WorldWideScience

Sample records for anorganickych soli ako

  1. SOLIS/VSM Polar Magnetic Field Data

    CERN Document Server

    Bertello, Luca

    2015-01-01

    The Vector Spectromagnetograph (VSM) instrument on the Synoptic Optical Long-term Investigations of the Sun (SOLIS) telescope is designed to obtain high-quality magnetic field observations in both the photosphere and chromosphere by measuring the Zeeman-induced polarization of spectral lines. With 1$^{\\prime \\prime}$ spatial resolution (1.14$^{\\prime \\prime}$ before 2010) and 0.05\\AA\\ spectral resolution, the VSM provides, among other products, chromospheric full-disk magnetograms using the CaII 854.2 nm spectral line and both photospheric full-disk vector and longitudinal magnetograms using the FeI 630.15 nm line. Here we describe the procedure used to compute daily weighted averages of the photospheric radial polar magnetic field at different latitude bands from SOLIS/VSM longitudinal full-disk observations. Time series of these measurements are publicly available from the SOLIS website at http://solis.nso.edu/0/vsm/vsm\\_plrfield.html. Future plans include the calculation of the mean polar field strength fr...

  2. 7. juunil kaitses Tallinna Ülikoolis oma doktoritööd Ako Sauga

    Index Scriptorium Estoniae

    2006-01-01

    Ako Sauga doktoritöö teema oli "Influence of environmental fluctuations on dynamics of non-linear systems" (pealkiri eesti keeles : "Keskkonnaparameetrite fluktuatsioonide mõju mittelineaarsete süsteemide dünaamikale")

  3. A Far-Ultraviolet Survey of 47 Tucanae.II The Long-Period Cataclysmic Variable AKO 9

    CERN Document Server

    Knigge, C; Shara, M M; Long, K S; Gilliland, R L; Knigge, Christian; Zurek, David. R.; Shara, Michael M.; Long, Knox S.; Gilliland, Ronald L.

    2003-01-01

    We present time-resolved, far-ultraviolet (FUV) spectroscopy and photometry of the 1.1 day eclipsing binary system AKO 9 in the globular cluster 47 Tucanae. AKO 9's FUV spectrum is blue and exhibits prominent C IV and He II emission lines. The spectrum broadly resembles that of long-period, cataclysmic variables in the galactic field. Combining our time-resolved FUV data with archival optical photometry of 47 Tuc, we refine AKO 9's orbital period and define an accurate ephemeris for the system. We also place constraints on several other system parameters, using a variety of observational constraints. We find that all of the empirical evidence is consistent with AKO 9 being a long-period dwarf nova in which mass transfer is driven by the nuclear expansion of a sub-giant donor star. We therefore conclude that AKO 9 is the first spectroscopically confirmed cataclysmic variable in 47 Tuc. We also briefly consider AKO 9's likely formation and ultimate evolution. Regarding the former, we find that the system was al...

  4. Inversions of High-Cadence SOLIS-VSM Stokes Observations

    NARCIS (Netherlands)

    Fischer, C.E.; Keller, C.U.; Snik, F.

    2008-01-01

    We have processed full-Stokes observations made with the SOLIS-VSM using Fe I 630.15 and Fe I 630.25 nm. The data have high spectral and temporal resolution, moderate spatial resolution, and large polarimetric sensitivity and accuracy. We use the code LILIA, an LTE inversion code written by Socas-Na

  5. A functional pectin methylesterase inhibitor protein (SolyPMEI) is expressed during tomato fruit ripening and interacts with PME-1.

    Science.gov (United States)

    Reca, Ida Barbara; Lionetti, Vincenzo; Camardella, Laura; D'Avino, Rossana; Giardina, Thierry; Cervone, Felice; Bellincampi, Daniela

    2012-07-01

    A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects the activity of plant pectin methylesterases (PMEs) and is inactive against a microbial PME. SolyPMEI transcripts were mainly expressed in flower, pollen and ripe fruit where the protein accumulated at breaker and turning stages of ripening. The expression of SolyPMEI correlated during ripening with that of PME-1, the major fruit specific PME isoform. The interaction of SolyPMEI with PME-1 was demonstrated in ripe fruit by gel filtration and by immunoaffinity chromatography. The analysis of the zonal distribution of PME activity and the co-localization of SolyPMEI with high esterified pectins suggest that SolyPMEI regulates the spatial patterning of distribution of esterified pectins in fruit.

  6. Initial Operation and Calibration of the SOLIS Vector Spectromagnetograph

    Science.gov (United States)

    Jones, H. P.; Harvey, J. W.; Henney, C. J.; Keller, C. U.

    2003-01-01

    The National Solar Observatory's SOLIS Vector Spectromagnetograph (VSM) is designed to observe the Sun's vector magnetic field over the entire visible disk several times per day for at least two decades and will provide important new information about the drivers of CMEs and other s o l a r activity affecting the heliosphere. The VSM is being prepared for temporary installation at the agricultural site of the University of Arizona. We report on the status of the instrument, emphasizing early data and their comparison with observations from the NASA/NSO Spectromagnetograph at the Kitt Peak Vacuum Telescope and the HAO/NSO Advanced Stokes Polarimeter at the Sacramento Peak Dunn Vacuum Tower Telescope.

  7. User experience network. Erroneous downstream occlusion alarms may disable Smiths Medical CADD-Solis infusion pumps.

    Science.gov (United States)

    2010-10-01

    Due to an issue in manufacturing, downstream occlusion (DSO) sensors in some Smiths Medical CADD-Solis infusion pumps may drift out of calibration, potentially resulting in erroneous alarms that disable the units. Hospitals experiencing the problem should return affected units to Smiths Medical for recalibration (free of charge) and should consider testing all their CADD-Solis pumps during routine maintenance to ensure that they alarm appropriately for downstream occlusions.

  8. Current Status of the SOLIS Program: Improved and New Data Products

    Science.gov (United States)

    Bertello, Luca; Britanik, John; Callahan, Lorraine; Gosain, Sanjay; Harker, Brian; Harvey, J. W.; Hughes, A.; Marble, A.; Pevtsov, Alexei A.; Wentzel, Thomas

    2016-05-01

    Over the past year the Synoptic Optical Long-term Investigations of the Sun (SOLIS) team has made significant improvements to the data products provided to the solar and heliospheric community. In particular, a considerable effort has been dedicated to reprocess the archive of vector and longitudinal photospheric magnetograms, from 2003 to present, using the latest production code. This endeavor is now near completion and will assure that all derived magnetic products, such as synoptic maps and flux time series, are consistently calibrated. In addition, new products have been recently developed. For example, time series of daily averages of the Sun's polar magnetic field derived from full-disk photospheric FeI 630.15 nm longitudinal magnetograms are now available from the SOLIS website at solis.nso.edu/0/vsm/vsm_plrfield.html.New intensity calibration of high resolution Ca II K & H spectra from the Integrated Sunlight Spectrometer (ISS) led to significant reduction in daily variations of parameters derived from these spectra. The SOLIS team will soon also release Carrington synoptic maps for the three components of the photospheric magnetic field derived from vector measurements taken in the Fe I 630.15 nm spectral line. The addition, in late 2015, of a new Ca II 854.2 nm full-Stokes polarimeter into the SOLIS core program of magnetic observations will make available to the community daily chromospheric measurements of the complete Stokes polarization vector.We present here a summary of these improvements, with particular emphasis on the new products that can be accessed from the SOLIS data page at solis.nso.edu/0/solis_data.html. For a description of the diagnostic capability of the new Ca II 854.2 nm spectro-polarimeter and preliminary results we refer to other presentations by SOLIS team members at this meeting.This work utilizes SOLIS data obtained by the NSO Integrated Synoptic Program (NISP), managed by the National Solar Observatory, which is operated by the

  9. Pseudomonas soli sp. nov., a novel producer of xantholysin congeners.

    Science.gov (United States)

    Pascual, Javier; García-López, Marina; Carmona, Cristina; Sousa, Thiciana da S; de Pedro, Nuria; Cautain, Bastien; Martín, Jesús; Vicente, Francisca; Reyes, Fernando; Bills, Gerald F; Genilloud, Olga

    2014-09-01

    A chemoorganotrophic Gram-negative bacterium was isolated by means of a diffusion sandwich system from a soil sample from the Sierra Nevada National Park, Spain. Strain F-279,208(T) was oxidase and catalase positive, strictly aerobic, non-spore-forming and motile by single polar flagellum. Phylogenetic analysis of the 16S rRNA, gyrB, rpoB and rpoD genes revealed that strain F-279,208(T) belongs to the Pseudomonas putida group with Pseudomonas mosselii and Pseudomonas entomophila as its closest relatives. DNA-DNA hybridization assays and phenotypic traits confirmed that this strain belongs to a novel species of the genus Pseudomonas, for which the name Pseudomonas soli sp. nov. is proposed. The type strain is F-279,208(T) (=DSM 28043(T)=LMG 27941(T)), and during fermentation it produces xantholysins, a family of lipodepsipeptides. The major compound, xantholysin A, showed an interesting activity in a RCC4 kidney tumor cell line with inactivation of VHL linked with the HIF pathway, without any cytotoxic effects against other human tumor cell lines tested including, liver, pancreas and breast.

  10. Retrato de estudio. Hombre con barba, bigote y gafas. Gumersindo Solis. Barcelona.

    OpenAIRE

    García, E.

    2010-01-01

    1 fot.; papel; imagen 25 x 30,9 cm. - Retrato de estudio. Hombre con barba, bigote y gafas. Gumersindo Solis. Barcelona. (Gelatina D. O. P. mate montada sobre cartulina, medida total 25 x 30,9. Imagen densa. Cartulina sucia. Sello troquelado del fotógrafo en margen inferior de cartulina: "E. García, Paseo del Borne, Sucursal Napoleón - Barcelona" y nombre manuscrito a tinta negra en reverso: "Gumersindo Solis"). - Procedencia: fondo Miguel de Unamuno. - Buena Conservación.

  11. High frequency of Acinetobacter soli among Acinetobacter isolates causing bacteremia at a tertiary hospital in Japan.

    Science.gov (United States)

    Endo, Shiro; Yano, Hisakazu; Kanamori, Hajime; Inomata, Shinya; Aoyagi, Tetsuji; Hatta, Masumitsu; Gu, Yoshiaki; Tokuda, Koichi; Kitagawa, Miho; Kaku, Mitsuo

    2014-03-01

    Acinetobacter baumannii is generally the most frequently isolated Acinetobacter species. Sequence analysis techniques allow reliable identification of Acinetobacter isolates at the species level. Forty-eight clinical isolates of Acinetobacter spp. were obtained from blood cultures at Tohoku University Hospital. These isolates were identified at the species level by partial sequencing of the RNA polymerase β-subunit (rpoB), 16S rRNA, and gyrB genes. Then further characterization was done by using the PCR for detection of OXA-type β-lactamase gene clusters, metallo-β-lactamases, and carO genes. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing were also performed. The most frequent isolate was Acinetobacter soli (27.1%). Six of the 13 A. soli isolates were carbapenem nonsusceptible, and all of these isolates produced IMP-1. PFGE revealed that the 13 A. soli isolates were divided into 8 clusters. This study demonstrated that A. soli accounted for a high proportion of Acinetobacter isolates causing bacteremia at a Japanese tertiary hospital. Non-A. baumannii species were identified more frequently than A. baumannii and carbapenem-nonsusceptible isolates were found among the non-A. baumannii strains. These results emphasize the importance of performing epidemiological investigations of Acinetobacter species.

  12. Biotransformation of ferulic acid to 4-vinylguaiacol by Enterobacter soli and E. aerogenes.

    Science.gov (United States)

    Hunter, William J; Manter, Daniel K; van der Lelie, Daniel

    2012-12-01

    We investigated the conversion of ferulic acid to 4-vinylguaiacol (4-VG), vanillin, vanillyl alcohol, and vanillic acid by five Enterobacter strains. These high-value chemicals are usually synthesized by chemical methods but biological synthesis adds market value. Ferulic acid, a relatively inexpensive component of agricultural crops, is plentiful in corn hulls, cereal bran, and sugar-beet pulp. Two Enterobacter strains, E. soli, and E. aerogenes, accumulated 550-600 ppm amounts of 4-VG when grown in media containing 1,000 ppm ferulic acid; no accumulations were observed with the other strains. Decreasing the amount of ferulic acid present in the media increased the conversion efficiency. When ferulic acid was supplied in 500, 250, or 125 ppm amounts E. aerogenes converted ~72 % of the ferulic acid present to 4-VG while E. soli converted ~100 % of the ferulic acid to 4-VG when supplied with 250 or 125 ppm amounts of ferulic acid. Also, lowering the pH improved the conversion efficiency. At pH 5.0 E. aerogenes converted ~84 % and E. soli converted ~100 % of 1,000 ppm ferulic acid to 4-VG. Only small, 1-5 ppm, accumulations of vanillin, vanillyl alcohol, and vanillic acid were observed. E. soli has a putative phenolic acid decarboxylase (PAD) that is 168 amino acids long and is similar to PADs in other enterobacteriales; this protein is likely involved in the bioconversion of ferulic acid to 4-VG. E. soli or E. aerogenes might be useful as a means of biotransforming ferulic acid to 4-VG.

  13. Hybrid De Novo Genome Assembly Using MiSeq and SOLiD Short Read Data.

    Science.gov (United States)

    Ikegami, Tsutomu; Inatsugi, Toyohiro; Kojima, Isao; Umemura, Myco; Hagiwara, Hiroko; Machida, Masayuki; Asai, Kiyoshi

    2015-01-01

    A hybrid de novo assembly pipeline was constructed to utilize both MiSeq and SOLiD short read data in combination in the assembly. The short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components such as ABySS and SOAPdenovo. The assembly pipeline proceeded through several stages, and either MiSeq paired-end data, SOLiD mate-paired data, or both of them could be specified as input data at each stage separately. The pipeline was examined on the filamentous fungus Aspergillus oryzae RIB40, by aligning the assembly results against the reference sequences. Using both the MiSeq and the SOLiD data in the hybrid assembly, the alignment length was improved by a factor of 3 to 8, compared with the assemblies using either one of the data types. The number of the reproduced gene cluster regions encoding secondary metabolite biosyntheses (SMB) was also improved by the hybrid assemblies. These results imply that the MiSeq data with long read length are essential to construct accurate nucleotide sequences, while the SOLiD mate-paired reads with long insertion length enhance long-range arrangements of the sequences. The pipeline was also tested on the actinomycete Streptomyces avermitilis MA-4680, whose gene is known to have high-GC content. Although the quality of the SOLiD reads was too low to perform any meaningful assemblies by themselves, the alignment length to the reference was improved by a factor of 2, compared with the assembly using only the MiSeq data.

  14. Hybrid De Novo Genome Assembly Using MiSeq and SOLiD Short Read Data.

    Directory of Open Access Journals (Sweden)

    Tsutomu Ikegami

    Full Text Available A hybrid de novo assembly pipeline was constructed to utilize both MiSeq and SOLiD short read data in combination in the assembly. The short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components such as ABySS and SOAPdenovo. The assembly pipeline proceeded through several stages, and either MiSeq paired-end data, SOLiD mate-paired data, or both of them could be specified as input data at each stage separately. The pipeline was examined on the filamentous fungus Aspergillus oryzae RIB40, by aligning the assembly results against the reference sequences. Using both the MiSeq and the SOLiD data in the hybrid assembly, the alignment length was improved by a factor of 3 to 8, compared with the assemblies using either one of the data types. The number of the reproduced gene cluster regions encoding secondary metabolite biosyntheses (SMB was also improved by the hybrid assemblies. These results imply that the MiSeq data with long read length are essential to construct accurate nucleotide sequences, while the SOLiD mate-paired reads with long insertion length enhance long-range arrangements of the sequences. The pipeline was also tested on the actinomycete Streptomyces avermitilis MA-4680, whose gene is known to have high-GC content. Although the quality of the SOLiD reads was too low to perform any meaningful assemblies by themselves, the alignment length to the reference was improved by a factor of 2, compared with the assembly using only the MiSeq data.

  15. 美国第一位头颈外科医师Jacob Da Silva Solis-Cohen

    Institute of Scientific and Technical Information of China (English)

    王荣光

    2010-01-01

    @@ Jacob Da Silva Solis.Cohen(1838~1927)是19世纪末20世纪初杰出的内科和外科医师,他的从医生涯记录了喉科学和头颈外科学的起源与发展. 1838年2月29日Jacob Da Silva Solis-Cohen出生于美国纽约,他的先辈在犹太区非常知名,在北美革命期间是著名的慈善家和爱国者,1840年Solis-Cohen全家移居费城.Solis-Cohen在费城完成了大学教育,随后在Jefferson医学院学习,1860年他在Pennsylvania大学完成医学教育后在费城医院担任了6个月的住院医师.

  16. PROTIKOROZIJSKA ZAŠČITA ALUMINIJEVE ZLITINE V KLORIDNEM MEDIJU Z INHIBITORIJI NA OSNOVI LANTANOIDNIH SOLI

    OpenAIRE

    Volarič, Barbara

    2013-01-01

    Inhibicija korozijskih procesov na aluminijevih zlitinah z lantanoidnimi solmi bi lahko bila potencialno nadomestilo škodljivim kancerogenim kromatnim Cr6+ prevlekam, saj so lantanoidne soli neškodljive za okolje, so cenovno ugodne in jih lahko v večjih količinah najdemo v okolju. Aluminijevo zlitino 7075-T6 smo protikorozijsko zaščitili s korozijskimi inhibitorji, lantanoidnimi solmi cerija, lantana in neodima. Raziskovalno delo je bilo razdeljeno na dve področji: a) raziskovanje dodatka ...

  17. Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE) Prostate Cancer Tissue

    Science.gov (United States)

    Menon, Roopika; Deng, Mario; Boehm, Diana; Braun, Martin; Fend, Falko; Boehm, Detlef; Biskup, Saskia; Perner, Sven

    2012-01-01

    Next generation sequencing (NGS) technologies have revolutionized cancer research allowing the comprehensive study of cancer using high throughput deep sequencing methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation and Detection, Life Technologies) is a promising technology generating billions of 50 bp sequencing reads. This robust technique, successfully applied in gene identification, might be helpful in detecting novel genes associated with cancer initiation and progression using formalin fixed paraffin embedded (FFPE) tissue. This study’s aim was to compare the validity of whole exome sequencing of fresh-frozen vs. FFPE tumor tissue by normalization to normal prostatic FFPE tissue, obtained from the same patient. One primary fresh-frozen sample, corresponding FFPE prostate cancer sample and matched adjacent normal prostatic tissue was subjected to exome sequencing. The sequenced reads were mapped and compared. Our study was the first to show comparable exome sequencing results between FFPE and corresponding fresh-frozen cancer tissues using SOLiD sequencing. A prior study has been conducted comparing the validity of sequencing of FFPE vs. fresh frozen samples using other NGS platforms. Our validation further proves that FFPE material is a reliable source of material for whole exome sequencing. PMID:22942743

  18. Exome Enrichment and SOLiD Sequencing of Formalin Fixed Paraffin Embedded (FFPE Prostate Cancer Tissue

    Directory of Open Access Journals (Sweden)

    Saskia Biskup

    2012-07-01

    Full Text Available Next generation sequencing (NGS technologies have revolutionized cancer research allowing the comprehensive study of cancer using high throughput deep sequencing methodologies. These methods detect genomic alterations, nucleotide substitutions, insertions, deletions and copy number alterations. SOLiD (Sequencing by Oligonucleotide Ligation and Detection, Life Technologies is a promising technology generating billions of 50 bp sequencing reads. This robust technique, successfully applied in gene identification, might be helpful in detecting novel genes associated with cancer initiation and progression using formalin fixed paraffin embedded (FFPE tissue. This study’s aim was to compare the validity of whole exome sequencing of fresh-frozen vs. FFPE tumor tissue by normalization to normal prostatic FFPE tissue, obtained from the same patient. One primary fresh-frozen sample, corresponding FFPE prostate cancer sample and matched adjacent normal prostatic tissue was subjected to exome sequencing. The sequenced reads were mapped and compared. Our study was the first to show comparable exome sequencing results between FFPE and corresponding fresh-frozen cancer tissues using SOLiD sequencing. A prior study has been conducted comparing the validity of sequencing of FFPE vs. fresh frozen samples using other NGS platforms. Our validation further proves that FFPE material is a reliable source of material for whole exome sequencing.

  19. Zeemanfit: Use and Development of the solis_vms_zeemanfit code

    CERN Document Server

    Hughes, Anna L H; Marble, Andrew R; Pevtsov, Alexei A

    2013-01-01

    The purpose of the SOLIS Zeemanfit Code is to provide a straight-forward, easily checked measure of the total magnetic-field strength in the high-strength umbral regions of the solar disk. In the highest-strength regions, the Zeeman splitting of the 6302-angstrom Fe line becomes wide enough for the triplet nature of the line to be visible by eye in non-polarized light. Therefore, a three-line fit to the spectra should, in principle, provide a fairly robust measure of the total magnetic-field strength. The code uses the Level-1.5 spec-cube data of the SOLIS VSM 6302-vector observations (specifically the Stokes-I and Stokes-V components) to fit the line profiles at each appropriate pixel and calculate the magnetic-field-strength from the line-center separation of the two fit 6302.5 sigma-components. The 6301.5-angstrom Fe line is also present and fit in the VSM 6302-vector data, but it is an anomalous-Zeeman line with a weaker response to magnetic fields. Therefore, no magnetic- field measure is derived from th...

  20. Researcher Profile: An Interview with Virginia Solis Zuiker, Ph.D.

    Directory of Open Access Journals (Sweden)

    Virginia Solis Zuiker

    2015-12-01

    Full Text Available Virginia Solis Zuiker is an Associate Professor in the Department of Family Social Science at the University of Minnesota. She teaches courses on personal and family finance, family financial counseling, family resource management, economic perspectives of families, and family decision-making. Her scholarly research focus is in the area of economic well-being of families with particular interest in self-employment and family-owned businesses. Her research focuses on the Hispanic family life and she is the author of “Hispanic Self-Employment in the Southwest: Rising Above the Threshold of Poverty,” (Garland Publishing, 1997. She received her B.S. from the University of North Texas, an M.S. from Texas Tech University, and a Ph.D. from The Ohio State University. She served three years on the Board of Directors for the Association of Financial Counseling and Planning Education.

  1. Depth and Morphology of Wrinkle Ridge Detachments at Solis Planum, Mars

    Science.gov (United States)

    Colton, S. L.; Ferrill, D. A.; Smart, K. J.

    2005-12-01

    Wrinkle ridges -- long, linear to sinuous anticlines separated by relatively broad, flat synclinal valleys -- are a fundamental component of Martian geomorphology. The anticlinal crests show variable morphologies, but are often characterized by weak to strong asymmetry with variable vergence directions between adjacent ridges and along strike for any given ridge. Although wrinkle ridges are typically interpreted as contractional features, there is ongoing debate about their underlying structure and whether thrust faults penetrate to tens of kilometers of depth ("thick-skinned shortening") or sole into a detachment in the upper few kilometers of the Martian crust ("thin-skinned shortening"). Previous workers have estimated depth to the detachment horizon using a variety of methods including gravity inversion, geometry of crater-ridge intersections, mechanical modeling, and geometric modeling. Here we use a well-established terrestrial technique to calculate depth to the detachment horizon for wrinkle ridges in the Solis Planum region of Mars. We interpolate topographic profiles perpendicular to the regional trend of wrinkle ridges from Mars Orbiter Laser Altimeter (MOLA) Mission Experiment Gridded Data Record (MEGDR) altimetry data, set vertical reference lines on both sides of the ridge that define the limits of our measurement range, estimate the topographic surface prior to wrinkle ridge formation, and calculate the area uplifted above the original topographic surface. Dividing this excess area by the amount of shortening (the topographic profile length minus the length prior to deformation), provides depth to detachment. We calibrate the results with profiles from the less spatially-extensive but greater along-track density MOLA Precision Experiment Data Record (PEDR). Additional topographic and structural interpretation and analysis of wrinkle ridge morphology are conducted with Mars Orbiter Camera (MOC), Thermal Emission Imaging System (THEMIS), and High

  2. Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris).

    Science.gov (United States)

    Kim, Dong-Uk; Ka, Jong-Ok

    2014-03-01

    A bacterial strain, designated 5N26(T), was isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Cells of this strain were Gram-reaction-negative, strictly aerobic, motile, non-spore-forming rods, and catalase- and urease-negative. The major fatty acids of strain 5N26(T) were C16 : 0 (7.5 %), C18 : 1 2-OH (13.4 %) and summed feature 8 (C18:1ω6c and/or C18:1ω7c; 63.2%). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine and one unidentified aminolipid. The G+C content of the genomic DNA of strain 5N26(T) was 68.3 mol%. 16S rRNA gene sequence analysis showed that strain 5N26(T) was phylogenetically related to Roseomonas lacus TH-G33(T) and Roseomonas terrae DS-48(T) (97.0 % and 96.6 % sequence similarity, respectively). The results of genotypic and phenotypic data showed that strain 5N26(T) could be distinguished from phylogenetically related species, and that this strain represented a novel species within the genus Roseomonas, for which the name Roseomonas soli sp. nov. (type strain 5N26(T) = KACC 16376(T) = NBRC 109097(T)) is proposed.

  3. Geobacter soli sp. nov., a dissimilatory Fe(III)-reducing bacterium isolated from forest soil.

    Science.gov (United States)

    Zhou, Shungui; Yang, Guiqin; Lu, Qin; Wu, Min

    2014-11-01

    A novel Fe(III)-reducing bacterium, designated GSS01(T), was isolated from a forest soil sample using a liquid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. Cells of strain GSS01(T) were strictly anaerobic, Gram-stain-negative, motile, non-spore-forming and slightly curved rod-shaped. Growth occurred at 16-40 °C and optimally at 30 °C. The DNA G+C content was 60.9 mol%. The major respiratory quinone was MK-8. The major fatty acids were C(16:0), C(18:0) and C(16:1)ω7c/C(16:1)ω6c. Strain GSS01(T) was able to grow with ferrihydrite, Fe(III) citrate, Mn(IV), sulfur, nitrate or anthraquinone-2,6-disulfonate, but not with fumarate, as sole electron acceptor when acetate was the sole electron donor. The isolate was able to utilize acetate, ethanol, glucose, lactate, butyrate, pyruvate, benzoate, benzaldehyde, m-cresol and phenol but not toluene, p-cresol, propionate, malate or succinate as sole electron donor when ferrihydrite was the sole electron acceptor. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSS01(T) was most closely related to Geobacter sulfurreducens PCA(T) (98.3% sequence similarity) and exhibited low similarities (94.9-91.8%) to the type strains of other species of the genus Geobacter. The DNA-DNA relatedness between strain GSS01(T) and G. sulfurreducens PCA(T) was 41.4 ± 1.1%. On the basis of phylogenetic analysis, phenotypic characterization and physiological tests, strain GSS01(T) is believed to represent a novel species of the genus Geobacter, and the name Geobacter soli sp. nov. is proposed. The type strain is GSS01(T) ( =KCTC 4545(T) =MCCC 1K00269(T)).

  4. Comparison of three targeted enrichment strategies on the SOLiD sequencing platform.

    Directory of Open Access Journals (Sweden)

    Dale J Hedges

    Full Text Available Despite the ever-increasing throughput and steadily decreasing cost of next generation sequencing (NGS, whole genome sequencing of humans is still not a viable option for the majority of genetics laboratories. This is particularly true in the case of complex disease studies, where large sample sets are often required to achieve adequate statistical power. To fully leverage the potential of NGS technology on large sample sets, several methods have been developed to selectively enrich for regions of interest. Enrichment reduces both monetary and computational costs compared to whole genome sequencing, while allowing researchers to take advantage of NGS throughput. Several targeted enrichment approaches are currently available, including molecular inversion probe ligation sequencing (MIPS, oligonucleotide hybridization based approaches, and PCR-based strategies. To assess how these methods performed when used in conjunction with the ABI SOLID3+, we investigated three enrichment techniques: Nimblegen oligonucleotide hybridization array-based capture; Agilent SureSelect oligonucleotide hybridization solution-based capture; and Raindance Technologies' multiplexed PCR-based approach. Target regions were selected from exons and evolutionarily conserved areas throughout the human genome. Probe and primer pair design was carried out for all three methods using their respective informatics pipelines. In all, approximately 0.8 Mb of target space was identical for all 3 methods. SOLiD sequencing results were analyzed for several metrics, including consistency of coverage depth across samples, on-target versus off-target efficiency, allelic bias, and genotype concordance with array-based genotyping data. Agilent SureSelect exhibited superior on-target efficiency and correlation of read depths across samples. Nimblegen performance was similar at read depths at 20× and below. Both Raindance and Nimblegen SeqCap exhibited tighter distributions of read depth

  5. Rheinheimera soli sp. nov., a gammaproteobacterium isolated from soil in Korea.

    Science.gov (United States)

    Ryu, Seung Hyun; Chung, Bok Sil; Park, Minjeong; Lee, Seung Sik; Lee, Sang-Suk; Jeon, Che Ok

    2008-10-01

    A Gram-negative, non-spore-forming bacterium, designated strain BD-d46(T), was isolated from a playground soil sample in Jinju, South Korea. Cells were straight or curved rods and showed catalase- and oxidase-positive reactions. Growth of strain BD-d46(T) was observed between 15 and 35 degrees C (optimum 25-30 degrees C) and between pH 6.5 and 8.0 (optimum pH 7.0-7.5). The predominant fatty acids were summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), C(12 : 0) 3-OH and C(16 : 0). Strain BD-d46(T) contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Isoprenoid quinones were Q-8 (75 %) and MK-7 (25 %). The G+C content of the genomic DNA was 49.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-d46(T) formed a distinct lineage with Rheinheimera chironomi K19414(T) within the genus Rheinheimera. Levels of 16S rRNA gene sequence similarity between strain BD-d46(T) and the type strains of recognized Rheinheimera species ranged from 94.4 to 96.9 %. On the basis of chemotaxonomic data and molecular properties, strain BD-d46(T) is considered to represent a novel species of the genus Rheinheimera, for which the name Rheinheimera soli sp. nov. is proposed. The type strain is BD-d46(T) (=KCTC 22077(T) =DSM 19413(T)).

  6. Fine de novo sequencing of a fungal genome using only SOLiD short read data: verification on Aspergillus oryzae RIB40.

    Directory of Open Access Journals (Sweden)

    Myco Umemura

    Full Text Available The development of next-generation sequencing (NGS technologies has dramatically increased the throughput, speed, and efficiency of genome sequencing. The short read data generated from NGS platforms, such as SOLiD and Illumina, are quite useful for mapping analysis. However, the SOLiD read data with lengths of <60 bp have been considered to be too short for de novo genome sequencing. Here, to investigate whether de novo sequencing of fungal genomes is possible using only SOLiD short read sequence data, we performed de novo assembly of the Aspergillus oryzae RIB40 genome using only SOLiD read data of 50 bp generated from mate-paired libraries with 2.8- or 1.9-kb insert sizes. The assembled scaffolds showed an N50 value of 1.6 Mb, a 22-fold increase than those obtained using only SOLiD short read in other published reports. In addition, almost 99% of the reference genome was accurately aligned by the assembled scaffold fragments in long lengths. The sequences of secondary metabolite biosynthetic genes and clusters, whose products are of considerable interest in fungal studies due to their potential medicinal, agricultural, and cosmetic properties, were also highly reconstructed in the assembled scaffolds. Based on these findings, we concluded that de novo genome sequencing using only SOLiD short reads is feasible and practical for molecular biological study of fungi. We also investigated the effect of filtering low quality data, library insert size, and k-mer size on the assembly performance, and recommend for the assembly use of mild filtered read data where the N50 was not so degraded and the library has an insert size of ∼2.0 kb, and k-mer size 33.

  7. Decoding the Hermetic Discourse in Salomon Trismosin's Splendor Solis - A Semiotic Study of Three Ways of Reading

    Directory of Open Access Journals (Sweden)

    J. Peter Södergård

    1996-01-01

    Full Text Available Alchemy and the Hermetic Art are terms that denote a most interesting transitional space, circumscribing an opaque region of human cultural history, shared between matter and psyche, between phantasmagoric reveries and practical experiments, between sincere natural theology and conscious fraud. This is an area of human experience that has been notoriously difficult to define and understand, and which has triggered off contradictory interpretations that are, in their own, of semiotic interest. It has been said that a text — in our case Splendor Solis, and the Hermetic–alchemical texts at large — is a picnic, a Dutch party, in which the author provides the words and the reader, especially a reader with too much or too little erudition, comes up with the meaning of the words. In this article, the author tries to give an overview of the general interpretations of alchemy that have been put forward, and apply Umberto Eco's intentional approach to these interpretations, viz. consider them as various sorts of intentions. The author uses a pseudonymous alchemical tractate from the sixteenth century, Salomon Trismosin's treatise Splendor Solis as an opportunity to picture and evaluate the three main decodings of alchemy that have been attempted; a chemical, a religious—soteriological and a psychological. The author also explores the intertextual web in the Hermetic discourse, and see if its mapping can generate usable knowledge about what has, since the Middle Ages, been called alchemy: a term acknowledging the taking up of a science or art which had been cultivated by Muslim and Nestorian scholars, but with its fountain head in Late Antiquity.

  8. Categorical Analysis of Human T Cell Heterogeneity with One-Dimensional Soli-Expression by Nonlinear Stochastic Embedding.

    Science.gov (United States)

    Cheng, Yang; Wong, Michael T; van der Maaten, Laurens; Newell, Evan W

    2016-01-15

    Rapid progress in single-cell analysis methods allow for exploration of cellular diversity at unprecedented depth and throughput. Visualizing and understanding these large, high-dimensional datasets poses a major analytical challenge. Mass cytometry allows for simultaneous measurement of >40 different proteins, permitting in-depth analysis of multiple aspects of cellular diversity. In this article, we present one-dimensional soli-expression by nonlinear stochastic embedding (One-SENSE), a dimensionality reduction method based on the t-distributed stochastic neighbor embedding (t-SNE) algorithm, for categorical analysis of mass cytometry data. With One-SENSE, measured parameters are grouped into predefined categories, and cells are projected onto a space composed of one dimension for each category. In contrast with higher-dimensional t-SNE, each dimension (plot axis) in One-SENSE has biological meaning that can be easily annotated with binned heat plots. We applied One-SENSE to probe relationships between categories of human T cell phenotypes and observed previously unappreciated cellular populations within an orchestrated view of immune cell diversity. The presentation of high-dimensional cytometric data using One-SENSE showed a significant improvement in distinguished T cell diversity compared with the original t-SNE algorithm and could be useful for any high-dimensional dataset.

  9. Two new species of dicyemid mesozoans (Dicyemida: Dicyemidae) from Octopus maya Voss & Solis-Ramirez (Octopodidae) off Yucatan, Mexico.

    Science.gov (United States)

    Castellanos-Martinez, Sheila; Aguirre-Macedo, M Leopoldina; Furuya, Hidetaka

    2016-07-01

    Two new dicyemid species are described from the endemic cephalopod Octopus maya Voss & Solis-Ramirez collected off Yucatan, Mexico. The renal sacs of 40 juvenile and adult octopuses from four localities were examined. Dicyema hochbergi n. sp. is a medium-sized species that reaches 2,245 µm in length. The vermiform stages consist of 18-24 peripheral cells, a conical calotte and the extension of the axial cell between the base and middle of the metapolar cells. Infusoriform embryos consist of 39 cells with urn cell containing one germinal cell, two nuclei and solid refringent bodies. Dicyema mexcayae n. sp. is a relatively small species that reaches 1,114 µm in length. The vermiform stages are constituted by 14-16 peripheral cells, an elongate calotte and the axial cell extending forward to the middle of the metapolar cells. The infusoriform embryos consist of 37 cells, two solid refringent bodies and urn cells with two nuclei each. The present study represents the first description of a dicyemid species from O. maya and increases the number of described species from Mexican waters to 11.

  10. Optimization of Bartonella henselae multilocus sequence typing scheme using single-nucleotide polymorphism analysis of SOLiD sequence data

    Institute of Scientific and Technical Information of China (English)

    ZHAO Fan; Gemma Chaloner; Alistair Darby; SONG Xiu-ping; LI Dong-mei; Richard Birtles; LIU Qi-yong

    2012-01-01

    Background Multi-locus sequence typing (MLST) is widely used to explore the population structure of numerous bacterial pathogens.However,for genotypically-restricted pathogens,the sensitivity of MLST is limited by a paucity of variation within selected loci.For Bartonella henselae (B.henselae),although the MLST scheme currently used has been proven useful in defining the overall population structure of the species,its reliability for the accurate delineation of closely-related sequence types,between which allelic variation is usually limited to,at most,one or two nucleotide polymorphisms.Exploitation of high-throughput sequencing data allows a more informed selection of MLST loci and thus,potentially,a means of enhancing the sensitivity of the schemes they comprise.Methods We carried out SOLiD resequencing on 12 representative B.henselae isolates and explored these data using single nucleotide polymorphism (SNP) analysis.We determined the number and distribution of SNPs in the genes targeted by the established MLST scheme and modified the position of loci within these genes to capture as much genetic variation as possible.Results Using genome-wide SNP data,we found the distribution of SNPs within each open reading frame (ORF) of MLST loci,which were not represented by the established B.henselae MLST scheme.We then modified the position of loci in the MLST scheme to better reflect the polymorphism in the ORF as a whole.The use of amended loci in this scheme allowed previously indistinguishable ST1 strains to be differentiated.However,the diversity of B.henselae was still rare in China.Conclusions Our study demonstrates the use of SNP analysis to facilitate the selection of MLST loci to augment the currently-described scheme for B.henselae.And the diversity among B.henselae strains in China is markedly less than that observed in B.henselae populations elsewhere in the world.

  11. Transcription Profiling of the Model Cyanobacterium Synechococcus sp. Strain PCC 7002 by Next-Gen (SOLiD™) Sequencing of cDNA

    OpenAIRE

    Ludwig, Marcus; Bryant, Donald A.

    2011-01-01

    The genome of the unicellular, euryhaline cyanobacterium Synechococcus sp. PCC 7002 encodes about 3200 proteins. Transcripts were detected for nearly all annotated open reading frames by a global transcriptomic analysis by Next-Generation (SOLiD™) sequencing of cDNA. In the cDNA samples sequenced, ∼90% of the mapped sequences were derived from the 16S and 23S ribosomal RNAs and ∼10% of the sequences were derived from mRNAs. In cells grown photoautotrophically under standard conditions [38°C, ...

  12. Transcription profiling of the model cyanobacterium Synechococcus sp. strain PCC 7002 by NextGen (SOLiD™) Sequencing of cDNA

    OpenAIRE

    Marcus eLudwig; Bryant, Donald A.

    2011-01-01

    The genome of the unicellular, euryhaline cyanobacterium Synechococcus sp. PCC 7002 encodes about 3200 proteins. Transcripts were detected for nearly all annotated open reading frames by a global transcriptomic analysis by Next-Generation (SOLiDTM) sequencing of cDNA. In the cDNA samples sequenced, ~90% of the mapped sequences were derived from the 16S and 23S ribosomal RNAs and ~10% of the sequences were derived from mRNAs. In cells grown photoautotrophically under standard conditions (38 &#...

  13. Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands.

    Science.gov (United States)

    Heyrman, Jeroen; Vanparys, Bram; Logan, Niall A; Balcaen, An; Rodríguez-Díaz, Marina; Felske, Andreas; De Vos, Paul

    2004-01-01

    A group of 42 isolates were isolated from the soil of several disused hay fields, in the Drentse A agricultural research area (The Netherlands), that were taken out of production at different times. The group represents hitherto-uncultured Bacillus lineages that have previously been found, by a non-cultural method, to be predominant in soil. The strains were subjected to a polyphasic taxonomic study, including (GTG)5-PCR, 16S rDNA sequence analysis, DNA-DNA hybridizations, DNA base-ratio determination, fatty acid analysis and morphological and biochemical characterization. By comparing the groupings obtained by (GTG)5-PCR and 16S rDNA sequence analysis, six clusters of similar strains could be recognized. A DNA-DNA relatedness study showed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus, namely Bacillus novalis sp. nov. (type strain IDA3307T=R-15439T=LMG 21837T=DSM 15603T), Bacillus vireti sp. nov. (type strain IDA3632T=R-15447T=LMG 21834T=DSM 15602T), Bacillus soli sp. nov. (type strain IDA0086T=R-16300T=LMG 21838T=DSM 15604T), Bacillus bataviensis sp. nov. (type strain IDA1115T=R-16315T=LMG 21833T=DSM 15601T) and Bacillus drentensis sp. nov. (type strain IDA1967T=R-16337T=LMG 21831T=DSM 15600T).

  14. First Synoptic Maps of Photospheric Vector Magnetic Field from SOLIS/VSM: Non-Radial Magnetic Fields and Hemispheric Pattern of Helicity

    CERN Document Server

    Gosain, S; Rudenko, G V; Anfinogentov, S A

    2013-01-01

    We use daily full-disk vector magnetograms from Vector Spectromagnetograph (VSM) on Synoptic Optical Long-term Investigations of the Sun (SOLIS) system to synthesize the first Carrington maps of the photospheric vector magnetic field. We describe these maps and make a comparison of observed radial field with the radial field estimate from LOS magnetograms. Further, we employ these maps to study the hemispheric pattern of current helicity density, Hc, during the rising phase of the solar cycle 24. Longitudinal average over the 23 consecutive solar rotations shows a clear signature of the hemispheric helicity rule, i.e. Hc is predominantly negative in the North and positive in South. Although our data include the early phase of cycle 24, there appears no evidence for a possible (systematic) reversal of the hemispheric helicity rule at the beginning of cycle as predicted by some dynamo models. Further, we compute the hemispheric pattern in active region latitudes (-30 deg \\le \\theta \\le 30 deg) separately for we...

  15. Transcription profiling of the model cyanobacterium Synechococcus sp. strain PCC 7002 by NextGen (SOLiD™ Sequencing of cDNA

    Directory of Open Access Journals (Sweden)

    Marcus eLudwig

    2011-03-01

    Full Text Available The genome of the unicellular, euryhaline cyanobacterium Synechococcus sp. PCC 7002 encodes about 3200 proteins. Transcripts were detected for nearly all annotated open reading frames by a global transcriptomic analysis by Next-Generation (SOLiDTM sequencing of cDNA. In the cDNA samples sequenced, ~90% of the mapped sequences were derived from the 16S and 23S ribosomal RNAs and ~10% of the sequences were derived from mRNAs. In cells grown photoautotrophically under standard conditions (38 °C, 1% (v/v CO2 in air, 250 µmol photons m-2 s-1, the highest transcript levels (up to 2% of the total mRNA for the most abundantly transcribed genes (e. g., cpcAB, psbA, psaA were generally derived from genes encoding structural components of the photosynthetic apparatus. High light exposure for one hour caused changes in transcript levels for genes encoding proteins of the photosynthetic apparatus, Type-1 NADH dehydrogenase complex and ATP synthase, whereas dark incubation for one hour resulted in a global decrease in transcript levels for photosynthesis-related genes and an increase in transcript levels for genes involved in carbohydrate degradation. Transcript levels for pyruvate kinase and the pyruvate dehydrogenase complex decreased sharply in cells incubated in the dark. Under dark anoxic (fermentative conditions, transcript changes indicated a global decrease in transcripts for respiratory proteins and suggested that cells employ an alternative phosphoenolpyruvate degradation pathway via phosphoenolpyruvate synthase (ppsA and the pyruvate:ferredoxin oxidoreductase (nifJ. Finally, the data suggested that an apparent operon involved in tetrapyrrole biosynthesis and fatty acid desaturation, acsF2-ho2-hemN2-desF, may be regulated by oxygen concentration.

  16. SOLiD™ sequencing of genomes of clinical isolates of Leishmania donovani from India confirm leptomonas co-infection and raise some key questions.

    Directory of Open Access Journals (Sweden)

    Neeloo Singh

    Full Text Available BACKGROUND: Known as 'neglected disease' because relatively little effort has been applied to finding cures, leishmaniasis kills more than 150,000 people every year and debilitates millions more. Visceral leishmaniasis (VL, also called Kala Azar (KA or black fever in India, claims around 20,000 lives every year. Whole genome analysis presents an excellent means to identify new targets for drugs, vaccine and diagnostics development, and also provide an avenue into the biological basis of parasite virulence in the L. donovani complex prevalent in India. METHODOLOGY/PRINCIPAL FINDINGS: In our presently described study, the next generation SOLiD™ platform was successfully utilized for the first time to carry out whole genome sequencing of L. donovani clinical isolates from India. We report the exceptional occurrence of insect trypanosomatids in clinical cases of visceral leishmaniasis (Kala Azar patients in India. We confirm with whole genome sequencing analysis data that isolates which were sequenced from Kala Azar (visceral leishmaniasis cases were genetically related to Leptomonas. The co-infection in splenic aspirate of these patients with a species of Leptomonas and how likely is it that the infection might be pathogenic, are key questions which need to be investigated. We discuss our results in the context of some important probable hypothesis in this article. CONCLUSIONS/SIGNIFICANCE: Our intriguing results of unusual cases of Kala Azar found to be most similar to Leptomonas species put forth important clinical implications for the treatment of Kala Azar in India. Leptomonas have been shown to be highly susceptible to several standard leishmaniacides in vitro. There is very little divergence among these two species viz. Leishmania sp. and L. seymouri, in terms of genomic sequence and organization. A more extensive perception of the phenomenon of co-infection needs to be addressed from molecular pathogenesis and eco

  17. SOLiD-SAGE of endophyte-infected red fescue reveals numerous effects on host transcriptome and an abundance of highly expressed fungal secreted proteins.

    Directory of Open Access Journals (Sweden)

    Karen V Ambrose

    Full Text Available One of the most important plant-fungal symbiotic relationships is that of cool season grasses with endophytic fungi of the genera Epichloë and Neotyphodium. These associations often confer benefits, such as resistance to herbivores and improved drought tolerance, to the hosts. One benefit that appears to be unique to fine fescue grasses is disease resistance. As a first step towards understanding the basis of the endophyte-mediated disease resistance in Festuca rubra we carried out a SOLiD-SAGE quantitative transcriptome comparison of endophyte-free and Epichloë festucae-infected F. rubra. Over 200 plant genes involved in a wide variety of physiological processes were statistically significantly differentially expressed between the two samples. Many of the endophyte expressed genes were surprisingly abundant, with the most abundant fungal tag representing over 10% of the fungal mapped tags. Many of the abundant fungal tags were for secreted proteins. The second most abundantly expressed fungal gene was for a secreted antifungal protein and is of particular interest regarding the endophyte-mediated disease resistance. Similar genes in Penicillium and Aspergillus spp. have been demonstrated to have antifungal activity. Of the 10 epichloae whole genome sequences available, only one isolate of E. festucae and Neotyphodium gansuense var inebrians have an antifungal protein gene. The uniqueness of this gene in E. festucae from F. rubra, its transcript abundance, and the secreted nature of the protein, all suggest it may be involved in the disease resistance conferred to the host, which is a unique feature of the fine fescue-endophyte symbiosis.

  18. SOLiD-SAGE of endophyte-infected red fescue reveals numerous effects on host transcriptome and an abundance of highly expressed fungal secreted proteins.

    Science.gov (United States)

    Ambrose, Karen V; Belanger, Faith C

    2012-01-01

    One of the most important plant-fungal symbiotic relationships is that of cool season grasses with endophytic fungi of the genera Epichloë and Neotyphodium. These associations often confer benefits, such as resistance to herbivores and improved drought tolerance, to the hosts. One benefit that appears to be unique to fine fescue grasses is disease resistance. As a first step towards understanding the basis of the endophyte-mediated disease resistance in Festuca rubra we carried out a SOLiD-SAGE quantitative transcriptome comparison of endophyte-free and Epichloë festucae-infected F. rubra. Over 200 plant genes involved in a wide variety of physiological processes were statistically significantly differentially expressed between the two samples. Many of the endophyte expressed genes were surprisingly abundant, with the most abundant fungal tag representing over 10% of the fungal mapped tags. Many of the abundant fungal tags were for secreted proteins. The second most abundantly expressed fungal gene was for a secreted antifungal protein and is of particular interest regarding the endophyte-mediated disease resistance. Similar genes in Penicillium and Aspergillus spp. have been demonstrated to have antifungal activity. Of the 10 epichloae whole genome sequences available, only one isolate of E. festucae and Neotyphodium gansuense var inebrians have an antifungal protein gene. The uniqueness of this gene in E. festucae from F. rubra, its transcript abundance, and the secreted nature of the protein, all suggest it may be involved in the disease resistance conferred to the host, which is a unique feature of the fine fescue-endophyte symbiosis.

  19. High prevalence of blaOXA-23 in Acinetobacter spp. and detection of blaNDM-1 in A. soli in Cuba: report from National Surveillance Program (2010–2012)

    Science.gov (United States)

    Quiñones, D.; Carvajal, I.; Perez, Y.; Hart, M.; Perez, J.; Garcia, S.; Salazar, D.; Ghosh, S.; Kawaguchiya, M.; Aung, M.S.; Kobayashi, N.

    2015-01-01

    As a first national surveillance of Acinetobacter in Cuba, a total of 500 Acinetobacter spp. isolates recovered from 30 hospitals between 2010 and 2012 were studied. Acinetobacter baumannii–calcoaceticus complex accounted for 96.4% of all the Acinetobacter isolates, while other species were detected at low frequency (A. junii 1.6%, A. lwoffii 1%, A. haemolyticus 0.8%, A. soli 0.2%). Resistance rates of isolates were 34–61% to third-generation cephalosporins, 49–50% to β-lactams/inhibitor combinations, 42–47% to aminoglycosides, 42–44% to carbapenems and 55% to ciprofloxacin. However, resistance rates to colistin, doxycycline, tetracycline and rifampin were less than 5%. Among carbapenem-resistant isolates, 75% harboured different blaOXA genes (OXA-23, 73%; OXA-24, 18%; OXA-58, 3%). The blaNDM-1 gene was identified in an A. soli strain, of which the species was confirmed by sequence analysis of 16S rRNA gene, rpoB, rpoB–rpoC and rpoL–rpoB intergenic spacer regions and gyrB. The sequences of blaNDM-1 and its surrounding genes were identical to those reported for plasmids of A. baumannii and A. lwoffi strains. This is the first report of blaNDM-1 in A. soli, together with a high prevalence of OXA-23 carbapenemase for carbapenem resistance in Acinetobacter spp. in Cuba. PMID:26236494

  20. High prevalence of blaOXA-23 in Acinetobacter spp. and detection of blaNDM-1 in A. soli in Cuba: report from National Surveillance Program (2010–2012

    Directory of Open Access Journals (Sweden)

    D. Quiñones

    2015-09-01

    Full Text Available As a first national surveillance of Acinetobacter in Cuba, a total of 500 Acinetobacter spp. isolates recovered from 30 hospitals between 2010 and 2012 were studied. Acinetobacter baumannii–calcoaceticus complex accounted for 96.4% of all the Acinetobacter isolates, while other species were detected at low frequency (A. junii 1.6%, A. lwoffii 1%, A. haemolyticus 0.8%, A. soli 0.2%. Resistance rates of isolates were 34–61% to third-generation cephalosporins, 49–50% to β-lactams/inhibitor combinations, 42–47% to aminoglycosides, 42–44% to carbapenems and 55% to ciprofloxacin. However, resistance rates to colistin, doxycycline, tetracycline and rifampin were less than 5%. Among carbapenem-resistant isolates, 75% harboured different blaOXA genes (OXA-23, 73%; OXA-24, 18%; OXA-58, 3%. The blaNDM-1 gene was identified in an A. soli strain, of which the species was confirmed by sequence analysis of 16S rRNA gene, rpoB, rpoB–rpoC and rpoL–rpoB intergenic spacer regions and gyrB. The sequences of blaNDM-1 and its surrounding genes were identical to those reported for plasmids of A. baumannii and A. lwoffi strains. This is the first report of blaNDM-1 in A. soli, together with a high prevalence of OXA-23 carbapenemase for carbapenem resistance in Acinetobacter spp. in Cuba.

  1. Ius sanguinis – ius soli.

    OpenAIRE

    Sanabria León, Jorge

    2012-01-01

    El hospital gozaba de la misma arquitectura de las barracas y las viviendas de gamonales en las compañías bananeras para el calor de las zonas costeñas, solo que a casi tres mil metros sobre el nivel del mar; igual que el Ejército de Salvación, un par de cuadras hacia el este

  2. Renovation of Shaft Mining Building No. 2 in Kłodawa Salt Mine/ Renowacja Budynku Nadszybia Nr 2 Na Terenie Kopalni Soli "Kłodawa" S.A.

    Science.gov (United States)

    Błaszczyński, Tomasz; Wielentejczyk, Przemysław

    2015-06-01

    The paper presents the renovation process of the shaft mining building No. 2 situated in the Kłodawa Salt Mine. A technical state of the facility required immediate reinforcement of structural elements, which was confirmed by expertise carried out by the authors. A lack of repairs could be the cause of building damage. The progress of corrosion in some steel profiles of columns or floors was very advanced. The state of the building was rapidly worsening due to the very high salinity of the indoor environment, moisture (building not insulated) and vibrating engines of machinery operating on different floors felt throughout the facility. After carrying out the technical expertise, working plans and specifications, and relevant numerical analysis, the modernization process was realized by the reinforcement or rebuilding of structural elements. Referat przedstawia sposób remontu i naprawy konstrukcji nośnej i obudowy budynku nadszybia nr 2 na terenie Kopalni Soli "Kłodawa". Elementy konstrukcyjne budynku wymagały natychmiastowego wzmocnienia, co potwierdziła ekspertyza wykonana przez autorów. Zaniechanie prac remontowych groziło awarią budowlaną pomimo przeprowadzonej wcześniej w 2002 r. naprawy. Postęp korozji w niektórych profilach stalowych słupów czy stropów był bardzo zaawansowany. Stan obiektu pogarszał się szybko ze względu na bardzo duże zasolenie środowiska, wilgoć (budynek nieocieplony) oraz pracujące na poszczególnych poziomach maszyny wytwarzające duże drgania wyczuwalne w każdym miejscu obiektu. Wykonano ekspertyzę i projekt wykonawczy przed przystąpieniem do prac renowacyjnych. Przeprowadzono stosowną analizę numeryczną budynku uwzględniając w obliczeniach osłabione korozją elementy pomniejszając odpowiednio w modelu ich parametry wytrzymałościowe. Na tej podstawie zaproponowano stosowne wzmocnienia. Zastosowano technologie wzmocnień stosując wymianę profili stalowych, wspawywanie dodatkowych profili lub blach

  3. Clinical outcomes of Solis implantation for type Ⅱ and Ⅱ a Hangman's fractures%应用Solis融合器置入治疗Ⅱ型及Ⅱa型Hangman骨折的临床疗效

    Institute of Scientific and Technical Information of China (English)

    黄阳亮; 刘少喻; 梁春祥; 李浩淼; 于滨生; 龙厚清; 韩国伟; 张旭华; 魏富鑫

    2012-01-01

    目的:评价Solis椎间融合器置入治疗Ⅱ型及Ⅱa型Hangman骨折的临床疗效.方法:2006年1月~2011年11月,我科采用经前路Solis椎间融合器置入治疗Ⅱ型及Ⅱa型Hangman骨折患者17例,术中应用颈椎牵开器牵开椎间,通过紧张韧带进一步复位骨折.其中男11例、女6例,年龄19~41岁,平均26.2岁,按Levine-Edwards分类法Ⅱ型11例,Ⅱa型6例.于术后1周、3个月、6个月及末次随访时复查X线片,记录术前及末次随访时的枢椎移位及成角数据,记录术前及末次随访时的创伤后颈椎临床评分、颈部疼痛的VAS评分观察临床疗效.术前与末次随访时各数据间行t检验.结果:手术时间平均为78min (55~135min);术中出血量平均为22.5ml (10~50ml).术中无喉上神经、咽喉壁及血管损伤,术后无喉头水肿、血肿形成及伤口感染等手术并发症.平均随访25.1个月(6~48个月),手术节段及骨折部位平均于3个月时骨性融合,未见畸形愈合,末次随访时融合器无下沉、无移位、椎间无塌陷.创伤后颈椎临床评分:术前平均为48分(45~63分),末次随访时为92分(83~97分);颈部疼痛VAS评分术前平均为6.9分(6~9分),末次随访时为1.1分(0~2分);枢椎成角术前平均为-8.6°(-20~6°),末次随访时为+3.5°(0~+8°);枢椎移位术前平均为3.4mm( 1~5.5mm),末次随访时为1.2mm(0~2.0mm).以上两组数据间差异均有统计学意义(P<0.05).结论:应用Solis椎间融合器置入治疗Hangman骨折可行,手术操作简单、耗时短、创伤小、融合率高且有利于颈椎稳定.%Objectives: To investigate the clinical outcomes of Solis implantation for type II and II a Hangman's fractures. Methods: From January 2006 to November 2011, a total of 17 cases with type II and II a Hangman's fractures underwent anterior Solis placement. There were 11 males and 6 females, with the average age of 26.2 (range, 19-41). The reduction was achieved by

  4. Ako klamať pomocou štatistiky

    OpenAIRE

    Štefan, Pavol

    2014-01-01

    This paper describes the misleading in statistical research and its incorrect presentation. It concentrates on presentation of statistical research, what is the most common source of misunderstanding of statistical results by public and by professionals. The basic factors, which influence the statistical research, is described within theoretical part of this paper. The particular examples, which demonstrates incorrect statistical approach and misleading presentation of statistical research, i...

  5. Franchising ako možná forma podnikania

    OpenAIRE

    Kováčová, Katarína

    2013-01-01

    The Master Thesis describes franchising as a possible form of doing business and its application into reality. The goal is to sum up all the theoretical knowledge and to analyse the czech legislation that is related to franchise agreement. The theoretical part describes the overall character of franchising and a way of founding the franchise company. It also outlines the strenghts and weaknesses of franchising concerning both parties of this relationship. The practical part introduces a way h...

  6. Internet ako prostriedok komunikácie a propagácie firiem

    OpenAIRE

    Vongrejová, Alena

    2010-01-01

    The aim of the bachelor thesis was the practical ability to use emphirical datas about web page design and their application on chosen company. The thesis is divided into three chapters. The first chapter explains the concept of the internet communication and analyzes all its parts in detail. The second section deals with the evolution of the internet communication and the history of the Internet till the present days. The second chapter sums all the obtained information and technologies used...

  7. KOGNITÍVNE SKRESLENIA AKO SÚČASŤ SPRISAHANECKÝCH TEÓRIÍ

    Directory of Open Access Journals (Sweden)

    Imrich Ruisel

    2015-09-01

    Full Text Available The beginnings of modern Greek philosophy have already indicated that man is not a reliable information processor, because he is unable to completely encompass the real world at a cognitive level. Thus, already Plato distinguished between the knowledge of the real true belief (epistémé and opinion (doxa. F. Bacon assumed that human senses are not the measure of reality. Psychologists A. Tversky and D. Kahneman pointed out that people do not follow rational arguments and set rules in their judgments. Rather, they use heuristics, which are simplified judgment rules that enable quick judgments, often biased by individual’s subjective beliefs. These wrong conclusions induce false ideas or fallacies. There are deductive and inductive fallacies. We contemplate the ratio of cognitive fallacies in forming and existence of conspiracy theories. Deliberate use of fallacies and biases is characteristic of the use of conspiracy theories in propaganda. Wrong judgments are made due to wrong understanding of coincidental events, insufficient processing of the laws of statistical regression and the tendency to search for confirmation. Preferred are verbal techniques in the form of naive and ritualized terms and statements based on unidentifiable fallacies.

  8. Guerilla marketing ako nový trend v marketingovej komunikácii

    OpenAIRE

    Baraniaková, Júlia

    2010-01-01

    The goal of the bachelor thesis is to theoretically define guerrilla marketing as one of the new trends in marketing communication and to point out its advantages and disadvantages compared to traditional marketing communication. Moreover, the aim is to show its practical use on a company active on the Czech market (Nike CR) by individually interviewing its current marketing manager for CR and SR who explains the company's strategy in the field of guerilla marketing. Finally, the goal is to e...

  9. Ako marketingové aktivity ovplyvňujú stravovacie návyky u detí

    OpenAIRE

    Lipková, Jela

    2011-01-01

    This bachelor thesis aims to determine what is the impact of marketing activities on children, especially advertising, by creating the eating habits. The theoretical part deals with marketing communications and particular types of marketing communication tools. Further it focuses on the most famous communication tool, advertising and its effect on the children. In the theoretical part also deals with consumer behavior and factors that affect the individual consumers in the buying behavior, wh...

  10. Bankopoistenie ako súčasť českého finančného trhu

    OpenAIRE

    Pastiranová, Oľga

    2012-01-01

    The thesis focuses on the issue of bancassurance and comprehensive view on this area of financial market applied to the current market conditions in Czech Republic. The thesis defines the theoretical concept of bancassurance, the causes of its formation, advantages and disadvantages resulting from cross-industry integration of the banking and insurance sector and the ways of cooperation between them. The thesis also analyses and compares a chosen bancassurance product offered by the most sign...

  11. Vytvorenie internetového obchodu, ako nadstavba kamenného obchodu spoločnosti

    OpenAIRE

    Mokrý, Richard

    2012-01-01

    This diploma thesis concerns with electronic business. The technology of the trade, the internet and it's services and the history is described in the first chapter. Next chapter concerns with the general theoretical issues of electronic business. Defines relationships of particular subjects, such as client, seller or government service that appear in the terms and conditions of electronic business. Then describes the general model of electronic business. The third chapter is dedicated to gen...

  12. Úverové registre ako nástroj znižovania úverového rizika

    OpenAIRE

    Bohumel, Michal

    2013-01-01

    This thesis deals with the credit bureaus, followed by an analysis of credit bureaus in Slovak and Czech banking sector and an overview of the operation of credit bureaus of European countries. In the initial part of the thesis I explains key concepts such as credit bureaus, bank risk, credit risk, market risk, liquidity risk, operational risk. Then I deal with the issue of credit bureaus as an instrument of reducing credit risk. I discuss the importance of credit bureaus for banks and their ...

  13. Biotransformation of Ferulic acid to 4-Vinylguaiacol by Enterobacter soli and E. aerogenes

    Science.gov (United States)

    We investigated the conversion of ferulic acid to 4-vinylguaiacol (4-VG), vanillin, vanillyl alcohol and vanillic acid by five Enterobacter strains. These high-value chemicals are usually synthesized using chemical methods but biological synthesis adds value. Ferulic acid, a relatively inexpensive...

  14. El virrey Solis en las letras colombianas. Bibliografía

    Directory of Open Access Journals (Sweden)

    León Faidherbee Lyday

    1969-03-01

    Full Text Available En la historia de Nueva Granada y de la República de Colombia pocas figuras han llamado tanto la atención al historiador y al literato como José Solís Folch de Cardona, íntimo amigo de Fernando VI de España, Mariscal de Campo de los reales ejércitos, Comendador de la Orden de San Jorge de Montesa, cuarto virrey de Nueva Granada, y más tarde profeso de la orden monástica franciscana en la ciudad de Bogotá.

  15. The Prokaryotic Expression and Bioactivity of the Recombinant Red Fire Ant Venom Allergen Soli4

    Institute of Scientific and Technical Information of China (English)

    HAN Xue-qing; LIN Xiang-mei; CHEN Hong-jun; ZHANG Yong-guo; YE Gui-sheng; WU Shao-qiang; LI Jian; CHEN Nai-zhong; CHEN Yan; ZHU Shui-fang

    2009-01-01

    The sting of red imported fire ant (RIFA) could cause serious allergic response in fraction of people. These allergic reactions are mainly caused by its venom, especially venom allergen Sol I 1-4. To produce large amount of RIFA venom allergen Sol I 4 for diagnosis of RIFA allergy and allergen-specific immunotherapy, the gene encoding this protein was amplified and cloned into the prokaryotic expression vector pET43, la. The recombinant plasmid was used to transform competent cells and the recombinant proteins were expressed in E. Coli. SDS-PAGE and Western blotting analysis indicated that high-level expression of Sol I 4 protein was successfully achieved. Allergenic activity analysis of the recombinant allergen Sol I 4 was then performed on rabbit. The result showed that the recombinant protein obtained had significant allergenic activity. It indicated that the recombinant allergen Sol I 4 of RIFA venom was successfully expressed in E. Coli, which provided foundation for further developing therapeutic and diagnosis reagents of RIFA allergy.

  16. Priateľstvo ako cnosť súkromná i verejná (Friendship as a Private and Public Virtues

    Directory of Open Access Journals (Sweden)

    Matúš Porubjak

    2008-03-01

    Full Text Available The aim of this article is to show a social role of friendship (filia in Classical Greece and to discover why, as Aristotle thinks, is filia the greatest good for the polis. The author bases his analysis on a comparison between Plato’s dialogue Lysis and Aristotle’s Politics (namely the book 1 and book 3. He distinguishes four types of filia, and shows the social role played by each one of them: the love of parents to children, the friendship between teenage boys of the same age, the love of elder men to boys, and the friendship between free adult citizens.

  17. Peniaze ako zdroj šťastia v našich životoch? (Is Money a Source of Happiness in our lives?

    Directory of Open Access Journals (Sweden)

    Otakar Horák

    2013-06-01

    Full Text Available This paper begins by referring to the research which distinguishes two different aspects of subjective well-being, namely 1. experiences of happiness – measured by frequency and intensity of joy, stress, anger, worry and sadness we experience at a certain moment and 2. life satisfaction. Survey shows that beyond an annual household income of $75 000 there is no increase in the experiences of happiness. The paper then refers to the psychological mechanisms and factors – namely hedonic adaptation, social comparison and stress as a result of a raise – that lower the capacity of money to buy us the experiences of happiness. Not only we get quickly used to the things we buy, money also puts us into stressful situations and makes us build barriers between people. The paper then specifies strategies of economic decision-making that contribute to the maximization of happiness, such as purchase of the experiences instead of the things, interrupted and postponed consumption, or prosocial spending. The research indicates that prosocial spending has higher causal impact on promoting happiness than personal spending. As a matter of fact, this phenomenon is not culturally limited to the West. Concluding assertion – expressed only in the form of hypothesis – says that prosocial spending which is a form of prosocial behavior strengthens – through production of happiness – mutual relations in the society (cooperation.

  18. Ekonomika inovácie ako súčasť zvyšovania výkonnosti firmy

    Directory of Open Access Journals (Sweden)

    Chromjaková Felicita

    2009-12-01

    Full Text Available Základom dobre nastavenej inovácie je jej správna ekonomika, teda znalosť prietoku hodnoty, participujúcej v rôznych etapách na tvorbe inovácií a komplexnom inovačnom procese firmy. Prietok hodnoty má zásadný význam pre ekonomiku inovácie v tom, že na základe optimálne definovaných inovačných metrík dokáže identifikovať reálne ekonomické vstupy a výstupy a priamo poukázať na inovačný potenciál firmy a jeho úspešnosť z hľadiska zvyšovania budúcej výkonnosti firmy.The basis of a well balanced innovation is its right economy, that is so the knowledge of the value throughput, participating in the various phases of creating the innovation and of a complex innovation process of the company. Value throughput has an essential importance for the innovation economy there, where on the basis of optimally defined innovation metrics can be identified real economical inputs and outputs and directly the innovation potential of the ompany and its success from the point of view of the increase of its future efficiency.

  19. Ekonomický a politický vplyv Brazílie vo svetovej ekonomike ako súčasť BRICS

    OpenAIRE

    2012-01-01

    Countries Brazil, Russia, India, China and South Africa represent under the acronym BRICS a group of rapidly expanding and for the future very promising world economies. Their growing influence on a wide range of areas increases their potential to become major global players in the near future. The main objective of this bachelor thesis is according to the recent development of the global economy outlined in the first chapter and the analysis of current economic and political position of BRIC...

  20. Smiech ako moc a premeny človeka súčasnosti (The Laughter as a Power and Changes of Contemporary Human

    Directory of Open Access Journals (Sweden)

    Romana Javorčeková

    2016-03-01

    Full Text Available This article deals with the power of laugh as an expression of a human evoked by emotional, cognitive, physiological, and expressive changes which cause further changes in another person and society. Ambivalence is one of the basic characteristics of laugh which is why it cannot be fullly grasped in theory, even though it has been proved that joy expressed by laugh is the most universal emotion. Examples from movies serve well to observe laugh as a form of social gesture in contemporary society.

  1. Sociálny kapitál ako prevencia negatívnej agresivity adolescentov (Social Capital as a Prevention of Adolescents’ Aggressive Behaviours

    Directory of Open Access Journals (Sweden)

    Andrea Božeková

    2014-12-01

    Full Text Available Aggression as undesirable risk behaviour is a serious social problem which requires effective prevention and intervention solutions. This paper focuses on concept of Social Capital as an important factor in reduction of aggressive behaviour in family, school and neighbourhood. Relationships amongst family members, the involvement of parents in school activities, the participation of young people at school events and hobby groups and the activitiesof local youth and religious institutions can be identified as relevant components of social capital decreasing juvenile aggression. Positive social relationships offer an adequate prevention of adolescent aggression but also facilitate the inclusion of young people (who during adolescence and “dramatic clashes” create their personal identity, ultimatelydetermining their life as adults into society.

  2. Methanisation of stackable biomass in discontinuously operated solis fermentation plants; Methanisierung stapelbarer Biomassen in diskontinuierlich betriebenen Feststofffermentationsanlagen

    Energy Technology Data Exchange (ETDEWEB)

    Kusch, S.

    2007-07-01

    In contrary to the municipal waste processing in agricultural areas the biogas production based on solid materials is still in a phase of development. A possible processing approach is the discontinuously operated box fermenter with irrigation. Based on associated features like robustness, flexibility and utilizability in agricultural farms without liquid manure this type of fermenter is quite attractive.The author describes the laboratory and field tests and discusses possible applications, including procedural boundary conditions using different materials and problematic aspects. Possibilities for process optimizations are given.

