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Sample records for aguacate persea americana

  1. Evaluación del proceso de extracción de aceite de aguacate hass (Persea americana Mill) utilizando tratamiento enzimático

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    Buelvas Salgado, G. A; Patiño Gómez, J. H; Cano-Salazar, J. A

    2012-01-01

    Introducción. El aceite de aguacate es rico en vitaminas A, B, C y E, y está compuesto mayoritariamente por ácidos grasos monoinsaturados los cuales reducen de manera efectiva el nivel de colesterol en la sangre, ayudando en la prevención de enfermedades cardiovasculares. Objetivo. Evaluar el rendimiento de extracción de aceite de aguacate Hass (Persea americana Mill) en estado E3-M (maduro) procedente del Oriente Antioqueño, empleando complejos enzimáticos comerciales. Materiales y métodos. ...

  2. Diversidad de los Hongos Micorrízicos Arbusculares asociados a plantas de aguacate (Persea americana Mill.), en un agroecosistema del Estado de Michoacán.

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    GÓMEZ DORANTES, NÚRIA

    2012-01-01

    Se realizó un estudio para identificar las especies de hongos micorrizógenos arbusculares (HMA) asociados al suelo y al interior de las raíces de plantas de aguacate (Persea americana Mill.) en una huerta denominada las Parrillas en la región aguacatera del Estado de Michoacán, México. Cinco árboles de aguacate establecidos en un diseño completamente al azar fueron muestreados, mostrando altos porcentajes de colonización micorrízica al interior de las raíces (>94%) sin prese...

  3. Consideraciones sobre el cultivo del aguacate Persea Americana Mill. en Atlixco, Puebla

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    Teresa Reyna Trujillo

    1983-01-01

    Full Text Available The production of avocado Persea americana Mill in our country has been intensified the last years, particularly in some states such as Michoacán, Puebla, Veracruz and others. Specifically in Puebla, there are some counties "municipios", Atlixco among others, where its exploitation represents an important economical fact. Because of this, it is being pretended to increase the surface occupied with that fruit tree. However, its growth and cultivation, has been exposed to a great number of problems, such as the ecologic ones (inadecuate soils, for example and fitopathologic ones mainly, reasons enough to consider the increase of its growth as inadecuate.

  4. BIOLOGÍA DE OLIGONYCHUS YOTHERSI (MCGREGOR (ACARI: TETRANYCHIDAE SOBRE AGUACATE PERSEA AMERICANA MILL. CV. LORENA (LAURACEAE

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    Reyes Julian C

    2011-06-01

    Full Text Available Se estudió la biología del ácaro rojo Oligonychus yothersi en condiciones controladas(26 ± 3°C 56 ± 3% HR, sobre hojas maduras de aguacate, Persea americana Mill. cv.Lorena. Las observaciones se realizaron durante una generación del ácaro. La duraciónde todos los estados, a excepción del adulto, fueron los siguientes: huevo 119.06 horas±13.77 (4.96 días; larva 54.09 horas ± 18.48 (2.25 días, protocrisálida 18.22 ± 9.53(0.76 días, protoninfa 50.62 horas ± 13.29 (2.11 días, deutocrisalida 21.66 horas ±7.78 (0.90 días, deutoninfa 62.54 horas ± 12.85 (2.61 días y teliocrisalida 30.79 horas± 13.28 (1.28 días. La duración total de huevo a emergencia de adulto fue estimadaen 344.21 horas (14.34 días. La relación de sexos encontrada fue de 4.8 hembras: 1macho. Los parámetros poblacionales, i.e., longevidad, fecundidad, tasa intrínsecade crecimiento (r m , tiempo de duplicación y tiempo generacional, sugieren un altopotencial reproductivo y de desarrollo del ácaro sobre aguacate cv. Lorena.

  5. ETIQUETAS DE SECUENCIAS EXPRESADAS DIFERENCIALES DE FRUTOS DE AGUACATE RAZA MEXICANA (Persea americana Mill. var. drymifolia

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    Enrique Ignacio Sánchez-González

    2018-02-01

    Full Text Available RESUMEN En este estudio se desarrollaron e identificaron etiquetas de secuencias expresadas diferencialmente (ESTs de frutos inmaduros de Persea americana Mill. var. drymifolia del estado de Nuevo León, México. Diez genotipos con frutos de forma y tamaño diferentes fueron seleccionados para generar ESTs por la técnica de despliegue diferencial.Se obtuvieron en total 393 fragmentos diferenciales amplificados, 82 fragmentos diferenciales fueron secuenciados y editados para identificación y comparación en las bases de datos para nucleótidos y proteínas del NCBI. Cuarenta secuencias mostraron similitud significativa con secuencias de ARNm y/o secuencias de proteínas hipotéticas o predichas pertenecientes a P. americana y/o a otros géneros. Algunas secuencias estuvieron relacionadas a enzimas como flavanona-3-hidroxilasa (F3H, lecitina-colesterol aciltransferasa, glutatión-S-transferasamicrosomal y proteína pleiotrópica de resistencia a drogas. Con la información de la composición nucleotídica de las ESTs se podrán diseñar iniciadores para cuantificar niveles de expresión por RT-PCR en tiempo real de los genes, en diferentes etapas fenológicas del fruto y hacer comparaciones entre los genotipos que permitan determinar usos alternos de sus frutos.

  6. Caracterización de algunos rasgos vegetativos y reproductivos en variedades de aguacate, Persea americana Mill.

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    Escandón Gil Gonzalo

    1989-12-01

    Full Text Available En Palmira (Colombia entre 1984 y 1988 se caracterizó la colección de aguacate, Persia americana Mili. Se halló la ecuación de regresión AF = 89.25 + 0.60 (La -144, R2= 98.58 %, que permite estimar el área foliar (AF con el producto largo por ancho de las hojas (A. Las variedades Mayapan, Oriente 1, Oriente 3, Simonds y los materiales 2, 3, 4, 5 y 6 presentaron épocas de floración definidas, Trinidad y Material 2 presentaron floraciones bajas pero continuas. El porcentaje de flores que llegó a frutos osciló entre 0.03 % para Oriente 3 y 0.57 % para Trinidad. La mayor pérdida de estructuras reproductivas ocurrió en los primeros días de floración. El modelo logístico no describió correctamente el desarrollo de los frutos, por esto se utilizó el modelo de línea recta por fases. Los materiales 2 y 5 presentaron 105mayores pesos de frutos (621 y 626 g; el de menor peso fue Booth 8. La pérdida de peso del fruto durante la maduración fluctuó entre 3.5 Y 12.8 % para el material 2 y Oriente 3 respectivamente. Los materiales de mayor aceptación por la presentación del fruto y sabor fueron los materiales 1, 2 y 5.A collection of avocado, Persea americana Mill, was characterized in Palmira (Colombia between1984 and 1988. It was found that regretion equation AF = 89.25 + 0.60 (LA-144, R2 = 98.58 %, allows to estimate the foliar area with the product length x width of the leaves. The Mayapan, Oriente 1, Oriente 3, Simmonds and Materia.1 2,3,4,5,6, varieties showed defined blooming time, Trinidad and Material 2 showed low and continue blooming times. The percentajes of flowers that matured in fruit fluctuated between 0.03 % of Oriente 3 and 0.57 % for Trinidad. The greater loss proportion of reproductive structures occurred during the first days of flowering. The logistic model did not describe the right developments of fruits, that is why a straight line model by stages was used. Material 2 and 5 showed the greatest fruit weights during

  7. RELACIONES GENÉTICAS DEL AGUACATE (Persea americana Mill. EN SIETE MUNICIPIOS DEL CENTRO DE VERACRUZ, CARACTERIZADAS CON MICROSATÉLITES

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    María Elena Galindo-Tovar

    2011-11-01

    Full Text Available México es el primer productor y consumidor de aguacate a nivel mundial. Las condiciones ecotopográficas de la zona Centro del estado de Veracruz y la distribución natural del género Persea, convierten el área en un gran acervo genético del aguacate. Debido a que esta especie presenta un alto grado de hibridación, la evaluación de las relaciones genéticas permite distinguir diferentes taxa e identificar material promisorio para programas de mejoramiento. El objetivo fue analizar las relaciones genéticas de Persea americana en la zona Centro del estado de Veracruz, mediante microsatélites. Se analizaron muestras foliares de 44 árboles ubicados en siete localidades; se realizó extracción de ADN y su amplificación utilizando iniciadores para microsatélites. Los datos se analizaron con el programa PopGene 3.2. Todas las localidades resultaron polimórficas, y aunque mostraron baja diferenciación genética, en el dendrograma se observaron dos grupos definidos por características de altitud, clima y suelo.

  8. Bioprospección de hongos micorrízicos arbusculares como alternativa para el fortalecimiento del cultivo de aguacate (Persea americana Miller en Colombia

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    Sandra Patricia Montenegro Gómez

    2017-06-01

    Full Text Available El Aguacate (Persea americana Miller es uno de los frutos de mayor consumo mundial. México es el principal productor y  actualmente afronta problemas como el ataque de patógenos a la raíz y recientemente se han registrado impactos en la salud humana por uso de agroquímicos. Una alternativa para afrontar problemáticas en el desarrollo de las plantas ha sido iniciar el correcto manejo de las primeras etapas de producción y con ello mitigar problemas posteriores, en este manejo los microorganismos juegan un papel fundamental. Uno de los métodos es la inoculación con microorganismos del suelo como los hongos micorrízicos arbusculares (HMA; diversos estudios han demostrado que su colonización radicular en las plantas contribuye con la absorción de agua y nutrientes; lo cual indica que su potencialización puede contribuir en el manejo sustentable de los cultivos y como opción para fortalecer la producción. Colombia ocupa el quinto lugar en productividad a nivel mundial y lo que más resta competitividad local es el costo de los agroinsumos; en este sentido se podrían fortalecer investigaciones encaminadas a la bioprospección de HMA específicos para diversas variedades de aguacate en las regiones colombianas, ya que son poco los avances en esta temática. Avanzar tecnológicamente en el manejo de cultivos y generar estrategias sostenibles hace parte de las perspectivas y retos de la cadena del aguacate en Colombia. Adicionalmente es prioritario entrar en la dinámica de la demanda mundial con preferencia de alimentos más sanos y cuya producción sea amigable con el medio ambiente.

  9. COMPORTAMIENTO ESPACIAL DE TRIPS (INSECTA: THYSANOPTERA), EN PLANTACIONES COMERCIALES DE AGUACATE (PERSEA AMERICANA MILL.) EN ZITÁCUARO, MICHOACÁN, MÉXICO

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    José Francisco RAMÍREZ DÁVILA

    2013-01-01

    México es el principal productor y exportador de aguacate en el mundo. Los trips son considerados como una de las principales plagas del aguacate en México, debido a su asociación con la enfermedad denominada roña del fruto, causada por el hongo Elsinoe perseae. Esta enfermedad representa una fuerte limitante a la producción del cultivo; el hongo penetra la fruta por las heridas causadas por el complejo de varias especies de trips. El análisis del comportamiento espacial de esta plaga proporc...

  10. ÁCAROS ASOCIADOS AL CULTIVO DEL AGUACATE (Persea americana Mill EN LA COSTA CENTRAL DE PERÚ

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    Jorge Luis Muñoz Marticorena

    2014-01-01

    Full Text Available En la costa central de Perú (Lima e Ica, se cultiva el aguacate con fines de exportación, especialmente la variedad Hass. Con el objetivo de conocer la diversidad de ácaros depredadores en huertos comerciales de aguacate, se realizaron recolectas en 6 lugares de la costa central, duran - te el 2010 al 2012. Se registraron 12 especies de la familia Phytoseiidae, de los cuales algunos tendrían potencial como depredadores eficien - tes de Oligonychus sp . (Guanilo et ál. 2012; estos son: Amblyseius aerialis (Muma, Ambly - seius chungas (Demmark y Muma, Amblyseiella setosa (Muma, Euseius emanus (El–Banhawy, Euseius concordis (Chant, Euseius stipulatus (Athias–Henriot, Phytoseiulus persimilis (Athias Henriot, Phytoseiulus macropilis (Banks, Neo - seiulus californicus (McGregor, Typhlodromina subtropica (Chant, Typhlodromus ( Antoseius evectus (Schusters y Aristadromips n.sp. Se con - firmó que la vegetación espontánea cumple un rol importante en la conservación de las especies halladas en este cultivo y que además la intro - ducción de un ácaro depredador exótico, como Euseius stipulatus, contribuyó significativamente con el control de la plaga durante la temporada de invierno.

  11. SITUACION ACTUAL DEL CULTIVO DEL AGUACATE (Persea americana Mill.) EN EL ESTADO DE MÉXICO, MÉXICO

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    M. Rubí-Arriaga

    2013-01-01

    El objetivo de este trabajo fue determinar la situación actual de la producción y comercialización del cultivo del aguacate en el sur del Estado de México en 2011. El tamaño de muestra se determinó considerando el universo total de inscritos en la base de datos de la SEDAGRO conformada por 2,814 productores. Con base en la metodología de poblaciones finitas, el tamaño de muestra fue 338, a los cuales se les aplicó un cuestionario. La información cuantitativa de la encuesta se capturó e...

  12. El viroide de la mancha del sol (ASBVd es persistente en cultivos nucelares de aguacate (Persea americana Mill.

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    Isidro E Suárez

    2005-07-01

    Full Text Available Con el objetivo de determinar la posibilidad de obtener tejidos sanos a partir de plantas infectadas con ASBVd como una estrategia para recuperar clones de alto valor agronómico, cultivos embriogénicos de aguacate fueron inducidos a partir del nucelo de tres cultivares de aguacate infectados con el viroide de la mancha del sol (ASBVd en un medio de cultivo con las sales mayores B5, suplementado con las sales menores de Murashige y Skoog (MS y 0.41 uM de picloram, y (en mg/L-1 tiamina HCl (0.4, mio-inositol (100, sucrosa (30,000 y TC agar (8,000. Los cultivos embriogénicos inducidos fueron mantenidos en medio semisólido MS suplementado con 0.41 uM de picloram y (en mg/L-1 tiamina HCl (0.4, mio-inositol (100, sucrosa (45,000 y TC agar (8,000 y en medio líquido MS modificado con 15 mM NH4NO3 y 30 mM KNO3, sales menores de MS, picloram 0.41 uM y (en mg/L-1 tiamina HCl (0.4, mio-inositol (100 y sucrosa (45,000. La indexación usando RT-PCR un año después de inducidos los tejidos detectó bandas amplificadas en los electroferogramas y la clonación comprobó la presencia de 4 clones con mas de un 97% de similitud con la variante J02020 de ASBVd; con la secuencia se corroboró la existencia de las variantes CF3, CF8, CF13. Estos resultados demuestran que aunque ASBVd no es eliminado mediante el uso de embriogénesis somática, la aplicación de esta tecnología permite inducir, proliferar y mantener por largo tiempo tejidos infectados para el estudio de este patógeno.

  13. Caracterización química foliar de los árboles de aguacate criollo (Persea americana var. drymifolia en los bancos de germoplasma de Michoacán, México Foliar chemical caracterization of the creole avocado trees (Persea americana var. drymifolia in the germplasm banks from Michoacán, Mexico

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    Cintia A. Rincón-Hernández

    2011-06-01

    Full Text Available Persea americana var. drymifolia (aguacate criollo mexicano se emplea como portainjerto en huertos de aguacate Hass y es fuente de genes de resistencia a plagas y patógenos para éste y otros cultivares. El aguacate criollo mexicano se está sustituyendo por cultivares comerciales o perdiendo por la destrucción de ecosistemas. Por eso, se estudiaron 250 árboles (52 accesiones de 14 estados de México; 247 de criollo mexicano y 3 híbridos de éste con P.shiedeana, P. americana var. americana y P. americana var. guatemalensis, plantados en los bancos de germoplasma en Michoacán. Se analizó la composición química mediante cromatografía de gases-masas. Hubo alta variabilidad química entre los árboles del banco de germoplasma con los 64 compuestos detectados. El análisis de cúmulos mostró 2 grandes grupos químicos con 100% disimilitud y 22 grupos a 10% de disimilitud. Los híbridos se agruparon de manera diferente a los árboles con características típicas de criollo y difieren en su altitud de origen. Corroboramos que los volátiles foliares sirven como marcadores para diferenciar los criollos típicos de sus híbridos y que mucha variación atribuida a la variedad criolla proviene de hibridaciones en zonas de contacto de esta variedad con otras variedades o especies de Persea.Persea americana var. drymifolia (mexican creole avocado is used as rootstock in Hass avocado orchards and it is a source of genes for resistance to pests and pathogens for this and other cultivars. The Mexican varieties are being replaced by commercial cultivars or lost by the destruction of ecosystems. Therefore, we studied 250 avocado trees of 52 accessions from 14 Mexican states; 247 mexican creole avocado and 3 hybrids of that tree with P. shiedeana, P. americana var. americana or P. americana var. guatemalensis, planted at germplasm banks, Michoacán. Foliar chemical composition was analyzed by GC-MS. High chemical variability among the trees was found

  14. Evaluación del proceso de extracción de aceite de aguacate hass (Persea americana Mill) utilizando tratamiento enzimático

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    Buelvas Salgado, Gustavo Andrés; Patiño Gómez, J. H.; Cano Salazar, J. A.

    2012-01-01

    Introduction. Avocado oil is rich in vitamins A, B, C and E, and is mainly composed by monounsaturated fat acids, which effectively reduce cholesterol levels in blood, thus helping to prevent cardiovascular diseases. Objective. Evaluating the performance of Hass avocado (Persea americana Mill) oil extraction in E3-M (mature) from the East of Antioquia, by the use of commercial enzymatic complexes. Materials and methods. To develop this research work, a physical-chem...

  15. Inhibición de la expresión del sistema agr de Staphylococcus aureus resistente a meticilina mediante el uso de polifenoles totales de hojas de aguacate mexicano ( Persea americana var. drymifolia

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    M.A. García-Moreno

    2017-01-01

    Full Text Available Introducción : La resistencia a los antibióticos por parte de las bacterias está provocando gran impacto negativo en la salud humana y en la producción animal. Actualmente, Staphylococcus aureus es considerada dentro de los seis principales grupos bacterianos con mayores reportes de aparición de resistencia a nivel mundial . Por otro lado , durante décadas las plantas de uso medicinal han sido fuente importante de compuestos bactericida s ; sin embargo, también se ha empezado a documentar otras moléculas con otros mecanismos antimicrobianos , como es la inhibici ón de la percepción de quórum ( anti - p ercepción de q u ó rum ( APQ . En la medicina tradicional mexicana las hojas de aguacate mexicano ( Persea americana var drymifolia han sido empleadas para tratar diversos padecimientos que involucran infecciones bacterianas, por lo que es factible contenga compuesto s que pose a n efectos antioxidantes , bactericidas y APQ, que ayuden a combat ir las infecciones de cepas resistentes. De t al manera , que e l objetivo de esta investigación fue determinar el efecto de los polifenoles totales de las hojas de Persea americana var. drymifolia y su actividad APQ sobre cepas de S. aureus resistente s a meticilina (MRSA . Método s : Se obtuvieron l os polifenoles presente s en las hojas de 18 cultivares de aguacate mexicano mediante extracción asistida por ultrasonido y fueron purificados en una columna de amberlita . E stos polifenoles fueron analizados funcionalmente y evaluados contra cepas de S. aureu s MRSA para determinar su efecto APQ sobre el sistema denominado Agr . Resultados : Los polifenoles extraídos de las hojas de aguacate de raza mexicana exhiben una fuerte actividad bactericida . D e los 18 cultivares probados, la variedad María Elena fue la que presentó el mayor efecto , exhibiendo una CMI de 116 μg∙ mL - 1 y un CMB de 133 μg∙ mL - 1 contra la cepa de MRSA μ3 . Sin embargo, también logran inhibir los niveles

  16. Caracterización molecular, análisis morfológico y colonización micorrízica en la rizósfera del aguacate (Persea americana Mill en Caldas, Colombia

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    Fredy Arvey Rivera Páez

    2016-10-01

    Full Text Available Se evaluaron familias de hongos formadores de micorriza arbuscular (HMA asociados a dos variedades de aguacate Persea americana Mill (hass y lorena, en 12 muestras de suelo rizosférico y raíces en Caldas, Colombia. Las esporas se obtuvieron por tamizado húmedo y centrifugación en gradiente de sacarosa, y montadas en los reactivos Polivinilactoglicerol y Melzer para determinar su morfología, y las raíces tratadas con hidróxido de potasio, ácido acético, azul de tripano y lactoglicerol, para establecer su colonización. El ADN fue extraído por DNeasy Plant Mini Kit (QiagenTM y amplificado por PCR utilizando iniciadores sobre los genes 18S y 28S del ADNr nuclear, visualizado y purificado en geles de agarosa, y secuenciado por Macrogen Advancing Through Genomics–Corea del Sur. Los resultados muestran presencia de 12 morfotipos pertenecientes a las familias Acaulosporaceae, Gigasporaceae, Diversisporaceae y Glomeraceae, y una colonización micorrízica superior al 90% en ambas variedades de aguacate. Se logró aislar ADN de nueve morfotipos y amplificar siete. El alineamiento de las secuencias de ADN se realizó en el programa ClustalW incluido en el programa Mega 6, junto con secuencias depositadas en el GenBank, utilizando el parámetro de distancia Kimura 2 (K2P, permitieron construir un árbol de similitud por el método Neighbor-Joining (NJ agrupando las muestras determinadas por morfología en las familias correspondientes. Estos resultados permitieron estandarizar protocolos para familias de este grupo, donde su taxonomía es dificultosa y controversial, registrando familias citadas en la literatura como de elevada importancia para la protección e incorporación de nutrientes en la planta.

  17. Zonificación agroecológica del aguacate (Persea americana Mill. var. Hass) en la cuenca del Río Duero

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    Campos Campos, Oswaldo

    2013-01-01

    El cultivo de aguacate es de gran importancia en México y particularmente en el estado de Michoacán, el cual ocupa el primer lugar a nivel mundial en calidad y producción, este periodo de gran auge por el que está pasando el cultivo, además de traer beneficios, también ha ocasionado un acelerado cambio de uso de suelo forestal a producción de aguacate, al grado de provocar un deterioro en los ecosistemas forestales. En la cuenca del río Duero al formar parte de la zona de la Fr...

  18. Contenido de aceite en frutos de selecciones de aguacate (Persea americana criollo de clima tropical y subtropical de Nayarit, México

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    Raúl Medina Torres

    2017-07-01

    Full Text Available El trabajo se desarrolló sobre 65 selecciones de aguacate criollo de las zonas tropical y subtropical de Nayarit, con el objetivo de calificar el contenido de aceite y relacionarlo con el porcentaje de materia seca y del color de la pulpa del fruto, y de ubicar e identificar selecciones de interés comercial. Se usó análisis estadístico multivariado de componentes principales para el procesamiento de datos. Dos componentes principales explicaron el 63,93 % de la varianza total. En el componente principal 1 resultaron relevantes porcentaje de aceite y materia seca de la pulpa; y en el componente principal 2 las variables de color de la pulpa en madurez de consumo. Cuatro grupos de selecciones fueron identificadas por los promedios de los vectores característicos de los componentes 1 y 2. Se encontró alta correspondencia entre el contenido de aceite y materia seca en la pulpa (R2 = 0,9387 de los aguacates. El color de la pulpa del fruto no resultó un parámetro que se relacione con el contenido de aceite de la pulpa de aguacate. El contenido de materia seca puede constituir un parámetro fácil y útil para determinar con aproximación el contenido de aceite. En general, los aguacates de clima subtropical mostraron mayor contenido de aceite que los de origen tropical.

