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Sample records for amoreira morus alba

  1. Digestibilidade in vivo dos nutrientes de cultivares de amoreira (Morus alba L. em caprinos

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    Dorigan Cláudia Josefina

    2004-01-01

    Full Text Available Este trabalho foi conduzido para avaliar a digestibilidade "in vivo" dos nutrientes dos cultivares de amoreira FM 86 e FM SM nas idades de crescimento de 45 e 90 dias. Foram utilizados 8 caprinos machos, da raça Saanen, com 6 meses de idade e peso vivo médio de 26kg. O delineamento experimental adotado foi o inteiramente casualizado em esquema fatorial 2 X 2 (2 cultivares de amoreira e 2 idades de crescimento. O coeficiente de digestibilidade da FDN do cultivar FM 86 (74,82 % superou o cultivar FM SM (69,36 %, não ocorrendo diferença significativa entre as idades de crescimento. Ocorreu interação significativa entre cultivar e idade de crescimento para o Coeficiente de Digestibilidade da FDA, e o cultivar FM 86 na idade de 45 dias (75,09 % superou a de 90 dias (68,82 %. Para os parâmetros NDT e oeficientes de digestibilidade da energia, MS e PB, verificou-se superioridade da idade de corte de 45 dias, sem diferença entre os cultivares. Concluiu-se que os cultivares FM 86 e FM SM apresentaram excelente valor energético e altos coeficientes de digestibilidade da MS, da PB e dos constituintes da parede celular, indicando um elevado potencial da amoreira como forrageira para caprinos.

  2. Avaliação nutricional do feno das folhas da amoreira (Morus alba L. em frangos de corte - doi: 10.4025/actascianimsci.v33i4.10679 Nutritional assessment of mulberry (Morus alba L. leaf hay in broilers - doi: 10.4025/actascianimsci.v33i4.10679

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    Euclides Braga Malheiros

    2011-09-01

    Full Text Available O experimento foi conduzido com o objetivo de avaliar nutricionalmente o feno das folhas de amoreira, utilizando-se de frangos de corte. Foram utilizados cinco tratamentos (Testemunha (sem amoreira, 3,16% FB, 15% de amoreira (4,14% FB, 30% de amoreira (5,09% FB, Sem amoreira (4,14% FB e Sem amoreira (5,09% FB usando-se o delineamento em blocos casualizados, com dois blocos e três repetições dentro de cada bloco e avaliados os índices de desempenho, o exame histopatológico dos órgãos viscerais e medidas morfométricas do núcleo dos hepatócitos e ácinos pancreáticos. Foi verificado o pior desempenho produtivo para as aves que ingeriram feno de folhas de amoreira, além de lesões tais como esteatose, proliferação de células de ductos hepáticos e necrose focal múltipla no fígado das aves alimentadas com o tratamento 30% de amoreira (5,09% FB, além da diminuição nas dimensões do núcleo dos hepatócitos e dos ácinos pancreáticos.The trial was carried to evaluate the nutritional effects of mulberry leaf hay in broiler chickens. Five treatments were used: control (no mulberry, 3.16% CF; 15% mulberry (4.14% CF; 30% mulberry (5.09% CF, no mulberry (4.14% CF; no mulberry (5.09% CF. A randomized blocks design was used, with two blocks and three replications into the blocks to evaluate performance index, histopathological examination of the visceral organs and morphometric measurements of the hepatocyte nucleus and pancreatic acini. A poor performance index was observed for broilers feeding on mulberry leaves; lesions such as steatosis, proliferation of hepatic duct cells and multiple necrosis were found in the livers of the chickens fed with 30% mulberry (5.09% CF, as well as size reduction of the hepatocyte nucleus and pancreatic acini. From these data, it is concluded that mulberry probably has some toxic substance which can interfere in the improvement of diet ingredients, resulting in damage to broiler chickens.

  3. Nutrient digestibility of mulberry leaves (Morus alba

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    Riyadh A. Al-Kirshi

    2013-06-01

    Full Text Available The current study was conducted to determine the chemical composition of mulberry (Morus alba leaf meal (MLM and its nutritive value as a feed ingredient. Fifteen layer and fifteen broiler chickens were used in the digestibility trial. The dry matter (DM, crude protein (CP, ash, fat (EE, crude fibre, neutral detergent fibre (NDF, acid detergent fibre (ADF, calcium (Ca, phosphorus (P and gross energy (GE content of MLM were analyzed. The precision-feeding technique was applied to feed the birds 30 g/kg DM of MLM. The results showed that MLM contained a high content of CP (29.8%, Ca (2.73% and NDF (35.77%. Layers and broilers chicken could utilize 73% and 72% of CP, respectively, in MLM. The nutrient digestibility of DM and NDF was higher in layers than in broilers. No significant effect was observed in ME and other nutrient digestibility between the two classes of fowls. In general, the incorporation of MLM into the chickens’ diet could be a good source of protein despite its high fibre content.

  4. Wood Anatomical Structure of Morus alba L. and Morus nigra L., Native to Iran

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    Elham KARAMI

    2010-12-01

    Full Text Available Iran is a wast country with many different tree species. Among those there are two species of Morus genus including alba and nigra. Since long time ago, white mulberrys wood (Morus alba has been used for making musical instruments especially bowl shaped instruments in Iran.. In contrast, black mulberrys wood (Morus nigra has never been used for these types of applications. In order to investigate the possible replacement choices, this study has been carried out to investigate the anatomical differences and similarities between these two species. Wood samples of the two species have been collected from same site and microsections for light microscopic studies and maceration samples have been prepared. The anatomical characteristics were studied according to the IAWA List of Hardwoods. The most important similarities between them are: vessel solitary in short radial multiples or irregular clusters, fiber nonseptate, rays uniseriate and multiseriate type, paratracheal parenchyma, varying from vasicentric to aliform confluent, apotracheal as marginal bands, Rhombic crystals present in rays and sometimes in parenchyma. The main differences are: semi-ring porous distribution of vessels in M. alba, fewer number of vessels and presence of aliform parenchyma in M. nigra. Taking these results into consideration, the most important features of both species are similar and it could be recommended to use the nigra species as well as the alba for making musical instruments.

  5. Wood Anatomical Structure of Morus alba L. and Morus nigra L., Native to Iran

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    Elham KARAMI

    2010-12-01

    Full Text Available Iran is a wast country with many different tree species. Among those there are two species of Morus genus including alba and nigra. Since long time ago, white mulberry�s wood (Morus alba has been used for making musical instruments especially bowl shaped instruments in Iran.. In contrast, black mulberry�s wood (Morus nigra has never been used for these types of applications. In order to investigate the possible replacement choices, this study has been carried out to investigate the anatomical differences and similarities between these two species. Wood samples of the two species have been collected from same site and microsections for light microscopic studies and maceration samples have been prepared. The anatomical characteristics were studied according to the IAWA List of Hardwoods. The most important similarities between them are: vessel solitary in short radial multiples or irregular clusters, fiber nonseptate, rays uniseriate and multiseriate type, paratracheal parenchyma, varying from vasicentric to aliform confluent, apotracheal as marginal bands, Rhombic crystals present in rays and sometimes in parenchyma. The main differences are: semi-ring porous distribution of vessels in M. alba, fewer number of vessels and presence of aliform parenchyma in M. nigra. Taking these results into consideration, the most important features of both species are similar and it could be recommended to use the nigra species as well as the alba for making musical instruments.

  6. Fatty acids composition of Spanish black (Morus nigra L.) and white (Morus alba L.) mulberries.

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    Sánchez-Salcedo, Eva M; Sendra, Esther; Carbonell-Barrachina, Ángel A; Martínez, Juan José; Hernández, Francisca

    2016-01-01

    This research has determined qualitatively and quantitatively the fatty acids composition of white (Morus alba) and black (Morus nigra) fruits grown in Spain, in 2013 and 2014. Four clones of each species were studied. Fourteen fatty acids were identified and quantified in mulberry fruits. The most abundant fatty acids were linoleic (C18:2), palmitic (C16:0), oleic (C18:1), and stearic (C18:0) acids in both species. The main fatty acid in all clones was linoleic (C18:2), that ranged from 69.66% (MN2) to 78.02% (MA1) of the total fatty acid content; consequently Spanish mulberry fruits were found to be rich in linoleic acid, which is an essential fatty acid. The fatty acid composition of mulberries highlights the nutritional and health benefits of their consumption. Copyright © 2015 Elsevier Ltd. All rights reserved.

  7. Phytochemistry, pharmacology, and clinical trials of Morus alba.

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    Chan, Eric Wei-Chiang; Lye, Phui-Yan; Wong, Siu-Kuin

    2016-01-01

    The present review is aimed at providing a comprehensive summary on the botany, utility, phytochemistry, pharmacology, and clinical trials of Morus alba (mulberry or sang shu). The mulberry foliage has remained the primary food for silkworms for centuries. Its leaves have also been used as animal feed for livestock and its fruits have been made into a variety of food products. With flavonoids as major constituents, mulberry leaves possess various biological activities, including antioxidant, antimicrobial, skin-whitening, cytotoxic, anti-diabetic, glucosidase inhibition, anti-hyperlipidemic, anti-atherosclerotic, anti-obesity, cardioprotective, and cognitive enhancement activities. Rich in anthocyanins and alkaloids, mulberry fruits have pharmacological properties, such as antioxidant, anti-diabetic, anti-atherosclerotic, anti-obesity, and hepatoprotective activities. The root bark of mulberry, containing flavonoids, alkaloids and stilbenoids, has antimicrobial, skin-whitening, cytotoxic, anti-inflammatory, and anti-hyperlipidemic properties. Other pharmacological properties of M. alba include anti-platelet, anxiolytic, anti-asthmatic, anthelmintic, antidepressant, cardioprotective, and immunomodulatory activities. Clinical trials on the efficiency of M. alba extracts in reducing blood glucose and cholesterol levels and enhancing cognitive ability have been conducted. The phytochemistry and pharmacology of the different parts of the mulberry tree confer its traditional and current uses as fodder, food, cosmetics, and medicine. Overall, M. alba is a multi-functional plant with promising medicinal properties.

  8. Evaluation of anti-inflammatory activity of prenylated substances isolated from Morus alba and Morus nigra.

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    Zelová, Hana; Hanáková, Zuzana; Čermáková, Zuzana; Šmejkal, Karel; Dalĺ Acqua, Stefano; Babula, Petr; Cvačka, Josef; Hošek, Jan

    2014-06-27

    Chromatographic separation of root extracts of Morus alba and M. nigra led to the identification of the 2-arylbenzofurans moracin C (1), mulberrofuran Y (2), and mulberrofuran H (3), and the prenylated flavonoids kuwanon E (4), kuwanon C (5), sanggenon H (6), cudraflavone B (7), and morusinol (8), and the Diels-Alder adducts soroceal (9), and sanggenon E (10). The cytotoxicity and their antiphlogistic activity, determined as the attenuation of the secretion of TNF-α and IL-1β and the inhibition of NF-κB nuclear translocation in LPS-stimulated macrophages, were evaluated for compounds 1-10.

  9. Quali-quantitative analyses of Flavonoids of Morus nigra L. and Morus alba L. (Moraceae) fruits.

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    Pawlowska, Agata Maria; Oleszek, Wieslaw; Braca, Alessandra

    2008-05-14

    Morus nigra L., belonging to the Moraceae family, is a decidious tree widely cultivated in Europe and West Asia. It has a long history of medicinal use in Chinese medicine, as a remedy for many kinds of diseases. The aim of the present study was to evaluate the profile of the phenolic constituents of M. nigra fruits and to compare their content with the fruits of another species of Morus, Morus alba, which is also very well known in folklore medicine. The fruits of black and white mulberries have been studied, and five compounds from the methanol extract have been identified by means of HPLC/PDA/ESI-MS. Four compounds (quercetin 3- O-glucoside, quercetin 3- O-rutinoside, kaempferol 3- O-rutinoside, and 5- O-caffeoylquinic acid) have been isolated by use of Sephadex LH-20 column chromatography and HPLC and characterized by means of NMR and ESI-MS. Furthermore, HPLC/PDA/ESI-MS analysis of the red pigment of M. nigra fruits revealed the presence of four anthocyanins recognized as cyanidin 3- O-glucoside, cyanidin 3- O-rutinoside, pelargonidin 3- O-glucoside, and pelargonidin 3- O-rutinoside. All of the compounds were quantified.

  10. Detecção de Meloidogyne enterolobii em mudas de amoreira (Morus nigra L.

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    Vanessa dos Santos Paes-Takahashi

    2015-05-01

    Full Text Available O comércio de mudas sem certificação contribui para disseminação de pragas e doenças, que podem causar sérios danos às plantas cultivadas. Na região de Itapetininga (SP, foram apreendidas, pela equipe da Defesa Agropecuária, mudas de aceroleira, goiabeira e amoreira, comercializadas em caminhões, que apresentavam galhas nas raízes, sintoma típico causado por Meloidogyne spp. A identificação da espécie foi feita através da morfologia da configuração perineal de fêmeas e região labial de machos, bem como através da caracterização do fenótipo enzimático de esterase. Foi constatada a presença de M. enterolobii nas amostras analisadas. Trata-se do primeiro relato do parasitismo de M. enterolobii em mudas de amoreira no mundo.

  11. COMPARATIVE ANTIOXIDANT ACTIVITY OF BERRIES OF MORUS MGRA L., MORUS ALBA L. AND MORUS RUBRA L.

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    Yu. A. Vakhrusheva

    2015-01-01

    Full Text Available The article discusses the results of antiox-idaut activity of berries extract of Moms nigra L., Moras alba L., and Moras rubra L. We have studied the total content of antioxidants in the extract of these species beiiies using liquid chromotographer "Tsvet Yauza-01-AA". Aniperometric method was used for measuring of mass concentration of antioxidants. We determined the quantity of antioxidants using analytical curve of dependence of exit signal on the quercetine and gallic acid concentration. The study resulted on the revelation of antioxidant content in alcohol, water-alcohol, and water extracts from the objects of study mentioned above

  12. CARDIOPROTECTIVE EFFECT OF MORUS ALBA L. LEAVES IN ISOPRENALINE INDUCED RATS

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    S. Madhumitha and A. Indhuleka*

    2012-05-01

    Full Text Available The study was designed to evaluate the cardioprotective effect of methanolic extract of Morus alba L. leaves against isoprenaline- induced myocardial infarction and was investigated by an in vivo method in rats. Male Wistar albino rats were divided into four groups (n=6. Group I rats served as normal control. Group II rats served as isoprenaline induced toxic control (110 mg/kg body weight which was injected intraperitoneally (i.p. for two consecutive days (14th and 15th days. Group III rats were given Morus alba intragastric intubation (500 mg/kg body weight for 15 days. Group IV rats were also given Morus alba as in Group III and additionally isoprenaline was given for two consecutive days (14th and 15th days.The results described the cardioprotective effect that was observed in Group IV which showed a significant (P< 0.05 decreased levels of TBARS and enhanced the activities of both enzymatic and non-enzymatic antioxidants (SOD, CAT, GPx and GSH in myocardial infarcted rats when compared to Groups II and III. In serum, the biomarkers (LDH, CK activities were significantly (P< 0.05 increased in Group II compared to pretreated Group IV. Histopathological studies were also co-relating with the above biochemical parameters. These findings concluded the cardioprotective effect of Morus alba on lipid peroxidation and antioxidant defense system during isoprenaline -induced myocardial infarction in rats.

  13. Formulation and evaluation of antimicrobial activity of Morus alba sol-gel against periodontal pathogens

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    Shilpa Gunjal

    2015-01-01

    Full Text Available Background: Periodontitis has a multifactorial etiology, with primary etiologic agents being pathogenic bacteria that reside in the subgingival area. Recent advances in the field of alternative medicine introduced various herbal products for the treatment of periodontitis. Aim: To assess and compare the antimicrobial activity of Morus alba sol-gel with chlorhexidine sol-gel against ATCC standard strains of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia. Materials and Methods: Crude extract of Morus alba leaves was prepared by Soxhlet method by using ethanol as a solvent. Phytochemical screening of the crude extract of M. alba was performed to check the various chemical constituents. M. alba sol-gel and chlorhexidine sol-gel were formulated using Pluronic f127 and Pluronic f108 and compared for their antimicrobial activity. The minimum inhibitory concentration of both the gels was performed using agar well diffusion technique. Results: The minimum inhibitory concentration of M. alba sol-gel and chlorhexidine sol-gel against A. actinomycetemcomitans is 19 and 17 mm, T. forsythia is 12 and 21 mm, and P. gingivalis is 16 and 18 mm, respectively. Conclusion: Both M. alba and chlorhexidine sol-gel exhibited potent antimicrobial activity against periodontal pathogens.

  14. UV-B Induced Changes in the Secondary Metabolites of Morus alba L. Leaves

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    Da-Wei Zhang; Jing-Kui Tian; Run-Ze Chen; Lei Cui; Hong-Wei Fu; Lin Zhang; Ming-Yao Sun; Xi-Da Gu

    2010-01-01

    Ultraviolet-B (UV-B) radiation is harmful to plants and human beings. Many secondary metabolites, like flavonoids, alkaloids, and lignin, are UV-B absorbing compounds, which can protect the genetic material of plants. Furthermore, they are active components of herbal drugs. UV-B radiation can activate the self-protective secondary metabolism system. The results of this paper provide a method to induce bioactive secondary metabolites from mulberry leaves (Morus alba L.) by UV-B irradiation in ...

  15. ANTI-DIABETIC EFFECT OF MORUS ALBA ON RABBIT AS ANIMAL MODEL

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    Laddha G. P.

    2012-04-01

    Full Text Available A study of ancient literature indicates that diabetes was fairly well known and well conceived as an entity in India. The nature has provided abundant plant wealth for all the living creatures, which possess medicinal virtues. Therefore, there is a necessity to explore their uses and to conduct Pharmacognostic and pharmacological studies to ascertain their therapeutic properties. In fact, nowadays diabetes is a global problem. Hence, the present study aims to open new avenues for the improvement of medicinal uses of Morus alba. for the area for diabetes. Another important objective of such study is to bring the anti-diabetic medicinal plants sector on a firm scientific footing, raise awareness and add value to the resource. Dried petroleum ether (60-80°C extracts of leaves of Morus alba. were subjected for hypoglycemic activity in New Zealand rabbits (1.5-3.5 kg. Blood sugar level was determined using digital glucometer. The oral administration of leaf extracts at doses of 200 mg/ kg− lead to a significant blood glucose reduction. This laid the foundation to study the active compounds of such anti-diabetic plants that are responsible for the hypoglycemic activities. It also proves the traditional claim of Kachh region with regard to Morus Alba for its anti-diabetic activity.

  16. Colonization with Arbuscular Mycorrhizal Fungi Promotes the Growth of Morus alba L. Seedlings under Greenhouse Conditions

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    Nan Lu

    2015-03-01

    Full Text Available Morus alba L. is an important tree species planted widely in China because of its economic value. In this report, we investigated the influence of two arbuscular mycorrhizal fungal (AMF species, Glomus mosseae and Glomus intraradices, alone and together, on the growth of M. alba L. seedlings under greenhouse conditions. The growth parameters and physiological performance of M. alba L. seedlings were evaluated 90 days after colonization with the fungi. The growth and physiological performance of M. alba L. seedlings were significantly affected by the AMF species. The mycorrhizal seedlings were taller, had longer roots, more leaves and a greater biomass than the non-mycorrhizae-treated seedlings. In addition, the AMF species-inoculated seedlings had increased root activity and a higher chlorophyll content compared to non-inoculated seedlings. Furthermore, AMF species colonization increased the phosphorus and nitrogen contents of the seedlings. In addition, simultaneous root colonization by the two AMF species did not improve the growth of M. alba L. seedlings compared with inoculation with either species alone. Based on these results, these AMF species may be applicable to mulberry seedling cultivation.

  17. Elaboración de Bebida Filtrante de la Hoja Morus Alba L con Efecto Hipoglucemiante

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    Ccopa Ihui, Giovanna; Universidad Alas Peruanas; Quispe Cruz, Ruth; Fernández Ccapatinta, Karina

    2008-01-01

    En el presente trabajo de investigación se evaluó el efecto reductor sobre la hiperglucemia (niveles de azúcar altos) del Morus alba L. (mora blanca) en ratas inducidas a la diabetes experimental a nivel de laboratorio.Luego de las pruebas bioquímicas con respecto al efecto hipoglucemiante (niveles de azúcar bajos) de la mora, se concluyó que en su composición intervienen flavonoides, sustancias que participan a nivel celular en la disminución de azúcar en la sangre.Con esta información se bu...

  18. Six new record species of whiteflies (Hemiptera: Aleyrodidae) infesting Morus alba in China.

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    Wang, Ji-Rui; Song, Zao-Qin; Du, Yu-Zhou

    2014-01-01

    To determine the species of whiteflies occurring on mulberry, Morus alba L. (Rosales: Moraceae) in China, we collected samples in more than 87 sites in 16 provinces of China from 2008 to 2011. In total, 10 species, representing seven genera of the subfamily Aleyrodinae, were identified. Of these, six species are newly recorded on mulberry in China, namely, Aleuroclava ficicola Takahashi, Aleuroclava gordoniae (Takahashi), Aleurotrachelus camelliae (Kuwana), Bemisia afer (Priesner & Hosny), Bemisia tabaci Gennadius, and Pealius machili Takahashi. Information on the taxonomy, distribution, and host plants of the whitefly species found on mulberry in China, along with a brief description and illustrations of each species are provided.

  19. Chalcone-derived Diels-Alder adducts as NF-κB inhibitors from Morus alba.

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    Phung, Thi Xuan Binh; Tran, Thi Hong Hanh; Dan, Thi Thuy Hang; Chau, Van Minh; Hoang, Thanh Huong; Nguyen, Tien Dat

    2012-01-01

    A bioassay-guided phytochemical fractionation of the methanol extract of the Morus alba root barks led to the isolation of two chalcone-derived Diels-Alder adducts (1 and 2). Their structures were elucidated as kuwanon J 2,4,10″-trimethyl ether (1) and kuwanon R (2) by means of spectroscopic methods. Both compounds strongly inhibited nuclear transcription factor.κB activity with the IC₅₀ values of 4.65 and 7.38 μM, respectively.

  20. Human colon cancer HT-29 cell death responses to doxorubicin and Morus Alba leaves flavonoid extract.

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    Fallah, S; Karimi, A; Panahi, G; Gerayesh Nejad, S; Fadaei, R; Seifi, M

    2016-03-31

    The mechanistic basis for the biological properties of Morus alba flavonoid extract (MFE) and chemotherapy drug of doxorubicin on human colon cancer HT-29 cell line death are unknown. The effect of doxorubicin and flavonoid extract on colon cancer HT-29 cell line death and identification of APC gene expression and PARP concentration of HT-29 cell line were investigated. The results showed that flavonoid extract and doxorubicin induce a dose dependent cell death in HT-29 cell line. MFE and doxorubicin exert a cytotoxic effect on human colon cancer HT-29 cell line by probably promoting or induction of apoptosis.

  1. Three New Isoprenylated Flavonoids from the Root Bark of Morus alba

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    Jae-Woo Jung

    2016-08-01

    Full Text Available Phytochemical investigation of the root bark of Morus alba has led to the isolation and identification of three new isoprenylated flavonoids, namely sanggenon U (1, sanggenon V (2, and sanggenon W (3, along with four known isoprenylated flavonoids: euchrenone a7 (4, sanggenon J (5, kuwanon E (6, and kuwanon S (7. All compounds were isolated by repeated silica gel (SiO2, octadecyl SiO2 (ODS, and Sephadex LH-20 open column chromatography. The structure of the compounds were determined based on spectroscopic analyses, including nuclear magnetic resonance (NMR, mass spectrometry (MS, circular dichroism (CD, and infrared (IR. In addition, compounds 1–4 were isolated for the first time from the root bark of M. alba in this study.

  2. In vitro antioxidant and antimicrobial activity of extracts from Morus alba L. leaves, stems and fruits.

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    Wang, Wei; Zu, Yuangang; Fu, Yujie; Efferth, Thomas

    2012-01-01

    In this study, the aqueous and ethanolic extracts (leaves, stems and fruits) from Morus alba L., a traditional Chinese medicine, were evaluated for their antioxidant and antimicrobial properties. Ethanolic extracts showed higher contents of both total phenolics and flavonoids than aqueous extracts. The total phenolic content was in the order of: leaf extracts > fruit extracts > stem extracts, whereas the total flavonoids was: leaf extracts > stem extracts > fruit extracts. Using DPPH assays, the concentrations providing 50% inhibition (IC(50)) values of aqueous extracts from leaves, stems and fruits were 7.11 ± 1.45 mg/ml, 86.78 ± 3.21 mg/ml and 14.38 ± 2.83 mg/ml, respectively, whereas the IC(50) values of ethanolic extracts were 3.11 ± 0.86 mg/ml, 14.62 ± 2.45 mg/ml and 12.42 ± 2.76 mg/ml, respectively. In sum, the antioxidant activities of ethanolic extracts from M. alba L. were stronger than the aqueous extracts, and in the order of: leaf extracts > fruit extracts > stem extracts. The ethanolic extracts exhibited moderate antimicrobial activities, whereas the aqueous extracts showed poor antimicrobial properties in our test system. This study validated the medicinal potential of M. alba L.

  3. Improved Chemotherapeutic Activity by Morus alba Fruits through Immune Response of Toll-Like Receptor 4

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    Bo Yoon Chang

    2015-10-01

    Full Text Available Morus alba L. fruits have long been used in traditional medicine by many cultures. Their medicinal attributes include cardiovascular, hepatoprotective, neuroprotective and immunomodulatory actions. However, their mechanism of macrophage activation and anti-cancer effects remain unclear. The present study investigated the molecular mechanisms of immune stimulation and improved chemotherapeutic effect of M. alba L. fruit extract (MFE. MFE stimulated the production of cytokines, nitric oxide (NO and tumor necrosis factor-α (TNF-α and tumoricidal properties of macrophages. MFE activated macrophages through the mitogen-activated protein kinase (MAPKinase and nuclear factor-κB (NF-κB signaling pathways downstream from toll-like receptor (TLR 4. MFE was shown to exhibit cytotoxicity of CT26 cells via the activated macrophages, even though MFE did not directly affect CT26 cells. In a xenograft mouse model, MFE significantly enhanced anti-cancer activity combined with 5-fluorouracil and markedly promoted splenocyte proliferation, natural killer (NK cell activity, cytotoxic T lymphocyte (CTL activity and IFN-γ production. Immunoglobulin G (IgG antibody levels were significantly increased. These results indicate the indirect anti-cancer activity of MFE through improved immune response mediated by TLR4 signaling. M. alba L. fruit extract might be a potential anti-tumor immunomodulatory candidate chemotherapy agent.

  4. Improved Chemotherapeutic Activity by Morus alba Fruits through Immune Response of Toll-Like Receptor 4.

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    Chang, Bo Yoon; Kim, Seon Beom; Lee, Mi Kyeong; Park, Hyun; Kim, Sung Yeon

    2015-10-13

    Morus alba L. fruits have long been used in traditional medicine by many cultures. Their medicinal attributes include cardiovascular, hepatoprotective, neuroprotective and immunomodulatory actions. However, their mechanism of macrophage activation and anti-cancer effects remain unclear. The present study investigated the molecular mechanisms of immune stimulation and improved chemotherapeutic effect of M. alba L. fruit extract (MFE). MFE stimulated the production of cytokines, nitric oxide (NO) and tumor necrosis factor-α (TNF-α) and tumoricidal properties of macrophages. MFE activated macrophages through the mitogen-activated protein kinase (MAPKinase) and nuclear factor-κB (NF-κB) signaling pathways downstream from toll-like receptor (TLR) 4. MFE was shown to exhibit cytotoxicity of CT26 cells via the activated macrophages, even though MFE did not directly affect CT26 cells. In a xenograft mouse model, MFE significantly enhanced anti-cancer activity combined with 5-fluorouracil and markedly promoted splenocyte proliferation, natural killer (NK) cell activity, cytotoxic T lymphocyte (CTL) activity and IFN-γ production. Immunoglobulin G (IgG) antibody levels were significantly increased. These results indicate the indirect anti-cancer activity of MFE through improved immune response mediated by TLR4 signaling. M. alba L. fruit extract might be a potential anti-tumor immunomodulatory candidate chemotherapy agent.

  5. Odisolane, a Novel Oxolane Derivative, and Antiangiogenic Constituents from the Fruits of Mulberry (Morus alba L.).

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    Lee, Seoung Rak; Park, Jun Yeon; Yu, Jae Sik; Lee, Sung Ok; Ryu, Ja-Young; Choi, Sang-Zin; Kang, Ki Sung; Yamabe, Noriko; Kim, Ki Hyun

    2016-05-18

    Mulberry, the fruit of Morus alba L., is known as an edible fruit and commonly used in Chinese medicines as a warming agent and as a sedative, tonic, laxative, odontalgic, expectorant, anthelmintic, and emetic. Systemic investigation of the chemical constituents of M. alba fruits led to the identification of a novel oxolane derivative, (R*)-2-((2S*,3R*)-tetrahydro-2-hydroxy-2-methylfuran-3-yl)propanoic acid (1), namely, odisolane, along with five known heterocyclic compounds (2-6). The structure of the new compound was elucidated on the basis of HR-MS, 1D and 2D NMR ((1)H-(1)H COSY, HSQC, HMBC, and NOESY) data analysis. Compound 1 has a novel skeleton that consists of 8 carbon units with an oxolane ring, which until now has never been identified in natural products. The isolated compounds were subjected to several activity tests to verify their biological function. Among them, compounds 1, 3, and 5 significantly inhibited cord formation in HUVECs. The action mechanism of compound 3, which had the strongest antiangiogenic activity, was mediated by decreasing VEGF, p-Akt, and p-ERK protein expression. These results suggest that compounds isolated from M. alba fruits might be beneficial in antiangiogenesis therapy for cancer treatment.

  6. Characterization of a New Flavone and Tyrosinase Inhibition Constituents from the Twigs of Morus alba L.

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    Long Zhang

    2016-09-01

    Full Text Available The twigs of Morus alba L. were found to show strong tyrosinase inhibition activity, and the responsible active components in the extract were further investigated in this study. A flavone, named morusone (1, and sixteen known compounds 2–17 were isolated from M. alba twigs and their structures were identified by interpretation of the corresponding ESI-MS and NMR spectral data. In the tyrosinase inhibitory test, the compounds steppogenin (IC50 0.98 ± 0.01 µM, 2,4,2′,4′-tetrahydroxychalcone (IC50 0.07 ± 0.02 µM, morachalcone A (IC50 0.08 ± 0.02 µM, oxyresveratrol (IC50 0.10 ± 0.01 µM, and moracin M (8.00 ± 0.22 µM exhibited significant tyrosinase inhibition activities, much stronger than that of the positive control kojic acid. These results suggest that M. alba twig extract should served as a good source of natural tyrosinase inhibitors for use in foods as antibrowning agents or in cosmetics as skin-whitening agents.

  7. New methodologies for the extraction and fractionation of bioactive carbohydrates from mulberry (Morus alba) leaves.

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    Rodríguez-Sánchez, Sonia; Ruiz-Aceituno, Laura; Sanz, María L; Soria, Ana C

    2013-05-15

    Pressurized liquid extraction (PLE) was applied for the first time to extract bioactive low molecular weight carbohydrates (iminosugars and inositols) from mulberry ( Morus alba ) leaves. Under optimized conditions, PLE provided a similar yield to the conventional process used to extract these bioactives, but in less time (5 vs 90 min). To remove carbohydrates that interfere with the bioactivity of iminosugars from PLE extracts, two fractionation treatments were evaluated: yeast ( Saccharomyces cerevisiae ) incubation and cation-exchange chromatography (CEC). Both methods allowed complete removal of major soluble carbohydrates (fructose, glucose, galactose, and sucrose), without affecting the content of mulberry bioactives. As an advantage over CEC, the yeast treatment preserves bioactive inositols, and it is an affordable methodology that employs food grade solvents. This work found PLE followed by yeast treatment to be an easily scalable and automatable procedure that can be implemented in the food industry.

  8. Plant propagation of Morus alba var. Criolla using Temporary Immersion Systems

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    J. E. Salas

    2011-04-01

    Full Text Available The mulberry (Morus alba L. it is a forage plant of great economic importance. The present work was carried out with the purpose of to in vitro propagate the variety ‘Criolla’ via organogenesis with the use of Temporary Immersion Systems. It were defined the media and culture conditions for the temporary immersion systems. The results demonstrated that it was possible to obtain a high multiplication coefficient of 15.5. The immersion frequency had a significant influence on the evaluated variables. Maximum values were observed in the number of buds, the longitude of the buds and the multiplication coefficient with four immersions per day. The plants in vitro cultivated in temporary immersion systems were established in the acclimatization phase in a substrate compound for 85% worm humus and 15% zeolita. It were superior in the morphological variables evaluated regarding those propagated by the conventional method of stakes. Key words: multiplication coefficient, in vitro plants, morphologic variations

  9. Antidiabetic and antioxidant effects and phytochemicals of mulberry fruit (Morus alba L.) polyphenol enhanced extract.

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    Wang, Yihai; Xiang, Limin; Wang, Chunhua; Tang, Chao; He, Xiangjiu

    2013-01-01

    The antidiabetic and antioxidant activities of the ethyl acetate-soluble extract (MFE) of mulberry fruit (Morus alba L.) were investigated. In vitro, MFE showed potent α-glucosidase inhibitory activity and radical-scavenging activities against DPPH and superoxide anion radicals. In vivo, MFE could significantly decrease fasting blood glucose (FBG) and glycosylated serum protein (GSP), and increase antioxidant enzymatic activities (SOD, CAT, GSH-Px) in streptozotocin (STZ)-induced diabetic mice. Bioactivity-guided fractionation of the MFE led to the isolation of 25 phenolic compounds, and their structures were identified on the basis of MS and NMR data. All the 25 compounds were isolated from mulberry fruit for the first time. Also, the α-glucosidase inhibitory activity and antioxidant activity of the phenolics were evaluated. Potent α-glucosidase inhibitory and radical-scavenging activities of these phenolics suggested that they may be partially responsible for the antidiabetic and antioxidant activities of mulberry fruit.

  10. Antidiabetic and antioxidant effects and phytochemicals of mulberry fruit (Morus alba L. polyphenol enhanced extract.

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    Yihai Wang

    Full Text Available The antidiabetic and antioxidant activities of the ethyl acetate-soluble extract (MFE of mulberry fruit (Morus alba L. were investigated. In vitro, MFE showed potent α-glucosidase inhibitory activity and radical-scavenging activities against DPPH and superoxide anion radicals. In vivo, MFE could significantly decrease fasting blood glucose (FBG and glycosylated serum protein (GSP, and increase antioxidant enzymatic activities (SOD, CAT, GSH-Px in streptozotocin (STZ-induced diabetic mice. Bioactivity-guided fractionation of the MFE led to the isolation of 25 phenolic compounds, and their structures were identified on the basis of MS and NMR data. All the 25 compounds were isolated from mulberry fruit for the first time. Also, the α-glucosidase inhibitory activity and antioxidant activity of the phenolics were evaluated. Potent α-glucosidase inhibitory and radical-scavenging activities of these phenolics suggested that they may be partially responsible for the antidiabetic and antioxidant activities of mulberry fruit.

  11. Involvement of histaminergic system in the anxiolytic-like activities of Morus alba leaves in mice.

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    Lee, Seungheon; Kim, Dong Hyun; Lee, Ji Hye; Ko, Eun Seong; Oh, Won Bo; Seo, Yong Taek; Jang, Young Pyo; Ryu, Jong Hoon; Jung, Ji Wook

    2013-01-01

    The aim of this study was to identify the effects of 85% methanolic extract of Morus alba leaves (EMA), which is a traditional herb, in mice. The effects of EMA on the anxiolytic-like behaviour were studied using the elevated plus maze (EPM) and hole-board test. To elucidate the mode of action of the anxiolytic-like effects of EMA, the mice were subjected to the co-administration of EMA (200 mg/kg, per os (p.o.)) and either antagonist. EMA (at 200 or 400 mg/kg) significantly increased the percentages of time-spent in the open arms and entries into the open arms of the EPM versus vehicle-treated control group (phistaminergic system in central nerve system.

  12. Neutralization of local and systemic toxicity of Daboia russelii venom by Morus alba plant leaf extract.

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    Chandrashekara, K T; Nagaraju, S; Nandini, S Usha; Kemparaju, K

    2009-08-01

    Antivenom therapy is the current best therapy available for the treatment of fatal snake envenomation. However, the antivenom offers less or no protection against local effects such as extensive edema, hemorrhage, dermo-, myonecrosis and inflammation at the envenomed region. Viperidae snakes are highly known for their violent local effects and such effects have been commonly treated with plant extracts without any scientific validation in rural India. In this investigation Morus alba plant leaf extract has been studied against the Indian Vipera/Daboia russelii venom induced local and systemic effects. The extract completely abolished the in vitro proteolytic and hyaluronolytic activities of the venom. Edema, hemorrhage and myonecrotic activities were also neutralized efficiently. In addition, the extract partially inhibited the pro-coagulant activity and completely abolished the degradation of Aalpha chain of human fibrinogen. Thus, the extract processes potent antisnake venom property, especially against the local and systemic effects of Daboia russelii venom.

  13. UV-B induced changes in the secondary metabolites of Morus alba L. leaves.

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    Gu, Xi-Da; Sun, Ming-Yao; Zhang, Lin; Fu, Hong-Wei; Cui, Lei; Chen, Run-Ze; Zhang, Da-Wei; Tian, Jing-Kui

    2010-04-27

    Ultraviolet-B (UV-B) radiation is harmful to plants and human beings. Many secondary metabolites, like flavonoids, alkaloids, and lignin, are UV-B absorbing compounds, which can protect the genetic material of plants. Furthermore, they are active components of herbal drugs. UV-B radiation can activate the self-protective secondary metabolism system. The results of this paper provide a method to induce bioactive secondary metabolites from mulberry leaves (Morus alba L.) by UV-B irradiation in vitro. Five significantly different chromatographic peaks were found by HPLC fingerprint after induction, from which two active compounds were identified: One was chalcomoracin, a natural Diels-Alder type adduct with antibacterial activity; the other one was moracin N, which is a precursor of chalcomoracin. Their contents were 0.818 mg/g and 0.352 mg/g by dry weight, respectively.

  14. UV-B Induced Changes in the Secondary Metabolites of Morus alba L. Leaves

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    Da-Wei Zhang

    2010-04-01

    Full Text Available Ultraviolet-B (UV-B radiation is harmful to plants and human beings. Many secondary metabolites, like flavonoids, alkaloids, and lignin, are UV-B absorbing compounds, which can protect the genetic material of plants. Furthermore, they are active components of herbal drugs. UV-B radiation can activate the self-protective secondary metabolism system. The results of this paper provide a method to induce bioactive secondary metabolites from mulberry leaves (Morus alba L. by UV-B irradiation in vitro. Five significantly different chromatographic peaks were found by HPLC fingerprint after induction, from which two active compounds were identified: One was chalcomoracin, a natural Diels-Alder type adduct with antibacterial activity; the other one was moracin N, which is a precursor of chalcomoracin. Their contents were 0.818 mg/g and 0.352 mg/g by dry weight, respectively.

  15. Industrial dust sulphate and its effects on biochemical and morphological characteristics of Morus (Morus alba) plant in NCR Delhi.

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    Gupta, Gyan Prakash; Singh, Sudha; Kumar, Bablu; Kulshrestha, U C

    2015-03-01

    Abundance of CaCO3 rich soil dust is a typical feature of atmospheric environment in the Indian region. During prevailing dry weather conditions, dustfall is deposited onto the foliar surfaces of plant affecting their morphology, stomata and the levels of biochemical constituents. This study reports the chemical characteristics of dustfall, its effect on foliar morphology and biochemical constituents of a medicinal plant (Morus alba) at two sites which are differentiated on the basis of landuse pattern, viz., (i) residential, Jawaharlal Nehru University (JNU), and (ii) industrial, Sahibabad (SB), located in the National Capital Region (NCR) of Delhi. Dustfall was characterized for major anions (F(-), Cl(-), NO3 (-) and SO4 (--)) and cations (Na(+), NH4 (+), K(+), Mg(++) and Ca(++)). Biochemical parameters such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, proline and ascorbic acid were determined in foliar samples. The results showed that the dustfall fluxes of all the major ions were found to be higher at the industrial site (SB) as compared to the residential site (JNU). Foliar analysis revealed that the levels of biochemical parameters were more affected at SB site due to higher levels of dust SO4 (--) contributed by various anthropogenic sources resulting in more stressful conditions affecting the biochemistry of the plant. The possible entry pathways for dust SO4 (--) into foliar cells are also discussed in the paper. It was noticed that the deposition of urban dust was responsible for the damage of trichome, epidermis, cuticle and stomatal guard cells significantly affecting foliar morphology. SB exhibited more damage to these morphological parts suggesting that industrial dust is harmful to the plants.

  16. Comportamiento morfoagronómico de variedades de morera (Morus alba L. durante el establecimiento Morphoagronomic performance of mulberry (Morus alba L. varieties during the establishment

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    Gertrudis Pentón

    2007-09-01

    Full Text Available En áreas de la EEPF "Indio Hatuey" se realizó un estudio con el objetivo de evaluar el comportamiento morfoagronómico de cuatro variedades de morera (Morus alba L. (Criolla, Indonesia, Cubana y Tigreada, durante la etapa de establecimiento de plantas trasplantadas. El material vegetativo (posturas fue aviverado durante 120 días. La dinámica de emergencia de las yemas fue significativamente superior en las variedades Indonesia, Tigreada y Cubana con respecto a la Criolla. La variedad Tigreada alcanzó la mayor tasa promedio de desarrollo de nuevas ramas (0,25 ramas/día, aunque sin diferencias significativas respecto a las restantes variedades. La longitud de las ramas tendió a ser superior en la var. Tigreada (0,89 cm promedio por día, seguida por las variedades Indonesia, Cubana y Criolla (0,77; 0,73 y 0,63 cm/día, respectivamente. Sin embargo, el grosor de las ramas fue mayor en las variedades Cubana y Criolla. La variedad Indonesia se caracterizó por un significativo crecimiento en altura y grosor de la base del tallo principal (154 cm y 14,6 mm respecto al resto de las variedades. Todo ello tuvo una repercusión marcada en la producción de hojas, en lo cual se destacó la variedad Tigreada; le siguieron, aunque con diferencias significativas entre ellas, las variedades Indonesia, Cubana y Criolla. Se concluye que Tigreada e Indonesia resultaron ser las variedades de mejor comportamiento morfoagronómico durante el establecimiento; la primera se caracterizó por un alto potencial de producción de hojas y la segunda por una notable capacidad de acumulación de tejido leñoso.A study was carried out in areas of the EEPF "Indio Hatuey" in order to evaluate the morphoagronomic performance of four mulberry (Morus alba L. varieties (Criolla, Indonesia, Cubana and Tigreada, during the establishment stage of transplanted plants. The vegetative material (seedlings was placed in a nursery for 120 days. The bud emergence dynamics was

  17. Allelopathic influence of aqueous extracts from the leaves of Morus alba L. on seed germination and seedling growth of Cucumis sativus L. and Sinapsis alba L.

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    Katarzyna Możdżeń

    2014-04-01

    Full Text Available The aim of the present study was to elucidate impact of the aqueous extracts from leaves of Morus alba L. on germination, growth and photosynthetic activity of Cucumis sativus L. and Sinapis alba L. Plants were grown for 21 days at the temperature 25°C (day and 18°C (night, within 12/12 hours photoperiod, light intensity 150 μmol·m-2·s-1 and relative humidity 60-70% (day/night. Our experiments proved that allelopathic compounds in aqueous extracts of the leaves M. alba at high concentrations, reduce power and energy of germination. Biometric analysis of seedlings and adult plants grown showed that allelopathic substances have stimulating or inhibiting function depending on the stage of treatment. Moreover, they cause changes in chlorophyll contents and activity of photosystem II (PS II.

  18. N-containing sugars from Morus alba and their glycosidase inhibitory activities.

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    Asano, N; Oseki, K; Tomioka, E; Kizu, H; Matsui, K

    1994-06-17

    The reexamination of N-containing sugars from the roots of Morus alba by improved purification procedures led to the isolation of eighteen N-containing sugars, including seven that were isolated from the leaves of Morus bombycis. These N-containing sugars are 1-deoxynojirimycin (1), N-methyl-1-deoxynojirimycin (2), fagomine (3), 3-epi-fagomine (4), 1,4-dideoxy-1,4-imino-D-arabinitol (5), 1,4-dideoxy-1,4-imino-D-ribitol (6), calystegin B2 (1 alpha,2 beta,3 alpha,4 beta-tetrahydroxy-nor-tropane, 7), calystegin C1 (1 alpha,2 beta,3 alpha,4 beta,6 alpha-pentahydroxy-nor-tropane, 8), 1,4-dideoxy-1,4-imino-(2-O-beta-D-glucopyranosyl)-D-arabinitol (9), and nine glycosides of 1. These glycosides consist of 2-O- and 6-O-alpha-D-galactopyranosyl-1-deoxynojirimycins (10 and 11, respectively), 2-O-, 3-O- and 4-O-alpha-D-glucopyranosyl-1-deoxynojirimycins (12, 13, and 14, respectively), and 2-O-, 3-O-, 4-O- and 6-O-beta-D-glucopyranosyl-1-deoxynojirimycins (15, 16, 17, and 18, respectively). Compound 4 is a new member of polyhydroxylated piperidine alkaloids, and the isolation of 6 is the first report of its natural occurrence. It has recently been found that the polyhydroxy-nor-tropane alkaloids possess potent glycosidase inhibitory activities. Calystegin A3 is the trihydroxy-nor-tropane, and calystegins B1 and B2 are the tetrahydroxy-nor-tropane. Calystegin C1, a new member of calystegins, is the first naturally occurring pentahydroxy-nor-tropane alkaloid. The inhibitory activities of these compounds were investigated against rat digestive glycosidases and various commercially available glycosidases.

  19. Atividade Antimicrobiana e Citotoxicidade do extrato bruto obtido de Morus Alba L. (Moraceae

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    Camila Bugnotto Pereira

    2012-01-01

    Full Text Available O presente trabalho avaliou a atividade antimicrobiana in vitro do extrato hidroetanólico e frações hexânica, clorofórmica, acetato de etila e butanólica das folhas de Morus alba L. A concentração inibitória mínima (CIM foi determinada frente à Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Aspergillus fumigatus e Prothoteca zophii. As frações que apresentaram melhores respostas para a atividade antimicrobiana foram acetato de etila e clorofórmica com CIM de 256 µg/mL. Não foi possível detectar atividade antimicrobiana para Aspergillus fumigatus em nenhuma das concentrações testadas. A citotoxidade do extrato hidroetanólico foi avaliada através de culturas de células de ovário de hamster chinês (CHO e células do tecido conectivo de camundongo (NCTC clone 929, determinando o índice de citotoxidade (IC50. O IC50 foi de 0,34 mg/mL para as células CHO e 3,24 mg/mL para as células NCTC 929. De modo geral, as frações acetato de etila e clorofórmica das folhas de M. alba L. apresentaram moderada atividade antimicrobiana e o extrato bruto demonstrou ação citotóxica in vitro frente as células CHO e NCTC 929.

  20. Analysis and characterisation of phytochemicals in mulberry (Morus alba L.) fruits grown in Vojvodina, North Serbia.

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    Natić, Maja M; Dabić, Dragana Č; Papetti, Adele; Fotirić Akšić, Milica M; Ognjanov, Vladislav; Ljubojević, Mirjana; Tešić, Živoslav Lj

    2015-03-15

    In this study, the polyphenolic profile of 11 Morus alba fruits grown in the Vojvodina region was investigated. Ultra high performance liquid chromatography (UHPLC) coupled with Linear Trap Quadrupole and OrbiTrap mass analyzer, and UHPLC coupled with a diode array detector and a triple-quadrupole mass spectrometer were used for the identification and quantification of the polyphenols, respectively. A total of 14 hydroxycinnamic acid esters, 13 flavonol glycosides, and 14 anthocyanins were identified in the extracts with different distributions and contents according to the sampling. The total phenolic content ranged from 43.84 to 326.29 mg GAE/100g frozen fruit. The radical scavenging capacity (50.18-86.79%), metal chelating ability (0.21-8.15%), ferric ion reducing power (0.03-38.45 μM ascorbic acid) and superoxide anion radical scavenging activity (16.53-62.83%) were assessed. The findings indicated that mulberry polyphenolics may act as potent superoxide anion radical scavengers and reducing agents.

  1. Antiosteoporotic Effect of Combined Extract of Morus alba and Polygonum odoratum

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    Sudarat Sungkamanee

    2014-01-01

    Full Text Available Due to the limitation of osteoporosis therapy, the alternative therapies from natural sources have been considered. In this study, we aimed to determine the antiosteoporotic effect of the combined extract of Morus alba and Polygonum odoratum leaves. Ovariectomized rats, weighing 200–220 g, were orally given the combined extract at doses of 5, 150, and 300 mg·kg−1 BW for 3 months. At the end of study, blood was collected to determine serum osteocalcin, calcium, and alkaline phosphatase level. In addition, tibia bone was isolated to determine bone oxidative stress markers, cortical bone thickness, and density of osteoblast. The combined extract decreased oxidative stress and osteoclast density but increased osteoblast density and cortical thickness. The elevation of serum calcium, alkaline phosphatase, and osteocalcin was also observed. These results suggested the antiosteoporotic effect of the combined extract via the increased growth formation together with the suppression of bone resorption. However, further studies concerning chronic toxicity and the underlying mechanism are required.

  2. Characterization of Melanogenesis Inhibitory Constituents of Morus alba Leaves and Optimization of Extraction Conditions Using Response Surface Methodology

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    Ji Yeon Jeong

    2015-05-01

    Full Text Available Melanin is a natural pigment that plays an important role in the protection of skin, however, hyperpigmentation cause by excessive levels of melatonin is associated with several problems. Therefore, melanogenesis inhibitory natural products have been developed by the cosmetic industry as skin medications. The leaves of Morus alba (Moraceae have been reported to inhibit melanogenesis, therefore, characterization of the melanogenesis inhibitory constituents of M. alba leaves was attempted in this study. Twenty compounds including eight benzofurans, 10 flavonoids, one stilbenoid and one chalcone were isolated from M. alba leaves and these phenolic constituents were shown to significantly inhibit tyrosinase activity and melanin content in B6F10 melanoma cells. To maximize the melanogenesis inhibitory activity and active phenolic contents, optimized M. alba leave extraction conditions were predicted using response surface methodology as a methanol concentration of 85.2%; an extraction temperature of 53.2 °C and an extraction time of 2 h. The tyrosinase inhibition and total phenolic content under optimal conditions were found to be 74.8% inhibition and 24.8 μg GAE/mg extract, which were well-matched with the predicted values of 75.0% inhibition and 23.8 μg GAE/mg extract. These results shall provide useful information about melanogenesis inhibitory constituents and optimized extracts from M. alba leaves as cosmetic therapeutics to reduce skin hyperpigmentation.

  3. Ameliorative effect of Morus alba leaves extract against developmental retinopathy in pups of diabetic and aluminum intoxicated pregnant albino rats

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    Hassan; El-Sayyed; Gamal; Badawy; Sobhy; Hassab; Elnabi; Ibrahim; El-Elaimy; Eman; Al; Shehari

    2015-01-01

    Objective: To investigate the possible ameliorative effect of crude water extract of Morus alba(M. alba) leaves on retinopathy of rat pups maternally subjected to diabetes and/or Al intoxication.Methods: Both control and experimental groups were subjected to certain integrated approaches, namely, biochemical assessments, light microscopic investigation, transmission electron microscopic investigation, single cell gel electrophoresis(comet assay) and determination of DNA fragmentation.Results: The retina of pups of diabetic and/or Al-intoxicated mothers exhibited abnormal alterations in retinal cell layers including retinal pigmented epithelium, photoreceptor inner segment and ganglion cells. Increased incidence of DNA fragmentation and apoptosis were evident in pups of diabetic and/or Al-intoxicated mothers. However, retina of pups maternally received M. alba extract plus diabetes or Al-intoxicated alone or in combination showed marked amelioration. Less degree of ameliorations was seen in retina of pups maternally subjected to combined treatment. Furthermore, application of crude water extract of M.alba resulted in amelioration of the alterations of maternal serum glucose as well as Al concentration.Conclusions: Based on the results of the present study, M. alba extract is effective against experimentally diabetic and Al-induced developmental retinopathy.

  4. Protective Effects of Morus alba Leaves Extract on Ocular Functions of Pups from Diabetic and Hypercholesterolemic Mother Rats

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    H.I.H. El-Sayyad, M.A. El-Sherbiny, M.A. Sobh, A.M. Abou-El-Naga, M.A.N. Ibrahim, S.A. Mousa

    2011-01-01

    Full Text Available Phytotherapy is frequently considered to be less toxic and free from side effects than synthetic drugs. Hence, the present study was designed to investigate the protective use of crude water extract of Morus alba leaves on ocular functions including cataractogenesis, biochemical diabetic and hypercholesterolemic markers, retinal neurotransmitters and retinopathy of rat pups maternally subjected to either diabetes and/or hypercholesterolemia. Application of crude water extract of Morus alba resulted in amelioration of the alterations of maternal serum glucose, LDL, HDL, total cholesterol and creatine phosphokinase activity as well as retinal neurotransmitters including acetylcholine (ACE, adrenaline (AD, nor-adrenaline (NAD, serotonin (5-HT, histamine (HS, dopamine (DA and gamma amino butyric acid (GABA. The retina of pups of either diabetic and/or hypercholesterolemia mothers exhibited massive alterations of retinal neurotransmitters. The alterations of retinal neurotransmitters were correlated with the observed pathological alterations of retinal pigmented epithelium, photoreceptor inner segment and ganglion cells and increased incidence of DNA fragmentation and apoptosis cell death. However, protection with Morus alba extract led to amelioration of the pathological alterations of retinal neurons and estimated neurotransmitters. Furthermore, a striking incidence of cataract was detected in pups of either diabetic and/or hypercholesterolemic mothers. Highest cataractogenesis was observed in pups of combined -treated groups. Our data indicate that experimental maternal diabetes alone or in combination with hypercholesterolemia led to alteration in the ocular structures of their pups, with an increasing incidence of cataract and retinopathy, and the effects of the extract might be attributed to the hypoglycaemic, antihypercholesterolemic and anti-oxidative potential of flavonoids, the major components of the plant extract.

  5. Resultados preliminares del forraje de Morus alba en la alimentación de vacas lecheras

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    M. Milera

    2007-01-01

    Full Text Available El objetivo del presente trabajo consistió en evaluar el potencial del forraje de M. alba con vacas mestizas para la producción de leche. El trabajo se desarrolló en Cuba, la cual se encuentra localizada entre los 19° y 81’ de longitud oeste. La Estación Experimental de Pastos y Forrajes “Indio Hatuey” está ubicada en el municipio de Perico, provincia de Matanzas, sobre un suelo ferralítico rojo, a los 22° 40’ 7’’ de latitud norte y 81° 2’ de longitud oeste, a una altura de 10.91 msnm. La evaluación se hizo con vacas mestizas del cruce Holstein x Cebú, que tenían 54 días de lactancia. El estudio se dividió en dos periodos: en el primero (que duró 53 días se suministró el forraje de morera ad libitum, sin trocear, y se pastoreó de forma restringida en gramíneas mejoradas de secano, sin fertilización; en el segundo periodo, los animales dispusieron de pastoreo de gramíneas mejoradas con un 10% de un área establecida con Leucaena leucocephala y recibieron forraje de morera restringido hasta el 1% del peso vivo; éste tuvo una duración de 87 días. Se determinó la disponibilidad de pasto, la composición bromatológica y el consumo de forraje de Morus alba, así como la producción de leche de las vacas. El forraje de morera presentó altos va- lores de proteína y bajos contenidos de fibra; los consumos en el primer periodo llegaron hasta 2.7% del peso vivo. La producción de leche promedio fue de 10.6 kg/vaca/día durante los 140 días de evaluación y la máxima producción se registró en los primeros 53 días (1 kg/vaca/día. Los resultados demostraron la alta calidad de la morera cuando se utiliza como forraje para la alimentación de vacas mestizas. En las condiciones evaluadas, es posible obtener producciones de 10 litros por animal diariamente, cuando se utiliza el forraje de morera en adición al pasto de especies mejoradas, sin suplementación de concentrados.

  6. Biphasic effects of Morus alba leaves green tea extract on mice in chronic forced swimming model.

    Science.gov (United States)

    Sattayasai, Jintana; Tiamkao, Siriporn; Puapairoj, Prapawadee

    2008-04-01

    In this study, the effects of an aqueous extract of Morus alba leaves green tea (ME) on mouse behaviors (depression, anxiety, climbing activity and thermal response), muscle coordination and muscle strength were studied. Male IRC mice received a single intraperitoneal injection of either the ME, desipramine or diazepam. Thirty minutes after injection, the mice were tested in all experimental models. A significant antidepressant-like effect could be detected in the animals receiving either 100 or 200 mg/kg ME. The effect of 200 mg/kg ME in decreasing the immobility time was comparable to 10 mg/kg desipramine. With higher dose (1000 mg/kg), a significant increase in immobility time could be observed. In the elevated plus maze, no increase in time in the open arm could be observed in mice treated with ME at either 100 or 200 mg/kg. However, high doses of ME (500 or 1000 mg/kg) decreased both time in the open arm and the number of entries in the maze. No change in thermal response could be seen in mice treated with ME at doses up to 500 mg/kg, however, at 1000 mg/kg, the response time to heat was increased significantly. The ME at either 500 or 1000 mg/kg also decreased muscle coordination, strength and climbing activity significantly when compared with the control. This study suggests that ME possesses an antidepressant- without an anxiolytic-like effect, however, at high doses, the extract might show the sedative effect and alter other functions such as muscle strength, animal activity in the maze and pain response.

  7. Influência de genótipos de amoreira (Morus sp. e substratos no peso e características de casulos do bicho-da-seda (Bombyx mori L. Influence of mulberry (Morus sp. genotypes and substrates in weight and characteristics of silkworm cocoons (Bombyx mori L.

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    Odinete Murari

    2001-05-01

    Full Text Available Avaliou-se o efeito de três genótipos de amoreira, Morus sp. (Moraceae: Miura, FM Shima Miura e IZ 56/4, três tipos de esteiras de criação: terra compactada, concreto e tela plástica sobre o peso e algumas características industriais de casulos produzidos pelo bicho-da-seda, Bombyx mori (Lepidoptera. Houve influência das interações de genótipos com esteiras de criação sobre o peso dos casulos produzidos. Com relação às características industriais, os tratamentos que mais se sobressaíram foram: Miura / terra compactada, FM-SM / tela plástica, IZ 56/4 / terra compactada e IZ 56/4 / tela plástica.The effect of three genotypes of mulberry, Morus sp. (Moraceae, namely, Miura, FM Shima Miura and IZ 56/4, and three types of rearing substrate comprising compact soil, concrete and plastic screen were estimated on weight and on certain industrial characteristics of Bombyx mori (Lepidoptera cocoons. Genotype interactions with rearing substrates affected weight of cocoons produced. Analysis showed the best treatments for manufacturer parameters were Miura / compact soil, FM-Shima Miura / plastic screen, IZ 56/4 / compact soil and IZ 56/4 / plastic screen.

  8. Accumulation of Rutin and Betulinic Acid and Expression of Phenylpropanoid and Triterpenoid Biosynthetic Genes in Mulberry (Morus alba L.).

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    Zhao, Shicheng; Park, Chang Ha; Li, Xiaohua; Kim, Yeon Bok; Yang, Jingli; Sung, Gyoo Byung; Park, Nam Il; Kim, Soonok; Park, Sang Un

    2015-09-30

    Mulberry (Morus alba L.) is used in traditional Chinese medicine and is the sole food source of the silkworm. Here, 21 cDNAs encoding phenylpropanoid biosynthetic genes and 21 cDNAs encoding triterpene biosynthetic genes were isolated from mulberry. The expression levels of genes involved in these biosynthetic pathways and the accumulation of rutin, betulin, and betulinic acid, important secondary metabolites, were investigated in different plant organs. Most phenylpropanoid and triterpene biosynthetic genes were highly expressed in leaves and/or fruit, and most genes were downregulated during fruit ripening. The accumulation of rutin was more than fivefold higher in leaves than in other organs, and higher levels of betulin and betulinic acid were found in roots and leaves than in fruit. By comparing the contents of these compounds with gene expression levels, we speculate that MaUGT78D1 and MaLUS play important regulatory roles in the rutin and betulin biosynthetic pathways.

  9. Determinación de antioxidantes enzimáticos en variedades e híbridos de Morus alba Determination of enzymatic antioxidants in Morus alba varieties and hybrids

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    2010-09-01

    Full Text Available En el presente trabajo se evaluó la actividad específica de las enzimas antioxidantes catalasa y guaiacol peroxidasa en extractos frescos de variedades e híbridos de morera (Morus alba, obtenidos a partir de las raíces, los tallos y las hojas. Las muestras de los diferentes órganos en estudio fueron colectadas aleatoriamente, maceradas en nitrógeno líquido, resuspendidas en tampón fosfato de sodio y centrifugadas. El sobrenadante se utilizó para las determinaciones enzimáticas. Se empleó el ANOVA de clasificación simple y la prueba de rangos múltiples de Duncan para la comparación de medias (PIn this work the specific activity of the antioxidant enzymes catalase and guaiacol peroxidase was evaluated in fresh extracts of mulberry (Morus alba varieties and hybrids, obtained from the roots, stems and leaves. The samples of the different organs under study were randomly collected, macerated in liquid nitrogen, resuspended in sodium phosphate buffer and centrifuged. The supernatant was used for the enzymatic determinations. The simple classification ANOVA and Duncan´s multiple range test were used for mean comparison (P<0,05. The highest values of specific catalase and peroxidase activity were found, in general, in the leaves, followed by stems and roots. The varieties and hybrids with higher specific activity for both enzymes were: Tigreada, Criolla, IZ 15-9 and IZ 64. The results showed the protective antioxidant role of the extracts, mainly from leaves, from the important functions played by these enzymatic systems in the elimination of reactive oxygen species. The potential of this species for animal nutrition and health is corroborated, which gives it more value as a multipurpose forage plant. It is recommended to continue making other determinations of compounds with antioxidant activities and studying the catalase and peroxidase enzymatic systems in multipurpose species, such as M. alba, used in animal feeding and traditional

  10. Morus alba leaf extract mediates neuroprotection against glyphosate-induced toxicity and biochemical alterations in the brain.

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    Rebai, Olfa; Belkhir, Manel; Boujelben, Adnen; Fattouch, Sami; Amri, Mohamed

    2017-04-01

    Recent studies demonstrate that glyphosate exposure is associated with oxidative stress and some neurological disorders such as Parkinson's pathology. Therefore, phytochemicals, in particular phenolic compounds, have attracted increasing attention as potential agents for neuroprotection. In the present study, we investigate the impact of glyphosate on the rat brain following i.p. injection and the possible molecular target of neuroprotective activity of the phenolic fraction from Morus alba leaf extract (MALE) and its ability to reduce oxidative damage in the brain. Wistar rats from 180 to 240 g were i.p. treated with a single dose of glyphosate (100 mg kg(-1) b.w.) or MALE (100 μg mL(-1) kg(-1) b.w.) for 2 weeks. Brain homogenates were used to evaluate neurotoxicity induced by the pesticide. For this, biochemical parameters were measured. Data shows that MALE regulated oxidative stress and counteracted glyphosate-induced deleterious effects and oxidative damage in the brain, as it abrogated LDH, protein carbonyls, and malonyldialdehyde. MALE also appears to be able to scavenge H2O2 levels, maintain iron and Ca(2+) homeostasis, and increase SOD activity. Thus, in vivo results showed that mulberry leaf extract is a potent protector against glyphosate-induced toxicity, and its protective effect could result from synergism or antagonism between the various bioactive phenolic compounds in the acetonic fraction from M. alba leaf extract.

  11. Genetic diversity analyses of Lasiodiplodia theobromae on Morus alba and Agave sisalana based on RAPD and ISSR molecular markers

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    Hong-hui Xie

    2016-10-01

    Full Text Available Genetic diversity of 23 Lasiodiplodia theobromae isolates on Morus alba and 6 isolates on Agave sisalana in Guangxi province, China, was studied by using random amplified polymorphic DNA and inter-simple sequence repeat molecular markers. Results of two molecular markers showed that the average percentage of polymorphic loci of all isolates was more than 93%. Both dendrograms of two molecular markers showed obvious relationship between groups and the geographical locations where those strains were collected, among which, the 23 isolates on M. alba were divided into 4 populations and the 6 isolates on A. sisalana were separated as a independent population. The average genetic identity and genetic distance of 5 populations were 0.7215, 0.3284 and 0.7915, 0.2347, respectively, which indicated that the genetic identity was high and the genetic distance was short in the 5 populations. Average value of the gene diversity index (H and the Shannon’s information index (I of 29 isolates were significantly higher than 5 populations which showed that genetic diversity of those isolates was richer than the populations and the degree of genetic differentiation of the isolates was higher. The Gst and Nm of 29 isolates were 0.4411, 0.6335 and 0.4756, 0.5513, respectively, which showed that the genetic diversity was rich in those isolates.

  12. In vitro antibacterial activity of ethanolic extract of Morus alba leaf against periodontal pathogens

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    Shilpa Gunjal

    2015-01-01

    Results: P. gingivalis was the most sensitive organism against the M. alba extract with an MIC value of 1.95 mg/ml; while T. forsythia and P. gingivalis both were most sensitive organisms against chlorhexidine gluconate with MIC values of 0.00781 mg/ml. Conclusion: M. alba possess good antibacterial activity against A. actinomycetemcomitans, P. gingivalis and T. forsythia and thus would be beneficial for the prevention and treatment of periodontal disease. However, chlorhexidine gluconate was found to be more effective when compared to M. alba.

  13. Pollution trends using bark of morus alba in the cities of buenos aires and mendoza (Argentina Tendências de poluição usando cascas de morus alba nas cidades de buenos aires e mendoza (Argentina

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    Patricia Perelman

    2010-06-01

    Full Text Available A comparative study of elements deposited on tree bark was carried out for urban and periurban areas of two of the most important cities in Argentina. The content of Fe, Mg, Al, Mn, Zn, Pb, Ba, Cr, Hg, Cu, Ni, Cd and Sb was determined by inductively coupled plasma atomic emission spectrometry (ICP-OES in Morus alba tree bark collected in the cities of Buenos Aires and Mendoza. The main air pollutants detected in the Buenos Aires urban area were Ba, Cr, Cu and Ni and indicate significative difference from the Mendoza urban and periurban areas. Significantly, higher concentrations of Zn, Ba, Cr and Cu were recorded in the periurban area of the city of Buenos Aires than in Mendoza. Bark samples were strongly influenced by dust and show Al, Fe, Mg and other element accumulations that indicate that soil particles were carried out by wind. Elements like Ba and Zn, commonly linked to traffic emissions, showed the highest concentrations in the Buenos Aires metropolitan area, possibly due to more intensive vehicular traffic. Our results indicated that intensity of vehicular traffic and not city structure is responsible for air pollution.Um estudo comparativo dos elementos depositados nas cascas das árvores foi realizado nas áreas urbanas e na periferia de duas cidades das mais importantes da Argentina. Os índices do Fe, Mg, Al, Mn, Zn, Pb, Ba, Cr, Cu, Ni e do Sb foram determinados pela espectrometria da emissão atômica com plasma acoplado indutivamente (ICP-OES nas cascas da árvore Morus alba, coletados nas cidades de Buenos Aires e Mendoza. Os principais elementos da contaminação do ar detectadas na área urbana de Buenos Aires foram Ba, Cr, Cu e Ni, que indicaram diferenças significativas nas áreas urbanas e na periferia de Mendoza. Uma concentração do Zn, Ba, Cr e do Cu foi achada na periferia da cidade de Buenos Aires, significativamente mais elevada do que em Mendoza. As amostras da casca foram influenciadas fortemente pela poeira e

  14. Efecto del forraje de Morus alba en los indicadores productivos y de salud de bovinos jóvenes en pastoreo Effect of Morus alba forage on the productive and health indicators of grazing calves

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    Mildrey Soca

    2010-12-01

    Full Text Available Con el objetivo de evaluar las potencialidades de la morera (Morus alba var. Cubana para la suplementación de bovinos jóvenes en pastoreo y sus efectos en la productividad y la salud de los animales, se desarrolló esta investigación en la EEPF "Indio Hatuey" durante la época lluviosa, con dos tratamientos: el grupo experimental recibía 6 kg de morera fresca troceada y 500 g de concentrado/animal/día (A; mientras que en el grupo control la suplementación era de 1 kg de concentrado/animal/día y heno de pangola a voluntad (B. Se evalúo la composición bromatológica y fitoquímica de la morera, el peso vivo, la ganancia media diaria (GMD y el conteo fecal de huevos (CFH de nemátodos gastrointestinales en los animales. Se observaron diferencias significativas (PIn order to evaluate the potential of mulberry (Morus alba var. Cubana for the supplementation of young grazing cattle and its effects on the productivity and health of the animals, this study was conducted at the EEPF "Indio Hatuey" during the rainy season, with two treatments: the experimental group received 6 kg of fresh chopped mulberry and 500 g of concentrate/animal/day (A; while in the control group supplementation was 1 kg of concentrate/animal/day and pangola grass hay ad libitum (B. The bromatological and phytochemical composition of mulberry, live weight, mean daily gain (MDG and fecal egg count (FEC of gastrointestinal nematodes in the animals were evaluated. Significant differences (P<0,001 were observed in the live weight between treatments (186,06 vs 169,01 kg. Likewise, a significant effect (P<0,05 of the MDG was detected, with values higher than 600 g in the supplemented animals. On the other hand, the FEC showed significant differences (P<0,01 since the second month of evaluation, with stable performance and values lower than 100 epg in the experimental group. Seven groups of secondary metabolites were found; the most representative ones were phenols, tripertene

  15. Appetite Suppression and Antiobesity Effect of a Botanical Composition Composed of Morus alba, Yerba mate, and Magnolia officinalis

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    Mesfin Yimam

    2016-01-01

    Full Text Available Background. Obesity and its comorbidities continue to challenge the world at an alarming rate. Although the long term solution lies on lifestyle changes in the form of dieting and exercising, drug, medical food, or dietary supplement interventions are required for those who are already obese. Here we describe a standardized blend composed of extracts from three medicinal plants: Morus alba, Yerba mate, and Magnolia officinalis for appetite suppression and metabolic disorders management. Method. Extracts were standardized to yield a composition designated as UP601. Appetite suppression activity was tested in acute feed intake rat model. Efficacy was evaluated in C57BL/6J mouse models treated with oral doses of 1.3 g/kg/day for 7 weeks. Orlistat at 40 mg/kg/day was used as a positive control. Body compositions of mice were assessed using a dual energy X-ray absorptiometry (DEXA. ELISA was done for insulin, leptin, and ghrelin level quantitation. Nonalcoholic steatohepatitis (NASH scoring was conducted. Results. Marked acute hypophagia with 81.8, 75.3, 43.9, and 30.9% reductions in food intake at 2, 4, 6, and 24 hours were observed for UP601. Decreases in body weight gain (21.5% compared to the HFD at weeks 7 and 8.2% compared to baseline and calorie intake (40.5% for the first week were observed. 75.9% and 46.8% reductions in insulin and leptin, respectively, 4.2-fold increase in ghrelin level, and reductions of 18.6% in cholesterol and 59% in low-density lipoprotein were documented. A percentage body fat of 18.9%, 47.8%, 46.1%, and 30.4% was found for mice treated with normal control, HFD, Orlistat, and UP601, respectively. 59.3% less mesenteric fat pad and improved NASH scores were observed for UP601. Conclusion. UP601, a standardized botanical composition from Morus alba, Yerba mate, and Magnolia officinalis could be used as a natural alternative for appetite suppression, maintaining healthy body weight and metabolism management.

  16. Appetite Suppression and Antiobesity Effect of a Botanical Composition Composed of Morus alba, Yerba mate, and Magnolia officinalis

    Science.gov (United States)

    Jiao, Ping; Hong, Mei; Brownell, Lidia; Lee, Young-Chul; Hyun, Eu-Jin; Kim, Hyun-Jin; Kim, Tae-Woo; Nam, Jeong-Bum; Kim, Mi-Ran; Jia, Qi

    2016-01-01

    Background. Obesity and its comorbidities continue to challenge the world at an alarming rate. Although the long term solution lies on lifestyle changes in the form of dieting and exercising, drug, medical food, or dietary supplement interventions are required for those who are already obese. Here we describe a standardized blend composed of extracts from three medicinal plants: Morus alba, Yerba mate, and Magnolia officinalis for appetite suppression and metabolic disorders management. Method. Extracts were standardized to yield a composition designated as UP601. Appetite suppression activity was tested in acute feed intake rat model. Efficacy was evaluated in C57BL/6J mouse models treated with oral doses of 1.3 g/kg/day for 7 weeks. Orlistat at 40 mg/kg/day was used as a positive control. Body compositions of mice were assessed using a dual energy X-ray absorptiometry (DEXA). ELISA was done for insulin, leptin, and ghrelin level quantitation. Nonalcoholic steatohepatitis (NASH) scoring was conducted. Results. Marked acute hypophagia with 81.8, 75.3, 43.9, and 30.9% reductions in food intake at 2, 4, 6, and 24 hours were observed for UP601. Decreases in body weight gain (21.5% compared to the HFD at weeks 7 and 8.2% compared to baseline) and calorie intake (40.5% for the first week) were observed. 75.9% and 46.8% reductions in insulin and leptin, respectively, 4.2-fold increase in ghrelin level, and reductions of 18.6% in cholesterol and 59% in low-density lipoprotein were documented. A percentage body fat of 18.9%, 47.8%, 46.1%, and 30.4% was found for mice treated with normal control, HFD, Orlistat, and UP601, respectively. 59.3% less mesenteric fat pad and improved NASH scores were observed for UP601. Conclusion. UP601, a standardized botanical composition from Morus alba, Yerba mate, and Magnolia officinalis could be used as a natural alternative for appetite suppression, maintaining healthy body weight and metabolism management. PMID:27699065

  17. Effects of drying method and particle size on the antioxidant properties of leaves and teas of Morus alba, Lagerstroemia speciosa and Thunbergia laurifolia

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    Eric Wei Chiang Chan

    2012-01-01

    Full Text Available Antioxidant properties (AOP of leaves and teas of Morus alba L., Lagerstroemia speciosa (L. Pers. and Thunbergia laurifolia Lindl. as affected by microwave, oven and freeze drying were assessed. Total phenolic content (TPC, radical scavenging ability expressed as ascorbic acid equivalent capacity (AEAC and ferric reducing power (FRP were screened using the Folin-Ciocalteu, 2,2-diphenyl-1-picrylhydrazyl (DPPH and potassium ferricyanide assays, respectively. The effects of particle size were also investigated. Microwave drying resulted in enhanced AOP in M. alba and T. laurifolia. Oven drying resulted in declined AOP in T. laurifolia with M. alba and L. speciosa relatively unchanged. Significant increase in AOP was observed in freeze-dried leaves of M. alba with L. speciosa and T. laurifolia showing no change or slight increase. TPC, AEAC and FRP of ground microwave-, oven- and freeze-dried leaves of M. alba extracted with 50% methanol were significantly higher than shredded leaves. For tea infusions extracted with hot water, three categories were recognised, i.e. species with shredded leaves yielding stronger AOP (M. alba, species with ground leaves yielding stronger AOP (L. speciosa, and species with ground and shredded leaves yielding comparable AOP (T. laurifolia.

  18. Antidepressant-Like Effects of the Ethyl Acetate Soluble Fraction of the Root Bark of Morus alba on the Immobility Behavior of Rats in the Forced Swim Test

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    Dong Wook Lim

    2014-06-01

    Full Text Available In this study, the antidepressant-like effects of Morus alba fractions in rats were investigated in the forced swim test (FST. Male Wistar rats (9-week-old were administered orally the M. alba ethyl acetate (EtOAc 30 and 100 mg/kg and M. alba n-butanol fractions (n-BuOH 30 and 100 mg/kg every day for 7 consecutive days. On day 7, 1 h after the final administration of the fractions, the rats were exposed to the FST. M. alba EtOAc fraction at the dose of 100 mg/kg induced a decrease in immobility behavior (p < 0.01 with a concomitant increase in both climbing (p < 0.05 and swimming (p < 0.05 behaviors when compared with the control group, and M. alba EtOAc fraction at the dose of 100 mg/kg decreased the hypothalamic-pituitary-adrenal (HPA axis response to the stress, as indicated by an attenuated corticosterone response and decreased c-fos immunoreactivity in the hippocampal and hypothalamic paraventricular nucleus (PVN region. These findings demonstrated that M. alba EtOAc fraction have beneficial effects on depressive behaviors and restore both altered c-fos expression and HPA activity.

  19. Antidepressant-like effects of the ethyl acetate soluble fraction of the root bark of Morus alba on the immobility behavior of rats in the forced swim test.

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    Lim, Dong Wook; Kim, Yun Tai; Park, Ji-Hae; Baek, Nam-In; Han, Daeseok

    2014-06-12

    In this study, the antidepressant-like effects of Morus alba fractions in rats were investigated in the forced swim test (FST). Male Wistar rats (9-week-old) were administered orally the M. alba ethyl acetate (EtOAc 30 and 100 mg/kg) and M. alba n-butanol fractions (n-BuOH 30 and 100 mg/kg) every day for 7 consecutive days. On day 7, 1 h after the final administration of the fractions, the rats were exposed to the FST. M. alba EtOAc fraction at the dose of 100 mg/kg induced a decrease in immobility behavior (p swimming (p < 0.05) behaviors when compared with the control group, and M. alba EtOAc fraction at the dose of 100 mg/kg decreased the hypothalamic-pituitary-adrenal (HPA) axis response to the stress, as indicated by an attenuated corticosterone response and decreased c-fos immunoreactivity in the hippocampal and hypothalamic paraventricular nucleus (PVN) region. These findings demonstrated that M. alba EtOAc fraction have beneficial effects on depressive behaviors and restore both altered c-fos expression and HPA activity.

  20. Morus alba o Hibiscus rosa-sinensis como sustituto parcial de soya en dietas integrales para conejos

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    P. E. Lara

    2012-01-01

    Full Text Available Los conejos pueden aprovechar los nutrientes contenidos en los forrajes y subproductos agrícolas eficientemente gracias a la fermentación cecal y a la cecotrofía. Los objetivos del presente trabajo fueron: evaluar la tasa de crecimiento y el rendimiento de la canal en conejos alimentados con minibloques de harina de morera (Morus alba o tulipán (Hibiscus rosa-sinensis en sustitución parcial de pasta de soya; y determinar el consumo y digestibilidad de la dieta. Para esto, se utilizaron 18 conejos de 30 días de edad, distribuidos al azar en tres dietas experimentales (n=6, ac (alimento convencional, mbm (minibloque con 30% de morera y, mbt (minibloque con 27% de tulipán, durante nueve semanas. La ganancia diaria y el rendimiento en canal fue mejor (p0.10 en los tres grupos. Es factible sustituir parcialmente la proteína de la soya por harina de hojas de morera o tulipán, aunque presentan una menor respuesta productiva con respecto a la alimentación convencional.

