WorldWideScience

Sample records for alep diva eensoo

  1. The Automated Logistics Element Planning System (ALEPS)

    Science.gov (United States)

    Schwaab, Douglas G.

    1992-01-01

    ALEPS, which is being developed to provide the SSF program with a computer system to automate logistics resupply/return cargo load planning and verification, is presented. ALEPS will make it possible to simultaneously optimize both the resupply flight load plan and the return flight reload plan for any of the logistics carriers. In the verification mode ALEPS will support the carrier's flight readiness reviews and control proper execution of the approved plans. It will also support the SSF inventory management system by providing electronic block updates to the inventory database on the cargo arriving at or departing the station aboard a logistics carrier. A prototype drawer packing algorithm is described which is capable of generating solutions for 3D packing of cargo items into a logistics carrier storage accommodation. It is concluded that ALEPS will provide the capability to generate and modify optimized loading plans for the logistics elements fleet.

  2. The Automated Logistics Element Planning System (ALEPS)

    Science.gov (United States)

    Schwaab, Douglas G.

    1992-01-01

    ALEPS, which is being developed to provide the SSF program with a computer system to automate logistics resupply/return cargo load planning and verification, is presented. ALEPS will make it possible to simultaneously optimize both the resupply flight load plan and the return flight reload plan for any of the logistics carriers. In the verification mode ALEPS will support the carrier's flight readiness reviews and control proper execution of the approved plans. It will also support the SSF inventory management system by providing electronic block updates to the inventory database on the cargo arriving at or departing the station aboard a logistics carrier. A prototype drawer packing algorithm is described which is capable of generating solutions for 3D packing of cargo items into a logistics carrier storage accommodation. It is concluded that ALEPS will provide the capability to generate and modify optimized loading plans for the logistics elements fleet.

  3. Le pin d'Alep et l'incendie - Sylviculture préventive

    OpenAIRE

    2013-01-01

    La sylviculture préventive consiste à compléter les interventions sylvicoles traditionnelles par des mesures propres à rendre les peuplements moins sensibles au feu. L'objet de cette fiche est de définir les objectifs de la sylviculture préventive et d'en décliner les caractéristiques et les différentes modalités dans les peuplements de pin d'Alep. Il s'agit aussi de déterminer les situations où les objectifs de la sylviculture préventive ne sauraient être atteints efficacement.

  4. Anforderungen an die Detektoren von DIVA.

    Science.gov (United States)

    Wagner, S. J.

    Neben dem optischen System sind die Detektoren in der Fokalebene die wichtigste Komponente des Instruments eines astrometrischen Minisatelliten. Einer möglichst optimalen Auswahl des Detektorsystems und seiner Operationsmodi kommt daher eine entscheidende Bedeutung zu. In diesem Beitrag werden die Anforderungen vorgestellt, die sich zum gegenwärtigen Stand der Planung eines Interferometrie-Minisatelliten für astrometrische und photometrische Untersuchungen ergeben. Sie basieren auf dem grundlegenden Missionskonzept für die DIVA Mission (Bastian et al., 1996) und dem an der Landessternwarte Heidelberg zusammen mit dem ARI Heidelberg und DSS Ottobrunn erarbeiteten optischen Konzept des Instruments.

  5. A dádiva organizacional: Dádiva à distância e circuitos de troca

    Directory of Open Access Journals (Sweden)

    Philippe Steiner

    Full Text Available Resumo Este artigo apresenta as características da dádiva organizacional: uma forma particular de doação na qual uma ou mais organizações colocam-se entre o doador e o donatário. Após uma caracterização dessa doação em comparação com a dádiva maussiana, o artigo examina três características da dádiva organizacional: o papel desempenhado pela organização que separa ao mesmo tempo que conecta; os “circuitos de comércio” pelos quais passam as dádivas organizacionais e, por fim, a questão levantada pela dificuldade de reciprocar a dádiva na ausência de um contato direto entre doador e donatário. Por fim, o artigo sublinha a importância dessa dádiva para compreender a formação de uma solidariedade à distância entre os seres humanos.

  6. Divas en la brega: apuntes para el estudio de Las divas de mi barrio de Carmen Montañez

    Directory of Open Access Journals (Sweden)

    Violeta Lorenzo-Feliciano

    2016-01-01

    Full Text Available En este trabajo analizaré la manera en que Montañez redefine el concepto de diva en su colección de cuentos Las divas de mi barrio. Arguyo que su redefinición valoriza la función de personajes femeninos que no sólo difieren físicamente de las ideas de belleza que se asocian con una diva sino que pertenecen a la clase trabajadora del país donde se desarrolla la acción. Por lo tanto, en estos cuentos se rinde tributo a otras formas de femineidad y de conocimientos que tienden a quedar excluidos por no ir a tono con las conductas y estéticas que afianzan el machismo.

  7. Fisheries studies and stock evaluation of shrimp scad, Alepes djedaba (Teleostei:Carangidae) caught from Arabian Gulf

    Institute of Scientific and Technical Information of China (English)

    Medhat Abdel Barr; Amany Mohammed Osman; Hayam Abdulla Al Abdulhadi

    2014-01-01

    Objective: To evaluate the stock of Alepes djedaba (A. djedaba) by describing the length composition, growth parameters, mortality rates of A. djedaba captured in Arabian Gulf off Saudi Arabia and adopting yield per recruit and biomass per recruit models. Methods:A random sample of 490 fish representing a moderate range of total lengths (16.5-32.4cm) and weights (60-410 g) were sampled in Arabian Gulf off Dammam, Saudi Arabia during the period from August 2008 to July 2009. LFD5 software was used for estimation of growth parameters. Total mortality was calculated using the length converted catch curve. Natural mortality was estimated using Pauly and David's formula. Fishing mortality was computed by subtracting natural mortality from total mortality. Per recruit analysis was made using Beverton and Holt model. Results:Length-frequency analysis revealed four peaks and the length range from 22 cm to 27 cm dominated the catch, constituting about 71% of the catch. Values of the von Bertalanffy growth parameters were computed using LFD5 software as follows: the asymptotic length (Lg)=41.71 cm, curvature parameter (K)= 0.36 year-1, and hypothetic age at zero length (t0) = -0.76 year. The total mortality (Z) was estimated as 2.07 year-1, and natural mortality was 0.8 year-1. Fishing mortalityF=1.27 year-1 , which was higher than F0.1 (0.3 year-1), FSB(50) (0.59 year-1) and FSB(40) (0.86 year-1). Atthe current levels of fishing and natural mortality, the biomass per recruit is 34% of the virgin biomass. Conclusions:These may indicate an overexploitation state of the fisheries of A. djedaba in Arabian Gulf .

  8. The Deep Interferometric VSOP--Arecibo Survey (DIVAS)

    Science.gov (United States)

    Edwards, P. G.; Hirabayashi, H.; Ulvestad, J. S.; Salter, C.; Ghosh, T.; Gurvits, L. I.; Fomalont, E. B.

    2009-08-01

    The Deep Interferometric VSOP-Arecibo Survey (DIVAS) program used space VLBI observations of a faint sample of flat-spectrum sources made with the HALCA satellite and the Arecibo telescope to provide the highest possible space VLBI sensitivity at 5 GHz. The main aim of the survey is to compare statistical results on the source structures (e.g., brightness temperatures, sizes, visibilities) with results from the VLBI Space Observatory Programme (VSOP) Survey, to determine whether there are systematic differences in the compact structures of sources selected from samples with limiting flux densities differing by over an order of magnitude.

  9. O Linux e a perspectiva da dádiva

    Directory of Open Access Journals (Sweden)

    Renata Apgaua

    2004-06-01

    Full Text Available O objetivo deste trabalho é analisar o surgimento e consolidação do sistema operacional Linux em um contexto marcado pela hegemonia de sistemas operacionais comerciais, sendo o Windows/Microsoft o exemplo paradigmático. O idealizador do Linux optou por abrir o seu código-fonte e oferecê-lo, gratuitamente, na Internet. Desde então, pessoas de diversas partes do mundo têm participado do seu desenvolvimento. Busca-se, assim, através deste estudo, analisar as características desse espaço de sociabilidade, onde as trocas apontam para outra lógica que não a do mercado. A proposta de compreender os laços sociais no universo Linux, a partir da perspectiva da dádiva, acaba remetendo a outra discussão, que também merecerá atenção nesse estudo, qual seja: a atualidade da dádiva. Releituras de Mauss, feitas por Godbout e Caillé, indicam que a dádiva, em seu "sistema de transformações", encontra-se presente nas sociedades contemporâneas, mas não apenas nos interstícios sociais, conforme afirmava o próprio Mauss.This work's goal is to analyze the appearance and consolidation of the Linux operational system in a context marked by the hegemony of commercial operational systems, taking the Windows/Microsoft as the paradigmatic example. The creator of Linux chose to make it open-source and offer it free of charge, in the Internet. Since then, people from the various parts of the world have participated in its development. This study, therefore, seeks to analyse the features of this space of sociability, where the exchange points to another logic different of that one adopted by the market. The proposal of comprehending the social ties of the Linux universe through the perspective of gift ends up sending us into another discussion, which will also deserve attention in this study, that would be: the recentness of gift. Re-interpretations of Mauss, made by Godbout and Caillé, indicate that gif, in its "changing system", is present in

  10. An E2-Substituted Chimeric Pestivirus With DIVA Vaccine Properties

    DEFF Research Database (Denmark)

    Rasmussen, Thomas Bruun; Uttenthal, Åse; Nielsen, Jens

    An advantage of the use of chimeric pestiviruses as modified live vaccines against classical swine fever (CSF) resides in their capacity to be manipulated to achieve the characteristics desired for safe and efficacious DIVA vaccines. We have recently generated a new chimeric virus, Riems26_E2gif...... engineered specifically for this purpose. The E2-substituted Riems26_E2gif was derived by homologues recombination of the complete E2 protein encoding genome region from Border disease strain Gifhorn into a bacterial artificial chromosome (BAC) harbouring the genome of the CSFV vaccine strain C......-Riems. The virulence, immunogenicity and vaccine properties of Riems26_E2gif were tested in a vaccine-challenge experiment in pigs. Riems26_E2gif vaccinated pigs could be differentiated from infected pigs using a CSFV-E2 specific ELISA. Following challenge infection with highly virulent CSFV strain Koslov, all...

  11. Uporaba metode električne upornosti tal na primeru Divaške jame = Application of electrical resistivity imaging on Divaška jama cave

    Directory of Open Access Journals (Sweden)

    Andrej Mihevc

    2011-01-01

    Full Text Available Electrical resistivity imaging (ERI is a widely used tool in geophysical survey ofvarious subsurface structures. To establish its applicability for karst subsurface, ERIwas conducted in Divača karst above caves of Trhlovca and Divaška jama and in itscontinuation as denuded cave on slopes of Radvanj collapse doline. Empty cave passageswere not detected with ERI as electrical resistivity difference between voids and highlyresistive carbonate bedrock is too small. On the other hand, denuded caves and cavesections, filled with loamy material, can be clearly distinguished.

  12. Characterization of a novel interaction between Bcl-2 members Diva and Harakiri.

    Directory of Open Access Journals (Sweden)

    Lorenzo Sborgi

    Full Text Available Interactions within proteins of the Bcl-2 family are key in the regulation of apoptosis. The death-inducing members control apoptotic mechanisms partly by antagonizing the prosurvival proteins through heterodimer formation. Structural and biophysical studies on these complexes are providing important clues to understand their function. To help improve our knowledge on protein-protein interactions within the Bcl-2 family we have studied the binding between two of its members: mouse Diva and human Harakiri. Diva has been shown to perform both prosurvival and killing activity. In contrast, Harakiri induces cell death by interacting with antiapoptotic Bcl-2 members. Here we show using ELISA and NMR that Diva and Harakiri can interact in vitro. Combining the NMR data with the previously reported three-dimensional structure of Diva we find that Harakiri binds to a specific region in Diva. This interacting surface is equivalent to the known binding area of prosurvival Bcl-2 members from the reported structures of the complexes, suggesting that Diva could function at the structural level similarly to the antiapoptotic proteins of the Bcl-2 family. We illustrate this result by building a structural model of the heterodimer using molecular docking and the NMR data as restraints. Moreover, combining circular dichroism and NMR we also show that Harakiri is largely unstructured with residual (13% α-helical conformation. This result agrees with intrinsic disorder previously observed in other Bcl-2 members. In addition, Harakiri constructs of different length were studied to identify the region critical for the interaction. Differential affinity for Diva of these constructs suggests that the amino acid sequence flanking the interacting region could play an important role in binding.

  13. Nota sobre Marcel Mauss e o ensaio sobre a dádiva

    Directory of Open Access Journals (Sweden)

    Lanna Marcos

    2000-01-01

    Full Text Available Este artigo analisa a obra clássica de M. Mauss, Ensaio sobre a dádiva, à luz de desenvolvimentos recentes da Antropologia. Salienta como contribuição de Mauss o entendimento da dimensão política da troca de dádivas, assim como a sugestão de sua universalidade, posteriormente demonstrada por Lévi-Strauss, constituir-se em princípio formal-abstrato, e não num fato empírico-concreto. A partir desse princípio, avalia a tese segundo a qual a dádiva é fundamento de toda sociabilidade e comunicação humanas, assim como sua presença e sua diferente institucionalização em várias sociedades analisadas por Mauss, capitalistas e não-capitalistas.

  14. Application of electrical resistivity imaging on Divaška jama cave

    Directory of Open Access Journals (Sweden)

    Andrej Mihevc

    2011-12-01

    Full Text Available Electrical resistivity imaging (ERI is a widely used tool in geophysical survey of various subsurface structures. To establish its applicability for karst subsurface, ERI was conducted in Divača karst above caves of Trhlovca and Divaška jama and in its continuation as denuded cave on slopes of Radvanj collapse doline. Empty cave passages were not detected with ERI as electrical resistivity difference between voids and highly resistive carbonate bedrock is too small. On the other hand, denuded caves and cave sections, filled with loamy material, can be clearly distinguished.

  15. Diva software, a tool for European regional seas and Ocean climatologies production

    Science.gov (United States)

    Ouberdous, M.; Troupin, C.; Barth, A.; Alvera-Azcàrate, A.; Beckers, J.-M.

    2012-04-01

    Diva (Data-Interpolating Variational Analysis) is a software based on a method designed to perform data-gridding (or analysis) tasks, with the assets of taking into account the intrinsic nature of oceanographic data, i.e., the uncertainty on the in situ measurements and the anisotropy due to advection and irregular coastlines and topography. The Variational Inverse Method (VIM, Brasseur et al., 1996) implemented in Diva consists in minimizing a variational principle which accounts for the differences between the observations and the reconstructed field, the influence of the gradients and variability of the reconstructed field. The resolution of the numerical problem is based on finite-element method, which allows a great numerical efficiency and the consideration of complicated contours. Along with the analysis, Diva provides also error fields (Brankart and Brasseur, 1998; Rixen et al., 2000) based on the data coverage and noise. Diva is used for the production of climatologies in the pan-European network SeaDataNet. SeaDataNet is connecting the existing marine data centres of more than 30 countries and set up a data management infrastructure consisting of a standardized distributed system. The consortium has elaborated integrated products, using common procedures and methods. Among these, it uses the Diva software as reference tool for climatologies computation for various European regional seas, the Atlantic and the global ocean. During the first phase of the SeaDataNet project, a number of additional tools were developed to make easier the climatologies production for the users. Among these tools: the advection constraint during the field reconstruction through the specification of a velocity field on a regular grid, forcing the analysis to align with the velocity vectors; the Generalized Cross Validation for the determination of analysis parameters (signal-to-noise ratio); the creation of contours at selected depths; the detection of possible outliers; the

  16. Data-Interpolating Variational Analysis (DIVA) software : recent development and application

    Science.gov (United States)

    Watelet, Sylvain; Beckers, Jean-Marie; Barth, Alexander; Back, Örjan

    2016-04-01

    The Data-Interpolating Variational Analysis (DIVA) software is a tool designed to reconstruct a continuous field from discrete measurements. This method is based on the numerical implementation of the Variational Inverse Model (VIM), which consists of a minimization of a cost function, allowing the choice of the analysed field fitting at best the data sets. The problem is solved efficiently using a finite-element method. This statistical method is particularly suited to deal with irregularly-spaced observations, producing outputs on a regular grid. Initially created to work in a two-dimensional way, the software is now able to handle 3D or even 4D analysis, in order to easily produce ocean climatologies. These analyses can easily be improved by taking advantage of the DIVA's ability to take topographic and dynamic constraints into account (coastal relief, prevailing wind impacting the advection,...). DIVA is an open-source software which is continuously upgraded and distributed for free through frequent version releases. The development is funded by the EMODnet and SeaDataNet projects and include many discussions and feedback from the users community. Here, we present two recent major upgrades : the data weighting option and the bottom-based analyses. Since DIVA works with a diagonal observation error covariance matrix, it is assumed that the observation errors are uncorrelated in space and time. In practice, this assumption is not always valid especially when dealing e.g. with cruise measurements (same instrument) or with time series at a fixed geographic point (representativity error). The data weighting option proposes to decrease the weights in the analysis of such observations. Theses weights are based on an exponential function using a 3D (x,y,t) distance between several observations. A comparison between not-weighted and weighted analyses will be shown. It has been a recurrent request from the DIVA users to improve the way the analyses near the ocean bottom

  17. DIVA vaccine properties of the live chimeric pestivirus strain CP7_E2gif

    DEFF Research Database (Denmark)

    von Rosen, Tanya; Rangelova, Desislava Yordanova; Nielsen, Jens

    2014-01-01

    Live modified vaccines to protect against classical swine fever virus (CSFV), based on chimeric pestiviruses, have been developed to enable serological Differentiation of Infected from Vaccinated Animals (DIVA). In this context, the chimeric virus CP7_E2gif vaccine candidate is unique as it does...... not include any CSFV components. In the present study, the DIVA vaccine properties of CP7_E2gif were evaluated in comparison to the conventional live attenuated Riemser C-strain vaccine. Sera and tonsil samples obtained from pigs immunised with these two vaccines were analysed. No viral RNA was found in serum...... after vaccination with CP7_E2gif, whereas some serum samples from C-strain vaccinated animals were positive. In both vaccinated groups, individual viral RNA-positive tonsil samples were detected in animals euthanised between 7 and 21 days post vaccination. Furthermore, serum samples from these animals...

  18. Data-Interpolating Variational Analysis (DIVA) software : recent development and application

    Science.gov (United States)

    Watelet, Sylvain; Barth, Alexander; Troupin, Charles; Ouberdous, Mohamed; Beckers, Jean-Marie

    2014-05-01

    The Data-Interpolating Variational Analysis (DIVA) software is a tool designed to reconstruct a continuous field from discrete measurements. This method is based on the numerical implementation of the Variational Inverse Model (VIM), which consists of a minimization of a cost function, allowing the choice of the analyzed field fitting at best the data sets. The problem is solved efficiently using a finite-element method. This statistical method is particularly suited to deal with irregularly-spaced observations, producing outputs on a regular grid. Initially created to work in a two-dimensional way, the software is now able to handle 3D or even 4D analysis, in order to easily produce ocean climatologies. These analyzes can easily be improved by taking advantage of the DIVA's ability to take topographic and dynamic constraints into account (coastal relief, prevailing wind impacting the advection,...). In DIVA, we assume errors on measurements are not correlated, which means we do not consider the effect of correlated observation errors on the analysis and we therefore use a diagonal observation error covariance matrix. However, the oceanographic data sets are generally clustered in space and time, thus introducing some correlation between observations. In order to determine the impact of such an approximation and provide strategies to mitigate its effects, we conducted several synthetic experiments with known correlation structure. Overall, the best results were obtained with a variant of the covariance inflation method. Finally, a new application of DIVA on satellite altimetry data will be presented : these data have particular space and time distributions, as they consist of repeated tracks (~10-35 days) of measurements with a distance lower than 10 km between two successive measurements in a given track. The tools designed to determine the analysis parameters were adapted to these specificities. Moreover, different weights were applied to measurements in order to

  19. A dádiva como princípio organizador da ciência

    Directory of Open Access Journals (Sweden)

    Marcos Barbosa De Oliveira

    2014-12-01

    Full Text Available Este artigo é fruto do trabalho num projeto de pesquisa mais amplo cujo tema são os processos de mercantilização pelos quais a ciência vem passando nas últimas décadas. A parte do projeto em que o artigo se insere diz respeito aos processos de empresariamento da ciência, que envolvem como componente principal a introdução de métodos quantitativos produtivistas de avaliação do trabalho científico. O objetivo do artigo é complementar a crítica a tais formas de avaliação com a sugestão de formas alternativas, inspiradas na concepção, devida a W. Hagstrom, da dádiva como o princípio organizador da ciência. A exposição divide-se em cinco seções: a primeira trata da dádiva em geral ou, em outras palavras, da dádiva como princípio organizador da sociedade; a segunda, da dádiva como princípio organizador da ciência; na terceira expõe-se um argumento a favor da concepção dadivosa, baseado em seu poder explicativo; na quarta, considerações sobre a proliferação de fraudes na ciência; e na quinta, um esquema conceitual como fundamento da transição do quantitativo para o qualitativo na avaliação acadêmica. A conclusão consiste em algumas considerações gerais, propostas como balizas para o movimento em prol de formas mais sensatas de organização das práticas científicas.

  20. Évolution spatio-temporelle des écosystèmes forestiers à pin D’Alep de L’Aurès oriental en Algérie

    Directory of Open Access Journals (Sweden)

    Kenza Garah

    2016-12-01

    Full Text Available CGarah, K., Chafai, C. and Bentouati, A. Évolution spatio-temporelle des écosystèmes forestiers à pin d’Alep de l'Aurès oriental en Algérie. 2016. Lebanese Science Journal, 17(2: 104-116. The present study falls within the framework of a characterization of the spatiotemporal dynamics of land use between 1987 and 2014 of a forest area located in eastern Aures. The method of diachronic comparison of satellite images was adopted. Results revealed profound regressive changes in aurasian forest during the last 27 years (from 1987 to 2014. This regression of vegetation cover is mainly localized in Aleppo pine forest stands which predominates the study area. The current degradation would be related to recent climate changes which have amplified the regression of certain forest ecosystems of the region.

  1. Interpolate with DIVA and view the products in OceanBrowser : what's up ?

    Science.gov (United States)

    Watelet, Sylvain; Barth, Alexander; Beckers, Jean-Marie; Troupin, Charles

    2017-04-01

    The Data-Interpolating Variational Analysis (DIVA) software is a statistical tool designed to reconstruct a continuous field from discrete measurements. This method is based on the numerical implementation of the Variational Inverse Model (VIM), which consists of a minimization of a cost function, allowing the choice of the analyzed field fitting at best the data sets without presenting unrealistic strong variations. The problem is solved efficiently using a finite-element method. This method, equivalent to the Optimal Interpolation, is particularly suited to deal with irregularly-spaced observations and produces outputs on a regular grid (2D, 3D or 4D). The results are stored in NetCDF files, the most widespread format in the earth sciences community. OceanBrowser is a web-service that allows one to visualize gridded fields on-line. Within the SeaDataNet and EMODNET (Chemical lot) projects, several national ocean data centers have created gridded climatologies of different ocean properties using the data analysis software DIVA. In order to give a common viewing service to those interpolated products, the GHER has developed OceanBrowser which is based on open standards from the Open Geospatial Consortium (OGC), in particular Web Map Service (WMS) and Web Feature Service (WFS). These standards define a protocol for describing, requesting and querying two-dimensional maps at a given depth and time. DIVA and OceanBrowser are both softwares tools which are continuously upgraded and distributed for free through frequent version releases. The development is funded by the EMODnet and SeaDataNet projects and include many discussions and feedback from the users community. Here, we present two recent major upgrades. First, we have implemented a "customization" of DIVA analyses following the sea bottom, using the bottom depth gradient as a new source of information. The weaker the slope of the bottom ocean, the higher the correlation length. This correlation length being

  2. Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine.

    Science.gov (United States)

    Bearson, Bradley L; Bearson, Shawn M D; Brunelle, Brian W; Bayles, Darrell O; Lee, In Soo; Kich, Jalusa D

    2017-05-01

    Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNA-Seq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Vaccination significantly reduced fever and interferon-gamma (IFNγ) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcine-derived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits.

  3. Web-based workflows to produce ocean climatologies using DIVA (Data-Interpolating Variational Analysis) and Jupyter notebooks

    Science.gov (United States)

    Barth, Alexander; Troupin, Charles; Watelet, Sylvain; Alvera-Azcarate, Aida; Beckers, Jean-Marie

    2017-04-01

    The analysis tool DIVA (Data-Interpolating Variational Analysis) is designed to generate gridded fields or climatologies from in situ observations. The tool DIVA minimizes a cost function to ensure that the analysed field is relatively close to the observations and conforms at the same time to a set of dynamical constraints. In particular, DIVA naturally decouples water bodies which are not directly connected and it uses a (potentially spatial varying) correlation length to describe over which length-scale the analysed variable is correlated. In addition, DIVA can also take ocean currents into account to introduce a preferential direction for the correlation. The SeaDataCloud project aims to facilitate the access and use of ocean in situ data from 45 national oceanographic data centres and marine data centres from 35 countries riparian to all European seas. A central aspect is to provide web-based virtual research environment, where scientists can easily access and explore the data sets through the SeaDataCloud infrastructure. For users familiar with programming languages like Julia and Python, Jupyter (acronym for Julia, Python and R) notebooks provide an exciting way to analyse and to interact with ocean data. Jupyter notebooks are made up of cells that can be run individually and can contain text, formulas or code fragment. A complete notebook explains how to go from input data and parameters to a result, in this case a gridded field obtained executing DIVA. This presentation discusses this new web-based workflow for generating climatologies using DIVA. It explores its new possibilities in particular, in terms of improved ease of use and reproducibility of the results. The integration in the infrastructure of EUDAT is also addressed.

  4. Trabalho imaterial, produção cultural colaborativa e economia da dádiva

    OpenAIRE

    Lima, Clóvis Ricardo Montenegro de; Pizarro, Daniella; Faustino, Elisangela; Dittrich, Maireli

    2009-01-01

    Neste artigo discutem-se as relações entre trabalho imaterial, produção cultural colaborativa e economia da dádiva na sociedade atual. O intelecto geral é relacionado com a inteligência coletiva das redes digitais. A produção colaborativa emerge como forma privilegiada do fazer artístico e cultural. Esta produção pode contribuir para promoção e preservação da diversidade cultural. A produção cultural colaborativa evidencia pluralismo econômico. A generalização do trabalho imaterial produz be...

  5. Trabalho imaterial, produção cultural colaborativa e economia da dádiva.

    OpenAIRE

    Lima, Clóvis Ricardo Montenegro de; Pizarro, Daniella; Faustino, Elisangela; Dittrich, Maireli

    2011-01-01

    Neste artigo discutem-se as relações entre trabalho imaterial, produção cultural colaborativa e economia da dádiva na sociedade atual. O intelecto geral é relacionado com a inteligência coletiva das redes digitais. A produção colaborativa emerge como forma privilegiada do fazer artístico e cultural. Esta produção pode contribuir para promoção e preservação da diversidade cultural. A produção cultural colaborativa evidencia pluralismo econômico. A generalização do trabalho imate...

  6. Trabalho imaterial, produção cultural colaborativa e economia da dádiva.

    OpenAIRE

    Lima,Clóvis Ricardo Montenegro de; Pizarro, Daniella; Faustino, Elisangela; Dittrich, Maireli

    2011-01-01

    Neste artigo discutem-se as relações entre trabalho imaterial, produção cultural colaborativa e economia da dádiva na sociedade atual. O intelecto geral é relacionado com a inteligência coletiva das redes digitais. A produção colaborativa emerge como forma privilegiada do fazer artístico e cultural. Esta produção pode contribuir para promoção e preservação da diversidade cultural. A produção cultural colaborativa evidencia pluralismo econômico. A generalização do trabalho imate...

  7. Strategies for differentiating infection in vaccinated animals (DIVA) for foot-and-mouth disease, classical swine fever and avian influenza

    DEFF Research Database (Denmark)

    Uttenthal, Åse; Parida, Satya; Rasmussen, Thomas Bruun;

    2010-01-01

    for the presence of infection. This literature review describes the current knowledge on the use of DIVA diagnostic strategies for three important transboundary animal diseases: foot-and-mouth disease in cloven-hoofed animals, classical swine fever in pigs and avian influenza in poultry....

  8. Development of a Luminex-Based DIVA Assay for Serological Detection of African Horse Sickness Virus in Horses.

    Science.gov (United States)

    Sánchez-Matamoros, A; Nieto-Pelegrín, E; Beck, C; Rivera-Arroyo, B; Lecollinet, S; Sailleau, C; Zientara, S; Sánchez-Vizcaíno, J M

    2016-08-01

    African horse sickness (AHS) is considered a fatal re-emergent vector-borne disease of horses. In the absence of any effective treatment for AHS, vaccination remains the most effective form of disease control. The new generation of vaccines, such as one based on purified, inactivated AHS virus (AHSV, serotype 4), which does not induce antibodies against non-structural protein 3 (NS3), enables the development of diagnostic methods that differentiate infected from vaccinated animals (DIVA assays). As detecting AHS in AHSV-free countries may lead to restrictions on international animal movements and thereby cause significant economic damage, these DIVA assays are crucial for reducing movement restrictions. In this article, we describe a Luminex-based multiplex assay for DIVA diagnosis of AHS, and we validate it in a duplex format to detect antibodies against structural protein 7 (VP7) and NS3 in serum samples from horses vaccinated with inactivated AHSV4 vaccine or infected with a live virus of the same serotype. Results of the Luminex-based assay for detecting anti-NS3 antibodies showed good positive correlation with results from an in-house enzyme-linked immunosorbent assay (ELISA). Thus, the Luminex-based technique described here may allow multiplex DIVA antibody detection in a single sample in less than 2 h, and it may prove adaptable for the development of robust, multiplex serological assays.

  9. Third generation DIVA vaccine towards classical swine fever virus. Efficacy in face of maternal immunity

    DEFF Research Database (Denmark)

    Rangelova, Desislava Yordanova

    General purpose and objectives Classical swine fever (CSF) is a highly contagious disease that causes huge economical losses and animal welfare concerns worldwide. Generally, vaccination is an effective and safe method to control the disease. Following vaccination the pig’s immune system develops...... antibodies that are significant part of the protection. However, vaccination with the only live attenuated vaccines existing on the market that contain a whole CSF virus (CSFV) with reduced infectivity, leads to production of an antibody response that does not differ from the antibody response developed......, will hamper the ability to proof a disease free status by serosurveillance, as all vaccinated piglets will be seropositive. This PhD-project is a part of an EU project (CSFV_goDIVA grant no 227003) that has been funded by the European Commission with a main goal to develop and test to a level of registration...

  10. Archaeometrical analysis of Neolithic pottery from the Divača region, Slovenia

    Directory of Open Access Journals (Sweden)

    Andreja Žibrat Gašparič

    2004-12-01

    Full Text Available The results of the mineralogical and chemical analyses of pottery from the Neolithic period from the Divača region are presented. Pottery samples from two rock shelters, i.e. Mala Triglavca and Trhlovca, were included in the analyses, as well as sediment samples from other rock shelters, caves and rivers around this area. The mineralogical and chemical composition of the ceramic is uniform in most of the samples; the differences between the clay pastes of the vessels are in the use of a tempering material, mostly calcite grains. The sediment samples, especially from the cave deposits, point to a local production of the Neolithic pottery on the Karst plateau.

  11. Inferring the biogeographic origins of inter-continental disjunct endemics using a Bayes-DIVA approach

    Institute of Scientific and Technical Information of China (English)

    AJ HARRIS; Jun WEN; Qiu-Yun (Jenny) XIANG

    2013-01-01

    The arcto-Tertiary relictual flora is comprised of many genera that occur non-contiguously in the temperate zones of eastern Asia,Europe,eastern North America,and westem North America.Within each distributional area,species are typically endemic and may thus be widely separated from closely related species within the other areas.It is widely accepted that this common pattern of distribution resulted from of the fragmentation of a once morecontinuous arcto-Tertiary forest.The historical biogeographic events leading to the present-day disjunction have often been investigated using a phylogenetic approach.Limitations to these previous studies have included phylogenetic uncertainty and uncertainty in ancestral range reconstructions.However,the recently described Bayes-DIVA method handles both types of uncertainty.Thus,we used Bayes-DIVA analysis to reconstruct the stem lineage distributions for 185 endemic lineages from 23 disjunct genera representing 17 vascular plant families.In particular,we asked whether endemic lineages within each of the four distributional areas more often evolved from (1) widespread ancestors,(2) ancestors dispersed from other areas,or (3) endemic ancestors.We also considered which of these three biogeographic mechanisms may best explain the origins of arcto-Tertiary disjunct endemics in the neotropics.Our results show that eastern Asian endemics more often evolved from endemic ancestors compared to endemics in Europe and eastern and western North America.Present-day endemic lineages in the latter areas more often arose from widespread ancestors.Our results also provide anecdotal evidence for the importance of dispersal in the biogeographic origins of arcto-Tertiary species endemic in the neotropics.

  12. Strong protection induced by an experimental DIVA subunit vaccine against bluetongue virus serotype 8 in cattle.

    Science.gov (United States)

    Anderson, Jenna; Hägglund, Sara; Bréard, Emmanuel; Riou, Mickaël; Zohari, Siamak; Comtet, Loic; Olofson, Ann-Sophie; Gélineau, Robert; Martin, Guillaume; Elvander, Marianne; Blomqvist, Gunilla; Zientara, Stéphan; Valarcher, Jean Francois

    2014-11-20

    Bluetongue virus (BTV) infections in ruminants pose a permanent agricultural threat since new serotypes are constantly emerging in new locations. Clinical disease is mainly observed in sheep, but cattle were unusually affected during an outbreak of BTV seroype 8 (BTV-8) in Europe. We previously developed an experimental vaccine based on recombinant viral protein 2 (VP2) of BTV-8 and non-structural proteins 1 (NS1) and NS2 of BTV-2, mixed with an immunostimulating complex (ISCOM)-matrix adjuvant. We demonstrated that bovine immune responses induced by this vaccine were as good or superior to those induced by a classic commercial inactivated vaccine. In this study, we evaluated the protective efficacy of the experimental vaccine in cattle and, based on the detection of VP7 antibodies, assessed its DIVA compliancy following virus challenge. Two groups of BTV-seronegative calves were subcutaneously immunized twice at a 3-week interval with the subunit vaccine (n=6) or with adjuvant alone (n=6). Following BTV-8 challenge 3 weeks after second immunization, controls developed viremia and fever associated with other mild clinical signs of bluetongue disease, whereas vaccinated animals were clinically and virologically protected. The vaccine-induced protection was likely mediated by high virus-neutralizing antibody titers directed against VP2 and perhaps by cellular responses to NS1 and NS2. T lymphocyte responses were cross-reactive between BTV-2 and BTV-8, suggesting that NS1 and NS2 may provide the basis of an adaptable vaccine that can be varied by using VP2 of different serotypes. The detection of different levels of VP7 antibodies in vaccinated animals and controls after challenge suggested a compliancy between the vaccine and the DIVA companion test. This BTV subunit vaccine is a promising candidate that should be further evaluated and developed to protect against different serotypes. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  13. A web interface for griding arbitrarily distributed in situ data based on Data-Interpolating Variational Analysis (Diva)

    Science.gov (United States)

    Barth, A.; Alvera-Azcárate, A.; Troupin, C.; Ouberdous, M.; Beckers, J.-M.

    2009-04-01

    Spatial interpolation of observations on a regular grid is a common task in many oceanographic disciplines (and geosciences in general). It is often used to create climatological maps for physical, biological or chemical parameters representing e.g. monthly or seasonally averaged fields. Since instantaneous observations can not be directly related to a field representing an average, simple spatial interpolation of observations is in general not acceptable. Diva (Data-Interpolating Variational Analysis) is an analysis tool which takes the error in the observations and the typical spatial scale of the underlying field into account. Barriers due to the coastline and the topography in general and also currents estimates (if available) are used to propagate the information of a given observation spatially. Diva is a command-line driven application written in Fortran and Shell Scripts. The observations and parameters are specified by the user using text files. The analyzed field and the expected error variance are returned as NetCDF files. This form of interaction with Diva is very similar to other high-performance codes and is a familiar approach for ocean modelers. However it represents a steep learning curve for oceanographers from other disciplines not familiar with command-line applications and programming. To make Diva easier to use, a web interface has been developed (http://gher-diva.phys.ulg.ac.be). Installation and compilation of Diva is therefore not required. The user can directly upload his/her data in ASCII format and enter several parameters for the analysis. The analyzed field, location of the observations, and the error mask are presented as different layers using the Web Map Service protocol. They are visualized in the browser using the Javascript library OpenLayers allowing the user to interact with layers (for example zooming and panning). Finally, the results can be downloaded as a NetCDF file, Matlab file (also readable in Octave, an open source

  14. Strategies for differentiating infection in vaccinated animals (DIVA) for foot-and-mouth disease, classical swine fever and avian influenza

    DEFF Research Database (Denmark)

    Uttenthal, Åse; Parida, Satya; Rasmussen, Thomas Bruun

    2010-01-01

    for the presence of infection. This literature review describes the current knowledge on the use of DIVA diagnostic strategies for three important transboundary animal diseases: foot-and-mouth disease in cloven-hoofed animals, classical swine fever in pigs and avian influenza in poultry.......The prophylactic use of vaccines against exotic viral infections in production animals is undertaken exclusively in regions where the disease concerned is endemic. In such areas, the infection pressure is very high and so, to assure optimal protection, the most efficient vaccines are used. However......, in areas considered to be free from these diseases and in which there is the possibility of only limited outbreaks, the use of Differentiation of Infected from Vaccinated Animals (DIVA) or marker vaccines allows for vaccination while still retaining the possibility of serological surveillance...

  15. RH diversity in Mali: characterization of a new haplotype RHD*DIVa/RHCE*ceTI(D2).

    Science.gov (United States)

    Ba, Alhassane; Beley, Sophie; Chiaroni, Jacques; Bailly, Pascal; Silvy, Monique

    2015-06-01

    Knowledge of RH variants in African populations is critical to improving transfusion safety in countries with populations of African ancestry and to providing valuable information and direction for future development of transfusion in Africa. The purpose of this report is to describe RH diversity in individuals from Mali. Blood samples collected from 147 individuals self-identified as Dogon and Fulani were analyzed for Rh antigens and alleles. The most common RHD allele variant was RHD*DAU0. Five predicted partial-D phenotypes were attributed to RHD*DAU3 or RHD*DIVa. Neither RHD*DAR nor RHD*DIIIa was found. Investigation of RHCE revealed three predicted partial-e antigens encoded by RHCE*ce(254G) in trans to RHCE*cE. Regarding C antigen, 28 Fulani typed as C+ and 16 of 28 harbored at least one RHCE*Ce-D(4)-ce, two being homozygous and predicted to show a rare RH:32,-46 phenotype. A new RHCE*ceTI with replacement of Exon 2 by RHD (RHCE*ceTI(D2)) was identified in Dogon and was identified by inheritance study to be in cis to RHD*DIVa. These samples typed C- with anti-C polyclonal antibody and monoclonal antibodies (MoAbs) MS24, P3X2551368+MS24, and MS273, but positive with anti-RhCe MoAb-BS58. The same pattern was observed in sample with RHD*DIVa/RHCE*ceTI. Our survey indicated an uneven distribution of RH variant alleles between Dogon and Fulani, suggesting that study in well-documented cohorts is warranted. A high incidence of predicted partial-C phenotype encoded by RHCE*Ce-D(4)-ce was found in Fulani. Further study will also be needed to clarify the clinical significance of the new DIVa/ceTI(D2) haplotype encoding partial D and variant ce antigens. © 2015 AABB.

  16. The it girl''s guide to becoming an Excel diva

    CERN Document Server

    Babaian, Ani

    2009-01-01

    Apply Excel daily and smooth out life''s wrinklesWho knew a spreadsheet could do as much for you as your favorite moisturizer? Become a Microsoft Excel Diva with this sassy guide and discover what hip IT Girls already know: smart is beautiful. Impress the heck out of everyone at work with your stylish reports. Do a budget and see exactly what you can spend on shoes. Get better organized. Who has time for dull technical manuals? Learn all of Microsoft Excel''s secrets in this girl-talk guide.Welcome to the party!Relax, refresh, and reward yourself at the end of each chapter with fun, stress-reducing chats, like why soy Chai lattes are better than regular lattes. Don''t miss these gems!* Meet Microsoft(r) Excel(r) 2007, the Louis Vuitton of spreadsheets* Join the conversation with these basic Excel terms* Learn the fine art of Excel formulas and functions* Dress up Excel data with SmartArt, WordArt, and other chic accessories* Build a shopping spree budget from scratch* Show off by adding Excel data into Word a...

  17. O trabalho individualizado: da venda à dádiva Individualized labor: from sale to gift

    Directory of Open Access Journals (Sweden)

    Hermano Roberto Thiry'Cherques

    2007-08-01

    Full Text Available Este artigo estabelece um paralelo entre a categoria da dádiva, tal como definida por Marcel Mauss, e as estruturas relacionais do trabalho. Com base em pesquisa realizada junto a 122 organizações públicas e privadas, de diversos setores da economia, foi possível identificar: a superação da estrutura do trabalho em equipe (teamwork e a prevalência de uma estrutura que privilegia o trabalho individualizado. A partir dessa constatação, o artigo discute a possibilidade de que o sistema de compra-venda do trabalho fragmentado esteja sendo substituído pelo sistema da dádiva-reconhecimento do trabalho individualizado. A pesquisa cujos resultados são comentados neste artigo indica que esse movimento não é fruto de uma evolução ou de uma circunstância momentânea, mas de uma mutação. Na tentativa de sobreviver às pressões do mercado, as organizações se encontraram na contingência de impor uma mudança radical na gestão de seus ativos. Como decorrência, a maneira como os recursos humanos são contratados, utilizados e descartados se alterou. A forma estrutural do trabalho-mercadoria vendido fragmentariamente se viu substituída pela estrutura em que o resultado do trabalho em lotes e frações integrais é transacionado contra recompensas que transcendem a simples remuneração.This article establishes a parallel between the theory of gift exchange, as expounded by Marcel Mauss, and structural relationships in the workplace. Based on research conducted in 122 public and private organizations, in various sectors of the economy, it was possible to identify: the obsolescence of the teamwork structure and the prevalence of a structure that favors individualized labor. Based on this premise, the article discusses the possibility that the system of purchase-sale of fragmented labor is in the process of being replaced by the system of gift-recognition of individualized labor. The results of a research discussed in this article reveal

  18. Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

    Science.gov (United States)

    2011-01-01

    Background Protection of pigs by vaccination against Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is hampered by the presence of 15 different serotypes. A DIVA subunit vaccine comprised of detergent-released proteins from A. pleuropneumoniae serotypes 1, 2 and 5 has been developed and shown to protect pigs from clinical symptoms upon homologous and heterologous challenge. This vaccine has not been characterized in-depth so far. Thus we performed i) mass spectrometry in order to identify the exact protein content of the vaccine and ii) cross-serotype 2-D immunoblotting in order to discover cross-reactive antigens. By these approaches we expected to gain results enabling us to argue about the reasons for the efficacy of the analyzed vaccine. Results We identified 75 different proteins in the vaccine. Using the PSORTb algorithm these proteins were classified according to their cellular localization. Highly enriched proteins are outer membrane-associated lipoproteins like OmlA and TbpB, integral outer membrane proteins like FrpB, TbpA, OmpA1, OmpA2, HgbA and OmpP2, and secreted Apx toxins. The subunit vaccine also contained large amounts of the ApxIVA toxin so far thought to be expressed only during infection. Applying two-dimensional difference gel electrophoresis (2-D DIGE) we showed different isoforms and variations in expression levels of several proteins among the strains used for vaccine production. For detection of cross-reactive antigens we used detergent released proteins of serotype 7. Sera of pigs vaccinated with the detergent-released proteins of serotypes 1, 2, and 5 detected seven different proteins of serotype 7, and convalescent sera of pigs surviving experimental infection with serotype 7 reacted with 13 different proteins of the detergent-released proteins of A. pleuropneumoniae serotypes 1, 2, and 5. Conclusions A detergent extraction-based subunit vaccine of A. pleuropneumoniae was characterized by mass

  19. Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

    Directory of Open Access Journals (Sweden)

    Maas Alexander

    2011-04-01

    Full Text Available Abstract Background Protection of pigs by vaccination against Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is hampered by the presence of 15 different serotypes. A DIVA subunit vaccine comprised of detergent-released proteins from A. pleuropneumoniae serotypes 1, 2 and 5 has been developed and shown to protect pigs from clinical symptoms upon homologous and heterologous challenge. This vaccine has not been characterized in-depth so far. Thus we performed i mass spectrometry in order to identify the exact protein content of the vaccine and ii cross-serotype 2-D immunoblotting in order to discover cross-reactive antigens. By these approaches we expected to gain results enabling us to argue about the reasons for the efficacy of the analyzed vaccine. Results We identified 75 different proteins in the vaccine. Using the PSORTb algorithm these proteins were classified according to their cellular localization. Highly enriched proteins are outer membrane-associated lipoproteins like OmlA and TbpB, integral outer membrane proteins like FrpB, TbpA, OmpA1, OmpA2, HgbA and OmpP2, and secreted Apx toxins. The subunit vaccine also contained large amounts of the ApxIVA toxin so far thought to be expressed only during infection. Applying two-dimensional difference gel electrophoresis (2-D DIGE we showed different isoforms and variations in expression levels of several proteins among the strains used for vaccine production. For detection of cross-reactive antigens we used detergent released proteins of serotype 7. Sera of pigs vaccinated with the detergent-released proteins of serotypes 1, 2, and 5 detected seven different proteins of serotype 7, and convalescent sera of pigs surviving experimental infection with serotype 7 reacted with 13 different proteins of the detergent-released proteins of A. pleuropneumoniae serotypes 1, 2, and 5. Conclusions A detergent extraction-based subunit vaccine of A. pleuropneumoniae was

  20. Evaluation of a commercial ELISA kit (IDEXX to differentiate AI virus-infected poultry from AI-vaccinated poultry (DIVA

    Directory of Open Access Journals (Sweden)

    F Talazadeh

    2014-06-01

    Full Text Available Vaccination programs for the control of avian influenza (AI in poultry have limitations due to the problem of differentiating between vaccinated and virus-infected birds. The use of AI vaccination in poultry would have greater worldwide acceptance if reliable tests that clearly discriminated between naturally infected and vaccinated-only animals (DIVA were available. In this study, to differentiate avian influenza virus (AIV-infected chickens vs. chickens immunized with inactivated avian influenza virus, a commercial ELISA Kit (IDEXX was used. Using HI as the reference method, the sensitivity of the commercial ELISA kit was 100% and its specificity was 100%. In the present study, we demonstrated that, 3 weeks after infection or vaccination, sera from both infected and vaccinated groups were tested positive and the difference in mean optical density between vaccinated and challenged birds, as detected by a commercial ELISA kit (IDEXX, was very small. Therefore, this assay cannot distinguish infected from vaccinated poultry.

  1. Recombinant adenovirus expressing the haemagglutinin of Peste des petits ruminants virus (PPRV) protects goats against challenge with pathogenic virus; a DIVA vaccine for PPR.

    Science.gov (United States)

    Herbert, Rebecca; Baron, Jana; Batten, Carrie; Baron, Michael; Taylor, Geraldine

    2014-02-26

    Peste des petits ruminants virus (PPRV) is a morbillivirus that can cause severe disease in sheep and goats, characterised by pyrexia, pneumo-enteritis, and gastritis. The socio-economic burden of the disease is increasing in underdeveloped countries, with poor livestock keepers being affected the most. Current vaccines consist of cell-culture attenuated strains of PPRV, which induce a similar antibody profile to that induced by natural infection. Generation of a vaccine that enables differentiation of infected from vaccinated animals (DIVA) would benefit PPR control and eradication programmes, particularly in the later stages of an eradication campaign and for countries where the disease is not endemic. In order to create a vaccine that would enable infected animals to be distinguished from vaccinated ones (DIVA vaccine), we have evaluated the immunogenicity of recombinant fowlpox (FP) and replication-defective recombinant human adenovirus 5 (Ad), expressing PPRV F and H proteins, in goats. The Ad constructs induced higher levels of virus-specific and neutralising antibodies, and primed greater numbers of CD8+ T cells than the FP-vectored vaccines. Importantly, a single dose of Ad-H, with or without the addition of Ad expressing ovine granulocyte macrophage colony-stimulating factor and/or ovine interleukin-2, not only induced strong antibody and cell-mediated immunity but also completely protected goats against challenge with virulent PPRV, 4 months after vaccination. Replication-defective Ad-H therefore offers the possibility of an effective DIVA vaccine.

  2. Development of the A-DIVA Scale: A Clinical Predictive Scale to Identify Difficult Intravenous Access in Adult Patients Based on Clinical Observations.

    Science.gov (United States)

    van Loon, Fredericus H J; Puijn, Lisette A P M; Houterman, Saskia; Bouwman, Arthur R A

    2016-04-01

    Placement of a peripheral intravenous catheter is a routine procedure in clinical practice, but failure of intravenous cannulation regularly occurs. An accurate and reliable predictive scale for difficult venous access creates the possibility to use other techniques in an earlier time frame. We aimed to develop a predictive scale to identify adult patients with a difficult intravenous access prospectively: the A-DIVA scale. This prospective, observational, cross-sectional cohort study was conducted between January 2014 and January 2015, and performed at the department of anesthesiology of the Catharina Hospital (Eindhoven, The Netherlands). Patients 18 years or older were eligible if scheduled for any surgical procedure, regardless ASA classification, demographics, and medical history. Experienced and certified anesthesiologists and nurse anesthetists routinely obtained peripheral intravenous access. Cannulation was performed regarding standards for care. A failed peripheral intravenous cannulation on the first attempt was the outcome of interest. A population-based sample of 1063 patients was included. Failure of intravenous cannulation was observed in 182/1063 patients (17%). Five variables were associated with a failed first attempt of peripheral intravenous cannulation: palpability of the target vein (OR = 4.94, 95% CI [2.85-8.56]; P < 0.001), visibility of the target vein (OR = 3.63, 95% CI [2.09-6.32]; P < 0.001), a history of difficult peripheral intravenous cannulation (OR = 3.86, 95% CI [2.39-6.25]; P < 0.001), an unplanned indication for surgery (OR = 4.86, 95% CI [2.92-8.07]; P < 0.001), and the vein diameter of at most 2 millimeters (OR = 3.37, 95% CI [2.12-5.36]; P < 0.001). The scoring system was applied in 3 risk groups: 36/788 patients (5%) suffered from a failed first attempt in the low-risk group (A-DIVA score 0 or 1), whereas the medium (A-DIVA score 2 or 3) and high-risk group (A-DIVA score 4 plus

  3. Supra-complete surgery via dual intraoperative visualization approach (DiVA) prolongs patient survival in glioblastoma

    Science.gov (United States)

    Eyüpoglu, Ilker Y.; Hore, Nirjhar; Merkel, Andreas; Buslei, Rolf; Buchfelder, Michael; Savaskan, Nicolai

    2016-01-01

    Safe and complete resection represents the first step in the treatment of glioblastomas and is mandatory in increasing the effectiveness of adjuvant therapy to prolong overall survival. With gross total resection currently limited in extent to MRI contrast enhancing areas, the extent to which supra-complete resection beyond obvious contrast enhancement could have impact on overall survival remains unclear. DiVA (dual intraoperative visualization approach) redefines gross total resection as currently accepted by enabling for the first time supra-complete surgery without compromising patient safety. This approach exploits the advantages of two already accepted surgical techniques combining intraoperative MRI with integrated functional neuronavigation and 5-ALA by integrating them into a single surgical approach. We investigated whether this technique has impact on overall outcome in GBM patients. 105 patients with GBM were included. We achieved complete resection with intraoperative MRI alone according to current best-practice in glioma surgery in 75 patients. 30 patients received surgery with supra-complete resection. The control arm showed a median life expectancy of 14 months, reflecting current standards-of-care and outcome. In contrast, patients receiving supra-complete surgery displayed significant increase in median survival time to 18.5 months with overall survival time correlating directly with extent of supra-complete resection. This extension of overall survival did not come at the cost of neurological deterioration. We show for the first time that supra-complete glioma surgery leads to significant prolongation of overall survival time in GBM patients. PMID:27036027

  4. Supra-complete surgery via dual intraoperative visualization approach (DiVA) prolongs patient survival in glioblastoma.

    Science.gov (United States)

    Eyüpoglu, Ilker Y; Hore, Nirjhar; Merkel, Andreas; Buslei, Rolf; Buchfelder, Michael; Savaskan, Nicolai

    2016-05-01

    Safe and complete resection represents the first step in the treatment of glioblastomas and is mandatory in increasing the effectiveness of adjuvant therapy to prolong overall survival. With gross total resection currently limited in extent to MRI contrast enhancing areas, the extent to which supra-complete resection beyond obvious contrast enhancement could have impact on overall survival remains unclear. DiVA (dual intraoperative visualization approach) redefines gross total resection as currently accepted by enabling for the first time supra-complete surgery without compromising patient safety. This approach exploits the advantages of two already accepted surgical techniques combining intraoperative MRI with integrated functional neuronavigation and 5-ALA by integrating them into a single surgical approach. We investigated whether this technique has impact on overall outcome in GBM patients. 105 patients with GBM were included. We achieved complete resection with intraoperative MRI alone according to current best-practice in glioma surgery in 75 patients. 30 patients received surgery with supra-complete resection. The control arm showed a median life expectancy of 14 months, reflecting current standards-of-care and outcome. In contrast, patients receiving supra-complete surgery displayed significant increase in median survival time to 18.5 months with overall survival time correlating directly with extent of supra-complete resection. This extension of overall survival did not come at the cost of neurological deterioration. We show for the first time that supra-complete glioma surgery leads to significant prolongation of overall survival time in GBM patients.

  5. Potential Distribution of Chalara fraxinea in China Predicted by MaxEnt and DIVA-GIS%基于MaxEnt和DIVA-GIS的白蜡枯梢病菌在中国的潜在适生区分析

    Institute of Scientific and Technical Information of China (English)

    罗志萍; 潘绪斌; 王聪; 李来梅

    2015-01-01

    为了开展危险性林术病害白蜡枯梢病的风险分析和检疫工作,研究对白蜡枯梢病菌在中国的潜在适生区进行了预测.利用MaxEnt软件和DIVA-GIS数据库,选择19个环境因子变量和2.5 min的空间分辨率,并对预测结果的可靠性进行ROC曲线法分析评价,同时采用Jackknife刀切法分析了各环境变量对预测结果的影响.结果表明:该病菌在我国局部地区有一定适生风险;根据得到的潜在适生区预测图,白蜡枯梢病菌在中国的高度适生区有台湾中部,中度适生区有西藏西南边陲林区、湖北神农架和宜昌三峡林区、湖南武陵山和张家界林区、广东、广西、重庆、贵州、江西、福建的局部等地;各环境因子贡献率分析表明最冷季降水量对白蜡枯梢病菌的适生区分布影响最大,研究预测结果AUC值为0.932,表明预测结果可信度高.

  6. Trabalho imaterial, produção cultural colaborativa e economia da dádiva | Immaterial labour, collaborative cultural production and the economy of the gift

    Directory of Open Access Journals (Sweden)

    Clóvis Ricardo Montenegro de Lima

    2009-09-01

    Full Text Available Resumo Neste artigo discutem-se as relações entre trabalho imaterial, produção cultural colaborativa e  economia da dádiva na sociedade atual. O intelecto geral é relacionado com a inteligência coletiva das redes digitais. A produção colaborativa emerge como forma privilegiada do fazer artístico e cultural. Esta produção pode contribuir para promoção e preservação da diversidade cultural. A produção cultural colaborativa evidencia pluralismo econômico. A generalização do trabalho imaterial produz bens comuns. O comum cria a versão pós-industrial e de alta tecnologia da economia da dádiva Conclui-se que a cultura colaborativa tem grande potencial para promover diversidade cultural e economia da generosidade. Palavras-chave trabalho imaterial; produção colaborativa; cultura; economia da dádiva; produção cultural colaborativa. Abstract This article discusses the relationship between immaterial labor, collaborative cultural production and gift economy in society today. The general intellect is related to the collective intelligence of digital networks. The collaborative production emerges as the preferred way to artistic and cultural. This production may contribute to promotion and preservation of cultural diversity. The collaborative cultural production highlights pluralism economics. The spread of immaterial labor produces common assets. The common creates a post-industrial and high-tech version of the gift economy. It is concluded that the collaborative culture has great potential to promote cultural diversity and economy of generosity. Keywords immaterial labor; collaborative production; culture; gift economy; collaborative cultural production.

  7. Development of a competitive ELISA for NS3 antibodies as DIVA test accompanying the novel Disabled Infectious Single Animal (DISA) vaccine for Bluetongue.

    Science.gov (United States)

    Tacken, Mirriam G J; Daus, Franz J; Feenstra, Femke; van Gennip, René G P; van Rijn, Piet A

    2015-10-13

    Recently, we have developed a novel vaccine for Bluetongue named BT Disabled Infectious Single Animal (DISA) vaccine. Due to the lack of non-essential NS3/NS3a protein, BT DISA vaccine is a replicating vaccine, but without the inherent risks of live-attenuated vaccines, such as residual virulence or reversion to virulence by mutations, reassortment with field virus, horizontal spread by vectors and vertical transmission. The immune response induced by BT DISA vaccines is rapidly induced, highly protective and serotype specific which is dependent on the immunodominant and serotype determining VP2 protein. The BT DISA vaccine platform provides the replacement of exclusively VP2 from different serotypes in order to safely formulate multivalent cocktail vaccines. The lack of NS3/NS3a directed antibodies by BT DISA vaccination enables differentiation of infected from vaccinated animals (DIVA principle). A highly conserved immunogenic site corresponding to the late domain was mapped in the N-terminal region of NS3. We here established an NS3-specific competitive ELISA (NS3 cELISA) as serological DIVA test accompanying BT DISA vaccines. To this end, NS3 protein missing putative transmembrane regions was produced in large amounts in bacteria and used as antigen in the NS3 cELISA which was investigated with a variety of sera. The NS3 cELISA displayed a high sensitivity and specificity similar to the commercially available VP7-specific cELISA. Results of previously performed vaccination-challenge trials with BT DISA vaccines clearly demonstrate the DIVA system based on the NS3 cELISA and BT vaccine free of NS3 protein.

  8. Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study

    Directory of Open Access Journals (Sweden)

    A.G. Pose

    2015-09-01

    Full Text Available In this study we demonstrated that the vaccine candidate against avian influenza virus H5N1 based on the hemagglutinin H5 (HA fused to the chicken CD154 (HACD can also be used for differentiating infected from vaccinated animals (DIVA. As the strategy of DIVA requires at least two proteins, we obtained a variant of the nucleoprotein (NP49-375 in E. coli. After its purification by IMAC, the competence of the proteins NP49-375 and HACD as coating antigens in indirect ELISA assays were tested by using the sera of chickens immunized with the proteins HA and HACD and the reference sera from several avian influenza subtypes. Together with these sera, the sera from different species of birds and the sera of chickens infected with other avian viral diseases were analyzed by competition ELISA assays coated with the proteins NP49-375 and HACD. The results showed that the segment CD154 in the chimeric protein HACD did not interfere with the recognition of the molecule HA by its specific antibodies. Also, we observed variable detection levels when the reference sera were analyzed in the ELISA plates coated with the protein NP49-375. Moreover, only the antibodies of the reference serum subtype H5 were detected in the ELISA plates coated with the protein HACD. The competition ELISA assays showed percentages of inhibition of 88-91% for the positives sera and less than 20% for the negative sera. We fixed the cut-off value of these assays at 25%. No antibody detection was observed in the sera from different species of birds or the sera of chickens infected with other avian viral diseases. This study supported the fact that the ELISA assays using the proteins NP49-375 and HACD could be valuable tools for avian influenza surveillance and as a strategy of DIVA for counteracting the highly pathogenic avian influenza virus H5N1 outbreaks.

  9. Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study

    Science.gov (United States)

    Pose, A.G.; Rodríguez, E.S.; Méndez, A.C.; Gómez, J.N.; Redondo, A.V.; Rodríguez, E.R.; Ramos, E.M.G.; Gutiérrez, A.Á.; Moltó, M.P.R.; Roche, D.G.; Ugalde, Y.S.; López, A.M.

