WorldWideScience

Sample records for amines du peptide

  1. An amine-derivatized, DOTA-loaded polymeric support for Fmoc Solid Phase Peptide Synthesis

    OpenAIRE

    Yoo, Byunghee; Sheth, Vipul R.; Pagel, Mark D.

    2009-01-01

    An amine-derivatized DOTA has been used to modify the surface of a polymeric support for conventional Solid Phase Peptide Synthesis (SPPS) following standard Fmoc chemistry methods. This methodology was used to synthesize a peptide-DOTA conjugate that was demonstrated to be a PARACEST MRI contrast agent. Therefore, this synthesis methodology can facilitate Fmoc SPPS of molecular imaging contrast agents.

  2. Selective acylation of primary amines in peptides and proteins

    NARCIS (Netherlands)

    Abello, N.; Kerstjens, H.A.M.; Postma, D.S; Bischoff, Rainer

    2007-01-01

    N-hydroxysuccinimide (NHS) esters are derivatizing agents that target primary amine groups. However, even a small molar excess of NHS may lead to acylation of hydroxyl-containing amino acids as a side reaction. We report a straightforward method for the selective removal of ester-linked acyl groups

  3. Design of cationic microspheres based on aminated gelatin for controlled release of peptide and protein drugs.

    Science.gov (United States)

    Morimoto, Kazuhiro; Chono, Sumio; Kosai, Tadashi; Seki, Toshinobu; Tabata, Yasuhiko

    2008-02-01

    Two different types of cationized microspheres based on a native cationic gelatin (NGMS) and aminated gelatin with ethylendiamine (CGMS) were investigated for the controlled release of three model acidic peptide/protein drugs with different molecular weights (MWs) and isoelectric points (IEPs). Recombinant human (rh)-insulin (MW: 5.8 kDa, IEP: 5.3), bovine milk lactoalbumin, BMLA (MW: 14 kDa, IEP: 4.3), and bovine serum albumin (BSA MW: 67 kDa, IEP: 4.9) were used as model acidic peptide/protein drugs. The in vitro release profiles of these acidic peptide/protein drugs from NGMS and CGMS were compared and different periods of cross-linking were obtained. The slower release of these acidic peptide/protein drugs from CGMS compared with those from NGMS with cross-linking for 48 hr. was caused by the suppression of burst release during the initial phase. The degree of suppression of burst release of the three peptide/protein drugs during the initial phase by CGMS was in the following order: (rh)-insulin > BMLA > BSA. The release of insulin with a lower molecular weight from CGMS was particularly suppressed compared with the other two drugs with higher molecular weights in the initial phase. The control of the release rate of acidic peptide/protein drugs from gelatin microsphere can be achieved by amination of gelatin. Therefore, CGMS is useful for the controlled release of acidic peptide/ protein drugs.

  4. Etude du métabolisme des amines biogènes chez les bactéries lactiques du vin

    OpenAIRE

    Bonnin-Jusserand, Maryse

    2011-01-01

    Les amines biogènes sont des composés allergènes, notamment rencontrés dans les produits fermentés tel que le vin. Les bactéries lactiques du vin, dont Oenococcus oeni, le principal acteur de la fermentation malolactique, sont capables de produire ces molécules, à partir de précurseurs azotés. Afin de réduire la teneur en amines biogènes, il est nécessaire de comprendre le rôle de cette production, chez les souches impliquées dans la synthèse de ces métabolites. Le projet européen BiamFood FP...

  5. Peptide Macrocycles Featuring a Backbone Secondary Amine: A Convenient Strategy for the Synthesis of Lipidated Cyclic and Bicyclic Peptides on Solid Support

    DEFF Research Database (Denmark)

    Oddo, Alberto; Münzker, Lena; Hansen, Paul Robert

    2015-01-01

    A convenient strategy for the on-resin synthesis of macrocyclic peptides (3- to 13-mers) via intramolecular halide substitution by a diamino acid is described. The method is compatible with standard Fmoc/tBu SPPS and affords a tail-to-side-chain macrocyclic peptide featuring an endocyclic seconda...... amine. This functional group is still reactive toward acylation, allowing for the continuation of the synthesis. An application to the synthesis of lipidated cyclic and bicyclic antimicrobial peptides is presented....

  6. Bioactive Molecules Released in Food by Lactic Acid Bacteria: Encrypted Peptides and Biogenic Amines

    Science.gov (United States)

    Pessione, Enrica; Cirrincione, Simona

    2016-01-01

    Lactic acid bacteria (LAB) can produce a huge amount of bioactive compounds. Since their elective habitat is food, especially dairy but also vegetal food, it is frequent to find bioactive molecules in fermented products. Sometimes these compounds can have adverse effects on human health such as biogenic amines (tyramine and histamine), causing allergies, hypertensive crises, and headache. However, some LAB products also display benefits for the consumers. In the present review article, the main nitrogen compounds produced by LAB are considered. Besides biogenic amines derived from the amino acids tyrosine, histidine, phenylalanine, lysine, ornithine, and glutamate by decarboxylation, interesting peptides can be decrypted by the proteolytic activity of LAB. LAB proteolytic system is very efficient in releasing encrypted molecules from several proteins present in different food matrices. Alpha and beta-caseins, albumin and globulin from milk and dairy products, rubisco from spinach, beta-conglycinin from soy and gluten from cereals constitute a good source of important bioactive compounds. These encrypted peptides are able to control nutrition (mineral absorption and oxidative stress protection), metabolism (blood glucose and cholesterol lowering) cardiovascular function (antithrombotic and hypotensive action), infection (microbial inhibition and immunomodulation) and gut-brain axis (opioids and anti-opioids controlling mood and food intake). Very recent results underline the role of food-encrypted peptides in protein folding (chaperone-like molecules) as well as in cell cycle and apoptosis control, suggesting new and positive aspects of fermented food, still unexplored. In this context, the detailed (transcriptomic, proteomic, and metabolomic) characterization of LAB of food interest (as starters, biocontrol agents, nutraceuticals, and probiotics) can supply a solid evidence-based science to support beneficial effects and it is a promising approach as well to obtain

  7. Bioactive Molecules Released in Food by Lactic Acid Bacteria: Encrypted Peptides and Biogenic Amines.

    Science.gov (United States)

    Pessione, Enrica; Cirrincione, Simona

    2016-01-01

    Lactic acid bacteria (LAB) can produce a huge amount of bioactive compounds. Since their elective habitat is food, especially dairy but also vegetal food, it is frequent to find bioactive molecules in fermented products. Sometimes these compounds can have adverse effects on human health such as biogenic amines (tyramine and histamine), causing allergies, hypertensive crises, and headache. However, some LAB products also display benefits for the consumers. In the present review article, the main nitrogen compounds produced by LAB are considered. Besides biogenic amines derived from the amino acids tyrosine, histidine, phenylalanine, lysine, ornithine, and glutamate by decarboxylation, interesting peptides can be decrypted by the proteolytic activity of LAB. LAB proteolytic system is very efficient in releasing encrypted molecules from several proteins present in different food matrices. Alpha and beta-caseins, albumin and globulin from milk and dairy products, rubisco from spinach, beta-conglycinin from soy and gluten from cereals constitute a good source of important bioactive compounds. These encrypted peptides are able to control nutrition (mineral absorption and oxidative stress protection), metabolism (blood glucose and cholesterol lowering) cardiovascular function (antithrombotic and hypotensive action), infection (microbial inhibition and immunomodulation) and gut-brain axis (opioids and anti-opioids controlling mood and food intake). Very recent results underline the role of food-encrypted peptides in protein folding (chaperone-like molecules) as well as in cell cycle and apoptosis control, suggesting new and positive aspects of fermented food, still unexplored. In this context, the detailed (transcriptomic, proteomic, and metabolomic) characterization of LAB of food interest (as starters, biocontrol agents, nutraceuticals, and probiotics) can supply a solid evidence-based science to support beneficial effects and it is a promising approach as well to obtain

  8. Peptide- and Amine-Modified Glucan Particles for the Delivery of Therapeutic siRNA.

    Science.gov (United States)

    Cohen, Jessica L; Shen, Yuefei; Aouadi, Myriam; Vangala, Pranitha; Tencerova, Michaela; Amano, Shinya U; Nicoloro, Sarah M; Yawe, Joseph C; Czech, Michael P

    2016-03-01

    Translation of siRNA technology into the clinic is limited by the need for improved delivery systems that target specific cell types. Macrophages are particularly attractive targets for RNAi therapy because they promote pathogenic inflammatory responses in a number of important human diseases. We previously demonstrated that a multicomponent formulation of β-1,3-d-glucan-encapsulated siRNA particles (GeRPs) can specifically and potently silence genes in mouse macrophages. A major advance would be to simplify the GeRP system by reducing the number of delivery components, thus enabling more facile manufacturing and future commercialization. Here we report the synthesis and evaluation of a simplified glucan-based particle (GP) capable of delivering siRNA in vivo to selectively silence macrophage genes. Covalent attachment of small-molecule amines and short peptides containing weak bases to GPs facilitated electrostatic interaction of the particles with siRNA and aided in the endosomal release of siRNA by the proton-sponge effect. Modified GPs were nontoxic and were efficiently internalized by macrophages in vitro. When injected intraperitoneally (i.p.), several of the new peptide-modified GPs were found to efficiently deliver siRNA to peritoneal macrophages in lean, healthy mice. In an animal model of obesity-induced inflammation, i.p. administration of one of the peptide-modified GPs (GP-EP14) bound to siRNA selectively reduced the expression of target inflammatory cytokines in the visceral adipose tissue macrophages. Decreasing adipose tissue inflammation resulted in an improvement of glucose metabolism in these metabolically challenged animals. Thus, modified GPs represent a promising new simplified system for the efficient delivery of therapeutic siRNAs specifically to phagocytic cells in vivo for modulation of inflammation responses. PMID:26815386

  9. Peptide- and Amine-Modified Glucan Particles for the Delivery of Therapeutic siRNA.

    Science.gov (United States)

    Cohen, Jessica L; Shen, Yuefei; Aouadi, Myriam; Vangala, Pranitha; Tencerova, Michaela; Amano, Shinya U; Nicoloro, Sarah M; Yawe, Joseph C; Czech, Michael P

    2016-03-01

    Translation of siRNA technology into the clinic is limited by the need for improved delivery systems that target specific cell types. Macrophages are particularly attractive targets for RNAi therapy because they promote pathogenic inflammatory responses in a number of important human diseases. We previously demonstrated that a multicomponent formulation of β-1,3-d-glucan-encapsulated siRNA particles (GeRPs) can specifically and potently silence genes in mouse macrophages. A major advance would be to simplify the GeRP system by reducing the number of delivery components, thus enabling more facile manufacturing and future commercialization. Here we report the synthesis and evaluation of a simplified glucan-based particle (GP) capable of delivering siRNA in vivo to selectively silence macrophage genes. Covalent attachment of small-molecule amines and short peptides containing weak bases to GPs facilitated electrostatic interaction of the particles with siRNA and aided in the endosomal release of siRNA by the proton-sponge effect. Modified GPs were nontoxic and were efficiently internalized by macrophages in vitro. When injected intraperitoneally (i.p.), several of the new peptide-modified GPs were found to efficiently deliver siRNA to peritoneal macrophages in lean, healthy mice. In an animal model of obesity-induced inflammation, i.p. administration of one of the peptide-modified GPs (GP-EP14) bound to siRNA selectively reduced the expression of target inflammatory cytokines in the visceral adipose tissue macrophages. Decreasing adipose tissue inflammation resulted in an improvement of glucose metabolism in these metabolically challenged animals. Thus, modified GPs represent a promising new simplified system for the efficient delivery of therapeutic siRNAs specifically to phagocytic cells in vivo for modulation of inflammation responses.

  10. Cleavable ester linked magnetic nanoparticles for labeling of solvent exposed primary amine groups of peptides/proteins

    Directory of Open Access Journals (Sweden)

    Ujwal S. Patil

    2015-09-01

    Full Text Available Covalent labeling of solvent exposed amino acid residues using chemical reagents/crosslinkers followed by mass spectrometric analysis can be used to determine the solvent accessible amino acids of a protein. A variety of chemical reagents containing cleavable bonds were developed to label abundantly found lysine residues on the surface of protein. To achieve efficient separation of labeled peptides prior to mass spectrometric analysis, magnetic nanoparticles can be decorated with amino acid reactive functional groups and utilized for quick recovery of labeled peptides. [1] In this work, iron oxide magnetic nanoparticles (Fe3O4 MNPs were synthesized by thermal decomposition method and coated with silica (SiO2@Fe3O4 MNPs by reverse micro emulsion approach. The Fe3O4 MNPs and SiO2@Fe3O4 MNPs were characterized by TEM and XRD. The SiO2@Fe3O4 MNPs were further coated with amine groups and conjugated to N-hydroxysuccinimidyl (NHS ester groups via a cleavable ester bond. Fluorescence based qualitative analysis of ester linked NHS ester modified SiO2@Fe3O4 MNPs was performed to confirm the presence of NHS ester group. The active NHS ester sites on the surface of SiO2@Fe3O4 MNPs were determined by depletion approach and found to be 694 active sites per 1 mg of SiO2@Fe3O4 MNPs. Free amine groups of a small peptide, ACTH (4–11 were labeled by ester linked, NHS ester modified SiO2@Fe3O4 MNPs under physiological conditions. Superparamagnetic nature of SiO2@Fe3O4 MNPs allowed quick and efficient magnetic separation of labeled peptides from the solution. The ester bond was further cleaved to separate labeled peptides followed by mass spectrometric analysis. The ester linked, NHS ester modified SiO2@Fe3O4 MNPs introduced a mass shift of 115.09 Da on amine groups of ACTH (4–11, which was confirmed by mass spectrometry.

  11. Bioactive Molecules Released in Food by Lactic Acid Bacteria: Encrypted Peptides and Biogenic Amines

    OpenAIRE

    Pessione, Enrica; Cirrincione, Simona

    2016-01-01

    Lactic acid bacteria (LAB) can produce a huge amount of bioactive compounds. Since their elective habitat is food, especially dairy but also vegetal food, it is frequent to find bioactive molecules in fermented products. Sometimes these compounds can have adverse effects on human health such as biogenic amines (tyramine and histamine), causing allergies, hypertensive crises, and headache. However, some LAB products also display benefits for the consumers. In the present review article, the ma...

  12. Aspects moléculaires et biochimiques des stylicines, peptides multifonctionnels identifiés chez la crevette bleue du Pacifique Litopenaeus stylirostris (Crustacea, Decapoda)

    OpenAIRE

    Rolland, Jean-Luc

    2010-01-01

    Les travaux présentés dans ce mémoire ont été motivés par l’importance économique de l’élevage de la crevette bleue du pacifique Litopenaeus stylirostris dont les fortes mortalités sont principalement dues au développement de maladies bactériennes et virales. Ils ont consisté en la caractérisation des deux premiers membres d’une famille originale de peptides multifonctionnels présents chez les crevettes pénéides, les stylicines. Ces peptides, nommés stylicines 1 et 2, sont des peptides anioni...

  13. Study of the degradation mechanisms of amines used for the capture of CO{sub 2} in industrial fumes; Etude des mecanismes de degradation des amines utilisees pour le captage du CO{sub 2} dans les fumees

    Energy Technology Data Exchange (ETDEWEB)

    Lepaumier, H

    2008-10-15

    Global warming leads to reduce greenhouse gas emissions. Post combustion CO{sub 2} capture with solvent is the most advanced technology to reduce CO{sub 2} emissions in industrial fumes. A major problem associated with chemical absorption of CO{sub 2} using the benchmark ethanolamine (MEA) is solvent degradation through irreversible side reactions with CO{sub 2} and O{sub 2} which leads to numerous harmful impacts to the process: corrosion, solvent loss, foaming, fouling, and viscosity increase. So, developing new amines with higher chemical stability is essential. This work is based on the chemical stability study of 17 different molecules. Their structures have been chosen in order to establish structure-property relationships: alkanolamines, known for gas treatment application (MEA, DEA, MDEA, AMP...), di-amines, and tri-amines without alcohol function. Impact of temperature, CO{sub 2}, and O{sub 2} on degradation has been studied. Strong experimental conditions have been used to observe significant degradation after a 15 days experiment. Separation, identification and quantification of degradation products have been performed by using different testing instructions such as gas chromatography, mass spectrometry, ionic chromatography and NMR. Different mechanisms are proposed to explain most of degradation compounds. Radical reactions (dealkylation, alkylation, ring-closure reactions and piperazinones formation) are involved under O{sub 2} pressure whereas CO{sub 2} induces ionic reactions (dealkylation, alkylation, addition, ring-closure reactions and oxazolidinones or imidazolidinones formation). Large discrepancies of stability are noticed among the different amines. Knowledge of degradation products and reaction mechanisms has thus permitted to establish some relationships between structure and chemical stability: for example, role of the amine function (primary, secondary, tertiary), impact of alkyl chain length between the two amino groups and steric

  14. Graph Machine Based-QSAR Approach for Modeling Thermodynamic Properties of Amines: Application to CO2 Capture in Postcombustion Approche QSAR Graph Machines pour la modélisation des propriétés thermodynamiques des amines : application au captage du CO2 en postcombustion

    Directory of Open Access Journals (Sweden)

    Porcheron F.

    2013-03-01

    Full Text Available Amine scrubbing is usually considered as the most efficient technology for CO2 mitigation through postcombustion Carbon Capture and Storage (CCS. However, optimization of the amine structure to improve the solvent properties requires to sample a large number of possible candidates and hence to gather a large amount of experimental data. In this context, the use of QSAR (Quantitative Structure Activity Relationship statistical modeling is a powerful tool as it performs a mapping of a set of input vectors (i.e. the characteristics or the properties of the molecules under consideration to a set of output vectors (i.e. their targeted properties. In this work, we used a high throughput screening experimental device to measure CO2 solubility data on a set of 46 amine aqueous solutions. Absorption isotherms are represented using a thermodynamic model based on two thermodynamic constants, pKa*  and pKc* , accounting for the main chemical reactions occurring in the liquid phase between amine and CO2. Then, we used a statistical approach named Graph Machines at the same time to cluster the molecules and to model the variation of the acidity constant pKa*  as a function of the molecular structure. The originality of our approach is the use of graphs to represent molecules in multidimensional spaces and simultaneously construct predictive models of their physicochemical properties based on these graphs. This approach is applied in this paper to predict the thermodynamic properties of a set of 5 new molecules. Le procédé d’absorption aux amines est considéré comme la technologie la plus efficace pour limiter les rejets de CO2 dans le cadre du captage en postcombustion puis du stockage du CO2. Cependant, l’optimisation des propriétés du solvant nécessite d’évaluer un grand nombre de candidats potentiels et donc de collecter une quantité importante de propriétés expérimentales. Dans ce contexte, l’utilisation de méthodes de mod

  15. Formation of biogenic amines and vitamin K contents in the Norwegian autochthonous cheese Gamalost during ripening

    OpenAIRE

    Qureshi, Tahir; Vermeer, Cees; Vegarud, Gerd; Abrahamsen, Roger; Skeie, Siv

    2013-01-01

    Gamalost, a Norwegian mould (Mucor mucedo) ripened autochthonous cheese, is a potential functional food due to a high content of peptides that might reduce hypertension, however it has a high content of free amino acids which may be precursors for biogenic amines. This study aimed to investigate if Gamalost might have further health benefits or risks by determination of the formation of vitamin K and biogenic amines. The development of biogenic amines and vitamin K was analysed during ripenin...

  16. Amine Functionalized Porous Network

    KAUST Repository

    Eddaoudi, Mohamed

    2015-05-28

    Amine groups can be introduced in porous materials by a direct (one pot) or post-synthetic modification (PSM) process on aldehyde groups, and the resulting porous materials have increased gas affinity.

  17. Unusual Fragmentation of Peptide and Protein in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    Mitsuo Takayama

    2001-01-01

    Unusual amine - bond fragmentation on the peptide/protein backbone has been reported using matrix - assisted laser desorption/ionization time - of- flight mass spectrometry (MALDI - TOFMS)The amine - bond cleavage occurred without metastable decay, while the peptide - bond cleavage occurred with metastable decay of peptide ions in a drift region of TOF mass analyzer. It was presumed that the amine - bond cleavage occurred as a non - ergodic process independent of the ionization under MALDI conditions.

  18. Organic chemistry. Strain-release amination.

    Science.gov (United States)

    Gianatassio, Ryan; Lopchuk, Justin M; Wang, Jie; Pan, Chung-Mao; Malins, Lara R; Prieto, Liher; Brandt, Thomas A; Collins, Michael R; Gallego, Gary M; Sach, Neal W; Spangler, Jillian E; Zhu, Huichin; Zhu, Jinjiang; Baran, Phil S

    2016-01-15

    To optimize drug candidates, modern medicinal chemists are increasingly turning to an unconventional structural motif: small, strained ring systems. However, the difficulty of introducing substituents such as bicyclo[1.1.1]pentanes, azetidines, or cyclobutanes often outweighs the challenge of synthesizing the parent scaffold itself. Thus, there is an urgent need for general methods to rapidly and directly append such groups onto core scaffolds. Here we report a general strategy to harness the embedded potential energy of effectively spring-loaded C-C and C-N bonds with the most oft-encountered nucleophiles in pharmaceutical chemistry, amines. Strain-release amination can diversify a range of substrates with a multitude of desirable bioisosteres at both the early and late stages of a synthesis. The technique has also been applied to peptide labeling and bioconjugation.

  19. Du fratinoj

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    Vivis iam du fratinoj-Aje kaj Fatme.Iliapatrino estis duon-patrino por Fatme kaj in neamis.Foje i diris al sia edzo:-Faru kion ajn,sed mi ne plu volas vidiFatme.La sekvan tagon la patro forkondukis la

  20. Synthesis of peptide thioacids at neutral pH using bis(2-sulfanylethyl)amido peptide precursors.

    Science.gov (United States)

    Pira, Silvain L; Boll, Emmanuelle; Melnyk, Oleg

    2013-10-18

    Reaction of bis(2-sulfanylethyl)amido (SEA) peptides with triisopropylsilylthiol in water at neutral pH yields peptide thiocarboxylates. An alkylthioester derived from β-alanine was used to trap the released bis(2-sulfanylethyl)amine and displace the equilibrium toward the peptide thiocarboxylate. PMID:24073852

  1. Biogenic amines in dairy products

    OpenAIRE

    Linares, Daniel M.; Martín, M. Cruz; Ladero Losada, Víctor Manuel; Álvarez González, Miguel Ángel; Fernández García, María

    2011-01-01

    Biogenic amines (BA) are organic, basic, nitrogenous compounds with biological activity, mainly formed by the decarboxylation of amino acids. BA are present in a wide range of foods, including dairy products, and can accumulate in high concentrations. In some cheeses more than 1000 mg of BA have been detected per kilogram of cheese. The consumption of food containing large amounts of these amines can have toxicological consequences. Although there is no specific legislation regarding the BA c...

  2. Stereocontrolled Synthesis of Methyl Silanediol Peptide Mimics

    DEFF Research Database (Denmark)

    Nielsen, Lone; Lindsay, Karl; Faber, Jesper;

    2007-01-01

    methanolic HCl and the resulting amine extended into peptide chains accordingly. The diphenylsilyl moiety is a resilient protecting group for the corresponding silanediol, which can be unmasked via treatment with TfOH, followed by aqueous hydrolysis. The crude silanediol may be isolated and purified as its...

  3. Amine Swingbed Payload Project Management

    Science.gov (United States)

    Walsch, Mary; Curley, Su

    2013-01-01

    The International Space Station (ISS) has been designed as a laboratory for demonstrating technologies in a microgravity environment, benefitting exploration programs by reducing the overall risk of implementing such technologies in new spacecraft. At the beginning of fiscal year 2010, the ISS program manager requested that the amine-based, pressure-swing carbon dioxide and humidity absorption technology (designed by Hamilton Sundstrand, baselined for the Orion Multi-Purpose Crew Vehicle, and tested at the Johnson Space Center in relevant environments, including with humans, since 2005) be developed into a payload for ISS Utilization. In addition to evaluating the amine technology in a flight environment before the first launch of the Orion vehicle, the ISS program wanted to determine the capability of the amine technology to remove carbon dioxide from the ISS cabin environment at the metabolic rate of the full 6 ]person crew. Because the amine technology vents the absorbed carbon dioxide and water vapor to space vacuum (open loop), additional hardware needed to be developed to minimize the amount of air and water resources lost overboard. Additionally, the payload system would be launched on two separate Space Shuttle flights, with the heart of the payload-the swingbed unit itself-launching a full year before the remainder of the payload. This paper discusses the project management and challenges of developing the amine swingbed payload in order to accomplish the technology objectives of both the open -loop Orion application as well as the closed-loop ISS application.

  4. Bacterial degradation of monocyclic aromatic amines

    Directory of Open Access Journals (Sweden)

    Pankaj Kumar Arora

    2015-08-01

    Full Text Available Aromatic amines are an important group of industrial chemicals, which are widely used for manufacturing of dyes, pesticides, drugs, pigments, and other industrial products. These compounds have been considered highly toxic to human beings due to their carcinogenic nature. Three groups of aromatic amines have been recognized: monocyclic, polycyclic and heterocyclic aromatic amines. Bacterial degradation of several monocyclic aromatic compounds has been studied in a variety of bacteria, which utilizes monocyclic aromatic amines as their sole source of carbon and energy. Several degradation pathways have been proposed and the related enzymes and genes have also been characterized. Many reviews have been reviewed toxicity of monocyclic aromatic amines; however, there is lack of review on biodegradation of monocyclic aromatic amines. The aim of this review is to summarize bacterial degradation of monocyclic aromatic amines. This review will increase our current understanding of biochemical and molecular basis of bacterial degradation of monocyclic aromatic amines.

  5. Les mots du secret

    OpenAIRE

    Delage, Agnès

    2016-01-01

    Cet article analyse les reconfigurations sémantiques du lexique et des mots du secret en usage dans l’Europe du Sud entre le Moyen Âge et l’époque moderne. En partant des approches récentes du secret et de la dissimulation dans un contexte contemporain de revendication d’un « droit au secret », nous analysons comment l’historiographie actuelle des XVIe et XVIIe siècles aborde une histoire longue des régimes de positivité du secret en Europe. En partant de l’époque pré-moderne, nous étudions l...

  6. Restaurant du Rivage, Vevey

    OpenAIRE

    Basini, Sari Bianca; Glocki, Ryszard Nikodem

    2015-01-01

    Après cinquante ans de mutilations, d'abandon et de spéculations économiques, le complexe du château de l'Aile et de la salle du Castillo à Vevey doit redéfinir son rôle public par rapport à la place du Marché et au Jardin du Rivage. S'appuyant sur la mémoire historique en ajoutant une unité à l'ensemble, nous créons un îlot regroupant des fonctions publiques. Il dessert ainsi l'espace ouvert environnant en articulant la relation entre le jardin et la place. L'élargissement de la promenade du...

  7. Du taureau au dindon

    OpenAIRE

    Saumade, Frédéric

    2005-01-01

    L’importation du cheval et du taureau en Méso-Amérique et la diffusion corrélative de la corrida comme représentation ostentatoire du pouvoir espagnol ont eu pour conséquence la transformation de la structure originelle du jeu taurino-équestre. Le vecteur de ce système est un paradoxe technologique : la monte du taureau, soit une inversion de l’équitation dont l’initiative historique revient aux péons indigènes exclus de la pratique équestre à l’époque coloniale. À partir d’une approche ethno...

  8. Atmospheric amines - Part I. A review

    Science.gov (United States)

    Ge, Xinlei; Wexler, Anthony S.; Clegg, Simon L.

    2011-01-01

    Amines are emitted by a wide range of sources and are ubiquitous atmospheric organic bases. Approximately 150 amines and about 30 amino acids have been identified in the atmosphere. We review the present knowledge of atmospherically relevant amines with respect to their sources, fluxes, and dynamics including gas-phase reactions, gas-to-particle conversion and deposition. The health effects of aliphatic and aromatic amines are briefly summarized as well as the atmospheric occurrence and reactivity of amino acids and urea.

  9. Advances in heterocyclic ketene aminals

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Recent developments in the study of the reactions of heterocyclic ketene aminals are reviewed with the emphases on regioselective alkylation, acylation and glycosylation reactions, and on the aza-ene reactions with α,β-unsaturated compounds, azo and carbonyl compounds. Reactions with 1,3-dipoles and other reagents to synthesize fused heterocycles are also discussed.

  10. Ecologie du phytoplancton du lac Kivu

    Directory of Open Access Journals (Sweden)

    Sarmento, H.

    2008-01-01

    Full Text Available Speciation within the African Coffee Pathogen. Cet article analyse s'il est avantageux d'utiliser le compost au lieu de l'engrais minéral pour produire la laitue dans la zone urbaine et péri-urbaine de Yaoundé. Les résultats de terrain montrent l'obtention de rendements et profits plus élevés lorsqu'on utilise le compost. Les résultats de la fonction de production Cobb-Douglas prouvent que l'utilisation du compost est statistiquement significative pour expliquer la variation de rendement de la laitue et que le compost est l'intrant le plus productif. D'autres résultats montrent que le compost fournit la matière organique utile au sol et que les besoins d'irrigation en eau de la culture sont réduits grâce à l'utilisation du compost. Par conséquent, malgré le fait que l'application du compost demande une main-d'oeuvre beaucoup plus élevée, son utilisation est généralement bénéfique pour les agriculteurs vivant aux alentours de Yaoundé. Les programmes de vulgarisation de cet intrant pour encourager son adoption devraient donc figurer parmi les points prioritaires dans la politique agricole du gouvernement camerounais.

  11. Signification politique du climat

    OpenAIRE

    Lamizet, Bernard

    2013-01-01

    Le climat fonde une approche politique particulière de l’espace et du temps, en suscitant des représentations particulières se situant dans l’histoire, la mémoire, la prévision et le domaine du développement durable. Le climat représente aussi une forme particulière de limitation des pouvoirs et de représentation de la contrainte dans le discours politique et dans les médias, jusqu’à figurer une forme de violence. Enfin, les significations du climat se situent dans l’inconscient. The notio...

  12. Types du Caucase

    OpenAIRE

    Makhacheva, Taus

    2015-01-01

    Tiré du site Internet de Onestar Press: "Types du Caucase - antique postcard collection/ 2013 to the present time. Coming from the personal archive of the artist, these postcards date back to the 19th century and can be considered representative for the "popularized ethnography" of the Russian Empire. Their primitive typology follows the classical rules of exotization : they depict "pittoresque" groups from various tribes, families or nationalities, or representatives of diverse professions. ...

  13. Les outils du CERN

    CERN Multimedia

    1999-01-01

    C'est le plus grand centre mondial de recherche en physique des particules. Les outils du Laboratoire, accélérateurs et détecteurs de particules, figurent parmi les instruments scientifiques les plus complexes au monde. Des prix Nobels ont d'ailleurs été attribués aux physiciens du CERN pour leurs développements.

  14. Analysis of heterocyclic aromatic amines.

    Science.gov (United States)

    Murkovic, M

    2007-09-01

    Heterocyclic aromatic amines are formed in protein and amino acid-rich foods at temperatures above 150 degrees C. Of more than twenty heterocyclic aromatic amines identified ten have been shown to have carcinogenic potential. As nutritional hazards, their reliable determination in prepared food, their uptake and elimination in living organisms, including humans, and assessment of associated risks are important food-safety issues. The concentration in foods is normally in the low ng g(-1) range, which poses a challenge to the analytical chemist. Because of the complex nature of food matrixes, clean-up and enrichment of the extracts are also complex, usually involving both cation-exchange (propylsulfonic acid silica gel, PRS) and reversed-phase purification. The application of novel solid-phase extraction cartridges with a wettable apolar phase combined with cation-exchange characteristics simplified this process--both the polar and apolar heterocyclic aromatic amines were recovered in one fraction. Copper phthalocyanine trisulfonate bonded to cotton ("blue cotton") or rayon, and molecular imprinted polymers have also been successfully used for one-step sample clean-up. For analysis of the heterocyclic aromatic amines, liquid chromatography with base-deactivated reversed-phase columns has been used, and, recently, semi-micro and capillary columns have been introduced. The photometric, fluorimetric, or electrochemical detectors used previously have been replaced by mass spectrometers. Increased specificity and sub-ppb sensitivities have been achieved by the use of the selected-reaction-monitoring mode of detection of advanced MS instrumentation, for example the triple quadrupole and Q-TOF instrument combination. Gas chromatography, also with mass-selective detection, has been used for specific applications; the extra derivatization step needed for volatilization has been balanced by the higher chromatographic resolution. PMID:17546447

  15. Film-forming amines in shell boilers

    Energy Technology Data Exchange (ETDEWEB)

    Topp, Holger [Astrium RST Rostock GmbH, Rostock (Germany); Hater, Wolfgang [BK Giulini, Ludwigshafen (Germany); BKG Water Solutions, Ludwigshafen (Germany); Bache, Andre de [BK Giulini, Ludwigshafen (Germany); Kolk, Christian zum [BKG Water Solutions, Ludwigshafen (Germany)

    2012-01-15

    Studies were conducted with the aim of providing answers to important questions concerning the use of film-forming amines in steam generators. Tests were carried out in test steam generators under controlled conditions to study the three following application areas: the influence of film-forming amines on boiling behavior and heat transfer, the influence of film-forming amines on oxidic protective film formation, and the influence of film-forming amines on critical operating conditions. In the experiments water treatment with trisodium phosphate (which is normally used with shell boilers) was compared with treatment with film-forming amines. In all three areas the treatment with film-forming amines achieved comparable or better results than the treatment with trisodium phosphate. (orig.)

  16. Biogenic amines in seafood: a review.

    Science.gov (United States)

    Biji, K B; Ravishankar, C N; Venkateswarlu, R; Mohan, C O; Gopal, T K Srinivasa

    2016-05-01

    The biogenic amines are low molecular weight organic bases present normally in the body with biological activity influencing important physiological functions. The physiological functions of these molecules are achieved by very low concentrations in the tissues. However, significantly high amounts of biogenic amines are produced during processing and storage of seafood as a result of microbial contamination and inadequate storage conditions. Microorganisms having decarboxylase enzyme activity convert amino acids to their respective biogenic amines. Biogenic amines in seafood have been implicated as a major causative agent of food borne illness, where intoxication results from the ingestion of foods containing higher amount of biogenic amines. Hence its identification, quantitation and awareness of this food borne toxin are important in relation to food safety and spoilage. The aim of this paper is to review the basic concepts of seafood quality and safety in relation to biogenic amines along with its control measures and future areas for research. PMID:27407186

  17. New dendrimer - Peptide host - Guest complexes: Towards dendrimers as peptide carriers

    DEFF Research Database (Denmark)

    Boas, Ulrik; Sontjens, S.H.M.; Jensen, Knud Jørgen;

    2002-01-01

    the NH- and CO-stretch signals of the peptide amide moieties shift towards lower wave-numbers upon complexation with the dendrimer. Spatial analysis of the complexes with NOESY spectroscopy generally shows close proximity of the N-terminal Boc group of the peptide to the peripheral adamantyl groups...... between the dendrimer outer shell tertiary amines and the C-terminal carboxylic acid of the peptide, and also through host-urea to peptide-amide hydrogen bonding. The hydrogen-bonding nature of the peptide dendrimer interactions was further confirmed by using Fourier transform IR spectroscopy, for which...... on the dendrimer host. The influence of side-chain motif on interactions with the host is analyzed by using seven different N-Boc-protected tripeptides as guests for the dendrimer, Downfield shifts of up to 1.3 ppm were observed for the guest amide NH-proton signals. These shifts decrease with increasing...

  18. BIOGENIC AMINES CONTENT IN DIFFERENT WINE SAMPLES

    OpenAIRE

    Attila Kántor; Miroslava Kačániová; Vendula Pachlová

    2015-01-01

    Twenty-five samples of different Slovak wines before and after filtration were analysed in order to determine the content of eight biogenic amines (tryptamine, phenylalanine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine). The method involves extraction of biogenic amines from wine samples with used dansyl chloride. Ultra-high performance liquid chromatography (UHPLC) was used for determination of biogenic amines equipped with a Rapid Resolution High Definition (RRHD), ...

  19. A ruthenium racemisation catalyst for the synthesis of primary amines from secondary amines.

    Science.gov (United States)

    Pingen, Dennis; Altıntaş, Çiğdem; Rudolf Schaller, Max; Vogt, Dieter

    2016-08-01

    A Ru-based half sandwich complex used in amine and alcohol racemization reactions was found to be active in the splitting of secondary amines to primary amines using NH3. Conversions up to 80% along with very high selectivities were achieved. However, after about 80% conversion the catalyst lost activity. Similar to Shvo's catalyst, the complex might deactivate under the influence of ammonia. It was revealed that not NH3 but mainly the primary amine is responsible for the deactivation. PMID:27321431

  20. Droit du travail

    OpenAIRE

    De Quenaudon, René

    2016-01-01

    L’association des deux notions, celle de droit du travail et celle de RSE (responsabilité sociale des entreprises), relève du défi, tout au moins dans le contexte juridique français. C’est un peu comme si l’on prétendait pouvoir mélanger l’eau et le feu. Pourquoi ? Parce que classiquement le droit du travail est présenté comme un ensemble de règles assorties de la contrainte étatique dans les relations entre employeurs et salariés alors que la RSE est présentée comme un ensemble d’engagements...

  1. Michael addition of thiols, carbon nucleophiles and amines to dehydroamino acid and dehydropeptide derivatives

    OpenAIRE

    Ferreira, Paula M.T.; Maia, Hernâni L. S.; Monteiro, Luís S.; Sacramento, Joana

    2001-01-01

    Michael additions of nitrogen heterocycles, thiols, carbon nucleophiles and amines to dehydroalanine derivatives, including a glycyldehydroalanine peptide, were performed in fair to good yields. Dehydroaminobutyric acid derivatives reacted only with the stronger nucleophiles but in considerably lower yields and often no reaction was observed with the corresponding dehydrophenylalanine derivatives. When a tosyl group was bonded to the nitrogen atom of the dehydroamino acid, in some cases the a...

  2. Une science du tourisme ?

    OpenAIRE

    Airey, David; Bédard, François; Ceriani-Sebregondi, Giorgia; Chapuis, Amandine; Dewailly, Jean-Michel; Drouin, Martin; Gay, Jean-Christophe; Hillali, Mimoun; Hoerner, Jean-Michel; Jolin, Louis; Kadri, Boualem; Rémy KNAFOU; Leman, Edward; Novakowski, Nick; Priskin, Julianna

    2009-01-01

    Avec ce numéro thématique, Téoros souhaite promouvoir un débat organisé dans une perspective internationale et pluridisciplinaire. L’objectif de ce numéro est de s’interroger à nouveau sur l’identité scientifique du tourisme, en tentant de mettre en évidence les handicaps épistémologiques et méthodologiques, de comprendre les raisons qui freinent la construction d’un consensus au sein de la communauté scientifique, d’identifier les conditions nécessaires à l’émergence d’une science du tourism...

  3. Amine degradation and associated problems in the gas treating unit

    Energy Technology Data Exchange (ETDEWEB)

    Baumeister, E.R. [Dow Chemical Co. (United States); Souza, R.C.O [Dow Brasil S.A., Sao Paulo, SP (Brazil)

    2008-07-01

    Amine degradation and associated problems in the amine gas treating unit cannot be completely avoided or eliminated. They can be cost effectively mitigated using a thorough amine management program. Economic and performance benefits realized from an amine management program include several improvements and performance index. Better yields, lower costs, and fewer environmental concerns are some of the additional advantages of using a comprehensive amine management system to help run the amine gas treating unit. (author)

  4. Speaking Personally--with Amin Qazi

    Science.gov (United States)

    Ragan, Lawrence

    2016-01-01

    This article provides an interview with Amin Quazi, the founding chief executive officer of Unizin, a university-owned consortium focused on the emerging digital teaching and learning ecosystem. Amin has a bachelor's degree in chemical engineering from The University of Iowa and a master's degree in business administration from the Carlson School…

  5. Intolerance to dietary biogenic amines: A review

    NARCIS (Netherlands)

    Jansen, S.C.; Dusseldorp, M. van; Bottema, K.C.; Dubois, A.E.J.

    2003-01-01

    Objective: To evaluate the scientific evidence for purported intolerance to dietary biogenic amines. Data Sources: MEDLINE was searched for articles in the English language published between January 1966 and August 2001. The keyword biogenic amin* was combined with hypersens*, allerg*, intoler*, and

  6. Discovery of Spiro[cyclohexane-dihydropyrano[3,4-b]indole]-amines as Potent NOP and Opioid Receptor Agonists.

    Science.gov (United States)

    Schunk, Stefan; Linz, Klaus; Frormann, Sven; Hinze, Claudia; Oberbörsch, Stefan; Sundermann, Bernd; Zemolka, Saskia; Englberger, Werner; Germann, Tieno; Christoph, Thomas; Kögel, Babette-Y; Schröder, Wolfgang; Harlfinger, Stephanie; Saunders, Derek; Kless, Achim; Schick, Hans; Sonnenschein, Helmut

    2014-08-14

    We report the discovery of spiro[cyclohexane-pyrano[3,4-b]indole]-amines, as functional nociceptin/orphanin FQ peptide (NOP) and opioid receptor agonists with strong efficacy in preclinical models of acute and neuropathic pain. Utilizing 4-(dimethylamino)-4-phenylcyclo-hexanone 1 and tryptophol in an oxa-Pictet-Spengler reaction led to the formation of spiroether 2, representing a novel NOP and opioid peptide receptor agonistic chemotype. This finding initially stems from the systematic derivatization of 1, which resulted in alcohols 3-5, ethers 6 and 7, amines 8-10, 22-24, and 26-28, amides 11 and 25, and urea 12, many with low nanomolar binding affinities at the NOP and mu opioid peptide (MOP) receptors. PMID:25147602

  7. Historique du Web

    CERN Multimedia

    TV8 Mont-Blanc

    1995-01-01

    Documentaire court qui retrace l'implication de différentes personalités à la création du Web. Entrevues avec Robert Cailliau, Chris Llewellyn-Smith, David Williams, Tim Berners-Lee, Mike Sendall, Brian Carpenter.

  8. Thermodynamics of mixtures containing amines

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Juan Antonio [G.E.T.E.F. Dpto Termodinamica y Fisica Aplicada, Facultad de Ciencias, Universidad de Valladolid, Valladolid 47071 (Spain)], E-mail: jagl@termo.uva.es; Mozo, Ismael; Garcia de la Fuente, Isaias; Cobos, Jose Carlos [G.E.T.E.F. Dpto Termodinamica y Fisica Aplicada, Facultad de Ciencias, Universidad de Valladolid, Valladolid 47071 (Spain); Riesco, Nicolas [Department of Chemical Engineering, Loughborough University, Loughborough, LE113TU Leicestershire (United Kingdom)

    2008-01-30

    Mixtures with dimethyl or trimethylpyridines and alkane, aromatic compound or 1-alkanol have been examined using different theories: DISQUAC, Flory, the concentration-concentration structure factor, S{sub CC}(0), or the Kirkwood-Buff formalism. DISQUAC represents fairly well the available experimental data, and improves theoretical calculations from Dortmund UNIFAC. Two important effects have been investigated: (i) the effect of increasing the number of methyl groups attached to the aromatic ring of the amine; (ii) the effect of modifying the position of the methyl groups in this ring. The molar excess enthalpy, H{sup E}, and the molar excess volume, V{sup E}, decrease in systems with alkane or methanol as follows: pyridine > 3-methylpyridine > 3,5-dimethylpyridine and pyridine > 2-methylpyridine > 2,4-dimethylpyridine > 2,4,6-trimethylpyridine, which has been attributed to a weakening of the amine-amine interactions in the same sequences. This is in agreement with the relative variation of the effective dipole moment, {mu}-bar, and of the differences between the boiling temperature of a pyridine base and that of the homomorphic alkane. For heptane solutions, the observed H{sup E} variation, H{sup E} (3,5-dimethylpyridine) > H{sup E} (2,4-dimethylpyridine) > H{sup E} (2,6-dimethylpyridine), is explained similarly. Calculations on the basis of the Flory model confirm that orientational effects become weaker in systems with alkane in the order: pyridine > methylpyridine > dimethylpyridine > trimethylpyridine. S{sub CC}(0) calculations show that steric effects increase with the number of CH{sub 3}- groups in the pyridine base, and that the steric effects exerted by methyl groups in positions 2 and 6 are higher than when they are placed in positions 3 and 5. The hydrogen bond energy in methanol mixtures is independent of the pyridine base, and it is estimated to be -35.2 kJ mol{sup -1}. Heterocoordination in these solutions is due in part to size effects. Their

  9. Tyrosine-containing peptides are precursors of tyramine produced by Lactobacillus plantarum strain IR BL0076 isolated from wine

    Directory of Open Access Journals (Sweden)

    Bonnin-Jusserand Maryse

    2012-09-01

    Full Text Available Abstract Background Biogenic amines are molecules with allergenic properties. They are found in fermented products and are synthesized by lactic acid bacteria through the decarboxylation of amino acids present in the food matrix. The concentration of biogenic amines in fermented foodstuffs is influenced by many environmental factors, and in particular, biogenic amine accumulation depends on the quantity of available precursors. Enological practices which lead to an enrichment in nitrogen compounds therefore favor biogenic amine production in wine. Free amino acids are the only known precursors for the synthesis of biogenic amines, and no direct link has previously been demonstrated between the use of peptides by lactic acid bacteria and biogenic amine synthesis. Results Here we demonstrate for the first time that a Lactobacillus plantarum strain isolated from a red wine can produce the biogenic amine tyramine from peptides containing tyrosine. In our conditions, most of the tyramine was produced during the late exponential growth phase, coinciding with the expression of the tyrDC and tyrP genes. The DNA sequences of tyrDC and tyrP in this strain share 98% identity with those in Lactobacillus brevis consistent with horizontal gene transfer from L. brevis to L. plantarum. Conclusion Peptides amino acids are precursors of biogenic amines for Lactobacillus plantarum strain IR BL0076.

  10. Synthesis and In Vitro Testing of New Potent Polyacridine-Melittin Gene Delivery Peptides

    OpenAIRE

    Baumhover, Nicholas J.; Anderson, Kevin; Fernandez, Christian A.; Rice, Kevin G.

    2010-01-01

    The combination of a polyacridine peptide modified with a melittin fusogenic peptide results in a potent gene transfer agent. Polyacridine peptides of the general formula (Acr-X)n-Cys were prepared by solid phase peptide synthesis, where Acr is Lys modified on its ε-amine with acridine, X is Arg, Leu or Lys and n is 2, 3 or 4 repeats. The Cys residue was modified by either a maleimide-melittin or a thiolpyridine-Cys-melittin fusogenic peptide resulting in reducible or non-reducible polyacridi...

  11. Quantifying amine permeation sources with acid neutralization: calibrations and amines measured in coastal and continental atmospheres

    Directory of Open Access Journals (Sweden)

    N. A. Freshour

    2014-04-01

    Full Text Available An acid titration method for quantifying amine permeation rates was used to calibrate an Ambient pressure Proton transfer Mass Spectrometer (AmPMS that monitors ambient amine compounds. The method involves capturing amines entrained in a N2 flow by bubbling it through an acidified solution (~ 10−5 M HCl, and the amines are quantified via changes in solution pH with time. Home-made permeation tubes had permeation rates (typically tens of pmol s−1 that depended on the type of amine and tubing and on temperature. Calibrations of AmPMS yielded sensitivities for ammonia, methyl amine, dimethyl amine, and trimethyl amine that are close to the sensitivity assuming a gas-kinetic, ion-molecule rate coefficient. The permeation tubes were also designed to deliver a reproducible amount of amine to a flow reactor where nucleation with sulfuric acid was studied. The high proton affinity compound dimethyl sulfoxide (DMSO, linked to oceanic environments, was also studied and AmPMS is highly sensitive to it. AmPMS was deployed recently in two field campaigns and mixing ratios are reported for ammonia, alkyl amines, and DMSO and correlations between these species and with particle formation events are discussed.

  12. Improved Parameterization of Amine-Carboxyate and Amine-Phosphate Interactions for Molecular Dynamics Simulations Using the CHARMM and AMBER Force Fields

    CERN Document Server

    Yoo, Jejoong

    2015-01-01

    Over the past decades, molecular dynamics (MD) simulations of biomolecules have become a mainstream biophysics technique. As the length and time scales amenable to the MD method increase, shortcomings of the empirical force fields---which have been developed and validated using relatively short simulations of small molecules---become apparent. One common artifact is aggregation of water-soluble biomolecules driven by artificially strong charge--charge interactions. Here, we report a systematic refinement of Lennard-Jones parameters (NBFIX) describing amine--carboxylate and amine--phosphate interactions, which brings MD simulations of basic peptide-mediated nucleic acids assemblies and lipid bilayer membranes in better agreement with experiment. As our refinement neither affects the existing parameterization of bonded interaction nor does it alter the solvation free energies, it improves realism of an MD simulation without introducing additional artifacts.

  13. biogenic aerosol precursors: volatile amines from agriculture

    Science.gov (United States)

    Kuhn, Uwe; Sintermann, Jörg; Spirig, Christoph; Ammann, Christof; Neftel, Albrecht

    2010-05-01

    Information on the occurrence of volatile biogenic amines in the atmosphere is marginal. This group of N-bearing organic compounds are assumed to be a small, though significant component of the atmospheric N-cycle, but are not accounted for in global assessments due to the scarceness of available data. There is increasing evidence for an important role of biogenic amines in the formation of new particulate matter, as well as for aerosol secondary growth. Volatile amines are ubiquitously formed by biodegradation of organic matter, and agriculture is assumed to dominantly contribute to their atmospheric burden. Here we show that the mixing ratios of volatile amines within livestock buildings scale about 2 orders of magnitude lower than NH3, confirming the few literature data available (e.g., Schade and Crutzen, J. Atm. Chem. 22, 319-346, 1995). Flux measurements after manure application in the field, mixing ratios in the headspace of manure storage pools, and concentrations in distilled manure all indicate major depletion of amines relative to NH3 during manure processing. We conclude that the agricultural source distribution of NH3 and amines is not similar. While for NH3 the spreading of manure in the field dominates agricultural emissions, the direct release from livestock buildings dominates the budget of volatile biogenic amines.

  14. Biogenic amines and radiosensitivity of solitary cells

    International Nuclear Information System (INIS)

    Different stability of cells to ionizing radiation is considered from a position of the ''elevated biochemical radioresistance background'' concept. Experimental evidence presented indicates an important role of endogenic amines (serotonin and histamine) possessing radioprotector properties in the cell radioresistance formation. The concept about their effect as being solely a result of circulatory hypoxia is critically discussed. The experimental results favor the existence of a ''cellular'' component, along with the ''hypoxic'' one, in the mechanism of action of biogenic amines. These compounds can affect the initial stages of peroxide oxidation of lipids, thereby favoring a less intensive oxidation induced by radiation. Biogenic amines can also exert influence on the cyclic nucleotide system

  15. Approches contemporaines du bonheur

    OpenAIRE

    Guibet-Lafaye, Caroline

    2013-01-01

    1. Les centres cérébraux du bonheur La pensée et les recherches contemporaines offrent-elles une meilleure compréhension du bonheur de l’homme ? Qu’ont-elles apporté à la réflexion sur le bonheur et à son identification ou à sa détermination ? Les sciences sociales ne se sont intéressées que tardivement à la question de la vie bonne qui a longtemps été le domaine réservé de la philosophie et de la théologie. La psychiatrie fut la première, en raison de son travail sur la dépression, à s’intér...

  16. Jouer du piano

    OpenAIRE

    Fériel Kaddour

    2011-01-01

    La réflexion s’appuie dans un premier temps sur une opposition entre deux attitudes de pianistes  à l’égard du travail à l’instrument : Gould, qui revendique une séparation d’avec le clavier pour ne privilégier que la lecture; Arrau, dont la technique au contraire vise à « faire corps » avec son piano. L’étude de ces deux démarches d’interprètes conduit à une conclusion croisée : l’abstraction gouldienne n’est rien d’autre qu’un déplacement du jeu vers d’autres instruments (ceux qui servent à...

  17. CHOEUR DU CERN

    CERN Document Server

    CHOEUR DU CERN

    2010-01-01

    Les répétitions du chœur du CERN reprendront le mercredi 15 septembre à 20.00 heures à l’amphithéâtre principal – bâtiment 500. Au programme la préparation de notre concert de Noël avec la Missa Brevis, KV115, de Léopold Mozart et de la musique de Noël d’Europe. Les personnes qui aiment chanter, notamment des sopranes et des ténors, sont les bienvenues. Pour tout contact s’adresser à : Baudouin Bleus - (tél.CERN 767 82 44) -(baudouin.bleus@cern.ch) ou Martin Gatehouse ( martin.gatehouse@wanadoo.fr) ou Jean-Paul Diss (jean-pauldiss@wanadoo.fr).  

  18. Le chant du monde

    OpenAIRE

    Roger, Philippe

    2015-01-01

    Jean Grémillon emploie la voix dans ses films de manière très différente de ses collègues. C’est l’ensemble du sonore – bruits, voix et musique – qu’il imagine de façon originale. Son traitement s’inscrit dans une vision du monde : Grémillon conçoit le cinématographe comme un biographe, une écriture de la vie. Cette vie est comprise sur deux plans : celui de l’homme et celui de l’univers. La voix selon Grémillon serait le reflet dans l’homme d’un macrocosme perçu comme énigme (le bruit) ou ré...

  19. Les Conidae du Surinam

    NARCIS (Netherlands)

    Mol, van J.-J.

    1973-01-01

    RÉSUMÉ Les prospections des navires océanographiques Snellius et Luymes au large des côtes du Surinam révèlent l'existence de sept espèces de Conidae. Parmi celles-ci une espèce, Conus guyanensis spec. nov., semble endémique à cette région. Six espèces (Conus centurio, C. austini, C. daucus, C. jasp

  20. Advance in the synthesis of aromatic amine via direct amination

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Arylamines are very common and important organic molecules. Acting as important intermediates in medical and chemical industry, they are widely used in the synthesis of pharmaceuticals, dyes, pesticides, rubber additives, isocyanates and heterocyclic compounds. The traditional methods for the synthesis of arylamines include several steps. The production of aniline, for example, includes the following steps: the production of nitro-benzene by the nitration of benzene, and the reduction of the resulting nitro-benzene by catalytic hydrogenation or other reduction ways[1-6]; or the substitution of phenol or chlorbenzene by amino groups, etc.[7-10]. There are several disadvantages for these methods: low atom utilization, strict operation condition, large amount of by-products and serious environmental pollution, which could not afford for the needs of a sustainable civilization. So there has been growing interest in the direct amination to produce arylamines recently. With the direct methods, the multi- step reaction may change into one step, the atom utilization can be evidently improved, and the by-products H2 or/and H2O are harmless to the environment.

  1. La mesure du danger

    CERN Document Server

    Manceron, Vanessa; Revet, Sandrine

    2014-01-01

    La mesure du danger permet d’explorer des dangers de nature aussi diverse que la délinquance, la pollution, l’écueil maritime, la maladie ou l’attaque sorcellaire, l’extinction d’espèces animales ou végétales, voire de la Planète tout entière. Au croisement de la sociologie, de l’anthropologie et de l’histoire, les différents articles analysent les pratiques concrètes de mesure pour tenter de comprendre ce qui se produit au cours de l’opération d’évaluation du danger sans préjuger de la nature de celui-ci. L’anthropologie a contribué à la réflexion sur l’infortune en s’intéressant aux temporalités de l’après : maladies, catastrophes, pandémies, etc. et en cherchant à rendre compte de l’expérience des victimes, de leur vie ordinaire bouleversée, de la recomposition du quotidien. Elle s’intéresse aussi aux autres types de mesures, les savoirs incorporés, qui reposent sur l’odorat, la vue ou le toucher et ceux qui ressortent d’une épistémologie « non ...

  2. Graphite Oxide and Aromatic Amines : Size Matters

    NARCIS (Netherlands)

    Spyrou, Konstantinos; Calvaresi, Matteo; Diamanti, Evmorfi A. K.; Tsoufis, Theodoros; Gournis, Dimitrios; Rudolf, Petra; Zerbetto, Francesco

    2015-01-01

    Experimental and theoretical studies are performed in order to illuminate, for first time, the intercalation mechanism of polycyclic aromatic molecules into graphite oxide. Two representative molecules of this family, aniline and naphthalene amine are investigated. After intercalation, aniline molec

  3. Aromatic amines sources, environmental impact and remediation

    OpenAIRE

    Pereira, Luciana; Mondal, P. K.; Alves, M. M.

    2015-01-01

    Aromatic amines are widely used industrial chemicals as their major sources in the environment include several chemical industry sectors such as oil refining, synthetic polymers, dyes, adhesives, rubbers, perfume, pharmaceuticals, pesticides and explosives. They result also from diesel exhaust, combustion of wood chips and rubber and tobacco smoke. Some types of aromatic amines are generated during cooking, special grilled meat and fish, as well. The intensive use and production of these comp...

  4. Surface amination of poly(acrylonitrile)

    OpenAIRE

    Hartwig, Andreas; Mulder, Marcel; Smolders, Cees A.