  19. SITUACION ACTUAL DEL CULTIVO DEL AGUACATE (Persea americana Mill. EN EL ESTADO DE MÉXICO, MÉXICO

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    Martín Rubí Arriaga

    2013-04-01

    Full Text Available El objetivo de este trabajo fue determinar la situación actual de la producción y comercialización del cultivo del aguacate en el sur del Estado de México en 2011. El tamaño de muestra se determinó considerando el universo total de inscritos en la base de datos de la SEDAGRO conformada por 2,814 productores, con base en la metodología de poblaciones finitas, el tamaño de muestra fue 539, a los cuales se les aplicó un cuestionario. La información cuantitativa de la encuesta se capturó en una base de datos y se procesó mediante Microsoft Office Excel 2007©, para obtener medidas de tendencia central. En el Estado de México 85% de la población de estudio, son pequeños productores y tan solo 15% cuentan con extensiones superiores a 10 ha. La producción se basa en una explotación univarietal con predominio del 90% de la variedad Hass. El incremento en plantaciones de aguacate se ha realizado en 77% con planta proveniente del estado de Michoacán. La producción se destina para el consumo nacional, se comercializa principalmente en las centrales de abasto de Toluca, Estado de México, Distrito Federal y en mercados regionales. Aguacate Hass; producción; comercialización; consumo;

  20. RELATIONSHIP OF Monalonion velezangeli CARVALHO & COSTA (HEMIPTERA: MIRIDAE WITH THE PHENOLOGY OF AVOCADO (Persea americana MILL., CV. HASS RELACIÓN DE Monalonion velezangeli CARVALHO & COSTA (HEMIPTERA: MIRIDAE CON LA FENOLOGÍA DEL AGUACATE (Persea americana MILL., CV. HASS

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    Luisa Fernanda Torres Jaimes

    2012-06-01

    Full Text Available Abstract. Monalonion velezangeli is considered one of the most harmful pests of avocado cv. Hass, as far as it attacks vegetative buds, flowers and fruits during all the productive stages of the crop. This situation is aggravated by the lack of knowledge on insect preferences and their relationship with crop phenology. As a contribution to the management of this insect, we studied the relationship between plant phenology and the presence of M. velezangeli, as well as its preferences across tree strata and structures. Data were obtained from six orchards located in the Colombian departments of Antioquia, Caldas and Quindío. After randomly selecting 20 trees at each orchard, 12 branches were marked on each tree, covering the combinations of the four cardinal points with three plant strata (low, medium and high. During a period of 12 months, each branch was monitored by recording its phenological stage (vegetative, flowering and fruiting, the number of individuals of M. velezangeli and the presence of fresh damages on it. An analysis of variance determined that the presence of the insect and its damage were more abundant on the flowering and fruiting branches than on the vegetative ones, as well as in the medium and upper strata of the trees. The damage caused by M. velezangeli was observed to be significantly higher in the reproductive structures (flowers and fruits as compared to the vegetative structures.Resumen. Monalonion velezangeli es considerada una de las principales plagas del aguacate cv. Hass en Colombia, debido a que ataca brotes vegetativos, flores y frutos, durante todas las etapas productivas del cultivo. Esta situación se ve agravada por el desconocimiento de las preferencias del insecto, así como de su relación con la fenología de la planta. Como un aporte al manejo de este insecto, se estudió la relación entre la fenología de la planta y la presencia de Monalonion, así como sus preferencias por estructura y estrato. Las

  1. Potencial del cultivo de aguacate (persea americana mill en Ecuador como alternativa de comercialización en el mercado local e internacional

    Directory of Open Access Journals (Sweden)

    Andrea Sotomayor

    2016-12-01

    Full Text Available El Ecuador, por su ubicación geográfica posee las condiciones climáticas adecuadas para el cultivo del aguacate, siendo las principales zonas productoras: Carchi, Imbabura, Pichincha, Tungurahua, Azuay y Loja. La variedad demandada a nivel internacional es la ‘Hass’; sin embargo en el mercado local es la aceptación es por la variedad ‘Fuerte’. Entre los principales países importadores y potenciales mercados de este frutal tenemos Estados Unidos, Francia, Holanda, Japón, entre otros. No obstante, las exportaciones de aguacate ecuatoriano han sido principalmente a Colombia (99 %, alcanzando un valor de 2.2 millones de dólares americanos; y en menor escala a las Antillas Holandesas, España y Holanda. El precio en el mercado local varía entre 1.06 y 1.90USD/kg, observándose inestabilidad; sin embargo, en el mercado europeo alcanza un valor de 3.60 USD/kg constituyéndose una alternativa atractiva para su comercialización. En el mercado local no existe una demanda insatisfecha debido a que la producción nacional se la comercializa casi en su totalidad en el país; mientras que existe una demanda insatisfecha a nivel internacional que constituye una oportunidad de mercado. Abstract Ecuador has adequate weather conditions for growing avocado due to its geographic location. The main producing areas are Carchi, Imbabura, Pichincha, Tungurahua, Azuay and Loja. ‘Hass’is the variety demanded in the international market; however the variety ‘Fuerte’ is the most consumed in the local market.Among the major importing countries and potential markets for this fruit are United States of America, France, Holland, Japan, among other.However, the Ecuadorian avocado exportation has been mainly to Colombia (99 %, reaching an amount of 2.2 million of American dollars; and a low scale to the Netherlands Antilles, Spain and Holland.The price in the local market varies between 1.06 and 1.90USD/kg, resulting in instability; on the other hand, in

  2. Physicochemical Properties of Avocado Pear (Persea americana ...

    African Journals Online (AJOL)

    The oil from edible avocado pear (Persea americana) was extracted using the Soxhlet extraction method and characterized for specific gravity, refractive index, free fatty acids, saponification value, iodine value, acid value and biofuel potential using standard methods. The percent oil content in the fruit pulp was determined.

  3. Biologi Perbungaan Tanaman Avokad (Persea Americana Mill.)

    OpenAIRE

    SUKAMTO, L. AGUS

    1985-01-01

    L. AGUS SUKAMTO 1985.The flowering biology of avocado (Persea americana Mill.). Berita Biologi 3 (1) 8 - H.- A study on the flowering biology of avocado available at Bogor Botanic Garden was made.The observation was conducted in respects to the opening and closing of the flowers, the versality of the pollen grains which are then related to the fruit production.The climatic condition as well as pollination agents which may affect on the vertilization in avocado are also discussed.

  4. The Scirtothrips perseae species-group (Thysanoptera), with one new species from avocado, Persea americana.

    Science.gov (United States)

    Mound, Laurence A; Hoddle, Mark S

    2016-02-12

    Following recent molecular studies on avocado thrips, a new species is described from Costa Rica, Ecuador, and Colombia from the young leaves of avocado, Persea americana. Scirtothrips hansoni sp.n. is closely related to the Californian pest, S. perseae, and also to S. astrictus from Costa Rica that remains known from a single female. An illustrated key to these three species is provided.

  5. Susceptibilidad a insectos en selecciones y variedades de annona muricata l. y persea americana m. en Puerto Rico

    Directory of Open Access Journals (Sweden)

    Irma Cabrera

    2001-01-01

    Full Text Available En Puerto Rico se han reportado varios insectos en las guanábanas (Annona muricata L. y los aguacates (Persea americana M, sin embargo los de mayor importancia son el barrenador de la semilla Bephratelloides cubensis (A. y Apate monacha (F. en A. muricata y Pseudacysta persea (F. en P. americana. El objetivo principal de esta investigación fue determinar la incidencia de B. cubensis y A. monacha en seis selecciones de guanábana y en siete variedades de aguacate. De las evaluaciones realizadas en A. muricata, se observó que las selecciones IV-10, VII-14 y VI-1 mostraron la mayor incidencia y la IV-16 mostró la menor incidencia de B. cubensis en relación al número de barrenos por fruta. En cuanto a la incidencia de A. monacha en A. muricata las selecciones IV-16, IV-2, IV-3, VII-14 presentaron la mayor incidencia de barrenos por árbol. En las evaluaciones realizadas en Persea americana se seleccionaron las variedades Ávila, Semil 34, Wilson Popenoe, Pollock, Candelaria, Semil 43 y Butler como las variedades comerciales más sembradas en Puerto Rico. Se observó que la variedad Avila obtuvo el mayor número de huevos, ninfas, hembras y machos de P. persea por hojas comparadas con las demás variedades. Respecto al número de hojas infestadas por árbol se encontró valores significativos (P≤0,01 en la variedad Avila. Resultando tener un 90% de las hojas dañadas. No se observó que las variedades evaluadas tuviese baja o ninguna preferencia a P. Persea

  6. VERIFICACIÓN DEL CONTENIDO DE MATERIA SECA COMO INDICADOR DE COSECHA PARA AGUACATE (persea americana CULTIVAR HASS EN ZONA INTERMEDIA DE PRODUCCIÓN DE LOS SANTOS, COSTA RICA

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    María del Milagro Cerdas Araya

    2014-01-01

    Full Text Available El objetivo de esta investigación fue estu - diar si el contenido de materia seca (CMS de aguacate podría ser utilizado como un índice de cosecha adecuado en sustitución del contenido de aceite (CA en aguacate Hass, cultivado en una zona de altitud intermedia (1750 msnm, en Los Santos, Costa Rica. Se marcaron 100 árboles y un total de 2000 flores polinizadas; se evaluaron 35 frutos cada semana, de la semana 31 a la 44. Se encontró que ambos, CA y CMS, mostraron un aumento rápido y significativo después de la semana 39 (p≥0,05, con un coeficiente de correlación entre ellos de 0,92. Otros cambios observados simultáneamente, y relacionados con la maduración de la fruta, fueron el desarrollo del color morado en la cáscara (74% de la superficie, la reducción de la adherencia de la cubierta de la semilla a la pulpa de la fruta, y de ésta a la cáscara y a la semilla, así como la disminución de la firmeza del fruto, de 118 N durante la semana 31 hasta 57 N en la semana 40 y después de ésta. Se recomienda usar el 23% de CMS en el fruto de aguacate Hass como un índice de cosecha para el aguacate cultivado a 1750 msnm en la zona de Los Santos, como una alternativa al conteni - do de aceite de la fruta (CA.

  7. Assimilate partitioning in avocado, Persea americana

    Energy Technology Data Exchange (ETDEWEB)

    Finazzo, S.; Davenport, T.L.

    1986-04-01

    Assimilate partitioning is being studied in avocado, Persea americana cv. Millborrow in relation to fruit set. Single leaves on girdled branches of 10 year old trees were radiolabeled for 1 hr with 13..mu..Ci of /sup 14/CO/sub 2/. The source leaves were sampled during the experiment to measure translocation rates. At harvest the sink tissues were dissected and the incorporated radioactivity was measured. The translocation of /sup 14/C-labelled compounds to other leaves was minimal. Incorporation of label into fruitlets varied with the tissue and the stage of development. Sink (fruitlets) nearest to the labelled leaf and sharing the same phyllotaxy incorporated the most /sup 14/C. Source leaves for single non-abscising fruitlets retained 3X more /sup 14/C-labelled compounds than did source leaves for 2 or more fruitlets at 31 hrs. post-labelling. Export of label decreased appreciably when fruitlets abscised. If fruitlets abscised within 4 days of labeling then the translocation pattern was similar to the pattern for single fruitlets. If the fruitlet abscised later, the translocation pattern was intermediate between the single and double fruitlet pattern.

  8. Assimilate partitioning in avocado, Persea americana

    International Nuclear Information System (INIS)

    Finazzo, S.; Davenport, T.L.

    1986-01-01

    Assimilate partitioning is being studied in avocado, Persea americana cv. Millborrow in relation to fruit set. Single leaves on girdled branches of 10 year old trees were radiolabeled for 1 hr with 13μCi of 14 CO 2 . The source leaves were sampled during the experiment to measure translocation rates. At harvest the sink tissues were dissected and the incorporated radioactivity was measured. The translocation of 14 C-labelled compounds to other leaves was minimal. Incorporation of label into fruitlets varied with the tissue and the stage of development. Sink (fruitlets) nearest to the labelled leaf and sharing the same phyllotaxy incorporated the most 14 C. Source leaves for single non-abscising fruitlets retained 3X more 14 C-labelled compounds than did source leaves for 2 or more fruitlets at 31 hrs. post-labelling. Export of label decreased appreciably when fruitlets abscised. If fruitlets abscised within 4 days of labeling then the translocation pattern was similar to the pattern for single fruitlets. If the fruitlet abscised later, the translocation pattern was intermediate between the single and double fruitlet pattern

  9. Aislamiento, identificación y evaluación de hongos entomopatógenos como posibles agentes de control de trips (Thysanoptera: Thripidae) asociados a cultivos de aguacate (Persea americana Miller)

    OpenAIRE

    Restrepo Quiróz, Tatiana Inés

    2014-01-01

    Resumen: Los problemas fitosanitarios del sistema productivo del aguacate, como el ataque de insectos plaga, particularmente los trips, afectan la competitividad de este cultivo considerado como una de las apuestas exportadoras del país. Esto hace indispensable la búsqueda de soluciones que respondan al reto de la competitividad y exigencias del mercado. Los trips son insectos difíciles de controlar debido su carácter polífago, su alta tasa reproductiva y hábito críptico. La principal medida ...

  10. USE OF SOIL MICROORGANISMS AS A BIOTECHNOLOGICAL STRATEGY TO ENHANCE AVOCADO (Persea americana-PLANT PHOSPHATE UPTAKE AND GROWTH USO DE MICROORGANISMOS DEL SUELO COMO ESTRATEGIA BIOTECNOLÓGICA PARA MEJORAR LA ABSORCIÓN DE FÓSFORO Y EL CRECIMIENTO DE PLANTAS DE AGUACATE (Persea americana

    Directory of Open Access Journals (Sweden)

    Nelson Walter Osorio Vega

    2012-06-01

    Full Text Available Abstract. In recent years the area cultivated with avocado has increased significantly in Colombia and this trend is expected to continue in the coming years. However, most of the soils in the highlands of the Colombian Andean, that have been used to grow avocado, are strongly acidic and nutrient deficient, particularly phosphorus (P. To alleviate these problems, high rates of lime and P fertilizers are frequently applied, in many cases the applied dose are excessive. This increases production costs, produces plant nutrient imbalance in the plant, and increases the risk of polluting surface waters. A biotechnological alternative is the combined use of phosphate solubilizing microorganisms (PSM and arbuscular mycorhizal fungi (AMF. This work describes a series of experiments carried out to isolate, characterize, and evaluate the effectiveness of AMF and PSM in increasing plant growth and P uptake of avocado plants. The results indicate that the mycorrhizal association is an effective alternative that can easily be established in the crops; however, the excessive use of P fertilizer in these soils may limit the benefits of these fungi on avocado plants. Currently, effective PSM of avocado rhizosphere have not been detected. In some cases, these microorganisms are absent in the avocado rhizosphere and in other cases their low capacity to solubilize P discourage their use in the crop.Resumen. En los últimos años el área cultivada con aguacate en Colombia ha crecido notoriamente y se espera que esta tendencia continúe en los próximos años. Sin embargo, muchos de los suelos de la zona andina utilizados para su siembra exhiben fuerte acidez y niveles deficientes de varios nutrientes, particularmente fósforo (P. Para corregir este problema se hacen aplicaciones de cales y fertilizantes fosfóricos solubles, en mucho casos las dosis aplicadas son altas. Esto eleva innecesariamente los costos de producción, crea desbalance nutricional en la planta

  11. Genetic characterisation of avocado ( Persea americana Mill.) in two ...

    African Journals Online (AJOL)

    Avocado (Persea americana Mill.) is a nutritious and economic tree crop with cultivations scattered all over Ghana. Although, there have been some genetic diversity research on this crop in some parts of the world, rather limited research has been done on avocado in Ghana. A study was therefore conducted to assess its ...

  12. Analgesic effect of the aqueous seed extract of Persea Americana ...

    African Journals Online (AJOL)

    Persea americana, Mill (Lauraceae) is one of the medicinal plants used in Nigeria for pain relief. Based on its ethnomedicinal use in pain management, the seed of the plant was extracted with distilled water and screened for analgesic activity. The analgesic screening was done in mice using four models: acetic ...

  13. Efecto de diferentes métodos de extracción sobre el perfil de ácidos grasos en el aceite de aguacate (Persea americana Mill. var. Hass

    Directory of Open Access Journals (Sweden)

    José Alberto Ariza Ortega

    2011-12-01

    Full Text Available El objetivo de este estudio fue evaluar la posible formación de ácidos grasos trans (AGT en el aceite de aguacate, extraído con disolvente (hexano, con y sin calor, y por centrifugación. Para analizar el aceite se emplearon índices químicos, y para la identificación y cuantificación de los ácidos grasos se utilizó Espectroscopía de Infrarrojo con Transformada de Fourier (EITF, en la región media de 400 a 4000 cm-1 y cromatografía de gases. En los resultados del análisis por EITF se demostró que los tres aceites presentaron un pico definido en la región de 968 cm-1 que corresponde a los dobles enlaces trans. Los AGT se cuantificaron < 0,5 g/100 g de aceite, valores menores que el propuesto por The Danish Veterinary and Food Administration para considerar un aceite libre de AGT. De acuerdo con los índices químicos, el método que causó la mínima modificación en la calidad del aceite fue el de extracción por centrifugación.

  14. Efecto de diferentes métodos de extracción sobre el perfil de ácidos grasos en el aceite de aguacate (Persea americana Mill. var. Hass

    Directory of Open Access Journals (Sweden)

    Arturo Martínez Zavala

    2011-01-01

    Full Text Available El objetivo de este estudio fue evaluar la posible formación de ácidos grasos trans (AGT en el aceite de aguacate, extraído con disolvente (hexano, con y sin calor, y por centrifugación. Para analizar el aceite se emplearon índices químicos, y para la identificación y cuantificación de los ácidos grasos se utilizó Espectroscopía de Infrarrojo con Transformada de Fourier (EITF, en la región media de 400 a 4000 cm E-1 y cromatografía de gases. En los resultados del análisis por EITF se demostró que los tres aceites presentaron un pico definido en la región de 968 cm E-1 que corresponde a los dobles enlaces trans. Los AGT se cuantificaron < 0,5 g/100 g de aceite, valores menores que el propuesto por The Danish Veterinary and Food Administration para considerar un aceite libre de AGT. De acuerdo con los índices químicos, el método que causó la mínima modificación en la calidad del aceite fue el de extracción por centrifugación.

  15. GENETIC VARIABILITY WITHIN MEXICAN RACE AVOCADO (Persea americana Mill. GERMPLASM COLLECTIONS DETERMINED BY ISSRs

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    H. Cuiris-Pérez

    2009-01-01

    Full Text Available El presente estudio se efectuó para determinar la existencia de diversidad genética dentro de una colección de germoplasma de aguacate (Persea americana Mill. perteneciente al Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP, Campo Experimental Uruapan (CEFAP-Uruapan. Se investigó la relación parental entre 77 accesiones (231 plantas de la raza Mexicana con el uso de siete ISSRs (Inter Simple Sequence Repeat microsatellites, en inglés. En total se detectaron 154 loci. El porcentaje de polimorfismo varió de 82.3 a 95.4, con un número de bandas entre 17 y 25 dentro de las accesiones. El análisis de similitud genética reveló la formación de dos grupos principales, uno con once subgrupos y el otro con tres subgrupos. La similitud genética fue más alta entre las accesiones 237 (Atlixco, Puebla y XTC01 (Uruapan, Michoacán, mientras que las accesiones 532 (Atlixco, Puebla y 369 (Chilchota, Michoacán fueron las más disímiles. No se encontraron duplicados en los genotipos analizados. En general, el presente estudio demostró la utilidad del análisis mediante ISSRs para la determinación de diversidad genética en aguacate.

  16. A new species of Tamarixia Mercet (Hymenoptera, Eulophidae, parasitoid of Trioza aguacate Hollis & Martin (Hemiptera, Triozidae in Mexico

    Directory of Open Access Journals (Sweden)

    Zoya Yefremova

    2014-01-01

    Full Text Available Tamarixia aguacatensis Yefremova, sp. n. (Hymenoptera: Eulophidae: Tetrastichinae is described from Mexico as a parasitoid of the avocado psyllid, Trioza aguacate Hollis & Martin (Hemiptera: Triozidae. Trioza aguacate is a serious pest of avocado, Persea americana Miller. A key to the species of Tamarixia Mercet in Mexico is given.

  17. El Copturomímus Perseae Hustache Nueva Especie Entomológica, Grave Plaga del Aguacate en Colombia.

    Directory of Open Access Journals (Sweden)

    Mario Moreno Ernesto

    1947-06-01

    Full Text Available 1. Colombia, por la diversidad de climas y condiciones variadas de suelos, está capacitada para producir ventajosamente las especies frutales que en general son consideradas como de carácter comercial. Entre las cuales, el aguacate, dentro de la zona tropical y subtropical, ocupa lugar prominente, junto con el popular grupo de los Citrus. Aunque la explotación de estas especies frutales se encuentra en forma incipiente, y para mejor decirlo en estado semisilvestre, la correcta vegetación y fructificación abundante lograda dentro de vastas zonas, son un elocuente índice, que unido a la demanda creciente de fruta en los mercados, insatisfecha con la escasa producción nacional, aseguran el éxito de las empresas que consagren sus esfuerzos a la lucrativa industria de la producción de frutas. 2. Ante las perspectivas tan halagadoras que presenta el cultivo del aguacate en el país, y en el convencimiento de que el control de las plagas y enfermedades constituye uno de los factores de mayor importancia, es evidente la necesidad de propender por el establecimiento de las medidas sanitarias, que a la vez que sean económicas, tengan la suficiente efectividad para eliminar el peligro que puedan constituir. 3. El aguacate, dentro de la clasificación botánica pertenece a la familia Lauracea, género Persea y comprende 2 especies principales, Persea americana Mill. (P. gratissimaGaertn y PerseadrymifoliaCham. &Schelecnt. (P.americanavar. DrymifoliaMez. Comercialmente se ha dividido en 3 razas claramente diferenciadas, la Antillana, Guatemalteca y Mexicana, dentro de las cuales se hallan agrupadas variedades en crecido número; aunque existen ciertas variedades que participan de características comunes a dos de las razas mencionadas y se consideran como híbridos o cruces genéticos. Comúnmente tiene diferentes denominaciones que varían de país a país y aun en regiones del mismo país, entre las cuales las más conocidas son, aguacate

  18. The Complex of Scolytinae Inhabiting Persea borbonia and Persea americana in Florida: Possible Interactions with Other Species

    Science.gov (United States)

    A diverse number of Scolytinae (Coleoptera: Curculionidae) have been found inhabiting Persea borbonia and P. americana in Florida during 2009 and 2010. They include the exotic redbay ambrosia beetle, Xyleborus glabratus, vector of Raffaelea lauricola, X. volvulus, X. ferrugineus, Xylosandrus crassiu...

  19. Avocado (Persea americana) seed as a source of bioactive phytochemicals.

    Science.gov (United States)

    Dabas, Deepti; Shegog, Rachel M; Ziegler, Gregory R; Lambert, Joshua D

    2013-01-01

    The pulp of avocado (Persea americana, Lauraceae) has been reported to have beneficial cardiovascular health effects. Avocado oil is used for dermatological applications and its unsaponifiable portion is reported to have beneficial effects against osteoarthritis. Although the seed represents a considerable percentage of the total fruit, scientific research on the phytochemistry and biological effects of avocado seeds is in the nascent stages,. Currently, the seed represents an under-utilized resource and a waste issue for avocado processors. There is ethno-pharmacological information on the use of seeds for the treatment of health-related conditions, especially in South American countries where avocados are endemic and currently grown on a large scale. Current research has shown that avocado seeds may improve hypercholesterolemia, and be useful in the treatment of hypertension, inflammatory conditions and diabetes. Seeds have also been found to possess insecticidal, fungicidal, and anti-microbial activities. The avocado seeds and rich in phenolic compounds, and these may play a role in the putative health effects. Historically, extracts of avocado seeds were also used as ink for writing and research in our laboratory has explored the potential colorant properties of a polyphenol oxidase-produced colored avocado seed extract. Here, we review the currently-available data on the bioactivity and other functional properties of avocado seeds. We discuss the strength of the available data, the putative active compounds, and potential directions for future studies.

  20. Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americana Mill., c.v. Hass

    Directory of Open Access Journals (Sweden)

    Eduardo Padilla-Camberos

    2013-01-01

    Full Text Available The use of vegetal extracts requires toxicological and genotoxic evaluations to establish and verify safety before being added to human cosmetic, pharmaceutical medicine, or alimentary products. Persea americana seeds have been used in traditional medicine as treatment for several diseases. In this work, the ethanolic seed extract of Persea americana was evaluated with respect to its genotoxic potential through micronucleus assay in rodents. The frequency of micronuclei in groups of animals treated with avocado seed extract showed no differences compared to the negative control (vehicle; therefore, it is considered that the avocado seed extract showed no genotoxic activity in the micronucleus test.

  1. Acute toxicity and genotoxic activity of avocado seed extract (Persea americana Mill., c.v. Hass).

    Science.gov (United States)

    Padilla-Camberos, Eduardo; Martínez-Velázquez, Moisés; Flores-Fernández, José Miguel; Villanueva-Rodríguez, Socorro

    2013-01-01

    The use of vegetal extracts requires toxicological and genotoxic evaluations to establish and verify safety before being added to human cosmetic, pharmaceutical medicine, or alimentary products. Persea americana seeds have been used in traditional medicine as treatment for several diseases. In this work, the ethanolic seed extract of Persea americana was evaluated with respect to its genotoxic potential through micronucleus assay in rodents. The frequency of micronuclei in groups of animals treated with avocado seed extract showed no differences compared to the negative control (vehicle); therefore, it is considered that the avocado seed extract showed no genotoxic activity in the micronucleus test.

  2. Description of a new coccid (Hemiptera, Coccidae on avocado (Persea americana Mill. from Colombia, South America

    Directory of Open Access Journals (Sweden)

    Takumasa Kondo

    2010-04-01

    Full Text Available A new soft scale insect, Bombacoccus aguacatae Kondo, gen. n. and sp. n. (Hemiptera: Coccidae collected on the branches and twigs of avocado, Persea americana Mill. (Lauraceae in Colombia, is described and illustrated based on the adult female. An updated taxonomic key to closely related genera of the Toumeyella-group is provided.

