSR of a global comp...

  9. Podnikové výdavky na výskum a vývoj ako súčasť inovačnej kapacity v podmienkach konkurujúcich si odvetví

    OpenAIRE

    Hudec, Oto; Prochádzková, Martina

    2013-01-01

    Purpose of the article: This paper examines how firms ‘internal factors and their industrial affiliation influence the amount of business expenditures on research and development. Methodology/methods: The means for expressing that relation is the hierarchical linear model, in which the fixed explanatory variables are quantitative business indicators that include the number of employees, operational profit and capital expenditures. The qualitative explanatory factor is the industry...

  10. Segmentácia trhu pri použití marketingových dát ako súčasť marketingovej stratégie

    OpenAIRE

    Nagyová-Tkáčová, Monika

    2013-01-01

    The thesis deals mainly with market segmentation which is very important part of marketing strategy. The theoretical part consists of general knowledge of marketing, a Customer relationship management (CRM), market segmentation and database marketing. A case study consists of the implementation of the theoretical knowledge into the practical part. The aims of the thesis are: an introduction to the issue of market segmentation, an importance of market segmentation in creating marketing strateg...

  11. Teoretické vymedzenie podnikového emailingu ako nástroja podnikového správania v systéme podnikovej identity

    OpenAIRE

    Misun, Juraj

    2010-01-01

    Corporate Behavior is according to many renowned authors the most important instrument of the corporate identity. It defines how all employees of a company behave to themselves and to the external environment. At present time the email communication became very important not only for private use, but also to companies. Therefore companies should use an instrument of the corporate behavior called the corporate emailing.

  12. Vernostné programy v leteckej spoločnosti ako nástroj riadenia vzťahov so zákazníkmi

    OpenAIRE

    Demčáková, Vanda

    2015-01-01

    This bachelors thesis Loyalty programs as a CRM tool used by airline describes the importance of Customer Relationship Management in todays competitive market and explains basic concepts of a relationship between organization and customers, such as customer value, satisfaction and loyalty. In the practical part is the knowledge from theoretical part applied on the specific airline. The practical part presents an assessment and evaluation of the loyalty program OK Plus Partner. To carry out th...

  13. Operant learning and differential-reinforcement-of-low-rate 36-s responding in 5-HT1A and 5-HT1B receptor knockout mice.

    NARCIS (Netherlands)

    Pattij, T.; Broersen, L.M.; Linde, J. van der; Groenink, L.; Gugten, J. van der; Maes, R.A.A.; Olivier, B.

    2003-01-01

    Previous studies with mice lacking 5-HT(1A) (1AKO) and 5-HT(1B) (1BKO) receptors in hippocampus-dependent learning and memory paradigms, suggest that these receptors play an important role in learning and memory, although their precise role is unclear. In the present study, 1AKO and 1BKO mice were s

  14. 旱作土壤耕层及其肥力培育途径%The Soli Cultiv aet d Layer in Drylan d and Technical Patterns in Cul tivating Soil Fertilti y

    Institute of Scientific and Technical Information of China (English)

    韩晓增; 邹文秀; 陆欣春; 段景海

    2015-01-01

    耕作层是人类为了栽培作物,利用工具对土壤进行扰动的深度层。耕作层的结构和厚度决定了作物的生存环境及养分和水分的供给。随着对粮食需求的不断增加,培育一个肥沃而深厚的耕作层,提高土壤地力得到了越来越多人的关注。文章综合分析了耕作层的演变和功能及其对作物根系生长和产量的重要意义,由此提出了粘质土壤(粘粒含量>35%)的适宜耕作层深度为0~35 cm,沙质土壤为0~20 cm;综述了秸秆和有机肥还田在培育土壤耕作层方面的研究进展。随着种植结构的多元化和农用机械的不断更新,对未来土壤耕作层培育的技术模式进行了展望,即建立集成作物轮作、有机物料还田、耕作强度和频度的综合技术模式,旨在为合理耕作层培育提供理论支撑。图1,参49。%The soil cultivated layer is that human activity disturbed the depth of soil by tools in order to cultivate crops .The structure and depth of soil cultivated layer determined the habitat and supply of nutrient and water for crop .The construction of fertile and deep cultivated soil layer and improvement of soil fertility were attracting more and more attention with the increased demand for food .The e-volution and function of soil cultivated layer and its effect on crop root and yield were reviewed comprehensively , suitable depths of cul-tivated layer were suggested for clay soil ( clay content >35%) with 0~35 cm depth and sandy soil with 0~20 cm depth based on a-bove mentioned.The influence of straw and organic matter incorporation on soil cultivated layer was discussed.The prospective of tech-nical patterns of soil cultivated layer , including the integrated technical pattern involving crop rotation , straw and organic matter incor-poration , depth and frequency of soil tillage , which will provide relevant reference for the construction of suitable soil cultivated layer .

  15. Applci ation of TOPSIS Based on Entropy Weight in Soli Qualti y Assessment of Songhua River Area%基于熵权的TOPSIS模型在松花江地区土壤评价应用

    Institute of Scientific and Technical Information of China (English)

    张春宇

    2015-01-01

    Soil is the material of agriculture base , therefore , preservation and improvement of soil quality is the basic task for agricultural sustainable development.Based on Songhua river area as an example , this paper refered other documents and combined the actual situation of the study area to grade , attempt to using TOPSIS model from practicality and versatility of soil quality are analyzed.From the results can be clearly seen the region′s soil types of good or bad sort , for future scholars can provide powerful evidence.%以松花江地区为例,参考已有文献,选取指标和等级划分标准,利用TOPSIS模型从实用性、多功能性对土壤质量进行分析。从得的到的结果中可以很清晰看出各种土质量由好到坏的排序,并为今后土壤的综合评价提供有力依据。

  16. Apricot Kernel Oil Ameliorates Cyclophosphamide-Associated Immunosuppression in Rats.

    Science.gov (United States)

    Tian, Honglei; Yan, Haiyan; Tan, Siwei; Zhan, Ping; Mao, Xiaoying; Wang, Peng; Wang, Zhouping

    2016-08-01

    The effects of dietary apricot kernel oil (AKO), which contains high levels of oleic and linoleic acids and lower levels of α-tocopherol, were evaluated in a rat model of cyclophosphamide-induced immunosuppression. Rats had intraperitoneal injection with cyclophosphamide to induce immunosuppression and were then infused with AKO or normal saline (NS) for 4 weeks. Enzyme-linked immunosorbent assays were used to detect antimicrobial factors in lymphocytes and anti-inflammatory factors in hepatocytes. Hematoxylin & eosin staining was conducted prior to histopathological analysis of the spleen, liver, and thymus. Significant differences were observed between the immune functions of the healthy control group, the normal saline group, and the AKO group. Compared to the normal saline-treated group, lymphocytes isolated from rats administered AKO showed significant improvement in immunoglobulin (Ig)A, IgM, IgG, interleukin (IL)-2, IL-12, and tumor necrosis factor-α (TNF-α) levels (p cyclophosphamide-associated organ degeneration. These results suggested that AKO may enhance the immune system in vivo. These effects may reflect the activities of intermediate oleic and linoleic acid metabolites, which play a vital role in the immune system, and the α-tocopherol in AKO may further enhance this phenomenon. Thus, the use of AKO as a nutritional supplement can be proposed to ameliorate chemotherapy-associated immunosuppression. PMID:27262314

  17. From Commonwealth to Postcolonial Literature

    OpenAIRE

    Ako, Edward O.

    2004-01-01

    In his paper,"From Commonwealth to Postcolonial Literature," Edward O.Ako begins by establishing the link between Empire and English-language literature. Drawing on Elleke Boehmer, Gauri Viswanath, and Terry Eagleton, Ako discusses the ideological uses of literature and argues that the textual forms that emerged as "resistance" to imperial domination were referred to by a multiplicity of terms: Commonwealth Literature, New English Literature, Literature in English, Third World Literature, Wor...

  18. Rozprávka ako nástroj spoločenskej satiry vo francúzskej literatúre 18. storočia: Charles Duclos a jeho Acajou a Zirphile (1744 (Le conte comme l’outil de la satire sociale dans la littérature française du XVIIIe siècle : Charles Duclos et son Acajou et Zirphile (1744

    Directory of Open Access Journals (Sweden)

    Andrea Tureková

    2012-12-01

    Full Text Available Le présent article est consacré à la problématique du conte libertin du XVIIIe siècle et plus particulièrement à sa dimension satirique à l’égard de la société contemporaine. Sur l’exemple du conte de Charles Duclos, Acajou et Zirphile (1744, l’auteur analyse les différentes facettes que prend la satire sociale dans ce conte, qui se veut une « sottise ».

  19. Long- and Short-Term Processes Indicated by the Displacement of the Chamber Roofs in the Monumental Wieliczka Salt Mine / Długo- i krótkotrwałe procesy sygnalizowane przemieszczeniami stropu komór w zabytkowej kopalni soli wieliczka

    Science.gov (United States)

    Kortas, Grzegorz

    2013-03-01

    This paper presents several processes that are characteristic for the salt rock mass, which have been found upon analysis of observations of the chamber roof displacements in the monumental Wieliczka Salt Mine. Those processes are revealed in different time frames and they range from short- to long-term ones. A regular long-term increase or decrease of creeping rate in the chamber roof is expressed by power function of time. It was found that an annual component of that process consists in the season cycle, marked by increased displacement rates in the summer and reduced ones in the winter. The seasonal changes in air humidity are the cause of those cycles. A rock slip cycle is observed in some chambers during several months. In those periods, we can identify displacements that happen when internal static and dynamic friction is overcome. In 2007-2009, single signals from large-area rock-mass movements were recorded in several chambers. They were probably associated with the change of geo-mechanical conditions in northern region of rock mass after a leak had been plugged in the cross-corridor Mina. The results of our analysis have revealed new cognitive components contributing to the description of rock behaviour in specific geological and mining conditions of Miocene salt deposits.

  20. Estonian science breeds world-class researches / ToivoTänavsuu, Hille Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Toivo

    2010-01-01

    Eesti teadusfirmadest: biotehnoloogia ettevõtted Celecure AS, Protobios OÜ, Icosagen AS, Asper Biotech AS ja Solis BioDyne OÜ, geenitehnoloogia firma Genorama OÜ, keemiatehnoloogia ettevõte AS Cambrex Tallinn

  1. 3Corners of the World / Joosep Sang

    Index Scriptorium Estoniae

    Sang, Joosep

    2009-01-01

    Arvustused : Robert Jürjendal, Petri Kuljuntausta, David Rothenberg. 3Corners of the World. [Tallinn] : Terra Nova Music, 2008. Nadia ja Fragile. Uhke ali. [Tallinn] : Nadia & Fragile, 2008. UMA. Civitas Soli. [Tallinn] : Elwood, 2008. Uutest heliplaatidest

  2. Coverage Bias and Sensitivity of Variant Calling for Four Whole-genome Sequencing Technologies

    OpenAIRE

    Rieber, Nora; Zapatka, Marc; Lasitschka, Bärbel; Jones, David,; Northcott, Paul; Hutter, Barbara; Jäger, Natalie; Kool, Marcel; Taylor, Michael; Lichter, Peter; Pfister, Stefan; Wolf, Stephan; Brors, Benedikt; Eils, Roland

    2013-01-01

    The emergence of high-throughput, next-generation sequencing technologies has dramatically altered the way we assess genomes in population genetics and in cancer genomics. Currently, there are four commonly used whole-genome sequencing platforms on the market: Illumina’s HiSeq2000, Life Technologies’ SOLiD 4 and its completely redesigned 5500xl SOLiD, and Complete Genomics’ technology. A number of earlier studies have compared a subset of those sequencing platforms or compared those platforms...

  3. Coverage Bias and Sensitivity of Variant Calling for Four Whole-genome Sequencing Technologies

    OpenAIRE

    Nora Rieber; Marc Zapatka; Bärbel Lasitschka; David Jones1; Paul Northcott; Barbara Hutter; Natalie Jäger; Marcel Kool; Michael Taylor; Peter Lichter; Stefan Pfister; Stephan Wolf; Benedikt Brors; Roland Eils

    2013-01-01

    The emergence of high-throughput, next-generation sequencing technologies has dramatically altered the way we assess genomes in population genetics and in cancer genomics. Currently, there are four commonly used whole-genome sequencing platforms on the market: Illumina's HiSeq2000, Life Technologies' SOLiD 4 and its completely redesigned 5500xl SOLiD, and Complete Genomics' technology. A number of earlier studies have compared a subset of those sequencing platforms or compared those platforms...

  4. The Promotion of Teaching Excellence in Higher Education: A Comparison of the Australian and New Zealand Approaches

    Science.gov (United States)

    Abbott, Malcolm

    2012-01-01

    In this paper a comparison is made between the structure and operations of the Australian Learning and Teaching Council and the Ako Aotearoa National Centre for Tertiary Teaching Excellence of New Zealand. Both of these organisations were established in the mid 2000s and were created at a time when higher education institutions in both countries…

  5. Promoting Competence and Resilience in the School Context

    Science.gov (United States)

    Masten, Ann S.; Herbers, Janette E.; Cutuli, J. J.; Lafavor, Theresa L.

    2008-01-01

    Four decades of research on resilience in young people provide compelling data and models for applications in the school context. Resilience theory and findings are highly congruent with Strengths-Based School Counseling (SBSC) as formulated by Galassi and Akos (2007). In this article, resilience is defined in relation to competence in…

  6. School Counselors' Attitudes towards Providing Services to Students Receiving Section 504 Classroom Accommodations: Implications for School Counselor Educators

    Science.gov (United States)

    Romano, Dawn M.; Paradise, Louis V.; Green, Eric J.

    2009-01-01

    Questions have arisen regarding counselor's capabilities in assisting students with special needs (Milsom & Akos, 2003; Studer & Quigney, 2005). This study examined school counselors' training and attitudes toward providing services to students with learning disabilities who qualified for services only under Section 504 of the Rehabilitation Act.…

  7. Ühepäevaliblika lend üle valgete laikude / Sven Karja

    Index Scriptorium Estoniae

    Karja, Sven, 1968-

    2000-01-01

    Teatrikriitika olukorrast - kiirtempo, esietenduse põhjal arvustamine, väikelinnateatrite keskmised lavastused jäävad kajastuseta. Vaadeldakse hooaja kolme "mahavaikitud " lavastust - Akos Kertészi "Lesed" Eili Neuhausi lavastuses Rakvere Teatris, Mario Fratti "Ohver" Madis Kalmeti lavastuses Pärnu Endlas ja David Williamsoni "Sõbrad ja raha" Viljandi Ugalas.

  8. Lack of Adipocyte AMPK Exacerbates Insulin Resistance and Hepatic Steatosis through Brown and Beige Adipose Tissue Function.

    Science.gov (United States)

    Mottillo, Emilio P; Desjardins, Eric M; Crane, Justin D; Smith, Brennan K; Green, Alex E; Ducommun, Serge; Henriksen, Tora I; Rebalka, Irena A; Razi, Aida; Sakamoto, Kei; Scheele, Camilla; Kemp, Bruce E; Hawke, Thomas J; Ortega, Joaquin; Granneman, James G; Steinberg, Gregory R

    2016-07-12

    Brown (BAT) and white (WAT) adipose tissues play distinct roles in maintaining whole-body energy homeostasis, and their dysfunction can contribute to non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. The AMP-activated protein kinase (AMPK) is a cellular energy sensor, but its role in regulating BAT and WAT metabolism is unclear. We generated an inducible model for deletion of the two AMPK β subunits in adipocytes (iβ1β2AKO) and found that iβ1β2AKO mice were cold intolerant and resistant to β-adrenergic activation of BAT and beiging of WAT. BAT from iβ1β2AKO mice had impairments in mitochondrial structure, function, and markers of mitophagy. In response to a high-fat diet, iβ1β2AKO mice more rapidly developed liver steatosis as well as glucose and insulin intolerance. Thus, AMPK in adipocytes is vital for maintaining mitochondrial integrity, responding to pharmacological agents and thermal stress, and protecting against nutrient-overload-induced NAFLD and insulin resistance. PMID:27411013

  9. Symposium: Aesthetic Education in Japan Today

    Science.gov (United States)

    Okazaki, Ako; Masuda, Kingo; Kaneda, Takuya; Hino, Yoko; Okamoto, Yasuaki; Fukumoto, Kinichi; Nagamori, Motoki; Yamada, Kazumi; Motomura, Kenta; Ishizaki, Kazhiro; Okada, Masashi; Kaneko, Yoshimasa; Naoe, Toshio; Fujie, Mitsuru; Iwano, Masako

    2003-01-01

    The purpose of this symposium is to provide readers with a general understanding of Japanese art and aesthetics education and its interaction with other cultures. The essays cover a variety of topics, including historical, cross-cultural, theoretical, and practical perspectives. Following an introduction by Ako Okazaki, the following papers are…

  10. Maternal intravenous treatment with either azithromycin or solithromycin clears Ureaplasma parvum from the amniotic fluid in an ovine model of intrauterine infection.

    Science.gov (United States)

    Miura, Yuichiro; Payne, Matthew S; Keelan, Jeffrey A; Noe, Andres; Carter, Sean; Watts, Rory; Spiller, Owen B; Jobe, Alan H; Kallapur, Suhas G; Saito, Masatoshi; Stock, Sarah J; Newnham, John P; Kemp, Matthew W

    2014-09-01

    Intrauterine infection with Ureaplasma spp. is strongly associated with preterm birth and adverse neonatal outcomes. We assessed whether combined intraamniotic (IA) and maternal intravenous (IV) treatment with one of two candidate antibiotics, azithromycin (AZ) or solithromycin (SOLI), would eradicate intrauterine Ureaplasma parvum infection in a sheep model of pregnancy. Sheep with singleton pregnancies received an IA injection of U. parvum serovar 3 at 85 days of gestational age (GA). At 120 days of GA, animals (n=5 to 8/group) received one of the following treatments: (i) maternal IV SOLI with a single IA injection of vehicle (IV SOLI only); (ii) maternal IV SOLI with a single IA injection of SOLI (IV+IA SOLI); (iii) maternal IV AZ and a single IA injection of vehicle (IV AZ only); (iv) maternal IV AZ and a single IA injection of AZ (IV+IA AZ); or (v) maternal IV and single IA injection of vehicle (control). Lambs were surgically delivered at 125 days of GA. Treatment efficacies were assessed by U. parvum culture, quantitative PCR, enzyme-linked immunosorbent assay, and histopathology. Amniotic fluid (AF) from all control animals contained culturable U. parvum. AF, lung, and chorioamnion from all AZ- or SOLI-treated animals (IV only or IV plus IA) were negative for culturable U. parvum. Relative to the results for the control, the levels of expression of interleukin 1β (IL-1β), IL-6, IL-8, and monocyte chemoattractant protein 2 (MCP-2) in fetal skin were significantly decreased in the IV SOLI-only group, the MCP-1 protein concentration in the amniotic fluid was significantly increased in the IV+IA SOLI group, and there was no significant difference in the histological inflammation scoring of lung or chorioamnion among the five groups. In the present study, treatment with either AZ or SOLI (IV only or IV+IA) effectively eradicated macrolide-sensitive U. parvum from the AF. There was no discernible difference in antibiotic therapy efficacy between IV-only and IV

  11. Zabezpečení bezdrátových sítí a možné útoky na tyto sítě

    OpenAIRE

    Vlček, Peter

    2010-01-01

    Prvým z hlavných cieľov tejto práce bolo naštudovať a preskúmať rôzne typy útokov na bezdrôtové siete. Táto práca je zameraná na najbežnejšie sa vyskytujúce druhy útokov, akými sú WEP/WPA/WPA2 prelomenie, útok mužom uprostred (MIM), slovníkový útok, MAC podvrhnutie a nakoniec Denial of Service útoky. Popis jednotlivých útokov je zároveň doplnený podrobnými návodmi, ako sa dajú tieto útoky zrealizovať na platforme Windows. Ďalej je popísané, ako sa dajú jednotlivé útoky odhaliť a identifikovať...

  12. Evaluation of content of phenolics in Salvia species cultivated in South Moravian Region / Hodnotenie obsahu fenolov vo vybraných druhoch rodu Salvia L. pestovaných v Juhomoravskom kraji

    OpenAIRE

    Muráriková A.; Kaffková K.; Raab S.; Neugebauerová J.

    2015-01-01

    V štúdii na celkový obsah fenolových látok a kyseliny rozmarínovej bolo hodnotených 37 vzoriek šalvie (Salvia L.) z metanolových extraktov stanovených spektrofotometricky. Množstvo celkových fenolov bolo analyzované s pomocou Folin Ciocalteuového skúmadla. Ako štandard bola použitá kyselina galová a celkové fenoly boli vyjadrené ako mg.100 g-1 kyseliny galovej v sušine. Boli zobrazené podstatné rozdiely hodnotených extraktov. Všetky skúmané druhy okrem Salvia jurisici (990,79 mg GAE.100 g-1) ...

  13. Návrh modelu Savoniova rotoru

    OpenAIRE

    Sedlačko, Jozef

    2016-01-01

    Veterná energetika patrí v poslednom desaťročí k rýchlo rozvíjajúcim odvetviam výroby energie z obnoviteľných zdrojov. Savoniov rotor ako malý veterný zdroj bol rovnako ako ostatné zdroje podrobený skúmaniu a inováciám. Táto práca sa zaoberá návrhom a realizáciou Savoniovho rotoru pre laboratórne účely. V prvej časti práce je predostretý jeho princíp a vlastnosti. Druhá časť pojednáva o matematickom návrhu rozmerov a geometrie. V poslednej časti sa venuje meraniu a vyhodnoteniu základných par...

  14. Soljenje hrane i kronične bolesti

    OpenAIRE

    Mimica, M.; KRAPAC, L.

    1980-01-01

    U epidemiološkom istraživanju kroničnih bolesti 1972. godine u uzorku stanovništva koji se sastojao od 3 265 ispitanika u dobi od 38 do 57 godina, dobivene su izjave o soljenju hrane. Najveći broj ispitanika (62,8%) izjavio je da »normalno« soli hranu, 17,0% »dosoljava«, a 1,6% »dosoljava mnogo« hranu. »Malo soli« hranu 16,1% a »ne soli« hranu 2,5% ispitanika. Muškarci u prosjeku više dosoljavaju hranu nego žene, a seosko stanovništvo više nego gradsko. Nisu nađene razlike u prevalenciji sist...

  15. Desni ekstremizam, radikalizam i zapadnoeuropska Nova desnica

    OpenAIRE

    Velički, Damir

    2010-01-01

    U ovom se radu polazi od pojma političkog ekstremizma koji karakterizira odbijanje temeljnih vrijednosti demokratske ustavne države te se, nadalje, nakon definiranja strukturnih značajki desnog ekstremizma on razgraničuje od srodnog pojma desnog radikalizma. U nastavku se analizira pojam tzv. Nove desnice te njezina prisutnost u Francuskoj, Italiji, Velikoj Britaniji i SR Njemačkoj. Pojam Nova desnica prikladan je ako se njime opisuju intelektualna strujanja koja rade na ide...

  16. Planning and realization of broadband network in accordance with guidelines from European digital agenda

    OpenAIRE

    Simšič, Jaka

    2016-01-01

    Thesis contains description of European Digital Agenda (EDA) guidelines regarding the need for fast and ultra fast internet access. Republic of Slovenia supported those guidelines in document Digital Slovenia 2020 and with that confirmed the intention to fulfill the requirements set by European Commission in EDA. Current state of communication network does not suffice those guidelines. According to Agency for communication networks and services of the Republic of Slovenia (AKOS) copper connec...

  17. Histoire et onomastique médiévales. L'enquête de 1249 sur la guerre de Thibaud I de Navarre en Labourd

    OpenAIRE

    Orpustan, Jean-Baptiste

    1997-01-01

    XIII-garren mendean Nabarrako errege Zantxo Azkarra hil eta (1234), haren iloba Thibalt edo Thibaud Xampañako kondeak ukan zituen Nabarrako korona eta erresuma. Berehala gataskak hasi ziren orduan Angeleterrako erregeren meneko ziren Baiones eta Lapurtarren eta Nabartarren artean : artalde eta abere bahitze larre-mendietan, untzietan zeramatzaten salgai ebaste eta zergatze Baionan eta portu angelesetan... Gauzak oraino gaixtatu ziren, bertze guzien artetik Garroko jauregia, hango jaunak Thiba...

  18. Incorporation monitoring in Germany. Quo vadis?; Inkorporationsueberwachung in Deutschland. Quo Vadis?

    Energy Technology Data Exchange (ETDEWEB)

    Froning, Martina; Breustedt, Bastian; Scholl, Clemens [Fachverband fuer Strahlenschutz e.V., Garching (Germany). Arbeitskreis Inkorporationsueberwachung

    2015-07-01

    In 1970 a study group incorporation monitoring (AKI) was founded in the research center Karlsruhe. In the last decade the AKO and the respective group in the Bundesamt fuer Strahlenschutz (BfS) have identified the necessity to develop a test point concept. The actual state of incorporation monitoring in Germany is, the future of the test point concept and related questions are discussed in the contribution.

  19. Phenolic compounds variation in Mentha L. Species in the course of a four-years period / Kolísanie fenolových látok v rôznych druhoch Mentha L. Počas 4 ročného obdobia

    Directory of Open Access Journals (Sweden)

    Fialová S.

    2015-06-01

    Full Text Available Mäty patria medzi významné rastliny čeľade Lamiaceae. Okrem silice obsahujú množstvo cenných fenolových látok, vrátane flavonoidov a fenolových kyselín, ktoré sa spolupodieľajú na farmakologickej aktivite mäty. V našej práci sme hodnotili kolísanie obsahu fenolových látok počas 4 rokov vegetácie. Pri 1- až 4-ročných rastlinách sme porovnali v suchých listoch obsah hydroxyškoricových derivátov vyjadrených ako kyselina rozmarínová (λ = 505 nm, polyfenolov a trieslovín vyjadrených ako kyselina rozmarínová (λ = 760 nm a flavonoidov vyjadrených ako luteolín-7-O-glukozid (λ = 392 nm a kvercetín (λ = 420 nm. Využili sme spektrofotometrické metódy Európskeho liekopisu. Naše výsledky poukazujú na vysoké hodnoty fenolových látok najmä v 3- a 4-ročných porastoch.

  20. New Initiatives for Synoptic Observations

    Indian Academy of Sciences (India)

    C. U. Keller

    2000-09-01

    Several new synoptic facilities for long-term studies of the Sun will become operational within the next few years. This paper summarizes information on some of these projects, in particular GONG+, ISOON, GOES/SXI, and SOLIS. SOLIS, the Synoptic Optical Long-Term Investigations of the Sun, is currently being built by the National Solar Observatory and will become operational in 2001. It consists of a 50-cm vector spectromagnetograph, a 14-cm full-disk patrol, and an 8-mm sunas-a-star spectrometer.

  1. DOLOČANJE KONCENTRACIJE NATRIJA IN KALIJA V URINU TER VPLIV NA KRVNI TLAK PRI ODRASLIH PREBIVALCIH ZAHODNE SLOVENIJE

    OpenAIRE

    Vukina, Ana

    2015-01-01

    Čezmeren vnos natrija oziroma soli je pomemben prehranski dejavnik tveganja za zdravje in eden od glavnih vzrokov za nastanek kronično nenalezljivih bolezni: povišanega krvnega tlaka, bolezni srca in ožilja, bolezni ledvic, sladkorne bolezni, čezmerne hranjenosti in debelosti ter nekaterih vrst raka. Z magistrskim delom smo želeli ugotoviti in prikazati obstoječe stanje uživanja natrija in kalija pri odraslih prebivalcih zahodne Slovenije ter predstaviti problematiko prekomernega vnosa soli k...

  2. Eestlased rajavad omaenda oaasi Ränioru mägismaal / Martin Hanson

    Index Scriptorium Estoniae

    Hanson, Martin, 1984-

    2008-01-01

    EAS-i California esinduse töötaja Andrus Viirg tutvustab Eesti ettevõtete võimalusi äritegevuse alustamiseks ja investorite leidmiseks Silicon Valley's. Vt. samas: Skype'i tuules lendavad ettevõtted. San Joses kavandavad kontori avamist Eesti ettevõtted Yoga Intelligence, Myoton ja Solis Biodyne

  3. Synthesis of novel photochromic pyrans via palladium-mediated reactions

    NARCIS (Netherlands)

    Böttcher, Christoph; Zeyat, Gehad; Ahmed, Saleh A.; Irran, Elisabeth; Cordes, Thorben; Elsner, Cord; Zinth, Wolfgang; Rueck-Braun, Karola

    2009-01-01

    Photochromic pyrans for applications in material and life sciences were synthesized via palladium-mediated cyanation, carbonylation and Sonogashira cross-coupling starting from a bromo-substituted naphthopyran and benzopyrans. A novel photoswitchable benzopyran-based ω-amino acid for Fmoc-based soli

  4. Treatment with IL-2 and IL-12 inhibits tumour cell division in SL2 lymphoma

    NARCIS (Netherlands)

    Masztalerz, A; Van Luyn, M; Werner, N; Molema, G; Everse, LA; Den Otter, W

    2004-01-01

    We examined which mechanism plays a dominant role in the rejection of solid SL2 lymphoma treated with locally applied IL-2 and /or IL-12. This treatment resulted in about 80% cures. There was a moderate influx of leukocytes in the tissue surrounding tumours; yet these cells failed to invade the soli

  5. Maleic acid treatment of bioabated corn stover liquors improves cellulose conversion to ethanol

    Science.gov (United States)

    Elimination of inhibitory compounds released during pretreatment of lignocellulose is critical for efficient cellulose conversion and ethanol fermentation. This study examined the effect of bioabated liquor from pretreated corn stover on enzyme hydrolysis of Solka Floc or pretreated corn stover soli...

  6. Effective poroelastic model for one-dimensional wave propagation

    NARCIS (Netherlands)

    Kudarova, A.; Van Dalen, K.N.; Drijkoningen, G.G.

    2013-01-01

    An effective poroelastic model is proposed that describes seismic attenuation and dispersion in periodically layeredmedia. In this model, the layers represent mesoscopic-scale heterogeneities (larger than the grain and pore sizes but smaller than the wavelength) that can occur both in fluid and soli

  7. The Datsuns : külalised eriti kaugelt. Popid poisid Norrast

    Index Scriptorium Estoniae

    2007-01-01

    Uus-Meremaa rockansamblist The Datsuns (www.thedatsuns.com), albumitest "The Datsuns", "Smoke and Mirrors". Norra rockansamblist Apoptygma Berzerk (www.apoptygmaberzerk.de), albumitest "Soli Deo Gloria, "7", "Welcome To Earth", "Harmonizer", "You and Me Against the World", "Sonic Diary". Info festivalist: www.rabarock.delfi.ee / www.rabarock.ee

  8. Electrochemical acidification of milk by whey desalination

    NARCIS (Netherlands)

    Balster, J.; Punt, I.; Stamatialis, D.F.; Lammers, H.; Verver, A.B.; Wessling, M.

    2007-01-01

    We describe a process configuration for the electrochemical acidification of milk using the desalination function and the acid/base production function of a bipolar membrane process. First, the milk is acidified by the acid produced in the bipolar membrane stack. The precipitate is removed by a soli

  9. 75 FR 79803 - Semiannual Agenda of Regulations

    Science.gov (United States)

    2010-12-20

    ... some kinds of flavorings, foods, and beverages. They have begun to use substitutes such as 2,3... the Department's agenda. Hilda L. Solis, Secretary of Labor. Office of Labor-Management Standards...-Management Standards--Completed Actions Regulation Sequence Title Identifier Number Number 397...

  10. Minimaal kinderopvang

    NARCIS (Netherlands)

    S.G. van der Lecq (Fieke)

    2001-01-01

    textabstractWie dacht dat Amerikanen alleen maar hard en lang werken, loopt achter: de balans tussen werk en privé heeft nu de kolommen van de Harvard Business Review gehaald 1. Ook in de VS speelt de discussie tussen werkende ouders en hun kinderloze collega's over de grenzen van de onderlinge soli

  11. Problematiche del copyright della Biblioteca digitale

    OpenAIRE

    Tammaro, Anna Maria

    2006-01-01

    Sono discussi alcuni temi come: differenze del diritto di autore nella biblioteca tradizionale e nella biblioteca digitale, licenze e creative commons, problematiche di autenticità, privacy e deposito legale. Il testo completo della dispensa è accessibile ai soli utenti autorizzati.

  12. Gene Expression Differences between Noccaea caerulescens Ecotypes Help to Identify Candidate Genes for Metal Phytoremediation

    NARCIS (Netherlands)

    Halimaa, P.; Lin, Y.F.; Ahonen, V.H.; Blande, D.; Clemens, S.; Gyenesei, A.; Haikio, E.; Karenlampi, S.O.; Laiho, A.; Aarts, M.G.M.; Pursiheimo, J.P.; Schat, H.; Schmidt, H.; Tuomainen, M.H.; Tervahauta, A.I.

    2014-01-01

    Populations of Noccaea caerulescens show tremendous differences in their capacity to hyperaccumulate and hypertolerate metals. To explore the differences that could contribute to these traits, we undertook SOLiD high-throughput sequencing of the root transcriptomes of three phenotypically well-chara

  13. Crude Oil Stockpiling Needed in China

    Institute of Scientific and Technical Information of China (English)

    Fu Bin

    1997-01-01

    @@ The two oil crises in the 1970's had badly disturbed the economy and the way of life of those oil importing countries, which began to realize the importance of oil stockpiling. With the rapid development of economy, China could no longer satisfy itself soly with the domesticallyproduced oil.

  14. Brasil, estudos pós-coloniais e contracorrentes análogas: entrevista com Ella Shohat e Robert Stam

    NARCIS (Netherlands)

    dos Santos, E.R.S.; Schor, P.

    2013-01-01

    Interview with Ella Shohat and Robert Stam (Portuguese translation). Original version: Brazil is not Traveling Enough”: On Postcolonial Theory and Analogous Counter-Currents (http://www2.let.uu.nl/solis/psc/P/PVOLUMEFOUR/PVOLUMEFOURPAPERS/P4SHOHAT-STAM-INTERVIEW.pdf)

  15. Remote sensing evidence for regolith water vapor sources on Mars

    Science.gov (United States)

    Huguenin, R. L.; Clifford, S. M.

    1982-01-01

    McCord et al. (1977) have presented earth-based photometric imaging data of an event associated with the 1973 dust storm on Mars. The initial dust cloud in Solis Lacus and two regions to the north and south appeared anomalously bright at blue wavelengths. Water frosts, hazes, and/or clouds were identified, and it was suggested that the water responsible for these findings may have originated from Solis Lacus. More recently, a more intensive review of the observational record of Mars was undertaken. Earth-based telescope observations and data from the Mariner and Viking missions have revealed that Solis Lacus has been a center of repeated activity. Persistent activity in the vicinity of Noachis-Hellespontus and in the border regions of Syrtis Major was also discovered. A review of the observations is provided and possible interpretations are discussed. The obtained results appear to support the original proposal that Solis Lacus may be a source of water vapor. Noachis-Hellespontus seems to be a similar vapor source

  16. De novo assembly and characterization of the carrot transcriptome reveals novel genes, new markers, and genetic diversity

    Science.gov (United States)

    Among next generation sequence technologies, platforms such as Illumina and SOLiD produce short reads but with higher coverage and lower cost per sequenced nucleotide than 454 or Sanger. A challenge now is to develop efficient strategies to use short-read length platforms for de novo assembly and ma...

  17. DMPD: Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and controlof anti-tumor activity in primary macrophages. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 16846591 Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and controlof anti-tumor activit...Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and controlof anti-tumor activity... IFN-alpha gene regulation and controlof anti-tumor activity in primary macrophages. Authors Solis M, Goubau

  18. Educación, música y posmodernidad : Constructivismo en la iniciación a la guitarra: diseño, implementación y evaluación de un modelo de enseñanza con apoyo de las tic /

    OpenAIRE

    Navarro Solis, José Luis

    2012-01-01

     tesis que para obtener el grado de Doctorado en Música, presenta Jose Luis Navarro Solis ; asesor Antonio Corona Alcalde, Jesús Tejada Gimémez, Gilles Lavigne168 páginas : ilustracionesDoctorado en Música UNAM, Escuela Nacional de Música, 2012

  19. Combining two technologies for full genome sequencing of human.

    Science.gov (United States)

    Skryabin, K G; Prokhortchouk, E B; Mazur, A M; Boulygina, E S; Tsygankova, S V; Nedoluzhko, A V; Rastorguev, S M; Matveev, V B; Chekanov, N N; D A, Goranskaya; Teslyuk, A B; Gruzdeva, N M; Velikhov, V E; Zaridze, D G; Kovalchuk, M V

    2009-10-01

    At present, the new technologies of DNA sequencing are rapidly developing allowing quick and efficient characterisation of organisms at the level of the genome structure. In this study, the whole genome sequencing of a human (Russian man) was performed using two technologies currently present on the market - Sequencing by Oligonucleotide Ligation and Detection (SOLiD™) (Applied Biosystems) and sequencing technologies of molecular clusters using fluorescently labeled precursors (Illumina). The total number of generated data resulted in 108.3 billion base pairs (60.2 billion from Illumina technology and 48.1 billion from SOLiD technology). Statistics performed on reads generated by GAII and SOLiD showed that they covered 75% and 96% of the genome respectively. Short polymorphic regions were detected with comparable accuracy however, the absolute amount of them revealed by SOLiD was several times less than by GAII. Optimal algorithm for using the latest methods of sequencing was established for the analysis of individual human genomes. The study is the first Russian effort towards whole human genome sequencing.

  20. GLASS-FIBER SELF-MIXING DIODE-LASER DOPPLER-VELOCIMETER

    NARCIS (Netherlands)

    KOELINK, MH; SLOT, M; DEMUL, FFM; GREVE, J; GRAAFF, R; DASSEL, ACM; AARNOUDSE, JG

    1992-01-01

    A novel diode-laser Doppler velocimeter based on self-mixing feedback of Doppler-shifted light in the laser through a single glass fibre is described. The instrument can be applied, for example, to the invasive measurement of blood velocities in blood vessels, and of other industrial fluids and soli

  1. Disruption of cagA, the apoprotein gene of chromoprotein antibiotic C-1027, eliminates holo-antibiotic production, but not the cytotoxic chromophore.

    Science.gov (United States)

    Cui, Zhihui; Wang, Lifei; Wang, Songmei; Li, Guangwei; Hong, Bin

    2009-11-01

    C-1027 is a chromoprotein of the nine-membered enediyne antitumour antibiotic family, comprising apoprotein to stabilize and transport the enediyne chromophore. The disruption of apoprotein gene cagA within the C-1027 biosynthetic gene cluster abolished C-1027 holo-antibiotic production detected by an antibacterial assay, as well as the expression of the apoprotein and C-1027 chromophore extracted following protein precipitation of the culture supernatant. Complementation of the cagA-disrupted mutant AKO with the intact cagA gene restored C-1027 production, suggesting that cagA is indispensable for holo-antibiotic production. Overexpression of cagA in the wild-type strain resulted in a significant increase in C-1027 production as expected. Surprisingly, electrospray ionization (ESI)-MS and ESI-MS/MS analyses suggested that the AKO mutant still produced the C-1027 enediyne chromophore [m/z=844 (M+H)(+)] and its aromatized product [m/z=846 (M+H)(+)]. Consistent with this, the results from gene expression analysis using real-time reverse transcriptase-PCR showed that transcripts of the positive regulator sgcR3 and the structural genes sgcA1, sgcC4, sgcD6 and sgcE were readily detected in the AKO mutant as well as in the wild-type and the complementation strain. These results provided, for the first time, evidence suggesting that the apoprotein of C-1027 is not essential in the self-resistance mechanism for the enediyne chromophore. PMID:19845765

  2. POTEŠKOĆE U NASTAVI SVIRANJA NA UČITELJSKOM STUDIJU

    OpenAIRE

    Šenk, Lidija; Ercegovac Jagnjić, Gordana

    2004-01-01

    U višegodišnjem radu sa studentima koji se pripremaju za zvanje učitelja, nailazimo na cijeli niz poteškoća u realizaciji nastave kolegija Sviranje. Zašto učitelj treba pjevati, svladati vještinu sviranja, znati čitati notne znakove? Zašto razredbenim postupkom nisu provjerene glazbene sposobnosti ako o njima ovisi uspješnost studiranja? Zašto glazbeni sadržaji iziskuju toliko mnogo vremena potrebnoga za njihovo usvajanje i uvježbavanje? Ovo su samo neka od pitanja studenata na početku studij...

  3. Raspodjela HLA-DRB1 gena u hrvatskih bolesnika s artritisom

    OpenAIRE

    Laktašić-Žerjavić, Nadica; Soldo-Jureša, Dragica; Babić-Naglić, Đurđica; Ćurković, Božidar; Potočki, Kristina; Žunec, Renata; Ivanišević, Goran

    2005-01-01

    Reumatoidni artritis (RA) pokazuje povezanost teškog oblika bolesti s određenim HLA-DRB1 alelima. Prema teoriji zajedničkog epitopa prisutnost alela DRB1 lokusa *0401, *0404, *0405, *0408, *0101, *1001 i *1402 povezuje se s destruktivnijim oblikom bolesti, posebice ako su prisutna oba alela. Incidencija RA je 5-7 puta veća u nosioca ovih alela. Cilj rada je bio utvrditi raspodjelu HLA-DRB1 alela u skupini hrvatskih bolesnika s artritisom, i utvrditi najučestaliji podložni gen. Metodom PCR-SSP...

  4. Kategorija brojivosti u hrvatskom jeziku

    OpenAIRE

    Znika, Marija

    2005-01-01

    Kategorija brojivosti promatra se u ovome radu kao na leksičkom značenju imenica utemeljena kategorija. Leksičko značenje imenica može biti dvojako strukturirano: kao jedinično ili kao nejedinično. Temelji se na opreci jedno ≠ mnogo. Kategorija brojivosti ima svoj sadržaj i izraz. Sadržaj kategorije brojivosti čini obilježje [± brojivo] i njegove oznake [+ brojivo] i [- brojivo]. Imenica je brojiva ako je njezin sadržaj pojmljiv kao jediničnost kojoj u opreci stoji mnogost (stol, jabuka). Ime...

  5. Kombinirani bešavni zahvat: fakoemulzifikacija s ugradnjom očne leće i 25-g vitrektomija

    OpenAIRE

    Kovačević, Damir; Mance, Tea; Mišljenović, Tamara

    2008-01-01

    Od otkrića 25-G transkonjuktivne bešavne vitrektomije (25G TSV) u 2002. godini ova metoda je postala popularna u cijelom svijetu. Uz dobar odabir bolesnika ova bešavna tehnika je sigurna za kirurga i minimalno neugodne za bolesnika. Siva mrena razvit će se nakon vitrektomije u svakog bolesnika u dobi iznad 50 godina. Kombinirane operacije sive mrene i vitrektomije treba uzeti u obzir ako se razmišlja o samo jednom kirurškom bešavnom zahvatu koji je za bolesnika jednostavniji i manje bolan. Dv...

  6. ŠUMARSTVO SA I BEZ NAKNADE ZA OPĆEKORISNE FUNKCIJE ŠUMA

    OpenAIRE

    2013-01-01

    Ovih dana ponovo se pokreće rasprava o naknadi za općekorisne funkcije šuma, pa se pitamo da li to znači da se sprema njeno potpuno ukidanje. Ponavljat ćemo se ako opetovano ukazujemo kako je drvo kao sirovina za primarnu i finalnu obradu samo sporedni proizvod potrajnog gospodarenja šumama, a prava i višestruka vrijednost (i do 50 puta) su općekorisne funkcije šuma. Trudimo se ukazati političarima koji donose odluke, ne-biolozima, pa i kvazi biolozima, kako je: zaštita tla od erozije vodom i...

  7. Sintaksa i semantika u suprotan koordinacije i zamjene

    Directory of Open Access Journals (Sweden)

    Pavao Tekavčić

    1978-12-01

    Full Text Available U onome što se obično naziva adverzativnim rečenicama valja razlikovati dva tipa: adverzativne rečenice (u užem smislu, kojima se izriče kontrast (Laura è bella ma è antipatica, i supstitutivne rečenice, kojima se prvi član ukida i zamjenjuje drugim (Laura non è bella bensì ( è brutta. U studiju ovih dvaju tipova rečenica mogu se razlikovati neke sintaktičke i semantičke kategorije: 1 identitet/kontrast, 2 djelomičnost/potpunost, S afirmacija/ negacija svakoga od dvaju članova, 4 pozitivno/negativno (semantičko značenje članova. U članku se studira semantička prihvatljivost rečenica, restrikcije u upotrebi triju glavnih veznika (però, bensì, ma i mogućnost elipse drugoga pomoćnog glagola. Ako prvi član (A nije negiran, moguć je jedino veznik u značenju 'ali', ali samo onda ako se radi o djelomičnom identitetu i djelomičnom kontrastu, dok je isključen ako se izražava potpuni identitet (*Laura è bella pero è bella ili potpuni kontrast (*Laura è bella pero è brutta. Veznik pero u današnjem talijanskom jeziku ima samo značenje 'ali' Ako je prvi član negiran, uz 'ali' moguce je i 'nego' (tal.bensì, ali s razlikom u značenju (Laura non è bella pero è simpatica. I Laura non è bella bensi è simpatica. Veznik 'nego' isključen je samo onda kada se izražava potpuni identitet (jer nema smisla zamjenjivati jedan pojam drugim, ako su identični, dok je u drugim slučajevima - uz veće ili manje razlike, i ev u posebnim slučajevima - prihvatljiv. Dok però znači samo 'ali' a bensì samo 'nego', treći veznik, ma, koji je danas najčešći, pokriva oba ta značenja. Stoga, ako je prvi član negiran, može doći do dvoznačnosti: Laura non è bella, ma è simpatica može značiti da L. nije lijepa nego simpatična (supstitutivna reč. ili da nije lijepa ali  je simpatična (adverzativna reč.. U takvim rečenicama elipsa drugoga pomoćnog glagola postaje distinktivna: dok će se rečenica Laura non è bella

  8. Imigracija u Europi danas: aparthejd ili građanska kohabitacija?

    OpenAIRE

    Suvin, Darko

    2011-01-01

    Članak raspravlja orijentacije koje bi spriječile klizanje prema državama aparthejda. Počinje fenomenologijom masovnih seljenja, te se pita: Jesu li ne-građani ljudi? Koje su granice narodnog suvereniteta? Kakva je to sloboda ako je dobar dio stanovnika nepotpuno slobodan? Završava s pet povezanih aksioma: 1/ pravo na gostoprimstvo (pa zatim i državljanstvo) središnje je ljudsko pravo; 2/ svaka država treba dati svojim stanovnicima maksimalno mogući opseg građanskih prava; 3...

  9. SOCIOBIOLOGIJA I "SEBIČNI GEN" Moral i etika sa stajališta suvremene biologije

    OpenAIRE

    Kešina, Ivan

    2002-01-01

    Sociobiologija, recentna varijanta istraživanja ponašanja na evolucijsko-teoretskom temelju, postavila je sebi zadaću istražiti evolucijske temelje ljudskoga morala. Polazišna joj je točka fenomen ljudskoga altruizma. Iz evolucijsko-teoretske perspektive može altruističko ponašanje nastati i održati se ako pridonosi daljnjoj predaji gena koji to ponašanje kodiraju. Glavni autori, E. O. Wilson i R. Dawkins, nastoje etiku "biologizirati", ne želeći samo objasniti biološke ...

  10. OCJENE I PRIKAZI: Sisak u obrani od Turaka. Izbor građe 1543-1597.

    OpenAIRE

    Barbarić, Josip

    1995-01-01

    Ova knjiga, objavljena prilikom obilježavanja 400-te obljetnice bitke kod Siska, sadrži 444 izvorna dokumenta, objavljena djelomice "in extenso" s regestima ispred izvornog teksta, djelomice samo u regestima (ako je dokument u cjelini već objavljen) tematski grupirana u četiri cjeline: 1) Sisačka tvrđa (troškovi gradnje, oprema i naoružanje, posada, turski sužnji, izvori prihoda za gradnju i uzdržavanje, obnova tvrđe, opatija Porno u Ugarskoj koja je neko vrijeme bila dodijeljena zag...

  11. Postupci liječenja Dens Invaginatusa

    OpenAIRE

    Šutalo, Jozo; Knežević, Alena; Negovetić-Mandić, Višnja; Tarle, Zrinka; Pandurić, Vlatko

    2003-01-01

    Dens invaginatus je razvojna anomalija koja se očituje uvlačenjem cakline i dentina u unutrašnjost krune i korijena. Aberacija se može očitovati u širokome spektru morfoloških varijacija of foramen coecum do manjeg ili većeg uvlačenja u korijen, a ponekad sve do vrška korijena. Suvremenu podjelu anomalije ponudio je Oehlers godine 1957. Prema njoj postoje tri tipa abnormalnosti. Najčešće se otkriva radiografskim pregledom. Ako postoji komunikacija invaginacije s pulpom ili periradikularnim...

  12. Nekilnojamojo turto sektoriaus situacijos strateginis vertinimas

    OpenAIRE

    Janušaitė, Asta

    2014-01-01

    Darbą sudaro trys dalys: Pirmoje dalyje pateikiami verslo šakos strateginio vertinimo modeliai, analizuojami įvairių autorių darbai šia tematika, parenkamas modelis, kuris panaudotas atliekant tyrimą. Antroje darbo dalyje aptariama nekilnojamojo turto sektoriaus situacija Lietuvoje ir užsienyje, pateikiamas išskiriama nagrinėjama verslo šaka pagal ekonominį veiklos rūšių klasifikatorių. Trečioje dalyje pateikiama Kauno miesto nekilnojamojo turto agentūrų veiklos patrauklumo įvertinimo tyrimo ...

  13. ZANEMARUJE LI ŠUMARSKA PRAKSA NAČELA POTRAJNOG (ODRŽIVOG) GOSPODARENJA ŠUMAMA?

    OpenAIRE

    2013-01-01

    U Šumarskome listu br. 7–8/2004. u rubrici Aktualno, pod naslovom "Nekoliko misli u prilog izradbe kodeksa o gospodarenju prirodnim šumama", akademik Dušan Klepac u 10 je sentenci sažeo bitna načela gospodarenja prirodnim šumama. "Šuma je obnovljivi prirodni resurs; ona se može obnoviti ako se njome pravilno gospodari, ukoliko nije poremećen šumski ekosustav", prva je sentenca, a druga: "Pravilno gospodarenje šumama je potrajno gospodarenje, održavajući gospodarske, ekološke i socijalne funkc...

  14. Споредба меѓу „магацинска откопна метода” и „подетажна откопна метода со отворени откопи” за откопување на тенки рудни жици

    OpenAIRE

    Mijalkovski, Stojance

    2010-01-01

    Vo ovoj trud ke se dade sporedba na Magacinskata otkopna metoda i Podetaznata otkopna metoda so otvoreni otkopi za otkopuvanje na tenki rudni zici. Celta na ovaa sporedba e da se vidi koja od ovie dve otkopni metodi bi bila pouspesna ako se primeni za otkopuvanje na tenki rudni zici. Sporedbata na otkopnite metodi ke ja izvrsime preku primer za otkopuvanje na zicno naogjaliste, kade debelinata na zicite se dvizi od 0,1 do 1,5 m, a istite zalegnuvaat pod agol od 40 do 90°. Vo presmetuvanjat...

  15. Značajke i nepravilnosti menstruacijskog ciklusa

    OpenAIRE

    Topalović, Zlatko

    2010-01-01

    Poremećaji menstruacijskog ciklusa najčešći su ginekološki problemi u adolescentno doba. Prva menstruacija (menarche) najočitiji je znak uspostavljanja funkcije jajnika, odnosno osi hipotalamus–hipofiza–jajnici. Ako se prva menstruacija ne pojavi do 16. godine života uz razvijene spolne osobine ili se ne pojavi do 14. godine života uz odsutnost svih spolnih osobina, govorimo o primarnoj amenoreji i treba ispitati njezin uzrok. Povremeni izostanak menstruacije tijekom spolnog sazrijevanja, koj...

  16. SASTAV ODLAGALIŠNOG PLINA NA ODLAGALIŠTU OTPADA PRUDINEC/JAKUŠEVEC

    OpenAIRE

    Vujević, Dinko; Čalopek, Maja; Novosel, Marija; Anić-Vučinić, Aleksandra

    2014-01-01

    Nagli razvoj urbanizacije i industrijalizacije utjecao je na povećanje problema sakupljanja i odlaganja čvrstog otpada. Neadekvatno rješavanje tog problema može negativno utjecati na zdravlje ljudi i kvalitetu čovjekova okoliša. Na velikim odlagalištima komunalnog otpada stvaraju se znatne količine odlagališnog plina što može imati potencijalno velik utjecaj na kakvoću zraka posebno ako se otpad odlaže na neadekvatan način. U okviru ovog rada provedena je analiza sastava odlagališnog plina na...

  17. Energetski izazovi: europska gledišta i francuski odgovori

    OpenAIRE

    GJIDARA, Marc; Britvić Vetma, Bosiljka

    2013-01-01

    Europska unija nastoji poboljšati europsko energetsko tržište na način da bude bolje koordinirano, jer u ujedinjenoj Europi nije više moguće djelovati izdvojeno. Proizvodnja energije iz obnovljivih izvora velikih razmjera zahtijeva međusobno povezano europsko energetsko tržište, ako se žele postići ciljevi utvrđeni programom „Energija i klima“ usvojenim 2009. Cilj je ovog rada utvrditi na koji način utječu pravila europskog prava na pravno normiranje u energetskim pitanjima i borbi protiv glo...

  18. Dendrološka i hortikulturna vrijednost Arboretuma Lisičine

    OpenAIRE

    Idžojtić, Marilena; Zebec, Marko; Poljak, Igor

    2011-01-01

    Prilikom podizanja arboretuma kriteriji su za izbor biljaka drugačiji nego prilikom hortikulturnoga oblikovanja nekoga prostora. Ipak, ako se osim znanstvene i edukativne funkcije vodi računa i o estetskom izgledu, arboretum će u svako doba godine biti prostor ugodan za boravak, a ne samo skupina eksperimentalnih biljaka. U radu je analizirana dendrološka i hortikulturna vrijednost Arboretuma Lisičine. Analiza je prikazana za svako od dvanaest polja u hortikulturnom dijelu, površine oko 9 ha,...

  19. Řídicí jednotka výrobní linky

    OpenAIRE

    Vargovčík, Pavol

    2015-01-01

    V tejto práci som sa venoval vývoju riadiacej jednotky výrobnej linky. Pre jej implementáciu som zvolil vstavané zariadenie Raspberry Pi. Riadiaca jednotka komunikuje s centrálnym administrátorským systémom, ktorý prideľuje jednotlivým riadiacim jednotkám programy. Tieto programy popisujú interakciu s periférnymi zariadeniami, ako aj komunikáciu s databázovým systémom dohľadateľnosti (Traceability).

  20. O ČEMU OVISI KUPOVINA FUNKCIONALNIH PREHRAMBENIH PROIZVODA?

    OpenAIRE

    Kralik, Igor; Kralik, Zlata; Grčević, Manuela

    2016-01-01

    Funkcionalna hrana nova je kategorija u širokom spektru prehrambenih proizvoda, koju potrošači zadnjih godina sve više traže, upravo radi činjenice da imaju određene zdravstvene beneficije. Prema FUFOSE (Functional Food Science in Europe) programu Europske komisije ne postoji točno određena definicija funkcionalne hrane već je uvedena radna definicija prema kojoj se hrana može smatrati funkcionalnom, ako je dokazano da korisno utječe na jednu ili više ciljanih funkcija organizma, pored odgova...

  1. Teško oštećenje bubrežne funkcije u odraslih bolesnika s akutnim trovanjem octenom kiselinom

    OpenAIRE

    Chibishev, Andon; Sikole, Aleksandar; Pereska, Zanina; Chibisheva, Vesna; Simonovska, Natasha; Orovchanec, Nikola

    2013-01-01

    Octena kiselina organska je kiselina s korozivnim svojstvima koja ovise o njezinoj koncentraciji. Ako se unese u koncentracijama iznad 30 %, može teško oštetiti gornji dio gastrointestinalnog sustava i izazvati intravaskularnu hemolizu, što može dovesti do teških oštećenja bubrega i jetara te do diseminirane intravaskularne koagulacije. U ovome retrospektivnom ispitivanju analizirani su slučajevi gutanja octene kiseline prikupljeni u Sveučilišnoj klinici za toksikologiju u Skopju od 1. siječn...

  2. INTERAKCIJA TEŠKIH METALA SA SELENOM U ŽIVOTINJSKOM ORGANIZMU

    OpenAIRE

    Vukšić, Neška; Pavić, Mirela; Đidara, M.; Jurčević, J.; Šperanda, Marcela

    2014-01-01

    Teški metali su prirodni sastojci Zemljine kore, ali zahvaljujući ljudskoj aktivnosti promijenio se njihov geokemijski ciklus i biokemijska aktivnost u čovjeku i životinjskim organizmima. Svaki metal (ili metaloid) može postati kontaminirajući ako se pojavljuje u obliku i koncentraciji koja štetno djeluje na čovjeka ili okoliš. Elementi poput kadmija (Cd), žive (Hg), olova (Pb) i arsena (As) kruže u prirodi u različitim oksidacijskim stanjima. U povišenim količinama mogu biti štetni, a toksič...

  3. Nezavisna astronomska kontrola vanjske geodetske mreže tunela ''Mala Kapela''

    OpenAIRE

    Solarić, Nikola; Špoljarić, Drago

    2005-01-01

    Opisana je automatizirana astronomska kontrola vanjske geodetske mreže tunela "Mala Kapela" i moguće povećanje točnosti proboja tunela, osobito ako su strane za orijentaciju poligonskih vlakova kojima se ulazi u tunel kratke i strme. U dosadašnjim primjenama nezavisne astronomske kontrole geodetskih mreža iznad tunela automatizirano je određivan samo astronomski azimut. Komponente otklona vertikale računane su iz topografskih masa ili geoida, što je manje točno od komponenti određenih astr...

  4. KAKO JE UHIĆEN NAŠ ČOVJEK U DAMASKU?

    OpenAIRE

    Derenčin, Robert

    2015-01-01

    Eli Cohen, „naš čovjek u Damasku”, bio je izraelski obavještajac koji je nekoliko godina iz Damaska u Izrael slao neprocjenjivo važne podatke. Cohen nije bio špijun plejboj koji je trošio Mossadov novac na to da se sprijatelji s visokorangiranim sirijskim službenicima kako bi pribavljao podatke. Eli Cohen bio je skroman vojnik poslan u opasnu misiju na neprijateljski teritorij. Ako je doista napravio barem jednu fatalnu pogrešku zbog koje je uhićen, bio je to neizbježan kraj za čovjeka koji j...

  5. VRTLOG NOVOGA SVIJETA

    OpenAIRE

    Dujmović, Mauro

    2011-01-01

    Aldous Huxley napisao je roman Vrli novi svijet 1932. godine, dok je George Orwell napisao svoj roman 1984. negdje između 1945. i 1948. godine. Izrazit očaj, krajnji pesimizam i besperspektivnost čovjekove sadašnjosti i budućnosti glavne su odlike ovih romana, a upozorenje se odnosi na činjenicu da ako se u tijeku povijesti i razvoja ljudskoga društva nešto ne promijeni, ljudi će širom svijeta izgubiti većinu svojih ljudskih obilježja i postati automati bez duše, a da tog...

  6. Sequencing bias: comparison of different protocols of MicroRNA library construction

    Directory of Open Access Journals (Sweden)

    Tian Geng

    2010-09-01

    Full Text Available Abstract Background MicroRNAs(miRNAs are 18-25 nt small RNAs playing critical roles in many biological processes. The majority of known miRNAs were discovered by conventional cloning and a Sanger sequencing approach. The next-generation sequencing (NGS technologies enable in-depth characterization of the global repertoire of miRNAs, and different protocols for miRNA library construction have been developed. However, the possible bias between the relative expression levels and sequences introduced by different protocols of library preparation have rarely been explored. Results We assessed three different miRNA library preparation protocols, SOLiD, Illumina versions 1 and 1.5, using cloning or SBS sequencing of total RNA samples extracted from skeletal muscles from Hu sheep and Dorper sheep, and then validated 9 miRNAs by qRT-PCR. Our results show that SBS sequencing data highly correlate with Illumina cloning data. The SOLiD data, when compared to Illumina's, indicate more dispersed distribution of length, higher frequency variation for nucleotides near the 3'- and 5'-ends, higher frequency occurrence for reads containing end secondary structure (ESS, and higher frequency for reads that do not map to known miRNAs. qRT-PCR results showed the best correlation with SOLiD cloning data. Fold difference of Hu sheep and Dorper sheep between qRT-PCR result and SBS sequencing data correlated well (r = 0.937, and fold difference of miR-1 and miR-206 among SOLiD cloning data, qRT-PCR and SBS sequencing data was similar. Conclusions The sequencing depth can influence the quantitative measurement of miRNA abundance, but the discrepancy caused by it was not statistically significant as high correlation was observed between Illumina cloning and SBS sequencing data. Bias of length distribution, sequence variation, and ESS was observed between data obtained with the different protocols. SOLiD cloning data differ from Illumina cloning data mainly because of

  7. Comparison of next-generation sequencing systems.

    Science.gov (United States)

    Liu, Lin; Li, Yinhu; Li, Siliang; Hu, Ni; He, Yimin; Pong, Ray; Lin, Danni; Lu, Lihua; Law, Maggie

    2012-01-01

    With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world's biggest sequencing capacity, has multiple NGS systems including 137 HiSeq 2000, 27 SOLiD, one Ion Torrent PGM, one MiSeq, and one 454 sequencer. We have accumulated extensive experience in sample handling, sequencing, and bioinformatics analysis. In this paper, technologies of these systems are reviewed, and first-hand data from extensive experience is summarized and analyzed to discuss the advantages and specifics associated with each sequencing system. At last, applications of NGS are summarized.