    1994-01-01

    The surface amination of poly (acrylonitrile) by ammonia plasma treatment has been studied. Furthermore, two other surface modification techniques have been investigated, the plasma chemical decomposition of an amino group containing chemical (tris-(2-aminoethyl)amine) onto the polymer surface and the surface reduction by lithium aluminium hydride. The three different methods are compared with respect to the adhesion improvement of the coatings onto the modified surfaces.

  5. Biogenic amines in raw and processed seafood

    OpenAIRE

    Pierina eVisciano; Maria eSchirone; Rosanna eTofalo; Giovanna eSuzzi

    2012-01-01

    The presence of biogenic amines in raw and processed seafood, associated with either time/temperature conditions or food technologies is discussed in the present paper from a safety and prevention point of view. In particular, storage temperature, handling practices, presence of microbial populations with decarboxylase activity and availability of free amino acids are considered the most important factors affecting the production of biogenic amines in raw seafood. On the other hand, some foo...

  6. Enhancing amine terminals in an amine-deprived collagen matrix.

    LENUS (Irish Health Repository)

    Tiong, William H C

    2008-10-21

    Collagen, though widely used as a core biomaterial in many clinical applications, is often limited by its rapid degradability which prevents full exploitation of its potential in vivo. Polyamidoamine (PAMAM) dendrimer, a highly branched macromolecule, possesses versatile multiterminal amine surface groups that enable them to be tethered to collagen molecules and enhance their potential. In this study, we hypothesized that incorporation of PAMAM dendrimer in a collagen matrix through cross-linking will result in a durable, cross-linked collagen biomaterial with free -NH 2 groups available for further multi-biomolecular tethering. The aim of this study was to assess the physicochemical properties of a G1 PAMAM cross-linked collagen matrix and its cellular sustainability in vitro. Different amounts of G1 PAMAM dendrimer (5 or 10 mg) were integrated into bovine-derived collagen matrices through a cross-linking process, mediated by 5 or 25 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) in 5 mM N-hydroxysuccinimide (NHS) and 50 mM 2-morpholinoethane sulfonic acid buffer at pH 5.5. The physicochemical properties of resultant matrices were investigated with scanning electron microscopy (SEM), collagenase degradation assay, differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectra, and ninhydrin assay. Cellular sustainability of the matrices was assessed with Alamar Blue assay and SEM. There was no significant difference in cellular behavior between the treated and nontreated groups. However, the benefit of incorporating PAMAM in the cross-linking reaction was limited when higher concentrations of either agent were used. These results confirm the hypothesis that PAMAM dendrimer can be incorporated in the collagen cross-linking process in order to modulate the properties of the resulting cross-linked collagen biomaterial with free -NH 2 groups available for multi-biomolecular tethering.

  7. Les pirogues du Maroni

    Directory of Open Access Journals (Sweden)

    Sophie François

    2012-04-01

    Full Text Available Sur le Maroni, fleuve frontière entre la Guyane française et le Surinam, les pirogues amarrées le long des berges se comptent par centaines. Elles représentent le seul moyen de transport pour les milliers de personnes vivant sur les rives du Maroni. Les différentes formes de pirogues sont révélatrices de la diversité géographique du Maroni et de la diversité culturelle des populations. Les Amérindiens du littoral naviguent uniquement dans l’estuaire du fleuve. Ils construisent des pirogues larges, hautes et terminées à la poupe par une haute étrave qui fend les vagues. Ces pirogues sont construites à partir d’une coque monoxyle expansée et rehaussée d’un ou de deux bordages. En chauffant la grume évidée, le bois acquiert une certaine plasticité qui permet d’écarter les flancs et d’obtenir une coque très large à partir d’un arbre de faible diamètre. Les Bushinenge, populations d’origine africaine qui ont déserté les grandes plantations de la Guyane hollandaise dès le début du XVIIe siècle et se sont installées sur les deux rives du Maroni, ont développé des formes de pirogues adaptées au passage des sauts. Leurs pirogues, construites également en expansant la coque au feu, sont étroites et longues et possèdent des extrémités curvilignes qui dépassent largement au–dessus du bordage. Les longues pirogues à moteur comme les canots–pagaies sont ornés de motifs d’entrelacs appelés tembé ; et de décors d’inspiration contemporaine. L’inventaire des pirogues du Maroni a mis en évidence la richesse et la vitalité du patrimoine nautique de cette région au début du XXIe siècle, tant au niveau des formes des embarcations que des décors et des pratiques de navigation.On the Maroni river, natural border between French Guyana and the Surinam, canoes tied to the river banks can be counted by the hundreds. They are the only mean of transportation for thousands of people living along the

  8. L'Origine du Monde

    CERN Multimedia

    CNET & Ecole Polytechnique Paris; Vincent Ferreira

    1996-01-01

    Création, théologie, science, découverte, recherche, religion, condition humaine.Avec Maurice Jacob physicien, Hubert Curien Président du Conseil du CERN, Michael Doser physicien, Frère Emile Communauté de Taizé.

  9. Accurate determination of aldehydes in amine catalysts or amines by 2,4-dinitrophenylhydrazine derivatization.

    Science.gov (United States)

    Barman, Bhajendra N

    2014-01-31

    Carbonyl compounds, specifically aldehydes, present in amine catalysts or amines are determined by reversed-phase liquid chromatography using ultraviolet detection of their corresponding 2,4-dinitrophenylhydrazones. The primary focus has been to establish optimum conditions for determining aldehydes accurately because these add exposure concerns when the amine catalysts are used to manufacture polyurethane products. Concentrations of aldehydes determined by this method are found to vary with the pH of the aqueous amine solution and the derivatization time, the latter being problematic when the derivatization reaction proceeds slowly and not to completion in neutral and basic media. Accurate determination of aldehydes in amines through derivatization can be carried out at an effective solution pH of about 2 and with derivatization time of 20min. Hydrochloric acid has been used for neutralization of an amine. For complete derivatization, it is essential to protonate all nitrogen atoms in the amine. An approach for the determination of an adequate amount of acid needed for complete derivatization has been described. Several 0.2M buffer solutions varying in pH from 4 to 8 have also been used to make amine solutions for carrying out derivatization of aldehydes. These solutions have effective pHs of 10 or higher and provide much lower aldehyde concentrations compared to their true values. Mechanisms for the formation of 2,4-dinitrophenylhydrazones in both acidic and basic media are discussed. PMID:24411140

  10. Reductive Amination of Aldehydes and Ketones with Primary Amines by Using Lithium Amidoborane as Reducing Reagent

    Institute of Scientific and Technical Information of China (English)

    徐维亮; 郑学丽; 吴国涛; 陈萍

    2012-01-01

    A variety of secondary amines were obtained in high isolated yields in the reductive amination of aldehydes and ketones by using lithium amidoborane as reducing agent. Compared to ammonia borane, lithium amidoborane has higher reducibility, and thus, exhibits faster reaction rate.

  11. Discours du figural

    OpenAIRE

    Sarikartal, Eminé

    2008-01-01

    Autour de la question du cheminement de l’art et de son interaction avec la technique, après une lecture de la partie prologue de La fable cinématographique de Jacques Rancière suivant le propre fil de réflexion de l’auteur, ce texte propose la rencontre de cette lecture avec la pensée de Jean-François Lyotard telle qu’il l’expose dans Discours, Figure.

  12. Solid-Phase Synthesis of Amine/Carboxyl Substituted Prolines and Proline Homologues: Scope and Limitations.

    Science.gov (United States)

    Zhou, Ziniu; Scott, William L; O'Donnell, Martin J

    2016-03-15

    A solid-phase procedure is used to synthesize racemic peptidomimetics based on the fundamental peptide unit. The peptidomimetics are constructed around proline or proline homologues variably substituted at the amine and carbonyl sites. The procedure expands the diversity of substituted peptidomimetic molecules available to the Distributed Drug Discovery (D3) project. Using a BAL-based solid-phase synthetic sequence the proline or proline homologue subunit is both constructed and incorporated into the peptidomimetic by an α-alkylation, hydrolysis and intramolecular cyclization sequence. Further transformations on solid-phase provide access to a variety of piperazine derivatives representing a class of molecules known to exhibit central nervous system activity. The procedure works well with proline cores, but with larger six- and seven-membered ring homologues the nature of the carboxylic acid acylating the cyclic amine can lead to side reactions and result in poor overall yields.

  13. Synthesis of Bis(hydroxylmethylfurfuryl)amine Monomers from 5-Hydroxymethylfurfural.

    Science.gov (United States)

    Xu, Zhanwei; Yan, Peifang; Liu, Kairui; Wan, Lu; Xu, Wenjuan; Li, Huixiang; Liu, Xiumei; Zhang, Z Conrad

    2016-06-01

    We report the synthesis of bis(hydroxylmethylfurfuryl)amine (BHMFA) from 5-hydroxymethylfurfural (5-HMF) by reacting 5-HMF with primary amines in the presence of homogeneous Ru(II) catalysts having sterically strained ligands. BHMFA is a group of furan-based monomers that offer great potential to form functional biopolymers with tunable properties. A range of primary amines, such as aliphatic and benzyl amines, are readily converted with 5-HMF to form the corresponding BHMFA in good yields. The reaction proceeds through reductive amination of 5-HMF with primary amine to form secondary amine, followed by reductive amination of 5-HMF with in situ generated secondary amine to produce BHMFA. PMID:27151257

  14. CCN activity of aliphatic amine secondary aerosol

    Directory of Open Access Journals (Sweden)

    X. Tang

    2014-01-01

    Full Text Available Aliphatic amines can form secondary aerosol via oxidation with atmospheric radicals (e.g. hydroxyl radical and nitrate radical. The particle composition can contain both secondary organic aerosol (SOA and inorganic salts. The fraction of organic to inorganic materials in the particulate phase influences aerosol hygroscopicity and cloud condensation nuclei (CCN activity. SOA formed from trimethylamine (TMA and butylamine (BA reactions with hydroxyl radical (OH is composed of organic material of low hygroscopicity (single hygroscopicity parameter, κ ≤ 0.25. Secondary aerosol formed from the tertiary aliphatic amine (TMA with N2O5 (source of nitrate radical, NO3, contains less volatile compounds than the primary aliphatic amine (BA aerosol. TMA + N2O5 form semi-volatile organics in low RH conditions that have κ ~ 0.20, indicative of slightly soluble organic material. As RH increases, several inorganic amine salts are formed as a result of acid-base reactions. The CCN activity of the humid TMA-N2O5 aerosol obeys Zdanovskii, Stokes, and Robinson (ZSR ideal mixing rules. Higher CCN activity (κ > 0.3 was also observed for humid BA+N2O5 aerosols compared with dry aerosol (κ ~ 0.2, as a result of the formation of inorganic salts such as NH4NO3 and butylamine nitrate (C4H11N · HNO3. Compared with TMA, BA+N2O5 reactions produce more volatile aerosols. The BA+N2O5 aerosol products under humid experiments were found to be very sensitive to the temperature within the stream-wise continuous flow thermal gradient CCN counter. The CCN counter, when set above a 21 °C temperature difference, evaporates BA+N2O5 aerosol formed at RH ≥ 30%; κ ranges from 0.4 to 0.7 and is dependent on the instrument supersaturation (ss settings. The aerosol behaves non-ideally, hence simple ZSR rules cannot be applied to the CCN results from the primary aliphatic amine system. Overall, aliphatic amine aerosol systems κ ranges from 0.2 < κ < 0.7. This work indicates that

  15. Les risques du travail

    CERN Document Server

    Thébaud-Mony, Annie

    2015-01-01

    Depuis les années 1990, les conditions de travail se sont peu à peu imposées dans le débat social. Néanmoins, la situation reste critique. Les risques traditionnels n'ont pas disparu : les manutentions lourdes, l'exposition professionnelle aux cancérogènes, au bruit ou aux vibrations demeurent répandues... De plus, certaines " améliorations " n'ont fait que déplacer et dissimuler les problèmes, telle l'externalisation des risques grâce à la sous-traitance. Dans le même temps, les transformations du travail et des modalités de gestion de la main-d'œuvre ont fragilisé les collectifs et accru l'isolement des salariés, conduisant à une montée visible de la souffrance psychique. Face à ces évolutions, il est plus que jamais nécessaire que tous les acteurs concernés, en particulier les salariés eux-mêmes et leurs représentants, s'approprient les connaissances indispensables pour améliorer la protection de la santé sur les lieux du travail. Tel est le but de ce livre, qui renouvelle int�...

  16. BIOGENIC AMINES CONTENT IN DIFFERENT WINE SAMPLES

    Directory of Open Access Journals (Sweden)

    Attila Kántor

    2015-02-01

    Full Text Available Twenty-five samples of different Slovak wines before and after filtration were analysed in order to determine the content of eight biogenic amines (tryptamine, phenylalanine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine. The method involves extraction of biogenic amines from wine samples with used dansyl chloride. Ultra-high performance liquid chromatography (UHPLC was used for determination of biogenic amines equipped with a Rapid Resolution High Definition (RRHD, DAD detectors and Extend-C18 LC column (50 mm x 3.0 mm ID, 1.8 μm particle size. In this study the highest level of biogenic amine in all wine samples represent tryptamine (TRM with the highest content 170.9±5.3 mg/L in Pinot Blanc wine. Phenylalanine (PHE cadaverine (CAD, histamine (HIS and spermidine (SPD were not detected in all wines; mainly SPD was not detected in 16 wines, HIS not detected in 14 wines, PHE and CAD not detected in 2 wines. Tyramine (TYR, spermine (SPN and putrescine (PUT were detected in all wines, but PUT and SPN in very low concentration. The worst wine samples with high biogenic amine content were Saint Laurent (BF, Pinot Blanc (S and Pinot Noir (AF.

  17. A survey of biogenic amines in vinegars.

    Science.gov (United States)

    Ordóñez, J L; Callejón, R M; Morales, M L; García-Parrilla, M C

    2013-12-01

    This paper reports the determination of biogenic amines by high-performance liquid chromatography (HPLC) and fluorescence detection after derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) in balsamic, apple, and red, white, and Sherry wine vinegars. A solid-phase extraction (SPE) with mixed-mode resins method was used before analysis. The method was successfully validated obtaining adequate values of selectivity, response linearity, precision, accuracy, and low detection and quantification limits. The total content of biogenic amines in vinegars ranged from 23.35 to 1445.2 μg/L, being lower than those reported in wines. Putrescine was the amine that showed the highest concentrations in most samples. Methylamine and phenylethylamine were not determined in any vinegar. Balsamic and "Pedro Ximénez" Sherry vinegars reached the highest amounts of biogenic amines, while apple, white and Sherry wine vinegars had the lowest concentrations. Principal component analysis using the biogenic amines as variables, allowed to separate the different kind of vinegars, excepting red vinegars. PMID:23871015

  18. Biogenic amines in raw and processed seafood

    Directory of Open Access Journals (Sweden)

    Pierina eVisciano

    2012-06-01

    Full Text Available The presence of biogenic amines in raw and processed seafood, associated with either time/temperature conditions or food technologies is discussed in the present paper from a safety and prevention point of view. In particular, storage temperature, handling practices, presence of microbial populations with decarboxylase activity and availability of free amino acids are considered the most important factors affecting the production of biogenic amines in raw seafood. On the other hand, some food technological treatments such as salting, ripening, fermentation or marination can increase the levels of biogenic amines in processed seafood. The consumption of high amount of biogenic amines, above all histamine, can result in food borne poisoning which is a worldwide problem. The European Regulation established as maximum limits for histamine, in fishery products from fish species associated with high histidine amounts, values ranging from 100 to 200 mg/kg, while for products which have undergone enzyme maturation treatment in brine, the aforementioned limits rise to 200 and 400 mg/kg. Preventive measures and emerging methods aiming at controlling the production of biogenic amines are also reported for potential application in seafood industries.

  19. Antimicrobial peptides.

    Science.gov (United States)

    Zhang, Ling-Juan; Gallo, Richard L

    2016-01-11

    Antimicrobial peptides and proteins (AMPs) are a diverse class of naturally occurring molecules that are produced as a first line of defense by all multicellular organisms. These proteins can have broad activity to directly kill bacteria, yeasts, fungi, viruses and even cancer cells. Insects and plants primarily deploy AMPs as an antibiotic to protect against potential pathogenic microbes, but microbes also produce AMPs to defend their environmental niche. In higher eukaryotic organisms, AMPs can also be referred to as 'host defense peptides', emphasizing their additional immunomodulatory activities. These activities are diverse, specific to the type of AMP, and include a variety of cytokine and growth factor-like effects that are relevant to normal immune homeostasis. In some instances, the inappropriate expression of AMPs can also induce autoimmune diseases, thus further highlighting the importance of understanding these molecules and their complex activities. This Primer will provide an update of our current understanding of AMPs. PMID:26766224

  20. Antimicrobial Peptides

    Directory of Open Access Journals (Sweden)

    Ali Adem Bahar

    2013-11-01

    Full Text Available The rapid increase in drug-resistant infections has presented a serious challenge to antimicrobial therapies. The failure of the most potent antibiotics to kill “superbugs” emphasizes the urgent need to develop other control agents. Here we review the history and new development of antimicrobial peptides (AMPs, a growing class of natural and synthetic peptides with a wide spectrum of targets including viruses, bacteria, fungi, and parasites. We summarize the major types of AMPs, their modes of action, and the common mechanisms of AMP resistance. In addition, we discuss the principles for designing effective AMPs and the potential of using AMPs to control biofilms (multicellular structures of bacteria embedded in extracellular matrixes and persister cells (dormant phenotypic variants of bacterial cells that are highly tolerant to antibiotics.

  1. Carboxylate-Assisted Iridium-Catalyzed C-H Amination of Arenes with Biologically Relevant Alkyl Azides.

    Science.gov (United States)

    Zhang, Tao; Hu, Xuejiao; Wang, Zhen; Yang, Tiantian; Sun, Hao; Li, Guigen; Lu, Hongjian

    2016-02-24

    An iridium-catalyzed C-H amination of arenes with a wide substrate scope is reported. Benzamides with electron-donating and -withdrawing groups and linear, branched, and cyclic alkyl azides are all applicable. Cesium carboxylate is crucial for both reactivity and regioselectivity of the reactions. Many biologically relevant molecules, such as amino acid, peptide, steroid, sugar, and thymidine derivatives can be introduced to arenes with high yields and 100 % chiral retention. PMID:26712274

  2. Jouer du piano

    Directory of Open Access Journals (Sweden)

    Fériel Kaddour

    2011-04-01

    Full Text Available La réflexion s’appuie dans un premier temps sur une opposition entre deux attitudes de pianistes  à l’égard du travail à l’instrument : Gould, qui revendique une séparation d’avec le clavier pour ne privilégier que la lecture; Arrau, dont la technique au contraire vise à « faire corps » avec son piano. L’étude de ces deux démarches d’interprètes conduit à une conclusion croisée : l’abstraction gouldienne n’est rien d’autre qu’un déplacement du jeu vers d’autres instruments (ceux qui servent à la prise de son et au montage de ses enregistrements ; le « faire-corps » hérité de la culture pianistique romantique est plus dialectique que fusionnel, et en cela implique une capacité de mise à distance. A partir de cette double conclusion, on tâche enfin de repenser la place du jeu à l’instrument dans la mise en œuvre d’une interprétation, en interrogeant le dialogue qui s’instaure entre la partition telle qu’elle s’écrit et le geste tel qu’il se joue.Our study leans on an opposition between two pianists' attitudes about their work with the instrument. Gould claims a necessary separation from the keyboard in order to prioritize reading. Arrau, on the contrary, relies on a technique which consists in “being one” with his piano. The analysis of these two interprets’ behaviours leads to a crossed conclusion: the gouldian abstraction is nothing else than a displacement of the playing towards another kind of instruments, the ones he uses in sound recording and cut up; Arrau’s “being one” is more dialectic than at first sight, and it therefore implies a real distancing from the piano. This constatation leads to rethink the place of the piano playing in the setting of an interpretation, and to highlight the real dialogue which develops itself between the score as it has been written and the gesture as it is played.

  3. The Role of Amines, Hydrogen Sulfide and Carbon Dioxide in the Formation of Prebiotic Macromolecules Surrounded by Membranes

    Science.gov (United States)

    Rajen, Gaurav

    2016-04-01

    in industrial amine acid gas treatment systems)- this time within a protected structure. Having reformed within a protected structure an amino acid would survive and combine into peptides as opposed to being formed in free ocean water and being degraded from exposure to direct thermal energy from the hydrothermal vents. This model predicts the size of vesicles that could move about in a likely convection cell formed within typical porous solid structures found near hydrothermal vents, as well as the wall thickness of such a vesicle from heat transfer considerations - both predictions correspond extremely well with the sizes and cell wall thicknesses of the very early forms of living cells.

  4. Exploitation of the Ornithine Effect Enhances Characterization of Stapled and Cyclic Peptides

    Science.gov (United States)

    Crittenden, Christopher M.; Parker, W. Ryan; Jenner, Zachary B.; Bruns, Kerry A.; Akin, Lucas D.; McGee, William M.; Ciccimaro, Eugene; Brodbelt, Jennifer S.

    2016-05-01

    A method to facilitate the characterization of stapled or cyclic peptides is reported via an arginine-selective derivatization strategy coupled with MS/MS analysis. Arginine residues are converted to ornithine residues through a deguanidination reaction that installs a highly selectively cleavable site in peptides. Upon activation by CID or UVPD, the ornithine residue cyclizes to promote cleavage of the adjacent amide bond. This Arg-specific process offers a unique strategy for site-selective ring opening of stapled and cyclic peptides. Upon activation of each derivatized peptide, site-specific backbone cleavage at the ornithine residue results in two complementary products: the lactam ring-containing portion of the peptide and the amine-containing portion. The deguanidination process not only provides a specific marker site that initiates fragmentation of the peptide but also offers a means to unlock the staple and differentiate isobaric stapled peptides.

  5. Exploitation of the Ornithine Effect Enhances Characterization of Stapled and Cyclic Peptides.

    Science.gov (United States)

    Crittenden, Christopher M; Parker, W Ryan; Jenner, Zachary B; Bruns, Kerry A; Akin, Lucas D; McGee, William M; Ciccimaro, Eugene; Brodbelt, Jennifer S

    2016-05-01

    A method to facilitate the characterization of stapled or cyclic peptides is reported via an arginine-selective derivatization strategy coupled with MS/MS analysis. Arginine residues are converted to ornithine residues through a deguanidination reaction that installs a highly selectively cleavable site in peptides. Upon activation by CID or UVPD, the ornithine residue cyclizes to promote cleavage of the adjacent amide bond. This Arg-specific process offers a unique strategy for site-selective ring opening of stapled and cyclic peptides. Upon activation of each derivatized peptide, site-specific backbone cleavage at the ornithine residue results in two complementary products: the lactam ring-containing portion of the peptide and the amine-containing portion. The deguanidination process not only provides a specific marker site that initiates fragmentation of the peptide but also offers a means to unlock the staple and differentiate isobaric stapled peptides. PMID:26864791

  6. Role of acetylation and charge in antimicrobial peptides based on human beta-defensin-3.

    Science.gov (United States)

    Papanastasiou, Emilios Andrew; Hua, Quyen; Sandouk, Aline; Son, U Hyon; Christenson, Andrew James; Van Hoek, Monique Louise; Bishop, Barney Michael

    2009-07-01

    Cationic antimicrobial peptides are an evolutionarily ancient and essential element of innate immunity in higher organisms. The precise mechanism by which these peptides exert their antimicrobial activity on bacteria is not well understood. Decapeptides based on the C-terminus of human beta-defensin-3 were designed and evaluated to study the role of charge in defining the antimicrobial activity and selectivity of these peptides against Escherichia coli. Acetylated derivatives of these peptides were prepared in order to further evaluate how positively charged primary amines contribute to potency in these small antimicrobial peptides. These peptides enabled us to explore the relationship between net charge, charge distribution and antimicrobial activity. While the results indicate that net charge is a major factor in antimicrobial activity in these peptides, the actual relationship between charge and potency appears to be more complex.

  7. SUICIDAL IDEATION AND BIOGENIC AMINES IN DEPRESSION*

    OpenAIRE

    Palaniappan, V.; V. Ramachandran; Somasundaram, O.

    1983-01-01

    SUMMARY This report is based on the study of 40 depressives in an attempt to explore the possible association between the suicidal ideas and the biogenic amines. The severity of suicidal ideas was measured on Hamilton Depressive Rating Scale and their amine metabolites were measured (MHFG, HVA and 5 HIAA) in urine and C.S.F. It was observed that the level of 5 HIAA, and Serotonin (5 HT) was more related to suicidal ideas and was inversely related. The probable associations between these are d...

  8. CCN activity of aliphatic amine secondary aerosol

    Science.gov (United States)

    Tang, X.; Price, D.; Praske, E.; Vu, D.; Purvis-Roberts, K.; Silva, P. J.; Cocker, D. R., III; Asa-Awuku, A.

    2014-01-01

    Aliphatic amines can form secondary aerosol via oxidation with atmospheric radicals (e.g. hydroxyl radical and nitrate radical). The particle composition can contain both secondary organic aerosol (SOA) and inorganic salts. The fraction of organic to inorganic materials in the particulate phase influences aerosol hygroscopicity and cloud condensation nuclei (CCN) activity. SOA formed from trimethylamine (TMA) and butylamine (BA) reactions with hydroxyl radical (OH) is composed of organic material of low hygroscopicity (single hygroscopicity parameter, κ ≤ 0.25). Secondary aerosol formed from the tertiary aliphatic amine (TMA) with N2O5 (source of nitrate radical, NO3), contains less volatile compounds than the primary aliphatic amine (BA) aerosol. TMA + N2O5 form semi-volatile organics in low RH conditions that have κ ~ 0.20, indicative of slightly soluble organic material. As RH increases, several inorganic amine salts are formed as a result of acid-base reactions. The CCN activity of the humid TMA-N2O5 aerosol obeys Zdanovskii, Stokes, and Robinson (ZSR) ideal mixing rules. Higher CCN activity (κ > 0.3) was also observed for humid BA+N2O5 aerosols compared with dry aerosol (κ ~ 0.2), as a result of the formation of inorganic salts such as NH4NO3 and butylamine nitrate (C4H11N · HNO3). Compared with TMA, BA+N2O5 reactions produce more volatile aerosols. The BA+N2O5 aerosol products under humid experiments were found to be very sensitive to the temperature within the stream-wise continuous flow thermal gradient CCN counter. The CCN counter, when set above a 21 °C temperature difference, evaporates BA+N2O5 aerosol formed at RH ≥ 30%; κ ranges from 0.4 to 0.7 and is dependent on the instrument supersaturation (ss) settings. The aerosol behaves non-ideally, hence simple ZSR rules cannot be applied to the CCN results from the primary aliphatic amine system. Overall, aliphatic amine aerosol systems κ ranges from 0.2 CCN activity. Thermal gradient CCN

  9. Corrosion inhibition of brass by aliphatic amines

    International Nuclear Information System (INIS)

    Aliphatic amines hexylamine (HCA), octylamine (OCA) and decylamine (DCA) have been used as corrosion inhibitors for (70/30) brass in 0.I M HCIO4. The inhibitor efficiency (%P) calculated using weight loss, Tafel extrapolation, linear polarization and impedance methods was found to be in the order DCA> OCA> HCA. These adsorb on brass surface following bockris-swinkels' isotherm. DCA, OCA and HCA displaced 4, 3 and 2 molecules of water from interface respectively. Displacement of water molecules brought a great reorganization of double layer at the interface. These amines during corrosion form complexes with dissolved zinc and copper ions.(Author)

  10. Methods for attaching polymerizable ceragenins to water treatment membranes using amine and amide linkages

    Energy Technology Data Exchange (ETDEWEB)

    Hibbs, Michael; Altman, Susan J.; Jones, Howland D.T.; Savage, Paul B.

    2013-10-15

    This invention relates to methods for chemically grafting and attaching ceragenin molecules to polymer substrates; methods for synthesizing ceragenin-containing copolymers; methods for making ceragenin-modified water treatment membranes and spacers; and methods of treating contaminated water using ceragenin-modified treatment membranes and spacers. Ceragenins are synthetically produced antimicrobial peptide mimics that display broad-spectrum bactericidal activity. Alkene-functionalized ceragenins (e.g., acrylamide-functionalized ceragenins) can be attached to polyamide reverse osmosis membranes using amine-linking, amide-linking, UV-grafting, or silane-coating methods. In addition, silane-functionalized ceragenins can be directly attached to polymer surfaces that have free hydroxyls.

  11. Le sacre du printemps

    Directory of Open Access Journals (Sweden)

    Denise Pumain

    2002-03-01

    Full Text Available Cybergeo aura six ans en avril : dans la réalité du virtuel, dans l'univers récent et fluctuant de la publication en ligne, cela fait de nous, tout à la fois, des pionniers et des vétérans. De façon plus surprenante, il se trouve que nous sommes aussi uniques : parmi toutes les revues électroniques de sciences sociales, aucune ne combine comme Cybergeo ancienneté, publication exclusivement électronique, liberté d'accès au texte intégral, édition et gestion par des chercheurs, et comité de lec...

  12. Femmes du Maghreb

    OpenAIRE

    Leduc, Claudine; Fine, Agnès

    2003-01-01

    L'actualité, comme toujours, suscite la réflexion historique. Devant le déchaînement de la violence en Algérie, CLIO avait programmé, dès sa fondation en 1995, un numéro consacré aux Femmes d'Algérie où se seraient exprimés des chercheurs (euses) d'Algérie. C'était pour son comité de rédaction une façon de dire, comme il le pouvait, sa solidarité à ceux qui vivaient dans la quotidienneté de la terreur. Il a demandé à Djamila Amrane, titulaire depuis 1994 du poste d'« Histoire des Femme...

  13. La musique du malentendu

    OpenAIRE

    Abbrugiati, Raymond

    2011-01-01

    La « petite musique » du style tabucchien rencontre souvent la musique tout court : personnages musiciens, musique en arrière-plan des récits, ou au centre de la trame narrative, donnent une projection artistique au problème d’une harmonie impossible entre des êtres qui “n’entendent” pas les événements de la même manière. Il s’agira d’examiner les récits de Tabucchi où la musique joue un rôle flagrant, pour définir ce rôle et, au-delà de leur diégèse propre, de comprendre de quelle métaphore ...

  14. Enantioselective catalytic syntheses of alpha-branched chiral amines

    DEFF Research Database (Denmark)

    Brase, S.; Baumann, T.; Dahmen, S.;

    2007-01-01

    Chiral amines play a pivotal role in fine chemical and natural product syntheses and the design of novel materials.......Chiral amines play a pivotal role in fine chemical and natural product syntheses and the design of novel materials....

  15. A molecular probe for the optical detection of biogenic amines

    OpenAIRE

    Lee, Boram; Scopelliti, Rosario; Severin, Kay

    2011-01-01

    A coumarin derivative was employed for the detection of biogenic amines in buffered aqueous solution by UV-Vis or fluorescence spectroscopy. Incorporated in a polymeric matrix, the dye can also be used for the optical detection of gaseous amines.

  16. OPERATION DU FOISONNEMENT

    Directory of Open Access Journals (Sweden)

    Gholamreza Djelveh

    2010-04-01

    Full Text Available Mousses alimentaires sont un sous-ensemble des aliments connus sous le nom de produits fouettés ou des produits aérés. Ils sont des produits formulés avec des qualités telles que la légèreté et la souplesse et sont principalement consommés à l'apéritif ou au dessert. Les produits en mousse obtenue par dispersion d'un gaz dans une matrice alimentaire (la phase continue ont connu un développement croissant au cours des années 80 et 90. Le processus d'aération liés à leurs activités de production est appelée l'expansion ou à fouetter. Le document présente les principaux-paramètres du procédé du point permanent de la formulation, la mise en œuvre processus dans les installations pilotes et à l'échelle industrielle, la caractérisation des produits finis, la base énergétique de l'échelle de processus en place, et le lien entre la formulation, émulsion préparation de l'expansion. Cette vue d'ensemble de l'opération d'expansion continue, nous a permis de mettre en évidence le fait qu'il ya des opérations de l'unité encore mal décrite par le génie des procédés et pour lesquels les méthodes et outils pour l'extrapolation et la prédiction sont encore à leurs balbutiements.

  17. Factors influencing biogenic amines accumulation in dairy products

    OpenAIRE

    Daniel M. eLinares; Beatriz edel Río; Victor eLadero; Noelia eMartínez; María eFernández; María Cruz eMartín; Miguel A. eAlvarez

    2012-01-01

    Fermented foods are within the food products more often complained of having caused biogenic amines poisoning. Concerning milk-based fermented foods, cheese is the main product likely to contain significant levels of biogenic amines, specially tyramine, histamine and putrescine. Prompted by the increasing awareness of the risks related to dietary uptake of high biogenic amine loads, in this review we report about cheese elaboration and processing technological aspects affecting biogenic amine...

  18. 40 CFR 721.640 - Amine substituted metal salts.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Amine substituted metal salts. 721.640... Substances § 721.640 Amine substituted metal salts. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substances identified generically as amine substituted metal...

  19. 40 CFR 721.750 - Aromatic amine compound.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Aromatic amine compound. 721.750... Substances § 721.750 Aromatic amine compound. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance aromatic amine compound (PMN P-86-334) is subject to reporting...

  20. 40 CFR 721.9490 - Coco alklydimethyl amine salts (generic).

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Coco alklydimethyl amine salts... Specific Chemical Substances § 721.9490 Coco alklydimethyl amine salts (generic). (a) Chemical substances... coco alkyldimethyl amine salts (PMNs P-98-412/414/415/416/417) are subject to reporting under...

  1. A Novel Lactic Acid Bacteria Growth-stimulating Peptide from Broad Bean (Vicia faba .) Protein Hydrolysates

    OpenAIRE

    Ping Xiao; Yuan Liu; Rizwan-ur-Rehman; Ran Kang; Yanping Wang

    2015-01-01

    In this study, broad bean protein hydrolysates (BPH) produced by alcalase with strong-stimulating activity for lactic acid bacteria (LAB) was first time reported. In order to obtain the key peptide that have growth-stimulating activity for lactic acid bacteria (LAB), gel filtration chromatography and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) were applied to isolate and purify the peptides from BPH. Finally, F4-2 elicited the highest activity for LAB, corresponding to amin...

  2. Facile synthesis of aliphatic isothiocyanates and thioureas on solid phase using peptide coupling reagents

    DEFF Research Database (Denmark)

    Boas, Ulrik; Andersen, Heidi Gertz; Christensen, Jørn B.;

    2004-01-01

    Peptide coupling reagents can be used as versatile reagents for the formation of aliphatic isothiocyanates and thioureas on solid phase from the corresponding solid-phase anchored aliphatic primary amines. The formation of the thioureas is fast and highly chemoselective, and proceeds via formation...... of the intermediate isothiocyanate. The isothiocyanate and subsequent thiourea formation take place under standard peptide coupling conditions using carbon disulfide as the 'amino acid'. The thioureas are released from the resin and isolated in moderate to high yields....

  3. Fate and biodegradability of sulfonated aromatic amines

    NARCIS (Netherlands)

    Tan, N.C.G.; Leeuwen, van A.; Voorthuizen, van E.M.; Slenders, P.; Prenafeta, F.X.; Temmink, H.; Lettinga, G.; Field, J.A.

    2005-01-01

    Ten sulfonated aromatic amines were tested for their aerobic and anaerobic biodegradability and toxicity potential in a variety of environmental inocula. Of all the compounds tested, only two aminobenzenesulfonic acid (ABS) isomers, 2- and 4-ABS, were degraded. The observed degradation occurred only

  4. New adducts of Lapachol with primary amines

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Mirelly D.F.; Litivack-Junior, Jose T.; Antunes, Roberto V.; Silva, Tania M.S.; Camara, Celso A., E-mail: ccelso@dq.ufrpe.b [Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE (Brazil). Dept. de Quimica

    2011-07-01

    New adducts of lapachol with neat primary aliphatic amines were obtained in a solvent-free reaction in good to reasonable yields (52 to 88%), at room temperature. The new compounds containing a phenazine moiety were obtained from suitable functionalized aminoalkyl compounds, including ethanolamine, 3-propanolamine, 2-methoxy-ethylamine, 3-methoxy-propylamine, n-butylamine and 2-phenetylamine. (author)

  5. Simultaneous structure-activity studies and arming of natural products by C-H amination reveal cellular targets of eupalmerin acetate

    Science.gov (United States)

    Li, Jing; Cisar, Justin S.; Zhou, Cong-Ying; Vera, Brunilda; Williams, Howard; Rodríguez, Abimael D.; Cravatt, Benjamin F.; Romo, Daniel

    2013-06-01

    Natural products have a venerable history of, and enduring potential for the discovery of useful biological activity. To fully exploit this, the development of chemical methodology that can functionalize unique sites within these complex structures is highly desirable. Here, we describe the use of rhodium(II)-catalysed C-H amination reactions developed by Du Bois to carry out simultaneous structure-activity relationship studies and arming (alkynylation) of natural products at ‘unfunctionalized’ positions. Allylic and benzylic C-H bonds in the natural products undergo amination while olefins undergo aziridination, and tertiary amine-containing natural products are converted to amidines by a C-H amination-oxidation sequence or to hydrazine sulfamate zwitterions by an unusual N-amination. The alkynylated derivatives are ready for conversion into cellular probes that can be used for mechanism-of-action studies. Chemo- and site-selectivity was studied with a diverse library of natural products. For one of these—the marine-derived anticancer diterpene, eupalmerin acetate—quantitative proteome profiling led to the identification of several protein targets in HL-60 cells, suggesting a polypharmacological mode of action.

  6. Role of Amine Functionality for CO2 Chemisorption on Silica.

    Science.gov (United States)

    Hahn, Maximilian W; Jelic, Jelena; Berger, Edith; Reuter, Karsten; Jentys, Andreas; Lercher, Johannes A

    2016-03-01

    The mechanism of CO2 adsorption on primary, secondary, and bibasic aminosilanes synthetically functionalized in porous SiO2 was qualitatively and quantitatively investigated by a combination of IR spectroscopy, thermogravimetry, and quantum mechanical modeling. The mode of CO2 adsorption depends particularly on the nature of the amine group and the spacing between the aminosilanes. Primary amines bonded CO2 preferentially through the formation of intermolecular ammonium carbamates, whereas CO2 was predominantly stabilized as carbamic acid, when interacting with secondary amines. Ammonium carbamate formation requires the transfer of the carbamic acid proton to a second primary amine group to form the ammonium ion and hence two (primary) amine groups are required to bind one CO2 molecule. The higher base strength of secondary amines enables the stabilization of carbamic acid, which is thereby hindered to interact further with nearby amine functions, because their association with Si-OH groups (either protonation or hydrogen bonding) does not allow further stabilization of carbamic acid as carbamate. Steric hindrance of the formation of intermolecular ammonium carbamates leads to higher uptake capacities for secondary amines functionalized in porous SiO2 at higher amine densities. In aminosilanes possessing a primary and a secondary amine group, the secondary amine group tends to be protonated by Si-OH groups and therefore does not substantially interact with CO2. PMID:26700549

  7. Le collisionneur du futur?

    CERN Multimedia

    CERN Audiovisual Service

    2009-01-01

    Pourquoi deux études parallèles pour un même collisionneur linéaire ? Loin d’être un double effort et un gaspillage de ressources, ces deux études s’inscrivent dans une stratégie de complémentarité afin de fournir la meilleure technologie requise par la physique du futur. Vendredi 12 juin a eu lieu au CERN la première réunion conjointe CLIC et ILC. Elle n’a pas été avare de bons résultats et d’importantes décisions. Le Collisionneur Linéaire International (ILC) et le Collisionneur Linéaire Compact (CLIC) sont deux études qui font, tous deux, appel à des technologies de pointe. A première vue en compétition, les deux études sont en réalité complémentaires et elles ont un objectif commun : proposer dans les plus brefs délais et au moindre coût, l‘accélérateur linéaire le mieux adapté pour prendre le relais de la physique des très hautes énergies après le LHC.

  8. Le bal du loup

    CERN Multimedia

    Happy Children's Home

    2013-01-01

    The Bord'eau amateur theatre group will graciously perform a play of their creation Le bal du loup Saturday 19 October 2013 at 20:00 Sunday 20 October at 17:00 in the Théâtre des Grottes Rue Louis Favre 43, 1201 Genève Children from age 12 upwards. Summary: The new-elected mayoress of a small village would like to clean up the town by prohibiting alcohol and getting rid of its prostitutes. Then along comes « Massimo Lupo » the pimp... The performances will be given to support the Happy Children's Home charity, which runs a foster-home in Pokhara for Nepali children:  http://www.happychildrenshome.org/ Admission : minimum charge of 10 CHF per person requested, to cover the cost of technical assistance and theatre rental. Any profit will be used solely for the foster-home. At the end of each performance members of the HCH charity will be happy to answer any questions you may have. The theatre has 86 seats, thank you for reserv...

  9. A caged substrate peptide for matrix metalloproteinases.

    Science.gov (United States)

    Decaneto, Elena; Abbruzzetti, Stefania; Heise, Inge; Lubitz, Wolfgang; Viappiani, Cristiano; Knipp, Markus

    2015-02-01

    Based on the widely applied fluorogenic peptide FS-6 (Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2; Mca = methoxycoumarin-4-acetyl; Dpa = N-3-(2,4-dinitrophenyl)l-α,β-diaminopropionyl) a caged substrate peptide Ac-Lys-Pro-Leu-Gly-Lys*-Lys-Ala-Arg-NH2 (*, position of the cage group) for matrix metalloproteinases was synthesized and characterized. The synthesis implies the modification of a carbamidated lysine side-chain amine with a photocleavable 2-nitrobenzyl group. Mass spectrometry upon UV irradiation demonstrated the complete photolytic cleavage of the protecting group. Time-resolved laser-flash photolysis at 355 nm in combination with transient absorption spectroscopy determined the biphasic decomposition with τa = 171 ± 3 ms (79%) and τb = 2.9 ± 0.2 ms (21%) at pH 6.0 of the photo induced release of the 2-nitrobenzyl group. The recombinantly expressed catalytic domain of human membrane type I matrix metalloproteinase (MT1-MMP or MMP-14) was used to determine the hydrolysis efficiency of the caged peptide before and after photolysis. It turned out that the cage group sufficiently shields the peptide from peptidase activity, which can be thus controlled by UV light.

  10. Effets du fluor et du phosphogypse chez les organismes marins

    OpenAIRE

    Martin, J. L.; Le Guellec, Anne-marie; Cosson, R

    1982-01-01

    Dans le cadre général des recherches visant à déterminer l'action des effluents industriels et plus précisément du fluor dans le cas de cette étude, le programme dont les résultats sont présentés avait pour but de déterminer les effets de cet élément sur les organismes marins. Trois aspects essentiels ont été envisagés : - Effets létaux du fluor ; - effets du fluor sur certaines fonctions éthologiques ; - bio-accumulation de l'élément par les organismes marins.

  11. Effect of aminated gelatin on the nasal absorption of insulin in rats.

    Science.gov (United States)

    Seki, Toshinobu; Kanbayashi, Hiroshi; Nagao, Tomonobu; Chono, Sumio; Tomita, Mikio; Hayashi, Masahiro; Tabata, Yasuhiko; Morimoto, Kazuhiro

    2005-03-01

    Absorption enhancers, which increase the permeability of drugs through epithelial membranes without damaging them, are especially useful for intranasal administration of peptide drugs. In this study, aminated gelatins, candidate enhancers, having different numbers of amino groups were prepared from gelatin (H-gelatin, isoelectric point = 9.0, MW 100 kDa) and a partial gelatin hydrolysate (L-gelatin, isoelectric point = 8.0, MW 5 kDa), and the enhancing effects on the nasal absorption of insulin, used as a model peptide drug, and 5(6)-carboxyfluorescein (CF), a paracellular marker, were examined in rats. The enhancing effect on insulin and CF depends on the MW and number of amino groups. A high correlation between the enhancing effects on insulin and CF was observed and this suggests that an increase in the paracellular permeability is the mechanism governing the nasal absorption-enhancement of aminated gelatins, at least as far as insulin and CF are concerned. The enhancing mechanism might be shared with other cationic polymers having absorption-enhancing effects.

  12. Biomimetic peptide-based models of [FeFe]-hydrogenases: utilization of phosphine-containing peptides.

    Science.gov (United States)

    Roy, Souvik; Nguyen, Thuy-Ai D; Gan, Lu; Jones, Anne K

    2015-09-01

    Two synthetic strategies for incorporating diiron analogues of [FeFe]-hydrogenases into short peptides via phosphine functional groups are described. First, utilizing the amine side chain of lysine as an anchor, phosphine carboxylic acids can be coupled via amide formation to resin-bound peptides. Second, artificial, phosphine-containing amino acids can be directly incorporated into peptides via solution phase peptide synthesis. The second approach is demonstrated using three amino acids each with a different phosphine substituent (diphenyl, diisopropyl, and diethyl phosphine). In total, five distinct monophosphine-substituted, diiron model complexes were prepared by reaction of the phosphine-peptides with diiron hexacarbonyl precursors, either (μ-pdt)Fe2(CO)6 or (μ-bdt)Fe2(CO)6 (pdt = propane-1,3-dithiolate, bdt = benzene-1,2-dithiolate). Formation of the complexes was confirmed by UV/Vis, FTIR and (31)P NMR spectroscopy. Electrocatalysis by these complexes is reported in the presence of acetic acid in mixed aqueous-organic solutions. Addition of water results in enhancement of the catalytic rates.

  13. Human peptide transporters

    DEFF Research Database (Denmark)

    Nielsen, Carsten Uhd; Brodin, Birger; Jørgensen, Flemming Steen;

    2002-01-01

    Peptide transporters are epithelial solute carriers. Their functional role has been characterised in the small intestine and proximal tubules, where they are involved in absorption of dietary peptides and peptide reabsorption, respectively. Currently, two peptide transporters, PepT1 and PepT2...

  14. Amine chemistry. Update on impact on resin

    Energy Technology Data Exchange (ETDEWEB)

    Bachman, Gregory; Kellogg, Douglas [Siemens Industry, Inc., Rockford, IL (United States). Technology and Lab Services; Wilkes, Marty [Siemens Industry, Inc., Rockford, IL (United States). Water Technologies Div.

    2012-03-15

    Impurity removal in the steam cycle and the associated prevention of corrosion and/or fouling of system components are the goals of ion exchange resins. However, in many instances (such as a switch to amine chemistry or a change in product specifications), resins do not remove, and, in fact, contribute impurities to the steam cycle. This paper reviews recent data compiled to determine the direct and indirect effects of amines on ion exchange resins used in the power industry. Water chemistries have improved in recent years, in large part due to changes in chemistry and resins, but it is necessary to continue to develop products, processes and techniques to reduce impurities and improve overall water chemistry in power plant systems. (orig.)

  15. Thermodynamics of mixtures containing amines: XIII. Application of the ERAS model to cyclic amine + alkane mixtures

    Energy Technology Data Exchange (ETDEWEB)

    González, Juan Antonio, E-mail: jagl@termo.uva.es; Sanz, Luis Felipe; García De La Fuente, Isaías; Cobos, José Carlos

    2013-12-10

    Graphical abstract: - Highlights: • Primary or secondary cyclic amine + alkane mixtures are investigated using ERAS. • ERAS parameters are given. Relatively high X{sub AB} values remark the importance of physical interactions. • ERAS parameters are consistent with those of primary or secondary (linear or aromatic) amines. • H{sub m}{sup E}, V{sub m}{sup E}, and C{sub pm}{sup E} data reveal the existence of physical interactions and structural effects. • ERAS correctly describes H{sub m}{sup E}, V{sub m}{sup E}, C{sub pm}{sup E}, G{sub m}{sup E} and the enthalpy of vaporization of pure amines. - Abstract: Primary or secondary cyclic amine + alkane mixtures have been investigated in the framework of the ERAS (Extended Real Associated Solution) model. The corresponding ERAS parameters are reported. All the amines considered have the same equilibrium constant (K{sub A} = 0.75). Cyclopropylamine, cyclopentylamine, cyclohexylamine and pyrrolidine are characterized by the same enthalpy of self-association (Δh{sub A}{sup *}=−15 kJ mol{sup −1}). Piperidine and hexamethyleneimine show a less negative Δh{sub A}{sup *} value (−13 kJ mol{sup −1}). Experimental data on excess enthalpies, H{sub m}{sup E}, volumes, V{sub m}{sup E}, and isobaric heat capacities, C{sub pm}{sup E}, reveal the existence of physical interactions and structural effects in the studied solutions. The latter lead to values of self-association of pure amines, Δv{sub A}{sup *}, which may depend on the solvent in systems with a given amine. Although the model overestimates the Δh{sub A}{sup *} values, the relatively high values of the physical parameters X{sub AB} remark the importance of physical interactions. ERAS describes correctly the excess functions H{sub m}{sup E}, V{sub m}{sup E}, C{sub pm}{sup E} and G{sub m}{sup E} (molar Gibbs energy), and the enthalpy of vaporization of pure amines. Nevertheless, discrepancies with experimental data are found for the concentration

  16. L'administration du travail et la production du droit du travail (1906-1960). : Note de synthèse du rapport de recherche

    OpenAIRE

    Le Crom, Jean-Pierre

    2007-01-01

    Synthèse d'un rapport de recherche dont l'objectif est d'appréhender le rôle de l'administration centrale du travail dans la production du droit du travail. Deux dimensions sont explorées : le profil des rédacteurs et l'organisation des structures.

  17. O-Maltosylation of Heterocyclic Ketene Aminals

    Institute of Scientific and Technical Information of China (English)

    XU,Zhan-Hui(徐占辉); HUANG,Zhi-Tang(黄志镗)

    2002-01-01

    The stereoselective synthesis of O-maltosides by reacting benzoyl-substituted heterocyclic ketene aminals 1 or 2 with acetylated maltosyl bromide 3 was investigated. Compounds 1 or 2 reacted with 3 in the presence of mercuric cyanide to give O-maltosides 4 or 5 with E-configuration. While 1 reacted with 3 in the presence of calcium hydride to give O-maltosides 6 with Zconfiguration.

  18. Rate phenomena in uranium extraction by amines

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, C. F.; McDowell, W. J.

    1979-01-01

    Kinetics studies and other rate measurements are reviewed in the amine extraction of uranium and of some other related and associated metal ions. Equilibration is relatively fast in the uranium sulfate systems most important to uranium hydrometallurgy. Significantly slow equilibration has been encountered in some other systems. Most of the recorded rate information, both qualitative and quantitative, has come from exploratory and process-development work, while some kinetics studies have been directed specifically toward elucidation of extraction mechanisms. 71 references.

  19. Biogenic Amines in Raw and Processed Seafood

    OpenAIRE

    Visciano, Pierina; Schirone, Maria; Tofalo, Rosanna; Suzzi, Giovanna

    2012-01-01

    The presence of biogenic amines (BAs) in raw and processed seafood, associated with either time/temperature conditions or food technologies is discussed in the present paper from a safety and prevention point of view. In particular, storage temperature, handling practices, presence of microbial populations with decarboxylase activity and availability of free amino acids are considered the most important factors affecting the production of BAs in raw seafood. On the other hand, some food techn...

  20. Charles Masson, Droit du sol

    OpenAIRE

    Harzoune, Mustapha

    2013-01-01

    À Mayotte, collectivité d’outre-mer en passe de devenir le 101e département français en 2011, le droit commun français a vocation à s’appliquer. Pour autant, en matière d’entrée et de séjour des étrangers, de droit du travail et de protection sociale, tel n’est pas encore le cas. François Baroin en septembre 2005 et Christian Estrosi en février 2007 voulaient étendre cette exception au droit du sol en privant les étrangers nés à Mayotte du droit de devenir français. Il faut dire qu’il y aurai...

  1. Traitement automatique du langage naturel

    OpenAIRE

    BADREDDINE, I

    2011-01-01

    Cette étude s'intéresse particulièrement aux exigences de sécurité et à une méthode d'extraction des exigences. Pour cette extraction, nous nous sommes penchés sur le traitement automatique du langage naturel. Une discipline, à la frontière de la linguistique, de l'informatique et de l'intelligence artificielle, qui concerne l'application de programme informatique à tous les aspects du langage humain (écrit et/ou parlé). Le but de l'étude du TALN est d'explorer les méthodes et de connaître la...

  2. Rett syndrome - Stimulation of endogenous biogenic amines

    Science.gov (United States)

    Pelligra, R.; Norton, R. D.; Wilkinson, R.; Leon, H. A.; Matson, W. R.

    1992-01-01

    Transient hypercapnic hyperoxemia was induced in two Rett syndrome children by the administration of a gaseous mixture of 80 percent O2 and 20 percent CO2. Time course studies of neurotransmitters and their metabolites showed an immediate and marked increase in central biogenic amine turnover following inhalation of the gas mixture. The increased turnover of biogenic amines was associated with improved clinical changes. This suggests a coupled relationship and provides further support for an etiological role of neurotransmitter dysfunction in Rett syndrome. In a complementary study, elevation of pulmonary CO2 by application of a simple rebreathing device resulted in improvement of abnormal blood gases and elimination of the Cheyne-Stokes-like respiratory pattern of the Rett syndrome. Near normalization of the EEG occurred when a normal respiratory pattern was imposed by means of a respirator. Taken together, these results lead to the preliminary conclusion that cerebral hypoxemia secondary to abnormal respiratory function may contribute to diminished production of biogenic amines in Rett syndrome.

  3. Les paradoxes du littoral Swahili

    Directory of Open Access Journals (Sweden)

    François Bart

    2008-10-01

    Full Text Available Le contact entre l'océan Indien occidental et le continent africain a forgé l'identité et l'originalité du littoral swahili. Cette interface, longue d'environ 2 000 kilomètres, du sud de la Somalie au nord du Mozambique, constitue un monde original où, au gré de traditions anciennes de circulation maritime et continentale se sont diffusées et mêlées des influences culturelles venues du continent africain, de la péninsule arabique et de la péninsule indienne. Le développement du continent, du littoral et des archipels s'inscrit dans une double logique de fragmentation et d'ouverture au monde. Dans cette vaste aire géographique, le rôle de relais des îles et des axes de pénétration continentale à partir des ports est essentiel.The identity and the original features of the Swahili seashore mainly proceed from the contact of African mainland and western indian Ocean. This coastal interface, stretching over 2 000 kilometres from southern Somalia to northern Mozambique, is characterized by ancient traditions of trade movements through maritime areas and mainland as well, which spread and mixed different cultural influences originating from Africa and both Arabic and Indian peninsulas. The development of this area is linked to both fragmentation and opening dynamics. In such a wide area, the relaying function of islands and continental corridors from main harbours is very important.

  4. On the role of small amines in zeolite synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Rollmann, L.D.; Schlenker, J.L.; Lawton, S.L.; Kennedy, C.L.; Kennedy, G.J. [Mobil Technology Co., Paulsboro, NJ (United States). Strategic Research Center; Doren, D.J. [Univ. of Delaware, Newark, DE (United States). Dept. of Chemistry and Biochemistry

    1999-08-26

    A crystallization study was undertaken to relate the structure of small amines to the zeolite product obtained. At SiO{sub 2}/Al{sub 2}O{sub 3} = 200, certain noncyclic amines (some not previously recognized) were specific for the formation of 10-ring zeolites, e.g., ZSM-22 and ZSM-23. For example, ethylmethylamine and diethylamine yielded highly crystalline ZSM-22, while ethylamine and isopropylamine yielded ZSM-12. At lower SiO{sub 2}/Al{sub 2}O{sub 3} ratios (40), a different set of zeolites was obtained, as expected, ZSM-35 and MCM-22. Again, a specific relationship was observed between the amine employed and the zeolite obtained. In both SiO{sub 2}/Al{sub 2}O{sub 3} regimes, several amines played non-structure-specific pore- and pH-stabilizing roles and yielded ZSM-5. With relatively few exceptions, the zeolite products were fully porefilled with amine and low in Na (Na/Al < 1). Computer simulations confirmed a pore-stabilizing role for both structure-specific and non-structure-specific amines. At the same time, among structure-specific (structure-directing) amines, they showed that the interaction energy differences between alternative amine-pore combinations were often quite small (0.5--2.0 kcal/mol, for example). Nevertheless, the changes in amine-pore interaction energies for this group of amines consistently tracked the changes in zeolite products observed.

  5. La promesse du bonheur : introduction

    OpenAIRE

    Ahmed, Sara

    2016-01-01

    Figure de la phénoménologie queer, Sara Ahmed interroge au fil de son œuvre les injonctions et identifications que produisent des affects spécifiques pour mieux penser l’ampleur politique des mouvements féministes, queer et noirs. Dans le prolongement d’un travail sur les politiques culturelles des émotions (2004), elle pose ici les bases d’une critique de la pensée du bonheur. La promesse du bonheur, dont est extraite la présente introduction, interroge ce que produit le désir de bonheur. Me...

  6. Des usages du constructivisme (2)

    OpenAIRE

    Fleury-Vilatte, Béatrice; Walter, Jacques

    2013-01-01

    Dans cette deuxième série d’articles consacrés au constructivisme, Bernard Delforce, Isabelle Gavillet et Nicolas Pélissier prolongent la discussion menée par Roger Bautier, Denis Benoit et Roselyne Koren sur les usages de la notion par des chercheurs travaillant dans le domaine du journalisme, quitte à s’interroger sur les effets d’une telle centration. Si l’approche historique et le recours aux sciences de l’information et de la communication (SIC) et aux sciences du langage nourrissaient l...