  3. Segmentácia trhu pri použití marketingových dát ako súčasť marketingovej stratégie

    OpenAIRE

    Nagyová-Tkáčová, Monika

    2013-01-01

    The thesis deals mainly with market segmentation which is very important part of marketing strategy. The theoretical part consists of general knowledge of marketing, a Customer relationship management (CRM), market segmentation and database marketing. A case study consists of the implementation of the theoretical knowledge into the practical part. The aims of the thesis are: an introduction to the issue of market segmentation, an importance of market segmentation in creating marketing strateg...

  4. Zakotvená teória ako východisko pri hľadaní učiteľových stratégií k udržaniu svojej roly v triede

    Directory of Open Access Journals (Sweden)

    Csaba Igaz

    2013-10-01

    Full Text Available Článok sa zaoberá možnosťami využitia zakotvenej teórie pri kvalitatívnej analýze vyučovacích hodín s cieľom identifikovať rolu učiteľa a stratégie, ktoré umožňujú udržanie tejto roly. Takáto analýza sa môže využiť pri zavádzaní nových metód a prostriedkov do vyučovacieho procesu, kedy charakteristika rozdielov v správaní sa učiteľa počas bežnej vyučovacej hodiny a hodiny, v ktorej sa uplatňujú nové metódy nám umožní odhaliť ich slabé stránky. V príspevku sa podrobne venujeme postupu analýzy vyučovacích hodín. Na konkrétnych príkladoch vysvetlíme nielen aplikáciu otvoreného, axiálneho a selektívneho kódovania ale aj tzv. prechodnej úrovne kódovania, ktorá predstavuje nový prvok v zakotvenej teórií.

  5. Henry Kissinger a vietnamská vojna : myšlienky Henryho Kissingera ako akademika a praktická aplikácia z pozície štátnika

    OpenAIRE

    Garbarčík, Marek

    2009-01-01

    The main objective of this thesis is to clarify the background of steps, taken by Henry Kissinger during the Vietnam War in time, when he occupied position of the National Security Advisor of the United States of America. The second objective is to point at the position and evolution of the Vietnam War in US foreign policy context. The first part deals with analysis of Kissinger's academic papers, thoughts and approaches. The second part focuses on the description of US presidents aprroaches ...

  6. Pamäť ako múza: alétheia medzi enthusiasmom a epistémé (Memory as Muse: Alētheia between enthousiasm and epistēmē

    Directory of Open Access Journals (Sweden)

    Zuzana Zelinová

    2015-09-01

    Full Text Available The contribution underlines the role of Mnēmosynē (Memory as the mother of the Muses as a constitutive aspect of archaic poetry based on the etymological relationship between alētheia (as true knowledge and lēthē (as forgetting, in which truth becomes – in a specific way – the negation of forgetting. The social role of the poet, who participates in perpetuation of truth, is closely related to the complementarity produced by Muse and Memory. The main purpose of this paper is to offer an answer to the question: “How can be this relationship transformed from the area of poiētikē technē to the area of philosophy and what is the place of Mnēmosynē in Plato´s anamnēsis (dialogue Meno?”

  7. FIRST SYNOPTIC MAPS OF PHOTOSPHERIC VECTOR MAGNETIC FIELD FROM SOLIS/VSM: NON-RADIAL MAGNETIC FIELDS AND HEMISPHERIC PATTERN OF HELICITY

    Energy Technology Data Exchange (ETDEWEB)

    Gosain, S.; Pevtsov, A. A. [National Solar Observatory, 950 North Cherry Avenue, Tucson, AZ 85719 (United States); Rudenko, G. V.; Anfinogentov, S. A. [Institute of Solar-Terrestrial Physics (ISTP), Russian Academy of Sciences, Irkutsk (Russian Federation)

    2013-07-20

    We use daily full-disk vector magnetograms from Vector Spectromagnetograph on Synoptic Optical Long-term Investigations of the Sun system to synthesize the first Carrington maps of the photospheric vector magnetic field. We describe these maps and make a comparison of the observed radial field with the radial field estimate from line-of-sight magnetograms. Furthermore, we employ these maps to study the hemispheric pattern of current helicity density, H{sub c} , during the rising phase of solar cycle 24. The longitudinal average over the 23 consecutive solar rotations shows a clear signature of the hemispheric helicity rule, i.e., H{sub c} is predominantly negative in the north and positive in the south. Although our data include the early phase of cycle 24, there appears to be no evidence for a possible (systematic) reversal of the hemispheric helicity rule at the beginning of the cycle as predicted by some dynamo models. Furthermore, we compute the hemispheric pattern in active region latitudes (-30 Degree-Sign {<=} {theta} {<=} 30 Degree-Sign ) separately for weak (100 G < |B{sub r} | < 500 G) and strong (|B{sub r} | > 1000 G) radial magnetic fields. We find that while the current helicity of strong fields follows the well-known hemispheric rule (i.e., {theta} {center_dot} H{sub c} < 0), H{sub c} of weak fields exhibits an inverse hemispheric behavior (i.e., {theta} {center_dot} H{sub c} > 0), albeit with large statistical scatter. We discuss two plausible scenarios to explain the opposite hemispheric trend of helicity in weak and strong field regions.

  8. Operant learning and differential-reinforcement-of-low-rate 36-s responding in 5-HT1A and 5-HT1B receptor knockout mice.

    NARCIS (Netherlands)

    Pattij, T.; Broersen, L.M.; Linde, J. van der; Groenink, L.; Gugten, J. van der; Maes, R.A.A.; Olivier, B.

    2003-01-01

    Previous studies with mice lacking 5-HT(1A) (1AKO) and 5-HT(1B) (1BKO) receptors in hippocampus-dependent learning and memory paradigms, suggest that these receptors play an important role in learning and memory, although their precise role is unclear. In the present study, 1AKO and 1BKO mice were s

  9. Applci ation of TOPSIS Based on Entropy Weight in Soli Qualti y Assessment of Songhua River Area%基于熵权的TOPSIS模型在松花江地区土壤评价应用

    Institute of Scientific and Technical Information of China (English)

    张春宇

    2015-01-01

    Soil is the material of agriculture base , therefore , preservation and improvement of soil quality is the basic task for agricultural sustainable development.Based on Songhua river area as an example , this paper refered other documents and combined the actual situation of the study area to grade , attempt to using TOPSIS model from practicality and versatility of soil quality are analyzed.From the results can be clearly seen the region′s soil types of good or bad sort , for future scholars can provide powerful evidence.%以松花江地区为例,参考已有文献,选取指标和等级划分标准,利用TOPSIS模型从实用性、多功能性对土壤质量进行分析。从得的到的结果中可以很清晰看出各种土质量由好到坏的排序,并为今后土壤的综合评价提供有力依据。

  10. Disease: H00438 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available Nasu and review. Neuropathology (2010) PMID:20836191 (gene, description) Bianchin MM, Martin KC, de Souza A...ko M, Sano K, Nakayama J, Amano N Nasu-Hakola disease: The first case reported by

  11. Interferencije svojstvene kvantitativnim imunokemijskim metodama

    OpenAIRE

    2009-01-01

    Antitijelo koje se u imunokemijskim metodama koristi kao reagens otkriva ciljni analit (antigen). Iako je nekovalentna veza između analita i komplementarnog antitijela specifična, moguće su lažno pozitivne ili negativne interferencije. Neke su interferencije slične interferencijama kod kemijskih analiza, a neke su svojstvene samo imunokemijskim analizama. Na interferencije u imunokemijskim metodama treba pomisliti ako se dobije neprihvatljiv rezultat, ako postoji nelinearnost prilikom razrjeđ...

  12. 75 FR 61577 - Wage Methodology for the Temporary Non-Agricultural Employment H-2B Program

    Science.gov (United States)

    2010-10-05

    ... normal business hours at the Employment and Training Administration (ETA) Office of Policy Development... Agricolas (CATA) v. Solis, Civil No. 2:09-cv-240-LP, 2010 WL 3431761 (E.D. Pa. Aug. 30, 2010), ordered the... a los Trabajadores Agricolas (CATA) v. Solis, 2010 WL 3431761, at *19 n.22, which invalidated...

  13. Long- and Short-Term Processes Indicated by the Displacement of the Chamber Roofs in the Monumental Wieliczka Salt Mine / Długo- i krótkotrwałe procesy sygnalizowane przemieszczeniami stropu komór w zabytkowej kopalni soli wieliczka

    Science.gov (United States)

    Kortas, Grzegorz

    2013-03-01

    This paper presents several processes that are characteristic for the salt rock mass, which have been found upon analysis of observations of the chamber roof displacements in the monumental Wieliczka Salt Mine. Those processes are revealed in different time frames and they range from short- to long-term ones. A regular long-term increase or decrease of creeping rate in the chamber roof is expressed by power function of time. It was found that an annual component of that process consists in the season cycle, marked by increased displacement rates in the summer and reduced ones in the winter. The seasonal changes in air humidity are the cause of those cycles. A rock slip cycle is observed in some chambers during several months. In those periods, we can identify displacements that happen when internal static and dynamic friction is overcome. In 2007-2009, single signals from large-area rock-mass movements were recorded in several chambers. They were probably associated with the change of geo-mechanical conditions in northern region of rock mass after a leak had been plugged in the cross-corridor Mina. The results of our analysis have revealed new cognitive components contributing to the description of rock behaviour in specific geological and mining conditions of Miocene salt deposits.

  14. Ühepäevaliblika lend üle valgete laikude / Sven Karja

    Index Scriptorium Estoniae

    Karja, Sven, 1968-

    2000-01-01

    Teatrikriitika olukorrast - kiirtempo, esietenduse põhjal arvustamine, väikelinnateatrite keskmised lavastused jäävad kajastuseta. Vaadeldakse hooaja kolme "mahavaikitud " lavastust - Akos Kertészi "Lesed" Eili Neuhausi lavastuses Rakvere Teatris, Mario Fratti "Ohver" Madis Kalmeti lavastuses Pärnu Endlas ja David Williamsoni "Sõbrad ja raha" Viljandi Ugalas.

  15. GABA(A)-benzodiazepine receptor complex sensitivity in 5-HT(1A) receptor knockout mice on a 129/Sv background.

    NARCIS (Netherlands)

    Pattij, T.; Groenink, L.; Oosting, R.S.; Gugten, J. van der; Maes, R.A.A.; Olivier, B.

    2002-01-01

    Previous studies in 5-HT(1A) receptor knockout (1AKO) mice on a mixed Swiss Websterx129/Sv (SWx129/Sv) and a pure 129/Sv genetic background suggest a differential gamma-aminobutyric acid (GABA(A))-benzodiazepine receptor complex sensitivity in both strains, independent from the anxious phenotype. To

  16. Professional School Counseling Evaluation Rubric: Advocating for the Profession through Awareness and Accountability

    Science.gov (United States)

    Morris, Carrie A. Wachter; Slaten, Christopher D.

    2014-01-01

    Professional school counselors have been advocating for their role as counselors in the schools for decades (Galassi & Akos, 2007; Gysbers, 2002; Slaten & Baskin, 2013). Although researchers have addressed this concern through advocacy in service and writing, school counselors continue to perform a significant amount of non-counseling…

  17. The Promotion of Teaching Excellence in Higher Education: A Comparison of the Australian and New Zealand Approaches

    Science.gov (United States)

    Abbott, Malcolm

    2012-01-01

    In this paper a comparison is made between the structure and operations of the Australian Learning and Teaching Council and the Ako Aotearoa National Centre for Tertiary Teaching Excellence of New Zealand. Both of these organisations were established in the mid 2000s and were created at a time when higher education institutions in both countries…

  18. School Counselors' Attitudes towards Providing Services to Students Receiving Section 504 Classroom Accommodations: Implications for School Counselor Educators

    Science.gov (United States)

    Romano, Dawn M.; Paradise, Louis V.; Green, Eric J.

    2009-01-01

    Questions have arisen regarding counselor's capabilities in assisting students with special needs (Milsom & Akos, 2003; Studer & Quigney, 2005). This study examined school counselors' training and attitudes toward providing services to students with learning disabilities who qualified for services only under Section 504 of the Rehabilitation Act.…

  19. 3Corners of the World / Joosep Sang

    Index Scriptorium Estoniae

    Sang, Joosep

    2009-01-01

    Arvustused : Robert Jürjendal, Petri Kuljuntausta, David Rothenberg. 3Corners of the World. [Tallinn] : Terra Nova Music, 2008. Nadia ja Fragile. Uhke ali. [Tallinn] : Nadia & Fragile, 2008. UMA. Civitas Soli. [Tallinn] : Elwood, 2008. Uutest heliplaatidest

  20. Estonian science breeds world-class researches / ToivoTänavsuu, Hille Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Toivo

    2010-01-01

    Eesti teadusfirmadest: biotehnoloogia ettevõtted Celecure AS, Protobios OÜ, Icosagen AS, Asper Biotech AS ja Solis BioDyne OÜ, geenitehnoloogia firma Genorama OÜ, keemiatehnoloogia ettevõte AS Cambrex Tallinn

  1. 78 FR 44054 - Wage Methodology for the Temporary Non-Agricultural Employment H-2B Program; Proposed Delay of...

    Science.gov (United States)

    2013-07-23

    ... business hours at the Employment and Training Administration (ETA) Office of Policy Development and... Agricolas (CATA) v. Solis, -- F. Supp. 2d --, 2013 WL 1163426 (E.D. Pa. 2013) in which the district...

  2. 77 FR 10037 - Temporary Non-Agricultural Employment of H-2B Aliens in the United States

    Science.gov (United States)

    2012-02-21

    ... Trabajadores Agricolas (CATA) v. Solis, Civil No. 2:09-cv-240-LP, 2010 WL 3431761 (E.D. Pa. Aug. 30, 2010..., including small business employers, U.S. and H-2B workers, worker advocacy groups, State Workforce...

  3. 77 FR 2347 - Additional Designations, Foreign Narcotics Kingpin Designation Act

    Science.gov (United States)

    2012-01-17

    ... blocked pursuant to section 805(b) of the Kingpin Act. 1. ALVAREZ ZEPEDA, Oscar, Avenida Francisco Solis... Universitarios No. 789, Local 4, Colonia Villa Universidad, Culiacan, Sinaloa C.P. 80010, Mexico; Localidad...

  4. JPRS Report, East Asia Southeast Asia

    Science.gov (United States)

    2007-11-02

    these limitations as a challenge. Protacio also congratulated the Second Air Division under Commander Brig. Gen. Leopoldo Acot Jr. for its assistance...his sincerity in achieving cultural harmony and soli - darity. Thirdly, they demanded that he be more consultative on matters affecting them, to...Chongsutthamani, the brother of Mr. Mongkhon Chongsutthamani, a Soli - darity Party MP from Chiang Rai Province who serves as the secretary to Mr

  5. Considerations of Pharmacology on Fitness for Duty in the Operational Environment

    Science.gov (United States)

    2007-05-01

    and Performance Division Biobehavior, Bioassessment & Biosurveillance Branch Brooks City-Base, TX 9. SPONSORING / MONITORING AGENCY NAME...S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) Air Force Materiel Command Biobehavior, Bioassessment & Biosurveillance Branch 711 HPW/RHP 711...survey of all (active duty and deployed) Army aviators using the Army Knowl- edge Online (AKO) portal to ask individuals to anony- mously respond to

  6. Reverzibilna transformacija plastida u panaširanim listovima vrste Euonymus fortunei var. radicans

    OpenAIRE

    Antica, Mariastefania; Wrischer, Mercedes

    1982-01-01

    Istraženi su ultrastruktura, sadržaj pigmenata i fotosintetska aktivnost plastida panaširanih listova vrste Euonymus fortunei var. radicans. Normalni kloroplasti središnjih tamnozelenih dijelova lista imaju velika grana, mnogo klorofila i visoku fotosintetsku aktivnost. Rubni dijelovi lista, koji sadrže mutirane plastide, vrlo su osjetljivi na osvjetljenje, te mijenjaju boju ovisno o intenzitetu svjetlosti. Ako rastu u sjeni, oni su svjetlozeleni i sadrže kloroplaste s malo grana. Na jakoj...

  7. Army Leader Transitions Handbook

    Science.gov (United States)

    2008-01-01

    to improving the organization. The following are some effective methods and a compilation of techniques and considerations which have been used... improve your understanding of the environment. Outgoing Leader’s Assessment An important item to obtain from the current leader is a list of key contacts...The courseware allows users to tailor training materials to specific needs. Users access this at the CAL AKO website or Army eLearning https

  8. Phenolic compounds variation in Mentha L. Species in the course of a four-years period / Kolísanie fenolových látok v rôznych druhoch Mentha L. Počas 4 ročného obdobia

    Directory of Open Access Journals (Sweden)

    Fialová S.

    2015-06-01

    Full Text Available Mäty patria medzi významné rastliny čeľade Lamiaceae. Okrem silice obsahujú množstvo cenných fenolových látok, vrátane flavonoidov a fenolových kyselín, ktoré sa spolupodieľajú na farmakologickej aktivite mäty. V našej práci sme hodnotili kolísanie obsahu fenolových látok počas 4 rokov vegetácie. Pri 1- až 4-ročných rastlinách sme porovnali v suchých listoch obsah hydroxyškoricových derivátov vyjadrených ako kyselina rozmarínová (λ = 505 nm, polyfenolov a trieslovín vyjadrených ako kyselina rozmarínová (λ = 760 nm a flavonoidov vyjadrených ako luteolín-7-O-glukozid (λ = 392 nm a kvercetín (λ = 420 nm. Využili sme spektrofotometrické metódy Európskeho liekopisu. Naše výsledky poukazujú na vysoké hodnoty fenolových látok najmä v 3- a 4-ročných porastoch.

  9. New Initiatives for Synoptic Observations

    Indian Academy of Sciences (India)

    C. U. Keller

    2000-09-01

    Several new synoptic facilities for long-term studies of the Sun will become operational within the next few years. This paper summarizes information on some of these projects, in particular GONG+, ISOON, GOES/SXI, and SOLIS. SOLIS, the Synoptic Optical Long-Term Investigations of the Sun, is currently being built by the National Solar Observatory and will become operational in 2001. It consists of a 50-cm vector spectromagnetograph, a 14-cm full-disk patrol, and an 8-mm sunas-a-star spectrometer.

  10. Adsorpcija pesticida na zeolitima, polianilinu i njihovim kompozitima

    OpenAIRE

    Milojević-Rakić, Maja

    2014-01-01

    Katalitička svojstva Fe-modifikovanih zeolita zavise od formiranja dimernih i oligomernih vrsta gvožđa, kao i čestica gvožđe-oksida. U ovoj doktorskoj disertaciji je sinteza Femodifikovanih ZSM-5 zeolita izvedena metodom jonske izmene iz razblaženih rastvora različitih soli (Fe(III)citrata, Fe(II)oksalata i Fe(III)nitrata). Pokazano je da aktivnost Femodifikovanih zeolita kao katalizatora zavisi od soli gvožđa u čijem prisustvu se vrši izmena, što ukazuje da je moguće, iz razbl...

  11. Genetics Home Reference: Kaufman oculocerebrofacial syndrome

    Science.gov (United States)

    ... 1 link) PubMed OMIM (1 link) KAUFMAN OCULOCEREBROFACIAL SYNDROME Sources for This Page Basel-Vanagaite L, Dallapiccola B, Ramirez-Solis R, Segref A, Thiele H, Edwards A, Arends MJ, Miró X, White JK, Désir ...

  12. Brasil, estudos pós-coloniais e contracorrentes análogas: entrevista com Ella Shohat e Robert Stam

    NARCIS (Netherlands)

    dos Santos, E.R.S.; Schor, P.

    2013-01-01

    Interview with Ella Shohat and Robert Stam (Portuguese translation). Original version: Brazil is not Traveling Enough”: On Postcolonial Theory and Analogous Counter-Currents (http://www2.let.uu.nl/solis/psc/P/PVOLUMEFOUR/PVOLUMEFOURPAPERS/P4SHOHAT-STAM-INTERVIEW.pdf)

  13. Porous structure and particle size of silica and hydrotalcite catalyst precursors

    NARCIS (Netherlands)

    Titulaer, M.K.

    1993-01-01

    The subject of this thesis is the control of the porous structure of catalyst bodies. The first part deals with silica, that can be utilized as catalyst support with many industrially important catalytic reactions. The second part of the thesis deals with the preparation and characterization of soli

  14. Towards Reengineering the United States Department of Defense: A Financial Case for a Functionally-Aligned, Unified Military Structure

    Science.gov (United States)

    2014-03-01

    identify actual costs of DoD functions. v DEDICATION Soli Deo Gloria To my beautiful ...must develop an activity dictionary identifying the activities it performs. An activity is a verb and an associated object, i.e. fly aircraft. This

  15. DMPD: Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and controlof anti-tumor activity in primary macrophages. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 16846591 Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and controlof anti...Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and controlof anti... IFN-alpha gene regulation and controlof anti-tumor activity in primary macrophages. Authors Solis M, Goubau

  16. 78 FR 24047 - Wage Methodology for the Temporary Non-Agricultural Employment H-2B Program, Part 2

    Science.gov (United States)

    2013-04-24

    ... Comit de Apoyo a los Trabajadores Agricolas v. Solis, which vacated portions of DOL's current prevailing... Department receives available for public inspection during normal business hours at the Employment and...: Kevin J. Cummings, Chief, Business and Foreign Workers Division, Office of Policy and Strategy,...

  17. 76 FR 37686 - Wage Methodology for the Temporary Non-Agricultural Employment H-2B Program; Amendment of...

    Science.gov (United States)

    2011-06-28

    ... Comit de Apoyo a los Trabajadores Agricolas (CATA) v. Solis, Civil No. 2:09-cv-240-LP (E.D. Pa.), the... also make all the comments it receives available for public inspection during normal business hours at... Department could not phase in a wage regime based upon a desire to alleviate hardship on small...

  18. 76 FR 15129 - Temporary Non-Agricultural Employment of H-2B Aliens in the United States

    Science.gov (United States)

    2011-03-18

    ... it receives available for public inspection during normal business hours at the Employment and... the Eastern District of Pennsylvania in Comit de Apoyo a los Trabajadores Agricolas (CATA) v. Solis... representatives from business, labor and other stakeholders, as part of its annual strategic planning...

  19. The Datsuns : külalised eriti kaugelt. Popid poisid Norrast

    Index Scriptorium Estoniae

    2007-01-01

    Uus-Meremaa rockansamblist The Datsuns (www.thedatsuns.com), albumitest "The Datsuns", "Smoke and Mirrors". Norra rockansamblist Apoptygma Berzerk (www.apoptygmaberzerk.de), albumitest "Soli Deo Gloria, "7", "Welcome To Earth", "Harmonizer", "You and Me Against the World", "Sonic Diary". Info festivalist: www.rabarock.delfi.ee / www.rabarock.ee

  20. Direct numerical simulation of three-dimensional coherent structure in plane mixing layer

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The three-dimensional temporally evolving plane mixing layer is sinulated by directly solying the Navier-Stokes equations using pseudo-spectral method. The process of loss of stability, and the formation paring, and development of vortex are presented. The simulated result shows that the evolving characteristics of coherent structure are important mechanism of growing and entrainment of mixing layer.

  1. Inherent hazards, poor reporting and limited learning in the solid biomass energy sector: A case study of a wheel loader igniting wood dust, leading to fatal explosion at wood pellet manufacturer

    DEFF Research Database (Denmark)

    Hedlund, Frank Huess; Astad, John; Nichols, Jeffrey

    2014-01-01

    Large loaders are commonly used when handling solid biomass fuels. A preventable accident took place in 2010, where the malfunction of a front-end wheel loader led to a dust explosion which killed the driver and destroyed the building. The case offers an opportunity to examine the hazards of soli...

  2. Crude Oil Stockpiling Needed in China

    Institute of Scientific and Technical Information of China (English)

    Fu Bin

    1997-01-01

    @@ The two oil crises in the 1970's had badly disturbed the economy and the way of life of those oil importing countries, which began to realize the importance of oil stockpiling. With the rapid development of economy, China could no longer satisfy itself soly with the domesticallyproduced oil.

  3. Measurements of solids concentration and axial solids velocity in gas-solid two-phase flows.

    NARCIS (Netherlands)

    Nieuwland, J.J.; Meijer, R.; Kuipers, J.A.M.; Swaaij, van W.P.M.

    1996-01-01

    Several techniques reported in the literature for measuring solids concentration and solids velocity in (dense) gas-solid two-phase flow have been briefly reviewed. An optical measuring system, based on detection of light reflected by the suspended particles, has been developed to measure local soli

  4. Effective poroelastic model for one-dimensional wave propagation

    NARCIS (Netherlands)

    Kudarova, A.; Van Dalen, K.N.; Drijkoningen, G.G.

    2013-01-01

    An effective poroelastic model is proposed that describes seismic attenuation and dispersion in periodically layeredmedia. In this model, the layers represent mesoscopic-scale heterogeneities (larger than the grain and pore sizes but smaller than the wavelength) that can occur both in fluid and soli

  5. Maleic acid treatment of bioabated corn stover liquors improves cellulose conversion to ethanol

    Science.gov (United States)

    Elimination of inhibitory compounds released during pretreatment of lignocellulose is critical for efficient cellulose conversion and ethanol fermentation. This study examined the effect of bioabated liquor from pretreated corn stover on enzyme hydrolysis of Solka Floc or pretreated corn stover soli...

  6. Building and Verifying a Predictive Model of Interruption Resumption

    Science.gov (United States)

    2012-03-01

    contain gist information. Because these episodic codes decay, the current one will always be the most active ( modulo effects of activation noise), so...Li, A. Blandford, P. Cairns, and R. M. Young , BThe effect of interruptions on postcompletion and other procedural errors: An account based on the

  7. Eestlased rajavad omaenda oaasi Ränioru mägismaal / Martin Hanson

    Index Scriptorium Estoniae

    Hanson, Martin, 1984-

    2008-01-01

    EAS-i California esinduse töötaja Andrus Viirg tutvustab Eesti ettevõtete võimalusi äritegevuse alustamiseks ja investorite leidmiseks Silicon Valley's. Vt. samas: Skype'i tuules lendavad ettevõtted. San Joses kavandavad kontori avamist Eesti ettevõtted Yoga Intelligence, Myoton ja Solis Biodyne

  8. Genetic Factors in Breast Cancer: Center for Interdisciplinary Biobehavioral Research

    Science.gov (United States)

    2008-10-01

    Cancer Institute of New Jersey); Erin Moshier, Elizabeth Zaita, Katie Dorn, Anneisha Camp- bell, Ana Estevez, Joan Bratton, Melissa Solis, Sherly Jacob...18) Costa -Pinto FA, Palermo-Neto J: Neuroimmune interactions in stress, Neuroimmunomodulation 2010; 17(3):196-9. 19) Smith AJP, Humphries SE

  9. Combining two technologies for full genome sequencing of human.

    Science.gov (United States)

    Skryabin, K G; Prokhortchouk, E B; Mazur, A M; Boulygina, E S; Tsygankova, S V; Nedoluzhko, A V; Rastorguev, S M; Matveev, V B; Chekanov, N N; D A, Goranskaya; Teslyuk, A B; Gruzdeva, N M; Velikhov, V E; Zaridze, D G; Kovalchuk, M V

    2009-10-01

    At present, the new technologies of DNA sequencing are rapidly developing allowing quick and efficient characterisation of organisms at the level of the genome structure. In this study, the whole genome sequencing of a human (Russian man) was performed using two technologies currently present on the market - Sequencing by Oligonucleotide Ligation and Detection (SOLiD™) (Applied Biosystems) and sequencing technologies of molecular clusters using fluorescently labeled precursors (Illumina). The total number of generated data resulted in 108.3 billion base pairs (60.2 billion from Illumina technology and 48.1 billion from SOLiD technology). Statistics performed on reads generated by GAII and SOLiD showed that they covered 75% and 96% of the genome respectively. Short polymorphic regions were detected with comparable accuracy however, the absolute amount of them revealed by SOLiD was several times less than by GAII. Optimal algorithm for using the latest methods of sequencing was established for the analysis of individual human genomes. The study is the first Russian effort towards whole human genome sequencing.

  10. Synthesis of novel photochromic pyrans via palladium-mediated reactions

    NARCIS (Netherlands)

    Böttcher, Christoph; Zeyat, Gehad; Ahmed, Saleh A.; Irran, Elisabeth; Cordes, Thorben; Elsner, Cord; Zinth, Wolfgang; Rueck-Braun, Karola

    2009-01-01

    Photochromic pyrans for applications in material and life sciences were synthesized via palladium-mediated cyanation, carbonylation and Sonogashira cross-coupling starting from a bromo-substituted naphthopyran and benzopyrans. A novel photoswitchable benzopyran-based ω-amino acid for Fmoc-based soli

  11. 75 FR 79803 - Semiannual Agenda of Regulations

    Science.gov (United States)

    2010-12-20

    ... some kinds of flavorings, foods, and beverages. They have begun to use substitutes such as 2,3... the Department's agenda. Hilda L. Solis, Secretary of Labor. Office of Labor-Management Standards...-Management Standards--Completed Actions Regulation Sequence Title Identifier Number Number 397...

  12. GLASS-FIBER SELF-MIXING DIODE-LASER DOPPLER-VELOCIMETER

    NARCIS (Netherlands)

    KOELINK, MH; SLOT, M; DEMUL, FFM; GREVE, J; GRAAFF, R; DASSEL, ACM; AARNOUDSE, JG

    1992-01-01

    A novel diode-laser Doppler velocimeter based on self-mixing feedback of Doppler-shifted light in the laser through a single glass fibre is described. The instrument can be applied, for example, to the invasive measurement of blood velocities in blood vessels, and of other industrial fluids and soli

  13. Minimaal kinderopvang

    NARCIS (Netherlands)

    S.G. van der Lecq (Fieke)

    2001-01-01

    textabstractWie dacht dat Amerikanen alleen maar hard en lang werken, loopt achter: de balans tussen werk en privé heeft nu de kolommen van de Harvard Business Review gehaald 1. Ook in de VS speelt de discussie tussen werkende ouders en hun kinderloze collega's over de grenzen van de onderlinge soli

  14. Unpacking Cyberwar: The Sufficiency of the Law of Armed Conflict in the Cyber Domain

    Science.gov (United States)

    2013-01-01

    Judg- ment of June 27, 1986, LEXIS 4. 9 Paul Walker, “Assessing Actions Along the Spectrum of Cyberspace Operations,” PowerPoint briefing at the...Naval War College, 2011). 13 See Nicaragua v. United States; also Oil Plat- forms Case (2003 ICJ LEXIS 11). 14 Solis, 149. 15 Ibid., 167–168. 16

  15. Genetic deletion of NR3A accelerates glutamatergic synapse maturation.

    Directory of Open Access Journals (Sweden)

    Maile A Henson

    Full Text Available Glutamatergic synapse maturation is critically dependent upon activation of NMDA-type glutamate receptors (NMDARs; however, the contributions of NR3A subunit-containing NMDARs to this process have only begun to be considered. Here we characterized the expression of NR3A in the developing mouse forebrain and examined the consequences of NR3A deletion on excitatory synapse maturation. We found that NR3A is expressed in many subcellular compartments, and during early development, NR3A subunits are particularly concentrated in the postsynaptic density (PSD. NR3A levels dramatically decline with age and are no longer enriched at PSDs in juveniles and adults. Genetic deletion of NR3A accelerates glutamatergic synaptic transmission, as measured by AMPAR-mediated postsynaptic currents recorded in hippocampal CA1. Consistent with the functional observations, we observed that the deletion of NR3A accelerated the expression of the glutamate receptor subunits NR1, NR2A, and GluR1 in the PSD in postnatal day (P 8 mice. These data support the idea that glutamate receptors concentrate at synapses earlier in NR3A-knockout (NR3A-KO mice. The precocious maturation of both AMPAR function and glutamate receptor expression are transient in NR3A-KO mice, as AMPAR currents and glutamate receptor protein levels are similar in NR3A-KO and wildtype mice by P16, an age when endogenous NR3A levels are normally declining. Taken together, our data support a model whereby NR3A negatively regulates the developmental stabilization of glutamate receptors involved in excitatory neurotransmission, synaptogenesis, and spine growth.

  16. DEFINIRANJE PODRUČJA I POTENCIJAL RAZVOJA SOCIJALNOG PODUZETNIŠTVA HRVATSKE U KOMPARATIVNOM KONTEKSTU

    OpenAIRE

    Vincetić, Vlatka; Babić, Zdenko; Baturina, Danijel

    2013-01-01

    Socijalno poduzetništvo i socijalna ekonomija u protekla se tri desetljeća kontinuirano razvijaju na globalnoj razini, kako u razvijenim zemljama, ako i u zemljama u razvoju. Socijalno poduzetništvo prestavlja kišobran termin za velik raspon inovativnih i dinamičnih internacionalnih praksi i diskursa u socijalnom i ekološkom području. Za neke je paradigma socijalnog poduzetništva panaceja rješavanju problema tržišnih neuspjeha, dok je za druge manifestacija dodatne ekspanzije i dominacije pos...

  17. A Multi-Purpose Astronaut Shower for Long-Duration Mirogravity Missions

    Science.gov (United States)

    Bernasconi, M. C.; Versteeg, M.; Zenger, R.

    An initial study for an Advanced Kind Of Shower (AKOS) has attempted to define the need and advantages, as well as develop a concept, for an astronaut's shower. Relying on past studies, on flight experiences, and on the ensuing critiques, and building on an interdisciplinary approach, the study defined a modular piece of equipment that - using parts inspired by commercially- available products - that for the first time could satisfy different functions for the hygiene, well-being, and health support of the crew members.

  18. Lack of Adipocyte AMPK Exacerbates Insulin Resistance and Hepatic Steatosis through Brown and Beige Adipose Tissue Function

    DEFF Research Database (Denmark)

    Mottillo, Emilio P; Desjardins, Eric M; Crane, Justin D

    2016-01-01

    Brown (BAT) and white (WAT) adipose tissues play distinct roles in maintaining whole-body energy homeostasis, and their dysfunction can contribute to non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. The AMP-activated protein kinase (AMPK) is a cellular energy sensor, but its role...... in mitochondrial structure, function, and markers of mitophagy. In response to a high-fat diet, iβ1β2AKO mice more rapidly developed liver steatosis as well as glucose and insulin intolerance. Thus, AMPK in adipocytes is vital for maintaining mitochondrial integrity, responding to pharmacological agents...

  19. Najčešće sintaktičke pogreške u publicističkome stilu (na primjeru "Glasa Istre")

    OpenAIRE

    2013-01-01

    U radu se opisuju najčešće sintaktičke pogreške u Glasu Istre, najčitanijim dnevnim novinama u Istri. Analiza obuhvaća dvadesetak brojeva s posebnim naglaskom na položaj enklitike, sročnost s brojevima, veznike budući da, ni i niti, s obzirom na (to da), ukoliko i ako, prijedloge nasuprot, unatoč, usprkos, kroz, po, putem, s(a), zbog i radi, posvojni genitiv, posvojni pridjev i dekompoziciju predikata. Uvažavajući pravila i posebnosti publicističkoga stila, komparativnom se analizom propisâ i...

  20. The Magnitude and Regional Distribution of Needs for Hydropower - Phase II Future Electric Power Supply and Demand. Volume 4

    Science.gov (United States)

    1981-09-01

    llliN015 COAPS Of INGINUaS HIL MACii’III’!"UOE AND REGIONAl OISTRi8UTION OF NEED JOR UYOROPOM:Il Til( NATIONAl ttYOfiOPOWt:R STUDY PROJECTIONS OF...AWO, IL UNO IS COaPS Of INGINIUl THE MAGNifUOl. AND REGIONAL lliSTHIBUTION Of NHO FOR HVCROPOWI:H Tttl:: NATIONAL tiVOROPOWlH STIJOY PROJECTIONS OF...RUOUICU CHICAGO, IlliNOIS coaPS Of INGINIIaJ lHl MAUNITI)(lE AkO REGIONAL OISTRI&UTION Of NEED FOR HVOROPOWt:U THE NATIONAL HVUAOPOWER STUIJV

  1. Sintaksa i semantika u suprotan koordinacije i zamjene

    Directory of Open Access Journals (Sweden)

    Pavao Tekavčić

    1978-12-01

    Full Text Available U onome što se obično naziva adverzativnim rečenicama valja razlikovati dva tipa: adverzativne rečenice (u užem smislu, kojima se izriče kontrast (Laura è bella ma è antipatica, i supstitutivne rečenice, kojima se prvi član ukida i zamjenjuje drugim (Laura non è bella bensì ( è brutta. U studiju ovih dvaju tipova rečenica mogu se razlikovati neke sintaktičke i semantičke kategorije: 1 identitet/kontrast, 2 djelomičnost/potpunost, S afirmacija/ negacija svakoga od dvaju članova, 4 pozitivno/negativno (semantičko značenje članova. U članku se studira semantička prihvatljivost rečenica, restrikcije u upotrebi triju glavnih veznika (però, bensì, ma i mogućnost elipse drugoga pomoćnog glagola. Ako prvi član (A nije negiran, moguć je jedino veznik u značenju 'ali', ali samo onda ako se radi o djelomičnom identitetu i djelomičnom kontrastu, dok je isključen ako se izražava potpuni identitet (*Laura è bella pero è bella ili potpuni kontrast (*Laura è bella pero è brutta. Veznik pero u današnjem talijanskom jeziku ima samo značenje 'ali' Ako je prvi član negiran, uz 'ali' moguce je i 'nego' (tal.bensì, ali s razlikom u značenju (Laura non è bella pero è simpatica. I Laura non è bella bensi è simpatica. Veznik 'nego' isključen je samo onda kada se izražava potpuni identitet (jer nema smisla zamjenjivati jedan pojam drugim, ako su identični, dok je u drugim slučajevima - uz veće ili manje razlike, i ev u posebnim slučajevima - prihvatljiv. Dok però znači samo 'ali' a bensì samo 'nego', treći veznik, ma, koji je danas najčešći, pokriva oba ta značenja. Stoga, ako je prvi član negiran, može doći do dvoznačnosti: Laura non è bella, ma è simpatica može značiti da L. nije lijepa nego simpatična (supstitutivna reč. ili da nije lijepa ali  je simpatična (adverzativna reč.. U takvim rečenicama elipsa drugoga pomoćnog glagola postaje distinktivna: dok će se rečenica Laura non è bella

  2. Cognitive Load Study Using Increasingly Immersive Levels of Map-based Information Portrayal on the End User Device

    Science.gov (United States)

    2012-09-01

    based games 4.69 Internet 5.38 AKO 4.06 My Pay 4.13 Smart phone 4.91 Army digital systems (e.g. FBCB2) 1.81 I would self rate my computer skills...monitoring it. 1 Letting objects rotate around me makes pathfinding tougher when referencing the map. 1 Knowing which building is which is...position relative to the route. A fixed perspective allows you to pathfind to and from your point of departure without known waypoints changing

  3. Geodetski časopisi u Emerging Sources Citation Indexu

    Directory of Open Access Journals (Sweden)

    Nedjeljko Frančula

    2016-06-01

    U ESCI su uvršteni časopisi od regionalne važnosti i časopisi iz znanstvenih područja u nastajanju. Časopisi uvršteni u ESCI bit će nakon određenog vremena, ako zadovolje kriterije Thomson Reutersa, uvršten u SCIE, SSCI ili A&HCI. Za časopise uvrštene u ESCI, trenutno ih je 2589, ne izračunava se faktor odjeka.

  4. Vyukový program pro demonstraci metod texturování 3D objektů

    OpenAIRE

    Kukla, Michal

    2008-01-01

    Práca sa zaoberá vývojom aplikácie pre demonštrovanie metód texturovania 3D objektov. Práca obsahuje teoreticky rozobrané jednotlivé metódy textúrovania, ktoré tvoria základ pre návrh obsahu demonštrácie. Požiadavky na aplikáciu sú analyzované a aplikované pre vytvorenie návrhu aplikácie. Postupy pri implementácii jednotlivých metód textúrovania a popis ovládacích prvkov aplikácie sú popísané v závere práce. Program môže slúžiť ako demonštračný  pri výučbe alebo tiež ako študijný materiá...

  5. A Far-Ultraviolet Spectroscopic Survey of the Globular Cluster 47 Tuc

    CERN Document Server

    Knigge, C; Zurek, D R; Long, K S; Gilliland, R L

    2000-01-01

    We present preliminary results from a FUV spectroscopic and photometric survey of the globular cluster 47 Tuc. Our main goal is to either confirm or rule out the existence of a large population of CVs in the core of this cluster. We have so far identified approximately 425 FUV sources, most of which are probably hot, young WDs. However, we have also found approximately 30 FUV sources whose position in a FUV-optical color-magnitude diagram makes them strong CV candidates. If most or all of these objects are eventually confirmed as CVs, the large CV population predicted by tidal capture theory has finally been found. Our data has also allowed us to resolve the long-standing puzzle surrounding AKO 9, a UV-bright and highly variable 1.1 day binary system in 47 Tuc. AKO 9 is the brightest FUV source in our data and presents a blue FUV spectrum with strong CIV and HeII emission lines. Its spectrum is similar to that of the long-period, DN-type field CV GK Per. Based on this similarity and other evidence, we suggest...

  6. Transcriptome of pancreas-specific Bmpr1a-deleted islets links to TPH1–5-HT axis

    Directory of Open Access Journals (Sweden)

    Fang-Xu Jiang

    2015-08-01

    Full Text Available Bone morphogenetic protein (BMP signaling is crucial for the development and function of numerous organs, but its role on the function of pancreatic islets is not completely clear. To explore this question, we applied the high throughput transcriptomic analyses on the islets isolated from mice with a pancreas-specific deletion of the gene, Bmpr1a, encoding the type 1a BMP receptor. Consistently, these pBmpr1aKO mice had impaired glucose homeostasis at 3 months, and were more severely affected at 12 months of age. These had lower fasting blood insulin concentrations, with reduced expression of several key regulators of β-cell function. Importantly, transcriptomic profiling of 3-month pBmpr1aKO islets and bioinformatic analyses revealed abnormal expression of 203 metabolic genes. Critically among these, the tryptophan hydroxylase 1 gene (Tph1, encoding the rate-limiting enzyme for the production of 5-hydroxytryptamine (5-HT was the highest over-expressed one. 5-HT is an important regulator of insulin secretion from β cells. Treatment with excess 5-HT inhibited this secretion. Thus our transcriptomic analysis links two highly conserved molecular pathways the BMP signaling and the TPH1–5-HT axis on glucose homeostasis.

  7. What are you waiting for?

    CERN Multimedia

    CERN video productions

    2012-01-01

    Video artists: Sandor Tokai, Gyorgy Ujhazi, Akos Ancsin, Otto Varga (students / video artists at the University of Debrecen, Hungary and highschool students – refer to themselves as the Lightscribblers) Soundtrack: Akos Szilagyi (student and composer from the University of Szeged, Hungary, who goes under the name of Rise of Day) Summary: This video was made as part of the outreach needs for the European Commission Marie Curie Actions of which CERN is a major beneficiary. The emphasis is on reaching out to communicate science and advertise the projects to the general public. The specific aim of this video is to inspire university graduates to start a research career in science and technology. As CERN is such a major beneficiary of funding for the Marie Curie well as to reach the general public who are not aware of CERN and Marie Curie opportunities. Recent experience has shown that there is a lack of good candidates and this video is intended to create an awareness among graduates that CERN and the Marie Cur...

  8. New Roles of Syntaxin-1A in Insulin Granule Exocytosis and Replenishment.

    Science.gov (United States)

    Liang, Tao; Qin, Tairan; Xie, Li; Dolai, Subhankar; Zhu, Dan; Prentice, Kacey J; Wheeler, Michael; Kang, Youhou; Osborne, Lucy; Gaisano, Herbert Y

    2017-02-10

    In type-2 diabetes (T2D), severely reduced islet syntaxin-1A (Syn-1A) levels contribute to insulin secretory deficiency. We generated β-cell-specific Syn-1A-KO (Syn-1A-βKO) mice to mimic β-cell Syn-1A deficiency in T2D. Glucose tolerance tests showed that Syn-1A-βKO mice exhibited blood glucose elevation corresponding to reduced blood insulin levels. Perifusion of Syn-1A-βKO islets showed impaired first- and second-phase glucose-stimulated insulin secretion (GSIS) resulting from reduction in readily releasable pool and granule pool refilling. To unequivocally determine the β-cell exocytotic defects caused by Syn-1A deletion, EM and total internal reflection fluorescence microscopy showed that Syn-1A-KO β-cells had a severe reduction in the number of secretory granules (SGs) docked onto the plasma membrane (PM) at rest and reduced SG recruitment to the PM after glucose stimulation, the latter indicating defects in replenishment of releasable pools required to sustain second-phase GSIS. Whereas reduced predocked SG fusion accounted for reduced first-phase GSIS, selective reduction of exocytosis of short-dock (but not no-dock) newcomer SGs accounted for the reduced second-phase GSIS. These Syn-1A actions on newcomer SGs were partly mediated by Syn-1A interactions with newcomer SG VAMP8.

  9. Luteibacter rhizovicinus gen. nov., sp nov., a yellow-pigmented gammaproteobacterium isolated from the rhizosphere of barley (Hordeum vulgare L.)

    DEFF Research Database (Denmark)

    Johansen, Jens E.; Binnerup, Svend J.; Kroer, Niels

    2005-01-01

    at 4-30 degrees C, pH 6-9 and 0-3% (w/v) NaCl. The strains had identical 16S rRNA gene sequences and ERIC (enterobacterial repetitive intergenic consensus sequence) fingerprint profiles, but could be differentiated by their RAPID (random amplified polymorphic DNA) fingerprint patterns. Strain U96(T...... % to Frateuria aurantia DSM 6220(T) and 96 % to Fulvimonas soli LMG 19981(T). Using LJ96(T) DNA as probe, DNA-DNA hybridizations documented the relationship of the three strains to a single species (87.4-98.7% relatedness) and showed less than 30% relatedness to Frateuria aurantia DSM 62207 and Fulvimonas soli...... DSM 14263(T). Rhodanobacter lindaniclasticus LMG 183857 is not extant and the strain not available from any public strain collections, thus DNA-DNA hybridization could not include this strain. On the basis of genotypic and phenotypic characteristics, the three yellow-pigmented strains could also...

  10. Comparison of next-generation sequencing systems.

    Science.gov (United States)

    Liu, Lin; Li, Yinhu; Li, Siliang; Hu, Ni; He, Yimin; Pong, Ray; Lin, Danni; Lu, Lihua; Law, Maggie

    2012-01-01

    With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world's biggest sequencing capacity, has multiple NGS systems including 137 HiSeq 2000, 27 SOLiD, one Ion Torrent PGM, one MiSeq, and one 454 sequencer. We have accumulated extensive experience in sample handling, sequencing, and bioinformatics analysis. In this paper, technologies of these systems are reviewed, and first-hand data from extensive experience is summarized and analyzed to discuss the advantages and specifics associated with each sequencing system. At last, applications of NGS are summarized.

  11. Aplicação de cinzas residuais e de fibra de sisal na produção de argamassas e concretos: Revisão

    OpenAIRE

    Indara Soto Izquierdo; Marcio Antonio Ramalho

    2014-01-01

    The industrial development linked to the growing addiction to con - sumption, obsession with fossil fuels, and general desire for short term profit, cause a certain insensitivity to more sustainable alternatives. The construction industry is a sector in which the incorporation of various types of waste presents as favorable. Three types of waste with different characteristics and origins have proved attractive and advantageous for many developing countries. They are incinerated municipal soli...

  12. Archeological Data Recovery at Site 16SJB29, Near Willow Bend, St. John The Baptist Parish, Louisiana with a Discussion of Regional Archeological Expectations and Priorities Along the Mississippi River

    Science.gov (United States)

    1989-04-01

    pattern. By the turn of the century, Karlstein was known as Bonnet Carre Point. The town of St. John was renamed " Edgar ,’ after postmaster Edgar ...Parret in 1850. The Ŕ" was added because another Mississippi River post office was called Edgar (St. John the Baptist Parish Developmental Board 1959...later Mendez who purchased the Solis plantation, grew the cane and produced the tafia to distill rum. It was Mendez’s sugar maker, chemist Antoine Morin

  13. Reform of Budgeting for Acquisition: Lessons from Private Sector Capital Budgeting for the Department of Defense

    Science.gov (United States)

    2006-09-30

    Planning Execution Budgeting Programming Planning Execution Budgeting Programming Planning After Solis 2004, 28 However, the story that these diagrams...budget be separated from the overall defense budget. This separation: would help prevent the kind of financial whiplash that causes cost overruns...notes that after nearly twenty years of reform since the Packard Commission Report and Goldwater-Nichols, “major weapons systems still cost too much

  14. Characterization of Uterine Derived Growth Inhibitor (UDGI): A Novel Growth Inhibitor of Estrogen Receptor Negative Breast Cancer Cells

    Science.gov (United States)

    2000-06-01

    pellucida domain in the carboxyl terminal region. This domain appears to be involved in sperm -binding function and sperm -egg recognition (53). In the...expression in the granulosa cells surrounding the egg may serve as an adhesion molecule for cell-cell interaction and as a target for egg- sperm recognition as...Solis, D., Sanz, L., Diaz-Maurino, T., Schafer, W., Mann, K., and Topfer- Petersen, E. Characterization of two glycosylated boar spermadhesins

  15. Interazioni piante-insetti mediate da semiochimici

    OpenAIRE

    Fabbri, Elisa

    2008-01-01

    Numerosi parassitoidi localizzano i propri ospiti sfruttando le miscele di composti volatili rilasciate dalle piante infestate, che sono segnali più facilmente reperibili nell’ambiente rispetto agli odori emessi dai soli fitofagi. Anche Diglyphus isaea (Walker), un ectoparassitoide paleartico impiegato nella lotta biologica contro vari fillominatori, localizza l’ospite Liriomyza trifolii (Burgess) (Diptera Agromyzidae) sfruttando gli odori emessi dal complesso pianta (Phaseolus vulgaris L.) –...

  16. Solid state proton conductors properties and applications in fuel cells

    CERN Document Server

    Knauth, Philippe

    2012-01-01

    Proton conduction can be found in many different solid materials, from organic polymers at room temperature to inorganic oxides at high temperature. Solid state proton conductors are of central interest for many technological innovations, including hydrogen and humidity sensors, membranes for water electrolyzers and, most importantly, for high-efficiency electrochemical energy conversion in fuel cells. Focusing on fundamentals and physico-chemical properties of solid state proton conductors, topics covered include: Morphology and Structure of Solid Acids Diffusion in Soli

  17. Drug: D03940 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D03940 Drug Eculizumab (genetical recombination) (JAN); Eculizumab (USAN/INN); Soli...gic organisms and parasites 63 Biological preparations 639 Miscellaneous 6399 Others D03940 Eculizumab (genetica...IMMUNOSUPPRESSANTS L04AA Selective immunosuppressants L04AA25 Eculizumab D03940 Eculizumab (genetical recomb...08310] Others Complement system C5 [HSA:727] [KO:K03994] Eculizumab [ATC:L04AA25] D03940 Eculizumab (genetica

  18. Milk feeding, solid feeding, and obesity risk:a review of the relationships between early life feeding practices and later adiposity

    OpenAIRE

    Farrow, Claire; Haycraft, Emma; Mitchell, Gemma L.

    2013-01-01

    Childhood obesity is a major health issue with associated ill-health consequences during childhood and into later adolescence and adulthood. Given that eating behaviors are formed during early childhood, it is important to evaluate the relationships between early life feeding practices and later child adiposity. This review describes and evaluates recent literature exploring associations between child weight and the mode of milk feeding, the age of introducing solid foods and caregivers’ soli...

  19. Improving Deployment-Related Primary Care Provider Assessments of PTSD and Mental Health Conditions

    Science.gov (United States)

    2011-10-01

    A power primer. Psychological Bulletin, 112(1), 155-159. Fallowfield, L., Jenkins, V., Farewell, V., & Solis-Trapala, I. Enduring impact of...design for multisite randomized trials. Psychological Methods, 5(2), 199-213. Roter, D. L., Larson, S., Shinitzky, H., Chernoff, R., Serwint, J. R...don’t want to miss any work. You want to find a yoga class or something you can do to help relax. Coping strategies Prayer. Prays to God for

  20. Ca3Co4O9+δ, a growing potential SOFC cathode material: impact of the layer composition and thickness on the electrochemical properties

    NARCIS (Netherlands)

    Rolle, A.; Abbas, H.A.A.; Huo, D.; Capoen, E.; Mentré, O.; Vannier, R.N.; Daviero-Minaud, S.; Boukamp, B.A.

    2016-01-01

    The thermoelectric material Ca3Co4O9 + δ (CCO), with an electronic conductivity of σe = 240 S·cm− 1 at 650 °C and a good chemical and mechanical compatibility with the standard Ce0.9Gd0.1O1.95 electrolyte (CGO, TEC: 9–10 · 10− 6 K− 1), was recently identified as a potential cathode material for soli

  1. Maternal Intravenous Treatment with either Azithromycin or Solithromycin Clears Ureaplasma parvum from the Amniotic Fluid in an Ovine Model of Intrauterine Infection

    OpenAIRE

    2014-01-01

    Intrauterine infection with Ureaplasma spp. is strongly associated with preterm birth and adverse neonatal outcomes. We assessed whether combined intraamniotic (IA) and maternal intravenous (IV) treatment with one of two candidate antibiotics, azithromycin (AZ) or solithromycin (SOLI), would eradicate intrauterine Ureaplasma parvum infection in a sheep model of pregnancy. Sheep with singleton pregnancies received an IA injection of U. parvum serovar 3 at 85 days of gestational age (GA). At 12...

  2. Citizen or Subordinate: Permutations of Belonging in the United States and the Dominican Republic

    Directory of Open Access Journals (Sweden)

    Shaina Aber

    2013-09-01

    Full Text Available The Dominican Republic and the United States have both experienced tensions arising from migratory flows from poorer, less stable neighbors. Until recently, both countries had constitutions which conferred citizenship by birth with very limited exceptions. Despite these similarities, their respective discourses around jus soli citizenship, particularly for the children of unauthorized migrants from the poorer neighboring countries, have manifested in different ways. The identity of the United States as a nation of immigrants has limited the success of campaigns to revoke jus soli citizenship for the children of unauthorized immigrants, but the persistent articulation of this idea as a response to illegal migration has shifted the parameters of the immigration debate. In the Dominican Republic, the historical construction of national identity and anti-Haitian discourse has led to an evolution in Dominican law which codifies already established practices that deny citizenship to children of Haitian migrants. In both cases, movements that support more inclusive understandings of societal belonging, like the DREAMers in the United States and youth movements in the Dominican Republic, may offer the most effective way of protecting universal jus soli citizenship regimes. 

  3. Comparison of sequencing platforms for single nucleotide variant calls in a human sample.

    Science.gov (United States)

    Ratan, Aakrosh; Miller, Webb; Guillory, Joseph; Stinson, Jeremy; Seshagiri, Somasekar; Schuster, Stephan C

    2013-01-01

    Next-generation sequencings platforms coupled with advanced bioinformatic tools enable re-sequencing of the human genome at high-speed and large cost savings. We compare sequencing platforms from Roche/454(GS FLX), Illumina/HiSeq (HiSeq 2000), and Life Technologies/SOLiD (SOLiD 3 ECC) for their ability to identify single nucleotide substitutions in whole genome sequences from the same human sample. We report on significant GC-related bias observed in the data sequenced on Illumina and SOLiD platforms. The differences in the variant calls were investigated with regards to coverage, and sequencing error. Some of the variants called by only one or two of the platforms were experimentally tested using mass spectrometry; a method that is independent of DNA sequencing. We establish several causes why variants remained unreported, specific to each platform. We report the indel called using the three sequencing technologies and from the obtained results we conclude that sequencing human genomes with more than a single platform and multiple libraries is beneficial when high level of accuracy is required.

  4. Hlf is a genetic modifier of epilepsy caused by voltage-gated sodium channel mutations.

    Science.gov (United States)

    Hawkins, Nicole A; Kearney, Jennifer A

    2016-01-01

    Mutations in voltage-gated sodium channel genes cause several types of human epilepsies. Often, individuals with the same sodium channel mutation exhibit diverse phenotypes. This suggests that factors beyond the primary mutation influence disease severity, including genetic modifiers. Mouse epilepsy models with voltage-gated sodium channel mutations exhibit strain-dependent phenotype variability, supporting a contribution of genetic modifiers in epilepsy. The Scn2a(Q54) (Q54) mouse model has a strain-dependent epilepsy phenotype. Q54 mice on the C57BL/6J (B6) strain exhibit delayed seizure onset and improved survival compared to [B6xSJL/J]F1.Q54 mice. We previously mapped two dominant modifier loci that influence Q54 seizure susceptibility and identified Hlf (hepatic leukemia factor) as a candidate modifier gene at one locus. Hlf and other PAR bZIP transcription factors had previously been associated with spontaneous seizures in mice thought to be caused by down-regulation of the pyridoxine pathway. An Hlf targeted knockout mouse model was used to evaluate the effect of Hlf deletion on Q54 phenotype severity. Hlf(KO/KO);Q54 double mutant mice exhibited elevated frequency and reduced survival compared to Q54 controls. To determine if direct modulation of the pyridoxine pathway could alter the Q54 phenotype, mice were maintained on a pyridoxine-deficient diet for 6 weeks. Dietary pyridoxine deficiency resulted in elevated seizure frequency and decreased survival in Q54 mice compared to control diet. To determine if Hlf could modify other epilepsies, Hlf(KO/+) mice were crossed with the Scn1a(KO/+) Dravet syndrome mouse model to examine the effect on premature lethality. Hlf(KO/+);Scn1a(KO/+) offspring exhibited decreased survival compared to Scn1a(KO/+) controls. Together these results demonstrate that Hlf is a genetic modifier of epilepsy caused by voltage-gated sodium channel mutations and that modulation of the pyridoxine pathway can also influence phenotype

  5. Polyphenolic compounds and essential oil analysis of selected species of the genus Thymus / Analýza fenolových zložiek a analýza silice vybraných druhov rodu Thymus

    Directory of Open Access Journals (Sweden)

    Kameníková M.

    2015-06-01

    Full Text Available Predložena praca bola zamerana na stanovenie obsahu vybranych fenolovych zlučenin (celkove fenoly, triesloviny, flavonoidy a antokyany troch roznych druhov rodu Thymus (T. pulegioides, T. pannonicus, T. praecox s rozdielnym povodom (Českomoravska vrchovina, Křivoklat a Považsky Inovec. Na stanovenie tychto zložiek boli využite spektrofotometricke metody Europskeho liekopisu, 8. vydanie. Zaroveň bol stanoveny aj obsah silice a bola vykonana analyza jej zložiek. Obsah celkovych polyfenolov bol stanoveny spektrofotometrickou metodou s využitim Folin-Ciocalteovho činidla. Obsah kolisal od 3.87 % do 8,86 %. Obsah trieslovin bol realizovany na zaklade predošleho stanovenia obsahu celkovych fenolov, a to adsorpciou trieslovin na kožny prašok. Ich obsah sa pohyboval v rozmedzi 1,96 % do 5,65 %. Na stanovenie obsahu flavonoidov bola využita spektrofotometricka metoda s chloridom hlinitym. Obsah flavonoidov vyjadrenych ako luteolin-7-O-glukozid (λ = 392 nm kolisal od 0,59 % do 1,52 % a obsah flavonoidov vyjadrenych ako rutin (λ = 420 nm sa pohyboval v rozmedzi od 0,41 % do 1,12 %. Antokyany zaberaju v ramci fenolovych zložiek rodu Thymus len minimalny podiel. V našich vzorkach bol obsah antokyanov vyjadreny ako cyanidin-3- O-glukozid a kolisal od 0,02 % do 0,1 %. Na stanovenie obsahu silice bola využita metoda Europskeho liekopisu, 8.vydanie. Obsah silice sa pohyboval v rozmedzi od 0,2 % do 0,75 %. Na analyzu silice bola nasledne využita metoda plynovej chromatografie s hmotnostnou spektrometriou. Na zaklade tejto analyzy bola zistena pritomnosť troch chemotypov silice, a to tymoloveho, karvakroloveho a linalooloveho chemotypu. Vďaka variaciami chemickeho zloženia silice, bolo možne pozorovať vzťah medzi pritomnosťou určiteho chemotypu a povodom vzorky. Rozdiely v stanoveni obsahu flavonoidov nepreukazali žiadny vyrazny vzťah k lokalite povodu.

  6. Dijalektika samoće

    OpenAIRE

    Paz, Octavio

    1991-01-01

    Samoća - osjećaj i spoznaja da si sam, otuđen od svijeta i od sebe sama - nije isključivo meksička osobina. Svi se ljudi, u nekom trenutku života, osjećaju usamljenima. A oni to i jesu, živjeti znači biti odijeljen od onoga što smo bili da bismo se približili onome što ćemo biti u tajnovitoj budućnosti. Samoća je najdublja činjenica ljudskog postojanja. Čovjek je jedno biće koje zna da je samo i jedino koje traži drugo. Njegova priroda - ako se ta riječ može upotrijebiti u odnosu na čovjeka, ...

  7. Využití návrhového vzoru Model-View-Controller ve webových aplikacích

    OpenAIRE

    Margai, Ladislav

    2013-01-01

    Obsah práce je zameraný na využitie návrhového vzoru Model-View-Controller vo webových aplikáciach. Práca je rozdelená do niekoľkých kapitol. V prvej kapitole je vysvetlený model MVC ako taký a nutné úpravy interpretácie pre použitie vo webových aplikáciach. V druhej kapitole je popísaný vývoj od konceptu webových formulárov až po MVC a porovnanie týchto dvoch technológií. Tretia kapitola sa zameriava na prácu s Razor engine. Posledné tri kapitoly popisujú samostatný vývoj aplikácie. Conte...

  8. Rodinný dům

    OpenAIRE

    Uhrinec, Marek

    2014-01-01

    Bakalárska práca je projekt rodinného domu v meste Louny. Rodinný dom je projektovaný ako dvojpodlažný dom s jedným podzemným podlažím. Budova je navrhnutá pre štyri osoby. Dom má plochú strechu. Nadzemné murivo je tvorené systémom POROTHERM. Nosné múry v prvom podzemnom poschodí sú tvorené systémom Prefa BTB. Priečky sú taktiež tvorené systémom POROTHERM. Hlavný vstup do budovy je situovaný na severovýchod. V suteréne sa nachádza technická miestnosť, sušiareň, sklad zeleniny, sklad, chodba a...

  9. Návrh internetových stránek

    OpenAIRE

    Chomo, Martin

    2009-01-01

    Táto bakalárska práca sa zaoberá návrhom internetových stránok pre novovznikajúcu firmu za účelom jej propagácie a prieniku na inak ťažko dostupné trhy. Obsah práce popisuje postup pri vytváraní internetovej prezentácie firmy, grafický design, samotné programovanie stránok ako aj umiestnenie stránok na internet a návrh ich zviditežnenia prostredníctvom i-marketingu. Nakoniec sa táto bakalárska práca zaoberá finančným zhodnotením navrhovaného projektu- nákladov a predpokladaných prínosov. T...

  10. Minimally invasive veneers: current state of the art

    Directory of Open Access Journals (Sweden)

    Vanlıoğlu BA

    2014-11-01

    Full Text Available Burçin Akoğlu Vanlioğlu, Yasemin Kulak-Özkan University of Marmara, Department of Prosthetic Dentistry, Istanbul, TurkeyAbstract: Ceramic veneers are considered a conservative solution for patients requiring improvement of the shape, color, or position of their anterior teeth. Ceramic veneers have been extensively and successfully used to mask intrinsic staining, to give the appearance of straightening, and to correct minor malformations of anterior teeth without the removal of substantial amounts of sound tooth substance. The current literature was reviewed to search for the most important parameters determining the long-term success and correct application of ceramic veneers. Keywords: ceramic veneers, esthetic treatment

  11. Restauracije mliječnih zuba cirkonijskim krunicama kod djece s ranim karijesom

    OpenAIRE

    Karaca, Serhat; Ozbay, Gizem; Kargul, Betul

    2013-01-01

    Rani dječji karijes (ECC) razarajući je oblik truljenja zuba koji pogađa predškolsku djecu. Obično se pojavljuje na mliječnim maksilarnim sjekutićima dojenčadi i vrlo male djece. Ti su zubi vrlo važni za fizički izgled, pa ako nestane njihova struktura to ne utječe samo na estetiku nego kompromitira i žvakanje, a uzrokuje i teškoće u socijalnoj i psihološkoj prilagodbi djeteta. Estetska restauracija mliječnih sjekutića može se obaviti različitim materijalima, primjerice, gotovim cirkonij...

  12. KRIŽOBOLJA I OZLJEDE MALIH ZGLOBOVA TE LIGAMENTARNO-MIŠIĆNOG APARATA LUMBALNE KRALJEŽNICE U SPORTAŠA

    OpenAIRE

    2007-01-01

    SAŽETAK Bavljenje sportom izlaže kralježnicu, posebno njezin lumbalni dio, povećanom opterećenju, zbog čega su u sportaša česte ozljede ligamentarno-mišićnog aparata i zglobova kralježnice. Do njihova oštećenja najčešće dolazi zbog naglih i snažnih kretnji – istezanja i rotacije, kojima su izloženi u sportu. Glavni je simptom križobolja praćena spazmom paravertebralne muskulature. Liječenje je konzervativno te ako se pravodobno ne započne s odgovarajućom terapijom m...

  13. Vision system for dial gage torque wrench calibration

    Science.gov (United States)

    Aggarwal, Neelam; Doiron, Theodore D.; Sanghera, Paramjeet S.

    1993-11-01

    In this paper, we present the development of a fast and robust vision system which, in conjunction with the Dial Gage Calibration system developed by AKO Inc., will be used by the U.S. Army in calibrating dial gage torque wrenches. The vision system detects the change in the angular position of the dial pointer in a dial gage. The angular change is proportional to the applied torque. The input to the system is a sequence of images of the torque wrench dial gage taken at different dial pointer positions. The system then reports the angular difference between the different positions. The primary components of this vision system include modules for image acquisition, linear feature extraction and angle measurements. For each of these modules, several techniques were evaluated and the most applicable one was selected. This system has numerous other applications like vision systems to read and calibrate analog instruments.

  14. Optimalizácia monitorovania sieťovej prevádzky

    Directory of Open Access Journals (Sweden)

    František Jakab

    2013-06-01

    Full Text Available Tento príspevok sa zaoberá otvorenými problémami vyskytujúcimi sa pri pasívnom prístupe merania sieťových charakteristík. Opisuje najpoužívanejšie prístupy merania sieťových parametrov ako aj charakteristiky, ktoré sa pri monitorovaní najčastejšie sledujú. Hlavným cieľom tohto príspevku je predstavenie konceptuálneho návrhu riešenia opísaných problémov, ktorý by sa mal docieliť automatizovaným prispôsobením exportu záznamov o tokoch prevádzky k aktuálnemu stavu siete.

  15. Snaha o udržateľný spôsob života sa začína na tanieri každého Slováka

    Directory of Open Access Journals (Sweden)

    Diana Rodáková

    2011-12-01

    Full Text Available „V dňoch ako je tento si znovu uvedomujem, čo som vám tvrdil už stokrát: so svetom nie je nič v neporiadku. V neporiadku je náš pohľad na svet.“ Henry Miller Aj toto je jeden z citátov, ktoré na mňa ako na mladého a stále tvárneho človeka zapôsobili. Neraz sa práve moja generácia nazýva apatickou, bez snahy niečo zmeniť, vyrastajúcou v obkľúčení kníh, tabuliek, vzorcov a iných bariér, chrániacich ju pred skutočným svetom a uvedomením si priorít. Téma smerovania Slovenska v uplynulom desaťročí je pre mňa aktuálnou hlavne z toho dôvodu, že vzhľadom na môj vek práve toto obdobie intenzívnejšie vnímam a nie som zdeformovaná staršími názormi na ekológiu a trvalú udržateľnosť, od ktorých sa staršia generácia často nedokáže odosobniť.

  16. Estimation of Avalanche Hazard in the Settlement of Magurka using Elba+ Model / Posúdenie Ohrozenosti Osady Magurky Lavínami S Použitím Modelu Elba+

    Directory of Open Access Journals (Sweden)

    Bartík Martin

    2013-06-01

    Full Text Available V našej študií sme sa zamerali na moderné softvérové aplikácie umožňujúce simuláciu lavíny. Použili sme model ELBA+, pomocou ktorého sme snažili zhodnotiť ohrozenosť horského prostredia v okolí starej banskej osady Magurka (1 036 m n. m., ktorá leží v závere Ľupčianskej doline pod hlavným hrebeňom Nízkych Tatier. Do histórie sa zapísala hlavne lavína v doline Ďurková zo 14. marca 1970, ktorá dodnes patrí medzi najväčšie lavíny zaznamenané na Slovensku. S použitím archívnych údajov Strediska lavínovej prevencie v Jasnej sme sa pokúsili o jej čo najvernejšiu rekonštrukciu. Následne sme sa pokúsili o simuláciu lavíny v doline Viedenka pri použití rovnakej výšky snehu v odtrhovom pásme ako sa predpokladá pri lavíne v roku 1970. Keďže časť osady Magurka je situovaných pri ústí tejto doliny, ktorá má priamejší aj kratší priebeh ako dolina Ďurková, skúmali sme možnosť jej zásahu lavínou.