  3. Race, Interracial Admixture and Genetic Differentiation of Avocado (Persea americana Mill)

    Science.gov (United States)

    Avocado (Persea americana Mill.) is a major tropical fruit crop native to Mesoamerica and domesticated around 8000 to 7000 BC. It is a member of the Laureace family and currently classified into three subspecies or races: Guatemalan (G), Mexican (M) and West Indian (WI) according to their ecological...

  4. CARACTERIZACIÓN Y ACTIVIDAD ANTIOXIDANTE DEL ACEITE DE LA SEMILLA DE PALTA Persea americana MILL.

    OpenAIRE

    Rengifo, Pedro G.; Carhuapoma, Mario; Artica, Luis; Castro, Américo J.; López, Sofía

    2017-01-01

    La semilla de palta es una parte del fruto descartada en la obtención industrial del aceite de pulpa y podría ser una fuente de ácidos grasos esenciales y otros fitoconstituyentes. El presente trabajo de investigación tuvo como objetivo caracterizar al aceite de la semilla de Persea americana Mill. Var. Hass Fuerte y su actividad antioxidante. Al aceite obtenido por el método de Soxhlet, se le hizo la caracterización fisicoquímica siguiendo las normas AOCS (Sociedad Americana de la Química de...

  5. Pistil Starch Reserves at Anthesis Correlate with Final Flower Fate in Avocado (Persea americana)

    OpenAIRE

    Alcaraz, Mar?a Librada; Hormaza, Jos? Ignacio; Rodrigo, Javier

    2013-01-01

    A common observation in different plant species is a massive abscission of flowers and fruitlets even after adequate pollination, but little is known as to the reason for this drop. Previous research has shown the importance of nutritive reserves accumulated in the flower on fertilization success and initial fruit development but direct evidence has been elusive. Avocado (Persea americana) is an extreme case of a species with a very low fruit to flower ratio. In this work, the implications of...

  6. Antidiabetic potential of methanol extracts from leaves of Piper umbellatum L. and Persea americana Mill.

    Directory of Open Access Journals (Sweden)

    Guy Sedar Singor Njateng

    2018-01-01

    Full Text Available Objective: To determine inhibitory activity of methanolic leaf extract of Piper umbellatum and Persea americana (P. americana (traditionally used in Cameroon against diabetes on α -glucosidase, β -glucosidase, maltase-glucoamylase, aldose reductase and aldehyde reductase activities, enzymes involved in starch digestion or diabetic complications. Methods: The methanol extracts from Piper umbellatum and P. americana were prepared by maceration. To assess relative efficacy of these extracts, the determination of concentrations that were needed to inhibit 50% of enzyme activity was done, whereas, gas chromatography-mass spectrum was used to identify components from extracts that may be responsible for the activities. Results: The tested extracts strongly inhibited α -glucosidase, maltase-glucoamylase, aldose reductase and aldehyde reductase activities with IC50 ranging from (1.07 ± 0.03 to (31.77 ± 1.17 μg/mL. Among the tested extracts, P. americana was the most active against sensitive enzymes (IC50 of 1.07 ± 0.03 to 15.63 ± 1.23. But, none of the extracts showed interesting inhibitory effect against β -glucosidase as their percentage inhibitions were less than 16%. From gas chromatography-mass spectrum analysis, 10 and 8 compounds were identified in Piper umbellatum and P. americana extracts respectively, using NIST library 2014. Conclusions: Results of this study provide the scientific credential for a prospective usage of these plants to treat diabetes.

  7. Preliminar toxicological assesement of Ruta graveolens, Origanum vulgare and Persea americana on the preimplantational mouse embryos

    Directory of Open Access Journals (Sweden)

    V. Benavides

    2014-06-01

    Full Text Available The growing interest in natural medicine makes it necessary to study plant properties as well as their possible secondary effects. In recent years the toxic effects of many medicinal plants on the preimplantational mouse embryo development have been studied. Many of them produce malformations and alterations in the embryonic development. Ruta graveolens "ruda", Origanum vulgare "oregano" and Persea americana "palta" are used in rural areas to menstrual colic and to provoke abortion (estrella, 1995. This study is aimed at assessing "in vivd'the effect of extracts of "oregano", "ruda" and "palta" to 20% on the morphology and growth of preimplantational mouse embryos.

  8. Hongos Fitopatógenos asociados a semillas de palto (Persea Americana mill.)

    OpenAIRE

    Leon Ttacca, Betsabe; Mattos Calderon, Leonor

    2016-01-01

    Una serie de enfermedades severas de origen fungoso son diseminadas por semillas de palto, y pueden causar una disminución de la producción en aproximadamente el 14 % y una reducción de calidad en un 10 %. El presente trabajo consistió en aislar e identificar hongos fitopatógenos presentes en las semillas de palto (Persea americana Mill.) raza mexicana utilizadas para patrón. A partir del embrión y cotiledón de 200 semillas de palto, se aislo los hongos fotopatogenos en medio de cultivo Papa ...

  9. Effects of ripening on rheological properties of avocado pulp (Persea americana mill. Cv. Hass)

    Science.gov (United States)

    Osorio, F.; Roman, A.; Ortiz, J.

    2015-04-01

    Avocado (Persea americana Mill) Hass variety is the most planted in Chile with a greater trade prospect. The aim of this study was to investigate the effect of maturity on rheological properties of Chilean Avocado Hass pulp. Fresh unripe avocados were washed and peeled, cut and stored at 3 different times; a portion was treated at 5°C and the other was treated at 20°C until it reached 2 lb puncture pressure. During maturation changes would develop due to temperature and time, with internal cellular structure changes. Preliminary results of the rheological characteristics of avocado puree show a Bingham plastic behavior.

  10. Isolasi Senyawa Aktif Ekstrak Etanol Biji Alpukat (Persea americana dan Uji Toksisitas Terhadap Artemia Salina Leach

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    Andi Nur Fitriani Abubakar

    2014-07-01

    Full Text Available Avocado seed (Persea americana is recognized as one of medicinal plants. It contains several secondary metabolites, which have toxic activity. However, efforts to identify active compounds from avocado seeds (Persea americana are still relatively rare. Therefore, isolation and toxicity assay have been conducted foward the active compound of avocado seed. Maceration one kilogram of seed dried powder by ethanol obtained 49,7464 gram extract. Separation of etanol extract by column chromatography generated 0,0698 grams of pure white needle crytal, which is positively triterpenoid based on Lieberman-Buchard test. In addition, infrared spectrum showed the existence of OH, C=C, C-C, C=O, -C-H, -CH3,-CH2 and C-O stretch, which support the presumed compound. The result of toxicity test on Artemia salina Leach showed that the extract, fraction and pure isolates of the etanol extract are toxic with LC50 values 13,274 g/mL; 9,528 g/mL and 8,128 g/mL, respectively.

  11. Transcriptional signatures of ancient floral developmental genetics in avocado (Persea americana; Lauraceae).

    Science.gov (United States)

    Chanderbali, André S; Albert, Victor A; Leebens-Mack, Jim; Altman, Naomi S; Soltis, Douglas E; Soltis, Pamela S

    2009-06-02

    The debate on the origin and evolution of flowers has recently entered the field of developmental genetics, with focus on the design of the ancestral floral regulatory program. Flowers can differ dramatically among angiosperm lineages, but in general, male and female reproductive organs surrounded by a sterile perianth of sepals and petals constitute the basic floral structure. However, the basal angiosperm lineages exhibit spectacular diversity in the number, arrangement, and structure of floral organs, whereas the evolutionarily derived monocot and eudicot lineages share a far more uniform floral ground plan. Here we show that broadly overlapping transcriptional programs characterize the floral transcriptome of the basal angiosperm Persea americana (avocado), whereas floral gene expression domains are considerably more organ specific in the model eudicot Arabidopsis thaliana. Our findings therefore support the "fading borders" model for organ identity determination in basal angiosperm flowers and extend it from the action of regulatory genes to downstream transcriptional programs. Furthermore, the declining expression of components of the staminal transcriptome in central and peripheral regions of Persea flowers concurs with elements of a previous hypothesis for developmental regulation in a gymnosperm "floral progenitor." Accordingly, in contrast to the canalized organ-specific regulatory apparatus of Arabidopsis, floral development may have been originally regulated by overlapping transcriptional cascades with fading gradients of influence from focal to bordering organs.

  12. Antioxidant activity of hydroalcoholic leaf extracts of Psidium guajava and Persea Americana, an invitro study

    International Nuclear Information System (INIS)

    Kumar, Amith; Kumarachandra, Reshma; Rai, Rajalakshmi; Rao, Satish; Suchetha Kumari; Joshi, Manjunath

    2013-01-01

    Ionizing radiation produce deleterious effects in the living organisms. Rapid technological advancement has increased human exposure to ionizing radiations enormously. Several plants have been screened for their radio protective ability and the hunt for identifying many more safe, nontoxic and effective ones is in progress. The aim of the present study was to evaluate the antioxidant activity in hydro alcoholic leaf extract of Psidium guajava (guava) and Persea americana (avocado). The antioxidant assay such as 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) free radical scavenging assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, chelation of iron by plant extract, hydroxyl radical scavenging assay, ferric reducing antioxidant potential (FRAP), and total antioxidant activity of extract was analyzed using spectrophotometer. Phytochemical screening and HPLC analysis of leaf extracts were also performed. The results indicated that the IC50 value of hydro alcoholic leaf extract of P.guajava and P. americana were 14 μg/ml and 15 μg/ml in ABTS free radical scavenging assay, 4 μg/ml and 2.5 μg/ml in DPPH free radical scavenging assay, 7.2 μg/ml and 8.4 μg/ml in chelation of iron by plant extract, 2 μg/ml and, 4.3 μg/ml in hydroxyl radical scavenging assay and effective concentration(EC50) was 57.80 μg/ml and 87.56 μg/ml in FRAP assay respectively. The results for total antioxidant activity indicated that 242.3 μg/ml of P.guajava and 432.7 μg/ml of P.americana extract was equivalent to 100 μg/ml of standard qurecetin respectively. Qualitative analysis of hydroalcoholic leaf extracts revealed the presence of flavonoids, tannins, saponions, terpenoids, and glycosides. HPLC analysis confirmed the presence of flavonoids qurecetin and rutin in leaf extracts on comparison with standard compounds. Our study showed that hydro alcoholic leaf extracts of Psidium guajava and Persea americana act as strong antioxidant and free

  13. Quality of fresh-cut avocado (Persea americana Mill. stored under different temperatures Qualidade de abacates (Persea americana Mill. minimally processados armazenados sob diferentes temperaturas

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    Ana Carla Marques Pinheiro

    2009-08-01

    Full Text Available The goal of this work was to evaluate the effect of different storage temperatures on the quality maintenance of fresh-cut 'Fortuna' avocado (Persea americana Mill.. The fruit was selected, washed and sanitized with sodium hypochlorite solution (190 ppm total residual chlorinee for 15 minutes. After that, the tip was cut and the fruit was also cut in halves, peeled and the pit removed, manually. Then the halves were immersed in 0.5% calcium chlorite + 0.5% cysteine solution for 2 minutes, to avoid darkening and packed in rigid plastic covered with 20 micrometer thick plastic wrap (PVC film with a permeability to O2, CO2 and water vapor of 15,000, 80,000 and 390 cm³/m²/day, respectively. The product was stored under 3 different temperatures 0, 5, 10ºC and evaluated at 5 different storege periods of 0, 2, 3, 5 and 6 days, in a randomized design in a 3x5 factorial. The storage of fresh-cut avocado at 5ºC is the best alternative because it determines better maintenance of quality of the avocado. These presented lower darkening of pulp, better scores for appearance and acceptability, lower softening and polyphenoloxidase activity, in comparison to those stored at 0 and 10ºC. The fresh-cut avocados stored at 5ºC presented a shelf life of 5 days, based, mainly on, appearance, score 7.3, sited between the concepts "like moderately" and "like very much" on a hedonic scale of 9 points and 86% of acceptability, at the end of 5 days of storage.Objetivou-se avaliar o efeito de diferentes temperaturas de armazenamento na manutenção da qualidade de abacates 'Fortuna' (Persea americana Mill. minimally processados. Os frutos foram lavados e sanificados em solução de hipoclorito de sódio (190 ppm de cloro residual total por 15 minutos. Posteriormente, efetuou-se a retirada da extremidade onde se insere o pedúnculo, o corte em metades, retirada do caroço e casca, manualmente. Em seguida, as metades foram imersas em solução com cloreto de cálcio 0

  14. Pro-apoptotic effect of Persea americana var. Hass (avocado) on Jurkat lymphoblastic leukemia cells.

    Science.gov (United States)

    Bonilla-Porras, Angelica R; Salazar-Ospina, Andrea; Jimenez-Del-Rio, Marlene; Pereañez-Jimenez, Andres; Velez-Pardo, Carlos

    2013-11-05

    Abstract Context: Therapy for leukemia has a limited efficacy. There is a need to search for alternative anti-leukemia therapies. Persea americana Mill var. Hass (Lauraceae) is a tropical fruit (avocado) that might be used against cancer. Objective: To investigate whether P. americana induces death in Jurkat lymphoblastic leukemia cells. Materials and methods: Four ethanol extracts (0.1, 0.5, 1, 2 and 5 mg/mL) from avocado fruit (endocarp, whole seed, seed and leaves) were analyzed against Jurkat cells. Hydrogen peroxide generation by oxidation of 2',7'-dichlorodihydrofluorescein diacetate to the fluorescent compound 2',7'-dichlorfluorescein assay, acridine orange/ethidium bromide staining, flow cytometry analysis of annexin-V/7-amino-actinomycin, mitochondrial membrane potential and immunocytochemistry detection of transcription factor p53, caspase-3 and apoptosis-inducing factor (AIF) were evaluated. Results: Endocarp, seed, whole seed, and leaf (0.1 mg/mL) extracts induced significant apoptosis in Jurkat cells (p avocado and its therapeutic action on leukemia.

  15. Studies on the Paint Forming Properties of Avocado(Persea Americana) and African Pear (Dacryodes Edulis) Seed Oils.

    OpenAIRE

    Otaigbe, J.O.E; Oriji, O.G; Ekerenam, G.E

    2016-01-01

    Avocado(Persea Americana) and African Pear (Dacryodes edulis) seed oils were investigated for their suitability as base materials for oil paint production. Soxhlet extraction of the oils from the powdered seeds using n-hexane gave 3.63% and 10.40% yields for Avocado and African Pear respectively. Proximate analysis and chemical characterization of the seed oils were carried out using standard procedures according to the American Oil Chemist Society (AOCS) and the American Society for Testing ...

  16. Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars.

    Science.gov (United States)

    Raymond Chia, Teck Wah; Dykes, Gary A

    2010-07-01

    The epicarp and seed of Persea Americana Mill. var. Hass (Lauraceae), Persea Americana Mill. var. Shepard, and Persea americana Mill. var Fuerte cultivars of mature avocados (n = 3) were ground separately and extracted with both absolute ethanol and distilled water. Extracts were analyzed for antimicrobial activity using the microtiter broth microdilution assay against four Gram-positive bacteria, six Gram-negative bacteria, and one yeast. Antimicrobial activity against two molds was determined by the hole plate method. The ethanol extracts showed antimicrobial activity (104.2-416.7 microg/mL) toward both Gram-positive and Gram-negative bacteria (except Escherichia coli), while inhibition of the water extracts was only observed for Listeria monocytogenes (93.8-375.0 microg/mL) and Staphylococcus epidermidis (354.2 microg/mL). The minimum concentration required to inhibit Zygosaccharomyces bailii was 500 microg/mL for the ethanol extracts, while no inhibition was observed for the water extracts. No inhibition by either ethanol or water extracts was observed against Penicillium spp. and Aspergillus flavus.

  17. Utilization of modified starch from avocado (Persea americana Mill.) seed in cream soup production

    Science.gov (United States)

    Cornelia, M.; Christianti, A.

    2018-01-01

    Avocado (Persea americana Mill.) seed was often seen as waste and underutilized resources, especially in the food industry. The aim of this research was to modify the structure of avocado seed starch using the cross-linking method, to improve the viscosity stability in the cream soup. In the preliminary research, starch was isolated from the seed and modified by STPP (sodium tripolyphosphate) with 2%, 4%, and 6% concentration and were reacted for 1, 2, and 3 hours. Starches were analyzed for moisture and ash content, paste clarity, gel strength, swelling power, solubility, yield, and degree of whiteness. Based on the analysis results, the best reaction time and STPP concentration was 6% at 1 hour reaction time. Native starch and the best-modified starch were applied in the cream soup and compared with commercial cream soup. Cream soups were analyzed for viscosity stability using viscometer in 0, 1, 3, and 5 hours after storage in room temperature. The result showed that cream soup using modified starch has better viscosity stability than native starch and commercial cream soup after 5 hours storage, which was 181.7 ± 4.85 cP. Sensory analysis showed that cream soup using modified starch was more acceptable than the others. Avocado seed modified starch has phosphate group that strengthen the starch chain to prevent viscosity breakdown.

  18. Effects of Persea americana Mill. seed extracts on the postembryonic development of Musca domestica (Diptera: Muscoide

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    Silvia del C. Molina Bertrán

    2018-04-01

    Full Text Available Context: The synthetic insecticides used to control Diptera are harmful to the environment and humans. Extracts and compounds from plants are a more sustainable source for the development of bio-insecticides. Aims: To evaluate the efficacy of a hydroalcoholic extract of Persea americana Mill seeds as an alternative control of the species Musca domestica. Methods: The extracts were obtained by two methods, the Shaker (S and the Soxhlet extraction (SE method, using 94% ethanol as the solvent. Also, the qualitative chemical composition was determined by phytochemical screening. The effect of the two extracts on the post-embryonic development of the fly as well as the adulticidal effect was evaluated. Results: Phytochemical analysis revealed the presence of metabolites such as alkaloids, coumarins, tannins, flavonoids, sugars and amino acids. The influence on the post-embryonic development of M. domestica was demonstrated, especially on the viability of larvae and neolarvae to adults; however, the effect on the weight and duration of each period was low. The adulticidal effects of the extracts were determined by the lethal concentration 50(LC50 of 2.910 mg/100 mL and 3.944 mg/100 mL for the S and SE extracts, respectively. Conclusions: Both extracts showed their insecticidal effects against Musca domestica, but the extract elaborated by S method showed greater influence diminishing viability and better adulticidal effect.

  19. Microsatellite markers in avocado (Persea americana Mill.): genealogical relationships among cultivated avocado genotypes.

    Science.gov (United States)

    Ashworth, V E T M; Clegg, M T

    2003-01-01

    Twenty-five microsatellite markers uniquely differentiated 35 avocado cultivars and two wild relatives. Average heterozygosity was high (60.7%), ranging from 32% in P. steyermarkii to 84% in Fuerte and Bacon. In a subset of 15 cultivars, heterozygosity averaged 63.5% for microsatellites, compared to 41.8% for restriction fragment length polymorphisms (RFLPs). A neighbor-joining tree, according to average shared allele distances, consisted of three clusters likely corresponding to the botanical races of avocado and intermediate clusters uniting genotypes of presumably racially hybrid origin. Several results were at odds with existing botanical assignments that are sometimes rendered difficult by incomplete pedigree information, the complexity of the hybrid status (multiple backcrossing), or both. For example, cv. Harvest clustered with the Guatemalan race cultivars, yet it is derived from the Guatemalan x Mexican hybrid cv. Gwen. Persea schiedeana grouped with cv. Bacon. The rootstock G875 emerged as the most divergent genotype in our data set. Considerable diversity was found particularly among accessions from Guatemala, including G810 (West Indian race), G6 (Mexican race), G755A (hybrid Guatemalan x P. schiedeana), and G875 (probably not P. americana). Low bootstrap support, even upon exclusion of (known) hybrid genotypes from the data matrix, suggests the existence of ancient hybridization or that the botanical races originated more recently than previously thought.

  20. Anti Lithiasis Activity of Avocado (Persea americana Mill Leaves Extract in White Male Rats

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    IETJE WIENTARSIH

    2012-03-01

    Full Text Available In Indonesia, avocado leaves have been used as traditional medicines for diureticum to cure urolithiasis. This research was to determine anti lithiasis activity of avocado leaves (Persea americana Mill extract on white male rats nefrolithiasis model induced by ethylene glycol. Ethanol extraction method was used to get extract of avogadro leaves. Twenty adult male white rats were divided into 4 different induction treatments i.e. aquadest, ethylene glycol 0.75% and ammonium chloride 2%, and extract of avocado leaves with different levels of 100 and 300 mg/kg bw respectively. Their body weight was measured daily to determine their growth ratio. And at the end of the trial, the kidney was analyzed its calcium level and inhibitory activity to formation of calcium oxalate crystals. The results showed that the amount of calcium level in the kidney of rats treated with extract of avogadro leaves was significantly decreased than that of rats treated with ethylene glycol 0.75% and ammonium chloride 2% (P < 0.05. The extract avocado leaves as a herbal remedy can be recommended as a phytotherapeutic agent especially for preventive action for urolithiasis diseases.

  1. Purification and characterization of peroxidase from avocado (Persea americana Mill, cv. Hass).

    Science.gov (United States)

    Rojas-Reyes, José O; Robles-Olvera, Victor; Carvajal-Zarrabal, Octavio; Castro Matinez, Claudia; Waliszewski, Krzysztof N; Aguilar-Uscanga, María Guadalupe

    2014-07-01

    Avocado (Persea americana Mill, cv. Hass) fruit ranks tenth in terms of the most important products for Mexico. Avocado products are quite unstable due to the presence of oxidative enzymes such as polyphenol oxidase and peroxidase. The present study is to characterize the activity of purified avocado peroxidase from avocado in order to ascertain the biochemical and kinetic properties and their inhibition conditions. Purification was performed by Sephacryl S 200 HR gel filtration chromatography and its estimated molecular weight was 40 kDa. The zymogram showed an isoelectric point of 4.7. Six substrates were tested in order to ascertain the affinity of the enzyme for these substrates. The purified peroxidase was found to have low Km (0.296 mM) and high catalytic efficiency (2688 mM(-1) s(-1)) using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), optimum activity being reached at 51°C, pH 3.8. The addition of dithiothreitol, β-mercaptoethanol, ascorbic acid, sodium azide, L-cysteine and Tween-20 had high inhibitory effects, while metals ions such as Cu(+), Fe(2+) and Mn(2+) had weak inhibitory activity on purified avocado peroxidase. The purified avocado peroxidase exhibits high inhibition (Ki = 0.37 µM) with 1.97 µM n-propyl gallate using ABTS as substrate at 51°C, pH 3.8 for 10 min. © 2013 Society of Chemical Industry.

  2. Transcriptomic Analysis of Avocado Hass (Persea americana Mill) in the Interaction System Fruit-Chitosan-Colletotrichum.

    Science.gov (United States)

    Xoca-Orozco, Luis-Ángel; Cuellar-Torres, Esther Angélica; González-Morales, Sandra; Gutiérrez-Martínez, Porfirio; López-García, Ulises; Herrera-Estrella, Luis; Vega-Arreguín, Julio; Chacón-López, Alejandra

    2017-01-01

    Avocado ( Persea americana ) is one of the most important crops in Mexico as it is the main producer, consumer, and exporter of avocado fruit in the world. However, successful avocado commercialization is often reduced by large postharvest losses due to Colletotrichum sp., the causal agent of anthracnose. Chitosan is known to have a direct antifungal effect and acts also as an elicitor capable of stimulating a defense response in plants. However, there is little information regarding the genes that are either activated or repressed in fruits treated with chitosan. The aim of this study was to identify by RNA-seq the genes differentially regulated by the action of low molecular weight chitosan in the avocado-chitosan- Colletotrichum interaction system. The samples for RNA-seq were obtained from fruits treated with chitosan, fruits inoculated with Colletotrichum and fruits both treated with chitosan and inoculated with the fungus. Non-treated and non-inoculated fruits were also analyzed. Expression profiles showed that in short times, the fruit-chitosan system presented a greater number of differentially expressed genes, compared to the fruit-pathogen system. Gene Ontology analysis of differentially expressed genes showed a large number of metabolic processes regulated by chitosan, including those preventing the spread of Colletotrichum . It was also found that there is a high correlation between the expression of genes in silico and qPCR of several genes involved in different metabolic pathways.

  3. Persea americana (avocado): bringing ancient flowers to fruit in the genomics era.

    Science.gov (United States)

    Chanderbali, André S; Albert, Victor A; Ashworth, Vanessa E T M; Clegg, Michael T; Litz, Richard E; Soltis, Douglas E; Soltis, Pamela S

    2008-04-01

    The avocado (Persea americana) is a major crop commodity worldwide. Moreover, avocado, a paleopolyploid, is an evolutionary "outpost" among flowering plants, representing a basal lineage (the magnoliid clade) near the origin of the flowering plants themselves. Following centuries of selective breeding, avocado germplasm has been characterized at the level of microsatellite and RFLP markers. Nonetheless, little is known beyond these general diversity estimates, and much work remains to be done to develop avocado as a major subtropical-zone crop. Among the goals of avocado improvement are to develop varieties with fruit that will "store" better on the tree, show uniform ripening and have better post-harvest storage. Avocado transcriptome sequencing, genome mapping and partial genomic sequencing will represent a major step toward the goal of sequencing the entire avocado genome, which is expected to aid in improving avocado varieties and production, as well as understanding the evolution of flowers from non-flowering seed plants (gymnosperms). Additionally, continued evolutionary and other comparative studies of flower and fruit development in different avocado strains can be accomplished at the gene expression level, including in comparison with avocado relatives, and these should provide important insights into the genetic regulation of fruit development in basal angiosperms.