  7. Dužnost umiranja

    OpenAIRE

    Czerny Urban, Milica; Baccarini, Elvio

    2010-01-01

    U suvremenim raspravama o eutanaziji i liječnički potpomognutom samoubojstvu, te odustajanju od tretmana, osim uobičajenih razloga koji se temelje na slobodi i izbjegavanju patnje osobe koja traži ubrzavanje smrti, postoji i teza o dužnosti umiranja. S obzirom na to da je pojedinac ujedno i član zajednice, odnosno da ima obitelj i voljene, nužno je raspraviti o slučajevima kada bolest izaziva značajne teškoće za život njegovih bližnjih. Smatramo kako i pacijentova pravedna prosudba o dužnosti...

  8. Total Chemical Synthesis of a Heterodimeric Interchain Bis-Lactam-Linked Peptide: Application to an Analogue of Human Insulin-Like Peptide 3

    Science.gov (United States)

    Karas, John; Shabanpoor, Fazel; Hossain, Mohammed Akhter; Wade, John D.; Scanlon, Denis B.

    2013-01-01

    Nonreducible cystine isosteres represent important peptide design elements in that they can maintain a near-native tertiary conformation of the peptide while simultaneously extending the in vitro and in vivo half-life of the biomolecule. Examples of these cystine mimics include dicarba, diselenide, thioether, triazole, and lactam bridges. Each has unique physicochemical properties that impact upon the resulting peptide conformation. Each also requires specific conditions for its formation via chemical peptide synthesis protocols. While the preparation of peptides containing two lactam bonds within a peptide is technically possible and reported by others, to date there has been no report of the chemical synthesis of a heterodimeric peptide linked by two lactam bonds. To examine the feasibility of such an assembly, judicious use of a complementary combination of amine and acid protecting groups together with nonfragment-based, total stepwise solid phase peptide synthesis led to the successful preparation of an analogue of the model peptide, insulin-like peptide 3 (INSL3), in which both of the interchain disulfide bonds were replaced with a lactam bond. An analogue containing a single disulfide-substituted interchain lactam bond was also prepared. Both INSL3 analogues retained significant cognate RXFP2 receptor binding affinity. PMID:24288548

  9. Total Chemical Synthesis of a Heterodimeric Interchain Bis-Lactam-Linked Peptide: Application to an Analogue of Human Insulin-Like Peptide 3

    Directory of Open Access Journals (Sweden)

    John Karas

    2013-01-01

    Full Text Available Nonreducible cystine isosteres represent important peptide design elements in that they can maintain a near-native tertiary conformation of the peptide while simultaneously extending the in vitro and in vivo half-life of the biomolecule. Examples of these cystine mimics include dicarba, diselenide, thioether, triazole, and lactam bridges. Each has unique physicochemical properties that impact upon the resulting peptide conformation. Each also requires specific conditions for its formation via chemical peptide synthesis protocols. While the preparation of peptides containing two lactam bonds within a peptide is technically possible and reported by others, to date there has been no report of the chemical synthesis of a heterodimeric peptide linked by two lactam bonds. To examine the feasibility of such an assembly, judicious use of a complementary combination of amine and acid protecting groups together with nonfragment-based, total stepwise solid phase peptide synthesis led to the successful preparation of an analogue of the model peptide, insulin-like peptide 3 (INSL3, in which both of the interchain disulfide bonds were replaced with a lactam bond. An analogue containing a single disulfide-substituted interchain lactam bond was also prepared. Both INSL3 analogues retained significant cognate RXFP2 receptor binding affinity.

  10. Réflexion sur l’origine du processus de segmentation du marche du travail

    Directory of Open Access Journals (Sweden)

    Attia Nicole

    2006-01-01

    Full Text Available (francuski Ce travail propose une réflexion sur l'origine du processus de segmentation du marché du travail par rapport à l'entreprise. Se situe-t-elle au sein même de l'entreprise ou en amont, c'est à dire entre les entreprises? Cela revient à se demander si on peut avoir une approche microéconomique ou macroéconomique de la segmentation et, à s'interroger sur le rôle réel tenu par les firmes dans le processus. Déterminant pour la théorie, ce rôle est à repenser selon la réponse apportée à notre question.

  11. Comparison of kinetic properties of amine oxidases from sainfoin and lentil and immunochemical characterization of copper/quinoprotein amine oxidases.

    Science.gov (United States)

    Zajoncová, L; Frébort, I; Luhová, L; Sebela, M; Galuszka, P; Pec, P

    1999-01-01

    Kinetic properties of novel amine oxidase isolated from sainfoin (Onobrychis viciifolia) were compared to those of typical plant amine oxidase (EC 1.4.3.6) from lentil (Lens culinaris). The amine oxidase from sainfoin was active toward substrates, such as 1,5-diaminopentane (cadaverine) with K(m) of 0.09 mM and 1,4-diaminobutane (putrescine) with K(m) of 0.24 mM. The maximum rate of oxidation for cadaverine at saturating concentration was 2.7 fold higher than that of putrescine. The amine oxidase from lentil had the maximum rate for putrescine comparable to the rate of sainfoin amine oxidase with the same substrate. Both amine oxidases, like other plant Cu-amine oxidases, were inhibited by substrate analogs (1,5-diamino-3-pentanone, 1,4-diamino-2-butanone and aminoguanidine), Cu2+ chelating agents (diethyltriamine, 1,10-phenanthroline, 8-hydroxyquinoline, 2,2'-bipyridyl, imidazole, sodium cyanide and sodium azide), some alkaloids (L-lobeline and cinchonine), some lathyrogens (beta-aminopropionitrile and aminoacetonitrile) and other inhibitors (benzamide oxime, acetone oxime, hydroxylamine and pargyline). Tested by Ouchterlony's double diffusion in agarose gel, polyclonal antibodies against the amine oxidase from sainfoin, pea and grass pea cross-reacted with amine oxidases from several other Fabaceae and from barley (Hordeum vulgare) of Poaceae, while amine oxidase from the filamentous fungus Aspergillus niger did not cross-react at all. However, using Western blotting after SDS-PAGE with rabbit polyclonal antibodies against the amine oxidase from Aspergillus niger, some degree of similarity of plant amine oxidases from sainfoin, pea, field pea, grass pea, fenugreek, common melilot, white sweetclover and Vicia panonica with the A. niger amine oxidase was confirmed. PMID:10092944

  12. Cri-du-chat syndrome

    NARCIS (Netherlands)

    Didden, H.C.M.; Curfs, L.M.G

    2013-01-01

    Cri-du-chat syndrome is a genetic disease resulting from a deletion occurring on the short arm of chromosome 5 (5p-). The incidence ranges from 1:15 000 to 1:50 000 live-born infants. Its main clinical features are a high-pitched monochromatic cry, microcephaly, broad nasal bridge, epicanthal folds,

  13. Biogenic amine metabolism in juvenile neurocardiogenic syncope with dysautonomia

    OpenAIRE

    Butler, Ian J.; Lankford, Jeremy E; Hashmi, Syed Shahrukh; Numan, Mohammed T

    2014-01-01

    Objective Biogenic amine brain levels and their cerebral metabolism are frequently studied by quantitation of biogenic amine metabolites in cerebrospinal fluid (CSF) compared to age-matched controls. There is a paucity of studies in adolescents and young adults investigating the potential role of disordered cerebral biogenic amine metabolism in young patients who have dysautonomia based on abnormal head-up tilt table (HUTT). Methods In a cohort of juvenile patients with neurocardiogenic synco...

  14. Characterization of a novel amine transaminase from Halomonas elongata

    OpenAIRE

    Cerioli, Lorenzo; Planchestainer, Matteo; Cassidy, Jennifer; Tessaro, Davide; Paradisi, Francesca

    2015-01-01

    Chiral amines are indispensable building blocks in the production of biologically active compounds. They are fundamental for the pharmaceutical industry, both as active molecules themselves and as chiral auxiliaries in asymmetric synthesis; however, the available synthetic strategies often present disadvantages. ω-Transaminases (ω-TAs) appear as an attractive alternative by driving the stereoselective amination of prochiral ketones. HEWT is a novel amine transaminase from the moderate halophi...

  15. Le corps du sultan ottoman

    Directory of Open Access Journals (Sweden)

    Nicolas Vatin

    2006-11-01

    Full Text Available La personne du sultan est la clef de voûte de l’État ottoman. C’est son existence qui assure le bon ordre du monde. Il n’est donc pas étonnant que la société ottomane n’ait jamais admis la possibilité d’une solution de continuité dynastique et ait, de ce fait, accordé une importance considérable à la présence physique de la personne du souverain. Ainsi le corps vivant du sultan, s’il n’était pas à proprement parler sacré, était cependant d’une nature surhumaine, d’une valeur supérieure qui était plus que symbolique et dépassait sa personne individuelle.En conséquence, mort ou déposé, le sultan n’était plus rien et aucun respect particulier n’était dû à sa personne physique. Bien au contraire, alors que, en cas de décès, il devenait en théorie un musulman comme un autre, sa qualité d’ancien souverain faisait qu’il avait moins de droits qu’un autre. Son corps pouvait subir des traitements plus ou moins dégradants dictés par des considérations politiques, à commencer par le refus de toute solution de continuité dynastique qui obligea plus d’une fois à maintenir le décès secret et à retarder de plusieurs semaines l’inhumation du défunt.On peut cependant discerner des éléments de sacralisation du corps mort du sultan, voire même les traces d’un culte qui aurait pu se développer. Mais celui-ci était sans doute trop païen pour s’imposer dans un État qui tendit au cours des siècles à renforcer l’orthodoxie de ses pratiques et à accorder une place croissante à la dynastie au détriment de la personne des sultans individuels.

  16. A regenerable solid amine CO2 concentrator for space station

    Science.gov (United States)

    Boehm, A. M.; Cusick, R. J.

    1982-01-01

    A regenerable solid amine CO2 control system, which employs water vapor for desorption, is being developed for potential use on long duration space missions. During cyclic operation, CO2 is first absorbed from the cabin atmosphere onto the granular amine. Steam is then used to heat the solid amine bed and desorb the CO2. This paper describes the solid amine system operation and application to the Shuttle Orbiter, Manned Space Platform (MSP) and Space Operations Center (SOC). The importance and interplay of system performance parameters are presented together with supporting data and design characteristics.

  17. Design, synthesis, and evaluation of chitosan conjugated GGRGDSK peptides as a cancer cell-targeting molecular transporter.

    Science.gov (United States)

    El-Sayed, Naglaa S; Shirazi, Amir N; El-Meligy, Magda G; El-Ziaty, Ahmed K; Nagieb, Zenat A; Parang, Keykavous; Tiwari, Rakesh K

    2016-06-01

    Targeting cancer cells using integrin receptor is one of the promising targeting strategies in drug delivery. In this study, we conjugated an integrin-binding ligand (GGRGDSK) peptide to chitosan oligosaccharide (COS) using sulfo-SMCC as a bifunctional linker to afford COS-SMCC-GGRGDSK. The conjugated polymer was characterized by FT-IR, (1)H NMR, (13)C NMR, and SEM. COS-SMCC-GGRGDSK did not show cytotoxicity up to a concentration of 1mg/mL in the human leukemia cell line (CCRF-CEM). The conjugate was evaluated for its ability to enhance the cellular uptake of a cell-impermeable cargo (e.g., F'-G(pY)EEI phosphopeptide) in CCRF-CEM, and human ovarian carcinoma (SK-OV-3) cancer cell lines. Additionally, RGD modified and unmodified COS polymers were used to prepare nanoparticles by ionic gelation and showed particle size ranging from 187 to 338nm, and zeta potential of 12.2-18.3mV using dynamic light scattering. The efficiency of COS-NPs and COS-SMCC-RGDSK NPs was assayed for translocation of two synthetic cytotoxic agents ((2-(2-aminoethylamino)-4-(4-chlorophenyl)-6-(1H-indol-3-yl) nicotinonitrile (ACIN), and 2-(2-aminoethylamino)-6-(1H-indol-3-yl)-4-(4-methoxyphenyl)-nicotinonitrile (AMIN)) into CCRF-CEM and human prostate (DU-145) cancer cell lines. The results showed a dramatic reduction in the cell viability on their treatment with RGD targeted COS NPs in comparison to paclitaxel (PTX), free drug, and drug-loaded COS NPs. PMID:26976071

  18. A Novel Palladium-catalyzed Amination of Aryl Halides with Amines Using rac-P-Phos as the Ligand

    Institute of Scientific and Technical Information of China (English)

    CHEN, Huansheng; WANG, Quanrui; TAO, Fenggang

    2009-01-01

    An efficient palladium-catalyzed amination of aryl halides with amines, using rac-P-Phos as the ancillary ligand and Pd(OAc)2 as the palladium source is developed. The ligand and all of the synthetic intermediates are stable to air and moisture, allowing the easy handling. The catalyst system performed well for a large number of different substrate combinations in 80-110 ℃ to furnish aromatic amines in high yields.

  19. Etude de l'affaiblissement du comportement mecanique du pergelisol du au rechauffement climatique

    Science.gov (United States)

    Buteau, Sylvie

    Le rechauffement climatique predit pour les prochaines decennies, aura des impacts majeurs sur le pergelisol qui sont tres peu documentes pour l'instant. La presente etude a pour but d'evaluer ces impacts sur les proprietes mecaniques du pergelisol et sa stabilite a long terme. Une nouvelle technique d'essai de penetration au cone a taux de deformation controle, a ete developpee pour caracteriser en place le pergelisol. Ces essais geotechniques et la mesure de differentes proprietes physiques ont ete effectues sur une butte de pergelisol au cours du printemps 2000. Le developpement et l'utilisation d'un modele geothermique 1D tenant compte de la thermodependance du comportement mecanique ont permis d'evaluer que les etendues de pergelisol chaud deviendraient instables a la suite d'un rechauffement de l'ordre de 5°C sur cent ans. En effet, la resistance mecanique du pergelisol diminuera alors rapidement jusqu'a 11,6 MPa, ce qui correspond a une perte relative de 98% de la resistance par rapport a un scenario sans rechauffement.

  20. LES GITES PLOMBO-ZINCIFERES DU DISTRICT MINIER DU KOUDIAT SIDU AUSUD DU KEF EN TUNISIE

    Directory of Open Access Journals (Sweden)

    Ivan Jurković

    1992-12-01

    Full Text Available Les auteurs ont dćcrit l:i stratigiaphie. I.i tectonique, le corps de minerals, la paragenese et la genese dcs gttes Koudiat Sidri situ&s environ 20 km au sud de la ville du Kef en Tunisie.

  1. Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s

    NARCIS (Netherlands)

    Hujaya, S.D.; Engbersen, J.F.J.; Paulusse, J.M.J.

    2015-01-01

    Purpose To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells. Methods Copolymers of phenylboronic acid-

  2. Amine reactivity with charged sulfuric acid clusters

    Directory of Open Access Journals (Sweden)

    B. R. Bzdek

    2011-08-01

    Full Text Available The distribution of charged species produced by electrospray of an ammonium sulfate solution in both positive and negative polarities is examined using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS. Positively-charged ammonium bisulfate cluster composition differs significantly from negatively-charged cluster composition. For positively-charged clusters all sulfuric acid is neutralized to bisulfate, whereas for negatively-charged clusters the degree of sulfuric acid neutralization is cluster size-dependent. With increasing cluster size (and, therefore, a decreasing role of charge, both positively- and negatively-charged cluster compositions converge toward ammonium bisulfate. The reactivity of negatively-charged sulfuric acid-ammonia clusters with dimethylamine and ammonia is also investigated by FTICR-MS. Two series of negatively-charged clusters are investigated: [(HSO4(H2SO4x] and [(NH4x(HSO4x+1(H2SO43]. Dimethylamine substitution for ammonia in [(NH4 x(HSO4 x+1(H2SO43] clusters is nearly collision-limited, and subsequent addition of dimethylamine to neutralize H2SO4 to bisulfate is within one order of magnitude of the substitution rate. Dimethylamine addition to [(HSO4 (H2SO4 x] clusters is either not observed or very slow. The results of this study indicate that amine chemistry will be evident and important only in large ambient negative ions (>m/z 400, whereas amine chemistry may be evident in small ambient positive ions. Addition of ammonia to unneutralized clusters occurs at a rate that is ~2–3 orders of magnitude slower than incorporation of dimethylamine either by substitution or addition

  3. The Multiple Facets of Iodine(III) Compounds in an Unprecedented Catalytic Auto-amination for Chiral Amine Synthesis.

    Science.gov (United States)

    Buendia, Julien; Grelier, Gwendal; Darses, Benjamin; Jarvis, Amanda G; Taran, Frédéric; Dauban, Philippe

    2016-06-20

    Iodine(III) reagents are used in catalytic one-pot reactions, first as both oxidants and substrates, then as cross-coupling partners, to afford chiral polyfunctionalized amines. The strategy relies on an initial catalytic auto C(sp(3) )-H amination of the iodine(III) oxidant, which delivers an amine-derived iodine(I) product that is subsequently used in palladium-catalyzed cross-couplings to afford a variety of useful building blocks with high yields and excellent stereoselectivities. This study demonstrates the concept of self-amination of the hypervalent iodine reagents, which increases the value of the aryl moiety. PMID:27158802

  4. PeptideAtlas

    Data.gov (United States)

    U.S. Department of Health & Human Services — PeptideAtlas is a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments. Mass...

  5. Trace Amines and the Trace Amine-Associated Receptor 1: Pharmacology, Neurochemistry and Clinical Implications

    Directory of Open Access Journals (Sweden)

    Yue ePei

    2016-04-01

    Full Text Available Biogenic amines are a collection of endogenous molecules that play pivotal roles as neurotransmitters and hormones. In addition to the classical biogenic amines resulting from decarboxylation of aromatic acids, including dopamine (DA, norepinephrine, epinephrine, serotonin (5-HT and histamine, other biogenic amines, present at much lower concentrations in the central nervous system (CNS, and hence referred to as trace amines (TAs, are now recognized to play significant neurophysiological and behavioural functions. At the turn of the century, the discovery of the trace amine-associated receptor 1 (TAAR1, a phylogenetically conserved G protein-coupled receptor that is responsive to both TAs, such as β-phenylethylamine, octopamine and tyramine, and structurally-related amphetamines, unveiled mechanisms of action for TAs other than interference with aminergic pathways, laying the foundations for deciphering the functional significance of TAs and its mammalian CNS receptor, TAAR1. Although its molecular interactions and downstream targets have not been fully elucidated, TAAR1 activation triggers accumulation of intracellular cAMP, modulates PKA and PKC signalling and interferes with the β-arrestin2-dependent pathway via G protein-independent mechanisms. TAAR1 is uniquely positioned to exert direct control over DA and 5-HT neuronal firing and release, which has profound implications for understanding the pathophysiology of, and therefore designing more efficacious therapeutic interventions for, a range of neuropsychiatric disorders that involve aminergic dysregulation, including Parkinson’s disease, schizophrenia, mood disorders and addiction. Indeed, the recent development of novel pharmacological tools targeting TAAR1 has uncovered the remarkable potential of TAAR1-based medications as new generation pharmacotherapies in neuropsychiatry. This review summarizes recent developments in the study of TAs and TAAR1, their intricate neurochemistry and

  6. Trace Amines and the Trace Amine-Associated Receptor 1: Pharmacology, Neurochemistry, and Clinical Implications.

    Science.gov (United States)

    Pei, Yue; Asif-Malik, Aman; Canales, Juan J

    2016-01-01

    Biogenic amines are a collection of endogenous molecules that play pivotal roles as neurotransmitters and hormones. In addition to the "classical" biogenic amines resulting from decarboxylation of aromatic acids, including dopamine (DA), norepinephrine, epinephrine, serotonin (5-HT), and histamine, other biogenic amines, present at much lower concentrations in the central nervous system (CNS), and hence referred to as "trace" amines (TAs), are now recognized to play significant neurophysiological and behavioral functions. At the turn of the century, the discovery of the trace amine-associated receptor 1 (TAAR1), a phylogenetically conserved G protein-coupled receptor that is responsive to both TAs, such as β-phenylethylamine, octopamine, and tyramine, and structurally-related amphetamines, unveiled mechanisms of action for TAs other than interference with aminergic pathways, laying the foundations for deciphering the functional significance of TAs and its mammalian CNS receptor, TAAR1. Although, its molecular interactions and downstream targets have not been fully elucidated, TAAR1 activation triggers accumulation of intracellular cAMP, modulates PKA and PKC signaling and interferes with the β-arrestin2-dependent pathway via G protein-independent mechanisms. TAAR1 is uniquely positioned to exert direct control over DA and 5-HT neuronal firing and release, which has profound implications for understanding the pathophysiology of, and therefore designing more efficacious therapeutic interventions for, a range of neuropsychiatric disorders that involve aminergic dysregulation, including Parkinson's disease, schizophrenia, mood disorders, and addiction. Indeed, the recent development of novel pharmacological tools targeting TAAR1 has uncovered the remarkable potential of TAAR1-based medications as new generation pharmacotherapies in neuropsychiatry. This review summarizes recent developments in the study of TAs and TAAR1, their intricate neurochemistry and

  7. Le parcours migratoire de jeunes ruraux du bled du kif

    Directory of Open Access Journals (Sweden)

    Khalid Mouna

    2015-06-01

    Full Text Available Cet article analyse le parcours migratoire des jeunes ruraux originaires des zones de production du cannabis, jeunes qui cherchent à briser les chaînes de soumission et d’humiliation vécues au quotidien. Pour les jeunes concernés par notre étude, la migration constitue un moyen de s’intégrer dans des réseaux transnationaux et ainsi d’entamer une carrière de beznass (commerçant du cannabis. Ce parcours « initiatique » permet à ces jeunes de revenir au bled avec de nouvelles idées, des moyens accrus, et de jouer un rôle actif dans l’économie locale – qui reste pour eux focalisée sur la production de cannabis, cette dernière restant néanmoins officiellement interdite.

  8. Biofunctionalization of polycaprolactone scaffolds with RGD peptides for the better cells integration

    Science.gov (United States)

    Matveeva, V. G.; Seifalian, A. M.; Antonova, L. V.; Velikanova, E. A.; Sergeeva, E. A.; Krivkina, E. O.; Glushkova, T. V.; Kudryavtseva, Yu. A.; Barbarash, O. L.; Barbarash, L. S.

    2016-08-01

    Here we tested in vitro electrospun polycaprolactone (PCL) scaffolds carbodiimide linkage with RGD peptides and their unconjugated counterparts. The scaffolds possessed highly porous structure and were formed by randomly distributed fibers. Orange II staining and ninhydrin test confirmed successful amination of the PCL. For the assessment of cell adhesion, we colonized scaffolds with primary human fibroblasts and counted the number of alive and dead cells. After 6 days of incubation, the number of fibroblasts on the scaffolds modified by RGD peptides significantly exceeded the number on unmodified scaffolds; however, the distribution of the cells on functionalized scaffolds was uneven, possibly due to uneven distribution of RGD peptides. The percentage of dead cells on the scaffolds with RGD peptides was significantly lower compared to their unmodified counterparts. Therefore, conjugation of PCL scaffolds with RGD peptides improves their integration with cells. This can be used in regenerative medicine.

  9. PH dependent adhesive peptides

    Science.gov (United States)

    Tomich, John; Iwamoto, Takeo; Shen, Xinchun; Sun, Xiuzhi Susan

    2010-06-29

    A novel peptide adhesive motif is described that requires no receptor or cross-links to achieve maximal adhesive strength. Several peptides with different degrees of adhesive strength have been designed and synthesized using solid phase chemistries. All peptides contain a common hydrophobic core sequence flanked by positively or negatively charged amino acids sequences.

  10. Antimicrobial Peptides in 2014

    Directory of Open Access Journals (Sweden)

    Guangshun Wang

    2015-03-01

    Full Text Available This article highlights new members, novel mechanisms of action, new functions, and interesting applications of antimicrobial peptides reported in 2014. As of December 2014, over 100 new peptides were registered into the Antimicrobial Peptide Database, increasing the total number of entries to 2493. Unique antimicrobial peptides have been identified from marine bacteria, fungi, and plants. Environmental conditions clearly influence peptide activity or function. Human α-defensin HD-6 is only antimicrobial under reduced conditions. The pH-dependent oligomerization of human cathelicidin LL-37 is linked to double-stranded RNA delivery to endosomes, where the acidic pH triggers the dissociation of the peptide aggregate to release its cargo. Proline-rich peptides, previously known to bind to heat shock proteins, are shown to inhibit protein synthesis. A model antimicrobial peptide is demonstrated to have multiple hits on bacteria, including surface protein delocalization. While cell surface modification to decrease cationic peptide binding is a recognized resistance mechanism for pathogenic bacteria, it is also used as a survival strategy for commensal bacteria. The year 2014 also witnessed continued efforts in exploiting potential applications of antimicrobial peptides. We highlight 3D structure-based design of peptide antimicrobials and vaccines, surface coating, delivery systems, and microbial detection devices involving antimicrobial peptides. The 2014 results also support that combination therapy is preferred over monotherapy in treating biofilms.

  11. Peptide Nucleic Acid Synthons

    DEFF Research Database (Denmark)

    2004-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  12. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    2003-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  13. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    1998-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  14. Peptide Nucleic Acids (PNA)

    DEFF Research Database (Denmark)

    2002-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  15. Peptide-Carrier Conjugation

    DEFF Research Database (Denmark)

    Hansen, Paul Robert

    2015-01-01

    To produce antibodies against synthetic peptides it is necessary to couple them to a protein carrier. This chapter provides a nonspecialist overview of peptide-carrier conjugation. Furthermore, a protocol for coupling cysteine-containing peptides to bovine serum albumin is outlined....

  16. Reducing tube bundle deposition using alternative amines

    International Nuclear Information System (INIS)

    Particle deposition rates were measured in a high-temperature loop for magnetite and hematite depositing onto Inconel-600 under flow-boiling conditions with pH controlled, using one of the following amines: morpholine, ammonia, ethanolamine, or dimethylamine. Hematite particles deposited at rates 10 times greater than those measured for magnetite although the hematite deposition rate dropped when the loop was operated under reducing conditions. The magnetite deposition rate was influenced by the amine used to control the pH, with the relative rate decreasing in the following series: morpholine (1) : ethanolamine (0.72) : ammonia (0.51) dimethylamine (0.25). These trends in deposition rate are discussed in terms of the surface chemistry of the corrosion products. Deposition rates for both magnetite and hematite increased significantly once the mixture quality exceeded about 0.3, which may be related to a change in the heat-transfer mechanism from nucleate boiling to 2-phase forced-convection through a thin film. (author)

  17. Denitration of Uranyl Nitrate Using Tridodecyl Amine

    International Nuclear Information System (INIS)

    Nitrate extraction from uranyl nitrate using extractant tridodecyl amine and paraffin has been carried out. The aim of this research was to prepare uranyl nitrate with low nitrate content (acid deficiency uranyl nitrate/ADUN). ADUN is a raw material for making kernels uranium oxide in a spherical from which cannot easily be broken/cracked. This ADUN was prepared by extracting nitric acid in uranyl nitrate solution with tridodecyl amine (TDA) and paraffin. Nitric acid in uranyl nitrate solution moved into organic phase due to the complex formation with TDA. The aqueous phase was ADUN, it was than analyzed its nitric and uranium contents using titration method. Tree variables were observed, i.e. uranium contents (80-125 g/l), process temperature (50-100 oC) and TDA/Nitrate molar ratio (0.5-1). Experiment results showed that optimum condition accurate at uranium content of 100 g/l, temperature extraction 60-70 oC and TDA to Nitrate molar ratio 0.75-0.80 with an efficiency of 77 %. (author)

  18. Thermodynamics of organic mixtures containing amines

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Juan Antonio [G.E.T.E.F. Dpto Termodinamica y Fisica Aplicada, Facultad de Ciencias, Universidad de Valladolid, Valladolid 47071 (Spain)]. E-mail: jagl@termo.uva.es; Mozo, Ismael [G.E.T.E.F. Dpto Termodinamica y Fisica Aplicada, Facultad de Ciencias, Universidad de Valladolid, Valladolid 47071 (Spain); Fuente, Isaias Garcia de la [G.E.T.E.F. Dpto Termodinamica y Fisica Aplicada, Facultad de Ciencias, Universidad de Valladolid, Valladolid 47071 (Spain); Cobos, Jose Carlos [G.E.T.E.F. Dpto Termodinamica y Fisica Aplicada, Facultad de Ciencias, Universidad de Valladolid, Valladolid 47071 (Spain)

    2006-02-01

    Binary mixtures containing pyridine (PY), or 2-methylpyridine (2MPY) or 3-methylpyridine (3MPY) or 4-methylpyridine (4MPY) and an organic solvent as benzene, toluene, alkane, or 1-alkanol are investigated in the framework of DISQUAC. The corresponding interaction parameters are reported. The model describes accurately a whole set of thermodynamic properties: vapor-liquid equilibria (VLE), liquid-liquid equilibria (LLE), solid-liquid equilibria (SLE), molar excess Gibbs energies (G{sup E}), molar excess enthalpies (H{sup E}), molar excess heat capacities at constant pressure (C{sub P}{sup E}) and the concentration-concentration structure factor (S{sub CC}(0)). It is remarkable that DISQUAC correctly predicts the W-shaped curve of the C{sub P}{sup E} of the pyridine + n-hexadecane system. The model can be applied successfully to mixtures with strong positive or negative deviations from the Raoult's law. DISQUAC improves the theoretical results from UNIFAC (Dortmund version). The replacement of pyridine by a methylpyridine leads to a weakening of the amine-amine interactions, ascribed to the steric effect caused by the methyl group attached to the aromatic ring. This explains that for a given solvent (alkane, 1-alkanol) H{sup E}(pyridine)>H{sup E}(methylpyridine)

  19. Biogenic amines in submicron marine aerosol (Invited)

    Science.gov (United States)

    Facchini, M.

    2010-12-01

    Ammonium salts of dimethyl and diethyl amine (DMA+ and DEA+) have been detected in size segregated marine samples collected in the North Atlantic over open ocean and at a coastal site. DMA+ and DEA+ peak in the accumulation mode range while very low concentration, close to detection limit, are observed in the coarse size fractions, as well as in sea spray aerosol artificially generated in the laboratory using sea water. These results indicate a secondary formation pathway. DMA+ and DEA+ represent up to 20% of secondary organic aerosol (SOA) in our samples , and to our knowledge they are the most abundant organic species besides MSA ever detected in clean marine aerosol . Maximum concentrations have been observed during spring and summer when the biological activity is high and in clean marine air masses, thus indicating biogenic sources. Total organic nitrogen (ON) concentration also peaks in the accumulation mode range and represents in our samples a fraction from 32 to 54 % of the total SOA. Ammonium salt formation from biogenic amines might be an important source of marine SOA and atmospheric nitrogen at the global scale with a seasonal variation connected to the oceanic biological productivity and an atmospheric cycle parallel to that of the organosulfur species.

  20. Reducing tube bundle deposition with alternative amines

    International Nuclear Information System (INIS)

    Particle deposition rates have been measured in a high-temperature loop for magnetite and hematite depositing onto Inconel-600 under flow-boiling conditions with pH controlled using one of the following amines: morpholine, ammonia, ethanolamine, or dimethylamine. Hematite particles deposited at rates an order of magnitude greater than those measured for magnetite, although the hematite deposition rate dropped when the loop was operated under reducing conditions. The magnetite deposition rate was influenced by the amine used to control the pH, with the relative rate decreasing in the following series: morpholine (1) : ethanolamine (0.72) ammonia (0.51) : dimethylamine (0.25). These trends in deposition rate are discussed in terms of the surface chemistry of the corrosion products. Deposition rates for both magnetite and hematite increased significantly once the mixture quality exceeded about 0.3, which may be related to a change in the heat transfer mechanism from nucleate boiling to two-phase forced convection through a thin film. (author)

  1. Compte rendu du colloque : « Anthropologie du Maroc et du Maghreb »

    OpenAIRE

    Planeix, Ariel

    2015-01-01

    Du 8 au 10 septembre 2013 a eu lieu, à Essaouira (Maroc), le colloque « Anthropologie du Maroc et du Maghreb », troisième du genre après ceux de Tanger en 2003 et de Fès en 2007, organisé par le Centre Jacques Berque (CGB) sous l’impulsion de Baudouin Dupret, son directeur, et de Cédric Baylocq, alors postdoctorant et depuis enseignant à l’université Mundiapolis de Casablanca. L’ambition, derrière un tel intitulé, fut de convier une part représentative des travaux contemporains sur le Maroc e...

  2. Le paradigme du don face aux nouvelles réalités du monde du travail

    OpenAIRE

    Caillé, Alain

    2012-01-01

    Alain Caillé resitue le paradigme du don que le Mouvement anti-utilitariste en sciences sociales (MAUSS) s’emploie à dégager depuis une trentaine d’années avant de montrer comment les lunettes du don permettent de mieux voir ce qu’il y a dans la boîte noire des organisations et de mieux lire par là même les ravages du néomanagement. À cette contribution répond celle de Norbert Alter dans le cadre du dossier Chorus « Peut-on penser le travail par le don ? »

  3. Processing method for drainage-containing ethanol amine

    International Nuclear Information System (INIS)

    Ethanol amine is decomposed by aerobically acting Pseudomonas sp. When drainage contains hydrazine, copper sulfate and hydrogen peroxide are added to the drainage under exposed condition to remove hydrazine as a pretreatment of the decomposing step. With such procedures, ethanol amine in the drainage can be processed efficiently. (T.M.)

  4. Determination of Biogenic Amines with HPLC-APCI-MS

    Science.gov (United States)

    Determination of biogenic amines in fish samples can be used as a quality attribute and are commonly performed using a derivatization step followed by high pressure liquid chromatography (HPLC) and UV detection. Over estimation and misidentification of biogenic amines can occur when interfering comp...

  5. Synthesis of amine functionalized cellulose nanocrystals: optimization and characterization.

    Science.gov (United States)

    Akhlaghi, Seyedeh Parinaz; Zaman, Masuduz; Mohammed, Nishil; Brinatti, César; Batmaz, Rasim; Berry, Richard; Loh, Watson; Tam, Kam Chiu

    2015-05-29

    A simple protocol was used to prepare amine functionalized cellulose nanocrystals (CNC-NH2). In the first step, epichlorohydrin (EPH) was reacted with ammonium hydroxide to produce 2-hydroxy-3-chloro propylamine (HCPA). In the next step, HCPA was grafted to CNC using the etherification reaction in an organic solution media. Various reaction parameters, such as time, temperature, and reactant molar ratio were performed to determine the optimal reaction conditions. The final product (CNC-NH2(T)) was dialyzed for a week. Further purification via centrifugation yielded the sediment (CNC-NH2(P)) and supernatant (POLY-NH2). The presence of amine groups on the surface of modified CNC was confirmed by FTIR and the amine content was determined by potentiometric titration and elemental analysis. A high amine content of 2.2 and 0.6 mmol amine/g was achieved for CNC-NH2(T) and CNC-NH2(P), respectively. Zeta potential measurements confirmed the charge reversal of amine CNC from positive to negative when the pH was increased from 3 to 10. The flocculation of amine functionalized CNC due to its interactions with a negatively charged surfactant namely, sodium dodecyl sulfate (SDS) was investigated at pH 4. It showed promising results for applications, such as in flocculation of fine dispersions in water treatment. This simple and versatile synthetic method to produce high amine content CNC can be used for further conjugation as required for various applications. PMID:25933198

  6. ELPI+ measurements of aerosol growth in an amine absorption column

    NARCIS (Netherlands)

    Mertens, J.; Brachert, L.; Desagher, D.; Thielens, M.L.; Khakharia, P.M.; Goetheer, E.L.V.; Schaber, K.

    2014-01-01

    Recently, studies have appeared pointing out that aerosols can dominate the total amine emission from amine based PCCC pilot plant scale installations. For the design of countermeasure types (upstream or downstream of the PCCC installation), it is crucial to have an idea of the aerosol size distribu

  7. Secondary organic aerosol formation of primary, secondary and tertiary Amines

    Science.gov (United States)

    Amines have been widely identified in ambient aerosol in both urban and rural environments and they are potential precursors for formation of nitrogen-containing secondary organic aerosols (SOA). However, the role of amines in SOA formation has not been well studied. In this wrok, we use UC-Riversid...

  8. Analyse du discours et archive

    OpenAIRE

    Maingueneau, Dominique

    2007-01-01

    Les recherches qui se réclament de "l’analyse du discours" connaissent un développement considérable dans le monde entier ; en revanche, "l’école française d’analyse du discours" (AD) traverse une crise d’identité depuis le début des années 80. Dans cet exposé nous voudrions explorer les raisons de cette crise, puis préciser le concept d’archive qui, à notre sens, permet de prolonger la voie ouverte à la fin des années 1960. Mais il ne s’agit que d’une des voies possibles, dès lors que, comme...

  9. L’Harmonie du monde

    Directory of Open Access Journals (Sweden)

    Martine Clouzot

    2003-11-01

    Full Text Available La Bourgogne est particulièrement bien présente et représentée dans l’exposition sur la musique et ses représentations au Moyen Âge organisée par Isabelle Marchesin (université de Poitiers, Christine Laloue (conservatrice du Patrimoine au Musée et Martine Clouzot (université de Bourgogne, au Musée de la Musique à Paris du 26 mars au 27 juin 2004. En Côte-d’Or, à Dijon, la Bibliothèque municipale a donné son accord officiel pour le prêt de la Bible d’Etienne Harding, les Moralia in Job et u...

  10. L'energie du moustique

    CERN Document Server

    Augereau, J F

    2002-01-01

    ENSEMBLE DE QUATRE ARTICLES - LARGE HADRON COLLIDER: Le dernier accelerateur de particules du CERN, le LEP, produisait des faisceaux d'electrons de 100 GeV chacun. Le LHC, qui accelere des faisceaux de protons, leur communiquera une energie de 7 TeV chacun. Une energie a la fois colossale et derisoire. Un TeV represente a peu pres l'energie cinetique d'un moustique (1/2 page).

  11. Revision du Genre Aseraggodes Kaup

    NARCIS (Netherlands)

    Chabanaud, P.

    1930-01-01

    Le présent travail comprend la définition du genre Aseraggodes Kaup et la revision, sous forme d'une clef dichotomique, de toutes les espèces qui le composent, revision établie d'après les types eux-mêmes de ces espèces. Ce genre Aseraggodes appartient à la famille des Soleidae et à la sousfamille d

  12. Factors influencing biogenic amines accumulation in dairy products

    Directory of Open Access Journals (Sweden)

    Daniel M. eLinares

    2012-05-01

    Full Text Available Fermented foods are within the food products more often complained of having caused biogenic amines poisoning. Concerning milk-based fermented foods, cheese is the main product likely to contain significant levels of biogenic amines, specially tyramine, histamine and putrescine. Prompted by the increasing awareness of the risks related to dietary uptake of high biogenic amine loads, in this review we report about cheese elaboration and processing technological aspects affecting biogenic amines levels. Synthesis of biogenic amines is possible only when three conditions converge: i availability of the substrate amino acids; ii presence of microorganisms with the appropriate catabolic pathway activated; and iii environmental conditions favorable to the decarboxilation activity. These conditions depend on several factors such as milk treatment (pasteurization, use of starter cultures, NaCl concentration, time and temperature of ripening and preservation, pH… which will be discussed in this chapter.

  13. Amine Reactivity with Nanoclusters of Sulfuric Acid and Ammonia

    Science.gov (United States)

    Johnston, M. V.; Bzdek, B. R.; DePalma, J.

    2011-12-01

    Alkyl amines have emerged as key species in new particle formation and growth. This interest is reinforced by ambient measurements of amines (e.g. Smith et al., 2010) and enhanced levels of nitrogen (e.g. Bzdek et al., 2011) during growth of newly formed particles. An important mechanism of amine uptake is aminium salt formation, either by substituting for ammonium ions that already exist in the particle or by opening new channels for salt formation that are not favorable with ammonia. This presentation will focus on recent experimental and computational work in our group to study amine uptake into charged nanoclusters of sulfuric acid and ammonia. In the experimental work, clusters are produced by electrospray of an ammonium sulfate solution and then drawn into a Fourier transform ion cyclotron resonance mass spectrometer where a specific cluster is isolated and exposed to amine vapor. We find that amine reactivity is dependent on the size, composition and charge of the isolated cluster. For small clusters of either polarity, all ammonium ions reside on the surface and amine substitution occurs with near unit reaction probability. As the cluster size increases, an ammonium ion can be encapsulated in the center of the cluster, which provides a steric hindrance to amine substitution. Negatively charged clusters are more likely to be acidic than positively charged clusters. For acidic clusters, incoming amine molecules first substitute for preexisting ammonium ions and then add to the cluster until a "neutralized" aminium bisulfate composition is reached. Computational studies of these clusters provide fundamental insight into the thermodynamics and kinetics of amine uptake.

  14. Inauguration du C/R Forum (Belgique)

    OpenAIRE

    2009-01-01

    Le 16 février dernier, la Vlaamse beroepsvereniging Conservatie en Restauratie Forum (C/R Forum) a été constituée à l'initiative de 18 personnes du milieu de la conservation/restauration et du secteur de la protection du patrimoine. Etant donné que la conservation et la gestion du patrimoine culturel mobilier et immobilier de même que la formation et la recherche relèvent des communautés et des régions, la nécessité est apparue depuis un certain temps de créer une organisation Flamande au sei...

  15. Clefs du cœur, du corps et du texte

    OpenAIRE

    Pomel, Fabienne

    2016-01-01

    Glisser la clef d’amour dans la serrure du cœur, emporter ou détenir la clef du cœur : l’image se rencontre assez souvent dans les romans médiévaux. Il s’agira, à partir d’un corpus qui traite de l’amour profane – romans arthuriens, poésie lyrique, Roman de la Rose, jeux et dons courtois, traité intitulé La Clef d’amour –, de voir selon quelles modalités la métaphore de la clef est mobilisée pour dire l’amour comme expérience affective et sexuelle, mais aussi parfois le travail de la lectur...

  16. Etude du perçage et du soudage laser : dynamique du capillaire

    OpenAIRE

    Mostafa, Massaud

    2011-01-01

    L’objectif de ce travail est d'étudier expérimentalement la formation du capillaire durant le perçage et le soudage par faisceau laser, et de développer une simulation numérique permettant de reproduire la dynamique de formation et d'évolution du capillaire. Nous avons fait le choix d’utiliser comme matériau test le Zinc, en raison de ses propriétés thermodynamiques. Afin de simplifier le problème, nous avons étudié dans un premier temps le mécanisme de perçage. Deux méthodes expérimentales o...

  17. Au fil du temps (1976 ou la loi du seuil

    Directory of Open Access Journals (Sweden)

    Isabelle Singer

    2010-04-01

    Full Text Available Ayant choisi de vivre dans un camion, les héros d’Au fil du temps (film de Wim Wenders de 1976 font du seuil une expérience particulière. Celle de la rupture avec toute idée de foyer et celle du refus d’appartenance à la terre natale, cette terre allemande traversée par une frontière le long de laquelle ils vont voyager, et qui les renvoie à chaque instant aux traumatismes de l’Histoire. Sur le pare-brise du camion, l’extérieur (campagnes indifférenciées, villes à l’abandon… et l’intérieur se superposent. Au fil du temps questionne le paysage : il s’agit d’en décoller un à un les mythes qui le recouvrent. L’image alors n’est plus surface mais volume à traverser, à lacérer et découvrir ce qui est tissé dans le paysage. La démarche de Wenders est alors proche de celle d’un de ses contemporains : Anselm Kiefer. Le paysage allemand provoque le rejet parce qu’il y a là toujours plus que le visible : des strates et des strates de culpabilité que le mythe - et c’est sa fonction - a recouvert. Et qu’il s’agira ici, de soulever. Le choix du nomadisme, c’est celui d’un état de l’humanité antérieur à l’idée de patrie. Et c’est aussi celui de la solitude, comme prix à payer à ce refus d’appartenance et à cette mise à jour des mythes. L’appartenance à la terre allemande et à son Histoire est profondément problématique parce que les pères sont fondamentalement coupables. On se reconnaîtra alors des pères de substitution : des pères de cinéma (Nicholas Ray ou Fritz Lang. Et l’on substituera l’Histoire du cinéma à l’Histoire. Bruno est réparateur ambulant de projecteurs et Au fil du temps dressera, au gré de ses pérégrinations, un état des lieux du cinéma allemand des années soixante-dix : déliquescent, colonisé par les images hollywoodiennes. Il faut que cela change : état du cinéma ; état des protagonistes solitaires en quête d’une identit

  18. Thermodynamic Analysis of the Amin Engine

    Science.gov (United States)

    Wheeler, John C.

    2002-11-01

    Extraordinary claims for the The Amin engine, made recently in full-page paid advertisements in Science Magazine and Physics Today are based upon faulty analysis of the proposed cycle. As presented, the engine constitutes a claim to violate the second law of thermodynamics, purporting to produce net useful work while extracting heat from a reservoir at a single temperature. When analyzed correctly, it conforms to the second law and produces at most zero net work when operating under isothermal conditions. The author neglects the torque-angle work needed to change the angular velocity and/or moment of inertia of the working fluid in the cylinder. When this is correctly accounted for, the contradiction with the second law is resolved.

  19. N-(3-Methylphenylquinoxalin-2-amine monohydrate

    Directory of Open Access Journals (Sweden)

    Azila Idris

    2010-09-01

    Full Text Available The quinoxaline system in the title hydrate, C15H13N3·H2O, is roughly planar, the r.m.s. deviation for the 18 non-H atoms being 0.188 Å; this conformation features a short intramolecular C—H...N(pyrazine interaction. In the crystal, the amine H atom forms an N—H...O hydrogen bond to the water molecule, which in turn forms two O—H...N hydrogen bonds to the pyrazine N atoms of different organic molecules. These interactions lead to supramolecular arrays in the bc plane that are two molecules thick; additional π–π interactions stabilize the layers [ring centroid–centroid distance = 3.5923 (7 Å]. The layers stack along the a-axis direction via C—H...π contacts.

  20. Electrochemical characterization of aminated acrylic conducting polymer

    Energy Technology Data Exchange (ETDEWEB)

    Rashid, Norma Mohammad [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Lestari Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor Darul Ehsan (Malaysia); Heng, Lee Yook [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Lestari Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor Darul Ehsan (Malaysia); Southeast Asia Disaster Prevention Research Initiative, Lestari Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor Darul Ehsan (Malaysia); Ling, Tan Ling [Southeast Asia Disaster Prevention Research Initiative, Lestari Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor Darul Ehsan (Malaysia)

    2015-09-25

    New attempt has been made to synthesize aminated acrylic conducting polymer (AACP) using precursor of phenylvinylsulfoxide (PVS). The process was conducted via the integration of microemulsion and photopolymerization techniques. It has been utilized for covalent immobilization of amino groups by the adding of N-achryiloxisuccinimide (NAS). Thermal eliminating of benzene sulfenic acids from PVS has been done at 250 °C to form electroactive polyacetylene (PA) segment. Characterization of AACP has been conducted using fourier transform infrared (FTIR), scanning electron microscopy (SEM) and linear sweep cyclic voltammetry (CV). A range of 0.3-1.25μm particle size obtained from SEM characterization. A quasi-reversible system performed as shown in electrochemical study.

  1. 菲律宾蛤仔酶解多肽抗前列腺癌DU-145细胞的活性研究%Study on Antiproliferative Activity of Ruditapes philippinarum Hydrolysate on Human Prostate Cancer DU-145 Cell

    Institute of Scientific and Technical Information of China (English)

    李连军; 徐律; 杨最素; 丁国芳; 孙瑜

    2013-01-01

    [Objective] To study Philippines clam enzymolysis peptide on prostate cancer DU-145 cells proliferation activity in vitro.[Method] Digestive enzyme was used to enzymolysis the Philippine clam guts,3 kD molecular weight cut-off membranes were used to fractionate the Ruditapes philippinarum hydrolysate.The anti-proliferation activity and the early apoptosis of Ruditapes philippinarum peptides in vitro were detected by MTT,inverted microscope and AO/EB double staining respectively.[Result] Determined by MTT method,different concentration of peptides to DU-145 cells had obvious growth inhibition.Cell apoptosis morphological feature was observed under AO/EB double staining and inverted microscope.[Conclusion] Ruditapes philippinarum under 3 kD enzyme solution of molecular weight with inhibition of DU-145 cells growth,induce prostate cancer cells apoptosis,and a concentration and time dependence.%[目的]探讨菲律宾蛤仔酶解多肽抗人前列腺癌DU-145细胞增殖的活性.[方法]对菲律宾蛤仔内脏进行酶解,经超滤截取3 kD以下的多肽,应用MTT法、倒置显微镜观察和AO/EB染色等方法检测其体外抗DU-145细胞的活性.[结果]MTT法检测结果表明不同浓度的多肽对DU-145细胞有明显的生长抑制作用.通过倒置显微镜观察和AO/EB荧光双染观察到DU-145细胞出现细胞凋亡的形态学特征.[结论]菲律宾蛤仔3 kD以下的多肽具有抑制DU-145细胞生长,呈浓度和时间依赖性,并诱导细胞凋亡作用.

  2. Une approche comparative de la question de l'effectivité du droit du travail

    OpenAIRE

    Auvergnon, Philippe

    2006-01-01

    La question de l'effectivité du droit du travail n'est pas nouvelle. Toutefois, une approche comparative, à partir de différents systèmes juridiques et de relations professionnelles, permet utilement de la revisiter. Qu'entend-on par effectivité du droit ? Ne confond-on pas aujourd'hui souvent effectivité et efficacité ? Le droit du travail est-il particulièrement exposé à l'ineffectivité du fait des rapports de pouvoir prévalant dans les relations de travail subordonné ? L'analyse des effect...

  3. Poly(Amido Amine)s Containing Agmatine and Butanol Side Chains as Efficient Gene Carriers.

    Science.gov (United States)

    Won, Young-Wook; Ankoné, Marc; Engbersen, Johan F J; Feijen, Jan; Kim, Sung Wan

    2016-04-01

    A new type of bioreducible poly(amido amine) copolymer is synthesized by the Michael addition polymerization of cystamine bisacrylamide (CBA) with 4-aminobutylguanidine (agmatine, AGM) and 4-aminobutanol (ABOL). Since the positively charged guanidinium groups of AGM and the hydroxybutyl groups of ABOL in the side chains have shown to improve the overall transfection efficiency of poly(amido amine)s, it is hypothesized that poly(CBA-ABOL/AGM) synthesized at the optimal ratio of both components would result in high transfection efficiency and minimal toxicity. In this study, a series of the poly(CBA-ABOL/AGM) copolymers is synthesized as gene carriers. The polymers are characterized and luciferase transfection efficiencies of the polymers in various cell lines are investigated to select the ideal ratio between AGM and ABOL. The poly(CBA-ABOL/AGM) containing 80% AGM and 20% ABOL has shown the best transfection efficiency with the lowest cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier. PMID:26663734

  4. The sugar model: catalysis by amines and amino acid products

    Science.gov (United States)

    Weber, A. L.

    2001-01-01

    Ammonia and amines (including amino acids) were shown to catalyze the formation of sugars from formaldehyde and glycolaldehyde, and the subsequent conversion of sugars to carbonylcontaining products under the conditions studied (pH 5.5 and 50 degrees C). Sterically unhindered primary amines were better catalysts than ammonia, secondary amines, and sterically hindered primary amines (i.e. alpha-aminoisobutyric acid). Reactions catalyzed by primary amines initially consumed formaldehyde and glycolaldehyde about 15-20 times faster than an uncatalyzed control reaction. The amine-catalyzed reactions yielded aldotriose (glyceraldehyde), ketotriose (dihydroxyacetone), aldotetroses (erythrose and threose), ketotetrose (erythrulose), pyruvaldehyde, acetaldehyde, glyoxal, pyruvate, glyoxylate, and several unindentified carbonyl products. The concentrations of the carbonyl products, except pyruvate and ketotetrose, initially increased and then declined during the reaction, indicating their ultimate conversion to other products (like larger sugars or pyruvate). The uncatalyzed control reaction yielded no pyruvate or glyoxylate, and only trace amounts of pyruvaldehyde, acetaldehyde and glyoxal. In the presence of 15 mM catalytic primary amine, such as alanine, the rates of triose and pyruvaldehyde of synthesis were about 15-times and 1200-times faster, respectively, than the uncatalyzed reaction. Since previous studies established that alanine is synthesized from glycolaldehyde and formaldehyde via pyruvaldehyde as its direct precursor, the demonstration that the alanine catalyzes the conversion of glycolaldehyde and formaldehyde to pyruvaldehyde indicates that this synthetic pathway is capable of autocatalysis. The relevance of this synthetic process, named the Sugar Model, to the origin of life is discussed.

  5. Water-soluble primary amine compounds in rural continental precipitation

    Science.gov (United States)

    Gorzelska, Krystyna; Galloway, James N.; Watterson, Karen; Keene, William C.

    Procedures for collecting, storing and analysing precipitation samples for organic nitrogen studies were developed. These procedures preserve chemical integrities of the species of interest, allow for up to 3 months storage and quantitative determination of water-soluble primary amine compounds, with the overall error at the 2 nM detection limit of less than 30%. This methodology was applied to study amino compounds in precipitation samples collected over a period of one year in central Virginia. Nitrogen concentrations of 13 amino acids and 3 aliphatic amines were summed to calculate the total amine nitrogen (TAN). The concentration of TAN ranged from below our detection level to 6658 nM, and possibly reflected a seasonal variation in the source strength of the atmospheric amines. Overall, the most commonly occurring amino compounds were methyl amine, ethyl amine, glutamic acid, glycine and serine. On average, the highest overall contribution to the TAN came from arginine, asparagine, glutamine, methyl amine, serine and alanine. However, large qualitative and quantitative variations observed among samples warrant caution in interpretation and application of the averaged values. TAN in Charlottesville precipitation contributed from less than 1 to ca 10% of the ammonium nitrogen level. However, our estimates show that amino compounds may contribute significantly to reduced nitrogen budget in precipitation in remote regions.

  6. Session du Conseil du CERN : le ministre britannique, Robert Jackson, souligne l'intérêt de on pays pour l'avenir du CERN : décisions du Conseil pour la mise en oeuvre des recommandations du Comité d'évaluation du CERN: départ anticipé pour 200 membres au moins du personnel - mise à jour de la méthode de calcule pour les contributions des Etats Membres au budget

    CERN Multimedia

    CERN Press Office. Geneva

    1988-01-01

    Session du Conseil du CERN : le ministre britannique, Robert Jackson, souligne l'intérêt de on pays pour l'avenir du CERN : décisions du Conseil pour la mise en oeuvre des recommandations du Comité d'évaluation du CERN: départ anticipé pour 200 membres au moins du personnel - mise à jour de la méthode de calcule pour les contributions des Etats Membres au budget

  7. Armadillo : Grotte en bordure du rio Armadillo

    OpenAIRE

    Michelet, Dominique

    1981-01-01

    Près d'Armadillo, municipio Armadillo, San Luis Potosi.Cueva pricta. Grotte en bordure du rio Armadillo, dans les laves. Le sol de la grotte est à environ 3 mètres au dessus du lit de la rivière.Photo prise vers le Nord

  8. Des Taiwanais dans Ie sud du Fujian

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Se trouvant dans le sud-est de la Chine au bord dela mer, la province du Fujian s’appelle aussi toutsimplement "Min". Les anc(?)tres d’une grande partie de lapopulation de Taiwan sont venus du Fujian (Min). Les deux

  9. BIOGENIC AMINES CONTENT IN SELECTED WINES DURING WINEMAKING

    Directory of Open Access Journals (Sweden)

    Radka Flasarová

    2012-02-01

    Full Text Available The aim of this study was to describe the development of selected biogenic amines (histamine; tyramine; phenylethylamine; putrescine; agmatine; and cadaverine during the winemaking in 10 selected species grown in Central Europe in 2008. The analysis was performed using ion-exchange chromatography by the sodium-citrate buffers with the post-column ninhydrin derivatization and photometric detection. A comparison of the content of biogenic amines in red and wine varieties showed that red wines have higher concentrations of biogenic amines.

  10. Plant signalling peptides

    OpenAIRE

    Wiśniewska, Justyna; Trejgell, Alina; Tretyn, Andrzej

    2003-01-01

    Biochemical and genetic studies have identified peptides that play crucial roles in plant growth and development, including defence mechanisms in response to wounding by pests, the control of cell division and expansion, and pollen self-incompatibility. The first two signalling peptides to be described in plants were tomato systemin and phytosulfokine (PSK). There is also biochemical evidence that natriuretic peptide-like molecules, immunologically-relatedt o those found ...

  11. L'imaginaire du volcan

    OpenAIRE

    Bertrand, Dominique; Bosquet, Marie-Françoise; Bozzetto, Roger; Chamart, Gabrielle; Chelebourg, Christian; Chenet-Faugeras, Françoise; Collot, Michel; Cornille, Jean-Louis; Gaillard, Aurélia; Lavocat, Françoise; Frank LESTRINGANT; Racault, Jean-Michel; SHINODA, Chiwaki; Sylvos, Françoise; Tardieu, Jean-Pierre

    2016-01-01

    Quelle force naturelle pouvait, mieux que le volcan, devenir la métaphore vive de l'enthousiasme poétique ? Auteur du paysage qu'il remodèle après l'avoir détruit, sculpteur de laves autant qu'objet pittoresque, le volcan est dans la littérature un actant essentiel, un relais de l'auteur, comme le montre ce voyage dans la mémoire des représentations volcaniques.