  17. Posmrtna oprema

    Directory of Open Access Journals (Sweden)

    Fernando Iwasaki

    2015-06-01

    Full Text Available Časna majka podigne pogled prema nebu kao da na njemu traži Božji znak dok joj je u očima oteklim od cjelonoćne molitve zablistala suza.– Kažete, sestro, da stari profesor odbija ići na misu?– Tako je, časna majko. Osim toga proklinje i vrijeđa presvetu Djevicu Mariju.– Nije važno, sestro. Odvedite ga u šetnju vrtom.– Da, časna majko.– Sestro…– Molim, časna majko?– Neka izgleda kao nesreća. Jedne noći nisam mogao zaspati, pa mi je mama stavila pod jastuk knjigu Put u središte Zemlje, rekla je da ću, ako brzo zaspim, sanjati baš te pustolovine. I kako sam te noći sanjao da sam se spustio do samog središta Zemlje, otada svake noći pod jastuk stavljam knjige, stripove i časopise koje želim sanjati. Kada sam se upisao na fakultet, oduševljeno sam otkrio da trik funkcionira s bilješkama, videosnimkama i fotografijama mojih kolegica. Tako sam diplomirao s najboljim ocjenama, zaradio gomilu novca i ostvario što god sam si zacrtao, sve dok mi večeras supruga nije zaprijetila da će me ostaviti ako ne bacim u smeće taj prastari jastuk iz djetinjstva. Jedva sam je uspio nagovoriti da na njemu odspava do jutra i sama otkrije zbog čega mi se toliko sviđa.

  18. Importance of automatic threshold for image segmentation for accurate measurement of fine roots of woody plants / Význam automatického prahovania na obrazovú segmentáciu pre presné merania jemných koreňov drevín

    Directory of Open Access Journals (Sweden)

    Surový Peter

    2014-12-01

    Full Text Available Jemné korene sú považované za kľúčové orgány zabezpečujúce prežitie rastliny, jej rast a produkciu. Merania jemných koreňov sú ľahko a pohodlne vykonávateľné pomocou digitálneho obrazu. Popisné veličiny ako plocha koreňov, ich povrch, celková dĺžka či hrúbková štruktúra sa dajú získať z digitálneho obrazu. Analýza digitálneho obrazu pozostáva z niekoľkých krokov, z ktorých každý predstavuje potenciálny zdroj chyby merania. V tomto článku sa zameriavame na automatickú segmentáciu prahovaním a jej vplyv na veličiny získavané z digitálnych skenov jemných koreňov korkového duba (Quercus suber L.. Porovnávame 16 rôznych prahovacích metód a ich správnosť v porovnaní s binárnymi obrazmi vytvorenými ľudským hodnotiteľom. Zistili sme, že niektoré prahovacie metódy dávajú lepšie výsledky ako ostatné v odhade plochy koreňov, ale pred odhad dĺžky koreňov je poradie metód podľa ich správnosti len mierne odlišné

  19. Genome-wide SNP discovery in walnut with an AGSNP pipeline updated for SNP discovery in allogamous organisms

    Directory of Open Access Journals (Sweden)

    You Frank M

    2012-07-01

    Full Text Available Abstract Background A genome-wide set of single nucleotide polymorphisms (SNPs is a valuable resource in genetic research and breeding and is usually developed by re-sequencing a genome. If a genome sequence is not available, an alternative strategy must be used. We previously reported the development of a pipeline (AGSNP for genome-wide SNP discovery in coding sequences and other single-copy DNA without a complete genome sequence in self-pollinating (autogamous plants. Here we updated this pipeline for SNP discovery in outcrossing (allogamous species and demonstrated its efficacy in SNP discovery in walnut (Juglans regia L.. Results The first step in the original implementation of the AGSNP pipeline was the construction of a reference sequence and the identification of single-copy sequences in it. To identify single-copy sequences, multiple genome equivalents of short SOLiD reads of another individual were mapped to shallow genome coverage of long Sanger or Roche 454 reads making up the reference sequence. The relative depth of SOLiD reads was used to filter out repeated sequences from single-copy sequences in the reference sequence. The second step was a search for SNPs between SOLiD reads and the reference sequence. Polymorphism within the mapped SOLiD reads would have precluded SNP discovery; hence both individuals had to be homozygous. The AGSNP pipeline was updated here for using SOLiD or other type of short reads of a heterozygous individual for these two principal steps. A total of 32.6X walnut genome equivalents of SOLiD reads of vegetatively propagated walnut scion cultivar ‘Chandler’ were mapped to 48,661 ‘Chandler’ bacterial artificial chromosome (BAC end sequences (BESs produced by Sanger sequencing during the construction of a walnut physical map. A total of 22,799 putative SNPs were initially identified. A total of 6,000 Infinium II type SNPs evenly distributed along the walnut physical map were selected for the

  20. High-resolution analysis of the 5'-end transcriptome using a next generation DNA sequencer.

    Directory of Open Access Journals (Sweden)

    Shin-ichi Hashimoto

    Full Text Available Massively parallel, tag-based sequencing systems, such as the SOLiD system, hold the promise of revolutionizing the study of whole genome gene expression due to the number of data points that can be generated in a simple and cost-effective manner. We describe the development of a 5'-end transcriptome workflow for the SOLiD system and demonstrate the advantages in sensitivity and dynamic range offered by this tag-based application over traditional approaches for the study of whole genome gene expression. 5'-end transcriptome analysis was used to study whole genome gene expression within a colon cancer cell line, HT-29, treated with the DNA methyltransferase inhibitor, 5-aza-2'-deoxycytidine (5Aza. More than 20 million 25-base 5'-end tags were obtained from untreated and 5Aza-treated cells and matched to sequences within the human genome. Seventy three percent of the mapped unique tags were associated with RefSeq cDNA sequences, corresponding to approximately 14,000 different protein-coding genes in this single cell type. The level of expression of these genes ranged from 0.02 to 4,704 transcripts per cell. The sensitivity of a single sequence run of the SOLiD platform was 100-1,000 fold greater than that observed from 5'end SAGE data generated from the analysis of 70,000 tags obtained by Sanger sequencing. The high-resolution 5'end gene expression profiling presented in this study will not only provide novel insight into the transcriptional machinery but should also serve as a basis for a better understanding of cell biology.

  1. Comparison of sequence reads obtained from three next-generation sequencing platforms.

    Directory of Open Access Journals (Sweden)

    Shingo Suzuki

    Full Text Available Next-generation sequencing technologies enable the rapid cost-effective production of sequence data. To evaluate the performance of these sequencing technologies, investigation of the quality of sequence reads obtained from these methods is important. In this study, we analyzed the quality of sequence reads and SNP detection performance using three commercially available next-generation sequencers, i.e., Roche Genome Sequencer FLX System (FLX, Illumina Genome Analyzer (GA, and Applied Biosystems SOLiD system (SOLiD. A common genomic DNA sample obtained from Escherichia coli strain DH1 was applied to these sequencers. The obtained sequence reads were aligned to the complete genome sequence of E. coli DH1, to evaluate the accuracy and sequence bias of these sequence methods. We found that the fraction of "junk" data, which could not be aligned to the reference genome, was largest in the data set of SOLiD, in which about half of reads could not be aligned. Among data sets after alignment to the reference, sequence accuracy was poorest in GA data sets, suggesting relatively low fidelity of the elongation reaction in the GA method. Furthermore, by aligning the sequence reads to the E. coli strain W3110, we screened sequence differences between two E. coli strains using data sets of three different next-generation platforms. The results revealed that the detected sequence differences were similar among these three methods, while the sequence coverage required for the detection was significantly small in the FLX data set. These results provided valuable information on the quality of short sequence reads and the performance of SNP detection in three next-generation sequencing platforms.

  2. The 'reformation' of counselling

    Directory of Open Access Journals (Sweden)

    G.A. Lotter

    2001-08-01

    Full Text Available Although the Reformation took place some four hundred years ago, one area in which reformation is really needed today is the counselling of people. Since Wilhelm Wundt started the “study of the mind” in 1879, William James and Sigmund Freud followed and secular psychology gradually has developed to take the “front seat”; hence moving Biblical counselling, which has been practised since the times of the New Testament, to the “back burner”. This development had been going on for the greater part of the 20th century, up to the publication of Competent to Counsel by Jay E. Adams in 1970. In the model for counselling suggested by Adams, the principles of the Reformation of the sixteenth century, Soli Deo Gloria, Soli Scriptura, Soli Fidei, Sola Gratia, etc. were again implemented in assisting and counselling people with personal and interpersonal problems. The epistomological and anthropological approach of secular psychology differs radically from that of Biblical principles, thus necessitating a new “reformation” of counselling. Within this new form counselling, inter alia, implies the following: the Word of God has its rightful place, sin has to be taken seriously and the work of the Holy Spirit should be recognised. In this article it is proposed that the “reformation” of counselling was started by scholars with a Biblical Reformational approach and that this method of counselling followed the parameters of the Reformation of the sixteenth century. This “reformation” developed into a new direction in counselling and still continues today with fascinating new frontiers opening up for Biblical counselling.

  3. HMI Synoptic Maps Produced by NSO/NISP

    CERN Document Server

    Hughes, Anna L H; Marble, Andrew R; Oien, Niles A; Petrie, Gordon; Pevtsov, Alexei A

    2016-01-01

    Recently, the National Solar Observatory (NSO) Solar-atmosphere Pipeline Working Group has undertaken the production of synoptic maps from Helioseismic and Magnetic Imager (HMI) magnetograms. A set of maps has been processed spanning the data available for 2010-2015 using twice daily images (taken at UT midnight and noon) and running them through the same algorithms used to produce SOLIS/VSM 6302l mean-magnetic and spatial-variance maps. The contents of this document provide an overview of what these maps look like, and the processing steps used to generate them from the original HMI input data.

  4. Coverage bias and sensitivity of variant calling for four whole-genome sequencing technologies.

    Directory of Open Access Journals (Sweden)

    Nora Rieber

    Full Text Available The emergence of high-throughput, next-generation sequencing technologies has dramatically altered the way we assess genomes in population genetics and in cancer genomics. Currently, there are four commonly used whole-genome sequencing platforms on the market: Illumina's HiSeq2000, Life Technologies' SOLiD 4 and its completely redesigned 5500xl SOLiD, and Complete Genomics' technology. A number of earlier studies have compared a subset of those sequencing platforms or compared those platforms with Sanger sequencing, which is prohibitively expensive for whole genome studies. Here we present a detailed comparison of the performance of all currently available whole genome sequencing platforms, especially regarding their ability to call SNVs and to evenly cover the genome and specific genomic regions. Unlike earlier studies, we base our comparison on four different samples, allowing us to assess the between-sample variation of the platforms. We find a pronounced GC bias in GC-rich regions for Life Technologies' platforms, with Complete Genomics performing best here, while we see the least bias in GC-poor regions for HiSeq2000 and 5500xl. HiSeq2000 gives the most uniform coverage and displays the least sample-to-sample variation. In contrast, Complete Genomics exhibits by far the smallest fraction of bases not covered, while the SOLiD platforms reveal remarkable shortcomings, especially in covering CpG islands. When comparing the performance of the four platforms for calling SNPs, HiSeq2000 and Complete Genomics achieve the highest sensitivity, while the SOLiD platforms show the lowest false positive rate. Finally, we find that integrating sequencing data from different platforms offers the potential to combine the strengths of different technologies. In summary, our results detail the strengths and weaknesses of all four whole-genome sequencing platforms. It indicates application areas that call for a specific sequencing platform and disallow other

  5. Toksikološki aspekti anabolika u hrani životinjskog podrijetla

    OpenAIRE

    Pleadin, Jelka; Perši, Nina; Antolović, B.; Šimić, B.; Kmetić, Ivana

    2011-01-01

    Zbog ekonomske isplativosti pri proizvodnji mesa u stočarskoj industriji zlouporabljale su se različite tvari s anaboličkim učinkom, i to stilbeni, derivati stilbena, njihove soli i esteri, antitireoidne tvari, prirodni i sintetski sterodini spolni hormoni, laktoni rezorcilne kiseline i beta-agonisti. Način djelovanja tvari s anaboličkim učinkom u organizmu razlikuje se po navedenim grupama tvari, a rezultira povećanim zadržavanjem dušika, sintezom proteina i razgradnjom masti. Iako su mehani...

  6. Design and development of batch type acetifier for wine-vinegar production

    OpenAIRE

    Singh, R.; Singh, S

    2007-01-01

    A batch type acetifier based on the principal of acetic acid fermentation was designed and tested for production of wine-vinegar from the pineapple peel waste. The pineapple peels along with starter solution was fed to the inner SS perforated peel-solid separator tank 130 mm dia having perforations of 50 mm size. The concentric perforated peel-solid separator circular tank was fitted inside the collecting tank having 255 mm dia. The pineapple peels and starter solution in perforated peel-soli...

  7. Música y matemáticas

    OpenAIRE

    Blázquez Lozano, Rosa Marina

    2014-01-01

    Durante muchos siglos se ha considerado que las matemáticas y la música tienen cierta similitud y comúnmente se dice que tienen al menos cierta relación. Ambas tienen algo de mágico, comenta Tiburcio Solis (2002), son tan abstractas que parecen pertenecer a otro mundo y sin embargo tienen gran poder en este mundo, la música afecta a la escucha y las matemáticas tienen múltiples aplicaciones prácticas. Una parte de las matemáticas estudia los números, sus patrones y formas y estos elementos...

  8. The Distrbution of Various Mercury Species in Soil

    Institute of Scientific and Technical Information of China (English)

    冯新斌; 陈业材; 等

    1997-01-01

    According to the mercury species with different solubilities,the analytical procedure involving sequential chemical extraction has been applied to partitioning the mercury species in soils into seven fractions.Soil samples collected from five localities in different areas (the high-mercury ares.the man-made mercury-polluted area and the reference area)were an alyzed for the seven mercury species.It is found that high mercury contents of soils can be attributed to both man-made pollution and geological processes,but the two kinds of solis show obvious differences in the distribution of their mercury species.

  9. Installation Restoration Program. Records Search for Phelps Collins Air National Guard Base, Alpena, Michigan

    Science.gov (United States)

    1985-07-01

    16 24 Net precipitation 1 166 1 SOLi pomeabLI’It’ 3 24 24 Subsurface flows 1 8 24 Direct access to ground water 0 0 24 Subtotals 54 114 Subscore (100...rTrichloroethylene Jt-4𔃽) In 65 Trichlorofluoromethane IND ___ _rD ND -DN0. liriv l Chloride iND IND NU ND 0.2 Results in -irg~a~r e ie LEOPOLDO L...Trichiorofluoromethane .jND 0.1_____________________ ___ Vinv Chorrdee ND 0.21.1 ... I Results in Micrograms per Liter LEOPOLDO L

  10. デカルトの物心二元論再考

    OpenAIRE

    立花, 希一; TACHIBANA, Kiichi

    2008-01-01

    Descartes (lS96-16SOlis the first figure who proposed body-mind dualism based on his own rational arguments, not based on any allegorical or philosophical interpretations of the Bible, which was primarily acceptedas the absolute authority. In this paper I critically examine Descartes' arguments, and point out his wrong strategy. that is, we ought to exclude as false all these things of which we may doubt. I claim that Descartes' philosophical opinion is not the sole absolutely certain truth, ...

  11. Finite element model of the Jefferson fracture: comparison with a cadaver model

    OpenAIRE

    Bozkus, Hakan; Karakas, Askin; Hancı, Murat; Uzan, Mustafa; Bozdag, Ergun; Sarıoglu, Ali

    2001-01-01

    This study tries to explain the reason why the Jefferson fracture is a burst fracture, using two different biomechanical models: a finite element model (FEM) and a cadaver model used to determine strain distribution in C1 during axial static compressive loading. For the FEM model, a three-dimensional model of C1 was obtained from a 29-year-old healthy human, using axial CT scans with intervals of 1.0 mm. The mesh model was composed of 8200 four-noded isoparametric tetrahedrons and 37,400 soli...

  12. Semi-solid Processing of Al-7Graghite Composite

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Al-7graphite composite was processed using Al-7graphite mush y prepared by electromagnetic-mechanical stirring method, and the infl uence of solid fraction on the distributing of graphite particles in i ngot was studied. The results shows that the relationship between soli d fraction and stirring temperature of mushy is: =591.50.897 (where i s the solid fraction, is the stirring temperature). For Al-7graphite composite, with the increasing of solid fraction, the aggregation exte nt of graphite particles reduced gradually, and when solid fraction wa s larger than 30%, graphite particles could distribute evenly in ingot .

  13. Genomic research for important pathogenic bacteria in China

    Institute of Scientific and Technical Information of China (English)

    YANG RuiFu; GUO XiaoKui; YANG Jian; JIANG YongQiang; PANG Bo; CHEN Chen; YAO YuFeng; QIN JinHong; LI QingTian

    2009-01-01

    Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of point. We can thus closely look at the way in which a pathogen is evolved, and these data has been applied to molecular epidemiology and microbial forensics, and screening of novel diagnostic, vaccine and drug targets. The newly developed high-throughput low-cost sequencing technologies, such as 454, Solexa and SOLiD, will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously, such as the metagenomics of human gastric-intestinal tract, for better and comprehensive understanding of human health and disease.

  14. Electricity from the Sahara and hydropower - moving forward with renewable energies; Saharastrom und Wasserkraft - mit erneuerbaren Energien in die Zukunft

    Energy Technology Data Exchange (ETDEWEB)

    Kolle, Christian (comp.)

    2009-07-01

    On 13 July a launch meeting took place under the auspices of Muenchner Rueck to initiate the largest private green electricity project of all times. Twenty German companies are preparing themselves to participate in the construction of photovoltaic systems and solar power plants in the Sahara. Even the technical problem of transporting the electricity to Europe, hitherto an obstacle to profitability, seems to have been brought to a solution. The following information was sourced from the GESIS databases SOLIS and SOFIS and the six English-language databases of the producer Cambridge Scientific Abstracts (CSA) which are integrated in www.sowiport.de via the national licences. The SOLIS database mostly covers German-language publications, i.e. journal contributions, monographies as well as contributions to compilations in the major social sciences. SOFIS largely procures its information through surveys among institutions in German speaking countries where social science research is conducted. The CSA databases offer references to research results centering on sociology and political science as well as neighbouring disciplines in the social and economic sciences. They largely cover international journals, but also books, book chapters and in some cases publication reviews.

  15. Orbital monitoring of martian surface changes

    Science.gov (United States)

    Geissler, Paul E.; Fenton, Lori K.; Enga, Marie-therese; Mukherjee, Priyanjoli

    2016-11-01

    A history of martian surface changes is documented by a sequence of global mosaics made up of Mars Global Surveyor Mars Orbiter Camera daily color images from 1999 to 2006, together with a single mosaic from the Mars Reconnaissance Orbiter Mars Color Imager in 2009. These observations show that changes in the global albedo patterns of Mars take place by a combination of dust storms and strong winds. Many of the observed surface changes took place along the tracks of seasonally repeating winter dust storms cataloged by Wang and Richardson (2015). These storms tend to sweep dust towards the equator, progressively shifting albedo boundaries and continuing surface changes that began before the arrival of MGS. The largest and most conspicuous changes took place during the global dust storm of 2001 (MY 25), which blanketed Syrtis Major, stripped dust from the Tharsis region, and injected dust into Solis Planum. High wind speeds but low wind stresses are predicted in Syrtis, Tharsis and Solis by the NASA Ames GCM. Frequent changes in these regions show that dust accumulations are quickly removed by stronger winds that are not predicted by the GCM, but may result from smaller-scale influences such as unresolved topography.

  16. FadE: whole genome methylation analysis for multiple sequencing platforms.

    Science.gov (United States)

    Souaiaia, Tade; Zhang, Zheng; Chen, Ting

    2013-01-01

    DNA methylation plays a central role in genomic regulation and disease. Sodium bisulfite treatment (SBT) causes unmethylated cytosines to be sequenced as thymine, which allows methylation levels to reflected in the number of 'C'-'C' alignments covering reference cytosines. Di-base color reads produced by lifetech's SOLiD sequencer provide unreliable results when translated to bases because single sequencing errors effect the downstream sequence. We describe FadE, an algorithm to accurately determine genome-wide methylation rates directly in color or nucleotide space. FadE uses SBT unmethylated and untreated data to determine background error rates and incorporate them into a model which uses Newton-Raphson optimization to estimate the methylation rate and provide a credible interval describing its distribution at every reference cytosine. We sequenced two slides of human fibroblast cell-line bisulfite-converted fragment library with the SOLiD sequencer to investigate genome-wide methylation levels. FadE reported widespread differences in methylation levels across CpG islands and a large number of differentially methylated regions adjacent to genes which compares favorably to the results of an investigation on the same cell-line using nucleotide-space reads at higher coverage levels, suggesting that FadE is an accurate method to estimate genome-wide methylation with color or nucleotide reads. http://code.google.com/p/fade/.

  17. Cyber threat impact assessment and analysis for space vehicle architectures

    Science.gov (United States)

    McGraw, Robert M.; Fowler, Mark J.; Umphress, David; MacDonald, Richard A.

    2014-06-01

    This paper covers research into an assessment of potential impacts and techniques to detect and mitigate cyber attacks that affect the networks and control systems of space vehicles. Such systems, if subverted by malicious insiders, external hackers and/or supply chain threats, can be controlled in a manner to cause physical damage to the space platforms. Similar attacks on Earth-borne cyber physical systems include the Shamoon, Duqu, Flame and Stuxnet exploits. These have been used to bring down foreign power generation and refining systems. This paper discusses the potential impacts of similar cyber attacks on space-based platforms through the use of simulation models, including custom models developed in Python using SimPy and commercial SATCOM analysis tools, as an example STK/SOLIS. The paper discusses the architecture and fidelity of the simulation model that has been developed for performing the impact assessment. The paper walks through the application of an attack vector at the subsystem level and how it affects the control and orientation of the space vehicle. SimPy is used to model and extract raw impact data at the bus level, while STK/SOLIS is used to extract raw impact data at the subsystem level and to visually display the effect on the physical plant of the space vehicle.

  18. Effector identification in the lettuce downy mildew Bremia lactucae by massively parallel transcriptome sequencing.

    Science.gov (United States)

    Stassen, Joost H M; Seidl, Michael F; Vergeer, Pim W J; Nijman, Isaäc J; Snel, Berend; Cuppen, Edwin; Van den Ackerveken, Guido

    2012-09-01

    Lettuce downy mildew (Bremia lactucae) is a rapidly adapting oomycete pathogen affecting commercial lettuce cultivation. Oomycetes are known to use a diverse arsenal of secreted proteins (effectors) to manipulate their hosts. Two classes of effector are known to be translocated by the host: the RXLRs and Crinklers. To gain insight into the repertoire of effectors used by B. lactucae to manipulate its host, we performed massively parallel sequencing of cDNA derived from B. lactucae spores and infected lettuce (Lactuca sativa) seedlings. From over 2.3 million 454 GS FLX reads, 59 618 contigs were assembled representing both plant and pathogen transcripts. Of these, 19 663 contigs were determined to be of B. lactucae origin as they matched pathogen genome sequences (SOLiD) that were obtained from >270 million reads of spore-derived genomic DNA. After correction of cDNA sequencing errors with SOLiD data, translation into protein models and filtering, 16 372 protein models remained, 1023 of which were predicted to be secreted. This secretome included elicitins, necrosis and ethylene-inducing peptide 1-like proteins, glucanase inhibitors and lectins, and was enriched in cysteine-rich proteins. Candidate host-translocated effectors included 78 protein models with RXLR effector features. In addition, we found indications for an unknown number of Crinkler-like sequences. Similarity clustering of secreted proteins revealed additional effector candidates. We provide a first look at the transcriptome of B. lactucae and its encoded effector arsenal.

  19. Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium.

    Science.gov (United States)

    Simon, Anaele; Bindschedler, Saskia; Job, Daniel; Wick, Lukas Y; Filippidou, Sevasti; Kooli, Wafa M; Verrecchia, Eric P; Junier, Pilar

    2015-11-01

    Fungi and bacteria form various associations that are central to numerous environmental processes. In the so-called fungal highway, bacteria disperse along fungal mycelium. We developed a novel tool for the in situ isolation of bacteria moving along fungal hyphae as well as for the recovery of fungi potentially involved in dispersal, both of which are attracted towards a target culture medium. We present the validation and the results of the first in situ test. Couples of fungi and bacteria were isolated from soil. Amongst the enriched organisms, we identified several species of fast-growing fungi (Fusarium sp. and Chaetomium sp.), as well as various potentially associated bacterial groups, including Variovorax soli, Olivibacter soli, Acinetobacter calcoaceticus, and several species of the genera Stenotrophomonas, Achromobacter and Ochrobactrum. Migration of bacteria along fungal hyphae across a discontinuous medium was confirmed in most of the cases. Although the majority of the bacteria for which migration was confirmed were also positive for flagellar motility, not all motile bacteria dispersed using their potential fungal partner. In addition, the importance of hydrophobicity of the fungal mycelial surface was confirmed. Future applications of the columns include targeting different types of microorganisms and their interactions, either by enrichment or by state of the art molecular biological methods.

  20. MALINA: a web service for visual analytics of human gut microbiota whole-genome metagenomic reads.

    Science.gov (United States)

    Tyakht, Alexander V; Popenko, Anna S; Belenikin, Maxim S; Altukhov, Ilya A; Pavlenko, Alexander V; Kostryukova, Elena S; Selezneva, Oksana V; Larin, Andrei K; Karpova, Irina Y; Alexeev, Dmitry G

    2012-12-07

    MALINA is a web service for bioinformatic analysis of whole-genome metagenomic data obtained from human gut microbiota sequencing. As input data, it accepts metagenomic reads of various sequencing technologies, including long reads (such as Sanger and 454 sequencing) and next-generation (including SOLiD and Illumina). It is the first metagenomic web service that is capable of processing SOLiD color-space reads, to authors' knowledge. The web service allows phylogenetic and functional profiling of metagenomic samples using coverage depth resulting from the alignment of the reads to the catalogue of reference sequences which are built into the pipeline and contain prevalent microbial genomes and genes of human gut microbiota. The obtained metagenomic composition vectors are processed by the statistical analysis and visualization module containing methods for clustering, dimension reduction and group comparison. Additionally, the MALINA database includes vectors of bacterial and functional composition for human gut microbiota samples from a large number of existing studies allowing their comparative analysis together with user samples, namely datasets from Russian Metagenome project, MetaHIT and Human Microbiome Project (downloaded from http://hmpdacc.org). MALINA is made freely available on the web at http://malina.metagenome.ru. The website is implemented in JavaScript (using Ext JS), Microsoft .NET Framework, MS SQL, Python, with all major browsers supported.

  1. Full-Disk Chromospheric Vector Magnetograms with Ca II 854.2 nm line: Some Promising Applications

    Science.gov (United States)

    Gosain, Sanjay; Harvey, J. W.; Harker, Brian; Pillet, V. M.; Pevtsov, Alexei A.; Marble, Andrew R.; Bertello, Luca; + SOLIS-Team

    2016-05-01

    Over the last decade, the focus of solar magnetometry has shifted outward from the photosphere to the chromospheric layers. The reasons for this are many. With regards to instrumentation faster detectors with more sensitivity have become available, as have fast electro-optic modulators. Also, there are several potential benefits of observing vector fields in the chromospheric layer as the magnetic field is more force-free in this layer as compared to the photosphere. Coronal force-free field extrapolations are more reliable using chromospheric fields as the lower boundary condition and free magnetic energy is readily computed using the magnetic virial theorem. Recently, a full Stokes polarimeter for the chromospheric Ca II 854.2 nm spectral line was developed and installed in the Vector Spectromagnetograph (VSM) instrument on the Synoptic Optical Long-term Investigations of the Sun (SOLIS) telescope. We present details of this new polarimeter, full disk spectropolarimetric observations and vector magnetograms of the chromosphere, and examples of some promising applications (e.g., maps of normal component of electric current density in the chromosphere, free magnetic energy estimated using virial theorem, and non-potentiality parameter magnetic shear angle).This work utilizes SOLIS data obtained by the NSO Integrated Synoptic Program (NISP), managed by the National Solar Observatory, which is operated by the Association of Universities for Research in Astronomy (AURA), Inc. under a cooperative agreement with the National Science Foundation

  2. High-Throughput Sequencing of MicroRNAs in Adenovirus Type 3 Infected Human Laryngeal Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Yuhua Qi

    2010-01-01

    Full Text Available Adenovirus infection can cause various illnesses depending on the infecting serotype, such as gastroenteritis, conjunctivitis, cystitis, and rash illness, but the infection mechanism is still unknown. MicroRNAs (miRNA have been reported to play essential roles in cell proliferation, cell differentiation, and pathogenesis of human diseases including viral infections. We analyzed the miRNA expression profiles from adenovirus type 3 (AD3 infected Human laryngeal epithelial (Hep2 cells using a SOLiD deep sequencing. 492 precursor miRNAs were identified in the AD3 infected Hep2 cells, and 540 precursor miRNAs were identified in the control. A total of 44 miRNAs demonstrated high expression and 36 miRNAs showed lower expression in the AD3 infected cells than control. The biogenesis of miRNAs has been analyzed, and some of the SOLiD results were confirmed by Quantitative PCR analysis. The present studies may provide a useful clue for the biological function research into AD3 infection.

  3. Similar foliage area but contrasting foliage biomass between young beech and spruce stands / Porovnateľná plocha avšak kontrastná biomasa asimilačných orgánov medzi mladými porastmi buka a smreka

    Directory of Open Access Journals (Sweden)

    Konôpka Bohdan

    2014-12-01

    Full Text Available Výskum sa zameral na mladé porasty buka lesného (Fagus sylvatica a smreka obyčajného (Picea abies rovnakého veku (12 rokov, veľmi podobných dimenzií stromov, rastúcich na totožnom stanovišti. Na základe odberu vzorníkov celých stromov (všetky časti okrem jemných koreňov sme skonštruovali alometrické vzťahy pre stromové komponenty. Ako nezávislá premenná sa použila hrúbka na báze kmeňa (d0. Modely vyjadrili nielen biomasu konárov, kmeňa, hrubých koreňov a asimilačných orgánov, ale aj plochu asimilačných orgánov a špecifickú listovú plochu (specific leaf area; SLA. Zistili sme, že základné morfologické vlastnosti asimilačných orgánov varírovali pri obidvoch drevinách pozdĺž vertikálneho profilu koruny. V prípade smreka sa zistili odlišné hodnoty plochy ihlíc a SLA medzi jednotlivými ročníkmi ihlíc. Na úrovni stromu mali buky oveľa viac biomasy drevných častí ako smreky, opačná situácia bola pri asimilačných orgánoch. Preto hodnoty podielu medzi biomasou asimilačných orgánov a celkovou biomasou stromu, ako aj pomeru medzi plochou asimilačných orgánov a celkovou biomasou stromu boli výrazne vyššie pri smreku než buku. Na úrovni porastu mala smrečina vyššie hodnoty indexu listovej plochy, t. j. LAI (18,64 m2.m−2 v porovnaní s bučinou (12,77 m2.m−2. Kým biomasa asimilačných orgánov bola 4,6-krát väčšia v smrekovom než v bukovom poraste, biomasa drevných časti bola porovnateľná v obidvoch porastoch. Tieto kontrasty naznačujú výrazne odlišnú rastovú stratégiu, resp. alokáciu biomasy medzi bučinami a smrečinami v mladých štádiách

  4. THE INFLUENCE OF FLOOR QUALITY ON DAIRY COW BEHAVIOUR AND THEIR BODY CLEANLINESS VPLYV KVALITY PODLAHY NA SPRÁVANIE DOJNÍC A ICH ČISTOTU POVRCHU TELA

    Directory of Open Access Journals (Sweden)

    Emília KUŠNÍROVÁ

    2012-03-01

    Full Text Available The aim of this study was to evaluate the influence of floor quality on the behaviour and cleanliness of the diary cow‘s body. The analysis results show that cows housed in the section with the separated manure solids spent more time lying (41.47% and also laid less in the corridor manure (0.91% than the cows in the section with separated manure solids and the rubber mats where they were laying generally less (35.71%, they spent more time lying in the corridor manure (1.55% and in terms of preference of lying area the cows clearly preferred the separated manure solids (25.8%. Based on observations we can say that cows housed in sections of slurry solid phase were cleaner (average 6.13 points than cows in the section with the rubber and a separated manure (averagely 7.28 points. Nowadays the free housing systems with different types of litter and boxes are frequently used, while the proper littering influences the lying preferences of an animal. In practice, for the dairy cows the deepened lying boxes littered with natural materials such as sawdust, shavings, straw, dried manure and sand boxes or elevated boxes covered with a synthetic material, mattresses or mats are used.Cieľom práce bolo zhodnotiť vplyv kvality podlahy na správanie a čistotu povrchu tela dojníc. Z analýzy výsledkov vyplýva, že dojnice ustajnené v sekcii so separovaným kalom hnojovice venovali ležaniu väčšiu časť (41,47% a ležali aj menej v hnojnej chodbe (0,91% ako dojnice v sekcii so separovaným kalom hnojovice aj gumenými matracmi, kde dojnice celkovo menej ležali (35,71%, viac času ležali v hnojnej chodbe (1,55% a z hľadiska uprednostňovania ležoviska ležali jednoznačne viac na separovanom kale hnojovice (25,8%. Na základe pozorovaní možno povedať, že dojnice ustajnené v sekciách so separovaným kalom hnojovice boli čistejšie (priemerne 6,13 bodov ako dojnice v sekcii so separovaným kalom hnojovice aj gumou (priemerne 7,28 bodov. V s

  5. Primjena spektrometrijskih metoda u analizi metalurških uzoraka

    Directory of Open Access Journals (Sweden)

    Anita Štrkalj

    2016-07-01

    Full Text Available Instrumentalne metode analize nezamjenjive su u znanstvenim istraživanjima, a sve više i u praksi gdje se zahtijeva što brže dobivanje rezultata, kao i što veća točnost. U metalurškoj praksi već se dulji niz godina primjenjuju spektrometrijske metode analize i to najčešće optička emisijska spektrometrija s izravnim očitanjem. Međutim koncentracija pojedinih legirnih elemenata u suvremenim metalnim materijalima vrlo je niska, često niža od granice detekcije optičkih emisijskih spektrometara s izravnim očitanjem. U ovom radu određivana je koncentracija bora u nodularnom lijevu primjenom optičke emisijske spektrometrije s izravnim očitanjem (OES, optičke emisijske spektrometrije s induktivno spregnutom plazmom (ICP-OES te atomske apsorpcijske spektrometrije s grafitnom tehnikom (AAS-GF. Dobiveni rezultati pokazali su da se optička emisijska spektrometrija s izravnim očitanjem i optička emisijska spektrometrija s induktivno spregnutom plazmom mogu primijeniti za određivanje bora u ispitivanim uzorcima (tablica 1. Ako se zahtijeva vrlo točna analiza, a posebno ako se radi o vrlo niskim koncentracijama, optička emisijska spektrometrija s induktivno spregnutom plazmom pogodnija je metoda, uz uvjet da su uzorci pravilno pripremljeni. Atomska apsorpcijska spektrometrija s grafitnom tehnikom nije pogodna za određivanje bora u uzorcima ovog tipa. Tijekom provođenja analize dodatna pažnja usmjerena je na eliminaciju negativnog utjecaja grafitne kivete na rezultat analize. Osim toga, u radu je praćen utjecaj načina otapanja uzoraka na rezultate analize bora spomenutim metodama. Rezultati su pokazali da pažljiva priprema uzoraka u zatvorenom sustavu pridonosi točnijem rezultatu analize.

  6. Afferent input selects NMDA receptor subtype to determine the persistency of hippocampal LTP in freely behaving mice

    Directory of Open Access Journals (Sweden)

    Jesús Javier Ballesteros

    2016-10-01

    Full Text Available The glutamatergic N-methyl-D-aspartate receptor (NMDAR is critically involved in many forms of hippocampus-dependent memory that may be enabled by synaptic plasticity. Behavioral studies with NMDAR antagonists and NMDAR subunit (GluN2 mutants revealed distinct contributions from GluN2A- and GluN2B-containing NMDARs to rapidly and slowly acquired memory performance. Furthermore, studies of synaptic plasticity, in genetically modified mice in vitro, suggest that GluN2A and GluN2B may contribute in different ways to the induction and longevity of synaptic plasticity. In contrast to the hippocampal slice preparation, in behaving mice, the afferent frequencies that induce synaptic plasticity are very restricted and specific. In fact, it is the stimulus pattern, and not variations in afferent frequency that determine the longevity of long-term potentiation (LTP. Here, we explored the contribution of GluN2A and GluN2B to LTP of differing magnitudes and persistencies in freely behaving mice. We applied differing high-frequency stimulation (HFS patterns at 100 Hz to the hippocampal CA1 region, to induce NMDAR-dependent LTP in wild-type (WT mice, that endured for 24h (late (L-LTP. In GluN2A-KO mice, E-LTP (HFS, 50 pulses was significantly reduced in magnitude and duration, whereas LTP (HFS, 2 x 50 pulses and L-LTP (HFS, 4 x 50 pulses were unaffected compared to responses in WT animals. By contrast, pharmacological antagonism of GluN2B in WT had no effect on E-LTP but significantly prevented LTP. E- LTP and LTP were significantly impaired by GluN2B antagonism in GluN2A-KO mice. These data indicate that the pattern of afferent stimulation is decisive for the recruitment of distinct GluN2A and GluN2B signaling pathways that in turn determine the persistency of hippocampal LTP. Whereas brief bursts of patterned stimulation preferentially recruit GluN2A and lead to weak and short-lived forms of LTP, prolonged, more intense, afferent activation recruits GluN2B

  7. Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos

    Energy Technology Data Exchange (ETDEWEB)

    Jenny, Matthew J. [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487 (United States); Aluru, Neelakanteswar [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States); Hahn, Mark E., E-mail: mhahn@whoi.edu [Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States)

    2012-10-15

    Although many drugs and environmental chemicals are teratogenic, the mechanisms by which most toxicants disrupt embryonic development are not well understood. MicroRNAs, single-stranded RNA molecules of ∼ 22 nt that regulate protein expression by inhibiting mRNA translation and promoting mRNA sequestration or degradation, are important regulators of a variety of cellular processes including embryonic development and cellular differentiation. Recent studies have demonstrated that exposure to xenobiotics can alter microRNA expression and contribute to the mechanisms by which environmental chemicals disrupt embryonic development. In this study we tested the hypothesis that developmental exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a well-known teratogen, alters microRNA expression during zebrafish development. We exposed zebrafish embryos to DMSO (0.1%) or TCDD (5 nM) for 1 h at 30 hours post fertilization (hpf) and measured microRNA expression using several methods at 36 and 60 hpf. TCDD caused strong induction of CYP1A at 36 hpf (62-fold) and 60 hpf (135-fold) as determined by real-time RT-PCR, verifying the effectiveness of the exposure. MicroRNA expression profiles were determined using microarrays (Agilent and Exiqon), next-generation sequencing (SOLiD), and real-time RT-PCR. The two microarray platforms yielded results that were similar but not identical; both showed significant changes in expression of miR-451, 23a, 23b, 24 and 27e at 60 hpf. Multiple analyses were performed on the SOLiD sequences yielding a total of 16 microRNAs as differentially expressed by TCDD in zebrafish embryos. However, miR-27e was the only microRNA to be identified as differentially expressed by all three methods (both microarrays, SOLiD sequencing, and real-time RT-PCR). These results suggest that TCDD exposure causes modest changes in expression of microRNAs, including some (miR-451, 23a, 23b, 24 and 27e) that are critical for hematopoiesis and cardiovascular

  8. Sergej Dovlatov: Kofer /S ruskog preveo Petar Karavlah

    Directory of Open Access Journals (Sweden)

    Petar Karavlah

    2012-12-01

    Full Text Available Kuja iz Ureda za vize rekla je: „Svaki emigrant ima pravo na tri kofera. Takva je norma. To su posebni propisi ministarstva.“Nije imalo smisla proturječiti. Ali ipak sam morao: „Samo tri kofera?! Što bih trebao sa stvarima?“„Na primjer?“„Na primjer, sa svojom zbirkom trkaćih auta?“„Prodajte je“, odsutno odgovori službenica. Zatim doda, lagano se namrštivši: „Ako nečim niste zadovoljni, podnesite žalbu.“„Zadovoljan sam.“Nakon zatvora bio sam svime zadovoljan.„Pa onda se i ponašajte tako.“Za tjedan dana već sam pakirao stvari. Ispostavilo se da mi je bio dovoljan jedan jedini kofer. Gotovo sam zaplakao od muke. Pa imao sam trideset i šest godina. Od toga sam osamnaest godina radio. Zaradio bih pa kupio nešto. Mislio sam da imam nekakvu imovinu. A na kraju – jedan kofer. I to prilično skromnih dimenzija. Ispada da sam siromah? Kako je do toga došlo?Knjige? Imao sam uglavnom zabranjene knjige. One nisu prolazile kroz carinu. Morao sam ih podijeliti poznanicima zajedno s takozvanim arhivom.

  9. Organizacija tehnološkog procesa remonta složenih tehničkih sistema u tehničkom remontnom zavodu u Čačku

    Directory of Open Access Journals (Sweden)

    Vojkan M. Radonjić

    2014-04-01

    Full Text Available Složeni tehnički sistemi (STS od ostalih tehničkih sistema (TS razlikuju se po konstrukciji i nameni, koja može biti: zaštita ljudi, materijalnih bogatstava i teritorije. Ako želimo da STS budu ispravni i imaju zadovoljavajući nivo pouzdanosti i gotovosti,  sistem remonta mora biti u stanju da podrži zahteve koji proističu iz njihove specifičnosti. Sistem njihovog remonta mora biti programiran, a upravljanje njime zasnovano na konceptu QMS (Quality management system. Organizacija remonta STS za potrebe sistema odbrane Republike Srbije, u Tehničkom remontnom zavodu u Čačku (TRZ, primer je organizacije u koju je implementiran  procesni model sistema upravljanja kvalitetom (QMS. Ovaj članak opisuje proces remonta STS u TRZ sa njegovim procesnim sistemom upravljanja kvalitetom (QMS, koji je preduslov za efikasan i efektivan proces remonta. Proces remonta prikazan je na primeru remonta guseničnog borbenog vozila. 

  10. Obligations Related to the Transportation of pet Animals (Eu Legislation / Povinnosti Súvisiace S Prepravou Spoločenských Zvierat (Európska Legislatíva

    Directory of Open Access Journals (Sweden)

    Pavlíková Barbara

    2015-06-01

    Full Text Available Prispevok sa zaobera otazkou, ktoru často riešia majitelia spoločenskych zvierat vo všetkych členskych štatoch EU. Poskytuje bližši pohľad na povinnosti spojene s cestovanim so zvieraťom medzi členskymi štatmi, alebo medzi členskym štatom a treťou - nečlenskou krajinou. Zameriava sa na nekomerčnu prepravu, ako je napriklad dovolenka, vylet a podobne. Praca prostrednictvom syntezy, analyzy a porovnania ponuka prehľad zakladnych legislativnych aktov v predmetnej oblasti. Venuje sa tiež otazke tzv. pet pasu, najdoležitejšieho identifikačneho dokladu, vyžadovaneho pre spoločenske zvierata na uzemi EU. Možno v nej najsť aj definicie najčastejšie použivanej terminologie.

  11. The saker falcon (Falco cherrug population, diet and nest boxes in Slovakia: LIFE-project report 2011–2014

    Directory of Open Access Journals (Sweden)

    Chavko Jozef

    2014-12-01

    Full Text Available Od októbra 201 0 do decembra 201 4 bol realizovaný projekt LIFE09 NAT/HU/000384 v štyroch Chránených vtácích územiach na Slovensku. Projekt nadviazal na predchádzajúci LIFE projekt, realizovaný v rokoch 2006 až 201 0. Oba projekty podporovali pravidelné sledovanie populácie sokola rároha a nové prístupy pre zachovanie druhu. Projekt LIFE09 prispel k zvýšeniu poznatkov o zložení potravy a rovnako aj o správaní sa tohto druhu. Populácia bola silne ovplyvnená v roku 201 3 mimoriadne zlými poveternostnými podmienkami pocasia (1 ,79 vyleteného mládata na zapocaté hniezdenie. Aj ked sa predpokladá, že celková populácia je vyššia, v roku 201 4 bolo zistených na Slovensku spolu 31 párov sokola rároha. Analýzou potravy (2991 jedincov, bolo identifikovaných 47 druhov koristi, z ktorých vtáky boli dominantné (80 %. Viac ako polovicu potravy tvoril holub domáci (Columba livia f. domestica (59 %

  12. Krizni menadžment u funkciji nove teorije menadžmenta

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    Jelena Legčević

    2014-10-01

    Full Text Available Današnji globalizacijski trendovi zahvatili su cijeli svijet i nemoguće je u njima ne sudjelovati. Svijet je u svim svojim pojavnostima postao uistinu međuovisan pa više nije moguće ignorirati novonastale promjene, a zbog kompleksnosti sustava teško je u njemu uspostaviti ravnotežu. Krize danas pogađaju gospodarske subjekte, političke i državne institucije, kao i različite druge organizacije, ali i čovjeka kao osobu. Krizni menadžment je bolji i učinkovitiji ako je krizna strategija određena pravovremeno. Svaka kriza ima svoj put za rješenje, ali je potrebno dobro razmotriti elemente koji ulaze u procjenu rizika s kojima se ulazi u krizu ili kriznu situaciju. Najbolji je način prevencije i obrane od krize osposobiti vlastito osoblje ili utemeljiti krizni stožer u kojemu će se nalaziti stručnjaci kojima su poznati svi alati i sva sredstva s pomoću kojih će se moći predvidjeti i riješiti kriza.

  13. Reducing urban diffuse pollution and surface water flooding using retrofit street trees

    Science.gov (United States)

    Rothwell, James; Stringer, Pete; Causer, Katherine; Ryan, Matt; Mangan, Steve; Appleton, Ian; Savage, Mike

    2016-04-01

    Nature-based solutions for the management of urban stormwater have been growing in popularity, but there is a lack of empirical performance data for field-scale installations, especially in a UK context. To address this deficiency, a novel retrofit street tree demonstration project was commissioned in the City of Salford, near Manchester (UK). Three fifteen year-old London Plane trees were planted within a large roadside tree trench on an urban residential street. The DeepRoot Silvia Cell modular suspended pavement system was used to maximise soil volume, avoid compaction and support large tree growth. Road runoff is directed to the tree trench via AKO Slot Kerbs. Water is then distributed evenly throughout the whole system via a perforated pipe. Excess water is conveyed out of the system via an underdrain, which is subsequently connected to the sewer network. The tree trench is lined with an impermeable membrane. Access chambers are positioned on the inflow and outflow of the tree trench to facilitate hydrological and water quality monitoring. Installation was completed in autumn 2015 and monitoring will be conducted over a three year period. This paper will provide an overview of the installation process and present initial results on the pollutant removal performance and hydrological functioning of the system.

  14. Úloha UNASUR v latinskoamerických integračných procesoch na začiatku 21. storočia

    OpenAIRE

    Marián Šabo

    2013-01-01

    Jedným z najnovších a najambicióznejších integračných zoskupení v Latinskej Amerike je Únia juhoamerických národov (UNASUR), vytvorená v roku 2008 na podporu regionálnej integrácie v otázkach demokracie, vzdelávania, energetiky, životného prostredia, infraštruktúry a bezpečnosti a so zámerom odstrániť sociálnu nerovnosť a vylúčenie. Vytvorená je podľa vzoru Európskej únie a rovnako ako EÚ má UNASUR veľmi rozsiahle a ambiciózne ciele. Hlavným cieľom UNASUR je politická, ekonomická, sociálna a ...

  15. 2011 Einstein Fellows Chosen

    Science.gov (United States)

    2011-03-01

    ASA has announced the selection of the 2011 Einstein Fellows who will conduct research related to NASA's Physics of the Cosmos program, which aims to expand our knowledge of the origin, evolution, and fate of the Universe. The Einstein Fellowship provides support to the awardees for three years, and the Fellows may pursue their research at a host university or research center of their choosing in the United States. The new Fellows will begin their programs in the fall of 2011. The new Einstein Fellows and their host institutions are listed below: * Akos Bogdan (Smithsonian Astrophysical Observatory, Cambridge, Mass.) * Samuel Gralla (University of Maryland, College Park, Md.) * Philip Hopkins (University of California at Berkeley) * Matthew Kunz (Princeton University, Princeton, N.J.) * Laura Lopez (Massachusetts Institute of Technology, Cambridge, Mass.) * Amy Reines (National Radio Astronomy Observatory, Charlottesville, Virg.) * Rubens Reis (University of Michigan, Ann Arbor) * Ken Shen (Lawrence Berkeley National Laboratory, Berkeley, Calif.) * Jennifer Siegal-Gaskins (California Institute of Technology, Pasadena) * Lorenzo Sironi (Harvard University, Cambridge, Mass.) NASA has two other astrophysics theme-based fellowship programs: the Sagan Fellowship Program, which supports research into exoplanet exploration, and the Hubble Fellowship Program, which supports research into cosmic origins. More information on the Einstein Fellowships can be found at: http://cxc.harvard.edu/fellows/

  16. Řídicí modul BLDC motoru

    OpenAIRE

    2016-01-01

    Diplomová práca sa zaoberá návrhom a realizáciou hardvéru a softvéru pre riadenie a reguláciu vysokootáčkového pohonu s BLDC motorom, ktorý bude slúžiť ako vreteno pre CNC frézovačku. V práci je podrobne popísaný návrh schémy zapojenia a dosky plošných spojov silovej časti, riadiacej časti a napájacej časti trojfázového tranzistorového meniča riadeného DSP procesorom. Taktiež je podrobne popísaný program DSP procesora pre riadenie a reguláciu BLDC motora, ktorého funkcia je overovaná pomocou ...

  17. Resposta taquicárdica e controle autonômico no exercício físico em modelo genético de insuficiência cardíaca Tachycardic response and autonomic control in physical exercise in genetic model of cardiac insufficiency

    Directory of Open Access Journals (Sweden)

    Telma F. Cunha

    2009-08-01

    Full Text Available O aumento da atividade nervosa simpática e a taquicardia em repouso ou durante esforços físicos estão associados ao aumento da morbimortalidade, mesmo na ausência de sinais clínicos de doença cardíaca. Sabendo-se da importância dos receptores α2A/α2C-adrenérgicos na modulação da atividade nervosa e frequência cardíaca (FC, o presente trabalho utiliza um modelo genético de cardiomiopatia induzida por excesso de catecolaminas circulantes baseado na inativação gênica dos receptores α2A/α2C-adrenérgicos em camundongos (α2A/α2CKO para verificar a resposta da FC ao exercício físico (EF, assim como o controle simpatovagal da FC ao EF. Testou-se a hipótese de que haveria resposta taquicárdica exacerbada durante o EF nos camundongos α2A/α2CKO mesmo quando a função cardíaca ainda estivesse preservada em repouso, sendo o receptor α2A-adrenérgico o principal responsável por essa resposta. Camundongos machos da linhagem C57Bl6J, controle (CO e com inativação gênica para os receptores α2A (α2AKO, α2C α2CKO e α2A/α2CKO foram submetidos a um teste de tolerância ao esforço físico. Outros dois grupos de camundongos, CO e α2A/α2CKO, foram submetidos ao bloqueio farmacológico dos receptores muscarínicos e β-adrenérgicos e ao EF progressivo para se avaliar a contribuição simpatovagal para a taquicardia de EF. Observou-se intolerância ao esforço físico (1.220 ± 18 e 1.460 ± 34 vs. 2.630 ± 42m, respectivamente e maior taquicardia ao EF (765 ± 16 e 792 ± 13 vs. 603 ± 18bpm, respectivamente nos camundongos α2AKO e α2A/α2CKO vs. CO. Além disso, o balanço autonômico estava alterado nos camundongos α2A/α2CKO pela hiperatividade simpática e menor efeito vagal cardíaco. Esses resultados demonstram a importância dos receptores α2A/α2C-adrenérgicos no controle autonômico não só no repouso, mas também durante o EF, sendo o receptor α2A-adrenérgico o responsável pela hiperatividade simp

  18. Produkce vodíku s využitím bakterií rodu Clostridium

    OpenAIRE

    2013-01-01

    Bakalárska práca sa zaoberá produkciou vodíka baktériami rodu Clostridium – konkrétne C. butyricum a C. tyrobutyricum. V teoretickej časti sú rozobrané spôsoby výroby vodíka, rozdelené na biologické a nebiologické metódy. Práca sa ďalej zameriava na praktické využitie vyššie uvedených mikroorganizmov na účely výroby vodíku. Ako jedno z možných rastových médií pre vyššie uvedené baktérie predstavuje srvátku z mliekarenských výrob. V experimentálnej časti práce bola použitá polymerázová reťazov...

  19. Mikroprogram pre riadenie procesu spájania operandov v architektúre DF KPI

    Directory of Open Access Journals (Sweden)

    Norbert Ádám

    2013-12-01

    Full Text Available V článku uvedená architektúra je založená na výpočtovom modeli „data flow“, v ktorom riadenie výpočtu je založené na spracovaní toku dát. Základným prvkom DF architektúry je koordinačný procesor (CP, ktorý je zodpovedný za riadenie a organizáciu vykonávania inštrukcií. Štruktúrna organizácia CP je navrhnutá ako dynamický multifunkčný systém. Pri vykonávaní operácií môže CP prechádzať rôznymi stavmi, v dôsledku čoho táto jednotka predstavuje dynamický prúdový systém. Prechod a poradie prechodu medzi jednotlivými stavmi CP je podmienený typom spracovaného operátora pri interpretovaní prúdu operandov. Z možných typov operátorov, článok uvádza mikroprogramové riadenie pre jednovstupové operátory.

  20. Identification and Pharmacological Analysis of High Efficacy Small Molecule Inhibitors of EGF-EGFR Interactions in Clinical Treatment of Non-Small Cell Lung Carcinoma: a Computational Approach.

    Science.gov (United States)

    Gudala, Suresh; Khan, Uzma; Kanungo, Niteesh; Bandaru, Srinivas; Hussain, Tajamul; Parihar, Ms; Nayarisseri, Anuraj; Mundluru, Hema Prasad

    2015-01-01

    Inhibition of EGFR-EGF interactions forms an important therapeutic rationale in treatment of non-small cell lung carcinoma. Established inhibitors have been successful in reducing proliferative processes observed in NSCLC, however patients suffer serious side effects. Considering the narrow therapeutic window of present EGFR inhibitors, the present study centred on identifying high efficacy EGFR inhibitors through structure based virtual screening strategies. Established inhibitors - Afatinib, Dacomitinib, Erlotinib, Lapatinib, Rociletinib formed parent compounds to retrieve similar compounds by linear fingerprint based tanimoto search with a threshold of 90%. The compounds (parents and respective similars) were docked at the EGF binding cleft of EGFR. Patch dock supervised protein-protein interactions were established between EGF and ligand (query and similar) bound and free states of EGFR. Compounds ADS103317, AKOS024836912, AGN-PC-0MXVWT, GNF-Pf-3539, SCHEMBL15205939 were retrieved respectively similar to Afatinib, Dacomitinib, Erlotinib, Lapatinib, Rociletinib. Compound- AGN-PC-0MXVWT akin to Erlotinib showed highest affinity against EGFR amongst all the compounds (parent and similar) assessed in the study. Further, AGN-PC-0MXVWT brought about significant blocking of EGFR-EGF interactions in addition showed appreciable ADMET properties and pharmacophoric features. In the study, we report AGN-PC-0MXVWT to be an efficient and high efficacy inhibitor of EGFR-EGF interactions identified through computational approaches.

  1. Transport, metabolism, and endosomal trafficking-dependent regulation of intestinal fructose absorption.

    Science.gov (United States)

    Patel, Chirag; Douard, Veronique; Yu, Shiyan; Gao, Nan; Ferraris, Ronaldo P

    2015-09-01

    Dietary fructose that is linked to metabolic abnormalities can up-regulate its own absorption, but the underlying regulatory mechanisms are not known. We hypothesized that glucose transporter (GLUT) protein, member 5 (GLUT5) is the primary fructose transporter and that fructose absorption via GLUT5, metabolism via ketohexokinase (KHK), as well as GLUT5 trafficking to the apical membrane via the Ras-related protein-in-brain 11 (Rab11)a-dependent endosomes are each required for regulation. Introducing fructose but not lysine and glucose solutions into the lumen increased by 2- to 10-fold the heterogeneous nuclear RNA, mRNA, protein, and activity levels of GLUT5 in adult wild-type mice consuming chow. Levels of GLUT5 were >100-fold that of candidate apical fructose transporters GLUTs 7, 8, and 12 whose expression, and that of GLUT 2 and the sodium-dependent glucose transporter protein 1 (SGLT1), was not regulated by luminal fructose. GLUT5-knockout (KO) mice exhibited no facilitative fructose transport and no compensatory increases in activity and expression of SGLT1 and other GLUTs. Fructose could not up-regulate GLUT5 in GLUT5-KO, KHK-KO, and intestinal epithelial cell-specific Rab11a-KO mice. The fructose-specific metabolite glyceraldehyde did not increase GLUT5 expression. GLUT5 is the primary transporter responsible for facilitative absorption of fructose, and its regulation specifically requires fructose uptake and metabolism and normal GLUT5 trafficking to the apical membrane.

  2. Založení internetové cestovní agentury

    OpenAIRE

    Solárik, Róbert

    2011-01-01

    Táto diplomová práca sa zameriava na vytvorenie podnikateľského zámeru pre online cestovnú agentúru s cieľom zistiť, či je ekonomicky výhodné začať podnikať v turistickom ruchu ako predajca zájazdov cez internet. Táto práca tiež vykonáva analýzy okolia a podáva odporučenia vo forme časového harmonogramu, marketingového plánu, operačného plánu a finančného plánu. Na základe predpokladaných finančných výkazov hodnotí ekonomickú výhodnosť projektu. This dissertation thesis focuses on creating...

  3. Progresivní využití neuronových sítí v telekomunikacích

    OpenAIRE

    2009-01-01

    Ciežom tento prace bolo zoznámiť sa so sieťovými prvkami konvergovaných sieti. V dnes dobe sa kladie na sieťové prvky vežký dôraz preto je dôležité ako bude riešená priorita. V teoretická časť sa zaobera sieťovými prvkami a neurónovými sieťami. Neurónové siete boli rozoberané od vzniku až po súčastnosť. Úlohou bolo navrhnúť topologiu neurónovej, ktorá by dokázala riadiť sieťový prvok. Jedná sa o trojvrstvovú preceptrónovú sieť so štyrmy vstupmy a jedným výstupom. Simulácia bola uskutočnená v ...

  4. Planetary Systems and the Origins of Life

    Science.gov (United States)

    Pudritz, Ralph; Higgs, Paul; Stone, Jonathon

    2013-01-01

    Preface; Part I. Planetary Systems and the Origins of Life: 1. Observations of extrasolar planetary systems Shay Zucker; 2. The atmospheres of extrasolar planets L. Jeremy Richardson and Sara Seager; 3. Terrestrial planet formation Edward Thommes; 4. Protoplanetary disks, amino acids and the genetic code Paul Higgs and Ralph Pudritz; 5. Emergent phenomena in biology: the origin of cellular life David Deamer; Part II. Life on Earth: 6. Extremophiles: defining the envelope for the search for life in the Universe Lynn Rothschild; 7. Hyperthermophilic life on Earth - and on Mars? Karl Stetter; 8. Phylogenomics: how far back in the past can we go? Henner Brinkmann, Denis Baurain and Hervé Philippe; 9. Horizontal gene transfer, gene histories and the root of the tree of life Olga Zhaxybayeva and J. Peter Gogarten; 10. Evolutionary innovation versus ecological incumbency Adolf Seilacher; 11. Gradual origins for the Metazoans Alexandra Pontefract and Jonathan Stone; Part III. Life in the Solar System?: 12. The search for life on Mars Chris McKay; 13. Life in the dark dune spots of Mars: a testable hypothesis Eörs Szathmary, Tibor Ganti, Tamas Pocs, Andras Horvath, Akos Kereszturi, Szaniszlo Berzci and Andras Sik; 14. Titan: a new astrobiological vision from the Cassini-Huygens data François Raulin; 15. Europa, the Ocean Moon: tides, permeable ice, and life Richard Greenberg; Index.

  5. Termoplastické kompozity pro automobilové aplikace

    OpenAIRE

    Zbončák, Marek

    2014-01-01

    Diplomová práca sa zaoberá prípravou teromplastických kompzoitov s PMMA a PC matricou s potenciálnym využitím v automobilovom priemysle. Ako výstuž boli použité krátke sklenené, uhlíkové a PBO (poly(p-fenylén benzobisoxazol)) vlákna známe pod obchodným názvom Zylon®. Práve do PBO vlákien boli vkladané veľké nádeje vzhľadom na ich ohromujúce mechanické vlastnosti. Vplyv objemového zlomku vlákien na modulu pružnosti, pevnosť a ťažnosť kompozitov bol skúmaný. Experimentálne zistený modul pružnos...

  6. "Trojitá hra" - FTTH

    OpenAIRE

    Bobkovič, Peter

    2008-01-01

    Tento projekt sa zaoberá možnosťami realizácie pripojenia poslednej míle k užívatežovi pomocou FTTH, čo je optika až do domu. Celá práca je rozdelená na 2 časti a to všeobecný prehžad FTTH a vlastné meranie. Vo všeobecnom prehžade sú postupne uvedené druhy sietí FTTH, problematika s FTTH spojená, zmapovaná situácia vo svete a trojitá hra (cenník, ponuka TV a pod.). Vo druhej časti je vypracované postupné zmeranie a výsledky troch vykonaných meraní (PPM, OTDR, priama metóda) ako aj vzhžad čist...

  7. Empowering student leaders to nurture the first year experience through cross-cultural diversity. A Practice Report Nāu te raurau, Nāku te raurau, ka ora ai te iwi

    Directory of Open Access Journals (Sweden)

    Deborah Laurs

    2013-08-01

    Full Text Available Victoria University offers a number of peer-support and mentoring programs for first-year students, including Te Pūtahi Atawahi (mentoring and holistic support for Māori and Pasifika students, PASS (Peer Assisted Study Support and Campus Coaches (Orientation Week guides. In the past, despite having similar goals of providing peer-support to foster a sense of community, such programs have operated in isolation. However, a recent initiative has seen the development of a core leadership training module, jointly designed and delivered by staff from Te Pūtahi Atawhai and Student Learning Support. Seeking to equip student-leaders with an understanding of some of the holistic Māori values, Kotahitanga (unity, Ako (teaching and learning, Manaakitanga (empathy/hospitality, Whakamana (respect, Whakanui (acknowledging success, Whakawhanaungatanga (building strong networks and Rangatiratanga (ability to bring groups together/Self-direction, this core training offers the potential to strengthen Treaty of Waitangi relationships and nurture inter-cultural awareness, developing a pan-university sense of belongingness in the process. 

  8. Genomic research for important pathogenic bacteria in China

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of point.We can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug targets.The newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease.

  9. Individual Genome of the Russian Male: SNP Calling and a de novo Assembly of Unmapped Reads.

    Science.gov (United States)

    Chekanov, N N; Boulygina, E S; Beletskiy, A V; Prokhortchouk, E B; Skryabin, K G

    2010-07-01

    A somatic cell genome was recently resequenced for a patient with renal cancer. The data were submitted to the NCBI Sequence Read Archive under the accession number SRA012240. Here, we have performed SNP calling for the genome and compared it with several published genomes. We have found 2, 921, 724 SNPs, including 1, 472, 679 newly described ones. Among them, 63, 462 SNPs have been mapped to the Y chromosome and, based on 18 markers, the genome has been ascribed to the R1a1a haplogroup predominant in Russian males. The mitochondrial haplogroup has been determined as U5a, which is also common in the European part of Russia. Short reads unmapped to the human genome were used for thede novoassembly of DNA sequences. This resulted in genome-specific contigs (more than 100 bp in length) with an overall length of 154 kbp (for GAII) and 4.7 kbp (for SOLiD).

  10. Adverse Effect of H2O2 Change on Morphology, Mitochondrial Membrane Permeability and Antioxidant Enzyme in Root of Dianthus Chinensis L. under Salt Stress

    Directory of Open Access Journals (Sweden)

    Xue-qin He

    2013-04-01

    Full Text Available Dianthus Chinensis L. is a salt-tolerant ornamental plant. Root is the first and critical part of plant to encounter soli salinity. In order to elucidate H2O2 impact on root morphology and mitochondrial permeability transition as well as activities of antioxidant enzymes in root ofDianthus Chinensis L., we treated seedling with H2O2 and DMTU under NaCl. The results revealed that change of H2O2 level under NaCl would negatively influence the root growth, as well as lower the value of mitochodrial membrane absorbance at 540 nm and the ratio of Cyt c/a. Meanwhile, SOD and POD under NaCl plus H2O2 and NaCl plus DMTU were far lower than those under NaCl alone.

  11. The reversal of the Sun's magnetic field in cycle 24

    CERN Document Server

    Mordvinov, Alexander V; Bertello, Luca; Petrie, Gordon J D

    2016-01-01

    Analysis of synoptic data from the Vector Stokes Magnetograph (VSM) of the Synoptic Optical Long-term Investigations of the Sun (SOLIS) and the NASA/NSO Spectromagnetograph (SPM) at the NSO/Kitt Peak Vacuum Telescope facility shows that the reversals of solar polar magnetic fields exhibit elements of a stochastic process, which may include the development of specific patterns of emerging magnetic flux, and the asymmetry in activity between northern and southern hemispheres. The presence of such irregularities makes the modeling and prediction of polar field reversals extremely hard if possible. In a classical model of solar activity cycle, the unipolar magnetic regions (UMRs) of predominantly following polarity fields are transported polewards due to meridional flows and diffusion. The UMRs gradually cancel out the polar magnetic field of the previous cycle, and re-build the polar field of opposite polarity setting the stage for the next cycle. We show, however, that this deterministic picture can be easily a...

  12. Fare astronomia con piccoli telescopi

    CERN Document Server

    Gainer, Michael K

    2007-01-01

    Non sono necessariamente richiesti strumenti mastodontici per produrre risultati scientificamente validi nel campo dell’astronomia. Anche l’astrofilo dotato di un piccolo telescopio, con un diametro di soli 8-9 cm, può contribuire alla scienza del cielo realizzando utili osservazioni del Sole, della Luna, dei pianeti, delle comete, degli asteroidi, delle stelle doppie o variabili, delle nebulose e degli ammassi stellari. Il manuale di M.K. Gainer spiega quale sia la dotazione minima (un piccolo telescopio, un computer, una semplice fotocamera digitale), come utilizzarla, e quali siano le tecniche appropriate da adottare nelle osservazioni. Offre inoltre schemi per interpretare e ridurre i dati raccolti, nonché schede da compilare e da spedire ai centri di raccolta internazionali. Questo libro è il passaporto grazie al quale l’astrofilo può entrare a pieno titolo nel mondo affascinante della scienza astronomica.

  13. Reseñas bibliográficas

    OpenAIRE

    Romero-Pérez, Jorge Enrique

    2014-01-01

    Se presentan las siguientes reseñas bibliográficas:        Sánchez González, Álvaro: El Foro [Revista] (No.13, 2012)/ Reseñado por J. Enrique Romero P.         Solis, Alex: La Constitución es lo que los jueces dicen (2009)/ Reseñado por J. Enrique Romero P.         Salas, Minor: Los rostros de la justicia penal. Ensayos críticos sobre temas fundamentales del Derecho procesal Penal (2012)/ Reseñado por J. Enrique Romero P.         Llobet Rodríguez, Javier: Derecho Humanos en la justicia penal,...

  14. Vector Nonlinear Schr\\"odinger Equation on the half-line

    CERN Document Server

    Caudrelier, V

    2011-01-01

    We investigate the Manakov model or, more generally, the vector nonlinear Schr\\"odinger equation on the half-line. Using a B\\"acklund transformation method, two classes of integrable boundary conditions are derived: mixed Neumann/Dirichlet and Robin boundary conditions. Integrability is shown by constructing a generating function for the conserved quantities. We apply a nonlinear mirror image technique to construct the inverse scattering method with these boundary conditions. The important feature in the reconstruction formula for the fields is the symmetry property of the scattering data emerging from the presence of the boundary. Particular attention is paid to the discrete spectrum. An interesting phenomenon of transmission between the components of a vector soliton interacting with the boundary is demonstrated. This is specific to the vector nature of the model and is absent in the scalar case. For one-soliton solutions, we show that the boundary can be used to make certain components of the incoming soli...

  15. El Hombre, un ser integral

    Directory of Open Access Journals (Sweden)

    Gabriel Alexander Solórzano

    2008-12-01

    Full Text Available El artículo pretende mostrar la dificultad que existe al momento de elaborar una precisa definición del hombre como ser integral, para ello se recurre a las nociones esenciales de la antropología filosófica, la nueva concepción del giro cristia- no de la fenomenología francesa, se mira grosso modo la visión personalista de Juan Pablo II sobre el hombre y la visión integradora de Panikkar del humanismo cosmote- ándrico, por último se encuentra la visión del humanismo cristiano y el desarrollo soli- dario enfatizado en el Magisterio de la Iglesia Católica como intento de respuesta a la crucial pregunta sobre el hombre y su mundo relacional. Palabras clave: Filosofía, Teología moral, Fenomenología, Humanismo, Perso- nalismo, Cosmoteándrico. 

  16. Evaluation of a method to measure water content in porous media by employing ultrasound

    Directory of Open Access Journals (Sweden)

    Luis Leonardo Sáenz Cruz

    2010-06-01

    Full Text Available A method to measure water content in porous media, such as solis and grains, was developed as a real time nondestructive test. The method was based on piezoelectric ultrasonic transducers as a sensor system. Transmiters and receivers was developed to administrate the sensors system and ultrasonic signal. Transmiters and receivers are placed facing each other and located inside the porous media 10 cm apart. The method was evaluated in two porous meda, namely a column 30 cm coarse sand and a paddy rice variety Fedearroz 50, in order to evaluate the sensors system performance in two different porous media with different water holder capacity. Tools were developed for data acquisition, capacity of 16 analog signal, 12 bits resolution. Electronic circuits, C++ OPP programming and Matlab were used. The results showed a monotonically increment of millivolts as a response of the transducer as the water content was decreasing

  17. Simulation of Flow Characteristic and Structural optimization of Shell and Tube Heat Exchanges%管壳式换热器流动特性仿真模拟及结构优化

    Institute of Scientific and Technical Information of China (English)

    周伟; 王庆明

    2009-01-01

    运用SoliWorks中的流体仿真模拟插件Cosmosfloworks对管壳式换热器的流动特性进行仿真模拟,对管壳式换热器的管束腐蚀情况进行研究.首先通过sofidWorks对管壳式换热器进行实体建模,然后结合流体仿真模拟插件Cosmosfloworks完成流体仿真计算,最后得出导致腐蚀的原因并提出管壳式换热器的结构优化方案.

