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Sample records for ameloblastoma

  1. Cystic Granular Cell Ameloblastoma

    OpenAIRE

    Thillaikarasi, Rathnavel; Balaji, Jayaram; Gupta, Bhawna; Ilayarja, Vadivel; Vani, Nandimandalam Venkata; Vidula, Balachander; Saravanan, Balasubramaniam; Ponniah, Irulandy

    2010-01-01

    Ameloblastoma is a locally aggressive benign epithelial odontogenic tumor, while unicystic ameloblastoma is a relatively less aggressive variant. Although rare in unicystic or cystic ameloblastoma, granular cell change in ameloblastoma is a recognized phenomenon. The purpose of the present article is to report a case of cystic granular cell ameloblastoma in 34-year old female.

  2. Ameloblastoma solido/multiquistico

    OpenAIRE

    Alemán Navas, Ramos Manuel; Martínez Mendoza, María Guadalupe

    2008-01-01

    El ameloblastoma es una neoplasia benigna, agresiva, de crecimiento lento que puede ser más destructivo y amenazador para la vida que algunos de los tumores malignos de la región oral y maxilofacial específicamente hablando del ameloblastoma solido/multiquistico o del ameloblastoma unquístico mural. El ameloblastoma es el tumor odontogénico benigno más frecuente en muchos países del mundo

  3. Desmoplastic ameloblastoma of mandible.

    Science.gov (United States)

    Nair, Preeti P; Bhat, Guruprasad R; Neelakantan, Shiba; Chatterjee, Rhiti

    2013-09-17

    Desmoplastic ameloblastoma is one of the six histopathological subtypes of ameloblastoma. The age and gender groups affected by desmoplastic are similar to those affected by the conventional ameloblastoma. It usually presents as a painless enlargement of the jaw. Owing to its deceptive radiological appearance as a mixed radiopaque-radiolucent lesion, it is often mistaken as a fibro-osseous lesion. Histologically, desmoplastic ameloblastoma has a densely collagenised and hypocellular stroma, where the epithelium tends to proliferate in the form of cords and nests instead of cellular islands. Most desmoplastic ameloblastomas display occasional classic islands of follicular ameloblastoma among the predominant strands and cords. Studies have shown that desmoplastic ameloblastoma shows a tendency to recur. We present a rare case of a tumour occurring in the anterior mandibular region in a 60-year-old man over a period of 11/2 months.

  4. Ameloblastoma desmoplásico Desmoplastic ameloblastoma

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    J.L. Martínez-Lage

    2010-03-01

    Full Text Available Presentamos un caso de ameloblastoma desmoplásico en una mujer de 32 años que afecta a la región media del maxilar superior. Se describen las características específicas, tanto histológicas como clínicas, de esta variante de ameloblastoma, y se insiste en su imagen radiológica similar a la de una lesión fibroósea benigna. Se indica la misma conducta terapéutica que para el resto de los ameloblastomas intraóseos sólidos.We report a case of desmoplastic ameloblastoma in a 32 year-old female patient affecting the region half of the upper jaw. We describe the specific characteristics, both histological and clinical of this variant of ameloblastoma, insisting its radiological image similar to that of a benign fibroosseous lession. We indicate the same therapeutic conduct for the rest of the solid intraosseous ameloblastomas.

  5. Hybrid ameloblastoma: An amalgam of rare and conventional ameloblastoma

    OpenAIRE

    Chaitanya, B.; Chhaparwal, Yogesh; Pai, Keerthilatha M.; Kudva, Adarsh; Cariappa, K. M.; Acharya, Shruthi

    2016-01-01

    Ameloblastoma is a common benign odontogenic tumor with multiple histologic types. This case report describes an unusual type of ameloblastoma called “Hybrid Ameloblastoma” with features of both follicular and desmoplastic ameloblastoma in a 50-year-old female. This is a very rare form of ameloblastoma as

  6. Cytologic picture of ameloblastoma

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    Bokun Radojka

    2003-01-01

    Full Text Available Ameloblastoma is a rare tumor of the jaw arising from odontogenic epithelium. There are sparse reports in the literature concerning cytologic features of this tumor. This paper presents two cases of ameloblastoma diagnosed by imprint cytology and confirmed histopathologically. The imprints were hypercellular, with single cells and the groups of basaloid and polygonal squamous cells with huge vacuoles in cytoplasm. Stellate and fusiform cells were found in the background of the preparation. These morphologic parameters were sufficient for the cytologic diagnosis of ameloblastoma.

  7. Ameloblastoma in children; Ameloblastoma u dzieci

    Energy Technology Data Exchange (ETDEWEB)

    Korzon, M.; Popadiuk, S.; Bohdan, Z. and others [Akademia Medyczna, Gdansk (Poland)

    1993-12-31

    Two cases of ameloblastoma in 3 and 12 year old children are described. The difficulties in the diagnosis and treatment of this rare tumor of the jaws (mandibula) are presented. Only a short remission following chemotherapy was achieved in both cases. (author) 19 refs, 2 figs

  8. Malignant ameloblastoma or ameloblastic carcinoma

    NARCIS (Netherlands)

    Slootweg, P.J.; Müller, H.

    1984-01-01

    The World Health Organization defines malignant ameloblastoma as a lesion exhibiting features of an ameloblastoma in primary and metastatic growths. To cases collected from the literature we have added two of our own cases in which features of an ameloblastoma were coupled with malignant behavior.

  9. Granular cell ameloblastoma of mandible.

    Science.gov (United States)

    Jansari, Trupti R; Samanta, Satarupa T; Trivedi, Priti P; Shah, Manoj J

    2014-01-01

    Ameloblastoma is a neoplasm of odontogenic epithelium, especially of enamel organ-type tissue that has not undergone differentiation to the point of hard tissue formation. Granular cell ameloblastoma is a rare condition, accounting for 3-5% of all ameloblastoma cases. A 30-year-old female patient presented with the chief complaint of swelling at the right lower jaw region since 1 year. Orthopantomogram and computed tomography scan was suggestive of primary bone tumor. Histopathologically, diagnosis of granular cell ameloblastoma of right mandible was made.

  10. Recurrent Ameloblastoma: A Surgical Challenge

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    Chithra Aramanadka

    2018-01-01

    Full Text Available Ameloblastoma is locally aggressive benign odontogenic tumour with increased risk of recurrence rate. The choice of treatment depends on the histologic subtype. Radical therapy is the recommended modality for solid ameloblastomas. The possibilities of recurrence even after enbloc resection are still high. The author presents two case reports of recurrent ameloblastomas postradical resection. First case describes the recurrence of ameloblastoma in the bone graft which was used for reconstruction, and the second case depicts recurrence in the infratemporal fossa. Intraoperative radiography of the frozen section of the soft tissue margin plays an important role in the holistic management of these lesions.

  11. Microgenomics of Ameloblastoma

    Science.gov (United States)

    DeVilliers, P.; Suggs, C.; Simmons, D.; Murrah, V.; Wright, J.T.

    2011-01-01

    Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets. PMID:21282726

  12. Treatment of ameloblastoma

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    Fernando Vagner Raldi

    2010-04-01

    Full Text Available Ameloblastoma is a benign odontogenic tumor of epithelial origin, with slow growth, frequently found in the mandible or maxilla. The symptoms are unnoticeable, rarely being perceived by the patient in the initial stages, and are sometimes diagnosed by chance in routine radiographic exams. Clinically, it is a hardened curvature that grows slowly, and the regional mucosa remains with the normal appearance. There is rarely a solution of continuity or infection. The ameloblastoma invades the spaces between the trabeculated bones without reabsorbing them, and expands the compact bone without invading it, however, it may propagate through the mandibular canal. It has been given this name due to its similarity to the cells of the enamel organ and it has a unique biological behavior that leads to controversy with regard to the best form of treatment. This clinical case describes the case of a unicystic ameloblastoma located in the left retromandibular region with eight years of follow-up. Clinical and radiographic diagnosis was confirmed through the histopathologic exam. Treatment proposed was enucleation without marginal dissection.

  13. Overwhelming hypercalcaemia in mandibular ameloblastoma.

    Science.gov (United States)

    Lo, Tom Edward Ngo; Villafuerte, Cesar Vincent; Acampado, Laura Trajano

    2014-10-17

    Ameloblastoma is considered to be a benign odontogenic tumour of epithelial in origin that is slow growing but recurrent and invasive in nature. Some of its features have been sources of debate among experts regarding its benign or malignant character. We report a case of a 20-year-old Filipino woman with right mandibular ameloblastoma presenting with overwhelming hypercalcaemia. Work ups for hypercalcaemia eventually revealed tumoral hypercalcaemia, which was initially controlled with intravenous bisphosphanate. The patient eventually underwent tumour excision and mandibular reconstruction, which totally corrected hypercalcaemia. This case will highlight the rare association of hypercalcaemia among patients with ameloblastoma. 2014 BMJ Publishing Group Ltd.

  14. Acanthomatous ameloblastoma: A rare presentation

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    Ramaswamy Pachigolla

    2016-01-01

    Full Text Available Ameloblastoma is the most frequent odontogenic tumor arising from dental epithelium. It is characterized by its histological resemblance to the enamel organ of the developing tooth germ; but enamel formation is not observed. Acanthomatous ameloblastoma is considered an aggressive odontogenic tumor characterized by irregular verrucous masses adjacent to the tooth, consisting of sheets of keratinizing odontogenic epithelium. Here we are reporting a case of 35-year-old male with swelling on left body of the mandible region which was diagnosed as acanthomatous ameloblastoma based on clinical, radiographic and histopathological features.

  15. Computed Tomographic Evaluation of Mandibular Ameloblastoma

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    N Eswar

    2003-01-01

    Five interesting cases of mandibular ameloblastoma are presented here, each case showing different histological pattern and corresponding computer tomographic appearance. Also an attempt is made to establish CT pattern in these histological varieties of ameloblastoma.

  16. Plexiform Unicystic Ameloblastoma: A Rare Variant of Ameloblastoma

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    Swapnil S. Deore

    2014-01-01

    Full Text Available The term plexiform unicystic ameloblastoma refers to a pattern of epithelial proliferation that has been described in cystic cavity. Because of unilocular presentation, it is commonly misdiagnosed as an odontogenic cyst. However, they may often behave clinically as biologically aggressive tumors. These tumors show high incidence of cortical perforation, tooth resorption and a high rate of recurrence after simple enucleation. This paper aims to provide an insight into this biologically distinct entity. A literature review on the topic has been added along with a case report highlighting the approach of diagnosis and management of such ameloblastomas.

  17. An infrequent histopathological subtype of ameloblastoma: Adenoid granular cell ameloblastoma with dentinoid

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    Jahanshah Salehinejad

    2016-01-01

    Full Text Available Adenoid ameloblastoma with dentinoid is a rare odontogenic tumor. Granular cell ameloblastoma also is a less common histological subtype of ameloblastoma. In this report, the patient was a 31-year-old male. The lesion was located in the right mandible and was unicystic with well-defined borders. The tumor tissue was showing a combination of follicular, plexiform, and desmoplastic patterns of ameloblastoma with wide areas of granular cells, fibrous stroma, glandular pattern, and dentinoid calcified. Very few cases of distinct forms of ameloblastoma that show the formation of dentinoid has been reported. However, there are no cases of adenoid granular cell ameloblastoma with dentinoid reported.

  18. Plexiform Ameloblastoma of the Mandible

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    Dinesh Singh Chauhan

    2011-01-01

    Full Text Available Ameloblastoma is a common and aggressive odontogenic epithelial tumor. It has an aggressive behavior and recurrent course, and is rarely metastatic. Ameloblastoma represents 1% of all tumors and cysts that involve the maxillomandibular area and about 10% of the odontogenic tumors. It is primarily seen in adults in the third to fifth decade of life, with equal sex predilection. Radiographically, it appears as an expansile radiolucent, with thinned and perforated cortices, and is known to cause root resorption. As it shares common radiographic features with other lesions such as the giant cell tumor, aneurismal bone cyst, and renal cell carcinoma metastasis, a definitive diagnosis can only be made with histopathology. We present an extensive case of plexiform ameloblastoma of the mandible in a 42-year-old female patient.

  19. Ameloblastoma, Solid/Multicystic Type

    Science.gov (United States)

    Bachmann, Angela M.

    2009-01-01

    The ameloblastoma, particularly the solid/multicystic type, is the most clinically significant odontogentic tumor. The tumor is often locally aggressive and has a significant impact and may have a patient’s morbidity and mortality. In this report, we present a case of a large ameloblastoma that presented with the typical radiographic features of variably sized radiolucent loculations. Microscopically the tumor showed a variety of histologic types, with plexiform and follicular predominating. The tumor was treated with a partial resection of the mandible. PMID:20596851

  20. Tratamento cirúrgico de ameloblastoma mandibular

    OpenAIRE

    Jardim, Ellen Cristina Gaetti; Faverani, Leonardo Peres [UNESP; Melo, Rosana Leite de; Miyahara, Glauco Issamu [UNESP; Soubhia, Ana Maria Pires [UNESP; Mendonça, José Carlos Garcia de; Shinohara, Elio Hitoshi [UNESP

    2013-01-01

    Ameloblastomas are benign, invasive locally and highly recurrent. It is an odontogenic tumor, characterized by the proliferation of epithelial ameloblastic in a fibrous stroma. This paper reports a case of mandibular ameloblastoma, in patients 27 years of age without pain with developments around 4 years, with about 20 mm at its greatest extent, sessile base and surface coatings full. The treatment of choice was the surgical conservative Ameloblastomas son benignos, localmente invasivo y a...

  1. Expression of RECK and matrix metalloproteinase-2 in ameloblastoma

    Science.gov (United States)

    2009-01-01

    Background Ameloblastoma is a frequent odontogenic benign tumor characterized by local invasiveness, high risk of recurrence and occasional metastasis and malignant transformation. Matrix metalloproteinase-2 (MMP-2) promotes tumor invasion and progression by destroying the extracellular matrix (ECM) and basement membrane. For this proteolytic activity, the endogenous inhibitor is reversion-inducing cysteine rich protein with Kazal motifs (RECK). The aim of this study was to characterize the relationship between RECK and MMP-2 expression and the clinical manifestation of ameloblastoma. Methods Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were employed to detect the protein and mRNA expression of RECK and MMP-2 in keratocystic odontogenic tumor (KCOT), ameloblastoma and ameloblastic carcinoma. Results RECK protein expression was significantly reduced in KCOT (87.5%), ameloblastoma (56.5%) and ameloblastic carcinoma (0%) (P ameloblastoma compared with primary ameloblastoma (P ameloblastoma. MMP-2 protein expression was significantly higher in ameloblastoma and ameloblastic carcinoma compared with KCOT (P ameloblastoma than in KCOT (P ameloblastoma than in primary ameloblastoma, and was negative in ameloblastic carcinoma. MMP-2 mRNA expression was significantly higher in ameloblastoma compared with KCOT (P ameloblastoma versus primary ameloblastoma. RECK protein expression was negatively associated with MMP-2 protein expression in ameloblastoma (r = -0.431, P ameloblastoma. RECK may participate in the invasion, recurrence and malignant transformation of ameloblastoma by regulating MMP-2 at the post-transcriptional level. PMID:19995435

  2. Ameloblastoma: an aggressive lesion of the mandible.

    Science.gov (United States)

    Suma, M S; Sundaresh, K J; Shruthy, R; Mallikarjuna, Rachappa

    2013-10-09

    Ameloblastoma is a benign locally invasive epithelial odontogenic tumour comprising 1% of all tumours and cysts arising in the jaws. It is commonly found in the third and fourth decade in the molar ramus region of the mandible. Among all types of ameloblastoma, multicystic ameloblastoma is believed to be locally aggressive lesion that has the tendency for recurrence. In this report we present a large multicystic ameloblastoma in the left body-ramus region of the mandible in a 55-year-old woman. This large lesion was diagnosed with the help of CT and was successfully managed by hemimandibulectomy with simultaneous reconstruction using iliac crest bone.

  3. Manejo quirúrgico del ameloblastoma Surgical management of ameloblastoma

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    Adaia Valls

    2012-09-01

    Full Text Available El ameloblastoma es un tumor odontogénico de estirpe epitelial. Aunque se clasifica como una tumoración benigna, suele ser localmente agresiva presentando elevada invasión local, con gran tendencia a la recidiva y con posibilidad metastásica ocasional. Se manifiesta preferentemente durante la tercera, cuarta y quinta décadas de la vida, sin predilección por razón de sexo, aunque puede darse en cualquier grupo de edad, incluidos los niños. La mayoría de los ameloblastomas se encuentran sobre todo en mandíbula (al nivel del ángulo y rama. En el tratamiento se debe valorar su tipología clínica (sólido, multiquístico, uniquístico, mixto o periférico, su localización y el tamaño del tumor, así como la edad y las condiciones clínicas del paciente. Presentamos una revisión de los pacientes afectos de ameloblastomas tratados en nuestro Centro durante los últimos 10 años. Se aportan datos acerca de su aparición clínica, sus características histológicas, el manejo terapéutico realizado y analizamos el seguimiento y comparamos la aparición de recidivas en los pacientes presentados. Las características clínicas, incluso si se complementan con radiografías y/o muestras histológicas, no son siempre determinantes del comportamiento biológico y, por tanto tampoco lo son del pronóstico de un ameloblastoma individual.The ameloblastoma is an odontogenic tumour of epithelial origin. Although it is classified as benign, there is usually aggressive local invasion, a great tendency to recurrence, and occasional metastatic potential. It generally appears during the third, fourth and fifth decades of life, without gender predilection, although it can occur at any age, including in children. Ameloblastomas are mostly found in the mandible (angle and branch. In treatment, its clinical type (solid, unicystic, desmoplastic, mixed or peripheral, its location and size, must be assessed, as well as the age and clinical condition of the

  4. Multilocular Unicystic Ameloblastoma of Mandible

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    Manas Bajpai

    2013-01-01

    Full Text Available Introduction. We report a rare case of unicystic ameloblastoma (UA of mandible which showed multilocular radiolucency on the left side of mandible on radiographic examination which is very unusual, and the majority of the cases of UAs till date has been reported of unilocular radiolucency. On histopathological examination, an odontogenic cystic lining that proliferates that intraluminally resembling ameloblastomatous epithelium was observed, leading to a definitive diagnosis of unicystic ameloblastoma. Case Presentation. A 42-year-old male patient presented with a swelling on the left side of the mandible extending from 33 to 36. Radiographically, it showed a multilocular radiolucent lesion resembling odontogenic cyst; however, the final diagnosis was made on histopathological ground with the inclusion of radiological and clinical features. Conclusion. It can be concluded that at present, histopathologic examination is the most sensitive tool for differentiating between odontogenic cysts and UAs. However, both clinical and radiologic findings share equal contribution to the final diagnosis.

  5. [Diagnosis and treatment of ameloblastoma].

    Science.gov (United States)

    Paredes Díaz, J C; Vergara Piedra, L

    1989-01-01

    In the light of current knowledge, a discussion is made on 10 patients with ameloblastoma operated on in Josina Machel General Hospital, in the People's Republic of Angola. This tumor, which has a high incidence in that country, can be operated on in spite of the shortage of a vailable resources, by means of an autogenous graft. Age, sex, location, course of the disease, and results in this group of patients are discussed.

  6. [Unicystic ameloblastoma as a clinicopathologic entity].

    Science.gov (United States)

    Aguirre Urízar, J M; González Tanago, J; Fernández de Larrinoa, A; Vallejo Irastorza, G; Martínez Conde, R

    1991-02-01

    Due to its clinic and histopathologic features, the unicystic ameloblastoma is a special kind of ameloblastoma, and it has a more favourable prognosis. The more interesting clinicopathologic aspects and the importance of a correct diagnosis are discussed in this paper, where we report two new mandibular cases of this tumor in young patients.

  7. CYSTIC AMELOBLASTOMA: A CLINICO-PATHOLOGIC REVIEW

    African Journals Online (AJOL)

    a tertiary health care centre. Materials: All cases diagnosed as cystic ameloblastoma in the ..... Unicystic amelobla- stoma. A prognostically distinct entity. Cancer. 1977;40: 2278-2285. 4. Ackermann GL, Altini M, Shear M: The unicystic ameloblastoma: A clinicopathologic study of 57 cases. J Oral Pathol. 1988;17: 541-546. 5.

  8. Unicystic ameloblastoma arising from a residual cyst

    Science.gov (United States)

    Mahajan, Amit D; Manjunatha, Bhari Sharanesha; Khurana, Neha M; Shah, Navin

    2014-01-01

    Intraoral swellings involving alveolar ridges in edentulous patients are clinically diagnosed as residual cysts, traumatic bone cysts, Stafne's jaw bone cavity, ameloblastoma and metastatic tumours of the jaw. This case report describes a residual cyst in a 68-year-old edentulous male patient which was enucleated and histopathologically confirmed as a unicystic ameloblastoma. PMID:25199192

  9. Granular cell ameloblastoma: case report of a particular ameloblastoma histologically resembling oncocytoma.

    Science.gov (United States)

    Matsushita, Yuki; Fujita, Shuichi; Kawasaki, Goro; Hirota, Yoshinosuke; Rokutanda, Satoshi; Yamashita, Kentaro; Yanamoto, Souichi; Ikeda, Tohru; Umeda, Masahiro

    2015-01-01

    Granular cell ameloblastoma is classified as a histological subtype of solid/multicystic ameloblastoma. Usual granular cell ameloblastoma is histologically characterized by granular changes of stellate-like cells located in the inner portion of the epithelial follicles. Here we report a case of another type of granular cell ameloblastoma, showing predominant anastomosing double-stranded trabeculae of granular cells. This type of granular cell ameloblastoma is extremely rare, and the World Health Organization classification does not contain the entity. We tentatively termed it 'anastomosing granular cell ameloblastoma' in this report. The present case suggests the importance of differential diagnosis because the histology of 'anastomosing granular cell ameloblastoma' resembles that of salivary gland oncocytoma rather than that of usual granular cell ameloblastoma. The trabeculae observed in our case continued to the peripheral cells of a small amount of epithelial sheets of plexiform ameloblastoma, and the tumor cells were positive for CK19, which is regarded as an immunohistochemical marker of odontogenic epithelium. Similar to usual granular cell ameloblastoma, the tumor cells had CD68-positive granules. For precise diagnosis of this condition, immunohistochemistry using CK19 and CD68, as well as detailed histological observation, are recommended. © 2014 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.

  10. Incidence and overall survival of malignant ameloblastoma.

    Science.gov (United States)

    Rizzitelli, Alexandra; Smoll, Nicolas R; Chae, Michael P; Rozen, Warren M; Hunter-Smith, David J

    2015-01-01

    Malignant ameloblastoma, comprising metastasizing ameloblastoma and ameloblastic carcinoma, represents 1.6-2.2% of all odontogenic tumors. Due to its rare nature, malignant ameloblastoma has only been reported in the literature in small case series or case reports. Using the Surveillance, Epidemiology and End-Results (SEER) database, we have performed a population-based study to determine the incidence rate and the absolute survival of malignant ameloblastoma. Using the International Classification of Diseases for Oncology (ICD-O) codes 9310/3 and 9270/3, data from the SEER database were used to calculate the incidence rate and absolute survival rate of population with malignant ameloblastoma. The overall incidence rate of malignant ameloblastoma was 1.79 per 10 million person/year. The incidence rate was higher in males than females and also higher in black versus white population. The median overall survival was 17.6 years from the time of diagnosis and increasing age was associated with a statistically significant poorer survival. To our best knowledge, we report the largest population-based series of malignant ameloblastoma. The incidence rate was 1.79 per 10 million person/year and the overall survival was 17.6 years.

  11. Incidence and overall survival of malignant ameloblastoma.

    Directory of Open Access Journals (Sweden)

    Alexandra Rizzitelli

    Full Text Available Malignant ameloblastoma, comprising metastasizing ameloblastoma and ameloblastic carcinoma, represents 1.6-2.2% of all odontogenic tumors. Due to its rare nature, malignant ameloblastoma has only been reported in the literature in small case series or case reports. Using the Surveillance, Epidemiology and End-Results (SEER database, we have performed a population-based study to determine the incidence rate and the absolute survival of malignant ameloblastoma.Using the International Classification of Diseases for Oncology (ICD-O codes 9310/3 and 9270/3, data from the SEER database were used to calculate the incidence rate and absolute survival rate of population with malignant ameloblastoma.The overall incidence rate of malignant ameloblastoma was 1.79 per 10 million person/year. The incidence rate was higher in males than females and also higher in black versus white population. The median overall survival was 17.6 years from the time of diagnosis and increasing age was associated with a statistically significant poorer survival.To our best knowledge, we report the largest population-based series of malignant ameloblastoma. The incidence rate was 1.79 per 10 million person/year and the overall survival was 17.6 years.

  12. Expression of RECK and matrix metalloproteinase-2 in ameloblastoma

    International Nuclear Information System (INIS)

    Zhang, Bin; Zhang, Jin; Xu, Zhi-Ying; Xie, Hong-Liang

    2009-01-01

    Ameloblastoma is a frequent odontogenic benign tumor characterized by local invasiveness, high risk of recurrence and occasional metastasis and malignant transformation. Matrix metalloproteinase-2 (MMP-2) promotes tumor invasion and progression by destroying the extracellular matrix (ECM) and basement membrane. For this proteolytic activity, the endogenous inhibitor is reversion-inducing cysteine rich protein with Kazal motifs (RECK). The aim of this study was to characterize the relationship between RECK and MMP-2 expression and the clinical manifestation of ameloblastoma. Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were employed to detect the protein and mRNA expression of RECK and MMP-2 in keratocystic odontogenic tumor (KCOT), ameloblastoma and ameloblastic carcinoma. RECK protein expression was significantly reduced in KCOT (87.5%), ameloblastoma (56.5%) and ameloblastic carcinoma (0%) (P < 0.01), and was significantly lower in recurrent ameloblastoma compared with primary ameloblastoma (P < 0.01), but did not differ by histological type of ameloblastoma. MMP-2 protein expression was significantly higher in ameloblastoma and ameloblastic carcinoma compared with KCOT (P < 0.01). RECK mRNA expression was significantly lower in ameloblastoma than in KCOT (P < 0.01), lower in recurrent ameloblastoma than in primary ameloblastoma, and was negative in ameloblastic carcinoma. MMP-2 mRNA expression was significantly higher in ameloblastoma compared with KCOT (P < 0.01), but was no different in recurrent ameloblastoma versus primary ameloblastoma. RECK protein expression was negatively associated with MMP-2 protein expression in ameloblastoma (r = -0.431, P < 0.01). Low or no RECK expression and increased MMP-2 expression may be associated with negative clinical findings in ameloblastoma. RECK may participate in the invasion, recurrence and malignant transformation of ameloblastoma by regulating MMP-2 at the post

  13. Imaging diagnosis of ameloblastoma; Bildgebung bei Ameloblastomen

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    Esser, M.; Horger, M.; Ioanovicu, S.D.; Boesmueller, H.

    2015-10-15

    Ameloblastomas are ondontogenic tumors of the upper and lower jaw. The ameloblastomas are supposed to be benign with slow growth, but locally invasive growth is possible. Contrast agent enhanced CT seems to have the largest imaging potential to differentiate between benign and malign osteogenic processes. In general - dependent on localization, histological type and neighboring tissues - the radical resection with following plastic reconstruction seems to be the best therapeutic decision.

  14. Calretinin as a Diagnostic Adjunct for Ameloblastoma

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    Chitra Anandani

    2014-01-01

    Full Text Available Background. Calretinin is a 29 kDa calcium-binding protein of the EF-hand family which is expressed in a variety of normal and tumorigenic tissues. Its expression in odontogenic epithelium during odontogenesis and in neoplastic odontogenic tissues has been demonstrated. Unicystic ameloblastoma poses a diagnostic challenge, as its histologic presentation can be sometimes mistaken for keratocystic odontogenic tumor (KCOT. This study was performed to assess the usefulness of calretinin as a confirmatory marker for ameloblastic tissue. Methodology. Total of 40 cases: 16 unicystic ameloblastoma, 4 multicystic ameloblastoma, and 20 KCOT, were evaluated immunohistochemically for the presence, localization, distribution, and intensity of calretinin expression. Statistical analysis was done using Chi-square test to intercompare the expression between ameloblastoma and KCOT. Results. Sixteen cases of ameloblastoma (12 unicystic, 4 multicystic showed positive calretinin staining of ameloblastic epithelium and only one case of KCOT was positive for calretinin, with the positivity restricted to the stellate reticulum like epithelium. Intercomparison between two groups revealed statistically significant difference (P=0.000. Conclusion. Calretinin appears to be a specific immunohistochemical marker for neoplastic ameloblastic epithelium and may be an important diagnostic adjunct in the differential diagnosis of ameloblastoma and KCOT.

  15. Radiotherapy in the treatment of ameloblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Gardner, D.G.

    1988-01-01

    There is a lack of well-documented evidence in the literature concerning the relative radioresponsiveness or radioresistance of ameloblastomas, although they are generally considered radioresistant. Most of the previous papers reffering to this subject lack acceptable histopathologic proof of the diagnosis, adequate radiotherapeutic data, and/or follow-up information; many predate the use of megavoltage irradiation. The present article reports 5 cases of ameloblastoma that were treated by radiotherapy, 3 by megavoltage irradiation. All have been confirmed histologically by an oral pathologist, all have adequate radiotherapeutic data and, with one exception, adequate follow-up information. In addition, the reasons for our present lack of knowledge of this subject are discussed; objective criteria are presented for evaluating the results of radiotherapy on ameloblastomas; the only other series of ameloblastomas treated by megavoltage irradition is analyzed. Although radiotherapy can reduce the size of an ameloblastoma, primarily that part of the tumor which has expanded the jaw or broken into the soft tissues, it does not appear to be an appropriate treatment for an operable ameloblastoma. Its main use is in inoperable cases, primarily in the posterior maxilla.

  16. The variability and complexity of ameloblastoma: carcinoma ex ameloblastoma or primary ameloblastic carcinoma.

    Science.gov (United States)

    Lin, Zitong; Chen, Fei; Wang, Tiemei; Hu, Qingang; Sun, Guowen

    2013-04-01

    Ameloblastoma is characterized by slow-growing, local invasiveness and high incidence of local recurrence. It usually presents with a benign histological appearance. However, ameloblastoma occasionally demonstrates a clinical course that is characteristic of malignant transformation. Here, we present a case of ameloblastoma with an aggressive clinical course, including multiple recurrences, a short disease-free interval, pulmonary metastasis and extensive skull-base infiltration. With a careful re-evaluation of the histology and cytology of the specimens of primary and recurrent ameloblastoma in 2006 and 2007, malignant transformation was observed and carcinoma ex ameloblastoma was ultimately diagnosed. Copyright © 2012 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

  17. Basal Cell Ameloblastoma: A Rare Histological Variant of an ...

    African Journals Online (AJOL)

    Ameloblastomas are an inscrutable group of oral tumors. Basal cell ameloblastoma is a rare variant of ameloblastoma with very few cases reported until date. The tumor is composed of more primitive cells and has less conspicuous peripheral palisading. It shows remarkable similarity to basal cell carcinoma, basal cell ...

  18. Maxillary unicystic ameloblastoma: a case report.

    Science.gov (United States)

    Agani, Zana; Hamiti-Krasniqi, Vjosa; Recica, Jehona; Loxha, Mergime Prekazi; Kurshumliu, Fisnik; Rexhepi, Aida

    2016-10-18

    Ameloblastoma is a benign epithelial odontogenic tumor. It is often aggressive and destructive, with the capacity to attain great size, erode bone and invade adjacent structures. Unicystic ameloblastoma is a rare odontogenic lesion, with clinical, radiographic and gross features of jaw cysts. The lesion histologically shows typical ameloblastomatous epithelium lining part of the cyst cavity with or without and/or mural tumor growth. Unicystic ameloblastoma usually presents in posterior mandibular ramus region, while it is rare and atypical in posterior maxillary region. . We report a case of 16 year old Kosovar male, Albanian ethnicity, who presented with a swelling located in right maxillary region. Clinical examination revealed a painless swelling extending from the maxillary right central incisor to the maxillary right first molar tooth. Panoramic radiograph disclosed a well corticated unilocular radiolucent lesion approximately 5 × 5 cm in diameter which was in contact with the roots of the teeth present inferiorly and with the maxillary sinus superiorly. Maxillary right canine impaction was noted and unerupted lateral incisor tooth was present inside the radiolucency. Preoperative diagnosis of the lesion was made as dentigerous cyst based on the age of the patient, location of the swelling, clinical and radiographic findings, but the unicystic ameloblastoma was also taken into consideration. The patient was treated by surgical enucleation of the lesion and extraction of lateral incisor tooth which was present inside the lesion. The histopathological examination of the lesion revealed confirmed finding for unicystic ameloblastoma mural form. No recurrence was observed in 1 year follow-up. Maxillary region is considered a rare and atypical location for unicystic ameloblastoma. We emphasize the importance of differential diagnosis of an odontogenic lesion with common clinical and radiological features that will impact the treatment planning and follow up

  19. Primary Ameloblastoma of the Temporal Bone.

    Science.gov (United States)

    Košec, Andro; Ajduk, Jakov; Ries, Mihael; Trotić, Robert

    2017-06-01

    Ameloblastoma is a locally aggressive tumor derived from odontogenic epithelium. Although benign, its clinical behavior can often exhibit malignant characteristics. It is marked by slow and persistent growth with infiltration of adjacent tissues. Almost 70% occur in the mandible in patients older than 30 years. Recurrence of ameloblastoma from inadequate treatment is frequent. Because of its slow growth, recurrences can present decades after primary surgery. A primary ameloblastoma in an area outside the mandibular, maxillary, and infratemporal fossa regions has not been described in detail to date, with only 1 possible case mentioned in the literature. The authors present a case of primary temporal bone ameloblastoma in a 17-year-old boy. The tumor originated in the left mastoid, infiltrated the lateral semicircular canal, facial nerve, and cochlea, and adhered to the sigmoid sinus and posterior cranial fossa dura. Although invasion of multiple structures in the infratemporal fossa and temporal bone leads to variable disease presentation, this case is unique because the first symptom of disease was sudden and recurring unilateral sensorineural hearing loss. Surgery required transection of the facial nerve. Histopathology confirmed primary temporal bone ameloblastoma. The difficulties in achieving wide surgical margins, diagnostics, and further management are addressed. Copyright © 2017 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  20. The expression profiles of acidic epithelial keratins in ameloblastoma.

    Science.gov (United States)

    Pal, Samir Kumar; Sakamoto, Kei; Aragaki, Tadanobu; Akashi, Takumi; Yamaguchi, Akira

    2013-04-01

    To characterize the subtypes of ameloblastoma by differentiation markers. Expression of 9 major acidic epithelial keratins was immunohistochemically examined in 28 ameloblastomas. Keratin 15 (K15) expression patterns corresponded to histological variants: follicular, plexiform and acanthomatous. Tumor nests comprising K15-expressing basal cells mimicked oral epithelium or dental lamina, and tumor nests comprising K15-negative basal cells mimicked outer enamel epithelium. Keratin 19 (K19) was consistently expressed in solid/multicystic ameloblastoma and unicystic ameloblastoma, while peripheral ameloblastoma and desmoplastic ameloblastoma contained K19-negative cells. The 4 current subtypes had unvaried expression patterns within each group. However, they could be divided into 2 groups by K19 expression pattern: solid/multicystic and unicystic versus extraosseous/peripheral and desmoplastic. K15 expression pattern represented various types of differentiation for tumor nests mimicking tooth germ and oral epithelium. The results clarify the homogeneity and heterogeneity of ameloblastoma cell lineage and differentiation. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. Nonexpansile Unicystic Ameloblastoma: A Rare Case Report

    Directory of Open Access Journals (Sweden)

    Supreet Jain

    2017-01-01

    Full Text Available Ameloblastoma is the most common benign odontogenic tumor that has the potential to grow into a large size. Unicystic ameloblastoma (UA is a variant of the solid or multicystic ameloblastoma. It is a rare (relative frequency 5–22% benign, locally invasive odontogenic tumor of young age that mimics clinically and radiographically as an odontogenic cyst. Radiographically it may present as unilocualr or multilocular with cortical plate expansion. Conventional radiograph has less advantage to determine their extension into soft tissue. Hence, conventional tomography, cone-beam computed tomography, and magnetic resonance imaging may be used to determine expansion, root resorption and relation with adjacent structures. It has low recurrence rate after conservative therapy. Here, we present a rare case of nonexpansile UA with an unusual presentation in a young male patient.

  2. A Conservative Approach to a Peripheral Ameloblastoma

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    Rocco Borrello

    2016-01-01

    Full Text Available Peripheral Ameloblastoma (PA is the rarest variant of ameloblastoma. It differs from the other subtypes of ameloblastoma in its localization: it arises in the soft tissues of the oral cavity coating the tooth bearing bones. Generally, it manifests nonaggressive behavior and it can be treated with complete removal by local conservative excision. In this study we report a case of PA of the maxilla in a 78-year-old female patient and we describe the four different histopathological patterns revealed by histological examination. After local excision and diagnosis, we planned a long term follow-up: in one year no recurrence had been reported. The choice of treatment is illustrated in Discussion.

  3. Ameloblastoma: Our clinical experience with 68 cases

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    Benjamin Fomete

    2014-01-01

    Full Text Available Introduction: In this environment, previous workers have reported on the challenges of managing large sized ameloblastoma of the jaws with less than adequate facilities. The aim of this review is to present the management of 68 cases of ameloblastoma with emphasis on surgical care. Materials and Methods: Retrospective survey of case notes of patients with histopathologic diagnosis of ameloblastoma (using the criteria of Barnes et al., 2005 seen between January 2006 and August 2010 at the Maxillofacial Unit, Ahmadu Bello University Teaching Hospital, Shika-Zaria, Nigeria was undertaken. Data collected includes histopathological diagnosis, age, gender, clinical information on site of lesion, form of intubation and surgical procedure performed. Results: Out of 94 patients, 68 with histological diagnosis of ameloblastoma (59 mandibular and 9 maxillary were operated within the study period. The remainder (26 was not treated in hospital. Among 68 patients treated, more were males (38 than females (30, giving a male to female ratio of 1.3:1. The age range was between 14 and 74 years (mean-standard deviation. The duration of the symptoms ranged from 7 months to 24 years, most were follicular ameloblastoma (n = 13 followed by acanthomatous type (n = 7. Endotracheal intubation was the most common (n = 55 followed by fiber optic laryngoscopy (n = 8. The surgical approach most used was extended Risdon with intraoral (n = 24 followed by extended Risdon with lip split and intraoral (n = 17. Segmental resection (en block formed the bulk of our procedures (n = 22 followed by subtotal mandibulectomy (n = 16. Conclusion: The treatment of ameloblastoma remains controversial. Its destructive nature has left patients with wide defects difficult to reconstruct.

  4. Images of ameloblastoma: from radiography to tomography

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    Raúl Herrera-Mujica

    2015-07-01

    Full Text Available Odontogenic tumors are a group of lesions originated from odontogenesis course alterations. Within odontogenic tumors is ameloblastoma, its treatment probably is one of the most controversial, because its aggressive behavior, high recurrence and probably low malignancies. Radiographs play an important role in assessment from this tumor, but the limitation is they are in two dimensions exams. Cone beam computed tomography is a valuable tool because enable a comprehensive assessment of anatomical structures by multiple sections of the area. Therefore, evaluation of ameloblastoma is complete by cone beam computed tomography because bone structures do not show distortion neither magnification.

  5. Unusual imaging appearance of unicystic ameloblastoma

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    Lakshmi Kavitha Nadendla

    2012-01-01

    Full Text Available Unicystic ameloblastoma (UA refers to those cystic lesions that show clinical, radiographic, or gross features of a mandibular cyst, but on histologic examination show a typical ameloblastous epithelium lining part of the cystic cavity, with or without luminal and/or mural tumor growth. It accounts for 5-15% of all intraosseous ameloblastomas. We report a case of UA in a 35-year-old female with an unusual large multilocular (tennis racket appearance on the right body of mandible and illustrate the importance and complexity of differential diagnosis with a brief review of recent literature.

  6. Radiographic analysis of ameloblastoma: A retrospective study

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    Chandramani More

    2012-01-01

    Conclusion: Radiographs are an important aid for the diagnosis of oral lesions of various types, especially those that involve bone. It is important for the practicing clinicians to know the salient features of ameloblastoma which are peculiar to the local population.

  7. Rehabilitation of recurrent unicystic ameloblastoma using distraction ...

    African Journals Online (AJOL)

    This method of providing additional bone and soft tissue for implant placement is becoming more common. This clinical report describes the use of distraction osteogenesis and fixed implant supported prosthesis to treat a postsurgical alveolar defect as a result of the resection of a unicystic ameloblastoma in the anterior ...

  8. Premolar Cystic Ameloblastoma in a Child.

    Science.gov (United States)

    Pathak, Sanyog; Sonalika, Wanjari Ghate; Hs, Vanishree; Tegginammani, Anand S

    2017-01-01

    Mandibular swellings may occur as a result of many benign lesions of odontogenic or non-odontogenic origin. Ameloblastomas are benign tumours of odontogenic origin, whose importance lies in its potential to grow into enormous size with resulting bone deformity, it is a slow-growing, persistent, and locally aggressive neoplasm. The unicystic ameloblastoma (UA) represents an ameloblastoma variant, presenting as a cyst clinically and radiographically, but showing typical ameloblastomatous epithelium lining histologically. It commonly occurs in second and third decades of life and is rare in children under 12 years of age, and better response to conservative treatment. It shares many clinical and radiographic features with odontogenic cysts/tumours and/or periapical disease of endodontic origin. Reported here is an unusual case of unicystic ameloblastoma involving the crown of an unerupted mandibular first premolar in a 9-year boy in an uncommon location, which was misdiagnosed as periapical lesion of inflammatory origin clinically, and as a dentigerous cyst radiographically. This highlights the importance to routinely submit the removed surgical specimen for histopathological examination.

  9. Ameloblastoma Phenotypes Reflected in Distinct Transcriptome Profiles

    Science.gov (United States)

    Hu, Shijia; Parker, Joel; Divaris, Kimon; Padilla, Ricardo; Murrah, Valerie; Wright, John Timothy

    2016-01-01

    Ameloblastoma is a locally invasive benign neoplasm derived from odontogenic epithelium and presents with diverse phenotypes yet to be characterized molecularly. High recurrence rates of 50–80% with conservative treatment in some sub-types warrants radical surgical resections resulting in high morbidity. The objective of the study was to characterize the transcriptome of ameloblastoma and identify relevant genes and molecular pathways using normal odontogenic tissue (human “dentome”) for comparison. Laser capture microdissection was used to obtain neoplastic epithelial tissue from 17 tumors which were examined using the Agilent 44 k whole genome microarray. Ameloblastoma separated into 2 distinct molecular clusters that were associated with pre-secretory ameloblast and odontoblast. Within the pre-secretory cluster, 9/10 of samples were of the follicular type while 6/7 of the samples in the odontoblast cluster were of the plexiform type (p ameloblastoma sub-types and have implications for the use of tailored treatment. PMID:27491308

  10. Difficulties in the diagnosis of plexiform ameloblastoma.

    Science.gov (United States)

    Castro-Silva, Igor Iuco; Israel, Monica Simoes; Lima, Glauco Siqueira; de Queiroz Chaves Lourenço, Simone

    2012-03-01

    Plexiform ameloblastoma is a rare and benign odontogenic tumor which may reach grotesque proportions affecting over a region of lower molars. The correct diagnosis is essential to reduce this risk of local recurrences and obtain an effective treatment. A 10-year-old female patient, Caucasian, presented a complaint of painless facial asymmetry. The clinical and imaginological examinations showed an increase in volume in the left mandibular body related to radiolucent unilocular injury near the apex of the first molar, expansion of the vestibular cortical bone, and tooth root resorption ipsilaterally. The former intraosseous biopsy suggested an aneurysmal bone cyst. In reopening the previous marsupialization, a new biopsy was performed with histopathologic findings compatible with plexiform ameloblastoma associated with acute inflammation. Curettage was performed in the region associated with cryotherapy. At the last biopsy, the diagnosis of plexiform ameloblastoma was confirmed with multiple cystic formations and areas of foreign body reaction. The patient is being followed for 2 years and yet remains clinically and radiographically stable with no recurrence. This case reports the importance of selecting the correct area of biopsy of an intraosseous extended lesion to contribute to the diagnosis and treatment of plexiform ameloblastoma.

  11. Ameloblastoma during pregnancy: a case report.

    Science.gov (United States)

    da Silva, Helbert Eustáquio Cardoso; Costa, Erika do Socorro Ramos; Medeiros, Antônio Carlos Quintão; Pereira, Paulo Sérgio Dos Santos

    2016-09-06

    Ameloblastomas are rarely found in pregnant women, with only two cases reported in the scientific literature. We report the first case of ameloblastoma in a pregnant woman in Brazil. A 27-year-old white woman, 12-weeks pregnant, presented with a large mass in her right posterior mandible. Panoramic radiography revealed a lesion involving her mandibular right first molar with displacement of her mandibular right third molar and impairment of the mandibular bone base. The results of an incisional biopsy led to a diagnosis of acanthomatous ameloblastoma. We fixed Erich arch bars to both dental arches and performed an en-bloc resection surgery under general anesthesia for tumor removal. She was then treated by maxillomandibular rigid fixation with the installation of a 2.7 mm non-locking reconstruction plate. So far, she has presented no motor deficits, chewing difficulties, or relevant asymmetries. The tumor showed no recurrence after the first year (pregnancy period) and post-surgery radiographic follow-up revealed a reduction in the surgical area after osseous growth in the margins of the lesion. Although she displayed no systemic comorbidities that affected pregnancy, the fetus was born with alobar holoprosencephaly. The possible influence of pregnancy hormones on the growth and development of tumors in general and ameloblastoma in particular, is still not explained in the literature. However, evidence reveals that the issue should be further studied. Although en-bloc resection surgery is considered a radical method of treatment, it is an effective alternative in ameloblastoma removal, presenting low rates of recurrence.

  12. Unicystic ameloblastoma with mural proliferation: conservative or surgical approach?

    Science.gov (United States)

    Samuel, Soumi; Mistry, Freddy K; Chopra, Shilpa; Pillai, Ajay

    2014-01-01

    Ameloblastoma occurs in a wide variety of forms. Various forms of ameloblastomas have various treatment modalities ranging from a conservative approach to surgical resection with reconstruction. We report a case of unicystic ameloblastoma with mural proliferation in a 17-year-old girl, who presented with a swelling in the lower left jaw associated with dull aching pain and was managed initially by a conservative approach followed by surgical enucleation on recurrence. PMID:25103487

  13. Unicystic Ameloblastoma with Mural Proliferation Managed by Conservative Treatment

    Directory of Open Access Journals (Sweden)

    Natália Galvão Garcia

    2016-01-01

    Full Text Available Unicystic ameloblastoma is a distinguishable entity of ameloblastomas, characterized by slow growth and being relatively locally aggressive. Three histological types are recognized according to the degree of ameloblastomatous epithelial extension, namely, luminal, intraluminal, and mural types. This classification has a direct bearing on their biological behavior, treatment, and prognosis. However, there is difficulty in determining the most appropriate form of treatment for unicystic ameloblastoma. We present a case of unicystic ameloblastoma that occurred in the right posterior mandible of 19-year-old girl, which was enucleated and did not recur after 12-month follow-up.

  14. Recurrent mandibular ameloblastoma with anterior skull base invasion: Case report.

    Science.gov (United States)

    Santini, L; Varoquaux, A; Giovanni, A; Dessi, P; Michel, J

    2015-01-01

    Recurrent ameloblastoma with skull base invasion is a rare clinical entity with poor prognosis. We report a case of a mandibular ameloblastoma recurrence involving the anterior skull base. The diagnostic and therapeutic processes are presented with emphasis on the radiologic features of ameloblastoma. Another aim of this case report is to underline the importance of close and long-term follow-up after resection. Ameloblastoma recurrences are frequent and mainly occur after incomplete surgical resection. These recurrences may be diagnosed late because of lack of symptoms in the mandibular area.

  15. Vascularized mandibular anterior ameloblastoma - an entity still unresolved.

    Science.gov (United States)

    Maheshwari, P; Chandrashekhar, C; Radhakrishnan, R

    2013-05-01

    Vascularized ameloblastoma is a bewildering entity whose existence is questionable from its origin to nosology and its very characterization as a distinct variant of ameloblastoma. This uncertainty is largely because of a fewer number of documented cases and loss of long-term follow-up. The current paper describes two cases of ameloblastoma in the mandibular anterior region, which had features of so-called "hemangiomatous ameloblastoma" as it was originally described. Understanding its pathophysiology based on various views and clinical implications in terms of its biologic behavior are brought to light in this paper.

  16. Central Ameloblastoma with a Peripheral Ameloblastoma-Like Component: A Case Report

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    Seyyed Hosein Tabatabaei

    2014-09-01

    Full Text Available Amebloblastoma as the most common epithelial odontogenic neoplasm may occur in two forms of central and peripheral. This report presents a case of a 41-year-old Iranian female with a six-month complaint from a painless mass in the right posterior portion of the mandible. The case was diagnosed as an exophytic epulis-like peripheral component with characteristics of peripheral ameloblastoma and an intrabony component like solid ameloblastoma. Two probable hypotheses considered for this case is also discussed.

  17. Basal Cell Ameloblastoma: A Rare Histological Variant of an ...

    African Journals Online (AJOL)

    of ameloblastoma.[4] The microscopic features of basal cell ameloblastoma, however, are similar to those of several malignant tumors, including basaloid squamous cell carcinoma (BSCC),[5,6] cutaneous basal cell carcinoma (BCC) and solid‑type adenoid cystic carcinoma (ACC).[1] The pathologist may sometimes fail to.

  18. Ameloblastoma in Tanzania: A retrospective analysis of histological ...

    African Journals Online (AJOL)

    World-wide, ameloblastoma have been discussed in relation and modalities of treatment. In Tanzania, case,s of ameloblastoma are seen but no data is available to quantify the magnitude of the disease within the community. The present study aims at describing the occurrence, sex, age and regional distribution of ...

  19. case reports treatment of ameloblastoma of the jaws in children

    African Journals Online (AJOL)

    2011-03-01

    Mar 1, 2011 ... SUMMARY. Background: To report the surgical experience on the treatment of ameloblastoma of the jaws in children. Method: This is a prospective study of six children below the age of 16 years with ameloblastoma of the jaws seen at the Maxillofacial Unit of the Department of Surgery, Korle Bu Teaching ...

  20. Treatment of ameloblastoma of the jaws in children | Abdulai ...

    African Journals Online (AJOL)

    Background: To report the surgical experience on the treatment of ameloblastoma of the jaws in children. Method: This is a prospective study of six children below the age of 16 years with ameloblastoma of the jaws seen at the Maxillofacial Unit of the Department of Surgery, Korle Bu Teaching Hospital over a fifteen

  1. Maxillary Ameloblastoma: An Enigma for the Surgeon. | Ajike ...

    African Journals Online (AJOL)

    Background: Ameloblastoma is a benign but locally aggressive odontogenic tumour. Worldwide, maxillary ameloblastoma is rare but its late detection renders adequate treatment difficult. Majority occur in the mandible with about 5-20% occurring in the maxillary bone. Objective: The purpose of this study was to analyze 21 ...

  2. The Complexity of Stromal Changes in Desmoplastic Ameloblastoma

    African Journals Online (AJOL)

    Ameloblastoma is usually considered a homogenous neoplasm and is thought of as the most primitive of all odontogenic neoplasms. However, detailed investigations have proven clinicopathological diversity in a significant number of cases, thus mounting the evidence in favor of considering ameloblastoma as a ...

  3. Desmoplastic ameloblastoma of anterior mandible: Case report of a rarity

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    Prashant Nahar

    2008-01-01

    Full Text Available Ameloblastoma, one of the most common odontogenic tumors of the jaws, presents classical clinical, radiographic and histopathological diagnostic features exhibiting a benign but locally aggressive and destructive clinical course with a high rate of recurrence. A case of desmoplastic ameloblastoma of mandible is discussed in this article, which presents itself as a rare variant of ameloblastoma with unusual inconclusive clinico-radiographic features to be diagnosed as classical ameloblastoma and difficult to differentiate from other suspected multilocular benign odontogenic or reactive lesions of the jaws. It is the typical histopathological picture of the lesion exhibiting a blend of desmoplasia (collagenization and ameloblastoma that leads to its final diagnosis and determines its management.

  4. Unicystic ameloblastoma of the maxilla: A case report

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    Paikkatt V

    2007-06-01

    Full Text Available Unicystic ameloblastoma is believed to be less aggressive and responds more favorably to conservative surgery than the solid or multicystic ameloblastomas. This report is a rare case of unicystic ameloblastoma of the maxilla that was treated by enucleation under suspicion of a radicular cyst related to a dens in dente. The neoplastic nature of the lesion became evident only when the enucleated material was available for histologic examination. With this report, the authors illustrate the importance and complexity of a differential diagnosis of lesions with a cystic aspect in the anterior region of the maxilla, among them - inflammatory radicular cysts, odontogenic keratocysts, adenomatoid odontogenic and unicystic ameloblastoma. Relevant diagnostic problems and choice of treatment of unicystic ameloblastoma are presented along with a review of the literature.

  5. Malignant ameloblastoma metastasis to the neck: Radiological and pathohistological dilemma

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    Golubović Mileta

    2012-01-01

    Full Text Available Introduction. Ameloblastomas are odontogenic epithelial, locally invasive tumors of slow growth and mostly of benign behavior. Their frequency is low (they account for 1% of all head and neck tumors and about 11% of tumors of dental origin. Malignant variations of ameloblastoma are malignant ameloblastoma and ameloblastic carcinoma. They constitute less than 1% of all ameloblastomas. We presented a case of malignant ameloblastoma of the mandible with neck metastasis. Case report. A patient, aged 72, presented with the following symptoms: pain in the lower jaw, swelling in the left submandibular area and difficult mouth opening. The patient was admitted to the Department of Oral and Maxillofacial Surgery, Clinical Center of Montenegro, two months after he had noticed the symptoms. Panoramic radiography (OPG showed that both jaws were partially toothless with terminal stage of periodontitis of the remaining teeth. Also, OPG showed sharply limited semicircular defect in the retromolar region and along the front edge of the mandible rami. Conventional histopathologic examination of the neck masses showed malignant ameloblastoma which contained central fields of squamous differentiation. Immunoreactivity of several markers was determined using immunohistochemical analyses. After these diagnostic methods a definite histopathology diagnosis was made: Ameloblastoma metastaticum in textus fibroadiposus regio colli (typus acanthomatosus. Conclusion. It is not possible to distinguish conventional, ie intraosseous, ameloblastoma from malignant ameloblastoma according to histopathologic features. It is necessary to pay special attention, especially in elderly patients, and to carry out further clinical, radiological and pathohistological diagnostic procedures, such as immunohistochemical analysis. A timely and correct diagnosis and treatment of malignant ameloblastoma require a multidisciplinary approach.

  6. XRCC1 gene polymorphisms and risk of ameloblastoma.

    Science.gov (United States)

    Yanatatsaneejit, Pattamawadee; Boonsuwan, Titiporn; Mutirangura, Apiwat; Kitkumthorn, Nakarin

    2013-06-01

    Ameloblastoma is a common benign odontogenic tumour with inherently aggressive behaviour. Genetic susceptibility of single nucleotide polymorphism (SNP) can likely predict ameloblastoma at risk patients but this data remains limited. Here, we studied XRCC1 polymorphism as a risk factor for ameloblastoma. Eighty-two ameloblastoma samples and blood from 140 healthy controls were used to perform polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for XRCC1 at codons 194, 280 and 399, and confirmed by sequence analysis. Compare to healthy control, a significant increase was noted in the occurrence of polymorphism at codon 194 and 399 in ameloblastoma patients. At codon 194, tryptophan encoded by T, was the susceptibility allele showed an ODD ratio of (95% CI)=1.62 (1.05-2.48), p=0.027. At codon 399, glycine encoded by A was the susceptibility allele showing ODD ratio of (95% CI)=1.83 (1.19-2.84), p=0.005. Moreover at codon 399, we found AG as the susceptibility genotype (2.06 (1.14-3.72), p=0.015). However, we did not find any significant increase in polymorphic occurrence in ameloblastoma patients at codon 280. For haplotype analysis of 3 codons, we found GGC as protective haplotype, and AGT as the risk haplotype. Our data suggest that polymorphism at codons 194 and 399, likely contributes to the risk of developing ameloblastoma. Copyright © 2012 Elsevier Ltd. All rights reserved.

  7. Retrospective study of ameloblastoma: the possibility of conservative treatment.

    Science.gov (United States)

    Hasegawa, Takumi; Imai, Yusuke; Takeda, Daisuke; Yasuoka, Daisuke; Ri, Shinshou; Shigeta, Takashi; Minamikawa, Tsutomu; Shibuya, Yasuyuki; Komori, Takahide

    2013-11-09

    At our institutions, most cases of the solid or multicystic type were treated as conservatively as possible in order to avoid disadvantages of radical treatment. The aim of present study was to retrospectively analyze the ameloblastoma cases diagnosed at our two institutions, to classify them according to the criteria of the 2005 WHO classification, and to evaluate the possibility of using a conservative approach for the surgical treatment of ameloblastoma. Maxillary cases, unicystic cases, peripheral cases and resection-treated cases were excluded from this study. In 23 tumors of mandibular solid or multicystic ameloblastoma, a patient's age, gender, location, clinical signs, duration, radiographic appearance, preoperative diagnosis, ameloblastoma subtypes, treatment, and recurrence were investigated. The recurrence rate (48.7%) in this study was lower than the reported recurrence rate after conservative treatment for solid or multicystic ameloblastoma and was higher than the reported recurrence rate of ameloblastoma, inclusive of other types. However, all patients who were diagnosed with recurrences have maintained their quality of life and were satisfied for at least several years after the conservative treatment. In conclusion, we demonstrated one possibility that a conservative approach might be employed in the surgical treatment of ameloblastoma (even of the solid or multicystic type).

  8. Hemimandibulectomy with full angular mandibular plate reconstruction follicular Ameloblastoma patient

    Directory of Open Access Journals (Sweden)

    Susanti Bulan

    2017-08-01

    Full Text Available Introduction :Ameloblastoma is an aggressive benign of odontogenic origin tumor; cystic shape, slowly growth; no pain; local invasive; infiltrate muscle; and bone destruction. Based on histopathology ameloblastomas are classified: Follicular, acanthomatous, granular cell, basal cell, and plexiform. Follicular and plexiformameloblastomas are the most common, with incidence rates 27.7% and 21.1%. Follicular ameloblastoma is characterized by recurrence rate (29.5%; plexiformameloblastoma (16.7%; and acanthomatousameloblastoma (4.5%. Early diagnosis with prompt and adequate management decrease recurrence and get good prognosis. Objective : to evaluate recurrence of follicular ameloblastoma after surgery; to evaluate  stomatognatic function and aesthetic problems. Case report : a 55 y.o. female patient with lump at left cheek since 1 year before admission; initially was small in size; gradually increased, no history of trauma, pain, febrile, and discharge. Based on clinical, histopathological, radiological, CT scan, it was diagnosed follicular ameloblastoma. The treatment was left hemimandibulectomy with full angular mandibular plate reconstruction. Discussion :Ameloblastoma is a locally destructive tumor with recurrence if not entirely excised. The goal of treatment ameloblastoma is wide excision and reconstruction of surgical defect. Then, it is followed up to evaluate recurrence, stomatognatic function, and aesthetic problems. Conclusion : Prognosis is good if an early diagnosis of the lesion is made with prompt and adequate surgical intervention.

  9. High frequency of BRAF V600E mutations in ameloblastoma.

    Science.gov (United States)

    Kurppa, Kari J; Catón, Javier; Morgan, Peter R; Ristimäki, Ari; Ruhin, Blandine; Kellokoski, Jari; Elenius, Klaus; Heikinheimo, Kristiina

    2014-04-01

    Ameloblastoma is a benign but locally infiltrative odontogenic neoplasm. Although ameloblastomas rarely metastasise, recurrences together with radical surgery often result in facial deformity and significant morbidity. Development of non-invasive therapies has been precluded by a lack of understanding of the molecular background of ameloblastoma pathogenesis. When addressing the role of ERBB receptors as potential new targets for ameloblastoma, we discovered significant EGFR over-expression in clinical samples using real-time RT-PCR, but observed variable sensitivity of novel primary ameloblastoma cells to EGFR-targeted drugs in vitro. In the quest for mutations downstream of EGFR that could explain this apparent discrepancy, Sanger sequencing revealed an oncogenic BRAF V600E mutation in the cell line resistant to EGFR inhibition. Further analysis of the clinical samples by Sanger sequencing and BRAF V600E-specific immunohistochemistry demonstrated a high frequency of BRAF V600E mutations (15 of 24 samples, 63%). These data provide novel insight into the poorly understood molecular pathogenesis of ameloblastoma and offer a rationale to test drugs targeting EGFR or mutant BRAF as novel therapies for ameloblastoma. © 2013 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

  10. Mandible ameloblastoma with lung metastasis: a rare case report.

    Science.gov (United States)

    Yang, Rui-Na; Wang, Xin-Shuai; Ren, Jing; Xie, Yan-Fei; Zhou, Dan; Ge, Dong-Feng; Feng, Xiao-Shan; Gao, She-Gan

    2015-01-01

    The ameloblastoma is the most common odontogenic epithelial tumor, which belong to benign neoplasms that present a painless course, and usually occur in the oromaxillo-facial region. Although the histopathological manifestation of ameloblastoma is benign, it has unique biological behavior, for example local invasion and recurrence repeatedly. A few case of ameloblastoma was locally aggressive growth, and rarely metastasis to other tissue, for example the lungs, lymph nodes, and spine. A 64-year-old Chinese man, diagnosed with metastatic ameloblastoma, was treated with palliative chemotherapy consisting of cyclophosphamide, doxorubicin, and cisplatin for six cycles, and radiotherapy for 50 Gy after the last cycle chemotherapy. During the surveillance CT scan after the therapy, the tissues of the tumor were nearly complete response. The purpose of this study was to report a case of a patient with a right mandible ameloblastoma that recurred repeatedly and metastasized into bilateral lung. After the chemotherapy and radiotherapy, the tissues of the tumor were nearly complete response. This case is interesting because it investigated the diagnosis and treatment of the malignancy ameloblastoma, as this may help diagnose and treatment for clinician to the metastatic ameloblastoma.