  12. Environnement climatique du Pacifique Sud

    OpenAIRE

    Merle, Jacques

    1995-01-01

    L'environnement climatique du Pacifique Sud est conditionné par "l'extrême océanité" de la région et l'interaction étroite entre l'atmosphère et l'océan qui conditionne la vie et l'ensemble des paramètres environnementaux des pays insulaires. Les deux principaux phénomènes climatiques qui affectent la région sont : ENSO (El Nino and Southern Oscillation) et le réchauffement global. Ces deux signaux climatiques, facteurs principaux de la variabilité climatique, font sentir leurs actions sur l'...

  13. Mali. Musique bambara du Baninko

    OpenAIRE

    Zanetti, Vincent

    2011-01-01

    Dans le Mali contemporain, le groupe ethnique le plus important numériquement est celui que la littérature ethnographique a définitivement contribué à faire connaître sous le nom de «Bambara». Installés sur un vaste territoire recouvrant grosso modo le quart sud-est du pays, les Bambara, qui dans leur propre langue se désignent en fait sous le nom de bamananw (au singulier: bamanan), se distinguent les uns les autres selon leur répartition géographique, dans les trois régions suivantes: Beled...

  14. Les controverses du bien mourir

    OpenAIRE

    Clapasson, Dominique; Pott, Murielle

    2009-01-01

    Actuellement, la question des situations de fin de vie se pose en problème social. La dénonciation du tabou de la mort et de l’acharnement thérapeutique suscite des interrogations éthiques relatives à la souffrance des malades. Dès lors, le bien mourir devient une préoccupation qui touche chacun d’entre-nous. En Suisse, l’aspiration à une mort pacifiée se meut en deux modèles distincts de la gestion des situations de fin de vie : les soins palliatifs et l’assistance au suicide. En oppositi...

  15. Role of solution conformation and flexibility of short peptide ligands that bind to the p56(lck) SH2 domain

    NARCIS (Netherlands)

    Dekker, Frank J; de Mol, Nico J; Bultinck, Patrick; Kemmink, Johan; Hilbers, Hans W; Liskamp, Rob M J; Dekker, Frank

    2003-01-01

    A general approach in drug design is making ligands more rigid in order to avoid loss in conformational entropy (deltaS(conf)) upon receptor binding. We hypothesized that in the high affinity binding of pYEEI peptide ligands to the p56(lck) SH2 domain this loss in deltaS(conf) might be diminished du

  16. Polycyclic peptide therapeutics.

    Science.gov (United States)

    Baeriswyl, Vanessa; Heinis, Christian

    2013-03-01

    Owing to their excellent binding properties, high stability, and low off-target toxicity, polycyclic peptides are an attractive molecule format for the development of therapeutics. Currently, only a handful of polycyclic peptides are used in the clinic; examples include the antibiotic vancomycin, the anticancer drugs actinomycin D and romidepsin, and the analgesic agent ziconotide. All clinically used polycyclic peptide drugs are derived from natural sources, such as soil bacteria in the case of vancomycin, actinomycin D and romidepsin, or the venom of a fish-hunting coil snail in the case of ziconotide. Unfortunately, nature provides peptide macrocyclic ligands for only a small fraction of therapeutic targets. For the generation of ligands of targets of choice, researchers have inserted artificial binding sites into natural polycyclic peptide scaffolds, such as cystine knot proteins, using rational design or directed evolution approaches. More recently, large combinatorial libraries of genetically encoded bicyclic peptides have been generated de novo and screened by phage display. In this Minireview, the properties of existing polycyclic peptide drugs are discussed and related to their interesting molecular architectures. Furthermore, technologies that allow the development of unnatural polycyclic peptide ligands are discussed. Recent application of these technologies has generated promising results, suggesting that polycyclic peptide therapeutics could potentially be developed for a broad range of diseases. PMID:23355488

  17. An approach to the construction of tailor-made amphiphilic peptides that strongly and selectively bind to hairpin RNA targets.

    Science.gov (United States)

    Lee, Su Jin; Hyun, Soonsil; Kieft, Jeffrey S; Yu, Jaehoon

    2009-02-18

    The hairpin RNA motif is one of the most frequently observed secondary structures and is often targeted by therapeutic agents. An amphiphilic peptide with seven lysine and eight leucine residues and its derivatives were designed for use as ligands against RNA hairpin motifs. We hypothesized that variations in both the hydrophobic leucine-rich and hydrophilic lysine-rich spheres of these amphiphilic peptides would create extra attractive interactions with hairpin RNA targets. A series of alanine-scanned peptides were probed to identify the most influential lysine residues in the hydrophilic sphere. The binding affinities of these modified peptides with several hairpins, such as RRE, TAR from HIV, a short hairpin from IRES of HCV, and a hairpin from the 16S A-site stem from rRNA, were determined. Since the hairpin from IRES of HCV was the most susceptible to the initial series of alanine-scanned peptides, studies investigating how further variations in the peptides effect binding employed the IRES hairpin. Next, the important Lys residues were substituted by shorter chain amines, such as ornithine, to place the peptide deeper into the hairpin groove. In a few cases, a 70-fold improved binding was observed for peptides that contained the specifically located shorter amine side chains. To further explore changes in binding affinities brought about by alterations in the hydrophobic sphere, tryptophan residues were introduced in place of leucine. A few peptides with tryptophan in specific positions also displayed 70-fold improved binding affinities. Finally, double mutant peptides incorporating both specifically located shorter amine side chains in the hydrophilic region and tryptophan residues in the hydrophobic region were synthesized. The binding affinities of peptides containing the simple double modification were observed to be 80 times lower, and their binding specificities were increased 40-fold. The results of this effort provide important information about

  18. Biogenic amines in italian pecorino cheese.

    Science.gov (United States)

    Schirone, Maria; Tofalo, Rosanna; Visciano, Pierina; Corsetti, Aldo; Suzzi, Giovanna

    2012-01-01

    The quality of distinctive artisanal cheeses is closely associated with the territory of production and its traditions. Pedoclimatic characteristics, genetic autochthonous variations, and anthropic components create an environment so specific that it would be extremely difficult to reproduce elsewhere. Pecorino cheese is included in this sector of the market and is widely diffused in Italy (∼62.000t of production in 2010). Pecorino is a common name given to indicate Italian cheeses made exclusively from pure ewes' milk characterized by a high content of fat matter and it is mainly produced in the middle and south of Italy by traditional procedures from raw or pasteurized milk. The microbiota plays a major role in the development of the organoleptic characteristics of the cheese but it can also be responsible for the accumulation of undesirable substances, such as biogenic amines (BA). Bacterial amino acid decarboxylase activity and BA content have to be investigated within the complex microbial community of raw milk cheese for different cheese technologies. The results emphasize the necessity of controlling the indigenous bacterial population responsible for high production of BA and the use of competitive adjunct cultures could be suggested. Several factors can contribute to the qualitative and quantitative profiles of BA's in Pecorino cheese such as environmental hygienic conditions, pH, salt concentration, water activity, fat content, pasteurization of milk, decarboxylase microorganisms, starter cultures, temperature and time of ripening, storage, part of the cheese (core, edge), and the presence of cofactor (pyridoxal phosphate, availability of aminases and deaminases). In fact physico-chemical parameters seem to favor biogenic amine-positive microbiota; both of these environmental factors can easily be modulated, in order to control growth of undesirable microorganisms. Generally, the total content of BA's in Pecorino cheeses can range from about 100-2400

  19. Study on the Reaction of Benzimidazolium Salt with Amine Compounds

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The addition-hydrolysis reaction of benzimidazolium salt with some mono- andbifunctional amine nucleophiles is reported, and a novel method of biomimetic synthesis forformamides and heterocycle compounds is provided.

  20. Two simple amine hydrochlorides from the soft coral Lobophytum strictum

    Digital Repository Service at National Institute of Oceanography (India)

    Parameswaran, P.S.; Naik, C.G.; Das, B.; Kamat, S.Y.

    Two simple amine hydrochlorides, viz., 1-amino-1, 1-dimethyl-3-oxo-butane hydrochloride (1) (Diacetonamine) and 2, 2, 6, 6-tetramethylpiperidone hydrochloride (2) have been isolated from the fraction of the methanolic extract of the soft coral...

  1. FTIR Study of Enhanced Polymeric Blend Membrane with Amines

    Directory of Open Access Journals (Sweden)

    Asim Mushtaq

    2014-03-01

    Full Text Available In this study, research will be carried out to identify the functional group behavior of glassy and rubbery polymeric blend membrane with amines. Polymeric blend membranes with different blending ratios were prepared and the developed membranes were characterized by FTIR to see the effect of blend ratio on different functional groups. The developed membranes are flat dense sheet membrane of 20% wt/wt. The pure and blend membrane polysulfone, polyethersulfone, polyvinyl acetate with different composition, with 10% methyl diethanol amine, mono ethanol amine, diethanol amine are developed with dimethyl acetamide solvent. Fourier Transform Infrared (FTIR spectroscopy was utilized to study the interaction between two polymers and to analyze the type of bonding present. To observed frequencies were assigned to various mode of vibration in terms of fundamentals and combination. These spectral changes indicated the existence of molecular interaction among the enhanced polymeric blends; highlight the compatible nature among each other.

  2. Biogenic amines in meat and fermented meat products

    Directory of Open Access Journals (Sweden)

    Joanna Stadnik

    2010-09-01

    Full Text Available Recent trends in food quality and safety promote an increasing search for trace compounds that can affect human health. Biogenic amines belong to this group of substances. They can cause distinctive pharmacological, physiological and toxic effects in organisms. Their amounts are usually increasing as a consequence of the use of poor quality raw materials, during controlled or spontaneous microbial fermentation or in the course of food spoilage. The origin of biogenic amines makes them suitable as chemical indicators of the hygienic quality and freshness of some foods being associated to the degree of food fermentation or degradation. The development of appropriate manufacturing technologies to obtain products free or nearly free from biogenic amines is a challenge for the meat industry. This review briefly summarises current knowledge on the biological implications of biogenic amines on human health and collects data on the factors affecting their formation in meat and fermented meat products.

  3. Amine reclaiming technologies in post-combustion carbon dioxide capture.

    Science.gov (United States)

    Wang, Tielin; Hovland, Jon; Jens, Klaus J

    2015-01-01

    Amine scrubbing is the most developed technology for carbon dioxide (CO2) capture. Degradation of amine solvents due to the presence of high levels of oxygen and other impurities in flue gas causes increasing costs and deterioration in long term performance, and therefore purification of the solvents is needed to overcome these problems. This review presents the reclaiming of amine solvents used for post combustion CO2 capture (PCC). Thermal reclaiming, ion exchange, and electrodialysis, although principally developed for sour gas sweetening, have also been tested for CO2 capture from flue gas. The three technologies all have their strengths and weaknesses, and further development is needed to reduce energy usage and costs. An expected future trend for amine reclamation is to focus on process integration of the current reclaiming technologies into the PCC process in order to drive down costs.

  4. Amine reclaiming technologies in post-combustion carbon dioxide capture

    Institute of Scientific and Technical Information of China (English)

    Tielin Wang; Jon Hovland; KlauS J.Jens

    2015-01-01

    Amine scrubbing is the most developed technology for carbon dioxide (CO2) capture.Degradation of amine solvents due to the presence of high levels of oxygen and other impurities in flue gas causes increasing costs and deterioration in long term performance,and therefore purification of the solvents is needed to overcome these problems.This review presents the reclaiming of amine solvents used for post combustion CO2 capture (PCC).Thermal reclaiming,ion exchange,and electrodialysis,although principally developed for sour gas sweetening,have also been tested for CO2 capture from flue gas.The three technologies all have their strengths and weaknesses,and further development is needed to reduce energy usage and costs.An expected future trend for amine reclamation is to focus on process integration of the current reclaiming technologies into the PCC process in order to drive down costs.

  5. Sociologie et sociolinguistique du franbreu

    Directory of Open Access Journals (Sweden)

    Ben-Rafael Eliezer

    2014-07-01

    Full Text Available Des enquêtes ont montré que plus de 20 % des adultes israéliens ont quelque connaissance du français. Parmi cette population, 250 000 à 300 000 personnes pouvaient être considérées comme francophones. Le français a affirmé une présence importante – pas toujours reconnue – dès la phase cruciale du développement de cette société. Une immigration de masse suivit la création de l’État (1948 dont la population a quasiment triplé en moins de deux décennies, avec l’arrivée de plus de 200 000 immigrants d’Afrique du Nord et des dizaines de milliers des Balkans et du Moyen-Orient. Beaucoup parmi ces immigrants étaient francophones – le français étant pour eux soit la première langue soit une deuxième qu’ils contrôlaient. C’est depuis lors que la présence du français est devenue démographiquement significative dans la société israélienne, se prolongeant de nos jours dans l’immigration en provenance de France qui se poursuit jusqu’à aujourd’hui. Comme nous l’avons montré ailleurs (Ben-Rafael et Ben-Rafael 2013, Israël fait l’expérience simultanée de plusieurs francophonies originales qui diffèrent par leurs sources aussi bien que leurs expressions (Ben-Rafael et Ben-Rafael 2013. Un premier modèle est le « français vernaculaire » (voir Boudras-Chapon 2008 amené par les immigrants de classes défavorisées arrivant d’Afrique du nord qui se sont regroupés en Israël. Leur français n’a offert qu’une faible résistance à l’hébreu qui s’est imposée dans le processus d’intégration. Un bilinguisme fonctionnant sur un mode additif au début qui s’est mué progressivement en modèle soustractif. Un second modèle concerne les immigrants de même origine ainsi que des immigrants francophones d’autres pays (pays balkaniques, Turquie ou Egypte formant une strate instruite de classe moyenne, pour qui le français était d’utilisation quotidienne aussi bien que de langue de

  6. Two Dimensional Polyamides Prepared From Unsaturated Carboxylic Acids And Amines.

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, William F. (Utica, OH); Huang, Zhi Heng (East Lansing, MI); Wright, Stacy C. (Lansing, MI); Danzig, Morris (Northbrook, IL); Taylor, Andrew C. (Ann Arbor, MI)

    2002-07-17

    A polyamide and a process for preparing the polyamide are disclosed. The process comprises reacting in a reaction mixture a monomer selected from unsaturated carboxylic acids, esters of unsaturated carboxylic acids, anhydrides of unsaturated carboxylic acids, and mixtures thereof, and a first amine to form an intermediate reaction product in the reaction mixture, wherein the first amine is selected from RR.sub.1 NH, RNH.sub.2, RR.sub.1 NH.sub.2.sup.+, RNH.sub.3.sup.+ and mixtures thereof, wherein R and R.sub.1 can be the same or different and each contain between about 1 and 50 carbon atoms and are optionally substituted with heteroatoms oxygen, nitrogen, sulfur, and phosphorus and combinations thereof, and reacting the intermediate reaction product and a second amine to form a polyamide, wherein the second amine is selected from R.sub.2 R.sub.3 NH, R.sub.2 NH.sub.2, R.sub.2 R.sub.3 NH.sub.2.sup.+, R.sub.2 NH.sub.3.sup.+ and mixtures thereof wherein R.sub.2 and R.sub.3 can be the same or different and each contain between about 1 and 50 carbon atoms and are optionally substituted with heteroatoms oxygen, nitrogen, sulfur, and phosphorus and combinations thereof, wherein multiple of the R, R.sub.1, R.sub.2, and R.sub.3 are in vertically aligned spaced relationship along a backbone formed by the polyamide. In one version of the invention, the monomer is selected from maleic anhydride, maleic acid esters, and mixtures thereof. In another version of the invention, the first amine is an alkylamine, such as tetradecylamine, and the second amine is a polyalkylene polyamine, such as pentaethylenehexamine. In yet another version of the invention, the first amine and the second amine are olefinic or acetylenic amines, such as the reaction products of an alkyldiamine and an acetylenic carboxylic acid. The first amine and the second amine may be the same or different depending on the desired polyamide polymer structure.

  7. Volatile amines as criteria for chemical quality assessment. Seafoodplus Traceability

    OpenAIRE

    Etienne, Monique

    2005-01-01

    Volatile amines are the characteristic molecules responsible for the fishy odour and flavour present in fish several days after the catch and they are commonly used as criteria for assessing the fish quality. The nature and the formation of the volatile amines are discussed. Ammonia is present in freshly caught fish, during chilled storage it is formed by endogenous and bacterial enzymes, it is a poor indicator of fish freshness and cannot be considered as an effective marker of fish spoila...

  8. Factors Influencing Biogenic Amines Accumulation in Dairy Products

    OpenAIRE

    Linares, Daniel M.; del Río, Beatriz; Ladero, Victor; Martínez, Noelia; Fernández, María; Martín, María Cruz; Álvarez, Miguel A.

    2012-01-01

    Fermented foods are among the food products more often complained of having caused episodes of biogenic amines (BA) poisoning. Concerning milk-based fermented foods, cheese is the main product likely to contain potentially harmful levels of BA, specially tyramine, histamine, and putrescine. Prompted by the increasing awareness of the risks related to dietary uptake of high biogenic amine loads, in this review we report all those elaboration and processing technological aspects affecting BA bi...

  9. Factors influencing biogenic amines accumulation in dairy products

    OpenAIRE

    Alvarez, Miguel A.

    2012-01-01

    Fermented foods are among the food products more often complained of having caused episodes of biogenic amines (BA) poisoning. Concerning milk-based fermented foods, cheese is the main product likely to contain potentially harmful levels of BA, specially tyramine, histamine, and putrescine. Prompted by the increasing awareness of the risks related to dietary uptake of high biogenic amine loads, in this review we report all those elaboration and processing technological aspects affecting BA bi...

  10. Quantification of biogenic amines by microchip electrophoresis with chemiluminescence detection

    OpenAIRE

    Zhao, Shulin; Yong HUANG; Shi, Ming; Liu, Yi-Ming

    2009-01-01

    A highly sensitive microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of biogenic amines including agmatine, epinephrine, dopamine, tyramine, and histamine in human urine samples. To achieve a high assay sensitivity, the targeted analytes were pre-column labeled by a CL tagging reagent, N-(4-aminobutyl)-N-ethylisoluminol (ABEI). ABEI-tagged biogenic amines after MCE separation reacted with hydrogen peroxide in the presence of horse...

  11. Biogenic amines in meat and fermented meat products

    OpenAIRE

    Joanna Stadnik; Zbigniew J. Dolatowski

    2010-01-01

    Recent trends in food quality and safety promote an increasing search for trace compounds that can affect human health. Biogenic amines belong to this group of substances. They can cause distinctive pharmacological, physiological and toxic effects in organisms. Their amounts are usually increasing as a consequence of the use of poor quality raw materials, during controlled or spontaneous microbial fermentation or in the course of food spoilage. The origin of biogenic amines makes them suitabl...

  12. BIOGENIC AMINES CONTENT IN SELECTED WINES DURING WINEMAKING

    OpenAIRE

    Radka Flasarová; Leona Buňková; Barbora Ivičičová; František Buňka; Stanislav Kráčmar

    2012-01-01

    The aim of this study was to describe the development of selected biogenic amines (histamine; tyramine; phenylethylamine; putrescine; agmatine; and cadaverine) during the winemaking in 10 selected species grown in Central Europe in 2008. The analysis was performed using ion-exchange chromatography by the sodium-citrate buffers with the post-column ninhydrin derivatization and photometric detection. A comparison of the content of biogenic amines in red and wine varieties showed that red wines ...

  13. The analysis of biogenic amines by standard and novel methods

    OpenAIRE

    O'Sullivan, Brian

    2000-01-01

    Biologically active amines in cheese and fish arising from metabolic activities of food-borne microorganisms have been implicated as the causative agents in many food poisoning outbreaks. An awareness of amine levels in foods today is therefore important in relation to food spoilage and safety. In recent years there has been increased consumer awareness about food composition and safety and a corresponding increase in regulatory action. The food industry requires reliable and cost effecti...

  14. Aromatic amine dehydrogenase, a second tryptophan tryptophylquinone enzyme.

    OpenAIRE

    Govindaraj, S; Eisenstein, E.; Jones, L. H.; Sanders-Loehr, J; Chistoserdov, A Y; Davidson, V L; Edwards, S. L.

    1994-01-01

    Aromatic amine dehydrogenase (AADH) catalyzes the oxidative deamination of aromatic amines including tyramine and dopamine. AADH is structurally similar to methylamine dehydrogenase (MADH) and possesses the same tryptophan tryptophylquinone (TTQ) prosthetic group. AADH exhibits an alpha 2 beta 2 structure with subunit molecular weights of 39,000 and 18,000 and with a quinone covalently attached to each beta subunit. Neither subunit cross-reacted immunologically with antibodies to the correspo...

  15. Levels of histamine and other biogenic amines in high quality red wines.

    OpenAIRE

    Konakovsky, Viktor; Focke, Margarete; Hoffmann-Sommergruber, Karin; Schmid, Rainer,; Scheiner, Otto; Moser, Peter; Jarisch, Reinhart; Hemmer, Wolfgang

    2011-01-01

    Abstract Biogenic amines in wine may impair sensory wine quality and cause adverse health effects in susceptible individuals. In this study, histamine and other biogenic amines were determined by HPLC after amine derivatization to dansyl chloride conjugates in 100 selected high quality red wines made from seven different cultivars. Amine levels varied considerably between different wines. The most abundant amines were putrescine (median 19.4 mg/L, range 2.9-122), histamine (7.2, 0....

  16. Insulin C-peptide test

    Science.gov (United States)

    C-peptide ... the test depends on the reason for the C-peptide measurement. Ask your health care provider if ... C-peptide is measured to tell the difference between insulin the body produces and insulin someone injects ...

  17. Biogenic amines in wines from three Spanish regions.

    Science.gov (United States)

    Landete, José M; Ferrer, Sergi; Polo, Lucía; Pardo, Isabel

    2005-02-23

    One hundred and sixty-three wines from La Rioja, Utiel-Requena, and Tarragona were analyzed to determine if there were any differences in the concentrations of six biogenic amines that are found in these three regions. The influence of grape variety, type of vinification, wine pH, malolactic fermentation, and storage in bottle on biogenic amine concentrations was studied. Results show important differences in putrescine and histamine concentrations among regions, varieties of grape, and type of wine; differences were less appreciable for the remaining biogenic amines studied. Low pH prevented biogenic amine formation. Malolactic fermentation and short storage periods in bottle (3-6 months) showed increases in histamine concentration, whereas longer periods of storage led to a general decrease in histamine. Several strains of lactic acid bacteria were isolated in this work, and their ability to form biogenic amines was assayed in synthetic media, grape must, and wine. Grape varieties, different types of winemaking, pH, and lactic acid bacteria may be responsible for the differences observed in the biogenic amine concentrations of the wines analyzed.

  18. STUDIES ON VINYL POLYMERIZATION WITH INITIATION SYSTEM CONTAINING AMINE DERIVATIVES

    Institute of Scientific and Technical Information of China (English)

    QIU Kunyuan; ZHANG Jingyi; FENG Xinde(S. T. Voong)

    1983-01-01

    Two main types of amine-containing initiation systems were studied in this work. In the case of MMA polymerization initiated by BPO-amine (DMT, DHET, DMA) redox systems, it was found that the polymerization rate and colour stability of the polymer for different amine systems were in the following order: DMT≈DHET>DMA. Accordingly, BPO-DMT and BPO-DHET are effective initiators. In the case of MEMA polymerization by amine (DMT, DHET, DMA) alone, it was found that the polymerization rate and the percentage of conversion for these different amine systems were in the following order: DMT≥DHET>DMA. The polymerization rate and the percentage of conversion also increased with the increase of DMT concentration. From the kinetic investigation the rate equation of Rp=K [DMT]1/2 [MEMA]3/2 was obtained, and the overall activation energy of polymerization was calculated to be 34.3 KJ/mol (8.2 Kcal/mol). Moreover, the polymerization of MEMA in the presence of DMT was strongly inhibited by hydroquinone, indicating the polymerization being free radical in nature. From these results, the mechanism of MEMA polymerization initiated by amine was proposed.

  19. Amine-functionalized porous silicas as adsorbents for aldehyde abatement.

    Science.gov (United States)

    Nomura, Akihiro; Jones, Christopher W

    2013-06-26

    A series of aminopropyl-functionalized silicas containing of primary, secondary, or tertiary amines is fabricated via silane-grafting on mesoporous SBA-15 silica and the utility of each material in the adsorption of volatile aldehydes from air is systematically assessed. A particular emphasis is placed on low-molecular-weight aldehydes such as formaldehyde and acetaldehyde, which are highly problematic volatile organic compound (VOC) pollutants. The adsorption tests demonstrate that the aminosilica materials with primary amines most effectively adsorbed formaldehyde with an adsorption capacity of 1.4 mmolHCHO g(-1), whereas the aminosilica containing secondary amines showed lower adsorption capacity (0.80 mmolHCHO g(-1)) and the aminosilica containing tertiary amines adsorbed a negligible amount of formaldehyde. The primary amine containing silica also successfully abated higher aldehyde VOC pollutants, including acetaldehyde, hexanal, and benzaldehyde, by effectively adsorbing them. The adsorption mechanism is investigated by (13)C CP MAS solid-state NMR and FT-Raman spectroscopy, and it is demonstrated that the aldehydes are chemically attached to the surface of aminosilica in the form of imines and hemiaminals. The high aldehyde adsorption capacities of the primary aminosilicas in this study demonstrate the utility of amine-functionalized silica materials for reduction of gaseous aldehydes.

  20. Heterocyclic amines in meat and meat products

    Directory of Open Access Journals (Sweden)

    Aliye BULGAN

    2013-06-01

    Full Text Available Heterocyclic amines (HA are the mutagenic/carcinogenic compounds which generate as a result of cooking of red meat, poultry meat and fish fillets at high temperatures. Up to 20 different HAs were detected and classified in the researches that conducted on these types of meats cooked at high temperatures. HAs are the products of Maillard reactions and the Strecker degredation of main precursors such as creatine/creatinine, aminoacid and the polysaccharides. Many physical and chemical factors effect the formation of HAs. Thus, it was reported by many researchers that utilizing coating and marination processes in addition to using natural and synthetic antioxidants and seasonings-plant extracts were effective on inhibiting/decreasing the formation of HAs. Additionally, boiling/steaming and microwave cooking methodologies were recommended instead of barbecuing, grilling or frying to inhibit/decrease the formation of HAs. The HAs formed in meat and meat products and the factors which have effects on the formation of HAs are presented in this review.

  1. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    2004-01-01

    A novel class of compounds known as peptide nucleic acids, bind complementary DNA and RNA strands, and generally do so more strongly than the corresponding DNA or RNA strands while exhibiting increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from...

  2. COMMUNICATION DU CREDIT AGRICOLE - French version only

    CERN Multimedia

    2003-01-01

    La Direction du Crédit Agricole informe son aimable clientèle du CERN des jours et heures d'ouverture de l'agence du site de Prévessin à compter du mardi 14 janvier 2003 : 1. Horaires pour les opérations bancaires courantes 7 jours sur 7 et 24h/24 avec l'espace libre service bancaire. 2. Horaires conseil du mardi au vendredi - Mardi, de 9h. à 12h. et de 14h.15 à 16h.30. - Mercredi, jeudi et vendredi, de 9h. à 12h. et de 13h.30 à 16h.30. Deux collaboratrices au lieu d'une seront désormais présentes toute la journée du mardi au vendredi pour vous accueillir, vous informer et vous conseiller en crédits et placements (réception conseil sur rendez-vous). Autre nouveauté : les mêmes conseillers seront aussi à votre disposition le samedi, sur notre agence de Gex, de 8h.15 à 13h.05, notamment pour les études de financements habitat. La Direction et toute l'équipe de l'agence du Crédit Agricole vous souhaitent une excellent année 2003.

  3. Isolation of cross-linked peptides by diagonal strong cation exchange chromatography for protein complex topology studies by peptide fragment fingerprinting from large sequence databases.

    Science.gov (United States)

    Buncherd, Hansuk; Roseboom, Winfried; Ghavim, Behrad; Du, Weina; de Koning, Leo J; de Koster, Chris G; de Jong, Luitzen

    2014-06-27

    Knowledge of spatial proximity of amino acid residues obtained by chemical cross-linking and mass spectrometric analysis provides information about protein folding, protein-protein interactions and topology of macromolecular assemblies. We show that the use of bis(succinimidyl)-3-azidomethyl glutarate as a cross-linker provides a solution for two major analytical problems of cross-link mapping by peptide fragment fingerprinting (PFF) from complex sequence databases, i.e., low abundance of protease-generated target peptides and lack of knowledge of the masses of linked peptides. Tris(carboxyethyl)phosphine (TCEP) reduces the azido group in cross-linked peptides to an amine group in competition with cleavage of an amide bond formed in the cross-link reaction. TCEP-induced reaction products were separated by diagonal strong cation exchange (SCX) from unmodified peptides. The relation between the sum of the masses of the cleavage products and the mass of the parent cross-linked peptide enables determination of the masses of candidate linked peptides. By reversed phase LC-MS/MS analysis of secondary SCX fractions, we identified several intraprotein and interprotein cross-links in a HeLa cell nuclear extract, aided by software tools supporting PFF from the entire human sequence database. The data provide new information about interacting protein domains, among others from assemblies involved in splicing.

  4. De Sumer à Seattle, le voyage du bout du monde

    Directory of Open Access Journals (Sweden)

    Olivier Walther

    2008-12-01

    Full Text Available « Les affaires qui se nouent par-dessus les frontières qui séparent les peuples ne font pas que ces frontières n'existent pas » notait Émile Durkheim en 1895 (1895 : 113. Un peu plus d’un siècle plus tard, l’ouvrage de William J. Bernstein intitulé A Splendid Exchange et sous-titré de manière inspirée How Trade Shaped the World vient apporter une confirmation étayée au sociologue français. De Sumer où fut introduit le commerce du cuivre 3000 ans avant Jésus-Christ, à Seattle où se tint la tr...

  5. Le cas du Haut-Adige ou Tyrol du Sud

    Directory of Open Access Journals (Sweden)

    Licia Bagini Scantamburlo

    2007-06-01

    Full Text Available La situation de cette région italienne est vraiment emblématique des rapports entre « les territoires et l’identité » à cause de la présence de différentes communautés linguistiques autochtones, dont les plus importantes sont les minorités germanophone et latine. S’il est vrai que cette situation existe également dans d’autres régions italiennes, il est incontestable que dans le Haut-Adige/Tyrol du Sud elle présente des caractéristiques très spécifiques et uniques pour l’Italie, car les italo...

  6. De Sumer à Seattle, le voyage du bout du monde

    OpenAIRE

    Olivier Walther

    2011-01-01

    « Les affaires qui se nouent par-dessus les frontières qui séparent les peuples ne font pas que ces frontières n'existent pas » notait Émile Durkheim en 1895 (1895 : 113). Un peu plus d’un siècle plus tard, l’ouvrage de William J. Bernstein intitulé A Splendid Exchange et sous-titré de manière inspirée How Trade Shaped the World vient apporter une confirmation étayée au sociologue français. De Sumer où fut introduit le commerce du cuivre 3000 ans avant Jésus-Christ, à Seattle où se tint la tr...

  7. INDE. Le chant du Mohini Attam, danse classique du Kerala

    OpenAIRE

    Contri, Fabrice

    2014-01-01

    Voici un objet fort précieux, dans le meilleur sens du terme, comme ces guirlandes de joyaux dont sont ornées, en Inde, les divinités que ne cesse de célébrer le Mōhiniyāṭṭam ou « danse de l’Enchanteresse ». La dévotion avec tout l’élan de l’être – l’intellect, l’esprit, sans omettre le corps –, c’est bien un aspect essentiel de cette forme d’expression, ô combien subtile ; c’est également ce qui anime, avec ferveur, la danse et la musique indiennes. Ce nouveau Cd, de la très belle collection...

  8. Nouvelles du Centre Aéré de l’Association du Personnel du CERN

    CERN Multimedia

    Jardin d'enfants

    2015-01-01

    Cet été 2015, durant quatre semaines d’été, le Centre Aéré a accueilli plus de 40 enfants âgés de 4 à 6 ans. Devant le succès rencontré, et à la demande des parents, il a été décidé d’en doubler la capacité maximale. A l'été 2016, du 4 au 29 juillet, la structure pourra accueillir les enfants de 4 ans révolus et de moins de 7 ans (nés après le 31/07/2009 mais avant  01/07/2012). Les inscriptions se feront à la semaine durant le mois d'Avril 2016. Les programmes sont en cours d'élaboration cependant nous pouvons déjà vous communiquer le thème conducteur du centre qui sera : à la découverte d'un continent différent chaque ...

  9. L'impossible cartographie du terrorisme

    OpenAIRE

    Bigo, Didier

    2005-01-01

    Le terrorisme n'existe pas : ou plus exactement, ce n'est pas un concept utilisable par les sciences sociales et la stratégie. En revanche l'usage du terme a une forte signification politique. Pour expliquer ce paradoxe apparent, il est nécessaire d'analyser les usages du terme terrorisme et leurs implications. Nous qu'adversaires en présence comme tiers, victimes, font du terrorisme une forme de conflit spécifique qui aurait sa propre unité et qui pourrait être analysé selon une grille de le...

  10. Du glacier au glacier rocheux, depuis la fin du Petit Âge Glaciaire, au pied du Mont Thabor (Alpes du Nord, France)

    OpenAIRE

    Monnier, Sébastien

    2010-01-01

    Le glacier rocheux du Thabor-Cheval Blanc, à l’extrême sud de la Savoie (Alpes du Nord françaises), est un spécimen spectaculaire et fascinant. D’après les informations fournies par des documents historiques (cartes et croquis anciens), ce glacier rocheux s’est dévoilé dans le paysage au cours des deux derniers siècles : un vrai glacier se trouvait effectivement à son emplacement à la fin du Petit Âge Glaciaire. L’objectif de cet article est de comprendre comment a pu se produire une évolutio...

  11. Gênes et les réseaux du commerce du sucre à la fin du Moyen Âge

    OpenAIRE

    Ouerfelli, Mohamed

    2014-01-01

    La présente contribution examine le rôle des hommes d’affaires génois dans le commerce du sucre en Méditerranée, à la fin du Moyen Âge. S’ils montrent un désintérêt progressif pour ce trafic lucratif en Orient, notamment dans le royaume de Chypre, ils sont en revanche plus dynamiques en Sicile et surtout dans le royaume de Grenade. Dans cette dernière région, ils s’implantent solidement et obtiennent le monopole de l’exportation du sucre et des fruits secs, qu’ils acheminent jusqu’en Mer du N...

  12. Sorption of mono ethanol amine and mono ethanol aminate of copper(II) and cadmium(II) on hydrated zirconium dioxide

    International Nuclear Information System (INIS)

    The sorption of mono ethanol amine and mono ethanol aminate of copper(II) and cadmium(II) on hydrated zirconium dioxide is studied. Influence of ph of equilibrium solution, nature of central cation and outer-sphere anion on sorption of mono ethanol amine on hydrated zirconium dioxide is considered.

  13. Guanidinylated bioresponsive poly(amido amine)s designed for intranuclear gene delivery

    Science.gov (United States)

    Yu, Jiankun; Zhang, Jinmin; Xing, Haonan; Yang, Zhen; Cai, Cuifang; Zhang, Conglu; Zhao, Xiaoyun; Wei, Minjie; Yang, Li; Ding, Pingtian

    2016-01-01

    Guanidinylated poly(amido amine)s with multiple disulfide linkages (Gua-SS-PAAs) were designed and constructed as nonviral gene carriers. The main chains of these novel carriers were synthesized based on monomers containing guanidino groups (guanidine hydrochloride and chlorhexidine), which could avoid complicated side-chain-modification reactions while introducing the guanidino groups. The synthesized Gua-SS-PAAs polymers were characterized by 1H nuclear magnetic resonance, molecular weight, and polydispersity. Furthermore, Gua-SS-PAAs polymers were complexed with pDNA, and the properties of the complexes were determined, including entrapment efficiency, particle size, ζ-potential, atomic force microscopy images, stability, DNA complexation ability, reduction sensitivity, cytotoxicity, and transfection efficiency. The new Gua-SS-PAAs carriers exhibited higher transfection efficiency and lower cytotoxicity compared with two widely used gene delivery carriers, polyethylenimine and lipofectamine 2000. Furthermore, the relationship between the side-chain structure and morphological/biological properties was extrapolated, and the results showed that guanidine in the side chain aids in the improvement of transfection efficiency. In addition, the introduction of guanidino group might confer the new carriers with nuclear localization function compared to carriers without it. PMID:27574429

  14. Effets des contraintes mécaniques du sol sur la limitation des rendements du tournesol

    OpenAIRE

    Mirleau-Thebaud, Virginie

    2012-01-01

    La production de tournesol inclue des interactions complexes entre le génotype et l'environnement tout au long du cycle de la culture. La profondeur de l'enracinement du tournesol est fortement liée au sol, à sa structure et à la disponibilité en eau. La compaction du sol représente un enjeu important dans le contexte actuel de la durabilité des systèmes agricoles, et se caractérise par une diminution de la disponibilité hydrique du sol, une augmentation de la densité apparente et la résistan...

  15. Irritable bowel syndrome: the role of gut neuroendocrine peptides.

    Science.gov (United States)

    El-Salhy, Magdy; Seim, Inge; Chopin, Lisa; Gundersen, Doris; Hatlebakk, Jan Gunnar; Hausken, Trygve

    2012-01-01

    Irritable bowel syndrome (IBS) is a common chronic disorder with a prevalence ranging from 5 to 10 percent of the world's population. This condition is characterised by abdominal discomfort or pain, altered bowel habits, and often bloating and abdominal distension. IBS reduces quality of life in the same degree of impairment as major chronic diseases such as congestive heart failure and diabetes and the economic burden on the health care system and society is high. Abnormalities have been reported in the neuroendocrine peptides/amines of the stomach, small- and large intestine in patients with IBS. These abnormalities would cause disturbances in digestion, gastrointestinal motility and visceral hypersensitivity, which have been reported in patients with IBS. These abnormalities seem to contribute to the symptom development and appear to play a central role in the pathogenesis of IBS. Neuroendocrine peptides/amines are potential tools in the treatment and diagnosis of IBS. In particular, the cell density of duodenal chromogranin A expressing cells appears to be a good histopathological marker for the diagnosis of IBS with high sensitivity and specificity. PMID:22652678

  16. Amine-pillared Nanosheet Adsorbents for CO2 Capture Applications

    Science.gov (United States)

    Jiang, Hui

    Amine-functionalized solid adsorbents have gained attention within the last decade for their application in carbon dioxide capture, due to their many advantages such as low energy cost for regeneration, tunable structure, elimination of corrosion problems, and additional advantages. However, one of the challenges facing this technology is to accomplish both high CO 2 capture capacity along with high CO2 diffusion rates concurrently. Current amine-based solid sorbents such as porous materials similar to SBA-15 have large pores diffusion entering molecules; however, the pores become clogged upon amine inclusion. To meet this challenge, our group's solution involves the creation of a new type of material which we are calling-amino-pillared nanosheet (APN) adsorbents which are generated from layered nanosheet precursors. These materials are being proposed because of their unique lamellar structure which exhibits ability to be modified by organic or inorganic pillars through consecutive swelling and pillaring steps to form large mesoporous interlayer spaces. After the expansion of the layer space through swelling and pillaring, the large pore space can be functionalized with amine groups. This selective functionalization is possible by the choice of amine group introduced. Our choice, large amine molecules, do not access the micropore within each layer; however, either physically or chemically immobilized onto the surface of the mesoporous interlayer space between each layer. The final goal of the research is to investigate the ability to prepare APN adsorbents from a model nanoporous layered materials including nanosheets precursor material MCM-22(P) and nanoporous layered silicate material AMH-3. MCM-22(P) contains 2-dimensional porous channels, 6 membered rings (MB) openings perpendicular to the layers and 10 MB channels in the plane of the layers. However, the transport limiting openings (6 MB) to the layers is smaller than CO2 gas molecules. In contrast, AMH-3 has

  17. Chapitre VI. Prudence du financement

    OpenAIRE

    Jambard, Pierre

    2015-01-01

    L’histoire financière de la société permet de comprendre bien des traits de son histoire. Comme toutes les entreprises du bâtiment et des travaux publics, la Société Auxiliaire s’est trouvée devant la nécessité de disposer d’importants capitaux circulants. Grâce à une gestion financière efficace, elle est, en général, parvenue à dégager de son activité les fonds nécessaires, bien que l’aisance de la trésorerie quotidienne n’ait été acquise qu’avec la reconversion. Les années 1927-1946 sont ce...

  18. Sulphite metabolism; Metabolisme du sulfite

    Energy Technology Data Exchange (ETDEWEB)

    Fromageot, P.; Chapeville, F. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1960-07-01

    Although the formation of sulphite by micro-organisms was observed as early as 1914 by Neuberg, it was only in 1932 that Nightingale showed it to be present inside cells, in the case of tomato leaf stalks, its formation being due to the reaction: SO{sub 3}H{sup -} + (S{sub 2}O{sub 3}){sub 2}{sup --} {yields} S{sub 2}O{sub 3}{sup --} + O{sub 3}S{sub 2}O{sub 3}{sup --}. The presence of the thiosulphate formed was shown by the high refractive index of its barium salt. Today we have a certain amount of data concerning the formation and use of sulphite in the living cell, even through the knowledge is still incomplete. In this article we will describe and discuss the facts which are known, with particular reference to the oxidation of sulphite. (author) [French] Si la formation de sulfite par des micro-organismes a ete observee des 1914 par Neuberg, sa presence a l'interieur des cellules a ete demontree pour la premiere fois par Nightingale en 1932, dans des tiges et des feuilles de tomates, par la reaction: SO{sub 3}H{sup -} + (S{sub 2}O{sub 3}){sub 2}{sup --} {yields} S{sub 2}O{sub 3}{sup --} + O{sub 3}S{sub 2}O{sub 3}{sup --}. Le thiosulfate forme etait mis en evidence par l'indice de refraction eleve de son sel de baryum. Aujourd'hui on possede un certain nombre de donnees, cependant encore tres incompletes, sur la formation du sulfite et son utilisation par la cellule vivante. Dans cet expose nous decrirons et discuterons quelques-unes de ces acquisitions, tout particulierement celles relatives a l'oxydation du sulfite. (auteur)

  19. Biogenic amines in Italian Pecorino cheese

    Directory of Open Access Journals (Sweden)

    Maria eSchirone

    2012-05-01

    Full Text Available The quality of distinctive artisanal cheeses is closely associated with the territory of production and its traditions. Pedoclimatic characteristics, genetic autochthonous variations and anthropic components create an environment so specific that it would be extremely difficult to reproduce elsewhere. Pecorino cheese is included in this sector of the market and is widely diffused in Italy (approximately 53.727t of production. Pecorino is a common name given to indicate Italian cheeses made exclusively from pure ewes' milk characterized by a high content of fat matter and it is mainly produced in the middle and south of Italy by traditional procedures from raw or thermized milk. The microbiota plays a major role in the development of the organoleptic characteristics of the cheese but it can also be responsible for the accumulation of undesirable substances, such as biogenic amines (BA. Several factors can contribute to the qualitative and quantitative profiles of BA’s in Pecorino cheese such as environmental hygienic conditions, pH, salt concentration, aw, fat content, pasteurization of milk, decarboxylase microorganisms, starter cultures, temperature and time of ripening, storage, part of the cheese (core, edge and the presence of cofactor. Generally, the total content of BA’s can range from about 100-2400 mg/kg, with a prevalence of toxicologically important BA’s, tyramine and histamine. The presence of BA in Pecorino cheeses is becoming increasingly important to consumers and cheese-maker alike, due to the potential threats of toxicity to humans and consequent trade implications.

  20. Secondary aerosol formation from atmospheric reactions of aliphatic amines

    Directory of Open Access Journals (Sweden)

    S. M. Murphy

    2007-01-01

    Full Text Available Although aliphatic amines have been detected in both urban and rural atmospheric aerosols, little is known about the chemistry leading to particle formation or the potential aerosol yields from reactions of gas-phase amines. We present here the first systematic study of aerosol formation from the atmospheric reactions of amines. Based on laboratory chamber experiments and theoretical calculations, we evaluate aerosol formation from reaction of OH, ozone, and nitric acid with trimethylamine, methylamine, triethylamine, diethylamine, ethylamine, and ethanolamine. Entropies of formation for alkylammonium nitrate salts are estimated by molecular dynamics calculations enabling us to estimate equilibrium constants for the reactions of amines with nitric acid. Though subject to significant uncertainty, the calculated dissociation equilibrium constant for diethylammonium nitrate is found to be sufficiently small to allow for its atmospheric formation, even in the presence of ammonia which competes for available nitric acid. Experimental chamber studies indicate that the dissociation equilibrium constant for triethylammonium nitrate is of the same order of magnitude as that for ammonium nitrate. All amines studied form aerosol when photooxidized in the presence of NOx with the majority of the aerosol mass present at the peak of aerosol growth consisting of aminium (R3NH+ nitrate salts, which repartition back to the gas phase as the parent amine is consumed. Only the two tertiary amines studied, trimethylamine and triethylamine, are found to form significant non-salt organic aerosol when oxidized by OH or ozone; calculated organic mass yields for the experiments conducted are similar for ozonolysis (15% and 5% respectively and photooxidation (23% and 8% respectively. The non-salt organic aerosol formed appears to be more stable than the nitrate salts and does not quickly repartition back to the gas phase.

  1. Mutagenic activity and heterocyclic amine content of the human diet

    Energy Technology Data Exchange (ETDEWEB)

    Knize, M.G.; Dolbeare, F.A.; Cunningham, P.L.; Felton, J.S.

    1993-01-15

    The mutagenic activity and the mass amount of heterocyclic amines responsible for the mutagenic activity have been measured in some cooked foods. Cooked meats are the predominant source of mutagenic activity in the diet with values ranging from 0 to 10,000 revertants per gram reported in the Ames/Salmonelia test with strain TA98. Several heterocyclic amines are present and have been quantified using solid-phase extraction followed by HPLC. Frying at higher temperatures and for longer times produces the greatest mutagenic response, and concomitantly, the largest amounts of heterocyclic amines. Most of the mutagenic activity in fried meat samples can be accounted for by MelQx, DiMelQx and IQ, although other heterocylic amines are present and PHIP mutagenic activity becomes significant at higher temperatures. Non-meat products such as baked breads can also form significant mutagenic activity, particularly when overcooked. Commercially prepared hamburgers made from meat substitutes such as tofu, wheat gluten or tempeh and fried at 210{degrees}C have up to 10% of the mutagenic activity of a fried beef patty cooked under the same conditions. When detected, amounts of heterocyclic amines in fried beef patties range from a total of 0.35 ng/g for commercial beef hamburgers to 142 ng/g for a beef patty cooked over a barbecue. Dietary intake is expected to have a large range, from less than one microgram per day to over 50 micrograms per day based on current knowledge of known heterocyclic amine chemicals and heterocyclic amine-containing foods.

  2. Base neurobiologique du rôle des récepteurs 5-HT1A dans le mode d'action des antidépresseurs sérotoninergiques

    OpenAIRE

    Gardier, A. M.; Jacquot, C.; Artigas, Francesc

    1995-01-01

    [FR]Les inhibiteurs de recapture sélectifs de la sérotonine (IRSS) bloquent l'activité du transporteur de la 5-hydroxy-tryptamine (5-HT) dépendante du sodium ; ils augmentent ainsi la concentration des mono-amines dans la fente synaptique. Le long délai (deux à quatre semaines) pour que les propriétés antidépressives se manifestent correspondrait au temps nécessaire à la désensibilisation fonctionnelle des autorécepteurs 5-HT1A des noyaux du raphé. La mesure des concentrations cérébrales extr...

  3. Inauguration du C/R Forum (Belgique

    Directory of Open Access Journals (Sweden)

    2009-04-01

    Full Text Available Le 16 février dernier, la Vlaamse beroepsvereniging Conservatie en Restauratie Forum (C/R Forum a été constituée à l'initiative de 18 personnes du milieu de la conservation/restauration et du secteur de la protection du patrimoine. Etant donné que la conservation et la gestion du patrimoine culturel mobilier et immobilier de même que la formation et la recherche relèvent des communautés et des régions, la nécessité est apparue depuis un certain temps de créer une organisation Flamande au sei...

  4. La fin du risque zéro : du homegrown jihadism au terrorisme du loup solitaire

    Directory of Open Access Journals (Sweden)

    Rémi Baudouï

    2015-09-01

    Full Text Available Le risque zéro représente un moment historique particulier dans la conceptualisation de la socio-politique du risque. Son application repose au début des années 1980 sur l’hypothèse de la possibilité de progresser dans le domaine de l’anticipation et de la gestion sécuritaire des crises potentielles au point de pouvoir prémunir les populations contre toute forme de menace. Il fut employé dans les domaines de la protection civile et de la sécurité militaire. Comme élément de gouvernance politique, il réfutait l’idée selon laquelle le risque existe en préalable comme catégorie d’acceptabilité collective de la menace.

  5. Aux frontières du politique et du religieux

    Directory of Open Access Journals (Sweden)

    Frédéric Sylvanise

    2006-05-01

    Full Text Available The Autobiography of Miss Jane Pittman met en scène de manière spectaculaire deux figures de leadership noir-américain au XIXe siècle (Ned et au XXe siècle (Jimmy. Ned, fils adoptif de Jane Pittman, se rebaptise Professor Douglass en hommage au leader abolitionniste Frederick Douglass. S’il est fondamentalement laïc (il est instituteur, le texte lui confère néanmoins une dimension religieuse, notamment dans le chapitre intitulé « The Sermon at the River » , qui renvoie à la fois à Moïse (le fleuve et à Jésus (le sermon sur la montagne. Abattu par un tueur à gages, Ned est un martyr dont l’histoire était déjà écrite. Le personnage de Jimmy, attendu par toute une communauté comme le messie (alors que lui aussi est un laïc et un homme qui perd la foi, s’inscrit dans la même histoire. Lettré, éloquent et impliqué dans le mouvement pour les droits civils, il est assassiné comme son prédécesseur. Dans les deux cas, le texte lie donc le politique et le biblique comme s’il ne faisait qu’un : être un leader politique, c’est être un preacher et un messie qui guide le peuple. Il est possible de lire l’évocation de ces deux hommes à la lumière d’une certaine actualité. Ned et Jimmy connaissent en effet un sort proche de celui de Malcolm X et de Martin Luther King, récemment assassinés au moment de l’écriture de The Autobiography of Miss Jane Pittman. C’est l’histoire de la répression du leadership noir, au travers du portrait de deux de ses avatars, qui peut donc se lire en creux ici.

  6. Polycyclic amines as chloroquine resistance modulating agents in Plasmodium falciparum.

    Science.gov (United States)

    Joubert, Jacques; Kapp, Erika; Taylor, Dale; Smith, Peter J; Malan, Sarel F

    2016-02-15

    Pentacycloundecylamines (PCUs) and adamantane amines, such as NGP1-01 (1) and amantadine, have shown significant channel blocking activities. They are postulated to act as chemosensitizers and circumvent the resistance of the plasmodia parasite against chloroquine (CQ) by inhibiting the p-glycoprotein efflux pump and enabling the accumulation of CQ inside the parasite digestive vacuole. Twelve polycyclic amines containing either a PCU or adamantane amine moiety conjugated to different aromatic functionalities through various tethered linkers were selected based on their channel blocking abilities and evaluated as potential chemosensitizers. Compounds 2, 4, 5 and 10 showed significant voltage-gated calcium channel (VGCC) blocking ability (IC50=0.27-35 μM) and were able to alter the CQ IC50 in differing degrees (45-81%) in the multidrug resistant Plasmodium falciparum Dd2 isolate. Among them, the PCU-dansyl amine compound (4) displayed the best potential to act as a chemosensitizer against the Dd2 strain at a 1 μM concentration (RMI=0.19) while displaying moderate antiplasmodial activity (Dd2 IC50=6.25 μM) and low in vitro cytotoxicity against a mammalian cell line (CHO, IC50=119 μM). Compounds 2 and 10 also showed some promising chemosensitizing abilities (RMI=0.36 and 0.35 respectively). A direct correlation was found between the VGCC blocking ability of these polycyclic amines and their capacity to act as CQ resistance modulating agents. PMID:26832222

  7. The Potential of the Yeast Debaryomyces hansenii H525 to Degrade Biogenic Amines in Food

    Directory of Open Access Journals (Sweden)

    Mathias Bäumlisberger

    2015-11-01

    Full Text Available Twenty-six yeasts from different genera were investigated for their ability to metabolize biogenic amines. About half of the yeast strains produced one or more different biogenic amines, but some strains of Debaryomyces hansenii and Yarrowia lipolytica were also able to degrade such compounds. The most effective strain D. hanseniii H525 metabolized a broad spectrum of biogenic amines by growing and resting cells. Degradation of biogenic amines by this yeast isolate could be attributed to a peroxisomal amine oxidase activity. Strain H525 may be useful as a starter culture to reduce biogenic amines in fermented food.

  8. PNA Peptide chimerae

    DEFF Research Database (Denmark)

    Koch, T.; Næsby, M.; Wittung, P.;

    1995-01-01

    Radioactive labelling of PNA has been performed try linking a peptide segment to the PNA which is substrate for protein kinase A. The enzymatic phosphorylation proceeds in almost quantitative yields....

  9. A Metal and Base-Free Chemoselective Primary Amination of Boronic Acids Using Cyanamidyl/Arylcyanamidyl Radical as Aminating Species: Synthesis and Mechanistic Studies by Density Functional Theory.

    Science.gov (United States)

    Chatterjee, Nachiketa; Arfeen, Minhajul; Bharatam, Prasad V; Goswami, Avijit

    2016-06-17

    An efficient, metal and base-free, chemoselective synthesis of aryl-, heteroaryl-, and alkyl primary amines from the corresponding boronic acids has been achieved at ambient temperature mediated by [bis(trifluoroacetoxy)iodo]benzene (PIFA) and N-bromosuccinimide (NBS) using cyanamidyl/arylcyanamidyl radicals as the aminating species. The primary amine compounds were initially obtained as their corresponding ammonium trifluoroacetate salts which, on treatment with aq NaOH, provide the free amines. Finally, the primary amines were isolated through column chromatography over silica-gel using hexane-EtOAc solvent system as the eluent. The reactions are sufficiently fast, completing within 1 h. Quantum chemical calculations in combination with experimental observations validate that the ipso amination of substituted boronic acids involves the formation of cyanamidyl/arylcyanamidyl radical, followed by regiospecific interaction of its nitrile-N center with boron atom of the boronic acids, leading to chemoselective primary amination. PMID:27182931

  10. Tumor penetrating peptides

    Directory of Open Access Journals (Sweden)

    Tambet eTeesalu

    2013-08-01

    Full Text Available Tumor-homing peptides can be used to deliver drugs into tumors. Phage library screening in live mice has recently identified homing peptides that specifically recognize the endothelium of tumor vessels, extravasate, and penetrate deep into the extravascular tumor tissue. The prototypic peptide of this class, iRGD (CRGDKGPDC, contains the integrin-binding RGD motif. RGD mediates tumor homing through binding to αv integrins, which are selectively expressed on various cells in tumors, including tumor endothelial cells. The tumor-penetrating properties of iRGD are mediated by a second sequence motif, R/KXXR/K. This C-end Rule (or CendR motif is active only when the second basic residue is exposed at the C-terminus of the peptide. Proteolytic processing of iRGD in tumors activates the cryptic CendR motif, which then binds to neuropilin-1 activating an endocytic bulk transport pathway through tumor tissue. Phage screening has also yielded tumor-penetrating peptides that function like iRGD in activating the CendR pathway, but bind to a different primary receptor. Moreover, novel tumor-homing peptides can be constructed from tumor-homing motifs, CendR elements and protease cleavage sites. Pathologies other than tumors can be targeted with tissue-penetrating peptides, and the primary receptor can also be a vascular zip code of a normal tissue. The CendR technology provides a solution to a major problem in tumor therapy, poor penetration of drugs into tumors. The tumor-penetrating peptides are capable of taking a payload deep into tumor tissue in mice, and they also penetrate into human tumors ex vivo. Targeting with these peptides specifically increases the accumulation in tumors of a variety of drugs and contrast agents, such as doxorubicin, antibodies and nanoparticle-based compounds. Remarkably the drug to be targeted does not have to be coupled to the peptide; the bulk transport system activated by the peptide sweeps along any compound that is

  11. Introduction to Peptide Synthesis

    OpenAIRE

    Stawikowski, Maciej; Fields, Gregg B.

    2002-01-01

    A number of synthetic peptides are significant commercial or pharmaceutical products, ranging from the dipeptide sugar-substitute aspartame to clinically used hormones, such as oxytocin, adrenocorticotropic hormone, and calcitonin. This unit provides an overview of the field of synthetic peptides and proteins. It discusses selecting the solid support and common coupling reagents. Additional information is provided regarding common side reactions and synthesizing modified residues.