  18. C. elegans behavior of preference choice on bacterial food.

    Science.gov (United States)

    Abada, Emad Abd-elmoniem; Sung, Hyun; Dwivedi, Meenakshi; Park, Byung-Jae; Lee, Sun-Kyung; Ahnn, Joohong

    2009-09-01

    Caenorhabditis elegans is a free living soil nematode and thus in its natural habitat, C. elegans encounters many different species of soil bacteria. Although some soil bacteria may be excellent sources of nutrition for the worm, others may be pathogenic. Thus, we undertook a study to understand how C. elegans can identify their preferred food using a simple behavioral assay. We found that there are various species of soil bacteria that C. elegans prefers in comparison to the standard laboratory E. coli strain OP50. In particular, two bacterial strains, Bacillus mycoides and Bacillus soli, were preferred strains. Interestingly, the sole feeding of these bacteria to wild type animals results in extended lifespan through the activation of the autophagic process. Further studies will be required to understand the precise mechanism controlling the behavior of identification and selection of food in C. elegans.

  19. La implementación de las políticas públicas para el sector solidario en Ecuador, Perú y Colombia periodo 2004 a 2014

    OpenAIRE

    Rodríguez Rangel, Javier Gilberto

    2015-01-01

    En este trabajo se analiza específicamente la implementación de las políticas públicas sobre un conjunto de grupos y actividades económicas que crecen y se desarrollan como opción de sobrevivencia y renta para sectores sociales generalmente marginalizados del proceso competitivo característico de la dinámica de mercado capitalista y marginalizados también de políticas públicas de empleo y profesionalización, para países de la Región Andina (Ecuador, Perú y Colombia). La Economía Social y Soli...

  20. Specefekti – to lietojums latviešu un ārzemju kino

    OpenAIRE

    Balders, Jānis

    2013-01-01

    Darbā apskatītā tēma ir: ”Specefekti – to lietojums latviešu un ārzemju kino” Darbā apskatīti specefekti Latviešu un ārzemju kino, kā arī aprakstīta specefektu izveide soli pa solim. Par pētniecības problēmjautājumu tika izvirzīts jautājums, kādi specefekti tiek izmantoti latviešu kino un vai tie spēj konkurēt ar ārzemju filmās redzamajiem specefektiem. Darbā apkopota teorētiskā literatūra par kino, specefektiem un to vēsturi, un metodoloģija, kas ietver nestrukturēto interviju, specefektu...

  1. Effect of uncertainties in solar synoptic magnetic flux maps in modeling of solar wind

    Science.gov (United States)

    Pevtsov, Alexei A.; Bertello, Luca; MacNeice, Peter

    2015-12-01

    Recently, the NSO/SOLIS team developed variance (error) maps that represent uncertainties in magnetic flux synoptic charts. These uncertainties are determined by the spatial variances of the magnetic flux distribution from full disk magnetograms that contribute to each bin in the synoptic chart. Here we present a study of the effects of variances on solar wind parameters (wind speed, density, magnetic field, and temperature) derived using the WSA-ENLIL model and ensemble modeling approach. We compare the results of the modeling with near-Earth solar wind magnetic field and plasma data as extracted from NASA/GSFC's OMNI data set. We show that analysis of uncertainties may be useful for understanding the sensitivity of the model predictions to short-term evolution of magnetic field and noise in the synoptic magnetograms.

  2. París en el siglo XX según Julio Verne

    OpenAIRE

    Diego, Rosa de

    1995-01-01

    L'objet de cet article est l'analyse du roman de Jules Verne "Paris au XX siècle", écrit en 1863 et inédit jusqu'a 1994. Il s'agit d'un roman d'imagination scientifique parce qu'il décrit et anticipe, avec une exactitude surprenante, comment sera Paris pendant la deuxieme moitié du XX siècle, sous un point de vue urbanistique, technologique, économique et social. Jules Verne apparait également comme un écrivain philosophe, avec une vision péssimiste du progrès de la civilisation et de la soli...

  3. An evaluation of Bradfordizing effects

    CERN Document Server

    Mayr, Philipp

    2008-01-01

    The purpose of this paper is to apply and evaluate the bibliometric method Bradfordizing for information retrieval (IR) experiments. Bradfordizing is used for generating core document sets for subject-specific questions and to reorder result sets from distributed searches. The method will be applied and tested in a controlled scenario of scientific literature databases from social and political sciences, economics, psychology and medical science (SOLIS, SoLit, USB Koeln Opac, CSA Sociological Abstracts, World Affairs Online, Psyndex and Medline) and 164 standardized topics. An evaluation of the method and its effects is carried out in two laboratory-based information retrieval experiments (CLEF and KoMoHe) using a controlled document corpus and human relevance assessments. The results show that Bradfordizing is a very robust method for re-ranking the main document types (journal articles and monographs) in today's digital libraries (DL). The IR tests show that relevance distributions after re-ranking improve ...

  4. Life history and systematics of Albusambia elaphoglossumae (Lepidoptera: Crambidae): a new genus and species of musotimine with leaf-mining biology from Costa Rica.

    Science.gov (United States)

    Solis, M Alma; Davis, Donald R; Nishida, Kenji

    2005-01-01

    Albusambia elaphoglossumae Solis & Davis, a new genus and species, is described. It was discovered mining the fronds of the fern Elaphoglossum conspersum in Costa Rica (San José and Cartago Provinces, at elevations of 2300-3100 m). The type series was obtained by rearing of the immature stages in laboratory. The adult is defined by unique genital characters, and the pupa with a medial depression on the vertex and with two anterolateral horn-like structures on the prothorax. The larva is a gregarious leaf miner with its body flattened dorsoventrally and head prognathous; morphological adaptations to its leaf-mining habit are new to the Musotiminae. Fern-feeding musotimines are important to the discovery of new biological control agents for invasive ferns.

  5. Fazna ravnoteža alkalijskih nitrata (NaNO3, KNO3, RbNO3, CsNO3) u sustavu glicerol + H2O pri različitim temperaturama

    OpenAIRE

    Zhang, H.-Y.; Li, S.-N.; Zhai, Q.-G.; Ou, Y.-M.; Jiang, Y.-C.; Hu, M.-C.

    2014-01-01

    Uređajem vlastite izrade mjerena je topljivost alkalijskih nitrata (NaNO3/KNO3/RbNO3/CsNO3) u smjesi otapala glicerol [HOCH2CH(OH)CH2OH] + H2O u ovisnosti o masenom udjelu glicerola (0,0 – 1,0) pri 35 i 45 °C. Određene su gustoće i indeksi loma zasićenih otopina NaNO3/KNO3/ RbNO3/CsNO3 + smjesa otapala glicerol + H2O. Eksperimentalni rezultati su pokazali da se s povećanjem masenog udjela glicerola u svim sustavima smanjuju topljivost soli i gustoća otopine, dok se indeks loma postupno poveća...

  6. Study of organic N transformation in red soils by 15N tracer method

    Institute of Scientific and Technical Information of China (English)

    YeQing-Fu; ZhangQin-Zheng; 等

    1997-01-01

    Uniformly 15N-labelled ryegrass was used to investigate NH4+-production,microbial transformation and humification of organic N in two types of red soils by incubating the soils amended with labelled material.The results showed that there was little significant difference in biomass N transformation in the tested solis between 15N tracer method and conventional method,but the amount of NH4++-N released form the ryegrass in the clayey soil than in the sandy soil at all sampling time .By 120d of incubation,humified N was less than 10% of the amount of the applied N in two types of red soils and the amount of residual N in the clayey red soil was obviously higher than that in the sandy red soil.

  7. Bisector Area: A New Metric for Calculating Spectral Line Bisectors

    Science.gov (United States)

    Evans, Kaitlin; Harker, Brian

    2016-05-01

    We present a study of a new metric for characterizing spectral line bisectors from integrated Sun-as-a-star measurements of photospheric spectral lines. This metric, which we call bisector area, differs from previous analysis methods in that it characterizes the entire bisector (up to a maximum intensity limit) with a single scalar quantity. In this preliminary study, we analyzed data from the Synoptic Optical Long-term Investigations of the Sun Integrated Sunlight Spectrometer (SOLIS/ISS) during the decline and rise of solar cycles 23 and 24, respectively, to diagnose any potential correlations between bisector area and other known changes in spectral line properties as a function of time. This work was carried out through the National Solar Observatory Research Experiences for Undergraduates (REU) Program, which is funded by the National Science Foundation (NSF). The National Solar Observatory is operated by the Association of Universities for Research in Astronomy, Inc. (AURA) under cooperative agreement with the NSF.

  8. What if we had a magnetograph at Lagrangian L5?

    Science.gov (United States)

    Pevtsov, Alexei A.; Bertello, Luca; MacNeice, Peter; Petrie, Gordon

    2016-11-01

    Synoptic Carrington charts of magnetic field are routinely used as an input for modelings of solar wind and other aspects of space weather forecast. However, these maps are constructed using only the observations from the solar hemisphere facing Earth. The evolution of magnetic flux on the "farside" of the Sun, which may affect the topology of coronal field in the "nearside," is largely ignored. It is commonly accepted that placing a magnetograph in Lagrangian L5 point would improve the space weather forecast. However, the quantitative estimates of anticipated improvements have been lacking. We use longitudinal magnetograms from the Synoptic Optical Long-term Investigations of the Sun (SOLIS) to investigate how adding data from L5 point would affect the outcome of two major models used in space weather forecast.

  9. 测序新技术Ion Torrent PGM及其法医学应用%The new sequencing technology-Ion Torrent PGM and its forensic application

    Institute of Scientific and Technical Information of China (English)

    刘宇轩; 张文琼; 黄代新

    2015-01-01

    目前,基因测序技术发展迅速,继Roche公司的454系统、Illumina公司的HiSeq 2000系统和AB公司的 SOLiD系统之后,Life Technologies公司于2010年推出了Ion Torrent Personal G enome Machine (PGM)测序仪.Ion Torrent是基于半导体芯片的测序技术,不属于核酸标记、荧光检测的生化技术.与其他测序技术相比,操作简便,费用较低,具有强大的扩展性.本文就Ion Torrent PGM的特点及其在法医学中的应用进行简要介绍.

  10. A GPU-Computing Approach to Solar Stokes Profile Inversion

    CERN Document Server

    Harker, Brian J

    2012-01-01

    We present a new computational approach to the inversion of solar photospheric Stokes polarization profiles, under the Milne-Eddington model, for vector magnetography. Our code, named GENESIS (GENEtic Stokes Inversion Strategy), employs multi-threaded parallel-processing techniques to harness the computing power of graphics processing units GPUs, along with algorithms designed to exploit the inherent parallelism of the Stokes inversion problem. Using a genetic algorithm (GA) engineered specifically for use with a GPU, we produce full-disc maps of the photospheric vector magnetic field from polarized spectral line observations recorded by the Synoptic Optical Long-term Investigations of the Sun (SOLIS) Vector Spectromagnetograph (VSM) instrument. We show the advantages of pairing a population-parallel genetic algorithm with data-parallel GPU-computing techniques, and present an overview of the Stokes inversion problem, including a description of our adaptation to the GPU-computing paradigm. Full-disc vector ma...

  11. First use of synoptic vector magnetograms for global nonlinear force free coronal magnetic field models

    CERN Document Server

    Tadesse, Tilaye; Gosain, S; MacNeice, P; Pevtsov, Alexei A

    2013-01-01

    The magnetic field permeating the solar atmosphere is generally thought to provide the energy for much of the activity seen in the solar corona, such as flares, coronal mass ejections (CMEs), etc. To overcome the unavailability of coronal magnetic field measurements, photospheric magnetic field vector data can be used to reconstruct the coronal field. Currently there are several modelling techniques being used to calculate three-dimension of the field lines into the solar atmosphere. For the first time, synoptic maps of photospheric vector magnetic field synthesized from Vector Spectromagnetograph (VSM) on Synoptic Optical Long-term Investigations of the Sun (SOLIS) are used to model the coronal magnetic field and estimate free magnetic energy in the global scale. The free energy (i.e., the energy in excess of the potential field energy) is one of the main indicators used in space weather forecasts to predict the eruptivity of active regions. We solve the nonlinear force-free field equations using optimizatio...

  12. Natural populations of lactic acid bacteria isolated from vegetable residues and silage fermentation.

    Science.gov (United States)

    Yang, J; Cao, Y; Cai, Y; Terada, F

    2010-07-01

    Natural populations of lactic acid bacteria (LAB) and silage fermentation of vegetable residues were studied. Fifty-two strains of LAB isolated from cabbage, Chinese cabbage, and lettuce residues were identified and characterized. The LAB strains were gram-positive and catalase-negative bacteria, which were divided into 6 groups (A to F) according to morphological and biochemical characteristics. The strains in group A were rods that did not produce gas from glucose and formed the d and l isomers of lactate. Groups B and C were homofermentative cocci that formed l-lactic acid. Groups D, E, and F were heterofermentative cocci that formed d-lactic acid. Based on 16S rDNA gene sequence analysis, group A to F strains were identified as Lactobacillus plantarum, Lactococcus piscium, Lactococcus lactis, Leuconostoc citreum, Weissella soli and Leuconostoc gelidum, respectively. The prevalent LAB, predominantly homofermentative lactobacilli, consisted of Lactobacillus plantarum (34.6%), Weissella soli (19.2%), Leuconostoc gelidum (15.4%), Leuconostoc citreum (13.5%), Lactococcus lactis (9.6%), and Lactococcus piscium (7.7%). Lactobacillus plantarum was the dominant member of the LAB population in 3 types of vegetable residues. These vegetable residues contained a high level of crude protein (20.2 to 28.4% of dry matter). These silages prepared by using a small-scale fermentation system were well preserved, with low pH and a relatively high content of lactate. This study suggests that the vegetable residues contain abundant LAB species and nutrients, and that they could be well preserved by making silage, which is a potentially good vegetable protein source for livestock diets.

  13. Roseomonas oryzicola sp. nov., isolated from the rhizosphere of rice (Oryza sativa L.).

    Science.gov (United States)

    Chung, Eu Jin; Yoon, Hwan Sik; Kim, Kyung Hyun; Jeon, Che Ok; Chung, Young Ryun

    2015-12-01

    A Gram-stain-negative, coccobacilli-shaped bacterium, designated YC6724T, was isolated from the rhizosphere of rice in Jinju, Korea. The taxonomy of strain YC6724T was studied using a polyphasic approach. Strain YC6724T grew optimally at 30 °C and pH 7.0-8.0. Comparative 16S rRNA gene sequence analyses showed that the strain was most closely related to Roseomonas soli 5N26T (98.4 % 16S rRNA gene sequence similarity), Roseomonas lacus THG33T(97.3 %) and Roseomonas terrae DS-48T (97.3 %). Sequence similarities with other species of the genus Roseomonas with validly published names were lower than 94.0 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain YC6724T formed a distinct phyletic lineage within the genus Roseomonas. Strain YC6724T had DNA-DNA relatedness values of 16.6 %, 44.0 % and 33.2 % with R. soli KACC 16376T, R. terrae KACC 12677T and R. lacus KACC 11678T, respectively. The predominant fatty acids of strain YC6724T were C18 : 1ω7c and/or C18 : 1ω6c, C16 : 0 and C18 : 1 2-OH. The polar lipid profile contained phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol,diphosphatidylglycerol, an unknown aminolipid and two unknown lipids. The G+C content of the genomic DNA was 70.5 mol% and the major quinone was Q-10. Strain YC6724T contained spermidine as the major polyamine. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain YC6724T represents a novel species of the genus Roseomonas, for which the name Roseomonas oryzicola sp. nov. is proposed. The type strain is YC6724T (=KCTC 22478T=NBRC 109439T).

  14. Roseomonas eburnea sp. nov., isolated from activated sludge.

    Science.gov (United States)

    Wang, Chenghong; Deng, Shikai; Liu, Xin; Yao, Li; Shi, Chao; Jiang, Jin; Kwon, Soon-Wo; He, Jian; Li, Jiayou

    2016-01-01

    A Gram-stain-negative, aerobic, short rod-shaped, non-endospore-forming, ivory-pigmented and non-motile bacterium, designated strain BUT-5T, was isolated from activated sludge of an herbicides-manufacturing wastewater treatment facility in Jiangsu Province, China. The major fatty acids (>5 % of total fatty acids) were C16 : 0, C18 : 1 2-OH and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The predominant respiratory quinone was ubiquinone Q-10. The polar lipids profile of strain BUT-5T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unknown aminolipids. The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BUT-5T showed the highest sequence similarities to Roseomonas soli 5N26T (97.5 % 16S rRNA gene sequence similarity), followed by Roseomonas lacus TH-G33T (97.3 %) and Roseomonas terrae DS-48T (97.1 %). Strain BUT-5T showed low DNA-DNA relatedness with Roseomonas soli KACC 16376T (41 %), Roseomonas lacus KACC 11678T (46 %) and Roseomonas terrae KACC 12677T (42 %), respectively. On the basis of phenotypic and genotypic properties, as well as chemotaxonomic data, strain BUT-5T represents a novel species of the genus Roseomonas, for which the name Roseomonas eburnea sp. nov. is proposed. The type strain is BUT-5T ( = CCTCC AB2013276T = KACC 17166T).

  15. Magnetic Helicity of the Global Field in Solar Cycles 23 and 24

    Science.gov (United States)

    Pipin, V. V.; Pevtsov, A. A.

    2014-07-01

    For the first time we reconstruct the magnetic helicity density of the global axisymmetric field of the Sun using the method proposed by Brandenburg et al. and Pipin et al. To determine the components of the vector potential, we apply a gauge which is typically employed in mean-field dynamo models. This allows for a direct comparison of the reconstructed helicity with the predictions from the mean-field dynamo models. We apply this method to two different data sets: the synoptic maps of the line-of-sight magnetic field from the Michelson Doppler Imager (MDI) on board the Solar and Heliospheric Observatory (SOHO) and vector magnetic field measurements from the Vector Spectromagnetograph (VSM) on the Synoptic Optical Long-term Investigations of the Sun (SOLIS) system. Based on the analysis of the MDI/SOHO data, we find that in solar cycle 23 the global magnetic field had positive (negative) magnetic helicity in the northern (southern) hemisphere. This hemispheric sign asymmetry is opposite to the helicity of the solar active regions, but it is in agreement with the predictions of mean-field dynamo models. The data also suggest that the hemispheric helicity rule may have reversed its sign during the early and late phases of cycle 23. Furthermore, the data indicate an imbalance in magnetic helicity between the northern and southern hemispheres. This imbalance seems to correlate with the total level of activity in each hemisphere in cycle 23. The magnetic helicity for the rising phase of cycle 24 is derived from SOLIS/VSM data, and qualitatively its latitudinal pattern is similar to the pattern derived from SOHO/MDI data for cycle 23.

  16. Microlunatus cavernae sp. nov., a novel actinobacterium isolated from Alu ancient cave, Yunnan, South-West China.

    Science.gov (United States)

    Cheng, Juan; Chen, Wei; Huo-Zhang, Bing; Nimaichand, Salam; Zhou, En-Min; Lu, Xin-Hua; Klenk, Hans-Peter; Li, Wen-Jun

    2013-07-01

    A Gram-positive, coccoid, non-endospore-forming actinobacterium, designated YIM C01117(T), was isolated from a soil sample collected from Alu ancient cave, Yunnan province, south-west China. Based on the 16S rRNA gene sequence analysis, strain YIM C01117(T) was shown to belong to the genus Microlunatus, with highest sequence similarity of 97.4 % to Microlunatus soli DSM 21800(T). The whole genomic DNA relatedness as shown by the DNA-DNA hybridization study between YIM C01117(T) and M. soli DSM 21800(T) had a low value (47 ± 2 %). Strain YIM C01117(T) was determined to contain LL-diaminopimelic acid with Gly, Glu and Ala amino acids (A3γ' type) in the cell wall. Whole-cell hydrolysates were found to contain glucose, galactose, mannose and ribose. The major polar lipids were determined to be phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinone system present is MK-9(H4), while the major fatty acids were identified to be anteiso-C15:0 (24.1 %), iso-C16:0 (22.3 %) and iso-C15:0 (11.4 %). The G+C content of the genomic DNA was determined to be 65.9 mol%. The chemotaxonomic and genotypic data support the affiliation of the strain YIM C01117(T) to the genus Microlunatus. The results of physiological and biochemical tests allow strain YIM C01117(T) to be differentiated phenotypically from recognized Microlunatus species. Strain YIM C01117(T) is therefore considered to represent a novel species of the genus Microlunatus, for which the name Microlunatus cavernae sp. nov. is proposed. The type strain is YIM C01117(T) (= DSM 26248(T) = JCM 18536(T)).

  17. Lietuvių kalbos postpoziciniai vietininkai

    Directory of Open Access Journals (Sweden)

    Zigmas Zinkevičius

    2011-12-01

    Full Text Available POSTPOSITIONAL LOCATIVES IN LITHUANIANSummaryThe paper argues that postpositional locatives in Lithuanian (inessive, illative, adessive, allative are not formations of the same epoch, but rather they were formed at different times; the pro­cess of their formation must have been long and continued right up to the appearance of the first Lithuanian printed book when the case-forms fell into a decline. First came singular forms, next — plural forms. More complicated and protracted was the development of inessive and adessive case-forms.Ā- and ē-stem sg. iness. and ill. forms (e. g. šakojè, žẽmėje, šakõn, žẽmėn are considered to be the oldest. The other sg. ill. and particularly sg. iness. forms are products of later development, as they are, as a rule, constructed in the above-mentioned pattern. The paper gives a detailed analysis of the case-forms and on the basis of the analysis it argues that the singular of all postpositional locatives had already been formed and become stable by the beginning of the period of writing -nothing of the kind can be said of the plural. Only illative and allative possessed then stable plural forms.The development of pl. iness. and adess. forms was hindered by the residual ancient Indo-European locative in -su. The centre of the author's attention, however, is the interrelationship of the case-forms and particularly the origin and development of pl. iness. and adess. case-forms.On the basis of a detailed analysis of the old writings, the author argues that the pl. iness. šakosa derives not from šakose, as is generally assumed, but it presents by itself a transformed version of the older form šakosu. The form šakose is to be derived not from the pl. acc. * šakā́s + *éп, but it should be treated as a transform of šakosu от šakosa in -e from the sg. inessive, cf. šakojè. Pl. adess. šakosumpi is not a transform of šakosempi, as is generally assumed, but the older variant of the

  18. Inter- and intra-annual dynamics of height increment in young beech and spruce stands in relation to tree size and weather conditions

    Directory of Open Access Journals (Sweden)

    Konôpka Bohdan

    2014-03-01

    Full Text Available Počas rokov 2009-2013 sme sledovali sezónnu dynamiku výškového prírastku, ako aj celoročný výškový prírastok v bukových a smrekových mladinách rastúcich na totožnom stanovišti. Výsledky naznačili, že medziročné zmeny základných porastových charakteristík, a to najmä hustota porastu, boli výraznejšie v smrečine než v bučine. Omnoho vyššia stromová mortalita v smrečine sa vysvetlila nižšou intenzitou svetla pod korunovou vrstvou smrekov, resp. vyššou toleranciou buka na zatienenie. Veľké medzidruhové rozdiely sa zaznamenali v „načasovaní“ výškového prírastku. Konkrétne výškový prírastok bukov začal každoročne skôr ako výškový prírastok smrekov. Ďalej, terminál smrekov rástol dlhšie (približne 70 dní v porovnaní s bukmi (45 dní. Porovnanie priebehov výškových prírastkov vzhľadom na prostredie naznačilo, že principiálny vplyv mala globálna radiácia. V bučine bola maximálna intenzita výškového prírastku počas dní s maximálnou úrovňou globálnej radiácia, a to v rokoch 2009 a 2011, zároveň pokles intenzity sa objavil simultánne s poklesom globálnej radiácie počas roku 2012. V smrečine intenzita výškového prírastku predstavovala jednovrcholovú krivku - s najvyššou intenzitou prírastku v dňoch maximálnych hodnôt globálnej radiácie počas 2009 a 2012. Na druhej strane sa zaznamenal dvojvrcholový výskyt maxím intenzity výškového prírastku smrekov v 2010 a 2011. Tento jav súvisel s dočasným poklesom globálnej radiácie v týchto rokoch. Medzidruhové rozdiely v načasovaní výškového prírastku môžu mať relevanciu v prípade výskytu nepriaznivých poveternostných podmienok. Napríklad pri epizódach sucha v polovici vegetačného obdobia, keďže výškový prírastok je vtedy už ukončený pri bukoch avšak na smrekoch ešte prebieha

  19. Selected Issues of Legislation with regard to Operating Pharmacies in the Slovak Republic / Vybrané problémy právnej úpravy prevádzkovania lekární v podmienkach Slovenskej republiky

    Directory of Open Access Journals (Sweden)

    Capandová Petra

    2015-06-01

    Full Text Available Medzi základné práva a slobody, upravené v druhej hlave Ústavy Slovenskej republiky patrí aj právo na ochranu zdravia, bezplatnú zdravotnú starostlivosť na základe zdravotného poistenia, právo na zdravotnícke pomôcky za zákonom stanovených podmienok. Tieto práva sú garantované v ustanovení Čl. 40 Ústavy Slovenskej republiky v rámci ekonomických, sociálnych a kultúrnych práv a vzťahujú sa na každú fyzickú osobu, t. j. na človeka. Keďže sú tieto práva považované za ľudské práva v zmysle medzinárodného práva, sú garantované aj v mnohostranných medzinárodných zmluvách, ktorých signatárom je aj Slovenská republika, ako napríklad Medzinárodný pakt o hospodárskych, sociálnych a kultúrnych právach. Právna úprava prevádzkovania lekární v Slovenskej republike zahŕňa široké spektrum otázok a je pomerne rozsiahla. Snahou zákonodarcu bolo nastaviť také pravidlá, ktoré by stransparentnili a zjednodušili právne prostredie v tomto sektore. V niektorých oblastiach sú pravidlá dané legislatívou Európskej únie a slovenský právny poriadok ich iba prevzal. Najdôležitejším prvokom v tejto sfére, ktorý nie je priamo upravovaný legislatívou EÚ, je poskytovanie lekárenskej starostlivosti a využívanie systému verejného zdravotného poistenia v tejto oblasti. Hlavným cieľom príspevku je poukázať na vybrané problémy, ktoré sa spájajú s postavením prevádzkovateľa lekárne a jeho povinnosťami, ktoré mu vyplývajú zo zákona o liekoch a s ktorými sa musí ako prevádzkovateľ lekárne vysporiadať. Táto oblasť podnikania je pomerne prísne regulovaná a preto je potrebné byť oboznámený s príslušnými právnymi predpismi. Príspevok z právno-teoretického hľadiska rieši viaceré otázky upravené v zákone o liekoch, či vyhláške o správnej lekárenskej praxi.

  20. In vivo analysis of MEF2 transcription factors in synapse regulation and neuronal survival.

    Directory of Open Access Journals (Sweden)

    M Waseem Akhtar

    Full Text Available MEF2 (A-D transcription factors govern development, differentiation and maintenance of various cell types including neurons. The role of MEF2 isoforms in the brain has been studied using in vitro manipulations with only MEF2C examined in vivo. In order to understand specific as well as redundant roles of the MEF2 isoforms, we generated brain-specific deletion of MEF2A and found that Mef2aKO mice show normal behavior in a range of paradigms including learning and memory. We next generated Mef2a and Mef2d brain-specific double KO (Mef2a/dDKO mice and observed deficits in motor coordination and enhanced hippocampal short-term synaptic plasticity, however there were no alterations in learning and memory, Schaffer collateral pathway long-term potentiation, or the number of dendritic spines. Since previous work has established a critical role for MEF2C in hippocampal plasticity, we generated a Mef2a, Mef2c and Mef2d brain-specific triple KO (Mef2a/c/dTKO. Mef2a/c/d TKO mice have early postnatal lethality with increased neuronal apoptosis, indicative of a redundant role for the MEF2 factors in neuronal survival. We examined synaptic plasticity in the intact neurons in the Mef2a/c/d TKO mice and found significant impairments in short-term synaptic plasticity suggesting that MEF2C is the major isoform involved in hippocampal synaptic function. Collectively, these data highlight the key in vivo role of MEF2C isoform in the brain and suggest that MEF2A and MEF2D have only subtle roles in regulating hippocampal synaptic function.

  1. In Vivo Analysis of MEF2 Transcription Factors in Synapse Regulation and Neuronal Survival

    Science.gov (United States)

    Adachi, Megumi; Morris, Michael J.; Qi, Xiaoxia; Richardson, James A.; Bassel-Duby, Rhonda; Olson, Eric N.; Kavalali, Ege T.; Monteggia, Lisa M.

    2012-01-01

    MEF2 (A–D) transcription factors govern development, differentiation and maintenance of various cell types including neurons. The role of MEF2 isoforms in the brain has been studied using in vitro manipulations with only MEF2C examined in vivo. In order to understand specific as well as redundant roles of the MEF2 isoforms, we generated brain-specific deletion of MEF2A and found that Mef2aKO mice show normal behavior in a range of paradigms including learning and memory. We next generated Mef2a and Mef2d brain-specific double KO (Mef2a/dDKO) mice and observed deficits in motor coordination and enhanced hippocampal short-term synaptic plasticity, however there were no alterations in learning and memory, Schaffer collateral pathway long-term potentiation, or the number of dendritic spines. Since previous work has established a critical role for MEF2C in hippocampal plasticity, we generated a Mef2a, Mef2c and Mef2d brain-specific triple KO (Mef2a/c/dTKO). Mef2a/c/d TKO mice have early postnatal lethality with increased neuronal apoptosis, indicative of a redundant role for the MEF2 factors in neuronal survival. We examined synaptic plasticity in the intact neurons in the Mef2a/c/d TKO mice and found significant impairments in short-term synaptic plasticity suggesting that MEF2C is the major isoform involved in hippocampal synaptic function. Collectively, these data highlight the key in vivo role of MEF2C isoform in the brain and suggest that MEF2A and MEF2D have only subtle roles in regulating hippocampal synaptic function. PMID:22496871

  2. Enhancement of Adipocyte Browning by Angiotensin II Type 1 Receptor Blockade

    Science.gov (United States)

    Tsukuda, Kana; Mogi, Masaki; Iwanami, Jun; Kanno, Harumi; Nakaoka, Hirotomo; Wang, Xiao-Li; Bai, Hui-Yu; Shan, Bao-Shuai; Kukida, Masayoshi; Higaki, Akinori; Yamauchi, Toshifumi; Min, Li-Juan; Horiuchi, Masatsugu

    2016-01-01

    Browning of white adipose tissue (WAT) has been highlighted as a new possible therapeutic target for obesity, diabetes and lipid metabolic disorders, because WAT browning could increase energy expenditure and reduce adiposity. The new clusters of adipocytes that emerge with WAT browning have been named ‘beige’ or ‘brite’ adipocytes. Recent reports have indicated that the renin-angiotensin system (RAS) plays a role in various aspects of adipose tissue physiology and dysfunction. The biological effects of angiotensin II, a major component of RAS, are mediated by two receptor subtypes, angiotensin II type 1 receptor (AT1R) and type 2 receptor (AT2R). However, the functional roles of angiotensin II receptor subtypes in WAT browning have not been defined. Therefore, we examined whether deletion of angiotensin II receptor subtypes (AT1aR and AT2R) may affect white-to-beige fat conversion in vivo. AT1a receptor knockout (AT1aKO) mice exhibited increased appearance of multilocular lipid droplets and upregulation of thermogenic gene expression in inguinal white adipose tissue (iWAT) compared to wild-type (WT) mice. AT2 receptor-deleted mice did not show miniaturization of lipid droplets or alteration of thermogenic gene expression levels in iWAT. An in vitro experiment using adipose tissue-derived stem cells showed that deletion of the AT1a receptor resulted in suppression of adipocyte differentiation, with reduction in expression of thermogenic genes. These results indicate that deletion of the AT1a receptor might have some effects on the process of browning of WAT and that blockade of the AT1 receptor could be a therapeutic target for the treatment of metabolic disorders. PMID:27992452

  3. Dnmt3a Regulates Proliferation of Muscle Satellite Cells via p57Kip2

    Science.gov (United States)

    Naito, Masashi; Mori, Masaki; Inagawa, Masayo; Miyata, Kohei; Hashimoto, Naohiro; Tanaka, Sakae; Asahara, Hiroshi

    2016-01-01

    Cell differentiation status is defined by the gene expression profile, which is coordinately controlled by epigenetic mechanisms. Cell type-specific DNA methylation patterns are established by chromatin modifiers including de novo DNA methyltransferases, such as Dnmt3a and Dnmt3b. Since the discovery of the myogenic master gene MyoD, myogenic differentiation has been utilized as a model system to study tissue differentiation. Although knowledge about myogenic gene networks is accumulating, there is only a limited understanding of how DNA methylation controls the myogenic gene program. With an aim to elucidate the role of DNA methylation in muscle development and regeneration, we investigate the consequences of mutating Dnmt3a in muscle precursor cells in mice. Pax3 promoter-driven Dnmt3a-conditional knockout (cKO) mice exhibit decreased organ mass in the skeletal muscles, and attenuated regeneration after cardiotoxin-induced muscle injury. In addition, Dnmt3a-null satellite cells (SCs) exhibit a striking loss of proliferation in culture. Transcriptome analysis reveals dysregulated expression of p57Kip2, a member of the Cip/Kip family of cyclin-dependent kinase inhibitors (CDKIs), in the Dnmt3a-KO SCs. Moreover, RNAi-mediated depletion of p57Kip2 replenishes the proliferation activity of the SCs, thus establishing a role for the Dnmt3a-p57Kip2 axis in the regulation of SC proliferation. Consistent with these findings, Dnmt3a-cKO muscles exhibit fewer Pax7+ SCs, which show increased expression of p57Kip2 protein. Thus, Dnmt3a is found to maintain muscle homeostasis by epigenetically regulating the proliferation of SCs through p57Kip2. PMID:27415617

  4. Vplyv sofistikovaného hybridného Honeypotu na efektivitu architektúry systému detekcie prieniku v distribuovaných počítačových

    Directory of Open Access Journals (Sweden)

    Martin Chovanec

    2013-06-01

    Full Text Available Pri súčasnom vývoji technológií, rapídnom raste počítačových sietí a distribuovaných systémov, je reálne riziko útoku čoraz pravdepodobnejšie. Pre zvýšenie samotnej bezpečnosti systémov už bolo vytvorených a implementovaných množstvo riešení, ktoré mali slúžiť na detekciu a/alebo prevenciu pred samotnými útokmi. Najpoužívanejšie riešenie predstavuje použitie systému na detekciu prieniku (IDS v kooperácii s firewallom. Avšak ani IDS a ani firewall nedokážu reagovať v reálnom čase, pokiaľ sa jedná o špecifický typ útoku. Táto práca sa zaoberá detekčným mechanizmom na báze technológie Honeypot a jeho využitím v navrhovanej architektúre pre zvýšenie bezpečnosti v počítačových systémoch. Podstatou práce je poukázať na to, ako dokáže sofistikovaný hybridný Honeypot vplývať na dizajn architektúry IDS a tým zvýšiť jej efektivitu.

  5. Ugovor o zajedničkom pothvatu (joint venture u naftnom sektoru

    Directory of Open Access Journals (Sweden)

    Ratko Brnabić

    2014-10-01

    Full Text Available U radu se raspravlja o ugovoru o zajedničkom pothvatu koji je praktički neizostavan dio svake ozbiljnije operacije istraživanja, a potom i iskorištavanja ugljikovodika (proizvodnja naftnih derivata. Riječ je o skupom pothvatu uz koji se vezuju brojni rizici pa trgovci u tom području žele sklapanjem takvog ugovora zadržati mogućnost (izglede da ostvare dobit, ali pritom podijele rizik. U prvom dijelu rada daje se prikaz bitnih značajki zajedničkog pothvata s posebnim osvrtom na područje iskorištavanja ugljikovodika. S obzirom na okolnost da se za potrebe zajedničkog pothvata često osniva trgovačko društvo kapitala, raspravlja se o pravnim odnosima članova tog društva međusobno te prema upravi društva. Skreće se pozornost i na razlike u značenju pojma joint venture posebice u američkom i hrvatskom pravu što može izazvati ozbiljne pravne učinke ako ortaci nemaju sjedište u istoj državi. Nakon toga, obrađuju se bitna pitanja koja ugovorne strane neizostavno moraju urediti da bi se izbjegli sudski sporovi. To su, primjerice, pitanje područja istraživanja, pravni položaj operatora, sredstva osiguranja, standard kvalitete pri obavljanju poslova, pravo na informiranje i nadzor te pitanje sustava rješavanja sukoba u ortaštvu. U zaključku se iznose rezultati istraživanja te se upućuje na moguća rješenja za otvorena pitanja uređenja međusobnog odnosa ortaka u zajedničkom pothvatu.

  6. Dnmt3a Regulates Proliferation of Muscle Satellite Cells via p57Kip2.

    Directory of Open Access Journals (Sweden)

    Masashi Naito

    2016-07-01

    Full Text Available Cell differentiation status is defined by the gene expression profile, which is coordinately controlled by epigenetic mechanisms. Cell type-specific DNA methylation patterns are established by chromatin modifiers including de novo DNA methyltransferases, such as Dnmt3a and Dnmt3b. Since the discovery of the myogenic master gene MyoD, myogenic differentiation has been utilized as a model system to study tissue differentiation. Although knowledge about myogenic gene networks is accumulating, there is only a limited understanding of how DNA methylation controls the myogenic gene program. With an aim to elucidate the role of DNA methylation in muscle development and regeneration, we investigate the consequences of mutating Dnmt3a in muscle precursor cells in mice. Pax3 promoter-driven Dnmt3a-conditional knockout (cKO mice exhibit decreased organ mass in the skeletal muscles, and attenuated regeneration after cardiotoxin-induced muscle injury. In addition, Dnmt3a-null satellite cells (SCs exhibit a striking loss of proliferation in culture. Transcriptome analysis reveals dysregulated expression of p57Kip2, a member of the Cip/Kip family of cyclin-dependent kinase inhibitors (CDKIs, in the Dnmt3a-KO SCs. Moreover, RNAi-mediated depletion of p57Kip2 replenishes the proliferation activity of the SCs, thus establishing a role for the Dnmt3a-p57Kip2 axis in the regulation of SC proliferation. Consistent with these findings, Dnmt3a-cKO muscles exhibit fewer Pax7+ SCs, which show increased expression of p57Kip2 protein. Thus, Dnmt3a is found to maintain muscle homeostasis by epigenetically regulating the proliferation of SCs through p57Kip2.

  7. »Kommunikation und Kybernetik in Einzeldarstellungen”. Herausgegeben von H. Wolter und W. D. Keidel. Band 2: Structural Linguistics and Human Communication. An Introduction into the Mechanism of Language and the Methodology of Linguistics By Bertil Malmbe

    Directory of Open Access Journals (Sweden)

    Žarko Muljačić

    1966-12-01

    Full Text Available Za razliku od priručnika istog pisca New Trends in Linguistics. An Orientation, Stockholm-Lund 1964 (koji predstavlja dotjerani i ažurirani prijevod švedskog originala iz 1959. god. i o kome v. recenziju koju smo objavili u »ZFLMS« X, Novi Sad 1967, str. 181-188, ova je odlična monografija namijenjena uzem krugu čitalaca koji već imaju dovoljnih znanja iz lingvistike i fonetike ili iz društvenih i prirodnih nauka, kojima su od koristi tekovine i metode suvremene lingvistike (psihologija, fonijatrija, logoterapija, metodika učenja stranih jezika, nauka o komunikacijama i sl.. Autor, ugledni romanist i profesor fonetike na sveučilištu u Lundu, a od nedavna i na Sorbonni, svijestan je opasnosti da će lingvistika, prikazana prvenstveno kao znanost koja studira jezično komuniciranje ljudi, nužno morati da se pozabavi i vrlo širokim krugom problema pa će tako neka poglavlja ispasti za jedne čitaoce prekomplicirapa a za druge preelementarna. Drugačije međutim nije moglo biti ako se željelo na relativno malenom prostoru prikazati tako različite pojmove kao sto su fonemi i značenje, decibeli i jezicna promjena, knjizevni stil i govorne smetnje. Metodski pristup gradi i kompozicija sasvim su drugačiji nego u New Trends in Linguistics. Dok su tamo poglavlja posvečena pojedinim lingvističkim školama i pravcima (a samo indirektno i nekim skupinama problema ukoliko se neka škola uglavnom njiina bavila, ova je monografija obrađena problemski.

  8. Modified Zeolites in Ground Water Treatment/ Modifikované Zeolity V Úprave Podzemných Vôd

    Directory of Open Access Journals (Sweden)

    Barloková Danka

    2015-03-01

    Full Text Available Článok prezentuje výsledky technologických skúšok vykonaných v UV Kúty. Cieľom tejto práce bolo porovnať modifikované (povrchovo upravené zeolity známe ako klinoptilolit (veľké nálezisko klinoptilolitu bolo objavené na Východnom Slovensku v 1980-tych rokoch s dovážaným povrchovo upraveným zeolitom z náleziska v Maďarsku. Klinopur-Mn a Klinomangan boli použité pre odstraňovanie železa a mangánu z podzemnej vody na dosiahnutie limitných hodnôt pre pitnú vodu podľa Nariadenia vlády č. 496/2010 Z.z. Sledované materiály vykazovali rôznu účinnosť odstraňovania mangánu z vody, na účinnosť odstraňovania mala významný vplyv kvalita upravovanej vody (obsah kyslíka, hodnota pH. V prípade odstraňovania železa z vody kvalita surovej vody nie je limitujúcim faktorom, obidva materiály odstraňovali železo z vody pod limitnú hodnotu (0,2 mg.l-1.

  9. Chuck Palahniuk: Negdje mora puknuti / S engleskog prevela Valentina Lisak

    Directory of Open Access Journals (Sweden)

    Chuck Palahniuk

    2011-12-01

    Full Text Available Iza zaključanih vrata zahoda u stražnjem dijelu antikvarnice Claire Upton razgovara na telefon. Glas joj odjekuje od poda i zidova: Koliko je teško provaliti nadzornu kameru? Ukrasti snimku? upita svog muža i počne plakati.U zadnjih tjedan dana bila je u tom dućanu već tri ili četiri puta. Jedan od onih gdje moraš ostaviti torbu na blagajni, inače te ne puste unutra. Moraš ostaviti i kaput, ako ima duboke, prostrane džepove. I kišobran, jer bi ljudi u nabore mogli ubacivati sitne predmete, češljeve, nakit i druge drangulije. Kraj starog blagajnika stoji sivi karton na kojem crnim markerom piše: “Ne volimo kad nas kradete!”Skidajući kaput, Claire reče: “Ja nisam lopov.”Stari blagajnik pogledom ju je odmjerio od glave do pete. Coknuo je jezikom i pitao: “Po čemu ste vi iznimka?”Za svaki ostavljeni predmet dao je Claire polovicu karte. Za torbicu hercova asa. Za kaput trefovu devetku. Za kišobran pikovu trojku.Pogledom je prešao preko Claireinih ruku, obrisa džepova na prsima i najlonki tražeći izbočine, nešto ukradeno. Iza prednjeg pulta po cijeloj su trgovini visjeli mali natpisi koji su upozoravali da je ovdje zabranjeno krasti. Kamere su nadzirale svaki prolaz, svaki kutak i sve prikazivale na malenom ekranu punom drugih ekrana. Trezor malih, crno-bijelih tv-ekrana iza blagajne gdje je sjedio starac i sve ih promatrao.

  10. The Evaluation of Selected Air Pollutants in Košice for the Period 2008-2010 / Analýza Znečisťujúcich Látok Vo Vybraných Monitorovacích Staniciach Mesta Košice Za Obdobie 2008 - 2010

    Directory of Open Access Journals (Sweden)

    Gergeľová Marcela

    2014-03-01

    Full Text Available Vo všeobecnosti kvalita ovzdušia je stanovená z obsahu znečisťujúcich látok vo vonkajšom ovzduší. Kritériá kvality ovzdušia (limitné a cieľové hodnoty, medze tolerancie, horné a dolné medze na hodnotenie vychádzajú z platného legislatívneho rámca. V podmienkach SR kritéria kvality ovzdušia vyplývajú z vyhlášky MŽP SR č. 360/2010 Z.z. o kvalite ovzdušia [1]. V nadväznosti na realizované merania je potrebné zvoliť efektívne nástroje potrebné pre preprocesing a postprocesing procesu plošného hodnotenia kvality ovzdušia. Príspevok si kladie za cieľ aplikovať vhodné nástroje GIS do hodnotiaceho procesu kvality ovzdušia v aglomerácií Košice za vybrané obdobie rokov 2008 - 2010. Pri celkovom zhodnotení predmetnej oblasti bolo uvažované so spracovaním informácií o emisiách deklarovaných ako tuhé znečisťujúce látky (TZL v zmysle konzervatívneho prístupu k hodnoteniu kvality ovzdušia za PM10. Pre posudzovanie boli použité ročenky a správy pre kvalitu ovzdušia v SR za rok 2008 - 2010 z SHMÚ a odborné publikácie

  11. Informacioni sistemi održavanja u Vojsci Srbije i Crne Gore i okruženju / Informational systems of maintenance in the Army of Serbia and Montenegro and environment

    Directory of Open Access Journals (Sweden)

    Saša Veselinović

    2005-05-01

    Full Text Available Efikasno upravljanje sistemom održavanja moguće je samo ako vrh upravljačke strukture ima pravovremene i kvalitetne informacije o stanju sistema. Ovakve informacije moguće je obezbediti primenom odgovarajućeg automatizovanog informacionog sistema. U svetu postoje programski paketi za automatizaciju informacionog sistema održavanja pomoću kojih se vode podaci o stanju sredstava, toku održavanja, troškovima, nabavci rezervnih delova, a pojedini sistemi omogućavaju i statističko predviđanje otkaza. Informacioni sistem održavanja koji funkcioniše u Vojsci Srbije i Crne Gore je manuelan, a uspostavljen je pre uvođenja računara u operativnu upotrebu. Tokom eksploatacije sistem je više puta dograđivan i prilagođavan za automatizaciju. Aplikacije koje su trenutno u funkciji u pojedinim jedinicama nisu sistemsko rešenje već pokušaji entuzijasta da olakšaju deo aktivnosti u svom radu, a vezane su prvenstveno za izradu izveštaja. / Management of system of maintenance on effective -way is possible only if management has opportune and quality information about state of system. Providing opportune and quality information is possible only with appropriate automotive information system. In The World exist many programs for automation information systems of maintenance, -which observe a data about condition of equipments, flow of maintenance, a costs, purchasing of pans, and some of them provide statistical predicting of failures. Information system of maintenance, which functioning in Military forces of State Union of Serbia and Montenegro, is manual and established before implementation of personal computer in operative use, but through couple projects accomplished for automation. A program, which are in use, in some units, aren't system solution, then attempting group enthusiastic people to make easiest some activity in his work on making reports.

  12. Predictability of GNSS signal observations in support of Space Situational Awareness using passive radar

    Science.gov (United States)

    Mahmud, M. S.; Lambert, A.; Benson, C.

    2015-07-01

    GNSS signals have been proposed as emitters of opportunity to enhance Space Situational Awareness (SSA) by tracking small items of space debris using bistatic radar. Although the scattered GNSS signal levels from small items of space debris are incredibly low, the dynamic disturbances of the observed object are very small, and the phase of the scattered signals is well behaved. It is therefore plausible that coherent integration periods on the order of many minutes could be achieved. However, even with long integration periods, very large receiver arrays with extensive, but probably viable, processing are required to recover the scattered signal. Such large arrays will be expensive, and smaller more affordable arrays will collect insufficient signal power to detect the small objects (relative to wavelength) that are necessary to maintain the necessary phase coherency. The investments necessary to build a large receiver array are unlikely without substantial risk reduction. Pini and Akos have previously reported on use of very large radio telescopes to analyse the short-term modulation performance of GNSS satellite signals. In this work we report on tracking of GPS satellites with a radio-astronomy VLBI antenna system to assess the stability of the observed GPS signal over a time period indicative of that proposed for passive radar. We also confirm some of the processing techniques that may be used in both demonstrations and the final system. We conclude from the limited data set that the signal stability when observed by a high-gain tracking antenna and compared against a high quality, low phase-noise clock is excellent, as expected. We conclude by framing further works to reduce risk for a passive radar SSA capability using GNSS signals. http://www.ignss.org/Conferences/PastConferencePapers/2015ConferencePastPapers/2015PeerReviewedPapers/tabid/147/Default.aspx

  13. REFORMA EKONOMIJE

    Directory of Open Access Journals (Sweden)

    Joseph Stiglitz

    2013-12-01

    Full Text Available Dosta je krivice koja se deli u ovoj krizi. Videli smo ulogu regulatornih tela i zakonodavaca, FED-a i finansijera, svih njih koji su bili u igri. Svako od njih je govorio o svom interesu zauzimajući stav da su bili u pravu za ono što su radili a veoma često zasnivajući svoje stavove na ekonomskoj analizi. Kada počne raslojavanje pitanja šta je pošlo naopako ne može se izostaviti fokus sa ekonomske profesije. Naravno, nisu se svi ekonomisti pridružili slavi slobodnog ekonomskog tržišta i nisu svi bili sledbenici Miltona Fridmana. Iznenađujuće je što je ipak, veliki deo bio naklonjen tom pravcu. Ne samo da je njihov savet bio pogrešan već su bile pogrešne i osnovne pretpostavke i predviđanja. Relativno mali broj njih je mogao da vidi nadolazeću katastrofu. Nije bilo slučajno da su oni, koji su se zalagali za pravila koja su dovela do nesreće, bili toliko zaslepljeni svojom verom u slobodno tržište te nisu mogli da vide probleme koji nastaju. Ekonomija se promenila više nego što su to ekonomisti želeli da veruju, do te mere da je od naučne discipline postala navijačica discipline slobodnog tržišta. Ako SAD želi da uspe u reformi privrede, moraće da počne sa reformom ekonomije.

  14. Above-ground net primary productivity in young stands of beech and spruce

    Directory of Open Access Journals (Sweden)

    Pajtík Jozef

    2013-09-01

    Full Text Available V tejto práci sme pomocou kombinácií kontinuálnych meraní a deštruktívnych odberov vzorníkov sledovali a porovnali zásobu nadzemnej biomasy a ročnú primárnu produkciu (NPP v prirodzene obnovených mladých porastoch buka a smreka. Na vybranej lokalite predpokladáme, že zmenené klimatické podmienky budú lepšie vyhovovať buku pred v súčasnosti prevládajúcim smrekom. Lokalita Vrchslatina sa nachádza v južnej časti Veporských vrchov v nadmorskej výške 977 m nad morom (48° 38΄ 50΄΄ N, 19° 36΄ 07΄΄ E. Priemerné ročné zrážky sa pohybujú okolo 900 mm, priemerná ročná teplota je 5,2 °C. Na sledovanej lokalite sme pozorovali odlišný priebeh rastu buka a smreka. Pri buku bola pozorovaná menšia medziročná mortalita, udržiaval sa až prehustený zápoj, v ktorom sa aj podúrovňové stromy snažili dostať do úrovne. Toto sa prejavilo na tvare kmeňov, ktoré sú tenké a vysoké. Štíhlostný koeficient sa pri stromoch so strednou hrúbkou a strednou výškou postupne zvyšoval od 1,19 do 1,40. Pri smreku dochádza k vyššej mortalite, vrastavé a podúrovňové stromy odumierajú z dôvodu nedostatku svetla. Stromy rastú viac do hrúbky, čo sa odráža aj na štíhlostnom koeficiente, ktorý bol po celé obdobie viac-menej konštantný a pohyboval sa v rozpätí 0,89 až 0,93 (tab. 1. Zásoby kmeňa sú pri smreku v jednotlivých rokoch o 3 - 10 m3.ha-1 väčšie ako pri buku (tab. 3. Po prepočítaní na sušinu je vplyvom rozdielnej objemovej hmotnosti (obr. 5 celková zásoba sušiny drevných častí (kmeň a konáre väčšia pri buku (tab. 3. Najväčší rozdiel medzi drevinami je v zásobe asimilačných orgánov, ktorá je pri smreku viac než trojnásobná (tab. 3 a 4. Počas rastu dochádza pri obidvoch drevinách k zvyšovaniu podielu kmeňa a znižovaniu podielu asimilačných orgánov (obr. 2. Hlavný medzidruhový rozdiel pri pomerne vyrovnaných hektárových zásobách je v

  15. Advances of DNA Sequencing Technology and Its Applications%DNA测序技术及其应用研究进展

    Institute of Scientific and Technical Information of China (English)

    刘朋虎; 林冬梅; 林占熺; 李晶

    2012-01-01

    In this paper, we introduced principles and characteristics of the first, second and third generation of sequencing technology, then the applications of second sequencing technology were described. Because of complicated operation and high cost, the first generation DNA sequencing technology represented by Sanger sequencing method can not meet the needs of large - scale sequencing. The second generation DNA sequencing technology characterized by high-throughout and low cost including Solexa sequencing technology of Illumina, and Applied Biosystems SOLiD and Roche 454 now has been used in many fields of life science research. The third-generation sequencing technology which can sequence single DNA molecular has also been arisen, but not been widely used in life science research Key words .%本文首先介绍了第一代、第二代、第三代DNA测序技术的原理、特点,在此基础上介绍了第二代测序技术在基因组测序、重测序,RNA测序,宏基因组,DNA甲基化等方面的应用.第一代测序技术以Sanger测序法为代表,操作繁琐、成本较高,不能满足大规模测序的需要.第二代测序技术以高通量、低成本为主要特点,主要包括Illumina公司的Solexa测序技术、罗氏公司的454测序技术和ABI公司的SOLiD测序技术,目前已广泛应用于生命科学研究的各个领域.第三代测序技术以单分子测序为主要特点,目前已经初见端倪,但是还没有被大规模广泛应用.

  16. In vivo translocation and toxicity of multi-walled carbon nanotubes are regulated by microRNAs

    Science.gov (United States)

    Zhao, Yunli; Wu, Qiuli; Li, Yiping; Nouara, Abdelli; Jia, Ruhan; Wang, Dayong

    2014-03-01

    We employed an in vivo Caenorhabditis elegans assay system to perform SOLiD sequencing analysis to identify the possible microRNA (miRNA) targets of multi-walled carbon nanotubes (MWCNTs). Bioinformatics analysis on targeted genes for the identified dysregulated miRNAs in MWCNT exposed nematodes demonstrates their involvement in many aspects of biological processes. We used loss-of-function mutants for the identified dysregulated miRNAs to perform toxicity assessment by evaluating functions of primary and secondary targeted organs, and found the miRNA mutants with susceptible or resistant property towards MWCNT toxicity. Both the physiological state of the intestine and defecation behavior were involved in the control of the susceptible or resistant property occurrence for specific miRNA mutants towards MWCNT toxicity. This work provides the molecular basis at the miRNA level for future chemical design to reduce the nanotoxicity of MWCNTs and further elucidation of the related toxicological mechanism.We employed an in vivo Caenorhabditis elegans assay system to perform SOLiD sequencing analysis to identify the possible microRNA (miRNA) targets of multi-walled carbon nanotubes (MWCNTs). Bioinformatics analysis on targeted genes for the identified dysregulated miRNAs in MWCNT exposed nematodes demonstrates their involvement in many aspects of biological processes. We used loss-of-function mutants for the identified dysregulated miRNAs to perform toxicity assessment by evaluating functions of primary and secondary targeted organs, and found the miRNA mutants with susceptible or resistant property towards MWCNT toxicity. Both the physiological state of the intestine and defecation behavior were involved in the control of the susceptible or resistant property occurrence for specific miRNA mutants towards MWCNT toxicity. This work provides the molecular basis at the miRNA level for future chemical design to reduce the nanotoxicity of MWCNTs and further elucidation of the

  17. Two new species of philometrid nematodes (Nematoda: Philometridae) in Epinephelus coioides (Hamilton, 1822) from the South Bali Sea, Indonesia.

    Science.gov (United States)

    Dewi, Kartika; Palm, Harry W

    2013-01-25

    Based on light and scanning electron microscopy, two new species of philometrid nematodes, Spirophilometra endangae sp. nov. and Philometra epinepheli sp. nov. (Nematoda: Dracunculoidea: Philometridae) are described from Epinephelus coioides (Hamilton, 1822) (Perciformes: Serranidae) from the South Bali Sea, Indonesia. Spirophilometra endangae sp. nov. was isolated from the fins of E. coioides. The new species can be distinguished from the most closely related S. eichleri Parukhin, 1971 by a larger total body length and the site of infection in the host. The new species differs from S. centropomi (Caballero, 1974) also in the larger body size of the gravid females and the site of infection in the host. S. en-dangae sp. nov. differs from S. pacifica (Moravec, Santana-Pineros, Gonzales-Solis & Torres-Huerta, 2007) in the struc-ture and arrangement of the spines on the middle part of the body, the infection site of the worm, the type host and the zoogeographical host distribution. Philometra epinepheli sp. nov. differs from all other Philometra spp. congeners so far recorded from Ephinepelus groupers in the total body length and the site of infection. This is the first opercula-infecting species of Philometra described from the fish family Serranidae.

  18. What can co-ethnic immigrants tell us about ethnic visions of the national self? A comparative analysis of Germany and Greece

    Directory of Open Access Journals (Sweden)

    Christin Heß

    2011-01-01

    Full Text Available Nations with a predominantly ethno-cultural self-perception and citizenship based on jus sanguinis are under pressure today to adopt more civic-territorial ideas of nationhood, including elements of jus soli. Two nations experiencing these trends in Europe but have rarely been juxtaposed are Greece and Germany. Characteristic of both nations is a long reserved privileged access to citizenship and settlement assistance for co-ethnic immigrants from Eastern Europe and recently the Former Soviet Union. This article argues that changes to the way these privileged immigrant groups and their settlement are addressed should also reflect changes to the national idiom. The paper contrasts Greece to Germany and finds that, similarly to developments in its northern counterpart, Greek repatriates from the Former Soviet Union have been an important consequence of the ethno-cultural idiom and reinforced it at times. In the new millennium these immigrants’ importance is diminishing in reality, if not on paper. The article concludes that in spite of this and the citizenship reform of 2010, the tendency to see the country as a culturally homogeneous nation is still fairly strong in Greece. The analysis draws on interviews with ‘repatriates’ in both countries and with national policy-makers in Greece, as well as on newspaper clippings, opinion polls and statistical data, complemented by leading scholarship in the field to date.

  19. An Empirically Driven Time-Dependent Model of the Solar Wind

    Science.gov (United States)

    Linker, Jon A.; Caplan, Ronald M.; Downs, Cooper; Lionello, Roberto; Riley, Pete; Mikic, Zoran; Henney, Carl J.; Arge, Charles N.; Kim, Tae; Pogorelov, Nikolai

    2016-05-01

    We describe the development and application of a time-dependent model of the solar wind. The model is empirically driven, starting from magnetic maps created with the Air Force Data Assimilative Photospheric flux Transport (ADAPT) model at a daily cadence. Potential field solutions are used to model the coronal magnetic field, and an empirical specification is used to develop boundary conditions for an MHD model of the solar wind. The time-dependent MHD simulation shows classic features of stream structure in the interplanetary medium that are seen in steady-state models; it also shows time evolutionary features that do not appear in a steady-state approach. The model results compare reasonably well with 1 AU OMNI observations. Data gaps when SOLIS magnetograms were unavailable hinder the model performance. The reasonable comparisons with observations suggest that this modeling approach is suitable for driving long term models of the outer heliosphere. Improvements to the ingestion of magnetograms in flux transport models will be necessary to apply this approach in a time-dependent space weather model.

  20. High-Resolution Vector Magnetograms of the Sun's Poles from Hinode: Flux Distributions and Global Coronal Modeling

    Science.gov (United States)

    Petrie, Gordon

    2017-01-01

    The Sun's polar fields play a leading role in structuring the large-scale solar atmosphere and in determining the interplanetary magnetic field. They are also believed to supply the seed field for the subsequent solar activity cycle. However, present-day synoptic observations do not have sufficient spatial resolution or sensitivity to diagnose accurately the high-latitude magnetic vector field. The high spatial resolution and sensitivity of the full-Stokes observations from the Hinode Solar Optical Telescope Spectro-Polarimeter, observing the poles long-term, allows us to build up a detailed picture of the Cycle 24 polar field reversal, including the changing latitude distribution of the high-latitude flux, and to study the effect on global coronal field models. The Hinode observations provide detailed information on the dominant facular-scale magnetic structure of the polar fields, and their field inclination and flux distribution. Hybrid synoptic magnetograms are constructed from Hinode polar measurements and full-disk magnetograms from the Synoptic Optical Long-term Investigations of the Sun (SOLIS) Vector Spectro-Magnetograph (VSM), and coronal potential field models are calculated. Loss of effective spatial resolution at the highest latitudes presents complications. Possible improvements to synoptic polar data are discussed.

  1. Extensive sequencing of seven human genomes to characterize benchmark reference materials.

    Science.gov (United States)

    Zook, Justin M; Catoe, David; McDaniel, Jennifer; Vang, Lindsay; Spies, Noah; Sidow, Arend; Weng, Ziming; Liu, Yuling; Mason, Christopher E; Alexander, Noah; Henaff, Elizabeth; McIntyre, Alexa B R; Chandramohan, Dhruva; Chen, Feng; Jaeger, Erich; Moshrefi, Ali; Pham, Khoa; Stedman, William; Liang, Tiffany; Saghbini, Michael; Dzakula, Zeljko; Hastie, Alex; Cao, Han; Deikus, Gintaras; Schadt, Eric; Sebra, Robert; Bashir, Ali; Truty, Rebecca M; Chang, Christopher C; Gulbahce, Natali; Zhao, Keyan; Ghosh, Srinka; Hyland, Fiona; Fu, Yutao; Chaisson, Mark; Xiao, Chunlin; Trow, Jonathan; Sherry, Stephen T; Zaranek, Alexander W; Ball, Madeleine; Bobe, Jason; Estep, Preston; Church, George M; Marks, Patrick; Kyriazopoulou-Panagiotopoulou, Sofia; Zheng, Grace X Y; Schnall-Levin, Michael; Ordonez, Heather S; Mudivarti, Patrice A; Giorda, Kristina; Sheng, Ying; Rypdal, Karoline Bjarnesdatter; Salit, Marc

    2016-06-07

    The Genome in a Bottle Consortium, hosted by the National Institute of Standards and Technology (NIST) is creating reference materials and data for human genome sequencing, as well as methods for genome comparison and benchmarking. Here, we describe a large, diverse set of sequencing data for seven human genomes; five are current or candidate NIST Reference Materials. The pilot genome, NA12878, has been released as NIST RM 8398. We also describe data from two Personal Genome Project trios, one of Ashkenazim Jewish ancestry and one of Chinese ancestry. The data come from 12 technologies: BioNano Genomics, Complete Genomics paired-end and LFR, Ion Proton exome, Oxford Nanopore, Pacific Biosciences, SOLiD, 10X Genomics GemCode WGS, and Illumina exome and WGS paired-end, mate-pair, and synthetic long reads. Cell lines, DNA, and data from these individuals are publicly available. Therefore, we expect these data to be useful for revealing novel information about the human genome and improving sequencing technologies, SNP, indel, and structural variant calling, and de novo assembly.

  2. Solar Ca II K Observations

    Science.gov (United States)

    Bertello, Luca; Pevtsov, Alexei A.; Tlatov, Andrey; Singh, Jagdev

    2016-07-01

    Some of the most important archives of past and current long-term solar synoptic observations in the resonance line of Ca II K are described here. These observations are very important for understanding the state of the solar magnetism on time scales up to several decades. The first observations of this kind began in 1904 at the Kodaikanal Observatory (India), followed by similar programs at different other locations. Regular full-disk Ca II K monitoring programs started in 1915 at the Mount Wilson Observatory (USA) and in 1917 at the National Solar Observatory of Japan. Beginning in 1919 and in 1926 regular observations were taken also at the Paris-Meudon Observatory (France) and at the "Donati solar tower telescope of the Arcetri Astrophysical Observatory in Italy, respectively. In 1926 the the Astronomical Observatory of the Coimbra University in Portugal started its own program of Ca II K observations. Although some of these programs have been terminated over the years, their data archives constitute a unique resource for studies of solar variability. In the early 1970s, the National Solar Observatory (NSO) at Sacramento Peak (USA) started a new program of daily Sun-as-a-star observations in the Ca II K line. Today the NSO is continuing these observations through its Synoptic Optical Long-term Investigations of the Sun (SOLIS) facility.

  3. Correlation Between Sunspot Number and ca II K Emission Index

    CERN Document Server

    Bertello, Luca; Tlatov, Andrey; Singh, Jagdev

    2016-01-01

    Long-term synoptic observations in the resonance line of Ca II K constitute a fundamental database for a variety of retrospective analyses of the state of the solar magnetism. Synoptic Ca II K observations began in late 1904 at the Kodaikanal Observatory, in India. In early 1970s, the National Solar Observatory (NSO) at Sacramento Peak (USA) started a new program of daily Sun-as-a-star observations in the Ca II K line. Today the NSO is continuing these observations through its Synoptic Optical Long-term Investigations of the Sun (SOLIS) facility. These different data sets can be combined into a single disk-integrated Ca II K index time series that describes the average properties of the chromospheric emission over several solar cycles. We present such a Ca II K composite and discuss its correlation with the new entirely revised sunspot number data series. For this preliminary investigation, the scaling factor between pairs of time series was determined assuming a simple linear model for the relationship betwe...

  4. Correlation Between Sunspot Number and Ca ii K Emission Index

    Science.gov (United States)

    Bertello, Luca; Pevtsov, Alexei; Tlatov, Andrey; Singh, Jagdev

    2016-11-01

    Long-term synoptic observations in the resonance line of Ca ii K constitute a fundamental database for a variety of retrospective analyses of the state of the solar magnetism. Synoptic Ca ii K observations began in late 1904 at the Kodaikanal Observatory in India. In the early 1970s, the National Solar Observatory (NSO) at Sacramento Peak (USA) started a new program of daily Sun-as-a-star observations in the Ca ii K line. Today the NSO is continuing these observations through its Synoptic Optical Long-term Investigations of the Sun (SOLIS) facility. These different data sets can be combined into a single disk-integrated Ca ii K index time series that describes the average properties of the chromospheric emission over several solar cycles. We present such a Ca ii K composite and discuss its correlation with the new entirely revised sunspot number data series. For this preliminary investigation, the scaling factor between pairs of time series was determined assuming a simple linear model for the relationship between the monthly mean values during the duration of overlapping observations.

  5. High-throughput sequencing of black pepper root transcriptome

    Directory of Open Access Journals (Sweden)

    Gordo Sheila MC

    2012-09-01

    Full Text Available Abstract Background Black pepper (Piper nigrum L. is one of the most popular spices in the world. It is used in cooking and the preservation of food and even has medicinal properties. Losses in production from disease are a major limitation in the culture of this crop. The major diseases are root rot and foot rot, which are results of root infection by Fusarium solani and Phytophtora capsici, respectively. Understanding the molecular interaction between the pathogens and the host’s root region is important for obtaining resistant cultivars by biotechnological breeding. Genetic and molecular data for this species, though, are limited. In this paper, RNA-Seq technology has been employed, for the first time, to describe the root transcriptome of black pepper. Results The root transcriptome of black pepper was sequenced by the NGS SOLiD platform and assembled using the multiple-k method. Blast2Go and orthoMCL methods were used to annotate 10338 unigenes. The 4472 predicted proteins showed about 52% homology with the Arabidopsis proteome. Two root proteomes identified 615 proteins, which seem to define the plant’s root pattern. Simple-sequence repeats were identified that may be useful in studies of genetic diversity and may have applications in biotechnology and ecology. Conclusions This dataset of 10338 unigenes is crucially important for the biotechnological breeding of black pepper and the ecogenomics of the Magnoliids, a major group of basal angiosperms.

  6. Fermi-Pasta-Ulam recurrence and modulation instability

    CERN Document Server

    Kuznetsov, E A

    2016-01-01

    We give a qualitative explanation of the analog of the Fermi-Pasta-Ulam (FPU) recurrence in a one-dimensional focusing nonlinear Schrodinger equation (NLSE). That recurrence can be considered as a result of the nonlinear development of modulation instability. All known exact localized solitons-type solutions describing propagation on the background of the modulationally unstable condensate show the recurrence to the condensate state after its interaction with solitons. The condensate state locally recovers its original form with the same amplitude but a different phase after soliton leave its initial region. This is the analog of the FPU recurrence for the NLSE. Based on the integrability of the NLSE, we demonstrate that the FPU recurrence takes place not only for condensate but for more general solution in the form of the cnoidal wave. This solution is periodic in space and can be represented as a solitonic lattice. That lattice reduces to isolated soliton solution in the limit of large distance between soli...