  8. Luteibacter rhizovicinus gen. nov., sp nov., a yellow-pigmented gammaproteobacterium isolated from the rhizosphere of barley (Hordeum vulgare L.)

    DEFF Research Database (Denmark)

    Johansen, Jens E.; Binnerup, Svend J.; Kroer, Niels;

    2005-01-01

    at 4-30 degrees C, pH 6-9 and 0-3% (w/v) NaCl. The strains had identical 16S rRNA gene sequences and ERIC (enterobacterial repetitive intergenic consensus sequence) fingerprint profiles, but could be differentiated by their RAPID (random amplified polymorphic DNA) fingerprint patterns. Strain U96(T...... % to Frateuria aurantia DSM 6220(T) and 96 % to Fulvimonas soli LMG 19981(T). Using LJ96(T) DNA as probe, DNA-DNA hybridizations documented the relationship of the three strains to a single species (87.4-98.7% relatedness) and showed less than 30% relatedness to Frateuria aurantia DSM 62207 and Fulvimonas soli...... DSM 14263(T). Rhodanobacter lindaniclasticus LMG 183857 is not extant and the strain not available from any public strain collections, thus DNA-DNA hybridization could not include this strain. On the basis of genotypic and phenotypic characteristics, the three yellow-pigmented strains could also...

  9. Tekstura i kakvoća Kraškog pršuta, dobivenog dimljenjem šunki različitih masa i slanosti

    OpenAIRE

    Andronikov, Darko; Gašperlin, Lea; Polak, Tomaž; Žlender, Božidar

    2013-01-01

    Svrha je ovoga rada bila istražiti utjecaj mase svježe šunke i količine soli dodane tijekom prerade na tehnološke, senzorske i fizikalno-kemijske značajke Kraškog pršuta zaštićenog zemljopisnog podrijetla. Sveukupno 84 uzoraka svježih šunki (pH-vrijednosti od 5,6 do 5,9; izmjerenih 24 h nakon klanja) križane pasmine svinja Landras × Veliki jorkšir podijeljeno je u skupine prema masi na lagane (9,5-10,5 kg) i teške (11,5-13 kg), a prema udjelu soli na normalno i manje slane. Dobivene su 4 skup...

  10. Maximum power analysis of photovoltaic module in Ramadi city

    OpenAIRE

    Majid Shahatha Salim, Jassim Mohammed Najim, Salih Mohammed Salih

    2013-01-01

    Performance of photovoltaic (PV) module is greatly dependent on the solar irradiance, operating temperature, and shading. Solar irradiance can have a significant impact on power output of PV module and energy yield. In this paper, a maximum PV power which can be obtain in Ramadi city (100km west of Baghdad) is practically analyzed. The analysis is based on real irradiance values obtained as the first time by using Soly2 sun tracker device. Proper and adequate information on solar radiation an...

  11. miRSeqNovel

    DEFF Research Database (Denmark)

    Qian, Kui; Auvinen, Eeva; Greco, Dario;

    2012-01-01

    We present miRSeqNovel, an R based workflow for miRNA sequencing data analysis. miRSeqNovel can process both colorspace (SOLiD) and basespace (Illumina/Solexa) data by different mapping algorithms. It finds differentially expressed miRNAs and gives conservative prediction of novel miRNA candidates...... with customized parameters. miRSeqNovel is freely available at http://sourceforge.net/projects/mirseq/files....

  12. SINTEZA ( R )-1-AMINOINDANA

    OpenAIRE

    Prudič, Darja

    2014-01-01

    (R)-1-aminoindan je pomemben intermediat za sintezo zdravilne farmacevtske učinkovine rasagilina, (R)(+)-N-propargil-1-aminoindana, oz. njegove soli. Zdravila osnovana na navedeni učinkovini se uporabljajo za zdravljenje indikacij Parkinsonove bolezni, motenj spomina, demence tipa Alzheimerjeve bolezni, depresije in hiperaktivnega sindroma pri otrocih. Magistrska naloga temelji na razvoju učinkovite sinteze (R)-1-aminoindana in preveritvi ter potrditvi ustreznosti tega ključnega intermediata ...

  13. Solid state proton conductors properties and applications in fuel cells

    CERN Document Server

    Knauth, Philippe

    2012-01-01

    Proton conduction can be found in many different solid materials, from organic polymers at room temperature to inorganic oxides at high temperature. Solid state proton conductors are of central interest for many technological innovations, including hydrogen and humidity sensors, membranes for water electrolyzers and, most importantly, for high-efficiency electrochemical energy conversion in fuel cells. Focusing on fundamentals and physico-chemical properties of solid state proton conductors, topics covered include: Morphology and Structure of Solid Acids Diffusion in Soli

  14. Analýza výrobního procesu vybraného produktu ve farmaceutickém průmyslu

    OpenAIRE

    Pospíšilová, Lucie

    2013-01-01

    Bachelor thesis utilizes lean tools to improve manufacturing process and suggest effective recommendations. The aim of this thesis is to use process mapping to identify possible wastes. The first part contains characterization of the pharmaceutical industry, market definition and classification of a company Zentiva Group a.s. The second part describes terms of lean manufacturing and analytic process tools used in the practical part. Process tools are applied to a manufacturing process of soli...

  15. Babies, bodies and entitlement: gendered aspects of access to citizenship in the Republic of Ireland

    OpenAIRE

    Garner, Steve

    2007-01-01

    Since the mid-1990s, automatic citizenship for children born in the Republic has been a source of growing debate against a backdrop of increasing immigration and the peace process. In June 2004, the debate culminated in a referendum, opening the way to a constitutional amendment that attaches residence qualifications to the hitherto unfettered entitlement to citizenship available through ius soli. Arguments for the amendment were couched in terms of a threat posed by Third World women having ...

  16. Biblioteca digitale: visione e realtà

    OpenAIRE

    Tammaro, Anna Maria

    2006-01-01

    Sono discussi alcuni temi come: perché la biblioteca digitale richiede una definizione diversa dalla biblioteca tradizionale, quale è il ruolo delle biblioteche digitali, la necessaria cooperazione delle biblioteche digitali verso il sistema delle biblioteche digitali, l’abbattimento di confini tra professioni finora separate, come gli archivisti, i curatori dei musei, i bibliotecari. Il testo completo della dispensa è accessibile ai soli utenti autorizzati.

  17. Citizen or Subordinate: Permutations of Belonging in the United States and the Dominican Republic

    OpenAIRE

    Shaina Aber; Mary Small

    2013-01-01

    The Dominican Republic and the United States have both experienced tensions arising from migratory flows from poorer, less stable neighbors. Until recently, both countries had constitutions which conferred citizenship by birth with very limited exceptions. Despite these similarities, their respective discourses around jus soli citizenship, particularly for the children of unauthorized migrants from the poorer neighboring countries, have manifested in different ways. The identity of the United...

  18. Case for diagnosis Caso para diagnóstico

    OpenAIRE

    Felipe Maurício Soeiro Sampaio; Fabrício Tinoco Lourenço; Daniel Lago Obadia; Leninha Valério do Nascimento

    2012-01-01

    Male patient, 28 years old, presented with an asymptomatic yellowish erythematous papule on his right thigh. Excisional biopsy was performed for histopathological examination of the lesion. Multinucleated cells (Touton giant cells) were observed. S100 immunohistochemistry was negative for CD1a and positive for CD4 and CD68. Based on clinical and histopathological findings associated with immunohistochemistry, we concluded that it was a case of adult-type xanthogranuloma. Because it was a soli...

  19. Aplicação de cinzas residuais e de fibra de sisal na produção de argamassas e concretos: Revisão

    OpenAIRE

    Indara Soto Izquierdo; Marcio Antonio Ramalho

    2014-01-01

    The industrial development linked to the growing addiction to con - sumption, obsession with fossil fuels, and general desire for short term profit, cause a certain insensitivity to more sustainable alternatives. The construction industry is a sector in which the incorporation of various types of waste presents as favorable. Three types of waste with different characteristics and origins have proved attractive and advantageous for many developing countries. They are incinerated municipal soli...

  20. Ca3Co4O9+δ, a growing potential SOFC cathode material: impact of the layer composition and thickness on the electrochemical properties

    NARCIS (Netherlands)

    Rolle, A.; Abbas, H.A.A.; Huo, D.; Capoen, E.; Mentré, O.; Vannier, R.N.; Daviero-Minaud, S.; Boukamp, B.A.

    2016-01-01

    The thermoelectric material Ca3Co4O9 + δ (CCO), with an electronic conductivity of σe = 240 S·cm− 1 at 650 °C and a good chemical and mechanical compatibility with the standard Ce0.9Gd0.1O1.95 electrolyte (CGO, TEC: 9–10 · 10− 6 K− 1), was recently identified as a potential cathode material for soli

  1. Comparison of Next-Generation Sequencing Systems

    OpenAIRE

    Lin Liu; Yinhu Li; Siliang Li; Ni Hu; Yimin He; Ray Pong; Danni Lin; Lihua Lu; Maggie Law

    2012-01-01

    With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world’s ...

  2. Massively Parallel Sequencing Approaches for Characterization of Structural Variation

    OpenAIRE

    Koboldt, Daniel C.; Larson, David E.; Chen, Ken; Ding, Li; Wilson, Richard K.

    2012-01-01

    The emergence of next-generation sequencing (NGS) technologies offers an incredible opportunity to comprehensively study DNA sequence variation in human genomes. Commercially available platforms from Roche (454), Illumina (Genome Analyzer and Hiseq 2000), and Applied Biosystems (SOLiD) have the capability to completely sequence individual genomes to high levels of coverage. NGS data is particularly advantageous for the study of structural variation (SV) because it offers the sensitivity to de...

  3. Integrated solid waste management in megacities

    OpenAIRE

    M.A. Abdoli; Rezaee, M.; H. Hasanian

    2016-01-01

    Rapid urbanization and industrialization, population growth and economic growth in developing countries make management of municipal solid waste more complex comparing with developed countries. Furthermore, the conventional municipal solid waste management approach often is reductionists, not tailored to handle complexity. Therefore, the need to a comprehensive and multi-disciplinary approach regarding the municipal solid waste management problems is increasing. The concept of integrated soli...

  4. Quantitative trait loci pyramiding for fruit quality traits in tomato

    OpenAIRE

    Sacco, Adriana; Di Matteo, Antonio; Lombardi, Nadia; Trotta, Nikita; Punzo, Biancavaleria; Mari, Angela; Barone, Amalia

    2012-01-01

    Fruit quality is a major focus for most conventional and innovative tomato breeding strategies, with particular attention being paid to fruit antioxidant compounds. Tomatoes represent a major contribution to dietary nutrition worldwide and a reservoir of diverse antioxidant molecules. In a previous study, we identified two Solanum pennellii introgression lines (IL7-3 and IL12-4) harbouring quantitative trait loci (QTL) that increase the content of ascorbic acid (AsA), phenols and soluble soli...

  5. Preparation and evaluation of multiple-unit solid oral dosage forms containing chemical permeation enhancing agents / Elmarie Kleynhans

    OpenAIRE

    Kleynhans, Elmarie

    2014-01-01

    The most popular and convenient route of drug administration remains the oral route, however, protein and peptide drugs such as insulin have poor membrane permeability and stability in the gastrointestinal tract. Absorption enhancers can be added to drug delivery systems to overcome the epithelial cell membrane permeability problem. Although previous studies have shown that aloe leaf materials improve the transport of drugs across intestinal epithelia, their performance in soli...

  6. Drug: D03940 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D03940 Drug Eculizumab (genetical recombination) (JAN); Eculizumab (USAN/INN); Soli...gic organisms and parasites 63 Biological preparations 639 Miscellaneous 6399 Others D03940 Eculizumab (genetica...IMMUNOSUPPRESSANTS L04AA Selective immunosuppressants L04AA25 Eculizumab D03940 Eculizumab (genetical recomb...08310] Others Complement system C5 [HSA:727] [KO:K03994] Eculizumab [ATC:L04AA25] D03940 Eculizumab (genetica

  7. Posouzení účinnosti čištění odpadních vod pomocí testů ekotoxicity

    OpenAIRE

    Urminská, Barbora

    2015-01-01

    Možnosti kontroly znečistenia životného prostredia sa ustavične zlepšujú a začína sa klásť dôraz na pokročilejšie metódy čistenia odpadových vôd, ako i na dôkladnejšiu kontrolu obsahu kontaminantov vo vyčistených vodách. Vďaka tomu sa okrem chemických analýz stávajú relevantnými tiež ekotoxikologické biotesty ako prostriedky hodnotenia biologických účinkov vypúšťaných vôd na ekosystémy. Žiadna čistiareň nečistí odpadové vody dokonale, iba znižuje znečistenie na prijateľnú mieru. Preto je dôle...

  8. Methionine sulfoxide reductase A affects β-amyloid solubility and mitochondrial function in a mouse model of Alzheimer's disease.

    Science.gov (United States)

    Moskovitz, Jackob; Du, Fang; Bowman, Connor F; Yan, Shirley S

    2016-03-15

    Accumulation of oxidized proteins, and especially β-amyloid (Aβ), is thought to be one of the common causes of Alzheimer's disease (AD). The current studies determine the effect of an in vivo methionine sulfoxidation of Aβ through ablation of the methionine sulfoxide reductase A (MsrA) in a mouse model of AD, a mouse that overexpresses amyloid precursor protein (APP) and Aβ in neurons. Lack of MsrA fosters the formation of methionine sulfoxide in proteins, and thus its ablation in the AD-mouse model will increase the formation of methionine sulfoxide in Aβ. Indeed, the novel MsrA-deficient APP mice (APP(+)/MsrAKO) exhibited higher levels of soluble Aβ in brain compared with APP(+) mice. Furthermore, mitochondrial respiration and the activity of cytochrome c oxidase were compromised in the APP(+)/MsrAKO compared with control mice. These results suggest that lower MsrA activity modifies Aβ solubility properties and causes mitochondrial dysfunction, and augmenting its activity may be beneficial in delaying AD progression.

  9. Library

    OpenAIRE

    Vaňková, Jana

    2014-01-01

    Diplomová práca rieši realizáciu a vypracovanie projektovej dokumentácie objektu novostavby knižnice. Navrhovaný objekt bude slúžiť ako verejná mestská knižnica pre širokú verejnosť s počtom knižných jednotiek 102 200. Nachádza sa tu oddelenie pre dospelých, ako aj pre deti, odborná literatúra, audiovizuálne oddelenie, počítačové učebne a študovne. V objekte sú umiestnené prevádzky kaviarne, galérie a univerzálna prednášková sála. Objekt má tri nadzemné podlažia a jedno podzemné podlažie, kde...

  10. Pharmacological Analysis of Vorinostat Analogues as Potential Anti-tumor Agents Targeting Human Histone Deacetylases: an Epigenetic Treatment Stratagem for Cancers.

    Science.gov (United States)

    Praseetha, Sugathan; Bandaru, Srinivas; Nayarisseri, Anuraj; Sureshkumar, Sivanpillai

    2016-01-01

    Alteration of the acetylation status of chromatin and other non-histone proteins by HDAC inhibitors has evolved as an excellent epigenetic strategy in treatment of cancers. The present study was sought to identify compounds with positive pharmacological profiles targeting HDAC1. Analogues of Vorinostat synthesized by Cai et al, 2015 formed the test compounds for the present pharmacological evaluation. Hydroxamte analogue 6H showed superior pharmacological profile in comparison to all the compounds in the analogue dataset owing to its better electrostatic interactions and hydrogen bonding patterns. In order to identify compounds with even better high affinity and pharmacological profile than 6H and Vorinostat, virtual screening was performed. A total of 83 compounds similar to Vorinostat and 154 compounds akin to analogue 6H were retrieved. SCHEMBL15675695 (PubCid: 15739209) and AKOS019005527 (PubCid: 80442147) similar to Vorinostat and 6H, were the best docked compounds among the virtually screened compounds. However, in spite of having good affinity, none of the virtually screened compounds had better affinity than that of 6H. In addition SCHEMBL15675695 was predicted to be a carcinogen while AKOS019005527 is Ames toxic. From, our extensive analysis involving binding affinity analysis, ADMET properties predictions and pharmacophoric mappings, we report Vorinostat hydroxamate analogue 6H to be a potential candidate for HDAC inhibition in treatment of cancers through an epigenetic strategy. PMID:27039807

  11. Vliv kogenerace na energetiku a trh s elektrickou energií v ČR

    OpenAIRE

    Dudáš, Michal

    2014-01-01

    Hlavným cieľom diplomovej práce je zistiť vplyv možného budúceho rozvoja kogeneračných technológií na energetický trh v ČR hlavne s väzbou na historický vývoj vo fotovoltaike v ČR a dotačnou politikou štátu. V práci sú navrhnuté tri scenáre nárastu inštalovaného výkonu a výpočet navýšenia príspevku na OZE, ako aj celkové vyhodnotenie. Práca ďalej spracúva rešerš súčasného stavu kombinovanej výroby elektriny a tepla v Českej republike, Európskej únii a USA, ako aj legislatívny rámec problemati...

  12. Seasonal Work in Hungary in the Light of Seasonal Work Directive / Sezónna Práca V Maďarsku Vo Svetle Smernice Pre Sezónnu Prácu

    Directory of Open Access Journals (Sweden)

    Ács Vera

    2015-06-01

    Full Text Available Smernica tykajuca sa sezonnej prace tvori sučasť sektorovej imigračnej politiky EU, nakoľko po nekvalifi kovanej pracovnej sile v ramci EU jestvuje permanenty dopyt. Smernica zavadza spoločne normy so zjednodušenymi postupmi pre vstup a lepšie vyhliadky na navrat v nasledujucej sezone v odvetviach, ktore su označene ako sezonne - v najvačšej miere je to poľnohospodarstvo, zahradnictvo a cestovny ruch - kde sa vo veľkej miere uplatňuju štatni prislušnici tretich krajin. Ako ochranu proti hospodarskemu a socialnemu vykorisťovaniu, smernica zavadza prava, na ktore maju narok sezonni pracovnici z tretich krajin počas ich pobytu, a ktore pred nekalou suťažou chrania tiež občanov EU pracujucih sezonne. V sučasnosti Maďarsko nie je cieľovou krajinou pre sezonne prace. Smernica nestanovuje len povinnosti pre členske krajiny, ale poskytuje tiež šancu na zatraktivnenie tychto krajin pre dočasnu migraciu

  13. What are you waiting for?

    CERN Multimedia

    CERN video productions

    2012-01-01

    Video artists: Sandor Tokai, Gyorgy Ujhazi, Akos Ancsin, Otto Varga (students / video artists at the University of Debrecen, Hungary and highschool students – refer to themselves as the Lightscribblers) Soundtrack: Akos Szilagyi (student and composer from the University of Szeged, Hungary, who goes under the name of Rise of Day) Summary: This video was made as part of the outreach needs for the European Commission Marie Curie Actions of which CERN is a major beneficiary. The emphasis is on reaching out to communicate science and advertise the projects to the general public. The specific aim of this video is to inspire university graduates to start a research career in science and technology. As CERN is such a major beneficiary of funding for the Marie Curie well as to reach the general public who are not aware of CERN and Marie Curie opportunities. Recent experience has shown that there is a lack of good candidates and this video is intended to create an awareness among graduates that CERN and the Marie Cur...

  14. Comparison of sequencing platforms for single nucleotide variant calls in a human sample.

    Science.gov (United States)

    Ratan, Aakrosh; Miller, Webb; Guillory, Joseph; Stinson, Jeremy; Seshagiri, Somasekar; Schuster, Stephan C

    2013-01-01

    Next-generation sequencings platforms coupled with advanced bioinformatic tools enable re-sequencing of the human genome at high-speed and large cost savings. We compare sequencing platforms from Roche/454(GS FLX), Illumina/HiSeq (HiSeq 2000), and Life Technologies/SOLiD (SOLiD 3 ECC) for their ability to identify single nucleotide substitutions in whole genome sequences from the same human sample. We report on significant GC-related bias observed in the data sequenced on Illumina and SOLiD platforms. The differences in the variant calls were investigated with regards to coverage, and sequencing error. Some of the variants called by only one or two of the platforms were experimentally tested using mass spectrometry; a method that is independent of DNA sequencing. We establish several causes why variants remained unreported, specific to each platform. We report the indel called using the three sequencing technologies and from the obtained results we conclude that sequencing human genomes with more than a single platform and multiple libraries is beneficial when high level of accuracy is required.

  15. Citizen or Subordinate: Permutations of Belonging in the United States and the Dominican Republic

    Directory of Open Access Journals (Sweden)

    Shaina Aber

    2013-09-01

    Full Text Available The Dominican Republic and the United States have both experienced tensions arising from migratory flows from poorer, less stable neighbors. Until recently, both countries had constitutions which conferred citizenship by birth with very limited exceptions. Despite these similarities, their respective discourses around jus soli citizenship, particularly for the children of unauthorized migrants from the poorer neighboring countries, have manifested in different ways. The identity of the United States as a nation of immigrants has limited the success of campaigns to revoke jus soli citizenship for the children of unauthorized immigrants, but the persistent articulation of this idea as a response to illegal migration has shifted the parameters of the immigration debate. In the Dominican Republic, the historical construction of national identity and anti-Haitian discourse has led to an evolution in Dominican law which codifies already established practices that deny citizenship to children of Haitian migrants. In both cases, movements that support more inclusive understandings of societal belonging, like the DREAMers in the United States and youth movements in the Dominican Republic, may offer the most effective way of protecting universal jus soli citizenship regimes. 

  16. Polyphenolic compounds and essential oil analysis of selected species of the genus Thymus / Analýza fenolových zložiek a analýza silice vybraných druhov rodu Thymus

    Directory of Open Access Journals (Sweden)

    Kameníková M.

    2015-06-01

    Full Text Available Predložena praca bola zamerana na stanovenie obsahu vybranych fenolovych zlučenin (celkove fenoly, triesloviny, flavonoidy a antokyany troch roznych druhov rodu Thymus (T. pulegioides, T. pannonicus, T. praecox s rozdielnym povodom (Českomoravska vrchovina, Křivoklat a Považsky Inovec. Na stanovenie tychto zložiek boli využite spektrofotometricke metody Europskeho liekopisu, 8. vydanie. Zaroveň bol stanoveny aj obsah silice a bola vykonana analyza jej zložiek. Obsah celkovych polyfenolov bol stanoveny spektrofotometrickou metodou s využitim Folin-Ciocalteovho činidla. Obsah kolisal od 3.87 % do 8,86 %. Obsah trieslovin bol realizovany na zaklade predošleho stanovenia obsahu celkovych fenolov, a to adsorpciou trieslovin na kožny prašok. Ich obsah sa pohyboval v rozmedzi 1,96 % do 5,65 %. Na stanovenie obsahu flavonoidov bola využita spektrofotometricka metoda s chloridom hlinitym. Obsah flavonoidov vyjadrenych ako luteolin-7-O-glukozid (λ = 392 nm kolisal od 0,59 % do 1,52 % a obsah flavonoidov vyjadrenych ako rutin (λ = 420 nm sa pohyboval v rozmedzi od 0,41 % do 1,12 %. Antokyany zaberaju v ramci fenolovych zložiek rodu Thymus len minimalny podiel. V našich vzorkach bol obsah antokyanov vyjadreny ako cyanidin-3- O-glukozid a kolisal od 0,02 % do 0,1 %. Na stanovenie obsahu silice bola využita metoda Europskeho liekopisu, 8.vydanie. Obsah silice sa pohyboval v rozmedzi od 0,2 % do 0,75 %. Na analyzu silice bola nasledne využita metoda plynovej chromatografie s hmotnostnou spektrometriou. Na zaklade tejto analyzy bola zistena pritomnosť troch chemotypov silice, a to tymoloveho, karvakroloveho a linalooloveho chemotypu. Vďaka variaciami chemickeho zloženia silice, bolo možne pozorovať vzťah medzi pritomnosťou určiteho chemotypu a povodom vzorky. Rozdiely v stanoveni obsahu flavonoidov nepreukazali žiadny vyrazny vzťah k lokalite povodu.

  17. Hlf is a genetic modifier of epilepsy caused by voltage-gated sodium channel mutations.

    Science.gov (United States)

    Hawkins, Nicole A; Kearney, Jennifer A

    2016-01-01

    Mutations in voltage-gated sodium channel genes cause several types of human epilepsies. Often, individuals with the same sodium channel mutation exhibit diverse phenotypes. This suggests that factors beyond the primary mutation influence disease severity, including genetic modifiers. Mouse epilepsy models with voltage-gated sodium channel mutations exhibit strain-dependent phenotype variability, supporting a contribution of genetic modifiers in epilepsy. The Scn2a(Q54) (Q54) mouse model has a strain-dependent epilepsy phenotype. Q54 mice on the C57BL/6J (B6) strain exhibit delayed seizure onset and improved survival compared to [B6xSJL/J]F1.Q54 mice. We previously mapped two dominant modifier loci that influence Q54 seizure susceptibility and identified Hlf (hepatic leukemia factor) as a candidate modifier gene at one locus. Hlf and other PAR bZIP transcription factors had previously been associated with spontaneous seizures in mice thought to be caused by down-regulation of the pyridoxine pathway. An Hlf targeted knockout mouse model was used to evaluate the effect of Hlf deletion on Q54 phenotype severity. Hlf(KO/KO);Q54 double mutant mice exhibited elevated frequency and reduced survival compared to Q54 controls. To determine if direct modulation of the pyridoxine pathway could alter the Q54 phenotype, mice were maintained on a pyridoxine-deficient diet for 6 weeks. Dietary pyridoxine deficiency resulted in elevated seizure frequency and decreased survival in Q54 mice compared to control diet. To determine if Hlf could modify other epilepsies, Hlf(KO/+) mice were crossed with the Scn1a(KO/+) Dravet syndrome mouse model to examine the effect on premature lethality. Hlf(KO/+);Scn1a(KO/+) offspring exhibited decreased survival compared to Scn1a(KO/+) controls. Together these results demonstrate that Hlf is a genetic modifier of epilepsy caused by voltage-gated sodium channel mutations and that modulation of the pyridoxine pathway can also influence phenotype

  18. Determination of gamma radiation shielding characteristics of some tropical woods

    International Nuclear Information System (INIS)

    This study compares the shielding characteristics of twenty-two tropical woods by using gamma scintillation detection method. Woods sourced are Anogeisus Leiocarpus(Ayin), Nesogordonia Papverifera(Oro), Entandrophragma Microphyllum(Anunje), Brachystagia Eurycoma(Ako), Cassia Alata(Asunrun), Afzelia Africana(Apa-Igbo), Khaya Grandifoliala(Gedu), Piptadenistrum Africana(Agbonyin), Nanclea Diderrehii(Opepe), Khaya Ivorensis(Oganwo), Chlorophora Excelsa(Iroko), Masonia altissima(Odogi), Entandrophragma Angolense(Ijebo), Altium Sativum(Ayo), Albizia Zygia(Ayunre), Terminalia Superba(Afara), Cordia Millenii(Omo), Melania(Melania), Pycnanthus Angolensis(Akomu), Triplochitons Scleroxylon(Arere), Pine(Pine), Ceiba Pentradra(Araba). The intensities of the emergent radiation were measured, when each of these woods were placed between a scintillation detector and a standard radioactive source. Analysis of result obtained shows an appreciable evidence of radiation attenuation due to the changes in the chemical composition of the woods and the dependence of the attenuation coefficient on energy and densities of these woods. The descending order of attenuation coefficient determined are; Ayin, Oro, Anuje, Ako, Asunrun, Apa-igbo Gedu, Agbonyin, Opepe Oganwo, Iroko Odogi , Ayo, Ayunre, Afara, Omo, Melania, Akomu, Arere, Pine, Araba. For a constant energy of 0.101MeV, the attenuation coefficient are 0.190cm-1, 0.165cm-1, 0.163cm-1, 0.156cm-1, 0.149cm-1, 0.143cm-1, 0.133cm-1, 0.132cm-1, 0.127cm-1, 0.124cm-1, 0.085cm-1, 0.123cm-1, 0.122cm-1, 0.113cm-1, 0.101cm-1, 0.088cm-1, 0.087cm-1, 0.086cm-1, 0.082cm-1 respectively. The wood in descending order of dependence of attenuation coefficient on density are: Ayin,Oro, Anunje,Ako,Asunrun,Apa-Igbo, Gedu, Agbonyin, Opepe, Oganwo, Iroko, Odogi, Ijebo, Ayo, Ayunre, Afara, Omo, Melania, Akomu, Arere, Pine and Araba. The half value layer shows the thickness at various energy regions.

  19. Návrh podnikatelského plánu a strategie rozvoje podniku

    OpenAIRE

    Hliničan, Marek

    2013-01-01

    Predmetom tejto diplomovej práce je vypracovanie podnikateľského zámeru pre založenie malej firmy podnikajúcej v oblasti informačných technológií. V teoretickej časti práce sú obsiahnuté teoretické východiská práce a vysvetlenie základných pojmov a konkrétnych metód. Výstupom analytickej časti práce je PESTEL a SWOT analýza spoločnosti a jej okolia. Práca by mala slúžiť ako pomocné vodítko pre zostavenie kvalitného podnikateľského zámeru z teoretického hľadiska. The subject of this thesis ...

  20. Moderní hermeticky těsná osvětlovací jednotka do extrémních podmínek

    OpenAIRE

    Šerík, Martin

    2011-01-01

    Práca sa zaoberá rešeršou dostupných riešení pri výbere komponentov pre hermeticky tesnú osvetľovaciu jednotku do extrémnych podmienok a jej zostrojením. Po porovnaní jednotlivých parametrov sa vybrali ako zdroj energie 4 AAA batérie eneloop od firmy Sanyo, zdrojom svetla je vysokosvietivá LED CREE XP-G a riadiacou jednotkou mikrokontrolér ATtiny24 od firmy Atmel. Interakcia s užívateľom je realizovaná pomocou bezkontaktného kapacitného ovládania a nabíjanie svietidla prebieha bezkontaktným s...

  1. Senzorické zařízení s Windows 10 IoT Core

    OpenAIRE

    Vaľuš, Dávid

    2016-01-01

    Táto práca sa zaoberá inštaláciou systému Windows 10 IoT Core na mikropočítač Raspberry Pi 2 a vytvorením AllJoyn aplikácie na meranie svetelnosti, teploty a vlhkosti vzduchu cez bluetooth rozhranie. V teoretickej časti sú popísané technológie ako M2M (Machine to Machine), IoT (Internet of Things), ich aktuálne nasadenie, vývoj do budúcnosti a platforma AllJoyn. Ďalej je v teoretickej častí porovnanie operačných systémov dostupných pre Raspberry Pi 2. V praktickej časti som sa venoval samotne...

  2. Lietuvos ir Europos Sąjungos socialinių – ekonominių modelių lyginamoji analizė: darbo rinkos reguliavimas

    OpenAIRE

    Gliosaitė, Kristina

    2007-01-01

    Pastarąjį dešimtmetį dėl vis didėjančio globalizacijos tempo vienu iš aktualiausių debatų ekonomikos srityje tapo konkurencingumas. Jis dažnai siejamas su valstybių ekonominio potencialo vystymu, kurį įtakoja ne tik atskiros ekonomikos šakos ar įmonės, tačiau ir valstybinis sektorius, pasitelkdamas reguliacinę politiką. Ji sukuria tam tikrą veikimo rėmą – nustato privačių veikėjų elgesio taisykles, ir tokiu būdu įtakoja atskirų rinkų, tokių kaip prekių, kapitalo, darbo jėgos funkcionavimą bei...

  3. Optimalizácia monitorovania sieťovej prevádzky

    Directory of Open Access Journals (Sweden)

    František Jakab

    2013-06-01

    Full Text Available Tento príspevok sa zaoberá otvorenými problémami vyskytujúcimi sa pri pasívnom prístupe merania sieťových charakteristík. Opisuje najpoužívanejšie prístupy merania sieťových parametrov ako aj charakteristiky, ktoré sa pri monitorovaní najčastejšie sledujú. Hlavným cieľom tohto príspevku je predstavenie konceptuálneho návrhu riešenia opísaných problémov, ktorý by sa mal docieliť automatizovaným prispôsobením exportu záznamov o tokoch prevádzky k aktuálnemu stavu siete.

  4. Návrh transformační stanice pro vyvedení výkonu z kogenerační jednotky

    OpenAIRE

    Pastorek, Jaroslav

    2012-01-01

    Diplomová práca sa zaoberá problematikou návrhu transformačnej stanice pre vyvedenie výkonu z kogeneračnej jednotky o elektrickom výkone do 1 MW. V práci je vypracovaný postup navrhovania jednotlivých zariadení ako je transformátor s menovitým prevodom 22/0,4 kV, rozvádzač VN a rozvádzač NN. Pri správnom výbere transformátoru sa okrem technických parametrov posudzuje najmä jeho ekonomická výhodnosť, ktorá vychádza z jeho nákupnej ceny a nákladov na straty pri danej prevádzke transformátora. N...

  5. Teisės į privatų gyvenimą gynimas baudžiamosios teisės požiūriu

    OpenAIRE

    Stanislauskienė, Edita

    2010-01-01

    Santrauka Lietuvos Respublikos Konstitucijoje įrašytas reikalavimas gerbti privatų gyvenimą reiškia nuostatą be reikalo neapriboti asmens teisių. Egzistuojant galimybei įstatymų leidėjui pasirinkti, kurios teisės šakos normomis apibrėžti tam tikrus teisės pažeidimus ir kokias sankcijas už juos nustatyti, asmuo privatumą gali ginti pasitelkęs civilinės, administracinės ar baudžiamosios teisės normas. Galimais privataus gyvenimo pažeidimais baudžiamosios teisės kontekste laikomi nepagrįstas įsi...

  6. Restauracije mliječnih zuba cirkonijskim krunicama kod djece s ranim karijesom

    OpenAIRE

    Karaca, Serhat; Ozbay, Gizem; Kargul, Betul

    2013-01-01

    Rani dječji karijes (ECC) razarajući je oblik truljenja zuba koji pogađa predškolsku djecu. Obično se pojavljuje na mliječnim maksilarnim sjekutićima dojenčadi i vrlo male djece. Ti su zubi vrlo važni za fizički izgled, pa ako nestane njihova struktura to ne utječe samo na estetiku nego kompromitira i žvakanje, a uzrokuje i teškoće u socijalnoj i psihološkoj prilagodbi djeteta. Estetska restauracija mliječnih sjekutića može se obaviti različitim materijalima, primjerice, gotovim cirkonij...

  7. Detekce překážek

    OpenAIRE

    Hradiský, Marek

    2016-01-01

    Diplomová práca sa zaoberá návrhom a popisom realizácie snímania obrazu pomocou minipočítača Raspberry PI a moţnosťou detekcie objektu v obraze. V práci je popísaný minipočítač Raspberry PI ako aj snímanie obrazu modulom PiCamera a princíp spracovania obrazu prostredníctvom OpenCV. Diploma thesis deals with design and the processing of video captured by Raspberry PI and the possibility of object detection in video. In this thesis is described Raspberry PI as well as video capturing by PiCa...

  8. Keynesov animal spirit. Filozofski aspekti učenja Johna Maynarda Keynesa o uzrocima ekonomskih kriza i opravdanju državne intervencije

    OpenAIRE

    Dragojević Mijatović, Aneli

    2013-01-01

    Klasična i neoklasična ekonomska misao, na koju se naslanja današnji neoliberalni princip, poima slobodno tržište kao savršeni mehanizam koji je, ako se i poremeti, sposoban samostalno se vratiti u ravnotežu. Britanski ekonomist John Maynard Keynes još za Velike Depresije 1929. uočava da ne postoji jamstvo za takvo što jer tržište nije zbroj racionalnih individua. Prema Keynesu, čovjek djeluje prema osjećaju, slučaju i hiru, kao iracionalnim porivima koji su, međutim, u epistemički ograničeni...

  9. Didelio meistriškumo įvairių sporto šakų sportininkų fizinio išsivystymo, fizinio ir funkcinio pajėgumo adaptaciniai ypatumai

    OpenAIRE

    Tubelis, Linas; Skernevičius, Juozas; Milašius, Kazys; Dadelienė, Rūta

    2006-01-01

    Kiekvienos sporto šakos varžybinė veikla yra savita, todėl ir atskirų šakų sportininkams reikia tam tikrų specialių bruožų. Dėl treniruotės ir varžybų krūvių poveikio sportininkų organizme vyksta skirtingi adaptaciniai pakitimai. Yra sporto šakų, kuriose pranašumas teikiamas didelio ūgio sportininkams, tačiau daugumoje sporto šakų puikių sportinių rezultatų gali siekti ir vidutinio arba net mažo ūgio sportininkai. Norint palyginti atskirų sporto šakų savitą poveikį sportininkų organizmui, tyr...

  10. Produkce extracelulárních hydroláz pomocí specifických druhů plísní

    OpenAIRE

    Pokrývková, Zuzana

    2015-01-01

    Táto bakalárska práca je zameraná na štúdium možností produkcie extracelulárnych hydrolytických enzýmov plesňou Penicillium lilacinum na dvoch substrátoch. Teoretická časť práce je zameraná na charakterizáciu vybraných hydrolytických enzýmov, ich vlastnosti, možnosť produkcie a ich aplikácie. V experimentálnej časti boli hydrolytické enzýmy produkované plesňou Penicillium lilacinum pri statickej kultivácií v minerálnom médiu a v médiu kde ako substrát boli použité kmene kvasiniek Sporobolomyc...

  11. Primjena zrakoplova pri gašenju požara

    OpenAIRE

    Rosavec, Roman; Španjol, Željko; Barčić, Damir; Palčić, Domagoj

    2014-01-01

    Svaki požar može se ugasiti kantom vode ako vatrogasac dovoljno brzo stigne do njega. Navedena tvrdnja upućuje na ključnu povezanost vremena reakcije na pojavu požara i utroška resursa za gašenje. U nastojanju smanjivanja broja početaka požara, uspješna služba zaštite od požara je ona koja ugasi sve požare dok još ne zahvate veliko područje. Požar ima kumulativno djelovanje, ne ponaša se i ne širi kao linearna funkcija, kako vremena tako i prostora. Požar nastaje kao posljedica zapaljivosti n...

  12. Minimally invasive veneers: current state of the art

    Directory of Open Access Journals (Sweden)

    Vanlıoğlu BA

    2014-11-01

    Full Text Available Burçin Akoğlu Vanlioğlu, Yasemin Kulak-Özkan University of Marmara, Department of Prosthetic Dentistry, Istanbul, TurkeyAbstract: Ceramic veneers are considered a conservative solution for patients requiring improvement of the shape, color, or position of their anterior teeth. Ceramic veneers have been extensively and successfully used to mask intrinsic staining, to give the appearance of straightening, and to correct minor malformations of anterior teeth without the removal of substantial amounts of sound tooth substance. The current literature was reviewed to search for the most important parameters determining the long-term success and correct application of ceramic veneers. Keywords: ceramic veneers, esthetic treatment

  13. Návrh marketingové strategie pro high-tech firmu

    OpenAIRE

    Drgonec, Igor

    2016-01-01

    Diplomová práca sa zaoberá využitím marketing a jeho pod kategórie online marketingu na vytvorenie obecného postupu alebo návodu na zavedenie a zvýšenie popularity mobilných aplikácii vyvinutých firmou Programatori s.r.o. a tým aj propagáciou firmy samotnej. V práci budú použité najmä nástroje online marketingu ako sociálne siete, a SEO optimalizácia. Tieto nástroje sa budú využívať počas celého životného cyklu aplikácie. Diploma’s thesis presents usage of marketing and subcategory online ...

  14. O arbitrabilnosti u komparativnoj arbitraži – Neke napomene

    OpenAIRE

    Sajko, Krešimir

    2010-01-01

    Objektivna arbitrabilnost određuje koji se sporovi mogu podvrći arbitražnom rješavanju, utvrđena je prinudnim materijalnim pravilima međunarodnog privatnog prava. Poredba različitih nacionalnih arbitražnih prava ukazuje na to da se ta arbitrabilnost ne odnosi samo na novčane sporove već i na nenovčane ako se o njima može zaključiti nagodbu. Na osnovi analize izabranih nacionalnih prava, sudske i arbitražne prakse te Newyorške konvencije, nameće se zaključak da arbitražni i nacionalni sudov...

  15. Internet i videoigre: uzročnici povećane agresivnosti među mladima

    OpenAIRE

    Ružić, Nataša

    2011-01-01

    Porast nasilja među mladima jedan je od najvećih problema suvremenog društva. Mnogi stručnjaci smatraju da odgovornost za to snose obitelj, škola i mediji. Digitalna generacija provodi većinu slobodnog vremena na internetu i usvaja vrijednosti koje joj nameću mediji. Prema mjerilima suvremenog društva osoba je uspješna ako je slavna, bez obzira na to kako je stekla slavu, stoga je razumljivo da mladi ljudi, željni publiciteta, na različite načine pokušavaju privući pažnju. Jedan od najbrži...

  16. Palivový článek jako alternativní zdroj el. energie

    OpenAIRE

    Kürthy, Marián

    2009-01-01

    Práca sa zaoberá technológiou palivových článkov, princípom palivových článkov a ich rozdelením. Taktiež je predstavená možnosť využitia zemného plynu ako paliva pre palivové články. V záverečnej časti práce sú uvedené dve varianty použitia palivového článku v mikrokogenerácii a technicko - ekonomické zhodnotenie mikrokogeneračnej jednotky s palivovým článkom použitej v týchto variantách.

  17. Software pro biometrické rozpoznávání duhovky lidského oka

    OpenAIRE

    Maruniak, Lukáš

    2015-01-01

    V mojej práci sa zaoberám úlohou rozpoznania ľudskej dúhovky zo snímku za pomoci použitia evolučných algoritmov. Práca sa v úvode zaoberá otázkou biometrie, jej významom a základnými pojmami, s ktorými sa v texte neskor stretávam. Následne popisujem proces rozpoznania dúhovky ako aj teóriu evolučných algoritmov. V implementačnej časti popisujem návrh a implementáciu riešenia detekcie dúhovky za pomoci evolučných algoritmov, pričom kladiem doraz na detekciu okrajov zreničky a dúhovky....

  18. Univerzální modul pro přenos naměřených dat prostřednictvím WiFi

    OpenAIRE

    Vitek, Juraj

    2014-01-01

    Táto práca opisuje problematiku prenosu dát pomocou bezdrôtového prenosu. Rozoberá teoreticky problematiku počítačových sietí, základne prenosové protokoly a ich použitie. Ďalej sú opísané štandardy a mechanizmy bezdrôtového prenosu a podrobne popísaný postup prenosu dát pomocou WiFi siete. V praktickej časti je opísaný prvotný prototyp a základné funkcie prototypu ako aj jednotlivých komponentov.

  19. Antimikrobiální aktivita šípkových čajů

    OpenAIRE

    Ďubašáková, Mária

    2016-01-01

    Cieľom tejto bakalárskej práce bolo stanovenie koncentrácie biologicky aktívnych látok ako sú polyfenoly a flavonoidy a tiež celkovú antioxidačnú kapacitu v čajových extraktoch s hlavným obsahom Rosa canina. Ďalej bolo predmetom experimentu zistenie antimikrobiálnej aktivity a účinnej koncentrácie extraktu voči vybraným baktériám. Teoretická časť je zameraná na popis prírodných antioxidačných látok a ich aktívnom účinku proti voľným radikálom, popis a rozdelenie čaju, botanický a chemický pop...

  20. Genome-wide SNP discovery in walnut with an AGSNP pipeline updated for SNP discovery in allogamous organisms

    Directory of Open Access Journals (Sweden)

    You Frank M

    2012-07-01

    Full Text Available Abstract Background A genome-wide set of single nucleotide polymorphisms (SNPs is a valuable resource in genetic research and breeding and is usually developed by re-sequencing a genome. If a genome sequence is not available, an alternative strategy must be used. We previously reported the development of a pipeline (AGSNP for genome-wide SNP discovery in coding sequences and other single-copy DNA without a complete genome sequence in self-pollinating (autogamous plants. Here we updated this pipeline for SNP discovery in outcrossing (allogamous species and demonstrated its efficacy in SNP discovery in walnut (Juglans regia L.. Results The first step in the original implementation of the AGSNP pipeline was the construction of a reference sequence and the identification of single-copy sequences in it. To identify single-copy sequences, multiple genome equivalents of short SOLiD reads of another individual were mapped to shallow genome coverage of long Sanger or Roche 454 reads making up the reference sequence. The relative depth of SOLiD reads was used to filter out repeated sequences from single-copy sequences in the reference sequence. The second step was a search for SNPs between SOLiD reads and the reference sequence. Polymorphism within the mapped SOLiD reads would have precluded SNP discovery; hence both individuals had to be homozygous. The AGSNP pipeline was updated here for using SOLiD or other type of short reads of a heterozygous individual for these two principal steps. A total of 32.6X walnut genome equivalents of SOLiD reads of vegetatively propagated walnut scion cultivar ‘Chandler’ were mapped to 48,661 ‘Chandler’ bacterial artificial chromosome (BAC end sequences (BESs produced by Sanger sequencing during the construction of a walnut physical map. A total of 22,799 putative SNPs were initially identified. A total of 6,000 Infinium II type SNPs evenly distributed along the walnut physical map were selected for the

  1. Estimation of Avalanche Hazard in the Settlement of Magurka using Elba+ Model / Posúdenie Ohrozenosti Osady Magurky Lavínami S Použitím Modelu Elba+

    Directory of Open Access Journals (Sweden)

    Bartík Martin

    2013-06-01

    Full Text Available V našej študií sme sa zamerali na moderné softvérové aplikácie umožňujúce simuláciu lavíny. Použili sme model ELBA+, pomocou ktorého sme snažili zhodnotiť ohrozenosť horského prostredia v okolí starej banskej osady Magurka (1 036 m n. m., ktorá leží v závere Ľupčianskej doline pod hlavným hrebeňom Nízkych Tatier. Do histórie sa zapísala hlavne lavína v doline Ďurková zo 14. marca 1970, ktorá dodnes patrí medzi najväčšie lavíny zaznamenané na Slovensku. S použitím archívnych údajov Strediska lavínovej prevencie v Jasnej sme sa pokúsili o jej čo najvernejšiu rekonštrukciu. Následne sme sa pokúsili o simuláciu lavíny v doline Viedenka pri použití rovnakej výšky snehu v odtrhovom pásme ako sa predpokladá pri lavíne v roku 1970. Keďže časť osady Magurka je situovaných pri ústí tejto doliny, ktorá má priamejší aj kratší priebeh ako dolina Ďurková, skúmali sme možnosť jej zásahu lavínou.

  2. High-resolution analysis of the 5'-end transcriptome using a next generation DNA sequencer.

    Directory of Open Access Journals (Sweden)

    Shin-ichi Hashimoto

    Full Text Available Massively parallel, tag-based sequencing systems, such as the SOLiD system, hold the promise of revolutionizing the study of whole genome gene expression due to the number of data points that can be generated in a simple and cost-effective manner. We describe the development of a 5'-end transcriptome workflow for the SOLiD system and demonstrate the advantages in sensitivity and dynamic range offered by this tag-based application over traditional approaches for the study of whole genome gene expression. 5'-end transcriptome analysis was used to study whole genome gene expression within a colon cancer cell line, HT-29, treated with the DNA methyltransferase inhibitor, 5-aza-2'-deoxycytidine (5Aza. More than 20 million 25-base 5'-end tags were obtained from untreated and 5Aza-treated cells and matched to sequences within the human genome. Seventy three percent of the mapped unique tags were associated with RefSeq cDNA sequences, corresponding to approximately 14,000 different protein-coding genes in this single cell type. The level of expression of these genes ranged from 0.02 to 4,704 transcripts per cell. The sensitivity of a single sequence run of the SOLiD platform was 100-1,000 fold greater than that observed from 5'end SAGE data generated from the analysis of 70,000 tags obtained by Sanger sequencing. The high-resolution 5'end gene expression profiling presented in this study will not only provide novel insight into the transcriptional machinery but should also serve as a basis for a better understanding of cell biology.

  3. High-throughput sequencing of three Lemnoideae (duckweeds chloroplast genomes from total DNA.

    Directory of Open Access Journals (Sweden)

    Wenqin Wang

    Full Text Available BACKGROUND: Chloroplast genomes provide a wealth of information for evolutionary and population genetic studies. Chloroplasts play a particularly important role in the adaption for aquatic plants because they float on water and their major surface is exposed continuously to sunlight. The subfamily of Lemnoideae represents such a collection of aquatic species that because of photosynthesis represents one of the fastest growing plant species on earth. METHODS: We sequenced the chloroplast genomes from three different genera of Lemnoideae, Spirodela polyrhiza, Wolffiella lingulata and Wolffia australiana by high-throughput DNA sequencing of genomic DNA using the SOLiD platform. Unfractionated total DNA contains high copies of plastid DNA so that sequences from the nucleus and mitochondria can easily be filtered computationally. Remaining sequence reads were assembled into contiguous sequences (contigs using SOLiD software tools. Contigs were mapped to a reference genome of Lemna minor and gaps, selected by PCR, were sequenced on the ABI3730xl platform. CONCLUSIONS: This combinatorial approach yielded whole genomic contiguous sequences in a cost-effective manner. Over 1,000-time coverage of chloroplast from total DNA were reached by the SOLiD platform in a single spot on a quadrant slide without purification. Comparative analysis indicated that the chloroplast genome was conserved in gene number and organization with respect to the reference genome of L. minor. However, higher nucleotide substitution, abundant deletions and insertions occurred in non-coding regions of these genomes, indicating a greater genomic dynamics than expected from the comparison of other related species in the Pooideae. Noticeably, there was no transition bias over transversion in Lemnoideae. The data should have immediate applications in evolutionary biology and plant taxonomy with increased resolution and statistical power.

  4. The Distrbution of Various Mercury Species in Soil

    Institute of Scientific and Technical Information of China (English)

    冯新斌; 陈业材; 等

    1997-01-01

    According to the mercury species with different solubilities,the analytical procedure involving sequential chemical extraction has been applied to partitioning the mercury species in soils into seven fractions.Soil samples collected from five localities in different areas (the high-mercury ares.the man-made mercury-polluted area and the reference area)were an alyzed for the seven mercury species.It is found that high mercury contents of soils can be attributed to both man-made pollution and geological processes,but the two kinds of solis show obvious differences in the distribution of their mercury species.

  5. Sezonska variabilnost električne upornosti kambijeve cone izbranih drevesnih vrst v urbanem okolju

    OpenAIRE

    Jager, Dušan

    2014-01-01

    Preučevali smo vitalnost mestnih dreves z merjenjem električne upornosti živihtkiv (CER) v drevesu z instrumentom, imenovanim Tree tester. Meritve smo opravljali enkrat mesečno med rastno sezono 2005, od meseca maja do meseca oktobra. Drevesa smo ocenili tudi vizualno in jih razvrstili v skupine po prizadetosti krošnje zaradi neustrezne nege (obglavljena drevesa) in prizadetosti krošnje zaradi posipnih soli ter zdrava oz. referenčna drevesa. Drevesa smo razvrstili tudi v razširjene debelinske...

  6. Seasonal cycle of hydrography in the Bab el Mandab region, southern Red Sea

    Digital Repository Service at National Institute of Oceanography (India)

    Saafani, M.A.A.; Shenoi, S.S.C.

    circulation where a fresh inflow prevails over a hypersaline outflow. During this period, the upper layer ( 80 m deep) expe- riences an inflow while the deeper layer (below 80 m) experiences an outflow (Maillard and Soli- man 1986; Murray and Johns 1997... winds. It is possible that the pocket of warm fresh water found on the western side ( g- ure 5d and e) near the sill is a manifestation of downwelling. The model studies of So anos and Johns (2003) also indicate wind induced upwelling along the eastern...

  7. デカルトの物心二元論再考

    OpenAIRE

    立花, 希一; TACHIBANA, Kiichi

    2008-01-01

    Descartes (lS96-16SOlis the first figure who proposed body-mind dualism based on his own rational arguments, not based on any allegorical or philosophical interpretations of the Bible, which was primarily acceptedas the absolute authority. In this paper I critically examine Descartes' arguments, and point out his wrong strategy. that is, we ought to exclude as false all these things of which we may doubt. I claim that Descartes' philosophical opinion is not the sole absolutely certain truth, ...

  8. Preživljavanje bakterije Listeria monocytogenes u Ayranu, tradicionalnom turskom fermentiranom napitku

    OpenAIRE

    Akkaya, Levent; Kara, Recep; Muduroglu, Raziye; Sagdic, Osman

    2015-01-01

    Ayran je tradicionalni fermentirani mliječni proizvod koji se dobiva miješanjem mlijeka ili jogurta, te vode i soli. U ovom je istraživanju ispitivano preživljavanje soja Listeria monocytogenes 1/2b u uzorcima Ayrana. U tu su svrhu proizvedeni uzorci Ayrana iz jogurta (skupina A i B) ili izravno iz mlijeka (skupina C i D) te kontaminirani dodatkom 1 % kulture soja L. monocytogenes 1/2b u koncentraciji 7 (uzorci A1, B1, C1 i D1) ili 5 (uzorci A2, B2, C2 i D2) log cfu/mL. Time je proizvedeno uk...

  9. Territorialisation et circulation des échanges sociaux dans deux sites de la banlieue parisienne

    OpenAIRE

    Benveniste, Annie

    2000-01-01

    L'article analyse la construction de nouvelles territorialités en liaison avec le développement des mouvements ethno-religieux. Deux sites ont été choisis qui avaient fait l'objet d'une recherche sur la ségrégation résidentielle : Sarcelles et Garges-les-Gonesse. L'analyse porte d'abord sur les transformations des espaces, leur appropriation et la recombinaison du public et du privé qui permet de s'émanciper des contraintes de la relégation. Elle porte ensuite sur les nouvelles formes de soli...

  10. Coverage bias and sensitivity of variant calling for four whole-genome sequencing technologies.

    Directory of Open Access Journals (Sweden)

    Nora Rieber

    Full Text Available The emergence of high-throughput, next-generation sequencing technologies has dramatically altered the way we assess genomes in population genetics and in cancer genomics. Currently, there are four commonly used whole-genome sequencing platforms on the market: Illumina's HiSeq2000, Life Technologies' SOLiD 4 and its completely redesigned 5500xl SOLiD, and Complete Genomics' technology. A number of earlier studies have compared a subset of those sequencing platforms or compared those platforms with Sanger sequencing, which is prohibitively expensive for whole genome studies. Here we present a detailed comparison of the performance of all currently available whole genome sequencing platforms, especially regarding their ability to call SNVs and to evenly cover the genome and specific genomic regions. Unlike earlier studies, we base our comparison on four different samples, allowing us to assess the between-sample variation of the platforms. We find a pronounced GC bias in GC-rich regions for Life Technologies' platforms, with Complete Genomics performing best here, while we see the least bias in GC-poor regions for HiSeq2000 and 5500xl. HiSeq2000 gives the most uniform coverage and displays the least sample-to-sample variation. In contrast, Complete Genomics exhibits by far the smallest fraction of bases not covered, while the SOLiD platforms reveal remarkable shortcomings, especially in covering CpG islands. When comparing the performance of the four platforms for calling SNPs, HiSeq2000 and Complete Genomics achieve the highest sensitivity, while the SOLiD platforms show the lowest false positive rate. Finally, we find that integrating sequencing data from different platforms offers the potential to combine the strengths of different technologies. In summary, our results detail the strengths and weaknesses of all four whole-genome sequencing platforms. It indicates application areas that call for a specific sequencing platform and disallow other

  11. Návrh jednotného BI řešení pro sadu nabízených ERP skupiny Solitea

    OpenAIRE

    Sládková, Lenka

    2016-01-01

    Subject of honor thesis is self-service business intelligence. Its goal is to prove that it is pos-sible to create a unified industry-specific dashboard above set of different ERP systems. First part familiarizes readers with the main theme of the paper, business intelligence, and selected tool for dashboard creation, Microsoft Power BI. The next part characterizes Soli-tea holding of which project is used and described later in this paper. There is also a place for analysis of all ERP system...

  12. Biological Treatment of Wastewater by Sequencing Batch Reactors

    OpenAIRE

    Tsvetko Prokopov; Dasha Mihaylova; Nikolay Mihalkov

    2014-01-01

    In the present paper the operation of wastewater treatment plant (WWTP) in the town of Hisarya which includes a biological stage with aeration basins of cyclic type (SBR-method) was studied. The values of the standard indicators of input and output water from the wastewater treatment plant were evaluated. Moreover, the reached effects due to the biological treatment of the wastewater in terms of the COD (95.7%), BOD5 (96.6%), total nitrogen (81.3%), total phosphorus (53.7%) and suspended soli...

  13. Genomic research for important pathogenic bacteria in China

    Institute of Scientific and Technical Information of China (English)

    YANG RuiFu; GUO XiaoKui; YANG Jian; JIANG YongQiang; PANG Bo; CHEN Chen; YAO YuFeng; QIN JinHong; LI QingTian

    2009-01-01

    Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of point. We can thus closely look at the way in which a pathogen is evolved, and these data has been applied to molecular epidemiology and microbial forensics, and screening of novel diagnostic, vaccine and drug targets. The newly developed high-throughput low-cost sequencing technologies, such as 454, Solexa and SOLiD, will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously, such as the metagenomics of human gastric-intestinal tract, for better and comprehensive understanding of human health and disease.

  14. HMI Synoptic Maps Produced by NSO/NISP

    CERN Document Server

    Hughes, Anna L H; Marble, Andrew R; Oien, Niles A; Petrie, Gordon; Pevtsov, Alexei A

    2016-01-01

    Recently, the National Solar Observatory (NSO) Solar-atmosphere Pipeline Working Group has undertaken the production of synoptic maps from Helioseismic and Magnetic Imager (HMI) magnetograms. A set of maps has been processed spanning the data available for 2010-2015 using twice daily images (taken at UT midnight and noon) and running them through the same algorithms used to produce SOLIS/VSM 6302l mean-magnetic and spatial-variance maps. The contents of this document provide an overview of what these maps look like, and the processing steps used to generate them from the original HMI input data.

  15. Akreditacija analitičkih laboratorija i provjera vrsnosti rada: iskustva Tajlanda u svezi s analizom pesticida

    OpenAIRE

    Vongbuddhapitak, Amara; Pavittranon, Sumol; Tayaputch, Nuansri; Issaravanich, Somchai; Sinhaseni, Palarp

    1999-01-01

    Ekonomski rast Tajlanda usko je vezan s proizvodnjom oslonjenom na poljoprivredu. Stoga ova zemlja pridaje veliku važnost međunarodnim normama koje usklađuju međunarodni promet hrane i štite zdravlje potrošača od kemikalija bilo u obliku aditiva ili kao onečišćivala (engl. contaminant). U Tajlandu su u širokoj primjeni antikolinesterazni i piretroidni pesticidi, derivati kumarina, soli bi-piridinija te donekle organoklorirani spojevi kao što je DDT koji se rabi za kontrolu malarije. Testiranj...

  16. Música y matemáticas

    OpenAIRE

    Blázquez Lozano, Rosa Marina

    2014-01-01

    Durante muchos siglos se ha considerado que las matemáticas y la música tienen cierta similitud y comúnmente se dice que tienen al menos cierta relación. Ambas tienen algo de mágico, comenta Tiburcio Solis (2002), son tan abstractas que parecen pertenecer a otro mundo y sin embargo tienen gran poder en este mundo, la música afecta a la escucha y las matemáticas tienen múltiples aplicaciones prácticas. Una parte de las matemáticas estudia los números, sus patrones y formas y estos elementos...

  17. A detailed analysis of next generation sequencing reads of microRNA expression in Barrett’s Esophagus: absolute versus relative quantification

    OpenAIRE

    Lee, In-Hee; Hong, Xiaoman; Mathur, Sharad C; Sharma, Mukut; Rastogi, Amit; Sharma, Prateek; Christenson, Lane K.; Bansal, Ajay

    2014-01-01

    Background Next generation sequencing (NGS) is a state of the art technology for microRNA (miRNA) analysis. The quantitative interpretation of the primary output of NGS i.e. the read counts for a miRNA sequence that can vary by several orders of magnitude (1 to 107) remains incompletely understood. Findings NGS (SOLiD 3 technology) was performed on biopsies from 6 Barrett’s esophagus (BE) and 5 Gastroesophageal Reflux Disease (GERD) patients. Read sequences were aligned to miRBase 18.0. Diffe...

  18. Effect of prefeeding lipid on food intake and satiety in man.

    OpenAIRE

    Sepple, C P; Read, N W

    1990-01-01

    Experiments were carried out in normal volunteers to investigate whether preingestion of lipid reduces food intake. In the first set of experiments, 300 ml beef consomme soup with or without 60 g margarine was fed to each of six volunteers, followed 20 minutes later by either a low fat solid meal or a preselected appetising meal. Subjects were allowed to eat as much of the meal as they wished. Preingestion of the high fat soup had no significant effect on the consumption of either of the soli...

  19. Comparison of MicroRNA Deep Sequencing of Matched Formalin-Fixed Paraffin-Embedded and Fresh Frozen Cancer Tissues

    OpenAIRE

    Wei Meng; McElroy, Joseph P.; Stefano Volinia; Jeff Palatini; Sarah Warner; Ayers, Leona W; Kamalakannan Palanichamy; Arnab Chakravarti; Tim Lautenschlaeger

    2013-01-01

    MicroRNAs regulate several aspects of tumorigenesis and cancer progression. Most cancer tissues are archived formalin-fixed and paraffin-embedded (FFPE). While microRNAs are a more stable form of RNA thought to withstand FFPE-processing and degradation there is only limited evidence for the latter assumption. We examined whether microRNA profiling can be successfully conducted on FFPE cancer tissues using SOLiD ligation based sequencing. Tissue storage times (2-9 years) appeared to not affect...