  11. Activating FGFR2-RAS-BRAF mutations in ameloblastoma.

    Science.gov (United States)

    Brown, Noah A; Rolland, Delphine; McHugh, Jonathan B; Weigelin, Helmut C; Zhao, Lili; Lim, Megan S; Elenitoba-Johnson, Kojo S J; Betz, Bryan L

    2014-11-01

    Ameloblastoma is an odontogenic neoplasm whose overall mutational landscape has not been well characterized. We sought to characterize pathogenic mutations in ameloblastoma and their clinical and functional significance with an emphasis on the mitogen-activated protein kinase (MAPK) pathway. A total of 84 ameloblastomas and 40 non-ameloblastoma odontogenic tumors were evaluated with a combination of BRAF V600E allele-specific PCR, VE1 immunohistochemistry, the Ion AmpliSeq Cancer Hotspot Panel, and Sanger sequencing. Efficacy of a BRAF inhibitor was evaluated in an ameloblastoma-derived cell line. Somatic, activating, and mutually exclusive RAS-BRAF and FGFR2 mutations were identified in 88% of cases. Somatic mutations in SMO, CTNNB1, PIK3CA, and SMARCB1 were also identified. BRAF V600E was the most common mutation, found in 62% of ameloblastomas and in ameloblastic fibromas/fibrodentinomas but not in other odontogenic tumors. This mutation was associated with a younger age of onset, whereas BRAF wild-type cases arose more frequently in the maxilla and showed earlier recurrences. One hundred percent concordance was observed between VE1 immunohistochemistry and molecular detection of BRAF V600E mutations. Ameloblastoma cells demonstrated constitutive MAPK pathway activation in vitro. Proliferation and MAPK activation were potently inhibited by the BRAF inhibitor vemurafenib. Our findings suggest that activating FGFR2-RAS-BRAF mutations play a critical role in the pathogenesis of most cases of ameloblastoma. Somatic mutations in SMO, CTNNB1, PIK3CA, and SMARCB1 may function as secondary mutations. BRAF V600E mutations have both diagnostic and prognostic implications. In vitro response of ameloblastoma to a BRAF inhibitor suggests a potential role for targeted therapy. ©2014 American Association for Cancer Research.

  12. Immunohistochemical detection of p53 protein in ameloblastoma types.

    Science.gov (United States)

    el-Sissy, N A

    1999-05-01

    Overexpression of p53 protein in unicystic ameloblastoma (uAB) is denser than in the conventional ameloblastoma (cAB) type, indicating increased wild type p53--suppressing the growth potential of uAB and denoting the early event of neoplastic transformation, probably of a previous odontogenic cyst. Overexpression of p53 in borderline cAB and malignant ameloblastoma (mAB) types might reflect a mutational p53 protein playing an oncogenic role, promoting tumour growth. Overexpression of p53 protein could be a valid screening method for predicting underlying malignant genetic changes in AB types, through increased frequency of immunoreactive cells or increased staining density.

  13. Ameloblastoma: A Review of Recent Molecular Pathogenetic Discoveries

    Directory of Open Access Journals (Sweden)

    Noah A. Brown

    2015-01-01

    Full Text Available Ameloblastoma is an odontogenic neoplasm whose molecular pathogenesis has only recently been elucidated. The discovery of recurrent activating mutations in FGFR2, BRAF , and RAS in a large majority of ameloblastomas has implicated dysregulation of MAPK pathway signaling as a critical step in the pathogenesis of this tumor. Some degree of controversy exists regarding the role of mutations affecting the sonic hedgehog (SHH pathway, specifically Smoothened (SMO, which have been postulated to serve as either an alternative pathogenetic mechanism or secondary mutations. Here, we review recent advances in our understanding of the molecular pathogenesis of ameloblastoma as well as the diagnostic, prognostic, and therapeutic implications of these discoveries.

  14. Recurrent unicystic ameloblastoma in mandibular anterior teeth area

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    Lee, Won Do; Lee, Wan; Kim, Jin Hoa; Choi, Dong Hoon; Paeng, Jun Young; Kim, Eun Cheol [Department of Oral and Maxillofacial Radiology, School of Dentistry, Wonkwang University, Wonkwang Dental Research Institute, Iksan (Korea, Republic of)

    2008-06-15

    The unicystic ameloblastoma (UA) is a variant of the solid or multicystic ameloblastoma, a less encountered variant of the ameloblastoma. It appears more frequently in the second or third decade with no sexual or racial predilection. It is almost exclusively encountered asymptomatically in the posterior mandible. We report a case of a 43-year old patient with UA, who had previously undergone a surgical treatment on the same site about 1 year ago, this lesion recurred and presented as an exophytic gingival lesion in the anterior mandibular region.

  15. Recurrent unicystic ameloblastoma in mandibular anterior teeth area

    International Nuclear Information System (INIS)

    Lee, Won Do; Lee, Wan; Kim, Jin Hoa; Choi, Dong Hoon; Paeng, Jun Young; Kim, Eun Cheol

    2008-01-01

    The unicystic ameloblastoma (UA) is a variant of the solid or multicystic ameloblastoma, a less encountered variant of the ameloblastoma. It appears more frequently in the second or third decade with no sexual or racial predilection. It is almost exclusively encountered asymptomatically in the posterior mandible. We report a case of a 43-year old patient with UA, who had previously undergone a surgical treatment on the same site about 1 year ago, this lesion recurred and presented as an exophytic gingival lesion in the anterior mandibular region.

  16. Unicystic plexiform ameloblastoma: An insight for pediatric dentists

    Directory of Open Access Journals (Sweden)

    Yavagal C

    2009-03-01

    Full Text Available Ameloblastomas have been categorized broadly into three biologic variants: cystic (unicystic, solid, and peripheral. The term plexiform unicystic ameloblastoma refers to a pattern of epithelial proliferation that has been described in cystic lesions of the jaws. Although the histology suggests that cystic ameloblastomas follow a biologically low-grade course, recent evidence suggests that they may often behave clinically as biologically aggressive tumors. This is supported by the high incidence of cortical perforation, tooth resorption, lesion size, bony destruction, and a high rate of recurrence after simple enucleation. This article tries to provide an insight for pediatric dentists regarding this biologically distinct entity. A literature review on the topic has been added along with a case report highlighting the state-of-the-art approach and management of such ameloblastomas, in pediatric patients.

  17. Molecular markers of cell adhesion in ameloblastomas. An update

    Science.gov (United States)

    González-González, Rogelio; Molina-Frechero, Nelly; Damian-Matsumura, Pablo

    2014-01-01

    Ameloblastoma is the most common odontogenic tumor of epithelial origin, and though it is of a benign nature, it frequently infiltrates the bone, has a high rate of recurrence and could potentially become malignant. Cellular adhesion potentially plays an important role in the manifestation of these characteristics and in the tumor biology of ameloblastomas. Losses of cell-cell and extracellular matrix adhesion and cohesion are among the first events that occur in the invasion and growth of tumors of epithelial origin. The present review includes a description of the molecules that are involved in cell adhesion as reported for various types of ameloblastomas and discusses the possible roles of these molecules in the biological behaviors of this odontogenic tumor. Knowledge of the complex mechanisms in which these molecules play a role is critical for the research and discovery of future therapeutic targets. Key words:Ameloblastoma, cellular adhesion, molecular markers, cell-cell adhesion, extracellular matrix-cell adhesion. PMID:23986011

  18. Massive granular cell ameloblastoma with dural extension and atypical morphology

    Directory of Open Access Journals (Sweden)

    Vandana Raghunath

    2014-01-01

    Full Text Available Ameloblastomas are rare histologically benign, locally aggressive tumors arising from the oral ectoderm that occasionally reach a gigantic size. Giant ameloblastomas are a rarity these days with the advent of panoramic radiography in routine dental practice. Furthermore, the granular cell variant is an uncommon histological subtype of ameloblastoma where the central stellate reticulum like cells in tumor follicles is replaced by granular cells. Although granular cell ameloblastoma (GCA is considered to be a destructive tumor with a high recurrence rate, the significance of granular cells in predicting its biologic behavior is debatable. However, we present a rare case of giant GCA of remarkable histomorphology showing extensive craniofacial involvement and dural extension that rendered a good prognosis following treatment.

  19. Radiotherapy for a repeatedly recurrent ameloblastoma with malignant transformation.

    Science.gov (United States)

    Huang, Chun-Ming; Chen, Jin-Yi; Chen, Chung-Ho; Huang, Chih-Jen

    2014-01-01

    The role of radiation therapy (RT) for ameloblastoma remains controversial and undetermined due to the rarity of the disease. A case of repeatedly recurrent ameloblastoma with malignant transformation is presented. The clinical course and managements are described. The 63-year-old man had a recurrent ameloblastoma in the left mandible. Five years after the first surgical resection, he underwent 8 more rounds of surgical excision of the recurrent tumors. The malignant transformation occurred and the unresectable tumor invaded the masticator space, parapharyngeal space, and skull base. He received 3-dimensional conformal RT, at the dose of 66 Gray (Gy) in 33 fractions. The ulcerative exophytic mass had regressed gradually. After follow-up of 28 months, the tumor was well controlled. RT seems to be a feasible treatment option for recurrent ameloblastoma with malignant transformation. Copyright © 2013 Wiley Periodicals, Inc.

  20. [Ameloblastoma of the maxillary sinus treated with radiation therapy].

    Science.gov (United States)

    Chehal, Asmaa; Lobo, Rosabel; Naim, Asmaa; Azinovic, Ignacio

    2017-01-01

    Ameloblastoma is a benign aggressive odontogenic tumor which requires early diagnosis and appropriate treatment. It commonly affects the mandible and radical surgery is the gold standard treatment. We report the case of a patient with ameloblastoma in extremely advanced phase affecting the maxillary sinus who was treated with intensity modulated conformal radiation therapy. Patient's evolution was marked by complete remission maintained after 24 months follow-up. Maxillary ameloblastoma is not well documented in the literature. It is usually diagnosed at the later stage when optimal surgery cannot be performed. This case study aimed to demonstrate that radiation therapy is a real therapeutic alternative in the treatment of advanced and inoperable forms of ameloblastoma.

  1. Endoscopic transnasal resection of ameloblastoma with intracranial extension.

    Science.gov (United States)

    Woodroffe, Royce W; Abel, Taylor J; Fletcher, Aaron; Grossbach, Andrew; Van Daele, Douglas J; O'Brien, Erin; Greenlee, Jeremy D W

    2014-05-01

    Ameloblastoma is a rare odontogenic tumor with characteristics of epithelial tissue that produces enamel for the developing tooth. This lesion is generally considered benign, but has malignant forms that invade locally and metastasize. We present a 60-year-old man with maxillary ameloblastoma that after multiple recurrences developed intracranial extension with dural involvement of the middle cranial fossa and was treated by endoscopic transnasal resection followed by radiation therapy. Our technique and intraoperative findings are described with a review of the literature on intracranial ameloblastoma. This patient represents a unique account of endoscopic transnasal resection being utilized in the treatment of intracranial extension of ameloblastoma and demonstrates potential for application in similar cases. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Hemimaxillectomy for desmoplastic ameloblastoma with immediate temporalis flap reconstruction.

    Science.gov (United States)

    Elo, Jeffrey A; Tandon, Rahul; Allen, Chad N; Murray, Matthew D

    2014-08-01

    Ameloblastoma is one of the most common odontogenic tumors encountered, occurring more frequently than all other tumors combined, if one excludes the keratocystic odontogenic tumor. These tumors can cause severe expansion of the cortical bones and gross anatomic deformities. They can affect the dentition, causing tooth mobility and displacement. Fortunately, morbidity can be minimized with recognition on routine radiographic examination. The tissue may be unilocular or multilocular and has been described as having a "soap-bubble" appearance. Nevertheless, its radiographic appearance is insufficient to make a definitive diagnosis, because other tumors have similar appearance. Although the anatomic distribution and progression of ameloblastoma remain fairly consistent, alternative manifestations follow an atypical clinical course. One such variant is the desmoplastic ameloblastoma. We present a case of maxillary desmoplastic ameloblastoma treated with hemimaxillectomy and immediate reconstruction with temporalis flap that was recurrence-free at 36 months. Copyright © 2014 Elsevier Inc. All rights reserved.

  3. RECK overexpression reduces invasive ability in ameloblastoma cells.

    Science.gov (United States)

    Liang, Qi-xiang; Liang, Yan-can; Xu, Zhi-ying; Chen, Wei-liang; Xie, Hong-liang; Zhang, Bin

    2014-09-01

    Ameloblastoma is a frequent odontogenic neoplasm characterized by local invasiveness and high risk of recurrence. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is a tumor suppressor that inhibits metastasis and angiogenesis. The aim of this study was to investigate effects of RECK overexpression on invasive potential in ameloblastoma cells. Lentiviral vectors containing human RECK gene were created and subsequently stably transfected into immortalized ameloblastoma cell line hTERT(+) -AM. Functional characteristics of hTERT(+) -AM cells with stable RECK overexpression included proliferation, migration, invasion, and regulation of matrix metalloproteinases (MMP)-2, MMP-9 measured by zymography or commercially available assays. The stable and higher expression of RECK mRNA and protein (P 0.05). Overexpression of RECK gene significantly inhibited cell invasive ability of hTERT(+) -AM cells, suggesting RECK may be a new target for ameloblastoma treatment. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  4. Ameloblastoma: a clinical review and trends in management.

    Science.gov (United States)

    McClary, Andrew C; West, Robert B; McClary, Ashley C; Pollack, Jonathan R; Fischbein, Nancy J; Holsinger, Christopher F; Sunwoo, John; Colevas, A Dimitrios; Sirjani, Davud

    2016-07-01

    Ameloblastoma is a rare odontogenic neoplasm of the mandible and maxilla, with multiple histologic variants, and high recurrence rates if improperly treated. The current mainstay of treatment is wide local excision with appropriate margins and immediate reconstruction. Here we review the ameloblastoma literature, using the available evidence to highlight the change in management over the past several decades. In addition, we explore the recent molecular characterization of these tumors which may point towards new potential avenues of personalized treatment.

  5. Mixed odontogenic tumor: Ameloblastoma and calcifying epithelial odontogenic tumor

    Directory of Open Access Journals (Sweden)

    Etit Demet

    2010-01-01

    Full Text Available Odontogenic tumors constitute a group of heterogeneous disease derived from epithelial, mesenchymal and/or ectomesenchymal elements. Ameloblastoma is the best known and the most frequent form of odontogenic tumors. Calcifying epithelial odontogenic tumor (CEOT, known as Pindborg tumor, is locally invasive lesion which has a characteristic amiloid deposition. Here a case of a peripheral ameloblastoma associated with CEOT is presented with clinical and morphological features.

  6. Expression of RECK and matrix metalloproteinase-2 in ameloblastoma

    Directory of Open Access Journals (Sweden)

    Xie Hong-Liang

    2009-12-01

    Full Text Available Abstract Background Ameloblastoma is a frequent odontogenic benign tumor characterized by local invasiveness, high risk of recurrence and occasional metastasis and malignant transformation. Matrix metalloproteinase-2 (MMP-2 promotes tumor invasion and progression by destroying the extracellular matrix (ECM and basement membrane. For this proteolytic activity, the endogenous inhibitor is reversion-inducing cysteine rich protein with Kazal motifs (RECK. The aim of this study was to characterize the relationship between RECK and MMP-2 expression and the clinical manifestation of ameloblastoma. Methods Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR were employed to detect the protein and mRNA expression of RECK and MMP-2 in keratocystic odontogenic tumor (KCOT, ameloblastoma and ameloblastic carcinoma. Results RECK protein expression was significantly reduced in KCOT (87.5%, ameloblastoma (56.5% and ameloblastic carcinoma (0% (P Conclusion Low or no RECK expression and increased MMP-2 expression may be associated with negative clinical findings in ameloblastoma. RECK may participate in the invasion, recurrence and malignant transformation of ameloblastoma by regulating MMP-2 at the post-transcriptional level.

  7. Maxillary ameloblastoma: Factors associated with risk of recurrence.

    Science.gov (United States)

    Yang, Rong; Liu, Zheqi; Peng, Canbang; Cao, Wei; Ji, Tong

    2017-05-01

    Maxillary ameloblastomas are rare and associated with an aggressive course because of the anatomic composition of the maxilla and adjacent structures. The risk factors associated with recurrence were analyzed in this retrospective study. Cases of maxillary ameloblastoma reported in a tertiary hospital from 2005 to 2015 were analyzed to identify clinicopathological and radiological factors associated with recurrence using univariate and multivariate Cox regression analyses. A total of 890 patients with ameloblastomas were treated in this study, of whom only 51 (5.7%) had maxillary ameloblastomas. The median follow-up period was 56 ± 28.65 months. Of the maxillary ameloblastomas, 29 (56.8%) were primary and 22 (43.1%) were recurrent. Soft tissue or maxillary sinus invasion and primary or recurrent tumor status were risk factors significantly associated with recurrence in the univariate analyses (p = .006 vs p = .025, respectively), whereas the association between recurrence and surgical methods was borderline significant (p = .08). The multivariate Cox regression analysis showed that soft tissue or maxillary sinus involvement was significantly associated with recurrence (p = .023). Recurrent tumors and tumors with soft tissue or maxillary sinus involvement were risk factors for recurrence among patients with maxillary ameloblastoma. © 2017 Wiley Periodicals, Inc. Head Neck 39: 996-1000, 2017. © 2017 Wiley Periodicals, Inc.

  8. Differential diagnosis between ameloblastoma and odontogenic keratocyst using computed tomography

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    Kawazu, Toshiyuki; Yoshiura, Kazunori; Yuasa, Kenji; Kanda, Shigenobu; Ohzeki, Satoru; Shinohara, Masanori [Kyushu Univ., Fukuoka (Japan). Faculty of Dentistry; Araki, Kazuyuki; Higuchi, Yoshinori

    1997-09-01

    Radiographic features of ameloblastoma and odontogenic keratocyst are similar although their biological behaviors are different. Therefore, it is very important to differentiate these lesions before treatment. The aim of this study was to differentiate between the two lesions based on characteristic CT findings. Fifty-three patients diagnosed histopathologically as having ameloblastoma (29 cases) or odontogenic keratocyst (24 cases) and who underwent CT were evaluated radiologically, and some characteristic radiological features to differentiate both lesions were assessed. Ameloblastomas tended to be more than 40 mm in long diameter and 0.5 short/long diameter ratio, whereas odontogenic keratocysts were less than 40 mm and 0.5, respectively (P<0.05: Chi-square test). Buccal expansion of the cortex was seen more often in ameloblastomas than in odontogenic keratocysts (P<0.05: Chi-square test). Together with these features and internal high density structure, which was only seen in odontogenic keratocysts, differential diagnostic criteria between ameloblastomas and odontogenic keratocysts were proposed. Diagnostic accuracy based on these criteria was 84.9%. These results that our criteria to differentiate ameloblastomas from odontogenic keratocysts using CT are clinically useful prior to treatment. (author)

  9. Management of recurrent ameloblastoma of the jaws; a 40-year single institution experience

    NARCIS (Netherlands)

    Hertog, D.; Schulten, E.A.J.M.; Leemans, C.R.; Winters, H.A.H.; van der Waal, I.

    2011-01-01

    Ameloblastoma is a histologically almost always benign odontogenic tumor with a high rate of recurrence if not removed completely. Therefore, radical surgery is the treatment of choice of a primary ameloblastoma. Of 18 patients with a recurrent ameloblastoma, previously treated by enucleation,

  10. PERIPHERAL AMELOBLASTOMA - A CASE-REPORT AND REVIEW OF THE LITERATURE

    NARCIS (Netherlands)

    NAUTA, JM; PANDERS, AK; SCHOOTS, CJF; VERMEY, A; ROODENBURG, JLN

    Peripheral ameloblastoma is a benign odontogenic tumor with the same histological characteristics as the centrally located ameloblastoma, but appearing in the gingiva and mucosa of the tooth-bearing area of the jaws. A review is presented of 53 cases: 45 being reported as peripheral ameloblastoma

  11. Ameloblastoma: 25 Year Experience at a Single Institution.

    Science.gov (United States)

    Milman, Tatyana; Ying, Gui-Shuang; Pan, Wei; LiVolsi, Virginia

    2016-12-01

    Ameloblastoma is a rare, locally aggressive odontogenic neoplasm, accounting for fewer than 1 % of head and neck tumors. Recent literature suggests that the initial surgical approach and histologic growth patterns are the most important prognostic determinants in ameloblastoma. The aim of this study was to compare the clinical presentation, management, and outcomes of patients with ameloblastoma with data reported in the literature; the study spanned 2 decades at a single institution. The institution's database was searched for all patients with pathologically confirmed ameloblastoma, diagnosed between 1990 and 2015. The data collected included sex, age, clinical and imaging findings, management, histologic pattern, clearance of surgical margins, length of follow-up, time to recurrence, and disease-related mortality. The potential risk factors of recurrence were evaluated using log-rank test, proportional hazard model, and Fisher exact test. Review of the database yielded 54 patients with pathologically confirmed ameloblastoma and follow-up. Recurrence was noted in 13 (24 %) patients. Surgical approach was associated with the risk of recurrence (6.1 % following radical resection vs. 52 % following limited surgery, p = 0.002). There were trends toward higher recurrence rate in the group with pathologically documented positive margins (p = 0.054) and in follicular ameloblastoma (p = 0.35). Transformation into ameloblastic carcinoma was identified in two patients. There was no disease-related mortality. Our study confirms the recent data regarding the importance of radical surgical resection in management of ameloblastoma. Surgical approach appears to be the strongest predictor of tumor clearance.

  12. MicroRNAs expression profile in solid and unicystic ameloblastomas.

    Directory of Open Access Journals (Sweden)

    A Setién-Olarra

    Full Text Available Odontogenic tumors (OT represent a specific pathological category that includes some lesions with unpredictable biological behavior. Although most of these lesions are benign, some, such as the ameloblastoma, exhibit local aggressiveness and high recurrence rates. The most common types of ameloblastoma are the solid/multicystic (SA and the unicystic ameloblastoma (UA; the latter considered a much less aggressive entity as compared to the SA. The microRNA system regulates the expression of many human genes while its deregulation has been associated with neoplastic development. The aim of the current study was to determine the expression profiles of microRNAs present in the two most common types of ameloblastomas.MicroRNA expression profiles were assessed using TaqMan® Low Density Arrays (TLDAs in 24 samples (8 SA, 8 UA and 8 control samples. The findings were validated using quantitative RTqPCR in an independent cohort of 19 SA, 8 UA and 19 dentigerous cysts as controls.We identified 40 microRNAs differentially regulated in ameloblastomas, which are related to neoplastic development and differentiation, and with the osteogenic process. Further validation of the top ranked microRNAs revealed significant differences in the expression of 6 of them in relation to UA, 7 in relation to SA and 1 (miR-489 that was related to both types.We identified a new microRNA signature for the ameloblastoma and for its main types, which may be useful to better understand the etiopathogenesis of this neoplasm. In addition, we identified a microRNA (miR-489 that is suggestive of differentiating among solid from unicystic ameloblastoma.

  13. Hybrid Ameloblastoma of the Maxilla: A Puzzling Pathology

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    Chintamaneni Raja Lakshmi

    2016-07-01

    Full Text Available Ameloblastomas are slow growing, locally invasive, benign odontogenic tumors of an epithelial origin, accounting for approximately 1% of all oral tumors. A 40-year-old man presented with a chief complaint of a swelling over the left side of his face of 4 years’ duration. On examination, gross facial asymmetry was detected, and a well-defined swelling was noted intraorally involving the left maxilla medially from the mid palatal raphe and obliterating the buccal vestibule laterally. The swelling was non-tender and exhibited dual consistencies: firm in the palate and cystic in the vestibular region. Computed tomography revealed a multilocular radiolucency, which involved the left maxilla, encroached into the left maxillary sinus and the nasal complex, and caused bony erosion. Early diagnosis and treatment are the key tools in managing ameloblastomas, failure of which may lead to a significant deterioration of the prognosis and an increased recurrence rate. Uncommon variants of ameloblastomas have been gaining interest recently. To date, 25 cases of hybrid ameloblastomas have been documented in the scientific literature. We present an extremely rare hybrid type of the ameloblastoma with combined follicular, cystic, acanthomatous, and desmoplastic variants, which render it the first of its kind to have ever been reported.

  14. Acanthomatous ameloblastoma with atypical foci in five dogs.

    Science.gov (United States)

    Malmberg, Jennifer L; Howerth, Elizabeth W; Powers, Barb E; Schaffer, Paula A

    2017-03-01

    Acanthomatous ameloblastoma is a common, locally invasive, nonmetastasizing tumor of the canine oral cavity. The long-term prognosis for canine acanthomatous ameloblastoma is good if complete excision can be achieved, usually by maxillectomy or mandibulectomy. A variant of acanthomatous ameloblastoma with atypical foci was noted in 5 dogs. There was no age, breed, or sex predisposition. Atypical cells were pleomorphic with a high mitotic rate. They were immunohistochemically negative for cytokeratin, vimentin, melan A, PNL2, CD3, Pax5, CD18, chromogranin A, and synaptophysin. Ultrastructurally, the atypical cells had modest amounts of electron-lucent cytoplasm, abundant rough endoplasmic reticulum, zonula adherens junctions, cleaved or irregular nuclei, and occasional cytoplasmic structures consistent with secretory granules or lysosomes. Complete excision was achieved by maxillectomy or mandibulectomy in 3 dogs; the lesion was incompletely excised in 2 dogs. No ancillary therapy was elected in any patient. No local recurrence or distant metastasis was reported in any case. One patient died of heart failure 20 mo following complete excision; all other patients were alive at last follow-up (average follow-up: 18.8 mo, range: 6-30 mo). The histogenesis of the atypical foci is unclear, but atypical foci within acanthomatous ameloblastoma do not appear to be associated with metastasis or with a poor prognosis relative to acanthomatous ameloblastoma with typical histologic morphology.

  15. Molecular concepts in the pathogenesis of ameloblastoma: implications for therapeutics.

    Science.gov (United States)

    Jhamb, Tania; Kramer, Jill M

    2014-12-01

    Ameloblastoma is a benign odontogenic neoplasm that may exhibit aggressive biological behavior as evidenced by its rapid growth and significance recurrence rates following initial surgical resection. Currently, the only therapy for ameloblastoma is surgical, and adjunctive treatment modalities are needed to mitigate tumor growth and to reduce the need for extensive and disfiguring surgeries. Many studies have identified markers expressed by ameloblastoma and these lend insight to our understanding of tumor progression. This review provides a summary of the specific molecular pathways implicated in tumor pathogenesis, including those involved in bone remodeling, apoptosis, cell signaling, and tumor suppression. Based on these data, we identify several prognostic or therapeutic markers that have been used successfully in the treatment of other neoplastic processes that may also have diagnostic and prognostic utility for ameloblastoma. Thus, it is important to determine which markers hold the greatest promise for clinical management of this benign neoplasm in order to improve treatment options, particularly in patients with aggressive forms of ameloblastoma. Copyright © 2014 Elsevier Inc. All rights reserved.

  16. A case report on desmoplastic ameloblastoma of anterior mandible.

    Science.gov (United States)

    Sharma Lamichhane, Narayan; Liu, Qilin; Sun, Hongchen; Zhang, Wei

    2016-03-16

    Desmoplastic ameloblastoma (DA) is a rare variant that accounts for approximately 4-13% of ameloblastoma, displaying significant differences in anatomical site, imaging, and histologic appearance. It has been included in WHO classification of head and neck tumor (WHO-2005) as a variant of ameloblastoma. The tumor resembles benign fibro-osseous lesion for being frequently occurring in the anterior region of jaws as a mixed radiopaque-radiolucent lesion. We present a case of DA in a 43-year-old female with a painless swelling in the anterior region of mandible. No fluid was evident on fine needle aspiration. A mixed lesion with multilocular appearance was evident on both panoramic radiographs as well as computed tomography scan. An incisional biopsy confirmed it to be a case of desmoplastic ameloblastoma. Segmental mandibulectomy was performed from teeth 35 to 44. The patient is on routine follow-up and is currently free of ailment. The present case deserves emphasis because of its unfamiliar appearance, potentially aggressive nature and deceptive radiologic appearance maximizing the chances of misdiagnosis. So, the clinician should be alert enough to include desmoplastic ameloblastoma in differential diagnosis of any lesion/growth with mixed radiolucent-radiopaque appearance having ill-defined borders and occurring in anterior maxilla or mandible.

  17. Suppression of local invasion of ameloblastoma by inhibition of matrix metalloproteinase-2 in vitro

    Science.gov (United States)

    Wang, Anxun; Zhang, Bin; Huang, Hongzhang; Zhang, Leitao; Zeng, Donglin; Tao, Qian; Wang, Jianguang; Pan, Chaobin

    2008-01-01

    Background Ameloblastomas are odontogenic neoplasms characterized by local invasiveness. This study was conducted to address the role of matrix metalloproteinase-2 (MMP-2) in the invasiveness of ameloblastomas. Methods Plasmids containing either MMP-2 siRNA or tissue inhibitor of metalloproteinase-2 (TIMP-2) cDNA were created and subsequently transfected into primary ameloblastoma cells. Zymography, RT-PCR, and Western blots were used to assess MMP-2 activity and expression of MMP-2 and TIMP-2, as well as protein levels. Results Primary cultures of ameloblastoma cells expressed cytokeratin (CK) 14 and 16, and MMP-2, but only weakly expressed CK18 and vimentin. MMP-2 mRNA and protein levels were significantly inhibited by RNA interference (P ameloblastoma. Conclusion These results indicate that inhibition of MMP-2 activity suppresses the local invasiveness of ameloblastoma cells. This mechanism may serve as a novel therapeutic target in ameloblastomas pursuant to additional research. PMID:18588710

  18. Unicystic plexiform ameloblastoma with mural proliferation: a full-blown lesion.

    Science.gov (United States)

    Anchlia, Sonal; Bahl, Sumit; Vyas, Siddharth; Raju, Godishala Swamy Sugunakar

    2016-04-06

    Ameloblastoma is the most common aggressive benign odontogenic tumour of the jaws and has received considerable attention due to its frequency, clinical subtypes and high tendency to infiltrate and recur. There are various types of this tumour and confusion still exists among clinicians as to its correct classification. Multicystic ameloblastoma is the most frequent subtype while unicystic ameloblastoma can be considered a variant of the solid or multicystic subtype. Unicystic ameloblastoma is considered a less aggressive tumour with a variable recurrence rate. However, its frequency is often underestimated. Ameloblastoma is often asymptomatic, presenting as a slowly enlarging facial swelling or an incidental finding on radiograph. It is seen in all age groups but is most commonly diagnosed in the third and fourth decades. We report a case of unusual unicystic ameloblastoma and present its clinical, radiological and full-blown histological changes and treatment modalities, providing new insights into ameloblastomas. 2016 BMJ Publishing Group Ltd.

  19. Metastasizing Ameloblastoma With Localized Interstitial Spread in the Lung: Report of Two Cases.

    Science.gov (United States)

    Chou, Yueh-Hung; Jhuang, Jie-Yang; Chang, Min-Hsiang; Huang, Wen-Chih; Hsieh, Min-Shu

    2014-06-01

    Ameloblastoma is a locally aggressive, epithelial odontogenic tumor involving mandibles and maxillas. Distant metastasis is a very rare condition and is designated as metastasizing (malignant) ameloblastoma despite its benign histological appearance. Up to now, only 27 well-documented cases of metastasizing ameloblastomas are reported in the literature, and lung is the most commonly involved organ. In previous reports of pulmonary metastasizing ameloblastomas, there was little description of the histopathologic finding. Here, the authors report 2 cases of pulmonary metastasizing ameloblastomas with special emphasis on their interesting, interstitial spread along alveolar septa, resulting in a unique 2-cell pattern under microscopic examination. Pulmonary metastasizing ameloblastoma may pose difficulty in diagnosis if the pathologist is not aware of patient's clinical history of ameloblastoma. © The Author(s) 2013.

  20. Desmoplastic variant of ameloblastoma of the maxilla: A case report

    Energy Technology Data Exchange (ETDEWEB)

    Koh, Kwang Joon; Park, Ha Na; Kim, Kyoung A [Oral and Maxillofacial Radiology, School of Dentistry and Institute of Oral Bioscience, Chonbuk National University, Jeonju (Korea, Republic of)

    2015-12-15

    The desmoplastic variant of ameloblastoma is a rare form of ameloblastoma characterized by unique radiographic and histologic features. A 46-year-old female was referred to our hospital, complaining of swelling in the left upper lip area. Radiographic findings revealed an ill-defined multilocular lesion with a large cystic lesion and thick sclerotic trabeculae on the left anterior maxilla. After the patient underwent partial osteotomy, histologic analysis revealed a desmoplastic ameloblastoma with no evidence of a hybrid lesion or cyst formation. The radiographic findings in the present case were different from those described in previous case reports. These findings are of special importance due to the unfamiliar radiographic and histologic features of this lesion.

  1. Clinicoradiologic Differential Diagnosis of Odontogenic Keratocyst and Ameloblastoma

    International Nuclear Information System (INIS)

    Jeong, Ho Gul; Lee, Jang Yeol; Kim, Kee Deog; Park, Chang Seo

    2000-01-01

    To clarify the clinical and radiologic parameters that can be used to differentiate odontogenic keratocyst and ameloblastoma. The records of 46 patients of ameloblstoma and 48 patients of odontogenic keratocyst at the Yonsei University Dental Hospital during the period of 1979 to 1995 were retrospectively reviewed. As a possible means for differentiating between the odontogenic keratocyst and ameloblastoma, the clinical parameters and the radiologic parameters were evaluated. In the clinical parameters, there was no significant deference in age, sex, and sign and symptoms (p>0.05).In the radiologic parameters, there was significant difference in site, shape of the lesion, and external root resorption of adjacent teeth (p<0.05). The site, shape of the lesion, and external root resorption of adjacent teeth can be the parameters to differentiate odontogenic keratocyst and ameloblastoma, but a definite differentiation of these two lesions needs a more specialized imaging modality.

  2. Desmoplastic variant of ameloblastoma of the maxilla: A case report

    International Nuclear Information System (INIS)

    Koh, Kwang Joon; Park, Ha Na; Kim, Kyoung A

    2015-01-01

    The desmoplastic variant of ameloblastoma is a rare form of ameloblastoma characterized by unique radiographic and histologic features. A 46-year-old female was referred to our hospital, complaining of swelling in the left upper lip area. Radiographic findings revealed an ill-defined multilocular lesion with a large cystic lesion and thick sclerotic trabeculae on the left anterior maxilla. After the patient underwent partial osteotomy, histologic analysis revealed a desmoplastic ameloblastoma with no evidence of a hybrid lesion or cyst formation. The radiographic findings in the present case were different from those described in previous case reports. These findings are of special importance due to the unfamiliar radiographic and histologic features of this lesion

  3. Unicystic ameloblastoma with diverse mural proliferation - a hybrid lesion

    International Nuclear Information System (INIS)

    Mahadesh, Jyothi; Rayapati, Dilip Kumar; Maligi, Prathima M; Ramachandra, Prashanth

    2011-01-01

    A 46-year-old man was referred to our hospital for treatment, complaining of swelling on the right mandibular molar region. Radiographic examination revealed a well defined multilocular radiolucent lesion with root resorption of right lower anteriors and molars. Following biopsy, a diagnosis of unicystic ameloblastoma of mural type was made and hemimandibulectomy was performed under general anesthesia. Histopathological examination of the surgical specimen exhibited a unicystic ameloblastoma of luminal, intraluminal, and mural type. Intraluminal proliferation was of plexiform pattern and mural proliferation showed unusual histopathological findings, which revealed follicular, acanthomatous areas coexisted with desmoplastic areas. This mural picture was similar to the so-called 'hybrid lesion of ameloblastoma', whose biological profile is not elicited due to the lack of adequate published reports. Two years follow up till date has not revealed any signs of recurrence.

  4. Giant ameloblastoma mortality; a consequence of ignorance, poverty and fear.

    Science.gov (United States)

    Etetafia, Mabel Okiemute; Arisi, Andrew Arotuah; Omoregie, Osawe F

    2014-05-22

    Ameloblastoma is a benign tumour of odontogenic origin. Although locally invasive, delay in treatment can lead to severe disfiguring of the facial region. In this report, we present two cases, a man and a woman, of a large ameloblastoma that presented with the typical radiographic features of variably sized radiolucent loculations. Patients presented initially with relatively small tumours but due to fear of surgery, ignorance and possibly poverty, tumour growth became enormous. The female patient died while awaiting consent for surgery and the male patient died at home due to complications closely associated with fear and growth of tumour. Literatures on mortality of ameloblastoma are reviewed. The need for more public enlightenment on the nature and treatment of facial tumours is highlighted. 2014 BMJ Publishing Group Ltd.

  5. Unicystic ameloblastoma with diverse mural proliferation - a hybrid lesion

    Energy Technology Data Exchange (ETDEWEB)

    Mahadesh, Jyothi [Sri Siddhartha Dental College and Hospital, (India); Rayapati, Dilip Kumar; Maligi, Prathima M; Ramachandra, Prashanth [Dayananda Sagar College of Dental Sciences, Bengaluru (India)

    2011-03-15

    A 46-year-old man was referred to our hospital for treatment, complaining of swelling on the right mandibular molar region. Radiographic examination revealed a well defined multilocular radiolucent lesion with root resorption of right lower anteriors and molars. Following biopsy, a diagnosis of unicystic ameloblastoma of mural type was made and hemimandibulectomy was performed under general anesthesia. Histopathological examination of the surgical specimen exhibited a unicystic ameloblastoma of luminal, intraluminal, and mural type. Intraluminal proliferation was of plexiform pattern and mural proliferation showed unusual histopathological findings, which revealed follicular, acanthomatous areas coexisted with desmoplastic areas. This mural picture was similar to the so-called 'hybrid lesion of ameloblastoma', whose biological profile is not elicited due to the lack of adequate published reports. Two years follow up till date has not revealed any signs of recurrence.

  6. Clinicoradiologic Differential Diagnosis of Odontogenic Keratocyst and Ameloblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Ho Gul; Lee, Jang Yeol; Kim, Kee Deog; Park, Chang Seo [Dept. of Oral and Maxillofacial Radiology, College of Dentistry, Yensei University, Seoul (Korea, Republic of)

    2000-12-15

    To clarify the clinical and radiologic parameters that can be used to differentiate odontogenic keratocyst and ameloblastoma. The records of 46 patients of ameloblstoma and 48 patients of odontogenic keratocyst at the Yonsei University Dental Hospital during the period of 1979 to 1995 were retrospectively reviewed. As a possible means for differentiating between the odontogenic keratocyst and ameloblastoma, the clinical parameters and the radiologic parameters were evaluated. In the clinical parameters, there was no significant deference in age, sex, and sign and symptoms (p>0.05).In the radiologic parameters, there was significant difference in site, shape of the lesion, and external root resorption of adjacent teeth (p<0.05). The site, shape of the lesion, and external root resorption of adjacent teeth can be the parameters to differentiate odontogenic keratocyst and ameloblastoma, but a definite differentiation of these two lesions needs a more specialized imaging modality.

  7. Tissue microarray use for immunohistochemical study of ameloblastoma.

    Science.gov (United States)

    Neves-Silva, Rodrigo; Fonseca, Felipe Paiva; de Jesus, Adriana Souza; Pontes, Hélder Antônio Rebelo; Rocha, André Caroli; Brandão, Thais Bianca; Vargas, Pablo Agustin; Lopes, Márcio Ajudarte; de Almeida, Oslei Paes; Santos-Silva, Alan Roger

    2016-10-01

    Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. Forty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). Kappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). Using a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  8. Cystic ameloblastoma--behavior and treatment of 21 cases.

    Science.gov (United States)

    Rosenstein, T; Pogrel, M A; Smith, R A; Regezi, J A

    2001-11-01

    This article presents a series of cystic ameloblastomas in which an unexpected capacity for bony destruction and recurrence was shown. Proliferation rates were evaluated to see if there is a correlation to the biologic behavior of these lesions. Clinical and histologic material on 21 consecutive cystic ameloblastomas was retrieved and reviewed. Immunohistochemical analysis of proliferation-associated Ki-67 protein was carried out to determine mitotic indices for 10 cystic ameloblastomas, and these were compared to 10 solid ameloblastomas and 10. dentigerous cysts. Lesions from 10 males and 11 females (age range, 12 to 72 years; mean age, 35 years) were included. All lesions were in the mandible; 18 in posterior sites. Lesion size ranged from 2 to 8 cm in greatest dimension. Cortical perforation was evident in 7 lesions, and multilocularity (more often in older patients) was evident in 6 lesions. Recurrences were seen in 9 cases (43%), and the time between initial treatment and recurrence was as long as 10 years. The characteristic histopathologic feature was a thin, stratified squamous cystic lining with spongiosis and basal palisades. Ten cases also showed mural invasion, and 4 had plexiform luminal proliferation. The proliferation rate of the cystic ameloblastomas (represented as a percentage of cells in cell cycle) was 4.3%, compared with solid tumors at 2.8% and dentigerous cysts at 6.6%. Cystic ameloblastomas occur within a wide age range, but at slightly lower mean age than solid lesions. There is a very strong predilection for the mandible, and there appears to be no gender difference. Lesions frequently become large, destructive, and/or multilocular. There is a significant recurrence potential, and extended follow-up is advisable. The deceptively innocent histology of cystic ameloblastomas belies the biologic potential of these lesions. The mechanism(s) by which cystic ameloblastomas gain their destructive behavior seems less likely associated with

  9. Nuclear morphometry in canine acanthomatous ameloblastomas and squamous cell carcinomas

    Directory of Open Access Journals (Sweden)

    M Martano

    2009-06-01

    Full Text Available The aim of this study was to evaluate whether morphometrical analysis can be of diagnostic value for canine acanthomatous ameloblastoma. We calculated, by means of an automated image analyser, some morphometric nuclear parameters, in particular: mean nuclear area (MNA, mean nuclear perimeter (MNP, maximum and minimum diameters (MDx and MDm coefficient of variation of the nuclear area (NACV, largest to smallest dimension ratio (LS ratio, and form factor (FF, in 8 canine acanthomatous ameloblastomas, and we compared these morphometric data to those of 13 squamous cell carcinomas of canine gingiva. The results indicated a progressive increase of the MNA, NACV, MNP and MDm proceeding from acanthomatous ameloblastomas (MNA: 42.11±8.74; NACV: 28,36±7,23; MNP: 24.18± 2.68; MDm: 5.69±0.49 to squamous cell carcinomas (MNA:49,69±9,10; NACV: 30,89±7,75; MNP: 25.63±2.54; MDm: 6.64±0.73. On the contrary, the LS ratio and the FF resulted greater in acanthomatous ameloblastomas (LS ratio: 1,63±0,12; FF: 1,13±0,002 than in SCCs (LS ratio: 1,40±0,12; FF:0.91±0.38. Moreover, the MNA, MNP,MDx and MDm resulted similar (MNA: p=0.89; MNP: p=0,65; MDm: p=0,16; MDx: p=0,13 in a subset of four acanthomatous ameloblastomas with cellular atypia (MNA:49,01±6,88; MNP: 26,28±1,99; MDm: 6.08±0.41; MDx: 10.18±0.88 and in squamous cell carcinomas (MNA:49.69±9,10; MNP: 25.63±2.54; MDm: 6.64±0.73; MDx: 9.26±1.05. While the NACV values resulted higher in typical acanthomatous ameloblastoma (29,99±6,06 than in atypical acanthomatous ameloblastoma (26,74±8,84 and similar to those of the SCCs (30,89±7,75. These results seem to confirm that acanthomatous ameloblastoma is a malignant or potentially malignant lesion and emphasizes that nuclear morphometry analysis can be an useful diagnostic and prognostic method in canine oral pathology.

  10. [Expression of HOXC13 in ameloblastoma].

    Science.gov (United States)

    Zhong, Ming; Wang, Jie; Gong, Yan-bing; Li, Jin-cheng; Zhang, Bo; Hou, Lin

    2007-01-01

    To study the expression of HOXC13 mRNA in ameloblastoma (AB), and to investigate its biological significance. HOXC13 mRNA was examined in 47 cases of AB (primary AB 29 cases, recurrent AB 14 cases, malignant AB 4 cases). 2 cases of fibrous dysplasia of bone, 10 cases of keratocystic odontogenic tumor (KCOT) and 7 cases of normal oral mucosa were selected as control. The positive rates of HOXC13 mRNA in AB, KCOT, and normal oral mucosa were 97.9% (46/47), 7/10 and 3/7, respectively. There was a significant difference among AB, OKC and normal mucosa (chi(2) = 21.665, P = 0.001). For HOXC13, the keratinizing cells and granulizing cells in AB were negative, some fibroblasts were positive, 2 cases of fibrous dysplasia of bone were positive. HOXC13 was highly expressed in AB. The expression of HOXC13 mRNA in AB had heterogeneity, which could improve the epithelial proliferation, and its loss may lead to the cornification and degeneration of epithelial cells.

  11. Recurrent giant mandibular ameloblastoma in young adults.

    Science.gov (United States)

    Gravvanis, Andreas; Koumoullis, Harry D; Anterriotis, Dimitrios; Tsoutsos, Dimosthenis; Katsikeris, Nick

    2016-04-01

    The purpose of the study was to define the most appropriate management of the giant mandibular ameloblastoma (GMA) in young adults. A retrospective study was performed on patients with GMA <30 years old. The data collected included initial treatment, tumor margins, reconstruction, and follow-up. Patients evaluated speech, chewing, swallowing, and facial appearance after definitive treatment. Thirteen patients were identified with recurrent solid/multicystic disease requiring further treatment. Definitive treatment involved segmental mandibulectomy and reconstruction with free fibular flap in all patients. Seven patients had immediate reconstruction (group A) and 6 had secondary (group B). Mandibular resection was planned at least 2 cm beyond the radiological limit, free margins were achieved in all patients, and all flaps were transplanted successfully. In group A, functional score was 13.7 ± 0.45 and facial appearance score was 4.5 ± 0.49, whereas in group B were 11.16 ± 0.37 and 3.3 ± 0.5, respectively (both p < .05). Aggressive resection of the GMA and immediate reconstruction is strongly advised. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1947-E1954, 2016. © 2015 Wiley Periodicals, Inc.

  12. Overexpression of MDM2 protein in ameloblastomas as compared to adenomatoid odontogenic tumor

    Directory of Open Access Journals (Sweden)

    A Krishna

    2012-01-01

    Full Text Available Background: Recent studies on odontogenic tumors have identified various molecular alterations responsible for their development, and determination of epithelial proliferation is a useful means of investigating the differences in biologic behavior of these tumors. One such specific marker to identify proliferative activity and tumor aggressiveness by immunohistochemistry (IHC is MDM2, 90-95kDa protein. Objective: This immunohistochemical study using MDM2 expression was undertaken to understand better the diverse biological activity of two groups of odontogenic tumors namely ameloblastoma and adenomatoid odontogenic tumor (AOT based on their cell proliferation activity. Materials and Methods: A total of 50 cases, comprising of 36 ameloblastoma samples and 14 AOT samples, were subjected to heat-induced antigen retrieval method using citrate buffer in a pressure cooker. Consequently, the sections were stained with MDM2 monoclonal antibody and visualized using an LSAB+ kit. Results: In ameloblastomas, statistically significant association was seen between plexiform ameloblastomas, follicular ameloblastomas with granular cell changes, desmoplastic and unicystic variants. The predominant nuclear staining by MDM2 revealed overexpression in ameloblastomas as compared to AOT. Conclusion: The MDM2 overexpression noticed in plexiform ameloblastoma, follicular ameloblastoma with granular cell changes and acanthomatous ameloblastoma when compared to simple unicystic and desmoplastic ameloblastoma suggest a relatively enhanced proliferative phenotype of these solid multicystic variants of ameloblastomas. On overall comparison, higher expression was noted in ameloblastomas when compared to AOT. This indicates differences in the aggressive nature between these two groups of odontogenic tumors favoring the perception of a greater aggressive nature of ameloblastomas.

  13. A Classification System for Recurrent Ameloblastoma of the Jaws ...

    African Journals Online (AJOL)

    A Classification System for Recurrent Ameloblastoma of the Jaws- Review of 30 Cases in Nigerians. GT Arotiba, AO Effiom, ASO Ayodele, MO Ogundana, MO Gbotolorun, HO Olasoji, O James, AL Ladeinde, VI Ugboko, KC Ndukwe, IC Ikem, RO Braimah ...

  14. A 40-year Retrospective Clinicopathological Study of Ameloblastoma in Iran.

    Science.gov (United States)

    Saghravanian, Nasrollah; Salehinejad, Jahanshah; Ghazi, Narges; Shirdel, Mohammad; Razi, Mahsa

    2016-01-01

    Ameloblastoma is the most common epithelial odontogenic tumor. It may show locally invasive behavior resulting in recurrence and malignancy. Therefore, appropriate diagnosis of this tumor is necessary. The aim of this study was to evaluate clinicopathological characteristics of ameloblastomas in an Iranian population. We present a 40-year retrospective study of patients diagnosed from 1971 to 2010 in the Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mashhad, Iran. Information gathered from patient records included age, gender, tumor location and histologic type. The frequency of odontogenic tumors among all lesions was 2.08% and ameloblastoma with 88 samples demonstrated the greatest prevalence (41.5%). Regarding gender, 60% of samples occurred in males. The mean age of studied patients was 33.02± 15.74 years with a peak of occurrence in the third decade of life. The most frequent location of tumor was the mandibles (93.2%). Eighty five (96.6%) tumors were recorded as benign and 3 (3.4%) as malignant. Of benign tumors, 62 (72.9%), 20 (23.5%) and 3 (3.6%) cases were of conventional, unicyctic and peripheral types, respectively. In contrast to most previous studies, the most common histologic subtype in the present study was plexiform. Knowledge of the incidence of ameloblastoma and its clinicopathologic features including most common location, gender and age distribution in different ethnogeographic backgrounds is necessary for accurate diagnosis and proper treatment.

  15. A single cervical lymph node metastasis of malignant ameloblastoma.

    Science.gov (United States)

    Kim, Yoori; Choi, Sung-Weon; Lee, Jong-Ho; Ahn, Kang-Min

    2014-12-01

    Cervical node metastasis of malignant ameloblastoma is extremely rare. Because of its rarity, there is no standard treatment modality in a single lymph node metastasis in malignant ameloblastoma. Eleven patients of malignant ameloblastoma involving a single cervical lymph node metastasis and one new case were reviewed. Neck treatment was classified into neck dissection and simple excision. Local nodal recurrence, distant metastasis and follow-up periods were investigated. Eight patients were treated with neck dissection (group A) and four patients underwent a simple node excision (group B). Two patients in group A experienced multiple organ metastases such as liver and lung seven months and 13 years after neck dissection respectively. The other patients showed no recurrence and metastasis. In group B, there was no report of a regional neck recurrence and distant metastasis during follow-up of 1-7 years. Multiple nodes metastasis requires a radical neck dissection; however, simple excision with close follow-up may be used in a single node metastasis in malignant ameloblastoma. Copyright © 2014 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

  16. Intra-epithelially entrapped blood vessels in ameloblastoma.

    Science.gov (United States)

    Siar, Chong Huat; Ishak, Ismadi; Ng, Kok Han

    2015-05-01

    The ameloblastoma is a benign but locally aggressive odontogenic neoplasm with a high recurrence rate. While significant progress has been made in our understanding regarding the role of tumoral vasculature relative to the diverse behavioral characteristics of this tumor, no attention has been paid to a distinct subset of blood vessels entrapped within its epithelial compartment. As vascular niches are known to influence tumoral growth, clarification of these vessels is important. The objectives of this study were to investigate the morphologic characteristics of intra-epithelially entrapped blood vessels (IEBVs) in ameloblastoma and to speculate on their relevance. Here, we evaluated the frequency, microvessel density (MVD), morphology, and distribution pattern of IEBVs in 77 ameloblastoma of different subtypes based on their immunoreactivity for endothelial markers (CD34, CD31, CD105), vascular tight junction protein (claudin-5), pericyte [α-smooth muscle actin (α-sma)], and vascular basement membrane (collagen IV). IEBVs were heterogeneously detected in ameloblastoma. Their mean MVD (CD34 = 15.46 ± 7.25; CD31 = 15.8 ± 5.04; CD105 = 0.82 ± 0.51) showed no significant correlation with different subtypes, and between primary and recurrent tumors (P > 0.05). These microvessels may occur as single/clusters of capillary sprouts, or formed compressed branching/non-branching slits entrapped within the epithelial compartment, and in direct apposition with polyhedral/granular neoplastic epithelial cells. They expressed proteins for endothelial tight junctions (claudin-5-positive) and pericytes (α-sma-positive) but had deficient basement membrane (collagen IV weak to absent). Aberrant expression for CD34, CD31, and CD105 in tumor epithelium was variably observed. Although rare in occurrence, identification of IEBVs in ameloblastoma could potentially represent a new paradigm for vascular assessment of this neoplasm. © 2014 John Wiley & Sons A/S. Published by John

  17. BRAFV600E mutation in the diagnosis of unicystic ameloblastoma.

    Science.gov (United States)

    Pereira, Núbia Braga; Pereira, Karuza Maria Alves; Coura, Bruna Pizziolo; Diniz, Marina Gonçalves; de Castro, Wagner Henriques; Gomes, Carolina Cavalieri; Gomez, Ricardo Santiago

    2016-11-01

    Unicystic ameloblastoma, an odontogenic neoplasm, presents clinical and radiographic similarities with dentigerous and radicular cysts, non-neoplastic lesions. It is not always possible to reach a final diagnosis with the incisional biopsy, leading to inappropriate treatment. The BRAFV600E activating mutation has been reported in a high proportion of ameloblastomas. The purpose of the study was to assess the utility of the detection of the BRAFV600E mutation in the differential diagnosis of unicystic ameloblastoma with dentigerous and radicular cysts. Twenty-six archival samples were included, comprising eight unicystic ameloblastomas (UAs), nine dentigerous and nine radicular cysts. The mutation was assessed in all samples by anti-BRAFV600E (clone VE1) immunohistochemistry (IHC) and by TaqMan mutation detection qPCR assay. Sanger sequencing was further carried out when samples showed conflicting results in the IHC and qPCR. Although all UAs (8/8) showed positive uniform BRAFV600E staining along the epithelial lining length, the mutation was not confirmed by qPCR and Sanger sequencing in three samples. Positive staining for the BRAFV600E protein was observed in one dentigerous cyst, but it was not confirmed by the molecular methods. Furthermore, 2/9 dentigerous cysts and 2/9 radicular cysts showed non-specific immunostaining of the epithelium or plasma cells. None of the dentigerous or radicular cysts cases presented the BRAFV600E mutation in the qPCR assay. The BRAFV600E antibody (clone VE1) IHC may show non-specific staining, but molecular assays may be useful for the diagnosis of unicystic ameloblastoma, in conjunction with clinical, radiological and histopathological features. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  18. Clinicopathologic features of ameloblastoma in Kenya: a 10-year audit.

    Science.gov (United States)

    Adeline, Vilembwa L; Dimba, Elizabeth A O; Wakoli, Kevin A; Njiru, Anthony K; Awange, David O; Onyango, John F; Chindia, Mark L

    2008-11-01

    This study describes the clinical and pathologic features of ameloblastomas seen in the 2 main craniofacial treatment centers in Kenya in the 10-year period between January 1995 and December 2005. A total of 184 patient records were analyzed for this study. Eighty-two (44.6%) of the patients were male, and 102 (55.4%) were female with an overall age range of 10 to 80 years (mean, 30.2 years; SD, 14.1 years). There was no significant difference in gender presentation of ameloblastomas, although females presented at a slightly older age. The mean age for males was 29.9 years, and for females, it was 30.5 years. Patients generally tended to seek medical advice late, with the mean duration at first presentation of 46.3 months for males and 44.4 months for females. Most of the ameloblastomas (n = 172; 93.5%) were located in the mandible, 11 (6.0%) were in the maxilla, and 1 (0.5%) was in the soft tissues. Presenting symptoms included swelling (n = 182; 98.9%), pain (n = 64; 36.0%), mobile teeth/history of extraction (n = 104; 57.5%), purulent discharge (n = 39; 21.7%) and paresthesia (n = 10; 5.6%). The posterior mandible was the most commonly affected site, whereas maxillary ameloblastomas tended to occur in anterior sites. One hundred fifty-three ameloblastomas (83.2%) were of the solid/multicystic subtype; 8 (5.3%) were unicystic; 1 (0.5%) was of extraosseous origin; 1 (0.5%) was desmoplastic; 9 (6.0%) were malignant, and 12 of the records had no histopathologic pattern specified.

  19. Acanthomatous ameloblastoma of mandible crossing the midline: a rare case report.

    Science.gov (United States)

    Bansal, Neha; Sheikh, Soheyl; Bansal, Richa; Sabharwal, Robin; Gupta, Aanchal; Goyal, Ankit; Kainth, Nitika

    2015-01-01

    Ameloblastoma is the most common aggressive benign odontogenic tumor of the jaws. Ameloblastoma is a benign epithelial odontogenic tumor that typically arises in the mandible or maxilla or, rarely, in the immediate adjacent soft tissues. A clinical, radiographic and histopathological report is presented of a case of acanthomatous ameloblastoma in relation to molar in the left mandible of a 30-year-old healthy male. The histopathological examination of the removed specimen revealed the histopathological pattern of an acanthomatous ameloblastoma. The radiographic appearance of the lesion showed the presence of multilocular radiolucencies, which were crossing the midline, which is rarely found in ameloblastoma. Due to its rarity and lack of data, we take this opportunity to present a world first case of acanthomatous ameloblastoma which was crossing the midline.

  20. Hybrid Desmoplastic/Follicular Ameloblastoma of the Mandible: A Case Report and Review of the Literature

    Directory of Open Access Journals (Sweden)

    Masayasu Iwase

    2017-01-01

    Full Text Available Desmoplastic ameloblastoma (DA is one of the 6 histopathological subtypes of ameloblastoma. Hybrid lesions in which histopathologically conventional ameloblastoma coexists with areas of DA are rare. A 40-year-old male was referred to our hospital complaining of a swelling in the right premolar region of the mandible. A panoramic radiograph showed an area of radiolucency with a well-defined corticated border, whereas computed tomography revealed a unilocular radiolucent lesion and buccal expansion together with cortical perforation. The lesion was treated via enucleation and curettage of the marginal bone and fenestration. A histopathological examination showed a hybrid ameloblastoma with a pronounced desmoplastic pattern and follicular changes. The patient’s postoperative course has been favorable up to now, and no marked changes have been observed. We presented a case of hybrid ameloblastoma and reviewed the 36 reported cases of hybrid ameloblastoma that have been reported in the English literature.

  1. Hybrid Desmoplastic/Follicular Ameloblastoma of the Mandible: A Case Report and Review of the Literature

    Science.gov (United States)

    Fukuoka, Airi; Tanaka, Yoko; Saida, Naoyuki; Onaka, Eriko; Bando, Sanae; Kondo, Gen

    2017-01-01

    Desmoplastic ameloblastoma (DA) is one of the 6 histopathological subtypes of ameloblastoma. Hybrid lesions in which histopathologically conventional ameloblastoma coexists with areas of DA are rare. A 40-year-old male was referred to our hospital complaining of a swelling in the right premolar region of the mandible. A panoramic radiograph showed an area of radiolucency with a well-defined corticated border, whereas computed tomography revealed a unilocular radiolucent lesion and buccal expansion together with cortical perforation. The lesion was treated via enucleation and curettage of the marginal bone and fenestration. A histopathological examination showed a hybrid ameloblastoma with a pronounced desmoplastic pattern and follicular changes. The patient's postoperative course has been favorable up to now, and no marked changes have been observed. We presented a case of hybrid ameloblastoma and reviewed the 36 reported cases of hybrid ameloblastoma that have been reported in the English literature. PMID:28584671

  2. Investigation of basement membrane proteins in a case of granular cell ameloblastoma

    Science.gov (United States)

    Lapthanasupkul, Puangwan; Poomsawat, Sopee; Chindasombatjaroen, Jira

    2012-01-01

    Granular cell ameloblastoma is a rare, benign neoplasm of the odontogenic epithelium. A case of massive granular cell ameloblastoma in a 44-year-old Thai female is reported. Histopathological features displayed a follicular type of ameloblastoma with an accumulation of granular cells residing within the tumor follicles. After treatment by partial mandibulectomy, the patient showed a good prognosis without recurrence in a 2-year follow-up. To characterize the granular cells in ameloblastoma, we examined the expression of basement membrane (BM) proteins, including collagen type IV, laminins 1 and 5 and fibronectin using immunohistochemistry. Except for the granular cells, the tumor cells demonstrated a similar expression of BM proteins compared to follicular and plexiform ameloblastomas in our previous study, whereas the granular cells showed strong positivity to laminins 1 and 5 and fibronectin. The increased fibronectin expression in granular cells suggests a possibility of age-related transformation of granular cells in ameloblastoma. PMID:22361945

  3. Malignant ameloblastoma (metastatic ameloblastoma) in the lung: 3 cases of misdiagnosis as primary lung tumor with a unique growth pattern.

    Science.gov (United States)

    Bi, Rui; Shen, Lei; Zhu, Xiongzeng; Xu, Xiaoli

    2015-07-25

    Malignant ameloblastoma (metastatic ameloblastoma, MA) is currently defined as a distinct pathologic entity, MA, despite its histologically benign appearance. According to the new criteria, the histological and clinical features of MA are more homogenous. Here, we report three cases of histologically confirmed pulmonary MA. Two of the three patients complained of chest pain as the primary symptom, and the other case was detected upon the evaluation of pulmonary nodules found during a health examination after a local recurrence of mandible ameloblastoma. All three patients were female with an average age of 48 years. The intervals between the primary ameloblastoma and metastasis to the lung were 14 years, 19 years and 10 years, averaging 14.3 years. Prior to metastasis to the lung, only one patient experienced local recurrences, at 5 and 19 years after the primary tumor resection, while the other two patients both remained disease-free. Computed tomography (CT) or X-ray evaluation demonstrated multiple bilateral lung nodules ranging in size from several millimeters up to 2 cm. Histologically, the pulmonary metastatic tumors showed a unique growth pattern: the tumor cells grew among the interstitial alveoli but did not appear to destructively infiltrate the surrounding tissue. Immunohistochemically, the MA cells expressed squamous differentiation markers, such as CK10/13 and p63, while the alveolar epithelial cells stained for TTF1 and PE10. In this paper, we discuss the clinical behavior, differential diagnosis and unique growth pattern of pulmonary MA.