  12. Solution Versus Gas-Phase Modification of Peptide Cations with NHS-Ester Reagents

    Science.gov (United States)

    Mentinova, Marija; Barefoot, Nathan Z.; McLuckey, Scott A.

    2012-02-01

    A comparison between solution and gas phase modification of primary amine sites in model peptide cations with N-hydroxysuccinimide (NHS) ester reagents is presented. In all peptides, the site of modification in solution was directed to the N-terminus by conducting reactions at pH = 5, whereas for the same peptides, a lysine residue was preferentially modified in the gas phase. The difference in pKa values of the N-terminus and ɛ-amino group of the lysine allows for a degree of control over sites of protonation of the peptides in aqueous solution. With removal of the dielectric and multiple charging of the peptide ions in the gas phase, the accommodation of excess charge can affect the preferred sites of reaction. Interaction of the lone pair of the primary nitrogen with a proton reduces its nucleophilicity and, as a result, its reactivity towards NHS-esters. While no evidence for reaction of the N-terminus with sulfo-NHS-acetate was noted in the model peptide cations, a charge inversion experiment using bis[sulfosuccinimidyl] suberate, a cross-linking reagent with two sulfo-NHS-ester functionalities, showed modification of the N-terminus. Hence, an unprotonated N-terminus can serve as a nucleophile to displace NHS, which suggests that its lack of reactivity with the peptide cations is likely due to the participation of the N-terminus in solvating excess charge.

  13. Abiotic formation of valine peptides under conditions of high temperature and high pressure.

    Science.gov (United States)

    Furukawa, Yoshihiro; Otake, Tsubasa; Ishiguro, Takato; Nakazawa, Hiromoto; Kakegawa, Takeshi

    2012-12-01

    We investigated the oligomerization of solid valine and the stabilities of valine and valine peptides under conditions of high temperature (150-200 °C) and high pressure (50-150 MPa). Experiments were performed under non-aqueous condition in order to promote dehydration reaction. After prolonged exposure of monomeric valine to elevated temperatures and pressures, the products were analyzed by liquid chromatography mass spectrometry comparing their retention times and masses. We identified linear peptides that ranged in size from dimer to hexamer, as well as a cyclic dimer. Previous studies that attempted abiotic oligomerization of valine in the absence of a catalyst have never reported valine peptides larger than a dimer. Increased reaction temperature increased the dissociative decomposition of valine and valine peptides to products such as glycine, β-alanine, ammonia, and amines by processes such as deamination, decarboxylation, and cracking. The amount of residual valine and peptide yields was greater at higher pressures at a given temperature, pressure, and reaction time. This suggests that dissociative decomposition of valine and valine peptides is reduced by pressure. Our findings are relevant to the investigation of diagenetic processes in prebiotic marine sediments where similar pressures occur under water-poor conditions. These findings also suggest that amino acids, such as valine, could have been polymerized to peptides in deep prebiotic marine sediments within a few hundred million years.

  14. Abiotic Formation of Valine Peptides Under Conditions of High Temperature and High Pressure

    Science.gov (United States)

    Furukawa, Yoshihiro; Otake, Tsubasa; Ishiguro, Takato; Nakazawa, Hiromoto; Kakegawa, Takeshi

    2012-12-01

    We investigated the oligomerization of solid valine and the stabilities of valine and valine peptides under conditions of high temperature (150-200 °C) and high pressure (50-150 MPa). Experiments were performed under non-aqueous condition in order to promote dehydration reaction. After prolonged exposure of monomeric valine to elevated temperatures and pressures, the products were analyzed by liquid chromatography mass spectrometry comparing their retention times and masses. We identified linear peptides that ranged in size from dimer to hexamer, as well as a cyclic dimer. Previous studies that attempted abiotic oligomerization of valine in the absence of a catalyst have never reported valine peptides larger than a dimer. Increased reaction temperature increased the dissociative decomposition of valine and valine peptides to products such as glycine, β-alanine, ammonia, and amines by processes such as deamination, decarboxylation, and cracking. The amount of residual valine and peptide yields was greater at higher pressures at a given temperature, pressure, and reaction time. This suggests that dissociative decomposition of valine and valine peptides is reduced by pressure. Our findings are relevant to the investigation of diagenetic processes in prebiotic marine sediments where similar pressures occur under water-poor conditions. These findings also suggest that amino acids, such as valine, could have been polymerized to peptides in deep prebiotic marine sediments within a few hundred million years.

  15. Identification of SNAIL1 Peptide-Based Irreversible Lysine-Specific Demethylase 1-Selective Inactivators.

    Science.gov (United States)

    Itoh, Yukihiro; Aihara, Keisuke; Mellini, Paolo; Tojo, Toshifumi; Ota, Yosuke; Tsumoto, Hiroki; Solomon, Viswas Raja; Zhan, Peng; Suzuki, Miki; Ogasawara, Daisuke; Shigenaga, Akira; Inokuma, Tsubasa; Nakagawa, Hidehiko; Miyata, Naoki; Mizukami, Tamio; Otaka, Akira; Suzuki, Takayoshi

    2016-02-25

    Inhibition of lysine-specific demethylase 1 (LSD1), a flavin-dependent histone demethylase, has recently emerged as a new strategy for treating cancer and other diseases. LSD1 interacts physically with SNAIL1, a member of the SNAIL/SCRATCH family of transcription factors. This study describes the discovery of SNAIL1 peptide-based inactivators of LSD1. We designed and prepared SNAIL1 peptides bearing a propargyl amine, hydrazine, or phenylcyclopropane moiety. Among them, peptide 3, bearing hydrazine, displayed the most potent LSD1-inhibitory activity in enzyme assays. Kinetic study and mass spectrometric analysis indicated that peptide 3 is a mechanism-based LSD1 inhibitor. Furthermore, peptides 37 and 38, which consist of cell-membrane-permeable oligoarginine conjugated with peptide 3, induced a dose-dependent increase of dimethylated Lys4 of histone H3 in HeLa cells, suggesting that they are likely to exhibit LSD1-inhibitory activity intracellularly. In addition, peptide 37 decreased the viability of HeLa cells. We believe this new approach for targeting LSD1 provides a basis for development of potent selective inhibitors and biological probes for LSD1. PMID:26700437

  16. Biokinetics and dosimetry of depleted uranium (DU) in rats implanted with DU fragments.

    Energy Technology Data Exchange (ETDEWEB)

    Guilmette, Ray A.; Hahn, Fletcher F.; Durbin, P. W.

    2004-01-01

    A number of U. S. veterans of the Persian Gulf War were wounded with depleted uranium (DU) metal fragments as a result of 'friendly fire' incidents, in which Abrams tanks and Bradley fighting vehicles were struck by DU anti-armor munitions. Some of the crew members who survived were left with multiple small fragments of DU in their muscles and soft tissues. The number, size and location of the fragments made them inoperable in general, and therefore subject to long-term retention. Because there was inadequate data to predict the potential carcinogenicity of DU fragments in soft tissues, Hahn et al. (2003) conducted a lifespan cancer study in rats. As part of that study, a number of rats were maintained to study the biokinetics and dosimetry of DU implanted intramuscularly in male Wistar rats. Typically, four metal fragments, either as cylindrical pellets or square wafers were implanted into the biceps femoris muscles of the rats. Urine samples were collected periodically during their lifespans, and DU was analyzed in kidneys and eviscerated carcass (minus the implant sites) at death. The daily DU urinary excretion rate increased steeply during the first 30 d after implantation peaking at about 90 d at 3-10 x 10{sup -3}%/d. During the first 150 d, the average excretion rate was 2.4 x 10{sup -3}%/d, decreasing thereafter to about 1 x 10{sup -3}%/d. Serial radiographs were made of the wound sites to monitor gross morphologic changes in the DU implant and the surrounding tissue. As early as 1 w after implantation, radiographs showed the presence of surface corrosion and small, dense bodies near the original implant, presumably DU. This corrosion from the surface of the implant continued with time, but did not result in an increasing amount of DU reaching the blood and urine after the first 3 mo. During this 3-mo period, connective tissue capsules formed around the implants, and are hypothesized to have reduced the access of DU to tissue fluids by limiting the

  17. Synthesis and inverse emulsion polymerization of aminated acrylamidodextran.

    Science.gov (United States)

    Daubresse, C; Grandfils, C; Jérôme, R; Teyssié, P; Goethals, P; Schacht, E

    1993-12-01

    A chemically modified form of dextran was prepared, having a polymerizable moiety (acrylamide) and a reactive functional group (primary amine). Dextran was activated with 4-nitrophenyl-chloroformate (24 mol per polysaccharide, 9.8 mol per 100 glucose residues); 9.8% glucose residues were converted to aliphatic carbonates and 5.2% were converted to cyclic carbonates. The activated dextran was coupled with trityldiaminoethane (8 mol per 100 glucose residues), reactivated with 4-nitrophenylchloroformate, then coupled with acryloamidodiaminohexane (6.8 mol per 100 glucose residues). The trityl group was removed by hydrolysis with trifluoroacetic acid to yield the required aminated acryloamidodextran. The modified dextran was shown to be polymerizable by inverse emulsion polymerization. Submicron particles were successfully prepared, containing functional amine groups suitable for preparing drug conjugates or for modifying the surface properties of this biomaterial. PMID:7512648

  18. Holographic property of photopolymers with different amine photoinitiators

    International Nuclear Information System (INIS)

    Holographic parameters and photobleaching kinetics of the photopolymers with five different amine photoinitiators are studied. The maximum values of diffraction efficiency, photobleaching coefficient and quantum yield follow the sequence: Triethanolamine (TEA)> Diethanolamine (DEA)> Ethanolamine (EA)> Triethylamine (TETN)> Diethylamine (DETN). The holographic capabilities of photopolymer performances are determined by the number of functional groups in the amine molecular structure. There is an optimum proportion of the photoinitiator, the photosensitizer and the monomer in the test of holographic parameters with different amine concentrations. The maximum diffraction efficiency is 59.26%, sensitivity is 1.72 × 10−3 cm2/mJ, and the maximum refractive modulation index is 4.64×10−4. (classical areas of phenomenology)

  19. Skin absorption of six performance amines used in metalworking fluids.

    Science.gov (United States)

    Roux, Lauriane N; Brooks, James D; Yeatts, James L; Baynes, Ronald E

    2015-05-01

    Every year, 10 million workers are exposed to metalworking fluids (MWFs) that may be toxic. There are four types of MWFs: neat oils and three water-based MWFs (soluble oil, semisynthetic and synthetic), which are diluted with water and whose composition varies according to the mineral oils ratio. MWFs also contain various additives. To determine the absorption of six amines used as corrosion inhibitors and biocides in MWFs, porcine skin flow-through diffusion cell experiments were conducted with hydrophilic ethanolamines (mono-, di- and triethanolamine, MEA, DEA and TEA respectively) and a mixture of lipophilic amines (dibutylethanolamine, dicyclohexylamine and diphenylamine). The six amines were dosed in four vehicles (water and three generic water-based MWF formulations) and analyzed using a scintillation counter or gas chromatography/mass spectrometry. These 24 h studies showed that dermal absorption significantly (P metalworkers. PMID:25186650

  20. Quantification of Tryptic Peptides in Quadrupole Ion Trap Using High-Mass Signals Derived from Isotope-Coded N-Acetyl Dipeptide Tags

    Science.gov (United States)

    Seo, Jongcheol; Yoon, Hye-Joo; Shin, Seung Koo

    2011-09-01

    Isotope-labeled N-acetyl dipeptides (Ac-Xxx-Ala) are coupled to the primary amines of tryptic peptides and then analyzed by tandem mass spectrometry. Amide bond cleavage between Xxx and Ala provides both low- and high-mass isotope-coded signals for quantification of peptides. Especially, facile cleavage at the modified lysine side chain yields very strong high-mass quantitation signals in a noise-free region. Tagging tryptic peptides with isobaric N-acetyl dipeptides is a viable strategy for accurate quantification of proteins, which can be used with most quadrupole ion trap mass spectrometers carrying the 1/3 mass cut-off problem.

  1. Bases chimiosensorielles du comportement alimentaire chez les poissons

    Directory of Open Access Journals (Sweden)

    SAGLIO Ph.

    1981-07-01

    Full Text Available Le comportement alimentaire, indispensable à la survie de l'individu et donc de l'espèce, occupe à ce titre une position de première importance dans la hiérarchie des comportements fondamentaux qui tous en dépendent très étroitement. Chez les poissons, cette prééminence se trouve illustrée par l'extrême diversité des supports sensoriels impliqués et des expressions comportementales qui leur sont liées. A la suite d'un certain nombre de mises en évidence neurophysiologiques et éthologiques de l'importance du sens chimique (olfaction, gustation dans le comportement alimentaire des poissons, de très importants secteurs d'études électrophysiologiques et d'analyses physico-chimiques visant à en déterminer la nature exacte (en termes de substances actives se sont développés ces vingt dernières années. De tous ces travaux dont les plus avancés sont présentés ici, il ressort que les acides aminés de série L plus ou moins associés à d'autres composés de poids moléculaires < 1000 constituent des composés chimiques jouant un rôle déterminant dans le comportement alimentaire de nombreuses espèces de poissons carnivores.

  2. Skin absorption of six performance amines used in metalworking fluids.

    Science.gov (United States)

    Roux, Lauriane N; Brooks, James D; Yeatts, James L; Baynes, Ronald E

    2015-05-01

    Every year, 10 million workers are exposed to metalworking fluids (MWFs) that may be toxic. There are four types of MWFs: neat oils and three water-based MWFs (soluble oil, semisynthetic and synthetic), which are diluted with water and whose composition varies according to the mineral oils ratio. MWFs also contain various additives. To determine the absorption of six amines used as corrosion inhibitors and biocides in MWFs, porcine skin flow-through diffusion cell experiments were conducted with hydrophilic ethanolamines (mono-, di- and triethanolamine, MEA, DEA and TEA respectively) and a mixture of lipophilic amines (dibutylethanolamine, dicyclohexylamine and diphenylamine). The six amines were dosed in four vehicles (water and three generic water-based MWF formulations) and analyzed using a scintillation counter or gas chromatography/mass spectrometry. These 24 h studies showed that dermal absorption significantly (P < 0.05) increased from water for the six amines (e.g. 1.15 ± 0.29% dose; DEA in water) compared to other formulations (e.g. 0.13 ± 0.01% dose; DEA in semisynthetic MWF) and absorption was greatest for dibutylethanolamine in all the formulations. The soluble oil formulation tended to increase the dermal absorption of the hydrophilic amines. The permeability coefficient was significantly higher (P < 0.05) with TEA relative to the other hydrophilic amines (e.g. 4.22 × 10(-4) ± 0.53 × 10(-4) cm h(-1) [TEA in synthetic MWF] vs. 1.23 × 10(-4) ± 0.10 × 10(-4) cm h(-1) [MEA in synthetic MWF]), except for MEA in soluble oil formulation. Future research will confirm these findings in an in vivo pig model along with dermatotoxicity studies. These results should help MWF industries choose safer additives for their formulations to protect the health of metalworkers.

  3. Le respect du droit du travail : entre politique de contrôle et politique pénale

    OpenAIRE

    Auvergnon, Philippe; Laviolette, Sandrine

    2004-01-01

    Le respect du droit du travail – ou sa moindre ineffectivité - tient en partie à l'action de l'inspection du travail. Dans quelle mesure s'inscrit-elle dans une politique de contrôle ? L'action pénale, elle-même inscrite ou non dans une politique pénale, peut aussi contribuer au respect du droit du travail en sanctionnant le relevé d'infractions. Le présent rapport tente de revisiter les pratiques et les relations entretenues entre Inspection du travail et Parquet, à partir d'une part de l'an...

  4. Amine-intercalated α-zirconium phosphates as lubricant additives

    International Nuclear Information System (INIS)

    In this study, three types of amines intercalated α-zirconium phosphate nanosheets with different interspaces were synthesized and examined as lubricant additives to a mineral oil. Results from tribological experiments illustrated that these additives improved lubricating performance. Results of rheological experiments showed that the viscosity of the mineral oil was effectively reduced with the addition of α-zirconium phosphate nanosheets. The two-dimensional structure, with larger interspaces, resulting from amine intercalation, exhibited improved effectiveness in reducing viscosity. This study demonstrates that the nanosheet structure of α-zirconium phosphates is effective in friction reduction. The manufacture of lubricants with tailored viscosity is possible by using different intercalators

  5. Highly Efficient N-Monomethylation of Primary Aryl Amines

    Institute of Scientific and Technical Information of China (English)

    PENG, Yiyuan; LIU, Hanliang; TANG, Min; CAI, Lisheng; PIKE, Victor

    2009-01-01

    A highly efficient method for specific synthesis of N-monomethylarylamines is presented. Anilines were treated with acetic anhydride and triethylamine in dry CH2Cl2 to give the corresponding acetamides. The subsequent N-monomethylation of acetyl aryl amines with methyl iodide and Nail in THF introduced methyl group. Acid hy- drolysis of the N-methyl acetanilides in ethylene glycol generated the corresponding N-methyl-N-aryi amines in high yields. This method was also used to synthesize (E)-2-bromo-5-(4-methylaminostyryl)pyridine that may be useful as an amyloid imaging agent for Alzheimer's disease.

  6. Economic Considerations for Selecting an Amine Donor in Biocatalytic Transamination

    DEFF Research Database (Denmark)

    Tufvesson, Pär; Nordblad, Mathias; Krühne, Ulrich;

    2015-01-01

    The industrial implementation of biocatalysis for production of pharma and fine chemicals has grown substantially over recent years. An upcoming application is that of chiral synthesis of optically pure amines, a technology known for many years but that is now seeing a renewed and wider interest ...... process, in particular the choice of amine donor. This paper discusses these constraints and demonstrates, through simple thermodynamic and economic models, the process targets that need to be set and achieved for a process dependent on allowed process costs and quality targets....

  7. Studies in biogenic amine metabolism by mass spectrometry

    International Nuclear Information System (INIS)

    Two areas of mass spectral study related to biogenic amine metabolism are presented: The use of electron capture negative ion chemical ionization mass spectrometry for the quantitation of melatonin and other indole amines, and general synthetic procedures useful for the synthesis of deuterated diazomethane and deuteromethylated catechols. The factors determining instrumental sensitivity in negative ion chemical ionization are discussed, and the enhancement of the primary ion beam using magnetic fields is described. Quantitation of human plasma melatonin at the parts per trillion or pg/ml level has been demonstrated and is routinely performed as a selected ion monitoring assay. (Auth.)

  8. Influence of beer storage for the selected biogenic amines content

    OpenAIRE

    Brýdlová, Nikola

    2012-01-01

    This thesis does not only changes in the content of biogenic amines during storage of bottled beer, but beer in general. The production of beer in the world is gradually increasing. In 2010, produced a 811,4 million hectoliters of beer. Czech Republic in 2010 produced 17,1 million hectoliters in 2011 and ceased production decline. Average consumption in the Czech Republic was in 2010, 144 liters/person/year. Another chapter is devoted to biogenic amines. They are nitrogen compounds, in which ...

  9. Determination of Biogenic Amines in Different Shrimp Species for Export

    International Nuclear Information System (INIS)

    This study is part of the project on the ''Quality Assurance of Different Shrimp Species for Export''. Local shrimp samples were collected from Ministry of Livestock and Fisheries and various private enterprises. Contents of biogenic amines were determined by using benzoyl chloride derivatization method with HPLC (reverse phase high performance liquid chromatography). Based on the biogenic amines, quality index of shrimps were correlated with freshness index so that the grade of shrimp samples can be classified as excellent, good, and acceptable. All sizes of shrimps such as extra large, large, medium were found to excceptable respectively

  10. Mesoporous amine-bridged polysilsesquioxane for CO2 capture

    KAUST Repository

    Qi, Genggeng

    2011-01-01

    A novel class of amine-supported sorbents based on amine-bridged mesoporous polysilsesquioxane was developed via a simple one-pot sol-gel process. The new sorbent allows the incorporation of a large amount of active groups without sacrificing surface area or pore volume available for CO2 capture, leading to a CO2 capture capacity of 3.2 mmol g−1 under simulated flue gas conditions. The sorbent is readily regenerated at 100°C and exhibits good stability over repetitive adsorption-desorption cycling.

  11. Biogenic amines in smear and mould-ripened cheeses

    Directory of Open Access Journals (Sweden)

    Pavel Pleva

    2014-02-01

    Full Text Available The aim of the study was the monitoring of six biogenic amines (histamine, tyramine, phenylethylamine, tryptamine, putrescine, and cadaverine and two polyamines (spermidine and spermine in 30 samples of dairy products purchased in the Czech Republic, namely in 15 samples of mould-ripened cheeses and in 15 samples of smear-ripened cheeses. A further goal was the microbiological analysis of the individual samples of cheeses (total count of microorganisms, number of enterobacteria, enterococci, lactic acid bacteria, yeasts and moulds. The monitored biogenic amines were analyzed by a high performance liquid chromatography equipped with a UV/VIS DAD detector. The amount of enterobacteria in fresh cheese exceeded 105 CFU.g‑1. In smear-ripened cheese flavourless (Romadur type, the amount was >103 CFU.g-1 and 104-105 CFU.g-1 in smear-ripened cheese with flavour. Biogenic amines were observed in two groups of blue cheeses (white veined cheese and blue veined cheese and smear-ripened cheeses. In both groups, there is a possibility of the presence of biogenic amines because the number of microorganisms and concentration of free amino acids increase during ripening. In ten samples of soft smear-ripening acid cheese and in smear-ripened cheese, the total content of biogenic amines were 22-1000 mg.kg-1 and in 5 samples of these cheeses, it was in range 1000-6000 mg.kg-1. The total amount of biogenic amines in the blue cheeses were in range 40-600 mg.kg-1. The presense of the tyramine was observed in the all analysed cheeses. The tyramine producing strains generated more than 900 mg.kg-1 of this biogenic amine. The production of tryptamine in the analysed cheeses was not proved by this study. The results of this study show that biogenic amines and polyamines are common in cheese. However, in some cases, they can pose a significant health danger for consumers. Any legislative control authority does not monitor them, as they are secondary metabolites even

  12. Enzymatic network for production of ether amines from alcohols

    DEFF Research Database (Denmark)

    Palacio, Cyntia M.; Crismaru, Ciprian G.; Bartsch, Sebastian;

    2016-01-01

    We constructed an enzymatic network composed of three different enzymes for the synthesis of valuable ether amines. The enzymatic reactions are interconnected to catalyze the oxidation and subsequent transamination of the substrate and to provide cofactor recycling. This allows production...... of the desired ether amines from the corresponding ether alcohols with inorganic ammonium as the only additional substrate. To examine conversion, individual and overall reaction equilibria were established. Using these data, it was found that the experimentally observed conversions of up to 60% observed...

  13. Investigation of impregnation methods in amine modification of mesoporous solids

    OpenAIRE

    Daniels, Emma; Madden, David; Curtin, Teresa

    2013-01-01

    non-peer-reviewed Carbon dioxide is one of a number of gases known as green-house gases which is thought to contribute to global climate change. CO2 released from fossil-fuel fired electric power generation plants is believed to be one of the main contributors to these emissions. A potential way to deal with these CO2 emissions is carbon dioxide capture and storage. This research investigates the application of amine-modified mesoporous silica as CO2 adsorbents. Various amine modification...

  14. Aux marges du monde arabe

    Directory of Open Access Journals (Sweden)

    Hélène Thiollet

    2004-09-01

    Full Text Available L’étude des migrations contemporaines des Érythréens vers le Yémen au tournant des années quatre-vingt-dix permet d’observer les transformations des dynamiques régionales à l’œuvre dans le monde arabe. Les migrations sont un phénomène sensible aux différents aspects (politiques, économiques, culturels, géographiques de l’intégration régionale. Celle si est envisagée dans cet article à travers une approche doublement marginale : -la marginalité géographique de l’Érythrée arabo-africaine et du Yémen, économi­quement isolé dans la péninsule Arabique, -l’étude des migrations formelles et informelles, élément souvent marginal dans l’étude des institutions et des échanges qui constituent un système régional intégré. Cette étude est fondée sur une série d’entretiens menés au Yémen entre février et avril 2002 auprès des administrations yéménites et de la population érythréenne.

  15. DU COTÉ DE CHEZ SOI

    Directory of Open Access Journals (Sweden)

    Marc Smeets

    2013-12-01

    Full Text Available Le voyage en Hollande, au XIXe siècle, s'inscrit dans une longue tradition où résonnent les notions de liberté, tolérance et commerce, mais il acquiert aussi une spécificité étant donné l’importance que prend la figure du « chez soi » : les Pays-Bas, terre d'agrément où le voyageur français se sent à l'aise et où il aimerait, si possible, vivre. Proust, de ce point de vue-là, ne fait rien de neuf quand il rêve dans la Recherche d'une « vie domestique » en terre batave. Les Pays-Bas, pour le voyageur français au XIXe, c'est le home sweet home.

  16. Etude du comportement biogéochimique du carbone dans le lac Kivu au nord-ouest du Rwanda

    OpenAIRE

    Rwabuhungu Rwatangabo, Digne

    2008-01-01

    RésuméLe Rift Est-Africain comprend plusieurs grands lacs, dont le lac Kivu situé entre 1°34’et 2°30’ de latitude Sud et compris entre 28°50’ et 29°23’ de longitude Est. Ce lac,localisé au nord-ouest du Rwanda à la frontière avec la République Démocratique duCongo, présente une spécificité unique au monde: ses eaux profondes contiennentune gigantesque quantité de gaz dissous (3/4 de dioxyde de carbone (CO2), 1/4 degaz méthane (CH4)).Les études antérieures indiquent que les eaux du lac Kivu pr...

  17. Chapitre XI. Normes familiales et usages du droit

    OpenAIRE

    Martin, Claude

    2015-01-01

    En analysant 24 configurations familiales recomposées, nous avons pu apprécier comment se différencient les attitudes à l’égard du droit en fonction du capital culturel, du projet conjugal et du degré ou du type de communication dans le réseau familial élargi. En effet, comme nous l’avons évoqué, il est possible de distinguer différents usages du droit : du point de vue du droit civil, soit les acteurs évitent d’y recourir et préfèrent des situations de fait qui correspondent à des accords né...

  18. Influence of the immobilized yeast cells technology on the presence of biogenic amines in wine

    OpenAIRE

    Miličević, Borislav; Šubarić, Drago; Babić, Jurislav; Ačkar, Đurđica; Jozinović, Antun; Petošić, Emil; Matijević, Anita

    2014-01-01

    Biogenic amines are basic nitrogenous low molecular weight compounds with biological activity. Biogenic amines are important because they contain a health risk for sensitive humans. Biogenic amines in the wine can be formed from their precursors by various microorganisms present in the wine, at any stage of production. The aim of the present work was to study the changes of the content of biogenic amines in wines made from grape variety Frankovka and Pinot noir (Vitis vinifera L.) from Kutjev...

  19. The Potential of the Yeast Debaryomyces hansenii H525 to Degrade Biogenic Amines in Food

    OpenAIRE

    Mathias Bäumlisberger; Urs Moellecken; Helmut König; Harald Claus

    2015-01-01

    Twenty-six yeasts from different genera were investigated for their ability to metabolize biogenic amines. About half of the yeast strains produced one or more different biogenic amines, but some strains of Debaryomyces hansenii and Yarrowia lipolytica were also able to degrade such compounds. The most effective strain D. hanseniii H525 metabolized a broad spectrum of biogenic amines by growing and resting cells. Degradation of biogenic amines by this yeast isolate could be attributed to a pe...

  20. Établissements recevant du public

    CERN Document Server

    2014-01-01

    Synthèse pour les installations électriques, des règles de sécurité contre les risques d’incendie et de panique dans les établissements recevant du public (ERP), selon l’arrêté du 25 juin 1980 modifié. L'ouvrage traite à la fois des dispositions communes aux établissements du 1er groupe (1ère à 4ème catégorie), du 2ème groupe (5ème catégorie) et des règles particulières applicables aux différents types d'établissements (structures d'accueil pour personnes âgées ou handicapées, hôtels, magasins de vente, restaurants et débit de boisson, établissements de soins, administration, châpiteaux, etc...) Sont ainsi notamment traités, dans le cadre des dispositions communes aux établissements du 1er groupe, pour les installations électriques normales : les installations des appareils, tableaux et canalisations, les locaux électriques et installation de machines, l'éclairage normal des locaux accessibles au public, le chauffage et la ventilation, l'installation d'eau chaude sanita...

  1. THE UPTAKE OF CATECHOL AMINES AT HIGH PERFUSION CONCENTRATIONS IN THE RAT ISOLATED HEART: A NOVEL CATECHOL AMINE UPTAKE PROCESS

    OpenAIRE

    Iversen, LL

    1997-01-01

    In previous studies of the uptake of catechol amines in the rat isolated heart it was shown that adrenaline and noradrenaline were accumulated by a common mechanism (Iversen, 1963, 1965). In these experiments the uptake saturated at an external amine concentration of approximately 0.2 μg/ml. noradrenaline or 0.5 μg/ml. adrenaline. However, in subsequent experiments in which hearts were perfused with (±)-adrenaline at a concentration of 5.0 μg/ml. an unexpectedly large uptake of adrenaline was...

  2. Antimicrobial Peptides from Plants

    Science.gov (United States)

    Tam, James P.; Wang, Shujing; Wong, Ka H.; Tan, Wei Liang

    2015-01-01

    Plant antimicrobial peptides (AMPs) have evolved differently from AMPs from other life forms. They are generally rich in cysteine residues which form multiple disulfides. In turn, the disulfides cross-braced plant AMPs as cystine-rich peptides to confer them with extraordinary high chemical, thermal and proteolytic stability. The cystine-rich or commonly known as cysteine-rich peptides (CRPs) of plant AMPs are classified into families based on their sequence similarity, cysteine motifs that determine their distinctive disulfide bond patterns and tertiary structure fold. Cystine-rich plant AMP families include thionins, defensins, hevein-like peptides, knottin-type peptides (linear and cyclic), lipid transfer proteins, α-hairpinin and snakins family. In addition, there are AMPs which are rich in other amino acids. The ability of plant AMPs to organize into specific families with conserved structural folds that enable sequence variation of non-Cys residues encased in the same scaffold within a particular family to play multiple functions. Furthermore, the ability of plant AMPs to tolerate hypervariable sequences using a conserved scaffold provides diversity to recognize different targets by varying the sequence of the non-cysteine residues. These properties bode well for developing plant AMPs as potential therapeutics and for protection of crops through transgenic methods. This review provides an overview of the major families of plant AMPs, including their structures, functions, and putative mechanisms. PMID:26580629

  3. Antimicrobial Peptides from Plants

    Directory of Open Access Journals (Sweden)

    James P. Tam

    2015-11-01

    Full Text Available Plant antimicrobial peptides (AMPs have evolved differently from AMPs from other life forms. They are generally rich in cysteine residues which form multiple disulfides. In turn, the disulfides cross-braced plant AMPs as cystine-rich peptides to confer them with extraordinary high chemical, thermal and proteolytic stability. The cystine-rich or commonly known as cysteine-rich peptides (CRPs of plant AMPs are classified into families based on their sequence similarity, cysteine motifs that determine their distinctive disulfide bond patterns and tertiary structure fold. Cystine-rich plant AMP families include thionins, defensins, hevein-like peptides, knottin-type peptides (linear and cyclic, lipid transfer proteins, α-hairpinin and snakins family. In addition, there are AMPs which are rich in other amino acids. The ability of plant AMPs to organize into specific families with conserved structural folds that enable sequence variation of non-Cys residues encased in the same scaffold within a particular family to play multiple functions. Furthermore, the ability of plant AMPs to tolerate hypervariable sequences using a conserved scaffold provides diversity to recognize different targets by varying the sequence of the non-cysteine residues. These properties bode well for developing plant AMPs as potential therapeutics and for protection of crops through transgenic methods. This review provides an overview of the major families of plant AMPs, including their structures, functions, and putative mechanisms.

  4. Electron transfer in peptides.

    Science.gov (United States)

    Shah, Afzal; Adhikari, Bimalendu; Martic, Sanela; Munir, Azeema; Shahzad, Suniya; Ahmad, Khurshid; Kraatz, Heinz-Bernhard

    2015-02-21

    In this review, we discuss the factors that influence electron transfer in peptides. We summarize experimental results from solution and surface studies and highlight the ongoing debate on the mechanistic aspects of this fundamental reaction. Here, we provide a balanced approach that remains unbiased and does not favor one mechanistic view over another. Support for a putative hopping mechanism in which an electron transfers in a stepwise manner is contrasted with experimental results that support electron tunneling or even some form of ballistic transfer or a pathway transfer for an electron between donor and acceptor sites. In some cases, experimental evidence suggests that a change in the electron transfer mechanism occurs as a result of donor-acceptor separation. However, this common understanding of the switch between tunneling and hopping as a function of chain length is not sufficient for explaining electron transfer in peptides. Apart from chain length, several other factors such as the extent of the secondary structure, backbone conformation, dipole orientation, the presence of special amino acids, hydrogen bonding, and the dynamic properties of a peptide also influence the rate and mode of electron transfer in peptides. Electron transfer plays a key role in physical, chemical and biological systems, so its control is a fundamental task in bioelectrochemical systems, the design of peptide based sensors and molecular junctions. Therefore, this topic is at the heart of a number of biological and technological processes and thus remains of vital interest.

  5. Electromembrane extraction of peptides.

    Science.gov (United States)

    Balchen, Marte; Reubsaet, Léon; Pedersen-Bjergaard, Stig

    2008-06-20

    Rapid extraction of eight different peptides using electromembrane extraction (EME) was demonstrated for the first time. During an extraction time of 5 min, the model peptides migrated from a 500 microL aqueous acidic sample solution, through a thin supported liquid membrane (SLM) of an organic liquid sustained in the pores in the wall of a porous hollow fiber, and into a 25 microL aqueous acidic acceptor solution present inside the lumen of the hollow fiber. The driving force of the extraction was a 50 V potential sustained across the SLM, with the positive electrode in the sample and the negative electrode in the acceptor solution. The nature and the composition of the SLM were highly important for the EME process, and a mixture of 1-octanol and 15% di(2-ethylhexyl) phosphate was found to work properly. Using 1mM HCl as background electrolyte in the sample and 100 mM HCl in the acceptor solution, and agitation at 1050 rpm, enrichment up to 11 times was achieved. Recoveries were found to be dependent on the structure of the peptide, indicating that the polarity and the number of ionized groups were important parameters affecting the extraction efficiency. The experimental findings suggested that electromembrane extraction of peptides is possible and may be a valuable tool for future extraction of peptides. PMID:18479691

  6. Blue Chitin columns for the extraction of heterocyclic amines from urine samples

    DEFF Research Database (Denmark)

    Bang, J.; Frandsen, Henrik Lauritz; Skog, K.

    2004-01-01

    . Blue Chitin columns were used for the extraction and purification of heterocyclic amines from urine samples spiked with 14 different heterocyclic amines. The samples were analysed using LC-MS. The results show that Blue Chitin columns provide a straightforward and rapid means of extracting heterocyclic...... amines from urine samples, and that Blue Chitin column are also useful in the purification of urinary metabolites....

  7. Recent achievements in developing the biocatalytic toolbox for chiral amine synthesis.

    Science.gov (United States)

    Kohls, Hannes; Steffen-Munsberg, Fabian; Höhne, Matthias

    2014-04-01

    Novel enzyme activities and chemoenzymatic reaction concepts have considerably expanded the biocatalytic toolbox for chiral amine synthesis. Creating new activities or extending the scope of existing enzymes by protein engineering is a common trend in biocatalysis and in chiral amine synthesis specifically. For instance, an amine dehydrogenase that allows for the direct asymmetric amination of ketones with ammonia was created by mutagenesis of an l-amino acid dehydrogenase. Another trend in chiral amine chemistry is the development of strategies allowing for the synthesis of secondary amines. For example the smart choice of substrates for amine transaminases provided access to secondary amines by chemoenzymatic reactions. Furthermore novel biocatalysts for the synthesis of secondary amines such as imine reductases and Pictet-Spenglerases have been identified and applied. Recent examples showed that the biocatalytic amine synthesis is emerging from simple model reactions towards industrial scale preparation of pharmaceutical relevant substances, for instance, as shown in the synthesis of a Janus kinase 2 inhibitor using an amine transaminase. A comparison of important process parameters such as turnover number and space-time yield demonstrates that biocatalytic strategies for asymmetric reductive amination are maturing and can already compete with established chemical methods. PMID:24721252

  8. Aerosol and cloud chemistry of amines from CCS - reactivity experiments and numerical modeling

    Science.gov (United States)

    Weller, Christian; Tilgner, Andreas; Herrmann, Hartmut

    2013-04-01

    Capturing CO2 from the exhaust of power plants using amine scrubbing is a common technology. Therefore, amines can be released during the carbon capture process. To investigate the tropospheric chemical fate of amines from CO2 capturing processes and their oxidation products, the impact of aqueous aerosol particles and cloud droplets on the amine chemistry has been considered. Aqueous phase reactivity experiments of NO3 radicals and ozone with relevant amines and their corresponding nitrosamines were performed. Furthermore, nitrosamine formation and nitrosamine photolysis was investigated during laboratory experiments. These experiments implicated that aqueous phase photolysis can be an effective sink for nitrosamines and that ozone is unreactive towards amines and nitrosamines. Multiphase phase oxidation schemes of amines, nitrosamines and amides were developed, coupled to the existing multiphase chemistry mechanism CAPRAM and built into the Lagrangian parcel model SPACCIM using published and newly measured data. As a result, both deliquescent particles and cloud droplets are important compartments for the multiphase processing of amines and their products. Amines can be readily oxidised by OH radicals in the gas and cloud phase during daytime summer conditions. However, amine oxidation is restricted during winter conditions with low photochemical activity leading to long lifetimes of amines. The importance of the gas and aqueous phase depends strongly on the partitioning of the different amines. Furthermore, the simulations revealed that the aqueous formation of nitrosamines in aerosol particles and could droplets is not a relevant process under tropospheric conditions.

  9. A ratiometric fluorescent probe for detection of biogenic primary amines with nanomolar sensitivity.

    Science.gov (United States)

    Mallick, Suman; Chandra, Falguni; Koner, Apurba L

    2016-02-01

    An ultrasensitive ratiometric fluorescent sensor made of an N,N-dimethylaminonaphthalene anhydride moiety for detection of aliphatic primary amines is reported. Biogenic amines at nanomolar concentration is detected with the additional ability to discriminate between primary, secondary and tertiary amines by using both UV-Visible and fluorescence spectroscopy. PMID:26734688

  10. Synthesis of sulfonamides via copper-catalyzed oxidative C-N bond cleavage of tertiary amines.

    Science.gov (United States)

    Ji, Jing; Liu, Zhengyi; Liu, Ping; Sun, Peipei

    2016-08-01

    A copper-catalyzed coupling reaction of sulfonyl chlorides with tertiary amines via the oxidative C-N bond cleavage of tertiary amines was developed. Sulfonamides were synthesized using this strategy in moderate to good yields. The reaction was applicable to various tertiary amines, as well as sulfonyl chlorides. PMID:27356858

  11. Heterogeneous uptake of amines by citric acid and humic acid.

    Science.gov (United States)

    Liu, Yongchun; Ma, Qingxin; He, Hong

    2012-10-16

    Heterogeneous uptake of methylamine (MA), dimethylamine (DMA), and trimethylamine (TMA) onto citric acid and humic acid was investigated using a Knudsen cell reactor coupled to a quadrupole mass spectrometer at 298 K. Acid-base reactions between amines and carboxylic acids were confirmed. The observed uptake coefficients of MA, DMA, and TMA on citric acid at 298 K were measured to be 7.31 ± 1.13 × 10(-3), 6.65 ± 0.49 × 10(-3), and 5.82 ± 0.68 × 10(-3), respectively, and showed independence of sample mass. The observed uptake coefficients of MA, DMA, and TMA on humic acid at 298 K increased linearly with sample mass, and the true uptake coefficients of MA, DMA, and TMA were measured to be 1.26 ± 0.07 × 10(-5), 7.33 ± 0.40 × 10(-6), and 4.75 ± 0.15 × 10(-6), respectively. Citric acid, having stronger acidity, showed a higher reactivity than humic acid for a given amine; while the steric effect of amines was found to govern the reactivity between amines and citric acid or humic acid.

  12. The Reactions of 3, 7-Dinitrodibenzobromolium Salt with Some Amines

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The reactions of 3,7-dinitrodibenzobromolium salt with some amines were studied. A reaction mechanism based on the structure of the major product 6 and the minor product 7 was proposed. The reaction was considered to proceed via a substituted benzyene intermediate.

  13. The repertoire of trace amine G-protein-coupled receptors

    DEFF Research Database (Denmark)

    Gloriam, David E.; Bjarnadóttir, Thóra K; Yan, Yi-Lin;

    2005-01-01

    eukaryotic species for receptors similar to the mammalian trace amine (TA) receptor subfamily. We identified 18 new receptors in rodents that are orthologous to the previously known TA-receptors. Remarkably, we found 57 receptors (and 40 pseudogenes) of this type in the zebrafish (Danio rerio), while fugu...

  14. Iron catalysed direct alkylation of amines with alcohols

    NARCIS (Netherlands)

    Yan, Tao; Feringa, Ben L; Barta, Katalin

    2014-01-01

    The selective conversion of carbon-oxygen bonds into carbon-nitrogen bonds to form amines is one of the most important chemical transformations for the production of bulk and fine chemicals and pharma intermediates. An attractive atom-economic way of carrying out such C-N bond formations is the dire

  15. Advances in Biochemical Screening for Phaeochromocytoma using Biogenic Amines

    OpenAIRE

    Whiting, Malcolm J; Doogue, Matthew P

    2009-01-01

    Biochemical testing for phaeochromocytoma is performed in diagnostic laboratories using a variety of tests with plasma, serum or 24-hour urine collections. These tests include catecholamines and their methylated metabolites - the metanephrines, either individually or in combination with their sulfated metabolites. High-performance liquid chromatography (HPLC) continues to be the dominant analytical method for biogenic amine quantitation. Chromatographic techniques are changing, with improveme...

  16. Cloud condensation nuclei activity of aliphatic amine secondary aerosol

    Science.gov (United States)

    Aliphatic amines can form secondary aerosol via oxidation with atmospheric radicals (e.g. hydroxyl radical and nitrate radical). The resulting particle composition can contain both secondary organic aerosol (SOA) and inorganic salts. The fraction of organic to inorganic materials in the particulate ...

  17. Inhibition of chickpea seedling copper amine oxidases by tetraethylenepentamine

    Directory of Open Access Journals (Sweden)

    Sona Talaei

    2012-01-01

    Full Text Available Copper amine oxidases are important enzymes, which contribute to the regulation of mono- and polyamine levels. Each monomer contains one Cu(II ion and 2,4,5-trihydroxyphenylalanine (TPQ as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to molecular oxygen which is reduced to hydrogen peroxide. Inhibitors are important tools in the study of catalytic properties of copper amine oxidases and they also have a wide application in physiological research. In this study, purification of the chickpea seedling amine oxidase, was done via salting out by ammonium sulfate and dialysis, followed by DEAE-cellulose column chromatography. By using the Lineweaver - Burk plot, the Km and Vm of the enzyme were found to be 3.3 mM and 0.95 mmol/min/mg, respectively. In this study, the interaction of chickpea diamino oxidase with tetraethylene- pentamine was studied. Analysis of kinetic data indicated that tetraethylenepentamine (with Ki=0.1 mM inhibits the enzyme by linear mixed inhibitory effect.

  18. Synthesis and Properties of An Aminated hypocrellin B

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A new aminated-perylenequinone derivative 2 was synthesized by the reaction of 1 ( hypocrellin B ) with 6-aminohexanoic-acid. The structure of 2 was identified by UV-Vis, MS, IR and 1H-NMR. The amphiphility, spectrum and photochemical properties of 2 were reported.

  19. AROMATIC AMINES IN AND NEAR THE BUFFALO RIVER

    Science.gov (United States)

    Three sediment samples taken from the Buffalo River and two soil samples taken near its bank have been analyzed for 2-propanol-extractable, basic organic compounds by using GC/MS. Eleven aromatic amines related to the commercial production of malachite green and crystal violet we...

  20. Effects of amine fluoride on biofilm growth and salivary pellicles

    NARCIS (Netherlands)

    Van der Mei, HC; Engels, E; de Vries, Jacob; Busscher, HJ

    2008-01-01

    The amine fluoride (AmF) N'-octadecyltrimethylendiamine-N, N,N'-tris(2-ethanol)-dihydrofluoride is a cationic antimicrobial which can have beneficial effects on plaque formation. Here, we determine changes in pellicle and bacterial cell surface properties of the strains Actinomyces naeslundii HM1, S

  1. Therapeutic HIV Peptide Vaccine

    DEFF Research Database (Denmark)

    Fomsgaard, Anders

    2015-01-01

    infection directed to subdominant conserved HIV-1 epitopes restricted to frequent HLA supertypes. The rationale for selecting HIV peptides and adjuvants are provided. Peptide subunit vaccines are regarded as safe due to the simplicity, quality, purity, and low toxicity. The caveat is reduced immunogenicity......Therapeutic vaccines aim to control chronic HIV infection and eliminate the need for lifelong antiretroviral therapy (ART). Therapeutic HIV vaccine is being pursued as part of a functional cure for HIV/AIDS. We have outlined a basic protocol for inducing new T cell immunity during chronic HIV-1...... and hence adjuvants are included to enhance and direct the immune response. Although the vaccine has been tested in ART naïve individuals, we recommend future testing of the vaccine during (early started) ART that improves immune function and to select individuals likely to benefit. Peptides representing...

  2. Synthetic antibiofilm peptides.

    Science.gov (United States)

    de la Fuente-Núñez, César; Cardoso, Marlon Henrique; de Souza Cândido, Elizabete; Franco, Octavio Luiz; Hancock, Robert E W

    2016-05-01

    Bacteria predominantly exist as multicellular aggregates known as biofilms that are associated with at least two thirds of all infections and exhibit increased adaptive resistance to conventional antibiotic therapies. Therefore, biofilms are major contributors to the global health problem of antibiotic resistance, and novel approaches to counter them are urgently needed. Small molecules of the innate immune system called host defense peptides (HDPs) have emerged as promising templates for the design of potent, broad-spectrum antibiofilm agents. Here, we review recent developments in the new field of synthetic antibiofilm peptides, including mechanistic insights, synergistic interactions with available antibiotics, and their potential as novel antimicrobials against persistent infections caused by biofilms. This article is part of a Special Issue entitled: Antimicrobial peptides edited by Karl Lohner and Kai Hilpert. PMID:26724202

  3. Recherche sur les Juifs du Maroc

    OpenAIRE

    Michel Abitbol

    2008-01-01

    Le Centre de recherche sur les Juifs du Maroc (CRJM) a été créé, en mai 1994, à Paris à l’initiative de Monsieur Robert Assaraf, après de longs mois de concertations, de discussions et d’études préalables. Il s’agit d’une institution internationale, à vocation universitaire et scientifique qui s’est fixé pour but essentiel de développer, par tous les moyens, la connaissance et l’étude de l’histoire des Juifs du Maroc. Une histoire trois fois millénaire qui remonte à la destruction du premier ...

  4. Pouvoir du parasol et pouvoir nu

    OpenAIRE

    Dakhlia, Jocelyne

    2010-01-01

    Delacroix a immortalisé la posture altière du sultan marocain à cheval à travers plusieurs œuvres : dans ces représentations, le parasol incarne l’emblème par excellence de la dynastie alaouite et de la royauté marocaine dans son ensemble. Tout en retraçant rapidement l’histoire du parasol comme objet régalien dans le monde musulman, on s’interrogera sur une certaine discordance entre l’image très imposante de la royauté que perçoivent les observateurs européens du xixe siècle et une traditio...

  5. Théories du nombre

    OpenAIRE

    Reynié, Dominique

    1988-01-01

    La naissance de l'État moderne est allée de pair avec l'apparition de son grand vis-à-vis : le nombre. Depuis l'émergence de la politique moderne, on trouve une pluralité de discours qui tentent de théoriser la place des masses dans la société civile et politique. Aux théories du XIXe siècle dominées par une crainte plus ou moins avouée du nombre, succèdent celles du XXe siècle qui le présentent au contraire comme le grand sujet de la domination. La mise en valeur de ce renversement de pro...

  6. Les Barthes, Labastide-du-Temple, Lizac

    OpenAIRE

    Hautefeuille, Florent

    2013-01-01

    Date de l'opération : 1991 (PR) Inventeur(s) : Hautefeuille Florent La prospection diachronique entreprise sur les trois communes avait pour but de déterminer l’importance de l’occupation humaine mais aussi d’envisager la restitution du couvert végétal au travers d’une esquisse d’archéologie du paysage. La zone couverte, plus de 3000 ha, s’insère dans un contexte géographique qui se situe autour de deux méandres du Tarn, entre sa confluence avec la Garonne et celle, plus en amont, avec l’Avey...

  7. L'épreuve du feu

    OpenAIRE

    Pécout, Roland

    2007-01-01

    Entre Causses et Cévennes au Nord, et plaine littorale au sud, les collines calcaires du Bas-Languedoc sont couvertes d'une formation végétale particulière, qui va de la lande au taillis, et du taillis à la forêt. Ces immenses étendues sèches, presque inhabitées, peuvent apparaître au premier regard comme le domaine d'une nature intacte... Ce sont pourtant des terres façonnées par l'usage agro-pastoral depuis fort longtemps, et qui ne sont redevenues « sauvages » que tout récemment. Age du cu...

  8. Biomimetic peptide nanosensors.

    Science.gov (United States)

    Cui, Yue; Kim, Sang N; Naik, Rajesh R; McAlpine, Michael C

    2012-05-15

    The development of a miniaturized sensing platform tailored for sensitive and selective detection of a variety of biochemical analytes could offer transformative fundamental and technological opportunities. Due to their high surface-to-volume ratios, nanoscale materials are extremely sensitive sensors. Likewise, peptides represent robust substrates for selective recognition due to the potential for broad chemical diversity within their relatively compact size. Here we explore the possibilities of linking peptides to nanosensors for the selective detection of biochemical targets. Such systems raise a number of interesting fundamental challenges: What are the peptide sequences, and how can rational design be used to derive selective binders? What nanomaterials should be used, and what are some strategies for assembling hybrid nanosensors? What role does molecular modeling play in elucidating response mechanisms? What is the resulting performance of these sensors, in terms of sensitivity, selectivity, and response time? What are some potential applications? This Account will highlight our early attempts to address these research challenges. Specifically, we use natural peptide sequences or sequences identified from phage display as capture elements. The sensors are based on a variety of nanomaterials including nanowires, graphene, and carbon nanotubes. We couple peptides to the nanomaterial surfaces via traditional surface functionalization methods or self-assembly. Molecular modeling provides detailed insights into the hybrid nanostructure, as well as the sensor detection mechanisms. The peptide nanosensors can distinguish chemically camouflaged mixtures of vapors and detect chemical warfare agents with sensitivities as low as parts-per-billion levels. Finally, we anticipate future uses of this technology in biomedicine: for example, devices based on these sensors could detect disease from the molecular components in human breath. Overall, these results provide a

  9. Aux sources de l'histoire du pays soninké : le cas du Kingi (Mali)

    OpenAIRE

    Chastanet, Monique

    1990-01-01

    Le livre de M. DIAWARA, La graine de la parole... dont il est question dans cet article, traite des différents types de traditions orales historiques de l'Etat du Kingi, du 15ème au milieu du 19ème siècle. Il met en rapport leurs fonctions socio-politiques avec les transformations de cette société et analyse leurs modes de transmission, plus ou moins formalisés. Cette étude concerne aussi bien les traditions familiales que celles des spécialistes et souligne, notamment, le rôle des femmes et ...

  10. Dicyclopropylmethyl peptide backbone protectant.

    Science.gov (United States)

    Carpino, Louis A; Nasr, Khaled; Abdel-Maksoud, Adel Ali; El-Faham, Ayman; Ionescu, Dumitru; Henklein, Peter; Wenschuh, Holger; Beyermann, Michael; Krause, Eberhard; Bienert, Michael

    2009-08-20

    The N-dicyclopropylmethyl (Dcpm) residue, introduced into amino acids via reaction of dicyclopropylmethanimine hydrochloride with an amino acid ester followed by sodium cyanoborohydride or triacetoxyborohydride reduction, can be used as an amide bond protectant for peptide synthesis. Examples which demonstrate the amelioration of aggregation effects include syntheses of the alanine decapeptide and the prion peptide (106-126). Avoidance of cyclization to the aminosuccinimide followed substitution of Fmoc-(Dcpm)Gly-OH for Fmoc-Gly-OH in the assembly of sequences containing the sensitive Asp-Gly unit.

  11. Invertebrate FMRFamide related peptides.

    Science.gov (United States)

    Krajniak, Kevin G

    2013-06-01

    In 1977 the neuropeptide FMRFamide was isolated from the clam, Macrocallista nimbosa. Since then several hundred FMRFamide-related peptides (FaRPs) have been isolated from invertebrate animals. Precursors to the FaRPs likely arose in the cnidarians. With the transition to a bilateral body plan FaRPs became a fixture in the invertebrate phyla. They have come to play a critical role as neurotransmitters, neuromodulators, and neurohormones. FaRPs regulate a variety of body functions including, feeding, digestion, circulation, reproduction, movement. The evolution of the molecular form and function of these omnipresent peptides will be considered.

  12. 肽类肠内营养制剂的益处%Benefits of peptide-based enteral nutrition

    Institute of Scientific and Technical Information of China (English)

    PamelaR.Poberts

    2001-01-01

    Theories of protein digestion have classically asserted thatproteins are completely hydrolyzed to free amino acids within the intestine before absorption occurs.Further,it has been taught that only free amino acids enter the circulation.However,current evidence indicates that hydrolyzed protein fragments(i.e.peptides)also cross the small intestine and reach peripheral tissue via the systemic circulation,Nitrogen sources for enteral nutrition are free amino acids,peptides,or intact proteins.Current experimental evidence indicates that diets which possess the capability of producing luminal peptides are superior to diets lacking this capacity.The parent protein used for enteral nutrition generates specific peptides which may dictate a variety of metabolic responses. Many small peptides derived from the diet possess bioligic activity and may also play a role in regulating physiologic processes.Dietary peptides can have specific actions either locally,on the gastrointestinal tract,or at more distant sites.These peptides may alter cellular metabolism and may act as vasoregulators,growth factors,releasing hormones,,or neurotransmitters.The concept of dietary bioactive peptides offers an explanation for varying effects of diet on physiologic responses.These concepts have spurred research efforts into the possibility of enteral administration of biogenic amines.

  13. Présentation du dossier

    OpenAIRE

    2008-01-01

    L’objet du présent dossier est d’interroger des pratiques d'entretiens qui visent l'élucidation et la conscientisation par l'apprenant de son propre fonctionnement mental en situation d'apprentissage. Cette réflexion vise à confronter trois approches, certes différentes, mais qui poursuivent des finalités voisines et pourraient s'avérer complémentaires : celle du dialogue pédagogique, pratiqué en gestion mentale, celle de l’entretien de type critique, inspiré des travaux de Jean Piaget, et en...

  14. Geographical and diurnal features of amine-enhanced boundary layer nucleation

    Science.gov (United States)

    Bergman, T.; Laaksonen, A.; Korhonen, H.; Malila, J.; Dunne, E. M.; Mielonen, T.; Lehtinen, K. E. J.; Kühn, T.; Arola, A.; Kokkola, H.

    2015-09-01

    Amines have recently been found to be an important ingredient in the nucleation and initial growth of atmospheric aerosols; however, global estimates of the spatial and temporal extent of amine-enhanced nucleation are currently missing. We utilize two recently published laboratory data sets of amine-sulfuric acid nucleation to evaluate the accuracy of previously published nucleation parameterizations and to produce a new amine-enhanced new particle formation (NPF) parameterization that better reproduces the laboratory observations at atmospherically relevant sulfuric acid concentrations. We implement and compare the amine-enhanced NPF parameterizations and a kinetic nucleation parameterization within the global aerosol-climate model ECHAM-HAMMOZ and find that the spatial features of amine-enhanced and kinetic NPF are clearly different. Amine-enhanced NPF is limited to areas near the source regions of amine due to its short gas phase residence time of 6.9 h, whereas kinetic nucleation (which depends only on sulfuric acid concentration) produces particles more uniformly across the globe due to long-range transport of SO2. The notably stronger land-sea contrast in amine-enhanced nucleation simulations is in line with relatively rare atmospheric observations of NPF over open oceans. However, when the uptake of gas phase amine molecules to aerosol particles is limited according to previously published estimates (0.2% of collisions leading to uptake), the amine-enhanced NPF parameterization predicts in some regions unrealistically high NPF rates (˜1000 cm-3 s-1) compared to typical observations. Our results indicate that amine-enhanced nucleation may be an important particle formation mechanism near amine source regions but also highlights the need for more tightly defined constraints on the spatial and temporal distribution of amine emissions, gas-to-particle partitioning mechanisms of amines, and condensation and coagulation sinks in global models.