  20. Intoxicaciones agudas en Santiago de Compostela,en un período de cuatro años

    OpenAIRE

    Fernández, P.; M. Ortega; A.M. Bermejo; M.J. Tabernero; M. López Rivadulla; ME Concheiro

    2003-01-01

    El objetivo de este trabajo fue evaluar las caracterís-ticas de un grupo de pacientes intoxicados en el área sanitaria deSantiago de Compostela. Para ello, se estudiaron 1192 intoxica-ciones agudas atendidas en el Servicio de Urgencias y Unidadde Cuidados Intensivos del Hospital Clínico Universitario deesta ciudad, desde el 1 de enero de 1993 al 31 de diciembre de1996. Se han considerado solo aquellos casos en los que se soli-citó un análisis toxicológico a este Instituto de Medicina Legal,de...

  1. Derechos Humanos y competencia exclusiva del Estado en materia de nacionalidad (La sentencia de la Corte Interamericana de Derechos Humanos de 28 de agosto de 2104: Caso Personas dominicanas y haitianas expulsadas Vs. Rep??blica Dominicana)

    OpenAIRE

    S??nchez Lorenzo, Sixto A.

    2015-01-01

    La sentencia de la CIDH de 28 de agosto de 2014 obliga a la Rep??blica Dominicana a modificar su Constituci??n para conceder la nacionalidad por ius soli a los hijos de padres extranjeros que residan irregularmente en territorio dominicano. Sobre la base de los derechos humanos a la personalidad jur??dica, a la no discriminaci??n y a la nacionalidad, la CIDH determina un recorte sin precedentes a la competencia exclusiva del Estado en materia de nacionalidad. La presente nota contiene un an??...

  2. Značenje halofilnih bakterija u preradi mesa i ribe

    OpenAIRE

    Mioković, Branimir; Zdolec, Nevijo

    2004-01-01

    Soljenje, kao najstariji oblik konzerviranja hrane na razvoj mikroorganizama djeluje na način da sol oduzima vodu, a kao posljedica toga je sistiranje rasta, sporuliranje ili ugibanje bakterija. Bakterije koje mogu živjeti u medijima s povećanom količinom soli nazivaju se halofilne bakterije. U okviru ovog rada prikazali smo vrste i značenje halofilnih bakterija u preradi namirnica životinjskog podrijetla. Vrsta i broj bakterija u soljenim namirnicama različita je, a ovisi o mnogim čimben...

  3. LE STAZIONI DI APPRENDIMENTO: ESEMPI DIDATTICI PER UN APPROCCIO DI TIPO GLOBALE ALL’INSEGNAMENTO DELLA LINGUA ITALIANA A STRANIERI

    OpenAIRE

    Antonietta Gobbis; Marco Paoli Legler

    2015-01-01

    Il metodo, che si rifà alle teorie pedagogiche di Maria Montessori (1870-1952), è un esempio di lezione “aperta” fondato su pratiche che attivano la sfera emozionale, sensoriale e relazionale oltre a quella logico-razionale della mente. Nel processo di apprendimento di conoscenze acquisite non solo attraverso l’ascolto delle parole dell’insegnante, ma anche attraverso esperienze scelte autonomamente e consapevolmente da soli e in gruppo, l’ambiente delle stazioni didattiche offre tutto il mat...

  4. Semi-solid Processing of Al-7Graghite Composite

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Al-7graphite composite was processed using Al-7graphite mush y prepared by electromagnetic-mechanical stirring method, and the infl uence of solid fraction on the distributing of graphite particles in i ngot was studied. The results shows that the relationship between soli d fraction and stirring temperature of mushy is: =591.50.897 (where i s the solid fraction, is the stirring temperature). For Al-7graphite composite, with the increasing of solid fraction, the aggregation exte nt of graphite particles reduced gradually, and when solid fraction wa s larger than 30%, graphite particles could distribute evenly in ingot .

  5. FadE: whole genome methylation analysis for multiple sequencing platforms.

    Science.gov (United States)

    Souaiaia, Tade; Zhang, Zheng; Chen, Ting

    2013-01-01

    DNA methylation plays a central role in genomic regulation and disease. Sodium bisulfite treatment (SBT) causes unmethylated cytosines to be sequenced as thymine, which allows methylation levels to reflected in the number of 'C'-'C' alignments covering reference cytosines. Di-base color reads produced by lifetech's SOLiD sequencer provide unreliable results when translated to bases because single sequencing errors effect the downstream sequence. We describe FadE, an algorithm to accurately determine genome-wide methylation rates directly in color or nucleotide space. FadE uses SBT unmethylated and untreated data to determine background error rates and incorporate them into a model which uses Newton-Raphson optimization to estimate the methylation rate and provide a credible interval describing its distribution at every reference cytosine. We sequenced two slides of human fibroblast cell-line bisulfite-converted fragment library with the SOLiD sequencer to investigate genome-wide methylation levels. FadE reported widespread differences in methylation levels across CpG islands and a large number of differentially methylated regions adjacent to genes which compares favorably to the results of an investigation on the same cell-line using nucleotide-space reads at higher coverage levels, suggesting that FadE is an accurate method to estimate genome-wide methylation with color or nucleotide reads. http://code.google.com/p/fade/.

  6. Cyber threat impact assessment and analysis for space vehicle architectures

    Science.gov (United States)

    McGraw, Robert M.; Fowler, Mark J.; Umphress, David; MacDonald, Richard A.

    2014-06-01

    This paper covers research into an assessment of potential impacts and techniques to detect and mitigate cyber attacks that affect the networks and control systems of space vehicles. Such systems, if subverted by malicious insiders, external hackers and/or supply chain threats, can be controlled in a manner to cause physical damage to the space platforms. Similar attacks on Earth-borne cyber physical systems include the Shamoon, Duqu, Flame and Stuxnet exploits. These have been used to bring down foreign power generation and refining systems. This paper discusses the potential impacts of similar cyber attacks on space-based platforms through the use of simulation models, including custom models developed in Python using SimPy and commercial SATCOM analysis tools, as an example STK/SOLIS. The paper discusses the architecture and fidelity of the simulation model that has been developed for performing the impact assessment. The paper walks through the application of an attack vector at the subsystem level and how it affects the control and orientation of the space vehicle. SimPy is used to model and extract raw impact data at the bus level, while STK/SOLIS is used to extract raw impact data at the subsystem level and to visually display the effect on the physical plant of the space vehicle.

  7. Full-Disk Chromospheric Vector Magnetograms with Ca II 854.2 nm line: Some Promising Applications

    Science.gov (United States)

    Gosain, Sanjay; Harvey, J. W.; Harker, Brian; Pillet, V. M.; Pevtsov, Alexei A.; Marble, Andrew R.; Bertello, Luca; + SOLIS-Team

    2016-05-01

    Over the last decade, the focus of solar magnetometry has shifted outward from the photosphere to the chromospheric layers. The reasons for this are many. With regards to instrumentation faster detectors with more sensitivity have become available, as have fast electro-optic modulators. Also, there are several potential benefits of observing vector fields in the chromospheric layer as the magnetic field is more force-free in this layer as compared to the photosphere. Coronal force-free field extrapolations are more reliable using chromospheric fields as the lower boundary condition and free magnetic energy is readily computed using the magnetic virial theorem. Recently, a full Stokes polarimeter for the chromospheric Ca II 854.2 nm spectral line was developed and installed in the Vector Spectromagnetograph (VSM) instrument on the Synoptic Optical Long-term Investigations of the Sun (SOLIS) telescope. We present details of this new polarimeter, full disk spectropolarimetric observations and vector magnetograms of the chromosphere, and examples of some promising applications (e.g., maps of normal component of electric current density in the chromosphere, free magnetic energy estimated using virial theorem, and non-potentiality parameter magnetic shear angle).This work utilizes SOLIS data obtained by the NSO Integrated Synoptic Program (NISP), managed by the National Solar Observatory, which is operated by the Association of Universities for Research in Astronomy (AURA), Inc. under a cooperative agreement with the National Science Foundation

  8. Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium.

    Science.gov (United States)

    Simon, Anaele; Bindschedler, Saskia; Job, Daniel; Wick, Lukas Y; Filippidou, Sevasti; Kooli, Wafa M; Verrecchia, Eric P; Junier, Pilar

    2015-11-01

    Fungi and bacteria form various associations that are central to numerous environmental processes. In the so-called fungal highway, bacteria disperse along fungal mycelium. We developed a novel tool for the in situ isolation of bacteria moving along fungal hyphae as well as for the recovery of fungi potentially involved in dispersal, both of which are attracted towards a target culture medium. We present the validation and the results of the first in situ test. Couples of fungi and bacteria were isolated from soil. Amongst the enriched organisms, we identified several species of fast-growing fungi (Fusarium sp. and Chaetomium sp.), as well as various potentially associated bacterial groups, including Variovorax soli, Olivibacter soli, Acinetobacter calcoaceticus, and several species of the genera Stenotrophomonas, Achromobacter and Ochrobactrum. Migration of bacteria along fungal hyphae across a discontinuous medium was confirmed in most of the cases. Although the majority of the bacteria for which migration was confirmed were also positive for flagellar motility, not all motile bacteria dispersed using their potential fungal partner. In addition, the importance of hydrophobicity of the fungal mycelial surface was confirmed. Future applications of the columns include targeting different types of microorganisms and their interactions, either by enrichment or by state of the art molecular biological methods.

  9. Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium.

    Science.gov (United States)

    Simon, Anaele; Bindschedler, Saskia; Job, Daniel; Wick, Lukas Y; Filippidou, Sevasti; Kooli, Wafa M; Verrecchia, Eric P; Junier, Pilar

    2015-11-01

    Fungi and bacteria form various associations that are central to numerous environmental processes. In the so-called fungal highway, bacteria disperse along fungal mycelium. We developed a novel tool for the in situ isolation of bacteria moving along fungal hyphae as well as for the recovery of fungi potentially involved in dispersal, both of which are attracted towards a target culture medium. We present the validation and the results of the first in situ test. Couples of fungi and bacteria were isolated from soil. Amongst the enriched organisms, we identified several species of fast-growing fungi (Fusarium sp. and Chaetomium sp.), as well as various potentially associated bacterial groups, including Variovorax soli, Olivibacter soli, Acinetobacter calcoaceticus, and several species of the genera Stenotrophomonas, Achromobacter and Ochrobactrum. Migration of bacteria along fungal hyphae across a discontinuous medium was confirmed in most of the cases. Although the majority of the bacteria for which migration was confirmed were also positive for flagellar motility, not all motile bacteria dispersed using their potential fungal partner. In addition, the importance of hydrophobicity of the fungal mycelial surface was confirmed. Future applications of the columns include targeting different types of microorganisms and their interactions, either by enrichment or by state of the art molecular biological methods. PMID:26432804

  10. High-Throughput Sequencing of MicroRNAs in Adenovirus Type 3 Infected Human Laryngeal Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Yuhua Qi

    2010-01-01

    Full Text Available Adenovirus infection can cause various illnesses depending on the infecting serotype, such as gastroenteritis, conjunctivitis, cystitis, and rash illness, but the infection mechanism is still unknown. MicroRNAs (miRNA have been reported to play essential roles in cell proliferation, cell differentiation, and pathogenesis of human diseases including viral infections. We analyzed the miRNA expression profiles from adenovirus type 3 (AD3 infected Human laryngeal epithelial (Hep2 cells using a SOLiD deep sequencing. 492 precursor miRNAs were identified in the AD3 infected Hep2 cells, and 540 precursor miRNAs were identified in the control. A total of 44 miRNAs demonstrated high expression and 36 miRNAs showed lower expression in the AD3 infected cells than control. The biogenesis of miRNAs has been analyzed, and some of the SOLiD results were confirmed by Quantitative PCR analysis. The present studies may provide a useful clue for the biological function research into AD3 infection.

  11. The role of measured data in a free natural gas market

    International Nuclear Information System (INIS)

    As from 2002 medium-scale customers connected to the distribution network of the regional natural gas distribution companies will be able to purchase their gas from third parties. In order to calculate the energy (gas) transmission, distribution and supply costs, it is necessary to measure and record the offtakes of all the customers with freedom of choice on an hourly basis. The Dutch natural gas trading company Gasunie usually carries out these measurements for the present 350 medium-scale customers, but for the 14,000 customers by 2002 a different solution probably must be chosen. This is because most offtakes do not have any provision for measuring consumption on an hourly basis. Following the electricity market, offtake profiles will be prepared for most customers. If the parties cannot reach agreement on those profiles, expensive measuring equipment has to be installed on site. The Standard Online Information Server (Solis) is used in a project to transfer the data measured online from a remote customer to the electricity supplier. Solis will also be used for transferring gas data. The Dutch natural gas research institute Gastec has carried out a definition study into the use of gas consumption profiles for assessing the gas balance. Gastec and KEMA (research institute for the Dutch electric power companies) will jointly further develop this gas consumption model for both the gas and electricity market

  12. THE INFLUENCE OF FLOOR QUALITY ON DAIRY COW BEHAVIOUR AND THEIR BODY CLEANLINESS VPLYV KVALITY PODLAHY NA SPRÁVANIE DOJNÍC A ICH ČISTOTU POVRCHU TELA

    Directory of Open Access Journals (Sweden)

    Emília KUŠNÍROVÁ

    2012-03-01

    Full Text Available The aim of this study was to evaluate the influence of floor quality on the behaviour and cleanliness of the diary cow‘s body. The analysis results show that cows housed in the section with the separated manure solids spent more time lying (41.47% and also laid less in the corridor manure (0.91% than the cows in the section with separated manure solids and the rubber mats where they were laying generally less (35.71%, they spent more time lying in the corridor manure (1.55% and in terms of preference of lying area the cows clearly preferred the separated manure solids (25.8%. Based on observations we can say that cows housed in sections of slurry solid phase were cleaner (average 6.13 points than cows in the section with the rubber and a separated manure (averagely 7.28 points. Nowadays the free housing systems with different types of litter and boxes are frequently used, while the proper littering influences the lying preferences of an animal. In practice, for the dairy cows the deepened lying boxes littered with natural materials such as sawdust, shavings, straw, dried manure and sand boxes or elevated boxes covered with a synthetic material, mattresses or mats are used.Cieľom práce bolo zhodnotiť vplyv kvality podlahy na správanie a čistotu povrchu tela dojníc. Z analýzy výsledkov vyplýva, že dojnice ustajnené v sekcii so separovaným kalom hnojovice venovali ležaniu väčšiu časť (41,47% a ležali aj menej v hnojnej chodbe (0,91% ako dojnice v sekcii so separovaným kalom hnojovice aj gumenými matracmi, kde dojnice celkovo menej ležali (35,71%, viac času ležali v hnojnej chodbe (1,55% a z hľadiska uprednostňovania ležoviska ležali jednoznačne viac na separovanom kale hnojovice (25,8%. Na základe pozorovaní možno povedať, že dojnice ustajnené v sekciách so separovaným kalom hnojovice boli čistejšie (priemerne 6,13 bodov ako dojnice v sekcii so separovaným kalom hnojovice aj gumou (priemerne 7,28 bodov. V s

  13. Similar foliage area but contrasting foliage biomass between young beech and spruce stands / Porovnateľná plocha avšak kontrastná biomasa asimilačných orgánov medzi mladými porastmi buka a smreka

    Directory of Open Access Journals (Sweden)

    Konôpka Bohdan

    2014-12-01

    Full Text Available Výskum sa zameral na mladé porasty buka lesného (Fagus sylvatica a smreka obyčajného (Picea abies rovnakého veku (12 rokov, veľmi podobných dimenzií stromov, rastúcich na totožnom stanovišti. Na základe odberu vzorníkov celých stromov (všetky časti okrem jemných koreňov sme skonštruovali alometrické vzťahy pre stromové komponenty. Ako nezávislá premenná sa použila hrúbka na báze kmeňa (d0. Modely vyjadrili nielen biomasu konárov, kmeňa, hrubých koreňov a asimilačných orgánov, ale aj plochu asimilačných orgánov a špecifickú listovú plochu (specific leaf area; SLA. Zistili sme, že základné morfologické vlastnosti asimilačných orgánov varírovali pri obidvoch drevinách pozdĺž vertikálneho profilu koruny. V prípade smreka sa zistili odlišné hodnoty plochy ihlíc a SLA medzi jednotlivými ročníkmi ihlíc. Na úrovni stromu mali buky oveľa viac biomasy drevných častí ako smreky, opačná situácia bola pri asimilačných orgánoch. Preto hodnoty podielu medzi biomasou asimilačných orgánov a celkovou biomasou stromu, ako aj pomeru medzi plochou asimilačných orgánov a celkovou biomasou stromu boli výrazne vyššie pri smreku než buku. Na úrovni porastu mala smrečina vyššie hodnoty indexu listovej plochy, t. j. LAI (18,64 m2.m−2 v porovnaní s bučinou (12,77 m2.m−2. Kým biomasa asimilačných orgánov bola 4,6-krát väčšia v smrekovom než v bukovom poraste, biomasa drevných časti bola porovnateľná v obidvoch porastoch. Tieto kontrasty naznačujú výrazne odlišnú rastovú stratégiu, resp. alokáciu biomasy medzi bučinami a smrečinami v mladých štádiách

  14. Primjena spektrometrijskih metoda u analizi metalurških uzoraka

    Directory of Open Access Journals (Sweden)

    Anita Štrkalj

    2016-07-01

    Full Text Available Instrumentalne metode analize nezamjenjive su u znanstvenim istraživanjima, a sve više i u praksi gdje se zahtijeva što brže dobivanje rezultata, kao i što veća točnost. U metalurškoj praksi već se dulji niz godina primjenjuju spektrometrijske metode analize i to najčešće optička emisijska spektrometrija s izravnim očitanjem. Međutim koncentracija pojedinih legirnih elemenata u suvremenim metalnim materijalima vrlo je niska, često niža od granice detekcije optičkih emisijskih spektrometara s izravnim očitanjem. U ovom radu određivana je koncentracija bora u nodularnom lijevu primjenom optičke emisijske spektrometrije s izravnim očitanjem (OES, optičke emisijske spektrometrije s induktivno spregnutom plazmom (ICP-OES te atomske apsorpcijske spektrometrije s grafitnom tehnikom (AAS-GF. Dobiveni rezultati pokazali su da se optička emisijska spektrometrija s izravnim očitanjem i optička emisijska spektrometrija s induktivno spregnutom plazmom mogu primijeniti za određivanje bora u ispitivanim uzorcima (tablica 1. Ako se zahtijeva vrlo točna analiza, a posebno ako se radi o vrlo niskim koncentracijama, optička emisijska spektrometrija s induktivno spregnutom plazmom pogodnija je metoda, uz uvjet da su uzorci pravilno pripremljeni. Atomska apsorpcijska spektrometrija s grafitnom tehnikom nije pogodna za određivanje bora u uzorcima ovog tipa. Tijekom provođenja analize dodatna pažnja usmjerena je na eliminaciju negativnog utjecaja grafitne kivete na rezultat analize. Osim toga, u radu je praćen utjecaj načina otapanja uzoraka na rezultate analize bora spomenutim metodama. Rezultati su pokazali da pažljiva priprema uzoraka u zatvorenom sustavu pridonosi točnijem rezultatu analize.

  15. CLINICAL COMPARISION OF THE TREATMENT OF CERVICAL PROLAPSE OF INTERVERTEBRAL DISC BY USING CAGE OR TITANIUM PLATE

    Institute of Scientific and Technical Information of China (English)

    Yang Pei; Wang Kunzheng; Gong Fuliang; Wang Chunsheng; Shi Zhibin

    2006-01-01

    Objective To compare the clinical effects of treating cervical prolapse of intervertebral disc by using Solis cage, titanium cage and autogenous iliac crest graft (AICG) combined with titanium plate. Methods 64 cases of cervical prolapse of intervertebral disc were analyzed retrospectively. All the patients were followed up for about one year. Group A is composed of 20 patients (30 intervertebral spaces) treated with microdiscectomy and Solis cage fusion;group B is composed of 21 patients(22 intervertebral spaces) treated with titanium cage and group C is involved of 23patients(28 intervertebral spaces) which were dealt with AICG combined with titanium plate. The differences in the to-tal X ray exposure time, time working on iliac bone, incidence of complications, fusion rate, incidence of JOA score recovery rate more than 50 % and rehabilitation time of the three groups are analyzed. Results All indexes from group A and B were more satisfactory than those from group C (P<0. 05). The statistic analysis results of the incidence of complications of cervical part, rehabilitation time in group A are 5.0± 1.8, 5.1 ± 1.2; and that is 14.3±2.6, 7.5 ± 1.6and 26.1±6.2, 8.6±2.3 in group B and group C respectively. There is significant difference between group A and group B on the incidence of complications of cervical part and rehabilitation time (P<0.05). Results of the incidence of JOA score recovery rate > 50 % in group A(95.8 ± 3.2) was more satisfactory than that in group B(93.6 ± 2.9),while there was no statistical difference between group A and group B (P>0.05). Conclusion The Solis cage is better in treating the cervical prolapse of intervertebral disc than other ways. Also, it makes the operation easier, rehabilitation time shorter, and it causes fewer complications.

  16. Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos

    International Nuclear Information System (INIS)

    Although many drugs and environmental chemicals are teratogenic, the mechanisms by which most toxicants disrupt embryonic development are not well understood. MicroRNAs, single-stranded RNA molecules of ∼ 22 nt that regulate protein expression by inhibiting mRNA translation and promoting mRNA sequestration or degradation, are important regulators of a variety of cellular processes including embryonic development and cellular differentiation. Recent studies have demonstrated that exposure to xenobiotics can alter microRNA expression and contribute to the mechanisms by which environmental chemicals disrupt embryonic development. In this study we tested the hypothesis that developmental exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a well-known teratogen, alters microRNA expression during zebrafish development. We exposed zebrafish embryos to DMSO (0.1%) or TCDD (5 nM) for 1 h at 30 hours post fertilization (hpf) and measured microRNA expression using several methods at 36 and 60 hpf. TCDD caused strong induction of CYP1A at 36 hpf (62-fold) and 60 hpf (135-fold) as determined by real-time RT-PCR, verifying the effectiveness of the exposure. MicroRNA expression profiles were determined using microarrays (Agilent and Exiqon), next-generation sequencing (SOLiD), and real-time RT-PCR. The two microarray platforms yielded results that were similar but not identical; both showed significant changes in expression of miR-451, 23a, 23b, 24 and 27e at 60 hpf. Multiple analyses were performed on the SOLiD sequences yielding a total of 16 microRNAs as differentially expressed by TCDD in zebrafish embryos. However, miR-27e was the only microRNA to be identified as differentially expressed by all three methods (both microarrays, SOLiD sequencing, and real-time RT-PCR). These results suggest that TCDD exposure causes modest changes in expression of microRNAs, including some (miR-451, 23a, 23b, 24 and 27e) that are critical for hematopoiesis and cardiovascular

  17. Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos

    Energy Technology Data Exchange (ETDEWEB)

    Jenny, Matthew J. [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487 (United States); Aluru, Neelakanteswar [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Hahn, Mark E., E-mail: mhahn@whoi.edu [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States)

    2012-10-15

    Although many drugs and environmental chemicals are teratogenic, the mechanisms by which most toxicants disrupt embryonic development are not well understood. MicroRNAs, single-stranded RNA molecules of ∼ 22 nt that regulate protein expression by inhibiting mRNA translation and promoting mRNA sequestration or degradation, are important regulators of a variety of cellular processes including embryonic development and cellular differentiation. Recent studies have demonstrated that exposure to xenobiotics can alter microRNA expression and contribute to the mechanisms by which environmental chemicals disrupt embryonic development. In this study we tested the hypothesis that developmental exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a well-known teratogen, alters microRNA expression during zebrafish development. We exposed zebrafish embryos to DMSO (0.1%) or TCDD (5 nM) for 1 h at 30 hours post fertilization (hpf) and measured microRNA expression using several methods at 36 and 60 hpf. TCDD caused strong induction of CYP1A at 36 hpf (62-fold) and 60 hpf (135-fold) as determined by real-time RT-PCR, verifying the effectiveness of the exposure. MicroRNA expression profiles were determined using microarrays (Agilent and Exiqon), next-generation sequencing (SOLiD), and real-time RT-PCR. The two microarray platforms yielded results that were similar but not identical; both showed significant changes in expression of miR-451, 23a, 23b, 24 and 27e at 60 hpf. Multiple analyses were performed on the SOLiD sequences yielding a total of 16 microRNAs as differentially expressed by TCDD in zebrafish embryos. However, miR-27e was the only microRNA to be identified as differentially expressed by all three methods (both microarrays, SOLiD sequencing, and real-time RT-PCR). These results suggest that TCDD exposure causes modest changes in expression of microRNAs, including some (miR-451, 23a, 23b, 24 and 27e) that are critical for hematopoiesis and cardiovascular

  18. Bibliographie

    OpenAIRE

    2014-01-01

    Alantar, Ö. Zeynep, “Türk Dış Politikasında Milletler Cemiyeti Dönemi”, dans Sönmezoğlu (Faruk) (éd.), Türk Dış Politikası Analizi, Istanbul, Der Yay., 1994. Al-Daqûqi, Ibrahim, “Palestine & Zionism in the Turkish mass media”, The Third Arab-Turkish Relations Conference (abstracts of papers), Yarmouk University, 25-28 avril 1985, cité dans Duclos, Louis-Jean, « Arabes et Turquie : le cas Jordanien », CEMOTI, 1991, No. 11, p. 119-139. Aktar, Ayhan & Özel, Soli, “Turkish Attitudes vis- à-vis th...

  19. Selective packaging of cellular miRNAs in HIV-1 particles.

    Science.gov (United States)

    Schopman, Nick C T; van Montfort, Thijs; Willemsen, Marcel; Knoepfel, Stefanie A; Pollakis, Georgios; van Kampen, Antoine; Sanders, Rogier W; Haasnoot, Joost; Berkhout, Ben

    2012-11-01

    Retroviral particles are known to package specific host cell components such as RNA molecules in addition to the two copies of the viral RNA genome. The highly sensitive SOLiD sequencing technology was used to determine the cellular miRNA content of human immunodeficiency virus type 1 (HIV-1) particles. We determined the relative concentration of cellular miRNAs in a T cell line and several primary cell subsets before and after HIV-1 infection, and compared those values to the miRNA content of virion particles. A small subset of the cellular miRNAs is dramatically concentrated in the virions up to 115 fold, suggesting a biological function in HIV-1 replication. PMID:22728443

  20. 测序新技术Ion Torrent PGM及其法医学应用%The new sequencing technology-Ion Torrent PGM and its forensic application

    Institute of Scientific and Technical Information of China (English)

    刘宇轩; 张文琼; 黄代新

    2015-01-01

    目前,基因测序技术发展迅速,继Roche公司的454系统、Illumina公司的HiSeq 2000系统和AB公司的 SOLiD系统之后,Life Technologies公司于2010年推出了Ion Torrent Personal G enome Machine (PGM)测序仪.Ion Torrent是基于半导体芯片的测序技术,不属于核酸标记、荧光检测的生化技术.与其他测序技术相比,操作简便,费用较低,具有强大的扩展性.本文就Ion Torrent PGM的特点及其在法医学中的应用进行简要介绍.

  1. Individual Genome of the Russian Male: SNP Calling and a de novo Assembly of Unmapped Reads.

    Science.gov (United States)

    Chekanov, N N; Boulygina, E S; Beletskiy, A V; Prokhortchouk, E B; Skryabin, K G

    2010-07-01

    A somatic cell genome was recently resequenced for a patient with renal cancer. The data were submitted to the NCBI Sequence Read Archive under the accession number SRA012240. Here, we have performed SNP calling for the genome and compared it with several published genomes. We have found 2, 921, 724 SNPs, including 1, 472, 679 newly described ones. Among them, 63, 462 SNPs have been mapped to the Y chromosome and, based on 18 markers, the genome has been ascribed to the R1a1a haplogroup predominant in Russian males. The mitochondrial haplogroup has been determined as U5a, which is also common in the European part of Russia. Short reads unmapped to the human genome were used for thede novoassembly of DNA sequences. This resulted in genome-specific contigs (more than 100 bp in length) with an overall length of 154 kbp (for GAII) and 4.7 kbp (for SOLiD).

  2. Caso para diagnóstico Case for diagnosis

    OpenAIRE

    Heliane Sanae Suzuki; Leila Cristina Cavalin; Betina Werner; Maurício Shigueru Sato; Fabiane Mulinari Brenner

    2007-01-01

    Piloleiomioma é tumor benigno de músculo liso que tem origem no músculo eretor do pêlo. Pode ocorrer isoladamente ou em grande número. Apresenta-se doloroso, sensível ao frio, toque, pressão e emoção. Relata-se caso de um homem com nódulos intradérmicos compostos por fibras de músculo liso. A imuno-histoquímica mostra-se positiva para desmina e actina, confirmando o diagnóstico.Piloleiomyoma is a benign smooth muscle tumor which arises from the erector pilorum muscles. The lesions can be soli...

  3. Bisector Area: A New Metric for Calculating Spectral Line Bisectors

    Science.gov (United States)

    Evans, Kaitlin; Harker, Brian

    2016-05-01

    We present a study of a new metric for characterizing spectral line bisectors from integrated Sun-as-a-star measurements of photospheric spectral lines. This metric, which we call bisector area, differs from previous analysis methods in that it characterizes the entire bisector (up to a maximum intensity limit) with a single scalar quantity. In this preliminary study, we analyzed data from the Synoptic Optical Long-term Investigations of the Sun Integrated Sunlight Spectrometer (SOLIS/ISS) during the decline and rise of solar cycles 23 and 24, respectively, to diagnose any potential correlations between bisector area and other known changes in spectral line properties as a function of time. This work was carried out through the National Solar Observatory Research Experiences for Undergraduates (REU) Program, which is funded by the National Science Foundation (NSF). The National Solar Observatory is operated by the Association of Universities for Research in Astronomy, Inc. (AURA) under cooperative agreement with the NSF.

  4. Fare astronomia con piccoli telescopi

    CERN Document Server

    Gainer, Michael K

    2007-01-01

    Non sono necessariamente richiesti strumenti mastodontici per produrre risultati scientificamente validi nel campo dell’astronomia. Anche l’astrofilo dotato di un piccolo telescopio, con un diametro di soli 8-9 cm, può contribuire alla scienza del cielo realizzando utili osservazioni del Sole, della Luna, dei pianeti, delle comete, degli asteroidi, delle stelle doppie o variabili, delle nebulose e degli ammassi stellari. Il manuale di M.K. Gainer spiega quale sia la dotazione minima (un piccolo telescopio, un computer, una semplice fotocamera digitale), come utilizzarla, e quali siano le tecniche appropriate da adottare nelle osservazioni. Offre inoltre schemi per interpretare e ridurre i dati raccolti, nonché schede da compilare e da spedire ai centri di raccolta internazionali. Questo libro è il passaporto grazie al quale l’astrofilo può entrare a pieno titolo nel mondo affascinante della scienza astronomica.

  5. Magnetic and density spikes in cosmic ray shock precursors

    CERN Document Server

    Malkov, M A; Diamond, P H

    2011-01-01

    In shock precursors populated by accelerated cosmic rays (CR), the CR return current instability is believed to significantly enhance the pre-shock perturbations of magnetic field. We have obtained fully-nonlinear exact ideal MHD solutions supported by the CR return current. The solutions occur as localized spikes of circularly polarized Alfven envelopes (solitons, or breathers). As the conventional (undriven) solitons, the obtained magnetic spikes propagate at a speed $C$ proportional to their amplitude, $C=C_{A}B_{{\\rm max}}/\\sqrt{2}B_{0}$. The sufficiently strong solitons run thus ahead of the main shock and stand in the precursor, being supported by the return current. This property of the nonlinear solutions is strikingly different from the linear theory that predicts non-propagating (that is, convected downstream) circularly polarized waves. The nonlinear solutions may come either in isolated pulses (solitons) or in soliton-trains (cnoidal waves). The morphological similarity of such quasi-periodic soli...

  6. Carrying Out the Comprehensive Utilization of Mineral Solid Waste and Promoting the Sustainable Development of Mining%开展固体废弃物综合利用促进矿业可持续发展

    Institute of Scientific and Technical Information of China (English)

    常前发

    2001-01-01

    从4个方面分析了矿产固体废弃物综合利用的必要性和迫切性,认为矿产固体废弃物综合利用是矿业可持续发展的必然要求,市场潜力巨大,最后指出了矿产固体废弃物综合利用的方向。%An analysis is made from four aspe cts of the necessity andurgency of the comprehensive utilization of mineral sol id waste and it is considered that the comprehensive utilization of mineral soli d waste is an inevitable requirement of the sustainable development of mining an d has great market potentiality.Its development orientation is pointed out and s ome suggestions are made.

  7. Adverse Effect of H2O2 Change on Morphology, Mitochondrial Membrane Permeability and Antioxidant Enzyme in Root of Dianthus Chinensis L. under Salt Stress

    Directory of Open Access Journals (Sweden)

    Xue-qin He

    2013-04-01

    Full Text Available Dianthus Chinensis L. is a salt-tolerant ornamental plant. Root is the first and critical part of plant to encounter soli salinity. In order to elucidate H2O2 impact on root morphology and mitochondrial permeability transition as well as activities of antioxidant enzymes in root ofDianthus Chinensis L., we treated seedling with H2O2 and DMTU under NaCl. The results revealed that change of H2O2 level under NaCl would negatively influence the root growth, as well as lower the value of mitochodrial membrane absorbance at 540 nm and the ratio of Cyt c/a. Meanwhile, SOD and POD under NaCl plus H2O2 and NaCl plus DMTU were far lower than those under NaCl alone.

  8. Stable dark and bright soliton Kerr combs can coexist in normal dispersion resonators

    CERN Document Server

    Parra-Rivas, P; Gelens, L

    2016-01-01

    Using the Lugiato-Lefever model, we analyze the ef- fects of third order chromatic dispersion on the exis- tence and stability of dark and bright soliton Kerr fre- quency combs in the normal dispersion regime. While in the absence of third order dispersion only dark soli- tons exist over an extended parameter range, we find that third order dispersion allows for stable dark and bright solitons to coexist. Reversibility is broken and the shape of the switching waves connecting the top and bottom homogeneous solutions is modified. Bright solitons come into existence thanks to the generation of oscillations in the switching wave profiles. Finally, oscillatory instabilities of dark solitons are also sup- pressed in the presence of sufficiently strong third or- der dispersion.

  9. The reversal of the Sun's magnetic field in cycle 24

    CERN Document Server

    Mordvinov, Alexander V; Bertello, Luca; Petrie, Gordon J D

    2016-01-01

    Analysis of synoptic data from the Vector Stokes Magnetograph (VSM) of the Synoptic Optical Long-term Investigations of the Sun (SOLIS) and the NASA/NSO Spectromagnetograph (SPM) at the NSO/Kitt Peak Vacuum Telescope facility shows that the reversals of solar polar magnetic fields exhibit elements of a stochastic process, which may include the development of specific patterns of emerging magnetic flux, and the asymmetry in activity between northern and southern hemispheres. The presence of such irregularities makes the modeling and prediction of polar field reversals extremely hard if possible. In a classical model of solar activity cycle, the unipolar magnetic regions (UMRs) of predominantly following polarity fields are transported polewards due to meridional flows and diffusion. The UMRs gradually cancel out the polar magnetic field of the previous cycle, and re-build the polar field of opposite polarity setting the stage for the next cycle. We show, however, that this deterministic picture can be easily a...

  10. VPLIV SKUPNEGA IONA NA VISKOZNOST ELEKTROLITSKIH MEŠANIC

    OpenAIRE

    Hiter, Marijana

    2016-01-01

    S Cannon-Fenskejevim viskozimetrom v termostatirani celici smo merili vpliv skupnega iona na viskoznost elektrolitskih mešanic. Meritve so bile opravljene pri temperaturah 20°C, 25°C, 30°C, 35°C, 40°C, 45°C in 50°C. Za merjenje viskoznosti smo si pripravili čiste raztopine KCl, NaCl in NaNO3 s koncentracijo 0,1 mol/L. Pripravili smo mešanice soli, s koncentracijami od 0,01 do 0,09 mol/L tako, da je bila skupna koncentracija vedno 0,1 mol/L. V raztopini KCl in NaCl smo preučevali vpliv skupneg...

  11. Corynebacterium pseudotuberculosis RNA-seq data from abiotic stresses.

    Science.gov (United States)

    de Sá, Pablo H C G; Veras, Adonney A O; Carneiro, Adriana R; Barúna, Rafael A; Guimarães, Luís C; Pinheiro, Kenny C; Pinto, Anne C; Soares, Siomar C; Schneider, Maria P C; Azevedo, Vasco; Silva, Artur; Ramos, Rommel T J

    2015-12-01

    Corynebacterium pseudotuberculosis causes significant loss to goat and sheep farmers because it is the causal agent of the infectious disease caseous lymphadenitis, which may lead to outcomes ranging from skin injury to animal death (Ruiz et al., 2011) [1]. This bacterium was grown under osmotic (2 M), acid (pH) and heat (50 °C) stress and under control (Normal-BHI brain heart infusion) conditions, which simulate the conditions faced by the bacteria during the infectious process. Subsequently, cDNA of each condition was sequenced by the SOLiD3 Plus platform using the RNA-Seq technique [2], [3], [4]. The data produced was processed to evaluate the differential gene expression, which is helpful to understand the adaptation mechanisms during the infection in the host. The sequencing data of all conditions are available in the European Bioinformatics Institute (EBI) repository under accession number E-MTAB-2017. PMID:26702428

  12. Corynebacterium pseudotuberculosis RNA-seq data from abiotic stresses

    Directory of Open Access Journals (Sweden)

    Pablo H.C.G. de Sá

    2015-12-01

    Full Text Available Corynebacterium pseudotuberculosis causes significant loss to goat and sheep farmers because it is the causal agent of the infectious disease caseous lymphadenitis, which may lead to outcomes ranging from skin injury to animal death (Ruiz et al., 2011 [1]. This bacterium was grown under osmotic (2 M, acid (pH and heat (50 °C stress and under control (Normal-BHI brain heart infusion conditions, which simulate the conditions faced by the bacteria during the infectious process. Subsequently, cDNA of each condition was sequenced by the SOLiD3 Plus platform using the RNA-Seq technique [2–4]. The data produced was processed to evaluate the differential gene expression, which is helpful to understand the adaptation mechanisms during the infection in the host. The sequencing data of all conditions are available in the European Bioinformatics Institute (EBI repository under accession number E-MTAB-2017.

  13. Identification of microsatellites from an extinct moa species using high-throughput (454) sequence data

    DEFF Research Database (Denmark)

    Allentoft, Morten Erik; Schuster, Stephan C.; Holdaway, Richard N.;

    2009-01-01

    capable of generating up to 1 gigabases per run as short (200-400-bp) read lengths. 454 data were generated from the fossil bone of an extinct New Zealand moa (Aves: Dinornithiformes). We identified numerous short tandem repeat (STR) motifs, and here present the successful isolation and characterization...... of one polymorphic microsatellite (Moa_MS2). Primers designed to flank this locus amplified all three moa species tested here. The presented method proved to be a fast and efficient way of identifying microsatellite markers in ancient DNA templates and, depending on biomolecule preservation, has...... the potential of enabling high-resolution population genetic studies of extinct taxa. As sequence read lengths of the 454 platforms and its competitors (e.g., the SOLEXA and SOLiD platforms) increase, this approach will become increasingly powerful in identifying microsatellites in extinct (and extant...

  14. Evaluation de l'activité antimicrobienne de quelques pyridones de synthèse

    OpenAIRE

    OUGGAD, Hayet

    2015-01-01

    Notre travail s'inscrit dans le cadre de l'évaluation de l'activité antibactérienne et antifongique de quelques dérivés pyridones de synthèse préparés par l'équipe du professeur Chekchou-Braham Noureddine au laboratoire de catalyse et synthèse en chimie organique (LCSCO) Faculté des Science de la Nature et de la vie, Science de la terre et de l'Univers, Université Aboubekr Belkaïd Tlemcen. Cette étude a été réalisée à l'aide de la méthode de diffusion de disques sur milieu soli...

  15. Study of organic N transformation in red soils by 15N tracer method

    Institute of Scientific and Technical Information of China (English)

    YeQing-Fu; ZhangQin-Zheng; 等

    1997-01-01

    Uniformly 15N-labelled ryegrass was used to investigate NH4+-production,microbial transformation and humification of organic N in two types of red soils by incubating the soils amended with labelled material.The results showed that there was little significant difference in biomass N transformation in the tested solis between 15N tracer method and conventional method,but the amount of NH4++-N released form the ryegrass in the clayey soil than in the sandy soil at all sampling time .By 120d of incubation,humified N was less than 10% of the amount of the applied N in two types of red soils and the amount of residual N in the clayey red soil was obviously higher than that in the sandy red soil.

  16. El Hombre, un ser integral

    Directory of Open Access Journals (Sweden)

    Gabriel Alexander Solórzano

    2008-12-01

    Full Text Available El artículo pretende mostrar la dificultad que existe al momento de elaborar una precisa definición del hombre como ser integral, para ello se recurre a las nociones esenciales de la antropología filosófica, la nueva concepción del giro cristia- no de la fenomenología francesa, se mira grosso modo la visión personalista de Juan Pablo II sobre el hombre y la visión integradora de Panikkar del humanismo cosmote- ándrico, por último se encuentra la visión del humanismo cristiano y el desarrollo soli- dario enfatizado en el Magisterio de la Iglesia Católica como intento de respuesta a la crucial pregunta sobre el hombre y su mundo relacional. Palabras clave: Filosofía, Teología moral, Fenomenología, Humanismo, Perso- nalismo, Cosmoteándrico. 

  17. An evaluation of Bradfordizing effects

    CERN Document Server

    Mayr, Philipp

    2008-01-01

    The purpose of this paper is to apply and evaluate the bibliometric method Bradfordizing for information retrieval (IR) experiments. Bradfordizing is used for generating core document sets for subject-specific questions and to reorder result sets from distributed searches. The method will be applied and tested in a controlled scenario of scientific literature databases from social and political sciences, economics, psychology and medical science (SOLIS, SoLit, USB Koeln Opac, CSA Sociological Abstracts, World Affairs Online, Psyndex and Medline) and 164 standardized topics. An evaluation of the method and its effects is carried out in two laboratory-based information retrieval experiments (CLEF and KoMoHe) using a controlled document corpus and human relevance assessments. The results show that Bradfordizing is a very robust method for re-ranking the main document types (journal articles and monographs) in today's digital libraries (DL). The IR tests show that relevance distributions after re-ranking improve ...

  18. O clima como necessidade de governança transnacional: reflexões pós-Copenhague 2009

    OpenAIRE

    Paulo Márcio Cruz; Zenildo Bodnar

    2010-01-01

    Resumo: O presente artigo aborda a questão climática analisada sob a ótica da governança transnacional como necessidade para enfrentar os desafios pós XV Conferência da Mudança do Clima da ONU (COP 15), Copenhague 2009. Parte-se da hipótese de que não é possível o estabelecimento de uma política regulatória efetiva para a questão climática que não leve em conta a governança transnacional, entendida esta como forma de articulação entre o poder local e o global, baseada na cooperação e na soli...

  19. Références

    OpenAIRE

    2014-01-01

    Abdulkarim A., 1994 — La diaspora libanaise : une organisation communautaire. L’espace géographique, 23 (3) : 217-223. Acosta-Solis M., 1966 — « Problems of Conservation and Economic Development of the Galápagos ». In Bowman R. (ed.) : 282-285. Acot P., 1988 — Histoire de l’écologie. Paris, PUF, 285 p. Alpert L., 1963 — « The Climate of the Galápagos Islands ». In : Galápagos Islands : a unique Area for Scientific Investigation, San Francisco, California Academy of Science, Occasional Papers ...

  20. Genomic research for important pathogenic bacteria in China

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of point.We can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug targets.The newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease.

  1. Microlunatus cavernae sp. nov., a novel actinobacterium isolated from Alu ancient cave, Yunnan, South-West China.

    Science.gov (United States)

    Cheng, Juan; Chen, Wei; Huo-Zhang, Bing; Nimaichand, Salam; Zhou, En-Min; Lu, Xin-Hua; Klenk, Hans-Peter; Li, Wen-Jun

    2013-07-01

    A Gram-positive, coccoid, non-endospore-forming actinobacterium, designated YIM C01117(T), was isolated from a soil sample collected from Alu ancient cave, Yunnan province, south-west China. Based on the 16S rRNA gene sequence analysis, strain YIM C01117(T) was shown to belong to the genus Microlunatus, with highest sequence similarity of 97.4 % to Microlunatus soli DSM 21800(T). The whole genomic DNA relatedness as shown by the DNA-DNA hybridization study between YIM C01117(T) and M. soli DSM 21800(T) had a low value (47 ± 2 %). Strain YIM C01117(T) was determined to contain LL-diaminopimelic acid with Gly, Glu and Ala amino acids (A3γ' type) in the cell wall. Whole-cell hydrolysates were found to contain glucose, galactose, mannose and ribose. The major polar lipids were determined to be phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinone system present is MK-9(H4), while the major fatty acids were identified to be anteiso-C15:0 (24.1 %), iso-C16:0 (22.3 %) and iso-C15:0 (11.4 %). The G+C content of the genomic DNA was determined to be 65.9 mol%. The chemotaxonomic and genotypic data support the affiliation of the strain YIM C01117(T) to the genus Microlunatus. The results of physiological and biochemical tests allow strain YIM C01117(T) to be differentiated phenotypically from recognized Microlunatus species. Strain YIM C01117(T) is therefore considered to represent a novel species of the genus Microlunatus, for which the name Microlunatus cavernae sp. nov. is proposed. The type strain is YIM C01117(T) (= DSM 26248(T) = JCM 18536(T)).

  2. SDHA loss of function mutations in a subset of young adult wild-type gastrointestinal stromal tumors

    International Nuclear Information System (INIS)

    A subset of KIT/PDGFRA wild-type gastrointestinal stromal tumors (WT GIST) have been associated with alteration of the succinate dehydrogenase (SDH) complex II function. A recent report identified four non-syndromic, KIT/PDGFRA WT GIST harboring compound heterozygous or homozygous mutations in SDHA encoding the main subunit of the SDH complex II. Next generation sequencing was applied on five pediatric and one young adult WT GIST, by whole exome capture and SOLiD 3-plus system sequencing. The putative mutations were first confirmed by Sanger sequencing and then screened on a larger panel of 11 pediatric and young adult WT GIST, including 5 in the context of Carney triad. A germline p.Arg31X nonsense SDHA mutation was identified in one of the six cases tested by SOLiD platform. An additional p.D38V missense mutation in SDHA exon 2 was identified by Sanger sequencing in the extended KIT/PDGFRA WT GIST patients cohort. Western blotting showed loss of SDHA expression in the two cases harboring SDHA mutations, while expression being retained in the other WT GIST tumors. Results were further confirmed by immunohistochemistry for both SDHA and SDHB, which showed a concurrent loss of expression of both proteins in SDHA-mutant lesions, while the remaining WT tumors showed only loss of SDHB expression. Germline and/or somatic aberrations of SDHA occur in a small subset of KIT/PDGFRA WT GISTs, outside the Carney’s triad and are associated with loss of both SDHA and SDHB protein expression. Mutations of the SDH complex II are more particularly associated with KIT/PDGFRA WT GIST occurring in young adults. Although pediatric GIST consistently display alterations of SDHB protein expression, further molecular studies are needed to identify the crucial genes involved in their tumorigenesis

  3. Forecasting and Real-Time Diagnostics of Solar Coronal Mass Ejections

    Science.gov (United States)

    Georgoulis, M. K.; Labonte, B. J.

    2004-12-01

    We discuss an operational, fully automated, algorithm to follow the dynamical evolution and the buildup of magnetic instabilities that give rise to coronal mass ejections (CMEs) in solar active regions. The tool relies on vector magnetic field measurements of the active region photosphere / chromosphere and performs the following tasks: (1) resolution of the 180-degree ambiguity in the magnetic field measurements and preparation for further use, (2) calculation of the magnetic forces and electric currents in the active region photosphere/chromosphere, (3) reconstruction of a magnetohydrodynamic velocity field corresponding to the measured magnetic field to calculate the buildup rate of the magnetic helicity in the active region atmosphere, and (4) estimation of the total magnetic helicity in the active region corona. We present examples showing that (I) flare- and CME-prolific active regions have much higher magnetic helicity, stronger magnetic forces and more intense cross-field electric currents than quiescent active regions, and (II) the magnetic helicity, chirality, magnetic flux, and magnetic energy of a CME can be calculated in real time from the results of the algorithm before and after the CME. As a result, we can both identify potentially eruptive areas on the visible solar disk and provide detailed quantitative diagnostics of the resulting CMEs. Additional work is required to predict the geoeffectiveness of these CMEs. For the algorithm to be useful we need full-disk, ideally uninterrupted, coverage of the solar magnetic field vector. This information will be available in a few years with the Helioseismic and Magnetic Imager (HMI) on board the Solar Dynamics Observatory (SDO; launch 2008). At the moment, full-disk vector magnetograms will be provided by the ground-based Vector Spectro-Magnetograph (VSM) of the Synoptic Optical Long-Term Investigation of the Sun (SOLIS) telescope. We will utilize the SOLIS vector magnetograms as soon as they become

  4. Planetary Systems and the Origins of Life

    Science.gov (United States)

    Pudritz, Ralph; Higgs, Paul; Stone, Jonathon

    2013-01-01

    Preface; Part I. Planetary Systems and the Origins of Life: 1. Observations of extrasolar planetary systems Shay Zucker; 2. The atmospheres of extrasolar planets L. Jeremy Richardson and Sara Seager; 3. Terrestrial planet formation Edward Thommes; 4. Protoplanetary disks, amino acids and the genetic code Paul Higgs and Ralph Pudritz; 5. Emergent phenomena in biology: the origin of cellular life David Deamer; Part II. Life on Earth: 6. Extremophiles: defining the envelope for the search for life in the Universe Lynn Rothschild; 7. Hyperthermophilic life on Earth - and on Mars? Karl Stetter; 8. Phylogenomics: how far back in the past can we go? Henner Brinkmann, Denis Baurain and Hervé Philippe; 9. Horizontal gene transfer, gene histories and the root of the tree of life Olga Zhaxybayeva and J. Peter Gogarten; 10. Evolutionary innovation versus ecological incumbency Adolf Seilacher; 11. Gradual origins for the Metazoans Alexandra Pontefract and Jonathan Stone; Part III. Life in the Solar System?: 12. The search for life on Mars Chris McKay; 13. Life in the dark dune spots of Mars: a testable hypothesis Eörs Szathmary, Tibor Ganti, Tamas Pocs, Andras Horvath, Akos Kereszturi, Szaniszlo Berzci and Andras Sik; 14. Titan: a new astrobiological vision from the Cassini-Huygens data François Raulin; 15. Europa, the Ocean Moon: tides, permeable ice, and life Richard Greenberg; Index.

  5. Dôchodková reforma v Čile a jej súčasné problémy

    OpenAIRE

    Bieliková, Michala

    2007-01-01

    Práca sa zaoberá rozborom dôchodkovej reformy v Čile, v ktorej ako prvej krajine na svete bol v roku 1981 priebežný dôchodkový systém nahradený povinným dôchodkovým sporením na osobných účtoch v súkromných dôchodkových spoločnostiach. Prvá časť práce popisuje možné prístupy k riešeniu nedostatkov priebežne financovaného dôchodkového systému. Druhá časť popisuje samotnú dôchodkovú reformu v Čile, od jej transformácie až po súčasné nastavenie. Tretia časť rozoberá makroekonomické dopady tejto r...

  6. Obligations Related to the Transportation of pet Animals (Eu Legislation / Povinnosti Súvisiace S Prepravou Spoločenských Zvierat (Európska Legislatíva

    Directory of Open Access Journals (Sweden)

    Pavlíková Barbara

    2015-06-01

    Full Text Available Prispevok sa zaobera otazkou, ktoru často riešia majitelia spoločenskych zvierat vo všetkych členskych štatoch EU. Poskytuje bližši pohľad na povinnosti spojene s cestovanim so zvieraťom medzi členskymi štatmi, alebo medzi členskym štatom a treťou - nečlenskou krajinou. Zameriava sa na nekomerčnu prepravu, ako je napriklad dovolenka, vylet a podobne. Praca prostrednictvom syntezy, analyzy a porovnania ponuka prehľad zakladnych legislativnych aktov v predmetnej oblasti. Venuje sa tiež otazke tzv. pet pasu, najdoležitejšieho identifikačneho dokladu, vyžadovaneho pre spoločenske zvierata na uzemi EU. Možno v nej najsť aj definicie najčastejšie použivanej terminologie.

  7. NATO, Balkán a verejná diplomacia

    OpenAIRE

    Laura Meričková

    2012-01-01

    Článok analyzuje, ako vnímajú organizáciu NATO na Balkáne a objasňuje rozdiely medzi štátmi. Následne využíva poznatky teórie konštruktivizmu a odborníkov, ktorí vysvetľujú, že negatívne postoje k NATO sa menia k pozitívnemu vďaka dlhoročnej prítomnosti organizácie a EÚ na Balkáne. Autor síce súhlasí s daným tvrdením, argumentuje však, že vzhľadom na to, že verejná mienka o NATO na Balkáne je špecifická, NATO by vo svojej verejnej diplomacii nemalo zostávať len na úrovni informačnej kampane. ...

  8. Využití umělé inteligence pro optimalizaci výroby

    OpenAIRE

    Svoboda, Radovan

    2012-01-01

    Diplomová práca rieši problematiku optimalizácie výrobného plánu s použitím genetických algoritmov. Obsahuje vysvetlenie princípu genetických algoritmov v súvislostiach s evolučnými algoritmami a umelou inteligenciou celkovo. Ďalej je rozoberaná problematika plánovania výroby a činnosti, ktoré s ním súvisia. Následne práca popisuje modifikáciu genetických algoritmov a následne ich použitie v počítačovom programe AI Planner, ktorý slúži na tvorbu a optimalizáciu výrobného plánu, ako riešenie z...

  9. Mikroprogram pre riadenie procesu spájania operandov v architektúre DF KPI

    Directory of Open Access Journals (Sweden)

    Norbert Ádám

    2013-12-01

    Full Text Available V článku uvedená architektúra je založená na výpočtovom modeli „data flow“, v ktorom riadenie výpočtu je založené na spracovaní toku dát. Základným prvkom DF architektúry je koordinačný procesor (CP, ktorý je zodpovedný za riadenie a organizáciu vykonávania inštrukcií. Štruktúrna organizácia CP je navrhnutá ako dynamický multifunkčný systém. Pri vykonávaní operácií môže CP prechádzať rôznymi stavmi, v dôsledku čoho táto jednotka predstavuje dynamický prúdový systém. Prechod a poradie prechodu medzi jednotlivými stavmi CP je podmienený typom spracovaného operátora pri interpretovaní prúdu operandov. Z možných typov operátorov, článok uvádza mikroprogramové riadenie pre jednovstupové operátory.

  10. Rupprech Rohr, Einführung in das Studium der Romanistik, Erich Schmidt Verlag, Berlin, 1964

    Directory of Open Access Journals (Sweden)

    Žarko Muljačić

    2016-02-01

    Full Text Available U poslijeratno doba opaža se posvuda potreba da se studentima filoloških disciplina pruže upu te u struku koju su izabrali pa sli se tako i za pojedine romanske jezike i književnosti, i za romanistiku u cjelini pojavili u mnogim zemljama gel.je se ti predmeti uče i raznovrsni priručnici. Svima je cilj da, uz živu riječ nastavnika gornji o toj problematici nerijetko drže posebne kolegije, pomognu početnicima da se snadju u mnoštvu udžbenika i drugih knjiga te golemog broja znanstvenih priloga što izlaze u sve brojnijim časopisima u Evropi i van nje. Ako se zna da je preosjek znanja što ga tj.onose abiturijenti na ovom sektru opčenito niži nego prije tata, onda je shvatljivo da su neki autori takvih priručnika osjetili potrebu da svojim čitateljima pruže nešto više od šturih bibliografskih podataka. Stoga su mnoga djela preraslasažete, i te kako dobrodošle uvode u pojedine znanstvene discipline i njihove dijelove.

  11. Výroba kapalných biopaliv s využitím enzymatické hydrolýzy odpadního papíru

    OpenAIRE

    Roštek, Martin

    2015-01-01

    Táto bakalárska práca sa zaoberá rešeršným zhrnutím aktuálnych poznatkov o procese enzymatickej hydrolýzy, s vyžitím odpadového papiera ako suroviny, za cieľom jeho konverzie na kvapalné biopalivo. V rámci práce sú zhrnuté všeobecné poznatky o lignocelulózových surovinách a ich zdrojoch, prehľad dostupných metód hydrolýzy pre dané materiály a fermentačné technológie, a tiež sumarizácia súčasne využívaných metód zefektívňujúcich proces enzymatickej hydrolýzy. V ďalšej časti práce sa nachádza p...

  12. Webová platforma pro tvorbu her

    OpenAIRE

    Gulán, Filip

    2016-01-01

    Cieľom tejto práce je návrh a realizácia modernej webovej platformy pre tvorbu hier. Webová platforma je určená, ako pre vývojárov HTML5/Javascript hier, tak aj pre hráčov týchto hier. Súčasťou práce je okrem webového portálu aj API, ktoré sprístupňuje služby webového portálu vývojárom hier. Táto platforma je na serverovej strane postavená na skriptovacom jazyku PHP za použitia rámca Nette. Na klientskej strane je využité HTML, CSS, Javascript a pre účely responzívneho dizajnu je využívaný fr...

  13. Obsah vybraných biologicky aktivních látek ve Stévii cukerné

    OpenAIRE

    Porubčanová, Vladimíra

    2013-01-01

    Táto bakalárska práca sa zaoberá rastlinou, nazývanou stévia sladká a analýzou fenolických látok v extraktoch z nej. Teoretická časť zahŕňa botanický popis rastliny, jej chemické zloženie i vplyv na ľudské zdravie. Zaoberá sa tiež sladidlom vyrobeným zo stévie, označeným ako E 960: steviol-glykozidy. V práci je uvedený i zoznam firiem, ktoré vyrábajú toto sladilo, vrátane potravinárskych výrobkov, v ktorých je použité. Experimentálna časť je zameraná na stanovenie fenolických látok v extrakto...

  14. Organizacija tehnološkog procesa remonta složenih tehničkih sistema u tehničkom remontnom zavodu u Čačku

    Directory of Open Access Journals (Sweden)

    Vojkan M. Radonjić

    2014-04-01

    Full Text Available Složeni tehnički sistemi (STS od ostalih tehničkih sistema (TS razlikuju se po konstrukciji i nameni, koja može biti: zaštita ljudi, materijalnih bogatstava i teritorije. Ako želimo da STS budu ispravni i imaju zadovoljavajući nivo pouzdanosti i gotovosti,  sistem remonta mora biti u stanju da podrži zahteve koji proističu iz njihove specifičnosti. Sistem njihovog remonta mora biti programiran, a upravljanje njime zasnovano na konceptu QMS (Quality management system. Organizacija remonta STS za potrebe sistema odbrane Republike Srbije, u Tehničkom remontnom zavodu u Čačku (TRZ, primer je organizacije u koju je implementiran  procesni model sistema upravljanja kvalitetom (QMS. Ovaj članak opisuje proces remonta STS u TRZ sa njegovim procesnim sistemom upravljanja kvalitetom (QMS, koji je preduslov za efikasan i efektivan proces remonta. Proces remonta prikazan je na primeru remonta guseničnog borbenog vozila. 

  15. Identifikace objektů

    OpenAIRE

    Fábry, Tomáš

    2011-01-01

    Práca popisuje tvorbu a funkčnosť vytvoreného programu na identifikáciu objektov. Program vychádza zo snímky z web kamery a predloženého vzoru hľadaného objektu. Rozpoznáva všetky objekty na snímke a označí tie podobné vzoru spolu s odchýlkami od neho. Program je vytvorený ako aplikácia pre Windows pomocou jazyka C/C++. Pre komunikáciu s web kamerou a zobrazovanie výsledkov sú použité funkcie knižnice OpenCV. V práci je ukázaná štruktúra programu a usporiadanie dát. Sú tu ďalej popísané najdô...

  16. Obchodovanie na svetových menových trhoch Forex - trading a jeho základné piliere

    OpenAIRE

    Jozef Portašík

    2009-01-01

    Tento článok sa venuje obchodovaniu na svetových menových trhoch Forex, ktorý sa nazýva trading. Venuje sa taktiež aj jeho základným pilierom, ktoré sú nepostrádateľnou súčasťou obchodovania každého úspešného obchodníka - tradera. Jedná sa o objasnenie pohľadu na trading ako na biznis. Rozoberá trading z jeho pozitívnych stránok, ale aj varuje pred možnými rizikami. Ďalej sa zaoberá základnými piliermi každého úspešného obchodníka. Jedná sa o tri hlavné piliere. Prvým je psychológia a mysleni...

  17. Využití analýz pro intradenní obchodování na mezinárodním měnovém trhu

    OpenAIRE

    Radošinský, Martin

    2016-01-01

    Predmetom diplomovej práce je analyzovať možnosti obchodovania na Forexe s využitím kombinácie fundamentálnej a technickej analýzy vo väzbe na intradenné obchodovanie. Jedným z cieľov je identifikovať výhody a nevýhody využitia jednotlivých analýz. Na základe získaných poznatkov navrhnúť obchodné portfólio zložené z viacerých stratégií, pričom jednotlivé stratégie budú naprogramované ako AOS a optimalizované a otestované na historických cenových dátach. The main aim of this diploma thesis ...