  1. The isolation and the characterization of two polysaccharides from the branch bark of mulberry (Morus alba L.).

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    Qiu, Fan; He, Tian-Zhen; Zhang, Yu-Qing

    2016-07-01

    Two water-soluble polysaccharides termed MBBP-1 and MBBP-2 were isolated from the branches of the mulberry tree (Morus alba L.) using hot water extraction and purified on Anion-exchange DEAE52-cellulose and Sephadex G-100 column. MBBP-1 was shown to be composed of rhamnose, xylose, arabinose, mannose, glucose and galactose in the molar ratio of 4.53:2.49:4.38:4.67:17.85:5.88. MBBP-2 was composed of rhamnose, xylose, arabinose, mannose, glucose, galactose and galacturonic acid in the molar ratio of 26.85:13.8:3.14:4.4:6.1:3.19:4.9. Their structural characteristics were further investigated by FI-IR spectroscopy, Smith degradation, methylation analysis and NMR spectroscopy. Based on the data obtained, MBBP-1 had a backbone mainly consisting of (1 → 3)-linked glucose. MBBP-2 had a backbone mainly consisting of (1 → 3)-linked rhamnose and (1 → 2, 4)-linked xylose. Antioxidant assays indicated that antioxidant activities of MBBP-2 were significantly stronger than those of MBBP-1, and this was likely in relation to the different content of 8.2 % galacturonic acid in MBBP-2.

  2. White Mulberry (Morus alba Foliage Methanolic Extract Can Alleviate Aeromonas hydrophila Infection in African Catfish (Clarias gariepinus

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    Atefeh Sheikhlar

    2014-01-01

    Full Text Available Two experiments were simultaneously conducted with Morus alba (white mulberry foliage extract (MFE as a growth promoter and treatment of Aeromonas hydrophila infection in separate 60 and 30 days trail (Experiments 1 and 2, resp. in African catfish (Clarias gariepinus. In Experiment 1, four diets, control and control supplemented with 2, 5, or 7 g MFE/kg dry matter (DM of diet, were used. In Experiment 2, fish were intraperitoneally infected with Aeromonas hydrophila and fed the same diets as experiment 1 plus additional two diets with or without antibiotic. Results of experiment 1 showed that growth was unaffected by dietary levels of MFE. Treatments with the inclusion of MFE at the levels of 5 and 7 g/Kg DM had no mortality. Red blood cells (RBC, albumin, and total protein were all higher for the treatments fed MFE (5 and 7 g/Kg DM. Results of experiment 2 showed RBC, hemoglobin, hematocrit, globulin, albumin, and total protein improved with the increase in MFE in the infected fish. The dietary MFE at the level of 7 g/kg DM reduced mortality rate. In conclusion, MFE at the level of 7 g/kg DM could be a valuable dietary supplement to cure the infected fish.

  3. White mulberry (Morus alba) foliage methanolic extract can alleviate Aeromonas hydrophila infection in African catfish (Clarias gariepinus).

    Science.gov (United States)

    Sheikhlar, Atefeh; Alimon, Abd Razk; Daud, Hassan; Saad, Chee R; Webster, Carl D; Meng, Goh Yong; Ebrahimi, Mahdi

    2014-01-01

    Two experiments were simultaneously conducted with Morus alba (white mulberry) foliage extract (MFE) as a growth promoter and treatment of Aeromonas hydrophila infection in separate 60 and 30 days trail (Experiments 1 and 2, resp.) in African catfish (Clarias gariepinus). In Experiment 1, four diets, control and control supplemented with 2, 5, or 7 g MFE/kg dry matter (DM) of diet, were used. In Experiment 2, fish were intraperitoneally infected with Aeromonas hydrophila and fed the same diets as experiment 1 plus additional two diets with or without antibiotic. Results of experiment 1 showed that growth was unaffected by dietary levels of MFE. Treatments with the inclusion of MFE at the levels of 5 and 7 g/Kg DM had no mortality. Red blood cells (RBC), albumin, and total protein were all higher for the treatments fed MFE (5 and 7 g/Kg DM). Results of experiment 2 showed RBC, hemoglobin, hematocrit, globulin, albumin, and total protein improved with the increase in MFE in the infected fish. The dietary MFE at the level of 7 g/kg DM reduced mortality rate. In conclusion, MFE at the level of 7 g/kg DM could be a valuable dietary supplement to cure the infected fish.

  4. Preparation of the Branch Bark Ethanol Extract in Mulberry Morus alba, Its Antioxidation, and Antihyperglycemic Activity In Vivo

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    Shu Wang

    2014-01-01

    Full Text Available The biological activities of the branch bark ethanol extract (BBEE in the mulberry Morus alba L. were investigated. The determination of active component showed that the flavonoids, phenols, and saccharides are the major components of the ethanol extract. The BBEE had a good scavenging activity of the 1,1-diphenyl-2-picrylhydrazyl (DPPH radical with around 100 μg/mL of IC50 value. In vitro assay revealed that the BBEE strongly inhibited both α-glucosidase and sucrase activities whose IC50 values were 8.0 and 0.24 μg/mL, respectively. The kinetic analysis showed that the BBEE as a kind of α-glucosidase inhibitor characterized a competitive inhibition activity. Furthermore, the carbohydrate tolerance of the normal mice was obviously enhanced at 0.5 h (P<0.05 and 1.0 h (P<0.05 after the BBEE intragastric administration as compared to negative control. At 0.5, 1.0, 1.5, and 2.0 h after the intragastric administration with starch, the postprandial hyperglycemia of the type 2 diabetic mice can be significantly decreased (P<0.01 by supplying various concentrations of the BBEE (10–40 mg/kg body weight. Therefore, the BBEE could effectively inhibit the postprandial hyperglycemia as a novel α-glucosidase activity inhibitor for the diabetic therapy.

  5. Evaluation of Toxicity and Antimicrobial Activity of an Ethanolic Extract from Leaves of Morus alba L. (Moraceae

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    Alisson Macário de Oliveira

    2015-01-01

    Full Text Available This work evaluated an ethanolic extract from Morus alba leaves for toxicity to Artemia salina, oral toxicity to mice, and antimicrobial activity. Phytochemical analysis revealed the presence of coumarins, flavonoids, tannins, and triterpenes in the extract, which did not show toxicity to A. salina nauplii. No mortality and behavioral alterations were detected for mice treated with the extract (300 and 2000 mg/kg b.w. for 14 days. However, animals that received the highest dose showed reduced MCV and MCHC as well as increased serum alkaline phosphatase activity. In treatments with the extract at both 300 and 2000 mg/kg, there was a reduction in number of leukocytes, with decrease in percentage of lymphocytes and increase in proportion of segmented cells. Histopathological analysis of organs from mice treated with the extract at 2000 mg/kg revealed turgidity of contorted tubules in kidneys, presence of leukocyte infiltration around the liver centrilobular vein, and high dispersion of the spleen white pulp. The extract showed antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Candida krusei, Candida tropicalis, and Aspergillus flavus. In conclusion, the extract contains antimicrobial agents and was not lethal for mice when ingested; however, its use requires caution because it promoted biochemical, hematological, and histopathological alterations.

  6. Evaluation of Toxicity and Antimicrobial Activity of an Ethanolic Extract from Leaves of Morus alba L. (Moraceae)

    Science.gov (United States)

    de Oliveira, Alisson Macário; Mesquita, Matheus da Silva; da Silva, Gabriela Cavalcante; de Oliveira Lima, Edeltrudes; de Medeiros, Paloma Lys; Paiva, Patrícia Maria Guedes; de Souza, Ivone Antônia; Napoleão, Thiago Henrique

    2015-01-01

    This work evaluated an ethanolic extract from Morus alba leaves for toxicity to Artemia salina, oral toxicity to mice, and antimicrobial activity. Phytochemical analysis revealed the presence of coumarins, flavonoids, tannins, and triterpenes in the extract, which did not show toxicity to A. salina nauplii. No mortality and behavioral alterations were detected for mice treated with the extract (300 and 2000 mg/kg b.w.) for 14 days. However, animals that received the highest dose showed reduced MCV and MCHC as well as increased serum alkaline phosphatase activity. In treatments with the extract at both 300 and 2000 mg/kg, there was a reduction in number of leukocytes, with decrease in percentage of lymphocytes and increase in proportion of segmented cells. Histopathological analysis of organs from mice treated with the extract at 2000 mg/kg revealed turgidity of contorted tubules in kidneys, presence of leukocyte infiltration around the liver centrilobular vein, and high dispersion of the spleen white pulp. The extract showed antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Candida krusei, Candida tropicalis, and Aspergillus flavus. In conclusion, the extract contains antimicrobial agents and was not lethal for mice when ingested; however, its use requires caution because it promoted biochemical, hematological, and histopathological alterations. PMID:26246840

  7. Electrophysiological and behavioral responses of male fall webworm moths (Hyphantria cunea to Herbivory-induced mulberry (Morus alba leaf volatiles.

    Directory of Open Access Journals (Sweden)

    Rui Tang

    Full Text Available Volatile organic compounds (VOCs were collected from damaged and intact mulberry leaves (Morus alba L., Moraceae and from Hyphantria cunea larvae by headspace absorption with Super Q columns. We identified their constituents using gas chromatography-mass spectrometry, and evaluated the responses of male H. cunea antennae to the compounds using gas chromatography-flame ionization detection coupled with electroantennographic detection. Eleven VOC constituents were found to stimulate antennae of male H. cunea moths: β-ocimene, hexanal, cis-3-hexenal, limonene, trans-2-hexenal, cyclohexanone, cis-2-penten-1-ol, 6-methyl-5-hepten-2-one, 4-hydroxy-4-methyl-2-pentanone, trans-3-hexen-1-ol, and 2,4-dimethyl-3-pentanol. Nine of these chemicals were released by intact, mechanically-damaged, and herbivore-damaged leaves, while cis-2-penten-1-ol was released only by intact and mechanically-damaged leaves and β-ocimene was released only by herbivore-damaged leaves. Results from wind tunnel experiments conducted with volatile components indicated that male moths were significantly more attracted to herbivory-induced volatiles than the solvent control. Furthermore, male moths' attraction to a sex pheromone lure was increased by herbivory-induced compounds and β-ocimene, but reduced by cis-2-penten-1-ol. A proof long-range field trapping experiment showed that the efficiency of sex pheromone lures in trapping male moths was increased by β-ocimene and reduced by cis-2-penten-1-ol.

  8. Extraction, purification and anti-fatigue activity of γ-aminobutyric acid from mulberry (Morus alba L.) leaves

    Science.gov (United States)

    Chen, Hengwen; He, Xuanhui; Liu, Yan; Li, Jun; He, Qingyong; Zhang, Cuiying; Wei, Benjun; Zhang, Ye; Wang, Jie

    2016-01-01

    Mulberry (Morus alba L.) is a tree species of Moraceae widely distributed in Southern China. In the present study, the white crystal of γ-aminobutyric acid (GABA) was purified from mulberry leaves, and its bioactivity was also investigated. The main results were as follows: first, the crude GABA was extracted from mulberry leaves by using biochemical methods. Then, the crude was purified by chromatography over an S-8 macroporous resin, Sephadex G-10, and 732 cation exchange resin to yield a white crystal. Lavage administration and exposure of GABA to male NIH mice showed no adverse effects on their growth and development. In an endurance capacity test, the average loaded-swimming time of medium dose was 111.60% longer than the control (P < 0.01). Further investigations showed that relative to that of model control, the respective blood lactate (BL) concentrations of low- and medium-dose were 28.52% and 28.81% lower (P < 0.05), whereas the levels of blood urea nitrogen (BUN) were 36.83% and 40.54% lower (P < 0.05), and that of liver glycogen (LG) levels were 12.81% and 17.22% lower (P < 0.05). The results indicated that GABA has an advantage over taurine of anti-fatigue effect. These findings were indicative of the anti-fatigue activity of GABA.

  9. Schisandra chinensis and Morus alba Synergistically Inhibit In Vivo Thrombus Formation and Platelet Aggregation by Impairing the Glycoprotein VI Pathway

    Directory of Open Access Journals (Sweden)

    Dong-Seon Kim

    2017-01-01

    Full Text Available Morus alba L. (MAL extract has been used in traditional medicine for its cardioprotective and antiplatelet effects, while another herbal remedy, Schisandra chinensis (SCC, has been reported to have anti-inflammatory and antioxidant properties. We evaluated underlying cellular changes exerted by extracts of these plants on platelet function and effects of SCC + MAL on in vivo thrombus formation using AV shunt and tail thrombosis-length models in rats. In vitro platelet aggregation, granule secretion, and Ca2+i release assays were carried out. The activation of integrin αIIbβ3 and phosphorylation of downstream signaling molecules, including MAPK and Akt, were investigated using cytometry and immunoblotting, respectively. Scanning electron microscopy (SEM was used to evaluate changes in platelet shape and HPLC analysis was carried out to identify the marker compounds in SCC + MAL mixture. In vivo thrombus weight and average length of tail thrombosis were significantly decreased by SCC + MAL. In vitro platelet aggregation, granule secretion, Ca2+i release, and integrin αIIbβ3 activation were notably inhibited. SCC + MAL markedly reduced the phosphorylation of MAPK pathway factors along with Akt. HPLC analysis identified four marker compounds: isoquercitrin, astragalin, schizandrol A, and gomisin A. The extracts exerted remarkable synergistic effects as natural antithrombotic and antiplatelet agent and a potent drug candidate for treating cardiovascular diseases.

  10. Producción y calidad de la morera (morus alba cosechada en diferentes modalidades de poda

    Directory of Open Access Journals (Sweden)

    Carlos Boschini

    2001-01-01

    Full Text Available Producción y calidad de la biomasa de morera (Morus alba cosechada en diferentes modalidades de poda. Una plantación establecida en 1993, con un distanciamiento entre plantas de 60, 90 y 120 cm entre hileras y plantas, fue cosechada 26 veces a diferentes intervalos (56 a 112 días de poda durante cinco años consecutivos, a la altura de 60 cm sobre el nivel del suelo. Se practicaron tres tipos de poda: testigo, semilimpio y limpio. Con un intervalo de 84 días de crecimiento se cosecharon los rebrotes y se determin ó el contenido de materia seca, proteína cruda, fibra neutro detergente y cenizas totales. La poda semilimpia provocó una pérdida del 10% en la producción de materia seca de hoja por hectárea y un 13% en el rendimiento de tallo con respecto al tratamiento testigo. La cosecha conjunta de hojas y tallos decreció un 11%. La poda limpia provocó una disminuci ón de 32% en el rendimiento de materia seca de hoja por hectárea y 36% en la producción de tallo con relación al tratamiento testigo. La producción compuesta decreció 33%. La calidad nutricional de los rebrotes, hojas y tallos, fue similar entre los diferentes tratamientos de poda

  11. Electrophysiological and behavioral responses of male fall webworm moths (Hyphantria cunea) to Herbivory-induced mulberry (Morus alba) leaf volatiles.

    Science.gov (United States)

    Tang, Rui; Zhang, Jin Ping; Zhang, Zhong Ning

    2012-01-01

    Volatile organic compounds (VOCs) were collected from damaged and intact mulberry leaves (Morus alba L., Moraceae) and from Hyphantria cunea larvae by headspace absorption with Super Q columns. We identified their constituents using gas chromatography-mass spectrometry, and evaluated the responses of male H. cunea antennae to the compounds using gas chromatography-flame ionization detection coupled with electroantennographic detection. Eleven VOC constituents were found to stimulate antennae of male H. cunea moths: β-ocimene, hexanal, cis-3-hexenal, limonene, trans-2-hexenal, cyclohexanone, cis-2-penten-1-ol, 6-methyl-5-hepten-2-one, 4-hydroxy-4-methyl-2-pentanone, trans-3-hexen-1-ol, and 2,4-dimethyl-3-pentanol. Nine of these chemicals were released by intact, mechanically-damaged, and herbivore-damaged leaves, while cis-2-penten-1-ol was released only by intact and mechanically-damaged leaves and β-ocimene was released only by herbivore-damaged leaves. Results from wind tunnel experiments conducted with volatile components indicated that male moths were significantly more attracted to herbivory-induced volatiles than the solvent control. Furthermore, male moths' attraction to a sex pheromone lure was increased by herbivory-induced compounds and β-ocimene, but reduced by cis-2-penten-1-ol. A proof long-range field trapping experiment showed that the efficiency of sex pheromone lures in trapping male moths was increased by β-ocimene and reduced by cis-2-penten-1-ol.

  12. Descomposición del follaje de Leucaena leucocephala cv. Cunningham asociada con Morus alba var. tigríada

    Directory of Open Access Journals (Sweden)

    F Ruz Súarez

    Full Text Available El presente estudio se diseñó en una parcela experimental donde se utiliza, desde hace diez años, el follaje de Leucaena leucocephala (leucaena como abono verde en el cultivo de Morus alba (morera, con el objetivo de determinar la velocidad de descomposición del follaje de la leguminosa y la relación de este proceso con algunos de los factores bióticos y abióticos presentes en el sistema. Para el estudio de la dinámica de la descomposición del follaje se utilizó el método de bolsas de hojarasca (litter bags. El follaje depositado en estas bolsas fue evaluado en seis momentos durante la etapa de estudio, que abarcó dos años. En cada fecha de recolección, a la hojarasca remanente de cada bolsa se le determinó la población de macrofauna mediante la separación manual, según la metodología del Programa de Investigación Internacional "Biología y Fertilidad del Suelo Tropical" (TSBF. La composición taxonómica de la macrofauna asociada al proceso de descomposición del follaje de leucaena estuvo constituida por cuatro Phylum, seis clases y siete órdenes. El 97% de los organismos eran detritívoros y el 3% depredadores. El tipo de bolsa no influyó en la descomposición del follaje y los factores climáticos desempeñaron un rol decisivo en este proceso.

  13. Producción y valor nutricional del cultivo de morera (Morus alba L. con intercalamiento de leguminosas

    Directory of Open Access Journals (Sweden)

    María Judith Delgado

    2012-07-01

    Full Text Available Una de las principales preocupaciones en la alimentación animal es la búsqueda de alternativas rentables para el productor y eficientes desde el punto de vista nutricional para los animales. Para que el volumen de la producción de estos alimentos sea el adecuado, se han utilizado prácticas como las planteadas en la revolución verde, que incluyen el uso de fertilizantes nitrogenados químicos, lo que ha traído secuelas negativas sobre el medioambiente. En la búsqueda de nuevas alternativas nutricionales se encontraron algunos forrajes, como la morera (Morus alba L., que tiene un alto nivel de proteína, entre otras propiedades nutricionales; pero, alternativas nutricionales como ésta requieren fertilización nitrogenada en elevadas cantidades.Con el interés de generar alternativas amigables con el medioambiente y que además logren mantener o incrementar el volumen de producción y el valor nutricional de la morera, surgió la idea de estudiar la intersiembra de este arbusto con leguminosas, como arveja (Pisum sativum L., alfalfa (Medicago sativa L y fríjol (Phaseolus vulgaris L., analizando variables nutricionales y parámetros de crecimiento del cultivo de morera. Además, se trabajó con un grupo de fertilización nitrogenada y un grupo de control (sin modificación alguna del cultivo. Al finalizar el estudio se encontraron diferencias estadísticamente significativas (p<0.05 para la mayoría de parámetros, exceptuando la variable altura, que no presentó diferencias importantes (p>0.05.

  14. Comportamiento productivo de reproductoras porcinas alimentados con follaje fresco de Morus alba. IIndicadores hematológicos y estructurales

    Directory of Open Access Journals (Sweden)

    Contino Yuván; Ojeda Félix; Herrera Rafael; Altunaga Nancy; Pérez Guadalupe.

    2008-08-01

    Full Text Available El objetivo de esta investigación consistió en evaluar el comportamiento hematológico, morfométrico e histológico de las reproductoras porcinas alimentadas con follaje fresco de Morus alba (hojas y tallos tiernos como sustituto parcial del concentrado comercial en diferentes estados reproductivos comparadas con un grupo control el que no consumió el follaje fresco como sustituto parcial del concentrado. Las investigaciones se efectuaron en el Módulo de Investigación-Producción Porcina de la EEPF “Indio Hatuey. Los indicadores hematológicos medidos fueron el hematocrito, la hemoglobina y el leucograma con diferencial, en el Laboratorio Provincial del IMV ubicado en el municipio de Jovellanos. Elanálisis morfométrico de los órganos del tracto gastrointestinal,hemolinfopoyéticos y accesorios, se realizó mediante la medición y elpesaje en las reproductoras posterior al sacrificio. El rocesamiento histológico se realizó mediante la tinción hematoxilina-eosina. En el estudio estadístico se empleó el paquete SPSS Versión 10.1. Se obtuvo un mejor comportamiento hematológico, morfométrico e histológico al incluir en la dieta de las reproductoras el follaje de morera fresca como sustituto parcial del concentrado. Los resultados demuestran la factibilidad económica del empleo de esta alternativa alimentaría.

  15. Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.).

    Science.gov (United States)

    Lee, Ji Sun; Synytsya, Andriy; Kim, Hyun Bok; Choi, Doo Jin; Lee, Seul; Lee, Jisun; Kim, Woo Jung; Jang, Seongjae; Park, Yong Il

    2013-11-01

    A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole percent), arabinose (36.3%), and rhamnose (18.4%), while other major sugars such as glucose, xylose, mannose, and fucose were present as minor components. HPLC analysis revealed that JS-MP-1 contains both galacturonic acid (GalA) and glucuronic acid (GlcA) at approximately 4:1 in mole percent. Monosaccharide composition, Fourier-transform infrared (FTIR) analysis, biochemical analysis, and elemental analysis suggested that JS-MP-1 is an acidic heteropolysaccharide, most likely a rhamnoarabinogalacturonan type plant pectic polysaccharide, with an apparent molecular mass of 1600 kDa containing no, or if any, negligible level of sulfate esters and proteins. Enzyme-Linked Immunosorbent Assay and RT-PCR analysis demonstrated that JS-MP-1 significantly stimulates murine RAW264.7 macrophage cells to release chemokines (RANTES and MIP-1α) and proinflammatory cytokines (TNF-α and IL-6) and to induce the expression of iNOS and COX-2, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that the mulberry fruit-derived polysaccharide JS-MP-1 can act as a potent immunomodulator, and these observations may support the applicability of this polysaccharide as an immunotherapeutic adjuvant or the water extracts of the mulberry fruit as a beneficial health food.

  16. Atividade Anti-inflamatória e Avaliação da Toxicidade do Extrato Hidroetanólico de Morus alba (Moraceae

    Directory of Open Access Journals (Sweden)

    Camila Bugnotto Pereira

    2013-01-01

    Full Text Available No presente trabalho, avaliou-se a atividade antiinflamatória do extrato hidroetanólico das folhas de Morus alba, através do modelo de indução de tecido granulomatoso e analisaram-se os efeitos toxicológicos sobre o fígado pela dosagem de AST e ALT e sobre o rim, pela dosagem de creatinina. O extrato hidroetanólico das folhas de M. alba foi administrado oralmente, três vezes ao dia, durante 6 dias. A nimesulida (5 mg/kg/dia foi utilizada como controle positivo e o propilenoglicol 20% como controle negativo. Após o tratamento, foi avaliada a formação do granuloma e realizada a dosagem de AST, ALT e creatinina em todos os grupos. Os animais tratados com o extrato de M. alba apresentaram inibição do processo inflamatório de 20,24 ± 6,94%, enquanto que os tratados com controle positivo apresentaram 21,42 ± 6,52%. O extrato hidroetanólico de M. alba demonstrou atividade antiinflamatória semelhante à nimesulida com ausência de indícios de hepatotoxicidade e nefrotoxicidade.

  17. (Poly)phenolic fingerprint and chemometric analysis of white (Morus alba L.) and black (Morus nigra L.) mulberry leaves by using a non-targeted UHPLC-MS approach.

    Science.gov (United States)

    Sánchez-Salcedo, Eva M; Tassotti, Michele; Del Rio, Daniele; Hernández, Francisca; Martínez, Juan José; Mena, Pedro

    2016-12-01

    This study reports the (poly)phenolic fingerprinting and chemometric discrimination of leaves of eight mulberry clones from Morus alba and Morus nigra cultivated in Spain. UHPLC-MS(n) (Ultra High Performance Liquid Chromatography-Mass Spectrometry) high-throughput analysis allowed the tentative identification of a total of 31 compounds. The phenolic profile of mulberry leaf was characterized by the presence of a high number of flavonol derivatives, mainly glycosylated forms of quercetin and kaempferol. Caffeoylquinic acids, simple phenolic acids, and some organic acids were also detected. Seven compounds were identified for the first time in mulberry leaves. The chemometric analysis (cluster analysis and principal component analysis) of the chromatographic data allowed the characterization of the different mulberry clones and served to explain the great intraspecific variability in mulberry secondary metabolism. This screening of the complete phenolic profile of mulberry leaves can assist the increasing interest for purposes related to quality control, germplasm screening, and bioactivity evaluation. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Antidepressant-Like Effects of Sanggenon G, Isolated from the Root Bark of Morus alba, in Rats: Involvement of the Serotonergic System.

    Science.gov (United States)

    Lim, Dong Wook; Jung, Jae-Woo; Park, Ji-Hae; Baek, Nam-In; Kim, Yun Tai; Kim, In-Ho; Han, Daeseok

    2015-01-01

    The root bark of Morus alba is commonly used as an alternative medicine due to its numerous health benefits in humans. However, the antidepressant effects of various active components from M. alba have not been fully elucidated. In this study, we aimed to determine whether sanggenon G, an active compound isolated from the root bark of M. alba, exhibited antidepressant-like activity in rats subjected to forced swim test (FST)-induced depression. Acute treatment of rats with sanggenon G (30 mg/kg, intraperitoneally (i.p.)) significantly reduced immobility time and increased swimming time without any significant change in climbing. Rats treated with sanggenon G also exhibited a decrease in the limbic hypothalamic-pituitary-adrenal (HPA) axis response to the FST, as indicated by attenuation of the corticosterone response and decreased c-Fos immunoreactivity in the hypothalamic paraventricular nucleus (PVN). In addition, the antidepressant-like effects of sanggenon G were significantly inhibited by WAY100635 (1 mg/kg, i.p.; a selective 5-hydroxytryptamine1A (5-HT1A) receptor antagonist), but not SCH23390 (0.05 mg/kg, i.p.; a dopamine D1 receptor antagonist). Our findings suggested that the antidepressant-like effects of sanggenon G were mediated by an interaction with the serotonergic system. Further studies are needed to evaluate the potential of sanggenon G as an alternative therapeutic approach for the treatment of depression.

  19. RP-HPLC测定桑椹中芦丁的含量%Determination of Rutin in Morus alba by RP-HPLC

    Institute of Scientific and Technical Information of China (English)

    耿旦; 马雯芳; 甄汉深; 丘琴; 韦良宏; 唐霞丽

    2011-01-01

    目的:优化桑椹中芦丁的提取条件并建立芦丁的HPLC测定方法.方法:采用单因素考察法优化桑椹中芦丁的提取条件,采用HPLC法测定芦丁的含量,色谱柱为Agilent Zobax C18(4.6 mm×250 mm,5 μm),以乙腈-0.1%磷酸(19:81)为流动相,流速1.0 mL·min-1,检测波长360 nm,柱温30℃.结果:芦丁最佳提取条件为料液比1:25,50%乙醇回流60min,芦丁在0.1~2.0μg有良好线性关系(r=0.999 9).平均回收率为102.4%,RSD 1.8%(n=6).结论:该方法简便、准确、可靠,可作为桑椹中芦丁含量测定的方法.%Objective:Optimize the extract condition of rutin in Morus alba to establish a quantification method for rutin. Method: Single-factor analysis was applied to optimize the extract condition of rutin in Morus alba HPLC was used in the determination of rutin on C18 column (4.6 mm × 250 mm, 5 μm) with a mixture of acetonitrile-0. 1% phosphoric acid (19:81 ) as a mobile phase at the flow rate of 1.0 mL·min-1. Rutin was detected at 360 nm, column temperature was 30 ℃. Result: The optimal extract condition was the solid-to-liquid ratio 1: 25, 50% ethanol refluxed for 60 min. The linearity of rutin was in the range of 0. 1-2.0 μg. The average recovery rate of rutin was 102.4% with RSD 1.8% (n =6). Conclusion: The determination method was simple,accurate and reliable, it could be used as the determination method of rutin in Morus alba.

  20. Nonclinical Safety Assessment of Morus alba L. Fruits: Study of 90-D Toxicity in Sprague Dawley Rats and Genotoxicity in Salmonella.

    Science.gov (United States)

    Chang, Bo Yoon; Kim, Seon Beom; Lee, Mi Kyeong; Park, Hyun; Kim, Sung Yeon

    2016-05-01

    Morus alba L. is a traditional herb with a long history of consumption, both as an edible fruit and as medicine. However, its safety evaluation has not yet been established. The objective of this study was to evaluate subchronic oral toxicity and genotoxicity of M. alba L. fruits (MFE). The subchronic toxicity after daily oral administration of MFE at 0, 40, 200, and 1000 mg/kg for 90 d was examined in Sprague Dawley (SD) rats. MFE administration did not lead to death, adverse effects, change in food and water consumption, and body weight gain. Significant toxic effects were not found within the parameters of organ weight, biochemical values, and hematological and urine analysis between the control and the MFE group. The genotoxicity of MFE was assayed by Ames test in Salmonella typhimurium strains TA98, TA102, and TA1535. No genotoxicity was found in all the tested strains. Thus in this study, a no-observed-adverse-effect level for MFE in 90 d repeated oral toxicity study in rats was determined to be greater than 1000 mg/kg regardless of gender. The results also suggested that MFE does not have a genotoxicity potential.

  1. Neuroprotective effect of prenylated arylbenzofuran and flavonoids from morus alba fruits on glutamate-induced oxidative injury in HT22 hippocampal cells.

    Science.gov (United States)

    Seo, Kyeong-Hwa; Lee, Dae-Young; Jeong, Rak-Hun; Lee, Dong-Sung; Kim, Young-Eon; Hong, Eock-Kee; Kim, Youn-Chul; Baek, Nam-In

    2015-04-01

    A prenylated arylbenzofuran and six flavonoids were isolated from the fruits of Morus alba L. through silica gel, octadecyl silica gel, and Diaion HP-20 column chromatography. Based on the nuclear magnetic resonance, mass spectrometry, and infrared spectroscopic data, the chemical structures of the compounds were determined to be artoindonesianin O (1), isobavachalcone (2), morachalcone A (3), quercetin (4), astragalin (5), isoquercetin (6), and rutin (7). The isolated compounds were evaluated for protection of HT22-immortalized hippocampal cells against glutamate-induced oxidative stress. Compounds 1 and 3 exhibited protective effects with EC(50) values of 19.7±1.2 and 35.5±2.1 μM, respectively. The major compounds 1-3 and 7 were quantified using liquid chromatography/mass spectrometry analysis and were determined to be 1.88±2.1, 1.90±1.8, 0.78±1.5, and 37.29±2.2 mg/kg, respectively, in the ethanol extract of M. alba L. fruits.

  2. Integrated HPTLC-based Methodology for the Tracing of Bioactive Compounds in Herbal Extracts Employing Multivariate Chemometrics. A Case Study on Morus alba.

    Science.gov (United States)

    Chaita, Eliza; Gikas, Evagelos; Aligiannis, Nektarios

    2017-03-01

    In drug discovery, bioassay-guided isolation is a well-established procedure, and still the basic approach for the discovery of natural products with desired biological properties. However, in these procedures, the most laborious and time-consuming step is the isolation of the bioactive constituents. A prior identification of the compounds that contribute to the demonstrated activity of the fractions would enable the selection of proper chromatographic techniques and lead to targeted isolation. The development of an integrated HPTLC-based methodology for the rapid tracing of the bioactive compounds during bioassay-guided processes, using multivariate statistics. Materials and Methods - The methanol extract of Morus alba was fractionated employing CPC. Subsequently, fractions were assayed for tyrosinase inhibition and analyzed with HPTLC. PLS-R algorithm was performed in order to correlate the analytical data with the biological response of the fractions and identify the compounds with the highest contribution. Two methodologies were developed for the generation of the dataset; one based on manual peak picking and the second based on chromatogram binning. Results and Discussion - Both methodologies afforded comparable results and were able to trace the bioactive constituents (e.g. oxyresveratrol, trans-dihydromorin, 2,4,3'-trihydroxydihydrostilbene). The suggested compounds were compared in terms of Rf values and UV spectra with compounds isolated from M. alba using typical bioassay-guided process. Chemometric tools supported the development of a novel HPTLC-based methodology for the tracing of tyrosinase inhibitors in M. alba extract. All steps of the experimental procedure implemented techniques that afford essential key elements for application in high-throughput screening procedures for drug discovery purposes. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  3. Morus alba o Hibiscus rosa-sinensis como sustituto parcial de soya en dietas integrales para conejos

    National Research Council Canada - National Science Library

    Lara, P.E; Itza, M.F; Sangines, J.R; Magana, M.A

    2012-01-01

    ... cecotrofia. Los objetivos del presente trabajo fueron: evaluar la tasa de crecimiento y el rendimiento de la canal en conejos alimentados con minibloques de harina de morera (Moras alba) o tulipan...

  4. 桑叶正己烷提取物的活性组分分析%Analysis of Active Components of Hexane Extractives of Morus alba Leaves

    Institute of Scientific and Technical Information of China (English)

    刘志明; 王海英; 张爽

    2011-01-01

    [目的]分析桑叶正己烷提取物的活性组分,探究桑叶中的活性化合物.[方法]桑叶正己烷提取物进行抑菌试验并通过气相色谱-质谱分析抑菌活性最强的桑叶正己烷提取物的挥发性组分.[结果]抑菌试验结果表明6、7、8月桑叶正己烷提取物对金黄色葡萄球菌均有抑制作用,对大肠杆菌均无抑制作用;且6月桑叶正己烷提取物对金黄色葡萄球菌抑制作用最强(抑菌圈直径为10.95 mm).6月桑叶正己烷提取物气相色谱-质谱分析结果表明其主要挥发性组分为十四烷(16.76%),十二烷(13.20%),邻苯二甲酸二异丁酯(10.26%),癸烷(9.10%),十六烷(8.71%),亚麻醇(7.25%),十八烷(5.88%),二十烷(3.26%),邻苯二甲酸二丁酯(2.59%).[结论]亚麻醇是具有抑菌潜力的化合物.%[Objective]The aim was to explore the active compound of Morus alba leaves with the analysis of active components of hexane extractives of M.alba leaves.[Method]Antibacterial assays of hexane extractives of M.alba leaves were done and the volatile components of hexane extractives of M.alba leaves with the strongest antibacterial activity were analyzed by gas chromatography-mass spectromretry(GC-MS).[Result]The results of inhibitory effect of hexane extractives of M.alba leaves on Staphylococcus aureus and Escherichia coli showed that hexane extractives of M.alba leaves collected in June, July and August had antibacterial activity against S.aureus and had no antibacterial effect against E.coli Furthermore, hexane extractives of M.alba leaves collected in June had the strongest antibacterial activity against S.aureus (inhibition diameter 10.95 mm).The hexane extractives of M.alba leaves collected in June was analyzed by GC-MS, the main volatile components were tetradecane ( 16.76% ), dodecane ( 13.20% ), diisobutyl phthalate ( 10.26% ), decane ( 9.10% ), hexadecane ( 8.71% ), linolenyl alcohol (7.25%), octadecane (5.88%), eicosane (3.26%), dibutyl

  5. Electrophysiological Responses and Reproductive Behavior of Fall Webworm Moths (Hyphantria cunea Drury are Influenced by Volatile Compounds from Its Mulberry Host (Morus alba L.

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    Rui Tang

    2016-05-01

    Full Text Available Hyphantria cunea (Drury is an invasive pest of Morus alba L. in China. β-ocimene and cis-2-penten-1-ol among eleven electro-physiologically active leaf volatiles from M. alba have been reported to influence captures of Hyphantria cunea moths when added into sex pheromone traps. This study further investigated influences of volatile types and their dosages on the electro-physiological responses in the antennae of male and female moths, as well as on mating and oviposition behaviors. Females were, regardless of dosages, more sensitive to β-ocimene and cis-2-penten-1-ol in electro-physiological response tests than males. For males, a dose response was detected, i.e., a dosage of 10 μg and 100 μg of either chemical stimulated higher electric response in their antennae than 1 μg. Moth pairs either exposed respectively to a herbivore-induced M. alba volatile blend (HIPV, to a mechanically-damaged M. alba volatile blend (MDV, to β-ocimene, to cis-2-penten-1-ol, or to pentane as a control showed that pairs exposed to β-ocimene most likely mated, followed by HIPV blends and least by the other volatiles or the control. In contrast, β-ocimene induced about 70% of the female oviposition behaviors and was nearly 4.5 times the oviposition rate than cis-2-penten-1-ol and 2 times than the control. However, none of the chemicals had any effect on the 48 h fecundity or on egg sizes. In conclusion, β-ocimene from mulberry plants alone could promote mating and oviposition in H. cunea at a dosage of 1 mg. The results indicate that reproductive behaviors of H. cunea moths can be enhanced through HIPV blends and β-ocimene induced by feeding of larvae. This contra phenomenon has revealed a different ecology in this moth during colonizing China as local pests would commonly be repelled by herbivore induced chemicals. These chemicals can be used for the development of biological control approaches such as being used together with sex pheromone traps.

  6. The anti-angiogenic herbal composition Ob-X from Morus alba, Melissa officinalis, and Artemisia capillaris regulates obesity in genetically obese ob/ob mice.

    Science.gov (United States)

    Yoon, Michung; Kim, Min-Young

    2011-06-01

    The growth and development of adipose tissue leading to obesity is suggested to depend on angiogenesis. Our previous study showed that Melissa officinalis L. (Labiatae), Morus alba L. (Moraceae), and Artemisia capillaris Thunb. (Compositae) are involved in the regulation of angiogenesis. We hypothesized that Ob-X, a mixture of three herbs, M. alba, M. officinalis, and A. capillaris, can regulate obesity. To investigate the inhibitory effect of Ob-X on obesity in genetically obese ob/ob mice. The effect of Ob-X on angiogenesis was measured using a mouse Matrigel plug assay. The effects of Ob-X on obesity were investigated in ob/ob mice. Ob-X inhibited angiogenesis in a dose-dependent manner, as evidenced by decreased blood vessel density in a mouse matrigel plug assay. Administration of Ob-X to ob/ob mice for 5 weeks produced a significant reduction in body weight gain by 27% compared with control (12.1 ± 3.01 vs. 16.6 ± 2.24 g, respectively). Ob-X also significantly decreased visceral adipose tissue mass by 15% (0.87 ± 0.12 vs. 1.02 ± 0.15 g, respectively). The size of adipocytes in visceral adipose tissue was reduced by 46% in Ob-X-treated mice. Ob-X treatment inhibited hepatic lipid accumulation and significantly decreased circulating glucose levels compared with controls (197 ± 56.5 vs. 365 ± 115 mg/dL, respectively). These results suggest that Ob-X, which has an anti-angiogenic activity, reduces body weight gain and visceral adipose tissue mass in genetically obese mice, providing evidence that obesity can be prevented by angiogenesis inhibitors.

  7. Selection and characterisation of lactic acid bacteria isolated from different origins for ensilingRobinia pseudoacacia and Morus alba L. leaves

    Institute of Scientific and Technical Information of China (English)

    NI Kui-kui; YANG Hui-xiao; HUA Wei; WANG Yan-ping; PANG Hui-li

    2016-01-01

    The objective of this study was to isolate lactic acid bacteria (LAB) strains from different origins and to select the best strains for ensilingRobinia pseudoacacia (RB) and Morus albaL. (MB)leaves. The LAB strains were inoculated into the extracted liquid obtained from RB and MB leaves to evaluate the fermentation products. 11 LAB strains were selected for further experiments based on the highest products of lactic or acetic acid, including 1 strain of Weissela confusa, 2 ofLactobacilus reuteri and 8 ofLactobacilus plantarum.The API 50 CH fermentation experiment indicated that al of the selected 11 LAB strains utilised most of the carbohydrates. Al the strains grew at temperatures between 10 and 45°C and at a pH of 3.5 to 4.5; however,L. reuteri F7 and F8 tolerated a pH as low as 3.0. Al 11 LAB strains showed antibacterial activity against Listeria monocytogens,Escherichia coil,Salmonelasp. and Acetobacter pasteurianus; however, after excluding the effect of organic acids, only F7 and F8 stil exhibited antibacterial activity. The present study indicated that the selected 11 LAB strains could be used to prepare silages of RB and MB leaves, especialyL. reuteri F7 and F8.

  8. Inhibitory effects of constituents from Morus alba var. multicaulis on differentiation of 3T3-L1 cells and nitric oxide production in RAW264.7 cells.

    Science.gov (United States)

    Yang, Zhi-Gang; Matsuzaki, Keiichi; Takamatsu, Satoshi; Kitanaka, Susumu

    2011-07-19

    A new arylbenzofuran, 3',5'-dihydroxy-6-methoxy-7-prenyl-2-arylbenzofuran (1), and 25 known compounds, including moracin R (2), moracin C (3), moracin O (4), moracin P (5), artoindonesianin O (6), moracin D (7), alabafuran A (8), mulberrofuran L (9), mulberrofuran Y (10), kuwanon A (11), kuwanon C (12), kuwanon T (13), morusin (14), kuwanon E (15), sanggenon F (16), betulinic acid (17), uvaol (18), ursolic acid (19), β-sitosterol (20), oxyresveratrol 2-O-β-D-glucopyranoside (21), mulberroside A (22), mulberroside B (23), 5,7-dihydroxycoumarin 7-O-β-D-glucopyranoside (24), 5,7-dihydroxycoumarin 7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (25) and adenosine (26), were isolated from Morus alba var. multicaulis Perro. (Moraceae). Their structures were determined by spectroscopic methods. The prenyl-flavonoids 11-14, 16, triterpenoids 17,18 and 20 showed significant inhibitory activity towards the differentiation of 3T3-L1 adipocytes. The arylbenzofurans 1-10 and prenyl-flavonoids 11-16 also showed significant nitric oxide (NO) production inhibitory effects in RAW264.7 cells.

  9. Determination of 1-deoxynojirimycin in Morus alba L. leaves by derivatization with 9-fluorenylmethyl chloroformate followed by reversed-phase high-performance liquid chromatography.

    Science.gov (United States)

    Kim, Jin-Won; Kim, Soo-Un; Lee, Heui Sam; Kim, Iksoo; Ahn, Mi Young; Ryu, Kang Sun

    2003-06-20

    A rapid and reliable method suitable for assays of a large number of Morus alba leaves for 1-deoxynojirimycin (DNJ) has been developed. DNJ in 0.1 g of freeze-dried leaves was double-extracted in 10 mL of aqueous 0.05 M HCl by vortexing for 15 s at room temperature, derivatized with 9-fluorenylmethyl chloroformate (FMOC-Cl), and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) equipped with a fluorescence detector. The double extraction recovered > 99% of extractable DNJ from the leaves. Stabilization of FMOC-derivatized DNJ (DNJ-FMOC) was achieved by diluting the reactant with aqueous acetic acid after derivatization. DNJ-FMOC was stable for at least 16 days under acidic conditions at room temperature (24 degrees C). Linearity ranged between 0.3 and 30 microg mL(-1). The intra- and inter-day precision for DNJ-spiked biological samples was between 0.6 and 1.8% and between 3.7 and 4.5%, respectively.

  10. Produção e qualidade de folhas de amoreira em função da época do ano e de colheita

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    Miranda José Ednilson

    2002-01-01

    Full Text Available A quantidade e qualidade das folhas de amoreira (Morus alba L. exercem influência marcante sobre o desenvolvimento do bicho-da-seda e são, por sua vez, influenciadas por fatores como a idade das folhas, época do ano e características genéticas dos cultivares. Avaliou-se a produção e a qualidade nutricional de folhas de cinco cultivares de amoreira em diferentes épocas do ano e de colheita, objetivando verificar sua adaptação na região de Jaboticabal (SP. As folhas dos cultivares 'Miura', 'Korin', 'Calabresa', 'FM86' e 'FM Shima-Miura' foram colhidas na primavera, verão e outono após 45, 60, 75 e 90 dias da poda (DAP e avaliadas quanto à produção de folhas níveis de proteína bruta (PB e fibra em detergente neutro (FDN. As melhores produções foram verificadas em 'FM Shima-Miura', principalmente aos 75 DAP na primavera e no verão e aos 90 DAP no outono. No verão, as colheitas efetuadas aos 60, 75 e 90 DAP resultaram em produções mais elevadas. As épocas de colheita que resultaram em maiores níveis de PB foram aos 45 e 60 DAP e em menores níveis de FDN aos 60 DAP, respectivamente. Foi possível conciliar altos níveis de PB, baixos níveis de FDN e relativamente alta produção de massa foliar somente na colheita efetuada aos 75 DAP.

  11. Antidiabetic effects of Morus alba fruit polysaccharides on high-fat diet- and streptozotocin-induced type 2 diabetes in rats.

    Science.gov (United States)

    Jiao, Yukun; Wang, Xueqian; Jiang, Xiang; Kong, Fansheng; Wang, Shumei; Yan, Chunyan

    2017-03-06

    Type 2 diabetes mellitus (T2DM) is becoming a serious threat to human health. The fruit of Morus alba L. is widely used as a traditional Chinese medicine for the treatment of DM, dizziness, tinnitus, insomnia, and premature graying, as well as to protect the liver and kidneys. Several studies have demonstrated that the aqueous extracts of the roots bark, leaves, and ramuli of mulberry, which are known to contain polyphenols and polysaccharides, have antihyperglycemic and antihyperlipidemic activities. The aim of the present study was to further investigate the active polysaccharides from M. alba fruit by evaluating the antidiabetic activities of different fractions on T2DM rats and elucidate the mechanism underlying these activities. Diabetic rats were treated with two fractions of M. alba fruit polysaccharides (MFP50 and MFP90). The disease models were induced by a high-fat diet and low dose injection of streptozotocin and were compared to normal rats and metformin-treated diabetic rats. After seven weeks, the fasting blood glucose (FBG), oral glucose tolerance test (OGTT), fasting serum insulin (FINS) levels, homeostasis model of assessment-insulin resistance (HOMA-IR), glycated serum protein (GSP), and serum alanine transaminase (ALT) levels, as well as serum lipid profiles and histopathological changes in the pancreas were measured. Next, the expressions of the insulin signaling pathway were measured by western blot analysis to elucidate the potential mechanism underlying these antidiabetic activities. After seven weeks of treatment, a significant reduction in the FBG levels, OGTT-area under the curve (OGTT-AUC), FINS, HOMA-IR, ALT, and triglyceride (TG) values of the MFP50 group was observed. On the other hand, in the MFP90 group, the FBG, OGTT-AUC, FINS, HOMA-IR, GSP, and TG levels were significantly reduced. The level of high-density lipoprotein cholesterol (HDL-c) and the proportion of HDL-c to total cholesterol (TC) significantly increased in the MFP50

  12. Observation and Comparison of Pollen Submicroscopic Morphology of Seven Mulberry (Morus alba Linn.) Cultivars%Observation and Comparison of Pollen Submicroscopic Morphology of Seven Mulberry (Morus alba Linn.) Cultivars

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] This study aimed to investigate the submicroscopic structure and morphological characteristics of mulberry pollen. [Method] Mulberry pollen of seven cultivars were treated by glutaraldehyde fixing, alcohol gradient dehydration, freeze-drying and metal plating to observe the size and surface ornamentation under the scanning electron microscope (SEM). [Result] The pollen surface characteristics and ornamentation were clearly visible, indicating that this method is suitable for SEM observation of mulberry pollen; experimental mulberry pollen was nearly spher- ical, with two germination apertures and processes on aperture membrane; polar axis was 15.99-22.63 μm long, and equatorial axis was 14.98-20.78 μm long; the pollen volume of "Yu No.2' was the maximum, while that of "Jinxuan No.7' was the minimum; the surface of mulberry pollen showed smooth and tumor-like protuberance ornamentations, covered with different densities of particles. [Conclusion] Glutaralde- hyde fixative-alcohol gradient dehydration is an ideal method for morphological char- acteristics of mulberry pollen. This study may provide useful information for palynology identification of Morus plants or even species-level systematic classification.

  13. The anti-angiogenic herbal extracts Ob-X from Morus alba, Melissa officinalis, and Artemisia capillaris suppresses adipogenesis in 3T3-L1 adipocytes.

    Science.gov (United States)

    Hong, Yeonhee; Kim, Min-Young; Yoon, Michung

    2011-08-01

    Growing adipose tissue is thought to require adipogenesis, angiogenesis, and extracellular matrix (ECM) remodeling. Close examination of developing adipose tissue microvasculature reveals that angiogenesis often precedes adipogenesis. Since our previous study demonstrated that Ob-X, the anti-angiogenic herbal composition composed of Melissa officinalis L. (Labiatae), Morus alba L. (Moraceae), and Artemisia capillaris Thunb. (Compositae), reduced adipose tissue mass in obese mice, we hypothesized that adipogenesis can be inhibited by Ob-X. To investigate the effects of the anti-angiogenic herbal extracts Ob-X on adipogenesis in 3T3-L1 adipocytes. After differentiated 3T3-L1 adipocytes were treated with Ob-X, we studied the effects of Ob-X on triglyceride accumulation and expression of genes involved in adipogenesis, angiogenesis, and ECM remodeling. Treatment of cells with Ob-X inhibited lipid accumulation and adipocyte-specific gene expression caused by troglitazone or monocyte differentiation-inducing (MDI) mix. Ob-X reduced mRNA levels of angiogenic factors (vascular endothelial growth factor-A, -B, -C, -D, and fibroblast growth factor-2) and matrix metalloproteinases (MMPs; MMP-2 and MMP-9), whereas it increased mRNA levels of angiogenic inhibitors [(thrombospondin-1, tissue inhibitor of metalloproteinase-1 (TIMP-1), and TIMP-2)] in differentiated cells. MMP-2 and MMP-9 activities were also decreased in Ob-X-treated cells. These results suggest that the anti-angiogenic herbal composition Ob-X inhibits differentiation of preadipocytes into adipocytes. These events may be mediated by changes in the expression of genes involved in lipogenesis, angiogenesis, and the MMP system. Thus, by reducing adipogenesis, anti-angiogenic Ob-X provides a possible therapeutic approach for the prevention and treatment of human obesity and its related disorders.

  14. Composición química de la morera (morus alba, para uso en la alimentación animal: densidades y frecuencias de poda

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    Carlos Boschini F.

    2000-01-01

    Full Text Available Composición química de la morera (Morus alba, para uso en la alimentación animal: densidades y frecuencias de poda. El presente trabajo se llevó a cabo en la Estación Experimental de Ganado Lechero “Alfredo Volio Mata”, Cartago, Costa Rica. Las muestras fueron obtenidas de una plantación de morera dividida en tres lotes, con un distanciamiento entre plantas de 60, 90 y 120 cm entre hileras y plantas. Cada parcela se uniformó en dos alturas de poda, 30 y 60 cm sobre el nivel del suelo. Las subparcelas se podaron en forma consecutiva cada 56, 84 y 112 días durante 336 días. Los factores distancia se siembra, altura de poda y frecuencia de corte, influyeron sobre los contenidos químicos de la materia seca. La distancia de siembra y la altura de poda produjeron pequeñas variaciones en la composición de la hojas y en los tallos de la morera. La frecuencia de corte produjo cambios en la composición del las hojas y tallos de la morera; sin embargo, las diferencias en materia seca, proteína, carbohidratos estructurales o en las cenizas totales de las hojas no fueron superiores al 3% entre las frecuencias experimentadas. En el tallo los cambios mayores fueron de un 9% y se produjeron en los contenidos de materia seca, pared celular y en algunos de sus constituyentes estructurales como la celulosa. El número de corte dentro de cada frecuencia influyó de manera significativa sobre las proporciones químicas de hojas y tallos

  15. In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract

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    Ghimeray AK

    2015-07-01

    Full Text Available Amal Kumar Ghimeray,1 Un Sun Jung,1,2 Ha Youn Lee,1 Young Hoon Kim,1 Eun Kyung Ryu,1 Moon Sik Chang11R&D Center, Natural Solution Co., Ltd, Gojan-dong, Namdong-gu, Incheon, Republic of Korea; 2Department of Horticultural Biotechnology, Kyung Hee University, Yongin, Republic of KoreaBackground: In phytotherapy, the therapeutic potential is based on the combined action of different herbal drugs. Our objective was to evaluate the antioxidant, anti-collagenase (in vitro, and anti-wrinkle (in vivo effect of combined formulation containing Ginkgo biloba, Punica granatum, Ficus carica, and Morus alba fruits extract.Methods: Antioxidant evaluation was based on the scavenging activity of free radicals (1,1-diphenyl-2-picrylhydrazyl, H2O2, and O2- and the anti-collagenase activity was based on the reduction of collagenase enzyme in vitro. In an in vivo study, 21 female subjects were examined in a placebo-controlled trail. Facial wrinkle, especially the crow's feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo.Results: In the in vitro study, the combination of fruits extract showed a higher antioxidant activity which was comparable with the positive standard (ascorbic acid, butylated hydroxyanisole, and Trolox. The data also showed a dose-dependent inhibition of collagenase. In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively.Conclusion: The combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin.Keywords: antioxidant, anti-collagenase, anti-wrinkle, fruits, topical formulation

  16. Efecto de la inclusión de diferentes niveles de morera (Morus alba en la calidad nutricional de ensilajes de sorgo (Sorghum almum

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    A Alpízar

    Full Text Available El objetivo de esta investigación fue determinar el efecto de la inclusión de diferentes niveles de morera (Morus alba en la calidad nutricional de ensilajes de sorgo (Sorghum almum. Se evaluaron cinco proporciones de sorgo:morera (100:0, 75:25, 50:50, 25:75 y 0:100, con un diseño completamente aleatorizado y tres réplicas por cada tratamiento. Las mezclas se hicieron en base fresca y se colocaron en tanques plásticos de 120 L. La compactación se realizó en capas de 20 cm, mediante el apisonamiento con los pies, y se adicionó melaza a razón del 4 % del peso del material verde. Los ensilajes fueron almacenados durante 40 días. Antes y después de la conservación, se determinó: la materia seca (MS, la proteína cruda (PC, la fibra neutro detergente (FND, la fibra ácido detergente (FAD, la hemicelulosa y las cenizas. Con el aumento del porcentaje de morera se incrementaron de manera significativa los contenidos de MS, PC y cenizas (p < 0,05. La FND, la FAD y la hemicelulosa tendieron a aumentar después de la conservación. Los valores de pH de las mezclas 100:0 (3,81, 75:25 (3,91 y 50:50 (3,89 tuvieron diferencias significativas (p < 0,01 respecto a 75:25 (4,05 y 0:100 (4,08. La valoración organoléptica mostró un proceso de conservación adecuado en todas las mezclas. Se concluye que la inclusión de 50 % de morera constituye una alternativa viable para mejorar la calidad nutricional del ensilaje de sorgo.

  17. UP1304, a botanical composition containing two standardized extracts of Curcuma longa and Morus alba, mitigates pain and inflammation in Adjuvant-induced arthritic rats

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    Mesfin Yimam

    2016-01-01

    Full Text Available Background: Though, the initial etiologies of arthritis are multifactorial, clinically, patients share pain as the prime complaints. Present day pain relief therapeutics heavily relies on the use of prescription and over the counter nonsteroidal anti-inflammatory drugs as the first line of defense where their long-term usage causes gastrointestinal and cardiovascular-related side effects. Hence, the need for evidence-based safer and efficacious alternatives from natural sources to overcome the most prominent and disabling symptoms of arthritis is an overdue. Here, we evaluated the anti-inflammatory and analgesic effect of UP1304, a composition that contains a standardized blend of two extracts from the rhizome of Curcuma longa and the root bark of Morus alba in adjuvant-induced arthritis models in rats. Materials and Methods: The anti-inflammatory and analgesic effects of the botanical composition were demonstrated in adjuvant-induced arthritis models in rats with oral dose ranges of 50–200 mg/kg. Ibuprofen at a dose of 100 mg/kg was used as a reference compound. Ex vivo sulfated glycosaminoglycan inhibition assays were performed. Results: Statistically significant improvements in pain resistance, suppression of paw edema and ankle thickness were observed in animals treated with UP1304 compared to vehicle-treated diseased rats. These results were similar to those achieved by ibuprofen treatment. Inhibitions of proteoglycan degradation were observed in a range of 37.5–61.7% for concentration of UP1304 at 50–200 μg/mL when compared to interleukin-1α-exposed untreated explants. Conclusions: These data suggest that UP1304, for its analgesic and anti-inflammatory effects, could potentially be considered agent of botanical origin for the improvement of arthritis associated symptoms.

  18. Analgesic and anti-Inflammatory effect of UP3005, a botanical composition Containing two standardized extracts of Uncaria gambir and Morus alba

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    Mesfin Yimam

    2015-01-01

    Full Text Available Background: Osteoarthritis (OA is a chronic debilitating degenerative joint disease characterized by cartilage degradation and synovial inflammation exhibited by clinical symptoms such as joint swelling, synovitis, and inflammatory pain. Present day pain relief therapeutics heavily relies on the use of prescription and over the counter nonsteroidal anti-inflammatory drugs as the first line of defense where their long-term usage causes detrimental gastrointestinal and cardiovascular-related side-effects. As a result, the need for evidence based safer and efficacious alternatives from natural sources to overcome the most prominent and disabling symptoms of arthritis is a necessity. Materials and Methods: Describe the anti-inflammatory and analgesic effect of UP3005, a composition that contains a standardized blend of two extracts from the leaf of Uncaria gambir and the root bark of Morus alba in carrageenan-induced rat paw edema, abdominal constriction (writhing′s and ear swelling assays in mouse with oral dose ranges of 100-400 mg/kg. Results: In vivo, statistically significant improvement in pain resistance, and suppression of paw edema and ear thickness in animals treated with UP3005 were observed compared with vehicle-treated diseased rats and mice. Ibuprofen was used a reference compound in all the studies. In vitro, enzymatic inhibition activities of UP3005 were determined with IC50 values of 12.4 μg/ml, 39.8 μg/ml and 13.6 μg/ml in cyclooxygenase-2 (COX-1, COX-2 and lipoxygenase (5-LOX enzyme activity assay, respectively. Conclusions: These data suggest that UP3005, analgesic and anti-inflammatory agent of botanical origin with balanced dual COX-LOX inhibition activity, could potentially be used for symptom management of OA.

  19. Efecto de la fertilización biológica y/o mineral en la producción de forraje de morera (Morus alba L. (Nota técnica Effect of biological and/or mineral fertilization on mulberry (Morus alba L. forage production (Technical note

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    Katerine Oropesa

    2011-09-01

    Full Text Available Se realizó una investigación durante un año en áreas de la EEPF “Indio Hatuey”, con el objetivo de estudiar la respuesta morfoagronómica de Morus alba L. cv. Tigreada asociada con Canavalia ensiformis como abono verde, inoculada con micorrizas a través del producto EcoMic® (fertilización biológica, y su combinación o no con fertilización mineral. Los tratamientos consistieron en: testigo (T, fertilización mineral (FM, fertilización biológica (FB y fertilización combinada mineral y biológica (FM+ FB en ambas épocas del año. La fertilización mineral consistió en N y K, a razón de 300 y 150 kg/ha/año, respectivamente. El intercalamiento de la canavalia como abono verde inoculada con HMA, condicionó en el período lluvioso una mejora en la respuesta productiva, sin afectar el aprovechamiento del forraje como biomasa comestible (6 250 kg de hojas/ha y 18 194,4 kg de biomasa combustible. Se recomienda continuar los estudios para determinar la combinación óptima de fertilización mineral y biológica que permita incrementar el rendimiento y la relación tallo tierno:tallo leñoso.A study was conducted during a year in areas of the EEPF “Indio Hatuey”, in order to study the morphoagronomic response of Morus alba L. cv. Tigreada associated to Canavalia ensiformis as green manure, inoculated with mycorrhizas through the product EcoMic® (biological fertilization, and their combination or not with mineral fertilization. Treatments consisted in: control (C, mineral fertilization (MF, biological fertilization (BF and combined mineral and biological fertilization (MF + BF in both seasons. Mineral fertilization consisted in N and K, at a rate of 300 and 150 kg/ha/year, respectively. The intercropping of C. ensiformis as green manure inoculated with AMF, caused in the rainy season an improvement of the productive response, without affecting the utilization of forage as edible biomass (6 250 kg of leaves/ha and 18 194,4 kg of

  20. Efecto del tipo de explante y la concentración de ácido 2,4-diclorofenoxiacético en la formación de callos en Morus alba L. Effect of explant type and concentration of 2,4-dichlorophenoxyacetic acid on callus formation in Morus alba L.

    Directory of Open Access Journals (Sweden)

    A Espinosa

    2012-12-01

    Full Text Available El objetivo del trabajo fue evaluar el efecto del tipo de explante y la concentración de 2,4-D en la formación de callos, en Morus alba L. Como fuentes de explantes se utilizaron limbos foliares, tallos y pecíolos, obtenidos a partir de estacas que brotaron en condiciones de laboratorio. El medio de cultivo basal estuvo constituido por las sales y vitaminas MS (1962, con diferentes concentraciones de 2,4-D (0; 0,5; 1,0 y 2,0 mg.L-1. Se utilizó un diseño experimental completamente aleatorizado. La formación de callos en todos los tipos de explantes se inició en los primeros 15 días posteriores al establecimiento in vitro. La callogénesis solo ocurrió en los medios de cultivo con 2,4-D. La zona de formación del callo, su color y textura dependieron del tipo de explante utilizado. El aumento de la concentración de 2,4-D en el medio de cultivo incrementó el tamaño de los callos, con los mejores resultados en las concentraciones de 1,0 y 2,0 mg.L-1The objective of the work was to evaluate the effect of explant type and concentration of 2,4-dichlorophenoxyacetic acid on callus formation, in Morus alba L. As explant sources leaf blades, stems and petioles were used , obtained from cuttings which produced growths under laboratory conditions. The basal culture medium was constituted by MS salts and vitamins (1962, with different concentrations of 2,4-D (0; 0,5; 1,0 and 2,0 mg.L-1. A completely randomized experimental design was used. Callus formation in all explant types started in the first 15 days after the in vitro establishment. Callogenesis only occurred in the culture media with 2,4-D. The callus formation zone, callus color and texture depended on the explant type used. The increase of 2,4-D concentration in the culture medium increased callus size, with the best results in the concentrations 1,0 and 2,0 mg.L-1.

  1. Research Progress on Antioxidant Activity of Wood Vinegar and Extract from Morus alba Leaves%木醋液与桑叶提取物的抗氧化活性进展∗

    Institute of Scientific and Technical Information of China (English)

    纪蒙; 王海英; 施月园; 燕云飞; 刘一迪

    2016-01-01

    Determination of antibacterial activity and antioxidant activity of wood vinegar are theoretical basis for development and utilization of high value added wood vinegar products. Antioxidant activity of wood vinegar is different from that of raw materials, preparation conditions and refining methods. Flavonoid compounds and polysaccharide compounds from Morus alba leaves have strong hypoglycemic activity and antioxidant activity, respectively. Wood vinegar and Morus alba leaves extract compounding is expected to preparing synergistic antioxidant activity compound preparation, in order to get strong antioxidant activity of compound wood vinegar products.%木醋液的抑菌活性、抗氧化活性等测定为木醋液高附加值产品的开发利用提供理论基础。木醋液的抗氧化活性,因原料种类、制备条件、精制方法等而异。桑叶黄酮类化合物、多糖类化合物等均具有较强的降血糖活性和抗氧化活性。木醋液与桑叶提取物的复配,有望制备增效抗氧化活性复方制剂,以期得到抗氧化活性较强的复方木醋液产品。

  2. Multiplicación de propágulos infectivos HMA en una plantación de morera (Morus alba L.

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    Gertrudis Pentón

    Full Text Available Se realizó un ensayo de campo en la Estación Experimental de Pastos y Forrajes «Indio Hatuey», sobre un suelo Ferralítico Rojo de buen drenaje superficial e interno, con el objetivo de evaluar el efecto de la fertilización mineral y el intercalamiento de Canavalia ensiformis (canavalia inoculada con HMA, en la multiplicación de propágulos infectivos en una plantación de morera (Morus alba L.. El marco de siembra de la morera fue de 1,0 x 0,50 m (20 000 plantas/ha. El área de la plantación fue de 384 m2, con 40 plantas en las parcelas. El forraje se cosechó con un intervalo de 90 días, a una altura de 30 cm. Los esquemas evaluados fueron: testigo (sin fertilización y sin intercalamiento de canavalia, SFSCHMA; incorporación de HMA a través del intercalamiento de canavalia, sin fertilización mineral (SFCHMA; fertilización mineral (F e incorporación de HMA a través del intercalamiento de canavalia, con fertilización mineral (FCHMA. La canavalia se sembró inoculada con HMA a 0,5 m de la morera, en los primeros 15 días de crecimiento de esta a partir del corte, al inicio de cada época. El diseño fue de bloques al azar con arreglo factorial y tres repeticiones. Se evidenció un contenido bajo de propágulos infectivos nativos en la rizosfera de la plantación de morera (190-295 esporas/50 g. El efecto de FCHMA fue altamente significativo en la multiplicación de propágulos infectivos durante la época lluviosa y en los primeros 45 días de la poco lluviosa (370 y 469 esporas/50 g, respectivamente, mientras que propició aumentos de la materia orgánica y el K del suelo. Se concluye que la combinación de la fertilización mineral con la incorporación de HMA a través del intercalamiento de canavalia constituye una vía eficaz para multiplicar los propágulos infectivos HMA hasta niveles intermedios

  3. High throughput deep degradome sequencing reveals microRNAs and their targets in response to drought stress in mulberry (Morus alba)

    Science.gov (United States)

    Li, Ruixue; Chen, Dandan; Wang, Taichu; Wan, Yizhen; Li, Rongfang; Fang, Rongjun; Wang, Yuting; Hu, Fei; Zhou, Hong; Li, Long; Zhao, Weiguo

    2017-01-01

    MicroRNAs (miRNAs) play important regulatory roles by targeting mRNAs for cleavage or translational repression. Identification of miRNA targets is essential to better understanding the roles of miRNAs. miRNA targets have not been well characterized in mulberry (Morus alba). To anatomize miRNA guided gene regulation under drought stress, transcriptome-wide high throughput degradome sequencing was used in this study to directly detect drought stress responsive miRNA targets in mulberry. A drought library (DL) and a contrast library (CL) were constructed to capture the cleaved mRNAs for sequencing. In CL, 409 target genes of 30 conserved miRNA families and 990 target genes of 199 novel miRNAs were identified. In DL, 373 target genes of 30 conserved miRNA families and 950 target genes of 195 novel miRNAs were identified. Of the conserved miRNA families in DL, mno-miR156, mno-miR172, and mno-miR396 had the highest number of targets with 54, 52 and 41 transcripts, respectively, indicating that these three miRNA families and their target genes might play important functions in response to drought stress in mulberry. Additionally, we found that many of the target genes were transcription factors. By analyzing the miRNA-target molecular network, we found that the DL independent networks consisted of 838 miRNA-mRNA pairs (63.34%). The expression patterns of 11 target genes and 12 correspondent miRNAs were detected using qRT-PCR. Six miRNA targets were further verified by RNA ligase-mediated 5’ rapid amplification of cDNA ends (RLM-5’ RACE). Gene Ontology (GO) annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that these target transcripts were implicated in a broad range of biological processes and various metabolic pathways. This is the first study to comprehensively characterize target genes and their associated miRNAs in response to drought stress by degradome sequencing in mulberry. This study provides a framework for

  4. Producción de leche de una asociación de Leucaena leucocephala, Morus alba y Pennisetum purpureum CT-115 bajo condiciones de riego Milk production from an association of Leucaena leucocephala, Morus alba and Pennisetum purpureum CT-115 with irrigation

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    L Lamela

    2010-09-01

    Full Text Available En la vaquería "El Rancho"de la Empresa de Cítricos "Victoria de Girón" del municipio Jagüey Grande, en la provincia de Matanzas, Cuba, se realizó un estudio con el objetivo de determinar la producción de leche en una asociación de la gramínea Pennisetum purpureum CT-115 con los árboles forrajeros Leucaena leucocephala y Morus alba, en condiciones de riego. Se emplearon vacas de mediano potencial (Holstein x Cebú del grupo de alta producción, las cuales tuvieron acceso al sistema hasta los 150 días de lactancia. Se utilizó un modelo multiplicativo para analizar la producción de leche con efecto en la curva de lactancia; se analizaron las variables: bimestre de producción, época y número de lactancias, y se midió la disponibilidad de materia seca. La oferta de materia seca en el área de la asociación fue superior a 30 kg de MS/animal/rotación como promedio durante el período de evaluación, y hubo diferencias entre las épocas del año. Los mayores resultados se encontraron en el bimestre julio-agosto y los menores en enero-febrero y marzo-abril, con diferencias significativas (PIn the dairy unit "El Rancho" of the Citrus Fruit Firm "Victoria de Girón" of the Jagüey Grande municipality, in Matanzas province, Cuba, a study was conducted in order to determine milk production in an association of the grass Pennisetum purpureum CT-115 with the forage trees Leucaena leucocephala and Morus alba, with irrigation. Moderate-potential cows (Holstein x Zebu from the high-production group, which had access to the system until 150 days of lactation, were used. A multiplicative model was used to analyze the milk production with lactation curve effect; the following variables were analyzed: production two-month period, season and number of lactations; and dry matter availability was measured. The dry matter offer in the area of association was higher than 30 kg DM/animal/rotation as average during the evaluation period, and there were

  5. JNK1/2 Activation by an Extract from the Roots of Morus alba L. Reduces the Viability of Multidrug-Resistant MCF-7/Dox Cells by Inhibiting YB-1-Dependent MDR1 Expression

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    Youn Kyung Choi

    2013-01-01

    Full Text Available Cancer cells acquire anticancer drug resistance during chemotherapy, which aggravates cancer disease. MDR1 encoded from multidrug resistance gene 1 mainly causes multidrug resistance phenotypes of different cancer cells. In this study, we demonstrate that JNK1/2 activation by an extract from the root of Morus alba L. (White mulberry reduces doxorubicin-resistant MCF-7/Dox cell viability by inhibiting YB-1 regulation of MDR1 gene expression. When MCF-7 or MCF-7/Dox cells, where MDR1 is highly expressed were treated with an extract from roots or leaves of Morus alba L., respectively, the root extract from the mulberry (REM but not the leaf extract (LEM reduced cell viabilities of both MCF-7 and MCF-7/Dox cells, which was enhanced by cotreatment with doxorubicin. REM but not LEM further inhibited YB-1 nuclear translocation and its regulation of MDR1 gene expression. Moreover, REM promoted phosphorylation of c-Jun NH2-terminal kinase 1/2 (JNK1/2 and JNK1/2 inhibitor, SP600125 and rescued REM inhibition of both MDR1 expression and viabilities in MCF-7/Dox cells. Consistently, overexpression of JNK1, c-Jun, or c-Fos inhibited YB-1-dependent MDR1 expression and reduced viabilities in MCF-7/Dox cells. In conclusion, our data indicate that REM-activated JNK-cJun/c-Fos pathway decreases the viability of MCF-7/Dox cells by inhibiting YB-1-dependent MDR1 gene expression. Thus, we suggest that REM may be useful for treating multidrug-resistant cancer cells.

  6. Mulberry (Morus alba L.) Fruit Extract Containing Anthocyanins Improves Glycemic Control and Insulin Sensitivity via Activation of AMP-Activated Protein Kinase in Diabetic C57BL/Ksj-db/db Mice.

    Science.gov (United States)

    Choi, Kyung Ha; Lee, Hyun Ah; Park, Mi Hwa; Han, Ji-Sook

    2016-08-01

    The effect of mulberry (Morus alba L.) fruit extract (MFE) on hyperglycemia and insulin sensitivity in an animal model of type 2 diabetes was evaluated. C57BL/Ksj-diabetic db/db mice were divided into three groups: diabetic control, rosiglitazone, and MFE groups. Blood glucose, plasma insulin, and intraperitoneal glucose were measured, and an insulin tolerance test was performed after MFE supplementation in db/db mice. In addition, the protein levels of various targets of insulin signaling were measured by western blotting. The blood levels of glucose and HbA1c were significantly lower in the MFE-supplemented group than in the diabetic control group. Moreover, glucose and insulin tolerance tests showed that MFE treatment increased insulin sensitivity. The homeostatic index of insulin resistance significantly decreased in the MFE-supplemented group relative to the diabetic control group. MFE supplementation significantly stimulated the levels of phosphorylated (p)-AMP-activated protein kinase (pAMPK) and p-Akt substrate of 160 kDa (pAS160) and enhanced the level of plasma membrane-glucose transporter 4 (GLUT4) in skeletal muscles. Further, dietary MFE significantly increased pAMPK and decreased the levels of glucose 6-phosphatase and phosphoenolpyruvate carboxykinase in the liver. MFE may improve hyperglycemia and insulin sensitivity via activation of AMPK and AS160 in skeletal muscles and inhibition of gluconeogenesis in the liver.

  7. UP3005, a Botanical Composition Containing Two Standardized Extracts of Uncaria gambir and Morus alba, Improves Pain Sensitivity and Cartilage Degradations in Monosodium Iodoacetate-Induced Rat OA Disease Model

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    Mesfin Yimam

    2015-01-01

    Full Text Available Osteoarthritis (OA is a multifactorial disease primarily noted by cartilage degradation in association with inflammation that causes significant morbidity, joint pain, stiffness, and limited mobility. Present-day management of OA is inadequate due to the lack of principal therapies proven to be effective in hindering disease progression where symptomatic therapy focused approach masks the actual etiology leading to irreversible damage. Here, we describe the effect of UP3005, a composition containing a proprietary blend of two standardized extracts from the leaf of Uncaria gambir and the root bark of Morus alba, in maintaining joint structural integrity and alleviating OA associated symptoms in monosodium-iodoacetate- (MIA- induced rat OA disease model. Pain sensitivity, micro-CT, histopathology, and glycosaminoglycans (GAGs level analysis were conducted. Diclofenac at 10 mg/kg was used as a reference compound. UP3005 resulted in almost a complete inhibition in proteoglycans degradation, reductions of 16.6% (week 4, 40.5% (week 5, and 22.0% (week 6 in pain sensitivity, statistically significant improvements in articular cartilage matrix integrity, minimal visual subchondral bone damage, and statistically significant increase in bone mineral density when compared to the vehicle control with MIA. Therefore, UP3005 could potentially be considered as an alternative therapy from natural sources for the treatment of OA and/or its associated symptoms.

  8. Enhanced anti-immobility effects of Sanggenon G isolated from the root bark of Morus alba combined with the α2-antagonist yohimbine in the rat forced swim test.

    Science.gov (United States)

    Lim, Dong Wook; Baek, Nam-In; Kim, Yun Tai; Lee, Changho; Kim, In-Ho; Han, Daeseok

    2016-07-01

    In this study, we aimed to determine whether Sanggenon G, an active compound isolated from the root bark of Morus alba, exhibited enhanced anti-immobility activity with the addition of the α2-antagonist yohimbine in rats subjected to forced swim test (FST)-induced depression. Fluoxetine (a selective serotonin reuptake inhibitor) treatment in rats reduced the immobility time, and pretreatment with yohimbine significantly enhanced the antidepressant-like behavior of fluoxetine at 5, 10 and 20 mg/kg. Similarly, Sanggenon G significantly decreased the immobility time, reducing immobility by a maximum of 43.9 % when treated at a dose of 20 mg/kg. Furthermore, pretreatment with yohimbine significantly enhanced the antidepressant-like behavior of Sanggenon G at 5 and 10 mg/kg. Our findings suggest that the antidepressant-like effect of Sanggenon G could be facilitated by concomitant use of the α2-antagonist. Further studies are needed to evaluate the potential of Sanggenon G as an alternative therapeutic approach for the treatment of depression.

  9. 桑叶多糖调节小鼠肠道菌群失调的研究%Regulation ofMorus alba polysaccharides on disturbance of intestinal flora in mice

    Institute of Scientific and Technical Information of China (English)

    陈涟昊; 张霞; 孙世芳; 王菱枝; 车有; 赵骏; 宋存江

    2015-01-01

    目的:研究桑叶多糖对小鼠肠道菌群失调的调节作用。方法利用盐酸林可霉素诱导小鼠肠道菌群失调。将造模成功的小鼠随机分为桑叶多糖组、乳酶生组、模型组,另设置对照组。桑叶多糖组小鼠ig 6.0 mg/mL桑叶多糖溶液0.6 mL(剂量0.144 mg/g),乳酶生组小鼠ig 5.5 mg/mL乳酶生混悬液0.6 mL(剂量0.132 mg/g)。2次/d,连续给药7 d。模型组、对照组小鼠自行饮水。取小鼠的新鲜粪便,提取肠道菌DNA。16S段DNA序列扩增,变性梯度凝胶电泳(DGGE)法分析。比对各条带及样品,进行聚类分析,对各组小鼠肠道微生物群落结构进行主成分分析。结果 DGGE直观图分析,经过自然恢复、桑叶多糖和乳酶生的作用,小鼠肠道菌群种类有了明显的增加,但与对照组小鼠的肠道菌群比较仍然有差别,并不能完全恢复到原来的水平。聚类分析结果可以看出,与对照组最接近的是乳酶生组,其次是桑叶多糖组,最后是自然恢复组。菌群落结构的主成分分析可以直观地看出,桑叶多糖、乳酶生组小鼠肠道菌群与对照组关系最近,模型组与对照组肠道菌群及其他组肠道菌群的差异明显。结论桑叶多糖对小鼠肠道菌群失调有调节作用。%Objective To study the regulation ofMorus alba polysaccharides on disturbance of intestinal flora in mice.Methods Models of disturbance of intestinal flora in mice were induced by lincomycin hydrochloride. Mice with disturbance of intestinal flora were randomly divided intoM. alba polysaccharides group, lactasin group, and model group. And other rats were selected as the control group. The mice in the M. alba polysaccharides group were ig administered with 6.0 mg/mLM. alba polysaccharides 0.6 mL (equivalent to dosage 0.144 mg/g), and those in lactasin group were ig administered with 5.5 mg/mL Lactasin Tablets 0.6 mL (equivalent to dosage 0.132 mg/g). The

  10. The Effects of Supplementary Mulberry Leaf (Morus alba) Extracts on the Trace Element Status (Fe, Zn and Cu) in Relation to Diabetes Management and Antioxidant Indices in Diabetic Rats.