  7. Breaking the 1000-gene barrier for Mimivirus using ultra-deep genome and transcriptome sequencing

    Directory of Open Access Journals (Sweden)

    Claverie Jean-Michel

    2011-03-01

    Full Text Available Abstract Background Mimivirus, a giant dsDNA virus infecting Acanthamoeba, is the prototype of the mimiviridae family, the latest addition to the family of the nucleocytoplasmic large DNA viruses (NCLDVs. Its 1.2 Mb-genome was initially predicted to encode 917 genes. A subsequent RNA-Seq analysis precisely mapped many transcript boundaries and identified 75 new genes. Findings We now report a much deeper analysis using the SOLiD™ technology combining RNA-Seq of the Mimivirus transcriptome during the infectious cycle (202.4 Million reads, and a complete genome re-sequencing (45.3 Million reads. This study corrected the genome sequence and identified several single nucleotide polymorphisms. Our results also provided clear evidence of previously overlooked transcription units, including an important RNA polymerase subunit distantly related to Euryarchea homologues. The total Mimivirus gene count is now 1018, 11% greater than the original annotation. Conclusions This study highlights the huge progress brought about by ultra-deep sequencing for the comprehensive annotation of virus genomes, opening the door to a complete one-nucleotide resolution level description of their transcriptional activity, and to the realistic modeling of the viral genome expression at the ultimate molecular level. This work also illustrates the need to go beyond bioinformatics-only approaches for the annotation of short protein and non-coding genes in viral genomes.

  8. Performance comparison of digital microRNA profiling technologies applied on human breast cancer cell lines.

    Directory of Open Access Journals (Sweden)

    Erik Knutsen

    Full Text Available MicroRNA profiling represents an important first-step in deducting individual RNA-based regulatory function in a cell, tissue, or at a specific developmental stage. Currently there are several different platforms to choose from in order to make the initial miRNA profiles. In this study we investigate recently developed digital microRNA high-throughput technologies. Four different platforms were compared including next generation SOLiD ligation sequencing and Illumina HiSeq sequencing, hybridization-based NanoString nCounter, and miRCURY locked nucleic acid RT-qPCR. For all four technologies, full microRNA profiles were generated from human cell lines that represent noninvasive and invasive tumorigenic breast cancer. This study reports the correlation between platforms, as well as a more extensive analysis of the accuracy and sensitivity of data generated when using different platforms and important consideration when verifying results by the use of additional technologies. We found all the platforms to be highly capable for microRNA analysis. Furthermore, the two NGS platforms and RT-qPCR all have equally high sensitivity, and the fold change accuracy is independent of individual miRNA concentration for NGS and RT-qPCR. Based on these findings we propose new guidelines and considerations when performing microRNA profiling.

  9. Aprotické elektrolyty pro superkondenzátory

    OpenAIRE

    2010-01-01

    Diplomová práce ověřuje vhodnost použití různých typů aprotických elektrolytů pro superkondenzátory, využívá metody impedanční spektroskopie k měření měrné iontové vodivosti a cyklické voltametrie k určení velikosti potenciálových oken. Použitá aprotická rozpouštědla - propylenkarbonát, dimethylsulfoxid, N, N dimethylformamid, ethylenkarbonát, dimethylkarbonát, diethylkarbonát a acetonitril. Do rozpouštědel byly přidány soli - LiClO4, NaClO4, KClO4, LiBF4, LiPF6, TEABF4 a TMABF4, za vzniku ro...

  10. La fine della sublimazione del lucro (scuole paritarie ed enti ecclesiastici

    Directory of Open Access Journals (Sweden)

    Nicola Colaianni

    2016-03-01

    Full Text Available "A CHIARE LETTERE” - TRANSIZIONI" • La fine della sublimazione del lucro (scuole paritarie ed enti ecclesiastici, di Nicola ColaianniLa giustizia amministrativa in materia scolastica ha una storia ultradecennale d'interpretazioni, talvolta anche acrobatiche, pro domo ecclesiae: dalle sentenze di fine anni ottanta – per cassare le quali bisognò scomodare la Corte Costituzionale – sulla trasformazione del diritto degli studenti di non avvalersi dell’insegnamento di religione cattolica in obbligo di svolgimento di un’attività alternativa a quelle più recenti sull’ammissibilità di un credito scolastico riservato ai soli avvalentisi e sul carattere culturale delle visite pastorali dei vescovi alle scuole. In questi, e in altri, casi il senso “fatto palese dal significato proprio delle parole secondo la connessione di esse” (diritto non può essere un obbligo, la scelta di non avvalersi non può avere effetti discriminanti, pastorale è la cura dei fedeli e quindi attiene alla religione e al culto non alla cultura non ha orientato l’interpretazione della legge nuova. A orientarla è stato il, sempre più mitico a uno sguardo spassionato della più recente legislazione, criterio di specialità, ...

  11. Il rapporto tra laicità e neutralità: una questione concettuale?

    Directory of Open Access Journals (Sweden)

    Corrado Del Bò

    2014-10-01

    Full Text Available Per la cortesia dell’Autore e dell’Editore il testo riproduce - tranne il paragrafo introduttivo, e con i soli aggiustamenti formali necessari a renderlo saggio autonomo - il capitolo 5 del volume monografico La neutralità necessaria. Liberalismo e religione nell’età del pluralismo(ETS Edizioni, Pisa, 2014.SOMMARIO: 1. Introduzione - 2. Su alcuni equivoci nel dibattito italiano sulla laicità - 3. Laicità e neutralità: un rapporto complicato - 4. La laicità della Corte costituzionale - 5. Conclusioni.ABSTRACT: This article, which is the chapter five of the book La neutralità necessaria. Liberalismo e religione nell’età del pluralismo (ETS, 2014, tries to identify what is the link between the idea of neutrality and secularism, suggesting that this link depends on the various possible interpretations of neutrality. Moreover, it is argued that we should avoid to reduce the issue to a nominal disputation about the meaning of the words and move our attention to the more fundamental problem about the relationship between politics and religion in a secular State.

  12. Diagnosis of CCSVI in Meniere syndrome.

    Science.gov (United States)

    Bruno, Aldo; Quarto, Gennaro; Califano, Luigi; Mastrangelo, Diego; De Vizia, Marcella; Salafia, Francesca; Bernardo, Benedetto

    In questo lavoro gli autori hanno valutato la possibilità di diagnosticare l’insufficienza venosa cronica cerebro-spinale (CCSVI) nei pazienti affetti da sindrome di Meniére resistente alle terapie mediche. Da aprile 2013 al luglio 2014 sono stati valutati 140 pazienti, 85 femmine, 55 maschi di età compresa tra 32 a 68 anni, con un’età media di 46 aa, affetti da sindrome di Meniere clinicamente definita secondo i criteri AAO 1995. I pazienti sono stati sottoposti ad un esame eco-colordoppler delle vene del collo e dei vasi venosi intracranici secondo il metodo di Zamboni. L’esame è stato eseguito anche in 100 pazienti sani, non affetti da malattia neurologica o audiovestibolare, di età simile a quella dei pazienti arruolati nello studio. L’incidenza della CCSVI, diagnosticata secondo il protocollo sviluppato dal Prof. Zamboni, nei pazienti con sindrome di Meniere è stata del 90%, con una presenza di lesioni più gravi sul lato interessato in casi unilaterali. Nella popolazione di controllo sono state rilevate anomalie compatibili con la CCSVI in soli tre pazienti (3%) comunque asintomatici. Vi è un’alta incidenza di CCSVI nei pazienti con malattia di Meniere clinicamente definita, in una percentuale tale da mettere in dubbio un evento casuale, soprattutto data la bassa prevalenza nel gruppo di controllo.

  13. Pantani di Marco Martinelli: una narrazione polifonica

    Directory of Open Access Journals (Sweden)

    Franco Nasi

    2014-05-01

    Full Text Available In questo articolo si analizza il testo drammaturgico Pantani di Marco Martinelli, una veglia epica teatrale di uno dei più noti eroi dello sport italiano degli ultimi anni. Il dramma rilegge anche la storia di Pantani e ricostruisce gli strani avvenimenti che portarono alla sospensione, alla estromissione del ciclista dal Giro d’Italia nel 1999, e in seguito alla sua profonda crisi psicologica e tragica morte in solitudine in un hotel, a soli 34 anni. Oltre alle modalità proprie della veglia rituale, il dramma utilizza anche i metodi del processo giudiziario, e offre testimonianze dettagliate che conducono a una riflessione critica sulla società italiana negli anni del potere politico di Berlusconi e della sua filosofia del consumismo. I tre diversi generi della retorica classica (epidittico, giudiziario e deliberativo si mescolano in questa complessa tessitura teatrale. Coniugando informazioni (processo e rito (veglia, Martinelli riesce a fondere la tragedia greca (forma mimetica e corale e il moderno teatro di narrazione (forma diegetica monologante. Combinando assieme scene intensamente drammatiche, ricordi di imprese sportive epiche, passaggi giocosi e parodici, e giustapponendo vari stili e registri (giornalistico, lirico, parodico, elegiaco, Martinelli crea momenti di forte “straniamento” e sorpresa, e rende particolarmente efficace il rito funebre, la ricostruzione storica della tragica vicenda di Pantani e la critica all’ideologia che ha dominato l’Italia negli anni di Berlusconi. 

  14. Resolving Azimuth Ambiguity Using Vertical Nature of Solar Quiet-Sun Magnetic Fields

    CERN Document Server

    Gosain, Sanjay

    2012-01-01

    The measurement of solar magnetic fields using the Zeeman effect diagnostics has a fundamental 180 degree ambiguity in the determination of the azimuth angle of the transverse field component. There are several methods that are used in the community and each one has its merits and demerits. Here we present a disambiguation idea that is based on the assumption that most of the magnetic field on the sun is predominantly vertical. While the method is not applicable to penumbra or other features harboring predominantly horizontal fields like the sheared neutral lines, it is useful for regions where fields are predominantly vertical like network and plage areas. The method is tested with the full-disk solar vector magnetograms observed by the VSM/SOLIS instrument. We find that statistically about 60-85 % of the pixels in a typical full-disk magnetogram has field inclination in the range of 0-30 degrees with respect to the local solar normal, and thus can be successfully disambiguated by the proposed method. Due to...

  15. Un Resultat Gravimetrique pour la Renaissance de la Theorie Corpusculaire "An Experimental Gravimetric Result for the Revival of Corpuscular Theory"

    CERN Document Server

    Duval, Maurice

    2005-01-01

    The gravitational phenomenon, founded on the assumption of absorption of a flux of gravitons through the matter, led to a law of attraction comprising a term of attenuation of gravity. The attenuation effect, which depends on the distribution of mass elements into the Sun, was compared with the relativistic effect for the orbital elements of planets. The calculations carried out with our modified law of Newton, lead to a perfect agreement for the advance of the perihelion of planets, and give an interaction cross section with the matter of 3.2x10^-17 m2/kg (approximately 10^-40 cm2/nucleon). The effect of attenuation during a solar eclipse will produce a local reduction in the soli-lunar attraction of 0.13 microgal. This small variation of gravity during the eclipse can lead to distortions of the geoid of about ten millimetres of which effect on gravity, estimated at a few microgals, is compatible with the observed gravitational anomalies. The gravimetric measurements taken in the area of Montreal during the ...

  16. A Solar Axion Search Using a Decommissioned LHC Test Magnet

    CERN Multimedia

    Raffelt, G; Lozza, V; Christensen, F E; Jakobsen, A C; Neff, S H; Dafni, T; Carmona martinez, J M; Giomataris, I; Krcmar, M; Vafeiadis, T; Luzon marco, G M; Gracia garza, J; Lakic, B; Solanki, S K; Ozbey, A; Davenport, M; Funk, W; Desch, K K; Laurent, J; Villar, J A; Jakovcic, K; Eleftheriadis, C; Cantatore, G; Diago ortega, A; Ortega ruiz, I; Papaevangelou, T; Zioutas, K; Gardikiotis, A; Cetin, S A; Hasinoff, M D; Hoffmann, D; Iguaz gutierrez, F J; Castel pablo, J F; Gninenko, S; Ferrer ribas, E; Liolios, A; Anastasopoulos, V; Kaminski, J; Garcia irastorza, I; Ruiz choliz, E; Krieger, C; Lutz, G; Fanourakis, G; Ruz armendariz, J; Vogel, J K; Pivovaroff, M J

    2002-01-01

    Previous solar axion searches have been carried out in Brookhaven (1990) and in Tokyo (2000- ), tracking the Sun with a dipole magnet. QCD inspired axions should be produced after the Big Bang, being thus candidates for the dark matter. The Sun is a very useful source of weakly interacting particles for fundamental research. Axions can be produced also in the Sun's core through the scattering of thermal photons in the Coulomb field of electric charges (Primakoff effect). In a transverse magnetic field the Primakoff effect can work in reverse, coherently converting the solar axions or other axion-like particles (ALPS) back into X-ray photons in the keV range. The conversion efficiency increases with $(B⋅L)^2$. In the CAST experiment an LHC prototype dipole magnet (B = 9 T and L = 10 m) with straight beam pipes provides a conversion efficiency exceeding that of the two earlier solar axion telescopes by almost a factor of 100. This magnet is mounted on a moving platform and coupled to both gas filled and soli...

  17. CORPORATE IDENTITY IN HANDLING A BUSINESS ENTITY

    Directory of Open Access Journals (Sweden)

    Margarita Mikhaylovna OZEROVA

    2015-01-01

    Full Text Available The paper explored urgency in seeking a personal identity for a corporate body. A Corporate identity gains in im-portance while upgrading the corporate culture. The corpo-rate identity consolidates the members of the staff for the sake of the common mission that makes for both a well-balanced development of an enterprise in economy terms and every member of the team, while facilitating awareness of the proper conduct required, i.e. showing respect, soli-darity and goodwill to the colleagues. The paper also speci-fied the properties, characteristics and basic duties of the corporate identity appearing for a an interface that is capa-ble not only of encouraging a productive activity but also helps employees realize their personal value for the com-mon cause. The study mentioned that even in case of one and only corporate identity, one can assume not only in-crease of labour productivity, but also some positive atti-tude changes in the employees to their professional duties performance, an increased interest in achieving jointly advancement of the company, and attitude changes in the scale of values and orientations.

  18. BIORREMEDIACIÓN DE UN SUELO CON DIESEL MEDIANTE EL USO DE MICROORGANISMOS AUTÓCTONOS

    Directory of Open Access Journals (Sweden)

    ARRIETA RAMÍREZ OLGA MARIA

    2012-07-01

    Full Text Available En este estudio, se aisló y caracterizó bioquímica y molecularmente un consorcio bacteriano capaz de degradar los diferentes hidrocarburos presentes en un combustible diesel,conformado por los siguientes géneros: Enterobacter sp, Bacillus sp, Staphylococcus aureus, Sanguibacter soli, Arthrobacter sp y Flavobacterium sp, a partir de un suelo contaminado con diesel a escala de laboratorio, y tratado mediante 2 tecnologías de biorremediación: atenuación natural y bioestimulación. Se definió como parámetro de control la concentración de Hidrocarburos Totales del Petróleo (HTP y para el cual, se obtuvo una reducción en la concentración en un periodo de 4 meses de 36,86% para atenuación natural y 50,99% para bioestimulación. La medición de la eficiencia de remoción de hidrocarburos se cuantificó por cromatografía de gases acoplada a masas (GC-MS.

  19. Theory and simulations of rotating convection

    CERN Document Server

    Barker, Adrian J; Lithwick, Yoram

    2014-01-01

    We study thermal convection in a rotating fluid, with the ultimate goal of explaining the structure of convection zones in rotating stars and planets. We first derive mixing-length theory for rapidly-rotating convection, arriving at the results of Stevenson (1979) via simple physical arguments. The theory predicts the properties of convection as a function of the imposed heat flux and rotation rate, independent of microscopic diffusivities. In particular, it predicts the mean temperature gradient; the rms velocity and temperature fluctuations; and the size of the eddies that dominate heat transport. We test all of these predictions with high resolution three-dimensional hydrodynamical simulations. The results agree remarkably well with the theory across more than two orders of magnitude in rotation rate. For example, the temperature gradient is predicted to scale as the rotation rate to the 4/5th power at fixed flux, and the simulations yield $0.75\\pm 0.06$. We conclude that the mixing length theory is a soli...

  20. Churches in Bologna

    Directory of Open Access Journals (Sweden)

    Giorgio Praderio

    2010-12-01

    Full Text Available È opportuno riconoscere, quando già non sia evidente, che la gran parte del patrimonio testimoniale architettonico ed artistico che il centro storico della nostra città può vantare, è costituito dalle chiese e dagli edifici ecclesiali, conventuali e religiosi. Bologna, tuttavia, è nata tardi alla pianificazione del turismo, e pare altrettanto restia ad aprirsi ad una gestione programmatica del territorio per la promozione di turismi culturali e consapevoli fuori dagli itinerari comuni, ormai asseverati storicamente. Si ha quasi l’impressione che si preferiscano lasciare tesori assoluti dell’arte quasi nascosti, patrimonio di pochi, dei soli iniziati che conoscono le vie d’accesso, le posizioni degli interruttori, gli eventuali custodi o gli orari di loro reperibilità: il patrimonio segreto della città si rivela così solo a chi ne possieda la combinazione, enfatizzando il raggiungimento di una meta quasi fosse una scoperta, come agli albori del “viaggio in Italia”.

  1. Application of Next Generation Sequencing Techniques in Food Microbiology%新一代测序技术在食品微生物学中的应用

    Institute of Scientific and Technical Information of China (English)

    韩齐; 李媛媛; 孙方达; 孔保华; 陈倩

    2016-01-01

    近年来,焦磷酸测序、SOLiD测序等一类新型测序技术引起了人们广泛的关注,这些技术统称为新一代测序(Next generation sequencing,NGS)技术.与第一代Sanger测序技术相比,NGS测序技术具有检测通量更高、用时更少、准确度更高以及检测费用更低等优点.序列的检测可以用于精确的鉴别微生物菌群,包括不可培养的生物体和含量极少的微生物,NGS技术的研发给微生物学研究带来了巨大的变革.通过对NGS系统和平台的介绍,综述了NGS技术在食品微生物学和食品发酵研究中的应用,并探讨了它们的局限性和发展前景,该技术的应用可以增加对微生物多样性、菌群结构和微生物数量动态变化的研究,从而提高对食品(主要是发酵食品)的监控.

  2. Digital PCR provides sensitive and absolute calibration for high throughput sequencing

    Directory of Open Access Journals (Sweden)

    Fan H Christina

    2009-03-01

    Full Text Available Abstract Background Next-generation DNA sequencing on the 454, Solexa, and SOLiD platforms requires absolute calibration of the number of molecules to be sequenced. This requirement has two unfavorable consequences. First, large amounts of sample-typically micrograms-are needed for library preparation, thereby limiting the scope of samples which can be sequenced. For many applications, including metagenomics and the sequencing of ancient, forensic, and clinical samples, the quantity of input DNA can be critically limiting. Second, each library requires a titration sequencing run, thereby increasing the cost and lowering the throughput of sequencing. Results We demonstrate the use of digital PCR to accurately quantify 454 and Solexa sequencing libraries, enabling the preparation of sequencing libraries from nanogram quantities of input material while eliminating costly and time-consuming titration runs of the sequencer. We successfully sequenced low-nanogram scale bacterial and mammalian DNA samples on the 454 FLX and Solexa DNA sequencing platforms. This study is the first to definitively demonstrate the successful sequencing of picogram quantities of input DNA on the 454 platform, reducing the sample requirement more than 1000-fold without pre-amplification and the associated bias and reduction in library depth. Conclusion The digital PCR assay allows absolute quantification of sequencing libraries, eliminates uncertainties associated with the construction and application of standard curves to PCR-based quantification, and with a coefficient of variation close to 10%, is sufficiently precise to enable direct sequencing without titration runs.

  3. Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions.

    Science.gov (United States)

    Savelyeva, Anna V; Kuligina, Elena V; Bariakin, Dmitry N; Kozlov, Vadim V; Ryabchikova, Elena I; Richter, Vladimir A; Semenov, Dmitry V

    2017-01-01

    Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being internalized, possess the ability to modulate vital processes in recipient cells. In this study, with SOLiD sequencing technology, we performed identification, classification, and quantification of RNAs from blood fractions: cells, plasma, plasma vesicles pelleted at 16,000g and 160,000g, and vesicle-depleted plasma supernatant of healthy donors and non-small cell lung cancer (NSCLC) patients. It was determined that 16,000g blood plasma vesicles were enriched with cell-free mitochondria and with a set of mitochondrial RNAs. The variable RNA set of blood plasma 160,000g pellets reflected the prominent contribution of U1, U5, and U6 small nuclear RNAs' fragments and at the same time was characterized by a remarkable depletion of small nucleolar RNAs. Besides microRNAs, the variety of fragments of mRNAs and snoRNAs dominated in the set of circulating RNAs differentially expressed in blood fractions of NSCLC patients. Taken together, our data emphasize that not only extracellular microRNAs but also circulating fragments of messenger and small nuclear/nucleolar RNAs represent prominent classes of circulating regulatory ncRNAs as well as promising circulating biomarkers for the development of disease diagnostic approaches.

  4. Comparison of Ground- and Space-based Longitudinal Magnetograms

    CERN Document Server

    Pietarila, A; Harvey, J W; Pevtsov, A A

    2012-01-01

    We compare photospheric line-of-sight magnetograms from the Synoptic Long-term Investigations of the Sun (SOLIS) vector spectromagnetograph (VSM) instrument with observations from the 150-foot Solar Tower at Mt. Wilson (MWO), Helioseismic and Magnetic Imager (HMI) on Solar Dynamics Observatory (SDO), and Michelson Doppler Imager (MDI) on Solar and Heliospheric Observatory (SOHO). We find very good agreement between VSM and the other data sources for both disk-averaged flux densities and pixel-by-pixel measurements. We show that the VSM mean flux density time series is of consistently high signal-to-noise with no significant zero-offsets. We discuss in detail some of the factors -spatial resolution, flux dependence and position on the solar disk- affecting the determination of scaling between VSM and SOHO/MDI or SDO/HMI magnetograms. The VSM flux densities agree well with spatially smoothed data from MDI and HMI, although the scaling factors show clear dependence on flux density. The factor to convert VSM to H...

  5. Testing an astronomically-based decadal-scale empirical harmonic climate model versus the IPCC (2007) general circulation climate models

    CERN Document Server

    Scafetta, Nicola

    2012-01-01

    We compare the performance of a recently proposed empirical climate model based on astronomical harmonics against all available general circulation climate models (GCM) used by the IPCC (2007) to interpret the 20th century global surface temperature. The proposed model assumes that the climate is resonating with, or synchronized to a set of natural harmonics that have been associated to the solar system planetary motion, mostly determined by Jupiter and Saturn. We show that the GCMs fail to reproduce the major decadal and multidecadal oscillations found in the global surface temperature record from 1850 to 2011. On the contrary, the proposed harmonic model is found to well reconstruct the observed climate oscillations from 1850 to 2011, and it is able to forecast the climate oscillations from 1950 to 2011 using the data covering the period 1850-1950, and vice versa. The 9.1-year cycle is shown to be likely related to a decadal Soli/Lunar tidal oscillation, while the 10-10.5, 20-21 and 60-62 year cycles are sy...

  6. Calcite-forming bacteria for compressive strength improvement in mortar.

    Science.gov (United States)

    Park, Sung-Jin; Park, Yu-Mi; Chun, Woo-Young; Kim, Wha-Jung; Ghim, Sa-Youl

    2010-04-01

    Microbiological calcium carbonate precipitation (MCP) has been investigated for its ability to improve the compressive strength of concrete mortar. However, very few studies have been conducted on the use of calcite-forming bacteria (CFB) to improve compressive strength. In this study, we discovered new bacterial genera that are capable of improving the compressive strength of concrete mortar. We isolated 4 CFB from 7 environmental concrete structures. Using sequence analysis of the 16S rRNA genes, the CFB could be partially identified as Sporosarcina soli KNUC401, Bacillus massiliensis KNUC402, Arthrobacter crystallopoietes KNUC403, and Lysinibacillus fusiformis KNUC404. Crystal aggregates were apparent in the bacterial colonies grown on an agar medium. Stereomicroscopy, scanning electron microscopy, and x-ray diffraction analyses illustrated both the crystal growth and the crystalline structure of the CaCO3 crystals. We used the isolates to improve the compressive strength of concrete mortar cubes and found that KNUC403 offered the best improvement in compressive strength.

  7. Comparing Coronal and Heliospheric Magnetic Fields over Several Solar Cycles

    Science.gov (United States)

    Koskela, J. S.; Virtanen, I. I.; Mursula, K.

    2017-01-01

    Here we use the PFSS model and photospheric data from Wilcox Solar Observatory, SOHO/MDI, SDO/HMI, and SOLIS to compare the coronal field with heliospheric magnetic field measured at 1 au, compiled in the NASA/NSSDC OMNI 2 data set. We calculate their mutual polarity match and the power of the radial decay, p, of the radial field using different source surface distances and different number of harmonic multipoles. We find the average polarity match of 82% for the declining phase, 78%–79% for maxima, 76%–78% for the ascending phase, and 74%–76% for minima. On an average, the source surface of 3.25 RS gives the best polarity match. We also find strong evidence for solar cycle variation of the optimal source surface distance, with highest values (3.3 RS) during solar minima and lowest values (2.6 RS–2.7 RS) during the other three solar cycle phases. Raising the number of harmonic terms beyond 2 rarely improves the polarity match, showing that the structure of the HMF at 1 au is most of the time rather simple. All four data sets yield fairly similar polarity matches. Thus, polarity comparison is not affected by photospheric field scaling, unlike comparisons of the field intensity.

  8. Long-term Synoptic Observations of Ca II K and Magnetic Flux

    Science.gov (United States)

    Bertello, L.; Pevtsov, A. A.; Tlatov, A. G.

    2016-04-01

    Long-term synoptic observations in the resonance line of Ca II K and measurements of the solar magnetic flux over several decades constitute a fundamental database for a variety of retrospective analyzes of the state of the solar magnetism. These data archives may also hold the key for untangling some of the mysteries behind the solar dynamo, which in turn could result in a better predictive capability of current dynamo models. Synoptic Ca II K observations began in 1907 at the Kodaikanal observatory (India) and in 1915 at the Mt Wilson (California, USA) solar observatory. By the early 1970s a number of synoptic programs for solar magnetic observations were established that provided full disk magnetograms. These programs include measurements carried out at the Mt Wilson 150-foot solar tower, Wilcox observatory (California, USA), and by the National Solar Observatory (NSO, USA). Today the NSO is continuing these observations through its Synoptic Optical Long-term Investigations of the Sun (SOLIS) facility. We will review some of these historical observations, their properties, and their importance for understanding the behavior of the solar magnetic field over multidecadal time scales. We will also show recent results about using Ca II K spectroheliograms and sunspot magnetic field measurements to reconstruct homogeneous series of pseudo-magnetograms prior the magnetograph era.

  9. Preparation of radiolabeled trichothecenes, simple trichothecenes for generation of generic antibodies, and a study of the fate of trichothecenes in soil. Final report, 1 September 1985-1 November 1986

    Energy Technology Data Exchange (ETDEWEB)

    Jarvis, B.B.

    1987-01-12

    The objectives of this research fell into three areas: (1) preparation of radiolabeled macrocylic trichothecenes and their subsequent use in studies of trichothecene metabolism in mammalian systems; (2) preparation of a series of modified trichothecenes which will be useful in the preparation of generic antibodies for the simple trichothecenes; and (3) study of the transport in solis and plants by the trichothecenes, in order to determine the long-term ecological effects of trichothecenes contamination of highly contaminated areas. 15-3H-verrucarol was prepared by two different synthetic procedures. Verrucarin A was labeled with tritium to give 2'-3H-verrucarin A and 16-3H-verrucarin A. Two nontoxic trichothecenes were prepared, one of which has a 4,15-ether bridge and the other has an 8,15-ether bridge. Roridin A was shown to bind irreversibly to soils, with the percent recovery from spiked-soil sample decreasing with time and decreasing with decreasing concentration of mycotoxin in the soil. The potent cytotoxin myrotoxin B was isolated from a fungal culture and submitted for toxicological evaluation.

  10. Il punto su Sebastijan Krelj

    Directory of Open Access Journals (Sweden)

    Neva Godini

    1987-12-01

    Full Text Available Come Sebastianus  Krelo Austriacus si era iscritto all'Accademia  di Jena nel 1557 colui che oggi viene considerato uno dei protagonisti di spicco dell'epopea pro­ testante slovena. Sono ormai parecchi anni che, nel celebrare l'anniversario del1584 nella cultura e storia slovena (anno della pubblicazione della traduzione della Bibbia ad opera di Jurij Dalmatin e della prima grammatica slovena, scritta in Iatino, Arti­ cae horulae di Adam Bohorič, non si contano ormai più libri, saggi, articoli, con­ vegni e commemorazioni varie così in Slovenia come all'estero  ed in particolare a Derendingen, oggi periferia della città di Tübingen, che diventò Ia seconda patria di colui che viene considerato il padre della letteratura slovena, cioè Primož Trubar.  E nella vicina città di Urach aveva sede l'importantissima tipografia dei protestanti sloveni e non: basterà ricordare che qui in soli cinque anni vennero alia stampa ben 50 tra pubblicazioni in sloveno, croato, glagolitico, italiano ecc. Ma queste dovreb­ bero essere cose abbastanza  risapute. Meno conosciuta e, oseremmo dire quasi un po' trascurata   in questa messe di commemorazioni, appare l'opera  e l'importanza di uno dei protagonisti del protestantesimo sloveno, appunto  Sebastijan Krelj.

  11. Star-Paths, Stones and Horizon Astronomy

    Science.gov (United States)

    Brady, Bernadette

    2015-05-01

    Archaeoastronomers tend to approach ancient monuments focusing on the landscape and the horizon calendar events of sun and moon and, due to problems with precession, generally ignore the movement of the stars. However, locating the position of solar calendar points on the horizon can have other uses apart from calendar and/or cosmological purposes. This paper firstly suggests that the stars do not need to be ignored. By considering the evidence of the Phaenomena, a sky poem by Aratus of Soli, a third century BC Greek poet, and his use of second millennium BC star lore fragments, this paper argues that the stars were a part of the knowledge of horizon astronomy. Aratus' poem implied that the horizon astronomy of the late Neolithic and Bronze Age periods included knowledge of star-paths or 'linear constellations' that were defined by particular horizon calendar events and other azimuths. Knowledge of such star-paths would have enabled navigation and orientation, and by using permanent markers, constructed or natural, to define these paths, they were immune to precession as the stones could redefine a star-path for a future generation. Finally the paper presents other possible intentions behind the diverse orientation of passage tombs and some megalithic sites.

  12. VizieR Online Data Catalog: Scheiner drawing sunspot areas and tilt angles (Arlt+, 2016)

    Science.gov (United States)

    Arlt, R.; Senthamizh Pavai, V.; Schmiel, C.; Spada, F.

    2016-09-01

    Christoph Scheiner and his collaborators observed the sunspots from 1611-1631 at five different locations of Rome in Italy, Ingolstadt in Germany, Douai (Duacum in Latin) in France, Freiburg im Breisgau, Germany and Vienna, Austria. However, most of his published drawings were made in Rome. These sunspot drawings are important because they can tell us how the solar activity declined to a very low-activity phase which lasted for nearly five decades. The three sources used for the sunspot data extraction are Scheiner (1630rour.book.....S, Rosa Ursina sive solis), Scheiner (1651ppsm.book.....S, Prodromus pro sole mobili et terra stabili contra Academicum Florentinum Galilaeum a Galilaeis), and Reeves & Van Helden (2010, On sunspots. Galileo Galilei and Christoph Scheiner (University of Chicago Press)). The suspot drawings show the sunspot groups traversing the solar disk in a single full-disk drawing. The positions and areas of the sunspots were measured using 13 circular cursor shapes with different diameters. Umbral areas for 8167 sunspots and tilt angles for 697 manually selected, supposedly bipolar groups were obtained from Scheiner's sunspot drawings. The database does not contain spotless days. There is, of course, no polarity information in the sunspot drawings, so the tilt angles are actually pseudo-tilt angles. Both an updated sunspot database and a tilt angle database may be available at http://www.aip.de/Members/rarlt/sunspots for further study. (2 data files).

  13. Systematic functional analysis and application of a cold-active serine protease from a novel Chryseobacterium sp.

    Science.gov (United States)

    Mageswari, Anbazhagan; Subramanian, Parthiban; Chandrasekaran, Suganthi; Karthikeyan, Sivashanmugam; Gothandam, Kodiveri Muthukaliannan

    2017-02-15

    Psychrotolerant bacteria isolated from natural and artificially cold environments were screened for synthesis of cold-active protease. The strain IMDY showing the highest protease production at 5°C was selected and phylogenetic analysis revealed that IMDY as novel bacterium with Chryseobacterium soli(T) as its nearest neighbor. Classical optimization enhanced the protease production from 18U/mg to 26U/mg and the enzyme was found to be active at low temperature, activity enhanced by CaCl2, inhibited by PMSF, stable against NaCl, and its activity retained in the presence of surfactants, organic solvents and detergents. On testing, the meat tenderization, myofibril fragmentation, pH, and TBA values were favorable in IMDY-protease treated meat compared to control. SDS profiling and SEM analysis also showed tenderization in meat samples. Hence, this study proposes to consider the cold-active protease from Chryseobacterium sp. IMDY as a pertinent candidate to develop potential applications in food processing industry.

  14. SRAdb: query and use public next-generation sequencing data from within R

    Directory of Open Access Journals (Sweden)

    Zhu Yuelin

    2013-01-01

    Full Text Available Abstract Background The Sequence Read Archive (SRA is the largest public repository of sequencing data from the next generation of sequencing platforms including Illumina (Genome Analyzer, HiSeq, MiSeq, .etc, Roche 454 GS System, Applied Biosystems SOLiD System, Helicos Heliscope, PacBio RS, and others. Results SRAdb is an attempt to make queries of the metadata associated with SRA submission, study, sample, experiment and run more robust and precise, and make access to sequencing data in the SRA easier. We have parsed all the SRA metadata into a SQLite database that is routinely updated and can be easily distributed. The SRAdb R/Bioconductor package then utilizes this SQLite database for querying and accessing metadata. Full text search functionality makes querying metadata very flexible and powerful. Fastq files associated with query results can be downloaded easily for local analysis. The package also includes an interface from R to a popular genome browser, the Integrated Genomics Viewer. Conclusions SRAdb Bioconductor package provides a convenient and integrated framework to query and access SRA metadata quickly and powerfully from within R.

  15. Next-generation sequencing technologies and the application in microbiology-A review%高通量测序技术及其在微生物学研究中的应用

    Institute of Scientific and Technical Information of China (English)

    秦楠; 栗东芳; 杨瑞馥

    2011-01-01

    20世纪70年代发明的核酸测序技术为基因组学及其相关学科的发展做出了巨大贡献,本世纪初发展的以Illumina公司的HiSeq 2000,ABI公司的SOLID,和Roche公司的454技术为代表的高通量测序技术又为基因组学的发展注入了新活力.本文在阐述这些技术的基础上,着重讨论了新一代测序技术在微生物领域中的应用.%Since its invention in 1970s, nucleic acid sequencing technology has contributed tremendously to the genomics advances.The next-generation sequencing technologies, represented by HiSeq 2000 from Illumina, SOLiD from Applied Biosystems and 454 from Roche, re-energized the application of genomics.In this review, we first introduced the next-generation sequencing technologies, then, described their potential applications in the field of microbiology.

  16. The dazed and confused identity of Agassiz’s land tortoise, Gopherus agassizii (Testudines, Testudinidae) with the description of a new species, and its consequences for conservation

    Science.gov (United States)

    Murphy, Robert W.; Berry, Kristin H.; Edwards, Taylor; Leviton, Alan E.; Lathrop, Amy; Riedle, J. Daren

    2011-01-01

    Abstract We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii (Cooper). The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of Gopherus lepidocephalus (Ottley & Velázques Solis. 1989) from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA, and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because Gopherus lepidocephalus is from the Mojavian population, no names are available for the Sonoran species. Thus, a new species, Gopherus morafkai sp. n., is named and this action reduces the distribution of Gopherus agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of Gopherus agassizii, which may deserve a higher level of protection. PMID:21976992

  17. The dazed and confused identity of Agassiz’s land tortoise, Gopherus agassizii (Testudines: Testudinidae with the description of a new species and its consequences for conservation

    Directory of Open Access Journals (Sweden)

    Robert Murphy

    2011-06-01

    Full Text Available We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii Cooper. The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of G. lepidocephalus Ottley et Velázques Solis, 1989 from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because G. lepidocephalus is from the Mojavian population no names are available for the Sonoran species. Thus, a new species, Gopherus morafkai sp. n., is named and this action reduces the distribution of G. agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of G. agassizii, which may deserve a higher level of protection.

  18. The dazed and confused identity of Agassiz's land tortoise, Gopherus agassizii (Testudines, Testudinidae) with the description of a new species, and its consequences for conservation.

    Science.gov (United States)

    Murphy, Robert W; Berry, Kristin H; Edwards, Taylor; Leviton, Alan E; Lathrop, Amy; Riedle, J Daren

    2011-01-01

    We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii (Cooper). The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of Gopherus lepidocephalus (Ottley & Velázques Solis. 1989) from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA, and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because Gopherus lepidocephalus is from the Mojavian population, no names are available for the Sonoran species. Thus, a new species, Gopherus morafkaisp. n., is named and this action reduces the distribution of Gopherus agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of Gopherus agassizii, which may deserve a higher level of protection.

  19. microRNAs control of in vivo toxicity from graphene oxide in Caenorhabditis elegans.

    Science.gov (United States)

    Wu, Qiuli; Zhao, Yunli; Zhao, Gui; Wang, Dayong

    2014-10-01

    The molecular basis for in vivo graphene oxide (GO) toxicity is still largely unclear. We here used Caenorhabditis elegans to investigate the microRNAs (miRNAs) control of GO toxicity. With the aid of SOLiD sequencing, we identified 23 up-regulated and 8 down-regulated miRNAs in GO-exposed nematodes. Gene ontology and KEGG pathway database analysis implied that these identified miRNAs might be involved in control of many biological processes, and some of them suggest the possible new functions of GO. Functions of the identified miRNAs in regulating the GO toxicity on lifespan were confirmed in the available miRNAs mutants. Moreover, we provide the evidence to raise a hypothesis that GO may reduce lifespan through influencing the functions of insulin/IGF signaling, TOR signaling, and germline signaling pathways controlled by miRNAs. Our results will be helpful for understanding the molecular basis for GO toxicity, and finding clues for useful surface modifications to reduce GO toxicity. From the clinical editor: In this study, toxicity of graphene oxide is studied in a Caenorhabditis elegans model via microRNA analysis. The authors report that multiple important pathways are influenced by GO and raise a hypothesis that GO may reduce lifespan through influencing the functions of insulin/IGF signaling, TOR signaling, and germline signaling pathways.

  20. Lunar soil simulant and synthesized nanoparticles of magnetite exhibit diverse neurotoxic potential

    Science.gov (United States)

    Borisova, Tatiana; Krisanova, Natalia; Sivko, Roman; Nazarova, Anastasiya; Borysov, Arseniy

    Lunar soli simulant can be deleterious to human physiology and the components of lunar soil may be internalized by lung epithelium and may overcome the blood-brain barrier. Nanoparticles of ferric oxide are one of the components of Lunar soil simulants. Neurotoxic potential of lunar soil simulant and synthesized nanoparticles of magnetite was analyzed. The size of particles, their effects on membrane potential, acidification of synaptic vesicles, uptake and ambient level of glutamate, which is the major excitatory neurotransmitter in the CNS, were studied in isolated rat brain nerve terminals (synaptosomes) using photon correlation spectroscopy, spectrofluorimetry, radiolabeled assay, respectively. No significant effect of Lunar soil simulant and synthesized nanoparticles of magnetite on acidification of synaptic vesicles were found in synaptosomes. Also, nanoparticles did not influence the potential of the plasma membrane of synaptosomes. Unspecific binding of L-[14C]glutamate to synaptosomes was not altered by nanoparticles of magnetite, whereas in the presence of Lunar soil simulant this parameter was changed. Thus, it was suggested that Lunar soil simulant might disturb glutamate homeostasis in the mammalian CNS.

  1. Interaction of nanoparticles of ferric oxide with brain nerve terminals and blood platelets

    Science.gov (United States)

    Borisova, Tatiana; Krisanova, Natalia; Sivko, Roman; Borisov, Arseniy

    2012-07-01

    Nanoparticles of ferric oxide are the components of Lunar and Martian soil simulants. The observations suggest that exposure to Lunar soli simulant can be deleterious to human physiology and the components of lunar soil may be internalized by lung epithelium and may overcome the blood-brain barrier. The study focused on the effects of nanoparticles of ferric oxide on the functional state of rat brain nerve terminals (synaptosomes) and rabbit blood platelets. Using photon correlation spectroscopy, we demonstrated the binding of nanoparticles of ferric oxide with nerve terminals and platelets. Nanoparticles did not depolarize the plasma membrane of nerve terminals and platelets that was shown by fluorimetry with potential-sensitive fluorescent dye rhodamine 6G. Using pH-sensitive fluorescent dye acridine orange, we revealed that the acidification of synaptic vesicles of nerve terminals and secretory granules of platelets did not change in the presence of nanoparticles. The initial velocity of uptake of excitatory neurotransmitter glutamate was not influenced by nanoparticles of ferric oxide, whereas glutamate binding to nerve terminals was altered. Thus, it was suggested that nanoparticles of ferric oxide might disturb glutamate transport in the mammalian CNS.

  2. Analysis of Solidiifcation of High Manganese Steels Using Improved Differential Thermal Analysis Method

    Institute of Scientific and Technical Information of China (English)

    Chang-ling ZHUANG; Jian-hua LIU; Christian BERNHARD; Peter PRESOLY

    2015-01-01

    High manganese steels can damage the differential thermal analysis (DTA) instrument due to the manganese evaporation during high temperature experiments. After analyzing the relationship between residual oxygen and manganese evaporation, tanta-lum metal was employed to modify the crucible of DTA, and zirconium getter together with strict gas puriifcation measures were applied to control the volatilization of manganese. By these modiifcations, problems of thermocouple damage and DTA instrument contamination were successfully resolved. Cobalt samples were adopted to calibrate the accuracy of DTA instruments under the same trial condition of high manganese steel samples, and the detection error was conifrmed to be less than 1 °C. Liquidus and soli-dus temperatures of high Mn steels were measured by improved DTA method. It was found that the liquidus temperatures of sam-ples tested by experiments increased linearly with the heating rates. To eliminate the effects of the heating rate, equilibrium liquidus temperature was determined by iftting the liquidus temperatures at different heating rates, and referred as real liquidus temperature. No clear relationship between solidus temperatures and heating rates was found, and the solidus temperature was ifnally set as the average value of several experimental data.

  3. A New Approach for Flexible Molecular Docking Based on Swarm Intelligence

    Directory of Open Access Journals (Sweden)

    Yi Fu

    2015-01-01

    Full Text Available Molecular docking methods play an important role in the field of computer-aided drug design. In the work, on the basis of the molecular docking program AutoDock, we present QLDock as a tool for flexible molecular docking. For the energy evaluation, the algorithm uses the binding free energy function that is provided by the AutoDock 4.2 tool. The new search algorithm combines the features of a quantum-behaved particle swarm optimization (QPSO algorithm and local search method of Solis and Wets for solving the highly flexible protein-ligand docking problem. We compute the interaction of 23 protein-ligand complexes and compare the results with those of the QDock and AutoDock programs. The experimental results show that our approach leads to substantially lower docking energy and higher docking precision in comparison to Lamarckian genetic algorithm and QPSO algorithm alone. QPSO-ls algorithm was able to identify the correct binding mode of 74% of the complexes. In comparison, the accuracy of QPSO and LGA is 52% and 61%, respectively. This difference in performance rises with increasing complexity of the ligand. Thus, the novel algorithm QPSO-ls may be used to dock ligand with many rotatable bonds with high accuracy.

  4. Determination of normative criteria and validation of the SKT for use in Spanish-speaking populations.

    Science.gov (United States)

    Ostrosky-Solís, F; Dávila, G; Ortiz, X; Vega, F; García Ramos, G; de Celis, M; Dávila, L; Gómez, C; Jiménez, S; Juárez, S; Corte, G; Molina, B

    1999-06-01

    Most of the tests, questionnaires, and neuropsychological batteries for the assessment of dementia have been translated and adapted for use in the Hispanic population without having normative data, which results in a high number of false positives when age and educational level are not considered. The Short Cognitive Performance Test (SKT) is a psychometric instrument evaluating memory and attention deficits that has been developed and standardized in Germany (Erzigkeit, 1989a, 1989b). The objective of this study was to adapt the SKT, to establish normative criteria that take into consideration age and educational level, and to establish its concurrent validity in comparison to other neuropsychological tests: Brief Neuropsychological Evaluation for Spanish-Speaking Subjects (Ostrosky-Solis et al., 1994), the Mini-Mental State Examination (Folstein et al., 1975), and the Blessed Functional Scale (Blessed et al., 1968) in 238 neurologically intact subjects and 97 subjects with mild to moderate dementia. The SKT showed adequate sensitivity (80.5%) and specificity (80.3%) in subjects with medium and high educational level; however, the sensitivity and specificity diminished (75% and 56.7%) in subjects with no education or low educational level. The adapted and validated version of the SKT in the Mexican population has been shown to be a psychometric instrument that in subjects with medium educational level can detect cognitive alterations and is able to determine the severity of deterioration; however, in subjects with low educational level and severe dementia, the SKT cannot be usefully administered.

  5. Sphingosinicella vermicomposti sp. nov., isolated from vermicompost, and emended description of the genus Sphingosinicella.

    Science.gov (United States)

    Yasir, Muhammad; Aslam, Zubair; Song, Geun Cheol; Jeon, Che Ok; Chung, Young Ryun

    2010-03-01

    A Gram-stain-negative, rod-shaped bacterium, designated strain YC7378(T) was isolated from vermicompost (VC) collected at Masan, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain YC7378(T) grew optimally at 30 degrees C and at pH 6.5-8.5. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC7378(T) belongs to the genus Sphingosinicella in the family Sphingomonadaceae. The most closely related strains are Sphingosinicella soli KSL-125(T) (95.7 %), Sphingosinicella xenopeptidilytica 3-2W4(T) (95.6 %) and Sphingosinicella microcystinivorans Y2(T) (95.5 %). Strain YC7378(T) contained ubiquinone Q-10 as the major respiratory quinone system and sym-homospermidine as the major polyamine. The major fatty acids of strain YC7378(T) were C(18 : 1)omega7c, C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, C(14 : 0) 2-OH and C(16 : 0). The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The total DNA G+C content was 59.4 mol%. The phenotypic, phylogenetic and chemotaxonomic data showed that strain YC7378(T) represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella vermicomposti sp. nov. is proposed. The type strain is YC7378(T) (=KCTC 22446(T) =DSM 21593(T)).

  6. Calvyn en die Calvinisme

    Directory of Open Access Journals (Sweden)

    B. J. Engelbrecht

    1988-01-01

    Full Text Available Calvin and Calvinism The theology of Calvin had and still has a worldwide influence. During the course of history, theologians suggested various themes as the central theme of Calvin's theology around which it is possible to structure all the aspects of his theological thought to a coherent system. It is a question if this is really possible writhout a lesser or greater measure of distortion of Calvin's own theological thought. In this article two 'central themes' are treated, that is the theme of the soli Deo gloria and the theme of the sola et tola Scriptura. In each case Calvin's own thinking on the subject concerned, is described and then the various interpretations in history of the theme concerned are treated. In this way trends in calvinistic thinking and groups such as Kuypers Neo-Calvinism, the Social Gospel, the rationalistic Orthodoxism, the methodistic Pietism, the Enlightenment, theological Liberalism and modem hermeneutics are broached. Finally, ensuing from this, the most important issues to be adrcssed presently in Calvinistic thought, are identified.

  7. Borders and Legal Criteria for Acquiring Nationality

    Directory of Open Access Journals (Sweden)

    María Elósegui Itxaso

    2008-09-01

    Full Text Available Legal criteria for acquiring nationality are crucial in the integration of persons into society, since they provide access to the right to vote. Until now, the criteria most frequently used are those of ius soli (birth and ius sanguinis (nationality is inherited from the parents, which comply with previous anthropological approaches and which jurists accept without reflection, or consider to be unshakeable traditions.The author’s proposal in this article is to accept that some of these legal criteria should be reformed, though not in an anarchic manner. On one hand, some of the ethnic criteria may be respected, but on the other, the processes of acquiring nationality should be streamlined by accepting the desire of persons wanting to change their nationality on moving to a new country of residence. Meanwhile, we must establish channels of demand for accepting the democratic values and legal system of the welcoming country, as a result of which it would be fair to call for a prior learning period before the rights to nationality and suffrage are granted. The author also adds – and accepts as being a fundamental element – some of Habermas’ inclusion theses, though she stresses that this discourse should be organised into two specific, feasible legal solutions or rather, in a realistic manner.

  8. Maximum power analysis of photovoltaic module in Ramadi city

    Directory of Open Access Journals (Sweden)

    Majid Shahatha Salim, Jassim Mohammed Najim, Salih Mohammed Salih

    2013-01-01

    Full Text Available Performance of photovoltaic (PV module is greatly dependent on the solar irradiance, operating temperature, and shading. Solar irradiance can have a significant impact on power output of PV module and energy yield. In this paper, a maximum PV power which can be obtain in Ramadi city (100km west of Baghdad is practically analyzed. The analysis is based on real irradiance values obtained as the first time by using Soly2 sun tracker device. Proper and adequate information on solar radiation and its components at a given location is very essential in the design of solar energy systems. The solar irradiance data in Ramadi city were analyzed based on the first three months of 2013. The solar irradiance data are measured on earth's surface in the campus area of Anbar University. Actual average data readings were taken from the data logger of sun tracker system, which sets to save the average readings for each two minutes and based on reading in each one second. The data are analyzed from January to the end of March-2013. Maximum daily readings and monthly average readings of solar irradiance have been analyzed to optimize the output of photovoltaic solar modules. The results show that the system sizing of PV can be reduced by 12.5% if a tracking system is used instead of fixed orientation of PV modules.

  9. Semi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms

    Directory of Open Access Journals (Sweden)

    Eveline Farias-Hesson

    2010-01-01

    Full Text Available Next-generation sequencing platforms are powerful technologies, providing gigabases of genetic information in a single run. An important prerequisite for high-throughput DNA sequencing is the development of robust and cost-effective preprocessing protocols for DNA sample library construction. Here we report the development of a semi-automated sample preparation protocol to produce adaptor-ligated fragment libraries. Using a liquid-handling robot in conjunction with Carboxy Terminated Magnetic Beads, we labeled each library sample using a unique 6 bp DNA barcode, which allowed multiplex sample processing and sequencing of 32 libraries in a single run using Applied Biosystems' SOLiD sequencer. We applied our semi-automated pipeline to targeted medical resequencing of nuclear candidate genes in individuals affected by mitochondrial disorders. This novel method is capable of preparing as much as 32 DNA libraries in 2.01 days (8-hour workday for emulsion PCR/high throughput DNA sequencing, increasing sample preparation production by 8-fold.

  10. Maximum power analysis of photovoltaic module in Ramadi city

    Energy Technology Data Exchange (ETDEWEB)

    Shahatha Salim, Majid; Mohammed Najim, Jassim [College of Science, University of Anbar (Iraq); Mohammed Salih, Salih [Renewable Energy Research Center, University of Anbar (Iraq)

    2013-07-01

    Performance of photovoltaic (PV) module is greatly dependent on the solar irradiance, operating temperature, and shading. Solar irradiance can have a significant impact on power output of PV module and energy yield. In this paper, a maximum PV power which can be obtain in Ramadi city (100km west of Baghdad) is practically analyzed. The analysis is based on real irradiance values obtained as the first time by using Soly2 sun tracker device. Proper and adequate information on solar radiation and its components at a given location is very essential in the design of solar energy systems. The solar irradiance data in Ramadi city were analyzed based on the first three months of 2013. The solar irradiance data are measured on earth's surface in the campus area of Anbar University. Actual average data readings were taken from the data logger of sun tracker system, which sets to save the average readings for each two minutes and based on reading in each one second. The data are analyzed from January to the end of March-2013. Maximum daily readings and monthly average readings of solar irradiance have been analyzed to optimize the output of photovoltaic solar modules. The results show that the system sizing of PV can be reduced by 12.5% if a tracking system is used instead of fixed orientation of PV modules.

  11. Dyella thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate-oxidizing bacterium isolated from rhizosphere soil of sunflower (Helianthus annuus L.).

    Science.gov (United States)

    Anandham, Rangasamy; Kwon, Soon-Wo; Indira Gandhi, Pandiyan; Kim, Soo-Jin; Weon, Hang-Yeon; Kim, Yi-Seul; Sa, Tong-Min; Kim, Yong-Ki; Jee, Hyeong-Jin

    2011-02-01

    A Gram-negative, aerobic, motile, rod-shaped, thiosulfate-oxidizing bacterium, designated ATSB10(T), was isolated from rhizosphere soil of sunflower (Helianthus annuus L.). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ATSB10(T) was closely related to members of the genera Dyella (96.4-98.1 % 16S rRNA gene sequence similarity) and Luteibacter (96.4-97.0 %) and Fulvimonas soli LMG 19981(T) (96.7 %) and Frateuria aurantia IFO 3245(T) (97.8 %). The predominant fatty acids were iso-C(16 : 0), iso-C(17 : 1)ω9c and iso-C(15 : 0). The major quinone was Q-8. The G+C content of the genomic DNA was 66.0 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, an unknown phospholipid, unknown aminophospholipids and an unknown aminolipid. On the basis of phenotypic properties, phylogenetic distinctiveness and DNA-DNA relatedness, strain ATSB10(T) represents a novel species in the genus Dyella, for which the name Dyella thiooxydans sp. nov. is proposed. The type strain is ATSB10(T) (=KACC 12756(T) =LMG 24673(T)).

  12. Solar magnetic activity cycles, coronal potential field models and eruption rates

    CERN Document Server

    Petrie, G J D

    2013-01-01

    We study the evolution of the observed photospheric magnetic field and the modeled global coronal magnetic field during the past 3 1/2 solar activity cycles observed since the mid-1970s. We use synoptic magnetograms and extrapolated potential-field models based on longitudinal full-disk photospheric magnetograms from the NSO's three magnetographs at Kitt Peak, the Synoptic Optical Long-term Investigations of the Sun (SOLIS) vector spectro-magnetograph (VSM), the spectro-magnetograph and the 512-channel magnetograph instruments, and from the U. Stanford's Wilcox Solar Observatory. The associated multipole field components are used to study the dominant length scales and symmetries of the coronal field. Polar field changes are found to be well correlated with active fields over most of the period studied, except between 2003-6 when the active fields did not produce significant polar field changes. Of the axisymmetric multipoles, only the dipole and octupole follow the poles whereas the higher orders follow the ...

  13. Solution of the KdV Equation Based on COMSOL Multiphysics%基于COMSOL Multiphysics求解KdV方程

    Institute of Scientific and Technical Information of China (English)

    糜凯华

    2014-01-01

    In order to obtain numerical solutions of the KdV equation,COMSOL Multiphysics software based on finite element method established a finite element model and solved the distribution of solitary waves at various points in time. The results can study the propagation of solitary waves. Simulation results indicate that the soli-tary wave does not occur in isolation. Solitary waves can be found within a certain space where collisions occur as well as reproduced by representing as a three dimensional map distribution. Two different speed and ampli-tude solitons collided and did not affect each other.%为了得到KdV方程的数值解,基于有限单元法的COMSOL Multiphysics软件建立有限元模型并求解孤立波在各个时间点的分布情况,以研究孤立波的传播特性。数值模拟结果表明:孤立波不会发生消散,以三维图的分布形式表示,可以发现孤立子在一定的空间内会发生碰撞以及发生重现,也即两个不同速度和振幅的孤子相互碰撞、相互穿过,互相之间没有影响。

  14. Sunspot positions, areas, and group tilt angles for 1611-1631 from observations by Christoph Scheiner

    CERN Document Server

    Arlt, R; Schmiel, C; Spada, F

    2016-01-01

    Digital images of the observations printed in the books "Rosa Ursina sive solis" and "Prodromus pro sole mobili" by Christoph Scheiner as well as the drawings from Scheiner's letters to Marcus Welser are analysed in order to obtain information on positions and sizes of sunspots that appeared before the Maunder minimum. In most cases, the given orientation of the ecliptic is used to set up the heliographic coordinate system for the drawings. Positions and sizes are measured manually on the screen. Very early drawings have no indication of their orientation. A rotational matching using common spots of adjacent days is used in some cases, while in other cases, the assumption of images being aligned with a zenith-horizon coordinate system appeared to be the most probable. In total, 8167 sunspots were measured. A distribution of sunspot latitudes versus time (butterfly diagram) is obtained for Scheiner's observations. The observations of 1611 are very inaccurate, the drawings of 1612 have at least an indication of...

  15. Ultra deep sequencing of Listeria monocytogenes sRNA transcriptome revealed new antisense RNAs.

    Directory of Open Access Journals (Sweden)

    Sebastian Behrens

    Full Text Available Listeria monocytogenes, a gram-positive pathogen, and causative agent of listeriosis, has become a widely used model organism for intracellular infections. Recent studies have identified small non-coding RNAs (sRNAs as important factors for regulating gene expression and pathogenicity of L. monocytogenes. Increased speed and reduced costs of high throughput sequencing (HTS techniques have made RNA sequencing (RNA-Seq the state-of-the-art method to study bacterial transcriptomes. We created a large transcriptome dataset of L. monocytogenes containing a total of 21 million reads, using the SOLiD sequencing technology. The dataset contained cDNA sequences generated from L. monocytogenes RNA collected under intracellular and extracellular condition and additionally was size fractioned into three different size ranges from 150 nt. We report here, the identification of nine new sRNAs candidates of L. monocytogenes and a reevaluation of known sRNAs of L. monocytogenes EGD-e. Automatic comparison to known sRNAs revealed a high recovery rate of 55%, which was increased to 90% by manual revision of the data. Moreover, thorough classification of known sRNAs shed further light on their possible biological functions. Interestingly among the newly identified sRNA candidates are antisense RNAs (asRNAs associated to the housekeeping genes purA, fumC and pgi and potentially their regulation, emphasizing the significance of sRNAs for metabolic adaptation in L. monocytogenes.

  16. Ultra Deep Sequencing of Listeria monocytogenes sRNA Transcriptome Revealed New Antisense RNAs

    Science.gov (United States)

    Behrens, Sebastian; Widder, Stefanie; Mannala, Gopala Krishna; Qing, Xiaoxing; Madhugiri, Ramakanth; Kefer, Nathalie; Mraheil, Mobarak Abu; Rattei, Thomas; Hain, Torsten

    2014-01-01

    Listeria monocytogenes, a gram-positive pathogen, and causative agent of listeriosis, has become a widely used model organism for intracellular infections. Recent studies have identified small non-coding RNAs (sRNAs) as important factors for regulating gene expression and pathogenicity of L. monocytogenes. Increased speed and reduced costs of high throughput sequencing (HTS) techniques have made RNA sequencing (RNA-Seq) the state-of-the-art method to study bacterial transcriptomes. We created a large transcriptome dataset of L. monocytogenes containing a total of 21 million reads, using the SOLiD sequencing technology. The dataset contained cDNA sequences generated from L. monocytogenes RNA collected under intracellular and extracellular condition and additionally was size fractioned into three different size ranges from 150 nt. We report here, the identification of nine new sRNAs candidates of L. monocytogenes and a reevaluation of known sRNAs of L. monocytogenes EGD-e. Automatic comparison to known sRNAs revealed a high recovery rate of 55%, which was increased to 90% by manual revision of the data. Moreover, thorough classification of known sRNAs shed further light on their possible biological functions. Interestingly among the newly identified sRNA candidates are antisense RNAs (asRNAs) associated to the housekeeping genes purA, fumC and pgi and potentially their regulation, emphasizing the significance of sRNAs for metabolic adaptation in L. monocytogenes. PMID:24498259

  17. Forenzika elektronske pošte

    Directory of Open Access Journals (Sweden)

    Milorad S. Markagić

    2013-10-01

    podataka, pa ih je jednostavnije analizirati i generisati izveštaj. Analiza elektronske pošte zasnovane na internet serverima Za personalnu komunikaciju često  se koriste Web servisi za elektronsku poštu: Google, Yahoo, Hotmail, gmail.... Ovi se servisi koriste bez upotrebe softvera klijenata elektronske pošte i sačinjavaju klijent-server sistem. Web mail sistem predaje elektronsku poruku koristeći SMTP protokol i koristeći POP ili IMAP protokole. Web pošta se ne čuva se na lokalnom računaru, osim ako to korisnik izričito ne zahteva, već ista ostaje memorisana na serveru. Pristup nalogu elektronske pošte na serveru ili prikupljanje podataka o korisničkom nalogu od strane pružaoca usluga umnogome smanjuje količinu posla forenzičkom istražiocu. Ako pristup nije dozvoljen, do web mail poruka može se doći pregledom radne i keš memorije. Kada korisnik proverava poruke, ili sastavlja novu, operativni sistem sačuva podatke sa ekrana na hard disku, pogotovo ako korisniku treba više  vreme da sastavi poruku. Privremeni dokumenti Operativni sistemi privremene dokumente kreiraju za aplikacije koje šalju i primaju podatke preko mreže. Ti se podaci prvo skladište u radnoj memoriji, a kada se ista popuni, operativni sistem pomera podatke niže na listu prioriteta podataka koji su potrebni aplikacijama i zapisuje ih na tvrdi disk. Ne postoji posebno i jedinstveno područje gde se čuvaju privremeni dokumenti jer neke aplikacije stvaraju dodatne privremene datoteke uz one što ih stvara operativni sistem. Aplikacije koje nemaju mogućnost privremenog čuvanja podataka, prepuštaju operativnom sistemu da ih memoriše koristeći swap datoteke ili virtualnu memoriju. Kada aplikaciji zatreba privremeno sačuvani podatak, operativni sistem ga šalje aplikaciji i briše sa hard diska. Iako se swap datoteke brišu, do podataka je ipak moguće doći, jer još uvek fizički postoje na disku Instant poruke U većim organizacijama komunikacija sa korisnicima se

  18. Demethylase Inhibitor Fungicide Resistance in Pyrenophora teres f. sp. teres Associated with Target Site Modification and Inducible Overexpression of Cyp51.

    Science.gov (United States)

    Mair, Wesley J; Deng, Weiwei; Mullins, Jonathan G L; West, Samuel; Wang, Penghao; Besharat, Naghmeh; Ellwood, Simon R; Oliver, Richard P; Lopez-Ruiz, Francisco J

    2016-01-01

    Pyrenophora teres f. sp. teres is the cause of net form of net blotch (NFNB), an economically important foliar disease in barley (Hordeum vulgare). Net and spot forms of net blotch are widely controlled using site-specific systemic fungicides. Although resistance to succinate dehydrogenase inhibitors and quinone outside inhibitors has been addressed before in net blotches, mechanisms controlling demethylation inhibitor resistance have not yet been reported at the molecular level. Here we report the isolation of strains of NFNB in Australia since 2013 resistant to a range of demethylase inhibitor fungicides. Cyp51A:KO103-A1, an allele with the mutation F489L, corresponding to the archetype F495I in Aspergillus fumigatus, was only present in resistant strains and was correlated with resistance factors to various demethylase inhibitors ranging from 1.1 for epoxiconazole to 31.7 for prochloraz. Structural in silico modeling of the sensitive and resistant CYP51A proteins docked with different demethylase inhibitor fungicides showed how the interaction of F489L within the heme cavity produced a localized constriction of the region adjacent to the docking site that is predicted to result in lower binding affinities. Resistant strains also displayed enhanced induced expression of the two Cyp51A paralogs and of Cyp51B genes. While Cyp51B was found to be constitutively expressed in the absence of fungicide, Cyp51A was only detected at extremely low levels. Under fungicide induction, expression of Cyp51B, Cyp51A2, and Cyp51A1 was shown to be 1.6-, 3,- and 5.3-fold higher, respectively in the resistant isolate compared to the wild type. These increased levels of expression were not supported by changes in the promoters of any of the three genes. The implications of these findings on demethylase inhibitor activity will require current net blotch management strategies to be reconsidered in order to avoid the development of further resistance and preserve the lifespan of

  19. Podrumko

    Directory of Open Access Journals (Sweden)

    Manfred Kyber

    2016-12-01

    Full Text Available Između ponoći i jednog sata oživi sve ono za što glupi ljudi vjeruju da oživjeti ne može. Ali zaista, mnogobrojne stvari koje inače samo ukočeno i mirno leže, kao da ne mogu reći ni „dobar dan“, sve one u to vrijeme ožive. I ne brinu mnogo o tome vjeruju li glupi ljudi u to ili ne. Tako i u tom starom gradiću oživje sve kada sat sa zvonika crkve Presvete Djevice Marije s dvanaest muklih, teških udaraca otkuca ponoć. Kamenje na pločniku počelo je razgovarati s vlatima trave koja je među njim rasla i pitalo je koliko još misli ostati. Zabati i erkeri kuća u uskim zamršenim uličicama kimali su jedni drugima, a ulične svjetiljke žalile su se na vjetar; prehladile su se jer on tako bezobzirno mijenja smjer.Također oživje sve i u starom vinskom podrumu staroga gradića. Mnogobrojne bačve koje stajahu jedne pokraj drugih, velike i male, zijevnuše i protegnuše se i ispružiše se, a kad bi jedna drugu pritom gurnula, rekla bi: „Oh, tisuću puta oprostite!“ Jer bačve su vrlo pristojne i znaju se lijepo ponašati. Tada bi sve uspravno stale na svoje debele nožice – naime, bačve imaju nožice, ako glupi ljudi ne znaju ni to – i sve bi se klanjale jedne drugima i kimale si i pozdravljale se na sve stane.

  20. Dosezanje izvan male znanstvene zajednice: modeli društvenog utjecaja na suradnju preko nacionalnih i disciplinarnih granica kod znanstvenika iz triju polja društvenih znanosti

    Directory of Open Access Journals (Sweden)

    Srebrenka Letina

    2016-12-01

    Full Text Available Suradnja sa znanstvenicima iz drugih područja znanosti ili drugih zemalja važna je općenito za napredak znanosti, osobito za znanstvenike koji djeluju u malim i perifernim znanstvenim zajednicama. Cilj je ovog istraživanja bio ispitati postoje li mrežni (strukturni efekti na vjerojatnost da će pojedini znanstvenik ostvariti koautorstvo/suradnju izvan svoje nacionalne i disciplinarne zajednice. Na temelju podataka o koautorstvu na publikacijama indeksiranim u dvjema međunarodnim bazama (Web of Science i Scopus i jednoj nacionalnoj bazi (Nacionalna i sveučilišna knjižnica konstruirana je mreža koautorstva za tri područja društvenih znanosti u Hrvatskoj. Testirana su tri strukturna prediktora (aktivnost, zaraza i popularnost /Ego-2Star/, a korištenje autologističkih modela atributa aktera omogućilo je istovremeno kontroliranje šest atributa aktera. Rezultati su potvrdili hipotezu o postojanju pozitivnog učinka zaraze (vjerojatnost suradnje sa znanstvenicima izvan nacionalne i disciplinarne mreže veća je ako neposredni alter u mreži također ima vanjske suradnje i negativnog učinka aktivnosti (oni koji surađuju s jednim ili više znanstvenika izvan svoje mreže, manje surađuju unutar svoje mreže u mreži obrazovnih znanosti. Međutim, u mreži sociologa utvrđen je samo efekt negativne aktivnosti, dok u mreži psihologa nijedan strukturni efekt nije bio značajan prediktor suradnje izvan uže zajednice. Pronađen je značajan efekt broja radova napisanih u koautorstvu, ali ne i broja jednoautorskih radova, te dobi u svim trima poljima. Spol, lokacija i maksimalna snaga veze imali su različitu ulogu u trima istraživanim poljima. U radu su naglašeni metodološki aspekti analize mreža koautorstva i predlažu smjernice za buduća istraživanja.

  1. Assessment of the efficacy of first-line antimalarial drugs after 5 years of deployment by the National Malaria Control Programme in Côte d'Ivoire

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    Offianan AT

    2011-11-01

    Full Text Available Andre T Offianan1, Serge B Assi2, Aristide MA Coulibaly1, Landry T N'guessan1, Aristide A Ako1, Florence K Kadjo2, Moïse K San2, Louis K Penali2 1Malariology Department, Institut Pasteur de Côte d'Ivoire, Abidjan, Côte d'Ivoire; 2National Malaria Control Programme, Abidjan, Côte d'Ivoire Background: The emergence of artemisinin resistance has raised concerns that the most potent antimalarial drug may be under threat. Artesunate + amodiaquine (ASAQ and artemether-lumefantrine (AL are respectively the first- and second-line treatments for uncomplicated falciparum malaria in Côte d'Ivoire. A comparison of the efficacy and safety of these two drug combinations was necessary to make evidence-based drug treatment policies. Methods: In an open-label, non inferiority, randomized, controlled clinical trial, children aged 6–59 months were randomized to receive ASAQ or AL. Both drug regimens were given for 3 days, and follow-up was for 28 days. The primary endpoint was the 28-day cure rates and was defined as proportion of patients with polymerase chain reaction (PCR-corrected cure rate after 28 days of follow-up. Findings: A total of 251 patients who were attending the Ayame and Dabakala hospitals and presenting with symptomatic acute uncomplicated falciparum malaria were randomized to receive ASAQ (128 and AL (123. The intention-to-treat analysis showed effectiveness rates of 94.5% and 93.5% for ASAQ and AL, respectively on day 28. After adjustment for PCR results, these rates were 96.1% and 96.8%, respectively. On day 28, the per-protocol analysis showed effectiveness rates of 98.4% and 96.6% for ASAQ and AL, respectively. After adjustment by PCR for reinfection, these rates were 100% for each drug, and both regimens were well tolerated. Conclusion: ASAQ and AL remain efficacious treatments of uncomplicated falciparum malaria in Ivorian children 5 years after adoption. The efficacy of ASAQ and AL in Côte d'Ivoire requires, therefore, continuous

  2. Conservation measures undertaken to improve the population status of eastern imperial eagle (Aquila heliaca in Bulgaria

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    Demerdzhiev Dimitar

    2014-01-01

    Full Text Available Štúdia prezentuje výsledky ochranárskych opatrení a ich vplyv na populáciu orla královského v Bulharsku. Druh sa vyskytuje v juhovýchodnej casti krajiny, v centrálnom Bulharsku je známy len jeden pár. V období 2009-201 3 bolo obsadených 28 rôznych hniezdnych teritórií. Populácia narástla o 20 %, pocet párov s hniezdnymi teritóriami sa zvýšil z 20 v roku 2009 na 24 v roku 201 3. Stredné hodnoty parametrov hniezdenia orlov královských boli v období 2009-201 3 nasledovné: produktivita (P 1 ,03 ± 0,1 8; hniezdna úspešnost (BS 1 ,1 8±0,21 , úspešnost vyletenia mládat (FS 1 .68±0,1 6. Rocná miera prežitia hniezdiacich jedincov bola 0,795-0,932. Najdôležitejším faktorom, spôsobujúcim mortalitu mladých orlov boli usmrtenia po zásahu elektrickým prúdom spolocne s nelegálnymi odstrelmi a otravami. Strážené páry mali vyššiu hniezdnu úspešnost (1 ,32±0,88 ako nestrážené páry (1 ,06±0,91 . Celkom bolo obsadených vtákmi rôznych druhov 39 % umelých hniezd inštalovaných v období 2009-201 3. V období 2008-201 3 bolo celkom 23 mladých orlov královských oznacených satelitnými vysielackami s GPS jednotkou. V teritóriách siedmich párov bolo izolovaných 608 nebezpecných stlpov elektrického vedenia. V jesennom a zimnom období (október až marec sa raz za týžden realizovalo prikrmovanie 1 4 párov orlov. Celkom sa zrealizovalo 483 prikrmovaní, na 450 z nich bolo súcastou sledovanie efektivity tejto cinnosti.