    2015-01-01

    In this study we demonstrated that the vaccine candidate against avian influenza virus H5N1 based on the hemagglutinin H5 (HA) fused to the chicken CD154 (HACD) can also be used for differentiating infected from vaccinated animals (DIVA). As the strategy of DIVA requires at least two proteins, we obtained a variant of the nucleoprotein (NP49-375) in E. coli. After its purification by IMAC, the competence of the proteins NP49-375 and HACD as coating antigens in indirect ELISA assays were tested by using the sera of chickens immunized with the proteins HA and HACD and the reference sera from several avian influenza subtypes. Together with these sera, the sera from different species of birds and the sera of chickens infected with other avian viral diseases were analyzed by competition ELISA assays coated with the proteins NP49-375 and HACD. The results showed that the segment CD154 in the chimeric protein HACD did not interfere with the recognition of the molecule HA by its specific antibodies. Also, we observed variable detection levels when the reference sera were analyzed in the ELISA plates coated with the protein NP49-375. Moreover, only the antibodies of the reference serum subtype H5 were detected in the ELISA plates coated with the protein HACD. The competition ELISA assays showed percentages of inhibition of 88-91% for the positives sera and less than 20% for the negative sera. We fixed the cut-off value of these assays at 25%. No antibody detection was observed in the sera from different species of birds or the sera of chickens infected with other avian viral diseases. This study supported the fact that the ELISA assays using the proteins NP49-375 and HACD could be valuable tools for avian influenza surveillance and as a strategy of DIVA for counteracting the highly pathogenic avian influenza virus H5N1 outbreaks. PMID:26623380

  10. Research on a Method of EEG Signal Processing Based on DIVA Model%基于DIVA模型的脑电信号处理方法研究

    Institute of Scientific and Technical Information of China (English)

    张少白; 王勇; 刘友谊

    2016-01-01

    DIVA ( Directions Into Velocities of Articulators) is an adaptive neural network model which is used to control the movement of analog vocal tract for generating the words,syllables or phonemes. The input signal that DIVA model depends on is the EEG ( Electro-EncephaloGram) signal collected from the human brain. However,EEG data collected through non-invasive brain-computer interface shows low resolution and large interference in the research of Chinese Neuralynx System. A constraint processing method for the EEG sig-nal based on DIVA model is proposed. Firstly,it uses ICA ( Independent Component Analysis) to exclude the original noise in the signal and extract the effective ERP ( Event-Related Potentials) ingredient. Then,on condition of spatial information of points which are activa-ted by fMRI ( functional Magnetic Resonance Imaging) data,it locates ERP ingredient precisely. Finally,through processing and analy-zing experimental data and simulating activated brain regions of the subjects,the result verifies correctness and validity of the method.%DIVA( Directions Into of Articulators)模型是一种为了生成单词、音节或者音素而控制模拟声道运动的自适应神经网络模型,其依赖的输入信号是从人体大脑中采集到的脑电信号。针对汉语神经分析系统研究中非侵入式脑机接口采集到的脑电数据存在的分辨率低、干扰大的问题,文中提出一种基于DIVA模型对脑电信号进行约束处理的方法。首先利用独立分量分析方法剔除原始信号中的噪声,提取有效事件相关电位( Event-Related Potentials,ERP)成分;然后以模拟生成的功能性磁共振成像(functional Magnetic Resonance Imaging,fMRI)数据的激活点的空间信息作为限制条件,对提取出的ERP成分进行精确定位。通过对实验数据进行分析处理并模拟受试者的激活脑区,验证了所提方法的正确性和有效性。

  11. Protective efficacy of crude virus-like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy.

    Science.gov (United States)

    Park, Jae-Keun; Lee, Dong-Hun; Youn, Ha-Na; Kim, Myeong-Seob; Lee, Yu-Na; Yuk, Seong-Su; Lim, Tae-Hyun; Jang, Jun-Hyuk; Kwon, Jung-Hoon; Kim, Byoung-Yoon; Kang, Sang-Moo; Seong, Baik-Lin; Lee, Joong-Bok; Park, Seung-Yong; Choi, In-Soo; Song, Chang-Seon

    2013-05-01

    Currently, Asian lineage highly pathogenic avian influenza (HPAI) H5N1 has become widespread across continents. These viruses are persistently circulating among poultry populations in endemic regions, causing huge economic losses, and raising concerns about an H5N1 pandemic. To control HPAI H5N1, effective vaccines for poultry are urgently needed. In this study, we developed HPAI virus-like particle (VLP) vaccine as a candidate poultry vaccine and evaluated its protective efficacy and possible application for differentiating infected from vaccinated animals (DIVA). Specific pathogen-free chickens received a single injection of HPAI H5N1 VLP vaccine generated using baculovirus expression vector system. Immunogenicity of VLP vaccines was determined using hemagglutination inhibition (HI), neuraminidase inhibition (NI), and ELISA test. Challenge study was performed to evaluate efficacy of VLP vaccines. A single immunization with HPAI H5N1 VLP vaccine induced high levels of HI and NI antibodies and protected chickens from a lethal challenge of wild-type HPAI H5N1 virus. Viral excretion from the vaccinated and challenged group was strongly reduced compared with a mock-vaccinated control group. Furthermore, we were able to differentiate VLP-vaccinated chickens from vaccinated and then infected chickens with a commercial ELISA test kit, which offers a promising strategy for the application of DIVA concept. © 2012 Blackwell Publishing Ltd.

  12. Intermediate rough Brucella abortus S19Δper mutant is DIVA enable, safe to pregnant guinea pigs and confers protection to mice.

    Science.gov (United States)

    Lalsiamthara, Jonathan; Gogia, Neha; Goswami, Tapas K; Singh, R K; Chaudhuri, Pallab

    2015-05-21

    Brucella abortus S19 is a smooth strain used as live vaccine against bovine brucellosis. Smooth lipopolysaccharide (LPS) is responsible for its residual virulence and serological interference. Rough mutants defective of LPS are more attenuated but confers lower level of protection. We describe a modified B. abortus S19 strain, named as S19Δper, which exhibits intermediate rough phenotype with residual O-polysaccharide (OPS). Deletion of perosamine synthetase gene resulted in substantial attenuation of S19Δper mutant without affecting immunogenic properties. It mounted strong immune response in Swiss albino mice and conferred protection similar to S19 vaccine. Immunized mice produced higher levels of IFN-γ, IgG2a and thus has immune response inclined towards Th1 cell mediated immunity. Sera from immunized animals did not show agglutination reaction with RBPT antigen and thus could serve as DIVA (Differentiating Infected from Vaccinated Animals) vaccine. S19Δper mutant displayed more susceptibility to serum complement mediated killing and sensitivity to polymyxin B. Pregnant guinea pigs injected with S19Δper mutant completed full term of pregnancy and did not cause abortion, still birth or birth of weak offspring. S19Δper mutant with intermediate rough phenotype displayed remarkable resemblance to S19 vaccine strain with improved properties of safety, immunogenicity and DIVA capability for control of bovine brucellosis.

  13. Global coastal wetland change under sea-level rise and related stresses: The DIVA Wetland Change Model

    Science.gov (United States)

    Spencer, Thomas; Schuerch, Mark; Nicholls, Robert J.; Hinkel, Jochen; Lincke, Daniel; Vafeidis, A. T.; Reef, Ruth; McFadden, Loraine; Brown, Sally

    2016-04-01

    The Dynamic Interactive Vulnerability Assessment Wetland Change Model (DIVA_WCM) comprises a dataset of contemporary global coastal wetland stocks (estimated at 756 × 103 km2 (in 2011)), mapped to a one-dimensional global database, and a model of the macro-scale controls on wetland response to sea-level rise. Three key drivers of wetland response to sea-level rise are considered: 1) rate of sea-level rise relative to tidal range; 2) lateral accommodation space; and 3) sediment supply. The model is tuned by expert knowledge, parameterised with quantitative data where possible, and validated against mapping associated with two large-scale mangrove and saltmarsh vulnerability studies. It is applied across 12,148 coastal segments (mean length 85 km) to the year 2100. The model provides better-informed macro-scale projections of likely patterns of future coastal wetland losses across a range of sea-level rise scenarios and varying assumptions about the construction of coastal dikes to prevent sea flooding (as dikes limit lateral accommodation space and cause coastal squeeze). With 50 cm of sea-level rise by 2100, the model predicts a loss of 46-59% of global coastal wetland stocks. A global coastal wetland loss of 78% is estimated under high sea-level rise (110 cm by 2100) accompanied by maximum dike construction. The primary driver for high vulnerability of coastal wetlands to sea-level rise is coastal squeeze, a consequence of long-term coastal protection strategies. Under low sea-level rise (29 cm by 2100) losses do not exceed ca. 50% of the total stock, even for the same adverse dike construction assumptions. The model results confirm that the widespread paradigm that wetlands subject to a micro-tidal regime are likely to be more vulnerable to loss than macro-tidal environments. Countering these potential losses will require both climate mitigation (a global response) to minimise sea-level rise and maximisation of accommodation space and sediment supply (a regional

  14. 基于DIVA模型的语音-映射单元自动获取%Automatic acquisition of speech sound-target cells based on DIVA model

    Institute of Scientific and Technical Information of China (English)

    张少白; 刘欣

    2013-01-01

    针对DIVA模型中存在的“感知能力与语音生成技巧发育不平衡”问题,提出了一种自动获取语音-映射单元的方法。该方法将人耳模拟为一个具有不同带宽的并联带通滤波器组,分别与模型中21维度的听觉存储空间相关联,对不同听觉的不同反应,分别考虑其频带的屏蔽效应、听觉响度与频率的关系。在读取语音输入信号的过程中,模型能较好地获得初始听觉表示,其方式与婴儿咿呀学语的过程基本一致。仿真实验表明,通过边界定义、相似性比较以及搜索更新等步骤,此方法能很好地进行初始输入模式的自组织匹配,并最终使DIVA模型更具语音获取的自然特性。%Contraposing the shortage of Directions Into Velocities of Articulators ( DIVA) model about“infants per-ceptual abilities do develop faster at first than their speech production skills”, the paper presents an automatic ac-quisition method of speech sound-target cells. The method simulates the human ear as a parallel band-pass filter group with different bandwidth and associates respectively;the filter with the 21-dimensional storage space of audi-tory sense in DIVA model. This method was done in order for different auditory reactions, the shielding effect of fre-quency band, sound loudness, and frequency relation could be considered respectively for this study. In the process of reading the input signal of speech, the model can acquire good initial hearing and the process is consistent with baby's babble. The simulation results show that through boundary definition, similarity comparison, searching and updates and so on, the method has nicer self-organized pattern matching effect for initial input, which makes the DIVA model a more natural characteristic regarding speech acquisition.

  15. Violência simbólica e redes sociais no facebook: o caso da fanpage “Diva Depressão”

    Directory of Open Access Journals (Sweden)

    Raquel Recuero

    2013-12-01

    Full Text Available O presente artigo foca a violência simbólica e sua propagação através do discurso em sites de rede social. O objetivo é discutir os mecanismos através dos quais o estigma social é exposto e reforçado no Facebook, através do conteúdo publicado por fanpages humorísticas. Para trazer evidências para esta discussão, apresentamos dados de uma fanpage popular, “Diva Depressão” e analisamos três postagens e seus comentários através da Análise do Discurso Mediado pelo Computador (CMDA. Os resultados apontam para evidências de que tanto o conteúdo das páginas quanto as interações (comentários, curtidas e compartilhamentos reforçam e naturalizam o estigma.

  16. Study of DIVA Model Movement Sensory Systems Latency Problems%DIVA模型中运动感觉系统传输延迟问题的研究

    Institute of Scientific and Technical Information of China (English)

    高丽琴; 张少白

    2012-01-01

    The problem of the speech acquisition and production control needs to be solved for the robot voice system. Earlier versions of the DIVA (Directions Into Velocities of Articulators) model does not have the nerve physiology control function. To make the robot simulate the human sound and solve the problem of the feedback realistic delays in the sensory system,it adopts the strategy that feedforward control mechanism supplements feedback control mechanism. Aiming at the network state disturbance caused by parameters changes during the neural network learning process,it does related emulation experiments. Experimental results show that this method is effective and gives good robustness.%语音生成与获取的控制问题,是机器人发声系统急需解决的问题.早期的DIVA( Directions Into Velocities of Articulators)模型并不完全具备神经生理学意义上的控制功能.为使机器人模拟声道发出类似人类语言的声音,解决好运动感觉系统中感官反馈时间延迟的问题,文中通过采用前馈控制机制补充反馈控制的策略,来处理语音运动控制方面的延迟问题,并且最后针对模型神经网络学习过程中延迟参数变化引起的网络状态扰动问题,进行了颚扰动补偿仿真实验.实验结果表明,其给出的方法行之有效且鲁棒性良好.

  17. Um trabalho a troco de nada? A ação de uma comunidade de hackers à luz da teoria da dádiva

    Directory of Open Access Journals (Sweden)

    GENAUTO CARVALHO DE FRANÇA FILHO

    2014-08-01

    Full Text Available Mais do que apontar possíveis respostas para um "enigma" contemporâneo, este artigo discute as especificidades da dinâmica de trabalho dos hackers, no processo de produção não-contratual e colaborativo, presente na comunidade on-line de desenvolvimento de softwares do Projeto GNOME (GNU Network Object Model Environment. A partir de uma imersão netnográfica de mais de um ano, analisou-se a organização do trabalho que dá vida ao processo de produção empreendido por mais de 300 hackers e colaboradores de todos os cinco continentes do planeta. Mais especificamente, buscou-se compreender a natureza do trabalho adotado pelos hackers ao longo do desenvolvimento e distribuição de softwares nessa comunidade online de abrangência internacional. Como resultado, constatou-se a presença no Projeto GNOME de um tipo de engajamento não-contratual, associado a uma forma de trabalho e circulação de bens que se difere completamente de organizações ligadas à esfera do mercado ou do Estado, mas que consegue, por meio da internet, viabilizar um processo internacional de desenvolvimento de software de alta complexidade. Em outras palavras, foi possível verificar por meio da dinâmica social empreendida no seio dessa comunidade online, a emergência, nos liames digitais da internet, de uma nova expressão da dádiva moderna: um sistema de dádiva mediada por computador, tanto na essência como no modo de funcionamento e organização do trabalho.

  18. Effects of scale and input data on assessing the future impacts of coastal flooding: An application of DIVA for the Emilia-Romagna coast.

    Directory of Open Access Journals (Sweden)

    Claudia eWolff

    2016-04-01

    Full Text Available This paper assess sea-level rise related coastal flood impacts for Emilia-Romagna (Italy using the Dynamic Interactive Vulnerability Assessment (DIVA modeling framework and investigate the sensitivity of the model to four uncertainty dimensions, namely (1 elevation, (2 population (3 vertical land movement (4 scale and resolution of assessment. A one-driver-at-a-time sensitivity approach is used in order to explore and quantify the effects of uncertainties in input data and assessment scale on model outputs. Of particular interest is the sensitivity of flood risk estimates when using datasets of different resolution. The change in assessment scale is implemented through the use of a more detailed digital coastline and input data for the coastline segmentation process. This change leads to a 35-fold increase in the number of coastal segments and in a more realistic spatial representation of coastal flood impacts for the Emilia-Romagna coast. Furthermore, the coastline length increases by 43%, considerably influencing adaptation costs (construction of dikes. With respect to input data our results show that by the end of the century coastal flood impacts are more sensitive to variations in elevation and vertical land movement data than to variations in population data in the study area. The inclusion of local information on human induced subsidence rates increases the relative sea-level by 60cm in 2100, resulting in coastal flood impacts that are up to 25% higher compared to those generated with the global DIVA values, which mainly account for natural processes. The choice of one elevation model over another can result in differences of approximately 45% of the coastal floodplain extent and up to 50% in flood damages by 2100. Our results emphasize that the scale of assessment and resolution of the input data can have significant implications for the results of coastal flood impact assessments. Understanding and communicating these implications is

  19. Free Hugs: Dinâmicas de troca, dádiva e estranhamento na intervenção urbana

    Directory of Open Access Journals (Sweden)

    Fernando Gil Paiva Martins

    2012-06-01

    Full Text Available Este artigo visa analisar a campanha mundial Free Hugs (Abraços Grátis, que se transformou de ação individual no ano de 2001 na Austrália em mobilização urbana desde 2003 com os flash mobs inventados em Nova York e depois realizados em escala global. A partir disso, explorar as dinâmicas de troca simbólica no ato de oferecer enfaticamente abraços grátis a partir do conceito de dádiva de Marcel Mauss, tendo como condição histórica o atual estágio de desenvolvimento do capitalismo. Na difusão viral de ideias pelo ciberespaço, que permite mostrar como as cidades tornaram-se nós de produção e reprodução de informações, toma-se a campanha do Free Hugs em Cuiabá, nas bordas do Pantanal em Mato Grosso, em sua potência de mobilização social e como ilustração deste processo de trocas nos fluxos da globalização.

  20. A críticada razão utilitária e a fundamentação ontológica das escolhas metodológicas no paradigma da dádiva

    Directory of Open Access Journals (Sweden)

    Alexandre Simão de Freitas

    2002-12-01

    Full Text Available Neste artigo, procura-se demonstrar que a crítica da razão utilitária, processada pelo paradigma da dádiva contribui para uma reflexão radical das escolhas metodológicas no âmbito das ciências sociais. Ao buscar interrogar o vínculo social, o paradigma da dádiva aciona um terceiro nível de análise da ação social: o nível das redes e das relações. No âmbito ontológico, isso permite que as teorias sociológicas transcendam, simultaneamente, as aporias do holismo e do individualismo metodológico, abrindo novas vias de descrição, explicação, interpretação e avaliação do funcionamento das regras do social.

  1. O discurso da violência sistêmico-simbólica e sua replicação nos memes de humor da fanpage Diva Depressão

    OpenAIRE

    Soares, Pricilla Farina

    2013-01-01

    Esta pesquisa tem como objetivo o estudo do discurso da violência sistêmico-simbólica presente nos memes da fanpage do Facebook, Diva Depressão, a fim de analisar a legitimação e a disseminação da violência mascarada pelo humor. O intuito é discutir o que são memes, como eles se projetam em sites de redes sociais e qual a ligação que o discurso da violência sistêmico-simbólica, implicada nos comentários e apropriações das ferramentas do site analisado tem com estes memes. O trabalho tem como ...

  2. Detection of Mycoplasma mycoides subsp. mycoides SC in bronchoalveolar lavage fluids of cows based on a TaqMan real-time PCR discriminating wild type strains from an lppQ− mutant vaccine strain used for DIVA-strategies

    Science.gov (United States)

    Vilei, Edy M.; Frey, Joachim

    2010-01-01

    Contagious bovine pleuropneumonia (CBPP) is the most serious cattle disease in Africa, caused by Mycoplasma mycoides subsp. mycoides small-colony type (SC). CBPP control strategies currently rely on vaccination with a vaccine based on live attenuated strains of the organism. Recently, an lppQ− mutant of the existing vaccine strain T1/44 has been developed (Janis et al., 2008). This T1lppQ− mutant strain is devoid of lipoprotein LppQ, a potential virulence attribute of M. mycoides subsp. mycoides SC. It is designated as a potential live DIVA (Differentiating Infected from Vaccinated Animals) vaccine strain allowing both serological and etiological differentiation. The present paper reports on the validation of a control strategy for CBPP in cattle, whereby a TaqMan real-time PCR based on the lppQ gene has been developed for the direct detection of M. mycoides subsp. mycoides SC in ex vivo bronchoalveolar lavage fluids of cows and for the discrimination of wild type strains from the lppQ− mutant vaccine strain. PMID:20381545

  3. Fazendo guanxi: dádivas, etiquetas e emoções na economia da China pós-Mao Making Guanxi: gifts, etiquettes and emotions in the economy of post-mao China

    Directory of Open Access Journals (Sweden)

    Rosana Pinheiro-Machado

    2011-04-01

    Full Text Available Este artigo trata da importância das redes de relações pessoais no processo de abertura para a economia de mercado da China contemporânea. O sistema de guanxi, que significa conexões interpessoais de ajuda mútua, ao invés de desaparecer ao longo da reforma econômica, fortaleceu-se sobremaneira, constituindo-se como elemento êmico crucial para o desenvolvimento de negócios nas mais diversas esferas econômicas, locais e globais. Esse modo sui generis entrelaça autoridades do Estado e empresários, que são as novas elites do país, e atualiza antigos cerimoniais de dádivas. À luz do método etnográfico, discuto esse tema, apresentando três casos que representam meu processo de formação de guanxi durante trabalho de campo na Província de Guangdong.The article discusses the importance of personal networks in opening up the contemporary Chinese economy to market forces. Instead of disappearing in the process of economic reform, the system of guanxi- a word signifying interpersonal connections based on mutual help- has actually strengthened hugely, becoming a crucial emic element in the development of business among the most diverse economic spheres, both local and global. This sui generis mode brings together authorities and business people- who form the country's new elite- and reproduces traditional gift ceremonies in new ways. Using an ethnographic methodology, zI discuss the topic through the presentation of three cases illustrating my process of making guanxi during fieldwork in Guangdong Province

  4. 关于DIVA模型中语速对语音生成影响的研究%Study of Speaking Rate Effects to Production of Speech Sounds in DIVA Model

    Institute of Scientific and Technical Information of China (English)

    刘燕燕; 张少白

    2011-01-01

    Mainly study the speaking rate effects to the production of speech sounds. Many researchers in the past twenty years has investigated this,and they pointed that changes in speaking rate have differential effects for the movements corresponding to vowels and consonants of speech sounds,but they didn' t point what the exact effects are. Based on the DIVA (Directions Into Velocities of Artculators) model,discussed and investigated these effects by modifying the ODV ( Orosensory Direction Vector) and AW ( Articulator Velocity Vector) equations. The simulation results show that increasing rate causes an increase in the velocities of movements corresponding to consonantal gestures,but it causes less of an increase,or even a decrease,in the velocities of movements corresponding to vowel gestures. The final conclusion is:despite changes in speaking rate have differential effects for the movements correspondng to vowels and consonants, the increase in ratios of maximum velocity to movement distance for the vowel and consonant gestures are almost same to vowels and consonants.%着重研究语速对语音生成的影响.过去有不少人对此进行了研究,指出语速的变化对元音和辅音的运动产生了不同的影响,但是具体是什么样的影响并没有明确指出.基于DIVA模型,通过修改ODV和AVV活动的公式对语速对元音和辅音运动产生的影响进行了研究和探讨.仿真实验表明,语速的增加导致了辅音运动速度的增加,但元音运动速度只增加了少量的值,有时甚至还会减小.最终得出的结论是:尽管语速对元音和辅音的运动速度产生了不同的影响,但最大速度与运动距离比值的增量却是大致相同的.

  5. Exchange networks and free shops in Berlin: gifts and commodities in ‘alternative’ consumption experiences Redes de intercâmbio e lojas gratuitas em Berlim: dádiva e mercadoria em experiências “alternativas” de consumo

    Directory of Open Access Journals (Sweden)

    Irene Sabaté Muriel

    2009-05-01

    Full Text Available This paper is based on an ethnography of ‘alternative’ consumption practices in the inner city of the former East Berlin. Non-monetary exchange networks (Tauschringe and ‘free shops’ (Umsonstläden have been examined. In Umsonstläden, the contemporary ideology of the ‘pure’ gift (Parry 1986; Carrier 1995 is at play: objects are freely given and totally alienated from their owners. In turn, Tauschringe sometimes induce gift-giving practices entailing mutual obligation, as a result of frequent exchanges which bring participants socially closer. The ethnographic material I present challenges the suitability of a conceptual gift/commodity divide to examine these experiences, provided that different modalities of gift-giving are articulated with commoditisation trends. Considering this complexity, I propose a re-examination of the role of the gift in ‘alternative’ consumption practices promoted by social movements in Berlin.O artigo baseia-se numa etnografia de práticas de consumo “alternativas” nos bairros centrais da antiga Berlim-Leste. São aqui analisadas as redes de intercâmbio não monetário (Tauschringe e as “lojas gratuitas” (Umsonstläden. As Umsonstläden são enquadradas pela ideologia contemporânea da dádiva “pura” (Parry 1986; Carrier 1995: os objectos são dados gratuitamente e totalmente alienados dos seus possuidores. Já as Tauschringe, por seu lado, induzem por vezes práticas de oferta que implicam obrigações mútuas, como consequência de trocas frequentes que aproximam socialmente os participantes. O material etnográfico apresentado põe em causa a adequação do binómio dádiva/mercadoria à análise dessas experiências, na medida em que diferentes modalidades de oferta se conjugam com tendências para a mercadorização. Face a essa complexidade, o artigo propõe uma revisão da análise do papel da dádiva em práticas de consumo “alternativas” promovidas pelos movimentos sociais em

  6. Diva Maria, rolevaja zhenshtshina / Ksenia Repson

    Index Scriptorium Estoniae

    Repson, Ksenia

    2002-01-01

    Ajaleht asutas iga-aastase preemia "Sild", mis antakse panuse eest eesti- ja muukeelse elanikkonna integreerimisel. Esimeseks laureaadiks on M. Avdjushko, kes mängis peaosa Vene Draamateatri lavastuses "Äike"

  7. Divas informatīvās telpas Latvijas presē valsts nacionālo svētku diskursā ("Diena", "Neatkarīgā Rīta Avīze", "Čas", "Telegraf" 2006., 2007.g.)

    OpenAIRE

    Pastare, Laila

    2008-01-01

    Bakalaura darbā „Divas informatīvās telpas Latvijas presē valsts nacionālo svētku diskursā. („Diena”, „Neatkarīgā Rīta Avīze”, „Čas”, „Telegraf” 2006.; 2007. g.)” tiek pētīts, kādas atšķirības pastāv starp divām informatīvajām telpām valsts nacionālo svētku diskursā un kā tās izpaužas. Bakalaura darba teorētiskā bāze balstās uz jau esošajiem Ilzes Šulmanes un Sergeja Kruka konceptiem par atšķirīgajām latviešu un krievu informatīvajām telpām, un šo pašu autoru iegūtajiem rezultātiem un sec...

  8. Experimental vaccinations for avian influenza virus including DIVA approaches

    Science.gov (United States)

    Avian influenza (AI) is a viral disease of poultry that remains an economic threat to commercial poultry throughout the world by negatively impacting animal health and trade. Strategies to control avian influenza (AI) virus are developed to prevent, manage or eradicate the virus from the country, re...

  9. A DÁDIVA DA AGRESSÃO

    Directory of Open Access Journals (Sweden)

    MARIA DO SOCORRO LACERDA LIMA

    2009-12-01

    Full Text Available On the context of Tupinambá war, bodies, trophies, women, children, names, words, identities, aggressions, offenses, and a lot more richness material that from the changing elements moving on permanently among enemy groups. But on the contrary, the potlatch held on the American northwest, where the alliance establishes a mutual relation of favors between not enemy groups. On the context of Tupi war, the changing system is based exactly in a hostilerelation among opposite groups. The aim of the present article is to establish a parallel between anthropophagic complexes of Tupinambá Indians and established potlatch on the American’s northwest societies analyzed by Marcel Mauss.RESUMO: No contexto da guerra tupinambá corpos, troféus, mulheres, crianças, nomes, palavras, identidades, agressões, ofensas e muitas outras riquezas materiais e imateriais se constituem em elementos de troca que circulam perpetuamente entre grupos inimigos. Mas, aocontrário do que ocorre no Potlatch realizado no noroeste americano, em que a aliança estabelece uma relação mútua de prestações entre grupos não inimigos, no contexto da guerra Tupi o sistema de trocas baseia-se exatamente na relação hostil entre grupos contrários. Oobjetivo do presente artigo é tecer um paralelo entre o complexo antropofágico dos índios Tupinambá e o Potlatch estabelecido entre as sociedades do Noroeste Americano analisadas por Marcel Mauss.

  10. A DIVA vaccine for cross-protection against Salmonella

    Science.gov (United States)

    Swine are often asymptomatic carriers of Salmonella spp., a leading cause of human bacterial foodborne disease. Vaccination against Salmonella is effective for protection of animal health and enhancement of food safety. However, current vaccines for swine may only offer limited cross-protection agai...

  11. DIVA: an iterative method for building modular integrated models

    Science.gov (United States)

    Hinkel, J.

    2005-08-01

    Integrated modelling of global environmental change impacts faces the challenge that knowledge from the domains of Natural and Social Science must be integrated. This is complicated by often incompatible terminology and the fact that the interactions between subsystems are usually not fully understood at the start of the project. While a modular modelling approach is necessary to address these challenges, it is not sufficient. The remaining question is how the modelled system shall be cut down into modules. While no generic answer can be given to this question, communication tools can be provided to support the process of modularisation and integration. Along those lines of thought a method for building modular integrated models was developed within the EU project DINAS-COAST and applied to construct a first model, which assesses the vulnerability of the world's coasts to climate change and sea-level-rise. The method focuses on the development of a common language and offers domain experts an intuitive interface to code their knowledge in form of modules. However, instead of rigorously defining interfaces between the subsystems at the project's beginning, an iterative model development process is defined and tools to facilitate communication and collaboration are provided. This flexible approach has the advantage that increased understanding about subsystem interactions, gained during the project's lifetime, can immediately be reflected in the model.

  12. Diva Carmen Luisa Letelier Valdés, contralto

    Directory of Open Access Journals (Sweden)

    Valene Georges

    2012-06-01

    Full Text Available Carmen Luisa Letelier Valdés y Valene Georges son fundadoras del célebre Ensemble Bartok Chile. En este trabajo Valene Georges presenta un recuerdo tanto de los titánicos logros que ambas han alcanzado con el Ensemble Bartok Chile en el país como en el extranjero, como de la estrecha amistad que las ha unido durante más de treinta años.Both Carmen Luisa Letelier-Valdés and Valene Georgesfounded the renowned Ensemble Bartok Chile. In this article Valene Georges presents a retrospective view ofthe titanic achievements that both have shared with this ensemble in Chile and abroad. Besides, the author presents a personal view ofher close friendship with Carmen Luisa during more than thirty years.

  13. Spatial variations of sea-level rise and impacts: an application of DIVA

    OpenAIRE

    Brown, S; Nicholls, R.J.; Lowe, J A; Hinkel, J.

    2013-01-01

    Due to complexities of creating sea-level rise scenarios, impacts of climate-induced sea-level rise are often produced from a limited number of models assuming a global uniform rise in sea level. A greater number of models, including those with a pattern reflecting regional variations would help to assure reliability and a range of projections, indicating where models agree and disagree. This paper determines how nine new patterned-scaled sea-level rise scenarios (plus the uniform and pattern...

  14. Rosa de Vries: A Dutch Diva and Nineteenth-Century Trans-Atlantic Operatic Culture

    NARCIS (Netherlands)

    Metzelaar, H.

    2014-01-01

    In an arduous, twenty-year international career, Dutch opera soprano Rosa de Vries née van Os (1824-89) performed both French and Italian opera repertory with leading companies on both sides of the Atlantic. Experiencing triumphs and disappointments while managing both career and family, de Vries

  15. Rosa de Vries: A Dutch Diva and Nineteenth-Century Trans-Atlantic Operatic Culture

    NARCIS (Netherlands)

    Metzelaar, H.

    2014-01-01

    In an arduous, twenty-year international career, Dutch opera soprano Rosa de Vries née van Os (1824-89) performed both French and Italian opera repertory with leading companies on both sides of the Atlantic. Experiencing triumphs and disappointments while managing both career and family, de Vries re

  16. The Diva and the Beast: Susan Strong and the Wagnerism of Aleister Crowley

    OpenAIRE

    Allis, MJ

    2014-01-01

    Although the occultist Aleister Crowley (1875-1947) remains a fascinating and controversial figure, his connections with Richard Wagner have not been fully explored. In identifying several of Crowley’s literary works from c.1900 onwards (poetry, prose, drama) that make overt reference to Wagner’s compositions, this article suggests one possible catalyst for this burst of activity – the American soprano Susan Strong (1870-1946), to whom Crowley was briefly ‘engaged’ (according to his autohagio...

  17. Historical Biogeography of a Clade of Liolaemus (Iguania: Liolaemidae based on ancestral areas and dispersal-vicariance analysis (DIVA

    Directory of Open Access Journals (Sweden)

    Juan Manuel Díaz Gómez

    2006-01-01

    Full Text Available Although many phylogenetic studies have been made on the genus Liolaemus, they are inappropriate for a biogeographic study because of incomplete taxon sampling. Here we develop a biogeographic analysis using a new phylogenetic study of the chiliensis group, which differs from the other studies in having his members fully represented. The biogeographic analysis presented here consists in reconstructing the ancestral area of this clade, under three different methods of analysis: Fitch Optimisation, Dispersal Vicariance Analysis and Weighted Ancestral Area Analysis. The analyses were made on phylogenies from parsimony under equally weighted characters, as well as under implied weighting. The equally weighted tree recovers most of the groupings proposed in previous studies. The strict consensus of the implied weighting trees recovers fifteen groups, but there is no agreement of the relationships between those groups. The ancestral area analyses agree in an Andean-Patagonian origin for the chiliensis clade, and are congruent with previous hypotheses and paleontological data.Ainda que tenham sido feito muitos estudos filogenéticos no gênero Liolaemus, os mesmos não são apropriados para uma análise biogeográfica, porque não possuem uma amostragem taxonômica adequada. Desenvolve-se neste trabalho, uma análise biogeográfica do grupo de L. chiliensis, que se diferencia das contribuições prévias por representar a todos os membros do grupo. A análise biogeográfica consiste em reconstruir a área ancestral deste clado, utilizando três métodos diferentes: Otimização de Fitch, Análise de Dispersão-Vicariância e Áreas Ancestrais Pesadas. As análises foram realizadas em filogenias feitas com o critério de parsimônia sob pesos iguais e pesos implicados. A árvore obtida sob pesos iguais, recupera a maioria dos grupos encontrados em análises prévias. O consenso estrito das árvores de pesos implicados, recupera quinze grupos, mas não pode-se estabelecer as relações entre eles. A análise de áreas ancestrais permite postular a região Andino-Patagônica como provável área ancestral, resultado este, congruente com hipóteses prévias e com informação paleontológica.

  18. An Educational Exploration of Homophobia and Sexism in Rap and Hip Hop: Homo-Thugs and Divas in da House

    Science.gov (United States)

    Chiu, Nicholas

    2005-01-01

    In this paper, the author explores the connections between hip hop and rap, sexism and homophobia, and children and teens. He describes the implications or potential consequences of sexism and homophobia within the music and media culture of hip hop and rap (with the focus on how it affects young viewers and fans in terms of gender [identity]…

  19. Nem holismo nem individualismo metodológicos: Marcel Mauss e o paradigma da dádiva

    Directory of Open Access Journals (Sweden)

    Caillé Alain

    1998-01-01

    Full Text Available O artigo se situa no contexto da crítica ao utilitarismo que vem sendo conduzida, desde o início dos anos 80, por um grupo de intelectuais reunidos em torno do MAUSS - Movimento Antiutilitarista nas Ciências Sociais, cujas idéias são divulgadas pela La Revue du MAUSS, publicada pela Editora La Découverte, em Paris. Alain Caillé procura resgatar a importância de Marcel Mauss no interior da escola sociológica francesa, ressaltando a contribuição deste autor clássico na sistematização inicial do paradigma do dom, que subsiste na modernidade, como o demonstram, em várias oportunidades, os colaboradores deste movimento antiutilitarista. Para Caillé, o paradigma do dom constitui o fundamento necessário de criação de uma teoria paradoxal e pluridimensional da ação, capaz de resolver a dicotomia clássica entre holismo e individualismo. No entanto, ressalta o diretor da Revue du MAUSS, na constituição do novo paradigma, as imbricações entre o dom e o simbolismo ainda permanecem imprecisas e algo misteriosas.

  20. 'Well I'm still the Diva!' Enabling people with dementia to express their identity through graffiti arts: Innovative practice.

    Science.gov (United States)

    Hicks, Ben; Carroll, Denise; Shanker, Shanti; El-Zeind, Angela

    2017-01-01

    This article reports on a pilot study that investigated the use of graffiti arts as a medium for promoting self-expression in people with dementia. Two people with dementia attended a series of workshops with a graffiti artist where they explored their feelings of changing identity following their dementia diagnoses. As part of the workshops, they were encouraged to develop a personal 'tag' or signature to portray their sense of identity and a piece of street art to express 'their message'. These completed artworks were displayed in a public space in Bournemouth, UK.

  1. Aletamine maastikukujunduse- ja kultuuritegurina Põhja- Euroopas ja Eestis / Ülle Tarkiainen

    Index Scriptorium Estoniae

    Tarkiainen, Ülle, 1957-

    2014-01-01

    Aletamise vormidest. Alesavolastest ja ale põletamisega seotud müütidest. Alepõletamise algusest Eestis. Aletamise kajastumisest 17. sajandi allikmaterjalides. Metsapõldudest viljatootmise ja uusasustuse ressursina. Aletamise marginaliseerumisest.

  2. Aletamine maastikukujunduse- ja kultuuritegurina Põhja- Euroopas ja Eestis / Ülle Tarkiainen

    Index Scriptorium Estoniae

    Tarkiainen, Ülle, 1957-

    2014-01-01

    Aletamise vormidest. Alesavolastest ja ale põletamisega seotud müütidest. Alepõletamise algusest Eestis. Aletamise kajastumisest 17. sajandi allikmaterjalides. Metsapõldudest viljatootmise ja uusasustuse ressursina. Aletamise marginaliseerumisest.

  3. VP2-serotyped live-attenuated bluetongue virus without NS3/NS3a expression provided serotype-specific protection and enables DIVA.

    NARCIS (Netherlands)

    Feenstra, F.; Maris-Veldhuis, M.A.; Daus, F.J.; Tacken, M.G.J.; Moormann, R.J.M.; Gennip, van H.G.P.; Rijn, van P.A.

    2014-01-01

    Bluetongue virus (BTV) causes Bluetongue in ruminants and is transmitted by Culicoides biting midges. Vaccination is the most effective measure to control vector borne diseases; however, there are 26 known BTV serotypes showing little cross protection. The BTV serotype is mainly determined by genome

  4. Para Além do Estado e do Mercado: A Dádiva no Fenômeno dos Sem-Abrigo

    Directory of Open Access Journals (Sweden)

    João Aldeia

    2014-03-01

    Full Text Available Las reflexiones sobre el fenómeno de los sin techo tienden a reproducir las ausencias que el paradigma del don identifica en el pensamiento sobre las sociedades modernas occidentales. Recurrentemente, se considera al Estado y al mercado como las únicas esferas de regulación social y se los interpreta como los espacios de donde derivan las causas estructurales y las posibles soluciones para el fenómeno. Ello no permite una comprensión completa del tema y tampoco una reflexión consistente sobre los modos de minimizar las injusticias de la vida en la calle. Pensar sobre el fenómeno por medio del paradigma del don es relevante porque permite identificar la habitual invisibilización de las relaciones interpersonales no oficiales que caracterizan la vida en la calle, posibilitando, a su vez, la revalorización de estas interacciones en el debate sobre el fenómeno de los sin techo.

  5. An eighteenth-century jealous woman and a twentieth-century hysterical diva: the case of Mozart's Idomeneo (1781) and Strauss' Elektra (1909)

    OpenAIRE

    Bakogianni, Anastasia

    2007-01-01

    This paper examines two examples of Electra's reception in the artistic medium of opera. The methodology used in this analysis involves examining the historical, socio-political and cultural context of each reception. That is then followed by a detailed comparative analysis between the original source and the adaptation. This method focuses on how the context of each reception influenced the changes made to the original.

  6. The effects of adaptation and mitigation on coastal flood impacts during the 21st century. An application of the DIVA and IMAGE models

    NARCIS (Netherlands)

    Hinkel, J.; Vuuren, D.P. van; Nicholls, R.J.; Klein, R.J.T.

    2012-01-01

    This paper studies the effects of mitigation and adaptation on coastal flood impacts. We focus on a scenario that stabilizes concentrations at 450 ppm-CO 2-eq leading to 42 cm of global mean sea-level rise in 1995-2100 (GMSLR) and an unmitigated one leading to 63 cm of GMSLR. We also consider sensit

  7. A new Cumacea genus (Crustacea: Peracarida: Diastylidae) from the deep-sea expedition Diva-1 with RV ‘METEOR’ to the Angola Basin

    NARCIS (Netherlands)

    Mühlenhardt-Siegel, Ute

    2003-01-01

    A new deep-sea genus and species Divacuma tuerkayi n. gen., n. sp. of the family Diastylidae from the Angola Basin is described. The similarity between the new genus and Leptostylis/Leptostybides is mentioned, the most striking character of the new genus is the male having only one pair of pleopods.

  8. Raltegravir concentrations in the genital tract of HIV-1-infected women treated with a raltegravir-containing regimen (DIVA 01 study).

    Science.gov (United States)

    Clavel, Cyril; Peytavin, Gilles; Tubiana, Roland; Soulié, Cathia; Crenn-Hebert, Catherine; Heard, Isabelle; Bissuel, François; Ichou, Houria; Ferreira, Claudia; Katlama, Christine; Marcelin, Anne-Geneviève; Mandelbrot, Laurent

    2011-06-01

    We studied the penetration of raltegravir and HIV shedding in the genital tract among 14 HIV-1-infected women receiving a raltegravir-containing regimen who had <40 copies/ml blood plasma (BP) HIV RNA. None of the cervicovaginal fluid (CVF) samples showed detectable HIV RNA. Median raltegravir concentrations were 235 ng/ml in BP and 93 ng/ml in CVF, with a CVF/BP ratio of approximately 2.3. This good penetration of raltegravir may contribute to the control of viral replication in the female genital tract.

  9. Harvesting an experiment: observations of bushy landrace winter rye in a small scale experimental slah-and-burn field in Estonia / Kersti Kihno, Liisi Jääts, Mati Laane

    Index Scriptorium Estoniae

    Kihno, Kersti, 1963-

    2015-01-01

    13. sajandi alguseks kujunes talirukis Eesti ala tähtsaimaks toiduteraviljaks ning oli 13. - 18. sajandini ka peamine ekspordiartikkel. Alepõllul kasvatatud puhmasrukis andis suuri saake, kuid oli nõudlik maa viljakuse suhtes. Eksperiment toimus Tartumaal. Eksperimendi tulemused kinnitasid ajalooallikate kirjeldusi - soodsates tingimustes on puhmasrukis väga saagikas. Kuigi väiksemõõtmelise eksperimendi tulemusi ei saa käsitleda kui suure alepõllu täpset mudelit, annab see siiski olulist materjali talirukki maasordi kohta, mis on siiani saanud teenimatult vähe tähelepanu

  10. Harvesting an experiment: observations of bushy landrace winter rye in a small scale experimental slah-and-burn field in Estonia / Kersti Kihno, Liisi Jääts, Mati Laane

    Index Scriptorium Estoniae

    Kihno, Kersti, 1963-

    2015-01-01

    13. sajandi alguseks kujunes talirukis Eesti ala tähtsaimaks toiduteraviljaks ning oli 13. - 18. sajandini ka peamine ekspordiartikkel. Alepõllul kasvatatud puhmasrukis andis suuri saake, kuid oli nõudlik maa viljakuse suhtes. Eksperiment toimus Tartumaal. Eksperimendi tulemused kinnitasid ajalooallikate kirjeldusi - soodsates tingimustes on puhmasrukis väga saagikas. Kuigi väiksemõõtmelise eksperimendi tulemusi ei saa käsitleda kui suure alepõllu täpset mudelit, annab see siiski olulist materjali talirukki maasordi kohta, mis on siiani saanud teenimatult vähe tähelepanu

  11. Replacement of saturated with unsaturated fats had no impact on vascular function but beneficial effects on lipid biomarkers, E-selectin, and blood pressure: results from the randomized, controlled Dietary Intervention and VAScular function (DIVAS) study.

    Science.gov (United States)

    Vafeiadou, Katerina; Weech, Michelle; Altowaijri, Hana; Todd, Susan; Yaqoob, Parveen; Jackson, Kim G; Lovegrove, Julie A

    2015-07-01

    Public health strategies to lower cardiovascular disease (CVD) risk involve reducing dietary saturated fatty acid (SFA) intake to ≤10% of total energy (%TE). However, the optimal type of replacement fat is unclear. We investigated the substitution of 9.5-9.6%TE dietary SFAs with either monounsaturated fatty acids (MUFAs) or n-6 (ω-6) polyunsaturated fatty acids (PUFAs) on vascular function and other CVD risk factors. In a randomized, controlled, single-blind, parallel-group dietary intervention, 195 men and women aged 21-60 y from the United Kingdom with moderate CVD risk (≥50% above the population mean) followed one of three 16-wk isoenergetic diets (%TE target compositions, total fat:SFA:MUFA:n-6 PUFA) that were rich in SFAs (36:17:11:4, n = 65), MUFAs (36:9:19:4, n = 64), or n-6 PUFAs (36:9:13:10, n = 66). The primary outcome measure was flow-mediated dilatation; secondary outcome measures included fasting serum lipids, microvascular reactivity, arterial stiffness, ambulatory blood pressure, and markers of insulin resistance, inflammation, and endothelial activation. Replacing SFAs with MUFAs or n-6 PUFAs did not affect the percentage of flow-mediated dilatation (primary endpoint) or other measures of vascular reactivity. Of the secondary outcome measures, substitution of SFAs with MUFAs attenuated the increase in night systolic blood pressure (-4.9 mm Hg, P = 0.019) and reduced E-selectin (-7.8%, P = 0.012). Replacement with MUFAs or n-6 PUFAs lowered fasting serum total cholesterol (-8.4% and -9.2%, respectively), low-density lipoprotein cholesterol (-11.3% and -13.6%), and total cholesterol to high-density lipoprotein cholesterol ratio (-5.6% and -8.5%) (P ≤ 0.001). These changes in low-density lipoprotein cholesterol equate to an estimated 17-20% reduction in CVD mortality. Substitution of 9.5-9.6%TE dietary SFAs with either MUFAs or n-6 PUFAs did not significantly affect the percentage of flow-mediated dilatation or other measures of vascular function. However, the beneficial effects on serum lipid biomarkers, blood pressure, and E-selectin offer a potential public health strategy for CVD risk reduction. This trial was registered at www.clinicaltrials.gov as NCT01478958. © 2015 American Society for Nutrition.

  12. Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs.

    Science.gov (United States)

    Yi, Li; Cheng, Shipeng; Xu, Hongli; Wang, Jianke; Cheng, Yuening; Yang, Shen; Luo, Bin

    2012-01-01

    A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to detect only CDV wild-type strains. A 335bp fragment was amplified from the genomic RNA of the vaccine and wild-type strains. A 555bp fragment was amplified specifically from the genomic RNA of the wild-type strains. No amplification was achieved for the uninfected cells, cells infected with canine parvovirus, canine coronavirus, or canine adenovirus. The combined RT-PCR method detected effectively and differentiated the CDV wild-type and vaccine strains by two separate RT-PCRs. The method can be used for clinical detection and epidemiological surveillance. The phylogenetic analysis of the hemagglutinin gene of the local wild-type CDV strains revealed that the seven local isolates all belonged to the Asia-1 lineage, and were clustered closely with one another at the same location. These results suggested that the CDV genotype Asia-1 is circulating currently in domestic dogs in China.

  13. A Neuroimaging Study of Premotor Lateralization and Cerebellar Involvement in the Production of Phonemes and Syllables

    Science.gov (United States)

    Ghosh, Satrajit S.; Tourville, Jason A.; Guenther, Frank H.

    2008-01-01

    Purpose: This study investigated the network of brain regions involved in overt production of vowels, monosyllables, and bisyllables to test hypotheses derived from the Directions Into Velocities of Articulators (DIVA) model of speech production (Guenther, Ghosh, & Tourville, 2006). The DIVA model predicts left lateralized activity in inferior…

  14. Method to Select Technical Terms for Glossaries in Support of Joint Task Force Operations

    Science.gov (United States)

    2012-01-01

    goals. While the present method underpins a capability that serves only one specific group, the foundation of the method explained here justifies...material that supports the bilingual work. Thus, physicians want to see medical terms, attorneys want to see legal terms, and Soldiers want to see...prepared for one document in particular, the first text in Stanford Law School’s Afghan Legal Education Project (ALEP), An Introduction to the Law of

  15. Genetic relationship among Musa genotypes revealed by ...

    African Journals Online (AJOL)

    enoh

    2012-03-29

    Mar 29, 2012 ... 2004). Researchers believe their center of diversity to be either Malaysia or ... and Bario, two locations belonging to two different divisions in ..... Literature on the Distribution of Wild Musa Species Using MGIS and. DIVA-GIS.

  16. Uuenenud menuansambel Tuberkuloited tuuritab

    Index Scriptorium Estoniae

    2007-01-01

    Rockansamblist Tuberkuloited, kontsertidest 13. sept. Tartus klubis Illusion, 14. sept. Kuressaare ööklubis Diva, 15. sept. Pärnu klubis Strand, 20. sept. Tallinnas Von Krahlis ja 21. sept. Kärdlas. Heliplaadist "Mis sa teed?"

  17. Effects of alkaline earth metal ion complexation on amino acid zwitterion stability: Results from infrared action spectroscopy

    NARCIS (Netherlands)

    Bush, M. F.; Oomens, J.; Saykally, R. J.; Williams, E. R.

    2008-01-01

    The structures of isolated alkaline earth metal cationized amino acids are investigated using infrared multiple photon dissociation (IRMPD) spectroscopy and theory. These results indicate that arginine, glutamine, proline, serine, and valine all adopt zwitterionic structures when complexed with diva

  18. Toward a sustainable waste management system: a comprehensive assessment of thermal and electric energy recovery from waste incineration

    Energy Technology Data Exchange (ETDEWEB)

    Salvia, Monica; Cosmi, Carmelina; Cuomo, Vincenzo; Macchiato, Maria [Istituto Nazionale per la Fisica della Materia, Napoli (Italy); Mangiamele, Lucia [Univ. della Basilicata, Potenza (Italy). Dipt. Ingegneria e Fisica dell Ambiente; Pietrapertosa, Filomena [Univ. di Napoli Federico II, (Italy). Dipt. di Scienze Fisiche

    2002-12-01

    Energy-environmental planning must join normative, environmental and socio-economic features to obtain effective strategies aimed to a sustainable development. Therefore a comprehensive methodology for the analysis and the optimisation of the anthropogenic activities system configuration, can usefully support decision-makers in the definition of harmonised sector plans, joining waste management issues with resource use problems and exploiting energy and materials feedback among supply and demand sectors. In this paper we present an innovative application of the Advanced Local Energy Environmental Planning methodology (ALEP), aimed to the definition of optimal waste management strategies which comply with comprehensive as well as sectorial issues.

  19. Entropy-Based Model for Interpreting Life Systems in Traditional Chinese Medicine

    Directory of Open Access Journals (Sweden)

    Guo-lian Kang

    2008-01-01

    Full Text Available Traditional Chinese medicine (TCM treats qi as the core of the human life systems. Starting with a hypothetical correlation between TCM qi and the entropy theory, we address in this article a holistic model for evaluating and unveiling the rule of TCM life systems. Several new concepts such as acquired life entropy (ALE, acquired life entropy flow (ALEF and acquired life entropy production (ALEP are propounded to interpret TCM life systems. Using the entropy theory, mathematical models are established for ALE, ALEF and ALEP, which reflect the evolution of life systems. Some criteria are given on physiological activities and pathological changes of the body in different stages of life. Moreover, a real data-based simulation shows life entropies of the human body with different ages, Cold and Hot constitutions and in different seasons in North China are coincided with the manifestations of qi as well as the life evolution in TCM descriptions. Especially, based on the comparative and quantitative analysis, the entropy-based model can nicely describe the evolution of life entropies in Cold and Hot individuals thereby fitting the Yin–Yang theory in TCM. Thus, this work establishes a novel approach to interpret the fundamental principles in TCM, and provides an alternative understanding for the complex life systems.

  20. Mechanical and Electrical Properties of Aluminum/Epoxy Nanocomposites

    Science.gov (United States)

    Dong, Lina; Zhou, Wenying; Sui, Xuezhen; Wang, Zijun; Cai, Huiwu; Wu, Peng; Zhang, Yating; Zhou, Anning

    2016-11-01

    Surface-modified self-passivated aluminum (Al) nanoparticles were used for reinforcing epoxy (EP) resin, and the curing behavior, mechanical and electrical properties of the Al/EP nanocomposites were investigated. The incorporation of Al nanoparticles into EP significantly decreases the cure reaction enthalpy of the nancomposites, and the apparent activation energy of Al/EP systems is 64.96 kJ/mol. The coefficient of thermal expansion of the nanocomposites decreases with increasing the Al loading due to the strong interaction between the Al and the EP matrix. The storage modulus of the nanocomposites increases continuously with Al content, whereas, the glass transition temperature declines slightly. With increasing the Al content, the tensile modulus, flexural modulus and compressive modulus of the nanocomposites increase continuously compared with the neat one. The mechanical properties are improved by Al nanoparticles at low Al contents. The best overall dielectric and electrical performance are achieved about at 1 wt.% of Al concentration. The enhanced dielectric breakdown strength is mainly related to the insulating alumina shell on the surface of core Al and the strong interfacial interactions.

  1. Evaluation of CSFV Antibody ELISAs for the differentiation of infected from vacci-nated animals

    DEFF Research Database (Denmark)

    Schroeder, Sabine; Blome, Sandra; Koenen, Frank

    , for their diagnostic sensitivity as well as for their potential in differentiating between infected and marker vaccinated animals. Two newly available ELISAs were included into the tests, the Priocheck® CSFV Erns ELISA, a special DIVA test, and the LDL Pigtype® CSFV Antibody ELISA. An inter-laboratory comparison test......Classical swine fever (CSF) is one of the most important epizootic diseases of pigs. With few exceptions the Member States of the European Union (EU) are currently considered to be free of CSF in domestic pigs. However, the disease is still endemic in the wild boar populations of several European...... of vaccinated from infected animals (DIVA) is not possible. Newly developed modified live marker vaccines allow a DIVA strategy based on the use of enzyme linked immunosorbent assay (ELISA) tests. The aim of this study was to evaluate CSF virus (CSFV) Antibody ELISAs, com-mercially available in Europe...

  2. Dar ou furtar? Presentes, tributos e as ambiguidades da diplomacia luso-africana em Angola à luz dos "sistemas económicos" de Karl Polanyi (séculos 17 a 19)

    OpenAIRE

    De Lima, Dora

    2013-01-01

    O processo das trocas de presentes entre os sucessivos administradores portugueses e os diferentes chefes de etnias em Angola revela, quando analisado na longa duração, algumas das lógicas expostas por Polanyi nos seus diferentes sistemas económicos. Permite superar a dicotomia herdada de Mauss entre as economias de "dádivas" e as de mercado, e julgamos que o próprio termo de "dádiva" perdeu o seu valor heurístico de tal forma que se revela irrelevante para construir uma história das "trocas ...

  3. Systematic study on dynamic atomic layer epitaxy of InN on/in +c-GaN matrix and fabrication of fine-structure InN/GaN quantum wells: Role of high growth temperature

    Science.gov (United States)

    Yoshikawa, Akihiko; Kusakabe, Kazuhide; Hashimoto, Naoki; Hwang, Eun-Sook; Imai, Daichi; Itoi, Takaomi

    2016-12-01

    The growth kinetics and properties of nominally 1-ML (monolayer)-thick InN wells on/in +c-GaN matrix fabricated using dynamic atomic layer epitaxy (D-ALEp) by plasma-assisted molecular beam epitaxy were systematically studied, with particular attention given to the effects of growth temperature. Attention was also given to how and where the ˜1-ML-thick InN layers were frozen or embedded on/in the +c-GaN matrix. The D-ALEp of InN on GaN was a two-stage process; in the 1st stage, an "In+N" bilayer/monolayer was formed on the GaN surface, while in the 2nd, this was capped by a GaN barrier layer. Each process was monitored in-situ using spectroscopic ellipsometry. The target growth temperature was above 620 °C and much higher than the upper critical epitaxy temperature of InN (˜500 °C). The "In+N" bilayer/monolayer tended to be an incommensurate phase, and the growth of InN layers was possible only when they were capped with a GaN layer. The InN layers could be coherently inserted into the GaN matrix under self-organizing and self-limiting epitaxy modes. The growth temperature was the most dominant growth parameter on both the growth process and the structure of the InN layers. Reflecting the inherent growth behavior of D-ALEp grown InN on/in +c-GaN at high growth temperature, the embedded InN layers in the GaN matrix were basically not full-ML in coverage, and the thickness of sheet-island-like InN layers was essentially either 1-ML or 2-ML. It was found that these InN layers tended to be frozen at the step edges on the GaN and around screw-type threading dislocations. The InN wells formed type-I band line-up heterostructures with GaN barriers, with exciton localization energies of about 300 and 500 meV at 15 K for the 1-ML and 2-ML InN wells, respectively.

  4. Sluglett Peter with Weber Stefan (éd., Syria and Bilad al-Sham under Ottoman Rule. Essays in honour of Abdul-Karim Rafeq, Leyde, Brill, 2010, 633 p.

    Directory of Open Access Journals (Sweden)

    Catherine Mayeur-Jaouen

    2013-10-01

    Full Text Available Un an avant le printemps arabe de 2011 qui coïncida avec son quatre-vingtième annniversaire, le grand historien syrien Abdulkarim Rafeq s’était vu offrir en 2010 ce beau volume de Mélanges, fruit d’un colloque tenu en 2004 à Damas et à Beyrouth. La cohérence de l’œuvre de Rafeq (l’histoire du Bilād al-Shām ottoman à travers les archives dont il est un connaisseur hors pair comme le caractère bilingue de sa formation (Collège américain à Alep, Université syrienne à Damas, un doctorat à la SOA...

  5. Les transformations de l'image de Saladin dans les sources occidentales

    OpenAIRE

    Jean Richard

    2009-01-01

    Le personnage de Salāh al-din Yūsuf, officier kurde au service de Nūr al-dïn devenu maître de l'Égypte puis de Damas et enfin d'Alep, et fondateur de l'empire ayyoubide, a été fort controversé de son vivant avant de devenir pour la postérité « le plus pur des héros de l'Islam ». Pour ses adversaires, les Francs d'Orient, il a de bonne heure suscité leur curiosité, et ceux-ci ont recueilli des informations qu'ils ont utilisées, dès avant 1187, dans un Carmen de Saladino où l'aventurier qu'il é...

  6. Some apparently disjoint aims and requirements for grammar development environments the case of natural language generation

    CERN Document Server

    Bateman, J A

    1997-01-01

    Grammar development environments (GDE's) for analysis and for generation have not yet come together. Despite the fact that analysis-oriented GDE's (such as ALEP) may include some possibility of sentence generation, the development techniques and kinds of resources suggested are apparently not those required for practical, large-scale natural language generation work. Indeed, there is no use of `standard' (i.e., analysis-oriented) GDE's in current projects/applications targetting the generation of fluent, coherent texts. This unsatisfactory situation requires some analysis and explanation, which this paper attempts using as an example an extensive GDE for generation. The support provided for distributed large-scale grammar development, multilinguality, and resource maintenance are discussed and contrasted with analysis-oriented approaches.

  7. Final state photon production at LEP

    CERN Document Server

    Ridder, A G D

    1999-01-01

    We present a detailed study of photon production in hadronic events in electron-positron annihilation at LEP energies. We show that estimates of the inclusive photon spectrum using the quark-to-photon fragmentation function determined using the ALEPH `photon' +~1 jet data agree well with the observations of the OPAL collaboration. This agreement shows that the photon fragmentation function determined in this way can be used for inclusive observables. We also compare next-to-leading order and beyond leading logarithm predictions obtained using the numerically resummed solutions of the fragmentation function evolution equation of Bourhis, Fontannaz and Guillet and Glück, Reya and Vogt with the data. Moreover, in order to check the general behaviour of the fragmentation function, we consider an analytic series expansion in the strong coupling. We find that the parameterizations are inaccurate at large $x$ values. While the OPAL data is in broad agreement with estimates based on any of these approaches, the ALEP...

  8. Compressive behavior and energy absorption of metal porous polymer composite with interpenetrating network structure

    Institute of Scientific and Technical Information of China (English)

    LIU Yu; GONG Xiao-lu

    2006-01-01

    Interpenetrating phase composites (IPCs) are a new class of composite materials with improved combinations of mechanical and physical properties. This study was performed on a new type of IPC called metal porous polymer composite (MPPC) with an interpenetrating network structure. Aluminum-polypropylene (Al-PE) and Aluminum-epoxy resin (Al-Ep) composites were produced by infiltrating the polymer in the aluminum foam. The composite microstructures were characterized using SEM observation. The compressive behavior and energy absorption characteristics of MPPC were investigated and compared with the aluminum foams. The compressive modulus of composite was compared with the VOIGT-REUSS bounds and HASHIN-SHTRIKMAN (H-S) bounds models. The experimental modulus of compressive tests falls well within the theoretical models.

  9. The Secret Talents of Fundraisers

    Science.gov (United States)

    Pulley, John

    2010-01-01

    A significant but unknown number of performing artists have redirected their creativity and passion into development. They are ballet and contemporary dancers, jazz and orchestral musicians, actors and comedians, opera divas and gospel belters. None of them set out to become fundraisers. Yet here they are, and they partly credit their success in…

  10. The efficacy of CP7_E2alf: an animal study involving piglets from C–strain vaccinated sows

    DEFF Research Database (Denmark)

    Rangelova, Desislava Yordanova; Nielsen, Jens; Strandbygaard, Bertel;

    Outbreaks of Classical Swine Fever (CSF) in the European Union have caused enormous economical losses. To facilitate the possibility of free trade with pigs and their products, a chimeric live DIVA vaccine CP7_E2alf was developed. Most likely, passive immunity against CSF virus in populations...

  11. Feedforward and Feedback Control in Apraxia of Speech: Effects of Noise Masking on Vowel Production

    Science.gov (United States)

    Maas, Edwin; Mailend, Marja-Liisa; Guenther, Frank H.

    2015-01-01

    Purpose: This study was designed to test two hypotheses about apraxia of speech (AOS) derived from the Directions Into Velocities of Articulators (DIVA) model (Guenther et al., 2006): the feedforward system deficit hypothesis and the feedback system deficit hypothesis. Method: The authors used noise masking to minimize auditory feedback during…

  12. Evaluation of classical swine fever virus antibody detection assays with an emphasis on the differentiation of infected from vaccinated animals

    DEFF Research Database (Denmark)

    Schroeder, S.; von Rosen, Tanya; Blome, S.

    2012-01-01

    vaccinated animals (DIVA). The Chekit* CSF-Sero and the HerdChek* CSFV Ab, both of which detect antibodies against the E2 protein of classical swine fever virus (CSFV), had the highest sensitivity. Both tests were practicable and showed good reproducibility. Comparable sensitivity was shown by the Chekit...

  13. Plaadid / Aleks Lange

    Index Scriptorium Estoniae

    Lange, Aleks

    2003-01-01

    Uutest plaatidest Ricky Martin "Almas del Silencio", Paul McCartney "Back In Th e World", The Ultimate "Diva Collection", Girls Aloud "Sound Of The Underground", Blacky "Kui me päikeseloojangut ootama peaks", Christoph Poppen, Münchner Kammerorchester, The Hilliard Ensemble, Cerys Matthews "Cockahoop", Radiohead "Hail to the Thief"

  14. Creating New Meanings in Leading Learning. Proceedings [of the] National Conference on Alternative and External Degree Programs for Adults (22nd, Pittsburgh, PA, October 9-12, 2002).

    Science.gov (United States)

    American Council on Education, Washington, DC.

    These proceedings are comprised of 25 presentations divided into 5 "tracks." Section I, Diversity and Social Justice, has "Free to Learn with Body and Soul Jazz Divas, Beat Poets, and Street Preachers" (Ferrante, Belcastro); "Clashing Cultures in Our Classrooms" (Gabrich, Rothenberger); "Encouraging Adults in…

  15. Promoting Resiliency in Adolescent Girls through Adventure Programming

    Science.gov (United States)

    Whittington, Anja; Aspelmeier, Jeffery E.; Budbill, Nadine W.

    2016-01-01

    This study examined whether participation in an adventure program increased the resiliency of adolescent girls. Eighty-seven girls who participated in Dirt Divas, a non-profit, adventure program, completed the Resiliency Scale for Children and Adolescents® before and after their experience. Means-comparison tests for within-subjects designs were…

  16. The Secret Talents of Fundraisers

    Science.gov (United States)

    Pulley, John

    2010-01-01

    A significant but unknown number of performing artists have redirected their creativity and passion into development. They are ballet and contemporary dancers, jazz and orchestral musicians, actors and comedians, opera divas and gospel belters. None of them set out to become fundraisers. Yet here they are, and they partly credit their success in…

  17. Plaadid / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2003-01-01

    Uutest plaatidest "Nu Divas 2", New Cool Collective "Bring It On", Kait Tamra "Kõigel on kõigega seos", "Gangs Of New York", george Strait "For The Last Time - Live From The Astrodome", Will Smith "greatest Hits", Sulo "Kuumad tunded", The Donnas "Spend The Night"

  18. Feedforward and Feedback Control in Apraxia of Speech: Effects of Noise Masking on Vowel Production

    Science.gov (United States)

    Maas, Edwin; Mailend, Marja-Liisa; Guenther, Frank H.