  18. Termoplastické kompozity pro automobilové aplikace

    OpenAIRE

    Zbončák, Marek

    2014-01-01

    Diplomová práca sa zaoberá prípravou teromplastických kompzoitov s PMMA a PC matricou s potenciálnym využitím v automobilovom priemysle. Ako výstuž boli použité krátke sklenené, uhlíkové a PBO (poly(p-fenylén benzobisoxazol)) vlákna známe pod obchodným názvom Zylon®. Práve do PBO vlákien boli vkladané veľké nádeje vzhľadom na ich ohromujúce mechanické vlastnosti. Vplyv objemového zlomku vlákien na modulu pružnosti, pevnosť a ťažnosť kompozitov bol skúmaný. Experimentálne zistený modul pružnos...

  19. Analýza bezpečnosti bezdrátových sítí

    OpenAIRE

    Bencel, Jozef

    2009-01-01

    Práca sa zaoberá bezdrôtovými sieťami štandardu 802.11 a možnosťami ich zabezpečenia. Sú popísané spôsoby zabezpečenia ako skrývanie SSID, filtrácia MAC adries, protokoly WEP, WPA, WPA2. Popísané sú aj najčastejšie spôsoby útokov na tieto siete (DoS, odhalenie WEP hesla, Man-in-the-Middle). Súčasťou práce je prieskum bezdrôtových sietí v oblasti Brna poukazujúci na používaný spôsob zabezpečenia (WEP, WPA, WPA2) v týchto sieťach. Použité aplikácie pre meranie boli NetStumbler a InSSIDer. Ďalšo...

  20. OSOBNI STEČAJ U HRVATSKOJ I SLOVENIJI

    Directory of Open Access Journals (Sweden)

    Sime Ivanjko

    2014-12-01

    Full Text Available Postupak osobnog stečaja bez obzira na pojedina dobra ili slaba rješenja u bilo kojoj državi neće naići na opću suglasnost u društvu, a osobitom u onim društvima gdje se svako neplaćanje dugova ocjenjuje negativnim moralnim predznakom i bez obzira na realne mogućnosti dužnika da plaća svoje dugove. Posebno to važi za neplaćanje obveza u poduzetništvu. Poduzetništvo je po svojoj osnovi amoralno - što znači da ga nije moguće ocjenjivati pozitivnim ili negativnim moralnim predznakom ako se poštuju pravila poduzetništva. Tako npr. stečaj gospodarskog subjekta nije u osnovi nemoralan jer je to nužna pojava u kapitalizmu. U prosjeku je potrebno da oko 300 sudionika u poduzetništvu na njih 10 000 mora u stečaj jer inače kapitalistički sustav ne može djelovati i ne može biti efikasan. U socijalizmu nismo imali stečajeva i zato poduzetništvo nije moglo biti konkurentno s partnerima u kapitalističkim sustavima.

  1. Řízení zkušenosti zákazníků

    OpenAIRE

    Seifert, Marián

    2014-01-01

    Cieľom diplomovej práce je analýza aktuálnej situácie zákazníckych skúseností vytváraných spoločnosťou a poskytnutie sérií odporúčaní pre ich zlepšenie. Práca kriticky hodnotí praktiky ovplyvňujúce zákaznícku spokojnosť v jednotlivých predajňách spoločnosti, internetovom obchode, servisnom stredisku a zákazníckom centre. Výsledkom sú odporúčané riešenia pre poskytovanie lepších služieb na zvýšenie zákazníckej loajality, ako aj spôsoby eliminácie zistených nedostatkov. The purpose of the th...

  2. Marketing osobnosti

    OpenAIRE

    Šušnyák, Viktor

    2008-01-01

    Táto práca sa zaoberá problematikou osobných značiek a využitia celebrít v reklame. Cieľom je identifikovať hlavné prvky pôsobiace na image osobnosti a prepojiť závery s možnosťami a rizikom využitia slávnych osobností ako doporučovateľov v reklame. Práca je rozdelená do troch častí. Témou prvej časti je self-marketing a celebrity PR, teda budovanie osobnej značky. Druhá sa zaoberá využitím už vybudovaných osobných značiek pre propagáciu iných produktov. V tretej sú prezentované a analyzované...

  3. Software pro hledání zranitelných počítačů v síti

    OpenAIRE

    Krkoš, Radko

    2009-01-01

    Práca sa zaoberá bezpečnosťou počítačových systémov a sieťovej infraštruktúry. Opisuje problematiku bezpečnostných dier a zranitežností a diskutuje možnosti ochrany pred počítačovými útokmi. Popísáné sú najčastejšie bezpečnostné diery a chyby v nastaveniach bežných klientských a serverových aplikácií. Uvedené sú jednoduché rady a postupy ako nastaviť prostredie s ohžadom na bezpečnosť a minimalizovať riziko zlyhania či narušenia systému kvôli bezpečnostným chybám a dieram. Práca sa tiež venuj...

  4. The saker falcon (Falco cherrug population, diet and nest boxes in Slovakia: LIFE-project report 2011–2014

    Directory of Open Access Journals (Sweden)

    Chavko Jozef

    2014-12-01

    Full Text Available Od októbra 201 0 do decembra 201 4 bol realizovaný projekt LIFE09 NAT/HU/000384 v štyroch Chránených vtácích územiach na Slovensku. Projekt nadviazal na predchádzajúci LIFE projekt, realizovaný v rokoch 2006 až 201 0. Oba projekty podporovali pravidelné sledovanie populácie sokola rároha a nové prístupy pre zachovanie druhu. Projekt LIFE09 prispel k zvýšeniu poznatkov o zložení potravy a rovnako aj o správaní sa tohto druhu. Populácia bola silne ovplyvnená v roku 201 3 mimoriadne zlými poveternostnými podmienkami pocasia (1 ,79 vyleteného mládata na zapocaté hniezdenie. Aj ked sa predpokladá, že celková populácia je vyššia, v roku 201 4 bolo zistených na Slovensku spolu 31 párov sokola rároha. Analýzou potravy (2991 jedincov, bolo identifikovaných 47 druhov koristi, z ktorých vtáky boli dominantné (80 %. Viac ako polovicu potravy tvoril holub domáci (Columba livia f. domestica (59 %

  5. Reducing urban diffuse pollution and surface water flooding using retrofit street trees

    Science.gov (United States)

    Rothwell, James; Stringer, Pete; Causer, Katherine; Ryan, Matt; Mangan, Steve; Appleton, Ian; Savage, Mike

    2016-04-01

    Nature-based solutions for the management of urban stormwater have been growing in popularity, but there is a lack of empirical performance data for field-scale installations, especially in a UK context. To address this deficiency, a novel retrofit street tree demonstration project was commissioned in the City of Salford, near Manchester (UK). Three fifteen year-old London Plane trees were planted within a large roadside tree trench on an urban residential street. The DeepRoot Silvia Cell modular suspended pavement system was used to maximise soil volume, avoid compaction and support large tree growth. Road runoff is directed to the tree trench via AKO Slot Kerbs. Water is then distributed evenly throughout the whole system via a perforated pipe. Excess water is conveyed out of the system via an underdrain, which is subsequently connected to the sewer network. The tree trench is lined with an impermeable membrane. Access chambers are positioned on the inflow and outflow of the tree trench to facilitate hydrological and water quality monitoring. Installation was completed in autumn 2015 and monitoring will be conducted over a three year period. This paper will provide an overview of the installation process and present initial results on the pollutant removal performance and hydrological functioning of the system.

  6. Návrh technologické jednotky pro odstraňování perzistentních látek při termickém zpracování kontaminované biomasy

    OpenAIRE

    Plch, Michal

    2009-01-01

    Diplomová práca sa zaoberá návrhom metódy čistenia spalín pre spažovňu biomasy, ktorá zaručí odstránenie dioxínov zo spalín. Popisuje metódy čistenia spalín, ako je ADIOX, použitie keramických filtrov, katalytickej filtrácie REMEDIA a metódy DeNOx/DeDiox. Porovnáva jednotlivé metódy z hžadiska účinnosti a podža ekonomickej analýzy aplikovanej na spažovňu biomasy s kotlom o výkone 1 MW. Súčasťou práce je oboznámenie sa s pojmom biomasa a s jej termickým spracovaním, pri ktorom vznikajú dioxíny...

  7. Identification and Pharmacological Analysis of High Efficacy Small Molecule Inhibitors of EGF-EGFR Interactions in Clinical Treatment of Non-Small Cell Lung Carcinoma: a Computational Approach.

    Science.gov (United States)

    Gudala, Suresh; Khan, Uzma; Kanungo, Niteesh; Bandaru, Srinivas; Hussain, Tajamul; Parihar, Ms; Nayarisseri, Anuraj; Mundluru, Hema Prasad

    2015-01-01

    Inhibition of EGFR-EGF interactions forms an important therapeutic rationale in treatment of non-small cell lung carcinoma. Established inhibitors have been successful in reducing proliferative processes observed in NSCLC, however patients suffer serious side effects. Considering the narrow therapeutic window of present EGFR inhibitors, the present study centred on identifying high efficacy EGFR inhibitors through structure based virtual screening strategies. Established inhibitors - Afatinib, Dacomitinib, Erlotinib, Lapatinib, Rociletinib formed parent compounds to retrieve similar compounds by linear fingerprint based tanimoto search with a threshold of 90%. The compounds (parents and respective similars) were docked at the EGF binding cleft of EGFR. Patch dock supervised protein-protein interactions were established between EGF and ligand (query and similar) bound and free states of EGFR. Compounds ADS103317, AKOS024836912, AGN-PC-0MXVWT, GNF-Pf-3539, SCHEMBL15205939 were retrieved respectively similar to Afatinib, Dacomitinib, Erlotinib, Lapatinib, Rociletinib. Compound- AGN-PC-0MXVWT akin to Erlotinib showed highest affinity against EGFR amongst all the compounds (parent and similar) assessed in the study. Further, AGN-PC-0MXVWT brought about significant blocking of EGFR-EGF interactions in addition showed appreciable ADMET properties and pharmacophoric features. In the study, we report AGN-PC-0MXVWT to be an efficient and high efficacy inhibitor of EGFR-EGF interactions identified through computational approaches.

  8. O českých a slovenských metódach vyhnutia sa udržateľnému rozvoju

    Directory of Open Access Journals (Sweden)

    Mikuláš Huba

    2007-12-01

    Full Text Available Doteraz som svojim príspevkom zvykol dávať prevažne optimistické názvy ako napríklad: Smerom k TU budúcnosti, Smerovanie k udržateľnému/udržateľnejšiemu regiónu XY a podobne. Myslím, že dozrel čas, stručne analyzovať metódy, ktoré tzv. kompetentní v oboch našich nástupníckych republikách používajú na to, aby sa smerovaniu k udržateľnejšej budúcnosti vyhli. Tie metódy sú na Slovensku a v Česku v niečom podobné, a v niečom sa líšia.

  9. Resposta taquicárdica e controle autonômico no exercício físico em modelo genético de insuficiência cardíaca Tachycardic response and autonomic control in physical exercise in genetic model of cardiac insufficiency

    Directory of Open Access Journals (Sweden)

    Telma F. Cunha

    2009-08-01

    Full Text Available O aumento da atividade nervosa simpática e a taquicardia em repouso ou durante esforços físicos estão associados ao aumento da morbimortalidade, mesmo na ausência de sinais clínicos de doença cardíaca. Sabendo-se da importância dos receptores α2A/α2C-adrenérgicos na modulação da atividade nervosa e frequência cardíaca (FC, o presente trabalho utiliza um modelo genético de cardiomiopatia induzida por excesso de catecolaminas circulantes baseado na inativação gênica dos receptores α2A/α2C-adrenérgicos em camundongos (α2A/α2CKO para verificar a resposta da FC ao exercício físico (EF, assim como o controle simpatovagal da FC ao EF. Testou-se a hipótese de que haveria resposta taquicárdica exacerbada durante o EF nos camundongos α2A/α2CKO mesmo quando a função cardíaca ainda estivesse preservada em repouso, sendo o receptor α2A-adrenérgico o principal responsável por essa resposta. Camundongos machos da linhagem C57Bl6J, controle (CO e com inativação gênica para os receptores α2A (α2AKO, α2C α2CKO e α2A/α2CKO foram submetidos a um teste de tolerância ao esforço físico. Outros dois grupos de camundongos, CO e α2A/α2CKO, foram submetidos ao bloqueio farmacológico dos receptores muscarínicos e β-adrenérgicos e ao EF progressivo para se avaliar a contribuição simpatovagal para a taquicardia de EF. Observou-se intolerância ao esforço físico (1.220 ± 18 e 1.460 ± 34 vs. 2.630 ± 42m, respectivamente e maior taquicardia ao EF (765 ± 16 e 792 ± 13 vs. 603 ± 18bpm, respectivamente nos camundongos α2AKO e α2A/α2CKO vs. CO. Além disso, o balanço autonômico estava alterado nos camundongos α2A/α2CKO pela hiperatividade simpática e menor efeito vagal cardíaco. Esses resultados demonstram a importância dos receptores α2A/α2C-adrenérgicos no controle autonômico não só no repouso, mas também durante o EF, sendo o receptor α2A-adrenérgico o responsável pela hiperatividade simp

  10. Empowering student leaders to nurture the first year experience through cross-cultural diversity. A Practice Report Nāu te raurau, Nāku te raurau, ka ora ai te iwi

    Directory of Open Access Journals (Sweden)

    Deborah Laurs

    2013-08-01

    Full Text Available Victoria University offers a number of peer-support and mentoring programs for first-year students, including Te Pūtahi Atawahi (mentoring and holistic support for Māori and Pasifika students, PASS (Peer Assisted Study Support and Campus Coaches (Orientation Week guides. In the past, despite having similar goals of providing peer-support to foster a sense of community, such programs have operated in isolation. However, a recent initiative has seen the development of a core leadership training module, jointly designed and delivered by staff from Te Pūtahi Atawhai and Student Learning Support. Seeking to equip student-leaders with an understanding of some of the holistic Māori values, Kotahitanga (unity, Ako (teaching and learning, Manaakitanga (empathy/hospitality, Whakamana (respect, Whakanui (acknowledging success, Whakawhanaungatanga (building strong networks and Rangatiratanga (ability to bring groups together/Self-direction, this core training offers the potential to strengthen Treaty of Waitangi relationships and nurture inter-cultural awareness, developing a pan-university sense of belongingness in the process. 

  11. Výroba dětské stoličky pro stomatologickou ordinaci s využitím metody Fused Deposition Modeling

    OpenAIRE

    Kyseľová, Barbora

    2013-01-01

    Bakalárska práca popisuje tvorbu návrhu modelu detskej stoličky v tvare ľudského zuba využiteľnou v stomatologickej ordinácii. Práca je radená do nadväzujúcich oddielov tak, ako bolo pri návrhu postupované. V úvode práce je venovaná pozornosť vytvoreniu fyzického modelu, ďalej predostrie využitie ručného 3D skenera a v rámci digitalizácie následne podloží nevyhnutnú úpravu dát získanú po skenovaní. Práca sa tiež zaoberá výrobou modelu stoličky, ktorá je realizovaná na 3D tlačiarni uPrint s vy...

  12. Sergej Dovlatov: Kofer /S ruskog preveo Petar Karavlah

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    Petar Karavlah

    2012-12-01

    Full Text Available Kuja iz Ureda za vize rekla je: „Svaki emigrant ima pravo na tri kofera. Takva je norma. To su posebni propisi ministarstva.“Nije imalo smisla proturječiti. Ali ipak sam morao: „Samo tri kofera?! Što bih trebao sa stvarima?“„Na primjer?“„Na primjer, sa svojom zbirkom trkaćih auta?“„Prodajte je“, odsutno odgovori službenica. Zatim doda, lagano se namrštivši: „Ako nečim niste zadovoljni, podnesite žalbu.“„Zadovoljan sam.“Nakon zatvora bio sam svime zadovoljan.„Pa onda se i ponašajte tako.“Za tjedan dana već sam pakirao stvari. Ispostavilo se da mi je bio dovoljan jedan jedini kofer. Gotovo sam zaplakao od muke. Pa imao sam trideset i šest godina. Od toga sam osamnaest godina radio. Zaradio bih pa kupio nešto. Mislio sam da imam nekakvu imovinu. A na kraju – jedan kofer. I to prilično skromnih dimenzija. Ispada da sam siromah? Kako je do toga došlo?Knjige? Imao sam uglavnom zabranjene knjige. One nisu prolazile kroz carinu. Morao sam ih podijeliti poznanicima zajedno s takozvanim arhivom.

  13. Mikroprogram pre riadenie procesu spájania operandov v architektúre DF KPI

    OpenAIRE

    Norbert Ádám

    2013-01-01

    V článku uvedená architektúra je založená na výpočtovom modeli „data flow“, v ktorom riadenie výpočtu je založené na spracovaní toku dát. Základným prvkom DF architektúry je koordinačný procesor (CP), ktorý je zodpovedný za riadenie a organizáciu vykonávania inštrukcií. Štruktúrna organizácia CP je navrhnutá ako dynamický multifunkčný systém. Pri vykonávaní operácií môže CP prechádzať rôznymi stavmi, v dôsledku čoho táto jednotka predstavuje dynamický prúdový systém. Prechod a poradie prechod...

  14. Analýza sendvičových panelů zatížených tlakem metodou konečných prvků

    OpenAIRE

    Seman, Matúš

    2016-01-01

    Bakalárska práca sa venuje možnostiam výpočtového modelovania porúch vznikajúcich v sendvičových paneloch pod tlakovým zaťažením s využitím metódy konečných prvkov. Úvodná, teoretická, časť je zameraná na požiadavky kladené na sendvičové konštrukcie, ako aj matematickú formuláciu konštitutívnych vzťahov. Cieľom praktickej časti bolo stanoviť vhodné spôsoby formulácie problému straty stability s aplikáciou na konkrétne laboratórne vzorky. Analýza bola vykonaná v nelineárnych riešičoch programo...

  15. Nekonvenční koncepce spalovacích motorů

    OpenAIRE

    Šenkýř, Miroslav

    2011-01-01

    Práca je zameraná na nekonvenčné koncepcie spaľovacích motorov, teda na spaľovacie motory neobvyklých konštrukcií. Sú to motory, ktoré majú odlišné tvary spaľovacieho priestoru tvary hriadeľov a merných výkonov od konvenčných koncepcii. Práca opisuje nekonvenčné motory ako je Wankel a Nutating motor. Práca objasňuje konštrukciu, princíp činnosti výhody a nevýhody. Vzhľadom k stálemu zdokonaľovaniu konštrukcií týchto motorov majú perspektívu do budúcnosti aj v leteckom priemysle. The work i...

  16. Modelling height to diameter ratio – an opportunity to increase Norway spruce stand stability in the Western Carpathians

    Directory of Open Access Journals (Sweden)

    Bošeľa Michal

    2014-06-01

    Full Text Available Smrek je jednou z najrozšírenejších a ekonomicky najdôležitejších ihličnatých drevín v Európe. Taktiež je drevinou veľmi často poškodzovanou vetrovými kalamitami. Štíhlostný kvocient (pomer výšky a hrúbky stromu sa považuje za vhodný indikátor statickej stability jednotlivých stromov a porastov. Preto cieľom práce bola identifikácia hlavných faktorov, ktoré majú významný vplyv na hodnoty kvocientu. Jednoduchý model bol vytvorený ako pomôcka pre lesníka pri rozhodovaní o intenzite prebierok v smrekových porastoch s cieľom potenciálneho zvýšenia statickej stability a zachovania optimálnej produkcie. Využili sa údaje Národnej inventarizácie a monitoringu lesov SR (260 plôch a taktiež údaje z predchádzajúcich výskumných projektov (48 plôch tak aby reprezentovali čo najširšie rozpätie ekologických podmienok, v ktorých smrek v súčasnosti rastie. Výsledky ukázali, že najvýznamnejšími ekologickými faktormi boli: nadmorská výška a bonita stanovišťa. Taktiež niektoré porastové charakteristiky sa významne podieľali na variabilite kvocientu: podiel koruny z výšky stromu, hustota porastu a stredná porastová výška reprezentujúca rastovú fázu porastu. Výsledky ďalej preukázali, že ekologické podmienky sú významnejšie ako porastové charakteristiky, a preto tieto treba brať do úvahy pri rozhodovacom procese. To znamená, že ak sa porast nachádza na pre smrek veľmi bonitnom stanovišti, sú potrebné silnejšie prebierky od mladých rastových fáz, aby sa ovplyvňovaním hrúbkového prírastku vyrovnal vplyv rýchleho výškového rastu. Zároveň je dôležitá rozdielna intenzita výškového a hrúbkového rastu počas vývoja porastu

  17. Selected Issues of Legislation with regard to Operating Pharmacies in the Slovak Republic / Vybrané problémy právnej úpravy prevádzkovania lekární v podmienkach Slovenskej republiky

    Directory of Open Access Journals (Sweden)

    Capandová Petra

    2015-06-01

    Full Text Available Medzi základné práva a slobody, upravené v druhej hlave Ústavy Slovenskej republiky patrí aj právo na ochranu zdravia, bezplatnú zdravotnú starostlivosť na základe zdravotného poistenia, právo na zdravotnícke pomôcky za zákonom stanovených podmienok. Tieto práva sú garantované v ustanovení Čl. 40 Ústavy Slovenskej republiky v rámci ekonomických, sociálnych a kultúrnych práv a vzťahujú sa na každú fyzickú osobu, t. j. na človeka. Keďže sú tieto práva považované za ľudské práva v zmysle medzinárodného práva, sú garantované aj v mnohostranných medzinárodných zmluvách, ktorých signatárom je aj Slovenská republika, ako napríklad Medzinárodný pakt o hospodárskych, sociálnych a kultúrnych právach. Právna úprava prevádzkovania lekární v Slovenskej republike zahŕňa široké spektrum otázok a je pomerne rozsiahla. Snahou zákonodarcu bolo nastaviť také pravidlá, ktoré by stransparentnili a zjednodušili právne prostredie v tomto sektore. V niektorých oblastiach sú pravidlá dané legislatívou Európskej únie a slovenský právny poriadok ich iba prevzal. Najdôležitejším prvokom v tejto sfére, ktorý nie je priamo upravovaný legislatívou EÚ, je poskytovanie lekárenskej starostlivosti a využívanie systému verejného zdravotného poistenia v tejto oblasti. Hlavným cieľom príspevku je poukázať na vybrané problémy, ktoré sa spájajú s postavením prevádzkovateľa lekárne a jeho povinnosťami, ktoré mu vyplývajú zo zákona o liekoch a s ktorými sa musí ako prevádzkovateľ lekárne vysporiadať. Táto oblasť podnikania je pomerne prísne regulovaná a preto je potrebné byť oboznámený s príslušnými právnymi predpismi. Príspevok z právno-teoretického hľadiska rieši viaceré otázky upravené v zákone o liekoch, či vyhláške o správnej lekárenskej praxi.

  18. Dora Maček: Nijanse modrog u pjesmama Susanne Jorn

    Directory of Open Access Journals (Sweden)

    Susanne Jorn

    2012-06-01

    Full Text Available Pjesništvo Susanne Jorn intimno je s mnogo kratkih pjesama koje često izražavaju osjećaje, atmosferu i dojmove. Tu su osobna viđenja prirode i ljudi, doživljena, mogli bismo reći, na slikarski način. Boje su gotovo redoviti sastavni dio tih doživljaja i kao na slikama, stvaraju dramatičnu ili smirenu atmosferu. One su izraz prirode koja okružuje pjesnikinju, ali su i izraz osjećaja i duševnih stanja. To se jasno pokazuje u zbirci Ne sada već sad, čiji je okvir paleta plavih boja. Druga scena njezine poezije sličice su iz japanskog života i japanske umjetnosti i pjesništva, koje mnogo puta prenosi u svoju dansku okolinu. U zbirci Ne sada već sad mnoge su pjesme prizori iz Danske ili Tokija, ali i drugih krajeva svijeta gdje je boravila. Ipak, njezino je pjesništvo prije svega vezano uz nordijsko nebo, zemlju i kulturu te sjećanja na oca (poznatog danskog likovnog umjetnika Asgera Jorna, koji je u svojim djelima spajao različite vrste umjetnosti. Asger Jorn bio je član poznate transnacionalne udruge CoBrA, koja je djelovala početkom 1950-ih godina. U mnoge se pjesme određenih osjećaja i ozračja skladno uklapaju i japanski i danski krajolici te japanski i, ako tako možemo reći, nordijski pjesnički izraz. Unatoč vrlo intimnoj poeziji, kao i većina skandinavskih pjesnika i pjesnikinja, Susanne Jorn osjetljiva je na sudbu današnjeg čovjeka bilo gdje u svijetu i ona nalazi značajno mjesto u njezinu pjesništvu.

  19. Chuck Palahniuk: Negdje mora puknuti / S engleskog prevela Valentina Lisak

    Directory of Open Access Journals (Sweden)

    Chuck Palahniuk

    2011-12-01

    Full Text Available Iza zaključanih vrata zahoda u stražnjem dijelu antikvarnice Claire Upton razgovara na telefon. Glas joj odjekuje od poda i zidova: Koliko je teško provaliti nadzornu kameru? Ukrasti snimku? upita svog muža i počne plakati.U zadnjih tjedan dana bila je u tom dućanu već tri ili četiri puta. Jedan od onih gdje moraš ostaviti torbu na blagajni, inače te ne puste unutra. Moraš ostaviti i kaput, ako ima duboke, prostrane džepove. I kišobran, jer bi ljudi u nabore mogli ubacivati sitne predmete, češljeve, nakit i druge drangulije. Kraj starog blagajnika stoji sivi karton na kojem crnim markerom piše: “Ne volimo kad nas kradete!”Skidajući kaput, Claire reče: “Ja nisam lopov.”Stari blagajnik pogledom ju je odmjerio od glave do pete. Coknuo je jezikom i pitao: “Po čemu ste vi iznimka?”Za svaki ostavljeni predmet dao je Claire polovicu karte. Za torbicu hercova asa. Za kaput trefovu devetku. Za kišobran pikovu trojku.Pogledom je prešao preko Claireinih ruku, obrisa džepova na prsima i najlonki tražeći izbočine, nešto ukradeno. Iza prednjeg pulta po cijeloj su trgovini visjeli mali natpisi koji su upozoravali da je ovdje zabranjeno krasti. Kamere su nadzirale svaki prolaz, svaki kutak i sve prikazivale na malenom ekranu punom drugih ekrana. Trezor malih, crno-bijelih tv-ekrana iza blagajne gdje je sjedio starac i sve ih promatrao.

  20. Ugovor o zajedničkom pothvatu (joint venture u naftnom sektoru

    Directory of Open Access Journals (Sweden)

    Ratko Brnabić

    2014-10-01

    Full Text Available U radu se raspravlja o ugovoru o zajedničkom pothvatu koji je praktički neizostavan dio svake ozbiljnije operacije istraživanja, a potom i iskorištavanja ugljikovodika (proizvodnja naftnih derivata. Riječ je o skupom pothvatu uz koji se vezuju brojni rizici pa trgovci u tom području žele sklapanjem takvog ugovora zadržati mogućnost (izglede da ostvare dobit, ali pritom podijele rizik. U prvom dijelu rada daje se prikaz bitnih značajki zajedničkog pothvata s posebnim osvrtom na područje iskorištavanja ugljikovodika. S obzirom na okolnost da se za potrebe zajedničkog pothvata često osniva trgovačko društvo kapitala, raspravlja se o pravnim odnosima članova tog društva međusobno te prema upravi društva. Skreće se pozornost i na razlike u značenju pojma joint venture posebice u američkom i hrvatskom pravu što može izazvati ozbiljne pravne učinke ako ortaci nemaju sjedište u istoj državi. Nakon toga, obrađuju se bitna pitanja koja ugovorne strane neizostavno moraju urediti da bi se izbjegli sudski sporovi. To su, primjerice, pitanje područja istraživanja, pravni položaj operatora, sredstva osiguranja, standard kvalitete pri obavljanju poslova, pravo na informiranje i nadzor te pitanje sustava rješavanja sukoba u ortaštvu. U zaključku se iznose rezultati istraživanja te se upućuje na moguća rješenja za otvorena pitanja uređenja međusobnog odnosa ortaka u zajedničkom pothvatu.

  1. Energetski izazovi: europska gledišta i francuski odgovori

    Directory of Open Access Journals (Sweden)

    Bosiljka Britvić Vetma

    2013-11-01

    Full Text Available Europska unija nastoji poboljšati europsko energetsko tržište na način da bude bolje koordinirano, jer u ujedinjenoj Europi nije više moguće djelovati izdvojeno. Proizvodnja energije iz obnovljivih izvora velikih razmjera zahtijeva međusobno povezano europsko energetsko tržište, ako se žele postići ciljevi utvrđeni programom „Energija i klima“ usvojenim 2009. Cilj je ovog rada utvrditi na koji način utječu pravila europskog prava na pravno normiranje u energetskim pitanjima i borbi protiv globalnog zatopljenja. Razvoj energija koje ne emitiraju ugljični dioksid u skladu je s ciljevima koje postavlja Europska unija, koji se u tom području oslanja na regije, a ne više na same države koje nisu uvijek u mogućnosti provesti definirane ambiciozne ciljeve. Energija nastavlja ovisiti u većini slučajeva o snazi država i europska nadležnost je ograničena. Europska unija sudjeluje uglavnom u koordiniranju i podržavanju nacionalnih politika, u okviru odredaba koje se odnose na transeuropske mreže i u okviru politike zaštite okoliša. U načelu, mjere koje se odnose na izbor svake države između različitih izvora energije i na opću strukturu njene opskrbe energijom jednoglasno su usvojene. Posebna pozornost posvećena je uspoređivanju francuskih pravnih pravila koja se primjenjuju u razvoju obnovljivih izvora energije, prilagodbi obnovljivim izvorima energije prava o zaštiti okoliša i urbanističkog planiranja i važnosti nadzora, s europskim pravnim standardima reguliranja.

  2. Pleiotropic effects of methionine adenosyltransferases deregulation as determinants of liver cancer progression and prognosis.

    Science.gov (United States)

    Frau, Maddalena; Feo, Francesco; Pascale, Rosa M

    2013-10-01

    Downregulation of liver-specific MAT1A gene, encoding S-adenosylmethionine (SAM) synthesizing isozymes MATI/III, and upregulation of widely expressed MAT2A, encoding MATII isozyme, known as MAT1A:MAT2A switch, occurs in hepatocellular carcinoma (HCC). Being inhibited by its reaction product, MATII isoform upregulation cannot compensate for MATI/III decrease. Therefore, MAT1A:MAT2A switch contributes to decrease in SAM level in rodent and human hepatocarcinogenesis. SAM administration to carcinogen-treated rats prevents hepatocarcinogenesis, whereas MAT1A-KO mice, characterized by chronic SAM deficiency, exhibit macrovesicular steatosis, mononuclear cell infiltration in periportal areas, and HCC development. This review focuses upon the pleiotropic changes, induced by MAT1A/MAT2A switch, associated with HCC development. Epigenetic control of MATs expression occurs at transcriptional and post-transcriptional levels. In HCC cells, MAT1A/MAT2A switch is associated with global DNA hypomethylation, decrease in DNA repair, genomic instability, and signaling deregulation including c-MYC overexpression, rise in polyamine synthesis, upregulation of RAS/ERK, IKK/NF-kB, PI3K/AKT, and LKB1/AMPK axis. Furthermore, decrease in MAT1A expression and SAM levels results in increased HCC cell proliferation, cell survival, and microvascularization. All of these changes are reversed by SAM treatment in vivo or forced MAT1A overexpression or MAT2A inhibition in cultured HCC cells. In human HCC, MAT1A:MAT2A and MATI/III:MATII ratios correlate negatively with cell proliferation and genomic instability, and positively with apoptosis and global DNA methylation. This suggests that SAM decrease and MATs deregulation represent potential therapeutic targets for HCC. Finally, MATI/III:MATII ratio strongly predicts patients' survival length suggesting that MAT1A:MAT2A expression ratio is a putative prognostic marker for human HCC.

  3. The Evaluation of Selected Air Pollutants in Košice for the Period 2008-2010 / Analýza Znečisťujúcich Látok Vo Vybraných Monitorovacích Staniciach Mesta Košice Za Obdobie 2008 - 2010

    Directory of Open Access Journals (Sweden)

    Gergeľová Marcela

    2014-03-01

    Full Text Available Vo všeobecnosti kvalita ovzdušia je stanovená z obsahu znečisťujúcich látok vo vonkajšom ovzduší. Kritériá kvality ovzdušia (limitné a cieľové hodnoty, medze tolerancie, horné a dolné medze na hodnotenie vychádzajú z platného legislatívneho rámca. V podmienkach SR kritéria kvality ovzdušia vyplývajú z vyhlášky MŽP SR č. 360/2010 Z.z. o kvalite ovzdušia [1]. V nadväznosti na realizované merania je potrebné zvoliť efektívne nástroje potrebné pre preprocesing a postprocesing procesu plošného hodnotenia kvality ovzdušia. Príspevok si kladie za cieľ aplikovať vhodné nástroje GIS do hodnotiaceho procesu kvality ovzdušia v aglomerácií Košice za vybrané obdobie rokov 2008 - 2010. Pri celkovom zhodnotení predmetnej oblasti bolo uvažované so spracovaním informácií o emisiách deklarovaných ako tuhé znečisťujúce látky (TZL v zmysle konzervatívneho prístupu k hodnoteniu kvality ovzdušia za PM10. Pre posudzovanie boli použité ročenky a správy pre kvalitu ovzdušia v SR za rok 2008 - 2010 z SHMÚ a odborné publikácie

  4. REFORMA EKONOMIJE

    Directory of Open Access Journals (Sweden)

    Joseph Stiglitz

    2013-12-01

    Full Text Available Dosta je krivice koja se deli u ovoj krizi. Videli smo ulogu regulatornih tela i zakonodavaca, FED-a i finansijera, svih njih koji su bili u igri. Svako od njih je govorio o svom interesu zauzimajući stav da su bili u pravu za ono što su radili a veoma često zasnivajući svoje stavove na ekonomskoj analizi. Kada počne raslojavanje pitanja šta je pošlo naopako ne može se izostaviti fokus sa ekonomske profesije. Naravno, nisu se svi ekonomisti pridružili slavi slobodnog ekonomskog tržišta i nisu svi bili sledbenici Miltona Fridmana. Iznenađujuće je što je ipak, veliki deo bio naklonjen tom pravcu. Ne samo da je njihov savet bio pogrešan već su bile pogrešne i osnovne pretpostavke i predviđanja. Relativno mali broj njih je mogao da vidi nadolazeću katastrofu. Nije bilo slučajno da su oni, koji su se zalagali za pravila koja su dovela do nesreće, bili toliko zaslepljeni svojom verom u slobodno tržište te nisu mogli da vide probleme koji nastaju. Ekonomija se promenila više nego što su to ekonomisti želeli da veruju, do te mere da je od naučne discipline postala navijačica discipline slobodnog tržišta. Ako SAD želi da uspe u reformi privrede, moraće da počne sa reformom ekonomije.

  5. »Kommunikation und Kybernetik in Einzeldarstellungen”. Herausgegeben von H. Wolter und W. D. Keidel. Band 2: Structural Linguistics and Human Communication. An Introduction into the Mechanism of Language and the Methodology of Linguistics By Bertil Malmbe

    Directory of Open Access Journals (Sweden)

    Žarko Muljačić

    2016-02-01

    Full Text Available Za razliku od priručnika istog pisca New Trends in Linguistics. An Orientation, Stockholm-Lund 1964 (koji predstavlja dotjerani i ažurirani prijevod švedskog originala iz 1959. god. i o kome v. recenziju koju smo objavili u »ZFLMS« X, Novi Sad 1967, str. 181-188, ova je odlična monografija namijenjena uzem krugu čitalaca koji već imaju dovoljnih znanja iz lingvistike i fonetike ili iz društvenih i prirodnih nauka, kojima su od koristi tekovine i metode suvremene lingvistike (psihologija, fonijatrija, logoterapija, metodika učenja stranih jezika, nauka o komunikacijama i sl.. Autor, ugledni romanist i profesor fonetike na sveučilištu u Lundu, a od nedavna i na Sorbonni, svijestan je opasnosti da će lingvistika, prikazana prvenstveno kao znanost koja studira jezično komuniciranje ljudi, nužno morati da se pozabavi i vrlo širokim krugom problema pa će tako neka poglavlja ispasti za jedne čitaoce prekomplicirapa a za druge preelementarna. Drugačije međutim nije moglo biti ako se željelo na relativno malenom prostoru prikazati tako različite pojmove kao sto su fonemi i značenje, decibeli i jezicna promjena, knjizevni stil i govorne smetnje. Metodski pristup gradi i kompozicija sasvim su drugačiji nego u New Trends in Linguistics. Dok su tamo poglavlja posvečena pojedinim lingvističkim školama i pravcima (a samo indirektno i nekim skupinama problema ukoliko se neka škola uglavnom njiina bavila, ova je monografija obrađena problemski.

  6. IDENTITET I UČEŠĆE UČENICA MUSLIMANKI U NASTAVI FIZIČKOG VASPITANJA U ENGLESKOJ

    Directory of Open Access Journals (Sweden)

    Ken Hardman

    2012-06-01

    Full Text Available Ovaj članak se oslanja na etnografsku studiju slučaja grupe učenica muslimanki u dvije škole u Engleskoj. Istraživanje razmatra pitanja oko njihovog vjerskog i etničkog identiteta i dali je to u suprotnosti s njihovi učestvovanjem u nastavi fizičkog vaspitanja (FV. Teorija socijalnog identiteta na koju se oslanja ovo istraživanje, fokusira se na istraživanje i interpretaciju empirijskih podataka prikupljenih u razdoblju od dvadeset mjeseci, uglavnom detaljnih polu-strukturisanih intervjua za tumačenje iskustva FV uz korištenje kvalitativne metodologije.Socijalne kategorije etničke pripadnosti i vjeroispovijesti igraju ključnu ulogu u formiranju identiteta učenica muslimanki. Djevojke doživljavaju FV kao predmet koji dopušta slobode kojih nema nigdje drugdje u nastavnom planu i programu i one prepoznaju važnost fizičke aktivnosti. Studija potvrđuje rezultate prethodnih istraživanja koja su utvrdila da pitanja opreme u postu tokom Ramazana i vannastavne aktivnosti predstavljaju problem za učenike muslimane.To su stvari koje se posebno usložavaju kada nastavnici nisu svjesni ovog problema.Rezultati su pokazali potrebu odstupanja od postavki tradicionalnog fizičkog vaspitanja. Iskustva učenika su se više oslanjaju na kvalitet pojedinih nastavnika. Izgleda da su kursevi multikulturalnosti i svijeti o rasizmu neophodni za bolje razumijevanje učenika i mogu da budu od pomoći ako ih učinimo dostupnim svim učiteljima, bez obzira na njihov hijerarhijski položaj.

  7. A C-type lectin receptor pathway is responsible for the pathogenesis of acute cyclophosphamide-induced cystitis in mice.

    Science.gov (United States)

    Dejima, Takashi; Shibata, Kensuke; Yamada, Hisakata; Takeuchi, Ario; Hara, Hiromitsu; Eto, Masatoshi; Naito, Seiji; Yoshikai, Yasunobu

    2013-12-01

    Hemorrhagic cystitis often arises after cyclophosphamide (CYP) administration. As yet, however, the mechanism involved in its pathogenesis is unknown. In this study, it was found that the Fc receptor γ chain (FcRγ)- caspase recruitment domain-containing protein 9 (CARD9)-dependent pathway rather than the myeloid differentiation primary response gene 88 (MyD88)-dependent pathway is involved in the pathogenesis of acute CYP-induced cystitis in mice. Rapid and transient production of interleukin (IL)-6 and IL-1β was detected in the bladder at 4 hr, preceding IL-23 and IL-17A production and an influx of neutrophils, which reached a peak at 24 hr after injection. As assessed by weight, edema and neutrophil infiltration, cystitis was significantly attenuated in CARD9 knockout (KO) and FcRγKO mice, this attenuation being accompanied by impaired production of IL-1β, IL-6, IL-23 and IL-17A. The major source of IL-17A is the vesical γδ T cell population: IL-17AKO, CδKO and Tyk2KO mice showed little IL-17A production and reduced neutrophil infiltration in the bladder after CYP injection. These results suggest that FcRγ-CARD9-dependent production of proinflammatory cytokines such as IL-1β, IL-6, and IL-23 and the subsequent activation of IL-17A-producing γδ T cells are at least partly involved in the pathogenesis of acute CYP-induced cystitis in mice. PMID:24102807

  8. Above-ground net primary productivity in young stands of beech and spruce

    Directory of Open Access Journals (Sweden)

    Pajtík Jozef

    2013-09-01

    Full Text Available V tejto práci sme pomocou kombinácií kontinuálnych meraní a deštruktívnych odberov vzorníkov sledovali a porovnali zásobu nadzemnej biomasy a ročnú primárnu produkciu (NPP v prirodzene obnovených mladých porastoch buka a smreka. Na vybranej lokalite predpokladáme, že zmenené klimatické podmienky budú lepšie vyhovovať buku pred v súčasnosti prevládajúcim smrekom. Lokalita Vrchslatina sa nachádza v južnej časti Veporských vrchov v nadmorskej výške 977 m nad morom (48° 38΄ 50΄΄ N, 19° 36΄ 07΄΄ E. Priemerné ročné zrážky sa pohybujú okolo 900 mm, priemerná ročná teplota je 5,2 °C. Na sledovanej lokalite sme pozorovali odlišný priebeh rastu buka a smreka. Pri buku bola pozorovaná menšia medziročná mortalita, udržiaval sa až prehustený zápoj, v ktorom sa aj podúrovňové stromy snažili dostať do úrovne. Toto sa prejavilo na tvare kmeňov, ktoré sú tenké a vysoké. Štíhlostný koeficient sa pri stromoch so strednou hrúbkou a strednou výškou postupne zvyšoval od 1,19 do 1,40. Pri smreku dochádza k vyššej mortalite, vrastavé a podúrovňové stromy odumierajú z dôvodu nedostatku svetla. Stromy rastú viac do hrúbky, čo sa odráža aj na štíhlostnom koeficiente, ktorý bol po celé obdobie viac-menej konštantný a pohyboval sa v rozpätí 0,89 až 0,93 (tab. 1. Zásoby kmeňa sú pri smreku v jednotlivých rokoch o 3 - 10 m3.ha-1 väčšie ako pri buku (tab. 3. Po prepočítaní na sušinu je vplyvom rozdielnej objemovej hmotnosti (obr. 5 celková zásoba sušiny drevných častí (kmeň a konáre väčšia pri buku (tab. 3. Najväčší rozdiel medzi drevinami je v zásobe asimilačných orgánov, ktorá je pri smreku viac než trojnásobná (tab. 3 a 4. Počas rastu dochádza pri obidvoch drevinách k zvyšovaniu podielu kmeňa a znižovaniu podielu asimilačných orgánov (obr. 2. Hlavný medzidruhový rozdiel pri pomerne vyrovnaných hektárových zásobách je v

  9. Carolus van Europa. Keizer Karel V in 2000 herdacht

    Directory of Open Access Journals (Sweden)

    M.E.H.N. Mout

    2001-01-01

    Full Text Available Th. Coppens, Antonius Mor. Hofschilder van Karel V R. de Dijn, De vrouwen van de keizer. Een tocht door Europa met Karel V A. Kohler, Karl V. 1500-1558. Eine Biographie H. Verbrugge, Keizer Karel. Testament van een Habsburger H. Lox, Van stropdragers en de pot van Olen. Verhalen over Keizer Karel E. Schreurs, De schatkamer van Alamire. Muziek en miniaturen uit keizer Karels tijd (1500-1535 E. Schulin, Kaiser Karl V. Geschichte eines übergroßen Wirkungsbereiches M.F. Alvarez, Carlos V, el césar y el hombre P.N. Palacio, Carolus V imperator F. Maes, The empire resounds. Music in the days of Charles V H. Soly, J. van de Wiele, Carolus. Keizer Karel V 1500-1558 R. Hoozee, J. Tollebeek, T. Verschaffel, Mise-en-scène. Keizer Karel en de verbeelding van de negentiende eeuw H. Soly, Karel V, 1500-1558. De keizer en zijn tijd S. MacDonald, Charles V. Ruler, dynast and defender of the faith 1500-58 W. Blockmans, Keizer Karel V 1500-1558. De utopie van het keizerschap G. Vanpaemel, T. Padmos, Wereldwijs. Wetenschappers rond keizer Karel T. Padmos, G. Vanpaemel, De geleerde wereld van keizer Karel. Catalogus tentoonstelling Wereldwijs. Wetenschappers rond keizer Karel W. Blockmans, Het keizerschap van Karel V. Europese droom versus regionale werklijkheidCarolus of Europe. The Emperor Charles V commemorated in the year 2000The commemoration of 1500, the year of Charles's birth, has led to many and multifarious publications. In some of them a not unattractive mixture of scholarly research and literary imagination prevails. The big exhibitions evoke an image of the Emperor placed on the intersection between political power and cultural flourishing. Specialized studies examine themes from musicology and the history of science. Biographies vary between modest introductions and bulky volumes full of detail. A few research desiderata, especially in the field of economic and financial history, are still unfulfilled. In every book reviewed the European

  10. Advances of DNA Sequencing Technology and Its Applications%DNA测序技术及其应用研究进展

    Institute of Scientific and Technical Information of China (English)

    刘朋虎; 林冬梅; 林占熺; 李晶

    2012-01-01

    In this paper, we introduced principles and characteristics of the first, second and third generation of sequencing technology, then the applications of second sequencing technology were described. Because of complicated operation and high cost, the first generation DNA sequencing technology represented by Sanger sequencing method can not meet the needs of large - scale sequencing. The second generation DNA sequencing technology characterized by high-throughout and low cost including Solexa sequencing technology of Illumina, and Applied Biosystems SOLiD and Roche 454 now has been used in many fields of life science research. The third-generation sequencing technology which can sequence single DNA molecular has also been arisen, but not been widely used in life science research Key words .%本文首先介绍了第一代、第二代、第三代DNA测序技术的原理、特点,在此基础上介绍了第二代测序技术在基因组测序、重测序,RNA测序,宏基因组,DNA甲基化等方面的应用.第一代测序技术以Sanger测序法为代表,操作繁琐、成本较高,不能满足大规模测序的需要.第二代测序技术以高通量、低成本为主要特点,主要包括Illumina公司的Solexa测序技术、罗氏公司的454测序技术和ABI公司的SOLiD测序技术,目前已广泛应用于生命科学研究的各个领域.第三代测序技术以单分子测序为主要特点,目前已经初见端倪,但是还没有被大规模广泛应用.

  11. The Use and Abuse of Jus Nexi

    Directory of Open Access Journals (Sweden)

    Noah Benjamin Novogrodsky

    2012-09-01

    Full Text Available This paper uses Shachar’s conception of jus nexi to explore three interrelated ideas. I first contend that Shachar’s analysis of the monetary value of birthright citizenship may be applied to temporary workers, lawful permanent residents and naturalized citizens as an exposé of inherited privilege in diverse communities and as a means of identifying which forms of membership and belonging are worth owning. Second, I use the idea of jus nexi to question which additional work relationships and identity networks that might qualify as genuine connections to a given state. Finally, I question whether an operationalized version of jus nexi, that is an alternative category of citizenship, would supplant or complement existing jus soli and jus sanguinis rules. Here, I seek to apply Shachar’s theoretical contributions to current political debates and warn that a genuine connection test is increasingly being misused to support a nativist agenda.Cet article fait appel à la conception du jus nexi de Shachar pour explorer trois idées inter-reliées. Premièrement, nous soutenons que l’analyse de la valeur monétaire de la citoyenneté par droit de naissance de Shachar peut être appliquée aux travailleurs temporaires, aux résidents permanents qui ont un statut légal et aux citoyens naturalisés en tant qu’exposé des privilèges hérités dans des communautés différentes et comme un moyen d’identifier les formes d’appartenance et d’être ensemble qu’il vaut la peine de posséder. Deuxièmement, nous faisons appel à l’idée de jus nexi pour questionner les relations de travail additionnelles et les réseaux identitaires qui pourraient être reconnues en tant que liens authentiques à un État donné. Enfin, nous nous demandons si une version opérationnelle du jus nexi, entendons une catégorie alternative de citoyenneté, complèterait ou remplacerait les règles existantes du jus soli et du jus sanguinis. Ici, nous essayons d

  12. In vivo translocation and toxicity of multi-walled carbon nanotubes are regulated by microRNAs

    Science.gov (United States)

    Zhao, Yunli; Wu, Qiuli; Li, Yiping; Nouara, Abdelli; Jia, Ruhan; Wang, Dayong

    2014-03-01

    We employed an in vivo Caenorhabditis elegans assay system to perform SOLiD sequencing analysis to identify the possible microRNA (miRNA) targets of multi-walled carbon nanotubes (MWCNTs). Bioinformatics analysis on targeted genes for the identified dysregulated miRNAs in MWCNT exposed nematodes demonstrates their involvement in many aspects of biological processes. We used loss-of-function mutants for the identified dysregulated miRNAs to perform toxicity assessment by evaluating functions of primary and secondary targeted organs, and found the miRNA mutants with susceptible or resistant property towards MWCNT toxicity. Both the physiological state of the intestine and defecation behavior were involved in the control of the susceptible or resistant property occurrence for specific miRNA mutants towards MWCNT toxicity. This work provides the molecular basis at the miRNA level for future chemical design to reduce the nanotoxicity of MWCNTs and further elucidation of the related toxicological mechanism.We employed an in vivo Caenorhabditis elegans assay system to perform SOLiD sequencing analysis to identify the possible microRNA (miRNA) targets of multi-walled carbon nanotubes (MWCNTs). Bioinformatics analysis on targeted genes for the identified dysregulated miRNAs in MWCNT exposed nematodes demonstrates their involvement in many aspects of biological processes. We used loss-of-function mutants for the identified dysregulated miRNAs to perform toxicity assessment by evaluating functions of primary and secondary targeted organs, and found the miRNA mutants with susceptible or resistant property towards MWCNT toxicity. Both the physiological state of the intestine and defecation behavior were involved in the control of the susceptible or resistant property occurrence for specific miRNA mutants towards MWCNT toxicity. This work provides the molecular basis at the miRNA level for future chemical design to reduce the nanotoxicity of MWCNTs and further elucidation of the

  13. Pixel Analysis and Plasma Dynamics Characterized by Photospheric Spectral Data

    Science.gov (United States)

    Rasca, Anthony P.; Chen, James; Pevtsov, Alexei A.

    2016-05-01

    Recent observations of the photosphere using high spatial and temporal resolutions show small dynamic features at the resolving limit during emerging flux events. However, line-of-sight (LOS) magnetogram pixels only contain the net uncanceled magnetic flux, which is expected to increase for fixed regions as resolution limits improve. A new pixel dynamics method uses spectrographic images to characterize photospheric absorption line profiles by variations in line displacement, width, asymmetry, and peakedness and is applied to quiet-sun regions, active regions with no eruption, and an active region with an ongoing eruption. Using Stokes I images from SOLIS/VSM on 2012 March 13, variations in line width and peakedness of Fe I 6301.5 Å are shown to have a strong spatial and temporal relationship with an M7.9 X-ray flare originating from NOAA 11429. This relationship is observed as a flattening in the line profile as the X-ray flare approaches peak intensity and was not present in area scans of a non-eruptive active region on 2011 April 14. These results are used to estimate dynamic plasma properties on sub-pixel scales and provide both spatial and temporal information of sub-pixel activity at the photosphere. The analysis can be extended to include the full Stokes parameters and study signatures of magnetic fields and coupled plasma properties.

  14. Application of Next Generation Sequencing Techniques in Food Microbiology%新一代测序技术在食品微生物学中的应用

    Institute of Scientific and Technical Information of China (English)

    韩齐; 李媛媛; 孙方达; 孔保华; 陈倩

    2016-01-01

    近年来,焦磷酸测序、SOLiD测序等一类新型测序技术引起了人们广泛的关注,这些技术统称为新一代测序(Next generation sequencing,NGS)技术.与第一代Sanger测序技术相比,NGS测序技术具有检测通量更高、用时更少、准确度更高以及检测费用更低等优点.序列的检测可以用于精确的鉴别微生物菌群,包括不可培养的生物体和含量极少的微生物,NGS技术的研发给微生物学研究带来了巨大的变革.通过对NGS系统和平台的介绍,综述了NGS技术在食品微生物学和食品发酵研究中的应用,并探讨了它们的局限性和发展前景,该技术的应用可以增加对微生物多样性、菌群结构和微生物数量动态变化的研究,从而提高对食品(主要是发酵食品)的监控.

  15. Comparison of Ground- and Space-based Longitudinal Magnetograms

    CERN Document Server

    Pietarila, A; Harvey, J W; Pevtsov, A A

    2012-01-01

    We compare photospheric line-of-sight magnetograms from the Synoptic Long-term Investigations of the Sun (SOLIS) vector spectromagnetograph (VSM) instrument with observations from the 150-foot Solar Tower at Mt. Wilson (MWO), Helioseismic and Magnetic Imager (HMI) on Solar Dynamics Observatory (SDO), and Michelson Doppler Imager (MDI) on Solar and Heliospheric Observatory (SOHO). We find very good agreement between VSM and the other data sources for both disk-averaged flux densities and pixel-by-pixel measurements. We show that the VSM mean flux density time series is of consistently high signal-to-noise with no significant zero-offsets. We discuss in detail some of the factors -spatial resolution, flux dependence and position on the solar disk- affecting the determination of scaling between VSM and SOHO/MDI or SDO/HMI magnetograms. The VSM flux densities agree well with spatially smoothed data from MDI and HMI, although the scaling factors show clear dependence on flux density. The factor to convert VSM to H...

  16. Maximum power analysis of photovoltaic module in Ramadi city

    Energy Technology Data Exchange (ETDEWEB)

    Shahatha Salim, Majid; Mohammed Najim, Jassim [College of Science, University of Anbar (Iraq); Mohammed Salih, Salih [Renewable Energy Research Center, University of Anbar (Iraq)

    2013-07-01

    Performance of photovoltaic (PV) module is greatly dependent on the solar irradiance, operating temperature, and shading. Solar irradiance can have a significant impact on power output of PV module and energy yield. In this paper, a maximum PV power which can be obtain in Ramadi city (100km west of Baghdad) is practically analyzed. The analysis is based on real irradiance values obtained as the first time by using Soly2 sun tracker device. Proper and adequate information on solar radiation and its components at a given location is very essential in the design of solar energy systems. The solar irradiance data in Ramadi city were analyzed based on the first three months of 2013. The solar irradiance data are measured on earth's surface in the campus area of Anbar University. Actual average data readings were taken from the data logger of sun tracker system, which sets to save the average readings for each two minutes and based on reading in each one second. The data are analyzed from January to the end of March-2013. Maximum daily readings and monthly average readings of solar irradiance have been analyzed to optimize the output of photovoltaic solar modules. The results show that the system sizing of PV can be reduced by 12.5% if a tracking system is used instead of fixed orientation of PV modules.

  17. What can co-ethnic immigrants tell us about ethnic visions of the national self? A comparative analysis of Germany and Greece

    Directory of Open Access Journals (Sweden)

    Christin Heß

    2011-01-01

    Full Text Available Nations with a predominantly ethno-cultural self-perception and citizenship based on jus sanguinis are under pressure today to adopt more civic-territorial ideas of nationhood, including elements of jus soli. Two nations experiencing these trends in Europe but have rarely been juxtaposed are Greece and Germany. Characteristic of both nations is a long reserved privileged access to citizenship and settlement assistance for co-ethnic immigrants from Eastern Europe and recently the Former Soviet Union. This article argues that changes to the way these privileged immigrant groups and their settlement are addressed should also reflect changes to the national idiom. The paper contrasts Greece to Germany and finds that, similarly to developments in its northern counterpart, Greek repatriates from the Former Soviet Union have been an important consequence of the ethno-cultural idiom and reinforced it at times. In the new millennium these immigrants’ importance is diminishing in reality, if not on paper. The article concludes that in spite of this and the citizenship reform of 2010, the tendency to see the country as a culturally homogeneous nation is still fairly strong in Greece. The analysis draws on interviews with ‘repatriates’ in both countries and with national policy-makers in Greece, as well as on newspaper clippings, opinion polls and statistical data, complemented by leading scholarship in the field to date.

  18. Wicket Fall Concept Mining From Cricket Video Using A-Priori Algorithm

    Directory of Open Access Journals (Sweden)

    Mahesh Goyani

    2011-02-01

    Full Text Available In this paper, we propose an algorithm to detect semantic concepts from cricket video. In our previous work, we have proposed key frame detection based approach for semantic event detection andclassification. The proposed scheme works in two parts. In first part a top-down event detection and classification is performed using hierarchical tree. In second part, higher level concept is identified by applying A-Priori algorithm. In part 1, key frames are identified based on Hue Histogram difference at level 1. At level 2, logo transitions classify the frames as real-time or replay. At level 3, we classify the real time frames as field view, pitch view or non field view based on thresholds like Dominant Soli PixelRation (DSPR and Dominant Grass Pixel Ration (DGPR. At level 4, we detect close up and crowd frames based upon edge detection. At level 5a, we classify the close up frames into player of team A,player of team B and umpire based upon skin colour and corresponding jersey colour. At level 5b, we classify the crowd frames into spectators, player’s gathering of team A or player’s gathering of team B. In part two, labels are associated with each frame event, which is used as input to A-Priori algorithm for concept mining. Results at the end of paper show the robustness of our approach.

  19. Transcriptome Survey of a Marine Food Fish: Asian Seabass (Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Natascha M. Thevasagayam

    2015-06-01

    Full Text Available The Asian seabass (or barramundi; Lates calcarifer is a marine teleost and a popular food fish in Southeast Asia and Australia. To date, comprehensive genome and transcriptome sequence information has not been available for this species in public repositories. Here, we report a comprehensive de novo transcriptome assembly of the Asian seabass. These data will be useful for the development of molecular tools for use in aquaculture of Asian seabass as well as a resource for genome annotation. The transcriptome was obtained from sequences generated from organs of multiple individuals using three different next-generation sequencing platforms (454-FLX Titanium, SOLiD 3+, and paired-end Illumina HiSeq 2000. The assembled transcriptome contains >80% of the expected protein-coding loci, with 58% of these represented by a predicted full-length cDNA sequence when compared to the available Nile tilapia RefSeq dataset. Detailed descriptions of the various steps involved in sequencing and assembling a transcriptome are provided to serve as a helpful guide for transcriptome projects involving de novo assembly of short sequence reads for non-model teleosts or any species of interest.

  20. Calcite-forming bacteria for compressive strength improvement in mortar.

    Science.gov (United States)

    Park, Sung-Jin; Park, Yu-Mi; Chun, Woo-Young; Kim, Wha-Jung; Ghim, Sa-Youl

    2010-04-01

    Microbiological calcium carbonate precipitation (MCP) has been investigated for its ability to improve the compressive strength of concrete mortar. However, very few studies have been conducted on the use of calcite-forming bacteria (CFB) to improve compressive strength. In this study, we discovered new bacterial genera that are capable of improving the compressive strength of concrete mortar. We isolated 4 CFB from 7 environmental concrete structures. Using sequence analysis of the 16S rRNA genes, the CFB could be partially identified as Sporosarcina soli KNUC401, Bacillus massiliensis KNUC402, Arthrobacter crystallopoietes KNUC403, and Lysinibacillus fusiformis KNUC404. Crystal aggregates were apparent in the bacterial colonies grown on an agar medium. Stereomicroscopy, scanning electron microscopy, and x-ray diffraction analyses illustrated both the crystal growth and the crystalline structure of the CaCO3 crystals. We used the isolates to improve the compressive strength of concrete mortar cubes and found that KNUC403 offered the best improvement in compressive strength.

  1. Phenylxylylethane (PXE): a high-density, high-flashpoint organic liquid scintillator for applications in low-energy particle and astrophysics experiments

    CERN Document Server

    Rappaport, S; Balata, M; Beau, T; Bellini, G; Benziger, J; Bonetti, S; Brigatti, A; Buck, C; Caccianiga, B; Cadonati, L; Calaprice, F; Cecchet, G; Chen, M; D'Angelo, D; Dadoun, O; De Bari, A; De Bellefon, A; De Kerret, H; Derbin, A; Deutsch, M; Di Credico, A; Elisei, F; Etenko, A; Fernholz, R; Ford, R; Franco, D; Freudiger, B; Galbiati, C; Gärtner, N; Gatti, F; Gazzana, S; Giammarchi, M G; Giugni, D; Goeger-Neff, M; Goretti, A; Grieb, C; HSchuhbeck, K; Haas; Hagner, C; Hampel, W; Harding, E; Hartmann, F X; Hertrich, T; Hess, H; Heusser, G; Ianni, A; Ianni, A M; Kiko, J; Kirsten, T; Korga, G; Korschinek, G; Kozlov, Y; Kryn, D; Laubenstein, M; Lendvai, C; Lombardi, P; Löser, F; Malvezzi, S; Maneira, J; Manno, I; Manuzio, D; Manuzio, G; Martemianov, A; Masetti, F; Mazzucato, U; McCarty, K; Meroni, E; Miramonti, L; Monzani, M E; Musico, P; Niedermeier, L; Oberauer, L; Obolensky, M; Ortica, F; Pallavicini, M; Papp, L; Parmeggiano, S; Perasso, L; Pocar, A; Raghavan, R S; Ranucci, G; Rau, W; Razeto, A; Resconi, E; Sabelnikov, A; Salvo, C; Scardaoni, R; Schimizzi, D; Schönert, S; Seitz, E; Shutt, T; Simgen, H; Skorokhvatov, M; Smirnov, O; Sonnenschein, A; Sotnikov, A; Sukhotin, S; Tarasenkov, V; Tartaglia, R; Testera, G; Vignaud, D; Vogelaar, R B; Von Feilitzsch, F; Vyrodov, V N; Wójcik, M; Zaimidoroga, O A; Zuzel, G

    2004-01-01

    We report on the study of a new liquid scintillator target for neutrino interactions in the framework of the research and development program of the BOREXINO solar neutrino experiment. The scintillator consists of 1,2-dimethyl-4-(1-phenylethyl)-benzene (phenyl-o-xylylethane, PXE) as solvent and 1,4-diphenylbenzene (para-Terphenyl, p-Tp) as primary and 1,4-bis(2-methylstyryl)benzene (bis-MSB) as secondary solute. The density close to that of water and the high flash point makes it an attractive option for large scintillation detectors in general. The study focused on optical properties, radioactive trace impurities and novel purification techniques of the scintillator. Attenuation lengths of the scintillator mixture of 12 m at 430 nm were achieved after purification with an alumina column. A radio carbon isotopic ratio of C-14/C-12 = 9.1 * 10^{-18}$ has been measured in the scintillator. Initial trace impurities, e.g. U-238 at 3.2 * 10^{-14} g/g could be purified to levels below 10^{-17} g/g by silica gel soli...