    Science.gov (United States)

    Król, Ewelina; Jeszka-Skowron, Magdalena; Krejpcio, Zbigniew; Flaczyk, Ewa; Wójciak, Rafał W

    2016-11-01

    Mulberry leaves (Morus alba) have been used in folk medicine to mitigate symptoms of diabetes. The mulberry plant contains phenolic compounds that are able to decrease blood glucose concentration. Since various phenolics have antioxidant and metal binding properties, they can be used to alleviate oxidative stress and chelate trace elements involved in redox reactions. The aim of this study was to evaluate the effects of dietary supplementation with mulberry leaf extracts (acetone-water (AE) and ethanol-water (EE)) on the trace element status (Fe, Zn and Cu) in relation to diabetes management and antioxidant indices in high-fat diet-fed/STZ diabetic rats. The experiment was performed on 38 male Wistar rats with diabetes (induced by high-fat diet (HF) and streptozotocin injection) or the control fed with AIN-93M or high-fat diet. As a result, five experimental groups were used: (1) a healthy control group fed with AIN-93M; (2) an HF control group; (3) a diabetic HF group; (4) a diabetic HF + AE group (6 g/kg diet); (5) a diabetic HF + EE group (6 g/kg diet). The rats were fed with appropriate diets for 4 weeks. The content of trace elements (Fe, Zn and Cu) in the serum and tissues was measured by means of atomic absorption spectrometry (AAS). Biochemical analyses (glucose, TBARS, FRAP) were performed on the blood serum. It was shown that the AE decreased hepatic and renal Fe stores, while the EE increased hepatic Cu levels in diabetic rats and confirmed their ability to regulate the Fe and Cu status in diabetes. The results confirmed a significant hypoglycaemic and antioxidant potential of both mulberry leaf extracts in diabetic rats.

  11. Molecular cloning and characterization of novel Morus alba germin-like protein gene which encodes for a silkworm gut digestion-resistant antimicrobial protein.

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    Bharat Bhusan Patnaik

    Full Text Available BACKGROUND: Silkworm fecal matter is considered one of the richest sources of antimicrobial and antiviral protein (substances and such economically feasible and eco-friendly proteins acting as secondary metabolites from the insect system can be explored for their practical utility in conferring broad spectrum disease resistance against pathogenic microbial specimens. METHODOLOGY/PRINCIPAL FINDINGS: Silkworm fecal matter extracts prepared in 0.02 M phosphate buffer saline (pH 7.4, at a temperature of 60°C was subjected to 40% saturated ammonium sulphate precipitation and purified by gel-filtration chromatography (GFC. SDS-PAGE under denaturing conditions showed a single band at about 21.5 kDa. The peak fraction, thus obtained by GFC wastested for homogeneityusing C18reverse-phase high performance liquid chromatography (HPLC. The activity of the purified protein was tested against selected Gram +/- bacteria and phytopathogenic Fusarium species with concentration-dependent inhibitionrelationship. The purified bioactive protein was subjected to matrix-assisted laser desorption and ionization-time of flight mass spectrometry (MALDI-TOF-MS and N-terminal sequencing by Edman degradation towards its identification. The N-terminal first 18 amino acid sequence following the predicted signal peptide showed homology to plant germin-like proteins (Glp. In order to characterize the full-length gene sequence in detail, the partial cDNA was cloned and sequenced using degenerate primers, followed by 5'- and 3'-rapid amplification of cDNA ends (RACE-PCR. The full-length cDNA sequence composed of 630 bp encoding 209 amino acids and corresponded to germin-like proteins (Glps involved in plant development and defense. CONCLUSIONS/SIGNIFICANCE: The study reports, characterization of novel Glpbelonging to subfamily 3 from M. alba by the purification of mature active protein from silkworm fecal matter. The N-terminal amino acid sequence of the purified protein was

  12. 桑树脱落酸生物合成相关基因的鉴定及转录表达分析%Characterization and Transcriptional Expression Analysis of ABA Biosynthesis Related Genes from Mulberry (Morus albaL.)

    Institute of Scientific and Technical Information of China (English)

    朱攀攀; 余茂德; 刘长英; 赵爱春; 裴汭超; 李军; 王晓红; 李镇刚; 王茜龄; 鲁成

    2015-01-01

    被下调,MnAAO和MnZEP1只在第4天升高,MnZEP1只在处理后第1天被下调,MnNCED2在ABA和氟啶酮处理后均被下调。【结论】从桑树中获得了6个ABA合成相关基因MnAAO、MnZEP1—MnZEP2和NCED1—NcED3,MnNCEDs在果实发育中后期的转录表达相对较高,并与ABA的含量变化相一致,可能对ABA的合成起主要调控作用,外源ABA处理能够促进桑椹的成熟和脱落,氟啶酮处理能够抑制桑椹的成熟和脱落。%[Objective] 9-cisepoxycarotenoid dioxygenase (NCED), ABA-aldehyde oxidase(AAO), Zeaxanthinepoxidase (ZEP) are the key enzymes involved in biosynthesis of abscisic acid (ABA) via indirect pathway. The aim of this study was to analyze the transcriptional expression of ABA biosynthesis related genes and detect the content of ABA during Jialing 40 (Morus atropurpurea Roxb.) fruit development. The effects of ABA and its synthesis inhibitors on fruit development were also explored. This work will lay a foundation for further studying the role of ABA on mulberry (Morus alba L.) fruit maturity and senescence.[Method]In this study, the sequences of six putative ABA biosynthesis related genes were obtained fromMorusnotabilis genome database and its deduced amino acid sequences were analyzed by using bioinformatics tools. Total RNA was extracted from mulberry fruit using RNAiso Plus (TaKaRa) and reverse transcribed to synthesize cDNA. The relative transcriptional expression of ABA biosynthesis related genes in different developmental stages and treated mulberry fruits was investigated by using qRT-PCR. The content of ABA was determined by using HPLC.[Result]Six ABA biosynthesis related genes were isolated from mulberry, including one AAO gene, twoZEP genes and three NCED genes. Multiple-sequence alignment results showed thatMnNCEDs amino acids are relatively conserved in higher plants. Cluster analysis revealed that MnNCED1-3 are closely related with dicotyledons plants and farther with monocotyledons plants. Transcription

  13. INCLUSIÓN DE HARINAS DE FOLLAJES ARBÓREOS Y ARBUSTIVOS TROPICALES (Morus alba, Erythrina poeppigiana, Tithonia diversifolia EHibiscus rosa-sinensis EN LA ALIMENTACIÓN DE CUYES (Cavia porcellus Linnaeus

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    G. A. Meza

    2014-01-01

    Full Text Available La investigación se ejecutó en la Finca Experimental “La María” propiedad de la Univer-sidad Técnica Estatal de Quevedo (UTEQ localizada en el km 71/2de la vía Quevedo-Mocache; Provincia de Los Ríos cuya ubicación geográfica de 1º 6’ 23” de latitud sury 79º 29’ 12” de longitud oeste y a una altura de 73 m.s.n.m. El objetivo principal fueevaluar el comportamiento productivo de cuyes con la inclusión del 20% de harinasderivadas de follajes arbustivos y arbóreos tropicales. Se utilizaron 40 cuyes macho de 30días de edad. Se empleó un diseño completamente al azar con cinco tratamientos, cuatroréplicas y la unidad experimental estuvo conformado por dos cuyes. Para determinarlas diferencias entre medias de tratamientos se aplicó la prueba de Tukey (P≤0.05. Seevaluaron cinco dietas-tratamientos: (T0 dieta 100% balanceado, (T1 80% dieta y 20%harina deMorus alba, (T2 80% dieta y 20% harina deErythrina poeppigiana, (T3 80%dieta y 20% harina deTithonia diversifolia, (T4 80% dieta y 20% harina deHibiscusrosa-sinensis. Las variables bajo estudio fueron: consumo de alimento de balanceado enmateria seca (CABMS, g, ganancia de peso (GP, g, índice de conversión alimenticia(ICA y rendimiento en canal (RC, %. La rentabilidad de los tratamientos se determinóa través de la relación beneficio-costo (R b/c. Los mayores (P<0.01 CABMS, GP-ICAy RC-Rentabilidad, la registraron los tratamientos: T0 (48.34 g MS animal-1d-1, T1(8.80 g animal-1d-1y 5.04 y el T3 (77.67% y 26.20%, respectivamente.

  14. Morus alba L. nature's functional tonic

    NARCIS (Netherlands)

    Butt, M.S.; Nazir, A.; Tauseef Sultan, M.; Schroën, C.G.P.H.

    2008-01-01

    Currently, importance of natural products is being revitalized to alleviate various health discrepancies. The link between health and diet is well documented and the consumers' trend reflects conscience towards their dietary habits. Probing these links has led to the emergence of functional,

  15. Morus alba L. nature's functional tonic

    NARCIS (Netherlands)

    Butt, M.S.; Nazir, A.; Tauseef Sultan, M.; Schroën, C.G.P.H.

    2008-01-01

    Currently, importance of natural products is being revitalized to alleviate various health discrepancies. The link between health and diet is well documented and the consumers' trend reflects conscience towards their dietary habits. Probing these links has led to the emergence of functional, nutrace

  16. Free radical scavenging activity, total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae extracts

    Directory of Open Access Journals (Sweden)

    Radojković Marija M.

    2012-01-01

    Full Text Available Mulberry (Morus spp. L., Moraceae fruits, leaves, bark and branch have been used in traditional medicine as diuretic, hypoglycemic and hypotensive. The mechanism of their effects is correlated with the content of active components. Objective of this work was to evaluate and compare antioxidant properties of different extracts of two Morus species growing in Serbia: Morus alba L. (white mulberry and Morus nigra L. (black mulberry. Potential antioxidant activity, content of antioxidant compounds (phenolics and flavonoids and radical scavenging capacity, tested by DPPH method, were evaluated. The phenolic and flavonoid composition of different Morus extracts was determined by the HPLC method. The extracts prepared from fruits, leaves and roots of M. alba and M. nigra exhibited different characteristics. The highest extraction yield was achieved by M. alba leaves extraction (23.40%. M. nigra roots extract shown the highest total phenolics (186.30 mg CAE/g, while highest total flavonoids content (67.37 mg RE/g was determined for M. nigra leaves extracts. In addition, black mulberry leaves extracts with the highest antioxidant activity had the highest phenolic acids contents. The dominant phenolic components in the samples were rutin and chlorogenic acid. All investigated mulberry dry extracts shown high content of phenolic compounds and significant antioxidant activity. This work contributes to knowledge of the antioxidant properties of Morus species. The obtained results may be useful in the evaluation of new dietary supplements and food products.

  17. Definition of Eight Mulberry Species in the Genus Morus by Internal Transcribed Spacer-Based Phylogeny.

    Science.gov (United States)

    Zeng, Qiwei; Chen, Hongyu; Zhang, Chao; Han, Minjing; Li, Tian; Qi, Xiwu; Xiang, Zhonghuai; He, Ningjia

    2015-01-01

    Mulberry, belonging to the order Rosales, family Moraceae, and genus Morus, has received attention because of both its economic and medicinal value, as well as for its important ecological function. The genus Morus has a worldwide distribution, however, its taxonomy remains complex and disputed. Many studies have attempted to classify Morus species, resulting in varied numbers of designated Morus spp. To address this issue, we used information from internal transcribed spacer (ITS) genetic sequences to study the taxonomy of all the members of generally accepted genus Morus. We found that intraspecific 5.8S rRNA sequences were identical but that interspecific 5.8S sequences were diverse. M. alba and M. notabilis showed the shortest (215 bp) and the longest (233 bp) ITS1 sequence length, respectively. With the completion of the mulberry genome, we could identify single nucleotide polymorphisms within the ITS locus in the M. notabilis genome. From reconstruction of a phylogenetic tree based on the complete ITS data, we propose that the Morus genus should be classified into eight species, including M. alba, M. nigra, M. notabilis, M. serrata, M. celtidifolia, M. insignis, M. rubra, and M. mesozygia. Furthermore, the classification of the ITS sequences of known interspecific hybrid clones into both paternal and maternal clades indicated that ITS variation was sufficient to distinguish interspecific hybrids in the genus Morus.

  18. Definition of Eight Mulberry Species in the Genus Morus by Internal Transcribed Spacer-Based Phylogeny.

    Directory of Open Access Journals (Sweden)

    Qiwei Zeng

    Full Text Available Mulberry, belonging to the order Rosales, family Moraceae, and genus Morus, has received attention because of both its economic and medicinal value, as well as for its important ecological function. The genus Morus has a worldwide distribution, however, its taxonomy remains complex and disputed. Many studies have attempted to classify Morus species, resulting in varied numbers of designated Morus spp. To address this issue, we used information from internal transcribed spacer (ITS genetic sequences to study the taxonomy of all the members of generally accepted genus Morus. We found that intraspecific 5.8S rRNA sequences were identical but that interspecific 5.8S sequences were diverse. M. alba and M. notabilis showed the shortest (215 bp and the longest (233 bp ITS1 sequence length, respectively. With the completion of the mulberry genome, we could identify single nucleotide polymorphisms within the ITS locus in the M. notabilis genome. From reconstruction of a phylogenetic tree based on the complete ITS data, we propose that the Morus genus should be classified into eight species, including M. alba, M. nigra, M. notabilis, M. serrata, M. celtidifolia, M. insignis, M. rubra, and M. mesozygia. Furthermore, the classification of the ITS sequences of known interspecific hybrid clones into both paternal and maternal clades indicated that ITS variation was sufficient to distinguish interspecific hybrids in the genus Morus.

  19. Controle de Qualidade e triagem fitoquímica da droga vegetal das folhas de Morus nigra L. (MORACEAE)

    OpenAIRE

    Pedro Luis Guizzo; Thaís Cristina Cuba Bredda; Maria Virgínia Costa Scarpa; Fernanda Flores Navarro

    2015-01-01

    Uma rigorosa análise de controle de qualidade é uma das etapas na produção de fitoterápicos. Devido a escassez de estudos sobre Morus nigra L. (MORACEAE), mais conhecida como amora, este trabalho teve como objetivo o controle de qualidade das folhas da amoreira, incluindo uma análise Fitoquímica preliminar, controle de qualidade físico-químico e microbiológico utilizando metodologias farmacopeicas e não farmacopeicas. Os testes fitoquímicos evidenciaram a presença de isoflavonas, taninos hidr...

  20. Quantitative analysis of oxyresveratrol in different plant parts of Morus species and related Genera by HPTLC

    Science.gov (United States)

    Four aromatic compounds; oxyresveratrol (1), mulberroside A (2), cudraflavone C (3) and kuwanone J (4) were isolated from the stems of Morus rubra L. The quantitative determination of oxyresveratrol from M. rubra L., M. alba L. and related genera by high performance thin layer chromatography (HPTLC)...

  1. Efeito, no primeiro ano agrícola, da época de poda e do espaçamento sôbre a produção de fôlhas de amoreira, de diferentes variedades, formadas pelo sistema de cepo: parte I - estudo na região de Pindorama Effect of pruning season and spacing stated in the first crop season upon leaf production on the mulberry trees of different varieties shaped by the "cepo" system: part 1 - studies in the Pindorama region

    Directory of Open Access Journals (Sweden)

    António Castilho Rúbia

    1966-01-01

    Full Text Available São apresentados os resultados da produção de fôlhas de três variedades comuns de amoreira: Calabreza, Fernão Dias e Lopes Lins, conduzidas no sistema de formação em cepo, com mudas plantadas em diferentes espaçamentos e épocas de poda de produção. No primeiro ano agrícola, as melhores produções foram obtidas com a variedade Calabreza, no espaçamento de 1,50 X 1,00 m e com a época de poda mais atrazada, de 20 de julho.Preliminary results are presented in this paper of an experiment with Morus albaL. designed for the purpose of leaf production and laid out as a factorial 3³ experiment (Cochran - 2 replications, in which were studied three common varieties of mulberry trees : Calabreza, Fernão Dias and Lopes Lins, same being grown and shaped through the "cepo" system and planted in the three spacings 1.5 by 1.0, 1.5 by 1.5 and 1.5 by 2.0 meters, the pruning time having been in three different seasons, i. e., June 10, June 30 and July 20. The first crop year (1964/1965 proved that this trial, installed at the Experiment Station of Pindorama, of the Instituto Agronômico, showed the best production of leaves obtained with the variety Calabreza, when using the 1.5 X 1.0 m spacing and the latest pruning season, namely July 20.

  2. 荧光衍生化法测定不同季节桑叶中1-脱氧野尻霉素的含量%Determination of 1-deoxynojirimycin in Morus alba L.leaves of different seasons by fluorometric derivatization

    Institute of Scientific and Technical Information of China (English)

    李继文; 郭晓燕; 王腾; 李翠清

    2011-01-01

    Objective:To develop a high performance liquid chromatographic method for the determination of 1-deoxynojirimycin(DNJ)in Morus alba L. leaves and contrast the content of 1-DNJ in different seasons. Methods:The separation was performed with a Kromasil C18 column(4. 6mm×250mm,5μm)at 30℃ and the mobile phase consisted of acetonitrile-0. 1%aqueous acetic acid(50∶ 50),at the rate of 1. 0mL/min. The 1-DNJ marked with 9-fluorenylmethyl chloroformate(FMOC-Cl)was detected at excitation wavelength of 254nm and emission wavelength of 322nm. Results:The calibration curve was linear over the concentration range from 10mg/L to 35mg/L(r=0. 99959),the limit of detection was 0. 80mg/L(S/N=3)and the average recovery was 95. 38%(RSD=2. 28%). The results showed that the content of 1-DNJ was significantly different in different seasons. Conclusion:This method was simple,rapid,and accurate and was suitable for the determination of 1-DNJ in Morus alba L. leaves.%目的:建立测定桑叶中1-脱氧野尻霉素(1-DNJ)含量的方法,并比较不同季节桑叶中1-DNJ的含量。方法:采用高效液相色谱法测定,以芴甲氧羰酰氯(FMOC-Cl)为柱前衍生化试剂,对衍生化条件进行了优化;色谱柱为Kromasil C18柱(4.6mm×250mm,5μm),流动相为乙腈-0.1%醋酸(50∶50),流速1.0mL/min,柱温30℃,荧光检测器激发波长254nm,发射波长322nm。结果:1-DNJ在10~35mg/L范围内线性良好(r=0.99959),检测限为0.80mg/L(S/N=3),平均回收率为95.38%,相对标准偏差2.28%(n=5)。桑叶中1-DNJ的含量在不同生长季节间存在显著差异。结论:方法简便、快速、准确,适用于桑叶中1-DNJ含量的定量分析。

  3. Сравнительное изучение аминокислотного состава листьев шелковицы черной (Morus nigra L. ), шелковицы белой (Morus alba L. ) и шелковицы красной (Morus rubra L. )

    OpenAIRE

    Селина, И.

    2014-01-01

    Изучен качественный и количественный состав аминокислот листьев шелковицы черной (Morus nigra L.), шелковицы белой (Morus alba L.) и шелковицы красной (Morus rubra L.). Методом тонкослойной хроматографии (ТСХ) с использованием стандартных образцов аминокислот осуществлен предварительный анализ содержания свободных аминокислот. Установлено, что в исследуемых образцах идентифицированы глицин, глутаминовая кислота, метионин, тирозин. Дальнейшее определение суммы аминокислот определяли на аминоки...

  4. Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.)%桑树MaACS和MaACO基因的鉴定和表达模式研究

    Institute of Scientific and Technical Information of China (English)

    Chang-ying LIU; Shu-mei HAN; Cheng LU; Mao-de YU; Rui-hua LÜ; Jun LI; Ai-chun ZHAO; Xi-ling WANG; Umuhoza DIANE; Xiao-hong WANG; Chuan-hong WANG; Ya-sheng YU

    2014-01-01

    研究目的:分离和鉴定桑树中参与乙烯生物合成的酶的编码基因MaACS 和MaACO,研究其表达模式。创新要点:基于最新公布的桑树基因组数据库数据,获得5个MaACS 基因和2个MaACO 基因,对其进行了生物信息分析,同时鉴定了其在不同桑树组织中、不同发育时期桑椹中和不同激素作用下的表达模式。研究方法:通过生物信息学方法筛选和鉴定基因,利用荧光定量逆转录聚合酶链式反应(qRT-PCR)分析基因的表达量。重要结论:MaACS 和MaACO 基因在根、茎、叶等不同组织中呈现出不同的表达模式,在桑椹发育过程中呈现出两种表达模式,其表达量被脱落酸和乙烯利上调。%1-Aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type I, MaACS3 and MaACS4 are type II, and MaACS5 is type III, with different C-terminal se-quences. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demon-strated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid (ABA) and ethephon.

  5. 桑树花青素合酶基因的克隆与信息学分析%Molecular Cloning and Information Analysis of ANS Genes Encoding Anthocyanin Synthases from Mulberry (Morus alba)

    Institute of Scientific and Technical Information of China (English)

    张琼予; 李军; 赵爱春; 王茜龄; 金筱耘; 李镇刚; 余茂德

    2012-01-01

    花青素合酶ANS是花青素合成过程中的一个关键限速酶.本文利用同源克隆、RT-PCR从桑椹中克隆出花青素合酶(MaANS)基因,并进行生物信息学分析和组织特异性表达分析.通过染色体步移法获得的MaANS基因的5’端和3’端,获得基因全长1 535 bp,由2个外显子和1个内含子组成,包含完整CDS区域为1 077 bp,编码358个氨基酸,与来源于草莓、甘薯、苹果、沙梨的ANS基因同源性均达到80%以上.MaANS编码的蛋白质属于2-酮戊二酸双加氧酶家族.MaANS在进化树中的位置与草莓最近,与苹果、沙梨次之.组织表达分析表明,MaANS基因在幼叶和成熟果实中高水平表达,表明该基因的表达具有组织特异性,为研究桑树果色的形成原因和表达调控奠定了基础.%Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase) is one of the critical enzymes in the biosynthesis of the anthocyanin. Anthocyanidin synthase gene fragment (designated as MaANS) was isolated from mulberry fruit (Mom alba) by RT-PCR based on homology cloning and genome walking technology. MaANS with the 5' and 3' was cloned by genome-walking. The full-length genomic sequence of MaANS is 1 535 bp, which consists of two exons and one intron. The coding region length was 1 077 bp, and their deduced protein consisted of 358 amino acid residues. Multiple alignments revealed that the nucleic acid of MaANS shared above 80% identity with that of Fragaria x ananassa, Ipomoea batatas, Mains domestica, and Pyrus pyrifolia. Structural analysis showed that the MaANS protein might belong to the 2 OG and Fe(H)-dependent oxygenase family. Phyloge-netic tree analysis revealed that MaANS was the most close with Fragaria x ananassa, then Malus domestica and Pyrus pyrifolia. Reverse transcription-PCR (RT-PCR) analyses of MaANS transcripts showed that it was abundantly expressed in the young leaves and ripened fruit. All research made an essential foundation for pathway and

  6. CONSUMO E UTILIZAÇÃO DO ALIMENTO PELO BICHO-DA-SEDA (Bombyx mori L., ALIMENTADO COM DOIS CULTIVARES DE AMOREIRA EM DIFERENTES IDADES DE CORTE

    Directory of Open Access Journals (Sweden)

    Sílvia Maria Alves Gomes Dierckx

    2006-10-01

    Full Text Available O presente estudo foi desenvolvido na Unidade de Pesquisa e Desenvolvimento de Gália-SP, da APTA/SAA, no período de 31 de dezembro de 1997 a 29 de abril de 1998. Teve por objetivo avaliar, através de índices nutricionais, os efeitos da idade de corte (7, 10, 13 e 16 semanas de dois cultivares de amoreira (IZ 56/4 e Korin sobre o consumo e utilização do alimento pelo bicho-da-seda (Bombyx mori L..Com dez semanas de desenvolvimento o cultivar IZ 56/4 apresentou menor consumo e maior eficiência de utilização pelas lagartas no quinto ínstar, tendência também observada para o cultivar Korin. No geral, o cultivar Korin proporcionou as melhores características nutricionais, com menor ingestão, menor custo metabólico, maior eficiência de conversão e boa taxa de crescimento e digestibilidade. As lagartas do bicho-da-seda apresentaram boa capacidade compensatória em condições nutricionais inadequadas, de forma a manter um ótimo crescimento e desenvolvimento. PALAVRAS-CHAVE: Bicho-da-seda, idade de corte, Morus sp.

  7. Controle de Qualidade e triagem fitoquímica da droga vegetal das folhas de Morus nigra L. (MORACEAE

    Directory of Open Access Journals (Sweden)

    Pedro Luis Guizzo

    2015-10-01

    Full Text Available Uma rigorosa análise de controle de qualidade é uma das etapas na produção de fitoterápicos. Devido a escassez de estudos sobre Morus nigra L. (MORACEAE, mais conhecida como amora, este trabalho teve como objetivo o controle de qualidade das folhas da amoreira, incluindo uma análise Fitoquímica preliminar, controle de qualidade físico-químico e microbiológico utilizando metodologias farmacopeicas e não farmacopeicas. Os testes fitoquímicos evidenciaram a presença de isoflavonas, taninos hidrolisáveis e alcaloides. Os resultados do controle físico-químico e microbiológico mostraram-se de acordo com as especificações. Isso destaca a importância do estabelecimento de normas para o controle da qualidade para as plantas, a fim de que sejam utilizadas como fitoterápicos.Palavras-chave: Controle de qualidade. Droga vegetal. Fitoquímica. Morus nigra. ABSTRACT A rigorous quality control analysis is one of the steps in the production of herbal medicines. Due to lack of studies on Morus nigra L. (Moraceae, better known as mulberry, this study had as objective the quality control of mulberry leaves, including a preliminary Phytochemical analysis of physical-chemical and microbiological quality control methodologies, using pharmacopoeic and non pharmacopoeic. Phytochemicals tests revealed the presence of isoflavones, hydrolysable tannins and alkaloids. The results of the physic-chemical and microbiological control shown in accordance with the specifications. This shows the importance of establishing standards for quality control for plants, to be used as herbal medicines.Keywords: Quality control. vegetal drug. Phytochemistry. Morus nigra.

  8. The antiproliferative effect of mulberry (Morus alba L.) plant on ...

    African Journals Online (AJOL)

    Shadia A. Fathy

    2013-08-12

    Aug 12, 2013 ... well-differentiated transformed cell line closely related to. HCC. 2. Materials ... methanol (MeOH) high-performance liquid chromatography. HPLC grade ... chased from the holding company for biological products. (VACSERA); in a .... might constitute an effective strategy to be a preferential meth- od for the ...

  9. Chemical composition and antioxidant activity of certain Morus species.

    Science.gov (United States)

    Imran, Mohammad; Khan, Hamayun; Shah, Mohibullah; Khan, Rasool; Khan, Faridullah

    2010-12-01

    In the present work, the fruits of four Morus species, namely Morus alba (white mulberry), Morus nigra (black mulberry), Morus laevigata (large white fruit), and Morus laevigata (large black fruit), were analyzed for proximate composition, essential minerals, and antioxidant potentials. For this purpose, the ripe fruits were collected from the northern regions of Pakistan. The major nutritional components (moisture, ash, lipids, proteins, fibres, carbohydrates, and total sugar) were found to be in the suitable range along with good computed energy. Total dry weight, pH, and titratable acidity (percent citric acid) were (17.60±1.94)-(21.97±2.34) mg/100 g, (3.20±0.07)-(4.78±0.15), and (0.84±0.40)%-(2.00±0.08)%, respectively. Low riboflavin (vitamin B(2)) and niacin (vitamin B(3)) contents were recorded in all the fruits, while ascorbic acid (vitamin C) was in the range from (15.20±1.25) to (17.03±1.71) mg/100 g fresh weight (FW). The mulberry fruits were rich with regard to the total phenol and alkaloid contents, having values of (880±7.20)-(1650±12.25) mg/100 g FW and (390±3.22)-(660±5.25) mg/100 g FW, respectively. Sufficient quantities of essential macro-(K, Ca, Mg, and Na) and micro-(Fe, Zn, and Ni) elements were found in all the fruits. K was the predominant element with concentration ranging from (1270±9.36) to (1731±11.50) mg/100 g, while Ca, Na, and Mg contents were (440±3.21)-(576±7.37), (260±3.86)-(280±3.50), and (240±3.51)-(360±4.20) mg/100 g, respectivly. The decreasing order of micro-minerals was Fe>Zn>Ni. The radical scavenging activity of methanolic extract of fruits was concentration-dependent and showed a correlation with total phenolic constituents of the respective fruits. Based on the results obtained, mulberry fruits were found to serve as a potential source of food diet and natural antioxidants.

  10. Characterization of Morus species in respect to micro, macro, and toxic elements

    Directory of Open Access Journals (Sweden)

    Radojković Marija M.

    2014-01-01

    Full Text Available This study examines the mineral composition of the extracts of the fruits, leaves and roots of white mulberry (Morus alba L. and black mulberry (Morus nigra L. grown in Serbia. All extract samples of white and black mulberry were analyzed for the content of micro (B, Co, Cr, Cu, Fe, Li, Mn, Ni, Se, Sr, Zn, macro (Ca, Mg, Na, and toxic metals (Al, As, Cd, Hg, Pb by inductively coupled plasma optical emission spectrometry (ICP-OES. The study revealed that parts of the plant had statistically significant impact on the levels of the examined elements among the two Morus species. All extracts contained high amounts of Ca, Mg, Na, B, Cu, Fe, Mn and Zn. The studies showed that in the most of extracts dominant macro element was Mg (591- 1942 μg/g of dry extract, while dominant microelements were Zn, B, Cu in all extracts, except for the black mulberry leaves, whose extract was most abundant in Fe (143 μg/g of dry extract. The highest content of micro and macro elements was detected in the extract of black mulberry leaves. This work contributes to the knowledge of the nutritional properties of Morus species. The obtained results may be useful in the evaluation of new dietary and food products.[ Projekat Ministarstva nauke Republike Srbije, br. TR 31013

  11. Phytochemical profiles of different mulberry (Morus sp.) species from China.

    Science.gov (United States)

    Song, Wei; Wang, Han-Jing; Bucheli, Peter; Zhang, Pei-Fang; Wei, Dong-Zhi; Lu, Yan-Hua

    2009-10-14

    Mulberry is rich in alkaloids, polyphenols, flavonoids, and anthocyanins, which have been suggested to be responsible for health benefits. The concentrations of 1-deoxynojirimycin (DNJ), resveratrol, oxyresveratrol, cyanidin-3-O-beta-glucoside (Cy-3-glu), cyanidin-3-O-beta-rutinoside (Cy-3-rut), and rutin in mulberry juice, fruits, and leaves of 8 species grown in China were examined. It is the first time content determination of DNJ in mulberry juice and oxyresveratrol in mulberry fruits and leaves has been reported. Among the varieties tested, Da 10 (Morus atropurpurea Roxb.) was the most valuable cultivar considering its high content of functional components. Besides, Hetianbaisang (M. alba Linn.), Taiwanguosang (M. atropurpurea Roxb.), Fujian 2 hao (M. alba Linn.), Gaozhoujisang (M. australis Poir.), and Shanxiguosang (M. nigra Linn.) were rich in DNJ, resveratrol, oxyresveratrol, anthocyanins, and flavonoids, respectively. The high contents of functional compounds in mulberry juice, fruits, and leaves implied that they might be potential resources for the development of functional drinks and food.

  12. 药桑椹花青素的体外抗氧化作用%Antioxidant Activity of Anthocyanins from Morus nigra L. in vitro

    Institute of Scientific and Technical Information of China (English)

    江岩; 郑力; 克热木江·吐尔逊江

    2011-01-01

    The antioxidant activity of anthocyanins from Morus nigra L.(AMNL) in vitro was determined and compared with that of anthocyanins from Morus alba L.(AMAL) and Morus alba Linn.var.tatarica.(AMALV).The antioxidant evaluation was performed by 1,1-diphenyl-2-picryl-hydrazyl(DPPH),superoxide anion,hydroxyl and alkyl free radical scavenging assays and total antioxidant capacity(TOA) assay using rutin and VC as controls.The results showed that AMNL had stronger scavenging effect on DPPH free radicals with IC50 of 9.07 mg/L than AMAL and AMALV,but weaker scavenging effect on DPPH free radicals than rutin and vitamin C.Similarly,AMNL had more powerful scavenging effect on superoxide anion free radicals with IC50 of 2.82 mg/L and hydroxyl free radicals with IC50 of 7.77 mg/L than AMAL,AMALV,rutin and VC.However,anthocyanins from all the three cultivars of mulberry had a scavenging rate of less than 50% against alkyl free radicals.The total antioxidant capacity of AMNL revealed 11.8-,2.6-and 1.3-fold enhancement when compared with AMAL,AMALV and rutin,but was lower than that of vitamin C.%研究药桑椹花青素(anthocyanins from Morus nigra L.,AMNL)的体外抗氧化作用,并与白桑椹花青素(anthocyanins from Morus alba L.,AMAL)和黑桑椹花青素(anthocyanins from Morus alba Linn.var.tatarica,AMALV)的体外抗氧化作用进行比较。以芦丁和VC为对照,测定AMNL对二苯代苦味酰肼自由基(DPPH自由基)、超氧阴离子自由基(O2.)、羟自由基(.OH)和烷基自由基的清除能力,同时测定其体外

  13. KARAKTERISASI SENYAWA FENOL DARI KAYU BATANG MORUS NIGRA

    OpenAIRE

    Ferlinahayati; Euis Holisotan Hakim; Yana Maolana Syah; Lia Dewi Juliawaty

    2013-01-01

    Tumbuhan Morus merupakan salah satu genus utama dalam famili Moraceae. Senyawa-senyawa turunan fenol merupakan kandungan utama dari genus ini, diantaranya kelompok stilben, 2-arilbenzofuran, flavonoid dan adduct Diels Alder. Penelitian ini merupakan bagian dari penelitian mengenai kajian fitokimia terhadap tumbuhan Morus. Ekstraksi dilakukan secara maserasi terhadap kayu batang Morus nigra menggunakan pelarut metanol yang dilanjutkan dengan pemisahan menggunakan berbagai teknik kromatografi s...

  14. Proximate composition and antioxidant potential of leaves from three varieties of Mulberry (Morus sp.): a comparative study.

    Science.gov (United States)

    Iqbal, Shahid; Younas, Umer; Sirajuddin; Chan, Kim Wei; Sarfraz, Raja Adil; Uddin, Kamal

    2012-01-01

    In this study, leaves of three indigenous varieties of Mulberry namely, Morus alba L., Morus nigra L. and Morus rubra L. were investigated for their antioxidant potential and their proximate composition was determined. The yields of 80% methanolic extracts ranged between 8.28-13.89%. The contents of total phenolics (TPC), total flavonoids (TFC) and ascorbic acid (AA) ranged between 16.21-24.37 mg gallic acid equivalent (GAE)/g, 26.41-31.28 mg rutin equivalent (RE)/g and 0.97-1.49 mg/g, respectively. The antioxidant activity of leaf extracts was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)) radical scavenging actity, 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid (ABTS(•+)) radical cation scavenging capacity and ferric ion reducing power and values ranged between 1.89-2.12, 6.12-9.89 and 0.56-0.97 mM Trolox equivalent/g of dried leaves, respectively. The investigated features reveal good nutritive and antioxidant attributes of all the varieties with mutually significant differences.

  15. Proximate Composition and Antioxidant Potential of Leaves from Three Varieties of Mulberry (Morus sp.: A Comparative Study

    Directory of Open Access Journals (Sweden)

    Raja Adil Sarfraz

    2012-05-01

    Full Text Available In this study, leaves of three indigenous varieties of Mulberry namely, Morus alba L., Morus nigra L. and Morus rubra L. were investigated for their antioxidant potential and their proximate composition was determined. The yields of 80% methanolic extracts ranged between 8.28–13.89%. The contents of total phenolics (TPC, total flavonoids (TFC and ascorbic acid (AA ranged between 16.21–24.37 mg gallic acid equivalent (GAE/g, 26.41–31.28 mg rutin equivalent (RE/g and 0.97–1.49 mg/g, respectively. The antioxidant activity of leaf extracts was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH radical scavenging actity, 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+ radical cation scavenging capacity and ferric ion reducing power and values ranged between 1.89–2.12, 6.12–9.89 and 0.56–0.97 mM Trolox equivalent/g of dried leaves, respectively. The investigated features reveal good nutritive and antioxidant attributes of all the varieties with mutually significant differences.

  16. Antiinflammatory properties of Morus nigra leaves.

    Science.gov (United States)

    Padilha, Marina M; Vilela, Fabiana C; Rocha, Cláudia Q; Dias, Marcelo J; Soncini, Roseli; dos Santos, Marcelo H; Alves-da-Silva, Geraldo; Giusti-Paiva, Alexandre

    2010-10-01

    The aim of the present study was to investigate antiinflammatory activity of the methylene chloride extract of Morus nigra in animal models. Carrageenan-induced paw edema as well as fibrovascular tissue growth induced by s.c. cotton pellet implantation were used to investigate the antiinflammatory activity of Morus nigra extract (MnE) in rats. A HPLC fingerprint was used for phytochemical analysis of the extracts. The MnE at test doses of 100-300 mg/kg p.o. clearly demonstrated antiinflammatory effects by reduced paw edema induced by carragenan and significantly inhibited the formation of granulomatous tissue. In addition, chemical compounds isolated from Morus nigra, including betulinic acid, β-sitosterol and germanicol, may be responsible for the antiinflammatory effect of the extract. Copyright © 2010 John Wiley & Sons, Ltd.

  17. Genetic relationships between selected Turkish mulberry genotypes (Morus spp) based on RAPD markers.

    Science.gov (United States)

    Orhan, E; Ercisli, S

    2010-11-03

    Mulberry (Morus spp, Moraceae) is an important horticultural crop in Turkey, which is one of the main world producers of mulberry fruit. We evaluated the genetic relationships among 26 mulberry genotypes selected for agronomic characteristics, using RAPD markers. A total of 367 DNA markers were generated with 34 random primers. The highest genetic similarity (0.80) was observed between Oltu58 (M. nigra) and Olur90 (M. nigra) genotypes. The genotypes Oltu3 (M. alba) and Oltu18 (M. rubra) were the most distant (0.36). We found that the RAPD technique is a useful tool to discriminate mulberry genotypes at both the intra- and interspecific level. This type of information will aid in accurate identification of useful genotypes for breeding programs.

  18. Tyrosinase inhibitory constituents from the roots of Morus nigra: a structure-activity relationship study.

    Science.gov (United States)

    Zheng, Zong-Ping; Cheng, Ka-Wing; Zhu, Qin; Wang, Xia-Chang; Lin, Zhi-Xiu; Wang, Mingfu

    2010-05-12

    The phytochemical profiles of Morus nigra roots and twigs were compared by HPLC with those of the old and young twigs of Morus alba which are known to contain oxyresveratrol and mulberroside A as major components. It was found that M. nigra root extract contains some unknown natural products with potential tyrosinase inhibitory activity. The extract (95% ethanol) of the roots of M. nigra was further investigated in this study. One new compound, 5'-geranyl-5,7,2',4'-tetrahydroxyflavone, and twenty-eight known phenolic compounds were isolated. Their structures were identified by mass spectrometry and NMR spectroscopy. Nine compounds, 5'-geranyl-5,7,2',4'-tetrahydroxyflavone, steppogenin-7-O-beta-D-glucoside, 2,4,2',4'-tetrahydroxychalcone, moracin N, kuwanon H, mulberrofuran G, morachalcone A, oxyresveratrol-3'-O-beta-D-glucopyranoside and oxyresveratrol-2-O-beta-D-glucopyranoside, showed better tyrosinase inhibitory activities than kojic acid. It was noteworthy that the IC(50) values of 2,4,2',4'-tetrahydroxychalcone and morachalcone A were 757-fold and 328-fold lower than that of kojic acid, respectively, suggesting a great potential for their development as effective natural tyrosinase inhibitors.

  19. Alba County - Rural Tourism Destination?

    Directory of Open Access Journals (Sweden)

    Claudia Olimpia Moisa

    2016-12-01

    Full Text Available The purpose of this paper is to identify the main rural touristic resources available in Alba County and also the preferred tourist destinations, highlighting the role and the importance of the rural tourism and agro-tourism in the economy of Alba County and, not least, identifying the main direction for its development and promotion. In other words, the aim of this paper is to answer the question "Is it or not Alba County a rural tourist destination?"

  20. Pityriasis alba with poliosis.

    Science.gov (United States)

    Malik, Tayyaba Gul; Khalil, Muhammad; Bhatti, Muhammad Moeen

    2014-02-01

    Pityriasis alba is a skin disease, commonly seen in children and young adults. This case presents the ocular association of this disease in a 10 years old Pakistani male. Ocular features in this case were poliosis, tilted disc, high myopia and chorioretinal degeneration. Tilted discs and high myopia can be coincidental but poliosis and decreased pigment in retinal pigment epithelium are closely related with the hypopigmentation seen in this disease.

  1. Environ: E00394 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00394 Morus alba shoot Crude drug Morus alba [TAX:3498] Moraceae (mulberry family) Morus... alba shoot Crude drugs [BR:br08305] Dicot plants: rosids Moraceae (mulberry family) E00394 Morus alba shoot ...

  2. Phenolic acids profiling and antioxidant potential of mulberry (Morus laevigata W., Morus nigra L., Morus alba L.) leaves and fruits grown in Pakistan

    Science.gov (United States)

    Mulberry trees are distributed throughout Pakistan. Besides the use of mulberry in forage and food for animals, it is also used as herbal medicine. The ojbective of this study was to determine phenolic acids profile, sugar content, and the antioxidant activity of the leaves and fruits of three mulb...

  3. Estudo fitoquímico e dos efeitos hipolipidêmico e antioxidante do extrato aquoso de amoreira preta (Morus nigra L.) em ratos submetidos à hiperlipidemia aguda /

    OpenAIRE

    Moreira, Tatianne Dias, 1978-; Zeni, Ana Lúcia Bertarello, 1968-; Universidade Regional de Blumenau. Programa de Pós-Graduação em Química.

    2015-01-01

    Orientador: Ana Lúcia Bertarello Zeni. Dissertação (Mestrado em Química) - Programa de Pós-Graduação em Química, Centro de Ciências Exatas e Naturais, Universidade Regional de Blumenau, Blumenau,

  4. Pityriasis versicolor alba.

    Science.gov (United States)

    Thoma, W; Krämer, H-J; Mayser, P

    2005-03-01

    Pityriasis versicolor alba is a hypopigmented or depigmented variant of pityriasis versicolor characterized by maculous, partly pityriasiform, scaly depigmented lesions occurring particularly in seborrhoeic areas. Long-persisting hypopigmentation after healing of the pityriasis versicolor was first described by Gudden in 1853. Hypopigmentation and depigmentation were later differentiated as an independent variant of the disease. In 1848, Eichstedt recognized the pathogen-related character of pityriasis versicolor in its hyperpigmented form. Today it is generally accepted that the disease is caused by yeasts of the genus Malassezia, of which nine species are differentiated. It is controversial whether a single species is responsible for the disease. The pathogenesis of depigmentation has not been established. A screening effect by the scale layer as well as toxic effects on pigment synthesis by fungal metabolites have been discussed. With regard to the second mechanism, the newly discovered tryptophan-derived metabolites of M. furfur might be significant. Evidence-based data concerning the therapy of pityriasis versicolor alba do not exist. According to current recommendations, pityriasis versicolor should be rapidly treated with antimycotics, followed by ultraviolet therapy to induce maturation of existent melanosomes and accelerate repigmentation. However, depigmented lesions are difficult to improve by ultraviolet therapy.

  5. Interactions of archaeal chromatin proteins Alba1 and Alba2 with nucleic acids.

    Directory of Open Access Journals (Sweden)

    Miha Črnigoj

    Full Text Available BACKGROUND: Architectural proteins have important roles in compacting and organising chromosomal DNA. There are two potential histone counterpart peptide sequences (Alba1 and Alba2 in the Aeropyrum pernix genome (APE1832.1 and APE1823. METHODOLOGY/PRINCIPAL FINDINGS: THESE TWO PEPTIDES WERE EXPRESSED AND THEIR INTERACTIONS WITH VARIOUS DNAS WERE STUDIED USING A COMBINATION OF VARIOUS EXPERIMENTAL TECHNIQUES: surface plasmon resonance, UV spectrophotometry, circular dichroism-spectropolarimetry, gel-shift assays, and isothermal titration calorimetry. CONCLUSIONS/SIGNIFICANCE: Our data indicate that there are significant differences in the properties of the Alba1 and Alba2 proteins. Both of these Alba proteins can thermally stabilise DNA polynucleotides, as seen from UV melting curves. Alba2 and equimolar mixtures of Alba1/Alba2 have greater effects on the thermal stability of poly(dA-dT.poly(dA-dT. Surface plasmon resonance sensorgrams for binding of Alba1, Alba2, and equimolar mixtures of Alba1/Alba2 to DNA oligonucleotides show different binding patterns. Circular dichroism indicates that Alba2 has a less-ordered secondary structure than Alba1. The secondary structures of the Alba proteins are not significantly influenced by DNA binding, even at high temperatures. Based on these data, we conclude that Alba1, Alba2, and equimolar mixtures of Alba1/Alba2 show different properties in their binding to various DNAs.

  6. Anticancer activity of Morus nigra leaves extract

    OpenAIRE

    Muhammad Imran Qadir; Muhammad Ali; Zubair Ibrahim

    2014-01-01

    Plants are screened for treatment of many ailments including cancer because they possess certain potential constituents which are effective for treatment. The aim of this study was to evaluate the anticancer activity of Morus nigra leaves against human cervical cancer cell line (HeLa). n-Hexane and aqueous methanolic extract of plant’s leaves were made by maceration. Anticancer activity was estimated by methyl-thiazolyl-tetrazolium (MTT) assay and percentage inhibition of cells was calculated...

  7. KARAKTERISASI SENYAWA FENOL DARI KAYU BATANG MORUS NIGRA

    Directory of Open Access Journals (Sweden)

    Ferlinahayati

    2013-05-01

    Full Text Available Tumbuhan Morus merupakan salah satu genus utama dalam famili Moraceae. Senyawa-senyawa turunan fenol merupakan kandungan utama dari genus ini, diantaranya kelompok stilben, 2-arilbenzofuran, flavonoid dan adduct Diels Alder. Penelitian ini merupakan bagian dari penelitian mengenai kajian fitokimia terhadap tumbuhan Morus. Ekstraksi dilakukan secara maserasi terhadap kayu batang Morus nigra menggunakan pelarut metanol yang dilanjutkan dengan pemisahan menggunakan berbagai teknik kromatografi sehingga diperoleh senyawa murni. Berdasarkan data-data spektroskopi yang meliputi spektrum UV, IR dan NMR maka senyawa murni hasil isolasi tersebut merupakan senyawa fenol yaitu b-resorsilaldehid

  8. Toxic effects of cadmium on Morus alba L. and Bombyx moril L.

    NARCIS (Netherlands)

    Wang, K.R.; Gong, H.; Wang, Y.; Zee, van der S.E.A.T.M.

    2004-01-01

    A 3-year micro-plot experiment of mulberry cultivation with Cd-polluted soil and silkworm breeding experiments by feeding with exogenous or endogenous ¿Cd-polluted mulberry leaves were conducted to evaluate the toxic effects of Cd on mulberry and silkworms. There was no apparent harmful effect on mu

  9. Consumo de morera (Morus alba y sorgo negro forrajero (Sorghum almum en ganado Jersey

    Directory of Open Access Journals (Sweden)

    Carlos Boschini

    2000-01-01

    Full Text Available Se cosecharon ramas de morera de 84 días y forraje de sorgo negro forrajero de 77 días de rebrote. El forraje fue suministrado a seis toretes Jersey con un peso vivo promedio de 337 (±24,27 kg, distribuidos en un diseño de cuadrado latino 3 x 3, repetido. Los tratamientos experimentales fueron: a.- suministro único de morera, b.- 50% de morera y 50% de sorgo negro forrajero en base verde tal como fue cosechada y c.- suministro único de sorgo negro forrajero. Ambos forrajes se ofrecieron a los animales en forma picada (hojas y tallos. La mezcla de morera y sorgo se realizó en partes iguales en base al peso fresco. Cada animal recibió 50 kg/día de forraje verde. La relación promedio de hoja:tallo en la morera fue de 1,3 (±0,39 y de 0,84 (±0,26 en el sorgo negro. Se recolectaron muestras del material ofrecido y rechazado para el análisis de materia seca, proteína cruda, fibra neutro detergente y cenizas totales. No se observaron diferencias (p>0,05 en el consumo de materia fresca y de materia seca (kg/animal/día entre los tratamientos. La ingesta de materia seca por cada 100 kg de peso vivo de los animales fue de 1,94% para la dieta de morera, 1,82% para la mezcla morera/sorgo y 1,67% para la dieta de sorgo negro, lo cual fue inferior al 2% esperado

  10. Comportamiento productivo de reproductoras porcinas alimentados con follaje fresco de Morus alba. IIndicadores reproductivos

    Directory of Open Access Journals (Sweden)

    Contino Yuván; Ojeda Félix ; Herrera Rafael ; Altunaga Nancy; Pérez Guadalupe.

    2008-08-01

    Full Text Available El objetivo de esta investigación consistió en evaluar el consumo, larespuesta productiva y reproductiva de cerdas mestizas al emplear elfollaje de morera fresca (hojas y tallos tiernos como sustituto parcial del concentrado comercial y su repercusión en la salud de las crías. Las investigaciones se efectuaron en el Módulo de Investigación-Producción Porcina de la EEPF ”Indio Hatuey”. Se realizó una experiencia con 10 reproductoras en diferentes estados reproductivos alimentadas a voluntad con morera fresca, para determinar el consumo; y otra evaluación con reproductoras en el último tercio de la gestación que consumían morera, a voluntad para comparar sus indicadores productivos y de salud de las crías con respecto a un grupo control que no consumió el follaje fresco. Sedeterminaron los siguientes indicadores: Cantidad de cerdos nacidos, crías vivas y muertas a las 48 horas, peso al nacimiento y a los 10 días posparto,peso total de la camada al nacimiento y a los 10 días post-parto, peso al destete, porcentaje de viabilidad y de mortalidad a las 48 horas, número de crías con diarreas a las 48 horas. Para el procesamiento se empleó el paquete estadístico SPSS versión 10.1. No hubo diferencias en los índices de consumo entre las diferentes categorías reproductivas. Se obtuvo un incremento en la cantidad de crías nacidas, crías vivas a las 48 horas, en elpeso promedio de la camada, del peso al destete así como en el porcentaje de viabilidad con una disminución en las diarreas de las crías. Los resultados demuestran la factibilidad económica de esta alternativa alimentaria

  11. Degradability of mulberry (Morus alba and rice bran in the rumen of sheep fed different diets

    Directory of Open Access Journals (Sweden)

    Dwi Yulistiani

    2008-12-01

    Full Text Available The experiment was conducted to investigate degradation of dry matter (DM and protein of mulberry and rice bran when incubated in nylon bag in the rumen at different incubation times and different rumen environments of rumen-cannulated adult sheep. Three different rumen conditions were created by feeding the three rumen-cannulated sheep with urea-treated rice straw as basal diet and offered with three supplemental treatment diets on different source of energy and nitrogen. Mulberry, urea and rice bran were used as source of fermentable energy and protein. Treatments consisted of control diet mulberry and molasses (T0; 50% mulberry was replaced by rice bran and urea (T1; and 100% of mulberry was replaced with rice bran and urea (T2. The diets were formulated in iso protein and iso energy. Supplemental diets were offered at 1.2% BW. The study was conducted in three periods. For each period, the sheep was offered with one of three supplemental treatment diets. The nylon bags each, contains sample of either mulberrry or rice bran were incubated in the rumen of sheep at different incubation times in reverse order (48, 24, 12, 9, 6, and 3h. Degradation characteristic data were obtained by analyzing degradability data with the equation of p = a+b(1-e-ct using Neway computer package. Data of degradation characteristic, degradability of DM and CP, DMI, rumen NH3-N and pH were subjected to analysis of variance (ANOVA using a SAS software package. The results showed that the dry matter intake (DMI and rumen pH were not significantly different between diets. The rumen ammonia concentration of T1 and T2 was significantly higher than that of T0. However, the rumen ammonia concentration was higher than that of critical value for rumen microbial synthesis (>5mg/100ml. The rate (c value of DM degradability of mulberry and rice bran was affected by diet treaments, where T2 diet resulted in lower c of mulberry and rice bran. Only CP degradability of mulberry on the other hand was affected by diet treatments. But, both CP mulberry and rice bran had high degradability (>80% after 24 hs incubation in all diet treatments. It is concluded that the three diets of this study were capable of creating the optimum condition for rumen fermentation. Supplementation of mulberry or urea-rice bran mixture had similar effect on protein degradability of rice bran. On the other hand, the rate of protein degradability of mulberry was reduced when it was incubated in the rumen of sheep fed urea rice bran mix supplement.

  12. In vitro propagation of Morus alba L. in semisolid culture medium.

    Directory of Open Access Journals (Sweden)

    Enrique Salas Barbosa

    2005-04-01

    Full Text Available Apical buds as explants were used with the objective to propagate in vitro mulberry plants in semisolid MS culture medium suplemented with 6-BAP and KIN in their establishment and, with different combinations of 6-BAP with ANA in the multiplication phase. In vitro plants were evaluated during the acclimatization phase. It is necessary to supplement the basal MS culture media with 0.5 mg.l-1 of 6-BAP to induce the sprouting and, 0.5 mg.l-1 of 6-BAP and 0.5 mg.l-1 of ANA to multiply the mulberry by nodal segments. In the acclimatization phase a 95% of survival, 30.2 cm of height, 9.8 leaves and 2.02 g.plant-1 of dry mass was observed. In vitro propagation of mulberry was achieved as an alternative for plants production. Key words: acclimatization, apical buds, establishment, explant, shooting

  13. Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves.

    Science.gov (United States)

    Yuan, Qingxia; Xie, Yufeng; Wang, Wei; Yan, Yuhua; Ye, Hong; Jabbar, Saqib; Zeng, Xiaoxiong

    2015-09-05

    Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry leaves (MLP) were investigated in the present study. The optimal extraction conditions with an extraction yield of 10.0 ± 0.5% for MLP were determined as follows: extraction temperature 92 °C, extraction time 3.5h and ratio (v/w, mL/g) of extraction solvent (water) to raw material 34. Two purified fractions, MLP-3a and MLP-3b with molecular weights of 80.99 and 3.64 kDa, respectively, were obtained from crude MLP by chromatography of DEAE-Cellulose 52 and Sephadex G-100. Fourier transform-infrared spectroscopy revealed that crude MLP, MLP-3a and MLP-3b were acidic polysaccharides. Furthermore, crude MLP and MLP-3a had more complicated monosaccharide compositions, while MLP-3b had a relatively higher content of uronic acid. Crude MLP, MLP-3a and MLP-3b exhibited potent Fe(2+) chelating power and scavenging activities on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide and 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid) radicals. The results suggested that MLP could be explored as natural antioxidant.

  14. Ecologia da polinização da amoreira-preta (Rubus sp (Rosaceae em Timbó-SC, Brasil

    Directory of Open Access Journals (Sweden)

    Leônidas João de Mello Junior

    2011-09-01

    Full Text Available O presente trabalho verificou experimentalmente a ecologia da polinização da amoreira-preta (Rubus sp.. Objetivou-se confirmar a síndrome de polinização de Rubus sp., por meio de análise de seu sistema reprodutivo, quantificação da produção diária de néctar, levantamento da entomofauna que visita as flores da amoreira na área de estudo e análise do seu comportamento polinizador. Os experimentos foram realizados em uma área de cultivo de amoreira-preta, no mês de dezembro de 2005, município de Timbó (SC. Constatou-se que a maior taxa de frutificação (48,3 % ± 3,2 ocorreu via polinização livre, onde os insetos não foram excluídos. Foi verificada a ocorrência de autopolinização, porém com taxa inferior de frutificação (12,2% ± 4,9. A anemofilia não foi constatada na espécie. A avaliação da produção de néctar em Rubus sp. resultou em valores compatíveis com a síndrome de melitofilia. Os visitantes florais coletados e observados sobre as flores de Rubus sp. foram predominantemente abelhas da ordem Hymenoptera (97%, que iniciam, em menor número, a atividade de forrageamento às 8h, com pico de atividade às 12h e declinando até às 16h. As coletas resultaram numa amostragem de 1.360 abelhas, divididas em quatro famílias e 13 espécies. A família com maior riqueza de espécies (N = 7 foi Halictidae e a mais abundante foi Apidae, com 1.288 indivíduos. Em Apidae, houve o predomínio da espécie exótica Apis mellifera, cuja representação na amostra foi de 1.246 indivíduos. Os dados da abundância e as observações naturalísticas do seu comportamento da flor apontaram A. mellifera como o principal polinizador de Rubus sp., mas confirmam que as demais espécies de abelhas também participam na polinização de Rubus sp.

  15. Produção de amoreira-preta sob diferentes sistemas de condução

    Directory of Open Access Journals (Sweden)

    Letícia Vanni Ferreira

    2016-03-01

    Full Text Available RESUMO: O objetivo do trabalho foi avaliar o efeito da produção e qualidade dos frutos de cultivares de amoreiras-pretas conduzidas em diferentes sistemas de condução. O delineamento experimental adotado foi o de blocos casualizados em parcelas subdivididas, em um fatorial 3x3. Utilizaram-se as cultivares 'Tupy', 'Guarani' e 'Xavante' e os sistemas de condução: sem tutor, espaldeira em "T" e espaldeira em fios paralelos. Avaliou-se a produção de frutas, o número médio de frutas, a massa média de frutas por planta nas safras de 2009/2010, 2010/2011 e 2011/2012. Nas duas últimas safras, também foi avaliado o conteúdo de sólidos solúveis. Os sistemas de condução influenciaram no comportamento produtivo das amoreiras-pretas, sendo que o sistema em espaldeira em "T" proporcionou maior produção e número de frutas, no entanto, a maior massa média de frutas foi obtida nas plantas conduzidas sem tutor. As plantas conduzidas em espaldeira em "T" e espaldeira apresentaram maior produção (1357,4 e 1217,8g planta-1, respectivamente e número de frutas (341,7 e 320, respectivamente. Dentre as cultivares, a produção diferiu entre elas, destacando-se a 'Tupy' e 'Guarani' com 2098g e 2094g planta-1, respectivamente. Entretanto, o maior número de frutas por planta foi superior nas plantas de 'Guarani', sendo inversamente proporcional à massa média de frutas para esta cultivar. A cultivar 'Tupy' apresenta maior produção em relação às demais cultivares avaliadas. O número médio de frutas por planta foi superior nas plantas de 'Guarani'. Nas condições do presente trabalho conclui-se que o desempenho produtivo de amoreira-preta depende de cada cultivar e também do sistema de condução adotado.

  16. Brazilian Morus nigra Attenuated Hyperglycemia, Dyslipidemia, and Prooxidant Status in Alloxan-Induced Diabetic Rats

    National Research Council Canada - National Science Library

    Ivanildo I. da S. Júnior; Humberto de Moura Barbosa; Débora C. R. Carvalho; Ruideglan de Alencar Barros; Flávia Peixoto Albuquerque; Dionísio Henrique Amaral da Silva; Grasielly R. Souza; Nathália A. C. Souza; Larissa A. Rolim; Flaviane M. M. Silva; Glória I. B. P. Duarte; Jackson R. G. da S. Almeida; Flávio Monteiro de Oliveira Júnior; Dayane A. Gomes; Eduardo C. Lira

    2017-01-01

      Morus nigra has been used popularly for several proposes, including diabetic. In an attempt to support medicinal value, the acute hypoglycemic, hypolipidemic, and antioxidant effects of the ethanolic extract of Morus nigra...

  17. Adubação nitrogenada e características agronômicas em amoreira-preta

    Directory of Open Access Journals (Sweden)

    Ivan dos Santos Pereira

    2013-04-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência de doses crescentes de nitrogênio sobre o crescimento vegetativo, a produção e os níveis foliares de nutrientes da amoreira-preta (Rubus spp.. O experimento foi realizado com as cultivares Tupy e Xavante, na região sul do Rio Grande do Sul, de 2008 a 2012. Os tratamentos consistiram da aplicação de cinco doses de N (0,0, 7,5, 15,0, 22,5 e 30,0 g por planta, aplicados na adubação de manutenção, após a implantação da lavoura. Utilizou-se o delineamento de blocos ao acaso, com quatro repetições. Foram avaliadas as variáveis: densidade de hastes, massa de matéria seca de poda, índice de clorofila, produção por planta, número de frutos e composição nutricional das folhas (N, K, Ca, Mg e S. A adubação nitrogenada influenciou significativamente o crescimento vegetativo, a produção e a composição nutricional das folhas. As cultivares apresentaram resposta distinta à aplicação de N, em que 'Tupy' foi mais exigente que 'Xavante'. As doses de N atualmente recomendadas para a cultura da amoreira-preta são satisfatórias para a máxima produção de 'Xavante', mas insuficientes para 'Tupy'.

  18. Teor de compostos fenólicos e atividade antioxidante em diferentes genótipos de amoreira-preta (Rubus sp.)

    OpenAIRE

    Márcia Vizzotto; Maria do Carmo Bassols Raseira; Marina Couto Pereira; Mariana da Rosa Fetter

    2012-01-01

    Tendo em vista que alguns compostos fenólicos encontrados em amora-preta são benéficos para a saúde humana, este estudo foi conduzido com o propósito de quantificar os teores de compostos fenólicos totais e a atividade antioxidante em diferentes genótipos produzidos por 10 seleções e 4 cultivares de amoreira-preta com ou sem espinhos. Quanto aos resultados, pode-se observar que, dentre as seleções e cultivares de amoreira-preta com espinho, a seleção S16/96 apresentou o maior teor de composto...

  19. Alba Patera Collapse Pits

    Science.gov (United States)

    2004-01-01

    [figure removed for brevity, see original site] We will be looking at collapse pits for the next two weeks. Collapse pits on Mars are formed in several ways. In volcanic areas, channelized lava flows can form roofs which insulate the flowing lava. These features are termed lava tubes on Earth and are common features in basaltic flows. After the lava has drained, parts of the roof of the tube will collapse under its own weight. These collapse pits will only be as deep as the bottom of the original lava tube. Another type of collapse feature associated with volcanic areas arises when very large eruptions completely evacuate the magma chamber beneath the volcano. The weight of the volcano will cause the entire edifice to subside into the void space below it. Structural features including fractures and graben will form during the subsidence. Many times collapse pits will form within the graben. In addition to volcanic collapse pits, Mars has many collapse pits formed when volatiles (such as subsurface ice) are released from the surface layers. As the volatiles leave, the weight of the surrounding rock causes collapse pits to form. This image of the Alba Patera region has both lava tube collapse pits (running generally east/west) and subsidence related collapse within structural grabens. Image information: IR instrument. Latitude 26.9, Longitude 256.5 East (103.5 West). 100 meter/pixel resolution. Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time. NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science

  20. Exigência térmica e produtividade da amoreira-preta em função das épocas de poda

    Directory of Open Access Journals (Sweden)

    Daniela Mota Segantini

    2014-09-01

    Full Text Available O período de colheita de amora-preta ocorre de novembro a fevereiro nos estados do Sul do Brasil, onde se concentra a maior área de cultivo do País. Sabendo-se que frutas ofertadas fora do pico da safra possuem preços mais vantajosos, objetivou-se escalonar a colheita da amoreira-preta através da realização da poda hibernal em diferentes épocas, levando-se em conta o número de horas de frio e os grausdia acumulados. O presente trabalho foi conduzido na Fazenda Experimental São Manuel, no município de São Manuel-SP. Foram utilizadas plantas de amoreira-preta "Tupy", de 3 anos de idade, conduzidas em 4 hastes principais, em espaldeira em T, com 1,2 metro de altura. O delineamento experimental utilizado foi o esquema fatorial 2x5, constando de 2 sistemas de cultivo (não irrigado e irrigado e de 5 tratamentos (épocas de poda: 20-05, 26-06, 24-07, 23-08 e 26-09, com repetições em blocos. A irrigação não alterou a duração do ciclo da amoreira-preta, mas proporcionou aumento na produtividade, independentemente das épocas de poda. Quanto maior o número de horas de frio acumulado, menor o ciclo e menor a necessidade em graus-dia da amoreira-preta 'Tupy'. A poda hibernal realizada em 20-05 proporcionou um pico de colheita de amora-preta em meados de setembro, em São Manuel - SP.

  1. Teor de compostos fenólicos e atividade antioxidante em diferentes genótipos de amoreira-preta (Rubus sp.

    Directory of Open Access Journals (Sweden)

    Márcia Vizzotto

    2012-09-01

    Full Text Available Tendo em vista que alguns compostos fenólicos encontrados em amora-preta são benéficos para a saúde humana, este estudo foi conduzido com o propósito de quantificar os teores de compostos fenólicos totais e a atividade antioxidante em diferentes genótipos produzidos por 10 seleções e 4 cultivares de amoreira-preta com ou sem espinhos. Quanto aos resultados, pode-se observar que, dentre as seleções e cultivares de amoreira-preta com espinho, a seleção S16/96 apresentou o maior teor de compostos fenólicos e atividade antioxidante. Dentre as seleções sem espinho, a S17/01 e a S02/96 apresentaram os maiores valores, não diferindo da S12/01 para a atividade antioxidante. Não houve diferença estatística para os teores de compostos fenólicos totais e atividade antioxidante, quando comparadas entre si as seleções e as cultivares de amoreira-preta com espinhos. A correlação entre o teor de compostos fenólicos totais e a atividade antioxidante nas amoras-pretas estudadas é baixa, indicando presença de outros fitoquímicos e/ou vitaminas que podem influenciar o poder antioxidante.

  2. Anticancer activity of Morus nigra leaves extract

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    Muhammad Imran Qadir

    2014-12-01

    Full Text Available Plants are screened for treatment of many ailments including cancer because they possess certain potential constituents which are effective for treatment. The aim of this study was to evaluate the anticancer activity of Morus nigra leaves against human cervical cancer cell line (HeLa. n-Hexane and aqueous methanolic extract of plant’s leaves were made by maceration. Anticancer activity was estimated by methyl-thiazolyl-tetrazolium (MTT assay and percentage inhibition of cells was calculated. Results of MTT showed that 100 µg/ml aqueous methanol extract of M. nigra inhibited 89.5-31.99% of HeLa cell line. It was concluded that M. nigra possess anticancer activity.

  3. Three new compounds from Morus nigra L.

    Science.gov (United States)

    Wang, Lei; Yang, Yan; Liu, Chao; Chen, Ruo-Yun

    2010-06-01

    A new 2-arylbenzofuran derivative, mornigrol D (1), along with two new flavones, mornigrol G (2) and mornigrol H (3), and six known compounds, norartocarpetin (4), dihydrokaempferol (5), albanin A (6), albanin E (7), moracin M (8), and albafuran C (9), were isolated from the barks of Morus nigra. Their structures were elucidated by spectroscopic analysis. Compounds 1 and 9 showed antioxidative activities in vitro with inhibition ratios of 98 and 99% at the concentration of 10(-4) mol/l, and of 74 and 75% at the concentration of 10(-5) mol/l. In addition, compounds 1 and 4 showed potent anti-inflammatory activities (inhibition of release of beta-glucuronidase from rat polymorphonuclear leucocytes induced by platelet activating factor) with inhibitory ratios of 65.9% (P < 0.01) and 67.7% (P < 0.01) at a concentration of 10(-5) mol/l.

  4. Extraction and antioxidant activity of flavonoids of Morus nigra.

    Science.gov (United States)

    Feng, Rui-Zhang; Wang, Qin; Tong, Wen-Zhi; Xiong, Juan; Wei, Qin; Zhou, Wan-Hai; Yin, Zhong-Qiong; Yin, Xiao-Ya; Wang, Li-Ying; Chen, Ya-Qin; Lai, Yong-Hong; Huang, Hong-Yan; Luo, Qiao-Li; Wang, Lu; Jia, Ren-Yong; Song, Xu; Zou, Yuan-Feng; Li, Li-Xia

    2015-01-01

    Morus nigra has a long history of medicinal use in Chinese medicine, but the study on it is limited, the flavonoids are one of the main biological active substances. In this study, the Morus nigra flavonoids were extracted by ultrasonic and antioxidant activities both in vitro and in vivo were measured. The results showed that hydroxyl radicals clearance rate and superoxide radical anion clearance rate in vitro increased with the concentration of the total flavonoids in the range of 0-1.05 mg/mL and the maximum clearance rate was 80.33% and 87.69%, respectively. After mice were treated with flavonoids, the content of malonaldehyde (MDA) in serum and liver decreased; the activities of superoxide dismutase (SOD) in serum and liver, catalase (CAT) in liver and glutathione peroxidase (GSH-PX) in blood and liver increased; Langhans cells increased in spleen. These results revealed that the Morus nigra flavonoids possessed strong antioxidant activity.

  5. Multiplicação in vitro de amoreira-preta cultivar Brazos In vitro multiplication of blackberry cv. Brazos

    Directory of Open Access Journals (Sweden)

    Fabiola Villa

    2006-04-01

    Full Text Available A micropropagação da amoreira-preta pode gerar plantas livres de vírus e em curto espaço de tempo. Com o objetivo de aprimorar técnicas de micropropagação de amoreira-preta cultivar Brazos (Rubus idaeus L., segmentos nodais, oriundos de plântulas preestabelecidas in vitro foram excisados e inoculados em meio WPM (0, 50, 100, 150 e 200%, suplementado com diferentes concentrações de BAP (0; 0,5; 1,0; 2,0 e 4,0 mg L-1. Após a inoculação, os explantes foram transferidos para sala de crescimento a 27±1ºC, irradiância de 35 mmol m² s¹ e fotoperíodo de 16 horas, onde permaneceram por 60 dias. O delineamento experimental utilizado foi inteiramente casualizado, utilizando-se de quatro repetições com quatro explantes cada. Maior número de brotos foi proporcionado com 1,0 mg L-1 de BAP associado a 100% de meio WPM e maior comprimento médio dos brotos após 60 dias foi verificado em 1,0 mg L-1 de BAP associado a 200% de meio WPM. Maior peso de matéria seca da parte aérea foi obtido em meio WPM 200% acrescido de 0,5 mg L-1 de BAP.With the objective of multiplying blackberry cv. Brazos, nodal segments, coming from in vitro plants previously selected, were excised and inoculated in WPM culture medium (0, 50, 100, 150 and 200%, supplemented with different concentrations of BAP (0; 0,5; 1,0; 2,0 and 4,0 mg L-1. After inoculation, the explants were transferred to culture room, at 27±1ºC temperature, 35 mmol m² s¹ ofirradiance and photoperiod of 16 hours, for 60 days. The experimental was a design randomized complete block, with four replications and four explants each. Greater number of sprouts was provided with 1,0 mg L-1 of BAP associated with 100% WPM culture medium and larger sprouts length average after 60 days were verified in 1,0 mg L-1 of BAP associated with 200% WPM culture medium. Higher dry matter weight of the aerial part was obtained in 200% WPM culture medium added with 0,5 mg L-1 of BAP.

  6. Propagação de amoreira-preta utilizando estacas lenhosas Propagation of blackberry using of woody cutting

    Directory of Open Access Journals (Sweden)

    Fabíola Villa

    2003-08-01

    Full Text Available A amoreira-preta (Rubus sp é uma espécie de clima temperado que, por ocasião da poda hibernal, realizada durante o período de dormência, produz grande quantidade de material vegetal que pode ser utilizado na propagação. Dessa forma, objetivou-se verificar o potencial de enraizamento de estacas lenhosas de duas cultivares de amoreira-preta tratadas com ácido indolbutírico (AIB. De ramos com um ano de idade coletados de plantas-matrizes das cultivares Brazos e Guarani, foram retiradas estacas lenhosas, sem folhas, com aproximadamente 15 cm e tratadas com solução de AIB, nas concentrações de 0, 1000, 2000 e 3000 mg.L-1, por 15 segundos. Posteriormente, as estacas foram plantadas em sacos de polietileno contendo mistura de areia e terra (2:1 v/v como substrato e mantidas em casa-de-vegetação, sob nebulização intermitente durante 90 dias.As cultivares apresentaram diferentes potenciais de enraizamento e desenvolvimento da parte aérea. Maiores percentuais de estacas enraizadas e brotadas, número de folhas e de brotos e peso da matéria seca das brotações foram obtidos em estacas de ‘Brazos' não-tratadas e em estacas de ‘Guarani' com aplicação de 2000 mg.L-1 de AIB.During the winter pruning, in the dormancy period, there are surpluses of great amount of vegetable material that can be used in the propagation. It was aimed at verifying the hardwood cuttings rooting potential of two blackberry cultivar treated with indolebutyric acid (IBA. Hardwood cuttings without leaves, with approximately 15 cm were collected from branches with one year old of ‘Brazos' and ‘Guarani' mother-plant and treated with solution of IBA at of 0, 1000, 2000 and 3000 mg.L-1 concentrations for 15 seconds immersion. Later the cuttings were planted in polyethylene bags containing sand and soil mixture (2:1 v/v as substrate and maintained in greenhouse under intermittent mist for 90 days. The cultivars presented them different rooting potential and

  7. Phytochemical Content of Some Black (Morus nigra L. and Purple (Morus rubra L. Mulberry Genotypes

    Directory of Open Access Journals (Sweden)

    Murat Tosun

    2010-01-01

    Full Text Available Bright black (Morus nigra and purple mulberry (Morus rubra are particularly desirable fruits in Turkey. More recently, the interest in these bright black and purple mulberry fruits has also increased because of the popularization of healthy properties of these fruits. The study was carried out in 2008 aiming to determine the antioxidant activity (ferric reducing ability of plasma, FRAP, total phenolic, total anthocyanin, mineral, soluble solid, vitamin C, and total acid content of four black and four purple mulberry genotypes grown in Turkey. The results show that black mulberry genotypes have a higher bioactive content than purple mulberry genotypes. The average total phenolic content and total anthocyanins of black mulberry genotypes were 2149 μg of gallic acid equivalent (GAE per g and 719 μg of cyanidin 3-glucoside equivalent (Cy 3-glu per g of fresh mass. In purple mulberry, these values were for GAE 1690 μg/g and for Cy 3-glu 109 μg/g on fresh mass basis. The average antioxidant activity of black mulberry genotypes was also found to be higher than that of the purple ones according to FRAP assay (Trolox equivalent (TE per fresh mass of black and purple mulberries was 13.35 and 6.87 μmol/g, respectively.

  8. PROMOTIONAL STRATEGY OF THE ALBA IULIA FORTRESS

    OpenAIRE

    Andreea Muntean; Lucian Marina

    2008-01-01

    The specific character of The Fortress of Alba Iulia results from its value as anelement of historical and cultural patrimony. This value must be kept and brought into prominence,and for this it must be promoted with the help of cultural tourism. This paper presents some issuesregarding the promotional strategy of the Alba Iulia Fortress.

  9. Isoprenylated flavonoids and adipogenesis-promoting constituents from Morus nigra.

    Science.gov (United States)

    Hu, Xiao; Wu, Jin-Wei; Zhang, Xiao-Dong; Zhao, Qin-Shi; Huang, Jian-Ming; Wang, He-Yao; Hou, Ai-Jun

    2011-04-25

    Ten new isoprenylated flavonoids, nigrasins A-J (1-10), and three known compounds were isolated from the twigs of Morus nigra. Compounds 8 and 9 promoted adipogenesis, characterized by increased lipid droplet and triglyceride content in 3T3L1 cells, and induced up-regulation of the expression of adipocyte-specific genes, aP2 and GLUT4.

  10. Three new compounds from the barks of Morus nigra.

    Science.gov (United States)

    Wang, Lei; Cui, Xi-Qiang; Gong, Ting; Yan, Ren-Yi; Tan, Yong-Xia; Chen, Ruo-Yun

    2008-01-01

    Three new compounds including two flavonoids and a new 2-phenylbenzofuran, named morunigrols A-C (1-3), together with three known compounds albafuran A (4), albafuran B (5), and mulberrofuran L (6), have been isolated from the barks of Morus nigra. Their structures have been elucidated by spectroscopic methods.

  11. Composição química, compostos bioativos e dissimilaridade genética entre cultivares de amoreira (Rubus spp. cultivadas no Sul de Minas Gerais

    Directory of Open Access Journals (Sweden)

    Mayara Neves Santos Guedes

    2014-03-01

    Full Text Available Frutos das cultivares Arapaho, Brazos, Caingangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy e Xavante, e uma espécie de amoreira-vermelha (Rubus rosifolius Smith foram avaliados quanto à composição química. Avaliou-se também a variação genética entre as cultivares de amoreira-preta e a espécie de amoreira-vermelha. Os resultados demonstraram variações na composição química dos frutos estudados. A amora- vermelha apresentou menor teor de umidade e maiores valores para os componentes cinzas, açúcares totais, açúcares redutores, açúcares näo redutores, pectina total, pectina solúvel, fenóis, flavonoides, licopeno, β-caroteno e vitamina A. Os teores de umidade e antocianinas, a porcentagem de solubilização e a atividade antioxidante foram maiores nos frutos da cultivar Ébano. Verificou-se que os frutos da amoreira-vermelha e da cultivar Ébano apresentaram o maior grau de divergência genética para as variáveis analisadas. Isto indica a possibilidade de uso das mesmas em programas de melhoramento que visem à melhoria da composição química.

  12. Antioxidant activity of three extracts of Morus nigra.

    Science.gov (United States)

    Naderi, Gholam Ali; Asgary, Seddigheh; Sarraf-Zadegan, Nizal; Oroojy, Heshmatallah; Afshin-Nia, Farsad

    2004-05-01

    The antioxidant effect of three different extracts of Morus nigra fruit (fruit juice, hydroalcoholic and polyphenolic) on haemoglobin glycosylation, peroxidative damage to human erythrocytes, liver hepatooytes of rats and human low-density lipoprotein (LDL) were studied. The results show that all three extracts inhibited haemoglobin glycosylation induced by glucose to differing degrees. The haemolysis of human erythrocytes induced by hydrogen peroxide was also inhibited. The production of malondialdehyde (MDA) during peroxidative damage to plasma membranes of isolated rat hepatocytes induced by tert-butyl hydroperoxide (tBH) was also inhibited. Inhibition of lipid peroxidation of LDL induced by copper (II) ion was achieved during the study. The results suggest that Morus nigra fruit has a protective action against peroxidative damage to biomembranes and biomolecules. Copyright 2004 John Wiley & Sons, Ltd.

  13. [Studies on chemical constituents from bark of Morus nigra].

    Science.gov (United States)

    Wang, Lei; Wang, Hong-Qing; Chen, Ruo-Yun

    2007-12-01

    To investigate the chemical constituents in the barks of Morus nigra. The nine compounds were isolated and purified by column chromatography over silica gel, LH -20 and preparative HPLC and their structures were identified by various spectroscopic methods. The nine compounds were isolated and identified as olcancolic acid , apigenin, cyclocommunol, morusin, cyclomorusin, kuwanon C, daucosterol, ursolic acid, 63-sitosterol. Olcancolic acid, apingenin, cyclocommunol, morusin, cyclomorusin, kuwanon C, daucosterol were firstly isolated from the M. nigra.