  4. Bitter-tasting and kokumi-enhancing molecules in thermally processed avocado (Persea americana Mill.).

    Science.gov (United States)

    Degenhardt, Andreas Georg; Hofmann, Thomas

    2010-12-22

    Sequential application of solvent extraction and RP-HPLC in combination with taste dilution analyses (TDA) and comparative TDA, followed by LC-MS and 1D/2D NMR experiments, led to the discovery of 10 C(17)-C(21) oxylipins with 1,2,4-trihydroxy-, 1-acetoxy-2,4-dihydroxy-, and 1-acetoxy-2-hydroxy-4-oxo motifs, respectively, besides 1-O-stearoyl-glycerol and 1-O-linoleoyl-glycerol as bitter-tasting compounds in thermally processed avocado (Persea americana Mill.). On the basis of quantitative data, dose-over-threshold (DoT) factors, and taste re-engineering experiments, these phytochemicals, among which 1-acetoxy-2-hydroxy-4-oxo-octadeca-12-ene was found with the highest taste impact, were confirmed to be the key contributors to the bitter off-taste developed upon thermal processing of avocado. For the first time, those C(17)-C(21) oxylipins exhibiting a 1-acetoxy-2,4-dihydroxy- and a 1-acetoxy-2-hydroxy-4-oxo motif, respectively, were discovered to induce a mouthfulness (kokumi)-enhancing activity in sub-bitter threshold concentrations.

  5. “efectos de los antioxidantes en el aguacate con la aplicación de cobertura de polímeros naturales (maíz y yuca) durante la postcosecha a temperatura ambiente en pruebas experimentales”

    OpenAIRE

    Acosta Dávila, Sandra; Alvarado Suárez, María José; Sánchez Ávila, Samuel

    2013-01-01

    La necesidad de prolongar el tiempo de almacenamiento postcosecha del aguacate y aliviar el daño ocasionado durante el mismo en condiciones ambientales, nos impulsa a realizar este estudio en la variedad persea americana. Con el desarrollo de esta técnica se espera obtener un aumento del tiempo de vida útil postcosecha, evaluando los resultados fisiológicos y físicos pre establecidos como factores de calidad siendo estos: Sabor por evaluación sensorial, cambios de color del aguacate evalua...

  6. Distinción de especies del género Persea mediante RAPD e ISSR de ADN

    OpenAIRE

    Reyes-Alemán, Juan Carlos; Valadez-Moctezuma, Ernestina; Simuta-Velázco, Lisandro; Barrientos-Priego, Alejandro Facundo; Gallegos-Vázquez, Clemente

    2013-01-01

    Con la finalidad de establecer bases para diferenciar parte de la diversidad genética de Persea y en especial del subgénero Persea resguardado en la colección nacional de germoplasma de aguacate de México, se estudiaron ocho especies (P. americana, P. steyermarkii, P. schiedeana, P. lingue, P. nubigena, P. floccosa, P. cinerascens y P. indica) con marcadores moleculares mediante las técnicas de RAPD e ISSR, donde los productos de PCR fueron separados en geles de acrilamida. Las huellas de ADN...

  7. Competitividad de la oferta exportable de la palta Hass (Persea americana) en el mercado de Estados Unidos

    OpenAIRE

    Balvín Calderón, Eduardo Simeón

    2016-01-01

    Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Agronegocios La presente investigación denominada “Competitividad de la oferta exportable de la palta Hass (Persea americana) en el Mercado de Estados Unidos”, se realizó en el departamento de Lima e Ica, durante el periodo del 2004 al 2014. El propósito de la investigación fue realizar el análisis la oferta exportable, además se analizó las características del mercado de Estados Unidos de América y el nivel de compet...

  8. Terpenoid variations within and among half-sibling avocado trees, Persea americana Mill. (Lauraceae.

    Directory of Open Access Journals (Sweden)

    Jerome Niogret

    Full Text Available Chemical analyses were conducted to determine the qualitative and quantitative differences in monoterpenes and sesquiterpenes in plant material from avocado trees, Persea americana Mill. (Lauraceae. The initial study analyzed plant material sampled from the trunk to the leaves through different branch diameters to quantify proximo-distal spatial differences within a tree. All trees were seedlings initiated from a single maternal tree. Two-way analysis of variance was conducted on 34 chemicals that comprised at least 3% of the total chemical content of at least one tree and/or location within a tree. There were significant interactions between genotype and location sampled for most chemicals. Parentage analysis using microsatellite molecular markers (SSR's determined that the four trees had three fathers and that they represented two full-siblings and two half-sibling trees. Descriptive discriminant analysis found that both genotype and location within a tree could be separated based on chemical content, and that the chemical content from full-siblings tended to be more similar than chemical content from half-siblings. To further explore the relationship between genetic background and chemical content, samples were analyzed from leaf material from 20 trees that included two sets of full-sibling seedling trees, the maternal tree and the surviving paternal tree. Descriptive discriminant analysis found good separation between the two full-sibling groups, and that the separation was associated with chemistry of the parental trees. Six groups of chemicals were identified that explained the variation among the trees. We discuss the results in relation to the discrimination process used by wood-boring insects for site-selection on host trees, for tree selection among potential host trees, and the potential use of terpenoid chemical content in chemotaxonomy of avocado trees.

  9. Pistil starch reserves at anthesis correlate with final flower fate in avocado (Persea americana.

    Directory of Open Access Journals (Sweden)

    María Librada Alcaraz

    Full Text Available A common observation in different plant species is a massive abscission of flowers and fruitlets even after adequate pollination, but little is known as to the reason for this drop. Previous research has shown the importance of nutritive reserves accumulated in the flower on fertilization success and initial fruit development but direct evidence has been elusive. Avocado (Persea americana is an extreme case of a species with a very low fruit to flower ratio. In this work, the implications of starch content in the avocado flower on the subsequent fruit set are explored. Firstly, starch content in individual ovaries was analysed from two populations of flowers with a different fruit set capacity showing that the flowers from the population that resulted in a higher percentage of fruit set contained significantly more starch. Secondly, in a different set of flowers, the style of each flower was excised one day after pollination, once the pollen tubes had reached the base of the style, and individually fixed for starch content analysis under the microscope once the fate of its corresponding ovary (that remained in the tree was known. A high variability in starch content in the style was found among flowers, with some flowers having starch content up to 1,000 times higher than others, and the flowers that successfully developed into fruits presented significantly higher starch content in the style at anthesis than those that abscised. The relationship between starch content in the ovary and the capacity of set of the flower together with the correlation found between the starch content in the style and the fate of the ovary support the hypothesis that the carbohydrate reserves accumulated in the flower at anthesis are related to subsequent abscission or retention of the developing fruit.

  10. Terpenoid variations within and among half-sibling avocado trees, Persea americana Mill. (Lauraceae).

    Science.gov (United States)

    Niogret, Jerome; Epsky, Nancy D; Schnell, Raymond J; Boza, Edward J; Kendra, Paul E; Heath, Robert R

    2013-01-01

    Chemical analyses were conducted to determine the qualitative and quantitative differences in monoterpenes and sesquiterpenes in plant material from avocado trees, Persea americana Mill. (Lauraceae). The initial study analyzed plant material sampled from the trunk to the leaves through different branch diameters to quantify proximo-distal spatial differences within a tree. All trees were seedlings initiated from a single maternal tree. Two-way analysis of variance was conducted on 34 chemicals that comprised at least 3% of the total chemical content of at least one tree and/or location within a tree. There were significant interactions between genotype and location sampled for most chemicals. Parentage analysis using microsatellite molecular markers (SSR's) determined that the four trees had three fathers and that they represented two full-siblings and two half-sibling trees. Descriptive discriminant analysis found that both genotype and location within a tree could be separated based on chemical content, and that the chemical content from full-siblings tended to be more similar than chemical content from half-siblings. To further explore the relationship between genetic background and chemical content, samples were analyzed from leaf material from 20 trees that included two sets of full-sibling seedling trees, the maternal tree and the surviving paternal tree. Descriptive discriminant analysis found good separation between the two full-sibling groups, and that the separation was associated with chemistry of the parental trees. Six groups of chemicals were identified that explained the variation among the trees. We discuss the results in relation to the discrimination process used by wood-boring insects for site-selection on host trees, for tree selection among potential host trees, and the potential use of terpenoid chemical content in chemotaxonomy of avocado trees.

  11. Pistil starch reserves at anthesis correlate with final flower fate in avocado (Persea americana).

    Science.gov (United States)

    Alcaraz, María Librada; Hormaza, José Ignacio; Rodrigo, Javier

    2013-01-01

    A common observation in different plant species is a massive abscission of flowers and fruitlets even after adequate pollination, but little is known as to the reason for this drop. Previous research has shown the importance of nutritive reserves accumulated in the flower on fertilization success and initial fruit development but direct evidence has been elusive. Avocado (Persea americana) is an extreme case of a species with a very low fruit to flower ratio. In this work, the implications of starch content in the avocado flower on the subsequent fruit set are explored. Firstly, starch content in individual ovaries was analysed from two populations of flowers with a different fruit set capacity showing that the flowers from the population that resulted in a higher percentage of fruit set contained significantly more starch. Secondly, in a different set of flowers, the style of each flower was excised one day after pollination, once the pollen tubes had reached the base of the style, and individually fixed for starch content analysis under the microscope once the fate of its corresponding ovary (that remained in the tree) was known. A high variability in starch content in the style was found among flowers, with some flowers having starch content up to 1,000 times higher than others, and the flowers that successfully developed into fruits presented significantly higher starch content in the style at anthesis than those that abscised. The relationship between starch content in the ovary and the capacity of set of the flower together with the correlation found between the starch content in the style and the fate of the ovary support the hypothesis that the carbohydrate reserves accumulated in the flower at anthesis are related to subsequent abscission or retention of the developing fruit.

  12. Avocado fruit (Persea americana Mill) exhibits chemo-protective potentiality against cyclophosphamide induced genotoxicity in human lymphocyte culture.

    Science.gov (United States)

    Paul, Rajkumar; Kulkarni, Paresh; Ganesh, Narayan

    2011-01-01

    Diets rich in fruits and vegetables have been associated with reduced risks for many types of cancers. Avocado (Persea americana Mill.) is a widely consumed fruit containing many cancer preventing nutrients, vitamins and phytochemicals. Studies have shown that phytochemicals extracted from the avocado fruit selectively induce cell cycle arrest, inhibit growth, and induce apoptosis in precancerous and cancer cell lines. Our recent studies indicate that phytochemicals extracted with 50% Methanol from avocado fruits help in proliferation of human lymphocyte cells and decrease chromosomal aberrations induced by cyclophosphamide. Among three concentrations (100 mg, 150 mg and 200 mg per Kg Body Weight), the most effective conc. of extract was 200 mg/Kg Body Wt. It decreased significant level of numerical and structural aberrations (breaks, premature centromeric division etc. up to 88%, p avocado fruit can be utilized for making active chemoprotective ingredient for lowering the side effect of chemotherapy like cyclophosphamide in cancer therapy.

  13. Evaluación de un proyecto de inversión usando opciones reales para diferenciar el aguacate

    OpenAIRE

    Karina Valencia Sandoval

    2016-01-01

    El estudio se centra en la comparación de las ganancias obtenidas en el cultivo de aguacate (Persea americana Mill.) cuando se le añade mayor valor agregado: destacando sus propiedades funcionales, se integra a esta comparación la volatilidad de los precios del fruto. Se centra en las entidades de mayor producción: Michoacán, Morelos, Nayarit, México, Jalisco, Puebla y Yucatán. La metodología se fundamenta tanto en evaluaciones tradicionales (VAN) como en el uso de árboles binomiales y fórmul...

  14. In vitro evaluation of genotoxicity of avocado (Persea americana) fruit and leaf extracts in human peripheral lymphocytes.

    Science.gov (United States)

    Kulkarni, Paresh; Paul, Rajkumar; Ganesh, N

    2010-07-01

    Persea americana is much sought after both for the nutritional value of its fruit and the medicinal values of its various plant parts. A chromosomal aberration assay was undertaken to evaluate the potential genotoxicity of crude extracts from avocado fruits and leaves. Chromosomal aberrations were observed in cultured human peripheral lymphocytes exposed to separately increasing concentrations of 50% methanolic extracts of Persea americana fruit and leaves. The groups exposed to leaf and fruit extracts, respectively, showed a concentration-dependent increase in chromosomal aberrations as compared to that in a control group. The mean percentage total aberrant metaphases at 100 mg/kg, 200 mg/kg, and 300 mg/kg concentrations of leaf extract were found respectively to be 58 ± 7.05, 72 ± 6.41, and 78 ± 5.98, which were significantly higher (p < 0.0001 each) than that in the control group (6 ± 3.39). The mean percentage total aberrant metaphases at 100 mg/kg, 200 mg/kg, and 300 mg/kg concentrations of fruit extract were found to be 18 ± 5.49, 40 ± 10.00, and 52 ± 10.20, respectively, which were significantly higher (p = 0.033, p < 0.0001, and p < 0.0001, respectively) than that for control (6 ± 3.39). Acrocentric associations and premature centromeric separation were the two most common abnormalities observed in both the exposed groups. The group exposed to leaf extracts also showed a significant number of a variety of other structural aberrations, including breaks, fragments, dicentrics, terminal deletion, minutes, and Robertsonian translocations. The group exposed to leaf extract showed higher frequency of all types of aberrations at equal concentrations as compared to the group exposed to fruit extract.

  15. Generation of composite Persea americana (Mill.) (avocado) plants: A proof-of-concept-study.

    Science.gov (United States)

    Prabhu, S Ashok; Ndlovu, Buyani; Engelbrecht, Juanita; van den Berg, Noëlani

    2017-01-01

    Avocado (Persea americana (Mill.)), an important commercial fruit, is severely affected by Phytophthora Root Rot in areas where the pathogen is prevalent. However, advances in molecular research are hindered by the lack of a high-throughput transient transformation system in this non-model plant. In this study, a proof-of-concept is demonstrated by the successful application of Agrobacterium rhizogenes-mediated plant transformation to produce composite avocado plants. Two ex vitro strategies were assessed on two avocado genotypes (Itzamna and A0.74): In the first approach, 8-week-old etiolated seedlings were scarred with a sterile hacksaw blade at the base of the shoot, and in the second, inch-long incisions were made at the base of the shoot (20-week-old non-etiolated plants) with a sterile blade to remove the cortical tissue. The scarred/wounded shoot surfaces were treated with A. rhizogenes strains (K599 or ARqua1) transformed with or without binary plant transformation vectors pRedRootII (DsRed1 marker), pBYR2e1-GFP (GFP- green fluorescence protein marker) or pBINUbiGUSint (GUS- beta-glucuronidase marker) with and without rooting hormone (Dip 'N' Grow) application. The treated shoot regions were air-layered with sterile moist cocopeat to induce root formation. Results showed that hormone application significantly increased root induction, while Agrobacterium-only treatments resulted in very few roots. Combination treatments of hormone+Agrobacterium (-/+ plasmids) showed no significant difference. Only the ARqua1(+plasmid):A0.74 combination resulted in root transformants, with hormone+ARqua1(+pBINUbiGUSint) being the most effective treatment with ~17 and 25% composite plants resulting from strategy-1 and strategy-2, respectively. GUS- and GFP-expressing roots accounted for less than 4 and ~11%, respectively, of the total roots/treatment/avocado genotype. The average number of transgenic roots on the composite plants was less than one per plant in all treatments

  16. PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF SELECTED MANGO (MANGIFERA INDICA L.) AND AVOCADO (PERSEA AMERICANA) FRUITS IN ILLU ABABOR ZONE, OROMIA REGIONAL STATE, ETHIOPIA

    OpenAIRE

    Lalisa Wakjira Duresa1*, Daniel Manaye2

    2017-01-01

    Mango (Mangifera indica L.) and Avocado (Persea americana) fruits are rich in bioactive molecules that protect human cells against the detrimental effect of free radicals. The phytochemical analysis revealed the Presence of alkaloids, terpenoids, saponins, tannins, phenolics and flavonoids in both fruits. The free radical scavenging activity using total antioxidant capacity of the phosphomolybdenum method and hydrogen peroxide method were carried out on the water extracts of mango and avocado...

  17. EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi

    Directory of Open Access Journals (Sweden)

    Mahomed Waheed

    2011-11-01

    Full Text Available Abstract Background Avocado (Persea americana belongs to the Lauraceae family and is an important commercial fruit crop in over 50 countries. The most serious pathogen affecting avocado production is Phytophthora cinnamomi which causes Phytophthora root rot (PRR. Root pathogens such as P. cinnamomi and their interactions with hosts are poorly understood and despite the importance of both the avocado crop and the effect Phytophthora has on its cultivation, there is a lack of molecular knowledge underpinning our understanding of defence strategies against the pathogen. In order to initiate a better understanding of host-specific defence we have generated EST data using 454 pyrosequencing and profiled nine defence-related genes from Pc-infected avocado roots. Results 2.0 Mb of data was generated consisting of ~10,000 reads on a single lane of the GS FLX platform. Using the Newbler assembler 371 contigs were assembled, of which 367 are novel for Persea americana. Genes were classified according to Gene Ontology terms. In addition to identifying root-specific ESTs we were also able to identify and quantify the expression of nine defence-related genes that were differentially regulated in response to P. cinnamomi. Genes such as metallothionein, thaumatin and the pathogenesis related PsemI, mlo and profilin were found to be differentially regulated. Conclusions This is the first study in elucidating the avocado root transcriptome as well as identifying defence responses of avocado roots to the root pathogen P. cinnamomi. Our data is currently the only EST data that has been generated for avocado rootstocks, and the ESTs identified in this study have already been useful in identifying defence-related genes as well as providing gene information for other studies looking at processes such as ROS regulation as well as hypoxia in avocado roots. Our EST data will aid in the elucidation of the avocado transcriptome and identification of markers for improved

  18. Persea americana Glycolic Extract: In Vitro Study of Antimicrobial Activity against Candida albicans Biofilm and Cytotoxicity Evaluation

    Directory of Open Access Journals (Sweden)

    D. Jesus

    2015-01-01

    Full Text Available This study evaluated the antifungal activity of Persea americana extract on Candida albicans biofilm and its cytotoxicity in macrophage culture (RAW 264.7. To determine the minimum inhibitory concentration (MIC, microdilution in broth (CLSI M27-S4 protocol was performed. Thereafter, the concentrations of 12.5, 25, 50, 100, and 200 mg/mL (n=10 with 5 min exposure were analyzed on mature biofilm in microplate wells for 48 h. Saline was used as control (n=10. After treatment, biofilm cells were scraped off and dilutions were plated on Sabouraud dextrose agar. After incubation (37°C/48 h, the values of colony forming units per milliliter (CFU/mL were converted to log10 and analyzed (ANOVA and Tukey test, 5%. The cytotoxicity of the P. americana extract was evaluated on macrophages by MTT assay. The MIC of the extract was 6.25 mg/mL and with 12.5 mg/mL there was elimination of 100% of planktonic cultures. Regarding the biofilms, a significant reduction (P<0.001 of the biofilm at concentrations of 50 (0.580±0.209 log10, 100 (0.998±0.508 log10, and 200 mg/mL (1.093±0.462 log10 was observed. The concentrations of 200 and 100 mg/mL were cytotoxic for macrophages, while the concentrations of 50, 25, and 12.5 mg/mL showed viability higher than 55%.

  19. Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro.

    Science.gov (United States)

    Oboh, Ganiyu; Odubanjo, Veronica O; Bello, Fatai; Ademosun, Ayokunle O; Oyeleye, Sunday I; Nwanna, Emem E; Ademiluyi, Adedayo O

    2016-03-01

    Avocado pear (Persea americana Mill.) leaves and seeds are used in traditional medicine for the treatment/management of Alzheimer disease (AD); however, information on the mechanism of actions is limited. This study sought to investigate the effect of P. americana leaf and seed aqueous extracts on some enzymes linked with AD (acetylcholinesterase [AChE] and butyrylcholinesterase [BChE] activities) and their antioxidant potentials in vitro. The inhibitory effects of extracts on AChE and BChE activities and antioxidant potentials (inhibition of Fe2+- and sodium nitroprusside-induced thiobarbiturate reactive species [TBARS] production in rat brain homogenates, radicals [1,1-diphenyl-2-picrylhydrazyl, hydroxyl, and nitric oxide] scavenging and iron [Fe] chelation abilities) were investigated. Phenolic content and phytochemical screening were carried out. Alkaloid profile was also determined using gas chromatography coupled with flame ionization detector (GC-FID). The extracts inhibited AChE and BChE activities and prooxidant-induced TBARS production in a dose-dependent manner, with the seed extract having the highest inhibitory effect and the leaf extract exhibiting higher phenolic content and radical scavenging abilities, but lower Fe chelation ability compared with that of the seed. The phytochemical screening revealed the presence of saponins, alkaloids, and terpenoids in both extracts, whereas the total alkaloid profile was higher in the seed extract than in the leaf extract, as revealed by GC-FID. The anti-cholinesterase and antioxidant activities of avocado leaf and seed could be linked to their phytoconstituents and might be the possible mechanisms underlying their use as a cheap and natural treatment/management of AD. However, these extracts should be further investigated in vivo.

  20. Integrating gene flow, crop biology, and farm management in on-farm conservation of avocado (Persea americana, Lauraceae).

    Science.gov (United States)

    Birnbaum, Kenneth; Desalle, Rob; Peters, Charles M; Benfey, Philip N

    2003-11-01

    Maintaining crop diversity on farms where cultivars can evolve is a conservation goal, but few tools are available to assess the long-term maintenance of genetic diversity on farms. One important issue for on-farm conservation is gene flow from crops with a narrow genetic base into related populations that are genetically diverse. In a case study of avocado (Persea americana var. americana) in one of its centers of diversity (San Jerónimo, Costa Rica), we used 10 DNA microsatellite markers in a parentage analysis to estimate gene flow from commercialized varieties into a traditional crop population. Five commercialized genotypes comprised nearly 40% of orchard trees, but they contributed only about 14.5% of the gametes to the youngest cohort of trees. Although commercialized varieties and the diverse population were often planted on the same farm, planting patterns appeared to keep the two types of trees separated on small scales, possibly explaining the limited gene flow. In a simulation that combined gene flow estimates, crop biology, and graft tree management, loss of allelic diversity was less than 10% over 150 yr, and selection was effective in retaining desirable alleles in the diverse subpopulation. Simulations also showed that, in addition to gene flow, managing the genetic makeup and life history traits of the invasive commercialized varieties could have a significant impact on genetic diversity in the target population. The results support the feasibility of on-farm crop conservation, but simulations also showed that higher levels of gene flow could lead to severe losses of genetic diversity even if farmers continue to plant diverse varieties.

  1. Alguns aspectos da anatomía-ecológica de Persea americana Mili (abacateiro e interpretações fisio-ecológicas

    Directory of Open Access Journals (Sweden)

    Moacyr Eurípedes Medri

    1985-11-01

    Full Text Available In the present work are presented anatomical differences in leaves situaded in different strata on the same tree ofPersea Americana. Analysis of linear regression and tests of coeficience of correlation demonstrate that in respect tb the relationships of the anatomical parameters studied, there is much interdependence. The histological point of view compares such parameters as number and size of stomata, volume of mesophyll, area of functional xylem, phloem, supportive tissue and epidermic tissue, and on the other han different microclimatic positions, domonstrates that Persea Americana is an extremely plastic species with much adaptability; as plastic as Hevea brasiliensis Muelli. Arg and much more plastic than Bertholletia excelsa Humb. & Benpl.No presente trabalho são apresentadas as diferenças anatômicas em folhas situadas em diferentes estratos de uma mesma árvore de Persea americana MUI, mostrando através de análise de regressão linear, coeficiente de correlação conclusivos a, respeito das relações diretas e significativas no tocante aos parâmetros anatômicos estudados, evidenciando interdependências dos mesmos. Do ponto de vista histológico, onde se tomou como parâmetro o número e tamanho de estômatos e volume do mesófilo, área xilemática útil, floema, tecido mecânico e epidérmico, e de outro lado, condições microclimáticas diferentes, P. americana evidencia ser uma espécie bastante plástica, havendo uma relação direta se correlacionada ao seu amplo comportamento adaptativo, sendo tão plástica quanto Hevea brasiliensis Muell. Arg. (Medri, 1977 e muito mais que Bertholletia excelsa Humb. & Benpl. (Medri, 1978.