  2. Theory and simulations of rotating convection

    CERN Document Server

    Barker, Adrian J; Lithwick, Yoram

    2014-01-01

    We study thermal convection in a rotating fluid, with the ultimate goal of explaining the structure of convection zones in rotating stars and planets. We first derive mixing-length theory for rapidly-rotating convection, arriving at the results of Stevenson (1979) via simple physical arguments. The theory predicts the properties of convection as a function of the imposed heat flux and rotation rate, independent of microscopic diffusivities. In particular, it predicts the mean temperature gradient; the rms velocity and temperature fluctuations; and the size of the eddies that dominate heat transport. We test all of these predictions with high resolution three-dimensional hydrodynamical simulations. The results agree remarkably well with the theory across more than two orders of magnitude in rotation rate. For example, the temperature gradient is predicted to scale as the rotation rate to the 4/5th power at fixed flux, and the simulations yield $0.75\\pm 0.06$. We conclude that the mixing length theory is a soli...

  3. New NSO Solar Surface Activity Maps

    Science.gov (United States)

    Henney, C. J.; Harvey, J. W.

    2001-05-01

    Using NSO-Kitt Peak Vacuum Telescope (KPVT) synoptic data, we present several new solar surface activity maps. The motivation is to test conventional wisdom about conditions that are likely to produce solar activity such as flares, coronal mass ejections and high speed solar wind streams. The ultimate goal is to improve real-time, observation-based models for the purpose of predicting solar activity. A large number of maps will eventually be produced based on the wide range of ideas and models of the conditions thought to lead to solar activity events. When data from the new SOLIS instruments becomes available, the range of possible models that can be tested will be greatly expanded. At present, the daily maps include ones that show magnetic field complexity, emerging flux and high speed solar wind sources. As a proxy for local magnetic potential energy, each element of the magnetic complexity map is the distance-weighted rms of the opposing ambient magnetic field. The flux emergence map is the difference between the two most recent absolute magnetic flux images. The solar wind source map is produced from coronal hole area data. The new maps are available on the NSO-Kitt Peak World Wide Web page. This research was supported in part by the Office of Navel Research Grant N00014-91-J-1040. The NSO-Kitt Peak data used here are produced cooperatively by NSF/AURA, NASA/GSFC, and NOAA/SEC.

  4. Pantani di Marco Martinelli: una narrazione polifonica

    Directory of Open Access Journals (Sweden)

    Franco Nasi

    2014-05-01

    Full Text Available In questo articolo si analizza il testo drammaturgico Pantani di Marco Martinelli, una veglia epica teatrale di uno dei più noti eroi dello sport italiano degli ultimi anni. Il dramma rilegge anche la storia di Pantani e ricostruisce gli strani avvenimenti che portarono alla sospensione, alla estromissione del ciclista dal Giro d’Italia nel 1999, e in seguito alla sua profonda crisi psicologica e tragica morte in solitudine in un hotel, a soli 34 anni. Oltre alle modalità proprie della veglia rituale, il dramma utilizza anche i metodi del processo giudiziario, e offre testimonianze dettagliate che conducono a una riflessione critica sulla società italiana negli anni del potere politico di Berlusconi e della sua filosofia del consumismo. I tre diversi generi della retorica classica (epidittico, giudiziario e deliberativo si mescolano in questa complessa tessitura teatrale. Coniugando informazioni (processo e rito (veglia, Martinelli riesce a fondere la tragedia greca (forma mimetica e corale e il moderno teatro di narrazione (forma diegetica monologante. Combinando assieme scene intensamente drammatiche, ricordi di imprese sportive epiche, passaggi giocosi e parodici, e giustapponendo vari stili e registri (giornalistico, lirico, parodico, elegiaco, Martinelli crea momenti di forte “straniamento” e sorpresa, e rende particolarmente efficace il rito funebre, la ricostruzione storica della tragica vicenda di Pantani e la critica all’ideologia che ha dominato l’Italia negli anni di Berlusconi. 

  5. Breaking the 1000-gene barrier for Mimivirus using ultra-deep genome and transcriptome sequencing

    Directory of Open Access Journals (Sweden)

    Claverie Jean-Michel

    2011-03-01

    Full Text Available Abstract Background Mimivirus, a giant dsDNA virus infecting Acanthamoeba, is the prototype of the mimiviridae family, the latest addition to the family of the nucleocytoplasmic large DNA viruses (NCLDVs. Its 1.2 Mb-genome was initially predicted to encode 917 genes. A subsequent RNA-Seq analysis precisely mapped many transcript boundaries and identified 75 new genes. Findings We now report a much deeper analysis using the SOLiD™ technology combining RNA-Seq of the Mimivirus transcriptome during the infectious cycle (202.4 Million reads, and a complete genome re-sequencing (45.3 Million reads. This study corrected the genome sequence and identified several single nucleotide polymorphisms. Our results also provided clear evidence of previously overlooked transcription units, including an important RNA polymerase subunit distantly related to Euryarchea homologues. The total Mimivirus gene count is now 1018, 11% greater than the original annotation. Conclusions This study highlights the huge progress brought about by ultra-deep sequencing for the comprehensive annotation of virus genomes, opening the door to a complete one-nucleotide resolution level description of their transcriptional activity, and to the realistic modeling of the viral genome expression at the ultimate molecular level. This work also illustrates the need to go beyond bioinformatics-only approaches for the annotation of short protein and non-coding genes in viral genomes.

  6. Deep sequencing of virus-infected cells reveals HIV-encoded small RNAs.

    Science.gov (United States)

    Schopman, Nick C T; Willemsen, Marcel; Liu, Ying Poi; Bradley, Ted; van Kampen, Antoine; Baas, Frank; Berkhout, Ben; Haasnoot, Joost

    2012-01-01

    Small virus-derived interfering RNAs (viRNAs) play an important role in antiviral defence in plants, insects and nematodes by triggering the RNA interference (RNAi) pathway. The role of RNAi as an antiviral defence mechanism in mammalian cells has been obscure due to the lack of viRNA detection. Although viRNAs from different mammalian viruses have recently been identified, their functions and possible impact on viral replication remain unknown. To identify viRNAs derived from HIV-1, we used the extremely sensitive SOLiD(TM) 3 Plus System to analyse viRNA accumulation in HIV-1-infected T lymphocytes. We detected numerous small RNAs that correspond to the HIV-1 RNA genome. The majority of these sequences have a positive polarity (98.1%) and could be derived from miRNAs encoded by structured segments of the HIV-1 RNA genome (vmiRNAs). A small portion of the viRNAs is of negative polarity and most of them are encoded within the 3'-UTR, which may represent viral siRNAs (vsiRNAs). The identified vsiRNAs can potently repress HIV-1 production, whereas suppression of the vsiRNAs by antagomirs stimulate virus production. These results suggest that HIV-1 triggers the production of vsiRNAs and vmiRNAs to modulate cellular and/or viral gene expression. PMID:21911362

  7. A study of catasterisms in the 'phaenomena' of Aratus

    Science.gov (United States)

    Rousseau, A.; Dimitrakoudis, S.

    We provide a fresh analysis of the constellations in Aratos Phenomena by using the astronomical program Cybersky (by Stephen Schimpf) to check each reference of constellations within the poem for validity in 2800 BCE and 300 BCE (the later accounting for the broader period of time covering Eudoxus of Cnidus and Aratus of Soli). In each case, the latitude of observation was chose to be 36 North in agreement with the area of the sky that is not covered in the descriptions of Aratus (and contains the unseen constellations for a particular latitude). Each constellation was traced back to its Greek mythological origin through tha various writers of antiquity. Our results are collected in a table of the constellations mentioned by Aratus in his epic poem, with respect to the ancient authors who have mentioned each constellation shaping its myth, the locations on the earth each constellation is associated with and the most likely date of observation according to Aratus description and taking into account precession and the proper motion of stars.

  8. SRAdb: query and use public next-generation sequencing data from within R

    Directory of Open Access Journals (Sweden)

    Zhu Yuelin

    2013-01-01

    Full Text Available Abstract Background The Sequence Read Archive (SRA is the largest public repository of sequencing data from the next generation of sequencing platforms including Illumina (Genome Analyzer, HiSeq, MiSeq, .etc, Roche 454 GS System, Applied Biosystems SOLiD System, Helicos Heliscope, PacBio RS, and others. Results SRAdb is an attempt to make queries of the metadata associated with SRA submission, study, sample, experiment and run more robust and precise, and make access to sequencing data in the SRA easier. We have parsed all the SRA metadata into a SQLite database that is routinely updated and can be easily distributed. The SRAdb R/Bioconductor package then utilizes this SQLite database for querying and accessing metadata. Full text search functionality makes querying metadata very flexible and powerful. Fastq files associated with query results can be downloaded easily for local analysis. The package also includes an interface from R to a popular genome browser, the Integrated Genomics Viewer. Conclusions SRAdb Bioconductor package provides a convenient and integrated framework to query and access SRA metadata quickly and powerfully from within R.

  9. Next-generation sequencing technologies and the application in microbiology-A review%高通量测序技术及其在微生物学研究中的应用

    Institute of Scientific and Technical Information of China (English)

    秦楠; 栗东芳; 杨瑞馥

    2011-01-01

    20世纪70年代发明的核酸测序技术为基因组学及其相关学科的发展做出了巨大贡献,本世纪初发展的以Illumina公司的HiSeq 2000,ABI公司的SOLID,和Roche公司的454技术为代表的高通量测序技术又为基因组学的发展注入了新活力.本文在阐述这些技术的基础上,着重讨论了新一代测序技术在微生物领域中的应用.%Since its invention in 1970s, nucleic acid sequencing technology has contributed tremendously to the genomics advances.The next-generation sequencing technologies, represented by HiSeq 2000 from Illumina, SOLiD from Applied Biosystems and 454 from Roche, re-energized the application of genomics.In this review, we first introduced the next-generation sequencing technologies, then, described their potential applications in the field of microbiology.

  10. Performance comparison of digital microRNA profiling technologies applied on human breast cancer cell lines.

    Directory of Open Access Journals (Sweden)

    Erik Knutsen

    Full Text Available MicroRNA profiling represents an important first-step in deducting individual RNA-based regulatory function in a cell, tissue, or at a specific developmental stage. Currently there are several different platforms to choose from in order to make the initial miRNA profiles. In this study we investigate recently developed digital microRNA high-throughput technologies. Four different platforms were compared including next generation SOLiD ligation sequencing and Illumina HiSeq sequencing, hybridization-based NanoString nCounter, and miRCURY locked nucleic acid RT-qPCR. For all four technologies, full microRNA profiles were generated from human cell lines that represent noninvasive and invasive tumorigenic breast cancer. This study reports the correlation between platforms, as well as a more extensive analysis of the accuracy and sensitivity of data generated when using different platforms and important consideration when verifying results by the use of additional technologies. We found all the platforms to be highly capable for microRNA analysis. Furthermore, the two NGS platforms and RT-qPCR all have equally high sensitivity, and the fold change accuracy is independent of individual miRNA concentration for NGS and RT-qPCR. Based on these findings we propose new guidelines and considerations when performing microRNA profiling.

  11. microRNAs control of in vivo toxicity from graphene oxide in Caenorhabditis elegans.

    Science.gov (United States)

    Wu, Qiuli; Zhao, Yunli; Zhao, Gui; Wang, Dayong

    2014-10-01

    The molecular basis for in vivo graphene oxide (GO) toxicity is still largely unclear. We here used Caenorhabditis elegans to investigate the microRNAs (miRNAs) control of GO toxicity. With the aid of SOLiD sequencing, we identified 23 up-regulated and 8 down-regulated miRNAs in GO-exposed nematodes. Gene ontology and KEGG pathway database analysis implied that these identified miRNAs might be involved in control of many biological processes, and some of them suggest the possible new functions of GO. Functions of the identified miRNAs in regulating the GO toxicity on lifespan were confirmed in the available miRNAs mutants. Moreover, we provide the evidence to raise a hypothesis that GO may reduce lifespan through influencing the functions of insulin/IGF signaling, TOR signaling, and germline signaling pathways controlled by miRNAs. Our results will be helpful for understanding the molecular basis for GO toxicity, and finding clues for useful surface modifications to reduce GO toxicity. From the clinical editor: In this study, toxicity of graphene oxide is studied in a Caenorhabditis elegans model via microRNA analysis. The authors report that multiple important pathways are influenced by GO and raise a hypothesis that GO may reduce lifespan through influencing the functions of insulin/IGF signaling, TOR signaling, and germline signaling pathways.

  12. The dazed and confused identity of Agassiz's land tortoise, Gopherus agassizii (Testudines, Testudinidae) with the description of a new species, and its consequences for conservation

    Science.gov (United States)

    Murphy, Robert K.; Berry, Kristin; Edwards, Taylor; Leviton, Alan E.; Lathrop, Amy; Riedle, J. Daren

    2011-01-01

    We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii Cooper. The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of G. lepidocephalus Ottley et Velázques Solis, 1989 from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because G. lepidocephalus is from the Mojavian population no names are available for the Sonoran species. Thus, a new species, Gopherus morafkai sp. n., is named and this action reduces the distribution of G. agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of G. agassizii, which may deserve a higher level of protection.

  13. Two new species of philometrid nematodes (Nematoda: Philometridae) in Epinephelus coioides (Hamilton, 1822) from the South Bali Sea, Indonesia.

    Science.gov (United States)

    Dewi, Kartika; Palm, Harry W

    2013-01-25

    Based on light and scanning electron microscopy, two new species of philometrid nematodes, Spirophilometra endangae sp. nov. and Philometra epinepheli sp. nov. (Nematoda: Dracunculoidea: Philometridae) are described from Epinephelus coioides (Hamilton, 1822) (Perciformes: Serranidae) from the South Bali Sea, Indonesia. Spirophilometra endangae sp. nov. was isolated from the fins of E. coioides. The new species can be distinguished from the most closely related S. eichleri Parukhin, 1971 by a larger total body length and the site of infection in the host. The new species differs from S. centropomi (Caballero, 1974) also in the larger body size of the gravid females and the site of infection in the host. S. en-dangae sp. nov. differs from S. pacifica (Moravec, Santana-Pineros, Gonzales-Solis & Torres-Huerta, 2007) in the struc-ture and arrangement of the spines on the middle part of the body, the infection site of the worm, the type host and the zoogeographical host distribution. Philometra epinepheli sp. nov. differs from all other Philometra spp. congeners so far recorded from Ephinepelus groupers in the total body length and the site of infection. This is the first opercula-infecting species of Philometra described from the fish family Serranidae.

  14. Solution of the KdV Equation Based on COMSOL Multiphysics%基于COMSOL Multiphysics求解KdV方程

    Institute of Scientific and Technical Information of China (English)

    糜凯华

    2014-01-01

    In order to obtain numerical solutions of the KdV equation,COMSOL Multiphysics software based on finite element method established a finite element model and solved the distribution of solitary waves at various points in time. The results can study the propagation of solitary waves. Simulation results indicate that the soli-tary wave does not occur in isolation. Solitary waves can be found within a certain space where collisions occur as well as reproduced by representing as a three dimensional map distribution. Two different speed and ampli-tude solitons collided and did not affect each other.%为了得到KdV方程的数值解,基于有限单元法的COMSOL Multiphysics软件建立有限元模型并求解孤立波在各个时间点的分布情况,以研究孤立波的传播特性。数值模拟结果表明:孤立波不会发生消散,以三维图的分布形式表示,可以发现孤立子在一定的空间内会发生碰撞以及发生重现,也即两个不同速度和振幅的孤子相互碰撞、相互穿过,互相之间没有影响。

  15. Enhanced crude oil biodegradation in soil via biostimulation.

    Science.gov (United States)

    Al-Saleh, Esmaeil; Hassan, Ali

    2016-08-01

    Research on feasible methods for the enhancement of bioremediation in soil contaminated by crude oil is vital in oil-exporting countries such as Kuwait, where crude oil is a major pollutant and the environment is hostile to biodegradation. This study investigated the possibility of enhancing crude oil bioremediation by supplementing soil with cost-effective organic materials derived from two widespread locally grown trees, Conocarpus and Tamarix. Amendments in soils increased the counts of soil microbiota by up to 98% and enhanced their activity by up to 95.5%. The increase in the biodegradation of crude oil (75%) and high levels of alkB expression substantiated the efficiency of the proposed amendment technology for the bioremediation of hydrocarbon-contaminated sites. The identification of crude-oil-degrading bacteria revealed the dominance of the genus Microbacterium (39.6%), Sphingopyxis soli (19.3%), and Bordetella petrii (19.6%) in unamended, Conocarpus-amended, and Tamarix-amended contaminated soils, respectively. Although soil amendments favored the growth of Gram-negative bacteria and reduced bacterial diversity, the structures of bacterial communities were not significantly altered. PMID:26854134

  16. An Empirically Driven Time-Dependent Model of the Solar Wind

    Science.gov (United States)

    Linker, Jon A.; Caplan, Ronald M.; Downs, Cooper; Lionello, Roberto; Riley, Pete; Mikic, Zoran; Henney, Carl J.; Arge, Charles N.; Kim, Tae; Pogorelov, Nikolai

    2016-05-01

    We describe the development and application of a time-dependent model of the solar wind. The model is empirically driven, starting from magnetic maps created with the Air Force Data Assimilative Photospheric flux Transport (ADAPT) model at a daily cadence. Potential field solutions are used to model the coronal magnetic field, and an empirical specification is used to develop boundary conditions for an MHD model of the solar wind. The time-dependent MHD simulation shows classic features of stream structure in the interplanetary medium that are seen in steady-state models; it also shows time evolutionary features that do not appear in a steady-state approach. The model results compare reasonably well with 1 AU OMNI observations. Data gaps when SOLIS magnetograms were unavailable hinder the model performance. The reasonable comparisons with observations suggest that this modeling approach is suitable for driving long term models of the outer heliosphere. Improvements to the ingestion of magnetograms in flux transport models will be necessary to apply this approach in a time-dependent space weather model.

  17. Single-neuron transcriptome and methylome sequencing for epigenomic analysis of aging.

    Science.gov (United States)

    Moroz, Leonid L; Kohn, Andrea B

    2013-01-01

    Enormous heterogeneity in transcription and signaling is the feature that slows down progress in our understanding of the mechanisms of normal aging and age-related diseases. This is critical for neurobiology of aging where the enormous diversity of neuronal populations presents a significant challenge in experimental design. Here, we introduce Aplysia as a model for genomic analysis of aging at the single-cell level and provide protocols for integrated transcriptome and methylome profiling of individually identified neurons during the aging process. These single-cell RNA-seq and DNA methylation assays (methyl-capture/methyl enrichment) are compatible with all major next generation sequencing platforms (we used Roche/454 and SOLiD/Life Technologies as illustrative examples) and can be used to integrate an epigenetic signature with transcriptional output. The described sequencing library construction protocol provides both quantitative and directional information from transcriptional profiling of individual cells. Our results also confirm that different copies of DNA in polyploid Aplysia neurons behave similarly with respect to their DNA methylation.

  18. Undeserving mothers? Shifting rationalities in the maternal healthcare of undocumented Nicaraguan migrants in Costa Rica.

    Science.gov (United States)

    Dos Santos, Sara Leon Spesny

    2015-01-01

    The case of Nicaraguan migrants in Costa Rica is emblematic of the issues that immigration generates in host countries. Undocumented Nicaraguan women seeking maternal care constitute a key challenge to the universal coverage of Costa Rica's health system. Can the long-standing commitment to universality, solidarity and equality expressed in the legislation be translated into practice? Discourses of health professionals in Costa Rica reveal a contradiction between merit and prejudice in prenatal and delivery care. Here, I present qualitative research based on semi-structured interviews with physicians and nurses at a Costa Rican National Hospital. The data show that migrant women, rejected from primary care, do find help in emergency services, but not without difficulties, as they must engage in individual negotiations centred on their bodies. The discourses of health providers reflect an ambivalence between the perceived undeservingness of undocumented migrant women and the medical realisation that two lives are at risk. While the foetus often evokes compassion, the mother commonly provokes repression, as specific and shifting rationalities reflect new moral regimes that are applied to this population. Women are perceived as being 'illegal', 'immoral' and 'irrational', and the baby, although legally Costa Rican due to jus solis policy, embodies 'the other'. Ultimately, otherness frames perceptions of deservingness of maternal care for undocumented migrant women in Costa Rica. PMID:25639299

  19. Příprava a hodnocení konverzních fluoridových povlaků na tvářené hořčíkové slitině AZ31

    OpenAIRE

    Matějka, Lukáš

    2016-01-01

    Cílem práce je optimalizace parametrů přípravy fluoridového konverzního povlaku na tvářené hořčíkové slitině AZ31v tavenině Na[BF4]. Sledovanými faktory pro stanovení kvality připravených povlaků jsou homogenita povrchu povlaku na broušených a leštěných vzorcích hořčíkové slitiny, mechanické vlastnosti povlaků (adheze a tvrdost) a tloušťka povlaku. Hlavním sledovaným parametrem byla změna těchto vlastností při expozici vzorků tavenině soli na různou dobu. Povrchy povlaků byly dokumentovány na...

  20. Study of Flow Field and Separation Efficiency of a High-efficiency Dynamic Cyclone Separator%一种高效动态旋风分离器流场与分离效率研究

    Institute of Scientific and Technical Information of China (English)

    樊宁; 刘根凡; 毛锐; 翁建松

    2015-01-01

    为了研究对超细颗粒捕集能力很强的新型高效动态旋风分离器———气固离心分离与排气一体机的流场特点与分离性能,利用 Fluent 软件对一体机内部气相流场与颗粒相进行数值模拟,并计算出各工况下的分级效率。结果显示,该机对可入肺超细颗粒捕集能力远强于传统旋风分离器,并且对工况波动的适应能力较强。%Gas-solid centrifugal separation and exhausting equipment (CCSEE)is a kind of dy-namic cyclone separator with a remarkable capture effect for ultrafine particles.In order to ex-plore the flow field characteristics and separation efficiency of the new kind of separator,the soft-ware Fluent is used to simulate the flow filed and the solid phase inner CCSEE and the classifica-tion efficiency is calculated under different working conditions.The numerical simulation shows that CCSEE has a stronger ability on capturing ultrafine particles PM2.5 than that conventional cyclone separator has,and CCSEE has a better adaption to the working condition fluctuations.

  1. Characterization of DNA variants in the human kinome in breast cancer

    Science.gov (United States)

    Agarwal, Divyansh; Qi, Yuan; Jiang, Tingting; Liu, Xiuping; Shi, Weiwei; Wali, Vikram B.; Turk, Benjamin; Ross, Jeffrey S.; Fraser Symmans, W.; Pusztai, Lajos; Hatzis, Christos

    2015-09-01

    Kinases play a key role in cancer biology, and serve as potential clinically useful targets for designing cancer therapies. We examined nucleic acid variations in the human kinome and several known cancer-related genes in breast cancer. DNA was extracted from fine needle biopsies of 73 primary breast cancers and 19 metastatic lesions. Targeted sequencing of 518 kinases and 68 additional cancer related genes was performed using the SOLiD sequencing platform. We detected 1561 unique, non-synonymous variants in kinase genes in the 92 cases, and 74 unique variants in 43 kinases that were predicted to have major functional impact on the protein. Three kinase groups—CMGC, STE and TKL—showed greater mutational load in metastatic compared to primary cancer samples, however, after correction for multiple testing the difference was significant only for the TKL group (P = 0.04). We also observed that a higher proportion of histologic grade 1 and 2 cases had high functional impact variants in the SCYL2 gene compared with grade 3 cases. Our findings indicate that individual breast cancers harbor a substantial number of potentially functionally important nucleotide variations in kinase genes, most of which are present in unique combinations and include both somatic and germline functional variants.

  2. Il rapporto tra laicità e neutralità: una questione concettuale?

    Directory of Open Access Journals (Sweden)

    Corrado Del Bò

    2014-10-01

    Full Text Available Per la cortesia dell’Autore e dell’Editore il testo riproduce - tranne il paragrafo introduttivo, e con i soli aggiustamenti formali necessari a renderlo saggio autonomo - il capitolo 5 del volume monografico La neutralità necessaria. Liberalismo e religione nell’età del pluralismo(ETS Edizioni, Pisa, 2014.SOMMARIO: 1. Introduzione - 2. Su alcuni equivoci nel dibattito italiano sulla laicità - 3. Laicità e neutralità: un rapporto complicato - 4. La laicità della Corte costituzionale - 5. Conclusioni.ABSTRACT: This article, which is the chapter five of the book La neutralità necessaria. Liberalismo e religione nell’età del pluralismo (ETS, 2014, tries to identify what is the link between the idea of neutrality and secularism, suggesting that this link depends on the various possible interpretations of neutrality. Moreover, it is argued that we should avoid to reduce the issue to a nominal disputation about the meaning of the words and move our attention to the more fundamental problem about the relationship between politics and religion in a secular State.

  3. Fermi-Pasta-Ulam recurrence and modulation instability

    CERN Document Server

    Kuznetsov, E A

    2016-01-01

    We give a qualitative explanation of the analog of the Fermi-Pasta-Ulam (FPU) recurrence in a one-dimensional focusing nonlinear Schrodinger equation (NLSE). That recurrence can be considered as a result of the nonlinear development of modulation instability. All known exact localized solitons-type solutions describing propagation on the background of the modulationally unstable condensate show the recurrence to the condensate state after its interaction with solitons. The condensate state locally recovers its original form with the same amplitude but a different phase after soliton leave its initial region. This is the analog of the FPU recurrence for the NLSE. Based on the integrability of the NLSE, we demonstrate that the FPU recurrence takes place not only for condensate but for more general solution in the form of the cnoidal wave. This solution is periodic in space and can be represented as a solitonic lattice. That lattice reduces to isolated soliton solution in the limit of large distance between soli...

  4. Borders and Legal Criteria for Acquiring Nationality

    Directory of Open Access Journals (Sweden)

    María Elósegui Itxaso

    2008-09-01

    Full Text Available Legal criteria for acquiring nationality are crucial in the integration of persons into society, since they provide access to the right to vote. Until now, the criteria most frequently used are those of ius soli (birth and ius sanguinis (nationality is inherited from the parents, which comply with previous anthropological approaches and which jurists accept without reflection, or consider to be unshakeable traditions.The author’s proposal in this article is to accept that some of these legal criteria should be reformed, though not in an anarchic manner. On one hand, some of the ethnic criteria may be respected, but on the other, the processes of acquiring nationality should be streamlined by accepting the desire of persons wanting to change their nationality on moving to a new country of residence. Meanwhile, we must establish channels of demand for accepting the democratic values and legal system of the welcoming country, as a result of which it would be fair to call for a prior learning period before the rights to nationality and suffrage are granted. The author also adds – and accepts as being a fundamental element – some of Habermas’ inclusion theses, though she stresses that this discourse should be organised into two specific, feasible legal solutions or rather, in a realistic manner.

  5. Fixed Ammonium COntents and NH4+ Fixation Capacities of Some Cultivated Soils in China

    Institute of Scientific and Technical Information of China (English)

    WENQI-XIAO; CHENGLI-LI; 等

    1995-01-01

    Fixed ammonium contents and NH4+ fixation capacities of some representative cultivated solis collected from 16 provinces of China were studied.Results showed that the contents of fixed ammonium in soils ranged from 35 to 573 mg N kg-1,with an average of 198mg Nkg-1.The content of fixed ammonium correlated very significantly with mica content for tropical and subtropical soils,whereas this was not the case for soils in the temperate zone.At the end of K-exhausting experiment the fixed ammonium content increased in most soils studied.However,it decreased in smom temperate soils.Generally,fixation of added NH4+ could not be found either before or after K-exhausting experiment for highly weathered soils,including tropical soils and soils derived from granite-gneiss or Quaternary red clays in the subtropic zone,while for most soils in the Yangtze River dalta the NH4+ fixation capacity was rather high and increased significantly in the K-exhausted soils.

  6. Metagenome of a microbial community inhabiting a metal-rich tropical stream sediment.

    Directory of Open Access Journals (Sweden)

    Patrícia S Costa

    Full Text Available Here, we describe the metagenome and functional composition of a microbial community in a historically metal-contaminated tropical freshwater stream sediment. The sediment was collected from the Mina Stream located in the Iron Quadrangle (Brazil, one of the world's largest mining regions. Environmental DNA was extracted and was sequenced using SOLiD technology, and a total of 7.9 Gbp was produced. A taxonomic profile that was obtained by comparison to the Greengenes database revealed a complex microbial community with a dominance of Proteobacteria and Parvarcheota. Contigs were recruited by bacterial and archaeal genomes, especially Candidatus Nitrospira defluvii and Nitrosopumilus maritimus, and their presence implicated them in the process of N cycling in the Mina Stream sediment (MSS. Functional reconstruction revealed a large, diverse set of genes for ammonium assimilation and ammonification. These processes have been implicated in the maintenance of the N cycle and the health of the sediment. SEED subsystems functional annotation unveiled a high degree of diversity of metal resistance genes, suggesting that the prokaryotic community is adapted to metal contamination. Furthermore, a high metabolic diversity was detected in the MSS, suggesting that the historical arsenic contamination is no longer affecting the prokaryotic community. These results expand the current knowledge of the microbial taxonomic and functional composition of tropical metal-contaminated freshwater sediments.

  7. Sunspot positions, areas, and group tilt angles for 1611-1631 from observations by Christoph Scheiner

    CERN Document Server

    Arlt, R; Schmiel, C; Spada, F

    2016-01-01

    Digital images of the observations printed in the books "Rosa Ursina sive solis" and "Prodromus pro sole mobili" by Christoph Scheiner as well as the drawings from Scheiner's letters to Marcus Welser are analysed in order to obtain information on positions and sizes of sunspots that appeared before the Maunder minimum. In most cases, the given orientation of the ecliptic is used to set up the heliographic coordinate system for the drawings. Positions and sizes are measured manually on the screen. Very early drawings have no indication of their orientation. A rotational matching using common spots of adjacent days is used in some cases, while in other cases, the assumption of images being aligned with a zenith-horizon coordinate system appeared to be the most probable. In total, 8167 sunspots were measured. A distribution of sunspot latitudes versus time (butterfly diagram) is obtained for Scheiner's observations. The observations of 1611 are very inaccurate, the drawings of 1612 have at least an indication of...

  8. Extensive sequencing of seven human genomes to characterize benchmark reference materials.

    Science.gov (United States)

    Zook, Justin M; Catoe, David; McDaniel, Jennifer; Vang, Lindsay; Spies, Noah; Sidow, Arend; Weng, Ziming; Liu, Yuling; Mason, Christopher E; Alexander, Noah; Henaff, Elizabeth; McIntyre, Alexa B R; Chandramohan, Dhruva; Chen, Feng; Jaeger, Erich; Moshrefi, Ali; Pham, Khoa; Stedman, William; Liang, Tiffany; Saghbini, Michael; Dzakula, Zeljko; Hastie, Alex; Cao, Han; Deikus, Gintaras; Schadt, Eric; Sebra, Robert; Bashir, Ali; Truty, Rebecca M; Chang, Christopher C; Gulbahce, Natali; Zhao, Keyan; Ghosh, Srinka; Hyland, Fiona; Fu, Yutao; Chaisson, Mark; Xiao, Chunlin; Trow, Jonathan; Sherry, Stephen T; Zaranek, Alexander W; Ball, Madeleine; Bobe, Jason; Estep, Preston; Church, George M; Marks, Patrick; Kyriazopoulou-Panagiotopoulou, Sofia; Zheng, Grace X Y; Schnall-Levin, Michael; Ordonez, Heather S; Mudivarti, Patrice A; Giorda, Kristina; Sheng, Ying; Rypdal, Karoline Bjarnesdatter; Salit, Marc

    2016-06-07

    The Genome in a Bottle Consortium, hosted by the National Institute of Standards and Technology (NIST) is creating reference materials and data for human genome sequencing, as well as methods for genome comparison and benchmarking. Here, we describe a large, diverse set of sequencing data for seven human genomes; five are current or candidate NIST Reference Materials. The pilot genome, NA12878, has been released as NIST RM 8398. We also describe data from two Personal Genome Project trios, one of Ashkenazim Jewish ancestry and one of Chinese ancestry. The data come from 12 technologies: BioNano Genomics, Complete Genomics paired-end and LFR, Ion Proton exome, Oxford Nanopore, Pacific Biosciences, SOLiD, 10X Genomics GemCode WGS, and Illumina exome and WGS paired-end, mate-pair, and synthetic long reads. Cell lines, DNA, and data from these individuals are publicly available. Therefore, we expect these data to be useful for revealing novel information about the human genome and improving sequencing technologies, SNP, indel, and structural variant calling, and de novo assembly.

  9. Correlation Between Sunspot Number and ca II K Emission Index

    CERN Document Server

    Bertello, Luca; Tlatov, Andrey; Singh, Jagdev

    2016-01-01

    Long-term synoptic observations in the resonance line of Ca II K constitute a fundamental database for a variety of retrospective analyses of the state of the solar magnetism. Synoptic Ca II K observations began in late 1904 at the Kodaikanal Observatory, in India. In early 1970s, the National Solar Observatory (NSO) at Sacramento Peak (USA) started a new program of daily Sun-as-a-star observations in the Ca II K line. Today the NSO is continuing these observations through its Synoptic Optical Long-term Investigations of the Sun (SOLIS) facility. These different data sets can be combined into a single disk-integrated Ca II K index time series that describes the average properties of the chromospheric emission over several solar cycles. We present such a Ca II K composite and discuss its correlation with the new entirely revised sunspot number data series. For this preliminary investigation, the scaling factor between pairs of time series was determined assuming a simple linear model for the relationship betwe...

  10. Nonlinear force-free and potential field models of active-region and global coronal fields during the Whole Heliospheric Interval

    CERN Document Server

    Petrie, Gordon; Amari, Tahar

    2010-01-01

    Between 2008/3/24 and 2008/4/2, the three active regions NOAA active regions 10987, 10988 and 10989 were observed daily by the Synoptic Optical Long-term Investigations of the Sun (SOLIS) Vector Spectro-Magnetograph (VSM) while they traversed the solar disk. We use these measurements and the nonlinear force-free magnetic field code XTRAPOL to reconstruct the coronal magnetic field for each active region and compare model field lines with images from the Solar Terrestrial RElations Observatory (STEREO) and Hinode X-ray Telescope (XRT) telescopes. Synoptic maps made from continuous, round-the-clock Global Oscillations Network Group (GONG) magnetograms provide information on the global photospheric field and potential-field source-surface models based on these maps describe the global coronal field during the Whole Heliospheric Interval (WHI) and its neighboring rotations. Features of the modeled global field, such as the coronal holes and streamer belt locations, are discussed in comparison with extreme ultra-v...

  11. Un Resultat Gravimetrique pour la Renaissance de la Theorie Corpusculaire "An Experimental Gravimetric Result for the Revival of Corpuscular Theory"

    CERN Document Server

    Duval, Maurice

    2005-01-01

    The gravitational phenomenon, founded on the assumption of absorption of a flux of gravitons through the matter, led to a law of attraction comprising a term of attenuation of gravity. The attenuation effect, which depends on the distribution of mass elements into the Sun, was compared with the relativistic effect for the orbital elements of planets. The calculations carried out with our modified law of Newton, lead to a perfect agreement for the advance of the perihelion of planets, and give an interaction cross section with the matter of 3.2x10^-17 m2/kg (approximately 10^-40 cm2/nucleon). The effect of attenuation during a solar eclipse will produce a local reduction in the soli-lunar attraction of 0.13 microgal. This small variation of gravity during the eclipse can lead to distortions of the geoid of about ten millimetres of which effect on gravity, estimated at a few microgals, is compatible with the observed gravitational anomalies. The gravimetric measurements taken in the area of Montreal during the ...

  12. Maximum power analysis of photovoltaic module in Ramadi city

    Directory of Open Access Journals (Sweden)

    Majid Shahatha Salim, Jassim Mohammed Najim, Salih Mohammed Salih

    2013-01-01

    Full Text Available Performance of photovoltaic (PV module is greatly dependent on the solar irradiance, operating temperature, and shading. Solar irradiance can have a significant impact on power output of PV module and energy yield. In this paper, a maximum PV power which can be obtain in Ramadi city (100km west of Baghdad is practically analyzed. The analysis is based on real irradiance values obtained as the first time by using Soly2 sun tracker device. Proper and adequate information on solar radiation and its components at a given location is very essential in the design of solar energy systems. The solar irradiance data in Ramadi city were analyzed based on the first three months of 2013. The solar irradiance data are measured on earth's surface in the campus area of Anbar University. Actual average data readings were taken from the data logger of sun tracker system, which sets to save the average readings for each two minutes and based on reading in each one second. The data are analyzed from January to the end of March-2013. Maximum daily readings and monthly average readings of solar irradiance have been analyzed to optimize the output of photovoltaic solar modules. The results show that the system sizing of PV can be reduced by 12.5% if a tracking system is used instead of fixed orientation of PV modules.

  13. BIORREMEDIACIÓN DE UN SUELO CON DIESEL MEDIANTE EL USO DE MICROORGANISMOS AUTÓCTONOS

    Directory of Open Access Journals (Sweden)

    ARRIETA RAMÍREZ OLGA MARIA

    2012-07-01

    Full Text Available En este estudio, se aisló y caracterizó bioquímica y molecularmente un consorcio bacteriano capaz de degradar los diferentes hidrocarburos presentes en un combustible diesel,conformado por los siguientes géneros: Enterobacter sp, Bacillus sp, Staphylococcus aureus, Sanguibacter soli, Arthrobacter sp y Flavobacterium sp, a partir de un suelo contaminado con diesel a escala de laboratorio, y tratado mediante 2 tecnologías de biorremediación: atenuación natural y bioestimulación. Se definió como parámetro de control la concentración de Hidrocarburos Totales del Petróleo (HTP y para el cual, se obtuvo una reducción en la concentración en un periodo de 4 meses de 36,86% para atenuación natural y 50,99% para bioestimulación. La medición de la eficiencia de remoción de hidrocarburos se cuantificó por cromatografía de gases acoplada a masas (GC-MS.

  14. CORPORATE IDENTITY IN HANDLING A BUSINESS ENTITY

    Directory of Open Access Journals (Sweden)

    Margarita Mikhaylovna OZEROVA

    2015-01-01

    Full Text Available The paper explored urgency in seeking a personal identity for a corporate body. A Corporate identity gains in im-portance while upgrading the corporate culture. The corpo-rate identity consolidates the members of the staff for the sake of the common mission that makes for both a well-balanced development of an enterprise in economy terms and every member of the team, while facilitating awareness of the proper conduct required, i.e. showing respect, soli-darity and goodwill to the colleagues. The paper also speci-fied the properties, characteristics and basic duties of the corporate identity appearing for a an interface that is capa-ble not only of encouraging a productive activity but also helps employees realize their personal value for the com-mon cause. The study mentioned that even in case of one and only corporate identity, one can assume not only in-crease of labour productivity, but also some positive atti-tude changes in the employees to their professional duties performance, an increased interest in achieving jointly advancement of the company, and attitude changes in the scale of values and orientations.

  15. Parenting under the influence: the effects of opioids, alcohol and cocaine on mother-child interaction.

    Science.gov (United States)

    Slesnick, Natasha; Feng, Xin; Brakenhoff, Brittany; Brigham, Gregory S

    2014-05-01

    Nearly 20% of adults receiving treatment for a substance use disorder live with their minor children (Stanger et al., 1999) and women in drug use treatment are twice as likely as men to have children in their household (Wechsberg et al., 1998). Parental drug use impacts the family through reduced family resources such as money and food, and researchers consistently note parenting deficits among substance users (Solis, Shadur, Burns, & Hussong, 2012). Little is known about differences in parenting and mother-child interaction among mothers with different drugs of choice or among mothers of older children, between 8 and 16 years. This study reports the findings from a sample of treatment seeking opioid, alcohol and cocaine using mothers and their 8-16-year-old child. Findings from a mother-child observational task and self-reported parenting measure indicated less undermining autonomy and higher mother maternal acceptance among opioid compared to alcohol addicted mothers. African American mothers were observed to have fewer negative interactional behaviors than Whites and both African American mothers and children self-reported higher firm control and maternal acceptance. Overall, mothers appeared to struggle with effective discipline with older versus younger children. Findings offer useful information to clinicians seeking to effectively tailor their interventions to women and children who present with different drugs of abuse, race/culture and developmental stage of child. PMID:24589871

  16. The influence of reducing and oxidising agents on rheology of wheat flour dough

    OpenAIRE

    Pečivová, Pavlína; Pavlínek, Vladimír; Hrabě, Jan; Kráčmar, Stanislav

    2008-01-01

    Aminokyseliny jsou stavebními bloky bílkovin a proteinů. Mají široké rozpětí užití v potravinářských procesech a manipulacích s nutrienty, dietními doplňky, obohacovači vůně, náhražkami soli, zabarvovači, antioxidanty, konzervovadly, tvarovači, posilovači a kypřidly na těsto. Je známo, že kvalita tvorby pšeničnéhotěsta záleží jak na kvantitě, tak na kvalitě jeho glutenových bílkovin. Gluten je visko elastický bílkovinný komplex mající jak elastické, tak viskózní vlastnosti. Gluten obsahuje dv...

  17. Testing an astronomically-based decadal-scale empirical harmonic climate model versus the IPCC (2007) general circulation climate models

    CERN Document Server

    Scafetta, Nicola

    2012-01-01

    We compare the performance of a recently proposed empirical climate model based on astronomical harmonics against all available general circulation climate models (GCM) used by the IPCC (2007) to interpret the 20th century global surface temperature. The proposed model assumes that the climate is resonating with, or synchronized to a set of natural harmonics that have been associated to the solar system planetary motion, mostly determined by Jupiter and Saturn. We show that the GCMs fail to reproduce the major decadal and multidecadal oscillations found in the global surface temperature record from 1850 to 2011. On the contrary, the proposed harmonic model is found to well reconstruct the observed climate oscillations from 1850 to 2011, and it is able to forecast the climate oscillations from 1950 to 2011 using the data covering the period 1850-1950, and vice versa. The 9.1-year cycle is shown to be likely related to a decadal Soli/Lunar tidal oscillation, while the 10-10.5, 20-21 and 60-62 year cycles are sy...

  18. High-throughput sequencing of black pepper root transcriptome

    Directory of Open Access Journals (Sweden)

    Gordo Sheila MC

    2012-09-01

    Full Text Available Abstract Background Black pepper (Piper nigrum L. is one of the most popular spices in the world. It is used in cooking and the preservation of food and even has medicinal properties. Losses in production from disease are a major limitation in the culture of this crop. The major diseases are root rot and foot rot, which are results of root infection by Fusarium solani and Phytophtora capsici, respectively. Understanding the molecular interaction between the pathogens and the host’s root region is important for obtaining resistant cultivars by biotechnological breeding. Genetic and molecular data for this species, though, are limited. In this paper, RNA-Seq technology has been employed, for the first time, to describe the root transcriptome of black pepper. Results The root transcriptome of black pepper was sequenced by the NGS SOLiD platform and assembled using the multiple-k method. Blast2Go and orthoMCL methods were used to annotate 10338 unigenes. The 4472 predicted proteins showed about 52% homology with the Arabidopsis proteome. Two root proteomes identified 615 proteins, which seem to define the plant’s root pattern. Simple-sequence repeats were identified that may be useful in studies of genetic diversity and may have applications in biotechnology and ecology. Conclusions This dataset of 10338 unigenes is crucially important for the biotechnological breeding of black pepper and the ecogenomics of the Magnoliids, a major group of basal angiosperms.

  19. Management of High-Throughput DNA Sequencing Projects: Alpheus.

    Science.gov (United States)

    Miller, Neil A; Kingsmore, Stephen F; Farmer, Andrew; Langley, Raymond J; Mudge, Joann; Crow, John A; Gonzalez, Alvaro J; Schilkey, Faye D; Kim, Ryan J; van Velkinburgh, Jennifer; May, Gregory D; Black, C Forrest; Myers, M Kathy; Utsey, John P; Frost, Nicholas S; Sugarbaker, David J; Bueno, Raphael; Gullans, Stephen R; Baxter, Susan M; Day, Steve W; Retzel, Ernest F

    2008-12-26

    High-throughput DNA sequencing has enabled systems biology to begin to address areas in health, agricultural and basic biological research. Concomitant with the opportunities is an absolute necessity to manage significant volumes of high-dimensional and inter-related data and analysis. Alpheus is an analysis pipeline, database and visualization software for use with massively parallel DNA sequencing technologies that feature multi-gigabase throughput characterized by relatively short reads, such as Illumina-Solexa (sequencing-by-synthesis), Roche-454 (pyrosequencing) and Applied Biosystem's SOLiD (sequencing-by-ligation). Alpheus enables alignment to reference sequence(s), detection of variants and enumeration of sequence abundance, including expression levels in transcriptome sequence. Alpheus is able to detect several types of variants, including non-synonymous and synonymous single nucleotide polymorphisms (SNPs), insertions/deletions (indels), premature stop codons, and splice isoforms. Variant detection is aided by the ability to filter variant calls based on consistency, expected allele frequency, sequence quality, coverage, and variant type in order to minimize false positives while maximizing the identification of true positives. Alpheus also enables comparisons of genes with variants between cases and controls or bulk segregant pools. Sequence-based differential expression comparisons can be developed, with data export to SAS JMP Genomics for statistical analysis. PMID:20151039

  20. Digital PCR provides sensitive and absolute calibration for high throughput sequencing

    Directory of Open Access Journals (Sweden)

    Fan H Christina

    2009-03-01

    Full Text Available Abstract Background Next-generation DNA sequencing on the 454, Solexa, and SOLiD platforms requires absolute calibration of the number of molecules to be sequenced. This requirement has two unfavorable consequences. First, large amounts of sample-typically micrograms-are needed for library preparation, thereby limiting the scope of samples which can be sequenced. For many applications, including metagenomics and the sequencing of ancient, forensic, and clinical samples, the quantity of input DNA can be critically limiting. Second, each library requires a titration sequencing run, thereby increasing the cost and lowering the throughput of sequencing. Results We demonstrate the use of digital PCR to accurately quantify 454 and Solexa sequencing libraries, enabling the preparation of sequencing libraries from nanogram quantities of input material while eliminating costly and time-consuming titration runs of the sequencer. We successfully sequenced low-nanogram scale bacterial and mammalian DNA samples on the 454 FLX and Solexa DNA sequencing platforms. This study is the first to definitively demonstrate the successful sequencing of picogram quantities of input DNA on the 454 platform, reducing the sample requirement more than 1000-fold without pre-amplification and the associated bias and reduction in library depth. Conclusion The digital PCR assay allows absolute quantification of sequencing libraries, eliminates uncertainties associated with the construction and application of standard curves to PCR-based quantification, and with a coefficient of variation close to 10%, is sufficiently precise to enable direct sequencing without titration runs.

  1. Comparison of microRNA deep sequencing of matched formalin-fixed paraffin-embedded and fresh frozen cancer tissues.

    Directory of Open Access Journals (Sweden)

    Wei Meng

    Full Text Available MicroRNAs regulate several aspects of tumorigenesis and cancer progression. Most cancer tissues are archived formalin-fixed and paraffin-embedded (FFPE. While microRNAs are a more stable form of RNA thought to withstand FFPE-processing and degradation there is only limited evidence for the latter assumption. We examined whether microRNA profiling can be successfully conducted on FFPE cancer tissues using SOLiD ligation based sequencing. Tissue storage times (2-9 years appeared to not affect the number of detected microRNAs in FFPE samples compared to matched frozen samples (paired t-test p>0.7. Correlations of microRNA expression values were very high across microRNAs in a given sample (Pearson's r = 0.71-0.95. Higher variance of expression values among samples was associated with higher correlation coefficients between FFPE and frozen tissues. One of the FFPE samples in this study was degraded for unknown reasons with a peak read length of 17 nucleotides compared to 21 in all other samples. The number of detected microRNAs in this sample was within the range of microRNAs detected in all other samples. Ligation-based microRNA deep sequencing on FFPE cancer tissues is feasible and RNA degradation to the degree observed in our study appears to not affect the number of microRNAs that can be quantified.

  2. Validation of Coronal and Heliospheric Models for Quasi-Steady Solar Wind: WSA-Enlil, MAS-Enlil, SWMF, and IPS Tomography Models

    Science.gov (United States)

    Jian, L.; MacNeice, P. J.; Taktakishvili, A.; Evans, R. M.; Odstrcil, D.; Arge, C. N.; Jackson, B. V.; Yu, H. S.; Riley, P.; Sokolov, I.

    2014-12-01

    Multiple coronal and heliospheric models have been recently upgraded at the Community Coordinated Modeling Center (CCMC), including the Wang-Sheely-Arge (WSA)-Enlil, MHD-around-a-sphere (MAS)-Enlil, space weather modeling framework (SWMF), and interplanetary scintillation (IPS) tomography models. We run these models for Carrington rotations 2056 - 2062 (April - November 2007), in which the solar wind was in the late declining phase and Ulysses had a fast latitudinal scan at 1.4 - 1.8 AU. We compare the modeling results with the in situ observations: OMNI data for L1, MESSENGER, Venus Express and Ulysses, all together covering 0.3 - 1.8 AU. We assess the capabilities of these models in capturing quasi-steady solar wind features including sector boundaries and the fast-slow stream interaction regions. For this particular period, we derive the strengths, weaknesses, and discrepancies from observations for each model, and discuss the effect of photospheric magnetograms from different observatories, such as GONG and SOLIS magnetograms from National Solar Observatory (NSO) and magnetograms from Mount Wilson Observatory (MWO).

  3. A Solar Axion Search Using a Decommissioned LHC Test Magnet

    CERN Multimedia

    Raffelt, G; Geralis, T; Lozza, V; Christensen, F E; Jakobsen, A C; Neff, S H; Dafni, T; Carmona martinez, J M; Giomataris, I; Krcmar, M; Vafeiadis, T; Bayirli, A; Luzon marco, G M; Lakic, B; Solanki, S K; Ozbey, A; Davenport, M; Funk, W; Desch, K K; Laurent, J; Villar, J A; Jakovcic, K; Eleftheriadis, C; Cantatore, G; Diago ortega, A; Gracia garza, J; Ortega ruiz, I; Papaevangelou, T; Zioutas, K; Gardikiotis, A; Hasinoff, M D; Hoffmann, D; Iguaz gutierrez, F J; Castel pablo, J F; Gninenko, S; Ferrer ribas, E; Liolios, A; Anastasopoulos, V; Kaminski, J; Garcia irastorza, I; Ruiz choliz, E; Krieger, C; Lutz, G; Fanourakis, G; Ruz armendariz, J; Vogel, J K; Pivovaroff, M J

    2002-01-01

    Previous solar axion searches have been carried out in Brookhaven (1990) and in Tokyo (2000- ), tracking the Sun with a dipole magnet. QCD inspired axions should be produced after the Big Bang, being thus candidates for the dark matter. The Sun is a very useful source of weakly interacting particles for fundamental research. Axions can be produced also in the Sun's core through the scattering of thermal photons in the Coulomb field of electric charges (Primakoff effect). In a transverse magnetic field the Primakoff effect can work in reverse, coherently converting the solar axions or other axion-like particles (ALPS) back into X-ray photons in the keV range. The conversion efficiency increases with $(B⋅L)^2$. In the CAST experiment an LHC prototype dipole magnet (B = 9 T and L = 10 m) with straight beam pipes provides a conversion efficiency exceeding that of the two earlier solar axion telescopes by almost a factor of 100. This magnet is mounted on a moving platform and coupled to both gas filled and soli...

  4. Effect of Polarimetric Noise on the Estimation of Twist and Magnetic Energy of Force-Free Fields

    CERN Document Server

    Tiwari, Sanjiv Kumar; Gosain, Sanjay; Joshi, Jayant

    2009-01-01

    The force-free parameter $\\alpha$, also known as helicity parameter or twist parameter, bears the same sign as the magnetic helicity under some restrictive conditions. The single global value of $\\alpha$ for a whole active region gives the degree of twist per unit axial length. We investigate the effect of polarimetric noise on the calculation of global $\\alpha$ value and magnetic energy of an analytical bipole. The analytical bipole has been generated using the force-free field approximation with a known value of constant $\\alpha$ and magnetic energy. The magnetic parameters obtained from the analytical bipole are used to generate Stokes profiles from the Unno-Rachkovsky solutions for polarized radiative transfer equations. Then we add random noise of the order of 10$^{-3}$ of the continuum intensity (I$_{c}$) in these profiles to simulate the real profiles obtained by modern spectropolarimeters like Hinode (SOT/SP), SVM (USO), ASP, DLSP, POLIS, SOLIS etc. These noisy profiles are then inverted using a Milne...

  5. The dazed and confused identity of Agassiz’s land tortoise, Gopherus agassizii (Testudines, Testudinidae) with the description of a new species, and its consequences for conservation

    Science.gov (United States)

    Murphy, Robert W.; Berry, Kristin H.; Edwards, Taylor; Leviton, Alan E.; Lathrop, Amy; Riedle, J. Daren

    2011-01-01

    Abstract We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii (Cooper). The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of Gopherus lepidocephalus (Ottley & Velázques Solis. 1989) from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA, and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because Gopherus lepidocephalus is from the Mojavian population, no names are available for the Sonoran species. Thus, a new species, Gopherus morafkai sp. n., is named and this action reduces the distribution of Gopherus agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of Gopherus agassizii, which may deserve a higher level of protection. PMID:21976992

  6. The dazed and confused identity of Agassiz’s land tortoise, Gopherus agassizii (Testudines: Testudinidae with the description of a new species and its consequences for conservation

    Directory of Open Access Journals (Sweden)

    Robert Murphy

    2011-06-01

    Full Text Available We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii Cooper. The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of G. lepidocephalus Ottley et Velázques Solis, 1989 from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because G. lepidocephalus is from the Mojavian population no names are available for the Sonoran species. Thus, a new species, Gopherus morafkai sp. n., is named and this action reduces the distribution of G. agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of G. agassizii, which may deserve a higher level of protection.

  7. The dazed and confused identity of Agassiz's land tortoise, Gopherus agassizii (Testudines, Testudinidae) with the description of a new species, and its consequences for conservation.

    Science.gov (United States)

    Murphy, Robert W; Berry, Kristin H; Edwards, Taylor; Leviton, Alan E; Lathrop, Amy; Riedle, J Daren

    2011-01-01

    We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii (Cooper). The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of Gopherus lepidocephalus (Ottley & Velázques Solis. 1989) from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA, and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because Gopherus lepidocephalus is from the Mojavian population, no names are available for the Sonoran species. Thus, a new species, Gopherus morafkaisp. n., is named and this action reduces the distribution of Gopherus agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of Gopherus agassizii, which may deserve a higher level of protection.

  8. Solar magnetic activity cycles, coronal potential field models and eruption rates

    CERN Document Server

    Petrie, G J D

    2013-01-01

    We study the evolution of the observed photospheric magnetic field and the modeled global coronal magnetic field during the past 3 1/2 solar activity cycles observed since the mid-1970s. We use synoptic magnetograms and extrapolated potential-field models based on longitudinal full-disk photospheric magnetograms from the NSO's three magnetographs at Kitt Peak, the Synoptic Optical Long-term Investigations of the Sun (SOLIS) vector spectro-magnetograph (VSM), the spectro-magnetograph and the 512-channel magnetograph instruments, and from the U. Stanford's Wilcox Solar Observatory. The associated multipole field components are used to study the dominant length scales and symmetries of the coronal field. Polar field changes are found to be well correlated with active fields over most of the period studied, except between 2003-6 when the active fields did not produce significant polar field changes. Of the axisymmetric multipoles, only the dipole and octupole follow the poles whereas the higher orders follow the ...

  9. Systematic functional analysis and application of a cold-active serine protease from a novel Chryseobacterium sp.

    Science.gov (United States)

    Mageswari, Anbazhagan; Subramanian, Parthiban; Chandrasekaran, Suganthi; Karthikeyan, Sivashanmugam; Gothandam, Kodiveri Muthukaliannan

    2017-02-15

    Psychrotolerant bacteria isolated from natural and artificially cold environments were screened for synthesis of cold-active protease. The strain IMDY showing the highest protease production at 5°C was selected and phylogenetic analysis revealed that IMDY as novel bacterium with Chryseobacterium soli(T) as its nearest neighbor. Classical optimization enhanced the protease production from 18U/mg to 26U/mg and the enzyme was found to be active at low temperature, activity enhanced by CaCl2, inhibited by PMSF, stable against NaCl, and its activity retained in the presence of surfactants, organic solvents and detergents. On testing, the meat tenderization, myofibril fragmentation, pH, and TBA values were favorable in IMDY-protease treated meat compared to control. SDS profiling and SEM analysis also showed tenderization in meat samples. Hence, this study proposes to consider the cold-active protease from Chryseobacterium sp. IMDY as a pertinent candidate to develop potential applications in food processing industry. PMID:27664603

  10. Forenzika elektronske pošte

    Directory of Open Access Journals (Sweden)

    Milorad S. Markagić

    2013-10-01

    podataka, pa ih je jednostavnije analizirati i generisati izveštaj. Analiza elektronske pošte zasnovane na internet serverima Za personalnu komunikaciju često  se koriste Web servisi za elektronsku poštu: Google, Yahoo, Hotmail, gmail.... Ovi se servisi koriste bez upotrebe softvera klijenata elektronske pošte i sačinjavaju klijent-server sistem. Web mail sistem predaje elektronsku poruku koristeći SMTP protokol i koristeći POP ili IMAP protokole. Web pošta se ne čuva se na lokalnom računaru, osim ako to korisnik izričito ne zahteva, već ista ostaje memorisana na serveru. Pristup nalogu elektronske pošte na serveru ili prikupljanje podataka o korisničkom nalogu od strane pružaoca usluga umnogome smanjuje količinu posla forenzičkom istražiocu. Ako pristup nije dozvoljen, do web mail poruka može se doći pregledom radne i keš memorije. Kada korisnik proverava poruke, ili sastavlja novu, operativni sistem sačuva podatke sa ekrana na hard disku, pogotovo ako korisniku treba više  vreme da sastavi poruku. Privremeni dokumenti Operativni sistemi privremene dokumente kreiraju za aplikacije koje šalju i primaju podatke preko mreže. Ti se podaci prvo skladište u radnoj memoriji, a kada se ista popuni, operativni sistem pomera podatke niže na listu prioriteta podataka koji su potrebni aplikacijama i zapisuje ih na tvrdi disk. Ne postoji posebno i jedinstveno područje gde se čuvaju privremeni dokumenti jer neke aplikacije stvaraju dodatne privremene datoteke uz one što ih stvara operativni sistem. Aplikacije koje nemaju mogućnost privremenog čuvanja podataka, prepuštaju operativnom sistemu da ih memoriše koristeći swap datoteke ili virtualnu memoriju. Kada aplikaciji zatreba privremeno sačuvani podatak, operativni sistem ga šalje aplikaciji i briše sa hard diska. Iako se swap datoteke brišu, do podataka je ipak moguće doći, jer još uvek fizički postoje na disku Instant poruke U većim organizacijama komunikacija sa korisnicima se

  11. Assessment of the efficacy of first-line antimalarial drugs after 5 years of deployment by the National Malaria Control Programme in Côte d'Ivoire

    Directory of Open Access Journals (Sweden)

    Offianan AT

    2011-11-01

    Full Text Available Andre T Offianan1, Serge B Assi2, Aristide MA Coulibaly1, Landry T N'guessan1, Aristide A Ako1, Florence K Kadjo2, Moïse K San2, Louis K Penali2 1Malariology Department, Institut Pasteur de Côte d'Ivoire, Abidjan, Côte d'Ivoire; 2National Malaria Control Programme, Abidjan, Côte d'Ivoire Background: The emergence of artemisinin resistance has raised concerns that the most potent antimalarial drug may be under threat. Artesunate + amodiaquine (ASAQ and artemether-lumefantrine (AL are respectively the first- and second-line treatments for uncomplicated falciparum malaria in Côte d'Ivoire. A comparison of the efficacy and safety of these two drug combinations was necessary to make evidence-based drug treatment policies. Methods: In an open-label, non inferiority, randomized, controlled clinical trial, children aged 6–59 months were randomized to receive ASAQ or AL. Both drug regimens were given for 3 days, and follow-up was for 28 days. The primary endpoint was the 28-day cure rates and was defined as proportion of patients with polymerase chain reaction (PCR-corrected cure rate after 28 days of follow-up. Findings: A total of 251 patients who were attending the Ayame and Dabakala hospitals and presenting with symptomatic acute uncomplicated falciparum malaria were randomized to receive ASAQ (128 and AL (123. The intention-to-treat analysis showed effectiveness rates of 94.5% and 93.5% for ASAQ and AL, respectively on day 28. After adjustment for PCR results, these rates were 96.1% and 96.8%, respectively. On day 28, the per-protocol analysis showed effectiveness rates of 98.4% and 96.6% for ASAQ and AL, respectively. After adjustment by PCR for reinfection, these rates were 100% for each drug, and both regimens were well tolerated. Conclusion: ASAQ and AL remain efficacious treatments of uncomplicated falciparum malaria in Ivorian children 5 years after adoption. The efficacy of ASAQ and AL in Côte d'Ivoire requires, therefore, continuous

  12. Demethylase Inhibitor Fungicide Resistance in Pyrenophora teres f. sp. teres Associated with Target Site Modification and Inducible Overexpression of Cyp51.