    2015-01-01

    Purpose: This study was designed to test two hypotheses about apraxia of speech (AOS) derived from the Directions Into Velocities of Articulators (DIVA) model (Guenther et al., 2006): the feedforward system deficit hypothesis and the feedback system deficit hypothesis. Method: The authors used noise masking to minimize auditory feedback during…

  19. Plaadid / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2003-01-01

    Uutest plaatidest "Nu Divas 2", New Cool Collective "Bring It On", Kait Tamra "Kõigel on kõigega seos", "Gangs Of New York", george Strait "For The Last Time - Live From The Astrodome", Will Smith "greatest Hits", Sulo "Kuumad tunded", The Donnas "Spend The Night"

  20. Outreach Programmes to Attract Girls into Computing: How the Best Laid Plans Can Sometimes Fail

    Science.gov (United States)

    Lang, Catherine; Fisher, Julie; Craig, Annemieke; Forgasz, Helen

    2015-01-01

    This article presents a reflective analysis of an outreach programme called the Digital Divas Club. This curriculum-based programme was delivered in Australian schools with the aim of stimulating junior and middle school girls' interest in computing courses and careers. We believed that we had developed a strong intervention programme based on…

  1. Opisthobranchs from the Lesser Antilles

    NARCIS (Netherlands)

    Bois-Reymond Marcus, Eveline du; Marcus, Ernst

    1963-01-01

    Thanks to help of the Government of the Netherlands, Dr. DIVA DINIZ CORRÊA, a lecturer in our Department, was able to work at the “Caraïbisch Marien-Biologisch Instituut” (Caribbean Marine Biological Institute; Carmabi) Curaçao, from January to July 1962. Besides actinians and nemerteans for her own

  2. Plaadid / Aleks Lange

    Index Scriptorium Estoniae

    Lange, Aleks

    2003-01-01

    Uutest plaatidest Ricky Martin "Almas del Silencio", Paul McCartney "Back In Th e World", The Ultimate "Diva Collection", Girls Aloud "Sound Of The Underground", Blacky "Kui me päikeseloojangut ootama peaks", Christoph Poppen, Münchner Kammerorchester, The Hilliard Ensemble, Cerys Matthews "Cockahoop", Radiohead "Hail to the Thief"

  3. Potential Benefits of Cattle Vaccination as a Supplementary Control for Bovine Tuberculosis

    Science.gov (United States)

    Conlan, Andrew J. K.; Brooks Pollock, Ellen; McKinley, Trevelyan J.; Mitchell, Andrew P.; Jones, Gareth J.; Vordermeier, Martin; Wood, James L. N.

    2015-01-01

    Vaccination for the control of bovine tuberculosis (bTB) in cattle is not currently used within any international control program, and is illegal within the EU. Candidate vaccines, based upon Mycobacterium bovis bacillus Calmette-Guérin (BCG) all interfere with the action of the tuberculin skin test, which is used to determine if animals, herds and countries are officially bTB-free. New diagnostic tests that Differentiate Infected from Vaccinated Animals (DIVA) offer the potential to introduce vaccination within existing eradication programs. We use within-herd transmission models estimated from historical data from Great Britain (GB) to explore the feasibility of such supplemental use of vaccination. The economic impact of bovine Tuberculosis for farmers is dominated by the costs associated with testing, and associated restrictions on animal movements. Farmers’ willingness to adopt vaccination will require vaccination to not only reduce the burden of infection, but also the risk of restrictions being imposed. We find that, under the intensive sequence of testing in GB, it is the specificity of the DIVA test, rather than the sensitivity, that is the greatest barrier to see a herd level benefit of vaccination. The potential negative effects of vaccination could be mitigated through relaxation of testing. However, this could potentially increase the hidden burden of infection within Officially TB Free herds. Using our models, we explore the range of the DIVA test characteristics necessary to see a protective herd level benefit of vaccination. We estimate that a DIVA specificity of at least 99.85% and sensitivity of >40% is required to see a protective benefit of vaccination with no increase in the risk of missed infection. Data from experimentally infected animals suggest that this target specificity could be achieved in vaccinates using a cocktail of three DIVA antigens while maintaining a sensitivity of 73.3% (95%CI: 61.9, 82.9%) relative to post

  4. Four-dimensional information visualization and analysis system

    Energy Technology Data Exchange (ETDEWEB)

    Rex, B.

    1994-09-01

    4DIVAS was conceived for two purposes. The first purpose was to fill the void between the current generation of GIS and high-end, image-only visualization systems. The gap existing between these two systems can be summarized as the limitation of relational database oriented GIS to deal with time and the poor connection in visualization systems to databases of any kind. The second purpose was to permit spatio-temporal modeling and query, functionalities currently underdeveloped in the commercial arena. At the Hanford Site, there are many potential applications for such a system, especially in the monitoring and modeling of potential constituent plumes in the subsurface hydrology over time, among others. In its current form, 4DIVAS exists as a proof-of-concept functional prototype and is therefore more of a technology than a product at this time.

  5. Construções e reconstruções: uma ressignificação do olhar para com as musas

    Directory of Open Access Journals (Sweden)

    Shirley Elias Vilela

    2011-06-01

    Full Text Available This work focuses on the ideological construction of female muses, beauty, consumer relations of this image and how it establishes itself in the social imaginary. Being a muse, diva, and movie star also meant becoming a myth. Many devices will make this diva in an almost supernatural being to the other’s eyes. Greta Garbo, Marlene Dietrich, Ava Gardner, Jane Russell, Brigitte Bardot and many others become professionals capable of incorporating screens of the immediate attraction. It is through several movies and visual media that builds a false democracy of beauty and the illusion that one day, ordinary people could also become benchmarks. Among these, one stands out for not being human. Barbie, a doll, appeared in a decade of extreme exaltation of feminine beauty and incorporated a little of each one of these beauties.

  6. AK-95血透机故障检修三例

    Institute of Scientific and Technical Information of China (English)

    田维良

    2006-01-01

    @@ 故障一:开机后,机器自检过程中出现红扳手,错误代码显示为C COFF 088 061. 检修过程:查错误代码手册提示该故障为DIVA/TAVA测试失败,因此一方面怀疑接头等可能会密封不严;另一方面阀密封不严.首先检查O型圈及透析器与旁路之间接头未发现异常.然后检查DIVA/TAVA/EVVA/REVA几只阀,最后查至REVA时发现其阀膜已经发白变硬,密封不严导致漏液.更换新阀膜后机器工作正常,故障排除.

  7. Anima Automata: On the Olympian Art of Song

    OpenAIRE

    Porzak, Simon Lucas

    2014-01-01

    Dominant explanations of the power of song, in musicology, sound studies, media theory, and our cultural mythologies about divas and pop singers, follow a Promethean trajectory: a singer wagers her originary humanity through an encounter with the machinery of music (vocal training, recording media, etc.); yet her song will finally carry an even more profound, immediate human meaning. Technology forms an accidental detour leading from humanity to more humanity. In an alternative, "Olympian" pr...

  8. Using Virtual Globes and a Java web Application to Visualize and Compare Ocean Observations and Model Data

    Science.gov (United States)

    Gemmell, A. L.; Blower, J.; Haines, K.; Smith, G.

    2007-12-01

    In order to better predict how the Earth's changing climate will affect ocean circulation, and more generally the behaviour of the ocean-atmosphere system, ocean modellers need to have the ability to accurately assimilate historical and near-real time data into their models. This process has traditionally included the use of fairly static plots of model and observed data in order to attempt to visualize where discrepancies between the two are greatest. Here we present OceanDIVA - Ocean Data Intercomparison and Visualization Application. OceanDIVA can read in ocean data from both local sources, and from any publicly accessible data holdings worldwide via OPeNDAP, and output the data into either Google Earth or a freely-available online virtual globe. One of its key capabilities is to read in model data from one source and observed data from another unrelated source, and to compare the two - giving data on the misfit. This is done in the form of colour-coded observation locations, or statistical difference plots averaged over regions which can be displayed on the virtual globe. If a particular profile is of interest then users may click on that icon and OceanDIVA will proceed to generate a plot of data with depth on the fly. Data may also be plotted on temperature levels which removes errors associated with estimating the depth at which water masses are found. By harvesting the power and ease of use of virtual globes, we see this as a useful tool to visualize and compare data from different sources, generating new datasets which have more value than the sum of their constituent parts. OceanDIVA could be readily expanded to incorporate, for example, satellite data, biological marine data, or data over land.

  9. Rescue of a vaccine strain of peste des petits ruminants virus: In vivo evaluation and comparison with standard vaccine.

    Science.gov (United States)

    Muniraju, Murali; Mahapatra, Mana; Buczkowski, Hubert; Batten, Carrie; Banyard, Ashley C; Parida, Satya

    2015-01-09

    Across the developing world peste des petits ruminants virus places a huge disease burden on agriculture, primarily affecting the production of small ruminant. The disease is most effectively controlled by vaccinating sheep and goats with live attenuated vaccines that provide lifelong immunity. However, the current vaccines and serological tests are unable to enable Differentiation between naturally Infected and Vaccinated Animals (DIVA). This factor precludes meaningful assessment of vaccine coverage and epidemiological surveillance based on serology, in turn reducing the efficiency of control programmes. The availability of a recombinant PPRV vaccine with a proven functionality is a prerequisite for the development of novel vaccines that may enable the development of DIVA tools for PPRV diagnostics. In this study, we have established an efficient reverse genetics system for PPRV Nigeria 75/1 vaccine strain and, further rescued a version of PPRV Nigeria 75/1 vaccine strain that expresses eGFP as a novel transcription cassette and a version of PPRV Nigeria 75/1 vaccine strain with mutations in the haemagglutinin (H) gene to enable DIVA through disruption of binding to H by the C77 monoclonal antibody used in the competitive (c) H-ELISA. All three rescued viruses showed similar growth characteristics in vitro in comparison to parent vaccine strain and, following in vivo assessment the H mutant provided full protection in goats. Although the C77 monoclonal antibody used in the cH-ELISA was unable to bind to the mutated form of H in vitro, the mutation was not sufficient to enable DIVA in vivo.

  10. [Intralobar pulmonary sequestration with multiple arterial blood supply].

    Science.gov (United States)

    Uroz Tristán, J; Mogueya, S A; Poenaru, D; Martínez Lagares, F; Arteaga García, R; Sanchís Solera, L; López-Pinto Ruiz, J

    1994-04-01

    We report the case of a 4 years old boy, who presented at our institution with reiterative neumonia affecting left basal lobe. Anomalous vascular appearance was detected in the chest x-ray. With the suspicion of pulmonary sequestration we carried on Digital Intravenous Angiography by Substraction (DIVAS) and aortogram. The anomalous systemic arterial supply was formed by 6 vessels coming from the thoracic aorta and going into the left lower lobe basal segment. Lobectomy was performed and previous diagnosis was confirmed pathologically.

  11. Plaadid / Villu Päärt

    Index Scriptorium Estoniae

    Päärt, Villu, 1972-

    2003-01-01

    Uutest plaatidest Sarah Connor "Unbelievable", Robbie Williams "Escapology", Stan Getz "The Definitive Stan Getz", Santana "Sunrise", Wallflowers "Red Letter Days", Robert Palmer "At His Very Best", INXS "Definitive", Hilary Hahn "Mendelssohn/Shostakovich", System Of A Down "Steal This Album!", Disturbed "Believe", "Divas Las Vegas", Yann Tiersen " C'Etait Ici", We Will Rock You " Original London Cast Recording", Busta Rhymes " It Ain't Safe No More", David Bowie "Best Of", Terence Trent D'Arby "Greatest Hits"

  12. Online Spatial Database of US Army Public Health Command Region-West Mosquito Surveillance Records: 1947-2009

    Science.gov (United States)

    2011-09-01

    geocoding errors. Output addresses were checked against input to identify discrepancies, and results that had a low precision level (to street or city...jwww.gpsvisuallzer.com/ geocoder / 30 http://www.cs.amedd.army.mil/dasqaDocuments.aspx?type=l THE ARMY MEDICAL DEPARTMENT JOURNAL combination of internet searches...calculated area. Data were filtered in Excel for unique locations, and these point data were converted to shape files for mapping in DIVA- GIS 5.2

  13. Il Fuoco or the fatal portrait : the XIXth century in the Italian silent cinema

    NARCIS (Netherlands)

    Blom, I.L.

    1992-01-01

    Dans les années dix de notre siècle, un genre nouveau de film s'installe en Italie aux côtés des grandes productions historiques : le cinéma des divas dont l'histoire-type tourne autour des drames d'un "ménage à trois". Il Fuoco (Giovanni Pastrone, 1915) serait, selon l'auteur, un film moderne qui,

  14. Eesti Ekspressi singel 3. Eesti Ekspressi singel 4

    Index Scriptorium Estoniae

    2008-01-01

    6. nov. ilmuva Eesti Ekspressi vahel on metal-bändi Metsatöll singel "Merehunt" lisalooga "Alle-aa". Metstöllu kontserdid: 11. nov. USAs Minneapolises festivalil Heathen Crusade Metalfest ja 21. nov. Tartu klubis Rock'n'Roll. 13. nov. ilmuva ajalehe vahel on ansambli HU? singel "Kuum (vulkaan, mis vahel puhkab)" lisalooga "Kosmos (Loop Piratesi remiks)". HU? kontserdid: 14. nov. Pärnus klubis Sugar, 21. nov. Tartus klubis Illusion ja 6. dets. Kuressaares klubis Diva

  15. Il Fuoco or the fatal portrait : the XIXth century in the Italian silent cinema

    NARCIS (Netherlands)

    Blom, I.L.

    1992-01-01

    Dans les années dix de notre siècle, un genre nouveau de film s'installe en Italie aux côtés des grandes productions historiques : le cinéma des divas dont l'histoire-type tourne autour des drames d'un "ménage à trois". Il Fuoco (Giovanni Pastrone, 1915) serait, selon l'auteur, un film moderne qui,

  16. Eesti Ekspressi singel 3. Eesti Ekspressi singel 4

    Index Scriptorium Estoniae

    2008-01-01

    6. nov. ilmuva Eesti Ekspressi vahel on metal-bändi Metsatöll singel "Merehunt" lisalooga "Alle-aa". Metstöllu kontserdid: 11. nov. USAs Minneapolises festivalil Heathen Crusade Metalfest ja 21. nov. Tartu klubis Rock'n'Roll. 13. nov. ilmuva ajalehe vahel on ansambli HU? singel "Kuum (vulkaan, mis vahel puhkab)" lisalooga "Kosmos (Loop Piratesi remiks)". HU? kontserdid: 14. nov. Pärnus klubis Sugar, 21. nov. Tartus klubis Illusion ja 6. dets. Kuressaares klubis Diva

  17. What you see (sonographically) is what you get: vein and patient characteristics associated with successful ultrasound-guided peripheral intravenous placement in patients with difficult access.

    Science.gov (United States)

    Panebianco, Nova L; Fredette, Jenna M; Szyld, Demian; Sagalyn, Emily B; Pines, Jesse M; Dean, Anthony J

    2009-12-01

    Ultrasound (US) has been shown to facilitate peripheral intravenous (IV) placement in emergency department (ED) patients with difficult IV access (DIVA). This study sought to define patient and vein characteristics that affect successful US-guided peripheral IV placement. This was a prospective observational study of US-guided IV placement in a convenience sample of DIVA patients in an urban, tertiary care ED. DIVA patients were defined as having any of the following: at least two failed IV attempts or a history of difficult access plus the inability to visualize or palpate any veins on physical exam. Patient characteristics (demographic information, vital signs, and medical history) were collected on enrolled patients. The relationships between patient characteristics, vein depth and diameter, US probe orientation, and successful IV placement were analyzed. A total of 169 patients were enrolled, with 236 attempts at access. Increasing vessel diameter was associated with a higher likelihood of success (odds ratio [OR] = 1.79 per 0.1-cm increase in vessel diameter, 95% confidence interval [CI] = 1.37 to 2.34). Increasing vessel depth did not affect success rates (OR = 0.96 per 0.1-cm increase of depth, 95% CI = 0.89 to 1.04) until a threshold depth of 1.6 cm, beyond which no vessels were successfully cannulated. Probe orientation and patient characteristics were unrelated to success. Success was solely related to vessel characteristics detected with US and not influenced by patient characteristics or probe orientation. Successful DIVA was primarily associated with larger vessel, while vessel depth up to >1.6 cm and patient characteristics were unrelated to success. Clinically, if two vessels are identified at a depth of <1.6 cm, the larger diameter vessel, even if comparatively deeper, should yield the greatest likelihood of success.

  18. The Anomalous Metalloporphyrin and Chlorophyll a Activated Chemiluminescence of Dimethyldioxetanone. Chemically Initiated Electron-Exchange Luminescence.

    Science.gov (United States)

    1980-02-26

    on the rate of reaction of decalin hydroperoxide . Moreover, the free-base reaction is not chemilumine- scent. More recently, McCapra reported...Sigma) was also purified by treatment with DDQ. Other metalloporphyrins were prepared by reaction of the appropriate diva- lent metal salt with the...Navy Annapolis, Maryland 21401 Washington, D.C. 20360 1 Naval Ocean Systems Center Defense Documentation Center Attn: Dr. S. Yamamoto , Marine Building

  19. Plaadid / Villu Päärt

    Index Scriptorium Estoniae

    Päärt, Villu, 1972-

    2003-01-01

    Uutest plaatidest Sarah Connor "Unbelievable", Robbie Williams "Escapology", Stan Getz "The Definitive Stan Getz", Santana "Sunrise", Wallflowers "Red Letter Days", Robert Palmer "At His Very Best", INXS "Definitive", Hilary Hahn "Mendelssohn/Shostakovich", System Of A Down "Steal This Album!", Disturbed "Believe", "Divas Las Vegas", Yann Tiersen " C'Etait Ici", We Will Rock You " Original London Cast Recording", Busta Rhymes " It Ain't Safe No More", David Bowie "Best Of", Terence Trent D'Arby "Greatest Hits"

  20. Recombinant M2e protein-based ELISA: a novel and inexpensive approach for differentiating avian influenza infected chickens from vaccinated ones.

    Directory of Open Access Journals (Sweden)

    Farhid Hemmatzadeh

    Full Text Available Available avian influenza (AIV serological diagnostic tests cannot distinguish vaccinated from naturally infected birds. Differentiation of vaccinated from infected animals (DIVA is currently advocated as a means of achieving the full control of H5N1. In this study, for the first time, recombinant ectodomain of M2 protein (M2e of avian influenza virus (H5N1 strain was used for the DIVA serology test. M2e was cloned into pMAL-P4X vector and expressed in E. coli cells. We used Western blot to recognize the expressed M2e-MBP protein by chicken antisera produced against live H5N1 virus. Also, the specificity of M2e-MBP protein was compared to the M2e synthetic peptide via ELISA. In M2e-MBP ELISA, all sera raised against the live avian influenza viruses were positive for M2e antibodies, whereas sera from killed virus vaccination were negative. Furthermore, M2e-MBP ELISA of the field sera obtained from vaccinated and non-vaccinated chickens showed negative results, while challenged vaccinated chickens demonstrated strong positive reactions. H5N1-originated recombinant M2e protein induced broad-spectrum response and successfully reacted with antibodies against other AIV strains such as H5N2, H9N2, H7N7, and H11N6. The application of the recombinant protein instead of synthetic peptide has the advantages of continues access to an inexpensive reagent for performing a large scale screening. Moreover, recombinant proteins provide the possibility of testing the DIVA results with an additional technique such a Western blotting which is not possible in the case of synthetic proteins. All together, the results of the present investigation show that recombinant M2e-MBP can be used as a robust and inexpensive solution for DIVA test.

  1. [Role of vaccination in animal health].

    Science.gov (United States)

    Pastoret, Paul-Pierre

    2012-03-01

    According to the IFAH, veterinary vaccines currently account for 26% of the global market in veterinary medicines, reflecting the importance of vaccines in animal health, as well as the number of wild and domesticated target species, and the monospecific nature of most vaccines. Multispecies vaccines include tetanus and rabies. In 2010, the number of food-producing animals was estimated to be roughly 20 billion and is rising gradually. Fowl currently represent the main food species. Veterinary vaccination has allowed the eradication of rinderpest, as officially declared last year (2011), jointly by the World Organisation for Animal Health (OIE) and the Food and Agriculture Organisation of the United Nations (FAO). Rinderpest was a real scourge, and was only the second viral disease to be totally eradicated (after human smallpox). One characteristic of veterinary vaccination is the DIVA approach, "differentiating infected from vaccinated animals". The DIVA strategy is especially interesting for regulated control of diseases like foot-and-mouth disease, infectious bovine rhinotracheitis, pseudorabies, and classical swine fever. DIVA vaccination requires prior serological testing. Vaccination is also used for wild animals such as foxes (rabies) and wild boars (classical swine fever). "In ovo" vaccination of fowl on day 18 of the incubation period is used to prevent Marek's disease for instance, and double vaccination (vector and insert) to prevent both Marek's disease and Gumboro's disease in fowl. Animal vaccination can also help to protect human health, as illustrated by fowl vaccination against salmonellosis.

  2. Development of a Blocking ELISA Using a Monoclonal Antibody to a Dominant Epitope in Non-Structural Protein 3A of Foot-and-Mouth Disease Virus, as a Matching Test for a Negative-Marker Vaccine

    Science.gov (United States)

    Fu, Yuanfang; Li, Pinghua; Cao, Yimei; Wang, Na; Sun, Pu; Shi, Qian; Ji, Xincheng; Bao, Huifang; Li, Dong; Chen, Yingli; Bai, Xingwen; Ma, Xueqing; Zhang, Jing; Lu, Zengjun; Liu, Zaixin

    2017-01-01

    Foot-and-mouth disease (FMD) is a devastating animal disease. Strategies for differentiation of infected from vaccinated animals (DIVA) remain very important for controlling disease. Development of an epitope-deleted marker vaccine and accompanying diagnostic method will improve the efficiency of DIVA. Here, a monoclonal antibody (Mab) was found to recognize a conserved “AEKNPLE” epitope spanning amino acids 109–115 of non-structural protein (NSP) 3A of foot-and-mouth disease virus (FMDV; O/Tibet/CHA/99 strain), which could be deleted by a reverse-genetic procedure. In addition, a blocking ELISA was developed based on this Mab against NSP 3A, which could serve as a matching test for a negative-marker vaccine. The criterion of this blocking ELISA was determined by detecting panels of sera from different origins. The serum samples with a percentage inhibition (PI) equal or greater than 50% were considered to be from infected animals, and those with <50% PI were considered to be from non-infected animals. This test showed similar performance when compared with other 2 blocking ELISAs based on an anti-NSP 3B Mab. This is the first report of the DIVA test for an NSP antibody based on an Mab against the conserved and predominant “AEKNPLE” epitope in NSP 3A of FMDV. PMID:28107470

  3. Control of swine pseudorabies in China: Opportunities and limitations.

    Science.gov (United States)

    Sun, Yuan; Luo, Yuzi; Wang, Chun-Hua; Yuan, Jin; Li, Na; Song, Kun; Qiu, Hua-Ji

    2016-02-01

    Pseudorabies (PR), also known as Aujeszky's disease (AD), is caused by pseudorabies virus (PRV) or called suid herpesvirus 1 (SuHV-1). It is an economically significant viral disease of pigs and other animals. Although the disease has been eradicated in commercial swine populations of some countries using gE-deleted vaccines and differentiating infected from vaccinated animals (DIVA) strategy, PR continues to be one of the most important diseases of pigs in many countries, particularly in regions with dense pig populations, including China. This article reviews the current situation of PR in China, including epidemiology, diagnostic assays, control strategies and challenges of the disease. PR has been endemic in most provinces of China largely due to the lack of appropriate compulsory vaccination campaigns of pigs, sufficient awareness and biosecurity measures, although gE-deleted vaccines based on the Bartha-K61 strain and regional DIVA-based eradication programs have been widely used in the past decades. Notably, since 2011, an emerging variant PRV with enhanced pathogenicity has become prevalent in vaccinated swine herds in many regions of China and the disease situation is worsening. Control and eventual eradication of PR remain a big challenge in China, and strengthened control measures based on updated DIVA strategy are urgently needed toward national eradication of PR.

  4. The topicality of pre-colonial Indonesian heroes: Recent popular fiction from Indonesia

    Directory of Open Access Journals (Sweden)

    Andre Syahreza

    2012-05-01

    Full Text Available Review of:Agus Sunyoto, Dhaeng Sekara: Telik sandi tanah pelik Majapahit. Yogyakarta: DIVA Press, 2010, 488 pp. ISBN 9786029782820. Price: IDR 55.000.Bagus Dilla, Bumi Sriwijaya. Yogyakarta: DIVA Press, 2010, 483 pp. ISBN 9786029782738. Price: IDR 58.000.Daryanto, Raden Fatah: Bara di atas Demak Bintara. Jakarta: Tiga Kelana, 2009, vi + 472 pp. ISBN 9786028535304.Enang Rokajat Asura, Prabu Siliwangi: Bara di balik terkoyaknya Raja Digdaya. Depok: Edelweiss, 2009, ix + 457 pp. ISBN 9789791962438.Enang Rokajat Asura, Wangsit Siliwangi: Harimau di tengah bara. Depok: Edelweiss, 2009, 444 pp. ISBN 9786028672009.Gamal Komandoko, Panembahan Senopati: Geger ramalan Sunan Giri. Yogyakarta: DIVA Press, 2009, 400 pp. ISBN 9789799637086.Langit Kresna Hariadi, Gajah Mada. Solo: Tiga Serangkai, 2004, x + 582 pp. ISBN 9796685582.Langit Kresna Hariadi, Gajah Mada: Bergelut dalam kemelut takhta dan angkara. Solo: Tiga Serangkai, 2006, xii + 508 pp. ISBN 9793301902.Langit Kresna Hariadi, Gajah Mada: Hamukti palapa. Solo: Tiga Serangkai, 2006, x + 694 pp. ISBN 9793303166.Langit Kresna Hariadi, Gajah Mada: Perang Bubat. Solo: Tiga Serangkai, 2006, xii + 448 pp. ISBN 9793304499.Langit Kresna Hariadi, Gajah Mada: Madakaripura Hamukti moksa. Solo: Tiga Serangkai, 2007, x + 582 pp. ISBN 9793307129.Nassirun Purwokartun, Penangsang: Tembang rindu dendam. Jakarta: Tiga Kelana, 2010, xvi + 704 pp. ISBN 786028535847.SW. Warsito and Harmadi, Airlangga. Yogyakarta: Flash Books, 2010, 389 pp. ISBN 9786029556735.

  5. Scientific Opinion on field trials for bovine tuberculosis vaccination

    Directory of Open Access Journals (Sweden)

    EFSA Panel on Animal Health and Welfare (AHAW

    2013-12-01

    Full Text Available The opinion provides advice relating to the design of field trials to test the performance of a vaccine for bovine tuberculosis (bTB, along with a test to Detect Infected among Vaccinated Animals (DIVA. The objective of cattle vaccination is to use the vaccine in combination with presently applied control measures within the EU as an aid towards bTB eradication. The ideal field trials for the DIVA test will follow the OIE guidelines for test validation. Any deviations from the ideal trial design in relation to DIVA test performance should be justified, and the bias that may subsequently be introduced should be accounted for. The ideal field trial design for vaccination performance should implement a double-blind randomised test scenario, and allow for known risk factors in the field situation. Any deviations from the ideal trial design in relation to vaccine performance should also be justified and bias that may subsequently be introduced should be accounted for. Relevant risk factors and possible confounders that should be taken into consideration in the design of field trials are described in this opinion. The safety of a candidate vaccine is guaranteed in the registration of a vaccine medication by a competent authority. The field trials will need to fulfil these requirements to prove that the use of this vaccine in the field is safe for both public health and the environment. Some additional remarks regarding the safety of this specific vaccine are included in this opinion.

  6. Characterization of Rift Valley fever virus MP-12 strain encoding NSs of Punta Toro virus or sandfly fever Sicilian virus.

    Directory of Open Access Journals (Sweden)

    Olga A Lihoradova

    Full Text Available Rift Valley fever virus (RVFV; genus Phlebovirus, family Bunyaviridae is a mosquito-borne zoonotic pathogen which can cause hemorrhagic fever, neurological disorders or blindness in humans, and a high rate of abortion in ruminants. MP-12 strain, a live-attenuated candidate vaccine, is attenuated in the M- and L-segments, but the S-segment retains the virulent phenotype. MP-12 was manufactured as an Investigational New Drug vaccine by using MRC-5 cells and encodes a functional NSs gene, the major virulence factor of RVFV which 1 induces a shutoff of the host transcription, 2 inhibits interferon (IFN-β promoter activation, and 3 promotes the degradation of dsRNA-dependent protein kinase (PKR. MP-12 lacks a marker for differentiation of infected from vaccinated animals (DIVA. Although MP-12 lacking NSs works for DIVA, it does not replicate efficiently in type-I IFN-competent MRC-5 cells, while the use of type-I IFN-incompetent cells may negatively affect its genetic stability. To generate modified MP-12 vaccine candidates encoding a DIVA marker, while still replicating efficiently in MRC-5 cells, we generated recombinant MP-12 encoding Punta Toro virus Adames strain NSs (rMP12-PTNSs or Sandfly fever Sicilian virus NSs (rMP12-SFSNSs in place of MP-12 NSs. We have demonstrated that those recombinant MP-12 viruses inhibit IFN-β mRNA synthesis, yet do not promote the degradation of PKR. The rMP12-PTNSs, but not rMP12-SFSNSs, replicated more efficiently than recombinant MP-12 lacking NSs in MRC-5 cells. Mice vaccinated with rMP12-PTNSs or rMP12-SFSNSs induced neutralizing antibodies at a level equivalent to those vaccinated with MP-12, and were efficiently protected from wild-type RVFV challenge. The rMP12-PTNSs and rMP12-SFSNSs did not induce antibodies cross-reactive to anti-RVFV NSs antibody and are therefore applicable to DIVA. Thus, rMP12-PTNSs is highly efficacious, replicates efficiently in MRC-5 cells, and encodes a DIVA marker, all of which

  7. Estimating ancestral distributions of lineages with uncertain sister groups: a statistical approach to Dispersal-Vicariance Analysis and a case using Aesculus L. (Sapindaceae) including fossils

    Institute of Scientific and Technical Information of China (English)

    A.J. HARRIS; Qiu-Yun (Jenny) XIANG

    2009-01-01

    We propose a simple statistical approach for using Dispersal-Vicariance Analysis (DIVA) software to infer biogeographic histories without fully bifurcating trees. In this approach, ancestral ranges are first optimized for a sample of Bayesian trees. The probability P of an ancestral range r at a node is then calculated as P(rY)= Σnt=1 F(rY)tPt where Y is a node, and F(rY) is the frequency of range r among all the optimal solutions resulting from DIVA optimization at node Y, t is one of n topologies optimized, and Pt is the probability of topology t. Node Y is a hypothesized ancestor shared by a specific crown lineage and the sister of that lineage "x", where x may vary due to phylogenetic uncertainty (polytomies and nodes with posterior probability <100%). Using this method, the ancestral distribution at Y can be estimated to provide inference of the geographic origins of the specific crown group of interest. This approach takes into account phylogenetic uncertainty as well as uncertainty from DIVA optimization. It is an extension of the previously described method called Bayes-DIVA, which pairs Bayesian phylogenetic analysis with biogeographic analysis using DIVA. Further, we show that the probability P of an ancestral range at Y calculated using this method does not equate to pp* F(rY) on the Bayesian consensus tree when both variables are < 100%, where pp is the posterior probability and F(rY) is the frequency of range r for the node containing the specific crown group. We tested our DIVA-Bayes approach using Aesculus L., which has major lineages unresolved as a polytomy. We inferred the most probable geographic origins of the five traditional sections of Aesculus and ofAesculus californica Nutt. and examined range subdivisions at parental nodes of these lineages.Additionally, we used the DIVA-Bayes data from Aesculus to quantify the effects on biogeographic inference of including two wildcard fossil taxa in phylogenetic analysis. Our analysis resolved the

  8. Two new and one known species of Tergestia Stossich, 1899 (Trematoda: Fellodistomidae) with novel molecular characterisation for the genus.

    Science.gov (United States)

    Wee, Nicholas Q-X; Cutmore, Scott C; Yong, Russell Q-Y; Cribb, Thomas H

    2017-09-02

    Combined morphological and molecular analyses are employed to characterise three species of Tergestia Stossich, 1899 (Digenea: Fellodistomidae) from fishes of Moreton Bay, Queensland, Australia. Tergestia clonacantha Manter, 1963 is reported here for the first time from the halfbeak (Beloniformes: Hemiramphidae) species Arrhamphus sclerolepis krefftii (Steindachner), Hyporhamphus australis (Steindachner), H. quoyi (Valenciennes) and H. regularis ardelio (Whitley). Two new species, both infecting trevally (Perciformes: Carangidae) species, are described: T. maryae n. sp. from Alepes apercna Grant and T. henryi n. sp. from Pantolabus radiatus (MacLeay). Complete ITS2 and partial 28S ribosomal DNA data were generated for each of the new taxa. The three species differ from each other by 47-58 base pairs (bp) in the ITS2 rDNA region. Phylogenetic analysis of 28S rDNA supports Tergestia as a reliable generic concept, with our analyses showing that some species of the genus form a well-supported clade to the exclusion of all other fellodistomids for which sequence data are available.

  9. Identification of Fungus Flora Associated with Lagenaria Siceraria (Molina Standl in Côte d’Ivoire

    Directory of Open Access Journals (Sweden)

    Koffi Ahébé Marie-Hélène

    2013-10-01

    Full Text Available Lagenaria siceraria (Molina Standl is a cucurbit which seeds are consumed by people in rural and urban Africa. This plant is subjected to a strong parasitic and diseases pressure that reduces seeds production. Efficient fight against plant parasite, particularly fungus is a prerequisite for an improved productivity. This study was undertaken in five localities (Alepe, Bondoukou, Bongouanou, Divo and Korhogo belonging to three agroecological areas of Côte d’Ivoire. The aim was to identify fungal genera infecting L. siceraria in order to design an efficient control measure. Leaf samples with necrosis and discoloration symptoms were collected throughout the localities and subsequently, fungus were isolated and identified in laboratory. From a total of 750 samples collected, 7 types of symptoms were distinguished. Fungal genera found in all of the localities were Aspergillus, Botryosphaeria, Cochliobolus, Colletotrichum, Fusarium, Lasiodiplodia and Phoma. Only Pestalotiopsis was specific to the locality of Divo. An ANOVA test performed on the data showed a significant difference between fungal genera in terms of isolation frequency. Principal components analysis revealed that fungus distribution in each locality was correlated with climatic factors.

  10. Safety and Efficacy Profile of Commercial Veterinary Vaccines against Rift Valley Fever: A Review Study

    Science.gov (United States)

    2016-01-01

    Rift Valley Fever (RVF) is an infectious illness with serious clinical manifestations and health consequences in humans as well as a wide range of domestic ruminants. This review provides significant information about the prevention options of RVF along with the safety-efficacy profile of commercial vaccines and some of RVF vaccination strategies. Information presented in this paper was obtained through a systematic investigation of published data about RVF vaccines. Like other viral diseases, the prevention of RVF relies heavily on immunization of susceptible herds with safe and cost-effective vaccine that is able to confer long-term protective immunity. Several strains of RVF vaccines have been developed and are available in commercial production including Formalin-Inactivated vaccine, live attenuated Smithburn vaccine, and the most recent Clone13. Although Formalin-Inactivated vaccine and live attenuated Smithburn vaccine are immunogenic and widely used in prevention programs, they proved to be accompanied by significant concerns. Despite Clone13 vaccine being suggested as safe in pregnant ewes and as highly immunogenic along with its potential for differentiating infected from vaccinated animals (DIVA), a recent study raised concerns about the safety of the vaccine during the first trimester of gestation. Accordingly, RVF vaccines that are currently available in the market to a significant extent do not fulfill the requirements of safety, potency, and DIVA. These adverse effects stressed the need for developing new vaccines with an excellent safety profile to bridge the gap in safety and immunity. Bringing RVF vaccine candidates to local markets besides the absence of validated serological test for DIVA remain the major challenges of RVF control. PMID:27689098

  11. Safety and Efficacy Profile of Commercial Veterinary Vaccines against Rift Valley Fever: A Review Study

    Directory of Open Access Journals (Sweden)

    Moataz Alhaj

    2016-01-01

    Full Text Available Rift Valley Fever (RVF is an infectious illness with serious clinical manifestations and health consequences in humans as well as a wide range of domestic ruminants. This review provides significant information about the prevention options of RVF along with the safety-efficacy profile of commercial vaccines and some of RVF vaccination strategies. Information presented in this paper was obtained through a systematic investigation of published data about RVF vaccines. Like other viral diseases, the prevention of RVF relies heavily on immunization of susceptible herds with safe and cost-effective vaccine that is able to confer long-term protective immunity. Several strains of RVF vaccines have been developed and are available in commercial production including Formalin-Inactivated vaccine, live attenuated Smithburn vaccine, and the most recent Clone13. Although Formalin-Inactivated vaccine and live attenuated Smithburn vaccine are immunogenic and widely used in prevention programs, they proved to be accompanied by significant concerns. Despite Clone13 vaccine being suggested as safe in pregnant ewes and as highly immunogenic along with its potential for differentiating infected from vaccinated animals (DIVA, a recent study raised concerns about the safety of the vaccine during the first trimester of gestation. Accordingly, RVF vaccines that are currently available in the market to a significant extent do not fulfill the requirements of safety, potency, and DIVA. These adverse effects stressed the need for developing new vaccines with an excellent safety profile to bridge the gap in safety and immunity. Bringing RVF vaccine candidates to local markets besides the absence of validated serological test for DIVA remain the major challenges of RVF control.

  12. Multimeric recombinant M2e protein-based ELISA: a significant improvement in differentiating avian influenza infected chickens from vaccinated ones.

    Directory of Open Access Journals (Sweden)

    Farshid Hadifar

    Full Text Available Killed avian influenza virus (AIV vaccines have been used to control H5N1 infections in countries where the virus is endemic. Distinguishing vaccinated from naturally infected birds (DIVA in such situations however, has become a major challenge. Recently, we introduced the recombinant ectodomain of the M2 protein (M2e of H5N1 subtype as a novel tool for an ELISA based DIVA test. Despite being antigenic in natural infection the monomer form of the M2e used in ELISA had limited antigenicity and consequently poor diagnostic capability. To address this shortcoming, we evaluated the use of four tandem copies of M2e (tM2e for increased efficiency of M2e antibody detection. The tM2e gene of H5N1 strain from Indonesia (A/Indonesia/CDC540/2006 was cloned into a pMAL- p4x expression vector and expressed in E.coli as a recombinant tM2e-MBP or M2e-MBP proteins. Both of these, M2e and tM2e antigens reacted with sera obtained from chickens following live H5N1 infection but not with sera from vaccinated birds. A significantly stronger M2e antibody reaction was observed with the tM2e compared to M2e antigen. Western blotting also supported the superiority of tM2e over M2e in detection of specific M2e antibodies against live H5N1 infection. Results from this study demonstrate that M2e tetramer is a better antigen than single M2e and could be more suitable for an ELISA based DIVA test.

  13. The ladies trial: laparoscopic peritoneal lavage or resection for purulent peritonitisA and Hartmann's procedure or resection with primary anastomosis for purulent or faecal peritonitisB in perforated diverticulitis (NTR2037

    Directory of Open Access Journals (Sweden)

    Bruin Sjoerd C

    2010-10-01

    Full Text Available Abstract Background Recently, excellent results are reported on laparoscopic lavage in patients with purulent perforated diverticulitis as an alternative for sigmoidectomy and ostomy. The objective of this study is to determine whether LaparOscopic LAvage and drainage is a safe and effective treatment for patients with purulent peritonitis (LOLA-arm and to determine the optimal resectional strategy in patients with a purulent or faecal peritonitis (DIVA-arm: perforated DIVerticulitis: sigmoidresection with or without Anastomosis. Methods/Design In this multicentre randomised trial all patients with perforated diverticulitis are included. Upon laparoscopy, patients with purulent peritonitis are treated with laparoscopic lavage and drainage, Hartmann's procedure or sigmoidectomy with primary anastomosis in a ratio of 2:1:1 (LOLA-arm. Patients with faecal peritonitis will be randomised 1:1 between Hartmann's procedure and resection with primary anastomosis (DIVA-arm. The primary combined endpoint of the LOLA-arm is major morbidity and mortality. A sample size of 132:66:66 patients will be able to detect a difference in the primary endpoint from 25% in resectional groups compared to 10% in the laparoscopic lavage group (two sided alpha = 5%, power = 90%. Endpoint of the DIVA-arm is stoma free survival one year after initial surgery. In this arm 212 patients are needed to significantly demonstrate a difference of 30% (log rank test two sided alpha = 5% and power = 90% in favour of the patients with resection with primary anastomosis. Secondary endpoints for both arms are the number of days alive and outside the hospital, health related quality of life, health care utilisation and associated costs. Discussion The Ladies trial is a nationwide multicentre randomised trial on perforated diverticulitis that will provide evidence on the merits of laparoscopic lavage and drainage for purulent generalised peritonitis and on the optimal resectional strategy

  14. Aprovechamiento metabólico de granos de sorgo entero con diferentes contenidos de taninos en novillos británicos

    Directory of Open Access Journals (Sweden)

    A. E Fernández

    Full Text Available Se evaluó la digestibilidad in vivo del almidón (DIVA del grano de sorgo entero, con altos y bajos contenidos de taninos (GSAT y GSBT, respectivamente, como suplemento, en machos bovinos en crecimiento; así como las pérdidas en heces y el efecto diferencial de estas pérdidas, según el contenido de taninos. Se establecieron dos tratamientos (T1 y T2 y dos repeticiones. Se empleó un diseño totalmente aleatorizado y cada animal constituyó una unidad experimental. Las dietas se suministraron una vez al día y estaban compuestas por diferentes proporciones de GSAT y GSBT, pellet de harina de girasol y heno de pastura mixta. El nivel proteínico fue de 18 y 15 % para el T1 y el T2, respectivamente. Se midió el consumo de MS (CMS, la digestibilidad in vivo de la MS (DIVMS, la DIVA y el consumo de agua por kilogramo de MS consumida. El CMS fue de 8,5 y 12,0 kg para el T1 y el T2, respectivamente, mientras que la DIVMS fue similar (71,64 y 71,66 %. La DIVA fue mayor en T1 (74,25 vs. 66,67 %; mientras que la pérdida de almidón en heces lo fue en T2 (31,56 vs. 27,27 %. El consumo de agua fue similar (1,8 L de agua/ kg de MS. Se concluye que hubo un comportamiento diferenciado en el aprovechamiento del grano de sorgo entero, debido al tamaño del animal y a los diferentes contenidos de taninos. Los terneros aprovecharon el 33 % de la oferta, y los novillos, el 25 %.

  15. The challenges of classical swine fever control: modified live and E2 subunit vaccines.

    Science.gov (United States)

    Huang, Yu-Liang; Deng, Ming-Chung; Wang, Fun-In; Huang, Chin-Cheng; Chang, Chia-Yi

    2014-01-22

    Classical swine fever (CSF) is an economically important, highly contagious disease of swine worldwide. CSF is caused by classical swine fever virus (CSFV), and domestic pigs and wild boars are its only natural hosts. The two main strategies used to control CSF epidemic are systematic prophylactic vaccination and a non-vaccination stamping-out policy. This review compares the protective efficacy of the routinely used modified live vaccine (MLV) and E2 subunit vaccines and summarizes the factors that influence the efficacy of the vaccines and the challenges that both vaccines face to CSF control. Although MLV provide earlier and more complete protection than E2 subunit vaccines, it has the drawback of not allowing differentiation between infected and vaccinated animals (DIVA). The marker vaccine of E2 protein with companion discriminatory test to detect antibodies against E(rns) allows DIVA and is a promising strategy for future control and eradication of CSF. Maternal derived antibody (MDA) is the critical factor in impairing the efficacy of both MLV and E2 subunit vaccines, so the well-designed vaccination programs of sows and piglets should be considered together. Because of the antigen variation among various genotypes of CSFV, antibodies raised by either MLV or subunit vaccine neutralize genotypically homologous strains better than heterologous ones. However, although this is not a major concern for MLV as the induced immune responses can protect pigs against the challenge of various genotypes of CSFVs, it is critical for E2 subunit vaccines. It is thus necessary to evaluate whether the E2 subunit vaccine can completely protect against the current prevalent strains in the field. An ideal new generation of vaccine should be able to maintain the high protective efficiency of MLV and overcome the problem of antigenic variations while allowing for DIVA.

  16. The innovations with the medical applications of white LEDs and the breakthrough for new business

    Science.gov (United States)

    Shimada, Jun-ichi; Itoh, Kazuhiro; Nishimura, Motohiro; Kawakami, Youichi; Tsuji, Kiyotsugu

    2006-02-01

    The distance between the LED and the surface of the target organ is about 4-5 cm, and we think this will become the "ultimate super-localized LED lighting". In an experiment with swine, we placed a LED module at the tip of the retractor. When compared to endoscopic lighting, this method illuminated the entire thoracic cavity more brightly. Since the light is emitted from the cylinder-shaped camera component, the light is unidirectional, and the shadows from the surgical instruments are moved to the side of the incision. Retractor LED lights provided enough light in the thoracic cavity. we believe that "medical white LEDs" can contribute in clinical settings as a light source for performing safe operations with bright surgical fields in the near future. Also, we use our LEDs for new real business. In the summer of 2004, LED lighting was world first used in the 1200 year-old Gion Festival for the first time in history as "a lighting device that does not destroy cultural assets by light heat". And the next is "Lighting at the "Diva status at diva gate" and the "Thousand Armed Avalokiteshwara in innermost sanctuary in the main hall" at Kiyomizudera in Kyoto". It was a great success, and we were invited back in the spring of 2005 and for future applications. We think this is the first real application of LEDs as an outdoor lighting device. The number of people who visit Kiyomizudera is 4000,000 annually, and LEDs were adopted to illuminate the diva gate.

  17. Something different - caching applied to calculation of impedance matrix elements

    CSIR Research Space (South Africa)

    Lysko, AA

    2012-09-01

    Full Text Available , Software and User's Manual?, Artech House, 2002. [6] S.N. Makarov, ?Antenna an EM Modeling with Matlab?, John Wiley & Sons, 2002, 288 pages. [7] B.M. Kolundzija et al., ?WIPL-D Pro v6.1: 3D Electromagnetic Solver, Professional Edition. User?s Manual... and Their Application to Wire Radiators?, Doctoral thesis, NTNU-trykk, Norwegian University of Science and Technology, 2010, 291 pages. Electronic file available at http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu :diva-7872 [27] B.M. Kolundzija, and B.D. Popovic...

  18. Josephine Baker: A Chanteuse and a Fighter

    Directory of Open Access Journals (Sweden)

    Konomi Ara

    2010-03-01

    Full Text Available This excerpt is from her newly-published biography of Josephine Baker, “A Fighting Diva.” It tells the intriguing story of Baker’s travels to Japan, her close friendship with the Japanese humanitarian Miki Sawada, and her adoption of a pair of Japanese orphans. Even after she achieved celebrity in France, Baker’s experience as a Black American led her to develop an antiracist philosophy at a worldwide level, and she combined political militancy in the public sphere with a personal commitment through the formation of an international multiracial household of children, the “Rainbow Tribe.”

  19. Josephine Baker: A Chanteuse and a Fighter

    Directory of Open Access Journals (Sweden)

    Konomi Ara

    2010-03-01

    Full Text Available

    This excerpt is from her newly-published biography of Josephine Baker, “A Fighting Diva.” It tells the intriguing story of Baker’s travels to Japan, her close friendship with the Japanese humanitarian Miki Sawada, and her adoption of a pair of Japanese orphans. Even after she achieved celebrity in France, Baker’s experience as a Black American led her to develop an antiracist philosophy at a worldwide level, and she combined political militancy in the public sphere with a personal commitment through the formation of an international multiracial household of children, the “Rainbow Tribe.”

  20. Synthesis of Polymers Containing Covalently Bonded NLO Chromophores

    Science.gov (United States)

    Denga, Xiao-Hua; Sanghadasa, Mohan; Walton, Connie; Penn, Benjamin B.; Amai, Robert L. S.; Clark, Ronald D.

    1998-01-01

    Polymers containing covalently bonded nonlinear optical (NLO) chromophores are expected to possess special properties such as greater stability, better mechanical processing, and easier film formation than their non-polymeric equivalent. For the present work, polymethylmethacrylate (PMMA) was selected as the basic polymer unit on which to incorporate different NLO chromophores. The NLO components were variations of DIVA {[2-methoxyphenyl methylidene]-propanedinitrile} which we prepared from vanillin derivatives and malononitrile. These were esterified with methacrylic acid and polymerized either directly or with methyl methacrylate to form homopolymers or copolymers respectively. Characterization of the polymers and NLO property studies are underway.

  1. New interpretations of fluvial sediments from the Kras

    Directory of Open Access Journals (Sweden)

    Andrej Mihevc

    2007-12-01

    Full Text Available Important unroofed caves with fluvial sediments from Divaški kras, Matarsko podolje and Podgorski kras are presented. Extend of the phenomena and relation to the existing caves and karst surface and geomorphological meaning of them are described. Sediments from them were analysed and dated with different methods. The largest age of the sediment was found in the unroofed cave excavated in Črnotiče quarry. In the cave wall fossil remains of stygobiont Marifugia cavatica were covered by 3.2-4.1 Ma old fluvial sediments.

  2. Development of a Façade Assessment and Design Tool for Solar Energy (FASSADES)

    OpenAIRE

    Jouri Kanters; Maria Wall; Marie-Claude Dubois

    2014-01-01

    Planning energy-efficient buildings which produce on-site renewable energy in an urban context is a challenge for all involved actors in the planning process. The primary objective of this study was to develop a façade assessment and design tool for solar energy (FASSADES) providing the necessary information for all stakeholders in the design process. The secondary objective was to demonstrate the tool by performing an assessment analysis of a building block. The FASSADES tool is a DIVA4Rhino...

  3. Biogeografía histórica de la familia Curculionidae (Coleoptera en las subregiones Subantártica y Chilena Central Historical biogeography of family Curculionidae (Coleoptera on Subantarctic and Central Chilean subregions

    Directory of Open Access Journals (Sweden)

    Paula Posadas

    2003-07-01

    Full Text Available El análisis de los patrones de distribución y la información filogenética de 71 especies de Curculionidae, distribuidas en las subregiones Subantártica y Chilena Central, aplicando los métodos biogeográficos de los árboles reconciliados y análisis de parsimonia de Brooks (BPA, resulta en un único cladograma general de áreas: ((Chile Central (Maule, Bosque Valdiviano (Islas Malvinas (Páramo Magallánico, Bosque Magallánico. La aplicación del análisis de dispersión-vicarianza (DIVA muestra que el evento vicariante más frecuente es el que separa las Islas Malvinas de las otras dos provincias subantárticas meridionales. Por otra parte, de acuerdo con los resultados del DIVA, se evidencia que las relaciones entre el Maule, Bosque Valdiviano y Chile Central no se deberían a eventos de vicarianza sino a eventos de dispersión, ya que el 61% del total de los eventos de dispersión implica a estas tres áreas. Aún más, los eventos de dispersión más frecuentes implican al Maule, ya sea en relación con Chile Central o con el Bosque Valdiviano, por lo que se deduce que la aparente complejidad de las relaciones biogeográficas del Maule podría estar relacionada con dichos eventos de dispersión.The application of the historical biogeographic techniques of reconciled trees and Brooks parsimony analysis (BPA to the distributional and phylogenetic information of 71 species of Curculionidae ranged in the Subantarctic and Central Chilean subregions results in a single general area cladogram: ((Central Chile (Maule, Valdivian Forest (Malvinas Islands (Magellanic Moorland, Magellanic Forest. The application of dispersal-vicariance analysis (DIVA to the same data set shows as the most frequent vicariant event the split of Islas Malvinas from the two southern Subantarctic provinces. In addition, DIVA results evidenced that the relationship among Maule, Valdivian Forest, and Central Chile did not arise from vicariance events (common history

  4. Engajamento e intervenção sonora no Brasil no pós-1964: a ditadura militar e os sentidos plurais do show Opinião

    Directory of Open Access Journals (Sweden)

    Kátia Rodrigues Paranhos

    2012-04-01

    Full Text Available This paper deals with the historical relevance of Opinião, a play enacted in December 1964, which had an important role as a political representation of the resistance to the military dictatorship in Brazil. I highlight some key aspects of this musical, such as the mixture of cultural traditions, the predominance of what Eric Hobsbawm names “functional songs” (labor songs, satirical music and love regrets as well as the art production/creation of actors/singers Nara Leão (a Bossa Nova diva, João do Vale (a Northeastern composer and Zé Keti (a Rio de Janeiro samba composer.

  5. Acumulação de matéria seca do tomateiro cultivado em substrato com diferentes doses de fertilizantes Dry matter accumulation of tomato plants grown in substrate under different fertilizer doses

    OpenAIRE

    2001-01-01

    Plantas de tomateiro foram cultivadas em substrato no interior de um ambiente protegido localizado no Departamento de Fitotecnia da UFSM, em Santa Maria, RS, com o objetivo de determinar o efeito de cinco doses de fertilizantes sobre a acumulação da matéria seca da parte vegetativa e dos frutos. Foram efetuados três experimentos, no outono-inverno de 1997, na primavera-verão de 1997 e no outono-inverno de 1998, empregando os híbridos Diva, Monte Carlo e Saladette, respectivamente. Foi utiliza...

  6. Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars

    DEFF Research Database (Denmark)

    Widén, F.; Everett, H.; Blome, S.

    2014-01-01

    Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated...... animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event...

  7. High speed cine film studies of plasma behaviour and plasma surface interactions in tokamaks

    Energy Technology Data Exchange (ETDEWEB)

    Goodall, D.H.J. (Euratom/UKAEA Fusion Association, Abingdon (UK). Culham Lab.)

    High speed cine photography is a useful diagnostic aid for studying plasma behaviour and plasma surface interactions. Several workers have filmed discharges in tokamaks including ASDEX, DITE, DIVA, ISX, JFT2, TFR and PLT. These films are discussed and examples given of the observed phenomena which include plasma limiter interactions, diverted discharges, disruptions, magnetic islands and moving glowing objects often known as 'UFOs'. Examples of plasma structures in ASDEX and DITE not previously published are also given. The paper also reports experiments in DITE to determine the origin of UFOs.

  8. Immaterial labour, collaborative cultural production and the economy of the gift

    OpenAIRE

    Clóvis Ricardo Montenegro de Lima; Daniella Pizarro; Elisangela Faustino; Maireli Dittrich

    2009-01-01

    Resumo Neste artigo discutem-se as relações entre trabalho imaterial, produção cultural colaborativa e  economia da dádiva na sociedade atual. O intelecto geral é relacionado com a inteligência coletiva das redes digitais. A produção colaborativa emerge como forma privilegiada do fazer artístico e cultural. Esta produção pode contribuir para promoção e preservação da diversidade cultural. A produção cultural colaborativa evidencia pluralismo econômico. A generalização do trabalho imaterial pr...

  9. Survival of crimes of suspicion: the case of article 259 of the Argentine Penal Code

    OpenAIRE

    2011-01-01

    Los “delitos de sospecha”, se encuentran plenamente vigentes pese a ser tipos manifiestamente inconstitucionales por violar los principios de inocencia, culpabilidad, lesividad, legalidad y de acto. Para demostrar tal afirmación, luego de caracterizar a esta categoría, se verifican sus elementos analizando los delitos de admisión y ofrecimiento de dádivas a funcionarios públicos del artículo 259 del Código penal argentino. The “crimes of suspicion”, are effective totally in spite...

  10. EXISTENCE OF POSITIVE SOLUTIONS FOR A CLASS OF PARABOLIC EQUATIONS WITH NATURAL GROWTH TERMS AND L1 DATA

    Institute of Scientific and Technical Information of China (English)

    Kaouther AMMAR; Hicham REDWANE

    2014-01-01

    We study a class of nonlinear parabolic equations of the type:∂b(u)∂t -diva(x, t, u)∇u+g(u)|∇u|2=f, where the right hand side belongs to L1(Q), b is a strictly increasing C1-function and-div(a(x, t, u)∇u) is a Leray-Lions operator. The function g is just assumed to be con-tinuous on R and to satisfy a sign condition. Without any additional growth assumption on u, we prove the existence of a renormalized solution.

  11. Primeiro os amigos: os aniversários da infância, dar e receber Friends first: childhood birthdays, giving and receiving

    Directory of Open Access Journals (Sweden)

    Régine Sirota

    2005-08-01

    Full Text Available Desde a infância, os aniversários propiciam trabalhos de socialização. Esse ritual, que costuma agrupar crianças da mesma faixa etária, faz surgir uma obrigação de trocar presentes: dádivas e contradádivas regem as seqüências da festa de aniversário. A abordagem etnográfica permite apreender o papel que cada ator social (filhos, pais, amigos desempenha na construção social desse ritual. Os presentes estão no cerne de um verdadeiro trabalho de negociações no qual se confrontam constantemente normas e valores.Ever since childhood birthday parties allow a process of socialization. Such ritual, which usually gathers children of a same age group, is an opportunity to exchange presents: gifts and counter-gifts structure the sequences of birthday parties. An ethnological approach thus enables to seize the contribution of each social actor (children, parents, relatives, and friends in the social constructions of this ritual. Presents are the object of a real negotiation implying a permanent confrontation of norms and values through which a social link is built.

  12. Towards a global historical biogeography of Palms

    Science.gov (United States)

    Couvreur, Thomas; Baker, William J.; Frigerio, Jean-Marc; Sepulchre, Pierre; Franc, Alain

    2017-04-01

    Four mechanisms are at work for deciphering historical biogeography of plants : speciation, extinction, migration, and drift (a sort of neutral speciation). The first three mechanisms are under selection pressure of the environment, mainly the climate and connectivity of land masses. Hence, an accurate history of climate and connectivity or non connectivity between landmasses, as well as orogenesis processes, can shed new light on the most likely speciation events and migration routes driven by paleogeography and paleoclimatology. Currently, some models exist (like DIVA) to infer the most parsimonious history (in the number of migration events) knowing the speciation history given by phylogenies (extinction are mostly unknown), in a given setting of climate and landmass connectivity. In a previous project, we have built in collaboration with LSCE a series of paleogeographic and paleoclimatic maps since the Early Cretaceous. We have developed a program, called Aran, which enables to extend DIVA to a time series of varying paleoclimatic and paleogeogarphic conditions. We apply these new methods and data to unravel the biogeographic history of palms (Arecaceae), a pantropical family of 182 genera and >2600 species whose divergence is dated in Late Cretaceous (100 My). Based on a robust dated molecular phylogeny, novel paleoclimatic and paleogeographic maps, we will generate an updated biogeographic history of Arecaceae inferred from the most parsimonious history using Aran. We will discuss the results, and put them in context with what is known and needed to provide a global biogeographic history of tropical palms.

  13. Evaluation of architectural paradigms for addressing theprocessor-memory gap

    Energy Technology Data Exchange (ETDEWEB)

    Oliker, Leonid; Gorden, Grime; Husbands, Parry; Chame, Jacqualine

    2003-07-04

    Many high performance applications run well below the peak arithmetic performance of the underlying machine, with inefficiencies often attributed to poor memory system behavior. In the context of scientific computing we examine three emerging processors designed to address the well-known gap between processor and memory performance through the exploitation of data parallelism. The VIRAM architecture uses novel PIM technology to combine embedded DRAM with a vector co-processor for exploiting its large bandwidth potential. The DIVA architecture incorporates a collection of PIM chips as smart-memory coprocessors to a conventional microprocessor, and relies on superword-level parallelism to make effective use of the available memory bandwidth. The Imagine architecture provides a stream-aware memory hierarchy to support the tremendous processing potential of SIMD controlled VLIW clusters. First we develop a scalable synthetic probe that allows us to parametize key performance attributes of VIRAM, DIVA and Imagine while capturing the performance crossover points of these architectures. Next we present results for scientific kernels with different sets of computational characteristics and memory access patterns. Our experiments allow us to evaluate the strategies employed to exploit data parallelism, isolate the set of application characteristics best suited to each architecture and show a promising direction towards interfacing leading-edge processor technology with high-end scientific computations.

  14. Current perspectives on conventional and novel vaccines against peste des petits ruminants.

    Science.gov (United States)

    Liu, Fuxiao; Wu, Xiaodong; Liu, Wenhua; Li, Lin; Wang, Zhiliang

    2014-12-01

    Peste des petits ruminants (PPR) is an acute or subacute, highly contagious viral disease of small ruminants, characterized by fever, oculonasal discharges, stomatitis, diarrhoea and pneumonia. This disease is included in the OIE (Office International des Epizooties) list of notifiable terrestrial animal diseases. PPR was first described in the early 1940s in Côte d'Ivoire, and at present, PPR is mainly circulating in Western and Central Africa, the Arabian Peninsula and Southern Asia. Peste des petits ruminants virus (PPRV), the etiological agent of PPR, is classified into the genus Morbillivirus in the family Paramyxoviridae, as its biological and physicochemical features are closely related to the other morbilliviruses. The first homologous PPR vaccine was developed by an artificially attenuated PPRV, named as Nigeria 75/1, which has been widely used in the production of live attenuated vaccines to protect small ruminants. A new generation of PPR vaccine candidates can be genetically modified to differentiate infected from vaccinated animals (DIVA), which nevertheless is difficult to achieve by conventional vaccines. In this review, we systematically discussed a broad range of vaccines against PPR, including commercially available vaccines and potential vaccine candidates, and further DIVA strategies for immunization with the new generation vaccines.

  15. Web-based visualization of gridded dataset usings OceanBrowser

    Science.gov (United States)

    Barth, Alexander; Watelet, Sylvain; Troupin, Charles; Beckers, Jean-Marie

    2015-04-01

    OceanBrowser is a web-based visualization tool for gridded oceanographic data sets. Those data sets are typically four-dimensional (longitude, latitude, depth and time). OceanBrowser allows one to visualize horizontal sections at a given depth and time to examine the horizontal distribution of a given variable. It also offers the possibility to display the results on an arbitrary vertical section. To study the evolution of the variable in time, the horizontal and vertical sections can also be animated. Vertical section can be generated by using a fixed distance from coast or fixed ocean depth. The user can customize the plot by changing the color-map, the range of the color-bar, the type of the plot (linearly interpolated color, simple contours, filled contours) and download the current view as a simple image or as Keyhole Markup Language (KML) file for visualization in applications such as Google Earth. The data products can also be accessed as NetCDF files and through OPeNDAP. Third-party layers from a web map service can also be integrated. OceanBrowser is used in the frame of the SeaDataNet project (http://gher-diva.phys.ulg.ac.be/web-vis/) and EMODNET Chemistry (http://oceanbrowser.net/emodnet/) to distribute gridded data sets interpolated from in situ observation using DIVA (Data-Interpolating Variational Analysis).