  2. Effects of Persea americana leaf extracts on body weight and liver ...

    African Journals Online (AJOL)

    GREGO

    2007-04-16

    Apr 16, 2007 ... weight of either aqueous or methanolic extract of P. americana leaf daily for 8 weeks. There were no significant differences (p>0.05) in the overall body weight gain of the hyperlipidaemic rats compared to normal control. However, the administration of the aqueous and methanolic extracts provoked 14 and.

  3. MORPHOANATOMICAL LEAF ANALYSIS IN HORTICULTURAL GROUPS OF AVOCADO (Persea americana PLACED AT INIA-CENIAP’S COLLECTION, VENEZUELA

    Directory of Open Access Journals (Sweden)

    H. Ferrer Pereira

    2017-08-01

    Full Text Available The avocado (Persea americana Mill. is the most important species of Lauraceae in America due to its exploitation as food for pre-Columbian and modern cultures. It is a very important seasonal crop in Venezuela based on a perennial fruit tree management. From a selection of 76 accessions (45 cultivars of avocados cultivated at the Germplasm Bank of INIA-CENIAP, a morphoanatomical analysis was performed to identify attributes of taxonomic resolution (diagnostic characters which allow to characterize sets and / or culta. Morphological study was carried out from each accession herborized sample. Information was obtained by freehand transverse leaf sections (epidermis, mesophyll and midvein as well as paradermic preparations, and observed data was recorded in DELTA System. New morphoanatomical characters and discriminating attributes between cultivars were identified and described, especially to discriminate the Mexican group, and a close relationship within West Indian and Guatemalan cultivars was observed due to the variability identified from the latter group. Indument- related attributes were highly informative to discriminate among cultivars, along with the outline, apical angle and projections at the base of the leaf blades, stem cross section and presence of anise odor, progress and joining of the secondary nerve branches, tertiary venation pattern, abaxial contour and thickness of the sclerenchymatous sheath and compaction of the phloem in the vascular bundle, adaxial contour of the median nerve, and thickness, outline and uniformity of the anticlinal walls of adaxial and abaxial epidermal cells.

  4. THE USE OF POLYSACCHARIDES EXTRACTED FROM SEED OF Persea americana var. Hass ON THE SYNTHESIS OF ACRYLIC HYDROGELS

    Directory of Open Access Journals (Sweden)

    Vicente Arturo Lara-Valencia

    Full Text Available This paper reports the use of polysaccharides extracted from seed of Persea americana var. Hass in the synthesis of acrylic hydrogels. The effects of the chemical composition (acrylamide/acrylic acid, the concentration of crosslinking agent (glycerol diacrylate and the type of initiation (redox, photoinitiation of the hydrogels were evaluated with and without polysaccharides. Xerogels were characterized by FTIR spectroscopy, differential scanning calorimetry (DSC and scanning electron microscopy (SEM, while for the swollen hydrogels the swelling kinetic and mechanical properties were evaluated. The kinetic parameters were obtained using the second order equation proposed by Schott, where it is reported that by increasing the concentration of the crosslinking agent, the degree of swelling is reduced because of the greater structural level. The increase of the amount of acrylamide and the amount of polysaccharides causes also a decrease in the swelling degree. The type of initiation also affected the hydrogels swelling kinetic, the photoinitiated hydrogels were the ones that captured less water. Moreover, the increasing of the glass transition temperature and the compression modulus with the crosslinking agent concentration and molar ratio AAm/AAc are observed for hydrogels with and without polysaccharides. The results demonstrate a successful incorporation of polysaccharides into the polymeric network.

  5. Profiling LC-DAD-ESI-TOF MS method for the determination of phenolic metabolites from avocado (Persea americana).

    Science.gov (United States)

    Hurtado-Fernández, Elena; Carrasco-Pancorbo, Alegría; Fernández-Gutiérrez, Alberto

    2011-03-23

    A powerful HPLC-DAD-ESI-TOF MS method was established for the efficient identification of the chemical constituents in the methanolic extracts of avocado (Persea americana). Separation and detection conditions were optimized by using a standard mix containing 39 compounds belonging to phenolic acids and different categories of flavonoids, analytes that could be potentially present in the avocado extracts. Optimum LC separation was achieved on a Zorbax Eclipse Plus C18 analytical column (4.6×150 mm, 1.8 μm particle size) by gradient elution with water+acetic acid (0.5%) and acetonitrile as mobile phases, at a flow rate of 1.6 mL/min. The detection was carried out by ultraviolet-visible absorption and ESI-TOF MS. The developed method was applied to the study of 3 different varieties of avocado, and 17 compounds were unequivocally identified with standards. Moreover, around 25 analytes were tentatively identified by taking into account the accuracy and isotopic information provided by TOF MS.

  6. Hepatoprotective properties of aqueous leaf extract of Persea Americana, Mill (Lauraceae) 'avocado' against CCL4-induced damage in rats.

    Science.gov (United States)

    Brai, Bartholomew I C; Adisa, Rahmat A; Odetola, Adebimpe A

    2014-01-01

    Natural products from plants have received considerable attention in recent years due to their diverse pharmacological properties, including antioxidants and hepatoprotective activities. The protective effects of aqueous extract of Persea americana (AEPA) against carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats was investigated. Liver damage was induced in rats by administering a 1:1 (v/v) mixture of CCl4 and olive oil [3 ml/kg, subcutaneously (sc)] after pre-treatment for 7 days with AEPA. Hepatoprotective effects of AEPA was evaluated by estimating the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and levels of total bilirubin (TBL). The effects of AEPA on biomarkers of oxidative damage (lipid peroxidation) and antioxidant enzymes namely, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST) were measured in liver post mitochondrial fraction. AEPA and Reducdyn® showed significant (p<0.05) hepatoprotective activity by decreasing the activities of ALT, AST, ALP and reducing the levels of TBL. The activities of antioxidant enzymes, levels of malondialdehyde and protein carbonyls were also decreased dose-dependently in the AEPA-treated rats. Pre-treatment with AEPA also decreased the serum levels of glutathione significantly. These data revealed that AEPA possesses significant hepatoprotective effects against CCl4-induced toxicity attributable to its constituent phytochemicals. The mechanism of hepatoprotection seems to be through modulation of antioxidant enzyme system.

  7. Isolation and chemical identification of lipid derivatives from avocado (Persea americana) pulp with antiplatelet and antithrombotic activities.

    Science.gov (United States)

    Rodriguez-Sanchez, Dariana Graciela; Flores-García, Mirthala; Silva-Platas, Christian; Rizzo, Sheryl; Torre-Amione, Guillermo; De la Peña-Diaz, Aurora; Hernández-Brenes, Carmen; García-Rivas, Gerardo

    2015-01-01

    Platelets play a pivotal role in physiological hemostasis. However, in coronary arteries damaged by atherosclerosis, enhanced platelet aggregation, with subsequent thrombus formation, is a precipitating factor in acute ischemic events. Avocado pulp (Persea americana) is a good source of bioactive compounds, and its inclusion in the diet as a source of fatty acid has been related to reduced platelet aggregability. Nevertheless, constituents of avocado pulp with antiplatelet activity remain unknown. The present study aims to characterize the chemical nature of avocado constituents with inhibitory effects on platelet aggregation. Centrifugal partition chromatography (CPC) was used as a fractionation and purification tool, guided by an in vitro adenosine diphosphate (ADP), arachidonic acid or collagen-platelet aggregation assay. Antiplatelet activity was initially linked to seven acetogenins that were further purified, and their dose-dependent effects in the presence of various agonists were contrasted. This process led to the identification of Persenone-C (3) as the most potent antiplatelet acetogenin (IC₅₀=3.4 mM) among the evaluated compounds. In vivo evaluations with Persenone A (4) demonstrated potential protective effects against arterial thrombosis (25 mg kg⁻¹ of body weight), as coagulation times increased (2-fold with respect to the vehicle) and thrombus formation was attenuated (71% versus vehicle). From these results, avocado may be referred to as a functional food containing acetogenin compounds that inhibit platelet aggregation with a potential preventive effect on thrombus formation, such as those that occur in ischaemic diseases.

  8. Impact of Laurel Wilt, Caused by Raffaelea lauricola, on Leaf Gas Exchange and Xylem Sap Flow in Avocado, Persea americana.

    Science.gov (United States)

    Ploetz, Randy C; Schaffer, Bruce; Vargas, Ana I; Konkol, Joshua L; Salvatierra, Juanpablo; Wideman, Ronney

    2015-04-01

    Laurel wilt, caused by Raffaelea lauricola, is a destructive disease of avocado (Persea americana). The susceptibility of different cultivars and races was examined previously but more information is needed on how this host responds to the disease. In the present study, net CO2 assimilation (A), stomatal conductance of H2O (gs), transpiration (E), water use efficiency (WUE), and xylem sap flow rates were assessed in cultivars that differed in susceptibility. After artificial inoculation with R. lauricola, there was a close relationship between symptom development and reductions in A, gs, E, WUE, and mean daily sap flow in the most susceptible cultivar, 'Russell', and significantly greater disease and lower A, gs, E, WUE, and sap flow rates were usually detected after 15 days compared with the more tolerant 'Brogdon' and 'Marcus Pumpkin'. Significant differences in preinoculation A, gs, E, and WUE were generally not detected among the cultivars but preinoculation sap flow rates were greater in Russell than in Brogdon and Marcus Pumpkin. Preinoculation sap flow rates and symptom severity for individual trees were correlated at the end of an experiment (r=0.46), indicating that a plant's susceptibility to laurel wilt was related to its ability to conduct water. The potential management of this disease with clonal rootstocks that reduce sap flow rates is discussed.

  9. Effect of heat treatment oanas irradiation, and combined treatment on the shelf of fresh avocados (Persea americana L)

    International Nuclear Information System (INIS)

    Purwanto, Z.I.; Maha, Munsiah

    1987-01-01

    Effects of heat treatment, gamma irradiation, and combined treatments on the shelf-life of fresh avocados (Persea americana L.). Experiments to determine the effective irradiation conditions to prolong the shelf-life of fresh avocados were conducted at the centre for the Application of Isotopes washed and dried, then divided into 4 groups, namely for control (A), dipped in hot water at 40 0 C for 20 minutes (heat treatment, B), irradiated at a dose of 25 Gy (C), and combination of hot water dipping (40 0 C for 20 minutes) and irradiation at a 25 Gy (D). The samples were stored at room temperature. Evaluation on physical and chemical properties of the samples was done daily up to 10 days' storage. Parameters observed were texture, moisture and fat contents, percentage of weight loss, percentage of mature fruit, and subjective organoleptic evaluation. It was found that treated samples with and without combination treatment were still in good condition until 10 days of storage. It could be concluded that dipping in hot water, either alone or in combination with irradiation at a dose of 25 Gy could extend the shelf-life of fresh avocado up to 10 days at room temperature compared to only 5 days of the control. (author). 2 figs, 8 refs

  10. Identification of avocado (Persea americana) pulp proteins by nano-LC-MS/MS via combinatorial peptide ligand libraries.

    Science.gov (United States)

    Esteve, Clara; D'Amato, Alfonsina; Marina, María Luisa; García, María Concepción; Righetti, Pier Giorgio

    2012-09-01

    Avocado (Persea americana) proteins have been scarcely studied despite their importance, especially in food related allergies. The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries at pH 7.4 and under conditions mimicking reverse-phase capture at pH 2.2. The total number of unique gene products identified amounts to 1012 proteins, of which 174 are in common with the control, untreated sample, 190 are present only in the control and 648 represent the new species detected via combinatorial peptide ligand libraries of all combined eluates and likely represent low-abundance proteins. Among the 1012 proteins, it was possible to identify the already known avocado allergen Pers a 1 and different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin-like protein, a glucanase, and an isoflavone reductase like protein. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Métodos para atrair a abelha Apis mellifera L. em cultura de abacate (Persea americana Mill. Methods to attract honeybee Apis mellifera L. to avocado tree (Persea americana Mill

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    Darclet Terezinha Malerbo Souza

    2002-04-01

    Full Text Available O presente experimento teve como objetivo avaliar métodos de atração da abelha Apis mellifera L. (Hymenoptera, Apidae em duas variedades de abacate (Persea americana Mill.. Os atrativos utilizados foram extratos de Cymbopogon citratus, Ocimum basilicum, Lippia alba, folha de Citrus sp, folha de Eucaliptus sp. e o eugenol e o linalol (SIGMA. Os tratamentos utilizados foram: coberto; descoberto pulverizado (DP; descoberto com tubos e descoberto não pulverizado (DNP. Observou-se que a atratividade das substâncias testadas desapareceu minutos após a sua aplicação, utilizando ou não a glicerina, em ambas as variedades. A pulverização dos extratos de falsa melissa, folhas de eucalipto e folhas de laranja apresentaram um aumento no número de abelhas Apis mellifera, na variedade Quintal. Os dados mostraram que a freqüência das abelhas A. mellifera foi maior na variedade Quintal comparada à variedade Fortuna. Isto pode ter ocorrido devido à maior concentração de açúcares do néctar de suas flores. Observou-se que as abelhas A. mellifera preferiram visitar as flores do abacateiro da variedade Quintal, tanto para néctar quanto para coleta de pólen, comparada à variedade Fortuna. Com relação aos frutos, nenhuma das características apresentou diferença significativa entre os tratamentos, em ambas as variedades. Entretanto, observou-se que na variedade Quintal os frutos decorrentes dos tratamentos DP e DNP foram mais pesados, mais compridos e com maior espessura da polpa, comparados ao único fruto obtido do tratamento coberto. Os produtos testados em tubos não foram eficientes para atrair a abelha Apis mellifera, em ambas as variedades.The present experiment was carried out to evaluate some methods to attract honeybee Apis mellifera L. (Hymenoptera, Apidae to two avocado varieties (Persea americana Mill.. Extracts of Cymbopogon citratus, Ocimum basilicum, Lippia alba, Citrus sp leaf, Eucalyptus sp leaf, the eugenol and linalol

  12. Cinética de inativação da polifenoloxidase e peroxidase de abacate (Persea americana Mill. Kinetic of inactivation of polyphenoloxidase and peroxidase of avocado (Persea americana Mill.

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    Rúbia Caroline Luíz

    2007-12-01

    Full Text Available Extratos enzimáticos foram preparados a partir da polpa de abacate (Persea americana Mill. dos cultivares Quintal, Fortuna e Choquete, em dois estádios de maturação (verde e maduro. A polpa de abacate (150,00g foi homogeneizada em liquidificador, com 300 mL de solução tampão fosfato de sódio 100mM em pH 7,4 para polifenoloxidase (PPO e pH 6,0 para peroxidase (POD. A peroxidase ionicamente ligada foi extraída usando solução de NaCl 1,0M, em tampão fosfato de sódio 100mM pH 6,0. Para o estudo da estabilidade térmica, os extratos foram submetidos a temperaturas de 60, 65, 70, 75 e 80ºC e por períodos que variaram de 1 à 10 minutos e a atividade enzimática foi determinada por espectrofotometria (lambda= 395nm para PPO e lambda= 460nm para POD. Pode-se observar que o declínio rápido da atividade das enzimas era maior nos primeiros quatro minutos e após esse período, com o aumento da temperatura e do tempo, a atividade continuou diminuindo, porém de forma mais lenta. A energia de ativação na faixa de temperatura estudada para as porções termolábil e termorresistente, apresentou valores dentro da faixa 12-100 kcal/mol.Enzymatic extracts were prepared from the avocado pulp (Persea americana Mill., using the cultivars Quintal, Fortuna and Choquete, in two different maturation stages (green and mature avocados. The avocado pulp (150,00g was homogenized in blender with 300 mL of sodium phosphate buffer 100mM at pH 7.4 for polyphenoloxidase (PPO and pH 6.0 for peroxidase (POD. The peroxidase ionically bound was extracted using NaCl solution 1.0M in sodium phosphate buffer 100mM pH 6.0. For the study of the thermal stability the extracts were submitted at temperatures of 60, 65, 70, 75 and 80ºC for periods that had a variation from 1 to 10 minutes and the enzymatic activity was determined using a spectrophotometer (lambda= 395nm for PPO and lambda= 460nm for POD. It was possible to observe a fast decline of the enzyme activity in

  13. Transcriptome responses of an ungrafted Phytophthora root rot tolerant avocado (Persea americana) rootstock to flooding and Phytophthora cinnamomi.

    Science.gov (United States)

    Reeksting, B J; Olivier, N A; van den Berg, N

    2016-09-22

    Avocado (Persea americana Mill.) is a commercially important fruit crop worldwide. A major limitation to production is the oomycete Phytophthora cinnamomi, which causes root rot leading to branch-dieback and tree death. The decline of orchards infected with P. cinnamomi occurs much faster when exposed to flooding, even if flooding is only transient. Flooding is a multifactorial stress compromised of several individual stresses, making breeding and selection for tolerant varieties challenging. With more plantations occurring in marginal areas, with imperfect irrigation and drainage, understanding the response of avocado to these stresses will be important for the industry. Maintenance of energy production was found to be central in the response to flooding, as seen by up-regulation of transcripts related to glycolysis and induction of transcripts related to ethanolic fermentation. Energy-intensive processes were generally down-regulated, as evidenced by repression of transcripts related to processes such as secondary cell-wall biosynthesis as well as defence-related transcripts. Aquaporins were found to be down-regulated in avocado roots exposed to flooding, indicating reduced water-uptake under these conditions. The transcriptomic response of avocado to flooding and P. cinnamomi was investigated utilizing microarray analysis. Differences in the transcriptome caused by the presence of the pathogen were minor compared to transcriptomic perturbations caused by flooding. The transcriptomic response of avocado to flooding reveals a response to flooding that is conserved in several species. This data could provide key information that could be used to improve selection of stress tolerant rootstocks in the avocado industry.

  14. Evaluación de aguacates criollos en Nuevo León, México: región sur Evaluation of Creole avocados in Nuevo León, Mexico: southern region

    OpenAIRE

    Efraín Acosta Díaz; Ismael Hernández Torres; Isidro Humberto Almeyda León

    2012-01-01

    El estado de Nuevo León, México, forma parte del centro de origen de la raza mexicana de aguacate (Persea americana Mill. var. drymifolia), en donde se cultivan variedades criollas en huertos comerciales y en traspatios, predominando la utilización de la variedad Plátano Grueso-1. Esta variedad se caracteriza por presentar un ciclo temprano de madurez fisiológica, lo cual permite que su producción se concentre en un periodo relativamente corto. El objetivo de esta investigación fue caracteriz...

  15. THE EFFECTIVENESS OF 70% METHANOLIC EXTRACT OF AVOCADO LEAF (Persea americana Mill IN DECREASING BLOOD SUGAR LEVELS IN MALE RATS (Rattus norvegicus WISTAR STRAIN INDUCED ALLOXAN

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    Retno Sintowati

    2017-01-01

    Metode penelitian menggunakan eksperimental laboratorik dengan rancangan penelitian pretest – posttest with control group design. Hewan uji yang digunakan 25 ekor tikus jantan galur Wistar, umur 2-3 bulan, dan berat badan 150-200 gram yang dibagi dalam 5 kelompok. Kelompok I: kontrol negatif (aquades, kelompok II: kontrol positif (glibenklamid, kelompok III, IV, V : ekstrak metanol 70% daun alpukat dengan dosis berturut-turut adalah sebagbai berikut: 100 mg/ 200 grBB, 150 mg/ 200 grBB, 200 mg/ 200 grBB. Gula darah diukur dengan alat spektrofotometer. Berdasarkan hasil uji ANOVA, data penurunan kadar gula darah hari ke-7 pemberian ekstrak nilai probabilitas signifikan p = 0, 000 dengan demikian p < 0, 05 maka terdapat perbedaan penurunan kadar gula darah yang bermakna. Berdasarkan uji LSD dari semua kelompok didapatkan nilai p < 0,05 yang berarti terdapat perbedaan penurunan kadar gula darah yang signifikan antara2 kelompok. Kesimpulan: Ekstrak metanol 70% daun alpukat (Persea americana Mill dosis 200 mg/200; 100 mg/200 and 150 mg/ 200 gram/berat badan dapat menurunkan kadar gula darah pada mencit jantan galur wistar Kata Kunci : Ekstrak metanol 70%, Alpukat (Persea americana Mill, gula darah, diabetes mellitus.

  16. Biochemical Effects of Aqueous Extract of Persea americana (Mill) on the Myocardium of Left Ventricle of High Salt-Fed Adult Wistar Rats.

    Science.gov (United States)

    Olushola, Ayoola I; Aderibigbe, Komolafe O; Stephen, Saka O; Ayodeji, Odukoya S

    2017-10-01

    The cardioprotective effects of Persea americana extract was investigated on biochemical activities of high salt-fed adult Wistar rats in this study. Forty healthy Wistar rats of both sexes weighing 120 to 150 g were randomly assigned into 8 groups of 5 rats each (groups A, B, C, D, E, F, G, and H). Rats in groups A, F, G, and H were fed with standard laboratory pellets, while groups B, C, D, and E were fed on the high-salt diet for 4 weeks. Concomitantly, daily administration of 50, 100, and 150 mg/kg of the P americana extract were given orally to groups C and F, D and G, and E and H, respectively, while rats in groups A and B were administered distilled water. Blood samples were taken by cardiac puncture; concentration of sodium ion, potassium ion, nitric oxide, and activity of lactate dehydrogenase were determined. One-way analysis of variance was used to analyze data, followed by Student-Newman-Keuls (SNK) test for multiple comparison. Results revealed that concentration of potassium ion and nitric oxide was significantly lower ( P < .05) in high salt-fed groups. Sodium ion concentration and activity of lactate dehydrogenase were higher in high salt-fed group while P americana prevented biochemical perturbations in other experimental groups. In conclusion, high salt-diet induced biochemical alterations which were significantly protected by oral administration of P americana extract.

  17. Stability of a colloidal system based on avocado (Persea americana Mill. cv. Hass and others: Effect of process and composition

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    Eliana María Estrada Mesa

    2017-07-01

    Full Text Available Avocado (Persea americana Mill. cv. Hass, is a perishable fruit, which compositionally presents nutritional benefits, which has led to its productive chain to look for new alternatives of agroindustrialization to improve its competitiveness in the market, being guacamole a potential option. The aim of this research was to evaluate the influence of composition and homogenization process on the colloidal system stability based on avocado and other ingredients for a potential use in the aspersion drying process. We used the surface response methodology and a central composite experimental design for experimental optimization of the process, considering three independent variables as follows: avocado dry solids (DSavocado, homogenization time and tert-butylhydroquinone concentration (TBHQ, and six dependent variables: zeta potential (-ζ, color (L *: brightness, a *: green-red chromaticity, b *: yellow-blue chromaticity, viscosity (μ, spectral absorption stability index, peroxide index (PI and particle size (D10, D50 and D90. The best emulsion and process formulation was achieved with 47.1% of DSavocado, 5 min of homogenization and 100 mg.kg-1 of TBHQ, obtaining an emulsion with -ζ = -27.67 ± 0.29, L * = 51.3 ± 1.0, a * = -5.8 ± 0.8 and b * = 30.0 ± 1.9, μ = 1034.56 ± 95.91 cP , R = 0.78 ± 0.03, IP = 0.73 ± 0.30 meq H2O2.Kg-1 emulsion, D10 = 8.1 ± 0.7 μm, D50 = 56.2 ± 11.5 μm and D90 = 346.6 ± 94.6 μm. The emulsion based on avocado and other ingredients, presents a physico-chemical, physical stability adequate stability, which guarantees a potential use for aspersion-drying process, since it meets with the criteria design for viscosity and total solids, of the pilot unit PSALAB, Vibrasec S.A.