    Science.gov (United States)

    Mair, Wesley J; Deng, Weiwei; Mullins, Jonathan G L; West, Samuel; Wang, Penghao; Besharat, Naghmeh; Ellwood, Simon R; Oliver, Richard P; Lopez-Ruiz, Francisco J

    2016-01-01

    Pyrenophora teres f. sp. teres is the cause of net form of net blotch (NFNB), an economically important foliar disease in barley (Hordeum vulgare). Net and spot forms of net blotch are widely controlled using site-specific systemic fungicides. Although resistance to succinate dehydrogenase inhibitors and quinone outside inhibitors has been addressed before in net blotches, mechanisms controlling demethylation inhibitor resistance have not yet been reported at the molecular level. Here we report the isolation of strains of NFNB in Australia since 2013 resistant to a range of demethylase inhibitor fungicides. Cyp51A:KO103-A1, an allele with the mutation F489L, corresponding to the archetype F495I in Aspergillus fumigatus, was only present in resistant strains and was correlated with resistance factors to various demethylase inhibitors ranging from 1.1 for epoxiconazole to 31.7 for prochloraz. Structural in silico modeling of the sensitive and resistant CYP51A proteins docked with different demethylase inhibitor fungicides showed how the interaction of F489L within the heme cavity produced a localized constriction of the region adjacent to the docking site that is predicted to result in lower binding affinities. Resistant strains also displayed enhanced induced expression of the two Cyp51A paralogs and of Cyp51B genes. While Cyp51B was found to be constitutively expressed in the absence of fungicide, Cyp51A was only detected at extremely low levels. Under fungicide induction, expression of Cyp51B, Cyp51A2, and Cyp51A1 was shown to be 1.6-, 3,- and 5.3-fold higher, respectively in the resistant isolate compared to the wild type. These increased levels of expression were not supported by changes in the promoters of any of the three genes. The implications of these findings on demethylase inhibitor activity will require current net blotch management strategies to be reconsidered in order to avoid the development of further resistance and preserve the lifespan of

  13. Legislation of Renewable Energy Sources In Slovakia / Legislatívna Úprava Obnoviteľných Zdrojov Energie Na Slovensku

    Directory of Open Access Journals (Sweden)

    Marišová Eleonóra

    2015-12-01

    Full Text Available Pestovanie energetickych rastlin a drevin na poľnohospodarskej pode a ďalšie využitie ich energie predstavuje zasadny vyznam pre realizaciu dlhodobej strategie Slovenska v oblasti ziskavania a využivania obnoviteľnych zdrojov energie (OZE. Energeticke rastliny spĺňaju ciele strategie Europa 2020 a prispievaju k diverzifi kacii energetickych zdrojov. Prispevok vychadza z pravnych predpisov EU a narodnej legislativy upravujucej OZE. Analyzovane su Smernica 2009/28/ES Europskeho parlamentu a Rady z 23. aprila 2009 o podpore využivania energie z obnoviteľnych zdrojov a o zmene a naslednom zrušeni smernic 2001/77/ES a 2003/30/ES. Tema obnoviteľnych zdrojov energie je integrovana do Programu rozvoja vidieka 2014 - 2020. Konkretne Akčny plan pre biomasu 2008-2013, Strategia vyššieho využitia obnoviteľnych zdrojov energie na Slovensku a Strategia energetickej bezpečnosti Slovenska bola prijata až do roku 2030. Trvalo udržateľne využivanie poľnohospodarskej pody, jeho riadenie a využivanie, ako aj ochrana jeho kvality a funkcie su upravene zakonom č 220/2004 Zb. o ochrane a využivani poľnohospodarskej pody a ktorym sa meni zakon č. 245/2003 Zb., o integrovanej prevencii a kontrole znečisťovania životneho prostredia a o zmene niektorych zakonov v zneni neskoršich predpisov, ktory nadobudol učinnosť 1. maja 2004. Zakon č. 57/2013 Zb., ktory je platny od 1. aprila 2013, stanovuje zasady a postup zriaďovania plantaži rychlorastucich drevin na poľnohospodarskej pode. Slovenska pravna uprava zaviedla register rychlo rastucich drevin na každom okresnom urade (72, na pozemkovom a lesnom odbore na Slovensku. Prieskum tychto registrov ukazuje, že tato pravna uprava podporuje poľnohospodarov, aby začali použivať marginalne pody pre rychlo rastuce dreviny.

  14. Demethylase Inhibitor Fungicide Resistance in Pyrenophora teres f. sp. teres Associated with Target Site Modification and Inducible Overexpression of Cyp51.

    Science.gov (United States)

    Mair, Wesley J; Deng, Weiwei; Mullins, Jonathan G L; West, Samuel; Wang, Penghao; Besharat, Naghmeh; Ellwood, Simon R; Oliver, Richard P; Lopez-Ruiz, Francisco J

    2016-01-01

    Pyrenophora teres f. sp. teres is the cause of net form of net blotch (NFNB), an economically important foliar disease in barley (Hordeum vulgare). Net and spot forms of net blotch are widely controlled using site-specific systemic fungicides. Although resistance to succinate dehydrogenase inhibitors and quinone outside inhibitors has been addressed before in net blotches, mechanisms controlling demethylation inhibitor resistance have not yet been reported at the molecular level. Here we report the isolation of strains of NFNB in Australia since 2013 resistant to a range of demethylase inhibitor fungicides. Cyp51A:KO103-A1, an allele with the mutation F489L, corresponding to the archetype F495I in Aspergillus fumigatus, was only present in resistant strains and was correlated with resistance factors to various demethylase inhibitors ranging from 1.1 for epoxiconazole to 31.7 for prochloraz. Structural in silico modeling of the sensitive and resistant CYP51A proteins docked with different demethylase inhibitor fungicides showed how the interaction of F489L within the heme cavity produced a localized constriction of the region adjacent to the docking site that is predicted to result in lower binding affinities. Resistant strains also displayed enhanced induced expression of the two Cyp51A paralogs and of Cyp51B genes. While Cyp51B was found to be constitutively expressed in the absence of fungicide, Cyp51A was only detected at extremely low levels. Under fungicide induction, expression of Cyp51B, Cyp51A2, and Cyp51A1 was shown to be 1.6-, 3,- and 5.3-fold higher, respectively in the resistant isolate compared to the wild type. These increased levels of expression were not supported by changes in the promoters of any of the three genes. The implications of these findings on demethylase inhibitor activity will require current net blotch management strategies to be reconsidered in order to avoid the development of further resistance and preserve the lifespan of

  15. Survival rate and mortality of juvenile and immature eastern imperial eagles (Aquila heliaca from Bulgaria studied by satellite telemetry

    Directory of Open Access Journals (Sweden)

    Stoychev Stoycho

    2014-01-01

    Full Text Available U dlhožijúceho druhu A. heliaca trvá disperzia z miesta vyliahnutia niekolko rokov. Je to obdobie velmi dôležité pre prežitie a ochranu orlov. V tejto štúdii prezentujeme faktory mortality a prežívanie mladých a imaturných A. heliaca z Bulharska na základe štúdia satelitnou telemetriou. Celkom bolo v hniezdach v Bulharsku vysielackami GPS / Argos oznacených 20 mladých A. heliaca. Štrnást vtákov bolo sledovaných až do smrti vrátane nálezov kadáverov. Ide o prvé takéto štúdium prežívania mladých a imaturných jedincov A. heliaca. Prežívanie má hodnotu 59.1 % v prvom kalendárnom roku, 83.3 % v druhom a 80,0 % v tretom kalendárnom roku. Hlavným faktorom mortality sledovaných jedincov z bulharskej populácie je smrt v dôsledku zásahu elektrickým prúdom, ktorý spôsobil 59,0 % prípadov mortality. Dalšie identifikované hrozby sú zástrely, otravy a kolízie. Väcšina prípadov mortality sledovaných orlov nastala v Bulharsku (50% a v Turecku (43%. Preto je Turecko klúcovou krajinou pre ochranu bulharskej populácie A. heliaca pocas jeho disperzie. Orly z Bulharska sa rozptylujú aj dalej na juh, do Sudánu a do Saudskej Arábie. Preto je potrebné ochranárske úsilie s cielom zníženia mortality tak vo vnútri ako aj mimo Bulharska. Medzinárodná spolupráca a výmena skúseností by preto mala byt súcastou každej stratégie ochrany a plánov zameraných na ochranu orla královského.

  16. Conservation measures undertaken to improve the population status of eastern imperial eagle (Aquila heliaca in Bulgaria

    Directory of Open Access Journals (Sweden)

    Demerdzhiev Dimitar

    2014-01-01

    Full Text Available Štúdia prezentuje výsledky ochranárskych opatrení a ich vplyv na populáciu orla královského v Bulharsku. Druh sa vyskytuje v juhovýchodnej casti krajiny, v centrálnom Bulharsku je známy len jeden pár. V období 2009-201 3 bolo obsadených 28 rôznych hniezdnych teritórií. Populácia narástla o 20 %, pocet párov s hniezdnymi teritóriami sa zvýšil z 20 v roku 2009 na 24 v roku 201 3. Stredné hodnoty parametrov hniezdenia orlov královských boli v období 2009-201 3 nasledovné: produktivita (P 1 ,03 ± 0,1 8; hniezdna úspešnost (BS 1 ,1 8±0,21 , úspešnost vyletenia mládat (FS 1 .68±0,1 6. Rocná miera prežitia hniezdiacich jedincov bola 0,795-0,932. Najdôležitejším faktorom, spôsobujúcim mortalitu mladých orlov boli usmrtenia po zásahu elektrickým prúdom spolocne s nelegálnymi odstrelmi a otravami. Strážené páry mali vyššiu hniezdnu úspešnost (1 ,32±0,88 ako nestrážené páry (1 ,06±0,91 . Celkom bolo obsadených vtákmi rôznych druhov 39 % umelých hniezd inštalovaných v období 2009-201 3. V období 2008-201 3 bolo celkom 23 mladých orlov královských oznacených satelitnými vysielackami s GPS jednotkou. V teritóriách siedmich párov bolo izolovaných 608 nebezpecných stlpov elektrického vedenia. V jesennom a zimnom období (október až marec sa raz za týžden realizovalo prikrmovanie 1 4 párov orlov. Celkom sa zrealizovalo 483 prikrmovaní, na 450 z nich bolo súcastou sledovanie efektivity tejto cinnosti.

  17. Distribution, abundance, breeding parameters, threats and prey preferences of the eastern imperial eagle (Aquila heliaca in European Turkey

    Directory of Open Access Journals (Sweden)

    Demerdzhiev Dimitar

    2014-01-01

    Full Text Available V období 2008-201 3 bolo orlami královskými obsadených 32 rôznych hniezdnych teritórií. Tieto územia sa nachádzajú najmä v dvoch oblastiach: pohorie Derventa/Yıldız a územie na sever od Marmarského mora. Najbližšia vzdialenost medzi hniezdiskami bola 4,2 km. Stredná vzdialenost medzi pármi bola 1 0,44 ± 2,95 km. Priemerná hodnota hniezdnej úspešnosti v období 2008-201 3 vrátane 1 07 prípadov inkubácii bola 1 ,01±0,1 0. Priemerná hniezdna úspešnost vtákov v Marmarskom regióne (1 ,05±0,78 bola vyššia ako v oblasti pohorí Derventa/Yıldız (0,91±0,84. Predkladaná štúdia potravy orla královského bola založená na 582 zvyškoch koristi, obsahujúcej minimálne 70 taxónov. Cicavce boli najrozšírenejšie skupinou (46.5 %, nasledované vtákmi (32,4 % a plazmi (20,3 %. Najcastejšou koristou bol jež bledý (Erinaceus roumanicus a dva druhy korytnaciek - korytnacka zelenkastá (Eurotestudo hermanni a korytnacka grécka (Testudo graeca, ktoré dohromady predstavovali celkom 1 4,4 % z ulovenej koristi. Potravné spektrum sledovaných párov obsahoval tieto druhy koristi: cajka žltonohá (Larus michahellis, sysel pasienkový (Spermophilus citellus, domáca sliepka (Gallus gallus f. domestica a bocian biely (Ciconia ciconia. Tieto druhy dohromady tvorili 67,8 % z ulovenej koristi. Bol preukázaný štatisticky významný rozdiel (Z=2,52, p=0,01 v preferencii potravy orlov královských v oboch študovaných oblastiach.

  18. Genome sequencing of methanogenic Archaea Methanosarcina mazei TUC01 strain isolated from an Amazonian Flooded Area

    Science.gov (United States)

    Baraúna, R. A.; Graças, D. A.; Ramos, R. T.; Carneiro, A. R.; Lopes, T. S.; Lima, A. R.; Zahlouth, R. L.; Pellizari, V. H.; Silva, A.

    2013-05-01

    Methanosarcina mazei is a strictly anaerobic methanogen from the Methanosarcinales order. This species is known for its broad catabolic range among methanogens and is widespread throughout diverse environments. The draft genome of a strain cultivated from the sediment of the Tucuruí hydroelectric power station, the fourth largest hydroelectric dam in the world, is described here. Approximately 80% of methane is produced by biogenic sources, such as methanogenic archaea from M. mazei species. Although the methanogenesis pathway is well known, some aspects of the core genome, genome evolution and shared genes are still unclear. A sediment sample from the Tucuruí hydropower station reservoir was inoculated in mineral media supplemented with acetate and methanol. This media was maintained in an H2:CO2 (80:20) atmosphere to enrich and cultivate M. mazei. The enrichment was conducted at 30°C under standard anaerobic conditions. After several molecular and cellular analyses, total DNA was extracted from a non-pure culture of M. mazei, amplified using phi29 DNA polymerase (BioLabs) and finally used as a source template for genome sequencing. The draft genome was obtained after two rounds of sequencing. First, the genome was sequenced using a SOLiD System V3 with a mate-paired library, which yielded 24,405,103 and 24,399,268 reads (50 bp) for the R3 and F3 tags, respectively. The second round of sequencing was performed using the SOLiD 5500 XL platform with a mate-paired library, resulting in a total of 113,588,848 reads (60 bp) for each tag (F3 and R3). All reads obtained by this procedure were filtered using Quality Assessment software, whereby reads with an average quality score below Phred 20 were removed. Velvet and Edena were used to assemble the reads, and Simplifier was used to remove the redundant sequences. After this, a total of 16,811 contigs were obtained. M. mazei GO1 (AE008384) genome was used to map the contigs and generate the scaffolds. We used the

  19. 五倍子牙膏对釉质矿化作用的影响%Mineralization Effect of Galla Chinensis Toothpaste on Enamel in Vitro.

    Institute of Scientific and Technical Information of China (English)

    陈菲菲; 汪大照; 张向宇; 李金娥; 郭卯丁

    2011-01-01

    目的:评价五倍子牙膏抑制釉质脱矿和促进人工早期釉质龋再矿化的作用.方法:检测五倍子牙膏处理离体牙前后乳酸盐缓冲液中磷离子浓度的变化,计算出牙釉质溶解度降低率(enamel soly rate,ESR),评价五倍子牙膏抑制釉质脱矿作用;制作人工龋釉质块模型,体外药物pH循环,检测五倍子牙膏处理前后釉质表面显微硬度恢复率(enamel micro-hardness recovery,EMHR),扫描电镜观察再矿化后的釉质表面结构,评价五倍子牙膏对釉质再矿化的作用.结果:五倍子牙膏组的牙釉质溶解度降低率与空白牙膏组比较差异有统计学意义(P<0.05);五倍子牙膏组釉质表面显微硬度恢复率与空白牙膏组比较差异无统计学意义;扫描电镜结果显示五倍子牙膏组与空白牙膏组釉质表面为较疏松蜂巢状孔隙结构.结论:五倍子牙膏具有抑制釉质脱矿作用.%Objective: To investigate the effect of Galla Chinensis toothpaste on anti-demineralization and remineralization of enamel in vitro.Methods: Acid buffer was used to treat the extracted teeth samples alternately.Then concentration of acid buffer and enamel soly rate (ESR) were measured before and after the treatment of Galla Chinensis toothpaste and control group toothpaste.After using chemical demineralization models to form artificial initial enamel lesion, the enamel blocks were subjected to a pH-cycling regime in vitro.Then enamel micro-hardness recovery (EMHR) was examined and the microstructure of enamel lesions was observed by scanning electron microscope (SEM).Results: The ESR of Galla Chinensis toothpaste group has statistical significance compared with blank toothpaste group (P<0.05) and the EMHR of Galla Chinensis toothpaste group has no significance compared with deionized water group; there are some loose honeycomb pore structure on the enamel surface of Galla Chinensis toothpaste group and blank toothpaste group.Conclusion: The Galla Chinensis

  20. Next generation sequencing technology and its application in cancer research%新一代测序技术及其在肿瘤研究中的应用

    Institute of Scientific and Technical Information of China (English)

    肖岚(综述)

    2013-01-01

    The next generation sequencing (NGS), also known as high-throughput sequencing technology, is a landmark of revolution in the history for DNA sequencing. NGS have adopted a new sequencing strategy-cyclic-array sequencing, which was divided into pyrosequencing (Roche/454), Solexa sequencing technology (Illumina), the polony sequencing technology (ABI/SOLiD), Polonator sequencing technology and HeliScope single-molecule sequencing technology. Because of its high-throughput, low cost and short time-consuming, NGS has the potential to replace traditional Sanger sequencing and to become the most important research tools in post-genomic era.Researchers could do more comprehensive and in-depth analysis with genome, transcriptome and proteome at cheaper price. NGS has broad application prospects in the field of cancer research. In addition to accomplishing the tumor genome sequencing quickly, it also provides us a fully and rapidly powerful digital tool for tumor transcriptome, epigenetic and DNA-protein interaction analysis. It is expecting to bring about revolutionary change in the areas of life science, medicine science and others.%新一代测序技术又称为高通量测序技术,是DNA测序史上一次划时代的革命。新一代测序技术都采用了一种新的测序策略--循环芯片测序法(cyclic-array sequencing),可分为焦磷酸测序技术(Roche/454)、Solexa测序技术(Illumina)、polony测序技术(ABI/SOLiD)。由于其高通量、成本低、耗时短而迅速取代传统的Sanger测序成为后基因组时代的重要研究手段,让研究者能以更低廉的价格,更全面、更深入地分析基因组、转录组及蛋白质之间交互作用组。新一代测序技术除了可以快速实现肿瘤基因组测序外,还是数字化全面快速剖析肿瘤转录组、表观遗传及DNA-蛋白质交互作用的有力工具,有望在生命科学、医学科学等领域带来革命性的变革。Researchers could do more

  1. Deformations of the Protection Shelf in the "Wapno" Salt Mine, Based on Model Studies

    Science.gov (United States)

    Kortas, Grzegorz; Maj, Agnieszka

    2014-12-01

    The catastrophic mine failure resulting from the inrush of water into the Wapno Salt Mine was probably caused by a fracture in the roof protection shelf. The purpose of the present study was to apply the method of the homogenization of the multi-level room-and-pillar structure in the Wapno Salt Mine for 3D+t modelling of the elastic-viscous medium to determine the distribution of stress and strain, and, on that basis, to estimate the geomechanical conditions existing in the roof shelf. This paper presents briefly the spatial development of the salt mine's structure and the results of the surveying measurements carried out during the salt mine's operation and after the mine was flooded. Those results constituted a basis for the verification of the introduced homogenization parameters, i.e. the time-dependent changes of the elasticity modulus and the susceptibility to creep at particular salt mine's levels. A simulated process demonstrated the development of positive values of principle stresses, increasing with time, and of omnidirectional tensile strains. In such conditions, the cracking of the protection shelf body could proceed, also with opening of water flow paths from the dome cap into the salt mine's workings. This paper presents a new research method, the results of its application, and the obtained distribution of stresses and strains that can be useful for the assessment of water hazard in other salt mines. Katastrofa górnicza wywołana wdarciem wody do kopalni soli w Wapnie spowodowana była prawdopodobnie pęknięciem ochronnej półki stropowej. Celem tej pracy było zastosowanie metody homogenizacji struktury komorowo-filarowej wielopoziomowej kopalni Wapno do modelowania ośrodka sprężysto-lepkiego 3D+t dla określenia rozkładu naprężeń i odkształceń oraz na tej podstawie oszacowania warunków geomechanicznych w półce stropowej. W pracy przedstawiono krótko rozwój przestrzenny struktury kopalni oraz wyniki przeprowadzonych pomiar

  2. Annotation-based genome-wide SNP discovery in the large and complex Aegilops tauschii genome using next-generation sequencing without a reference genome sequence

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    Luo Ming-Cheng

    2011-01-01

    Full Text Available Abstract Background Many plants have large and complex genomes with an abundance of repeated sequences. Many plants are also polyploid. Both of these attributes typify the genome architecture in the tribe Triticeae, whose members include economically important wheat, rye and barley. Large genome sizes, an abundance of repeated sequences, and polyploidy present challenges to genome-wide SNP discovery using next-generation sequencing (NGS of total genomic DNA by making alignment and clustering of short reads generated by the NGS platforms difficult, particularly in the absence of a reference genome sequence. Results An annotation-based, genome-wide SNP discovery pipeline is reported using NGS data for large and complex genomes without a reference genome sequence. Roche 454 shotgun reads with low genome coverage of one genotype are annotated in order to distinguish single-copy sequences and repeat junctions from repetitive sequences and sequences shared by paralogous genes. Multiple genome equivalents of shotgun reads of another genotype generated with SOLiD or Solexa are then mapped to the annotated Roche 454 reads to identify putative SNPs. A pipeline program package, AGSNP, was developed and used for genome-wide SNP discovery in Aegilops tauschii-the diploid source of the wheat D genome, and with a genome size of 4.02 Gb, of which 90% is repetitive sequences. Genomic DNA of Ae. tauschii accession AL8/78 was sequenced with the Roche 454 NGS platform. Genomic DNA and cDNA of Ae. tauschii accession AS75 was sequenced primarily with SOLiD, although some Solexa and Roche 454 genomic sequences were also generated. A total of 195,631 putative SNPs were discovered in gene sequences, 155,580 putative SNPs were discovered in uncharacterized single-copy regions, and another 145,907 putative SNPs were discovered in repeat junctions. These SNPs were dispersed across the entire Ae. tauschii genome. To assess the false positive SNP discovery rate, DNA

  3. The complete genomic sequence of pepper yellow leaf curl virus (PYLCV) and its implications for our understanding of evolution dynamics in the genus polerovirus.

    Science.gov (United States)

    Dombrovsky, Aviv; Glanz, Eyal; Lachman, Oded; Sela, Noa; Doron-Faigenboim, Adi; Antignus, Yehezkel

    2013-01-01

    We determined the complete sequence and organization of the genome of a putative member of the genus Polerovirus tentatively named Pepper yellow leaf curl virus (PYLCV). PYLCV has a wider host range than Tobacco vein-distorting virus (TVDV) and has a close serological relationship with Cucurbit aphid-borne yellows virus (CABYV) (both poleroviruses). The extracted viral RNA was subjected to SOLiD next-generation sequence analysis and used as a template for reverse transcription synthesis, which was followed by PCR amplification. The ssRNA genome of PYLCV includes 6,028 nucleotides encoding six open reading frames (ORFs), which is typical of the genus Polerovirus. Comparisons of the deduced amino acid sequences of the PYLCV ORFs 2-4 and ORF5, indicate that there are high levels of similarity between these sequences to ORFs 2-4 of TVDV (84-93%) and to ORF5 of CABYV (87%). Both PYLCV and Pepper vein yellowing virus (PeVYV) contain sequences that point to a common ancestral polerovirus. The recombination breakpoint which is located at CABYV ORF3, which encodes the viral coat protein (CP), may explain the CABYV-like sequences found in the genomes of the pepper infecting viruses PYLCV and PeVYV. Two additional regions unique to PYLCV (PY1 and PY2) were identified between nucleotides 4,962 and 5,061 (ORF 5) and between positions 5,866 and 6,028 in the 3' NCR. Sequence analysis of the pepper-infecting PeVYV revealed three unique regions (Pe1-Pe3) with no similarity to other members of the genus Polerovirus. Genomic analyses of PYLCV and PeVYV suggest that the speciation of these viruses occurred through putative recombination event(s) between poleroviruses co-infecting a common host(s), resulting in the emergence of PYLCV, a novel pathogen with a wider host range. PMID:23936244

  4. Identification and validation of human papillomavirus encoded microRNAs.

    Directory of Open Access Journals (Sweden)

    Kui Qian

    Full Text Available We report here identification and validation of the first papillomavirus encoded microRNAs expressed in human cervical lesions and cell lines. We established small RNA libraries from ten human papillomavirus associated cervical lesions including cancer and two human papillomavirus harboring cell lines. These libraries were sequenced using SOLiD 4 technology. We used the sequencing data to predict putative viral microRNAs and discovered nine putative papillomavirus encoded microRNAs. Validation was performed for five candidates, four of which were successfully validated by qPCR from cervical tissue samples and cell lines: two were encoded by HPV 16, one by HPV 38 and one by HPV 68. The expression of HPV 16 microRNAs was further confirmed by in situ hybridization, and colocalization with p16INK4A was established. Prediction of cellular target genes of HPV 16 encoded microRNAs suggests that they may play a role in cell cycle, immune functions, cell adhesion and migration, development, and cancer. Two putative viral target sites for the two validated HPV 16 miRNAs were mapped to the E5 gene, one in the E1 gene, two in the L1 gene and one in the LCR region. This is the first report to show that papillomaviruses encode their own microRNA species. Importantly, microRNAs were found in libraries established from human cervical disease and carcinoma cell lines, and their expression was confirmed in additional tissue samples. To our knowledge, this is also the first paper to use in situ hybridization to show the expression of a viral microRNA in human tissue.

  5. Trasformazione urbana verso la mobilità dolce: il caso di Istanbul

    Directory of Open Access Journals (Sweden)

    Emilia Giovanna Trifiletti

    2008-12-01

    Full Text Available Nell’articolo si descrivono le recenti trasformazioni urbane della città di Istanbul in Turchia in relazione all’implementazione di aree pedonali e all’utilizzo di mobilità dolce. Il sistema trasportistico è stato ampliato ed integrato di recente ed in tempi molto brevi: nei soli ultimi due anni è stata potenziata la linea tranviaria, sono state inaugurate una funicolare ed una teleferica ed è stata realizzata una stazione di interscambio gomma-ferro, ponendo grande attenzione all’integrazione del trasporto su ferro e/o gomma con la mobilità pedonale. Allo stesso modo sono state implementate le linee marittime che collegano le diverse sponde della città ed integrate alle linee su ferro e su gomma. Lo sviluppo e la riqualificazione della città sono dunque stati guidati dalla trasformazione del sistema trasportistico che ha rappresentato il vero motore della riqualificazione urbana. L’antico quartiere di Sultanahmet è stato interamente riqualificato e collegato, attraverso la linea tranviaria veloce che lo attraversa interamente, al resto della città fino ai quartieri più periferici. Allo stesso modo nel quartiere di Beyoglu che, dopo l’indipendenza era caduto in totale rovina, ha riacquistato l’antica eleganza che lo connotava. Il viale dell’Indipendenza (antica Rue De Pera che attraversa tutto il quartiere è stato reso pedonale e con un tram vintage che percorre tutto il viale sono stati collegati il nodo di interscambio Taksim, dotato di metropolitana e funicolare, con il nodo di interscambio Tünel, dotato di tram veloce e trasporto su gomma. Nell’articolo si è inteso evidenziare come la riqualificazione urbana della città sia stata guidata da interventi integrati trasporto/territorio e come i risultati ottenuti, nel caso-studio, siano stati anche il frutto di politiche attente alla sicurezza sociale.

  6. Integrating sequencing technologies in personal genomics: optimal low cost reconstruction of structural variants.

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    Jiang Du

    2009-07-01

    Full Text Available The goal of human genome re-sequencing is obtaining an accurate assembly of an individual's genome. Recently, there has been great excitement in the development of many technologies for this (e.g. medium and short read sequencing from companies such as 454 and SOLiD, and high-density oligo-arrays from Affymetrix and NimbelGen, with even more expected to appear. The costs and sensitivities of these technologies differ considerably from each other. As an important goal of personal genomics is to reduce the cost of re-sequencing to an affordable point, it is worthwhile to consider optimally integrating technologies. Here, we build a simulation toolbox that will help us optimally combine different technologies for genome re-sequencing, especially in reconstructing large structural variants (SVs. SV reconstruction is considered the most challenging step in human genome re-sequencing. (It is sometimes even harder than de novo assembly of small genomes because of the duplications and repetitive sequences in the human genome. To this end, we formulate canonical problems that are representative of issues in reconstruction and are of small enough scale to be computationally tractable and simulatable. Using semi-realistic simulations, we show how we can combine different technologies to optimally solve the assembly at low cost. With mapability maps, our simulations efficiently handle the inhomogeneous repeat-containing structure of the human genome and the computational complexity of practical assembly algorithms. They quantitatively show how combining different read lengths is more cost-effective than using one length, how an optimal mixed sequencing strategy for reconstructing large novel SVs usually also gives accurate detection of SNPs/indels, how paired-end reads can improve reconstruction efficiency, and how adding in arrays is more efficient than just sequencing for disentangling some complex SVs. Our strategy should facilitate the sequencing of

  7. Biomechanical stability of a bioabsorbable self-retaining polylactic acid/nano-sized β-tricalcium phosphate cervical spine interbody fusion device in single-level anterior cervical discectomy and fusion sheep models

    Directory of Open Access Journals (Sweden)

    Cao L

    2012-11-01

    Full Text Available Lu Cao,1 Ping-Guo Duan,1 Xi-Lei Li,1 Feng-Lai Yuan,3 Ming-Dong Zhao,2 Wu Che,1 Hui-Ren Wang,1 Jian Dong11Department of Orthopedic Surgery, Zhongshan Hospital, State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China; 2Department of Orthopedic Surgery, Jinshan Hospital, Fudan University, Shanghai, China; 3Affiliated Third Hospital of Nantong University, Wuxi, ChinaPurpose: The aim of this study was to investigate the biomechanical stability provided by a novel, polylactic acid/nano-sized, β-tricalcium phosphate, bioabsorbable, self-retaining cervical fusion cage (BCFC.Methods: Quasistatic nonconstraining torques (maximum 1.5 NM induced flexion, extension, lateral bending (±1.5 NM, and axial rotation (±1.5 NM on 32 sheep cervical spines (C2–C5. The motion segment C3–C4 was first tested intact; the following groups were tested after complete discectomy: autologous tricortical iliac crest bone graft, Medtronic–Wego polyetheretherketone (PEEK cage, Solis PEEK cage, and BCFC. The autologous bone graft group was tested with an anterior plate. The mean range of motion (ROM was calculated from the load-displacement curves.Results: BCFC significantly decreased ROM in lateral bending and axial rotation compared to other implants, and no significant difference in ROM between two types of PEEK cages and BCFC could be observed in flexion and extension. Anterior cervical plate (ACP significantly decreased ROM in flexion and extension, but no significant difference in ROM between BCFC and bone graft plus ACP could be determined in lateral bending and axial rotation.Conclusion: The BCFC device showed better stability to autologous tricortical iliac crest bone graft and PEEK cages in single-level anterior cervical discectomy and fusion models and thus may be a potential alternative to the current PEEK cages.Keywords: biomechanics, cervical spine, cages, bioabsorbable, sheep

  8. Development of Prediction Model and Experimental Validation in Predicting the Curcumin Content of Turmeric (Curcuma longa L.)

    Science.gov (United States)

    Akbar, Abdul; Kuanar, Ananya; Joshi, Raj K.; Sandeep, I. S.; Mohanty, Sujata; Naik, Pradeep K.; Mishra, Antaryami; Nayak, Sanghamitra

    2016-01-01

    The drug yielding potential of turmeric (Curcuma longa L.) is largely due to the presence of phyto-constituent ‘curcumin.’ Curcumin has been found to possess a myriad of therapeutic activities ranging from anti-inflammatory to neuroprotective. Lack of requisite high curcumin containing genotypes and variation in the curcumin content of turmeric at different agro climatic regions are the major stumbling blocks in commercial production of turmeric. Curcumin content of turmeric is greatly influenced by environmental factors. Hence, a prediction model based on artificial neural network (ANN) was developed to map genome environment interaction basing on curcumin content, soli and climatic factors from different agroclimatic regions for prediction of maximum curcumin content at various sites to facilitate the selection of suitable region for commercial cultivation of turmeric. The ANN model was developed and tested using a data set of 119 generated by collecting samples from 8 different agroclimatic regions of Odisha. The curcumin content from these samples was measured that varied from 7.2% to 0.4%. The ANN model was trained with 11 parameters of soil and climatic factors as input and curcumin content as output. The results showed that feed-forward ANN model with 8 nodes (MLFN-8) was the most suitable one with R2 value of 0.91. Sensitivity analysis revealed that minimum relative humidity, altitude, soil nitrogen content and soil pH had greater effect on curcumin content. This ANN model has shown proven efficiency for predicting and optimizing the curcumin content at a specific site. PMID:27766103

  9. Discovery of posttranscriptional regulatory RNAs using next generation sequencing technologies.

    Science.gov (United States)

    Gelderman, Grant; Contreras, Lydia M

    2013-01-01

    Next generation sequencing (NGS) has revolutionized the way by which we engineer metabolism by radically altering the path to genome-wide inquiries. This is due to the fact that NGS approaches offer several powerful advantages over traditional methods that include the ability to fully sequence hundreds to thousands of genes in a single experiment and simultaneously detect homozygous and heterozygous deletions, alterations in gene copy number, insertions, translocations, and exome-wide substitutions that include "hot-spot mutations." This chapter describes the use of these technologies as a sequencing technique for transcriptome analysis and discovery of regulatory RNA elements in the context of three main platforms: Illumina HiSeq, 454 pyrosequencing, and SOLiD sequencing. Specifically, this chapter focuses on the use of Illumina HiSeq, since it is the most widely used platform for RNA discovery and transcriptome analysis. Regulatory RNAs have now been found in all branches of life. In bacteria, noncoding small RNAs (sRNAs) are involved in highly sophisticated regulatory circuits that include quorum sensing, carbon metabolism, stress responses, and virulence (Gorke and Vogel, Gene Dev 22:2914-2925, 2008; Gottesman, Trends Genet 21:399-404, 2005; Romby et al., Curr Opin Microbiol 9:229-236, 2006). Further characterization of the underlying regulation of gene expression remains poorly understood given that it is estimated that over 60% of all predicted genes remain hypothetical and the 5' and 3' untranslated regions are unknown for more than 90% of the genes (Siegel et al., Trends Parasitol 27:434-441, 2011). Importantly, manipulation of the posttranscriptional regulation that occurs at the level of RNA stability and export, trans-splicing, polyadenylation, protein translation, and protein stability via untranslated regions (Clayton, EMBO J 21:1881-1888, 2002; Haile and Papadopoulou, Curr Opin Microbiol 10:569-577, 2007) could be highly beneficial to metabolic

  10. Characterization of the Burkholderia thailandensis SOS response by using whole-transcriptome shotgun sequencing.

    Science.gov (United States)

    Ulrich, Ricky L; Deshazer, David; Kenny, Tara A; Ulrich, Melanie P; Moravusova, Anna; Opperman, Timothy; Bavari, Sina; Bowlin, Terry L; Moir, Donald T; Panchal, Rekha G

    2013-10-01

    The bacterial SOS response is a well-characterized regulatory network encoded by most prokaryotic bacterial species and is involved in DNA repair. In addition to nucleic acid repair, the SOS response is involved in pathogenicity, stress-induced mutagenesis, and the emergence and dissemination of antibiotic resistance. Using high-throughput sequencing technology (SOLiD RNA-Seq), we analyzed the Burkholderia thailandensis global SOS response to the fluoroquinolone antibiotic, ciprofloxacin (CIP), and the DNA-damaging chemical, mitomycin C (MMC). We demonstrate that a B. thailandensis recA mutant (RU0643) is ∼4-fold more sensitive to CIP in contrast to the parental strain B. thailandensis DW503. Our RNA-Seq results show that CIP and MMC treatment (P SOS response were induced and include lexA, uvrA, dnaE, dinB, recX, and recA. At the genome-wide level, we found an overall decrease in gene expression, especially for genes involved in amino acid and carbohydrate transport and metabolism, following both CIP and MMC exposure. Interestingly, we observed the upregulation of several genes involved in bacterial motility and enhanced transcription of a B. thailandensis genomic island encoding a Siphoviridae bacteriophage designated E264. Using B. thailandensis plaque assays and PCR with B. mallei ATCC 23344 as the host, we demonstrate that CIP and MMC exposure in B. thailandensis DW503 induces the transcription and translation of viable bacteriophage in a RecA-dependent manner. This is the first report of the SOS response in Burkholderia spp. to DNA-damaging agents. We have identified both common and unique adaptive responses of B. thailandensis to chemical stress and DNA damage.

  11. Agronomic Potential of Partially Acidulated Rock Phosphates in Acid Soils of Subtropical China

    Institute of Scientific and Technical Information of China (English)

    XIONGLI-MING; B.TRUONG; 等

    1995-01-01

    A glasshouse experiment was conducted to evaluate the agronomic potential of four partially acidulated rock phosphates(PARP) in three representative solis sampled from subtripical China.The PARPs were manufactured by attacking a moderately reactive phosphate rock either with sulfuric acid alone or with combination of sulfuric and phosphoric acids at 30 or 60 percent of acidulation.Shoot dry weight and P accumulation of six successive cuttings of ryegrass were used to compare the agronomic potential of these fertilizers with that of the raw rock phosphate(RP) and monocalcium phosphate (MCP).Results indicated that the effectiveness of various phosphates was determined both by the solubility of the phosphates and by the acidity and P-fixing capacity of the soils.The higher the watersoluble P contained,the better the effectiveness of the fertilizer was.Although plant P accumulation of PARP treatments was constantly lower than that of MCP treatment,some PARPs could still get a dry matter production similar to that of MCP treatment.PARP SP60,which was acidulated with a mixture of sulfuric acid and phosphoric acid at 60 percent of acidulation and contained the highest soluble,P,was as effective as MCP in terms of dry matter production on all the soils.S60 and C1 which were both acidulated with sulfuric acid with the former at 60 percent of acidulation and the latter at 30 percent but with a further addition of monoammonium phosphate,were more than 80 percent as efective as MCP,Raw RP also showed a reasonable effectiveness which increased with soil acidity.It was suggested from the study that some of these APRPs could be expected to have a comparable field performance as soluble P fertilizers in the acid soil regions.

  12. Differential expression patterns of conserved miRNAs and isomiRs during Atlantic halibut development

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    Bizuayehu Teshome T

    2012-01-01

    Full Text Available Abstract Background MicroRNAs (miRNAs play a major role in animal ontogenesis. Size variants of miRNAs, isomiRs, are observed along with the main miRNA types, but their origin and possible biological role are uncovered yet. Developmental profiles of miRNAs have been reported in few fish species only and, to our knowledge, differential expressions of isomiRs have not yet been shown during fish development. Atlantic halibut, Hippoglossus hippoglossus L., undergoes dramatic metamorphosis during early development from symmetrical pelagic larval stage to unsymmetrical flatfish. No data exist on role of miRNAs in halibut metamorphosis. Results miRNA profiling using SOLiD deep sequencing technology revealed a total of 199 conserved, one novel antisense, and one miRNA* mature form. Digital expression profiles of selected miRNAs were validated using reverse transcription quantitative PCR. We found developmental transition-specific miRNA expression. Expression of some miRNA* exceeded the guide strand miRNA. We revealed that nucleotide truncations and/or additions at the 3' end of mature miRNAs resulted in size variants showing differential expression patterns during the development in a number of miRNA families. We confirmed the presence of isomiRs by cloning and Sanger sequencing. Also, we found inverse relationship between expression levels of sense/antisense miRNAs during halibut development. Conclusion Developmental transitions during early development of Atlantic halibut are associated with expression of certain miRNA types. IsomiRs are abundant and often show differential expression during the development.

  13. Evaluation of biostimulation and Tween 80 addition for the bioremediation of long-term DDT-contaminated soil.

    Science.gov (United States)

    Betancur-Corredor, Bibiana; Pino, Nancy J; Cardona, Santiago; Peñuela, Gustavo A

    2015-02-01

    The bioremediation of a long-term contaminated soil through biostimulation and surfactant addition was evaluated. The concentrations of 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT) and its metabolites 1,1-dichloro-2,2-bis(4-chlorophenyl) ethane (DDD) and 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (DDE) were monitored during an 8-week remediation process. Physicochemical characterization of the treated soil was performed before and after the bioremediation process. The isolation and identification of predominant microorganisms during the remediation process were also carried out. The efficiency of detoxification was evaluated after each bioremediation protocol. Humidity and pH and the heterotrophic microorganism count were monitored weekly. The DDT concentration was reduced by 79% after 8 weeks via biostimulation with surfactant addition (B+S) and 94.3% via biostimulation alone (B). Likewise, the concentrations of the metabolites DDE and DDD were reduced to levels below the quantification limits. The microorganisms isolated during bioremediation were identified as Bacillus thuringiensis, Flavobacterium sp., Cuprivadius sp., Variovorax soli, Phenylobacterium sp. and Lysobacter sp., among others. Analysis with scanning electron microscopy (SEM) allowed visualization of the colonization patterns of soil particles. The toxicity of the soil before and after bioremediation was evaluated using Vibrio fischeri as a bioluminescent sensor. A decrease in the toxic potential of the soil was verified by the increase of the concentration/effect relationship EC50 to 26.9% and 27.2% for B+S and B, respectively, compared to 0.4% obtained for the soil before treatment and 2.5% by natural attenuation after 8 weeks of treatment.

  14. The complete genomic sequence of pepper yellow leaf curl virus (PYLCV and its implications for our understanding of evolution dynamics in the genus polerovirus.

    Directory of Open Access Journals (Sweden)

    Aviv Dombrovsky

    Full Text Available We determined the complete sequence and organization of the genome of a putative member of the genus Polerovirus tentatively named Pepper yellow leaf curl virus (PYLCV. PYLCV has a wider host range than Tobacco vein-distorting virus (TVDV and has a close serological relationship with Cucurbit aphid-borne yellows virus (CABYV (both poleroviruses. The extracted viral RNA was subjected to SOLiD next-generation sequence analysis and used as a template for reverse transcription synthesis, which was followed by PCR amplification. The ssRNA genome of PYLCV includes 6,028 nucleotides encoding six open reading frames (ORFs, which is typical of the genus Polerovirus. Comparisons of the deduced amino acid sequences of the PYLCV ORFs 2-4 and ORF5, indicate that there are high levels of similarity between these sequences to ORFs 2-4 of TVDV (84-93% and to ORF5 of CABYV (87%. Both PYLCV and Pepper vein yellowing virus (PeVYV contain sequences that point to a common ancestral polerovirus. The recombination breakpoint which is located at CABYV ORF3, which encodes the viral coat protein (CP, may explain the CABYV-like sequences found in the genomes of the pepper infecting viruses PYLCV and PeVYV. Two additional regions unique to PYLCV (PY1 and PY2 were identified between nucleotides 4,962 and 5,061 (ORF 5 and between positions 5,866 and 6,028 in the 3' NCR. Sequence analysis of the pepper-infecting PeVYV revealed three unique regions (Pe1-Pe3 with no similarity to other members of the genus Polerovirus. Genomic analyses of PYLCV and PeVYV suggest that the speciation of these viruses occurred through putative recombination event(s between poleroviruses co-infecting a common host(s, resulting in the emergence of PYLCV, a novel pathogen with a wider host range.

  15. Seven-year survival after pancreaticoduodenectomy for early recurrent renal cell carcinoma involving the duodenum A case report.

    Science.gov (United States)

    Cannistra', Marco; Ruggiero, Michele; Bonaiuto, Elisabetta; Vaccarisi, Sebastiano; Naso, Agostino; Grande, Raffaele; Nardo, Bruno

    2015-12-29

    Il cancro del rene rappresenta la 13a forma di tumore a livello mondiale e risulta incrementata, dal punto di vista epidemiologico, di circa il 2% annuo nelle ultime due decadi. Studi recenti hanno mostrato un numero complessivo di 65,150 nuovi casi di cancro renale diagnosticato nei paesi occidentali e circa 13.680 morti. Il carcinoma a cellule renali rappresenta approssimativamente il 90% di tutte le forme di cancro renale e presenta un tasso di mortalità di circa 4 soggetti ogni 100000 abitanti per anno. Il carcinoma a cellule renali ha, spesso, un particolare corteo sintomatologico caratterizzato da dolore addominale, masse addominali ed ematuria. Caratteristico è il fatto che circa il 25-30% dei pazienti, all’atto della diagnosi, presentano già metastasti a distanza; un ulteriore 30-50%, inoltre, svilupperanno metastasi nel corso della malattia, anche dopo molti anni dall’operazione chirurgica di nefrectomia radicale. Il Cancro del Rene a Cellule Chiare metastatizza in genere per via linfatica, ematogena, transperitoneale o per invasione diretta, ed i siti di metastasi più comuni sono il polmone ed i linfonodi, seguiti dalle ossa e dal fegato. Tra tutte le possibili localizzazioni ai segmenti addominali, il duodeno rappresenta la sede meno colpita: quando coinvolta, viene, in genere, invasa la regione periampollare ed il bulbo del duodeno. Tali localizzazioni danno segno di sè, spesso tardivamente, con sanguinamenti (ematemesi) o ostruzioni del tratto gastrointestinale superiore (vomito). Il nostro caso clinico descrive un carcinoma a cellule renali che si presentava, dopo soli 5 mesi dalla nefrectomia radicale destra, con sanguinamento del tratto gastrointestinale superiore e metastasi a carico del duodeno, e che è stato trattato chirurgicamente con duodenocefalopancreasectomia. L’intervento chirurgico, noto per essere altamente demolitivo ed associato ad elevata morbilità, applicato a recidiva precoce di cancro, ha garantito un ottimo risultato

  16. Ca II 854.2 nm Spectromagnetograms: A Powerful Chromospheric Diagnostic

    Science.gov (United States)

    Harvey, J. W.; Bertello, Luca; Branston, D.; Britanik, J.; Bulau, S.; Cole, L.; Gosain, Sanjay; Harker, Brian; Jones, Harrison P.; Marble, A.; Martinez Pillet, V.; Pevtsov, A.; Schramm, K.; Streander, Kim; Villegas, H.

    2016-05-01

    The transition from physical dominance by plasma flows in the photosphere to magnetic pressure in the solar chromosphere motivates as many diagnostic observations as possible across this important region. Among the few ground-accessible spectral lines formed within the chromosphere, the Ca II 854.2 nm line has the desirable properties of presence everywhere on the solar disk, Zeeman sensitivity, and narrow line width. Mapped observations of circular polarization within this line (spectromagnetograms) have been made at NSO infrequently since 1974, with regular daily full-disk observations starting in August 1996. Full-disk spectral observations of the complete Stokes polarization vector are now being made regularly since November 2015. It is not easy to estimate chromospheric magnetic field properties from the 854.2 nm line profile polarization. To provide rough quick-look vector field maps we found that the weak-field approximation provides a fair first estimate of the line-of-sight component but appears to be too simple to interpret the transverse magnetic field from frequently asymmetric, linearly-polarized line profiles. More realistic estimates of the chromospheric vector field, short of extremely lengthy, full 3D, non-local radiative transfer inversions, are being investigated. We briefly introduce recent instrumental modifications and observational characteristics, sample observations, and results concerning the expansion of the chromospheric field with increasing height, the presence of large areas of weak, nearly horizontal fields, and field estimates in plages, sunspots, flares, filaments, and filament channels. The Stokes spectra will be freely available to the community.This work utilizes SOLIS data obtained by the NSO Integrated Synoptic Program (NISP), managed by the National Solar Observatory, which is operated by the Association of Universities for Research in Astronomy (AURA), Inc. under a cooperative agreement with the National Science Foundation.

  17. Mucilaginibacter carri sp. nov., isolated from a car air conditioning system.

    Science.gov (United States)

    Kim, Dong-Uk; Lee, Hyosun; Kim, Hyun; Kim, Song-Gun; Park, So Yoon; Ka, Jong-Ok

    2016-04-01

    A Gram-stain-negative, pink bacterial strain, designated PR0008KT, was isolated from an automobile evaporator core in Korea. The cells were obligately aerobic and rod-shaped. The strain grew at 10-40 °C (optimum, 20 °C), at pH 5-8 (optimum, 7), and in the presence of 0-1.5% (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus Mucilaginibacter (93.4-97.0% 16S rRNA sequence similarities) and showed a high sequence similarity with Mucilaginibacter litoreus BR-18T, Mucilaginibacter lutimaris BR-3T and Mucilaginibacter soli R9-65T (97.0%, 96.9% and 96.9% 16S rRNA sequence similarity, respectively). It contained summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16 : 0, iso-C17:0 3-OH and C16:0 as the predominant fatty acids and MK-7 as the major menaquinone. The polar lipids were phosphatidylethanolamine, one unknown aminophospholipid, two unknown aminolipids and two unknown polar lipids. The DNA G+C content of this strain was 47.4 mol%. Based on the phenotypic, genotypic and chemotaxonomic data, strain PR0008KT represents a novel species in the genus Mucilaginibacter, for which the name Mucilaginibacter carri sp. nov. (=KACC 17938T=NBRC 111539T) is proposed.

  18. Photospheric and coronal magnetic fields in six magnetographs. I. Consistent evolution of the bashful ballerina

    Science.gov (United States)

    Virtanen, Ilpo; Mursula, Kalevi

    2016-06-01

    Aims: We study the long-term evolution of photospheric and coronal magnetic fields and the heliospheric current sheet (HCS), especially its north-south asymmetry. Special attention is paid to the reliability of the six data sets used in this study and to the consistency of the results based on these data sets. Methods: We use synoptic maps constructed from Wilcox Solar Observatory (WSO), Mount Wilson Observatory (MWO), Kitt Peak (KP), SOLIS, SOHO/MDI, and SDO/HMI measurements of the photospheric field and the potential field source surface (PFSS) model. Results: The six data sets depict a fairly similar long-term evolution of magnetic fields and the heliospheric current sheet, including polarity reversals and hemispheric asymmetry. However, there are time intervals of several years long, when first KP measurements in the 1970s and 1980s, and later WSO measurements in the 1990s and early 2000s, significantly deviate from the other simultaneous data sets, reflecting likely errors at these times. All of the six magnetographs agree on the southward shift of the heliospheric current sheet (the so-called bashful ballerina phenomenon) in the declining to minimum phase of the solar cycle during a few years of the five included cycles. We show that during solar cycles 20-22, the southward shift of the HCS is mainly due to the axial quadrupole term, reflecting the stronger magnetic field intensity at the southern pole during these times. During cycle 23 the asymmetry is less persistent and mainly due to higher harmonics than the quadrupole term. Currently, in the early declining phase of cycle 24, the HCS is also shifted southward and is mainly due to the axial quadrupole as for most earlier cycles. This further emphasizes the special character of the global solar field during cycle 23.

  19. The Turning and Evolution of the Recent Acceleration Universe

    Science.gov (United States)

    Zhang, Tianxi; Tan, A.

    2007-05-01

    The turning point and evolution characteristics of the universe are investigated through solving the Friedmann equation with a non-zero cosmological constant. Choosing the present-time Hubble constant, the radius of the present universe , and the density parameter in matter as three key parameters, we obtain the density parameter in dark energy, the cosmological constant, the mass of the universe, the turning point redshif, the age of the present universe, and the time-dependent expansion rate, velocity, radius, and acceleration parameter of the universe. It is shown that the turing point redshift is soly dependent of the density parameters in matter and dark energy. For the flat universe, it turned from past deceleration to recent acceleration when its size was 1/2 to 2/3 of the present size if the density parameter in matter is between 0.2 and 0.4. The expansion rate is very large at initial and decreases with time to approach the Hubble constant. The expansion velocity can be over the light speed in the early period, which decreases to the minimum at the turning point and then increases with time to approach the ratio of the present radius to the Hubble radius times the square root of the density parameter in dark energy. The solution of the time-dependent radius increases with time. The present time depends on the three key parameters. The universe with a larger present radius, smaller Hubble constant, or smaller density parameter in dark energy is elder. The universe with greater density parameter in dark energy accelerates faster recently. The open and closed universes can also be accelerated recently. The turning points and evolution characteristics among different types of the universe and different sets of key parameters are compared. This presentation will show the details, supported by NASA grant (NNG04GD59G).

  20. Bashful ballerina: Multi-instrument verification and recent behaviour

    Science.gov (United States)

    Mursula, Kalevi; Virtanen, Ilpo

    2016-07-01

    Heliospheric current sheet (HCS) is the continuation of the coronal magnetic equator into space, dividing the heliospheric magnetic field (HMF) into two sectors. Because of its wavy structure, the HCS is also called the ballerina skirt. Several recent studies have proven that the HCS is southward shifted during about three years in the solar declining to minimum phase. This persistent phenomenon, now called the Bashful ballerina, has been seen in geomagnetic indices since 1930s, OMNI data since 1960s, WSO data since mid-1970s and in Ulysses probe measurements during the fast latitude scans in 1994-1995 and 2007. Here we study the long-term evolution of photospheric and coronal magnetic fields and the heliospheric current sheet since 1975 using synoptic maps from six observatories (WSO, MWO, Kitt Peak, SOLIS, SOHO/MDI and SDO/HMI). All data sets depict a fairly similar long-term evolution of magnetic fields and the heliospheric current sheet, and agree on the southward shift of the heliospheric current sheet during all the five included cycles. We show that during solar cycles 20 -- 22, the southward shift of the HCS is due to the axial quadrupole term, reflecting the stronger magnetic field intensity at the southern pole during these times. During cycle 23 the asymmetry is less persistent and due to higher harmonics than the quadrupole term. Currently, in the early declining phase of cycle 24, the HCS is also shifted southward and is, again, due to the axial quadrupole, repeating the pattern of most previous cycles.

  1. Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis

    KAUST Repository

    Cui, Peng

    2012-06-08

    The global features of H3K4 and H3K27 trimethylations (H3K4me3 and H3K27me3) have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. However, the bivalent domains tend towards a “winner-takes-all” approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a “winner-takes-all” principle to regulate gene expression.

  2. Ago2 immunoprecipitation identifies predicted microRNAs in human embryonic stem cells and neural precursors.

    Directory of Open Access Journals (Sweden)

    Loyal A Goff

    Full Text Available BACKGROUND: MicroRNAs are required for maintenance of pluripotency as well as differentiation, but since more microRNAs have been computationally predicted in genome than have been found, there are likely to be undiscovered microRNAs expressed early in stem cell differentiation. METHODOLOGY/PRINCIPAL FINDINGS: SOLiD ultra-deep sequencing identified >10(7 unique small RNAs from human embryonic stem cells (hESC and neural-restricted precursors that were fit to a model of microRNA biogenesis to computationally predict 818 new microRNA genes. These predicted genomic loci are associated with chromatin patterns of modified histones that are predictive of regulated gene expression. 146 of the predicted microRNAs were enriched in Ago2-containing complexes along with 609 known microRNAs, demonstrating association with a functional RISC complex. This Ago2 IP-selected subset was consistently expressed in four independent hESC lines and exhibited complex patterns of regulation over development similar to previously-known microRNAs, including pluripotency-specific expression in both hESC and iPS cells. More than 30% of the Ago2 IP-enriched predicted microRNAs are new members of existing families since they share seed sequences with known microRNAs. CONCLUSIONS/SIGNIFICANCE: Extending the classic definition of microRNAs, this large number of new microRNA genes, the majority of which are less conserved than their canonical counterparts, likely represent evolutionarily recent regulators of early differentiation. The enrichment in Ago2 containing complexes, the presence of chromatin marks indicative of regulated gene expression, and differential expression over development all support the identification of 146 new microRNAs active during early hESC differentiation.

  3. Fish the ChIPs: a pipeline for automated genomic annotation of ChIP-Seq data

    Directory of Open Access Journals (Sweden)

    Minucci Saverio

    2011-10-01

    Full Text Available Abstract Background High-throughput sequencing is generating massive amounts of data at a pace that largely exceeds the throughput of data analysis routines. Here we introduce Fish the ChIPs (FC, a computational pipeline aimed at a broad public of users and designed to perform complete ChIP-Seq data analysis of an unlimited number of samples, thus increasing throughput, reproducibility and saving time. Results Starting from short read sequences, FC performs the following steps: 1 quality controls, 2 alignment to a reference genome, 3 peak calling, 4 genomic annotation, 5 generation of raw signal tracks for visualization on the UCSC and IGV genome browsers. FC exploits some of the fastest and most effective tools today available. Installation on a Mac platform requires very basic computational skills while configuration and usage are supported by a user-friendly graphic user interface. Alternatively, FC can be compiled from the source code on any Unix machine and then run with the possibility of customizing each single parameter through a simple configuration text file that can be generated using a dedicated user-friendly web-form. Considering the execution time, FC can be run on a desktop machine, even though the use of a computer cluster is recommended for analyses of large batches of data. FC is perfectly suited to work with data coming from Illumina Solexa Genome Analyzers or ABI SOLiD and its usage can potentially be extended to any sequencing platform. Conclusions Compared to existing tools, FC has two main advantages that make it suitable for a broad range of users. First of all, it can be installed and run by wet biologists on a Mac machine. Besides it can handle an unlimited number of samples, being convenient for large analyses. In this context, computational biologists can increase reproducibility of their ChIP-Seq data analyses while saving time for downstream analyses. Reviewers This article was reviewed by Gavin Huttley, George

  4. Shotgun metagenomic data reveals signifcant abundance but low diversity of Candidatus Scalindua marine anammox bacteria in the Arabian Sea oxygen minimum zone

    Directory of Open Access Journals (Sweden)

    laura eVillanueva

    2014-02-01

    Full Text Available Anaerobic ammonium oxidizing (anammox bacteria are responsible for a significant portion of the loss of fixed nitrogen from the oceans, making them important players in the global nitrogen cycle. To date, marine anammox bacteria found in both water columns and sediments worldwide belong almost exclusively to Candidatus Scalindua species. Recently the genome assembly of a marine anammox enrichment culture dominated by Candidatus Scalindua profunda became available and can now be used as a template to study metagenome data obtained from various oxygen minimum zones. Here, we sequenced genomic DNA from suspended particulate matter recovered at the upper (170 m deep and center (600 m area of the oxygen minimum zone in the Arabian Sea by SOLiD and Ion Torrent technology. The genome of Candidatus Scalindua profunda served as a template to collect reads. Based on the mapped reads marine anammox Abundance was estimated to be at least 0.4% in the upper and 1.7% in the center area. Single nucleotide variation (SNV analysis was performed to assess diversity of the Candidatus Scalindua populations. Most highly covered were the two diagnostic anammox genes hydrazine synthase (scal_01318c, hzsA and hydrazine dehydrogenase (scal_03295, hdh, while other genes involved in anammox metabolism (narGH, nirS, amtB, focA and ACS had a lower coverage but could still be assembled and analyzed. The results show that Candidatus Scalindua is abundantly present in the Arabian Sea OMZ, but that the diversity within the ecosystem is relatively low.

  5. Magnetic Flux Rope Shredding by Quasi-Separatrix Layers: The Detrimental Effects of Magnetic Topology on Solar Eruptions

    Science.gov (United States)

    Chintzoglou, Georgios; Stenborg, Guillermo; Savcheva, Antonia; Vourlidas, Angelos; Tassev, Svetlin; Tun Beltran, Samuel

    2016-07-01

    We present the analysis of an unusual failed eruption event observed in high cadence and in many wavelengths during the campaign in support of the VAULT2.0 sounding rocket launch. The refurbished Very high Angular resolution Ultraviolet Telescope (VAULT2.0) is a Lyalpha (1216AA) spectroheliograph launched on September 30, 2014. The objective of the VAULT2.0 project is the study of the chromosphere-corona interface. The observing campaign targeted active region AR 12172 and was closely coordinated with the textsl{Hinode/} and textsl{IRIS/} missions and several ground-based observatories (NSO/IBIS an SOLIS, and BBSO) ). A filament eruption accompanied by small level heating (at the GOES C-class level) occurred around the VAULT2.0 launch. No CME was observed. The eruption and its source region, however, was recorded by the campaign instruments in all atmospheric heights ranging from the photosphere to the corona in high cadence and spatial resolution. This is a rare occasion which enables us to perform a comprehensive investigation on a failed eruption. We find that a rising Magnetic Flux Rope-like (MFR) structure was destroyed during its interaction with the overlying magnetic field creating downflows of cool plasma and diffuse hot coronal structures reminiscent of 'spines'. We employ MHD simulations to show that the magnetic topology of the overlying field is responsible for the destruction of the MFR. Our unique observations suggest that the magnetic topology of the corona is a key ingredient for a successful eruption.