  16. Organizācijas pievilcīguma saistība ar personības iezīmēm

    OpenAIRE

    Dzenis, Jānis

    2006-01-01

    Pētījuma mērķis ir noteikt ideālas organizācijas pievilcīgumu raksturojošās pazīmes un izpētīt saistību starp ideālas organizācijas pievilcīgumu raksturojošām pazīmēm un potenciālo darbinieku personības iezīmēm. Pētījums tika veikts divas daļās. Pirmajā daļā tika veiktas divas fokusa grupas, kurās kopā piedalījās 16 dalībnieki (n1 – 8; n2 – 8). Otrajā pētījuma daļā piedalījās 2.-3. kursa studenti (N=54; M=20,9). Šī pētījuma ietvaros tika apskatītas četras organizāciju raksturojošās pazīmes – ...

  17. New Mediterranean Biodiversity Records (July 2015

    Directory of Open Access Journals (Sweden)

    K. TSIAMIS

    2015-07-01

    Full Text Available The Collective Article ‘New Mediterranean Biodiversity Records’ of the Mediterranean Marine Science journal offers the means to publish biodiversity records in the Mediterranean Sea. The current article is divided in two parts, for records of native and alien species respectively. The new records of native species include: the neon flying squid Ommastrephes bartramii in Capri Island, Thyrrenian Sea; the bigeye thresher shark Alopias superciliosus in the Adriatic Sea; a juvenile basking shark Cetorhinus maximus caught off Piran (northern Adriatic; the deep-sea Messina rockfish Scorpaenodes arenai in the National Marine Park of Zakynthos (East Ionian Sea, Greece; and the oceanic puffer Lagocephalus lagocephalus in the Adriatic Sea.The new records of alien species include: the red algae Antithamnionella elegans and Palisada maris-rubri, found for the first time in Israel and Greece respectively; the green alga Codium parvulum reported from Turkey (Aegean Sea; the first record of the alien sea urchin Diadema setosum in Greece; the nudibranch Goniobranchus annulatus reported from South-Eastern Aegean Sea (Greece; the opisthobranch Melibe viridis found in Lebanon; the new records of the blue spotted cornetfish Fistularia commersonii in the Alicante coast (Eastern Spain; the alien fish Siganus luridus and Siganus rivulatus in Lipsi Island, Dodecanese (Greece; the first record of Stephanolepis diaspros from the Egadi Islands Marine Protected Area (western Sicily; a northward expansion of the alien pufferfish Torquigener flavimaculosus along the southeastern Aegean coasts of Turkey; and data on the occurrence of the Lessepsian immigrants Alepes djedaba, Lagocephalus sceleratus and Fistularia commersonii in Zakynthos Island (SE Ionian Sea, Greece.

  18. Progress in new-type vaccines against classical swine fever%新型猪瘟疫苗研究进展

    Institute of Scientific and Technical Information of China (English)

    王春花; 孙元; 仇华吉

    2013-01-01

    Classical swine fever (CSF),an acute and highly contagious disease of swine,is caused by classical swine fever virus.CSF is one of the most devastating diseases to the pig industry worldwide and results in serious economic losses.Currently prophylactic vaccination is still an important strategy for the control of CSF.Live attenuated vaccines (such as C-strain) are safe and effective.However,there are significant changes in the clinical features of CSF,displaying concurrent typical and atypical CSF,and simultaneous inapparent and persistent infections.Immunization failure has been reported frequently and it is difficult to distinguish between wild-type infected and vaccinated animals (DIVA).So there is an urgent need to develop more effective and safer DIVA or marker vaccines for the control of CSF.In this review,some of the most recent advances in new-type vaccines against CSF,including DNA vaccines,live virus-vectored vaccines,protein or peptide-based vaccines,gene-deleted vaccines and chimeric pestivirus-based vaccines,are reviewed and discussed.%猪瘟是由猪瘟病毒引起猪的一种急性、热性和高度接触性传染病.该病呈世界性分布,给世界养猪业造成了巨大的经济损失.目前,疫苗接种仍然是防控猪瘟的主要手段.虽然传统的猪瘟弱毒疫苗(如C株)安全有效,但猪瘟的临床表现发生了很大变化,呈现典型猪瘟和非典型猪瘟共存、隐性感染和持续感染并现,免疫失败的现象时有报道,且不能区分野毒感染和免疫接种.因此,研制安全、高效、能区分野毒感染和疫苗免疫动物(DIVA)的新型猪瘟疫苗极为必要.文中就近年来开发的核酸疫苗、病毒活载体疫苗、基于蛋白/肽的疫苗、基因缺失疫苗、嵌合瘟病毒疫苗等新型DIVA猪瘟疫苗作一综述.

  19. How deep can surface signals be traced in the critical zone? Merging biodiversity with biogeochemistry research in a central German Muschelkalk landscape

    Science.gov (United States)

    Küsel, Kirsten; Totsche, Kai; Trumbore, Susan; Lehmann, Robert; Steinhäuser, Christine; Herrmann, Martina

    2016-04-01

    The Earth's Critical Zone (CZ) is a thin living layer connecting atmosphere and geosphere, including aquifers. Humans live in the CZ and benefit from the vital supporting services it provides. However, the CZ is increasingly impacted by human activities including land and resource use, pollution and climate change. Recent interest in uniting the many disciplines studying this complex domain has initiated an international network of research infrastructure platforms that allow access to the CZ in a range of geologic settings. In this paper a new such infrastructure platform associated with the Collaborative Research Center AquaDiva is described, that uniquely seeks to combine CZ research with detailed investigation of the functional biodiversity of the subsurface. Overall, AquaDiva aims to test hypotheses about how water connects surface conditions set by land cover and land management to the biota and biogeochemical functions in the subsurface. With long-term and continuous observations, hypotheses about how seasonal variations and extreme events at the surface impact subsurface processes, community structure and function, are tested. AquaDiva has established the Hainich Critical Zone Exploratory (CZE) in central Germany in an alkaline geological setting of German Triassic Muschelkalk formations. The Hainich CZE includes specialized monitoring wells to access the vadose zone and two main groundwater complexes in limestone and marlstone parent materials along a ~6 km transect spanning forest, pasture and agricultural land uses. Initial results demonstrate fundamental differences in the biota and biogeochemistry of the two aquifer complexes that trace back to the land uses in their respective recharge areas. They also show the importance of antecedent conditions on the impact of precipitation events on responses in terms of groundwater dynamics, chemistry and ecology. Thus we find signals of surface land use and events can be detected in the subsurface CZ. Future

  20. Vikipēdija un tās izmantošana studiju procesā

    OpenAIRE

    Kleinšmite, Ilze

    2015-01-01

    Vikipēdija ir daudzvalodu, bezmaksas tiešsaistes enciklopēdija, kura tika izveidota 2001. gadā, un tās popularitāte ir tikai augusi kopš tās pastāvēšanas brīža. Bakalaura darba „Vikipēdija un tās izmantošana studiju procesā” mērķis ir noskaidrot, vai Vikipēdija tiek izmantota pamatstudiju procesā. Bakalaura darbu veido piecas nodaļas. Šajā pētījumā izmantotas divas pētniecības metodes – anketēšana un intervēšana. Darbā raksturota Vikipēdijas vēsture, informācijas sabiedrība un tīmeklis 2.0. P...

  1. Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars.

    Science.gov (United States)

    Widén, F; Everett, H; Blome, S; Fernandez Pinero, J; Uttenthal, A; Cortey, M; von Rosen, T; Tignon, M; Liu, L

    2014-10-01

    Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance.

  2. Comparing cosmic web classifiers using information theory

    CERN Document Server

    Leclercq, Florent; Jasche, Jens; Wandelt, Benjamin

    2016-01-01

    We introduce a decision scheme for optimally choosing a classifier, which segments the cosmic web into different structure types (voids, sheets, filaments, and clusters). Our framework, based on information theory, accounts for the design aims of different classes of possible applications: (i) parameter inference, (ii) model selection, and (iii) prediction of new observations. As an illustration, we use cosmographic maps of web-types in the Sloan Digital Sky Survey to assess the relative performance of the classifiers T-web, DIVA and ORIGAMI for: (i) analyzing the morphology of the cosmic web, (ii) discriminating dark energy models, and (iii) predicting galaxy colors. Our study substantiates a data-supported connection between cosmic web analysis and information theory, and paves the path towards principled design of analysis procedures for the next generation of galaxy surveys. We have made the cosmic web maps, galaxy catalog, and analysis scripts used in this work publicly available.

  3. Comparing cosmic web classifiers using information theory

    Science.gov (United States)

    Leclercq, Florent; Lavaux, Guilhem; Jasche, Jens; Wandelt, Benjamin

    2016-08-01

    We introduce a decision scheme for optimally choosing a classifier, which segments the cosmic web into different structure types (voids, sheets, filaments, and clusters). Our framework, based on information theory, accounts for the design aims of different classes of possible applications: (i) parameter inference, (ii) model selection, and (iii) prediction of new observations. As an illustration, we use cosmographic maps of web-types in the Sloan Digital Sky Survey to assess the relative performance of the classifiers T-WEB, DIVA and ORIGAMI for: (i) analyzing the morphology of the cosmic web, (ii) discriminating dark energy models, and (iii) predicting galaxy colors. Our study substantiates a data-supported connection between cosmic web analysis and information theory, and paves the path towards principled design of analysis procedures for the next generation of galaxy surveys. We have made the cosmic web maps, galaxy catalog, and analysis scripts used in this work publicly available.

  4. Relationship between the sporocarp and pseudosclerotia size of Lentinus velutinus (Lentinaceae-Poriales, validation of a hypothesis suggested by the Guaranis of Misiones, Argentina RELACIÓN ENTRE LAS DIMENSIONES DEL CUERPO FRUCTÍFERO Y EL PSEUDOESCLEROCIO DE LENTINUS VELUTINUS (LENTINACEAE-PORIALES, VALIDACIÓN DE UNA HIPÓTESIS SUGERIDA POR LOS GUARANÍES DE MISIONES, ARGENTINA

    Directory of Open Access Journals (Sweden)

    Héctor A. Keller

    2010-07-01

    Full Text Available <div>A regression analysis that allows to establish relationships between the size of the sporocarp

and the size of the pseudoesclerotia of the mushroom Lentinus velutinus is presented. This
relationship was suggested for informants of guarani communities of Misiones Province,
Argentina, they use the pseudoesclerotia to make bowls of traditional pipes
Se presenta un análisis de regresión que permite establecer relaciones entre el tamaño del
cuerpo fructífero y el tamaño del pseudoesclerocio del hongo Lentinus velutinus. Dicha
relación fue sugerida por integrantes de aldeas guaraníes de Misiones, Argentina, quienes
emplean los pseudoesclerocios como materia prima para confeccionar cuencos de pipas
tradicionales

  • A emergência de cosmovisões relacionais

    Directory of Open Access Journals (Sweden)

    Veronese, Marília Veríssimo

    2006-01-01

    Full Text Available O artigo aborda três conceitos, oriundos de campos diversos como a sociologia, antropologia e psicologia social. São eles: a cultura da partilha, o paradigma da dádiva e o comunitarismo solidário. São inicialmente caracterizados, depois comparados em termos de suas semelhanças e diferenças, numa análise crítica que resulta na emergência do conceito de cosmovisões relacionais. Como uma cosmovisão se traduz em práticas, discute-se o que cada paradigma apresentado veicula, em termos propositivos, para a sociedade, o sujeito e o trabalho

  • EMODnet Thematic Lot n° 4 - Chemistry

    DEFF Research Database (Denmark)

    Beckers, Jean-Marie; Buga, Luminita; Debray, Noelie

    2015-01-01

    Data quality assurance and quality control (QA/QC) is an important issue in oceanographic data management, especially for the creation of multidisciplinary and comprehensive databases which include data from different and/or unknown origin covering long time periods. The data-collection methods...... inconsistent data quality flags and the need for coordination and harmonization of practices. A dedicated workshop was organized to review the different practices and agree on a common methodology for data QA/QC and Diva products generation for EMODnet Chemistry....... will contribute considerably to the validation of large data collections. This report intends to be a reference manual for EMODnet Chemistry data QA/QC and the subsequent product generation. In fact, during the first data validation loop, each region adopted its own protocol and the results showed many...

  • Brand “NIKE”, its signs and arguments: a study of applied semiotic

    Directory of Open Access Journals (Sweden)

    Vinícius dos Santos Souza

    2016-05-01

    Full Text Available This study elected as its research object the logo of the call sporting products company Nike; as regards to the Greek goodness Nice: diva of the victory. Based on Peirce's semiotics, our aim to identify and understand the perceptive and historical foundations responsible for sustaining the said logo as a sign capable of representing the company in their business purposes. We aim to demonstrate that the selection of logo for the company's founders follows a line of argument focused on building of semiotic links - perceptive and historical - between the brand, its products and the behavior of their consumers. The logo implies a dedicated language for the possibility of a communicative link between the product and the subjectivity of its consumer; so that the first former becomes logically part of thoughts and behaviors of the second.

  • EMODnet Thematic Lot n° 4 - Chemistry

    DEFF Research Database (Denmark)

    Beckers, Jean-Marie; Buga, Luminita; Debray, Noelie

    2015-01-01

    Data quality assurance and quality control (QA/QC) is an important issue in oceanographic data management, especially for the creation of multidisciplinary and comprehensive databases which include data from different and/or unknown origin covering long time periods. The data-collection methods i...... inconsistent data quality flags and the need for coordination and harmonization of practices. A dedicated workshop was organized to review the different practices and agree on a common methodology for data QA/QC and Diva products generation for EMODnet Chemistry....... will contribute considerably to the validation of large data collections. This report intends to be a reference manual for EMODnet Chemistry data QA/QC and the subsequent product generation. In fact, during the first data validation loop, each region adopted its own protocol and the results showed many...

  • Análisis espacial y conservación de los anfibios y reptiles del Estado de México

    Directory of Open Access Journals (Sweden)

    Xóchitl Aguilar Miguel

    2009-01-01

    Full Text Available Se calculó la riqueza y diversidad alfa y beta de los anfibios y reptiles del Estado de México, para identificar las regiones de mayor importancia para su estudio y conservación. Se analizaron 10 992 registros. Se calculó la riqueza de especies y los índices de Simpson y Whittaker, mediante diva 5.0. Se digitalizaron las áreas con mayor valor de diversidad alfa, diversidad beta y riqueza de especies, traslapándolas para identificar las de mayor valor para conservación. Se obtuvieron valores altos de diversidad alfa en muchas partes del estado, mientras que la diversidad beta muestra una situación intermedia. Las tres zonas de mayor valor para la conservación se ubican en el centro-sur, suroeste y oriente, por su mayor número de criterios de conservación.

  • Efficacy of ibandronate: a long term confirmation.

    Science.gov (United States)

    Di Munno, Ombretta; Delle Sedie, Andrea

    2010-01-01

    Data deriving from randomized clinical trials, observational studies and meta-analyses, including treatment regimens unlicensed for use in clinical practice, clearly support that 150 mg once-monthly oral and 3 mg quarterly i.v. doses of ibandronate are associated with efficacy, safety and tolerability; notably both these marketed regimens, which largely correspond to ACE ≥10.8 mg, may in addition provide a significant efficacy on non-vertebral and clinical fracture (Fx) efficacy. The MOBILE and the DIVA LTE studies confirmed a sustained efficacy of monthly oral and quarterly i.v. regimens respectively, over 5 years. Furthermore, improved adherence rates with monthly ibandronate, deriving from studies evaluating large prescription databases, promise to enhance fracture protection and decrease the social and economic burden of postmenopausal osteoporosis.

  • Inference and classifications of the Lagrangian dark matter sheet in the SDSS

    CERN Document Server

    Leclercq, Florent; Lavaux, Guilhem; Wandelt, Benjamin

    2016-01-01

    Whereas previous studies have demonstrated that the shape of the cosmic web can be described by studying the Lagrangian displacement field, state-of-the-art analyses have been limited to cosmological simulations. This letter reports on the possibility to perform a Lagrangian description of cosmic web environments in real data from large-scale structure surveys. Building upon recent Bayesian large-scale inference of initial conditions, we present an analysis of the Lagrangian dark matter sheet in the nearby universe as probed by the Sloan Digital Sky Survey. In particular, we consider its stretchings and foldings and we dissect cosmic structures into four distinct components (voids, sheets, filaments, and clusters), using the Lagrangian classifiers DIVA and ORIGAMI. As a result, identified structures explicitly carry physical information about their formation history. The present study carries potential for profound implications in the analysis of the cosmological large-scale structure, as it opens the way for...

  • Georadar - high resolution geophysical electromagnetic device

    Directory of Open Access Journals (Sweden)

    Janez Stern

    1995-12-01

    Full Text Available Georadar is a high resolution geophysical electromagnetic device that was developed in the first part of the 1980's. In Slovenia it was first tested in 1991 on several objects of economicgeological, geotechnical and hydrogeologic nature.Here its usefulness in karst studied is presented. The first part of the paper deals with description of measurement procedure and methodological bases, and the second part with experience and results of case histories. Shown are radargrams from ornamental stone quarry Hotavlje, calcite mine Stahovica, Golobja jama karstcave near Divača and from highway construction site Razdrto-Čebulovica. All measurements were performed with the georadar instrument Pulse EKKO IV with a lOOMHz antenna according to the method of reflection profiling.

  • Nonlinear elliptic systems with exponential nonlinearities

    Directory of Open Access Journals (Sweden)

    Said El Manouni

    2002-12-01

    Full Text Available In this paper we investigate the existence of solutions for {gather*} -mathop{m div}( a(| abla u | ^N| abla u |^{N-2}u = f(x,u,v quad mbox{in } Omega -mathop{m div}(a(| abla v| ^N| abla v |^{N-2}v = g(x,u,v quad mbox{in } Omega u(x = v(x = 0 quad mbox{on }partial Omega. end{gather*} Where $Omega$ is a bounded domain in ${mathbb{R}}^N$, $Ngeq 2$, $f$ and $g$ are nonlinearities having an exponential growth on $Omega$ and $a$ is a continuous function satisfying some conditions which ensure the existence of solutions.

  • VEP un stereoredze

    OpenAIRE

    2006-01-01

    Darba mērķis. Ar VEP iekārtu noteikt stereo stimula ietekmi VEP atbildēs, apstrādājot datus ar Furjē analīzi. Darba uzdevumi. •Izveidot iekārtu un stimulus stereo VEP atbildes iegūšanai; •Novērtēt Furjē analīzes pielietošanas iespējas stereo VEP rezultātu analīzē; •Iegūt monokulāru, binokulāru un stereo VEP; •Savstarpēji salīdzināt divas dažādas metodes stereo VEP iegūšanai. Subjekti. Pētījumā piedalījās 4 subjekti vecumā no 24 līdz 34 gadiem. Visiem netraucēta bin...

  • Vaccination with recombinant adenoviruses expressing the peste des petits ruminants virus F or H proteins overcomes viral immunosuppression and induces protective immunity against PPRV challenge in sheep.

    Science.gov (United States)

    Rojas, José M; Moreno, Héctor; Valcárcel, Félix; Peña, Lourdes; Sevilla, Noemí; Martín, Verónica

    2014-01-01

    Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants caused by the Morbillivirus peste des petits ruminants virus (PPRV). Two recombinant replication-defective human adenoviruses serotype 5 (Ad5) expressing either the highly immunogenic fusion protein (F) or hemagglutinin protein (H) from PPRV were used to vaccinate sheep by intramuscular inoculation. Both recombinant adenovirus vaccines elicited PPRV-specific B- and T-cell responses. Thus, neutralizing antibodies were detected in sera from immunized sheep. In addition, we detected a significant antigen specific T-cell response in vaccinated sheep against two different PPRV strains, indicating that the vaccine induced heterologous T cell responses. Importantly, no clinical signs and undetectable virus shedding were observed after virulent PPRV challenge in vaccinated sheep. These vaccines also overcame the T cell immunosuppression induced by PPRV in control animals. The results indicate that these adenovirus constructs could be a promising alternative to current vaccine strategies for the development of PPRV DIVA vaccines.

  • Long-life tomato cultivars growing under the hydroponic Nutrient Film Technique

    Directory of Open Access Journals (Sweden)

    Gualberto Ronan

    2002-01-01

    Full Text Available Farmers are interested in hydroponic tomato cultivation because of the high demand of high quality products by the consumers. Three trials here carried out under greenhouse conditions in Marília, SP, Brazil, using the hydroponic nutrient film technique, in order to evaluate yield and quality of long-life salad tomato (Lycopersicon esculentum varieties, during 1998 and 1999. Four varieties (Carmen, Diva, Graziela, and Vita were tested using a randomized block design, with six replications. The variety x crop season interaction effect was significant, meaning that varieties have distinct performances during the different cropping seasons. The Vita variety presented commercial valid yield, with mean fruit weights higher than the other varieties.

  • "Divina": consagración cultural y usos de lo sagrado en la actriz mexicana María Félix (1914-2002

    Directory of Open Access Journals (Sweden)

    Carolina Benavente Morales

    2010-01-01

    Full Text Available Este estudio gira en torno a la noción de consagración cultural de Pierre Bourdieu enfocado a la actriz mexicana María Félix. Por un lado, se le aborda como una diva consagrada por las clases medias en ascenso en el contexto de la "época dorada" del cine mexicano, y, por el otro, como una mujer que agencia su propia consagración mediante una performance de vedette. Al vincular la persona a dinámicas y sectores sociales concretos, se observa la existencia de múltiples y divergentes libretos sobre la historia del siglo XX mexicano, y se intenta situar en relación con ellos la performance de la actriz.

  • 三位歌剧女主角闪亮纽约舞台

    Institute of Scientific and Technical Information of China (English)

    谢朝宗

    2015-01-01

    <正>"Diva"(歌剧中的女主演)是歌剧里最为迷人的字眼之一,无论在舞台上还是舞台之外,她们都能牵动歌剧迷们最炽热的情绪。纽约本季的歌剧在女演员这方面相当有看头,到目前为止已经有三部演出的歌剧女主角,可构得上令人崇拜的Diva标准了。首先,是安娜·涅特里布科(Anna Netrebko)在《麦克白》(Macbeth)里的演出。这出威尔第早期向中期过渡的重要作品,虽

  • Effect of Ultrasound-Guided Placement of Difficult-to-Place Peripheral Venous Catheters

    DEFF Research Database (Denmark)

    Partovi-Deilami, Kohyar; Nielsen, Jesper; Moller, Ann M

    2016-01-01

    ) with ultrasound. Procedure time was reduced from 20 to 10 minutes, discomfort was unchanged, and the median number of skin punctures decreased from 3 to 2. The incidence of central venous catheter placement dropped from 34% to 7%. Implementation of a training program and a mobile service in which nurse...... operated by nurse anesthetists for these patients. This prospective observational study with a pre/post design focused on inpatients with DIVA referred for PVC placement, a service provided by nurse anesthetists in most Scandinavian hospitals. The rate of success, procedure time, number of skin punctures......, discomfort, catheter size, location, and incidence of central venous catheter placement are reported before and after implementation of a training program and a mobile service using ultrasound to place difficult-to-place PVCs. The success rate increased from 0% (0 of 33 patients) to 83% (58 of 70 patients...

  • Para uma abordagem institucionalista do voluntariado – o caso do voluntário da Cruz Vermelha portuguesa

    Directory of Open Access Journals (Sweden)

    Armando Fernandes

    2016-03-01

    Full Text Available Este artigo discute o voluntariado e a acção voluntária sob uma perspectiva institucionalista. Nessa perspectiva, o voluntariado é compreendido como o resultado de instituições próprias, nomeadamente, a geração de contextos socioeconómicos estimuladores da expressão da dádiva (essência da acção voluntária mas também de contextos emergentes (momentos de crise não satisfeitos pela acção combinada dos mercados e do Estado. Para esforços de ilustração, recorremos a dois exemplos analisados em detalhe – os exemplos do caso português e o exemplo da Cruz Vermelha.

    1. Synthesis and Antimicrobial activity 2-chloro-6-methylquinoline Hydrazone derivatives

      Directory of Open Access Journals (Sweden)

      Rajiv Kumar

      2009-12-01

      Full Text Available <div>A series of 2-chloro-6-methylquinoline hydrazones (3a-o were synthesized by the condensation of substituted acyl

    hydrazines, semicarbazide, thiosemicarbazide and INH with 2-chloro-3-formyl-6-methylquinoline in absolute alcohol.
    The structures of compounds were established using spectral data and elemental analysis. All the compounds were
    evaluated for their antibacterial activity against Escherichia coli (NCTC, 10418, Staphylococcus aureus (NCTC, 65710
    and Pseudomonas aeruginosa (NCTC, 10662. Compounds were also tested for antifungal activity aganist Aspergillus
    niger (MTCC, 281, Aspergillus flavus (MTCC, 277, Monascus purpureus (MTCC, 369 and Penicillium citrinum
    (NCIM, 768 by cup-plate method.

  • Historical biogeographic analysis of the family Fanniidae (Díptera: Calyptratae, with special reference to the austral species of the genus Fannia (Diptera: Fanniidae using dispersal-vicariance analysis Análisis biogeográfico histórico de la familia Fanniidae (Diptera: Calyptratae, con referencia especial a las especies australes del genero Fannia (Diptera: Fanniidae usando análisis de dipersion-vicarianza

    Directory of Open Access Journals (Sweden)

    M. CECILIA DOMÍNGUEZ

    2011-03-01

    Full Text Available The purpose of this study was to achieve a hypothesis explaining the biogeographical history of the family Fanniidae, especially that of the species from Patagonia, the Neotropics, Australia, and New Zealand. We used "dispersal-vicariance analysis" (DIVA, an event-based parsimony method, to analyze the most parsimonious phylogenetic hypothesis for the family, obtained by Domínguez & Roig-Juñent (2008. The analysis resulted in 32800 alternative equally optimal reconstructions that indicate that the ancestor of the Fanniidae was widely distributed across different regions of the world, which along with the subsequent separation of two clades that correspond to the Laurasic and Gondwanan Landmasses allow the proposal of an older age than in previous hypothesis (Late Jurassic or early Cretaceous times instead of upper Cretaceous and a Pangeic origin for the Fanniidae. The northern hemisphere species of Fanniidae included in this study highlight the difficulty that arises when analysing with DIVA a tree with a large amount of paralogy or redundant distributions, as illustrated here with several examples. The southern hemisphere species of Fanniidae indicate a clear pattern of vicariance and dispersal consistent with the rupture of Gondwana.El propósito de este estudio fue el de obtener una hipótesis que explique la historia biogeográfica de la familia Fanniidae, especialmente la de las especies de las regiones Patagónica, Neotropical, Australiana y Neozelandesa. Se utilizó el método de "dispersión y vicarianza" (DIVA, el cual es un método de parsimonia basado en eventos para analizar el árbol filogenético más parsimonioso obtenido por Domínguez & Roig-Juñent (2008. El análisis resultó en 32800 reconstrucciones alternativas igualmente óptimas que indican que el ancestro de Fanniidae estaba ampliamente distribuido en distintas regiones del mundo, lo cual junto con la subsiguiente separación de dos clados que corresponderían a los

  • Effects of vaccination against paratuberculosis on tuberculosis in goats: diagnostic interferences and cross-protection

    Directory of Open Access Journals (Sweden)

    Pérez de Val Bernat

    2012-10-01

    Full Text Available Abstract Background Most countries carrying out campaigns of bovine tuberculosis (TB eradication impose a ban on the use of mycobacterial vaccines in cattle. However, vaccination against paratuberculosis (PTB in goats is often allowed even when its effect on TB diagnosis has not been fully evaluated. To address this issue, goat kids previously vaccinated against PTB were experimentally infected with TB. Results Evaluation of interferon-γ (IFN-γ secretion induced by avian and bovine tuberculins (PPD showed a predominant avian PPD-biased response in the vaccinated group from week 4 post-vaccination onward. Although 60% of the animals were bovine reactors at week 14, avian PPD-biased responses returned at week 16. After challenge with M. caprae, the IFN-γ responses radically changed to show predominant bovine PPD-biased responses from week 18 onward. In addition, cross-reactions with bovine PPD that had been observed in the vaccinated group at week 14 were reduced when using the M. tuberculosis complex-specific antigens ESAT-6/CFP-10 and Rv3615c as new DIVA (differentiation of infected and vaccinated animals reagents, which further maintained sensitivity post-challenge. Ninety percent of the animals reacted positively to the tuberculin cervical comparative intradermal test performed at 12 weeks post-infection. Furthermore, post-mortem analysis showed reductions in tuberculous lesions and bacterial burden in some vaccinated animals, particularly expressed in terms of the degree of extrapulmonary dissemination of TB infection. Conclusions Our results suggest a degree of interference of PTB vaccination with current TB diagnostics that can be fully mitigated when using new DIVA reagents. A partial protective effect associated with vaccination was also observed in some vaccinated animals.

  • Auditory-motor interactions in pediatric motor speech disorders: neurocomputational modeling of disordered development.

    Science.gov (United States)

    Terband, H; Maassen, B; Guenther, F H; Brumberg, J

    2014-01-01

    Differentiating the symptom complex due to phonological-level disorders, speech delay and pediatric motor speech disorders is a controversial issue in the field of pediatric speech and language pathology. The present study investigated the developmental interaction between neurological deficits in auditory and motor processes using computational modeling with the DIVA model. In a series of computer simulations, we investigated the effect of a motor processing deficit alone (MPD), and the effect of a motor processing deficit in combination with an auditory processing deficit (MPD+APD) on the trajectory and endpoint of speech motor development in the DIVA model. Simulation results showed that a motor programming deficit predominantly leads to deterioration on the phonological level (phonemic mappings) when auditory self-monitoring is intact, and on the systemic level (systemic mapping) if auditory self-monitoring is impaired. These findings suggest a close relation between quality of auditory self-monitoring and the involvement of phonological vs. motor processes in children with pediatric motor speech disorders. It is suggested that MPD+APD might be involved in typically apraxic speech output disorders and MPD in pediatric motor speech disorders that also have a phonological component. Possibilities to verify these hypotheses using empirical data collected from human subjects are discussed. The reader will be able to: (1) identify the difficulties in studying disordered speech motor development; (2) describe the differences in speech motor characteristics between SSD and subtype CAS; (3) describe the different types of learning that occur in the sensory-motor system during babbling and early speech acquisition; (4) identify the neural control subsystems involved in speech production; (5) describe the potential role of auditory self-monitoring in developmental speech disorders. Copyright © 2014 Elsevier Inc. All rights reserved.

  • Preliminary assessment of the potential for mangrove oyster cultivation in Piraquê-açu river estuary (Aracruz, ES

    Directory of Open Access Journals (Sweden)

    Luciana Alvarenga

    2006-01-01

    Full Text Available At Piraquê-açu river estuary, Aracruz, ES, the technical viability of Crassostrea rhizophorae cultivation was determined through monthly measures in shell length and weight. Seeds of C. rhizophorae were put in cages and suspended in rafts. Increase in height and weight (flesh and dry of the oysters was measured. During ten months (July/98 to May/99, oyster shell reached an average of 37.6 mm in shell height and 3.0 g in flesh weight (the whole animal. High mortality rates were registered and could be related to the high salinity water and to high predation observed, especially by flatworms Stylochophana divae and snails Cymatium parthenopeum, as well as fouling organisms such as barnacles, Serpulidae polychaetes and seed of the same species.No estuário do Rio Piraquê-açu, município de Aracruz, ES, foi avaliada a viabilidade técnica da implantação do cultivo de ostras Crassostrea rhizophorae em lanternas suspensas numa balsa de cultivo, através da estimativa de crescimento e ganho de peso fresco e seco das ostras. Verificou-se que, durante os dez meses de cultivo (junho/98 a maio/99, as ostras atingiram, em média, 37,6 mm de altura e 3,0 g no peso total. Foi registrada uma alta taxa de mortalidade, que poderia ser atribuída aos altos valores de salinidade na água e também aos predadores observados como planárias Stylochoplana divae e gastrópodos Cymatium parthenopeum, além de incrustantes como cracas, poliquetos Serpulidae e principalmente sementes da própria ostra.

  • Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.

    Science.gov (United States)

    Halbherr, Stefan J; Brostoff, Terza; Tippenhauer, Merve; Locher, Samira; Berger Rentsch, Marianne; Zimmer, Gert

    2013-01-01

    Highly pathogenic avian influenza viruses (HPAIV) of subtype H5N1 not only cause a devastating disease in domestic chickens and turkeys but also pose a continuous threat to public health. In some countries, H5N1 viruses continue to circulate and evolve into new clades and subclades. The rapid evolution of these viruses represents a problem for virus diagnosis and control. In this work, recombinant vesicular stomatitis virus (VSV) vectors expressing HA of subtype H5 were generated. To comply with biosafety issues the G gene was deleted from the VSV genome. The resulting vaccine vector VSV*ΔG(HA) was propagated on helper cells providing the VSV G protein in trans. Vaccination of chickens with a single intramuscular dose of 2×10⁸ infectious replicon particles without adjuvant conferred complete protection from lethal H5N1 infection. Subsequent application of the same vaccine strongly boosted the humoral immune response and completely prevented shedding of challenge virus and transmission to sentinel birds. The vaccine allowed serological differentiation of infected from vaccinated animals (DIVA) by employing a commercially available ELISA. Immunized chickens produced antibodies with neutralizing activity against multiple H5 viruses representing clades 1, 2.2, 2.5, and low-pathogenic avian influenza viruses (classical clade). Studies using chimeric H1/H5 hemagglutinins showed that the neutralizing activity was predominantly directed against the globular head domain. In summary, these results suggest that VSV replicon particles are safe and potent DIVA vaccines that may help to control avian influenza viruses in domestic poultry.

  • E as plantations viraram fumaça: nobreza principal da terra, Antigo Regime e escravidão mercantil

    Directory of Open Access Journals (Sweden)

    João FRAGOSO

    2015-12-01

    Full Text Available Resumo O artigo parte do pressuposto de que as famílias dos conquistadores e dos primeiros povoadores do Rio de Janeiro quinhentista, nos séculos seguintes, formaram um grupo social capaz de elaborar os seus mecanismos de reprodução social no tempo: no caso, trata-se da nobreza principal da terra. Os eixos desses mecanismos não foram tanto o controle da terra e da mão de obra - através, por exemplo, do sistema de vínculos (morgadios e capelas ou por meio do mercado -, mas mediante os valores do Antigo Regime católico. Entre tais valores temos: o de prestígio social (fidalguia de pergaminhos e a costumeira dada pela conquista e de dádivas (troca pessoal desigual. Entre os resultados desse processo temos a fabricação de uma sociedade hierarquizada e pautada na ideia de superioridade social como sinônimo de viver do trabalho alheio. Nesse contexto a prática dádiva surge como movimento fundamental, pois era capaz, ao mesmo tempo, de reafirmar superioridade social e da promoção social, a exemplo da alforria. A Nobreza principal da terra, no Rio de Janeiro, sucumbiu no século XVIII; porém, a sociedade e o seu ideário sobreviveram, inclusive entre os grupos ditos subalternos. Estas hipóteses foram construídas a partir da empiria do Rio de Janeiro dos séculos XVII e XVIII, mas procuram contribuir para o entendimento da América lusa baseada no Antigo Regime luso e na escravidão mercantil.

  • Fast thermal simulations and temperature optimization for hyperthermia treatment planning, including realistic 3D vessel networks.

    Science.gov (United States)

    Kok, H P; van den Berg, C A T; Bel, A; Crezee, J

    2013-10-01

    Accurate thermal simulations in hyperthermia treatment planning require discrete modeling of large blood vessels. The very long computation time of the finite difference based DIscrete VAsculature model (DIVA) developed for this purpose is impractical for clinical applications. In this work, a fast steady-state thermal solver was developed for simulations with realistic 3D vessel networks. Additionally, an efficient temperature-based optimization method including the thermal effect of discrete vasculature was developed. The steady-state energy balance for vasculature and tissue was described by a linear system, which was solved with an iterative method on the graphical processing unit. Temperature calculations during optimization were performed by superposition of several precomputed temperature distributions, calculated with the developed thermal solver. The thermal solver and optimization were applied to a human anatomy, with the prostate being the target region, heated with the eight waveguide 70 MHz AMC-8 system. Realistic 3D pelvic vasculature was obtained from angiography. Both the arterial and venous vessel networks consisted of 174 segments and 93 endpoints with a diameter of 1.2 mm. Calculation of the steady-state temperature distribution lasted about 3.3 h with the original DIVA model, while the newly developed method took only ≈ 1-1.5 min. Temperature-based optimization with and without taking the vasculature into account showed differences in optimized waveguide power of more than a factor 2 and optimized tumor T90 differed up to ≈ 0.5°C. This shows the necessity to take discrete vasculature into account during optimization. A very fast method was developed for thermal simulations with realistic 3D vessel networks. The short simulation time allows online calculations and makes temperature optimization with realistic vasculature feasible, which is an important step forward in hyperthermia treatment planning.

  • Recent long-distance dispersal overshadows ancient biogeographical patterns in a pantropical angiosperm family (Simaroubaceae, Sapindales).

    Science.gov (United States)

    Clayton, Joshua W; Soltis, Pamela S; Soltis, Douglas E

    2009-08-01

    Detailed biogeographic studies of pantropical clades are still relatively few, and those conducted to date typically use parsimony or event-based methods to reconstruct ancestral areas. In this study, a recently developed likelihood method for reconstructing ancestral areas (the dispersal-extinction cladogenesis [DEC] model) is applied to the angiosperm family Simaroubaceae, a geographically widespread and ecologically diverse clade of pantropical and temperate trees and shrubs. To estimate divergence dates in the family, Bayesian uncorrelated rates analyses and robust fossil calibrations are applied to the well-sampled and strongly supported phylogeny. For biogeographic analyses, the effects of parameter configurations in the DEC model are assessed for different possible ancestral ranges, and the likelihood method is compared with dispersal-vicariance analysis (DIVA). Regardless of the parameters used, likelihood analyses show a common pattern of multiple recent range shifts that overshadow reconstruction of events deeper in the family's history. DIVA produced results similar to the DEC model when ancestral ranges were restricted to two areas, but some improbable ancestral ranges were also observed. Simaroubaceae exhibit an early history of range expansion between major continental areas in the Northern Hemisphere, but reconstruction of ancestral areas for lineages diverging in the early Tertiary are sensitive to the parameters of the model used. A North American origin is suggested for the family, with migration via Beringia by ancestral taxa. In contrast to traditional views, long-distance dispersal events are common, particularly in the Late Oligocene and later. Notable dispersals are inferred to have occurred across the Atlantic Ocean in both directions, as well as between Africa and Asia, and around the Indian Ocean basin and Pacific islands.

  • Tracking character evolution and biogeographic history through time in Cornaceae-Does choice of methods matter?%追溯山茱萸科植物的性状进化和生物地理学历史——方法选择的影响?

    Institute of Scientific and Technical Information of China (English)

    向秋云; David T. THOMAS

    2008-01-01

    This study compares results on reconstructing the ancestral state of characters and ancestral areas of distribution in Cornaceae to gain insights into the impact of using different analytical methods. Ancestral character state reconstructions were compared among three methods (parsimony, maximum likelihood, and stochastic character mapping) using MESQUITE and a full Bayesian method in BAYESTRAITS and inferences of ancestral area distribution were compared between the parsimony-based dispersal-vicariance analysis (DIVA) and a newly developed maximum likelihood (ML) method. Results indicated that among the six inflorescence and fruit characters examined, "perfect" binary characters (no homoplasy, no polymorphism within terminals, and no missing data) are little affected by choice of method, while homoplasious characters and missing data are sensitive to methods used. Ancestral areas at deep nodes of the phylogeny are substantially different between DIVA and ML and strikingly different between analyses including and excluding fossils at three deepest nodes. These results, while raising caution in making conclusions on trait evolution and historical biogeography using conventional methods, demonstrate a limitation in our current understanding of character evolution and biogeography. The biogeographic history favored by the ML analyses including fossils suggested the origin and early radiation of Cornus likely occurred in the late Cretaceous and earliest Tertiary in Europe and intercontinental disjunctions in three lineages involved movements across the North Atlantic Land Bridge (BLB) in the early and mid Tertiary. This result is congruent with the role of NALB for post-Eocene migration and in connecting tropical floras in North America and Africa, and in eastern Asia and South America. However, alternative hypotheses with an origin in eastern Asia and early Trans-Beringia migrations of the genus cannot be ruled out.

  • Vaccination with recombinant RNA replicon particles protects chickens from H5N1 highly pathogenic avian influenza virus.

    Directory of Open Access Journals (Sweden)

    Stefan J Halbherr

    Full Text Available Highly pathogenic avian influenza viruses (HPAIV of subtype H5N1 not only cause a devastating disease in domestic chickens and turkeys but also pose a continuous threat to public health. In some countries, H5N1 viruses continue to circulate and evolve into new clades and subclades. The rapid evolution of these viruses represents a problem for virus diagnosis and control. In this work, recombinant vesicular stomatitis virus (VSV vectors expressing HA of subtype H5 were generated. To comply with biosafety issues the G gene was deleted from the VSV genome. The resulting vaccine vector VSV*ΔG(HA was propagated on helper cells providing the VSV G protein in trans. Vaccination of chickens with a single intramuscular dose of 2×10⁸ infectious replicon particles without adjuvant conferred complete protection from lethal H5N1 infection. Subsequent application of the same vaccine strongly boosted the humoral immune response and completely prevented shedding of challenge virus and transmission to sentinel birds. The vaccine allowed serological differentiation of infected from vaccinated animals (DIVA by employing a commercially available ELISA. Immunized chickens produced antibodies with neutralizing activity against multiple H5 viruses representing clades 1, 2.2, 2.5, and low-pathogenic avian influenza viruses (classical clade. Studies using chimeric H1/H5 hemagglutinins showed that the neutralizing activity was predominantly directed against the globular head domain. In summary, these results suggest that VSV replicon particles are safe and potent DIVA vaccines that may help to control avian influenza viruses in domestic poultry.

  • Evaluation of a Salmonella Strain Lacking the Secondary Messenger C-di-GMP and RpoS as a Live Oral Vaccine

    Science.gov (United States)

    García, Begoña; Gil, Carmen; García-Ona, Enrique; Burgui, Saioa; Casares, Noelia; Hervás-Stubbs, Sandra; Lasarte, Juan José; Lasa, Iñigo

    2016-01-01

    Salmonellosis is one of the most important bacterial zoonotic diseases transmitted through the consumption of contaminated food, with chicken and pig related products being key reservoirs of infection. Although numerous studies on animal vaccination have been performed in order to reduce Salmonella prevalence, there is still a need for an ideal vaccine. Here, with the aim of constructing a novel live attenuated Salmonella vaccine candidate, we firstly analyzed the impact of the absence of cyclic-di-GMP (c-di-GMP) in Salmonella virulence. C-di-GMP is an intracellular second messenger that controls a wide range of bacterial processes, including biofilm formation and synthesis of virulence factors, and also modulates the host innate immune response. Our results showed that a Salmonella multiple mutant in the twelve genes encoding diguanylate cyclase proteins that, as a consequence, cannot synthesize c-di-GMP, presents a moderate attenuation in a systemic murine infection model. An additional mutation of the rpoS gene resulted in a synergic attenuating effect that led to a highly attenuated strain, referred to as ΔXIII, immunogenic enough to protect mice against a lethal oral challenge of a S. Typhimurium virulent strain. ΔXIII immunogenicity relied on activation of both antibody and cell mediated immune responses characterized by the production of opsonizing antibodies and the induction of significant levels of IFN-γ, TNF-α, IL-2, IL-17 and IL-10. ΔXIII was unable to form a biofilm and did not survive under desiccation conditions, indicating that it could be easily eliminated from the environment. Moreover, ΔXIII shows DIVA features that allow differentiation of infected and vaccinated animals. Altogether, these results show ΔXIII as a safe and effective live DIVA vaccine. PMID:27537839

  • O CHOURIÇO NO SERIDÓ: TRANSFORMAÇÃO DO SANGUE EM DOCE

    Directory of Open Access Journals (Sweden)

    Maria Isabel Dantas

    2007-12-01

    Full Text Available A alimentação reflete a diversidade das culturas e o modo de pertencimento a um grupo social. A cozinha expressa um saber complexo em que estão envolvidos práticas, tabus, gostos, prazeres, receitas, exposição pública e distribuição de alimentos, riqueza, organização social e poder. Na região do Seridó, no Rio Grande do Norte, o consumo de alimentos está ligado a espaços, tempos, práticas, situações e comportamentos coletivamente vividos e imaginados pela população. Este artigo apresenta dados etnográficos da festa da matança do porco e do chouriço nessa região. Insistiremos sobre as lógicas simbólicas e sociais que explicam a transformação do sangue, elemento natural por excelência, de alimento perigoso num doce que pode ser consumido com poucas restrições. A festa é um acontecimento extraordinário que simboliza a reinvenção de uma tradição, em que se observa um complexo sistema simbólico e onde se revela uma organização social em torno da família estendida. Apesar da carne de porco e o chouriço serem alimentos proibidos para algumas categorias de pessoas, possuem uma força simbólica capaz produzir momentos festivos e de se constituírem em elementos de trocas. Eles podem ser definidos como comidas-dádivas. Palavras-chaves: alimentação, simbolismo do sangue, festa e dádiva.

  • Evaluation of an Erns-based enzyme-linked immunosorbent assay to distinguish Classical swine fever virus-infected pigs from pigs vaccinated with CP7_E2alf.

    Science.gov (United States)

    Pannhorst, Katrin; Fröhlich, Andreas; Staubach, Christoph; Meyer, Denise; Blome, Sandra; Becher, Paul

    2015-07-01

    Infections with Classical swine fever virus (CSFV) are a major economic threat to pig production. To combat CSF outbreaks and to maintain trade, new marker vaccines were developed that allow differentiation of infected from vaccinated animals (DIVA principle). The chimeric pestivirus CP7_E2alf was shown to be safe and efficacious. Its DIVA strategy is based on the detection of CSFV E(rns)-specific antibodies that are only developed on infection. However, for the new marker vaccine to be considered a valuable control tool, a validated discriminatory assay is needed. One promising candidate is the already commercially available enzyme-linked immunosorbent assay, PrioCHECK CSFV E(rns) ELISA (Prionics BV, Lelystad, The Netherlands). Four laboratories of different European Union member states tested 530 serum samples and country-specific field sera from domestic pigs and wild boar. The ELISA displayed a good robustness. However, based on its reproducibility and repeatability, ranges rather than single values for diagnostic sensitivity and specificity were defined. The ELISA displayed a sensitivity of 90-98% with sera from CSFV-infected domestic pigs. A specificity of 89-96% was calculated with sera from domestic pigs vaccinated once with CP7_E2alf. The ELISA detected CSFV infections in vaccinated domestic pigs with a sensitivity of 82-94%. The sensitivity was lower with sera taken ≤21 days post-challenge indicating that the stage of CSFV infection had a considerable influence on testing. Taken together, the PrioCHECK CSFV E(rns) ELISA can be used for detection of CSFV infections in CP7_E2alf-vaccinated and nonvaccinated domestic pig populations, but should only be applied on a herd basis by testing a defined number of animals.

  • Neural representations and mechanisms for the performance of simple speech sequences.

    Science.gov (United States)

    Bohland, Jason W; Bullock, Daniel; Guenther, Frank H

    2010-07-01

    Speakers plan the phonological content of their utterances before their release as speech motor acts. Using a finite alphabet of learned phonemes and a relatively small number of syllable structures, speakers are able to rapidly plan and produce arbitrary syllable sequences that fall within the rules of their language. The class of computational models of sequence planning and performance termed competitive queuing models have followed K. S. Lashley [The problem of serial order in behavior. In L. A. Jeffress (Ed.), Cerebral mechanisms in behavior (pp. 112-136). New York: Wiley, 1951] in assuming that inherently parallel neural representations underlie serial action, and this idea is increasingly supported by experimental evidence. In this article, we developed a neural model that extends the existing DIVA model of speech production in two complementary ways. The new model includes paired structure and content subsystems [cf. MacNeilage, P. F. The frame/content theory of evolution of speech production. Behavioral and Brain Sciences, 21, 499-511, 1998 ] that provide parallel representations of a forthcoming speech plan as well as mechanisms for interfacing these phonological planning representations with learned sensorimotor programs to enable stepping through multisyllabic speech plans. On the basis of previous reports, the model's components are hypothesized to be localized to specific cortical and subcortical structures, including the left inferior frontal sulcus, the medial premotor cortex, the basal ganglia, and the thalamus. The new model, called gradient order DIVA, thus fills a void in current speech research by providing formal mechanistic hypotheses about both phonological and phonetic processes that are grounded by neuroanatomy and physiology. This framework also generates predictions that can be tested in future neuroimaging and clinical case studies.

  • First assessment of classical swine fever marker vaccine candidate CP7_E2alf for oral immunization of wild boar under field conditions.

    Science.gov (United States)

    Feliziani, Francesco; Blome, Sandra; Petrini, Stefano; Giammarioli, Monica; Iscaro, Carmen; Severi, Giulio; Convito, Luca; Pietschmann, Jana; Beer, Martin; De Mia, Gian Mario

    2014-04-11

    Oral vaccination against classical swine fever (CSF) is a potent tool to control disease outbreaks in wild boar. So far, vaccination campaigns have been carried out using live attenuated vaccines that do not allow serological differentiation of infected from vaccinated animals (DIVA). Although this drawback is acceptable for wild boar, the use of marker vaccines would facilitate studies on disease and vaccination dynamics. Recently, the CSF marker vaccine candidate CP7_E2alf was assessed for oral immunization under laboratory conditions. Promising results prompted efforts to study the vaccine candidate under field conditions and in bait formulation. In this context, two oral vaccination campaigns were carried out with CP7_E2alf bait vaccines in two areas called 'faunistic-hunting farms' in the region of Umbria, Italy. One campaign was conducted using single vaccination, the second with the routinely employed double vaccination strategy. Both campaigns were carried out before concerted hunting actions were performed. Bait uptake, vaccine virus detection and antibody responses were assessed along with inspections upon gutting. As a comparator, seven wild boar were hand-fed with baits under laboratory conditions. In the field, bait uptake ranged from 63.7% to 98.7%, whereas antibody prevalence reached only 33.3-35.1%. The marker serology showed a strong influence of sample quality on the test outcome with a total of 85% of samples being classified correctly. Vaccine virus was not detectable. Under hand feeding conditions, six out of seven wild boar took up at least one bait, and five of them showed detectable antibody levels seven weeks after vaccination. These results were supplemented by stability tests. Appropriate stability of vaccine virus was shown both under field and laboratory conditions. In total, most results were in line with our expectations. However, optimization of the DIVA assay has to be attempted in the future.

  • Chimeric classical swine fever (CSF)-Japanese encephalitis (JE) viral replicon as a non-transmissible vaccine candidate against CSF and JE infections.

    Science.gov (United States)

    Yang, Zhenhua; Wu, Rui; Li, Robert W; Li, Ling; Xiong, Zhongliang; Zhao, Haizhong; Guo, Deyin; Pan, Zishu

    2012-04-01

    A trans-complemented chimeric CSF-JE virus replicon was constructed using an infectious cDNA clone of the CSF virus (CSFV) Alfort/187 strain. The CSFV E2 gene was deleted, and a fragment containing the region encoding a truncated envelope protein (tE, amino acid 292-402, domain III) of JE virus (JEV) was inserted into the resultant plasmid, pA187delE2, to generate the recombinant cDNA clone pA187delE2/JEV-tE. Porcine kidney 15 (PK15) cells that constitutively express the CSFV E2p7 proteins were then transfected with in vitro-transcribed RNA from pA187delE2/JEV-tE. As a result, the chimeric CSF-JE virus replicon particle (VRP), rv187delE2/JEV-tE, was rescued. In a mouse model, immunization with the chimeric CSF-JE VRP induced strong production of JEV-specific antibody and conferred protection against a lethal JEV challenge. Pigs immunized with CSF-JE VRP displayed strong anti-CSFV and anti-JEV antibody responses and protection against CSFV and JEV challenge infections. Our evidence suggests that E2-complemented CSF-JE VRP not only has potential as a live-attenuated non-transmissible vaccine candidate against CSF and JE but also serves as a potential DIVA (Differentiating Infected from Vaccinated Animals) vaccine for CSF in pigs. Together, our data suggest that the non-transmissible chimeric VRP expressing foreign antigenic proteins may represent a promising strategy for bivalent DIVA vaccine design. Copyright © 2012 Elsevier B.V. All rights reserved.

  • Diagnósticos de enfermagem respiratórios para crianças com infecção respiratória aguda Diagnósticos de enfermería respiratorios para niños con infección respiratoria aguda Respiratory nursing diagnoses for children with acute respiratory infection

    Directory of Open Access Journals (Sweden)

    Lívia Zulmyra Cintra Andrade

    2012-01-01

    Full Text Available OBJETIVOS: Identificar a prevalência dos diagnósticos de enfermagem: Padrão respiratório ineficaz (PRI, Desobstrução ineficaz de vias aéreas (DIVA, Troca de gases prejudicada (TGP e Ventilação espontânea prejudicada (VEP suas características definidoras e fatores relacionados, em crianças com infecção respiratória aguda. MÉTODOS: Estudo de abordagem quantitativa, transversal, com 151 crianças. Os dados foram coletados por meio de entrevista e de avaliação pulmonar. Para análise dos dados, foi utilizada a estatística descritiva e inferencial. RESULTADOS: O diagnóstico mais prevalente foi PRI. Ruídos adventícios respiratórios e tosse ineficaz foram às características definidoras mais prevalentes. Ruídos adventícios respiratórios aumentam em 80% a chance para DIVA. Entre os fatores relacionados, destacaram-se: secreções nos brônquios e secreções retidas. Secreções nos brônquios favorecem em 80% a ocorrência de PRI e em 60% de DIVA. Verificou-se associação estatisticamente significante entre as características e fatores relacionados com os diagnósticos estudados. CONCLUSÃO: Os quatro diagnósticos foram identificados em crianças com infecções respiratórias agudas, apresentando prevalências distintas.OBJETIVOS: Identificar la prevalencia de los diagnósticos de enfermería: Patrón respiratorio ineficaz (PRI, Desobstrucción ineficaz de vías aéreas (DIVA, Intercambio de gases perjudicada (IGP y Ventilación espontánea perjudicada (VEP sus características definidoras y factores relacionados, en niños con infección respiratoria aguda. MÉTODOS: Estudio de abordaje cuantitativo, transversal, realizado con 151 niños. Los datos fueron recolectados por medio de entrevista y de evaluación pulmonar. Para el análisis de los datos, fue utilizada la estadística descriptiva e inferencial. RESULTADOS: El diagnóstico más prevalente fue el PRI. Los ruidos adventicios respiratorios y tos ineficaz fueron

  • Les transformations de l'image de Saladin dans les sources occidentales

    Directory of Open Access Journals (Sweden)

    Jean Richard

    2009-05-01

    Full Text Available Le personnage de Salāh al-din Yūsuf, officier kurde au service de Nūr al-dïn devenu maître de l'Égypte puis de Damas et enfin d'Alep, et fondateur de l'empire ayyoubide, a été fort controversé de son vivant avant de devenir pour la postérité « le plus pur des héros de l'Islam ». Pour ses adversaires, les Francs d'Orient, il a de bonne heure suscité leur curiosité, et ceux-ci ont recueilli des informations qu'ils ont utilisées, dès avant 1187, dans un Carmen de Saladino où l'aventurier qu'il était à leurs yeux est présenté sous des traits peu recommandables. Son manque de fidélité envers ses maîtres est particulièrement souligné. Les coups très durs qu'il a portés aux Francs ont été assortis de traits de cruauté ; mais ce sont les manifestations d'un esprit chevaleresque, dont les valeurs étaient communes aux deux camps, qui ont vite retenu l'attention. Le trouvère Ambroise le regarde encore comme un ennemi fort capable de perfidie ; mais le traducteur de Guillaume de Tyr adoucit les remarques du livre qu'il mettait en français pour le rendre plus sympathique. Les textes de caractère épique où il apparaît dans la première moitié du XIIIe siècle (Estoires d'Outremer, Ordene de Chevalerie accentuent ce caractère. Mais c'est dans le second cycle de la croisade qu'on le pare des qualités les plus éminentes qu'on attend d'un chevalier. On lui prête des origines chrétiennes (La fille du comte de Ponthieu et on lui attribue le beau rôle, en insistant sur sa générosité et sur sa bravoure, en lui faisant arbitrer des controverses religieuses, en lui prêtant enfin une aspiration à une mort chrétienne, qui sont bien loin du portrait que faisaient de lui les Francs de la fin du XIIe siècle.

  • Use of M2e ELISAs for longitudinal surveillance of commercial poultry in Indonesia vaccinated against highly pathogenic avian influenza.

    Science.gov (United States)

    Wibowo, Michael Haryadi; Tarigan, Simson; Sumarningsih; Artanto, Sidna; Indriani, Risa; Anggoro, Dito; Putra, Cahyaditya Pratama; Idris, Syafrison; Untari, Tri; Asmara, Widya; Tabbu, Charles Rangga; Ignjatovic, Jagoda

    2017-11-01

    In countries where highly pathogenic avian influenza virus (HPAIV) H5N1 is endemic and controlled by vaccination, post-vaccination serological monitoring is essential to differentiate vaccinated poultry from those that are infected. The objectives of this study were to validate two experimental ELISAs that detect antibodies raised against the M2e protein of avian influenza virus that can be used for DIVA purposes. Results from the sM2e and tM2e ELISAs were compared with other conventional tests for the detection of H5N1influenza virus (virus isolation and RT-PCR) using samples collected from 16 commercial flocks in Indonesia. These comprised vaccinated layers aged between 18 and 68 weeks old that were sampled at ten-weekly intervals. A small number of sera were positive in sM2e and tM2e ELISA, 14 (0.6%) and 17 (0.7%) respectively, with low OD420 (0.1-0.3), but only 4 sera were positive in both tests. At the flock level, the incidence of M2e positive sera was low (4%), well below previously established minimum of 40% for an HPAIV H5N1-infected flock. Conventional M and H5 gene RT-PCRs indicated that none of 16 flocks were infected at any time during the study. No virus was isolated from any of the 480 pooled swab samples, except from one, for which the combined data analysis suggest to be the result of a laboratory cross-contamination. Clinical disease, mortalities or reduction in production performance, indicative of field H5N1 challenge, were not observed either in any of the flocks. Birds from two surveyed flocks, challenged in the laboratory with an Indonesian HPAIV H5N1 developed M2e antibodies in 50% and 55% of surviving birds with OD420 in the range of 0.35-1.47 in tM2e ELISA, confirming the validity of the criteria established for use of M2e ELISA for DIVA purposes. Overall these results showed that the tM2e ELISA could be a useful monitoring tool to ascertain freedom from H5N1 infections in vaccinated commercial poultry. Copyright © 2017 Elsevier B.V. All

    1. Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.

      Science.gov (United States)

      Blodörn, Krister; Hägglund, Sara; Fix, Jenna; Dubuquoy, Catherine; Makabi-Panzu, Boby; Thom, Michelle; Karlsson, Per; Roque, Jean-Louis; Karlstam, Erika; Pringle, John; Eléouët, Jean-François; Riffault, Sabine; Taylor, Geraldine; Valarcher, Jean François

      2014-01-01

      The development of safe and effective vaccines against both bovine and human respiratory syncytial viruses (BRSV, HRSV) to be used in the presence of RSV-specific maternally-derived antibodies (MDA) remains a high priority in human and veterinary medicine. Herein, we present safety and efficacy results from a virulent BRSV challenge of calves with MDA, which were immunized with one of three vaccine candidates that allow serological differentiation of infected from vaccinated animals (DIVA): an SH gene-deleted recombinant BRSV (ΔSHrBRSV), and two subunit (SU) formulations based on HRSV-P, -M2-1, and -N recombinant proteins displaying BRSV-F and -G epitopes, adjuvanted by either oil emulsion (Montanide ISA71VG, SUMont) or immunostimulating complex matrices (AbISCO-300, SUAbis). Whereas all control animals developed severe respiratory disease and shed high levels of virus following BRSV challenge, ΔSHrBRSV-immunized calves demonstrated almost complete clinical and virological protection five weeks after a single intranasal vaccination. Although mucosal vaccination with ΔSHrBRSV failed to induce a detectable immunological response, there was a rapid and strong anamnestic mucosal BRSV-specific IgA, virus neutralizing antibody and local T cell response following challenge with virulent BRSV. Calves immunized twice intramuscularly, three weeks apart with SUMont were also well protected two weeks after boost. The protection was not as pronounced as that in ΔSHrBRSV-immunized animals, but superior to those immunized twice subcutaneously three weeks apart with SUAbis. Antibody responses induced by the subunit vaccines were non-neutralizing and not directed against BRSV F or G proteins. When formulated as SUMont but not as SUAbis, the HRSV N, P and M2-1 proteins induced strong systemic cross-protective cell-mediated immune responses detectable already after priming. ΔSHrBRSV and SUMont are two promising DIVA-compatible vaccines, apparently inducing protection by

    2. 常春油麻藤地理分布分析和应用研究%Geographic Distribution and Application of Mucuna sempervirens Hems

      Institute of Scientific and Technical Information of China (English)

      董运常

      2012-01-01

      常春油麻藤是豆科黧豆属常绿木质藤本植物.该研究运用Diva-GIS软件,结合海拔高度图层和植被图层绘制了常春油麻藤的地理分布图,分析了常春油麻藤的分布规律和生境特性.结果表明,常春油麻藤在中国34.5°N以南中低海拔(300~3 000 m)地区均有分布,如亚热带森林、灌木丛、溪谷、河边和山坡等;在浙江、广东、广西、四川、湖北、云南、贵州、湖南、江西、陕西、甘肃、福建、安徽等省区及其交界处均有分布;其分布与植被类型和海拔有着密切的关系,分布区的植被类型为亚热带常绿阔叶林和热带季雨林.常春油麻藤作为多用途的园林植物具有较高的药用观赏价值.%Mucuna sempervirens Hems L. Belongs to Mucuna of Leguminosae, which is an evergreen woody vine. By using the Diva-GIS software , the geographic distribution maps of M. Sempervirens were established based on the altitude layer and vegetation layer. The distribution rule and habitat characteristics of M. Sempervirens were analyzed. Results showed that M. Sempervirens was distributed below 34.5 °N with the altitude of 300-3 000 m in China,such as the subtropical forests,bushes,streams and hillsides. It grew in Zhejiang,Guangdong,Guangxi,Sichuan, Hubei, Yunnan, Guizhou, Hunan, Jiangxi,Shaanxi,Gansu,Fujian and Anhui Provinces of China. Its geographical distribution and vegetation type were closed affected by the altitude. The vegetation types in distribution region were mainly subtropical evergreen broadleaved forest and tropical seasonal rain forest. As an all-purpose ornamental plant,M. Sempervirens had relatively high medicinal value and ornamental value.