  18. Using Worldview Satellite Imagery to Map Yield in Avocado (Persea americana: A Case Study in Bundaberg, Australia

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    Andrew Robson

    2017-11-01

    Full Text Available Accurate pre-harvest estimation of avocado (Persea americana cv. Haas yield offers a range of benefits to industry and growers. Currently there is no commercial yield monitor available for avocado tree crops and the manual count method used for yield forecasting can be highly inaccurate. Remote sensing using satellite imagery offers a potential means to achieve accurate pre-harvest yield forecasting. This study evaluated the accuracies of high resolution WorldView (WV 2 and 3 satellite imagery and targeted field sampling for the pre-harvest prediction of total fruit weight (kg·tree−1 and average fruit size (g and for mapping the spatial distribution of these yield parameters across the orchard block. WV 2 satellite imagery was acquired over two avocado orchards during 2014, and WV3 imagery was acquired in 2016 and 2017 over these same two orchards plus an additional three orchards. Sample trees representing high, medium and low vigour zones were selected from normalised difference vegetation index (NDVI derived from the WV images and sampled for total fruit weight (kg·tree−1 and average fruit size (g per tree. For each sample tree, spectral reflectance data was extracted from the eight band multispectral WV imagery and 18 vegetation indices (VIs derived. Principal component analysis (PCA and non-linear regression analysis was applied to each of the derived VIs to determine the index with the strongest relationship to the measured total fruit weight and average fruit size. For all trees measured over the three year period (2014, 2016, and 2017 a consistent positive relationship was identified between the VI using near infrared band one and the red edge band (RENDVI1 to both total fruit weight (kg·tree−1 (R2 = 0.45, 0.28, and 0.29 respectively and average fruit size (g (R2 = 0.56, 0.37, and 0.29 respectively across all orchard blocks. Separate analysis of each orchard block produced higher R2 values as well as identifying different

  19. RECONOCIMIENTO E IDENTIFICACION DE TRIPS FITOFAGOS (THYSANOPTERA: THRIPIDAE Y DEPREDADORES (THYSANOPTERA: PHLAEOTHRIPIDAE ASOCIADOS A CULTIVOS COMERCIALES DE AGUACATE Persea spp. EN LOS DEPARTAMENTOS DE CALDAS Y RISARALDA (COLOMBIA RECOGNITION AND IDENTIFICATION OF PHYTOPHAGOUS THRIPS (THYSANOPTERA: THRIPIDAE AND PREDATORS (THYSANOPTERA: PHLAEOTHRIPIDAE ASSOCIATED WITH COMMERCIAL CULTIVATIONS OF AVOCADO Persea spp. IN THE DEPARTMENTS OF CALDAS AND RISARALDA, COLOMBIA

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    Fernando Echeverri Florez

    2004-06-01

    Full Text Available Se consideraron tres cultivos comerciales de aguacate (Persea sp. en los municipios de Palestina (Caldas y Pereira y Marsella (Risaralda evaluando árboles en producción entre cinco y ocho años de edad, incluyendo para los tres lugares las variedades: Santana, Choquette, Booth 8, Trinidad y Trapp; además en el huerto de Pereira se incluyó la variedad Fucsia, en las que se constataron los daños y distorsiones atribuidas a la acción de los trips. Para los materiales considerados se efectuaron diez muestreos por localidad con una frecuencia quincenal, escogiendo en cada muestreo un árbol por variedad. En cada árbol seleccionado se tomaron 36 muestras así: tres por estrato (alto, medio y bajo cuatro por punto cardinal (oriente occidente, norte y sur y tres estructuras de la planta (hojas tiernas, flores y frutos en desarrollo, para un total de 360 muestras por cada variedad de aguacate, por cada una de las tres localidades consideradas. A cada muestra se le extrajeron los trips, separando los morfos de acuerdo con la colección de referencia previamente establecida; se cuantificaron y se efectuó el proceso de identificación. En total se determinaron cuatro morfos asociados al cultivo de aguacate, dos de hábito fitófago: Selenothrips rubrocinctus Giard y Frankliniella gardeniae Moulton, según Mound, (1993 nuevo reporte como especie fitófaga asociada al aguacate y los géneros Leptothrips y Karnyothrips de hábito depredador. Se encontró para las tres localidades un amplio predominio de F. gardeniae con relación a los otros tres morfos, hospedándose principalmente en las estructuras florales, y en menor proporción en brotes tiernos y frutos en desarrollo. No se encontró preferencia de los trips por un punto específico con relación a la distribución vertical y horizontal en el dosel del árbol Se constataron los daños atribuidos a los trips en aguacate, encontrando en las plantaciones: En fruto: Pericarpio deforme, partenocarpia

  20. Evaluación de un proyecto de inversión usando opciones reales para diferenciar el aguacate

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    Karina Valencia Sandoval

    2016-01-01

    Full Text Available El estudio se centra en la comparación de las ganancias obtenidas en el cultivo de aguacate (Persea americana Mill. cuando se le añade mayor valor agregado: destacando sus propiedades funcionales, se integra a esta comparación la volatilidad de los precios del fruto. Se centra en las entidades de mayor producción: Michoacán, Morelos, Nayarit, México, Jalisco, Puebla y Yucatán. La metodología se fundamenta tanto en evaluaciones tradicionales (VAN como en el uso de árboles binomiales y fórmulas de Black-Scholes. Como conclusión, los resultados muestran que la relación benefi- cio-costo incrementa cuando se diferencia el producto, lo que permite a los proyectos una mayor rentabilidad, incluso para aquellos que se descartaron desde un principio mediante la valoración tradicional del VAN.

  1. Analysis of expressed sequence tags (ESTs) from avocado seed (Persea americana var. drymifolia) reveals abundant expression of the gene encoding the antimicrobial peptide snakin.

    Science.gov (United States)

    Guzmán-Rodríguez, Jaquelina J; Ibarra-Laclette, Enrique; Herrera-Estrella, Luis; Ochoa-Zarzosa, Alejandra; Suárez-Rodríguez, Luis María; Rodríguez-Zapata, Luis C; Salgado-Garciglia, Rafael; Jimenez-Moraila, Beatriz; López-Meza, Joel E; López-Gómez, Rodolfo

    2013-09-01

    Avocado is one of the most important fruits in the world. Avocado "native mexicano" (Persea americana var. drymifolia) seeds are widely used in the propagation of this plant and are the primary source of rootstocks globally for a variety of avocado cultivars, such as the Hass avocado. Here, we report the isolation of 5005 ESTs from the 5' ends of P. americana var. drymifolia seed cDNA clones representing 1584 possible unigenes. These avocado seed ESTs were compared with the avocado flower EST library, and we detected several genes that are expressed either in both tissues or only in the seed. The snakin gene, which encodes an element of the innate immune response in plants, was one of those most frequently found among the seed ESTs, and this suggests that it is abundantly expressed in the avocado seed. We expressed the snakin gene in a heterologous system, namely the bovine endothelial cell line BVE-E6E7. Conditioned media from transfected BVE-E6E7 cells showed antimicrobial activity against strains of Escherichia coli and Staphylococcus aureus. This is the first study of the function of the snakin gene in plant seed tissue, and our observations suggest that this gene might play a protective role in the avocado seed. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  2. Effect of Persea americana (avocado) fruit extract on the level of expression of adiponectin and PPAR-γ in rats subjected to experimental hyperlipidemia and obesity.

    Science.gov (United States)

    Padmanabhan, Monika; Arumugam, Geetha

    2014-06-01

    Persea americana, commonly known as avocado, is traditionally consumed fruit which possesses body fat lowering capacity. Adiponectin plays an important role in regulating obesity. In this study, the effect of hydro-alcoholic fruit extract of P. americana (HAEPA) on the level of blood lipids, glutathione, lipid peroxidation products, adiponectin and peroxisome proliferator activated receptor (PPAR)-γ expressions was investigated in rats fed a high-fat diet (HFD). Male Sprague Dawley rats were divided into four groups: groups 1 and 2 were fed normal rat chow (5% fat) and groups 3 and 4 were fed HFD (23% fat) for a period of 14 weeks. In addition, groups 2 and 4 rats were administered orally with 100 mg/kg body weight of HAEPA from third week. After 14 weeks, rats were sacrificed, and serum/plasma levels of total cholesterol, phospholipids, triglyceride, high-density lipoprotein (HDL), low-density lipoprotein (LDL) and adiponectin were determined. The mRNA expression of adiponectin, PPAR-γ and protein expression of PPAR-γ were also evaluated. The body mass index (BMI), total fat pad mass and adiposity index were significantly decreased in HAEPA co-administered rats than in HFD-fed rats. The levels of LDL and lipid peroxides were significantly higher in HFD group than in HFD+HAEPA group. Levels of reduced glutathione, adiponectin, mRNA expression of adiponectin, PPAR-γ and protein expression of PPAR-γ were found to be increased in HFD+HAEPA group than in HFD group. The hypolipidemic effect of HAEPA is also evidenced by the histological observations in liver, heart and adipose tissue. The results indicate that HAEPA exhibits hypolipidemic activity probably by increasing the mRNA expression of adiponectin and PPAR-γ, which reduce the risk of hyperlipidemia and obesity.

  3. Epidemiology, pathology and identification of Colletotrichum including a novel species associated with avocado (Persea americana) anthracnose in Israel.

    Science.gov (United States)

    Sharma, Gunjan; Maymon, Marcel; Freeman, Stanley

    2017-11-20

    Anthracnose disease caused by Colletotrichum species is a major constraint for the shelf-life and marketability of avocado fruits. To date, only C. gloeosporioides sensu lato and C. aenigma have been reported as pathogens affecting avocado in Israel. This study was conducted to identify and characterize Colletotrichum species associated with avocado anthracnose and to determine their survival on different host-structures in Israel. The pathogen survived and over-wintered mainly on fresh and dry leaves, as well as fresh twigs in the orchard. A collection of 538 Colletotrichum isolates used in this study was initially characterized based on morphology and banding patterns generated according to arbitrarily primed PCR to assess the genetic diversity of the fungal populations. Thereafter, based on multi-locus phylogenetic analyses involving combinations of ITS, act, ApMat, cal, chs1, gapdh, gs, his3, tub2 gene/markers; eight previously described species (C. aenigma, C. alienum, C. fructicola, C. gloeosporioides sensu stricto, C. karstii, C. nupharicola, C. siamense, C. theobromicola) and a novel species (C. perseae) were identified, as avocado anthracnose pathogens in Israel; and reconfirmed after pathogenicity assays. Colletotrichum perseae sp. nov. and teleomorph of C. aenigma are described along with comprehensive morphological descriptions and illustrations, for the first time in this study.

  4. Stem Bark Extract and Fraction of Persea americana (Mill. Exhibits Bactericidal Activities against Strains of Bacillus cereus Associated with Food Poisoning

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    David A. Akinpelu

    2014-12-01

    Full Text Available The study investigates the in vitro antibacterial potentials of stem bark extracts of Persea americana on strains of Bacillus cereus implicated in food poisoning. The crude stem bark extracts and butanolic fraction at a concentration of 25 mg/mL and 10 mg/mL, respectively, exhibited antibacterial activities against test isolates. The zones of inhibition exhibited by the crude extract and the fraction ranged between 10 mm and 26 mm, while the minimum inhibitory concentration values ranged between 0.78 and 5.00 mg/mL. The minimum bactericidal concentrations ranged between 3.12 mg/mL–12.5 mg/mL and 1.25–10 mg/mL for the extract and the fraction, respectively. The butanolic fraction killed 91.49% of the test isolates at a concentration of 2× MIC after 60 min of contact time, while a 100% killing was achieved after the test bacterial cells were exposed to the butanolic fraction at a concentration of 3× MIC after 90 min contact time. Intracellular protein and potassium ion leaked out of the test bacterial cells when exposed to certain concentrations of the fraction; this is an indication of bacterial cell wall disruptions by the extract’s butanolic fraction and, thus, caused a biocidal effect on the cells, as evident in the killing rate test results.

  5. Avocado (Persea americana Mill.) phenolics, in vitro antioxidant and antimicrobial activities, and inhibition of lipid and protein oxidation in porcine patties.

    Science.gov (United States)

    Rodríguez-Carpena, Javier-Germán; Morcuende, David; Andrade, María-Jesús; Kylli, Petri; Estévez, Mario

    2011-05-25

    The first aim of the present work (study 1) was to analyze ethyl acetate, 70% acetone, and 70% methanol extracts of the peel, pulp, and seed from two avocado (Persea americana Mill.) varieties, namely, 'Hass' and 'Fuerte', for their phenolic composition and their in vitro antioxidant activity using the CUPRAC, DPPH, and ABTS assays. Their antimicrobial potential was also studied. Peels and seeds had higher amounts of phenolics and a more intense in vitro antioxidant potential than the pulp. Peels and seeds were rich in catechins, procyanidins, and hydroxycinnamic acids, whereas the pulp was particularly rich in hydroxybenzoic and hydroxycinnamic acids and procyanidins. The total phenolic content and antioxidant potential of avocado phenolics was affected by the extracting solvent and avocado variety. The avocado materials also displayed moderate antimicrobial effects against Gram-positive bacteria. Taking a step forward (study 2), extracts (70% acetone) from avocado peels and seeds were tested as inhibitors of oxidative reactions in meat patties. Avocado extracts protected meat lipids and proteins against oxidation with the effect on lipids being dependent on the avocado variety.

  6. Removal of Procion Red dye from colored effluents using H2SO4-/HNO3-treated avocado shells (Persea americana) as adsorbent.

    Science.gov (United States)

    Georgin, Jordana; da Silva Marques, Bianca; da Silveira Salla, Julia; Foletto, Edson Luiz; Allasia, Daniel; Dotto, Guilherme Luiz

    2018-03-01

    The treatment of colored effluents containing Procion Red dye (PR) was investigated using H 2 SO 4 and HNO 3 modified avocado shells (Persea americana) as adsorbents. The adsorbent materials (AS-H 2 SO 4 and AS-HNO 3 ) were properly characterized. The adsorption study was carried out considering the effects of adsorbent dosage and pH. Kinetic, equilibrium, and thermodynamic aspects were also evaluated. Finally, the adsorbents were tested to treat simulated dye house effluents. For both materials, the adsorption was favored using 0.300 g L -1 of adsorbent at pH 6.5, where, more than 90% of PR was removed from the solution. General order model was able to explain the adsorption kinetics for both adsorbents. The Sips model was adequate to represent the isotherm data, being the maximum adsorption capacities of 167.0 and 212.6 mg g -1 for AS-H 2 SO 4 and AS-HNO 3 , respectively. The adsorption processes were thermodynamically spontaneous, favorable (- 17.0 Avocado shells, after a simple acid treatment, can be a low-cost option to treat colored effluents.

  7. Antibacterial activity of defensin PaDef from avocado fruit (Persea americana var. drymifolia) expressed in endothelial cells against Escherichia coli and Staphylococcus aureus.

    Science.gov (United States)

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Suárez-Rodríguez, Luis M; Salgado-Garciglia, Rafael; Rodríguez-Zapata, Luis C; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

    2013-01-01

    Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 μg/mL total protein (27-38%) but was more evident at 100 μg/mL (52-65%). This inhibition was higher than the effect showed by polyclonal population (~23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

  8. Determination of changes in the metabolic profile of avocado fruits (Persea americana) by two CE-MS approaches (targeted and non-targeted).

    Science.gov (United States)

    Contreras-Gutiérrez, Paulina K; Hurtado-Fernández, Elena; Gómez-Romero, María; Ignacio Hormaza, José; Carrasco-Pancorbo, Alegría; Fernández-Gutiérrez, Alberto

    2013-10-01

    A CZE method with two different MS detection conditions (MRM and Full Scan) was developed to determine qualitative and quantitative changes in the metabolic profile of avocado fruits (Persea americana). LODs in MRM approach were found between 20.1 and 203.0 ppb for abscisic acid and perseitol, respectively, whilst in Full Scan, varied within the range 0.22–1.90 ppm for the same metabolites. The RSDs for reproducibility test did not exceed 11.45%. The two MS approaches were used to quantify 10 metabolites (phenolic acids, flavonoids, a carbohydrate, an organic acid, a vitamin and a phytohormone) in 18 samples of avocado at different ripening states, and the achieved results were compared. Perseitol, quinic, chlorogenic, trans-cinnamic, pantothenic and abscisic acids, as well as epicatechin and catechin decreased during the ripening process, whereas ferulic and p-coumaric acids showed the opposite trend. Moreover, some other unknown compounds whose concentration changed largely during ripening were also studied by MS/MS and QTOF MS to get a tentative identification.

  9. Thermal stability of oils added with avocado (Persea americana cv. Hass) or olive (Olea europaea cv. Arbequina) leaf extracts during the French potatoes frying.

    Science.gov (United States)

    Jiménez, Paula; García, Paula; Bustamante, Andrés; Barriga, Andrés; Robert, Paz

    2017-04-15

    Effect of the addition of avocado (Persea americana cv. Hass) or olive (Olea europaea cv. Arbequina) hydroalcoholic leaf extracts (AHE and OHE, respectively) on thermal stability of canola oil (CO) and high oleic sunflower oil (HOSO) during French potatoes frying at 180°C was studied. The extracts were characterized by the total phenolic content, phenol chromatographic profiles and antioxidant activity. B-type trimer procyanidins were the major phenolic compounds identified in AHE. OHE showed higher phenol content, antioxidant activity regarding AHE. CO+OHE and HOSO+OHE decreased the formation of polar compounds and showed an anti-polymeric effect with respect to oils without extracts, whereas AHE extract showed a prooxidant effect on HOSO. Therefore, OHE showed an antioxidant effect on HOSO and CO under the studied conditions. In addition, all systems (CO+AHE, HOSO+AHE, CO+OHE and HOSO+OHE) increased the retention of tocopherols. These results demonstrate the potential utility of OHE as natural antioxidant for oils. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. EFFECT OF METHANOLIC SEED EXTRACT OF PERSEA AMERICANA(AVOCADO PEAR ON PROTHROMBIN TIME AND ACTIVATED PARTIAL THROMBOPLASTIN TIME IN MICE

    Directory of Open Access Journals (Sweden)

    2016-11-01

    Full Text Available Twenty (20 adult albino mice were used in the study to determine the effect of methanolic seed extract of Persea Americana on prothrombin time (PT and activated partial thromboplastin time (APTT test. The mice were obtained and kept for 2 weeks to acclimatize. They were weighed and divided into 5 groups. Group A served as control without the extract. Groups B to E were orally administered with graded doses of 200mg, 400 mg, 800 mg and 1600mg/kg body weight per mice daily for 28 days. Blood samples were collected through the median canthus into ti-sodium citrate anticoagulant containers for the analysis of PT and APTT, using standard operative procedure. The analysis was carried out at the Haematology Laboratory of University of Nigeria Teaching Hospital (UNTH Enugu. The results showed a prolonged APTT time at all the doses of the extract when compared with the control (P and lt;0.05. The prothrombin time at the dosage of 200mg/kg did not differ when compared with the control (P and gt;0.05. The increase in PT and APTT was dose dependent. This result pattern suggests that the extract causes prolonged prothrombin time and APTT at various concentrations possibly due to its high potassium content. The extract can be recommended in anticoagulant therapy since it prolongs PT and APTT.

  11. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Jaquelina Julia Guzmán-Rodríguez

    2013-01-01

    Full Text Available Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP represent attractive control agents. Mexican avocado (Persea americana var. drymifolia is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp encoding a protein (78 aa homologous with plant defensins (>80%. We expressed the defensin PaDef cDNA (pBME3 in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%. Also, S. aureus viability was inhibited from 50 μg/mL total protein (27–38% but was more evident at 100 μg/mL (52–65%. This inhibition was higher than the effect showed by polyclonal population (~23%. Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

  12. PaDef defensin from avocado (Persea americana var. drymifolia) is cytotoxic to K562 chronic myeloid leukemia cells through extrinsic apoptosis.

    Science.gov (United States)

    Flores-Alvarez, Luis José; Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Salgado-Garciglia, Rafael; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

    2018-06-01

    Plant defensins, a group of antimicrobial peptides, show selective cytotoxicity toward cancer cells. However, their mechanisms of action remain poorly understood. Here, we evaluated the cytotoxicity of PaDef defensin from avocado (Persea americana var. drymifolia) on K562 chronic myeloid leukemia cells and analyzed the pathway involved in the induction of cell death. The defensin PaDef was not cytotoxic against human PBMCs; however, it was cytotoxic for K562 cell line (IC 50  = 97.3 μg/ml) activating apoptosis at 12 h. PaDef did not affect the mitochondrial membrane potential (ΔΨm), neither the transmembranal potential or the release of intracellular calcium. Also, PaDef induced gene expression of caspase 8 (∼2 fold), TNF-α (∼4 fold) and TNFR1 (∼10 fold). In addition, the activation of caspase 8 was detected at 24 h, whereas caspase 9 activity was not modified, suggesting that the extrinsic apoptosis pathway could be activated. In conclusion, PaDef induces apoptosis on K562 cells, which is related to the activation of caspase 8 and involves the participation of TNF-α, which is a novel property for a plant defensin. Copyright © 2018 Elsevier Ltd. All rights reserved.

  13. The defensin from avocado (Persea americana var. drymifolia) PaDef induces apoptosis in the human breast cancer cell line MCF-7.

    Science.gov (United States)

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Salgado-Garciglia, Rafael; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

    2016-08-01

    Antimicrobial peptides (AMPs) are cytotoxic to cancer cells; however, mainly the effects of AMPs from animals have been evaluated. In this work, we assessed the cytotoxicity of PaDef defensin from avocado (Persea americana var. drymifolia) on the MCF-7 cancer cell line (a breast cancer cell line) and evaluated its mechanism of action. PaDef inhibited the viability of MCF-7 cells in a concentration-dependent manner, with an IC50=141.62μg/ml. The viability of normal peripheral blood mononuclear cells was unaffected by this AMP. Additionally, PaDef induced apoptosis in MCF-7 cells in a time-dependent manner, but did not affect the membrane potential or calcium flow. In addition, PaDef IC50 induced the expression of cytochrome c, Apaf-1, and the caspase 7 and 9 genes. Likewise, this defensin induced the loss of mitochondrial Δψm and increased the phosphorylation of MAPK p38, which may lead to MCF-7 apoptosis by the intrinsic pathway. This is the first report of an avocado defensin inducing intrinsic apoptosis in cancer cells, which suggests that it could be a potential therapeutic molecule in the treatment of cancer. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  14. Changes in the n-alkane composition of avocado pulp oil ( Persea americana, Mill. during fruit ripening

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    Giuffrè, A. M.

    2005-03-01

    Full Text Available The n-alkane composition of Avocado pulp oil (cv. Hass was investigated during fruit ripening. Three samples of fruit were harvested on March 3, 2003, March 18, 2003 and April 2, 2003. Glass gravity column chromatography was employed to separate n-alkanes from other minor components contained in the unsaponifiable fraction. Gas chromatography was used to analyze the eluate. Fourteen compounds were detected ranging from n -C21 to n -C34; mainly n -C24, followed by n -C25 and then by n -C23. Quantities of n -C21, n -C22, n -C23, n -C27 and n -C28 progressively increased during ripening, whereas n -C24, n -C25, n -C26, n -C29, n -C30 and n -C34 decreased from the first harvest date to the third harvest date. While odd-numbered carbon n-alkanes increased (52.38 %, 52.85 % and 53.06 % for the three samples respectively, even-numbered carbon n-alkanes decreased as the fruit ripened (47.62 %, 47.15 % and 46.94 %. The total n-alkane content decreased during ripening, from 25.20 mg/Kg (first harvest date to 16.77 mg/Kg (third harvest date. In order to minimize.Se ha analizado la composición en hidrocarburos lineales saturados del aceite de la pulpa de aguacate (variedad Hass. Tres muestras fueron recolectadas: el 3 de marzo 2003, el 18 de marzo 2003 y el 2 de abril 2003. La separación de los hidrocarburos lineales saturados se realizó mediante fraccionamiento del insaponificable por cromatografía gravimétrica de adsorción en columna y la determinación de los mismos hidrocarburos por cromatografía gaseosa. 14 compuestos fueron detectados del n- C21 al n- C34. El n- C24 fue el mayoritario, seguido del n- C25 y el n- C23. El porcentaje de n- C21, n- C22, n- C23, n- C27 y n- C28, aumentó durante la maduración, mientras que el porcentaje de n- C24, n- C25, n- C26, n- C29, n- C30 y C34 disminuyó desde el 3 de marzo 2003 hasta el 2 de abril 2003. Los hidrocarburos lineales saturados con número impar de átomos de carbono aumentaron (52.38 %, 52

  15. Identifikasi Metabolit Sekunder Ekstrak Etil Asetat Biji Alpukat (Persea americana Mill. dan Uji Toksisitas Terhadap Larva Udang Artemia salina Leach.

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    Nurdia Asdar

    2014-07-01

    Full Text Available Identification of metabolite compound in acetone Extract from Fruits Avocado seed (Percea americana Mill and toxicity test against Artemia salina Leach. Aim of this research is to identify the secondary metabolite compound in aceton extract from fruits Avocado seed (Percea americana Mill and to determine the potential of secondary metabolites from an avocado extract as anticancer. The compound was obtained from Identification process in several stages, namely extraction, fractination, purification and identification. The identification process was color test, TLC, spectroscopy IR. The toxicity test by Brine Shrimp Lethality Test (BSLT to Artemia salina Leach. The compound obtained from this research was yellow-white crystalline needle-shaped, the purity test with TLC analysis showed a stain in three eluen system on the stain test on three eluent systems with Rf proportions as follows; 0,15 from chloroform:ethyl acetat (6:4, 0,33 from methanol:chloroform (1:9, 0,75 for acetone:ethyl acetat, and positively to the reagent Wagner and gave brown precipitate for indicate as alcaloid group. This result is supported by spectroscopy from compound.While toxicity tests showed condensed acetone extracts  and pure compounds are toxic with LC50 value of each is 20.61 mg/mL and 39,81 mg/mL.

  16. Extraction and classification of lipids from seeds of persea ...

    African Journals Online (AJOL)

    Seed lipids of Persea americana and Chrysophyllum albidum were studied. Lipids were extracted with chloroform-methanol, analysed with silicic column chromatography and thin layer chromatography. The total lipid content of Persea americana was 10.8% while that of Chrysophyllum albidum was 7.7%. Fractionation of ...