  14. Analysis of Genetic Relationship of 24 Mulberry (Morus L.) Variety Resources Based on SRAP Molecular Markers%24份桑树品种资源基于SRAP分子标记的亲缘关系分析

    Institute of Scientific and Technical Information of China (English)

    胡德昌; 张萍; 王照红; 陈传杰

    2015-01-01

    应用SRAP分子标记引物对24份桑树品种资源的基因组DNA进行PCR,检测其遗传多样性,分析品种间的亲缘关系.15对SRAP引物扩增产生126条谱带,平均每对引物产生8.4条谱带,其中多态性条带共99条,多态性比率为78.57%,说明24个桑树品种间具有丰富的遗传多样性.基于品种间遗传相似系数的UPGMA聚类图和基于品种的SRAP标记类型的主成分分析中,蒙桑种(Morus mongolica Schneid)的品种吉蒙桑、白桑种(Morus albaL.)的品种垂枝桑和山桑种(Morus bombycis Koidz.)的品种昌农山桑单独聚类,其他来自白桑种、广东桑种(Morus atropurpurea Roxb.)、鲁桑种(Morus multicaulis Perr.)的栽培品种分为3组;白桑种种内各品种间的遗传变异较小,而广东桑种、鲁桑种种内各品种间的遗传变异相对较大;广东桑3个品种间的遗传背景差异较大;鲁桑栽培品种农桑12号与其父本桐乡青的遗传相似度高,而与母本北区一号的差异显著,并且鲁桑种内各品种间未能完全聚类,品种间的亲缘关系与地理分布位置的距离相关.筛选的15对SRAP引物能够有效地检测供试桑树品种的遗传多样性,揭示品种间的亲缘关系.

  15. SUCO INTEGRAL DE AMORA (MORUS NIGRA L. OBTIDO ATRAVÉS DE PRENSAGEM E DESPOLPAMENTO: RENDIMENTO E CARACTERIZAÇÃO FÍSICO-QUÍMICA

    Directory of Open Access Journals (Sweden)

    Suzana Magda PIMENTA

    2012-04-01

    Full Text Available A amora (Morus nigra L. é uma fruta com grande potencial de aproveitamento tecnológico, podendo ser usada para fabricação de xaropes, sorvetes, doces, iogurtes, bebidas, geleias, etc. Da amoreira, apenas a folha tem sido explorada comercialmente para alimentação do bicho-da-seda. Essa planta é cultivada em diversos ambientes agrícolas, entre eles, pequenas propriedades rurais e assentamentos. Por desconhecimento dos produtores, perde-se grande parte de sua produção. Assim, o objetivo deste trabalho foi avaliar o rendimento de extração e as características físico-químicas do suco integral de amoras maduras, verdes e mistura de ambas utilizando prensa hidráulica e despolpadora vertical. A análise estatística dos resultados foi feita através do cálculo da média e do desvio padrão. Foram colhidos cerca de 173kg de amora, sendo 49,31% de maduras e 50,68% de verdes. A prensa hidráulica proporcionou maior rendimento de extração de suco integral (80,50 a 81,6% em relação à despolpadora (71,43 a 77,50%. O suco integral obtido nos dois equipamentos apresentou valores de sólidos solúveis de 7,45 a 12,11°Brix, acidez titulável de 0,45 a 1,23g.100mL-1, Ratio de 6,03 a 26,84, pH de 3,69 a 4,35, açúcares redutores totais de 4,05 a 9,96g.100mL-1, açúcares redutores de 3,95 a 9,15g.100mL-1, sacarose de 0,00 a 0,04g.100mL-1 e turbidez de 221,25 a >4000NTU. Os sucos integrais obtidos em ambos os equipamentos apresentaram características físico-químicas similares exceto para turbidez. Concluiu-se que a prensa foi o melhor equipamento de extração, devido ao maior rendimento e qualidade do suco integral em termos de turbidez.

  16. Antioxidant Effect of Lippia alba (Miller) N. E. Brown

    OpenAIRE

    Chies, Claire; Branco, Cátia; Scola, Gustavo; Agostini, Fabiana; Gower,Adriana; Salvador, Mirian

    2013-01-01

    Lippia alba is a shrub found in all regions of Brazil and other countries in South and Central America. L. alba exhibits variability among its different accessions, showing differences in morphology and in the composition of its essential oil. This study evaluated the phenolic profiles and the antioxidant activities of seven different accessions of L. alba. The seven accessions of L. alba studied exhibited an important phenolic content, and all accessions demonstrated antioxidant activity wit...

  17. La morera (Morus alba, Linn.: una especie de interés para la alimentación animal Mulberry (Morus alba, Linn.: a species of interest for livestock production

    Directory of Open Access Journals (Sweden)

    G.J Martín

    2007-12-01

    Full Text Available La morera es una planta multipropósito originaria de China. Durante más de 5 000 años ha sido el único alimento del gusano de seda, debido a sus excelentes cualidades nutricionales, entre las que se destaca su alto contenido de proteína y de energía. Además, es utilizada en varias regiones del mundo para la alimentación del ganado. Por tales motivos, en el año 1996 los investigadores de la Estación Experimental de Pastos y Forrajes "Indio Hatuey", tomando en consideración la necesidad de buscar nuevas fuentes de forrajes para la alimentación animal, reconsideraron la posibilidad de introducir al país cuatro variedades de morera procedentes de Costa Rica, sobre la base de las relaciones establecidas con el Centro Agronómico Tropical de Investigación y Enseñanza (CATIE de ese país, con el fin de evaluar su comportamiento en distintas condiciones edafoclimáticas y realizar ensayos con diferentes especies de animales. Para cumplir estos objetivos se formó un grupo multidisciplinario en el que participaron especialistas de varias instituciones y se realizaron investigaciones que han permitido caracterizar la composición bromatológica y fitoquímica de las variedades introducidas, conocer el efecto de los factores de manejo agronómico para determinar el potencial de producción de biomasa total y comestible, así como determinar el consumo, la digestibilidad y la respuesta animal. De esta manera, se demostró que dicha planta tiene una gran capacidad adaptativa a diferentes condiciones edafoclimáticas en todo el país; puede producir entre 10 y 12 t de MS/ha/año (biomasa comestible, posee una digestibilidad de la MS superior al 80%, y los valores de ganancia de peso vivo y de producción de leche son similares a los obtenidos con la utilización de concentrados importados. En la actualidad la morera se ha convertido en una especie conocida y utilizada en todo el país por los empresarios y los campesinos y es de gran aceptación, sobre todo para alimentar especies menores en los diferentes subprogramas pecuarios de la agricultura urbana.Mulberry is a multipurpose plant which originated in China. For more than 5 000 years it has been the only feedstuff for the silkworm, due to its excellent nutritional qualities, especially its high content of protein and energy. In addition, it is used in several regions of the world for livestock feeding. For such reasons, in 1996 the researchers of the Experimental Station of Pastures and Forages "Indio Hatuey", taking into consideration the need to search for new sources of forages for animal feeding, re-considered the possibility of introducing to the country four mulberry varieties from Costa Rica, based on relationships established with the Tropical Agronomic Center of Research and Training (CATIE from that country, with the objective of evaluating their performance under different edaphoclimatic conditions and carrying out trials with different animal species. In order to achieve these objectives a multidisciplinary group was created involving specialists from several institutions and studies were performed which have allowed to characterize the bromatological and phytochemical composition of the varieties introduced, learn the effect of the factors of agronomic management for determining the production potential of total and edible biomass, as well as the intake, digestibility and animal response. Thus, this plant was demonstrated to have great adaptive capacity to different edaphoclimatic conditions all over the country; it can produce between 10 and 12 t DM/ha/year (edible biomass, has a DM digestibility higher than 80%, and the values of live weight gain and milk production are similar to the ones obtained with the use of imported concentrates. Nowadays mulberry has become a species known and used all over the country by entrepreneurs and farmers and has great acceptance, especially for feeding small species in the different livestock subprograms of urban agriculture.

  18. Sustitución de alimento concentrado, con morera fresca (Morus alba, en la dieta de vacas lecheras

    Directory of Open Access Journals (Sweden)

    Carlos Boschini

    2003-01-01

    Full Text Available Se experimentaron cuatro dietas balanceadas con sustitución de alimento concentrado por la inclusión de 0, 20, 40 y 60% de materia seca de hojas de morera de 84 días. El consumo de materia seca total fue de 3,12 a 3,24% con relación al peso vivo. No se apreciaron diferencias importantes (P≥0,05 en el consumo total de MS entre vacas dentro de un mismo tratamiento. En la dieta sin morera (0%, el forraje constituyó el 42% y en la dieta con 60% de morera constituyó el 86% de la materia seca. El consumo de morera fue cercano a 2% en la dieta con 60%. La tasa de sustitución fue de 677 g/animal- /día de materia seca de concentrado por cada kilogramo de materia seca de morera agregada a la dieta, más la sustitución de 323 g/animal/día del sorgo negro forrajero y de la soja en mezcla para los dietas que contienen esta última. El reemplazo de proteína fue de 576 g/animal/día del concentrado y 424 g/animal/día del sorgo negro forrajero y soja por cada kilogramo de proteína cruda de morera adicionada. Cada megacalor ía de energía digestible de morera agregada a la dieta sustituyó 0,675 mcal/animal/día de energía del concentrado y 0,325 mcal/animal/día de la mezcla sorgo negro forrajero y soja. Se encontraron diferencias (P0,05 entre el peso inicial y final de los animales y las variaciones de producción de leche diaria fueron de pequeña magnitud dentro de dietas.

  19. Energy Sufficiency of Feed Containing Fermented Mulberry Leaves (Morus alba) Determined by measurement of Glucose, Abdominal Fat and Feed Intake

    OpenAIRE

    Has, Hamdan; V. D.Yunianto; Sukamto, B.

    2013-01-01

    This study was conducted to determine the effect of using rumen liquid fermented mulberry leaves in boiler???s diet on feed consumption, blood glucose, and abdominal fat. This research used 100 of day old CP 707 broiler chicks, as well as concentrate, fermented mulberry leaves and other feed stuffs. The experiment was carried out according to completely randomized design consisted of five treatments, i.e. T0 (control), T1 (10% mulberry leaf), T2 (10% fermented mulberry leaf), T3 (20% mulberry...

  20. EVALUACIÓN DE CUATRO NIVELES DE MORERA (Morus alba EN ENGORDE DE CONEJO BAJO NORMATIVA ORGÁNICA

    Directory of Open Access Journals (Sweden)

    David Mora-Valverde

    2012-01-01

    Full Text Available El objetivo de este estudio fue evaluar dietas para conejos cuyo componente principal fuese la morera. Se desarrolló una evaluación nutricional en la provincia de Cartago, Costa Rica, bajo un sistema de manejo y alimentación acordes con la normativa de producción orgánica; utilizando un sistema de alojamiento diferenciado. Se evaluaron cuatro niveles de inclusión de harina de morera dentro de la ración completa; D1: 45%; D2: 55%; D3: 65% y D4: 75%. Se observaron ganancias de peso diaria en el orden de 17,65 g/d (promedio general. Las dietas D1 y D2, presentaron los parámetros de crecimiento más altos respecto a las dietas con niveles de 65% (D3 y 75 % (D4 de inclusión. Las ganancias diarias de peso obtenidas presentaron valores de D1: 17,26 g/d, D2: 21,57 g/d, D3: 14,77 g/d y D4: 17,00 g/d. En relación a la conversión alimenticia, los conejos de las dietas D1 y D2 fueron más eficientes en el uso del alimento para fines de crecimiento que los conejos de las dietas D3 y D4 (P<0,05. El costo de alimento total para llevar a los animales hasta peso de mercado para cada tratamiento fue D1: 2,26 US$/kg; D2: 2,17 US$/kg, D3: 2,49 US$/kg y D4: 2,44 US$/kg. Se estimó que el tiempo de engorde y de salida a mercado se prolongaría entre 2 a 3,5 veces con respecto a un periodo típico de engorde utilizando únicamente concentrados, existiendo un equilibrio respecto a la reducción en los costos de alimentación entre el 45 y 50%.

  1. Lead in the soil-mulberry (Morus alba L.)-silkworm (Bombyx mori) food chain: translocation and detoxification.

    Science.gov (United States)

    Zhou, Lingyun; Zhao, Ye; Wang, Shuifeng; Han, Shasha; Liu, Jing

    2015-06-01

    The translocation of lead (Pb) in the soil-mulberry-silkworm food chain and the process of Pb detoxification in the mulberry-silkworm chain were investigated. The amount of Pb in mulberry, silkworm, feces and silk increased in a dose-responsive manner to the Pb contents in the soils. Mulberry roots sequestered most of the Pb, ranging from 230.78 to 1209.25 mg kg(-1). Over 92% of the Pb in the mulberry leaves was deposited in the cell wall, and 95.29-95.57% of the Pb in the mulberry leaves was integrated with oxalic acid, pectates and protein, and it had low bioavailability. The Pb concentrations in the silkworm feces were 4.50-4.64 times higher than those in the leaves. The synthesis of metallothioneins in three tissues of the silkworms was induced to achieve Pb homeostasis under Pb stress. These results indicated the mechanism involved in Pb transfer along the food chain was controlled by the detoxification of Pb in different trophic levels. Planting mulberry and rearing silkworm could be a promising approach for the remediation of Pb-polluted soils due to the Pb tolerance of mulberry and silkworm.

  2. The mulberry plant (Morus alba), tool for combating desertification the experience of the sericulture promotion and development association (Ghana)

    Energy Technology Data Exchange (ETDEWEB)

    Ntaanu, P. K.

    2009-07-01

    Desertification may be defined as the loss of fertility of the land in semi-arid and sub-humid regions of the world. Its causes are broadly categorized into natural and anthropogenic and includes soil erosion (wind, water), soil exploitation (nutrients depletion), salination (primary and secondary) and others including soil compaction, contamination, etc. Desertification is on-going in all parts of Ghana but it is quite extensive and more visible in the north, upper-east and upper-west regions of the country. this area is desert prone and is in the guinea savannah agro-ecological zone. It occupies about 40% of the total area of the country. The desert prone region otherwise termed as semi-arid Ghana is located between latitude eight degrees north and eleven degrees north of the equator. (Author) 2 refs.

  3. CONSUMO DE MORERA (Morus alba) FRESCA MEZCLADA CON ENSILAJE DE MAÍZ POR EL GANADO JERSEY EN CRECIMIENTO

    OpenAIRE

    David Mora-Valverde

    2010-01-01

    Con el objetivo de determinar la efectividad de la mezcla de morera con ensilaje de maíz en novillas Jersey, se llevó a cabo un estudio en la Estación Experimental Alfredo Volio Mata de la Universidad de Costa Rica localizada en Ochomogo, Cartago, Costa Rica en el año 2007. Se ofrecieron tres mezclas de morera fresca finamente picada con ensilaje de maíz en proporciones morera:ensilaje de 36:64, 46:54 y 56:44. Se utilizaron animales hembra raza Jersey entre los 200 y los 311 kg. Se recolectar...

  4. RENDIMIENTO Y CALIDAD DE LA MORERA (Morus alba FERTILIZADA CON NITRÓGENO, FÓSFORO Y POTASIO

    Directory of Open Access Journals (Sweden)

    Carlos Boschini-Figueroa

    2009-01-01

    suelo y se cosechó a los 84 días de rebrote. El rendimiento anual de materia seca mostró diferencias (p0,05, la respuesta fue similar con diferentes cantidades de fósforo y potasio. La concentración de fibra neutro detergente se afectó (p0,05 la fibra ácido detergente; sin embargo, se observó que al incrementar el nitrógeno la hemicelulosa decreció (p0,05.

  5. Comportamiento productivo de cerdos en ceba alimentados con follaje fresco de Morus alba como sustituto parcial del concentrado

    Directory of Open Access Journals (Sweden)

    Contino Yuván| Ojeda Félix | Herrera Rafael | Altunaga Nancy | Pérez Guadalupe

    2008-08-01

    Full Text Available El objetivo de esta investigación consistió en evaluar la respuestaproductiva en la etapa de ceba en cerdos mestizos al emplear el follaje de morera fresca (hojas y tallos tiernos como sustituto parcial del concentrado comercial. La investigación se efectuó en el Módulo de Investigación-Producción Porcina de la EEPF ”Indio Hatuey” Se evaluaron las ganancias de peso vivo de cerdos en crecimiento desde los 30 kg hasta los 90 kg. El pesaje se realizó cada 14 días, con la determinación del peso vivo promedio, la ganancia media diaria, y la conversión alimentaría. Para el procesamiento se empleó el paquete estadístico SPSS versión 10.1. Se obtuvo un incremento de las ganancias medias diarias con el aumento del peso vivo y una mejor eficiencia en la dieta total por la utilización de lamorera. Los resultados demuestran la factibilidad económica de estaalternativa alimentaria

  6. Sustitución de alimento concentrado, con morera fresca (Morus alba), en la dieta de vacas lecheras

    OpenAIRE

    Carlos Boschini

    2003-01-01

    Se experimentaron cuatro dietas balanceadas con sustitución de alimento concentrado por la inclusión de 0, 20, 40 y 60% de materia seca de hojas de morera de 84 días. El consumo de materia seca total fue de 3,12 a 3,24% con relación al peso vivo. No se apreciaron diferencias importantes (P≥0,05) en el consumo total de MS entre vacas dentro de un mismo tratamiento. En la dieta sin morera (0%), el forraje constituyó el 42% y en la dieta con 60% de morera constituyó...

  7. Isolation and structure elucidation of a new linoleiyl glycoside and flavones from the stem bark of Morus alba L.

    Directory of Open Access Journals (Sweden)

    Abuzer Ali

    2016-12-01

    Conclusion: The present work reports the isolation and characterization of new phytoconstituents which could be used as chromatographic fingerprinting markers for standardization and quality control parameters.

  8. Produção da amoreira-preta 'Tupy' sob diferentes épocas de poda Productive of 'Tupy' blackberry under different pruning time

    Directory of Open Access Journals (Sweden)

    Marcelo Angelo Campagnolo

    2012-02-01

    Full Text Available A amora-preta é uma opção importante para fruticultura paranaense, porém não há informações a respeito do cultivo dessa frutífera nas condições subtropicais do Estado. O objetivo deste trabalho foi avaliar a influência da época de poda na produção da amoreira-preta 'Tupy'. O trabalho foi realizado em um pomar comercial, conduzido em sistema agroecológico. O delineamento experimental foi em blocos ao acaso, com quatro blocos e seis tratamentos (podas quinzenais realizadas durante o inverno. Em cada parcela, constituída de três plantas úteis, foram coletados dados fenológicos, produtivos e físico-químicos no ciclo de produção 2008/09 e 2009/10. Podas efetuadas no início de julho são as mais indicadas e podas tardias podem prejudicar o desempenho produtivo das amoreiras-pretas 'Tupy' no oeste do Paraná.The blackberry is an important option to fruit growers in Paraná State. However, there aren't trials performed in subtropical area in that State. The objective of this research was to evaluate the pruning time effects at season production of 'Tupy' blackberry. The research was conducted in a commercial orchard, in an agroecological system. The experimental design was randomized blocks with four blocks and six treatments (pruning time by fifteen days during the winter pruning. In each plot, which was consisted of three plants, it was collected phenological, productive and physical-chemical production cycle in 2008/09 and 2009/10. Pruning made in early July are the most suitable and late pruning can harm the productive performance of blackberry 'Tupy' in western Paraná.

  9. Alba is first heavy North Sea crude

    Energy Technology Data Exchange (ETDEWEB)

    1991-05-27

    The development of the Alba oil field will constitute two North Sea firsts: the first Eocene reservoir developed, and the first development to handle heavy crude. The field was discovered in Block 16/26 of the North Sea's U.K. sector in 1984. The Alba field is in the heart of the North Sea, about midway between the northern fields of the East Shetlands basin and the southern Fulmar and Argyll fields. About 250 million bbl of the estimated 1 billion bbl reservoir of 20{degrees} gravity crude is believed recoverable.

  10. Crescimento in vitro de amoreira-preta: efeito de reguladores de crescimento e da cultivar In vitro blackberry growing: effect of growth regulators and cultivar

    Directory of Open Access Journals (Sweden)

    Fabíola Villa

    2008-12-01

    Full Text Available Desenvolveu-se, este trabalho, no intuito de aprimorar técnicas de propagação in vitro de amoreira-preta. Testou-se em um experimento a influência de cinco diferentes concentrações de ANA (0; 0,1; 0,5; 1,0 e 1,5 mg L-1 e cinco de GA3 (0; 2; 4; 6 e 8 mg L-1, adicionadas ao meio de cultura MS, sob a amoreira-preta cultivar Ébano e; num segundo experimento testaram-se seis diferentes concentrações de ANA (0; 0,1; 0,5; 1,0; 1,5 e 2,0 mg L-1 e duas cultivares de amoreira-preta (Tupy e Brazos, no crescimento in vitro de plântulas. Segmentos nodais com 2 cm, de plântulas preestabelecidas in vitro, foram excisadas e inoculadas, em meio MS. O experimento foi inteiramente casualisado, utilizando-se três explantes por repetição e quatro repetições por tratamento. O pH do meio foi ajustado para 5,8 depois da adição de 6 g L-1 de ágar e 30 g L-1 de sacarose, ocorrendo depois a autoclavagem a 121ºC e 1 atm por 20 minutos. Após a inoculação, os tubos de ensaio foram mantidos por 60 dias, em sala de crescimento a 27 ± 1ºC, irradiância de 35 mmol.m-2.s-1 e fotoperíodo de 16 horas, avaliando-se assim o número de folhas, número de raízes, comprimento da maior raiz, comprimento da parte aérea, peso da matéria fresca e seca da parte aérea. Altas concentrações de GA3 associadas a baixas de ANA promoveram maior comprimento da parte aérea da amoreira-preta, cultivar Ébano. Maior comprimento da parte aérea de 'Brazos' foi verificado na presença de 1,0 mg L-1 de ANA. Verificou-se surgimento de calos na cultivar Ébano em todas as concentrações de GA3 associadas a 0,5-1,5 mg L-1 de ANA e nas cultivares Tupy e Brazos em todas as concentrações de ANA. Melhores resultados na micropropagação da amoreira-preta cultivares Tupy e Ébano foram obtidos com a adição de 1,0 mg L-1 de ANA e melhores resultados no enraizamento da amoreira-preta cultivar Ébano foram obtidos com baixas concentrações de ANA e na ausência de GA3.This work

  11. FLAVANONES FROM THE WOOD OF Morus nigra WITH CYTOTOXIC ACTIVITY

    Directory of Open Access Journals (Sweden)

    Ferlinahayati Ferlinahayati

    2013-12-01

    Full Text Available Two flavanone derivatives, norartocarpanone (1 and euchrenone a7 (2 had been isolated for the first time from the methanol extract of the wood of Morus nigra. The structures of these compounds were determined base on spectral evidence, including UV, IR, and NMR. The first compound also confirmed by comparison with the reported data. Cytotoxic properties of these compounds were evaluated against murine leukemia P-388 cells. Euchrenone a7 (2 was found more cytotoxic than norartocarpanone (1 with their IC50 7.8 and 12.7 mg/mL respectively.

  12. Chalcone dimethylallyltransferase from Morus nigra cell cultures. Substrate specificity studies.

    Science.gov (United States)

    Vitali, Alberto; Giardina, Bruno; Delle Monache, Giuliano; Rocca, Filippo; Silvestrini, Andrea; Tafi, Andrea; Botta, Bruno

    2004-01-16

    A new prenyltransferase (PT) enzyme derived from the microsomal fractions of cell cultures of Morus nigra was shown to be able to prenylate exclusively chalcones with a 2',4'-dihydroxy substitution and the isoflavone genistein. Computational studies were performed to shed some light on the relationship between the structure of the substrate and the enzymatic activity. PT requires divalent cations, particularly Mg(2+), to be effective. The apparent K(m) values for gamma,gamma-dimethylallyldiphosphate and 2',4'-dihydroxychalcone were 63 and 142 microM, respectively. The maximum activity of the enzyme was expressed during the first 10 days of cell growth.

  13. THE STUDY OF MORPHOLOGICAL AND ANATOMICAL CHARACTERISTICS OF STEMS OF MORUS NIGRA

    OpenAIRE

    Yu. A. Vakhrusheva; I. I. Kharchenko; A. S. Nikitina; E. T. Oganesyan

    2015-01-01

    Until now, plants have been the main source of new drugs. Morus nigra, which contains a rich complex of biologically active substances necessary for an organism (vitamins, anthocyanins, macro- and microelements, organic acids) is of great interest. We have conducted morphological and anatomical studies of stems of Morus nigra, identified the main diagnostic characters of the studied medicinal plants. Experimental studies have shown that the stems of Morus nigra are characterized by the presen...

  14. [Introduction trial of medicine mulberry (Morus nigra) in Chongqing].

    Science.gov (United States)

    Zeng, Qi-Wei; Zhang, Chao; Chen, Hong-Yu; Ding, Tian-Long; Zhao, Ai-Chun; Xiang, Zhong-Huai; He, Ning-Jia

    2016-04-01

    Medicine mulberry (Morus nigra) mainly distributed in southern areas of Xinjiang Uighur Autonomous Region and introduced by grafting, is a unique Morus species, whose plant number is little. As a traditional herbal medicine, medicine mulberry with high levels of secondary metabolites has important values of scientific research and utilization. In order to solve the introduction problems for medicine mulberry, we have established its rapid propagation system through tissue culture since 2011. The shoots of medicine mulberry through tissue culture were transplanted into the field to carry out an introduction experiment. Here, we firstly reported that the growth status and pest and disease occurrence of medicine mulberry in the field of Chongqing and found that the medicine mulberry through tissue culture had well-developed root system, it showed better growth than medicine mulberry by grafting technique, and Pseudodendrothrips moil was a major pest of medicine mulberry. The introduction technique for medicine mulberry established successfully in this study could lay the foundation for large-scale cultivation and high efficiency utilization of medicine mulberry. Copyright© by the Chinese Pharmaceutical Association.

  15. Effect of geographical distributions on the nutrient composition, phytochemical profile and antioxidant activity of Morus nigra

    National Research Council Canada - National Science Library

    Khattak, Khanzadi Fatima; Rahman, Tajur Rahman

    2015-01-01

    .... The current study was carried out to see the effect of geographical locations on the nutrient composition, mineral contents, phytochemical profile and free radical scavenging activity of Morus nigra fruit...

  16. STRUCTURES OF TWO NEW BENZOFURAN DERIVATIVES FROM THE BARK OF MULBERRY TREE (MORUS MACROURA MIQ.)

    Institute of Scientific and Technical Information of China (English)

    SHENG-GUO SUN; RUO-YUN CHEN; DE-QUAN YU

    2001-01-01

    Two new benzofuran derivatives, macrourins A (1) and B (2), together with two known stilbene derivatives, were isolated from the barks of Morus macroura Miq. Their structures were elucidated by means of spectroscopic evidence.

  17. A new benzofuranolignan and a new flavonol derivative from the stem of Morus australis

    Institute of Scientific and Technical Information of China (English)

    Qing Jian Zhang; Di Zao Li; Ruo Yun Chen; De Quan Yu

    2008-01-01

    A new benzofuranolignan austrafuran A(1)and a new flavonol derivative 5,7,2',4'-tetrahydroxy-3-methoxyflavone(2)were isolated from the stem of Morus australis.Their structures were determined on the basis of spectroscopic evidences.

  18. MorusDB: a resource for mulberry genomics and genome biology.

    Science.gov (United States)

    Li, Tian; Qi, Xiwu; Zeng, Qiwei; Xiang, Zhonghuai; He, Ningjia

    2014-01-01

    Mulberry is an important cultivated plant that has received the attention of biologists interested in sericulture and plant-insect interaction. Morus notabilis, a wild mulberry species with a minimal chromosome number is an ideal material for whole-genome sequencing and assembly. The genome and transcriptome of M. notabilis were sequenced and analyzed. In this article, a web-based and open-access database, the Morus Genome Database (MorusDB), was developed to enable easy-to-access and data mining. The MorusDB provides an integrated data source and an easy accession of mulberry large-scale genomic sequencing and assembly, predicted genes and functional annotations, expressed sequence tags (ESTs), transposable elements (TEs), Gene Ontology (GO) terms, horizontal gene transfers between mulberry and silkworm and ortholog and paralog groups. Transcriptome sequencing data for M. notabilis root, leaf, bark, winter bud and male flower can also be searched and downloaded. Furthermore, MorusDB provides an analytical workbench with some built-in tools and pipelines, such as BLAST, Search GO, Mulberry GO and Mulberry GBrowse, to facilitate genomic studies and comparative genomics. The MorusDB provides important genomic resources for scientists working with mulberry and other Moraceae species, which include many important fruit crops. Designed as a basic platform and accompanied by the SilkDB, MorusDB strives to be a comprehensive platform for the silkworm-mulberry interaction studies. Database URL: http://morus.swu.edu.cn/morusdb. © The Author(s) 2014. Published by Oxford University Press.

  19. Miguel de Unamuno y Santiago Alba

    Directory of Open Access Journals (Sweden)

    José María Marín Arce

    1992-01-01

    Full Text Available A partir de la correspondencia privada entre Unamuno y Alba, reconstruimos las relaciones que durante más de treinta años tuvieron el que fuera rector de la Univerdad de Salamanca, figura destacada de la «generación del 98», y el político zamorano, numerosas veces ministro y jefe de la izquierda liberal.

  20. ECLALBATIN, A TRITERPENE SAPONIN FROM ECLIPTA ALBA

    Institute of Scientific and Technical Information of China (English)

    R. K. UPADHYAY; M. B. PANDEY; R. N. JHA; V. B. PANDEY

    2001-01-01

    From the whole plant of Eclipta alba, a new triterpene saponin, named eclalbatin, together with α-amyrin, ursolic acid and oleanolic acid were isolated. The structure of eclalbatin has been established as 3-0-β-D-glucopyranosyl-3-β-hydroxy-olean-12-en-28-oic acid, 28-0-β-D-arabino-pyranoside (1) on the basis of chemical and spectral data.

  1. Poda drástica de verão e produção de cultivares de amoreira-preta em região subtropical

    Directory of Open Access Journals (Sweden)

    Maraisa Hellen Tadeu

    2015-02-01

    Full Text Available O objetivo deste trabalho foi avaliar o manejo de podas em cultivares de amoreira-preta (Rubus spp., submetidas à poda drástica de verão, em regiões subtropicais. O experimento foi conduzido em blocos ao acaso, em arranjo fatorial 2x7 (podas x cultivares, no Município de Lavras, MG. Foram utilizadas as cultivares Comanche, Caingangue, Choctaw, Tupy, Guarani, Brazos e Cherokee, que receberam poda convencional (controle e poda drástica de verão. Foram avaliados dados fenológicos, vegetativos e produtivos nos ciclos de produção 2012/2013 e 2013/2014, além de características físico-químicas dos frutos e da incidência de doenças, no último ciclo de produção. A poda drástica de verão aumenta a produção das cultivares em regiões subtropicais. Não há diferença na qualidade dos frutos colhidos de plantas submetidas aos sistemas de poda avaliados. A poda drástica de verão não é eficiente na redução ou no controle de doenças.

  2. Qualidade de luz e fitorreguladores na multiplicação e enraizamento in vitro da amoreira-preta 'Xavante' Light quality and growth regulators on in vitro multiplication and rooting of blackberry 'Xavante'

    Directory of Open Access Journals (Sweden)

    Mateus da Silveira Pasa

    2012-08-01

    Full Text Available A técnica de micropropagação apresenta diversas vantagens em relação aos métodos tradicionalmente utilizados na propagação da amoreira-preta, especialmente quanto à maior sanidade das mudas e maior rapidez na obtenção de novas plantas. O objetivo deste trabalho foi verificar a influência dos fitorreguladores 6-benzilaminopurina (BAP e ácido indolbutírico (AIB, aliados à qualidade de luz, na multiplicação e enraizamento in vitro, respectivamente, da amoreira-preta cultivar 'Xavante'. O delineamento experimental utilizado foi inteiramente casualizado. Os tratamentos constituíram-se de diferentes níveis de qualidade de luz (azul, vermelha, branca e ausência de luz combinadas com ausência ou presença dos fitorreguladores AIB (0,5mg L-1 e BAP (0,8mg L-1 no meio de cultivo. A utilização de BAP aumenta o número de brotações, gemas e folhas da amoreira-preta 'Xavante' e, dessa forma, é indicada para sua multiplicação in vitro. A utilização de AIB não é necessária no enraizamento in vitro da amoreira-preta 'Xavante', indicando que essa cultivar apresenta bom enraizamento natural.There are a lot of advantages using micropropagation technique over the methods traditionally used in blackberry propagation especially concerning health of seedlings and speed in getting new plants. The aim of this study was to investigate the influence of bioregulators 6-benzylaminopurine (BAP and indol-3-butyric acid (IBA combined with light quality on in vitro multiplication and rooting, respectively, of blackberry 'Xavante'. The experimental design was completely randomized. The treatments consisted of different levels of light quality (blue, red, white and absence of light combined with the absence or presence of bioregulators IBA (0.5mg L-1 and BAP (0.8mg L-1 in the culture medium. The use of BAP increases the number of new shoots, buds and leaves of blackberry, 'Xavante' and thus is suitable for its in vitro propagation. The use of IBA

  3. The Alba ray tracing code: ART

    Science.gov (United States)

    Nicolas, Josep; Barla, Alessandro; Juanhuix, Jordi

    2013-09-01

    The Alba ray tracing code (ART) is a suite of Matlab functions and tools for the ray tracing simulation of x-ray beamlines. The code is structured in different layers, which allow its usage as part of optimization routines as well as an easy control from a graphical user interface. Additional tools for slope error handling and for grating efficiency calculations are also included. Generic characteristics of ART include the accumulation of rays to improve statistics without memory limitations, and still providing normalized values of flux and resolution in physically meaningful units.

  4. THE STUDY OF MORPHOLOGICAL AND ANATOMICAL CHARACTERISTICS OF STEMS OF MORUS NIGRA

    Directory of Open Access Journals (Sweden)

    Yu. A. Vakhrusheva

    2015-01-01

    Full Text Available Until now, plants have been the main source of new drugs. Morus nigra, which contains a rich complex of biologically active substances necessary for an organism (vitamins, anthocyanins, macro- and microelements, organic acids is of great interest. We have conducted morphological and anatomical studies of stems of Morus nigra, identified the main diagnostic characters of the studied medicinal plants. Experimental studies have shown that the stems of Morus nigra are characterized by the presence of thick-walled epidermal cells with slightly convoluted walls and a large number of ordinary thick-walled fuzz.

  5. Progress of the Synchrotron Light Source ALBA

    CERN Document Server

    Einfeld, Dieter; Campmany, Josep; Muñoz, Marc; Pont, Montserrat

    2005-01-01

    ALBA will be a third generation synchrotron light source built in Spain near Barcelona. Commissioning of the storage ring is foreseen to start at the end of 2008. The design phase of ALBA is almost completed and the first components are ready to be ordered. A 100 MeV LINAC will inject electrons into a nominal energy booster synchrotron of similar circumference as the storage ring, so that both accelerators will share the same tunnel. The storage ring, working at 3 GeV with a circumference of 268.8 m, has been designed for a maximum current of 400 mA. The lattice is based on an extended DBA structure and has a nominal emittance of 4 nm.rad.The machine has a four fold symmetry with 4 long straight sections (8 m), 12 medium (4.2 m) and 8 short (2.6 m). This report concentrates on recent design developments, component choices and current status. Another paper at this conference deals with accelerator physics issues.

  6. Fenologia e produção de cultivares de amoreira-preta em sistema agroecológico Phenology and production of blackberry cultivars in agroecological system

    Directory of Open Access Journals (Sweden)

    Luis Eduardo Corrêa Antunes

    2010-09-01

    Full Text Available A amoreira-preta é uma espécie de exploração recente no Brasil. Nos últimos ano, s tem sido dada especial atenção ao cultivo orgânico ou agroecológico dessa espécie. O objetivo deste trabalho foi verificar o comportamento fenológico e produtivo das cultivares de amora-preta 'Tupy', 'Guarani', 'Caingangue', 'Cherokee' e 'Brazos', e das seleções denominadas 'seleção 97' e 'seleção 787' na região de Pelotas, Rio Grande do Sul(RS, em sistema agroecológico. O trabalho foi realizado na Estação Experimental de Cascata (EEC, Embrapa Clima Temperado. As plantas foram dispostas no pomar, em blocos varietais aleatórios, com 10 plantas por genótipo, perfazendo quatro linhas com duas cultivares por linha. Foram observadas as datas de início da floração e fim da floração, início e final de colheita, massa (gramas e número de frutos por planta, teor de sólidos solúveis totais (°Brix, produção média por planta (g pl-1 e produtividade estimada por hectare (kg ha-1. Para análise das características dos frutos, a média de cada ano de avaliação (três foi considerada como uma repetição. Na região de Pelotas-RS, é viável o cultivo agroecológico de amoreira-preta. Não há diferenças de produção, produtividade, número de frutos e teores de sólidos solúveis totais entre os genótipos estudados.The aim of this research was to evaluate yield and quality of blackberry cultivars Tupy, Guarani, Caingangue, Cherokee and Brazos, and the 'selection 97' and 'selection 787' at Pelotas, in the Southern of Rio Grande do Sul State, under agroecological system. The plants were available in blocks varietal randomized in the orchard, with 10 plants per row, four lines with two cultivars per line. For statistic analysis of the fruit characteristics each year of evaluation (three was considered as one repetition. The characteristics evaluate were the beginning and end of flowering and harvest, mass (g, number of fruit per plant

  7. Antioxidant Effect of Lippia alba (Miller N. E. Brown

    Directory of Open Access Journals (Sweden)

    Claire E. Chies

    2013-09-01

    Full Text Available Lippia alba is a shrub found in all regions of Brazil and other countries in South and Central America. L. alba exhibits variability among its different accessions, showing differences in morphology and in the composition of its essential oil. This study evaluated the phenolic profiles and the antioxidant activities of seven different accessions of L. alba. The seven accessions of L. alba studied exhibited an important phenolic content, and all accessions demonstrated antioxidant activity with different efficacies. The main flavonoids in all accessions were apigenin, luteolin, naringin and rutin. The Santa Vitória do Palmar accession exhibited higher naringin and total phenolic content. This extract was able to reduce hydrogen peroxide-induced oxidative damage in tissue homogenates of cerebellum, cerebral cortex, hippocampus and liver of Wistar rats.

  8. Antioxidant Effect of Lippia alba (Miller) N. E. Brown.

    Science.gov (United States)

    Chies, Claire E; Branco, Cátia S; Scola, Gustavo; Agostini, Fabiana; Gower, Adriana E; Salvador, Mirian

    2013-09-26

    Lippia alba is a shrub found in all regions of Brazil and other countries in South and Central America. L. alba exhibits variability among its different accessions, showing differences in morphology and in the composition of its essential oil. This study evaluated the phenolic profiles and the antioxidant activities of seven different accessions of L. alba. The seven accessions of L. alba studied exhibited an important phenolic content, and all accessions demonstrated antioxidant activity with different efficacies. The main flavonoids in all accessions were apigenin, luteolin, naringin and rutin. The Santa Vitória do Palmar accession exhibited higher naringin and total phenolic content. This extract was able to reduce hydrogen peroxide-induced oxidative damage in tissue homogenates of cerebellum, cerebral cortex, hippocampus and liver of Wistar rats.

  9. Antihepatotoxic activity of eclipta alba, tephrosia purpurea and boerhaavia diffusa.

    Science.gov (United States)

    Murthy, V N; Reddy, B P; Venkateshwarlu, V; Kokate, C K

    1992-01-01

    Alcoholic and chloroform extracts of E. albaT. purpurea and B. diffusa were screened for antihepatotoxic activity. The extracts were given after the liver was damaged with CCl4. Liver function was assessed based on liver to boy weight ratio, pentobarbitone sleep time, serum levels of transaminase (SGPT, SGOT), alkaline phosphatase (SALP) and bilirubin. Alcoholic extract of E. alba was found to have good antihepatotoxic activity.

  10. Revisión taxonómica del complejo Centaurea alba L. (Asteraceae) en la Península Ibérica

    OpenAIRE

    López, Eusebio; Devesa Alcaraz, Juan Antonio

    2011-01-01

    Taxonomic revision of the Centaurea alba L. complex (Asteraceae) in the Iberian Peninsula.- A taxonomic revision of the Centaurea alba L. complex (Centaurea L. sect. Centaurea) in the Iberian Peninsula is presented, which is represented by two species, C. alba and C. costae Willk. Three subspecies of C. alba with reasonably well-defined areas are recognized: C. alba subsp. alba, with three varieties –alba, macrocephala Pau and latronum (Pau) E. López & Devesa-, C. alba subsp. aris...

  11. Revisión taxonómica del complejo Centaurea alba L. (Asteraceae) en la Península Ibérica

    OpenAIRE

    Devesa, J. A.; López, E.

    2011-01-01

    Taxonomic revision of the Centaurea alba L. complex (Asteraceae) in the Iberian Peninsula.- A taxonomic revision of the Centaurea alba L. complex (Centaurea L. sect. Centaurea) in the Iberian Peninsula is presented, which is represented by two species, C. alba and C. costae Willk. Three subspecies of C. alba with reasonably well-defined areas are recognized: C. alba subsp. alba, with three varieties –alba, macrocephala Pau and latronum (Pau) E. López & Devesa-, C. alba subsp. aristifera (Pau...

  12. Extracts of Morus nigra L. Leaves Standardized in Chlorogenic Acid, Rutin and Isoquercitrin: Tyrosinase Inhibition and Cytotoxicity

    National Research Council Canada - National Science Library

    de Freitas, Marcela Medeiros; Fontes, Pedro Ribeiro; Souza, Paula Monteiro; William Fagg, Christopher; Neves Silva Guerra, Eliete; de Medeiros Nóbrega, Yanna Karla; Silveira, Damaris; Fonseca-Bazzo, Yris; Simeoni, Luiz Alberto; Homem-de-Mello, Maurício; Oliveira Magalhães, Pérola

    2016-01-01

    .... Thus, this project aimed to study the inhibitory activity of tyrosinase by extracts from Morus nigra leaves as well as the characterization of its chromatographic profile and cytotoxicity in order...

  13. Effect of Morus nigra aqueous extract treatment on the maternal-fetal outcome, oxidative stress status and lipid profile of streptozotocin-induced diabetic rats

    OpenAIRE

    Volpato, Gustavo Tadeu; Calderon, Iracema Mattos Paranhos; Sinzato, S. [UNESP; de Campos, Kleber Eduardo; Rudge, Marilza Vieira Cunha [UNESP; Damasceno,Débora Cristina

    2011-01-01

    Ethnopharmacological relevance: Morus nigra, commonly known as black mulberry, is widely used in Brazilian folk medicine for the diabetes treatment.Aim of this study: To evaluate the effect of Morus nigra aqueous extract treatment on maternal lipid and oxidative stress profile, reproductive outcomes, and also fetal anomaly incidence from diabetic and non-diabetic rats.Materials and methods: Diabetes was induced by streptozotocin (40 mg/kg) in virgin female Wistar rats. Morus nigra leaf aqueou...

  14. Propagação vegetativa de Lippia alba Vegetative propagation of Lippia alba

    Directory of Open Access Journals (Sweden)

    Luiz Antônio Biasi

    2003-06-01

    Full Text Available A Lippia alba é uma planta medicinal nativa da América do Sul muito utilizada pela sua propriedade calmante. No presente trabalho estudou-se a propagação vegetativa dessa espécie, visando a obtenção de uma forma eficiente de formação de mudas. Foram testados diferentes tipos de estaca (medianas com 4 folhas, medianas com 2 folhas, medianas sem folhas e apicais, tamanhos de estacas lenhosas (5, 10, 15 e 20cm e substratos (casca de arroz carbonizada, vermiculita, solo e Plantmax®. Todos os tipos de estaca apresentaram altas taxas de enraizamento, comprovando que a L. alba é uma espécie de fácil enraizamento. As estacas medianas com quatro folhas foram as que apresentaram o maior desenvolvimento radicial, ao contrário das estacas sem folhas. As estacas com duas folhas também apresentaram bom crescimento radicial, facilidade de manuseio e o dobro do rendimento no preparo das estacas em relação as com quatro folhas. Os substratos não afetaram a porcentagem de enraizamento, mas a maior massa de raízes foi obtida com casca de arroz carbonizada. O aumento do tamanho da estaca lenhosa proporcionou um aumento linear em todas as variáveis analisadas. Conclui-se que a produção de mudas de L. alba pode ser realizada com estacas semilenhosas com um par de folhas ou com estacas lenhosas com 20cm de comprimento em substratos porosos e sem necessidade de irrigação por nebulização.Lippia alba is a medicinal plant native of the South America it is used because of its sedative property. In this work the cutting propagation of this species was studied aiming at obtain an efficient protocol of plant production. Different types of cuttings were tested (middle with 4 leaves, middle with two leaves, middle without leaves and apical, length of hardwood cuttings (5, 10, 15 and 20cm and substrates (carbonized rice hulls, vermiculite, soil and Plantmax®. All types of cuttings showed high rates of rooting, confirming that L. alba is an easy to root

  15. 568 Prevalence of Sensitization to Parietaria, Pinus, Cupressus and Morus Pollens in Patients from Craic

    OpenAIRE

    Leal-Garcia, Liborio Rafael; González-Díaz, Sandra; Arias-Cruz, Alfredo; del Carmen Zarate, Maria; Garcia-Calderin, Diego; Gallego, Claudia

    2012-01-01

    Background The pollens of Cupressus, Parietaria, Pinus and Morus are important causes of allergic respiratory diseases. In a study of pollen concentration in Monterrey in 2004, these pollens occupied the first places in frequency. The objective was to determine the prevalence of sensitization to Parietaria, Pinus, Cupressus and Morus in patients from CRAIC (Regional Center of allergy and clinical immunology, Monterrey, México) Methods Is an observational, cross-comparative, double-blind study...

  16. Brazilian ground pearl damaging blackberry, raspberry and blueberry in Brazil Pérola-da-terra causando danos em amoreira-preta, framboeseira e mirtileiro no Brasil

    Directory of Open Access Journals (Sweden)

    Caio Fábio Stoffel Efrom

    2012-09-01

    Full Text Available The Brazilian ground pearl, Eurhizococcus brasiliensis (Wille, 1922 (Hemiptera: Margarodidae, is a subterranean, polyphagous scale insect native of Southern Brazil that feeds on a variety of different vineyard plant species (Vitis spp.. In this study, it is reported three new plant hosts of the species. In 2007 and 2010, infested plants were documented in the towns of Farroupilha (29°14'34"S, 51°23'20"W and Vacaria (28°26'30"S, 50°52'59"W in Rio Grande do Sul, Brazil. Specimens of the ground pearl were found in the roots of three cultivated berry plants: blackberry (Rubus spp., raspberry (Rubus idaeus L. and blueberry (Vaccinium sp.. Observed symptoms included: chlorotic leaves, gradual wasting, reduced production, and mortality. Given the increasing popularity of berry orchards in the region, this study serves as an alert for farmers to avoid establishing them in areas infested with the ground pearl and to check for the insects in the root cuttings used to establish berry crops.A pérola-da-terra Eurhizococcus brasiliensis (Wille, 1922 (Hemiptera: Margarodidae é uma cochonilha subterrânea, polífaga, nativa do sul do Brasil, que se alimenta de diversas espécies vegetais causando prejuízos, principalmente à cultura da videira (Vitis spp.. Nesta comunicação, três espécies vegetais são relatadas como novos hospedeiros da praga. Em 2007 e 2010, plantas infestadas foram registradas nos municípios de Farroupilha (29°14'34"S e 51°23'20"W e Vacaria (28°26'30"S e 50°52'59"W, RS. Exemplares da cochonilha foram encontrados em raízes de amoreira-preta (Rubus spp., framboeseira (Rubus idaeus L. e mirtileiro (Vaccinium sp.. Os sintomas observados foram folhas cloróticas, definhamento progressivo, diminuição na produção e morte das plantas. Devido à ampliação do cultivo de pequenas frutas na região, essa informação serve de alerta aos produtores para que evitem o plantio em áreas infestadas com a cochonilha e observem a presen

  17. Enraizamento e aclimatização de plantas micropropagadas de amoreira-preta cv. Brazos Rooting and acclimatization of micropropagated plants of blackberry cv. Brazos

    Directory of Open Access Journals (Sweden)

    Carolina Smanhotto Schuchovski Augusto

    2006-12-01

    Full Text Available O presente trabalho estudou as fases finais do processo de micropropagação, incluindo o enraizamento e a posterior aclimatização da amoreira-preta cv. Brazos. Os experimentos foram realizados no Laboratório de Micropropagação de Plantas da UFPR, Curitiba-PR, no período de março de 2000 a julho de 2001. No enraizamento in vitro com ou sem imersão em AIB, obteve-se mais de 95% de enraizamento nos dois tratamentos. No experimento de enraizamento ex vitro, com microestacas provenientes da multiplicação com diferentes citocininas, as taxas de enraizamento e sobrevivência foram de 100%. Na fase final, testou-se a influência da sacarose do meio de cultura de enraizamento in vitro na posterior aclimatização. Em todos os tratamentos, houve 100% de sobrevivência. Conclui-se que pode ser realizado um eficiente enraizamento in vitro sem AIB e sem a adição de sacarose ao meio de cultura, com posterior aclimatização em túnel plástico ou, ainda, realizar o enraizamento ex vitro e aclimatização em casa de vegetação com nebulização intermitente.The present work studied the final stages of blackberry cv. Brazos micropropagation, including rooting and acclimatization. The experiments were carried out in the Plant Micropropagation Laboratory at UFPR, Curitiba-PR, during March 2000 until July 2001. In the in vitro rooting with or without immersion in IBA, the percentage of rooting was higher than 95% in both treatments. In the experiment of ex vitro rooting, with micro-shoots proceeding from the multiplication with different cytokinins, the rooting and survival rates were of 100%. In the final stage it was tested the influence of sucrose of the in vitro rooting media on the acclimatization. All the treatments showed 100% of survival. It can be concluded that an efficient in vitro rooting can be realized without both IBA and sucrose in the culture media with acclimatization under plastic tunnel or ex vitro rooting and acclimatization

  18. El alba-tcp mirado con buenos ojos

    Directory of Open Access Journals (Sweden)

    Christopher David Absell

    2012-01-01

    Full Text Available El surgimiento de la Alianza Bolivariana para los Pueblos de Nuestra América-Tratado de Comercio de los Pueblos (alba-tcp representa una novedad de importancia regional e internacional; sin embargo hasta la fecha el trabajo académico sobre la organización ha sido escaso y se ha caracterizado por tener un enfoque metodológico descriptivo carente de investigación empírica comprensiva. En este artículo sostengo que este enfoque metodológico ha imbuido la literatura de un sesgo positivo a favor del alba-tcp y sus proyectos de desarrollo. El objetivo de este artículo es examinar esta literatura para determinar una agenda de investigación para el estudio del alba-tcp.

  19. Chemical Constituents and Biological Activities of Artemisia herba-alba

    Directory of Open Access Journals (Sweden)

    Abou El-Hamd H. Mohamed

    2010-01-01

    Full Text Available Artemisia, one of the larger genera in the family Asteraceae and the largest genus in the tribe Anthemideae, comprises from 200 to more than 500 taxa at the specific or subspecific level. Many Artemisia species have a high economic value in several fields, as food plants and as antihelminthic and antimalaria in medicine. Artemisia herba-alba was known for its therapeutic and medicinal properties, it was used in both traditional and modern medicine. Several papers have been published on the chemical composition of specimens of A. herba-alba. The aim of this work is to review all available scientific literature published on A. herba-alba. The focus will be on the chemical constitutions which have been identified from this species, in addition to all of the reported biological activites of this species have been included as well as the pharmacology and toxicology

  20. Antinociceptive properties of morusin, a prenylflavonoid isolated from Morus nigra root bark.

    Science.gov (United States)

    de Souza, M M; Bittar, M; Cechinel-Filho, V; Yunes, R A; Messana, I; Delle Monache, F; Ferrari, F

    2000-01-01

    The antinociceptive effects of morusin (1), the main prenylflavonoid present in the Morus nigra root barks have been investigated in classical models of pain in mice. The results showed that 1 exhibits a promising antinociceptive or analgesic profile by the intraperitoneal route, being more potent than some standard drugs used as reference. The mechanism by which the morusin exerts antinociceptive activity still remains undetermined, but our results strongly suggest that it involves the participation of the opioid system.

  1. Phytochemical profile and biological potential of mulberry teas (Morus nigra L.)

    OpenAIRE

    Čestić, Slađana; Radojković, Marija M.; Cvetanović, Aleksandra; Mašković, Pavle; Đurović, Saša

    2016-01-01

    Black mulberry is the most medicinally important plant of the genus Morus. This study examines the chemical composition, antioxidant, antimicrobial and cytotoxic activity of mulberry leaves tea. The tea drinks were prepared in water (70, 80, 90 ° C and boiling temperature). This study has shown that mulberry teas have high antioxidant, antimicrobial and cytotoxic activity. Experimental investigation has shown that the best conditions for preparing mulberry tea are with boiling water. Mulberry...

  2. Effect of Morus nigra aqueous extract treatment on the maternal-fetal outcome, oxidative stress status and lipid profile of streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Volpato, G T; Calderon, I M P; Sinzato, S; Campos, K E; Rudge, M V C; Damasceno, D C

    2011-12-08

    Morus nigra, commonly known as black mulberry, is widely used in Brazilian folk medicine for the diabetes treatment. To evaluate the effect of Morus nigra aqueous extract treatment on maternal lipid and oxidative stress profile, reproductive outcomes, and also fetal anomaly incidence from diabetic and non-diabetic rats. Diabetes was induced by streptozotocin (40 mg/kg) in virgin female Wistar rats. Morus nigra leaf aqueous extract (400 mg/kg) was administered from day 0 to 20 of pregnancy. At day 21 of pregnancy, all rats were anesthetized and killed to obtain blood samples and maternal-fetal data. After treatment with Morus nigra extract, non-diabetic and diabetic rats presented no glycemic changes. Fetuses from diabetic dams, regardless of Morus nigra treatment, were small for pregnancy age. In diabetic dams, plant treatment caused reduced MDA, cholesterol, triglycerides and VLDL levels, and decreased placental index and weight as compared to diabetic group. The fetuses from diabetic rats treated with Morus nigra extract had lower frequency of skeletal and visceral anomalies as compared to diabetic group. Thus, Morus nigra leaf aqueous extract failed to control hyperglycemia in diabetic rats. However, Morus nigra treatment had antioxidant effect, contributing to reduce incidence of internal anomalies in offspring from diabetic dams. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  3. Plant regulators for rooting of hardwood cuttings of blackberry plants cv. Xavante/ Reguladores vegetais no enraizamento de estacas lenhosas da amoreira-preta cv. Xavante

    Directory of Open Access Journals (Sweden)

    Renato Vasconcelos Botelho

    2008-08-01

    , foram avaliadas as seguintes variáveis: porcentagem de estacas enraizadas, comprimento médio das raízes, número de raízes, massa fresca de raízes, porcentagem de estacas com brotações e número de folhas. O tratamento de estacas lenhosas a 2000 mg L-1 AIB obteve a maior porcentagem de enraizamento (60%. No entanto, o tratamento a 1000 mg L-1 AIB já atingiu enraizamento elevado (56%, com 96% de estacas brotadas e 17,6 raízes por estaca. As Estacas tratadas com PBZ apresentaram maior presença de calo e não enraizaram não sendo possível recomendar este produto para o enraizamento da amoreira-preta cv. Xavante nas concentrações testadas.

  4. Microbiological characterization and functionality of set-type yogurt fermented with potential prebiotic substrates Cudrania tricuspidata and Morus alba L. leaf extracts.

    Science.gov (United States)

    Oh, Nam Su; Lee, Ji Young; Joung, Jae Yeon; Kim, Kyung Su; Shin, Yong Kook; Lee, Kwang-Won; Kim, Sae Hun; Oh, Sangnam; Kim, Younghoon

    2016-08-01

    The objective of this study was to investigate the effect of 2 plant leaf extracts on fermentation mechanisms and health-promoting activities and their potential as a nutraceutical prebiotics ingredient for application in dairy products. The individual active phenolic compounds in the plant extract-supplemented milk and yogurts were also identified. Compared with control fermentation, the plant extracts significantly increased the growth and acidification rate of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus. In particular, plant extract components, including monosaccharides, formic acid, and hydroxycinnamic acid, such as neo-chlorogenic, chlorogenic, and caffeic acid, together play a stimulatory role and cause this beneficial effect on the growth of yogurt culture bacteria through fermentation. In addition, supplementation with the plant extracts enhanced antioxidant activities with increased total phenolic contents, especially the highest antioxidant activity was observed in yogurt supplemented with Cudrania tricuspidata leaf extract. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  5. Inhibitory effects of constituents from Morus alba var. multicaulis on differentiation of 3T3-L1 cells and nitric oxide production in RAW264.7 cells

    National Research Council Canada - National Science Library

    Yang, Zhi-Gang; Matsuzaki, Keiichi; Takamatsu, Satoshi; Kitanaka, Susumu

    2011-01-01

    .... multicaulis Perro. (Moraceae). Their structures were determined by spectroscopic methods. The prenyl-flavonoids 11-14, 16, triterpenoids 17,18 and 20 showed significant inhibitory activity towards the differentiation of 3T3-L1 adipocytes...

  6. The Effects of Dietary Mulberry Leaves (Morus alba L. on Chicken Performance, Carcass, Egg Quality and Cholesterol Content of Meat and Egg

    Directory of Open Access Journals (Sweden)

    Paichok PANJA

    2013-04-01

    Full Text Available Experiment I (Broilers The experimental design was completely randomized. Two hundred and forty of 3 week old male broilers were divided into 5 groups with 4 replicates of 12 birds each. The protein and metabolizable energy content of basal diets were 20 % and 3,000 kcal/kg, respectively. The basal diets were supplemented with mulberry leaves at 0, 0.5, 1.0, 1.5 and 2.0 % of diet. The results demonstrated that feed intake, weight gain, feed efficiency, nitrogen and energy intake were not significantly different among treatments (P > 0.05. The carcass quality showed that carcass weight, dressing percentage and percent of abdominal fat pad were also not significantly different (P > 0.05. The blood cholesterol and triglyceride were significant (P < 0.05 lower at higher levels of mulberry leaves. However, the cholesterol content in thigh meat was not significantly different among treatments (P > 0.05.   Experiment II (Layers The experimental design was completely randomized and two hundred of 27 week old laying hens were divided into 5 groups with 4 replicates of 10 birds each. The protein and metabolizable energy content of basal diet were 16 % and 2,750 kcal/kg, respectively. The basal diets were supplemented with mulberry leaves at 0, 0.5, 1.0, 1.5 and 2.0 % of diet. The results found that feed intake, egg weight, egg mass, and egg quality were not significantly different among treatments (P > 0.05. The blood cholesterol was found to decrease (P < 0.05 at 0.5, 1.0 and 2.0 % of mulberry leaves inclusion. Likewise, triglyceride was also found to decrease (P < 0.05 at 0.5 to 1.5 % of inclusion. In addition, yolk cholesterol content was found to decrease and was significantly different (P < 0.05 at 2 % of inclusion.

  7. Efecto de los niveles de fertilización, la variedad y la época en los contenidos de saponinas esteroidales en morera(Morus alba L.

    Directory of Open Access Journals (Sweden)

    D. E. García

    2005-01-01

    Full Text Available Con el objetivo de determinar el efecto de la fertilización orgánica (0, 100, 300 y 500 kg N/ha/año, la variedad (Cubana, Indonesia, Tigreada y Acorazonada y la época (periodo lluvioso y poco lluvioso en el contenido total de saponinas esteroidales (SE de la fracción comestible de la morera, se llevó a cabo una investigación con un diseño de bloques al azar con arreglo factorial 4 x 4 x 2 (n=5. No se observaron interacciones significativas de ningún orden entre los factores en estudio (P<0,05 y, tanto en las hojas como en los tallos tiernos, los niveles de abonado no afectaron significativamente las concentraciones. La variedad Acorazonada presentó el máximo contenido de SE en las hojas (11,57a mg/gMS, mientras que la Indonesia, Cubana y Tigreada mostraron niveles más bajos (11,00ab; 10,65b y 9,30c mg/gMS, respectivamente. En los tallos tiernos no se observaron diferencias significativas entre las variedades. La mayor concentración de SE (en las hojas se observó en el periodo lluvioso (14,38 mg/gMS, mientras que en los tallos tiernos el nivel más elevado correspondió con el periodo poco lluvioso (2,52 mg/gMS. Los resultados permiten concluir que, entre los factores evaluados en las hojas, la época y la variedad, mostraron la mayor importancia. En los tallos tiernos sólo la época ocasionó variaciones marcadas. En ambas partes de la planta, los niveles de fertilización no produjeron efecto en las concentraciones de las SE.

  8. Astragalin, a Flavonoid from Morus alba (Mulberry Increases Endogenous Estrogen and Progesterone by Inhibiting Ovarian Granulosa Cell Apoptosis in an Aged Rat Model of Menopause

    Directory of Open Access Journals (Sweden)

    Min Wei

    2016-05-01

    Full Text Available Background: To determine the mechanism by which the flavonoid glycoside astragalin (AST reduces ovarian failure in an aged rat model of menopause. Methods: The in vivo effect of AST on granulosa cell (GC apoptosis in aged female rats was determined using flow cytometry. In vitro, the effects of AST on cultured GCs were investigated using the MTT proliferation assay and western blot assays. Results: Aged rats had significantly higher GC apoptosis as compared with young female rats. Treatment of aged rats with AST (all three doses; p < 0.01 or Progynova (p < 0.01 significantly reduced GC apoptosis as compared with the aged controls. The proportions of total apoptotic GCs was 25.70%, 86.65%, 47.04%, 27.02%, 42.09% and 56.42% in the normal, aged, 17β-estradiol (E2, high dose AST, medium dose AST, and low dose AST-treated groups, respectively. Significant increases of serum E2 and P4 levels, as well as altered levels of serum follicle stimulating hormone (FSH and luteinizing hormone (LH levels. In cultured rat GCs, AST stimulated GC proliferation, E2 and progesterone (P4 secretion, reduced apoptosis, reduced the level of the pro-apoptotic protein Bcl-2 (p < 0.01, but had no effect on BAX. Conclusions: AST enhanced ovarian function in aged female rats by increasing E2 and P4 levels, and reducing ovarian GC apoptosis via a mechanism involving Bcl-2. These data demonstrate a new pharmacological activity for AST, as well as a novel mechanism of action, and further suggest that AST may be a new therapeutic agent for the management of menopausal symptoms.

  9. The use of in vitro gas production technique to evaluate molasses supplementation to mulberry (morus alba and rice straw mixed diets

    Directory of Open Access Journals (Sweden)

    Dwi Yulistiani

    2007-12-01

    Full Text Available Mulberry foliages have high nutritive value (protein content, digestibility and degradability, therefore it is potential to be used as a supplement to poor quality roughages. The objective of this experiment was to evaluate the effect of addition of fermentable energy in the mixed of mulberry and rice straw basal diet. A control diet consisted of either rice straw (RS or urea treated rice straw mixed with mulberry foliage (URS with ratio of 60 : 40%. Treatment was formulated by supplementation of control diet with molasses (as sources of fermentable energy at 3 levels (5, 10 and 15%. The study was conducted in a 2 x 4 factorial experiment, consisted of 2 levels rice straw (untreated and urea treated and 4 levels molasses supplementation (control and 3 levels for molasses. Diets were evaluated using in vitro gas production. The fermentation kinetics was determined from the incubation of 200 mg sample during 96 hours. The calculation of the kinetics based on exponential equation P = A+ B (1-e-ct. A shorter gas production test was carried out to determine truly degradable fermented substrates (in vitro true organic matter degradability/IVTOMD by incubating 500 mg of samples 24 hours. The result showed that there was no significant interaction between rice straw treatment and molasses supplementation on fermentation characteristics, in vitro true dry matter digestibility, fermented substrate and total volatile fatty acid (VFA production. However there was a significant interaction between rice straw treatment on partitioning factor (PF, gas produced, propionic acid production and ratio between acetic acid and propionic acid. Molasses supplementation significantly (P<0.05 decreased gas production and ratio of acetic to propionic acid, and increase PF, propionic acid production in untreated rice straw mulberry (RSM basal diet. It is concluded that molasses supplementation to RSM diet decreased gas production and ratio of C2/C3, and increased PF and propionic acid production.

  10. Analgesic and anti-Inflammatory effect of UP3005, a botanical composition Containing two standardized extracts of Uncaria gambir and Morus alba

    OpenAIRE

    Mesfin Yimam; Young-Chul Lee; Tae-Woo Kim; Breanna Moore; Ping Jiao; Mei Hong; Hyun-Jin Kim; Jeong-Bum Nam; Mi-Ran Kim; Jin-Sun Oh; Sabrina Cleveland; Eu-Jin Hyun; Min Chu; Qi Jia

    2015-01-01

    Background: Osteoarthritis (OA) is a chronic debilitating degenerative joint disease characterized by cartilage degradation and synovial inflammation exhibited by clinical symptoms such as joint swelling, synovitis, and inflammatory pain. Present day pain relief therapeutics heavily relies on the use of prescription and over the counter nonsteroidal anti-inflammatory drugs as the first line of defense where their long-term usage causes detrimental gastrointestinal and cardiovascular-related s...

  11. Características físicas y valor nutritivo del ensilaje de morera (morus alba mezclado con forraje de maíz

    Directory of Open Access Journals (Sweden)

    Carlos Boschini

    2003-01-01

    Full Text Available Follaje de morera y maíz cosechados a 120 y 135 días respectivamente fueron ensilados, picados y mezclados en proporciones porcentuales de 100:0, 75:25, 50:50, 25:75 y 0:100 en base a la masa seca. La concentración de carbohidratos solubles fue uniformada a 10% en todos los tratamientos con la adición de melaza en proporción a las mezclas experimentales. El periodo de fermentación fue de 90 días. El forraje de morera (proporción 100:0 compactado tuvo una densidad de 693 kg/m3 y el forraje de maíz (proporción 0:100 fue de 541 kg/m3, observándose marcadas diferencias iniciales (P≤0,01 entre las mezclas forrajeras. Al final del periodo experimental, el ensilado mostró densidades entre 750 y 556 kg/m3, con valores intermedios (P≤0,01 al disminuir la proporción de morera en la mezcla. Se detectaron pérdidas de materia seca entre los tratamientos (P≤0,01, entre 6,8 kg/m3 y 18,3 kg/m3. La evaluación organoléptica tuvo una calificación de muy bueno a excelente en las mezclas 75:50, 50:50, 25:75 y 0:100, no así en el ensilaje de solo morera (100:0, el cual presentó un ligero olor amoniacal. La concentración de materia seca en el material fresco fue superior al encontrado en el material ensilado (P≤0,05. La concentración de proteína cruda entre las mezclas se observó claramente diferenciada (P≤0,01, tanto al inicio como al final de periodo de fermentaci ón. Similar respuesta se observó en la concentración de fibra neutro detergente y de las cenizas totales.

  12. USOS DE LA MORERA (Morus alba EN LA ALIMENTACIÓN DEL CONEJO. EL ROL DE LA FIBRA Y LA PROTEÍNA EN EL TRACTO DIGESTIVO

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    David Mora-Valverde

    2010-01-01

    Full Text Available El objetivo de este trabajo fue recopilar las principales características de la digestión del conejo, enfocándose en la digestibilidad de la fibra y la proteína de la dieta para valorar el potencial desempeño de un material con características como la morera. El sistema selectivo de fibra con el que cuenta este lagomorfo, le otorga una ventaja alimenticia especializada ya que excreta de manera eficiente los materiales de baja calidad. La composición bromatológica de la morera y su elevada digestibilidad la hacen muy promisoria para su incorporación en dietas de conejos, exigiendo la evaluación de ésta desde diversos niveles de inclusión, los cuales permitan definir márgenes de sostenibilidad que mejoren su uso en conejos. Se presenta información sobre el empleo de esta fuente en dietas para conejos.

  13. Onion and weed response to mustard (Sinapis alba) seed meal

    Science.gov (United States)

    Weed control in organic onion production is often difficult and expensive, requiring numerous cultivations and extensive hand-weeding. Onion safety and weed control with mustard seed meal (MSM) derived from Sinapis alba was evaluated in greenhouse and field trials. MSM applied at 110, 220, and 440 g...

  14. Chemical composition and antigenotoxic properties of Lippia alba essential oils

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    Molkary Andrea López

    2011-01-01

    Full Text Available The present work evaluated the chemical composition and the DNA protective effect of the essential oils (EOs from Lippia alba against bleomycin-induced genotoxicity. EO constituents were determined by Gas Chromatography/Mass Spectrometric (GC-MS analysis. The major compounds encountered being citral (33% geranial and 25% neral, geraniol (7% and trans-β-caryophyllene (7% for L. alba specimen COL512077, and carvone (38%, limonene (33% and bicyclosesquiphellandrene (8% for the other, COL512078. The genotoxicity and antigenotoxicity of EO and the compounds citral, carvone and limonene, were assayed using the SOS Chromotest in Escherichia coli. The EOs were not genotoxic in the SOS chromotest, but one of the major compound (limonene showed genotoxicity at doses between 97 and 1549 mM. Both EOs protected bacterial cells against bleomycin-induced genotoxicity. Antigenotoxicity in the two L. alba chemotypes was related to the major compounds, citral and carvone, respectively. The results were discussed in relation to the chemopreventive potential of L. alba EOs and its major compounds.

  15. Chemical composition and antigenotoxic properties of Lippia alba essential oils.

    Science.gov (United States)

    López, Molkary Andrea; Stashenko, Elena E; Fuentes, Jorge Luis

    2011-07-01

    The present work evaluated the chemical composition and the DNA protective effect of the essential oils (EOs) from Lippia alba against bleomycin-induced genotoxicity. EO constituents were determined by Gas Chromatography/Mass Spectrometric (GC-MS) analysis. The major compounds encountered being citral (33% geranial and 25% neral), geraniol (7%) and trans-β-caryophyllene (7%) for L. alba specimen COL512077, and carvone (38%), limonene (33%) and bicyclosesquiphellandrene (8%) for the other, COL512078. The genotoxicity and antigenotoxicity of EO and the compounds citral, carvone and limonene, were assayed using the SOS Chromotest in Escherichia coli. The EOs were not genotoxic in the SOS chromotest, but one of the major compound (limonene) showed genotoxicity at doses between 97 and 1549 mM. Both EOs protected bacterial cells against bleomycin-induced genotoxicity. Antigenotoxicity in the two L. alba chemotypes was related to the major compounds, citral and carvone, respectively. The results were discussed in relation to the chemopreventive potential of L. alba EOs and its major compounds.

  16. Revisión taxonómica del complejo Centaurea alba L. (Asteraceae en la Península Ibérica

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    Devesa, J. A.

    2011-12-01

    Full Text Available Taxonomic revision of the Centaurea alba L. complex (Asteraceae in the Iberian Peninsula.- A taxonomic revision of the Centaurea alba L. complex (Centaurea L. sect. Centaurea in the Iberian Peninsula is presented, which is represented by two species, C. alba and C. costae Willk. Three subspecies of C. alba with reasonably well-defined areas are recognized: C. alba subsp. alba, with three varieties –alba, macrocephala Pau and latronum (Pau E. López & Devesa-, C. alba subsp. aristifera (Pau E. López & Devesa and C. alba subsp. tartesiana Talavera, two of them are proposed as new nomenclatural combinations. Centaurea costae is segregated from C. alba and three varieties are recognized: costae, montsicciana Pau & Font Quer and maluqueri Font Quer. In the case of C. alba var. latronum, C. alba var. macrocephala and C. alba subsp. aristifera, the chromosome number was also studied (2n = 18.Revisión taxonómica del complejo Centaurea alba L. (Asteraceae en la Península Ibérica.- Se efectúa la revisión taxonómica del complejo de Centaurea alba L. (Centaurea L. sect. Centaurea en la Península Ibérica, representado por dos especies, C. alba y C. costae Willk. Para C. alba se reconocen tres subespecies con áreas bien definidas: C. alba subsp. alba, con tres variedades –alba, macrocephala Pau y latronum (Pau E. López & Devesa-, C. alba subsp. aristifera (Pau E. López & Devesa y C. alba subsp. tartesiana Talavera, proponiéndose dos nuevas combinaciones. Se segrega Centaurea costae de C. alba, y se reconocen para ella tres variedades: costae, montsicciana Pau & Font Quer y maluqueri Font Quer. En el caso de C. alba var. latronum, C. alba var. macrocephala y C. alba subsp. aristifera se ha estudiado además el número cromosómico (2n = 18.

  17. Meio de cultura, concentração de AIB e tempo de cultivo no enraizamento in vitro de amoreira-preta e framboeseira Culture environment, IBA concentration and cultivation time on in vitro rooting of blackberry and raspberry

    Directory of Open Access Journals (Sweden)

    Luciane Nolasco Leitzke

    2009-06-01

    Full Text Available A propagação da amoreira-preta e da framboeseira dá-se principalmente por meio de estacas de raiz e mesmo de hastes novas, contudo, já é crescente o interesse pelo uso da micropropagação como um método alternativo de propagação . O enraizamento é uma das etapas mais difícieis, onde a definição do meio de cultivo, da concentração ótima de AIB para o enraizamento, constitui um passo importante, por isso objetivou-se com este experimento determinar o melhor meio de cultivo, melhor tipo de cultivo e a melhor concentração de AIB no meio de cultura para o enraizamento in vitro da amoreira-preta 'Xavante' e de framboeseira 'Batum' e 'Heritage'. O material vegetal utilizado foram microestacas apicais com duas folhas, com cerca de 1 cm de comprimento, oriundas do cultivo in vitro. Os fatores estudados foram o tipo de meio de cultura MS e WPM - Wood Plant Media, a concentração de AIB no meio de cultura e o tempo de cultivo das microestacas em meio com AIB. O meio WPM, em concentrações baixas, menores de 3 µM de AIB, induziram maiores médias de enraizamento e comprimento. Concentrações altas de AIB induziram a formação de calo, para amoreira-preta, 'Xavante'. Para a framboeseira o meio WPM, com menores concentrações de AIB (0 e 3 µM, mostrou as melhores médias no número de raízes, comprimento de raízes e pequena intensidade de calo; com as maiores concentrações de AIB, ocorreu maior aparecimento de calo.The propagation of blackberry and raspberry occur mainly through root cutting and even of softwoods stem; however, it is already increasing the interest for the use of the micropropagation as an alternative technique of vegetative propagation of fruit plants, since the rooting is one of the most difficult stages, in which the definition of both the culture environment and the best concentration of AIB for the rooting, is an important stage. For that purpose, the aim of this research was to determine the best cultivation

  18. Uso de reguladores de crescimento para a superação da dormência e sua influência na brotação, no florescimento e na produção da amoreira-preta

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    Daniela Mota Segantini

    2011-10-01

    Full Text Available O presente trabalho foi conduzido na Fazenda Experimental São Manuel, da Faculdade de Ciências Agronômicas da UNESP, localizada no município de São Manuel-SP. Foram avaliadas amoreiras-pretas da cultivar Tupy com 1 ano de idade, conduzidas em quatro hastes, no sistema de espaldeira em T, com 1,2 metro de altura. O espaçamento utilizado foi de 0,6 m entre plantas x 4,0 metros entre linhas e a densidade de plantio de 4.166 plantas.ha-1. O delineamento experimental adotado foi o de blocos ao acaso, com 4 repetições, 15 tratamentos, e a parcela experimental composta por duas plantas, rodeadas por bordadura. Em 27 de julho de 2010, efetuaram-se a poda de produção e a quebra de dormência das plantas. Para tanto, foram testadas as seguintes doses de Dormex® (49% de cianamida hidrogenada: T1=0,0%; T2=1,5%,; T3=3,0%; T4=4,5%, e T5=6,0%, de Bioalho® (70% de extrato de alho: T6=0,0%; T7=2,0%; T8=4,0%; T9=6,0%, e T10=8,0% e de Stimulate® (0,005% de auxina, 0,005% de giberelina e 0,009% de citocinina: T11=0,0%; T12=2,0%; T13=4,0%; T14=6,0%, e T15=8,0% . Avaliaram-se a fenologia e a produtividade da amoreira-preta 'Tupy' em função dos reguladores e das doses utilizadas. A quebra de dormência efetuada com cianamida hidrogenada, nas doses de 3,0, 4,5% e 6,0%, antecipou e uniformizou a brotação e florescimento, além proporcionar os maiores valores de porcentagem de brotação e produtividade, sendo estas as melhores doses do regulador vegetal para cultura cultivada na região. Os tratamentos em que se utilizaram extrato de alho e Stimulate® apresentaram dados de brotação e produtividade muito inferiores, quando comparados aos apresentados pelos tratamentos em que se aplicou cianamida hidrogenada. Assim, fazem-se necessários novos estudos em busca de reguladores vegetais alternativos que sejam tão eficazes quanto à cianamida hidrogenada.

  19. Environ: E00366 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00366 Morus leaf Crude drug Morus alba [TAX:3498], Morus bombycis [TAX:66393] Mora...ceae (mulberry family) Morus leaf or small branch with leaf Crude drugs [BR:br08305] Dicot plants: rosids Moraceae (mulberry family) E00366 Morus leaf ...

  20. Acute hypotensive and diuretic activities of Artemisia herba alba aqueous extract in normal rats

    Institute of Scientific and Technical Information of China (English)

    Naoufel Ali Zeggwagh; Jean Baptiste Michel; Mohamed Eddouks

    2014-01-01

    Objective:To evaluate the effect of Artemisia herba alba (A. herba alba) intravenous injection on cardiovascular and renal function in normal rats. Methods:The effect of intravenous injection of A. herba alba extract at the different doses of 50, 100 and 200 mg/kg was investigated in normal rats. Diuresis, heart frequency and electrolytes concentrations were analyzed. Results: Intravenous bolus injection of A. herba alba at the different doses of 50, 100 and 200 mg/kg produced a dose dependent reduction in arterial blood pressure (P Conclusions: We conclude that the aqueous A. herba alba extract possesses a potent acute hypotensive effect on normal rats. In addition, A. herba alba perfusion may affect renal function to increase urine and electrolytes excretion.

  1. Comparison Between in Vivo and in Vitro Metabolite Production of Morus nigra.

    Science.gov (United States)

    Ferrari, F; Monacelli, B; Messana, I

    1999-02-01

    Nine flavonoids and seven Diels-Alder type adducts were isolated from the acetonic extract of MORUS NIGRA I. root bark, and three flavonoids and six Diels-Alder type adducts were isolated from the acetonic extract of M. NIGRA L. callus. However, none of the metabolites isolated from the root bark was found in the cell culture. Moreover, adducts isolated from the plant and cell culture showed not only a different cyclohexene ring substitution but also a different stereochemistry, that was found CIS-TRONS in the metabolites isolated from callus, and TRANS-TRANS in those isolated from the root bark.

  2. EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF LEAF TEAS BLACKBERRY (MORUS NIGRA)

    OpenAIRE

    P. Zanco; de Souza, E F; Bonacorsi,C.

    2017-01-01

    Brazil has a long tradition in the use of plants, and is the same used in different ways, such as in the form of infusions and decoctions, for therapeutic purposes. Morus nigra species is a plant that has been used in different parts of the world as phytotherapy. This plant is known as mulberry, black, blackberry black or blackberry and various parts of the plant, such as leaves, fruit, bark and roots are used by the population, demonstrating anti-inflammatory activity, antioxidant, hypoglyce...

  3. Phytochemical profile and biological potential of mulberry teas (Morus nigra L.

    Directory of Open Access Journals (Sweden)

    Čestić Slađana

    2016-01-01

    Full Text Available Black mulberry is the most medicinally important plant of the genus Morus. This study examines the chemical composition, antioxidant, antimicrobial and cytotoxic activity of mulberry leaves tea. The tea drinks were prepared in water (70, 80, 90 ° C and boiling temperature. This study has shown that mulberry teas have high antioxidant, antimicrobial and cytotoxic activity. Experimental investigation has shown that the best conditions for preparing mulberry tea are with boiling water. Mulberry teas could be regarded as a promising source of bioactive natural compounds, which can be used both as a food supplement and herbal remedy.