  4. Interplay Between MMP-9 and TIMP-2 Regulates Ameloblastoma Behavior and Tooth Morphogenesis.

    Science.gov (United States)

    Nunia, Kalpana; Urs, Aadithya B; Kumar, Priya

    2016-01-01

    Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) have been implicated in the local invasiveness of ameloblastoma. This study aims to assess the role of MMP-9 and TIMP-2 in regulating tumor progression in ameloblastomas, taking tooth germs as control. Formalin-fixed, paraffin-embedded tissue sections of 4 tooth germs and 32 ameloblastomas were immunohistochemically examined using antibodies against MMP-9 and TIMP-2. Strong MMP-9 positivity was seen in the epithelial component in both controls and solid multicystic ameloblastoma. Statistically significant difference was observed in the mean stromal MMP-9 immunoscores between follicular, acanthomatous, and granular ameloblastoma when compared with the tooth germ (P=0.004). TIMP-2 expression in the epithelial and mesenchymal components of solid multicystic ameloblastoma and tooth germ was weak as compared with MMP-9 expression. Highest mean epithelial TIMP-2 immunoscore was observed in follicular ameloblastoma and the difference was statistically significant between follicular and granular ameloblastoma (P=0.05). The comparison of mean stromal TIMP-2 immunoscores showed statistically significant difference between follicular subtype and tooth germ (P=0.048), with tooth germ showing least expression among the groups studied. Strong stromal expression of MMP-9 in ameloblastoma compared with tooth germ mesenchyme indicated the possibility of tumor induction with release of growth factors and cytokines, resulting in invasiveness of ameloblastoma. Epithelial TIMP-2 expression was associated with the least and most aggressive behavior of follicular and granular cell ameloblastoma, respectively. Stromal TIMP-2 expression reflected its role in regulating tumor progression in ameloblastoma and in regulating developmental processes in tooth germs by their inhibitory effect on MMP-9.

  5. Metastasizing Ameloblastoma - a perennial pathological enigma? Report of a case and review of literature.

    Science.gov (United States)

    Jayaraj, Gifrina; Sherlin, Herald Justin; Ramani, Pratibha; Premkumar, Priya; Natesan, Anuja; Ramasubramanian, Abilasha; Jagannathan, Nithya

    2014-09-01

    The Ameloblastoma is a slow growing locally invasive odontogenic epithelial neoplasm with a high recurrence rate and a low tendency to metastasize. Metastasis in Ameloblastoma was first described by Simmons and Emura in the 1920s. Slootweg and Muller proposed the term Malignant Ameloblastoma to describe a well-differentiated ameloblastoma that metastasizes but maintains the characteristic cytologic features of the original tumour and the term Ameloblastic Carcinoma to an ameloblastoma with malignant cytological features. About 2% of ameloblastomas undergo metastasis. So far there have only been two cases of Metastasizing Ameloblastoma reported from the Indian Subcontinent. We present the case of a 22-year-old male Indian patient, who presented with a diffuse swelling in the left posterior mandible. Radiographs revealed a multilocular radiolucency in the left mandible. On histopathological examination, the lesion was diagnosed as follicular ameloblastoma. Four years later the patient presented with a swelling in the left submandibular region. Histological examination revealed metastatic ameloblastoma within the cervical lymph node. Copyright © 2013 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

  6. Acanthomatous ameloblastoma in anterior mandibular region of a young patient: A rare case report.

    Science.gov (United States)

    Ugrappa, Sridevi; Jain, Ajay; Fuloria, Neeraj Kumar; Fuloria, Shivkanya

    2017-01-01

    Ameloblastoma is the most known of the epithelial odontogenic benign tumor. It is slow growing and locally aggressive in nature and most commonly seen in the posterior mandible. Various histopathological variants exist, among which acanthomatous type of ameloblastoma is one of the rarest types. Acanthomatous ameloblastoma is usually seen in older aged human population and most commonly reported in canine region of dogs in literature. Here, we report a rare case of acanthomatous ameloblastoma in a young male patient involving mandibular anterior region crossing the midline with recurrence over a period of 2 years of follow-up after surgical resection.

  7. Molecular and genetic aspects in the etiopathogenesis of ameloblastoma: An update

    Science.gov (United States)

    Nagi, Ravleen; Sahu, Shashikant; Rakesh, N

    2016-01-01

    Ameloblastoma is the second most common benign epithelial odontogenic tumor and though it is of a benign nature, it is locally invasive, has a high recurrence rate and could potentially become malignant. Many theories have been proposed to explain the pathogenesis of ameloblastoma. Proper understanding of the pathogenic mechanism involved in ameloblastoma and its proliferation aids in constituting proper treatment of choice at an early stage, preventing morbidity associated with extensive therapy. An attempt has been made to discuss the current concepts related to molecular and genetic changes that occur in ameloblastoma as these could affect treatment plan and prognosis. PMID:27721617

  8. Caracterização imuno-histoquímica de lesão híbrida de ameloblastoma desmoplásico e convencional "Hybrid" lesion of desmoplastic and conventional ameloblastoma: immunohistochemical aspects

    Directory of Open Access Journals (Sweden)

    Jean Nunes dos Santos

    2006-10-01

    Full Text Available O ameloblastoma é uma neoplasia odontogênica benigna comumente encontrada nos ossos maxilares. Histologicamente, mostra diversos padrões, incluindo a ameloblastoma plexiforme e folicular. Quando estes padrões histológicos coexistem com um ameloblastoma que exibe abundante desmoplasia, são então denominados de lesão ‘híbrida" de ameloblastoma desmoplásico e convencional. No presente trabalho, nos propomos a relatar um caso de lesão híbrida de ameloblastoma desmoplásico e convencional destacando os aspectos imuno-histoquímicos relativos a expressão das proteínas da matriz extracelular (tenascina, fibronectina e colágeno I.Ameloblastoma is a benign epithelial odontogenic tumor and is the most commonly encountered odontogenic tumor in the jaws. Histologically, ameloblastomas occur in different patterns, including plexiform pattern and follicular pattern. "Hybrid " lesion of ameloblastoma is a tumor variant in which histologically, areas of follicular or plexiform ameloblastoma coexist with characteristic areas of ameloblastoma exhibiting pronounced stromal desmoplasia (desmoplastic ameloblastoma. The purpose of this article is to present a case of "hybrid" lesion of desmoplastic ameloblastoma (AD and conventional, and investigate extracellular matrix proteins such as tenascin, fibronectin, and type I collagen.

  9. Ameloblastoma: a clinical and therapeutic analysis on six cases,

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    Frederico Barra de Moraes

    2014-06-01

    Full Text Available Ameloblastomas are odontogenic tumors that are locally invasive and slow-growing. Their etiology is still not well defined, but the forms of treatment have been widely discussed because of the possibility of tumor recurrence and postoperative complications. In this study, six patients who were diagnosed with ameloblastoma in the mandibular region and were treated in the Department of Orthopedics and Traumatology of Hospital das Clínicas, Federal University of Goiás, between 1958 and 1963, were evaluated. The radiological, clinical and therapeutic characteristics were evaluated. There was no predominance regarding gender in the sample studied. The symptoms most often presented by the patients were pain and tumor formation. The radiological characteristics with greatest incidence were multilocular lesions and the treatment used for all the patients was radical surgery. There was no recurrence over the minimum follow-up period of one year and six months.

  10. Molecular markers of tumor invasiveness in ameloblastoma: An update

    OpenAIRE

    Zhong, Yi; Guo, Wei; Wang, Li; Chen, Xinming

    2011-01-01

    The aim of the present article was to review the current new knowledge on the molecular markers of tumor invasion in ameloblastoma. In this review, tumor molecular markers were identified and allocated to the following six groups according to their functions: (I) Markers involved in extracellular matrix degradation, (II) Molecular markers involved in cell adhesion lost, (III) Molecular markers involved in bone remodeling, (IV) Cytokines involved in angiogenesis, (V) Molecular markers related ...

  11. Ameloblastoma of the jaws: Management and recurrence rate.

    Science.gov (United States)

    Laborde, A; Nicot, R; Wojcik, T; Ferri, J; Raoul, G

    2017-02-01

    Ameloblastoma is a rare, benign odontogenic tumour associated with a high recurrence rate. It accounts for 1% of all tumours of the jaws. The purpose of this study was to compare the ameloblastoma recurrence rate according to the type of treatment: radical or conservative. All patients with a diagnosis of ameloblastoma between 1991 and 2013 were retrospectively identified in order to extract topographic, radiological, and histological data and the type of treatment: conservative (marsupialization, enucleation, curettage) or radical (segmental resection) and to compare the recurrence rate according to the type of treatment. Twenty-seven patients were included, managed by conservative treatment (CT) in 22 cases and radical treatment (RT) in 14 cases. The recurrence rate was 90.9% in the CT group and 9.1% in the RT group (P=0.025) with a mean follow-up of 56.2 months. The recurrence rate after conservative treatment was higher than that after radical treatment. These results are similar to those reported in the literature. The choice of treatment must be adapted to the macroscopic and histological characteristics of each tumour and to the patient. Copyright © 2016. Published by Elsevier Masson SAS.

  12. Treatment of ameloblastoma and ameloblastic carcinoma with radiotherapy.

    Science.gov (United States)

    Kennedy, William R; Werning, John W; Kaye, Frederic J; Mendenhall, William M

    2016-10-01

    The purpose of this study is to report our institutional experience using radiotherapy in the treatment of ameloblastoma and ameloblastic carcinoma. Three patients with ameloblastoma and 3 patients with ameloblastic carcinoma were treated with radiotherapy alone (2 patients) or surgery and postoperative radiotherapy (4 patients) at the University of Florida between 1973 and 2007. Follow-up ranged from 4.0 to 13.1 years with a median of 7.8 years. Radiotherapy complications were scored using the Common Terminology Criteria for Adverse Events, version 4.0. Local control was achieved in 4 of the 6 patients. One patient treated with RT alone for an unresectable ameloblastoma developed a local recurrence and metastases in both the cervical lymph nodes and lungs, but had excellent response to dual BRAF/MEK inhibition with dabrafenib and trametinib. Another patient treated with surgery and postoperative radiotherapy for an ameloblastic carcinoma recurred locally without metastasis, but was not salvaged. No significant treatment-related complications were observed. For patients with local recurrence or inadequate margins after surgery, adjuvant radiotherapy provides the potential for disease control. In the setting of metastatic disease, targeted therapies may provide an additional opportunity for salvage.

  13. Intraosseous Ameloblastoma with a Prominent Extraosseous Component: Pitfalls in Diagnosis

    Science.gov (United States)

    Mishima, Kenji; Yamada, Hiroyuki; Kikuchi, Kentaro; Saito, Ichiro; Kusama, Kaoru

    2010-01-01

    For many years, gingival tumors of what appear to be peripherally located intraosseous ameloblastoma (IA) arising from the alveolar bone surface have often been confused with peripheral ameloblastoma (PA) causing resorption of the underlying bone. We analyzed a series of five cases of ameloblastoma that demonstrated a combined PA and IA architecture. The tumor commonly involved the anterior-premolar area, mostly in the maxilla and mainly in middle-aged men. The clinical presentation was an exophytic gingival mass inferior to which was a small bone defect. The predominant extraosseous component showed a papillary gross surface, reflecting the histologic proof of fusion between the submucosal tumor and the surface epithelium. In addition to the PA-like growth pattern, common to all was the presence of neoplastic destruction of the alveolar process, corresponding to an associated radiolucent lesion. This restrained component was acceptable as IA. In two cases, recurrence was observed deep in the alveolar bone with no involvement of the gingiva. These tumors appear to be IA that arose from the marginal alveolar bone and grew preferentially in the gingiva, forming a PA-like appearance. From diagnostic, therapeutic and prognostic points of view, this type of IA should not be confused with PA. PMID:20549402

  14. Role of Immunomarkers in the Clinicopathological Analysis of Unicystic Ameloblastoma

    Science.gov (United States)

    Sah, Parul; Kamath, Asha; Chandrashekar, Chetana; Carnelio, Sunitha

    2013-01-01

    Purpose. The clinical behavior of unicystic ameloblastoma varies according to its subtype. The assessment of its proliferative capacity, neovascularization, and invasiveness using relevant immunomarkers may aid in appropriate surgical therapeutic protocol. Methods. 18 cases of clinically and histologically confirmed unicystic ameloblastoma, categorized as luminal, intraluminal, or mural subtypes, were analyzed retrospectively. Immunomarkers such as Ki-67, CD34, MMP-2, and MMP-9 were studied to evaluate their behavior. Results. Labeling index of Ki-67 was 4.25% in the intraluminal subtype, compared with 2.14% in the luminal and 4.04% in the mural variant (P = 0.3). CD34 immunostaining was significantly higher in the mural variant (43 per high power field) than the other two subtypes (P = 0.04). MMP-2 and MMP-9 were strongly expressed in mural, moderately in intraluminal, and weakly to absent in luminal variant. Conclusions. High proliferative index, angiogenesis, and protease activity in the mural ameloblastoma, ascertained by the expression of these markers, confirm its aggressive phenotype. The intraluminal and luminal subtype exhibiting decreased expression are compatible with their indolent clinical behavior. PMID:24223460

  15. Caracterização imuno-histoquímica de lesão híbrida de ameloblastoma desmoplásico e convencional "Hybrid" lesion of desmoplastic and conventional ameloblastoma: immunohistochemical aspects

    OpenAIRE

    Jean Nunes dos Santos; Veronica Ferreira De Souza; Roberto Almeida Azevêdo; Viviane Almeida Sarmento; Lélia Batista Souza

    2006-01-01

    O ameloblastoma é uma neoplasia odontogênica benigna comumente encontrada nos ossos maxilares. Histologicamente, mostra diversos padrões, incluindo a ameloblastoma plexiforme e folicular. Quando estes padrões histológicos coexistem com um ameloblastoma que exibe abundante desmoplasia, são então denominados de lesão ‘híbrida" de ameloblastoma desmoplásico e convencional. No presente trabalho, nos propomos a relatar um caso de lesão híbrida de ameloblastoma desmoplásico e convencional destacand...

  16. Epidemiology of ameloblastomas of the jaws; a report from the Netherlands

    NARCIS (Netherlands)

    Oomens, M.A.E.M.; van der Waal, I.

    2014-01-01

    Objectives: To provide epidemiological data of ameloblastomas of the jaws in the Netherlands over a 25-year time period (1985-2010) and to compare these data with data from other parts of the world. Material and Methods: The data of all patients diagnosed with a primary ameloblastoma of the jaws in

  17. Immunohistochemical Detection of p75 Neurotrophin Receptor (p75-NTR) in Follicular and Plexiform Ameloblastoma

    Science.gov (United States)

    Madhavan, Nirmal Ramadas; Mohan, Sunil Paramel; Kumar, Srichinthu Kenniyan

    2016-01-01

    Introduction Ameloblastoma holds a unique position among benign tumours by its locally destructive and invasive nature. Recently improvised molecular techniques helped researchers to unravel the myth behind such biologic behaviour. Though interesting findings have been delivered, the rhythmic correlation regarding the exact mechanism still remains lacking. Neurotrophins and their receptor mediated pathways play a crucial role in survival, death and differentiation of many neuroectoderm derived cells. With this background, the study has been aimed to investigate the expression of p75-NTR (Neurotrophin Receptor) in follicular and plexiform ameloblastoma. Aim To analyze the immunohistochemical expression pattern of p75-NTR in ameloblastoma and to compare the immunohistochemical expression pattern of p75-NTR among the histological types of ameloblastoma, follicular and plexiform patterns. Materials and Methods Total 22 ameloblastomas (12 follicular, 10 plexiform) were immuno-stained with anti-human p75-NTR mouse IgG monoclonal antibody and the pattern of staining is statistically analyzed. Results Only 11 (10 follicular, 1 plexiform) out of 22 ameloblastomas showed immuno-reactivity to p75-NTR. In ameloblastoma, only the peripheral pre-ameloblast like tall columnar cells showed reactivity whereas the stellate reticulum-like cells were immuno-negative. The staining pattern was membranous in the immuno-reactive cells. The results were studied with the downstream pathways from the literature and a possible mechanism has been proposed. Conclusion The expression pattern of p75-NTR was found to be more in follicular ameloblastoma than plexiform. PMID:27656566

  18. Treatment of an extensive unicystic ameloblastoma in a 7-year-old child: the best approach?

    Science.gov (United States)

    de Paulo, L F B; Oliveira, M T F; Rodrigues, Á R; Zanetta-Barbosa, D

    2015-03-01

    Ameloblastoma is a benign, slow-growing neoplasm of the jaw that arises from odontogenic epithelium. We present the case of a 7-year-old girl with a unicystic ameloblastoma of the mandible, and describe our approach to treatment. Copyright © 2014 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  19. Clinicopathological analysis of histological variants of ameloblastoma in a suburban Nigerian population

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    Ndukwe Kizito C

    2006-11-01

    Full Text Available Abstract Background This study was carried out to establish the relative incidence and provide clinico-pathologic information on the various histological types of ameloblastoma seen at the Obafemi Awolowo University Teaching Hospital complex, Ile-Ife in order to provide a baseline data which will be of significance to the pathologist and clinician. Methods Clinico-pathologic data on a total of 77 histologically diagnosed cases of ameloblastoma archieved at the Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife over a 15 year period were obtained and analysed descriptively. Results Follicular ameloblastoma was the most common histological type (50 cases, 64.9%, followed by plexiform ameloblastoma (10 cases, 13.0%. 4 (5.2% cases of desmoplastic and 3 (3.9% cases of acanthomatous ameloblastoma were seen while the basal cell variant accounted for 2 (2.6% cases. Only 1 case of the unicystic type was seen. Some of the 77 cases presented as a mixture of two or more histological types. Ameloblastoma occurred over an age range of 11 to 70 years with a peak age incidence in the 3rd decade. Conclusion This study provides a baseline data on variants of ameloblastoma as obtained in a suburban Nigerian population. Since variants of ameloblastoma differ in biologic behaviour, the data collected in this study provides clinicopathologic information which is of significance to the pathologist and clinician.

  20. Current Concepts and Occurrence of Epithelial Odontogenic Tumors: I. Ameloblastoma and Adenomatoid Odontogenic Tumor

    Science.gov (United States)

    Kim, Yeon Sook

    2013-01-01

    Ameloblastomas and adenomatoid odontogenic tumors (AOTs) are common epithelial tumors of odontogenic origin. Ameloblastomas are clinico-pathologically classified into solid/multicystic, unicystic, desmoplastic, and peripheral types, and also divided into follicular, plexiform, acanthomatous, granular types, etc., based on their histological features. Craniopharyngiomas, derived from the remnants of Rathke's pouch or a misplaced enamel organ, are also comparable to the odontogenic tumors. The malignant transformation of ameloblastomas results in the formation of ameloblastic carcinomas and malignant ameloblastomas depending on cytological dysplasia and metastasis, respectively. AOTs are classified into follicular, extrafollicular, and peripheral types. Ameloblastomas are common, have an aggressive behavior and recurrent course, and are rarely metastatic, while AOTs are hamartomatous benign lesions derived from the complex system of the dental lamina or its remnants. With advances in the elucidation of molecular signaling mechanisms in cells, the cytodifferentiation of epithelial tumor cells in ameloblastomas and AOTs can be identified using different biomarkers. Therefore, it is suggested that comprehensive pathological observation including molecular genetic information can provide a more reliable differential diagnosis for the propagation and prognosis of ameloblastomas and AOTs. This study aimed to review the current concepts of ameloblastomas and AOTs and to discuss their clinico-pathological features relevant to tumorigenesis and prognosis. PMID:23837011

  1. Early dental epithelial transcription factors distinguish ameloblastoma from keratocystic odontogenic tumor.

    Science.gov (United States)

    Heikinheimo, K; Kurppa, K J; Laiho, A; Peltonen, S; Berdal, A; Bouattour, A; Ruhin, B; Catón, J; Thesleff, I; Leivo, I; Morgan, P R

    2015-01-01

    The aim of the study was to characterize the molecular relationship between ameloblastoma and keratocystic odontogenic tumor (KCOT) by means of a genome-wide expression analysis. Total RNA from 27 fresh tumor samples of 15 solid/multicystic intraosseous ameloblastomas and 12 sporadic KCOTs was hybridized on Affymetrix whole genome arrays. Hierarchical clustering separated ameloblastomas and KCOTs into 2 distinct groups. The gene set enrichment analysis based on 303 dental genes showed a similar separation of ameloblastomas and KCOTs. Early dental epithelial markers PITX2, MSX2, DLX2, RUNX1, and ISL1 were differentially overexpressed in ameloblastoma, indicating its dental identity. Also, PTHLH, a hormone involved in tooth eruption and invasive growth, was one of the most differentially upregulated genes in ameloblastoma. The most differentially overexpressed genes in KCOT were squamous epithelial differentiation markers SPRR1A, KRTDAP, and KRT4, as well as DSG1, a component of desmosomal cell-cell junctions. Additonally, the epithelial stem cell marker SOX2 was significantly upregulated in KCOT when compared with ameloblastoma. Taken together, the gene expression profile of ameloblastoma reflects differentiation from dental lamina toward the cap/bell stage of tooth development, as indicated by dental epithelium-specific transcription factors. In contrast, gene expression of KCOT indicates differentiation toward keratinocytes. © International & American Associations for Dental Research 2014.

  2. Clinicopathological analysis of histological variants of ameloblastoma in a suburban Nigerian population

    Science.gov (United States)

    Adebiyi, Kehinde E; Ugboko, Vincent I; Omoniyi-Esan, Ganiat O; Ndukwe, Kizito C; Oginni, Fadekemi O

    2006-01-01

    Background This study was carried out to establish the relative incidence and provide clinico-pathologic information on the various histological types of ameloblastoma seen at the Obafemi Awolowo University Teaching Hospital complex, Ile-Ife in order to provide a baseline data which will be of significance to the pathologist and clinician. Methods Clinico-pathologic data on a total of 77 histologically diagnosed cases of ameloblastoma archieved at the Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife over a 15 year period were obtained and analysed descriptively. Results Follicular ameloblastoma was the most common histological type (50 cases, 64.9%), followed by plexiform ameloblastoma (10 cases, 13.0%). 4 (5.2%) cases of desmoplastic and 3 (3.9%) cases of acanthomatous ameloblastoma were seen while the basal cell variant accounted for 2 (2.6%) cases. Only 1 case of the unicystic type was seen. Some of the 77 cases presented as a mixture of two or more histological types. Ameloblastoma occurred over an age range of 11 to 70 years with a peak age incidence in the 3rd decade. Conclusion This study provides a baseline data on variants of ameloblastoma as obtained in a suburban Nigerian population. Since variants of ameloblastoma differ in biologic behaviour, the data collected in this study provides clinicopathologic information which is of significance to the pathologist and clinician. PMID:17125498

  3. A study of ameloblastoma on the relationship between histopathologic patterns and radiographic characteristics

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    Choi, Hyun Bae; You, Dong Soo [Dept. of Oral Radiology, College of Dentistry, Seoul National University, Seoul (Korea, Republic of)

    1992-08-15

    The purpose of this study was to evaluate the correlationship between histopathologic types of ameloblastoma and their radiographic appearance.The materials for this study consisted of 106 patients diagnosed as ameloblastoma both radiographically and histologically. The obtained results were as follows: 1. The incidence of ameloblastoma in male (60 cases, 56.6%) was slightly higher than that in female (46 cases, 43.4%). The average age was estimated as 30.7 years with a range from 6 to 76 years. The second decade revealed the highest rate. 2. 106 ameloblastomas were histopathologically classified as 36 uicystic, 28 plexiform, 20 follicular, 14 acanthomatous, 7 granular cell, and 1 basalcell ameloblastoma. 3. Unilocular, soap-bubble appearance and scalloped margin were the radiographic appearances frequently seen in unicystic ameloblastoma . The predominant radiographic appearance of plexiform ameloblastoma showed unilocular radiolucency with scalloped margin. 4. 19.8%, 21 cases of ameloblastoma in this study showed containing tooth in their tumor mass by radiography. 5. Root resorption occurred in 37 cases (34.9%) and tooth displacement in 7 cases (6.6%). Root resorption and tooth displacement occurred in same patient were 24 cases (22.6%). 6. Recurrence occurred in 21.7% and average year between initial treatment and recurrence were 2 years.

  4. Basal Cell Ameloblastoma of Mandible: A Rare Case Report with Review

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    Hemant Shakya

    2013-01-01

    Full Text Available Ameloblastoma is a slow-growing benign neoplasm that has a strong tendency to local invasion and that can grow to be quite large without metastasizing. Rare examples of distant metastasis of an ameloblastoma in lungs or regional lymph nodes do exist. It has an aggressive and recurrent course and is rarely metastatic. Radiographically it shares common features with other lesions such as the giant cell tumor, aneurysmal bone cyst, and renal cell carcinoma metastasis; a definitive diagnosis can only be made with histopathology. Basal cell ameloblastoma is believed to be the rarest histologic subtype in which the tumor is composed of more primitive cells and has even fewer features of peripheral palisading. Till date, only few cases of basal cell ameloblastoma have been reported in the literature. Considering the rarity of the lesion, we report here an interesting and unique case of basal cell ameloblastoma of the mandible occurring in a very old patient.

  5. Basal cell ameloblastoma of mandible: a rare case report with review.

    Science.gov (United States)

    Shakya, Hemant; Khare, Vikram; Pardhe, Nilesh; Mathur, Ena; Chouhan, Mansi

    2013-01-01

    Ameloblastoma is a slow-growing benign neoplasm that has a strong tendency to local invasion and that can grow to be quite large without metastasizing. Rare examples of distant metastasis of an ameloblastoma in lungs or regional lymph nodes do exist. It has an aggressive and recurrent course and is rarely metastatic. Radiographically it shares common features with other lesions such as the giant cell tumor, aneurysmal bone cyst, and renal cell carcinoma metastasis; a definitive diagnosis can only be made with histopathology. Basal cell ameloblastoma is believed to be the rarest histologic subtype in which the tumor is composed of more primitive cells and has even fewer features of peripheral palisading. Till date, only few cases of basal cell ameloblastoma have been reported in the literature. Considering the rarity of the lesion, we report here an interesting and unique case of basal cell ameloblastoma of the mandible occurring in a very old patient.

  6. Assessment of ameloblastomas using MRI and dynamic contrast-enhanced MRI

    International Nuclear Information System (INIS)

    Asaumi, Jun-ichi; Hisatomi, Miki; Yanagi, Yoshinobu; Matsuzaki, Hidenobu; Choi, Yong Suk; Kawai, Noriko; Konouchi, Hironobu; Kishi, Kanji

    2005-01-01

    We retrospectively evaluated magnetic resonance images (MRI) and dynamic contrast-enhanced MRI (DCE-MRI) of ameloblastomas. MRI and DCE-MRI were performed for 10 ameloblastomas. We obtained the following results from the MRI and DCE-MRI. (a) Ameloblastomas can be divided into solid and cystic portions on the basis of MR signal intensities. (b) Ameloblastomas show a predilection for intermediate signal intensity on T1WI, high signal intensity on T2WI, and well enhancement in the solid portion; they also show a homogeneous intermediate signal intensity on T1WI and homogeneous high signal intensity on T2WI, and no enhancement in the cystic portion. (c) The mural nodule or thick wall can be detected in ameloblastomas lesions. (d) CI curves of ameloblastomas show two patterns: the first pattern increases, reaches a plateau at 100-300 s, then sustains the plateau or decreases gradually to 600-900 s, while the other increases relatively rapidly, reaches a plateau at 90-120 s, then decreases relatively rapidly to 300 s, and decreases gradually thereafter. There was no difference in the CI curve patterns among primary and recurrent cases, a case with glandular odontogenic tumor in ameloblastoma or among histopathological types such as plexiform, follicular, mixed, desmoplastic, and unicystic type

  7. Ameloblastoma RNA profiling uncovers a distinct non-coding RNA signature.

    Science.gov (United States)

    Davanian, Haleh; Balasiddaiah, Anangi; Heymann, Robert; Sundström, Magnus; Redenström, Poppy; Silfverberg, Mikael; Brodin, David; Sällberg, Matti; Lindskog, Sven; Kruger Weiner, Carina; Chen, Margaret

    2017-01-17

    Ameloblastoma of the jaws remains the top difficult to treat odontogenic tumour and has a high recurrence rate. New evidence suggests that non-coding RNAs (ncRNAs) play a critical role in tumourgenesis and prognosis of cancer. However, ameloblastoma ncRNA expression data is lacking. Here we present the first report of ameloblastoma ncRNA signatures. A total of 95 ameloblastoma cases and a global array transcriptome technology covering > 285.000 full-length transcripts were used in this two-step analysis. The analysis first identified in a test cohort 31 upregulated ameloblastoma-associated ncRNAs accompanied by signalling pathways of cancer, spliceosome, mRNA surveillance and Wnt. Further validation in an independent cohort points out the long non-coding (lncRNAs) and small nucleolar RNA (snoRNAs): LINC340, SNORD116-25, SNORA11, SNORA21, SNORA47 and SNORA65 as a distinct ncRNA signature of ameloblastoma. Importantly, the presence of these ncRNAs was independent of BRAF-V600E and SMO-L412F mutations, histology type or tumour location, but was positively correlated with the tumour size. Taken together, this study shows a systematic investigation of ncRNA expression of ameloblastoma, and illuminates new diagnostic and therapeutic targets for this invasive odontogenic tumour.

  8. BRAF inhibitor treatment of primary BRAF-mutant ameloblastoma with pathologic assessment of response.

    Science.gov (United States)

    Tan, Serena; Pollack, Jonathan R; Kaplan, Michael J; Colevas, A Dimitri; West, Robert B

    2016-07-01

    Molecular characterization of ameloblastoma has indicated a high frequency of driver mutations in BRAF and SMO. Preclinical data suggest that Food and Drug Administration-approved BRAF-targeted therapies may be immediately relevant for patients with ameloblastoma positive for the BRAF V600E mutation. A neoadjuvant treatment regime of dabrafenib was given to a patient with recurrent BRAF-mutant mandibular ameloblastoma. The patient subsequently underwent left mandible composite resection of the tumor and pathologic evaluation of treatment response. The ameloblastoma had a slow but dramatic response with >90% tumor volume reduction. The inner areas of the tumor underwent degeneration and squamous differentiation, and intact ameloblastoma was present in the outer areas associated with bone. Targeted neoadjuvant therapy for ameloblastoma may be useful in certain clinical settings of primary ameloblastoma. These might include tumors of advanced local stage when a neoadjuvant reduction could alter the extent of surgery and instances of local recurrence when surgical options are limited. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Suppression of local invasion of ameloblastoma by inhibition of matrix metalloproteinase-2 in vitro

    International Nuclear Information System (INIS)

    Wang, Anxun; Zhang, Bin; Huang, Hongzhang; Zhang, Leitao; Zeng, Donglin; Tao, Qian; Wang, Jianguang; Pan, Chaobin

    2008-01-01

    Ameloblastomas are odontogenic neoplasms characterized by local invasiveness. This study was conducted to address the role of matrix metalloproteinase-2 (MMP-2) in the invasiveness of ameloblastomas. Plasmids containing either MMP-2 siRNA or tissue inhibitor of metalloproteinase-2 (TIMP-2) cDNA were created and subsequently transfected into primary ameloblastoma cells. Zymography, RT-PCR, and Western blots were used to assess MMP-2 activity and expression of MMP-2 and TIMP-2, as well as protein levels. Primary cultures of ameloblastoma cells expressed cytokeratin (CK) 14 and 16, and MMP-2, but only weakly expressed CK18 and vimentin. MMP-2 mRNA and protein levels were significantly inhibited by RNA interference (P < 0.05). Both MMP-2 siRNA and TIMP-2 overexpression inhibited MMP-2 activity and the in vitro invasiveness of ameloblastoma. These results indicate that inhibition of MMP-2 activity suppresses the local invasiveness of ameloblastoma cells. This mechanism may serve as a novel therapeutic target in ameloblastomas pursuant to additional research

  10. BRAF-V600E expression correlates with ameloblastoma aggressiveness.

    Science.gov (United States)

    Fregnani, Eduardo R; Perez, Danyel E da Cruz; Paes de Almeida, Oslei; Fonseca, Felipe Paiva; Soares, Fernando A; Castro-Junior, Gilberto; Alves, Fábio A

    2017-02-01

    The aim of this study was to investigate whether the expression of BRAF-V600E determines an aggressive clinical and molecular presentation of ameloblastoma. Ninety-three cases of solid ameloblastomas were arranged in a 1.0-mm tissue microarray (TMA) block. Immunohistochemistry against a large panel of cytokeratins (CK), epidermal growth factor receptor (EGFR), parathyroid hormone-related peptide (PTHrP), syndecan-1, Ki67, p53 and BRAF-V600E were performed. Clinicopathological parameters, including sex, age, tumour size, tumour duration, tumour location, treatment, recurrences, radiographic pattern, vestibular/lingual and basal cortical plates disruption and follow-up data, were obtained from patients' medical records. Immunoexpression of BRAF-V600E was investigated in 73 cases that remained available in TMA sections. Our results indicated that 46.6% (34 cases) demonstrated cytoplasm positivity (six weak and 28 strong positivity). BRAF-V600E expression was associated significantly with the expression of CK8 (P = 0.00077), CK16 (P = 0.05), PTHrP (P = 0.0082) and p53 (P = 0.0087). Additionally, a significant association was seen with the presence of recurrences (P = 0.0008), multilocular radiographic appearance (P = 0.044) and disruption of basal bone cortical (P = 0.05). Univariate analysis showed that BRAF-positive cases (P = 0.001), EGFR-negative/weak positive cases (P = 0.03) and multilocular tumours (P = 0.04) had a significantly lower disease-free survival rate, but these parameters were not considered independent prognostic factors in the multivariate analysis (P > 0.05). Our findings suggest an association of BRAF-V600E with parameters of a more aggressive behaviour of ameloblastoma, supporting the future use of BRAF inhibitors for targeted therapy of this neoplasm. © 2016 John Wiley & Sons Ltd.

  11. Expression of molecules related to AKT pathway as putative regulators of ameloblastoma local invasiveness.

    Science.gov (United States)

    Cecim, Rodolpho L; Carmo, Hicso A F; Kataoka, Maria S S; Freitas, Vanessa M; de Melo Alves Júnior, Sérgio; Pedreira, Erick N; Jaeger, Ruy G; Pinheiro, Joao J V

    2014-02-01

    Ameloblastoma is an odontogenic neoplasm with local invasiveness and high recurrence. We previously suggested that growth factors, matrix metalloproteinases (MMPs), and TIMPs influence ameloblastoma invasiveness (Pathol. Res. Pract., 208, 2012, 225; Oral. Surg. Oral. Med. Oral. Pathol. Oral Radiol. Endod., 111, 2011, 474). Signals generated by this molecular network would be transduced by ERK 1/2 pathway (Oral. Surg. Oral. Med. Oral. Pathol. Oral Radiol. Endod., 111, 2011, 474). Others signaling pathways may influence ameloblastoma biology. Here, we studied expression of AKT and related molecules in ameloblastoma. Fourteen cases of solid/multicystic ameloblastomas were examined. Immunohistochemistry was carried out to detected AKT (phospho-AKT), NF-қB (phospho-NF-қB), β-catenin, cyclin-D1, and COX-2 in ameloblastoma samples. These molecules were evaluated in neoplastic cells and stroma. All proteins were detected in ameloblastoma. Expression of these markers was quantified and compared. Spearman's rank test was carried out to address positive correlations between proteins (P Ameloblastoma had a significant positive correlation of AKT (phospho-AKT) with β-catenin. β-catenin correlated with Cyclin-D1 and COX-2 in neoplastic cells. AKT (phospho-AKT) correlated with β-catenin; β-catenin with Cyclin-D1; AKT (phospho-AKT) with NF-қB (phospho-NF-қB); and NF-қB (phospho-NF-қB) with COX-2 in stromal cells. Results suggest that proteins studied are present and probably involved in a functional pathway in neoplastic cells and stroma and may therefore influence the local invasiveness of ameloblastoma. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  12. A comparative study of bone remodeling molecules expression in different types of jaw ameloblastoma.

    Science.gov (United States)

    Iakovou, Maria; Chrysomali, Evanthia; Piperi, Evangelia; Fanourakis, Galinos; Sklavounou, Alexandra; Vlachodimitropoulos, Dimitrios; Tseleni-Balafouta, Sophia

    2015-08-01

    Solid ameloblastoma demonstrates a more invasive behavior compared to unicystic. The follicular ameloblastoma is referred that may present a higher recurrence potential compared to the plexiform variant. In this study, the different ameloblastoma clinical types and histopathological variants were examined regarding the expression of bone remodeling-related molecules OPG, RANKL, and TRAIL. Immunostained sections of 29 solid and 11 unicystic ameloblastoma cases were semi-quantitatively evaluated and analyzed using Mann-Whitney or Kruskal-Wallis tests. Solid ameloblastoma showed a significantly increased OPG expression (P = 0.004) associated with the follicular (P ameloblastoma for differences by the histopathological pattern (no RANKL expression when plexiform pattern was seen compared to follicular). Comparison between the clinical types showed differences regarding the ratio of OPG/RANKL and TRAIL/RANKL expression. Higher OPG expression over RANKL was observed in 86.2% of the solid compared to 36.4% of the unicystic type. There was no difference in the ratio of TRAIL/RANKL expression in the unicystic, whereas 55.2% of the solid ameloblastomas showed a greater TRAIL expression over RANKL. Our results suggest OPG overexpression and RANKL underexpression in solid ameloblastoma; this may reflect a possible prevalence of the OPG/TRAIL over the OPG/RANKL signaling pathway, resulting in inactivation of TRAIL-induced apoptosis in ameloblastic cells. In unicystic ameloblastoma, the RANKL/OPG expression immunoprofile among histological variants is compatible with the reported biologic behavior. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  13. Recurrent ameloblastoma in temporal fossa: A diagnostic dilemma

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    Sagar S Vaishampayan

    2013-01-01

    Full Text Available Ameloblastoma is a unique, histologically benign but aggressive neoplasm of the jaws, arising from odontogenic epithelium with potency to cause extensive destruction of jaw bones and infiltration into the surrounding tissues. Recurrences are common after incomplete treatment. Recurrences can occur at difficult sites such as temporal and infratemporal fossa, orbit, anterior cranial base, paranasal sinuses etc. Fine needle aspiration cytology or core biopsy of these recurrent lesions may be misleading. Clinical course and radiological features help immensely in these situations. Good communication between surgeon, radiologist, and pathologist is of paramount importance.

  14. Reconstruction of palate with buccal fat pad secondary to resection of desmoplastic ameloblastoma

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    Bhimappa Mallappa Rudagi

    2015-04-01

    Full Text Available Desmoplastic ameloblastoma (DA is an unusual variant of ameloblastoma exhibiting important differences in the anatomical distribution, radiographic features and histologic appearance compared with the classic type of ameloblastoma. The purpose of this paper is to report a case of DA in the anterior left maxilla and to describe a simple method of reconstruction with the use of buccal fat pad (BFP. BFP is an excellent choice for reconstruction of small to medium sized defects. It should be manipulated gently and hemostasis should be achieved meticulously during this surgery. It should not be sutured under tension.

  15. Plexiform unicystic ameloblastoma-a case report and review of literature

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    Vikrant O. Kasat

    2014-01-01

    Full Text Available Ameloblastoma is categorized into four types; conventional solid (multicystic, unicystic, desmoplastic and peripheral varieties. Unicystic ameloblastoma (UA may originate de novo as a neoplasm or from the epithelium lining of an odontogenic cyst. When UA arising from cystic epithelium reveals a plexiform pattern of epithelial proliferation without early ameloblastic changes, it is called as "plexiform unicystic ameloblastoma" (PUA. The purpose of this article is to report a case of PUA in 22-year-old male and to discuss its pathogenesis, clinical features and management. Also, an effort is made to review early literature on this entity.

  16. Recurrent unicystic mural type ameloblastoma in a 9-year-old boy, 8 years follow-up

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    Burcu Sengüven

    2013-01-01

    Full Text Available Unicystic ameloblastoma is not a rare odontogenic tumor in the pediatric population. A significant care should be given to unicystic ameloblastoma if it has mural invasions due to its local aggressiveness, high recurrence rates and radical management options as in conventional ameloblastoma. Fine needle aspiration (FNA cytology is a rapid, non-traumatic diagnostic method that provides a required attention prior to surgery. We present an excisionsl biopsy proved FNA diagnosed mural type unicystic ameloblastoma in a 9-year-old child recurred as a solid ameloblastoma after 8 years. When distinctive features of ameloblastoma are known, an accurate diagnosis can be made by FNA cytology, in combination with clinicoradiological findings. This method gives benefit to the patients especially the younger ones both for the pre-operative surgical planning and the post-operative follow-up.

  17. Desmoplastic ameloblastoma in the anterior mandible crossing the midline: A rare variant

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    Appaji Athota

    2009-01-01

    Full Text Available Desmoplastic ameloblastoma (DA is a rare variant of ameloblastoma. One hundred and forty-five cases of desmoplastic ameloblastoma have been reported till 2007 in the literature. This article presents a rare case of desmoplastic ameloblastoma in the parasymphysial region. Review of the literature has revealed that the tumor most commonly occurs in the third to fifth decade of life, with a male predilection of 3:2. The anterior region of the maxilla is the part that is most commonly found to be affected. The radiological features reveal a mixed radiolucent-radiopaque lesion with poorly defined to well-defined borders. However, our case differed from the reviewed cases, as it was found to be with well-defined borders. The histological findings of the case were consistent with the histological appearance of the reviewed cases and showed extensive stromal desmoplasia and small tumor nests of odontogenic epithelium scattered in the stroma.

  18. Maxillary plexiform ameloblastoma showing basaloid differentiation: Report of a rare case with review of literature.

    Science.gov (United States)

    Ghattamaneni, Sravani; Guttikonda, Venkateswara Rao; Kumari, M Geetha; Kumar, D Rakesh

    2015-01-01

    Ameloblastoma is a benign, locally aggressive tumor originating from the odontogenic epithelium. It manifests as a slow growing swelling, causing expansion of the jaw bones. Radiologically, it presents as a unilocular or multilocular radiolucency exhibiting a characteristic soap bubble or honeycomb appearance. Ameloblastoma exhibits several histologic patterns of which basal cell variant is a rare entity. The present case report is that of a maxillary ameloblastoma exhibiting a basaloid differentiation that may put one in the mind of a basaloid squamous cell carcinoma or a basal cell carcinoma. Confirmation of such rare variants should be done not only based on histopathology but with the help of supplemental immunohistochemical analysis. The present case report helps in exposing a rare variant of ameloblastoma and emphasizes the role of advanced diagnostic aids such as immunohistochemistry in establishing the diagnosis.

  19. Ameloblastoma of the Sinonasal Tract: Report of a Case with Clinicopathologic Considerations

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    Maria Grazia Tranchina

    2012-01-01

    Full Text Available Ameloblastomas are locally aggressive jaw tumours with a high propensity for recurrence and are believed to arise from remnants of dental lamina or odontogenic epithelium. Extragnathic ameloblastomas are unusual, and primary sinonasal tract origin is very uncommon with few cases reported in the literature. We herein report a case of primary sinonasal ameloblastoma presented in a 74-year-old male with nasal obstruction, rhinorrhoea, and sinusitis. Nasal endoscopy showed the right nasal cavity completely obstructed by a polypoid lesion attached to the lateral nasal wall. A preoperative CT scan was performed showing a solid lesion, measuring 2 cm in the maximum diameter, extending from the nasopharynx area with obstruction of the ostiomeatal unit and sphenoethmoidal recess into the lateral pharyngeal space, laterally to the parotid, without continuity with maxillary alveola and antrum. The tumour was completely excised endoscopically, and a final diagnosis of ameloblastoma was rendered. At the 12-month followup, there was no evidence of recurrence.

  20. Is Conservative Surgical Treatment Sufficient to Treat Unicystic Mural Ameloblastoma in Infant?

    Science.gov (United States)

    Acar, Ahmet Hüseyin; Yolcu, Ümit; Erdem, Necip Fazil; Asutay, Fatih

    2015-06-01

    Ameloblastoma, a benign neoplasm derived from odontogenic epithelium, is an aggressive and locally invasive tumor. It represents 11% of all odontogenic tumors and 1% of all oral odontogenic epithelial tumors. In this case report, a 20-month-old boy was referred to our clinic with complaint of collapse in his symphysis region of the mandible. Radiographic examination revealed unilocular radiolucency in this region. The lesion was enucleated with 1 tooth germ under general anesthesia and diagnosed as mural unicystic ameloblastoma by histopathologic examination. After the surgery, complete healing was obtained clinically and radiographically. No sign of recurrence has been seen during the follow-up period of 4.5 years. To our knowledge, this was the second youngest case of ameloblastoma in the English literature. However, it is the youngest case of ameloblastoma that occurred in an infant boy.

  1. Expression and mechanism of regulation of PP2A/Pr65 in ameloblastoma.

    Science.gov (United States)

    Gao, Xiuqiu; Wang, Guannan; Zhang, Yun-Kai; Zhong, Ming

    2014-06-01

    This study aimed to investigate the expression of PP2A/PR65 protein in ameloblastoma and the molecular mechanisms underlying the regulation of PP2A/PR65. The association between PP2A/PR65 and the clinicopathological characteristics of tumor specimens in ameloblastoma were to provide a theoretical basis for the diagnosis, therapy and prognosis of ameloblastoma. Streptavidin-peroxidase (S-P) immunohistochemical staining was used to detect PP2A/Pr65 expression changes in a total of 68 cases of ameloblastoma, six ameloblastic carcinomas, 21 squamous cell carcinomas and seven normal oral mucosas. Western blot was used to analyze PP2A/PR65 protein expression in 15 cases of ameloblastoma and three cases of normal oral mucosa. Of the 68 cases analyzed, four cases were negative, 25 cases were weakly positive, 20 cases were moderately positive and 19 cases were strongly positive. In six cases of ameloblastic carcinoma, three cases were weak positive, one case was positive, two cases were strongly positive and none were negative. In 21 cases of squamous cell carcinomas, three cases were negative, 17 cases were weakly positive, one case was moderately positive and none were strongly positive. Western blot analysis showed that, PP2A/Pr65 protein expression was lower in ameloblastoma tissue compared with normal oral mucosa. Reduced expression of PP2A/PR65 in ameloblastoma compared with normal oral mucosa indicates that PP2A/PR65 is involved in the occurrence and development of ameloblastoma. Copyright © 2013 Royal College of Surgeons of Edinburgh (Scottish charity number SC005317) and Royal College of Surgeons in Ireland. Published by Elsevier Ltd. All rights reserved.

  2. An unusual case of bilateral unicystic ameloblastoma in third molar region

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    Vikram Deshmukh

    2014-01-01

    Full Text Available Unicystic ameloblastoma (UA is less encountered variant which is believed to be less aggressive. A rare case of bilateral unicystic mural ameloblastoma in a 24 year old pregnant female is presented which was treated by en-block resection with preservation of the lower border of the mandible. The rationale for this treatment modalitiy and other treatment options for this condition including post resection reconstruction have been discussed in this article.

  3. Hybrid desmoplastic ameloblastoma: A case report of rare entity and review of literature

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    Priya Shirish Joshi

    2014-01-01

    Full Text Available Ameloblastomas are one of the common odontogenic tumors of the oral cavity. They usually present with unilocular or multilocular radiolucency associated with or without impacted tooth. Among the variants of ameloblastomas, desmoplastic ameloblastoma (DA casts a mixed radiolucent/radiopaque shadow on X-ray and radiograph seldom suggests a diagnosis of ameloblastoma. DA is a rare entity that exhibits important differences in anatomic distribution, histologic appearance, radiographic findings when compared to other variants of ameloblastoma. This fact has been considered by World Health Organization (WHO and now it has been included as a separate entity in WHO classification (2005 of odontogenic tumors. About 145 cases of DA have been reported worldwide, but cases reported in India are very few. We report a case of a hybrid variety of DA in a female patient in the anterior maxillary region between 12 and 13 as painless hard swelling, showing mixed radiolucent/radiopaque shadow on radiographic examination. Histopathology revealed odontogenic epithelium in the form of follicles, typical of solid/multicystic ameloblastoma, with cystic degeneration and squamous metaplasia at places and elsewhere there were odontogenic islands compressed by dense fibrocellular stroma suggestive of desmoplasia. Also seen was osseous metaplasia. We have also done a detailed review of literature concerning the presentation of DA.

  4. Potential involvement of chondroitin sulfate A in the pathogenesis of ameloblastoma.

    Science.gov (United States)

    Li, Xiangjun; Kurita, Hiroshi; Xiao, Tiepeng; Iijima, Kyou; Kurashina, Kenji; Nakayama, Jun

    2017-06-01

    Ameloblastoma is classified as a benign odontogenic tumor characterized by locally invasive behavior and high risk of recurrence. Here, we evaluate a potential role for glycosaminoglycan, a structural component of cell membranes and extracellular matrix, in ameloblstoma pathogenesis. We subjected formalin-fixed, paraffin-embedded tissue sections of 34 cases of ameloblastoma, 10 of odontogenic keratocyst, and 17 of dentigerous cyst to immunohistochemistry using monoclonal antibodies recognizing chondroitin sulfate A (CS-A), heparan sulfate (HS), and keratan sulfate (KS). Expression levels of CS-A in epithelial component and stroma of ameloblastoma were significantly higher than those in odontogenic keratocyst and dentigerous cyst. Moreover, CS-A in ameloblastoma was more strongly expressed in stellate reticulum-like cells than in amelobast-like cells with statistical significance. On the other hand, expression levels of HS and KS in epithelial component and stroma of ameloblastoma were lower compared with CS-A. These results overall reveal that among these odontogenic lesions, CS-A is preferentially expessed in ameloblastoma, suggesting potential pathogenetic role probably in cytodifferention of tumor cells to stellate reticulum-like cells. Copyright © 2017 Elsevier GmbH. All rights reserved.

  5. A novel marker of ameloblastoma and systematic review of immunohistochemical findings.

    Science.gov (United States)

    Khalele, Bacem A E O; Al-Shiaty, Rami A

    2016-06-01

    This study aims at investigating the pathogenesis and oncogenesis of ameloblastoma. Being the commonest odontogenic tumor with idiopathic nature, ameloblastoma poses a fierce controversy about its oncogenesis. Immunohistochemical markers, over years, have highlighted specific pathways which are inherently undertaken in the tumorigenic process of ameloblastoma. Besides the recently pronounced clue of BRAF V600E mutant gene, this study introduces a new marker with its outstanding impact on our contemporary knowledge about ameloblastoma. Extrapolating from the systematic review of medical literature and recruiting a novel immunohistochemical marker, ameloblastoma enacts a new scenario supporting the approved involvement of MAPK by overexpressing WT1 a total of 37 archival cases, regardless of the histological variant in study. There evinces a significant contribution of Wilm's tumor gene, as an oncogene rather than a suppressor gene, to the pathogenesis of the ameloblastomatous tumorigenesis. Moreover, no ameloblastomatous histological phenotype has established, given the literature underpinned, a concrete impact on the clinical behavior. Immunohistochemical research papers which investigated tumorigenesis - although they do not quantitatively measure much- had the most significant impact on the diagnostic and prognostic levels. WT1 may play, therefore, a remarkable role in the oncogenesis of ameloblastoma. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. Conservative approach: using decompression procedure for management of a large unicystic ameloblastoma of the mandible.

    Science.gov (United States)

    Xavier, Samuel Porfirio; de Mello-Filho, Francisco Veríssimo; Rodrigues, Willian Caetano; Sonoda, Celso Koogi; de Melo, Willian Morais

    2014-05-01

    Ameloblastoma is a relatively uncommon benign odontogenic tumor, which is locally aggressive and has a high tendency to recur, despite its benign histopathologic features. This pathology can be classified into 4 groups: unicystic, solid or multicystic, peripheral, and malignant. There are 3 variants of unicystic ameloblastoma, as luminal, intraluminal, and mural. Therefore, in mural ameloblastoma, the fibrous wall of the cyst is infiltrated with tumor nodules, and for this reason it is considered the most aggressive variant of unicystic ameloblastomas. Various treatment techniques for ameloblastomas have been proposed, which include decompression, enucleation/curettage, sclerotizing solution, cryosurgery, marginal resection, and aggressive resection. Literature shows treatment of this lesion continues to be a subject of intense interest and some controversy. Thus, the authors aimed to describe a case of a mural unicystic ameloblastoma of follicular subtype in a 19-year-old subject who was successfully treated using conservative approaches, as decompression. The patient has been followed up for 3 years, and has remained clinically and radiographically disease-free.

  7. Anti-apoptotic role of the sonic hedgehog signaling pathway in the proliferation of ameloblastoma.

    Science.gov (United States)

    Kanda, Shiori; Mitsuyasu, Takeshi; Nakao, Yu; Kawano, Shintaro; Goto, Yuichi; Matsubara, Ryota; Nakamura, Seiji

    2013-09-01

    Sonic hedgehog (SHH) signaling pathway is crucial to growth and patterning during organogenesis. Aberrant activation of the SHH signaling pathway can result in tumor formation. We examined the expression of SHH signaling molecules and investigated the involvement of the SHH pathway in the proliferation of ameloblastoma, the most common benign tumor of the jaws. We used immunohistochemistry on ameloblastoma specimens and immunocytochemistry and reverse transcription-PCR on the ameloblastoma cell line AM-1. We also used the inhibitors of SHH signaling, SHH neutralizing antibody and cyclopamine, to assess the effects of SHH on the proliferation of AM-1 cells. We detected expression of SHH, patched, GLI1, GLI2 and GLI3 in the ameloblastoma specimens and AM-1 cells. The proliferation of these cells was significantly inhibited in the presence of SHH neutralizing antibody or cyclopamine; this was confirmed by BrdU incorporation assays. Furthermore, in the presence of SHH neutralizing antibody, nuclear translocation of GLI1 and GLI2 was abolished, apoptosis was induced, BCL-2 expression decreased and BAX expression increased. Our results suggest that the SHH signaling pathway is constitutively active in ameloblastoma and plays an anti-apoptotic role in the proliferation of ameloblastoma cells through autocrine loop stimulation.

  8. Study of Ki67 and CD10 expression as predictive factors of recurrence of ameloblastoma.

    Science.gov (United States)

    Ahlem, B; Wided, A; Amani, L; Nadia, Z; Amira, A; Faten, F

    2015-11-01

    Ameloblastoma is a rare, benign, purely epithelial odontogenic tumour, characterized by a high potential for local invasion and recurrence. To study the epidemiological and histological characteristics of ameloblastoma. To study Ki67 and CD10 immunostaining in ameloblastoma and to investigate a possible correlation between these two markers and recurrence of this tumour. An immunohistochemical study using Ki67 and CD10 monoclonal antibodies was performed on 37 paraffin blocks obtained from the Charles-Nicolle hospital pathology department in Tunis over a 9-year period (2004-2012). Statistical analysis was performed with Statistical Package for Social Sciences (SPSS) software version 15.1. This series of 37 cases comprised 21 males and 16 females (sex ratio: 1.3) with a mean age of 39 years (range: 7 to 70 years), corresponding to 36 cases of intraosseous ameloblastoma and one case of gingival ameloblastoma. Thirty-two cases were polycystic and 5 cases were unicystic. Eighteen cases of local recurrence were observed. No correlation was demonstrated between recurrence and the various clinical and histological parameters and treatment modalities. However, a significant correlation was demonstrated between recurrence and Ki67 and CD10 expression (P=0.000 and 0.002, respectively). The Ki67 proliferation index and stromal CD10 expression can be considered to be predictive factors of ameloblastoma recurrence. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  9. Invadopodia proteins, cortactin, N-WASP and WIP differentially promote local invasiveness in ameloblastoma.

    Science.gov (United States)

    Siar, Chong Huat; Rahman, Zainal Ariff Bin Abdul; Tsujigiwa, Hidetsugu; Mohamed Om Alblazi, Kamila; Nagatsuka, Hitoshi; Ng, Kok Han

    2016-09-01

    Cell migration and invasion through interstitial tissues are dependent upon several specialized characteristics of the migratory cell notably generation of proteolytic membranous protrusions or invadopodia. Ameloblastoma is a benign odontogenic epithelial neoplasm with a locally infiltrative behaviour. Cortactin and MMT1-MMP are two invadopodia proteins implicated in its local invasiveness. Other invadopodia regulators, namely N-WASP, WIP and Src kinase remain unclarified. This study addresses their roles in ameloblastoma. Eighty-seven paraffin-embedded ameloblastoma cases (20 unicystic, 47 solid/multicystic, 3 desmoplastic and 17 recurrent) were subjected to immunohistochemistry for expression of cortactin, N-WASP, WIP, Src kinase and F-actin, and findings correlated with clinicopathological parameters. Invadopodia proteins (except Src kinase) and F-actin were widely detected in ameloblastoma (cortactin: n = 73/87, 83.9%; N-WASP: n = 59/87; 67.8%; WIP: n = 77/87; 88.5%; and F-actin: n = 87/87, 100%). Protein localization was mainly cytoplasmic and/or membranous, and occasionally nuclear for F-actin. Cortactin, which functions as an actin-scaffolding protein, demonstrated significantly higher expression levels within ameloblastoma tumoral epithelium than in stroma (P ameloblastoma is dependent upon the migratory potential of its tumour cells as defined by their distribution of cortactin, N-WASP and WIP in correlation with F-actin cytoskeletal dynamics. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  10. An unusual case of unicystic intramural ameloblastoma and review of the literature

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    Massimiliano Ricci

    2012-01-01

    Full Text Available Ameloblastoma is the most common tumor of odontogenic origin. There are various types of this tumor and confusion still exists among the clinicians about the correct classification. Multicystic ameloblastoma is the most frequent subtype while unicystic ameloblastoma can be considered as a variant of the solid or multycistic. This subtype is considered as a less aggressive tumor with a variable recurrence rate. However, its frequency is often underestimated. The aim of this article is reviewing the recent literature about unicystic ameloblastoma using our unusual clinical case as a starting point to illustrate this discussion. A 30-year-old man who had been complaining of slight pain in the premolar and molar area of the left side of mandible had a check up at our department. X-rays revealed a unilocular radiotrasparency with radiopaque margins. The first histological diagnosis was an odontogenic cyst. Successive histological evaluations revealed that ameloblastic epithelial islands were present in lassus connective tissue. We think that our case report provides new insights into the approach to the ameloblastoma diagnosis. We agree with authors who have pointed out that a single small biopsy may often be inadequate for the correct diagnosis of amelobastoma. Moreover, in the light of our experience, it should be kept in mind that ameloblastomas may have sometimes unusual presentations and this fact should induce surgeons and pathologists to consider carefully each lesion.

  11. Regulation of IL-6 and IL-8 production by reciprocal cell-to-cell interactions between tumor cells and stromal fibroblasts through IL-1α in ameloblastoma

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    Fuchigami, Takao [Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan); Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan); Kibe, Toshiro [Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan); Koyama, Hirofumi; Kishida, Shosei; Iijima, Mikio [Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan); Nishizawa, Yoshiaki [Kagoshima University Faculty of Medicine, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan); Hijioka, Hiroshi; Fujii, Tomomi [Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan); Ueda, Masahiro [Natural Science Centre for Research and Education, Kagoshima University, 1-21-24 Koorimoto, Kagoshima 890-8580 (Japan); Nakamura, Norifumi [Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan); Kiyono, Tohru [Department of Virology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuouku, Tokyo 104-0045 (Japan); Kishida, Michiko, E-mail: kmichiko@m2.kufm.kagoshima-u.ac.jp [Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544 (Japan)

    2014-09-05

    Highlights: • We studied the interaction between tumor cells and fibroblasts in ameloblastoma. • AM-3 ameloblastoma cells secreted significantly high IL-1α levels. • IL-1α derived from AM-3 cells promoted IL-6 and IL-8 secretion of fibroblasts. • IL-6 and IL-8 activated the cellular motility and proliferation of AM-3 cells. - Abstract: Ameloblastoma is an odontogenic benign tumor that occurs in the jawbone, which invades bone and reoccurs locally. This tumor is treated by wide surgical excision and causes various problems, including changes in facial countenance and mastication disorders. Ameloblastomas have abundant tumor stroma, including fibroblasts and immune cells. Although cell-to-cell interactions are considered to be involved in the pathogenesis of many diseases, intercellular communications in ameloblastoma have not been fully investigated. In this study, we examined interactions between tumor cells and stromal fibroblasts via soluble factors in ameloblastoma. We used a human ameloblastoma cell line (AM-3 ameloblastoma cells), human fibroblasts (HFF-2 fibroblasts), and primary-cultured fibroblasts from human ameloblastoma tissues, and analyzed the effect of ameloblastoma-associated cell-to-cell communications on gene expression, cytokine secretion, cellular motility and proliferation. AM-3 ameloblastoma cells secreted higher levels of interleukin (IL)-1α than HFF-2 fibroblasts. Treatment with conditioned medium from AM-3 ameloblastoma cells upregulated gene expression and secretion of IL-6 and IL-8 of HFF-2 fibroblasts and primary-cultured fibroblast cells from ameloblastoma tissues. The AM3-stimulated production of IL-6 and IL-8 in fibroblasts was neutralized by pretreatment of AM-3 cells with anti-IL-1α antibody and IL-1 receptor antagonist. Reciprocally, cellular motility of AM-3 ameloblastoma cells was stimulated by HFF-2 fibroblasts in IL-6 and IL-8 dependent manner. In conclusion, ameloblastoma cells and stromal fibroblasts behave

  12. Maxillary Ameloblastoma with Orbital Involvement: An Institutional Experience and Literature Review.

    Science.gov (United States)

    Milman, Tatyana; Lee, Vivian; LiVolsi, Virginia

    To describe 8 patients with orbital involvement by ameloblastoma and to review the literature on this topic. The electronic medical records and pathology databases of the Hospital of the University of Pennsylvania were searched to identify all patients with histopathologically confirmed ameloblastoma diagnosed between 1990 and 2015. PubMed database was searched for all well-documented cases of maxillary ameloblastoma and ameloblastic carcinoma ex-ameloblastoma with orbital involvement published in the English literature. The information collected on the compiled 23 patients included age, sex, clinical presentation, imaging findings, management, tumor histopathologic features, and follow up. Review of medical records identified 8 patients with orbital involvement by ameloblastoma. Literature search yielded 15 patients with well-documented orbital involvement by ameloblastoma. Most tumors occurred in men (19 of 23, M:F = 4-5:1) with an average age of 56 years. The overall rates of recurrence, visual compromise, death, and confirmed disease-related mortality were 70% (16/23), 26% (6/23), 39% (9/23), and 22% (5/23), respectively. The initial surgical approach correlated with prognosis. The rates of recurrence, orbital exenteration, and mortality in the cohort managed with conservative surgery or partial maxillectomy were 57% (8/14), 29% (4/14), and 50% (7/14), respectively. In contrast, the patients initially managed with a radical resection had substantially lower frequencies of tumor recurrence (2/7, 29%), exenteration (1/7, 14%), and death (1/7, 14%). Malignant transformation to ameloblastic carcinoma occurred in the setting of recurrent disease in 3 patients and in 1 patient with prolonged duration of symptoms, suggestive of a long-standing tumor. Maxillary ameloblastoma can rarely involve the orbit, leading to significant ocular morbidity and occasional mortality. Prompt radical resection of the tumor has the potential to decrease the likelihood of recurrence and

  13. Immunohistochemicai study of Ki- 67 expression in unicystic Ameloblastoma and Dentigerous cyst

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    Eslami M.