    3. Ação coletiva no âmbito da economia solidária e da autogestão Collective action in the realm of solidarity economics and self-management

      Directory of Open Access Journals (Sweden)

      Maria Eugênia Monteiro Castanheira

      2008-06-01

      Full Text Available Este ensaio traz reflexões sobre as motivações que conduzem a ação coletiva no âmbito da economia solidária e da autogestão, partindo do pressuposto de que, nestas esferas, a ação coletiva se diferencia daquela que se desenvolve nos domínios do capitalismo e daquela que se desenvolve no contexto social das relações tradicionais. Neste sentido, o objetivo deste ensaio é explorar as especificidades teóricas da ação coletiva orientada pelos princípios da economia solidária e da autogestão, tendo como enfoque analítico a teoria da dádiva. Para tanto, procurou-se, de forma articulada e seqüencial, problematizar a economia solidária, evidenciar os elementos constitutivos da autogestão e o aporte teórico da teoria da dádiva. Acredita-se que tais ponderações indicam um campo a ser explorado empiricamente, podendo contribuir para a elaboração de novos estudos e pesquisas sobre este tema.This essay reflects on the motivations that lead to collective action in the realm of solidarity economics and self-management, based on the presumption that, in these spheres, collective action is different from that which takes places in the domains of capitalism and that which develops in the social context of traditional relations. In this sense, the purpose of this essay is to explore the theoretical specificities of collective action guided by the principles of solidarity economics and self-management, with an analytical focus on the theory of donation. To do so, it sought to analyze solidarity economics in an articulated and sequential manner, to reveal the constituent elements of self management and the theoretical support for the theory of donation. These considerations indicate that there is much empirical research to do in this field, and such research can contribute to the elaboration of new studies and research about this issue.

    4. Anna Pavlova, danzatrice eurasiana? (I parte

      Directory of Open Access Journals (Sweden)

      Silvia Mei

      2010-12-01

      Full Text Available Abstract – IT Prima diva nella costellazione di nuove star dello spettacolo novecentesco, Anna Pavlova visse unicamente nello spirito e nella missione della danza come forma d’arte e stile di vita. Senza temere i rischi del solipsismo, si lancia nell’avventura di una compagnia tutta sua dopo il clamore parigino dei Ballets Russes. Il suo coraggio, la sua curiosità, il suo genio artistico la portano a viaggiare per i cinque continenti senza sosta, a scoprire danze tradizionali, a praticarle essa stessa, come quelle kabuki, apprese non senza sofferenze in Giappone nel 1922. Presentata nella vulgata come paladina della tradizione classicoaccademica, Pavlova può ostentare una biografia degna delle grandi danzatrici moderne a lei contemporanee, come una Duncan o una Ruth St. Denis (con quest’ultima in rapporto di competizione a distanza intorno al soggetto di Radha. Insieme ad una figura controversa e intrigante come quella del danzatore indiano Uday Shankar, crea un trittico di danze di ispirazione orientale all’insegna del rigore filologico e dell’integrazione linguistica, traccia della sua apertura e del rispetto di tradizioni teatrali altre. Abstract – EN Anna Pavlova, the first diva within the stars firmament of the 20th century, spent her life exclusively in the spirit of dance, intended as an art form and a life style, as if it were a mission. She threw herself into the adventure of leading her own company, after the Ballets Russes' European acclaim. Her courage, curiosity and artistic genius took her through five continents, endlessly searching for traditional dances that she learned with tremendous physical suffering, as in Japan with kabuki in 1922. She’s not simply a paladin of the academic tradition. She can in fact claim a biography and dance practice worthy of the most famous modern dancers who lived in her age, such as Duncan and St. Denis (with whom she was in a special competition, though after years, on the subject

    5. The Casiquiare river acts as a corridor between the Amazonas and Orinoco river basins: biogeographic analysis of the genus Cichla.

      Science.gov (United States)

      Willis, S C; Nunes, M; Montaña, C G; Farias, I P; Ortí, G; Lovejoy, N R

      2010-03-01

      The Casiquiare River is a unique biogeographic corridor between the Orinoco and Amazonas basins. We investigated the importance of this connection for Neotropical fishes using peacock cichlids (Cichla spp.) as a model system. We tested whether the Casiquiare provides a conduit for gene flow between contemporary populations, and investigated the origin of biogeographic distributions that span the Casiquiare. Using sequences from the mitochondrial control region of three focal species (C. temensis, C. monoculus, and C. orinocensis) whose distributions include the Amazonas, Orinoco, and Casiquiare, we constructed maximum likelihood phylograms of haplotypes and analyzed the populations under an isolation-with-migration coalescent model. Our analyses suggest that populations of all three species have experienced some degree of gene flow via the Casiquiare. We also generated a mitochondrial genealogy of all Cichla species using >2000 bp and performed a dispersal-vicariance analysis (DIVA) to reconstruct the historical biogeography of the genus. This analysis, when combined with the intraspecific results, supports two instances of dispersal from the Amazonas to the Orinoco. Thus, our results support the idea that the Casiquiare connection is important across temporal scales, facilitating both gene flow and the dispersal and range expansion of species.

    6. Grafiskā stāstījuma uzbūve un trauma Hāleda Hoseinī grafiskajā romānā “Pūķa ķērājs”

      OpenAIRE

      2016-01-01

      Hāleda Hoseinī grafiskais romāns “Pūķa ķērājs”demonstrē grafisko romānu spēju vienlaicīgi uzrunāt lielu cilvēku daudzumu un tajā pašā laikā izteikt neaprakstāmo, kas apliecina to, ka grafiskie romāni ļoti labi prot darīt saprotamas tādas neaprakstāmas tēmas, kā trauma. Tādēļ, šī maģistra darba mērķis ir noteikt, kā grafiskā stāstījuma uzbūve attēlo traumu Hāleda Hoseinī grafiskajā romāna “Pūķa ķērājs” un kādu rezultātu tas rada. Iepriekš minētā mērķa sasniegšanai tika izvēlētas divas pētīšana...

    7. Role of the auditory system in speech production.

      Science.gov (United States)

      Guenther, Frank H; Hickok, Gregory

      2015-01-01

      This chapter reviews evidence regarding the role of auditory perception in shaping speech output. Evidence indicates that speech movements are planned to follow auditory trajectories. This in turn is followed by a description of the Directions Into Velocities of Articulators (DIVA) model, which provides a detailed account of the role of auditory feedback in speech motor development and control. A brief description of the higher-order brain areas involved in speech sequencing (including the pre-supplementary motor area and inferior frontal sulcus) is then provided, followed by a description of the Hierarchical State Feedback Control (HSFC) model, which posits internal error detection and correction processes that can detect and correct speech production errors prior to articulation. The chapter closes with a treatment of promising future directions of research into auditory-motor interactions in speech, including the use of intracranial recording techniques such as electrocorticography in humans, the investigation of the potential roles of various large-scale brain rhythms in speech perception and production, and the development of brain-computer interfaces that use auditory feedback to allow profoundly paralyzed users to learn to produce speech using a speech synthesizer.

    8. Molecular phylogeny of the harvestmen genus Sabacon (Arachnida: Opiliones: Dyspnoi) reveals multiple Eocene-Oligocene intercontinental dispersal events in the Holarctic.

      Science.gov (United States)

      Schönhofer, Axel L; McCormack, Maureen; Tsurusaki, Nobuo; Martens, Jochen; Hedin, Marshal

      2013-01-01

      We investigated the phylogeny and biogeographic history of the Holarctic harvestmen genus Sabacon, which shows an intercontinental disjunct distribution and is presumed to be a relatively old taxon. Molecular phylogenetic relationships of Sabacon were estimated using multiple gene regions and Bayesian inference for a comprehensive Sabacon sample. Molecular clock analyses, using relaxed clock models implemented in BEAST, are applied to date divergence events. Biogeographic scenarios utilizing S-DIVA and Lagrange C++ are reconstructed over sets of Bayesian trees, allowing for the incorporation of phylogenetic uncertainty and quantification of alternative reconstructions over time. Four primary well-supported subclades are recovered within Sabacon: (1) restricted to western North America; (2) eastern North American S. mitchelli and sampled Japanese taxa; (3) a second western North American group and taxa from Nepal and China; and (4) eastern North American S. cavicolens with sampled European Sabacon species. Three of four regional faunas (wNA, eNA, East Asia) are thereby non-monophyletic, and three clades include intercontinental disjuncts. Molecular clock analyses and biogeographic reconstructions support nearly simultaneous intercontinental dispersal coincident with the Eocene-Oligocene transition. We hypothesize that biogeographic exchange in the mid-Tertiary is likely correlated with the onset of global cooling, allowing cryophilic Sabacon taxa to disperse within and among continents. Morphological variation supports the divergent genetic clades observed in Sabacon, and suggests that a taxonomic revision (e.g., splitting Sabacon into multiple genera) may be warranted.

    9. Current developments in avian influenza vaccines, including safety of vaccinated birds as food.

      Science.gov (United States)

      Swayne, D E; Suarez, D L

      2007-01-01

      Until recently, most vaccines against avian influenza were based on oil-emulsified inactivated low- or high-pathogenicity viruses. Now, recombinant fowl pox and avian paramyxovirus type 1 vaccines with avian influenza H5 gene inserts (+ or - N1 gene insert) are available and licensed. New technologies might overcome existing limitations to make available vaccines that can be grown in tissue culture systems for more rapid production; provide optimized protection, as a result of closer genetic relations to field viruses; allow mass administration by aerosol, in drinking-water or in ovo; and allow easier strategies for identifying infected birds within vaccinated populations (DIVA). The technologies include avian influenza viruses with partial gene deletions, avian influenza-Newcastle disease virus chimeras, vectored vaccines such as adenoviruses and Marek's disease virus, and subunit vaccines. These new methods should be licensed only after their purity, safety, efficacy and potency against avian influenza viruses have been demonstrated, and, for live vectored vaccines, restriction of viral transmission to unvaccinated birds. Use of vaccines in countries affected by highly pathogenic avian influenza will not only protect poultry but will provide additional safety for consumers. Experimental studies have shown that birds vaccinated against avian influenza have no virus in meat and minimal amounts in eggs after HPAI virus challenge, and that replication and shedding from their respiratory and alimentary tracts is greatly reduced.

    10. Overreliance on auditory feedback may lead to sound/syllable repetitions: simulations of stuttering and fluency-inducing conditions with a neural model of speech production

      Science.gov (United States)

      Civier, Oren; Tasko, Stephen M.; Guenther, Frank H.

      2010-01-01

      This paper investigates the hypothesis that stuttering may result in part from impaired readout of feedforward control of speech, which forces persons who stutter (PWS) to produce speech with a motor strategy that is weighted too much toward auditory feedback control. Over-reliance on feedback control leads to production errors which, if they grow large enough, can cause the motor system to “reset” and repeat the current syllable. This hypothesis is investigated using computer simulations of a “neurally impaired” version of the DIVA model, a neural network model of speech acquisition and production. The model’s outputs are compared to published acoustic data from PWS’ fluent speech, and to combined acoustic and articulatory movement data collected from the dysfluent speech of one PWS. The simulations mimic the errors observed in the PWS subject’s speech, as well as the repairs of these errors. Additional simulations were able to account for enhancements of fluency gained by slowed/prolonged speech and masking noise. Together these results support the hypothesis that many dysfluencies in stuttering are due to a bias away from feedforward control and toward feedback control. PMID:20831971

    11. Anizekonijas izmeklēšanas un optiskās korekcijas metodes

      OpenAIRE

      Amato, Luigi

      2013-01-01

      Šis bakalaura darbs ir uzrakstīts angļu valodā uz 26 drukātām lapām, tas satur 15 zīmējumus, 2 tabulas un 31 bibliogrāfijas avotus. Darba galvenais mērķis ir anizeikonijas pētījumi. Anizeikonija ir binokulārais defekts, kas ir saistīts ar attēlu, kas nonāk smadzenēs, atšķirību (1). Divas acis tad uztver atšķirīgus attēlus. Klīniski būtiku anizeikoniju ( attēlu izmēru atšķirība virs 2%) novēro apmēram 1-3,5% iedzīvotāju (2). Sākotnēji anizeikonija parādās pacientiem ar anizometropiju vai an...

    12. Chemodiversity of Epicuticular n-Alkanes and Morphological Traits of Natural Populations of Satureja subspicata Bartl. ex Vis. along Dinaric Alps - Ecological and Taxonomic Aspects.

      Science.gov (United States)

      Dodoš, Tanja; Rajčević, Nemanja; Tešević, Vele; Marin, Petar D

      2017-02-01

      Morphological characters and the composition of epicuticular leaf n-alkanes of two Satureja subspicata Bartl. ex Vis. subspecies (subsp. liburnica Šilić and subsp. subspicata) from nine natural populations along Dinaric Alps range were studied. Morphological characters were chosen based on Šilić's subspecies separation. Seventeen n-alkane homologues (C19 - C35 ) were identified using gas chromatography/mass spectrometry (GC/MS) and GC/flame ionisation detector (FID). The most abundant n-alkane in all populations was n-nonacosane (C29 ), followed by n-hentriacontane (C31 ), with the exception of Divača population where these two alkanes were co-dominant. Diversity and variability of n-alkane patterns and morphological characters and their relation to different geographic and bioclimatic parameters, including exposure, were analysed by several statistical multivariate methods (PCA, HCA, Discriminant Analysis, Mantel test). These tests showed clear separation of subsp. liburnica from subsp. subspicata, even though population Velebit showed separation from other subsp. liburnica populations based on phytochemical characters. Mantel test showed high correlation with geographical distribution in both investigated data sets. High correlation between morphological and phytochemical characters was also established. However, exposure can influence n-alkane profile, suggesting precaution while taking samples from natural habitats.

    13. Outreach programmes to attract girls into computing: how the best laid plans can sometimes fail

      Science.gov (United States)

      Lang, Catherine; Fisher, Julie; Craig, Annemieke; Forgasz, Helen

      2015-07-01

      This article presents a reflective analysis of an outreach programme called the Digital Divas Club. This curriculum-based programme was delivered in Australian schools with the aim of stimulating junior and middle school girls' interest in computing courses and careers. We believed that we had developed a strong intervention programme based on previous literature and our collective knowledge and experiences. While it was coordinated by university academics, the programme content was jointly created and modified by practicing school teachers. After four years, when the final data were compiled, it showed that our programme produced significant change to student confidence in computing, but the ability to influence a desire to pursue a career path in computing did not fully eventuate. To gain a deeper insight in to why this may be the case, data collected from two of the schools are interrogated in more detail as described in this article. These schools were at the end of the expected programme outcomes. We found that despite designing a programme that delivered a multi-layered positive computing experience, factors beyond our control such as school culture and teacher technical self-efficacy help account for the unanticipated results. Despite our best laid plans, the expectations that this semester long programme would influence students' longer term career outcomes may have been aspirational at best.

    14. Future directions in Alzheimer's disease from risk factors to prevention.

      Science.gov (United States)

      Imtiaz, Bushra; Tolppanen, Anna-Maija; Kivipelto, Miia; Soininen, Hilkka

      2014-04-15

      The increase in life expectancy has resulted in a high occurrence of dementia and Alzheimer's disease (AD). Research on AD has undergone a paradigm shift from viewing it as a disease of old age to taking a life course perspective. Several vascular, lifestyle, psychological and genetic risk factors influencing this latent period have been recognized and they may act both independently and by potentiating each other. These risk factors have consequently been used to derive risk scores for predicting the likelihood of dementia. Despite population differences, age, low education and vascular risk factors were identified as key factors in all scoring systems. Risk scores can help to identify high-risk individuals who might benefit from different interventions. The European Dementia Prevention Initiative (EDPI), an international collaboration, encourages data sharing between different randomized controlled trials. At the moment, it includes three large ongoing European trials: Finnish Geriatric Intervention Study to Prevent Cognitive Impairment and Disability (FINGER), Prevention of Dementia by Intensive Vascular Care (preDIVA), and Multidomain Alzheimer Prevention study (MAPT). Recently EDPI has developed a "Healthy Aging through Internet Counseling in Elderly" (HATICE) program, which intends to manage modifiable risk factors in an aged population through an easily accessible Internet platform. Thus, the focus of dementia research has shifted from identification of potential risk factors to using this information for developing interventions to prevent or delay the onset of dementia as well as identifying special high-risk populations who could be targeted in intervention trials.

    15. The spatial and temporal distribution of microalgae in the South China Sea:evidence from GIS-based analysis of 18S rDNA sequences

      Institute of Scientific and Technical Information of China (English)

      2008-01-01

      The purpose of this study was to estimate the spatial and temporal variation of microalgae in the South China Sea and to demonstrate the environmental factors controlling the diversity of microalgae by GIS (geographic information system)-based analysis of 18S rDNA sequences. Six 18S rDNA libraries were constructed from environmental samples collected at different sites in the study area, and more than 600 18S rDNA sequences were determined. The rDNA sequence data were then analyzed by DIVA-GIS software to display the spatial and temporal variation of phytoplankton’s composition. It was shown that the autotrophic eukaryotic plankton dominated over the heterotrophic cells in most of our clone libraries, and the dominating phytoplankton was Dinophyceae except for Bacillariophyta at the Xiamen harbor. The percentages of these two groups were controlled by water temperature and salinity. Our results also revealed that the species composition of Chlorophyta showed a close relationship with latitude, changing from Prasinophyceae at the high latitude to Trebouxiophyceae at the low latitude. Several newly classified picoplankton lineages were first uncovered in the South China Sea, including the pico-sized green alga Ostreococcus sp. and Picochlorum eukaryotum, and picobiliphytes, which was just discovered in 2007 with unknown affinities to other eukaryotes. Their spatial and temporal variation were also analyzed and discussed.

    16. Desafio da Vida: trabalho, velhice e memória

      Directory of Open Access Journals (Sweden)

      Hebréia Maria Ramos Barbosa da Costa

      2012-04-01

      Full Text Available <div>As necessidades do desenvolvimento capitalista no Brasil exigiram um contingente significativo de trabalhadores para sua expansão e reprodução. Este elemento é vital para compreender o aumento populacional e o rápido envelhecimento dos trabalhadores objeto de interesse da pesquisa que embasa este artigo. A despeito dos avanços científicos, alcançar a longevidade implica valentia para enfrentar um “sistema” cuja finalidade precípua é a apropriação da mais-valia. Nesse sentido, essa investigação rechaça as ideologias que destituem de historicidade a velhice, travestindo-a em mero objeto da natureza. Recupera-se a centralidade do trabalho como elemento fundante e humanizador do indivíduo social, por conseguinte, a noção de classe social, segundo a compreensão de Marx. Os resultados alcançados no estudo permitem compreender o velho não como segmento, mas como membro da classe trabalhadora, e nesse movimento teórico-metodológico é que se vislumbra a

    importância de trazer à tona o fio que o une aos demais companheiros de classe.

  • Application of reverse genetics for producing attenuated vaccine strains against highly pathogenic avian influenza viruses.

    Science.gov (United States)

    Uchida, Yuko; Takemae, Nobuhiro; Saito, Takehiko

    2014-08-01

    In this study, reverse genetics was applied to produce vaccine candidate strains against highly pathogenic avian influenza viruses (HPAIVs) of the H5N1 subtype. The H5 subtype vaccine strains were generated by a reverse genetics method in a biosafety level 2 facility. The strain contained the HA gene from the H5N1 subtype HPAIV attenuated by genetic modification at the cleavage site, the NA gene derived from the H5N1 subtype HPAI or the H5N3 subtype of avian influenza virus and internal genes from A/Puerto Rico/8/34. Vaccination with an inactivated recombinant virus with oil-emulsion completely protected chickens from a homologous viral challenge with a 640 HAU or 3,200 HAU/vaccination dose. Vaccination with a higher dose of antigen, 3,200 HAU, was effective at increasing survival and efficiently reduced viral shedding even when challenged by a virus of a different HA clade. The feasibility of differentiation of infected from vaccinated animals (DIVA) was demonstrated against a challenge with H5N1 HPAIVs when the recombinant H5N3 subtype viruses were used as the antigens of the vaccine. Our study demonstrated that the use of reverse genetics would be an option to promptly produce an inactivated vaccine with better matching of antigenicity to a circulating strain.

  • Vesicular stomatitis virus replicon expressing the VP2 outer capsid protein of bluetongue virus serotype 8 induces complete protection of sheep against challenge infection.

    Science.gov (United States)

    Kochinger, Stefanie; Renevey, Nathalie; Hofmann, Martin A; Zimmer, Gert

    2014-06-13

    Bluetongue virus (BTV) is an arthropod-borne pathogen that causes an often fatal, hemorrhagic disease in ruminants. Different BTV serotypes occur throughout many temperate and tropical regions of the world. In 2006, BTV serotype 8 (BTV-8) emerged in Central and Northern Europe for the first time. Although this outbreak was eventually controlled using inactivated virus vaccines, the epidemic caused significant economic losses not only from the disease in livestock but also from trade restrictions. To date, BTV vaccines that allow simple serological discrimination of infected and vaccinated animals (DIVA) have not been approved for use in livestock. In this study, we generated recombinant RNA replicon particles based on single-cycle vesicular stomatitis virus (VSV) vectors. Immunization of sheep with infectious VSV replicon particles expressing the outer capsid VP2 protein of BTV-8 resulted in induction of BTV-8 serotype-specific neutralizing antibodies. After challenge with a virulent BTV-8 strain, the vaccinated animals neither developed signs of disease nor showed viremia. In contrast, immunization of sheep with recombinant VP5 - the second outer capsid protein of BTV - did not confer protection. Discrimination of infected from vaccinated animals was readily achieved using an ELISA for detection of antibodies against the VP7 antigen. These data indicate that VSV replicon particles potentially represent a safe and efficacious vaccine platform with which to control future outbreaks by BTV-8 or other serotypes, especially in previously non-endemic regions where discrimination between vaccinated and infected animals is crucial.

  • [Predicting the impact of global warming on the geographical distribution pattern of Quercus variabilis in China].

    Science.gov (United States)

    Li, Yao; Zhang, Xing-wang; Fang, Yan-ming

    2014-12-01

    The geographical distribution of Quercus variabilis in China with its climate characteristics was analyzed based on DIVA-GIS which was also used to estimate the response of future potential distribution to global warming by Bioclim and Domain models. Analysis results showed the geographical distribution of Q. variabilis could be divided into 7 subregions: Henduan Mountains, Yunnan-Guizhou Plateau, North China, East China, Liaodong-Shandong Peninsula, Taiwan Island, and Qinling-Daba Mountains. These subregions are across 7 temperature zones, 2 moisture regions and 17 climatic subregions, including 8 climate types. The modern abundance center of Q. variabilis is Qinling, Daba and Funiu mountains. The condition of mean annual temperature 7.5-19.8 degrees C annual precipitation 471-1511 mm, is suitable for Q. variabilis. Areas under the receiver operating characteristic curve (AUC values), of Domain and Boiclim models were 0.910, 0.779; the former predicted that the potential regions of high suitability for Q. variabilis are Qinling, Daba, Funiu, Tongbai, and Dabie mountains, eastern and western Yunnan-Guizhou Plateau, hills of southern Jiangsu and Anhui, part of the mountains in North China. Global warming might lead to the shrinking in suitable region and retreating from the south for Q. variabilis.

  • A new species of Tambja (Mollusca, Gastropoda, Nudibranchia) from the Mediterranean Sea: description of the first species of the genus from the Balearic Islands and Malta

    Science.gov (United States)

    Domínguez, M.; Pola, M.; Ramón, M.

    2015-06-01

    A new species of polycerid nudibranchs of the genus Tambja is described from Mallorca Island (Spain) and Malta. So far, only two species of Tambja had been recorded in the Mediterranean Sea with a distribution limited to southern Spain. With Tambja mediterranea sp. nov., the distribution of the genus in the Mediterranean Sea is extended, and the new species represents the first occurrence of Tambja at the Balearic Islands and Malta. Externally, the new species is mainly characterized by having ground orange-red colour, dorsum covered with rounded whitish tubercles, rhinophores red with whitish tips and three gill branches with orange-reddish rachis and whitish branches. In the present paper, external and internal features of T. mediterranea are described and compared with other species of the genus, especially with its most similar species, T. limaciformis. Additionally, phylogenetic analyses (Bayesian and maximum likelihood) based on mitochondrial sequences (COI) show that T. mediterranea sp. nov. is sister to T. divae and that both species cluster together with T. limaciformis and T. amakusana with the maximum support.

    1. Early evolution and historical biogeography of fishflies (Megaloptera: Chauliodinae: implications from a phylogeny combining fossil and extant taxa.

      Directory of Open Access Journals (Sweden)

      Xingyue Liu

      Full Text Available Fishflies (Corydalidae: Chauliodinae are one of the main groups of the basal holometabolous insect order Megaloptera, with ca. 130 species distributed worldwide. A number of genera from the Southern Hemisphere show remarkably disjunctive distributions and are considered to be the austral remnants or "living fossils" of Gondwana. Hitherto, the evolutionary history of fishflies remains largely unexplored due to limited fossil record and incomplete knowledge of phylogenetic relationships. Here we describe two significant fossil species of fishflies, namely Eochauliodes striolatus gen. et sp. nov. and Jurochauliodes ponomarenkoi Wang & Zhang, 2010 (original designation for fossil larvae only, from the Middle Jurassic of Inner Mongolia, China. These fossils represent the earliest fishfly adults. Furthermore, we reconstruct the first phylogenetic hypothesis including all fossil and extant genera worldwide. Three main clades within Chauliodinae are recognized, i.e. the Dysmicohermes clade, the Protochauliodes clade, and the Archichauliodes clade. The phylogenetic and dispersal-vicariance (DIVA analyses suggest Pangaean origin and global distribution of fishflies before the Middle Jurassic. The generic diversification of fishflies might have happened before the initial split of Pangaea, while some Gondwanan-originated clades were likely to be affected by the sequential breakup of Pangaea. The modern fauna of Asian fishflies were probably derived from their Gondwanan ancestor but not the direct descendents of the Mesozoic genera in Asia.

    2. Molecular phylogeny and biogeography of the weevil subfamily Platypodinae reveals evolutionarily conserved range patterns.

      Science.gov (United States)

      Jordal, Bjarte H

      2015-11-01

      Platypodinae is a peculiar weevil subfamily of species that cultivate fungi in tunnels excavated in dead wood. Their geographical distribution is generally restricted, with genera confined to a single continent or large island, which provides a useful system for biogeographical research. This study establishes the first detailed molecular phylogeny of the group, with the aim of testing hypotheses on classification, diversification, and biogeography. A phylogeny was reconstructed based on 3648 nucleotides from COI, EF-1α, CAD, ArgK, and 28S. Tree topology was well resolved and indicated a strong correlation with geography, more so than predicted by previous morphology-based classifications. Tesserocerini was paraphyletic, with Notoplatypus as the sister group to a clade consisting of three main lineages of Tesserocerini and the recently evolved Platypodini. Austroplatypus formed the sister group to all remaining Platypodini and hence confirmed its separate status from Platypus. The Indo-Australian genera of Platypodini were strikingly paraphyletic, suggesting that the taxonomy of this tribe needs careful revision. Ancestral-area reconstructions in Lagrange and S-DIVA were ambiguous for nodes roughly older than 80 Ma. More recent events were firmly assessed and involved post-Gondwanan long-distance dispersal. The Neotropics was colonized three times, all from the Afrotropical region, with the latest event less than 25 Ma that included the ancestor of all Neotropical Platypodini. Copyright © 2015 Elsevier Inc. All rights reserved.

    3. Genetic and antigenic analysis of H5N1 viruses for selection of HA-donor virus for vaccine strains.

      Science.gov (United States)

      Bhatia, S; Kunal, A; Khandia, R; Siddiqui, A; Pateriya, A K; Sood, R

      2013-12-01

      Genetic and antigenic analysis of H5N1 viruses, isolated in India during a period from year 2006 to 2010, was carried out for selection of the potential H5-HA (haemagglutinin) gene donor virus for developing a reverse genetics based DIVA marker H5 vaccine for poultry in India. Out of the 47 H5N1 viruses (clade 2.2), 14 representative viruses were selected on the basis of amino acid sequence analysis of HA1 gene for further antigenic characterization. Using antigenic cartography, an antigenic map was constructed based on the data of cross-HI (haemagglutinin inhibition) titration of 14 sera versus 14 viruses to visualize the relatedness among the antigens and antigenic coverage of the sera. Sera against five H5N1 viruses (A/crow/Assam/142119/2008, A/chicken/West Bengal/100879/2008, A/chicken/West Bengal/155505/2009, A/chicken/West Bengal/80995/2008 and A/chicken/West Bengal/81760/2008) exhibited maximum (100 %) antigenic coverage, hence, were selected as the potential HA donor viruses. However, the virus strain A/chicken/West Bengal/80995/2008 matched completely with the consensus amino acid sequence of the 47 viruses, therefore, was considered the best HA donor candidate out of the five showing 100 % antigenic coverage. The present study demonstrates a stepwise methodology for logical selection of vaccine strain or HA gene donor strain for developing H5 vaccines using genetic and antigenic data.

    4. Probabilistic historical biogeography: new models for founder-event speciation, imperfect detection, and fossils allow improved accuracy and model-testing

      Directory of Open Access Journals (Sweden)

      Nicholas Joseph Matzke

      2013-12-01

      Full Text Available Historical biogeography has been characterized by a large diversity of methods and unresolved debates about which processes, such as dispersal or vicariance, are most important for explaining distributions. A new R package, BioGeoBEARS, implements many models in a common likelihood framework, so that standard statistical model selection procedures can be applied to let the data choose the best model. Available models include a likelihood version of DIVA (“DIVALIKE”, LAGRANGE’s DEC model, and BAYAREA, as well as “+J” versions of these models which include founder-event speciation, an important process left out of most inference methods. I use BioGeoBEARS on a large sample of island and non-island clades (including two fossil clades to show that founder-event speciation is a crucial process in almost every clade, and that most published datasets reject the non-J models currently in widespread use. BioGeoBEARS is open-source and freely available for installation at the Comprehensive R Archive Network at http://CRAN.R-project.org/package=BioGeoBEARS. A step-by-step tutorial is available at http://phylo.wikidot.com/biogeobears.

    5. Ibandronate dose response is associated with increases in bone mineral density and reductions in clinical fractures: results of a meta-analysis.

      Science.gov (United States)

      Sebba, Anthony I; Emkey, Ronald D; Kohles, Joseph D; Sambrook, Philip N

      2009-03-01

      This meta-analysis pooled data from the four phase III clinical trials of ibandronate to assess the relationship between ibandronate dose, changes in bone mineral density, and rates of both clinical and non-vertebral fractures. Individual patient data from the intent-to-treat population of the BONE, IV fracture prevention, MOBILE, and DIVA studies were included for analysis. The relationship between ibandronate dose and bone mineral density at both the lumbar spine and at the total hip was assessed qualitatively. The relationship between lumbar spine bone mineral density and clinical fracture rate, and the relationship between total hip bone mineral density and non-vertebral fracture rate, were assessed both qualitatively and using mathematical models. A total of 8710 patients were included in this analysis. Both lumbar spine and total hip bone mineral density were observed to increase with increasing ibandronate dose. The incidence of all clinical fractures was observed to decrease as lumbar spine bone mineral density increased. A statistically significant inverse linear relationship was observed between percent change in lumbar spine bone mineral density and the rate of clinical fractures (P=0.005). A non-significant curvilinear relationship was observed between percent change in total hip bone mineral density and non-vertebral fracture rate. Increased ibandronate exposure is associated with increasing gains in the lumbar spine bone mineral density and decreasing clinical fracture rates. A non-linear relationship may exist between increases in the total hip bone mineral density and non-vertebral fracture rate.

    6. Development of a Façade Assessment and Design Tool for Solar Energy (FASSADES

      Directory of Open Access Journals (Sweden)

      Jouri Kanters

      2014-03-01

      Full Text Available Planning energy-efficient buildings which produce on-site renewable energy in an urban context is a challenge for all involved actors in the planning process. The primary objective of this study was to develop a façade assessment and design tool for solar energy (FASSADES providing the necessary information for all stakeholders in the design process. The secondary objective was to demonstrate the tool by performing an assessment analysis of a building block. The FASSADES tool is a DIVA4Rhino script, combining Radiance/Daysim and EnergyPlus for simulating the annual production of solar thermal and photovoltaic systems on facades, the cost-effectiveness of the solar energy system, and the payback time. Different output methods are available; graphically within the 3D drawing environment and numerically within post-processing software. The tool was tested to analyse a building block within a city under Swedish conditions. Output of the developed tool showed that shading from nearby buildings greatly affects the feasibility of photovoltaic and solar thermal systems on facades.

    7. Statistical methods of automatic spectral classification and their application to the Hamburg/ESO Survey

      CERN Document Server

      Christlieb, N; Grasshoff, G; Christlieb, Norbert; Wisotzki, Lutz; Grasshoff, Gerd

      2002-01-01

      We employ classical statistical methods of multivariate classification for the exploitation of the stellar content of the Hamburg/ESO objective prism survey (HES). In a simulation study we investigate the precision of a three-dimensional classification (Teff, log g, [Fe/H]) achievable in the HES for stars in the effective temperature range 520010 (typically corresponding to B_J<16.5). The accuracies in log g and [Fe/H] are better than 0.68dex in the same S/N range. These precisions allow for a very efficient selection of metal-poor stars in the HES. We present a minimum cost rule for compilation of complete samples of objects of a given class, and a rejection rule for identification of corrupted or peculiar spectra. The algorithms we present are being used for the identification of other interesting objects in the HES data base as well, and they are applicable to other existing and future large data sets, such as those to be compiled by the DIVA and GAIA missions.

    8. A new global interior ocean mapped climatology: the 1° × 1° GLODAP version 2

      Science.gov (United States)

      Lauvset, Siv K.; Key, Robert M.; Olsen, Are; van Heuven, Steven; Velo, Anton; Lin, Xiaohua; Schirnick, Carsten; Kozyr, Alex; Tanhua, Toste; Hoppema, Mario; Jutterström, Sara; Steinfeldt, Reiner; Jeansson, Emil; Ishii, Masao; Perez, Fiz F.; Suzuki, Toru; Watelet, Sylvain

      2016-08-01

      We present a mapped climatology (GLODAPv2.2016b) of ocean biogeochemical variables based on the new GLODAP version 2 data product (Olsen et al., 2016; Key et al., 2015), which covers all ocean basins over the years 1972 to 2013. The quality-controlled and internally consistent GLODAPv2 was used to create global 1° × 1° mapped climatologies of salinity, temperature, oxygen, nitrate, phosphate, silicate, total dissolved inorganic carbon (TCO2), total alkalinity (TAlk), pH, and CaCO3 saturation states using the Data-Interpolating Variational Analysis (DIVA) mapping method. Improving on maps based on an earlier but similar dataset, GLODAPv1.1, this climatology also covers the Arctic Ocean. Climatologies were created for 33 standard depth surfaces. The conceivably confounding temporal trends in TCO2 and pH due to anthropogenic influence were removed prior to mapping by normalizing these data to the year 2002 using first-order calculations of anthropogenic carbon accumulation rates. We additionally provide maps of accumulated anthropogenic carbon in the year 2002 and of preindustrial TCO2. For all parameters, all data from the full 1972-2013 period were used, including data that did not receive full secondary quality control. The GLODAPv2.2016b global 1° × 1° mapped climatologies, including error fields and ancillary information, are available at the GLODAPv2 web page at the Carbon Dioxide Information Analysis Center (CDIAC; doi:10.3334/CDIAC/OTG.NDP093_GLODAPv2).

    9. Tackling the issue of environmental survival of live Salmonella Typhimurium vaccines: deletion of the lon gene.

      Science.gov (United States)

      Leyman, Bregje; Boyen, Filip; Van Parys, Alexander; Verbrugghe, Elin; Haesebrouck, Freddy; Pasmans, Frank

      2012-12-01

      Vaccination is an important measure to control Salmonella contamination in the meat production chain. A previous study showed that both the ΔrfaJ and ΔrfaL strains are suitable markers and allow serological differentiation of infected and vaccinated animals. The aim of this study was to verify whether deletion of the lon gene in a Salmonella Typhimurium ΔrfaJ marker strain resulted in decreased environmental survival. Our results indicate that deletion of the lon gene in the ΔrfaJ strain did not affect invasiveness in IPEC-J2 cells and resulted in an increased susceptibility to UV, disinfectants (such as hydrogen peroxide and tosylchloramide sodium) and citric acid. Immunization of pigs with inactivated ΔrfaJ or ΔlonΔrfaJ vaccines allowed differentiation of infected and vaccinated pigs. Furthermore, deletion of the lon gene did not reduce the protection conferred by live wild type or ΔrfaJ vaccines against subsequent challenge with a virulent Salmonella Typhimurium strain in BALB/c mice. Based on our results in mice, we conclude that deletion of lon in ΔrfaJ contributes to environmental safety of the ΔrfaJ DIVA strain.

    10. Golden Jubilee Event for Cinema Fans

      CERN Multimedia

      2004-01-01

      As part of the Organisation's Fiftieth Anniversary celebrations the CERN CineClub will be showing a special series of films beginning with works by British, French, German, Norwegian and Spanish directors. The Club hopes to include films from all of the Member States in the programme. CERN's linguistic and cultural diversity will be celebrated from mid-February onwards when the CERN CineClub, whose summer English language film season will be familiar to many of you, plans to show a series of films from CERN's 20 Member States. The programme marks the CineClub's contribution to CERN's fiftieth anniversary festivities. The first film, "Four weddings and a funeral" (Mike Newell, 1994), will be shown in the Main Auditorium on 12 February. Next in the series will be films from Germany (Himmel über Berlin, or "The Wings of Desire", by Wim Wenders, 1987), Spain (Los Lunes al Sol, or "Mondays in the Sun", by Fernando Leon de Aranoa, 2002), France ( Diva , by Jean Jacques Beinex, 1981) and Norway (Elling, b...

    11. Evolution of flatworm central nervous systems: Insights from polyclads

      Directory of Open Access Journals (Sweden)

      Sigmer Y. Quiroga

      2015-09-01

      Full Text Available The nervous systems of flatworms have diversified extensively as a consequence of the broad range of adaptations in the group. Here we examined the central nervous system (CNS of 12 species of polyclad flatworms belonging to 11 different families by morphological and histological studies. These comparisons revealed that the overall organization and architecture of polyclad central nervous systems can be classified into three categories (I, II, and III based on the presence of globuli cell masses -ganglion cells of granular appearance-, the cross-sectional shape of the main nerve cords, and the tissue type surrounding the nerve cords. In addition, four different cell types were identified in polyclad brains based on location and size. We also characterize the serotonergic and FMRFamidergic nervous systems in the cotylean Boninia divae by immunocytochemistry. Although both neurotransmitters were broadly expressed, expression of serotonin was particularly strong in the sucker, whereas FMRFamide was particularly strong in the pharynx. Finally, we test some of the major hypothesized trends during the evolution of the CNS in the phylum by a character state reconstruction based on current understanding of the nervous system across different species of Platyhelminthes and on up-to-date molecular phylogenies.

    12. CanCan zīmola komunikācija un klientu lojalitāte

      OpenAIRE

      Antuseviča, Nadežda

      2016-01-01

      Bakalaura darba tēma ir “CanCan zīmola komunikācija un klientu lojalitāte”. Bakalaura darba mērķis ir noskaidrot kādus “CanCan” zīmols izmanto komunikācijas instrumentus un aktivitātes, un kā tie ietekmē klientu lojalitāti. Darbs sastāv no sekojošām daļām: ievads, teorētiskā, metodoloģijas, empīriskā daļa, secinājumi un pielikums. Teorētisko daļu veido divas galvenās nodaļas: zīmols un klientu lojalitāte. Apakšnodaļās ir aprakstīti zīmola klasifikācija, zīmola tēls, zīmola pozicionēšana, zīmo...

    13. Anticipation in stuttering: A theoretical model of the nature of stutter prediction.

      Science.gov (United States)

      Garcia-Barrera, Mauricio A; Davidow, Jason H

      2015-06-01

      The fact that some people who stutter have the ability to anticipate a stuttering moment is essential for several theories of stuttering and important for maximum effectiveness of many currently used treatment techniques. The "anticipation effect," however, is poorly understood despite much investigation into this phenomenon. In the present paper, we combine (1) behavioral evidence from the stuttering-anticipation literature, (2) speech production models, and (3) models of error detection to propose a theoretical model of anticipation. Integrating evidence from theories such as Damasio's Somatic Marker Hypothesis, Levelt's Perceptual Monitoring Theory, Guenther's The Directions Into Velocities of Articulators (DIVA) model, Postma's Covert Repair Hypothesis, among others, our central thesis is that the anticipation of a stuttering moment occurs as an outcome of the interactions between previous learning experiences (i.e., learnt associations between stuttered utterances and any self-experienced or environmental consequence) and error monitoring. Possible neurological mechanisms involved in generating conscious anticipation are also discussed, along with directions for future research. The reader will be able to: (a) describe historical theories that explain how PWS may learn to anticipate stuttering; (b) state some traditional sources of evidence of anticipation in stuttering; (c) describe how PWS may be sensitive to the detection of a stuttering; (d) state some of the neural correlates that may underlie anticipation in stuttering; and (e) describe some of the possible utilities of incorporating anticipation into stuttering interventions. Copyright © 2015 Elsevier Inc. All rights reserved.

    14. Morphology-based phylogeny and biogeography of Wockia (Lepidoptera: Urodidae) with description of a new species from Japan and South Korea.

      Science.gov (United States)

      Sohn, Jae-Cheon

      2014-04-01

      Wockia, one of the six genera within the lepidopteran family Urodidae, currently includes nine species distributed in Holarctic, Oriental, and Neotropical regions. A new species of the genus, W. magna sp. nov., is described from Japan and South Korea. This is the first record of Urodidae from Japan. A cladistic analysis was constructed based on 27 morphological characters from five ingroup species and two outgroup taxa. A single most parsimonious tree was found (length = 38, CI = 71, RI = 70). The resulting tree failed to recover the monophyly of Wockia chewbacca and a clade Wockia sensu stricto, including four congeners from North America, temperate East Asia and Southeast Asia. Wockia sensu stricto was resolved as monophyletic and was divided into two subgroups, one including W. asperipunctella and W. magna, and the other including W. koreana and W. variata. The sister-group relationship of W. asperipunctella and W. magna was moderately supported. Synapomorphies of Wockia sensu stricto are provided from the resulting phylogeny. Systematic definitions of Wockia and other allied genera are revised. Optimal ancestral area reconstruction implemented in DIVA resulted in different hypotheses for Wockia, depending on how to constrain the maximum number of areas. The maximum area number set as two yielded a more likely scenario suggesting that Wockia sensu stricto originated in temperate East Asia and then dispersed into the Oriental region, North America, and Europe. A working hypothesis and other equally possible alternative explanations for the biogeography of Wockia are provided.

    15. Self-assembly and release of peste des petits ruminants virus-like particles in an insect cell-baculovirus system and their immunogenicity in mice and goats.

      Directory of Open Access Journals (Sweden)

      Wenchao Li

      Full Text Available Peste des petits ruminants (PPR is an acute, febrile, viral disease of small ruminants that has a significant economic impact. For many viral diseases, vaccination with virus-like particles (VLPs has shown considerable promise as a prophylactic approach; however, the processes of assembly and release of peste des petits ruminants virus (PPRV VLPs are not well characterized, and their immunogenicity in the host is unknown. In this study, VLPs of PPRV were generated in a baculovirus system through simultaneous expression of PPRV matrix (M protein and hemaglutin in (H or fusion (F protein. The released VLPs showed morphology similar to that of the native virus particles. Subcutaneous injection of these VLPs (PPRV-H, PPRV-F into mice and goats elicited PPRV-specific IgG production, increased the levels of virus neutralizing antibodies, and promoted lymphocyte proliferation. Without adjuvants, the immune response induced by the PPRV-H VLPs was comparable to that obtained using equivalent amounts of PPRV vaccine. Thus, our results demonstrated that VLPs containing PPRV M protein and H or F protein are potential "differentiating infected from vaccinated animals" (DIVA vaccine candidates for the surveillance and eradication of PPR.

    16. High-resolution melting (HRM) for genotyping bovine ephemeral fever virus (BEFV).

      Science.gov (United States)

      Erster, Oran; Stram, Rotem; Menasherow, Shopia; Rubistein-Giuni, Marisol; Sharir, Binyamin; Kchinich, Evgeni; Stram, Yehuda

      2017-02-02

      In recent years there have been several major outbreaks of bovine ephemeral disease in the Middle East, including Israel. Such occurrences raise the need for quick identification of the viruses responsible for the outbreaks, in order to rapidly identify the entry of viruses that do not belong to the Middle-East BEFV lineage. This challenge was met by the development of a high-resolution melt (HRM) assay. The assay is based on the viral G gene sequence and generation of an algorithm that calculates and evaluates the GC content of various fragments. The algorithm was designed to scan 50- to 200-base-long segments in a sliding-window manner, compare and rank them using an Order of Technique of Preference by Similarity to Ideal Solution (TOPSIS) the technique for order preference by similarity to ideal solution technique, according to the differences in GC content of homologous fragments. Two fragments were selected, based on a match to the analysis criteria, in terms of size and GC content. These fragments were successfully used in the analysis to differentiate between different virus lineages, thus facilitating assignment of the viruses' geographical origins. Moreover, the assay could be used for differentiating infected from vaccinated animales (DIVA). The new algorithm may therefore be useful for development of improved genotyping studies for other viruses and possibly other microorganisms.

    17. Vēstījuma atstatuma mazināšana video spēlēs

      OpenAIRE

      Husko, Rihards

      2012-01-01

      Pētījums Vēstījuma atstatuma mazināšana video spēlēs tapis ar nolūku apskatīt rīkus, ar kuru palīdzību iespējams palielināt spēlētāju emocionālo ieguldījumu datorspēles pasaulē. Tas panākts aplūkojot dažādas ar vēstījumu spēlēs saistītas teorijas un analizējot izvēlētu video spēļu vēstījumus. Kā pētījuma mērķis tika izvēlētas pirmās divas Mass Effect triloģijas spēles, ir atsauces arī uz citām spēlēm salīdzināšanas nolūkos. Teorētiskā pētījuma daļa atklāja, ka spēles līdz šim nav tikušas anal...

    18. Multiple origins of Heliozoa from flagellate ancestors: New cryptist subphylum Corbihelia, superclass Corbistoma, and monophyly of Haptista, Cryptista, Hacrobia and Chromista.

      Science.gov (United States)

      Cavalier-Smith, Thomas; Chao, Ema E; Lewis, Rhodri

      2015-12-01

      Heliozoan protists have radiating cell projections (axopodia) supported by microtubular axonemes nucleated by the centrosome and bearing granule-like extrusomes for catching prey. To clarify previously confused heliozoan phylogeny we sequenced partial transcriptomes of two tiny naked heliozoa, the endohelean Microheliella maris and centrohelid Oxnerella marina, and the cercozoan pseudoheliozoan Minimassisteria diva. Phylogenetic analysis of 187 genes confirms that all are chromists; but centrohelids (microtubules arranged as hexagons and triangles) are not sisters to Endohelea having axonemes in transnuclear cytoplasmic channels (triangular or square microtubular arrays). Centrohelids are strongly sister to haptophytes (together phylum Haptista); we explain the common origins of their axopodia and haptonema. Microheliella is sister to new superclass Corbistoma (zooflagellate Telonemea and Picomonadea, with asymmetric microfilamentous pharyngeal basket), showing that these axopodial protists evolved independently from zooflagellate ancestors. We group Corbistoma and Endohelea as new cryptist subphylum Corbihelia with dense fibrillar interorganellar connections; endohelean axopodia and Telonema cortex are ultrastructurally related. Differently sampled trees clarify why corticate multigene eukaryote phylogeny is problematic: long-branch artefacts probably distort deep multigene phylogeny of corticates (Plantae, Chromista); basal radiations may be contradictorily reconstructed because of their extreme closeness and the Bayesian star-tree paradox. Haptista and Hacrobia are holophyletic, and Chromista probably are.

    19. Novel cultured protists identify deep-branching environmental DNA clades of cercozoa: New Genera Tremula, Micrometopion, Minimassisteria, Nudifila, Peregrinia.

      Science.gov (United States)

      Howe, Alexis T; Bass, David; Scoble, Josephine M; Lewis, Rhodri; Vickerman, Keith; Arndt, Hartmut; Cavalier-Smith, Thomas

      2011-04-01

      We describe three new orders of filosan Cercozoa, five new deep-branching genera, eight new species of Thaumatomonas, Reckertia, Spongomonas, Rhogostoma, Agitata, Neoheteromita and Paracercomonas, sequence their 18S rDNA, and construct 18S rDNA trees for 148 Cercozoa. Our phylogeny indicates that Filosa were ancestrally gliding flagellates; non-flagellate filose amoebae evolved from them five times independently. The new genera are more closely related to environmental DNA sequences than cultured organisms. Tremula longifila, a zooflagellate glider on both flagella (unlike other Cercozoa), is the most divergent filosan (Tremulida ord. n.). Micrometopion nutans is a eukaryote-eating gliding zooflagellate like Metopion and Metromonas. Minimassisteria diva is a widespread trimorphic marine amoeboflagellate granofilosan. Peregrinia clavideferens, a non-testate, scale-bearing, filose amoeba, branches deeply in Thaumatomonadida, which are probably sisters to Spongomonadida. Nudifila producta is a filose amoeboflagellate related to Clautriavia and Marimonadida (ord. n., e.g. Pseudopirsonia, Auranticordis). We substantially revise Imbricatea, now including Spongomonadida, and Thecofilosea to include Phaeodaria. Thecofilosea and Imbricatea and Thecofilosea are sisters, both arguably ancestrally rigid gliding flagellates with ventral pseudopod-emitting grooves. Scale-free Ovulinata parva is sister to Paulinella, so imbricate silica scales can be lost. Internal hollow silica skeletons evolved twice in Thecofilosea (Ebriida, Phaeodaria) or were multiply lost. Protaspa replaces preoccupied 'Protaspis'.

    20. Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.

      Science.gov (United States)

      Tchasovnikarova, Iva A; Timms, Richard T; Douse, Christopher H; Roberts, Rhys C; Dougan, Gordon; Kingston, Robert E; Modis, Yorgo; Lehner, Paul J

      2017-07-01

      Dominant mutations in the MORC2 gene have recently been shown to cause axonal Charcot-Marie-Tooth (CMT) disease, but the cellular function of MORC2 is poorly understood. Here, through a genome-wide CRISPR-Cas9-mediated forward genetic screen, we identified MORC2 as an essential gene required for epigenetic silencing by the HUSH complex. HUSH recruits MORC2 to target sites in heterochromatin. We exploited a new method, differential viral accessibility (DIVA), to show that loss of MORC2 results in chromatin decompaction at these target loci, which is concomitant with a loss of H3K9me3 deposition and transcriptional derepression. The ATPase activity of MORC2 is critical for HUSH-mediated silencing, and the most common alteration affecting the ATPase domain in CMT patients (p.Arg252Trp) hyperactivates HUSH-mediated repression in neuronal cells. These data define a critical role for MORC2 in epigenetic silencing by the HUSH complex and provide a mechanistic basis underpinning the role of MORC2 mutations in CMT disease.

    1. ANÁLISIS FILOGENÉTICO Y BIOGEOGRÁFICO DE LAS ESPECIES DEL GÉNERO BRYCONAMERICUS (CHARACIFORMES, CHARACIDAE DE LA BAJA AMÉRICA CENTRAL

      Directory of Open Access Journals (Sweden)

      Román Cesar

      2009-12-01

      Full Text Available Se presenta una hipótesis preliminar de monofi lia para las especies de Bryconamericusde la baja América Central, basada en caracteres moleculares de ADNmt, que resultaen el siguiente arreglo de relaciones fi logenéticas: (B. emperador (B. gonzalezoi(B. bayano, (B. terrabensis, B. scleroparius. Esta hipótesis es preliminar en elcontexto fi logenético del género, sin embargo, aumenta la probabilidad de que estegrupo se constituya en un grupo natural. Se halla concordancia entre la hipótesisde relaciones presentada para las especies de Bryconamericus analizadas aquí y loshallazgos con base en caracteres morfológicos. A través de biogeografi a cladística,cladogramas de áreas, cladogramas resueltos de áreas y Análisis de Dispersión-Vicarianza (DIVA se comprueba que la hipótesis biogeográfi ca para las especiesde Bryconamericus está relacionada con un proceso de dispersión desde el noroestede América del Sur, que permitió colonizar la baja Centroamérica. Simultáneamenteocurrieron extinciones, y como producto de la reorganización geológica y aislamientogeográfi co de las poblaciones, aparece la vicarianza.

    2. Evolution of flatworm central nervous systems: Insights from polyclads

      Science.gov (United States)

      Quiroga, Sigmer Y.; Carolina Bonilla, E.; Marcela Bolaños, D.; Carbayo, Fernando; Litvaitis, Marian K.; Brown, Federico D.

      2015-01-01

      The nervous systems of flatworms have diversified extensively as a consequence of the broad range of adaptations in the group. Here we examined the central nervous system (CNS) of 12 species of polyclad flatworms belonging to 11 different families by morphological and histological studies. These comparisons revealed that the overall organization and architecture of polyclad central nervous systems can be classified into three categories (I, II, and III) based on the presence of globuli cell masses -ganglion cells of granular appearance-, the cross-sectional shape of the main nerve cords, and the tissue type surrounding the nerve cords. In addition, four different cell types were identified in polyclad brains based on location and size. We also characterize the serotonergic and FMRFamidergic nervous systems in the cotylean Boninia divae by immunocytochemistry. Although both neurotransmitters were broadly expressed, expression of serotonin was particularly strong in the sucker, whereas FMRFamide was particularly strong in the pharynx. Finally, we test some of the major hypothesized trends during the evolution of the CNS in the phylum by a character state reconstruction based on current understanding of the nervous system across different species of Platyhelminthes and on up-to-date molecular phylogenies. PMID:26500427

    3. Mājdzīvnieku (mājas – istabas dzīvnieku) pozicionēšana vegānismā

      OpenAIRE

      Dziedātāja, Elīna

      2015-01-01

      Bakalaura darbā “Mājdzīvnieku pozicionēšana vegānismā” tiek noskaidrots kā sadzīvo divas, savā starpā šķietami pretrunīgas prakses – vegānisms, kas ir kustība, kuras dalībnieki uzskata par morālu pienākumu respektēt dzīvnieku intereses, ar mājdzīvnieku jeb istabas – mājas dzīvnieku turēšanu. Vegānisms tiek skatīts kā viena no veģetārisma apakšgrupām, tomēr netiek saistīts tikai un vienīgi ar uzturu, bet lielākoties balstās uz ētisku izturēšanos pret dzīvniekiem. P...

    4. The spatial and temporal distribution of microalgae in the South China Sea: evidence from GIS-based analysis of 18S rDNA sequences

      Institute of Scientific and Technical Information of China (English)

      LI LvYan; HUANG QiaoJuan; WU ShuHui; LIN Duan; CHEN JiaHui; CHEN YueQin

      2008-01-01

      The purpose of this study was to estimate the spatial and temporal variation of microalgae in the South China Sea and to demonstrate the environmental factors controlling the diversity of microalgae by GIS (geographic information system)-based analysis of 18S rDNA sequences. Six 18S rDNA libraries were constructed from environmental samples collected at different sites in the study area, and more than 600 18S rDNA sequences were determined. The rDNA sequence data were then analyzed by DIVA-GIS software to display the spatial and temporal variation of phytoplankton's composition. It was shown that the autotrophic eukaryotic plankton dominated over the heterotrophic cells in most of our clone libraries, and the dominating phytoplankton was Dinophyceae except for Bacillariophyta at the Xiamen harbor. The percentages of these two groups were controlled by water temperature and salinity. Our results also revealed that the species composition of Chlorophyta showed a close relationship with latitude, changing from Prasinophyceae at the high latitude to Trebouxiophyceae at the low latitude. Several newly classified picoplankton lineages were first uncovered in the South China Sea, including the pico-sized green alga Ostreococcus sp. and Picochlorum eukaryotum, and picobiliphytes, which was just discovered in 2007 with unknown affinities to other eukaryotes. Their spatial and temporal variation were also analyzed and discussed.

    5. Predicting the potential geographic distribution of cotton mealybug Phenacoccus solenopsis in India based on MAXENT ecological niche model.

      Science.gov (United States)

      Fand, Babasaheb B; Kumar, Mahesh; Kamble, Ankush L

      2014-09-01

      Mealybug, Phenacoccus solenopsis Tinsley has recently emerged as a serious insect pest of cotton in India. This study demonstrates the use of Maxent algorithm for modeling the potential geographic distribution of P. solenopsis in India with presence-only data. Predictions were made based on the analysis of the relationship between 111 occurrence records for P. solenopsis and the corresponding current and future climate data defined on the study area. The climate data from worldclim database for current (1950-2000) and future (SRES A2 emission scenario for 2050) conditions were used. DIVA-GIS, an open source software for conducting spatial analysis was used for mapping the predictions from Maxent. The algorithm provided reasonable estimates of the species range indicating better discrimination of suitable and unsuitable areas for its occurrence in India under both present and future climatic conditions. The fit for the model as measured by AUC was high, with value of 0.930 for the training data and 0.895 for the test data, indicating the high level of discriminatory power for the Maxent. A Jackknife test for variable importance indicated that mean temperature of coldest quarter with highest gain value was the most important environmental variable determining the potential geographic distribution of P. solenopsis. The approaches used for delineating the ecological niche and prediction of potential geographic distribution are described briefly. Possible applications and limitations of the present modeling approach in future research and as a decision making tool in integrated pest management are discussed.

    6. The Solar Tower at Monte Mario: a New Didactic Laboratory for Astronomy

      Science.gov (United States)

      D'Alessio, F.; Faccini, M.; Leoni, R.; Giobbi, G.

      The year 2009 witnessed the restoration of the Solar Tower of Astronomical Observatory of Rome, in the INAF main office of Villa Mellini, Monte Mario. This structure underwent a patient restructuring of optics, mechanics and control equipment. Moreover, some areas have been converted into welcome centre for the visitors, so as to transform a scientific instrument - which was active up to a few years ago - into a didactic structure, mainly suitable for school groups. The Solar Tower of Monte Mario, which has been open to the public since January 2010, allows visitors to directly observe the Sun, as well as the spectrum of its light. The Tower therefore becomes an effective didactic instrument in order to teach and talk about a wide range of scientific topics - from Solar Physics to Sun-Earth interactions, to Stellar Evolution. Here we describe the work done by the DivA group of INAF-OAR for putting back into service the Solar Tower, from the technical and didactic viewpoints. We shall also take stock of the activities organized in the first few months of activity.

    7. Estimating Ancestral Ranges: Testing Methods with a Clade of Neotropical Lizards (Iguania: Liolaemidae)

      Science.gov (United States)

      Díaz Gómez, Juan Manuel

      2011-01-01

      Establishing the ancestral ranges of distribution of a monophyletic clade, called the ancestral area, is one of the central objectives of historical biogeography. In this study, I used three common methodologies to establish the ancestral area of an important clade of Neotropical lizards, the family Liolaemidae. The methods used were: Fitch optimization, Weighted Ancestral Area Analysis and Dispersal-Vicariance Analysis (DIVA). A main difference from previous studies is that the areas used in the analysis are defined based on actual distributions of the species of Liolaemidae, instead of areas defined arbitrarilyor based on other taxa. The ancestral area of Liolaemidae found by Fitch optimization is Prepuna on Argentina, Central Chile and Coastal Peru. Weighted Ancestral Area Analysis found Central Chile, Coquimbo, Payunia, Austral Patagonia and Coastal Peru. Dispersal-Vicariance analysis found an ancestral area that includes almost all the areas occupied by Liolaemidae, except Atacama, Coquimbo and Austral Patagonia. The results can be resumed on two opposing hypothesis: a restricted ancestral area for the ancestor of Liolaemidae in Central Chile and Patagonia, or a widespread ancestor distributed along the Andes. Some limitations of the methods were identified, for example the excessive importance of plesiomorphic areas in the cladograms. PMID:22028873

    8. A modeling investigation of articulatory variability and acoustic stability during American English /are/ production

      Science.gov (United States)

      Nieto-Castanon, Alfonso; Guenther, Frank H.; Perkell, Joseph S.; Curtin, Hugh D.

      2005-05-01

      This paper investigates the functional relationship between articulatory variability and stability of acoustic cues during American English /are/ production. The analysis of articulatory movement data on seven subjects shows that the extent of intrasubject articulatory variability along any given articulatory direction is strongly and inversely related to a measure of acoustic stability (the extent of acoustic variation that displacing the articulators in this direction would produce). The presence and direction of this relationship is consistent with a speech motor control mechanism that uses a third formant frequency (F3) target; i.e., the final articulatory variability is lower for those articulatory directions most relevant to determining the F3 value. In contrast, no consistent relationship across speakers and phonetic contexts was found between hypothesized vocal-tract target variables and articulatory variability. Furthermore, simulations of two speakers' productions using the DIVA model of speech production, in conjunction with a novel speaker-specific vocal-tract model derived from magnetic resonance imaging data, mimic the observed range of articulatory gestures for each subject, while exhibiting the same articulatory/acoustic relations as those observed experimentally. Overall these results provide evidence for a common control scheme that utilizes an acoustic, rather than articulatory, target specification for American English /are/. .

    9. Mycetoma by Nocardia asteroides: a 9 year folow-up Micetoma por Nocardia asteroides: acompanhamento de 9 anos

      Directory of Open Access Journals (Sweden)

      Giani de Oliveira Saraça

      1993-04-01

      Full Text Available An extensive and severe actinomycetoma by Nocardia asteroides, a rare etiologic agent of this infection in Brazil, observed during a 9 year follow-up is reported. Unsuitable social and financial conditions led to amputation as the only possible solution for this case, no signs of infection relapse having been observed in three years after his surgery.É relatado um caso de micetoma actinomicótico por Nocardia asteroides, raro agente desta infecção no Brasil, acompanhado ao longo de 9 anos de evolução, com lesões extensas e profundas. As precárias condições sócio-econômicas do paciente tornaram a amputação a única solução viável para este caso, não tendo sido observados sinais de reci-diva da infecção em três anos após a cirurgia.

    10. MODEL TRANSFORMATION DEVELOPMENT USING MOLA MAPPINGS AND TEMPLATE MOLA

      OpenAIRE

      Kalniņa, Elīna

      2012-01-01

      Darbā pētīta modeļu transformāciju izstrāde 3 specializētos problēmu apgabalos: transformācijas modeļ-bāzētai izstrādei, transformācijas grafisku modelēšanas valodu rīku būvei un transformācijas, kas sintezē transformācijas. Secināts, ka valodā, kas pielāgota konkrētajam apgabalam, transformāciju izstrāde ir vienkāršāka un ātrāka. Visiem trim pētītajiem apgabaliem tika izstrādātas tiem pielāgotas valodas. Divas no izstrādātajām valodām balstās uz attēlojumu principiem. Abos gadījumos tika sec...