  15. INFLUENCE OF MOLECULAR STRUCTURES OF SECONDARY AMINE TERMINATED POLY(ESTER-AMINE)S ON THE CURING PERFORMANCE WITH EPOXY RESIN

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Five secondary amine terminated poly(ester-amine)s (defined as PEA) with controlled molecular structures were synthesized through reacting excessive piperazine with phthalicdiglycol diacrylate (PDDA) and 1,1,1-trimethylolpropane triacrylate (TMPTA) at a constant secondary amine/acrylate group ratio of 1.5/1 and at different PDDA/TMPTA molar ratios.Both IR and 1H-NMR spectra indicated that all acrylate groups were consumed in the reaction, based on which the structural parameters were calculated from the 1H-NMR spectra. With decreasing PDDA/TMPTA ratio, the content of secondary amine, degree of branching, molecular weight, Tg and Td increased accordingly. These polymers were further used as both crosslinkers and flexibilizers for a linear epoxy resin E51 to form cured films under ambient condition, The gel content,relative hardness and Tg of the resulting films increased as PEA molecules changed from linear to highly branching structures. Due to the flexibility of PEA molecules, all the films possessed excellent mechanical performance.

  16. Le Flaubert de Charles Du Bos

    Directory of Open Access Journals (Sweden)

    Jacques Neefs

    2009-01-01

    Full Text Available Charles Du Bos a porté une attention constante à l’œuvre de Flaubert (à l’exclusion de Bouvard et Pécuchet qui semble ne pas exister pour lui, à Madame Bovary et à L’Éducation sentimentale en particulier. La mise en relation de son étude : « Sur le milieu intérieur chez Flaubert », écrite en 1921, avec des textes du Journal de 1923 et de 1937, les rapprochements avec Gogol, Thomas Hardy, Tolstoï, Baudelaire, Henry James qui traversent les écrits de Du Bos, permettent de suivre ce que celui-ci décrit comme « l’expérience spirituelle » d’une matérialité comprise dans la conquête de la triple exigence du Beau, du Vivant et du Vrai. Du Bos décèle la force de l’œuvre de Flaubert dans la « disproportion » du style, et dans la puissance d’absorption qui fait la densité de cette prose, et qui désigne un extraordinaire travail de conversion. L’obscure expérience spirituelle ainsi poursuivie est celle d’un absolu de l’art, expérience paradoxale d’un « mystique qui ne croit à rien » (comme se désignait Flaubert lui-même, que le critique lie à une interrogation sur sa propre conversion.Charles Du Bos devoted an unflagging attention to Flaubert’s work (except for Bouvard et Pécuchet, which, apparently, according to him did not exist, to Madame Bovary and in particular L’Éducation sentimentale. The connection between his essay “Sur le milieu intérieur chez Flaubert”, written in 1921, and extracts from his Journal, from 1923 to 1937, the comparisons with Gogol, Thomas Hardy, Tolstoy, Baudelaire, and Henry James that run through the writings of Du Bos, allow us to follow what he terms “the spiritual experience” of a materiality encompassed in the conquest of the triple demand of the Beautiful, the Living, the Truth. Du Bos detects the power of Flaubert’s work in the “disproportion” of his style, and the power of absorption that forms the density of his prose, showing an

  17. Natriuretic peptides and cerebral hemodynamics

    DEFF Research Database (Denmark)

    Guo, Song; Barringer, Filippa; Zois, Nora Elisabeth;

    2014-01-01

    Natriuretic peptides have emerged as important diagnostic and prognostic tools for cardiovascular disease. Plasma measurement of the bioactive peptides as well as precursor-derived fragments is a sensitive tool in assessing heart failure. In heart failure, the peptides are used as treatment...

  18. Towards biomimetic scaffolds: anhydrous scaffold fabrication from biodegradable amine-reactive diblock copolymers.

    Science.gov (United States)

    Hacker, Michael; Tessmar, Jörg; Neubauer, Markus; Blaimer, Andrea; Blunk, Torsten; Göpferich, Achim; Schulz, Michaela B

    2003-11-01

    The development of biomimetic materials and their processing into three-dimensional cell carrying scaffolds is one promising tissue engineering strategy to improve cell adhesion, growth and differentiation on polymeric constructs developing mature and viable tissue. This study was concerned with the fabrication of scaffolds made from amine-reactive diblock copolymers, N-succinimidyl tartrate monoamine poly(ethylene glycol)-block-poly(D,L-lactic acid), which are able to suppress unspecific protein adsorption and to covalently bind proteins or peptides. An appropriate technique for their processing had to be both anhydrous, to avoid hydrolysis of the active ester, and suitable for the generation of interconnected porous structures. Attempts to fabricate scaffolds utilizing hard paraffin microparticles as hexane-extractable porogens failed. Consequently, a technique was developed involving lipid microparticles, which served as biocompatible porogens on which the scaffold forming polymer was precipitated in the porogen extraction media (n-hexane). Porogen melting during the extraction and polymer precipitation step led to an interconnected network of pores. Suitable lipid mixtures and their melting points, extraction conditions (temperature and time) and a low-toxic polymer solvent system were determined for their use in processing diblock copolymers of different molecular weights (22 and 42 kDa) into highly porous off-the-shelf cell carriers ready for easy surface modification towards biomimetic scaffolds. Insulin was employed to demonstrate the principal of instant protein coupling to a prefabricated scaffold. PMID:12922156

  19. A DNA biosensor based on the electrocatalytic oxidation of amine by a threading intercalator

    Energy Technology Data Exchange (ETDEWEB)

    Gao Zhiqiang [Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669 (Singapore)], E-mail: zqgao@ibn.a-star.edu.sg; Tansil, Natalia [Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669 (Singapore)

    2009-03-16

    An electrochemical biosensor for the detection of DNA based a peptide nucleic acid (PNA) capture probe (CP) modified indium tin oxide electrode (ITO) is described in this report. After hybridization, a threading intercalator, N,N'-bis[(3-propyl)-imidazole]-1,4,5,8-naphthalene diimide (PIND) imidazole complexed with Ru(bpy){sub 2}Cl (PIND-Ru, bpy = 2,2'-bipyridine), was introduced to the biosensor. PIND-Ru selectively intercalated to double-stranded DNA (ds-DNA) and became immobilized on the biosensor surface. Voltammetric tests showed highly stable and reversible electrochemical oxidation/reduction processes and the peak currents can directly be utilized for DNA quantification. When the tests were conducted in an amine-containing medium, Tris-HCl buffer for example, a remarkable improvement in the voltammetric response and noticeable enhancements of voltammetric and amperometric sensitivities were observed due to the electrocatalytic activity of the [Ru(bpy){sub 2}Cl] redox moieties. Electrocatalytic current was observed when as little as 3.0 attomoles of DNA was present in the sample solution.

  20. L'évolution du peuplement sudarabique du Ier au VIe siècle

    OpenAIRE

    Schiettecatte, Jérémie

    2008-01-01

    Cette contribution s'arrête sur la question suivante : un déclin du peuplement s'observe-t-il en Arabie du Sud à la veille de l'islam ? Et si tel est le cas, comment l'expliquer ? Le traitement cartographique des données nous permet dans un premier temps d'observer l'évolution du réseau urbain sudarabique. Celui-ci semble s'étioler. Dans un second temps, la documentation est considérée d'un point de vue critique, afin de cerner dans quelle mesure une diminution de la densité du réseau urbain ...

  1. Figurations du crime, figurations du lieu commun de la littérature moderne

    OpenAIRE

    Daros, Philippe

    2015-01-01

    Peut-être de façon générale est-on en droit de suggérer une double polarité, absolument dialectique d’ailleurs, sur les figurations du roman du crime. Selon la première, historiquement constitutive de ce sous-genre romanesque, le crime, comme événement fictionnel fondateur du muthos, est le support même de la représentation comme configuration d’un ordre du monde interprétable par la raison et, je le suppose, Hegel aurait été grand consommateur de cette littérature où le sujet non maître de s...

  2. Johannes Angermuller, Analyse du discours poststructuraliste. Les voix du sujet chez Lacan, Althusser, Foucault, Derrida, Sollers

    OpenAIRE

    Guilhaumou, Jacques

    2014-01-01

    Si l’approche foucaldienne du discours apparaît quelque peu hybride au regard des développements récents en pragmatique du discours, il n’en reste pas moins que Johannes Angermuller y voit, au titre de l’accent mis dans L’archéologie du savoir sur l’instance de l’événement énonciatif, un modèle interprétatif propice à une compréhension globale du champ de l’analyse de discours, donc de sa construction par étapes au cours des années soixante-dix / quatre-vingt. En effet, le point de vue foucal...

  3. Descriptors for antimicrobial peptides

    DEFF Research Database (Denmark)

    Jenssen, Håvard

    2011-01-01

    Introduction: A frightening increase in the number of isolated multidrug resistant bacterial strains linked to the decline in novel antimicrobial drugs entering the market is a great cause for concern. Cationic antimicrobial peptides (AMPs) have lately been introduced as a potential new class of ...

  4. L’ombre du Condor

    Directory of Open Access Journals (Sweden)

    Franck Gaudichaud

    2003-09-01

    Full Text Available Si l’on observe aujourd’hui, avec un regard d’historien, le Cône Sud à la fin des années soixante soixante-dix et que par, la suite, on tourne une page de quelques années, pour fixer la même partie du monde, le constat est sans appel : le sous-continent latino-américain est passé, en général, d’une phase de forte mobilisation et politisation sociale, montée en puissance de partis et organisations révolutionnaires, surgissements de gouvernements populistes de gauche ou progressistes – appelant à une rupture avec l’impérialisme –, à un reflux généralisé du mouvement ouvrier, une ère de violence politique étatisée, la destruction massive de tous les espaces d’expression et de participation démocratiques, l’écrasement physique et idéologique sans relâche des militants et mouvements révolutionnaires, la mise en place de modèles économiques capitalistes dirigistes puis/ou néolibéraux. Que s’est-il passé ? Quel fil conducteur a été rompu et par quels moyens l’Amérique Latine a été conduite à ce reflux des luttes sociales ? Pour répondre à cette question, nous nous contenterons dans cet article de focaliser notre objectif sur un aspect spécifique et essentiel de ce retournement de tendance que vit alors l���Amérique Latine : celui de la mise en place d’un terrorisme d’Etat contre-révolutionnaire transnational, appuyé par l’impérialisme américain, nommé « l’Opération Condor ». Dans cet article, il s’agit de montrer le fonctionnement d’un terrorisme hégémonique dont l’impact sur les sociétés latino-américaines est considérable en termes de destruction du mouvement social, des activités culturelles et de l’ensemble de la vie sociale et politique qui restent marquées, encore aujourd’hui à des degrés divers, par la violence, la peur et l’atomisation structurelle.Si miramos hoy en día, desde una perspectiva histórica, el Cono Sur hacia finales de

  5. Preparation of shell cross-linked nano-objects from hybrid-peptide block copolymers.

    Science.gov (United States)

    Rodríguez-Hernández, Juan; Babin, Jérôme; Zappone, Bruno; Lecommandoux, Sébastien

    2005-01-01

    Supramolecular structures formed by self-assembly of diblock copolymers in solution are stable over restricted environmental conditions: concentration, temperature, pH, or ion strength among others. To enlarge their domain of application, it appears necessary to develop stabilization strategies. We report here different strategies to stabilize the shell of micelles formed by self-assembly of amphiphilic polydiene-b-polypeptide diblock copolymers. For this purpose, covalent bonds can be formed between either amine or carboxylic acid groups distributed along the soluble peptide block and a cross-linking agent that contains respectively aldehyde or amine functions. Shell stabilization affords systems with unique properties that combine three main advantages: shape persistence, control of the porosity, and stimuli-responsive behavior. The covalent capture of such macromolecular objects has been studied by light scattering, AFM, and conductimetry measurements.

  6. OGM : vers une définition commune du contenu du problème ?

    Directory of Open Access Journals (Sweden)

    Icart Jean-Claude

    2001-07-01

    Full Text Available Les divergences croissantes depuis des années entre experts, responsables économiques, et acteurs sociaux et « citoyens » au sujet des OGM, ont pu être analysées à juste titre, comme exprimant la difficulté de dégager une « définition commune du contenu du problème »1.

  7. Biochemical functionalization of peptide nanotubes with phage displayed peptides

    Science.gov (United States)

    Swaminathan, Swathi; Cui, Yue

    2016-09-01

    The development of a general approach for the biochemical functionalization of peptide nanotubes (PNTs) could open up existing opportunities in both fundamental studies as well as a variety of applications. PNTs are spontaneously assembled organic nanostructures made from peptides. Phage display has emerged as a powerful approach for identifying selective peptide binding motifs. Here, we demonstrate for the first time the biochemical functionalization of PNTs via peptides identified from a phage display peptide library. The phage-displayed peptides are shown to recognize PNTs. These advances further allow for the development of bifunctional peptides for the capture of bacteria and the self-assembly of silver particles onto PNTs. We anticipate that these results could provide significant opportunities for using PNTs in both fundamental studies and practical applications, including sensors and biosensors nanoelectronics, energy storage devices, drug delivery, and tissue engineering.

  8. Biochemical functionalization of peptide nanotubes with phage displayed peptides.

    Science.gov (United States)

    Swaminathan, Swathi; Cui, Yue

    2016-09-01

    The development of a general approach for the biochemical functionalization of peptide nanotubes (PNTs) could open up existing opportunities in both fundamental studies as well as a variety of applications. PNTs are spontaneously assembled organic nanostructures made from peptides. Phage display has emerged as a powerful approach for identifying selective peptide binding motifs. Here, we demonstrate for the first time the biochemical functionalization of PNTs via peptides identified from a phage display peptide library. The phage-displayed peptides are shown to recognize PNTs. These advances further allow for the development of bifunctional peptides for the capture of bacteria and the self-assembly of silver particles onto PNTs. We anticipate that these results could provide significant opportunities for using PNTs in both fundamental studies and practical applications, including sensors and biosensors nanoelectronics, energy storage devices, drug delivery, and tissue engineering. PMID:27479451

  9. Dans le grand collisionneur du CERN

    CERN Document Server

    Campanelli, Mario

    2014-01-01

    Ce livre fait pénétrer le lecteur non initié dans l’instrument scientifique le plus grand du monde. D’ailleurs, le web fut précisément inventé à Genève en vue d’une collaboration aussi gigantesque. Résultat de vingt ans de recherche, le LHC, Grand collisionneur de hadrons, est l’anneau de 26,7 km creusé sous la frontière francosuisse, où sont accélérés, en sens opposés, des paquets de particules jusqu’à des vitesses et des énergies extrêmes. Ainsi la physique s’est-elle engagée dans une étape nouvelle de nos connaissances. Après les grandes découvertes de l’univers et de la réalité microscopique du siècle dernier, le collisionneur nous fait progresser aujourd’hui dans un «nuage de probabilités» aux frontières de l’antimatière. Dans une bousculade de particules, voici le champ convoité du boson de Higgs. Mario Campanelli, professeur associé à l’Université de Londres et collaborateur au projet ATLAS du LHC, propose, dans cet ouvrage, de s’introduire pas �...

  10. Experimental reproducibility analysis in DU hydriding

    Energy Technology Data Exchange (ETDEWEB)

    Koo, Daeseo; Park, Jongcheol; Chung, Hongsuk [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-10-15

    A storage and delivery system (SDS) is used for storing hydrogen isotopes as a metal hydride form. The rapid hydriding of tritium is very important not only for safety reasons but also for the economic design and operation of the SDS. For the storage, supply, and recovery of hydrogen isotopes, depleted uranium (DU) has been extensively proposed. To develop nuclear fusion technology, it will be necessary to store and supply hydrogen isotopes needed for Tokamak operation. The experimental reproducibility of bed temperature on DU hydriding was also analyzed. The experimental reproducibility of apparatus was acceptable for all the experiments. The experimental reproducibility of tank pressure on DU hydriding was analyzed. As the hydriding performs, the tank pressure showed decreasing trend. The experimental reproducibility of bed temperature on DU hydriding was also analyzed. As the hydriding performs, the bed temperatures increased up to maximum temperature with exothermic reaction and then they showed decreasing trend. The experimental reproducibility of apparatus was acceptable for all the experiments.

  11. La Carte du Ciel vue de Potsdam

    Science.gov (United States)

    Bigg, C.

    2008-06-01

    Ce chapitre s'attache à retracer les aléas de la Carte du Ciel depuis l'Observatoire de Potsdam. Le cas de cet établissement sert de point de comparaison avec les observatoires français, en particulier en ce qui concerne l'organisation du travail au sein de l'Observatoire et le problème de la répartition des ressources humaines et instrumentales dans la poursuite de ce projet de longue haleine. On remarque notamment que si les astronomes allemands, héritiers d'une tradition d'excellence en astrométrie stellaire, se devaient de prendre part à la "Photographische Himmelskarte", ce ne sont pas les observatoires d'astronomie classique mais le nouvel observatoire d'astrophysique de Potsdam qui entreprendra cette tâche. Les études effectuées dans ce cadre sur la photographie stellaire et surtout la photométrie photographique seront ainsi considérées comme des contributions à la jeune science astrophysique - du moins jusqu'à ce que la Carte du Ciel entre en concurrence dans l'Observatoire avec les projets de catalogues de spectres stellaires et de mesure des vitesses radiales.

  12. Esquisse du parlementarisme et du monopartisme en Afrique: le cas du Togo

    Directory of Open Access Journals (Sweden)

    E. A. B. van Rouveroy van Nieuwaal

    1989-01-01

    Full Text Available Witnessed the last hundred years profound political and constitutional changes. In this respect there are many differences between African States depending on which kind of colonial overlord has been the ruling power. The African State was a juridical entity in international law, but was it also, at the time of independence, an empirical entity in national fact?In almost all cases the empirical reality as a functioning government was still primarily the presence of European bureaucrats who has embodied the colonial state. Independence therefore opened a gap between the international legitimacy and the internal marginality of many emergent African State. The gap often presented a real political dilemma to the new African rulers: they usually could retain European officials only by compromising their national independence and could dispense with them only at the risk of undermining governmental performance. After adoption of European constitutional law and with, initially, a high degree of similarity between most constitutions in French-speaking Africa, nowadays these constitutions differ widely, contrary to the Commonwealth Africa that has experienced fewer innovations and constitutional breakdowns.Parliamentary systems of government gave way to One Party Systems, introduced in many cases by the national army, as the best equiped, trained, paid and organised power in the country. This has been the political and constitutional development in Togo as well. Since 1969, it has a One Party System, the Rassemblement du Peuple Togolais, founded by the President of the Togolese Republic, Gen. Gnassingbé Eyadéma. But, as everywhere else, doubts are growing about the effectiveness of the One Party System. Are the Togolese perspectives such that Eyadéma is willing to have an open mind and to be all ears for the critics to his own creation? Another intriguing question is to know in which way the Africantraditional authorities (" chieftancy", as the core

  13. Radiolabelled peptides for oncological diagnosis

    Energy Technology Data Exchange (ETDEWEB)

    Laverman, Peter; Boerman, Otto C.; Oyen, Wim J.G. [Radboud University Nijmegen Medical Centre, Department of Nuclear Medicine, Nijmegen (Netherlands); Sosabowski, Jane K. [Queen Mary University of London, Centre for Molecular Oncology, Barts Cancer Institute, London (United Kingdom)

    2012-02-15

    Radiolabelled receptor-binding peptides targeting receptors (over)expressed on tumour cells are widely under investigation for tumour diagnosis and therapy. The concept of using radiolabelled receptor-binding peptides to target receptor-expressing tissues in vivo has stimulated a large body of research in nuclear medicine. The {sup 111}In-labelled somatostatin analogue octreotide (OctreoScan trademark) is the most successful radiopeptide for tumour imaging, and was the first to be approved for diagnostic use. Based on the success of these studies, other receptor-targeting peptides such as cholecystokinin/gastrin analogues, glucagon-like peptide-1, bombesin (BN), chemokine receptor CXCR4 targeting peptides, and RGD peptides are currently under development or undergoing clinical trials. In this review, we discuss some of these peptides and their analogues, with regard to their potential for radionuclide imaging of tumours. (orig.)

  14. Biomimetic oligosaccharide and peptide surfactant polymers designed for cardiovascular biomaterials

    Science.gov (United States)

    Ruegsegger, Mark Andrew

    A common problem associated with cardiovascular devices is surface induced thrombosis initiated by the rapid, non-specific adsorption of plasma proteins onto the biomaterial surface. Control of the initial protein adsorption is crucial to achieve the desired longevity of the implanted biomaterial. The cell membrane glycocalyx acts as a non-thrombogenic interface through passive (dense oligosaccharide structures) and active (ligand/receptor interactions) mechanisms. This thesis is designed to investigate biomimicry of the cell glycocalyx to minimize non-specific protein adsorption and promote specific ligand/receptor interactions. Biomimetic macromolecules were designed through the molecular-scale engineering of polymer surfactants, utilizing a poly(vinyl amine) (PVAm) backbone to which hydrophilic (dextran, maltose, peptide) and hydrophobic alkyl (hexanoyl or hexanal) chains are simultaneously attached. The structure was controlled through the molar feed ratio of hydrophobic-to-hydrophilic groups, which also provided control of the solution and surface-active properties. To mimic passive properties, a series of oligomaltose surfactants were synthesized with increasing saccharide length (n = 2, 7, 15 where n is number of glucose units) to investigate the effect of coating height on protein adsorption. The surfactants were characterized by infra red (IR) and nuclear magnetic resonance (NMR) spectroscopies for structural properties and atomic force microscopy (AFM) and contact angle goniometry for surface activity. Protein adsorption under dynamic flow (5 dyn/cm2) was reduced by 85%--95% over the bare hydrophobic substrate; platelet adhesion dropped by ˜80% compared to glass. Peptide ligands were incorporated into the oligosaccharide surfactant to promote functional activity of the passive coating. The surfactants were synthesized to contain 0%, 25%, 50%, 75%, and 100% peptide ligand density and were stable on hydrophobic surfaces. The peptide surface density was

  15. Debromination of endo-(+)-3-bromocamphor with primary amines

    International Nuclear Information System (INIS)

    Reductive debromination of endo-(+)-3-bromocamphor with different primary amines followed by imine formation was investigated. This reaction requires simple experimental procedure without any organic solvent, metal or conventional reducing agent. A strong influence of amine polarity on the efficacy of debromination process was observed, and ethanolamine and ethylene diamine having sufficiently high boiling points can debrominate 3-bromocamphor giving corresponding camphanimines in good isolated yields. The mechanisms of debromination of 3-bromocamphor with ethanolamine and n-hexylamine were investigated at the B3LYP/6-311+G(d,p) level of theory. The radical mechanism was revealed, and it was shown that the reaction with more polar ethanolamine is energetically more favorable. (author)

  16. Debromination of endo-(+)-3-bromocamphor with primary amines

    Energy Technology Data Exchange (ETDEWEB)

    Markovic, Svetlana; Markovic, Violeta; Joksovic, Milan D.; Joksovic, Ljubinka, E-mail: ljubinka@kg.ac.rs [Department of Chemistry, Faculty of Science, University of Kragujevac (Serbia); Todorovic, Nina [Institute for Chemistry, Technology and Metallurgy, Belgrade (Serbia); Divjakovic, Vladimir [Department of Physics, University of Novi Sad (Serbia); Trifunovic, Snezana [Faculty of Chemistry, University of Belgrade, Belgrade, (Serbia)

    2013-07-15

    Reductive debromination of endo-(+)-3-bromocamphor with different primary amines followed by imine formation was investigated. This reaction requires simple experimental procedure without any organic solvent, metal or conventional reducing agent. A strong influence of amine polarity on the efficacy of debromination process was observed, and ethanolamine and ethylene diamine having sufficiently high boiling points can debrominate 3-bromocamphor giving corresponding camphanimines in good isolated yields. The mechanisms of debromination of 3-bromocamphor with ethanolamine and n-hexylamine were investigated at the B3LYP/6-311+G(d,p) level of theory. The radical mechanism was revealed, and it was shown that the reaction with more polar ethanolamine is energetically more favorable. (author)

  17. Asymmetric trienamine catalysis: new opportunities in amine catalysis.

    Science.gov (United States)

    Kumar, Indresh; Ramaraju, Panduga; Mir, Nisar A

    2013-02-01

    Amine catalysis, through HOMO-activating enamine and LUMO-activating iminium-ion formation, is receiving increasing attention among other organocatalytic strategies, for the activation of unmodified carbonyl compounds. Particularly, the HOMO-raising activation concept has been applied to the greatest number of asymmetric transformations through enamine, dienamine, and SOMO-activation strategies. Recently, trienamine catalysis, an extension of amine catalysis, has emerged as a powerful tool for synthetic chemists with a novel activation strategy for polyenals/polyenones. In this review article, we discuss the initial developments of trienamine catalysis for highly asymmetric Diels-Alder reactions with different dienophiles and emerging opportunities for other types of cycloadditions and cascade reactions.

  18. The effect of fluorine substitution in alcohol-amine complexes

    DEFF Research Database (Denmark)

    Hansen, Anne Schou; Du, Lin; Kjærgaard, Henrik Grum

    2014-01-01

    The effect of fluorine substitution on the hydrogen bond strength in alcohol-amine molecular complexes was investigated, with a combination of vapour phase infrared spectroscopy and theoretical calculations. The complexes were combined from methanol (MeOH), ethanol (EtOH) and trifluoroethanol (TFE...... formation were determined by combining a calculated intensity and the measured integrated absorbance. The observation of two transitions in the alcohol-DMA complexes provides an opportunity for two independent determinations of the equilibrium constants. Molecular interactions between the monomers were...... elucidated by Natural Bond Orbital, Atoms in Molecules and Non-covalent Interactions analysis. We find that the alcohol-amine complexes with TFE as the hydrogen bond donor form stronger hydrogen bonds and that secondary interactions between the monomers increase from MeOH to EtOH to TFE. TFE is a stronger...

  19. Optimization of amine-terminated polyacrylonitrile synthesis and characterization

    Directory of Open Access Journals (Sweden)

    Mohamed H. El-Newehy

    2014-04-01

    Full Text Available Amine-terminated PANs were prepared in two steps. The first step includes free radical polymerization of acrylonitrile (AN using initiator pair of ammonium persulfate and sodium thiosulfate as redox system. In the second step, the amino groups were introduced through the reaction of polyacrylonitrile with excess of different diamines (10-fold including ethylenediamine (EDA, hexamethylenediamine (HMDA and octamethylenediamine (OMDA, to yield PAN–EDA, PAN–HMDA and PAN–OMDA, respectively. Optimization of the amine-terminated PANs synthesis was carried out at different temperatures (30–90 °C and different time intervals (4–24 h. In addition, the introduction of the amino group was followed by the piperidine test and recording of the FT-IR spectra. All polymers were characterized by, 1H NMR spectra, thermogravimetric analysis (TGA, and FT-IR spectra.

  20. Adsorption of catechol from aqueous solution by aminated hypercrosslinked polymers

    Institute of Scientific and Technical Information of China (English)

    SUN Yue; LI Xiao-tao; XU Chao; CHEN Jin-long; LI Ai-min; ZHANG Quan-xing

    2005-01-01

    Adsorption of catechol from aqueous solution with the hypercrosslinked polymeric adsorbent NDA-100 and its derivatives AH-1,AH-2 and AH-3 aminated by dimethylamine, the commercial resin Amberlite XAD-4 and weakly basic anion exchanger resin D301 was compared. It was found that the aminated hypercrosslinked resins had the highest adsorption capacities among the tested polymers. The empirical Freundlich equation was successfully employed to describe the adsorption process. Specific surface area and micropore structure of the adsorbent, in company with tertiary amino groups on matrix affected the adsorption performance towards catechol. In addition,thermodynamic study was carried out to interpret the adsorption mechanism. Kinetic study testified that the tertiary amino groups on the polymer matrix could decrease the adsorption rate and increase the adsorption apparent activation energy.

  1. Aromatic Esters of Bicyclic Amines as Antimicrobials against Streptococcus pneumoniae.

    Science.gov (United States)

    de Gracia Retamosa, María; Díez-Martínez, Roberto; Maestro, Beatriz; García-Fernández, Esther; de Waal, Bas; Meijer, E W; García, Pedro; Sanz, Jesús M

    2015-11-01

    A double approach was followed in the search of novel inhibitors of the surface choline-binding proteins (CBPs) of Streptococcus pneumoniae (pneumococcus) with antimicrobial properties. First, a library of 49 rationally-designed esters of alkyl amines was screened for their specific binding to CBPs. The best binders, being esters of bicyclic amines (EBAs), were then tested for their in vitro effect on pneumococcal growth and morphology. Second, the efficiency of EBA-induced CBP inhibition was enhanced about 45,000-fold by multivalency effects upon synthesizing a poly(propylene imine) dendrimer containing eight copies of an atropine derivative. Both approaches led to compounds that arrest bacterial growth, dramatically decrease cell viability, and exhibit a protection effect in animal disease models, demonstrating that the pneumococcal CBPs are adequate targets for the discovery of novel antimicrobials that overcome the currently increasing antimicrobial resistance issues. PMID:26377931

  2. Metabolic activation of aromatic amines and azo dyes.

    Science.gov (United States)

    Bartsch, H

    1981-01-01

    Aromatic amines, amides and nitro compounds are a class of chemicals that produce tumors in a wide variety of tissues in experimental animals, including liver, urinary bladder, forestomach, small intestine, Zymbal's gland, subcutaneous tissue or skin. In man, exposure to some aromatic amines is associated with tumours of the urinary bladder and carcinoma of the renal pelvis. Their biological activity as carcinogens or genotoxic agents is, in all the cases that have been studied in detail, dependent on metabolic activation in vivo, occurring by multiple pathways. Differences in these metabolic pathways may largely account for the differences in tissues and species susceptibilities to cancer induction. Carcinogenicity of aromatic amines or amides is dependent on their oxidation to N-hydroxy derivatives, whilst the carcinogenicity of aromatic nitro compounds is linked to their reduction to hydroxylamines. Further conversion of the N-hydroxylamine or N-hydroxyamide to reactive intermediates can occur in several ways, which include (i) esterification of the N-hydroxy group, (ii) non-enzymic protonation of the nitrogen of the hydroxylamine and (iii) oxidation to a free radical of arylhydroxamic acids. Following generation of such reactive electrophilic intermediates in tissues or cells, macromolecular binding has been observed to nucleic acids and proteins. In many cases, arylamidated and arylaminated products are formed with nucleic acid bases; in the case of the well-studied 2-acetylaminofluorene, nucleophilic atoms of guanine are the predominant site of reaction. Relatively little is known of the structure and biological consequences of DNA adducts formed from other aromatic amines, amides or nitro compounds; more research in these directions is warranted.

  3. Azide Tripodal Dendrons from Behera's Amine and Their Clicked Dendrimers.

    Science.gov (United States)

    Barmare, Farhana; Abadjian, Marie-Caline Z; Wiener, Erik C; Grotjahn, Douglas B

    2016-08-01

    Diazo transfer reactions on Behera's amine and its next-generation analogue formed G0 and G1 azide dendrons bearing three and nine tert-butyl-protected esters, respectively. The utility of the new dendrons was demonstrated by copper-catalyzed azide-alkyne cycloaddition, with 1,3,5-triethynylbenzene, forming two novel dendrimers in a convergent manner. Acid-mediated dendrimer deprotection was successful, and the resulting carboxy-terminated dendrimers were analyzed by NMR and DOSY experiments. PMID:27454340

  4. Biogenic amines in smear and mould-ripened cheeses

    OpenAIRE

    Pavel Pleva; Leona Buňková; Eva Theimrová; Vendula Bartošáková; František Buňka; Khatantuul Purevdorj

    2014-01-01

    The aim of the study was the monitoring of six biogenic amines (histamine, tyramine, phenylethylamine, tryptamine, putrescine, and cadaverine) and two polyamines (spermidine and spermine) in 30 samples of dairy products purchased in the Czech Republic, namely in 15 samples of mould-ripened cheeses and in 15 samples of smear-ripened cheeses. A further goal was the microbiological analysis of the individual samples of cheeses (total count of microorganisms, number of enterobacteria, enterococci...

  5. Analysis of Some Biogenic Amines by Micellar Liquid Chromatography

    OpenAIRE

    Irena Malinowska; Katarzyna E. Stępnik

    2012-01-01

    Micellar liquid chromatography (MLC) with the use of high performance liquid chromatography (HPLC) was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine. In this paper, an influence of surfactant’s concentration and pH of the micellar mobile phase on the retention of the tested substances was examined. To determine the influence of surfactant’s concentration on the retention of the tested ami...

  6. [Metabolism of various biogenic amines in acute viral neuroinfections].

    Science.gov (United States)

    Martynenko, I N; Shchegoleva, R A; Ponomarenko, V P; Leshchinskaia, E V; Kodkind, G Kh

    1983-01-01

    The metabolism of biogenic amines was examined in 62 patients with various acute viral neuroinfections. The control group consisted of 57 persons. Depending on the process character and disease period variations of the levels of serotonin, 5-hydroxyindolylacetic acid, coeruloplasmin and histamine were discovered. A comparison of the results obtained with the clinical course of the diseases revealed a certain correlation, especially in patients with acute meningoencephalitis.

  7. Electrophysiological effects of trace amines on mesencephalic dopaminergic neurons

    Directory of Open Access Journals (Sweden)

    Ada eLedonne

    2011-07-01

    Full Text Available Trace amines (TAs are a class of endogenous compounds strictly related to classic monoamine neurotransmitters with regard to their structure, metabolism and tissue distribution. Although the presence of TAs in mammalian brain has been recognized for decades, until recently they were considered to be by-products of amino acid metabolism or as ‘false’ neurotransmitters. The discovery in 2001 of a new family of G protein-coupled receptors (GPCRs, namely trace amines receptors, has re-ignited interest in TAs. In particular, two members of the family, trace amine receptor 1 (TA1 and trace amine receptor 2 (TA2, were shown to be highly sensitive to these endogenous compounds. Experimental evidence suggests that TAs modulate the activity of catecholaminergic neurons and that TA dysregulation may contribute to neuropsychiatric disorders, including schizophrenia, attention deficit hyperactivity disorder, depression and Parkinson’s disease, all of which are characterised by altered monoaminergic networks. Here we review recent data concerning the electrophysiological effects of TAs on the activity of mesencephalic dopaminergic neurons. In the context of recent data obtained with TA1 receptor knockout mice, we also discuss the mechanisms by which the activation of these receptors modulates the activity of these neurons. Three important new aspects of TAs action have recently emerged: (a inhibition of firing due to increased release of dopamine; (b reduction of D2 and GABAB receptor-mediated inhibitory responses (excitatory effects due to dysinhibition; and (c a direct TA1 receptor-mediated activation of GIRK channels which produce cell membrane hyperpolarization. While the first two effects have been well documented in our laboratory, the direct activation of GIRK channels by TA1 receptors has been reported by others, but has not been seen in our laboratory (Geracitano et al., 2004. Further research is needed to address this point, and to further

  8. Preparation of mesoporous aluminophosphate usingpoly(amido amine) as template

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiaofeng; LIN Shen; CHEN Xinqing; CHEN Jiebo; YANG Liuyi; LUO Minghong

    2007-01-01

    Mesoporous aluminophosphate was prepared by using G4.0 poly(amido amine)dendrimer as a template and characterized by Fourier transform infrared spectrometer(FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and N2 adsorption/desorption methods.Results show that the title compound exhibits a typical mesoporous structure with the average pore size from 5 to 8 nm. The formation mechanism of the nanoporous structure using dendrimer as a template was also discussed.

  9. Ionic-Liquid-Supported Synthesis of Amines and Derivatives

    OpenAIRE

    Debdab, Mansour; Mongin, Florence; Bazureau, J.P.

    2006-01-01

    International audience Amine precursors such as glycines protected at nitrogen with a Boc or formyl group were grafted by esterification on the hydroxylated arms of 1-(2-hydroxyethyl)-3-methylimidazolium hexafluorophosphates or tetrafluoroborates. The cleavage of the Boc group was then realized at room temperature by successively treating acetonitrile solutions of the thus formed glycinates with methanol and acetyl chloride (two equivalents each). Interestingly, the resulting glycinate hyd...

  10. Lysine-tagged peptide coupling onto polylactide nanoparticles coated with activated ester-based amphiphilic copolymer: a route to highly peptide-functionalized biodegradable carriers.

    Science.gov (United States)

    Handké, Nadège; Ficheux, Damien; Rollet, Marion; Delair, Thierry; Mabrouk, Kamel; Bertin, Denis; Gigmes, Didier; Verrier, Bernard; Trimaille, Thomas

    2013-03-01

    Efficient biomolecule conjugation to the surface of biodegradable colloidal carriers is crucial for their targeting efficiency in drug/vaccine delivery applications. We here propose a potent strategy to drastically improve peptide immobilization on biodegradable polylactide (PLA) nanoparticles (NPs). Our approach particularly relies on the use of an amphiphilic block copolymer PLA-b-poly(N-acryloxysuccinimide-co-N-vinylpyrrolidone) (PLA-b-P(NAS-co-NVP)) as NP surface modifier, whose the N-succinimidyl (NS) ester functions of the NAS units along the polymer chain ensure N-terminal amine peptide coupling. The well-known immunostimulatory peptide sequence derived from the human interleukin 1β (IL-1β), VQGEESNDK, was coupled on the NPs of 169 nm mean diameter in phosphate buffer (pH 8, 10 mM). A maximum amount of 2 mg immobilized per gram of NPs (i.e. 0.042 peptidenm(-2)) was obtained. Introduction of a three lysine tag at the peptide N-terminus (KKKVQGEESNDK) resulted in a dramatic improvement of the immobilized peptide amounts (27.5 mg/g NP, i.e. 0.417 peptidenm(-2)). As a comparison, the density of tagged peptide achievable on surfactant free PLA NPs of similar size (140 nm), through classical EDC or EDC/NHS activation of the surface PLA carboxylic end-groups, was found to be 6 mg/g NP (i.e. 0.075 peptidenm(-2)), showing the decisive impact of the P(NAS-co-NVP)-based hairy corona for high peptide coupling. These results demonstrate that combined use of lysine tag and PLA-b-P(NAS-co-NVP) surfactant represents a valuable platform to tune and optimize surface bio-functionalization of PLA-based biodegradable carriers. PMID:23277324

  11. Bacterial fermentation platform for producing artificial aromatic amines

    Science.gov (United States)

    Masuo, Shunsuke; Zhou, Shengmin; Kaneko, Tatsuo; Takaya, Naoki

    2016-01-01

    Aromatic amines containing an aminobenzene or an aniline moiety comprise versatile natural and artificial compounds including bioactive molecules and resources for advanced materials. However, a bio-production platform has not been implemented. Here we constructed a bacterial platform for para-substituted aminobenzene relatives of aromatic amines via enzymes in an alternate shikimate pathway predicted in a Pseudomonad bacterium. Optimization of the metabolic pathway in Escherichia coli cells converted biomass glucose to 4-aminophenylalanine with high efficiency (4.4 g L−1 in fed-batch cultivation). We designed and produced artificial pathways that mimicked the fungal Ehrlich pathway in E. coli and converted 4-aminophenylalanine into 4-aminophenylethanol and 4-aminophenylacetate at 90% molar yields. Combining these conversion systems or fungal phenylalanine decarboxylases, the 4-aminophenylalanine-producing platform fermented glucose to 4-aminophenylethanol, 4-aminophenylacetate, and 4-phenylethylamine. This original bacterial platform for producing artificial aromatic amines highlights their potential as heteroatoms containing bio-based materials that can replace those derived from petroleum. PMID:27167511

  12. DFT investigation on dihydrogen-bonded amine-borane complexes.

    Science.gov (United States)

    Yan, Shihai; Zou, Hongmei; Kang, Wukui; Sun, Lixiang

    2016-01-01

    The DFT method has been employed in the exploration on dihydrogen-bonded amine-borane complexes, with a special emphasis on the dimerization and substituent group effect. Stable dihydrogen bonded complexes can be generated from these amine-borane monomers with the appearance of NH(δ+)…H(δ-)B interactions. The binding energy decreases gradually with the increase of the steric effect of the substituents. The substituent group number mainly varies the C-N bond length. The dimerization generates close H…H and influences predominantly the N-B distance. The effect of dimerization on IR and vibrational circular dichroism (VCD) spectra is stronger than that of the number of substituent groups, which leads to distinct NBO charge variation on α-C. Both the substituent group number and dimerization enhance the chemical shift difference between hydrogen atoms covalently bonded to N and B, Δδ H-H, which can be hired as an index for structural determination. It is proposed that amine-borane complexes with more substituent groups in higher degree of polymerization are potentially interesting materials for hydrogen storage. Graphical Abstract Both the number of substituent group and dimerization enhance the chemical shift difference of hydrogen atoms covalently bonded on N and B, Δδ H-H, which can be employed as an index for the structural determination.

  13. Bioactive amines and internal quality of commercial eggs.

    Science.gov (United States)

    Figueiredo, T C; Viegas, R P; Lara, L J C; Baião, N C; Souza, M R; Heneine, L G D; Cançado, S V

    2013-05-01

    The physicochemical and microbiological qualities of commercial eggs produced by layer hens of different ages (approximately 30 and 60 wk old) were submitted to storage under room temperature or refrigeration for 28 d. A total of 600 eggs were subjected to microbiological analyses of their inner contents and another 600 to a determination of Haugh units (HU) and bioactive amine content. A decrease in the quality of the inner contents of the eggs was observed during the experiment, mainly in the eggs from the 60-wk-old layers, which presented the worst HU values when stored at room temperature (P Pseudomonas spp., mesophilic aerobic bacteria, and fungi were also recorded. The chromatographic analysis of bioactive amines detected the presence of phenylethylamine in all albumens (38.0 mg/kg) and spermidine in the yolks (1.02 mg/kg). It was concluded that the age of the hens and the time and temperature of storage influenced the quality parameters of the eggs (P < 0.05). Furthermore, despite the low levels of microbial contamination found, phenylethylamine was detected in the albumen. It was not possible to establish index of quality of eggs using bioactive amines present in the yolk and albumen of eggs. PMID:23571349

  14. Amine-functionalized polypyrrole: Inherently cell adhesive conducting polymer.

    Science.gov (United States)

    Lee, Jae Y; Schmidt, Christine E

    2015-06-01

    Electrically conducting polymers (CPs) have been recognized as novel biomaterials that can electrically communicate with biological systems. For their tissue engineering applications, CPs have been modified to promote cell adhesion for improved interactions between biomaterials and cells/tissues. Conventional approaches to improve cell adhesion involve the surface modification of CPs with biomolecules, such as physical adsorption of cell adhesive proteins and polycationic polymers, or their chemical immobilization; however, these approaches require additional multiple modification steps with expensive biomolecules. In this study, as a simple and effective alternative to such additional biomolecule treatment, we synthesized amine-functionalized polypyrrole (APPy) that inherently presents cell adhesion-supporting positive charges under physiological conditions. The synthesized APPy provides electrical activity in a moderate range and a hydrophilic surface compared to regular polypyrrole (PPy) homopolymers. Under both serum and serum-free conditions, APPy exhibited superior attachment of human dermal fibroblasts and Schwann cells compared to PPy homopolymer controls. Moreover, Schwann cell adhesion onto the APPy copolymer was at least similar to that on poly-l-lysine treated PPy controls. Our results indicate that amine-functionalized CP substrates will be useful to achieve good cell adhesion and potentially electrically stimulate various cells. In addition, amine functionality present on CPs can further serve as a novel and flexible platform to chemically tether various bioactive molecules, such as growth factors, antibodies, and chemical drugs. PMID:25294089

  15. Antimicrobial Peptides (AMPs

    Directory of Open Access Journals (Sweden)

    Mehrzad Sadredinamin

    2016-04-01

    Full Text Available Antimicrobial peptides (AMPs are extensive group of molecules that produced by variety tissues of invertebrate, plants, and animal species which play an important role in their immunity response. AMPs have different classifications such as; biosynthetic machines, biological sources, biological functions, molecular properties, covalent bonding patterns, three dimensional structures, and molecular targets.These molecules have multidimensional properties including antimicrobial activity, antiviral activity, antifungal activity, anti-parasite activity, biofilm control, antitumor activity, mitogens activity and linking innate to adaptive immunity that making them promising agents for therapeutic drugs. In spite of this advantage of AMPs, their clinical developments have some limitation for commercial development. But some of AMPs are under clinical trials for the therapeutic purpose such as diabetic foot ulcers, different bacterial infections and tissue damage. In this review, we emphasized on the source, structure, multidimensional properties, limitation and therapeutic applications of various antimicrobial peptides.

  16. Antimicrobial peptides in Echinoderms

    Directory of Open Access Journals (Sweden)

    C Li

    2010-05-01

    Full Text Available Antimicrobial peptides (AMPs are important immune effector molecules for invertebrates, including echinoderms, which lack a vertebrate-type adaptive immune system. Here we summarize the knowledge of such peptides in echinoderms. Strongylocins are a novel family of cysteine-rich AMPs, recently identified in the sea urchins, Strongylocentrotus droebachiensis and S. purpuratus. Although these molecules present diverse amino acid sequences, they share an identical cysteine arrangement pattern, dissimilar to other known AMPs. A family of heterodimeric AMPs, named centrocins, are also present in S. droebachiensis. Lysozymes and fragments of larger proteins, such as beta-thymocins, actin, histone 2A and filamin A have also been shown to display antimicrobial activities in echinoderms. Future studies on AMPs should be aimed in revealing how echinoderms use these AMPs in the immune response against microbial pathogens.

  17. Equilibrium and Transport Properties of Primary, Secondary and Tertiary Amines by Molecular Simulation

    Directory of Open Access Journals (Sweden)

    Orozco Gustavo A.

    2014-09-01

    Full Text Available Using molecular simulation techniques such as Monte Carlo (MC and molecular dynamics (MD, we present several simulation results of thermodynamic and transport properties for primary, secondary and tertiary amines. These calculations are based on a recently proposed force field for amines that follows the Anisotropic United Atom approach (AUA. Different amine molecules have been studied, including n-ButylAmine, di-n-ButylAmine, tri-n-ButylAmine and 1,4-ButaneDiAmine for primary, secondary, tertiary and multi-functional amines respectively. For the transport properties, we have calculated the viscosity coefficients as a function of temperature using the isothermal-isobaric (NPT ensemble. In the case of the pure components, we have investigated different thermodynamic properties using NVT Gibbs ensemble simulations such as liquid-vapor phase equilibrium diagrams, vaporization enthalpies, vapor pressures, normal boiling points, critical temperatures and critical densities. We have also calculated the excess enthalpies for water-n-ButylAmine and n-heptane-n-ButylAmine mixtures using Monte Carlo simulations in the NPT ensemble. In addition, we present the calculation of liquid-vapor surface tensions of n-ButylAmine using a two-phase NVT simulation as well as the radial distribution functions. Finally, we have investigated the physical Henry constants of nitrous oxide (N2O and nitrogen (N2 in an aqueous solutions of n-ButylAmine. In general, we found a good agreement between the available experimental information and our simulation results for all the studied properties, ratifying the predictive capability of the AUA force field for amines.

  18. A Review: Microbiological, Physicochemical and Health Impact of High Level of Biogenic Amines in Fish Sauce

    Directory of Open Access Journals (Sweden)

    Muhammad Z. Zaman

    2009-01-01

    Full Text Available Problem statement: Biogenic amines are basic nitrogenous compounds present in a wide variety of foods and beverages. Their formations were mainly due to the amino acids decarboxylase activity of certain microorganisms. Excessive intake of biogenic amines could induce many undesirable physiological effects determined by their psychoactive and vasoactive action. Fish sauce which is considered as a good source of dietary protein, amino acids, vitamins and minerals was a popular condiment in Southeast Asian countries. However, it has also been reported that fish sauce contain high amount of amines. Hence, attention should be given to ensure the safety of this product. Approach: A review study was conducted to deliver an overview on the presence of biogenic amines in fish sauce and to discuss the important factors affecting their accumulation. Impact of amines on human health and efforts to reduce their accumulation in fish sauce were also discussed to give a comprehensive view. Results: Histamine, putrescine and cadaverine is the most abundant amines in fish sauce with maximum reported value of 1220, 1257 and 1429 ppm, respectively. Tyramine present in a lesser amount with maximum reported value of 1178 ppm. Other amines such as tryptamine, phenylethylamine, spermine and spermidine were considered as minor amines. However, different profiles of amines were reported in different type of products. This was depended on microbial flora, availability of precursors and physicochemical factors such as temperature, pH, salt, oxygen and sugar concentration. In synergistically supporting physicochemical factors, several microorganisms such as Enterobacteriaceae, Micrococci and Lactobacilli were responsible for biogenic amines formation in fish sauce. Conclusion: Since the formation of amines in fish sauce was a result of many factors, it was almost virtually impossible to control each factor during fermentation. Addition of amines degrading bacteria into fish

  19. Solid-Phase Synthetic Route to Multiple Derivatives of a Fundamental Peptide Unit

    Directory of Open Access Journals (Sweden)

    Martin J. O’Donnell

    2010-07-01

    Full Text Available Amino acids are Nature’s combinatorial building blocks. When substituted on both the amino and carboxyl sides they become the basic scaffold present in all peptides and proteins. We report a solid-phase synthetic route to large combinatorial variations of this fundamental scaffold, extending the variety of substituted biomimetic molecules available to successfully implement the Distributed Drug Discovery (D3 project. In a single solid-phase sequence, compatible with basic amine substituents, three-point variation is performed at the amino acid a-carbon and the amino and carboxyl functionalities.

  20. From caffeine to fish waste: amine compounds present in food and drugs and their interactions with primary amine oxidase.

    LENUS (Irish Health Repository)

    Olivieri, Aldo

    2011-07-01

    Tissue bound primary amine oxidase (PrAO) and its circulating plasma-soluble form are involved, through their catalytic activity, in important cellular roles, including the adhesion of lymphocytes to endothelial cells during various inflammatory conditions, the regulation of cell growth and maturation, extracellular matrix deposition and maturation and glucose transport. PrAO catalyses the oxidative deamination of several xenobiotics and has been linked to vascular toxicity, due to the generation of cytotoxic aldehydes. In this study, a series of amines and aldehydes contained in food and drugs were tested via a high-throughput assay as potential substrates or inhibitors of bovine plasma PrAO. Although none of the compounds analyzed were found to be substrates for the enzyme, a series of molecules, including caffeine, the antidiabetics phenformin and tolbutamide and the antimicrobial pentamidine, were identified as PrAO inhibitors. Although the inhibition observed was in the millimolar and micromolar range, these data show that further work will be necessary to elucidate whether the interaction of ingested biogenic or xenobiotic amines with PrAO might adversely affect its biological roles.

  1. Présentation du volume

    Directory of Open Access Journals (Sweden)

    Ana Zwitter Vitez

    2012-12-01

    Full Text Available Dans de nombreuses disciplines scientifiques, le discours parlé représente depuis quelques décennies un objet de recherche proéminent. Ce fait s’explique probablement par deux caractéristiques principales du discours parlé: la structure complexe de sa matérialité et les fonctions variées de différentes pratiques langagières. C’est pourquoi le présent numéro réunit les articles basés sur les approches actuelles du discours parlé et sur les pratiques langagières spécifiques. Le volume est introduit par deux articles interdisciplinaires: Peter Garrard et Ahmed Samrah présentent le domaine de la pathologie du langage en donnant une revue fouillée sur les approches actuelles de l’analyse linguistique lors de la détection de la maladie d’Alzheimer, tandis que Harry Hollien donne un aperçu structuré sur le domaine de la phonétique judiciaire destiné à dévoiler l’identité des locuteurs et de détecter la déception dans le discours parlé. Les analyses explorant l’acquisition du langage commencent par l’article de Katharina Zipser qui examine la progression de structures grammaticales en les mettant en comparaison avec la compétence des apprenants et continuent par l’étude de Meta Lah proposant une évaluation des documents audiovisuels proposés aux apprenants de langue étrangère. Vesna Požgaj Hadži, Damir Horga et Tatjana Balažic Bulc remettent en question la corrélation entre la compétence linguistique et la fluence linguistique auprès de locuteurs non-maternels et l'analyse de Gemma Santiago Alonso aborde l'acquisition de l'article défini dans le langage enfantin. Les articles suivants se réunissent autour du domaine de l'interprétation: Jana Zidar Forte présente une approche actuelle dans l'entrainement d'interprètes, Lea Burjan analyse les phénomènes issus de la pratique de l'interprétation juridique et Simona  Šumrada traite de la reformulation dans le discours de la traduction et de

  2. Selecting protein N-terminal peptides by combined fractional diagonal chromatography.

    Science.gov (United States)

    Staes, An; Impens, Francis; Van Damme, Petra; Ruttens, Bart; Goethals, Marc; Demol, Hans; Timmerman, Evy; Vandekerckhove, Joël; Gevaert, Kris

    2011-07-14

    In recent years, procedures for selecting the N-terminal peptides of proteins with analysis by mass spectrometry have been established to characterize protease-mediated cleavage and protein α-N-acetylation on a proteomic level. As a pioneering technology, N-terminal combined fractional diagonal chromatography (COFRADIC) has been used in numerous studies in which these protein modifications were investigated. Derivatization of primary amines--which can include stable isotope labeling--occurs before trypsin digestion so that cleavage occurs after arginine residues. Strong cation exchange (SCX) chromatography results in the removal of most of the internal peptides. Diagonal, reversed-phase peptide chromatography, in which the two runs are separated by reaction with 2,4,6-trinitrobenzenesulfonic acid, results in the removal of the C-terminal peptides and remaining internal peptides and the fractionation of the sample. We describe here the fully matured N-terminal COFRADIC protocol as it is currently routinely used, including the most substantial improvements (including treatment with glutamine cyclotransferase and pyroglutamyl aminopeptidase to remove pyroglutamate before SCX, and a sample pooling scheme to reduce the overall number of liquid chromatography-tandem mass spectrometry analyses) that were made since its original publication. Completion of the N-terminal COFRADIC procedure takes ~5 d.

  3. Syntheses and study of pyrrolidinic nitroxide free radicals. Preparation of a nitroxide-type stable bi-radical; Synthese et etudes de radicaux libres nitroxydes pyrrolidiniques. Preparation d'un biradical stable du type nitroxyde

    Energy Technology Data Exchange (ETDEWEB)

    Dupeyre, R.M. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires, Laboratoire de chimie organique physique

    1967-12-01

    Syntheses and study of pyrrolidinic nitroxide free radicals: These radicals are obtained by oxidation, with hydrogen peroxide, of pyrrolidinic amines prepared from triacetonamine by ring contraction. The U. V,, I. R, and E.P.R. spectral characteristics have been determined. The oxidation of these amines with hydrogen peroxide has led also to rupture of the pentagonal ring; some of the decomposition products have been identified. The high chemical stability of the nitroxide group has made it possible to synthesize and study a stable bi-radical. (author) [French] Syntheses et etudes de radicaux libres nitroxydes pyrrolidiniques: Ces radicaux sont obtenus par oxydation l'eau oxygenee d'amines pyrrolidiniques preparees a partir de la triacetonamine par contraction de cycle. Les caracteristiques spectroscopiques ultra-violettes, infra-rouge et resonance paramagnetique sont determinees. Cependant, 1'oxydation de ces amines a l'eau oxygenee a entraine la coupure du cycle pentagonal et identification de certaines substances de decomposition obtenues. La grande stabilite chimique de groupement nitroxyde a permis la synthese et l'etude d'un biradical stable. (auteur)

  4. Disassembler mezikódu jazyka Java

    OpenAIRE

    Macháček, Ondřej

    2013-01-01

    Tato práce se zabývá popisem struktury mezikódu jazyka Java a disassemblováním instrukcí mezikódu jazyka Java. Součástí této práce je knihovna pro disassemblování souborů tříd jazyka Java. Knihovna umožňuje zobrazit veškeré disassemblované informace uložené v souboru tříd. Pro ukázku práce s knihovnou byla napsána i jednoduchá aplikace s grafickým uživatelským rozhraním. This thesis focus on the structure of Java class file and disassembling bytecode instructions of Java language. Part of ...

  5. Le meurtre du prêtre

    OpenAIRE

    Clayer, Nathalie

    2010-01-01

    En septembre 1906, une çeta (bande) albanaise assassine le métropolite orthodoxe de Korçë, ville située à l'ouest de Bitola, dans une région fortement albanophone. A première vue, on pourrait voir dans ce meurtre du chef local de la communauté orthodoxe perpétré par un groupe formé en majeure partie de musulmans la manifestation tangible d'un affrontement religieux. En réalité, cet acte de violence, replacé dans un cadre plus général, doit être interprété sur un autre plan, celui du nationali...

  6. Musique ! : du phonographe au MP3

    OpenAIRE

    Tournès, Ludovic

    2011-01-01

    nouvelle édition revue et augmentée de "Du phonographe au MP3. Une histoire de la musique enregistrée, XIXe-XXIe siècle" [2008] Depuis l'invention du phonographe par Thomas Edison en 1877, jusqu'à la révolution Internet, la musique enregistrée a connu une histoire longue et riche en rebondissements que l'on trouvera retracée de manière claire, synthétique et vivante dans ce livre. A l'heure où l'industrie de la musique est engagée depuis le début des années 2000 dans une mutation historiqu...