    2004-06-01

    Full Text Available Statement of Problem: Differentiation of dentigerous cyst from unicystic ameloblastoma, discovering any initial ameloblastic changes in lining epithelium of dentigerous cyst at early stage, and differentiation between hyperplastic odontogenic epithelium in fibrous capsule of dentigerous cyst from ameloblastic proliferation, need to an accurate and reliable technique."nPurpose: The aim of this study was to determine and compare Ki-67 immunoreactivity in various locations of the epithelium of Dentigerous cyst and Unicystic Ameloblastoma."nMaterials and Methods: In this historical Cohort study, 15 cases of dentigerous cyst and 9 cases of unicystic ameloblastoma were selected. Immunohistochemistry staining was performed by M1B-1 (murine monoclonal antibody against Ki-67. The stained nucleous were counted in basal and suprabasal layer of lining epithelium of both lesions in 3000 epithelial cells. Finally, the percentage of positive cells (presented as labeling index was calculated, t- student test was used to analyze the related data."nResults: Ki-67 (LI in basal layer of Dentigerous cyst (2.59±1.66 and Unicystic Ameloblastoma (3.76±79 had no significant differences, but Ki-67 (LI in suprabasal layer of unicystic ameloblastoma (2.15±0.69 was significantly higher than dentigerous cyst (0.77±0.55 P=0.003."nThe difference between the average numbers of positive cells for Ki-67 (LI in these two lesions was statistically significant (P<0.05 and it was higher in Unicystic Ameloblastoma than Dentigerous cyst."nConclusion: Based on the findings of this study, it is suggested that Ki-67 (LI in suprabasal layer or throughout the epithelium can be considered as a useful marker for differential diagnosis between dentigerous cyst and unicystic ameloblastoma.

  14. Desmoplastic ameloblastoma mimicking a periapical lesion = Ameloblastoma desmoplásico mimetizando lesão periapical

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    Santos, Esaú Pinheiro dos

    2010-01-01

    Full Text Available Ameloblastoma desmoplásico (AD é uma rara variante do ameloblastoma, com características clínicas, imaginológicas e histopatológicas específicas. O perfil biológico deste tumor não é totalmente compreendido, devido ao número limitado de casos notificados. O objetivo deste trabalho é relatar um caso de AD simulando uma lesão periapical. Este tumor apareceu na região anterior da maxila esquerda numa paciente do gênero feminino de 24 anos de idade, entre o incisivo lateral esquerdo e o canino. A lesão era indolor e apresentava apenas um ligeiro inchaço da mucosa do palato duro. Radiograficamente, uma área radiolúcida bem delimitada que promovia a divergência radicular foi observada. O incisivo lateral respondeu negativamente aos testes pulpares. Diagnóstico provisório de cisto radicular versus cisto fissural foi estabelecido e enucleação cirúrgica seguida de curetagem foi realizada. O exame histológico das amostras revelou a proliferação dos ninhos e cordões de epitélio odontogênico comprimido imersos em um estroma densamente colagenizado, associada a um trabeculado ósseo levemente irregular. Estas características eram consistentes com AD. A necrose pulpar do incisivo e o aparecimento radiográfico atípico, deixava a doença em absconso. Apesar das altas taxas de recorrência, nenhum sinal clínico ou radiográfico de recidiva do tumor foi detectado um ano mais tarde

  15. A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis.

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    Kibe, Toshiro; Fuchigami, Takao; Kishida, Michiko; Iijima, Mikio; Ishihata, Kiyohide; Hijioka, Hiroshi; Miyawaki, Akihiko; Semba, Ichiro; Nakamura, Norifumi; Kiyono, Tohru; Kishida, Shosei

    2013-06-01

    Ameloblastoma has a high risk of bone invasion and local recurrence. However, the mechanisms of bone invasion in ameloblastoma remain unclear. In this study, we established an experimental model for matrix metalloproteinase (MMP) induction and osteoclastogenesis using ameloblastoma-derived cells. We established an ameloblastoma-derived cell line without viral genes and analyzed the expression of all Wnt and Frizzled members and MMPs by real-time reverse transcription-polymerase chain reaction, and analyzed the activity of MMP-2 and MMP-9 by the in-gel-gelatinase assay. AM-3, newly established ameloblastoma-derived cells retained the morphology of primary-cultured ameloblastoma cells. AM-3 cells overexpressed the messenger RNA of Wnt-5a, Frizzled-2, MMP-2, and MMP-9 and showed the potential of osteoclastogenesis. In addition, Wnt-3a-treatment induced expression and activation of MMP-9 in AM-3 cells. Our study suggests that AM-3 cells retained the characteristics of ameloblastoma, without acquiring typical features of cancer cells. Furthermore, Wnt signaling induced MMP-9 in ameloblastoma cells. Copyright © 2013 Elsevier Inc. All rights reserved.

  16. Hybrid ameloblastoma and adenomatoid odontogenic tumor: report of a case and review of hybrid variations in the literature.

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    Yamazaki, Manabu; Maruyama, Satoshi; Abé, Tatsuya; Babkair, Hamzah; Fujita, Hajime; Takagi, Ritsuo; Koyama, Jun-Ichi; Hayashi, Takafumi; Cheng, Jun; Saku, Takashi

    2014-07-01

    Hybrid odontogenic tumors including 2 or more different histologic types have been documented, but their occurrences are not very common. We present a case of hybrid odontogenic tumor composed of ameloblastoma and adenomatoid odontogenic tumor (AOT) arising in the mandibular molar region of a 31-year-old Japanese woman who had a history of familial adenomatous polyposis. The tumor, measuring 10 mm in diameter, was surgically removed from the alveolar bone. Histopathologically, the tumor consisted of both follicular and plexiform types of ameloblastoma in which multiple and smaller foci of AOT were intermingled. There have been 3 reported cases of hybrid ameloblastoma and AOT, all of which presented unicystic types as ameloblastoma components. This, however, is the first report of a hybrid tumor containing an authentic solid-type ameloblastoma compartment and an AOT compartment in a patient with a background of familial adenomatous polyposis. Copyright © 2014 Elsevier Inc. All rights reserved.

  17. Squamous cell carcinoma (Marjolin's ulcer in an orocutaneous fistula of a large mandibular ameloblastoma: a case report

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    Nthumba Peter M

    2011-08-01

    Full Text Available Abstract Introduction Ameloblastomas are rare lesions constituting 1% of all jaw tumors. Oral squamous cell carcinomas are common lesions; these constitute about 90% of all oral cancers. Concurrent tumors consisting of ameloblastoma and squamous cell carcinoma are extremely rare. Case presentation This case report describes a 35-year-old African man who presented with a large mandibular tumor with an orocutaneous fistula that was found to be an ameloblastoma on histopathological examination, with concurrent squamous cell carcinoma histology within the fistula. This presentation was consistent with a Marjolin's ulcer within an ameloblastoma. Conclusion Ameloblastomas and Marjolin's ulcers require different management strategies. Careful histopathological examination of surgical specimens is key to patient outcome, as treatment of these patients depends on an accurate diagnosis.

  18. Differential expression of transcription factors Snail, Slug, SIP1, and Twist in ameloblastoma.

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    Siar, Chong Huat; Ng, Kok Han

    2014-01-01

    Epithelial-to-mesenchymal transition (EMT) via the mechanism of transcription repression is a crucial process for the induction of invasiveness in many human tumors. Ameloblastoma is a benign odontogenic epithelial neoplasm with a locally infiltrative behavior. Twist, an EMT promoter, has been implicated in its invasiveness. The roles of the other transcription factors remain unclarified. Four transcription factors, namely Snail, Slug, SIP1, and Twist, were examined immunohistochemically in 64 ameloblastoma [18 unicystic (UA), 20 solid/multicystic (SA), 4 desmoplastic (DA), and 22 recurrent (RA)]. All four transcription factors were differentially expressed in ameloblastoma [Snail: n = 60/64 (94%); Slug: n = 21/64 (33%); SIP: n = 18/64 (28%); Twist: n = 26/64 (41%)] (P 0.05). Intracellular protein localization was predominantly nuclear for Snail, cytoplasmic>nuclear for Slug and SIP1, and cytoplasmic/nuclear for Twist. Overexpression of Snail in most subsets (UA = 18/18; SMA = 19/20; DA = 4/4; RA = 19/22) compared with the other transcription factors (P ameloblastoma. Overexpression of Snail in most subsets suggests that this molecule is most likely the prototype transcription factor involved in inducing EMT in the ameloblastoma. © 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.

  19. A Case Report of Conventional Solid Ameloblastoma presenting with Non-Classical Features

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    K S Ganapathy

    2005-01-01

    Full Text Available Ameloblastomas constitute approximately 1 percent of all cysts and tumors of the jaws. The occurrence in the mandible is four times higher than in the maxilla; in the mandible there is a predilection for the molar-ramus area with a little more than two-thirds occurring in this region. The tumor is relatively uncommon anteriorly. The average age of occurrences is in the third or fourth decade. They can grow quite large without metastasizing. The selection of treatment for an individual ameloblastoma depends to a large extent on its clinical type i. e. whether the tumor is a typical intraosseous solid or Multicystic ameloblastoma with poorly defined margins. Other factors of importance are its location in the jaws and its size, the age of the patient and patients availability for follow up examination.

  20. Maxillary ameloblastoma in an elderly patient: Report of a surgical approach

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    Lucas Machado de Menezes

    2017-11-01

    Full Text Available Ameloblastoma is an odontogenic benign tumor of epithelial origin that is characterized by slow, aggressive growth and invasive features. This disease is predominant in the mandible, and in adults between 30 and 40 years of age. It rarely occurs in the maxilla; there are few reported cases in literature, with relative proportions of 1:8 and 1:14 or even 1:58 diagnosed maxilla/mandible ameloblastoma cases, according to published retrospective studies. The purpose of this study is to present and discuss a case of ameloblastoma in the maxilla of an elderly man from the state of Pará in Brazil, who presented with a lesion in the left maxilla. The clinical and surgical steps were chosen based on a set of criteria that will be discussed in this report.

  1. Regulation of IL-6 and IL-8 production by reciprocal cell-to-cell interactions between tumor cells and stromal fibroblasts through IL-1α in ameloblastoma.

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    Fuchigami, Takao; Kibe, Toshiro; Koyama, Hirofumi; Kishida, Shosei; Iijima, Mikio; Nishizawa, Yoshiaki; Hijioka, Hiroshi; Fujii, Tomomi; Ueda, Masahiro; Nakamura, Norifumi; Kiyono, Tohru; Kishida, Michiko

    2014-09-05

    Ameloblastoma is an odontogenic benign tumor that occurs in the jawbone, which invades bone and reoccurs locally. This tumor is treated by wide surgical excision and causes various problems, including changes in facial countenance and mastication disorders. Ameloblastomas have abundant tumor stroma, including fibroblasts and immune cells. Although cell-to-cell interactions are considered to be involved in the pathogenesis of many diseases, intercellular communications in ameloblastoma have not been fully investigated. In this study, we examined interactions between tumor cells and stromal fibroblasts via soluble factors in ameloblastoma. We used a human ameloblastoma cell line (AM-3 ameloblastoma cells), human fibroblasts (HFF-2 fibroblasts), and primary-cultured fibroblasts from human ameloblastoma tissues, and analyzed the effect of ameloblastoma-associated cell-to-cell communications on gene expression, cytokine secretion, cellular motility and proliferation. AM-3 ameloblastoma cells secreted higher levels of interleukin (IL)-1α than HFF-2 fibroblasts. Treatment with conditioned medium from AM-3 ameloblastoma cells upregulated gene expression and secretion of IL-6 and IL-8 of HFF-2 fibroblasts and primary-cultured fibroblast cells from ameloblastoma tissues. The AM3-stimulated production of IL-6 and IL-8 in fibroblasts was neutralized by pretreatment of AM-3 cells with anti-IL-1α antibody and IL-1 receptor antagonist. Reciprocally, cellular motility of AM-3 ameloblastoma cells was stimulated by HFF-2 fibroblasts in IL-6 and IL-8 dependent manner. In conclusion, ameloblastoma cells and stromal fibroblasts behave interactively via these cytokines to create a microenvironment that leads to the extension of ameloblastomas. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Mandibular Reconstruction in Ameloblastoma Using Allogeneic Cord Stem Cells and Alloplastic Graft Material - Case Report.

    Science.gov (United States)

    Manimaran, K; Chandramohan, M; Kannan, R; Sankaranarayanan, S; Ravi, V R; Sharma, Rohini

    Ameloblastoma is a histologically benign odontogenic tumour and has a tendency of locally aggressive behaviour. This is second most prevalent odontogenic tumour and most common in the molar-ramus-angle region and surgical resection is only treatment option. In this article, we propose an innovative approach to deal with these cases by using alloplastic graft with cord stem cells. Over 2.5 years follow-up, we could demonstrate bone regeneration using this technique with no recurrence. To the best of our knowledge, this is the first report of successful regeneration of part of ramus and body of mandible using allogeneic cord stem cells in cases of Ameloblastoma.

  3. Conservative management of unicystic ameloblastoma in a young child: Report of two cases

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    Ritesh Kalaskar

    2011-01-01

    Full Text Available Unicystic ameloblastoma is a rare, benign, locally invasive odontogenic neoplasm of young age that show clinical, radiographic, or gross features of an odontogenic cyst, but histologically shows typical ameloblastomatous epithelium lining part of the cyst cavity, with or without luminal and/or mural tumor growth. The article presents atypical cases of a large, asymptomatic unicystic ameloblastoma of posterior maxilla and mandibular molar-ramus regions which were treated by surgical enucleation and application of Carnoy′s solution for 3 min. The article also describes the importance and complexity of differential diagnosis of an odontogenic lesion sharing common clinical and radiographical features.

  4. Oral Verrucous Carcinoma and Ameloblastoma: A Rare Coincidence

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    Zohreh Dalirsani

    2015-03-01

    Full Text Available Introduction: Oral verrucous carcinoma (OVC is a rare malignancy of the oral cavity that was first described by Ackerman. This tumor is a well-differentiated low-grade, slow growing cancer that is locally invasive without metastasis. Ameloblastoma is one of the most common odontogenic tumors, which originates from the odontogenic epithelium. Verrucous carcinoma along with central ambloblastoma is a rare phenomenon.   Case Report: A case of verrucous carcinoma along with central ambloblastoma in a 49-year-old man, which was referred with a painless exophytic lesion with a verrucous and granular surface, is reported.  Panoramic radiography revealed a well-defined radiolucency with sclerotic borders. To the best available knowledge, this phenomenon has not yet been reported.   Conclusion:  Verrucous carcinoma could occur in the wall of odontogenic cysts and tumors and should be considered during the differential diagnosis of a radiolucency, which is observed in the jaws with rapid growth or which presents some changes from its previous appearance. 

  5. Ameloblastoma in a wild black rat snake (Pantherophis alleghaniensis).

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    Comolli, Jessica R; Olsen, Haley M H; Seguel, Mauricio; Schnellbacher, Rodney W; Fox, Andrew J; Divers, Stephen J; Sakamoto, Kaori

    2015-07-01

    Reports of neoplasia in captive reptiles are becoming more frequent; however, there is still scarce knowledge of the occurrence of neoplasia in wild reptiles. A wild black rat snake (Pantherophis alleghaniensis) was presented to the Zoological Medicine service of the University of Georgia's Veterinary Teaching Hospital with a 3 cm in diameter solid mandibular mass that was partially ulcerated. Radiographically, the mass was radiopaque with small bone spicules and partial osteolysis of the adjacent mandible. Histologic examination of the mass revealed a neoplasm composed of cuboidal to polygonal cells arranged in islands, anastomosing cords, and trabeculae of pseudostratified epithelium with a palisading peripheral layer of densely packed columnar cells with cytoplasmic clearing. The neoplastic tissue was separated from the mesenchyme by a prominent band of fine collagen. Neoplastic cells were positive for cytokeratin and negative for smooth muscle actin. Electron microscopy highlighted the presence of tonofilaments and microvilli. These findings led to the diagnosis of ameloblastoma, an odontogenic epithelial tumor known to occur in humans and most veterinary species. © 2015 The Author(s).

  6. Ameloblastoma vs basal cell carcinoma: an immunohistochemical comparison.

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    Jawad, Salam N; Abdullah, Bashar H

    2016-12-01

    Despite behavioral mimicry of ameloblastoma (AB) and basal cell carcinoma (BCC), they are classified at 2 extremes within pertinent WHO classifications with respect to benign and malignant designation. This study aims to appraise the current allocation of AB in the classification through an immunohistochemical comparison of some aspects of behavior with BCC. Sections from retrospectively retrieved formalin-fixed, paraffin-embedded tissue blocks of AB (n = 37) and BCC (n = 34) were comparatively examined for the immunohistochemical expression for Ki-67, Bcl-2, MMP-2, MMP-9, CD31, and D2-40 monoclonal antibodies. No statistically significant differences between the tumors were found regarding the immunoexpressions of Bcl-2 (P = .252), CD31 microvessel density (P = .895), lymphatic vessel density (P = .642), and MMP-9 stromal expression (P = .083). MMP-2 expression was significantly higher in epithelial and stromal regions of AB (P = .009 and P = .001, respectively), whereas Ki-67 and MMP-9 epithelial expressions were significantly higher in BCC (P < .000 and P = .026, respectively). Within the studied immunohistochemical attributes for tumor behavior, the study accentuated the overall behavioral mimicry of the tumors and indicated that BCCs surmount ABs by the proliferative rate only. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. Mandibular reconstruction with tissue engineering in multiple recurrent ameloblastoma.

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    Hernández-Alfaro, Federico; Ruiz-Magaz, Vanessa; Chatakun, Punjamun; Guijarro-Martínez, Raquel

    2012-06-01

    The aim of this paper is to present a new approach to bone regeneration in a patient with multiple recurrent ameloblastoma of the left mandibular angle. Through an extraoral approach, complete resection of the tumor was achieved. Bone marrow aspirate from the iliac crest was centrifuged to concentrate the mesenchymal cellular fraction. Based on a stereolithographic cast, titanium mesh was bent preoperatively to accurately reconstruct the mandibular angle. The mesh was filled with two blocks of xenogenic material mixed with recombinant bone morphogenetic protein 7 (BMP-7) and stem cells. Nine months later, three endosseous implants were placed in the regenerated bone to restore the patient's masticatory function. At this time, bone samples were obtained for histomorphometric analysis. New bone formation was confirmed around the particles of xenograft material. The results indicate that adequate esthetics and function may be achieved with bone marrow aspirate seeded on a scaffold obtained from bovine xenograft blocks and BMP-7. This technique attains new bone formation with sufficient quantity and quality to allow for implant placement, with decreased patient morbidity and surgical time compared to conventional reconstructive methods.

  8. Cell cycle aberration in ameloblastoma and adenomatoid odontogenic tumor: As evidenced by the expression of p53 and survivin.

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    Shaikh, Zulfin; Niranjan, K C

    2015-01-01

    p53 and survivin are involved in cell cycle progression and inhibition of apoptosis, respectively. Survivin is a unique protein which functions in progression of cell division and inhibits apoptosis leading to cell proliferation and cell survival. According to the literature, mutation of p53 leads to promotion of survivin function. Thus, the importance of cell cycle aberration and uncontrolled proliferation resulting from mutation of p53 and up-regulation of survivin is discussed. To assess the role of p53 and survivin in ameloblastoma and adenomatoid odontogenic tumor (AOT). The percentages of positive tumor cells were considered for statistical evaluation. Nuclear labeling index for p53 and nuclear, cytoplasmic and combined labeling index for survivin was obtained from the stained slides. Immunohistochemical expression of p53 and survivin was done qualitatively and quantitatively in 25 cases each of ameloblastoma and AOT. Mann-Whitney U-test, Wilcoxon signed ranks test and Pearson's correlation test. Quantitatively, p53 and survivin expression was statistically significant in AOT (P = 0.003) and qualitatively, in ameloblastoma (P = 0.004). Survivin expression was significant (P = 0.002) between the study groups unlike that of p53 (P = 0.554). There was no much difference in p53 expression in ameloblastoma and AOT suggestive of cell cycle aberration in both the odontogenic tumors, but significant difference in survivin expression in ameloblastoma and AOT with higher percentage of positive cells in ameloblastoma may be indicative of an aggressive behavior of ameloblastoma.

  9. Establishing the natural history and growth rate of ameloblastoma with implications for management: systematic review and meta-analysis.

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    Chae, Michael P; Smoll, Nicolas R; Hunter-Smith, David J; Rozen, Warren Matthew

    2015-01-01

    Ameloblastoma is the second most common odontogenic tumor, known to be slow-growing, persistent, and locally aggressive. Recent data suggests that ameloblastoma is best treated with wide resection and adequate margins. Following primary excision, bony reconstruction is often necessary for a functional and aesthetically satisfactory outcome, making early diagnosis paramount. Despite earlier diagnosis potentially limiting the extent of resection and reconstruction, an understanding of the growth rate and natural history of ameloblastoma has been notably lacking from the literature. A systematic review of the literature was conducted by reviewing relevant articles from PubMed and Web of Science databases. Each article's level of evidence was formally appraised according to the Centre of Evidence Based Medicine (CEBM), with data from each utilized in a meta-analysis of growth rates for ameloblastoma. Literature regarding the natural history of ameloblastoma is limited since the tumor is immediately acted upon at its initial detection, unless the patient voluntarily refuses a surgical intervention. From the limited data, it is derived that the highest estimated growth rate is associated with solid, multicystic type and the lowest rate with peripheral ameloblastomas. After meta-analysis, the calculated mean specific grow rate is 87.84% per year. The growth rate of ameloblastoma has been demonstrated, offering prognostic and management information, particularly in cases where a delay in management is envisaged.

  10. PTCH-1 and MDM2 expression in ameloblastoma from a West African sub-population: implication for chemotherapeutics.

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    Udeabor, Samuel Ebele; Adisa, Akinyele Olumuyiwa; Lawal, Ahmed Oluwatoyin; Barbeck, Mike; Booms, Patrick; Sader, Robert Alexander; Ghanaati, Shahram

    2015-01-01

    Ameloblastoma is a slow growing, painless odontogenic swelling which can attain sizes that result in severe deformities of the craniofacial complex. It is the most commonly encountered odontogenic tumor in Nigeria. Surgical intervention is currently the method of treatment; however identification of altered molecular pathways may inform chemotherapeutic potential. The Protein Patched homolog 1 (PTCH-1) is overexpressed in ameloblastoma. Also, mutation in the MDM2 gene can reduce the tumor suppressor function of p53 and promote ameloblastoma growth. No study however has characterized the molecular profile of African cases of ameloblastoma with a view to developing chemotherapeutic alternatives. The objective was to characterize the PTCH-1 genetic profile of Ameloblastoma in Nigerian patients as a first step in investigating its potential for chemotherapeutic intervention. Twenty-eight FFPE blocks of ameloblastoma cases from Nigerian patients were prepared for antibody processing to PTCH-1 (Polyclonal Anti-PTCH antibody ab39266) and MDM2 (Monoclonal Anti-MDM2 antibody (2A10) ab16895). Cytoplasmic brown staining was considered as positive for PTCH while nuclear staining was positive for MDM2. Moderate and strong expressions for PTCH in ameloblast and stellate reticulum were 78.6% and 60.7% respectively. Only 3 (10.7%) cases expressed MDM2. The importance of our study is that it supports, in theory, anti-PTCH/SHH chemotherapeutics for Nigerian ameloblastoma cases and also infers the possible additional use of anti-p53 agents.

  11. Soft tissue recurrent ameloblastomas also show some malignant features: A clinicopathological study of a 15-year database

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    Lin, Zitong; Sun, Guowen; Hu, Qingang; Chen, Fei; Wen, Shanhui

    2015-01-01

    Background To investigate the clinicopathological features of six cases of soft tissue recurrent ameloblastoma and explore the role of increased aggressive biological behavior in the recurrences and treatment of this type of ameloblastomas. Material and Methods In this study, we retrospectively reviewed recurrent ameloblastomas during a 15-year period; six cases were diagnosed as soft tissue recurrent ameloblastoma. The clinical, radiographic, cytological and immunohistochemical records of these six cases were investigated and analyzed. Results All the six soft tissue recurrent ameloblastomas occurred after radical bone resection, and were located in the adjacent soft tissues around the osteotomy regions. In Case 4, the patient developed pulmonary metastasis, extensive skull-base infiltration and cytological malignancy after multiple recurrences and malignant transformation was diagnosed. In the other five cases, although there were no cytological signs are sufficient to justify an ameloblastoma as malignant, some malignant features were observed. In Case 1, the tumor showed moderate atypical hyperplasia and the Ki-67 staining percentage was 40% positive, which are strongly suggestive of potential malignance. In Case 5, the patient developed a second soft tissue recurrence in the parapharyngeal region and later died of tumor-related complications. All the remaining three patients showed cytology atypia of varying degrees and high expression of PCNA or Ki-67, which confirmed active cell proliferation. Conclusions Increased aggressiveness is an important factor of soft tissue recurrence. An intraoperative rapid pathological examination and more radical treatment are suggested for these cases. Key words: Ameloblastoma, soft tissue recurrence, aggressive biological behaviour. PMID:25662548

  12. COX-2 as a determinant of lower disease-free survival for patients affected by ameloblastoma.

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    Montezuma, Marco Aurélio Petroni; Fonseca, Felipe Paiva; Benites, Bernar Monteiro; Soares, Ciro Dantas; do Amaral-Silva, Gleyson Kleber; de Almeida, Oslei Paes; Soares, Fernando Augusto; Pagano, Rosana Lima; Fregnani, Eduardo Rodrigues

    2018-03-15

    Ameloblastoma is a locally aggressive neoplasm with a poorly understood pathogenesis. Therefore, the aim of this study is to investigate whether COX-2 expression is associated with ameloblastoma microvascular density (MVD) and with tumor aggressiveness. Sixty-three cases of primary ameloblastomas arranged in tissue microarray were submitted to immunohistochemistry against cyclooxigenase-2 (COX-2) and CD34. Clinicopathological parameters regarding sex, age, tumour size, tumour duration, tumour location, treatment, recurrences, radiographic features, vestibular/lingual and basal cortical disruption and follow-up data were obtained from patients' medical records and correlated with the proteins expression. The results on BRAF-V600E expression were obtained from our previous study and correlated with COX-2 and CD34 expressions. Log-rank univariate analysis and multivariate Cox regression model were done to investigate the prognostic potential of the molecular markers. Twenty-eight cases (44.4%) exhibited cytoplasmic positivity for COX-2, predominantly in the columnar peripheral cells, with a mean MVD of 2.2 vessels/mm 2 . COX-2 was significantly associated with recurrences (p COX-2 was significantly associated with a lower 5-year disease-free survival (DFS) rate (p COX-2 expression in ameloblastomas is not associated with MVD, but it is significantly associated with recurrences and with a lower DFS. Copyright © 2018 Elsevier GmbH. All rights reserved.

  13. Are podoplanin and ezrin involved in the invasion process of the ameloblastomas?

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    Y.F. Costa

    2015-02-01

    Full Text Available The association between podoplanin and ezrin in the process of odontogenic tumors invasion has been suggested, but was not studied yet. Our purpose was to investigate the relationship between podoplanin and ezrin expressions in the odontogenic epithelium of ameloblastomas. Forty-seven ameloblastomas were analyzed by immunohistochemistry using anti-podoplanin and anti-ezrin antibodies. The expressions of both proteins were evaluated using a score method and the comparison and association between these proteins were verified, respectively, by Wilcoxon Signed-Rank test and by Spearman’s rank correlation coefficient, using a statistical significance level of 0.05. The majority of tumors (87.2% exhibited strong membranous expression of podoplanin in the peripheral cells. Cytoplasmic expression of ezrin in the peripheral cells of ameloblastomas was stronger than its membranous expression. No statistically significant correlation was observed between podoplanin and ezrin. However, there was statistically significant difference between membranous podoplanin and membranous ezrin expressions, between cytoplasmic podoplanin and membranous ezrin expressions, and between cytoplasmic podoplanin and cytoplasmic ezrin expressions. There was no statistical difference between membranous podoplanin and cytoplasmic ezrin expressions. These results suggest a synergistic role of both proteins in the process of invasion of ameloblastomas.

  14. Radiological challenges in distinguishing keratocystic odontogenic tumor from ameloblastoma: an extraordinary occurrence in the same patient.

    Science.gov (United States)

    Moubayed, Sami P; Khorsandi, Azita; Urken, Mark L

    2016-01-01

    The aims of this study are to describe the radiological appearance of two common odontogenic lesions (keratocystic odontogenic tumor and ameloblastoma) arising in the same patient simultaneously with their radiological differences and histological correlates, and to describe challenges in radiological diagnosis. Single case report. Tertiary referral center. Forty-one year-old African-American male patient. Lesion appearance on computed tomography (CT) scan and pathological correlates. A 41year-old African-American male presented with asymptomatic right maxillary swelling. A CT scan of the maxillofacial skeleton showed and expansile and cystic lesion of the right hemimaxilla with trabecular osseous expansion, and a left cystic lesion in the left hemimaxilla expanding into the pterygopalatine fossa. Biopsy confirmed the suspected diagnoses of right ameloblastoma and left keratocystic odontogenic tumor. Although they are among the most common odontogenic tumors, the presence of concurrent ameloblastoma and KOT is an exceedingly rare occurrence in the same patient. The appearance on CT scan may help in distinguishing ameloblastoma from KOT by looking at bone expansion and high density areas, although the gold standard diagnostic test remains open biopsy. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Magnesium-enriched hydroxyapatite as bone filler in an ameloblastoma mandibular defect.

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    Grigolato, Roberto; Pizzi, Natalia; Brotto, Maria C; Corrocher, Giovanni; Desando, Giovanna; Grigolo, Brunella

    2015-01-01

    The aim of this study was to evaluate the clinical performance of a magnesium-enriched hydroxyapatite biomaterial used as bone substitute in a case of mandibular ameloblastoma treated with conservative surgery. A 63 year old male patient was treated for an ameloblastoma in the anterior mandibular profile. After tissue excision, the bone defect was filled with a synthetic hydroxyapatite biomaterial enriched with magnesium ions, in order to promote bone tissue regeneration and obtain a good aesthetic result. Twenty-five months after surgery, due to ameloblastoma recurrence in an area adjacent to the previously treated one, the patient underwent to a further surgery. In that occasion the surgeon performed a biopsy in the initially treated area, in order to investigate the nature of the newly-formed tissue and to evaluate the bone regenerative potential of this biomaterial by clinical, radiographic and histological analyses. The clinical, radiographic and histological evaluations showed various characteristics of bone remodeling stage with an ongoing osteogenic formation and a good osteo-integration. In conclusion, magnesium-enriched hydroxyapatite used as bone substitute in a mandibular defect due to ameloblastoma excision showed an effective bone regeneration at 25 months follow-up, demonstrating an excellent biocompatibility and a high osteo-integration property.

  16. Ameloblastoma of the jaws: a critical reappraisal based on a 40-years single institution experience

    NARCIS (Netherlands)

    Hertog, D.; van der Waal, I.

    2010-01-01

    The 40-years of experience in a single institution with the treatment of previously untreated ameloblastoma have been reported, followed by a management protocol. Retrospectively, 25 consecutive patients treated between 1969 and 2009 have been analyzed. In 11 patients, a preoperative diagnosis of

  17. Pattern and prevalence of ameloblastoma in a tertiary hospital – a ...

    African Journals Online (AJOL)

    Background: Ameloblastoma is the commonest odontogenic tumour afflicting our populace. Furthermore, its management has been engulfed in controversy.As a tertiary institution serving as a referral center to neighboring states, no evaluation of demographics, clinical, radiological and pathological picture of ...

  18. An unusual case of ameloblastoma observed in the left anterior mandible.

    Science.gov (United States)

    Canger, Emin Murat; Çelenk, Peruze; Bulut, Emel; Günhan, Ömer

    2014-11-01

    To report a small intaalveolar ameloblastoma which resembled cystic lesion, and to emphasize the value of Computed tomography (CT) in radiographic examination. Ameloblastoma is a slow-growing, locally invasive odontogenic neoplasm that accounts for approximately 10% of all tumors detected in the jaws. Radiographically, those tumors are usually well-defined. Computerized tomography is highly recommended to confirm the diagnosis. A 48-year-old female attended with a chief complaint of painless swelling in the left anterior of mandible, 1 month duration. In intraoral examination, non-fluctuant, immobile, approximately 1 × 1 cm in dimension, painless, swelling which had a bone-like hardness and located in the mandibular canine region was detected. Panoramic radiography revealed a well circumscribed unilocular radiolucent lesion located in the inter-radicular area of left mandibular lateral and canine teeth. In CT examination it was realized that the lesion was multilocular. Histopathological examination of the biopsy specimen was reported as ameloblastoma. It is extremely difficult to find such an ameloblastoma in small dimension in alvolar bone.

  19. Surface vacuolar ATPase in ameloblastoma contributes to tumor invasion of the jaw bone.

    Science.gov (United States)

    Yoshimoto, Shohei; Morita, Hiromitsu; Matsubara, Ryota; Mitsuyasu, Takeshi; Imai, Yuko; Kajioka, Shunichi; Yoneda, Masahiro; Ito, Yushi; Hirofuji, Takao; Nakamura, Seiji; Hirata, Masato

    2016-03-01

    Ameloblastoma is the most common benign odontogenic tumor in Japan. It is believed that it expands in the jaw bone through peritumoral activation of osteoclasts by receptor activator of nuclear factor kappa-B ligand (RANKL) released from the ameloblastoma, as in bone metastases of cancer cells. However, the clinical features of ameloblastoma, including its growth rate and patterns of invasion, are quite different from those of bone metastasis of cancer cells, suggesting that different underlying mechanisms are involved. Therefore, in the present study, we examined the possible mechanisms underlying the invasive expansion of ameloblastoma in the jaw bone. Expression levels of RANKL assessed by western blotting were markedly lower in ameloblastoma (AM-1) cells than in highly metastatic oral squamous cell carcinoma (HSC-3) cells. Experiments coculturing mouse macrophages (RAW264.7) with AM-1 demonstrated low osteoclastogenic activity, as assessed by tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cell formation, probably because of low release of RANKL, whereas cocultures of RAW264.7 with HSC-3 cells exhibited very high osteoclastogenic activity. Thus, RANKL release from AM-1 appeared to be too low to generate osteoclasts. However, AM-1 cultured directly on calcium phosphate-coated plates formed resorption pits, and this was inhibited by application of bafilomycin A1. Furthermore, vacuolar-type H+-ATPase (V-ATPase) and H+/Cl- exchange transporter 7 (CLC-7) were detected on the surface of AM-1 cells by plasma membrane biotinylation and immunofluorescence analysis. Immunohistochemical analysis of clinical samples of ameloblastoma also showed plasma membrane-localized V-ATPase and CLC-7 in the epithelium of plexiform, follicular and basal cell types. The demineralization activity of AM-1 was only 1.7% of osteoclasts demineralization activity, and the growth rate was 20% of human normal skin keratinocytes and HSC-3 cells. These results suggest that the

  20. Analisis inmunohistoquimico de CK14 y CK19 en germen dentario y ameloblastoma

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    Delmira Apellaniz

    Full Text Available Resumen Introducción. La odontogénesis es el proceso de formación de los órganos dentarios, en el cual se expresan diversas moléculas, dentro de las cuales encontramos las citoqueratinas 14 y 19 (CK14, CK19. Una vez concluido el proceso de formación del diente quedan restos del epitelio odontogénico, el cual se ha sugerido se encuentra implicado en el desarrollo del ameloblastoma, uno de los tumores odontogénicos más frecuentes. Se ha sugerido que las CK14 y CK19 tienen utilidad como marcadores de diferenciación ameloblástica, y podrían tener implicación dentro del comportamiento tumoral de los ameloblastomas. El objetivo del presente estudio fue describir los patrones de expresión inmunohistoquímica de estas dos citoqueratinas en gérmenes dentarios y ameloblastomas. Materiales y métodos. Se incluyeron 6 ameloblastomas sólidos multiquísticos y 5 gérmenes dentarios a los cuales se les realizó técnica de inmunohistoquímica para CK14 y CK19. Resultados. Este estudio permitió visualizar la inmunoexpresión de CK14 y CK19 en el epitelio y la negatividad en el ectomesénquima, tanto en los gérmenes dentarios como en ameloblastomas. También permitió concluir que CK19 puede ser considerada como un eficiente marcador de diferenciación ameloblástica, mientras que CK14 es gradualmente remplazada por CK19 en el epitelio interno del órgano del esmalte, evidenciándose marcada inmunoexpresión de esta última en ameloblastos secretores

  1. Expression of CD34 and maspin in ameloblastoma from a West African subpopulation.

    Science.gov (United States)

    Udeabor, S E; Adisa, A O; Kolude, B; Barbeck, M; Kirkpatrick, C J; Sader, R A; Ghanaati, S

    2014-08-01

    Ameloblastoma is a locally invasive odontogenic tumor with a high recurrence rate. Its local invasiveness is aided by angiogenesis, which can be correctly estimated by CD34. On the other hand, maspin decreases the local invasive and metastatic capability of cancer cells and functions as an angiogenesis inhibitor. We aim to assess the association between maspin expression and microvessel density in ameloblastoma. Twenty-five formalin-fixed paraffin-embedded (FFPE) blocks of ameloblastoma cases were prepared for antibody processing to CD34 and maspin. Positive immunohistochemical staining was marked by brown cytoplasmic/membrane coloration for CD34 and by nuclear/cytoplasmic coloration for maspin. At the ×40 magnification, we counted blood vessels in two areas of dimension; 300 × 400 μm (area A) and 150 × 200 μm (area B) adjacent to the tumor region to assess relative dispersion of the vessels bordering the tumor. The overall approximate microvessel density (MVD) for area A = 11 (minimum 2, maximum 21) and that for area B = 5 (minimum 1, maximum 10). The MVD in the area A of plexiform ameloblastoma was similar to that of the unicystic, while the hemangiomatous variant had the highest MVD for area A. Maspin positivity was present only in the cytoplasm of ameloblast, stellate reticulum, and the fibrous connective tissue in varying proportions. There was no evidence of the anti-angiogenesis effect of maspin in ameloblastoma from this study. The significance of cytoplasmic localization of maspin in the ameloblasts and stellate reticulum cells needs further investigation.

  2. Ameloblastoma en población del noreste de Brasil: un estudio retrospectivo de 60 casos

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    Thiago de Santana Santos

    2010-06-01

    Full Text Available El ameloblastoma es un tumor de epitelio odontogénico, caracterizado por la invasión local y aumento de la tasa de recidiva. Por esto se realizó este estudio descriptivo para determinar el comportamiento de los ameloblastomas en nuestro medio. Método: estudio retrospectivo de 17 años basado en los datos existentes de los archivos del Departamento Oral de Patología. Se analizaron los archivos de pacientes de enero de 1992 a mayo de 2009, con un diagnóstico definitivo de ameloblastoma. Fué recogida la información respecto al sexo, raza, presencia de síntomas, ubicación anatómica y extensión. Resultados: dentro de los 60 pacientes con ameloblastoma se observó, mayor incidencia en la tercera década de vida (28,3 %. Las mujeres fueron más comprometidas que los hombres, con una proporción 1,22:1. La mayoría de lesiones fueron observadas en la raza blanca (51,7 %. El sitio más habitual por la ocurrencia de tumor fue la mandíbula (85 %. Los pacientes fueron sintomáticos en el 75 %. La mayoría de pacientes presentaron lesiones de 3,5 a 6,0 centímetros de extensión (55 %. Conclusiones: el ameloblastoma es mayormente observado en mujeres, en la tercera década de la vida y en la mandíbula. Las diferencias con otros estudios entre el sexo, la edad, raza, extensión, localización anatómica y los síntomas pueden ser atribuidos a las diferencias geográficas.

  3. The approach and treatment of a unicystic Ameloblastoma in the jaw - doi:10.5020/18061230.2006.p247

    Directory of Open Access Journals (Sweden)

    Antônio Francisco Durighetto Júnior

    2012-01-01

    Full Text Available Among the complex group of odontogenic tumors, the ameloblastoma points out as the most important and common odontogenic tumor of epithelial origin. It consists mainly of a benign tumor, slow in progress and locally invasive, although it can present high rates of reincidence. The unicystic ameloblastoma is one of the tumor variants and deserves singular consideration for its peculiar characteristics. This study aimed at showing the peculiarities of this ameloblastoma variant through a case report on the care assistance of a young man committed by a jaw tumor, approaching the diagnostic methods used and the applied treatment. Based on present, we conclud that the prognostic for patients with unicystic ameloblastoma is very favorable, since the propaedeutic proceedings are well conducted by the professionals involved.

  4. Immunohistochemical expression of podoplanin (D2-40), lymphangiogenesis, and neoangiogenesis in tooth germ, ameloblastomas, and ameloblastic carcinomas.

    Science.gov (United States)

    Sánchez-Romero, Celeste; Bologna-Molina, Ronell; Mosqueda-Taylor, Adalberto; de Almeida, Oslei Paes

    2017-09-01

    Ameloblastoma is a benign but locally aggressive odontogenic tumor, while ameloblastic carcinoma is its malignant counterpart. Angiogenesis and lymphangiogenesis in malignancies have been correlated with higher aggressiveness and poor prognosis, as well as greater expression of podoplanin by tumoral cells. Immunohistochemical expression of podoplanin, CD34, and CD105 (endoglin) was evaluated in 53 ameloblastomas and three ameloblastic carcinomas; additionally, immunohistochemistry for podoplanin was also performed in 10 tooth germs. Microvessel density of blood and lymphatic vessels was calculated and compared between ameloblastomas and ameloblastic carcinomas. Immunoexpression of podoplanin by ameloblastic cells was evaluated in tooth germs, ameloblastomas, and ameloblastic carcinomas. Podoplanin was similarly expressed by odontogenic epithelial cells of tooth germs and ameloblastomas, while its expression was lower in ameloblastic carcinomas. There was no difference in microvessel density assessed by CD34 between ameloblastomas and ameloblastic carcinomas; nevertheless, the latter presented higher amounts of lymphatic and new formed blood vessels. Results suggest that podoplanin does not seem to be involved in invasion mechanisms of ameloblastic carcinomas, as its expression was decreased in the malignant tumoral cells. On the other hand, the increased lymphatic microvessel density and neoangiogenesis found in ameloblastic carcinomas could be related to its aggressiveness and potential for metastasis. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  5. Differential expression of the epithelial mesenchymal transition factors Snail, Slug, Twist, TGF-β, and E-cadherin in ameloblastoma.

    Science.gov (United States)

    Kurioka, Kagami; Wato, Masahiro; Iseki, Tomio; Tanaka, Akio; Morita, Shosuke

    2017-06-01

    Epithelial mesenchymal transition (EMT), the transition of epithelial cells into motile mesenchymal cells, plays an important role in embryogenesis, cancer invasion, and metastasis. Ameloblastomas are common epithelial odontogenic tumors, occurring exclusively in the mandible with locally invasive growth. Thirty-seven ameloblastoma cases were evaluated for the involvement of EMT by immunohistochemical staining and western blotting using antibodies against Slug, Snail, Twist, TGF-β, and E-cadherin. Double immunostaining was also performed. Slug and TGF-β were expressed in the nuclei of peripheral and stellate reticulum cells of ameloblastoma nests. Twenty cases of Snail, 36 of Slug, 8 of Twist, and 19 of TGF-β showed strong expression in tumor cells in follicular and plexiform patterns. Expression of Slug and TGF-β increased in regions where the expression of E-cadherin was reduced. EMT was found to be associated with the local invasive growth of ameloblastoma. These data suggest that reduced expression of E-cadherin and over-expression of Slug, Snail, and TGF-β induce EMT. Given that ameloblastomas are characterized by local invasiveness, EMT might be related to their development. Thus, strong expression of Slug and TGF-β and reduced expression of E-cadherin might be related to the local invasiveness of ameloblastoma.

  6. Ameloblastoma in the Northeast region of Brazil: A review of 112 cases

    Science.gov (United States)

    Santos, Thiago de Santana; Piva, Marta Rabello; Andrade, Emanuel Sávio de Souza; Vajgel, André; Vasconcelos, Ricardo José de Holanda; Martins-Filho, Paulo Ricardo Saquete

    2014-01-01

    Context: Ameloblastomas are benign tumors of the jaws with locally invasive capacity. Aim: The aim of this study was to review 112 cases of ameloblastoma seen over an 18-year period (1992-2009) at the Pernambuco Dental School, University of Pernambuco and at Federal University of Sergipe, in the northeast region of Brazil. Materials and Methods: The following data were selected for analysis: age, gender, race, site distribution, radiographic appearance, association with an impacted tooth, size, presence of symptoms, clinicopathologic subtypes and recurrence. Settings and Design: In this retrospective study, Pearson's χ2 test and t-test were employed. The critical level of significance was set at P < 0.05. Results: The mean age of the patients at presentation was 35.1 ± 16.8 years with a slight female preference. The peak prevalence was in the 11- to 20-year age group and declined with increasing age. Total 75 patients were black and 37 were white, for a 2:1 black: white ratio. The location of the ameloblastomas showed a marked predominance in the mandible (84.8%) and 69% of the cases presented with a multilocular radiographic appearance. The tumor was associated with an embedded tooth in 14 cases (12.7%): nine unilocular and five multilocular ameloblastomas. The maximum radiological extension of the lesions on panoramic radiographs was 0.5-20 cm (mean ± SD: 5.2 ± 3.3 cm) and most cases were symptom-free (75.9%). Solid/multicystic ameloblastoma was the most common clinicopathologic subtype. There was an association between the clinicopathologic subtypes and radiographic appearance (P < 0.001). Recurrence was observed in 13.3% of cases. Conclusion: We propose that racial factors may have strong influence on the incidence of ameloblastomas in the northeast region of Brazil, since most people have African descent. Data related to gender, location, radiographic appearance, size, symptoms, clinicopathologic subtypes and recurrence were similar to previous studies

  7. Computed tomographic and magnetic resonance imaging of ameloblastoma: 2 case reports

    Energy Technology Data Exchange (ETDEWEB)

    Oder, P.; Royster, A. [Boston Medical Center, Dept. of Radiology, Boston, Massachusetts (United States); Gibbons, D. [Boston Medical Center, Dept. of Pathology, Boston, Massachusetts (United States); Mulligan, N.; Kavanagh, P.; Eustace, S. [Boston Medical Center, Dept. of Radiology, Boston, Massachusetts (United States)

    1999-12-01

    Cysts of the mandible are uncommon. Most arise from epithelium lining the alveolus or root of the tooth (tooth derivatives), and the rest arise from the cortical and cancellous osseous matrix of the mandible. Of cysts arising from the alveolus (odontogenic epithelium), radicular cysts are the most common, accounting for almost 90% of cases. They are almost always found either in association with a dental cavity or at the base of a devitalized, amalgam-filled tooth. Of the remaining 10% of cases, most are dentigerous cysts, arising from the outer epithelial lining of the developing tooth, which is displaced to the base of the cyst as the lesion grows. Ameloblastoma, which also arises from odontogenic epithelium, accounts for less than 1% of cases. In this case report, we review the imaging appearance, histology and management of this uncommon tumour. In doing so, we highlight signal characteristics on magnetic resonance imaging (MRI) that may allow noninvasive characterization of ameloblastoma before surgical resection. (author)

  8. Computed tomographic and magnetic resonance imaging of ameloblastoma: 2 case reports

    International Nuclear Information System (INIS)

    Oder, P.; Royster, A.; Gibbons, D.; Mulligan, N.; Kavanagh, P.; Eustace, S.

    1999-01-01

    Cysts of the mandible are uncommon. Most arise from epithelium lining the alveolus or root of the tooth (tooth derivatives), and the rest arise from the cortical and cancellous osseous matrix of the mandible. Of cysts arising from the alveolus (odontogenic epithelium), radicular cysts are the most common, accounting for almost 90% of cases. They are almost always found either in association with a dental cavity or at the base of a devitalized, amalgam-filled tooth. Of the remaining 10% of cases, most are dentigerous cysts, arising from the outer epithelial lining of the developing tooth, which is displaced to the base of the cyst as the lesion grows. Ameloblastoma, which also arises from odontogenic epithelium, accounts for less than 1% of cases. In this case report, we review the imaging appearance, histology and management of this uncommon tumour. In doing so, we highlight signal characteristics on magnetic resonance imaging (MRI) that may allow noninvasive characterization of ameloblastoma before surgical resection. (author)

  9. Histopathology of ameloblastoma of the jaws; some critical observations based on a 40 years single institution experience

    Science.gov (United States)

    Hertog, Doenja; Bloemena, Elisabeth; H A Aartman, Irene

    2012-01-01

    The aim of the present study is to examine all cases of intraosseous benign ameloblastomas treated between 1970 and 2010 in a single institution and to look for a possible correlation between the histopathological aspects and the demographical and clinical parameters, as well as the treatment outcome. The data of a total number of 44 patients were retrieved from the records. Nine patients were excluded because of doubt about the correct diagnosis (8 patients) or because of an extra-osseous presentation (1 patient). No statistically significant differences were found between the histopathological (sub)types of ameloblastomas and the demographical and clinical parameters, nor between the histopathological (sub)types and treatment outcome. Of the 28 patients treated by enucleation, in 17 patients one or more recurrences occurred, with no significant predilection for any histopathological (sub)type, including the unicystic type. There were no significant differences in the recurrence rate after enucleation in patients below and above the age of 20 years either. In six out of 17 patients with a recurrence, the recurrent lesion showed a different histopathological subtype than was encountered in the primary. In two cases a change from solid/multicystic to desmoplastic ameloblastomas was noticed. In conclusion, the current histopathological classification of benign intraosseous ameloblastoma does not seem to have clinical relevance with the possible exception of the luminal unicystic ameloblastoma that has been removed in toto, unfragmented. Since no primary desmoplastic ameloblastomas were encountered in the present study no further comments can be made on this apparently rare entity. Key words: Odontogenic tumours, ameloblastoma, histopathology. PMID:22157674

  10. EGFR signaling downstream of EGF regulates migration, invasion, and MMP secretion of immortalized cells derived from human ameloblastoma.

    Science.gov (United States)

    da Rosa, Marina Rolo Pinheiro; Falcão, Aline Semblano Carreira; Fuzii, Hellen Thais; da Silva Kataoka, Maria Sueli; Ribeiro, André L R; Boccardo, Enrique; de Siqueira, Adriane Sousa; Jaeger, Ruy G; de Jesus Viana Pinheiro, João; de Melo Alves Júnior, Sérgio

    2014-11-01

    Ameloblastoma is an odontogenic tumor characterized by local invasiveness and frequent recurrence. The surrounding stroma, composed of different cell types and extracellular matrix (ECM), may influence ameloblastoma invasive behavior. Furthermore, tumor and stromal cells secrete matrix metalloproteases (MMPs), which, in turn, can modulate the matrix and promote the release of ECM-bound growth factors. Among these growth factors, epidermal growth factor (EGF) and its receptor, EGFR, have already been shown to stimulate MMP synthesis, suggesting that an interdependent mechanism, involving MMP activity and growth factors release, may contribute to tumor invasiveness. The aim of this study was to evaluate the effects of the EGF/EGFR signaling pathway on migration, invasion, and MMP activity, in a primary cell line derived from human ameloblastoma. We established and characterized a primary cell line (AME-1) from a human ameloblastoma sample. This cell line was transduced with human papillomavirus type 16 (HPV16) E6/E7 oncogenes, generating the AME-HPV continuous cell line. EGF, MMP2, and MMP9 expression in ameloblastoma biopsies and in the AME-HPV cell line was analyzed by immunohistochemistry and immunofluorescence, respectively. Migratory activity of EGF-treated AME-HPV cells was investigated using monolayer wound assays and Transwell chambers. EGF-induced invasion was assessed in Boyden chambers coated with Matrigel. Conditioned medium from EGF-treated cells was subjected to zymography. EGFR expression in AME-HPV cells was silenced by small interfering RNA (siRNA), to verify the relationship between this receptor and MMP secretion. Ameloblastoma samples and AME-HPV cells expressed EGF, EGFR, MMP2, and MMP9. AME-HPV cells treated with EGF showed increased rates of migration and invasion, as well as enhanced MMP2 and MMP9 activity. EGFR knockdown decreased MMP2 and MMP9 levels in AME-HPV cells. EGFR signaling downstream of EGF probably regulates migration, invasion

  11. EGFR, CD10 and proliferation marker Ki67 expression in ameloblastoma: possible role in local recurrence

    Directory of Open Access Journals (Sweden)

    Abdel-Aziz Azza

    2012-02-01

    Full Text Available Abstract Background Ameloblastoma is an odontogenic neoplasm characterized by local invasiveness and tendency towards recurrence. Aims Studying the role played by EGFR, CD10 and Ki67 in the recurrence of ameloblastoma. Methods This study was carried out on 22 retrospective cases of mandibular ameloblastoma from the period from Jan 2002 to Jan 2008 with follow up period until Jan 2011 (3 to 8 years follow up peroid. Archival materials were obtained from pathology department, Mansoura university. Paraffin sections of tumor tissue from all cases were submitted for routine H&E stains and immunohistochemistry using EGFR, CD10 and Ki67 monoclonal antibodies. Statistical analysis using of clinical data for all patients, tumor type, EGFR, CD10 and Ki67 expression in relation to recurrence were evaluated. Results Among the 22 cases, 10 cases were males and 12 were females with sex ratio 1:1.2. Age ranged from 34 to 59 years old with a mean age 44.18 year. Five cases showed local recurrence within studied period and proved by biopsy. No statistically significant relation was found between local recurrence and patient age, tumor size, tumor type, EGFR expression. There was a significant relation between CD10 expression as well as Ki67 labelling index and recurrence (P value = 0.003, 0.000 respectively. Conclusion Evaluation of CD10 and Ki67 status together with conventional histological evaluation can help in providing more information about the biologic behavior of the tumor, while EGFR could be a target of an expanding class of anticancer therapies. Since ameloblastomas are EGFR-positive tumors, anti-EGFR agents could be considered to reduce the size of large tumors and to treat unresectable tumors that are in close proximity to vital structures. Virtual Slides The virtual slide(s for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1902106905645651

  12. Ameloblastoma: A clinicoradiographic and histopathologic correlation of 11 cases seen in Goa during 2008-2012

    Directory of Open Access Journals (Sweden)

    Nigel R Figueiredo

    2014-01-01

    Full Text Available Objectives: The purpose of this case series was to compare the clinical, radiographic, and histopathologic features of 11 cases of ameloblastoma that reported to the Goa Dental College and Hospital over a period of 4 years from January 2008 to December 2012. Study Design: Data with respect to the patients′ ages, sex, location of lesions, radiographic features, histopathologic features and surgical treatment and follow-up, was analyzed. Results: The mean age in males was 23.25 ± 6.99 years, while that in females was 43.43 ± 17.13 years. Seven (63.63% of the 11 subjects were females, and four (36.36% were males. 10 (90.9% of the 11 ameloblastomas were located in the mandible. Swelling was noted in all 11 patients. 10 cases showed radiographic findings, while one case was a peripheral soft-tissue variant. Seven (70% of the 10 tumors were multilocular with a well-demarcated corticated border, and three cases (30% were unilocular. Two cases showed a soap-bubble appearance. Histologically, five cases were unicystic, four were multicystic, one was desmoplastic and one was a peripheral variant. Follow-up ranged from 12 to 34 months and there were no signs of recurrence in any of the patients. Conclusions: Ameloblastomas more commonly occur in females at an older age, as compared with males. Radiographic features that could help in diagnosing ameloblastomas include its predominant occurrence in the mandible, multilocular radiolucency with well-defined, corticated, scalloped margins, expansion of buccal and lingual cortical plates, root resorption and tooth displacement.

  13. Ameloblastoma after surgical removal of an impacted mandibular molar. A case report.

    Science.gov (United States)

    Chindia, M L; Guthua, S W; Mwaniki, D L

    1991-04-01

    A case of an ameloblastoma diagnosed about 3 1/2 years after removal of an impacted mandibular 3rd molar is presented. The pre-operative radiographs, though poor in quality, showed an ill-defined radiolucency in relation to the tooth. This feature was not apparent to the examiners at the time of first presentation. Despite unfavourable working conditions in developing countries, a high level of suspicion should be maintained to avoid serious sequelae at a later stage.

  14. Extensive ameloblastoma of the jaws: surgical management and immediate reconstruction using microvascular flaps.

    Science.gov (United States)

    Zemann, Wolfgang; Feichtinger, Matthias; Kowatsch, Eberhard; Kärcher, Hans

    2007-02-01

    Ameloblastoma is a rare histological benign but locally aggressive tumor with a marked tendency for recurrence. Especially larger, aggressive lesions require a more radical surgical approach resulting in large jaw defects. The purpose of this study is to analyze the long-term functional and esthetic results after immediate reconstruction of large jaw defects using microvascular flaps. A review of 7 cases of giant ameloblastoma (2 in the maxillary and 5 in the mandibular region) is presented. The lesions were between 4 and 8 cm in diameter. All patients were treated by a radical surgical protocol. All cases were immediately reconstructed using microvascular grafts from either the scapula or the iliac crest bone. Dental implants were inserted in all patients after removal of the osteosynthesis material. All patients were prosthetically rehabilitated. All implants survived throughout the observation time. The esthetic and functional outcomes were satisfying in all patients. No case of recurrence of the tumor could be observed so far. According to our opinion, immediate reconstruction is the treatment of choice after radical surgical excision of ameloblastoma. This 1-step procedure decreases the number of surgeries and allows earlier prosthetic rehabilitation.

  15. Ameloblastoma with varied sites of metastasis: report of two cases and literature review.

    Science.gov (United States)

    Lin, Yi; He, Jian-feng; Li, Zhi-yong; Liu, Jian-hua

    2014-07-01

    We report two rare cases of lung metastasis from maxillary ameloblastoma, in order to review its risk and analyse the types of metastases that can present with this disease. A 40-year-old male with multiple recurrences and a 46-year-old female, who had undergone successful surgical treatment of a maxillary ameloblastoma, presented with metastatic lesions. The primary tumour and metastases were benign in both patients. We reviewed and analysed 20 cases of the same condition reported in recent years. Our initial treatment for the primary maxillary lesion was performed more than 10 years before the pulmonary lesions presented. Due to the aggressive nature of this tumour, metastases in the lungs and cervical lymph nodes (male patient) were confirmed. These cases presented a diagnostic challenge due to the multiple and varied sites of recurrence, which indicate the natural behaviour of this tumour. Different routes of metastasis can occur, including implanting, haematogenous, and lymphatic spread. CT-guided percutaneous transthoracic lung biopsy is an important method to confirm metastatic ameloblastoma. Copyright © 2013 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

  16. Autotransplantation of Immature Third Molars and Orthodontic Treatment After En Bloc Resection of Conventional Ameloblastoma.

    Science.gov (United States)

    Osterne, Rafael Lima Verde; Moreira Neto, José Jeová Siebra; de Araújo Lima, Augusto Darwin Moreira; Nogueira, Renato Luiz Maia

    2015-09-01

    Ameloblastoma treatment can lead to significant bone defects; consequently, oral rehabilitation can be challenging. We present the case of a 14-year-old girl diagnosed with a conventional ameloblastoma in the mandible who was treated using en bloc resection and rehabilitated with autotransplantation of the immature third molars and orthodontic treatment. The lesion was in the region of the lower left canine and premolars, and en bloc resection resulted in a significant alveolar bone defect. Autotransplantation of the lower third molars to the site of the lower left premolars was performed. After 2 years, the upper left third molar was transplanted to the site of the lower left canine. During the orthodontic treatment period, considerable alveolar bone formation was observed in the region of the transplanted teeth, and roots developed. To the best of our knowledge, this is the first reported case of alveolar bone formation induction caused by tooth transplantation after ameloblastoma treatment. Copyright © 2015 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  17. Expression of Bcl-2 and epithelial growth factor receptor proteins in keratocystic odontogenic tumor in comparison with dentigerous cyst and ameloblastoma

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    Seyed Mohammad Razavi

    2015-01-01

    Conclusion: According to the expression of - Bcl-2 in ameloblastoma and KCOT, and no expression of EGFR in KCOT, it can be concluded that the biological activity and growth mechanisms of KCOT are different compared with other cystic lesions. However, the aggressive potential of KCOT is not as severe as that of a neoplasm such as ameloblastoma.