    11. Mycobacterium bovis: realities and challenges for the veterinary biopharmaceutical industry

      Directory of Open Access Journals (Sweden)

      Aníbal Domínguez Odio

      2016-01-01

      Full Text Available Mycobacterium bovis is the main etiological agent of bovine tuberculosis, bacterial diseases of world distribution, chronicle, of easy transmission, debilitating, zoonotic and antropozoonotic that affects any organ and which can be presented without symptoms On this base, it was carried out a study with the objective of approaching the current state and the scientific-technological projections for the prevention and diagnosis of the bovine tuberculosis, caused by M. bovis. It was demonstrated that the 45.09% of the original articles on inmunoprophylaxis against bacteria, registered in the Scopus database and contextualised until principles of 2014, were focused toward M. bovis. In spite of the advances in molecular biology and the hopes deposited in the Ag85A, Rv0287, Rv0288, Rv0251c, MPB70, MPB83, ESAT-6 and CFP-10 molecules, jointly with their combinations, it will continue absent in the market an effective, safety and differentiating vaccine; as well as a robust DIVA diagnosis system. It can be concluded that in the next 5 years, an officially recognized vacinal formulation will continue absent and that the tuberculin test in spite of its weaknesses will continue being the main tool of surveillance.

    12. Identifying Areas for Field Conservation of Forages in Latin American Disturbed Environments

      Directory of Open Access Journals (Sweden)

      Michael Peters

      2005-06-01

      Full Text Available This paper uses the spatial analysis tools DIVA and FloraMap to identify potential areas for the in situ conservation of a set of 10 forage species. We introduce the idea of roadside verges as conservation areas and discuss the risks and opportunities of two potential scenarios for conservation. These are the introduction of mass reservoirs outside of the original areas of collection and conservation inside the area of origin. Four potential areas for in situ conservation in Latin America are identified. Although more detailed studies using remote sensing, soil information, and field reconnaissance will be necessary for a final assessment of the suggested areas as field conservation sites, we discuss the possibilities of establishing low-maintenance communities and the potential dangers of introducing harmful weed species. We do not have final answers with regard to the permanent maintenance of genetic diversity in these areas but suggest that further studies of genetic drift in the populations would not only be scientifically useful but might also lead to identifying useful genotypes for local use.

    13. An Ancient Divide in a Contiguous Rainforest: Endemic Earthworms in the Australian Wet Tropics.

      Directory of Open Access Journals (Sweden)

      Corrie S Moreau

      Full Text Available Understanding the factors that shape current species diversity is a fundamental aim of ecology and evolutionary biology. The Australian Wet Tropics (AWT are a system in which much is known about how the rainforests and the rainforest-dependent organisms reacted to late Pleistocene climate changes, but less is known about how events deeper in time shaped speciation and extinction in this highly endemic biota. We estimate the phylogeny of a species-rich endemic genus of earthworms (Terrisswalkerius from the region. Using DEC and DIVA historical biogeography methods we find a strong signal of vicariance among known biogeographical sub-regions across the whole phylogeny, congruent with the phylogeography of less diverse vertebrate groups. Absolute dating estimates, in conjunction with relative ages of major biogeographic disjunctions across Australia, indicate that diversification in Terrisswalkerius dates back before the mid-Miocene shift towards aridification, into the Paleogene era of isolation of mesothermal Gondwanan Australia. For the Queensland endemic Terrisswalkerius earthworms, the AWT have acted as both a museum of biological diversity and as the setting for continuing geographically structured diversification. These results suggest that past events affecting organismal diversification can be concordant across phylogeographic to phylogenetic levels and emphasize the value of multi-scale analysis, from intra- to interspecies, for understanding the broad-scale processes that have shaped geographic diversity.

    14. An Ancient Divide in a Contiguous Rainforest: Endemic Earthworms in the Australian Wet Tropics.

      Science.gov (United States)

      Moreau, Corrie S; Hugall, Andrew F; McDonald, Keith R; Jamieson, Barrie G M; Moritz, Craig

      2015-01-01

      Understanding the factors that shape current species diversity is a fundamental aim of ecology and evolutionary biology. The Australian Wet Tropics (AWT) are a system in which much is known about how the rainforests and the rainforest-dependent organisms reacted to late Pleistocene climate changes, but less is known about how events deeper in time shaped speciation and extinction in this highly endemic biota. We estimate the phylogeny of a species-rich endemic genus of earthworms (Terrisswalkerius) from the region. Using DEC and DIVA historical biogeography methods we find a strong signal of vicariance among known biogeographical sub-regions across the whole phylogeny, congruent with the phylogeography of less diverse vertebrate groups. Absolute dating estimates, in conjunction with relative ages of major biogeographic disjunctions across Australia, indicate that diversification in Terrisswalkerius dates back before the mid-Miocene shift towards aridification, into the Paleogene era of isolation of mesothermal Gondwanan Australia. For the Queensland endemic Terrisswalkerius earthworms, the AWT have acted as both a museum of biological diversity and as the setting for continuing geographically structured diversification. These results suggest that past events affecting organismal diversification can be concordant across phylogeographic to phylogenetic levels and emphasize the value of multi-scale analysis, from intra- to interspecies, for understanding the broad-scale processes that have shaped geographic diversity.

    15. “Las cosas singulares de piedras, animales, plantas”: La formación y el funcionamiento de la red imperial española de remesas centíficas en el Virreinato del Río de la Plata

      Directory of Open Access Journals (Sweden)

      Magnus Roberto de Mello Pereira

      2013-01-01

      Full Text Available El presente artículo acompaña el proceso de remesa de especímenes animales y vegetales de la región administrativa del Virreinato del Río de la Plata para las instituciones científicas españolas, tales como el Real Gabinete, el Real Jardín Botánico y las menageries del Retiro y de Aranjuez. El propósito fue intentar sorprender esas remesas en el exacto momento en que tales instituciones y sus redes de recolección estaban siendo creadas, buscando expli- car las lógicas que las presidían, en un momento en que se mezclaban propósitos de orden económico; la cultura de la dádiva, típica del Antiguo Régimen; con las modernas prácticas científicas que estaban en construcción.

    16. [Digital subtraction angiography with carbon dioxide in severe arterial ischemia and allergy to iodinated compounds].

      Science.gov (United States)

      Calvo Cascallo, J; Mundi Salvadó, N; Cardona Fontanet, M

      1993-01-01

      When in some selected patients, a direct arterial surgery (DAS) procedure or an endoluminal surgery (ES) are required for a chronic arterial ischemia (III or IV degrees), and an arteriography with contrast is absolutely contraindicated (because of severe renal failure without hemodialysis program or a severe congestive heart failure or a hyperthyroidism or a seriously demonstrated hypersensibility against the contrast agents); an angiography by digital subtraction with carbon dioxide (DIVAS-CO2) is indicated. This technique provides good quality images with minimal risks for the patient and an adequate study for ulterior treatment. We report a case of a 67-years-old woman, with diabetes-II, ischemic cardiopathy, arterial hypertension and a demonstrated hypersensibility against the iodide compounds. The patient was admitted because of a chronic ischemia (IV degree) with ischemic ulcerations on some fingers from the left foot. High doses of analgesic drugs were needed. Because the hypersensibility against the iodide compounds, an angiography with CO2 was carried out. The good quality images provided by this technique showed the factibility of a revascularization.

    17. Biogeography and speciation patterns of the golden orb spider genus Nephila (Araneae: Nephilidae) in Asia.

      Science.gov (United States)

      Su, Yong-Chao; Chang, Yung-Hau; Smith, Deborah; Zhu, Ming-Sheng; Kuntner, Matjaž; Tso, I-Min

      2011-01-01

      The molecular phylogeny of the globally distributed golden orb spider genus Nephila (Nephilidae) was reconstructed to infer its speciation history, with a focus on SE Asian/W Pacific species. Five Asian, two Australian, four African, and one American species were included in the phylogenetic analyses. Other species in Nephilidae, Araneidae, and Tetragnathidae were included to assess their relationships with the genus Nephila, and one species from Uloboridae was used as the outgroup. Phylogenetic trees were reconstructed from one nuclear (18S) and two mitochondrial (COI and 16S) markers. Our molecular phylogeny shows that the widely distributed Asian/Australian species, N. pilipes, and an African species, N. constricta, form a clade that is sister to all other Nephila species. Nested in this Nephila clade are one clade with tropical and subtropical/temperate Asian/Australian species, and the other containing African and American species. The estimated divergence times suggest that diversification events within Nephila occurred during mid-Miocene to Pliocene (16 Mya-2 Mya), and these time periods were characterized by cyclic global warming/cooling events. According to Dispersal and Vicariance Analysis (DIVA), the ancestral range of the Asian/Australian clade was tropical Asia, and the ancestral range of the genus Nephila was either tropical Asia or Africa. We conclude that the speciation of the Asian/Australian Nephila species was driven by Neogene global cyclic climate changes. However, further population level studies comparing diversification patterns of sister species are needed to determine the mode of speciation of these species.

    18. Doctoral e-Theses; experiences in harvesting on a national and European level

      CERN Document Server

      CERN. Geneva

      2007-01-01

      In many countries in Europe doctoral e-theses are an integrated part of institutional repositories, which have been set up in recent years. In some countries they have been harvested on a national level, like in the Netherlands with the ‘Promise of Science’ portal (http://www.darenet.nl/promiseofscience) . In October 2006 SURFfoundation (The Netherlands), JISC (UK) and DIVA (funded through BIBSAM in Sweden) started a common project to harvest repositories with e-theses on an international scale and to set up a freely accessible European portal and test in practice the interoperability. The project, which aims at creating a value added service for doctoral e-Theses, will finish in June 2007. In the presentation we will show some lessons learned and the first results of the Demonstrator, an interoperable portal of European doctoral e-theses in five countries: Denmark, Germany, the Netherlands, Sweden and the UK. Furthermore, we will present the developments regarding the European coordination of doctoral...

    19. Daylight Design of Office Buildings: Optimisation of External Solar Shadings by Using Combined Simulation Methods

      Directory of Open Access Journals (Sweden)

      Javier González

      2015-05-01

      Full Text Available Integrating daylight and energy performance with optimization into the design process has always been a challenge for designers. Most of the building environmental performance simulation tools require a considerable amount of time and iterations for achieving accurate results. Moreover the combination of daylight and energy performances has always been an issue, as different software packages are needed to perform detailed calculations. A simplified method to overcome both issues using recent advances in software integration is explored here. As a case study; the optimization of external shadings in a typical office space in Australia is presented. Results are compared against common solutions adopted as industry standard practices. Visual comfort and energy efficiency are analysed in an integrated approach. The DIVA (Design, Iterate, Validate and Adapt plug-in for Rhinoceros/Grasshopper software is used as the main tool, given its ability to effectively calculate daylight metrics (using the Radiance/Daysim engine and energy consumption (using the EnergyPlus engine. The optimization process is carried out parametrically controlling the shadings’ geometries. Genetic Algorithms (GA embedded in the evolutionary solver Galapagos are adopted in order to achieve close to optimum results by controlling iteration parameters. The optimized result, in comparison with conventional design techniques, reveals significant enhancement of comfort levels and energy efficiency. Benefits and drawbacks of the proposed strategy are then discussed.

    20. Vaccination with recombinant adenoviruses expressing the peste des petits ruminants virus F or H proteins overcomes viral immunosuppression and induces protective immunity against PPRV challenge in sheep.

      Directory of Open Access Journals (Sweden)

      José M Rojas

      Full Text Available Peste des petits ruminants (PPR is a highly contagious disease of small ruminants caused by the Morbillivirus peste des petits ruminants virus (PPRV. Two recombinant replication-defective human adenoviruses serotype 5 (Ad5 expressing either the highly immunogenic fusion protein (F or hemagglutinin protein (H from PPRV were used to vaccinate sheep by intramuscular inoculation. Both recombinant adenovirus vaccines elicited PPRV-specific B- and T-cell responses. Thus, neutralizing antibodies were detected in sera from immunized sheep. In addition, we detected a significant antigen specific T-cell response in vaccinated sheep against two different PPRV strains, indicating that the vaccine induced heterologous T cell responses. Importantly, no clinical signs and undetectable virus shedding were observed after virulent PPRV challenge in vaccinated sheep. These vaccines also overcame the T cell immunosuppression induced by PPRV in control animals. The results indicate that these adenovirus constructs could be a promising alternative to current vaccine strategies for the development of PPRV DIVA vaccines.

    1. O princípio de razão, o utilitarismo e o antiutilitarismo

      Directory of Open Access Journals (Sweden)

      Alain Caillé

      2001-12-01

      Full Text Available Neste artigo, procura-se demonstrar que as noções de razão utilizadas nas ciências sociais são largamente determinadas pela tradição utilitarista e que, por conseguinte, na prática, o conceito de racionalidade tem limites discutíveis dados pela subordinação do mesmo à questão do cálculo interessado. Mas a crítica à racionalidade utilitarista é complexa na medida em que existem diferentes registros do utilitarismo: o prático, como é o caso do utilitarismo economicista que prega a satisfação primeira de interesses egoístas materiais; o teórico, que propõe serem todos os homens egoístas e calculistas por natureza; e o normativo, que vincula o ideal de justiça à satisfação do maior número de indivíduos. A crítica às teses utilitaristas leva o autor a lembrar a contribuição de Marcel Mauss na fundação de um novo paradigma, o da dádiva. Enfim, este texto é fundamental para se compreender a passagem de uma crítica antiutilitarista negativa para uma outra, antiutilitarista positiva, que se apoia na criação do novo paradigma.

    2. A invenção do “social” e a normatividade das Ciências Sociais: Dilemas clássicos e tendências contemporâneas

      Directory of Open Access Journals (Sweden)

      Anete B.L.Ivo

      2012-06-01

      Full Text Available O artigo discute as relações entre a sociologia e a questão social, explorando a dimensão cultural da ciência na organização da sociedade, na modernidade, na qual a sociologia é produto e produtora desta ordem. O artigo se estrutura em três partes: a primeira analisa a invenção da propriedade social do Estado na proteção social. A segunda contrapõe o homo sociologicus ao homo œconomicus, analisando a pauperização, como o fenômeno sobre o qual se constrói a crítica ao utilitarismo. A terceira apresenta três matrizes paradigmáticas sobre a pobreza na sociedade da abundância (Tocqueville, Marx & Engels, e Simmel. Na conclusão apresenta três paradigmas (o do contrato, do direito e da dádiva, que tentam refundar a proteção social de uma perspectiva do direito incondicional de todos os cidadãos a uma renda mínima universal.

    3. A Recombinant Rift Valley Fever Virus Glycoprotein Subunit Vaccine Confers Full Protection against Rift Valley Fever Challenge in Sheep.

      Science.gov (United States)

      Faburay, Bonto; Wilson, William C; Gaudreault, Natasha N; Davis, A Sally; Shivanna, Vinay; Bawa, Bhupinder; Sunwoo, Sun Young; Ma, Wenjun; Drolet, Barbara S; Morozov, Igor; McVey, D Scott; Richt, Juergen A

      2016-06-14

      Rift Valley fever virus (RVFV) is a mosquito-borne zoonotic pathogen causing disease outbreaks in Africa and the Arabian Peninsula. The virus has great potential for transboundary spread due to the presence of competent vectors in non-endemic areas. There is currently no fully licensed vaccine suitable for use in livestock or humans outside endemic areas. Here we report the evaluation of the efficacy of a recombinant subunit vaccine based on the RVFV Gn and Gc glycoproteins. In a previous study, the vaccine elicited strong virus neutralizing antibody responses in sheep and was DIVA (differentiating naturally infected from vaccinated animals) compatible. In the current efficacy study, a group of sheep (n = 5) was vaccinated subcutaneously with the glycoprotein-based subunit vaccine candidate and then subjected to heterologous challenge with the virulent Kenya-128B-15 RVFV strain. The vaccine elicited high virus neutralizing antibody titers and conferred complete protection in all vaccinated sheep, as evidenced by prevention of viremia, fever and absence of RVFV-associated histopathological lesions. We conclude that the subunit vaccine platform represents a promising strategy for the prevention and control of RVFV infections in susceptible hosts.

    4. Self-assembly and release of peste des petits ruminants virus-like particles in an insect cell-baculovirus system and their immunogenicity in mice and goats.

      Science.gov (United States)

      Li, Wenchao; Jin, Hongyan; Sui, Xiukun; Zhao, Zhanzhong; Yang, Chenghuai; Wang, Wenquan; Li, Junping; Li, Gang

      2014-01-01

      Peste des petits ruminants (PPR) is an acute, febrile, viral disease of small ruminants that has a significant economic impact. For many viral diseases, vaccination with virus-like particles (VLPs) has shown considerable promise as a prophylactic approach; however, the processes of assembly and release of peste des petits ruminants virus (PPRV) VLPs are not well characterized, and their immunogenicity in the host is unknown. In this study, VLPs of PPRV were generated in a baculovirus system through simultaneous expression of PPRV matrix (M) protein and hemaglutin in (H) or fusion (F) protein. The released VLPs showed morphology similar to that of the native virus particles. Subcutaneous injection of these VLPs (PPRV-H, PPRV-F) into mice and goats elicited PPRV-specific IgG production, increased the levels of virus neutralizing antibodies, and promoted lymphocyte proliferation. Without adjuvants, the immune response induced by the PPRV-H VLPs was comparable to that obtained using equivalent amounts of PPRV vaccine. Thus, our results demonstrated that VLPs containing PPRV M protein and H or F protein are potential "differentiating infected from vaccinated animals" (DIVA) vaccine candidates for the surveillance and eradication of PPR.

    5. Peste des petits ruminants.

      Science.gov (United States)

      Parida, S; Muniraju, M; Mahapatra, M; Muthuchelvan, D; Buczkowski, H; Banyard, A C

      2015-12-14

      Peste des petits ruminants virus causes a highly infectious disease of small ruminants that is endemic across Africa, the Middle East and large regions of Asia. The virus is considered to be a major obstacle to the development of sustainable agriculture across the developing world and has recently been targeted by the World Organisation for Animal Health (OIE) and the Food and Agriculture Organisation (FAO) for eradication with the aim of global elimination of the disease by 2030. Fundamentally, the vaccines required to successfully achieve this goal are currently available, but the availability of novel vaccine preparations to also fulfill the requisite for differentiation between infected and vaccinated animals (DIVA) may reduce the time taken and the financial costs of serological surveillance in the later stages of any eradication campaign. Here, we overview what is currently known about the virus, with reference to its origin, updated global circulation, molecular evolution, diagnostic tools and vaccines currently available to combat the disease. Further, we comment on recent developments in our knowledge of various recombinant vaccines and on the potential for the development of novel multivalent vaccines for small ruminants. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

    6. Non-redundant Functions of ATM and DNA-PKcs in Response to DNA Double-Strand Breaks

      Directory of Open Access Journals (Sweden)

      Pierre Caron

      2015-11-01

      Full Text Available DNA double-strand breaks (DSBs elicit the so-called DNA damage response (DDR, largely relying on ataxia telangiectasia mutated (ATM and DNA-dependent protein kinase (DNA-PKcs, two members of the PI3K-like kinase family, whose respective functions during the sequential steps of the DDR remains controversial. Using the DIvA system (DSB inducible via AsiSI combined with high-resolution mapping and advanced microscopy, we uncovered that both ATM and DNA-PKcs spread in cis on a confined region surrounding DSBs, independently of the pathway used for repair. However, once recruited, these kinases exhibit non-overlapping functions on end joining and γH2AX domain establishment. More specifically, we found that ATM is required to ensure the association of multiple DSBs within “repair foci.” Our results suggest that ATM acts not only on chromatin marks but also on higher-order chromatin organization to ensure repair accuracy and survival.

    7. Multi-locus fossil-calibrated phylogeny, biogeography and a subgeneric revision of the Margaritiferidae (Mollusca: Bivalvia: Unionoida).

      Science.gov (United States)

      Bolotov, Ivan N; Vikhrev, Ilya V; Bespalaya, Yulia V; Gofarov, Mikhail Y; Kondakov, Alexander V; Konopleva, Ekaterina S; Bolotov, Nikita N; Lyubas, Artyom A

      2016-10-01

      The taxonomy and biogeographic history of the bivalve family Margaritiferidae are controversial because previous molecular studies did not provide a well-resolved phylogenetic framework for these enigmatic freshwater mussels that have extensive but disjunct distribution in North America, Eurasia and North Africa. In this study, we present a new, fossil-calibrated phylogenetic hypothesis based on five molecular markers (∼4kb of total length) for ten species. Our results indicate that all recent margaritiferids are in the single genus, Margaritifera Schumacher, 1816. Additionally, we identified three relatively well-supported phylogenetic clades that are valid subgenera, i.e., Margaritifera s. str. (Holarctic), Margaritanopsis (=Cumberlandia) (southeast North America-southeast Asia disjunct) and Pseudunio (Mediterranean). We suggest that the crown lineage of the Margaritiferidae most likely originated in the Cretaceous (mean age 93Ma, 95% CI 66-126Ma). The combined results of ancestral area reconstructions based on the three different approaches (S-DIVA, DEC and S-DEC) showed that ancient vicariance events could have played an important role in speciation within the family. The rates of mitochondrial evolution of margaritiferids are notably slow, which may be associated with their longevity, long generation time and low metabolic rates. Our findings highlight the complex biogeographic history of the Margaritiferidae as an intermixing of ancient vicariance and dispersal events, which were most likely associated with some inland barriers, continental movements and a sea level dynamic.

    8. Atšķirības sieviešu ar augstu un zemu ķermeņa pašvērtējumu spriedumos par sabiedrībā populāru sieviešu ķermeņa izmēriem.

      OpenAIRE

      Kalniņa, Aija

      2009-01-01

      Pētījuma mērķis bija noskaidrot, vai pastāv atšķirības ar savu ķermeni neapmierinātu sieviešu un ar savu ķermeni apmierinātu sieviešu spriedumos par sabiedrībā populāru un medijos bieži redzamu sieviešu ķermeņu izmēriem. Pētījumā piedalījās divas izlases grupas: vienā bija 24 sievietes ar augstu ķermeņa pašvērtējumu, otrā – 25 sievietes ar zemu ķermeņa pašvērtējumu. Sievietes bija vecumā no 18 līdz 25 gadiem, kuru ķermeņa masas indekss (ĶMI) iekļaujas normālos rādītājos, un kurām nav ēšana...

    9. Gastronomia sensual: análise simbólica de A festa de Babette e Dona Flor e seus dois maridos = Sensual gastronomy: symbolic analysis of Babetes fiest and Dona Flor e seus dois maridos

      Directory of Open Access Journals (Sweden)

      Rocha, Gilmar

      2009-01-01

      Full Text Available O texto apresenta uma análise simbólica sobre a representação do gênero feminino dramatizada nos filmes Dona Flor e seus dois maridos (1976 e A festa de Babette (1987. Ambientados em contextos socio-históricos diferentes, tais filmes podem ser vistos como “etnografias” que falam do imaginário religioso e do ethos cultural protestante na Dinamarca de fins do século 19 e do sincretismo afro-brasileiro da primeira metade do século 20. O ponto de vista da antropologia simbólica, alimentada pelas contribuições teóricas de Clifford Geertz, Marcel Mauss, Victor Turner e Roberto DaMatta, orienta a interpretação dos filmes a partir dos códigos culturais da culinária (comida, da corporalidade (sexualidade e da religiosidade (puritanismo e sincretismo. Destaca-se, nessas “etnografias fílmicas”, o modo como os sistemas da dádiva e da carnavalização que as estruturam convergem para um mesmo campo de significação. A estratégia metodológica adotada consiste em comparar os filmes tendo por base a estrutura conceitual dos “dramas sociais”

    10. Development and evaluation of a replicon particle vaccine expressing the E2 glycoprotein of bovine viral diarrhea virus (BVDV in cattle

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      Loy John Dustin

      2013-01-01

      Full Text Available Abstract Background Bovine viral diarrhea virus is one of the most significant and costly viral pathogens of cattle worldwide. Alphavirus-derived replicon particles have been shown to be safe and highly effective vaccine vectors against a variety of human and veterinary pathogens. Replicon particles are non-propagating, DIVA compatible, and can induce both humoral and cell mediated immune responses. This is the first experiment to demonstrate that Alphavirus-based replicon particles can be utilized in a standard prime/boost vaccination strategy in calves against a commercially significant bovine pathogen. Findings Replicon particles that express bovine viral diarrhea virus sub-genotype 1b E2 glycoprotein were generated and expression was confirmed in vitro using polyclonal and monoclonal antibodies specific to E2. Vaccine made from particles was generated in Vero cells and administered to BVDV free calves in a prime/boost regimen at two dosage levels. Vaccination resulted in neutralizing antibody titers that cross-neutralized both type 1 and type 2 BVD genotypes following booster vaccination. Additionally, high dose vaccine administration demonstrated some protection from clinical disease and significantly reduced the degree of leukopenia caused by viral infection. Conclusions Replicon particle vaccines administered in a prime/boost regimen expressing BVDV E2 glycoprotein can induce cross-neutralizing titers, reduce leukopenia post challenge, and mitigate clinical disease in calves. This strategy holds promise for a safe and effective vaccine to BVDV.

    11. Evolución biogeográfica de los Pachydeminae paleárticos (Coleoptera, Scarabaeoidea mediante análisis de dispersión-vicarianza

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      Sanmartín, Isabel

      2003-12-01

      Full Text Available The beetle subfamily Pachydeminae Reitter, 1902 is one of the least-known subfamilies of Melolonthidae or “leaf-chafers” (Coleoptera, Scarabaeoidea. Some species of Pachydeminae have recently been described as agricultural pests of olive trees. The Pachydeminae are distributed in all major zoogeographical regions (except Australia and India but their distribution is very disjunct. In the Palearctic region, they are distributed across southern Eurasia from the Canary Islands to China, including southern Europe (except France and Italy, North Africa, Asia Minor, Middle East, Iran-Afghanistan, Caucasus, and Central Asia. The majority of species occur in the southwestern Palearctic, with only a few species in China. As in the rest of Melolonthidae subfamilies (Browne & Scholtz, 1999, phylogenetic relationships within the Pachydeminae are poorly resolved. Recently, Sanmartín & Martín-Piera, (2003 reviewed the systematics of the Palearctic genera, and proposed the first phylogenetic hypothesis within the subfamily. This study summarizes the conclusions of Sanmartín (1998 and Sanmartín (2003, which reconstructed the biogeographic history of the subfamily Pachydeminae in the Palearctic region using dispersalvicariance analysis (DIVA, Ronquist, 1996, 1997. This method reconstructs the ancestral distribution in a given phylogeny based on a vicariance model, while allowing dispersal and extinction to occur. Unlike other methods, DIVA does not enforce area relationships to conform to a hierarchical “area cladogram” so it can be used to reconstruct “reticulate” biogeographic scenarios. DIVA optimal reconstructions suggest that the ancestor of Pachydeminae was originally present in the south-eastern Mediterranean region, including North Africa, the Middle East, the Iranian Plateau, and the Balkans/Anatolian region. During the Oligocene-Miocene, the collision between the Arabian, African, and Eurasian Plates resulted in the appearance of

    12. Oportuna advertencia a los Médicos.

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      Asociación Medica Colombiana AMC

      1999-04-01

      Full Text Available

      La Junta Directiva de la Asociación Médica Colombiana consideró conveniente enviar a todos los colegas del país la Circular que reproducimos y con cuyos términos está plenamente de acuerdo la Academia Nacional de Medicina. La defensa de la calidad de nuestro ejercicio. y de la correcta imagen profesional. son indispensables y requieren la permanente participación y vigilancia de todo el Cuerpo Médico Colombiano.


      De: Junta Directiva Asociación Médica Colombiana AMC
      Para: Todos los distinguidos Colegas


      Muy estimados colegas y amigos:


      De acuerdo con la voluntad de ustedes, que de modo individual conforman la Asociación Médica Colombiana y que nos han dado la honrosa tarea de liderar el trabajo en favor de nuestra profesión en todos los terrenos, esta Junta Directiva considera deber urgente el de recordar, en la más explícita forma, que contraría los dictados de la Etica y atenta gravemente contra la calidad del ejercicio el aceptar cualquier clase de incentivo o dádiva -salvo los justos honorarios, previamente convenidos, por el Acto Médico- a cambio de la prescripción de medicamentos, la indicación de exámenes o cualquiera otra parte del Acto Médico que deba ser realizada por personas distintas del propio profesional o con el concurso de ellas. Es bien conocida la prohibición de "dicotomía", es decir, de reparto de honorarios entre el profesional que remite su paciente a un colega y este colega; pero resulta igualmente
      inaceptable el percibir dinero u objetos de valor, agasajos o dádivas, por el hecho de recetar determinado medicamento o hacer alguna indicación dentro del estudio o tratamiento de un paciente, ya que tales acciones deben tomar en cuenta únicamente la realidad clínica del enfermo y su adecuado

    13. Combinação da altura de desponte e do espaçamento entre plantas de tomate Combination of planting densities with top lopping heights of tomato plants

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      Siegfried Mueller

      2009-03-01

      Full Text Available Em trabalhos a campo realizados na Epagri, Estação Experimental de Caçador (SC, durante as safras de 1998/1999, 1999/2000 e 2000/2001, estudou-se a interação entre quatro alturas de desponta (acima do 5º, 7º, 9º e 11º cachos e quatro espaçamentos entre plantas (0,30; 0,45; 0,60 e 0,75 m, de plantas de tomate cv. Diva. O espaçamento entre fileiras foi de 1,0 m para todos os tratamentos e anos estudados. O delineamento experimental foi de blocos completos ao acaso, em um fatorial 4 x 4, com três repetições. As plantas foram tutoradas em 'V' invertido empregando-se varas de bambu (taquaruçú como tutores. Foram avaliados a produtividade e o número de frutos total e comercial, a percentagem e a massa média de frutos comercial, a altura de plantas e o diâmetro da base do caule. O aumento do espaçamento de plantas reduziu a altura das plantas, o número e a produtividade de frutos comercial e total, porém proporcionou aumento do diâmetro do caule e da massa média dos frutos comerciais. Por sua vez, o aumento do número de cachos por planta aumentou a altura das plantas, o número e a produtividade de frutos comercial e total. Entretanto, o diâmetro do caule e a massa média dos frutos comerciais diminuíram com o aumento do número de cachos por planta.Three field experiments were carried out in Caçador, Santa Catarina State, Brazil, during 1998/1999, 1999/2000 and 2000/2001 seasons to study four lopping heights of the tops (above the 5th, 7th, 9th, and 11th clusters and four-plant spacings between plants (0.30; 0.45; 0.60, and 0.75 m. Plant spacing between rows of tomato cv. Diva was of 1.0 m, in all studied treatments and years of the research. Experiments were designed in randomized blocks, with three replicates in a factorial scheme (4 x 4. Plants were staked as crossed fence, using bamboo sticks as tutors. The yield, number of fruits, percentage and average weight of marketable fruits, plant height and stem base diameter

    14. 嗓音障碍指数主观评估与发音障碍严重程度指数客观检测的相关性分析%Correlation between voice handicap index and voice acoustic analysis in patients with vocal cord polyps

      Institute of Scientific and Technical Information of China (English)

      李晓雨; 李进让

      2016-01-01

      目的:探讨声带息肉患者嗓音障碍指数(voice handicap index,VHI)量表自我评估和发音障碍严重程度指数(dysphonia severity index,DSI)客观评估的相关性。方法取104例声带息肉患者,用VHI中文版量表进行自评及DIVAS2.5声学分析软件分析DSI,按性别、侧别、形状、大小分组比较,分析VHI和DSI的相关性。结果声带息肉的大小、形状对VHI各指标差异均无统计学意义,但较大或广基底形息肉基频微扰较重;女性患者功能、生理和VHI总体评价高于男性,但DSI却低于男性;双侧息肉比单侧在生理方面影响较重,同时DSI也较重;VHI量表各指标与嗓音声学分析各参数之间无明显相关性。结论目前临床上评估嗓音障碍程度的两种主客观方法都不理想,应进一步研究更好的评价方法。%[ABSTRACT]OBJECTIVETo investigate the relationship between self-assessment of Voice Handicap Index (VHI) and objective detection of Dysphonia Severity Index (DSI) in vocal ford polyp patients.METHODSAll 104 vocal cord polyp patients assessed the Chinese version of VHI scale by themselves. The DSI was calculated by acoustic analysis of DIVAS2.5. The correlation between VHI and DSI was analyzed in Pearson test.RESULTS There was no significant difference in each index of VHI scale score by side and shape, but the Shimmer of larger or wide-base polyps was more severe. The female patients' function, physiology, and VHI total score were higher than that of male patients, but the DSI was lower than that of male patients. The physiology score was higher in bilateral vocal cord polyp patients, so as well as DSI. There was no significant correlation between each item of VHI scale score and DSI.CONCLUSIONNeither the current subjective VHI nor objective DSI in clinical assessment of voice disorders disability is ideal, further research and better evaluation methods should be further explored.

    15. Observation of the dysphonia severity index in evaluating curative effect of vocal cord polyp surgery%嗓音障碍严重指数评价声带息肉手术疗效的研究

      Institute of Scientific and Technical Information of China (English)

      周舟; 葛平江; 刘倩; 刘明; 张伟

      2015-01-01

      目的 应用嗓音障碍严重指数(dysphonia severity index,DSI)对声带息肉患者手术治疗效果进行评估,讨论DSI评估嗓音障碍程度的有效性和可靠性.方法 本研究选取正常人(34名)与声带息肉患者(70例),分别进行嗓音障碍指数(voice handicap index,VHI)评估、GRBAS评分、纤维电子喉镜检查,测量最大声时(maximum phonatory time,MPT)、jitter、shimmer及DSI值.运用方差分析DSI值在两组及手术前后组间的差异,并用VHI、GRBAS评分、shimmer与DSI值做相关性分析.结果 70例患者术前DSI值均值为(-2.92±1.59),术后均值(1.87±1.33),正常组均值为(2.30±2.16),术前与术后的DSI值,以及正常组与患者术前DSI均值比较差异均有统计学意义(P<0.05).通过Pearson相关分析,得到DSI值与VHI值(r=-0.632),G值(r=-0.740),R值(r=-0.756),shimmer(r=-0.500)之间有相关关系(P<0.01).结论 DSI可有效地评估声带息肉患者的病理嗓音障碍程度,可作为一个独立使用的评估嗓音障碍程度的多参数方法,并可对声带息肉的手术疗效进行客观评价.%Objective To investigate the applicability of the eysphonia severity index (DSI) in evaluating effects of surgery between before and after groups of vocal polyp patients.Methods Analyses of measurement data pre and pro-surgery of 70 vocal polyp patients and 35 no voice disorders volunteers(control group).The voice quality was measured subjectively with the voice handicap index(VHI),the GRBAS and fiber electronic laryngoscopy.Measures of maximum phonation time (MPT),shimmer and jitter were obtained for each subject by using DiVAS 2.30 (XION,Germany).The DiVAS 2.30 had spotanenously calculate the scores of DSI.Using SPSS 17.0 to find the differences of DSI scores among the three groups by one-way ANOVA variance analysis.And finding out of the correlation with DSI scores and VHI scores,GRBAS,MPT,jitter and shimmer.Results DSI improved significantly after surgery in the vocal polyps

    16. 声带息肉患者手术前后VHI量表主观评估和 DSI 嗓音检测结果分析%The Significance of the Self -assessments of Voice Handicap Index and the Detection of Dysphonia Severity Index in Polyp of Vocal Cord

      Institute of Scientific and Technical Information of China (English)

      张武宁; 唐安洲; 徐志文; 吴铖林; 李永湘; 钟晖; 毛海燕

      2013-01-01

      Objective To explore the significance of the Self -assessments of Voice Handicap Index (VHI) and The objective detection of Dysphonia Severity Index (DSI) in polyp of vocal cord perioperatively .Methods The Self -assessments of VHI had been completed in 28 patients with polyp of vocal cord before the operation and one week after the operation ,including function(F) ,physiological(P) ,emotion(E) ,and the sum denoted by T .In the meantime ,The DSI had been completed in these patients by acoustic analysis of DIVAS 2 .5 .After one month ,19 pa-tients had been re -examined .Results The DSI value was increasing ,but there was no significant difference be-tween pre-operation and one week after the operation(P>0 .05) ,indicating that the function of vocal cord was not recovered .In VHI scale ,the parameters were significantly different between the time preoperatively and one week postoperatively in F ,E and T(P0 .05) ,indicating that the function of vocal cord was not completely recovered as same as the result of DSI .After one month ,there were significant differences in DSI and VHI ,which showed a full recovery of vocal cord fanction .Conclusion The Self -assessments of VHI and The objective detection of DSI in polyp of vocal cord can evaluate simultaneouly the function of laryngeal .Additionally ,there was a good correlation between the DSI and the VHI score .%目的探讨声带息肉患者手术前后嗓音障碍指数(voice handicap index ,VHI)量表自我评估和嗓音障碍严重指数(dysphonia severity index ,DSI)评估的价值。方法对28例声带息肉患者手术前及手术后1周、术后1月(19例)进行VHI量表自我评估,并运用DIVAS2.5声学分析软件评估DSI ,分析其结果。结果与术前比较,声带息肉患者术后1周DSI值升高,但差异无统计学意义(P>0.05);VHI量表中,功能F、情感E、总分T得分较术前降低,差异有统计学意义(P<0.05),而生理P得分

    17. 白花油麻藤的地理分布及适生区预测%Research on geographic distribution and potential distribution of Mucuna birdwoodiana

      Institute of Scientific and Technical Information of China (English)

      张蓝月; 叶向斌; 刘念; 赵华

      2012-01-01

      白花油麻藤是豆科黧豆属大型木质藤本植物.运用Diva-Gis软件,结合海拔高度图层和植被图层绘制了白花油麻藤的地理分布图,分析了白花油麻藤的分布规律和生境特性;以Maxent模型作为物种适生性预测模型,温度和降水作为预测的环境因子,预测了白花油麻藤在中国的适生区.结果表明:白花油麻藤在中国27.5°N以南中低海拔10~1 200 m有分布,如山坡、路旁、沟谷、溪边及林下灌丛;喜温暖湿润气候,广东为白花油麻藤分布最为密集的地区.白花油麻藤的分布与植被类型和海拔有着密切的关系,分布区的植被类型为亚热带常绿阔叶林和热带季雨林,随着植被分布密度的降低和海拔的升高白花油麻藤的分布范围和分布密度呈逐渐缩小的趋势.白花油麻藤在中国的潜在分布区为粤、桂、闽、港、澳、滇、琼、赣、川、黔、藏、湘、浙等省区及交界处,其种质资源的保存及其利用应考虑其潜在分布区.%Mucuna birdvuoodiana in the genus mucuna,is large woody vine. In this paper,the geographic distribution maps of M. birdvuoodiana were drawn,and its distribution regularities and habitat characteristics were also analyzed by virtue of Diva-Gis software on altitude layer and vegetation layer. The natural potential distribution regions of M. birdvuoodiana in China were estimated based on the Maxent model as prediction model, temperature and precipitation as environment variables. The results showed that Mucuna birdwoodiana was distributed from low to medium altitude of south China below 27. 5°N,such as mountain-slope,wayside,cleugh,stream and underbrush. M. birdwoodi-ana,preferring to warmth humid climate, was enriched in Guangdong Province. The geographical distribution of M. birdwoodiana was closely related to vegetation type and altitude. The vegetation types of distribution region were mainly subtropical evergreen broadleaved forest and tropical

    18. Caracterização parcial de um Tymovirus isolado de tomateiros Partial characterization of a Tymovirus isolated from tomato

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      Isabel Cristina Batista

      2012-01-01

      Full Text Available Um Tymovirus isolado de sementes de tomateiro (Solanum lycopersicum 'Paronset' com sintomas de necrose, denominado TyToRS08, foi caracterizado quanto ao círculo de hospedeiras, propriedades físico-químicas, sorológicas e moleculares. O vírus foi transmitido mecanicamente a partir de folhas, frutos e sementes infectadas. A gama de hospedeiras incluiu espécies de Amaranthaceae, Chenopodiaceae e Solanaceae, induzindo sintomas local e sistêmicos na maior parte das espécies, exceto para Nicotiana sylvestris, N. tabacum 'Samsun', 'White Burley' e 'Xanthi' que não manifestaram sintomas. Em tomateiros 'Alambra', 'Thomas', 'Diva', 'Romana' e 'Graziela', o vírus induziu sintomas de mosaico e necrose branca. A presença do vírus foi detectada, nas sementes infectadas de todas as cultivares avaliadas, porém não se notou em plântulas após a germinação destas sementes. Foi obtida uma preparação purificada com concentração de 1,04 mg mL-1, empregada na produção de um antissoro policlonal que reagiu em PTA-ELISA com um título de 1/32768. Utilizou-se um par de oligonucleotídeos degenerados, desenhados para anelar na ORF 1 de espécies de Tymovirus, que permitiu a amplificação por RT-PCR de fragmentos com cerca de 700 pb. Após o alinhamento e a análise das sequências, verificou-se que o isolado TyToRS08 possuía valores abaixo de 70% de identidade com as espécies de Tymovirus, indicando que pode se tratar de uma espécie distinta das descritas no gênero, com potencial epidemiológico, pela sua estabilidade e disseminação por sementes de tomate.A Tymovirus isolated from seeds of tomato (Solanum lycopersicum cv. 'Paronset' with necrosis, named TyToRS08 isolate, was characterized by its host range, physical-chemical, serological and molecular properties. Firstly, it was successfully transmitted by mechanical inoculation by infected seeds, and the host range was restricted to species of Amaranthaceae, Chenopo diaceae and Solanaceae

    19. Distribution and agroclimatic characterization of potential cultivation regions of physic nut in Mexico Distribuição e caracterização agroclimática de regiões potenciais para cultivo de pinhão-manso no México

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      Carlos Alberto Núñez-Colín

      2009-09-01

      Full Text Available The purposes of this study were to determine the distribution and climatic patterns of current and future physic nut (Jatropha curcas cultivation regions in Mexico, and to identify possible locations for in vivo germplasm banks establishment, using geographic information systems. Current climatic data were processed by Floramap software to obtain distribution maps and climatic patterns of regions where wild physic nuts could be found. DIVA-GIS software analyzed current climatic data (Worldclim model and climatic data generated by CCM3 model to identify current and future physic nut cultivation regions, respectively. The distribution map showed that physic nut was present in most of the tropical and subtropical areas of Mexico, which corresponded to three agroclimatic regions. Climate types were Aw2, Aw1, and Bs1, for regions 1, 2 and 3, respectively. Nontoxic genotypes were associated with region 2, and toxic genotypes were associated with regions 1 and 3. According to the current and future cultivation regions identified, the best suitable ones to establish in vivo germplasm collections were the coast of Michoacán and the Isthmus of Tehuantepec, located among the states of Veracruz, Oaxaca and Chiapas.Os objetivos deste estudo foram determinar a distribuição e os padrões climáticos de regiões de cultivo, atuais e futuras, do pinhão-manso no México, e identificar possíveis locais para o estabelecimento de bancos ativos de germoplasma, com uso de sistemas de informação geográfica. Os dados climáticos atuais foram processados pelo software Floramap, para obter mapas de distribuição e padrões climáticos de regiões onde plantas de pinhão-manso selvagens poderiam ser encontradas. Foi utilizado o software DIVA-GIS para análise dos dados climáticos atuais (modelo Worldclim e dos dados climáticos gerados pelo modelo CCM3, para identificar regiões de cultivo atuais e futuras de pinhão-manso, respectivamente. O pinhão-manso est

    20. Epitope Mapping of Avian Influenza M2e Protein: Different Species Recognise Various Epitopes.

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      Noor Haliza Hasan

      Full Text Available A common approach for developing diagnostic tests for influenza virus detection is the use of mouse or rabbit monoclonal and/or polyclonal antibodies against a target antigen of the virus. However, comparative mapping of the target antigen using antibodies from different animal sources has not been evaluated before. This is important because identification of antigenic determinants of the target antigen in different species plays a central role to ensure the efficiency of a diagnostic test, such as competitive ELISA or immunohistochemistry-based tests. Interest in the matrix 2 ectodomain (M2e protein of avian influenza virus (AIV as a candidate for a universal vaccine and also as a marker for detection of virus infection in vaccinated animals (DIVA is the rationale for the selection of this protein for comparative mapping evaluation. This study aimed to map the epitopes of the M2e protein of avian influenza virus H5N1 using chicken, mouse and rabbit monoclonal or monospecific antibodies. Our findings revealed that rabbit antibodies (rAbs recognized epitope 6EVETPTRN13 of the M2e, located at the N-terminal of the protein, while mouse (mAb and chicken antibodies (cAbs recognized epitope 10PTRNEWECK18, located at the centre region of the protein. The findings highlighted the difference between the M2e antigenic determinants recognized by different species that emphasized the importance of comparative mapping of antibody reactivity from different animals to the same antigen, especially in the case of multi-host infectious agents such as influenza. The findings are of importance for antigenic mapping, as well as diagnostic test and vaccine development.

    1. ‘Suheir Hammad’s Negotiated Historiography of Arab America

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      Sally Michael Hanna

      2015-05-01

      Full Text Available The paper deals with the rise and restoration of the Arab American agency from a place of potential exile into a negotiated space of cultural existence. The attempt to create a realm of art that negotiates ethnic and racial specifity as it carves a space for itself represents the foundational logic of Suheir Hammad’s artistry. The study argues that objectification in Arab American representation conditions the psychotic foreclosure of history of the Arab American self. It is a process premised upon the inability to assimilate historical precepts- not that history does not exist, but that one’s present perception falls short of envisioning it into wholesomeness, owing to a breach, or a willful rejection of an incompatible instance that occurred in the past.   A confrontation with western historiography as the normative mode of narrativization is suggested in the study as a necessary starting point for reclaiming an authentic version of a true Arab American past. The reclamation of a mediated history of self and otherness outside of the patriarchal structure of western hegemony and the initiation of a relationship with the language of the Diaspora through hybridization lead to the conditioned birth of a new Arab American voice consciousness. A deconstructive negotiated historiography should deal with a direct engagement with difference not as a contested category of discord but as one that allows for a trajectory of cultural and historical emergence and intellectualization of vistas of Arab culture. The paper postulates that Suheir Hammad’s Born Palestinian Born Black, Breaking Poems and Zataar Diva rearticulate such negotiated historiography of Arab American cultural consciousness. Analysis of selected poems from the three volumes locate such new cultural consciousness. Key words: Arab American Agency- narrativization- historiography- wholesomeness- hybridization

    2. Dimensões do associativismo voluntário no cenário das relações entre saúde, pobreza e doença The dimensions of volunteer associativism in the scenario of the relationships amongst health, poverty and disease

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      Martha Cristina Nunes Moreira

      2010-05-01

      Full Text Available O presente artigo trabalha com a temática do associativismo voluntário tomando por base o aporte teórico da teoria da dádiva (1960. Em sua base empírica, estão os dados primários advindos de uma pesquisa realizada com dirigentes e/ou fundadores de catorze associações voluntárias brasileiras identificadas por uma filosofia de ação comum. A discussão analisa a ação dessas associações e outras ações voluntárias também surgidas no cenário brasileiro da década de noventa do século XX. Destacamos como conclusão o fato de que às iniciativas voluntárias é preciso ancorar a perspectiva de um circuito de trocas, de proximidade e reciprocidade, às custas de recairmos em ações de benemerência, que reativam a filantropia e a dependência.The present article discusses the theme of volunteer associativism based on the theoretical foundation of the theory of donation (1960. In his empirical base are the primary data of a research carried out with leaders and/or founders of 14 Brazilian voluntary associations identified by a common action philosophy. The discussion analyzes the accomplishments of those associations and other voluntary actions also arisen in the 90s in Brazil. The necessity of anchoring to volunteer initiatives the perspective of circuit of exchange, proximity and reciprocity is highlighted in order to avoid relapses to merit actions which reactivate philanthropy and dependence is highlighted as a central conclusion.

    3. Global Foot-and-Mouth Disease Research Update and Gap Analysis: 4 - Diagnostics.

      Science.gov (United States)

      Knight-Jones, T J D; Robinson, L; Charleston, B; Rodriguez, L L; Gay, C G; Sumption, K J; Vosloo, W

      2016-06-01

      This study assessed knowledge gaps in foot-and-mouth disease (FMD) research in the field of diagnostics. The study took the form of a literature review (2011-15) combined with research updates collected in 2014 from 33 institutes from around the world. Findings were used to identify priority areas for future FMD research. Molecular and genetic technologies, including sequencing, are developing at an increasing rate both in terms of capability and affordability. These advances potentiate progress in many other fields of research, from vaccine development to epidemiology. The development of RT-LAMP represents an important breakthrough allowing greater use and access to molecular diagnostics. It is now possible to determine virus serotype using PCR, although only for certain virus pools, continued progress is needed to cover the global spectrum of FMD viruses. Progress has also been made in the development of pen-side rapid diagnostics, some with the ability to determine serotype. However, further advances in pen-side serotype or strain determination would benefit both FMD-free countries and endemic countries with limited access to well-resourced laboratories. Novel sampling methods that show promise include air sampling and baited ropes, the latter may aid sampling in wildlife and swine. Studies of infrared thermography for the early detection of FMD have not been encouraging, although investigations are ongoing. Multiplex tests have been developed that are able to simultaneously screen for multiple pathogens with similar clinical signs. Crucial for assessing FMDV freedom, tests exist to detect animals that have been infected with FMDV regardless of vaccination status; however, limitations exist, particularly when testing previously vaccinated animals. Novel vaccines are being developed with complementary DIVA tests for this purpose. Research is also needed to improve the current imprecise approaches to FMD vaccine matching. The development of simple, affordable

    4. La categoría de don en el marco de la economía social y solidaria

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      Susana Presta

      2007-12-01

      Full Text Available La crisis de los años '90 ha revitalizado los preceptos de la Economía Social propiciando distintos emprendimientos cooperativos. Este artículo analiza el caso de una Cooperativa Educacional y su Banco de Horas desde la perspectiva de la categoría de don , para ahondar no sólo en el análisis de la construcción de relaciones sociales al interior de dicha organización socioeconómica, sino también avanzar sobre las formas en que la misma se vincula con distintas corporaciones transnacionales a partir de diversas donaciones.A crise do '90 criou os preceitos da Economia Social como uma guia a projetos cooperativos diferentes. Neste artigo, analisamos uma Cooperativa Educativa e seu Banco de Horas dentro da perspectiva da categoria da dádiva, para aumentar a nossa compreensão sobre a construção de relações sociais dentro desta organização socioeconômica, mas alem tentaremos um analise das relações entre o Banco de Horas e as corporações transnacionais diferentes que lhe fazem doações.The crisis of the 90 th has brought up the precepts of the Social Economy as a guide to different cooperative projects. In this article, we analyze an Educational Cooperative and its Hours Bank within the perspective of the category of gift in order to increase our comprehension about the social relations construction inside this socioeconomic organization, but also we will try to go further into the relations between the Hours Bank and the different transnational corporations that make donations to them.

    5. The Eruption Forecasting Information System (EFIS) database project

      Science.gov (United States)

      Ogburn, Sarah; Harpel, Chris; Pesicek, Jeremy; Wellik, Jay; Pallister, John; Wright, Heather

      2016-04-01

      The Eruption Forecasting Information System (EFIS) project is a new initiative of the U.S. Geological Survey-USAID Volcano Disaster Assistance Program (VDAP) with the goal of enhancing VDAP's ability to forecast the outcome of volcanic unrest. The EFIS project seeks to: (1) Move away from relying on the collective memory to probability estimation using databases (2) Create databases useful for pattern recognition and for answering common VDAP questions; e.g. how commonly does unrest lead to eruption? how commonly do phreatic eruptions portend magmatic eruptions and what is the range of antecedence times? (3) Create generic probabilistic event trees using global data for different volcano 'types' (4) Create background, volcano-specific, probabilistic event trees for frequently active or particularly hazardous volcanoes in advance of a crisis (5) Quantify and communicate uncertainty in probabilities A major component of the project is the global EFIS relational database, which contains multiple modules designed to aid in the construction of probabilistic event trees and to answer common questions that arise during volcanic crises. The primary module contains chronologies of volcanic unrest, including the timing of phreatic eruptions, column heights, eruptive products, etc. and will be initially populated using chronicles of eruptive activity from Alaskan volcanic eruptions in the GeoDIVA database (Cameron et al. 2013). This database module allows us to query across other global databases such as the WOVOdat database of monitoring data and the Smithsonian Institution's Global Volcanism Program (GVP) database of eruptive histories and volcano information. The EFIS database is in the early stages of development and population; thus, this contribution also serves as a request for feedback from the community.

    6. Decrease of the Black Sea Oxygen Inventory through the second half of the XXth century

      Science.gov (United States)

      Capet, Arthur; Stanev, Emil; Gregoire, Marilaure

      2015-04-01

      We investigated the dynamics of the oxygen inventory and penetration depth in the Black Sea over the period 1955-2013 using CTD and ARGO profiles. Several authors (e.g. Codispoti et al., 1991; Konovalov and Murray, 2001; Stanev et al., 2013) have proposed that the upper interface of the suboxic layer has shoaled on a density scale during the last decades in answer to eutrophication. Those studies are based on the assumption that oxygen vertical properties are sufficiently stable, spatially and seasonally, when expressed on a density scale to permit representative interannual statistics. We used the Data Interpolating Variational Analysis (DIVA) software and in particular the de-trending algorithm (Capet et al. 2014) to explicitly evaluate the spatial, seasonal and interannual variabilities of the total Oxygen vertical content and penetration depth (in depth and density scales). The most striking result is the strong and quasi-monotonous decrease of the Black Sea total oxygen inventory which, in 2013, only amounts to 70% of what it was in 1955. The study also confirms a shoaling of the [O2]=20 mmol/m3 iso-surface, on the order of 8m (0.06 kg/m3) per decade. Profiles obtained from two Argo float (2010-2012) confirmed the present status of the properties obtained from CTD cast, and were used to assess the role of the Cold Intermediate Layer in ventilating the upper part of the Black Sea open basin. It effectively appears that the important dense water formation in the period 1985-1995 refrained the upper-mentioned trends and that the lower formation in 1995-2010 has contributed to their more recent increases.

    7. A new global dataset with extreme sea levels and its application for assessing flood risk

      Science.gov (United States)

      Muis, Sanne; Verlaan, Martin; Winsemius, Hessel; Aerts, Jeroen; Ward, Philip

      2016-04-01

      Extreme sea levels, caused by storm surges and high tides, can have devastating societal impacts. The global coastal population is faced with an increasing trend in flood risk, induced by socio-economic development and climate change. Without action, the increasing trends in flood hazard and exposure will be associated with catastrophic flood losses in the future. The adequate allocation of global investments in adaptation requires an accurate understanding of the current and future coastal flood risk on a global-scale. Here we present the first global reanalysis of storm surges and extreme sea levels (GTSR dataset) based on dynamical modelling. GTSR covers the entire world's coastline and consists of time series of tides and surges and estimates of extreme values for various return periods. The dataset is based on two different hydrodynamic models: FES2012 for modelling tides, and GSTM for modelling storm surges. GSTM is forced by meteorological fields from ERA-Interim to simulate storm surges for the period 1979-2014. Validation showed that there is very good agreement between modelled and observed sea levels. Only in regions prone to tropical cyclones, extreme sea levels are severely underestimated due to the limited resolution of the meteorological forcing. This will be resolved for future updates of GTSR. As a first application of GSTR, we estimate that 99 million people are exposed to a 1 in 100 year flood. This is almost 40% lower than estimates based the DIVA dataset, another global dataset of extreme sea level. We foresee other applications in assessing impacts of climate change and risk management, such as assessing changes in storminess, estimating the impacts of sea level, and providing warning levels to operational models.

    8. Marine phages as excellent tracers for reactive colloidal transport in porous media

      Science.gov (United States)

      Ghanem, Nawras; Chatzinotas, Antonis; Harms, Hauke; Wick, Lukas Y.

      2016-04-01

      Question: Here we evaluate marine phages as specific markers of hydrological flow and reactive transport of colloidal particles in the Earth's critical zone (CZ). Marine phages and their bacterial hosts are naturally absent in the CZ, and can be detected with extremely high sensitivity. In the framework of the DFG Collaborative Research Center AquaDiva, we asked the following questions: (1) Are marine phages useful specific markers of hydrological flow and reactive transport in porous media? and (2) Which phage properties are relevant drivers for the transport of marine phages in porous media? Methods: Seven marine phages from different families (as well two commonly used terrestrial phages) were selected based on their morphology, size and physico-chemical surface properties (surface charge and hydrophobicity). Phage properties were assessed by electron microscopy, dynamic light scattering and water contact angle analysis (CA). Sand-filled laboratory percolation columns were used to study transport. The breakthrough curves of the phages were analyzed using the clean bed filtration theory and the XDLVO theory of colloid stability, respectively. Phages were quantified by a modified high- throughput plaque assay and a culture-independent particle counting method approach. Results: Our data show that most marine tested phages exhibited highly variable transport rates and deposition efficiency, yet generally high colloidal stability and viability. We find that size, morphology and hydrophobicity are key factors shaping the transport efficiency of phages. Differing deposition efficiencies of the phages were also supported by calculated XDLVO interaction energy profile. Conclusion: Marine phages have a high potential for the use as sensitive tracers in terrestrial habitats with their surface properties playing a crucial role for their transport. Marine phages however, exhibit differences in their deposition efficiency depending on their morphology, hydrophobicity and

    9. Characterisation of enzymatic activities of H5N1 influenza virus

      Directory of Open Access Journals (Sweden)

      Simson Tarigan

      2008-06-01

      Full Text Available One of the two glycoproteins projected from the surface of the influenza virus is identified as neuraminidase. This enzyme enables the virus to spread in the host, and therefore it plays vital roles in the viral pathogenicity. From the viewpoint of disease control, neuraminidase is used as the target for the development of anti-flu drugs, and for the development of diagnostic test to differentiate infected from vaccinated animals (DIVA. Since the roles of the enzyme are very important, information regarding the characteristics and the procedure to measure its activity, which is the purpose of this study, is essential. The optimum incubation time of the neuraminidase-substrate (fetuin reaction and the optimum pH of the buffer were determined. The stability of the enzyme against heating, supplementation or chelating of calcium ion, and b-propiolactone treatment were analysed. This study showed that neuraminidase from H5N1-influenza virus was, in regards to the characteristics investigated in this study, was comparable to that from Clostridium perfringens. The optimum incubation time for the viral and Clostridial neuraminidases were 60 and 30 minutes, respectively; whereas, the optimum pH for both neuraminidase was 6-7. At pH 8, both neuraminidase were inactive. Supplementation of calcium ion tended to increase activity but chelating of the cation did not have any observable effects. Treatment with 0.2% b-propiolactone for 6 hours reduced the activity, whereas heating at 60°C for 60 minutes abolished all activity. Since inactivation by b-propiolactone is partially only, neuraminidase assay could be performed safely in ordinary laboratories using b-propiolactone-treated-influenza virus, rather than the life virus. The thermolabile nature of the enzyme will complicate any attempt to purify the enzyme.

    10. Diversification of almonds, peaches, plums and cherries - molecular systematics and biogeographic history of Prunus (Rosaceae).

      Science.gov (United States)

      Chin, Siew-Wai; Shaw, Joey; Haberle, Rosemarie; Wen, Jun; Potter, Dan

      2014-07-01

      Most previous molecular phylogenetic studies of Prunus have been conducted primarily with crop species and their close relatives. As the center of crop diversity of the genus is in Eurasia, the geographic origin of Prunus has inevitably been inferred to be Eurasia as well. The lesser-known tropical Prunus species have not been well represented in previous phylogenetic reconstructions; therefore, their effects on inferences about the phylogenetic structure and geographic origin of Prunus are uncertain. In this study, we examined the phylogeny of Prunus, including an expanded sampling of species from tropical regions in Southeast Asia and the Americas, using sequences from four plastid markers and the nuclear ribosomal ITS region. A penalized likelihood method was used to estimate the absolute age of Prunus and the timing of infrageneric cladogenic events. The geographic origin of Prunus and ancestral sites of cladogenesis were inferred using the Bayes-DIVA approach. Our results indicate that the modern genus appeared ∼61Myr in eastern Asia and that diversification of all major lineages may have been triggered by the global warming period of the early Eocene. In addition, our molecular dating estimates suggest that the crown clade that includes the temperate deciduous crop species is older than the one that includes the tropical evergreen species, while incongruence between plastid and nuclear phylogenies suggests that the latter lineage originated via an ancient hybridization event. The most recent common ancestor (MRCA) of the temperate crop species was a component of the continuous boreotropical forests of the Northern Hemisphere, while the MRCA of the tropical species represented the last remains of the boreotropical elements and subsequently radiated throughout the Old and New World tropics from refugial areas at lower latitudes. Complex biogeographic histories leading to the present global distribution of the genus were driven by several geologic events

    11. Analysis of human induced changes in a karst landscape - the filling of dolines in the Kras plateau, Slovenia.

      Science.gov (United States)

      Kovačič, Gregor; Ravbar, Nataša

      2013-03-01

      A comprehensive analysis of the increased pressure on karst landscapes due to expansive economic and urban development is presented with the aim of evaluating changes in land use and their deleterious effects on karst relief forms. The study focuses on two areas surrounding the relatively quickly growing settlements of Hrpelje-Kozina and Divača on the Kras plateau (Slovenia) that have been subjected to intensive urban and business development and traffic since the motorway was brought to their vicinity fifteen years ago. National legislation loopholes and technological improvement were the cause of the commonly unsupervised human encroachment which caused the widespread degradation of the landscape. By comparing different topographical and ortophotographical materials from the past four decades and by detailed field inspection of land use and environmental changes, as well as the morphometrical characterization of dolines, the following results have been found: due to the population growth in the past four decades (39% and 50%, respectively), an increase of settlement area by 18 and 11 percentage points took place. Consequently, between 25 and 27% of dolines have disappeared or have been extensively modified (filled up and leveled). According to the local spatial plans, an additional 18% to 28% dolines are endangered. Broad human induced changes in the karst landscape have resulted in a noticeable increase in landscape deterioration, which is consistent with similar phenomena observed in other regions. Due to the extreme susceptibility of the karst to human activities that may lead to the degradation of its exceptional esthetic and environmental value, the alteration of karst processes such as corrosion, endangering of unique habitats and the quality of non-renewable natural resources, it is necessary to promptly define measures for its protection at the national level. Copyright © 2013 Elsevier B.V. All rights reserved.