  4. Regionalism and South American orientation: UNASUR and ALBA

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    Rut Diamint

    2013-04-01

    Full Text Available This study examines whether or not integration in South America is shaping a new type of regionalism. The author takes the cases of UNASUR and ALBA and shows – on the basis of the characteristics of institutionality, leaderships and identities – what the incentives for cooperation are. One of the essential elements for all integration is the defence agreements that are articulated in different ways in each of the organisations. The article attempts to show that both UNASUR and ALBA were structured using a new logic, by prioritising political agreements over commercial ones. The author describes a new South American notion that is still under construction, but which is more narrative- and discourse-oriented than involving formalised actions. As a result, we cannot conclude that this represents a new type of regionalism.

  5. Tissue immunostaining for factor XIIIa in dermal dendrocytes of pityriasis alba skin lesions

    OpenAIRE

    Carneiro, Francisca Regina Oliveira; Amaral,Gabriela Borborema do; Mendes,Maiana Darwich; Quaresma,Juarez Antônio Simões

    2014-01-01

    BACKGROUND: Pityriasis alba affects 1% of the world population and about 9.9% of the children in Brazil. However, its etiology remains uncertain. OBJECTIVE: The objective of the present study was to evaluate the immunoexpression of factor XIIIa in dermal dendrocytes of skin lesions of pityriasis alba. METHOD: Twenty patients with pityriasis alba and 20 patients with atopic dermatitis underwent biopsy. The dermal dendrocytes marked by factor XIIIa were counted by means of immunohistochemic...

  6. Flavonoids from the flowers of Nymphaea alba L.

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    Jerzy Jambor

    2014-01-01

    Full Text Available Ten flavonoids were obtained from the flowers of Nymphaea alba L. Their structures were determined mainly on the basis of spectral analyses (UV, 'H NMR, MS. The following aglycons were isolated: quercetin, kaempferol, isokaempferide and apigenin as well as the following glycosides: quercetion 4'-β-xyloside, 3-methylquercetin 3'-β-xyloside and a mixture of quercetin 3-galactoside and 3-glucoside. The structures of three compounds obtained in very small amounts were determined in part.

  7. Phenolic arids from the flowers of Nymphaea alba L.

    Directory of Open Access Journals (Sweden)

    Jerzy Jambor

    2014-01-01

    Full Text Available Nympheae alba flowers contain a variety of phenolic acids. Crystalline ellagic and gallic acids were isolated as well as their methyl and ethyl esters. Their identification was made on the basis of the results of spectral analyses 1H NMR, 13C NMR, MS, UV. Small amounts of p-hydroxybenzoic, p-cumaric, vanillic and ferulic acids were isolated. The presence of bound forms of acids in the material was demonstrated.

  8. In vitro antimicrobial activity of mangrove plant Sonneratia alba

    Institute of Scientific and Technical Information of China (English)

    Shahbudin Saad; Muhammad Taher; Deny Susanti; Haitham Qaralleh; Anis Fadhlina Izyani Bt Awang

    2012-01-01

    Objective:To investigate the antimicrobial property of mangrove plant Sonneratia alba (S. alba). Methods: The antimicrobial activity was evaluated using disc diffusion and microdilution methods against six microorganisms. Soxhlet apparatus was used for extraction with a series of solvents, n-hexane, ethyl acetate and methanol in sequence of increasing polarity. Results:Methanol extract appeared to be the most effective extract while n-hexane extract showed no activity. The antimicrobial activities were observed against the gram positive bacteria Staphylococcus aureus (S. aureus) and Bacillus cereus (B. cereus), the gram negative Escherichia coli (E. coli) and the yeast Cryptococcus neoformans. Pseudomonas aeruginosa and Candida albicans appeared to be not sensitive to the concentrations tested since no inhibition zone was observed. E. coli (17.5 mm) appeared to be the most sensitive strain followed by S. aureus (12.5 mm) and B. cereus (12.5 mm). Conclusions:From this study, it can be concluded that S. alba exhibits antimicrobial activities against certain microorganisms.

  9. A New Diels-Alder Type Adduct and a New Flavone from the Stem and Root Bark of Morus mongolica

    Institute of Scientific and Technical Information of China (English)

    Jie KANG; Ruo Yun CHEN; De Quan YU

    2005-01-01

    A new Diels-Alder type adduct mongolicin G (1) and a new flavone 5'-(1", 1"-dimethylallyl)-5,7,2',4'-tetrahydroxyflavone (2) were isolated from the stem and root bark of Morus mongolica. Their structures were determined by spectroscopic analysis and chiroptical methods.

  10. Calydorea alba (Iridaceae: Tigridieae, a new species from Uruguay Calydorea alba (Iridaceae: Tigridieae, una nueva especie de Uruguay

    Directory of Open Access Journals (Sweden)

    G. G. Roitman

    2005-12-01

    Full Text Available Calydorea alba is described from wet grasslands of northern Uruguay. The new species resembles Calydorea azurea, but can be easily distinguished by the smaller white flowers (3.2-3.6 cm wide vs. 4.4-5.3 cm wide, and linear subulate not truncate, style branches.Se describe Calydorea alba proveniente de pastizales húmedos del norte de Uruguay. Esta nueva especie se asemeja a Calydorea azurea, pero puede ser fácilmente distinguida por sus flores blancas más pequeñas (3,2-3,6 cm vs. 4.4-5.3 cm, de diámetro, y las ramas de estilo lineares, subuladas no truncadas.

  11. Colorability of Wood Material with Punica granatum and Morus nigra Extracts

    Directory of Open Access Journals (Sweden)

    Ertan Özen

    2014-04-01

    Full Text Available The aim of this study was the development of an eco-friendly dye that may be used in furniture, woodworking, and upper surface treatment, with no ill effects to human health. The plant dyestuff was extracted from pomegranate skin (Punica granatum and black mulberry (Morus nigra using an ultrasonic method at different rates. The extraction percentage ratios were, by weight in distilled water, 25%, 30%, 35%, 40%, 45%, and 50% and were applied to Scotch pine (Pinus sylvestris L. and Oriental beech (Fagus orientalis L. through an immersion method. After that, the determination of color change under the accelerated weathering conditions and the feasibility as the upper surface stain of this dyestuff were studied. The values of total color change of the natural dye samples that were applied to the test material were determined after accelerated weathering processes lasting 50, 100, and 150 h, according to ISO 2470. The results showed the best color stability in Oriental beech wood that was treated with 30% pomegranate skin or black mulberry extract as well as Scotch pine wood that was treated with 25% pomegranate skin extract or 50% black mulberry extract. Exactly the same ΔE* values were obtained with the same dyestuff and same wood type, indicating close color stability behaviors. According to the results, both pomegranate skin (Punica granatum and black mulberry (Morus nigra can be used to color wood materials.

  12. EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF LEAF TEAS BLACKBERRY (MORUS NIGRA

    Directory of Open Access Journals (Sweden)

    P. Zanco

    2017-02-01

    Full Text Available Brazil has a long tradition in the use of plants, and is the same used in different ways, such as in the form of infusions and decoctions, for therapeutic purposes. Morus nigra species is a plant that has been used in different parts of the world as phytotherapy. This plant is known as mulberry, black, blackberry black or blackberry and various parts of the plant, such as leaves, fruit, bark and roots are used by the population, demonstrating anti-inflammatory activity, antioxidant, hypoglycemic, antimicrobial, among others. Because of the interest in research on the bioactivity of plant products and use of blackberry tea in the population, present study evaluated the antibacterial activity of teas M. nigra leaves obtained by infusion and decoction against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium, as well as the antioxidant potential of preparations by DPPH technique. Results revealed that the infusions and decoctions prepared with Morus nigra leaves showed no ability to inhibit the growth of bacteria tested. However, all preparations (decoctions and infusions obtained from the leaves showed antioxidant potential, demonstrated ability to reduce DPPH radical. Despite infusions and decoctions evaluated in this study do not show antimicrobial activity, both had significant antioxidant property. Considering the use of different plants, including mulberry tree, by the people, it is considered always important proof of their biological activities.

  13. Morus nigra plant leaves as biomonitor for elemental air pollution monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Daud, M.; Khalid, N.; Waheed, S.; Wasim, M.; Arif, M.; Zaidi, J.H. [Pakistan Institute of Nuclear Science and Technology, Islamabad (Pakistan). Chemistry Div.

    2011-07-01

    The present paper deals with the determination of 36 elements in 120 leaf samples of Morus nigra plant to assess their potential as biomonitor for elemental air pollution monitoring. The elemental quantification was made by employing Instrumental Neutron Activation Analysis (INAA) and Atomic Absorption Spectrometric (AAS) techniques. The leaf samples were collected in spring, summer and winter seasons from various sites in Islamabad with different types of anthropogenic activities as well as from a reference site with minimum of such activities. Twenty four soil samples from the respective sites were also analyzed. The reliability of the adopted procedures was established by analyzing the certified reference materials, i.e., citrus leaves-1572 and soil-7, from NIST and IAEA, respectively, under identical experimental conditions and comparing the results obtained with the certified values which are in quite good agreement with each other. The enrichment values and Pollution Load Index (PLI) of the determined elements were computed and discussed accordingly. The elemental translocation from soil to roots, stem and leaves has also been studied by analyzing these parts of the same plant. The results indicated that the leaves of Morus nigra plant have promising potential to monitor the extent of air pollution in the vicinity of industrial as well as in high traffic areas. (orig.)

  14. Brazilian Morus nigra Attenuated Hyperglycemia, Dyslipidemia, and Prooxidant Status in Alloxan-Induced Diabetic Rats.

    Science.gov (United States)

    Júnior, Ivanildo I da S; Barbosa, Humberto de Moura; Carvalho, Débora C R; Barros, Ruideglan de Alencar; Albuquerque, Flávia Peixoto; da Silva, Dionísio Henrique Amaral; Souza, Grasielly R; Souza, Nathália A C; Rolim, Larissa A; Silva, Flaviane M M; Duarte, Glória I B P; Almeida, Jackson R G da S; de Oliveira Júnior, Flávio Monteiro; Gomes, Dayane A; Lira, Eduardo C

    2017-01-01

    Morus nigra has been used popularly for several proposes, including diabetic. In an attempt to support medicinal value, the acute hypoglycemic, hypolipidemic, and antioxidant effects of the ethanolic extract of Morus nigra (EEMn 200 or 400 mg/kg b.w.) were evaluated in normal and alloxan-induced diabetic treated for 14 days. Serum biochemical and antioxidant analysis were performed at the end of experiment. Oral glucose tolerance test was performed at 10th and 15th days. Chromatographic analysis by HPLC-DAD of EEMn was performed. Insulin was used as positive control to glycemic metabolism as well as fenofibrate to lipid metabolism. EEMn (400 mg/kg/day) reduced fasting and postprandial glycaemia, improved oral glucose tolerance, and reduced lipolysis and proteolysis in diabetic rats. EEMn decreased the blood levels of total cholesterol and increased HDL level when compared to the diabetic control rats. At higher levels, EEMn reduced triglycerides and VLDL levels in diabetic rats. Also, EEMn reduced malondialdehyde and increased the reduced glutathione levels in liver of diabetic rats. Chromatographic analysis identified the presence of the flavonoids rutin, isoquercetin, and kaempferitrin. Acute EEMn treatment reduced hyperglycemia, improved oral glucose tolerance, and minimized dyslipidemia and oxidative stress leading to a reduction in atherogenic index in alloxan-induced diabetic rats.

  15. Brazilian Morus nigra Attenuated Hyperglycemia, Dyslipidemia, and Prooxidant Status in Alloxan-Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Ivanildo I. da S. Júnior

    2017-01-01

    Full Text Available Morus nigra has been used popularly for several proposes, including diabetic. In an attempt to support medicinal value, the acute hypoglycemic, hypolipidemic, and antioxidant effects of the ethanolic extract of Morus nigra (EEMn 200 or 400 mg/kg b.w. were evaluated in normal and alloxan-induced diabetic treated for 14 days. Serum biochemical and antioxidant analysis were performed at the end of experiment. Oral glucose tolerance test was performed at 10th and 15th days. Chromatographic analysis by HPLC-DAD of EEMn was performed. Insulin was used as positive control to glycemic metabolism as well as fenofibrate to lipid metabolism. EEMn (400 mg/kg/day reduced fasting and postprandial glycaemia, improved oral glucose tolerance, and reduced lipolysis and proteolysis in diabetic rats. EEMn decreased the blood levels of total cholesterol and increased HDL level when compared to the diabetic control rats. At higher levels, EEMn reduced triglycerides and VLDL levels in diabetic rats. Also, EEMn reduced malondialdehyde and increased the reduced glutathione levels in liver of diabetic rats. Chromatographic analysis identified the presence of the flavonoids rutin, isoquercetin, and kaempferitrin. Acute EEMn treatment reduced hyperglycemia, improved oral glucose tolerance, and minimized dyslipidemia and oxidative stress leading to a reduction in atherogenic index in alloxan-induced diabetic rats.

  16. EFEITO ALELOPÁTICO E MOLUSCICIDA DE AMORA (Morus rubra L.

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    ANA CRISTINA MENDES MIRANDA

    2012-01-01

    Full Text Available Secondary metabolite produced in some plants can provoke alterations in the development of other plants or even other organisms. The aim of this paper was to identify possible allelopathic effects and biologic control of Achatina fulica Bowdich with aqueous extracts of Morus rubra L. leaves. For obtaining the aqueous extract, we used previously dried leaves in concentration of 1g 10 mL-1. Five concentrations of each aqueous extract were tested (10, 30, 50, 70 and 100% and compared to control (0.0%, distilled water. The aqueous extracts of Morus rubra showed up allelopathic potentialities on the seed germination and in the growth of the shoot and the root system of the tested species, considering that the reduction in the germination and initial growth increased with the rising of the aqueous extracts concentrations used and, in the biologic control, only in the final mass of A. fulica. The results indicated the existence of allelopathic effect, but not molluscicide of M. rubra.

  17. Inhibition of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase (Ex Vivo by Morus indica (Mulberry

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    Vanitha Reddy Palvai

    2014-01-01

    Full Text Available Phytochemicals are the bioactive components that contribute to the prevention of cardiovascular and other degenerative diseases. Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA reductase would be an effective means of lowering plasma cholesterol in humans. The present study explores the HMG CoA reductase inhibitory effect of extracts from leaves of Morus indica varieties, M5, V1, and S36, compared with the statin, using an ex vivo method. The assay is based on the stoichiometric formation of coenzyme A during the reduction of microsomal HMG CoA to mevalonate. Dechlorophyllised extract of three varieties was studied at 300 µg. The coenzyme A released at the end of assay in control (100.31 nmoles and statins (94.46 nm was higher than the dechlorphyllised extracts of the samples. The coenzyme A released during the reduction of HMG CoA to mevalonate in dechlorophyllised extracts of the samples was as follows: S36 < M5 < V1. The results indicated that the samples were highly effective in inhibiting the enzyme compared to statins (standard drug. The results indicate the role of Morus varieties extracts in modulating the cholesterol metabolism by inhibiting the activity of HMG CoA reductase. These results provide scope for designing in vivo animal studies to confirm their effect.

  18. Counter-Hegemonic Regionalism and Higher Education for All: Venezuela and the ALBA

    Science.gov (United States)

    Muhr, Thomas

    2010-01-01

    This paper employs new regionalism theory and regulatory regionalism theory in its analysis and theorisation of the Bolivarian Alliance for the Peoples of Our America (ALBA) as a counter-hegemonic Latin American and Caribbean (LAC) regionalism. As (initially) the regionalisation of Venezuela's Bolivarian Revolution, ALBA is centred around the idea…

  19. Counter-Hegemonic Regionalism and Higher Education for All: Venezuela and the ALBA

    Science.gov (United States)

    Muhr, Thomas

    2010-01-01

    This paper employs new regionalism theory and regulatory regionalism theory in its analysis and theorisation of the Bolivarian Alliance for the Peoples of Our America (ALBA) as a counter-hegemonic Latin American and Caribbean (LAC) regionalism. As (initially) the regionalisation of Venezuela's Bolivarian Revolution, ALBA is centred around the idea…

  20. Morphogenetic responses ofPopulus alba L. under salt stress

    Institute of Scientific and Technical Information of China (English)

    Mejda Abassi; Khaled Mguis; Zoubeir Béjaoui; Ali Albouchi

    2014-01-01

    The morphogenetic responses to salt stress of TunisianPopu-lus alba clones were studied in order to promote their plantation in dam-aged saline areas. One year-old plants of threeP. alba clones (MA-104, MA-195 and OG) were subjected to progressive salt stress by irrigation during two consecutive years. The plants were grown in a nursery, inside plastic receptacles containing sandy soil and were irrigated with tap water (control) or 3-6 g/l NaCl solution. During this study, leaf epinasty, elongation rate, vigor, internode length, plant architecture, and number of buds were evaluated. Test clone response was highly dependent on the applied treatment and degree of accommodation.The most pronounced alterations were induced under 6g/l of NaCl treatment including leaf epinasty, leaf elongation rate delay, vigor decrease, internode length shortening, and morphogenetic modifications. These responses were less noticeable in the MA-104 clone with respect to the two other clones. The salt effect induced a delay in the leaf elongation rate on the MA-195 and OG clones leading to an early leaf maturity. The vigour and internode length of the MA-104 clone was less affected than the other clones. The OG clone was the most salt-sensitive thus, it developed shorter branches and more buds number than MA-195 and MA-104. The effect of long-term salt stress was to induce early flowering of theP. alba clones which suggests that mechanism of salt accommodation could be devel-oped.

  1. AKTIVITAS ANTIOKSIDAN EKSTRAK KULIT BATANG TUMBUHAN MANGROVE Sonneratia alba

    OpenAIRE

    Herawati, Netti; Jalaluddin, Noor; Daha, La; Firdaus

    2009-01-01

    Ekstrak kulit batang kayu buli berpotensi sebagai anti oksidan. Ekstrak kulit batang tumbuhan mangrove Sonneratia alba dianalisis untuk menentukan aktivitas antioksidannya dengan metode DPPH. Empat macam ekstrak menunjukkan aktivitas antioksidan yang kuat (IC50< 200 ??g/ml). Ekstrak metanol memperlihatkan aktivitas tertinggi (IC50 9.8 ??g/ml) disusul ekstrak etil asetat (IC50 10.23 ??g/ml, kloroform (IC50 27.34 ??g/ml), dan heksan ( IC50147 ??g/ml). Ekstrak metanol dan etil asetat memiliki...

  2. Antinociceptive effect of the extract of Morus nigra leaves in mice.

    Science.gov (United States)

    de Mesquita Padilha, Marina; Vilela, Fabiana Cardoso; da Silva, Marcelo José Dias; dos Santos, Marcelo Henrique; Alves-da-Silva, Geraldo; Giusti-Paiva, Alexandre

    2009-12-01

    Morus nigra has been used to relieve pain in Brazilian folk medicine. This study was conducted to establish the antinociceptive properties of dichloromethane extract from leaves of M. nigra. The formalin, hot plate, and tail immersion tests as well as acetic acid-induced writhing were used to investigate the antinociceptive activity in mice. The extract at test doses of 100 and 300 mg/kg, p.o. clearly demonstrated antinociceptive activity in all tests. The extract administered at 300 mg/kg, p.o. had a stronger antinociceptive effect than indomethacin (5 mg/kg, p.o.) and morphine (10 mg/kg, p.o.), which supports previous claims for its traditional use.

  3. Precooling and ozone treatments affects postharvest quality of black mulberry (Morus nigra) fruits.

    Science.gov (United States)

    Han, Qiang; Gao, Haiyan; Chen, Hangjun; Fang, Xiangjun; Wu, Weijie

    2017-04-15

    Mulberry (Morus spp.) fruits are delicious and nutritious, but they are highly perishable and have a very short shelf-life for sale in the market. This study investigated the effect and mechanisms of 2ppm ozone and precooling treatments on the postharvest quality of mulberry fruit during refrigerated storage. The results revealed that mulberry fruit subjected to ozone and precooling treatment had higher levels of titratable acidity and total soluble solids content, better retention in firmness and color, and lower decay rate, respiratory intensity, and polyphenol oxidase activity compared to the control. From the analysis of cell ultrastructure and cell wall components of fruit, ozone and precooling treatments also induced shrinkage of the stomata in the epidermis, inhibited bacteria invasion, reduced water transpiration, and delayed the decomposition of the cell walls and the degradation of epidermal tissues. Copyright © 2016. Published by Elsevier Ltd.

  4. Chaenomeles japonica, Cornus mas, Morus nigra fruits characteristics and their processing potential.

    Science.gov (United States)

    Tarko, Tomasz; Duda-Chodak, Aleksandra; Satora, Paweł; Sroka, Paweł; Pogoń, Piotr; Machalica, Justyna

    2014-12-01

    The aim of this study was to determine the suitability of Japanese quince (Chaenomeles japonica), cornelian cherry (Cornus mas) and black mulberry (Morus nigra) fruits as raw materials for processing. All analyzed fruits were characterized by high antioxidant activity and total phenolic content. Fruits of Japanese quince and cornelian cherry had also high acidity. Products such as fruit wines and liquors were prepared from the tested fruits. In respect of soluble solid content and reducing sugars content the obtained wine should be classified as dry wines. All analyzed liquors were characterized by similar alcohol content and the soluble solid content. Moreover, liquors made from Japanese quince and cornelian cherry were characterized by high total acidity and antioxidant capacity. The results of sensory analysis showed high commercial potential of the examined fruits.

  5. Kara Dut (Morus nigra L.) Bitkisinin In Vitro Çoğaltımı

    OpenAIRE

    KALKIŞIM, Özgün; Turan, Ali; AZERİ, Fatma Nur; ÖZDEŞ, Duygu

    2014-01-01

    Karadutun (Morus nigra L.) doku kültürü ile çoğaltılabilme imkanlarını belirlemek amacıyla yapılan bu çalışmada, erişkin ağaçların obur dallarından alınan nodal eksplantlardan çoklu sürgün teşvikini sağlamaya yönelik etkili bir protokol standardize edilmek üzere, bitki büyüme düzenleyicilerin farklı konsantrasyon ve kombinasyonu ile zenginleştirilmiş MS ortamları denenmiştir. Beş ayrı konsantrasyonda BAP (6-enzylaminopurine; 1,0, 2,0, 3,0, 4,0, 5,0 mg/L) ile desteklenmiş ve ayrıca BAP, NAA (...

  6. Hepatoprotective activity of aqueous methanolic extract of Morus nigra against paracetamol-induced hepatotoxicity in mice

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    Tauqeer Hussain Mallhi

    2014-03-01

    Full Text Available Morus nigra (Family Moraceae is traditionally used injaundice, diabetes, hypertension, cough, fever and cancer. The current study was conducted to determine hepatoprotective activity of aqueous methanolic extract of leaves of M. nigra. Two doses of 250 mg/kg p.o and 500 mg/kg p.o showed that extract of M. nigra produced significant (p<0.001 reduction in liver enzymes (ALT, AST, ALP and total bilirubin induced by paracetamol and the results are comparable to silymarin (p<0.001. Results were supported by histopathologi-cal investigations, phytochemical screening and detection of active consti-tuents by HPLC. The current study showed that aqueous methanolic extract of M. nigra possess hepatoprotective activity that might be due to quercetin, luteolin and isorhamnetin. It was concluded from this study that M. nigra has hepatoprotective activity against paracetamol induced liver injury in mice.

  7. Physical and chemical characterization of fruits of mulberry cultivars from genus Morus

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    Fumiko Okamoto

    2012-02-01

    Full Text Available This study aimed to evaluate the physical and chemical characteristics of mulberry cultivars fruits (Morus spp.. The experiment was conducted at Marilia, State of Sao Paulo, Brazil, it was evaluated fruits of ten mulberry cultivars, IZ 1/1, IZ 1/2, IZ 1/3, IZ 1/12, IZ 2/1, IZ 3/1, IZ 4/1, IZ 15/1, Korin and Miura. The physical and chemical characteristics of mulberry fruits varied according to cultivar. Cultivar Korin stood out because showed higher soluble solids content and higher acidity, important requirements for better tasting fruit. When considering the volume for processing, the cultivars IZ 1/3, IZ 1/12 and IZ 15/1 had a higher mass.

  8. Locational differences in metal concentrations in feathers of Australasian gannet (Morus serrator) in New Zealand.

    Science.gov (United States)

    Burger, J; Veitch, C R; Gochfeld, M

    1994-08-01

    Levels of lead, cadmium, mercury, selenium, manganese and chromium were examined in breast feathers of Australasian Gannets (Morus serrator) from New Zealand waters, where they breed on coastal rocky stacks, islands, and occasionally coastal headlands. There were significant differences in the concentrations of all metals except mercury, with gannets from Ninety Mile Beach having significantly lower levels of all metals than those from the other beaches. Ninety Mile Beach is on the northern tip of the North Island, away from urbanization and industrialization, whereas the other beaches are closer to Auckland, the largest city in New Zealand. The levels of most metals were similar or lower than those reported for feathers from elsewhere around the world. Mercury, however, was at the high end, being exceeded only by raptors and gulls from industrialized countries. Several metal levels were highly correlated, indicating that gannets were usually exposed to several metals at once.

  9. Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Ibrahim Turan

    2017-02-01

    Full Text Available Background: Morus nigra L. belongs to the family Moraceae and is frequently used in traditional medicine. Numerous studies have investigated the antiproliferative effects of various extracts of different Morus species, but studies involving the in vitro cytotoxic effect of M. nigra extract are very limited. The purpose of this study was to evaluate the phenolic composition and antioxidant activity of dimethyl sulfoxide extract of M. nigra (DEM and to investigate, for the first time, the probable cytotoxic effect in human prostate adenocarcinoma (PC-3 cells together with the mechanism involved. Methods: Total polyphenolic contents (TPC, ferric reducing antioxidant power (FRAP and phenolic compounds of DEM were evaluated using spectrophotometric procedures and HPLC. The cytotoxic effect of DEM on PC-3 cells was revealed using the MTT assay. Mechanisms involved in the cytotoxic effect of DEM on PC-3 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, while caspase activity was investigated using luminometric analysis. Results: TPC and FRAP values were 20.7 ± 0.3 mg gallic acid equivalents and 48.8 ± 1.6 mg trolox equivalents per g sample, respectively. Ascorbic acid and chlorogenic acid were the major phenolic compounds detected at HPLC analysis. DEM arrested the cell cycle of PC-3 cells at the G1 phase, induced apoptosis via increased caspase activity and reduced mitochondrial membrane potential. Conclusions: Our results indicate that M. nigra may be a novel candidate for the development of new natural product based therapeutic agents against prostate cancer.

  10. Induction of Biologically Active Flavonoids in Cell Cultures of Morus nigra and Testing their Hypoglycemic Efficacy.

    Science.gov (United States)

    Abd El-Mawla, Ahmed M A; Mohamed, Khaled M; Mostafa, Ashraf M

    2011-01-01

    The antidiabetic activity of both leaves and MJ-treated cell cultures of Morus nigra was evaluated after their oral administration to streptozotocin-induced diabetic rats. The antidiabetic activity of extracts from leaves given to streptozotocin (STZ)-diabetic rats for 10 days increased with increasing doses of leaves extract up to 500 mg/kg/day. The administration of 500 mg/kg/day of leaves extract reduced the concentration of glucose from 370 ± 7.31 mg/dl (control) to 154 ± 6.27 mg/dl, and a significant increase in the insulin level from 11.3 ± 0.31 μU/ml (control) to 14.6 ± 0.43 μU/ml was recorded. Cell suspension cultures were established from the young leaves of Morus nigra cultivated on modified MS medium supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA), 0.2 mg/l 6-(furfurylamino)purine (kinetin). The changes in cell weight and flavonoid content were monitored between day zero and 12. The linear increase in fresh weight was found to be parallel to flavonoids production. Cell cultures treated with 100 μM methyl jasmonate for 24 hours showed a noticeable increase in level of flavonoids and significant and more effective hypoglycemic activity than that for extract from leaves. The major flavonoids were isolated by TLC and HPLC and identified as rutin, quercetin, Morusin and cyclomorusin by co-chromatography and mass spectrometry in comparison to samples of authentic reference compounds.

  11. Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells.

    Science.gov (United States)

    Turan, Ibrahim; Demir, Selim; Kilinc, Kagan; Burnaz, Nesibe Arslan; Yaman, Serap Ozer; Akbulut, Kubra; Mentese, Ahmet; Aliyazicioglu, Yuksel; Deger, Orhan

    2017-02-01

    Background: Morus nigra L. belongs to the family Moraceae and is frequently used in traditional medicine. Numerous studies have investigated the antiproliferative effects of various extracts of different Morus species, but studies involving the in vitro cytotoxic effect of M. nigra extract are very limited. The purpose of this study was to evaluate the phenolic composition and antioxidant activity of dimethyl sulfoxide extract of M. nigra (DEM) and to investigate, for the first time, the probable cytotoxic effect in human prostate adenocarcinoma (PC-3) cells together with the mechanism involved. Methods: Total polyphenolic contents (TPC), ferric reducing antioxidant power (FRAP) and phenolic compounds of DEM were evaluated using spectrophotometric procedures and HPLC. The cytotoxic effect of DEM on PC-3 cells was revealed using the MTT assay. Mechanisms involved in the cytotoxic effect of DEM on PC-3 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, while caspase activity was investigated using luminometric analysis. Results: TPC and FRAP values were 20.7 ± 0.3 mg gallic acid equivalents and 48.8 ± 1.6 mg trolox equivalents per g sample, respectively. Ascorbic acid and chlorogenic acid were the major phenolic compounds detected at HPLC analysis. DEM arrested the cell cycle of PC-3 cells at the G1 phase, induced apoptosis via increased caspase activity and reduced mitochondrial membrane potential. Conclusions: Our results indicate that M. nigra may be a novel candidate for the development of new natural product based therapeutic agents against prostate cancer.

  12. Pityriasis alba revisited: perspectives on an enigmatic disorder of childhood.

    Science.gov (United States)

    Jadotte, Yuri T; Janniger, Camila K

    2011-02-01

    Pityriasis alba (PA) is a localized hypopigmented disorder of childhood with many existing clinical variants. It is more often detected in individuals with a darker complexion but may occur in individuals of all skin types. Atopy, xerosis, and mineral deficiencies are potential risk factors. Sun exposure exacerbates the contrast between normal and lesional skin, making lesions more visible and patients more likely to seek medical attention. Poor cutaneous hydration appears to be a common theme for most risk factors and may help elucidate the pathogenesis of this disorder. The end result of this mechanism is inappropriate melanosis manifesting as hypopigmentation. It must be differentiated from other disorders of hypopigmentation, such as pityriasis versicolor alba, vitiligo, nevus depigmentosus, and nevus anemicus. Alleviation of the various risk factors via patient education on proper skin care and hygiene, use of lubricants and emollients, topical corticosteroid therapy in the presence of inflammation, and the novel administration of topical anti-inflammatory drugs such as calcineurin inhibitors can play a crucial role in promoting remission or resolution.

  13. Mode of antibacterial activity of Eclalbasaponin isolated from Eclipta alba.

    Science.gov (United States)

    Ray, A; Bharali, P; Konwar, B K

    2013-12-01

    The present study was undertaken to evaluate the mode of antibacterial activity of Eclalbasaponin isolated from Eclipta alba, against selected Gram-positive and Gram-negative bacteria. The probable chemical structure was determined by using various spectroscopic techniques such as Fourier transform infrared spectroscopy (FTIR) and mass spectroscopy. The antibacterial activity was evaluated by well diffusion technique, pH sensitivity, chemotaxis, and crystal violet assays. Eclalbasaponin showed clear zone of inhibition against both Bacillus subtilis and Pseudomonas aeruginosa and exhibited growth inhibition at the pH range of 5.5-9.0. The isolated saponin exhibited its positive chemoattractant property for both bacterial strains. Results of crystal violet assay and the presence of UV-sensitive materials in the cell-free supernatant confirmed the cellular damages caused by the treatment of Eclalbasaponin. The release of intracellular proteins due to the membrane damage was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Changes in the cell surface structure and membrane disruption were further revealed by FTIR and scanning electron microscopy analysis. The present study suggests that the isolated saponin from E. alba causes the disruption of the bacterial cell membrane which leads to the loss of bacterial cell viability.

  14. Ecologia da polinização da amoreira-preta (Rubus sp (Rosaceae em Timbó-SC, Brasil Pollination ecology of blackberry (Rubus sp. (Rosaceae in Timbo (SC, Brazil

    Directory of Open Access Journals (Sweden)

    Leônidas João de Mello Junior

    2011-09-01

    Full Text Available O presente trabalho verificou experimentalmente a ecologia da polinização da amoreira-preta (Rubus sp.. Objetivou-se confirmar a síndrome de polinização de Rubus sp., por meio de análise de seu sistema reprodutivo, quantificação da produção diária de néctar, levantamento da entomofauna que visita as flores da amoreira na área de estudo e análise do seu comportamento polinizador. Os experimentos foram realizados em uma área de cultivo de amoreira-preta, no mês de dezembro de 2005, município de Timbó (SC. Constatou-se que a maior taxa de frutificação (48,3 % ± 3,2 ocorreu via polinização livre, onde os insetos não foram excluídos. Foi verificada a ocorrência de autopolinização, porém com taxa inferior de frutificação (12,2% ± 4,9. A anemofilia não foi constatada na espécie. A avaliação da produção de néctar em Rubus sp. resultou em valores compatíveis com a síndrome de melitofilia. Os visitantes florais coletados e observados sobre as flores de Rubus sp. foram predominantemente abelhas da ordem Hymenoptera (97%, que iniciam, em menor número, a atividade de forrageamento às 8h, com pico de atividade às 12h e declinando até às 16h. As coletas resultaram numa amostragem de 1.360 abelhas, divididas em quatro famílias e 13 espécies. A família com maior riqueza de espécies (N = 7 foi Halictidae e a mais abundante foi Apidae, com 1.288 indivíduos. Em Apidae, houve o predomínio da espécie exótica Apis mellifera, cuja representação na amostra foi de 1.246 indivíduos. Os dados da abundância e as observações naturalísticas do seu comportamento da flor apontaram A. mellifera como o principal polinizador de Rubus sp., mas confirmam que as demais espécies de abelhas também participam na polinização de Rubus sp.The pollination ecology of the blackberry (Rubus sp. was studied by means of determining its reproductive system, the analysis of flower's nectar production. Sampling the insects that visit

  15. The mosaic of ancestral karyotype blocks in the Sinapis alba L. genome.

    Science.gov (United States)

    Nelson, Matthew N; Parkin, Isobel A P; Lydiate, Derek J

    2011-01-01

    The organisation of the Sinapis alba genome, comprising 12 linkage groups (n = 12), was compared with the Brassicaceae ancestral karyotype (AK) genomic blocks previously described in other crucifer species. Most of the S. alba genome falls into conserved triplicated genomic blocks that closely match the AK-defined genomic blocks found in other crucifer species including the A, B, and C genomes of closely related Brassica species. In one instance, an S. alba linkage group (S05) was completely collinear with one AK chromosome (AK1), the first time this has been observed in a member of the Brassiceae tribe. However, as observed for other members of the Brassiceae tribe, ancestral genomic blocks were fragmented in the S. alba genome, supporting previously reported comparative chromosome painting describing rearrangements of the AK karyotype prior to the divergence of the Brassiceae from other crucifers. The presented data also refute previous phylogenetic reports that suggest S. alba was more closely related to Brassica nigra (B genome) than to B. rapa (A genome) and B. oleracea (C genome). A comparison of the S. alba and Arabidopsis thaliana genomes revealed many regions of conserved gene order, which will facilitate access to the rich genomic resources available in the model species A. thaliana for genetic research in the less well-resourced crop species S. alba.

  16. Hypocholesterolemic and Antiatherosclerotic Potential of Basella alba Leaf Extract in Hypercholesterolemia-Induced Rabbits

    Directory of Open Access Journals (Sweden)

    Gunasekaran Baskaran

    2015-01-01

    Full Text Available Hypercholesterolemia is the major risk factor that leads to atherosclerosis. Nowadays, alternative treatment using medicinal plants gained much attention since the usage of statins leads to adverse health effects, especially liver and muscle toxicity. This study was designed to investigate the hypocholesterolemic and antiatherosclerotic effects of Basella alba (B. alba using hypercholesterolemia-induced rabbits. Twenty New Zealand white rabbits were divided into 5 groups and fed with varying diets: normal diet, 2% high cholesterol diet (HCD, 2% HCD + 10 mg/kg simvastatin, 2% HCD + 100 mg/kg B. alba extract, and 2% HCD + 200 mg/kg B. alba extract, respectively. The treatment with B. alba extract significantly lowered the levels of total cholesterol, LDL, and triglycerides and increased HDL and antioxidant enzymes (SOD and GPx levels. The elevated levels of liver enzymes (AST and ALT and creatine kinase were noted in hypercholesterolemic and statin treated groups indicating liver and muscle injuries. Treatment with B. alba extract also significantly suppressed the aortic plaque formation and reduced the intima: media ratio as observed in simvastatin-treated group. This is the first in vivo study on B. alba that suggests its potential as an alternative therapeutic agent for hypercholesterolemia and atherosclerosis.

  17. Potential antidiabetic and antioxidant activities of Morus indica and Asystasia gangetica in alloxan-induced diabetes mellitus.

    Science.gov (United States)

    Kumar, R Pradeep; Sujatha, D; Saleem, Ts Mohamed; Chetty, C Madhusudhana; Ranganayakulu, D

    2010-01-01

    Herbal drugs are frequently considered to be less toxic and also free from side effects, than synthetic ones. Hence, the present study was designed to investigate one such combination of herbal drugs, Asystasia gangetica and Morus indica for their antidiabetic and antioxidant potential against alloxan-induced diabetes in albino rats. The effect of both individual and a combination of Asystasia gangetica and Morus indica on blood glucose and liver glycogen were studied in the diabetic rats. The study also assessed for the effect of selected plant extracts for their effect on Superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and Lipid peroxidation (LPO) in the homogenates of the pancreas. The results of the present study attests significant antidiabetic and antioxidant potential for the selected plants individually and also in combination as a prominent decrease in blood glucose and liver glycogen was observed in the rats treated with the extracts of the selected plants. Similarly, the levels of the protective antioxidant enzymes like SOD, CAT and GSH were increased along with decrease in the LPO levels. The present study provides a scientific evidence for antidiabetic and antioxidant potential of Asystasia gangetica and Morus indica. Further studies to isolate bioactive compounds will pave the way to identify potential lead compounds for developing safe and efficacious antidiabetic agents.

  18. Adventitious bud regeneration from the stigma of Sinapis alba L.

    Directory of Open Access Journals (Sweden)

    Elżbieta Zenkteler

    2012-12-01

    Full Text Available Stigmas isolated from flower buds of 'Nakielska' variety of Sinapis alba were used to develop a micropropagation method suitable for breeding of new cultivars. The origin of adventitious bud regeneration was studied on MS medium, under stimulation by bezylaminopurine (BAP in combination with 2,4-D - dichlorophenoxyacetic acid (2,4-D. Histological analysis showed the structure of Sinapis stigma (composed from four types of tissue: papillae, transmitting tissue, parenchyma and vascular bundles and revealed that numerous meristematic centers developed from parenchyma cells in close vicinity of vascular bundles. Buds very quickly appeared on the surface of initial explants and later formed multiplantlets that were easily rooted in the soil.

  19. Alba Longa, histoire d’une légende

    OpenAIRE

    Grandazzi, Alexandre

    2013-01-01

    Alba Longa et sa légende demeurent pour la science une énigme : alors qu’elle explore les origines de Rome avec une intensité sans précédent, elle ne sait que penser de cette ville introuvable, de ce lac débordant, de ces forêts ombreuses, de ces rois fantômes. Quel rôle jouèrent les monts Albains dans la culture latiale ? A-t-on eu raison, depuis plus de cinq siècles, de chercher inlassablement Albe, cette cité d’où seraient venus les jumeaux fondateurs de Rome ? Quelles furent les relations...

  20. Morphometry of auricular feathers of barn owls (Tyto alba).

    Science.gov (United States)

    Koch, U R; Wagner, H

    2002-02-01

    In all owl species, the facial plumage forms a parabolic dish, the facial ruff, which is most conspicuous in the the barn owl (Tyto alba). The center of the ruff is formed by auricular feathers. Such feathers are also found on the preaural flaps which cover the ear openings, and in the region of the beak. In this study, we compare the different types of auricular feathers of the barn owl with contour feathers from the neck. Auricular feathers are characterised by an open vane structure and fewer barbs as compared to contour feathers. Auricular feathers also have fewer distal and proximal barbules than contour feathers. The open vane of the auricular feather results from an acute angle between the barb and the basis of the barbules, and from the extension of the pennula parallel to the barbs. These reductions are differently expressed in the three different types of auricular feathers investigated here and correspond with their function (protecting the ruff from dust).

  1. Radioactively labelled phytic acid from maturing seeds of Sinapis alba

    Energy Technology Data Exchange (ETDEWEB)

    Blaicher, F.M.; Mukherjee, K.D.

    Maturing seeds of Sinapis alba were incubated with D-(U-/sup 14/C)glucose, sodium (1-/sup 14/C) acetate or myo-(U/sup 14/C) inositol in order to prepare radioactively labelled phytic acid with high specific activity. Although each substrate was utilized for the biosynthesis of phytic acid, maximum incorporation of radioactivity into phytic acid was found with myo-inositol. Radiochemical purity of the (U-/sup 14/C)phytic acid preparations was confirmed by chromatographic techniques. Such preparations should be useful for the study of interaction of phytic acid with metal ions and proteins and may serve as substrate in the assay should be useful for the study of interaction of phytic acid with metal ions and proteins and may serve as substrate in the assay of phytase.

  2. Triterpenoid saponins from Lippia alba (Mill.) N. E. Brown

    Energy Technology Data Exchange (ETDEWEB)

    Farias, Mareni R.; Pertile, Roberto; Correa, Melissa M.; Schenkel, Eloir P., E-mail: marenif@yahoo.com.b [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil). Dept. de Ciencias Farmaceuticas. Programa de Pos-graduacao em Farmacia; Almeida, Maria Tereza R. de; Palermo, Jorge A. [Universidad de Buenos Aires (Argentina). Facultad de Ciencias Exactas y Naturales. Dept. de Quimica Organica

    2010-07-01

    Two saponins were isolated from the leaves of Lippia alba. Their structures were established using one- and two-dimensional NMR spectroscopy and mass spectrometry. These new compounds were elucidated as 3-O-beta-D-glucopyranosyl-28-O-(alpha-L-rhamnopyranosyl-(1->3)-beta-D-xylopyranosyl -(1->4)-alpha-L-rhamnopyranosyl-(1->2)-alpha-L-arabinopyranosyl)-16alpha, 23-dihydroxy-olean -12-en-28-oic acid, named as Lippiasaponin I (2) and as 3-O-beta-D-glucopyranosyl-28-O-(alpha-L-rhamnopyranosyl-(1->3)-beta-D-xylopyranosyl- (1->4)-alpha-L-rhamnopyranosyl-(1->3)-alpha-Larabinopyranosyl)-16alpha,23-dihydroxy-olean -12-en-28-oic acid, named Lippiasaponin II (3). (author)

  3. Chemical Diversity in Lippia alba (Mill.) N. E. Brown Germplasm

    Science.gov (United States)

    Camêlo, Lídia Cristina Alves; Pinheiro, José Baldin; Andrade, Thiago Matos; Alves, Péricles Barreto

    2015-01-01

    The aim of this study was to perform chemical characterization of Lippia alba accessions from the Active Germplasm Bank of the Federal University of Sergipe. A randomized block experimental design with two replications was applied. The analysis of the chemical composition of the essential oils was conducted using a gas chromatograph coupled to a mass spectrometer. The chemical composition of the essential oils allowed the accessions to be allocated to the following six groups: group 1: linalool, 1,8-cineole, and caryophyllene oxide; group 2: linalool, geranial, neral, 1,8-cineol, and caryophyllene oxide; group 3: limonene, carvone, and sabinene; group 4: carvone, limonene, g-muurolene, and myrcene; group 5: neral, geranial, and caryophyllene oxide; and group 6: geranial, neral, o-cymene, limonene, and caryophyllene oxide. PMID:26075292

  4. Atividade antimicrobiana de Lippia alba (Mill. N. E. Brown (Verbenaceae Antimicrobial activity of Lippia alba (Mill. N. E. Brown (Verbenaceae

    Directory of Open Access Journals (Sweden)

    Jaciana S. Aguiar

    2008-09-01

    Full Text Available Lippia alba (Mill. N. E. Brown (Verbenaceae, amplamente distribuída em todo o território brasileiro, é conhecida popularmente como erva cidreira e utilizada na medicina popular como analgésica, febrífuga, antiinflamatória, antigripal e nas afecções hepáticas. Extratos brutos foram preparados a partir de plantas cultivadas, de modo padronizado, em horta medicinal do Laboratório de Fitoterapia da Empresa Pernambucana de Pesquisa Agropecuária (IPA para a verificação da atividade antimicrobiana, in vitro, pelo método de difusão em disco de papel. A concentração inibitória mínima (CIM foi determinada para os extratos que exibiram melhores atividades. Os resultados obtidos mostraram que os extratos clorofórmico, acetônico e etanólico da raiz foram ativos frente a Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Mycobacterium smegmatis, Candida albicans e Monilia sitophila e os extratos hexânicos, etanólicos e metanólicos das folhas inibiram S. aureus, M. luteus, B. subtilis, M. smegmatis e M. sitophila. A menor concentração inibitória (CIM = 31,2 µg/mL, foi obtida para o extrato clorofórmico da raiz frente a B. subtilis e M. luteus.Lippia alba (Mill. N. E. Brown (Verbenaceae, commonly known as "erva cidreira", is widely distributed throughout Brazil and is used in folk medicine as an analgesic, anti-inflammatory, cold remedy, as well as to reduce fevers and treat hepatic afflictions. Crude extracts of L. alba were prepared from plants cultivated in the medicinal garden of the Laboratório de Fitoterapia of the Empresa Pernambucana de Pesquisa Agropecuária (IPA, State of Pernambuco, Brazil, using standard techniques to test their in vitro antibacterial activity using the paper disk-diffusion method. The minimum inhibitory concentration (MIC was determined for those extracts demonstrating the highest activity. The results demonstrated that the chloroform, acetone and ethanol extracts of root material were

  5. Effect of Eclipta alba on acute seizure models: a GABAA-mediated effect

    Directory of Open Access Journals (Sweden)

    M F Shaikh

    2013-01-01

    Full Text Available In the present study, anticonvulsant activity of methanol extract of Eclipta alba (10-200 mg/kg was studied using pentylenetetrazole- and picrotoxin-induced seizure models. Mechanism of effect of methanol extract of Eclipta alba was further elucidated by studying its GABA A receptor modulatory activity and its effect on levels of GABA in mice brain. Methanol extract of Eclipta alba exhibited potent anticonvulsant activity but has saturation of its pharmacological activity at 50 mg/kg. At the concentration of 10 mg/ml, contractions induced in guinea pig ileum was blocked by picrotoxin, but it didn′t not show any increase in GABA levels in mice brain after treatment. Hence, it can be concluded that methanol extract of Eclipta alba possesses potent anticonvulsant activity because of its positive modulatory effect on GABA A receptors.

  6. Accentuating the prodigious significance of Eclipta alba - an inestimable medicinal plant.

    Science.gov (United States)

    Sidra, Sidra; Hussain, Shahzad; Malik, Farnaz

    2013-11-01

    Eclipta alba is a small branched perennial herb, which has been used as a traditional medicine in different countries mainly in tropical and subtropical regions of the world. The plant E. alba plays a significant role in the ayurvedic, traditional and unani systems of medicine. It is popularly known as "Bhringaraj". The herb has been known for its medicinal value and has been used as an analgesic, antimytotoxic, antihepatotoxic, antibacterial, antioxidant, antihaemorrhagic, antihyperglycemic and immunomodulatory and also recognized as a reincarnated plant. Broad range of chemical constituents have been detached from E. alba including coumestans, alkaloids, thiopenes, flavonoids, polyacetylenes, triterpenes and their glycosides. Pharmacological activities have been seen in the metabolites and extracts of this plant. Therefore this herb produces robust curative lead compounds, which would be propitious for humanity. The purpose of this review recapitulates all data related to E. alba considering its prodigious medicinal importance.

  7. Tissue immunostaining for factor XIIIa in dermal dendrocytes of pityriasis alba skin lesions

    National Research Council Canada - National Science Library

    Carneiro, Francisca Regina Oliveira; Amaral, Gabriela Borborema do; Mendes, Maiana Darwich; Quaresma, Juarez Antônio Simões

    2014-01-01

    .... However, its etiology remains uncertain. OBJECTIVE: The objective of the present study was to evaluate the immunoexpression of factor XIIIa in dermal dendrocytes of skin lesions of pityriasis alba. METHOD...

  8. DEVELOPMENT POLICIES IN ALBA IULIA AREA OF INFLUENCE. AN INTEGRATED APPROACH

    Directory of Open Access Journals (Sweden)

    S. A. NICULA

    2015-06-01

    Full Text Available Development Policies in Alba Iulia Area of Influence. An Integrated Approach. The paper represents an integrated and holarchical perspective on the spatial development policies and its component measures and projects related to the City of Alba Iulia, its area of influence and the all-encompassing County of Alba, Romania. The goal was to see how the development and management policies from all levels merge into a single strategic framework that might create a favourable basis for the sustainable growth of Alba Iulia and its area of influence. As this area surrounding the city is subjected to different hierarchical plans and programmes, some that are not properly correlated, it is extremely clear that this area and Areas of Influence in general need legislative stipulations made specifically for them and also a well-thought holarchical planning approach.

  9. Chemical characterization of Lippia alba essential oil: an alternative to control green molds

    OpenAIRE

    Jasmina Glamočlija; Marina Soković; Vele Tešević; Giani Andrea Linde; Nelson Barros Colauto

    2011-01-01

    The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure ...

  10. Atividade antinociceptiva do extrato etanólico das folhas de Morus nigra L. (Moraceae

    Directory of Open Access Journals (Sweden)

    Grasielly Rocha Souza

    2015-09-01

    Full Text Available O extrato etanólico bruto das folhas de M. nigra (Mn-EtOH foi avaliado quanto à atividade antinociceptiva em modelos experimentais em camundongos. Foram usados os métodos de contorções abdominais induzidas pelo ácido acético, teste da formalina e placa quente. No teste de contorções abdominais induzidas pelo ácido acético, Mn-EtOH (100, 200 e 400 mg/kg, i.p. reduziu o número de contorções em 38,82, 97,65 e 100%, respectivamente. O extrato também produziu uma significativa inibição de ambas as fases do teste da formalina, sendo que o efeito foi mais significativo na segunda fase (dor inflamatória. O extrato diminuiu em 42,19, 56,96 e 40,50%, respectivamente, o tempo de lambida da pata na primeira fase do teste de formalina, assim como 84,04, 77,20 e 61,07%, respectivamente, na segunda fase. Além disso, não apresentou efeito no teste da placa quente. Os dados obtidos sugerem que o extrato tem efeito antinociceptivo, provavelmente mediado através de mecanismos periféricos. Novos estudos estão sendo realizados para caracterizar o mecanismo responsável por esse efeito.Palavras-chave: Morus nigra. Moraceae. Atividade antinociceptiva. Dor. ABSTRACTAntinociceptive activity of ethanolic extract of the leaves of Morus nigra L. (MoraceaeThe crude ethanolic extract of the leaves of M. nigra (Mn-EtOH was evaluated for antinociceptive activity in mice using models of nociception. The evaluation of antinociceptive activity was carried out by the acetic acid-induced writhing, formalin and hot plate tests. In the acetic acid-induced writhing test, the Mn-EtOH (100, 200 and 400 mg/kg, i.p. reduced the number of writhing by 38.82, 97.65 and 100%, respectively. The extract also produced a significant inhibition of both phases of the formalin test, and the effect was more significant in the second phase (inflammatory pain. The extract decreased by 42.19, 56.96 and 40.50%, respectively, the paw licking time in the first phase of

  11. Tissue immunostaining for factor XIIIa in dermal dendrocytes of pityriasis alba skin lesions*

    Science.gov (United States)

    Carneiro, Francisca Regina Oliveira; do Amaral, Gabriela Borborema; Mendes, Maiana Darwich; Quaresma, Juarez Antônio Simões

    2014-01-01

    BACKGROUND Pityriasis alba affects 1% of the world population and about 9.9% of the children in Brazil. However, its etiology remains uncertain. OBJECTIVE The objective of the present study was to evaluate the immunoexpression of factor XIIIa in dermal dendrocytes of skin lesions of pityriasis alba. METHOD Twenty patients with pityriasis alba and 20 patients with atopic dermatitis underwent biopsy. The dermal dendrocytes marked by factor XIIIa were counted by means of immunohistochemical analysis. RESULTS The mean amount of dermal dendrocytes found in the patients with pityriasis alba was 2, whereas in the patients with atopic dermatitis it was 4, with a statistically significant difference between them. A cutoff point of 3 cells/square inch was established to differentiate pityriasis alba from atopic dermatitis, with 80% sensibility and 90% specificity. CONCLUSION We believe that pityriasis alba and atopic dermatitis should be considered different clinical forms within the spectrum of atopic disease, in which sun radiation plays an important role by modulating the progression of the disease. PMID:24770500

  12. Tissue immunostaining for factor XIIIa in dermal dendrocytes of pityriasis alba skin lesions.

    Science.gov (United States)

    Carneiro, Francisca Regina Oliveira; Amaral, Gabriela Borborema do; Mendes, Maiana Darwich; Quaresma, Juarez Antônio Simões

    2014-01-01

    Pityriasis alba affects 1% of the world population and about 9.9% of the children in Brazil. However, its etiology remains uncertain. The objective of the present study was to evaluate the immunoexpression of factor XIIIa in dermal dendrocytes of skin lesions of pityriasis alba. Twenty patients with pityriasis alba and 20 patients with atopic dermatitis underwent biopsy. The dermal dendrocytes marked by factor XIIIa were counted by means of immunohistochemical analysis. The mean amount of dermal dendrocytes found in the patients with pityriasis alba was 2, whereas in the patients with atopic dermatitis it was 4, with a statistically significant difference between them. A cutoff point of 3 cells/square inch was established to differentiate pityriasis alba from atopic dermatitis, with 80% sensibility and 90% specificity. We believe that pityriasis alba and atopic dermatitis should be considered different clinical forms within the spectrum of atopic disease, in which sun radiation plays an important role by modulating the progression of the disease.

  13. Eclipta Alba as Corrosion Pickling Inhibitor on Mild Steel in Hydrochloric Acid

    Institute of Scientific and Technical Information of China (English)

    M. Shyamala; A. Arulanantham

    2009-01-01

    Due to ease of application, cost effectiveness and environmentally safe, in this study, the corrosion inhibition effect of aqueous extract of Eclipta alba in 1 N hydrochloric acid has been investigated by weight loss, potentiodynamic polarization and impedance methods and the extracts of Eclipta alba were found to be effective corrosion pickling inhibitor. The effect of immersion time and temperature revealed that the extracts of Eclipta alba with an optimum concentration of 8.0% v/v showed maximum inhibition efficiency of 99.6% at 3 h immersion time and 30℃. Arrhenius plots for mild steel immersed in 1 N HCI solution in the absence and presence of optimum concentration (8.0% in v/v) of Eclipta alba extract showed the effect of temperature. Polarization studies indicate that this plant extract acts as a mixed type inhibitor. The adsorption of Eclipta alba follows Langmuir adsorption isotherm. The inhibition action may be due to the presence of the Wedelactone and also the alkaloid Ecliptine present in the leaves of Eclipta alba.

  14. Concentrações de ácido indolbutírico e períodos de escuro, no enraizamento "in vitro" de amoreira-preta (Rubus sp., cv. ébano

    Directory of Open Access Journals (Sweden)

    Radmann Elizete Beatriz

    2003-01-01

    Full Text Available O trabalho foi realizado com o objetivo de testar a influência do AIB em condições de escuro, no enraizamento in vitro de amoreira-preta cv. Ébano. Utilizaram-se explantes provenientes da propagação in vitro, os quais foram submetidos a duas concentrações de ácido indolbutírico (AIB (0,5 e 1,0 mM e três períodos de escuro (2; 4 e 6 dias. Os explantes foram cultivados em meio contendo sais e vitaminas de MS (Murashige & Skoog, 1962 com os sais minerais reduzidos à metade, acrescidos de mio-inositol (100 mg.L-1, sacarose (30 g.L-1, ágar (7g.L-1 e o pH ajustado para 5,9. Os frascos contendo os explantes, após o tratamento de escuro, foram incubados em sala de crescimento com fotoperíodo de 16 horas, densidade de fluxo luminoso de 31,41W.m-2 e temperatura de 25±3ºC, por 30 dias. Avaliaram-se a porcentagem de enraizamento, número e comprimento de raízes, e formação de calo na base das brotações. Após os explantes serem aclimatizados, avaliou-se a taxa de sobrevivência. Não houve diferenças significativas entre as porcentagens de enraizamento dos diferentes tratamentos. O número de raízes foi maior em meios sem ácido indolbutírico (5,5 raízes/ explante. As raízes mais longas foram observadas em meios sem ácido indolbutírico e quando submetidas 2-4 dias no escuro. Ocorreu maior intensidade de formação de calo quando adicionado ácido indolbutírico ao meio de cultura. Verificou-se alta porcentagem de sobrevivência das brotações na fase de aclimatização (86 - 97%.

  15. Avaliação da atividade fitoestrogênica do extrato hidroalcoólico e da infusão das folhas de Morus nigra L.

    OpenAIRE

    Ana Paula Nunes Bitencourt Vanoni

    2006-01-01

    Avaliou-se a influência do extrato hidroalcoólico e da infusão das folhas de Morus nigra L. em diferentes ensaios biológicos (uterotrófico em ratas adultas ovariectomizadas e em pré-púberes, e pubertal) em ratas Wistar. O objetivo dos ensaios foi avaliar a atividade estrogênica de Morus nigra observando-se o desenvolvimento uterino de ratas adultas ovariectomizadas e pré-púberes, e, a manifestação da abertura do canal vaginal, regularidade do ciclo estral e desenvolvimento uterino de ratas do...

  16. Effect of geographical distributions on the nutrient composition, phytochemical profile and antioxidant activity of Morus nigra.

    Science.gov (United States)

    Khattak, Khanzadi Fatima; Rahman, Tajur Rahman

    2015-09-01

    Recent worldwide inclination for the consumption of natural compounds has extremely augmented the significance of persistent quality of plant materials. Consequently, there is an escalating scientific concern in the impact of geographical distributions of the plants on their chemical constituents, physical characteristics and biological activities. The current study was carried out to see the effect of geographical locations on the nutrient composition, mineral contents, phytochemical profile and free radical scavenging activity of Morus nigra fruit. The samples were collected from five different locations of Khyber Pakhtunkhwa, which included districts of D. I. Khan, Karak, Peshawar, Swabi and Swat. The results revealed the considerable impact of geographical locations on the levels of proximate nutrient and selected minerals. Likewise, the concentrations of phenolic, flavonoid, anthocyanin and alkaloidal contents varied significantly (p<0.05) with respect to their geographical distributions. The physicochemical characteristic, extraction yields and DPPH scavenging activity of the samples also showed strong link with the sites of their cultivation. The data suggest that geographical distributions affect the levels of phytochemicals and conversely their biological activities. These variations must be taken into consideration while utilizing raw plant materials for industrial applications and traditional therapies.

  17. Assessment of the antibacterial, cytotoxic and antioxidant activities of Morus nigra L. (Moraceae).

    Science.gov (United States)

    Souza, G R; Oliveira-Junior, R G; Diniz, T C; Branco, A; Lima-Saraiva, S R G; Guimarães, A L; Oliveira, A P; Pacheco, A G M; Silva, M G; Moraes-Filho, M O; Costa, M P; Pessoa, C Ó; Almeida, J R G S

    2017-08-17

    This study was carried out to assess the antibacterial, cytotoxic and antioxidant activities of extracts of Morus nigra L. HPLC was used to determine the fingerprint chromatogram of the crude ethanolic extract (Mn-EtOH). The antibacterial effect was assessed through the method of microdilution. The cytotoxicity was tested against human tumour cell lines using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also assessed through the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging and β-carotene-linoleic acid bleaching methods. The presence of phenolic compounds in Mn-EtOH was confirmed using HPLC. The extracts showed activity against most microorganisms tested. The extracts did not show any expressive antiproliferative effect in the assessment of cytotoxicity. The most significant total phenolic content was 153.00 ± 11.34 mg of gallic acid equivalent/g to the ethyl acetate extract (AcOEt). The total flavonoid content was 292.50 ± 70.34 mg of catechin equivalent/g to the AcOEt extract, which presented the best antioxidant activity (IC50 50.40 ± 1.16 μg/mL) for DPPH scavenging. We can conclude that this species shows strong antibacterial and antioxidant activities, as well as weak cytotoxic effects.

  18. Morus nigra leaf extract improves glycemic response and redox profile in the liver of diabetic rats.

    Science.gov (United States)

    Araujo, Carolina Morais; Lúcio, Karine de Pádua; Silva, Marcelo Eustáquio; Isoldi, Mauro César; de Souza, Gustavo Henrique Bianco; Brandão, Geraldo Célio; Schulz, Richard; Costa, Daniela Caldeira

    2015-11-01

    Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia and alterations in the carbohydrate, lipid, and protein metabolism. DM is associated with increased oxidative stress and pancreatic beta cell damage, which impair the production of insulin and the maintenance of normoglycemia. Inhibiting oxidative damage and controlling hyperglycemia are two important strategies for the prevention of diabetes. The pulp and leaf extracts of mulberry (Morus nigra L.) have abundant total phenolics and flavonoids, and its antioxidant potential may be an important factor for modulating oxidative stress induced by diabetes. In this study, DM was induced by intraperitoneal injection of alloxan monohydrate (135 mg kg(-1)). Female Fischer rats were divided into four groups: control, diabetic, diabetic pulp, and diabetic leaf extract. Animals in the diabetic pulp and diabetic leaf extract groups were treated for 30 days with M. nigra L. pulp or leaf extracts, respectively. At the end of treatment, animals were euthanized and, liver and blood samples were collected for analysis of biochemical and metabolic parameters. Our study demonstrated that treatment of diabetic rats with leaf extracts decreased the superoxide dismutase (SOD)/catalase (CAT) ratio and carbonylated protein levels by reducing oxidative stress. Moreover, the leaf extract of M. nigra L. decreased the matrix metalloproteinase (MMP)-2 activity, increased insulinemia, and alleviated hyperglycemia-induced diabetes. In conclusion, our study found that the leaf extract of M. nigra L. improved oxidative stress and complications in diabetic rats, suggesting the utility of this herbal remedy in the prevention and treatment of DM.

  19. A new antioxidant stilbene and other constituents from the stem bark of Morus nigra L.

    Science.gov (United States)

    Abbas, Ghada M; Abdel Bar, Fatma M; Baraka, Hany N; Gohar, Ahmed A; Lahloub, Mohammed-Farid

    2014-01-01

    A new stilbene, 2',3,4',5,5'-pentahydroxy-cis-stilbene (1), along with 13 known compounds, resveratrol (2), oxyresveratrol (3), norartocarpetin (4), kuwanon C (5), morusin (6), cudraflavone A (7), kuwanon G (8), albafurane C (9), mulberrofuran G (10), 3-O-acetyl-α-amyrin (11), 3-O-acetyl-β-amyrin (12) ursolic acid-3-O-acetate (13) and uvaol (14), were isolated from the barks of Morus nigra. Compounds 2, 8, 10, 12 and 14 are reported for the first time from this plant. The isolated compounds were elucidated by means of 1D and 2D NMR, UV, IR and MS, as well as by comparison with the literature data. The isolated compounds and the different extracts were evaluated for their potential antioxidant activity using 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)(+) radical-scavenging capacity assay and compared with ascorbic acid. The new stilbene (1) exhibited remarkable antioxidant capacity with IC50 of 4.69 μM.

  20. Voltammetric determination of antioxidant character in Berberis lycium Royel, Zanthoxylum armatum and Morus nigra Linn plants.

    Science.gov (United States)

    Ahmed, Safeer; Shakeel, Faria

    2012-07-01

    The antioxidant activity potential of three different plant extracts was investigated against superoxide anion radical while employing cyclic voltammetry technique. The plants Berberis lyceum Royle, Morus nigra Linn and Zanthoxylum armatum were selected because of their potential use in the traditional medicine. The voltammetric response of the electrochemically generated superoxide anion radial in DMSO was monitored in the absence and presence of the plat extracts. The decrease in the current was interpreted in terms of antiradical activity of the added extract. The thermodynamic feasibility of the radical scavenging by extracts was accounted in terms of antioxidant activity coefficient (K(ao)) and standard Gibbs free energy (ΔG(o)). The values of K(ao) and ΔG(o) ranged from 1.0 x 102 to 57 x 102 L(-1) and -18 to -27 kJmol(-1), respectively. The possible mechanism of the antioxidant reaction was regarded as E(r)C(i) mechanism i.e. reversible electron transfer followed by hydrogen atom transfer- an irreversible chemical reaction.

  1. Renal biochemical and histopathological alterations of diabetic rats under treatment with hydro alcoholic Morus nigra extrac.

    Science.gov (United States)

    Rahimi-Madiseh, Mohammad; Naimi, Azar; Heydarian, Esfandiar; Rafieian-Kopaei, Mahmoud

    2017-01-01

    Introduction: Morus nigra fruit is known to have antioxidant effects and used to control the blood sugar level in traditional medicine. Objectives: This study was conducted to investigate the biochemical and histopathological changes in the serum and kidneys of diabetic rats treated with hydroalcoholic M. nigra extract. Materials and Methods: In this study, 60 male Wistar rats were divided into five groups of 12 each. After induction of diabetes with alloxan, the diabetic rats were treated with hydroalcoholic extract of M. nigra at different concentrations. Then, the animals were anesthetized and the serum levels of glucose, creatinine, and urea as well as kidney tissue catalase level measured. The kidney tissue was also histopathologically examined. Results: Milder glomerular damage was seen in the group treated with 800 mg/kg of the M. nigra extract compared with diabetic and positive controls, and no difference in the expansion of mesenchymal tissue into renal glomerular vessels observed between the group treated with 800 mg/kg of M. nigra extract and diabetic and positive controls. Furthermore, creatinine levels were significantly higher and urea levels significantly lower in the group treated with 800 mg/kg of M. nigra extract than healthy and positive control groups (Pnigra extract at 800 mg/kg can prevent kidney tissue damage in diabetic rats and this fruit seems to be beneficial to patients with diabetes.

  2. Bioassay-Guided Isolation and Characterization of Wound Healer Compounds from Morus nigra L. (Moraceae

    Directory of Open Access Journals (Sweden)

    Esra Küpeli Akkol

    2015-04-01

    Full Text Available Leaves and fruits of Morus nigra L. (Moraceae are used for the treatment of wounds especially mouth sore in Turkish traditional medicine. The present study was designed to investigate wound healing activity of M. nigra by using incision and excision wound models. Furthermore, anti-inflammatory activity was assessed by Whittle method. Lyophilized fruit extract (MNF displayed significant wound healing activity, while aqueous leaf extract of M. nigra (MNL did not. Through biological activity guided fractionation technique, MNF was subjected to successive solvent extraction. Among the subextracts obtained, n-butanol (MNF-n-BuOH subextract was found to possess wound healing activity. MNF -n-BuOH was subjected Sephadex LH-20 column chromatography to obtain three fractions, which then applied to the same biological activity tests. Compounds 1 and 2 were isolated from the active fraction and their structures were identified as quercetin-3-O-rutinoside and kaempferol-3-O-rutinoside, respectively. The isolates were investigated for their in vitro enzyme inhibitory activities.

  3. Efficacy of Morus nigra L. on reproduction in female Wistar rats.

    Science.gov (United States)

    de Queiroz, Graziela Tonioni; Santos, Tatianne Rosa; Macedo, Renato; Peters, Vera Maria; Leite, Magda Narciso; de Cássia da Silveira e Sá, Rita; de Oliveira Guerra, Martha

    2012-03-01

    Morus nigra L. is a plant employed as a substitute for the conventional hormonal replacement therapy. This work analyzes the estrogenic effect of M. nigra on the reproductive system and embryonic development of Wistar rats. Female rats were orally treated with M. nigra hydroalcoholic extract (MnHE) at the dose levels of 25, 50, 75, 350 and 700 mg/kg of body weight over 15 days, and continued through mating until the 14th day of gestation. Vaginal smears were performed daily and the body weight of the females was recorded at 5 days intervals. On day 15 of gestation, the females were killed and their kidneys, liver, spleen and ovaries were removed and weighed. The number of implants, resorptions, and live and dead fetuses were evaluated. Histological sections of ovaries, measurement of the height of the uterine epithelium and vaginal smears were performed to assess the estrogenic activity. The results showed that the administration of MnHE did not significantly alter the analyzed variables. Therefore, considering the experimental model used in this study, the data obtained indicate that M. nigra did not exhibit any estrogenic activity nor did exert a toxic effect on the female reproductive system and on the embryonic development of rats. Copyright © 2011 Elsevier Ltd. All rights reserved.

  4. Genome-wide identification and expression analyses of cytochrome P450 genes in mulberry (Morus notabilis)

    Institute of Scientific and Technical Information of China (English)

    Bi Ma; Yiwei Luo; Ling Jia; Xiwu Qi; Qiwei Zeng; Zhonghuai Xiang; Ningjia He

    2014-01-01

    Cytochrome P450s play critical roles in the biosyn-thesis of physiological y important compounds in plants. These compounds often act as defense toxins to prevent herbivory. In the present study, a total of 174 P450 genes of mulberry (Morus notabilis C.K.Schn) were identified based on bioinfor-matics analyses. These mulberry P450 genes were divided into nine clans and 47 families and were found to be expressed in a tissue-preferential manner. These genes were compared to the P450 genes in Arabidopsis thaliana. Families CYP80, CYP92, CYP728, CYP733, CYP736, and CYP749 were found to exist in mulberry, and they may play important roles in the biosynthesis of mulberry secondary metabolites. Analyses of the functional and metabolic pathways of these genes indicated that mulberry P450 genes may participate in the metabolism of lipids, other secondary metabolites, xenobiotics, amino acids, cofactors, vitamins, terpenoids, and polyketides. These results provide a foundation for understanding of the structures and biological functions of mulberry P450 genes.

  5. TEST OF Morus khunpai AND M.indica HYBRID TOWARDS SILKWORM FEED

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    Nurhaedah Muin

    2015-09-01

    Full Text Available The sustainability of silkworm rearing depends on the availability of qualified mulberry plants. The research is aimed to observe the compatibility of hybrid Morus khunpai and M.indica (KI hybrid for silkworm's feeding. It is related to the cocoon and filament quality. KI hybrids are a result of pollination control male parent M.khunpai and female parent M.indica S-54. The research applied a Randomized Complete Design by measuring variables, i.e.: the percentage of cocoon, number of normal cocoon, cocoon weight, cocoon shell weight, cocoon shell ratio and filament length. The result showed that cocoon and filament quality of silkworm fed by mulberry hybrid M.khunpai and M.indica (KI have shown non-significant differences compared with silkworm fed by mulberry parents (M.khunpai and M.indica and the control (M.nigra. This situation showed that there has not been recognised yet as the compatibility of hybrid M.khunpai and M.indica (KI hybrid for silkworm feeding.

  6. Genetic diversity and relationships in mulberry (genus Morus as revealed by RAPD and ISSR marker assays

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    Thangavelu K

    2004-01-01

    Full Text Available Abstract Background The genus Morus, known as mulberry, is a dioecious and cross-pollinating plant that is the sole food for the domesticated silkworm, Bombyx mori. Traditional methods using morphological traits for classification are largely unsuccessful in establishing the diversity and relationships among different mulberry species because of environmental influence on traits of interest. As a more robust alternative, PCR based marker assays including RAPD and ISSR were employed to study the genetic diversity and interrelationships among twelve domesticated and three wild mulberry species. Results RAPD analysis using 19 random primers generated 128 discrete markers ranging from 500–3000 bp in size. One-hundred-nineteen of these were polymorphic (92%, with an average of 6.26 markers per primer. Among these were a few putative species-specific amplification products which could be useful for germplasm classification and introgression studies. The ISSR analysis employed six anchored primers, 4 of which generated 93 polymorphic markers with an average of 23.25 markers per primer. Cluster analysis of RAPD and ISSR data using the WINBOOT package to calculate the Dice coefficient resulted into two clusters, one comprising polyploid wild species and the other with domesticated (mostly diploid species. Conclusion These results suggest that RAPD and ISSR markers are useful for mulberry genetic diversity analysis and germplasm characterization, and that putative species-specific markers may be obtained which can be converted to SCARs after further studies.

  7. Improved micropropagation and in vitro fruiting of Morus indica L. (K-2 cultivar

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    Gaurab Gogoi

    2017-06-01

    Full Text Available A rapid economically viable micropropagation protocol has been developed in the present work for Morus indica L. (K-2 cultivar utilizing the readily available nodal explants. Explants were established on different plant growth regulators (PGRs either individually or in combinations. MS medium containing 1 mg L−1 Kinetin (Kin showed the best shoot multiplication with 4.8 ± 0.23 cm average shoot length and 6.5 ± 0.03 number of internodes. Regenerated shoots were elongated in MS medium supplemented with 1.5 mg L−1 gibberellic acid (GA3. Elongated shoots cultured in full-strength MS medium supplemented with 1 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D for one week and then cultured in half-strength MS proved to be more effective in rooting compared to other PGRs in significantly shorter duration. Micropropagated plants transferred to soil fortified with the quarter-strength of MS salts along with humidity regulation process showed 89% survival frequency. In vitro flowering in the regenerated shoots was also observed in the MS medium supplemented with (1.5 mg L−1 Kin and carbon source replaced by commercial sugar cubes. This method can be effectively used for in vitro culture of M. indica in commercial scale owing to its enhanced quality and reduced time frame.

  8. Acaricidal activity of leaves of Morus nigra against the cattle tick Rhipicephalus microplus

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    A.C.S. Dantas

    Full Text Available ABSTRACT The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of eggs eclosion average and 65.4% of effectiveness. HPLC analysis revealed the presence of phenolic compounds, which may be related to biological activity shown by the extracts, which can be used as an alternative control against R. microplus adult tick.

  9. Fosfato de sódio e cloreto de potássio na micropropagação de videira e amoreira-preta = Sodium phosphate and potassium chloride concentrations: micropropagation of grapevine and blackberry

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    Fabíola Villa

    2007-10-01

    Full Text Available Objetivou-se no presente trabalho testar diferentes concentrações de fosfato de sódio (NaH2PO4.H2O e cloreto de potássio (KCl do meio White e adicionadas ao meio Knudson, na multiplicação in vitro de videira e amoreira-preta. O meio foi constituído de sais doKnudson, acrescido de 25 g L-1 de sacarose e 6 g L-1 de ágar, e o pH ajustado para 5,8 antes da autoclavagem a 121ºC e 1 atm por 20 minutos. Os tratamentos consistiram de concentrações de NaH2PO4.H2O (0, 125, 250, 500 e 1.000 mg L-1 e de KCl (0, 125, 250, 500 e 1.000 mg L-1, em todas as combinações possíveis e do porta-enxerto de videira ‘R110’ e da amoreira-preta cv. Cherokee. Segmentos nodais, oriundos de plântulas preestabelecidas in vitro foram excisados eintroduzidos em tubos de ensaio contendo 15 mL do meio de cultura. Posteriormente, os tubos de ensaio foram transferidos para sala de crescimento a 25 ± 2ºC, irradiância de 32 mmol m–2 s–1 efotoperíodo de 16 horas diárias. O delineamento experimental foi inteiramente casualisado, utilizando-se 4 repetições com 12 plântulas cada. O experimento foi avaliado após 60 dias de cultivo in vitro e o maior comprimento da parte aérea e peso fresco de calos foram verificados em meio Knudson, na ausência de cloreto de potássio. A adição de cloreto de potássio ao meio Knudson não influenciou no aumento do comprimento da parte aérea das duas frutíferas e namassa fresca de calos do porta-enxerto de videira ‘R110’. Não é necessário adicionar fosfato de sódio ao meio para se obter maior massa fresca da parte aérea das frutíferas.This work aimed to test different concentrations of sodium phosphate (NaH2PO4.H2O and potassium chloride (KCl taken from the White medium and added to the Knudson culture medium, in the in vitro multiplication of grapevine rootstocks and blackberry. The culture medium was constituted of Knudson salts, added of 25 g L-1 of sucrose and 6 g L-1 of agar, and the adjusted pH to 5

  10. The crystals of a mannose-specific jacalin-related lectin from Morus nigra are merohedrally twinned.

    Science.gov (United States)

    Rabijns, A; Verboven, C; Novoa de Armas, H; Van Damme, E J; Peumans, W J; De Ranter, C J

    2001-04-01

    MornigaM, a lectin from Morus nigra, belongs to the mannose-binding subgroup of the family of jacalin-related plant lectins. It was crystallized in the P6(5) space group, with unit-cell parameters a = b = 110.74, c = 159.28 A. The partially merohedrally twinned crystals could be detwinned and a subsequent molecular-replacement solution could be found using the coordinates of jacalin. Preliminary analysis clearly shows the tetrameric assembly of this protein. Furthermore, data from MornigaM crystals soaked in a mannose solution were collected.

  11. Female barn owls (Tyto alba) advertise good genes.

    Science.gov (United States)

    Roulin, A; Jungi, T W; Pfister, H; Dijkstra, C

    2000-05-07

    The good genes hypothesis of sexual selection postulates that ornamentation signals superior genetic quality to potential mates. Support for this hypothesis comes from studies on male ornamentation only, while it remains to be shown that female ornamentation may signal genetic quality as well. Female barn owls (Tyto alba) display more black spots on their plumage than males. The expression of this plumage trait has a genetic basis and it has been suggested that males prefer to mate with females displaying more black spots. Given the role of parasites in the evolution of sexually selected traits and of the immune system in parasite resistance, we hypothesize that the extent of female plumage 'spottiness' reflects immunological defence. We assessed the genetic variation in specific antibody production against a non-pathogenic antigen among cross-fostered nestlings and studied its covariation with the plumage spottiness of genetic parents. The magnitude of the antibody response was positively correlated with the plumage spottiness of the genetic mother but not of the genetic father. Our study thereby provides the first experimental support, to our knowledge, for the hypothesis that female ornamentation signals genetic quality.