  18. Hemimandibulektomi dengan Rekonstruksi Mandibula dan Fiksasi Intermaksila sebagai Penatalaksanaan Ameloblastoma Mandibula Sinistra

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    Indria Nehriasari

    2012-12-01

    Full Text Available Latar belakang. Ameloblastoma adalah tumor odontogenik yang jarang terjadi. Walaupun jinak tetapi bersifat merusak dan mempunyai tingkat kekambuhan yang tinggi. Hemimandibulektomy adalah salah satu tindakan yang dipilih jika lesi patologis telah melibatkan processus coronoideus dan condyle walaupun efek dari tindakan tersebut adalah terjadinya defek wajah dan deviasi mandibula. Tujuan. Melaporkan tindakan hemimandibulektomy dengan rekonstruksi bridging plate dan traksi intermaksila pada ameloblastoma mandibula sebelah kiri yang dilakukan untuk mengurangi deviasi dan defek wajah. Kasus dan perawatan. Laki-laki umur 46 tahun datang ke klinik bedah mulut dan maksilofacial RS Dr Sardjito Yogyakarta dengan keluhan utama adanya pembengkakan pada sisi kiri rahang bawah di area pipi. Keadaan tersebut dirasakan sejak 4 tahun yang lalu, tidak sakit, keras, warna sesuai dengan jaringan sekitar. Diagnosa yang ditegakkan Ameloblastoma mandibula. Perawatan dari kasus ini adalah hemimandibulektomy dengan rekonstruksi bridging plate dengan anestesi umum. Enam minggu dengan kawat dan 3 bulan dengan traksi elastic digunakan untuk mengurangi deviasi mandibula setelah tulang rahang direseksi. Kesimpulan. Hemimandibulektomi dilakukan untuk mengambil lesi patologi secara radikal untuk mencegah rekurensi. Bridging plate digunakan sebagai tindakan rekontruksi mandibula. Traksi intermaksila merupakan salah satu cara yang bisa digunakan untuk mengurangi deviasi mandibula setelah hemimandibulektomy.   Background. Ameloblastoma is an odontogenic tumor which rarely happened. Although it is benign, it can be destructive and has a high recurrency rate. Hemimandibulectomy is one kind of treatments which can be choosed if pathologic fracture has involved coronoid processus and condyle, eventhough its effect can cause mandible deviation and facial defect. Purpose. Reported a hemimandibulectomy with bridging plate reconstruction and intermaxillary function on the left mandible

  19. Analysis of Prevalence and Clinical Features of Ameloblastoma and its Histopathological Subtypes in Southeast Myanmar and Lower Northern Thailand Populations: A 13-Year Retrospective Study

    Science.gov (United States)

    2017-01-01

    Introduction Prevalence of ameloblastomas has been established worldwide but collective data of ameloblastoma in Southeast Asian countries has not been well analyzed. Aim Aim of this study was to report analysis and comparison of the prevalence and demographic data of clinical features of ameloblastoma and its histopathological variants in Southeast Myanmar and lower Northern Thailand populations. Materials and Methods A retrospective study on ameloblastoma was performed based on the availability of oral biopsy specimens in Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand, between January 2002 and August 2015. The collected data were subjected to descriptive statistical analyses with the SPSS version 17.0 statistical software package (SPSS Inc., Chicago, USA). Pearson’s chi square (χ2) test and t-test were employed. The critical level of significance was set at pameloblastoma with male:female ratio of 1.14:1. The mean age of the patients was 31.3±15.6 years. The predominance anatomical distribution was observed in the mandible (86.7%). Posterior body-ramus-angle region was the most common site. Almost all cases were asyptomatic and most common clinical manifestation was swelling of affected region. Multilocular radiolucency was observed in 70% of cases, whereas 30% were unilocular. Three subtypes of ameloblastomas were diagnosed: unicystic ameloblastoma (20%), conventional solid/multicystic ameloblastoma (70%), and desmoplastic ameloblastoma (10%). The most common histologic pattern was the plexiform type (57.2%) followed by follicular type (23.8%). Conclusion Prevalence of ameloblastoma in Southeast Myanmar and lower Northern Thailand populations correspond with data from other geographic areas of Thailand and other Asian countries. However, some demographic and histopathological profiles are different, with plexiform ameloblastoma being the most common subtype in this study. PMID:28274056

  20. An unsuspected ameloblastoma in the subpontic region of the mandible with consideration of pathogenesis from the radiographic course

    Science.gov (United States)

    2010-01-01

    The purpose of this report is to document a case of unsuspected ameloblastoma involving the right man dibular subpontic region in a 38-year-old Cambodian female patient. This lesion was purportedly preceded by multiple radiolucencies which were diagnosed as radi cular cysts and treated a few times in the past years by enucleation followed by endodontic therapy of the affected teeth. Bridgework restoration of the partially edentulous area was performed. This case report de monstrates radiographic changes that occurred in the periods before and after the diagnosis of amelo blas to ma. The case may represent an example of radicular cysts and ameloblastoma occurring as a collision phenomenon, or the ameloblastoma may have arisen as a result of neoplastic transformation of the lining epi thelium in an inflammatory odontogenic epithelial cyst. PMID:20452900

  1. Ameloblastic carcinoma developing in preexisting ameloblastoma with a mutation of the p53 gene: a case report.

    Science.gov (United States)

    Nobusawa, Aiko; Sano, Takaaki; Yokoo, Satoshi; Oyama, Tetsunari

    2014-11-01

    Ameloblastic carcinoma is a rare malignant odontogenic tumor. Here we present a case of a large ameloblastic carcinoma, which developed in a preexisting ameloblastoma in the right submandibular region. The patient was an 84-year-old woman who had received several surgical procedures for ameloblastoma, including a segmental mandibulectomy. The dimensions of the tumor were 12 × 8 × 5 cm, and both benign ameloblastoma and ameloblastic carcinoma were observed histologically. Based on histologic diagnosis, immunohistochemical staining and sequence analysis for p53 were performed. Overexpression of p53 was observed only in the ameloblastic carcinoma. Additionally, a mutation of the p53 gene (TP53) in exon 5 was found by sequence analysis in the ameloblastic carcinoma. This is the first case of ameloblastic carcinoma with a mutation of the p53 gene that has been associated with carcinomatous transformation. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Fibroblast growth factors 7 and 10 are involved in ameloblastoma proliferation via the mitogen-activated protein kinase pathway.

    Science.gov (United States)

    Nakao, Yu; Mitsuyasu, Takeshi; Kawano, Shintaro; Nakamura, Norifumi; Kanda, Shiori; Nakamura, Seiji

    2013-11-01

    Ameloblastoma is an epithelial benign tumor of the odontogenic apparatus and its growth mechanisms are not well understood. Fibroblast growth factor (FGF) 3, FGF7 and FGF10, which are expressed by the neural crest-derived ectomesenchymal cells, induce the proliferation of odontogenic epithelial cells during tooth development. Therefore, we examined the expression and function of these FGFs in ameloblastoma. We examined 32 cases of ameloblastoma as well as AM-1 cells (an ameloblastoma cell line) and studied the expression of FGF3, FGF7, FGF10 and their specific receptors, namely, FGF receptor (FGFR) 1 and FGFR2. Proliferation, mitogen-activated protein kinase (MAPK) signaling and PI3K signaling were examined in AM-1 cells after the addition of FGF7, FGF10 and these neutralizing antibodies. The expression of FGF7, FGF10, FGFR1 and FGFR2 was detected in ameloblastoma cells and AM-1 cells, while that of FGF3 was not. FGF7 and FGF10 stimulated AM-1 cell proliferation and phosphorylation of p44/42 MAPK. However, Akt was not phosphorylated. Blocking the p44/42 MAPK pathway by using a specific mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitor (U0126) completely neutralized the effects of FGF7 and FGF10 on AM-1 cell proliferation. However, Anti FGF7 and FGF10 neutralizing antibodies did not decrease cell proliferation and MAPK phosphorylation of AM-1 cells. These results suggested that FGF7 and FGF10 are involved in the proliferation of ameloblastoma cells through the MAPK pathway.

  3. Parenchyma-stromal interactions induce fibrosis by secreting CCN2 and promote osteoclastogenesis by stimulating RANKL and CD68 through activated TGF-β/BMP4 in ameloblastoma.

    Science.gov (United States)

    Takebe, Yuichiro; Tsujigiwa, Hidetsugu; Katase, Naoki; Siar, Chong Huat; Takabatake, Kiyofumi; Fujii, Masae; Tamamura, Ryo; Nakano, Keisuke; Nagatsuka, Hitoshi

    2017-01-01

    Tumor parenchyma-stromal interactions affect the properties of tumors and their dynamics. Our group previously showed that secreted frizzled related protein (sFRP)-2 impairs bone formation and promotes bone invasion in ameloblastoma. However, the effects of the secreted growth factors CCN2, TGF-β, and BMP4 on stromal tissues in ameloblastoma remain unclear. Thirty-five paraffin-embedded ameloblastoma cases, ameloblastoma-derived cell lines (AM-1), and primary cultures of ameloblastoma stromal fibroblasts (ASF) were used. Immunohistochemistry, MTT assay, Western blotting, and RT-PCR were performed on these samples. Parenchyma-stromal CCN2 overexpression correlated significantly with fibrous-type stroma, but not with myxoid-type stroma, suggesting a role of CCN2 in fibrosis (P ameloblastoma with fibrous-type stroma. These data suggest that stromal properties influence bone resorption-related activities and growth rates, respectively. These results suggest that the effects of secreted growth factors are governed by ameloblastoma parenchyma-stromal interactions. CCN2 promotes fibrogenesis independent of TGF-β signaling. Absence of CCN2 expression is associated with a phenotypic switch to a myxoid-type microenvironment that is conducive for TGF-β/BMP4 signaling to promote osteoclastogenesis. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  4. The occurrence and pattern of ameloblastoma in children and adolescents: an Indian institutional study of 41 years and review of the literature.

    Science.gov (United States)

    Bansal, S; Desai, R S; Shirsat, P; Prasad, P; Karjodkar, F; Andrade, N

    2015-06-01

    Ameloblastoma in the paediatric age group is considered a rarity and it accounts for approximately 10-15% of all reported cases. This study assessed the clinical, radiological, and histopathological features of 39 cases of ameloblastoma in Indian children aged less than 18 years, seen over a 41-year period (1971-2011) in the Department of Oral Pathology, Nair Hospital Dental College, India. Out of 256 diagnosed cases of ameloblastoma, 39 (15.2%) occurred in patients ranging in age from 4.5 to 18 years (mean age 13.6 years; male-to-female ratio 2:1). All of the tumours were intraosseous, with a marked predilection for the mandible (97.4%), the body-angle-ramus being the most commonly involved site. Radiographically, 23 cases presented as unilocular radiolucency. Histologically, 20 cases presented as solid and 19 as unicystic ameloblastoma. The interesting finding of 10 solid ameloblastoma presenting as unilocular radiolucency and five cases of unicystic ameloblastoma manifesting as multilocular radiolucency suggests that solid ameloblastomas should be included in the differential diagnosis of unilocular radiolucency of the jaw in the paediatric age group. Copyright © 2015 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  5. Clinicopathological and demographic characteristics of ameloblastomas in a population from Bahia, Brazil = Características clinicopatológicas e demográficas de ameloblastomas em uma população da Bahia, Brasil

    Directory of Open Access Journals (Sweden)

    Santos, Jean Nunes dos

    2010-01-01

    Conclusão: O perfil clínico- epidemiológico dos pacientes do presente estudo é similar ao de outras populações. Em adição, o ameloblastoma unicístico é o subtipo histológico mais comum em nossa população

  6. Histopathology of ameloblastoma of the jaws; some critical observations based on a 40 years single institution experience

    NARCIS (Netherlands)

    Hertog, D.; Bloemena, E.; Aartman, I.H.A.; van der Waal, I.

    2012-01-01

    The aim of the present study is to examine all cases of intraosseous benign ameloblastomas treated between 1970 and 2010 in a single institution and to look for a possible correlation between the histopathological aspects and the demographical and clinical parameters, as well as the treatment

  7. A prospective epidemiological study on odontogenic tumours in a black African population, with emphasis on the relative frequency of ameloblastoma.

    Science.gov (United States)

    Oginni, F O; Stoelinga, P J W; Ajike, S A; Obuekwe, O N; Olokun, B Aluko; Adebola, R A; Adeyemo, W L; Fasola, O; Adesina, O A; Akinbami, B O; Iwegbu, I O; Ogunmuyiwa, S A; Obimakinde, O S; Uguru, C C

    2015-09-01

    The persistent view in the literature is that the relative frequency of ameloblastomas is higher in the black population than in Caucasians. The aim of this study was to determine the relative frequency of all odontogenic tumours (OT) in a 100% black population and to compare our findings with those of previous studies. A prospective study was undertaken of all patients presenting with OT to all 16 Nigerian departments of oral and maxillofacial surgery over a 4-year period. The following data were obtained: patient demographics, delay to presentation, extent of the lesion, and histological diagnosis. Six hundred and twenty-two cases were studied. A slight male preponderance was observed (male to female ratio 1.17:1). Patients ranged in age from 5 to 89 years, with a peak incidence in the third decade. The relative frequency of OT was 0.99 per million and that of ameloblastoma was 0.76 per million. Ameloblastoma was the most prevalent OT (76.5%), followed by adenomatoid odontogenic tumours (5.6%), odontogenic myxoma (4.5%), and keratocystic odontogenic tumours (KCOT) (3.1%). The relative frequency of ameloblastoma among Nigerians was not different from frequencies reported previously among Caucasian and Tanzanian black populations. KCOTs were, however, rarely diagnosed in Nigerians as compared to the white population in the Western world. Copyright © 2015 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  8. Mandibular resection via mandibular-lip L flap for the treatment of extensive multicystic Ameloblastoma

    Directory of Open Access Journals (Sweden)

    Edson Martins Oliveira Junior

    2013-06-01

    Full Text Available Ameloblastoma is a benign odontogenic tumor accounting for 1% of all tumors of the maxilla and mandibula. The mandibula is committed in 85% of the cases. The tumor is locally invasive, able to infiltrate bone marrow spaces, without radiographic or macroscopic evidence. High recurrence rates are associated with different surgical techniques, which range from simple tumoral enucleation to extensive resections. The authors report a case of a 26-year old female patient with an 8-year history of progressive, non-tender, swelling of the left mandibular region. The intraoral examination showed that the floor of mouth was raised on the left side by a bulging along the bottom of the left mandibular vestibule as well as the lingual region. Over this area, there were ulcerated areas in the alveolar region of the molars and mucosal fenestration in the alveolar and lingual regions were present. A panoramic radiography revealed a multicystic lesion, resembling the soap-bubble shape endosseous lesion. The computed tomography revealed an expandable multicystic lesion compromising both mandibular cortices. The patient underwent a biopsy, which revealed the diagnosis of a multicystic variant of Ameloblastoma, with plexiform pattern, treated with left mandibular resection and immediate installation of a customized prosthesis. After a year of follow-up, no evidence of the tumor relapse was evidenced.

  9. Two Stage Enucleation and Deflation of a Large Unicystic Ameloblastoma with Mural Invasion in Mandible

    Science.gov (United States)

    Sasaki, Ryo; Watanabe, Yorikatsu; Ando, Tomohiro; Akizuki, Tanetaka

    2014-01-01

    A treatment for strategy of unicystic ameloblastoma (UA) should be decided by its pathology type including luminal or mural type. Luminal type of UA can be treated only by enucleation alone, but UA with mural invasion should be treated aggressively like conventional ameloblastomas. However, it is difficult to diagnose the subtype of UA by an initial biopsy. There is a possibility that the lesion is an ordinary cyst or keratocystic odontogenic tumor, leading to a possible overtreatment. Therefore, this study performed the enucleation of the cyst wall and deflation at first, and the pathological finding confirmed mural invasion into the cystic wall, leading to the second surgery. The second surgery enucleated scar tissue, bone curettage, and deflation, and was able to contribute to the reduction of the recurrence rate by removing tumor nest in scar tissue or new bone, enhancing new bone formation, and shrinking the mandibular expanding by fenestration. In this study, a large UA with mural invasion including condyle was treated by “two-stage enucleation and deflation” in a 20-year-old patient. PMID:25050148

  10. Acanthomatous ameloblastoma in a female Spitz dog with rare atypical histomorphology: A case study

    Directory of Open Access Journals (Sweden)

    Shubhagata Das

    2013-08-01

    Full Text Available A 6 year old female Spitz dog was admitted to Teaching Veterinary Hospital at Chittagong Veterinary and Animal Sciences University with the history of inappetance and asymmetric lower jaw. Oral inspection revealed a pair of pale enlarged lobulated mass around the third molar tooth of the left mandible. The dog was clinically diagnosed with epulis and successfully cured by surgical excision. Histopathological section of the excised masses revealed characteristic features of acanthomatous ameloblastoma with some atypical lesions. Multifocal areas of ameloblastic islands were found in the dense sheet of proliferating epithelial layer protruding towards the sub epithelial connective tissue stroma. These islands were characterized with irregular epithelial stratification at the basal layer. Besides, presence of ghost cells was the unusual findings for such case. Prominent intercellular bridging and nuclear polymorphism in odontogenic cells were other decisive characters of the lesion. Based on the histomorphological appearance, the gingival tumor was designated as canine acanthomatous ameloblastoma (CAA with atypical histomorphology. [Vet World 2013; 6(4.000: 219-222

  11. Marsupialization of unicystic ameloblastoma: A conservative approach for aggressive odontogenic tumors

    Directory of Open Access Journals (Sweden)

    Dogan Dolanmaz

    2011-01-01

    Full Text Available Unicystic ameloblastoma (UA is known as a distinct entity which has a less aggressive behavior when compared with conventional ameloblastoma. In this report, we have presented two cases of UAs, (of which one case showed a more aggressive behavior with mural invasion into the adjacent tissues and granular cell differentiation, both of which were successfully managed with enucleation following marsupialization. We aim to highlight how this method can be used for the successful management of such cases, rather than following more aggressive approaches. In both the cases, marsupialization was done for the UA lesions initially and follow-ups were maintained. When the tumor size had regressed on radiographic follow up, an enucleation procedure with ostectomy of the margins was carried out. Special importance was also given to the endodontic treatment of the teeth involved in the area of the lesion. The patients were free of the condition and did not show any signs of recurrence on radiographic follow-ups even after 30 months of the final procedure. Granular variant of UA is quite rare and had been considered to be more aggressive. Marsupialization of UA is an alternative treatment option of resection even for more aggressive variants, as long as the histological behavior of the lesion was carefully evaluated and strict radiographic follow-up is maintained.

  12. Recurrence rate following treatment for primary multicystic ameloblastoma: systematic review and meta-analysis.

    Science.gov (United States)

    Almeida, R de A C; Andrade, E S de S; Barbalho, J C; Vajgel, A; Vasconcelos, B C do E

    2016-03-01

    Opinions regarding the treatment of multicystic ameloblastoma are divergent due to its benign nature and the high rate of recurrence if not adequately excised. The aim of the present study was to perform a systematic review of the literature for a qualitative and quantitative assessment of studies addressing primary multicystic ameloblastoma with regard to treatment and recurrence. Searches were conducted of the Ovid Medline and Embase databases for articles published up to January 2014. Based on predefined eligibility criteria, studies were selected in a two-stage screening process conducted by two independent reviewers. Quality assessment of the selected articles was performed using the modified criteria of the Agency for Healthcare Research and Quality. The meta-analysis was performed using Review Manager (RevMan) software. Statistical heterogeneity was investigated by performing a χ(2) test at the 5% significance level (Pameloblastoma in comparison to radical treatment (Pameloblastoma with bone resection. Copyright © 2015 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  13. Maxillary unicystic ameloblastoma in a 6-week-old filly evaluated with computed tomography.

    Science.gov (United States)

    Smith, H L; Rosenblatt, A J; Suen, W W; Owen, H; Ahern, B J

    2017-08-01

    A 6-week-old Thoroughbred filly was presented for evaluation of an expansile mass overlying the right nasal passage and causing respiratory stertor. On skull radiographs, there was a loculated, soft tissue-opaque mass identified dorsal to the right upper premolars and effacing the right nasal cavity. Computed tomography (CT) revealed a locally extensive mass with relatively benign characteristics located centrally on the tooth root apices of the deciduous second premolar (506). The mass extended axially into the right nasal cavity, occluding the meatuses and causing displacement of the nasal septum to the left. Surgical excision was not deemed feasible with an athletic future in mind and the owners elected to euthanase the filly. Histopathologically, the mass was consistent with unicystic ameloblastoma and was lined intermittently with palisading, columnar basal cells (ameloblast-like cells) overlying a zone containing stellate cells in loose stroma. To the authors' knowledge this is the first report of a CT scan of an equine ameloblastoma. Although histopathology was essential for definitive diagnosis, CT clearly defined the origin of the mass and identified its locally extensive, cystic nature, which enabled informed decisions to be made. © 2017 Australian Veterinary Association.

  14. Two stage enucleation and deflation of a large unicystic ameloblastoma with mural invasion in mandible.

    Science.gov (United States)

    Sasaki, Ryo; Watanabe, Yorikatsu; Ando, Tomohiro; Akizuki, Tanetaka

    2014-06-01

    A treatment for strategy of unicystic ameloblastoma (UA) should be decided by its pathology type including luminal or mural type. Luminal type of UA can be treated only by enucleation alone, but UA with mural invasion should be treated aggressively like conventional ameloblastomas. However, it is difficult to diagnose the subtype of UA by an initial biopsy. There is a possibility that the lesion is an ordinary cyst or keratocystic odontogenic tumor, leading to a possible overtreatment. Therefore, this study performed the enucleation of the cyst wall and deflation at first, and the pathological finding confirmed mural invasion into the cystic wall, leading to the second surgery. The second surgery enucleated scar tissue, bone curettage, and deflation, and was able to contribute to the reduction of the recurrence rate by removing tumor nest in scar tissue or new bone, enhancing new bone formation, and shrinking the mandibular expanding by fenestration. In this study, a large UA with mural invasion including condyle was treated by "two-stage enucleation and deflation" in a 20-year-old patient.

  15. Clinicopathological evaluation of 164 dental follicles and dentigerous cysts with emphasis on the presence of odontogenic epithelium in the connective tissue. The hypothesis of "focal ameloblastoma"

    NARCIS (Netherlands)

    Meleti, M.; van der Waal, I.

    2013-01-01

    Objectives: Some ameloblastomas presumably originate from odontogenic epithelium within the connective tissue of dental follicles and dentigerous cysts. Therefore, it would seem reasonable to discuss as whether odontogenic epithelium proliferations, frankly displaying ameloblastomatous features

  16. The expression of calretinin and cytokeratins in canine acanthomatous ameloblastoma and oral squamous cell carcinoma.

    Science.gov (United States)

    Fulton, A; Arzi, B; Murphy, B; Naydan, D K; Verstraete, F J M

    2014-12-01

    Oral squamous cell carcinoma (OSCC) and canine acanthomatous ameloblastoma (CAA) represent two epithelium-derived neoplasms that affect the oral cavity of dogs. The expression of cytokeratins (CKs) and calretinin has been previously established in the canine tooth bud and odontogenic tumours. The aim of this study was to characterize the CK and calretinin expression profile of OSCC in comparison to CAA and canine tooth bud tissues. Samples from 15 OSCC and 15 CAA cases, as well as 6 tooth buds and 2 normal gingival tissues were examined. OSCC CK expression was consistent with the CK expression profile of CAA and canine tooth bud tissue. Calretinin was positively expressed in 10 of 15 OSCC cases, with 5 cases demonstrating high staining intensity. Only 2 of 15 CAA cases demonstrated mild-moderate staining intensity. The statistically significant difference in staining pattern and intensity of calretinin in OSCC and CAA can help distinguish between these two tumour types. © 2012 Blackwell Publishing Ltd.

  17. Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas

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    Rogelio González-González

    2016-01-01

    Full Text Available Ameloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods have been developed for this purpose. The most widely used method is the labeling index, which has undergone changes over time to better facilitate cell counting. Here, we compared manual cell counting methods with automated cell counting (ImmunoRatio to determine the relative effectiveness of these methods. The results suggest that ImmunoRatio, a free software tool, may be highly advantageous and provide results similar to manual cell counting methods when used with the appropriate calibration. However, ImmunoRatio has flaws that may affect the labeling index results. Therefore, this automated cell counting method must be supplemented with manual cell counting methods.

  18. Immunoexpression of Ki-67, MCM2, and MCM3 in Ameloblastoma and Ameloblastic Carcinoma and Their Correlations with Clinical and Histopathological Patterns.

    Science.gov (United States)

    Carreón-Burciaga, Ramón Gil; González-González, Rogelio; Molina-Frechero, Nelly; Bologna-Molina, Ronell

    2015-01-01

    Cell proliferation assays are performed using antibodies against nuclear proteins associated with DNA replication. These nuclear proteins have gained special interest to predict the biological and clinical behaviors of various tumors. The aim of this study was to analyze the presence of Ki-67 protein and the minichromosome maintenance-2 (MCM2) and maintenance-3 (MCM3) proteins in ameloblastoma. Materials and Methods. Cell proliferation marker expression levels were assessed via immunohistochemistry in 111 ameloblastoma cases (72 unicystic ameloblastoma samples, 38 solid/multicystic ameloblastoma samples, and 1 ameloblastic carcinoma). The label index was performed as described previously. Results. MCM2 and MCM3 showed higher proliferation indexes in all variants of ameloblastoma compared to the classic marker Ki-67. No correlation between the proliferation index and the clinical and protein expression data was observed. Conclusion. The results suggest that clinical features do not directly affect tumor cell proliferation. Moreover, the high levels of cellular proliferation of MCM2 and MCM3 compared with Ki-67 may indicate that MCM2 and MCM3 are more sensitive markers for predicting the growth rate and eventually might be helpful as a tool for predicting aggressive and recurrent behaviors in these tumors.

  19. Peripheral ameloblastoma with histologically low-grade malignant features of the buccal mucosa: a case report with immunohistochemical study and genetic analysis.

    Science.gov (United States)

    Goda, Hiroyuki; Nakashiro, Kohichi; Ogawa, Ikuko; Takata, Takashi; Hamakawa, Hiroyuki

    2015-01-01

    Peripheral ameloblastoma (PA), a rare and unusual variant of odontogenic tumors, comprises about 1% of all ameloblastomas. PA is an exophytic growth localized to the soft tissues overlying the tooth-bearing areas of the jaws, and the initial diagnosis is often fibrous epulis. PA with histologically low-grade malignant features is extremely rare. We report a case of peripheral ameloblastoma with histologically low-grade malignant features in a 69-year-old woman that presented with a hemorrhage from a tumor on the right buccal mucosa. The tumor was surgically removed by blunt dissection, with no evidence of recurrence after two years and six months. After the case presentation, microscopic and genetic findings are discussed.

  20. Immunohistochemical Expression of GLUT-1 and HIF-1α in Tooth Germ, Ameloblastoma, and Ameloblastic Carcinoma.

    Science.gov (United States)

    Sánchez-Romero, Celeste; Bologna-Molina, Ronell; Mosqueda-Taylor, Adalberto; Paes de Almeida, Oslei

    2016-08-01

    Hypoxia-inducible factor-1α (HIF-1α) promotes proteins that enable cell survival during hypoxia, such as glucose transporter 1 (GLUT-1). Their coexpression has been associated with aggressiveness in malignancies and has not been studied in odontogenic tumors. Immunohistochemical expression of HIF-1α and GLUT-1 was analyzed in 13 tooth germs (TGs), 55 ameloblastomas (AMs), and 3 ameloblastic carcinomas (ACs). HIF-1α was negative in all TGs, and just 1 case of AM and 1 of AC had nuclear positivity. GLUT-1 expressed in ameloblastic cells of all TGs, AMs, and ACs, with an increasing intensity, respectively, and was significantly higher in solid AM than in unicystic AM (P = .041). Absence of nuclear HIF-1α in TGs and most AMs suggest that GLUT-1 may be induced by alternative pathways to hypoxia. However, in ACs, HIF-1α may be activated; however, to confirm this, additional cases are needed. GLUT-1 overexpression could be related to aggressiveness in AMs and ACs and must represent a normal metabolite in TGs. © The Author(s) 2016.

  1. Glandular odontogenic cyst mimicking ameloblastoma in a 78 year old female: A case report

    International Nuclear Information System (INIS)

    Lee, Byung Do; Lee, Wan; Kwon, Kyung Hwan; Choi, Moon Ki; Choi, Eun Joo; Yoon, Jung Hoon

    2014-01-01

    Glandular odontogenic cyst (GOC) is a rare, potentially aggressive jaw lesion. The common radiographic features include a well-defined radiolucency with distinct borders, presenting a uni- or multilocular appearance. A cystic lesion in the posterior mandible of a 78-year-old female was incidentally found. Radiographs showed a unilocular lesion with a scalloped margin, external root resorption of the adjacent tooth, and cortical perforation. This lesion had changed from a small ovoid shape to a more expanded lesion in a period of four years. The small lesion showed unilocularity with a smooth margin and a well-defined border, but the expanded lesion produced cortical perforation and a lobulated margin. The provisional diagnosis was an ameloblastoma, whereas the histopathological examination revealed a GOC. This was a quite rare case, given that this radiographic change was observed in the posterior mandible of an elderly female. This case showed that a GOC can grow even in people in their seventies, changing from the unilocular form to an expanded, lobulated lesion. Here, we report a case of GOC with characteristic radiographic features.

  2. Glandular odontogenic cyst mimicking ameloblastoma in a 78 year old female: A case report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Byung Do; Lee, Wan; Kwon, Kyung Hwan; Choi, Moon Ki; Choi, Eun Joo [College of Dentistry, Wonkwang University, Iksan (Korea, Republic of); Yoon, Jung Hoon [Dept. of Oral and Maxillofacial Pathology, College of Dentistry, Daejeon Dental Hospital, Wonkwang University, Daejeon (Korea, Republic of)

    2014-09-15

    Glandular odontogenic cyst (GOC) is a rare, potentially aggressive jaw lesion. The common radiographic features include a well-defined radiolucency with distinct borders, presenting a uni- or multilocular appearance. A cystic lesion in the posterior mandible of a 78-year-old female was incidentally found. Radiographs showed a unilocular lesion with a scalloped margin, external root resorption of the adjacent tooth, and cortical perforation. This lesion had changed from a small ovoid shape to a more expanded lesion in a period of four years. The small lesion showed unilocularity with a smooth margin and a well-defined border, but the expanded lesion produced cortical perforation and a lobulated margin. The provisional diagnosis was an ameloblastoma, whereas the histopathological examination revealed a GOC. This was a quite rare case, given that this radiographic change was observed in the posterior mandible of an elderly female. This case showed that a GOC can grow even in people in their seventies, changing from the unilocular form to an expanded, lobulated lesion. Here, we report a case of GOC with characteristic radiographic features.

  3. Enriched vascularity in ameloblastomas, an indeterminate entity: Report of two cases

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    Usha Hegde

    2015-01-01

    Full Text Available Vascularity is a highly essential element that is required for the growth, development, and functioning of the body and variations in it can cause pathologies. It is one of the prime features of a proliferating lesion, where it aids in the growth of the lesion through its nutrition supply. Highly increased vascularity in a disease can itself affect the prognosis of the lesion, and in malignancies, it can induce tumor seeding and secondaries. Most of the pathologies including tumors, related to blood vessels, and vascularity are well established. There are some conditions, wherein altered vascularity is one of the prime components along with other diagnostic components of an established disease. In such cases, these lesions are diagnosed with special names, with varying biological behavior and prognosis in comparison to that of established entity. However, there still are few similar conditions whose nature is uncertain due to the rarity of the lesion and the insufficient scientific evidence which eludes the diagnostician. Here is the report of two cases of ameloblastoma, an established entity, with significant vascularity whose nature is indeterminate.

  4. Achieving Adequate Margins in Ameloblastoma Resection: The Role for Intra-Operative Specimen Imaging. Clinical Report and Systematic Review

    Science.gov (United States)

    Ramakrishnan, Anand; Mirkazemi, Mansoor; Baillieu, Charles; Ptasznik, Ronnie; Leong, James

    2012-01-01

    Background Ameloblastoma is a locally aggressive odontogenic neoplasm. With local recurrence rates reaching 90%, only completeness of excision can facilitate cure. Surgical clearance has widely been based on pre-operative imaging to guide operative excision margins, however use of intra-operative specimen x-ray or frozen-section has been sought to improve clearance rates, and advanced imaging technologies in this role have been proposed. This manuscript aims to quantify the evidence for evaluating intra-operative resection margins and present the current standard in this role. Method The current study comprises the first reported comparison of imaging modalities for assessing ameloblastoma margins. A case is presented in which margins are assessed with each of clinical assessment based on preoperative imaging, intra-operative specimen x-ray, intra-operative specimen computed tomography (CT) and definitive histology. Each modality is compared quantitatively. These results are compared to the literature through means of systematic review of current evidence. Results A comparative study highlights the role for CT imaging over plain radiography. With no other comparative studies and a paucity of high level evidence establishing a role for intra-operative margin assessment in ameloblastoma in the literature, only level 4 evidence supporting the use of frozen section and specimen x-ray, and only one level 4 study assesses intra-operative CT. Conclusion The current study suggests that intra-operative specimen CT offers an improvement over existing techniques in this role. While establishing a gold-standard will require higher level comparative studies, the use of intra-operative CT can facilitate accurate single-stage resection. PMID:23094099

  5. Achieving adequate margins in ameloblastoma resection: the role for intra-operative specimen imaging. Clinical report and systematic review.

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    Inoka De Silva

    Full Text Available BACKGROUND: Ameloblastoma is a locally aggressive odontogenic neoplasm. With local recurrence rates reaching 90%, only completeness of excision can facilitate cure. Surgical clearance has widely been based on pre-operative imaging to guide operative excision margins, however use of intra-operative specimen x-ray or frozen-section has been sought to improve clearance rates, and advanced imaging technologies in this role have been proposed. This manuscript aims to quantify the evidence for evaluating intra-operative resection margins and present the current standard in this role. METHOD: The current study comprises the first reported comparison of imaging modalities for assessing ameloblastoma margins. A case is presented in which margins are assessed with each of clinical assessment based on preoperative imaging, intra-operative specimen x-ray, intra-operative specimen computed tomography (CT and definitive histology. Each modality is compared quantitatively. These results are compared to the literature through means of systematic review of current evidence. RESULTS: A comparative study highlights the role for CT imaging over plain radiography. With no other comparative studies and a paucity of high level evidence establishing a role for intra-operative margin assessment in ameloblastoma in the literature, only level 4 evidence supporting the use of frozen section and specimen x-ray, and only one level 4 study assesses intra-operative CT. CONCLUSION: The current study suggests that intra-operative specimen CT offers an improvement over existing techniques in this role. While establishing a gold-standard will require higher level comparative studies, the use of intra-operative CT can facilitate accurate single-stage resection.

  6. Podoplanin, E-cadherin, β-catenin, and CD44v6 in recurrent ameloblastoma: their distribution patterns and relevance.

    Science.gov (United States)

    Siar, Chong Huat; Ishak, Ismadi; Ng, Kok Han

    2015-01-01

    Ameloblastoma is a benign but locally infiltrative odontogenic epithelial neoplasm with a high risk for recurrence. Podoplanin, a lymphatic endothelium marker, putatively promotes collective cell migration and invasiveness in this neoplasm. However, its role in the recurrent ameloblastoma (RA) remains unclear. As morphological, signaling, and genetic differences may exist between primary and recurrent tumors, clarification of their distribution patterns is of relevance. Podoplanin was examined immunohistochemically in conjunction with E-cadherin, β-catenin, and CD44v6 in 25 RA. Immunostaining according to tumor area, cellular type, and location, and relationship of these proteins were analyzed. Findings were compared with 25 unrelated primary ameloblastomas (UPA). All four proteins were detected in RA and UPA samples. Expression rates for each protein were not significantly different between these two groups. RA demonstrated significant upregulation of podoplanin at the invasive front (P 0.05). Immunolocalization for all four proteins was predominantly membranous and less frequently cytoplasmic. Pre-ameloblast-like cells were podoplanin(+) /CD44v6(-), while stellate reticulum-like cells were podoplanin(-)/CD44v6(+). Acanthomatous, granular cell, and desmoplastic variants in both RA and UPA were podoplanin(-/low) but stained weak-to-moderate for E-cadherin, β-catenin, and CD44v6. Stromal fibroblasts and lymph channels were variably podoplanin-positive. Podoplanin, β-catenin, and CD44v6 upregulation at the tumor invasive fronts in RA and UPA supports a differential regulatory role by these molecules in mediating collective cell migration and local invasiveness. E-cadherin downregulation suggests altered cell adhesion function during tumor progression. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  7. Estudo retrospectivo dos casos diagnosticados como ameloblastoma no Serviço de Anatomia Patológica do Hospital Universitário Antônio Pedro entre 1997 e 2007 A retrospective study of ameloblastoma in the Anatomic Pathology Service of Antônio Pedro University Hospital between 1997 and 2007

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    Daniela Otero Pereira da Costa

    2008-12-01

    Full Text Available INTRODUÇÃO: O ameloblastoma é um tumor odontogênico de origem epitelial que, pela freqüência, subtipos clínicos, grande capacidade infiltrativa e alta tendência à recorrência tem merecido bastante atenção. OBJETIVO:Realizar estudo retrospectivo dos casos diagnosticados histopatologicamente de ameloblastoma no Serviço de Anatomia Patológica do Hospital Universitário Antônio Pedro da Universidade Federal Fluminense (HUAP/UFF no período de 1997 a 2007. Material e métodos: Foram selecionados casos de ameloblastoma correspondentes ao período da pesquisa, e dados como idade, sexo, raça, localização da lesão, aspecto radiográfico, tipo de biópsia, diagnósticos clínico e histopatológico foram compilados das fichas de requisição e/ou prontuários. RESULTADOS:Depois do levantamento e da revisão das lâminas para confirmação do diagnóstico de ameloblastoma, a amostra totalizada foi de 23 casos. Os pacientes mais acometidos eram do sexo masculino, leucodermas e estavam entre a primeira e a terceira década de vida. Todos os casos ocorreram na mandíbula, sendo a região posterior a mais afetada. Predominaram o aspecto radiográfico multilocular e, histopatologicamente, o padrão multicístico, sendo os subtipos folicular e híbrido os mais freqüentes. Não foram observados casos de ameloblastoma desmoplásico e de células basais. CONCLUSÕES: A freqüência do ameloblastoma varia de acordo com a região geográfica avaliada, e a realização de estudos retrospectivos pode contribuir para a identificação de grupos e dos possíveis fatores associados ao seu desenvolvimento.BACKGROUND: Ameloblastoma is an odontogenic tumor of epithelial origin that has received considerable attention due to its frequency, clinical subtypes and high tendency to infiltrate and recur. OBJECTIVE: To perform a retrospective study of cases histopathologically diagnosed of ameloblastoma at the Pathological Anatomy Department of APUH/UFF-RJ from

  8. Reconstruction of Temporal Hollowing Defect With Anterior-Lateral Thigh Free Flap Following Resection of Recurrent Ameloblastoma of the Infratemporal Fossa and Right Mandible (a Case Report).

    Science.gov (United States)

    Melville, James C; Hornberger, John C; Young, Simon; Shum, Jonathan W

    2016-09-01

    Recurrent invasive ameloblastoma of the infratemporal fossa is an uncommonly encountered phenomenon in the practice of oral and maxillofacial surgery and presents many surgical challenges for the practitioner. This case report describes a patient who underwent previous resection of a mandibular ameloblastoma with multiple recurrences. The patient was diagnosed with a recurrent ameloblastoma of the infratemporal fossa that was subsequently resected and reconstructed using an anterolateral thigh (ALT) free tissue transfer. There are few reported cases of recurrent ameloblastomas in the infratemporal fossa and none that describe surgical resection and reconstruction of such a lesion. Owing to the uniqueness of the surgical defect, an ALT flap was used to correct the temporal hollowing. There have been multiple reported cases of reconstruction of temporal hollowing defects using autogenous fat or allograft; however, none have described the use of a de-epithelialized ALT microvascular reconstruction of a temporal hollowing defect. This case report describes a unique clinical situation of surgical resection and reconstruction that resulted in a satisfactory outcome for the patient. Copyright © 2016 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  9. Ameloblastoma desmoplásico con predominio quístico: Presentación de un caso clínico

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    Begoña Martínez-Revilla

    2016-07-01

    Full Text Available El ameloblastoma es una neoplasia odontogénica que muestra un comportamiento agresivo local. Algunas variantes como la uniquística y la desmoplásica tienen un mejor pronóstico que la sólida/multiquística. Presentamos un caso en un varón de 76 años con una lesión radiolúcida interradicular mandibular de apariencia quística, la cual se enucleó quirúrgicamente. Histológicamente correspondió a una lesión desmoplásica con una amplia zona quística que fue diagnosticada como ameloblastoma desmoplásico con predominio quístico. Trascurridos 30 meses no se evidencia recidiva. En este artículo se discuten los principales aspectos clinicopatológicos de estas entidades.

  10. Relative expression of α-smooth muscle actin and matrix metalloproteinases-2 in ameloblastoma of a black African sub-population.

    Science.gov (United States)

    Adisa, Akinyele O; Udeabor, Samuel E; Adeyemi, Bukola F; Alica, Kubesch; Booms, Patrick; Ghanaati, Shahram; Sader, Robert A

    2015-01-01

    Ameloblastoma although a benign odontogenic tumor, is locally invasive. The abundant presence of myofibroblasts (marked by α-smooth muscle actin [α-SMA]) in the stroma and expression of matrix metalloproteinase-2 (MMP-2) in the neoplastic or stromal cells have been linked with the tumor's ability for both local and distant spread. We aim to estimate the relative expression of α-SMA and MMP-2 in ameloblastoma from a black African subgroup to gauge their relative potential for enhancing local invasiveness and hence, their prospects as possible chemotherapeutic targets. Twenty-five formalin-fixed paraffin-embedded blocks of ameloblastoma cases from Nigeria were prepared for antibody processing to α-SMA (Dako Monoclonal Mouse Anti-Human α-SMA antibody clone 1A4) and MMP-2 (Abcam Mouse Monoclonal Anti-MMP-2 antibody [CA-4001/CA719E3C] ab3158). The score for percentage positivity of the tumor cells and the score for staining intensities were then multiplied in order to generate an immunoreactive score. α-smooth muscle actin was only expressed in the fibrous connective tissues adjacent to the tumor islands while MMP-2 was expressed in the ameloblasts, stellate reticulum, and the connective tissues in varying proportions. All the variants analyzed expressed α-SMA mildly or moderately, except for the follicular variant that either did not express α-SMA or expressed it mildly. The highest number of strong immunoreactivity to MMP-2 in the ameloblast region was found in the plexiform variant. Chemotherapeutic targeting of both molecules may, therefore, be a vital step in the control of local ameloblastoma invasiveness.

  11. Rare copy number alterations and copy-neutral loss of heterozygosity revealed in ameloblastomas by high-density whole-genome microarray analysis

    DEFF Research Database (Denmark)

    Diniz, Marina Gonçalves; Duarte, Alessandra Pires; Villacis, Rolando A

    2017-01-01

    , and PPP2R5A) covered by rare alterations, also including three MA and four normal oral tissues. RESULTS: Fifty-seven CNAs and cnLOH were observed in the ameloblastomas and six CNAs in the AC. Seven of the CNAs were rare (six in UA and one in MA), four of them encompassing genes (gains of 7q11.21, 1q32...

  12. Educational effect of a lecture on differential imaging features comparing ameloblastomas and keratocystic odontogenic tumors of the mandible presented to dental students

    International Nuclear Information System (INIS)

    Morita, Mitsuko; Ariji, Yoshiko; Kise, Yoshitaka; Goto, Masakazu; Izumi, Masahiro; Naitoh, Munetaka; Ariji, Eiichiro; Katsumata, Akitoshi

    2011-01-01

    The objective of this study was to clarify the educational effect of a lecture on differential imaging features comparing ameloblastomas and keratocystic odontogenic tumors of the mandibles presented to dental students. Panoramic and CT images of 10 ameloblastomas and 10 keratocystic odontogenic tumors were randomly presented 114 dental students. Test scores, correct answer ratios, identification index, and understanding of the imaging features contributing to a correct diagnosis were serially evaluated before and after the lecture on the differential imaging features comparing the two types of tumors. The mean and standard deviation of the scoring ratios of dental students diagnosing these lesions on panoramic and CT images were 48.8±10.8% and 52.5±12.9%, respectively. After the lecture on the differential imaging features comparing the two tumors, the scoring ratios improved significantly. After the lecture, both the numbers of patients whose images were correctly diagnosed and the identification indices increased. The lecture also increased the number of imaging features recognized as contributing to the correct diagnosis. A lecture on the differential imaging features comparing ameloblastomas and keratocystic odontogenic tumors of the mandibles contributed to the improvement of imaging diagnosis skills among dental students. (author)

  13. Assessment of quality of life in giant ameloblastoma adolescent patients who have had mandible defects reconstructed with a free fibula flap.

    Science.gov (United States)

    Li, Xiangru; Zhu, Kuicheng; Liu, Fei; Li, Hongwen

    2014-07-08

    The reconstruction of mandibular defects after giant ameloblastoma resection is one of the most challenging problems facing reconstructive surgeons. Mandibular resection has been associated with a poor quality of life (QOL), particularly in adolescent patients reconstructed with a free fibula flap. This study aims to evaluate QOL outcomes in adolescent patients who have had mandibular resections of giant ameloblastoma and reconstruction with a free fibula flap and to collect information about their socio-cultural situation. The present study assessed 45 adolescent patients who had undergone immediate mandible reconstruction with a free fibula flap for faint ameloblastoma using University of Washington Quality of Life (UW-QOL) and 14-item Oral Health Impact Profile (OHIP-14) questionnaires. Thirty-five of the 54 questionnaires were returned (64.81%). In the UW-QOL, of the twelve disease-specific domains, the best three scores from the patients related to pain, shoulder and appearance and the worst three scores related to chewing, anxiety and saliva. In the OHIP-14, the lowest-scoring domain was handicap, followed by physical pain and social disability. Mandibular reconstruction with a free fibular flap significantly influenced the adolescent patients' QOL. Adolescent patients pay more attention to postoperative facial appearance; this should be considered in surgical planning.

  14. Off-label use of rhBMP-2 as bone regeneration strategies in mandibular ameloblastoma unicystic.

    Science.gov (United States)

    Silva, Henrique Celestino Lima E; Cheim, Adonai Peixoto; Moreno, Roberto; Miranda, Sérgio Luis de

    2017-01-01

    Jawbone reconstruction after tumor resection is one of the most challenging clinical tasks for maxillofacial surgeons. Osteogenic, osteoinductive, osteoconductive and non-antigenic properties of autogenous bone place this bone as the gold standard for solving problems of bone availability. However, the need for a second surgical site to harvest the bone graft increases significantly both the cost and the morbidity associated with the reconstructive procedures. Bone grafting gained an important tool with the discovery of bone morphogenetic proteins in 1960. Benefit of obtaining functional and real bone matrix without need of second surgical site seems to be the great advantage of use bone morphogenetic proteins. This study analyzed the use of rhBMP-2 in unicystic ameloblastoma of the mandible, detailing its structure, mechanisms of cell signaling and biological efficacy, in addition to present possible advantages and disadvantages of clinical use of rhBMP-2 as bone regeneration strategy. RESUMO A reconstrução óssea dos maxilares após ressecções tumorais é uma das tarefas mais difíceis para o cirurgião maxilofacial. As propriedades osteogênicas, osteoindutoras, osteocondutoras e não antigênicas do osso autógeno o colocam como o padrão-ouro para a solução de problemas de disponibilidade óssea. Entretanto a coleta do enxerto ósseo necessita de um segundo sítio cirúrgico, aumentando significativamente o custo e a morbidade associados ao procedimento reconstrutivo. A enxertia óssea ganhou uma excelente ferramenta com a descoberta das proteínas ósseas morfogenéticas na década de 1960. O benefício da obtenção de matriz óssea verdadeira e funcional, sem a necessidade de um segundo sítio cirúrgico, parece ser a grande vantagem do uso das proteínas ósseas morfogenéticas. Neste contexto, o objetivo deste estudo foi analisar a utilização da rhBMP-2 na regeneração óssea de ameloblastoma mandibular unicístico, detalhando sua estrutura, seus

  15. Unsuspected small ameloblastoma in the alveolar bone: a collaborative study of 14 cases with discussion of their cellular sources.

    Science.gov (United States)

    Ide, F; Mishima, K; Yamada, H; Horie, N; Saito, I; Shimoyama, T; Kusama, K

    2008-04-01

    Intraosseous ameloblastoma (IA) is the quintessence of epithelial odontogenic tumor and histologically and behaviorally defined as an undoubted neoplastic process. Current information must lead to the consensus that IA arises from the embryologic inclusions of odontogenic epithelium within the jawbone. Nevertheless, clinically oriented evidence is limited to this day. The clinical and radiographic features, behavior, and pathology of 14 cases of small IA confined to the alveolar region were systematically examined. Six cases were a chance finding. There was no gender predilection and half of the lesions clustered in middle age (>40 years). The posterior region of the mandible (n = 7) and the anterior segment of the maxilla (n = 4) were favored. Five radiographic characteristics were recognized: interradicular (n = 5) and periradicular (n = 3), and periapical, residual and pericoronal (n = 2 each). They showed solid (n = 12) or unicystic (n = 2) growth pattern and 12 lesions were divided into seven follicular, three desmoplastic, and two plexiform subtypes. The main location of tumor was microscopically traceable in six cases; three interradicular type outside the periodontal ligament space and two periradicular and one periapical variants inside. By in-depth evaluation of the spatial relationship between tumor and its surrounding structure, the alveolar process, periodontal ligament space, and pericoronal area are all the likely starting points of IA. This report re-awakens the oral pathologist to the histogenetic significance of incipient IA as the only available human specimen for reappraisal of their origin.

  16. Expression of TP53 and IL-1α in unicystic ameloblastoma predicts the efficacy of marsupialization treatment.

    Science.gov (United States)

    Zhang, Xinyu; Liu, Liu; Yang, Xi; Wang, Lizhen; Zhang, Chenping; Hu, Yongjie

    2018-02-01

    In this study, we evaluated the effects of marsupialization in treating unicystic ameloblastoma (UA) and investigated the relationship between TP53 and interleukin 1 α (IL-1α) expression and the clinical outcome of UA treated with marsupialization.Consecutive patients treated with marsupialization and curettage at Shanghai Ninth People's Hospital were included. According to the unified standard, 48 patients were included in this study. Of these, 20 showed a good response, 10 a partial response, and 18 no response, based on the outcome of the marsupialization procedure. The expression of proteins TP53 and IL-1α was detected with immunohistochemistry (IHC). The clinical and pathological characteristics of the patients were analyzed.Analysis of the clinical and pathological characteristics showed that the effects of marsupialization treatment were significantly associated with lesion location (P < .001) and tumor diameter (P = .01). IHC showed that TP53 expression was significantly higher in the good-response group than in the partial- or no-response group (P = .02), and IL-1α expression was significantly higher in the good-response group than in the partial- and no-response groups (P = .03).Marsupialization is an effective preliminary procedure for treating UA before curettage and peripheral ostectomy. The expression of the TP53 and IL-1α proteins correlates directly with the outcome of UA treated with marsupialization.

  17. Spontaneous multi-cystic peripheral ameloblastoma in the freshwater angelfish, from the Brazilian state of Pará

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    M. Videira

    2015-08-01

    Full Text Available Este trabalho registra a ocorrência espontânea de ameloblastoma em P. scalare. O tumor foi obtido a partir de um exemplar de Acará Bandeira, sendo fixado, seccionado, e os fragmentos processados para microscopia de luz e microscopia eletrônica de varredura (MEV. O exame macroscópico evidenciou uma massa tumoral que se estendia do rebordo alveolar do maxilar superior à face externa labial. À MEV, a neoplasia apresentou uma série de espículas. Microscopicamente, percebia-se um processo neoplásico constituído por tecido conjuntivo de característica mixoide, ricamente vascularizado, onde eram observados fragmentos de tecido osteoide. A amostra revelou proliferação neoplásica do epitélio odontogênico, onde as células neoplásicas se organizavam na forma de paliçada. Alterações histopatológicas em peixes têm sido úteis biomarcadores do efeito à exposição a substâncias tóxicas, sendo as neoplasias lesões específicas, comumente encontradas em peixes de áreas poluídas, revelando uma associação entre as lesões e a exposição a irritantes.

  18. TNF-α-induced IL-6 and MMP-9 expression in immortalized ameloblastoma cell line established by hTERT.

    Science.gov (United States)

    Ohta, K; Naruse, T; Ishida, Y; Shigeishi, H; Nakagawa, T; Fukui, A; Nishi, H; Sasaki, K; Ogawa, I; Takechi, M

    2017-03-01

    Ameloblastoma (AM) shows locally invasive behaviour. However, biological investigations regarding regulation of gene expression associated with AM pathological features are difficult to perform, because AM cells can be passaged for a few generations due to senescence. We report a newly established immortalized AM cell line, AMB cells, by transfection with human telomerase reverse transcriptase (hTERT). Furthermore, we examined whether TNF-α modulates bone resorption-related genes, IL-6 and MMP-9 in cooperation with TGF-β or IFN-γ. Following transfection of an hTERT expression vector into AM cells using a non-viral method, the effects of cytokines on the expressions of IL-6 and MMP-9 mRNA were examined using real-time PCR. TNF-α-induced NF-κB activity was examined by western blotting and transcription factor assays. AMB cells continued to grow for more than 100 population doublings. Stimulation with TNF-α increased IL-6 and MMP-9 mRNA expressions, as well as NF-κB activation. Furthermore, TGF-β and IFN-γ dramatically increased TNF-α-mediated expressions of MMP-9 and IL-6 mRNA, respectively, while those responses were suppressed by NF-κB inhibitor. We established an immortalized AM cell line by hTERT transfection. TNF-α-mediated regulation of MMP-9 and IL-6 via NF-κB may play an important role in the pathological behaviour of AMs, such as bone resorption. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  19. 3D cone beam computed tomography reconstruction images in diagnosis of ameloblastomas of lower jaw: A case report and mini review.

    Science.gov (United States)

    Li, Dize; Yang, Zhiqiang; Chen, Tao; Guan, Chao; Wang, Feilong; Matz, Ethan L; Zhang, Yuanyuan; Ji, Ping

    2018-01-01

    Cone beam computed tomography (CBCT) has obvious advantages over regular radiography in diagnosis of complex diseases. Objective of this study is to report a case of a mandibular jaw ameloblastoma recurring cyst, which represents a benign tumor of odontogenic epithelium, using CBCT imaging technology. CBCT examination of the patient suffering with recurrent lower jaw cyst (relapsing four years after surgery) showed a decrease in irregular bone density and appearance of a honeycomb pattern (3.5 cm×2.5 cm×1.8 cm) in the right lower jaw. This suggests that the lesion is more likely to be an ameloblastoma. Preoperative tissue biopsy and pathological examination of surgical sample confirmed the diagnosis. Surgical resection of the diseased tissue and autogenous bone grafting in the mandible was performed. Postoperative CBCT examination showed that the bone defect healed well, without recurrence of the tumor 22 months postoperatively. In conclusion, the rotated 3D CBCT images clearly displays the exact size, location, borders and internal changes of the tumor in the jaw cyst itself and the adjacent tissues. Thus, the dental CBCT allows clinicians to better evaluate lesions, leading to better treatment outcomes.

  20. Comparison of immunoexpression of VEGF, TGF-β and MMP-9 in ameloblastoma and adenomatoid odontogenic tumor = Comparação da imunoexpressão de VEGF, TGF-β e MMP-9 em ameloblastoma e tumor odontogênico adenomatóide

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    Ferreira, Stefânia Jeronimo

    2015-01-01

    Full Text Available Objetivo: Estudos sobre tumores odontogênicos têm identificado várias disfunções moleculares envolvidas no seu desenvolvimento, e alguns mecanismos como a angiogênese e modulação da matriz são objetos úteis para investigar as diferenças no comportamento biológico destes tumores. Alguns marcadores importantes para identificar a agressividade do tumor por imunoistoquímica são as proteínas VEGF, TGF-ß e MMP-9. Este estudo teve como objetivo comparar a expressão imunoistoquímica de VEGF, TGF-ß e MMP-9 entre ameloblastoma e tumor odontogênico adenomatoide (TOA. Métodos: Imunoexpressão de VEGF, TGF-ß e MMP-9 foi estudada em 15 ameloblastomas sólidos e 15 TOA. Uma análise semiquantitativa das células imunomarcadas foi realizada e a análise estatística foi feita usando o teste não paramétrico de Mann-Whitney e o teste de correlação de Spearman, com nível de significância de 0,05 (P0. 05. Conclusão: Os resultados sugerem o envolvimento da angiogênese na progressão tumoral de ameloblastomas e o efeito indutor de células estromais em TOA, portanto, justificando o seu potencial de crescimento mais baixo

  1. Tratamento cirúrgico do ameloblastoma com reconstrução de mandíbula com enxerto de crista ilíaca não vascularizado: estudo de sete casos

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    Daniela Venturoli Lunardi

    Full Text Available OBJETIVO: Determinar a validade do enxerto autólogo de crista ilíaca não vascularizado no tratamento cirúrgico do ameloblastoma de mandíbula. MÉTODO: Nos Serviços de Cirurgia de Cabeça e Pescoço e Semiologia Bucal do Complexo Hospitalar Heliópolis, de 1980 a 2000, foram tratados 31 pacientes com ameloblastoma de mandíbula, dos quais sete receberam enxerto de crista ilíaca autólogo, fixos com placa de titânio do sistema A-0 (quatro casos e aço inox (três casos, sendo portadores da variedade folicular (seis casos e plexiforme (um caso. RESULTADOS: Nesta análise, foi utilizado o Teste de Hipótese para a média populacional com a variança desconhecida, houve exposição da placa em três casos (40% quando a neoplasia ultrapassava a linha média e em quatro casos (60% não incidiu nenhuma complicação. CONCLUSÕES: Apesar da incidência de exposição de placa, o método é indicado na reconstrução da mandíbula de pacientes com ameloblastoma.

  2. Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells.

    Science.gov (United States)

    Jiang, Chunmiao; Zhang, Qunzhou; Shanti, Rabie M; Shi, Shihong; Chang, Ting-Han; Carrasco, Lee; Alawi, Faizan; Le, Anh D

    2017-09-01

    Epithelial-mesenchymal transition (EMT), a biological process associated with cancer stem-like or cancer-initiating cell formation, contributes to the invasiveness, metastasis, drug resistance, and recurrence of the malignant tumors; it remains to be determined whether similar processes contribute to the pathogenesis and progression of ameloblastoma (AM), a benign but locally invasive odontogenic neoplasm. Here, we demonstrated that EMT- and stem cell-related genes were expressed in the epithelial islands of the most common histologic variant subtype, the follicular AM. Our results revealed elevated interleukin (IL)-6 signals that were differentially expressed in the stromal compartment of the follicular AM. To explore the stromal effect on tumor pathogenesis, we isolated and characterized both mesenchymal stromal cells (AM-MSCs) and epithelial cells (AM-EpiCs) from follicular AM and demonstrated that, in in vitro culture, AM-MSCs secreted a significantly higher level of IL-6 as compared to the counterpart AM-EpiCs. Furthermore, both in vitro and in vivo studies revealed that exogenous and AM-MSC-derived IL-6 induced the expression of EMT- and stem cell-related genes in AM-EpiCs, whereas such effects were significantly abrogated either by a specific inhibitor of STAT3 or ERK1/2, or by knockdown of Slug gene expression. These findings suggest that AM-MSC-derived IL-6 promotes tumor-stem like cell formation by inducing EMT process in AM-EpiCs through STAT3 and ERK1/2-mediated signaling pathways, implying a role in the etiology and progression of the benign but locally invasive neoplasm. Stem Cells 2017;35:2083-2094. © 2017 AlphaMed Press.

  3. Role of hypoxia-related proteins in invasion of ameloblastoma cells: crosstalk between NOTCH1, hypoxia-inducible factor 1α, a disintegrin and metalloproteinase 12, and heparin-binding epidermal growth factor.

    Science.gov (United States)

    da Costa, Natacha Malu Miranda; Fialho, Amanda Dalla Vechia; Proietti, Carolina Carmine; da Silva Kataoka, Maria Sueli; Jaeger, Ruy Gastaldoni; de Alves-Júnior, Sérgio Melo; de Jesus Viana Pinheiro, João

    2016-07-01

    Ameloblastoma AME is a benign tumour characterized by local invasiveness, high recurrence rates, and diverse histological patterns. The oxygen concentration is reduced in specific areas of the tumour microenvironment, which leads to intratumoral hypoxia. Crosstalk between NOTCH1, a disintegrin and metalloproteinase 12 (ADAM-12), hypoxia-inducible factor 1α (HIF-1α) and heparin-binding epidermal growth factor (HB-EGF) under hypoxic conditions has been implicated in invadopodia formation, tumour invasiveness, and metastasis development. The aim of this study was to analyse the expression of these proteins, in order to further elucidate the mechanisms underlying AME invasiveness. Twenty cases of AME, eight calcifying cystic odontogenic tumours CCOTs and 10 samples of dental follicle were used to investigate the expression of these proteins by immunohistochemistry with the primary antibodies anti-NOTCH1, anti-ADAM-12, anti-HIF-1α, and anti-HB-EGF. Immunostaining results were expressed as the percentage of stained area in images acquired in an AxioScope microscope equipped with an AxioCamHRc camera and a × 40 objective. The results showed that immunoexpression of all proteins was higher in the AME samples than in the CCOT and dental follicle samples (P < 0.05). AME showed an increased presence of proteins associated with tumour invasiveness, which indicates a possible role of these proteins in the biological behaviour of this tumour. © 2015 John Wiley & Sons Ltd.

  4. Tumor odontogênico cístico calcificante com proliferação ameloblastomosa em seio maxilar Calcifying cystic odontogenic tumor with ameloblastoma proliferation in the maxillary sinus

    Directory of Open Access Journals (Sweden)

    Maria Carolina Gonçalves Carnasciali

    2012-08-01

    Full Text Available O tumor odontogênico cístico calcificante (TOCC com proliferação ameloblastomosa é uma variante rara entre os cistos maxilares. Este trabalho objetiva apresentar o relato clínico de um paciente do sexo masculino, 18 anos de idade, que apresentava aumento de volume extra e intraoral do lado esquerdo da maxila, firme à palpação, de característica normocrômica e indolor. A conduta consistiu em realização de tomografia Cone Beam, biópsia incisional, remoção completa da lesão, curetagem e fixação maxilar. O paciente encontra-se em acompanhamento clínico e radiográfico sem recidiva após doze meses. Dessa forma, ressalta-se a importância do diagnóstico precoce, a conduta clínica empregada e o acompanhamento periódico.Calcifying cystic odontogenic tumors (CCOT with proliferative ameloblastoma are a rare variant among maxillary cysts. This study aims to present a clinical report of an 18-year-old male patient with extra and intra oral swelling of the left maxilla, firm to touch, with normochromic characteristics and painless. The clinical approach comprised cone-beam tomography, incisional biopsy, complete removal of the lesion, curettage and maxilla fixation. His clinical and radiographic follow-up has revealed no relapse after 12 months. Hence, this study corroborates the importance of early diagnosis, clinical approach and periodical follow-ups.

  5. R eprodu ced by Sabin et G atew ay u n der licen ce gran ted by th e ...

    African Journals Online (AJOL)

    A.meloblastoma was the most commonlyseen odontogenic,tumour (73.7%), followeq by odontogefll~. myxoma (10.3%). The site, sex, and histologic distribution of ameloblastoma did not differ from other,. African studies. Over 50% of patients with ameloblastoma preseritep.to hospital late (after three years or more years).

  6. R eprodu ced by Sabin et G atew ay u n der licen ce gran ted by th e ...

    African Journals Online (AJOL)

    World-wide, ameloblastoma have been discussed in relation and modalities of treatment. In Tanzania ... Dentistry, Muhimbili University College of Health Sciences, were examined for all cases of oral and maxillofacial tumours ... The ameloblastoma cases were analysed for age, sex, anatomical distribution, clinical features,.