  17. Nonhost status of commercial Persea americana 'Hass' to Anastrepha ludens, Anastrepha obliqua, Anastrepha serpentina, and Anastrepha striata (Diptera: Tephritidae) in Mexico.

    Science.gov (United States)

    Aluja, Martín; Díaz-Fleischer, Francisco; Arredondo, José

    2004-04-01

    The objective of this study was to determine the host status in Mexico of commercially cultivated and marketed avocado, Persea americana (Mill.), 'Hass' to Anastrepha ludens (Loew), Anastrepha obliqua (Macquart), Anastrepha serpentina (Wiedemann), and Anastrepha striata (Schiner) (Diptera: Tephritidae). Experiments in Michoacán, Mexico, were carried out in six orchards located at three altitudes above sea level during two times (August-October 2001 and April-June 2002). They included choice ('Hass' avocado plus natural host) and no-choice foraging behavior tests on trees under field cages; no-choice, forced infestation trials on caged, fruit-bearing branches in the field, and with individual fruit under laboratory conditions; infestation trials using 'Hass' avocados left unprotected over 1 and 7 d on the ground of orchards; studies to ascertain depth of oviposition and determine egg hatchability; and experiments to determine susceptibility by using time elapsed since removal of fruit from tree as the experimental variable. We trapped adult Anastrepha (n = 7,936) in all orchards and dissected fruit (n = 7,695) from orchards and packing houses (n = 1,620) in search of eggs or larvae. Most (96.7%) A. ludens, A. obliqua, A. striata, and A. serpentina adults were captured in low-elevation orchards. No eggs or larvae were detected in any of the fruit from foraging behavior studies or dissected fruit from orchards or packing houses. Of 5,200 mature, intact fruit on trees in the field forcibly exposed to no-choice female oviposition activity (five females/fruit), we only found four fruit infested by A. ludens but no adults emerged. 'Hass' avocados only became marginally susceptible to attack by A. ludens (but not A. obliqua, A. serpentina, and A. striata) 24 h after being removed from the tree. Fruit placed on the ground in orchards (n = 3,600) were occasionally infested by Neosilba batesi (Curran) (Diptera: Lonchaeidae), a decomposer, but not Anastrepha spp. Based on our

  18. Incidence and growth of Salmonella enterica on the peel and pulp of avocado (Persea americana) and custard apple (Annona squamosa).

    Science.gov (United States)

    Rezende, Ana Carolina B; Crucello, Juliana; Moreira, Rafael C; Silva, Beatriz S; Sant'Ana, Anderson S

    2016-10-17

    The aim of this study was to assess the incidence and to estimate the growth kinetic parameters (maximum growth rate, μ; lag time, λ; and maximum population, κ) of Salmonella on the peel and pulp of avocado (Perseaamericana var. americana) and custard apple (Annona squamosa L.) as affected by temperature (10-30°C). The incidence of Salmonella was assessed on the peel and pulp of the fruits (n=200 of each fruit), separately, totalizing 800 analyses. Only three samples of custard apple pulp were positive for Salmonella enterica and the three isolates recovered belonged to serotype S. Typhimurium. Salmonella was not recovered from avocado and custard apple peels and from avocado pulp. Generally, the substrate (pulp or peel) of growth did not affect μ values of S. enterica (p>0.05). Very similar μ values were found for S. enterica inoculated in custard apple and avocado. S. enterica presented the highest λ in the peel of the fruits. The growth of S. enterica resulted in larger λ in custard apple in comparison to avocado. For example, the λ of S. enterica in the pulp of custard apple and avocado were 47.0±0.78h and 10.0±3.78h, respectively. The lowest values of κ were obtained at the lower storage temperature conditions (10°C). For instance, κ values of 3.7±0.06log CFU/g and 2.9±0.03log CFU/g were obtained from the growth of S. enterica in avocado and custard apple pulps at 10°C (pavocado led to a κ value of 6.5±0.25log CFU/g, while in the peel led to a κ value of 4.6±0.23log CFU/g (pavocado comprises a better substrate than custard apple for the growth of S. enterica. The square root model fitted to the data obtained in this study and to the growth data available in the literature for other tropical low acid fruits indicated high variability in μ and λ of Salmonella. The results obtained in this study show that whole low acid tropical fruits can harbor Salmonella, and that this foodborne pathogen can not only survive but also grow both on the

  19. Effects of Persea americana Mill (Lauraceae) ["Avocado"] ethanolic leaf extract on blood glucose and kidney function in streptozotocin-induced diabetic rats and on kidney cell lines of the proximal (LLCPK1) and distal tubules (MDBK).

    Science.gov (United States)

    Gondwe, M; Kamadyaapa, D R; Tufts, M A; Chuturgoon, A A; Ojewole, J A O; Musabayane, C T

    2008-01-01

    Extracts of Persea americana Mill (Lauraceae) ("Avocado") have been traditionally used to treat hypertension and diabetes mellitus. Accordingly, we studied the hypoglycaemic and renal function effects of P. americana leaf ethanolic extracts (PAE) in STZ-induced diabetic rats. Oral glucose tolerance responses to various doses of PAE were monitored in fasted rats following a glucose load. Rats treated with deionized water or standard hypoglycaemic drugs acted as untreated and treated positive controls, respectively. Acute renal effects of PAE were investigated in anesthetized rats challenged with 0.077 M NaCl after a 3.5-h equilibration for 4 h comprising 1 h control, 1.5 h treatment and 1.5 h recovery periods. PAE was added to the infusate during the treatment period. Hepatic glycogen concentration was measured after 6 weeks of daily treatment with PAE. PAE induced dose-dependent hypoglycaemic responses in STZ-induced diabetic rats while subchronic PAE treatment additionally increased hepatic glycogen concentrations. Acute PAE infusion decreased urine flow and electrolyte excretion rates, whilst subchronic treatment reduced plasma creatinine and urea concentrations. These results indicate not only the basis of the ethnomedicinal use of P. americana leaf extract in diabetes management, but also of need for further studies to identify and evaluate the safety of PAE's bioactive compounds. (c) 2008 Prous Science, S.A.U. or its licensors. All rights reserved.

  20. EFECTO DE NaCl Y CaCl2 EN EL DESARROLLO DE EJES EMBRIONARIOS DE Persea americana Mill CRIOLLO y 'HASS' CULTIVADOS in vitro

    Directory of Open Access Journals (Sweden)

    Leobarda Guadalupe Ramírez-Guerrero

    2010-01-01

    Full Text Available El objetivo de este estudio fue identificar materiales de aguacate tolerantes a salinidad, útiles como portainjertos. Se sembraron in vitro ejes embrionarios de aguacate criollo y 'Hass' en medio Murashige y Skoog al 100 % de su composición nutrimental en condiciones salinas inducidas con NaCl y CaCl2. Se emplearon cinco niveles de cada sal (0.1, 0.3, 0.5, 0.7, 0.9 %, así como la combinación de éstas en proporción 1,1. Se estudió germinación, emergencia, enraizamiento y supervivencia de brotes de aguacate. El criollo superó al 'Hass' con el 21 % de germinación, 36 % de emergencia, con aproximadamente el 50 % en cada una de las variables de crecimiento y el 10 % de supervivencia de plántulas. El desarrollo de los ejes embrionarios fue más limitado en los tratamientos con NaCl en comparación a los establecidos en CaCl2 y NaCl,CaCl2. Con la técnica in vitro se determinó a los 65 días de cultivo que el material criollo fue más tolerante al estrés salino con respecto al 'Hass'.

  1. Short- and long-term modulation of the lutein epoxide and violaxanthin cycles in two species of the Lauraceae: sweet bay laurel (Laurus nobilis L.) and avocado (Persea americana Mill.).

    Science.gov (United States)

    Esteban, R; Jiménez, M S; Morales, D; Jiménez, E T; Hormaetxe, K; Becerril, J M; Osmond, B; García-Plazaola, J I

    2008-05-01

    Short- and long-term responses of the violaxanthin (V) and lutein epoxide (Lx) cycles were studied in two species of Lauraceae: sweet bay laurel (Laurus nobilis L.) and avocado (Persea americana L.). The Lx content exceeded the V content in shade leaves of both species. Both Lx and V were de-epoxidised on illumination, but only V was fully restored by epoxidation in low light. Violaxanthin was preferentially de-epoxidised in low light in L. nobilis. This suggests that Lx accumulates with leaf ageing, partly because its conversion to lutein is limited in shade. After exposure to strong light, shade leaves of avocado readjusted the total pools of alpha- and beta-xanthophyll cycles by de novo synthesis of antheraxanthin, zeaxanthin and lutein. This occurred in parallel with a sustained depression of F(v)/F(m). In Persea indica, a closely related but low Lx species, F(v)/F(m) recovered faster after a similar light treatment, suggesting the involvement of the Lx cycle in sustained energy dissipation. Furthermore, the seasonal correlation between non-reversible Lx and V photoconversions and pre-dawn F(v)/F(m) in sun leaves of sweet bay supported the conclusion that the Lx cycle is involved in a slowly reversible downregulation of photosynthesis analogous to the V cycle.

  2. The use of Gamma radiation of Cobalt-60 to control avocado moth Stenoma catenifer Walsingham, 1912 (Lepidoptera: Elachistidae) and its effects on the quality of the fruit of Persea americana (Miller) (Lauraceae).; Uso da radiacao gama do Cobalto-60, para controlar a broca-do-abacate Stenoma catenifer Walsingham, 1912 (Lepidoptera: Elachistidae) e seus efeitos na qualidade do fruto de Persea americana (Miller) (Lauraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Lilian Karla Figueira da

    2004-07-01

    This work aimed to evaluate the influence of Gamma radiation of Cobalt-60, in the avocado moth Stenoma catenifer Walsingham, 1912 (Lepidoptera: Elachistidae) and its effects on the quality of the fruit Persea americana (Miller) (Lauraceae). For this research, insects were irradiated in ali phases of their life cycle with doses varying from O to 600 Gy and they were maintained at the temperature of 25 +- 2 deg C, humidity of 70 +- 10% and photo phase of 14 h. The species was raised on natural diet, avocado seeds. The cultivar fruits Geada were irradiated with doses that varied from 0 to 150 Gy, maintained for 15 days at room temperature (20 a 35 deg C and humidity of 70 - 80 %) and 30 days at a temperature of 10 deg C, humidity of 40 - 60 %. The chemical-physics and sensorial analyses were carried out. According to the obtained results, it was verified that the lethal dose of gamma radiation to S. catenifer eggs, was of 75 Gy; for caterpillars and pupas was of 300 Gy. The sterile-dose for upcoming adults from irradiated S. catenifer eggs was of 25 Gy; for upcoming adults from irradiated caterpillars, it was of 100 Gy; for adults coming from irradiated pupas was of 150 Gy and for irradiated adults was of 200 Gy. The irradiation in the avocado fruit, maintained at room temperature for 7 days of storage, caused change in the coloration of the fruit (dark spots and yellowish coloration) and more firmness. The sensorial characteristics were kept and the irradiated fruit was the chosen one as favorite for tasting. The irradiated fruits that were kept at 10 deg C, obtained an increase in the storage period, without changing their chemical physics characteristics. The coloration of the fruits was kept, more firmness and a subtle acidity taste increase, being effective in the conservation of the fruits and in the maintenance of their sensorial characteristics. The use of the gamma radiation as treatment quarantine of S. catenifer it was efficient, should be treated them with

  3. Graphene/TiO2 nanocomposite based solid-phase extraction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for lipidomic profiling of avocado (Persea americana Mill.).

    Science.gov (United States)

    Shen, Qing; Yang, Mei; Li, Linqiu; Cheung, Hon-Yeung

    2014-12-10

    Phospholipids possess important physiological, structural and nutritional functions in biological systems. This study described a solid-phase extraction (SPE) method, employing graphene and titanium dioxide (G/TiO2) nanocomposite as sorbent, for the selective isolation and enrichment of phospholipids from avocado (Persea americana Mill.). Based on the principal that the phosphoryl group in the phospholipid can interact with TiO2 via a bridging bidentate mode, an optimum condition was established for SPE, and was successfully applied to prepare avocado samples. The extracts were monitored by matrix-assisted laser desorption ionization time-of-flight/tandem mass spectrometry (MALDI-TOF/MS) in both positive-ion and negative-ion modes. Results showed that phospholipids could be efficiently extracted in a clean manner by G/TiO2 based SPE. In addition, the signals of phospholipids were enhanced while the noise was reduced. Some minor peaks became more obvious. In conclusion, the nanocomposite material of G/TiO2 was proved to be a promising sorbent for selective separation of phospholipids from crude lipid extract. Copyright © 2014 The Authors. Published by Elsevier B.V. All rights reserved.

  4. Neocosmospora perseae sp. nov., causing trunk cankers on avocado in Italy

    NARCIS (Netherlands)

    Guarnaccia, Vladimiro; Sandoval-Denis, Marcelo; Aiello, Dalia; Polizzi, Giancarlo; Crous, P.W.

    2018-01-01

    Trunk and branch cankers are among the most important diseases compromising avocado production worldwide. A novel species, Neocosmospora perseae sp. nov. is described isolated from trunk lesions on Persea americana in the main avocado producing area of Sicily, Italy. The new species is characterised

  5. Biochemical analysis of reactive oxygen species production and antioxidative responses in unripe avocado (Persea americana Mill var Hass) fruits in response to wounding.

    Science.gov (United States)

    Castro-Mercado, E; Martinez-Diaz, Y; Roman-Tehandon, N; Garcia-Pineda, E

    2009-03-01

    We analyzed the production of reactive oxygen species (ROS) and of detoxifying enzymes and enzymes of the ascorbate (ASC) acid cycle in avocado fruit (Pesea Americana Mill cv Hass) in response to wounding. The levels of superoxide anion (O(2-), hydroxyl radicals (OH.) and hydrogen peroxide (H(2)O(2)) increased at 15 min and 2 and 15 h post-wounding. Peroxidase (POD) activity had increased to high levels 24 h after wounding; in contrast, catalase and superoxide dismutase (SOD) levels hat decreased significantly at 24 h post-treatment. Basic POD was the major POD form induced, and the levels of at least three apoplastic POD isozymes -increased following wounding. Using specific inhibitors, we characterized one MnSOD and two CuZnSOD isozymes. CuZnSOD activities decreased notably 12 h after treatment. The activities of dehydroascorbate reductase and glutathione reductase increased dramatically following the wounding treatment, possibly as a means to compensate for the redox changes due to ROS production.

  6. Description of morphologic characters in avocado trees (Persea americana. Mill) var. Hass, submitted to gamma radiation of {sup 60} Co; Descripcion de caracteres morfologicos en arboles de aguacate (Persea americana. Mill) var. Hass, sometidos a radiacion gama de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Sandoval R, L

    2004-07-01

    It was carried out the characterization of 77 trees of avocado ''Hass'' subjected to gamma radiation of Co{sup 60}, in doses of 0, 5, 10,15, 20 and 25 Gy based on previous studies of radiosensitivity with ends of improvement located in the experimental center ''La Labor'' belonging to the foundation Salvador Sanchez Colin. CICTAMEX. S C. in Temascaltepec, Mexico. It was carried out the morphological characterization of this variety according to the nomenclature settled down by the International Office of Genetic Resources (IBPGRI), registering 27 variables for the description of the tree, 33 for description of the fruit and 14 for the characterization of the seed. Regarding the architectural characterization 10 variables were evaluated. Using gamma radiation of Co{sup 60} in the doses of 15 and 20 Gy it was detected a reduction of 22% in the portage of the tree, likewise had reduction the diameter of the trunk and of the foliage. It was found that the reduction in the behavior of the tree induces to a bigger variation of the form of the same one, in the distribution of the branches in the form of the fruit and in the form of the seed you Also detects a remarkable increment in the productivity of some hoist irradiated regarding the architecture of the tree one observes that the angle of inclination of the branches increase when being increased the radiation dose and that the longitude of the annual section of growth decreased significantly in the doses of 20 and 25 Gy. One observes a significant Increment in the I number of buds prolepticos in those hoist irradiated in the I finish mbdulo of rhythmic growth. Io that indicates a change substantial in the I inhabit of growth With the purpose of determining if the changes in morphology and architecture of the tree corresponded with the modification at level meristematico, they were carried out observations to the court microscope of having knitted apical vegetative meristematico in samples of you hoist of all the treatments including the witness. without detecting significant modifications in disposition and tamario of the cells of the tunic and the body of the bud veqetativo.

  7. USE OF SOIL MICROORGANISMS AS A BIOTECHNOLOGICAL STRATEGY TO ENHANCE AVOCADO (Persea americana)-PLANT PHOSPHATE UPTAKE AND GROWTH USO DE MICROORGANISMOS DEL SUELO COMO ESTRATEGIA BIOTECNOLÓGICA PARA MEJORAR LA ABSORCIÓN DE FÓSFORO Y EL CRECIMIENTO DE PLANTAS DE AGUACATE (Persea americana)

    OpenAIRE

    Nelson Walter Osorio Vega; Sandra Luz Serna Gómez; Beatriz Elena Montoya Restrepo

    2012-01-01

    Abstract. In recent years the area cultivated with avocado has increased significantly in Colombia and this trend is expected to continue in the coming years. However, most of the soils in the highlands of the Colombian Andean, that have been used to grow avocado, are strongly acidic and nutrient deficient, particularly phosphorus (P). To alleviate these problems, high rates of lime and P fertilizers are frequently applied, in many cases the applied dose are excessive. This increases producti...

  8. Description of morphologic characters in avocado trees (Persea americana. Mill) var. Hass, submitted to gamma radiation of {sup 60} Co; Descripcion de caracteres morfologicos en arboles de aguacate (Persea americana. Mill) var. Hass, sometidos a radiacion gama de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Sandoval R, L

    2004-07-01

    It was carried out the characterization of 77 trees of avocado ''Hass'' subjected to gamma radiation of Co{sup 60}, in doses of 0, 5, 10,15, 20 and 25 Gy based on previous studies of radiosensitivity with ends of improvement located in the experimental center ''La Labor'' belonging to the foundation Salvador Sanchez Colin. CICTAMEX. S C. in Temascaltepec, Mexico. It was carried out the morphological characterization of this variety according to the nomenclature settled down by the International Office of Genetic Resources (IBPGRI), registering 27 variables for the description of the tree, 33 for description of the fruit and 14 for the characterization of the seed. Regarding the architectural characterization 10 variables were evaluated. Using gamma radiation of Co{sup 60} in the doses of 15 and 20 Gy it was detected a reduction of 22% in the portage of the tree, likewise had reduction the diameter of the trunk and of the foliage. It was found that the reduction in the behavior of the tree induces to a bigger variation of the form of the same one, in the distribution of the branches in the form of the fruit and in the form of the seed you Also detects a remarkable increment in the productivity of some hoist irradiated regarding the architecture of the tree one observes that the angle of inclination of the branches increase when being increased the radiation dose and that the longitude of the annual section of growth decreased significantly in the doses of 20 and 25 Gy. One observes a significant Increment in the I number of buds prolepticos in those hoist irradiated in the I finish mbdulo of rhythmic growth. Io that indicates a change substantial in the I inhabit of growth With the purpose of determining if the changes in morphology and architecture of the tree corresponded with the modification at level meristematico, they were carried out observations to the court microscope of having knitted apical vegetative meristematico in samples of you hoist of all the treatments including the witness. without detecting significant modifications in disposition and tamario of the cells of the tunic and the body of the bud veqetativo.

  9. COMPOSIÇÃO DE ÁCIDOS GRAXOS DO ÓLEO DO MESOCARPO E DA SEMENTE DE CULTIVARES DE ABACATE (PERSEA AMERICANA, MILL. DA REGIÃO DE RIBEIRÃO PRETO, SP

    Directory of Open Access Journals (Sweden)

    Gisele Lucia MASSAFERA

    2010-11-01

    Full Text Available

    O objetivo deste trabalho foi identificar e determinar os ácidos graxos dos óleos do mesocarpo e da semente de abacates da região de Ribeirão Preto – SP. Para realização do trabalho foram utilizados frutos de abacateiros (Persea americana, Mill. cultivares Fortuna, Ouro Verde e Princesa. O óleo foi obtido através de extração com solvente a quente (Soxhlet e após a esterificação, as amostras foram injetadas em cromatógrafo a gás. No óleo do mesocarpo o ácido graxo que apresentou maior concentração foi o ácido oléico, com teores entre 31,77 e 50,30%, seguido pelo ácido palmítico, com teores entre 20,73 e 32,54%. Os teores de ácidos graxos do óleo do mesocarpo mostraram-se diferentes da semente e variaram entre os cultivares. No óleo do mesocarpo o ácido graxo que apresentou maior concentração foi o ácido oléico, com teores entre 31,77 e 50,30%, seguido pelo ácido palmítico, com teores entre 20,73 e 32,54%. No óleo da semente, o ácido graxo que apresentou maior concentração foi o ácido oléico com teores entre 11,69 e 35,83%, seguido pelo ácido linoléico cujos teores variaram entre 23,28 e 29,98%. No presente estudo pudemos concluir que há uma variabilidade nas concentrações de ácidos graxos entre os diferentes cultivares e também entre a semente e o mesocarpo de cada cultivar.

  10. The use of Gamma radiation of Cobalt-60 to control avocado moth Stenoma catenifer Walsingham, 1912 (Lepidoptera: Elachistidae) and its effects on the quality of the fruit of Persea americana (Miller) (Lauraceae)

    International Nuclear Information System (INIS)

    Silva, Lilian Karla Figueira da

    2004-01-01

    This work aimed to evaluate the influence of Gamma radiation of Cobalt-60, in the avocado moth Stenoma catenifer Walsingham, 1912 (Lepidoptera: Elachistidae) and its effects on the quality of the fruit Persea americana (Miller) (Lauraceae). For this research, insects were irradiated in ali phases of their life cycle with doses varying from O to 600 Gy and they were maintained at the temperature of 25 +- 2 deg C, humidity of 70 +- 10% and photo phase of 14 h. The species was raised on natural diet, avocado seeds. The cultivar fruits Geada were irradiated with doses that varied from 0 to 150 Gy, maintained for 15 days at room temperature (20 a 35 deg C and humidity of 70 - 80 %) and 30 days at a temperature of 10 deg C, humidity of 40 - 60 %. The chemical-physics and sensorial analyses were carried out. According to the obtained results, it was verified that the lethal dose of gamma radiation to S. catenifer eggs, was of 75 Gy; for caterpillars and pupas was of 300 Gy. The sterile-dose for upcoming adults from irradiated S. catenifer eggs was of 25 Gy; for upcoming adults from irradiated caterpillars, it was of 100 Gy; for adults coming from irradiated pupas was of 150 Gy and for irradiated adults was of 200 Gy. The irradiation in the avocado fruit, maintained at room temperature for 7 days of storage, caused change in the coloration of the fruit (dark spots and yellowish coloration) and more firmness. The sensorial characteristics were kept and the irradiated fruit was the chosen one as favorite for tasting. The irradiated fruits that were kept at 10 deg C, obtained an increase in the storage period, without changing their chemical physics characteristics. The coloration of the fruits was kept, more firmness and a subtle acidity taste increase, being effective in the conservation of the fruits and in the maintenance of their sensorial characteristics. The use of the gamma radiation as treatment quarantine of S. catenifer it was efficient, should be treated them with

  11. Aceite de frutos de Persea schiedeana en sobremaduración y cosechados de árboles en estado silvestre

    OpenAIRE

    Campos-Hernández, N; Cruz-Castillo, JG; Hernández-Montes, A; Rubio-Hernández, D

    2011-01-01

    En frutos sobremaduros de Persea schiedeana Nees colectados en árboles creciendo en estado silvestre se estudiaron las características químicas y sensoriales del aceite. La extracción de aceite con isopropanol y hexano tuvo un rendimiento de 12% aceite/pulpa (v/p). El aceite de P. schiedeana fue comparado con aceites comerciales de oliva y aguacate. El aceite de P. schiedeana tuvo menor número de insaturaciones y más ácidos grasos de cadena larga que los aceite de oliva y de aguacate. Los índ...

  12. Changes in proximate and phytochemical compositions of Persea ...

    African Journals Online (AJOL)

    Persea americana (avocado pear leaves, fruits, and seeds) is one of the medicinal herbs that has been widely utilized in treating/managing disease conditions. In this study, we investigated the changes in proximate and phytochemical compositions of avocado seeds associated with ripening using standard methods.

  13. 2-Alquil-4-Hidroxi-Tetrahidrofuranos de la Pulpa del Aguacate

    Directory of Open Access Journals (Sweden)

    Alfonso Rivas-Gil

    2006-12-01

    Full Text Available From a hexane extract of avocado pulp (Persea americana Mill., an 1:3 mixture of 2-(heptadec-16-enyl- and 2-(heptadec-16-ynyl-4-hydroxy -tetrahydrofurans was characterized by NMR techniques. These compounds have not been previously reported from natural sources.

  14. (Persea americana) and African pear (Dacryodes edulis)

    African Journals Online (AJOL)

    GREGO

    2007-04-02

    Apr 2, 2007 ... The percent oil content in the fruit pulp was determined. The oil content of avocado pear .... isomerisation processes which usually lead to loss of unsaturation. The low ... The role of fats and oils in cosmetics. JAOCS 49(71):.