  12. Memory-enhancing activity of Rose alba in mice

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    Naikwade Nilofar

    2009-01-01

    Full Text Available Alzheimer′s disease (AD is a neurodegenerative disorder currently without an effective treatment. Impairment of memory is the initial and most significant symptom of AD. Memantine is the first novel class of AD medications acting on the glutaminergic system and produces symptomatic improvement in learning. Nootropic agents such as piracetam, aniracetam, and choline esterase inhibitors like donepezil are being used to improve memory, mood, and behavior, but the resulting side-effects associated with these agents have made their use limited. The present study was undertaken to investigate the effects of Rose alba (RA on learning and memory in mice. Male Swiss albino mice (3 months old weighing around 25 g were employed in the present investigation. Elevated plus-maze and passive-avoidance apparatus served as the exteroceptive behavioral models, and diazepam-induced amnesia served as the interoceptive behavioral models. RA (100 and 200 mg/kg p.o. was administered for eight successive days to the mice. Piracetam (200 mg/kg i.p. was used as a standard nootropic agent. RA improved learning and memory of mice as indicated by decreased transfer-latency and increased step-down latency. RA significantly reversed the amnesia induced by diazepam (1 mg/kg, i.p.. The results indicate that the aqueous extract of calyces of RA might prove to be a useful memory restorative agent in the treatment of cognitive disorders.

  13. Nanometer accuracy with continuous scans at the ALBA-NOM

    Science.gov (United States)

    Nicolas, Josep; Pedreira, Pablo; Å ics, Igors; Ramírez, Claudio; Campos, Juan

    2016-09-01

    We present the continuous scan operation of the ALBA-NOM as a working mode that allows obtaining low noise in short time, as well as high accuracy measurements. In the traditional step-scan operation, the position of the probe beam is kept fixed while many data points of autocollimator are averaged for noise reduction. This operation mode is very safe, as one has a perfect correspondence between mirror position and measured angle, but it is time inefficient, as it disregards all the data values acquired during motion, and basically averages data values taken under identical conditions. On the other hand, continuous scan is less safe in terms of correspondence between mirror position and slope, especially for NOM systems for which the autocollimator does not accept an electronic trigger. Nevertheless, it is possible to perform independent acquisitions of the autocollimator and of the linear stage data during a scan, and synchronize signals a posteriori. This solves the main problem of continuous scan with a NOM. Continuous scan operation for performing measurements is very efficient for noise reduction per unit time, as it allows integrating every single data value taken by the autocollimator. In addition, it opens the possibility of introducing pitch variations of the mirror between scans. This allows obtaining many independent datasets that can be combined using error suppression techniques to reduce not just noise but systematic errors too. In this paper we report the methods and the main results.

  14. SimAlba: a Spatial Microsimulation approach to the analysis of health inequalities

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    Malcolm Campbell

    2016-10-01

    Full Text Available This paper presents applied geographical research based on a Spatial Microsimulation model, SimAlba aimed at estimating geographically sensitive health variables in Scotland. SimAlba has been developed in order to answer a variety of 'what-if' policy questions pertaining to health policy in Scotland. Using the SimAlba model, it is possible to simulate the distributions of previously unknown variables at the small area level such as; smoking, alcohol consumption, mental well-being and obesity. The SimAlba microdataset has been created by combining Scottish Health Survey (SHS and Census data using a deterministic reweighting Spatial Microsimulation algorithm developed for this purpose. The paper presents SimAlba outputs for Scotland’s largest city, Glasgow and examines the spatial distribution of the simulated variables for small geographical areas in Glasgow as well as the effects on individuals of different policy scenario outcomes. In simulating previously unknown spatial data a wealth of new perspectives can be examined and explored. This paper explores a small set of those potential avenues of research and shows the power of spatial microsimulation modelling in an urban context.

  15. SimAlba: A Spatial Microsimulation Approach to the Analysis of Health Inequalities.

    Science.gov (United States)

    Campbell, Malcolm; Ballas, Dimitris

    2016-01-01

    This paper presents applied geographical research based on a spatial microsimulation model, SimAlba, aimed at estimating geographically sensitive health variables in Scotland. SimAlba has been developed in order to answer a variety of "what-if" policy questions pertaining to health policy in Scotland. Using the SimAlba model, it is possible to simulate the distributions of previously unknown variables at the small area level such as smoking, alcohol consumption, mental well-being, and obesity. The SimAlba microdataset has been created by combining Scottish Health Survey and Census data using a deterministic reweighting spatial microsimulation algorithm developed for this purpose. The paper presents SimAlba outputs for Scotland's largest city, Glasgow, and examines the spatial distribution of the simulated variables for small geographical areas in Glasgow as well as the effects on individuals of different policy scenario outcomes. In simulating previously unknown spatial data, a wealth of new perspectives can be examined and explored. This paper explores a small set of those potential avenues of research and shows the power of spatial microsimulation modeling in an urban context.

  16. EFFECT OF TEMPERATURE ON THE BIOLOGY OF TUBEROLACHNUS SALIGNUS (GMELIN (STERNORRHYNCHA: APHIDIDAE ON (SALIX ALBA

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    Nıhal ÖZDER

    2008-07-01

    Full Text Available The development time, survivoship and reproduction of Tuberolachnus salignus (Gmelin( Lachninae: Lachnini were studied on Salix alba at fi ve constant temperatures (17.5°C, 20°C, 22.5°C, 25°C and 27.5°C . The developmental time of immature stages ranged from 17.00 days at 17.5°C to 12.21 days at 25°C on Salix alba. The total percentage of survivorship of immature stages varied from 50% and 70% 17.5°C -20°C on S. alba. The largest r m valueoccurred with 0.2540 at 20°C on S. alba. The mean generation time of the population ranged from 13.595 days at 22.5°C to 19.60 days at 17.5°C on S. alba. The optimal temperature for Tuberolachnus salignus was 20°C.

  17. AN EFFECTIVE WAY TO CARRY OUT MASS IN VITRO PROPAGATION OF POTENTILLA ALBA L.

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    L. I. Tikhomirova

    2016-04-01

    Full Text Available Despite the plant’s extensive area of distribution, Potentilla alba L. natural resources are scarce and cannot meet the modern needs of the pharmaceutical industry. Because of the mass preparation of medical raw materials by using P. alba, it entered into the list of rare and endangered species plants of the Red Data Book of the Republic of Belarus. This plant is not represented in the wild flora of Western Siberia, but there is a great need for developing a method for the mass propagation of P. alba using in vitro culture in order to obtain a high-quality planting material. At the explant stage, the technique of the P. alba introduction into in vitro culture is developed. This paper reveals the morphogenetic features of the development of P. alba explants of different types and the regenerative capacity of the tissue culture. At the micropropagation stage, the optimum culture media and the growth conditions for the regenerated plants are selected. At the stage of test-tube plants rooting and transferring them into ex vitro conditions, the most effective means of adaptation to non-sterile conditions in hydroponics are proposed.

  18. Molluscicidal activity of Morus nigra against the freshwater snail Lymnaea acuminata

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    Farheen Hanif

    2012-01-01

    Full Text Available The molluscicidal activity of Morus nigra fruit, bark and leaf powder against the snail Lymnaea acuminata was time and concentration dependent. Toxicity of fruit powder (96h LC50: 166.92 mg/L was more pronounced in comparison to bark powder (96h LC50: 173.17 mg/L and leaf powder (96h LC50: 173.69 mg/L. Ethanolic extracts of M. nigra fruit, bark and leaf was more toxic than their other organic solvent extracts. The molluscicidal activity of ethanolic extract of M. nigra fruit powder (24h LC50: 116.23 mg/L was more effective than the ethanolic extract of bark powder (24h LC50: 154.41 mg/L and leaf powder (24h LC50: 139.80 mg/L. The 96h LC50 of column-purified fraction of M. nigra fruit powder was, 10.03 mg/L whereas that of bark and leaf powder was 8.69 mg/L and 4.97 mg/L, respectively. Column and thin layer chromatography analysis demonstrates that the active molluscicidal component in M. nigra is quercetin (96h LC50: 1.11 mg/L, apigenin (96h LC50: 1.92 mg/L and morusin (96h LC50: 2.12 mg/L, respectively. Co-migration of quercetin (Rf 0.49, apigenin (Rf 0.51 and morusin (Rf 0.52 with column-purified fruit, bark and leaf of M. nigra on thin layer chromatography demonstrates same Rf value. The present study indicates that M. nigra may be used as potent source of molluscicides against the snail Lymnaea acuminata.

  19. Effects of Flavonoids in Morus indica on Blood Lipids and Glucose in Hyperlipidemia-diabetic Rats

    Institute of Scientific and Technical Information of China (English)

    MA Dong-qing; JIANG Zheng-ju; XU Shi-qiang; YU Xing; HU Xia-min; PAN Hong-yan

    2012-01-01

    Objective To investigate the effect and possible mechanism of flavonoids extracted from Morus indica(FMI)on blood lipids and glucose.Methods The experimental hyperlipidemia-diabetic rats were induced by high-fat diet(HFD)and low dose of Streptozotocin(STZ).Flavonoids-treated rats were pretreated with FMI(50,100,and 200 mg/kg).The plasma,skeletal muscle,and livers were isolated for biochemical assays,HE staining,immunohistochemistry,and Western blotting analysis.Results The results showed that the body weight in flavonoidstreated(100 and 200 mg/kg)rats was reduced(P<0.05,0.01)compared to HFD-fed rats.FMI obviously reduced total cholesterol(P<0.01),triglycerides(P<0.05),and low-density lipoprotein cholesterol(P<0.05),increased high-density lipoprotein(P<0.05),and significantly decreased the atherosclerosis index(P<0.01);FMI(100 and 200 mg/kg)also down-regulated the elevation of blood glucose induced by STZ(P<0.05,0.01);At the meantime,FMI increased hepatic superoxide dismutase(SOD)activity and reduced hepatic malondialdehyde(MDA)content obviously.In addition,the results showed that the expression of hepatic CYP2E1 was markedly decreased while the expression of GLUT-4 in skeletal muscles was increased by FMI.Conclusion The above results demonstrate that the effect of FMI is related to its up-regulation of hepatic SOD activity,reduction of hepatic MDA content,down-regulation of hepatic CYP2E1 expression,and increase of GLUT-4 expression in skeletal muscle,which suggests that FMI may prevent or improve hyperlipidemia and hyperglycemia caused by an excessive HFD.

  20. Gastroprotective Value of Berries: Evidences from Methanolic Extracts of Morus nigra and Rubus niveus Fruits

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    Luciane Angela Nottar Nesello

    2017-01-01

    Full Text Available This study evaluated the gastroprotective value of the methanol extracts from fruits of Morus nigra L. (black mulberry (MEMN and Rubus niveus Thunb (raspberry (MERN. The total phenolic compounds and flavonoids were measured, as well as the in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH free radical scavenger activity. The gastroprotective effects of the extracts against 60% ethanol/0.3 M HCl were evaluated in mice. After that, the lipid hydroperoxides and reduced glutathione levels at ulcerated tissue were determined. The effects of extracts on H+/K+-ATPase activity were also verified. The extracts exhibited high contents of polyphenols; however, MERN presented 1.5-fold higher levels. The presence of flavonoids also was confirmed. In addition, MEMN (IC50 = 13.74 μg/mL and MERN (IC50 = 14.97 μg/mL scavenged DPPH radical. The MEMN reduced the ulcer area only at 300 mg/kg (p.o. by 64.06%. Interestingly, MERN decreased the ulcer area in a superior potency (ED50 = 20.88 mg/kg, reducing the ulcer area by 81.86% at 300 mg/kg, and increased the gastric mucin levels. The antioxidant effects of extracts were evidenced by reduced lipoperoxides and increased reduction of glutathione amount in the gastric mucosa. However, MEMN or MERN did not change the H+/K+-ATPase activity. These results confirm that M. nigra and R. niveus are berries with a gastroprotective value by strengthening of gastric protective factors.

  1. Morus nigra and its major phenolic, syringic acid, have antidepressant-like and neuroprotective effects in mice.

    Science.gov (United States)

    Dalmagro, Ana Paula; Camargo, Anderson; Zeni, Ana Lúcia Bertarello

    2017-08-18

    Depression is a disorder with a high incidence that has been increasing worldwide although the pathophysiology remains unclear. Moreover, some studies revealed a higher concentration of glutamate and oxidative stress in the patients' brain, which causes cell death by excitotoxicity. Morus nigra L. is known as black mulberry and its leaves are popularly used to treat affections related to menopause, obesity and high cholesterol. M. nigra leaves are a rich fount of phenolics which well-known by the antioxidant property. Herein, we examined the phenolic profile and the antidepressant-like effect of the Morus nigra aqueous extract (MN) and its major phenolic constituent, syringic acid (SA). Furthermore, the involvement of antioxidant and neuroprotective activities were further evaluated. Our results show that acute and subchronic MN or SA administration exerted antidepressant-like property in the behavioral testes in mice. The results suggest that the antidepressant-like effect of MN, at least in part, could be due to the SA influence. Moreover, the observed effect involves the nitro-oxidative system modulation in both the serum and brain of mice. Furthermore, MN or SA was able to contain the glutamate-induced cell death in the hippocampal and cortical slices implicating the neuroprotection activity in the antidepressant-like effect.

  2. Annotation and Re-Sequencing of Genes from De Novo Transcriptome Assembly of Abies alba (Pinaceae

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    Anna M. Roschanski

    2013-01-01

    Full Text Available Premise of the study: We present a protocol for the annotation of transcriptome sequence data and the identification of candidate genes therein using the example of the nonmodel conifer Abies alba. Methods and Results: A normalized cDNA library was built from an A. alba seedling. The sequencing on a 454 platform yielded more than 1.5 million reads that were de novo assembled into 25 149 contigs. Two complementary approaches were applied to annotate gene fragments that code for (1 well-known proteins and (2 proteins that are potentially adaptively relevant. Primer development and testing yielded 88 amplicons that could successfully be resequenced from genomic DNA. Conclusions: The annotation workflow offers an efficient way to identify potential adaptively relevant genes from the large quantity of transcriptome sequence data. The primer set presented should be prioritized for single-nucleotide polymorphism detection in adaptively relevant genes in A. alba.

  3. PHYTOCHEMICAL CHARACTERISATION AND PHARMACOLOGICAL EVALUATION OF METHANOLIC EXTRACT OF AVICENNIA ALBA

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    Sandip Kr. Baidya et al

    2012-10-01

    Full Text Available The history of herbal medicines is as old as civilization. For this purpose of ascertaining the phytochemical constituents, the present work was performed into the phytochemical investigation of the plant Avicenna alba. Separation and isolation of the constituents of interest, characterization of isolated compounds and investigation of the biosynthetic pathways of the particular pathway and quantitative evaluation. Another reason for choosing this plant is the availability of the plant. Avicenna alba is the important plant used from the past. It was reported to show antimicrobial activity in literature survey. So now, the time has come to perform a proper phytochemical investigation to find its proper way into medicine. Our plan of work is to identify and isolate the chemical constituents of the aerial part of Avicennia alba and to evaluate its pharmacological action. With the above aim, this experimental work was undertaken.

  4. Flour from Prosopis alba cotyledons: A natural source of nutrient and bioactive phytochemicals.

    Science.gov (United States)

    Cattaneo, F; Costamagna, M S; Zampini, I C; Sayago, J; Alberto, M R; Chamorro, V; Pazos, A; Thomas-Valdés, S; Schmeda-Hirschmann, G; Isla, M I

    2016-10-01

    The Prosopis alba seed is a waste material in the process to produce pod flour. To suggest a potential use of these seeds it is necessary to determine the nutritional, phytochemical and functional quality of cotyledon flour from Prosopis alba. This flour showed high level of proteins (62%), low content of total carbohydrate and fat. Free polyphenol (1150±20mg GAE/100g flour) and carotenoids (10.55±0.05mg β-CE/100g flour) compounds were the dominant compounds. The main identified constituents in the polyphenolic extracts were C- glycosyl flavones, including schaftoside, isoschaftoside, vicenin II, vitexin and isovitexin. The extract enriched in polyphenolic compounds exhibited ABTS(+) reducing capacity and scavenging activity of H2O2; and was able to inhibit phospholipase, lipoxygenase and cyclooxygenase, three pro-inflammatory enzymes. According to our results, the P. alba cotyledon flour could be considered as a new alternative in the formulation of functional foods or food supplements.

  5. Identification and evaluation of anti Hepatitis C Virus phytochemicals from Eclipta alba

    Science.gov (United States)

    Manvar, Dinesh; Mishra, Mahesh; Kumar, Suriender; Pandey, Virendra N.

    2012-01-01

    Ethnopharmacological relevance Eclipta alba, traditionally known as bhringraj, has been used in Ayurvedic medicine for more than 1000 years in India. It is used for the treatment of infective hepatitis, liver cirrhosis, liver enlargement and other ailments of liver and gall bladder in India. Aim of the study To evaluate anti-hepatitis C virus activity present in the Eclipta alba extract, perform bioassay based fractionation and identify anti-HCV phytochemicals from the active fractions. Materials and methods Identification of active compounds was performed by bio-activity guided fractionation approach. Active isolates were separated by the combination of silica gel chromatography and preparative scale reverse phase HPLC. Eclipta alba extract and its isolates were examined for their ability to inhibit HCV replicase (HCV NS5B) activity in vitro and HCV replication in a cell culture system carrying replicating HCV subgenomic RNA replicon. The purified isolates were also examined for their binding affinity to HCV replicase by fluorescence quenching and their cytotoxicity by MTT assay. Results Eclipta alba extract strongly inhibited RNA dependent RNA polymerase (RdRp) activity of HCV replicase in vitro. In cell culture system, it effectively inhibited HCV replication which resulted in reduced HCV RNA titer and translation level of viral proteins. Bioassay-based fractionations of the extracts and purification of anti-HCV phytochemicals present in the active fractions have identified three compounds, wedelolactone, luteolin, and apigenin. These compounds exhibited dose dependent inhibition of HCV replicase in vitro, and anti-HCV replication activity in the cell culture system Conclusion Eclipta alba extract and phytochemicals isolated from active fractions display anti-HCV activity in vitro and in cell culture system. The standardized Eclipta alba extract or its isolates can be used as an effective alternative and complementary treatment against HCV. PMID:23026306

  6. Presencia de hifas de Evernia prunastri (L.) Ach. en los tejidos caulinares de Betula alba L

    OpenAIRE

    Monsó Senabre, María Andrea; Solas Alados, María Teresa; Fernández Ruiz, Benjamín

    1992-01-01

    [ES] En el presente trabajo se estudia el mecanismo y alcance de la penetración de las hifas de un liquen epífito, Evernia prunastri (L.) Ach., en ramas de Betula alba L. Se pretende averiguar la relación entre el ataque químico de las substancias liquénicas con el metabolismo foliar. [EN] The present research describes the results of the mechanisms and reach penetration study of an epiphytic lichen, Evernia prunastri (L.) Ach. into the branches of Betula alba L. It's a question of to disc...

  7. Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonas sp.

    OpenAIRE

    FERNANDO J. SUTILI; Cunha,Mauro A.; Rosangela E. Ziech; Krewer,Carina C.; CARLA C. ZEPPENFELD; Heldwein, Clarissa G.; Gressler, Leticia T.; Berta M. Heinzmann; Agueda C. de Vargas; BERNARDO BALDISSEROTTO

    2015-01-01

    In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonas sp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1 for ...

  8. Venezuela e ALBA: regionalismo contra-hegemônico e ensino superior para todos Venezuela and the ALBA: counter-hegemonic regionalism and higher education for all

    Directory of Open Access Journals (Sweden)

    Thomas Muhr

    2010-08-01

    Full Text Available Partindo de um quadro teórico neo-gramsciano crítico à globalização, este artigo aplica a nova teoria do regionalismo (NTR e a teoria do regionalismo regulatório (TRR à sua análise e teorização dos tratados de comércio da Aliança Bolivariana para os Povos da Nossa América (ALBA-TCP como regionalismo contra-hegemônico na América Latina e Caribe (ALC. A ALBA está centrada na ideia de um Socialismo do Século XXI, que, como (inicialmente também a Revolução Bolivariana da Venezuela, substitui a 'vantagem competitiva' pela 'vantagem cooperativa'. Em seu caráter de conjunto de processos multidimensionais e transnacionais a ALBA-TCP opera dentro de/transversalmente a um número de setores e escalas, ao mesmo passo que as transformações estruturais são movidas pela interação de agentes do Estado e agentes não estatais. A política de Educação Superior para Todos (ESPT do governo venezuelano rejeita a agenda neoliberal globalizada de mercadorização, privatização e elitismo e reinvindica educação pública gratuita em todos os níveis como um direito humano fundamental. A ESPT está sendo regionalizado em um espaço educacional emergente da ALBA e assume um papel-chave nos processos de democracia direta e participatória, dos quais a construção popular (bottom-up da contra-hegemonia e a redefinição política e econômica da ALC dependem. Antes de produzir sujeitos empreendedores conformes ao capitalismo global, a ESPT procura formar subjetividades ao longo de valores morais de solidariedade e cooperação. Isso será ilustrado com referência a um estudo etnográfico de caso da Universidade Bolivariana da Venezuela (UBV.This paper employs new regionalism theory and regulatory regionalism theory in its analysis and theorisation of the Bolivarian Alliance for the Peoples of Our America (ALBA as a counter-hegemonic Latin American and Caribbean (LAC regionalism. As (initially the regionalisation of Venezuela's Bolivarian

  9. LA PALABRA DIBUJADA. ANTONIO FERNÁNDEZ-ALBA, PRIMER Y ÚLTIMO MAESTRO / The drawn word. Antonio Fernández-Alba, first and last master

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    Juan Luis Trillo de Leyva

    2015-05-01

    Full Text Available Távora y Fernández-Alba son figuras de referencia ineludible para quienes quieran hoy investigar el origen contemporáneo de la arquitectura peninsular y, sobre todo, de sus escuelas. Alba fue siempre una guía para los que abordamos una enseñanza alejados del aura que acompañaba a los grandes arquitectos madrileños y barceloneses. Su experimentación didáctica alteró la trayectoria de las escuelas de arquitectura españolas. Introdujo la cultura contemporánea en la universidad como si se tratara de una segunda naturaleza, de un nuevo estrato territorial de los proyectos, manteniendo al hombre como objeto central y destinatario de todo proceso proyectual. En época de virtualidad y representación automatizada, nada más adecuado que la revisión de los dibujos del maestro Fernández-Alba. Dibujos que requieren ser proyectados, pensados, antes que ejecutados, “proyectos de proyectos”, una especie de tautología que rige la mente del poeta en su continua reducción, compresión o destilación, gota a gota, del mundo real, del universo y la palabra.

  10. Alcoholic Extract of Eclipta alba Shows In Vitro Antioxidant and Anticancer Activity without Exhibiting Toxicological Effects

    Science.gov (United States)

    Arya, Rakesh Kumar; Dev, Kapil; Sharma, Chetan; Hossain, Zakir; Meena, Sanjeev; Arya, K. R.; Gayen, J. R.

    2017-01-01

    As per WHO estimates, 80% of people around the world use medicinal plants for the cure and prevention of various diseases including cancer owing to their easy availability and cost effectiveness. Eclipta alba has long been used in Ayurveda to treat liver diseases, eye ailments, and hair related disorders. The promising medicinal value of E. alba prompted us to study the antioxidant, nontoxic, and anticancer potential of its alcoholic extract. In the current study, we evaluated the in vitro cytotoxic and antioxidant effect of the alcoholic extract of Eclipta alba (AEEA) in multiple cancer cell lines along with control. We have also evaluated its effect on different in vivo toxicity parameters. Here, we found that AEEA was found to be most active in most of the cancer cell lines but it significantly induced apoptosis in human breast cancer cell lines by disrupting mitochondrial membrane potential and DNA damage. Moreover, AEEA treatment inhibited migration in both MCF 7 and MDA-MB-231 cells in a dose dependent manner. Further, AEEA possesses robust in vitro antioxidant activity along with high total phenolic and flavonoid contents. In summary, our results indicate that Eclipta alba has enormous potential in complementary and alternative medicine for the treatment of cancer. PMID:28250894

  11. Cytotoxic and Antimicrobial Constituents from the Essential Oil of Lippia alba (Verbenaceae

    Directory of Open Access Journals (Sweden)

    Nara O. dos Santos

    2016-08-01

    Full Text Available Backgroud: Lippia alba (Verbenaceae is a plant widely used in folk medicine to treat various diseases. The present work deals with the chemical composition of the crude essential oil extracted from leaves of L. alba and evaluation of its antimicrobial and cytotoxic activities. Methods: Leaves of L. alba were extracted by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS as well as by nuclear magnetic resonance (NMR spectroscopy. Cytotoxic and antimicrobial activities of crude essential oil were evaluated in vitro using MTT and broth microdilution assays, respectively. Results: Chemical analysis afforded the identification of 39 substances corresponding to 99.45% of the total oil composition. Concerning the main compounds, monoterpenes nerol/geraniol and citral correspond to approximately 50% of crude oil. The cytotoxic activity of obtained essential oil against several tumor cell lines showed IC50 values ranging from 45 to 64 µg/mL for B16F10Nex2 (murine melanoma and A549 (human lung adenocarcinoma. In the antimicrobial assay, was observed that all tested yeast strains, except C. albicans, were sensitive to crude essential oil. MIC values were two to four-folds lower than those determined to bacterial strains. Conclusion: Analysis of chemical composition of essential oils from leaves of L. alba suggested a new chemotype nerol/geraniol and citral. Based in biological evidences, a possible application for studied oil as an antifungal in medicine, as well as in agriculture, is described.

  12. Temporal variability in the Abra alba community determined by global and local events

    NARCIS (Netherlands)

    Van Hoey, V.H.; Vincx, M.; Degraer, S.

    2007-01-01

    Macrobenthic communities in temperate, shallow coastal waters are characterised by strong seasonal and year-to-year variations in community characteristics. These temporal variations were investigated in the Abra alba community on the Belgian Continental Shelf over a period of nine years (1995 – 200

  13. Hepatoprotective activity of Eclipta alba against carbon tetrachloride-induced hepatotoxicity in albino rats

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    Ravindra S. Beedimani

    2015-06-01

    Conclusion: The results of the study confirmed the hepatoprotective activity of aqueous extracts of E. alba at doses of 250 mg/kg and 500 mg/kg against CCl4 induced hepatotoxicity in rats. However, the dose adjustments may be necessary to optimize the similar hepatoprotective efficacy in clinical settings. [Int J Basic Clin Pharmacol 2015; 4(3.000: 404-409

  14. Una receta de tinta de escritura procedente del Archivo de la Casa de Alba

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    Teresa María Criado Vega

    2015-12-01

    Full Text Available Archive of the house of Alba keeps a file on your prescription black ink mode of metalogalics. Throughout this work, we have studied the materials and processes as well as Castilian been collated with several recipes, and coeval with the same theme, as reflected in various Castilian recipes deposited on different backgrounds.

  15. [Genetic control of Silver fir isozymes (Abies alba Mill.) of the Ukrainian Carpathian Mountains].

    Science.gov (United States)

    Korshikov, I I; Morozova, N N; Pirko, Ia V

    2003-01-01

    Genetic control of GOT, GDH, DIA, MDH, ME, SOD, FDH, ADH, ACP, LAP enzymes has been studied in the seed megagametophytes of Silver fir (Abies alba Mill.) from four natural populations of the Ukrainian Carpathian mountains. The distinct electrophoretic division has been obtained for the 21 loci products. The analysis of allele segregation in the heterozygous trees confirms monogenic inheritance of the revealed variants.

  16. Study of genetic polymorphism of Artemisia herba-alba from Tunisia ...

    African Journals Online (AJOL)

    SERVER

    2008-01-04

    Jan 4, 2008 ... limitation with the use of such molecular markers in A. herba-alba is its ... for each locus using the polymorphism information content (PIC). (Smith et al. .... monomorphic patterns because no mutations are located in target sites ...

  17. Chemical characterization of Lippia alba essential oil: an alternative to control green molds

    Directory of Open Access Journals (Sweden)

    Jasmina Glamočlija

    2011-12-01

    Full Text Available The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by ¹H and 13C-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC and minimum fungicidal concentration (MFC. Commercial fungicides Ketoconazole and Bifonazole were used as control. Essential oil yield is of 0.15% and the major components are neral (33.32% and geranial (50.94%. The L. alba essential oil has MIC of 0.300-1.250 mg/mL and MFC of 0.600-1.250 mg/mL. Ketoconazole and Bifonazole show MIC ranging from 0.025-0.500 to 0.100-0.200 mg/mL, and MFC ranging from 0.250-0.100 to 0.200-0.250 mg/mL, respectively. L. alba essential oil is classified as citral type and the results indicate that it is a potential alternative to synthetic fungicides.

  18. PfAlba1: master regulator of translation in the malaria parasite.

    Science.gov (United States)

    Bunnik, Evelien M; Le Roch, Karine G

    2015-10-08

    During the asexual replication cycle of the malaria parasite Plasmodium falciparum, the RNA-binding protein PfAlba1 binds and stabilizes a subset of transcripts for translation at a later time point.Please see related Research article: http://www.genomebiology.com/2015/16/1/212.

  19. Chemical characterization of Lippia alba essential oil: an alternative to control green molds.

    Science.gov (United States)

    Glamočlija, Jasmina; Soković, Marina; Tešević, Vele; Linde, Giani Andrea; Colauto, Nelson Barros

    2011-10-01

    The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by (1)H and (13)C-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Commercial fungicides Ketoconazole and Bifonazole were used as control. Essential oil yield is of 0.15% and the major components are neral (33.32%) and geranial (50.94%). The L. alba essential oil has MIC of 0.300-1.250 mg/mL and MFC of 0.600-1.250 mg/mL. Ketoconazole and Bifonazole show MIC ranging from 0.025-0.500 to 0.100-0.200 mg/mL, and MFC ranging from 0.250-0.100 to 0.200-0.250 mg/mL, respectively. L. alba essential oil is classified as citral type and the results indicate that it is a potential alternative to synthetic fungicides.

  20. Influência do meio de cultura, tipo e concentração de citocininas na multiplicação in vitro de amoreira-preta e framboeseira The influence of culture medium, cytokinin type and concentrations on in vitro multiplication of blackberry and raspberry

    Directory of Open Access Journals (Sweden)

    Luciene Nolasco Leitzke

    2010-04-01

    Full Text Available A amoreira-preta e a framboeseira são espécies pouco cultivadas no Brasil, porém representam uma ótima opção de diversificação para pequenas propriedades, por serem rústicas e com elevada produtividade. A propagação dessas espécies dá-se, principalmente, por meio de estacas de raiz e mesmo de hastes novas, porém, já é crescente o interesse pelo uso da micropropagação como um método alternativo de propagação vegetativa de plantas frutíferas. No entanto, existe a necessidade de se ajustar, para cada espécie, e/ou cultivar, as melhores condições de cultivo, para que se obtenha sucesso no processo. Assim, neste trabalho objetivou-se determinar o melhor meio de cultura (MS e WPM, o efeito das citocininas (BAP, 2iP, Zeatina e suas concentrações (0; 7,5 ;15 e 22,5 μM na multiplicação in vitro de amoreira-preta 'Xavante' e de framboeseira 'Batum' e 'Heritage'. De acordo com os resultados obtidos, conclui-se que, o meio MS e a citocinina BAP na concentração de 15 μM foi mais eficiente para ambas as espécies e cultivares, induzindo maior número médio de folhas, brotações e gemas. Quanto ao comprimento das brotações, observou-se um comportamento diferenciado entre espécie, cultivar e regulador. Para amoreira-preta, o cultivo em meio MS na ausência de regulador promoveu o aumento do comprimento das brotações, enquanto que para framboeseira 'Batum' e 'Heritage', melhores resultados foram obtidos na presença de citocininas, respectivamente, Zeatina e 2iP, independentemente das concentrações utilizadas.The blackberry and raspberry are species little cultivated in Brazil. However, they represent a valuable diversification option for small farms, once they are rustic and very productive. Root cuttings and softwood stem are the most widely practiced type of propagation. Recently, there has been an increased interest in the use of micropropagation as an alternative technique for fruit plant propagation. Nevertheless

  1. Enraizamento de estacas caulinares e radiculares de cultivares de amoreira-preta coletadas em diferentes épocas, armazenadas a frio e tratadas com AIB Rooting of stems and root cutting of blackberry cultivars collected in different times, cold storage and treatment with IBA

    Directory of Open Access Journals (Sweden)

    Marcelo Angelo Campagnolo

    2012-02-01

    Full Text Available O objetivo do presente trabalho foi avaliar o enraizamento de estacas caulinares e radiculares de cultivares de amoreira-preta, coletadas em diferentes épocas, armazenadas a frio e tratadas com AIB. No primeiro experimento, estacas radiculares e caulinares da amoreira-preta 'Tupy' foram coletadas no momento da poda hibernal, realizada nas seguintes épocas: 07/06, 22/06, 08/07, 22/07, 06/08 e 20/08 de 2009. Já no segundo experimento, metade das estacas caulinares e radiculares da mesma cultivar foi armazenada a frio por 30 dias e a outra metade das estacas foi colocada diretamente para enraizar. Todas as estacas foram tratadas com diferentes concentrações de ácido indolbutírico (AIB: 1000, 2000, 3000 e 4000mg L-1 por 10s., além do controle composto somente por água. No terceiro experimento, foram coletadas estacas radiculares das cultivares 'Choctaw', 'Ébano', 'Guarani', 'Arapaho', 'Brazos', 'Cherokee', 'Comanche', 'Caingangue', 'Tupy' e 'Xavante' na poda realizada no dia 22 de junho do ano seguinte. As estacas foram armazenadas a frio por 30 dias e não foi realizado tratamento com AIB. Em ambos os experimentos, as estacas caulinares foram enterradas 2/3 de seu comprimento na posição vertical e as estacas radiculares foram totalmente imersas na posição horizontal, utilizando a vermiculita de grânulos finos como substrato, em telado com sombreamento de 50%. Após 90 dias, pode-se concluir que as estacas radiculares apresentam melhores resultados, devendo ser armazenadas a frio e não tratadas com AIB, mas há diferença do potencial propagativo entre os cultivares de amoreira-preta.The objective of the present research was to quantify the rizogenic potential of stems and root cuttings of blackberry cultivars, collected in different times, cold storage and treated with indolbutyric acid. In the first experiment, roots and stems cuttings of 'Tupy' blackberry were collected close to it hibernal prune and accomplished in the following

  2. Alba-domain proteins of Trypanosoma brucei are cytoplasmic RNA-binding proteins that interact with the translation machinery.

    Directory of Open Access Journals (Sweden)

    Jan Mani

    Full Text Available Trypanosoma brucei and related pathogens transcribe most genes as polycistronic arrays that are subsequently processed into monocistronic mRNAs. Expression is frequently regulated post-transcriptionally by cis-acting elements in the untranslated regions (UTRs. GPEET and EP procyclins are the major surface proteins of procyclic (insect midgut forms of T. brucei. Three regulatory elements common to the 3' UTRs of both mRNAs regulate mRNA turnover and translation. The glycerol-responsive element (GRE is unique to the GPEET 3' UTR and regulates its expression independently from EP. A synthetic RNA encompassing the GRE showed robust sequence-specific interactions with cytoplasmic proteins in electromobility shift assays. This, combined with column chromatography, led to the identification of 3 Alba-domain proteins. RNAi against Alba3 caused a growth phenotype and reduced the levels of Alba1 and Alba2 proteins, indicative of interactions between family members. Tandem-affinity purification and co-immunoprecipitation verified these interactions and also identified Alba4 in sub-stoichiometric amounts. Alba proteins are cytoplasmic and are recruited to starvation granules together with poly(A RNA. Concomitant depletion of all four Alba proteins by RNAi specifically reduced translation of a reporter transcript flanked by the GPEET 3' UTR. Pulldown of tagged Alba proteins confirmed interactions with poly(A binding proteins, ribosomal protein P0 and, in the case of Alba3, the cap-binding protein eIF4E4. In addition, Alba2 and Alba3 partially cosediment with polyribosomes in sucrose gradients. Alba-domain proteins seem to have exhibited great functional plasticity in the course of evolution. First identified as DNA-binding proteins in Archaea, then in association with nuclear RNase MRP/P in yeast and mammalian cells, they were recently described as components of a translationally silent complex containing stage-regulated mRNAs in Plasmodium. Our results are

  3. ALBA, organisation interaméricaine ou vénézuélienne ?

    Directory of Open Access Journals (Sweden)

    Jean-Jacques Kourliandsky

    2011-01-01

    Full Text Available L’«Alliance Bolivarienne des peuples de notre Amérique», ou ALBA, est de toutes les organisations interaméricaines celle dont l’identité est la plus originale. S’affirmant anti-impérialiste elle donne en effet à l’idéologie une place centrale qui commande la coopération entre ses membres. Mais l’ALBA au quotidien diffère-t-elle d’autres organisations d’intégration ? Le lien entre les différents pays qui la composent n’est-il pas au-delà de l’hommage unanime rendu à Bolivar bien davantage celui de pouvoir bénéficier du pétrole vénézuélien à un prix préférentiel ? L’ALBA, comme d’autres institutions latino-américaines, ne répond-elle pas à l’ambition d’un État, en l’occurrence ici le Venezuela, plus qu’à celle de construire un projet collectif pérenne? Ce lien entre le pétrole de son initiateur vénézuélien et l’ALBA, est une garantie pour le présent. Mais l’ALBA survivrait-elle à une alternance politique au Venezuela, ou à une chute des prix du baril affectant le nerf de l’organisation ?

  4. A structural basis for the difference in specificity between the two jacalin-related lectins from mulberry (Morus nigra) bark.

    Science.gov (United States)

    Rougé, Pierre; Peumans, Willy J; Barre, Annick; Van Damme, Els J M

    2003-04-25

    The activity and specificity of a galactose-specific and a mannose-specific jacalin-related lectin from the bark of the black mulberry (Morus nigra) tree has been re-investigated using different experimental approaches. Both lectins definitely behave as polyspecific lectins recognizing galactose, mannose, and glucose even though MornigaG and MornigaM interact preferentially with galactose and mannose, respectively. The exceptionally extended size of the carbohydrate-binding site of both lectins apparently accounts for their polyspecific character. Parallel studies with other mannose-specific jacalin-related lectins confirmed that their exclusive specificity towards mannose/glucose relies on a reduced size of their carbohydrate-binding site.

  5. Extracts of Morus nigra L. Leaves Standardized in Chlorogenic Acid, Rutin and Isoquercitrin: Tyrosinase Inhibition and Cytotoxicity.

    Science.gov (United States)

    de Freitas, Marcela Medeiros; Fontes, Pedro Ribeiro; Souza, Paula Monteiro; William Fagg, Christopher; Neves Silva Guerra, Eliete; de Medeiros Nóbrega, Yanna Karla; Silveira, Damaris; Fonseca-Bazzo, Yris; Simeoni, Luiz Alberto; Homem-de-Mello, Maurício; Oliveira Magalhães, Pérola

    Melanogenesis is a process responsible for melanin production, which is stored in melanocytes containing tyrosinase. Inhibition of this enzyme is a target in the cosmetics industry, since it controls undesirable skin conditions such as hyperpigmentation due to the overproduction of melanin. Species of the Morus genus are known for the beneficial uses offered in different parts of its plants, including tyrosinase inhibition. Thus, this project aimed to study the inhibitory activity of tyrosinase by extracts from Morus nigra leaves as well as the characterization of its chromatographic profile and cytotoxicity in order to become a new therapeutic option from a natural source. M. nigra leaves were collected, pulverized, equally divided into five batches and the standardized extract was obtained by passive maceration. There was no significant difference between batches for total solids content, yield and moisture content, which shows good reproducibility of the extraction process. Tyrosinase enzymatic activity was determined for each batch, providing the percentage of enzyme inhibition and IC50 values obtained by constructing dose-response curves and compared to kojic acid, a well-known tyrosinase inhibitor. High inhibition of tyrosinase activity was observed (above 90% at 15.625 μg/mL). The obtained IC50 values ranged from 5.00 μg/mL ± 0.23 to 8.49 μg/mL ± 0.59 and were compared to kojic acid (3.37 μg/mL ± 0.65). High Performance Liquid Chromatography analysis revealed the presence of chlorogenic acid, rutin and, its major compound, isoquercitrin. The chromatographic method employed was validated according to ICH guidelines and the extract was standardized using these polyphenols as markers. Cytotoxicity, assessed by MTT assay, was not observed on murine melanomas, human keratinocytes and mouse fibroblasts in tyrosinase IC50 values. This study demonstrated the potential of M. nigra leaf extract as a promising whitening agent of natural source against skin

  6. Extracts of Morus nigra L. Leaves Standardized in Chlorogenic Acid, Rutin and Isoquercitrin: Tyrosinase Inhibition and Cytotoxicity

    Science.gov (United States)

    Fontes, Pedro Ribeiro; Souza, Paula Monteiro; William Fagg, Christopher; Neves Silva Guerra, Eliete; de Medeiros Nóbrega, Yanna Karla; Silveira, Damaris; Fonseca-Bazzo, Yris; Simeoni, Luiz Alberto; Homem-de-Mello, Maurício; Oliveira Magalhães, Pérola

    2016-01-01

    Melanogenesis is a process responsible for melanin production, which is stored in melanocytes containing tyrosinase. Inhibition of this enzyme is a target in the cosmetics industry, since it controls undesirable skin conditions such as hyperpigmentation due to the overproduction of melanin. Species of the Morus genus are known for the beneficial uses offered in different parts of its plants, including tyrosinase inhibition. Thus, this project aimed to study the inhibitory activity of tyrosinase by extracts from Morus nigra leaves as well as the characterization of its chromatographic profile and cytotoxicity in order to become a new therapeutic option from a natural source. M. nigra leaves were collected, pulverized, equally divided into five batches and the standardized extract was obtained by passive maceration. There was no significant difference between batches for total solids content, yield and moisture content, which shows good reproducibility of the extraction process. Tyrosinase enzymatic activity was determined for each batch, providing the percentage of enzyme inhibition and IC50 values obtained by constructing dose-response curves and compared to kojic acid, a well-known tyrosinase inhibitor. High inhibition of tyrosinase activity was observed (above 90% at 15.625 μg/mL). The obtained IC50 values ranged from 5.00 μg/mL ± 0.23 to 8.49 μg/mL ± 0.59 and were compared to kojic acid (3.37 μg/mL ± 0.65). High Performance Liquid Chromatography analysis revealed the presence of chlorogenic acid, rutin and, its major compound, isoquercitrin. The chromatographic method employed was validated according to ICH guidelines and the extract was standardized using these polyphenols as markers. Cytotoxicity, assessed by MTT assay, was not observed on murine melanomas, human keratinocytes and mouse fibroblasts in tyrosinase IC50 values. This study demonstrated the potential of M. nigra leaf extract as a promising whitening agent of natural source against skin

  7. Directional hearing in the barn owl (Tyto alba).

    Science.gov (United States)

    Coles, R B; Guppy, A

    1988-05-01

    The acoustical properties of the external ear of the barn owl (Tyto alba) were studied by measuring sound pressure in the ear canal and outer ear cavity. Under normal conditions, pressure amplification by the external ear reaches about 20 dB between 3-9 kHz but decreases sharply above 10 kHz. The acoustic gain curve of the outer ear cavity alone is close to that of a finite-length exponential horn between 1.2-13 kHz with maximum gain reaching 20 dB between 5-9 kHz. Pressure gain by the facial ruff produces a maximum of 12 dB between 5-8 kHz and decreases rapidly above 9 kHz. The directional sensitivity of the external ear was obtained from pressure measurements in the ear canal. Directivity of the major lobe is explained, to a first approximation, by the sound diffraction properties of a circular aperture. Aperture size is based on the average radius (30 mm) of the open face of the ruff. Above 5 kHz, the external ear becomes highly directional and there is a 26 degree disparity in elevation between the acoustic axis of the left and right ear. In azimuth, directivity patterns are relocated closer to the midline as frequency increases and the acoustic axis moves at a rate of 20 degree/octave between 2-13 kHz. Movement of the axis can be explained, to a first approximation, by the acoustical diffraction properties of an obliquely truncated horn, due to the asymmetrical shape of the outer ear cavity. The directional sensitivity of the barn owl ear was studied by recording cochlear microphonic (CM) potentials from the round window membrane. Between 3-9 kHz, CM directivity patterns are clearly different to the directivity patterns of the external ear; CM directionality is abruptly lost above 10 kHz. Above 5 kHz, CM directivity patterns are characterized by an elongated major lobe containing the CM axis, forming a tilted band of high amplitude but low directionality (CM axial plane), closely bordered by minima or nulls. The highest directionality is found in the CM

  8. 桑树DNA连接酶Ⅰ基因MuLigⅠ的获取和序列分析%Acquisition and Sequence Analysis of MuLig Ⅰ Gene from Mulberry (Morus alba L.)

    Institute of Scientific and Technical Information of China (English)

    王桃妮; 孔卫青; 杨金宏

    2015-01-01

    为进一步研究DNA连接酶Ⅰ在类病毒复制中的作用,以感染桑花叶萎缩类病毒的桑树叶片为材料,通过在同源基因保守区设计简并引物进行PCR扩增和cDNA末端扩增,获取桑树DNA连接酶Ⅰ基因.结果获得桑树DNA连接酶Ⅰ基因MuLigI长2730bp的mRNA序列,基因编码区长2367 bp,编码788个氨基酸,含典型的DBD结构域(173~351 aa)和CD催化结构域(405~771aa),N端含有核定位(NLS)和线粒体定位信号(MLS).研究所得桑树DNA连接酶Ⅰ基因具有保守的结构域和活性位点,可能和番茄的DNA连接酶Ⅰ基因一样,参与了桑花叶萎缩类病毒的复制过程.%For further study on the role of DNA ligase Ⅰ in the replication of viroid,the DNA ligase Ⅰ gene was obtained from MMDVd-infected mulberry leaf.The gene was amplified by degenerate primer designed in the conserved domain according to the homologous genes and RACE methods.The results showed that the mRNA sequence of MuLig Ⅰ was 2730 bp in length.The open reading frame (ORF) was 2367 bp and encoding 788 amino acid residues.There were conserved DBD domain (173-351 aa),catalytic domain (405-771 aa),and mitochondrion and nuclear location signal (MLS and NLS) in MuLig Ⅰ.The MuLig Ⅰ gene in this study contained conserved structural domain and active site,and like the DNA ligase Ⅰ gene of tomato,might be involved in the replication of MMDVd.

  9. Efecto de la fertilización orgánica, la variedad y la época en el perfil polifenólico de Morus alba (L.

    Directory of Open Access Journals (Sweden)

    D. E. García

    2005-01-01

    Full Text Available Con el objetivo de detectar los principales grupos de fenoles en la biomasa de la morera y de-terminar el efecto del abonado (0, 100, 300 y 500 kg N/ha/año, la variedad (Cubana, Indonesia, Tigreada y Acorazonada y la época (periodo lluvioso y poco lluvioso, en el perfil polifenólico, se llevó a cabo una investigación con un diseño de bloques (al azar con arreglo factorial 4 x 4 x 2 y cinco repeticiones. Mediante el tamizaje fitoquímico se evaluó la presencia de siete grupos de compuestos, para posteriormente, determinar sus concentraciones. Únicamente se detectaron fenoles (FT, flavonoides (Flav y cumarinas (Cum, con presencia leve, notable o cuantiosa. Sólo se encontraron interacciones significativas entre los factores fertilización y variedad para los FT y las Cum (P<0,05. Los niveles máximos de FT y Cum encontrados en las hojas fueron de 2,86% MS (Tigreada abonada con 100 kg N/ha/año y 0,65% MS (Cubana e Indonesia fertilizadas con 100 kg N/ha/año, respectivamente. Las mayores concentraciones de FT y Cum en los tallos tiernos se observaron en la variedad Acorazonada con 100 kg N/ha/año (1,55% MS e Indonesia, con la aplicación de 500 kg N/ha/año (0,60% MS. En las hojas de las variedades (los contenidos de FT y Cum tendieron a disminuir con el incremento de los niveles de fertilización, a excepción de las concentraciones de FT, en la variedad Cubana. En el caso de los Flav, no se observó un efecto significativo de los factores fertilización, variedad y época. Solamente en los FT de las hojas, se observó un efecto representativo de la época a favor del PLL. Los resultados permiten concluir que las concentraciones de los FT se ven afectadas por los niveles de fertilización, la variedad y la época. Los contenidos de Cum presentan variaciones significativas entre los niveles de abonado orgánico y las variedades. Los niveles de Flav no muestran fluctuaciones cuantiosas con ninguna de las variables estudiadas.

  10. Determinación cualitativa de factores antinutricionales en las especies, Erithryna Poeppigiana (Walp. O. F, Trichanthera Gigantea (H y B, Morus Alba Lin, en la época de lluvia y seca.

    Directory of Open Access Journals (Sweden)

    Yandy Rodríguez Ledesma

    2014-09-01

    Full Text Available El trabajo se realizó en La Finca “La Perla”, en la localidad de Topes de Collantes, municipio de Trinidad, provincia Sancti Spíritus. Se investigó la presencia de algunos grupos de metabolitos secundarios (fenoles, taninos condensados, saponinas, cianógenos y alcaloides en la biomasa comestible de tres especies arbóreas Las muestras se pesaron y se secaron al sol. El extracto se obtuvo a partir de 5 kg de muestra con 50 ml de etanol al 95 %. Para la descripción cualitativa de los resultados se observó visualmente en la muestra la opalescencia (+, Turbidez definida (++ y precipitado (+++ y, según los resultados observados se utilizaron los siguientes criterios: presencia leve +, Moderada ++ y, abundante +++. El 100% de las especies analizadas, mostraron la presencia de factores antinutricionales; los taninos, tripterpenos y saponinas fueron los metabolitos de mayor presencia en las especies estudiadas. En la época de lluvia los taninos, alcaloides, tripterpenos, antocianinas y cumarinas se encontraron en niveles bajos. En la época de sequía no se encontraron flavonoides y las antocianinas y cumarinas estuvieron en niveles bajos.

  11. Degradation kinetics of cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside during hot air and vacuum drying in mulberry (Morus alba L.) fruit: A comparative study based on solid food system.

    Science.gov (United States)

    Zhou, Mo; Chen, Qinqin; Bi, Jinfeng; Wang, Yixiu; Wu, Xinye

    2017-08-15

    The aim of this study is to ascertain the degradation kinetic of anthocyanin in dehydration process of solid food system. Mulberry fruit was treated by hot air and vacuum drying at 60 and 75°C. The contents of cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside were determined by using high performance liquid chromatography. Kinetic and thermodynamic parameters were calculated for analysing the degradation characteristics. Model fitting results showed monomeric anthocyanin degradations were followed the second-order kinetic. Vacuum drying presented high kinetic rate constants and low t1/2 values. Thermodynamic parameters including the activation energy, enthalpy change and entropy change appeared significant differences between hot air and vacuum drying. Both heating techniques showed similar effects on polyphenol oxidase activities. These results indicate the anthocyanin degradation kinetic in solid food system is different from that in liquid and the oxygen can be regarded as a catalyst to accelerate the degradation. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Effects of Supplementation of Mulberry (Morus alba) Foliage and Urea-rice Bran as Fermentable Energy and Protein Sources in Sheep Fed Urea-treated Rice Straw Based Diet

    National Research Council Canada - National Science Library

    Yulistiani, Dwi; Jelan, Z A; Liang, J B; Yaakub, H; Abdullah, N

    2015-01-01

    A digestibility study was conducted to evaluate the effects of supplementing mulberry foliage and urea rice-bran as a source of fermentable energy and protein to 12 sheep fed diets based on urea-treated rice straw (TRS...

  13. The summary of γ-aminobutyric acid(GABA) in mulberry leaf(Morus alba L.)%桑叶中γ-氨基丁酸的研究概述

    Institute of Scientific and Technical Information of China (English)

    陈恒文; 林健荣

    2008-01-01

    γ-氨基丁酸(GABA)在动植物体内广泛存在,是中枢神经系统内重要的抑制性神经递质.本文对植物中GABA的分布、代谢、生理功能及桑叶中的GABA研究进展作一归纳与论述,以供读者更方便对GABA的了解,为研究和开发富含GABA的食品和饮料提供帮助.

  14. Study on the extraction and purification of DNJ from Morus alba L%1-脱氧野尻霉素提取精制工艺的研究

    Institute of Scientific and Technical Information of China (English)

    刘韦鋆; 赵骏; 高秀梅; 刘美平; 曾森

    2009-01-01

    目的:研究桑叶中1-脱氧野尻霉素(DNJ)的提取与精制工艺,建立1-脱氧野尻霉素(DNJ)的定性鉴别及含量测定方法.方法:本研究取桑叶药材,加70%乙醇提取,将提取液浓缩,通过大孔吸附树脂,取通过大孔吸附树脂的流出液,浓缩,通过阳离子交换树脂,用蒸馏水洗,用0.5mol/L氨水洗脱,收集洗脱液,浓缩,浓缩液通过硅胶纯化,将有杂质的组分弃去,合并含1-脱氧野尻霉素较纯的组分,浓缩,真空干燥,即得1-脱氧野尻霉素(DNJ).1-脱氧野尻霉素的定性鉴别采用薄层鉴别法,吸取溶液点于薄层板上,展开剂为乙酸乙脂-无水乙醇(2:8),碘熏法进行显色,置紫外光灯(254nm)下检视.供试品色谱中,在与对照品色谱相应的位置上,显相同颜色的荧光斑点.1-脱氧野尻霉素的含量测定采用柱前衍生化高效液相色谱法,以芴甲氧羰酰氯(Fmoc-Cl)作为衍生化试剂,在pH=8.5硼酸盐缓冲液中进行衍生化反应后,生成具有紫外吸收的DNJ-FMOC络合物,用反相高效液相色谱--紫外检测法分离测定DNJ-FMOC.采用十八烷基硅烷键合硅胶色谱柱;流动相为乙腈-0.1%冰醋酸(11:16);检测波长为254nm.结果:经测定1-脱氧野尻霉素的含量为23.2%,收率为0.355%.结论:本方法工艺流程简单、快速、收率及含量高,适合于工业生产.

  15. Micropropagação da amoreira-preta (Rubus spp. e efeito de substratos na aclimatização de plântulas = Blackberry micropropagation (Rubus spp. and effects of substrates in plants acclimatization

    Directory of Open Access Journals (Sweden)

    Fabíola Villa

    2006-01-01

    Full Text Available O objetivo do presente estudo foi aprimorar técnicas de propagação in vitro e determinar um melhor substrato para a amoreira-preta. O primeiro experimento consistiu de gemas axilares com cerca de 2 cm, oriundas de plântulas preestabelecidas in vitro da cv. Ébano, excisadas e inoculadas em meio MS, suplementado com 5 concentrações de carvão ativado e 5 de BAP. Ambos os experimentos foram inteiramente casualisados, utilizando-se in vitro 3 explantes por repetição e 4 repetições por tratamento e em casa-de-vegetação um fatorial 4x4. O pH do meio foi ajustado para 5,8 antes da adição de 6 g L-1 de ágar e da autoclavagem a 121ºC e 1 atm por 20 minutos. Após 70 dias, foram avaliados diversos parâmetros nos explantes. Maior número de folhas e maior número de raízes foram obtidos com 0,5 mg L-1 de BAP. Na presença de 3 g L-1 de carvão ativado, o número e comprimento de raízes foi maior. Maior biomassa fresca foi obtido na ausência de carvão ativado. O segundo experimento consistiu de plântulas da cv. Cherokee mantidas em condições in vitro e transplantadas para bandejas plásticas, contendo os substratos e mantidas em casa-de-vegetação. Foram testados 4 tipos de substratos. Após 100 dias de aclimatização, foram avaliados números de folhas, comprimento das raízes e da parte aérea, peso fresco e seco das raízes e peso fresco e seco da parte aérea. Foi possível concluir que a aclimatização pode ser realizada com sucesso, utilizando-se Plantmax. seguido da mistura de Plantmax. + vermiculita + casca de arroz carbonizada e vermiculita. Houve 92% de sobrevivência de plantas em todos os substratos.The objective of the present study was to achieve the improved techniques in in vitro propagation and to determine a better substrate for the blackberry. The first experiment consisted of nodal segments of plants with ±2 cm of length obtained from in vitro culture cv. Ebano, excised and inoculated in MS culture medium

  16. Enhancement of Shelf Life of Button Mushroom, Agaricus bisporus (Higher Basidiomycetes) by Fumigant Application of Lippia alba Essential Oil.

    Science.gov (United States)

    Vishwakarma, Pratima; Pandey, Abhay K; Mishra, Priyanka; Singh, Pooja; Tripathi, N N

    2015-01-01

    Eleven essential oils isolated from higher plant species were assessed against the four isolates of Verticillium fungicola found on fruiting bodies of Agaricus bisporus. Eucalyptus citriodora and Lippia alba oils were more efficacious and completely inhibited the mycelial growth of fungal isolates. L. alba oil was fungistatic and fungicidal at 10- and 20-µL concentrations against all of the isolates, respectively, and was more potent than E. citriodora oil as well as some prevalent synthetic fungicides such as benomyl, ethylene dibromide, and phosphine. Eighty microliters of L. alba oil protected 500 g of fruiting bodies of A. bisporus for up to 7 d from infection of the fungus under in vivo conditions. The findings strengthen the possibility of L. alba oil as a plant-based protectant to enhance the shelf life of A. bisporus fruiting bodies.

  17. Inhibitory effect of linalool-rich essential oil from Lippia alba on the peptidase and keratinase activities of dermatophytes.

    Science.gov (United States)

    Costa, Danielle Cristina Machado; Vermelho, Alane Beatriz; Almeida, Catia Amancio; de Souza Dias, Edilma Paraguai; Cedrola, Sabrina Martins Lage; Arrigoni-Blank, Maria de Fátima; Blank, Arie Fitzgerald; Alviano, Celuta Sales; Alviano, Daniela Sales

    2014-02-01

    Abstract Lippia alba (Miller) N.E. Brown is an aromatic plant known locally as "Erva-cidreira-do-campo" that has great importance in Brazilian folk medicine. The aim of our study was to evaluate the antidermatophytic potential of linalool-rich essential oil (EO) from L. alba and analyze the ability of this EO to inhibit peptidase and keratinase activities, which are important virulence factors in dermatophytes. The minimum inhibitory concentrations (MICs) of L. alba EO were 39, 156 and 312 µg/mL against Trichophyton rubrum, Epidermophyton floccosum and Microsporum gypseum, respectively. To evaluate the influence of L. alba EO on the proteolytic and keratinolytic activities of these dermatophytes, specific inhibitory assays were performed. The results indicated that linalool-rich EO from L. alba inhibited the activity of proteases and keratinases secreted from dermatophytes, and this inhibition could be a possible mechanism of action against dermatophytes. Due to the effective antidermatophytic activity of L. alba EO, further experiments should be performed to explore the potential of this linalool-rich EO as an alternative antifungal therapy.

  18. HYSTOLOGICAL-FUNCTIONAL SPECIFITY OF NYMPHAEA ALBA L.VEGETATIVE ORGANS

    Directory of Open Access Journals (Sweden)

    Sidorova V. N.

    2012-11-01

    Full Text Available Nymphaea alba L. belongs to aerohydrophytes and has all typical features of such ecological group. We found out the followings anatomic and functional features which are adaptation to the surplus of water: 1 formation of astrosklereid, which are the mechanical fabrics; 2 presence of large intercells which serve as plant fixation; 3 absence of stomas on the lower side of leaf and submarine organs that alterate the interchange of gases. The mycrochemical ash analysis of plant vegetative organs showed the presence of crystals of strontium, sulfur, potassium, ferrum, calcium, sodium, nitrogen, which vary by accumulation, form, and sizes, in vegetative organs (leaf, root and stem. We proved that quantitative, anatomical, and physiological peculiarities of Nymphaea alba L. vegetative organs uncover the mechanism of adaptation of aerohydrophytes to environment factors. The adaptative mechanisms of plant and their functioning are changed under influence of surplus of water.

  19. Avian pox virus infection in a common barn owl (Tyto alba in southern Brazil

    Directory of Open Access Journals (Sweden)

    Gilberto D. Vargas

    2011-07-01

    Full Text Available A young common barn owl (Tyto alba was referred to the Núcleo de Reabilitação da Fauna Silvestre (Nurfs, Federal University of Pelotas (UFPel, after been found in a barn of a brick factory in the urban area of Pelotas, Rio Grande do Sul, Brazil. The bird was apathic, weak and with crusty lesions in the featherless areas (eyes, beak, legs, and died soon after arrival at Nurfs. Necropsy and histopathological examination of the lesions were carried out. The hyperplasia and hypertrophy of the cutaneous lesions, several eosinophilic intracyto-plasmic inclusion bodies in epithelial cells (Bollinger bodies, as well as particles characteristic of poxvirus, observed by electronic microscopy, confirmed the infection by avian poxvirus, what highlights the importance of Tyto alba as carrier of the virus in the wild.

  20. MAJOR SUMMER-INDUCED THERMAL RISKS IN THE ALBA IULIA – TURDA DEPRESSION

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    CĂTĂLINA MĂRCULEȚ

    2014-05-01

    Full Text Available Major summer-induced thermal risks in the Alba Iulia - Turda Depression. The study deals with warm-season phenomena, featuring positive, sometimes extremely high temperatures that may have a negative influence both on people’s health and economic activities. This category includes tropical waves of heat, absolute maximum temperatures, maximum frequency registered outside the specific interval of summer thermal regime: summer days, as well as tropical days and nights. The paper describes the conditions in which these phenomena occur, average and maximum occurrence rate and evolution trends likely to impair the economy at large, and especially the wide variety of crops covering large surfaceareas in the Alba Iulia - Turda Depression enjoying a Föehn regime.

  1. The bioindicative potential evaluation of Tabebuia alba (Cham. Sandwith, Bignoniaceae, in urban atmospheric pollution

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    César Vinícius Carvalheiro

    2013-08-01

    Full Text Available This study aimed to evaluate the existence of leaf anatomic characteristics in Tabebuia alba changed by air pollutants, which could be used as tool for a bioindication program. The quantification of mutagenic events on pollen grains also were measured. For this, median leaves and pre-anthesis flowers were collected from the adult plants from three places of Curitiba and one place in Araucaria, all nearby to the air monitoring stations. The comparison of the four study sites showed a reduction in leaf area, an increasing of stomatal density, subepidermic layer, epidermis in both faces and the amount of micronucleus. Also, there was reduction of chlorophyllian parenchymas at the site where there was the higher average for the ozone level. It was concluded that these modifications might be a consequence of the effect of troposferic pollution on T. alba plants. However, further studies with this species would be necessary to confirm its potential for bioindication.

  2. Pharmacognostical Standardization of Upodika- Basella alba L.: An Important Ayurvedic Antidiabetic Plant

    Science.gov (United States)

    Shantha, T R; Patchaimal, P; Reddy, M Prathapa; Kumar, R Kishore; Tewari, Devesh; Bharti, Vandana; Venkateshwarlu, G; Mangal, A K; Padhi, M M; Dhiman, K S

    2016-01-01

    Objective: To establish the pharmacognostic standards for the correct identification and standardization of an important Antidiabetic plant described in Ayurveda. Materials and Methods: Standardization was carried out on the leaf and stem of Basella alba L. with the help of the macro-morphological, microscopic, physicochemical and qualitative phytochemical studies. Results: Several specific characters were identified viz. clustered calcium oxalate crystals in the cortex region, absence of trichomes, succulent, thick, mucilaginous, fibrous stem. Rubiaceous type of stomata on both sides of the leaf. Quantitative microscopy along with physicochemical and qualitative phytochemical analysis were also established. Conclusion: The pharmacognostic standards could serve as the reference for the proper identification of the Basella alba L. which is an important anti-diabetic plant described in Ayurveda.

  3. Antibacterial activity of Phyllantus emblica, Coriandrum sativum, Culinaris medic, Lawsonia alba and Cucumis sativus.

    Science.gov (United States)

    Khan, Dawood Ali; Hassan, Fouzia; Ullah, Hanif; Karim, Sabiha; Baseer, Abdul; Abid, Mobasher Ali; Ubaidi, Muhammad; Khan, Shujaat Ali; Murtaza, Ghulam

    2013-01-01

    Present study deals with the demonstration of the antibacterial activity of very common medicinal plants of Pakistani origin i.e., Phyllantus emblica, Coriandrum sativum, Culinaris medic, Lawsonia alba and Cucumis sativus. The extracts were prepared in crude form by the use of hydro-alcoholic solution and were screened for antibacterial activity against various bacterial species by disk diffusion method. Assay was performed using clinical isolates of B. cereus, S. aureus, P. aeruginosa and E. coli. Crude extract of Phyllantus emblica fruit exhibited strong activity against standard cultures of all studied bacteria. Lawsonia alba showed good activity against standard cultures of all the used microorganisms. Coriandrum sativum was effective only against Bacillus cereus, while Cucumis sativus and Culinaris medic showed poor activity against Pseudomonas aeruginosa only. Hence, Phyllantus emblica exhibited strong antibacterial activity against a wide range of bacteria it means that Phyllantus emblica extract contains some compounds which have broad spectrum of bactericidal activity.

  4. Therapeutic activity of crude ethanolic extract of Artemisia herba alba against Trypanosoma evansi in rabbits

    Science.gov (United States)

    Awad, Fathy M.; Hasan, Zainal Abidin Abu; Osman, Abdinasir Yusuf; Ibrahim, Nazlina

    2013-11-01

    The present work was conducted to evaluate the antitrypanosomal efficacy of crude ethanolic extract (CEE) of the aerial parts of Artemisia herba alba against Trypanosoma evansi infection in an animal model. The results indicated low levels of parasitaemia in rabbits administered with crude ethanolic extract (CEE) compared to those from the negative control group. Similarly, there was also haematologically significant difference (p<0.05) where low mean levels of packed cell volume (PCV) was observed in Groups 1-4 respectively. In contrast, there was no statistically significant difference in almost all investigated parameters between positive control and treatment groups of animals. In conclusion, both CEE of A. herba-alba and Berenil® showed relatively a parasitaemia and normal haematological values in infected rabbits, thereby confirming their antiparasitic properties.

  5. El ALBA y los “anti”. Un proyecto de integración alternativo latinoamericano

    OpenAIRE

    Adolfo A. Abadía

    2014-01-01

    Reseña del libro:Bagley, B. M. y Defort M. (Eds.) (2014). ¿La hegemonía norteamericana en declive? El desafío del ALBA y la nueva integración latinoamericana del siglo XXI. ColecciónEl sur es cielo roto. Cali, Colombia: Facultad de Derecho y Ciencias Sociales,Universidad Icesi. 640 pp.

  6. El ALBA y los “anti”. Un proyecto de integración alternativo latinoamericano

    Directory of Open Access Journals (Sweden)

    Adolfo A. Abadía

    2014-12-01

    Full Text Available Reseña del libro:Bagley, B. M. y Defort M. (Eds. (2014. ¿La hegemonía norteamericana en declive? El desafío del ALBA y la nueva integración latinoamericana del siglo XXI. ColecciónEl sur es cielo roto. Cali, Colombia: Facultad de Derecho y Ciencias Sociales,Universidad Icesi. 640 pp.

  7. Fluvial valleys on Alba Patera, Mars, viewed by HRSC/MEx camera

    Science.gov (United States)

    Ansan, V.; Mangold, N.; Masson, P. L.; Neukum, G.

    2009-12-01

    Alba Patera is the northernmost shield volcano of the Tharsis bulge, on which valley networks have been identified in Viking images. Valleys are mainly distributed on the northern side of volcano, with a parallel to dendritic pattern associated with a very high drainage density of 2.3 km-1, comparable to those observed on Hawaiian volcanoes (Gulick and Baker, Nature 341, 1989; and JGR 95, 1990). They are older than sets of normal faults cutting Alba Patera, and dated of the Amazonian Period, but the age of the volcano itself (Late Hesperian-Early Amazonian) implies an age for valleys younger than that of classical valley networks formed during early Mars. These valley networks have been revisited by the HRSC stereo camera enable to generate Digital Elevation Models (DEM) with a spatial gridding of History, their original morphology is partially smoothened by a dust mantle in high resolution images, but this mantling does not seem to have filled these valleys significantly. Images also confirm that valleys located to the north are likely the result of hydrologic erosion in volcanic ash as proposed previously by Gulick and Baker (1990). Previously unrecognized valley networks have been observed on the eastern and southeastern sides of Alba Patera, where volcanic flows are well developed and less blanketed by dust or ash deposits. They are shallower than northern ones, and some prints of seepage at the front of lava flows have been identified indicating that liquid water percolation was an active process in this lithology. In summary, 3D characteristics of valleys on Alba Patera do not suggest a sustained fluvial activity unlike what could be derived by their 2D properties such as the high drainage density. Episodic snowmelt following snow deposition, or eventually episodic rainfall, could be at the origin of these shallow valleys.

  8. Isoprene and terpenoid emissions from Abies alba: Identification and emission rates under ambient conditions

    Science.gov (United States)

    Pokorska, Olga; Dewulf, Jo; Amelynck, Crist; Schoon, Niels; Šimpraga, Maja; Steppe, Kathy; Van Langenhove, Herman

    2012-11-01

    In this study, biogenic volatile organic compound (BVOC) emissions from Abies alba were studied under ambient conditions in Flanders (Belgium). Emission patterns and rates were investigated from April till November 2010 by using the dynamic branch enclosure technique. The present work revealed that A. alba is an isoprene emitter, with isoprene accounting for 86-93% of total BVOC emissions, except during budburst (67%) in May. The emission spectrum of A. alba consisted of 27 compounds. Next to isoprene, the main emitted compounds were α-pinene, β-pinene, camphene and limonene. BVOC emissions showed a peak in June after development of the young needles, followed by a constant emission during summer months and September and a decrease in October. In all the samples isoprene was the most abundant compound with standardized emission rates between 27 μg g(dw)-1 h-1 in June and 4.6 μg g(dw)-1 h-1 in October, while the total standardized terpenoid emission rates ranged from 2.85 μg g(dw)-1 h-1 in June to 0.26 μg g(dw)-1 h-1 in October. The obtained average β coefficients according to the temperature dependent algorithm of Guenther et al. (1993) during April-June, July, August and September-October were as follows: for terpenoids 0.12 ± 0.03, 0.11 ± 0.05, 0.12 ± 0.04, 0.24 ± 0.01 K-1 and sesquiterpenes (SQTs) 0.09 ± 0.02, 0.11 ± 0.01, 0.10 ± 0.05, 0 K-1, respectively. Overall, isoprene detected in this study was never quantified in previous studies on A. alba and this finding could have a significant impact on the regional BVOCs budget. Therefore, the result of this study is very important for modeling and local air quality.

  9. Female plumage spottiness signals parasite resistance in the barn owl (Tyto alba)

    OpenAIRE

    Roulin, A.; Riols, C; Dijkstra, C; Ducrest, A. L.

    2001-01-01

    The hypothesis that extravagant ornaments signal parasite resistance has received support in several species for ornamented males but more rarely for ornamented females. However, recent theories have proposed that females should often be under sexual selection, and therefore females may signal the heritable capacity to resist parasites. We investigated this hypothesis in the socially monogamous barn owl, Tyto alba, in which females exhibit on average more and larger black spots on the plumage...

  10. Mycocoenology in Abies alba Miller woods of central-southern Tuscany (Italy)

    OpenAIRE

    Angela Laganà; Elena Salerni; Carla Barluzzi; Claudia Perini; Vincenzo de Dominicis

    2014-01-01

    Numerous reports indicate that fir woods in central and northern Europe have recently been damaged by increasing pollution. It has been demonstrated that fungi can be good bioindicators of forest health status. In polluted areas the production of fruit bodies generally declines and the fungal biodiversity, especially of symbiotic species, is reduced. Here we report the results of a survey of the fungal and plant communities in woods of Abies alba Miller in central-southern Tuscany, already st...

  11. Insights into some physiological and biochemical responses of Populus alba and Populus nigra to lead contamination

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    Elham Etemadi

    2016-12-01

    Full Text Available The effects of lead (PbCl2 increment, under hydroponic conditions up to 15, 45 and 90 mg/l in presence of EDTA, on some physiological and biochemical traits of one year old saplings of P. nigra and P. alba, were investigated. Six weeks after establishing in target concentration, the amount of lead, biomass, water, soluble sugars, proline, electrolyte leakage, malondialdehyde, and pigments were assessed in different organs. The results revealed that with increasing lead concentration in culture medium in the studied period, the amount of lead in saplings increased, but no effect was observed on their biomass. In both species the magnitude of lead accumulation in root was higher than leaf. P. nigra had more water and less soluble sugars than P. alba. The concentration of soluble sugars increased up to 1.5 times with lead increment in both species, but proline content increased only in P. nigra up to 2 times and remained constant in P. alba. Elevation of electrolyte leakage in saplings of P. nigra in excess lead treatment was accompanied by no change in malondialdehyde content. Concentrations of pigments were not affected by lead, and only the ratio of chlorophyll a to b in P. nigra increased in high lead concentration. In general both species accumulated high extent of lead in their organs. But it seems that P. nigra, at least with respect of enhancing plasma membrane permeability, increasing proline and the ratio of chlorophyll a to b, was more sensitive to this toxic metal in compare with P. alba.

  12. Composition, anti-quorum sensing and antimicrobial activity of essential oils from Lippia alba

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    Jesus Olivero-Verbel

    2014-09-01

    Full Text Available Many Gram-negative pathogens have the ability to produce N-acylhomoserine lactones (AHLs as signal molecules for quorum sensing (QS. This cell-cell communication system allows them to coordinate gene expression and regulate virulence. Strategies to inhibit QS are promising for the control of infectious diseases or antibiotic resistant bacterial pathogens. The aim of the present study was to evaluate the anti-quorum sensing (anti-QS and antibacterial potential of five essential oils isolated from Lippia alba on the Tn-5 mutant of Chromobacterium violaceum CV026, and on the growth of the gram-positive bacteria S. aureus ATCC 25923. The anti-QS activity was detected through the inhibition of the QS-controlled violacein pigment production by the sensor bacteria. Results showed that two essential oils from L. alba, one containing the greatest geranial:neral and the other the highest limonene:carvone concentrations, were the most effective QS inhibitors. Both oils also had small effects on cell growth. Moreover, the geranial/neral chemotype oil also produced the maximum zone of growth inhibition against S. aureus ATCC 25923. These data suggest essential oils from L. alba have promising properties as QS modulators, and present antibacterial activity on S. aureus.

  13. An airborne actinobacteria Nocardiopsis alba isolated from bioaerosol of a mushroom compost facility.

    Science.gov (United States)

    Paściak, Mariola; Pawlik, Krzysztof; Gamian, Andrzej; Szponar, Bogumiła; Skóra, Justyna; Gutarowska, Beata

    2014-01-01

    Actinobacteria are widely distributed in many environments and represent the most important trigger to the occupant respiratory health. Health complaints, including hypersensitivity pneumonitis of the workers, were recorded in a mushroom compost facility (MCF). The studies on the airborne bacteria were carried out to find a possible microbiological source of these symptoms. Culture analysis of compost bioaerosols collected in different location of the MCF was performed. An assessment of the indoor microbial exposure revealed bacterial flora of bioaerosol in the mushroom compost facility represented by Bacillus, Geobacillus, Micrococcus, Pseudomonas, Staphylococcus spp., and actinobacterial strain with white aerial mycelium. The thermotolerant actinobacterial strain of the same morphology was repeatedly isolated from many locations in MCF: air, compost sample, and solid surface in production hall. On the base of complex morphological, chemotaxonomic, and phylogenetic characteristics, the isolate has been classified as Nocardiopsis alba. Dominant position of N. alba in microbial environment of the mushroom compost facility may represent an indicator microorganism in compost bioaerosol. The bioavailability of N. alba in mushroom compost facility creates potential risk for the health of workers, and the protection of respiratory tract and/or skin is strongly recommended.

  14. Analysis of synonymous codon usage in chloroplast genome of Populus alba

    Institute of Scientific and Technical Information of China (English)

    ZHOU Meng; LONG Wei; LI Xia

    2008-01-01

    The pattern of codon usage in the chloroplast genome of Populus alba was investigated.Correspondence analysis (a commonly used multivariate statistical approach) and method of effective number of codons (ENc)-plot were conducted to analyze synonymous codon usage.The results of correspondence analysis showed that the distribution of genes on the major axis was significantly correlated with the frequency of use of G+C in synonymously variable third position of sense codon (GC3S),(r=0.349),and the positions of genes on the axis 2 and axis 3 were significantly correlated with CAI (r=-0.348,p<0.01 and r=0.602,p<0.01).The ENc for most genes was similar to that for the expected ENc based on the GC3S,but several genes with low ENC values were lying below the expected curve.All of these data indicated that codon usage was dominated by a mutational bias in chloroplast genome of P.alba.The selection in nature for translational efficiency only played a minor role in shaping codon usage in the chloroplast genome of P.alba.

  15. Release and dispersal of basidiospores from Amanita muscaria var. alba and their infiltration into a residence.

    Science.gov (United States)

    Li, De-Wei

    2005-11-01

    Release and dispersal of basidiospores of Amanita muscaria var. alba and their potential to infiltrate a nearby residence were investigated. Basidiospore release mainly occurred in the first three days following the expansion of the caps. The concentrations of released basidiospores near basidiomata were 77 137, 75 062, and 41 738 spores m(-3) in the first three days, respectively, with the highest concentration at 281 738 spores m(-3) air. After three days, the concentration dropped by 95%. At the second location, airborne basidiospore concentrations dropped 96-99% after three days with the concentrations of 940, 575, and 1359 spores m(-3) in the first three days, respectively. The diurnal pattern showed a relatively extended night peak. Relative humidity and dew were positively correlated with basidiospore release and short distance dispersal. Rain and rain rate were positively correlated with basidiospore release, but not correlated with short distance dispersal. The basidiospore release period of Amanita muscaria var. alba was short, but within such a period it released a large amount of basidiospores. However, only less than 5% of basidiospores released were dispersed to the second location 5.2 m away and 2.7 m above the basidiomata. Only Amanita muscaria var. alba showed a low potential of infiltrating the residence.

  16. Some coccidial parasites of the lizard Amphisbaena alba (Reptilia: Amphisbaenia: Amphisbaenidae

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    Ralph Lainson

    2003-10-01

    Full Text Available Five parasites are described in the lizard Amphisbaena alba (Amphisbaenidae from the state of Pará, North Brazil. Mature oocysts of Choleoeimeria amphisbaenae n. sp., are passed already mature in the faeces. They are ellipsoidal-cylindrical, average 33.7 x 22.8 µm and are devoid of micropyle, oocyst residuum or polar body. The colourless wall is smooth and of 2 layers. The 4 dizoic sporocysts have no Stieda body and average 13 x 9.3 µm. Endogenous stages develop in the epithelial cells of the gall-bladder in the manner described for the genus and may cause extensive tissue damage. Sporulation of Isospora capanemaensis n. sp., is completed 3 days after the oocysts are voided in the faeces. They average 14.8 x 14.5 µm and have no micropyle, oocyst residuum or polar body. The 2 tetrazoic sporocysts are pear-shaped, average 8.6 x 6.6 and have an inconspicuous Stieda body. Endogenous development is in the epithelial cells of the ileum, and heavy infections cause considerable tissue destruction. Multisporocystic oocysts passed in the faeces of one A. alba possibly originated from an invertebrate host ingested by the lizard. A globidium-like cyst in the digestive tract of A. alba measured 105 x 85 µm and contained many hundreds of merozoites. A stained kidney smear of the same lizard revealed the presence of an unidentified parasite producing multinucleate cyst-like stages.