  3. Distribution, abundance, breeding parameters, threats and prey preferences of the eastern imperial eagle (Aquila heliaca in European Turkey

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    Demerdzhiev Dimitar

    2014-01-01

    Full Text Available V období 2008-201 3 bolo orlami královskými obsadených 32 rôznych hniezdnych teritórií. Tieto územia sa nachádzajú najmä v dvoch oblastiach: pohorie Derventa/Yıldız a územie na sever od Marmarského mora. Najbližšia vzdialenost medzi hniezdiskami bola 4,2 km. Stredná vzdialenost medzi pármi bola 1 0,44 ± 2,95 km. Priemerná hodnota hniezdnej úspešnosti v období 2008-201 3 vrátane 1 07 prípadov inkubácii bola 1 ,01±0,1 0. Priemerná hniezdna úspešnost vtákov v Marmarskom regióne (1 ,05±0,78 bola vyššia ako v oblasti pohorí Derventa/Yıldız (0,91±0,84. Predkladaná štúdia potravy orla královského bola založená na 582 zvyškoch koristi, obsahujúcej minimálne 70 taxónov. Cicavce boli najrozšírenejšie skupinou (46.5 %, nasledované vtákmi (32,4 % a plazmi (20,3 %. Najcastejšou koristou bol jež bledý (Erinaceus roumanicus a dva druhy korytnaciek - korytnacka zelenkastá (Eurotestudo hermanni a korytnacka grécka (Testudo graeca, ktoré dohromady predstavovali celkom 1 4,4 % z ulovenej koristi. Potravné spektrum sledovaných párov obsahoval tieto druhy koristi: cajka žltonohá (Larus michahellis, sysel pasienkový (Spermophilus citellus, domáca sliepka (Gallus gallus f. domestica a bocian biely (Ciconia ciconia. Tieto druhy dohromady tvorili 67,8 % z ulovenej koristi. Bol preukázaný štatisticky významný rozdiel (Z=2,52, p=0,01 v preferencii potravy orlov královských v oboch študovaných oblastiach.

  4. Afferent Input Selects NMDA Receptor Subtype to Determine the Persistency of Hippocampal LTP in Freely Behaving Mice

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    Ballesteros, Jesús J.; Buschler, Arne; Köhr, Georg; Manahan-Vaughan, Denise

    2016-01-01

    The glutamatergic N-methyl-D-aspartate receptor (NMDAR) is critically involved in many forms of hippocampus-dependent memory that may be enabled by synaptic plasticity. Behavioral studies with NMDAR antagonists and NMDAR subunit (GluN2) mutants revealed distinct contributions from GluN2A- and GluN2B-containing NMDARs to rapidly and slowly acquired memory performance. Furthermore, studies of synaptic plasticity, in genetically modified mice in vitro, suggest that GluN2A and GluN2B may contribute in different ways to the induction and longevity of synaptic plasticity. In contrast to the hippocampal slice preparation, in behaving mice, the afferent frequencies that induce synaptic plasticity are very restricted and specific. In fact, it is the stimulus pattern and not variations in afferent frequency that determine the longevity of long-term potentiation (LTP) in vivo. Here, we explored the contribution of GluN2A and GluN2B to LTP of differing magnitudes and persistence in freely behaving mice. We applied differing high-frequency stimulation (HFS) patterns at 100 Hz to the hippocampal CA1 region, to induce NMDAR-dependent LTP in wild-type (WT) mice, that endured for 24 h (late (L)-LTP). In GluN2A-knockout (KO) mice, E-LTP (HFS, 50 pulses) was significantly reduced in magnitude and duration, whereas LTP (HFS, 2 × 50 pulses) and L-LTP (HFS, 4 × 50 pulses) were unaffected compared to responses in WT animals. By contrast, pharmacological antagonism of GluN2B in WT had no effect on E-LTP but significantly prevented LTP. E-LTP and LTP were significantly impaired by GluN2B antagonism in GluN2A-KO mice. These data indicate that the pattern of afferent stimulation is decisive for the recruitment of distinct GluN2A and GluN2B signaling pathways that in turn determine the persistency of hippocampal LTP. Whereas brief bursts of patterned stimulation preferentially recruit GluN2A and lead to weak and short-lived forms of LTP, prolonged, more intense, afferent activation recruits

  5. Modelling the Uncertainty of Slope Estimation from a Lidar-Derived Dem: a Case Study from a Large-Scale Area in the Czech Republic / Modelovanie Neistoty Vo Výpočte Sklonov Z Lidarových Dmr; Prípadová Štúdia Vybraného Malého Územia V Čr

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    Mudron Ivan

    2013-06-01

    Full Text Available Táto práca zhŕňa metódu a výsledky modelovania chýb a analýzu šírenia chýb vo výpočte sklonov z DMR získaných LIDAR-om v skúmanej lokalite okolia sútoku riek Olše a Stonávka. V terénnych analýzach výstupy uvedenej analýzy sú vždy funkciou vstupu. Na generovania pola výškových chýb boli použité dve rozdielne metódy podľa vstupných dát. Modelované chyby v nadmorských výškach následne vstupovali do analýzy šírenia chýb. Hlavným cieľom práce bolo tak ako aj poukázanie na význam kvality vstupných dát vo výpočte sklonov a odhad šírenej chyby z nadmorských výšok v sklonoch tak aj identifikácia chýb v DMR a ich dopad. Závislosti chýb boli vyhodnotené hlavne pre lepší odhad chyby v sklonoch. V simuláciách boli použité 4 vstupné DMR s rozlíšením 0.5, 1, 5 a 10 metrov s RMSE chybou do 0.317 metra (10 m DMR. Všetky DMR boli získané z mračna bodov získaných LIDAR metódou zberu dát. Šírenie chýb bolo modelované pomocou stochastickej simulácie Monte Carlo s 250 iteráciami. Článok sa zameriava na šírenie chýb z vysoko presných vstupných dát na malom území. RMSE chyba bola získaná v prvom prípade z dát získaných terénnym prieskumom (RTK GPS a v druhom prípade z porovnania dvoch kvalitatívne rozdielnych DMR. V prvom prípade sa vypočítali chyby vo výškach pomocou náhodného generátora chýb bez autokorelácie chýb. V druhom prípade sa s pomocou semivariogramu namodelovalo autokorelované pole chýb vo výškach. Použitím vhodných štatistík boli odvodené výsledky simulácie a overené stanovené hypotézy. Tak ako sa očakávalo chyby v sklonoch sú vyššie s zvyšujúcou sa chybou v nadmorských výškach. Tiež závislosti chýb od vypočítaných sklonov boli preskúmané, kde sa potvrdila závislosť chýb na sklonoch. Na druhej strane geológia nemala žiaden vplyv na chybu v sklonoch. Chyby namodelované bez autokorelácie nevedú vo v

  6. Des tables pascales aux tables astronomiques et retour.

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    Max Lejbowicz

    2006-05-01

    Full Text Available L’article étudie la naissance et le développement du calendrier ecclésiastique chrétien, i. e. le comput, depuis les premiers témoignages de la célébration annuelle de la résurrection de Jésus jusqu’aux traductions des tables astronomiques arabes au xiie siècle. Il privilégie les procédures qui aboutissent à la détermination des dates pascales et à leur mise en forme tabulaire. Les analyses sont conduites à partir d’un double point de vue. L’un est scientifique. Il s’appuie sur les données astronomiques retenues par Ptolémée et sur l’apport de la tradition mathématique grecque au calcul par approximations. Les cycles soli-lunaires sont posés à partir des fractions continues et le cycle soli-hebdomadaire à partir du plus petit commun multiple. Le second point de vue est social : l’unification du comput participe à celle de la chrétienté comprise comme une configuration politico-religieuse. Deux conclusions s’imposent. Quelle que soit l’importance que la civilisation médiévale a attribuée au comput, il reste que : 1 / les Pâques sont porteuses de significations irréductibles aux techniques chronométriques qui inscrivent cette fête dans le déroulement de l’année ; 2 / ces techniques ont toutefois marqué profondément les curiosités intellectuelles des Latins et les ont préparé à accueillir avec ferveur les zīj et la numération de position.The article studies the birth and development of the Christian ecclesiastical calendar, i.e. the computus, from the first witnesses to the yearly celebration of the resurrection of Jesus to the translations of arabic astronomical tables in the 12th century. It focuses on the procedures which resulted in determinig the dates of Easter and their being put into tabular form. These analyses were undertaken from two perspectives. One was scientific, relying on the astronomical data preserved by Ptolemy and on the contribution of the Greek mathematical

  7. Annotation-based genome-wide SNP discovery in the large and complex Aegilops tauschii genome using next-generation sequencing without a reference genome sequence

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    Luo Ming-Cheng

    2011-01-01

    Full Text Available Abstract Background Many plants have large and complex genomes with an abundance of repeated sequences. Many plants are also polyploid. Both of these attributes typify the genome architecture in the tribe Triticeae, whose members include economically important wheat, rye and barley. Large genome sizes, an abundance of repeated sequences, and polyploidy present challenges to genome-wide SNP discovery using next-generation sequencing (NGS of total genomic DNA by making alignment and clustering of short reads generated by the NGS platforms difficult, particularly in the absence of a reference genome sequence. Results An annotation-based, genome-wide SNP discovery pipeline is reported using NGS data for large and complex genomes without a reference genome sequence. Roche 454 shotgun reads with low genome coverage of one genotype are annotated in order to distinguish single-copy sequences and repeat junctions from repetitive sequences and sequences shared by paralogous genes. Multiple genome equivalents of shotgun reads of another genotype generated with SOLiD or Solexa are then mapped to the annotated Roche 454 reads to identify putative SNPs. A pipeline program package, AGSNP, was developed and used for genome-wide SNP discovery in Aegilops tauschii-the diploid source of the wheat D genome, and with a genome size of 4.02 Gb, of which 90% is repetitive sequences. Genomic DNA of Ae. tauschii accession AL8/78 was sequenced with the Roche 454 NGS platform. Genomic DNA and cDNA of Ae. tauschii accession AS75 was sequenced primarily with SOLiD, although some Solexa and Roche 454 genomic sequences were also generated. A total of 195,631 putative SNPs were discovered in gene sequences, 155,580 putative SNPs were discovered in uncharacterized single-copy regions, and another 145,907 putative SNPs were discovered in repeat junctions. These SNPs were dispersed across the entire Ae. tauschii genome. To assess the false positive SNP discovery rate, DNA

  8. Genome sequencing of methanogenic Archaea Methanosarcina mazei TUC01 strain isolated from an Amazonian Flooded Area

    Science.gov (United States)

    Baraúna, R. A.; Graças, D. A.; Ramos, R. T.; Carneiro, A. R.; Lopes, T. S.; Lima, A. R.; Zahlouth, R. L.; Pellizari, V. H.; Silva, A.

    2013-05-01

    Methanosarcina mazei is a strictly anaerobic methanogen from the Methanosarcinales order. This species is known for its broad catabolic range among methanogens and is widespread throughout diverse environments. The draft genome of a strain cultivated from the sediment of the Tucuruí hydroelectric power station, the fourth largest hydroelectric dam in the world, is described here. Approximately 80% of methane is produced by biogenic sources, such as methanogenic archaea from M. mazei species. Although the methanogenesis pathway is well known, some aspects of the core genome, genome evolution and shared genes are still unclear. A sediment sample from the Tucuruí hydropower station reservoir was inoculated in mineral media supplemented with acetate and methanol. This media was maintained in an H2:CO2 (80:20) atmosphere to enrich and cultivate M. mazei. The enrichment was conducted at 30°C under standard anaerobic conditions. After several molecular and cellular analyses, total DNA was extracted from a non-pure culture of M. mazei, amplified using phi29 DNA polymerase (BioLabs) and finally used as a source template for genome sequencing. The draft genome was obtained after two rounds of sequencing. First, the genome was sequenced using a SOLiD System V3 with a mate-paired library, which yielded 24,405,103 and 24,399,268 reads (50 bp) for the R3 and F3 tags, respectively. The second round of sequencing was performed using the SOLiD 5500 XL platform with a mate-paired library, resulting in a total of 113,588,848 reads (60 bp) for each tag (F3 and R3). All reads obtained by this procedure were filtered using Quality Assessment software, whereby reads with an average quality score below Phred 20 were removed. Velvet and Edena were used to assemble the reads, and Simplifier was used to remove the redundant sequences. After this, a total of 16,811 contigs were obtained. M. mazei GO1 (AE008384) genome was used to map the contigs and generate the scaffolds. We used the

  9. Deformations of the Protection Shelf in the "Wapno" Salt Mine, Based on Model Studies

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    Kortas, Grzegorz; Maj, Agnieszka

    2014-12-01

    The catastrophic mine failure resulting from the inrush of water into the Wapno Salt Mine was probably caused by a fracture in the roof protection shelf. The purpose of the present study was to apply the method of the homogenization of the multi-level room-and-pillar structure in the Wapno Salt Mine for 3D+t modelling of the elastic-viscous medium to determine the distribution of stress and strain, and, on that basis, to estimate the geomechanical conditions existing in the roof shelf. This paper presents briefly the spatial development of the salt mine's structure and the results of the surveying measurements carried out during the salt mine's operation and after the mine was flooded. Those results constituted a basis for the verification of the introduced homogenization parameters, i.e. the time-dependent changes of the elasticity modulus and the susceptibility to creep at particular salt mine's levels. A simulated process demonstrated the development of positive values of principle stresses, increasing with time, and of omnidirectional tensile strains. In such conditions, the cracking of the protection shelf body could proceed, also with opening of water flow paths from the dome cap into the salt mine's workings. This paper presents a new research method, the results of its application, and the obtained distribution of stresses and strains that can be useful for the assessment of water hazard in other salt mines. Katastrofa górnicza wywołana wdarciem wody do kopalni soli w Wapnie spowodowana była prawdopodobnie pęknięciem ochronnej półki stropowej. Celem tej pracy było zastosowanie metody homogenizacji struktury komorowo-filarowej wielopoziomowej kopalni Wapno do modelowania ośrodka sprężysto-lepkiego 3D+t dla określenia rozkładu naprężeń i odkształceń oraz na tej podstawie oszacowania warunków geomechanicznych w półce stropowej. W pracy przedstawiono krótko rozwój przestrzenny struktury kopalni oraz wyniki przeprowadzonych pomiar

  10. Mobilisation against International Human Rights: Re-domesticating the Dominican Citizenship Regime

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    Leiv Marsteintredet

    2016-04-01

    Full Text Available El artículo analiza la movilización doméstica en contra de la implementación de los derechos humanos internacionales en el ámbito del régimen de la nacionalidad en la República Dominicana. Mientras que los promotores de derechos humanos ganaron todas las batallas legales en el Sistema Interamericano de Derechos Humanos, los grupos que movilizaron en contra de los derechos humanos en la República Dominicana movilizaron más eficientemente a nivel doméstico, y ganaron la guerra en las instituciones dominicanas. El artículo demuestra que la movilización en contra de los derechos humanos fue una respuesta consciente a la Corte Interamericana de Derechos Humanos y el caso de 'Yean 'y 'Bosico'. La movilización unió a todos los actores políticos dominantes en el Congreso y en las cortes en acciones legales y políticas para redefinir y re-domesticar el control sobre el régimen de nacionalidad dominicano en base de una definición más restrictiva del 'ius soli. 'Esta movilización culminó con la salida de la República Dominicana de la Corte Interamericana de Derechos Humanos en 2014. El caso dominicano demuestra que una reacción negativa ("backlash" a derechos humanos puede demostrarse inicialmente de maneras muy sutiles y que para mejorar el cumplimiento a derechos humanos internacionales es imprescindible movilizar no sólo a nivel internacional sino también dentro de las instituciones domésticas. English: This article traces and analyses the domestic mobilisation against the implementation of international human rights with respect to the citizenship regime in the Dominican Republic. Whereas the human rights promoters won every legal battle on the right to citizenship in the Inter-American System of Human Rights, groups mobilising against human rights, the pro-violation constituency, were more effective domestically and won the war in all domestic institutions. As a response to the Inter-American Court of Human Rights and the

  11. Ago2 immunoprecipitation identifies predicted microRNAs in human embryonic stem cells and neural precursors.

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    Loyal A Goff

    Full Text Available BACKGROUND: MicroRNAs are required for maintenance of pluripotency as well as differentiation, but since more microRNAs have been computationally predicted in genome than have been found, there are likely to be undiscovered microRNAs expressed early in stem cell differentiation. METHODOLOGY/PRINCIPAL FINDINGS: SOLiD ultra-deep sequencing identified >10(7 unique small RNAs from human embryonic stem cells (hESC and neural-restricted precursors that were fit to a model of microRNA biogenesis to computationally predict 818 new microRNA genes. These predicted genomic loci are associated with chromatin patterns of modified histones that are predictive of regulated gene expression. 146 of the predicted microRNAs were enriched in Ago2-containing complexes along with 609 known microRNAs, demonstrating association with a functional RISC complex. This Ago2 IP-selected subset was consistently expressed in four independent hESC lines and exhibited complex patterns of regulation over development similar to previously-known microRNAs, including pluripotency-specific expression in both hESC and iPS cells. More than 30% of the Ago2 IP-enriched predicted microRNAs are new members of existing families since they share seed sequences with known microRNAs. CONCLUSIONS/SIGNIFICANCE: Extending the classic definition of microRNAs, this large number of new microRNA genes, the majority of which are less conserved than their canonical counterparts, likely represent evolutionarily recent regulators of early differentiation. The enrichment in Ago2 containing complexes, the presence of chromatin marks indicative of regulated gene expression, and differential expression over development all support the identification of 146 new microRNAs active during early hESC differentiation.

  12. Characterization of the Burkholderia thailandensis SOS response by using whole-transcriptome shotgun sequencing.

    Science.gov (United States)

    Ulrich, Ricky L; Deshazer, David; Kenny, Tara A; Ulrich, Melanie P; Moravusova, Anna; Opperman, Timothy; Bavari, Sina; Bowlin, Terry L; Moir, Donald T; Panchal, Rekha G

    2013-10-01

    The bacterial SOS response is a well-characterized regulatory network encoded by most prokaryotic bacterial species and is involved in DNA repair. In addition to nucleic acid repair, the SOS response is involved in pathogenicity, stress-induced mutagenesis, and the emergence and dissemination of antibiotic resistance. Using high-throughput sequencing technology (SOLiD RNA-Seq), we analyzed the Burkholderia thailandensis global SOS response to the fluoroquinolone antibiotic, ciprofloxacin (CIP), and the DNA-damaging chemical, mitomycin C (MMC). We demonstrate that a B. thailandensis recA mutant (RU0643) is ∼4-fold more sensitive to CIP in contrast to the parental strain B. thailandensis DW503. Our RNA-Seq results show that CIP and MMC treatment (P SOS response were induced and include lexA, uvrA, dnaE, dinB, recX, and recA. At the genome-wide level, we found an overall decrease in gene expression, especially for genes involved in amino acid and carbohydrate transport and metabolism, following both CIP and MMC exposure. Interestingly, we observed the upregulation of several genes involved in bacterial motility and enhanced transcription of a B. thailandensis genomic island encoding a Siphoviridae bacteriophage designated E264. Using B. thailandensis plaque assays and PCR with B. mallei ATCC 23344 as the host, we demonstrate that CIP and MMC exposure in B. thailandensis DW503 induces the transcription and translation of viable bacteriophage in a RecA-dependent manner. This is the first report of the SOS response in Burkholderia spp. to DNA-damaging agents. We have identified both common and unique adaptive responses of B. thailandensis to chemical stress and DNA damage.

  13. Mucilaginibacter carri sp. nov., isolated from a car air conditioning system.

    Science.gov (United States)

    Kim, Dong-Uk; Lee, Hyosun; Kim, Hyun; Kim, Song-Gun; Park, So Yoon; Ka, Jong-Ok

    2016-04-01

    A Gram-stain-negative, pink bacterial strain, designated PR0008KT, was isolated from an automobile evaporator core in Korea. The cells were obligately aerobic and rod-shaped. The strain grew at 10-40 °C (optimum, 20 °C), at pH 5-8 (optimum, 7), and in the presence of 0-1.5% (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus Mucilaginibacter (93.4-97.0% 16S rRNA sequence similarities) and showed a high sequence similarity with Mucilaginibacter litoreus BR-18T, Mucilaginibacter lutimaris BR-3T and Mucilaginibacter soli R9-65T (97.0%, 96.9% and 96.9% 16S rRNA sequence similarity, respectively). It contained summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16 : 0, iso-C17:0 3-OH and C16:0 as the predominant fatty acids and MK-7 as the major menaquinone. The polar lipids were phosphatidylethanolamine, one unknown aminophospholipid, two unknown aminolipids and two unknown polar lipids. The DNA G+C content of this strain was 47.4 mol%. Based on the phenotypic, genotypic and chemotaxonomic data, strain PR0008KT represents a novel species in the genus Mucilaginibacter, for which the name Mucilaginibacter carri sp. nov. (=KACC 17938T=NBRC 111539T) is proposed.

  14. Bacillus oryzisoli sp. nov., isolated from rice rhizosphere.

    Science.gov (United States)

    Zhang, Xiao-Xia; Gao, Ju-Sheng; Zhang, Lei; Zhang, Cai-Wen; Ma, Xiao-Tong; Zhang, Jun

    2016-09-01

    The taxonomy of strain 1DS3-10T, a Gram-staining-positive, endospore-forming bacterium isolated from rice rhizosphere, was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the novel strain was grouped with established members of the genus Bacillus and appeared to be closely related to the type strains Bacillus benzoevorans DSM 5391T (97.9 %), Bacillus circulans DSM 11T (97.7 %), Bacillus novalis JCM 21709T (97.3 %), Bacillus soli JCM 21710T (97.3 %), Bacillus oceanisediminis CGMCC 1.10115T (97.3 %) and BacillusnealsoniiFO-92T (97.1 %). The fatty acid profile of strain 1DS3-10T, which showed a predominance of iso-C15 : 0 and anteiso-C15 : 0, supported the allocation of the strain to the genus Bacillus. The predominant menaquinone was MK-7 (100 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminolipids. Cell-wall peptidoglycan contained meso-diaminopimelic acid. DNA-DNA hybridization values between strain 1DS3-10T and the type strains of closely related species were 25-33 %, which supported that 1DS3-10T represented a novel species in the genus Bacillus. The results of some physiological and biochemical tests also allowed the phenotypic differentiation of strain 1DS3-10T from the most closely related recognized species. On the basis of the phylogenetic and phenotypic evidence, strain 1DS3-10T represents a novel species of the genus Bacillus, for which the name Bacillus oryzisoli sp. nov. is proposed. The type strain of the novel species is 1DS3-10T (=ACCC 19781T=DSM 29761T).

  15. Alteration of the exDNA profile in blood serum of LLC-bearing mice under the decrease of tumour invasion potential by bovine pancreatic DNase I treatment

    Science.gov (United States)

    Brenner, Evgenyi V.; Kurilshikov, Alexander M.; Patutina, Olga A.; Zenkova, Marina A.

    2017-01-01

    Taking into account recently obtained data indicating the participation of circulating extracellular DNA (exDNA) in tumorigenesis, enzymes with deoxyribonucleic activity have again been considered as potential antitumour and antimetastatic drugs. Previously, using murine Lewis lung carcinoma and hepatocellular carcinoma A1 tumour models, we have shown the antimetastatic activity of bovine DNase I, which correlates with an increase of DNase activity and a decrease of exDNA concentration in the blood serum of tumour-bearing mice. In this work, using next-generation sequencing on the ABS SOLiD™ 5.500 platform, we performed a search for molecular targets of DNase I by comparing the exDNA profiles of healthy animals, untreated animals with Lewis lung carcinoma (LLC) and those with LLC treated with DNase I. We found that upon DNase I treatment of LLC-bearing mice, together with inhibition of metastasis, a number of strong alterations in the patterns of exDNA were observed. The major differences in exDNA profiles between groups were: i) the level of GC-poor sequences increased during tumour development was reduced to that of healthy mice; ii) levels of sequences corresponding to tumour-associated genes Hmga2, Myc and Jun were reduced in the DNase I-treated group in comparison with non-treated mice; iii) 224 types of tandem repeat over-presented in untreated LLC-bearing mice were significantly reduced after DNase I treatment. The most important result obtained in the work is that DNase I decreased the level of B-subfamily repeats having homology to human ALU repeats, known as markers of carcinogenesis, to the level of healthy animals. Thus, the obtained data lead us to suppose that circulating exDNA plays a role in tumour dissemination, and alteration of multiple molecular targets in the bloodstream by DNase I reduces the invasive potential of tumours. PMID:28222152

  16. Acinetobacter indicus sp. nov., isolated from a hexachlorocyclohexane dump site.

    Science.gov (United States)

    Malhotra, Jaya; Anand, Shailly; Jindal, Swati; Rajagopal, Raman; Lal, Rup

    2012-12-01

    The taxonomic position of a Gram-negative, non-motile, oxidase negative and catalase positive strain, A648(T), isolated from a hexachlorocyclohexane (HCH) dump site located in Lucknow, India, was ascertained by using a polyphasic approach. A comparative analysis of a partial sequence of the rpoB gene and the 16S rRNA gene sequence revealed that strain A648(T) belonged to the genus Acinetobacter. DNA-DNA relatedness values between strain A648(T) and other closely related members (16S rRNA gene sequence similarity greater than 97%), namely Acinetobacter radioresistens DSM 6976(T), A. venetianus ATCC 31012(T), A. baumannii LMG 1041(T), A. parvus LMG 21765(T) A. junii LMG 998(T) and A. soli JCM 15062(T), were found to be less than 8%. The major cellular fatty acids of strain A648(T) were 18:1ω9c (19.6%), summed feature 3 (15.9%), 16:0 (10.6%) and 12:0 (6.4%). The DNA G+C content was 40.4 mol%. The polar lipid profile of strain A648(T) indicated the presence of diphosphatidylglycerol, phosphatidylethanolamine, followed by phosphatidylglycerol and phosphatidylcholine. The predominant polyamine of strain A648(T) was 1,3-diaminopropane and moderate amounts of putrescine, spermidine and spermine were also detected. The respiratory quinone consisted of ubiquinone with nine isoprene units (Q-9). On the basis of DNA-DNA hybridization, phenotypic characteristics and chemotaxonomic and phylogenetic comparisons with other members of the genus Acinetobacter, strain A648(T) is found to be a novel species of the genus Acinetobacter, for which the name Acinetobacter indicus sp. nov. is proposed. The type strain is A648(T) ( = DSM 25388(T) = CCM 7832(T)).

  17. Scale evolution, sequence phylogeny, and taxonomy of thaumatomonad Cercozoa: 11 new species and new genera Scutellomonas, Cowlomonas, Thaumatospina and Ovaloplaca.

    Science.gov (United States)

    Scoble, Josephine M; Cavalier-Smith, Thomas

    2014-06-01

    We describe 11 new species of Thaumatomonadida using light and electron microscopy and rDNA gene sequences (18S, ITS1, 5.8S, ITS2). We found clear distinctions between major clades in molecular and morphological traits that support now splitting Thaumatomastix into three genera: new marine genera Ovaloplaca (oval plate-scales) and Thaumatospina (triangular plate-scales), both with distinctive radially-symmetric bobbin-based spine-scales, restricting Thaumatomastix to freshwater species with putatively non-homologous eccentric-spine scales and thicker triangular plate-scales. New genus Scutellomonas lacks spine-scales, having oval plate-scales with deeply-dished upper tier as in Ovaloplaca, with which it forms a clade having short/absent anterior cilium. Cowlomonas gen. n. is possibly naked. We describe two new Allas species, two new Thaumatomonas, and one new Reckertia species, and transfer R. hindoni to Thaumatomonas. Triangular-scaled Reckertia has varied plate-scales and ciliary scales. Thaumatomonas rDNA trees reveal two clades: zhukovi/seravini (predominantly triangular scales); coloniensis/oxoniensis/lauterborni/constricta/solis (scales mostly oval). We hypothesise that the ancestor of Thaumatomonadidae had radially-symmetric bobbin-based spine-scales and triangular plate-scales, bobbin-based spine-scales being lost in one lineage and eccentric-spine scales evolving in Thaumatomastix. Bobbin-based spine-scales arguably evolved from triangular plate-scales and single-tier ciliary scales (Ovaloplaca and Reckertia only) from plate-scale rudiments. We present a unified scheme for scale evolution and development in Imbricatea.

  18. Biomechanical stability of a bioabsorbable self-retaining polylactic acid/nano-sized β-tricalcium phosphate cervical spine interbody fusion device in single-level anterior cervical discectomy and fusion sheep models

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    Cao L

    2012-11-01

    Full Text Available Lu Cao,1 Ping-Guo Duan,1 Xi-Lei Li,1 Feng-Lai Yuan,3 Ming-Dong Zhao,2 Wu Che,1 Hui-Ren Wang,1 Jian Dong11Department of Orthopedic Surgery, Zhongshan Hospital, State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China; 2Department of Orthopedic Surgery, Jinshan Hospital, Fudan University, Shanghai, China; 3Affiliated Third Hospital of Nantong University, Wuxi, ChinaPurpose: The aim of this study was to investigate the biomechanical stability provided by a novel, polylactic acid/nano-sized, β-tricalcium phosphate, bioabsorbable, self-retaining cervical fusion cage (BCFC.Methods: Quasistatic nonconstraining torques (maximum 1.5 NM induced flexion, extension, lateral bending (±1.5 NM, and axial rotation (±1.5 NM on 32 sheep cervical spines (C2–C5. The motion segment C3–C4 was first tested intact; the following groups were tested after complete discectomy: autologous tricortical iliac crest bone graft, Medtronic–Wego polyetheretherketone (PEEK cage, Solis PEEK cage, and BCFC. The autologous bone graft group was tested with an anterior plate. The mean range of motion (ROM was calculated from the load-displacement curves.Results: BCFC significantly decreased ROM in lateral bending and axial rotation compared to other implants, and no significant difference in ROM between two types of PEEK cages and BCFC could be observed in flexion and extension. Anterior cervical plate (ACP significantly decreased ROM in flexion and extension, but no significant difference in ROM between BCFC and bone graft plus ACP could be determined in lateral bending and axial rotation.Conclusion: The BCFC device showed better stability to autologous tricortical iliac crest bone graft and PEEK cages in single-level anterior cervical discectomy and fusion models and thus may be a potential alternative to the current PEEK cages.Keywords: biomechanics, cervical spine, cages, bioabsorbable, sheep

  19. The complete genomic sequence of pepper yellow leaf curl virus (PYLCV and its implications for our understanding of evolution dynamics in the genus polerovirus.

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    Aviv Dombrovsky

    Full Text Available We determined the complete sequence and organization of the genome of a putative member of the genus Polerovirus tentatively named Pepper yellow leaf curl virus (PYLCV. PYLCV has a wider host range than Tobacco vein-distorting virus (TVDV and has a close serological relationship with Cucurbit aphid-borne yellows virus (CABYV (both poleroviruses. The extracted viral RNA was subjected to SOLiD next-generation sequence analysis and used as a template for reverse transcription synthesis, which was followed by PCR amplification. The ssRNA genome of PYLCV includes 6,028 nucleotides encoding six open reading frames (ORFs, which is typical of the genus Polerovirus. Comparisons of the deduced amino acid sequences of the PYLCV ORFs 2-4 and ORF5, indicate that there are high levels of similarity between these sequences to ORFs 2-4 of TVDV (84-93% and to ORF5 of CABYV (87%. Both PYLCV and Pepper vein yellowing virus (PeVYV contain sequences that point to a common ancestral polerovirus. The recombination breakpoint which is located at CABYV ORF3, which encodes the viral coat protein (CP, may explain the CABYV-like sequences found in the genomes of the pepper infecting viruses PYLCV and PeVYV. Two additional regions unique to PYLCV (PY1 and PY2 were identified between nucleotides 4,962 and 5,061 (ORF 5 and between positions 5,866 and 6,028 in the 3' NCR. Sequence analysis of the pepper-infecting PeVYV revealed three unique regions (Pe1-Pe3 with no similarity to other members of the genus Polerovirus. Genomic analyses of PYLCV and PeVYV suggest that the speciation of these viruses occurred through putative recombination event(s between poleroviruses co-infecting a common host(s, resulting in the emergence of PYLCV, a novel pathogen with a wider host range.

  20. A comprehensive survey of miRNA repertoire and 3' addition events in the placentas of patients with pre-eclampsia from high-throughput sequencing.

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    Li Guo

    Full Text Available BACKGROUND: To gain insight into potential roles of isomiR spectrum and isomiRs with 3' additions in pre-eclampsia, we performed a comprehensive survey of miRNA repertoire and 3' addition events from placental samples with different degrees of pre-eclampsia by applying SOLiD sequencing platform. PRINCIPAL FINDINGS: Over 30% isomiRs were detected with 3' non-template additional nucleotides, especially for additional nucleotide of adenosine. However, these modified isomiRs showed a lower percentage of total miRNA expression (<15%. Generally, 1-3 abundant isomiRs from a given miRNA locus were identified, but none of them was detected with 3' additions. Different miRNAs indicated various isomiR spectrums and expression patterns. The most abundant isomiR spectrum, isomiR profile and expression pattern always were stability, but herein we found several exceptions across samples, especially between normal and diseased samples. At isomiR level, we detected a distinct subset of differentially expressed modified isomiRs between normal and diseased samples or between mild and severe samples. Gene Ontology analysis of their experimentally validated target genes revealed enrichment for specific biological process categories. CONCLUSIONS: The phenomenon of multiple isomiRs, especially for isomiRs with 3' additions, is not a random event during pre-miRNA processing. Varieties of isomiRs and expression patterns reveal potential functional implication and should be taken into account. The study enriches association of miRNAs and human disease, including potential roles of various miRNA variants and 3' addition events.

  1. Identification and validation of human papillomavirus encoded microRNAs.

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    Kui Qian

    Full Text Available We report here identification and validation of the first papillomavirus encoded microRNAs expressed in human cervical lesions and cell lines. We established small RNA libraries from ten human papillomavirus associated cervical lesions including cancer and two human papillomavirus harboring cell lines. These libraries were sequenced using SOLiD 4 technology. We used the sequencing data to predict putative viral microRNAs and discovered nine putative papillomavirus encoded microRNAs. Validation was performed for five candidates, four of which were successfully validated by qPCR from cervical tissue samples and cell lines: two were encoded by HPV 16, one by HPV 38 and one by HPV 68. The expression of HPV 16 microRNAs was further confirmed by in situ hybridization, and colocalization with p16INK4A was established. Prediction of cellular target genes of HPV 16 encoded microRNAs suggests that they may play a role in cell cycle, immune functions, cell adhesion and migration, development, and cancer. Two putative viral target sites for the two validated HPV 16 miRNAs were mapped to the E5 gene, one in the E1 gene, two in the L1 gene and one in the LCR region. This is the first report to show that papillomaviruses encode their own microRNA species. Importantly, microRNAs were found in libraries established from human cervical disease and carcinoma cell lines, and their expression was confirmed in additional tissue samples. To our knowledge, this is also the first paper to use in situ hybridization to show the expression of a viral microRNA in human tissue.

  2. Novel Conserved Genotypes Correspond to Antibiotic Resistance Phenotypes of E. coli Clinical Isolates.

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    Michelle C Swick

    Full Text Available Current efforts to understand antibiotic resistance on the whole genome scale tend to focus on known genes even as high throughput sequencing strategies uncover novel mechanisms. To identify genomic variations associated with antibiotic resistance, we employed a modified genome-wide association study; we sequenced genomic DNA from pools of E. coli clinical isolates with similar antibiotic resistance phenotypes using SOLiD technology to uncover single nucleotide polymorphisms (SNPs unanimously conserved in each pool. The multidrug-resistant pools were genotypically similar to SMS-3-5, a previously sequenced multidrug-resistant isolate from a polluted environment. The similarity was evenly spread across the entire genome and not limited to plasmid or pathogenicity island loci. Among the pools of clinical isolates, genomic variation was concentrated adjacent to previously reported inversion and duplication differences between the SMS-3-5 isolate and the drug-susceptible laboratory strain, DH10B. SNPs that result in non-synonymous changes in gyrA (encoding the well-known S83L allele associated with fluoroquinolone resistance, mutM, ligB, and recG were unanimously conserved in every fluoroquinolone-resistant pool. Alleles of the latter three genes are tightly linked among most sequenced E. coli genomes, and had not been implicated in antibiotic resistance previously. The changes in these genes map to amino acid positions in alpha helices that are involved in DNA binding. Plasmid-encoded complementation of null strains with either allelic variant of mutM or ligB resulted in variable responses to ultraviolet light or hydrogen peroxide treatment as markers of induced DNA damage, indicating their importance in DNA metabolism and revealing a potential mechanism for fluoroquinolone resistance. Our approach uncovered evidence that additional DNA binding enzymes may contribute to fluoroquinolone resistance and further implicate environmental bacteria as a

  3. Targeted resequencing of regulatory regions at schizophrenia risk loci: Role of rare functional variants at chromatin repressive states.

    Science.gov (United States)

    González-Peñas, Javier; Amigo, Jorge; Santomé, Luis; Sobrino, Beatriz; Brenlla, Julio; Agra, Santiago; Paz, Eduardo; Páramo, Mario; Carracedo, Ángel; Arrojo, Manuel; Costas, Javier

    2016-07-01

    There is mounting evidence that regulatory variation plays an important role in genetic risk for schizophrenia. Here, we specifically search for regulatory variants at risk by sequencing promoter regions of twenty-three genes implied in schizophrenia by copy number variant or genome-wide association studies. After strict quality control, a total of 55,206bp per sample were analyzed in 526 schizophrenia cases and 516 controls from Galicia, NW Spain, using the Applied Biosystems SOLiD System. Variants were filtered based on frequency from public databases, chromatin states from the RoadMap Epigenomics Consortium at tissues relevant for schizophrenia, such as fetal brain, mid-frontal lobe, and angular gyrus, and prediction of functionality from RegulomeDB. The proportion of rare variants at polycomb repressive chromatin state at relevant tissues was higher in cases than in controls. The proportion of rare variants with predicted regulatory role was significantly higher in cases than in controls (P=0.0028, OR=1.93, 95% C.I.=1.23-3.04). Combination of information from both sources led to the identification of an excess of carriers of rare variants with predicted regulatory role located at polycomb repressive chromatin state at relevant tissues in cases versus controls (P=0.0016, OR=19.34, 95% C.I.=2.45-2495.26). The variants are located at two genes affected by the 17q12 copy number variant, LHX1 and HNF1B. These data strongly suggest that a specific epigenetic mechanism, chromatin remodeling by histone modification during early development, may be impaired in a subset of schizophrenia patients, in agreement with previous data.

  4. Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis

    KAUST Repository

    Cui, Peng

    2012-06-08

    The global features of H3K4 and H3K27 trimethylations (H3K4me3 and H3K27me3) have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. However, the bivalent domains tend towards a “winner-takes-all” approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a “winner-takes-all” principle to regulate gene expression.

  5. ngs_backbone: a pipeline for read cleaning, mapping and SNP calling using Next Generation Sequence

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    Cañizares Joaquin

    2011-06-01

    Full Text Available Abstract Background The possibilities offered by next generation sequencing (NGS platforms are revolutionizing biotechnological laboratories. Moreover, the combination of NGS sequencing and affordable high-throughput genotyping technologies is facilitating the rapid discovery and use of SNPs in non-model species. However, this abundance of sequences and polymorphisms creates new software needs. To fulfill these needs, we have developed a powerful, yet easy-to-use application. Results The ngs_backbone software is a parallel pipeline capable of analyzing Sanger, 454, Illumina and SOLiD (Sequencing by Oligonucleotide Ligation and Detection sequence reads. Its main supported analyses are: read cleaning, transcriptome assembly and annotation, read mapping and single nucleotide polymorphism (SNP calling and selection. In order to build a truly useful tool, the software development was paired with a laboratory experiment. All public tomato Sanger EST reads plus 14.2 million Illumina reads were employed to test the tool and predict polymorphism in tomato. The cleaned reads were mapped to the SGN tomato transcriptome obtaining a coverage of 4.2 for Sanger and 8.5 for Illumina. 23,360 single nucleotide variations (SNVs were predicted. A total of 76 SNVs were experimentally validated, and 85% were found to be real. Conclusions ngs_backbone is a new software package capable of analyzing sequences produced by NGS technologies and predicting SNVs with great accuracy. In our tomato example, we created a highly polymorphic collection of SNVs that will be a useful resource for tomato researchers and breeders. The software developed along with its documentation is freely available under the AGPL license and can be downloaded from http://bioinf.comav.upv.es/ngs_backbone/ or http://github.com/JoseBlanca/franklin.

  6. Development of Prediction Model and Experimental Validation in Predicting the Curcumin Content of Turmeric (Curcuma longa L.).

    Science.gov (United States)

    Akbar, Abdul; Kuanar, Ananya; Joshi, Raj K; Sandeep, I S; Mohanty, Sujata; Naik, Pradeep K; Mishra, Antaryami; Nayak, Sanghamitra

    2016-01-01

    The drug yielding potential of turmeric (Curcuma longa L.) is largely due to the presence of phyto-constituent 'curcumin.' Curcumin has been found to possess a myriad of therapeutic activities ranging from anti-inflammatory to neuroprotective. Lack of requisite high curcumin containing genotypes and variation in the curcumin content of turmeric at different agro climatic regions are the major stumbling blocks in commercial production of turmeric. Curcumin content of turmeric is greatly influenced by environmental factors. Hence, a prediction model based on artificial neural network (ANN) was developed to map genome environment interaction basing on curcumin content, soli and climatic factors from different agroclimatic regions for prediction of maximum curcumin content at various sites to facilitate the selection of suitable region for commercial cultivation of turmeric. The ANN model was developed and tested using a data set of 119 generated by collecting samples from 8 different agroclimatic regions of Odisha. The curcumin content from these samples was measured that varied from 7.2% to 0.4%. The ANN model was trained with 11 parameters of soil and climatic factors as input and curcumin content as output. The results showed that feed-forward ANN model with 8 nodes (MLFN-8) was the most suitable one with R(2) value of 0.91. Sensitivity analysis revealed that minimum relative humidity, altitude, soil nitrogen content and soil pH had greater effect on curcumin content. This ANN model has shown proven efficiency for predicting and optimizing the curcumin content at a specific site.

  7. La gestione dei flussi turistici tramite sistemi di trasporto innovativi: l’esempio di Perugia

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    Leonardo Naldini

    2010-10-01

    Full Text Available La città di Perugia ha, fin dagli anni ’70, unito alla sua capacità di attrazione turistica, dovuta al patrimonio storico ambientale plurimillenario, una volontà di affrontare il tema della mobilità urbana in maniera profondamente innovativa ed ambientalmente sostenibile. Il concetto della creazione di parcheggi di attestazione, serviti da percorsi meccanizzati di risalita verso l’acropoli, è stato recentemente ampliato dalla creazione del Minimetro, mezzo di trasporto innovativo, dal ridottissimo impatto ambientale, che unisce in soli 12 minuti, con un servizio semicontinuo, l’area di valle di Pian di Massiano, dotata di un capace parcheggio da circa 2800 posti auto, al centro cittadino, servendo diversi quartieri urbani. Tale sistema di trasporto, corridoio forte del trasporto pubblico urbano, ha fornito lo spunto per attuare una nuova modalità di gestione dei flussi turistici, che individua nel Minimetro la modalità principale di accesso al centro storico sia per i gruppi a numero ridotto, sia per i gruppi organizzati in arrivo con i bus turistici. L’organizzazione della mobilità turistica che ne è derivata costituisce un ulteriore passo in avanti lungo quell’itinerario di gestione della città che cerca di coniugare l’attrattiva turistica, con le sue ricadute in termini di crescita economica, e la salvaguardia del patrimonio storico artistico e della qualità della vita dei cittadini. Gli scenari futuri vedono un’iniezione di tecnologia in questo contesto, con l’introduzione nella gestione dei flussi turistici dei concetti di accreditamento, dell’infomobilità come supporto alle politiche urbane, del controllo unito alla fornitura di servizi georeferenziati.

  8. Shotgun metagenomic data reveals signifcant abundance but low diversity of Candidatus Scalindua marine anammox bacteria in the Arabian Sea oxygen minimum zone

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    laura eVillanueva

    2014-02-01

    Full Text Available Anaerobic ammonium oxidizing (anammox bacteria are responsible for a significant portion of the loss of fixed nitrogen from the oceans, making them important players in the global nitrogen cycle. To date, marine anammox bacteria found in both water columns and sediments worldwide belong almost exclusively to Candidatus Scalindua species. Recently the genome assembly of a marine anammox enrichment culture dominated by Candidatus Scalindua profunda became available and can now be used as a template to study metagenome data obtained from various oxygen minimum zones. Here, we sequenced genomic DNA from suspended particulate matter recovered at the upper (170 m deep and center (600 m area of the oxygen minimum zone in the Arabian Sea by SOLiD and Ion Torrent technology. The genome of Candidatus Scalindua profunda served as a template to collect reads. Based on the mapped reads marine anammox Abundance was estimated to be at least 0.4% in the upper and 1.7% in the center area. Single nucleotide variation (SNV analysis was performed to assess diversity of the Candidatus Scalindua populations. Most highly covered were the two diagnostic anammox genes hydrazine synthase (scal_01318c, hzsA and hydrazine dehydrogenase (scal_03295, hdh, while other genes involved in anammox metabolism (narGH, nirS, amtB, focA and ACS had a lower coverage but could still be assembled and analyzed. The results show that Candidatus Scalindua is abundantly present in the Arabian Sea OMZ, but that the diversity within the ecosystem is relatively low.

  9. Long term preservation of motion with artificial cervical disc implants: A comparison between cervical disc replacement and rigid fusion with cage

    Science.gov (United States)

    Cincu, Rafael; Lorente, Francisco de Asis; Gomez, Joaquin; Eiras, Jose; Agrawal, Amit

    2014-01-01

    Background: With the advancement of technologies there is more interest in the maintenance of the spine's biomechanical properties focusing on the preservation of the functional motion segment. In present article we describe our experience with 25 cases managed with artificial cervical discs with 28 Solis cage following cervical discectomy with a mean follow-up period of 7.5 year. Materials and Methods: All surgeries were performed by single surgeon from March 2004 to June 2005 with a follow-up till date. Patients with symptomatic single or multiple level diseases that had no prior cervical surgery were candidates for the study. Cohort demographics were comparable. Standardized clinical outcome measures and radiographic examinations were used at prescribed post-operative intervals to compare the treatment groups. Relief in radicular pain, cervical spine motion, and degenerative changes at follow-up were noted. Results: In a total 53 cases, the mean age in prosthesis group was 47 years (age range: 30-63 years) and mean age in cage group was 44 years (32-62 years). Mean hospital stay was 2.7 days in both the groups. At 4 weeks complete cervical movements could be achieved in 19 cases in artificial disc group. Maintenance of movement after 7.5 years was in 76% of these patients. Lordosis was maintained in all cases till date. There was no mortality or wound infection in our series. Conclusions: We conclude that artificial cervical disc could be an alternative to fixed spinal fusion as it represents the most physiological substitute of disc. However, there is need for further studies to support the use of artificial cervical disc prosthesis. PMID:25685218

  10. Fish the ChIPs: a pipeline for automated genomic annotation of ChIP-Seq data

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    Minucci Saverio

    2011-10-01

    Full Text Available Abstract Background High-throughput sequencing is generating massive amounts of data at a pace that largely exceeds the throughput of data analysis routines. Here we introduce Fish the ChIPs (FC, a computational pipeline aimed at a broad public of users and designed to perform complete ChIP-Seq data analysis of an unlimited number of samples, thus increasing throughput, reproducibility and saving time. Results Starting from short read sequences, FC performs the following steps: 1 quality controls, 2 alignment to a reference genome, 3 peak calling, 4 genomic annotation, 5 generation of raw signal tracks for visualization on the UCSC and IGV genome browsers. FC exploits some of the fastest and most effective tools today available. Installation on a Mac platform requires very basic computational skills while configuration and usage are supported by a user-friendly graphic user interface. Alternatively, FC can be compiled from the source code on any Unix machine and then run with the possibility of customizing each single parameter through a simple configuration text file that can be generated using a dedicated user-friendly web-form. Considering the execution time, FC can be run on a desktop machine, even though the use of a computer cluster is recommended for analyses of large batches of data. FC is perfectly suited to work with data coming from Illumina Solexa Genome Analyzers or ABI SOLiD and its usage can potentially be extended to any sequencing platform. Conclusions Compared to existing tools, FC has two main advantages that make it suitable for a broad range of users. First of all, it can be installed and run by wet biologists on a Mac machine. Besides it can handle an unlimited number of samples, being convenient for large analyses. In this context, computational biologists can increase reproducibility of their ChIP-Seq data analyses while saving time for downstream analyses. Reviewers This article was reviewed by Gavin Huttley, George

  11. Niabella hibiscisoli sp. nov., isolated from soil of a Rose of Sharon garden.

    Science.gov (United States)

    Ngo, Hien T T; Trinh, Huan; Yan, Zheng-Fei; Moya, Gabriela; Kook, MooChang; Yi, Tae-Hoo

    2016-10-21

    A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN5.5T, was isolated from soil of a Rose of Sharon garden in Daejeon, South Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN5.5T was found to be most closely related to Niabella yanshanensis CCBAU 05354T (97.7 % sequence similarity), Niabella ginsengisoli GR10-1T (97.0 %), Niabella terrae ICM 1-15T (96.0 %), Niabella soli DSM 19437T (95.7 %), and Niabella aquatica RP-2T (95.6 %). The DNA-DNA relatedness between strain THG-DN5.5T and its phylogenetically closest neighbours was below 50.0 %. The DNA G+C content was 43.1 mol%. The major polar lipid was found to be phosphati¬dylethanolamine. The major fatty acids were identified as C16:0, iso-C15:0, iso-C15:1 G, and iso-C17:0 3OH. MK-7 was the only menaquinone present. These data supported the affiliation of strain THG-DN5.5T to the genus Niabella. Strain THG-DN5.5T was distinguished from related Niabella species by physiological and biochemical tests. In this study, the novel isolate represents a novel species, for which the name Niabella hibiscisoli sp. nov. is proposed, with THG-DN5.5T as the type strain (= KACC 18857T = CCTCC AB 2016086T).

  12. High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.

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    Fermín I Milagro

    Full Text Available INTRODUCTION: MicroRNAs (miRNAs are being increasingly studied in relation to energy metabolism and body composition homeostasis. Indeed, the quantitative analysis of miRNAs expression in different adiposity conditions may contribute to understand the intimate mechanisms participating in body weight control and to find new biomarkers with diagnostic or prognostic value in obesity management. OBJECTIVE: The aim of this study was the search for miRNAs in blood cells whose expression could be used as prognostic biomarkers of weight loss. METHODS: Ten Caucasian obese women were selected among the participants in a weight-loss trial that consisted in following an energy-restricted treatment. Weight loss was considered unsuccessful when 5% (responders. At baseline, total miRNA isolated from peripheral blood mononuclear cells (PBMC was sequenced with SOLiD v4. The miRNA sequencing data were validated by RT-PCR. RESULTS: Differential baseline expression of several miRNAs was found between responders and non-responders. Two miRNAs were up-regulated in the non-responder group (mir-935 and mir-4772 and three others were down-regulated (mir-223, mir-224 and mir-376b. Both mir-935 and mir-4772 showed relevant associations with the magnitude of weight loss, although the expression of other transcripts (mir-874, mir-199b, mir-766, mir-589 and mir-148b also correlated with weight loss. CONCLUSIONS: This research addresses the use of high-throughput sequencing technologies in the search for miRNA expression biomarkers in obesity, by determining the miRNA transcriptome of PBMC. Basal expression of different miRNAs, particularly mir-935 and mir-4772, could be prognostic biomarkers and may forecast the response to a hypocaloric diet.

  13. Evaluation of biostimulation and Tween 80 addition for the bioremediation of long-term DDT-contaminated soil.

    Science.gov (United States)

    Betancur-Corredor, Bibiana; Pino, Nancy J; Cardona, Santiago; Peñuela, Gustavo A

    2015-02-01

    The bioremediation of a long-term contaminated soil through biostimulation and surfactant addition was evaluated. The concentrations of 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT) and its metabolites 1,1-dichloro-2,2-bis(4-chlorophenyl) ethane (DDD) and 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (DDE) were monitored during an 8-week remediation process. Physicochemical characterization of the treated soil was performed before and after the bioremediation process. The isolation and identification of predominant microorganisms during the remediation process were also carried out. The efficiency of detoxification was evaluated after each bioremediation protocol. Humidity and pH and the heterotrophic microorganism count were monitored weekly. The DDT concentration was reduced by 79% after 8 weeks via biostimulation with surfactant addition (B+S) and 94.3% via biostimulation alone (B). Likewise, the concentrations of the metabolites DDE and DDD were reduced to levels below the quantification limits. The microorganisms isolated during bioremediation were identified as Bacillus thuringiensis, Flavobacterium sp., Cuprivadius sp., Variovorax soli, Phenylobacterium sp. and Lysobacter sp., among others. Analysis with scanning electron microscopy (SEM) allowed visualization of the colonization patterns of soil particles. The toxicity of the soil before and after bioremediation was evaluated using Vibrio fischeri as a bioluminescent sensor. A decrease in the toxic potential of the soil was verified by the increase of the concentration/effect relationship EC50 to 26.9% and 27.2% for B+S and B, respectively, compared to 0.4% obtained for the soil before treatment and 2.5% by natural attenuation after 8 weeks of treatment.

  14. The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

    Science.gov (United States)

    Leão, Sylvia Cardoso; Matsumoto, Cristianne Kayoko; Carneiro, Adriana; Ramos, Rommel Thiago; Nogueira, Christiane Lourenço; Junior, James Daltro Lima; Lima, Karla Valéria; Lopes, Maria Luiza; Schneider, Horacio; Azevedo, Vasco Ariston; da Costa da Silva, Artur

    2013-01-01

    Background An extended outbreak of mycobacterial surgical infections occurred in Brazil during 2004–2008. Most infections were caused by a single strain of Mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpoB sequevar and two highly similar pulsed-field gel electrophoresis (PFGE) patterns differentiated by the presence of a ∼50 kb band. The nature of this band was investigated. Methodology/Principal Findings Genomic sequencing of the prototype outbreak isolate INCQS 00594 using the SOLiD platform demonstrated the presence of a 56,264-bp circular plasmid, designated pMAB01. Identity matrices, genetic distances and phylogeny analyses indicated that pMAB01 belongs to the broad-host-range plasmid subgroup IncP-1β and is highly related to BRA100, pJP4, pAKD33 and pB10. The presence of pMAB01-derived sequences in 41 M. abscessus subsp. bolletii isolates was evaluated using PCR, PFGE and Southern blot hybridization. Sixteen of the 41 isolates showed the presence of the plasmid. The plasmid was visualized as a ∼50-kb band using PFGE and Southern blot hybridization in 12 isolates. The remaining 25 isolates did not exhibit any evidence of this plasmid. The plasmid was successfully transferred to Escherichia coli by conjugation and transformation. Lateral transfer of pMAB01 to the high efficient plasmid transformation strain Mycobacterium smegmatis mc2155 could not be demonstrated. Conclusions/Significance The occurrence of a broad-host-range IncP-1β plasmid in mycobacteria is reported for the first time. Thus, genetic exchange could result in the emergence of specific strains that might be better adapted to cause human disease. PMID:23565273

  15. Photospheric and coronal magnetic fields in six magnetographs. I. Consistent evolution of the bashful ballerina

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    Virtanen, Ilpo; Mursula, Kalevi

    2016-06-01

    Aims: We study the long-term evolution of photospheric and coronal magnetic fields and the heliospheric current sheet (HCS), especially its north-south asymmetry. Special attention is paid to the reliability of the six data sets used in this study and to the consistency of the results based on these data sets. Methods: We use synoptic maps constructed from Wilcox Solar Observatory (WSO), Mount Wilson Observatory (MWO), Kitt Peak (KP), SOLIS, SOHO/MDI, and SDO/HMI measurements of the photospheric field and the potential field source surface (PFSS) model. Results: The six data sets depict a fairly similar long-term evolution of magnetic fields and the heliospheric current sheet, including polarity reversals and hemispheric asymmetry. However, there are time intervals of several years long, when first KP measurements in the 1970s and 1980s, and later WSO measurements in the 1990s and early 2000s, significantly deviate from the other simultaneous data sets, reflecting likely errors at these times. All of the six magnetographs agree on the southward shift of the heliospheric current sheet (the so-called bashful ballerina phenomenon) in the declining to minimum phase of the solar cycle during a few years of the five included cycles. We show that during solar cycles 20-22, the southward shift of the HCS is mainly due to the axial quadrupole term, reflecting the stronger magnetic field intensity at the southern pole during these times. During cycle 23 the asymmetry is less persistent and mainly due to higher harmonics than the quadrupole term. Currently, in the early declining phase of cycle 24, the HCS is also shifted southward and is mainly due to the axial quadrupole as for most earlier cycles. This further emphasizes the special character of the global solar field during cycle 23.

  16. Integrating sequencing technologies in personal genomics: optimal low cost reconstruction of structural variants.

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    Jiang Du

    2009-07-01

    Full Text Available The goal of human genome re-sequencing is obtaining an accurate assembly of an individual's genome. Recently, there has been great excitement in the development of many technologies for this (e.g. medium and short read sequencing from companies such as 454 and SOLiD, and high-density oligo-arrays from Affymetrix and NimbelGen, with even more expected to appear. The costs and sensitivities of these technologies differ considerably from each other. As an important goal of personal genomics is to reduce the cost of re-sequencing to an affordable point, it is worthwhile to consider optimally integrating technologies. Here, we build a simulation toolbox that will help us optimally combine different technologies for genome re-sequencing, especially in reconstructing large structural variants (SVs. SV reconstruction is considered the most challenging step in human genome re-sequencing. (It is sometimes even harder than de novo assembly of small genomes because of the duplications and repetitive sequences in the human genome. To this end, we formulate canonical problems that are representative of issues in reconstruction and are of small enough scale to be computationally tractable and simulatable. Using semi-realistic simulations, we show how we can combine different technologies to optimally solve the assembly at low cost. With mapability maps, our simulations efficiently handle the inhomogeneous repeat-containing structure of the human genome and the computational complexity of practical assembly algorithms. They quantitatively show how combining different read lengths is more cost-effective than using one length, how an optimal mixed sequencing strategy for reconstructing large novel SVs usually also gives accurate detection of SNPs/indels, how paired-end reads can improve reconstruction efficiency, and how adding in arrays is more efficient than just sequencing for disentangling some complex SVs. Our strategy should facilitate the sequencing of

  17. De novo assembly and characterization of the carrot transcriptome reveals novel genes, new markers, and genetic diversity

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    Matvienko Marta

    2011-08-01

    Full Text Available Abstract Background Among next generation sequence technologies, platforms such as Illumina and SOLiD produce short reads but with higher coverage and lower cost per sequenced nucleotide than 454 or Sanger. A challenge now is to develop efficient strategies to use short-read length platforms for de novo assembly and marker development. The scope of this study was to develop a de novo assembly of carrot ESTs from multiple genotypes using the Illumina platform, and to identify polymorphisms. Results A de novo assembly of transcriptome sequence from four genetic backgrounds produced 58,751 contigs and singletons. Over 50% of these assembled sequences were annotated allowing detection of transposable elements and new carrot anthocyanin genes. Presence of multiple genetic backgrounds in our assembly allowed the identification of 114 computationally polymorphic SSRs, and 20,058 SNPs at a depth of coverage of 20× or more. Polymorphisms were predominantly between inbred lines except for the cultivated x wild RIL pool which had high intra-sample polymorphism. About 90% and 88% of tested SSR and SNP primers amplified a product, of which 70% and 46%, respectively, were of the expected size. Out of verified SSR and SNP markers 84% and 82% were polymorphic. About 25% of SNPs genotyped were polymorphic in two diverse mapping populations. Conclusions This study confirmed the potential of short read platforms for de novo EST assembly and identification of genetic polymorphisms in carrot. In addition we produced the first large-scale transcriptome of carrot, a species lacking genomic resources.

  18. Rhodanobacter spathiphylli sp. nov., a gammaproteobacterium isolated from the roots of Spathiphyllum plants grown in a compost-amended potting mix.

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    De Clercq, Deborah; Van Trappen, Stefanie; Cleenwerck, Ilse; Ceustermans, An; Swings, Jean; Coosemans, Jozef; Ryckeboer, Jaak

    2006-08-01

    Three Gram-negative, yellow-pigmented strains were isolated from the rhizospheres of Spathiphyllum plants grown in a compost-amended potting mix. The strains showed biological control activity towards the root-rot plant pathogen Cylindrocladium spathiphylli, and were characterized to determine their taxonomic position. Cells of the strains were non-motile rods, and the strains were oxidase- and catalase-positive and unable to ferment most sugars tested. The three strains showed differences in growth temperature range, optimal growth temperature and some biochemical reactions. The majority of the fatty acids were branched, and large amounts of 15 : 0 iso and 17 : 1 iso omega9c were present. The 16S rRNA gene sequence (1,497 bp) of strain B39(T) showed the highest level of similarity (98.5 %) to that of Rhodanobacter fulvus IAM 15025(T), followed by Rhodanobacter lindaniclasticus LMG 18385(T) (96.0 %; strain no longer extant), Dyella koreensis CCUG 50883(T) (96.4 %), Dyella japonica DSM 16301(T) (96.3 %), Frateuria aurantia LMG 1558(T) (96.2 %) and Fulvimonas soli LMG 19981(T) (95.9 %). Less than 90 % 16S rRNA gene sequence similarity was observed for other members of the Gammaproteobacteria. The mean DNA-DNA reassociation value for the three strains was 100 % and was 25 % when the strains were compared with DNA from R. fulvus LMG 23003(T). The strains had a mean DNA G+C content of 67.6 mol%. On the basis of their phylogenetic, genomic and phenotypic properties, the three strains represent a novel species within the genus Rhodanobacter, for which the name Rhodanobacter spathiphylli sp. nov. is proposed. The type strain is strain B39(T) (=LMG 23181(T)=DSM 17631(T)).

  19. Ca II 854.2 nm Spectromagnetograms: A Powerful Chromospheric Diagnostic

    Science.gov (United States)

    Harvey, J. W.; Bertello, Luca; Branston, D.; Britanik, J.; Bulau, S.; Cole, L.; Gosain, Sanjay; Harker, Brian; Jones, Harrison P.; Marble, A.; Martinez Pillet, V.; Pevtsov, A.; Schramm, K.; Streander, Kim; Villegas, H.

    2016-05-01

    The transition from physical dominance by plasma flows in the photosphere to magnetic pressure in the solar chromosphere motivates as many diagnostic observations as possible across this important region. Among the few ground-accessible spectral lines formed within the chromosphere, the Ca II 854.2 nm line has the desirable properties of presence everywhere on the solar disk, Zeeman sensitivity, and narrow line width. Mapped observations of circular polarization within this line (spectromagnetograms) have been made at NSO infrequently since 1974, with regular daily full-disk observations starting in August 1996. Full-disk spectral observations of the complete Stokes polarization vector are now being made regularly since November 2015. It is not easy to estimate chromospheric magnetic field properties from the 854.2 nm line profile polarization. To provide rough quick-look vector field maps we found that the weak-field approximation provides a fair first estimate of the line-of-sight component but appears to be too simple to interpret the transverse magnetic field from frequently asymmetric, linearly-polarized line profiles. More realistic estimates of the chromospheric vector field, short of extremely lengthy, full 3D, non-local radiative transfer inversions, are being investigated. We briefly introduce recent instrumental modifications and observational characteristics, sample observations, and results concerning the expansion of the chromospheric field with increasing height, the presence of large areas of weak, nearly horizontal fields, and field estimates in plages, sunspots, flares, filaments, and filament channels. The Stokes spectra will be freely available to the community.This work utilizes SOLIS data obtained by the NSO Integrated Synoptic Program (NISP), managed by the National Solar Observatory, which is operated by the Association of Universities for Research in Astronomy (AURA), Inc. under a cooperative agreement with the National Science Foundation.

  20. Seven-year survival after pancreaticoduodenectomy for early recurrent renal cell carcinoma involving the duodenum A case report.

    Science.gov (United States)

    Cannistra', Marco; Ruggiero, Michele; Bonaiuto, Elisabetta; Vaccarisi, Sebastiano; Naso, Agostino; Grande, Raffaele; Nardo, Bruno

    2015-12-29

    Il cancro del rene rappresenta la 13a forma di tumore a livello mondiale e risulta incrementata, dal punto di vista epidemiologico, di circa il 2% annuo nelle ultime due decadi. Studi recenti hanno mostrato un numero complessivo di 65,150 nuovi casi di cancro renale diagnosticato nei paesi occidentali e circa 13.680 morti. Il carcinoma a cellule renali rappresenta approssimativamente il 90% di tutte le forme di cancro renale e presenta un tasso di mortalità di circa 4 soggetti ogni 100000 abitanti per anno. Il carcinoma a cellule renali ha, spesso, un particolare corteo sintomatologico caratterizzato da dolore addominale, masse addominali ed ematuria. Caratteristico è il fatto che circa il 25-30% dei pazienti, all’atto della diagnosi, presentano già metastasti a distanza; un ulteriore 30-50%, inoltre, svilupperanno metastasi nel corso della malattia, anche dopo molti anni dall’operazione chirurgica di nefrectomia radicale. Il Cancro del Rene a Cellule Chiare metastatizza in genere per via linfatica, ematogena, transperitoneale o per invasione diretta, ed i siti di metastasi più comuni sono il polmone ed i linfonodi, seguiti dalle ossa e dal fegato. Tra tutte le possibili localizzazioni ai segmenti addominali, il duodeno rappresenta la sede meno colpita: quando coinvolta, viene, in genere, invasa la regione periampollare ed il bulbo del duodeno. Tali localizzazioni danno segno di sè, spesso tardivamente, con sanguinamenti (ematemesi) o ostruzioni del tratto gastrointestinale superiore (vomito). Il nostro caso clinico descrive un carcinoma a cellule renali che si presentava, dopo soli 5 mesi dalla nefrectomia radicale destra, con sanguinamento del tratto gastrointestinale superiore e metastasi a carico del duodeno, e che è stato trattato chirurgicamente con duodenocefalopancreasectomia. L’intervento chirurgico, noto per essere altamente demolitivo ed associato ad elevata morbilità, applicato a recidiva precoce di cancro, ha garantito un ottimo risultato

  1. Discovery of posttranscriptional regulatory RNAs using next generation sequencing technologies.

    Science.gov (United States)

    Gelderman, Grant; Contreras, Lydia M

    2013-01-01

    Next generation sequencing (NGS) has revolutionized the way by which we engineer metabolism by radically altering the path to genome-wide inquiries. This is due to the fact that NGS approaches offer several powerful advantages over traditional methods that include the ability to fully sequence hundreds to thousands of genes in a single experiment and simultaneously detect homozygous and heterozygous deletions, alterations in gene copy number, insertions, translocations, and exome-wide substitutions that include "hot-spot mutations." This chapter describes the use of these technologies as a sequencing technique for transcriptome analysis and discovery of regulatory RNA elements in the context of three main platforms: Illumina HiSeq, 454 pyrosequencing, and SOLiD sequencing. Specifically, this chapter focuses on the use of Illumina HiSeq, since it is the most widely used platform for RNA discovery and transcriptome analysis. Regulatory RNAs have now been found in all branches of life. In bacteria, noncoding small RNAs (sRNAs) are involved in highly sophisticated regulatory circuits that include quorum sensing, carbon metabolism, stress responses, and virulence (Gorke and Vogel, Gene Dev 22:2914-2925, 2008; Gottesman, Trends Genet 21:399-404, 2005; Romby et al., Curr Opin Microbiol 9:229-236, 2006). Further characterization of the underlying regulation of gene expression remains poorly understood given that it is estimated that over 60% of all predicted genes remain hypothetical and the 5' and 3' untranslated regions are unknown for more than 90% of the genes (Siegel et al., Trends Parasitol 27:434-441, 2011). Importantly, manipulation of the posttranscriptional regulation that occurs at the level of RNA stability and export, trans-splicing, polyadenylation, protein translation, and protein stability via untranslated regions (Clayton, EMBO J 21:1881-1888, 2002; Haile and Papadopoulou, Curr Opin Microbiol 10:569-577, 2007) could be highly beneficial to metabolic

  2. Sunspot positions, areas, and group tilt angles for 1611-1631 from observations by Christoph Scheiner

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    Arlt, R.; Senthamizh Pavai, V.; Schmiel, C.; Spada, F.