  7. A standardized formula for aesthetic mandibular reconstruction ...

    African Journals Online (AJOL)

    Ameloblastoma is the most common benign odontogenic tumor of the jaw, and expansional growth of a huge untreated ameloblastoma can result in disturbances in facial aesthetics and function, such as difficulty with mouth opening, swallowing, chewing, breathing, neurologic deficits, and pathologic fractures. Radical wide ...

  8. Author Details

    African Journals Online (AJOL)

    Biologic, anatomic and clinical considerations in the management of the classic interaosseous ameloblastoma of the jaws. Abstract · Vol 22, No 1 (2012) - Articles A Classification System for Recurrent Ameloblastoma of the Jaws- Review of 30 Cases in Nigerians Abstract. ISSN: 0189-2657. AJOL African Journals Online.

  9. An atypical adenomatoid odontogenic tumour in the mandible: a ...

    African Journals Online (AJOL)

    The differential diagnosis between AOT and other odontogenic tumours such as ameloblastoma should be well made to avoid extensive ablative surgery. However, AOT frequently resembles other odontogenic lesions such as dentigerous cysts or ameloblastoma. Immunohistochemically, AOT is characterized by positive ...

  10. Alpha-smooth muscle actin within epithelial islands is predictive of ameloblastic carcinoma.

    NARCIS (Netherlands)

    Bello, I.O.; Alanen, K.; Slootweg, P.J.; Salo, T.

    2009-01-01

    Ameloblastoma is the most common clinically significant odontogenic tumor. It is considered benign but locally invasive and associated with variable clinico-pathological behavior. Ameloblastic carcinoma is a malignant tumor having features of ameloblastoma in addition to cytologic atypia with or

  11. REFEREED ARTICLE

    African Journals Online (AJOL)

    Ogunbodede

    ameloblastoma or well differentiated squamous cell carcinoma. Although, it has an infiltrative pattern of growth, S.O.T. has become accepted as a distinct lesion rather than a variant of ameloblastoma. Treatment should be by conservative excision. Keywords: Squamous odontogenic tumour, report, Nigeria. INTRODUCTION ...

  12. An atypical adenomatoid odontogenic tumour in the mandible: a ...

    African Journals Online (AJOL)

    ameloblastoma should be well made to avoid extensive ablative surgery. However, AOT frequently resembles other odontogenic lesions such as dentigerous cysts or ameloblastoma. Immunohistochemically, AOT is characterized by positive ... radiographic examination for dental treatments. We have presented a case of a ...

  13. Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody.

    Science.gov (United States)

    Singhal, Namrata; Khanduri, Nitin; Kurup, Deepak; Gupta, Brijesh; Mitra, Pranjan; Chawla, Roshani

    2017-01-01

    Odontogenic Cysts & tumors originate through some aberration from the normal pattern of odontogenesis. Ameloblastoma is one of the most frequent intraosseous odontogenic tumors. However it is no longer appropriate to use the diagnosis of ameloblastoma without specifying the type. Varied-clinical entities of ameloblastoma differ in their biologic behaviour. Odontogenic cysts like dentigerous and radicular cysts are less aggressive in nature than odontogenic tumors. Recently, podoplanin commonly used as a lymphatic endothelial marker in cancers has recently been found to play a possible role in odontogenic tumorigenesis also. Therefore the purpose of this study was to immunohistochemically analyse the expression of podoplanin in ameloblastomas, KCOTs, dentigerous cysts, radicular cysts & dental follicles. Paraffin-embedded tissue specimens of 15 Ameloblastomas (7 follicular, 6 unicystic, 2 desmoplastic),10KCOTs, 5 dentigerous cysts, 5 radicular cysts & 5 dental follicles were immunohistochemically examined using antibody against podoplanin. All ameloblastomas displayed podoplanin expression in ameloblast-like cells of the epithelial islands while the stellate-reticulum like cells exhibited no or weak immunostaining. Expression of podoplanin in KCOTs was strongly positive in the cells of the basal and suprabasal layers & odontogenic epithelial nests. Positive immunoreaction for podoplanin was observed in the inflammatory radicular cysts and inflamed dentigerous cyst only and negative or weak expression in the lining epithelium of uninflamed dentigerous cysts and dental follicles. Our results suggest that podoplanin can be used as a potential proliferative marker to observe the aggressive behaviour of ameloblastomas and KCOTs.

  14. Ameloblastic carcinoma

    Directory of Open Access Journals (Sweden)

    Praveen B Kumar

    2009-01-01

    Full Text Available Ameloblastoma of jaws are common and locally destructive tumors originating from odontogenic apparatus. They constitute approximately 1% of all jaw tumors with 80% occurring in the mandible. Ameloblastoma exhibiting a frank malignancy is a rare entity and occurs in less than 1% of all ameloblastomas. Among the two jaws, ameloblastic carcinoma involving maxilla is extremely rare with only few cases reported so far in the literature. Here we report two cases of ameloblastic carcinoma one involving the maxilla and the other involving mandible, with an aggressive clinical course leading to extensive local destruction of the affected jaws.

  15. Ameloblastic carcinoma: A case series

    Directory of Open Access Journals (Sweden)

    Appaji Athota

    2015-01-01

    Full Text Available Ameloblastic carcinoma is a rare odontogenic tumor exhibiting not only features of ameloblastoma but also features of carcinoma in either or both primary and metastatic lesions. Clinical features of this lesion are more aggressive and rapid than those of ameloblastoma. At times, it can metastasize to the lung or regional lymph nodes. Histologically, there is a picture of both ameloblastoma and carcinoma. Treatment is aggressive and has to be designed for each individual patient. English literature is sparse for this condition, as fewer cases have been reported till date. We report a series of four cases with different treatment modalities.

  16. Keratoameloblastoma: Report of a rare variant with review of literature

    Directory of Open Access Journals (Sweden)

    Vineet Raj

    2014-01-01

    Full Text Available Ameloblastoma has intrigued clinicians as well as pathologists due to its diverse clinical behavior and histomorphologic presentations. Keratoameloblastoma is a rare histologic sub type, characterized by extensive keratin formation within ameloblastic epithelium, with only a handful number of cases described in the literature. Here, we report a case of this uncommon sub type of ameloblastoma in a young female patient presenting as an extensive lesion in mandibular ramus area. The radiological and fine needle aspiration findings suggested of a keratinizing cystic lesion and incisional biopsy showed features of ameloblastoma. Patient underwent segmental mandibulectomy and histological examination of excisional specimen revealed features of ameloblastoma with abundant keratinization leading to a diagnosis of keratoameloblastoma. The diagnostic pitfalls related with the lesion have been discussed along with a short review of the literature.

  17. Author Details

    African Journals Online (AJOL)

    : a review of the literature. Abstract · Vol 18, No 2 (2010) - Articles Hemangiomatous ameloblastoma: report of a case. Abstract · Vol 19, No 1 (2011) - Articles Clinical options for mandibular reconstruction: A review. Abstract. ISSN: 0189-1006.

  18. Spindle cell variant of ameloblastic carcinoma arising from an unicystic amelobastoma: Report of a rare case

    Directory of Open Access Journals (Sweden)

    Venkatesh V Kamath

    2012-01-01

    Full Text Available Malignant transformation of ameloblastomas arising from an odontogenic cyst or de novo is well-recognized. Malignancies in ameloblastomas may involve metastasis or a local dysplastic change in the tissue. The latter are classified as ameloblastic carcinomas. A 75-year-old male presented with a mandibular cystic swelling, with no evidence of metastasis. Dysplastic ameloblastic cells with spindle-cell transformation were seen arising from a cystic lining with features of a unicystic ameloblastoma. Immunohistochemically the lesion stained positive with cytokeratin 8,19 and alpha smooth muscle actin, but was negative for vimentin. A diagnosis of spindle-cell ameloblastic carcinoma was made. Spindle-cell ameloblastic carcinomas are rare and this is the second case arising from a unicystic ameloblastoma reported in literature. The recognition of this transformation and inclusion of this entity in the classification of ameloblastic carcinomas is stressed.

  19. melanotic neuroectodermal tumour of infancy (progonoma) treated ...

    African Journals Online (AJOL)

    2009-06-01

    Jun 1, 2009 ... tumour recurrence. Key words: Melanotic neuroectodermal tumour of infancy, melanotic progonoma, radical maxillary sur- gery. INTRODUCTION. Melanotic neuroectodermal tumour of infancy (MNTI), also known as melanotic progonoma, melano- ameloblastoma or retinal anlage tumour is a rare lo-.

  20. Keratoameloblastoma: Report of a rare variant with review of literature

    OpenAIRE

    Raj, Vineet; Chandra, Shaleen; Bedi, Ravinder Singh; Dwivedi, Ruby

    2014-01-01

    Ameloblastoma has intrigued clinicians as well as pathologists due to its diverse clinical behavior and histomorphologic presentations. Keratoameloblastoma is a rare histologic sub type, characterized by extensive keratin formation within ameloblastic epithelium, with only a handful number of cases described in the literature. Here, we report a case of this uncommon sub type of ameloblastoma in a young female patient presenting as an extensive lesion in mandibular ramus area. The radiological...

  1. Individualized Treatment for the Mandibular Segmental Defect: A Case Report

    OpenAIRE

    Zhang, Zhen; Pan, Juli; Huang, Xin; Chen, Su

    2014-01-01

    Ameloblastomas are slowly growing, locally invasive tumors with high recurrence rate and more common in the mandible, if not treated they can grow to enormous size. Radical resection is the only predictable form of treatment for ameloblastomas. However, mandibular resection can lead to dysfunctions in appearance, speech, mastication, and deglutition, which severely impair the patients’ quality of life. The reconstruction of extensive bone defects in the maxillofacial area is still challenging...

  2. Odontoameloblastoma: A rare case with unusual presentation

    Directory of Open Access Journals (Sweden)

    Supreet Jain

    2016-01-01

    Full Text Available The odontoameloblastoma (OA, also known as ameloblastic odontoma, is a very rare odontogenic tumor that is similar to ameloblastoma in its locally aggressive behavior. OA includes odontogenic ectomesenchyme in addition to odontogenic epithelium that resembles an ameloblastoma both in structure and in behavior. Its clinical presentation, however, often mimics the more innocuous odontoma, and hence, the recognition of its aggressive nature is commonly only ascertained after its histopathologic diagnosis following enucleation. This paper presents a rare case of OA with unusual clinical and radiological features.

  3. Dentinoameloblastoma with ghost cells: A rare case report with emphasis on its biological behavior

    Directory of Open Access Journals (Sweden)

    Kiran Kumar

    2013-01-01

    Full Text Available Ameloblastomas are regarded as a homogeneous group of neoplasms with locally invasive character. They generally do not show induction of dental hard tissue formation except in few cases. Biological behavior and histogenesis of these tumors is still unexplored as there is lack of relevant studies and long follow-up of these patients. So, we aimed to report this rare case of dentinoameloblastoma with unique presence of ghost cells in middle-aged female involving maxilla with emphasis on its biological behavior. We conclude that although histogenesis of this tumor is not clear but biological potential is similar to conventional ameloblastoma requiring wider excision.

  4. POLIMORFISME GEN VITAMIN D RESEPTOR SEBAGAI FAKTOR RISIKO TERJADINYA SUPERNUMBERARY TEETH

    OpenAIRE

    Asmawati; Mochammad Hatta; Bahruddin Thalib; Lenni Indriani

    2014-01-01

    Latar Belakang: Anomali gigi supernumerary teeth adalah kelainan bentuk dan jumlah gigi yang dapat menyebabkan masalah maloklusi, menganggu estetik dan deviasi ordinat gigi. Pada kondisi yang tidak erupsi dapat menyebabkan ameloblastoma dan odontogenic neoplasma. Adanya dugaan kuat faktor herediter sebagai penyebab terjadinya sehingga memerlukan penelitian genetik molekuler untuk membuktikan faktor genetik sebagai faktor risiko terjadinya supernumerary teeth,sehingga meningkatkan optimalisa...

  5. supernumerary teeth, polimorfisme gen vitamin D reseptor (VDR)

    OpenAIRE

    ASMAWATI

    2014-01-01

    Latar Belakang: Anomali gigi supernumerary teeth adalah kelainan bentuk dan jumlah gigi yang dapat menyebabkan masalah maloklusi, menganggu estetik dan deviasi ordinat gigi. Pada kondisi yang tidak erupsi dapat menyebabkan ameloblastoma dan odontogenic neoplasma. Adanya dugaan kuat faktor herediter sebagai penyebab terjadinya sehingga memerlukan penelitian genetik molekuler untuk membuktikan faktor genetik sebagai faktor risiko terjadinya supernumerary teeth,sehingga meningkatkan optimalisa...

  6. Clinicopathological guide to malignant bone tumours: A ...

    African Journals Online (AJOL)

    Clinicians correctly preliminarily diagnosed multiple myeloma, osteosarcoma, and ameloblastoma, but had inexperience with carcinomas and other types of sarcomas. Chronic osteomyelitis and metastatic lesions were mentioned frequently by radiologists as the diagnosis of some malignant bone tumours that turned out to ...

  7. AMELOBLASTIC CARCINOMA: REPORT OF A CASE FROM NIGERIA

    African Journals Online (AJOL)

    USER

    Ameloblastic carcinoma is a rare epithelial odontogenic tumour of the jaws which exhibits cytological features of ameloblastoma and carcinoma. A case of ameloblastic carcinoma in a 52 year old male is reported. Clinical / histological characteristics of this tumour and current knowledge on the classification of odontogenic ...

  8. Demographic presentation of odontogenic myxoma among patients ...

    African Journals Online (AJOL)

    They accounted for 10.6% of all odontogenic tumours, thus ranked the 2nd most attended odontogenic tumour in twelve year period. the 1st one being Ameloblastoma (72.2%). In this study odontogenic myxoma was found to occur in children below ten years old. There was female preponderance, but a lack of mandible ...

  9. Pattern of bone tumours at the National Orthopaedic Hospital ...

    African Journals Online (AJOL)

    The commonest benign lesions were ameloblastoma (9) and fibro-osseous lesions (11) while osteosarcoma was the commonest malignant lesion (8). Definitive diagnosis was based on clinical, plain film radiographic findings and biopsy findings. The benign lesions had excision/curettage done, while the malignant cases ...

  10. Author Details

    African Journals Online (AJOL)

    Adebayo, E.T.O. Vol 50, No 2 (2009) - Articles Maxillary Ameloblastoma: An Enigma for the Surgeon. Abstract. ISSN: 2229-774X. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use · Contact AJOL ...

  11. Anterior mandibular swelling – A Case Report | Praveen | Tanzania ...

    African Journals Online (AJOL)

    Predilection of lesions to occur at certain specific sites is of great aid in arriving at a logical diagnosis. However tendency of lesions to appear at particular site does not follow a rule book. Enigmatic lesions like ameloblastomas have varied presentation. Here is an unusual case report of a patient who presented to us with an ...

  12. Tumours and tumour-like conditions of the jaw seen in Zaria, Nigeria ...

    African Journals Online (AJOL)

    %) ameloblastomas; 33 (23.4%) fibrous dysplasia; 31 (22.0%) cemento-osseous dysplasia; 9 (6.4%) myxomas; 8 (5.7%) ameloblastic fibroma; and 3 (2.1%) adenomatoid odontogenic tumours; and 9 (6.4%) unclassified tumours. The benign ...

  13. Author Details - African Journals Online

    African Journals Online (AJOL)

    Udeabor, SE. Vol 20, No 1 (2015) - Articles PTCH-1 and MDM2 expression in ameloblastoma from a West African sub-population: Implication for chemotherapeutics. Abstract PDF. ISSN: 1937-8688. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL ...

  14. Author Details - African Journals Online

    African Journals Online (AJOL)

    Adisa, AO. Vol 20, No 1 (2015) - Articles PTCH-1 and MDM2 expression in ameloblastoma from a West African sub-population: Implication for chemotherapeutics. Abstract PDF · Vol 20, No 1 (2015) - Articles Malignant salivary gland tumours of the head and neck region: A single institutions review. Abstract. ISSN: 1937- ...

  15. ECAJS 2009 VOL 14 No 1 FINAL EDIT doc

    African Journals Online (AJOL)

    user

    microvascular free tissue (free flap) transfer techniques from 2005 to date was done. Results: 10 males ... following open tibial fractures, bomb blast injuries to the face, Noma, Romberg's disease, ameloblastoma. ... This is a review of the first consecutive free tissue transfers performed at Mengo hospital from. 2005 to date.

  16. Browse Title Index

    African Journals Online (AJOL)

    Items 701 - 750 of 2005 ... Vol 82, No 6 (2005):, Evaluation Of Quality Of Life Among Patients After Extirpation Of Mandibular Ameloblastoma, Abstract. ENM Simon, MAW Merkx, BM Kalyanyama, FM Shubi, PJW Stoelinga. Vol 93, No 8 (2016), Evaluation of resting sites of Culex quinquefasciatus and Anopheles gambiae s.l in ...

  17. Alpha Smooth Muscle Actin Expression in a Case of Ameloblastic Carcinoma: a Case Report

    Directory of Open Access Journals (Sweden)

    Swati Roy

    2013-02-01

    Full Text Available Background: The aim of the present article is to report a case of ameloblastic carcinoma and use a marker alpha smooth muscle actin as a tool to differentiate cases of ameloblastic carcinoma from that of ameloblastoma. Methods: Case study reporting a case of ameloblastic carcinoma (AC with expression of alpha smooth muscle actin (alpha-SMA as a marker for emergence of stromal myofibroblasts. The expression of myofibroblasts was also compared with that of ameloblastoma. Results: Difference between the two lesions in the pattern of expression of alpha smooth muscle actin was also observed. There was increase in the number of myofibroblasts in the stroma of AC while in ameloblastoma, it was comparatively less. Secondly, few areas of the carcinomatous ameloblastic island also exhibited a mild positivity towards alpha smooth muscle actin. Conclusions: Increase in number of stromal myofibroblast may be taken as a predictor for carcinomatous transformation. Further studies with greater sample size can validate the use of alpha-SMA as a marker to differentiate ameloblastic carcinoma from ameloblastoma.

  18. Squamous odontogenic tumour: report of five cases from Nigeria ...

    African Journals Online (AJOL)

    This variation may be due the limited number cases studied, but are however important additions to the few reported cases. Conclusion: Care should be taken not to misdiagnose this condition as acanthomatous ameloblastoma or well differentiated squamous cell carcinoma. Although, it has an infiltrative pattern of growth, ...

  19. Author Details

    African Journals Online (AJOL)

    Mahendra, A. Vol 20, No 2 (2014) - Articles Association Between Use of Tobacco and Age on Graying of Hair Abstract PDF · Vol 21, No 1 (2015) - Articles Basal Cell Ameloblastoma: A Rare Histological Variant of an Uncommon Tumor Abstract PDF. ISSN: 1117-6806. AJOL African Journals Online. HOW TO USE AJOL.

  20. Author Details

    African Journals Online (AJOL)

    Abstract PDF · Vol 10, No 1 (2012) - Articles Oral Amelanotic Melanoma Abstract PDF · Vol 11, No 1 (2013) - Articles Odontogenic tumours in Children and Adilescents: A Review od Forty-Eight Cases Abstract PDF · Vol 12, No 1 (2014) - Articles Cystic Ameloblastoma: A Clinico-Pathologic Review Abstract PDF. ISSN: 1597- ...

  1. Ameloblastic carcinoma: Report of a case | Arotiba | Nigerian ...

    African Journals Online (AJOL)

    Ameloblastic carcinoma is a rare epithelial odontogenic tumour of the jaws which exhibits cytological features of ameloblastoma and carcinoma. A case of ameloblastic carcinoma in a 52 year old male is reported. Clinical / histological characteristics of this tumour and current knowledge on the classification of odontogenic ...

  2. Author Details

    African Journals Online (AJOL)

    Lawal, AO. Vol 18, No 1 (2010) - Articles Trends of oral cancer in University College Hospital, Ibadan, Nigeria Abstract · Vol 18, No 2 (2010) - Articles Hemangiomatous ameloblastoma: report of a case. Abstract · Vol 22, No 1 (2014) - Articles Lymphoepitheloal-like carcinoma of the submandibular gland. Abstract.

  3. Author Details

    African Journals Online (AJOL)

    Arotiba, J. Vol 20, No 2 (2010) - Articles Biologic, anatomic and clinical considerations in the management of the classic interaosseous ameloblastoma of the jaws. Abstract. ISSN: 0189-2657. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's ...

  4. Author Details

    African Journals Online (AJOL)

    Grewal, P. Vol 21, No 1 (2015) - Articles Basal Cell Ameloblastoma: A Rare Histological Variant of an Uncommon Tumor Abstract PDF. ISSN: 1117-6806. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of ...

  5. Author Details

    African Journals Online (AJOL)

    Kharat, S. Vol 21, No 1 (2015) - Articles Basal Cell Ameloblastoma: A Rare Histological Variant of an Uncommon Tumor Abstract PDF. ISSN: 1117-6806. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of ...

  6. Author Details

    African Journals Online (AJOL)

    Omisakin, O.O. Vol 50, No 2 (2009) - Articles Maxillary Ameloblastoma: An Enigma for the Surgeon. Abstract. ISSN: 2229-774X. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use · Contact AJOL · News.

  7. Post-operative re-construction of dentoalveolar tissue and the ...

    African Journals Online (AJOL)

    Individual patient follow-up periods ranged from seven days for two patients who had cyst enucleation to two years for three cases with ameloblastoma, and two cases with squamous cell carcinoma. Conclusion: It is concluded that prosthetic rehabilitation of patients undergoing extensive surgery at the University of Nairobi ...

  8. Pattern of odontogenic tumours in Nigeria: a review of the literature ...

    African Journals Online (AJOL)

    Result: Ameloblastoma was found to be the most reported odontogenic tumour, and has been described as the most frequently occurring odontogenic tumour in Nigeria. Although malignant variants of odontogenic tumours were well recognized, they were less reported in Nigeria than in the rest of the world. Peak age of ...

  9. Odontogenic tumors: A review of 675 cases in Eastern Libya | Goteti ...

    African Journals Online (AJOL)

    The diagnosis was based on the most recent WHO (2005) classification of OTs. Results: OTs constituted 12.6% of all oral/jaw tumors and tumor-like lesions. Ameloblastoma (28.2%) was the most common type, followed by keratocystic odontogenic tumor (25.2%) and odontoma (19.9%). The male: female ratio was 1.2:1, and ...

  10. Author Details

    African Journals Online (AJOL)

    Chom, N.D. Vol 50, No 2 (2009) - Articles Maxillary Ameloblastoma: An Enigma for the Surgeon. Abstract. ISSN: 2229-774X. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use · Contact AJOL · News.

  11. Author Details

    African Journals Online (AJOL)

    Shubi, FM. Vol 9, No 2 (2002) - Articles Ameloblastoma in Tanzania: A retrospective analysis of histological records. Abstract · Vol 10, No 1 (2003) - Articles Management of most common oral HIV/AIDS manifestations of HIV/AIDS Abstract. ISSN: 0856-0625. AJOL African Journals Online. HOW TO USE AJOL.

  12. Author Details

    African Journals Online (AJOL)

    Samaila, M.A.O. Vol 50, No 2 (2009) - Articles Maxillary Ameloblastoma: An Enigma for the Surgeon. Abstract. ISSN: 2229-774X. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use · Contact AJOL ...

  13. Indications and Outcome of Mandibular Condylar and Ramus ...

    African Journals Online (AJOL)

    Background: Different surgical procedures are utilized for treatment of various diseases of the posterior mandible depending on the type of disease, site, duration and age ... 16 procedures were done for tumors in 16 (69.6%) patients, 14 (60.9%) patients had Ameloblastoma, 1 (4.3%) had central neurofibroma and one had ...

  14. Orofacial tumours and tumour-like lesions in Kano, Nigeria | Arotiba ...

    African Journals Online (AJOL)

    The most prevalent tumours were squamous cell carcinoma (46% of malignant lesions) and ameloblastoma (31% of benign lesions) the mandible (38.2%) and the maxilla (23.6%) were the most commonly affected sites. Patients usually delayed before seeking treatment and the mean duration of tumours was 30 months ...

  15. Autotransplantation of two maxillary premolars in a free vascularized fibula reconstructed mandible

    NARCIS (Netherlands)

    Mensink, G.; Karagozoglu, K.H.; Strackee, S.D.; van Teeseling, R.A.; Smeele, L.E.; Becking, A.G.

    2011-01-01

    A 10-year-old female patient with a fibula transplant in her left hemimandible due to ameloblastoma treatment was referred for combined orthodontic and surgical planning and treatment to observe and prevent expected asymmetric facial development and malocclusion, because the premolars and molars on

  16. Clinicopathological Characteristics of Odontogenic Myxoma in ...

    African Journals Online (AJOL)

    Odontogenic epithelium was rare and observed in 5 (9.5%) cases while calcific material was present in sixteen (25.4%) cases. Late presentation was a common feature and surgical excision was the treatment of choice. Conclusion: Odontogenic myxoma is un-common among Nigerians when compared with ameloblastoma ...

  17. Author Details

    African Journals Online (AJOL)

    ... Vol 38, No 3 (2004) - Articles Benign fibro-osseous lesions of the facial skeleton: an analysis of 52 cases seen at the Korle Bu Teaching Hospital Abstract PDF · Vol 38, No 3 (2004) - Articles Traumatic herniation of the buccal fat pad: a case report. Abstract PDF · Vol 45, No 1 (2011) - Articles Treatment of ameloblastoma of ...

  18. Tanzania Dental Journal - Vol 9, No 2 (2002)

    African Journals Online (AJOL)

    Ameloblastoma in Tanzania: A retrospective analysis of histological records · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. J Moshy, HJ Mosha, PGN Rugarabamu, FM Shubi, 2-4. A study to determine the prevalence of impacted third molars among patients seen in Dar es ...

  19. Download this PDF file

    African Journals Online (AJOL)

    8 F 27 Ameloblastoma Right parasymphyseal + body 2 Satisfactory 24. 9 M 14 Ameloblastic fibroma Left ... 19 F 22 Amelobastoma Right Symphyseal + body + angle + ascending ramus 4 Satisfactory 7. 20 M 23 Amelobastoma Lefi .... Keller EE, Tolman VD, Eckert S. Endosseous implant and autogcnous bone graft ...

  20. Immediate reconstruction of the mandible after resection for aggressive odontogenic tumours: a cohort study

    NARCIS (Netherlands)

    Simon, E.N.; Merkx, M.A.W.; Kalyanyama, B.M.; Shubi, F.M.; Stoelinga, P.J.W.

    2013-01-01

    The results are reported of a clinical follow-up study on 32 selected but consecutive patients with mandibular ameloblastoma. They were all treated by a segmental resection and reconstructed, using two 2.3 mm reconstruction plates and an autogenous particulate bone graft, taken from the anterior

  1. Peripheral dentinogenic ghost cell tumor

    Directory of Open Access Journals (Sweden)

    Sushant S Kamat

    2013-01-01

    Full Text Available Dentinogenic ghost cell tumors (DGCT are uncommon lesions mainly with rare peripheral types. This report presents a case of peripheral DGCT on the left side of the mandibular alveolar ridge of a heavy smoker, a 68-year-old man, with main presenting feature as a mild pain. Submandibular lymphadenopathy and radiological "saucerization" were evident. Differential diagnosis included fibroma, neurofibroma, peripheral ameloblastoma, peripheral odontogenic fibroma, and peripheral giant cell granuloma. Histologically, ameloblastoma-like epithelial elements were seen in association with grouped ghost cells. Proliferating polyhedral cells and stellate reticulum-like cells with various densities were spread over a wide range of the field. The lesion was curetted and after 2 years of follow up, it did not recur.

  2. Role of radiology in central hemangioma of jaws

    OpenAIRE

    Jindal, Sanjeev Kumar; Sheikh, Soheyl; Singla, Anshu; Puri, Nidhi

    2010-01-01

    Central hemangioma, a bone destructive lesion, is a benign tumor. Hemangiomas of jaws are rare entity and produ- ces many different radiographic images. Radiographic differential diagnosis includes osteosarcoma, fibrous dysplasia, central giant cell granuloma, ameloblastoma, odontogenic myxoma, multiple myeloma, dentigerous cyst and aneurysmal bone cyst. Since it involves proliferation of blood vessels so aspiration or biopsy of such lesions can lead to severe hemorrhage which can ...

  3. A study of biological chemistry on the nature of jaw cysts. On the maintainance of homoeostasis in jaw cyst fluid.

    Science.gov (United States)

    Suzuki, M

    1975-06-01

    Jaw cyst lining cells have an active transporting mechanism for Na+ ion and K+ion, a secreting mechanism and a selecting mechanism, and they allow permeation of electrolytes, lipids and protein into cysts. The components within the cysts have a controlling metabolism, and keep the system stable. Tumour wall cells of cystic ameloblastoma have only a passive transporting mechanism for various substances. Their nature differs from that of jaw cyst lining cells.

  4. Benign Tumors and Tumor-like Lesions of the Oral Cavity and Jaws ...

    African Journals Online (AJOL)

    2016-06-15

    Jun 15, 2016 ... nonodontogenic benign tumors and tumor-like lesions. Pathology. Male Female Male:female ratio. Total (%). Odontogenic. KCOT. 35. 16. 2.2:1. 51 (42.2). Compound odontoma. 11. 12. 1:1.1. 23 (19). Complex odontoma. 10. 7. 1.4:1. 17 (14.1). Ameloblastoma. 4. 10. 1:2.5. 14 (11.6). Odontogenic myxoma. 2.

  5. Decompression Device Using a Stainless Steel Tube and Wire for Treatment of Odontogenic Cystic Lesions: A Technical Report.

    Science.gov (United States)

    Jung, Eun-Joo; Baek, Jin-A; Leem, Dae-Ho

    2014-11-01

    Decompression is considered an effective treatment for odontogenic cystic lesions in the jaw. A variety of decompression devices are successfully used for the treatment of keratocystic odontogenic tumors, radicular cysts, dentigerous cysts, and ameloblastoma. The purpose of these devices is to keep an opening between the cystic lesion and the oral environment during treatment. The aim of this report is to describe an effective decompression tube using a stainless steel tube and wire for treatment of jaw cystic lesions.

  6. Odontogenic tumors: analysis of 706 cases.

    Science.gov (United States)

    Regezi, J A; Kerr, D A; Courtney, R M

    1978-10-01

    From a total of 54,534 oral biopsy specimens, 706 (1.3%) odontogenic tumors were retrieved and reviewed. Odontomas comprised more than 65% of the odontogenic tumors, ameloblastomas about 10%, and the remaining six categories of odontogenic tumors accounted for approximately 25% of the lesions. The distribution by age, sex, and location of these tumors generally supported the data from other previously reported cases. A possible variant of the calcifying epithelial odontogenic tumor was described, and instances of two granular cell ameloblastic fibromas were reported. The myxomas as a group were characterized histologically more by residual bony trabeculae than by the presence of odontogenic rests. Because the clinical, histological, and behavioral features of the ameloblastic fibroma and ameloblastic fibro-odontoma were similar, these lesions were considered to be essentially the same. From limited follow-up information, the ameloblastoma was the only lesion that recurred. With the exception of one ameloblastoma found in the lung, no malignant odontogenic tumors were encountered.

  7. ESEM Detection of Foreign Metallic Particles inside Ameloblastomatous Cells.

    Science.gov (United States)

    Roncati, Luca; Gatti, Antonietta Morena; Pusiol, Teresa; Barbolini, Giuseppe; Maiorana, Antonio; Montanari, Stefano

    2015-01-01

    Ameloblastoma is a borderline tumor of odontogenic origin, with a high recurrence rate and possible local aggressiveness. The etiopathogenetic factors involved in its occurrence are not still defined and our study has been precisely aimed to search for novel factors associated with its development. Sections cut from paraffin blocks, containing the representative specimens of 18 different ameloblastomas, collected in a 15-year period (1999-2014), have been observed by an environmental scanning electron microscope, in order to search micro- and nano-sized particles and to identify their composition. In all the neoplastic cases, micro- and nano-sized metallic debris, differing in size and composition, have been detected inside the ameloblastomatous cells. On the contrary, the total absence of metallic particles in the healthy control cases has been emerged. Our results reveal a relationship between ameloblastoma and metallic particulate. The cigarette smoke and the routine dental practice appear the most probable source for the presence of these biopersistant inorganic particles inside the neoplastic cells.

  8. FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes

    Science.gov (United States)

    Kuga, Takahisa; Sasaki, Mitsuho; Mikami, Toshinari; Miake, Yasuo; Adachi, Jun; Shimizu, Maiko; Saito, Youhei; Koura, Minako; Takeda, Yasunori; Matsuda, Junichiro; Tomonaga, Takeshi; Nakayama, Yuji

    2016-01-01

    FAM83H is essential for the formation of dental enamel because a mutation in the FAM83H gene causes amelogenesis imperfecta (AI). We previously reported that the overexpression of FAM83H often occurs and disorganizes the keratin cytoskeleton in colorectal cancer cells. We herein show that FAM83H regulates the organization of the keratin cytoskeleton and maintains the formation of desmosomes in ameloblastoma cells. FAM83H is expressed and localized on keratin filaments in human ameloblastoma cell lines and in mouse ameloblasts and epidermal germinative cells in vivo. FAM83H shows preferential localization to keratin filaments around the nucleus that often extend to cell-cell junctions. Alterations in the function of FAM83H by its overexpression, knockdown, or an AI-causing truncated mutant prevent the proper organization of the keratin cytoskeleton in ameloblastoma cells. Furthermore, the AI-causing mutant prevents desmosomal proteins from being localized to cell-cell junctions. The effects of the AI-causing mutant depend on its binding to and possible inhibition of casein kinase I (CK-1). The suppression of CK-1 by its inhibitor, D4476, disorganizes the keratin cytoskeleton. Our results suggest that AI caused by the FAM83H mutation is mediated by the disorganization of the keratin cytoskeleton and subsequent disruption of desmosomes in ameloblasts. PMID:27222304

  9. Ameloblastomatous calcifying odontogenic cyst: A rare histologic variant

    Directory of Open Access Journals (Sweden)

    Basavaraj N Kallalli

    2015-01-01

    Full Text Available Ameloblastoma is a well-known odontogenic tumor that can be associated with calcifying odontogenic cysts (COCs, but only a few reports give its clinical and radiographic features. Calcifying odontogenic cyst was first categorized as a distinct entity by Gorlin et al., and has been named after him since then. Calcifying odontogenic cyst is an uncommon developmental odontogenic lesion that demonstrates histopathologic diversity. It is well known that this lesion can occur in association with odontogenic tumors such as complex odontoma and ameloblastoma. The term COC was not included by the World Health Organization (WHO in its report of 2005 and is called calcifying cystic odontogenic tumor (CCOT. Histopathologic examination of ameloblastomatous CCOT reveals ameloblastic islands containing ghost cells. Although association of ameloblastoma with this lesion is important, only a few cases have been reported in literature. The present case report is of ameloblastomatous calcifying cystic odontogenic tumor, a rare histologic variant, in a 20-year-old male patient in the left mandibular posterior region.

  10. CD56 Expression in Odontogenic Cysts and Tumors

    Directory of Open Access Journals (Sweden)

    Zohreh Jaafari-Ashkavandi

    2014-12-01

    Full Text Available Background and aims. Odontogenic cysts and tumors have a wide spectrum of clinical characteristics that lead to the different management strategies. Since definite diagnosis is difficult in some cases, it has been suggested that CD56 may be a candidate marker for definitive diagnosis of some odontogenic tumors. The present study was designed to examine CD56 expression in lesions with histopathological similarities. Materials and methods. In this cross-sectional, analytical study the subjects were 22 ameloblastomas, 13 dentigerous cysts, 10 keratocystic odontogenic tumors (KCOT, 4 adenomatoid odontogenic tumors (AOT, 3 orthokeratinized odontogenic cysts, 3 calcifying odontogenic cysts (COC and one glandular odontogenic cyst (GOC. All the samples were examined for CD56 immunoreactivity. Data were analyzed using chi-square test. Results. Twenty cases (91% of ameloblastomas, 3 (75% AOT, 4 (40% KCOT and one case of GOC were positive for CD56. None of the dentigerous cysts, COC and orthokeratinized odontogenic cysts was CD56-positive. There was a significant difference in the CD56 expression between ameloblastoma and dentigerous cyst, as well as COC. Also, KCOT showed significantly higher expression than orthokeratinized odontogenic cyst. Conclusion. In this study CD56 expression was limited to the odontogenic tumors and more aggressive cystic lesions. This marker can be a useful aid for distinguishing cysts and tumors from similar lesions.

  11. Individualized treatment for the mandibular segmental defect: a case report.

    Science.gov (United States)

    Zhang, Zhen; Pan, Juli; Huang, Xin; Chen, Su

    2015-04-01

    Ameloblastomas are slowly growing, locally invasive tumors with high recurrence rate and more common in the mandible, if not treated they can grow to enormous size. Radical resection is the only predictable form of treatment for ameloblastomas. However, mandibular resection can lead to dysfunctions in appearance, speech, mastication, and deglutition, which severely impair the patients' quality of life. The reconstruction of extensive bone defects in the maxillofacial area is still challenging. To meet the demands of functional reconstruction, minimizing the negative influence of mandibular malformation, and disability on patients, the individualized systematic treatment plans highlight denture prosthodontics and require much consideration of multidisciplinary cooperation, with such related fields as maxillofacial surgery, oral implantology, prosthodontics, and radiology taken into account. In this report, we will present a case of reconstructing the mandibular segmental defect after the resection of a rarely giant ameloblastoma. In the case, we took the restoration of the missing teeth and the rehabilitation of the masticatory function as well as restoring bony continuities and facial appearance into consideration, communicated well with prosthodontists and implantologists before surgery, making the individualized systematic treatment plan more effective and efficient.

  12. Neoplasms associated with dentigerous cyst: An insight into pathogenesis and clinicopathologic features

    Directory of Open Access Journals (Sweden)

    Jitendra V Kalburge

    2015-01-01

    Full Text Available Odontogenic cysts may occur in association with odontogenic tumors. Because neoplastic and hamartomatous aberrations can occur at any stage of odontogenesis, combined features of odontogenic tumors with epithelial and mesenchymal components may arise within odontogenic cysts. One of the most common of these is dentigerous cyst (DC which has neoplastic potential and shows associated pathologies such as ameloblastoma, squamous cell carcinoma, mucoepidermoid carcinoma (MEC, adenomatoid odontogenic tumor (AOT, and odontoma. The authors report four cases of DC and associated lesions exhibiting AOT in two cases while one case each of complex odontome and MEC. Emphasis is placed on pathogenesis and clinicopathologic features of these lesions.

  13. Odontoameloblastoma: Report of two cases

    Directory of Open Access Journals (Sweden)

    Mosca Rodrigo

    2009-01-01

    Full Text Available Odontoameloblastoma (OA is a very rare mixed odontogenic neoplasm, characterized by the simultaneous occurrence of an ameloblastoma and a compound or complex odontoma in the same tumor mass. To date, less than 50 cases of OA and/or ameloblastic odontoma have been reported in the English dental literature. This neoplasm was called ameloblastic odontoma. The term OA was included in the 1971 WHO classification. In this study, we present two cases of OA, which we hope will contribute to the awareness and knowledge of surgeons regarding the existence of this odontogenic tumor so that patients having it may be treated and followed-up properly.

  14. Odontoma associated with calcifying cystic odontogenic tumor in deciduous dentition: case report.

    Science.gov (United States)

    Poleti, Marcelo Lupion; Duarte, Bruno Gomes; Lara, Vanessa Soares; Freitas-Faria, Patrícia; Rubira-Bullen, Izabel Regina Fischer; Gonçales, Eduardo Sanches

    2013-03-01

    Initially described by Gorlin et al. in 1962, the calcifying cystic odontogenic tumor (CCOT) may be associated with unerupted teeth, ameloblastomas, adenomatoid odontogenic tumors, and, in many cases, with odontomas. It is rare in patients in the first decade of life, particularly involving deciduous teeth. Surgery is the treatment of choice, with low recurrence rates. We present a clinical case of CCOT associated with odontoma and a missing deciduous tooth in a 3-year-old female patient. The lesion was removed under general anesthesia. The patient has been followed up for 1 year, and no recurrence was found. This appears to be the first report in such a young age.

  15. Histopathological insight of complex odontoma associated with a dentigerous cyst.

    Science.gov (United States)

    Astekar, Madhusudan; Manjunatha, Bhari Sharanesha; Kaur, Prabhpreet; Singh, Jappreet

    2014-01-31

    Odontomas and dentigerous cysts are common findings for practicing dental professionals. However, simultaneous occurrence of pathologies like odontoma and dentigerous cyst are uncommon and their diagnosis based on the radiographic appearance alone is a challenge to overcome. They together are a potential for complications like attaining large size, root resorption, destruction of the jaw bones and sometimes neoplastic changes like ameloblastoma. This paper presents a case of complex odontoma associated with dentigerous cyst in relation to a retained deciduous tooth in the maxillary anterior region and confirming its diagnosis histopathologically.

  16. CK13 in craniopharyngioma versus related odontogenic neoplasms and human enamel organ.

    Science.gov (United States)

    el-Sissy, N A; Rashad, N A

    1999-05-01

    The monoclonal antibody NCL-CK13 was studied in specimens of craniopharyngioma, ameloblastoma and calcifying odontogenic cyst neoplasms and the mandible and maxillae of normal human fetuses. There was a decrease in NCL-CK13 as the dental lamina developed, with a complete loss in the enamel organ. The neoplastic epithelia of the neoplasms revealed a clear phenotypic and immunohistochemical reactive relationship to the stratified embroyonic mucosa, away from the enamel organ. This suggests that these neoplasms might have their histogenesis from early stage epithelium, the oral part of the dental lamina or its remnants.

  17. Papilliferous Keratoameloblastoma: An Extremely Rare Case Report

    Directory of Open Access Journals (Sweden)

    Neeta Mohanty

    2013-01-01

    Full Text Available Odontogenic tumors develop in the jaw bones from the odontogenic tissue-oral epithelium in tooth germ, enamel organ, dental papilla, reduced enamel epithelium, remnants of Hertwig’s root sheath or dental lamina, and so forth. Hence, a bewildering variety of tumors are encountered in the maxilla and mandible. Ameloblastoma is the second most common odontogenic neoplasm after odontomes, and it has numerous clinical and histologic variants. We report a very rare histologic variant: the papilliferous keratoameloblastoma which is the fifth reported case in the English literature.

  18. Carcinoma ameloblástico de mandíbula Carcinoma ameloblástico de la mandíbula Ameloblastic carcinoma of the mandible

    Directory of Open Access Journals (Sweden)

    Cláudia Roberta Leite Vieira de Figueiredo

    2012-06-01

    Full Text Available O termo carcinoma ameloblástico é usado para descrever ameloblastomas com características histológicas de malignidade. Lesões desse tipo podem ocorrer em uma extensa faixa etária, sendo mais comum na quarta década de vida. Não há predileção aparente por sexo, a área mais comumente afetada é a porção posterior da mandíbula, envolvendo em menor proporção a maxila. Por se tratar de um tumor com poucos casos descritos na literatura, o objetivo deste trabalho foi relatar um caso de carcinoma ameloblástico em paciente do sexo feminino, 38 anos, com queixa de dor e assimetria facial. Ao exame clínico intrabucal observou-se um aumento de volume em região posterior de mandíbula, do lado direito. A tomografia computadorizada revelou adelgaçamento das corticais com presença de área hipodensa homogênea. Foi realizada biópsia incisional cujo diagnóstico histológico inicial foi de ameloblastoma, sem características citológicas de malignidade. Baseando-se no exame histopatológico, optou-se pelo tratamento cirúrgico da lesão através da ressecção parcial da mandíbula. A peça cirúrgica foi encaminhada ao laboratório obtendo-se novo diagnóstico de carcinoma ameloblástico. Provavelmente, a diferença do diagnóstico histológico final para o inicial pode estar relacionada ao fato de o carcinoma ameloblástico apresentar áreas focais semelhantes ao ameloblastoma. Após 6 meses da cirurgia, a paciente apresentou metástase tumoral no pulmão e encontra-se sob supervisão médica.El término carcinoma ameloblástico es utilizado para describir ameloblastomas con características histológicas de malignidad. Este tipo de lesión puede ocurrir en cualquier edad, pero se puede observar con mayor frecuencia en la cuarta década de vida. No existe aparentemente un sexo predominante. El área más comúnmente afectada es la porción posterior de la mandíbula y afecta en menor proporción el maxilar. Por ser un tumor con pocos

  19. Jaw lesions associated with impacted tooth: A radiographic diagnostic guide

    Energy Technology Data Exchange (ETDEWEB)

    Motazavi, Hamed; Bharvand, Maryam [Dept. of Oral Medicine, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran (Iran, Islamic Republic of)

    2016-09-15

    This review article aimed to introduce a category of jaw lesions associated with impacted tooth. General search engines and specialized databases such as Google Scholar, PubMed, PubMed Central, MedLine Plus, Science Direct, Scopus, and well-recognized textbooks were used to find relevant studies using keywords such as 'jaw lesion', 'jaw disease', 'impacted tooth', and 'unerupted tooth'. More than 250 articles were found, of which approximately 80 were broadly relevant to the topic. We ultimately included 47 articles that were closely related to the topic of interest. When the relevant data were compiled, the following 10 lesions were identified as having a relationship with impacted tooth: dentigerous cysts, calcifying odontogenic cysts, unicystic (mural) ameloblastomas, ameloblastomas, ameloblastic fibromas, adenomatoid odontogenic tumors, keratocystic odontogenic tumors, calcifying epithelial odontogenic tumors, ameloblastic fibro-odontomas, and odontomas. When clinicians encounter a lesion associated with an impacted tooth, they should first consider these entities in the differential diagnosis. This will help dental practitioners make more accurate diagnoses and develop better treatment plans based on patients' radiographs.

  20. Expression of the transcription factor PITX2 in ameloblastic carcinoma.

    Science.gov (United States)

    García-Muñoz, Alejandro; Rodríguez, Mario A; Licéaga-Escalera, Carlos; Licéaga-Reyes, Rodrigo; Carreón-Burciaga, Ramón Gil; González-González, Rogelio; Bologna-Molina, Ronell

    2015-06-01

    Ameloblastic carcinoma is a rare odontogenic tumour that combines the histological features of ameloblastoma with cytological atypia. Until 2005, the incidence of ameloblastic carcinoma was unknown, and since then, fewer than 60 cases have been reported. These tumours may originate from pre-existing tumours or cysts, or they arise de novo from the activation or transformation of embryological cells. PITX2 is a transcription factor that is a product and regulator of the WNT cell signalling pathway, which has been involved in development of several tumours. To analyse whether PITX2 could be involved in the biological behaviour of ameloblastic carcinoma, we analysed the expression of this transcription factor in a sample of this tumour and nine benign ameloblastomas to compare. The results of Western blotting and RT-PCR analyses were positive, and considering the hundreds of genes that PITX2 regulates, we believe that its expression could be intimately linked to the behaviour of ameloblastic carcinoma and possibly other odontogenic lesions. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. A CLINICOPATHOLOGICAL STUDY OF JAW LESIONS AT A TERTIARY CARE CENTRE

    Directory of Open Access Journals (Sweden)

    Naval Kishore Bajaj

    2016-08-01

    Full Text Available BACKGROUND Jaw bones are exceptional developmentally in having embryonal neuroectodermal cells on one hand and tooth germs on other. They cause destruction of the jaw bones and pose diagnostic challenge. MATERIAL AND METHODS Patients attending Oral and Maxillofacial Surgery Department at Osmania General Hospital during the period January 2013- May 2016 presenting with complaints of gradually progressive of jaw swelling, toothache and with radiological evaluation showing osteolytic, sclerotic, and cystic change were subjected to surgical excision. Representative tissue samples were processed routinely and stained by haematoxylin and eosin. RESULTS A total of 55 cases of lesions involving jaw bones were studied out of which 21 cases occurred in females and 34 cases in males. 47 lesions were encountered in mandible and 7 lesions in maxilla. A single case of firm-to-hard submandibular swelling was also included in the study. The lesions were categorised into cysts, odontogenic tumours, reactive bone lesions, giant cell lesions, and primary bone tumours. Ameloblastoma was the most common odontogenic tumour type, 15/55; one ameloblastoma case was recurrent followed by radicular cyst 7/55 and dentigerous cyst accounting for six cases and variety of other lesions. CONCLUSION A whole gamut of lesions occurred in the jaws presented with a considerable overlap in clinical, histological, and radiological features. The present study revealed mostly cystic and benign neoplastic lesions.

  2. Odontogenic tumors: analysis of 127 cases Tumores odontogênicos: análise de 127 casos

    Directory of Open Access Journals (Sweden)

    Jean Nunes SANTOS

    2001-12-01

    Full Text Available One hundred and twenty-seven cases of histologically confirmed odontogenic tumors were retrieved from a total of 5,289 oral and maxillary lesions diagnosed at the Division of Oral Pathology, Federal University of Rio Grande do Norte, during a period of 30 years (l970-l999. The most common histological diagnosis was odontoma (50.40%, followed by ameloblastoma (30.70%. The prevalence of odontogenic tumors was greater in females and the peak incidence occurred in the second and third decades of life. The main anatomical location was the mandible, and no malignant tumors were found.De uma série de 5.289 casos de lesões orais e dos maxilares diagnosticadas no Laboratório de Patologia Oral da Faculdade de Odontologia da Universidade Federal do Rio Grande do Norte no período de 30 anos (1970-1999, foram analisados 127 casos de tumores odontogênicos confirmados histologicamente. A lesão mais freqüente foi o odontoma (50,40% seguida pelo ameloblastoma (30,70%. A prevalência de tumores odontogênicos foi maior nas mulheres e o pico de incidência ocorreu na segunda e terceira décadas de vida. A localização anatômica mais comum foi a mandíbula e não foram encontrados casos de tumores malignos.

  3. Evaluation of Amelotin Expression in Benign Odontogenic Tumors

    Directory of Open Access Journals (Sweden)

    Daiana Paula Stolf

    2013-10-01

    Full Text Available Objective: Amelotin (AMTN is highly and selectively expressed by odontogenic epithelium-derived ameloblasts throughout the maturation stage of enamel formation. The protein is secreted and concentrated at the basal lamina interface between ameloblasts and the mineralized enamel matrix. Odontogenic tumors (OT are characterized by morphological resemblance to the developing tooth germ. OT vary from slowly expanding, encapsulated tumors to locally aggressive and destructive lesions. The purpose of this study was to determine the expression profile of AMTN in benign odontogenic tumors and to correlate it with specific features of the lesions. Methods: Immunohistochemical staining for AMTN was performed on human ameloblastoma, ameloblastic fibroma (AF, ameloblastic fibro-odontoma (AFO, odontoma, adenomatoid odontogenic tumor (AOT and calcifying cystic odontogenic tumor (CCOT. Results: Generally, ameloblastoma and AF did not stain for AMTN. A strong signal was detected in ameloblast-like layers of AFO and odontoma. Epithelial cells in AOT did not stain for AMTN, while calcifying areas of extracellular eosinophilic matrix were intensely stained. Interestingly, ghost cells present in odontomas and CCOT revealed variable staining, again in association with calcification foci. Conclusions: Amelotin expression was consistently detected in tumors presenting differentiated ameloblasts and obvious matrix deposition. Additionally, the presence of the protein in the eosinophilic matrix and small mineralized foci of AOT and calcification areas of ghost cells may suggest a role for AMTN in the control of mineralization events. [J Interdiscipl Histopathol 2013; 1(5.000: 236-245

  4. Bleomycin/interleukin-12 electrochemogenetherapy for treating naturally occurring spontaneous neoplasms in dogs.

    Science.gov (United States)

    Reed, S D; Fulmer, A; Buckholz, J; Zhang, B; Cutrera, J; Shiomitsu, K; Li, S

    2010-08-01

    On the basis of superior outcomes from electrochemogenetherapy (ECGT) compared with electrochemotherapy in mice, we determined the efficacy of ECGT applied to spontaneous canine neoplasms. Intralesional bleomycin (BLM) and feline interleukin-12 DNA injection combined with translesional electroporation resulted in complete cure of two recurrent World Health Organization stage T(2b)N(0)M(0) oral squamous cell carcinomas (SCCs) and one T(2)N(0)M(0) acanthomatous ameloblastoma. Three remaining dogs, which had no other treatment options, had partial responses to ECGT; one had mandibular T(3b)N(2b)M(1) melanoma with pulmonary and lymph node metastases; one had cubital T(3)N(0)M(1) histiocytic sarcoma with spleen metastases; and one had soft palate T(3)N(0)M(0) fibrosarcoma. The melanoma dog had decrease in the size of the primary tumor before recrudescence and euthanasia. The histiocytic sarcoma dog had resolution of the primary tumor, but was euthanized because of metastases 4 months after the only treatment. The dog with T(3)N(0)M(0) fibrosarcoma had tumor regression with recrudescence. Treatment was associated with minimal side effects and was easy to perform, was associated with repair of bone lysis in cured dogs, improved quality of life for dogs with partial responses and extended overall survival time. ECGT seems to be a safe and resulted in complete responses in SCC and acanthomatous ameloblastoma.

  5. Immunohistochemical Analysis of P63 Expression in Odontogenic Lesions

    Directory of Open Access Journals (Sweden)

    Saede Atarbashi Moghadam

    2013-01-01

    Full Text Available P63 may have a role in tumorigenesis and cytodifferentiation of odontogenic lesions. We investigated the immunohistochemical expression of P63 in a total of 30 cases of odontogenic cysts and tumors. The percentage of positive cells was calculated in the lining of odontogenic cysts and islands of ameloblastoma. P63 expression was evident in all types of odontogenic lesions. P63 was expressed throughout the lining epithelium of odontogenic keratocyst except surface parakeratinized layer. In addition, calcifying odontogenic cyst showed P63 expression in all layers. In almost all radicular and dentigerous cysts, the basal and parabasal layers were immunoreactive. Peripheral cells of ameloblastoma expressed P63; however, stellate reticulum had weaker immunostaining. No significant difference in P63 expression was observed between studied lesions (. Expression of P63 in odontogenic lesions suggests that this protein is important in differentiation and proliferation of odontogenic epithelial cells. However, it seems that it could not be a useful marker to differentiate between aggressive and nonaggressive lesions. P63 also represents a progenitor or basal cell marker, and it is not expressed in mature differentiated cells.

  6. ارزیابی تراکم عروق خونی در کیست فولیکولار، تومورادنتوژنیک کراتوسیست و آملوبلاستوما

    Directory of Open Access Journals (Sweden)

    صفورا سیفی

    2011-03-01

    Full Text Available Introduction: Ameloblastoma is a benign epithelial odontogenic tumor that exhibits a more aggressive behavior than follicular cyst and keratocystic odontogenic tumor. The purpose of the current study was detection and evaluation of microvessel density (MVD effect on growth and clinical behavior of above odontogenic lesions with immunohistochemistry. Materials & Methods: In this descriptive-analytic cross-sectional study, 45 paraffin blocks of mentioned lesions were selected and stained immunohistochemically with CD34. The mean MVD of each lesionin three microscopic fields was calculated separately and was evaluated in the two areas, adjacent and far from odontogenic epithelium and then were compared with one another. Mann-Whiteny and Kruskal Wallis tests were used for the statistical analysis. Results: Mean microvessl density was 40.8±15.9, 25.4±5.3, and 9.4±3.52 in ameloblastoma, keratocystic odontogenic tumor , and follicular cyst, respectively.Mean microvessl density difference between the above mentioned lesions was statistically significant (P

  7. Jaw lesions associated with impacted tooth: A radiographic diagnostic guide

    International Nuclear Information System (INIS)

    Motazavi, Hamed; Bharvand, Maryam

    2016-01-01

    This review article aimed to introduce a category of jaw lesions associated with impacted tooth. General search engines and specialized databases such as Google Scholar, PubMed, PubMed Central, MedLine Plus, Science Direct, Scopus, and well-recognized textbooks were used to find relevant studies using keywords such as 'jaw lesion', 'jaw disease', 'impacted tooth', and 'unerupted tooth'. More than 250 articles were found, of which approximately 80 were broadly relevant to the topic. We ultimately included 47 articles that were closely related to the topic of interest. When the relevant data were compiled, the following 10 lesions were identified as having a relationship with impacted tooth: dentigerous cysts, calcifying odontogenic cysts, unicystic (mural) ameloblastomas, ameloblastomas, ameloblastic fibromas, adenomatoid odontogenic tumors, keratocystic odontogenic tumors, calcifying epithelial odontogenic tumors, ameloblastic fibro-odontomas, and odontomas. When clinicians encounter a lesion associated with an impacted tooth, they should first consider these entities in the differential diagnosis. This will help dental practitioners make more accurate diagnoses and develop better treatment plans based on patients' radiographs

  8. [Expression of HOX C13 in odontogenic tumors].

    Science.gov (United States)

    Hong, Yan-song; Wang, Jie; Liu, Jie; Zhang, Bo; Hou, Lin; Zhong, Ming

    2007-12-01

    To study the expression of HOXC13 mRNA in odontogenic tumors. HOXC13 mRNA was detected in 47 cases of ameloblastoma (AB), 3 cases of calcifying cystic odontogenic tumor (CCOT), 3 cases of ameloblastic fibroma (AF), 10 cases of keratocystic odontogenic tumor (KCOT) and 2 cases of calcifying epithelial odontogenic tumor (CEOT) by in situ hybridization, and 7 cases of normal oral mucosa were selected as control. SPSS10.0 software package was used for chi(2) test. HOXC13 mRNA was positively expressed in all odontogenic epithelium except AF. The positive ratios were 97.9% in AB, 100% in CCOT, 100% in CEOT, 70.0% in KCOT epithelium and 42.9% in the normal oral mucosa. There was significant difference among AB, KCOT and normal mucosa (chi(2)=21.665, PHOXC13. The expression of HOXC13 mRNA in the odontogenic lesions has heterogeneity.

  9. Haemophilic pseudotumour in two parts of the maxilla: case report

    Science.gov (United States)

    Kwon, Ah-Young; Huh, Kyung-Hoe; Yi, Won-Jin; Symkhampha, Khanthaly; Lee, Sam-Sun; Choi, Soon-Chul

    2016-01-01

    Haemophilic pseudotumour is a rare disease that occurs most often in femur, tibia, ilium or pelvic bone of a patient with haemophilia. Thus far, there have been only 31 reported cases in jaw bones and paranasal sinuses. Among them, the mandible is a more common site than the maxilla or paranasal sinuses. Here, we report a case of haemophilic pseudotumour in two parts of the maxilla. Contrast-enhanced CT showed an expansive and thinly corticated lesion with fluid attenuation at the left anterior maxilla which seemed like a post-operative maxillary cyst, ameloblastoma or odontogenic cyst. In addition, the thickened left palatal process of the maxilla seemed like fibrous dysplasia or intraosseous vascular malformation. Since haemophilic pseudotumour is not pathognomonic in radiological findings, when a patient who suffered from haemophilia or had taken anticoagulating agents has jaw lesion, haemophilic pseudotumour should be included in a differential diagnosis. PMID:27359071

  10. Melanotic neuroectodermal tumor of infancy

    International Nuclear Information System (INIS)

    Atkinson, G.O. Jr.; Davis, P.C.; Patrick, L.E.; Winn, K.J.; Ball, T.I.; Wyly, J.B.

    1989-01-01

    Melanotic neuroectodermal tumor of infancy is an uncommon neoplasm occurring primarily in the child one year or less in age. Difficulty in deciding the cellular origin of this tumor has led to numerous names, including congenital melanocarcinoma, melanotic epithelial odontoma, melanotic ameloblastoma, and retinal anlage tumor, to list a few. Electron microscopy and histochemical studies, however, have now established the neural crest the most likely origin. The most frequent site of occurrence is the maxilla followed by the skull, the brain and the mandible. The genital organs are the most frequent extracranial site. Within the skull, there is a predilection for the anterior fontanel. The following is a case report of a young child with melanotic neuroectodermal tumor of infancy arising at the anterior fontanel. Included is a discussion of magnetic resonance (MR) findings, which to our knowledge, have not been previously reported in this tumor. (orig.)

  11. Melanotic neuroectodermal tumor of infancy. MR findings and a review of the literature

    Energy Technology Data Exchange (ETDEWEB)

    Atkinson, G.O. Jr.; Davis, P.C.; Patrick, L.E.; Winn, K.J.; Ball, T.I.; Wyly, J.B. (Emory Univ., Atlanta, GA (USA). Dept. of Radiology)

    1989-11-01

    Melanotic neuroectodermal tumor of infancy is an uncommon neoplasm occurring primarily in the child one year or less in age. Difficulty in deciding the cellular origin of this tumor has led to numerous names, including congenital melanocarcinoma, melanotic epithelial odontoma, melanotic ameloblastoma, and retinal anlage tumor, to list a few. Electron microscopy and histochemical studies, however, have now established the neural crest the most likely origin. The most frequent site of occurrence is the maxilla followed by the skull, the brain and the mandible. The genital organs are the most frequent extracranial site. Within the skull, there is a predilection for the anterior fontanel. The following is a case report of a young child with melanotic neuroectodermal tumor of infancy arising at the anterior fontanel. Included is a discussion of magnetic resonance (MR) findings, which to our knowledge, have not been previously reported in this tumor. (orig.).

  12. Clonal nature of odontogenic tumours.

    Science.gov (United States)

    Gomes, Carolina Cavaliéri; Oliveira, Carla da Silveira; Castro, Wagner Henriques; de Lacerda, Júlio César Tanos; Gomez, Ricardo Santiago

    2009-04-01

    Although clonal origin is an essential step in the comprehension of neoplasias, there have been no studies to examine whether odontogenic tumours are derived from a single somatic progenitor cell. The purpose of this study was to investigate the clonal origin of odontogenic tumours. Fresh samples of seven ameloblastomas, two odontogenic mixomas, two adenomatoid odontogenic tumour, one calcifying odontogenic cyst, one calcifying epithelial odontogenic tumour (CEOT) and six odontogenic keratocyst (OKC) of female patients were included in this study. After DNA extraction, the HUMARA gene polymorphism assay was performed. Most of the informative odontogenic lesions studied (12 out of 16) showed a monoclonal pattern. Among the polyclonal cases, two were OKC, one CEOT and one odontogenic mixoma. Our results suggest that most odontogenic tumours are monoclonal.

  13. [Calcifying odontogenic cyst. A clinical case].

    Science.gov (United States)

    Colella, G; Lanza, A; Tartaro, G P

    1995-12-01

    The calcifying odontogenic cyst (COC) is a rare lesion that involves the jaws which, at times, may evolve into a solid, benign, tumor-like mass. There is no significant sex predilection, but it is probably more common in later life. The diagnosis is base only on histologic examination that represents, in the best of cases, the only way to differentiate the COC from other pathologies. Such lesions include: odontogenic cyst, residual cyst, globulomaxillary cyst, lateral periodontal cyst, ameloblastoma (multicystic and unicystic), odontogenic mixoma, and still with calcifying odontogenic epithelial tumor (Pindborg tumor) and adenomatoid odontogenic tumor. The authors report a case of COC, starting point for a review of the clinical, radiographic and histomorphologic features of the lesion.