  17. La aljama de los judíos de Alba de Tormes en la Edad Media

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    María Fuencisla García Casar

    2002-01-01

    Full Text Available Alba de Tormes fue una de las veinticuatro localidades salmantinas que contó con población judía a lo largo de los siglos medievales. Destacó la pujante aljama por su activa participación en los asuntos del concejo, por la estima que alcanzaron sus médicos, por la confianza de la que hizo gala el Estudio salmantino al confiar a judíos albenses la recaudación de sus rentas y, muy especialmente destacó, por ser con toda probabilidad el origen de la familia Zacut, cuyo miembro más preclaro fue el astrónomo y matemático Abrahán Zacut, cuyos cálculos astronómicos manejó su contemporáneo Cristóbal Colón.Alba de Tormes has been one of 24 localities of Salamanca which counted with Jewish population during the medieval centuries. The puissant aljama was conspicuous because of its active participation in the counsel affairs, the esteen that has been caugth up with theír Jewish doctors, the reliance that was the pride of the Studies of Salamanca, by trusting to Jewish from Alba de Tormes the collection of their rents and specially, probabiy because of being the little country of the great astronomic and mathematic Abrahán Zacut, whose astronomical calculations were managed by his contemporaneous Christopher Colombus.

  18. WINE ROAD - AN INSTRUMENT FOR THE VALORISATION OF WINE TOURISM POTENTIAL CASE STUDY: ALBA COUNTY VINEYARDS

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    UNGUREANU Mihaela

    2015-12-01

    Full Text Available The main aim of this study is to highlight the wine-growing and wine-making potential of Alba County and the way it can be valorised. Alba county has a rich winegrowing and wine-making heritage, a fact which is due to the long-standing tradition of winegrowing on these area, as well as to the characteristics of the natural factors (relief, geology, climate, soil, favourable for obtaining high-quality wines, the reputation of which has been acquired at national and international competitions. In order to render useful the wine tourism resources, the development of a specific infrastructure is needed, as well as the creation of complex tourist products, able to satisfy a wide range of tourist motivations. An efficient instrument to make productive the wine potential of a region is the „Wine Road" – a tourist trail which includes the tourist attractions of a delimited area, usually with a controlled designation of origin, and also a diverse range of tourist services (transportation, accommodation, catering leisure etc.. In Alba County, the „Wine Road" can be considered as a tourist attraction in itself, but also a means of harnessing the rich cultural-historical and natural heritage and, implicitly, the wine-growing and wine-making heritage.

  19. HPLC profiles and biomarker contents of Australian-grown Salvia miltiorrhiza f. alba roots.

    Science.gov (United States)

    Li, Chun Guang; Sheng, Shu Jun; Pang, Edwin C K; May, Brian; Xue, Charlie Chang Li

    2009-07-01

    Salvia miltiorrhiza f. alba (Baihua Danshen) is a Chinese medicinal herb commonly used for treating cardiovascular disease. It has been grown in Australia, although the quality of its main medicinal part (dried root) has not been assessed. In this study, we investigated HPLC profiles and biomarker contents of Australian-grown S. miltiorrhiza f. alba roots. Patterns of HPLC profiles were established in MeOH, and aqueous extracts in terms of number of common characteristic peaks and their relative retention times. The contents of three tanshinone biomarkers (cryptotanshinone (3), tanshinone I (1), and tanshinone IIA (2)) were significantly higher (pplants than those of two-year-old plants. In contrast, salvianolic acid B (4) content was significantly higher in the roots of two-year-old plants than in those of one-year-old plants. The findings suggest that the biomarker contents in Australian-grown S. miltiorrhiza f. alba roots vary with the growth periods of the plants, which may be important in determining the optimal harvest time for the plant roots with targeted levels of tanshinones and salvianolic acid B (4).

  20. The biology of flowering and structure of selected elements of Cornus alba L. flowers

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    Agata Konarska

    2012-12-01

    Full Text Available The biology of flowering and the micromorphology of Cornus alba flowers were studied using light and scanning electron microscopy. The flowering of white dogwood in 2008 lasted 35 days, and the lifespan of a single flower was 3 days. The number of flowers per inflorescence was variable (on the average, it was 89. The largest group of insects visiting the flowers of C. alba comprised Hymenoptera (mainly bees and andrenids, then ants, dipterans and beetles. They foraged the dogwood flowers most intensively between 11.00 and 15.00. The inconspicuous four-petalled flowers of C. alba were characterised by the occurrence of T-shaped, two-armed non-glandular trichomes covering the receptacle as well as observed on the petals of the corolla, the style of the pistil and the anthers in a smaller number. The trichomes were covered by a thick cuticle with characteristic outgrowths. They contained a living protoplast, and plastids were observed in the cytoplasm of the trichome cells. In addition, anomocytic stomata were found in the epidermis of the receptacle and in the epidermis of the corolla petals. The stigma of the pistil and the adaxial epidermis of the petals were composed of very numerous conical papillae.

  1. Insecticidal activity of powder and essential oil of Cryptocarya alba (Molina Looser against Sitophilus zeamais Motschulsky

    Directory of Open Access Journals (Sweden)

    Juan J Pinto

    2016-03-01

    Full Text Available Cereals constitute a relevant part of human and domestic animal diet. Under storage conditions, one of the most significant problems of these crops is insect pests as the maize weevil (Sitophilus zeamais Motschulsky. This insect species is usually controlled by means of synthetic insecticides but problems as toxic residues and resistance has led to the search for more friendly control alternatives such as botanical insecticides. The aim of this research was to evaluate, under laboratory conditions, the insecticidal properties of the powder and the essential oil of peumo (Cryptocarya alba [Molina] Looser; Lauraceae leaves against S. zeamais. The variables assessed were toxicity by contact and fumigant activity, adult emergence (F1, repellent effect, and impact on wheat (Triticum aestivum L. seed germination. A completely randomized design was used with five treatments and 10 replicates. The higher mortality levels were obtained at 80 g powder kg-1 grain and 40 mL essential oil kg-1 grain of C. alba; in both cases, the mortality of adult S. zeamais surpassed 80%. The emergence of adults S. zeamais (F1 was reduced by 100% at 80 g powder kg-1 grain and 40 mL essential oil kg-1 grain. Germination of wheat seeds treated with C. alba powder and essential oil was not affected. Both, the powder and the oil treatments showed repellent effect, but not fumigant activity.

  2. Composition, anti-quorum sensing and antimicrobial activity of essential oils from Lippia alba.

    Science.gov (United States)

    Olivero-Verbel, Jesus; Barreto-Maya, Ana; Bertel-Sevilla, Angela; Stashenko, Elena E

    2014-01-01

    Many Gram-negative pathogens have the ability to produce N-acylhomoserine lactones (AHLs) as signal molecules for quorum sensing (QS). This cell-cell communication system allows them to coordinate gene expression and regulate virulence. Strategies to inhibit QS are promising for the control of infectious diseases or antibiotic resistant bacterial pathogens. The aim of the present study was to evaluate the anti-quorum sensing (anti-QS) and antibacterial potential of five essential oils isolated from Lippia alba on the Tn-5 mutant of Chromobacterium violaceum CV026, and on the growth of the gram-positive bacteria S. aureus ATCC 25923. The anti-QS activity was detected through the inhibition of the QS-controlled violacein pigment production by the sensor bacteria. Results showed that two essential oils from L. alba, one containing the greatest geranial:neral and the other the highest limonene:carvone concentrations, were the most effective QS inhibitors. Both oils also had small effects on cell growth. Moreover, the geranial/neral chemotype oil also produced the maximum zone of growth inhibition against S. aureus ATCC 25923. These data suggest essential oils from L. alba have promising properties as QS modulators, and present antibacterial activity on S. aureus.

  3. Découvertes archéologiques à Alba Iulia et une conversion des Hongrois

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    Răzvan Theodorescu

    2014-12-01

    Full Text Available Superposés par l’autel de la basilique romane St Michel de Alba Iulia,les restes d’un baptistère circulaire,daté au Xe siècle, témoignent d’une technique de construction fréquente dans l’espace byzantin. Une chronique byzantine (Cedrenos-Skylitzes fait mention du baptême à Constantinople, vers le milieu du même siècle, d’un chef hongrois, Gyula «archonte des Turcs» qui retourna dans ses terres accompagné par un «évêque de la Turquie», Hiérothée. On sait que, pour les Byzantins de cette époque-là, les Magyars étaient des Turcs et que la Turquie représentait le Sud de la Hongrie et la zone transylvaine. Il est donc évident que la résidence de Gyula ne saurait être autre que «Alba de Gyula», donc, Alba Iulia, jusqu’où il était arrivée la première vague de pénétration hongroise, suivant la rivière Mureş, en quête de salines. Dès 1977, ai-je déjà avancé l’hypothèse d’une première christianisation des Hongrois, faite à Alba Iulia vers 950, sous le signe de Byzance, une bonne moitié de siècle avant que le petit-fils de Gyula, le roi Etienne I de Hongrie, n’en dirige une nouvelle, faite cette fois sous le signe de Rome. De nouvelles découvertes à Alba Iulia ont mis en évidence une église de type «croix grecque inscrite», datée toujours au Xe siècle, ce qui nous permet d’affirmer que, de par l’évêque grec, le sanctuaire cruciforme et le baptistère circulaire, on peut parler d’un horizon de vie byzantine en Transylvanie, avant l’année 1000.

  4. Asteraceae Artemisia campestris and Artemisia herba-alba Essential Oils Trigger Apoptosis and Cell Cycle Arrest in Leishmania infantum Promastigotes

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    Zohra Aloui

    2016-01-01

    Full Text Available We report the chemical composition and anti-Leishmania and antioxidant activity of Artemisia campestris L. and Artemisia herba-alba Asso. essential oils (EOs. Our results showed that these extracts exhibit different antioxidant activities according to the used assay. The radical scavenging effects determined by DPPH assay were of IC50 = 3.3 mg/mL and IC50 = 9.1 mg/mL for Artemisia campestris and Artemisia herba-alba essential oils, respectively. However, antioxidant effects of both essential oils, determined by ferric-reducing antioxidant power (FRAP assay, were in the same range (2.3 and 2.97 mg eq EDTA/g EO, resp., while the Artemisia herba-alba essential oil showed highest chelating activity of Fe2+ ions (27.48 mM Fe2+. Interestingly, we showed that both EOs possess dose-dependent activity against Leishmania infantum promastigotes with IC50 values of 68 μg/mL and 44 μg/mL for A. herba-alba and A. campestris, respectively. We reported, for the first time, that antileishmanial activity of both EOs was mediated by cell apoptosis induction and cell cycle arrest at the sub-G0/G1 phase. All our results showed that EOs from A. herba-alba and A. campestris plants are promising candidates as anti-Leishmania medicinal products.

  5. Asteraceae Artemisia campestris and Artemisia herba-alba Essential Oils Trigger Apoptosis and Cell Cycle Arrest in Leishmania infantum Promastigotes

    Science.gov (United States)

    Messaoud, Chokri; Haoues, Meriam; Neffati, Noura; Bassoumi Jamoussi, Imen; Essafi-Benkhadir, Khadija; Boussaid, Mohamed; Karoui, Habib

    2016-01-01

    We report the chemical composition and anti-Leishmania and antioxidant activity of Artemisia campestris L. and Artemisia herba-alba Asso. essential oils (EOs). Our results showed that these extracts exhibit different antioxidant activities according to the used assay. The radical scavenging effects determined by DPPH assay were of IC50 = 3.3 mg/mL and IC50 = 9.1 mg/mL for Artemisia campestris and Artemisia herba-alba essential oils, respectively. However, antioxidant effects of both essential oils, determined by ferric-reducing antioxidant power (FRAP) assay, were in the same range (2.3 and 2.97 mg eq EDTA/g EO, resp.), while the Artemisia herba-alba essential oil showed highest chelating activity of Fe2+ ions (27.48 mM Fe2+). Interestingly, we showed that both EOs possess dose-dependent activity against Leishmania infantum promastigotes with IC50 values of 68 μg/mL and 44 μg/mL for A. herba-alba and A. campestris, respectively. We reported, for the first time, that antileishmanial activity of both EOs was mediated by cell apoptosis induction and cell cycle arrest at the sub-G0/G1 phase. All our results showed that EOs from A. herba-alba and A. campestris plants are promising candidates as anti-Leishmania medicinal products. PMID:27807464

  6. Mosquito larvicidal and ovicidal properties of Eclipta alba (L.) Hassk (Asteraceae) against chikungunya vector, Aedes aegypti (Linn.) (Diptera:Culicidae)

    Institute of Scientific and Technical Information of China (English)

    M Govindarajan; P Karuppannan

    2011-01-01

    Objective: The present study deals with the investigation of larvicidal and ovicidal activities of benzene, hexane, ethyl acetate, methanol and chloroform leaf extract of Eclipta alba (E. alba) against dengue vector, Aedes aegypti (Ae. Aegypti). Methods: Twenty five early III instar larvae of Ae. aegypti was exposed to various concentrations (50-300 ppm) and was assayed in the laboratory by using the protocol of WHO 2005; the 24 h LC50 values of the E. alba leaf extract was determined by Probit analysis. For ovicidal activity, slightly modified method of Su and Mulla was performed. The ovicidal activity was determined against Ae. aegypti to various concentrations ranging from 100-350 ppm under the laboratory conditions. The egg hatch rates were assessed 48 h post treatment. Results: The LC50 values of benzene, hexane, ethyl acetate, methanol and chloroform extract of E. alba against early third instar larvae of Ae. aegypti were 151.38, 165.10, 154.88, 127.64 and 146.28 ppm, respectively. Maximum larvicidal activity was observed in the methanol extract followed by chloroform, benzene, ethyl acetate and hexane extract. No mortality was observed in control. Among five solvent tested the methanol extract was found to be most effective for ovicidal activity against Ae. aegypti. The methanol extracts exerted 100% mortality (zero hatchability) at 300 ppm. Conclusions: From the results it can be concluded the crude extract of E. alba was an excellent potential for controlling Ae. aegypti mosquito.

  7. Quimiotipos, Extracción, Composición y Aplicaciones del Aceite Esencial de Lippia alba

    Directory of Open Access Journals (Sweden)

    G.A. LINDE

    2016-03-01

    Full Text Available RESUMO Lippia alba é uma planta amplamente distribuída nas zonas tropicais, subtropicais e temperadas das Américas, África e Ásia. O óleo essencial de L. alba tem sido amplamente estudado, entretanto apresenta variações de produção. Portanto este estudo teve como objetivo realizar uma revisão dos principais quimiotipos, métodos de extração, composição e aplicação do óleo essencial de L. alba. Neste estudo são discutidos os principais quimiotipos e sua relação com fatores genéticos e características morfológicas. Também são discutidos os fatores que afetam o rendimento de produção, composição química, métodos de extração e do uso e da atividade biológica do óleo essencial de L. alba. Apesar da vasta literatura sobre os óleos essenciais de L. alba, ainda desenvolvimento de aplicações para a produção de cosméticos, fármacos e alimentos, bem como faltam definições agronomicas sobre o cultivo e melhoramento desta planta.

  8. The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen after transportation

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    Joseânia Salbego

    Full Text Available This study investigated the effects of the essential oil (EO from Lippia alba on biochemical parameters related to oxidative stress in the brain and liver of silver catfish (Rhamdia quelen after six hours of transport. Fish were transported in plastic bags and divided into three treatments groups: control, 30 µL L- 1 EO from L.alba and 40 µL L-1 EO from L.alba. Prior to transport, the fish were treated with the EO from L. alba (200 µL L -1 for three minutes, except for the control group. Fish transported in bags containing the EO did not have any alterations in acetylcholinesterase, ecto -nucleoside triphosphate diphosphohydrolase and 5'nucleotidase activity in the brain or superoxide dismutase activity in the liver. The hepatic catalase (CAT, glutathione-S-transferase (GST, glutathione peroxidase (GPx, nonprotein thiol and ascorbic acid levels were significantly lower compared to the control group. However, the hepatic thiobarbituric acid- reactive substances, protein oxidation levels and the lipid peroxidation/catalase+glutathione peroxidase (LPO/CAT+GPx ratio were significantly higher in fish transported with both concentrations of the EO, indicating oxidative stress in the liver. In conclusion, considering the hepatic oxidative stress parameters analyzed in the present experiment, the transport of previously sedated silver catfish in water containing 30 or 40 µL L-1 of EO from L. alba is less effective than the use of lower concentrations.

  9. [Metabolomics study of anti-platelet effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by UPLC-MS].

    Science.gov (United States)

    Yang, Qiwei; Yang, Li; Xiong, Aizhen; Wang, Rui; Wang, Zhengtao

    2011-03-01

    To study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method. After administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker. Both of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers. Metabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.

  10. Chemical variability of essential oils of Lippia alba (Miller) N. E. Brown growing in Costa Rica and Argentina.

    Science.gov (United States)

    Ricciardi, Gabriela; Cicció, José F; Ocampo, Rafael; Lorenzo, Daniel; Ricciardi, Armando; Bandoni, Arnaldo; Dellacassa, Eduardo

    2009-06-01

    Lippia alba (Verbenaceae) is a shrub whose essential oil has important biological, pharmacological, and aromatizing properties. The species has a natural range from Central America to Argentina, being cultivated for its commercial value in Brazil, Argentina and Guatemala, and has been introduced into India and Spain. To reach the economic potential of the plant, the present study was aimed at evaluating L. alba for different chemotypes. The composition of the essential oil from two native populations of L. alba, collected from Argentina and two accessions from Costa Rica, were screened by GC and GC-MS. The results obtained led us to adopt the concept of a biodistribution map, as was proposed previously for the species, representing an approach to the natural biological distribution of the species in America based on the chemotypes described and their geographical distribution. Moreover, the biodiversity reported for the species (seven of eight chemotypes described for L. alba are present in Argentina) suggests the southern region of South America as the centre of distribution for L. alba.

  11. Use of cyclodextrins in biotransformation reactions with cell cultures of Morus nigra: biosynthesis of prenylated chalcone isocordoin.

    Science.gov (United States)

    Bolasco, Adriana; Fioravanti, Rossella; Rossi, Francesca; Rossi, Paola; Vitali, Alberto

    2010-06-16

    In vivo biotransformation experiments were performed by using a cell suspension culture of Morus nigra expressing a high PT (prenyltransferase) activity, fed with the target substrate 2',4'-dihydroxychalcone. In order to improve the reaction yields by enhancing the chalcone solubility, three different cyclodextrins have been used to host the substrate. The respective complexes have been studied by means of both spectroscopic and calorimetric techniques (Fourier-transform infrared, 1H-NMR and differential scanning calorimetry) and the solution behaviours have been characterized by solubility phase studies. The hydroxypropyl-beta-cyclodextrin complex was found to be the most suitable for biotransformation, and the reaction of prenylation resulted in a 6-fold higher yield of the final product when compared with the use of the free substrate. The reaction provided as the sole product the 3'-dimethylallyl derivative isocordoin, a biologically active plant compound. The results obtained allow the development of systems based on the use of biofermentors or the use of immobilized cells in order to enhance the biotransformation yields.

  12. Identification and characterization of genes involved in the jasmonate biosynthetic and signaling pathways in mulberry (Morus notabilis)

    Institute of Scientific and Technical Information of China (English)

    Qing Wang; Bi Ma; Xiwu Qi; Qing Guo; Xuwei Wang; Qiwei Zeng; Ningjia He

    2014-01-01

    Jasmonate (JA) is an important phytohormone regulating growth, development, and environmental response in plants, particularly defense response against herbivorous insects. Recently, completion of the draft genome of the mulberry (Morus notabilis) in conjunction with genome sequencing of silkworm (Bombyx mori) provides an opportuni-ty to study this unique plant-herbivore interaction. Here, we identified genes involved in JA biosynthetic and signaling pathways in the genome of mulberry for the first time, with the majority of samples showing a tissue-biased expression pattern. The analysis of the representative genes 12-oxophy-todienoic acid reductase (OPRs) and jasmonate ZIM-domain (JAZs) was performed and the results indicated that the mulberry genome contains a relatively smal number of JA biosynthetic and signaling pathway genes. A gene encoding an important repressor, MnNINJA, was identified as an alternative splicing variant lacking an ethylene-responsive element binding factor-associated amphiphilic repression motif. Having this fundamental information wil facilitate future functional study of JA-related genes pertaining to mulberry-silkworm interactions.

  13. Changes in leaf organisation, photosynthetic performance and wood formation during ex vitro acclimatisation of black mulberry (Morus nigra L.).

    Science.gov (United States)

    Misalová, A; Durkovic, J; Mamonová, M; Priwitzer, T; Lengyelová, A; Hladká, D; Lux, A

    2009-09-01

    Changes in anatomical organisation of the leaf, photosynthetic performance and wood formation were examined to evaluate the temporal and spatial patterns of acclimatisation of micropropagated slow-growing black mulberry (Morus nigra L.) plantlets to the ex vitro environment. Leaf structure differentiation, the rates of net photosynthesis (P(n)), transpiration (E) and stomatal conductance (g(s)), and secondary xylem growth were determined in the course of a 56-day acclimatisation. Differentiation of palisade parenchyma was observed 7 days after transfer. At this stage, the rates of P(n), E and g(s) reached maximum values, after which the rates of all three gas exchange parameters gradually decreased. The highest proportion of woody area occupied by vessels was also observed 7 days after transfer. An important feature of developing woody tissue is the difference in patterns of vessel distribution from the characteristic differentiation patterns of earlywood and latewood vessels in mature wood of ring-porous trees. Vessels with lumen areas over 3000 microm(2) were only differentiated in acclimatised plantlets, whereas vessels in stems sampled on days 0 and 7 had very small lumen areas of up to 560 microm(2). Full acclimatisation, observed 56 days after transfer to the ex vitro environment, was associated with the rapid growth of new in vivo formed leaves, very low rates of E and g(s), and much increased secondary xylem tissue within the stem area.

  14. Sistema reprodutivo do Ipê-Branco: Tabebuia roseo-alba (Ridley Sandwith (Bignoniaceae Breeding system of the White Trumpet Tree: Tabebuia roseo-alba (Ridley Sandwith (Bignoniaceae

    Directory of Open Access Journals (Sweden)

    Gabriel Gandolphi

    2010-09-01

    Full Text Available Estudos sobre sistemas reprodutivos têm indicado o predomínio da autoincompatibilidade de ação tardia (AIT em Bignoniaceae, embora poucas espécies tenham sido investigadas e ocorram outros tipos de sistemas reprodutivos na família. O presente estudo objetivou determinar o sistema reprodutivo de T. roseo-alba através de experimentos de polinizações controladas, análise histológica dos eventos posteriores à polinização, verificação do desenvolvimento in situ dos tubos polínicos e testes de germinação de sementes. Apesar de os tubos polínicos penetrarem e fecundarem a maioria dos óvulos em pistilos autopolinizados, o aborto de 100% dos mesmos foi verificado e, embora sua abscisão tenha ocorrido entre o quarto e o sexto dia após o início da antese, observou-se um ligeiro crescimento dos óvulos e do ovário precedendo a abscisão, porém inferior ao crescimento nos pistilos submetidos à polinização cruzada. A endospermogênese inicial e a formação do tubo proembriônico também foram mais lentas nos pistilos autopolinizados. A longevidade dos pistilos autopolinizados foi maior que a de pistilos não polinizados, e a taxa de germinação de sementes foi de 93%, sendo todas as sementes monoembriônicas. Os resultados demonstram que T. roseo-alba é espécie auto-estéril, destituída de poliembrionia e que apresenta AIT pós-zigótica.Breeding system studies have indicated the predominance of late-acting self-incompatibility (LSI in Bignoniaceae, despite the relatively few species investigated, and the occurrence of other kinds of breeding systems in this family. This study aimed to determine the breeding system in T. roseo-alba by means of controlled experimental pollination, histological analysis of post-pollination events, and studies of pistil longevity, in situ pollen tube growth and seed germination. Despite pollen tube penetration and fertilization of most ovules of selfed pistils, 100% of these pistils aborted

  15. Examination of correlation between histidine and nickel absorption by Morus L., Robinia pseudoacacia L. and Populus nigra L. using HPLC-MS and ICP-MS.

    Science.gov (United States)

    Ozen, Sukran Akkus; Yaman, Mehmet

    2016-08-02

    In this study, HPLC-MS and ICP-MS methods were used for the determination of histidine and nickel in Morus L., Robinia pseudoacacia L., and Populus nigra L. leaves taken from industrial areas including Gaziantep and Bursa cities. In the determination of histidine by HPLC-MS, all of the system parameters such as flow rate of mobile phase, fragmentor potential, injection volume and column temperature were optimized and found to be 0.2 mL min(-1), 70 V, 15 µL, and 20°C, respectively. Under the optimum conditions, histidine was extracted from plant sample by distilled water at 90°C for 30 min. Concentrations of histidine as mg kg(-1) were found to be between 2-9 for Morus L., 6-13 for Robinia pseudoacacia L., and 2-10 for Populus nigra L. Concentrations of nickel were in the ranges of 5-10 mg kg(-1) for Morus L., 3-10 mg kg(-1) for Robinia pseudoacacia L., and 0.6-4 mg kg(-1) for Populus nigra L. A significant linear correlation (r = 0.78) between histidine and Ni was observed for Populus nigra L., whereas insignificant linear correlation for Robinia pseudoacacia L. (r = 0.22) were seen. Limits of detection (LOD) and quantitation (LOQ) were found to be 0.025 mg Ni L(-1) and 0.075 mg Ni L(-1), respectively.

  16. Cloreto de potássio e fosfato de sódio na multiplicação in vitro de amoreira-preta cv. Tupy Effect of KCl and NaH2PO4.H2O on in vitro multiplication of blackberry cv. Tupy

    Directory of Open Access Journals (Sweden)

    Fabíola Villa

    2008-02-01

    Full Text Available A micropropagação da amoreira-preta pode gerar plantas livres de vírus e em curto espaço de tempo. Com o objetivo de aprimorar técnicas de micropropagação de amoreira-preta cv. Tupy (Rubus spp., segmentos nodais com cerca 2 cm e 2 gemas axilares, oriundos de plantas pré estabelecidas in vitro, foram excisados e inoculados em meio de cultura MS, suplementado com diferentes concentrações de fosfato de sódio (0, 125, 250, 500 e 1000 mg L-1 e de cloreto de potássio (0, 125, 250, 500 e 1000 mg L-1. O pH foi ajustado para 5,8 antes da adição de 6 g L-1 de ágar e da autoclavagem a 121ºC e 1 atm por 20 minutos. Após a inoculação, os explantes foram transferidos para sala de crescimento a 25 ± 1ºC, irradiância de 35 mmol m-2 s-1 e fotoperíodo de 16 horas, onde permaneceram por 60 dias. O delineamento experimental utilizado foi inteiramente casualisado, utilizando-se de quatro repetições constituídas de três tubos de ensaio contendo um explante cada. O número de brotos e o comprimento da parte aérea das plantas foi menor em função de maiores concentrações de cloreto de potássio. Melhores resultados foram obtidos na ausência de KCl e na presença de fosfato de sódio, principalmente para comprimento e matéria fresca da parte aérea.The micropropagation of blackberry can generate virus-free plants in short time. In order to improve micropropagation techniques of blackberry cv. Tupy (Rubus spp., nodal segments with 2 cm length and 2 axillary buds originating from plants in vitro were excised and inoculated in MS culture medium, supplemented with different concentrations of sodium phosphate (0, 125, 250, 500 and 1000 mg L-1 and potassium chloride (0, 125, 250, 500 and 1000 mg L-1. The pH was adjusted to 5.8 before the addition of 6 g L-1 agar and sterilization at 121ºC and 1 atm for 20 minutes. After the inoculation, the explants were transferred to growth room at 25 ± 1ºC, 35 mmol m-2 s-1 irradiance and photoperiod of

  17. Uncorrected land-use planning highlighted by flooding: the Alba case study (Piedmont, Italy)

    Science.gov (United States)

    Luino, F.; Turconi, L.; Petrea, C.; Nigrelli, G.

    2012-07-01

    Alba is a town of over 30 000 inhabitants located along the Tanaro River (Piedmont, northwestern Italy) and is famous for its wine and white truffles. Many important industries and companies are based in Alba, including the famous confectionery group Ferrero. The town suffered considerably from a flood that occurred on 5-6 November 1994. Forty-eight percent of the urban area was inundated, causing severe damage and killing nine people. After the flood, the Alba area was analysed in detail to determine the reasons for its vulnerability. Information on serious floods in this area since 1800 was gathered from official records, state technical office reports, unpublished documents in the municipal archives, and articles published in local and national newspapers. Maps, plans and aerial photographs (since 1954) were examined to reconstruct Alba's urban development over the last two centuries and the planform changes of the Tanaro River. The results were compared with the effects of the November 1994 flood, which was mapped from aerial photographs taken immediately after the flood, field surveys and eyewitness reports. The territory of Alba was subdivided into six categories: residential; public service; industrial, commercial and hotels; sports areas, utilities and standards (public gardens, parks, athletics grounds, private and public sport clubs); aggregate plants and dumps; and agriculture and riverine strip. The six categories were then grouped into three classes with different flooding-vulnerability levels according to various parameters. Using GIS, the three river corridors along the Tanaro identified by the Autorità di Bacino del Fiume Po were overlaid on the three classes to produce a final map of the risk areas. This study shows that the historic floods and their dynamics have not been duly considered in the land-use planning of Alba. The zones that were most heavily damaged in the 1994 flood were those that were frequently affected in the past and sites of

  18. Uncorrected land-use planning highlighted by flooding: the Alba case study (Piedmont, Italy

    Directory of Open Access Journals (Sweden)

    F. Luino

    2012-07-01

    Full Text Available Alba is a town of over 30 000 inhabitants located along the Tanaro River (Piedmont, northwestern Italy and is famous for its wine and white truffles. Many important industries and companies are based in Alba, including the famous confectionery group Ferrero.

    The town suffered considerably from a flood that occurred on 5–6 November 1994. Forty-eight percent of the urban area was inundated, causing severe damage and killing nine people. After the flood, the Alba area was analysed in detail to determine the reasons for its vulnerability.

    Information on serious floods in this area since 1800 was gathered from official records, state technical office reports, unpublished documents in the municipal archives, and articles published in local and national newspapers. Maps, plans and aerial photographs (since 1954 were examined to reconstruct Alba's urban development over the last two centuries and the planform changes of the Tanaro River.

    The results were compared with the effects of the November 1994 flood, which was mapped from aerial photographs taken immediately after the flood, field surveys and eyewitness reports.

    The territory of Alba was subdivided into six categories: residential; public service; industrial, commercial and hotels; sports areas, utilities and standards (public gardens, parks, athletics grounds, private and public sport clubs; aggregate plants and dumps; and agriculture and riverine strip. The six categories were then grouped into three classes with different flooding-vulnerability levels according to various parameters. Using GIS, the three river corridors along the Tanaro identified by the Autorità di Bacino del Fiume Po were overlaid on the three classes to produce a final map of the risk areas.

    This study shows that the historic floods and their dynamics have not been duly considered in the land-use planning of Alba. The zones that were most heavily damaged in the 1994 flood were

  19. Optimization of in vitro and ex vitro regeneration and micromorphological studies in Basella alba L.

    Science.gov (United States)

    Shekhawat, Mahipal S; Manokari, M

    2016-10-01

    The optimum concentrations of the plant hormones for in vitro regeneration and subsequent effect of auxins on rooting (in vitro and ex vitro) of shoots of Basella alba L. have been investigated in present study. Nodal shoot segments were used as explants to initiate the cultures. The bud breaking from explants was observed within 1 week of incubation on agar gelled Murashige and Skoog's (MS) medium. Multiple axillary shoots (7.30 ± 0.56 shoots per explant) were induced on MS medium supplemented with 2.0 mg/L 6-benzylaminopurine (BAP). The shoots were multiplied (maximum 17.10 ± 0.44 shoots per explant) on the same medium fortified with 0.5 mg/L each of BAP and Kin (Kinetin) +0.1 mg/L IAA. These shoots were excised and rooted in vitro (10.73 ± 0.92 roots per shoot) on half-strength MS medium augmented with 2.0 mg/L indole-3 butyric acid (IBA). Hundred percentage success rates have been achieved by ex vitro rooting of the in vitro regenerated shoots with IBA at 300 mg/L. The in vitro and ex vitro rooted shoots were acclimatized in greenhouse and subsequently transferred to the natural field conditions where 100 % survival rate was reported. The ex vitro rooting method was found more advantageous than in vitro rooting in terms of time, energy and survival percentage of B. alba. A comparative foliar micromorphological study of B. alba was conducted to understand the micromorphological changes in plants while shifting from in vitro to the in vivo conditions in terms of variations in stomatal index, venation pattern and vein density, and the arrangement of crystals. The study could help in understanding the response of in vitro raised plants towards in vivo environment.

  20. Archaeal chromatin proteins histone HMtB and Alba have lost DNA-binding ability in laboratory strains of Methanothermobacter thermautotrophicus.

    Science.gov (United States)

    Sandman, Kathleen; Louvel, Hélène; Samson, Rachel Y; Pereira, Suzette L; Reeve, John N

    2008-11-01

    Alignments of the sequences of the all members of the archaeal histone and Alba1 families of chromatin proteins identified isoleucine residues, I19 in HMtB and I39 in MtAlba, in Methanothermobacter thermautotrophicus, at locations predicted to be directly involved in DNA binding. In all other HMfB family members, residue 19 is an arginine (R19), and either arginine or lysine is present in almost all other Alba1 family members at the structural site equivalent to I39 in MtAlba. Electrophoretic mobility shift assays revealed that recombinant HMtB and MtAlba do not bind DNA, but variants constructed with R19 and R39, respectively, bound DNA; and whereas MtAlba(I19) did not bind RNA, MtAlba(R19) bound both single stranded RNA and tRNA. Amplification and sequencing of MT0254 (encodes HMtB) and MT1483 (encodes MtAlba) from several Methanothermobacter thermautotrophicus lineages has revealed that HMtB and MtAlba had arginine residues at positions 19 and 39, respectively, in the original isolate and that spontaneous mutations must have occurred, and been fixed, in some laboratory lineages that now have HMtB(I19) and MtAlba(I39). The retention of these variants suggests some continuing functions and fusion of the HMtB(I19) sequence to HMtA2 resulted in a protein that folds to form a histone fold heterodimer that binds and compacts DNA. The loss of DNA binding by HMtB(I19) does not therefore prevent HMtB from participating in DNA interactions as one partner of an archaeal histone heterodimer.

  1. An Analysis on Phylogenetic Relationship and Divergence Time of Yunnan Guangye Sang and Qinzhou Changguo Sang in Morus Genus Based on ITS Sequence%基于ITS分析云南光叶桑和钦州长果桑在桑属中的亲缘关系及分化时间

    Institute of Scientific and Technical Information of China (English)

    陈仁芳; 陈祥平; 范小敏; 陈家元; 刘泽听; 虞志伟

    2012-01-01

    云南光叶桑、钦州长果桑在形态分类学上均属同一桑种Morus macroura.通过PCR扩增2份地方品种资源材料的ITS序列并分析序列差异及构建系统进化树,阐明各自在桑属中的遗传分化关系.2份材料的ITS序列长度均为576 bp,其中:云南光叶桑的G+C含量为59.90%,碱基序列45位A变G,560位T变G,与昆明奶桑(Morus macrou,)、荥经川桑(Morus notabilis)、云南毛叶奶桑(Morus macroura var.mawa)、云南长穗桑(Morus wittiorum)、云南奶桑(Morus macroura)、雅安华桑(Morus cathayana)同在第Ⅰ类群,有较近的亲缘关系;钦州长果桑的G+C含量为59.72%,碱基序列45位为A,560位T变G,与云南华桑(Morus cathayana)同在第Ⅱ类群,有较近的亲缘关系.应用松散分子钟方法估算云南光叶桑与荥经川桑、雅安华桑、云南长穗桑的分化时间为8.23 Ma,钦州长果桑与云南华桑的分化时间为9.67 Ma,二者起源的地质年代是新第三纪中新世与上新世之交,是为了抵御地球寒冷、旱化,向南、向山地迁移形成的物种.%Yunnan Guangye Sang and Qinzhou Changguo Sang belong to the same mulberry species, Morus macroura, in morphological taxonomy. In this study, the ITS sequences of both local variety materials were PCR amplified, analyzed on their sequence difference, and used to construct phylogenetic trees for elucidation of their inheritance and divergence behavior in the Morus genus. The ITS sequence of both materials is 576 bp. However, the G +C content in Yunnan Guangye Sang is 59. 90%, and A has been replaced by G at nucleotide 45 and T by G at nucleotide 560. It has close phylogenetic relationship with Kunming Nai Sang (Morus macroura), Yingjing Chuan Sang (Morus notabilis), Yunnan Maoyenai Sang (Morus macroura var. Mawa), Yunnan Changsui Sang ( Morus wittiorum), Yunnan Nai Sang ( Morus macroura), and Ya'an Hua Sang (Morus cathayana), all of which belong to group | in the constructed phylogenetic tree. The G + C

  2. Electron probe microanalysis of fluorine in polluted needle sections of Abies alba

    Energy Technology Data Exchange (ETDEWEB)

    Garrec, J.P.; Lhoste, A.M.

    1976-01-01

    After fumigation with hydrogen fluoride, sections of needles of Abies alba were studied by electron probe microanalysis. The levels of fluoride vary in different tissues: most fluoride was found in the spongy mesophyll and the palisade tissues as a function of the distance from the cells of entry and the paths of translocation of fluoride. These results support the view that the differential responses of various tissues of a leaf to fumigation with HF depend on the localization of the tissues rather than on a specific response by a particular tissue to fluoride. 15 references, 6 figures.

  3. Pityriasis Alba--Common Disease, Enigmatic Entity: Up-to-Date Review of the Literature.

    Science.gov (United States)

    Miazek, Nina; Michalek, Irmina; Pawlowska-Kisiel, Malgorzata; Olszewska, Malgorzata; Rudnicka, Lidia

    2015-01-01

    Pityriasis alba (PA) is a skin disorder that affects children and adolescents. Although it is common worldwide, its incidence is markedly higher in darker skin phototypes. Its characteristic features include an extended, multistage course and spontaneous remissions and recurrences. Preceded by erythematous changes, patches of hypopigmented skin of up to a few centimeters in diameter appear on the upper body. Pruritus may accompany it. Even though its etiology is unknown, possible reported triggering factors include sunlight, beauty treatments, and microorganisms, among others. Calcineurin inhibitors play the most crucial role in PA pharmacotherapy. PA often coexists with atopic dermatitis and is considered one of its milder forms.

  4. Morphology and histochemistry of glandular trichomes of Orobanche alba Stephan ex Willd

    Directory of Open Access Journals (Sweden)

    Aneta Sulborska

    2014-04-01

    Full Text Available Orobanche alba Stephan ex Willd is an achlorophyllous root parasite rare in Poland. It prefers dry and sunny slopes, xerothermic grasslands and pastures, mountain pastures, light scrubs, and rock fissures and ledges. The hosts of O. alba include Thymus polytrichus A. ern. ex Borbás, Clinopodium vulgare L. and Origanum vulgare L. The tick and fleshy 10-70 cm high stem in this species bears an inflorescence composed of zygomorphic, white or yellow “spotted” flowers covered by purple glandular trichomes. Glandular trichomes of this type are also borne on other parts of the plant, i.e. on the stem, scaly leaves, sepals, filaments, and the style. The secondary metabolites secreted by the glandular trichomes are related to defense of plants against the attack of herbivores and pathogens or act as attractants to pollinators or for fruit dispersal. The micromorphology and histochemistry of the glandular trichomes in O. alba were examined using scanning electron and light microscopes. In order to determine the type of secondary metabolites produced by the trichomes, the flowing histochemical assays were used: Sudan III and neutral red for detection of lipophilic compounds, IKI for detection of starch, and FeCl3 for detection of phenolic compounds. The peltate glandular trichomes of O. alba were characterised by a varied length (0.15‑0.48 mm and different activity phases. The trichome was composed of one larger basal epidermal cell, 1-3 hyaline stalk cells with a striated cuticle, a neck cell with a smooth cuticle on the surface, and a globose head formed of 8-18 secretory cells arranged in a circle. Many stalk cells of the trichomes, particularly those located on the corolla, contained anthocyanins, which give the trichomes dark carmine colour. In turn, the colour of the heads was dependent on trichome age: the heads were brown in older trichomes and yellow in younger hairs. Secretion was produced by both young and older trichomes. It penetrated

  5. Zu den Quinquennales D.D. in den fasti et alba augustalium aus ostia

    OpenAIRE

    Abramenko, A.

    1992-01-01

    En los Fasti y Alba Augustalium aparece regularmente tras el título de los quinquenales la abreviatura dd. Si bien existe acuerdo de que ésta no deba, como se suele, ser interpretada d(ecreto) d(ecurionum), sino d(ono) d(ato), esta última propuesta había permanecido hasta ahora sin prueba definitiva. Sobre la base de otros usos de la abreviatura dd se verá aquí que la interpretación d(ono) d(ato) es la justa, exponiéndose las razones por las cuales se utiliza esta fórmula en Ostia.

  6. Comparison of the pathogenicity, growth, sporulation and morphology of Pezicula alba Guthrie (Gloeosporium album Osterw.

    Directory of Open Access Journals (Sweden)

    H. Borecka

    2013-12-01

    Full Text Available Sixteen isolates of Pezicula alba Guthr. were examined. The intensity of growth on various media, pathogenicity to apple fruits and twigs, colour of cultures and size of conidia were measured. Some isolates are pathogenic to fruits, some others to twigs; one isolate (only no. 19 is pathogenic to both twigs and fruits; many isolates are not - pathogenic at all. Culture growth, sporulation and size of conidia are not correlated with the pathogenicity of the isolate. The mean size of conidia is 21.29µm x 3.48µm.

  7. Fernando Alba: espacio escultórico, espacio arquitectónico y espacio urbano

    OpenAIRE

    Martínez Sierra, Jovino

    2012-01-01

    El tema objeto de estudio de esta Tesis Doctoral es el análisis de la obra del escultor asturiano Fernando Alba, uno de los escultores contemporáneos de Asturias que cuenta con una trayectoria de más de cuarenta años de actividad ininterrumpida dentro de la plástica y uno de los artistas que ha dejado una muestra importante por número y calidad de su actividad dentro y fuera de la región. El estudio del conjunto de su producción resulta fundamental para el conocimiento del arte asturiano a...

  8. Ventajas competitivas de la empresas grannacionales como modelo empresarial del ALBA-TCP

    OpenAIRE

    Pinho De Oliveira, María Fátima

    2012-01-01

    En la presente investigación, se pretende analizar algunas de las ventajas competitivas de las empresas grannacionales como modelos empresariales dentro del marco del ALBA-TCP, motivado a que debido a la globalización como fenómeno mundial, aunado con las condiciones económicas de países asociados, se ven afectados por las competencias económicas de compañías a nivel mundial. Con la integración económica desarrollada en Europa con el fin de alcanzar la paz, el progreso y ocupar la mejor posic...

  9. De novo Transcriptome Analysis of Sinapis alba in Revealing the Glucosinolate and Phytochelatin Pathways

    Science.gov (United States)

    Zhang, Xiaohui; Liu, Tongjin; Duan, Mengmeng; Song, Jiangping; Li, Xixiang

    2016-01-01

    Sinapis alba is an important condiment crop and can also be used as a phytoremediation plant. Though it has important economic and agronomic values, sequence data, and the genetic tools are still rare in this plant. In the present study, a de novo transcriptome based on the transcriptions of leaves, stems, and roots was assembled for S. alba for the first time. The transcriptome contains 47,972 unigenes with a mean length of 1185 nt and an N50 of 1672 nt. Among these unigenes, 46,535 (97%) unigenes were annotated by at least one of the following databases: NCBI non-redundant (Nr), Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, Gene Ontology (GO), and Clusters of Orthologous Groups of proteins (COGs). The tissue expression pattern profiles revealed that 3489, 1361, and 8482 unigenes were predominantly expressed in the leaves, stems, and roots of S. alba, respectively. Genes predominantly expressed in the leaf were enriched in photosynthesis- and carbon fixation-related pathways. Genes predominantly expressed in the stem were enriched in not only pathways related to sugar, ether lipid, and amino acid metabolisms but also plant hormone signal transduction and circadian rhythm pathways, while the root-dominant genes were enriched in pathways related to lignin and cellulose syntheses, involved in plant-pathogen interactions, and potentially responsible for heavy metal chelating, and detoxification. Based on this transcriptome, 14,727 simple sequence repeats (SSRs) were identified, and 12,830 pairs of primers were developed for 2522 SSR-containing unigenes. Additionally, the glucosinolate (GSL) and phytochelatin metabolic pathways, which give the characteristic flavor and the heavy metal tolerance of this plant, were intensively analyzed. The genes of aliphatic GSLs pathway were predominantly expressed in roots. The absence of aliphatic GSLs in leaf tissues was due to the shutdown of BCAT4, MAM1, and CYP79F1 expressions. Glutathione was extensively

  10. De novo transcriptome analysis of Sinapis alba in revealing the glucosinolate and phytochelatin pathways

    Directory of Open Access Journals (Sweden)

    Xiaohui eZhang

    2016-03-01

    Full Text Available Sinapis alba is an important condiment crop and can also be used as a phytoremediation plant. Though it has important economic and agronomic values, sequence data and the genetic tools are still rare in this plant. In the present study, a de novo transcriptome based on the transcriptions of leaves, stems and roots was assembled for S. alba for the first time. The transcriptome contains 47,972 unigenes with a mean length of 1,185 nt and an N50 of 1,672 nt. Among these unigenes, 46,535 (97% unigenes were annotated by at least one of the following databases: NCBI non-redundant (Nr, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG pathway, Gene Ontology (GO, and Clusters of Orthologous Groups of proteins (COGs. The tissue expression pattern profiles revealed that 3,489, 1,361 and 8,482 unigenes were predominantly expressed in the leaves, stems and roots of S. alba, respectively. Genes predominantly expressed in the leaf were enriched in photosynthesis- and carbon fixation-related pathways. Genes predominantly expressed in the stem were enriched in not only pathways related to sugar, ether lipid and amino acid metabolisms but also plant hormone signal transduction and circadian rhythm pathways, while the root-dominant genes were enriched in pathways related to lignin and cellulose syntheses, involved in plant-pathogen interactions, and potentially responsible for heavy metal chelating and detoxification. Based on this transcriptome, 14,727 simple sequence repeats (SSRs were identified, and 12,830 pairs of primers were developed for 2,522 SSR-containing unigenes. Additionally, the glucosinolate (GSL and phytochelatin metabolic pathways, which give the characteristic flavor and the heavy metal tolerance of this plant, were intensively analyzed. The genes of aliphatic GSLs pathway were predominantly expressed in roots. The absence of aliphatic GSLs in leaf tissues was due to the shutdown of BCAT4, MAM1 and CYP79F1 expressions. Glutathione was

  11. The Prokinetic, Laxative, and Antidiarrheal Effects of Morus nigra: Possible Muscarinic, Ca(2+) Channel Blocking, and Antimuscarinic Mechanisms.

    Science.gov (United States)

    Akhlaq, Anam; Mehmood, Malik Hassan; Rehman, Abdul; Ashraf, Zohaib; Syed, Sadia; Bawany, Samira Ahmed; Gilani, Anwarul-Hassan; Ilyas, Maimoona; Siddiqui, Bina Shaheen

    2016-08-01

    Morus nigra Linn. (black mulberry) is used in gastrointestinal ailments. This study demonstrates gut modulatory properties of M. nigra. The prokinetic, laxative, and antidiarrheal activities of M. nigra were assessed in mice, while isolated rabbit jejunum and guinea-pig ileum were used to explore insight into mechanism(s). At 30 and 70 mg/kg, the crude extract of M. nigra (Mn.Cr) exhibited atropine-sensitive prokinetic and laxative effects, similar to carbachol (CCh). While at higher doses (100, 300, and 500 mg/kg), Mn.Cr offered protection against castor oil-induced diarrhea. In rabbit jejunum, Mn.Cr and its chloroform fraction inhibited CCh-induced contractions more potently compared with high K(+) (80 mm). Conversely, petroleum fraction was more potent against high-K(+) -induced contractions. At 0.01 mg/mL, Mn.Cr caused a parallel shift in acetylcholine concentration-response curves (CRCs) followed by a non-parallel shift at 0.03 mg/mL, similar to dicyclomine. At further tested concentrations, Mn.Cr (0.1 and 0.3 mg/mL) and petroleum fraction suppressed Ca(2+) CRCs, similar to verapamil. In guinea-pig ileum, Mn.Cr, its aqueous and ethyl acetate fractions exhibited atropine-sensitive gut stimulant activity along with additional uncharacterized excitatory response in the aqueous fraction only. These results suggest that black mulberry possesses prokinetic, laxative, and antidiarrheal effects, putatively mediated through cholinomimetic, antimuscarinic, and Ca(2+) antagonist mechanisms, respectively. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  12. A novel lectin (Morniga M) from mulberry (Morus nigra) bark recognizes oligomannosyl residues in N-glycans.

    Science.gov (United States)

    Wu, Albert M; Wu, June H; Singh, Tanuja; Chu, Kang-Chuang; Peumans, Willy J; Rougé, Pierre; Van Damme, Els J M

    2004-01-01

    Morniga M is a jacalin-related and mannose-specific lectin isolated from the bark of the mulberry (Morus nigra). In order to understand the function and application of this novel lectin, the binding property of Morniga M was studied in detail using an enzyme-linked lectinosorbent assay and lectin-glycan inhibition assay with extended glycan/ligand collection. From the results, it was found that the di-, tri-, and oligomannosyl structural units of N-glycans such as those of the bovine alpha1-acid glycoprotein (gp) and lactoferrin were the most active gps, but not the O-glycans or polysaccharides including mannan from yeast. The binding affinity of Morniga M for ligands can be ranked in decreasing order as follows: gps carrying multiple N-glycans with oligomannosyl residues > N-glycopeptide with a single trimannosyl core > Tri-Man oligomer [Man alpha1-->6(Man alpha1-->3) Man], Penta-Man oligomer [Man alpha1-->6(Man alpha1-->3)Man alpha1-->6(Man alpha1-->3) Man] > or = Man alpha1-->2, 3 or 6 Man > Man > GlcNAc, Glc > L-Fuc, Gal, GalNAc (inactive), demonstrating the unique specificity of this lectin that may not only assist in our understanding of cell surface carbohydrate ligand-lectin recognition, but also provide informative guidelines for the application of this structural probe in biotechnological and clinical regimens, especially in the detection and purification of N-linked glycans. 2004 National Science Council, ROC and S. Karger AG, Basel

  13. Recognition profile of Morus nigra agglutinin (Morniga G) expressed by monomeric ligands, simple clusters and mammalian polyvalent glycotopes.

    Science.gov (United States)

    Singh, Tanuja; Wu, June H; Peumans, Willy J; Rougé, Pierre; Van Damme, Els J M; Wu, Albert M

    2007-01-01

    The carbohydrate binding properties of a novel member of the subfamily of galactose-specific jacalin-related lectin isolated from the bark of black mulberry (Morus nigra) (Morniga G) was studied in detail by enzyme-linked lectinosorbent and inhibition assays using panels of monomeric saccharides, mammalian polyvalent glycotopes and polysaccharides. Among the natural glycans tested for lectin binding, Morniga G reacted best with glycoproteins (gps) presenting a high density of tumor-associated carbohydrate antigens Tn (GalNAcalpha1-Ser/Thr) and Talpha (Galbeta1-3GalNAcalpha1-). Their reactivities, on a nanogram basis, were up to 72.5, 3.9x10(3), 6.0x10(3), 8.8x10(3) and 2.9x10(4) times higher than that of Tn-containing glycopeptides (M.W.Man/Glc, GlcNAc and lFuc; (ii) the mammalian glycotope specificity is Talpha1-benzyl>T>Tn>GalNAcbeta1-3Gal (P), while B/E (Galalpha1-3/4Gal), I/II (Galbeta1-3/4GlcNAc), S (GalNAcbeta1-4Gal), F/A (GalNAcalpha1-3GalNAc/Gal) and L (Galbeta1-4Glc) are inactive; (iii) the most active ligand is T/Tn; (iv) simple clustered Tn or triantennary N-glycans with II termini (Tri-II) have limited impact; (v) high-density polyvalent glycotopes play a prominent role for enhancing Morniga G reactivity. These results provide evidence for the binding of this lectin to dense cell surface T/Tn glycoconjugates and facilitate future usage of this lectin in biotechnological and medical applications.

  14. A novel bioactive chalcone of Morus australis inhibits tyrosinase activity and melanin biosynthesis in B16 melanoma cells.

    Science.gov (United States)

    Takahashi, Makoto; Takara, Kensaku; Toyozato, Tomonao; Wada, Koji

    2012-01-01

    The methanol extract of Morus australis (shimaguwa) acts as a whitening agent due to the inhibition of tyrosinase activity. In order to explore the mechanism(s) of the whitening action, constituents of the 95% methanol extract from the dried stems of shimaguwa were isolated and their skin-whitening capacity was examined. Bioassay-guided fractionation of the methanol soluble extract of shimaguwa led to the isolation of 2, 4, 2', 4'-hydroxycalcone (chalcone 1) and three analogues of chalcone 1 with 3'-substituted resorcinol moieties (chalcones 2-4). Chalcone derivative 4 proved to be a novel compound and was fully characterized. Chalcones 1-4 were evaluated for inhibition activity on mushroom tyrosinase using L-tyrosine as the substrate. The parent chalcone 1 was a highly effective inhibitor of tyrosinase activity (IC₅₀ = 0.21 μM) compared to arbutin (IC₅₀ = 164 μM). Compared to chalcone 1, chalcones 2 and 3, which possess 3'-substituted isoprenyl or bulky 2-benzoylbiphenyl, showed significantly decreased tyrosinase activity, while chalcone 4, possessing 3'-substituted 2-hydroxy-1-pentene group, showed slightly increased activity.The effects of chalcones 1-4 on melanin synthesis, without affecting cell growth, were assayed in melanin-producing B16 murine melanoma cells. Chalcone 3 significantly reduced cell viability before reaching the IC₅₀ value for melanin synthesis. In contrast, the inhibitory effects of chalcones 1, 2 and 4 were more than 100-fold greater than that of arbutin, with little or no cytotoxicity. More significantly, chalcone 2, which exhibited less tyrosinase inhibitory activity compared to the parent chalcone 1, showed the highest inhibition of melanin synthesis in B16 cells among the chalcones tested. Accordingly, chalcones 1 and 2, and the novel chalcone 4 might be the active components responsible for the whitening ability of shimaguwa. Moreover, whitening ability was not exclusively due to tyrosinase inhibition.

  15. Pharmacological effects of ethanol extract of Egyptian Artemisia herba-alba in rats and mice

    Institute of Scientific and Technical Information of China (English)

    Heba Mohammed Ibrahim Abdallah; Nawal E.L. Sayed Gomaa

    2016-01-01

    Objective: To investigate some pharmacological effects including gastroprotective, anti-inflammatory, analgesic, antipyretic and in vitro antioxidant effects of Artemisia herba-alba extract in different experimental models. Methods: Inflammation was induced in rat paw by subcutaneous injection of 1% (v/v) carrageenan solution. Writhes was induced in mice by intraperitoneal injection of 0.6%(v/v) acetic acid solution. Pyrexia was induced using Brewer's yeast suspension. Gastric lesion was induced in rats by oral administration of 99% ethanol. The anti-inflammatory, analgesic, antipyretic and gastroprotective activities of Artemisia herba-alba extract were investigated respectively. In vitro antioxidant effect was investigated using DPPH free radical. Results: The plant extract showed anti-inflammatory effect in carrageenan-induced paw edema in rats, analgesic effect against acetic acid-induced writhing, and antipyretic ac-tivity in Brewer's yeast model of pyrexia. Besides, it was shown to be a gastroprotective agent against ethanol-induced gastric ulcers. The plant also exhibited a free radical scavenging potential in an in vitro antioxidant study using DPPH. Conclusions: The results validate the use of the investigated plant in traditional medicine for different ailments.

  16. Intergeneric Somatic Hybridization Between Brassica napus L. And Sinapis alba L.

    Institute of Scientific and Technical Information of China (English)

    You-Ping WANG; Karin SONNTAG; Eicke RUDLOFF; Jian-Min CHEN

    2005-01-01

    Electrically induced protoplast fusion was used to produce somatic hybrids between Brassica napus L. and Sinapis alba L. Seven hybrids were obtained and verified by the simple sequence repeat and cleaved amplified polymorphic sequence analysis of the genefael, indicating that the characteristic bands from S. alba were present in the hybrids. The hybridity was also confirmed by chromosome number counting because the hybrids possessed 62 chromosomes, corresponding to the sum of fusion-parent chromosomes. Chromosome pairing at meiosis was predominantly normal, which led to high pollen fertility,ranging from 66% to 77%. All hybrids were grown to full maturity and could be fertilized and set seed after self-pollination or back-crosses with B. napus. The morphology of the hybrids resembled characteristics from both parental species. An analysis of the fatty acid composition in the seeds of F1 plants was conducted and the seeds were found to contain different amounts of erucic acid, ranging from 11.0% to 52.1%.

  17. Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen infected with Aeromonassp.

    Directory of Open Access Journals (Sweden)

    FERNANDO J. SUTILI

    2015-03-01

    Full Text Available In vitro and in vivo activity of the Lippia alba essential oil (EO against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC and a minimum bactericidal concentration (MBC of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonassp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen (7.50 ± 1.85 g and 10.0 ± 1.0 cm with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10: untreated fish (negative control, 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1 was greater than 90%.

  18. Seasonal variability of the antimicrobial activity of the essential oil of Lippia alba

    Directory of Open Access Journals (Sweden)

    Terezinha Feitosa Machado

    Full Text Available Lippia alba is a shrub of the Verbenaceae family, popularly known as lemon balm. It's essential oil is said to have broad-spectrum antimicrobial activity whose effectiveness varies according to its composition, which is influenced by both biotic and abiotic factors. The present study aimed to evaluate the antimicrobial effectiveness of oil taken from the leaves of L. alba collected at different times of the year, against microorganisms of importance in food. The oil, obtained by hydrodistillation, was tested against standard cultures of Pseudomonas aeruginosa, Salmonella choleraesuis, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Listeria innocua using agar diffusion techniques. The different sampling times had a big influence on the efficiency of the oil, which varied in relation to each microbial species. From December to February the best response was against S. aureus. Against L. monocytogenes, the oil was effective from June to August, while for L. innocua, the oil presented the same response for three periods of the year, differing only from March to May. Correlation of meteorological data for 2010 makes it possible to verify that characteristics typical of the period from December to February in the town of Paraipaba, in the Brazilian state of Ceará (high temperatures, long periods of sunshine and low rainfall favoured the action of the oil against S. aureus. In turn, from June to August, seasonal conditions enhanced the action of the oil against L. monocytogenes and L. innocua.

  19. Selection of new clones of linalool chemotype from genetic recombination in Lippia alba

    Directory of Open Access Journals (Sweden)

    Elcio Rodrigo Rufino

    2012-01-01

    Full Text Available The aromatic and medicinal species Lippia alba is vigorous and rugged native to the South America (Atlantic Rainforest. Because it is an allogamous and self-incompatible species, natural populations have high morphological and chemical variability. This work had as objective to conduct a preliminary screening to identify new promising clones from a novel (recombinant base population of Lippia alba with regard to its agronomic and phytochemical traits, using the linalool oil or chemotype as model. The two superior linalool clones, obtained by collection, were used as controls. Traits evaluated included: dry mass of leaves (DML, oil yield percentage (EOY%, oil production per plant (OP, and linalool percentage (LN%. Forty linalool chemotype clones were evaluated in three experiments, in a random block design with four replicates and four cuttings (clones per plot. Besides means comparisons, multivariate analysis was used in order to aid in the preliminary selection of clones. There were positive correlations from moderate to strong for DML vs. EOY%, OP vs. EOY% and DML vs. OP. Linalool clones superior or similar to both controls were identified for the DML, EOY%, OP, and LN% traits (univariate analyses, aimed at further validating experimentation. Five distinct groups were defined in the cluster analysis (UPGMA, each containing subgroups as well.

  20. Reproductive isolation between sympatric sister species, Mussaenda kwangtungensis and M. pubescens var. alba

    Institute of Scientific and Technical Information of China (English)

    Zhonglai Luo; Tingting Duan; Shuai Yuan; Shi Chen; Xiufeng Bai; Dianxiang Zhang

    2015-01-01

    Reproductive isolation defines the biological species concept and plays a key role in the formation and maintenance of species. The relative contributions of different isolating stages has been suggested to be closely associated with phylogenetic relatedness. Few studies have focused on the relative contributions of pre- versus post-zygotic mechanisms, and even fewer have been conducted under strict phylogenetic frameworks. Pre-and post-zygotic reproductive isolation stages have been investigated in the sister species Mussaenda kwangtungensis and M. pubescens var. alba. The two species have partly overlapping distribu-tion ranges and flowering times, while the principal pollinators differed strikingly for them, demonstrating strong pre-zygotic isolations. Natural hybrids were detected by simple sequence repeat markers and their maternal parents were identified based on chloroplast gene sequen-ces. Five out of 81 individuals were suggested to be hybrids that fall into the categories F2, BC1, and BC2 by the NewHybrids analysis. Interspecific crossings resulted in significantly reduced fruit set and seed germination rates. Phylogenetic analysis revealed short Kimura-2-parameter distance between M. kwangtungensis and M. pubescens var. alba. These findings strongly supported the hypothesis that for species with a closer phylogenetic relationship, pre-zygotic isolation plays an important part in limiting gene exchange in sympatric areas.

  1. Remove of lead from the polluted water by phytoremediation technique (Eclipta Alba plant

    Directory of Open Access Journals (Sweden)

    Mohamad J. Noufal, Zena A. Maalla, Dareen J. Noufal, Ali A. Hossean

    2017-01-01

    Full Text Available One of the major environmental problems is the pollution of water and soils by toxic heavy metals. The aim of current study was estimation the ability of Eclipta alba plant for the removal of (Pb from the polluted water by phytoremediation technique and study the effect of a number of parameters(Pb concentration, contact time and pH values. The plant showed higher removal efficiency (99.2% after 7 days of treatment at 50 ppm. The best removal efficiency of Pb 98.95% at 100 ppm was occurred at pH7. The root ability to accumulate Pb was more than the shoot ability, after 7 days treatment of water and pH=7, they were 2852.5 mg/kg, 2497.5 mg/kg and 502.5 mg/kg in roots, stems and leaves respectively. These results showed that Eclipta Alba can be used in biological treatment of polluted water.

  2. New diketopiperazine derivatives from a deep-sea-derived Nocardiopsis alba SCSIO 03039.

    Science.gov (United States)

    Zhang, Qingbo; Li, Sumei; Chen, Yuchan; Tian, Xinpeng; Zhang, Haibo; Zhang, Guangtao; Zhu, Yiguang; Zhang, Si; Zhang, Weimin; Zhang, Changsheng

    2013-01-01

    The strain SCSIO 03039 was isolated from a sediment sample in the Indian Ocean and was characterized as a Nocardiopsis alba species on the basis of its 16S rRNA gene sequence. Seven diketopiperazines (DKPs), including two new DKPs nocazines D (2a) and E (2b), and five known DKPs (1, 3-6), were isolated from N. alba SCSIO 03039, along with two known compounds 2-methoxy-1,4-naphthoquinone (7) and 1-hydroxy-4-methoxy-2-naphthoic acid (8). Their structures were elucidated by mass and NMR spectroscopic analyses. The structure of methoxyneihumicin (1), previously proposed in a conference poster lacking publicly available experimental data, was validated for the first time by detailed NMR analyses and X-ray diffraction study. The two enantiomers nocazines D (2a) and E (2b) were isolated as a mixture. Compounds 3 and 4 were only known as synthetic compounds before. Methoxyneihumicin (1) exhibited in vitro cytotoxicities against MCF-7 and SF-268 with IC₅₀ values of 4.6 and 12.7 μM, respectively, better than those of 6 (22.0 and 20.6 μM). The other compounds showed less pronounced cytotoxities against three tested human cancer cell lines and no compounds displayed antibacterial activities toward four indicator strains.

  3. Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonas sp.

    Science.gov (United States)

    Sutili, Fernando J; Cunha, Mauro A; Ziech, Rosangela E; Krewer, Carina C; Zeppenfeld, Carla C; Heldwein, Clarissa G; Gressler, Leticia T; Heinzmann, Berta M; Vargas, Agueda C; Baldisserotto, Bernardo

    2015-03-01

    In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonas sp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1 for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%.

  4. PLASTICIDAD FENOTÍPICA DE Lippia alba Y Lippia origanoides (VERBENACEAE EN RESPUESTA A LA DISPONIBILIDAD DE LUZ Phenotypic Plasticity of Lippia alba and Lippia origanoides (Vervenaceae in Response to Availability of Light

    Directory of Open Access Journals (Sweden)

    EDWIN PARRA TORRES

    Full Text Available La distribución de la especies es usualmente relacionada con la magnitud de la plasticidad fenotípica (PF. Con el fin de examinar la posible relación entre la magnitud de la PF y la amplitud ecológica en respuesta a la disponibilidad de luz, este trabajo evaluó la PF a través de la ontogenia en clones de Lippia alba y Lippia origanoides, especies congenéricas que presentan diferencias en su distribución. Clones de cada especie fueron distribuidos aleatoriamente en tres tratamientos, baja (33%, media (53%, y alta (100% disponibilidad lumínica. Se evaluó la PF de caracteres morfológicos y de asignación de biomasa a través de la ontogenia por medio de un análisis de varianza (ANAVA y la comparación interespecífica se realizó a través del índice de plasticidad fenotípica (RDPI. L. alba y L. origanoides presentaron variación de la plasticidad a través de la ontogenia en varios de los caracteres estudiados. La comparación interespecífica a través del RDPI mostró que la mayor PF de L. alba sobre L. origanoides en algún estado de la ontogenia no fue consistente ni uniforme a través de esta. Estos resultados sugieren una débil asociación entre la magnitud de la PF en respuesta a la disponibilidad lumínica. Además, indican que la mayor distribución de L. alba, se debe a un mayor desempeño en la tasa de crecimiento relativa y consecuentemente a una mayor acumulación de biomasa total, que posiblemente, le permiten alcanzar rápidamente su madurez sexual y así la colonización de nuevas áreas.The distribution of the plant species is usually related to the magnitude of the phenotypic plasticity (PF. With the purpose of to stablish the possible relationship between the magnitude of the PF and the ecological breadth in response to light availability, the PF during the ontogeny in clones of Lippia alba and Lippia origanoides was evaluated. Both species are congeneris and show differences in their distribution. Three random

  5. Toxicity of eight polycyclic aromatic compounds to the red clover (Trifolium pratense), ryegrass (Lolium perenne), and mustard (Sinapsis alba)

    DEFF Research Database (Denmark)

    Sverdrup, L. E.; Krogh, P. H.; Nielsen, T.

    2003-01-01

    The effect of eight polycyclic aromatic compounds (PACs) on the seed emergence and early life-stage growth of three terrestrial plants (Sinapsis alba, Trifolium pratense and Lolium perenne) were studied in a greenhouse, using a Danish agricultural soil with an organic carbon content of 1.6%. After...

  6. Toxicity of eight polycyclic aromatic compounds to red clover (Trifolium pratense), ryegrass (Lolium perenne), and mustard (Sinapsis alba)

    DEFF Research Database (Denmark)

    Sverdrup, L.E.; Krogh, P.H.; Nielsen, T.

    2003-01-01

    The effect of eight polycyclic aromatic compounds (PACs) on the seed emergence and early life-stage growth of three terrestrial plants (Sinapsis alba, Trifolium pratense and Lolium perenne) were studied in a greenhouse, using a Danish agricultural soil with an organic carbon content of 1.6%. After...

  7. Sex-specific costs of resistance to the fungal pathogen Ustilago violacea (Microbotryum violaceum) in Silene alba

    NARCIS (Netherlands)

    Biere, A.; Antonovics, J.

    1996-01-01

    Costs of resistance are often invoked to explain the maintenance of polymorphisms for resistance to fungal pathogens in natural plant populations. To investigate such costs, 27 half-sib families of Silene alba, collected from a single host population, were grown in experiment populations in the pres

  8. Impact of flowering phenology of Silene alba and S-dioica on susceptibility to fungal infection and seed predation

    NARCIS (Netherlands)

    Biere, A.; Honders, S.C.

    1996-01-01

    Timing of the production of susceptible host stages can have a large impact on a plant's probability of infection by pathogens and on the extent of damage caused by herbivores. In the closely related plant species Silene alba and S. dioica, flowers are the site of infection by the host-sterilizing a

  9. EFFECTS OF LIGHT AND TEMPERATURE ON Tabebuia serratifolia (Vahl Nich,Tabebuia chrysotricha (Mart. ex DC. Standl. AND Tabebuia roseo-alba (Ridl Sand – Bignoniaceae SEED GERMINATION

    Directory of Open Access Journals (Sweden)

    Débora Leonardo dos Santos

    2010-08-01

    Full Text Available Tabebuia serratifolia, Tabebuia chrysotricha and Tabebuia roseo-alba (Bignoniaceae, are an important ornamental and wood trees in Brasil. The effects of light and temperature in the  germination of Tabebuia serratifolia, Tabebuia chrysotricha and Tabebuia roseo-alba seeds were studied in the present work. The results indicated that the species present light insensitive seeds the and optimum temperature was between 20° and 30°C, the maximum temperature between 35° and 40°C and minimal temperature between 10° and 15°C for Tabebuia chrysotricha and Tabebuia roseo-alba and below 10°C for Tabebuia serratifolia.

  10. Antidiabetic Effects of Aqueous Infusions of Artemisia herba-alba and Ajuga iva in Alloxan-Induced Diabetic Rats.

    Science.gov (United States)

    Boudjelal, Amel; Siracusa, Laura; Henchiri, Cherifa; Sarri, Madani; Abderrahim, Benkhaled; Baali, Faiza; Ruberto, Giuseppe

    2015-06-01

    The aqueous infusions of the aerial parts of Artemisia herba-alba Asso and Ajuga iva Schreber, prepared in accordance with the traditional procedure used in the local folk medicine, have been analysed for their composition and content of phytochemical constituents and examined for their antidiabetic effectiveness in alloxan-induced diabetic rats. Oral administration of A. herba-alba and A. iva infusions was studied in normal and alloxan-induced diabetic rats, which were randomly divided into nine groups, each group consisting of six animals. The drug preparations (100, 200, and 300 mg/kg b. w.) of each plant were given orally to the rats of each group twice daily for 15 days. Compositional analysis of the aqueous infusions revealed the presence of several polyphenols as main components. A. herba-alba infusion was characterised by mono- and di-cinnamoylquinic acids, with 5-caffeoylquinic (chlorogenic) acid being the main compound, followed by 3,5-dicaffeoylquinic acid. Vicenin-2 (apigenin 6,8-di-C-glucoside) appeared to be the most abundant among flavonoids. On the other hand, A. iva showed the exclusive presence of flavonoids, with the flavanone naringin present in relatively high levels together with several apigenin (flavone) derivatives. Oral administration of 300 mg/kg b. w. of the aqueous infusions of A. herba-alba and A. iva exhibited a significant reduction in blood glucose content, showing a much more efficient antidiabetic activity compared to glibenclamide, the oral hypoglycaemic agent used as a positive control in this study. These results suggest that A. herba-alba and A. iva possess significant antidiabetic activity, as they were able to improve the biochemical damage in alloxan-induced diabetes in rats. Georg Thieme Verlag KG Stuttgart · New York.

  11. Pre-sedation and transport of Rhamdia quelen in water containing essential oil of Lippia alba: metabolic and physiological responses.

    Science.gov (United States)

    Becker, Alexssandro G; Parodi, Thaylise V; Zeppenfeld, Carla C; Salbego, Joseânia; Cunha, Mauro A; Heldwein, Clarissa G; Loro, Vania L; Heinzmann, Berta M; Baldisserotto, Bernardo

    2016-02-01

    The effects of transporting silver catfish (Rhamdia quelen) for 6 h in plastic bags containing 0 (control), 30 or 40 µL/L of essential oil (EO) from Lippia alba leaves were investigated. Prior to transport, the fish in the two experimental groups were sedated with 200 µL/L of EO for 3 min. After transport, dissolved oxygen, carbon dioxide, alkalinity, water hardness, pH, temperature and un-ionized ammonia levels in the transport water did not differ significantly among the groups. However, total ammonia nitrogen levels and net Na(+), Cl(-) and K(+) effluxes were significantly lower in the groups transported with EO of L. alba than those in the control group. PvO2, PvCO2 and HCO3(-) were higher after transporting fish in 40 µL/L of EO of L. alba, but there were no significant differences between groups regarding blood pH or hematocrit. Cortisol levels were significantly higher in fish transported in 30 µL/L of EO of L. alba compared to those of the control group. The metabolic parameters (glycogen, lactate, total amino acid, total ammonia and total protein) showed different responses after adding EO to the transport water. In conclusion, while the EO of L. alba is recommended for fish transport in the conditions tested in the present study because it was effective in reducing waterborne total ammonia levels and net ion loss, the higher hepatic oxidative stress in this species with the same EO concentrations reported by a previous study led us to conclude that the 10-20 µL/L concentration range of EO and lack of pre-sedation before transport are more effective.

  12. Morteros de albañilería con escombros de demolición

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    Álvarez Cabrera, J. L.

    1997-06-01

    Full Text Available The possibility of recycling construction rubble, its technical and economic effectiveness, and the social and ecological impact they provide for our society is the reason and the starting point for an optimal exploitation of this aggregate in the manufacture of masonry mortar, since it has the same inherent properties as those obtained from the quarry aggregates, described in the Cuban standard NC52-79:93. The work consists in the preparation and study of masonry mortars using recycled aggregates -demolition product-. Four volumetric dosages were prepared (1:4; 1:5; 1:6 y 1:8, which were submitted to physical and mechanical tests at 7, 28 and 60 days, adherence resistance at 28 days, and capillary absorption, obtaining similar results to those.

    La posibilidad real del reciclaje de escombros, su efectividad técnica, económica, y el impacto social y ecológico que proporciona a la sociedad, es nuestro punto de partida para lograr un óptimo aprovechamiento de este árido como futuro agregado fundamental en la obtención de un mortero de albañilería, capaz de tener como propiedades aquellas intrínsecas de los propios morteros obtenidos a partir de áridos de cantera y recogidos en la norma cubana NC52-79:93. El trabajo consiste en la preparación y estudio de morteros de albañilería empleando árido reciclado -producto de demolición- como agregado. Se prepararon 4 dosificaciones volumétricas (1:4; 1:5; 1:6 y 1:8, las cuales fueron sometidas a ensayos físico-mecánicos al, 28 y 60 días, resistencia a la adherencia a 28 días, y absorción capilar, obteniéndose resultados comparables con los morteros de albañilería tradicionales.

  13. Toxicity Of The Essential Oil Of The Cytral Chemotype Of Lippia Alba (Mill. N. E. Brown Toxicidad del aceite esencial de Lippia alba (Mill. N. E. Brown quimiotipo citral

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    Jesús Olivero-Verbel

    2010-06-01

    Full Text Available The essential oil (EO of Lippia alba (Mill. N. E. Brown (Verbenaceae has been traditionally used to treat several diseases. In this study, the acute toxic effects of the citral chemotype of L. alba EO were evaluated in mice. Animals were treated via intraperitoneal receiving the L. alba essential oil at doses between 50 and 2500 mg/kg, and the control group received sesame oil (vehicle. The EO induced dose-dependent neurotoxic effects at doses greater than 1000 mg/kg, including decreased locomotion, motor skills and muscle strength, hypotonia, dyspnea, kyphosis and convulsions. The EO was lethal at a dose of 2500 mg/kg. Animals receiving 1000 mg/kg were euthanized at the end of the treatment period and their blood and livers were collected for analysis. Mice exposed to L. alba EO presented signifcantly greater plasma alanine aminotransferase (ALT activities than the control group. Liver histological changes included mild infammation, in particular, an increase in nuclear size. Compared to vehicle control group, changes in expression for selected genes were signifcant for FABP5, a fatty acid transport related gene. In summary, the intraperitoneal administration of L. alba EO (citral chemotype causes neurological damage in mice at doses equal or greater than 1500 mg/kg, whereas at 1000 mg/kg, it generates mild liver damage. Therefore, the systemic use of this EO raises concerns about its safety.El aceite esencial (AE de Lippia alba (Mill. NE Brown (Verbenaceae ha sido utilizado tradicionalmente para tratar varias enfermedades. En este estudio, los efectos tóxicos agudos del AE de Lippia alba quimiotipo citral fueron evaluados en ratones. Los animales fueron tratados por vía intraperitoneal recibiendo el AE en dosis entre 50 y 2500 mg/kg de peso, y el grupo control aceite de sésamo (vehículo. Dosis superiores a 1000 mg/kg del AE mostraron efectos neurotóxicos incluyendo disminución de la locomoción e hipotonía, disnea, cifosis y

  14. Características morfo-anatômicas e bromatológicas de folhas de amoreira em relação às preferências do bicho-da-seda Mulberry leaf morphological, anatomical and bromatological characteristics in relation to silkworm preferences

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    Fumiko Okamoto

    2006-02-01

    Full Text Available O objetivo deste trabalho foi avaliar, quantitativamente, as características morfo-anatômicas e bromatológicas das folhas de dez cultivares de amoreira, e estabelecer as relações de preferência do bicho-da-seda, por essas cultivares. Foram coletadas as folhas superior (5ª e mediana (15ª da planta, contadas a partir do ápice. As cultivares foram comparadas pelos métodos estatísticos multivariados de análise de agrupamento e análise de componentes principais. As cultivares Korin, Calabresa, IZ 5/2 e IZ 15/7 foram consideradas as mais recomendáveis para a alimentação das lagartas do bicho-da-seda, pois apresentaram valores altos de porcentagem de folha consumida, atribuídos à ocorrência de características desejáveis nas folhas, tais como menor teor de fibra bruta, menor quantidade de idioblastos de cistólito e de mucilagem, menor proporção de epiderme e maior de parênquima, comparativamente, às outras cultivares estudadas. As cultivares IZ 13/6 e IZ 57/2 foram consideradas as menos recomendáveis. As demais cultivares comportaram-se como intermediárias na preferência do bicho-da-seda.The objective of this work was to evaluate quantitatively the morphological, anatomical and bromatological characteristics of leaves of ten mulberry cultivars, and to establish the preferences shown by the silkworm. The upper (5th and median (15th leaves were collected taking branch top into account. In order to compare cultivars, multivariate statistic methods of cluster analysis and principal component analysis were used. The cultivars Korin, Calabresa, IZ 5/2 and IZ 15/7 were considered as the most appropriate to feed silkworms, since they presented high percentage values of eaten leaves, due to certain characteristics such as lower crude fiber contents, lower quantity of cystolith and mucilage idioblasts, lower proportions of epidermis and higher quantities of parenchyma, in relation to the other studied cultivars. Cultivars IZ 13/6 and

  15. Production capacity of biomass of the floodpain community of Salix alba L. in southern Moravia

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    Diana López

    2007-01-01

    Full Text Available The paper deals with the study of the production capacity of biomass in the seven-year stand of Salix alba L. The communities originated in the process of primary succession in the area of the middle Nové Mlýny reservoir on a newly established island. Already since the first stages, the communities have been monitored. Results have shown that white willow behaves as an R-strategist with fast growth in youth. Moreover, the growth is supported by optimum environmental conditions (soils richly supplied with nutrients and water, long growing season. Accumulated phytomass amounted 102,7 t.ha−1 at the age of 7 years and the yield reached a mean annual increment of 15 t.ha−1.year−1. Communities of white willow rank among highly productive phytocoenoses capable of fixing considerable amounts of carbon and, at the same time fulfilling the function of habitat corridors.