    2016-11-01

    Aims: Digital images of observations printed in the books Rosa Ursina sive solis and Prodromus pro sole mobili by Christoph Scheiner, as well as the drawings from Scheiner's letters to Marcus Welser, are analysed to obtain information on the positions and sizes of sunspots that appeared before the Maunder minimum. Methods: In most cases, the given orientation of the ecliptic is used to set up the heliographic coordinate system for the drawings. Positions and sizes are measured manually on screen. Very early drawings have no indication of their orientation. A rotational matching using common spots of adjacent days is used in some cases, while in other cases, the assumption that images were aligned with a zenith-horizon coordinate system appeared to be the most probable. Results: In total, 8167 sunspots were measured. A distribution of sunspot latitudes versus time (butterfly diagram) is obtained for Scheiner's observations. The observations of 1611 are very inaccurate, the drawings of 1612 have at least an indication of their orientation, while the remaining part of the spot positions from 1618-1631 have good to very good accuracy. We also computed 697 tilt angles of apparently bipolar sunspot groups observed in the period 1618-1631. We find that the average tilt angle of nearly 4 degrees is not significantly different from 20th-century values. Data on the sunspot position and area are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/595/A104

  3. Development of Prediction Model and Experimental Validation in Predicting the Curcumin Content of Turmeric (Curcuma longa L.)

    Science.gov (United States)

    Akbar, Abdul; Kuanar, Ananya; Joshi, Raj K.; Sandeep, I. S.; Mohanty, Sujata; Naik, Pradeep K.; Mishra, Antaryami; Nayak, Sanghamitra

    2016-01-01

    The drug yielding potential of turmeric (Curcuma longa L.) is largely due to the presence of phyto-constituent ‘curcumin.’ Curcumin has been found to possess a myriad of therapeutic activities ranging from anti-inflammatory to neuroprotective. Lack of requisite high curcumin containing genotypes and variation in the curcumin content of turmeric at different agro climatic regions are the major stumbling blocks in commercial production of turmeric. Curcumin content of turmeric is greatly influenced by environmental factors. Hence, a prediction model based on artificial neural network (ANN) was developed to map genome environment interaction basing on curcumin content, soli and climatic factors from different agroclimatic regions for prediction of maximum curcumin content at various sites to facilitate the selection of suitable region for commercial cultivation of turmeric. The ANN model was developed and tested using a data set of 119 generated by collecting samples from 8 different agroclimatic regions of Odisha. The curcumin content from these samples was measured that varied from 7.2% to 0.4%. The ANN model was trained with 11 parameters of soil and climatic factors as input and curcumin content as output. The results showed that feed-forward ANN model with 8 nodes (MLFN-8) was the most suitable one with R2 value of 0.91. Sensitivity analysis revealed that minimum relative humidity, altitude, soil nitrogen content and soil pH had greater effect on curcumin content. This ANN model has shown proven efficiency for predicting and optimizing the curcumin content at a specific site. PMID:27766103

  4. Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.

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    Ândrea Ribeiro-dos-Santos

    Full Text Available BACKGROUND: While microRNAs (miRNAs play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. METHODOLOGY/PRINCIPAL FINDINGS: A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P<0.05. Six miRNAs showed a low variable pattern of expression (miR-29b, miR-29c, miR-19b, miR-31, miR-148a, miR-451 and could be considered part of the expression pattern of the healthy gastric tissue. CONCLUSIONS/SIGNIFICANCE: This study aimed to validate normal miRNA profiles of human gastric tissue to establish a reference profile for healthy individuals. Determining the regulatory processes acting in the stomach will be important in the fight against gastric cancer, which is the second-leading cause of cancer mortality worldwide.

  5. Trasformazione urbana verso la mobilità dolce: il caso di Istanbul

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    Emilia Giovanna Trifiletti

    2008-12-01

    Full Text Available Nell’articolo si descrivono le recenti trasformazioni urbane della città di Istanbul in Turchia in relazione all’implementazione di aree pedonali e all’utilizzo di mobilità dolce. Il sistema trasportistico è stato ampliato ed integrato di recente ed in tempi molto brevi: nei soli ultimi due anni è stata potenziata la linea tranviaria, sono state inaugurate una funicolare ed una teleferica ed è stata realizzata una stazione di interscambio gomma-ferro, ponendo grande attenzione all’integrazione del trasporto su ferro e/o gomma con la mobilità pedonale. Allo stesso modo sono state implementate le linee marittime che collegano le diverse sponde della città ed integrate alle linee su ferro e su gomma. Lo sviluppo e la riqualificazione della città sono dunque stati guidati dalla trasformazione del sistema trasportistico che ha rappresentato il vero motore della riqualificazione urbana. L’antico quartiere di Sultanahmet è stato interamente riqualificato e collegato, attraverso la linea tranviaria veloce che lo attraversa interamente, al resto della città fino ai quartieri più periferici. Allo stesso modo nel quartiere di Beyoglu che, dopo l’indipendenza era caduto in totale rovina, ha riacquistato l’antica eleganza che lo connotava. Il viale dell’Indipendenza (antica Rue De Pera che attraversa tutto il quartiere è stato reso pedonale e con un tram vintage che percorre tutto il viale sono stati collegati il nodo di interscambio Taksim, dotato di metropolitana e funicolare, con il nodo di interscambio Tünel, dotato di tram veloce e trasporto su gomma. Nell’articolo si è inteso evidenziare come la riqualificazione urbana della città sia stata guidata da interventi integrati trasporto/territorio e come i risultati ottenuti, nel caso-studio, siano stati anche il frutto di politiche attente alla sicurezza sociale.

  6. RNA sequencing of the exercise transcriptome in equine athletes.

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    Stefano Capomaccio

    Full Text Available The horse is an optimal model organism for studying the genomic response to exercise-induced stress, due to its natural aptitude for athletic performance and the relative homogeneity of its genetic and environmental backgrounds. Here, we applied RNA-sequencing analysis through the use of SOLiD technology in an experimental framework centered on exercise-induced stress during endurance races in equine athletes. We monitored the transcriptional landscape by comparing gene expression levels between animals at rest and after competition. Overall, we observed a shift from coding to non-coding regions, suggesting that the stress response involves the differential expression of not annotated regions. Notably, we observed significant post-race increases of reads that correspond to repeats, especially the intergenic and intronic L1 and L2 transposable elements. We also observed increased expression of the antisense strands compared to the sense strands in intronic and regulatory regions (1 kb up- and downstream of the genes, suggesting that antisense transcription could be one of the main mechanisms for transposon regulation in the horse under stress conditions. We identified a large number of transcripts corresponding to intergenic and intronic regions putatively associated with new transcriptional elements. Gene expression and pathway analysis allowed us to identify several biological processes and molecular functions that may be involved with exercise-induced stress. Ontology clustering reflected mechanisms that are already known to be stress activated (e.g., chemokine-type cytokines, Toll-like receptors, and kinases, as well as "nucleic acid binding" and "signal transduction activity" functions. There was also a general and transient decrease in the global rates of protein synthesis, which would be expected after strenuous global stress. In sum, our network analysis points toward the involvement of specific gene clusters in equine exercise

  7. RNA sequencing of the exercise transcriptome in equine athletes.

    Science.gov (United States)

    Capomaccio, Stefano; Vitulo, Nicola; Verini-Supplizi, Andrea; Barcaccia, Gianni; Albiero, Alessandro; D'Angelo, Michela; Campagna, Davide; Valle, Giorgio; Felicetti, Michela; Silvestrelli, Maurizio; Cappelli, Katia

    2013-01-01

    The horse is an optimal model organism for studying the genomic response to exercise-induced stress, due to its natural aptitude for athletic performance and the relative homogeneity of its genetic and environmental backgrounds. Here, we applied RNA-sequencing analysis through the use of SOLiD technology in an experimental framework centered on exercise-induced stress during endurance races in equine athletes. We monitored the transcriptional landscape by comparing gene expression levels between animals at rest and after competition. Overall, we observed a shift from coding to non-coding regions, suggesting that the stress response involves the differential expression of not annotated regions. Notably, we observed significant post-race increases of reads that correspond to repeats, especially the intergenic and intronic L1 and L2 transposable elements. We also observed increased expression of the antisense strands compared to the sense strands in intronic and regulatory regions (1 kb up- and downstream) of the genes, suggesting that antisense transcription could be one of the main mechanisms for transposon regulation in the horse under stress conditions. We identified a large number of transcripts corresponding to intergenic and intronic regions putatively associated with new transcriptional elements. Gene expression and pathway analysis allowed us to identify several biological processes and molecular functions that may be involved with exercise-induced stress. Ontology clustering reflected mechanisms that are already known to be stress activated (e.g., chemokine-type cytokines, Toll-like receptors, and kinases), as well as "nucleic acid binding" and "signal transduction activity" functions. There was also a general and transient decrease in the global rates of protein synthesis, which would be expected after strenuous global stress. In sum, our network analysis points toward the involvement of specific gene clusters in equine exercise-induced stress, including

  8. First Use of Synoptic Vector Magnetograms for Global Nonlinear, Force-Free Coronal Magnetic Field Models

    Science.gov (United States)

    Tadesse, T.; Wiegelmann, T.; Gosain, S.; MacNeice, P.; Pevtsov, A. A.

    2014-01-01

    Context. The magnetic field permeating the solar atmosphere is generally thought to provide the energy for much of the activity seen in the solar corona, such as flares, coronal mass ejections (CMEs), etc. To overcome the unavailability of coronal magnetic field measurements, photospheric magnetic field vector data can be used to reconstruct the coronal field. Currently, there are several modelling techniques being used to calculate three-dimensional field lines into the solar atmosphere. Aims. For the first time, synoptic maps of a photospheric-vector magnetic field synthesized from the vector spectromagnetograph (VSM) on Synoptic Optical Long-term Investigations of the Sun (SOLIS) are used to model the coronal magnetic field and estimate free magnetic energy in the global scale. The free energy (i.e., the energy in excess of the potential field energy) is one of the main indicators used in space weather forecasts to predict the eruptivity of active regions. Methods. We solve the nonlinear force-free field equations using an optimization principle in spherical geometry. The resulting threedimensional magnetic fields are used to estimate the magnetic free energy content E(sub free) = E(sub nlfff) - E(sub pot), which is the difference of the magnetic energies between the nonpotential field and the potential field in the global solar corona. For comparison, we overlay the extrapolated magnetic field lines with the extreme ultraviolet (EUV) observations by the atmospheric imaging assembly (AIA) on board the Solar Dynamics Observatory (SDO). Results. For a single Carrington rotation 2121, we find that the global nonlinear force-free field (NLFFF) magnetic energy density is 10.3% higher than the potential one. Most of this free energy is located in active regions.

  9. A Comprehensive Transcriptomic Analysis of Infant and Adult Mouse Ovary

    Directory of Open Access Journals (Sweden)

    Linlin Pan

    2014-10-01

    Full Text Available Ovary development is a complex process involving numerous genes. A well-developed ovary is essential for females to keep fertility and reproduce offspring. In order to gain a better insight into the molecular mechanisms related to the process of mammalian ovary development, we performed a comparative transcriptomic analysis on ovaries isolated from infant and adult mice by using next-generation sequencing technology (SOLiD. We identified 15,454 and 16,646 transcriptionally active genes at the infant and adult stage, respectively. Among these genes, we also identified 7021 differentially expressed genes. Our analysis suggests that, in general, the adult ovary has a higher level of transcriptomic activity. However, it appears that genes related to primordial follicle development, such as those encoding Figla and Nobox, are more active in the infant ovary, whereas expression of genes vital for follicle development, such as Gdf9, Bmp4 and Bmp15, is upregulated in the adult. These data suggest a dynamic shift in gene expression during ovary development and it is apparent that these changes function to facilitate follicle maturation, when additional functional gene studies are considered. Furthermore, our investigation has also revealed several important functional pathways, such as apoptosis, MAPK and steroid biosynthesis, that appear to be much more active in the adult ovary compared to those of the infant. These findings will provide a solid foundation for future studies on ovary development in mice and other mammals and help to expand our understanding of the complex molecular and cellular events that occur during postnatal ovary development.

  10. Agronomic Potential of Partially Acidulated Rock Phosphates in Acid Soils of Subtropical China

    Institute of Scientific and Technical Information of China (English)

    XIONGLI-MING; B.TRUONG; 等

    1995-01-01

    A glasshouse experiment was conducted to evaluate the agronomic potential of four partially acidulated rock phosphates(PARP) in three representative solis sampled from subtripical China.The PARPs were manufactured by attacking a moderately reactive phosphate rock either with sulfuric acid alone or with combination of sulfuric and phosphoric acids at 30 or 60 percent of acidulation.Shoot dry weight and P accumulation of six successive cuttings of ryegrass were used to compare the agronomic potential of these fertilizers with that of the raw rock phosphate(RP) and monocalcium phosphate (MCP).Results indicated that the effectiveness of various phosphates was determined both by the solubility of the phosphates and by the acidity and P-fixing capacity of the soils.The higher the watersoluble P contained,the better the effectiveness of the fertilizer was.Although plant P accumulation of PARP treatments was constantly lower than that of MCP treatment,some PARPs could still get a dry matter production similar to that of MCP treatment.PARP SP60,which was acidulated with a mixture of sulfuric acid and phosphoric acid at 60 percent of acidulation and contained the highest soluble,P,was as effective as MCP in terms of dry matter production on all the soils.S60 and C1 which were both acidulated with sulfuric acid with the former at 60 percent of acidulation and the latter at 30 percent but with a further addition of monoammonium phosphate,were more than 80 percent as efective as MCP,Raw RP also showed a reasonable effectiveness which increased with soil acidity.It was suggested from the study that some of these APRPs could be expected to have a comparable field performance as soluble P fertilizers in the acid soil regions.

  11. Characterization of single-nucleotide variation in Indian-origin rhesus macaques (Macaca mulatta

    Directory of Open Access Journals (Sweden)

    Wheeler David A

    2011-06-01

    Full Text Available Abstract Background Rhesus macaques are the most widely utilized nonhuman primate model in biomedical research. Previous efforts have validated fewer than 900 single nucleotide polymorphisms (SNPs in this species, which limits opportunities for genetic studies related to health and disease. Extensive information about SNPs and other genetic variation in rhesus macaques would facilitate valuable genetic analyses, as well as provide markers for genome-wide linkage analysis and the genetic management of captive breeding colonies. Results We used the available rhesus macaque draft genome sequence, new sequence data from unrelated individuals and existing published sequence data to create a genome-wide SNP resource for Indian-origin rhesus monkeys. The original reference animal and two additional Indian-origin individuals were resequenced to low coverage using SOLiD™ sequencing. We then used three strategies to validate SNPs: comparison of potential SNPs found in the same individual using two different sequencing chemistries, and comparison of potential SNPs in different individuals identified with either the same or different sequencing chemistries. Our approach validated approximately 3 million SNPs distributed across the genome. Preliminary analysis of SNP annotations suggests that a substantial number of these macaque SNPs may have functional effects. More than 700 non-synonymous SNPs were scored by Polyphen-2 as either possibly or probably damaging to protein function and these variants now constitute potential models for studying functional genetic variation relevant to human physiology and disease. Conclusions Resequencing of a small number of animals identified greater than 3 million SNPs. This provides a significant new information resource for rhesus macaques, an important research animal. The data also suggests that overall genetic variation is high in this species. We identified many potentially damaging non-synonymous coding SNPs

  12. Solar magnetic activity cycles, coronal potential field models and eruption rates

    Science.gov (United States)

    Petrie, Gordon

    2013-07-01

    We study the evolution of the observed photospheric magnetic field and the modeled global coronal magnetic field during the past 3 1/2 solar activity cycles observed since the mid-1970s. We use synoptic magnetograms and extrapolated potential-field models based on longitudinal full-disk photospheric magnetograms from the NSO's three magnetographs at Kitt Peak, the Synoptic Optical Long-term Investigations of the Sun (SOLIS) vector spectro-magnetograph (VSM), the spectro-magnetograph and the 512-channel magnetograph instruments, and from the U. Stanford's Wilcox Solar Observatory. The associated multipole field components are used to study the dominant length scales and symmetries of the coronal field. Of the axisymmetric multipoles, only the dipole and octupole follow the poles whereas the higher orders follow the activity cycle. All non-axisymmetric multipole strengths are well correlated with the activity cycle. The axial dipole and octupole are the largest contributors to the global field except while the polar fields are reversing. This influence of the polar fields extends to modulating eruption rates. According to the Computer Aided CME Tracking (CACTus), Solar Eruptive Event Detection System (SEEDS), and Nobeyama radioheliograph prominence eruption catalogs, the rate of solar eruptions is found to be systematically higher for active years between 2003-2012 than for those between 1997-2002. This behavior appears to be connected with the weakness of the late-cycle 23 polar fields as suggested by Luhmann. We see evidence that the process of cycle 24 field reversal is well advanced at both poles.

  13. Variants in microRNA genes in familial papillary thyroid carcinoma.

    Science.gov (United States)

    Tomsic, Jerneja; Fultz, Rebecca; Liyanarachchi, Sandya; Genutis, Luke K; Wang, Yanqiang; Li, Wei; Volinia, Stefano; Jazdzewski, Krystian; He, Huiling; Wakely, Paul E; Senter, Leigha; de la Chapelle, Albert

    2017-01-24

    Papillary Thyroid Carcinoma (PTC) displays one of the highest familiality scores of all cancers as measured by case-control studies, yet only a handful of genes have been implicated until now. Variants in microRNAs have been associated with the risk of several cancers including PTC but the magnitude of this involvement is unclear. This study was designed to test to what extent genomic variants in microRNAs contribute to PTC risk. We used SOLiD technology to sequence 321 genomic regions encoding 427 miRNAs in one affected individual from each of 80 PTC families. After excluding variants with frequency ≥ 1% in 1000 Genomes Phase 1 (n = 1092) we detected 1978 variants. After further functional filtering steps 25 variants in pre-miRs remained. Co-segregation was observed for six out of 16 tested miRNA variants with PTC in the families, namely let-7e, miR-181b, miR-135a, miR-15b, miR-320, and miR-484. Expression of miR-135a and miR-181b was tested in normal thyroid and tumor tissue from patients that carry the variants and a decrease in expression was observed. In vitro assays were applied to measure the effect of the variants on microRNAs' maturation. Four out of six variants were tested. Only the let-7e and miR-181b variants showed an effect on processing leading to lower levels of mature miRNA. These two variants were not detected in 1170 sporadic PTC cases nor in 1404 controls. Taken together, our data show that high penetrance germline sequence variants of miRNAs potentially predispose to a fraction of all PTC but are not common.

  14. Influence of structure disorders and temperaturesof systems on the bio-energy transport in proteinmolecules (Ⅱ)

    Institute of Scientific and Technical Information of China (English)

    Xiao-feng PANG

    2008-01-01

    The influence of molecular structure disor-ders and physiological temperature on the states and properties of solitons as transporters of bio-energy are numerically studied through the fourth-order Runge-Kutta method and a new theory based on my paper [Front. Phys. China, 2007, 2(4): 469]. The structure dis-orders include fluctuations in the characteristic parame-ters of the spring constant, dipole-dipole interaction con-stant and exciton-phonon coupling constant, as well as the chain-chain interaction coefficient among the three channels and ground state energy resulting from the dis-order distributions of masses of amino acid residues and impurities. In this paper, we investigate the behav-iors and states of solitons in a single protein molecu-lar chain, and in α-Helix protein molecules with three channels. In the former we prove first that the new soli-tons can move without dispersion, retaining its shape, velocity and energy in a uniform and periodic protein molecule. In this case of structure disorder, the fluctu-ations of the spring constant, dipole-dipole interaction constant and exciton-phonon coupling constant, as well as the ground state energy and the disorder distribu-tions of masses of amino acid residues of the proteins influence the states and properties of motion of solitons. However, they are still quite stable and are very robust against these structure disorders, even in the presence of larger disorders in the sequence of masses, spring con-stants and coupling constants. Still, the solitons may disperse or be destroyed when the disorder distribution of the masses and fluctuations of structure parameters are quite great. If the effect of thermal perturbation of the environment on the soliton in nonuniform proteins is considered again, it is still thermally stable at the biolog-ical temperature of 300 K, and at the longer time period of 300 ps and larger spacing of 400 amino acids. The new soliton is also thermally stable in the case of motion

  15. Quo vadis hrvatsko športsko pravo (posebno na primjeru nacionalnog športskog saveza u stečaju i pretvorbe športskih objekata

    Directory of Open Access Journals (Sweden)

    Hrvoje Kačer

    2011-12-01

    Full Text Available Športsko pravo je dosta mlada pravna grana, što po naravi stvari znači i opasnost od određenih nedostataka kojih nema u tradicionalnim pravnim granama kao što je npr. građansko ili kazneno pravo. Obzirom na pravno načelo koje kao trajno vrijedi u pravu općenito lex specialis derogat legi generali relativno je lako kada ta posebna (športska u ovom slučaju pravna norma postoji. Međutim, kada se u jednom tako naglašeno posebnom području (prepunom specifičnosti kao što je šport dogodi da posebne pravne norme nema i kada je nužno primijeniti onu pravnu normu kod čijeg donositelja zasigurno u razmišljanju nije bilo športa, nastaje problem, ponekad čak i prava pravna katastrofa. Drukčije sigurno nije moguće ocijeniti situaciju postojanja normative koja spriječava osnivanje drugog nacionalnog saveza dok postoji onaj nad kojim je otvoren stečaj, a još više kada dođe do otkupa tražbina prema stečajnom dužniku i na taj način preuzimanja stečajnog dužnika sa svim pravima (uključujući i onaj članstva u Hrvatskom olimpijskom odboru kao da se radi o stečaju neke tvornice npr. Jedini izlaz je u donošenju posebne, izvorno športske specijalističke pravne norme, ako je ikako moguće čak i uz tzv. povratni ili retroaktivni učinak te norme. Neposredan povod ovom tekstu su događanja u hrvatskom športu na način da su dva hrvatska nacionalna športska saveza (odbojka i golf doživjela stečajni postupak i primjenu onih pravnih normi (jer drugih nema koja vrijede npr. za stečaj trgovačkih društava.Nažalost, reakcija hrvatskog zakonodavca na način koji je bio za očekivati (ali i zahtijevati je izostala i otvorila (još jednom ustavnopravno pitanje da li i što učiniti (unutar sustava trodiobe vlasti i kako sankcionirati (osim rezultatom na izborima nedopustivu pasivnost zakonodavca koja se na primjeru športskog prava dogodila već više puta, a kao stalno obilježje u pravu općenito je postojeća i dogodila se i doga

  16. Commercial-Scale Demonstration of the Liquid Phase Methanol (LPMEOH) Process

    Energy Technology Data Exchange (ETDEWEB)

    None

    1998-12-21

    The Liquid Phase Methanol (LPMEOW) Demonstration Project at Kingsport Tennessee, is a $213.7 million cooperative agreement between the U.S. Department of Energy (DOE) and Air Products Liquid Phase Conversion Company, L.P. (the Partnership) to produce methanol from coal-derived synthesis gas (syngas). Air Products and Chemicals, Inc. (Air Products) and Eastman Chemical Company (Eastman) formed the Partnership to execute the Demonstration Project. The LPMEOW Process Demonstration Unit was built at a site located at the Eastman complex in Kingsport. During this quarter, initial planning and procurement work began on the seven project sites which have been accepted for participation in the off-site, methanol product-use test plan. Two of the projects have begun pre-testing of equipment and three other projects have commenced with equipment procurement, Methanol produced from carbon monoxide (CO)- rich syngas at the Alternative Fuels Development Unit (AFDU) in LaPorte, TX has been shipped to four of the project sites in anticipation of the start of testing during the first quarter of calendar year 1998. Catalyst activity, as defined by the ratio of the rate constant at any point in time to the rate constant for a freshly reduced catalyst (as determined in the laboratory autoclave), continued to decline more rapidly than expected. In response to concentrations of arsenic and sulfbr detected on catalyst samples from the LPMEOW Reactor, Eastman replaced both the arsine- and sulfiwremoval material in the Eastman guard bed which treats the primary syngas feed stream (&danced Gas) prior to its introduction into both the Eastman fixed-bed methanol plant and the LPMEOWM Demonstration Unit. After restarting the demonstration unit, the catalyst deactivation rate remained essentially unchanged. Parallel testing in the laboratory using arsine-doped, and subsequently arsine- and SuIfi-doped syngas, ako ftiIed to prove that arsine was responsible for the higher-than-expected rate of

  17. ETIKA I POLITIKA NANOTEHNOLOGIJE

    Directory of Open Access Journals (Sweden)

    Luciano Delbianco

    2013-07-01

    Full Text Available Nanotehnologija bi mogla postati najutjecajniji čimbenik koji je preuzeo tehnološku industriju još od pojave Interneta. Nanotehnologija može povećati brzinu memorijskih čipova, ukloniti čestice zagađivača iz vode i zraka i brže pronaći stanice raka. Nanotehnologija nam može izmaći kontroli i prouzročiti kraj našeg postojanja kao ljudskih bića. Nanotehnologija može ublažiti glad u svijetu, očistiti okoliš, izliječiti rak, zajamčiti biblijski raspon životnog vijeka ili stvoriti neizrecivo užasna superoružja. Nanotehnologija bi mogla biti novi azbest. Nanotehnologija može potaknuti ekonomski razvoj kroz širenje istraživanja. Nanotehnologija može naštetiti mogućnostima siromašnih u zemljama u razvoju. Nanotehnologija može ujednačiti veličine molekule sladoleda. Nanotehnologija može omogućiti digitalnom fotoaparatu da radi u mraku. Nanotehnologija može očistiti otrovni otpad na atomskoj razini. Nanotehnologija može temeljito promijeniti svijet. Nanotehnologija može postati instrument terorizma. Nanotehnologija može dovesti do sljedeće industrijske revolucije. Nanotehnologija može preobraziti industriju hrane. Nanotehnologija može popraviti ozonski omotač. Nanotehnologija može promijeniti sve.Sve su to naslovi koji započinju s «Nanotehnologija može…» Što bismo mi trebali zaključiti na temelju ovih nevjerojatno proturječnih obećanja i upozorenja? Kako jedna stvar može u sebi sadržavati toliko potencijala, čak i ako uzmemo u obzir hiperbolički entuzijazam stručnjaka za odnose s javnošću i novinara? Unatoč tim divljim obećanjima, postoji nešto što je specifično za nanotehnologiju, i postoji nekoliko vrlo specifičnih pitanja kojima bi se trebali baviti građani, političari, znanstvenici i poslovni ljudi zainteresirani za ovo područje. Da bismo procijenili etičke, pravne i političke aspekte nanotehnologije, ključno je razdvojiti potencijal nanotehnologije kojeg je moguće pratiti od

  18. Neki problemi pri prevođenju moderne poezije promatrani kroz teoriju konceptualne integracije

    Directory of Open Access Journals (Sweden)

    Sanja Cimer

    2011-12-01

    koji slijede u vlastitom su prijevodu, osim ako nije drugačije naznačeno. U 20. stoljeću oformio se, između ostalih, pristup da je zadatak prevoditelja prijevodom oponašati izvorno lirsko djelo, a uz to što prijevod treba preslikavati original, on također treba zadržati i njegovu poetsku vrijednost te u ciljnom jeziku funkcionirati kao samostalna poetska forma (bez glosa, komentara ili fusnota. Budući da je svaka pjesma priča za sebe, svaki je prevoditelj pri definiranju prioriteta (zvučnost? semantika? rima? ritam? prepušten samom sebi i konstantno se suočava s izborima (Jones (citat u Connolly 174 prevođenje poezije naziva “umjetnošću kompromisa”. W. S. Merwin opisuje svoj pristup prevođenju poezije na sljedeći način: “Znate, i dalje vjerujem da ne znam kako se prevodi i da to nitko ne zna. To je nemoguć, ali nužan proces, ne postoji savršen način da se on izvede i velik njegov dio mora se oblikovati u hodu za svaku pojedinačnu pjesmu.”

  19. Hymenobacter perfusus sp. nov., Hymenobacter flocculans sp. nov. and Hymenobacter metalli sp. nov. three new species isolated from an uranium mine waste water treatment system.

    Science.gov (United States)

    Chung, Ana Paula; Lopes, André; Nobre, M Fernanda; Morais, Paula V

    2010-12-01

    Three red-pink pigmented strains, designated A1-12(T), A2-50A(T) and A2-91(T), were recovered from two different sites in a uranium mine. For all strains, the optimum growth temperature was 25°C, the optimum pH was 6.0-6.5 and the DNA G+C contents were between 60 and 63.4 mol%. The major respiratory quinone was menaquinone 7 (MK-7) and the fatty acid profiles contained iso- and anteiso-branched C15 fatty acids, summed feature 3 (16:1 ω6c and/or ω7c and/or 15:0 iso 2-OH), summed feature 4 (17:1 anteiso B and/or iso I) and the unsaturated fatty acid 16:1 ω5c as the major components. Phylogenetic analysis of the 16S rRNA gene sequences showed that these organisms represented three distinct branches within the family Flexibacteraceae most closely related to the members of the genus Hymenobacter. Strain A1-12(T) formed a distinct phylogenetic line along with H. rigui KCTC 12533(T) and they shared approximately 98.9% 16S rRNA gene sequence similarity. However, these two strains shared only 14.7% pairwise similarity in their genomic DNA. Strains A2-50A(T) and A2-91(T) formed two distinct lineages, related to the species H. soli KCTC 12607(T), sharing about 95.5% 16S rRNA gene sequence similarity between themselves, and 88.3 and 92.0% with other members of the genus Hymenobacter. Based on the phylogenetic analysis and physiological and biochemical characteristics, these isolates were considered to represent three novel species for which we propose the names Hymenobacter perfusus for strain A1-12(T) (=CIP 110166=LMG 26000), Hymenobacter flocculans for strain A2-50A(T) (=CIP 110139=LMG 25699) and Hymenobacter metalli for strain A2-91(T) (=CIP 110140=LMG 25700).

  20. Ethical issues in human reproduction: Protestant perspectives in the light of European Protestant and Reformed Churches.

    Science.gov (United States)

    Birkhäuser, Martin

    2013-11-01

    Protestantism is not a centralized religion. It is composed by many independent Churches having different moral and ethical standards. This review concentrates on the ethical principles prevalent in most modern European Reformed Churches. It does not intend to discuss the ethical principles of many other Protestant Churches present mainly in the USA. The common foundations of Protestant theology are the "five sola ("Sola scriptura", Sola fide", "Sola gratia", Solus Christus or Solo Christo", "Soli deo gloria"). In opposition to the Catholic Church, no intermediary is needed between the Bible and the believer. As a consequence, Protestant Churches have no Magisterium, such as the Catholic Church. Therefore Protestant Churches cannot declare a certain position to be the "official position". Each Christian is personally responsible for all his acts, including his ethical behaviour. There is no complete unanimity among all Protestants on ethics or on any other issue. Human dignity, personal rights and self-determination have to be respected in each ethical consideration. The supersession of the Old Mosaic Covenant (including traditional Jewish law or Halakhah, maintained in Catholicism) by the New Covenant and by Christian Theology has an important impact on Protestant ethics in reproductive medicine. In the New Covenant, the Protestants Churches did not maintain the mandatory obligation from the old Mosaic Covenant to be fruitful and to multiply: there is no divine obligation by God to procreate. As a consequence, contraception is not a sin and not unethical. The status of the embryo is the key for the ethical consideration of all methods used in reproductive medicine. Most representatives of modern Protestant theology and bioethics defend the opinion that the embryo is not an independent human being as is the newborn child. For most Protestant bio-ethicists, as long as an embryo has no nervous system, no organs and no pain receptors, it cannot be seen as a human

  1. Comprehensive Assessments of RNA-seq by the SEQC Consortium: FDA-Led Efforts Advance Precision Medicine

    Directory of Open Access Journals (Sweden)

    Joshua Xu

    2016-03-01

    Full Text Available Studies on gene expression in response to therapy have led to the discovery of pharmacogenomics biomarkers and advances in precision medicine. Whole transcriptome sequencing (RNA-seq is an emerging tool for profiling gene expression and has received wide adoption in the biomedical research community. However, its value in regulatory decision making requires rigorous assessment and consensus between various stakeholders, including the research community, regulatory agencies, and industry. The FDA-led SEquencing Quality Control (SEQC consortium has made considerable progress in this direction, and is the subject of this review. Specifically, three RNA-seq platforms (Illumina HiSeq, Life Technologies SOLiD, and Roche 454 were extensively evaluated at multiple sites to assess cross-site and cross-platform reproducibility. The results demonstrated that relative gene expression measurements were consistently comparable across labs and platforms, but not so for the measurement of absolute expression levels. As part of the quality evaluation several studies were included to evaluate the utility of RNA-seq in clinical settings and safety assessment. The neuroblastoma study profiled tumor samples from 498 pediatric neuroblastoma patients by both microarray and RNA-seq. RNA-seq offers more utilities than microarray in determining the transcriptomic characteristics of cancer. However, RNA-seq and microarray-based models were comparable in clinical endpoint prediction, even when including additional features unique to RNA-seq beyond gene expression. The toxicogenomics study compared microarray and RNA-seq profiles of the liver samples from rats exposed to 27 different chemicals representing multiple toxicity modes of action. Cross-platform concordance was dependent on chemical treatment and transcript abundance. Though both RNA-seq and microarray are suitable for developing gene expression based predictive models with comparable prediction performance, RNA

  2. Intoxicaciones agudas en Santiago de Compostela,en un período de cuatro años

    Directory of Open Access Journals (Sweden)

    P. Fernández

    2003-01-01

    Full Text Available El objetivo de este trabajo fue evaluar las caracterís-ticas de un grupo de pacientes intoxicados en el área sanitaria deSantiago de Compostela. Para ello, se estudiaron 1192 intoxica-ciones agudas atendidas en el Servicio de Urgencias y Unidadde Cuidados Intensivos del Hospital Clínico Universitario deesta ciudad, desde el 1 de enero de 1993 al 31 de diciembre de1996. Se han considerado solo aquellos casos en los que se soli-citó un análisis toxicológico a este Instituto de Medicina Legal,desconociéndose el número de casos excluidos. Se diseñó unaficha clínica con todos los parámetros estudiados para su cum-plimentación por los médicos en el momento del ingreso. Seobservó un predominio del sexo masculino (61,4% y una edadmedia, en la población estudiada, de 32,3±12,5 años (rango: 15-93 años. Los tóxicos más frecuentemente implicados fueron lasdrogas de abuso (681 casos, seguidas de los fármacos (545casos y de “otros agentes” (88 casos. Las intoxicaciones vo-luntarias estaban asociadas mayoritariamente con mujeres y coningesta de fármacos, en contra de las accidentales, asociadas conhombres y con consumo de drogas de abuso. Existen anteceden-tes psiquiátricos en 332 casos, tratamiento farmacológico previoen 219 casos, alcoholismo en 189 casos y drogadicción por víaparenteral en 144 casos. Se ha constatado que un 76% de lospacientes presentaban alguna clínica al ingreso, con un predo-minio de las manifestaciones neurológicas (682 casos. Se utili-zó algún tipo de tratamiento específico en 574 sujetos, desta-cando la aplicación de lavado gástrico (444 casos y la adminis-tración de antídotos (358 casos. La mayoría de los pacientesevolucionó satisfactoriamente, con solo un 2% de mortalidad.Esta información permite conocer los patrones de abuso y la efi-cacia del tratamiento aplicado con el fin de mejorar el controldel paciente intoxicado

  3. Identification of microRNA genes in three opisthorchiids.

    Directory of Open Access Journals (Sweden)

    Vladimir Y Ovchinnikov

    2015-04-01

    Full Text Available Opisthorchis felineus, O. viverrini, and Clonorchis sinensis (family Opisthorchiidae are parasitic flatworms that pose a serious threat to humans in some countries and cause opisthorchiasis/clonorchiasis. Chronic disease may lead to a risk of carcinogenesis in the biliary ducts. MicroRNAs (miRNAs are small noncoding RNAs that control gene expression at post-transcriptional level and are implicated in the regulation of various cellular processes during the parasite- host interplay. However, to date, the miRNAs of opisthorchiid flukes, in particular those essential for maintaining their complex biology and parasitic mode of existence, have not been satisfactorily described.Using a SOLiD deep sequencing-bioinformatic approach, we identified 43 novel and 18 conserved miRNAs for O. felineus (miracidia, metacercariae and adult worms, 20 novel and 16 conserved miRNAs for O. viverrini (adult worms, and 33 novel and 18 conserved miRNAs for C. sinensis (adult worms. The analysis of the data revealed differences in the expression level of conserved miRNAs among the three species and among three the developmental stages of O. felineus. Analysis of miRNA genes revealed two gene clusters, one cluster-like region and one intronic miRNA in the genome. The presence and structure of the two gene clusters were validated using a PCR-based approach in the three flukes.This study represents a comprehensive description of miRNAs in three members of the family Opistorchiidae, significantly expands our knowledge of miRNAs in multicellular parasites and provides a basis for understanding the structural and functional evolution of miRNAs in these metazoan parasites. Results of this study also provides novel resources for deeper understanding the complex parasite biology, for further research on the pathogenesis and molecular events of disease induced by the liver flukes. The present data may also facilitate the development of novel approaches for the prevention and

  4. Students' Sex Mentality of One Medical College%某医学院本科生性心理现状分析

    Institute of Scientific and Technical Information of China (English)

    李志刚; 马长征

    2009-01-01

    目的 了解医学生性心理现状,为建立高校性心理健康教育模式提供依据.方法 采用自编问卷对某医学院399名本科医学生进行问卷调查.结果 男、女生是否喜欢自身性别的报告率差异有统计学意义(P<0.01),男生更愿意接受自身性别;大多数学生都希望与异性交往,且男生的需求更强烈;男、女生在释放性心理压力的途径与方法上差异有统计学意义(P<0.05);多数学生认为大学期间恋爱动机是排遣寂寞.结论 医学生性发展与性知识不协调,性需求和性满足之间存在冲突和矛盾.应科学引导学生树立正确的恋爱观,重视在校园内开展性知识教育和性文化建设.%Objective To understand medical students" current sex mentality, and to provide bases for establishing sex mental health edu-cation mode. Methods By using self-designed questionnaire, 399 medical students were investigated. Results There was statistical significant difference about the opinion towards whether they like their genders or not(P <0.01) , male students were more willing to accept their sex identities. Moat students were expecting to sooialize to socialize with the opposite genders and the male students manifested stronger desire. There were huge gen-der differences in the ways to releasing sex mental pressure (P < 0.05). Most students regarded love affaires in campus as a way of casting off soli-tude. Conclusion Medical college students" sex development and sex knowledge are not coordinated. There are conflicts between sex desire and fulfillment. It's advisable to help students develop proper ideas about love by sex education and spreading of its related culture.

  5. An assessment of the impact of local processes on dust lifting in martian climate models

    Science.gov (United States)

    Mulholland, David P.; Spiga, Aymeric; Listowski, Constantino; Read, Peter L.

    2015-05-01

    observed, including the flushing storm sources of Chryse and Utopia, and southern midlatitude areas from which larger storms tend to initiate, such as Hellas and Solis Planum. The seasonal cycle of emission, which includes a double-peaked structure in northern autumn and winter, also appears realistic. Significant increases to lifting rates are produced for any sensible choices of parameters controlling the sub-grid distributions used, but results are sensitive to the smallest scale of variability considered, which high-resolution modelling suggests should be O(1 km) or less. Use of such models in future will permit the use of a diagnosed (rather than prescribed) variable gustiness intensity, which should further enhance dust lifting in the southern hemisphere in particular.

  6. Educare educando all'opera: socialità e individualità nella 'Fanciulla del West' di Puccini

    Directory of Open Access Journals (Sweden)

    Andrea Chegai

    2014-12-01

    Full Text Available In svariate occasioni i musicologi hanno posto l’accento sull’efficacia educativa dell’opera per lo sviluppo della dimensione sentimentale di soggetti a diversi livelli scolari. Insegnare a cogliere la valenza drammatica di una scena o di un’aria e delle passioni che la animano tramite i contenuti del testo verbale e l’assetto musicale stabilito dal compositore è un ottimo viatico per educare all’ascolto della musica cogliendo le opportunità che essa offre a livello di formazione generale. A questo aspetto potrebbe essere utilmente affiancata la componente interrelazionale che costituisce la sostanza di cui si approvvigiona il teatro musicale di ogni epoca e tradizione. In quanto dramma, l’opera in musica inscena vicende fondate su relazioni interpersonali molteplici; sodalizi e dissidi, legami familiari e amorosi, passioni apparenti o tacite, aspirazioni legittime o illecite e ostacoli che si frappongono al loro conseguimento, socialità o viceversa solitudini ed isolamento. A ognuna di queste condizioni è nella finzione drammatica garantito diritto di esistenza. Persino la crudeltà o l’inganno possono mostrarsi seducenti, in un duetto, ma un ascolto competente riesce a darne conto e a smascherarli. La solitudine di un monologo procura all’opera di ogni tempo esiti musicalmente ragguardevoli, e anche in questo caso se ne può trarre un utile in termini di educazione affettiva (non c’è niente di sbagliato nel sentirsi soli e diversi dagli altri: è uno stato che serve alla crescita e può persino generare bellezza. Buoni e cattivi si confrontano, e come nella vita reale capita che tanto gli uni quanto gli altri possano avere la meglio: l’intreccio del dramma aiuterà a comprendere cosa eventualmente è andato storto; e come nella vita reale capita anche che buoni e cattivi si confondano fra loro. L’opera, contraddistinta dalla presentificazione specifica dei generi performativi, e anche per questo di forte presa

  7. Origins of a 350-kilobase genomic duplication in Mycobacterium tuberculosis and its impact on virulence.

    Science.gov (United States)

    Domenech, Pilar; Rog, Anya; Moolji, Jalal-ud-din; Radomski, Nicolas; Fallow, Ashley; Leon-Solis, Lizbel; Bowes, Julia; Behr, Marcel A; Reed, Michael B

    2014-07-01

    In the present study, we have investigated the evolution and impact on virulence of a 350-kb genomic duplication present in the most recently evolved members of the Mycobacterium tuberculosis East Asian lineage. In a mouse model of infection, comparing HN878 subclones HN878-27 (no duplication) and HN878-45 (with the 350-kb duplication) revealed that the latter is impaired for in vivo growth during the initial 3 weeks of infection. Furthermore, the median survival time of mice infected with isolate HN878-45 is significantly longer (77 days) than that of mice infected with HN878-27. Whole-genome sequencing of both isolates failed to reveal any mutational events other than the duplication that could account for such a substantial difference in virulence. Although we and others had previously speculated that the 350-kb duplication arose in response to some form of host-applied selective pressure (P. Domenech, G. S. Kolly, L. Leon-Solis, A. Fallow, M. B. Reed, J. Bacteriol. 192: 4562-4570, 2010, and B. Weiner, J. Gomez, T. C. Victor, R. M. Warren, A. Sloutsky, B. B. Plikaytis, J. E. Posey, P. D. van Helden, N. C. Gey van Pittius, M. Koehrsen, P. Sisk, C. Stolte, J. White, S. Gagneux, B. Birren, D. Hung, M. Murray, J. Galagan, PLoS One 7: e26038, 2012), here we show that this large chromosomal amplification event is very rapidly selected within standard in vitro broth cultures in a range of isolates. Indeed, subclones harboring the duplication were detectable after just five rounds of in vitro passage. In contrast, the duplication appears to be highly unstable in vivo and is negatively selected during the later stages of infection in mice. We believe that the rapid in vitro evolution of M. tuberculosis is an underappreciated aspect of its biology that is often ignored, despite the fact that it has the potential to confound the data and conclusions arising from comparative studies of isolates at both the genotypic and phenotypic levels.

  8. Identification of genetic risk variants for deep vein thrombosis by multiplexed next-generation sequencing of 186 hemostatic/pro-inflammatory genes

    Directory of Open Access Journals (Sweden)

    Lotta Luca A

    2012-02-01

    Full Text Available Abstract Background Next-generation DNA sequencing is opening new avenues for genetic association studies in common diseases that, like deep vein thrombosis (DVT, have a strong genetic predisposition still largely unexplained by currently identified risk variants. In order to develop sequencing and analytical pipelines for the application of next-generation sequencing to complex diseases, we conducted a pilot study sequencing the coding area of 186 hemostatic/proinflammatory genes in 10 Italian cases of idiopathic DVT and 12 healthy controls. Results A molecular-barcoding strategy was used to multiplex DNA target capture and sequencing, while retaining individual sequence information. Genomic libraries with barcode sequence-tags were pooled (in pools of 8 or 16 samples and enriched for target DNA sequences. Sequencing was performed on ABI SOLiD-4 platforms. We produced > 12 gigabases of raw sequence data to sequence at high coverage (average: 42X the 700-kilobase target area in 22 individuals. A total of 1876 high-quality genetic variants were identified (1778 single nucleotide substitutions and 98 insertions/deletions. Annotation on databases of genetic variation and human disease mutations revealed several novel, potentially deleterious mutations. We tested 576 common variants in a case-control association analysis, carrying the top-5 associations over to replication in up to 719 DVT cases and 719 controls. We also conducted an analysis of the burden of nonsynonymous variants in coagulation factor and anticoagulant genes. We found an excess of rare missense mutations in anticoagulant genes in DVT cases compared to controls and an association for a missense polymorphism of FGA (rs6050; p = 1.9 × 10-5, OR 1.45; 95% CI, 1.22-1.72; after replication in > 1400 individuals. Conclusions We implemented a barcode-based strategy to efficiently multiplex sequencing of hundreds of candidate genes in several individuals. In the relatively small dataset of

  9. Genome Project Standards in a New Era of Sequencing

    Energy Technology Data Exchange (ETDEWEB)

    GSC Consortia; HMP Jumpstart Consortia; Chain, P. S. G.; Grafham, D. V.; Fulton, R. S.; FitzGerald, M. G.; Hostetler, J.; Muzny, D.; Detter, J. C.; Ali, J.; Birren, B.; Bruce, D. C.; Buhay, C.; Cole, J. R.; Ding, Y.; Dugan, S.; Field, D.; Garrity, G. M.; Gibbs, R.; Graves, T.; Han, C. S.; Harrison, S. H.; Highlander, S.; Hugenholtz, P.; Khouri, H. M.; Kodira, C. D.; Kolker, E.; Kyrpides, N. C.; Lang, D.; Lapidus, A.; Malfatti, S. A.; Markowitz, V.; Metha, T.; Nelson, K. E.; Parkhill, J.; Pitluck, S.; Qin, X.; Read, T. D.; Schmutz, J.; Sozhamannan, S.; Strausberg, R.; Sutton, G.; Thomson, N. R.; Tiedje, J. M.; Weinstock, G.; Wollam, A.

    2009-06-01

    For over a decade, genome 43 sequences have adhered to only two standards that are relied on for purposes of sequence analysis by interested third parties (1, 2). However, ongoing developments in revolutionary sequencing technologies have resulted in a redefinition of traditional whole genome sequencing that requires a careful reevaluation of such standards. With commercially available 454 pyrosequencing (followed by Illumina, SOLiD, and now Helicos), there has been an explosion of genomes sequenced under the moniker 'draft', however these can be very poor quality genomes (due to inherent errors in the sequencing technologies, and the inability of assembly programs to fully address these errors). Further, one can only infer that such draft genomes may be of poor quality by navigating through the databases to find the number and type of reads deposited in sequence trace repositories (and not all genomes have this available), or to identify the number of contigs or genome fragments deposited to the database. The difficulty in assessing the quality of such deposited genomes has created some havoc for genome analysis pipelines and contributed to many wasted hours of (mis)interpretation. These same novel sequencing technologies have also brought an exponential leap in raw sequencing capability, and at greatly reduced prices that have further skewed the time- and cost-ratios of draft data generation versus the painstaking process of improving and finishing a genome. The resulting effect is an ever-widening gap between drafted and finished genomes that only promises to continue (Figure 1), hence there is an urgent need to distinguish good and poor datasets. The sequencing institutes in the authorship, along with the NIH's Human Microbiome Project Jumpstart Consortium (3), strongly believe that a new set of standards is required for genome sequences. The following represents a set of six community-defined categories of genome sequence standards that better

  10. Unusual Polar Activity of the Sun in the Northern Hemisphere and Its Implications for Solar Cycle 25

    Science.gov (United States)

    Gopalswamy, Nat; Masuda, Satoshi; Yashiro, Seiji; Akiyama, Sachiko; Shibasaki, Kiyoto

    2016-07-01

    Polar field strength in one solar cycle is known to indicate the strength (e.g., Sunspot number) and phase of the next cycle. In particular the polar field strength (or its proxies such as the polar coronal hole area and microwave polar brightness) during the minimum phase of a given cycle seem to be well correlated with the maximum sunspot number of the next cycle. Polar prominence eruptions and coronal mass ejections have also been found to be indicators of low polar field; their cessation signals the time of polarity reversal. While these indicators are present in the current cycle, significant differences are found regarding the phase lag between the two hemispheres and the duration of polar eruptions. We use data from the Nobeyama Radioheliograph, the Solar Dynamics Observatory, SOLIS, and Wilcox Solar Observatory to highlight these differences. We find that the north polar region of the Sun has near-zero field strength for more than three years. This is unusually long and caused by surges of both polarities heading toward the north pole that prevent the buildup of the polar field. This seems to be due to anti-Hale active regions that appeared around the 2012 peak sunspot activity in the northern hemisphere. The unusual condition is consistent with (i) the continued high-latitude prominence eruption, (ii) the extended period of high tilt angle of the heliospheric current sheet, (iii) the weak microwave polar brightness, and (iv) the lack of north polar coronal hole. On the other hand, the south polar field has started building up and the coronal hole has appeared in early 2015 because of large active regions of the correct tilt in the southern hemisphere during the 2014 peak of sunspot activity. The extended period of near-zero field in the north polar region should result in very weak and delayed sunspot activity in the northern hemisphere in cycle 25. On the other hand the south polar field has already increased significantly, suggesting that the activity in

  11. Comparison of the bacterial species diversity of spontaneous cocoa bean fermentations carried out at selected farms in Ivory Coast and Brazil.

    Science.gov (United States)

    Papalexandratou, Zoi; Camu, Nicholas; Falony, Gwen; De Vuyst, Luc

    2011-08-01

    To compare the spontaneous cocoa bean fermentation process carried out in different cocoa-producing regions, heap and box (one Ivorian farm) and box (two Brazilian farms) fermentations were carried out. All fermentations were studied through a multiphasic approach. In general, the temperature inside the fermenting mass increased throughout all fermentations and reached end-values of 42-48 °C. The main end-products of pulp carbohydrate catabolism were ethanol, lactic acid, acetic acid, and/or mannitol. In the case of the fermentations on the selected Ivorian farm, the species diversity of lactic acid bacteria (LAB) and acetic acid bacteria (AAB) was restricted. Lactobacillus fermentum and Leuconostoc pseudomesenteroides were the predominant LAB species, due to their ethanol and acid tolerance and citrate consumption. The levels of mannitol, ascribed to growth of L. fermentum, were fermentation-dependent. Also, enterobacterial species, such as Erwinia soli and Pantoea sp., were among the predominating microbiota during the early stages of both heap and box fermentations in Ivory Coast, which could be responsible for gluconic acid production. Consumption of gluconic acid at the initial phases of the Ivorian fermentations could be due to yeast growth. A wider microbial species diversity throughout the fermentation process was seen in the case of the box fermentations on the selected Brazilian farms, which differed, amongst other factors, regarding pod/bean selection on these farms as compared to fermentations on the selected Ivorian farm. This microbiota included Lactobacillus plantarum, Lactobacillus durianis, L. fermentum, Lactobacillus mali, Lactobacillus nagelii, L. pseudomesenteroides, and Pediococcus acidilactici, as well as Bacillus subtilis that was present at late fermentation, when the temperature inside the fermenting mass reached values higher than 50 °C. Moreover, AAB seemed to dominate the Brazilian box fermentations studied, explaining higher acetic

  12. Surface-compositional Properties of Lava Plains in Syria-Thaumasia Block, Mars

    Science.gov (United States)

    Huang, J.; Xiao, L.; Kraft, M. D.; Christensen, P. R.; Edwards, C. S.; Ruff, S. W.; Dohm, J.

    2012-12-01

    Mars has a long and complex volcanic history (Greeley and Spudis, 1981; Carr, 2006). Among abundant plain-style volcanism and various edifices, Tharsis bulge is a prominent and long-lasting (Werner, 2009) volcanic province. However, there is little report about compositional variations before and after Tharsis uplift. The Syria- Thaumasia block (STB) is a complex tectono-volcanic province related to the Tharsis bulge. Understanding its formation is critical to characterizing the early history and planetary evolution of Mars. The STB lies at the southern edge of Tharsis bulge. It consists of lava plains (Syria, Solis, Sinai and Thaumasia Plana) bounded by an arcuate region of higher topography (Thaumasia Highlands, Melas Dorsa and Coprates Rise) and Valles Marineris to the north. Previous work on surface thermophysical properties (Christensen, 1988; Jakosky et al., 2000; Putzig and Mellon, 2007) and visible/near infrared and thermal infrared remote sensing spectroscopic compositional analysis (Bandfield, 2000; Bibring et al., 2006; Rogers and Christensen, 2007) had been done only in a global scale, but regional study of both surface thermophysical properties and compositions for each of the distinct lava plains in STB is lacking. In this study, we characterize a variety of volcanic features, including lava tubes, channels and their relationships with wrinkle ridges within lava plains using THEMIS infrared data (100 m/pixel: Christensen et al., 2004), CTX data (6 m/pixel: Malin et al., 2007) and HiRISE data (25 cm/pixel: McEwen et al., 2007). We assessed the surface thermophysical properties and compositions of lava plains using TES data (Christensen et al., 2001). The geomorphic features imply the lava emplacement mechanisms, while their relationships indicate the chronologic relationships between Tharsis uplift and lava emplacement. The compositional results show variations within the lava plains (Table 1), while the thermophysical results show the compositional

  13. Music and the Nature: Input of the Czech Composers

    Science.gov (United States)

    Nemec, Vaclav; Nemcova, Lidmila

    2014-05-01

    Slovak mountains. Josef Suk (1874 - 1935): Pohádka léta (A Summer's Tale) - a work for orchestra remembering a relax and consolation in beauties of the Nature. Bohuslav Martinů (1890 - 1959): Otvírání studánek (The Opening of the Springs) - cantata for soli, female chorus and instrumental accompaniment remembering old local customs of purifying water in the wells when spring comes.

  14. HIGH RESOLUTION SONOGRAPHIC EVALUATION OF PALPABLE THYROID LESIONS WITH HISTOPATHOLOGICAL CORRELATION- A PROSP ECTIVE STUDY

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    Malik

    2013-05-01

    Full Text Available ABSTRACT: Thyroid lesions are found in 3-7% of population wor ldwide. The overwhelming majority of thyroid nodules are benign in nature. Thyroid ca ncers are rare; however thyroid carcinoma is the most common malignancy involving the endocrine glan ds and is responsible for approximately 1.5% of new cancer cases annually. The clinical challeng e is to distinguish the few clinically significant malignant nodules from the many benign nodules beca use malignant thyroid lesions shows good long term prognosis after surgical excision. Aims : To Assess the sensitivity and specificity of sonographic findings in differentiation of benign a nd malignant thyroid lesion. Method: A hospital based prospective study was done on 100 patients wi th palpable thyroid nodules using high resolution ultrasonography and findings correlated with fine needle aspiration cytology. Result: Sensitivity and Specificity of sonography to differ entiate benign and malignant thyroid lesions (FNAC correlation was 70.59 % & 96.39 % respectively. Pr esence of non shadowing echogenic cholesterol crystals showed high sensitivity of 58.70 % and spe cificity of 83.33 % for benign thyroid nodules. Sensitivity and Specificity of absence of thin peri pheral halo around malignant lesions were 70.4 % & 65.8% respectively. Sensitivity and Specificity of hypoechogenicity for malignant lesions were 64.71 % and 98.80 %.Presence of fine calcification was sh owing high Sensitivity & Specificity of 64.71 % and 93.98 % respectively in malignant lesions. Pred ominant intra-nodular colour flow pattern in solid nodules was predictive of malignancy with Sensitivi ty 82.35 and Specificity 84.34 %. Higher RI value (greater than 0.77 were significantly found in mal ignant lesions with sensitivity of 78.57 % and specificity of 92.31%. Solid and predominantly soli d lesions show high Sensitivity, Specificity for being a malignant lesion 70.59 % & 55.42 respective ly but less Positive Predictive Value of24

  15. High throughput sequencing in mice: a platform comparison identifies a preponderance of cryptic SNPs

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    Darakjian Priscila

    2009-08-01

    Full Text Available Abstract Background Allelic variation is the cornerstone of genetically determined differences in gene expression, gene product structure, physiology, and behavior. However, allelic variation, particularly cryptic (unknown or not annotated variation, is problematic for follow up analyses. Polymorphisms result in a high incidence of false positive and false negative results in hybridization based analyses and hinder the identification of the true variation underlying genetically determined differences in physiology and behavior. Given the proliferation of mouse genetic models (e.g., knockout models, selectively bred lines, heterogeneous stocks derived from standard inbred strains and wild mice and the wealth of gene expression microarray and phenotypic studies using genetic models, the impact of naturally-occurring polymorphisms on these data is critical. With the advent of next-generation, high-throughput sequencing, we are now in a position to determine to what extent polymorphisms are currently cryptic in such models and their impact on downstream analyses. Results We sequenced the two most commonly used inbred mouse strains, DBA/2J and C57BL/6J, across a region of chromosome 1 (171.6 – 174.6 megabases using two next generation high-throughput sequencing platforms: Applied Biosystems (SOLiD and Illumina (Genome Analyzer. Using the same templates on both platforms, we compared realignments and single nucleotide polymorphism (SNP detection with an 80 fold average read depth across platforms and samples. While public datasets currently annotate 4,527 SNPs between the two strains in this interval, thorough high-throughput sequencing identified a total of 11,824 SNPs in the interval, including 7,663 new SNPs. Furthermore, we confirmed 40 missense SNPs and discovered 36 new missense SNPs. Conclusion Comparisons utilizing even two of the best characterized mouse genetic models, DBA/2J and C57BL/6J, indicate that more than half of naturally

  16. Adaptedd Winkler model based on nonlinear dynamic analysis of soil-pile%基于桩-土非线性动力分析改进的Winkler模型

    Institute of Scientific and Technical Information of China (English)

    侯元恒

    2012-01-01

    一种数值模型用来研究地震作用下桩基础反应,在Winkler模型基础上,考虑了桩的柔性与能量耗散及辐射.模型用来研究桩-土-结构共同作用分析,包括桩土非线性效应,考虑桩土体系非线性及其在强震作用下会产生大的变形,桩采用材料与几何非线性的梁-柱元相同,而土体假想为沿桩长段均匀的非线性弹簧-阻尼器,进而采用修正的Ramberg-Osgood模型.利用剪切模量与阻尼一个关系导得土的阻尼.为了模拟土中辐射阻尼效应,桩与土采用粘性阻尼器连接.模型同文献中的振动台测试对比表明,该模型与测试结果较吻合,进而为强震作用下桩土分析提供一种数值分析方法.%A numerical model to study the seismic response of pile foundation,it is based on Winkler model,takes into account both pile flexibility and energy dissipation and radiation. The model is applied to study the influence of soil-pile-structure interaction on the response of structures,including nonlinear pile-soil effects. To incorporate nonlinearities in the soil-pile system might arise from large deformations under severe earthquake loading,the piles' material and geometry using non-linear the beam-column element, while the soil is idealized by nonlinear springs and dashpots uniformly attached to the piles along their length. For the soli springs, a modified Ramberg-Osgood model is employed;for the material damping of the soil,a simple relationship between the shear modulus and damping is utilized. To simulate the radiation damping effect in the soil, viscous dampers are attached to the pile. Model is compared with the literature of shaking table tests,indicating that the model agrees with the test results,and provides a numerical analysis for the strong motion of pile-soil analysis methods.

  17. Experimental study on soil moisture content of geogrid-reinforced clay under freezing-thawing cycle%冻融循环下格栅加筋粘土土体含水率试验研究

    Institute of Scientific and Technical Information of China (English)

    赵荣飞; 宓永宁; 姜广田; 金丹; 高微

    2012-01-01

    通过一系列室内含水率测试试验,研究了格栅加筋粘土在多次冻融循环条件下土体含水率的变动情况,探讨了填料压实度,加筋层数和初始含水率对冻融后土体含水率变动的影响规律.结果表明,压实度对土体ωi影响最大,低压实度时,△ω1为正值,高压实度时,△ω1为负值;加筋层数次之,ω1值随加筋层数增加而减小,在压实度高的土体中尤为显著;初始含水率影响最弱,仅在土体压实度较低时,较大的初始含水率会引起较大的△ω1值.%Through a series of lab-tests on moisture content of soil, the change of the moisture content of the grid-reinforced clay under freezing-thawing cycle is studied herein, and then the law of the impact from the compaction degree, the reinforcement layers and the initial moisture content of the soil on the moisture content of the soil under freezing-thawing cycles are discussed. The result shows that the compaction degree has the greatest impact on the soil body ωi, i. e. △ω1 is positive when the compaction degree is low and △ω1, is negative when the compaction degree is high; meanwhile, the number of the reinforcement layers only has a secondary impact on the soli body, i. e. the value of ω1, is decreased along with the increase of the number of the reinforcement layers, while it is significant in the highly compacted soil; moreover, the impact from the initial moisture content is much less and the greater value of △ω1 is to be only arisen from a greater initial moisture content when the compaction degree of soil is low.

  18. Crecimiento y supervivencia de crías de pulpo (Octopus maya : alimentados con dietas inertes bajo condiciones de laboratorio en Yucatán, México Growth and survival of larvals of octopus (Octopus maya : fed with inert diets under conditions of laboratory in Yucatan, Mexico

    Directory of Open Access Journals (Sweden)

    Miguel Rodríguez-Serna

    2008-12-01

    Full Text Available Bajo un diseño experimental se probaron tres dietas con el objetivo de determinar el crecimiento y la supervivencia de las crías de Octopus maya (Voss y Solís 1966, utilizando dos tipos de alimento inerte (A2 = Balanceado, A3 = Surimi y comparándolo con un alimento vivo (A1 = Artemia, bajo condiciones controladas. Los resultados mostraron diferencias significativas entre el alimento vivo y el alimento inerte. Se encontró un mejor crecimiento en peso ganado en las crías de pulpo alimentadas con una dieta viva en comparación con las dietas inertes (A1 = 11.88 g, A2 = 8.9 g, A3 = 8.83 g; sin embargo, para las tres dietas se obtuvo una baja supervivencia. El análisis estadístico arrojó diferencias estadísticas (p > 0.05 entre todos los tratamientos, siendo el alimento vivo el que presentó la mayor supervivencia con un 61.3%, seguido del surimi con un 42.3% y finalmente el balanceado con un 27.6%. La alimentación de O. maya en cautiverio aún requiere de muchos esfuerzos para desarrollar un alimento balanceado adecuado desde el punto de vista nutrimental o de formulación, así como desde la parte de atracción y aceptación por los animales.Three experimental diets were probed for the growth and survival rates of young Octopus maya (Voss and Solis 1966, using two inert foods (A2 = shrimp pellets, A3 = Surimi and one alive food (A1 = Artemia, under controlled conditions. The results showed significant differences between the live food and the inert ones. Young octopus feed with artemia, reported better growth in weight gained compared with the inert diets (A1 = 11.88 g, A2 = 8.9 g, A3 = 8.83 g. All three diets obtained low survivorships, being the alive food the best one with a rate of 61.3%, followed by surimi with 42.3%, and finally the pellet food with 27.6%. Statistical analysis showed differences (p > 0.05 between all the treatments for both growth and survival results. Artificial feeding of O. maya in captivity still requires many

  19. Whole genome resequencing of black Angus and Holstein cattle for SNP and CNV discovery

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    Stothard Paul

    2011-11-01

    Full Text Available Abstract Background One of the goals of livestock genomics research is to identify the genetic differences responsible for variation in phenotypic traits, particularly those of economic importance. Characterizing the genetic variation in livestock species is an important step towards linking genes or genomic regions with phenotypes. The completion of the bovine genome sequence and recent advances in DNA sequencing technology allow for in-depth characterization of the genetic variations present in cattle. Here we describe the whole-genome resequencing of two Bos taurus bulls from distinct breeds for the purpose of identifying and annotating novel forms of genetic variation in cattle. Results The genomes of a Black Angus bull and a Holstein bull were sequenced to 22-fold and 19-fold coverage, respectively, using the ABI SOLiD system. Comparisons of the sequences with the Btau4.0 reference assembly yielded 7 million single nucleotide polymorphisms (SNPs, 24% of which were identified in both animals. Of the total SNPs found in Holstein, Black Angus, and in both animals, 81%, 81%, and 75% respectively are novel. In-depth annotations of the data identified more than 16 thousand distinct non-synonymous SNPs (85% novel between the two datasets. Alignments between the SNP-altered proteins and orthologues from numerous species indicate that many of the SNPs alter well-conserved amino acids. Several SNPs predicted to create or remove stop codons were also found. A comparison between the sequencing SNPs and genotyping results from the BovineHD high-density genotyping chip indicates a detection rate of 91% for homozygous SNPs and 81% for heterozygous SNPs. The false positive rate is estimated to be about 2% for both the Black Angus and Holstein SNP sets, based on follow-up genotyping of 422 and 427 SNPs, respectively. Comparisons of read depth between the two bulls along the reference assembly identified 790 putative copy-number variations (CNVs. Ten

  20. An evaluation of alternative methods for constructing phylogenies from whole genome sequence data: a case study with Salmonella

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    James B. Pettengill

    2014-10-01

    Full Text Available Comparative genomics based on whole genome sequencing (WGS is increasingly being applied to investigate questions within evolutionary and molecular biology, as well as questions concerning public health (e.g., pathogen outbreaks. Given the impact that conclusions derived from such analyses may have, we have evaluated the robustness of clustering individuals based on WGS data to three key factors: (1 next-generation sequencing (NGS platform (HiSeq, MiSeq, IonTorrent, 454, and SOLiD, (2 algorithms used to construct a SNP (single nucleotide polymorphism matrix (reference-based and reference-free, and (3 phylogenetic inference method (FastTreeMP, GARLI, and RAxML. We carried out these analyses on 194 whole genome sequences representing 107 unique Salmonella enterica subsp. enterica ser. Montevideo strains. Reference-based approaches for identifying SNPs produced trees that were significantly more similar to one another than those produced under the reference-free approach. Topologies inferred using a core matrix (i.e., no missing data were significantly more discordant than those inferred using a non-core matrix that allows for some missing data. However, allowing for too much missing data likely results in a high false discovery rate of SNPs. When analyzing the same SNP matrix, we observed that the more thorough inference methods implemented in GARLI and RAxML produced more similar topologies than FastTreeMP. Our results also confirm that reproducibility varies among NGS platforms where the MiSeq had the lowest number of pairwise differences among replicate runs. Our investigation into the robustness of clustering patterns illustrates the importance of carefully considering how data from different platforms are combined and analyzed. We found clear differences in the topologies inferred, and certain methods performed significantly better than others for discriminating between the highly clonal organisms investigated here. The methods supported by

  1. Metagenomic Analysis of the Dynamic Changes in the Gut Microbiome of the Participants of the MARS-500 Experiment, Simulating Long Term Space Flight.

    Science.gov (United States)

    Mardanov, A V; Babykin, M M; Beletsky, A V; Grigoriev, A I; Zinchenko, V V; Kadnikov, V V; Kirpichnikov, M P; Mazur, A M; Nedoluzhko, A V; Novikova, N D; Prokhortchouk, E B; Ravin, N V; Skryabin, K G; Shestakov, S V

    2013-07-01

    A metagenomic analysis of the dynamic changes of the composition of the intestinal microbiome of five participants of the MARS-500 experiment was performed. DNA samples were isolated from the feces of the participants taken just before the experiment, upon 14, 30, 210, 363 and 510 days of isolation in the experimental module, and two weeks upon completion of the experiment. The taxonomic composition of the microbiome was analyzed by pyrosequencing of 16S rRNA gene fragments. Both the taxonomic and functional gene content of the microbiome of one participant were analyzed by whole metagenome sequencing using the SOLiD technique. Each participant had a specific microbiome that could be assigned to one of three recognized enterotypes. Two participants had enterotype I microbiomes characterized by the prevalence of Bacteroides, while the microbiomes of two others, assigned to type II, were dominated by Prevotella. One participant had a microbiome of mixed type. It was found that (1) changes in the taxonimic composition of the microbiomes occurred in the course of the experiment, but the enterotypes remained the same; (2) significant changes in the compositions of the microbiomes occurred just 14-30 days after the beginning of the experiment, presumably indicating the influence of stress factors in the first stage of the experiment; (3) a tendency toward a reversion of the microbiomes to their initial composition was observed two weeks after the end of the experiment, but complete recovery was not achieved. The metagenomic analysis of the microbiome of one of the participants showed that in spite of variations in the taxonomic compositions of microbiomes, the "functional" genetic composition was much more stable for most of the functional gene categories. Probably in the course of the experiment the taxonomic composition of the gut microbiome was adaptively changed to reflect the individual response to the experimental conditions. A new, balanced taxonomic composition

  2. Characterization of a biogas-producing microbial community by short-read next generation DNA sequencing

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    Wirth Roland

    2012-07-01

    Full Text Available Abstract Background Renewable energy production is currently a major issue worldwide. Biogas is a promising renewable energy carrier as the technology of its production combines the elimination of organic waste with the formation of a versatile energy carrier, methane. In consequence of the complexity of the microbial communities and metabolic pathways involved the biotechnology of the microbiological process leading to biogas production is poorly understood. Metagenomic approaches are suitable means of addressing related questions. In the present work a novel high-throughput technique was tested for its benefits in resolving the functional and taxonomical complexity of such microbial consortia. Results It was demonstrated that the extremely parallel SOLiD™ short-read DNA sequencing platform is capable of providing sufficient useful information to decipher the systematic and functional contexts within a biogas-producing community. Although this technology has not been employed to address such problems previously, the data obtained compare well with those from similar high-throughput approaches such as 454-pyrosequencing GS FLX or Titanium. The predominant microbes contributing to the decomposition of organic matter include members of the Eubacteria, class Clostridia, order Clostridiales, family Clostridiaceae. Bacteria belonging in other systematic groups contribute to the diversity of the microbial consortium. Archaea comprise a remarkably small minority in this community, given their crucial role in biogas production. Among the Archaea, the predominant order is the Methanomicrobiales and the most abundant species is Methanoculleus marisnigri. The Methanomicrobiales are hydrogenotrophic methanogens. Besides corroborating earlier findings on the significance of the contribution of the Clostridia to organic substrate decomposition, the results demonstrate the importance of the metabolism of hydrogen within the biogas producing microbial

  3. Il contributo di una Unitá Operativa Complessa: di Infettivologia Pediatrica durante l'epidemia di morbillo in Campania.