    12. Molecular Biogeography of Tribe Thermopsideae (Leguminosae: A Madrean-Tethyan Disjunction Pattern with an African Origin of Core Genistoides

      Directory of Open Access Journals (Sweden)

      Ming-Li Zhang

      2015-01-01

      Full Text Available Thermopsideae has 45 species and exhibits a series of interesting biogeographical distribution patterns, such as Madrean-Tethyan disjunction and East Asia-North America disjunction, with a center of endemism in the Qinghai-Xizang Plateau (QTP and Central Asia. Phylogenetic analysis in this paper employed maximum likelihood using ITS, rps16, psbA-trnH, and trnL-F sequence data; biogeographical approaches included BEAST molecular dating and Bayesian dispersal and vicariance analysis (S-DIVA. The results indicate that the core genistoides most likely originated in Africa during the Eocene to Oligocene, ca. 55-30 Ma, and dispersed eastward to Central Asia at ca. 33.47 Ma. The origin of Thermopsideae is inferred as Central Asian and dated to ca. 28.81 Ma. Ammopiptanthus is revealed to be a relic. Birth of the ancestor of Thermopsideae coincided with shrinkage of the Paratethys Sea at ca. 30 Ma in the Oligocene. The Himalayan motion of QTP uplift of ca. 20 Ma most likely drove the diversification between Central Asia and North America. Divergences in East Asia, Central Asia, the Mediterranean, and so forth, within Eurasia, except for Ammopiptanthus, are shown to be dispersals from the QTP. The onset of adaptive radiation at the center of the tribe, with diversification of most species in Thermopsis and Piptanthus at ca. 4-0.85 Ma in Tibet and adjacent regions, seems to have resulted from intense northern QTP uplift during the latter Miocene to Pleistocene.

    13. Qualified temperature, salinity and dissolved oxygen climatologies in a changing Adriatic Sea

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      M. Lipizer

      2014-02-01

      Full Text Available An updated climatology, based on a comprehensive dataset (1911–2009 of temperature, salinity and dissolved oxygen, has been produced for the whole Adriatic Sea with the Variational Inverse Method using the DIVA software. Climatological maps were produced at 26 levels and validated with Ordinary Cross Validation and with real vs. synthetic Temperature–Salinity diagram intercomparison. The concept of Climatology–Observation Misfit (COM has been introduced as an estimate of the physical variability associated with the climatological structures. In order to verify the temporal stability of the climatology, long-term variability has been investigated in the Mid Adriatic and the South Adriatic Pits, regarded as the most suitable records of possible long-term changes. Compared with previous climatologies, this study reveals a surface temperature rise (up to 2 °C, a clear deep dissolved oxygen minimum in the South Adriatic Gyre and a bottom summer oxygen minimum in the North Adriatic. Below 100 m all properties profoundly differ between the Middle and the South Adriatic. The South Adriatic Pit clearly shows the remote effects of the Eastern Mediterranean Transient, while no effect is observed in Middle Adriatic Pits. The deepest part of the South Adriatic seems now to be significantly saltier (+0.18 since the period 1911–1914, with an increase of +0.018 decade−1 since the late 1940s and warmer (+0.54 °C since 1911–1914, even though a long-term temperature trend could not be statistically demonstrated. Conversely, the Middle Adriatic Pits present a long-term increase in apparent oxygen utilisation (+0.77 mL L−1 since 1911–1914, with a constant increase of +0.2 mL L−1 decade−1 after the 1970s.

    14. A well-sampled phylogenetic analysis of the polystichoid ferns (Dryopteridaceae) suggests a complex biogeographical history involving both boreotropical migrations and recent transoceanic dispersals.

      Science.gov (United States)

      Le Péchon, Timothée; Zhang, Liang; He, Hai; Zhou, Xin-Mao; Bytebier, Benny; Gao, Xin-Fen; Zhang, Li-Bing

      2016-05-01

      Intercontinental disjunctions in ferns have often been considered as the result of long-distance dispersal (LDD) events rather than of vicariance. However, in many leptosporangiate groups, both processes appear to have played a major role in shaping current geographical distribution. In this study, we reconstructed the phylogenetic relationships and inferred the ancestral distribution areas of the polystichoid ferns (Cyrtomium, Phanerophlebia, and Polystichum), to evaluate the relative impact of vicariance and LDD on the biogeography of this group. We used a molecular dataset including 3346 characters from five plastid loci. With 190 accessions our taxon coverage was about three times as large as any previous worldwide sampling. Biogeographical analyses were performed using S-DIVA and S-DEC and divergence times were estimated by integrating fossil and secondary calibrations. The polystichoid ferns are a monophyletic clade that may have originated in East Asia during the Eocene, an age much younger than previously estimated. Three transoceanic disjunctions between East Asia and New World were identified in the Paleogene: one for Phanerophlebia during late Eocene (34Ma, 19-51Ma), and two in Polystichum at the Eocene-Oligocene boundary (30Ma, 18-43Ma; 28Ma, 19-39Ma respectively). During the Neogene, further range expansions took place from Asia to Africa, Hawaii, and the Southwestern Indian Ocean region. Our results indicate that early transfers between the Old and the New World are compatible with a boreotropical migration scenario. After evolving in Asia during the Eocene, the polystichoid ferns reached the New World in independent migrations at the Eocene-Oligocene boundary through the boreotropical belt. However, although less likely, the alternative hypothesis of independent transoceanic dispersals from the Old to the New World cannot be ruled out. Further range expansion during the Neogene was most likely the result of long-distance dispersal (LDD).

    15. Phylogeny and historical biogeography of the lastreopsid ferns (Dryopteridaceae).

      Science.gov (United States)

      Labiak, Paulo H; Sundue, Michael; Rouhan, Germinal; Hanks, Judith G; Mickel, John T; Moran, Robbin C

      2014-07-01

      • Premise of the study: As currently circumscribed, Lastreopsis has about 45 species and occurs in Australia, southern Asia, Africa, Madagascar, and the neotropics. Previous molecular phylogenetic studies suggested that Lastreopsis is paraphyletic. Our study focuses on resolving relationships among the lastreopsid ferns (Lastreopsis, Megalastrum, and Rumohra), the evolution of morphological characters, and an understanding of the temporal and spatial patterns that have led to the current diversity and geographical distribution of its extant species.• Methods: Phylogenetic relationships were recovered under Bayesian, maximum likelihood, and maximum parsimony methods, using a data set of four plastid markers. Divergence time estimates were made using BEAST, and the biogeographic hypotheses were tested under the DEC model and the RASP/S-DIVA methods.• Key results: Lastreopsis was recovered as paraphyletic, and at least one of its clades should be recognized as a distinct genus, Parapolystichum. Coveniella poecilophlebia and Oenotrichia tripinnata were nested within Lastreopsis s.s., Megalastrum and Rumohra as sister to the Lastreopsis s.s., and the Lastreopsis amplissima clades. The initial diversification of the lastreopsids took place at about 56.55 Ma, from a neotropical ancestor.• Conclusions: Taxonomic recognition of Parapolystichum is warranted to preserve the monophyly of Lastreopsis. Diversification among the main clades of the lastreopsid ferns was influenced by climatic and geological changes in the southern hemisphere. The biogeographic history of the group is intimately related to the trans-Antarctic corridor between Australia and South America, with evidence for multiple lineage interchanges between Australia and South America during the Oligocene and the Eocene epochs.

    16. De epifania a método: a teopolítica do testemunho em um seminário pentecostal em Gana

      Directory of Open Access Journals (Sweden)

      Bruno Reinhardt

      Full Text Available Resumo Neste artigo, analiso a teopolítica do testemunho em um seminário Pentecostal baseado em Gana. Meu foco etnográfico é o testemunho do fundador da denominação Lighthouse Chapel International, que relata como ele recebeu a sua dádiva para ensinar através de uma impartição (transferência do carisma midiática. No seminário de sua denominação, essa inusitada epifania transforma-se em um verdadeiro método de impartição: a “saturação em fitas-cassete”. Como pode a experiência carismática, normalmente definida por sua inefabilidade, adquirir o poder de replicação típico dos métodos e das técnicas? Destaco como o problema da autenticação do testemunho é diretamente endereçado pela pedagogia desse seminário através de duas operações discursivas: um enquadramento metapragmático das relações de aprendizado ministerial via figuração bíblica e uma noção êmica de transmissão que articula carisma, mimetismo e repetição de maneira não contraditória. Concluo revisitando a natureza do poder que flui para e através desse líder carismático, tanto em seu testemunho quanto no método que é dele extraído.

    17. Vaccination as a tool to combat introductions of notifiable avian influenza viruses in Europe, 2000 to 2006.

      Science.gov (United States)

      Capua, I; Schmitz, A; Jestin, V; Koch, G; Marangon, S

      2009-04-01

      In late 2000, Italy was the first country of the European Union (EU) to implement an emergency vaccination programme against notifiable avian influenza. Vaccination with a conventional vaccine containing a seed strain with a different neuraminidase subtype from that of the field virus was used to complement biosecurity and restriction measures as part of an overall eradication strategy. This vaccination technique, in line with the Differentiating Infected from Vaccinated Animals system (DIVA), was applied several times until March 2008. This strategy enabled the identification of field exposed flocks and ultimately the eradication of low pathogenic H7N1, H7N3 and H5N2 infections. Italy was also the first country to implement a bivalent H5/H7 prophylactic vaccination programme of defined poultry populations, which was discontinued in December 2006. Following the incursion of highly pathogenic H5N1 into Europe, in 2005 and 2006, two other EU Member States, namely France and the Netherlands, implemented preventive vaccination programmes in 2006 but they targeted selected poultry populations different from those targeted in Italy and were implemented for short periods of time. Data generated during six years of experience with vaccination against avian influenza in Italy indicate that it is a useful tool to limit secondary spread and possibly prevent the introduction of low pathogenic avian influenza viruses in a susceptible population. The experience of France and the Netherlands provides data on vaccination of ducks and hobby poultry respectively and monitoring programmes associated with vaccination and difficulties related to their application. The advantages and disadvantages of vaccination need to be considered in the decision-making process, including the financial aspects of vaccination.

    18. Historical biogeography of the Angelica group (Apiaceae tribe Selineae) inferred from analyses of nrDNA and cpDNA sequences

      Institute of Scientific and Technical Information of China (English)

      Chen-Yang LIAO; Stephen R.DOWNIE; Yan YU; Xing-Jin HE

      2012-01-01

      Biogeographical patterns and diversification processes of Asia-centered angiosperm groups have been significantly affected by the multistage uplift of the Himalayas-Tibetan Plateau since the Late Tertiary.The divergence time of the largely East Asian Angelica group (Apiaceae,subfamily Apioideae,tribe Selineae) was initially analyzed using BEAST and nrDNA internal transcribed spacer sequence data from 96 representatives of tribe Selineae and relatives.Further analyses of the biogeographical history of the Angelica group were carried out using BEAST,S-DIVA,RASP,and LAGRANGE on datasets containing all or some of the following loci:nrDNA internal and external transcribed spacers; cpDNA rps16 intron; and cpDNA rps16-trnK,rpl32-trnL,and trnL-trnT intergenic spacers.The results suggested that the Angelica group was originally present in the East Palearctic during the global cooling of the late Middle Miocene (13.6 Mya) and that the Angelica s.s.clade originated in the same region at 10.2Mya.Subsequent diversifications of the Angelica s.s.clade intensified in the East Palearctic during the middle Late Miocene (10.0-7.0 Mya) and in the eastern Himalayan Zone during the late Pliocene and Pleistocene (<4.0Mya).These diversifications likely corresponded with plateau uplift-driven climatic changes.Considering elevational reconstructions,the differential responses to altitude appear to be the primary factor explaining the recent radiation of the group in the eastern Himalayas.The North American species of the Angelica group were retrieved as polyphyletic and their migrations involved six independent dispersals to North America at least since the middle Late Miocene,including four times from northeast Asia and twice from Europe.

    19. Phylogeny and biogeography of exacum (gentianaceae): a disjunctive distribution in the Indian ocean basin resulted from long distance dispersal and extensive radiation.

      Science.gov (United States)

      Yuan, Yong-Ming; Wohlhauser, Sébastien; Möller, Michael; Klackenberg, Jens; Callmander, Martin; Küpfer, Philippe

      2005-02-01

      Disjunctive distributions across paleotropical regions in the Indian Ocean Basin (IOB) often invoke dispersal/vicariance debates. Exacum (Gentianaceae, tribe Exaceae) species are spread around the IOB, in Africa, Madagascar, Socotra, the Arabian peninsula, Sri Lanka, India, the Himalayas, mainland Southeast Asia including southern China and Malaysia, and northern Australia. The distribution of this genus was suggested to be a typical example of vicariance resulting from the breakup of the Gondwanan supercontinent. The molecular phylogeny of Exacum is in principle congruent with morphological conclusions and shows a pattern that resembles a vicariance scenario with rapid divergence among lineages, but our molecular dating analysis demonstrates that the radiation is too recent to be associated with the Gondwanan continental breakup. We used our dating analysis to test the results of DIVA and found that the program predicted impossible vicariance events. Ancestral area reconstruction suggests that Exacum originated in Madagascar, and divergence dating suggests its origin was not before the Eocene. The Madagascan progenitor, the most recent common ancestor of Exacum, colonized Sri Lanka and southern India via long-distance dispersals. This colonizer underwent an extensive range expansion and spread to Socotra-Arabia, northern India, and mainland Southeast Asia in the northern IOB when it was warm and humid in these regions. This widespread common ancestor retreated subsequently from most parts of these regions and survived in isolation in Socotra-Arabia, southern India-Sri Lanka, and perhaps mainland Southeast Asia, possibly as a consequence of drastic climatic changes, particularly the spreading drought during the Neogene. Secondary diversification from these surviving centers and Madagascar resulted in the extant main lineages of the genus. The vicariance-like pattern shown by the phylogeny appears to have resulted from long-distance dispersals followed by extensive

    20. The comorbidity of anxiety and depressive symptoms in older adults with attention-deficit/hyperactivity disorder: a longitudinal study.

      Science.gov (United States)

      Michielsen, Marieke; Comijs, Hannie C; Semeijn, Evert J; Beekman, Aartjan T F; Deeg, Dorly J H; Sandra Kooij, J J

      2013-06-01

      Comorbidity between Attention-Deficit/Hyperactivity Disorder (ADHD) and depression and anxiety disorders in children and young to middle-aged adults has been well documented in the literature. Yet, it is still unknown whether this comorbidity persists into later life. The aim of this study is therefore to examine the comorbidity of anxiety and depressive symptoms among older adults with ADHD. This is examined both using cross-sectional and longitudinal data. Data were used from the Longitudinal Aging Study Amsterdam (LASA). Participants were examined in three measurement cycles, covering six years. They were asked about depressive and anxiety symptoms. To diagnose ADHD, the DIVA 2.0, a diagnostic interview was administered among a subsample (N=231, age 60-94). In addition to the ADHD diagnosis, the association between the sum score of ADHD symptoms and anxiety and depressive symptoms was examined. Data were analyzed by means of linear regression analyses and linear mixed models. Both ADHD diagnosis and more ADHD symptoms were associated with more anxiety and depressive symptoms cross-sectionally as well as longitudinally. The longitudinal analyses showed that respondents with higher scores of ADHD symptoms reported an increase of depressive symptoms over six years whereas respondents with fewer ADHD symptoms remained stable. The ADHD diagnosis is based on the DSM-IVcriteria, which were developed for children, and have not yet been validated in (older) adults. It appears that the association between ADHD and anxiety/depression remains in place with aging. This suggests that, in clinical practice, directing attention to both in concert may be fruitful. Copyright © 2012 Elsevier B.V. All rights reserved.

    1. Multiple linear B-cell epitopes of classical swine fever virus glycoprotein E2 expressed in E.coli as multiple epitope vaccine induces a protective immune response

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      Wei Jian-Chao

      2011-07-01

      Full Text Available Abstract Classical swine fever is a highly contagious disease of swine caused by classical swine fever virus, an OIE list A pathogen. Epitope-based vaccines is one of the current focuses in the development of new vaccines against classical swine fever virus (CSFV. Two B-cell linear epitopes rE2-ba from the E2 glycoprotein of CSFV, rE2-a (CFRREKPFPHRMDCVTTTVENED, aa844-865 and rE2-b (CKEDYRYAISSTNEIGLLGAGGLT, aa693-716, were constructed and heterologously expressed in Escherichia coli as multiple epitope vaccine. Fifteen 6-week-old specified-pathogen-free (SPF piglets were intramuscularly immunized with epitopes twice at 2-week intervals. All epitope-vaccinated pigs could mount an anamnestic response after booster vaccination with neutralizing antibody titers ranging from 1:16 to 1:256. At this time, the pigs were subjected to challenge infection with a dose of 1 × 106 TCID50 virulent CSFV strain. After challenge infection, all of the rE2-ba-immunized pigs were alive and without symptoms or signs of CSF. In contrast, the control pigs continuously exhibited signs of CSF and had to be euthanized because of severe clinical symptoms at 5 days post challenge infection. The data from in vivo experiments shown that the multiple epitope rE2-ba shown a greater protection (similar to that of HCLV vaccine than that of mono-epitope peptide(rE2-a or rE2-b. Therefore, The results demonstrated that this multiple epitope peptide expressed in a prokaryotic system can be used as a potential DIVA (differentiating infected from vaccinated animals vaccine. The E.coli-expressed E2 multiple B-cell linear epitopes retains correct immunogenicity and is able to induce a protective immune response against CSFV infection.

    2. “Telhado de Vidro” nas ONGs: Enfrentamento da Questão Social e Desafios Ao Serviço Social

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      Janaína Lopes do Nascimento

      2012-02-01

      Full Text Available <div>A dinâmica societária contemporânea tem alterado as relações entre Estado e Sociedade, com sérias implicações à garantia de direitos sociais conquistados pela classe trabalhadora, a partir do movimento de precarização

    e privatização das políticas sociais. No Brasil, após 1990, as ONGs, inseridas na lógica contraditória do “terceiro setor”, tornam-se uma das protagonistas no processo da contrarreforma do Estado, caracterizando a fragilidade política
    e social, ou melhor, o “telhado de vidro” no enfrentamento da questão social. É diante dessa conjuntura que o presente artigo objetiva problematizar alguns desafios postos aos Assistentes Sociais nas ONGs. Ressalta-se que é no conjunto das determinações sócio-históricas atuais que se constituem e reconstituem limites e possibilidades de atuação em tempos de crise do capital; dentre os desafios, enfatiza-se o atendimento de demandas  versus  as necessidades sociais
    dos usuários e a relação de “parceria” que se estabelece entre ONGs e Estado no âmbito da questão social

  • Comparison of morphine, ketoprofen and Arnica montana 6x and 30x per oral transmucosal or subcutaneous route for control of postoperative pain in cats subjected to hysterectomy with bilateral salpingo-oophorectomy

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    Denise de Fátima Rodrigues

    2016-02-01

    Full Text Available ABSTRACT: The postoperative analgesic effect of Arnica montana (Arnica was compared to morphine and ketoprofen in 50 cats following hysterectomy with bilateral salpingo-oophorectomy (HSO. Cats were randomly allocated to five groups (n=10 and were treated 30 minutes before surgery and over 72 hours with 1ml of Arnica 30x per subcutaneous (SC route (GA30SC; Arnica 30x per oral transmucosal route (P.O. (GA30PO; Arnica 6x P.O. (GA6PO; morphine 0.1mg kg-1 SC (GM SID or ketoprofen 2mg kg-1 SC (GK before and 1mg kg-1 after surgery. Sedation and postoperative pain were assessed by means of a dynamic and interactive visual analogue scale (DIVAS and variable count scale (VCS and hyperalgesia using an esthesiometer. Whenever the VAS and VCS scores attained 33% of the maximum value, rescue analgesia was performed with morphine 0.3mg kg-1 per the intramuscular (IM route. Other variables assessed were vomiting, defecation, urination, body weight and wound healing. Vomiting only occurred in animals treated with morphine. The groups did not differ in defecation, urination, body weight or wound healing. Hyperalgesia was detected only on the occasions that the criterion for rescue analgesia was met. One animal in GK and one in GM required rescue analgesia, differing from groups GA30SC, GA30PO and GA6PO, in which 4, 4 and 5 animals, respectively, required rescue analgesia. It can be concluded that ketoprofen and morphine were more efficacious than Arnica and that there was no difference among the various potencies and routes of administration of Arnica for postoperative analgesia in cats undergoing HSO.

  • LCLUC as an entry point for transdisciplinary research--reflections from an agriculture land use change study in South Asia.

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    Nagabhatla, Nidhi; Padmanabhan, Martina; Kühle, Peter; Vishnudas, Suma; Betz, Lydia; Niemeyer, Bastian

    2015-01-15

    This article highlights applied understanding of classifying earth imaging data for land cover land use change (LCLUC) information. Compared to the many previous studies of LCLUC, the present study is innovative in that it applied geospatial data, tools and techniques for transdisciplinary research. It contributes to a wider discourse on practical decision making for multi-level governance. Undertaken as part of the BioDIVA project, the research adopted a multi-tiered methodical approach across three key dimensions: socioecology as the sphere of interest, a transdisciplinary approach as the disciplinary framework, and geospatial analysis as the applied methodology. The area of interest was the agroecosystem of Wayanad district in Kerala, India (South Asia). The methodology was structured to enable analysis of multi-scalar and multi-temporal data, using Wayanad as a case study. Three levels of analysis included: District (Landsat TM-30m), Taluk or sub-district (ASTER-15m) and Village or Gram Panchayat (GeoEye-0.5m). Our hypothesis, that analyzing patterns of land use change is pertinent for up-to-date assessment of agroecosystem resources and their wise management is supported by the outcome of the multi-tiered geospatial analysis. In addition, two examples from the project that highlight the adoption of LCLUC by different disciplinary experts are presented. A sociologist assessed the land ownership boundary for a selected tribal community. A faunal ecologist used it to assess the effect of landscape structure on arthropods and plant groups in rice fields. Furthermore, the Google Earth interface was used to support the overall validation process. Our key conclusion was that a multi-level understanding of the causes, effects, processes and mechanisms that govern agroecosystem transformation requires close attention to spatial, temporal and seasonal dynamics, for which the incorporation of local knowledge and participation of local communities is crucial. Copyright

  • Correlation of histological findings from a large ciliochoroidal melanoma with CT perfusion and 3T MRI dynamic enhancement studies

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    Jose S Pulido

    2008-06-01

    Full Text Available Jose S Pulido1, Norbert G Campeau2, Ernst Klotz3, Andrew N Primak2, Osama Saba3, Kaan Gunduz1, Herbert Cantrill5, Diva Salomão1,4, Cynthia H McCollough21Department of Ophthalmology; 2Department of Radiology; 3Siemens Medical Solutions, Malvern, PA, USA; 4Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA; 5VitreoRetinal Surgery, Minneapolis, MN, USABackground: The initial use of a 64-slice computed tomography (CT scanner for obtaining quantitative perfusion data from a large ciliochoroidal melanoma, and correlation with 3T magnetic resonance imaging (MRI dynamic enhancement and tumor histology.Methods: The CT perfusion scan was performed using 80 kVp, 250 mA and 1-sec rotation time for 40 sec. The analysis was performed using commercial perfusion analysis software with a prototype 3-dimensional motion correction tool. Dynamic contrast-enhanced 3-Tesla MRI measured the kinetics of enhancement to estimate the vascular permeability. The time-dependent enhancement patterns were obtained using the average signal intensity using Functool analysis software. The involved globe was enucleated and microscopic evaluation of the tumor was performed.Results: The perfusion parameters blood flow, blood volume and permeability surface area product in the affected eye determined by CT perfusion analysis were 118 ml/100 ml/min, 11.3 ml/100 ml and 48 ml/100 ml/min. Dynamic MRI enhancement showed maximal intensity increase of 111%. The neoplasm was a ciliochoroidal spindle cell melanoma which was mitotically active (13 mitoses/40 hpf. Vascular loops and arcades were present throughout the tumor. The patient developed metastases within 9 months of presentation.Conclusion: Quantitative CT perfusion analysis of ocular tumors is feasible with motion correction software.Keywords: ciliochoroidal melanoma, CT perfusion imaging, MR enhancement imaging, tumor blood volume, tumor blood flow, tumor permeability

  • A novel recombinant Peste des petits ruminants-canine adenovirus vaccine elicits long-lasting neutralizing antibody response against PPR in goats.

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    Junling Qin

    Full Text Available BACKGROUND: Peste des petits ruminants (PPR is a highly contagious infectious disease of goats, sheep and small wild ruminant species with high morbidity and mortality rates. The Peste des petits ruminants virus (PPRV expresses a hemagglutinin (H glycoprotein on its outer envelope that is crucial for viral attachment to host cells and represents a key antigen for inducing the host immune response. METHODOLOGY/PRINCIPAL FINDINGS: To determine whether H can be exploited to generate an effective PPRV vaccine, a replication-competent recombinant canine adenovirus type-2 (CAV-2 expressing the H gene of PPRV (China/Tibet strain was constructed by the in vitro ligation method. The H expression cassette, including the human cytomegalovirus (hCMV promoter/enhancer and the BGH early mRNA polyadenylation signal, was inserted into the SspI site of the E3 region, which is not essential for proliferation of CAV-2. Infectious recombinant rCAV-2-PPRV-H virus was generated in transfected MDCK cells and used to immunize goats. All vaccinated animals produced antibodies upon primary injection that were effective in neutralizing PPRV in vitro. Higher antibody titer was obtained following booster inoculation, and the antibody was detectable in goats for at least seven months. No serious recombinant virus-related adverse effect was observed in immunized animals and no adenovirus could be isolated from the urine or feces of vaccinated animals. Results showed that the recombinant virus was safe and could stimulate a long-lasting immune response in goats. CONCLUSIONS/SIGNIFICANCE: This strategy not only provides an effective PPR vaccine candidate for goats but may be a valuable mean by which to differentiate infected from vaccinated animals (the so-called DIVA approach.

  • Peste des Petits Ruminants, the next eradicated animal disease?

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    Albina, Emmanuel; Kwiatek, Olivier; Minet, Cécile; Lancelot, Renaud; Servan de Almeida, Renata; Libeau, Geneviève

    2013-07-26

    Peste des Petits Ruminants (PPR) is a widespread viral disease caused by a Morbillivirus (Paramyxoviridae). There is a single serotype of PPR virus, but four distinct genetic lineages. Morbidity and mortality are high when occurring in naive sheep and goats populations. Cattle and African buffaloes (Syncerus caffer) are asymptomatically infected. Other wild ruminants and camels may express clinical signs and mortality. PPR has recently spread in southern and northern Africa, and in central and far-east Asia. More than one billion sheep and goats worldwide are at risk. PPR is also present in Europe through western Turkey. Because of its clinical incidence and the restrictions on animal movements, PPR is a disease of major economic importance. A live attenuated vaccine was developed in the 1980s, and has been widely used in sheep and goats. Current researches aim (i) to make it more thermotolerant for use in countries with limited cold chain, and (ii) to add a DIVA mark to shorten and reduce the cost of final eradication. Rinderpest virus-another Morbillivirus-was the first animal virus to be eradicated from Earth. PPRV has been proposed as the next candidate. Considering its wide distribution and its multiple target host species which have an intense mobility, it will be a long process that cannot exclusively rely on mass vaccination. PPR specific epidemiological features and socio-economic considerations will also have to be taken into account, and sustained international, coordinated, and funded strategy based on a regional approach of PPR control will be the guarantee toward success. Copyright © 2012 Elsevier B.V. All rights reserved.

  • Mutations in the Lactococcus lactis Ll.LtrB group II intron that retain mobility in vivo

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    D'Souza Lisa M

    2002-12-01

    Full Text Available Abstract Background Group II introns are mobile genetic elements that form conserved secondary and tertiary structures. In order to determine which of the conserved structural elements are required for mobility, a series of domain and sub-domain deletions were made in the Lactococcus lactis group II intron (Ll.LtrB and tested for mobility in a genetic assay. Point mutations in domains V and VI were also tested. Results The largest deletion that could be made without severely compromising mobility was 158 nucleotides in DIVb(1–2. This mutant had a mobility frequency comparable to the wild-type Ll.LtrB intron (ΔORF construct. Hence, all subsequent mutations were done in this mutant background. Deletion of DIIb reduced mobility to approximately 18% of wild-type, while another deletion in domain II (nts 404–459 was mobile to a minor extent. Only two deletions in DI and none in DIII were tolerated. Some mobility was also observed for a DIVa deletion mutant. Of the three point mutants at position G3 in DV, only G3A retained mobility. In DVI, deletion of the branch-point nucleotide abolished mobility, but the presence of any nucleotide at the branch-point position restored mobility to some extent. Conclusions The smallest intron capable of efficient retrohoming was 725 nucleotides, comprising the DIVb(1–2 and DII(iia,b deletions. The tertiary elements found to be nonessential for mobility were alpha, kappa and eta. In DV, only the G3A mutant was mobile. A branch-point residue is required for intron mobility.

  • Immunity, safety and protection of an Adenovirus 5 prime--Modified Vaccinia virus Ankara boost subunit vaccine against Mycobacterium avium subspecies paratuberculosis infection in calves.

    Science.gov (United States)

    Bull, Tim J; Vrettou, Christina; Linedale, Richard; McGuinnes, Catherine; Strain, Sam; McNair, Jim; Gilbert, Sarah C; Hope, Jayne C

    2014-10-29

    Vaccination is the most cost effective control measure for Johne's disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) but currently available whole cell killed formulations have limited efficacy and are incompatible with the diagnosis of bovine tuberculosis by tuberculin skin test. We have evaluated the utility of a viral delivery regimen of non-replicative human Adenovirus 5 and Modified Vaccinia virus Ankara recombinant for early entry MAP specific antigens (HAV) to show protection against challenge in a calf model and extensively screened for differential immunological markers associated with protection. We have shown that HAV vaccination was well tolerated, could be detected using a differentiation of infected and vaccinated animals (DIVA) test, showed no cross-reactivity with tuberculin and provided a degree of protection against challenge evidenced by a lack of faecal shedding in vaccinated animals that persisted throughout the 7 month infection period. Calves given HAV vaccination had significant priming and boosting of MAP derived antigen (PPD-J) specific CD4+, CD8+ IFN-γ producing T-cell populations and, upon challenge, developed early specific Th17 related immune responses, enhanced IFN-γ responses and retained a high MAP killing capacity in blood. During later phases post MAP challenge, PPD-J antigen specific IFN-γ and Th17 responses in HAV vaccinated animals corresponded with improvements in peripheral bacteraemia. By contrast a lack of IFN-γ, induction of FoxP3+ T cells and increased IL-1β and IL-10 secretion were indicative of progressive infection in Sham vaccinated animals. We conclude that HAV vaccination shows excellent promise as a new tool for improving control of MAP infection in cattle.

  • Detecting land-cover change using mappable vegetation related indices: A case study from Sinharaja Man and the Biosphere Reserve

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    BD Madurapperuma

    2014-06-01

    Full Text Available This study evaluates multi-year changes of vegetation in the Sinharaja Man and the Biosphere (MAB reserve using mappable vegetation related indices viz., Normalized Difference Vegetation Index (NDVI and Burn Index (BI. Land-cover changes in the Sinharaja MAB reserve were detected using Landsat 7 ETM+ images for 1993, 2001, and 2005. Seven individual bands of each image were converted to new multiband files by layer stacking using ENVI® 4.5. Then the multiband files were re-projected to UTM Zone 44 North, WGS-84 Datum. Each data set was exported to ENVI® EX software package to detect the changes between time steps based on NDVI and BI using an image difference tool. Land-cover data, which were obtained from the DIVA GIS web portal, were compared with Landsat image data. Results of BI showed that the Sinharaja MAB reserve fringe was vulnerable to forest fire. For example, from 1993- 2001, 160 ha identified as burned area. In contrast, from 2001-2005, 79 ha burned, and for the entire period of 1993-2005, 10 ha burned. NDVI resulted in a 962 ha increase of vegetation prime at the western Sinharaja from 2001-2005. In addition, there was a 15 ha decrease in vegetation from 1993-2005. The results were visualized using an embedded 3D render window of Google Earth and 2D view of ArcGIS explorer online. In conclusion, in-situ ground truthing data is needed for the fire-influenced area for implementing sustainable forest resource management at the Sinharaja MAB reserve. Normal 0 false false false EN-GB X-NONE X-NONE

  • Using 13C-labeled benzene and Raman gas spectroscopy to investigate respiration and biodegradation kinetics following soil contamination

    Science.gov (United States)

    Jochum, Tobias; Popp, Juergen; Frosch, Torsten

    2016-04-01

    Soil and groundwater contamination with benzene can cause serious environmental damages. However, many soil microorganisms are capable to adapt and known to strongly control the fate of organic contamination. Cavity enhanced Raman gas spectroscopy (CERS) was applied to investigate the short-term response of indigenous soil bacteria to a sudden surface contamination with benzene regarding the temporal variations of gas products and their exchange rates with the adjacent atmosphere. 13C-labeled benzene was spiked on a silty-loamy soil column (sampled from Hainich National Park, Germany) in order to track and separate the changes in heterotrophic soil respiration - involving 12CO2 and O2 - from the microbial process of benzene degradation, which ultimately forms 13CO2.1 The respiratory quotient (RQ) of 0.98 decreased significantly after the spiking and increased again within 33 hours to a value of 0.72. This coincided with maximum 13CO2 concentration rates (0.63 μ mol m-2 s-1), indicating highest benzene degradation at 33 hours after the spiking event. The diffusion of benzene in the headspace and the biodegradation into 13CO2 were simultaneously monitored and 12 days after the benzene spiking no measurable degradation was detected anymore.1 The RQ finally returned to a value of 0.96 demonstrating the reestablished aerobic respiration. In summary, this study shows the potential of combining Raman gas spectroscopy and stable isotopes to follow soil microbial biodegradation dynamics while simultaneously monitoring the underlying respiration behavior. Support by the Collaborative Research Center 1076 Aqua Diva is kindly acknowledged. We thank Beate Michalzik for soil analysis and discussion. 1. T. Jochum, B. Michalzik, A. Bachmann, J. Popp and T. Frosch, Analyst, 2015, 140, 3143-3149.

  • Molecular phylogeny and biogeography of Picea (Pinaceae): implications for phylogeographical studies using cytoplasmic haplotypes.

    Science.gov (United States)

    Ran, Jin-Hua; Wei, Xiao-Xin; Wang, Xiao-Quan

    2006-11-01

    The center of diversity is not necessarily the place of origin, as has been established by many plant molecular phylogenies. Picea is a complicated but very important genus in coniferous forests of the Northern Hemisphere, with a high species diversity in Asia. Its phylogeny and biogeography were investigated here using sequence analysis of the paternally inherited chloroplast trnC-trnD and trnT-trnF regions and the maternally inherited mitochondrial nad5 intron 1. We found that the North American P. breweriana and P. sitchensis were basal to the other spruces that were further divided into three clades in the cpDNA phylogeny, and that the New World species harbored four of five mitotypes detected, including two ancestral ones and three endemics. These results, combined with biogeographic analyses using DIVA and MacClade and fossil evidence, suggest that Picea originated in North America, and that its present distribution could stem from two times of dispersal from North America to Asia by the Beringian land bridge, and then from Asia to Europe. Most of the northeastern Asian species and the European P. abies could arise from a recent radiation given the very low interspecific genetic differentiation and pure mitotype of them. Considering that the ancestral mtDNA polymorphism can be preserved in many descendant species, even distantly related ones, we suggest that more species, at least the closely related ones, should be sampled in the phylogeographical study using cytoplasmic haplotypes if possible. In addition, we also discussed the evolution and phylogenetic utility of morphological characters in Picea.

  • Phylogeographic History of Atraphaxis Plants in Arid Northern China and the Origin of A. bracteata in the Loess Plateau

    Science.gov (United States)

    Xu, Zhe; Zhang, Ming-Li; Cohen, James I.

    2016-01-01

    In China, species of Atraphaxis (Polygonaceae) primarily inhabit arid zones across temperate steppe and desert regions. The complex geologic history (e.g., expansion of deserts) and extreme climate shifts of the region appear to have played an important role in shaping the phylogeography of Atraphaxis. The present study focuses on species-level phylogeographic patterns of Atraphaxis in China, with the goal of determining the impact of past environmental changes, in northern China, on the evolutionary history of the genus. Five hundred and sixty-four individuals distributed among 71 populations of 11 species of Atraphaxis from across the geographic range of the genus were studied using sequence data from two plastid spacers, psbK-psbI and psbB-psbH. The results demonstrate that most chloroplast haplotypes are species-specific, except for some present among widespread species. The phylogeny of Atraphaxis was well structured, and molecular dating analyses suggest that the main divergence events occurred during the late Pliocene and Pleistocene (5.73–0.03 million years ago). The statistical dispersal-vicariance analysis (S-DIVA) results provide evidence that phylogeographic patterns for the genus were characterized by both vicariance events and regional dispersal. The presented data suggest that the rapid expansion of deserts and climatic changes in northern China during the late Pliocene and Pleistocene have driven the diversification and spread of Atraphaxis in the region. The expansion of the Tengger Desert provided appropriate conditions for the origin of A. bracteata. Additionally, a contact zone in the north of the Hexi Corridor was identified as having played a significant role as a migratory route for species in adjacent areas. PMID:27656885

  • The use of incomplete global data for probabilistic event trees: challenges and strategies

    Science.gov (United States)

    Ogburn, Sarah; Harpel, Chris; Pesicek, Jeremy; Wellik, Jay; Wright, Heather; Pallister, John

    2016-04-01

    To prevent volcanic crises from becoming disasters, the USGS-USAID Volcano Disaster Assistance Program (VDAP) helps foreign counterparts to assess volcanic unrest, activity, and hazards before and during crises. Bayesian event trees are frequently used to facilitate discussion, reach consensus, evaluate uncertainty, and produce probabilistic forecasts of volcanic activity. VDAP uses a "method of multiple data sets" (Newhall & Pallister 2014), which combines conceptual and physical models of volcanic processes, current monitoring data, patterns of prior occurrence, and expert judgement from multiple disciplines to assign probabilities for each node of an event tree. The global volcanic record is used to inform our conceptual models, improve uncertainty estimates by leveraging larger datasets, and to fill in gaps where local information is sparse. For example, event trees for the recent Sinabung, Indonesia eruption relied upon local monitoring data-streams, but also on the global frequency-magnitude (VEI) of eruptions. A variety of databases are used, including the Smithsonian Institution's Global Volcanism Program (GVP) database, WOVOdat, GeoDIVA, DomeHaz, and FlowDat. Inhomogeneity and incompleteness of the global record present challenges for the use of such data in event trees, resulting in large and difficult to quantify uncertainties. Under-recording of small events, lack of documentation of 'failed eruptions', and variability of geophysical monitoring data-streams present particular problems. This contribution seeks to: (1) review VDAP's use of global data for probabilistic event tree creation; (2) summarize the problems presented by under-recording, spatial and temporal inhomogeneity, and incompleteness of the global record; (3) highlight ways to compensate for these effects, such as the development of hierarchical models to borrow strength from the global record while retaining local information, and the use of ranges in expert judgements to assess

  • Tracing spatial variation of canopy water fluxes to the soil: An experimental approach to assessing heterogeneity with high resolution data

    Science.gov (United States)

    Metzger, Johanna Clara; Dalla Valle, Nicolas; Wutzler, Thomas; Filipzik, Janett; Weckmüller, Josef; Schelhorn, Danny; Grauer, Christoph; Hildebrandt, Anke

    2016-04-01

    Due to the mechanisms of interception, stemflow and canopy throughfall, precipitation reaches a forest soil surface in an altered temporal and spatial distribution. The retention of water by canopies is contrasted by the formation of dynamic hotspots, which channel rain water down to the soil, e.g. canopy dripping points and stemflow. Throughfall patterns are often assumed to be the main driver for soil water content heterogeneity in forests, yet this relation has not been studied extensively and there is no prove to this theory. Because it is impossible to measure net precipitation input and soil water content in the same place at the same time, elaborate sampling is necessary for a data set allowing to address this subject. Within the last two years, in the framework of the Collaborative Research Centre AquaDiva, our group has established a new experimental site to investigate on the relationship of forest precipitation and soil water. We have developed an experimental design combining the measurement of throughfall, stemflow and soil water content on a 1 ha plot in a manner so all measurements are representative for the plot yet do not disturb each other. We adapted a statistical sampling method common for throughfall measurement to soil water content measurement comprising a high number of measurement points (throughfall: 350, soil water content: 210). To gain good insights in soil water dynamics and to capture preferential flow, soil water content was recorded in a high frequency (1* 6-1 min-1) in two soil depths. Stemflow was measured area-based on regular subplots (11% of the total plot area, 65 trees). Stand and soil surveys were made to investigate canopy impacts and identify soil-born patterns in soil water content. The plot is located in a mixed beech forest of the Hainich national park in Thuringia, Germany. Shallow loamy soils cover the lime- and marlstone bedrock. The forest plot is complemented by a smaller neighboring grassland plot acting as a

  • Caracterização parcial de um Tymovirus isolado de tomateiros

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    Isabel Cristina Batista

    2012-01-01

    Full Text Available Um Tymovirus isolado de sementes de tomateiro (Solanum lycopersicum 'Paronset' com sintomas de necrose, denominado TyToRS08, foi caracterizado quanto ao círculo de hospedeiras, propriedades físico-químicas, sorológicas e moleculares. O vírus foi transmitido mecanicamente a partir de folhas, frutos e sementes infectadas. A gama de hospedeiras incluiu espécies de Amaranthaceae, Chenopodiaceae e Solanaceae, induzindo sintomas local e sistêmicos na maior parte das espécies, exceto para Nicotiana sylvestris, N. tabacum 'Samsun', 'White Burley' e 'Xanthi' que não manifestaram sintomas. Em tomateiros 'Alambra', 'Thomas', 'Diva', 'Romana' e 'Graziela', o vírus induziu sintomas de mosaico e necrose branca. A presença do vírus foi detectada, nas sementes infectadas de todas as cultivares avaliadas, porém não se notou em plântulas após a germinação destas sementes. Foi obtida uma preparação purificada com concentração de 1,04 mg mL-1, empregada na produção de um antissoro policlonal que reagiu em PTA-ELISA com um título de 1/32768. Utilizou-se um par de oligonucleotídeos degenerados, desenhados para anelar na ORF 1 de espécies de Tymovirus, que permitiu a amplificação por RT-PCR de fragmentos com cerca de 700 pb. Após o alinhamento e a análise das sequências, verificou-se que o isolado TyToRS08 possuía valores abaixo de 70% de identidade com as espécies de Tymovirus, indicando que pode se tratar de uma espécie distinta das descritas no gênero, com potencial epidemiológico, pela sua estabilidade e disseminação por sementes de tomate.

  • Resenha: GOTTSCHALL, Jonathan. The story telling animal: how stories make us human. Boston,New York: Houghton Mifflin Harcourt, 2012. p. 248.

    Directory of Open Access Journals (Sweden)

    Marcus Assis Lima

    2014-04-01

    Full Text Available O autor é professor de Literatura Americana na Washington & Jefferson College, na Pensilvânia. Esse seu mais recente trabalho procura responder uma pergunta básica: Contar uma estória é apenas uma diversão e um passatempo ou tem alguma função biológica e, portanto, alguma função para espécie humana? Ele parte do princípio que "somos, como espécie, viciados em narrativas. Mesmo quando o corpo vai dormir, a mente continua alerta durante todo o sono, contando estórias a ela mesma" (p.xiv. Gottschall afirma que a mente humana não apenas foi "moldada para a ficção, mas que ela foi moldada pela ficção" (p.56. A "mente narrativa" é viciada em significados. Se ela não consegue encontrar um padrão de significados no mundo, ela irá tentar impor um padrão, ela é como uma fábrica que descarta muitas narrativas verdadeiras quando pode, mas que "também pode fabricar mentiras quando não encontra verdades" (p.103. Ele defende que uma estória de vida seria uma "mitologia pessoal" sobre quem somos - de onde viemos, como chegamos onde chegamos; são quem somos e formam nossa identidade. Em sua linha evolucionista de argumentos, Gottschall acredita que as narrativas, como os organismos biológicos, também evoluem, adaptando-se de acordo com demandas do ambiente. De modo que os seres humanos teriam evoluído para desejarem fortemente as narrativas e esse desejo tem sido um enorme benefício para todos nós: "As narrativas nos dão prazer e instruções. Elas simulam mundos de modo que possamos viver melhor no mundo reeal. Elas nos ajudam a criar laços comunais e a definir nossa cultura. As narrativas foram uma dádiva para nossa espécie" (p.197.

  • Charging of the Electric Vehicles in Private Sector: Technical and Economic Aspects

    Science.gov (United States)

    Grackova, L.; Oleinikova, I.; Klavs, G.

    2014-12-01

    The economic aspect considered in the work is related to the charging of an electric vehicle (EV) at a single private house when this is done every day. To perform the relevant cost estimation it was necessary to determine: the average annual electricity consumption under the condition of everyday charging an EV and the average electricity consumption off the mains for covering a distance of 100 km by an EV and the time in hours for its charging. Comparison is made for the day-time intervals from which it is possible to choose the preferable for proper loading the electric line and the most beneficial for the consumer. Under analysis are two EV connection scenarios for 100 individual households from which 10%, 20% and 30% have EVs, with 8-h duration of each charging at the current of 13A. The authors consider the consumption and electric energy payment packages which - with planned opening of the electric energy market on January 1, 2015 - will offer the clients the enterprises rendering services on the electric energy sale. Šajā rakstā tiek analizēts vidējais diennakts elektroenerģijas patēriņš un diennakts slodzes grafiki privātmājās apstākļos, kad privātmājas īpašnieki lādē elektroautomobili katru dienu vidējam brauciena attālumam 100 km. Elektroautomobiļa uzlādes režīmi tiek analizēti, ņemot vērā patēriņa elektriskās slodzes grafika kvalitāti raksturojošos rādītājus un patērētāja ekonomiskos ieguvumus. Lai novērtētu ekonomiski stimulētu patērētāju elektroautomobiļa uzlādes laika izvēles ietekmi uz diennakts elektriskās slodzes aizpildījuma koeficientu, tika izveidoti un analizēti 2 scenāriji, kas apraksta 100 privātmāju ar dažādu elektroautomobiļu piederības īpatsvaru divas atšķirīgas automobiļu uzlādes gadījumus.

  • Development of a food-exchange model to replace saturated fat with MUFAs and n-6 PUFAs in adults at moderate cardiovascular risk.

    Science.gov (United States)

    Weech, Michelle; Vafeiadou, Katerina; Hasaj, Marinela; Todd, Susan; Yaqoob, Parveen; Jackson, Kim G; Lovegrove, Julie A

    2014-06-01

    The recommendation to reduce saturated fatty acid (SFA) consumption to ≤10% of total energy (%TE) is a key public health target aimed at lowering cardiovascular disease (CVD) risk. Replacement of SFA with unsaturated fats may provide greater benefit than replacement with carbohydrates, yet the optimal type of fat is unclear. The aim of the DIVAS (Dietary Intervention and Vascular Function) study was to develop a flexible food-exchange model to investigate the effects of substituting SFAs with monounsaturated fatty acids (MUFAs) or n-6 (ω-6) polyunsaturated fatty acids (PUFAs) on CVD risk factors. In this parallel study, UK adults aged 21-60 y with moderate CVD risk (50% greater than the population mean) were identified using a risk assessment tool (n = 195; 56% females). Three 16-wk isoenergetic diets of specific fatty acid (FA) composition (%TE SFA:%TE MUFA:%TE n-6 PUFA) were designed using spreads, oils, dairy products, and snacks as follows: 1) SFA-rich diet (17:11:4; n = 65); 2) MUFA-rich diet (9:19:4; n = 64); and 3) n-6 PUFA-rich diet (9:13:10; n = 66). Each diet provided 36%TE total fat. Dietary targets were broadly met for all intervention groups, reaching 17.6 ± 0.4%TE SFA, 18.5 ± 0.3%TE MUFA, and 10.4 ± 0.3%TE n-6 PUFA in the respective diets, with significant overall diet effects for the changes in SFAs, MUFAs, and n-6 PUFAs between groups (P fat, protein, carbohydrate, and alcohol intake or anthropometric measures between groups. Plasma phospholipid FA composition showed changes from baseline in the proportions of total SFAs, MUFAs, and n-6 PUFAs for each diet group, with the changes in SFAs and MUFAs differing between the groups (P < 0.001). In conclusion, successful implementation of the food-exchange model broadly achieved the dietary target intakes for the exchange of SFAs with MUFAs or n-6 PUFAs with minimal disruption to the overall diet in a free-living population. This trial was registered at clinicaltrials.gov as NCT01478958. © 2014

  • O Enigma do Mal: uma leitura do 'De casu diaboli'de Santo Anselmo (The enigma of evil: a reading of St. Anselm’s De casu diaboli - DOI: 10.5752/P.2175-5841.2013v11n32p1551

    Directory of Open Access Journals (Sweden)

    Manoel Luís Cardoso Vasconcellos

    2013-12-01

    Full Text Available O presente artigo quer investigar o problema do mal na perspectiva de Santo Anselmo, tomando como ponto de partida sua obra De casu diaboli, onde o tema é abordado, a partir da íntima ligação entre mal e pecado. Ao enfocar o mal na perspectiva da queda diabólica, o Doutor Magnífico intenta mostrar a conexão entre vontade, liberdade e justiça. Sua preocupação é investigar o significado moral da má escolha, realizada por uma criatura racional e puramente espiritual, feita boa e para ser boa. O pecado, consoante o autor, não está em almejar a felicidade, pois tal desejo é dádiva divina; o mal praticado pelo anjo está, precisamente, em querer a felicidade fora da justiça, isto é, ao querer ser feliz, ele foi de encontro ao que ele deveria ser: quis ser semelhante a Deus, extrapolando a sua própria natureza, pois ele desejou algo que, enquanto criatura, não caberia a ele almejar. Este é o ponto de partida para analisar o mal e sua mais dramática consequência, o sofrimento. Palavras-chave: Mal. Vontade. Justiça. Anselmo.   Abstract This article aims to investigate the problem of evil in Saint Anselm, especially in his treatise De casu diaboli. He approaches this issue from the close connection between evil and sin. Upon analyzing evil from the diabolic fall perspective, Dr. Magnificent tries to establish a connection between will, freedom and justice. This text investigates the moral meaning of misconduct, carried out by a rational and purely spiritual creature that was created good and meant to be good. Seeking for happiness is not itself a sin, according to the author, for such a wish is a gift from God. The evil done by the angel lies in the act of wanting happiness without justice, that is, on wanting to be happy, the angel contradicted what he was supposed to be: he wanted to be like God, transcending his own nature, as he wished for something that, as a creature, he was not fit to wish for. This is the starting point to

    1. Control and prevention of avian influenza in an evolving scenario.

      Science.gov (United States)

      Capua, Ilaria; Marangon, Stefano

      2007-07-26

      Continuing outbreaks of highly pathogenic avian influenza (HPAI) across Eurasia and in Africa, caused by a type A influenza virus of the H5N1 subtype appear out of control and represent a serious risk for animal and public health worldwide. It is known that biosecurity represents the first line of defence against AI, although in certain circumstances strict hygienic measures appear to be inapplicable for social and economic conditions. The option of using vaccination against AI viruses of the H5 and H7 subtypes, has made its way in recent times--primarily as a tool to maximise the outcome of a series of control measures in countries that are currently infected, but also as a means of reducing the risk of introduction in areas at high risk of infection. In developing countries vaccination programmes in avian species have been recommended recently, however it will require concurrent management of local husbandry practices and industry compliance to eradicate the disease rather than the establishment of an endemic situation. Other key deliverables expected for this control strategy include maintaining a major source of food for rural communities and the preservation of the commercial viability of the local poultry industry. In developed countries vaccination is being used as a means of increasing resistance of susceptible animals to reduce the risk of introduction from the reservoir host or to reduce secondary spread in densely populated poultry areas. The recent joint OIE/FAO summits recommended applying vaccination, using the differentiating infected from vaccinated animals (DIVA) strategy when there is risk of major spread and depopulation is not feasible or desirable. Particularly in developing countries, stamping out of infected animals does not seem to be an appropriate means of reducing the spread of infection, if food supplies are to be guaranteed and economic consequences minimised. Crucial points to the success of a vaccination campaign are the

    2. Diversification of the yellow-shouldered bats, genus Sturnira (Chiroptera, Phyllostomidae), in the New World tropics.

      Science.gov (United States)

      Velazco, Paúl M; Patterson, Bruce D

      2013-09-01

      The Yellow-shouldered bats, Genus Sturnira, are widespread, diverse, and abundant throughout the Neotropical Region, but little is known of their phylogeny and biogeography. We collected 4409 bp of DNA from three mitochondrial (cyt-b, ND2, D-loop) and two nuclear (RAG1, RAG2) sequences from 138 individuals representing all but two recognized species of Sturnira and five other phyllostomid bats used as outgroups. The sequence data were subjected to maximum parsimony, maximum likelihood, and Bayesian inference analyses. Results overwhelmingly support the monophyly of the genus Sturnira but not continued recognition of Corvira as a subgenus; the two species (bidens and nana) allocated to that group constitute separate, basal branches on the phylogeny. A total of 21 monophyletic putatively species-level groups were recovered; pairs were separated by an average 7.09% (SD=1.61) pairwise genetic distance in cyt-b, and three of these groups are apparently unnamed. Several well-supported clades are evident, including a complex of seven species formerly confused with S. lilium, a species that is actually limited to the Brazilian Shield. We used four calibration points to construct a time-tree for Sturnira, using BEAST. Sturnira diverged from other stenodermatines in the mid-Miocene, and by the end of that epoch (5.3 Ma), three basal lineages were present. Most living species belong to one of two clades, A and B, which appeared and diversified shortly afterwards, during the Pliocene. Both parsimony (DIVA) and likelihood (Lagrange) methods for reconstructing ancestral ranges indicate that the radiation of Sturnira is rooted in the Andes; all three basal lineages (in order, bidens, nana, and aratathomasi) have strictly or mainly Andean distributions. Only later did Sturnira colonize the Pacific lowlands (Chocó) and thence Central America. Sturnira species that are endemic to Central America appeared after the final emergence of the Panamanian landbridge ~3 Ma. Despite its

    3. Distribución potencial del híbrido Pleopeltis polylepis (Roemer ex Kunze T. Moore var. polylepis x Polypodium guttatum Maxon (Polypodiaceae Pteridophyta: Áreas probables de simpatría

      Directory of Open Access Journals (Sweden)

      María Ixhel Farfán-Roldán

      2015-08-01

      Full Text Available A través de los sistemas de información geográfica se pueden analizar variables biológicas y ambientales para generar modelos predictivos de distribución geográfica, útiles en la actualidad en estudios de biogeografía, genética, biología de la conservación y evolución de las especies. Farfán-Roldánet al. (2006 describieron un nuevo híbrido entrePleopeltis polylepisvar.polylepisyPolypodium guttatumcolectado en el Parque Nacional El Chico, en el estado de Hidalgo, y de este trabajo surgió el interés de estudiar la distribución de los progenitores de esta planta para obtener áreas de simpatría. Con este motivo se realizaron dos tipos de acercamiento: primero un análisis de la distribución geográfica de los progenitores, apoyados en ejemplares de diversos herbarios que fueron debidamente georeferenciados y se ubicaron en un mapa de la República Mexicana con un grid de medio grado, en segundo término se determinaron las áreas de distribución potencial con un análisis de variables bioclimáticas por medio del programa DIVA-GIS. Fue así que con base en los resultados obtenidos se proponen cinco áreas potenciales de distribución donde puede encontrarse el híbrido: la primera en Nuevo León, Tamaulipas y Coahuila, la segunda en el estado de Guanajuato, Jalisco y Zacatecas; la tercera en los estados de Querétaro, San Luís Potosí y parte de Guanajuato; otra en Hidalgo, Estado de México, Michoacán y el Distrito Federal, y una última comprende un área en los estados de Hidalgo, Puebla y Tlaxcala. El hecho de compartir los mismos hábitats y la proximidad filogenética de estos taxa son factores que pueden influir en la formación de los híbridos.

    4. ITS2 secondary structure improves phylogeny estimation in a radiation of blue butterflies of the subgenus Agrodiaetus (Lepidoptera: Lycaenidae: Polyommatus

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      Wolf Matthias

      2009-12-01

      Full Text Available Abstract Background Current molecular phylogenetic studies of Lepidoptera and most other arthropods are predominantly based on mitochondrial genes and a limited number of nuclear genes. The nuclear genes, however, generally do not provide sufficient information for young radiations. ITS2 , which has proven to be an excellent nuclear marker for similarly aged radiations in other organisms like fungi and plants, is only rarely used for phylogeny estimation in arthropods, although universal primers exist. This is partly due to difficulties in the alignment of ITS2 sequences in more distant taxa. The present study uses ITS2 secondary structure information to elucidate the phylogeny of a species-rich young radiation of arthropods, the butterfly subgenus Agrodiaetus. One aim is to evaluate the efficiency of ITS2 to resolve the phylogeny of the subgenus in comparison with COI , the most important mitochondrial marker in arthropods. Furthermore, we assess the use of compensatory base changes in ITS2 for the delimitation of species and discuss the prospects of ITS2 as a nuclear marker for barcoding studies. Results In the butterfly family Lycaenidae, ITS2 secondary structure enabled us to successfully align sequences of different subtribes in Polyommatini and produce a Profile Neighbour Joining tree of this tribe, the resolution of which is comparable to phylogenetic trees obtained with COI+COII . The subgenus Agrodiaetus comprises 6 major clades which are in agreement with COI analyses. A dispersal-vicariance analysis (DIVA traced the origin of most Agrodiaetus clades to separate biogeographical areas in the region encompassing Eastern Anatolia, Transcaucasia and Iran. Conclusions With the inclusion of secondary structure information, ITS2 appears to be a suitable nuclear marker to infer the phylogeny of young radiations, as well as more distantly related genera within a diverse arthropod family. Its phylogenetic signal is comparable to the

    5. A first global-scale hindcast of extreme sea levels induced by extra-tropical storms

      Science.gov (United States)

      Muis, Sanne; Verlaan, Martin; Winsemius, Hessel; Ward, Philip

      2015-04-01

      Flood risk in coastal areas has been increasing in past years. This can be partly attributed to climate change and rising sea levels that increase the likelihood of coastal flood hazards, but also to increasing flood exposure because the global population and capital is increasingly concentrated in coastal zones. Without action, the increasing trends in flood hazard and exposure will be associated with catastrophic flood losses in the future. The adequate allocation of global investments and prioritization of adaptation actions requires an accurate understanding of the current and future coastal flood risk on a global-scale. Despite this, global data on extreme sea levels are scarce. A few studies have assessed coastal flood risk at the global-scale. To date, these have been either based on extreme water levels in the DIVA database or on observations from tide gauges. Both datasets have limitations when assessing flood risk, including low-probability events, on a fully global scale. Hence, there is a need for an improved estimation of extreme sea level on a global-scale. Therefore, we are developing the first global hindcast of coastal water levels which covers the period 1979-2013. To do this, we apply a global hydrodynamic model which is based on the Delft3D Flexible Mesh software from Deltares. By forcing the model with the tidal potential and meteorological fields derived from the ERA-Interim global reanalysis, we are able to simulate the water levels resulting from tides and surges. Subsequently, we apply extreme value statistics to estimate exceedance probabilities. Similar hydrodynamic modelling efforts have been carried out at the regional scale, but as the modelling of surges in shallow coastal areas requires a high-resolution model grid, generally this approach is computationally too costly on a global-scale. However, the recent application of unstructured grids (or flexible mesh) in hydrodynamic models, allowing local refinement of the grid, has enabled

    6. SURTO DE SARNA CORIÓPTICA EM CAPRINOS NA CIDADE DE GRAVATÁ, REGIÃO AGRESTE DO ESTADO DE PERNAMBUCO OUTBREAK OF CHORIOPTIC MANGE IN GOATS IN THE CITY OF GRAVATÁ IN PERNAMBUCO´S AGRESTE REGION

      Directory of Open Access Journals (Sweden)

      Maria Isabel de Souza

      2009-12-01

      Full Text Available <div>A sarna corióptica é de rara ocorrência em caprinos, no entanto trata-se de uma enfermidade cutânea importante, resultando em perdas econômicas decorrentes do retardo no desenvolvimento dos animais, da perda de peso e do prejuízo à indústria de couro. Desse modo, o objetivo do presente artigo é relatar a ocorrência de um surto de sarna corióptica em um rebanho caprino criado no município de Gravatá (Lat. 08° 12’ 04’’ S e Long. 35° 33’ 53’’ O, região Agreste do estado de Pernambuco. Os animais apresentavam extensas áreas de alopecia, eritema, crostas e espessamento da pele na região das axilas, peito, ventre e as faces caudal e craniomedial dos membros posteriores, além de intenso prurido. Foi realizado raspado cutâneo e identificado o ácaro Chorioptes bovis spp.


    PALAVRAS-CHAVES: Caprinos, Chorioptes spp, sarna.

    Chorioptic mange is of rare occurrence in goats, although it is an important skin disease resulting in economic loss due to the delay in the development of the animals, weight loss and economic losses to the leather industry. Thus, the aim of the present article was to report the occurrence of an outbreak of chorioptic mange in a goat flock reared in the city of Gravatá, (Lat. 08° 12’ 04’’ S and Long. 35° 33’ 53’’ W in Pernambuco´s Agreste region. The animals showed wide alopecia areas, erythema, crusts and skin thickness in the following areas: armpits, sternum, ventral abdomen, caudal and cranium-medial facets of the hind legs and severe scratching. Chorioptes bovis spp was identified in skin scrapings.

    KEY WORDS: Chorioptes spp, goats, mange.

  • Gradient estimate for solutions of a class of nonlinear elliptic equations below the duality exponent

    Science.gov (United States)

    Cirmi, G. Rita; D'Asero, Salvatore; Leonardi, Salvatore

    2017-07-01

    We study the regularity of a solution of the Dirichlet problem associated to the singular equation -div(a (x )D u )+M |/D u | uθ =f (x )inΩ (1) where Ω is an open bounded subset of ℝN (N ≥ 3) with smooth boundary, a(x) is a L∞ -matrix satisfying the standard ellipticity condition, θ ∈]0, 1[, M is a positive constant and f is sufficiently regular i.e. it belongs to a suitable Morrey space Lq,λ (Ω), with q ≥ 1, to be specified later on. We will be concerned with the regularity of the gradient of a solution in Morrey spaces in correspondence with the regularity properties of the right-hand side of the equation (1). There is a huge literature about the problems with quadratic term in the gradient also for high-order equations whose coefficients satisfy a strengthened ellipticity condition (see [5]). The problem (1) has been studied in the paper [2] by D. Arcoya, J. Carmona, T. Leonori, P. J. Martínez-Aparicio, L. Orsina, F. Petitta and in the paper [3] by L. Boccardo where the source term f belonged to Lq(Ω) with q ≥ 1. In [4] we have extended to the gradient of a solution the Morrey property of the right-hand side f and, in some cases, we have improved some results contained in [2, 3] without increasing the summability of f. Here we will be concerned with an intermediate case, in the sense that the right hand side is not merely a measure and it doesn't belong to the right dual space (see [10, 9]). In obtaining the necessary local estimates we faced the problem of performing the Campanato's decomposition of the solution due to the presence of the degenerate lower order term. We had thus to adopt an alternative method already used in [4]. This is part of a new set of estimates, including potential estimates, as for instance in [6, 7, 8], that would be very interesting to extend in the present setting too.