  7. DU-AGG pilot plant design study

    International Nuclear Information System (INIS)

    The Idaho National Engineering Laboratory (INEL) is developing new methods to produce high-density aggregate (artificial rock) primarily consisting of depleted uranium oxide. The objective is to develop a low-cost method whereby uranium oxide powder (UO[sub 2], U[sub 3]O[sub ]8, or UO[sub 3]) can be processed to produce high-density aggregate pieces (DU-AGG) having physical properties suitable for disposal in low-level radioactive disposal facilities or for use as a component of high-density concrete used as shielding for radioactive materials. A commercial company, G-M Systems, conducted a design study for a manufacturing pilot plant to process DU-AGG. The results of that study are included and summarized in this report. Also explained are design considerations, equipment capacities, the equipment list, system operation, layout of equipment in the plant, cost estimates, and the proposed plan and schedule

  8. Approches Somatiques du Corps dans la Danse

    Directory of Open Access Journals (Sweden)

    Marcilio Souza

    2014-12-01

    Full Text Available L’éducation somatique est comprise comme un domaine interdisciplinaire qui porte sur la prise de conscience du corps et de son mouvement, proposant une découverte personnelle de ses propres mouvements, de ses propres sensations. Cet article vise une réflexion à travers quelques notes sur le concept d’éducation somatique, de Soma et de corps, ainsi qu’à mettre en évidence deux approches somatiques incorporées dans les pratiques du sujet en question comme les moyens possibles pour penser ce champ d’étude en danse. L’article part d’une approche phénoménologique fondée sur les études de Merleau-Ponty (1999, en prenant comme technique de recherche la description merleau-pontienne.

  9. Les danses du monde. Exemples musicaux

    OpenAIRE

    Giurchescu, Anca

    2011-01-01

    En publiant son coffret Les danses du monde, Hugo Zemp s’est attelé à la tâche périlleuse de fournir un maximum d’informations sur la danse, mode visuel d’expression humaine intimement lié à la musique. Gravitant autour de la relation étroite et compliquée qui unit la musique à la danse, les exemples parviennent à illustrer de façon équilibrée la grande diversité de l’expression vocale et instrumentale, sa richesse rythmique et dynamique, ainsi que la variété des formes de la danse et du voca...

  10. Conceptualisation statistique du champ de la culture

    OpenAIRE

    Deroin, Valérie; Chaintreau, Jean-François

    2015-01-01

    La définition du champ statistique de la culture a fait l’objet de travaux européens de 2009 à 2011, après que les nomenclatures nationales (nomenclature d’activités françaises, NAF Rév. 2) et européenne (nomenclature statistique des activités économiques dans la Communauté européenne, NACE Rév. 2) ont été rénovées en 2008. Sous l’égide d’Eurostat, l’Office statistique de l’Union européenne, un groupe de travail européen (Task Force 1) a conçu la structure du nouveau cadre statistique europée...

  11. [Analysis of amines in water samples by high performance liquid chromatography-laser induced fluorescence detection].

    Science.gov (United States)

    Liu, Fan; Gao, Fangyuan; Tang, Tao; Sun, Yuanshe; Li, Tong; Zhang, Weibing

    2013-11-01

    A sensitive high performance liquid chromatography (HPLC)-laser induced fluorescence detection (LIFD) method was developed for the determination of amines. The derivatization and separation conditions were investigated. Under the optimized conditions, spermidine, putrescine and histamine were analyzed. The limits of detection (LODs) of the three biogenic amines (S/N = 3) were as low as 10(-10) mol/L. This method showed excellent stability. The RSDs of retention times and peak areas of the three biogenic amines were lower than 0.3% and 3%, respectively. This method was applied in biogenic amine analysis in water samples, and the average recoveries were in the range of 94.99%-104.7%. Furthermore, the amines in seven tea samples were analyzed by this method, and satisfactory results were achieved. The developed assay is of excellent sensitivity and good reproducibility, which can be used in the analysis of the amines in water samples. PMID:24558849

  12. Biogenic amines in commercially produced Yulu, a Chinese fermented fish sauce.

    Science.gov (United States)

    Jiang, Wei; Xu, Ying; Li, Chunsheng; Dong, Xiaoyan; Wang, Dongfeng

    2014-01-01

    Seven biogenic amines were determined in 35 commercially produced Yulu samples from three provinces of China by pre-column derivatisation with dansyl chloride (Dns-Cl) and high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Putrescine, cadaverine, histamine and tyramine were the major biogenic amines (more than 100 mg kg(-1)), while tryptamine, spermidine and spermine were regarded as minor biogenic amines (less than 25 mg kg(-1)). Twenty samples contained more than 50 mg kg(-1) histamine (the limit for histamine in seafood products as suggested by the Food and Drug Administration). Twenty-one samples contained more than 100 mg kg(-1) tyramine and 10 contained more than 1000 mg kg(-1) total biogenic amines. This study provided data on biogenic amine levels in Chinese fermented fish sauce. The results suggested that biogenic amine content should be monitored in commercially produced Yulu. PMID:24779975

  13. Sponges with covalently tethered amines for high-efficiency carbon capture

    KAUST Repository

    Qi, Genggeng

    2014-12-12

    © 2014 Macmillan Publishers Limited. All rights reserved. Adsorption using solid amine sorbents is an attractive emerging technology for energy-efficient carbon capture. Current syntheses for solid amine sorbents mainly based on physical impregnation or grafting-to methods (for example, aminosilane-grafting) lead to limited sorbent performance in terms of stability and working capacity, respectively. Here we report a family of solid amine sorbents using a grafting-from synthesis approach and synthesized by cationic polymerization of oxazolines on mesoporous silica. The sorbent with high amount of covalently tethered amines shows fast adsorption rate, high amine efficiency and sorbent capacity well exceeding the highest value reported to date for lowerature carbon dioxide sorbents under simulated flue gas conditions. The demonstrated efficiency of the new amine-immobilization chemistry may open up new avenues in the development of advanced carbon dioxide sorbents, as well as other nitrogen-functionalized systems.

  14. Du polymorphisme au complexe d'especes : combien d'agents pathogenes sont impliques dans la necrose du collet du colza ?

    OpenAIRE

    Rouxel, Thierry; Gall, C.; Balesdent, M.H.

    1994-01-01

    La nécrose du collet des crucifères, ou «phoma du colza», est une maladie qui a subi une extension considérable à la suite de l’intensification de la culture du colza en Europe, au Canada ou en Australie. Phoma lingam (Tode ex Fr) Desm est reconnu depuis de nombreuses années comme étant l’agent responsable de la maladie. La forme sexuée du parasite, phase quasi systématique du cycle, a été identifiée dans les années 1960 à Leptosphaeria maculans (Desm) Ces et de Not. Dès 1927, 2 groupes de so...

  15. Du Pont Classifications of 6 Supernovae

    Science.gov (United States)

    Morrell, N.; Shappee, Benjamin J.

    2016-06-01

    We report optical spectroscopy (range 370-910 nm) of six supernovae from the Backyard Observatory Supernova Search (BOSS) and the All-Sky Automated Survey for Supernovae (ASAS-SN) using the du Pont 2.5-m telescope (+ WFCCD) at Las Campanas Observatory on June 17 2016 UT. We performed a cross-correlation with a library of supernova spectra using the "Supernova Identification" code (SNID; Blondin and Tonry 2007, Ap.J.

  16. Modos de ver. Du Bois y Fanon

    OpenAIRE

    Sandro Mezzadra

    2014-01-01

    The sovereignty of glance and the primacy of sight represent the Western modern culture far beyond its artistic expression, organizing its philosophical and political conceptuality. They deeply influence the way in which subjects are conceived and constructed in Western modernity. This is the point of departure of the long-distance dialogue between W. E. B. Du Bois and Frantz Fanon staged in the article. Their political thought and militancy developed against the background of experiences – s...

  17. Sådan forebygger du faldulykker

    DEFF Research Database (Denmark)

    Evron, Lotte

    2015-01-01

    Bidrag til serien 'Eksperten skriver'. Her gennemgås en kort vejledning til, hvordan man arbejder systematisk med faldforebyggelse. Ved at arbejde systematisk med dine hverdagsobservationer kan du få øje på og reagere på ændringer hos svækkede ældre borgere og på den måde forebygge faldet, før de...

  18. Autour du paradigme d’écotourisme

    Directory of Open Access Journals (Sweden)

    Anne-Laure Transler

    2008-02-01

    Full Text Available L’écotourisme ouvre un cadre conceptuel original qui permet d’envisager des démarches renouvelées de développement à partir des revenus issus de consommations touristiques mieux réfléchies et organisées, susceptibles de soutenir des initiatives de développement local s’inscrivant dans un projet global de développement durable. Variante douce du tourisme, loin du tourisme de masse, le champ de l’écotourisme s’étend aux confins du tourisme de nature, du tourisme culturel et des pratiques scientifiques et sportives de plein air. Une définition de l’écotourisme peut être proposée à partir des six points suivants : 1/ nature et culture, 2/ le bien-être des sociétés hôtes, 3/ des touristes responsables, 4/ la participation des sociétés hôtes, 5/ la durabilité, 6/ l’art de la rencontre.Ecotourism provides an original conceptual framework that enables approaches to development that are better able to provide a more equitable distribution of income, as well as better organized and thought through initiatives which are more likely to promote a holistic sustainable local development. With somewhat less negative impact than normal tourism, considerably less that mass tourism, ecotourism can encompass nature tourism, cultural tourism, scientific exploration, and even outdoor sports... A definition of ecotourism should take into account the following six points: 1/ nature and culture, 2/ the welfare of the host community, tourists acting responsibly, 3/ participation by the local community, 5 / sustainability , 6/ promotion of cultural exchanges

  19. Pourfour Du Petit syndrome after interscalene block

    Directory of Open Access Journals (Sweden)

    Mysore Chandramouli Basappji Santhosh

    2013-01-01

    Full Text Available Interscalene block is commonly associated with reversible ipsilateral phrenic nerve block, recurrent laryngeal nerve block, and cervical sympathetic plexus block, presenting as Horner′s syndrome. We report a very rare Pourfour Du Petit syndrome which has a clinical presentation opposite to that of Horner′s syndrome in a 24-year-old male who was given interscalene block for open reduction and internal fixation of fracture upper third shaft of left humerus.

  20. Sustainable growth, the DuPont way.

    Science.gov (United States)

    Holliday, C

    2001-09-01

    Like many manufacturers, DuPont traditionally has grown by making more and more "stuff." And its business growth has been proportional to the amount of raw materials and energy used--as well as the resulting waste and emissions from operations. Over the years, though, DuPont became aware that cheap supplies of nonrenewable resources wouldn't be endlessly available and that the earth's ecosystems couldn't indefinitely absorb the waste and emissions of production and consumption. Chad Holliday, chairman and CEO of DuPont, believes strongly in the challenge of sustainable growth and makes the business case for it: By using creativity and scientific knowledge effectively, he says, companies can provide strong returns for shareholders and grow their businesses--while also meeting the human needs of societies around the world and reducing the environmental footprint of their operations and products. In fact, a focus on sustainability can help identify new products, markets, partnerships, and intellectual property and lead to substantial business growth. Holliday describes how DuPont developed a three-pronged strategy to translate the concept of sustainability into nuts-and-bolts business practices. Focusing on integrated science, knowledge intensity, and productivity improvement, the strategy was accompanied by a new way to measure progress quantitatively. Sustainable growth should be viewed not as a program for stepped-up environmental performance but as a comprehensive way of doing business, one that delivers tremendous economic value and opens up new opportunities. Ultimately, companies will find that they can generate substantial business value through sustainability while both enhancing the quality of life around the world and protecting the environment.

  1. DuPont Chemical Vapor Technical Report

    International Nuclear Information System (INIS)

    DuPont Safety Resources was tasked with reviewing the current chemical vapor control practices and providing preventive recommendations on best commercial techniques to control worker exposures. The increased focus of the tank closure project to meet the 2024 Tri-Party Agreement (TPA) milestones has surfaced concerns among some CH2MHill employees and other interested parties. CH2MHill is committed to providing a safe working environment for employees and desires to safely manage the tank farm operations using appropriate control measures. To address worker concerns, CH2MHill has chartered a ''Chemical Vapors Project'' to integrate the activities of multiple CH2MHill project teams, and solicit the expertise of external resources, including an independent Industrial Hygiene expert panel, a communications consultant, and DuPont Safety Resources. Over a three-month time period, DuPont worked with CH2MHill ESH and Q, Industrial Hygiene, Engineering, and the independent expert panel to perform the assessment. The process included overview presentations, formal interviews, informal discussions, documentation review, and literature review. DuPont Safety Resources concluded that it is highly unlikely that workers in the tank farms are exposed to chemicals above established standards. Additionally, the conventional and radiological chemistry is understood, the inherent chemical hazards are known, and the risk associated with chemical vapor exposure is properly managed. The assessment highlighted management's commitment to addressing chemical vapor hazards and controlling the associated risks. Additionally, we found the Industrial Hygiene staff to be technically competent and well motivated. The tank characterization data resides in a comprehensive database containing the tank chemical compositions and relevant airborne concentrations

  2. The PeptideAtlas Project

    OpenAIRE

    Deutsch, Eric W.

    2010-01-01

    PeptideAtlas is a multi-species compendium of peptides observed with tandem mass spectrometry methods. Raw mass spectrometer output files are collected from the community and reprocessed through a uniform analysis and validation pipeline that continues to advance. The results are loaded into a database and the information derived from the raw data is returned to the community via several web-based data exploration tools. The PeptideAtlas resource is useful for experiment planning, improving g...

  3. Human Antimicrobial Peptides and Proteins

    OpenAIRE

    Guangshun Wang

    2014-01-01

    As the key components of innate immunity, human host defense antimicrobial peptides and proteins (AMPs) play a critical role in warding off invading microbial pathogens. In addition, AMPs can possess other biological functions such as apoptosis, wound healing, and immune modulation. This article provides an overview on the identification, activity, 3D structure, and mechanism of action of human AMPs selected from the antimicrobial peptide database. Over 100 such peptides have been identified ...

  4. Tribunal administratif de l'Organisation internationale du Travail (TAOIT)

    CERN Document Server

    International Labour Organization. Geneva

    Le Tribunal administratif de l'Organisation internationale du Travail connaît des requêtes formées par les fonctionnaires ou les anciens fonctionnaires du Bureau international du Travail et des quelque trente organisations qui ont reconnu sa compétence. The ILO Administrative Tribunal (ILOAT) hears complaints from serving and former officials of the International Labour Office or one of the thirty-odd intergovernmental organizations that recognise its jurisdiction.

  5. Rapport d'Activite du Service Medical - 2001/2002

    CERN Document Server

    Fassnacht, V

    2003-01-01

    Le rapport annuel de l’année 2002 fait état de l’activité du service médical et de ses conclusions en matière de santé au travail ou de conditions de travail pour les membres du personnel du CERN, c’est à dire!: les titulaires, boursiers, étudiants, utilisateurs, attachés, etc…

  6. Electron beam induced electronic transport in alkyl amine-intercalated VOx nanotubes

    OpenAIRE

    O'Dwyer, Colm; Lavayen, Vladimir; Clavijo-Cedeno, C.; Sotomayor Torres, Clivia M.

    2008-01-01

    The electron beam induced electronic transport in primary alkyl amine-intercalated V2O5 nanotubes is investigated where the organic amine molecules are employed as molecular conductive wires to an aminosilanized substrate surface and contacted to Au interdigitated electrode contacts. The results demonstrate that the high conductivity of the nanotubes is related to the non-resonant tunnelling through the amine molecules and a reduced polaron hopping conduction through the vanadium oxide itself...

  7. Tri-n-butylphosphane catalyzed ring opening of aziridines with secondary amines

    Institute of Scientific and Technical Information of China (English)

    Wan Xuan Zhang; Li Su; Wei Gang Hu; Jie Zhou

    2012-01-01

    Ring opening of aziridine with dialkyl amine took place readily in the presence of catalytic amounts of tri-n-butylphosphane (10 mol%) in the mixture of CH3CN/H2O (10:1),giving corresponding vicinal diamines in mediate to high yields (58-95%) with good regioselecfivifie,while aromatic secondary amine could not react under the same condifions.Tti-n-butylphosphane exhibited different catalytic selectivity to amines from Lewis acid catalysts.

  8. Molecular methods for the detection of biogenic amine-producing bacteria on foods

    OpenAIRE

    Landete, José María; Rivas, Blanca de las; Marcobal, Angela; Muñoz, Rosario

    2007-01-01

    Biogenic amines are low molecular weight organic bases that can be detected in raw and processed foods. Several toxicological problems resulting from the ingestion of food containing biogenic amines have been described. Biogenic amines are mainly produced by the decarboxylation of certain amino acids by microbial action. Since the ability of microorganisms to decarboxylate amino acid is highly variable, being in most cases strain-specific, the detection of bacteria possessing amino a...

  9. Analysis of Biogenic Amines by GC/FID and GC/MS

    OpenAIRE

    Nakovich, Laura

    2003-01-01

    Low levels of biogenic amines occur naturally, but high levels (FDA sets 50 ppm of histamine in fish as the maximum allowable level) can lead to scombroid poisoning. Amines in general are difficult to analyze by Gas Chromatography (GC) due to their lack of volatility and their interaction with the GC column, often leading to significant tailing and poor reproducibility. Biogenic amines need to be derivatized before both GC and HPLC analyses. The objective of this research was to devel...

  10. Improved multiplex-PCR method for the simultaneous detection of food bacteria producing biogenic amines

    OpenAIRE

    Marcobal, Ángela; Rivas, Blanca de las; Muñoz, Rosario

    2006-01-01

    The presence of biogenic amines in foods is of considerable public concern for the food industry and the regulatory agencies, since given the potential health hazard, there is a growing demand from consumers and control authorities to reduce the allowable limits of biogenic amines in foods and beverages. Rapid and simple methods are needed for the analysis of the ability to form biogenic amines by bacteria in order to evaluate the potential risk of bacterial occurring in ...

  11. Biogenic amines degradation by malolactic bacteria: towards a potential application in wine

    OpenAIRE

    GiuseppeSpano; VittorioCapozzi; Alvarez, Miguel A.; DanielaFiocco; FrancescoGrieco

    2012-01-01

    Biogenic amines in wine represent a toxicological risk for the health of the consumer, with several trade implications. In this study 26 strains of Lactobacillus plantarum were analysed for their ability to degrade biogenic amines commonly found during wine fermentation. Two strains of L. plantarum were selected in reason of their ability to degrade putrescine and tyramine. The degradation was assessed in vitro, both in presence of the biogenic amines and in presence of the specific chemical ...

  12. Biogenic amines degradation by Lactobacillus plantarum: toward a potential application in wine

    OpenAIRE

    Capozzi, Vittorio; Russo, Pasquale; Ladero, Victor; Fernández, María; Fiocco, Daniela; Alvarez, Miguel A.; Grieco, Francesco; Spano, Giuseppe

    2012-01-01

    Biogenic amines in wine represent a toxicological risk for the health of the consumer, with several trade implications. In this study 26 strains of Lactobacillus plantarum were analysed for their ability to degrade biogenic amines commonly found during wine fermentation. Two strains of L. plantarum were selected in reason of their ability to degrade putrescine and tyramine. The degradation was assessed in vitro, both in presence of the biogenic amines and in presence of the specific chemical ...

  13. Control of biogenic amines in fermented sausages: role of starter cultures

    OpenAIRE

    MariluzLatorre-Moratalla; SaraBover-Cid

    2012-01-01

    Biogenic amines show biological activity and exert undesirable physiological effects when absorbed at high concentrations. Biogenic amines are mainly formed by microbial decarboxylation of amino acids and thus are usually present in a wide range of foods, fermented sausages being one of the major biogenic amine sources. The use of selected starter cultures is one of the best technological measures to control aminogenesis during meat fermentation. Although with variable effectiveness, several ...

  14. Control of Biogenic Amines in Fermented Sausages: Role of Starter Cultures

    OpenAIRE

    Latorre-Moratalla, M.L.; Bover-Cid, Sara; Veciana-Nogués, M.T.; Vidal-Carou, M.C.

    2012-01-01

    Biogenic amines show biological activity and exert undesirable physiological effects when absorbed at high concentrations. Biogenic amines are mainly formed by microbial decarboxylation of amino acids and thus are usually present in a wide range of foods, fermented sausages being one of the major biogenic amine sources. The use of selected starter cultures is one of the best technological measures to control aminogenesis during meat fermentation. Although with variable effectiveness, several ...

  15. Screening of biogenic amine production by lactic acid bacteria isolated from grape musts and wine

    OpenAIRE

    Moreno-Arribas, M. Victoria; Polo, María Carmen; Jorganes, Felisa; Muñoz, Rosario

    2003-01-01

    The potential to produce the biogenic amines tyramine, histamine and putrescine, was investigated for lactic acid bacteria (LAB) of various origin, including commercial malolactic starter cultures, type strains and 78 strains isolated from Spanish grape must and wine. The presence of biogenic amines in a decarboxylase synthetic broth was determined by reverse-phase high performance liquid chromatography (RP-HPLC). Tyramine was the main amine formed by the LAB strains investigated. ...

  16. Peptides that influence membrane topology

    Science.gov (United States)

    Wong, Gerard C. L.

    2014-03-01

    We examine the mechanism of a range of polypeptides that influence membrane topology, including antimicrobial peptides, cell penetrating peptides, viral fusion peptides, and apoptosis proteins, and show how a combination of geometry, coordination chemistry, and soft matter physics can be used to approach a unified understanding. We will also show how such peptides can impact biomedical problems such as auto-immune diseases (psoriasis, lupus), infectious diseases (viral and bacterial infections), and mitochondrial pathologies (under-regulated apoptosis leads to neurodegenerative diseases whereas over-regulated apoptosis leads to cancer.)

  17. NCAM Mimetic Peptides: An Update

    DEFF Research Database (Denmark)

    Berezin, Vladimir; Bock, Elisabeth

    2008-01-01

    sequences contain one or several NCAM homophilic binding sites involved in NCAM binding to itself, have been identified. By means of NMR titration analysis and molecular modeling a number of peptides derived from NCAM and targeting NCAM heterophilic ligands such as the fibroblast growth factor receptor...... and heparan sulfate proteoglycans (HSPG) have been identified. The FGL, dekaCAM, FRM/EncaminA, BCL, EncaminC and EncaminE peptides all target the FGF receptor whereas the heparin binding peptide HBP targets HSPG. Moreover, a number of NCAM binding peptides have been identified employing screening...

  18. Improving Peptide Applications Using Nanotechnology.

    Science.gov (United States)

    Narayanaswamy, Radhika; Wang, Tao; Torchilin, Vladimir P

    2016-01-01

    Peptides are being successfully used in various fields including therapy and drug delivery. With advancement in nanotechnology and targeted delivery carrier systems, suitable modification of peptides has enabled achievement of many desirable goals over-riding some of the major disadvantages associated with the delivery of peptides in vivo. Conjugation or physical encapsulation of peptides to various nanocarriers, such as liposomes, micelles and solid-lipid nanoparticles, has improved their in vivo performance multi-fold. The amenability of peptides to modification in chemistry and functionalization with suitable nanocarriers are very relevant aspects in their use and have led to the use of 'smart' nanoparticles with suitable linker chemistries that favor peptide targeting or release at the desired sites, minimizing off-target effects. This review focuses on how nanotechnology has been used to improve the number of peptide applications. The paper also focuses on the chemistry behind peptide conjugation to nanocarriers, the commonly employed linker chemistries and the several improvements that have already been achieved in the areas of peptide use with the help of nanotechnology. PMID:26279082

  19. Improving Peptide Applications Using Nanotechnology.

    Science.gov (United States)

    Narayanaswamy, Radhika; Wang, Tao; Torchilin, Vladimir P

    2016-01-01

    Peptides are being successfully used in various fields including therapy and drug delivery. With advancement in nanotechnology and targeted delivery carrier systems, suitable modification of peptides has enabled achievement of many desirable goals over-riding some of the major disadvantages associated with the delivery of peptides in vivo. Conjugation or physical encapsulation of peptides to various nanocarriers, such as liposomes, micelles and solid-lipid nanoparticles, has improved their in vivo performance multi-fold. The amenability of peptides to modification in chemistry and functionalization with suitable nanocarriers are very relevant aspects in their use and have led to the use of 'smart' nanoparticles with suitable linker chemistries that favor peptide targeting or release at the desired sites, minimizing off-target effects. This review focuses on how nanotechnology has been used to improve the number of peptide applications. The paper also focuses on the chemistry behind peptide conjugation to nanocarriers, the commonly employed linker chemistries and the several improvements that have already been achieved in the areas of peptide use with the help of nanotechnology.

  20. Biodiscovery of aluminum binding peptides

    Science.gov (United States)

    Adams, Bryn L.; Sarkes, Deborah A.; Finch, Amethist S.; Hurley, Margaret M.; Stratis-Cullum, Dimitra

    2013-05-01

    Cell surface peptide display systems are large and diverse libraries of peptides (7-15 amino acids) which are presented by a display scaffold hosted by a phage (virus), bacteria, or yeast cell. This allows the selfsustaining peptide libraries to be rapidly screened for high affinity binders to a given target of interest, and those binders quickly identified. Peptide display systems have traditionally been utilized in conjunction with organic-based targets, such as protein toxins or carbon nanotubes. However, this technology has been expanded for use with inorganic targets, such as metals, for biofabrication, hybrid material assembly and corrosion prevention. While most current peptide display systems employ viruses to host the display scaffold, we have recently shown that a bacterial host, Escherichia coli, displaying peptides in the ubiquitous, membrane protein scaffold eCPX can also provide specific peptide binders to an organic target. We have, for the first time, extended the use of this bacterial peptide display system for the biodiscovery of aluminum binding 15mer peptides. We will present the process of biopanning with macroscopic inorganic targets, binder enrichment, and binder isolation and discovery.

  1. LE MARCHE MONDIAL DU VIN ET LES NOUVELLES ORIENTATIONS DU MARKETING

    OpenAIRE

    Epuran Gheorghe

    2008-01-01

    Le marché mondial du vin est caractérisé par un développement sans précédent en ce qui concerne la production, mais aussi l’aire géographique qu’il comprend. La surproduction qui a tendance ŕ dégénérer en une crise est due premičrement ŕ l’apparition de nouveaux acteurs sur ce marché, c’est-ŕ-dire les pays du nouveau monde du vin: les Etats-Unis, le Chili, l’Afrique de Sud, l’Australie. Dans ce contexte, les grands producteurs traditionnels de l’Europe sont soumis ŕ une forte pression concurr...

  2. L’arme du chant collectif au cœur du conflit biafrais

    OpenAIRE

    Ugochukwu, Françoise

    2011-01-01

    Si des auteurs comme Basden, Leith-Ross et Nwando Achebe ont mentionné dans leurs écrits la puissance du chant dans la culture igbo, le rôle des chants nés du conflit biafrais et les raisons de leur diffusion sont des questions qui n’ont jamais encore été vraiment abordées - ce sera donc le but de cet article. Partant d’un disque de seize chants en igbo (6), anglais (8) et ijo (2) enregistrés pendant la guerre civile nigériane à 15km du front, juste avant la chute d’Umuahia, capitale provisoi...

  3. Les pathologies du Canal Péritonio-Vaginal

    OpenAIRE

    DJAZIRI, Sihem Fatima Zohra

    2014-01-01

    Les pathologies du canal péritonéo-vaginal, sont des affections congénitales dues à la persistance du canal au delà de la naissance. Elles regroupent les hernies inguinales et inguino-scrotales, les hydrocèles communicantes ou non, et les kystes du cordon spermatique. Notre travail est une étude rétrospective portant sur 309 malades opérés de pathologies du canal péritonio-vaginal dans le service de chirurgie infantile durant la période allant d’octobre 2011 jusqu'à octobre ...

  4. Stylistique(s) contrastive(s) du discours scientifique

    OpenAIRE

    Michel PETIT

    2012-01-01

    On postule que la stylistique a un rôle important à jouer dans l'étude du discours scientifique. Après avoir examiné les facteurs de diversité du discours scientifique et le statut de l'anglais scientifique, on s'intéresse à la question du style scientifique, notamment en termes d'expérience commune. On est enfin conduit, en étudiant les approches contrastives, à envisager une stylistique véritablement contrastive propre à rendre compte des spécificités du discours scientifique.

  5. Cloning and sequencing of Duck circovirus (DuCV).

    Science.gov (United States)

    Hattermann, K; Schmitt, C; Soike, D; Mankertz, A

    2003-12-01

    The genome of Duck circovirus (DuCV) is circular and 1996 nts in size. Two major open reading frames were identified, encoding the replicase (V1) and the capsid protein (C1). A stem-loop structure comprising the nonamer 5'-TATTATTAC, conserved in all circo-, nano- and geminiviruses, was found. Unique to DuCV, the region between the 3'-ends of the rep and cap gene contains four repeats of a 44-bp sequence. Phylogenetic analysis shows close relation of DuCV with Goose circovirus and suggests classification of DuCV as a new member of the genus Circovirus of the virus family Circoviridae. PMID:14648300

  6. Effect of radioprotective biogenic amines on peroxide oxidation of lipids in rat small intestine mucosa microsomes

    International Nuclear Information System (INIS)

    The radioprotective biogenic amines, dopamine, histamine, and serotonin inhibited lipid peroxidation in rat small intestine mucosal microsomes. Possible mechanisms of these inhibitory effects are discussed

  7. Mechanistic insights into the oxidative dehydrogenation of amines to nitriles in continuous flow

    DEFF Research Database (Denmark)

    Corker, Emily C.; Ruiz-Martínez, Javier; Riisager, Anders;

    2015-01-01

    The oxidative dehydrogenation of various aliphatic amines to their corresponding nitrile compounds using RuO2/Al2O3 catalysts in air was successfully applied to a continuous flow reaction. Conversions of amines (up to >99%) and yields of nitriles (up to 77%) varied depending on reaction conditions...... and the amine utilised. The presence of water was found to be important for the activity and stability of the RuO2/Al2O3 catalyst. The Hammett relationship and in situ infrared spectroscopy were applied to divulge details about the catalytic mechanism of the oxidative dehydrogenation of amines over Ru...

  8. Chemical and Molecular Descriptors for the Reactivity of Amines with CO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Anita S.; Kitchin, John R.

    2012-10-24

    Amine-based solvents are likely to play an important role in CO{sub 2} capture applications in the future, and the identification of amines with superior performance will facilitate their use in CO{sub 2} capture. While some improvements in performance will be achieved through process modifications, modifying the CO{sub 2} capture performance of an amine also implies in part an ability to modify the reactions between the amine and CO{sub 2} through development of new functionalized amines. We present a computational study of trends in the reactions between CO{sub 2} and functionalized amines with a focus on identifying molecular descriptors that determine trends in reactivity. We examine the formation of bicarbonate and carbamate species on three classes of functionalized amines: alkylamines, alkanolamines, and fluorinated alkylamines including primary, secondary and tertiary amines in each class. These functional groups span electron-withdrawing to donating behavior, hydrogen-bonding, extent of functionalization, and proximity effects of the functional groups. Electron withdrawing groups tend to destabilize CO{sub 2} reaction products, whereas electron-donating groups tend to stabilize CO{sub 2} reaction products. Hydrogen bonding stabilizes CO{sub 2} reaction products. Electronic structure descriptors based on electronegativity were found to describe trends in the bicarbonate formation energy. A chemical correlation was observed between the carbamate formation energy and the carbamic acid formation energy. The local softness on the reacting N in the amine was found to partially explain trends carbamic acid formation energy.

  9. Polyclonal Peptide Antisera.

    Science.gov (United States)

    Pihl, Tina H; Illigen, Kristin E; Houen, Gunnar

    2015-01-01

    Polyclonal antibodies are relatively easy to produce and may supplement monoclonal antibodies for some applications or even have some advantages. The choice of species for production of (peptide) antisera is based on practical considerations, including availability of immunogen (vaccine) and animals. Two major factors govern the production of antisera: the nature of adaptive immune responses, which take place over days/weeks and ethical guidelines for animal welfare. Here, simple procedures for immunization of mice, rabbits, sheep, goats, pigs, horses, and chickens are presented. PMID:26424267

  10. L’exercice autoritatif du blogueur et le genre éditorial du microblogging de Tumblr

    OpenAIRE

    Broudoux, Évelyne

    2014-01-01

    En tant qu’étape intermédiaire de recherche sur les relations qui unissent les concepts de « publication », de « réseaux sociaux » et de « Web de documents », cet article interroge le conditionnement du genre de la publication par des outils de communication en ligne. Quatre mois d’observation participante d’un outil de microblogging (Tumblr) en 2008 montrent les modifications du style et des comportements des auteurs. This article as an intermediary stage in a research—on the relations of...

  11. Les politiques straight du préservatif : VIH, pornographie et technologies du genre

    OpenAIRE

    Ribeiro, Kira

    2016-01-01

    Les productions pornographiques bisexuelles et « shemale » donnent à voir des configurations particulières de l’usage du préservatif qui informent tant sur les politiques du VIH que sur les politiques sexuelles au sein de cette industrie. Dans certaines de ces productions, les rapports entre hommes et femmes non-trans se font sans préservatif, tandis que dans la même scène et avec les mêmes performeurs, les rapports entre hommes ou impliquant une femme trans se font avec préservatif. À traver...

  12. 腐乳前酵过程中生物胺含量变化%Content of Biogenic Amines in Sufu During Pre-fermentation

    Institute of Scientific and Technical Information of China (English)

    王颖; 邱璠; 邢茜; 殷丽君

    2011-01-01

    采用丹磺酰氯柱前衍生、HPLC检测腐乳前酵过程中生物胺含量变化;同时,检测多肽、氨基态氮含量的变化。结果表明:在前酵过程中,多肽和氨基态氮的含量均呈增长趋势,生物胺总量的上升趋势与氨基态氮一致,总胺含量分别达到971.1mg/kg和782.0 mg/kg,但两种菌种发酵产物中,各种生物胺含量的变化趋势不尽相同,雅致放射毛霉发酵产物中,尸胺和酪胺含量分别达到460.1 mg/kg、318.5 mg/kg,两者共占生物胺总量的80.2%,其余6种生物胺含量均低于100 mg/kg;少孢根霉发酵产物中,尸胺含量为668.0 mg/kg,占生物胺总量的85.4%,其余7种生物胺含量均低于50 mg/kg。%The aim of this work was to describe the development of selected biogenic amines in sufu,fermented by Actinomucor elegans and Rhizopus oligosporus,respectively.Biogenic amines were determined by HPLC.Peptide and amino nitrogen were detected.The result indicated that the content of peptide and amino nitrogen increased in products fermented by Actinomucor elegans and Rhizopus oligosporus.The sum of eight biogenic amines corresponded with the content of amino nitrogen,and reached 971.1mg/kg and 782.0 mg/kg,respectively.But the dominant amines were different.Content of Cadaverine and Tyramine in product fermented by Actinomucor elegans reached 460.1 mg/kg and 318.5mg/kg,respectively,and the others were lower than 100 mg/kg.Content of Tyramine in product fermented by Rhizopus oligosporus reached 668.0 mg/kg,and the others were lower than 50 mg/kg.

  13. Physicochemical Changes of Antioxidant Peptides Hydrolyzed From Porcine Plasma Protein Subject to Free Hydroxyl Radical System

    Directory of Open Access Journals (Sweden)

    Hehong Yang

    2013-01-01

    Full Text Available Antioxidant peptides have attracted much attention for potential application as natural food ingredients but the fate of them, as well as oxidized proteins in foods during processing, is still poorly understood. Physicochemical changes in antioxidant peptides hydrolysated from porcine plasma protein were discussed in a free hydroxyl radical-mediated oxidation system. Porcine Plasma Protein Hydrolysates (PPH was prepared by hydrolyzing porcine plasma protein with Alcalase for 5 h at pH 8.0, 55°C. The content of carbonyl groups increased significantly at various degrees when PPH exposed to free radical-mediated oxidation for different time and different concentrations of H2O2, while total sulfhydryls, reactive sulfhydryls and free amines contents decreased. It was concluded that PPH played an antioxidant role in the radical-mediated oxidation system. This provides a potential way for antioxidation in food production.

  14. Secondary Amine Functional Disiloxanes as CO2 Sorbents

    Energy Technology Data Exchange (ETDEWEB)

    O' Brien, MJ; Farnum, RL; Perry, RJ; Genovese, SE

    2014-05-01

    A series of two different types of secondary amine functional disiloxanes were prepared and screened as CO2 capture solvents. The first group of materials contained RNHCH2CH2CH2 side chains where the R groups were C1-6 alkyls. When R was a primary alkyl group, these materials exhibited CO2 uptake values slightly in excess of theoretical. As the alkyl groups were changed to more sterically hindered secondary or tertiary alkyls, the uptake was less efficient. Heats of absorption values for these materials were generally in the range 2000-2200 kJ/kg of CO2, values significantly lower than those obtained for primary amine functional disiloxanes (2500-2700 kJ/kg of CO2). Also explored were a series of secondary amine functional disiloxanes with X-CH2CH2NH-CH2CH2CH2 - substituents. When X was an electron-donating group (RO-, R2N-, RO-CH2-) the CO2 uptake was also in excess of theoretical. Interestingly, these compounds were generally found to produce carbamate salts that were flowable, low-viscosity oils. Furthermore, the heat of absorption values determined for these materials were even lower. Most compounds gave values below 2000 kJ/kg of CO2. Overall the most promising results were obtained with a methoxyethylaminopropyl derivative, an ethoxyethylaminopropyl-containing material, and a dimethylaminoethylaminopropyl-based compound. These materials showed excellent CO2 uptake, had low heats of absorption, and produced carbamate salts that were flowable liquids even at room temperature.

  15. Lactoperoxidase-catalyzed activation of carcinogenic aromatic and heterocyclic amines.

    Science.gov (United States)

    Gorlewska-Roberts, Katarzyna M; Teitel, Candee H; Lay, Jackson O; Roberts, Dean W; Kadlubar, Fred F

    2004-12-01

    Lactoperoxidase, an enzyme secreted from the human mammary gland, plays a host defensive role through antimicrobial activity. It has been implicated in mutagenic and carcinogenic activation in the human mammary gland. The potential role of heterocyclic and aromatic amines in the etiology of breast cancer led us to examination of the lactoperoxidase-catalyzed activation of the most commonly studied arylamine carcinogens: 2-amino-1-methyl-6-phenylimidazo[4,5-b]-pyridine (PhIP), benzidine, 4-aminobiphenyl (ABP), 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx). In vitro activation was performed with lactoperoxidase (partially purified from bovine milk or human milk) in the presence of hydrogen peroxide and calf thymus DNA. Products formed during enzymatic activation were monitored by HPLC with ultraviolet and radiometric detection. Two of these products were characterized as hydrazo and azo derivatives by means of mass spectrometry. The DNA binding level of 3H- and 14C-radiolabeled amines after peroxidase-catalyzed activation was dependent on the hydrogen peroxide concentration, and the highest levels of carcinogen binding to DNA were observed at 100 microM H2O2. Carcinogen activation and the level of binding to DNA were in the order of benzidine > ABP > IQ > MeIQx > PhIP. One of the ABP adducts was identified, and the level at which it is formed was estimated to be six adducts/10(5) nucleotides. The susceptibility of aromatic and heterocyclic amines for lactoperoxidase-catalyzed activation and the binding levels of activated products to DNA suggest a potential role of lactoperoxidase-catalyzed activation of carcinogens in the etiology of breast cancer.

  16. Dissociation Behavior of a TEMPO-Active Ester Cross-Linker for Peptide Structure Analysis by Free Radical Initiated Peptide Sequencing (FRIPS) in Negative ESI-MS

    Science.gov (United States)

    Hage, Christoph; Ihling, Christian H.; Götze, Michael; Schäfer, Mathias; Sinz, Andrea

    2016-07-01

    We have synthesized a homobifunctional amine-reactive cross-linking reagent, containing a TEMPO (2,2,6,6-tetramethylpiperidine-1-oxy) and a benzyl group (Bz), termed TEMPO-Bz-linker, to derive three-dimensional structural information of proteins. The aim for designing this novel cross-linker was to facilitate the mass spectrometric analysis of cross-linked products by free radical initiated peptide sequencing (FRIPS). In an initial study, we had investigated the fragmentation behavior of TEMPO-Bz-derivatized peptides upon collision activation in (+)-electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) experiments. In addition to the homolytic NO-C bond cleavage FRIPS pathway delivering the desired odd-electron product ions, an alternative heterolytic NO-C bond cleavage, resulting in even-electron product ions mechanism was found to be relevant. The latter fragmentation route clearly depends on the protonation of the TEMPO-Bz-moiety itself, which motivated us to conduct (-)-ESI-MS, CID-MS/MS, and MS3 experiments of TEMPO-Bz-cross-linked peptides to further clarify the fragmentation behavior of TEMPO-Bz-peptide molecular ions. We show that the TEMPO-Bz-linker is highly beneficial for conducting FRIPS in negative ionization mode as the desired homolytic cleavage of the NO-C bond is the major fragmentation pathway. Based on characteristic fragments, the isomeric amino acids leucine and isoleucine could be discriminated. Interestingly, we observed pronounced amino acid side chain losses in cross-linked peptides if the cross-linked peptides contain a high number of acidic amino acids.

  17. SIRENA, une plateforme participative au service de la gouvernance du delta transfrontalier du fleuve Sénégal

    OpenAIRE

    Cormier-Salem, Marie-Christine; Toure, Labaly; Fabre, Mathilde; Bouaita, Yasmin; Ba Mamadou El Abass, Boubacar; Habert, Élisabeth

    2016-01-01

    Les zones humides littorales, écosystèmes aux services diversifiés et soumis à des pressions multiples, font l’objet de politiques de gestion contradictoires, balançant entre l’exploitation à des fins agricoles ou hydro-électriques et la conservation de la biodiversité. Tel est le cas du Delta du fleuve Sénégal, devenu Réserve de Biosphère en 2005, dans un contexte de mutations environnementales et socio-économiques rapides.Pour représenter les enjeux complexes et dynamiques du Delta du fleuv...

  18. Automatisation partielle du traitement de la littérature grise dans le service d'information scientifique du CERN

    CERN Document Server

    Deroche, C

    1998-01-01

    L'automatisation partielle du traitement de la litterature grise est un projet ineluctable dans un service d'information scientifique aussi important que celui du CERN. Ce rapport presente l'automatis ation du rapport annuel par la detection et l'importation automatique d'informations de publication pour les articles ecrits par les physiciens travaillant au CERN et par d'autres utilisant les outils du CERN. La recherche de ces articles est effectuee dans la base de donnees scientifiques INSPEC.

  19. In situ fabrication of cleavable peptide arrays on polydimethylsiloxane and applications for kinase activity assays

    International Nuclear Information System (INIS)

    Highlights: • A novel approach for in situ fabrication of cleavable peptide arrays on polydimethylsiloxane (PDMS). • The first report of peptide synthesis on PDMS. • Use of the PDMS peptide array for developing sensitive chip-based kinase activity bioassays. • The on-chip synthesized peptides can be cleaved for MS detection. - Abstract: Polydimethylsiloxane (PDMS) is widely used for microfabrication and bioanalysis; however, its surface functionalization is limited due to the lack of active functional groups and incompatibility with many solvents. We presented a novel approach for in situ fabrication of cleavable peptide arrays on polydimethylsiloxane (PDMS) viatert-butyloxycarbonyl (t-Boc)/trifluoroacetic acid (TFA) chemistry using gold nanoparticles (AuNPs) as the anchor and a disulfide/amine terminated hetero-polyethylene glycol as the cleavable linker. The method was fine tuned to use reagents compatible with the PDMS. Using 5-mer pentapeptide, Trp5, as a model, step-by-step covalent coupling during the reaction cycles was monitored by Attenuated total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), or atomic force microscopy (AFM), and further confirmed by mass spectrometry (MS) detection of the cleaved peptides. Using such a method, heptapeptides of the PKA substrate, LRRASLG (Kemptide), and its point mutated analogs were fabricated in an array format for comparative studies of cAMP-dependent protein kinase (PKA) activity. Based on on-chip detection, Kemptide sequence exhibited the highest phosphorylation activity, which was detected to a 1.5-time lesser extent for the point mutated sequence (LRRGSLG) containing the recognition motif (RRXS), and was nearly undetectable for another point mutated sequence (LRLASLG) that lacked the recognition motif. These results indicate that the reported fabrication method is able to yield highly specific peptide sequences on PDMS, leading to a highly motif

  20. In situ fabrication of cleavable peptide arrays on polydimethylsiloxane and applications for kinase activity assays

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Huang-Han, E-mail: z10008047@email.ncku.edu.tw; Hsiao, Yu-Chieh, E-mail: s10076221@hotmail.com; Li, Jie-Ren, E-mail: jierenli@mail.ncku.edu.tw; Chen, Shu-Hui, E-mail: shchen@mail.ncku.edu.tw

    2015-03-20

    Highlights: • A novel approach for in situ fabrication of cleavable peptide arrays on polydimethylsiloxane (PDMS). • The first report of peptide synthesis on PDMS. • Use of the PDMS peptide array for developing sensitive chip-based kinase activity bioassays. • The on-chip synthesized peptides can be cleaved for MS detection. - Abstract: Polydimethylsiloxane (PDMS) is widely used for microfabrication and bioanalysis; however, its surface functionalization is limited due to the lack of active functional groups and incompatibility with many solvents. We presented a novel approach for in situ fabrication of cleavable peptide arrays on polydimethylsiloxane (PDMS) viatert-butyloxycarbonyl (t-Boc)/trifluoroacetic acid (TFA) chemistry using gold nanoparticles (AuNPs) as the anchor and a disulfide/amine terminated hetero-polyethylene glycol as the cleavable linker. The method was fine tuned to use reagents compatible with the PDMS. Using 5-mer pentapeptide, Trp{sub 5}, as a model, step-by-step covalent coupling during the reaction cycles was monitored by Attenuated total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), or atomic force microscopy (AFM), and further confirmed by mass spectrometry (MS) detection of the cleaved peptides. Using such a method, heptapeptides of the PKA substrate, LRRASLG (Kemptide), and its point mutated analogs were fabricated in an array format for comparative studies of cAMP-dependent protein kinase (PKA) activity. Based on on-chip detection, Kemptide sequence exhibited the highest phosphorylation activity, which was detected to a 1.5-time lesser extent for the point mutated sequence (LRRGSLG) containing the recognition motif (RRXS), and was nearly undetectable for another point mutated sequence (LRLASLG) that lacked the recognition motif. These results indicate that the reported fabrication method is able to yield highly specific peptide sequences on PDMS, leading to a highly motif

  1. Processing method for drained water containing ethanol amine

    International Nuclear Information System (INIS)

    Drained water containing ethanol amine is processed with microorganisms such as hydrazine resistant denitrification bacteria in a biodegrading vessel (A) in the coexistence of nitrous ions and/or nitric ions under an anaerobic condition, and then it is processed with microorganisms such as nitrification bacteria in another biotic oxidation vessel (B) under an aerobic condition to generate the coexistent nitrate ion and/or nitric ion, and returned to the biodegrading vessel (A). Further, they are exposed to air or incorporated with an oxidant and optionally a copper compound such as copper sulfate as a catalyst is added in a step of removing hydrazine. (T.M.)

  2. The application experience of ethanol amine at KORI unit 4

    International Nuclear Information System (INIS)

    The secondary system water chemistry in the KORI PWR units has been well controlled by reducing the corrosion in the tubes of the steam generators; the pH agent has been changing from ammonia to ethanol amine (ETA) (1.8 to 2.0 ppm). For example, the iron concentration in the system was reduced by 40 to 70% compared with ammonia treatment. The paper presents the detailed information, such as ETA injection concentration and the variation of pH, concentration of organic acid products, and irons in the system, and a significant change on sludge after ETA application. (S. Ohno)

  3. Preparation of amine coated silver nanoparticles using triethylenetetramine

    Indian Academy of Sciences (India)

    L Ramajo; R Parra; M Reboredo; M Castro

    2009-01-01

    This article presents a simple method towards the preparation of functionalized silver nanoparticles in a continuous medium. Silver nanoparticles were obtained through AgNO3 chemical reduction in ethanol and triethylenetetramine was used to stabilize and functionalize the metal. The product was characterized with X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), UVvisible spectroscopy, thermal gravimetric analysis (TGA) and transmission electron microscopy (TEM). Monocrystalline silver particles with cubic structure and an average size of 20 nm were obtained. The results reveal that it is possible to synthesize Ag nanoparticles functionalized with amine groups and that particle size is influenced by the processing route.

  4. Carbon nanomaterial based electrochemical sensors for biogenic amines

    International Nuclear Information System (INIS)

    This review describes recent advances in the use of carbon nanomaterials for electroanalytical detection of biogenic amines (BAs). It starts with a short introduction into carbon nanomaterials such as carbon nanotubes, graphene, nanodiamonds, carbon nanofibers, fullerenes, and their composites. Next, electrochemical sensing schemes are discussed for various BAs including dopamine, serotonin, epinephrine, norepinephrine, tyramine, histamine and putrescine. Examples are then given for methods for simultaneous detection of various BAs. Finally, we discuss the current and future challenges of carbon nanomaterial-based electrochemical sensors for BAs. The review contains 175 references. (author)

  5. Biogenic amines and acute thermal stress in the rat

    Science.gov (United States)

    Williams, B. A.; Moberg, G. P.

    1975-01-01

    A study is summarized which demonstrates that depletion of the biogenic amines 5-hydroxytryptamine (5-HT) or norepinephrine (NE) alters the normal thermoregulatory responses to acute temperature stress. Specifically, NE depletion caused a significant depression in equilibrium rectal temperature at 22 C and a greater depression in rectal temperature than controls in response to cold (6 C) stress; NE depletion also resulted in a significantly higher rectal temperature response to acute heat (38 C) stress. Depletion of 5-HT had less severe effects. It remains unclear whether the primary site of action of these agents is central or peripheral.

  6. Pulmonary extraction of biogenic amines during septic shock

    International Nuclear Information System (INIS)

    The effect of live Escherichia coli on the pulmonary extraction of the biogenic amines 14C 5-hydroxytryptamine, (5-HT) and 3H-epinephrine was investigated. The labeled isotopes were injected into a central venous catheter and collected from an aortic catheter. One hundred per cent of the labeled epinephrine was recovered in the control and septic state. Only 32.8 +/- 3.6% SEM of the 5-hydroxytryptamine was recovered before sepsis and 42.5 +/- 4.9% SEM after sepsis. During sepsis, mean arterial pressure fell to 58 mm Hg from 121 mm Hg. Pulmonary shunt increased from .7 +/- .05 SEM to .33 +/- .09 SEM

  7. Aromatische Amine in der Gummiindustrie - Hautresorption, Belastung und Beanspruchung

    OpenAIRE

    Penkert, Sabine

    2012-01-01

    Aromatische Amine können Harnblasenkrebs verursachen. In der Gummiindustrie kann es zum Kontakt mit den potentiell kanzerogenen aromatischen Aminen Anilin und o-Toluidin kommen. Über die dermale Aufnahme dieser Substanzen unter realen Arbeitsbedingungen, insbesondere auch bei geschädigter Haut , ist bisher wenig bekannt. Auch die Effektivität von persönlichen Schutzmaßnahmen zur Reduktion der inneren Belastung ist kaum evaluiert. Das Ziel dieser Arbeit war es, zu untersuchen, inwieweit Beschä...

  8. Structural Influence on Excited State Dynamics in Simple Amines

    DEFF Research Database (Denmark)

    Klein, Liv Bærenholdt

    is femtosecond time-resolved photoelectron velocity map imaging (VMI), which is a newtechnique in the Copenhagen lab. The design, building and implementation of the VMI spectrometer has been a very substantial part of the thesis work. This techniques oers enhanced information content in the form of ecient...... and sensitive collection of photoelectron spectra. In particular, the angleresolved data available from the VMI approach provides highly detailed mechanistic insight about the relaxation pathways. One striking novel nding is that for tertiary amines, the critical factor driving the non-adiabatic dynamics...

  9. Electrophilic, Activation-Free Fluorogenic Reagent for Labeling Bioactive Amines.

    Science.gov (United States)

    Sintes, Miquel; De Moliner, Fabio; Caballero-Lima, David; Denning, David W; Read, Nick D; Kielland, Nicola; Vendrell, Marc; Lavilla, Rodolfo

    2016-06-15

    Herein we report the preparation of BODIPY mesoionic acid fluorides through a short sequence involving an isocyanide multicomponent reaction as the key synthetic step. These novel BODIPY acid fluorides are water-stable electrophilic reagents that can be used for the fluorescent derivatization of amine-containing biomolecules using mild and activation-free reaction conditions. As a proof of principle, we have labeled the antifungal natamycin and generated a novel fluorogenic probe for imaging a variety of human and plant fungal pathogens, with excellent selectivity over bacterial cells. PMID:27248580

  10. Analyse quantitative du réseau hydrographique du bassin versant du Slănic (Roumanie

    Directory of Open Access Journals (Sweden)

    Florina Grecu

    2007-12-01

    Full Text Available L'étude porte sur le bassin versant du Slănic, un affluent du Buzău. Ce bassin de taille relativement réduite (433 km2 est situé dans la région des Carpates et des Subcarpates de la Courbure, région très active sur le plan sismique. Du point de vue lithologique, le bassin du Slănic correspond à des flyschs paléogènes et à des molasses mio-pliocènes. Ces roches peu résistantes ont facilité une forte érosion du relief. Actuellement, les précipitations présentent souvent une forte intensité, et elles se produisent fréquemment après des périodes de sécheresse. Elles sont ainsi très agressives et déclenchent une érosion intense. L'analyse de quelques paramètres morphométriques [grand nombre de segments de cours d'eau d'ordres 1 (5230 et 2 (944 ; densité élevée des segments par rapport à la superficie du bassin (environ 15 segments / km2 ; longueur moyenne réduite des segments d'ordre 1 (260 m par rapport à ceux d'ordre 2 (530 m] s'accorde avec des temps de concentration des eaux courts et une forte énergie des écoulements. La vitesse de réaction aux précipitations des segments d'ordres inférieurs est en outre favorisée par la répartition spatiale des pluies et par les conditions lithologiques.The study is focused on the Slănic basin which has a small size (433 km2. The hydrographical basin of Slănic, situated in the Carpathian and Subcarpathian Curvature, a very active from neotectonical viewpoint area, is a part of the Buzău basin. From a petrographic point of view, the Slănic hydrographic basin belongs to the paleogen flysch deposits (within the Carpathian sector and to the mio-pliocen molasse (within the Subcarpathian area latter formed by predominantely friable rocks which have encouraged deep fragmentation of the relief. Actual rainfalls are often very intensive, and they frequently occur after dry seasons. The analysis of morphometric parameters shows many elementary segments of first

  11. Radiolabelled peptides for oncological diagnosis.

    NARCIS (Netherlands)

    Laverman, P.; Sosabowski, J.K.; Boerman, O.C.; Oyen, W.J.G.

    2012-01-01

    Radiolabelled receptor-binding peptides targeting receptors (over)expressed on tumour cells are widely under investigation for tumour diagnosis and therapy. The concept of using radiolabelled receptor-binding peptides to target receptor-expressing tissues in vivo has stimulated a large body of resea

  12. Urinary Peptides in Rett Syndrome.

    Science.gov (United States)

    Solaas, K. M.; Skjeldal, O.; Gardner, M. L. G.; Kase, B. F.; Reichelt, K. L.

    2002-01-01

    A study found a significantly higher level of peptides in the urine of 53 girls with Rett syndrome compared with controls. The elevation was similar to that in 35 girls with infantile autism. Levels of peptides were lower in girls with classic Rett syndrome than those with congenital Rett syndrome. (Contains references.) (Author/CR)

  13. Structural Characterization of Peptide Antibodies

    DEFF Research Database (Denmark)

    Chailyan, Anna; Marcatili, Paolo

    2015-01-01

    The role of proteins as very effective immunogens for the generation of antibodies is indisputable. Nevertheless, cases in which protein usage for antibody production is not feasible or convenient compelled the creation of a powerful alternative consisting of synthetic peptides. Synthetic peptide...

  14. Solid-phase peptide synthesis

    DEFF Research Database (Denmark)

    Jensen, Knud Jørgen

    2013-01-01

    This chapter provides an introduction to and overview of peptide chemistry with a focus on solid-phase peptide synthesis. The background, the most common reagents, and some mechanisms are presented. This chapter also points to the different chapters and puts them into perspective....

  15. A novel Indium-111-labeled gonadotropin-releasing hormone peptide for human prostate cancer imaging

    OpenAIRE

    Guo, Haixun; Gallazzi, Fabio; Sklar, Larry A.; Miao, Yubin

    2011-01-01

    The purpose of this study was to evaluate the tumor targeting and imaging properties of a novel 111In-labeled gonadotropin-releasing hormone (GnRH) peptide {1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-Ahx-(d-Lys6-GnRH1)} for human prostate cancer. The biodistribution and tumor imaging properties of 111In-DOTA-Ahx-(d-Lys6-GnRH1) were determined in DU145 human prostate cancer-xenografted nude mice. 111In-DOTA-Ahx-(d-Lys6-GnRH1) exhibited rapid tumor uptake (1.27 ± 0.40 %ID/g...