  14. Keratocystic odontogenic tumor: A case report and review of literature

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    Keerthi K Nair

    2015-01-01

    Full Text Available Keratocystic odontogenic tumor (KCOT has been identified as a "tumor" after observation of its biological behavior and genetic abnormalities consistent with neoplastic progression. In 2005, the World Health Organization (WHO working group considered odontogenic keratocyst (OKC to be a tumor and recommended the term KCOT, distinguishing the lesion from the orthokeratinizing variant, which is now considered an OKC or orthokeratinized odontogenic cyst. Very rarely, KCOTs can transform into more aggressive lesions such as ameloblastoma and primary intraosseous carcinoma (PIOSCC. In this paper, we present a case of KCOT involving the angle and ramus of the mandible, with histopathologic evidence of ameloblastomatous changes. We also discuss about the evolution of this lesion from a cyst to a tumor along with the latest updates of the entity.

  15. Translational cancer vaccine: from mouse to human to cat

    Science.gov (United States)

    Levenson, Richard

    2015-03-01

    Acanthomatous ameloblastoma is a locally invasive tumor arising in the gingiva that can progress rapidly, invade and destroy bone. If the lesion involves the upper jaw, surgical excision may not be possible and while local control is imperative, other therapies have not been fully evaluated. The primary author's personal cat, Gabriella, developed this tumor, with gingival masses around teeth in the upper jaw and evidence of widespread bony destruction of the hard palate. Because of his involvement with Immunophotonics Inc. as an advisor, the author was aware of an in situ autologous cancer vaccine (inCVAX) that is currently under development by the company. One session was performed in a veterinary clinic in Arkansas, and two follow-up sessions at the small animal hospital at the UC Davis veterinary school. No other therapy was provided. As of this writing, 3+ years after first treatment and 3 years, 4 months after presentation, Gabriella is well, with no evidence of disease.

  16. Adenomatoid odontogenic tumor, an uncommon tumor

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    K Vasudevan

    2012-01-01

    Full Text Available Here we report a case of adenomatoid odontogenic tumor (AOT in the maxilla in a young girl aged 14 years and its surgical management. We also review the literature and variations in the nomenclature and classifications of this interesting tumor. The review of literature gives an interesting picture regarding terminologies in the past and dilemma in classifying this tumor. The introduction of the name adenomatoid odontogenic tumour has resulted in the simpler and fruitful surgical management like enucleation and curettage with no reports of recurrences. In the past, similar lesion with the terminology like adeno ameloblastoma has resulted in unnecessary mutilating surgery. The conflicting views whether the lesion is being neoplasm or an anomalous hamartomatous growth is also being discussed.

  17. INTRAOSSEOUS AND EXTRAOSSEOUS VARIANTS OF DENTINOGENIC GHOST CELL TUMOR: TWO CASE REPORTS

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    Merva SOLUK TEKKESIN

    2015-01-01

    Full Text Available This paper aims to present both intraosseous and extraosseous variant of dentinogenic ghost cell tumor as well as a review of the literature. An 11-year old female patient presented a swelling and pain in the molar area of the mandible and a 15-year-old female patient reported a complaint of swelling in the right vestibular region of teeth 12 and 13(FDI 2-digit classification system. Microscopic examinations showed similar features which characterized by ameloblastoma-like islands of epithelial cells, containing numerous ghost cells. The patients have been disease-free for one year. This paper aims to describe this rare tumor and to increase the number of cases in the literature to better understand its biologic behavior and treatment options.

  18. Traumatic bone cyst suggestive of large apical periodontitis.

    Science.gov (United States)

    Rodrigues, Cleomar Donizeth; Estrela, Carlos

    2008-04-01

    This case report shows the importance of establishing the correct diagnosis to provide the appropriate treatment options The traumatic bone cyst is a pseudocyst, usually asymptomatic and found by a routine radiographic examination. Unicystic radiolucency is almost always observed, which can involve the periradicular area of teeth, simulating an inflammatory lesion of endodontic origin. Differential diagnosis should include other pathologies, such as odontogenic keratocyst, central giant cell granuloma, and unicystic ameloblastoma. Its etiology and pathogenesis are not yet definitely established. In the present study, after review of the medical and dental histories and clinical and radiographic examination of teeth #24-27 (pulpal vitality test showed positive), the primary diagnosis was traumatic bone cyst. The planning was excisional biopsy. After surgical exploration, only one small blood clot was observed in the intraosseous socket, which was carefully curetted and filled with blood. A clinical and radiographic examination after 6 months showed apical formation and pulpal vitality preserved.

  19. Incidental bony pathology when reporting trauma orthopantomograms

    Energy Technology Data Exchange (ETDEWEB)

    Macanovic, M., E-mail: mladenmaca@gmail.co [Derriford Hospital NHS Trust, Plymouth (United Kingdom); Gangidi, S.; Porter, G.; Brown, S.; Courtney, D. [Derriford Hospital NHS Trust, Plymouth (United Kingdom); Porter, J. [Community Dental Service, Plymouth Primary Care Trust, Plymouth, Devon (United Kingdom)

    2010-10-15

    Radiologists frequently report orthopantomograms (OPTs) and other views of the mandible, most often in patients who have suffered facial trauma. These examinations may reveal incidental pathology. It is important that radiologists are aware of the radiological appearances and the clinical significance of these lesions. In this review we will present examples of the more common odontogenic lesions including: radicular cyst, odontogenic keratocyst, dentigerous cyst, ameloblastoma, and also examples of non-odontogenic pathology: bisphosphonate-related osteonecrosis of the jaw (BRONJ) and chronic osteomyelitis. Although some of the lesions will require computed tomography (CT) or magnetic resonance imaging (MRI) for further lesion characterization and evaluation of the surrounding tissues, we are going to focus on the plain film appearances. We will also briefly discuss the pathogenesis, epidemiology, and treatment of these lesions.

  20. Creating an effective PBL case in oral and maxillofacial surgery at a Chinese dental school: a dental education primer.

    Science.gov (United States)

    Zheng, Jia Wei; Zhang, Shan Yong; Yang, Chi; Zhang, Zhi Yuan; Shen, Guo Fang

    2011-11-01

    Problem-based learning (PBL) is a widely accepted educational method centered on the discussion and learning that emerge from a clinically based problem; however, little has been reported on the details of PBL case-writing in the dental education literature. This article outlines some principles of writing a PBL case as it is practiced at a Chinese dental school and presents, as an example, an actual case based on a clinical problem (ameloblastoma of the jaw) intended to provide a learning focus for predoctoral dental students. A good PBL case should allow for progressive, interdependent actions to be taken in the evaluation and overall management of the patient in context and should trigger inquiry and discussion among students in both the basic sciences (anatomy, physiology, biochemistry, pharmacology, pathophysiology, etc.) and related clinical sciences. The epidemiological, sociological, and ethical considerations related to each problem should also be emphasized as an essential component of effective health care provision.

  1. Differential diagnosis of tumors of the mandible and maxilla: radiological aspects

    International Nuclear Information System (INIS)

    Isberner, Rony Klaus; Nagazava, Marcio M.; Chiang, Jeng Tyng; Goncalves, Marcelo; Dib, Luciano L.

    1999-01-01

    The radiolucent lesions of the maxilla and jaw can present similar features, such as location, proximity or dental inclusion, insufflative character and density. They are so alike that those signs frequently are not enough for the diagnosis. Among those lesions, we present follicular cysts, ameloblastomas, odontogenic keratocysts, central giant cell lesion, neurofibroma, mucoepydermoid carcinoma and hemangioma, examined with panoramic X-rays, computed tomography and in a specific case, a SPECT for the jaw, with red blood cells- 99m Tc. The objective of this work is to demonstrate in a illustrative way, the radiographic features of some of the radiolucent lesions of the maxilla and jaw, whose differential diagnosis becomes sometimes very difficult, but can be achieved through signs that are more compatible with certain lesions. (author)

  2. Adenomatoid odontogenic tumor in mandibular region

    International Nuclear Information System (INIS)

    Sanchez Cabrales, Ernesto; Vila Morales, Dadonim; Felipe Garmendia, Angel Mario; Serra Ortega, Alain; Torres Gomez de Cadiz, Alma

    2010-01-01

    The adenomatoid odontogenic tumor is an uncommon neoplasm derivative of the odontogenic epithelium containing canalicular structures with inductor modifications of variable intensity in the conjunctival tissue. It is a slow growth lesion and no much invasive but that may to be similar to other odontogenic lesions more aggressive including the dentigerous cyst and the ameloblastoma among others. Its classical location (upper canine area) guides us to diagnosis and its duct histological pattern is very typical of this tumor. Other tumors included in this group are the ameloblastic fibroma, the ameloblastic odontoma, the calcified odontogenic cyst and composed and complex odontomas. This group of lesions may or not to have formations of hard tissue inside. Thus, authors present the case of a patient presenting with this type of tumor making a histopathology study, a literature review on this benign odontogenic tumor and its clinical radiographic features, treatment, as well as the differential diagnoses to be into account. (author)

  3. A multilocular radiolucency of mandible as the first evidence of multiple myeloma: A clinico-radiographic case report

    Directory of Open Access Journals (Sweden)

    Ravi Prakash Sasankoti Mohan

    2014-01-01

    Full Text Available The incidence of multiple myeloma (MM affecting the jaws is 30% and on rare occasions the oral involvement can be the first indication of the disease. Authors report a case of MM in a 40-year-old woman who presented with a multilocular radiolucent lesion in the left mandible initially mistaken as an ameloblastoma. Conventional radiographs revealed a multilocular lesion on the molar region. Computed tomography (CT and 3 dimensional CT revealed lytic, space occupying lesion perforating the inferior cortex. Magnetic resonance imaging (MRI revealed a hypointense lesion on T1 weighted image and hyperintense lesion on T2 weighted image. Histopathological and lab investigations lead to the diagnosis of MM. MRI is superior in depicting the size of the lesion as compared to CT and conventional radiographs.

  4. Two cases report of Calcifying Odontogenic Cyst

    International Nuclear Information System (INIS)

    Lee, Byung Do; Lee, Wan; Paeng, Jun Young; Lee, Jun; Choi, Moon Ki; Son, Hyun Jin

    2009-01-01

    The calcifying odontogenic cyst (COC) is a rare disorder of the jaws and shows various radiographic features. The purpose of this study is to describe the different radiographic appearances of 2 cases of COC. Case 1 was located in the posterior maxilla extending into maxillary sinus, showing unilocular radiolucency with a well-defined margin. Cortical bone expansion and thinning were prominent. Root resorption of adjacent teeth was apparent. Case 2 showed unilocular radiolucency with a calcified material. Calcification was supposed to be dystrophic dental hard structures, detected at the periphery of the lesion. Ghost cell and proliferation of ameloblastoma-like tissues were common features for these two lesions on histopathological findings. This reports presented common and atypical radiographic features of the COC.

  5. Odontogenic cysts, odontogenic tumors, fibroosseous, and giant cell lesions of the jaws.

    Science.gov (United States)

    Regezi, Joseph A

    2002-03-01

    Odontogenic cysts that can be problematic because of recurrence and/or aggressive growth include odontogenic keratocyst (OKC), calcifying odontogenic cyst, and the recently described glandular odontogenic cyst. The OKC has significant growth capacity and recurrence potential and is occasionally indicative of the nevoid basal cell carcinoma syndrome. There is also an orthokeratinized variant, the orthokeratinized odontogenic cyst, which is less aggressive and is not syndrome associated. Ghost cell keratinization, which typifies the calcifying odontogenic cyst, can be seen in solid lesions that have now been designated odontogenic ghost cell tumor. The glandular odontogenic cyst contains mucous cells and ductlike structures that may mimic central mucoepidermoid carcinoma. Several odontogenic tumors may provide diagnostic challenges, particularly the cystic ameloblastoma. Identification of this frequently underdiagnosed cystic tumor often comes after one or more recurrences and a destructive course. Other difficult lesions include malignant ameloblastomas, calcifying epithelial odontogenic tumor, squamous odontogenic tumor, and clear-cell odontogenic tumor. Histologic identification of myxofibrous lesions of the jaws (odontogenic myxoma, odontogenic fibroma, desmoplastic fibroma) is necessary to avoid the diagnostic pitfall of overdiagnosis of similar-appearing follicular sacs and dental pulps. Fibroosseous lesions of the jaws show considerable microscopic overlap and include fibrous dysplasia, ossifying fibroma, periapical cementoosseous dysplasia, and low-grade chronic osteomyelitis. The term fibrous dysplasia is probably overused in general practice and should be reserved for the rare lesion that presents as a large, expansile, diffuse opacity of children and young adults. The need to use clinicopathologic correlation in assessing these lesions is of particular importance. Central giant cell granuloma is a relatively common jaw lesion of young adults that has an

  6. Clinical and Radiographic Study of Benign Odontogenic Tumors in the Jaws

    International Nuclear Information System (INIS)

    Kim, Gyung Yae; Park, Chang Seo

    1989-01-01

    The author observed and analyzed the age, sex, chief complaint and radiographic finding of sixty-one cases of benign odontogenic tumors seen in Yonsei Medical Center, for the period of Jan. 1979 to Aug. 1989. The results were as follows: 1. Benign odontogenic tumors of 61 cases included 52 cases (85.3%) of ameloblastoma and odontoma, and 9 cases of other lesions. Radiographically, the border of the lesions were well-defined. 2. Ameloblastoma constituting twenty-seven cases (44.3%) occurred the average age of 31.1 years and had a 3:1 male predominance. The most common complaint was swelling (20 cases, 74.0%) and followed by pain (13 cases, 48.2%). Radiographically, the most common site was mandibular body area (74.0%) and the lesions were mainly multiocular radiolucency; in 17 cases (63.0%) and unilocular radiolucent lesion were seen in 10 cases (37.0%). 16 cases (59.3%) showed the resorption of roots of adjacent teeth. 3. Odontoma constituting twenty-five cases (41.0%) discovered at the average age of 16.9 years and had a 3:2 male predominance. The most common complaint was delayed eruption of tooth (8 cases, 31.0%) and 7 cases (27.0%) detected on a routine radiograph of the area. Radiographically, 17 cases (68.0%) were of compound type and 8 were of complex variety and compound odontomas were common in the anterior maxilla, whereas complex odontomas occurred more frequently in the posterior mandible. 19 cases (76.0%) showed the impaction of adjacent teeth.

  7. Clinical and Radiographic Study of Benign Odontogenic Tumors in the Jaws

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    Kim, Gyung Yae; Park, Chang Seo [Dept. of Oral Radiology, College of Dentistry, Yonsei University, Seoul (Korea, Republic of)

    1989-11-15

    The author observed and analyzed the age, sex, chief complaint and radiographic finding of sixty-one cases of benign odontogenic tumors seen in Yonsei Medical Center, for the period of Jan. 1979 to Aug. 1989. The results were as follows: 1. Benign odontogenic tumors of 61 cases included 52 cases (85.3%) of ameloblastoma and odontoma, and 9 cases of other lesions. Radiographically, the border of the lesions were well-defined. 2. Ameloblastoma constituting twenty-seven cases (44.3%) occurred the average age of 31.1 years and had a 3:1 male predominance. The most common complaint was swelling (20 cases, 74.0%) and followed by pain (13 cases, 48.2%). Radiographically, the most common site was mandibular body area (74.0%) and the lesions were mainly multiocular radiolucency; in 17 cases (63.0%) and unilocular radiolucent lesion were seen in 10 cases (37.0%). 16 cases (59.3%) showed the resorption of roots of adjacent teeth. 3. Odontoma constituting twenty-five cases (41.0%) discovered at the average age of 16.9 years and had a 3:2 male predominance. The most common complaint was delayed eruption of tooth (8 cases, 31.0%) and 7 cases (27.0%) detected on a routine radiograph of the area. Radiographically, 17 cases (68.0%) were of compound type and 8 were of complex variety and compound odontomas were common in the anterior maxilla, whereas complex odontomas occurred more frequently in the posterior mandible. 19 cases (76.0%) showed the impaction of adjacent teeth.

  8. Distribution of mast cells in benign odontogenic tumors.

    Science.gov (United States)

    de Assis Caldas Pereira, Francisco; Gurgel, Clarissa Araújo Silva; Ramos, Eduardo Antônio Gonçalves; Vidal, Manuela Torres Andion; Pinheiro, Antônio Luiz Barbosa; Jurisic, Vladimir; Sales, Caroline Brandi Schlaepfer; Cury, Patrícia Ramos; dos Santos, Jean Nunes

    2012-04-01

    The aim of this study was to investigate the presence of mast cells in a series of odontogenic tumors. Forty-five cases of odontogenic tumors were investigated using immunohistochemistry for mast cell triptase, and differences between groups were statistically evaluated. Mast cells were present in 96% of odontogenic tumors. Mast cells present in solid ameloblastoma were observed in the tumor stroma surrounding more solid and follicular epithelial islands, with or without squamous metaplasia. The odontogenic mixoma showed few mast cells. In odontogenic tumors with a cystic structure, the mast cells were distributed throughout all areas of the lesions, mainly in keratocystic odontogenic tumor. In addition, the total density of mast cells between all odontogenic tumors showed no significant difference (p > 0.05). A greater mast cells distribution was found in keratocystic odontogenic tumor in relation to adenomatoid odontogenic tumor (p < 0.01), and when the unicystic ameloblastoma and keratocistic odontogenic tumor were compared to the odontogenic myxoma (p < 0.05). Syndrome keratocystic odontogenic tumor showed a higher mean of mast cells when compared with the other tumors of the sample. Mast cells values presented by syndrome keratocystic odontogenic tumor were significantly greater than those of the sporadic keratocystic odontogenic tumor that were not associated with the syndrome (p = 0.03). Mast cells are probably one of the major components of the stromal scaffold in odontogenic tumors. We found significant differences of mast cells between syndrome nonsyndrome keratocystic odontogenic tumors, although their distribution did not seem to have any influence on the biologic behavior of benign odontogenic tumors.

  9. Pattern of histologically diagnosed orofacial tumor and disparity in number managed in a Nigerian University Teaching Hospital: A 5 years review

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    Ramat Oyebunmi Braimah

    2017-01-01

    Full Text Available Background: Orofacial tumors are tumors that can affect any tissue in the oral and maxillofacial region. They constitute a major health problem in Africa because of late presentation. Aim: This was a retrospective study designed to determine the pattern of histologically diagnosed orofacial tumor and those actually managed. Materials and Methods: The department of Oral and Maxillofacial Surgery and Oral Pathology of a University Teaching Hospital in South West Nigeria. Histological diagnoses file and theater register booklet was retrieved from 2010 to 2014. Age, sex, diagnosis, and occupation of the patients were extracted. Data were analyzed using SPSS version 16 (SPSS 16 Inc., Chicago, IL, USA. Results were presented as descriptive frequencies (%. The value of P < 0.05 was considered statistically significant. Results: Of the 188 data analyzed, 98 (52.1% were females, whereas 90 (47.9% were males with male: female ratio 1:1.08. Age groups 21–30 and 31–40 constitute the highest age group with tumor presentation (41 [21.8%]. Most of the patients were traders (62 [33%]. Plexiform ameloblastoma was the most common benign tumor (23 [12.2%], whereas moderately differentiated squamous cell carcinoma was the most common malignant tumor (9 [4.8%]. Only 58 (30.9% were treated while 91 (48.4% were not treated and only 39 (20.7% were referred. Conclusion: Ameloblastoma was the most common odontogenic tumor while moderately differentiated squamous cell carcinoma was the most common malignant lesion. Most of the diagnosed tumors were not treated and reason is due to low financial status of the patients. Negligence on the part of patient could also be a contributing factor.

  10. Evaluation of mandibular bone involvement by oral malignant tumors using scintigraphy of Tc-99m MDP and Ga-67 citrate. Intensely invaded cases

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    Katada, Yoshiaki; Tsuchimochi, Makoto; Kato, Joji

    1987-12-01

    Ten patients with intensely invaded malignant tumors at the mandibles were studied with bone scintigraphy (Tc-99m MDP) and Ga-67 citrate scintigraphy. All patients were classified by type of accumulation of both radionuclide pharmaceuticals. In almost all patients with invaded malignant tumors, bone scintigraphy revealed decreasing uptake in the center of the lesion and increasing uptake in the peripheral region. Ga-67 citrate scintigram showed increasing concentrated accumulation which was smaller than the area of Tc-99m MDP uptake in almost all cases. These types of accumulation were compared with those of other oral and maxillo-facial diseases. The patterns of accumulation of both radionuclide pharmaceuticals in ameloblastoma (3 cases), radicular cyst (3 cases), bone fracture (2 cases), diffuse sclerosing osteomyelitis (2 cases), and one sagittal ramus osteotomy case were different from that of bone involvement of malignant tumor cases. From the types of accumulation, we may be able to differentiate the oral and maxillo-facial diseases to some extent. In addition, the intensity of accumulation of Tc-99m MDP was measured by using bone to soft tissue ratio (4 hrB/St ratio). The mean 4 hrB/St ratio was higher in moth-eaten absorbed type than in permeated type of resorption in roentgenogram. However, we could not obtain a definitive conclusion because of too few cases. The mean 4 hrB/St ratio in the bone involvement by malignant tumor was higher than that in radicular cyst, and lower than that of diffuse sclerosing osteomyelitis. Unfortunately, we could not differentiate each disease of the involved malignant tumor, ameloblastoma, or bone fracture by only the 4 hrB/St ratio. The Tc-99m MDP accumulated beyond the region where the radiolucencies exist in roentgenograms. We consider that both scintigraphies have a great role in planning surgical treatment, especially in selecting osteotomy.

  11. Fibroma ameloblástico versus quiste folicular hiperplásico Ameloblastic fibroma versus hyperplastic follicular cyst

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    J. Azúa-Romeo

    2004-06-01

    Full Text Available Resumen: El fibroma ameloblástico (FA es un tumor odontogénico mixto, compuesto por tejido mesenquimal y epitelio odontogénico, representando el 2% de los tumores odontogénicos, mientras que el quiste dentígero o folicular (QF, el segundo quiste odontogénico en frecuencia, está compuesto exclusivamente por tejido conjuntivo laxo (mesénquima, si bien, el saco fibroso puede contener restos de epitelio odontogénico incluidos, dando lugar a una imagen histológica muy similar. La importancia de esta diferenciación radica en el tratamiento, que es ligeramente más agresivo en el FA y en el comportamiento biológico, ya que el FA puede derivar en un sarcoma ameloblástico, y el QF puede evolucionar hacia ameloblastoma y más raramente carcinoma mucoepidermoide.Abstract: Ameloblastic fibroma (AF is a mixed odontogenic tumor composed of mesenchymal tissue and odontogenic epithelium, accounting for 2% of all odontogenic tumors, while dentigerous or follicular cyst (FQ, second odontogenic cyst in frequency, is formed exclusivelly by soft conective tissue (mesenchyme. Nevertheless, hiperplastic fibrous sac may contain rests of odontogenic epithelium, showing a similar histologic pattern. The importance of an adequate differentiation lies in the treatment, which is slightly aggresive for AF, and regarding the biological behaviour of both lesions, since AF might become in an ameloblastic sarcoma, while over an FQ could develope an ameloblastoma or even a mucoepidermoid carcinoma.

  12. Odontogenic tumors in Nigerian children and adolescents- a retrospective study of 92 cases

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    Adeyemo Wasiu L

    2004-11-01

    Full Text Available Abstract Background Tumours arising from odontogenic tissues are rare and constitute a heterogenous group of interesting lesions. The aim of this study was to determine the relative frequency of odontogenic tumors (OT among Nigerian children and adolescents 19 years or younger. Patients and methods The histopathology records were retrospectively reviewed for all the tumors and tumor-like lesions of the oral cavity and the jaws seen in children and adolescents ≤ 19 years seen between January 1980 and December 2003. Hematoxylin and eosin-stained sections were re-evaluated and the diagnosis in each case was confirmed or modified according to World Health Organization (WHO classification, 1992; and were subjected to analysis of age, sex, site of tumor and histopathologic type. Results A total of 477 tumors and tumor-like lesions were seen in patients ≤ 19 years during the period of the study. Of these, 92 (19.3% were odontogenic tumors. Benign odontogenic tumors constituted 98.9% of the cases seen, while only 1 case (1.1% of malignant variety was seen during the period. The mean (SD age of patients was 14.9 (± 3.1 years (range, 4–19 years. Male-to-female ratio was 1:1; and mandible-to-maxilla ratio was 2.7:1. OT's were most frequently seen in patients aged 16–19 years (46.7% and the least number (2.2% were found in patients aged 0–5 years. Among nine histologic types of OT seen, ameloblastoma (48.9%, adenomatoid odontogenic tumor (19.6% and odontogenic myxoma (8.7% were predominant. Multicystic/solid and unicystic variants of ameloblastoma were diagnosed in 40 (89% and 5 (11% cases respectively. Conclusions Odontogenic tumors are relatively common in children and adolescents in Nigeria. One out of every 5 children and adolescents with tumors and tumor-like lesions of oral cavity and the jaws seen in this study had a diagnosis of odontogenic tumor.

  13. Tumours and tumour-like lesions of the lower face at Korle Bu Teaching Hospital, Ghana--an eight year study.

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    Parkins, Grace E A; Armah, George; Ampofo, Patrick

    2007-05-07

    The oro-facial region including the jawbones, the maxilla and mandible and related tissues can be the site of a multitude of neoplastic conditions. These tumours have a predilection for the entire facial region; however, odontogenic tumours tend to affect the mandible more than the maxilla, especially, in West African children. We report results from a retrospective study spanning eight years on the frequency, clinical presentation, sites and character of lower face tumours seen in the main referral hospital in Ghana. Records of consecutive patients of all age and sex seen by the first author's team at the Department of Oral and Maxillofacial Surgery, Korle-Bu Teaching Hospital with tumours affecting the lower part of the face from January 1996 to December 2003 were retrieved, coded and entered into a database. The data were then analyzed by age, sex, presenting signs and symptoms, site of lesion, and their histology. A total of 394 patients with oro-facial swellings were retrieved from the registry out of which 210 had lower face tumour and tumour-like lesions. The complete data set was obtained for 171 patients, comprising 99 (58%) males and 72 (42%) females. The most common clinical presenting features were mandibular facial swelling (63%), intra-oral swelling (55%), pain (41%) and ulceration (29%). The tumours were predominantly found in the right (43%), anterior (19%) and left (18%) aspects of the lower face. The remainder making up 20% were found in the floor of the mouth, tongue and lips. Seventy eight (45.6%) of the patients presented with lesions that were classified as malignant of which 54 (62%) were diagnosed as squamous cell carcinoma (SCC). Sixty-two (36.3%) had benign odontogenic tumours and thirty-one (18.1%) had non-odontogenic tumour-like lesions. Fifty-four (62%) of malignant tumours were squamous cell carcinoma; 58 (93.6%) of the benign odontogenic tumours were classified as ameloblastoma. The mean age at presentation of all lesions was 40

  14. Tumours and tumour-like lesions of the lower face at Korle Bu Teaching Hospital, Ghana – an eight year study

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    Ampofo Patrick

    2007-05-01

    Full Text Available Abstract Background The oro-facial region including the jawbones, the maxilla and mandible and related tissues can be the site of a multitude of neoplastic conditions. These tumours have a predilection for the entire facial region; however, odontogenic tumours tend to affect the mandible more than the maxilla, especially, in West African children. We report results from a retrospective study spanning eight years on the frequency, clinical presentation, sites and character of lower face tumours seen in the main referral hospital in Ghana. Patients and methods Records of consecutive patients of all age and sex seen by the first author's team at the Department of Oral and Maxillofacial Surgery, Korle-Bu Teaching Hospital with tumours affecting the lower part of the face from January 1996 to December 2003 were retrieved, coded and entered into a database. The data were then analyzed by age, sex, presenting signs and symptoms, site of lesion, and their histology. Results A total of 394 patients with oro-facial swellings were retrieved from the registry out of which 210 had lower face tumour and tumour-like lesions. The complete data set was obtained for 171 patients, comprising 99 (58% males and 72 (42% females. The most common clinical presenting features were mandibular facial swelling (63%, intra-oral swelling (55%, pain (41% and ulceration (29%. The tumours were predominantly found in the right (43%, anterior (19% and left (18% aspects of the lower face. The remainder making up 20% were found in the floor of the mouth, tongue and lips. Seventy eight (45.6% of the patients presented with lesions that were classified as malignant of which 54 (62% were diagnosed as squamous cell carcinoma (SCC. Sixty-two (36.3% had benign odontogenic tumours and thirty-one (18.1% had non-odontogenic tumour-like lesions. Fifty-four (62% of malignant tumours were squamous cell carcinoma; 58 (93.6% of the benign odontogenic tumours were classified as ameloblastoma. The

  15. Expression of podoplanin and TGF-beta in glandular odontogenic cyst and its comparison with developmental and inflammatory odontogenic cystic lesions.

    Science.gov (United States)

    Alaeddini, Mojgan; Eshghyar, Nosratollah; Etemad-Moghadam, Shahroo

    2017-01-01

    The number of studies investigating the immunohistochemical characteristics of glandular odontogenic cysts (GOCs) is limited, due to its rarity. TGF-beta has been suggested to induce podoplanin expression in some lesions. We aimed to evaluate and compare podoplanin and TGF-beta expression in GOC and other odontogenic cystic lesions. A total of 43 samples including five GOCs, 10 dentigerous cysts (DCs), eight unicystic ameloblastoma (UAs), and 20 radicular cysts (RCs) were selected and subjected to immunohistochemical staining using monoclonal antibodies against podoplanin and TGF-beta. Kruskal-Wallis test and Mann-Whitney U-test were used for statistical analysis along with Bonferroni for adjusting P-values (P < 0.05). Podoplanin immunoreactivity was observed in 80%, 70%, and 100% of DCs, RCs, and UAs, respectively, while none of the GOCs were positive for this marker (P = 0.004). Significant differences were only found in the GOC specimens. TGF-beta positivity occurred in the capsule and epithelium of all GOCs and DCs, while RCs and UAs demonstrated different expression percentages in the capsular and epithelial tissues. Epithelial TGF-beta showed significant differences among the studied lesions (P = 0.007) with the main difference found between DCs with RCs and DCs with UAs. Lack of podoplanin expression might be involved in the characteristic histologic and behavioral features of GOC, which seems to be unrelated to TGF-beta expression. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  16. WhatsApp is an effective tool for obtaining second opinion in oral pathology practice.

    Science.gov (United States)

    Sarode, Sachin C; Sarode, Gargi S; Anand, Rahul; Patil, Shankargouda; Unadkat, Hemant

    2017-08-01

    The aim of this study was to find out the efficacy of WhatsApp application for obtaining second opinion on histopathological diagnosis in oral pathology practice. A total of 247 cases comprising of 34 different oral pathologies were photomicrographed using smartphone cameras through compound microscopes and sent for second opinion diagnosis (SOD) to 20 different oral pathologists using WhatsApp. Of 4795 (97.06%) total second opinion received, correct SOD were received for 4710 (98.22%) cases. Hundred percent times correct SOD was received for lesions including adenomatoid odontogenic tumor, keratinizing cystic odontogenic tumor, odontome, and dentigerous cyst. Lesions such as myoepithelial carcinoma, osteosarcoma, fibrosarcoma, and intravascular papillary endothelial hyperplasia received less percentage of correct SOD (85.71-75.75%). Correct SOD was obtained for variants of ameloblastoma (99.01%), grading of epithelial dysplasia (87.54%), and squamous cell carcinoma (95.26%). A positive correlation was observed between correct SOD and age (P = 0.0143) and experience (P = 0.0189) of the pathologist. The time taken for giving second opinion by the pathologists ranged from 81.98 ± 32.89 to 90.72 ± 38.88 min. Smartphone camera is a handy and efficient tool in capturing photomicrographs from the compound microscope. Transfer of such photomicrograph via WhatsApp is an effective and convenient approach in procuring second opinion on histopathological diagnosis of oral pathologies. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  17. A study on the mixed jaw lesions associated with teeth

    International Nuclear Information System (INIS)

    Nah, Kyung Soo

    2000-01-01

    1. Retrospectively evaluate the accuracy of tentative diagnosis or impression from the clinico-radiographic materials of jaw lesions which showed mixed lesions associated with teeth. 2. To observe the diagnostic importance of the calcified part of the lesions which appear as radiopaque areas. 14 cases of jaw lesions which showed mixed lesions associated with teeth were reviewed. These lesions were mostly diagnosed as adenomatoid odontogenic tumors (6 cases) or calcifying odontogenic cysts with (4 cases) or without odontomas (4 cases). The calcified elements of the lesions which demonstrated various sizes and patterns of radiopaque shadows resembled odontoid tissues in some cases but could not be defined in some other cases radiographically. The final histopathologic diagnosis confirmed adenomatoid odontogenic tumors in 4 of the 6 cases. The remaining 2 cases turned out to be odontoma and ameloblastic fibroodontoma. The 4 cases of calcifying odontogenic cysts with odontomas were correct in 3 cases but remaining 1 case was just odontoma. The 4 cases of calcifying odontogenic cysts were proved to be odontogenic keratocyst, calcified peripheral fibroma, unicystic ameloblastoma and squamous cell carcinoma. The diagnostic accuracy of the adenomatoid odontogenic tumors and calcifying odontogenic cysts were high when the lesions show typical appearance. The calcifications which show radiopaque areas could be odontomas or dystrophic calficifations or remnants of bone fragments from resorption.

  18. Case report and literature review of adamantinoma of long bone

    International Nuclear Information System (INIS)

    Gao Zhenhua; Chen Yingming; Guan Jian; Meng Junfei

    2010-01-01

    Objective: To analyze the clinical, imaging and pathological features of adamantinoma of long bone to aid preoperative diagnosis. Methods: A case of adamantinoma of long bone was reported and the related literature was reviewed. Results: Literature review showed that long bone ameloblastoma was more common in young adults older than 20 years of age (86.3%) and occurred mainly in the tibial shaft (90%). The clinical, imaging and pathological features in our patient were consistent with those reported in the literature. Among 39 cases in the Chinese literature, radiographic findings included uni-or multi-cystic bone destruction with well-defined borders (87.2%), bone expansion (61.8%), partial loss of cortical bone (26.5%), bone sclerosis (38.2%), and no periosteal new bone reaction (100%). Pathology showed epithelial and fibrous differentiation. Conclusion: Adamantinoma of long bone should be considered in patients older than 20 years of age with tibial lesions that have imaging characteristics of benign bone tumors or some malignant features. (authors)

  19. Immediate reconstruction of a large mandibular defect of locally invasive benign lesions (a new method).

    Science.gov (United States)

    Shirani, Gholamreza; Arshad, Mahnaz; Mohammadi, Farnoush

    2007-11-01

    Locally invasive benign tumor and large lesions such as ameloblastoma, giant cell granuloma, odontogenic keratocyst, and odontogenic myxoma are a benign, invasive, lesions of the jaws that predominantly affects the mandible. Despite the benign nature of these lesions, there is a high rate of local recurrence after curettage, which usually requires resection. The traditional surgical approach for resection of these lesions, via either mandibulotomy or mandibulectomy is extraoral approach which is associated with significant functional and esthetic sequelae. A case series is presented here in which less invasive and intraoral approach. An intraoral approach provides wide and fast access to the mandible. This approach represents a less invasive alternative that provides access to the mandible for curative resection of benign tumors with minimal postoperative sequelae. At 5 years follow-up, there were minimal functional and esthetic defects. We explored the use of the less invasive and more esthetic incision as an approach to resection and reconstruction of the mandible. It is our belief that these concerns have been best addressed by the minimally invasive procedure used in this report. This approach resulted in a minimal esthetic and functional defect even though a massive mandibular resection was performed.

  20. Fine-needle aspiration biopsy of intraosseous jaw lesions.

    Science.gov (United States)

    August, M; Faquin, W C; Ferraro, N F; Kaban, L B

    1999-11-01

    This study assessed diagnostic accuracy, determined reasons for error, and evaluated modifications to improve the reliability of fine-needle aspiration biopsy (FNAB) of primary jaw lesions. This was a retrospective review of 32 FNABs of intraosseous jaw lesions performed at the Massachusetts General and Children's Hospital between 1993 and 1998. A consistent, standardized technique was used, and each case was evaluated for 1) adequacy of cells to allow diagnosis, 2) presence of malignant cells, and 3) correlation between FNAB diagnosis and the final histopathology. Material obtained by FNAB was adequate for evaluation in 30 of 32 cases. No complications were reported. Malignant cells were found in 5 of 30 cases. FNAB diagnosis was confirmed by histopathology in all 5 of these specimens (100% accuracy). The FNAB diagnosis of benign lesions was confirmed in 17 of 25 cases (68%). The most common benign lesions were odontogenic cysts, ameloblastomas, and fibro-osseous and giant cell lesions. Incorrect diagnosis was related to lack of architectural context of the FNAB material, sampling of a nonrepresentative part of a large lesion, and inadequate quantity or quality of the aspirate. FNAB is a useful technique to distinguish between malignant and benign intraosseous jaw lesions. Its simplicity, suitability as an outpatient procedure, rapidity of interpretation, and minimal morbidity potentially make it the diagnostic tool of choice in the hospital setting.

  1. Role of FNAC in the diagnosis of intraosseous jaw lesions.

    Science.gov (United States)

    Goyal, Surbhi; Sharma, Sonal; Kotru, Mrinalini; Gupta, Neelima

    2015-05-01

    FNAC of intraosseous jaw lesions has not been widely utilized for diagnosis due to rarity and diversity of these lesions, limited experience and lack of well established cytological features. Aim of the study was to determine the role of FNAC in the diagnosis of intraosseous jaw swellings. 42 patients underwent FNAC over a period of 7 years (2007-2013), of which 37 (88.1%) aspirates were diagnostic. Histopathology correlation was available in 33 cases and diagnostic accuracy of FNAC was calculated. Lesions were categorized into inflammatory 3, cysts/hamartomas 15 and neoplasms 19. Mandibular and maxillary involvement was seen in 21 and 16 patients respectively. Of these, benign cysts and malignant lesions were commonest, accounting for 27% lesions (10 cases) each. One case of cystic ameloblastoma was misdiagnosed as odontogenic cyst on cytology. Overall, sensitivity and specificity of FNAC were 94.7% and 100% respectively with a diagnostic accuracy of 97.3%. Definitive categorization of giant cell lesions, fibro-osseous lesions, odontogenic tumors and cystic lesions was not feasible on FNAC. FNAC is a simple, safe and minimally invasive first line investigation which can render an accurate preoperative diagnosis of intraosseous jaw lesions, especially the malignant ones in the light of clinic-radiological correlation.

  2. Immunoreactivity of granular cell lesions of skin, mucosa, and jaw.

    Science.gov (United States)

    Regezi, J A; Zarbo, R J; Courtney, R M; Crissman, J D

    1989-10-01

    Granular cell lesions from many different sites share similar light and electron microscopic features. Immunologically, however, these lesions do not appear to be a homogenous group. This study determines the extent of immunologic heterogeneity of granular cell lesions from a wide variety of sites in skin, mucosa, and jaw. Thirty-one granular cell lesions (26 granular cell tumors [GCT] and five other granular cell lesions) from 18 different sites were evaluated immunohistochemically for keratins, vimentin, desmin, muscle actin, ACT, HLA-DR, and S-100 protein. Paraffin-embedded sections were utilized with an avidin-biotin complex immunoperoxidase technique. Except for ameloblastomas, all lesions were negative for keratin and positive for vimentin. All lesions were negative for desmin and actin. Positive ACT reactivity was found in one of seven GCT of tongue, a colonic lesion, a nose lesion, and a granular cell ameloblastic fibroma. All lesions were positive for HLA-DR except a few in which fixation appeared inadequate. S-100 immunoreactivity was found in all lesions except the congenital epulis, a GCT of the skin of the nose, a colonic lesion, and the odontogenic tumors. The antigenic profile of GCT of skin and mucosa is consistent with Schwann cell origin. However, some GCT and other granular cell lesions appear to be derived from macrophages, epithelial cells, or other cells. The expression of HLA-DR by granular cells is believed to be unrelated to cellular origin but rather to some common immunologic function.

  3. Pleomorphic Adenoma of the Palate in a 10-year-old Child: A Case Report

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    SH Tabatabaei

    2016-03-01

    Full Text Available Salivary gland tumors  are  uncommon  within  children and  when  they do arise,  they  mainly  affect  the  major  salivary glands. Minor salivary gland  tumors  are  rare  in  children, which are  responsible for less than 10% of all the cases. Pleomorphic adenoma is the most common tumor of  the salivary glands in the all ages. A 10-year-old boy referred to the dentistry clinic with a swelling in the palate of adjacent unerupted right maxillary second molar. In sampling by curettage, clinical pathologist reported ameloblastoma. The patient was referred to the dental school of Shahid Sadoughi University of Medical Sciences for consultation. Second  microscopic analysis showed a benign pleomorphic adenoma that was confirmed by special staining periodic acid-Schiff (PAS, immunohistochemistry cytokeratin(CK, and S100 staining. Pleomorphic adenoma of minor salivary gland of children should be considered as a differential diagnosis of intraoral swelling in the palate.

  4. Radiological and histopathological study of benign tumors of the mandible

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, Seon Young; Baek, Seung Yon; Choi, Kyung Hee; Suh, Jeung Soo; Rhee, Chung Sik; Kim, Hee Seup [Ewha Womans University College of Medicine, Seoul (Korea, Republic of)

    1984-06-15

    Benign tumors of the mandible are uncommon lesions. That were classified into odontogenic tumors and nonodontogenic tumors. Author reviewed the radiological evaluation and pathological microscopic finding from 33 benign tumors of the mandible that were confirmed by the biopsy during last 10 years in Dental Clinics, Ewha Womans University Hospital and Seoul National University Dental Hospital. Following results were obtained; 1. Benign tumors of the mandible were classified into odontogenic (66.7%) and non-odontogenic tumors (33.3%). 2. The range of the age distribution was between 6 years and 67 years old. The commonest age group was the second decade (39.4%). 3. There was no difference to sex distribution. 4. The most frequent location was the body of the mandible (42.4%). 5. Radiographic findings were relatively characteristic in odontogenic tumors rather than non-odontogenic tumors. 1) Radiolucent cystic lesions-ameloblastoma, odontogenic myxoma, odontogenic fibtoma, aneurysmal bone cyst and neurofibroma. 2) Radiopaque mass lesions-odontoma, cementoma and osteoma. 3) Mixed patterns-ossifying fibroma, cementifying fibroma, calcifying odontogenic epithelial tumor and hemangioma. It was concluded that the radiographic examination was of value to diagnose the benign tumors of the mandible in symptomless patients.

  5. Radiological and histopathological study of benign tumors of the mandible

    International Nuclear Information System (INIS)

    Yoo, Seon Young; Baek, Seung Yon; Choi, Kyung Hee; Suh, Jeung Soo; Rhee, Chung Sik; Kim, Hee Seup

    1984-01-01

    Benign tumors of the mandible are uncommon lesions. That were classified into odontogenic tumors and nonodontogenic tumors. Author reviewed the radiological evaluation and pathological microscopic finding from 33 benign tumors of the mandible that were confirmed by the biopsy during last 10 years in Dental Clinics, Ewha Womans University Hospital and Seoul National University Dental Hospital. Following results were obtained; 1. Benign tumors of the mandible were classified into odontogenic (66.7%) and non-odontogenic tumors (33.3%). 2. The range of the age distribution was between 6 years and 67 years old. The commonest age group was the second decade (39.4%). 3. There was no difference to sex distribution. 4. The most frequent location was the body of the mandible (42.4%). 5. Radiographic findings were relatively characteristic in odontogenic tumors rather than non-odontogenic tumors. 1) Radiolucent cystic lesions-ameloblastoma, odontogenic myxoma, odontogenic fibtoma, aneurysmal bone cyst and neurofibroma. 2) Radiopaque mass lesions-odontoma, cementoma and osteoma. 3) Mixed patterns-ossifying fibroma, cementifying fibroma, calcifying odontogenic epithelial tumor and hemangioma. It was concluded that the radiographic examination was of value to diagnose the benign tumors of the mandible in symptomless patients

  6. A study on the mixed jaw lesions associated with teeth

    Energy Technology Data Exchange (ETDEWEB)

    Nah, Kyung Soo [Dept. of Dental Radiology, College of Dentistry, Pusan National University, Pusan (Korea, Republic of)

    2000-03-15

    1. Retrospectively evaluate the accuracy of tentative diagnosis or impression from the clinico-radiographic materials of jaw lesions which showed mixed lesions associated with teeth. 2. To observe the diagnostic importance of the calcified part of the lesions which appear as radiopaque areas. 14 cases of jaw lesions which showed mixed lesions associated with teeth were reviewed. These lesions were mostly diagnosed as adenomatoid odontogenic tumors (6 cases) or calcifying odontogenic cysts with (4 cases) or without odontomas (4 cases). The calcified elements of the lesions which demonstrated various sizes and patterns of radiopaque shadows resembled odontoid tissues in some cases but could not be defined in some other cases radiographically. The final histopathologic diagnosis confirmed adenomatoid odontogenic tumors in 4 of the 6 cases. The remaining 2 cases turned out to be odontoma and ameloblastic fibroodontoma. The 4 cases of calcifying odontogenic cysts with odontomas were correct in 3 cases but remaining 1 case was just odontoma. The 4 cases of calcifying odontogenic cysts were proved to be odontogenic keratocyst, calcified peripheral fibroma, unicystic ameloblastoma and squamous cell carcinoma. The diagnostic accuracy of the adenomatoid odontogenic tumors and calcifying odontogenic cysts were high when the lesions show typical appearance. The calcifications which show radiopaque areas could be odontomas or dystrophic calficifations or remnants of bone fragments from resorption.

  7. Digital image processing of mandibular trabeculae on radiographs

    Energy Technology Data Exchange (ETDEWEB)

    Ogino, Toshi

    1987-06-01

    The present study was aimed to reveal the texture patterns of the radiographs of the mandibular trabeculae by digital image processing. The 32 cases of normal subjects and the 13 cases of patients with mandibular diseases of ameloblastoma, primordial cysts, squamous cell carcinoma and odontoma were analyzed by their intra-oral radiographs in the right premolar regions. The radiograms were digitized by the use of a drum scanner densitometry method. The input radiographic images were processed by a histogram equalization method. The result are as follows : First, the histogram equalization method enhances the image contrast of the textures. Second, the output images of the textures for normal mandible-trabeculae radiograms are of network pattern in nature. Third, the output images for the patients are characterized by the non-network pattern and replaced by the patterns of the fabric texture, intertwined plants (karakusa-pattern), scattered small masses and amorphous texture. Thus, these results indicates that the present digital image system is expected to be useful for revealing the texture patterns of the radiographs and in the future for the texture analysis of the clinical radiographs to obtain quantitative diagnostic findings.

  8. Digital image processing of mandibular trabeculae on radiographs

    International Nuclear Information System (INIS)

    Ogino, Toshi

    1987-01-01

    The present study was aimed to reveal the texture patterns of the radiographs of the mandibular trabeculae by digital image processing. The 32 cases of normal subjects and the 13 cases of patients with mandibular diseases of ameloblastoma, primordial cysts, squamous cell carcinoma and odontoma were analyzed by their intra-oral radiographs in the right premolar regions. The radiograms were digitized by the use of a drum scanner densitometry method. The input radiographic images were processed by a histogram equalization method. The result are as follows : First, the histogram equalization method enhances the image contrast of the textures. Second, the output images of the textures for normal mandible-trabeculae radiograms are of network pattern in nature. Third, the output images for the patients are characterized by the non-network pattern and replaced by the patterns of the fabric texture, intertwined plants (karakusa-pattern), scattered small masses and amorphous texture. Thus, these results indicates that the present digital image system is expected to be useful for revealing the texture patterns of the radiographs and in the future for the texture analysis of the clinical radiographs to obtain quantitative diagnostic findings. (author)

  9. Odontogenic tumors: A review of 675 cases in Eastern Libya

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    Saravana HL Goteti

    2016-01-01

    Full Text Available Aims: The aim of this study was to determine the relative frequency of odontogenic tumors (OTs in an Eastern Libyan population based on the 2005 World Health Organization (WHO classification, and also to compare the actual data with previous studies. Materials and Methods: We retrieved and analyzed 85 OTs from a total of 675 tumors and tumor-like lesions of the oral and perioral structures, for gender, age, tumor site, and frequency. The diagnosis was based on the most recent WHO (2005 classification of OTs. Results: OTs constituted 12.6% of all oral/jaw tumors and tumor-like lesions. Ameloblastoma (28.2% was the most common type, followed by keratocystic odontogenic tumor (25.2% and odontoma (19.9%. The male: female ratio was 1.2:1, and maxilla: mandible ratio 1:2. The mean age of occurrence of tumors was 29 years with a peak incidence between 10 and 40 years. Conclusions: OTs are relatively common lesion in this Libyan Population, but the incidence of tumors is neither similar to Caucasians nor Sub-Saharan population.

  10. Canine oral cavity neoplasias - Brief review

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    João Filipe Requicha

    2015-03-01

    Full Text Available ABSTRACT. Requicha J.F., Pires M. dos A., Albuquerque C.M. & Viegas C.A. [Canine oral cavity neoplasias - Brief review.] Neoplasias da cavidade oral do cão - Breve revisão. Revista Brasileira de Medicina Veterinária, 37(1:41-46, 2015. Faculdade de Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias, Campo Grande, 1749-024 Lisboa, Portugal e Department of Veterinary Sciences, School of Agriculture and Veterinary Sciences, University of Trás-os-Montes e Alto Douro, P.O. Box 1013, 5001-801 Vila Real, Portugal. E-mail: jfrequicha@gmail.com Oral proliferative lesions are relatively common in domestic carnivores but, fortunately, a lot of these lesions are benign. The oral cavity is place of 6% of all tumours in dogs, being the sixth most important localization of neoplasias in this specie. The non-odontogenic tumors arise from structures of the oral cavity, except from dental tissue, and they are mostly malignant. Odontogenic tumors are those originated from the dental structures. In the case of tumors of non-odontogenic, will be described the oral papillomatosis, the melanoma, the squamous cell carcinoma, and the fibrosarcoma. Among the odontogenic tumors, the focus will be on the epulides, ameloblastoma, odontoma and dentigerous cysts.

  11. Odontogenic tumors: A retrospective study of four Brazilian diagnostic pathology centers

    Science.gov (United States)

    da-Costa, Daniela O P.; Maurício, Almir S.; de-Faria, Paulo A S.; da-Silva, Licínio E.; Mosqueda-Taylor, Adalberto

    2012-01-01

    Objective: This article presents the results of a retrospective study of the frequency and classification of odontogenic tumors recorded at four centers of diagnostic pathology in Rio de Janeiro, Brazil. Study Design: All medical records and microscopic slides of odontogenic tumor specimens for the years 1997 to 2007 were retrieved from the files of four services of diagnostic pathology in Rio de Janeiro City. Diagnoses were re-evaluated and the tumors classified according to the latest (2005) World Health Organization Classification of Tumors. Results: A total of 201 odontogenic tumors were found among 15,758 oral biopsies (1.3%). The frequencies of these tumors at the four centers ranged from 0.5% at the National Cancer Institute to 3.3% in a private laboratory. Chi-square analysis revealed statistically significant differences (p<0.05) between the proportions of odontogenic tumors in the studied centers. Of these, 94.5% were benign and 5.5% were malignant. Keratocystic odontogenic tumor (32.3%) was the most frequent lesion, followed by ameloblastoma (29.8%) and odontoma (18.4%). Conclusions: Odontogenic tumors are uncommon in Brazil. Different pathology laboratories reported divergent frequencies of odontogenic tumors, which may reflect institutional specializations and the patient populations served. Key words:Odontogenic tumors, jaw neoplasms, epidemiology, WHO classification. PMID:22143740

  12. Aesthethic and masticatory rehabilitation on post mandibular resection with combination of hollow obturator and hybrid prosthesis

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    Arif Rachman

    2009-06-01

    Full Text Available Background: Replacing tooth lost caused by caries, periodontal disease, trauma and neoplasm including ameloblastoma which requires mandibular resection is important. Purpose: The aim of the study to rehabilitation of post mandibular resection with combination of hollow obturator and hybrid prosthesis. Case: A patient 25 years old, male, for having prosthesis to cover defect due to post right mandibular resection. Case Management: In this presented case, mandibular plate was applied due to spreading defect with losing almost a half body of mandible (class II modification 2 according to cantor and curtis classification. The design of therapy was mandibular obturator using hybrid prosthesis (removable partial denture metal frame and fixed splint crown with precision attachment with hollow obturator. The application was based on several advantages: good aesthetic performance, retention, stability, lighter weight and equal share of vertical load for teeth on non surgical site. The result of control I, II, III, showed that aesthetic performance, masticatory function, speech and swallowing were in good condition. Conclusion: The design of mandibular obturator using hybrid denture with hollow obturator could rehabilitate aesthetic performance, masticatory function, speech and swallowing for patient with post mandibular resection.

  13. Mandibular Defect Reconstruction with the Help of Mirror Imaging Coupled with Laser Stereolithographic Modeling Technique

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    Jing-Wei Lee

    2007-01-01

    Full Text Available With the advent of microsurgery, composite defect in the mandible can be repaired with various forms of osteocutaneous free flaps. However, it is difficult to accurately reconstruct a large defect in the mandible when not enough mandibular reference blueprints remain. This case report describes a large ameloblastoma at the left lower molar region and ascending ramus of the mandible in a 53-year-old male patient. Before surgery, spiral computed tomography scanning of the whole skull of the patient was performed. Using three-dimensional reconstruction and mirror imaging coupled with laser stereolithographic technique, a complete mandibular biomodel with idealized shape was fabricated. A titanium reconstruction plate was made using the biomodel as a guide. The tumor mass together with the left mandible from the second premolar to the condylar head area was resected en bloc. The large mandibular defect was then reconstructed with the precontoured titanium plate and three segments of vascularized fibular bone graft fixed along the plate. The temporomandibular joint was restored with temporalis muscle as an interpositional disc replacement. The complex defect in the mandible was thus repaired with satisfactory functioning and esthetic result. We suggest that with the help of mirror imaging coupled with laser stereolithographic technique, a precontoured titanium plate can be made for the reconstruction of large mandibular defects. [J Formos Med Assoc 2007;106(3:244-250

  14. Parameters in panoramic radiography for differentiation of radiolucent lesions

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    Ricardo Raitz

    2009-10-01

    Full Text Available OBJECTIVE: The aims of this study were to establish parameters in panoramic radiography for interpretation of unilocular radiolucent lesions, and to compare the accuracy of diagnoses given by examiners before and after using these parameters. MATERIAL AND METHODS: In Part I, 12 specialists analyzed 24 images and the diagnostic criteria used by each examiner to make correct diagnoses were used to build a list of basic radiographic parameters for each pathology (ameloblastoma, keratocystic odontogenic tumor, dentigerous cyst, and idiopathic bone cavity. In Part II, this list was used by 6 undergraduate students (Un, 8 recently graduated dentists (D, 3 oral pathologists, 3 stomatologists, 3 oral radiologists, and 3 oral surgeons to diagnose the corresponding pathologies in the other set of 24 panoramic radiographs (T2. The same analysis occurred without using this list (T1. The method of generalized estimating equations (GEE was used in order to estimate the probability of making a correct diagnosis depending on the specialty of the examiner, type of lesion, and moment of the evaluation, T1 or T2 (before or after they had access to the list of parameters, respectively. RESULTS: Higher values were obtained for the probability (GEE of making a correct diagnosis on T2; the group Un presented the highest improvement (14.6 %; no differences between the probabilities were observed either between Un and D, or among the different groups of specialists. CONCLUSIONS: The use of panoramic radiographic parameters did allow improving the diagnostic accuracy for all groups of examiners.

  15. Parameters in panoramic radiography for differentiation of radiolucent lesions.

    Science.gov (United States)

    Raitz, Ricardo; Assunção Júnior, José Narciso Rosa; Correa, Luciana; Fenyo-Pereira, Marlene

    2009-01-01

    The aims of this study were to establish parameters in panoramic radiography for interpretation of unilocular radiolucent lesions, and to compare the accuracy of diagnoses given by examiners before and after using these parameters. In Part I, 12 specialists analyzed 24 images and the diagnostic criteria used by each examiner to make correct diagnoses were used to build a list of basic radiographic parameters for each pathology (ameloblastoma, keratocystic odontogenic tumor, dentigerous cyst, and idiopathic bone cavity). In Part II, this list was used by 6 undergraduate students (Un), 8 recently graduated dentists (D), 3 oral pathologists, 3 stomatologists, 3 oral radiologists, and 3 oral surgeons to diagnose the corresponding pathologies in the other set of 24 panoramic radiographs (T2). The same analysis occurred without using this list (T1). The method of generalized estimating equations (GEE) was used in order to estimate the probability of making a correct diagnosis depending on the specialty of the examiner, type of lesion, and moment of the evaluation, T1 or T2 (before or after they had access to the list of parameters, respectively). Higher values were obtained for the probability (GEE) of making a correct diagnosis on T2; the group Un presented the highest improvement (14.6 %); no differences between the probabilities were observed either between Un and D, or among the different groups of specialists. The use of panoramic radiographic parameters did allow improving the diagnostic accuracy for all groups of examiners.

  16. Odontogenic lesions in pediatric patients.

    Science.gov (United States)

    Fang, Qi-Gen; Shi, Shuang; Sun, Chang-Fu

    2014-05-01

    The purpose was to evaluate our 20-year experience of pediatric odontogenic lesions. Pediatric patients with a diagnosis of odontogenic lesion were identified. Three hundred ten patients were odontogenic; dentigerous cyst was seen in 62.0% of the cases. Most (70.2%) of them occurred in mixed dentition period, and it had a male preponderance. Odontogenic keratocystic tumor occurred in the permanent dentition period. It had an equal site distribution. Odontoma was seen in 20.0% of the cases. Its site of predilection was the mandible. Ameloblastoma was the most common odontogenic tumor. Most of the cases occurred in the permanent dentition period. It affected the male and female equally. Calcifying epithelioma odontogenic tumor was seen in 11.8% of the cases. All the lesions occurred in the primary dentition period. It had no sex or site preponderance. Myxoma was seen in 3.6% of the cases. It was most common in the permanent dentition period, and it was more frequent in the male. Iliac crest bone graft was successfully performed in 28 patients, postoperative infection occurred in 2 patients, and no donor-site dysfunctions were reported. The observed differences in lesion type and distribution in this study compared with previous researches may be attributable to genetic and geographic variation in the populations studied. Iliac crest bone graft was suggested for pediatric mandible reconstruction.

  17. Effectiveness of surgical decompression in the treatment of a calcifying cystic odontogenic tumor

    Directory of Open Access Journals (Sweden)

    Yuri Slusarenko da Silva

    2014-12-01

    Full Text Available The calcifying odontogenic cystic tumor (CCOT is a benign lesion of odontogenic origin characterized by an ameloblastoma-like epithelium with ghost cells that may calcify. Despite broadly considered as a cyst, some investigators prefer to classify it as a neoplasm. Clinically, it occurs predominantly during the third decade of life. No difference in gender prevalence has been observed nor predilection of the lesion between maxilla and mandible. The most affected region extends from the incisor tooth to bicuspids. The classic treatment of the lesion is full excision, although a different approach may be determined by the possible association with another odontogenic tumor. Depending on the tumor size and the vicinity with important structures, decompression may be undertaken before its complete removal. The present report describes a case of CCOT with large proportions, located at the right maxilla and extending to the maxillary sinus, nasal cavity, and orbital floor. The treatment option was surgical decompression as the initial procedure, with satisfactory outcome. After partial remission, the lesion was fully removed, and the post-operative follow-up was uneventful.

  18. Histopathologic changes in soft tissue associated with radiographically normal impacted third molars

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    Kotrashetti Vijayalakshmi

    2010-01-01

    Full Text Available Background: The incidence of impacted or embedded third molars accounts for approximately 98%. Since 1948, there are studies reporting pathological changes in an asymptomatic dental follicle. Controversy still exists for removal of asmptomatic impacted teeth. Hence, this study was performed to histologically evaluate soft tissue pathosis in the pericoronal tissues of impacted third molars with pericoronal radiolucency measuring up to 2.5 mm on orthopantomographs. Materials and Methods: Forty-one asymptomatic impacted third molars with follicular space of up to 2.5 mm on radiographs were included. The disimpacted teeth and the follicular tissues were obtained for histological examination. Results: Age of the patients ranged from 14 to 25 years. Of 41 tissues evaluated, histopathological reports of 18 follicles were suggestive of dentigerous cyst, two follicles showed odontogenic keratocyst, one follicle each of calcifying epithelial odontogenic cyst, ameloblastoma-like proliferation, odontogenic myxoma and odontogenic fibroma. Conclusion: This study showed 58.5% of asymptomatic cases with definite pathological changes. Hence, thorough clinical and radiographic examination should be carried out for all impacted third molars and the dental follicular tissue should be submitted for histopathological evaluation.

  19. Occurrence of bone cancer among young adult Beagles given 239Pu

    International Nuclear Information System (INIS)

    Lloyd, R.D.; Taylor, G.N.; Bruenger, F.W.; Angus, W.; Miller, S.C.