  6. De novo assembly and characterization of the carrot transcriptome reveals novel genes, new markers, and genetic diversity

    Directory of Open Access Journals (Sweden)

    Matvienko Marta

    2011-08-01

    Full Text Available Abstract Background Among next generation sequence technologies, platforms such as Illumina and SOLiD produce short reads but with higher coverage and lower cost per sequenced nucleotide than 454 or Sanger. A challenge now is to develop efficient strategies to use short-read length platforms for de novo assembly and marker development. The scope of this study was to develop a de novo assembly of carrot ESTs from multiple genotypes using the Illumina platform, and to identify polymorphisms. Results A de novo assembly of transcriptome sequence from four genetic backgrounds produced 58,751 contigs and singletons. Over 50% of these assembled sequences were annotated allowing detection of transposable elements and new carrot anthocyanin genes. Presence of multiple genetic backgrounds in our assembly allowed the identification of 114 computationally polymorphic SSRs, and 20,058 SNPs at a depth of coverage of 20× or more. Polymorphisms were predominantly between inbred lines except for the cultivated x wild RIL pool which had high intra-sample polymorphism. About 90% and 88% of tested SSR and SNP primers amplified a product, of which 70% and 46%, respectively, were of the expected size. Out of verified SSR and SNP markers 84% and 82% were polymorphic. About 25% of SNPs genotyped were polymorphic in two diverse mapping populations. Conclusions This study confirmed the potential of short read platforms for de novo EST assembly and identification of genetic polymorphisms in carrot. In addition we produced the first large-scale transcriptome of carrot, a species lacking genomic resources.

  7. RNA sequencing of the exercise transcriptome in equine athletes.

    Directory of Open Access Journals (Sweden)

    Stefano Capomaccio

    Full Text Available The horse is an optimal model organism for studying the genomic response to exercise-induced stress, due to its natural aptitude for athletic performance and the relative homogeneity of its genetic and environmental backgrounds. Here, we applied RNA-sequencing analysis through the use of SOLiD technology in an experimental framework centered on exercise-induced stress during endurance races in equine athletes. We monitored the transcriptional landscape by comparing gene expression levels between animals at rest and after competition. Overall, we observed a shift from coding to non-coding regions, suggesting that the stress response involves the differential expression of not annotated regions. Notably, we observed significant post-race increases of reads that correspond to repeats, especially the intergenic and intronic L1 and L2 transposable elements. We also observed increased expression of the antisense strands compared to the sense strands in intronic and regulatory regions (1 kb up- and downstream of the genes, suggesting that antisense transcription could be one of the main mechanisms for transposon regulation in the horse under stress conditions. We identified a large number of transcripts corresponding to intergenic and intronic regions putatively associated with new transcriptional elements. Gene expression and pathway analysis allowed us to identify several biological processes and molecular functions that may be involved with exercise-induced stress. Ontology clustering reflected mechanisms that are already known to be stress activated (e.g., chemokine-type cytokines, Toll-like receptors, and kinases, as well as "nucleic acid binding" and "signal transduction activity" functions. There was also a general and transient decrease in the global rates of protein synthesis, which would be expected after strenuous global stress. In sum, our network analysis points toward the involvement of specific gene clusters in equine exercise

  8. RNA sequencing of the exercise transcriptome in equine athletes.

    Science.gov (United States)

    Capomaccio, Stefano; Vitulo, Nicola; Verini-Supplizi, Andrea; Barcaccia, Gianni; Albiero, Alessandro; D'Angelo, Michela; Campagna, Davide; Valle, Giorgio; Felicetti, Michela; Silvestrelli, Maurizio; Cappelli, Katia

    2013-01-01

    The horse is an optimal model organism for studying the genomic response to exercise-induced stress, due to its natural aptitude for athletic performance and the relative homogeneity of its genetic and environmental backgrounds. Here, we applied RNA-sequencing analysis through the use of SOLiD technology in an experimental framework centered on exercise-induced stress during endurance races in equine athletes. We monitored the transcriptional landscape by comparing gene expression levels between animals at rest and after competition. Overall, we observed a shift from coding to non-coding regions, suggesting that the stress response involves the differential expression of not annotated regions. Notably, we observed significant post-race increases of reads that correspond to repeats, especially the intergenic and intronic L1 and L2 transposable elements. We also observed increased expression of the antisense strands compared to the sense strands in intronic and regulatory regions (1 kb up- and downstream) of the genes, suggesting that antisense transcription could be one of the main mechanisms for transposon regulation in the horse under stress conditions. We identified a large number of transcripts corresponding to intergenic and intronic regions putatively associated with new transcriptional elements. Gene expression and pathway analysis allowed us to identify several biological processes and molecular functions that may be involved with exercise-induced stress. Ontology clustering reflected mechanisms that are already known to be stress activated (e.g., chemokine-type cytokines, Toll-like receptors, and kinases), as well as "nucleic acid binding" and "signal transduction activity" functions. There was also a general and transient decrease in the global rates of protein synthesis, which would be expected after strenuous global stress. In sum, our network analysis points toward the involvement of specific gene clusters in equine exercise-induced stress, including

  9. The VAULT2.0 Observing Campaign: A Comprehensive Investigation of the Chromosphere-Corona Interface at Sub-arcsecond scales

    Science.gov (United States)

    Vourlidas, A.; Korendyke, C.; Tun-Beltran, S. D.; Ugarte-Urra, I.; Morrill, J. S.; Warren, H. P.; Young, P.; De Pontieu, B.; Gauzzi, G.; Reardon, K.

    2014-12-01

    We report the first results from an observing campaign in support of the VAULT2.0 sounding rocket launch on September 30, 2014. VAULT2.0 is a Lya (1216Å) spectroheliograph capable of 0.3" (~250 km) spatial resolution. The objective of the VAULT2.0 project is the study of the chromosphere-corona interface. This interface has acquired renewed emphasis over the last few years, thanks to high-resolution observations from Hinode/SOT and EIS instruments and the Lya imaging from the two VAULT flights. The observations have shown that the upper chromosphere may play a more important role in heating the corona and in affecting EUV observations that previously thought: (1) by supplying the mass via Type-II spicules and, (2) by absorbing coronal emission. Many of the required clues for further progress are located in sub-arcsecond structures with temperatures between 10000 and 50000 K, a regime not accessible by Hinode or SDO. Lyman-alpha observations are, therefore, ideal, for filling in this gap. The observing campaign in support of the VAULT2.0 is closely coordinated with the Hinode and IRIS missions to study the mass/energy flow from the chromosphere to the corona with joint observations of type-II spicules, and the magnetic connectivity of coronal loops using the full imaging and spectral capabilities of IRIS, Hinode and SDO. Several ground-based observatories also provide important observations (IBIS, BBSO, SOLIS). The VAULT2.0 project is funded by the NASA LCAS program.

  10. Mucilaginibacter carri sp. nov., isolated from a car air conditioning system.

    Science.gov (United States)

    Kim, Dong-Uk; Lee, Hyosun; Kim, Hyun; Kim, Song-Gun; Park, So Yoon; Ka, Jong-Ok

    2016-04-01

    A Gram-stain-negative, pink bacterial strain, designated PR0008KT, was isolated from an automobile evaporator core in Korea. The cells were obligately aerobic and rod-shaped. The strain grew at 10-40 °C (optimum, 20 °C), at pH 5-8 (optimum, 7), and in the presence of 0-1.5% (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus Mucilaginibacter (93.4-97.0% 16S rRNA sequence similarities) and showed a high sequence similarity with Mucilaginibacter litoreus BR-18T, Mucilaginibacter lutimaris BR-3T and Mucilaginibacter soli R9-65T (97.0%, 96.9% and 96.9% 16S rRNA sequence similarity, respectively). It contained summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16 : 0, iso-C17:0 3-OH and C16:0 as the predominant fatty acids and MK-7 as the major menaquinone. The polar lipids were phosphatidylethanolamine, one unknown aminophospholipid, two unknown aminolipids and two unknown polar lipids. The DNA G+C content of this strain was 47.4 mol%. Based on the phenotypic, genotypic and chemotaxonomic data, strain PR0008KT represents a novel species in the genus Mucilaginibacter, for which the name Mucilaginibacter carri sp. nov. (=KACC 17938T=NBRC 111539T) is proposed. PMID:26827708

  11. Solar magnetic activity cycles, coronal potential field models and eruption rates

    Science.gov (United States)

    Petrie, Gordon

    2013-07-01

    We study the evolution of the observed photospheric magnetic field and the modeled global coronal magnetic field during the past 3 1/2 solar activity cycles observed since the mid-1970s. We use synoptic magnetograms and extrapolated potential-field models based on longitudinal full-disk photospheric magnetograms from the NSO's three magnetographs at Kitt Peak, the Synoptic Optical Long-term Investigations of the Sun (SOLIS) vector spectro-magnetograph (VSM), the spectro-magnetograph and the 512-channel magnetograph instruments, and from the U. Stanford's Wilcox Solar Observatory. The associated multipole field components are used to study the dominant length scales and symmetries of the coronal field. Of the axisymmetric multipoles, only the dipole and octupole follow the poles whereas the higher orders follow the activity cycle. All non-axisymmetric multipole strengths are well correlated with the activity cycle. The axial dipole and octupole are the largest contributors to the global field except while the polar fields are reversing. This influence of the polar fields extends to modulating eruption rates. According to the Computer Aided CME Tracking (CACTus), Solar Eruptive Event Detection System (SEEDS), and Nobeyama radioheliograph prominence eruption catalogs, the rate of solar eruptions is found to be systematically higher for active years between 2003-2012 than for those between 1997-2002. This behavior appears to be connected with the weakness of the late-cycle 23 polar fields as suggested by Luhmann. We see evidence that the process of cycle 24 field reversal is well advanced at both poles.

  12. INVESTIGATING THE CORRELATION BETWEEN ACADEMIC AVARAGES OF ELEMENTARY ENGLISH COURSE AND ENGLISH SUB-TEST SCORES IN LEVEL DETERMINATION EXAMINATION WITH STRUCTURAL EQUATION MODELLING

    Directory of Open Access Journals (Sweden)

    Gökhan BAŞ

    2013-06-01

    Full Text Available Purpose and Significance: The central examinations have been per¬formed with the aim of selecting and placing the students in the various education institutions for a long time in Turkey. Among these central examinations come the ones that are performed in the transition from elementary education to secondary education first (Karakaya, 2012. In the transition from elementary education to sec¬ondary education, the results of the Level Determination Examination, known as SBS are used. In order to select students and place them in second¬ary schools, the Level Determination Examination (LDE has been performed for the 6th, 7th, and 8th grades since the academic year 2007–2008 (MEB, 2010a. The Level Determination Examination includes five sub-tests, which are Turkish, mathematics, science and technology, social sciences, and foreign languages (MEB, 2010b. After the elementary curriculum has been renewed in 2005-2006 academic year, alternative measurement and assessment approaches are being used in the classrooms such as project work, classroom involvement, performance work, portfolio, etc. (Atılgan, Kan & Doğan, 2011. Amongst these, project work, classroom involvement, performance work, and formal examinations at school are being used for the transition from elementary education to sec¬ondary education as well as the result of the Level Determination Examination (MEB, 2010a. When the related literature is viewed, it is seen that there are many studies on the effects of the LDE (Sevindik, 2009; Üzkurt & Koçakoğlu, 2009; Anıl et al., 2010; Güler, 2010; Erdoğan, Giorgietti & Çifçili, 2010; Kelecioğlu, Atalay & Öztürk, 2010; Kurt, 2010; Yılmaz, 2010; Anıl & Güzeller, 2011; Bal, 2011; Şahin, 2011; Güzeller, 2012; Karakaya, 2012; Karakaya & Kutlu, 2012. These studies are seen that they have mostly focused on mathematics and science and technology sub-tests in the LDE. However, it is understood that there are no studies which focuses

  13. Commercial-Scale Demonstration of the Liquid Phase Methanol (LPMEOH) Process

    Energy Technology Data Exchange (ETDEWEB)

    None

    1998-12-21

    The Liquid Phase Methanol (LPMEOW) Demonstration Project at Kingsport Tennessee, is a $213.7 million cooperative agreement between the U.S. Department of Energy (DOE) and Air Products Liquid Phase Conversion Company, L.P. (the Partnership) to produce methanol from coal-derived synthesis gas (syngas). Air Products and Chemicals, Inc. (Air Products) and Eastman Chemical Company (Eastman) formed the Partnership to execute the Demonstration Project. The LPMEOW Process Demonstration Unit was built at a site located at the Eastman complex in Kingsport. During this quarter, initial planning and procurement work began on the seven project sites which have been accepted for participation in the off-site, methanol product-use test plan. Two of the projects have begun pre-testing of equipment and three other projects have commenced with equipment procurement, Methanol produced from carbon monoxide (CO)- rich syngas at the Alternative Fuels Development Unit (AFDU) in LaPorte, TX has been shipped to four of the project sites in anticipation of the start of testing during the first quarter of calendar year 1998. Catalyst activity, as defined by the ratio of the rate constant at any point in time to the rate constant for a freshly reduced catalyst (as determined in the laboratory autoclave), continued to decline more rapidly than expected. In response to concentrations of arsenic and sulfbr detected on catalyst samples from the LPMEOW Reactor, Eastman replaced both the arsine- and sulfiwremoval material in the Eastman guard bed which treats the primary syngas feed stream (&danced Gas) prior to its introduction into both the Eastman fixed-bed methanol plant and the LPMEOWM Demonstration Unit. After restarting the demonstration unit, the catalyst deactivation rate remained essentially unchanged. Parallel testing in the laboratory using arsine-doped, and subsequently arsine- and SuIfi-doped syngas, ako ftiIed to prove that arsine was responsible for the higher-than-expected rate of

  14. Neki problemi pri prevođenju moderne poezije promatrani kroz teoriju konceptualne integracije

    Directory of Open Access Journals (Sweden)

    Sanja Cimer

    2011-12-01

    koji slijede u vlastitom su prijevodu, osim ako nije drugačije naznačeno. U 20. stoljeću oformio se, između ostalih, pristup da je zadatak prevoditelja prijevodom oponašati izvorno lirsko djelo, a uz to što prijevod treba preslikavati original, on također treba zadržati i njegovu poetsku vrijednost te u ciljnom jeziku funkcionirati kao samostalna poetska forma (bez glosa, komentara ili fusnota. Budući da je svaka pjesma priča za sebe, svaki je prevoditelj pri definiranju prioriteta (zvučnost? semantika? rima? ritam? prepušten samom sebi i konstantno se suočava s izborima (Jones (citat u Connolly 174 prevođenje poezije naziva “umjetnošću kompromisa”. W. S. Merwin opisuje svoj pristup prevođenju poezije na sljedeći način: “Znate, i dalje vjerujem da ne znam kako se prevodi i da to nitko ne zna. To je nemoguć, ali nužan proces, ne postoji savršen način da se on izvede i velik njegov dio mora se oblikovati u hodu za svaku pojedinačnu pjesmu.”

  15. Quo vadis hrvatsko športsko pravo (posebno na primjeru nacionalnog športskog saveza u stečaju i pretvorbe športskih objekata

    Directory of Open Access Journals (Sweden)

    Hrvoje Kačer

    2011-12-01

    Full Text Available Športsko pravo je dosta mlada pravna grana, što po naravi stvari znači i opasnost od određenih nedostataka kojih nema u tradicionalnim pravnim granama kao što je npr. građansko ili kazneno pravo. Obzirom na pravno načelo koje kao trajno vrijedi u pravu općenito lex specialis derogat legi generali relativno je lako kada ta posebna (športska u ovom slučaju pravna norma postoji. Međutim, kada se u jednom tako naglašeno posebnom području (prepunom specifičnosti kao što je šport dogodi da posebne pravne norme nema i kada je nužno primijeniti onu pravnu normu kod čijeg donositelja zasigurno u razmišljanju nije bilo športa, nastaje problem, ponekad čak i prava pravna katastrofa. Drukčije sigurno nije moguće ocijeniti situaciju postojanja normative koja spriječava osnivanje drugog nacionalnog saveza dok postoji onaj nad kojim je otvoren stečaj, a još više kada dođe do otkupa tražbina prema stečajnom dužniku i na taj način preuzimanja stečajnog dužnika sa svim pravima (uključujući i onaj članstva u Hrvatskom olimpijskom odboru kao da se radi o stečaju neke tvornice npr. Jedini izlaz je u donošenju posebne, izvorno športske specijalističke pravne norme, ako je ikako moguće čak i uz tzv. povratni ili retroaktivni učinak te norme. Neposredan povod ovom tekstu su događanja u hrvatskom športu na način da su dva hrvatska nacionalna športska saveza (odbojka i golf doživjela stečajni postupak i primjenu onih pravnih normi (jer drugih nema koja vrijede npr. za stečaj trgovačkih društava.Nažalost, reakcija hrvatskog zakonodavca na način koji je bio za očekivati (ali i zahtijevati je izostala i otvorila (još jednom ustavnopravno pitanje da li i što učiniti (unutar sustava trodiobe vlasti i kako sankcionirati (osim rezultatom na izborima nedopustivu pasivnost zakonodavca koja se na primjeru športskog prava dogodila već više puta, a kao stalno obilježje u pravu općenito je postojeća i dogodila se i doga

  16. Crecimiento y supervivencia de crías de pulpo (Octopus maya : alimentados con dietas inertes bajo condiciones de laboratorio en Yucatán, México Growth and survival of larvals of octopus (Octopus maya : fed with inert diets under conditions of laboratory in Yucatan, Mexico

    Directory of Open Access Journals (Sweden)

    Miguel Rodríguez-Serna

    2008-12-01

    Full Text Available Bajo un diseño experimental se probaron tres dietas con el objetivo de determinar el crecimiento y la supervivencia de las crías de Octopus maya (Voss y Solís 1966, utilizando dos tipos de alimento inerte (A2 = Balanceado, A3 = Surimi y comparándolo con un alimento vivo (A1 = Artemia, bajo condiciones controladas. Los resultados mostraron diferencias significativas entre el alimento vivo y el alimento inerte. Se encontró un mejor crecimiento en peso ganado en las crías de pulpo alimentadas con una dieta viva en comparación con las dietas inertes (A1 = 11.88 g, A2 = 8.9 g, A3 = 8.83 g; sin embargo, para las tres dietas se obtuvo una baja supervivencia. El análisis estadístico arrojó diferencias estadísticas (p > 0.05 entre todos los tratamientos, siendo el alimento vivo el que presentó la mayor supervivencia con un 61.3%, seguido del surimi con un 42.3% y finalmente el balanceado con un 27.6%. La alimentación de O. maya en cautiverio aún requiere de muchos esfuerzos para desarrollar un alimento balanceado adecuado desde el punto de vista nutrimental o de formulación, así como desde la parte de atracción y aceptación por los animales.Three experimental diets were probed for the growth and survival rates of young Octopus maya (Voss and Solis 1966, using two inert foods (A2 = shrimp pellets, A3 = Surimi and one alive food (A1 = Artemia, under controlled conditions. The results showed significant differences between the live food and the inert ones. Young octopus feed with artemia, reported better growth in weight gained compared with the inert diets (A1 = 11.88 g, A2 = 8.9 g, A3 = 8.83 g. All three diets obtained low survivorships, being the alive food the best one with a rate of 61.3%, followed by surimi with 42.3%, and finally the pellet food with 27.6%. Statistical analysis showed differences (p > 0.05 between all the treatments for both growth and survival results. Artificial feeding of O. maya in captivity still requires many

  17. Students' Sex Mentality of One Medical College%某医学院本科生性心理现状分析

    Institute of Scientific and Technical Information of China (English)

    李志刚; 马长征

    2009-01-01

    目的 了解医学生性心理现状,为建立高校性心理健康教育模式提供依据.方法 采用自编问卷对某医学院399名本科医学生进行问卷调查.结果 男、女生是否喜欢自身性别的报告率差异有统计学意义(P<0.01),男生更愿意接受自身性别;大多数学生都希望与异性交往,且男生的需求更强烈;男、女生在释放性心理压力的途径与方法上差异有统计学意义(P<0.05);多数学生认为大学期间恋爱动机是排遣寂寞.结论 医学生性发展与性知识不协调,性需求和性满足之间存在冲突和矛盾.应科学引导学生树立正确的恋爱观,重视在校园内开展性知识教育和性文化建设.%Objective To understand medical students" current sex mentality, and to provide bases for establishing sex mental health edu-cation mode. Methods By using self-designed questionnaire, 399 medical students were investigated. Results There was statistical significant difference about the opinion towards whether they like their genders or not(P <0.01) , male students were more willing to accept their sex identities. Moat students were expecting to sooialize to socialize with the opposite genders and the male students manifested stronger desire. There were huge gen-der differences in the ways to releasing sex mental pressure (P < 0.05). Most students regarded love affaires in campus as a way of casting off soli-tude. Conclusion Medical college students" sex development and sex knowledge are not coordinated. There are conflicts between sex desire and fulfillment. It's advisable to help students develop proper ideas about love by sex education and spreading of its related culture.

  18. Proposal of nine novel species of the genus Lysinimicrobium and emended description of the genus Lysinimicrobium.

    Science.gov (United States)

    Hamada, Moriyuki; Shibata, Chiyo; Saitou, Satomi; Tamura, Tomohiko; Komaki, Hisayuki; Ichikawa, Natsuko; Oguchi, Akio; Hosoyama, Akira; Fujita, Nobuyuki; Yamamura, Hideki; Hayakawa, Masayuki; Suzuki, Ken-Ichiro

    2015-12-01

    Thirteen novel Gram-stain-positive bacteria were isolated from various samples collected from mangrove forests in Japan, and their taxonomic positions were investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequence comparisons showed that the 13 isolates formed a single clade with Lysinimicrobium mangrovi HI08-69T, with a similarity range of 97.6-99.5 %. The peptidoglycan of the isolates was of the A4α type with an interpeptide bridge comprising Ser-Glu and an l-Ser residue at position 1 of the peptide subunit. The predominant menaquinone was demethylmenaquinone DMK-9(H4) and the major fatty acid was anteiso-C15 : 0. These chemotaxonomic characteristics corresponded to those of the genus Lysinimicrobium. On the basis of the phenotypic and phylogenetic data, along with average nucleotide identity values among the isolates, we concluded that the 13 isolates should be assigned to the following nine novel species of the genus Lysinimicrobium: Lysinimicrobium aestuarii sp. nov. (type strain HI12-104T = NBRC 109392T = DSM 28144T), Lysinimicrobium flavum sp. nov. (type strain HI12-45T = NBRC 109391T = DSM 28150T), Lysinimicrobium gelatinilyticum sp. nov. (type strain HI12-44T = NBRC 109390T = DSM 28149T), Lysinimicrobium iriomotense sp. nov. (type strain HI12-143T = NBRC 109399T = DSM 28146T), Lysinimicrobium luteum sp. nov. (type strain HI12-123T = NBRC 109395T = DSM 28147T), Lysinimicrobium pelophilum sp. nov. (type strain HI12-111T = NBRC 109393T = DSM 28148T), Lysinimicrobium rhizosphaerae sp. nov. (type strain HI12-135T = NBRC 109397T = DSM 28152T), Lysinimicrobium soli sp. nov. (type strain HI12-122T = NBRC 109394T = DSM 28151T) and Lysinimicrobium subtropicum sp. nov. (type strain HI12-128T = NBRC 109396T = DSM 28145T). In addition, an emended description of the genus Lysinimicrobium is proposed.

  19. An evaluation of alternative methods for constructing phylogenies from whole genome sequence data: a case study with Salmonella

    Directory of Open Access Journals (Sweden)

    James B. Pettengill

    2014-10-01

    Full Text Available Comparative genomics based on whole genome sequencing (WGS is increasingly being applied to investigate questions within evolutionary and molecular biology, as well as questions concerning public health (e.g., pathogen outbreaks. Given the impact that conclusions derived from such analyses may have, we have evaluated the robustness of clustering individuals based on WGS data to three key factors: (1 next-generation sequencing (NGS platform (HiSeq, MiSeq, IonTorrent, 454, and SOLiD, (2 algorithms used to construct a SNP (single nucleotide polymorphism matrix (reference-based and reference-free, and (3 phylogenetic inference method (FastTreeMP, GARLI, and RAxML. We carried out these analyses on 194 whole genome sequences representing 107 unique Salmonella enterica subsp. enterica ser. Montevideo strains. Reference-based approaches for identifying SNPs produced trees that were significantly more similar to one another than those produced under the reference-free approach. Topologies inferred using a core matrix (i.e., no missing data were significantly more discordant than those inferred using a non-core matrix that allows for some missing data. However, allowing for too much missing data likely results in a high false discovery rate of SNPs. When analyzing the same SNP matrix, we observed that the more thorough inference methods implemented in GARLI and RAxML produced more similar topologies than FastTreeMP. Our results also confirm that reproducibility varies among NGS platforms where the MiSeq had the lowest number of pairwise differences among replicate runs. Our investigation into the robustness of clustering patterns illustrates the importance of carefully considering how data from different platforms are combined and analyzed. We found clear differences in the topologies inferred, and certain methods performed significantly better than others for discriminating between the highly clonal organisms investigated here. The methods supported by

  20. Fast Solar Polarimeter: Prototype Characterization and First Results

    Science.gov (United States)

    Iglesias, F. A.; Feller, A.; Krishnappa, N.; Solanki, S. K.

    2016-04-01

    Due to the differential and non-simultaneous nature of polarization measurements, seeing induced crosstalk (SIC) and seeing limited spatial resolution can easily counterbalance the benefits of solar imaging polarimetry from the ground. The development of instrumental techniques to treat these issues is necessary to fully exploit the next generation of large-aperture solar facilities, and maintain ground-based data at a competitive level with respect to its space-based counterpart. In particular, considering that many open questions in modern solar physics demand data with challenging specifications of resolution and polarimetric sensitivity that can only be achieved with large telescope apertures (Stenflo 1999). Even if state-of-the-art adaptive optics systems greatly improve image quality, their limited correction —due to finite bandwidth, mode number and seeing anisoplanat- ism— produces large residual values of SIC (Krishnappa & Feller 2012). Dual beam polarimeters are commonly used to reduce SIC between the intensity and polarization signals, however, they cannot compensate for the SIC introduced between circular and linear polarization, which can be relevant for high-precision polarimetry. It is known that fast modulation effectively reduces SIC, but the demodulation of the corresponding intensity signals imposes hard requirements on the frame rate of the associated cameras. One way to avoid a fast sensor, is to decouple the camera readout from the intensity demodulation step. This concept is the cornerstone of the very successful Zurich Imaging Polarimeter (ZIMPOL). Even though the ZIMPOL solution allows the detection of very faint signals (˜10-5), its design is not suitable for high-spatial-resolution applications. We are developing a polarimeter that focuses on both spatial resolution (package is similar to the SOLIS (Keller et al. 2003) design and optimized to have an achromatic total polarimetric efficiency above 80 % in the 400-700 nm wavelength

  1. Oomycete transcriptomics database: A resource for oomycete transcriptomes

    Directory of Open Access Journals (Sweden)

    Tripathy Sucheta

    2012-07-01

    Full Text Available Abstract Background Oomycete pathogens have attracted significant attention in recent years due to their economic impact. With improving sequencing technologies, large amounts of oomycete transcriptomics data are now available which have great biological utility. A known bottleneck with next generation sequencing data however lies with their analysis, interpretation, organization, storage and visualization. A number of efforts have been made in this respect resulting in development of a myriad of resources. Most of the existing NGS browsers work as standalone applications that need processed data to be uploaded to the browser locally for visualization. At the same time, several oomycete EST databases such as PFGD, ESTAP and SPC, are not available anymore, so there is an immediate need for a database resource that can store and disseminate this legacy information in addition to NGS data. Description Oomycetes Transcriptomics Database is an integrated transcriptome and EST data resource for oomycete pathogens. The database currently stores processed ABI SOLiD transcript sequences from Phytophthora sojae and its host soybean (P. sojae mycelia, healthy soybean and P. sojae-infected soybean as well as Illumina transcript sequences from five Hyaloperonospora arabidopsidis libraries. In addition to those resources, it has also a complete set of Sanger EST sequences from P. sojae, P. infestans and H. arabidopsidis grown under various conditions. A web-based transcriptome browser was created for visualization of assembled transcripts, their mapping to the reference genome, expression profiling and depth of read coverage for particular locations on the genome. The transcriptome browser merges EST-derived contigs with NGS-derived assembled transcripts on the fly and displays the consensus. OTD possesses strong query features and the database interacts with the VBI Microbial Database as well as the Phytophthora Transcriptomics Database. Conclusion Oomycete

  2. Identification of microRNA genes in three opisthorchiids.

    Directory of Open Access Journals (Sweden)

    Vladimir Y Ovchinnikov

    2015-04-01

    Full Text Available Opisthorchis felineus, O. viverrini, and Clonorchis sinensis (family Opisthorchiidae are parasitic flatworms that pose a serious threat to humans in some countries and cause opisthorchiasis/clonorchiasis. Chronic disease may lead to a risk of carcinogenesis in the biliary ducts. MicroRNAs (miRNAs are small noncoding RNAs that control gene expression at post-transcriptional level and are implicated in the regulation of various cellular processes during the parasite- host interplay. However, to date, the miRNAs of opisthorchiid flukes, in particular those essential for maintaining their complex biology and parasitic mode of existence, have not been satisfactorily described.Using a SOLiD deep sequencing-bioinformatic approach, we identified 43 novel and 18 conserved miRNAs for O. felineus (miracidia, metacercariae and adult worms, 20 novel and 16 conserved miRNAs for O. viverrini (adult worms, and 33 novel and 18 conserved miRNAs for C. sinensis (adult worms. The analysis of the data revealed differences in the expression level of conserved miRNAs among the three species and among three the developmental stages of O. felineus. Analysis of miRNA genes revealed two gene clusters, one cluster-like region and one intronic miRNA in the genome. The presence and structure of the two gene clusters were validated using a PCR-based approach in the three flukes.This study represents a comprehensive description of miRNAs in three members of the family Opistorchiidae, significantly expands our knowledge of miRNAs in multicellular parasites and provides a basis for understanding the structural and functional evolution of miRNAs in these metazoan parasites. Results of this study also provides novel resources for deeper understanding the complex parasite biology, for further research on the pathogenesis and molecular events of disease induced by the liver flukes. The present data may also facilitate the development of novel approaches for the prevention and

  3. Sphingomonas aerophila sp. nov. and Sphingomonas naasensis sp. nov., isolated from air and soil, respectively.

    Science.gov (United States)

    Kim, Soo-Jin; Moon, Ji-Young; Lim, Jun-Muk; Ahn, Jae-Hyung; Weon, Hang-Yeon; Ahn, Tae-Young; Kwon, Soon-Wo

    2014-03-01

    Two strains, designated 5413J-26(T) and KIS18-15(T), were isolated from the air and forest soil, respectively, in South Korea. Cells of the two strains were Gram-stain-negative, aerobic, polar-flagellated and rod-shaped. According to the phylogenetic tree, strains 5413J-26(T) and KIS18-15(T) fell into the cluster of Sphingomonas sensu stricto. Strain 5413J-26(T) showed the highest sequence similarities with Sphingomonas trueperi LMG 2142(T) (96.6%), Sphingomonas molluscorum KMM 3882(T) (96.5%), Sphingomonas azotifigens NBRC 15497(T) (96.3 %) and Sphingomonas pituitosa EDIV(T) (96.1 %), while strain KIS18-15(T) had the highest sequence similarity with Sphingomonas soli T5-04(T) (96.8%), Sphingomonas pituitosa EDIV(T) (96.6%), Sphingomonas leidyi ATCC 15260(T) (96.6 %), Sphingomonas asaccharolytica NBRC 15499(T) (96.6 %) and Sphingomonas koreensis JSS26(T) (96.6 %). The 16S rRNA gene sequence similarity between strains 5413J-26(T) and KIS18-15(T) was 95.4 %. Ubiquinone 10 was the predominant respiratory quinone and homospermidine was the major polyamine. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and several unidentified phospholipids and lipids. The main cellular fatty acids (>10% of the total fatty acids) of strain 5413J-26(T) were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C14 : 0 2-OH, and those of strain KIS18-15(T) were summed feature 8 and C16 : 0. Based on the results of 16S rRNA gene sequence analysis, and physiological and biochemical characterization, two novel species with the suggested names Sphingomonas aerophila sp. nov. (type strain 5413J-26(T) = KACC 16533(T) = NBRC 108942(T)) and Sphingomonas naasensis sp. nov. (type strain KIS18-15(T) = KACC 16534(T) = NBRC 108943(T)) are proposed. PMID:24425812

  4. Educare educando all'opera: socialità e individualità nella 'Fanciulla del West' di Puccini

    Directory of Open Access Journals (Sweden)

    Andrea Chegai

    2014-12-01

    Full Text Available In svariate occasioni i musicologi hanno posto l’accento sull’efficacia educativa dell’opera per lo sviluppo della dimensione sentimentale di soggetti a diversi livelli scolari. Insegnare a cogliere la valenza drammatica di una scena o di un’aria e delle passioni che la animano tramite i contenuti del testo verbale e l’assetto musicale stabilito dal compositore è un ottimo viatico per educare all’ascolto della musica cogliendo le opportunità che essa offre a livello di formazione generale. A questo aspetto potrebbe essere utilmente affiancata la componente interrelazionale che costituisce la sostanza di cui si approvvigiona il teatro musicale di ogni epoca e tradizione. In quanto dramma, l’opera in musica inscena vicende fondate su relazioni interpersonali molteplici; sodalizi e dissidi, legami familiari e amorosi, passioni apparenti o tacite, aspirazioni legittime o illecite e ostacoli che si frappongono al loro conseguimento, socialità o viceversa solitudini ed isolamento. A ognuna di queste condizioni è nella finzione drammatica garantito diritto di esistenza. Persino la crudeltà o l’inganno possono mostrarsi seducenti, in un duetto, ma un ascolto competente riesce a darne conto e a smascherarli. La solitudine di un monologo procura all’opera di ogni tempo esiti musicalmente ragguardevoli, e anche in questo caso se ne può trarre un utile in termini di educazione affettiva (non c’è niente di sbagliato nel sentirsi soli e diversi dagli altri: è uno stato che serve alla crescita e può persino generare bellezza. Buoni e cattivi si confrontano, e come nella vita reale capita che tanto gli uni quanto gli altri possano avere la meglio: l’intreccio del dramma aiuterà a comprendere cosa eventualmente è andato storto; e come nella vita reale capita anche che buoni e cattivi si confondano fra loro. L’opera, contraddistinta dalla presentificazione specifica dei generi performativi, e anche per questo di forte presa

  5. Comprehensive Assessments of RNA-seq by the SEQC Consortium: FDA-Led Efforts Advance Precision Medicine.

    Science.gov (United States)

    Xu, Joshua; Gong, Binsheng; Wu, Leihong; Thakkar, Shraddha; Hong, Huixiao; Tong, Weida

    2016-01-01

    Studies on gene expression in response to therapy have led to the discovery of pharmacogenomics biomarkers and advances in precision medicine. Whole transcriptome sequencing (RNA-seq) is an emerging tool for profiling gene expression and has received wide adoption in the biomedical research community. However, its value in regulatory decision making requires rigorous assessment and consensus between various stakeholders, including the research community, regulatory agencies, and industry. The FDA-led SEquencing Quality Control (SEQC) consortium has made considerable progress in this direction, and is the subject of this review. Specifically, three RNA-seq platforms (Illumina HiSeq, Life Technologies SOLiD, and Roche 454) were extensively evaluated at multiple sites to assess cross-site and cross-platform reproducibility. The results demonstrated that relative gene expression measurements were consistently comparable across labs and platforms, but not so for the measurement of absolute expression levels. As part of the quality evaluation several studies were included to evaluate the utility of RNA-seq in clinical settings and safety assessment. The neuroblastoma study profiled tumor samples from 498 pediatric neuroblastoma patients by both microarray and RNA-seq. RNA-seq offers more utilities than microarray in determining the transcriptomic characteristics of cancer. However, RNA-seq and microarray-based models were comparable in clinical endpoint prediction, even when including additional features unique to RNA-seq beyond gene expression. The toxicogenomics study compared microarray and RNA-seq profiles of the liver samples from rats exposed to 27 different chemicals representing multiple toxicity modes of action. Cross-platform concordance was dependent on chemical treatment and transcript abundance. Though both RNA-seq and microarray are suitable for developing gene expression based predictive models with comparable prediction performance, RNA-seq offers

  6. High throughput sequencing in mice: a platform comparison identifies a preponderance of cryptic SNPs

    Directory of Open Access Journals (Sweden)

    Darakjian Priscila

    2009-08-01

    Full Text Available Abstract Background Allelic variation is the cornerstone of genetically determined differences in gene expression, gene product structure, physiology, and behavior. However, allelic variation, particularly cryptic (unknown or not annotated variation, is problematic for follow up analyses. Polymorphisms result in a high incidence of false positive and false negative results in hybridization based analyses and hinder the identification of the true variation underlying genetically determined differences in physiology and behavior. Given the proliferation of mouse genetic models (e.g., knockout models, selectively bred lines, heterogeneous stocks derived from standard inbred strains and wild mice and the wealth of gene expression microarray and phenotypic studies using genetic models, the impact of naturally-occurring polymorphisms on these data is critical. With the advent of next-generation, high-throughput sequencing, we are now in a position to determine to what extent polymorphisms are currently cryptic in such models and their impact on downstream analyses. Results We sequenced the two most commonly used inbred mouse strains, DBA/2J and C57BL/6J, across a region of chromosome 1 (171.6 – 174.6 megabases using two next generation high-throughput sequencing platforms: Applied Biosystems (SOLiD and Illumina (Genome Analyzer. Using the same templates on both platforms, we compared realignments and single nucleotide polymorphism (SNP detection with an 80 fold average read depth across platforms and samples. While public datasets currently annotate 4,527 SNPs between the two strains in this interval, thorough high-throughput sequencing identified a total of 11,824 SNPs in the interval, including 7,663 new SNPs. Furthermore, we confirmed 40 missense SNPs and discovered 36 new missense SNPs. Conclusion Comparisons utilizing even two of the best characterized mouse genetic models, DBA/2J and C57BL/6J, indicate that more than half of naturally

  7. Intoxicaciones agudas en Santiago de Compostela,en un período de cuatro años

    Directory of Open Access Journals (Sweden)

    P. Fernández

    2003-01-01

    Full Text Available El objetivo de este trabajo fue evaluar las caracterís-ticas de un grupo de pacientes intoxicados en el área sanitaria deSantiago de Compostela. Para ello, se estudiaron 1192 intoxica-ciones agudas atendidas en el Servicio de Urgencias y Unidadde Cuidados Intensivos del Hospital Clínico Universitario deesta ciudad, desde el 1 de enero de 1993 al 31 de diciembre de1996. Se han considerado solo aquellos casos en los que se soli-citó un análisis toxicológico a este Instituto de Medicina Legal,desconociéndose el número de casos excluidos. Se diseñó unaficha clínica con todos los parámetros estudiados para su cum-plimentación por los médicos en el momento del ingreso. Seobservó un predominio del sexo masculino (61,4% y una edadmedia, en la población estudiada, de 32,3±12,5 años (rango: 15-93 años. Los tóxicos más frecuentemente implicados fueron lasdrogas de abuso (681 casos, seguidas de los fármacos (545casos y de “otros agentes” (88 casos. Las intoxicaciones vo-luntarias estaban asociadas mayoritariamente con mujeres y coningesta de fármacos, en contra de las accidentales, asociadas conhombres y con consumo de drogas de abuso. Existen anteceden-tes psiquiátricos en 332 casos, tratamiento farmacológico previoen 219 casos, alcoholismo en 189 casos y drogadicción por víaparenteral en 144 casos. Se ha constatado que un 76% de lospacientes presentaban alguna clínica al ingreso, con un predo-minio de las manifestaciones neurológicas (682 casos. Se utili-zó algún tipo de tratamiento específico en 574 sujetos, desta-cando la aplicación de lavado gástrico (444 casos y la adminis-tración de antídotos (358 casos. La mayoría de los pacientesevolucionó satisfactoriamente, con solo un 2% de mortalidad.Esta información permite conocer los patrones de abuso y la efi-cacia del tratamiento aplicado con el fin de mejorar el controldel paciente intoxicado

  8. COME EDUCARE LE LETTRICI/I LETTORI

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    Mirjana N. Benjak

    2010-02-01

    Full Text Available Lo scopo principale dell’educazione letteraria nell’ambito della scuola media è formare la futura lettrice/il futuro lettore. La realizzazione di questo obiettivo è affidata principalmente alla lettura d’obbligo. I risultati dell’analisi comparativa dei programmi d’insegnamento (curriculum dei licei croati ed europei, indicano che esistono diversi approcci riguardo la scelta degli autori e dei titoli delle letture d’obbligo. In alcuni casi (ad esempio, gli sloveni si prescrive rigorosamente il numero degli autori e delle opere per ogni anno scolastico, in altri (ad esempio, gli austriaci si prevede la libera scelta delle letture d’obbligo che le alunne e gli alunni concordano assieme alle loro insegnanti ed ai loro insegnanti (lettura d’obbligo concordata, oppure gli insegnanti consigliano solo alcune opere non indicando i nomi degli autori (ad esempio, i cechi. Nei programmi d’insegnamento dei licei croati non esiste alcuna indicazione riguardante la lettura d’obbligo. I dati dell’analisi dimostrano che nella maggioranza dei casi nell’ideazione dei programmi d’insegnamento (curriculum europei non si presta la dovuta attenzione agli interessi ed ai gusti letterari delle alunne/degli alunni. Anche se in Croazia non è stata mai condotta una ricerca istituzionale su questo argomento, i risultati ottenuti sulla base delle singole ricerche sono tali che dovrebbero essere presi in considerazione nella programmazione delle letture d’obbligo nell’ambito dell’insegnamento della letteratura nei licei. In base a questi dati si auspica l’applicazione del cosiddetto sistema aperto di lettura d’obbligo il quale prevede la proposta di un elenco di opere letterarie dal quale le alunne/gli alunni scelgono da soli i testi per la lettura domestica. Al contempo, si propongono procedimenti e metodi che contribuiscono ad eliminare gli ostacoli che l’alunno/a incontra nella comprensione delle singole opere letterarie

  9. Comprehensive Assessments of RNA-seq by the SEQC Consortium: FDA-Led Efforts Advance Precision Medicine

    Directory of Open Access Journals (Sweden)

    Joshua Xu

    2016-03-01

    Full Text Available Studies on gene expression in response to therapy have led to the discovery of pharmacogenomics biomarkers and advances in precision medicine. Whole transcriptome sequencing (RNA-seq is an emerging tool for profiling gene expression and has received wide adoption in the biomedical research community. However, its value in regulatory decision making requires rigorous assessment and consensus between various stakeholders, including the research community, regulatory agencies, and industry. The FDA-led SEquencing Quality Control (SEQC consortium has made considerable progress in this direction, and is the subject of this review. Specifically, three RNA-seq platforms (Illumina HiSeq, Life Technologies SOLiD, and Roche 454 were extensively evaluated at multiple sites to assess cross-site and cross-platform reproducibility. The results demonstrated that relative gene expression measurements were consistently comparable across labs and platforms, but not so for the measurement of absolute expression levels. As part of the quality evaluation several studies were included to evaluate the utility of RNA-seq in clinical settings and safety assessment. The neuroblastoma study profiled tumor samples from 498 pediatric neuroblastoma patients by both microarray and RNA-seq. RNA-seq offers more utilities than microarray in determining the transcriptomic characteristics of cancer. However, RNA-seq and microarray-based models were comparable in clinical endpoint prediction, even when including additional features unique to RNA-seq beyond gene expression. The toxicogenomics study compared microarray and RNA-seq profiles of the liver samples from rats exposed to 27 different chemicals representing multiple toxicity modes of action. Cross-platform concordance was dependent on chemical treatment and transcript abundance. Though both RNA-seq and microarray are suitable for developing gene expression based predictive models with comparable prediction performance, RNA

  10. Experimental study on soil moisture content of geogrid-reinforced clay under freezing-thawing cycle%冻融循环下格栅加筋粘土土体含水率试验研究

    Institute of Scientific and Technical Information of China (English)

    赵荣飞; 宓永宁; 姜广田; 金丹; 高微

    2012-01-01

    通过一系列室内含水率测试试验,研究了格栅加筋粘土在多次冻融循环条件下土体含水率的变动情况,探讨了填料压实度,加筋层数和初始含水率对冻融后土体含水率变动的影响规律.结果表明,压实度对土体ωi影响最大,低压实度时,△ω1为正值,高压实度时,△ω1为负值;加筋层数次之,ω1值随加筋层数增加而减小,在压实度高的土体中尤为显著;初始含水率影响最弱,仅在土体压实度较低时,较大的初始含水率会引起较大的△ω1值.%Through a series of lab-tests on moisture content of soil, the change of the moisture content of the grid-reinforced clay under freezing-thawing cycle is studied herein, and then the law of the impact from the compaction degree, the reinforcement layers and the initial moisture content of the soil on the moisture content of the soil under freezing-thawing cycles are discussed. The result shows that the compaction degree has the greatest impact on the soil body ωi, i. e. △ω1 is positive when the compaction degree is low and △ω1, is negative when the compaction degree is high; meanwhile, the number of the reinforcement layers only has a secondary impact on the soli body, i. e. the value of ω1, is decreased along with the increase of the number of the reinforcement layers, while it is significant in the highly compacted soil; moreover, the impact from the initial moisture content is much less and the greater value of △ω1 is to be only arisen from a greater initial moisture content when the compaction degree of soil is low.

  11. Transcriptome profiling of the demosponge Amphimedon queenslandica reveals genome-wide events that accompany major life cycle transitions

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    Conaco Cecilia

    2012-05-01

    Full Text Available Abstract Background The biphasic life cycle with pelagic larva and benthic adult stages is widely observed in the animal kingdom, including the Porifera (sponges, which are the earliest branching metazoans. The demosponge, Amphimedon queenslandica, undergoes metamorphosis from a free-swimming larva into a sessile adult that bears no morphological resemblance to other animals. While the genome of A. queenslandica contains an extensive repertoire of genes very similar to that of complex bilaterians, it is as yet unclear how this is drawn upon to coordinate changing morphological features and ecological demands throughout the sponge life cycle. Results To identify genome-wide events that accompany the pelagobenthic transition in A. queenslandica, we compared global gene expression profiles at four key developmental stages by sequencing the poly(A transcriptome using SOLiD technology. Large-scale changes in transcription were observed as sponge larvae settled on the benthos and began metamorphosis. Although previous systematics suggest that the only clear homology between Porifera and other animals is in the embryonic and larval stages, we observed extensive use of genes involved in metazoan-associated cellular processes throughout the sponge life cycle. Sponge-specific transcripts are not over-represented in the morphologically distinct adult; rather, many genes that encode typical metazoan features, such as cell adhesion and immunity, are upregulated. Our analysis further revealed gene families with candidate roles in competence, settlement, and metamorphosis in the sponge, including transcription factors, G-protein coupled receptors and other signaling molecules. Conclusions This first genome-wide study of the developmental transcriptome in an early branching metazoan highlights major transcriptional events that accompany the pelagobenthic transition and point to a network of regulatory mechanisms that coordinate changes in morphology with

  12. Comparison of the bacterial species diversity of spontaneous cocoa bean fermentations carried out at selected farms in Ivory Coast and Brazil.

    Science.gov (United States)

    Papalexandratou, Zoi; Camu, Nicholas; Falony, Gwen; De Vuyst, Luc

    2011-08-01

    To compare the spontaneous cocoa bean fermentation process carried out in different cocoa-producing regions, heap and box (one Ivorian farm) and box (two Brazilian farms) fermentations were carried out. All fermentations were studied through a multiphasic approach. In general, the temperature inside the fermenting mass increased throughout all fermentations and reached end-values of 42-48 °C. The main end-products of pulp carbohydrate catabolism were ethanol, lactic acid, acetic acid, and/or mannitol. In the case of the fermentations on the selected Ivorian farm, the species diversity of lactic acid bacteria (LAB) and acetic acid bacteria (AAB) was restricted. Lactobacillus fermentum and Leuconostoc pseudomesenteroides were the predominant LAB species, due to their ethanol and acid tolerance and citrate consumption. The levels of mannitol, ascribed to growth of L. fermentum, were fermentation-dependent. Also, enterobacterial species, such as Erwinia soli and Pantoea sp., were among the predominating microbiota during the early stages of both heap and box fermentations in Ivory Coast, which could be responsible for gluconic acid production. Consumption of gluconic acid at the initial phases of the Ivorian fermentations could be due to yeast growth. A wider microbial species diversity throughout the fermentation process was seen in the case of the box fermentations on the selected Brazilian farms, which differed, amongst other factors, regarding pod/bean selection on these farms as compared to fermentations on the selected Ivorian farm. This microbiota included Lactobacillus plantarum, Lactobacillus durianis, L. fermentum, Lactobacillus mali, Lactobacillus nagelii, L. pseudomesenteroides, and Pediococcus acidilactici, as well as Bacillus subtilis that was present at late fermentation, when the temperature inside the fermenting mass reached values higher than 50 °C. Moreover, AAB seemed to dominate the Brazilian box fermentations studied, explaining higher acetic

  13. Hymenobacter perfusus sp. nov., Hymenobacter flocculans sp. nov. and Hymenobacter metalli sp. nov. three new species isolated from an uranium mine waste water treatment system.

    Science.gov (United States)

    Chung, Ana Paula; Lopes, André; Nobre, M Fernanda; Morais, Paula V

    2010-12-01

    Three red-pink pigmented strains, designated A1-12(T), A2-50A(T) and A2-91(T), were recovered from two different sites in a uranium mine. For all strains, the optimum growth temperature was 25°C, the optimum pH was 6.0-6.5 and the DNA G+C contents were between 60 and 63.4 mol%. The major respiratory quinone was menaquinone 7 (MK-7) and the fatty acid profiles contained iso- and anteiso-branched C15 fatty acids, summed feature 3 (16:1 ω6c and/or ω7c and/or 15:0 iso 2-OH), summed feature 4 (17:1 anteiso B and/or iso I) and the unsaturated fatty acid 16:1 ω5c as the major components. Phylogenetic analysis of the 16S rRNA gene sequences showed that these organisms represented three distinct branches within the family Flexibacteraceae most closely related to the members of the genus Hymenobacter. Strain A1-12(T) formed a distinct phylogenetic line along with H. rigui KCTC 12533(T) and they shared approximately 98.9% 16S rRNA gene sequence similarity. However, these two strains shared only 14.7% pairwise similarity in their genomic DNA. Strains A2-50A(T) and A2-91(T) formed two distinct lineages, related to the species H. soli KCTC 12607(T), sharing about 95.5% 16S rRNA gene sequence similarity between themselves, and 88.3 and 92.0% with other members of the genus Hymenobacter. Based on the phylogenetic analysis and physiological and biochemical characteristics, these isolates were considered to represent three novel species for which we propose the names Hymenobacter perfusus for strain A1-12(T) (=CIP 110166=LMG 26000), Hymenobacter flocculans for strain A2-50A(T) (=CIP 110139=LMG 25699) and Hymenobacter metalli for strain A2-91(T) (=CIP 110140=LMG 25700). PMID:21051170

  14. Testing an astronomically based decadal-scale empirical harmonic climate model versus the IPCC (2007) general circulation climate models

    Science.gov (United States)

    Scafetta, Nicola

    2012-05-01

    We compare the performance of a recently proposed empirical climate model based on astronomical harmonics against all CMIP3 available general circulation climate models (GCM) used by the IPCC (2007) to interpret the 20th century global surface temperature. The proposed astronomical empirical climate model assumes that the climate is resonating with, or synchronized to a set of natural harmonics that, in previous works (Scafetta, 2010b, 2011b), have been associated to the solar system planetary motion, which is mostly determined by Jupiter and Saturn. We show that the GCMs fail to reproduce the major decadal and multidecadal oscillations found in the global surface temperature record from 1850 to 2011. On the contrary, the proposed harmonic model (which herein uses cycles with 9.1, 10-10.5, 20-21, 60-62 year periods) is found to well reconstruct the observed climate oscillations from 1850 to 2011, and it is shown to be able to forecast the climate oscillations from 1950 to 2011 using the data covering the period 1850-1950, and vice versa. The 9.1-year cycle is shown to be likely related to a decadal Soli/Lunar tidal oscillation, while the 10-10.5, 20-21 and 60-62 year cycles are synchronous to solar and heliospheric planetary oscillations. We show that the IPCC GCM's claim that all warming observed from 1970 to 2000 has been anthropogenically induced is erroneous because of the GCM failure in reconstructing the quasi 20-year and 60-year climatic cycles. Finally, we show how the presence of these large natural cycles can be used to correct the IPCC projected anthropogenic warming trend for the 21st century. By combining this corrected trend with the natural cycles, we show that the temperature may not significantly increase during the next 30 years mostly because of the negative phase of the 60-year cycle. If multisecular natural cycles (which according to some authors have significantly contributed to the observed 1700-2010 warming and may contribute to an

  15. Comprehensive Assessments of RNA-seq by the SEQC Consortium: FDA-Led Efforts Advance Precision Medicine.

    Science.gov (United States)

    Xu, Joshua; Gong, Binsheng; Wu, Leihong; Thakkar, Shraddha; Hong, Huixiao; Tong, Weida

    2016-01-01

    Studies on gene expression in response to therapy have led to the discovery of pharmacogenomics biomarkers and advances in precision medicine. Whole transcriptome sequencing (RNA-seq) is an emerging tool for profiling gene expression and has received wide adoption in the biomedical research community. However, its value in regulatory decision making requires rigorous assessment and consensus between various stakeholders, including the research community, regulatory agencies, and industry. The FDA-led SEquencing Quality Control (SEQC) consortium has made considerable progress in this direction, and is the subject of this review. Specifically, three RNA-seq platforms (Illumina HiSeq, Life Technologies SOLiD, and Roche 454) were extensively evaluated at multiple sites to assess cross-site and cross-platform reproducibility. The results demonstrated that relative gene expression measurements were consistently comparable across labs and platforms, but not so for the measurement of absolute expression levels. As part of the quality evaluation several studies were included to evaluate the utility of RNA-seq in clinical settings and safety assessment. The neuroblastoma study profiled tumor samples from 498 pediatric neuroblastoma patients by both microarray and RNA-seq. RNA-seq offers more utilities than microarray in determining the transcriptomic characteristics of cancer. However, RNA-seq and microarray-based models were comparable in clinical endpoint prediction, even when including additional features unique to RNA-seq beyond gene expression. The toxicogenomics study compared microarray and RNA-seq profiles of the liver samples from rats exposed to 27 different chemicals representing multiple toxicity modes of action. Cross-platform concordance was dependent on chemical treatment and transcript abundance. Though both RNA-seq and microarray are suitable for developing gene expression based predictive models with comparable prediction performance, RNA-seq offers

  16. Il contributo di una Unitá Operativa Complessa: di Infettivologia Pediatrica durante l'epidemia di morbillo in Campania.

    Directory of Open Access Journals (Sweden)

    G. De Lorenzo

    2003-05-01

    Full Text Available

    Il morbillo è un importante problema di Sanità Pubblica che comporta un elevato rischio di complicanze con conseguenti eccessi di ospedalizzazione, mortalità e costi elevati.

    Obiettivi: Valutare incidenza, decorso clinico, complicanze e scelte terapeutiche in bambini ospedalizzati per morbillo, nonché l’impatto dell’epidemia su strutture ed operatori di una U.O.C. di Infettivologia Pediatrica con 16 p.l.

    Metodologia: dalle cartelle cliniche dei bambini ricoverati per morbillo presso l’U.O.C. di Pediatria Infettivologica dell’Ospedale Pausillipon di Napoli, nel corso dell’epidemia verificatasi in Campania tra gennaio e maggio 2002, sono stati raccolti, mediante una apposita griglia di rilevazione, i dati socio anagrafici, quelli relativi a decorso della malattia, eventuali complicanze, scelte terapeutiche adottate, stato immunitario del bambino e della madre, possibile fonte di contagio, motivazione al ricovero. Su 78 bambini ricoverati (38F e 40M, età media di 4,2 anni (range 0,1 14 anni, di cui 20 con età inferiore a 15 mesi, 42 hanno presentato complicanze: 27 broncopolmoniti, di cui 4 di presumibile origine virale e 23 da presumibile sovrainfezione batterica, 7 laringotracheobronchiti (croup, 4 diarrea con disidratazione, 1 otite media, 4 piastrinopenia transitoria, 2 stomatiti, 1 cistite emorragica, 1 meningoencefalite. Oltre alla terapia antibiotica e cortisonica (quando indicate, 27 bambini hanno ricevuto una singola somministrazione di Vitamina A, con decorso favorevole della malattia e nessun effetto collaterale. In soli due bambini era stata praticata la vaccinazione antimorbillo.

    Conclusioni: i dati confermano l’importanza della vaccinazione antimorbillo e l’opportunità di anticiparla a 6 mesi in corso di epidemia (nella quasi totalità dei casi di età ‹15 mesi la fonte di contagio è stata la malattia in

  17. Characterization of a biogas-producing microbial community by short-read next generation DNA sequencing

    Directory of Open Access Journals (Sweden)

    Wirth Roland

    2012-07-01

    Full Text Available Abstract Background Renewable energy production is currently a major issue worldwide. Biogas is a promising renewable energy carrier as the technology of its production combines the elimination of organic waste with the formation of a versatile energy carrier, methane. In consequence of the complexity of the microbial communities and metabolic pathways involved the biotechnology of the microbiological process leading to biogas production is poorly understood. Metagenomic approaches are suitable means of addressing related questions. In the present work a novel high-throughput technique was tested for its benefits in resolving the functional and taxonomical complexity of such microbial consortia. Results It was demonstrated that the extremely parallel SOLiD™ short-read DNA sequencing platform is capable of providing sufficient useful information to decipher the systematic and functional contexts within a biogas-producing community. Although this technology has not been employed to address such problems previously, the data obtained compare well with those from similar high-throughput approaches such as 454-pyrosequencing GS FLX or Titanium. The predominant microbes contributing to the decomposition of organic matter include members of the Eubacteria, class Clostridia, order Clostridiales, family Clostridiaceae. Bacteria belonging in other systematic groups contribute to the diversity of the microbial consortium. Archaea comprise a remarkably small minority in this community, given their crucial role in biogas production. Among the Archaea, the predominant order is the Methanomicrobiales and the most abundant species is Methanoculleus marisnigri. The Methanomicrobiales are hydrogenotrophic methanogens. Besides corroborating earlier findings on the significance of the contribution of the Clostridia to organic substrate decomposition, the results demonstrate the importance of the metabolism of hydrogen within the biogas producing microbial

  18. Origins of a 350-kilobase genomic duplication in Mycobacterium tuberculosis and its impact on virulence.

    Science.gov (United States)

    Domenech, Pilar; Rog, Anya; Moolji, Jalal-ud-din; Radomski, Nicolas; Fallow, Ashley; Leon-Solis, Lizbel; Bowes, Julia; Behr, Marcel A; Reed, Michael B

    2014-07-01

    In the present study, we have investigated the evolution and impact on virulence of a 350-kb genomic duplication present in the most recently evolved members of the Mycobacterium tuberculosis East Asian lineage. In a mouse model of infection, comparing HN878 subclones HN878-27 (no duplication) and HN878-45 (with the 350-kb duplication) revealed that the latter is impaired for in vivo growth during the initial 3 weeks of infection. Furthermore, the median survival time of mice infected with isolate HN878-45 is significantly longer (77 days) than that of mice infected with HN878-27. Whole-genome sequencing of both isolates failed to reveal any mutational events other than the duplication that could account for such a substantial difference in virulence. Although we and others had previously speculated that the 350-kb duplication arose in response to some form of host-applied selective pressure (P. Domenech, G. S. Kolly, L. Leon-Solis, A. Fallow, M. B. Reed, J. Bacteriol. 192: 4562-4570, 2010, and B. Weiner, J. Gomez, T. C. Victor, R. M. Warren, A. Sloutsky, B. B. Plikaytis, J. E. Posey, P. D. van Helden, N. C. Gey van Pittius, M. Koehrsen, P. Sisk, C. Stolte, J. White, S. Gagneux, B. Birren, D. Hung, M. Murray, J. Galagan, PLoS One 7: e26038, 2012), here we show that this large chromosomal amplification event is very rapidly selected within standard in vitro broth cultures in a range of isolates. Indeed, subclones harboring the duplication were detectable after just five rounds of in vitro passage. In contrast, the duplication appears to be highly unstable in vivo and is negatively selected during the later stages of infection in mice. We believe that the rapid in vitro evolution of M. tuberculosis is an underappreciated aspect of its biology that is often ignored, despite the fact that it has the potential to confound the data and conclusions arising from comparative studies of isolates at both the genotypic and phenotypic levels. PMID:24778110

  19. Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.