  16. L'aventure du grand collisionneur LHC du big bang au boson de Higgs

    CERN Document Server

    Denegri, Daniel

    2014-01-01

    Qu'est-ce que la physique des particules élémentaires, le LHC, et le boson de Higgs ? Ce livre présente de manière simple le monde des quarks, des leptons et de leurs interactions, gouvernées par des symétries fondamentales de la nature, ainsi que le lien entre ce monde de l'infiniment petit et celui de l'infiniment grand. Cette conjonction entre la physique des particules élémentaires et l'évolution de la matière dans les premiers instants de l Univers qui ont suivi le Big-Bang est un des plus beaux acquis de la science de ces cinquante dernières années. Après une description du cadre théorique, le modèle standard, et de son élaboration durant la deuxième moitié du XXe siècle, l'accent est mis sur ses grands succès expérimentaux, mais aussi sur ses faiblesses ou insuffisances telles que nous les percevons aujourd'hui. La passionnante histoire du grand collisionneur de hadrons du CERN, le LHC, le plus grand projet purement scientifique jamais réalisé, est présentée à la fois sous ses...

  17. Un Genevois coopte a la tete du Front islamique du salut

    CERN Multimedia

    Merckling, N

    2002-01-01

    "Le Front islamique du salut (FIS), a nomme a la tete de ce parti fondamentaliste un resident genevois, Mourad Dhani, chois parmi trois candidats. Ce physicien algerien est arrive a Geneve il y a 14 ans pour travailler au CERN (1 page).

  18. La reconstruction du sourcil par greffon composite du cuir chevelu: une astuce pour faciliter la technique

    Science.gov (United States)

    El Omari, Mounia; El Mazouz, Samir; Gharib, Noureddine; EL Abbassi, Abdallah

    2015-01-01

    Les sourcils jouent un rôle important dans l’équilibre esthétique du visage. Leur reconstruction ou ophriopoïése, après séquelle de brûlure fait partie intégrante du programme de réhabilitation de la face brûlée. Plusieurs techniques ont été décrites. Nous insistons ici sur l'intérêt d'une technique simple, à la portée de tous les chirurgiens, et dont la méthode et les résultats peuvent être améliorés par un dessin bien planifié des zones donneuse et receveuse: la greffe composite prélevée au niveau du cuir chevelu dessinée à l'aide d'un calque du sourcil controlatéral. PMID:26401195

  19. Sciences du comportement et de la communication: comportement du pharmacien en officine et comportement des usagers

    OpenAIRE

    Roosen, Marie

    1993-01-01

    Le comportement du pharmacien indique-t-il à l'usager ses rôles potentiels en éducation pour la santé dans un espace de santé ou traduit-il surtout un rôle d'opérateur commercial dans un espace de vente ?

  20. Prise en compte du spirituel et nouvelles représentations du soin

    OpenAIRE

    Chatel, Tanguy

    2006-01-01

    International audience To what extended comprehension of "care", the spiritual care in the secularized and public context of palliative care may lead us ? A quelles nouvelles représentations du soin nous convie une prise en compte de la souffrance spirituelle dans le contexte laïque et public des soins palliatifs ?

  1. LE MARCHE MONDIAL DU VIN ET LES NOUVELLES ORIENTATIONS DU MARKETING

    Directory of Open Access Journals (Sweden)

    Epuran Gheorghe

    2008-05-01

    Full Text Available Le marché mondial du vin est caractérisé par un développement sans précédent en ce qui concerne la production, mais aussi l’aire géographique qu’il comprend. La surproduction qui a tendance ŕ dégénérer en une crise est due premičrement ŕ l’apparition de nouveaux acteurs sur ce marché, c’est-ŕ-dire les pays du nouveau monde du vin: les Etats-Unis, le Chili, l’Afrique de Sud, l’Australie. Dans ce contexte, les grands producteurs traditionnels de l’Europe sont soumis ŕ une forte pression concurrentielle aussi bien sur leurs marchés qu’ŕ l’extérieur. Męme si en matičre d’image et de notoriété les vins de ces pays sont restés encore trčs connus, les pays du nouveau monde des vins ont réussi, par un marketing «ŕ l’américain», ŕ pénétrer en force sur plusieurs marchés, inclusivement sur les pays qui étaient pas traditionnellement consommateurs de vins (la Russie, la Chine, etc..

  2. Technological factors affecting biogenic amine content in foods: a review

    Directory of Open Access Journals (Sweden)

    Fausto Gardini

    2016-08-01

    Full Text Available Biogenic amines (BAs are molecules which can be present in foods and, due to their toxicity, can cause adverse effects on the consumers. BAs are generally produced by microbial decarboxylation of amino acids in food products. The most significant BAs occurring in foods are histamine, tyramine, putrescine, cadaverine, tryptamine, 2-phenylethylamine, spermine, spermidine and agmatine. The importance of preventing the excessive accumulation of BAs in food is related to their impact on human health and food quality. Quality criteria in connection with the presence of BAs in food and food products are necessary from a toxicological point of view. This is particularly important in fermented foods in which the massive microbial proliferation required for obtaining specific products is often relater with BA accumulation. In this review, up-to-date information and recent discoveries about technological factors affecting biogenic amine content in foods are reviewed. Specifically, BA forming-microorganism and decarboxylation activity, genetic and metabolic organization of decarboxylases, risk associated to BAs (histamine, tyramine toxicity and other BAs, environmental factors influencing BA formation (temperature, salt concentration, pH. In addition, the technological factors for controlling BA production (use of starter culture, technological additives, effects of packaging, other non-thermal treatments, metabolising BA by microorganisms, effects of pressure treatments on BA formation and antimicrobial substances are addressed.

  3. Analysis of irradiated biogenic amines by computational chemistry and spectroscopy

    International Nuclear Information System (INIS)

    Biogenic Amines (B A) are nitrogenous compounds able to cause food poisoning. In this work, we studied the tyramine, one of the most common BA present in foods by combining experimental measured IR (Infrared) and GC/MS (Gas Chromatograph / Mass Spectrometry) spectra and computational quantum chemistry. Density Functional Theory (DFT) and the Deformed Atoms in Molecules (DMA) method was used to compute the partition the electronic densities in a chemically-intuitive way and electrostatic potentials of molecule to identify the acid and basic sites. Trading pattern was irradiated using a Cs 137 radiator, and each sample was identified by IR and GC/MS. Calculated and experimental IR spectra were compared. We observed that ionizing gamma irradiation was very effective in decreasing the population of standard amine, resulting in fragments that could be rationalized through the quantum chemistry calculations. In particular, we could locate the acid and basic sites of both molecules and identify possible sites of structural weaknesses, which allowed to propose mechanistic schemes for the breaking of chemical bonds by the irradiation. Moreover, from this work we hope it will be also possible to properly choose the dose of gamma irradiation which should be provided to eliminate each type of contamination. (author)

  4. Analysis of biogenic amines using corona discharge ion mobility spectrometry.

    Science.gov (United States)

    Hashemian, Z; Mardihallaj, A; Khayamian, T

    2010-05-15

    A new method based on corona discharge ion mobility spectrometry (CD-IMS) was developed for the analysis of biogenic amines including spermidine, spermine, putrescine, and cadaverine. The ion mobility spectra of the compounds were obtained with and without n-Nonylamine used as the reagent gas. The high proton affinity of n-Nonylamine prevented ion formation from compounds with a proton affinity lower than that of n-Nonylamine and, therefore, enhanced its selectivity. It was also realized that the ion mobility spectrum of n-Nonylamine varied with its concentration. A sample injection port of a gas chromatograph was modified and used as the sample introduction system into the CD-IMS. The detection limits, dynamic ranges, and analytical parameters of the compounds with and without using the reagent gas were obtained. The detection limits and dynamic ranges of the compounds were about 2ng and 2 orders of magnitude, respectively. The wide dynamic range of CD-IMS originates from the high current of the corona discharge. The results revealed the high capability of the CD-IMS for the analysis of biogenic amines. PMID:20298897

  5. Factors influencing biogenic amines accumulation in dairy products.

    Science.gov (United States)

    Linares, Daniel M; Del Río, Beatriz; Ladero, Victor; Martínez, Noelia; Fernández, María; Martín, María Cruz; Alvarez, Miguel A

    2012-01-01

    Fermented foods are among the food products more often complained of having caused episodes of biogenic amines (BA) poisoning. Concerning milk-based fermented foods, cheese is the main product likely to contain potentially harmful levels of BA, specially tyramine, histamine, and putrescine. Prompted by the increasing awareness of the risks related to dietary uptake of high biogenic amine loads, in this review we report all those elaboration and processing technological aspects affecting BA biosynthesis and accumulation in dairy foods. Improved knowledge of the factors involved in the synthesis and accumulation of BA should lead to a reduction in their incidence in milk products. Synthesis of BA is possible only when three conditions converge: (i) availability of the substrate amino acids; (ii) presence of microorganisms with the appropriate catabolic pathway activated; and (iii) environmental conditions favorable to the decarboxylation activity. These conditions depend on several factors such as milk treatment (pasteurization), use of starter cultures, NaCl concentration, time, and temperature of ripening and preservation, pH, temperature, or post-ripening technological processes, which will be discussed in this chapter. PMID:22783233

  6. BIODEGRADATION OF AROMATIC AMINE COMPOUNDS USING MOVING BED BIOFILM REACTORS

    Directory of Open Access Journals (Sweden)

    M. Delnavaz ، B. Ayati ، H. Ganjidoust

    2008-10-01

    Full Text Available Three moving bed biofilm reactors were used to treat synthesized wastewater of aromatic amine compounds including aniline, para-diaminobenzene and para-aminophenol that are found in many industrial wastewaters. The reactors with cylindrical shape had an internal diameter and an effective depth of 10 and 60 cm, respectively. The reactors were filled with light expanded clay aggregate as carriers and operated in an aerobic batch and continuous conditions. Evaluation of the reactors' efficiency was done at different retention time of 8, 24, 48 and 72 h with an influent COD from 100 to 3500 mg/L (filling ratio of 50%. The maximum obtained removal efficiencies were 90% (influent COD=2000 mg/L, 87% (influent COD=1000 mg/L and 75% (influent COD=750 mg/L for aniline, para-diaminobenzene and para-aminophenol, respectively. In the study of decrease in filling ratio from 50 to 30 percent, 6% decrease for both para-diaminobenzene and para-aminophenol and 7% increase for aniline degradation were obtained. The removal efficiency was decreased to about 10% after 15 days of continuous loading for each of the above three substrates. In the shock loading test, initially the COD removal rate was decreased in all reactors, but after about 10 days, it has been approached to the previous values. Finally, biodegradability of aromatic amines has been proved by nuclear magnetic resonance system.

  7. Mild Metal-Free Hydrosilylation of Secondary Amides to Amines.

    Science.gov (United States)

    Huang, Pei-Qiang; Lang, Qi-Wei; Wang, Yan-Rong

    2016-05-20

    The combination of amide activation by Tf2O with B(C6F5)3-catalyzed hydrosilylation with TMDS constitutes a method for the one-pot reduction of secondary amides to amines under mild conditions. The method displays a broad applicability for the reduction of many types of substrates, and shows good compatibility and excellent chemoselectivity for many sensitive functional groups. Reductions of a multifunctionalized α,β-unsaturated amide obtained from another synthetic methodology, and a C-H functionalization product produced the corresponding amines in good to excellent yield. Chemoselective reduction of enantiomeric pure (ee >99%) tetrahydro-5-oxo-2-furaneamides yielded 5-(aminomethyl)dihydrofuran-2(3H)-ones in a racemization-free manner. The latter were converted in one pot to N-protected 5-hydroxypiperidin-2-ones, which are building blocks for the synthesis of many natural products. Further elaboration of an intermediate led to a concise four-step synthesis of (-)-epi-pseudoconhydrine. PMID:27100232

  8. Analysis of Some Biogenic Amines by Micellar Liquid Chromatography

    Directory of Open Access Journals (Sweden)

    Irena Malinowska

    2012-01-01

    Full Text Available Micellar liquid chromatography (MLC with the use of high performance liquid chromatography (HPLC was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine. In this paper, an influence of surfactant’s concentration and pH of the micellar mobile phase on the retention of the tested substances was examined. To determine the influence of surfactant’s concentration on the retention of the tested amines, buffered solutions (at pH 7.4 of ionic surfactant—sodium dodecyl sulfate SDS (at different concentrations with acetonitrile as an organic modifier (0.8/0.2 v/v were used as the micellar mobile phases. To determine the influence of pH of the micellar mobile phase on the retention, mobile phases contained buffered solutions (at different pH values of sodium dodecyl sulfate SDS (at 0.1 M with acetonitrile (0.8/0.2 v/v. The inverse of value of retention factor (1/ versus concentration of micelles ( relationships were examined. Other physicochemical parameters of solutes such as an association constant analyte—micelle (ma—and partition coefficient of analyte between stationary phase and water (hydrophobicity descriptor (swΦ were determined by the use of Foley’s equation.

  9. Amine-oxide hybrid materials for acid gas separations

    KAUST Repository

    Bollini, Praveen

    2011-01-01

    Organic-inorganic hybrid materials based on porous silica materials functionalized with amine-containing organic species are emerging as an important class of materials for the adsorptive separation of acid gases from dilute gas streams. In particular, these materials are being extensively studied for the adsorption of CO 2 from simulated flue gas streams, with an eye towards utilizing these materials as part of a post-combustion carbon capture process at large flue gas producing installations, such as coal-fired electricity-generating power plants. In this Application Article, the utilization of amine-modified organic-inorganic hybrid materials is discussed, focusing on important attributes of the materials, such as (i) CO 2 adsorption capacities, (ii) adsorption and desorption kinetics, and (iii) material stability, that will determine if these materials may one day be useful adsorbents in practical CO 2 capture applications. Specific research needs and limitations associated with the current body of work are identified. © 2011 The Royal Society of Chemistry.

  10. Rôle des acides aminés dans la production d'amines biogènes chez Oenococcus oeni

    OpenAIRE

    Dandach, Said,

    2013-01-01

    Dans le vin, les amines biogènes sont essentiellement d'origine microbienne, et sont produitesnotamment par les bactéries lactiques Oenococcus oeni, principal agent responsable de lafermentation malolactique, possède de nombreuses auxotrophies vis-à-vis des acides aminés.Aucune étude n'a été menée sur la relation entre l’auxotrophie vis-à-vis d’un acide aminé et leniveau de l’amine correspondante. 80 souches de Oenococcus oeni ont été isolées de vinsrouges. Leurs auxotrophies vis-à-vis d’acid...

  11. Application of ultraviolet, ozone, and advanced oxidation treatments to washwaters to destroy nitrosamines, nitramines, amines, and aldehydes formed during amine-based carbon capture.

    Science.gov (United States)

    Shah, Amisha D; Dai, Ning; Mitch, William A

    2013-03-19

    Although amine-based CO(2) absorption is a leading contender for full-scale postcombustion CO(2) capture at power plants, concerns have been raised about the potential release of carcinogenic N-nitrosamines and N-nitramines formed by reaction of exhaust gas NO(x) with the amines. Experiments with a laboratory-scale pilot unit suggested that washwater units meant to scrub contaminants from absorber unit exhaust could potentially serve as a source of N-nitrosamines via reactions of residual NO(x) with amines accumulating in the washwater. Dosage requirements for the continuous treatment of the washwater recycle line with ultraviolet (UV) light for destruction of N-nitrosamines and N-nitramines, and with ozone or hydroxyl radical-based advanced oxidation processes (AOPs) for destruction of amines and aldehydes, were evaluated. Although capture synergies between UV and ozone treatments.

  12. Role of Du Channel in Treating Senile Dementia

    Institute of Scientific and Technical Information of China (English)

    Lu Fan

    2006-01-01

    @@ The author believes that senile dementia is very closely related to not only deficiency of the liver and kidney, but also Eight Extra Channels, Du Channel in particular. Nourishing the liver and kidney and clearing Du Channel in the treatment can greatly enhance the therapeutic effect.

  13. Cyber Dhaba: un kiosque internet en Inde du Nord

    CERN Multimedia

    2002-01-01

    Il y a deux ans, l'association Orient Yourself installait un PC connecté sous un banyan sacré, dans un village agricole du Rajasthan (Inde du Nord). Claire Dixsaut dirige ELSE, elle est membre fondateur de l'association Orient Yourself.

  14. Du Yuzhou Art Exhibition Will Open in the United Nations

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    From June 5th to 15th 2007,the headquarters of the United Nations will enjoy a show time of Du Yuzhou works,where works of photograph and painting will be exhibited. Mr.Yuzhou Du,President of the China National

  15. Le pari du numérique

    Directory of Open Access Journals (Sweden)

    Marie-Françoise Durand

    2011-01-01

    Full Text Available En lançant ce premier numéro du Ceriscope, le Centre de recherches et d’études internationales (CERI et l’Atelier de cartographie de Sciences Po ont conscience de relever un défi. Engagés dans l’analyse scientifique des sociétés du monde, le CERI, en tant que centre de recherche, et l’Atelier de cartographie, en sa qualité de concepteurs de cartes, entendent faire accéder un grand nombre de lecteurs à leurs productions en utilisant la gratuité d’Internet. L’avantage d’une publication en ligne par rapport au livre papier ne se résume évidemment pas à la gratuité. Elle permet en effet un usage plus souple de la lecture en autorisant, grâce à l’indexation par mots clés, une recherche à l’intérieur des textes. Elle offre ensuite une rapidité de mise à jour des connaissances qui, compte tenu de l’évolution de l’économie et des sociétés, sont vite touchées par l’obsolescence. Les contributions mises en ligne seront ainsi actualisées et de nouvelles publications pourront les développer et les enrichir au fil du temps. La publication en ligne permet enfin les réactions des lecteurs qui deviennent ainsi des « acteurs » de la publication.

  16. Modelisation par elements finis du muscle strie

    Science.gov (United States)

    Leonard, Mathieu

    Ce present projet de recherche a permis. de creer un modele par elements finis du muscle strie humain dans le but d'etudier les mecanismes engendrant les lesions musculaires traumatiques. Ce modele constitue une plate-forme numerique capable de discerner l'influence des proprietes mecaniques des fascias et de la cellule musculaire sur le comportement dynamique du muscle lors d'une contraction excentrique, notamment le module de Young et le module de cisaillement de la couche de tissu conjonctif, l'orientation des fibres de collagene de cette membrane et le coefficient de poisson du muscle. La caracterisation experimentale in vitro de ces parametres pour des vitesses de deformation elevees a partir de muscles stries humains actifs est essentielle pour l'etude de lesions musculaires traumatiques. Le modele numerique developpe est capable de modeliser la contraction musculaire comme une transition de phase de la cellule musculaire par un changement de raideur et de volume a l'aide des lois de comportement de materiau predefinies dans le logiciel LS-DYNA (v971, Livermore Software Technology Corporation, Livermore, CA, USA). Le present projet de recherche introduit donc un phenomene physiologique qui pourrait expliquer des blessures musculaires courantes (crampes, courbatures, claquages, etc.), mais aussi des maladies ou desordres touchant le tissu conjonctif comme les collagenoses et la dystrophie musculaire. La predominance de blessures musculaires lors de contractions excentriques est egalement exposee. Le modele developpe dans ce projet de recherche met ainsi a l'avant-scene le concept de transition de phase ouvrant la porte au developpement de nouvelles technologies pour l'activation musculaire chez les personnes atteintes de paraplegie ou de muscles artificiels compacts pour l'elaboration de protheses ou d'exosquelettes. Mots-cles Muscle strie, lesion musculaire, fascia, contraction excentrique, modele par elements finis, transition de phase

  17. CZE study on adsorption processes of aliphatic and aromatic amines on PMMA chip.

    Science.gov (United States)

    Masár, Marián; Kruk, Pavol; Luc, Milan; Bodor, Róbert; Danč, Ladislav; Troška, Peter

    2013-02-01

    Adsorption processes on a PMMA chip linked with CZE separations of a group of 13 aliphatic and aromatic mono- and di-amines were studied. Due to the lack of chromophores within aliphatic amines, contact conductivity detection implemented directly onto the chip was used for monitoring of cationic CZE separations. To prevent an adsorption of studied amines to the chip channels, the surface of PMMA chip was modified by dynamic coating. Different surface modifiers, such as aliphatic oligoamines (diethylenetriamine and triethylenetetramine), were added to the BGE solutions filling the chip channels. The effect of various concentrations of surface modifiers on peak profiles and separation parameters of amines was monitored. Of these, mainly, aliphatic di-amines and aromatic mono-amines adversely affected the CZE resolution of a whole group of analytes by their strong adsorption to the chip channels. A propionate BGE with pH 3.2 containing 100 μM triethylenetetramine and 25 mM 18-crown-6-ether was found suitable for CZE resolution of 12 from a total of 13 amines studied. Simple dynamic modification of the surface of PMMA chip enabled fast (analysis time lasted 9 min), sensitive (sub-μM LODs reached) and reproducible (1-3% RSD of the peak areas) CZE analysis of the aliphatic and aromatic amines.

  18. Solid-phase Synthesis of a Novel Kind of Hydroxylated Heterocyclic Ketene Aminals

    Institute of Scientific and Technical Information of China (English)

    Tao PENG; Chu Yi YU; Zhi Tang HUANG

    2006-01-01

    An efficient solid-phase synthesis method for novel heterocyclic ketene aminals containing a hydroxyl group has been developed. The loading of the substrate on the resin through the hydroxyl group and the protection of the amine by the Schiff base were the key steps in the synthesis.

  19. Amination of Aryl Halides and Esters Using Intensified Continuous Flow Processing

    OpenAIRE

    Thomas M. Kohl; Christian H. Hornung; John Tsanaktsidis

    2015-01-01

    Significant process intensification of the amination reactions of aryl halides and esters has been demonstrated using continuous flow processing. Using this technology traditionally difficult amination reactions have been performed safely at elevated temperatures. These reactions were successfully conducted on laboratory scale coil reactor modules with 1 mm internal diameter (ID) and on a preparatory scale tubular reactor with 6 mm ID containing static mixers.

  20. Plasma semicarbazide-sensitive amine oxidase is elevated in patients with congestive heart failure

    NARCIS (Netherlands)

    Boomsma, F; vanVeldhuisen, DJ; deKam, PJ; ManintVeld, AJ; Mosterd, A; Lie, KI; Schalekamp, MADH

    1997-01-01

    Objective: Semicarbazide-sensitive amine oxidase (SSAO) is present in various mammalian tissues, especially in vascular smooth muscle cells, but also in plasma. The enzyme has been suggested to play a role in vascular endothelial damage through conversion of amines into cytotoxic aldehydes, ammonia

  1. Facile one-pot synthesis of unsymmetrical ureas, carbamates, and thiocarbamates from Cbz-protected amines.

    Science.gov (United States)

    Kim, Hee-Kwon; Lee, Anna

    2016-07-26

    A novel one-pot synthesis of unsymmetrical ureas, carbamates and thiocarbamates from Cbz-protected amines has been developed. In the presence of 2-chloropyridine and trifluoromethanesulfonyl anhydride, isocyanates are generated in situ, which facilitate rapid reaction with amines, alcohols, and thiols to afford the corresponding ureas, carbamates and thiocarbamates in high yields. PMID:27406041

  2. Analysis of primary aromatic amines (PAA) in black nylon kitchenware 2014

    DEFF Research Database (Denmark)

    Trier, Xenia; Granby, Kit

    shall be accompanied by appropriate documentation, including analytical results showing that it meets the requirements concerning the release of primary aromatic amines. 25 samples of black nylon kitchenware each of three articles were tested for migration of primary aromatic amines (PAA), using 3...

  3. Genome Sequence Analysis of the Biogenic Amine-Degrading Strain Lactobacillus casei 5b

    OpenAIRE

    Ladero Losada, Víctor Manuel; Herrero, Ana; Martínez Álvarez, Noelia; Río Lagar, Beatriz del; Linares, Daniel M.; Fernández García, María; Martín, M. Cruz; Álvarez González, Miguel Ángel

    2014-01-01

    We here report a 3.02-Mbp annotated draft assembly of the Lactobacillus casei 5b genome. The sequence of this biogenic amine-degrading dairy isolate may help identify the mechanisms involved in the catabolism of biogenic amines and perhaps shed light on ways to reduce the presence of these toxic compounds in food.

  4. Differential behavioral responses of two plant-parasitic nematodes to biogenic amines

    Science.gov (United States)

    Hatching and infective juvenile (J2) behavior in two species of plant-parasitic nematodes, Heterodera glycines and Meloidogyne incognita, were affected by in vitro treatment with the biogenic amines dopamine, octopamine, and serotonin. While the overall responses of each species to amine exposures w...

  5. Concentration of Biogenic Amines in ‘Pinot Noir’ Wines Produced in Croatia

    Directory of Open Access Journals (Sweden)

    Ana Jeromel

    2014-02-01

    Full Text Available The origins of biogenic amines are sound grapes, alcoholic fermentations, malolactic fermentation and microbial activities during wine storage. These biologically produced amines are essential at low concentrations for optimal metabolic and physiological functions in animals, plants and micro-organisms. During alcoholic fermentation the degree of maceration is the first factor that affects the extraction of compounds present in the grape skin, among them aminoacids, precursors of biogenic amines. The aim of the present work was to study the changes of the concentration of biogenic amines in wines made from Vitis vinifera ‘Pinot noir’ from Plešivica (vintage 2009 produced with classical maceration, cold maceration and use of sur lie method. Biogenic amines were quantified using a reversed-phase high performance liquid chromatography (HPLC with fluorescence detection after pre-column derivatization with o-phthalaldehyde (OPA. In ‘Pinot noir’ wines tested, histamine was the most abundant biogenic amine followed by tryptamine and 2-Phenylethylamine. Total amount of biogenic amines ranged from 8.72 mg/L in wines made with classical maceration up to 9.34 mg/L in sur lie wines. In summary, from the results obtained in this study, it can be concluded that sur lie technology can influence the formation of biogenic acids since the release of amino acids is probably more pronounced in wines aged with lees and stirred weekly. No significant differences were found in the concentration of biogenic amines in relation to the used maceration process.

  6. Long-chain amine-templated synthesis of gallium sulfide and gallium selenide nanotubes

    Science.gov (United States)

    Seral-Ascaso, A.; Metel, S.; Pokle, A.; Backes, C.; Zhang, C. J.; Nerl, H. C.; Rode, K.; Berner, N. C.; Downing, C.; McEvoy, N.; Muñoz, E.; Harvey, A.; Gholamvand, Z.; Duesberg, G. S.; Coleman, J. N.; Nicolosi, V.

    2016-06-01

    We describe the soft chemistry synthesis of amine-templated gallium chalcogenide nanotubes through the reaction of gallium(iii) acetylacetonate and the chalcogen (sulfur, selenium) using a mixture of long-chain amines (hexadecylamine and dodecylamine) as a solvent. Beyond their role as solvent, the amines also act as a template, directing the growth of discrete units with a one-dimensional multilayer tubular nanostructure. These new materials, which broaden the family of amine-stabilized gallium chalcogenides, can be tentatively classified as direct large band gap semiconductors. Their preliminary performance as active material for electrodes in lithium ion batteries has also been tested, demonstrating great potential in energy storage field even without optimization.We describe the soft chemistry synthesis of amine-templated gallium chalcogenide nanotubes through the reaction of gallium(iii) acetylacetonate and the chalcogen (sulfur, selenium) using a mixture of long-chain amines (hexadecylamine and dodecylamine) as a solvent. Beyond their role as solvent, the amines also act as a template, directing the growth of discrete units with a one-dimensional multilayer tubular nanostructure. These new materials, which broaden the family of amine-stabilized gallium chalcogenides, can be tentatively classified as direct large band gap semiconductors. Their preliminary performance as active material for electrodes in lithium ion batteries has also been tested, demonstrating great potential in energy storage field even without optimization. Electronic supplementary information (ESI) available. See DOI: 10.1039/c6nr01663d

  7. Benzoyl peroxide (BPO)-promoted oxidative trifluoromethylation of tertiary amines with trimethyl(trifluoromethyl)silane.

    Science.gov (United States)

    Chu, Lingling; Qing, Feng-Ling

    2010-09-14

    The benzoyl peroxide (BPO)-promoted oxidative functionalization of tertiary amines under transition-metal-free reaction conditions was developed. Various 1-trifluoromethylated tetrahydroisoquinoline derivatives were prepared by employing this method. It constitutes the first example of direct trifluoromethylation of tertiary amines.

  8. 40 CFR 721.9620 - Aromatic sulfonic acid compound with amine.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Aromatic sulfonic acid compound with... Specific Chemical Substances § 721.9620 Aromatic sulfonic acid compound with amine. (a) Chemical substance... aromatic sulfonic acid compound with amine (PMN P-93-832) is subject to reporting under this section...

  9. Emissions, sinks and gas to particle conversion of amines and ammonia

    Science.gov (United States)

    Lee, S.

    2015-12-01

    Nitrogen-containing base compounds, amines and ammonia, play important roles in formation of secondary aerosols in the atmosphere, but their sources, sinks and atmospheric transformation processes are not well understood. Also, there are very limited analytical methods that are capable of measuring pptv or sub-pptv level of amines and ammonia. We have developed a chemical ionization mass spectrometer (CIMS) that can detect amines and ammonia at the pptv and sub-pptv level with a 1 min of integration time. Here, we report ambient measurements of amines and ammonia made in a moderately polluted continental site (Kent, Ohio) and in a rural Southeastern U.S. forest (Centreville, Alabama). Our finding indicate that there are much more abundant gas phase amines (C1-C6) and ammonia in the polluted site than in the rural forest, highlighting the importance of constraining anthropogenic emission sources of amines. At both locations, concentrations of these base compounds show clear temperature dependence, indicating strong gas-to-particle conversion processes. Compared to ammonia, amines can partition into aerosol phases even more effectively due to lower saturation vapor pressures. Measurements in the clean rural forest show that transported biomass burning plumes are the major source of amines. These nitrogen-containing compounds effectively undergo wet deposition in the atmosphere due to high solubilities.

  10. 21 CFR 173.70 - Chloromethylated aminated styrene-divinylbenzene resin.

    Science.gov (United States)

    2010-04-01

    ... resin. 173.70 Section 173.70 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... Chloromethylated aminated styrene-divinylbenzene resin. Chloromethylated aminated styrene-divinylbenzene copolymer... resin dispersion. (c) The additive is used as a decolorizing and clarification agent for treatment...

  11. N-heterocyclic carbene copper(I) catalysed N-methylation of amines using CO2

    KAUST Repository

    Santoro, Orlando

    2015-09-30

    The N-methylation of amines using CO2 and PhSiH3 as source of CH3 was efficiently performed using a N-heterocyclic carbene copper(I) complex. The methodology was found compatible with aromatic and aliphatic primary and secondary amines. Synthetic and computational studies have been carried out to support the proposed reaction mechanism for this transformation.

  12. The Petasis Reaction: Microscale Synthesis of a Tertiary Amine Antifungal Analog

    Science.gov (United States)

    Koroluk, Katherine J.; Jackson, Derek A.; Dicks, Andrew P.

    2012-01-01

    Students prepare a tertiary amine antifungal analog in an upper-level undergraduate organic laboratory. A microscale Petasis reaction is performed to generate a liquid compound readily characterized via IR and proton NMR spectroscopy. The biological relevance of the product is highlighted, with the tertiary amine scaffold being an important…

  13. INTERACTIONS BETWEEN KETONES AND AMINES IN PHOTOINITIATING SYSTEMS USED FOR RADICAL POLYMERIZATION

    Institute of Scientific and Technical Information of China (English)

    WU Shikang; FOUASSIER ,J. P.

    1990-01-01

    Time resolved laser spectroscopy and GC/MS were used to investigate the primary processes and the side reactions occurring in benzophenone and chlorothioxanthone/amine systems used as photoinitiators of radical polymerization. The discrepancy observed between the experimental data and the expected behaviour is accounted for by detrimental chemicalreaction involving the ketone triplet state, the ketyl radical and the amine - derived radical.

  14. Concentration of Biogenic Amines in ‘Pinot Noir’ Wines Produced in Croatia

    Directory of Open Access Journals (Sweden)

    Ana Jeromel

    2012-03-01

    Full Text Available The origins of biogenic amines are sound grapes, alcoholic fermentations, malolactic fermentation and microbial activities during wine storage. These biologically produced amines are essential at low concentrations for optimal metabolic and physiological functions in animals, plants and micro-organisms. During alcoholic fermentation the degree of maceration is the first factor that affects the extraction of compounds present in the grape skin, among them aminoacids, precursors of biogenic amines. The aim of the present work was to study the changes of the concentration of biogenic amines in wines made from Vitis vinifera ‘Pinot noir’ from Plešivica (vintage 2009 produced with classical maceration, cold maceration and use of sur lie method. Biogenic amines were quantified using a reversed-phase high performance liquid chromatography (HPLC with fluorescence detection after pre-column derivatization with o-phthalaldehyde (OPA. In ‘Pinot noir’ wines tested, histamine was the most abundant biogenic amine followed by tryptamine and 2-Phenylethylamine. Total amount of biogenic amines ranged from 8.72 mg/L in wines made with classical maceration up to 9.34 mg/L in sur lie wines. In summary, from the results obtained in this study, it can be concluded that sur lie technology can influence the formation of biogenic acids since the release of amino acids is probably more pronounced in wines aged with lees and stirred weekly. No significant differences were found in the concentration of biogenic amines in relation to the used maceration process.

  15. Oncoplastie avec conservation mammaire dans le traitement du cancer du sein: à propos de 16 cas

    Science.gov (United States)

    Bouzoubaa, Wail; Laadioui, Meryam; Jayi, Sofia; Alaoui, Fatime Zahra Fdili; Bouguern, Hakima; Chaara, Hikmat; Melhouf, Moulay Abdelilah

    2015-01-01

    Le cancer du sein est actuellement le cancer le plus fréquent chez la femme, et pose un véritable problème diagnostique et thérapeutique. Le dépistage des lésions à un stade de plus en plus précoce, a permis une extension des indications du traitement conservateur radiochirurgical, qui était initialement limitées aux tumeurs de moins de 3 cm, unifocales, non inflammatoires. Par ailleurs, l'utilisation de traitements préopératoires permet d’étendre les indications du traitement conservateur à des tumeurs plus volumineuses. Parallèlement à cette extension des indications de conservation mammaire, on a observé le développement de nouvelles approches thérapeutiques notamment la chirurgie oncoplastique, technique du ganglion sentinelle et chirurgie stéréotaxique, dont les résultats initiaux sont très encouragent. A travers cette étude réalisée dans le service de gynécologie et obstétrique II du CHU HASSAN II de FES au MAROC, après l'analyse rétrospective de 16 patientes traitées par traitement conservateur et oncoplastie, nous avons voulus montrer notre aptitude a réalisé ses techniques chirurgicales et a bien prendre en charge ces patientes, mais aussi évaluer ces techniques en termes de résultat carcinologique et de résultat esthétique, aussi en terme de survie globale, survie sans métastase et en termes de récidive locale entre les plasties mammaires et les traitements usuels: mastectomie et traitement conservateur classique. PMID:26430477

  16. Enhanced reactivities toward amines by introducing an imine arm to the pincer ligand: Direct coupling of two amines to form an imine without oxidant

    KAUST Repository

    He, Lipeng

    2012-07-23

    Dehydrogenative homocoupling of primary alcohols to form esters and coupling of amines to form imines was accomplished using a class of novel pincer ruthenium complexes. The reactivities of the ruthenium pincer complexes for the direct coupling of amines to form imines were enhanced by introducing an imine arm to the pincer ligand. Selective oxidation of benzylamines to imines was achieved using aniline derivatives as the substrate and solvent. © 2012 American Chemical Society.

  17. Conus venom peptide pharmacology.

    Science.gov (United States)

    Lewis, Richard J; Dutertre, Sébastien; Vetter, Irina; Christie, MacDonald J

    2012-04-01

    Conopeptides are a diverse group of recently evolved venom peptides used for prey capture and/or defense. Each species of cone snails produces in excess of 1000 conopeptides, with those pharmacologically characterized (≈ 0.1%) targeting a diverse range of membrane proteins typically with high potency and specificity. The majority of conopeptides inhibit voltage- or ligand-gated ion channels, providing valuable research tools for the dissection of the role played by specific ion channels in excitable cells. It is noteworthy that many of these targets are found to be expressed in pain pathways, with several conopeptides having entered the clinic as potential treatments for pain [e.g., pyroglutamate1-MrIA (Xen2174)] and one now marketed for intrathecal treatment of severe pain [ziconotide (Prialt)]. This review discusses the diversity, pharmacology, structure-activity relationships, and therapeutic potential of cone snail venom peptide families acting at voltage-gated ion channels (ω-, μ-, μO-, δ-, ι-, and κ-conotoxins), ligand-gated ion channels (α-conotoxins, σ-conotoxin, ikot-ikot, and conantokins), G-protein-coupled receptors (ρ-conopeptides, conopressins, and contulakins), and neurotransmitter transporters (χ-conopeptides), with expanded discussion on the clinical potential of sodium and calcium channel inhibitors and α-conotoxins. Expanding the discovery of new bioactives using proteomic/transcriptomic approaches combined with high-throughput platforms and better defining conopeptide structure-activity relationships using relevant membrane protein crystal structures are expected to grow the already significant impact conopeptides have had as both research probes and leads to new therapies. PMID:22407615

  18. Les élections cantonales du printemps 2011 en Alsace

    OpenAIRE

    Kleinschmager, Richard

    2012-01-01

    Le 30 mars 2011 Guy-Dominique Kennel a été réélu président du Conseil Général du département du Bas-Rhin. Trente-neuf conseillers généraux sur quarante quatre ont voté en sa faveur. Neuf d’entre eux ont porté leur voix sur Raphaël Nisand, nouveau conseiller général socialiste de Schiltigheim. Ce même jour à Colmar, vingt-et-un conseillers généraux du département du Haut-Rhin sur trente-et-un, réélisent Charles Buttner à la présidence de leur Conseil. Neuf voix ont opté pour Henri Stoll, le co...

  19. Festival du rire de Genève

    CERN Multimedia

    Staff Association

    2015-01-01

    Connaissez-vous le Festival du rire de Genève ? La deuxième édition aura lieu du 25 au 28 mars 2015 au Casino-Théâtre à Carouge. Côté programmation, Marc Donnet-Monay ouvre les festivités avant trois autres soirées de folie et d’humour que nous vous laissons le soin de découvrir dans le programme : http://www.rire-geneve.ch/#programme. Réduction de 30% sur l’achat de places pour les membres du personnel du CERN. Pour cela, il suffit de se rendre sur la billetterie en ligne de notre site : www.rire-geneve.ch et d’utiliser le code promotionnel. Contacter le secrétariat de l’Association du personnel (Staff.Association@cern.ch) pour connaitre ce code promotionnel.

  20. Modos de ver. Du Bois y Fanon

    Directory of Open Access Journals (Sweden)

    Sandro Mezzadra

    2014-07-01

    Full Text Available The sovereignty of glance and the primacy of sight represent the Western modern culture far beyond its artistic expression, organizing its philosophical and political conceptuality. They deeply influence the way in which subjects are conceived and constructed in Western modernity. This is the point of departure of the long-distance dialogue between W. E. B. Du Bois and Frantz Fanon staged in the article. Their political thought and militancy developed against the background of experiences – such as slavery and colonialism – characterized by process of construction of subjectivities which appear as “anomalous” with regard to the “standards” set by Modernity.

  1. Autour du mot comptabilité.

    OpenAIRE

    Pierre Labardin

    2006-01-01

    Le travail vise à comprendre l’origine du mot comptabilité à travers trois questions : d’abord, comment le mot s’est imposé et a remplacé l’expression tenue des livres ? Ensuite, comment l’ajout d’adjectif (commerciale, financière, industrielle etc.) a permis de classer le savoir comptable en plusieurs catégories et comment ces catégories ont évolué ? Enfin, quels sens recouvrent le mot comptabilité et quand ces sens sont apparus ?

  2. L’enfance au bord du Fjord

    OpenAIRE

    Durand, Frédéric

    2013-01-01

    Ce qui me plaît en vous, m’a-t-elle dit en me regardant longuement, ce qui me plaît en vous, je ne puis savoir pourquoi, ce sont mes souvenirs.Le Grand Meaulnes Le Jutland, terre des Cimbres Le Jutland, comme l’index brandi d’une main gigantesque, prolonge l’Europe continentale et sépare les lames grises et lourdes de la Mer du Nord des eaux vives de la Baltique. Sur cette langue de terre aux formes noueuses, se poursuivent et s’étreignent sans cesse, l’eau, la lande et le ciel, antique trini...

  3. Potential of phage-displayed peptide library technology to identify functional targeting peptides

    Science.gov (United States)

    Krumpe, Lauren RH; Mori, Toshiyuki

    2010-01-01

    Combinatorial peptide library technology is a valuable resource for drug discovery and development. Several peptide drugs developed through phage-displayed peptide library technology are presently in clinical trials and the authors envision that phage-displayed peptide library technology will assist in the discovery and development of many more. This review attempts to compile and summarize recent literature on targeting peptides developed through peptide library technology, with special emphasis on novel peptides with targeting capacity evaluated in vivo. PMID:20150977

  4. Amine synthesis via iron-catalysed reductive coupling of nitroarenes with alkyl halides.

    Science.gov (United States)

    Cheung, Chi Wai; Hu, Xile

    2016-01-01

    (Hetero)Aryl amines, an important class of organic molecules in medicinal chemistry, are most commonly synthesized from anilines, which are in turn synthesized by hydrogenation of nitroarenes. Amine synthesis directly from nitroarenes is attractive due to improved step economy and functional group compatibility. Despite these potential advantages, there is yet no general method for the synthesis of (hetero)aryl amines by carbon-nitrogen cross-coupling of nitroarenes. Here we report the reductive coupling of nitroarenes with alkyl halides to yield (hetero)aryl amines. A simple iron catalyst enables the coupling with numerous primary, secondary and tertiary alkyl halides. Broad scope and high functional group tolerance are demonstrated. Mechanistic study suggests that nitrosoarenes and alkyl radicals are involved as intermediates. This new C-N coupling method provides general and step-economical access to aryl amines. PMID:27515391

  5. In Situ Preparation of Polyether Amine Functionalized MWCNT Nanofiller as Reinforcing Agents

    Directory of Open Access Journals (Sweden)

    Ayber Yıldrım

    2014-01-01

    Full Text Available In situ preparation of polyether amine functionalized cross-linked multiwalled carbon nanotube (MWCNT nanofillers may improve the thermal and mechanical properties of the composites in which they are used as reinforcing agents. The reduction and functionalization of MWCNT using ethylenediamine in the presence of polyether amine produced stitched MWCNT's due to the presence of two amine (–NH2 functionalities on both sides of the polymer. Polyether amine was chosen to polymerize the carboxylated MWCNT due to its potential to form bonds with the amino groups and carboxyl groups of MWCNT which produces a resin used as polymeric matrix for nanocomposite materials. The attachment of the polyether amine (Jeffamine groups was verified by TGA, FT-IR, XRD, SEM, and Raman spectroscopy. The temperature at which the curing enthalpy is maximum, observed by DSC, was shifted to higher values by adding functionalized MWCNT. SEM images show the polymer formation between MWCNT sheets.

  6. Determination of oxyfluorfen herbicide and oxyfluorfen amine residues in garbanzo beans by liquid chromatography.

    Science.gov (United States)

    Zhou, M; Miles, C J

    1991-01-01

    Oxyfluorfen and oxyfluorfen amine were determined by liquid chromatography (LC) with ultraviolet (UV) and photoconductivity detection (PCD). A simple extraction procedure acceptably recovered both analytes from garbanzo beans over a wide range of fortifications (0.05 to 20 ppm) (83 +/- 4 for oxyfluorfen; 85 +/- 4 for oxyfluorfen amine). Percent recoveries decreased slightly as the fortification level decreased. Both analytes could be determined simultaneously at a concentration greater than 0.2 ppm in garbanzo beans. Detection limits were 3 ng for oxyfluorfen and 100 ng for oxyfluorfen amine using LC/UV, and 12 ng for both oxyfluorfen and oxyfluorfen amine with LC/PCD. Different knitted reaction coils and photoreactors were evaluated. Photoproduct yields and identification were determined by ion chromatography. The LC/PCD method measures oxyfluorfen and oxyfluorfen amine separately and has a shorter analysis time, while the standard method using gas chromatography measures total residues and is more sensitive. PMID:1874702

  7. Alteration of the Donor/Acceptor Spectrum of the (S-Amine Transaminase from Vibrio fluvialis

    Directory of Open Access Journals (Sweden)

    Maika Genz

    2015-11-01

    Full Text Available To alter the amine donor/acceptor spectrum of an (S-selective amine transaminase (ATA, a library based on the Vibrio fluvialis ATA targeting four residues close to the active site (L56, W57, R415 and L417 was created. A 3DM-derived alignment comprising fold class I pyridoxal-5′-phosphate (PLP-dependent enzymes allowed identification of positions, which were assumed to determine substrate specificity. These positions were targeted for mutagenesis with a focused alphabet of hydrophobic amino acids to convert an amine:α-keto acid transferase into an amine:aldehyde transferase. Screening of 1200 variants revealed three hits, which showed a shifted amine donor/acceptor spectrum towards aliphatic aldehydes (mainly pentanal, as well as an altered pH profile. Interestingly, all three hits, although found independently, contained the same mutation R415L and additional W57F and L417V substitutions.

  8. Platinum-Catalyzed, Terminal-Selective C(sp(3))-H Oxidation of Aliphatic Amines.

    Science.gov (United States)

    Lee, Melissa; Sanford, Melanie S

    2015-10-14

    This Communication describes the terminal-selective, Pt-catalyzed C(sp(3))-H oxidation of aliphatic amines without the requirement for directing groups. CuCl2 is employed as a stoichiometric oxidant, and the reactions proceed in high yield at Pt loadings as low as 1 mol%. These transformations are conducted in the presence of sulfuric acid, which reacts with the amine substrates in situ to form ammonium salts. We propose that protonation of the amine serves at least three important roles: (i) it renders the substrates soluble in the aqueous reaction medium; (ii) it limits binding of the amine nitrogen to Pt or Cu; and (iii) it electronically deactivates the C-H bonds proximal to the nitrogen center. We demonstrate that this strategy is effective for the terminal-selective C(sp(3))-H oxidation of a variety of primary, secondary, and tertiary amines. PMID:26439251

  9. Observation of the wrapping mechanism in amine carbon dioxide molecular interactions on heterogeneous sorbents.

    Science.gov (United States)

    Thirion, D; Rozyyev, V; Park, J; Byun, J; Jung, Y; Atilhan, M; Yavuz, C T

    2016-06-01

    Liquid, solvated amine based carbon capture is the core of all commercial or planned CO2 capture operations. Despite the intense research, few have looked systematically into the nature of amine molecules and their CO2 interaction. Here, we report a systematic introduction of linear ethylene amines on the walls of highly porous Davankov type network structures through simple bromination intermediates. Surprisingly, isosteric heats of CO2 adsorption show a clear linear trend with the increase in the length of the tethered amine pendant groups, leading to a concerted cooperative binding with additional H-bonding contributions from the unassociated secondary amines. CO2 uptake capacities multiply with the nitrogen content, up to an unprecedented four to eight times of the starting porous network under flue gas conditions. The reported procedure can be generalized to all porous media with the robust hydrocarbon framework in order to convert them into effective CO2 capture adsorbents. PMID:27174155

  10. Radiopharmaceutical development of radiolabelled peptides

    Energy Technology Data Exchange (ETDEWEB)

    Fani, Melpomeni; Maecke, Helmut R. [University Hospital Freiburg, Department of Nuclear Medicine, Freiburg (Germany)

    2012-02-15

    Receptor targeting with radiolabelled peptides has become very important in nuclear medicine and oncology in the past few years. The overexpression of many peptide receptors in numerous cancers, compared to their relatively low density in physiological organs, represents the molecular basis for in vivo imaging and targeted radionuclide therapy with radiolabelled peptide-based probes. The prototypes are analogs of somatostatin which are routinely used in the clinic. More recent developments include somatostatin analogs with a broader receptor subtype profile or with antagonistic properties. Many other peptide families such as bombesin, cholecystokinin/gastrin, glucagon-like peptide-1 (GLP-1)/exendin, arginine-glycine-aspartic acid (RGD) etc. have been explored during the last few years and quite a number of potential radiolabelled probes have been derived from them. On the other hand, a variety of strategies and optimized protocols for efficient labelling of peptides with clinically relevant radionuclides such as {sup 99m}Tc, M{sup 3+} radiometals ({sup 111}In, {sup 86/90}Y, {sup 177}Lu, {sup 67/68}Ga), {sup 64/67}Cu, {sup 18}F or radioisotopes of iodine have been developed. The labelling approaches include direct labelling, the use of bifunctional chelators or prosthetic groups. The choice of the labelling approach is driven by the nature and the chemical properties of the radionuclide. Additionally, chemical strategies, including modification of the amino acid sequence and introduction of linkers/spacers with different characteristics, have been explored for the improvement of the overall performance of the radiopeptides, e.g. metabolic stability and pharmacokinetics. Herein, we discuss the development of peptides as radiopharmaceuticals starting from the choice of the labelling method and the conditions to the design and optimization of the peptide probe, as well as some recent developments, focusing on a selected list of peptide families, including somatostatin

  11. Effect of foamability index of short chain alkyl amines on flotation of quartz

    Directory of Open Access Journals (Sweden)

    Szczerkowska Sabina

    2016-01-01

    Full Text Available Amines can be used for flotation of various minerals, especially quartz. The flotation efficiency of quartz depends on the amine type and dose. It was proved that the shorter alkyl amine, higher amine concentration has to be used to recover quartz at the same level. In flotation amines play a role of both collectors and frothers. The ability of a amine to collect particles can be expressed in the form of contact angle, while the foaming properties by different parameters including dynamic foamability index (DFI and critical coalescence concentration (CCC. Determination of DFI and CCC requires advanced techniques and methods. Therefore, in this paper a rapid and facile method for determination of foaming properties of amines and also other surfactants was used. It was based on measuring the initial foam and froth heights in a conventional flotation machine at different concentrations of surfactants. The foam height-concentration curve was described by utilizing an empirical equation which was based on one-adjustable parameter called the foamability index (FI. In this work the foamability index was determined for butylamine (ButNH2, hexylamine (HexNH2 and octylamine (OctNH2 as examples of short chain alkyl amines. The determined foamability indices were 92, 12 and 4 mg/dm3 for ButNH2, HexNH2 and OctNH2, respectively. It was shown that when the flotation results of quartz were presented in the form of recovery versus normalized amine concentration in relation to the foamability index (c/FI, all the experimental data points converged to one curve. It indicates that amines act similarly but at different concentrations expressed as FI. The foamability index seems to be a useful parameter for characterizing any flotation frother.

  12. Nestor Kirchner (2003-2007) : rupture ou continuite du peronisme ? Les mutations du populisme en Argentine

    OpenAIRE

    Doz, Emilie

    2015-01-01

    Ce texte est un extrait du quatrième chapitre de la thèse « Néstor Kirchner (2003-2007) : Rupture ou continuité du péronisme. Les mutations du populisme en Argentine ». Le 25 mai 2003, le peuple argentin assistait à l’investiture présidentielle du candidat du Frente Para la Victoria, Néstor Kirchner. Suite à la défection de Carlos Menem pendant l’entre-deux tours, il est élu avec 22% des suffrages. Malgré cette faible légitimité, il réussit à insuffler un changement certain dans la politique ...

  13. Peptide primary messengers in plants

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The peptide primary messengers regulate embryonic development,cell growth and many other activities in animal cells. But recent evidence verified that peptide primary messengers are also involved in plant defense responses, the recognition between pollen and stigma and keep the balance between cell proliferation and differentiations in shoot apical meristems. Those results suggest that plants may actually make wide use of peptide primary messengers, both in embryonic development and late life when they rally their cells to defend against pathogens and insect pests. The recent advance in those aspects is reviewed.

  14. 40 CFR 721.3830 - Formaldehyde, reaction products with an alkylated phenol and an aliphatic amine (generic).

    Science.gov (United States)

    2010-07-01

    ... an alkylated phenol and an aliphatic amine (generic). 721.3830 Section 721.3830 Protection of... products with an alkylated phenol and an aliphatic amine (generic). (a) Chemical substance and significant..., reaction products with an alkylated phenol and an aliphatic amine (PMN P-99-0531) is subject to...

  15. Du Bois的对话句法

    Institute of Scientific and Technical Information of China (English)

    高彦梅

    2015-01-01

    日常口语交流过程中,人们会自觉不自觉地复用即时语境中前面说话人或历史上前人的词语、短语、句法结构等成分。这样便在自己和前面说话人的话语之间唤起了共鸣。Du Bois在Bakhtin的对话理论、Jakobson的平行理论和Harris的话语分析程序的启发之下,依据自然口语语料、从社会认知角度发展了对话句法理论,目的在于揭示人类交流过程中符号与符号之间并通过符号表达的介入结构。本文综合Du Bois以及相关学者过去三十年间对对话句法的研究,呈现对话句法理论的主体脉络。

  16. The Pic du Midi solar survey

    Science.gov (United States)

    Koechlin, L.

    2015-12-01

    We carry a long term survey of the solar activity with our coronagraphic system at Pic du Midi de Bigorre in the French Pyrenees (CLIMSO). It is a set of two solar telescopes and two coronagraphs, taking one frame per minute for each of the four channels : Solar disk in H-α (656.28 nm), prominences in H-α, disk in Ca II (393.3 nm), prominences in He I (1083 nm), all year long, weather permitting. Since 2015 we also take images of the FeXIII corona (1074.7 nm) at the rate of one every 10 minutes. These images cover a large field: 1.25 solar diameter, 2k*2K pixels, and are freely downloadable form a database. The improvements made since 2015 concern an autoguiding system for better centering of the solar disk behind the coronagraphic masks, and a new Fe XIII channel at λ=1074.7 nm. In the near future we plan to provide radial velocity maps of the disc and polarimetry maps of the disk and corona. This survey took its present form in 2007 and we plan to maintain image acquisition in the same or better experimental conditions for a long period: one or several solar cycles if possible. During the partial solar eclipse of March 20, 2015, the CLIMSO instruments and the staff at Pic du Midi operating it have provided several millions internet users with real time images of the Sun and Moon during all the phenomenon.

  17. La mathématique du physicien

    CERN Document Server

    Diu, Bernard

    2012-01-01

    " Le livre de la nature est écrit dans la langue mathématique, et ses caractères sont des triangles, des cercles et autres figures géométriques, sans lesquels il est humainement impossible d'en comprendre le moindre mot ". Ce célèbre aphorisme de Galilée a scellé l'alliance des mathématiques et de la physique. Et pourtant, trois siècles plus tard, Einstein se montrait plus sceptique. Dans ce livre, Bernard Diu montre que, en effet, si les mathématiques sont un instrument indispensable de la physique, elles n'en constituent pas le fondement. En multipliant les exemples, il marque la différence entre la mathématique du mathématicien et celle du physicien. Un livre de nature à remettre en question l'enseignement de la physique dans nos écoles et des sciences en général, soumis à l'hégémonie des mathématiques.

  18. Screening of TACE Peptide Inhibitors from Phage Display Peptide Library

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    To obtain the recombinant tumor necrosis factor-α converting enzyme (TACE) ectodomain and use it as a selective molecule for the screening of TACE peptide inhibitors, the cDNA coding catalytic domain (T800) and full-length ectodomain (T1300) of TACE were amplified by RTPCR, and the expression plasmids were constructed by inserting T800 and T1300 into plasmid pET28a and pET-28c respectively. The recombinant T800 and T1300 were induced by IPTG, and SDSPAGE and Western blotting analysis results revealed that T800 and T1300 were highly expressed in the form of inclusion body. After Ni2+-NTA resin affinity chromatography, the recombinant proteins were used in the screening of TACE-binding peptides from phage display peptide library respectively. After 4 rounds of biopanning, the positive phage clones were analyzed by ELISA, competitive inhibition assay and DNA sequencing. A common amino acid sequence (TRWLVYFSRPYLVAT) was found and synthesized. The synthetic peptide could inhibit the TNF-α release from LPS-stimulated human peripheral blood mononuclear cells (PBMC) up to 60.3 %. FACS analysis revealed that the peptide mediated the accumulation of TNF-α on the cell surface. These results demonstrate that the TACE-binding peptide is an effective antagonist of TACE.

  19. Pour l'effectivité du droit du travail : quel système d'inspection et quelle indépendance des inspecteurs ?

    OpenAIRE

    Auvergnon, Philippe

    2006-01-01

    L'effectivité du droit du droit du travail est-elle réellement possible dans un système généraliste d'inspection du travail couvrant l'ensemble des secteurs ? Ou plutôt trouve-t-elle sa place dans un système indépendant reposant sur un fondement normatif dans lequel le contrôle s'exerce loin de toute influence extérieure pouvant nuire à la réalité et l'efficacité de la mission fondamentale d'application du droit du travail ?

  20. Développement de l'étuvage du riz au Bénin

    OpenAIRE

    Houssou, Paul

    2003-01-01

    International audience L'étuvage du riz paddy est une opération qui entraîne des modifications physico-chimiques et organoleptiques avantageuses du point de vue nutritionnel et économique. Au Bénin, la technique d'étuvage est pratiquée par les transformatrices du centre (zone des collines) et du nord du pays. Ces transformatrices utilisent le trempage du paddy à chaud avec pré-cuisson "étuvage" dans l'eau contenue dans une marmite en fonte d'aluminium. Malheureusement, la qualité du riz ca...