  16. Variability of physiological and growth characteristics of white willow (Salix alba L. clones

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    Orlović Saša

    2006-01-01

    Full Text Available The samples from field experiment on five clones of white willow (Salix alba L. in the adult phase, was investigated as follows: net photosynthesis and dark respiration, content of photosynthetic pigments (chlorophylls a, b and carotenoids, number and size of stornata and elements of growth (tree diameters and heights. The aim of the research is to determine if there are any relations between these physiological characters and the elements of growth, i.e. if any of them can be utilized in the early selection for growth vigor. The results show that all the characters are characterized by low coefficients of variation, statistically highly significant differences and high coefficients of heritability in a broad sense. As for the net of photosynthesis, the number of stornata on the adaxial and abaxial sides of the leaf are in high correlation with the elements of growth, which indicates that they can be used in the early selection for growth vigor.

  17. THERMO-VACUUM MODIFICATION OF SPRUCE (PICEA ABIES KARST. AND FIR (ABIES ALBA MILL. WOOD

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    Ottaviano Allegretti,

    2012-06-01

    Full Text Available The study presents results of the characterization of Norway spruce (Picea abies Karst. and fir (Abies alba Mill. wood thermally modified by TERMOVUOTO® technology at temperatures in the range of 160 to 220°C in vacuum conditions. Sixteen thermo-vacuum treatment tests were carried out using a pilot laboratory unit on 30-mm-thick spruce and fir boards in various combinations of the process parameters, i.e. temperature (T, duration (t, and pressure (p. The treated material was characterized to reveal the changes of the physical-mechanical properties including color and durability. The treated wood showed an improved performance with relation to the dimensional stability and durability. The measured mechanical properties did not show any significant decrease. Analytical models, based on the existing correlations between wood properties and process parameters, were assessed, thus allowing the control of the process.

  18. ASPECTS CONCERNING TAX EVASION IN ALBA COUNTY AND BRAŞOV REGION

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    Claudia Florina Radu

    2014-09-01

    Full Text Available One of the effects of evasion is the reduction in tax revenues, so the state will have less money to supply public goods and services. Instead, if there are sustained efforts to reduce tax evasion, revenues to the budget will increase, will exist resources to counter the economic crisis and for providing public services to citizens: education, health, social security etc. Additional money obtained from reducing tax evasion would solve many of the problems related to the lack of resources facing states today. That is why it is so important to combat and reduce tax evasion, both nationally and globally. Starting from the analysis of some data about tax audit and budget revenues of Alba County, we highlighted the main factors that determine people to pay taxes: firstly, the positive incentives, then the fear of negative consequences of non-payment, and, to a small extent, the moral factors.

  19. Impact du stress hydrique sur le fonctionnement hydraulique foliaire du peuplier Populus tremula x alba

    OpenAIRE

    Daaboul, Philippe

    2015-01-01

    Afin d’aborder l’impact du stress hydrique sur le fonctionnement hydraulique foliaire, des plants de Populus tremula x alba ont été soumis à un stress hydrique modéré ou sévère pendant une semaine par ajout de PEG dans la solution nutritive. La mesure de paramètres écophysiologiques et moléculaires tissus spécifiques a permis de dégager plusieurs tendances sur la caractérisation de l’influence du stress. Les deux types de stress n’ont que peu d’impact sur la croissance et la capacité de synth...

  20. The use of the white poplar (Populus alba L.) biomass as fuel

    Institute of Scientific and Technical Information of China (English)

    Tatiana Griu; Aurel Lunguleasa

    2016-01-01

    We determined the calorific value of white poplar (Populus alba L.) woody biomass to use it as fire-wood. The value of 19.133 MJ kg-1 obtained experimen-tally shows that the white poplar can be quite successfully used as firewood. Being of a lower quality in comparison with usual beech firewood, the white poplar has similar calorific value. The white poplar has a calorific density of 30.7%lower than that of current firewood. That is why the price of this firewood from white poplar is lower accord-ingly. Also, the prognosis of calorific value on the basis of the main chemical elements, being very close to the experimental value (?2.6%), indicates an appropriate value can be achieved to be used for investigation with the chemical element analysis.

  1. Essential oil of Lippia alba in the transport of Nile tilapia

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    Janis Cumming Hohlenwerger

    Full Text Available ABSTRACT: This study aimed to examine the action of the essential oil of Lippia alba (EOLA in the stress response for transport of Nile tilapia Oreochromis niloticus . The fish were transported into three treatments (in triplicate: control, 10 and 20 µL L-1 EOLA, with loading density of 15 fish/plastic bags for 8h. Plasma glucose levels were significantly decreased in fish exposed to 20µL L-1 EOLA in comparison with the control group and fish exposed to 10µL L-1 EOLA, but the plasma cortisol, lactate and paraoxonase levels were similar. Un-ionized ammonia and ventilatory rate demonstrated a significant reduction in the treatments with the use of EOLA. In conclusion the use of 20µL L-1 EOLA is indicated for Nile tilapia transport.

  2. Screening of Antimicrobial activity of Aqueous extracts of Leaves, Flower and Stem of Eclipta alba

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    Prabhsimran Singh Sandhu

    2012-12-01

    Full Text Available Plants are the oldest source of pharmacologically active compounds, and have provided humankind with many medically useful compounds for centuries. In this study aqueous extract of leaves, stem and flower of Eclipta alba were tested for antimicrobial activity against Escherichia coli (ATCC25923,Enterobacter cloacae (ATCC10699, Enterococcus faecalis (ATCC10741, Proteus vulgaris (ATCC12454 Staphylococcus aureus (ATCC25923 Klebsiella pneumonia (ATCC15380 and Staphylococcus. Saprophyticus (ATCC35552 It was shown that leaves extract effective against E. cloacae & K .pneumoniae but not against others, while aquous extract of stem shown good antitimicrobial effect against E. cloacae, E. faecali, K. pneumoniae and S. saprophyticus but E. coli, P. vulgaris, S. aureus were found resistant and The aqueous extract of flower shown reliabe ZOI against P.vulgaris ,S.aureus and S.saprophyticus while resistant against all other microbes.

  3. Bio-assay guided isolation of alpha-glucosidase inhibitory constituents from Eclipta alba.

    Science.gov (United States)

    Kumar, Deepak; Gaonkar, Raghuvir H; Ghosh, Rina; Pal, Bikas C

    2012-08-01

    Eclipta alba (L.) Hassk is used traditionally in diabetes mellitus in India and the plant extract is reported to possess anti-diabetic activity. A bioactivity-guided isolation approach based on alpha-glucosidase inhibition was used to identify the constituents contributing towards the inhibition of the enzyme and probably contributing towards its anti-diabetic activity. Four echinocystic acid glycosides were thus isolated, of which eclalbasaponin VI, isolated from the n-butanol fraction, was found to be the most potent (IC50 54.2 +/- 1.3 microM). The compound is an uncompetitive type of inhibitor with Ki 26.1 microM. A quantitative estimation of the constituents was established using RP-HPLC.

  4. Mycocoenology in Abies alba Miller woods of central-southern Tuscany (Italy

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    Angela Laganà

    2014-01-01

    Full Text Available Numerous reports indicate that fir woods in central and northern Europe have recently been damaged by increasing pollution. It has been demonstrated that fungi can be good bioindicators of forest health status. In polluted areas the production of fruit bodies generally declines and the fungal biodiversity, especially of symbiotic species, is reduced. Here we report the results of a survey of the fungal and plant communities in woods of Abies alba Miller in central-southern Tuscany, already studied in the past. Certain changes were observed in the study areas, but they seem more likely to be due to other factors than pollution, such as the weather conditions, the age and natural evolution of the forests towards their climax.

  5. Pedestrian Zones As Important Urban Strategies in Redeveloping the Community - Case Study: Alba Iulia Borough Park

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    Oana Elena BLAGA

    2013-02-01

    Full Text Available The pedestrian zone issue is by far an important matter in the context of urban regeneration. Cities which adopted this strategy – the pedestrian zones – have recorded better urban attitudes regarding the urban environment, a continuous growth of the urban quality, an improved urban ecosystem  and continuous attractiveness for investment and  tourism. This article explores the evolution of the pedestrian zones as ideas in utopian urban models in the early 1900 and later as efficient environmental friendly strategies adopted by cities. After identifying the path this concept followed, from a simple idea to an important strategy of urban development, the paper focuses on the major characteristics and benefits of the pedestrian precincts. Next, the article focuses on the newest pedestrian zone in one of the Romanian cities, Alba Iulia and it tries to identify the types of impact this area has so far on the community and entire city.

  6. Concentrações de BAP sobre a proliferação in vitro de brotos de Lippia alba [(Mill.N.E.Brown] BAP concentrations on in vitro proliferation of Lippia alba [(Mill. N.E.Brown] shoots

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    S.A. Asmar

    2012-01-01

    Full Text Available Lippia alba é uma planta de origem brasileira, pertencente à família Verbenaceae. É conhecida por combater a insônia e a asma, além de possuir ação anticonvulsivante e antifúngica. A micropropagação tem sido utilizada para multiplicação de várias espécies com propriedades medicinais, e o BAP é a citocinina sintética mais utilizada para a multiplicação in vitro. O objetivo deste trabalho foi estabelecer a concentração adequada de BAP na multiplicação in vitro de L. alba. Explantes constituídos de segmentos nodais provenientes de plântulas já estabelecidas in vitro com aproximadamente 0,5 cm foram inoculados em meio MS, suplementado com 0,0; 0,5; 1,0 e 1,5 mg L-1 de BAP em adição de 30 g L-1 de sacarose. A utilização de 1,5 mg L-1 de BAP promove a multiplicação in vitro de L. alba. A ausência deste regulador propicia maior número de folhas e o uso na concentração de 0,5 mg L-1 aumentou as massas fresca e seca de parte aérea nesta espécie.Lippia alba is a Brazilian plant, belonging to the Verbenaceae family. It is known to combat insomnia and asthma, also has antifungal and anticonvulsant actions. Micropropagation has been used for propagation of various species with medicinal properties, and BAP is the most widely used synthetic cytokinin for in vitro multiplication. The aim of this study was to determine the appropriate concentration of BAP on in vitro multiplication of L. alba. Explants consisting of nodal segments from seedlings already established in vitro with approximately 0.5 cm were inoculated on MS medium supplemented with 0; 0.5; 1.0 and 1.5 mg L-1 of BAP and 30 g L-1 of sucrose. The use of 1.5 mg L-1 of BAP promotes the in vitro multiplication of L. alba. The absence of this regulator provides a greater number of leaves and its use at the concentration of 0.5 mg L-1 increased shoots' fresh and dry weights in this specie.

  7. Lippia alba morphotypes cidreira and melissa exhibit significant differences in leaf characteristics and essential oil profile

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    Caroline N. Jezler

    2013-04-01

    Full Text Available Lippia alba (Mill. N.E. Br. ex Britton & P. Wilson, Verbenaceae, is widely used in traditional Brazilian medicine for the treatment of abdominal distress. The species exhibits considerable chemical and morphological diversity, and various chemotypes have been characterized. A comparative study of L. alba, has been carried out of the morphoanatomical characteristics of the leaves and the profiles of the essential oils of the morphotypes cidreira and melissa grown in the Medicinal Plant Garden of the Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil. The mean plant height of cidreira was 1.80 m and the stems and branches were fairly erect, while melissa plants were smaller (1.60 m and presented prostrate stems and branches. Although the leaf of the morphotypes look were similar, the mean values of length, width and area of the leaves of cidreira (respectively, 7.42 cm, 3.32 cm and 17.31 cm² differed significantly from those of melissa (4.68 cm, 2.35 cm and 7.32 cm2. The morphotypes presented amphistomatic leaves with uniseriate epidermis on both surfaces. The mesophyll was dorsiventral, but in cidreira the palisade parenchyma was biseriate while in melissa it was uniseriate. Simple tector and capitate glandular trichomes were present on the adaxial and abaxial surfaces of the leaf blades of both morphotypes. Six distinct types of glandular trichomes could be distinguished: types I and II were present in both morphotypes, while type III was detected only in cidreira, and types IV to VI were present only in melissa. The two morphotypes also differed with respect to the composition of the essential oil, cidreira produced oil composed mainly of citral, while the oil from melissa was rich in citral, limonene and carvone.

  8. Maurits Ebben, Margriet Lacy-Bruijn, and Rolof van Hövell tot Westerflier (eds., Alba: General and Servant to the Crown

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    José Eloy Hortal Muñoz

    2015-12-01

    Full Text Available Maurits Ebben, Margriet Lacy-Bruijn, and Rolof van Hövell tot Westerflier (eds., Alba:  General and Servant to the Crown (Rotterdam: Karwansaray publishers, 2013, 464 pp., isbn 978 94 90258 08 5.

  9. Optimization of Extraction Conditions and Development of a Sensitive HPTLC Method for Estimation of Wedelolactone in different extracts of Eclipta alba

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    Kulkarni Savita

    2011-01-01

    Full Text Available Eclipta alba has been used in Ayurvedic medicine for treatment of various ailments. An attempt has been made to optimize different extraction conditions for Eclipta alba. Further an accurate, precise, reproducible and sensitive accurate, precise and reproducible (HPTLC method has been developed for the estimation of Wedelolactone in Eclipta alba. An attempt has been made to quantify wedelolactone in various extracts by HPTLC method. Precoated aluminum silica gel F plates were used as stationary phase and toluene: ethyl acetate (9:1 was used as mobile phase. This system was found to give compact spots for wedelolactone (RF value of 0.30 ± 0. 3 with a linearity range of 1 to 80 µg per spot. The proposed method was applied to evaluate efficiency of different methods of extraction i.e. percolation, maceration, hot solvent extraction (Soxhlet apparatus, supercritical fluid extraction, microwave, orbital shaker bath and sonication method for extraction of wedelolactone from Eclipta alba.

  10. Reducing economic disparities between rural and urban areas through integrated development – Case study: analysis of the turnover of companies in the AIDA Association, Alba County, Romania

    OpenAIRE

    Ionela Gavrilă-Paven; Emil Olteanu; Iulian Bogdan Dobra

    2014-01-01

    This paper presents a local initiative in Romania, Alba County, which tries to establish general guidelines for the sustainable development of a local community that consists of three towns and eight villages. The analysis conducted in this paper is focused on economic activity. The turnover of the companies located in the eleven localities that compose the Intercommunity Development Association – Alba Iulia have been studied. The authors’ objective is to determine the influenc...

  11. Fortification of dark chocolate with spray dried black mulberry (Morus nigra) waste extract encapsulated in chitosan-coated liposomes and bioaccessability studies.

    Science.gov (United States)

    Gültekin-Özgüven, Mine; Karadağ, Ayşe; Duman, Şeyma; Özkal, Burak; Özçelik, Beraat

    2016-06-15

    Fine-disperse anionic liposomes containing black mulberry (Morus nigra) extract (BME) were prepared by high pressure homogenization at 25,000 psi. Primary liposomes were coated with cationic chitosan (0.4, w/v%) using the layer-by-layer depositing method and mixed with maltodextrin (MD) (20, w/v%) prior to spray drying. After that, spray dried liposomal powders containing BME were added to chocolates with alkalization degrees (pH 4.5, 6, 7.5) at conching temperatures of 40 °C, 60 °C, and 80 °C. The results showed that, compared to spray dried extract, chitosan coated liposomal powders provided better protection of anthocyanin content in both increased temperature and pH. In addition, encapsulation in liposomes enhanced in vitro bioaccessability of anthocyanins. Chocolate was fortified with encapsulated anthocyanins maximum 76.8% depending on conching temperature and pH. Copyright © 2016. Published by Elsevier Ltd.

  12. Proyecto Grannacional ALBASALUD para Centro Regulador de Medicamentos Esenciales de los países del ALBA-TCP Grand-national Project ALBASALUD for Regulatory Center of Essential Drugs for the ALBA-TCP countries

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    Celeste Aurora Sánchez González

    2011-09-01

    Full Text Available La Autoridad Reguladora de Medicamentos de Cuba, coordina un Proyecto de la Alianza Bolivariana para los pueblos de América-Tratado de Libre Comercio (ALBA-TCP, dirigido al desarrollo de un Centro Regulador para los países del ALBA como nuevo organismo regional de integración farmacéutica, en aras de contar con un Registro Grannacional válido en todos los países partes, como herramienta para facilitar el acceso a medicamentos esenciales de calidad. Esta investigación tuvo como objetivo diseñar y conducir desde el punto de vista técnico un proyecto para crear las bases legales y metodológicas del futuro centro y sus funciones básicas. Se emplearon técnicas de grupo nominal, puntos de referencia, consultas a expertos y se crearon metodologías específicas para planear y confeccionar los documentos necesarios. Se utilizaron lineamientos de organismos reguladores internacionales, cuadros básicos de medicamentos y reglamentación farmacéutica de los países participantes. Se desarrollaron y aplicaron documentos y estrategias para el trabajo del proyecto durante la etapa de preinversión, se definió el primer listado de medicamentos esenciales del ALBA y se aprobaron las fundamentales disposiciones legales y la reglamentación para las funciones de registro, vigilancia, inspecciones, laboratorios y liberación de lotes. El Proyecto Grannacional ALBASALUD "Centro Regulador de Medicamentos del ALBA-TCP" aplicado ha demostrado su eficiencia y capacidad para alcanzar los objetivos trazados mediante un trabajo científico-técnico con participación colectiva, que ha garantizado las disposiciones y lineamientos requeridos para sus funciones básicas y respaldo legal.The Drug Regulatory Authority of Cuba is coordinating a Project of the Bolivarian Alliance for the Peoples of America- Free Trade Agreement (ALBA-TCP aimed at developing a Regulatory Center that will operate as a new regional body of pharmaceutical integration for the ALBA

  13. Effect of black mulberry (Morus nigra) extract treatment on cognitive impairment and oxidative stress status of D-galactose-induced aging mice.

    Science.gov (United States)

    Turgut, Nergiz Hacer; Mert, Derya Guliz; Kara, Haki; Egilmez, Hatice Reyhan; Arslanbas, Emre; Tepe, Bektas; Gungor, Huseyin; Yilmaz, Nese; Tuncel, Necati Baris

    2016-01-01

    Morus nigra L. (Moraceae) has various uses in traditional medicine. However, the effect of M. nigra on cognitive impairment has not been investigated yet. The objective of this study is to determine the phenolic acid content and DNA damage protection potential of M. nigra leaf extract and to investigate the extract effect on cognitive impairment and oxidative stress in aging mice. Phenolic acid content was determined by quantitative chromatographic analysis. DNA damage protection potential was evaluated on pBR322 plasmid DNA. Thirty-two Balb-C mice were randomly divided into four groups (control, d-galactose, d-galactose + M. nigra 50, and d-galactose + M. nigra 100). Mice were administered d-galactose (100 mg/kg, subcutaneous) and M. nigra (50 or 100 mg/kg, orally) daily for 8 weeks. Behavioral responses were evaluated with Morris water maze. Activities of antioxidant enzymes and levels of malondialdehyde (MDA) were assayed in serum, brain, and liver. In extract, vanillic (632.093 μg/g) and chlorogenic acids (555.0 μg/g) were determined. The extract between 0.02 and 0.05 mg/mL effectively protected all DNA bands against the hazardous effect of UV and H2O2. Morus nigra significantly improved learning dysfunctions (p nigra has the potential in improving cognitive deficits in mice and that M. nigra may be useful to suppress aging, partially due to its scavenging activity of free radicals and high antioxidant capacity.

  14. Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10.

    Science.gov (United States)

    Gandhimathi, R; Seghal Kiran, G; Hema, T A; Selvin, Joseph; Rajeetha Raviji, T; Shanmughapriya, S

    2009-10-01

    A sponge-associated marine actinomycetes Nocardiopsis alba MSA10 was screened and evaluated for the production of biosurfactant. Biosurfactant production was confirmed by conventional screening methods including hemolytic activity, drop collapsing test, oil displacement method, lipase production and emulsification index. The active compound was extracted with three solvents including ethyl acetate, diethyl ether and dichloromethane. The diethyl ether extract was fractionated by TLC and semi-preparative HPLC to isolate the pure compound. In TLC, a single discrete spot was obtained with the R (f) 0.60 and it was extrapolated as valine. Based on the chemical characterization, the active compound was partially confirmed as lipopeptide. The optimum production was attained at pH 7, temperature 30 degrees C, and 1% salinity with glucose and peptone supplementation as carbon and nitrogen sources, respectively. Considering the biosurfactant production potential of N. alba, the strain could be developed for large-scale production of lipopeptide biosurfactant.

  15. Apuntes para una historia del gremio de albañiles de Quito. Ciudad y cultura popular

    Directory of Open Access Journals (Sweden)

    Eduardo Kingman

    2006-11-01

    Full Text Available A partir de las entrevistas realizadas a miembros del gremios de albañiles de Quito, el artículo formula algunas hipótesis sobre la formación de la cultura popular en la primera mitad del siglo XX. El gremio de albañiles se desarrolló en el contexto de una ciudad que pretendía ser moderna pero que, al mismo tiempo, reprodujo el privilegio y determinadas formas de violencia simbólica propias de una sociedad más tradicional. Frente a estas concepciones de privilegio, el estudio explora las nociones de raza popular y de respeto que emergen desde el espacio popular. Paralelamente, el artículo discute algunos problemas metodológicos y éticos relacionados con el método hagiográfico y con la posibilidad de escribir una historia de los trabajadores.

  16. Macromycetes of the Potentillo albae-Quercetum in the Świętokrzyskie Mts. - monitoring studies

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    Janusz Łuszczyński

    2014-08-01

    Full Text Available The paper contains analysis of macromycetes collected on two observation plots in the forest community Potentillo albae-Quercetum in the Świętokrzyskie Mountains. The aim of these investigation was to determine the participation and the role of macromycetes in the forest community with oak trees. During the investigation 182 taxons of macrofungi were collected, 33 species of them were connected with oak trees.

  17. Geomorphic signatures of glacial activity in the Alba Patera volcanic province: Implications for recent frost accumulation on Mars

    Science.gov (United States)

    Sinha, Rishitosh K.; Murty, Sripada V. S.

    2013-08-01

    landforms lying within impact craters on Mars have led to the identification of two mechanisms for their formation: (1) intermittent deposition of atmospherically emplaced snow/ice during past spin-axis/orbital conditions and (2) flow of debris-covered ice-rich deposits. The maximum presence of the young ice/snow-rich features (thermal contraction crack polygons, gullies, arcuate ridges, and lobate debris tongues) was observed on the pole-facing slope, indicating that this slope was the preferred site for ice/snow accumulation (during the last 10 Ma). In this study, we investigated 30 craters lying in the Alba Patera volcanic province in the latitudinal bands between 45°N and 32.4°N. Morphological comparison of the younger ice/snow-rich features in these craters led us to conclude that glacial/periglacial features in Alba Patera are mainly present within pole-facing slopes of craters lying within 45°N-39°N. The craters lying within 40.2°N-40°N did not show any glacial/periglacial features. We suggest that the formation of these young ice/snow-rich features follows the same orientation trends as those of other older (>10 Ma) glacial features (debris-covered ice/snow-rich large deposits at the base of the crater wall) in the region. The present work has revealed that the onset of physical processes that result in the formation of glacial/periglacial landforms is also dependent on the changes in elevation ranges of the investigated craters in Alba Patera. Our results confirm past inferences for accumulation of ice/snow on Mars and suggest that the period of ice/snow accumulation activity in Alba Patera occurred throughout the Amazonian and lasted until the recent past, i.e., 2.1-0.4 Ma.

  18. Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells.

    Science.gov (United States)

    Tofiño-Rivera, A; Ortega-Cuadros, M; Galvis-Pareja, D; Jiménez-Rios, H; Merini, L J; Martínez-Pabón, M C

    2016-12-24

    Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01mg/dL concentrations and of 93.1% in the 0.001mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24h. The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries. Copyright © 2016 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

  19. Evaluation of the effect of an organic extract obtained from Ipomoea alba L. on experimental periodontitis in rats

    OpenAIRE

    Guilherme Emerson Barrella; Ivana Barbosa Suffredini; Fernanda Vieira Ribeiro; Fabiano Ribeiro Cirano; Suzana Peres Pimentel

    2012-01-01

    The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493), on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group - vehicle-treated (n ...

  20. Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and bull Leydig cells

    Institute of Scientific and Technical Information of China (English)

    Paul F. Moundipa; Nathalie Sara E. Beboy; Fabien Zelefack; Silvère Ngouela; Etienne Tsamo; Wolf-Bernhard Schill; Thomas K. Monsees

    2005-01-01

    Aim: To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull,and to develop a novel in vitro test system using Leydig cells from bull testes. Methods: The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using 125I-radioimmunoassay (125I-RIA). Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes. Results: Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin (hCG). Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations. Conclusion: Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.

  1. Basella alba rubra spinach pigment-sensitized TiO2 thin film-based solar cells

    Science.gov (United States)

    Gokilamani, N.; Muthukumarasamy, N.; Thambidurai, M.; Ranjitha, A.; Velauthapillai, Dhayalan

    2015-03-01

    Nanocrystalline TiO2 thin films have been prepared by sol-gel dip coating method. The X-ray diffraction results showed that TiO2 thin films annealed at 400, 450 and 500 °C are of anatase phase and the peak corresponding to the (101) plane is present in all the samples. The grain size of TiO2 thin films was found to increase with increasing annealing temperature. The grain size is found to be 20, 25 and 33 nm for the films annealed at 400, 450 and 500 °C. The structure of the TiO2 nanocrystalline thin films have been examined by high-resolution transmission electron microscope, Raman spectroscopy and FTIR spectroscopy. TiO2 thin films were sensitized by natural dyes extracted from basella alba rubra spinach. It was found that the absorption peak of basella alba rubra extract is at about 665 nm. The dye-sensitized TiO2-based solar cell sensitized using basella alba rubra exhibited a J sc of 4.35 mA cm-2, V oc of 0.48 V, FF of 0.35 and efficiency of 0.70 %. Natural dyes as sensitizers for dye-sensitized solar cells are promising because of their environmental friendliness, low-cost production and fully biodegradable.

  2. Effects of auricular acupressure using Sinapsis alba seeds on obesity and self-efficacy in female college students.

    Science.gov (United States)

    Kim, Dongwon; Ham, Ok Kyung; Kang, Changwan; Jun, Eunmi

    2014-04-01

    To examine the effects of auricular acupressure with Sinapsis alba seeds on obesity and self-efficacy. Randomized controlled trial. College settings located in metropolitan areas of Korea. A total of 49 female college students who were overweight or obese (body-mass index [BMI] ≥25.0 kg/m(2)) were recruited and randomly assigned to the experimental group (n=25) or the control group (n=24). The experimental group applied three S. alba seeds to each of five auricular points (Shenmen, mouth, stomach, endocrine, and small intestine points). These participants were asked to stimulate those points 10 times at a rate of two times per second 30 minutes before mealtime, three times daily, for 1 month. They performed the procedure for each earlobe for alternating weeks (a total of 2 weeks' treatment for each ear). The obesity index included weight (kg), BMI (kg/m(2)), percentage body fat, and waist-to-hip ratio. Self-efficacy was measured by using a self-efficacy scale. Female students in the experimental group showed significant decreases in weight (t=10.76; p0.05) and waist-to-hip ratio (t=0.60; p>0.05) changes did not significantly differ between the two groups. These findings suggest that auricular acupressure using S. alba seeds may be an effective intervention for decreasing weight and BMI and increasing self-efficacy of overweight and obese individuals.

  3. Antimutagenic evaluation of traditional medicinal plants from South America Peumus boldus and Cryptocarya alba using Drosophila melanogaster.

    Science.gov (United States)

    Carmona, Erico R; Reyes-Díaz, Marjorie; Parodi, Jorge; Inostroza-Blancheteau, Claudio

    2017-01-01

    Peumus boldus Mol. ("Boldo") and Cryptocarya alba Mol. Looser ("Peumo") are medicinal shrubs with wide geographical distribution in South America. Their leaves and fruits are commonly used in traditional medicine because they exhibit natural medicinal properties for treatment of liver disorders and rheumatism. However, there are no apparent data regarding potential protective effects on cellular genetic components. In order to examine potential mutagenic and/or antimutagenic effects of these medicinal plants, the Drosophila melanogaster (D. melanogaster) wing-spot test was employed. This assay detects a wide range of mutational events, including point mutations, deletions, certain types of chromosomal aberrations (nondisjunction), and mitotic recombination. Qualitative and quantitative analyses of phenolic and anthocyanin compounds were carried out using biochemical and high-performance liquid chromatography methodologies. In addition, the antioxidant capacity of P. boldus and C. alba leaf extracts was also analyzed. P. boldus and C. alba extracts did not induce significant mutagenic effects in the D. melanogaster model. However, simultaneous treatment of extracts concurrently with the mutagen ethyl methane sulphonate showed a decrease of mutant spots in somatic cells of D. melanogaster, indicating desmutagenic effects in this in vivo model. Flavonoids and anthocyanins were detected predominantly in the extracts, and these compounds exerted significant antioxidant capacity. The observed antimutagenic effects may be related to the presence of phytochemicals with high antioxidant capacity, such as flavonoids and antohocyanins, in the extracts.

  4. Assessment Of Biologically Synthesized Ag Nanoparticles Toxicity Against E. coli, Staphylococcus aureus, Parachlorella kessleri And Sinapis alba

    Directory of Open Access Journals (Sweden)

    Kaduková Jana

    2015-06-01

    Full Text Available In general, Ag+ ions and AgNPs are considered to be the most toxic for bacterial cells and less toxic for higher organisms. In the present work inhibitory effects of biologically prepared silver nanoparticles on the growth of bacteria E. coli CCM 3954 and Staphylococcus aureus CCM 3953, green microscopic alga Parachlorella kessleri LARG/1 and seed germination and root growth of plant Sinapis alba seeds were investigated. Surprisingly, silver nanoparticles showed much stronger inhibitory effects on plant seed germination and root growth than on the bacterial growth. At concentration of 75 mg/l AgNPs both seed germination and root growth of Sinapis alba was inhibited whereas inhibition of the growth of E. coli and S. aureus was observed at >195 mg/l. Growth inhibition of alga Parachlorella kessleri was recorded at 300 mg/l AgNPs concentration. The inhibitory effect of silver ions was much higher compared to silver nanoparticles. Even 20 mg/l concentration of Ag+ ions inhibited the root growth and concentration > 45 mg/l inhibited germination of Sinapis alba seeds. Inhibition zones in both studied bacteria were found at concentration > 140 mg/l.

  5. Assessment of the repellent effect of Lippia alba essential oil and major monoterpenes on the cattle tick Rhipicephalus microplus.

    Science.gov (United States)

    Lima, A da Silva; Carvalho, J F de; Peixoto, M G; Blank, A F; Borges, L M F; Costa Junior, L M

    2016-03-01

    The control of Rhipicephalus microplus (Ixodida: Ixodidae) is achieved using synthetic acaricides. However, resistant tick populations are widespread around the world. Plant essential oils can act as repellents, keeping ticks away from hosts and decreasing the selection pressure on synthetic acaricides. The aim of this study was to evaluate the in vitro repellent effect of Lippia alba essential oil on R. microplus larvae. Leaves from two L. alba genotypes maintained under the same agronomic and environmental conditions were collected. Essential oil was extracted by hydrodistillation and analysed by gas chromatography-mass spectrometry (GC-MS). The major monoterpenes detected in the chemical analysis were commercially acquired and tested. For the repellency test, a glass rod was vertically fixed to measure active climbing of approximately 30 R. microplus larvae aged 14-21 days in response to essential oils and monoterpenes. Repellency was evaluated at 1 h, 3 h and 5 h after treatment. Variation in repellent action was detected between the genotypes. The major monoterpenes identified in the essential oils (limonene and carvone) showed low repellent effects in comparison with intact essential oils. Thus, the present results showed that L. alba essential oil contains bioactive compounds with great repellent activity against ticks that varies according to the plant genotype.

  6. Transportation of silver catfish, Rhamdia quelen, in water with eugenol and the essential oil of Lippia alba.

    Science.gov (United States)

    Becker, Alexssandro G; Parodi, Thaylise V; Heldwein, Clarissa G; Zeppenfeld, Carla C; Heinzmann, Berta M; Baldisserotto, Bernardo

    2012-06-01

    This study investigated the effectiveness of eugenol and of the essential oil (EO) of Lippia alba when used in the transport of the silver catfish (Rhamdia quelen). These investigations involved measurements of blood (pH, PvO(2), PvCO(2) and HCO(3) (-)) and water parameters, survival and ionoregulatory balance. Fish (301.24 ± 21.40 g, 28.90 ± 1.30 cm) were transported at a loading density of 169.2 g L(-1) for 4 h in fifteen plastic bags (7 L) divided into five treatments: control, 1.5 or 3.0 μL L(-1) of eugenol and 10 or 20 μL L(-1) of EO of L. alba. The water parameters were measured before (0 h) and after (4 h) transportation. The net Na(+), Cl(-) and K(+) losses were higher in fish from the control treatment compared to the other treatments. The PvO(2), PvCO(2) and HCO(3) (-) increased significantly in all of the treatments at the end of the transport period. In conclusion, based on the water (total ammonia nitrogen) and ionoregulatory indicators determined in the present study, our findings indicate that eugenol and the EO of L. alba are recommended for use in the transport of this species because these anesthetics apparently reduce stress.

  7. Utilización de la morera Morus indica L. como reemplazo parcial del concentrado en la crianza de terneras

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    Uribe Fernando

    1995-06-01

    Full Text Available

    Was made a technical and economical evaluation of three substitution levels of commercial concentrate (16% of crude protein by fresh Mulberry Morus indica Leaves, in growing replacement calves. Thirty calves of the Lucerna breed, weighing an average of 30 kg were fed during 112 days by means of the restricted suckling system. The statical analysis was a complete random desing, with three treatments: 100 (T1 75 (T2 and 50% concentrate (T3 two replicates were taken for each treatment and five calves were the experimental unit. The nutritional quality of Mulberry leaves was high, with 85.6% of dry matter degradability at 48 hours, 15.2% crude protein, 52 tonnes/ha/year with tour harvests per year and a cost of $137. I/kg of dry matter. The average weight gains were 0.406, 0.437, 0.406 kg/day and the concentrate consumptions 0.372, 0.207 and 0.115 kg/day, for T1 T2, and T3 respectively, with statistical difference (P<.05 for concentrate consumption. The Mulberry leaves consumption was 0.307 and 0.299 kg of D.M./day for T2  and T3, respectively. The economical analysis done by the partial budget technique indicated a net income of $190.864/calf for T2 followed for T3 with $185.027/calf and the for TI $178.109/calf.

    Se hizo una evaluación técnica y económica de tres niveles (T1:0, T2:25, y T3:50% de sustitución de concentrado comercial (16% de PC por hojas frescas de morera Monis indica en la cría de terneras de reemplazo. Se utilizaron 30 terneras de la raza Lucerna de cinco días (de edad y peso promedio de 30 kg, durante 112 días alimentadas por el sistema de amamantamiento restringido. El análisis de los resultados se hizo mediante un diseño completamente al azar, con tres tratamientos y dos repeticiones por tratamiento; la unidad experimental la constituyeron cinco terneras. La calidad nutricional de las hojas de morera

  8. Las formas verbales en las biblias de Alba y Ferrara: ¿fidelidad al texto hebreo?

    Directory of Open Access Journals (Sweden)

    del Barco del Barco, Francisco Javier

    2004-12-01

    Full Text Available This article focuses on the study of narrative and prophetic texts in the Ferrara and Alba Bibles and on their comparison to the Hebrew original. The aim of such a study is to find the keys to their systems of translation within the medieval Jewish tradition of Biblical translations. One of the most remarkable aspects is the translation of the verbal forms from Biblical Hebrew to Spanish. What is interesting about this aspect is how the translators understand the verbal system of Biblical Hebrew and how they converted it into 15th and 16th century Castillan, as well as the existing differences in the translation of both bibles; this fact will be illustrated with abundant examples. The linguistic aspect is offered here in its sociological and cultural context, as factors that affect translation methods.

    Este artículo se centra en el estudio de textos narrativos y proféticos de las biblias de Alba y Ferrara y en su comparación con el original hebreo. El fin de tal estudio es hallar las claves de sus sistemas de traducción dentro de la tradición judía medieval de traducciones bíblicas. Uno de los aspectos más destacables es la traducción de las formas verbales del hebreo bíblico al español. El interés en este punto está en ver cómo los traductores entienden el sistema verbal del hebreo bíblico y lo vierten al castellano de los ss. XV y XVI, así como en las diferencias de traducción existentes entre ambas biblias, que será ilustrado con numerosos ejemplos. El aspecto lingüístico se presenta aquí en su contexto sociológico y cultural, como factores que influyen en los métodos de traducción.

  9. Genetic and environmental characterization of Abies alba Mill. populations at its western rear edge

    Directory of Open Access Journals (Sweden)

    Sancho-Knapik, D.

    2014-12-01

    Full Text Available The genetic structure of ten Abies alba populations at the western rear edge in the Spanish Pyrenees was characterized and compared with two German populations, looking for the role of climatic factors in the fir decline. Growth, defoliation, aridity and cumulative summer water deficit summer were also characterized. Spanish populations show a lower genetic diversity and a high genetic differentiation than German ones, mainly established by an East (higher diversity-West (lower diversity gradient. The three defoliated populations are the western ones, with higher summer aridity. Contrastingly, the southern population (Guara shows climatic variables close to western sites but without defoliation and with a higher genetic diversity, indicating a possible adaptation to the sub-Mediterranean conditions. Silver fir in the Spanish Pyrenees constitutes a “stable” rear edge because of their isolation, small sized and small genetic diversity. Western Pyrenean sites subjected to dryer conditions and presenting lower genetic diversity are prone to drought-induced mortality in the context of global warming.Se ha caracterizado la estructura genética de diez poblaciones de Abies alba en la retaguardia occidental de su distribución en el Pirineo español en comparación con dos poblaciones de Alemania, buscando la influencia de factores climáticos en el decaimiento del abeto. También se caracterizó crecimiento, defoliación, aridez y déficit acumulado de agua durante el verano. Las poblaciones españolas mostraron una menor diversidad genética y una mayor diferenciación genética que las poblaciones alemanas, establecida principalmente a través de un eje Este (mayor diversidad-Oeste (menor diversidad. Las tres poblaciones con defoliación son las del suroeste, con mayor aridez estival. Por el contrario, la población más meridional (Guara muestra variables climáticas cercanas a estas poblaciones del suroeste pero sin defoliación y con mayor

  10. Wide Ranging Insect Infestation of the Pioneer Mangrove Sonneratia alba by Two Insect Species along the Kenyan Coast

    Science.gov (United States)

    Jenoh, Elisha Mrabu; Robert, Elisabeth M. R.; Lehmann, Ingo; Kioko, Esther; Bosire, Jared O.; Ngisiange, Noah; Dahdouh-Guebas, Farid; Koedam, Nico

    2016-01-01

    Insect infestation of mangroves currently threatens mangrove forest health and management. In the Western Indian Ocean region, little is known about insect damage to mangroves despite the fact that numerous infestations have occurred. In Kenya, infestations of Sonneratia alba have persisted for almost two decades, yet the taxonomic identity of the infesting pest(s), the extent of infestation, the pests’ biology, the impacts of infestation on host and the ecosystem, the host’s defensive strategies to the infestation are poorly understood. S. alba is a ubiquitous, pioneer mangrove species of the Indo-Pacific, occurring along the waterfront in a variety of mangrove ecosystem settings. Our main objectives were to identify the pest(s) responsible for the current dieback of S. alba in Kenya, and to determine the extent of infestation. To identify the pests responsible for infestation, we trapped emergent insects and reared larvae in the laboratory. To determine the overall extent of infestation within the S. alba zone, we assessed nine sites along the entire Kenyan coastline for the presence or absence of infested mangroves. Insect infestation in two mangrove embayments (Gazi and Mida) was quantified in depth. Two wood-boring insects were identified: a metarbelid moth (Lepidoptera, Cossoidea) of undescribed genus and the beetle Bottegia rubra (Cerambycidae, Lamiinae).The metarbelid moth infests mangroves in both northern (from Ngomeni to Kiunga) and southern regions (from Vanga to Mtwapa) of the Kenyan coast. B. rubra appeared in low density in Gazi, and in high density in Mida, Kilifi, and Ngomeni, with densities gradually decreasing northward. Insect infestation levels reached 18% in Gazi and 25% of S. alba stands in Mida. Our results indicate that B. rubra has the ability to infest young mangrove trees and expand its range, posing a danger to rehabilitation efforts where plantations have been established. Thus, there is great need for forest managers to address

  11. Anthelmintic, antimicrobial, antioxidant and cytotoxic activity of Caltha palustris var. alba Kashmir, India.

    Science.gov (United States)

    Mubashir, Sofi; Dar, Mohd Yousuf; Lone, Bashir A; Zargar, M Iqbal; Shah, Wajaht A

    2014-08-01

    The methanolic extract obtained from the root portion of Caltha palustris var. alba was evaluated for its anthelmintic efficacy against gastrointestinal nematodes of sheep under both in vitro and in vivo conditions using worm motility inhibition (WMI) assay and fecal egg count reduction (FECR) assay, respectively. The extract was subjected to antimicrobial activity using agar-well diffusion method against different bacterial strains. In addition the extract was evaluated for cytotoxic and antioxidant activity against cultured THP-1(Leukemia), A-549 (Lung), HCT-15 (Colon), Cervix (HeLa) and PC-3(Prostrate) cell lines by SRB and DPPH radical scavenging assays. The extract used resulted in mean %WMI of 94.44%, as observed when the worms were put in lukewarm buffer for 30 min after exposure to different treatments. The mean mortality index of the sample was 0.95. The lethal concentration (LC50) was 0.11 mg·mL(-1). Cell lines were exposed to concentration of 100 μg·mL(-1) of extract for 48 h, which reduced the viability of these cell lines. The same plant extract also showed 55.58% DPPH radical scavenging activity.

  12. Phylogeny of the "forgotten" cellular slime mold, Fonticula alba, reveals a key evolutionary branch within Opisthokonta.

    Science.gov (United States)

    Brown, Matthew W; Spiegel, Frederick W; Silberman, Jeffrey D

    2009-12-01

    The shared ancestry between Fungi and animals has been unequivocally demonstrated by abundant molecular and morphological data for well over a decade. Along with the animals and Fungi, multiple protists have been placed in the supergroup Opisthokonta making it exceptionally diverse. In an effort to place the cellular slime mold Fonticula alba, an amoeboid protist with aggregative, multicellular fruiting, we sequenced five nuclear encoded genes; small subunit ribosomal RNA, actin, beta-tubulin, elongation factor 1-alpha, and the cytosolic isoform of heat shock protein 70 for phylogenetic analyses. Molecular trees demonstrate that Fonticula is an opisthokont that branches sister to filose amoebae in the genus Nuclearia. Fonticula plus Nuclearia are sister to Fungi. We propose a new name for this well-supported clade, Nucletmycea, incorporating Nuclearia, Fonticula, and Fungi. Fonticula represents the first example of a cellular slime mold morphology within Opisthokonta. Thus, there are four types of multicellularity in the supergroup-animal, fungal, colonial, and now aggregative. Our data indicate that multicellularity in Fonticula evolved independent of that found in the fungal and animal radiations. With the rapidly expanding sequence and genomic data becoming available from many opisthokont lineages, Fonticula may be fundamental to understanding opisthokont evolution as well as any possible commonalities involved with the evolution of multicellularity.

  13. Gota úrica visceral em coruja suindara (Tyto alba de vida livre

    Directory of Open Access Journals (Sweden)

    Indiara dos Santos Sales

    2015-02-01

    Full Text Available A gota úrica é uma doença metabólica caracterizada pela deposição de cristais de urato e ácido úrico em tecidos do corpo, que pode acometer répteis, mamíferos e aves. Neste trabalho, relata-se um caso de achados post mortem compatíveis com gota úrica visceral em um exemplar de coruja suindara (Tyto alba de vida livre. Macroscopicamente, o pericárdio apresentava-se completamente esbranquiçado, espesso e aderido ao miocárdio e o fígado e ambos os rins apresentavam áreas esbranquiçados sugerindo acúmulo de urato. A microscopia do tecido cardíaco e hepático revelou pericardite fibrinosa e perihepatite necrosante respectivamente. Congestão pulmonar e infiltrado inflamatório heterofílico multifocal no parênquima renal também foram visualizados. As alterações encontradas são compatíveis com as descritas na literatura para gota úrica visceral em aves, podendo até serem consideradas como lesões patognomônicas. Este é o primeiro relato de gota úrica visceral em um rapinante de vida livre no Brasil.

  14. Determintation of sulfor containing glucosides in Brassica nepus L. and Sinappis alba L. tissue cultures

    Directory of Open Access Journals (Sweden)

    Suleiman Afsharypuor

    1993-07-01

    Full Text Available In this study, hypocotyls of 5 day old seedlings of Brassica nepus L. and Sinappis alba L.. were explanted onto Murashige and Skoog's revised Tobaco Medium(lcontaining different concentrations of suitable plant growth regulators (a cytokinin and an auxin. In addition to the plant growth regulators, potassium sulfate(in a concentration of 3 m. eq./liter was added to some solid and liquid media in an attempt to raise available sulfate levels, to other medium, L - B- Phenylalanine was added (in a concentration of 30 m. eq./liter to act possibly as a precursor for the biosynthesis of sulfor containing glucoside (glucosinolate. Suspension cell cultures were initiated from callus and grown in the same medium without agar. Calli which were harvested at 20 weeks and cells at 6 weeks after subculture, were dried and examined by capillary GC for the presences of sulfor containing glucosides. The obtained results indicated the absence of such glucosides in the cells and calli.

  15. HPLC-Based Method to Evaluate Kinetics of Glucosinolate Hydrolysis by Sinapis alba Myrosinase1

    Science.gov (United States)

    Vastenhout, Kayla J.; Tornberg, Ruthellen H.; Johnson, Amanda L.; Amolins, Michael W.; Mays, Jared R.

    2014-01-01

    Isothiocyanates (ITCs) are one of several hydrolysis products of glucosinolates, plant secondary metabolites which are substrates for the thioglucohydrolase myrosinase. Recent pursuits toward the development of synthetic, non-natural ITCs have consequently led to an exploration of generating these compounds from non-natural glucosinolate precursors. Evaluation of the myrosinase-dependent conversion of select non-natural glucosinolates to non-natural ITCs cannot be accomplished using established UV-Vis spectroscopic methods. To overcome this limitation, an alternative HPLC-based analytical approach was developed where initial reaction velocities were generated from non-linear reaction progress curves. Validation of this HPLC method was accomplished through parallel evaluation of three glucosinolates with UV-Vis methodology. The results of this study demonstrate that kinetic data is consistent between both analytical methods and that the tested glucosinolates respond similarly to both Michaelis–Menten and specific activity analyses. Consequently, this work resulted in the complete kinetic characterization of three glucosinolates with Sinapis alba myrosinase, with results that were consistent with previous reports. PMID:25068719

  16. Misunderstanding opportunities: (post-resettlement issues in the Recea neighbourhood of Alba Iulia

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    Cătălin Buzoianu

    2013-06-01

    Full Text Available Although its gold mining project has been locked in public debates and permit reviews for over a decade, a Canadian-Romanian company privately negotiated with the inhabitants of Roşia Montană commune, Romania, to buy their households and lands, and resettle them in a specially built neighbourhood in the city of Alba Iulia. This paper suggests that while the paternalistic character of resettlement has allowed resettlers to partially keep their group identity, and partially to reconstruct it in relation with the host community, it was also based on a misunderstanding of the relationship between resettlers and the organiser of resettlement. Drawing on field research, the resettlement was studied as a “continuous process” spanning three years (2010-12, during which this paper identifies (1 the changes in lifestyle, (2 the mechanisms of community regeneration, and (3 post-resettlement initiatives of resettlers. Although greater living costs (utility bills, real estate taxes, transportation and unemployment seem to be balanced by better living conditions and greater educational opportunities for their children, the ambivalent paternalistic aspect of the resettlement has negatively influenced the development of the new community. While at first community issues were unsuccessfully addressed to the company, recent public improvement initiatives by resettlers have caused tensions between the two sides.

  17. The potential of biomonitoring of air quality using leaf characteristics of white willow (Salix alba L.).

    Science.gov (United States)

    Wuytack, Tatiana; Verheyen, Kris; Wuyts, Karen; Kardel, Fatemeh; Adriaenssens, Sandy; Samson, Roeland

    2010-12-01

    In this study, we assess the potential of white willow (Salix alba L.) as bioindicator for monitoring of air quality. Therefore, shoot biomass, specific leaf area, stomatal density, stomatal pore surface, and stomatal resistance were assessed from leaves of stem cuttings. The stem cuttings were introduced in two regions in Belgium with a relatively high and a relatively low level of air pollution, i.e., Antwerp city and Zoersel, respectively. In each of these regions, nine sampling points were selected. At each sampling point, three stem cuttings of white willow were planted in potting soil. Shoot biomass and specific leaf area were not significantly different between Antwerp city and Zoersel. Microclimatic differences between the sampling points may have been more important to plant growth than differences in air quality. However, stomatal pore surface and stomatal resistance of white willow were significantly different between Zoersel and Antwerp city. Stomatal pore surface was 20% lower in Antwerp city due to a significant reduction in both stomatal length (-11%) and stomatal width (-14%). Stomatal resistance at the adaxial leaf surface was 17% higher in Antwerp city because of the reduction in stomatal pore surface. Based on these results, we conclude that stomatal characteristics of white willow are potentially useful indicators for air quality.

  18. Evolutionary conservation of Kv3.1 in the barn owl Tyto alba.

    Science.gov (United States)

    Kullmann, Lars; Schlüter, Tina; Wagner, Hermann; Nothwang, Hans Gerd

    2013-01-01

    For prey capture in the dark, the barn owl Tyto alba has evolved into an auditory specialist with an exquisite capability of sound localization. Adaptations include asymmetrical ears, enlarged auditory processing centers, the utilization of minute interaural time differences, and phase locking along the entire hearing range up to 10 kHz. Adaptations on the molecular level have not yet been investigated. Here, we tested the hypothesis that divergence in the amino acid sequence of the voltage-gated K(+) channel Kv3.1 contributes to the accuracy and high firing rates of auditory neurons in the barn owl. We therefore cloned both splice variants of Kcnc1, the gene encoding Kv3.1. Both splice variants, Kcnc1a and Kcnc1b, encode amino acids identical to those of the chicken, an auditory generalist. Expression analyses confirmed neural-restricted expression of the channel. In summary, our data reveal strong evolutionary conservation of Kcnc1 in the barn owl and point to other genes involved in auditory specializations of this animal. The data also demonstrate the feasibility to address neuroethological questions in organisms with no reference genome by molecular approaches. This will open new avenues for neuroethologists working in these organisms.

  19. Evidences of local adaptation in quantitative traits in Prosopis alba (Leguminosae).

    Science.gov (United States)

    Bessega, C; Pometti, C; Ewens, M; Saidman, B O; Vilardi, J C

    2015-02-01

    Signals of selection on quantitative traits can be detected by the comparison between the genetic differentiation of molecular (neutral) markers and quantitative traits, by multivariate extensions of the same model and by the observation of the additive covariance among relatives. We studied, by three different tests, signals of occurrence of selection in Prosopis alba populations over 15 quantitative traits: three economically important life history traits: height, basal diameter and biomass, 11 leaf morphology traits that may be related with heat-tolerance and physiological responses and spine length that is very important from silvicultural purposes. We analyzed 172 G1-generation trees growing in a common garden belonging to 32 open pollinated families from eight sampling sites in Argentina. The multivariate phenotypes differ significantly among origins, and the highest differentiation corresponded to foliar traits. Molecular genetic markers (SSR) exhibited significant differentiation and allowed us to provide convincing evidence that natural selection is responsible for the patterns of morphological differentiation. The heterogeneous selection over phenotypic traits observed suggested different optima in each population and has important implications for gene resource management. The results suggest that the adaptive significance of traits should be considered together with population provenance in breeding program as a crucial point prior to any selecting program, especially in Prosopis where the first steps are under development.

  20. La palabra y el aire: Conjuros del alba de Jorge Chen-Sham

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    Carolina Sanabria

    2016-10-01

    Full Text Available El presente artículo analiza el segundo poemario de Jorge Chen-Sham, Conjuros del alba, a la luz de la vinculación directa con el libro anterior, Nocturnos de mar inacabada. Destaca los elementos que guían la lectura de un trabajo muy estructurado porque la organización en ejes temáticos permite brindar una clave de lectura. En este caso la articulación está dada por la invocación de la palabra en su acepción divina como creadora de realidad bajo la forma de conjuros –y en sintonía estructural y lingüística con el discurso bíblico arcaizante, pero sin ceñirse por completo en virtud del carácter pagano de estos–. Pero los alcances de la palabra no se limitan a esta dimensión, puesto que el hablante lírico hace ver también su injerencia como mediador del deseo. Y si el poeta por principio lo que busca es la elevación de su espíritu, se mueve necesariamente en el ámbito del aire –que es el mismo elemento al que pertenece el alba–, donde las cosmogonías ubican el origen del mundo.

  1. Inositol Hexakis Phosphate is the Seasonal Phosphorus Reservoir in the Deciduous Woody Plant Populus alba L.

    Science.gov (United States)

    Kurita, Yuko; Baba, Kei'ichi; Ohnishi, Miwa; Matsubara, Ryosuke; Kosuge, Keiko; Anegawa, Aya; Shichijo, Chizuko; Ishizaki, Kimitsune; Kaneko, Yasuko; Hayashi, Masahiko; Suzaki, Toshinobu; Fukaki, Hidehiro; Mimura, Tetsuro

    2017-09-01

    Seasonal recycling of nutrients is an important strategy for deciduous perennials. Deciduous perennials maintain and expand their nutrient pools by the autumn nutrient remobilization and the subsequent winter storage throughout their long life. Phosphorus (P), one of the most important elements in living organisms, is remobilized from senescing leaves during autumn in deciduous trees. However, it remains unknown how phosphate is stored over winter. Here we show that in poplar trees (Populus alba L.), organic phosphates are accumulated in twigs from late summer to winter, and that IP6 (myo-inositol-1,2,3,4,5,6-hexakis phosphate: phytic acid) is the primary storage form. IP6 was found in high concentrations in twigs during winter and quickly decreased in early spring. In parenchyma cells of winter twigs, P was associated with electron-dense structures, similar to globoids found in seeds of higher plants. Various other deciduous trees were also found to accumulate IP6 in twigs during winter. We conclude that IP6 is the primary storage form of P in poplar trees during winter, and that it may be a common strategy for seasonal P storage in deciduous woody plants. © The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  2. THE CHLOROSODIUM MINERAL WATERS IN ALBA COUNTY, LASTING TOURISTIC PROTECTION AND CAPITALIZATION

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    N. CIANGĂ

    2013-03-01

    Full Text Available This study represents a continuation of the research and assessment of the resources of chlorosodium mineral waters on the territory of the Transylvanian Depression, especially regarding the emersion of salty springs, which are still not to be found in the literature of specialty.Therefore, this time, Alba County will be analyzed, a county in which certain researches have been made previously. Field research could also be added, representing the only possibility to update the data regarding the existent salty springs.The first already known examples are the chlorosodium mineral waters billeted in lakes situated in Ocna Mureş. As locations with chlorosodium mineral waters which appear at the surface under the form of salty springs with a salinity exceeding 1 g/l, as compared to the specified ones, Ocnişoara, Cetatea de Baltă, Petrisat - Blaj, Crăciunelu de Jos, Secăşel, Dumitra and Draşov are to be mentioned.As far as the protection of these resources is concerned, the main debated issues are related to phenomena such as their clogging and sweetening, which determines many springs of chlorosodium mineral waters to become ephemeral.

  3. Efficacy of 308-nm xenon chloride excimer laser in pityriasis alba.

    Science.gov (United States)

    Al-Mutairi, Nawaf; Hadad, Ahmed Al

    2012-04-01

    Pityriasis alba (PA) is the most common cause of facial hypopigmentation presenting to the dermatologist. The objective of the current study was to study the effect of the 308-nm excimer laser in the treatment of PA. Twelve patients with 37 PA patches were enrolled in this study. The lesions were treated using the 308-nm excimer laser twice a week for 12 weeks. The hypopigmented areas were evaluated at baseline and at weeks 0, 3, 6, and 12 for scaling, hypopigmentation, and pruritus on a 4-point scale (0 = none to 3 = severe). All adverse effects were recorded. There were seven male and five female participants in (aged 5-21 years), with skin type III to V. After 1 month of laser therapy, the clinical scores were significantly lower than at baseline. Similar decreases were observed for the scaling and pruritus scores. Uneven skin color improved by the third week, and near-complete resolution was noticed by the end of 3 months. No serious or unpleasant side-effects were observed, and all patients completed the 12-week treatment. Patients were satisfied or very satisfied with the treatment. The 308-nm excimer laser is an effective therapeutic option for PA. © 2011 by the American Society for Dermatologic Surgery, Inc. Published by Wiley Periodicals, Inc.

  4. NOTES ON A STATUE FROM THE COLLECTIONS OF THE NATIONAL MUSEUM OF UNION FROM ALBA IULIA

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    Andreea Almăjanu

    2016-04-01

    Full Text Available This study proposes to re-evaluate a marble statue from the old collections of the National Museum of Union Alba Iulia. It was discovered by A. Cserni during excavations carried out at the Palace of the Governors of Roman Dacia, in 1898. It has been repeatedly published by Á. Hekler and Al. Diaconescu. While the latter author established dating and iconographic prototype of the statue, we believe that further details as discovery place and context, depiction, iconographic attribution and role of this work of art can be offered. Presence of a follower at the feet of the divinity, more precisely the right foot of a character – child, conveys us the idea of depiction of Eros, god of love. Usually, this is depicted beside nude or seminude Venus, and sculptural representation from Apulum is somehow different from iconographic point of view. As we see it, taking into consideration the discovery place, artefacts resulted from the same excavation, artistic eclecticism, religious iconographic influences and official politics of the Roman state, we believe we are dealing with rather an unusual statue of Venera as manner of representation or an image of an empress, wife of a governor or member of a family of municipal authorities from Apulum.

  5. Anesthetic induction and recovery of Hippocampus reidi exposed to the essential oil of Lippia alba

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    Mauro Alves da Cunha

    Full Text Available The aim of this study was to identify the times of anesthetic induction and recovery in slender seahorses (Hippocampus reidi that were exposed to the essential oil of Lippia alba (EO, as well as the efficacy of EO as a stress-reducing agent in the transport of this species. Slender seahorses were placed in 1-L aquaria containing different concentrations of EO (0, 10, 20, 50, 150, 300 and 450 µL L-1, and after induction, fish were transferred to aquaria that were free of anesthetic to evaluate their recovery time. In an additional experiment, slender seahorses were transported in plastic bags with 15 µL L-1 of EO for 4 or 24 h. The increased concentration of EO proportionally decreased the time required for the induction of anesthesia. EO treatment (15 µL L-1 inhibited the increase in blood glucose levels that was provoked by transportation for 4 or 24 h. Transportation for 24 h also decreased the number of lymphocytes and increased the neutrophil count, and these effects were avoided with the addition of EO to the water. These results demonstrate that EO was effective as an anesthetic at concentrations of 10-20 µL L-1 for slight sedation and transport and at 150 µL L-1 for deep anesthesia in the slender seahorse.

  6. Albas y alboradas en el cancionero tradicional mexicano: herencia y cambio

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    Masera, Mariana

    1999-12-01

    Full Text Available The roots of some erotic motives in Mexican traditional songs in Spanish could be traced to the Medieval Popular Hispanic songs, as has been said by scholars. But, until now, nobody has studied in depth the relationship and diversity between the Mexican cancionero and old Spanish songs. In this article I shall analize the preservation of the Dawn motif, and all those associated with it, in Mexican Contemporary Songs and variation.Los antecedentes de algunos motivos eróticos de la poesía tradicional mexicana contemporánea en español se pueden rastrear hasta las canciones populares hispánicas de la Edad Media. Sin embargo, aún hoy no se han estudiado en profundidad las relaciones y desencuentros entre la canción popular mexicana y la antigua canción peninsular; por ello, este trabajo se propone como un primer análisis. El estudio resalta la originalidad de las coplas mexicanas a través de un estudio comparativo del motivo del alba.

  7. Sequence and expression characteristics of a nuclear-encoded chloroplast sigma factor from mustard (Sinapis alba).

    Science.gov (United States)

    Kestermann, M; Neukirchen, S; Kloppstech, K; Link, G

    1998-06-01

    Plant chloroplasts contain transcription factors that functionally resemble bacterial sigma factors. We have cloned the full-length cDNA from mustard (Sinapis alba) for a 53 kDa derived polypeptide that contains similarity to regions 1.2-4.2 of sigma70-type factors. The amino acid sequence at the N-terminus has characteristics of a chloroplast transit peptide. An in vitro synthesized polypeptide containing this region was shown to be imported into the chloroplast and processed. The recombinant factor lacking the N-terminal extension was expressed in Escherichia coli and purified. It confers the ability on E.coli core RNA polymerase to bind specifically to a DNA fragment that contains the chloroplast psbA promoter. Transcription of the psbA template by E.coli core enzyme in the presence of recombinant SIG1 results in enhanced formation of transcripts of the size expected for correct initiation at the in vivo start site. Together, these data suggest that the mature protein acts as one of the chloroplast transcription factors in mustard. RNA gel blot hybridization reveals a transcript at approximately 1.8 kb, which is more abundant in light-grown than in dark-grown mustard seedlings.

  8. Prosopis alba exudate gum as excipient for improving fish oil stability in alginate-chitosan beads.

    Science.gov (United States)

    Vasile, Franco Emanuel; Romero, Ana María; Judis, María Alicia; Mazzobre, María Florencia

    2016-01-01

    The aim of the present work was to employ an exudate gum obtained from a South American wild tree (Prosopis alba), as wall material component to enhance the oxidative stability of fish oil encapsulated in alginate-chitosan beads. For this purpose, beads were vacuum-dried and stored under controlled conditions. Oxidation products, fatty acid profiles and lipid health indices were measured during storage. Alginate-chitosan interactions and the effect of gum were manifested in the FT-IR spectra. The inclusion of the gum in the gelation media allowed decreasing the oxidative damage during storage in comparison to the free oil and alginate-chitosan beads. The gum also improved wall material properties, providing higher oil retention during the drying step and subsequent storage. Fatty acids quality and lipid health indices were widely preserved in beads containing the gum. Present results showed a positive influence of the gum on oil encapsulation and stability, being the main mechanism attributed to a physical barrier effect.

  9. Genetic characterization of fast-growing rhizobia able to nodulate Prosopis alba in North Spain.

    Science.gov (United States)

    Iglesias, Olga; Rivas, Raúl; García-Fraile, Paula; Abril, Adriana; Mateos, Pedro F; Martinez-Molina, Eustoquio; Velázquez, Encarna

    2007-12-01

    Prosopis is a Mimosaceae legume tree indigenous to South America and not naturalized in Europe. In this work 18 rhizobial strains nodulating Prosopis alba roots were isolated from a soil in North Spain that belong to eight different randomly amplified polymorphic DNA groups phylogenetically related to Sinorhizobium medicae, Sinorhizobium meliloti and Rhizobium giardinii according to their intergenic spacer and 16S rRNA gene sequences. The nodC genes of isolates close to S. medicae and S. meliloti were identical to those of S. medicae USDA 1,037(T) and S. meliloti LMG 6,133(T) and accordingly all these strains were able to nodulate both alfalfa and Prosopis. These nodC genes were phylogenetically divergent from those of the isolates close to R. giardinii that were identical to that of R. giardinii H152(T) and therefore all these strains formed nodules in common beans and Prosopis. The nodC genes of the strains isolated in Spain were phylogenetically divergent from that carried by Mesorhizobium chacoense Pr-5(T) and Sinorhizobium arboris LMG 1,4919(T) nodulating Prosopis in America and Africa, respectively. Therefore, Prosopis is a promiscuous host which can establish symbiosis with strains carrying very divergent nodC genes and this promiscuity may be an important advantage for this legume tree to be used in reforestation.

  10. Formulation and sensory evaluation of Prosopis alba (Algarrobo) pulp cookies with increased iron and calcium dialyzabilities.

    Science.gov (United States)

    Bernardi, C; Drago, S; Sabbag, N; Sanchez, H; Freyre, M

    2006-03-01

    Prosopis alba (algarrobo) is an important indigenous specie, which fruits are used as food and feed since ancient times. Cookies containing algarrobo pulp (AP) with increased iron and calcium availabilities were formulated and sensory evaluated. AP is preferred as food ingredient because of its high sugar content and pleasant flavour. Formulated cookies mean proximal composition was 8.9 g/100 g protein, 7.2 g/100 g dietary fiber, 25 g/100 g total sugar, and 18.5 g/100 g crude fat with iron and calcium contents 30 ppm and 340 ppm, respectively. Ascorbic (AA) and citric (CA) acids at different mM acid: mM Fe were added in order to increase mineral availabilities being evaluated by an in vitro method. Those ratios were 5:1 and 10:1 for AA:Fe whereas for CA:Fe were 50:1 and 100:1 and combinations of them. After chosen the best AA:Fe and CA:Fe ratios (5:1 and 50:1, respectively), sensory evaluation with trained sensory panel and a consumer acceptability test with one hundred and seventy untrained judges were carried out. Acceptability test showed that 77.65% of the people ( 50 years old 15.89%) tasting final formulated cookies indicated that they "like very much" or "moderately like" and there were not consumers rejecting them.

  11. Analysis of F1 hybrid and BC1 monosomic alien addition line plants from Brassica oleracea × Sinapis alba by GISH

    Institute of Scientific and Technical Information of China (English)

    WEI Wenhui; ZHANG Sufeng; LI Jun; WANG Lijun; CHEN Bo; FANG Xiaoping; WANG Zhuan; LUO Lixia

    2006-01-01

    Sterile and semi-fertile F1 plants were obtained by intergeneric sexual hybridization between paternal Brassica oleracea var. alboglabra (genome CC, 2n=18) and maternal Sinapis alba (genome SS, 2n=24), BC1 plants were obtained by backcrossing between paternal B. oleracea and maternal semi-fertile F1 plants. Genomic in situ hybridization (GISH) combined with dual-colour fluorescence in situ hybridization (dcFISH) showed that sterile F1 plants contained 21 chromosomes consisting of one B. oleracea chromosome set and one S.alba chromosome set, belonging to expected hybrids,and semi-fertile F1 plants contained 30 chromosomes consisting of two B. oleracea chromosome sets and one S. alba chromosome set. It is obvious that the semi-fertile F1 plants belong to unexpected hybrids.1 -3 trivalents were detected at meiotic metaphase I of semi-fertile F1 pollen mother cells (PMCs). Different separation ratios of S chromosomes were detected at anaphase I. A monosomic alien addition line(MAAL) was identified by GISH-dcFISH from BC1plants; it contained 19 chromosomes consisting of 18 C chromosomes and 1 S chromosome. At meiotic metaphase I, 9 divalents from B. oleracea and one univalent from S. alba could be detected. Sometimes,one putative C-S trivalent could also be detected.The achievement of B. oleracea-S, alba monosomic alien addition lines lays a foundation for gene introgression, location and cloning.

  12. Helminth communities of two species of piscivorous birds, Ardea alba (Linnaeus) and Nyctanassa violacea (Gmelin) (Ciconiiformes: Ardeidae), in two coastal lagoons from Guerrero state, Mexico.

    Science.gov (United States)

    Violante-González, Juan; Monks, Scott; Gil-Guerrero, Salvador; Rojas-Herrera, Agustín A; Flores-Rodríguez, Pedro

    2012-07-01

    The composition and species richness in helminth communities of two species of heron, Ardea alba and Nyctanassa violacea, in two coastal lagoons from Guerrero, Mexico were examined. Nineteen species of helminth (7,804 individuals) were identified in 43 adult birds: 15 digeneans, 1 acanthocephalan, 1 cestode, and 2 nematodes. Eight species co-occurred in herons of both species and lagoons. The prevalence values of seven species and the mean abundance of five species varied significantly between species of birds and between lagoons. The heterophyid, Ascocotyle (Phagicola) longa, was the helminth numerically dominant in the helminth community of A. alba in both lagoons, while the cestode, Parvitaenia cochlearii, dominated the community of N. violacea. At the component community level, species richness varied significantly: 10 species in A. alba from Coyuca to 16 in N. violacea (Tres Palos). All of the birds examined were infected with helminth parasites: three to seven species per host in A. alba from Coyuca, and two to eight species in A. alba and N. violacea from Tres Palos. The results indicate that even though species composition was similar between both species of heron, the structure of their communities was not the same. Differences in the feeding behavior of the birds (day/night habits), as well as local differences in the abundance of species of fish, and infection levels of helminths in each lagoon are suggested as being responsible for the variations registered in the structure of the helminth communities.

  13. [Qualitative and quantitative analysis of major constituents of Paeoniae Radix Alba and Paeoniae Radix Rubra by HPLC-DAD-Q-TOF-MS/MS].

    Science.gov (United States)

    Liu, Jie; Chen, Lin; Fan, Cai-rong; Li, Huang; Huang, Ming-qing; Xiang, Qing; Xu, Wen; Xu, Wei; Chu, Ke-dan; Lin, Yu

    2015-05-01

    In order to explore the differences of chemical constituents of Paeoniae Radix Alba and Paeoniae Radix Rubra, a qualitative analytical method of liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF-MS/MS) was developed for identification of multi-constituents and an HPLC-DAD analytical method was developed for simultaneously determining 14 major compounds (gallic acid, protocatechuic acid, paeoniflorin sulfonate, protocatechuic aldehyde, methyl gallate, oxypaeoniflorin, catechin, albiflorin, and paeoniflorin, ethyl gallate, benzoic acid, pentagaloylglucose, benzoyl-paeoniflorin, and paeonol) in Paeoniae Radix Alba and Paeoniae Radix Rubra. Q-TOF/MS qualitative analysis was performed under negative ion mode and inferred 38 components of Paeoniae Radix Alba and 30 components of Paeoniae Radix Rubra. HPLC-DAD quantitative method result showed the contents of 8 ingredients were different between Paeoniae Radix Alba and Paeoniae Radix Rubra. The results indicated that the new approach was applicable in qualitative and quantitative quality control of Paeoniae Radix Alba and Paeoniae Radix Rubra.

  14. Avaliação toxicológica pré-clínica do chá das folhas de Morus nigra L. (Moraceae) Pre-clinical toxicological evaluation of tea from the leaves of Morus nigra L. (Moraceae)

    OpenAIRE

    Oliveira,A.C.B.; Oliveira, A.P.; Guimarães,A.L.; R A Oliveira; Silva, F.S.; S.A.G.B. Reis; Ribeiro,L.A.A.; Almeida,J.R.G.S.

    2013-01-01

    O objetivo desse estudo foi realizar um ensaio toxicológico pré-clínico para analisar a toxicidade do chá das folhas de Morus nigra L. (Moraceae). A toxicidade subcrônica do chá (CF-Mn) foi avaliada durante 30 dias por via oral em ratos. Ao grupo controle foi administrado água, para comparação. Durante o período experimental foi avaliada a presença de sinais de toxicidade, variação do peso corporal, e o consumo de líquido e alimento. Ao final do experimento o sangue dos animais foi retirado p...

  15. Fungitoxicty of Bidens pilosa, Thymus vulgaris, Lippia alba and Rosmarinus officinalis in the in vitro development of phytophatogens fungi / Fungitoxidade de Bidens pilosa, Thymus vulgaris, Lippia alba e Rosmarinus officinalis no desenvolvimento in vitro de fungos fitopatogênicos

    Directory of Open Access Journals (Sweden)

    José Renato Stangarlin

    2009-07-01

    Full Text Available The fungitoxicity of aqueous crude extracts (ACE of Bidens pilosa, Thymus vulgaris, Lippia alba and Rosmarinus officinalis was evaluated in vitro on development of Alternaria alternata, Colletotrichum graminicola, Rhizoctonia solani and Sclerotium rolfsii. The active ACE were incorporated to Potato- Dextrose-Agar (PDA alone or in mixture, at 0%, 1%, 5%, 10%, 15%, 25% and 50% concentrations. Measurement in fungal colonies was taken daily until the control treatment covered 2/3 of the culture media. The mixtures of T. vulgaris with B. pilosa did not have positive effect because the ACE of B. pilosa did not reduce the fungal growth of R. solani and in mixture inhibited the effect ACE of T. vulgaris. However, the mixture of ACEs of R. officinalis and L. alba produced better results than isolated ACEs, with 60% of growth inhibition of A. alternata. The ACE of T. vulgaris inhibited the growth of C. graminicola at 97%. The results show that ACEs have significative fungitoxicity in the evaluated phythopathogens.Avaliou-se in vitro, a atividade fungitóxica dos extratos brutos aquosos (EBA em diferentes concentrações isolados e em misturas de Bidens pilosa, Thymus vulgaris, Lippia alba e Rosmarinus officinalis no desenvolvimento dos fungos Alternaria alternata, Colletotrichum graminicola, Rhizoctonia solani e Sclerotium rolfsii. A avaliação foi realizada incorporando os extratos brutos aquosos (EBAs ao meio BDA isolados e em mistura, nas concentrações 0%, 1%, 5%, 10%, 15%, 25% e 50%. Foram feitas medições diárias das colônias fúngicas até o momento em o tratamento controle cobriu 2/3 da superfície do meio de cultura. Verificou-se que a mistura de T. vulgaris com B. pilosa não teve efeito positivo, pois o EBA isolado de B. pilosa não reduziu o crescimento do fungo R. solani e em mistura reduziu o efeito do EBA de T. vulgaris. Porém, o EBAs de R. officinalis e L. alba em mistura levaram a melhores resultados, reduzindo em até 60% o

  16. Antioxidant capacity and HPLC-DAD-MS profiling of Chilean peumo (Cryptocarya alba) fruits and comparison with German peumo (Crataegus monogyna) from southern Chile.

    Science.gov (United States)

    Simirgiotis, Mario J

    2013-02-05

    Liquid chromatography (LC) coupled with UV detection and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) was used for the generation of chemical fingerprints and the identification of phenolic compounds in peumo fruits and aerial parts from southern Chile. Thirty three compounds (19 of these detected in C. alba and 23 in C. monogyna) were identified, mainly flavonoid glycosides, phenolic acids, anthocyanins and flavonoid aglycons. Total phenolic content and total flavonoid content was measured for both species, and were higher in the extracts from C. monogyna fruits and aerial parts than extracts from C. alba. The fruits of Cryptocarya alba (Chilean peumo) presented high antioxidant capacity (9.12 ± 0.01 mg/mL in the DPPH assay), but was three times lower to that of Crataegus monogyna (German peumo) (3.61 ± 0.01 mg/mL in the DPPH assay).

  17. Antioxidant Capacity and HPLC-DAD-MS Profiling of Chilean Peumo (Cryptocarya alba Fruits and Comparison with German Peumo (Crataegus monogyna from Southern Chile

    Directory of Open Access Journals (Sweden)

    Mario J. Simirgiotis

    2013-02-01

    Full Text Available Liquid chromatography (LC coupled with UV detection and electrospray ionization (ESI tandem mass spectrometry (MS/MS was used for the generation of chemical fingerprints and the identification of phenolic compounds in peumo fruits and aerial parts from southern Chile. Thirty three compounds (19 of these detected in C. alba and 23 in C. monogyna were identified, mainly flavonoid glycosides, phenolic acids, anthocyanins and flavonoid aglycons. Total phenolic content and total flavonoid content was measured for both species, and were higher in the extracts from C. monogyna fruits and aerial parts than extracts from C. alba. The fruits of Cryptocarya alba (Chilean peumo presented high antioxidant capacity (9.12 ± 0.01 mg/mL in the DPPH assay, but was three times lower to that of Crataegus monogyna (German peumo (3.61 ± 0.01 mg/mL in the DPPH assay.

  18. Integración latinoamericana en la Web: Diseño de un sitio en Internet para la difusión del ALBA

    Directory of Open Access Journals (Sweden)

    Raquel Álvarez

    2008-12-01

    Full Text Available The Bolivarian Alternative for the Peoples of the Americas (ALBA, is presented as a proposal to help solve social crises of the people and continue efforts to achieve unity, especially among the countries linked by geography, history or culture. The design of a Web site as a strategy for the dissemination of this proposal is justified largely by the high penetration has had Internet in recent times in Latin America and the world. In the study, it was applied interviews to a group of experts (journalists, lawyers, researchers and university teachers and social promoters, who provided different views the knowledge of ALBA. The Web site that was designed to disseminate the proposed integration ALBA has a presence on the Internet under the domain www.alternativabolivariana.com.ve, and it has a navigation menu that includes an information forum, download, contact and interviews.

  19. ¿Hacia una Nueva Arquitectura Financiera Regional? Problemas y perspectivas de la cooperación monetaria en el ALBA-TCP

    Directory of Open Access Journals (Sweden)

    Benzi, D.

    2016-05-01

    Full Text Available This paper addresses the experiences of the monetary and financial cooperation carried out within the framework of ALBA-TCP. The first part describes the context of global crisis and emerging multipolarity in which the debate on the reform of the International Financial Architecture (IFA and regional monetary initiatives are inserted. The second part revises the Latin American initiative of a New Regional Financial Architecture. Later, we analyze the origins, processes and effects of mechanisms implemented within the ALBA-TCP, such as the ALBA Bank and the SUCRE. Finally, the essay concludes by presenting both potential and limitations of these experiences within a regional and global scenario marked by a high degree of uncertainty that, paradoxically so far, seems to delay and inhibit rather than consolidate the strengthening of a New Regional Financial Architecture.

  20. Fractional CO 2 laser as an effective modality in treatment of striae alba in skin types III and IV

    Directory of Open Access Journals (Sweden)

    Farahnaz Fatemi Naein

    2012-01-01

    Full Text Available Context: Rapid stretching of the skin over the weak connective tissue leads to development of striae distensae. Recently, researchers have shown special interest towards use of fractional photothermolysis in treatment of striae and several studies have shown its usefulness. Our aim was to assess the efficacy of Fractional CO 2 laser in treatment of striae alba. Materials and Methods: A randomized clinical trial was carried out in female patients with striae alba. Ninety two striae were randomly selected and divided into two groups. Five sessions of laser resurfacing, were performed in Group 1, every 2-4 weeks. Group 2 was treated with 10% glycolic acid+0.05% tretinoin cream nightly during the study. Photographs were taken from the striae before and two weeks after the end of treatment. Mean surface area of striae compared between two groups. Patients′ views regarding the degree of improvement were assessed via visual analogue scale (VAS. Results: Forty six striae in Group 1 underwent laser resurfacing and 46 matched striae in Group 2, were treated with topical cream. Mean difference of striae surface area, was significantly decreased after treatment in Group 1 (-37.1±15.6 cm 2 in comparison with Group 2 (-7.9±9 cm 2 (P value >0.001. Mean VAS was significantly higher in Group 1 (3.05±0.74 compared to Group 2 (0.63±0.66 (P value >0.001. Conclusions: Fractional photothermolysis via Fractional CO 2 laser seems to be an effective method for treatment of striae alba.