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    G. De Lorenzo

    2003-05-01

    Full Text Available

    Il morbillo è un importante problema di Sanità Pubblica che comporta un elevato rischio di complicanze con conseguenti eccessi di ospedalizzazione, mortalità e costi elevati.

    Obiettivi: Valutare incidenza, decorso clinico, complicanze e scelte terapeutiche in bambini ospedalizzati per morbillo, nonché l’impatto dell’epidemia su strutture ed operatori di una U.O.C. di Infettivologia Pediatrica con 16 p.l.

    Metodologia: dalle cartelle cliniche dei bambini ricoverati per morbillo presso l’U.O.C. di Pediatria Infettivologica dell’Ospedale Pausillipon di Napoli, nel corso dell’epidemia verificatasi in Campania tra gennaio e maggio 2002, sono stati raccolti, mediante una apposita griglia di rilevazione, i dati socio anagrafici, quelli relativi a decorso della malattia, eventuali complicanze, scelte terapeutiche adottate, stato immunitario del bambino e della madre, possibile fonte di contagio, motivazione al ricovero. Su 78 bambini ricoverati (38F e 40M, età media di 4,2 anni (range 0,1 14 anni, di cui 20 con età inferiore a 15 mesi, 42 hanno presentato complicanze: 27 broncopolmoniti, di cui 4 di presumibile origine virale e 23 da presumibile sovrainfezione batterica, 7 laringotracheobronchiti (croup, 4 diarrea con disidratazione, 1 otite media, 4 piastrinopenia transitoria, 2 stomatiti, 1 cistite emorragica, 1 meningoencefalite. Oltre alla terapia antibiotica e cortisonica (quando indicate, 27 bambini hanno ricevuto una singola somministrazione di Vitamina A, con decorso favorevole della malattia e nessun effetto collaterale. In soli due bambini era stata praticata la vaccinazione antimorbillo.

    Conclusioni: i dati confermano l’importanza della vaccinazione antimorbillo e l’opportunità di anticiparla a 6 mesi in corso di epidemia (nella quasi totalità dei casi di età ‹15 mesi la fonte di contagio è stata la malattia in

  4. Regulatory effects of adenosine A2A receptors on psychomotor ability and mood behavior of mice%腺苷A2A受体对小鼠精神运动和情绪行为的调控作用

    Institute of Scientific and Technical Information of China (English)

    姜莉; 冉鸿; 段炜; 郑健

    2011-01-01

    目的 探讨腺苷A2A受体基因敲除、A2A受体激动剂干预或A2A受体抑制剂干预对小鼠运动活性、焦虑和抑郁样行为的影响.方法 取腺苷A2A受体基因敲除(A2AKO)的雄性小鼠(A2AKO组)及同窝野生型(WT)C57BL/6小鼠(WT组)待用.另取雄性清洁级C57BL/6小鼠,随机分为SCH58261组、CGS21680组和对照组,分别给予腺苷A2A受体特异性拮抗剂SCH58261(2mg/kg)、腺苷A2A受体特异性激动剂CGS21680(0.5mg/kg)和同体积(0.25ml)载体溶液(二甲基亚砜+生理盐水)腹膜腔注射,10min后待用.前述各组别小鼠均进行旷场、高架十字迷宫和强迫游泳实验,测定各组小鼠的运动活性、焦虑和抑郁样行为.结果 与WT组比较,A2AKO组旷场总运动路程缩短(P<0.001),周边区域的滞留时间延长(P<0.05),进入高架十字迷宫开臂的次数及在开臂滞留时间减少(P<0.05),强迫游泳的累计不动时间无显著差异(P>0.05),而CGS21680组旷场总运动路程缩短(P<0.01),周边区域滞留时间延长(P<0.01),进入高架十字迷宫开臂的次数及在开臂的滞留时间减少(P<0.01),强迫游泳的累计不动时间延长(P<0.001),而SCH58261组旷场总运动路程和中心区域活动路程延长(P<0.001),强迫游泳的累计不动时间缩短(P<0.01),高架十字迷宫试验各项指标无显著差异(P>0.05).结论 腺苷A2A受体激动剂可减少小鼠的自发和探索行为,加重焦虑和抑郁情绪,该效应与腺苷A2A受体基因敲除引发的效应相似,与腺苷A2A受体拮抗剂引发效应相反.%Objective To explore the effects of gene knock-out, agonist or inhibitor of adenosine A2A receptor on the locomotor activity, and anxiety- or depression-like behavior of mice. Methods Male C57BL/6 mice, comprising those underwent gene knock-out of adenosine AjA receptor (AjaKO) and their wild-type (WT) littermates, were assigned into A2A KO group and WT group. Another batch of male C57BL/6, specific

  5. Abundance, distribution and trend of the lesser spotted eagle (Aquila pomarina breeding population in Slovakia

    Directory of Open Access Journals (Sweden)

    Dravecký Miroslav

    2015-06-01

    Full Text Available Odhad pocetnosti hniezdnej populácie orla kriklavého na Slovensku bol publikovaný v rokoch 1955-2002. V tejto štúdii odhadujeme pocetnost hniezdnej populácie orla kriklavého k rokom 1997 a 2014. K roku 1997 bol odhadnutý celkový pocet 762 párov. V roku 2002 bol publikovaný odhad pocetnosti k roku 2000 vo výške 800-900 párov. K roku 2014 odhadujeme pocetnost hniezdnej populácie orla kriklavého na Slovensku na 600-800 párov a klesajúci trend vývoja populácie v rokoch 2000-2013 priemerne -23%. Náš odhad je podložený výsledkami extrapolácie údajov, získaných v rokoch 2011-2013 z ôsmich vybraných chránených vtácích území v rámci riešenia projektu LIFE09 NAT/SK/000396 „Ochrana orla kriklavého na Slovensku“. Pri štatistickej analýze pocetnosti, hustôt a trendov pre celý areál hniezdneho rozšírenia druhu na Slovensku sme vychádzali zo sumarizovaných poctov hniezdnych párov, ich hustôt a trendov vo vybraných územiach a z ich stratifikácie podla výsledkov ANOVA a zhlukovej analýzy. Pre 8 chránených vtácích území vcelku (n = 8 i v clenení podla kvadrátov Databanky Fauny Slovenska (n = 119 sme vypocítali intervalové odhady stredných hodnôt poctu párov (prosté i vážené aritmetické priemery a mediány. Ich sumarizáciou pri postupnom zväcšovaní vzorky (n = 8, 9, 11a 12 a extrapoláciou na celý areál hniezdneho rozšírenia druhu na Slovensku (ca 28 935 km2 sa výsledné odhady stredných hodnôt stabilizovali na úrovni 670 (aritmetický priemer s 95%LCL = 507 a 95%UCL = 834 resp. 765 (medián s 95%LCL = 393 a 95%UCL = 841 hniezdnych párov. Ako najspolahlivejší odhad pocetnosti hniezdnej populácie orla kriklavého na Slovensku k roku 2014 vychádza medián 765 párov v 95 %-nom intervale spolahlivosti od 393 do 841 párov a s medzikvartilovým rozpätím od 418 do 835 párov. Odhadujeme 23 %-ný pokles celoslovenskej populácie v rokoch 2000-2013, co predstavuje mierny

  6. Fenomen “krov” u sociolingivstici (s romanskim primjerima

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    Žarko Muljačić

    1984-12-01

    tipa (Švicarska Konfederacija, sa svojim kantonima, koji su ponekad dvojezični i višejezidni. Funkcije "krova" obavljaju jezici po izgradjenosti koji mogu biti dominantni ili kodominantni. U prvom slučaju nalazimo "jednostruki krov" a u drugom "složeni krov". Autoi izražava nadu da će slijepo ili u najmanju ruku nedobrovoljno prihvaćanje "krova" (do nedavna na snazi u centralističkim zemljama, sa reperkusijama koje još nisu uklonjene biti zamijenjeno s dobrovoljnim prihvaćanjem "krova", koji u zajedništvu ne smije biti "jednostruk" nego "složen" (jedna viša forma, koju autor naziva "složeni makro-krov", pokriva uostalom tiho i skoro nevidljivo sve evropske jezike, pa čak i one koji nisu članovi indoevropske jezične obitelji, U romanskom svijetu primjer za "složeni krov" je zasigurno Švicarska (nevolje izazvahe ressentimentom stanovništva novoosnovanog kantona Jura su prolazne prirode. Autor se takodjer ukratko osvrće na recentne pojave etnicističkih pokreta koji za "male" jezike po izgradjenosti u nastajanju nisu opasni ako se radi o stanovništvu koje već ima "svoj" jezik po izgradjenosti, nastao na osnovu forme i supstance istog "jezika po udaljenosti" (njem. Abstandsprache, tal. lingua per distanziazione; sit.venia verbo!. Time se misli na jezične planove pijemontskih, venetskih, lombardskih i sicilijanskih te nekih drugih autonomista (na pr. harpitanskih, u autonomnoj regiji Valle d'Aosta: Oni postaju opasni, i čovjek se ne može oteti dojmu da se radi ponekad o manipullianim pokretima po načelu: Divide et imperal, kad se unutar etničkih grupa koje su za dlaku izbjegle lingvicid ili su bile na najboljem putu da mu jednom podlegnu pojave "novi" jezici (kao što je to slučaj s najnovijim razvojem u većinski katalonskoj regiji valencija ili u nekim dijelovima okcitanije.

  7. Pre- and postsurgical diagnostics with dual-source computed tomography in cardiac surgery; Einsatz der Dual-source-Computertomographie in der prae- und postoperativen kardiochirurgischen Diagnostik

    Energy Technology Data Exchange (ETDEWEB)

    Nikolaou, K.; Saam, T.; Rist, C.; Johnson, T.; Reiser, M.F.; Becker, C.R. [Klinikum Grosshadern der Ludwig-Maximilians-Universitaet Muenchen, Institut fuer Klinische Radiologie, Muenchen (Germany); Vogt, F.; Oberhoffer, M.; Reichart, B. [Ludwig-Maximilians-Universitaet Muenchen, Herzchirurgische Klinik und Poliklinik, Muenchen (Germany)

    2007-04-15

    -source CT shows great diagnostic potential in patients before or after cardiac surgery. DSCT provides a high diagnostic accuracy for detection of coronary artery stenosis before bypass surgery. DSCT also proved to be accurate in the assessment of patients who received aortic valve replacement. (orig.) [German] Ziel der Studie war es festzustellen, in wieweit die Dual-source-Computertomographie (DSCT) verschiedene prae- und postoperative diagnostische Fragestellungen in der Kardiochirurgie zu klaeren vermag. Zwanzig Patienten wurden einer DSCT des Herzens unterzogen. Das CT-Geraet mit 2 rotierenden Roentgenroehren (Somatom Definition, Siemens Medical Solutions, Forchheim, Deutschland) erreicht eine zeitliche Aufloesung von 83 ms. Das Patientenkollektiv bestand aus 2 Subkollektiven: Bei 10 Patienten mit bekannter koronarer Herzkrankheit (KHK) wurden vor einer geplanten Bypassoperation die Ergebnisse der DSCT-Koronarangiographie (CTA) und der quantitativen Katheterangiographie (QCA) verglichen, um die diagnostische Genauigkeit der DSCT zum Nachweis signifikanter Koronarstenosen (>50%) in einem Kollektiv mit grosser Vortestwahrscheinlichkeit fuer das Vorliegen einer KHK zu evaluieren. Bei 10 weiteren Patienten wurde nach Aortenklappenersatz (Homografts) im postoperativen Verlauf die Frage untersucht, ob eine Restenose ausgeschlossen und die Aortenklappenoeffnungsflaeche (AKOeF) im Vergleich zur Echokardiographie korrekt gemessen werden kann. Im Rahmen der praeoperativen Stenosenauswertung wurden 144 von 150 Koronararteriensegmenten (96%) in der CTA als ''beurteilbar'' eingestuft. Laut QCA lag bei allen Patienten eine signifikante KHK mit insgesamt 43 signifikant stenosierten Segmenten vor. Sensitivitaet und Spezifitaet der CTA lagen in der geblindeten Analyse bei 95% (41/43) und 93% (103/111), bei einem positiven und negativen Vorhersagewert (PPV, NPV) von 79% (31/39) und 98% (103/105). Bei den 10 Patienten mit Aortenklappendiagnostik waren alle CT

  8. L'emploi alternative de certains signes, à la languen'est pas absolumais con­ditionné

    Directory of Open Access Journals (Sweden)

    Mihailo Stevanović

    1972-12-01

    Full Text Available L'aoriste et le présent des verbes perfectifs, par exemple, ne sont des oppositions syntaxiques que dans la narration et non, comme on le pensait, dans chaque emploi temporal relatif. Dans les exemples du type: Lujo ogrnū haljinicu, pa se spusti na travui leže -l'aoriste pourrait être substitué par le présent, et dans l'exemple suivant: Uveče ga brat njegov Milutin uzmē te ga iznesē I sàhrani kod crkve- le present pourrait être suhstitué par l'aoriste sans que le sensde ces phrases soit nullement changé. Pourtant dans les cas comme: Mi jedanput Šćepana zàcarismo et Razbolje se Vojvoda Dojčine u Solunu gradu bijelome, le présent ne pourrait pas être employé en fonction de l'aoriste temporel relatif parce que dans le premier exemple il ne s'agit pas de narration et dans le second, malgré le caractère narratif du vers, le présent ne pourrait pas signifier sans ambiguité l'accomplissement en une seule fois de l'action, mais il aurait suggeré sa répétition- significationque l'aoristen'a pas. Le deuxième cas de l'emploi frequent des formes verhales en tautqu'oppositions syntaxiques en serbo-croateest représenté parla function que le présent perfectif et le futur II ont d'exprimer des actions quin'ont pas encore été réalisées ou dont la realization n'est pas certaine. Mais l'emploi alternatif n'est pas absolu ici non plus: il est limité par l'impossibilité du present d'exprimer un accomplissement antérieur à une realization hypothétique de l'action, tandis que le future II, à côté de ce qu'il a en commun avec le présent, il a cette signification aussi (Čim stignemo do vase kuće, odmah ćemo produžiti, ako drugi  budu došli. On cite les constructions avec les prépositions prema et k(a+ datif comme un cas qui montre que l'emploi alternatif est limité. A savoir, ces deux constructions représentent les oppositions syntaxiques au cas où le verbe principal exprime le mouvement en direction du nom employ

  9. Romanian Association of Balneology Conference – 2015, 28 – 31 May, Băile Tuşnad

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    MUNTEANU Constantin

    2015-05-01

    Full Text Available 1.\tProf. Onose G, MD, PhD, MSc, Univ. Assist. Haras MA, MD, PhD, Prof. Sinescu CJ, MD, PhD1,2, Univ. Assist. Daia CO, MD, PhD1,2, RDI Assist. Andone I, MD, Postgrad2, Onose VL, MD,3, Assist. Prof. Capisizu A, MD, PhD1,4, Prof. Grigorean VT, MD, PhD1,2, Assoc. Prof. Ciobotaru C, MD, PhD5,6, Sandu AM, MD, Postgrad.2, Assist. Prof. Blendea CD, MD, PhD7, 8 - BASIC WELLNESS FEATURES AND SOME RELATED ACTIONS, PROPENSIVE INCLUDING FOR ACTIVE AND HEALTHY AGEING - 2.\tOlga SURDU, T.V. SURDU, Monica SURDU - STATE OF ART of balneotherapy/thermalisme in Romania 3.\tIliuta Maria Alexandra - THE IMPORTANCE OF NUTRITION IN HEALTH PROGRAMS 4.\tDr. Dogaru Gabriela - THERAPEUTIC EFFECTS OF MINERAL WATERS IN RENAL DISEASES 5.\tVeryho N.S. - THERAPEUTIC EFFECT OF MINERAL WATER WITH DIFFERENT CONCENTRATIONS OF HUMIC ACIDS IN EXPERIMENTAL HEPATITIS INDUCED BY ACETAMINOPHEN 6.\tIoana Marian - SPA SEEN AS AN EXTENSION OF A HEALTH RESORT - A NECESSARY CONDITION FOR INTEGRATING ROMANIA IN THE LIST OF GLOBAL WELLNESS TOURISM DESTINATIONS 7.\tBilha Neli-Claudia, Bilha Stefan - THE BALNEARY TREATMENT COURSE – A BREATH OF LIFE 8.\tBilha Neli-Claudia, Bilha Stefan - WICH SALT MINE DO YOU RECOMMEND FOR SPELEOTHERAPY? Interdisciplinary project proposal. 9.\tGáspár Boróka, Gabriela Dogaru - BALNEOTHERAPY IN THE BOGHIŞ RESORT 10.\tDr. Luminita Aurelia Pasca - REHABILITATION OF ELDERLY PATIENTS WITH CARDIOVASCULAR DISEASE 11.\tDr. Luminita Aurelia Pasca, Dr. Valer Donca - GEROVITAL – PROPHYLAXIS OF AGING AND GERIATRIC TREATMENT 12.\tAndronache Cristina - IMPORTANCE OF REHABILITATION TREATMENT IN HEMOPHILIC ARTHROPATHY – A CASE REPORT 13.\tDr. Motricala Marieta, Molnar Akos - BALNEOTHERAPY IN BAILE TUSNAD 14.\tIoana Pop, Bianca Pop - MEDICAL REHABILITATION IN NEUROPATHIC PAIN 15.\tAmalia Salca¹, Nicoleta Stoica1, Gabriela Dogaru¹,² - BALNEOTHERAPY IN GONARTHROSIS 16.\tAlexandru Bogdan-Cătălin, Toşa Edith-Éva - USING PULSED SHORT WAVE (DIAPULSE COMPLEMENTARY TO

  10. EFFECT OF DIFFERENT COOLING SYSTEMS ON LYING TIME OF DAIRY COWS IN CUBICLES WITH SEPARATED MANURE SOLIDS BEDDING VPLYV ROZDIELNYCH SYSTÉMOV OCHLADZOVANIA NA DOBU LEŢANIA DOJNÍC USTAJNENÝCH V LEŢISKOVÝCH BOXOCH PODSTIELANÝCH SEPAROVANÝM KALOM HNOJOVICE

    Directory of Open Access Journals (Sweden)

    Jana LENDELOVÁ

    2012-12-01

    sprchový systém s ventilátormi. Aktivity a odpočinok zvierat boli hodnotené v 10 minútových intervaloch pomocou kamerového systému po dobu 24 hodín. K štatistickému vyhodnoteniu získaných údajov bola pouţitá neparametrická Kruskal-Wallisová jednofaktorová analýza rozptylu a mnohonásobný porovnávací test k zisťovaniu preukaznosti rozdielov jednotlivých prejavov správania medzi skupinami dojníc. Zvýšené prúdenie vzduchu ventilátormi malo pozitívny účinok a zosilnilo ochladzovací efekt zvierat vyuţívajúcich evaporačné ochladzovanie sprchovým systémom. Bolo zistené preukazné predĺţenie celkovej doby leţania a skrátenie doby státia. Zvieratá v skupine SV so sprchami a ventilátormi leţali v boxoch dlhšie ako v skupine S so sprchami bez ventilátorov (10.76 h*d-1*ks-1 oproti 7.71 h*d-1*ks-1, P<0.001. Celkové leţanie v skupine SV predstavovalo 11.31 h*d-1*ks-1a v skupine S 10.22 h*d-1*ks-1. Menej ochladzované zvieratá v skupine bez ventilátorov preukazne viac zalíhali do chodby (2.52 h*d-1*ks-1 oproti 0.56 h*d-1*ks-1, P<0.001, a to aj v nočnom období.

  11. Micromammiferi del Parco Regionale di Monte Cucco (Perugia

    Directory of Open Access Journals (Sweden)

    A.M. Paci

    2003-10-01

    >Microtus ?Terricola? sp. riferibili alle specie subterraneus e multiplex che, al momento, si è preferito omettere dall?elenco in quanto oggetto di studi più accurati tutt?ora in corso. Mancano invece, a dispetto dell?atteso, Sorex araneus, i Neomys sp, Microtus arvalis e Rattus rattus, che rappresenteranno sicuramente il tema principale dei prossimi lavori unitamente a Chyonomys nivalis, ancora da accertare in Umbria. È da sottolineare che, nonostante l?indagine non sia stata caratterizzata da metodiche pianificate, si sono ottenute molte informazioni grazie alla disponibilità di alcuni boli di Barbagianni Tyto alba che hanno fornito da soli il 50% delle specie in elenco e permesso di determinare quegli animali, generalmente diffusi ma difficilmente individuabili attraverso indici di campo, quali i topiragno, le arvicole e i topi selvatici.

  12. 硅烷乳液、TEOS和硅溶胶对水泥砂浆防水性能的影响%Effect of silane emulsions,TEOS and silica sol on the water absorption of cement mortar

    Institute of Scientific and Technical Information of China (English)

    张翠; 李绍纯; 赵铁军; 金祖权

    2016-01-01

    Treat the cement mortar with tetraethyl orthosilicate(TEOS),octyl-triethoxy silane emulsions,isobutyl triethoxy silane emulsions and silica sol.The properties of mortar water absorption with different waterproof material was studied.Analyzed the changes of surface contact angle of water in the slurry.The effect of different waterproof net of micro-morphology on cement slurry.The waterproof mechanism of different waterproof materials were studied.Mortar water absorption test results show that TEOS and emulsion mixing effect is best,contrast with untreated mortar,36 h water quantity dealing with 1/9,latex coating times alone,is dealing with 1/7 the size of a sin-gle coating is worst effect of tetraethyl silicate,silica sol,is only 1/2,the processing of but still have certain waterproof function.The con-tact Angle test results show that the net pulp after processing the water contact Angle of not less than 90°.Have good hydrophobicity,TEOS and emulsion after mixing the water contact angle of up to 104°.XRD,SEM results show that the different waterproof material on the net after the net pulp slurry surface,net paste microstructure improved.%选用正硅酸四乙酯(TEOS)、辛基三乙氧基硅烷乳液、异丁基三乙氧基硅烷乳液以及硅溶胶对水泥砂浆进行处理,研究了不同防水材料对砂浆吸水性能的影响,并分析了水在水泥浆将表面接触角的变化,研究了不同防水材料对水泥净浆试块微观形貌的影响,研究了不同防水材料的防水机理。砂浆吸水试验结果表明TEOS和乳液混合使用效果最好,36 h吸水量为未处理砂浆的1/9,单涂乳液次之,是未处理时的1/7,单涂正硅酸四乙酯、硅溶胶效果最差,仅为未处理时的1/2,但仍具有一定防水作用。接触角试验结果表明:净浆经处理后水的接触角均大于90°,具有良好疏水性,TEOS与乳液混合使用后水的接触角可达104°。XRD、SEM结果表明:不同防

  13. Nachweis von Zytomegalie-Virus Immunglobulin G und M Antikrpernaus filtergetrocknetem Neugeborenenblut

    Institute of Scientific and Technical Information of China (English)

    QIAN; Xiaohu

    2001-01-01

    [1]Fowler K B, Stagno S, Pass R F et al. The outcome of congenital cytomegalovirus infection in relation to maternal antibody status. N Engl J Med, 1992,326:663[2]Evengard B, Linder E. Standardization of a filter-paper technique for blood sampling. Ann Trop Med Parasitol,1998,82:295[3]Wassilak S G F, Bernier R H, Herrmann K L et al.Measles seroconfirmation using dried blood specimens on filter paper. Pediatr Infect Dis, 1984,3:117[4]Lebech M, Petersen E. Detection by enzyme immunosorbent assay of Toxoplasma gondii IgG antibodies in dried blood spots on PKU-filter paper from newborn. Scand J Infect Dis, 1995,27: 259[5]Lamy M E, Mulongo K N, Gadisseux J F et al. Prenatal diagnosis of fetal cytomegalovirus infection. Am J Obstet Gynecol, 1992,166 : 91[6]Takkouche B, Iglesias J, Alonso-Fernandez J R et al. Detection of Brucella antibodies in eluted dried blood: a validation study. Immunol Lett, 1995,45:107[7]Matsubara K, Tanabe K, Akane A et al. A novel assay for typing Rh antigens in blood-stains using a lectin specific to the bisecting N- acetyl-D-glucosamine side chain of glycoprotein. J Immunol Methods, 1994,173:175[8]Pappaioanou M, Kashamuka M, Behets F et al. Accurate detection of maternal antibodies to HIV in newborn whole blood dried on filter paper. AIDS, 1993,7:483[9]Major C J, Read S E, Francis R A et al. Comparison of saliva and blood for human immunodeficiency virus prevalence testing. J Infect Dis, 1991,163:699[10]Gil A, Gonzalez A, Dominquez V et al. Detection of antibodies against hepatitis A in blood spots dried on filter paper. Is this a reliable method for epidemiological studies ? Epidemiol-Infect, 1997,118:189[11]Torrela A, Solis R L, Rodriguez N et al. Ultramicro ELISA to the detection of IgM antibodies in Mycobacterium leprae using dry blood samples. Rev Inst Med Trop Sao Paulo, 1994,36:131[12]Stevens R, Pass K, Fuller S et al. Blood spot screening and confirmation tests for Syphilis antibody. J

  14. microRNA regulatory mechanism by which PLLA aligned nanofibers influence PC12 cell differentiation

    Science.gov (United States)

    Yu, Yadong; Lü, Xiaoying; Ding, Fei

    2015-08-01

    Objective. Aligned nanofibers (AFs) are regarded as promising biomaterials in nerve tissue engineering. However, a full understanding of the biocompatibility of AFs at the molecular level is still challenging. Therefore, the present study focused on identifying the microRNA (miRNA)-mediated regulatory mechanism by which poly-L-lactic acid (PLLA) AFs influence PC12 cell differentiation. Approach. Firstly, the effects of PLLA random nanofibers (RFs)/AFs and PLLA films (control) on the biological responses of PC12 cells that are associated with neuronal differentiation were examined. Then, SOLiD sequencing and cDNA microarray were employed to profile the expressions of miRNAs and mRNAs. The target genes of the misregulated miRNAs were predicted and compared with the mRNA profile data. Functions of the matched target genes (the intersection between the predicted target genes and the experimentally-determined, misregulated genes) were analyzed. Main results. The results revealed that neurites spread in various directions in control and RF groups. In the AF group, most neurites extended in parallel with each other. The glucose consumption and lactic acid production in the RF and AF groups were higher than those in the control group. Compared with the control group, 42 and 94 miRNAs were significantly dysregulated in the RF and AF groups, respectively. By comparing the predicted target genes with the mRNA profile data, five and 87 matched target genes were found in the RF and AF groups, respectively. Three of the matched target genes in the AF group were found to be associated with neuronal differentiation, whereas none had this association in the RF group. The PLLA AFs induced the dysregulation of miRNAs that regulate many biological functions, including axonal guidance, lipid metabolism and long-term potentiation. In particular, two miRNA-matched target gene-biological function modules associated with neuronal differentiation were identified as follows: (1) miR-23b, mi

  15. Soil Wind Erosion Risk Assessment in Yulin City Using T-S Fuzzy Neural Network Model%基于T-S模糊神经网络模型的榆林市土壤风蚀危险度评价

    Institute of Scientific and Technical Information of China (English)

    刘新颜; 曹晓仪; 董治宝

    2013-01-01

    Soli wind erosion,one of the major causes of environmental problems such as farmland degradation and desertification,producing large scale of sandstorm,polluting the environment and harming human beings' health in arid and semi-arid regions,is the detachment,entrainment,transport deposition of soil particles caused by wind.It is also a serious socio-economic issue which limits the development in those ecologically fragile areas.In the previous studies,methods for accessing wind erosion are based on land use information.In this article,however,another approach that uses GIS and T-S fuzzy neural network model to access soil wind erosion risk is presented.The approach is applied to a case study of the Yulin City located on the wind-sand transition regions of Mu Us sandy land,Inner Mongolian plateau and loess plateau,northwest China,where most serious expansion of desertification is proceeding.Environmental elements of soil wind erosion were selected and their spatial distribution data were extracted using GIS technology and T-S fuzzy neural network model was constructed after training the sample data of different wind erosion risk level.The model was applied to mapping wind erosion risk after inputting the extracted date.The entire jobs were based on the software of ArcGIS9.3 and MATLAB.The results show that:Firstly,the comparison of the risk assessment with land use information in Yulin City demonstrates that the result is in conformity with actual conditions.T-S fuzzy neural network model can reveal the relationship between risk of soil wind erosion and its environment effectively,and provide the basis for predicting wind erosion.Secondly,environmental elements such as wind energy,vegetation coverage,temperature,precipitation,topography control the distribution properties of wind erosion risk in Yulin City.Thirdly,there are 4 divisions of wind erosion risk in Yulin City:the severe risk level,the intense risk level,the moderate risk level and the slight risk level

  16. Next Generation Sequencing and Its Application in Forensic Genetics%二代测序技术及其在法医遗传学中的应用

    Institute of Scientific and Technical Information of China (English)

    王乐; 叶健; 白雪; 杨帆; 赵兴春

    2015-01-01

    analyzing the STR, SNP, Indel and RNA markers simultaneously, which could be impossible on routinely used PCR-CE platforms because of the limited amount of exhibits. In this article, the authors attempt to describe the basic concepts, developmental history and working principles of NGS to Chinese experts in the general field of forensic science and technologies, and share the updates of NGS-based STR typing, SNP typing and whole mtGenome sequencing during the past two years. Representative NGS platforms including the 454/Roche GS FLX system, the Solexa system, the SOLiDTM system, the Ion PGMTM system and the MiSeq FGxTM system were introduced. Annual statistics of research articles on NGS and forensic NGS were described and trends for related research were analyzed. Finally, perspectives of forensic NGS were presented and possible challenges including data analysis methods, openness of NGS systems and ethical issues were discussed in the hope of providing a reference for related research and applications.

  17. The promotion of cytoskeleton integration and redox in the haemocyte of shrimp Litopenaeus vannamei after the successive stimulation of recombinant VP28.

    Science.gov (United States)

    Wang, Lingling; Sun, Xin; Zhou, Zhi; Zhang, Tao; Yi, Qilin; Liu, Rui; Wang, Mengqiang; Song, Linsheng

    2014-07-01

    VP28 protein has been reported to work as a "vaccine" to protect the host from white spot syndrome, but the detailed mechanism of vaccination with VP28 protein in shrimp is still far from well understood. In the present study, whole transcriptomes of shrimp haemocytes were sequenced using the SOLiD4 platform after the successive VP28 stimulation. Eight single-end fragment libraries were constructed and sequenced in the four groups including the VP28-VP28, PBS-VP28, PBS-PBS and BLANK group, and there were 243,949,667 single-end reads with length of 50bp obtained totally, with 14,800 genes further identified. After reads mapping and transcript assembling, 1027, 1539, 1158, 1091 and 1300 genes in five differentially expressed gene lists were obtained in the comparison of VP28-VP28 versus PBS-VP28, VP28-VP28 versus PBS-PBS, VP28-VP28 versus BLANK, PBS-VP28 versus PBS-PBS and PBS-VP28 versus BLANK, respectively. There were 555 differentially expressed genes responsive to the single VP28 stimulation after grouping the PBS-VP28_BLANK and PBS-VP28_PBS-PBS gene lists, and 269 ones responsive to the successive VP28 stimulation after grouping the VP28-VP28_BLANK, VP28-VP28_PBS-PBS and VP28-VP28_PBS-VP28 gene lists. In the GO enrichment analysis of the genes responsive to the single VP28 stimulation, five immune-related GO terms were observed among 14 increased terms, which included defense response to bacterium, response to stimulus, disruption of cells of other organism, killing of cells of other organism and response to bacterium. It was worth noting that the GO terms, response to stimulus and response to stress, were the most common annotation ones which accounted 28.7% and 18.8% of the total differently expressed genes, respectively. For the genes responsive to the successive VP28 stimulation, terms including actin filament-based movement and myosin heavy chain binding were mostly enriched in the Biological Process and Molecular Function category, respectively. In the

  18. LE STAZIONI DI APPRENDIMENTO: ESEMPI DIDATTICI PER UN APPROCCIO DI TIPO GLOBALE ALL’INSEGNAMENTO DELLA LINGUA ITALIANA A STRANIERI

    Directory of Open Access Journals (Sweden)

    Antonietta Gobbis

    2015-02-01

    Full Text Available Il metodo, che si rifà alle teorie pedagogiche di Maria Montessori (1870-1952, è un esempio di lezione “aperta” fondato su pratiche che attivano la sfera emozionale, sensoriale e relazionale oltre a quella logico-razionale della mente. Nel processo di apprendimento di conoscenze acquisite non solo attraverso l’ascolto delle parole dell’insegnante, ma anche attraverso esperienze scelte autonomamente e consapevolmente da soli e in gruppo, l’ambiente delle stazioni didattiche offre tutto il materiale di sviluppo cognitivo in maniera che sia liberamente fruibile dal discente per favorirne la socializzazione e l’autonomia (secondo il motto di Maria Montessori ‘Aiutami a fare da solo’. A lezione esse non solo offrono varietà, comunicazione e divertimento ma, e in ciò è il loro massimo pregio, differenziazione interna al gruppo degli studenti, che possono, senza alcuna pressione da parte dei compagni, lavorare al proprio ritmo e livello scegliendogli esercizi secondo le preferenze individuali lungo un percorso aperto a canali diversi di apprendimento e orientato ad un vivace interscambio con gli altri.Il nostro contributo fa riferimento a tre esempi di stazioni didattiche legate alle feste che più richiamano l’attenzione degli studenti stranieri sul nostro paese: il Natale e il Carnevale. Tali tematiche mostrano come il modello della stazioni didattiche riesca a rapportarsi al vissuto degli studenti motivandoli al raffronto interculturale. Le abbiamo sperimentate in gruppi di studenti dell’Università delle Lingue Mondiali di Tashkent che frequentano il primo e secondo anno del corso di Italiano, studiato come seconda lingua.   Learning stations: didactic examples for a global teaching method This method, based on Maria Montessori’s educational theories, is an example of “open” lessons based on activities that activate emotions, senses and relationships, besides logic and reason. In the learning process knowledge is

  19. 腮腺Warthin瘤与腮腺多形性腺瘤的超声对照分析%Warthin Tumor of Parotid Pleomorphic Adenomas with Ultrasound Contrast Analysis

    Institute of Scientific and Technical Information of China (English)

    尹存军

    2013-01-01

    目的:对腮腺Warthin瘤与腮腺多形性腺瘤的超声进行对照与分析。方法总结与分析腮腺多形性腺瘤24位患者与腮腺Warthin瘤20位患者在超声方面的特征。结果腮腺多形性腺瘤的24位患者都是单侧并且单发,处于腮腺深叶的患者有9位,处于腮腺浅叶的患者有15位;有6位患者为实性并且是均匀的低回声,呈现为分叶状的患者有9位,有6位患者为混合性回声,有12位患者为实性并且是不均匀的低回声;有4位患者的包膜不完整,有20位患者的包膜比较完整。在腮腺Warthin瘤的20位患者中有24个肿瘤,一侧多发的患者有1位,双侧单发的患者有2位,在包膜方面都比较完整;有14位患者为混合性回声,有6位患者为实质性回声;回声弱低的患者有15位,处于腮腺后下极的患者有18位。结论腮腺多形性腺瘤和腮腺Warthin瘤都有着各自不同的超声特征,按照其规律结合临床中的特点,基本上能满足对腮腺多形性腺瘤与腮腺Warthin瘤的诊断需要,关于最后的定性还需要参照患者的病理学检查。%Objective Warthin tumor of parotid pleomorphic adenomas with ultrasound were compared with analysis .Methods Summary and Analysis of pleomor-phic adenoma 24 patients with parotid Warthin tumor 20 patients in the ultrasound area characteristics .Results Pleomorphic adenoma 24 patients were unilateral and soli-tary,deep lobe of the parotid gland in patients 9,in the superficial lobe of the parotid gland of patients with 15;There are six patients were solid and uniform hypoechoic, presented as lobulated nine patients,six patients with mixed echo,there are 12 patients were solid and is inhomogeneous hypoechoic;there are four patients with incom-plete capsule,20 patients more complete envelope.Warthin tumor in the parotid gland of 20 patients with 24 tumors of patients with multiple side of a bilateral single pa-tient has two,in capsule are

  20. Global Monitoring of Martian Surface Albedo Changes from Orbital Observations

    Science.gov (United States)

    Geissler, P.; Enga, M.; Mukherjee, P.

    2013-12-01

    Martian surface changes were first observed from orbit during the Mariner 9 and Viking Orbiter missions. They were found to be caused by eolian processes, produced by deposition of dust during regional and global dust storms and subsequent darkening of the surface through erosion and transportation of dust and sand. The albedo changes accumulated in the 20 years between Viking and Mars Global Surveyor were sufficient to alter the global circulation of winds and the climate of Mars according to model calculations (Fenton et al., Nature 2007), but little was known about the timing or frequency of the changes. Since 1999, we have had the benefit of continuous monitoring by a series of orbiting spacecraft that continues today with Mars Reconnaissance Orbiter, Mars Odyssey, and Mars Express. Daily synoptic observations enable us to determine whether the surface albedo changes are gradual or episodic in nature and to record the seasons that the changes take place. High resolution images of surface morphology and atmospheric phenomena help identify the physical mechanisms responsible for the changes. From these data, we hope to learn the combinations of atmospheric conditions and sediment properties that produce surface changes on Mars and possibly predict when they will take place in the future. Martian surface changes are particularly conspicuous in low albedo terrain, where even a thin layer of bright dust brightens the surface drastically. Equatorial dark areas are repeatedly coated and recoated by dust, which is later shed from the surface by a variety of mechanisms. An example is Syrtis Major, suddenly buried in bright dust by the global dust storm of 2001. Persistent easterly winds blew much of the dust cover away over the course of the next Martian year, but episodic changes continue today, particularly during southern summer when regional dust storms are rife. Another such region is Solis Planum, south of the Valles Marineris, where changes take place

  1. Water in the soil of the Gale crater on Mars: joint analysis of DAN/MSL and HEND/Mars Odyssey data

    Science.gov (United States)

    Mitrofanov, I. G.; Boynton, W. V.; Harshman, K.; Kuzmin, R.; Lisov, D.; litvak, M. L.; Sanin, A.; Starr, R. D.

    2013-12-01

    The ground water measurements by the instrument DAN onboard NASA's MSL rover (see Mitrofanov et al., 2013 a and b, Litvak et al., 2013 and Sanin et al., 2013) may be considered as the ground truth for the orbital measurements of subsurface water by the HEND instrument onboard NASA's Mars Odyssey orbiter (e.g. see and Boynton et al., 2002 and Mitrofanov et al., 2002). The physical methods of these two investigations are different. DAN is an active neutron instrument, which produces strong pulses of 14.1 MeV neutrons and measures the post-pulse emission of epithermal and thermal neutrons (see Mitrofanov et al., 2012). HEND is mapping the prompt neutron emission from the martian subsurface due to bombardment by galactic cosmic rays. However, both investigations have the same goal: to measure the content of ground water in the shallow subsurface. The Mars Odyssey Mission has been mapping neutrons since February of 2002. Based on orbital data, the driest region on Mars was found to be Solis Planium with an average content of water about 2 wt% within a depth of about 1 meter (e.g. see Mitrofanov et al. 2002, 2004). The area around Gale crater was found to have a much higher content of water in the soil. While the estimated values are model dependent (see Mitrofanov et al 2004 for details), the most likely content of ground water from orbital data around Gale is about 4 - 5 wt%. The first data analysis from DAN, on the other hand, has shown that the best estimation of the average content of ground water in Gale along the traverse of the rover is about 2 wt% (Mitrofanov et al., 2013; Litvak et al., 2013). The rover data suggests a content of ground water a factor of 2 less than that based on the orbital data. Two physical reasons for this discrepancy are considered. The first is based on very different averaging distances for measurements by DAN and by HEND. The DAN value is based on averaging along a 1-km traverse, whereas the HEND value represents an area of about 400 km

  2. Transcriptome sequencing for SNP discovery across Cucumis melo

    Directory of Open Access Journals (Sweden)

    Blanca José

    2012-06-01

    Full Text Available Abstract Background Melon (Cucumis melo L. is a highly diverse species that is cultivated worldwide. Recent advances in massively parallel sequencing have begun to allow the study of nucleotide diversity in this species. The Sanger method combined with medium-throughput 454 technology were used in a previous study to analyze the genetic diversity of germplasm representing 3 botanical varieties, yielding a collection of about 40,000 SNPs distributed in 14,000 unigenes. However, the usefulness of this resource is limited as the sequenced genotypes do not represent the whole diversity of the species, which is divided into two subspecies with many botanical varieties variable in plant, flowering, and fruit traits, as well as in stress response. As a first step to extensively document levels and patterns of nucleotide variability across the species, we used the high-throughput SOLiD™ system to resequence the transcriptomes of a set of 67 genotypes that had previously been selected from a core collection representing the extant variation of the entire species. Results The deep transcriptome resequencing of all of the genotypes, grouped into 8 pools (wild African agrestis, Asian agrestis and acidulus, exotic Far Eastern conomon, Indian momordica and Asian dudaim and flexuosus, commercial cantalupensis, subsp. melo Asian and European landraces, Spanish inodorus landraces, and Piel de Sapo breeding lines yielded about 300 M reads. Short reads were mapped to the recently generated draft genome assembly of the DHL line Piel de Sapo (inodorus x Songwhan Charmi (conomon and to a new version of melon transcriptome. Regions with at least 6X coverage were used in SNV calling, generating a melon collection with 303,883 variants. These SNVs were dispersed across the entire C. melo genome, and distributed in 15,064 annotated genes. The number and variability of in silico SNVs differed considerably between pools. Our finding of higher genomic diversity in wild

  3. 江西庐山自然保护区不同森林植被下土壤的持水性能分析%Analysis of Soil Water-holding Capacity under Different Forest Vegetation in Jiangxi Lushan Nature Reserve

    Institute of Scientific and Technical Information of China (English)

    何艾霏; 于法展; 于晨阳; 沈园园

    2011-01-01

    对庐山自然保护区内6种主要森林植被下枯枝落叶累积量及其吸水量和土壤物理性质、土壤水分的入渗性能进行测定分析.结果表明,该自然保护区森林枯枝落叶层平均累积量为18.67 t/hm2,其平均最大净吸水量为35.75 t/hm2,相当于3.58mm的降雨深度,说明其森林枯枝落叶层涵养水源功能较为显著.6种森林植被下土壤容重的大小顺序为黄山松>玉山毛竹>茅栗、白辛树>青岗栎、化香>山毛榉>马尾松;土壤非毛管孔隙度的大小顺序为马尾松>茅栗、白辛树>山毛榉>玉山毛竹>黄山松>青岗栎、化香;马尾松林下土壤硬度最小,而土壤毛管孔隙度最大;从土壤水分的入渗性能来看,马尾松林涵养水源和理水调洪的功能要远大于其他林地类型,玉山毛竹林地最差.%The litter accumulation and its water absorbing capacity (WAC) of six forest vegetations as well as the physical property and water permeability of soil in Lushan nature reserve were determined. As indicated by the results, the average litter accumulation was 18.67 t/hm2, and its average maximum net WAC was 18.67 t/hm2, equivalent to the precipitation depth of one rainfall process 3.58 mm, indicating that the water-holding function of the forest litter layer was significant. The order of soil bulk density under 6 kinds of forest vegetation could be seen easily: Pinus taiwanensis > Yushan Phyllostachys heterocycla ( Carr. ) > Castanea seguinii Dode and Pterostyrax psilophyllus > Cyclobalanopsis glauca, Platycarya strobilacea Sieb. Et Zucc. > Fagus > Pinus massoniana; soli non-capillary porosity decreased as Pinus massoniana > Castanea seguinii Dode and Pterostyrax psilophyllus > Fagus > Yushan Phyllostachys heterocycla(Carr. ) > Pinus massoniana > Cyclobalanopsis glauca and Platycarya strobilacea Sieb. Et Zucc. ; Pinus massoniana forest' s soil hardness was the smallest while its soil minimum capillary porosity was the

  4. Editorial

    Directory of Open Access Journals (Sweden)

    Javier Hernández-Fernández

    2015-12-01

    rapidez y bajado los costos exponencialmente. Tres tecnologías de NGS son utilizadas masivamente, aunque ya hay disponibles otros métodos que no son igualmente utilizados. 454 GSFlex de Roche, Genome Analyzer o HiSeq de Illumina y SOLiD de Life Technologies que con sus mejoras y su bajo costo han permitido en casi todo el planeta dar inicio a un número inusitado de proyectos que en últimas buscan inventariar todos los genes del mundo. Recientemente el mercado ofrece versiones de equipos NGS con características un poco limitadas en rendimiento y capacidad de secuenciación, pero más fácilmente utilizables por la comunidad de investigadores, y, además, orientados a un segmento de mercado diferente. Entre ellos tenemos: los equipos 454 GS Junior de Roche, MiSeq Personal Sequencer de Illumina e Ion Torrent de Life Technologies

  5. Application values of dual-source CT spectral imaging in the diagnosis of solitary pulmonary nodules%双源CT 能谱成像技术在肺内孤立性结节良恶性鉴别诊断的应用价值

    Institute of Scientific and Technical Information of China (English)

    曾炳亮; 李滋聪; 廖小清; 廖凤翔

    2016-01-01

    Objective To explore the diagnosis and differential diagnosis values of spectral imaging of dual-source CT in soli-tary pulmonary nodules. Methods Total of 82 patients with solitary pulmonary nodules (41 cases of benign pulmonary nodules and 41 cases of malignant pulmonary nodules),confirmed by pathology,underwent dual-source CT routine enhanced and dual energy enhancement scanning,and the corresponding CT values and iodine content were measured. The t test was performed to assess the statistical differences,which was definited with P<0.05. Results The CT value of routine enhanced was lower than that of iodine value chart both in arterial phase and venous phase,but the difference was statistically significant only in venous phase (P<0.001). The malignant group had lower normalized iodine concentration (NIC) than benign group ,the difference was statistically significant (P<0.05). Further ROC curve showed that the NIC had a high degree of sensitivity and specificity in the differential diagnosis be-tween benign and malignant pulmonary nodules. Both groups showed a continuous decline trend from 40keV to 140keV. The dif-ference in CT value was statistically significant both in arterial phase and venous phase between groups(P<0.05). Conclusion The spectral imaging of dual-source CT has great clinical value in the differential diagnosis between benign and malignant pulmonary nodules.%目的:探讨双源CT能谱成像技术对肺内良、恶性肿块鉴别诊断能力。方法回顾性分析经病理证实的82例肺内孤立性肿块(恶性肿瘤及良性肿块各41例)的常规CT增强及双源CT增强动、静脉期资料,记录各期CT值及碘含量。组间行t检验,以P<0.05为结果具有显著差异。结果无论是动脉期还是静脉期,常规增强的CT值均低于碘基图,差异仅在静脉期具有统计学意义(P<0.001)。恶性组标准化平均碘浓度含量低于良性组,组间差异有统计学意义(P<0.05),ROC曲线其

  6. Spomenik ninskom biskupu Francescu Grassiju u Chioggi: prilog najranijoj aktivnosti venecijanskog kipara Paola Callala

    Directory of Open Access Journals (Sweden)

    Damir Tulić

    2014-12-01

    Tagliapietre (Venecija, 1680. – 1747.. U Dalmaciji se relativno skroman opus dosada uočenih Callalovih sastoji od dva mramorna anđela postavljena uz glavni oltar u župnoj crkvi u Vodicama te četiri putta svirača smještena sa strane glavnog oltara, kao i onih na bočnom oltaru u župnoj crkvi u Sutivanu na otoku Braču. No, Callalovu ruku moguće je prepoznati i u jednoj skulpturi iz pozamašnog kiparskog programa na oltaru Presvetog Sakramenta u zadarskoj katedrali. Arhitekturu je 1719. izradio Antonio Viviani dok je bogatu kiparsku dekoraciju najvećim dijelom isklesao Francesco Cabianca (Venecija, 1666. - 1737.. U središnjoj niši oltara smješten je manji mramorni kip Žalosne Bogorodice s mrtvim Kristom u krilu. Mramorna zadarska Pietà teško se uklapa u katalog Francesca Cabiance, pogotovo ako se ima u vidu njegov kip istoimene tematike postavljen oko 1714. nad vrata u klaustru venecijanske crkve I Frari. Cabiancina venecijanska Pietà posjeduje oštrinu poteza dlijeta, određenu grubost izvedbe i robustnost tijela te drugačiju fizionomiju i draperiju od zadarskog djela. No, zadarska se Pietà može uključiti u katalog kipara Paola Callala. Pažljivo modelirani lik žalosne Majke te meko oblikovanu draperiju koja se oko stopala radijalno širi prema van možemo usporediti s kipovima Vjere i Nade na oltaru Sakramenta katedrale u Udine, nastalom nakon 1720. godine. Paralele u modeliranju Kristova tijela, a posebno lica suzdržane mimike, nalazimo na kipu Uskrslog na tabernakulu već spominjanog udineškog oltara, kao i u istovjetnom kipu na tabernakulu oltara župne crkve u Clauzettu, što ga također pripisujem Callalu, ali i na ranijim monumentalnijim primjerima, poput Krista s oltara Preobraženja nastalom 1708. za župnu crkvu u Labinu. Callalov spomenik ninskom biskupu Francescu Grassiju u Chioggi važan je pokazatelj razvoja osobnog stila majstora. Njegov se izričaj tako transformira iz ovog „mladenačkog djela“ punog siline i robusnosti u lirsku

  7. 三氯乙烯在不同土壤中的解吸行为%Study on Desorption Behavior of Trichloroethylene on Different Soils

    Institute of Scientific and Technical Information of China (English)

    邱兆富; 胡林; 何龙; 王治立; 吕树光; 隋倩; 林匡飞

    2013-01-01

    procedures exhibited hysteresis to some extent with respect to preceding sorption ones,and the average HI of all sorbents were greater than 2.0,in which the H2O2-treated soils exhibited the higher hysteresis (HI >4.0).The organic matters and minerals contributed to TCE desorption rates of 74% and 26% for the two types of natural soli in average.However,in the H2O2-treated samples,these rates were 68% and 32%,respectively.These results showed that the TCE desorption contributed by minerals decreased with the increase of the organic carbon content.In addition,the higher the initial TCE concentration was,the lower the TCE desorption contribution by minerals was.In summary,desorption quantity of TCE is not only related to the organic carbon content,but also related to organic carbon structure and micropore-mesopore volumes of minerals.These results can give an insight into the desorption mechanisms and promote an accurate model for the transport,fate and risk assessment of TCE in the subsurface environment.

  8. Three kinds of operation method in the treatment of Chiari Ⅰ malformation with syringomyelia curative effect contrast%三种手术方法治疗Chiari Ⅰ畸形并脊髓空洞的疗效对比

    Institute of Scientific and Technical Information of China (English)

    艾克拜尔·亚里坤; 乌拉别克·毛力提; 刘宇利; 杨小朋

    2014-01-01

    Objective To investigate effects of three operation methods in treatment of Chiari Ⅰ with syringomyelia.Methods Retrospective study of patients betwenJune 2005 and June 2012 in The department of Neurosurgery,People's Hospital of Xinjiang Uygur Autonomous Region for a total of 133 cases patient of Chiari Ⅰ malformation with syrigomyelia.Group A was performed in 20 cases for simple posterior bony decompression; group B was performed in 65 cases for posterior bony decompression + dural enlargement repair;group C was performed in 48 cases for posterior bony decompression + dural enlargement repair + resection of the cerebellar tonsils.To comparison the effect of the operation,syringomyelia resolution and the rate of postoperative complications.Results Outpatient follow up for 6 months-9 years,a total of 118 patients were followed up for.According to the criteria of Tator,A,B,C three groups in the superior,good,poor rate was significant difference (P < 0.05).Promote syringomyelia regression had significant difference(P <0.05).Group B and C have the same effect of operation,better than group A.A,B,C three groups of infection were 1 case,2 cases,1 case; postoperative transient headache were 6 cases,30 cases,25 cases;early postoperative cerebrospinal fliud leakage and subcutaneous effusion were 0 case,8 cases,6 cases ; 1 case of death in group C.The total complication rate had no significant difference (P > 0.05).Conclusion Posterior bony decompression plus dural enlargement repair,posterior bony decompression withdural enlargement repair andResection of the cerebellar tonsils were the same effect,arereliable methods for Chiari Ⅰ with syringomyelia,better than soly posterior bony decompression.%目的 观察三种手术方法治疗ChiariⅠ畸形并脊髓空洞疗效.方法 总结新疆维吾尔自治区人民医院神经外科2005年6月至2012年6月133例ChiariⅠ畸形并脊髓空洞患者,A组行单纯骨性减压20例;B组行

  9. Book Reviews

    Directory of Open Access Journals (Sweden)

    Redactie KITLV

    1997-01-01

    NJ: Africa World Press, 1993. xxv + 115 pp. -Sandra Burr, Gretchen Gerzina, Black London: Life before emancipation. New Brunswick NJ: Rutgers University Press, 1995. xii + 244 pp. -Carlene J. Edie, Trevor Munroe, The cold war and the Jamaican Left 1950-1955: Reopening the files. Kingston: Kingston Publishers, 1992. xii + 242 pp. -Carlene J. Edie, David Panton, Jamaica's Michael Manley: The great transformation (1972-92. Kingston: Kingston Publishers, 1993. xx + 225 pp. -Percy C. Hintzen, Cary Fraser, Ambivalent anti-colonialism: The United States and the genesis of West Indian independence, 1940-1964. Westport CT: Greenwood, 1994. vii + 233 pp. -Anthony J. Payne, Carlene J. Edie, Democracy in the Caribbean: Myths and realities. Westport CT: Praeger, 1994. xvi + 296 pp. -Alma H. Young, Jean Grugel, Politics and development in the Caribbean basin: Central America and the Caribbean in the New World Order. Bloomington: Indiana University Press, 1995. xii + 270 pp. -Alma H. Young, Douglas G. Lockhart ,The development process in small island states. London: Routledge, 1993. xv + 275 pp., David Drakakis-Smith, John Schembri (eds -Virginia Heyer Young, José Solis, Public school reform in Puerto Rico: Sustaining colonial models of development. Westport CT: Greenwood Press, 1994. x + 171 pp. -Carolyn Cooper, Christian Habekost, Verbal Riddim: The politics and aesthetics of African-Caribbean Dub poetry. Amsterdam: Rodopi, 1993. vii + 262 pp. -Clarisse Zimra, Jaqueline Leiner, Aimé Césaire: Le terreau primordial. Tübingen: Gunter Narr Verlag, 1993. 175 pp. -Clarisse Zimra, Abiola Írélé, Aimé Césaire: Cahier d'un retour au pays natal. With introduction, commentary and notes. Abiola Írélé. Ibadan: New Horn Press, 1994. 158 pp. -Alvina Ruprecht, Stella Algoo-Baksh, Austin C. Clarke: A biography. Barbados: The Press - University of the West Indies; Toronto: ECW Press, 1994. 234 pp. -Sue N. Greene, Glyne A. Griffith, Deconstruction, imperialism and the West Indian novel

  10. Analysis of CT differential diagnosis of ovarian fibroma and fibrous theca cell tumor%卵巢纤维瘤和纤维卵泡膜细胞瘤的CT鉴别诊断分析

    Institute of Scientific and Technical Information of China (English)

    黄震升; 王廷洲; 陈诚

    2014-01-01

    目的:分析卵巢纤维瘤和纤维卵泡膜细胞瘤的CT鉴别诊断,为CT诊断水平的提高提供临床依据。方法:选取我院2009年4月至2011年4月收治的33例经病理组织学检查确诊的卵巢纤维瘤及纤维卵泡膜细胞瘤患者,其中卵巢纤维瘤18例,纤维卵泡膜细胞瘤15例,对比两组患者临床特征及CT检查结果。结果:病理组织学检查可以发现,两种肿瘤多集中在患者左下腹部,边缘锐利,包膜清晰,卵巢纤维瘤肿瘤直径较纤维卵泡膜细胞瘤大(P<0.05),且实性较多,纤维卵泡膜细胞瘤实性、囊性各半;两组CT平扫均显示附件区边缘锐利,肿物密度与子宫肌层相似;增强扫描其强化幅度均<20 HU,与子宫肌层对比明显,但两组CT表现无明显差异;CT检查对卵巢纤维瘤、纤维卵泡膜细胞瘤诊断的一致性仅为83.3%、73.3%,误诊率达到21.2%(7/33)。结论:卵巢纤维瘤和纤维卵泡膜细胞瘤的病理组织、CT表现均无明显差异,仅免疫组化分析可进行鉴别诊断,而CT增强检查可将卵巢纤维瘤、纤维卵泡膜细胞瘤与子宫、子宫阔韧带肌瘤有效区分,是指导临床治疗方案选择的可靠方式。%Objectives:To analyze the CT differential diagnosis of ovarian fibroma and fibrous theca cell tumor to provide clinical basis for the improvement of CT diagnosis.Methods:33 cases of ovarian fibroma and fi-brous theca cell tumor patients in our hospital from April 2009 ~April 2011 were selected,including 18 cases of o-varian fibroma and 15 cases of fibrous theca cell tumor.Clinical features and CT findings of the two groups were compared.Results:Through pathological examination it was found that the two kinds of tumor mainly located in left lower abdomen of patients with sharp edge and clear membrane.The diameter of ovarian fibroma was larger and soli-der than fiber theca cell tumor which is composed of half solid and half

  11. Research on the Nature of Thermophilic Anaerobic Ethanol Producer Thermo anaerobacter sp DF3 in Petroleum Reservoirs%一株产乙醇嗜热厌氧油藏微生物ThermoanaerobacterspDF3的性质研究

    Institute of Scientific and Technical Information of China (English)

    兰贵红; 邢钰; 曹毅; 乔代蓉; 邹长军; 邓宇; 张辉; 尹小波

    2012-01-01

    [目的]了解油藏微生物ThermoanaerobacterspDF3的生理生化特性,优化木糖产乙醇培养方案。[方法]利用厌氧分离技术从大港油田油层采出液中分离到一株产乙醇厌氧杆菌DF3采用生理生化鉴定与16SrDNA序列的系统发育学分析其系统发育地位,用气相色谱分析其代谢产物。[结果]菌株DF3是一株严格厌氧的嗜热细菌,呈直杆状,G-菌体大小为O.42μm×(1.60~5.20)μm,单生成对或成串生,产顶端芽孢;生长温度为45~78℃(最适65℃),能利用葡萄糖、木糖、果糖、核糖、甘露糖、阿拉伯糖、蔗糖、半乳糖、乳糖、纤维二糖、松三糖、棉子糖、淀粉等作为底物;其16SrRNA与zpseud。ethanolicw相似性为99.7%发酵葡萄糖与木糖的主要产物为乙醇,培养方案优化后其代谢木糖产乙醇终浓度为2.0g/L。[结论]通过试验证明菌株DF3是目前已知菌株中产乙醇活性较强的菌株之一,在65℃时代谢木糖能产生2.0g/L的乙醇目前代谢木糖高产乙醇的菌株均由国外分离获得,菌株DF3的分离获得为我国研究木质纤维素产乙醇提供了优良的出发菌株。%[Objective] The aim was to study the physiological and biochemical char- acteristics of Thermoanaerobacter sp DF3 in petroleum reservoirs and optimize the culture plan of producing ethanol from xylose. [Method] DF3, an anaerobic bacillus producing ethanol, was isolated from produced liquid from oil layer of Dagang oil field with anaerobic isolation technique. The phylogenetic position was analyzed by physiological and biochemical identification and phylogeny of 16S rDNA sequence. The metabolites were analyzed by gas chromatograph. [Result] The strain DF3 was a strict anaerobic thermophilic bacterium, which was straight in rod shape,and gram negative. Besides, it was 0.42 μmx(1.60-5.20) iJm in length. The strains can be soli- tary,in pairs or string and apical spore usually

  12. Hepatocellular carcinoma. Comparison between gadolinium and ironoxide enhanced MR imaging; Risonanza Magnetica epatica ed epatocarcinoma. Confronto fra risultati ottenuti con mezzo di contrasto paramagnetico (gadolinio) e superparamagnetico (particelle di ossido di ferro)

    Energy Technology Data Exchange (ETDEWEB)

    Castoldi, M.C.; Fauda, V.; Scaramuzza, D.; Vergnaghi, D. [Ospedale S. Carlo Borromeo, Milan (Italy). Servizio di Radiologia

    2000-09-01

    soli 3 casi le sequenze TSE T2 pesate hanno avuto rilevanza diagnostica rispetto a quelle GE. Con tecnica TSE sono risultate pero' ben evidenti alcune caratteristiche morfologiche dei noduli (presenza di capsula, di setti, di componenti a diversa differenziazione cellulare), non riconoscibili nelle immagini GE. L'associazione di sequenze TSE T2 pesate e GE T1-T2* pesate dopo mdc ferroso, si e' rivelata piu' sensibile della RM dinamica con gadolinio condotta con sequenza GE (percentuale di identificazione rispettivamente del 97,7 e 75%). I risultati ottenuti, favorevoli all'impiego di mdc ferrosi nell'epatocarcinoma, sono almeno in parte da mettere in relazione alle nostre condizioni di studio e cioe': 1) alla disponibilita' di campo magnetico da 1,0 T, 2) al tipo di esame di RM eseguito dopo mdc ferrosi (utilizzazione di sequenze GE con pesatura T1-T2* e di immagini assiali e sagittali), 3) alla scarsa rappresentazione percentuale di cirrosi di grado elevato nella casistica.

  13. Indicadores de qualidade de solos de área de mineração e metalurgia de chumbo: II - Mesofauna e plantas Soil quality indicators in lead mining and metalurgy area: II - Mesofauna and plants

    Directory of Open Access Journals (Sweden)

    Yara Jurema Barros

    2010-08-01

    riqueza de grupos de organismos e ocorrência de representantes dos grupos Pseudoscorpiones, Mollusca e Isopoda apenas nesse solo. Os grupos Aracnídeos e Psocoptera também foram considerados bons indicadores ambientais, com incremento de suas populações nos solos com maiores teores de metais pesados (solos 2, 3 e 5, possivelmente devido à menor ocorrência de organismos competidores/predadores desses grupos. Os teores de metais pesados nos indivíduos do grupo Formicidae tiveram relação direta com os teores de Pb extraídos com HNO3 0,5 mol L-1 no solo. Quanto ao acúmulo de metais pesados nas plantas coletadas na área, com exceção do solo 1, todas as espécies encontravam-se sob efeito fitotóxico para Pb e Zn, o que sugere a proibição de pastejo na área.The influence of soil management or the contaminant application to the soil, usually induce to a quicker response in the soil mesofauna than in other pedogenic properties; these organisms are therefore good environmental quality indicators. The aim of this study was to identify and quantify the organisms groups of the soil mesofauna and determine Pb and Zn contents in plants in the mining and metallurgy plant in Adrianópolis (PR,to originate biological indicators of the quality of these solis. At the selected locations the following contamination forms were analized: site 1 - reference (native wood; site 2 - residue incorporated in the profile; sites 3 and 6 - close to the chimneys of the plant, with potential import of particulate matter; site 5 - great waste volume covering the soil. Berlese funnels were utilized to collect samples from the 0 to 5 cm layer (20 funnels x 5 sites x 1 layer x 4 times = 400 samples. After the separation of the mesofauna, the organisms were selected and identified. Ants samples were microwave digested with concentrated HNO3 and the Pb, Cu, Ni and Zn contents were determined by inductively coupled plasma - atomic emission Spectrometry (ICP-AES. Plants from Poaceae family were

  14. 改善氧化应激平衡和逆转NET及5-HTT表达异常涉及文拉法辛的抗抑郁作用%Improving oxidative stress response and reversing abnormal expression of hippocampus NET and 5-HTT involved in anti-depressive effect of venlafaxine

    Institute of Scientific and Technical Information of China (English)

    胡小娅; 费慧芝; 蒋心惠; 刘丹; 邱红梅; 周岐新

    2014-01-01

    目的:探讨文拉法辛抗慢性不可预见刺激所致大鼠抑郁症作用与氧化应激和海马去甲肾上腺素转运体( norepi-nephrine transporter,NET)、5羟色胺转运体(serotonin trans-porter,5-HTT)表达的关系。方法裔SD大鼠80只,根据开场实验得分分为4组,即正常对照组( NG)、模型组( MG)、文拉法辛处理的正常对照组( VNG)和文拉法辛处理的模型组( VMG)。采用孤养结合慢性不可预见刺激( chronic unpre-dictable stress,CUS)方法建立大鼠抑郁模型;给药组每天给予文拉法辛(23.4 mg·kg-1·d-1),NG和MG组每天给予同体积溶剂。采用强迫游泳( force swimming)实验评价大鼠的抑郁行为,生物化学方法检测大鼠血清的超氧化物岐化酶( superoxide dismutase,SOD)活性与丙二醛( malondialdehyde, MDA)含量,实时定量RT-PCR分析海马5-HTT、NET的mR-NA表达, Western blot 测定海马5-HTT、NET 的蛋白表达。结果相对于NG组,MG组大鼠在强迫游泳实验中的不动时间明显增加,SOD活性降低,血清MDA含量升高,海马5-HTT表达明显下降,而NET的表达明显升高。文拉法辛干预能阻遏CUMS诱导的上述变化;文拉法辛处理对正常对照大鼠的行为、SOD、MDA和5-HTT及NET表达无明显影响。结论文拉法辛抑制CUS诱导大鼠出现焦虑抑郁样行为的机制至少部分与其改善脑内氧化应激平衡,逆转5-HTT和NET异常表达有关。%Aim To investigate if anti-depressive effect of venlafaxine was associated with improving oxi-dative stress and expression of hippocampus NET and 5-HTT in rat model induced by CUS. MethodsEighty SD male rats were randomly divided into four groups:model group(MG),normal group(NG), ven-lafaxine-treated normal group ( VNG ) , and venlafax-ine-treated model group ( VMG) . VNG and VMG were given venlafaxine (23. 4 mg·kg-1 ·d-1 ) once daily;NG and MG were given the same volume solvent. Soli-tary condition with chronic unpredicted stress ( CUS ) was taken to

  15. Prikazi

    Directory of Open Access Journals (Sweden)

    Drahomira Gavranović

    2012-02-01

    zbornika jest i tema vlasništva pojedinog primjerka stare knjige. Upravo tu temu obradio je D. Shaw pod naslovom Tko je bio vlasnik ove knjige? Proučavanje porijekla knjiga u europskim razmjerima. Autor naglašava važnost katalogizacije na temelju pojedinog primjerka jer se jedino tako u kataložnom zapisu mogu zabilježiti sve karakteristike i posebnosti pojedinog primjerka građe. Upravo takav postupak opisa potaknuo je razvoj područja kojim se bavi autor u svome radu - proučavanja porijekla knjige. Važnost proučavanja prethodnih vlasnika jasno se vidi u mogućnosti okupljanja raspršenih knjižnica i privatnih zbirki koje svoju „pripadnost“ određenoj zbirci ponekad mogu dokazati jedino obilježjima vlasnika ili knjižnice kojoj su nekoć pripadali. Osim vlastoručnih potpisa vlasnika i pečata knjižnice, dokazi o prijašnjim vlasnicima mogu se istražiti i uz pomoć signatura, ex librisa, pečata na uvezima itd. Veliku pomoć u toj vrsti istraživanja pruža i Konzorcij europskih knjižnica (CERL koji nudi mrežnu bazu podataka o porijeklu knjiga. Posljednji rad u ovoj cjelini izradila je doktorska studentica M. Tomić pod naslovom Podaci o povijesti primjerka u hrvatskim katalozima stare i rijetke građe. Autorica je prikupila dostupne (tiskane kataloge te je kroz te kataloge i kataložne zapise starih i rijetkih knjiga u online katalozima istražila koliko se bilježe podaci o porijeklu primjerka. U radu su prikazani rezultati spomenutog istraživanja s posebnim osvrtom na podatke o povijesti primjerka ukoliko su (i ako su uopće uključeni u tiskane kataloge stare i rijetke građe u hrvatskim knjižnicama te u online katalozima hrvatskih knjižnica. Dodana vrijednost ovoga rada jest i bibliografija kataloga inkunabula, kataloga knjiga i rukopisa koji se nalaze u muzejskim knjižnicama i crkvenim ustanovama te kataloga izložbi starih i rijetkih knjiga. Neki od izvora koji su istraženi nisu ranije uključivani u postojeće bibliografije stare gra