  • 珍稀濒危植物伯乐树的潜在适生区预测%Prediction of potential distributions of Bretschneidera sinensis,an rare and endangered plant species in China

    Institute of Scientific and Technical Information of China (English)

    龚维; 夏青; 陈红锋; 俞新华; 伍菲

    2015-01-01

    [目的]揭示伯乐树Bretschneidera sinensis地理分布格局,预测其潜在适生区,探讨环境和气候因子对其地理分布格局的影响.[方法]基于1950-2000年期间及21世纪70年代的19个气候因子数据以及中国13个省区、110个有记录的地理分布点,利用最大熵算法模型(Maxent),结合地理软件DIVA-GIS和ARCGIS,预测伯乐树的潜在适生区.结合野外调查和标本数据,以海拔图层和植被覆盖图层为底图绘制该种地理分布.[结果和结论]伯乐树潜在适生区位于华南地区,最适分布区位于南岭地区以及罗霄山和幕阜山.该种常见于海拔500~2 000 m的山地常绿阔叶林、常绿针叶林和落叶阔叶林,适宜气候条件为最暖季度平均雨量500 ~2 750 mm,昼夜温差7.8~9.8 ℃.随着全球气候变化,该种潜在适生区北移,最适分布区面积减少.基于此,还分析了主导环境和气候因子对该种地理分布格局的影响,并对其保护策略提出了建议.

  • Characteristics and determinants of knowledge transfer policies at universities and public institutions in medical research--protocol for a systematic review of the qualitative research literature.

    Science.gov (United States)

    Jahn, Rosa; Müller, Olaf; Bozorgmehr, Kayvan

    2015-08-19

    Universities, public institutions, and the transfer of knowledge to the private sector play a major role in the development of medical technologies. The decisions of universities and public institutions regarding the transfer of knowledge impact the accessibility of the final product, making it easier or more difficult for consumers to access these products. In the case of medical research, these products are pharmaceuticals, diagnostics, or medical procedures. The ethical dimension of access to these potentially lifesaving products is apparent and distinguishes the transfer of medical knowledge from the transfer of knowledge in other areas. While the general field of technology transfer from academic and public to private actors is attracting an increasing amount of scholarly attention, the specifications of knowledge transfer in the medical field are not as well explored. This review seeks to provide a systematic overview and analysis of the qualitative literature on the characteristics and determinants of knowledge transfer in medical research and development. The review systematically searches the literature for qualitative studies that focus on knowledge transfer characteristics and determinants at medical academic and public research institutions. It aims at identifying and analyzing the literature on the content and context of knowledge transfer policies, decision-making processes, and actors at academic and public institutions. The search strategy includes the databases PubMed, Web of Science, ProQuest, and DiVa. These databases will be searched based on pre-specified search terms. The studies selected for inclusion in the review will be critically assessed for their quality utilizing the Qualitative Research Checklist developed by the Clinical Appraisal Skills Programme. Data extraction and synthesis will be based on the meta-ethnographic approach. This review seeks to further the understanding of the kinds of transfer pathways that exist in medical

  • Recent development of infrasound monitoring network in Romania

    Science.gov (United States)

    Ghica, Daniela; Popa, Mihaela; Ionescu, Constantin

    2017-04-01

    The second half of 2016 was marked at National Institute for Earth Physics (NIEP) by a significant development of infrasound monitoring infrastructure in Romania. In addition to IPLOR, the 6-element acoustic array installed at Plostina, in the central part of Romania, since 2009, two other four-element arrays were deployed. The first one, BURARI infrasound research array, was deployed in late July 2016, under a joint effort of AFTAC, USA and NIEP, in the northern part of Romania, in Bucovina region. The sites, placed in vicinity of the central elements of BURAR seismic array (over 1.2 km aperture), are equipped with Chaparral Physics Model 21 microbarometers and Reftek RT 130 data loggers. The data, used mainly for research purposes within the scientific collaboration project between NIEP and AFTAC, are available to scientific community. The second one is a PTS portable infrasound array (I67RO) deployed for one year, starting with the end of September 2016, within a collaboration project between NIEP and PTS of the Preparatory Commission for CTBTO. This array is located in the western part of Romania, at Marisel, Cluj County, covering a 0.9 km aperture and being equipped with CEA/DAM MB2005 microbarometers and Reftek RT 130 data loggers. This joint experiment aims to contribute both to advanced understanding of infrasound sources in Central-Europe and to ARISE design study project, as an expansion of the spatial coverage of the European infrasound network. The data recorded by the three infrasound arrays deployed in Romania, during a same time interval (October - December 2016) were processed into detection arrival bulletins applying CEA/DASE PMCC algorithm embedded in DTK-GPMCC (extended CTBTO NDC-in-a-box) and WinPMCC software applications. The results were plotted and analyzed using DTK-DIVA software (extended CTBTO NDC-in-a-box), in order to assess detectability of each station, as well as the capacity of fusing detections into support of infrasound monitoring

  • Functional diversity of microbial communities in pristine aquifers inferred by PLFA- and sequencing-based approaches

    Science.gov (United States)

    Schwab, Valérie F.; Herrmann, Martina; Roth, Vanessa-Nina; Gleixner, Gerd; Lehmann, Robert; Pohnert, Georg; Trumbore, Susan; Küsel, Kirsten; Totsche, Kai U.

    2017-05-01

    Microorganisms in groundwater play an important role in aquifer biogeochemical cycles and water quality. However, the mechanisms linking the functional diversity of microbial populations and the groundwater physico-chemistry are still not well understood due to the complexity of interactions between surface and subsurface. Within the framework of Hainich (north-western Thuringia, central Germany) Critical Zone Exploratory of the Collaborative Research Centre AquaDiva, we used the relative abundances of phospholipid-derived fatty acids (PLFAs) to link specific biochemical markers within the microbial communities to the spatio-temporal changes of the groundwater physico-chemistry. The functional diversities of the microbial communities were mainly correlated with groundwater chemistry, including dissolved O2, Fet and NH4+ concentrations. Abundances of PLFAs derived from eukaryotes and potential nitrite-oxidizing bacteria (11Me16:0 as biomarker for Nitrospira moscoviensis) were high at sites with elevated O2 concentration where groundwater recharge supplies bioavailable substrates. In anoxic groundwaters more rich in Fet, PLFAs abundant in sulfate-reducing bacteria (SRB), iron-reducing bacteria and fungi increased with Fet and HCO3- concentrations, suggesting the occurrence of active iron reduction and the possible role of fungi in meditating iron solubilization and transport in those aquifer domains. In more NH4+-rich anoxic groundwaters, anammox bacteria and SRB-derived PLFAs increased with NH4+ concentration, further evidencing the dependence of the anammox process on ammonium concentration and potential links between SRB and anammox bacteria. Additional support of the PLFA-based bacterial communities was found in DNA- and RNA-based Illumina MiSeq amplicon sequencing of bacterial 16S rRNA genes, which showed high predominance of nitrite-oxidizing bacteria Nitrospira, e.g. Nitrospira moscoviensis, in oxic aquifer zones and of anammox bacteria in more NH4+-rich

  • Human leucocytic antigen-DR negative acute myeloid leukemia: A diagnostic dilemma for hematopathologist

    Directory of Open Access Journals (Sweden)

    Ashish Gupta

    2014-01-01

    Full Text Available Background: Acute myeloid leukemia (AML blast variably express Human leucocytic antigen (HLA.We retrospectively analyzed immunophenotypic and clinical profile of 12 cases of HLA -DR negative AML and correlated with their morphological, cytogenetics and Molecular findings.There is a paucity of literature mentioning morphological, immunophenotypic and cytogenetics characteristics of HLA DR negative AML. Aim: This study was designed to study the morphological, flow cytometric, and cytogenetics characteristics of HLA DR negative AML/non acute Promyelocytic Leukaemia (APML cases. Materials and Methods: Seventeen such cases were diagnosed over a period of 1 year and 8 months. Peripheral blood and bone marrow aspiration smears were stained by Wright giemsa and examined by three hematopathologist independently. Immunophenotyping was done using multicolour flow cytometry on BD FACS CANTO II using FACS DIVA software.Conventional Karyotyping was done using Wright giemsa staining (using IKAROS software and florescent in situ hybridization (FISH was done using dual color dual fusion probe from Vysis promyelocytic leukemia-retinoic acid receptor alpha (PML-RARA fusion gene probe. Molecular analysis using reverse transcriptase-polymerase chain reaction (RT-PCR was done using Thermal Cycler of Applied Biosystem and Gel-Doc by Biorad. Results : Of the 12 cases studied ten were classified as French-American-British (FAB AML-M1. Two case as FAB AML-M2. Morphologically the cells resemble abnormal promyelocytes with bilobation, convoluted and folded nucleus, inconspicuous nucleoli and open chromatin (n = 11 and with blastic morphology, open chromatin, and inconspicuous nucleoli (n = 1.Karyotyping analysis shows normal karyotype (n = 10, del 9q-(n = 1, and t (5:9 (n = 1 respectively.FISH done using dual color dual fusion probe (n = 12 do not show PML-RARA fusion signal.RT-PCR (n = 12 revealed a negative result for PML - RARA fusion transcripts. Conclusion: HLA

  • Modeling of Geographic Distribution of Malus doumeri%台湾林檎(Malus doumeri(Bois.) Chev.)地理分布模拟

    Institute of Scientific and Technical Information of China (English)

    王雷宏; 杨俊仙; 郑玉红; 汤庚国

    2012-01-01

    Specimens of Malus doumeri (Bois. ) Chev. from some herbaria, including the herbarium of Institute of Botany, Chinese Academy of Sciences (PE) , were re-identified in light of the latest taxonomic outcome of the genus Malus Mill. A total of 243 valuable distribution records were selected and digitized into the geographic coordinate data. Based on these data, the geographical distribution was mapped using DIVA-GIS. This distribution presents a pattern extending mainly from the southwest to southern and east in China, and disconnecting between Chinese Continent and Taiwan Island. It is almost consistent with the distribution of specimen records modeling with current bioclimatic data by BIOCLIM, and some potential distribution areas were found. Nineteen climate variables of the distribution points that fell in the envelope of variables of the Asia climate (spatial resolution 30 arc s) were analyzed by principal component analysis. Precipitation of wettest quarter, annual range of temperature, and precipitation of driest quarter were found to be dominant factors affecting the geographic distribution of M. doumery. Niches of dominant factors were interval only, and other niches overlapped among the varieties. The present and future potential distribution maps were predicted by BIOCLIM with current and future bioclimatic data (spatial resolution 30 arc s). Results showed that the overall distribution profile almost did not change, and it changed only in the scope and size of suitable area for each level within the entire distribution area. Based on the characteristics of ecological requirements and distribution trends of M. doumeri, the original region of M. doumeri is speculated in the borders between Yunnan, Cuangxi, Laos and Vietnam.%依据苹果属最新的系统分类学成果,重新鉴定了中国科学院植物研究所等标本馆的标本,选取了243个有价值的分布记录转换成地理坐标数据,用DIVA-GIS绘制了采集标本的

  • Calidad postcosecha de tomates almacenados en atmósferas controladas Postharvest quality of tomato fruits stored under controlled atmospheres

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    Perla A. Gómez

    2002-03-01

    Full Text Available Las atmósferas controladas alargan la vida en estante del tomate, permitiendo su cosecha en estados de madurez más avanzados y a su vez afectando su calidad. El objetivo de este experimento fue estudiar los efectos que atmósferas controladas con bajas concentraciones de oxígeno y libres de dióxido de carbono y etileno, producen sobre el color, la firmeza y los principales componentes del sabor de frutos de tomate cultivar Diva. Durante abril de 1998, frutos cosechados con grado de color 3 (USDA en INTA Balcarce (Argentina fueron almacenados en frascos herméticos de 3 L a 12°C durante 36 días. El diseño fue completamente aleatorizado con tres repeticiones. Cada frasco poseía cuatro frutos, los que constituyeron la unidad experimental. Los tratamientos fueron cuatro, consistiendo en un flujo continuo de: a 1%, b 3%, c 5% y d 21% de O2 (testigo completando con N2 hasta 100%. El color, medido con un colorímetro, evolucionó más lentamente y la firmeza se retuvo por más tiempo a medida que se redujo la concentración de oxígeno. Con 5% de oxígeno el color final no difirió del testigo, con 3% sólo fue igual al exponerlos luego a 20°C, no alcanzándose una coloración normal con 1%. Por otra parte, y en todos los tratamientos por igual, los sólidos solubles y la acidez fueron menores y el pH fue mayor que al inicio. El color y la firmeza preferidos por los consumidores (Índice de color »12 y firmeza » 65 KPa fueron alcanzados a los 7 días de almacenamiento con 21% de O2, a los 12 con 5%, a los 14 con 3% y a los 21 con 1%, correspondiéndose con valores decrecientes de la relación sólidos solubles/acidez titulable. Esto indicaría que frutos externamente semejantes poseerían propiedades organolépticas diferentes. Los efectos observados estarían asociados no sólo a la menor tensión de oxígeno, sino también a la ausencia de etileno.Controlled atmosphere extends tomato shelf life, allowing them to be harvested them at later

  • Acumulação de matéria seca do tomateiro cultivado em substrato com diferentes doses de fertilizantes Dry matter accumulation of tomato plants grown in substrate under different fertilizer doses

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    Roberto André Grave

    2001-10-01

    Full Text Available Plantas de tomateiro foram cultivadas em substrato no interior de um ambiente protegido localizado no Departamento de Fitotecnia da UFSM, em Santa Maria, RS, com o objetivo de determinar o efeito de cinco doses de fertilizantes sobre a acumulação da matéria seca da parte vegetativa e dos frutos. Foram efetuados três experimentos, no outono-inverno de 1997, na primavera-verão de 1997 e no outono-inverno de 1998, empregando os híbridos Diva, Monte Carlo e Saladette, respectivamente. Foi utilizada como referência uma dose semanal com as seguintes quantidades de fertilizantes, em g por planta: 4 de KNO3; 6,3 de Ca(NO32; 1,5 de superfosfato simples; 3 de MgSO4, complementada por 0,13ml de ferro quelatizado (5% de Fe e 0,7mL de uma solução contendo os demais micronutrientes. No experimento 1, a dose de T1 foi igual àquela de referência e as doses de T2 e T3 foram obtidas multiplicando-se a dose de referência por 1,15 e 1,3, respectivamente. Nos experimentos 2 e 3, as doses de T1, T2 e T3 corresponderam à dose de referência multiplicada por 0,6; 1,15 e 1,7, respectivamente. Para cada um dos dois híbridos e nos três experimentos, as plantas foram localizadas em três blocos, com três fileiras de 35 plantas em cada bloco. Cada tratamento foi aplicado uma vez por semana a todas as plantas da mesma fileira, através da fertirrigação. Foi determinada a matéria seca acumulada na parte vegetativa da planta e nos frutos no início do período de maturação dos mesmos, em cada experimento. Não foram observadas diferenças significativas na acumulação de matéria seca na parte vegetativa da planta e nos frutos entre as três doses e nos três experimentos.Tomato plants were grown in substrate in a greenhouse at Departamento de Fitotecnia, Universidade Federal de Santa Maria, RS, Brazil, to determine the effect of five fertilizer levels on dry matter accumulation of vegetative plant parts and fruits. Three experiments were done, in autumn

  • 音域图测试在声带息肉中的应用%The applications of the voice range profile in the polyp of vocal cord

    Institute of Scientific and Technical Information of China (English)

    方红雁; 李晓晓; 李劲松; 廖修富; 李忠万

    2015-01-01

    Objective To analyze the pathological voice ,normal voice range chart crowd voice characteristics through tests and analyze the differences between the two groups of test‐related parameters chart range between indicators ,so as to explore the value of the test range map in the polyp of vocal cord .Methods One hundred and twenty patients with vocal polyps according to in‐clusion and exclusion criteria were selected .Sixty cases of normal voice were randomly selected .Germany XION DIVAS system dia‐gram test mode range were recorded for both groups ,with the strongest voice of the bass were measured at different frequencies , first obtain low VRP intensity curve ,then get high‐intensity curve VRP .The maximum frequency of the vocal cord polyp group and normal control group ,the minimum frequency ,maximum intensity ,most quietly strong ,maximum phonation time ,fundamental fre‐quency perturbation and dysphonia index of the data were compared statistically .Results Compared with the control one ,the mean maximum frequency of vocal cord polyp group were lower (P= 0 .029 6) ,the average minimum frequency were significantly higher ( P= 0 .000 3) ,frequency range reduced(P= 0 .022 9) ;vocal cord polyp average loudest group was stronger(P= 0 .003 9) ,the whis‐pered strength values were significantly higher (P = 0 .000 0) ,significantly limited the average sound intensity range than normal voice group group (P= 0 .006 9) .Classification index based on objective voice disorders ,in terms of frequency ,mild voice disorders at the maximum frequency .There was no significant difference in the minimum frequency ,frequency range with a normal voice group ;and in severe voice disorders average maximum frequency lower than the normal voice group ,the smallest than normal voice frequency was significantly higher frequency range than the normal group was significantly limited .In terms of strength ,mild im‐pairment in the loudest voice is strong ,most quietly strong .There

  • La Asistencia Social vista por pobres y nuevos pobres en Buenos Aires durante la Convertibilidad A assistência social a partir da perspectiva dos pobres e novos pobres na cidade de buenos aires durante o período da “convertibilidad”

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    Patricia Aguirre

    2009-12-01

    Full Text Available Abordaremos a assistência alimentícia concedida entre os anos de 1991 e 2001 (durante o programa econômico conhecido como Convertibilidad e seus efeitos em dois grupos familiares: os pobres e aqueles considerados “novos pobres” na área metropolitana da cidade de Buenos Aires, Argentina. Será analisado o Gasto Público Social (expressivamente reduzido e o subsídio (negativo dado às famílias na forma de assistência alimentícia, comparando com os impostos arrecadados no consumo e os conceitos que regem a assistência social: equidade, eficiência, focalização e participação. Os resultados dos “Estudos de Caso” realizados com 144 famílias serão expostos, sublinhando as diferentes percepções a com relação ao Estado (eles e nós; b da assistência alimentícia (entendida entre os pobres como uma dádiva e entre os novos pobres como um direito; e c os efeitos da focalização (entendida como exclusão e participação (troca do trabalho por alimentosAbordaremos la asistencia alimentaria entre 1991 y 2001 (durante el programa económico llamado: Convertibilidad y sus efectos en 2 grupos de familias: pobres y “nuevos pobres” del Área Metropolitana Bonaerense.Analizaremos el Gasto Público Social (marcadamente regresivo, el subsidio (negativo que reciben las familias en forma de asistencia alimentaria comparándolo con lo que aportan como impuestos al consumo y los conceptos rectores de la asistencia: equidad, eficiencia, focalización y participación. Expondremos los resultados del “estudio de casos” que realizamos con 144 familias advirtiendo las diferencias en las percepciones a del estado (ellos/nosotros, b de la asistencia alimentaria (entendida en pobres como dádiva y en nuevos pobres como derecho y, c los efectos de la focalización (vista como exclusión y la participación (trueque de trabajo por alimentosThis article deals with food assistance provided in the metropolitan area of Buenos Aires between 1991

  • EFEITO DA SUPLEMENTAÇÃO PROTÉICA SOBRE OS PARÂMETROS CLÍNICOS E PARASITOLÓGICOS DE OVINOS MANTIDOS EM PASTAGEM DE TIFTON 85 EFFECT OF PROTEIN SUPPLEMENTATION ON THE CLINICAL AND PARASITOLOGICAL PARAMETERS OF LAMBS UNDER PASTURE OF TIFTON 85

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    Daniel Maia Nogueira

    2009-12-01

    Full Text Available <div>A suplementação proteica pode ser uma importante ferramenta para os sistemas de produção de ovinos em pastagens tropicais. Objetivou-se com este trabalho avaliar os parâmetros clínicos e parasitológicos de ovinos mantidos em pastagem de Tifton 85 (Cynodon dactylon irrigada, recebendo suplementos com diferentes fontes proteicas. Foram utilizados 28 ovinos castrados e mestiços, distribuídos homogeneamente em quatro tratamentos. Além do controle não suplementado, os tratamentos avaliados foram: farelo de soja, ureia e torta de algodão. Realizou-se a vermifugação dos animais de acordo com o método Famacha©. Não houve diferença significativa (P>0,05 entre os tratamentos para o consumo de matéria seca total, ganho médio diário e ganho de peso total. Foi observado maior consumo de forragem (P<0,05 para os animais mantidos exclusivamente em pastagem. Estes animais também apresentaram maior contagem de ovos por grama de fezes (OPG (P<0,05 em comparação aos suplementados com ureia ou com torta de algodão. Não houve diferença significativa (P>0,05 para os diferentes tons de coloração da conjuntiva nem para o número de animais vermifugados. Observou-se uma prevalência de 72,0% a 83,0% de larvas de Trichostrongylus sp. As diferentes suplementações proteicas não influenciaram as características clínicas nem produtivas dos animais.


  • PALAVRAS-CHAVES: Cynodon dactylon, endoparasitas, Famacha©, farelo de soja, torta de algodão, ureia.
    The protein supplementation may be an important tool for sheep production systems in tropical grazing. This work aimed to evaluate parasitological and clinical aspects of lambs under irrigated pasture of Tifton 85 (Cynodon dactylon and receiving supplementation from different protein sources. Twenty-eight, castrated and crossbreed lambs, were used as animal testers and allocated into four treatments. Besides the control with exclusively use of pasture

  • FECES CHARACTERISTICS AND FAECAL SAND EXCRETION IN EQUINE KEEP AT PASTURE IN CACHOEIRO ITAPEMIRIM CITY, ESPÍRITO SANTO, BRAZIL CARACTERÍSTICA DAS FEZES E EXCREÇÃO FECAL DE AREIA EM EQUINOS MANTIDOS A PASTO NO MUNICÍPIO DE CACHOEIRO DO ITAPEMIRIM, ESPÍRITO SANTO, BRASIL

    Directory of Open Access Journals (Sweden)

    Sílvia Araújo França

    2009-12-01

    Full Text Available

    The characteristic of feces and fecal excretion of sand were evaluated in 112 mares in the Cachoeiro do Itapemirim city, state of Espírito Santo, Brazil. From each animal were collected 200 grams of feces by transrectal palpation, and after collection were evaluated for consistency, hydration, color, smell, size of fiber, grains and presence of foreign body. Simultaneously was performed the test of sedimentation of sand to determine the fecal excretion of sand. In 100% of animals feces were firm with the unique odor. As for color, 90% (101/112 of the animals showed feces of moss green color, while 10% (11/112 showed dark olive green stool. For consistency, 23.21% (26/112 had stool slightly pasty and 76.79% (86/112 had stool firm. Regarding the degree of hydration, 23.21% (26/112 had stool with slight increase in water content, while 76.79% (86/112 had stool with normal hydration. The test of sedimentation of the sand revealed that 100% (112/112 of animals excreted sand at the time of the study. Although the prevalence of fecal excretion of sand has been high, the presence of sand in the gastrointestinal tract of animals evaluated is not causing gastroenteric changes.

    KEY WORDS: Equine, feces, sand.

    <div>A característica das fezes e a excreção fecal de areia foram avaliadas em 112 éguas criadas a campo no município de Cachoeiro do Itapemirim, estado do Espírito Santo, em um delineamento inteiramente casualizado. Colheram-se de cada animal 200 gramas de fezes por palpação transretal, sendo estas avaliadas quanto à consistência, ao grau de hidratação, à coloração, ao odor, ao tamanho da fibra, à presença de grãos e de corpo estranho. Simultaneamente, foi realizado o teste de sedimentação de areia para determinar a excreção fecal de areia. Em 100% dos animais, as síbalas eram firmes com odor sui generis. Quanto à coloração, 90% (101/112 dos animais apresentaram fezes

  • BLUETONGUE VIRUS ANTIBODIES DETECTIONS IN SHEEP FROM ARAÇATUBA REGION –SAO PAULO, BRAZIL DETECÇÃO DE ANTICORPOS CONTRA O VÍRUS DA LÍNGUA AZUL EM OVINOS NA REGIÃO DE ARAÇATUBA – SÃO PAULO, BRASIL

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    Adriana Hellmeister de Campos Nogueira

    2009-12-01

    Full Text Available

    Bluetongue (BT is an infectious, insect-born viral disease of ruminants. The causative agent of BT is bluetongue virus (BTV that belongs to the family Reoviridae genus Orbivirus. Insect vectors in the genus Culicoides transmit this virus. BT affects domestic and wild ruminants, however small ruminants are considered the most affected specie. The aim of the study was to detect antibodies against BTV in commercial sheep farms, of the Northeastern region of Sao Paulo State, Brazil. A total of 1002 sera samples collected from adult sheep (above 1 year-old, comprising a total of 31 farms, were screened for the presence of BTV antibodies, by agar gel immunodiffusion test (AGID and ELISA-CFS (Enzyme Linked Immunosorbent Assay – competitive solid phase, both produced by Pan American Center of FMDV. From a total of 1002 samples, 651 (65% were positive by AGID and 742 (74.1%, were positive by ELISA-CFS. These results suggest that the BTV is widespread among farms, probably causing subclinical infections.

    KEY WORDS: AGID, bluetongue virus, ELISA-CFS, seroepidemiological survey.

    <div>A língua azul é uma doença viral, cujo agente etiológico pertence à família Reoviridae, gênero Orbivirus, transmitida por um vetor (artrópode hematófago, do gênero Culicoides. Os animais acometidos são ruminantes domésticos e selvagens, porém os pequenos ruminantes são os mais afetados. O estudo teve como objetivo detectar a presença de anticorpos para língua azul em ovinos da região de Araçatuba, por possuir um rebanho expressivo e condições climáticas favoráveis à multiplicação de insetos. Foram analisadas 1.002 amostras de soros ovinos, provenientes de 31 cabanhas, pelas provas de imunodifusão dupla em gel de ágar (AGID e ELISA (Enzyme Linked immunosorbent Assay de competição da fase sólida (ELISA CFS, provenientes do Centro Panamericano de Febre Aftosa. Desses soros, 651 (65% foram

    1. THE POLEN PAULLINIEAE (SAPINDACEAE EL POLEN DE PAULLINIEAE (SAPINDACEAE

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      María Silvia Ferrucci

      2010-11-01

      Full Text Available <div>A detailed study on the morphology of pollen on thirty one species of the tribe Paulliniieae, comprising seven genera, has been carried out by LM and SEM.

    The pollen grains of the following taxa were examined: subtribe Paulliniinae (Cardiospermum L., 4 spp., Houssayanthus Hunz., 2 spp., Lophostigma Radlk., 1 sp., Paullinia L., 3 spp., Serjania Miller, 16 spp., and Urvillea Kunth, 4 spp.; subtribe Tlúnouúnae (Thinouia Triana & Planchon, 1 sp.. All of them grow in Argentina, except L. plumosum Radlk. and H. monogynus (Schdl. Ferrucci, collected in Bolivia and Paraguay respectively.
    Four pollen types have been recognized: type A - isopolar, tricolporate, subspheroidal to oblate-spheroidal grains in Thinouia; type B - isopolar or subisopolar, trisyncolporate, heterocolpate, dicolpodiorate, monocolpate, prolate or prolate-spheroidal grains in Lophostigma; type C - heteropolar, hemitrisyncolporate, peroblate or oblate grains are shared by Serjania, Houssayanthus, Cardiospermum and Urvillea, (in these genera tbree groups are recognized based on details of structure and sculpture and type D - isopolar or subisopolar triporate, oblate or peroblate grains in Paullinia.
    The probable evolutionary trends of pollen morphology cbaracters are discussed. Based on shape and aperture types of the grains, a tentative phylogenetic line of the tribe is presented
    Un estudio detallado sobre la morfología del polen en treinta y una especie de la tribu Paullinieae, que comprende siete géneros, se ha llevado a cabo por LM y SEM.
    Fueron examinados los granos de polen de las siguientes especies: subtribu Paulliniinae (Cardiospermum L., 4 spp, Houssayanthus Hunz, 2 spp, Lophostigma Radlk, 1 sp, Paullinia L., 3 spp, Serjania Miller, 16 spp., y Urvillea Kunth, 4 spp;. subtribu Thinouiinae (Thinouia Triana & Planchon, 1 sp. Todas ellas crecen en la Argentina, salvo Radlk L.plumosum y H.monogynus (Schdl. Ferrucci

  • Produção de Madeira de Progênies de Segunda Geração de Grevílea na Região de Avaré, SP Second Generation Half-sib Progenies Wood Production of Grevillea in the Region of Avaré - SP

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    Emerson Gonçalves Martins

    2011-03-01

    Full Text Available <div>A grevílea (Grevillea robusta A. Cunn é uma espécie utilizada para o sombreamento de cafezais e de outras culturas agrícolas, áreas de pastagens e apicultura, paisagismo e como cortina quebra- ventos. Sua madeira também é utilizada para desdobro, em serrarias. Um teste com 28 progênies de meios-irmãos de segunda geração foi instalado no Município de Avaré, São Paulo. O objetivo principal foi avaliar e selecionar o material genético mais produtivo em volume de madeira. Dados de altura e diâmetro à altura do peito (DAP foram coletados na área experimental, na idade de três anos. Baseando-se nos dados estimados de volume cilíndrico com casca, as seguintes conclusões foram obtidas: em Avaré, SP, a progênie de segunda geração mais produtiva e com maior taxa de sobrevivência pertencente à procedência australiana de Boyd River (NSW. A progênie de segunda geração mais produtiva, em termos de volume cilíndrico de madeira com casca, foi 138 % superior à media geral do teste. Considerando o valor médio de volume de madeira para apenas as doze progênies de segunda geração mais produtivas, foi possível observar um aumento de 32 % em relação à média geral do experimento. 

  • The Grevillea robusta A. Cunn is utilized for coffee plantation shading, crops growing and pasture shading, apiculture, ornamentation and wind breaks. It is also used to produce sawn wood. One test of 28 second generation half-sib progenies was planted in Forestry Institute of São Paulo, with 20°03’S latitude and 48°54’W logitude geographic coordenates and 630m of altitude. The main objectives of this paper were to select genetic material capable to produce more volume of wood. The total heigth and diameter at breast height (DBH were measured on the three yars old second generation half-sib progenies test. The volume was calculated and the results led to the following conclusion: The best

  • Políticas públicas no meio rural: visibilidade e participação social como perspectivas de cidadania solidária e saúde Public policies in rural areas: visibility and social participation as perspectives of supportive citizenship and health

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    Eliziane Nicolodi Francescato Ruiz

    2012-01-01

    Full Text Available O artigo visa estimular o debate sobre o papel que o Estado vem assumindo na construção de uma cidadania mais solidária e na promoção da saúde. Propõe pensar essas relações por meio da Teoria da Dádiva e de categorias analíticas como participação e visibilidade. Parte-se do pressuposto de que, ao circularem bens materiais e imateriais nas políticas públicas, dá-se visibilidade e reconhecimento às necessidades dos usuários, possibilitando abertura para participação e alargamento das parcerias e solidariedades entre Estado-sociedade e sociedade-sociedade. Isso, por sua vez, contribuiria para a concretização de direitos, a potencialização de programas e a materialização da saúde, pensada de uma forma mais ampla. As reflexões aqui apresentadas partem de estudos realizados com abordagem qualitativa, tendo como lócus uma comunidade rural do Rio Grande do Sul. Os dados foram produzidos por meio de entrevistas, observações participantes e grupos focais; na apreciação das informações adotou-se a análise temática. Foi possível apreender que, por um lado, as políticas, ao propiciarem a visibilidade e a participação, permitem o exercício da cidadania, vínculos mais solidários e o enfrentamento do cotidiano na busca das necessidades sociais e em saúde; por outro, elas também podem, ao atuar na contramão da cidadania, contribuir com o imobilismo e clientelismo, (reproduzindo desigualdades. Por fim, muito se tem avançado em termos de políticas públicas; porém, para alcançarmos uma cidadania mais solidária e a saúde, parece ser necessário, por parte do Estado, superar práticas pouco democráticas e, pelo lado da sociedade, um civismo tutelado e conformista.This paper aims to stimulate the debate on the role the State takes in the development of a more supportive citizenship and health promotion. It proposes to analyze those relationships through the Theory of Donation and analytical categories such as

  • Prediction of the potential distribution of Senecio jacobaea in China%新疆千里光Senecio jacobaea在中国的适生区预测

    Institute of Scientific and Technical Information of China (English)

    田忠赛; 徐琳; 程丹丹

    2016-01-01

    [背景]菊科植物新疆千里光原产于欧亚大陆,在北美和澳洲为入侵植物,目前在中国只有新疆有分布记录.新疆千里光一旦成为入侵植物,将对农业、畜牧业和人类健康都可能产生危害,所以需要评估其在中国的扩散趋势.[方法]搜集新疆千里光在中国和世界其他地区的分布记录,结合当前和未来(2050年)气候条件下19种生物气候变量,应用Maxent模型和DivaGis软件,定量预测新疆千里光在中国目前和未来的潜在分布区域;并通过接受者操作特征曲线(ROC)分析法对模型进行精度检验.另外,通过Maxent给出新疆千里光在欧洲(原产地)、北美洲和大洋洲(入侵地)以及中国等4个分布区的年均温度和年均降水量的气候阈值.[结果]用中国和全球分布的数据预测的结果有些差异.前者结果表明除了新疆地区,其他省份几乎没有新疆千里光的适生区;而后者显示在中国其他几个省份也有可能分布,且在甘肃四川交界处有较高适生性.前者模型精确度较高,但2个结果都显示新疆千里光在中国目前和未来的分布区大部分还是在新疆地区.中国分布区年均温度和年均降水量的阈值比其他2个地区都低.[结论与意义]新疆千里光在当前和未来气候条件下在中国未来的扩散趋势较弱,基本局限于新疆地区.用中国分布数据预测优于全球分布数据预测结果,新疆千里光不同分布区的气候阈值的差异揭示分布于中国的新疆千里光与其他地区的种群的生态位有所不同,可能是一个新的亚种,希望未来能进行进一步的研究.

  • Patrimônio de Fé e Religiosidade: os Bens Culturais inseridos na Festa do Senhor dos Passos em São Cristóvão, Sergipe, Brasil (Heritage of Faith and Religiosity: Cultural Objects inserted on the Festival of Senhor dos Passos in São Cristóvão

    Directory of Open Access Journals (Sweden)

    Ivan Rêgo Aragão

    2013-09-01

    Full Text Available Patrimônio de Fé e Religiosidade: os Bens Culturais inseridos na Festa do Senhor dos Passos em São Cristóvão, Sergipe, Brasil. (Heritage of Faith and Religiosity: Cultural Objects inserted on the Festival of Senhor dos Passos in São Cristóvão - DOI: 10.5752/P.2175-5841.2013v11n31p1018O conceito de Bens Culturais de uma localidade vem passando por novos paradigmas quanto à abrangência e relevância. Nesse âmbito, além do Patrimônio Material de ruas, praças, igrejas, objetos artísticos e históricos, o discurso e ações dos órgãos oficiais e da comunidade, devem também se pautar pela valorização das produções imateriais da cultura. Esses elementos envolvem os sujeitos e os sons produzidos por eles, seus ofícios, memórias, festas e religiosidade. Nesse sentido, o presente artigo visa analisar os Bens Culturais que compõem o cenário da celebração do Senhor dos Passos e descrever os seus valores histórico, religioso e artístico. A metodologia utilizada foi: pesquisa bibliográfica, documental e de campo. Após as três etapas da pesquisa e observação no local, constatado que a Festa do Senhor dos Passos em São Cristóvão possui elementos patrimoniais conectados que a transforma em um evento vinculado à identidade cultural-religiosa da região. Alguns desses elementos já são tombados nos níveis estadual, nacional e mundial, enquanto que outros, embora não reconhecidos pelos órgãos oficiais de salvaguarda do Patrimônio, são valorizados pela comunidade no que concerne a afetividade, memória, dádiva e pertencimento: Patrimônios de Fé, Religiosidade e Culto em Sergipe. Palavras-chave: Patrimônio Cultural. Festa. Religiosidade. Senhor dos Passos. São Cristóvão (Sergipe.   Abstract The concept of cultural property that belongs to one locality has been going through new paradigms regarding the scope and relevance. In this context, in addition to material assets (streets, squares, churches and historical

  • Direito humano à alimentação adequada: um olhar urbano The human right to adequate food: an urban vision

    Directory of Open Access Journals (Sweden)

    Juliana Pereira Casemiro

    2010-07-01

    Full Text Available O direito humano à alimentação adequada é compreendido em duas dimensões: estar livre da fome e da desnutrição e ter acesso a uma alimentação adequada1,2. No contexto urbano, com seu forte apelo ao consumo e em que a aquisição de alimentos se dá prioritariamente a partir de relações mercantis, debater este tema em bairros empobrecidos apresenta-se como grande desafio. Este artigo reúne reflexões realizadas a partir de um estudo qualitativo realizado em São João de Meriti, município localizado na Baixada Fluminense (RJ, reunindo líderes da Pastoral da Criança em sessões de grupo focal. O desemprego e subemprego e a dificuldade de acesso aos serviços públicos de saúde, assistência social e saneamento foram apresentados como principais obstáculos para a efetivação do direito humano à alimentação. Foi possível identificar que, dentre as estratégias de enfrentamento da pobreza e da fome, o estabelecimento de circuitos de ajuda mútua tem grande destaque. Apoio social, dádiva e religiosidade apresentaram-se como categorias relevantes nas reflexões dos líderes. Diante de uma realidade em que a pobreza e a fome ou são naturalizadas ou se revertem em moeda de troca durante as eleições, a questão do clientelismo aparece como grande preocupação e desafio para estes líderes.The human right to adequate food is comprehended in two dimensions: being free of hunger and denutrition and having access to an adequate food. The urban context, in which the possession of food is done primarily through merchandising because of its strong consuming appealing, became a big challenge to debate this topic in poor districts today. Here we combine considerations of a qualitative study carried out in São João de Meriti, Rio de Janeiro State, joining leaders from Pastoral da Criança in focal group sessions. The unemployment, the sub-employment and the difficulty in reaching the public health system, the social assistance and basic

  • Centralidade do dinheiro na espiritualidade neopentecostal

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    Drance Elias da Silva

    2009-05-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4  O presente Artigo tem como centro de sua reflexão a espiritualidade centrada no dinheiro. O referido objeto é “ferramenta de Deus” presente nos cultos diários das igrejas neopentecostais. E como tal compõe o arsenal das coisas ofertadas em altar. É símbolo religioso a costurar a relação vertical Deus-igreja-fiel. Mas, não está selecionado como objeto qualquer a tomar parte, como mediação, dessa relação. O dinheiro não é danação ou algo considerado como tal, mas é santificado, quando imolado para se tornar oferta e, assim, contribuir para multiplicar as obras de Deus. Alguns textos – e contextos – tenderiam a afirmar que a penetração do dinheiro, no campo do sagrado, seria para profaná-lo, destruí-lo. Seria verdadeiro? O dízimo é dinheiro, é algo que pertence a Deus. Percebe-se uma mística que se desenvolve a partir de um processo constante de ressignificação. A perspectiva simbólica compõe a análise compreensiva desse objeto chamado dinheiro que, no espaço de culto neopentecostal, ganha importância e expressividade na fé dos que buscam vitória, conquista, prosperidade.  PALAVRAS-CHAVES: Espiritualidade; Dinheiro; Neopentecostalismo; Vinculo social; Dádiva.        Abstract This Article is centered, in its reflection, SPIRITUALITY CENTRALIZED IN MONEY. The above-mentioned object money is an instrument in god’s hands, present in Neo-pentecostal churches daily cult. And in such a perspective, it – money – coumponds things arsenal, offered at altar. It is a religious symbol fitting  to sew GOD – CHURCH – FAITHFUL vertical rapport . But it is not selected as any object at all in order to take part, as a kind of mediation, in this rapport. Money is not a curse, but, sanctified, when immolated to be offered and to multiply the work of God. Some would tend to say that, the entry into the money into the space of the sacred would be to

  • Development and Experimental Study of Phantoms for Mapping Skin Chromophores

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    Silapetere A.

    2014-06-01

    Full Text Available Mūsdienās multispektrālās attēlošanas iekārtas izmanto ādas parametru un fizioloģisko procesu aprakstīšanai gan pētniecības, gan diagnostikas nolūkiem. Iekārtu darbības uzlabošanai ir nepieciešams labāk saprast gaismas mijiedarbību ar audiem, kā arī veikt šo iekārtu kalibrēšanu ar ādas maketu. Redzamā un tuvā infrasarkanā optiskā diapazona spektroskopijā ir svarīgi ādas maketi, kas simulē audu slāņaino struktūru un ķīmiskās īpašības, kā arī maketi, kas ir bioloģiski līdzvērtīgi. Šajā pētījumā tika izveidots ādas makets no bioloģiskām un ķīmiski sintezētām struktūrām. Ādas maketa izveidei tika izmantota fibrīna matrica ar dermālo un epidermālo šūnu piejaukumu, lai imitētu ādas slāņaino struktūru. Fibrīna matrica tiek veidota no 0,47 ml asins plazmas, 0,4 ml fizioloģiskā šķīduma, 0,8 μl treneksāmskābes un 89,4 μl kalcija glukanāta. Izveidoto matricu ievieto šūnu inkubatorā, lai tā polimerizētos. Nākošais slānis tiek veidots ar dermālo šūnu piejaukumu (180-270 šūnas, un pēdējais fibrīna matriksa slānis tiek veidots ar epidermālo šūnu piejaukumu (270 šūnas un šūnu augšanu veicinošu serumu (FBS. Šūnu kultivēšanai nepieciešamas vismaz divas nedēļas. Šajā slāņainajā struktūrā ir iespējams pievienot ādas hromoforu simulējošus iekļāvumus. Optiskajā diapazonā no 450-900 nm ādas hromoforas, kurām ir visizteiktākais spektrs, ir bilirubīns, melanīns un hemoglobīns. Lai simulētu ādas hromoforu spektrālās īpašības, tika izmantots sintezēts bilirubīns, eritrocītu masa un nigrozīns. Lai izpētītu šī maketa iekārtu kalibrēšanas potenciālu, tika izveidoti 76 paraugi, kur katros 24 paraugos bija pievienots viens no absorbentiem ar dažādām koncentrācijām. Pilna ādas maketa audzēšanai nepieciešamas divas nedēļas, lai ātrāk tiktu iegūti pirmie rezultāti tika veidoti maketi bez dermālo un epiderm

  • Development and Experimental Study of Phantoms for Mapping Skin Chromophores

    Science.gov (United States)

    Silapetere, A.; Spigulis, J.; Saknite, I.

    2014-06-01

    šanu veicinošu serumu (FBS). Šūnu kultivēšanai nepieciešamas vismaz divas nedēļas. Šajā slāņainajā struktūrā ir iespējams pievienot ādas hromoforu simulējošus iekļāvumus. Optiskajā diapazonā no 450-900 nm ādas hromoforas, kurām ir visizteiktākais spektrs, ir bilirubīns, melanīns un hemoglobīns. Lai simulētu ādas hromoforu spektrālās īpašības, tika izmantots sintezēts bilirubīns, eritrocītu masa un nigrozīns. Lai izpētītu šī maketa iekārtu kalibrēšanas potenciālu, tika izveidoti 76 paraugi, kur katros 24 paraugos bija pievienots viens no absorbentiem ar dažādām koncentrācijām. Pilna ādas maketa audzēšanai nepieciešamas divas nedēļas, lai ātrāk tiktu iegūti pirmie rezultāti tika veidoti maketi bez dermālo un epidermālo šūnu piejaukuma. Fibrīna matricas un ādas imitējošā maketa absorbcijas spējas ir mazas salīdzinājumā ar hromoforu absorbcijas spējām. Lai novērtētu maketu, kas paredzēti konkrētu hromoforu spektrālo īpašību imitēšanai, iespējams veikt eksperimentus ar fibrīna matricu, kuras izveidošanai ir nepieciešama viena diena. Sintezētā bilirubīna koncentrācijas tika mainītas robežās no 0,01-2,00 mg/ml, melanīna optisko īpašību simulējošās vielas nigrozīna koncentrācija tika mainīta no 1,5 - 312,8 μg/ml, eritrocītu masas koncentrācija mainījās no 0,2 - 42,4 mg/ml.Mērījumi tika veikti, izmantojot multispektrālās attēlošanas iekārtu Cri Nuance 2.4. (Cambridge Research & Instrumentation, Inc., Amerikas Savienotās Valstis). Absorbcijas spektrs tika apstrādāts, izmantojot Microsoft Office Excel 2007. Iegūtajos rezultātos ir iespējams redzēt, ka piedāvātais ādas makets spēj simulēt ādas optiskās īpašības. Izmantotie absorbenti - sintezētais bilirubīns, nigrozīns un eritrocītu masa - spēj simulēt ādas hromoforu spektrālās īpašības. Palielinot absorbentu koncentrāciju paraugā, palielinās absorbcijas spektra maksimālā intensit

  • Coastal vulnerability: climate change and natural hazards perspectives

    Science.gov (United States)

    Romieu, E.; Vinchon, C.

    2009-04-01

    Introduction Studying coastal zones as a territorial concept (Integrated coastal zone management) is an essential issue for managers, as they have to consider many different topics (natural hazards, resources management, tourism, climate change…). The recent approach in terms of "coastal vulnerability" studies (since the 90's) is the main tool used nowadays to help them in evaluating impacts of natural hazards on coastal zones, specially considering climate change. This present communication aims to highlight the difficulties in integrating this concept in risk analysis as it is usually practiced in natural hazards sciences. 1) Coastal vulnerability as a recent issue The concept of coastal vulnerability mainly appears in the International panel on climate change works of 1992 (IPCC. 2001), where it is presented as essential for climate change adaptation. The concept has been defined by a common methodology which proposes the assessment of seven indicators, in regards to a sea level rise of 1m in 2100: people affected, people at risk, capital value at loss, land at loss, wetland at loss, potential adaptation costs, people at risk assuming this adaptation. Many national assessments have been implemented (Nicholls, et al. 1995) and a global assessment was proposed for three indicators (Nicholls, et al. 1999). The DINAS-Coast project reuses this methodology to produce the DIVA-tool for coastal managers (Vafeidis, et al. 2004). Besides, many other methodologies for national or regional coastal vulnerability assessments have been developed (review by (UNFCCC. 2008). The use of aggregated vulnerability indicators (including geomorphology, hydrodynamics, climate change…) is widespread: the USGS coastal vulnerability index is used worldwide and was completed by a social vulnerability index (Boruff, et al. 2005). Those index-based methods propose a vulnerability mapping which visualise indicators of erosion, submersion and/or socio economic sensibility in coastal zones

  • Presentación del Libro: “Los Versos Melánicos”

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    Efraim Otero Ruiz

    2004-03-01

    después de que úno se ha desvelado 4 o 5 años para escribir y lograr que le publiquen un libro, se encuentra a los amigos en las calles o en las reuniones y de golpe le salen, peyorativamente, con esta frase : “- Ala, por ahí vi tu librito…Cuándo me lo mandas?”

    Por eso me he curado en salud con el “Envío”, que aparece en la 6a. página:

    Lector:

    Hay quienes raudos y afanosos un libro escriben, último o primero, y después nos lo envían generosos dedicado con rasgos elogiosos… y regresan al mes por su dinero.

    Esto, que entre poetas fue frecuente, hoy afecta a abogados y a galenos. Se argumenta que, al menos, los gastos de edición son muy copiosos y que el autor, privado de repente de unos cuantos pesitos onerosos y enfrentado del agio a los venenos, debe enmendar su gesto decadente reprimiendo sus gestos dadivosos.

    No para tí, lector: esto es regalo, dádiva, donación, traspaso o beca. Por eso, sin gastarte ni una mueca si te parece malo ya lo podrás tirar a la caneca! Sólo recuerda: escribo estas estanzas con el ánimo austero de compartir contigo mis andanzas y dedicarte algunas remembranzas con el saludo de Efraim Otero.

    Antes de seguir adelante quiero dar gracias anticipadas al amigo y brillante Académico Alberto Gómez por su comentario y agradecer sobre todo a los prologuistas- epiloguistas, Académicos Humberto Roselli y Guillermo Sánchez Medina, a la Academia, a todos los que en una u otra forma ayudaron a sacar este libro adelante, a Martha, a Susanita, a Mauricio Pérez y señora –que diseñaron la bella carátula- a mi hija Pilar, que dibujó las ilustraciones y a la Editorial Kimpres, por haber sacado en tiempo récord una bonita edición con sólo una errata, casi imperceptible en la página 72 y así evitar la crítica que lanzaba el inmortal Luis Donoso en una de sus “charlas” a los tipógrafos

  • Determination of Two-Liquid Mixture Composition by Assessing Dielectric Parameters 1. Precise Measuring System / Divu Šķidrumu Maisījuma Sastāva Noteikšana, Izvērtējot to Dielektriskos Parametrus 1. Precīza Mērīšanas Sistēma

    Science.gov (United States)

    Vilitis, O.; Shipkovs, P.; Merkulovs, D.

    2013-08-01

    ,01%. Šajā darbā piedāvātajā jaunajā mērīšanas sistēmas struktūras risinājumā iekļautas divas tiešas signālu digitālās sintēzes mikrosistēmas, kas ar augstu precizitāti ģenerē mērīšanai nepieciešamās formas, fāzes un amplitūdas signālus ar 100,000 kHz frekvenci un tiek vadītas no mikrokontrolera. Mērīšanas sistēmā izmantota mērsignālu sinhronizētās detektēšanas tehnika. Izstrādātās sistēmas konigurācija, mērīšanas režīma uzstādījums un vadība automātiski praktiski izslēdz virkni mērīšanas signālu parazītisko fāzes nobīžu un fāzes uzstādījumu kļūdas. Izstrādātā mērīšanas sistēma ērti piemērojama vispārējai izmantošanai kapacitātes vai impedances mērierīcēs. Samērā nelielā prognozējamā mērīšanas sistēmas pašizmaksa un kompaktā, portatīvā konstrukcija, kā arī iepriekš minētās priekšrocības paplašina sistēmas lietojumu ne tikai etanola koncentrācijas mērīanai bioetanola ražošanas procesā, bet šī sistēma var tikt izmantota arī eļļas, benzīna un citu degvielu testēšanai, ķīmiskajā, farmācijas un pārtikas industrijā, mikrobioloģijā un medicīnā, lai noteiktu glikozes, antigēnu, antivielu, proteīna, DNS fragmentu un citu vielu saturu šķīdumos u.c.

  • New Species New subspecies and New Record of Butterflies (Lepidoptera) from China, Ⅲ%中国蝴蝶新种、新亚种及新记录种(Ⅲ) (鳞翅目)

    Institute of Scientific and Technical Information of China (English)

    周尧; 张雅林; 王应伦

    2001-01-01

    记述中国粉蝶科1新种、4新亚种、1新型、1中国新记录种及6个《中国蝶类志》未记述的已知种。所有模式标本保存在西北农林科技大学昆虫博物馆。%In the present paper, 1 new species, 4 new subspecies, 1 new form and 1 new record species of Pieridae from China, and reports 6 known species, these were not described in 《Monographia Rhopalocerorum Sinensium》.   All type specimens are kept in the Entomological Museum Northwestern Sci-Tech University of Agriculture and Forestry.    Pieridae 1. Colias aurora Esper f. chloe Eversman, (Figs.1,2)   Obs. specimen: 1♀, Pingliang, Gansu, 15-Ⅶ-1992, col. Wang Huanran. Not described in 《Monogr. Rhop. Sin.》. 2. Colias felderi Grum-Grshimailo, (Figs.3,4)   Obs. specimen: 1♀, Xining, Qinghai, Ⅷ-1990, col. Xu Weibang. Not described in 《Monogr. Rhop. Sin.》. 3. Colias diva Grum-Grshmailo, (Figs.5~8)   Obs. specimens: 1♂, Li Xian, Sichuan, 03-Ⅶ-1997, col. Zhang Yazhou; 1♀, Qilianshan, Gansu, Ⅶ-1989, col. Tian Jianbei; 1♀, Xinglongshan, Gansu, 01-Ⅷ-1993, col. Zhang Yuanling. Not described in 《Monogr. Rhop. Sin.》. 4. Colias sifanica qinghaiensis, ssp. nov. (Figs.9~12)   This subspecies distinguished from typical subspecies by 2/3 basal part of forewing of male black, only yellowish in wing-cells; the black outer band of forewing of femile arc-form, without dear angulated.   Holotype: 1♂, Xining, Qinghai, 1996, col. Xu Weibang; 1♀, Xiahe, Qinghal, Ⅷ-1991, col. Xu Weibang. 5. Prioneris testylis fajiansis, ssp. nov. (Figs.13,14)   This subspecies distinguished from typical subspecies and ssp. formosana Fruhstorfer by following characters: the Cu of hindwing withou black macule, with yallow macule on anal zone, the yellow macules in the cells on under surface of hindwing isolated, with white margine.   Hototype. 1♂, Quanzhou, Fujian, col. Huang Bingwen. 6. Appias nero hainanensis (Felder et Felder), (Figs.15

  • Astronomers Use X-Rays To Probe Gravitational Field Of A Neutron Star

    Science.gov (United States)

    2002-06-01

    more than three decades, their exact nature is still unknown. "We are not even sure that neutron stars are composed of neutrons," said Divas Sanwal, also of Penn State, and lead author on a paper describing the team's results. "They could be largely composed of subatomic particles called pions or kaons, or even free quarks." One key to narrow the range of possibilities is to measure the strength of gravity on the surface of a neutron star by observing its effect on X-rays from very near the surface of the star. According to Einstein's theory of General Relativity, attraction of photons by a star's gravitational field results in a lower energy of the photon (longer wavelength of radiation) when detected by a distant observer. The measurement of this gravitational redshift relates the mass to the radius of the star, and it will test the theories for the various possible forms of dense matter. The team, which also includes Slava Zavlin of Max Plank Institute for Extraterrestrial Physics, Germany, and Marcus Teter of Penn State, considered several possible explanations for the absorption features observed from 1E 1207. The strength and X-ray energy of the features make it improbable that they are due to intervening interstellar material or absorption due to electrons or ions circling in a strong magnetic field. The most likely hypothesis, they conclude, is that the features are due to absorption by helium ions in a magnetic field about a hundred trillion times more intense than the Earth's magnetic field. In this case, the gravitational redshift reduces the energy of the X-rays by 17 percent. Pavlov and his colleagues observed 1E 1027 with Chandra's Advanced CCD Imaging Spectrometer on January 6, 2000, and again on January 5, 2002, each time for approximately 30,000 seconds. The ACIS instrument was built for NASA by Penn State and the Massachusetts Institute of Technology, Cambridge, Mass. under the leadership of Gordon Garmire of Penn State. NASA's Marshall Space Flight

  • Izplatītākie zemnieku vārdi Vidzemē 17. gadsimta pirmajā pusē

    Directory of Open Access Journals (Sweden)

    Renāte Siliņa-Piņķe

    2014-11-01

    Full Text Available Rakstā tiek analizēti 1638. gada zviedru arklu revīzijā fiksētie latviešu zemnieku personvārdi. Raksta mērķis ir noskaidrot izplatītākos vārdus un veikt to analīzi; kā arī balstoties analīzē izdarīt secinājumus par personvārdu pieraksta specifiku avotā un tā iespējamo lomu turpmākā latviešu personvārdu vēstures izpētē. Analīzes procesā vārdi ir salīdzināti ar atbilstošā laika vācu personvārdiem, ņemot vērā gan to pierakstu vācu avotos, gan iespējamās adaptācijas pazīmes revīzijas dokumentos. Pavisam ir saskaitīti 11 995 latviešu zemnieki, minot 829 vārdu variantus. Revīzijas protokolsir rakstīts agrīnajā jaunaugšvācu valodā, un tajā parādās iespējamās latviešu izrunas un vāciskā pieraksta atšķirības dažādos līmeņos: no vācu pilnajām un saīsinātajām formām – kā Heinrich, Wilhelm, Tieß u. c. – līdz pat skaidri saskatāmiem latviskojumiem kā Andrens, Brentzitis, Erman u. c. Tāpat ir fiksētas, iespējams, kļūdaini pierakstītas un nesaprotamas formas, piemēram, Dohße, Lagingz, Peyte u. c. Šo apstākļu dēļ vārdi analīzei tika grupēti, vienā grupā iekļaujot:1 vācu personvārdu vārdnīcās minētos vārda rakstības variantus,2 adaptētās formas ar dažādām latviešu valodas izskaņām,3 avotā fiksētos alonomus,4 rakstvežu nekonsekventās rakstības vai iespējamās pārrakstīšanās un neuzmanības kļūdas.Padziļināti ir analizētas desmit izplatītāko priekšvārdu grupas – Hans (1377 personas jeb 11,5% no visiem minētajiem zemniekiem, Jahn (1194, 10%, Peter (985, 8,2%, Jacob (780, 6,5%, Andres (631, 5,3%, Jurgen (564, 4,7%, Matz (548, 4,6%, Thomas (497, 4,1%, Hinrich (489, 4%, Michell (442, 3,7%. Katra grupa ir nosaukta pēc visizplatītākās vārda formas. Šajās desmit grupās ietvertie 7507 priekšvārdi veido gandrīz divas trešdaļas (62,6 % no visiem revīzijas protokolā minētajiem zemnieku vārdiem. Vārdu pieraksta zi

  • Receiving And Data Acquisition Systems Of Rt-32 For Vlbi Observations / Rt-32 Uztveršanas Un Datu Reģistrācijas Sistēmas Vlbi Novērojumiem

    Science.gov (United States)

    Bezrukovs, Vl.; Shmeld, I.; Nechaeva, M.; Trokss, J.; Bezrukovs, D.; Klapers, M.; Berzins, A.; Lesins, A.; Dugin, N.

    2012-12-01

    ādiem kā Eiropas VLBI tīkls, Zemo frekvenču VLBI tīkls (LFVN) un citiem. Pēdējos gados rekonstruēta teleskopa sekundārajā fokusā izvietotā uztvērēju telpa un tajā uzstādīti vairāki jauni uztvērēji. Pašreiz radioteleskops ļauj veikt novērojumus četros viļņu garumu diapazonos: 92 cm, 18 cm, 6 cm un 2.5 cm. No minētajiem pirmie 3 jau tiek veiksmīgi izmantoti dažādos VLBI eksperimentos. 2.5 cm uztvērējam ir tikai viens lineārās polarizācijas kanāls, kuru izmanto galvenokārt metanola māzeru novērojumiem viena teleskopa režīmā. RT-32 aparatūru veido divas neatkarīgas VLBI datu reģistrācijas sistēmas: TN-16 un DBBC kopā ar Mark5b. Abas sistēmas izmanto interferometriskajos novērojumos atkarībā no eksperimentu mērķa un radioteleskopa iespējām. Aplūkots Irbenes RT-32 radioteleskopa pašreizējais statuss, tā VLBI novērojumiem piemērotās uztveršanas un datu reģistrācijas iekārtas, kā arī notikušajās VLBI sesijās uzkrātā pieredze.

  • Fungos Presentes em Acículas de Pinus taeda em Estágios Iniciais de Decomposição no Campo Fungi Present on Pinus taeda Needles in Early Stages of Decomposition in the Field

    Directory of Open Access Journals (Sweden)

    Angela Michelato Ghizelini

    2011-03-01

    Full Text Available <div>A atividade da micobiota na decomposição e mineralização da matéria orgânica é importante na ciclagem de nutrientes em florestas, garantindo sua produtividade e sustentabilidade. Este estudo determinou a diversidade de fungos durante a decomposição de acículas de Pinus taeda, em um plantio experimental com quatro anos de idade, em Três Barras, SC, Brasil. Acículas senescentes foram coletadas em árvores em novembro de 2003 e colocadas em sacolas seletivas para  microrganismos e deixadas sobre a serapilheira da floresta. O isolamento de fungos foi feito das acículas da primeira coleta (novembro de 2003 e das mantidas em sacolas, nos meses de fevereiro, maio e agosto de 2004. Fragmentos de acículas foram submetidos a 20 lavagens sucessivas em água destilada estéril e implantados em placas de petri contendo meio extrato de malte 2 %. As colônias encontradas foram purificadas, identificadas e preservadas, perfazendo um total de 1.055, pertencentes a 13 fungos: Acremonium sp., lternaria sp., Cladosporium sp., Colletotrichum p., picoccum sp., Fusarium sp., liocladium sp., Mucor sp., Penicillium sp., Pestalotia sp., Rhizoctonia sp., Trichoderma sp. e Verticillium sp. Os fungos Trichoderma sp., Fusarium sp. e Verticillium sp. foram significativamente mais freqüentes. Considerou-se que a diversidade fúngica existente é suficiente para o início da decomposição das acículas, pela presença de fungos celulolíticos.

  • Knowing of the dependence between local area productivity, nutrient cycling and litter decomposition process, the knowledge of the mycobiota responsible for decomposition is the right way to obtain answers about forest’s productivity and nutrient’s demand. This study determined the fungal diversity during litter decomposition of needle

  • BACTERIOLOGICAL CHARACTERISTICS OF WASHED AND UNWASHED LAYING HEN’S EGGS CARACTERÍSTICAS BACTERIOLÓGICAS DE OVOS LAVADOS E NÃO LAVADOS DE GRANJAS DE PRODUÇÃO COMERCIAL

    Directory of Open Access Journals (Sweden)

    Maria Luiza Ferreira Stringhini

    2009-12-01

    causing human intoxication. Although low frequency (2.1%, it was verified the presence of faecal coliforms in number greater than 100 germs/g of eggshell in the classification hall of the farms that washed mechanically the eggs. It was not observe the presence of Salmonella spp. in all the eggshells collected neither contamination in contents of the eggs. It was concluded that washed eggs from commercial egg farms had better eggshell bacteriological quality than unwashed eggs but the washing process adopted was not able to completely eliminate the fecal coliforms.


    KEY WORDS: Commercial eggs, egg washing, food safety, microbiological contamination.

    <div>A avaliação da qualidade bacteriológica de ovos lavados e não lavados de quatro granjas de postura comercial da região metropolitana de Goiânia, GO, constituiu o objetivo do presente estudo. As Granjas 1 e 2 eram de médio e pequeno porte, respectivamente, e realizavam a lavagem mecanizada dos ovos com água aquecida contendo sanitizante; as Granjas 3 e 4 comercializavam ovos não lavados. O universo amostral foi constituído por 576 ovos das Granjas 1 e 2, e 132 ovos obtidos nas Granjas 3 e 4, sendo metade coletada nos galpões e a outra metade na sala de classificação. Avaliaram-se ovos classificados como grandes, de poedeiras leves, com trinta a quarenta semanas de idade, da linhagem Dekalb White. O delineamento utilizado foi inteiramente casualizado em esquema fatorial 2 x 2 (procedimento de lavagem x local da coleta, com três repetições, e a unidade experimental consistiu de pool de seis ovos. Efetuaram-se contagens de mesófilos e Staphylococcus coagulase positivo e número mais provável (NMP de coliformes totais e fecais nas cascas e conteúdos dos ovos e pesquisa de Salmonella spp. nas cascas. Resultados das contagens foram expressos em log UFC/g e aplicou-se o teste “t” de Student (5%. Análises descritiva e de frequência foram adotadas para NMP de