    Directory of Open Access Journals (Sweden)

    Honghui Zhu

    Full Text Available Heavy-metal-tolerant bacteria, GIMN1.004(T, was isolated from mine soils of Dabaoshan in South China, which were acidic (pH 2-4 and polluted with heavy metals. The isolation was Gram-negative, aerobic, non-spore-forming, and rod-shaped bacteria having a cellular width of 0.5-0.6 µm and a length of 1.3-1.8 µm. They showed a normal growth pattern at pH 4.0-9.0 in a temperature ranging from 5 °C to 40 °C.The organism contained ubiquinone Q-8 as the predominant isoprenoid quinine, and C(16:0, summed feature 8 (C(18:1ω7c and C(18:1ω6c, C(18:0, summed feature 3 (C(16:1ω7c or iso-C(15:0 2-OH, C(17:0 cyclo, C(18:1ω9c, C(19:0 cyclo ω8c, C(14:0 as major fatty acid. These profiles were similar to those reported for Burkholderia species. The DNA G+C % of this strain was 61.6%. Based on the similarity to 16S rRNA gene sequence, GIMN1.004(T was considered to be in the genus Burkholderia. The similarities of 16S rRNA gene sequence between strain GIMN1.004(T and members of the genus Burkholderia were 96-99.4%, indicating that this novel strain was phylogenetically related to members of that genus. The novel strain showed the highest sequence similarities to Burkholderia soli DSM 18235(T (99.4%; Levels of DNA-DNA hybridization with DSM 18235(T was 25%. Physiological and biochemical tests including cell wall composition analysis, differentiated phenotype of this strain from that closely related Burkholderia species. The isolation had great tolerance to cadmium with MIC of 22 mmol/L, and adsorbability of 144.94 mg/g cadmium,and it was found to exhibit antibiotic resistance characteristics. The adsorptive mechanism of GIMN1.004(T for cadmium depended on the action of the amide,carboxy and phosphate of cell surface and producing low-molecular-weight (LMW organic acids to complex or chelated Cd(2+.Therefore, the strain GIMN1.004(T represented a new cadmium resistance species, which was tentatively named as Burkholderia dabaoshanensis sp. nov. The strain type

  20. Adaptedd Winkler model based on nonlinear dynamic analysis of soil-pile%基于桩-土非线性动力分析改进的Winkler模型

    Institute of Scientific and Technical Information of China (English)

    侯元恒

    2012-01-01

    一种数值模型用来研究地震作用下桩基础反应,在Winkler模型基础上,考虑了桩的柔性与能量耗散及辐射.模型用来研究桩-土-结构共同作用分析,包括桩土非线性效应,考虑桩土体系非线性及其在强震作用下会产生大的变形,桩采用材料与几何非线性的梁-柱元相同,而土体假想为沿桩长段均匀的非线性弹簧-阻尼器,进而采用修正的Ramberg-Osgood模型.利用剪切模量与阻尼一个关系导得土的阻尼.为了模拟土中辐射阻尼效应,桩与土采用粘性阻尼器连接.模型同文献中的振动台测试对比表明,该模型与测试结果较吻合,进而为强震作用下桩土分析提供一种数值分析方法.%A numerical model to study the seismic response of pile foundation,it is based on Winkler model,takes into account both pile flexibility and energy dissipation and radiation. The model is applied to study the influence of soil-pile-structure interaction on the response of structures,including nonlinear pile-soil effects. To incorporate nonlinearities in the soil-pile system might arise from large deformations under severe earthquake loading,the piles' material and geometry using non-linear the beam-column element, while the soil is idealized by nonlinear springs and dashpots uniformly attached to the piles along their length. For the soli springs, a modified Ramberg-Osgood model is employed;for the material damping of the soil,a simple relationship between the shear modulus and damping is utilized. To simulate the radiation damping effect in the soil, viscous dampers are attached to the pile. Model is compared with the literature of shaking table tests,indicating that the model agrees with the test results,and provides a numerical analysis for the strong motion of pile-soil analysis methods.

  1. Music and the Nature: Input of the Czech Composers

    Science.gov (United States)

    Nemec, Vaclav; Nemcova, Lidmila

    2014-05-01

    Slovak mountains. Josef Suk (1874 - 1935): Pohádka léta (A Summer's Tale) - a work for orchestra remembering a relax and consolation in beauties of the Nature. Bohuslav Martinů (1890 - 1959): Otvírání studánek (The Opening of the Springs) - cantata for soli, female chorus and instrumental accompaniment remembering old local customs of purifying water in the wells when spring comes.

  2. Unusual Polar Activity of the Sun in the Northern Hemisphere and Its Implications for Solar Cycle 25

    Science.gov (United States)

    Gopalswamy, Nat; Masuda, Satoshi; Yashiro, Seiji; Akiyama, Sachiko; Shibasaki, Kiyoto

    2016-07-01

    Polar field strength in one solar cycle is known to indicate the strength (e.g., Sunspot number) and phase of the next cycle. In particular the polar field strength (or its proxies such as the polar coronal hole area and microwave polar brightness) during the minimum phase of a given cycle seem to be well correlated with the maximum sunspot number of the next cycle. Polar prominence eruptions and coronal mass ejections have also been found to be indicators of low polar field; their cessation signals the time of polarity reversal. While these indicators are present in the current cycle, significant differences are found regarding the phase lag between the two hemispheres and the duration of polar eruptions. We use data from the Nobeyama Radioheliograph, the Solar Dynamics Observatory, SOLIS, and Wilcox Solar Observatory to highlight these differences. We find that the north polar region of the Sun has near-zero field strength for more than three years. This is unusually long and caused by surges of both polarities heading toward the north pole that prevent the buildup of the polar field. This seems to be due to anti-Hale active regions that appeared around the 2012 peak sunspot activity in the northern hemisphere. The unusual condition is consistent with (i) the continued high-latitude prominence eruption, (ii) the extended period of high tilt angle of the heliospheric current sheet, (iii) the weak microwave polar brightness, and (iv) the lack of north polar coronal hole. On the other hand, the south polar field has started building up and the coronal hole has appeared in early 2015 because of large active regions of the correct tilt in the southern hemisphere during the 2014 peak of sunspot activity. The extended period of near-zero field in the north polar region should result in very weak and delayed sunspot activity in the northern hemisphere in cycle 25. On the other hand the south polar field has already increased significantly, suggesting that the activity in

  3. Whole genome resequencing of black Angus and Holstein cattle for SNP and CNV discovery

    Directory of Open Access Journals (Sweden)

    Stothard Paul

    2011-11-01

    Full Text Available Abstract Background One of the goals of livestock genomics research is to identify the genetic differences responsible for variation in phenotypic traits, particularly those of economic importance. Characterizing the genetic variation in livestock species is an important step towards linking genes or genomic regions with phenotypes. The completion of the bovine genome sequence and recent advances in DNA sequencing technology allow for in-depth characterization of the genetic variations present in cattle. Here we describe the whole-genome resequencing of two Bos taurus bulls from distinct breeds for the purpose of identifying and annotating novel forms of genetic variation in cattle. Results The genomes of a Black Angus bull and a Holstein bull were sequenced to 22-fold and 19-fold coverage, respectively, using the ABI SOLiD system. Comparisons of the sequences with the Btau4.0 reference assembly yielded 7 million single nucleotide polymorphisms (SNPs, 24% of which were identified in both animals. Of the total SNPs found in Holstein, Black Angus, and in both animals, 81%, 81%, and 75% respectively are novel. In-depth annotations of the data identified more than 16 thousand distinct non-synonymous SNPs (85% novel between the two datasets. Alignments between the SNP-altered proteins and orthologues from numerous species indicate that many of the SNPs alter well-conserved amino acids. Several SNPs predicted to create or remove stop codons were also found. A comparison between the sequencing SNPs and genotyping results from the BovineHD high-density genotyping chip indicates a detection rate of 91% for homozygous SNPs and 81% for heterozygous SNPs. The false positive rate is estimated to be about 2% for both the Black Angus and Holstein SNP sets, based on follow-up genotyping of 422 and 427 SNPs, respectively. Comparisons of read depth between the two bulls along the reference assembly identified 790 putative copy-number variations (CNVs. Ten

  4. Avaliação de parâmetros hidráulicos e manejo da irrigação por microaspersão em área de assentamento Hydraulic parameters evaluation and management of microsprinkler irrigation in settlement area

    Directory of Open Access Journals (Sweden)

    Fábio S. Santiago

    2004-12-01

    Full Text Available Este trabalho foi desenvolvido em solo aluvial do semi-árido de Pernambuco, suscetível à salinização, visando a orientar agricultores de assentamento para instalar, avaliar e manejar racionalmente sistemas de irrigação por microaspersão, para atender ao plantio da cultivar de repolho Midori. Adotando-se percentagem de área molhada igual a 100%, foram avaliados nos setores da unidade operacional a variação de vazão e pressão, os coeficientes de uniformidade (CU e a eficiência de aplicação (EA. A uniformidade da irrigação apresentou valores elevados, superiores a 93%. A eficiência de aplicação foi boa, com valores maiores que 80%. O sistema foi então utilizado na irrigação, utilizando como controle o tanque "Classe A", durante um período de 75 dias, e conduzido por um agricultor local. Considerando os riscos de salinização na área, promoveu-se lavagem contínua do solo. Foi adotado um coeficiente de lixiviação médio de 1,21, com desvio-padrão de 0,45, para o período sem precipitação. Praticamente em todo o experimento, o sentido do gradiente de potencial total da água do solo foi ascendente, com média de 1,14 e 0,21 de desvio-padrão. Preveniu-se com isso a ocorrência de ascensões capilares, estabelecendo-se um perfil drenante, bem como se manteve a condutividade elétrica da zona saturada em equilíbrio. Não foi observada a saturação do solo na zona radicular.This study has been carried out in alluvial area in the semi-arid zone of Pernambuco State, Brazil, subject to salinisation, aiming to guide local farmers to install, evaluate and to manage micro sprinkler irrigation systems, raising Midori cabbage cultivar. Adopting the area percentage of wetted soli 100% the sub units have been evaluated regarding the discharge and pressure variation, and also coefficients of uniformity (CU and efficiency of application coefficient (EA. The irrigation uniformity was high, greater than 93%. The efficiency of

  5. Next Generation Sequencing and Its Application in Forensic Genetics%二代测序技术及其在法医遗传学中的应用

    Institute of Scientific and Technical Information of China (English)

    王乐; 叶健; 白雪; 杨帆; 赵兴春

    2015-01-01

    analyzing the STR, SNP, Indel and RNA markers simultaneously, which could be impossible on routinely used PCR-CE platforms because of the limited amount of exhibits. In this article, the authors attempt to describe the basic concepts, developmental history and working principles of NGS to Chinese experts in the general field of forensic science and technologies, and share the updates of NGS-based STR typing, SNP typing and whole mtGenome sequencing during the past two years. Representative NGS platforms including the 454/Roche GS FLX system, the Solexa system, the SOLiDTM system, the Ion PGMTM system and the MiSeq FGxTM system were introduced. Annual statistics of research articles on NGS and forensic NGS were described and trends for related research were analyzed. Finally, perspectives of forensic NGS were presented and possible challenges including data analysis methods, openness of NGS systems and ethical issues were discussed in the hope of providing a reference for related research and applications.

  6. Transcriptome sequencing for SNP discovery across Cucumis melo

    Directory of Open Access Journals (Sweden)

    Blanca José

    2012-06-01

    Full Text Available Abstract Background Melon (Cucumis melo L. is a highly diverse species that is cultivated worldwide. Recent advances in massively parallel sequencing have begun to allow the study of nucleotide diversity in this species. The Sanger method combined with medium-throughput 454 technology were used in a previous study to analyze the genetic diversity of germplasm representing 3 botanical varieties, yielding a collection of about 40,000 SNPs distributed in 14,000 unigenes. However, the usefulness of this resource is limited as the sequenced genotypes do not represent the whole diversity of the species, which is divided into two subspecies with many botanical varieties variable in plant, flowering, and fruit traits, as well as in stress response. As a first step to extensively document levels and patterns of nucleotide variability across the species, we used the high-throughput SOLiD™ system to resequence the transcriptomes of a set of 67 genotypes that had previously been selected from a core collection representing the extant variation of the entire species. Results The deep transcriptome resequencing of all of the genotypes, grouped into 8 pools (wild African agrestis, Asian agrestis and acidulus, exotic Far Eastern conomon, Indian momordica and Asian dudaim and flexuosus, commercial cantalupensis, subsp. melo Asian and European landraces, Spanish inodorus landraces, and Piel de Sapo breeding lines yielded about 300 M reads. Short reads were mapped to the recently generated draft genome assembly of the DHL line Piel de Sapo (inodorus x Songwhan Charmi (conomon and to a new version of melon transcriptome. Regions with at least 6X coverage were used in SNV calling, generating a melon collection with 303,883 variants. These SNVs were dispersed across the entire C. melo genome, and distributed in 15,064 annotated genes. The number and variability of in silico SNVs differed considerably between pools. Our finding of higher genomic diversity in wild

  7. LE STAZIONI DI APPRENDIMENTO: ESEMPI DIDATTICI PER UN APPROCCIO DI TIPO GLOBALE ALL’INSEGNAMENTO DELLA LINGUA ITALIANA A STRANIERI

    Directory of Open Access Journals (Sweden)

    Antonietta Gobbis

    2015-02-01

    Full Text Available Il metodo, che si rifà alle teorie pedagogiche di Maria Montessori (1870-1952, è un esempio di lezione “aperta” fondato su pratiche che attivano la sfera emozionale, sensoriale e relazionale oltre a quella logico-razionale della mente. Nel processo di apprendimento di conoscenze acquisite non solo attraverso l’ascolto delle parole dell’insegnante, ma anche attraverso esperienze scelte autonomamente e consapevolmente da soli e in gruppo, l’ambiente delle stazioni didattiche offre tutto il materiale di sviluppo cognitivo in maniera che sia liberamente fruibile dal discente per favorirne la socializzazione e l’autonomia (secondo il motto di Maria Montessori ‘Aiutami a fare da solo’. A lezione esse non solo offrono varietà, comunicazione e divertimento ma, e in ciò è il loro massimo pregio, differenziazione interna al gruppo degli studenti, che possono, senza alcuna pressione da parte dei compagni, lavorare al proprio ritmo e livello scegliendogli esercizi secondo le preferenze individuali lungo un percorso aperto a canali diversi di apprendimento e orientato ad un vivace interscambio con gli altri.Il nostro contributo fa riferimento a tre esempi di stazioni didattiche legate alle feste che più richiamano l’attenzione degli studenti stranieri sul nostro paese: il Natale e il Carnevale. Tali tematiche mostrano come il modello della stazioni didattiche riesca a rapportarsi al vissuto degli studenti motivandoli al raffronto interculturale. Le abbiamo sperimentate in gruppi di studenti dell’Università delle Lingue Mondiali di Tashkent che frequentano il primo e secondo anno del corso di Italiano, studiato come seconda lingua.   Learning stations: didactic examples for a global teaching method This method, based on Maria Montessori’s educational theories, is an example of “open” lessons based on activities that activate emotions, senses and relationships, besides logic and reason. In the learning process knowledge is

  8. Bioaccumulation of Zn and Ag Nanoparticles in the Earthworms (Eisenia fetida)

    Science.gov (United States)

    Ha, Lee Seung; Sung-Dae, Kim; Yi, Yang Song; Byeong-Gweon, Lee

    2014-05-01

    present mainly in HSP (heat-sensitive protein) while cellullar Ag from Ag ion and AgNPs (Ag/PVP, Ag/citrate) treatments were found mostly in cellular debris. No statistical difference in partitioning of metals among different subcelluar pools was found between the metal forms. Zn from ZnO contaminated solis was found largely in carbonate fraction (41%), while Zn from Zn ion treatment was found in Fe-Mn Oxide (29%). Association of Zn to mobile fractions (ZnO; 65%, Zn ion; 35%) suggest that Zn from ZnO contaminated soil would be more bioavailable than that from Zn ion treatment. However, the BAFs for Zn in the animals did not follow this prediction. Majority of Ag from AgNPs or Ag ion contaminated soil was bound mainly to biologically inert fractions mainly in organic matter, surphide fractions, and residual fractions. Consistent with these findings, the BAFs of Ag in the worms exposed to Ag contaminated soils were generally lower than those for Zn treatments.

  9. Editorial

    Directory of Open Access Journals (Sweden)

    Javier Hernández-Fernández

    2015-12-01

    rapidez y bajado los costos exponencialmente. Tres tecnologías de NGS son utilizadas masivamente, aunque ya hay disponibles otros métodos que no son igualmente utilizados. 454 GSFlex de Roche, Genome Analyzer o HiSeq de Illumina y SOLiD de Life Technologies que con sus mejoras y su bajo costo han permitido en casi todo el planeta dar inicio a un número inusitado de proyectos que en últimas buscan inventariar todos los genes del mundo. Recientemente el mercado ofrece versiones de equipos NGS con características un poco limitadas en rendimiento y capacidad de secuenciación, pero más fácilmente utilizables por la comunidad de investigadores, y, además, orientados a un segmento de mercado diferente. Entre ellos tenemos: los equipos 454 GS Junior de Roche, MiSeq Personal Sequencer de Illumina e Ion Torrent de Life Technologies

  10. Phytochemical and biological evaluation of Preskia bleo (Kunth) DC, a plant species used in traditional medicine for the treatment of diabetes

    International Nuclear Information System (INIS)

    The leaves of Pereskia bleo (pokok jarum tujuh), are being used in Malaysian traditional medicine for the prevention and treatment of breast cancer. In order to validate the use of this plant in breast cancer treatment we have prepared crude extracts of the leaves and assessed them for activity against several breast cancer cell lines such as the MCF-7, MDA-MB231 and against non-tumour 3T3 cells. The crude extracts were also subjected to toxicity test using brine shrimp lethality assay. Dried and milled Pereskia bleo leaves were extracted successively with petroleum ether, chloroform, methanol and distilled water. Extracts of the organic solvent were filtered and the supernatant were concentrated using the rotary evaporator. The aqueous extract was lyophilized. The extracts were screened for the presence of alkaloids, terpenoids, flavanoids and saponins. Activity against Artemia salina was determined using a 96-well microplate method (Solis et al., 1993). Inhibition of growth of cultured cancer cell lines were measured using a standard MTT assay. Cytotoxic drugs, tamoxifen, was used as a positive control against the brine shrimps and cell lines, respectively. All the non-polar extracts of the leaves were found to exhibit significant cytotoxic activities against both the ER positive (MCF7) and ER negative (MDA-MB231) breast cancer cell-lines but. All the extracts were found to be not cytotoxic against the non-tumour (3T3) cells. The non-polar extracts were found to be 2 to 3 times more cytotoxic than the more polar methanol extracts against the ER positive, MCF-7 cells. The strongest cytotoxic activity was observed with petroleum extract with IC50 values of 6.5 μg/ml and 11.5 μg/ml towards MCF-7 and MDA-MB cell lines, respectively. The cytotoxic activities of the petroleum ether and chloroform extracts against the ER negative, MDA-MB231 cell-line are about the same (IC50 19.3 to 26.3 μg/ml ). The cytotoxic activities of the anticancer drug, tamoxifen, against the

  11. Nachweis von Zytomegalie-Virus Immunglobulin G und M Antikrpernaus filtergetrocknetem Neugeborenenblut

    Institute of Scientific and Technical Information of China (English)

    QIAN; Xiaohu

    2001-01-01

    [1]Fowler K B, Stagno S, Pass R F et al. The outcome of congenital cytomegalovirus infection in relation to maternal antibody status. N Engl J Med, 1992,326:663[2]Evengard B, Linder E. Standardization of a filter-paper technique for blood sampling. Ann Trop Med Parasitol,1998,82:295[3]Wassilak S G F, Bernier R H, Herrmann K L et al.Measles seroconfirmation using dried blood specimens on filter paper. Pediatr Infect Dis, 1984,3:117[4]Lebech M, Petersen E. Detection by enzyme immunosorbent assay of Toxoplasma gondii IgG antibodies in dried blood spots on PKU-filter paper from newborn. Scand J Infect Dis, 1995,27: 259[5]Lamy M E, Mulongo K N, Gadisseux J F et al. Prenatal diagnosis of fetal cytomegalovirus infection. Am J Obstet Gynecol, 1992,166 : 91[6]Takkouche B, Iglesias J, Alonso-Fernandez J R et al. Detection of Brucella antibodies in eluted dried blood: a validation study. Immunol Lett, 1995,45:107[7]Matsubara K, Tanabe K, Akane A et al. A novel assay for typing Rh antigens in blood-stains using a lectin specific to the bisecting N- acetyl-D-glucosamine side chain of glycoprotein. J Immunol Methods, 1994,173:175[8]Pappaioanou M, Kashamuka M, Behets F et al. Accurate detection of maternal antibodies to HIV in newborn whole blood dried on filter paper. AIDS, 1993,7:483[9]Major C J, Read S E, Francis R A et al. Comparison of saliva and blood for human immunodeficiency virus prevalence testing. J Infect Dis, 1991,163:699[10]Gil A, Gonzalez A, Dominquez V et al. Detection of antibodies against hepatitis A in blood spots dried on filter paper. Is this a reliable method for epidemiological studies ? Epidemiol-Infect, 1997,118:189[11]Torrela A, Solis R L, Rodriguez N et al. Ultramicro ELISA to the detection of IgM antibodies in Mycobacterium leprae using dry blood samples. Rev Inst Med Trop Sao Paulo, 1994,36:131[12]Stevens R, Pass K, Fuller S et al. Blood spot screening and confirmation tests for Syphilis antibody. J

  12. Soil Wind Erosion Risk Assessment in Yulin City Using T-S Fuzzy Neural Network Model%基于T-S模糊神经网络模型的榆林市土壤风蚀危险度评价

    Institute of Scientific and Technical Information of China (English)

    刘新颜; 曹晓仪; 董治宝

    2013-01-01

    Soli wind erosion,one of the major causes of environmental problems such as farmland degradation and desertification,producing large scale of sandstorm,polluting the environment and harming human beings' health in arid and semi-arid regions,is the detachment,entrainment,transport deposition of soil particles caused by wind.It is also a serious socio-economic issue which limits the development in those ecologically fragile areas.In the previous studies,methods for accessing wind erosion are based on land use information.In this article,however,another approach that uses GIS and T-S fuzzy neural network model to access soil wind erosion risk is presented.The approach is applied to a case study of the Yulin City located on the wind-sand transition regions of Mu Us sandy land,Inner Mongolian plateau and loess plateau,northwest China,where most serious expansion of desertification is proceeding.Environmental elements of soil wind erosion were selected and their spatial distribution data were extracted using GIS technology and T-S fuzzy neural network model was constructed after training the sample data of different wind erosion risk level.The model was applied to mapping wind erosion risk after inputting the extracted date.The entire jobs were based on the software of ArcGIS9.3 and MATLAB.The results show that:Firstly,the comparison of the risk assessment with land use information in Yulin City demonstrates that the result is in conformity with actual conditions.T-S fuzzy neural network model can reveal the relationship between risk of soil wind erosion and its environment effectively,and provide the basis for predicting wind erosion.Secondly,environmental elements such as wind energy,vegetation coverage,temperature,precipitation,topography control the distribution properties of wind erosion risk in Yulin City.Thirdly,there are 4 divisions of wind erosion risk in Yulin City:the severe risk level,the intense risk level,the moderate risk level and the slight risk level

  13. 晶态Li4 Ti5 O12负极材料的制备及其与电解液的兼容性研究%Preparation of crystalline Li4 Ti5 O12 anode material and study on its compatibility with electrolyte

    Institute of Scientific and Technical Information of China (English)

    刘坚; 江民华; 孔青荣

    2014-01-01

    Lithium acetate and butyl titanate were used as raw materials,glacial acetic acid as inhibitor,nanocrystalline Li4 Ti5 O12 for the anode material of lithium-ion battery was prepared by sol-gel technique. The Li4 Ti5 O12 , three pre-prepared electrolytes and a conventional electrolyte were used to assemble four half-cells Li/Li4 Ti5 O12 , respectively. In order to explore the compatibles of Li4 Ti5 O12 toward the electrolytes, we performed the characterizations of galvanostatic charge-discharge tests, cyclic voltammetry (CV)and electrochemical impedance spectroscopy(EIS)for those half-cells. The research results showed that the performances of half-cell Li/Li4 Ti5 O12 could been improved by adding lithium bis( oxalato) borate( LiBOB) to the electrolyte that consisted of LiPF6, cyclic carbonates(EC,PC),and linear carbonate(MEC). The first discharge specific capacity of the half-cell reached 198mA. h. g-1 with less decrease under 20-time cycles of charge-discharge. However,when a little ethylene carbonate was added to the over elec-trolyte,the performances of the half-cell exhibited reduction. So,Li4 Ti5 O12 showed an obvious dependency on electrolyte.%本文以醋酸锂和钛酸丁酯为原料,以冰醋酸为抑制剂,采用溶胶-凝胶法制备了晶态Li4 Ti5 O12负极材料。与自制的3种电解液和实验室常用的电解液分别组装成锂/钛酸锂半电池。采用恒流充放电测试、循环伏安法(CV)及交流阻抗法(EIS)对其电化学性能进行研究。研究结果发现:在以环状碳酸酯类(EC、PC)和线性碳酸酯类( MEC)为溶剂、以六氟磷酸锂( LiPF6)为电解质的电解液中添加双乙二酸硼酸锂( LiBOB),有利于提高半电池的性能,首次放电比电容达到了198mA. h. g-1,且放电比电容经多次充放电后衰减得较小。而在电解液中加入碳酸亚乙烯酯( VC),半电池的性能有所下降。 Li4 Ti5 O12对电解液表现出较明显的兼容性。

  14. Discussion on climate oscillations: CMIP5 general circulation models versus a semi-empirical harmonic model based on astronomical cycles

    Science.gov (United States)

    Scafetta, Nicola

    2013-11-01

    Power spectra of global surface temperature (GST) records (available since 1850) reveal major periodicities at about 9.1, 10-11, 19-22 and 59-62 years. Equivalent oscillations are found in numerous multisecular paleoclimatic records. The Coupled Model Intercomparison Project 5 (CMIP5) general circulation models (GCMs), to be used in the IPCC Fifth Assessment Report (AR5, 2013), are analyzed and found not able to reconstruct this variability. In particular, from 2000 to 2013.5 a GST plateau is observed while the GCMs predicted a warming rate of about 2 °C/century. In contrast, the hypothesis that the climate is regulated by specific natural oscillations more accurately fits the GST records at multiple time scales. For example, a quasi 60-year natural oscillation simultaneously explains the 1850-1880, 1910-1940 and 1970-2000 warming periods, the 1880-1910 and 1940-1970 cooling periods and the post 2000 GST plateau. This hypothesis implies that about 50% of the ~ 0.5 °C global surface warming observed from 1970 to 2000 was due to natural oscillations of the climate system, not to anthropogenic forcing as modeled by the CMIP3 and CMIP5 GCMs. Consequently, the climate sensitivity to CO2 doubling should be reduced by half, for example from the 2.0-4.5 °C range (as claimed by the IPCC, 2007) to 1.0-2.3 °C with a likely median of ~ 1.5 °C instead of ~ 3.0 °C. Also modern paleoclimatic temperature reconstructions showing a larger preindustrial variability than the hockey-stick shaped temperature reconstructions developed in early 2000 imply a weaker anthropogenic effect and a stronger solar contribution to climatic changes. The observed natural oscillations could be driven by astronomical forcings. The ~ 9.1 year oscillation appears to be a combination of long soli-lunar tidal oscillations, while quasi 10-11, 20 and 60 year oscillations are typically found among major solar and heliospheric oscillations driven mostly by Jupiter and Saturn movements. Solar models based

  15. Effect of Solidago canadensis invasions on soil nematode communities in Hangzhou Bay%加拿大一枝黄花入侵对杭州湾地区土壤线虫群落的影响

    Institute of Scientific and Technical Information of China (English)

    许湘琴; 王莹莹; 陆强; 林植华; 陈慧丽

    2011-01-01

    近年来随着地下生态学的发展,生态学家们逐渐重视生物入侵导致的地下生物多样性及其相关生态系统功能的改变.为探究加拿大一枝黄花(Solidago canadensis)入侵对土壤线虫的影响,我们在杭州湾地区选取镇海、平湖、慈溪、奉贤、海盐和杭州6个研究地点,比较外来种加拿大一枝黄花群落与土著种芦苇(Phragmites australis)群落土壤中的线虫群落结构和功能.结果显示:两种植物群落土壤中的线虫属数和多样性没有显著差异,而线虫营养多样性、大多数营养类群百分比以及线虫群落结构均存在显著差异,表明加拿大一枝黄花的入侵在一定程度上改变了杭州湾地区土壤线虫的群落结构;加拿大一枝黄花群落中土壤食真菌线虫比例趋于增加,植食性线虫比例降低,表明加拿大一枝黄花的入侵可能对土壤生态系统的能流途径产生影响,在入侵地抗寄生线虫能力强于土著植物芦苇;采样地点与植物的交互作用对线虫多样性和群落结构的影响显著,表明入侵地点是决定加拿大一枝黄花对土壤生物影响的重要因素之一;土壤颗粒组成、碳含量和氮含量是影响线虫群落结构的主要环境因子.%Ecologists are increasingly focusing on the effects of biological invasions on soil biodiversity and ecosystem functioning. We compared soil nematodes under plant communities dominated by invasive Soli-dago canadensis and native plant Phragmites australis at 6 sites (Zhenhai, Fengxian, Pinghu, Haiyan, Cixi and Hangzhou) in Hangzhou Bay to assess the effects of 5. Canadensis invasions on soil nematode communities. The total number of genera and diversity of soil nematode did not differ between S. Canadensis and P. Australis communities. Trophic diversity, most of the proportions of trophic groups and nematode community structure varied between the two plant communities. These results suggested that the invasion of S

  16. Should LSP Dictionaries. also Include Professional Jargon and Slang?

    Directory of Open Access Journals (Sweden)

    Rosemarie Gläser

    2011-10-01

    NAME, CONNOTATION, STYLISTIC LEVEL/LEVEL OF STYLE, STYLE MARKER, USAGE LABEL, MONOLINGUAL/BILINGUAL DICTIONARY, SOCIOLINGUISTIC STRATIFICATION OF THE SPEECH COMMUNITY

    Zusammenfassung: Sollen Fachworterbiicher auch Berufsjargon und Slangeinbeziehen? Der Artike1 erortert das Problem, ob ein Fachworterbuch auch die soziolinguistischeSchichtung der gesamten Kommunikationsgemeinschaft eines Fachgebietes beriicksichtigen und folgJich neben der Terminologie und Nomenklatur auch den Wortschatz der unteren Stilebenen,wie Berofsjargon und -slang, einbeziehen sollte. Die Autorin geht von der These aus, daBdurch die Einbeziehung dieses Wortschatzes ein Fachworterbuch an Umfang gewinnt, der kommunikativenWirklichkeit starker angenahert ist und speziellen Anforderungen der Obersetzer undDolmetsCher eher gerecht wird. Zur Stiitzung dieser These hat die Autorin zwei e~prachige englischeund fiinf zweisprachige englisch-deutsche (deutsch-englische Fachworterbi.icher untersucht,die zwisChen 1973 und 2000 erschienen sind und den Vorteil haben, daB sie auch Berofsjargon und-slang beriicksichtigen. Die hier vertretenen Fachgebiete reichen von der Rechts·, Wirtschafts- und80rsensprache zur Sprache der Geowissenschaften und der Kraftfahrzeugtechnik. Einige Lexikographenverwenden Stilmarkierungen und SachgebietSindikatoren (z.B. informal, colloquial, slang,vulgar, umgangssprachlich, formlich, gehoben, finance, motoring, press fUr Worter mit stilistischen oderexpressiven Konnotationen. In der Regel sind diese Stilmarkierungen nur bei den ausgangssprachlichenWortem verzeichnet, so daB sie bei dem zielsprachlichen Aquivalent erschlossenwerden miissen. 1m Idealfalle soli ten die Stilmarkiecungen auch bei den zielsprach1ichen Aquivalentenangegeben werden, wofiir einer der Worterbuchautoren den Beweis geliefert hat. ]edochmuG sich der Fachworterbuchautor stets der Tatsache bewuGt sein, daG largon- und Slangworterkurzlebig sein und durch andere umgangssprachliche und plastische Worter aus dem

  17. Fenomen “krov” u sociolingivstici (s romanskim primjerima

    Directory of Open Access Journals (Sweden)

    Žarko Muljačić

    1984-12-01

    tipa (Švicarska Konfederacija, sa svojim kantonima, koji su ponekad dvojezični i višejezidni. Funkcije "krova" obavljaju jezici po izgradjenosti koji mogu biti dominantni ili kodominantni. U prvom slučaju nalazimo "jednostruki krov" a u drugom "složeni krov". Autoi izražava nadu da će slijepo ili u najmanju ruku nedobrovoljno prihvaćanje "krova" (do nedavna na snazi u centralističkim zemljama, sa reperkusijama koje još nisu uklonjene biti zamijenjeno s dobrovoljnim prihvaćanjem "krova", koji u zajedništvu ne smije biti "jednostruk" nego "složen" (jedna viša forma, koju autor naziva "složeni makro-krov", pokriva uostalom tiho i skoro nevidljivo sve evropske jezike, pa čak i one koji nisu članovi indoevropske jezične obitelji, U romanskom svijetu primjer za "složeni krov" je zasigurno Švicarska (nevolje izazvahe ressentimentom stanovništva novoosnovanog kantona Jura su prolazne prirode. Autor se takodjer ukratko osvrće na recentne pojave etnicističkih pokreta koji za "male" jezike po izgradjenosti u nastajanju nisu opasni ako se radi o stanovništvu koje već ima "svoj" jezik po izgradjenosti, nastao na osnovu forme i supstance istog "jezika po udaljenosti" (njem. Abstandsprache, tal. lingua per distanziazione; sit.venia verbo!. Time se misli na jezične planove pijemontskih, venetskih, lombardskih i sicilijanskih te nekih drugih autonomista (na pr. harpitanskih, u autonomnoj regiji Valle d'Aosta: Oni postaju opasni, i čovjek se ne može oteti dojmu da se radi ponekad o manipullianim pokretima po načelu: Divide et imperal, kad se unutar etničkih grupa koje su za dlaku izbjegle lingvicid ili su bile na najboljem putu da mu jednom podlegnu pojave "novi" jezici (kao što je to slučaj s najnovijim razvojem u većinski katalonskoj regiji valencija ili u nekim dijelovima okcitanije.

  18. Romanian Association of Balneology Conference – 2015, 28 – 31 May, Băile Tuşnad

    Directory of Open Access Journals (Sweden)

    MUNTEANU Constantin

    2015-05-01

    Full Text Available 1.\tProf. Onose G, MD, PhD, MSc, Univ. Assist. Haras MA, MD, PhD, Prof. Sinescu CJ, MD, PhD1,2, Univ. Assist. Daia CO, MD, PhD1,2, RDI Assist. Andone I, MD, Postgrad2, Onose VL, MD,3, Assist. Prof. Capisizu A, MD, PhD1,4, Prof. Grigorean VT, MD, PhD1,2, Assoc. Prof. Ciobotaru C, MD, PhD5,6, Sandu AM, MD, Postgrad.2, Assist. Prof. Blendea CD, MD, PhD7, 8 - BASIC WELLNESS FEATURES AND SOME RELATED ACTIONS, PROPENSIVE INCLUDING FOR ACTIVE AND HEALTHY AGEING - 2.\tOlga SURDU, T.V. SURDU, Monica SURDU - STATE OF ART of balneotherapy/thermalisme in Romania 3.\tIliuta Maria Alexandra - THE IMPORTANCE OF NUTRITION IN HEALTH PROGRAMS 4.\tDr. Dogaru Gabriela - THERAPEUTIC EFFECTS OF MINERAL WATERS IN RENAL DISEASES 5.\tVeryho N.S. - THERAPEUTIC EFFECT OF MINERAL WATER WITH DIFFERENT CONCENTRATIONS OF HUMIC ACIDS IN EXPERIMENTAL HEPATITIS INDUCED BY ACETAMINOPHEN 6.\tIoana Marian - SPA SEEN AS AN EXTENSION OF A HEALTH RESORT - A NECESSARY CONDITION FOR INTEGRATING ROMANIA IN THE LIST OF GLOBAL WELLNESS TOURISM DESTINATIONS 7.\tBilha Neli-Claudia, Bilha Stefan - THE BALNEARY TREATMENT COURSE – A BREATH OF LIFE 8.\tBilha Neli-Claudia, Bilha Stefan - WICH SALT MINE DO YOU RECOMMEND FOR SPELEOTHERAPY? Interdisciplinary project proposal. 9.\tGáspár Boróka, Gabriela Dogaru - BALNEOTHERAPY IN THE BOGHIŞ RESORT 10.\tDr. Luminita Aurelia Pasca - REHABILITATION OF ELDERLY PATIENTS WITH CARDIOVASCULAR DISEASE 11.\tDr. Luminita Aurelia Pasca, Dr. Valer Donca - GEROVITAL – PROPHYLAXIS OF AGING AND GERIATRIC TREATMENT 12.\tAndronache Cristina - IMPORTANCE OF REHABILITATION TREATMENT IN HEMOPHILIC ARTHROPATHY – A CASE REPORT 13.\tDr. Motricala Marieta, Molnar Akos - BALNEOTHERAPY IN BAILE TUSNAD 14.\tIoana Pop, Bianca Pop - MEDICAL REHABILITATION IN NEUROPATHIC PAIN 15.\tAmalia Salca¹, Nicoleta Stoica1, Gabriela Dogaru¹,² - BALNEOTHERAPY IN GONARTHROSIS 16.\tAlexandru Bogdan-Cătălin, Toşa Edith-Éva - USING PULSED SHORT WAVE (DIAPULSE COMPLEMENTARY TO

  19. Regulatory effects of adenosine A2A receptors on psychomotor ability and mood behavior of mice%腺苷A2A受体对小鼠精神运动和情绪行为的调控作用

    Institute of Scientific and Technical Information of China (English)

    姜莉; 冉鸿; 段炜; 郑健

    2011-01-01

    目的 探讨腺苷A2A受体基因敲除、A2A受体激动剂干预或A2A受体抑制剂干预对小鼠运动活性、焦虑和抑郁样行为的影响.方法 取腺苷A2A受体基因敲除(A2AKO)的雄性小鼠(A2AKO组)及同窝野生型(WT)C57BL/6小鼠(WT组)待用.另取雄性清洁级C57BL/6小鼠,随机分为SCH58261组、CGS21680组和对照组,分别给予腺苷A2A受体特异性拮抗剂SCH58261(2mg/kg)、腺苷A2A受体特异性激动剂CGS21680(0.5mg/kg)和同体积(0.25ml)载体溶液(二甲基亚砜+生理盐水)腹膜腔注射,10min后待用.前述各组别小鼠均进行旷场、高架十字迷宫和强迫游泳实验,测定各组小鼠的运动活性、焦虑和抑郁样行为.结果 与WT组比较,A2AKO组旷场总运动路程缩短(P<0.001),周边区域的滞留时间延长(P<0.05),进入高架十字迷宫开臂的次数及在开臂滞留时间减少(P<0.05),强迫游泳的累计不动时间无显著差异(P>0.05),而CGS21680组旷场总运动路程缩短(P<0.01),周边区域滞留时间延长(P<0.01),进入高架十字迷宫开臂的次数及在开臂的滞留时间减少(P<0.01),强迫游泳的累计不动时间延长(P<0.001),而SCH58261组旷场总运动路程和中心区域活动路程延长(P<0.001),强迫游泳的累计不动时间缩短(P<0.01),高架十字迷宫试验各项指标无显著差异(P>0.05).结论 腺苷A2A受体激动剂可减少小鼠的自发和探索行为,加重焦虑和抑郁情绪,该效应与腺苷A2A受体基因敲除引发的效应相似,与腺苷A2A受体拮抗剂引发效应相反.%Objective To explore the effects of gene knock-out, agonist or inhibitor of adenosine A2A receptor on the locomotor activity, and anxiety- or depression-like behavior of mice. Methods Male C57BL/6 mice, comprising those underwent gene knock-out of adenosine AjA receptor (AjaKO) and their wild-type (WT) littermates, were assigned into A2A KO group and WT group. Another batch of male C57BL/6, specific

  20. Spomenik ninskom biskupu Francescu Grassiju u Chioggi: prilog najranijoj aktivnosti venecijanskog kipara Paola Callala

    Directory of Open Access Journals (Sweden)

    Damir Tulić

    2014-12-01

    Tagliapietre (Venecija, 1680. – 1747.. U Dalmaciji se relativno skroman opus dosada uočenih Callalovih sastoji od dva mramorna anđela postavljena uz glavni oltar u župnoj crkvi u Vodicama te četiri putta svirača smještena sa strane glavnog oltara, kao i onih na bočnom oltaru u župnoj crkvi u Sutivanu na otoku Braču. No, Callalovu ruku moguće je prepoznati i u jednoj skulpturi iz pozamašnog kiparskog programa na oltaru Presvetog Sakramenta u zadarskoj katedrali. Arhitekturu je 1719. izradio Antonio Viviani dok je bogatu kiparsku dekoraciju najvećim dijelom isklesao Francesco Cabianca (Venecija, 1666. - 1737.. U središnjoj niši oltara smješten je manji mramorni kip Žalosne Bogorodice s mrtvim Kristom u krilu. Mramorna zadarska Pietà teško se uklapa u katalog Francesca Cabiance, pogotovo ako se ima u vidu njegov kip istoimene tematike postavljen oko 1714. nad vrata u klaustru venecijanske crkve I Frari. Cabiancina venecijanska Pietà posjeduje oštrinu poteza dlijeta, određenu grubost izvedbe i robustnost tijela te drugačiju fizionomiju i draperiju od zadarskog djela. No, zadarska se Pietà može uključiti u katalog kipara Paola Callala. Pažljivo modelirani lik žalosne Majke te meko oblikovanu draperiju koja se oko stopala radijalno širi prema van možemo usporediti s kipovima Vjere i Nade na oltaru Sakramenta katedrale u Udine, nastalom nakon 1720. godine. Paralele u modeliranju Kristova tijela, a posebno lica suzdržane mimike, nalazimo na kipu Uskrslog na tabernakulu već spominjanog udineškog oltara, kao i u istovjetnom kipu na tabernakulu oltara župne crkve u Clauzettu, što ga također pripisujem Callalu, ali i na ranijim monumentalnijim primjerima, poput Krista s oltara Preobraženja nastalom 1708. za župnu crkvu u Labinu. Callalov spomenik ninskom biskupu Francescu Grassiju u Chioggi važan je pokazatelj razvoja osobnog stila majstora. Njegov se izričaj tako transformira iz ovog „mladenačkog djela“ punog siline i robusnosti u lirsku

  1. Book Reviews

    Directory of Open Access Journals (Sweden)

    Redactie KITLV

    1997-01-01

    NJ: Africa World Press, 1993. xxv + 115 pp. -Sandra Burr, Gretchen Gerzina, Black London: Life before emancipation. New Brunswick NJ: Rutgers University Press, 1995. xii + 244 pp. -Carlene J. Edie, Trevor Munroe, The cold war and the Jamaican Left 1950-1955: Reopening the files. Kingston: Kingston Publishers, 1992. xii + 242 pp. -Carlene J. Edie, David Panton, Jamaica's Michael Manley: The great transformation (1972-92. Kingston: Kingston Publishers, 1993. xx + 225 pp. -Percy C. Hintzen, Cary Fraser, Ambivalent anti-colonialism: The United States and the genesis of West Indian independence, 1940-1964. Westport CT: Greenwood, 1994. vii + 233 pp. -Anthony J. Payne, Carlene J. Edie, Democracy in the Caribbean: Myths and realities. Westport CT: Praeger, 1994. xvi + 296 pp. -Alma H. Young, Jean Grugel, Politics and development in the Caribbean basin: Central America and the Caribbean in the New World Order. Bloomington: Indiana University Press, 1995. xii + 270 pp. -Alma H. Young, Douglas G. Lockhart ,The development process in small island states. London: Routledge, 1993. xv + 275 pp., David Drakakis-Smith, John Schembri (eds -Virginia Heyer Young, José Solis, Public school reform in Puerto Rico: Sustaining colonial models of development. Westport CT: Greenwood Press, 1994. x + 171 pp. -Carolyn Cooper, Christian Habekost, Verbal Riddim: The politics and aesthetics of African-Caribbean Dub poetry. Amsterdam: Rodopi, 1993. vii + 262 pp. -Clarisse Zimra, Jaqueline Leiner, Aimé Césaire: Le terreau primordial. Tübingen: Gunter Narr Verlag, 1993. 175 pp. -Clarisse Zimra, Abiola Írélé, Aimé Césaire: Cahier d'un retour au pays natal. With introduction, commentary and notes. Abiola Írélé. Ibadan: New Horn Press, 1994. 158 pp. -Alvina Ruprecht, Stella Algoo-Baksh, Austin C. Clarke: A biography. Barbados: The Press - University of the West Indies; Toronto: ECW Press, 1994. 234 pp. -Sue N. Greene, Glyne A. Griffith, Deconstruction, imperialism and the West Indian novel

  2. Hepatocellular carcinoma. Comparison between gadolinium and ironoxide enhanced MR imaging; Risonanza Magnetica epatica ed epatocarcinoma. Confronto fra risultati ottenuti con mezzo di contrasto paramagnetico (gadolinio) e superparamagnetico (particelle di ossido di ferro)

    Energy Technology Data Exchange (ETDEWEB)

    Castoldi, M.C.; Fauda, V.; Scaramuzza, D.; Vergnaghi, D. [Ospedale S. Carlo Borromeo, Milan (Italy). Servizio di Radiologia

    2000-09-01

    soli 3 casi le sequenze TSE T2 pesate hanno avuto rilevanza diagnostica rispetto a quelle GE. Con tecnica TSE sono risultate pero' ben evidenti alcune caratteristiche morfologiche dei noduli (presenza di capsula, di setti, di componenti a diversa differenziazione cellulare), non riconoscibili nelle immagini GE. L'associazione di sequenze TSE T2 pesate e GE T1-T2* pesate dopo mdc ferroso, si e' rivelata piu' sensibile della RM dinamica con gadolinio condotta con sequenza GE (percentuale di identificazione rispettivamente del 97,7 e 75%). I risultati ottenuti, favorevoli all'impiego di mdc ferrosi nell'epatocarcinoma, sono almeno in parte da mettere in relazione alle nostre condizioni di studio e cioe': 1) alla disponibilita' di campo magnetico da 1,0 T, 2) al tipo di esame di RM eseguito dopo mdc ferrosi (utilizzazione di sequenze GE con pesatura T1-T2* e di immagini assiali e sagittali), 3) alla scarsa rappresentazione percentuale di cirrosi di grado elevato nella casistica.

  3. Indicadores de qualidade de solos de área de mineração e metalurgia de chumbo: II - Mesofauna e plantas Soil quality indicators in lead mining and metalurgy area: II - Mesofauna and plants

    Directory of Open Access Journals (Sweden)

    Yara Jurema Barros

    2010-08-01

    riqueza de grupos de organismos e ocorrência de representantes dos grupos Pseudoscorpiones, Mollusca e Isopoda apenas nesse solo. Os grupos Aracnídeos e Psocoptera também foram considerados bons indicadores ambientais, com incremento de suas populações nos solos com maiores teores de metais pesados (solos 2, 3 e 5, possivelmente devido à menor ocorrência de organismos competidores/predadores desses grupos. Os teores de metais pesados nos indivíduos do grupo Formicidae tiveram relação direta com os teores de Pb extraídos com HNO3 0,5 mol L-1 no solo. Quanto ao acúmulo de metais pesados nas plantas coletadas na área, com exceção do solo 1, todas as espécies encontravam-se sob efeito fitotóxico para Pb e Zn, o que sugere a proibição de pastejo na área.The influence of soil management or the contaminant application to the soil, usually induce to a quicker response in the soil mesofauna than in other pedogenic properties; these organisms are therefore good environmental quality indicators. The aim of this study was to identify and quantify the organisms groups of the soil mesofauna and determine Pb and Zn contents in plants in the mining and metallurgy plant in Adrianópolis (PR,to originate biological indicators of the quality of these solis. At the selected locations the following contamination forms were analized: site 1 - reference (native wood; site 2 - residue incorporated in the profile; sites 3 and 6 - close to the chimneys of the plant, with potential import of particulate matter; site 5 - great waste volume covering the soil. Berlese funnels were utilized to collect samples from the 0 to 5 cm layer (20 funnels x 5 sites x 1 layer x 4 times = 400 samples. After the separation of the mesofauna, the organisms were selected and identified. Ants samples were microwave digested with concentrated HNO3 and the Pb, Cu, Ni and Zn contents were determined by inductively coupled plasma - atomic emission Spectrometry (ICP-AES. Plants from Poaceae family were

  4. Research on the Nature of Thermophilic Anaerobic Ethanol Producer Thermo anaerobacter sp DF3 in Petroleum Reservoirs%一株产乙醇嗜热厌氧油藏微生物ThermoanaerobacterspDF3的性质研究

    Institute of Scientific and Technical Information of China (English)

    兰贵红; 邢钰; 曹毅; 乔代蓉; 邹长军; 邓宇; 张辉; 尹小波

    2012-01-01

    [目的]了解油藏微生物ThermoanaerobacterspDF3的生理生化特性,优化木糖产乙醇培养方案。[方法]利用厌氧分离技术从大港油田油层采出液中分离到一株产乙醇厌氧杆菌DF3采用生理生化鉴定与16SrDNA序列的系统发育学分析其系统发育地位,用气相色谱分析其代谢产物。[结果]菌株DF3是一株严格厌氧的嗜热细菌,呈直杆状,G-菌体大小为O.42μm×(1.60~5.20)μm,单生成对或成串生,产顶端芽孢;生长温度为45~78℃(最适65℃),能利用葡萄糖、木糖、果糖、核糖、甘露糖、阿拉伯糖、蔗糖、半乳糖、乳糖、纤维二糖、松三糖、棉子糖、淀粉等作为底物;其16SrRNA与zpseud。ethanolicw相似性为99.7%发酵葡萄糖与木糖的主要产物为乙醇,培养方案优化后其代谢木糖产乙醇终浓度为2.0g/L。[结论]通过试验证明菌株DF3是目前已知菌株中产乙醇活性较强的菌株之一,在65℃时代谢木糖能产生2.0g/L的乙醇目前代谢木糖高产乙醇的菌株均由国外分离获得,菌株DF3的分离获得为我国研究木质纤维素产乙醇提供了优良的出发菌株。%[Objective] The aim was to study the physiological and biochemical char- acteristics of Thermoanaerobacter sp DF3 in petroleum reservoirs and optimize the culture plan of producing ethanol from xylose. [Method] DF3, an anaerobic bacillus producing ethanol, was isolated from produced liquid from oil layer of Dagang oil field with anaerobic isolation technique. The phylogenetic position was analyzed by physiological and biochemical identification and phylogeny of 16S rDNA sequence. The metabolites were analyzed by gas chromatograph. [Result] The strain DF3 was a strict anaerobic thermophilic bacterium, which was straight in rod shape,and gram negative. Besides, it was 0.42 μmx(1.60-5.20) iJm in length. The strains can be soli- tary,in pairs or string and apical spore usually

  5. Improving oxidative stress response and reversing abnormal expression of hippocampus NET and 5-HTT involved in anti-depressive effect of venlafaxine%改善氧化应激平衡和逆转NET及5-HTT表达异常涉及文拉法辛的抗抑郁作用

    Institute of Scientific and Technical Information of China (English)

    胡小娅; 费慧芝; 蒋心惠; 刘丹; 邱红梅; 周岐新

    2014-01-01

    Aim To investigate if anti-depressive effect of venlafaxine was associated with improving oxi-dative stress and expression of hippocampus NET and 5-HTT in rat model induced by CUS. MethodsEighty SD male rats were randomly divided into four groups:model group(MG),normal group(NG), ven-lafaxine-treated normal group ( VNG ) , and venlafax-ine-treated model group ( VMG) . VNG and VMG were given venlafaxine (23. 4 mg·kg-1 ·d-1 ) once daily;NG and MG were given the same volume solvent. Soli-tary condition with chronic unpredicted stress ( CUS ) was taken to establish rat depression model. The force swimming test was used to evaluate the behavior chan-ges of experimental rats. The malondialdehyde ( MDA) level and activity of superoxide dismutase ( SOD ) in serum were determined by biochemical methods. The mRNA and protein expressions of NET and 5-HTT in hippocampus were determined by Real-Time Reverse transcription polymerase chain reaction ( real-time RT-PCR) and Western blot ( WB) , respectively. Results Compared with NG rats, obviously increasing immo-bile time of rats in force swimming test and serum MDA level, as well as significantly decreasing SOD activity in serum was observed with clearly decreasing 5-HTT expression and elevating NET expression in hippocam-pus of MG rats. The treatment of venlafaxine distinctly suppressed changes above from CUS-induced rats. However, significant changes failed to be found in VNG rats. Conclusion The anti-depressive effect of venlafaxine may at least partly involve in improving ox-idative stress/anti-oxidative stress balance and revers-ing abnormal expression of NET and 5-HTT.%目的:探讨文拉法辛抗慢性不可预见刺激所致大鼠抑郁症作用与氧化应激和海马去甲肾上腺素转运体( norepi-nephrine transporter,NET)、5羟色胺转运体(serotonin trans-porter,5-HTT)表达的关系。方法裔SD大鼠80只,根据开场实验得分分为4组,即正常对照组( NG)、模型组( MG)、文拉

  6. 改善氧化应激平衡和逆转NET及5-HTT表达异常涉及文拉法辛的抗抑郁作用%Improving oxidative stress response and reversing abnormal expression of hippocampus NET and 5-HTT involved in anti-depressive effect of venlafaxine

    Institute of Scientific and Technical Information of China (English)

    胡小娅; 费慧芝; 蒋心惠; 刘丹; 邱红梅; 周岐新

    2014-01-01

    目的:探讨文拉法辛抗慢性不可预见刺激所致大鼠抑郁症作用与氧化应激和海马去甲肾上腺素转运体( norepi-nephrine transporter,NET)、5羟色胺转运体(serotonin trans-porter,5-HTT)表达的关系。方法裔SD大鼠80只,根据开场实验得分分为4组,即正常对照组( NG)、模型组( MG)、文拉法辛处理的正常对照组( VNG)和文拉法辛处理的模型组( VMG)。采用孤养结合慢性不可预见刺激( chronic unpre-dictable stress,CUS)方法建立大鼠抑郁模型;给药组每天给予文拉法辛(23.4 mg·kg-1·d-1),NG和MG组每天给予同体积溶剂。采用强迫游泳( force swimming)实验评价大鼠的抑郁行为,生物化学方法检测大鼠血清的超氧化物岐化酶( superoxide dismutase,SOD)活性与丙二醛( malondialdehyde, MDA)含量,实时定量RT-PCR分析海马5-HTT、NET的mR-NA表达, Western blot 测定海马5-HTT、NET 的蛋白表达。结果相对于NG组,MG组大鼠在强迫游泳实验中的不动时间明显增加,SOD活性降低,血清MDA含量升高,海马5-HTT表达明显下降,而NET的表达明显升高。文拉法辛干预能阻遏CUMS诱导的上述变化;文拉法辛处理对正常对照大鼠的行为、SOD、MDA和5-HTT及NET表达无明显影响。结论文拉法辛抑制CUS诱导大鼠出现焦虑抑郁样行为的机制至少部分与其改善脑内氧化应激平衡,逆转5-HTT和NET异常表达有关。%Aim To investigate if anti-depressive effect of venlafaxine was associated with improving oxi-dative stress and expression of hippocampus NET and 5-HTT in rat model induced by CUS. MethodsEighty SD male rats were randomly divided into four groups:model group(MG),normal group(NG), ven-lafaxine-treated normal group ( VNG ) , and venlafax-ine-treated model group ( VMG) . VNG and VMG were given venlafaxine (23. 4 mg·kg-1 ·d-1 ) once daily;NG and MG were given the same volume solvent. Soli-tary condition with chronic unpredicted stress ( CUS ) was taken to

  7. Metod vektora sličnosti idealu za višeatributno odlučivanje / Method of the vector of similarity to ideal solution in alternatives / Метод вектора сходства вариантов с идеальным решением

    Directory of Open Access Journals (Sweden)

    Radomir R. Đukić

    2016-01-01

    Full Text Available U članku se razmatra postupak rešavanja problema višeatributnog odlučivanja na jednom nivou kriterijuma i prezentuje metod zasnovan na elementima kompromisnog odlučivanja, Lp metrici i TOPSIS metodu. Daju se preporuke za formiranje početne matrice odlučivanja i transformaciju raznorodnih kriterijumskih vrednosti. Kompromisna rešenja dobijaju se na osnovu vrednosti funkcija Lp metrike i njihovih kombinacija sa koeficijentima − funkcijama relativne verodostojnosti zavisnim od dimenzija problema. Dobijena rešenja zavisna su od parametra p u Lp metrici koji je balansirajući faktor između rešenja sa najvećom ukupnom koristi i rešenja sa minimaks odstupanjima kriterijumskih vrednosti od ideala i antiideala. Ako se zahteva jedinstveno rešenje, ono se dobija objedinjavanjem svih funkcija Lp metrike i primenom vektora sličnosti varijanata idealu, kojim je obuhvaćen i uticaj antiideala. Prikazan je i postupak dobijanja kompromisnih rešenja na osnovu vrednosti elemenata parcijalnih vektora sličnosti idealu i uticaj subjektivno određenog antiideala na rešenja. Primena metoda prikazana je numeričkim primerom. / A method for solving multiple-attribute decision-making problems at one criterion level is discussed, followed by a presentation of a method based on compromise programming elements, Lp metrics and the TOPSIS method. Suggestions for forming the initial decisionmaking matrix have been given as well as for the transformation of multiple criteria values. Compromise solutions are obtained based on the values of Lp metrics functions and their combinations with coefficients – functions of relative credibility depending on solution dimensions. The obtained solutions depend on the parameter p in Lp metrics, parameter p being a balancing factor between the solution with the highest total utility and the solution with minimax deviations of criteria values from the ideal and negative-ideal solutions. If a unique solution is required, it

  8. The Application of Coreless Inductors for Displacement Measurements in Laboratory Investigations of Rock Properties

    Science.gov (United States)

    Nurkowski, Janusz

    2014-12-01

    ąt procent. Przykładowe pomiary przedstawiają rysunki 8 i 9. Na rys. 10 pokazano wyniki testu pomiaru ściśliwości stali, przy użyciu czujnika referencyjnego. W trzech cyklach obciążania, podczas których zmiany temperatury wywołane sprężaniem i rozprężaniem cieczy (do 350 MPa) sięgały kilkunastu °C. Histereza i rozrzut pomiaru w kolejnych cyklach wynosiły najwyżej kilka mikrometrów przy rozdzielczości około 0.2 μm. Czujnik stosowany jest również w pomiarach poza komorą ciśnieniową. Np. fotografia (rys. 11) przedstawia czujnik przy pomiarze ugięcia próbki drewna pobranego w kopalni soli Wieliczka. Fotografia na rys. 13 przedstawia stanowisko do pomiaru deformacji osiowych i obwodowych brykietu węglowego podczas testu jednoosiowego ściskania. Drugi typ czujnika, do pomiaru dużych odkształceń obwodowych (kilkadziesiąt procent) w teście konwencjonalnego trójosiowego ściskania, w którym próbka jest jednocześnie ściskana ciśnieniem hydrostatycznym (okólnym) a następnie obciążana osiowo tłokiem prasy poruszającym się wewnątrz komory ciśnieniowej. W ciśnieniu hydrostatycznym setek MPa na ogół skały zachowują się plastycznie i w teście tym siła działająca osiowo na cylindryczną próbkę powoduje odkształcenie jej nawet o kilkadziesiąt procent, do postaci beczki. Pomiar odkształceń obwodowych jest realizowany czujnikiem indukcyjnym uformowanym na kształt torusa, przez spięcie jego końców izolacyjną płytką (rys. 1). Czujnik na próbce utrzymywany jest dzięki sile sprężystości jego zwojów. Na rys. 14. pokazano efekty trójosiowego testu: odkształcenie osiowe ɛ1 (pomiar ruchu tłoka prasy, na zewnątrz komory) i poprzeczne ɛ3 (czujnikiem toroidalnym) oraz zmianę objętości ΔV, walcowej próbki dolomitu. Jeśli nie są mierzone deformacje poprzeczne, to aktualny przekrój próbki wyliczany jest na podstawie odkształcenia osiowego, przy założeniu stałości objętości próbki (ν = const. = 0

  9. Prikazi

    Directory of Open Access Journals (Sweden)

    Drahomira Gavranović

    2012-02-01

    zbornika jest i tema vlasništva pojedinog primjerka stare knjige. Upravo tu temu obradio je D. Shaw pod naslovom Tko je bio vlasnik ove knjige? Proučavanje porijekla knjiga u europskim razmjerima. Autor naglašava važnost katalogizacije na temelju pojedinog primjerka jer se jedino tako u kataložnom zapisu mogu zabilježiti sve karakteristike i posebnosti pojedinog primjerka građe. Upravo takav postupak opisa potaknuo je razvoj područja kojim se bavi autor u svome radu - proučavanja porijekla knjige. Važnost proučavanja prethodnih vlasnika jasno se vidi u mogućnosti okupljanja raspršenih knjižnica i privatnih zbirki koje svoju „pripadnost“ određenoj zbirci ponekad mogu dokazati jedino obilježjima vlasnika ili knjižnice kojoj su nekoć pripadali. Osim vlastoručnih potpisa vlasnika i pečata knjižnice, dokazi o prijašnjim vlasnicima mogu se istražiti i uz pomoć signatura, ex librisa, pečata na uvezima itd. Veliku pomoć u toj vrsti istraživanja pruža i Konzorcij europskih knjižnica (CERL koji nudi mrežnu bazu podataka o porijeklu knjiga. Posljednji rad u ovoj cjelini izradila je doktorska studentica M. Tomić pod naslovom Podaci o povijesti primjerka u hrvatskim katalozima stare i rijetke građe. Autorica je prikupila dostupne (tiskane kataloge te je kroz te kataloge i kataložne zapise starih i rijetkih knjiga u online katalozima istražila koliko se bilježe podaci o porijeklu primjerka. U radu su prikazani rezultati spomenutog istraživanja s posebnim osvrtom na podatke o povijesti primjerka ukoliko su (i ako su uopće uključeni u tiskane kataloge stare i rijetke građe u hrvatskim knjižnicama te u online katalozima hrvatskih knjižnica. Dodana vrijednost ovoga rada jest i bibliografija kataloga inkunabula, kataloga knjiga i rukopisa koji se nalaze u muzejskim knjižnicama i crkvenim ustanovama te kataloga izložbi starih i rijetkih knjiga. Neki od izvora koji su istraženi nisu ranije uključivani u postojeće bibliografije stare gra