    1991-01-01

    Two hundred thirty-five young adult Beagles of both sexes were each given a single intravenous injection of 239 Pu-citrate at graded dose-levels averaging about 0.026 to 106 kBq/kg when they were about 1 1/2 years of age and were maintained for lifespan observation. An additional 133 young adult Beagles of both sexes were entered into the experiment as control animals. All of these animals have now died or have been removed from the colony, and the occurrence of skeletal malignancies has been determined from histological examination. There were a total of 85 radiographically apparent malignant bone tumors in 77 dogs given 239 Pu, and there was one control animal that developed a skeletal malignancy. Most of these were osteosarcomas, but there were seven chondrosarcomas of bone, one liposarcoma of bone, and in addition, there was one plasma cell myeloma and one ameloblastoma (admantinoma). Only those dogs that survived to at least the minimum latent period for death with radiation-induced bone sarcoma are included in the tabulation. There appeared to be a linear relationship between the percent of dogs with bone tumor and the average skeletal dose up to a dose of about 1 Gy. All dose-levels with skeletal doses of about 2 Gy and greater exhibited close to 100% occurrence

  20. Immunohistochemical expression of GLUT-1, GLUT-3, and carbonic anhydrase IX in benign odontogenic lesions.

    Science.gov (United States)

    Vasconcelos, Roseane Carvalho; de Oliveira Moura, Jamile Marinho Bezerra; Lacerda Brasileiro Junior, Vilson; da Silveira, Éricka Janine Dantas; de Souza, Lélia Batista

    2016-10-01

    Some benign odontogenic lesions have a distinct biological behavior with high recurrence rates and local aggressive behavior. To determine whether glucose transporters proteins (GLUT-1 and GLUT-3) and carbonic anhydrase IX (CA IX) are associated with the development of as dentigerous cyst (DC), odontogenic keratocyst (OK), and ameloblastoma (AM), we evaluated the immunohistochemical expression of these proteins in these lesions. Immunoexpression of GLUT-1, GLUT-3, and CA IX was evaluated semiquantitative fields in each of the 20 cases of OK, AM, and DC. The cases were classified according to the scores: 0 (0% positive cells), 1 (50% of positive cells). The statistical analysis was performed using Pearson's chi-square, Kruskal-Wallis and Mann-Whitney tests. All cases were positive for GLUT-1 and 65% of OK showed scored 3. Staining was diffuse in 90% of OK and 85% of DC cases (P GLUT-3. Staining intensity for anhydrase was higher in the epithelium of DC when compared to OK (P = 0.01). Strong staining was observed in 55% of DC and 20% of OK samples (P = 0.01). These results suggest that GLUT-1 may be involved in the metabolic regulation of glucose in odontogenic lesions studied. In addition, CA IX appears to influence the development of AM, OK, and DC which can explain the differences their biological behavior. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  1. Palliative radiation therapy for solid tumors in dogs: 103 cases (2007-2011).

    Science.gov (United States)

    Tollett, Melissa A; Duda, Lili; Brown, Dorothy C; Krick, Erika L

    2016-01-01

    To evaluate the clinical response, adverse effects, and outcomes associated with palliative radiation therapy (PRT) in dogs with various solid tumor types at various body locations. Retrospective case series. 103 dogs with solid tumors. Medical records for dogs with solid tumors treated with PRT between July 2007 and January 2011 at a veterinary teaching hospital were reviewed. Data collected included signalment, tumor type and location, initial staging results, PRT protocol, other tumor-specific treatments, patient and tumor response, outcome, and acute and chronic adverse effects. Median progression-free survival time, median survival time (MST), and other descriptive statistics were calculated. Types of tumors treated included carcinoma, sarcoma, melanoma, primary bone tumor, mast cell tumor, and ameloblastoma. For all dogs, the overall tumor and clinical response rates to PRT were 75% and 77%, respectively, and the MST was 134 days, but those responses varied substantially among tumor types. Dogs that developed a positive clinical response or maintained stable disease after PRT had a significantly longer MST than did dogs with progressive disease. Tumor location was not significantly associated with median progression-free survival time or MST. Most dogs tolerated the PRT well. Acute and chronic adverse effects were observed in 57 and 8 dogs, respectively, but were generally self-limiting. Results indicated that dogs with various types of solid tumors that received PRT had objective beneficial responses and an improvement in quality of life that was positively associated with survival time.

  2. Mandibular metastasis of rectum adenocarcinoma: case report

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    Mehmet Ali Çetin

    2018-01-01

    Full Text Available Introduction: Mandibular metastases are quite rare and they constitute less than about %1 of the mandibular malignancies. In mandibular metastatic cases, patients’ complaints generally include bony swelling with pain, tooth pain and tooth loss. Mandibular metastatic lesions mimic periodontal diseases; therefore careful examination is necessary. In differential diagnosis, osteosarcoma, which is a primary bone tumor, primary intraosseous squamous carcinoma, ameloblastoma, and temporomandibular joint diseases should be taken into consideration. Treatment modalities are surgery and chemoradiotherapy. Because of the poor prognosis, the goal of the treatment is only palliative. Case Report: A 51-year-old woman, operated due to rectal adenocarcinoma 3 years previously, was referred to our clinic with a complaint of swelling on the jaw. Cranial magnetic resonance imaging and maxillofacial computed tomography revealed a mass that extended to condyle and lead to bone destruction on the right ramus of the mandible. An incisional biopsy was performed. The histopathology was consistent with colorectal adenocarcinoma metastasis. The patient was referred to a medical oncologist. Conclusion: Differential diagnosis of mandibular lesions should be made carefully, and metastatic tumors should be kept in mind. In patients with history of lung cancer, breast cancer, colorectal carcinoma or similar tumors, oral findings such as tooth pain and tooth loss should be taken into consideration, as these may be the signs of head and neck metastases. Biopsy must be performed after radiological examination.

  3. Allogenic lyophilized cartilage grafts for craniomaxillofacial reconstruction

    International Nuclear Information System (INIS)

    Pill Hoon Choung

    1999-01-01

    Allogenic lyophilized cartilages were made in our clinic after Sailer methods and some modification. In our clinic, we have used allogenic cartilage grafts on 102 defects of craniomaxillofacial area; 1) for defects from cyst or ameloblastoma, 2) for lack of continuity of the mandible, 3) for rhinoplasty, 4) for paranasal augmentation, 5) for augmentation genioplasty, 6) for reconstruction of orbital floor, 7) for oroantral fistula, 8) for temporal augmentation, 9) for TMJ surgery 10) for condyle defect as a costochondral graft, 11) for filling of tooth socket and alveolus augmentation,12) for correction or orbital height and 13) for guided bone regeneration in peripheral implant. The types of lyophilized cartilage used were chip, sheet and block types developed by freeze-dried methods. Some grafts showed change of ossification, in which case we could perform implant on it. We have good results on reconstruction of craniomaxillofacial defects. Allogenic cartilage have advantages such as 1) it has no immune reaction clinically, 2) it is more tolerable to infection than that of autogenous cartilage, 3) it has character of less resorption which require no over correction, 4) it is easy to manipulate contouring, and 5) it has possibility of undergoing ossification. Allogenic cartilage has been considered as good substitutes for bone. The author would like to report the results on 102 allogenic cartilage have

  4. Low-cost Method for Obtaining Medical Rapid Prototyping Using Desktop 3D printing: A Novel Technique for Mandibular Reconstruction Planning.

    Science.gov (United States)

    Velasco, Ignacio; Vahdani, Soheil; Ramos, Hector

    2017-09-01

    Three-dimensional (3D) printing is relatively a new technology with clinical applications, which enable us to create rapid accurate prototype of the selected anatomic region, making it possible to plan complex surgery and pre-bend hardware for individual surgical cases. This study aimed to express our experience with the use of medical rapid prototype (MRP) of the maxillofacial region created by desktop 3D printer and its application in maxillofacial reconstructive surgeries. Three patients with benign mandible tumors were included in this study after obtaining informed consent. All patient's maxillofacial CT scan data was processed by segmentation and isolation software and mandible MRP was printed using our desktop 3D printer. These models were used for preoperative surgical planning and prebending of the reconstruction plate. MRP created by desktop 3D printer is a cost-efficient, quick and easily produced appliance for the planning of reconstructive surgery. It can contribute in patient orientation and helping them in a better understanding of their condition and proposed surgical treatment. It helps surgeons for pre-operative planning in the resection or reconstruction cases and represent an excellent tool in academic setting for residents training. The pre-bended reconstruction plate based on MRP, resulted in decreased surgery time, cost and anesthesia risks on the patients. Key words: 3D printing, medical modeling, rapid prototype, mandibular reconstruction, ameloblastoma.

  5. Mandibular reconstruction using stereolithographic 3-dimensional printing modeling technology.

    Science.gov (United States)

    Cohen, Adir; Laviv, Amir; Berman, Phillip; Nashef, Rizan; Abu-Tair, Jawad

    2009-11-01

    Mandibular reconstruction can be challenging for the surgeon wishing to restore its unique geometry. Reconstruction can be achieved with titanium bone plates followed by autogenous bone grafting. Incorporation of the bone graft into the mandible provides continuity and strength required for proper esthetics and function and permitting dental implant rehabilitation at a later stage. Precious time in the operating room is invested in plate contouring to reconstruct the mandible. Rapid prototyping technologies can construct physical models from computer-aided design via 3-dimensional (3D) printers. A prefabricated 3D model is achieved, which assists in accurate contouring of plates and/or planning of bone graft harvest geometry before surgery. The 2 most commonly used rapid prototyping technologies are stereolithography and 3D printing (3DP). Three-dimensional printing is advantageous to stereolithography for better accuracy, quicker printing time, and lower cost. We present 3 clinical cases based on 3DP modeling technology. Models were fabricated before the resection of mandibular ameloblastoma and were used to prepare bridging plates before the first stage of reconstruction. In 1 case, another model was fabricated and used as a template for iliac crest bone graft in the second stage of reconstruction. The 3DP technology provided a precise, fast, and cheap mandibular reconstruction, which aids in shortened operation time (and therefore decreased exposure time to general anesthesia, decreased blood loss, and shorter wound exposure time) and easier surgical procedure.

  6. Post-operative re-construction of dentoalveolar tissue and the mandible and maxillae using prosthetic therapy.

    Science.gov (United States)

    Mutave, R; Chindia, M L; Guthua, S W

    2005-06-01

    To determine the range of ablative surgery and rehabilitative procedures performed on maxillofacial structures. A retrospective descriptive study. University of Nairobi Dental Teaching Hospital. Patients operated on at the institutions theatre, and followed up at the University of Nairobi Dental Teaching Hospital Oral Surgery Outpatient Clinic during the period February 1996, August 1998. Forty four patients underwent ablative surgery during the study period. Complete records were available for 38 patients, 27 females and 11 males aged 10 to 79 years (mean 32.6 years). Surgical procedures performed included: partial mandibulectomy, marginal mandibular resection, subtotal maxillectomy, sequestrectomy and enucleation. Six (15.8%) patients who underwent mandibular resection had rigid bone plating. Five (13.2%) patients who had maxillary involvement were given surgical obturators post-operatively with one receiving a complete denture after full recovery. A total of 22 (57.9%) patients who would have reaped benefits from prostheses therapy received none. Individual patient follow-up periods ranged from seven days for two patients who had cyst enucleation to two years for three cases with ameloblastoma, and two cases with squamous cell carcinoma. It is concluded that prosthetic rehabilitation of patients undergoing extensive surgery at the University of Nairobi Dental Teaching Hospital is largely inadequate.

  7. AVALIAÇÃO EPIDEMIOLÓGICA DE CÃES COM NEOPLASIAS ORAIS ATENDIDOS NO HOSPITAL DE CLÍNICAS VETERINÁRIAS DA UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL EPIDEMIOLOGICAL EVALUATION OF THE DOGS WITH ORAL TUMORS ATTENDED IN THE VETERINARY HOSPITAL OF FEDERAL UNIVERSITY OF RIO GRANDE DO SUL

    Directory of Open Access Journals (Sweden)

    Ruben Cavalcanti

    2009-09-01

    Full Text Available Os tumores orais em cães representam cerca de 6% de todas as neoplasias dessa espécie. O objetivo deste trabalho foi realizar um estudo epidemiológico dos cães com tumores orais atendidos no Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul durante o período de julho de 2003 a julho de 2007. Realizou-se a pesquisa nos arquivos do Laboratório de Histopatologia e nas fichas clínicas dos animais, para avaliação de dados como raça, sexo, idade e o tipo histológico dos tumores. Procedeu-se à análise de 79 cães, dentre os quais 39 (49,37% eram machos e 40 (50,63% eram fêmeas, com idades variando entre um e dezesseis anos (média de 7,7 anos. Os cães mais acometidos foram os sem raça definida, com dezesseis casos (19,76%, seguido pela raça Poodle, com doze casos (14,82%. As neoplasias malignas representaram 50,63% dos casos e as benignas 49,37%%. Os tumores mais comumente encontrados foram o melanoma e o épulis acantomatoso, com dezenove casos cada (23,46%, seguidos pelo fibrossarcoma e o épulis fibromatoso, com nove casos cada (11,11%, plasmocitoma com oito casos (9,88%, ameloblastoma com quatro casos (4,94%, carcinoma epidermoide e osteossarcoma com três casos cada (3,7%. Por meio deste estudo, verificou-se que os tumores malignos apresentam uma maior incidência, e os tipos histológicos mais frequentes foram o melanoma, o fibrossarcoma, o épulis acantomatoso e fibromatoso.
     
    PALAVRAS-CHAVES: Câncer, cães, tumores orais. Oral tumors reach about 6% of all the neoplasm in dogs. The objective of this research was to form an epidemiological study on dogs with oral tumors that have been treated at the Veterinary Hospital of Federal University of rio Grande do Sul from July 2003 to July 2007. The research was conducted within the archives of the histopathology laboratory, using the animals’ clinical files, in which they had been categorized by breed, sex, age and the tumors

  8. Is podoplanin expression associated with transforming growth factor-β signaling in odontogenic cysts and tumors?

    Science.gov (United States)

    Etemad-Moghadam, Shahroo; Alaeddini, Mojgan

    2018-03-26

    Induction of podoplanin by transforming growth factor-β (TGF-β) has been shown in a number of lesions but not in odontogenic tumors (OTs). We evaluated the association between these markers in OTs for the first time and compared their expression among the different neoplasms. Immunohistochemistry using monoclonal antibody against podoplanin and TGF-β was performed on 76 odontogenic cysts and tumors. Spearman's correlation coefficient, Kruskal-Wallis, and Mann-Whitney U tests followed by adjustment with Bonferroni were used for statistical analysis (P < .05). A significant difference in podoplanin expression was found among the lesions consisting of solid ameloblastomas, adenomatoid odontogenic tumors, ameloblastic fibromas, odontogenic myxomas (OMs), odontogenic keratocysts, and calcifying odontogenic cysts. Significant differences were observed only between OMs and each of the other neoplasms. Podoplanin immunostaining in the connective tissue was absent in most lesions. TGF-β was significantly different among the study sample but not between the lesions in paired comparisons. None of the studied OTs showed significant correlations between podoplanin-TGF-β, in either the epithelium or the stroma. These markers were also descriptively reported in calcifying epithelial odontogenic tumors. The inductive effect of TGF-β on podoplanin seems to be limited, if any, in odontogenic lesions. Podoplanin appears to play a role in some aspects of OTs with epithelial or mixed origins. Despite the possible participation of podoplanin in tumorigenesis, it may not necessarily be involved in the aggressive behavior of OTs. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  9. Audit of Oral Histopathology Service at a Nigerian Tertiary Institution over a 24-Year Period.

    Science.gov (United States)

    Akinyamoju, Akindayo O; Adeyemi, Bukola F; Adisa, Akinyele O; Okoli, Chidinma N

    2017-07-01

    Biopsies are often essential for definitive diagnosis of oro-facial lesions and are a part of oral diagnostic procedures carried out in histopathology laboratories. At present, there is paucity of literature on the audit of oral histopathology services in Nigeria. The objectives of this study were to determine the prevalence of biopsied oral lesions in a Nigerian tertiary institution. Also to profile the usage of oral pathology service and to identify challenges that may be present in an oral histodiagnostic service. This was a retrospective study performed at the Oral Pathology Department of the University of Ibadan/ University College Hospital, Ibadan, Nigeria. Reports of all biopsies submitted at the Oral Pathology laboratory, for the period 1990-2014, were reviewed and data extracted. Descriptive analysis was done using SPSS software, version 20. The total number of reports was 1,998; invalid reports constituting 220(11%) were subsequently excluded leaving 1,778(89%) valid reports. The mean age of patients was 36.70±19.79, while the peak age of presentation was in the 3 rd decade. Male to female ratio was 1:1.1, and the mandible was the most common site of lesions 619(34.8%). These services were mainly utilized by oral surgeons (83.9%) and ameloblastoma (11.5%) was the most frequently diagnosed lesion. CD45 (16.7%) was the most frequently requested immuno-diagnostic test. Biopsied oral lesions were more prevalent in females, while oral and maxillofacial surgeons utilized these services the most. Inadequate biopsy specimens or unrepresentative specimens and deficient documentation were challenges identified in this study.

  10. Diagnostic role and limitations of FNAC in oral and jaw swellings.

    Science.gov (United States)

    Goyal, Surbhi; Sharma, Sonal; Diwaker, Preeti

    2015-10-01

    Fine-needle aspiration cytology (FNAC) of oral lesions has not been widely utilized for diagnosis due to rarity and diversity of lesions, peculiar anatomy of maxillofacial region, difficulty in aspirating these lesions, and limited experience. Aim of this study was to determine the role of FNAC in the diagnosis of oral and jaw swellings. One hundred and forty two patients underwent FNAC over a period of 7 years (2007-2013), of which 127 (89.4%) aspirates were diagnostic. Histopathologic correlation was available in 83 cases and diagnostic accuracy of FNAC was calculated. Of the minor salivary gland lesions, 41 out of 55 lesions (74.5%) were benign and 14 (25.4%) were malignant. Mucocele was the most common lesion in oral cavity (30 cases) and pleomorphic adenoma was the commonest salivary gland neoplasm (9 cases). Of the 72 non-salivary-gland lesions, 22 lesions were inflammatory, 4 were epidermal inclusion cysts, 21 were neoplastic, and the remaining 25 presented as radiolucent jaw bone lesions. Diagnostic accuracy of FNAC in our study was 91.6% with 6 false negatives and 1 false positive. Four glandular malignancies and one case of cystic ameloblastoma were misdiagnosed as false negative on cytology. Cytological features are diagnostic in certain inflammatory lesions of infective etiology and neoplastic conditions like Langerhans cell histiocytosis, lymphoma, plasmacytoma, Ewing's tumor, and squamous cell carcinoma. However, accurate subtyping of giant cell lesions, salivary gland tumors, odontogenic tumors, and cystic lesions may not be always feasible on FNAC. FNAC is highly accurate in early diagnosis of oral and jaw lesions. © 2015 Wiley Periodicals, Inc.

  11. Jaw Intraosseous Lesions Biopsied Extracted From 1998 to 2010 in an Iranian Population.

    Science.gov (United States)

    Jamshidi, Shokoofeh; Shojaei, Setareh; Roshanaei, Ghodratollah; Modabbernia, Shirin; Bakhtiary, Esmaeel

    2015-06-01

    Jaw bones might be potential locations for different lesions. Differences in prevalence and the type of lesions can help in designing and programming prevention procedures in health care centers. The aim of the present study was to evaluate the prevalence of intraosseous lesions in the jaws of patients referred to diagnostic and therapeutic centers in Hamadan during 1990-2010. This cross-sectional descriptive analytical study was carried out in Hamadan in 2011. Data sheets of the subjects were used to collect all the data of patients with intraosseous lesions, including their age, gender, location of the lesion, the radiographic view of lesions, and their type and histopathological diagnoses. Data were analyzed with SPSS, using means and frequencies. A total of 284 intraosseous lesions were reported in our study. The mean age of the subjects was 28.8 ± 15.2 years. The lesions were distributed in males and females almost similarly. The most prevalent lesions were cystic lesions (54.58%), manifestations of systemic conditions in jaw bones (18.3%), benign tumors (15.5%), malignant lesions (6.7%), and inflammatory lesions (4.92%), in a descending order. The most common cystic lesion was radicular cyst; the most common manifestation of systemic conditions in jaw bones was central giant cell granuloma; the most common benign tumor was ameloblastoma; the most common malignant lesion was osteosarcoma; and the most common inflammatory lesion was periapical granuloma. Our data provided information on the prevalence and types of intraosseous lesions among an Iranian population. This study provided baseline information to help in designing and programming procedures in health care centers in every community so that preventive therapeutic measures can be adopted.

  12. Novel PTCH1 mutations in patients with keratocystic odontogenic tumors screened for nevoid basal cell carcinoma (NBCC syndrome.

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    Lorenza Pastorino

    Full Text Available Keratocystic odontogenic tumors (KCOTs are cystic tumors that arise sporadically or associated with nevoid basal cell carcinoma syndrome (NBCCS. NBCCS is a rare autosomal dominantly inherited disease mainly characterized by multiple basal cell carcinomas, KCOTs of the jaws and a variety of other tumors. PTCH1 mutation can be found both in sporadic or NBCCS associated KCOTs. The aim of the current study was to assess whether a combined clinical and bio-molecular approach could be suitable for the detection of NBCCS among patients with a diagnosis of keratocystic odontogenic tumors (KCOTs. The authors collected keratocystic odontogenic tumors recorded in the database of the Pathology Department of the University of Modena and Reggio Emilia during the period 1991-2011. Through interviews and examinations, family pedigrees were drawn for all patients affected by these odontogenic lesions. We found out that 18 of the 70 patients with KCOTs and/or multiple basal cell carcinomas actually met the clinical criteria for the diagnosis of NBCCS. A wide inter- and intra-familial phenotypic variability was evident in the families. Ameloblastomas (AMLs were reported in two probands that are also carriers of the PCTH1 germline mutations. Nine germline mutations in the PTCH1 gene, 5 of them novel, were evident in 14 tested probands. The clinical evaluation of the keratocystic odontogenic tumors can be used as screening for the detection of families at risk of NBCCS. Keratocystic odontogenic lesions are uncommon, and their discovery deserves the search for associated cutaneous basal cell carcinomas and other benign and malignant tumors related to NBCCS.

  13. Jaw expansive lesions: population incidence and CT dentalscan role.

    Science.gov (United States)

    Fiaschetti, V; Fanucci, E; Rascioni, M; Ottria, L; Barlattani, A; Simonetti, G

    2010-07-01

    The aim of the study is to evaluate the incidence of different expansive lesions and the advantages of the clinical employment of Dentalscan to study bones lesions and to establish a common diagnostic path. Since January 2005 to November 2009, 3200 patients, not selected for sex or age, have undergone a CT "Dentalscan" in the department of Diagnostic Imaging, Tor Vergata University Hospital (PTV), a suspect bone pathology was found in 704 of them through the XR-orthopantomograpy (OPT). CT images were obtained with General Electric CT Light Speed multislice. Images were saved in the Advantage Workstation (GE) supported by the "Dentascan" dedicated software and by the 3D software (3D SSD). The protocol was : Slice thickness 1,25 mm, gap 0, matrix 512 × 512, 140 KV and 70 mA. All the lesions were also studied with the dedicated three-dimensional reconstructor 3D SSD. Biopsy for diagnosis was performed on all the lesions, except one (false positive with digital OPT). THE TECHNIQUE SENSITIVITY WAS ASSESSED FOR TWO IMPORTANT CLASSES: benign and malignant lesions. Through CT Dentascan a detailed evaluation of the jaws lesions and their extension was obtained. 656 patients (93.1 %) out of 704 examined for a suspicious lesion on the orthopantomography had a benign lesion: (127 follicular cysts (18.2 %), 181 radicular cysts (25.1%), 93 non odontogenic cysts (13.2%), 29 fibroma (4.2%), 198 odontomes (28.2%), 24 ameloblastoma (3.6%), 4 brown tumors (0.7%), 47 (6.9%) had malignant lesions: (12 carcinoma (1.7%), 29 metastasis (4.3%), 6 sarcoma (0.8%), 1 Dentascan CT resulted to be negative (1 false positive of digital OPT). The sensitivity of the technique for both groups was 99% for benign lesions and 98% for malign lesions. CT Dentascan characteristics suggest to consider these techniques as the gold standard for the evaluation of jaw expansive lesions and the support of surgical planning.

  14. Adipose stem cell tissue-engineered construct used to treat large anterior mandibular defect: a case report and review of the clinical application of good manufacturing practice-level adipose stem cells for bone regeneration.

    Science.gov (United States)

    Sándor, George K; Tuovinen, Veikko J; Wolff, Jan; Patrikoski, Mimmi; Jokinen, Jari; Nieminen, Elina; Mannerström, Bettina; Lappalainen, Olli-Pekka; Seppänen, Riitta; Miettinen, Susanna

    2013-05-01

    Large mandibular resection defects historically have been treated using autogenous bone grafts and reconstruction plates. However, a major drawback of large autogenous bone grafts is donor-site morbidity. This report describes the replacement of a 10-cm anterior mandibular ameloblastoma resection defect, reproducing the original anatomy of the chin, using a tissue-engineered construct consisting of β-tricalcium phosphate (β-TCP) granules, recombinant human bone morphogenetic protein-2 (BMP-2), and Good Manufacturing Practice-level autologous adipose stem cells (ASCs). Unlike prior reports, 1-step in situ bone formation was used without the need for an ectopic bone-formation step. The reconstructed defect was rehabilitated with a dental implant-supported overdenture. An additive manufactured medical skull model was used preoperatively to guide the prebending of patient-specific hardware, including a reconstruction plate and titanium mesh. A subcutaneous adipose tissue sample was harvested from the anterior abdominal wall of the patient before resection and simultaneous reconstruction of the parasymphysis. ASCs were isolated and expanded ex vivo over the next 3 weeks. The cell surface marker expression profile of ASCs was similar to previously reported results and ASCs were analyzed for osteogenic differentiation potential in vitro. The expanded cells were seeded onto a scaffold consisting of β-TCP and BMP-2 and the cell viability was evaluated. The construct was implanted into the parasymphyseal defect. Ten months after reconstruction, dental implants were inserted into the grafted site, allowing harvesting of bone cores. Histologic examination and in vitro analysis of cell viability and cell surface markers were performed and prosthodontic rehabilitation was completed. ASCs in combination with β-TCP and BMP-2 offer a promising construct for the treatment of large, challenging mandibular defects without the need for ectopic bone formation and allowing

  15. Histopathological and Radiographic Analysis of Dental Follicle of Impacted Teeth Using Modified Gallego’s Stain

    Science.gov (United States)

    Satheesan, Evie; Tamgadge, Avinash; Bhalerao, Sudhir; Periera, Treville

    2016-01-01

    Introduction In the WHO classification of odontogenic tumours, hard tissue formation has been considered as a sub-classification however, this parameter has not been much explored in dental follicle in literature. Epithelial-mesenchymal interactions play an important role in odontogenesis and its associated pathologies; therefore research on dental follicle should also include mesenchymal components along with epithelial components. Additionally, special stains to identify the nature of such depositions in dental follicle have been less explored. Modified Gallego’s stain is such an example which has not been tried in odontogenic lesions which makes this study unique. Aim Aim of this study was to study histopathological variations in dental follicle, the nature of calcification and depositions using Modified Gallego’s stain and to correlate histological features of dental follicle with pericoronal width radiographically. Materials and Methods A prospective histological study of the dental follicles of 50 impacted teeth was carried out to microscopically evaluate the dental follicular tissues for pathological changes, and to correlate it with pericoronal radiolucency. Impacted teeth with pericoronal radiographic width less than 3mm were included in the study and symptomatic teeth were excluded. Further Modified Gallego stain was used to differentiate the nature of hard tissue formation in dental follicle tissues. Results Dental follicle histologically showed pathological changes resembling dentigerous cyst, ameloblastoma, odontogenic fibroma (Simple and WHO Type), clear cell odontogenic tumour, neurofibroma, neurilemmoma and mucoepidermoid carcinoma. Conclusion The dental follicle surrounding an impacted tooth has the potential to differentiate into a wide variety of tissue types, and thus shows the potential for cyst and tumour development which was observed in this study in most of the specimens with normal follicular width radiographically. PMID:27437341

  16. Spontaneous Peripheral Ameloblastic Odontoma in a Male Sprague-Dawley Rat.

    Science.gov (United States)

    Li, Yinghua; Bae, Han-Ik; Kim, Hak-Soo; Kang, Min-Soo; Gong, Bo-Ho; Jung, Won-Hee; Lee, Sranna; Bae, Jin-Sook; Kim, Kap-Ho; Song, Si-Whan; Lee, Jae-Hyun; Kang, Boo-Hyon

    2017-04-01

    Peripheral ameloblastic odontoma is a rare variant of odontogenic tumor occurring in the extraosseous region. The present report describes a spontaneous tumor in male Sprague-Dawley (SD) rats. The clinically confirmed nodule in the right mandibular region was first observed when the rat was 42 weeks and remained until the terminal sacrifice date when the animal was 48 weeks of age. At necropsy, a well demarcated nodule, approximately 2.5 × 2.0 × 2.0 cm, protruded from the ventral area of the right mandible. The nodule was not attached to mandibular bone and was not continuous with the normal teeth. Histopathologically, the tumor was characterized by the simultaneous occurrence of an ameloblastomatous component and composite odontoma-like elements within the same tumor. The epithelial portion formed islands or cords resembling the follicle or plexiform pattern typical of ameloblastoma and was surrounded by mesenchymal tissue. Formation of eosinophilic and basophilic hard tissue matrix (dentin and enamel) resembling odontoma was observed in the center of the tumor. Mitotic figures were rare, and areas of cystic degeneration were present. Immunohistochemically, the epithelial component was positive for cytokeratin AE1/AE3 (CK AE1/AE3), and the mesenchymal component and odontoblast-like cells were positive for vimentin, in the same manner as in normal teeth. On the basis of these findings, the tumor was diagnosed as a peripheral ameloblastic odontoma in an extraosseous mandibular region in a SD rat. In the present study, we report the uncommon spontaneous peripheral ameloblastic odontoma in the SD rat. We also discuss here the morphological characteristics, origin, histochemical, and immunohistochemical features for the diagnosis of this tumor.

  17. Primordial Odontogenic Cyst with Induction Phenomenon (Zonal Fibroblastic Hypercellularity) and Dentinoid Material Versus Archegonous Cystic Odontoma: You Choose!

    Science.gov (United States)

    Argyris, Prokopios P; Wetzel, Stephanie L; Pambuccian, Stefan E; Gopalakrishnan, Rajaram; Koutlas, Ioannis G

    2016-06-01

    The most recent A.F.I.P. fascicle defines primordial odontogenic cyst (POC) as a distinct, nonkeratinized, odontogenic cyst of "undetermined origin" forming in the place of a developing normal or supernumerary tooth. However, the majority of examples reported in the literature under this term represent odontogenic keratocysts (keratocystic odontogenic tumors). In addition, there are rare reported cases of cystic odontomas. An 18-year-old Caucasian male presented with a unilocular mandibular radiolucent lesion in the place of a congenitally missing molar. Histologically, it featured nonkeratinizing, thin stratified squamous epithelial lining with areas of spongiosis and foci of vacuolization of individual basal cells without significant nuclear palisading. Focally, budding of the basal cell layer was identified. A zone of increased cellularity featuring induction-type fibroblasts was present subepithelially as well as dentinoid deposits with odontogenic epithelial nests. Immunohistochemically, the epithelial lining was negative for calretinin and the induction-like zone negative for S100 protein, smooth muscle actin, and CD34. The case was externally reviewed by five oral pathologists who provided various diagnostic interpretations including primordial cyst, odontogenic cyst not otherwise specified (NOS), cyst with ameloblastic changes, and unicystic ameloblastoma. At that time, a final diagnosis of odontogenic cyst NOS was rendered with a comment that it may represent a true example of POC or a cystic odontoma. The lesion has not recurred within a 13 year follow-up period after initial excision. An unusual cystic lesion is presented that may represent a true example of POC with dentinoid formation or an archegonous cystic odontoma.

  18. Intraepithelial expression of perlecan, a basement membrane-type heparan sulfate proteoglycan reflects dysplastic changes of the oral mucosal epithelium.

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    Ikarashi, Terué; Ida-Yonemochi, Hiroko; Ohshiro, Kazufumi; Cheng, Jun; Saku, Takashi

    2004-02-01

    Intercellular deposition of perlecan, a major heparan sulfate proteoglycan (HSPG) of the basement membrane, is known to result in characteristic stellate reticulum-like structures in ameloblastomas or tooth germs. Although enlargement of the intercellular space is one of the histological characteristics of epithelial dysplasia of oral mucosa, the mode of expression of perlecan is poorly understood in these epithelial lesions. Eighty-two biopsy specimens consisting of normal and hyperplastic epithelium, epithelial dysplasia, and squamous cell carcinomas were examined for both perlecan core protein and heparan sulfate (HS) chains by immunohistochemistry and in situ hybridization. In normal and hyperplastic epithelium, perlecan core protein and HS chains were localized in the cell border of parabasal cells and lower prickle cells, and HS chains were also found in basal cells. With an increase in the severity of epithelial dysplasia, the core protein was heavily and extensively deposited in the interepithelial space as well as in the cytoplasm of epithelial cells from the basal to the surface layers. Its gene expression was confirmed in the cells around the protein deposits. On the other hand, HS chains were enhanced in mild dysplasia, but decreased in moderate and severe dysplasias. In squamous cell carcinomas, either the core protein or HS chains were found scarcely in tumor cells but abundantly in the stromal space. The findings indicate that perlecan is localized in the intercellular space of the oral epithelia, and that it is over-expressed in dysplastic epithelial cells and is deposited in their interepithelial space, which results in the histology of reduction of cellular cohesion.

  19. Success of dental implants in vascularised fibular osteoseptocutaneous flaps used as onlay grafts after marginal mandibulectomy.

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    Chang, Y-M; Pan, Y-H; Shen, Y-F; Chen, J-K; ALDeek, N F; Wei, F-C

    2016-12-01

    We have evaluated the survival of dental implants placed in vascularised fibular flap onlay grafts placed over marginal mandibulectomies and the effects on marginal bone loss of different types of soft tissue around implants under functional loading. From 2001-2009 we studied a total of 11 patients (1 woman and10 men), three of whom had had ameloblastoma and eight who had had squamous cell carcinomas resected. A total of 38 dental implants were placed either at the time of transfer of the vascularised fibular ostoseptocutaneous flaps (nine patients with 30 implants) or secondarily (two patients with eight implants). Four patients were given palatal mucosal grafts to replace intraoral skin flaps around the dental implants (n=13), and the other seven had the skin flaps around the dental implants thinned (n=25) at the second stage of implantation of the osteointegrated teeth. All vascularised fibular osteoseptocutaneous flaps were successfully transferred, and all implants survived a mean (range) of 73 (33-113) months after occlusal functional loading. The mean (SD) marginal bone loss was 0.5 (0.3) mm on both mesial and distal sides in patients who had palatal mucosal grafts, but 1.8 (1.6) mm, and 1.7 (1.5) mm, respectively, on the mesial and distal sides in the patients who had had thinning of their skin flaps. This difference is significant (p=0.008) with less resorption of bone in the group who had palatal mucosal grafts. Palatal mucosa around the implants helps to reduce resorption of bone after functional loading of implants. Copyright © 2016 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  20. Gene expression profiles of cell adhesion molecules, matrix metalloproteinases and their tissue inhibitors in canine oral tumors.

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    Pisamai, Sirinun; Rungsipipat, Anudep; Kalpravidh, Chanin; Suriyaphol, Gunnaporn

    2017-08-01

    Perturbation of cell adhesion can be essential for tumor cell invasion and metastasis, but the current knowledge on the gene expression of molecules that mediate cell adhesion in canine oral tumors is limited. The present study aimed to investigate changes in the gene expression of cell adhesion molecules (E-cadherin or CDH1, syndecan 1 or SDC1, NECTIN2 and NECTIN4), matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs), in canine oral tumors, including benign tumors, oral melanoma (OM) and non-tonsillar oral squamous cell carcinoma (OSCC), by quantitative real-time reverse transcription PCR. When compared with the normal gingival controls, decreased CDH1, SDC1 and NECTIN4 expression levels were observed in OSCC and OM, reflecting a possible role as cell adhesion molecules and tumor suppressors in canine oral cancers in contrast to the upregulation of MMP2 expression. Downregulated MMP7 was specifically revealed in the OM group. In the late-stage OM, the positive correlation of MMP7 and CDH1 expression was noticed as well as that of SDC1 and NECTIN4. Enhanced TIMP1 expression was shown in all tumor groups with prominent expression in the benign tumors and the early-stage OM. MMP14 expression was notable in the early-stage OM. Higher MMP9 and TIMP1 expression was observed in the acanthomatous ameloblastoma. In conclusion, this study revealed that the altered expression of cell adhesion molecules, MMP7 and MMP2 was correlated with clinicopathologic features in canine oral cancers whereas TIMP1 and MMP14 expression was probably associated with early-stage tumors; therefore, these genes might serve as molecular markers for canine oral tumors. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. A retrospective investigation on canine papillomavirus 1 (CPV1 in oral oncogenesis reveals dogs are not a suitable animal model for high-risk HPV-induced oral cancer.

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    Ilaria Porcellato

    Full Text Available CPV1 (also called COPV is a papillomavirus responsible for oral papillomatosis in young dogs. The involvement of this viral type in oral oncogenesis has been hypothesized in oral squamous cell carcinomas (SCCs, but has never been investigated in other neoplastic and hyperplastic oral lesions of dogs. Aim of this study was to investigate the presence of CPV1 in different neoplastic and hyperplastic lesions in order to assess its role in canine oral oncogenesis; according to the results obtained, a second aim of the study was to define if the dog can be considered a valid animal model for oral high risk HPV-induced tumors. Eighty-eight formalin-fixed, paraffin-embedded (FFPE canine oral lesions including 78 oral tumors (papillomas, SCCs, melanomas, ameloblastomas, oral adenocarcinomas and 10 hyperplastic lesions (gingival hyperplasia were investigated with immunohistochemistry for the presence of papillomavirus L1 protein and with Real-Time PCR for CPV1 DNA. RT-PCR for RNA was performed on selected samples. All viral papillomas tested were positive for immunohistochemistry and Real-time PCR. In 3/33 (10% SCCs, viral DNA was demonstrated but no viral RNA could be found. No positivity was observed both with immunohistochemistry and Real-Time PCR in the other hyperplastic and neoplastic lesions of the oral cavity of dogs. Even though the finding of CPV1 DNA in few SCCs in face of a negative immunohistochemistry could support the hypothesis of an abortive infection in the development of these lesions, the absence of viral RNA points out that CPV1 more likely represents an innocent bystander in SCC oncogenesis. The study demonstrates a strong association between CPV1 and oral viral papillomas whereas viral contribution to the pathogenesis of other oral lesions seems unlikely. Moreover, it suggests that a canine model of CPV1 infection for HPV-induced oncogenesis could be inappropriate.

  2. A retrospective investigation on canine papillomavirus 1 (CPV1) in oral oncogenesis reveals dogs are not a suitable animal model for high-risk HPV-induced oral cancer.

    Science.gov (United States)

    Porcellato, Ilaria; Brachelente, Chiara; Guelfi, Gabriella; Reginato, Alice; Sforna, Monica; Bongiovanni, Laura; Mechelli, Luca

    2014-01-01

    CPV1 (also called COPV) is a papillomavirus responsible for oral papillomatosis in young dogs. The involvement of this viral type in oral oncogenesis has been hypothesized in oral squamous cell carcinomas (SCCs), but has never been investigated in other neoplastic and hyperplastic oral lesions of dogs. Aim of this study was to investigate the presence of CPV1 in different neoplastic and hyperplastic lesions in order to assess its role in canine oral oncogenesis; according to the results obtained, a second aim of the study was to define if the dog can be considered a valid animal model for oral high risk HPV-induced tumors. Eighty-eight formalin-fixed, paraffin-embedded (FFPE) canine oral lesions including 78 oral tumors (papillomas, SCCs, melanomas, ameloblastomas, oral adenocarcinomas) and 10 hyperplastic lesions (gingival hyperplasia) were investigated with immunohistochemistry for the presence of papillomavirus L1 protein and with Real-Time PCR for CPV1 DNA. RT-PCR for RNA was performed on selected samples. All viral papillomas tested were positive for immunohistochemistry and Real-time PCR. In 3/33 (10%) SCCs, viral DNA was demonstrated but no viral RNA could be found. No positivity was observed both with immunohistochemistry and Real-Time PCR in the other hyperplastic and neoplastic lesions of the oral cavity of dogs. Even though the finding of CPV1 DNA in few SCCs in face of a negative immunohistochemistry could support the hypothesis of an abortive infection in the development of these lesions, the absence of viral RNA points out that CPV1 more likely represents an innocent bystander in SCC oncogenesis. The study demonstrates a strong association between CPV1 and oral viral papillomas whereas viral contribution to the pathogenesis of other oral lesions seems unlikely. Moreover, it suggests that a canine model of CPV1 infection for HPV-induced oncogenesis could be inappropriate.

  3. Tooth apatite as a bone substitute: an experimental study and clinical applications

    International Nuclear Information System (INIS)

    Eun-Seok Kim; Pill-Hoon Choung

    1999-01-01

    The purpose of this study is to evaluate the usefulness of calcined teeth powder as biological apatite. The animal experiment was performed in 36 rabbits aging 6 weeks and weighing 1.6 kg. In experimental group, tooth apatite powder was implanted to 10 mm bony defects in diameter made on the cranial bone of the rabbits. As control groups, synthetic porous hydroxyapatite and resorbable type calcium carbonate were implanted to the defects of same size. Each group was sacrificed in 1, 2, 4, 6, 8, 12 weeks after the surgery. Specimens were prepared for decalcified samples and observed by a light microscope. And we also performed quantitative analysis of new bone formation through image analysis using computer. In clinical applications, we used tooth apatite alone or mixed with decalcified freeze-dried bone for reconstruction of bony defects in 15 patients undergone enucleation of cyst or ameloblastoma. The obtained results were as follows; 1) The powder of the calcined teeth was called as 'tooth apatite' and it seemed to have biocompatibility in rabbits and human. 2) In group of tooth apatite, after 4 weeks of operation, new bone directly bonded to the particles was observed. And in 12 weeks of it, new bone occupied most of the bony defects. In 6 weeks, resorption of the tooth apatite particles was observed. Thus the tooth apatite was regarded as one of resorbable apatite. 3) The group of tooth apatite showed new bone formation similar to the group of porous hydroxyapatite, but they were inferior to the group of resorbable calcium carbonate. 4) In clinical application, tooth apatite had biocompatibility and new bone formation was observed without any complication except for 1 case. So we think it is a useful bone substitute with osteoconductivity

  4. Epithelioid Multinodular Osteoblastoma of the Mandible: A Case Report and Review of Literature.

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    Kashikar, Shruti; Steinle, Michael; Reich, Renee; Freedman, Paul

    2016-06-01

    Epithelioid multinodular osteoblastoma is a rare variant of osteoblastoma characterized by numerous nodules of epithelioid osteoblasts surrounding bony trabeculae, as well as clusters of epithelioid osteoblasts without osteoid formation. It commonly occurs in the gnathic bones of the face and spine, and has a male predominance. To date, only 26 cases of epithelioid multinodular osteoblastoma have been reported and described in detail in the literature. Lucas et al. (Hum Pathol 25:117-134, 1994) described 43 cases of a variant of osteoblastoma that he termed epithelioid multifocal osteoblastoma. These both likely represent the same entity. Here, we report another case of this rare variant of osteoblastoma. An 18-year-old male patient presents with a periapical radiolucency in the region of vital tooth #30. The surgeon's differential diagnosis for this radiolucent lesion was ameloblastoma versus cyst. An incisional biopsy of the lesion revealed well-vascularized fibrous connective tissue containing a multinodular tumor composed of collections of epithelioid cells with osteoblastic differentiation surrounding zones of hyalinization and bony trabeculae. Multinucleated giant cells and rare typical mitotic figures were noted. Additionally present within the tumor were clusters of epithelioid osteoblasts without bony trabeculae. Residual immature viable bone trabeculae were noted surrounding the tumor. A diagnosis of epithelioid multinodular osteoblastoma was rendered. In this paper we present a rare case of epithelioid multinodular osteoblastoma of the mandible, provide a general review of the literature, and highlight the unique histological features that help differentiate this tumor from tumors classified as conventional osteoblastoma, aggressive osteoblastoma, pseudoanaplastic osteoblastoma and, most importantly, low-grade or osteoblastoma-like osteosarcoma.

  5. Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviors

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    Seyed Hossein Tabatabaei

    2016-11-01

    Full Text Available Background: Cystic lesions with odontogenic epithelial origin and similar clinicoradiographic appearance, show different clinical behaviors. Objective: To compare some factors related to cell proliferation and escape from apoptosis in epithelium covering two groups of odontogenic cystic lesions with different clinical behaviors. Methods: In this cross-sectional study 11 paraffin-embedded samples were selected of each lesions radicular cyst, dentigerous cyst, odontogenic keratocyst, and unicystic ameloblastoma. The sample underwent immunohistochemical staining for investigating the expression of ki-67 antigen and bcl-2 protein. Data analyzed with SPSS17 software and Kruskal–Wallis and chi-square statistical tests. Findings: Most of ki-67 positive cells were observed in parabasal layer of odontogenic keratocyst [35.50±26.29%; P=0.001]. The average of ki-67-LI was more in parabasal layer of aggressive group (26.80±37.79% compared to non-aggressive group (4.04±3.38%, was not being statistically significant. The highest average of bcl-2-LI was 95±6.70% in basal layer of odontogenic keratocyst (P=0.001. In all layers, the average of bcl-2-LI was more in aggressive lesions compared to non-aggressive ones and the highest amount was found in basal layer (72.45±3.94×10% which was statistically significant (P=0.001. Conclusion: According to the results of this study, more expression of the markers related to escape from apoptosis in aggressive lesions group compared to non-aggressive group, suggests that escape from apoptosis had a more critical role in aggressive behavior of odontogenic cystic lesions.

  6. Prevention of collapse of the contralateral half of the mandible after hemimandibulectomy: Our experience in a low-resource center

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    Charles E Anyanechi

    2017-01-01

    Full Text Available Background: The management of pathologic lesions of the mandible includes plans for the reconstruction of the resultant defect to give the patients optimal surgical reconstructive and prosthetic results. Objective: To evaluate the degree of deviation of the contralateral half of the mandible toward the surgical defect after hemimandibulectomy and intermaxillary fixation (IMF. Patients and Methods: This is a 9-year prospective single-blinded clinical study conducted at the Dental and Maxillofacial Surgery Clinic of our institution. Information obtained from the patients included age, gender, type of mandibular lesion, method of wound closure, duration of IMF, temporo-mandibular joint (TMJ symptom(s, and the deviation toward the surgical defect of the remnant contralateral half of the mandible, measured in centimeter at maximum mouth opening. Results: Ninety-six patients, unevenly distributed according to their duration of tolerance of IMF, were studied. The age of the patients ranged from 29 to 57 years with an overall mean age of 42.6 ± 5.1 years. There were 72 males and 24 females with a male-to-female ratio of 3:1 (P = 0.001. The lesions that were extirpated were all benign, and ameloblastoma was the most common tumor (P = 0.001. The shorter the duration of IMF, the greater the deviation of the mandibular mid-line toward the surgical defect (P = 0.001. Conclusion: This study shows that there is a deviation of mid-line of the residual mandible toward the surgical defect after hemimandibulectomy, even after its immobilization with IMF for 4–12 weeks. IMF is still useful in the prevention of mandibular collapse after hemimandibulectomy.

  7. OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions

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    Abhishek Banerjee

    2016-01-01

    Full Text Available Context (Background: Stem cells are a unique subpopulation of cells in the human body with a capacity to initiate differentiation into various cell lines. Tumor stem cells (TSCs are a unique subpopulation of cells that possess the ability to initiate a neoplasm and sustain self-renewal. Epithelial stem cell (ESC markers such as octamer-binding transcription factor 4 (OCT4 and sex-determining region Y (SRY-box 2 (SOX2 are capable of identifying these stem cells expressed during the early stages of tooth development. Aims: To detect the expression of the stem cell markers OCT4 and SOX2 in the normal odontogenic tissues and the odontogenic cysts and tumors. Materials and Methods: Paraffin sections of follicular tissue, radicular cyst, dentigerous cyst, odontogenic keratocyst, ameloblastoma, adenomatoid odontogenic tumor, and ameloblastic carcinoma were obtained from the archives. The sections were subjected to immunohistochemical assay by the use of mouse monoclonal antibodies to OCT4 and SOX2. Statistical Analysis: The results were evaluated by descriptive analysis. Results: The results show the presence of stem cells in the normal and lesional tissues with these stem cell identifying markers. SOX2 was found to be more consistent and reliable in the detection of stem cells. Conclusion: The stem cell expressions are maintained in the tumor transformation of tissue and probably suggest that there is no phenotypic change of stem cells in progression from normal embryonic state to its tumor component. The quantification and localization reveals interesting trends that indicate the probable role of the cells in the pathogenesis of the lesions.

  8. [Study on accuracy of virtual surgical planning in free fibula mandibular reconstruction by using SurgiCase software].

    Science.gov (United States)

    Yan, Guangqi; Wang, Xue; Tan, Xuexin; Wang, Xukai; Yang, Mingliang; Lu, Li

    2013-08-01

    To evaluate the directional significance of SurgiCase software in free fibula mandibular reconstruction. Between September 2010 and March 2012, 10 patients with mandibular defect underwent free fibula mandibular reconstruction. There were 7 males and 3 females, with an age range of 19-43 years (mean, 27 years). The extent of lesions was 7 cm x 5 cm to 16 cm x 8 cm. In each case, three-dimensional spiral CT scan of the maxilla, mandible, and fibula was obtained before surgery. The CT data were imported into the SurgiCase software and the virtual surgery planning was performed. After that, the mandibular rapid prototyping was made according to customized design. The reconstruction surgery was then carried out using these preoperative data. During actual surgery, the extent of mandibular defect was from 6 cm x 3 cm to 16 cm x 5 cm; the length of fibula which was used to reconstruct mandible was 6-17 cm; and the area of flap was from 6 cm x 5 cm to 16 cm x 6 cm. Preoperative data could not be applied because the intraoperative size of tumor was larger than preoperative design in 1 case of mandibular ameloblastoma, and the fibula was shaped according to the actual osteotomy location; operations were performed successfully according to preoperative design in the other 9 patients. The operation time was 5-7 hours (mean, 6 hours). Primary healing of incision was obtained, without early complications. Ten patients were followed up 1 year. At last follow-up, 8 patients were satisfactory with the appearance and 2 patients complained with unsatisfied wide facial pattern. The panoramic radiograghs showed good bone healing. The range of mouth opening was 2.5-3.5 cm. SurgiCase software can provide precise data for free fibula mandibular reconstruction during surgery. It can be applied widely in clinic.

  9. Head and neck reconstruction using infrahyoid myocutaneous flaps

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    Alfio José Tincani

    Full Text Available CONTEXT AND OBJECTIVE: The use of pedicled myocutaneous flaps in head and neck reconstruction is widely accepted. Here we describe our experience with infrahyoid flaps (IHFs employed to cover surgical defects in the oral cavity and oropharynx in patients with benign and malignant tumors. The aim was to evaluate the success rate for infrahyoid myocutaneous flap procedures performed at a single institution. DESIGN AND SETTING: Retrospective study, at the Head and Neck Surgery Service, Unicamp. METHODS: Fourteen IHFs were used to reconstruct surgical defects in eleven men (78.5% and three women (21.5% with a mean age of 66.4 years. The anterior floor of the mouth was reconstructed in nine patients (64.2%, the base of tongue in three (21.4%, the lateral floor in one (7.1%, and the retromolar area (7.1% in one. Thirteen patients (92.8% had squamous cell carcinoma (SCC and one (7.2% ameloblastoma. The disease stage was T3 in eight (61.5% of the SCC cases and T4 in five (38.5%. RESULTS: No patient presented total flap loss or fistula. The most common complication was epidermolysis, which delayed the beginning of oral ingestion. The patients with SCC received postoperative radiotherapy without major consequences to the flap. CONCLUSION: IHF is a safe and reliable procedure for reconstructing head and neck surgical defects. Due to its thinness and malleability, its use for oral cavity and oropharynx defects provides favorable cosmetic and functional outcomes. Complications, when present, are easy to manage.

  10. An audit of oral diseases at a Nairobi centre, 2000-2004.

    Science.gov (United States)

    Dimba, E A O; Gichana, J; Limo, A K; Wakoli, K A; Chindia, M L; Awange, D O

    2007-12-01

    To describe oral diseases diagnosed in an urban referral centre in Kenya in terms of age, gender and anatomical distribution and to compare this with reports in the literature. A retrospective histopathological audit. Oral Pathology Laboratory at the University of Nairobi Dental Hospital, a tertiary referral centre in Kenya. 548 (53.83%) patients were diagnosed with oral and maxillofacial tumours. Benign tumours (mean age +/- SD = 29.93 +/- 18.27 years) peaked in the third decade and tended to affect men at a younger age (p = 0.001). The most common benign tumour was the ameloblastoma (50.23%: n = 109), which predominantly affected the mandible, and also occurred in male patients at a younger age (p = 0.023). Peak incidences for malignant disease were observed in the sixth decade (mean age +/- SD = 46.94 +/- 18.99 years). Oral squamous cell carcinoma (OSCC) was the most common malignant tumour (59.55%; n = 187); occurring in the tongue, floor of the mouth, buccal mucosa and palate. 10.30% of OSCC occurred in patients under 40 years of age. 147 patients presented with cysts, which were mainly (68.10%) of odontogenic origin. Reactive lesions, infections, salivary gland diseases and autoimmune conditions constituted 26.60% of the case load. The tendency of oral squamous cell carcinoma to occur in younger age groups may be an indication of carcinogenic factors that could be peculiar to this population. There is an urgent need for the expansion of reporting systems for oral diseases as an integral part of development of appropriate strategies in the improvement of general health in Kenya.

  11. Avaliação histopatológica e histoquímica das células claras em lesões odontogênicas císticas

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    Cláudia Roberta Leite Vieira de Figueiredo

    Full Text Available Alterações degenerativas e metaplásicas são usualmente observadas no revestimento epitelial dos cistos odontogênicos. Nos tumores odontogênicos estes processos são considerados mais raros. Objetivo: demonstrar a presença de células claras no revestimento epitelial de uma série de lesões odontogênicas císticas. Métodos: realizou-se um estudo descritivo, em uma amostra de 22 lâminas histológicas, do total de lesões odontogênicas císticas procedentes do Laboratório de Patologia Bucal da Universidade Federal da Paraíba. As lâminas foram examinadas por dois avaliadores previamente calibrados e os blocos parafinados correspondentes às lesões em que se observaram células claras, foram novamente processados, e as lâminas foram coradas pela técnica do Ácido Periódico de Schiff após digestão pela diástase e avaliadas quanto à presença de células mucosas. Resultados: células contendo mucina estavam presentes em 12 (54,5 % das lesões coradas pelo Ácido Periódico de Schiff com diástase. Células mucosas foram observadas em 5 (41,66 % dos 12 casos de cistos radiculares, 1 (50 % dos 2 casos de tumores odontogênicos ceratocísticos, nos 4 (100 % casos de cistos dentígeros e em 2 (100 % casos de ameloblastomas unicísticos. Células claras foram muitas vezes observadas em áreas de inflamação. Conclusão: na amostra estudada, células mucosas puderam ser evidenciadas ocasionalmente no epitélio de lesões odontogênicas císticas.

  12. Does cytokine profiling of aspirate from jaw cysts and tumors have a role in diagnosis?

    Science.gov (United States)

    Kolokythas, Antonia; Karas, Maria; Sarna, Thomas; Flick, William; Miloro, Michael

    2012-05-01

    The objective of the present study was twofold: first, to assess aspirates for use in cytokine profiling and second, to initiate pilot analyses to determine whether the cytokine profiling can serve as an aid in the diagnosis of jaw lesions. The aspirates from 12 benign odontogenic cysts and tumors of the jaw were collected and randomized, and a formal incisional biopsy was performed to establish the tissue diagnosis. The biopsies revealed keratocystic odontogenic tumor, ameloblastoma, and dentigerous cyst. The cystic aspirate was analyzed using the Q-Plex Human Cytokine Screen to detect cytokine expression and determine the level of expression for each pathologic entity. An array of 16 cytokines was investigated, including interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-15, IL-17, IL-23, interferon-γ, tumor necrosis factor (TNF)-α, and TNF-β. Tables were developed to determine the ratio of expression for the candidate cytokine pairs that were differentially expressed among the 3 pathologic entities encountered. One-way analysis of variance was used to search for significant differences in the ratio of expression of the candidate pairs among the 3 entities. Cytokines expressed by the 3 distinct jaw lesions were detected in the aspirate without the need for tissue biopsy. Cytokine profiling of these entities is possible owing to differential expression of the various cytokines studied. The ratio of expression was significant (P lesion type for the 15 cytokine pairs. Cytokines, expressed by the 3 groups of jaw lesions, can be detected in the cystic aspirate, and a comparison of the ratio of the expression of the aspirates demonstrated a differential expression pattern of cytokines among the 3 groups. These ratios could assist in establishing a prompt and accurate diagnosis of lesions that might be difficult to discern clinically and radiographically. The use of a simple, minimally invasive aspiration procedure can help to

  13. Concepto actual, diagnóstico y tratamiento del tumor odontogénico adenomatoide. Reporte de un caso

    Directory of Open Access Journals (Sweden)

    Manuel Escalante Fontalvo

    2012-01-01

    Full Text Available El tumor odontogénico adenomatoide (TOA es una lesión clasificada por la Organización Mundial de la Salud (OMS dentro de los tumores odontogénicos con participación del ectomesénquima, ya que puede contener, además del epitelio, tejido calcificado en su interior, que muestra una morfología histológica muy peculiar. Es un tumor benigno de baja prevalencia que compromete solo el 0,1% de los tumores y quistes de los maxilares, con una muy baja tendencia a la recidiva; es común en pacientes jóvenes, generalmente mujeres, de mayor presentación en el maxilar superior, asintomático, de crecimiento lento y poco invasivo; el cual se puede semejarse a otras lesiones odontogénicas de mayor agresividad como el quiste dentígero y el ameloblastoma, entre otros benignos como el odontoma complejo y adenomas pleomórficos. A menudo se observa como una lesión radiolúcida de aspecto quístico unilocular, asociada a órganos dentales incluidos, usualmente dientes caninos. Su localización clásica nos orienta al diagnóstico y su patrón histológico ductiforme (apariencia basaloide con estructuras glanduliformes, calcificaciones esferulares y presencia de abundante material amiloide que facilitan su reconocimiento microscópico es muy propio de esta lesión tumoral. Se presenta un caso de un paciente de 12 años de edad, asintomática y asimétrica facial, de sexo femenino, con un diagnóstico definitivo por biopsia de TOA en la región anterior del maxilar superior asociado a un órgano dental 23 incluido; intervenido quirúrgicamente, colocando material de injerto como sustituto óseo, teniendo en cuenta estado general del paciente, diagnósticos diferenciales, características radiográficas, tomográficas y clínicas.

  14. A statistical study of the maxillofacial diseases by radiograms

    International Nuclear Information System (INIS)

    Chung, Yoo Tai; Lee, Sang Chull

    1974-01-01

    This report based on 300 cases of serious diseases in maxillofacial region by radiograms seen at the department of dental radiodontics, infirmary school of dentistry, Kyung Hee University from October 1971 to August 1974. The maxillofacial diseases were analysed upon the following items, such as 1) the frequency of dominant diseases, 2) sex-ratio of male to female, 3) predominant region of diseases, 4) comparison with the age, 5) the incidence of diseases in relative to the individual teeth. The results were obtained as follows. 1) Among the total of 300 cases of the patients, the frequency of dominant diseases of patients were fractures of facial bone (44.3 ± 2.87%), inflammatory diseases (22.7 ± 2.39%), cysts (11.1 ± 1.62%), tumors(10.7 ± 1.77%), maxillary sinusitis (7.9 ± 1.56%), temporomandibular joint disorders(3.3 ± 1.05%) in the order. 2) The sex ratio of male to female in occurrence of jaw fractures were 7.3 : 1, temporomandibular joint disorders were 2.1 : 1, inflammatory diseases were 1.8:1, maxillary sinusitis were 1.7 : 1, but tumors were 1: 1, while cysts were 1:1 .2 in sex difference.3) The predominant region of mandibular fractures were symphysis (17.3 ± 3.27%), canine region (15.0 ± 3.09%), and angle region(14.3 ± 3.04%) in the order. Inflammatory diseases were occurred frequently in mandible and it's left side were a little dominant. Odontogenic cysts were observed frequently in maxilla, but regardless of right and left. Carcinomas were involved most frequently in maxilla, while sarcomas and ameloblastomas in mandible. Frequency of the maxillary sinusitis w ere dominant in right side and molar area, also temporomandibular joint disorders were dominant in right side. 4) To study comparing with the age, jaw fractures showed the highest ratio at the 2nd decade (32.3 ± 4.06%), and 3rd decade (27.8 ± 3.89%), 4th decade (19.6 ± 3.44%), 6th decade (9.0 ± 2.47%), 5th decade(6.0 ± 2.06%), lst decade(5.3 ± 1.95%) in the order. But 7th decade

  15. Obturator Definitive Mandibula Post Hemimandibulectomy Sinistra

    Directory of Open Access Journals (Sweden)

    Paul Sugiyo

    2012-12-01

    Full Text Available Latar Belakang. Operasi bedah pemotongan mandibula pada kasus tumor jinak maupun tumor ganas dapat menyebabkan deviasi mandibula. Tindakan perawatan bedah tergantung pada lokasi dan perluasan tumor mandibula, tindakan perawatan bedah tersebut meliputi bedah marginal, segmental, hemimandibulectomy, dan total mandibulectomy. Tujuan. Makalah ini menjelaskan tentang perawatan rehabilitasi dengan obturator definitive mandibula. Para klinisi harus menunggu masa penyembuhan yang sempurna sebelum disarankan untuk dibuatkan onturator definitive mandibula. Sejak awal penyembuhan diperlukan intervensi prostodontis untuk mencegah deviasi mandibula. Protesa ini membantu pergerakan mandibula secara normal tanpa terjadi penyimpangan pada fungsi bicara dan pengunyahan. Laporan Kasus. Pada bulan Maret 2012, pasien laki-laki, berumur 46 tahun datang ke RSGM Bagian Prostodonsia atas rujukan dari RS. Dr. Sardjito Bagian Bedah Mulut setelah dilakukan operasi tumor ameloblastoma mandibula sinistra (post hemimandibulectomy mandibula sinistra dengan pemasangan plat rekonstruksi tiga bulan sebelumnya untuk dibuatkan obturator definitive mandibula. Pasien mengeluh bibir bawah sebelah kiri sering tergigit, fungsi bicara, fungsi pengunyahan, dan penampilannya terganggu. Hasil. Setelah dilakukan perawatan dengan memakai obturator definitive mandibula dalam kurun waktu 8 bulan, hingga saat ini hasil perawatan ini dapat mengembalikan fungsi bicara, fungsi pengunyahan, dan fungsi estetik sehingga pasien merasa lebih nyaman dan percaya diri. Kesimpulan. Deviasi mandibula setelah operasi hemimandibulectomy diatasi dengan bedah rekonstruksi menggunakan plat rekonstruksi, kemudian segera setelah penyembuhan perlu melibatkan prostodontis untuk pemasangan obturator definitive mandibula.    Background. Surgical restion of the mandible due to presence of benign or malignant tumor is the common cause of the mandibular deviation. Depending upon the location and extent of the tumor in