  15. Pollination in avocado flowers (Persea Americana Mill.)

    OpenAIRE

    Malerbo-Souza, Darclet Teresinha; Faculdade de Agronomia Dr. Francisco Maeda; Toledo, Vagner de Alencar Arnaut de; UEM; Silva, Simone Rodrigues da; Faculdade de Agronomia Dr. Francisco Maeda; Sousa, Francisco Fábio; Faculdade de Agronomia Dr. Francisco Maeda

    2008-01-01

    The experiment aimed to study the frequency, nectar and/or pollen and hoarding time of bees in avocado flowers and verify the effect of their visits on fruit production. Six inflorescences (three covered and three uncovered) with two replications were marked to evaluate the effect of cross pollination on fruition percentage. The honey bees showed two peaks of hoarding (by 11 to 12 a.m. and 5 p.m.) following the flowers opening of different avocado groups (groups A and B), as much for nectar a...

  16. Persea schiedeana : A High Oil “Cinderella Species” Fruit with Potential for Tropical Agroforestry Systems

    OpenAIRE

    Jay Bost

    2013-01-01

    Persea schiedeana , a close relative of avocado ( Persea americana ), is an important part of agroforestry systems and diets in parts of Mesoamerica, particularly in the coffee growing areas of southeastern Mexico and Guatemala, where it is known as chinene , coyo , and yas . Little research attention has been given to this species, other than as a rootstock for avocado. Research carried out in six villages composing the Comité de Recursos Naturales de la Chinantla Alta (CORENCHI) in Oaxaca, ...

  17. LA VARIABILIDAD GENÉTICA DE LA Persea americana M.: UN ESTUDIO SOBRE LA ENSEÑANZA DE LA BIOLOGÍA MOLECULAR DESDE EL LABORATORIO EN GRADO 10°

    Directory of Open Access Journals (Sweden)

    Carlos Miguel Monsalve Agudelo

    2015-12-01

    Full Text Available El presente artículo, expone el trabajo realizado en desarrollo de proyecto de grado de maestría, con estudiantes de la I.E. San Fernando de Amagá, Antioquia, en el periodo comprendido entre Julio de 2013 y junio de 2014, en la línea de profundización de la enseñanza de la biología molecular. Para la ejecución del trabajo se tomaron como base dos grupos, uno control y uno experimental, en los cuales se aplicó un ciclo de aprendizaje a través de videos sobre variabilidad genética explicada en la expedición de Darwin, situaciones problema, clases magistrales y trabajos de campo como la diferenciación de especies vegetales a partir de características morfológicas. En el grupo experimental se introdujo el trabajo de laboratorio como base de la enseñanza de la variabilidad genética desde la biología molecular. Desde el punto de vista pedagógico, se considera que la intervención permitió un avance en el proceso de aprendizaje de los estudiantes en el acercamiento a los contenidos conceptuales, procedimentales y actitudinales referentes a la variabilidad genética. Así mismo desde el aspecto biológico, se determinó la variabilidad genética del aguacate en el municipio de Amagá, al resultar diferente dos de las muestras en la prueba del gel electroforesis PCR-RFLPs para 6 muestras de plantas de Aguacate con marcador de 50pb.

  18. Impacto de estrategias de mercado para la exportación de palta orgánica (Persea americana), de la Región Junín a Estados Unidos

    OpenAIRE

    Nuñez Rojas, Wilfredo Edison

    2016-01-01

    Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Agronegocios La exportación de palta peruana está en crecimiento, con una ventaja comparativa y una ventaja competitiva en cuanto a calidad y costos de producción, en toda la sierra peruana las áreas de producción están en crecimiento debido a ello los productores requieren de estrategias de mercado para el comercio internacional. Objetivos. Analizar las estrategias de mercado para la exportación de la palta (Persea a...

  19. Determinação enzimática de dopamina em formulações farmacêuticas utilizando sistema de análise por injeção em fluxo com extrato bruto de abacate (Persea americana

    Directory of Open Access Journals (Sweden)

    Lupetti Karina Omuro

    2003-01-01

    Full Text Available In this work, a spectrophotometric flow injection analysis system using a crude extract of avocado (Persea americana as a source of polyphenol oxidase to dopamine determination was developed. The substrates and enzyme concentrations from 2.4x10-7 to 5.3x10-4 mol L-1 and 28 to 332 units mL-1 were evaluated, respectively. In addition, the FIA parameters such as sample loop (50 to 500 mL, flow rate (1.4 to 4.3 mL min-1 and reactor length (100 to 500 cm were also evaluated in a 0.1 mol L-1 phosphate buffer solution (pH 7.0. Dopamine solution concentrations were determined using 277 units mL-1 enzyme solution, 400 mL enzyme loop, 375 mL sample loop, 2.2 mL min-1 flow rate and a reactor of 350 cm. The analytical curve showed a linearity from 5.3x10-5 to 5.3x10-4 mol L-1 dopamine with a detection limit of 1.3x10-5 mol L-1. The analytical frequency was 46 h-1 and the RSD lower than 0.5% for 5.3x10-4 mol L-1 dopamine solution (n=10. A paired t-test showed that all results obtained for dopamine in commercial formulations using the proposed flow injection procedure and a spectrophotometric procedure agree at the 95% confidence level.

  20. Las plantas americanas en la obra de Charles de l'Écluse: primeras citas en las cartas de Juan de Castañeda

    Directory of Open Access Journals (Sweden)

    Ramón-Laca Menéndez de Luarca, Luis

    1999-06-01

    Full Text Available The Flemish botanist Charles de l'Écluse's works contain abundant references to American plants, for example the avocado (Persea americana Mill., the sweet potato [Ipomoea batatas (L. Lam.], the potato (Solanum tuberosum L. and the tobáceo (Nicotiana tabacum L.. Although some of these plants were observed by L'Écluse himself during his expedition to the Iberian Península between 1564 and 1565, the majority of the American plants quoted in his works are related with shipments received from several agents around Europe. In the case of Spain, L'Écluse obtained seeds from Seville thanks to the help of Simón de Tovar and Juan de Castañeda. The latter offers in his letters important innovations: Annona cherimola Mill., Indigofera añil LJI. tinctoria L., Annona muricata L., Mammea americana L.. Annona reticulata L., Dioscorea sp., Aloysia citrodora Palau or Manihot spj M. esculenta Crantz. In most of the cases the mention of these plants represente the first record of cultivation in the Old World. A percentage of this material was used by L'Écluse in his Exoticorum libri decem, published in Antwerp in l605.Las obras del botánico flamenco Charles de l'Écluse contienen infinidad de referencias a plantas americanas, destacando entre ellas el aguacate (Persea americana Mill., las batatas [Ipomoea batatas (L. Lam.], las patatas (Solanum tuberosum L. o el tabaco (Nicotiana tabacum L. . Algunas de ellas fueron observadas por L'Écluse durante la expedición llevada a cabo por la Península Ibérica entre 1564 y 1565. La mayor parte de plantas americanas citadas en sus obras están relacionadas sin embargo con envíos realizados por diversos corresponsales repartidos por Europa. En el caso de España, L'Écluse recibió semillas desde Sevilla gracias a Simón de Tovar y Juan de Castañeda. Este último ofrece en sus cartas importantes novedades, como el "anón" (Annona cherimola Mill., el "añil" (Indigofera añil L./I. tinctoria L., la "guan

  1. Biology and systematics of the New World Phyllocnistis Zeller leafminers of the avocado genus Persea (Lepidoptera, Gracillariidae

    Directory of Open Access Journals (Sweden)

    Donald Davis

    2011-05-01

    Full Text Available Four New World species of Phyllocnistis Zeller are described from serpentine mines in Persea (Family Lauraceae. Phyllocnistis hyperpersea, new species, mines the upper leaf surfaces of avocado, Persea americana Mill., and red bay, Persea borbonia (L. Spreng. and ranges over much of the southeastern United States into Central America. Phyllocnistis subpersea, new species, mines the underside and occasionally upper sides of new leaves of Persea borbonia in southeastern United States. Phyllocnistis longipalpa, new species, known only from southern Florida also mines the undersides of new leaves of Persea borbonia. Phyllocnistis perseafolia, new species, mines both leaf surfaces and possibly fruits of Persea americana in Colombia, South America. As in all known species of Phyllocnistis, the early instars are subepidermal sapfeeders in young (not fully hardened foliage, and the final instar is an extremely specialized, nonfeeding larval form, whose primary function is to spin the silken cocoon, at the mine terminus, prior to pupation. Early stages are illustrated and described for three of the species. The unusual morphology of the pupae, particularly the frontal process of the head, is shown to be one of the most useful morphological sources of diagnostic characters for species identification of Phyllocnistis. COI barcode sequence distances are provided for the four proposed species and a fifth, undescribed species from Costa Rica.

  2. Biology and systematics of the New World Phyllocnistis Zeller leafminers of the avocado genus Persea (Lepidoptera, Gracillariidae).

    Science.gov (United States)

    Davis, Donald R; Wagner, David L

    2011-05-11

    Four New World species of Phyllocnistis Zeller are described from serpentine mines in Persea (Family Lauraceae). Phyllocnistis hyperpersea,new species, mines the upper leaf surfaces of avocado, Persea americana Mill., and red bay, Persea borbonia (L.) Spreng. and ranges over much of the southeastern United States into Central America. Phyllocnistis subpersea,new species, mines the underside and occasionally upper sides of new leaves of Persea borbonia in southeastern United States. Phyllocnistis longipalpa, new species, known only from southern Florida also mines the undersides of new leaves of Persea borbonia. Phyllocnistis perseafolia,new species, mines both leaf surfaces and possibly fruits of Persea americana in Colombia, South America. As in all known species of Phyllocnistis, the early instars are subepidermal sapfeeders in young (not fully hardened) foliage, and the final instar is an extremely specialized, nonfeeding larval form, whose primary function is to spin the silken cocoon, at the mine terminus, prior to pupation. Early stages are illustrated and described for three of the species. The unusual morphology of the pupae, particularly the frontal process of the head, is shown to be one of the most useful morphological sources of diagnostic characters for species identification of Phyllocnistis. COI barcode sequence distances are provided for the four proposed species and a fifth, undescribed species from Costa Rica.

  3. Hasat Zamanı ve Hasat Sonrası Olgunluğa Bağlı Olarak Bazı Avokado (Persea americana Mill Çeşitlerinin Bileşimindeki Değişimler

    Directory of Open Access Journals (Sweden)

    Feramuz Özdemir

    2015-02-01

    Full Text Available Bu araştırmada Bacon, Fuerte, Hass ve Zutano çeşidi avokado (Persea americana Mill meyvelerinin bazı fiziksel ve kimyasal özelliklerinin hasat zamanı ve hasat sonrası olgunlaşma periyoduna göre değişimi belirlenmiştir. Ortalama meyve eti oranı %81.3 olan örneklerde toplam kuru madde %21.88-26.25, yağ %12.22-17.28, protein %1.63-2.42, kül %0.94-1.27 ve titre edilebilir asit miktarı %0.08-0.11 (sitrik asit değerleri arasında değişmiştir. Avokado yağında en fazla oleik asit {%49.66-66.51 bulunurken, bu yağ asilini, palmitik asit (%15.35-22.26 ve linoleik asit (%9.88-15.60 izlemiştir. Meyvede mineral maddelerden potasyum 4396-5882 mg/kg, magnezyum 179.8-259.1 mg/kg, kalsiyum 73.49-99.70 mg/kg, sodyum 16.64-27.48 mg/kg, demir 1.88-6.07 mg/kg, bakır 2.24-3.42 mg/kg, çinko 3.09-4.95 mg/kg ve mangan 0.73-1.21 mg/kg değerleri arasında değişim göstermiştir. Avokado hasatının geciktirilmesi ile meyve ağırlığı, toplam kuru madde, yağ ve protein miktarının arttığı saptanmıştır. Aynı zamanda örneklerin yağ asidi kompozisyonunun hasat zamanına göre değiştiği, geç hasatla birlikte oleik asit oranı artarken, diğer yağ asitlerinin oranı ya azalmış ya da değişmemiştir. Hasat sonrası olgunlaşma döneminde ise yağ asitleri bakımından doymamışlık oranının arttığı saptanmıştır.

  4. Enfermedades del Aguacate en La Florida

    Directory of Open Access Journals (Sweden)

    Stevens H. E.

    1942-04-01

    Full Text Available El aguacate cultivado bajo las condiciones ambienciales de La Florida, está sujeto a ser atacado por varias enfermedades parasitarias. Algunas de estas son de menor importancia y no requieren métodos de represión especiales; otras al contrario son más severas y requieren atención cada año; y otras además están sujetas a las condiciones climatológicas, y su intensidad dependerá principalmente de las condiciones meteorológicas durante algunos períodos críticos en el crecimiento del árbol.

  5. Characterization of avocado pear ( Persea americana ) and African ...

    African Journals Online (AJOL)

    Dacryodes edulis) were extracted with chloroform. The oil were characterized for melting point, refractive index, relative viscosity, free fatty acids, saponification value, iodine value, acid value and percentage unsaponifable matter. The percent oil ...

  6. Regeneration from irradiated avocado (Persea americana Mill.) embryogenic cultures

    Energy Technology Data Exchange (ETDEWEB)

    Witjaksono, Y.; Nugraheni, U. K.; Hoesen, D. H. [Plant Cell and Tissue Culture Laboratory, Bogor Botanic Garden, Bogor (Indonesia); Litz, R. E. [Tropical Research and Education Center, University of Florida, Homestead, FL (United States)

    2009-05-15

    Somatic embryogenesis was induced from zygotic embryos excised from immature avocado fruit from selected genotypes grown in the highlands of Cisarua, West Bogor, Indonesia. The proembryonic masses developed first on semi-solid medium and were then transferred to liquid cultures for proliferation. The embryogenic masses were then irradiated at 9, 18 and 35 Gy using a {sup 60}Co irradiation source. 3 sub-cultures in liquid medium ensured adequate proliferation prior to transfer to fresh development medium. After 1-3 months, somatic embryos with more than 0.5 cm in diameter were transferred to a germination medium, while the smaller somatic embryos (<0.5 cm in diameter) were sub-cultured one more time for additional growth. After 1-2 months on germination medium, plantlets were transferred individually to new medium. (author)

  7. Ovary starch reserves and pistil development in avocado (Persea americana).

    Science.gov (United States)

    Alcaraz, M Librada; Hormaza, J Ignacio; Rodrigo, Javier

    2010-12-01

    In avocado, only a very small fraction of the flowers are able to set fruit. Previous work in other woody perennial plant species has shown the importance of carbohydrates accumulated in the flower in the reproductive process. Thus, in order to explore the implications of the nutritive status of the flower in the reproductive process in avocado, the starch content in the pistil has been examined in individual pollinated and non-pollinated flowers at anthesis and during the days following anthesis. Starch content in different pistilar tissues in each flower was quantified with the help of an image analysis system attached to a microscope. Flowers at anthesis were rich in highly compartmentalized starch. Although no external morphological differences could be observed among flowers, the starch content varied widely at flower opening. Starch content in the ovary is largely independent of flower size because these differences were not correlated with ovary size. Differences in the progress of starch accumulation within the ovule integuments between pollinated and non-pollinated flowers occurred concomitantly with the triggering of the progamic phase. The results suggest that starch reserves in the ovary could play a significant role in the reproductive process in avocado. Copyright © Physiologia Plantarum 2010.

  8. Pemanfaatan Biji Alpukat (Persea Americana Mill.) Untuk Pembuatan Edible Film

    OpenAIRE

    Yudiandani, Ana '; Efendi, Raswen '; Ibrahim, Ahmad '

    2016-01-01

    Avocado seed was found to have high starchs of content and yet it has not been optimally utilized. Therefore this research was aimed to utilized the starchs of avocado seed as material of edible film and to get the best formulations of addition of starchs avocado seed. This research was conducted experimentaly by used Complete Randomized Design (CRD) with five treatmens and three replications which followed by Duncan's New Multiple Range Test (DNMRT) at level 5%. The treatmens in this researc...

  9. Supply of avocado starch (Persea americana mill) as bioplastic material

    Science.gov (United States)

    Ginting, M. H. S.; Hasibuan, R.; Lubis, M.; Alanjani, F.; Winoto, F. A.; Siregar, R. C.

    2018-02-01

    The purpose of this study was to determine the effect of time precipitation of avocado slurry seed to yield of starch. Starch analysis included starch content, moisture content, amylose content, amylopectin content, ash content, protein content, fat content, Fourier transform infra red analysis and rapid visco analyzer. Supply of starch from avocado seeds was used by extraction method. Every one hundred grams of avocado slurry was precipitated by gravity with variations for 4 hours, 8 hours, 12 hours, 16 hours, 20 hours and 24 hours. The Starch yield was washed, and dried using oven at 70°C for 30 minutes. Starch yield was the highest as 24.20 gram at 24 hours. The result of starch characterization was 73.62%, water content 16.6%, amylose 0.07%, amylopectin 73.55%, ash content 0.23%, protein content 2.16%, fat content 1.09%. Rapid visco analyzer obtained at 91.33°C of gelatinization temperature. Scanning electron microscopy analyzes obtained 20 μm oval-shaped starch granules. Fourier Transform Infra Red analysis of starch obtained the peak spectrum of O-H group of alcohols, C-H alkanes and C-O ether.

  10. A model biorefinery for avocado (Persea americana mill.) processing.

    Science.gov (United States)

    Dávila, Javier A; Rosenberg, Moshe; Castro, Eulogio; Cardona, Carlos A

    2017-11-01

    This research investigated and evaluated a biorefinery for processing avocado Hass variety into microencapsulated phenolic compounds extract, ethanol, oil and xylitol. Avocado was first characterized for its potential valuable compounds; then, the techno-economic and environmental aspects of the biorefinery were developed and finally the total production costs and potential environmental impact of the proposed biorefinery were investigated. Four scenarios of the biorefinery were evaluated with different extent of mass and energy integration as well as the incorporation of a cogeneration system. Results indicated that the main fatty acid in the pulp of the investigated avocado variety was oleic acid (50.96%) and that this fruit contained significant amount of holocellulose (52.88% and 54.36% in the peel and seed, respectively). Techno-economic and environmental assessment suggested an attractive opportunity for a biorefinery for complete utilization of the avocado fruit as well the importance of the level of integration. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Optimization of controlled pollination in avocado (persea americana Mill., Lauraceae)

    OpenAIRE

    Alcaraz Arco, María Librada; Hormaza Urroz, José Ignacio

    2014-01-01

    Avocado has a singular synchronous protogynous dichogamy breeding system that promotes outcrossing. In this work different steps have been optimized to improve controlled pollinations in avocado in order to perform basic studies of reproductive biology and directed crosses in breeding programs. The results show that, in order to achieve successful fruit set, male flowers should be collected when all the anthers have dehisced and the pollen transferred by direct contact of the anthers with the...

  12. Descripción de la hembra de Copaxa ignescens (Lepidoptera: Saturniidae, con anotaciones sobre sus primeros estadios inmaduros

    Directory of Open Access Journals (Sweden)

    Amarillo S. Angela R.

    1997-06-01

    Full Text Available The female of Copaxa ignescens Lemaire, 1978 (Saturniidae is described and notes on the first three larval instars are presented. The caterpillars were reared on Avocado (Persea americana Miller.Se describe la hembra de Copaxa ignescens Lemaire, 1978 (Saturniidae y se hacen anotaciones sobre los tres primeros estadios larvales. Las orugas se criaron con Aguacate (Persaa americana Miller.

  13. Persea schiedeana: A High Oil “Cinderella Species” Fruit with Potential for Tropical Agroforestry Systems

    Directory of Open Access Journals (Sweden)

    Jay Bost

    2013-12-01

    Full Text Available Persea schiedeana, a close relative of avocado (Persea americana, is an important part of agroforestry systems and diets in parts of Mesoamerica, particularly in the coffee growing areas of southeastern Mexico and Guatemala, where it is known as chinene, coyo, and yas. Little research attention has been given to this species, other than as a rootstock for avocado. Research carried out in six villages composing the Comité de Recursos Naturales de la Chinantla Alta (CORENCHI in Oaxaca, Mexico shows that Persea schiedeana has potential as a supplement to avocado production in subsistence systems and as a potential oil crop in more market oriented agroforestry systems. This survey of Persea schiedeana in the Chinantla area reports on the ethnoecology and management of chinene, as well as on the morphological diversity of the fruit in the area. High morphological diversity for fruit characters was noted and it is suggested that artificial selection has occurred and been modestly successful for desired fruit characters. Superior fruiting trees, identified during village level “chinene fairs” were targeted for vegetative propagation as part of a participatory domestication project. Such superior genotypes hold potential for addressing food security and creating marketable products in tropical areas around the globe.

  14. La competitividad del aguacate mexicano en el mercado estadounidense

    OpenAIRE

    Torres Preciado, Víctor Hugo

    2009-01-01

    Resumen El objetivo de esta investigación consiste en analizar la competitividad de las exportaciones del aguacate mexicano hacia Estados Unidos. Para tal fin, se aplica el Índice de Ventaja Relativa de Exportación (VRE) y el método de Participación Constante de Mercado (CMS). Los resultados sugieren que México es altamente competitivo en las exportaciones de aguacate y que esta tendencia parece sostenerse en los próximos años. Abstract This research aims to analyze the competitiv...

  15. La competitividad del aguacate mexicano en el mercado estadounidense

    OpenAIRE

    Torres Preciado, Víctor Hugo

    2009-01-01

    Resumen El objetivo de esta investigación consiste en analizar la competitividad de las exportaciones del aguacate mexicano hacia Estados Unidos. Para tal fin, se aplica el Índice de Ventaja Relativa de Exportación (VRE) y el método de Participación Constante de Mercado (CMS). Los resultados sugieren que México es altamente competitivo en las exportaciones de aguacate y que esta tendencia parece sostenerse en los próximos años. Abstract This research aims to analyze the competitiveness of the...

  16. Comparison and Field Validation of Binomial Sampling Plans for Oligonychus perseae (Acari: Tetranychidae) on Hass Avocado in Southern California.

    Science.gov (United States)

    Lara, Jesus R; Hoddle, Mark S

    2015-08-01

    Oligonychus perseae Tuttle, Baker, & Abatiello is a foliar pest of 'Hass' avocados [Persea americana Miller (Lauraceae)]. The recommended action threshold is 50-100 motile mites per leaf, but this count range and other ecological factors associated with O. perseae infestations limit the application of enumerative sampling plans in the field. Consequently, a comprehensive modeling approach was implemented to compare the practical application of various binomial sampling models for decision-making of O. perseae in California. An initial set of sequential binomial sampling models were developed using three mean-proportion modeling techniques (i.e., Taylor's power law, maximum likelihood, and an empirical model) in combination with two-leaf infestation tally thresholds of either one or two mites. Model performance was evaluated using a robust mite count database consisting of >20,000 Hass avocado leaves infested with varying densities of O. perseae and collected from multiple locations. Operating characteristic and average sample number results for sequential binomial models were used as the basis to develop and validate a standardized fixed-size binomial sampling model with guidelines on sample tree and leaf selection within blocks of avocado trees. This final validated model requires a leaf sampling cost of 30 leaves and takes into account the spatial dynamics of O. perseae to make reliable mite density classifications for a 50-mite action threshold. Recommendations for implementing this fixed-size binomial sampling plan to assess densities of O. perseae in commercial California avocado orchards are discussed. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  17. El aceite de aguacate y sus propiedades nutricionales

    Directory of Open Access Journals (Sweden)

    R. Pérez Rosales

    2005-01-01

    Full Text Available El aceite de aguacate, por su composición de ácidos grasos, cumple con las recomendaciones nutricionales que se enfocan a reducir la cantidad de grasa saturada en la dieta. Se caracteriza por contener una baja proporción de ácidos grasos saturados (entre un 10 y 19 por ciento, depende de la variedad y el estado de madurez, una elevada cantidad de ácido oleico (puede llegar hasta un 80 por ciento, un nivel aceptable de ácidos grasos poliinsaturados (11-15 por ciento y nada de colesterol. Se ha demostrado que las dietas enriquecidas con aceite de aguacate son tan efectivas como aquellas que contienen aceite de maíz, aceite de soya o de girasol, para reducir colesterol total, colesterol LDL (lipoproteína de baja densidad y triglicéridos del plasma, tanto en individuos hipercolesterolémicos como con diabetes mellitus tipo 2, lo cual se refleja en una disminución de los riesgos cardiovasculares. El consumo de aceite de aguacate también eleva el porcentaje de colesterol HDL (lipoproteína de alta densidad del plasma, en modelos animales. Además, el aceite de aguacate posee un grado de aterogenicidad comparable al aceite de maíz o de oliva. La efectividad del aceite de aguacate en reducir colesterol total y colesterol LDL del plasma y su bajo nivel de aterogenicidad, indican que éste puede tener un papel benéfico dentro de una dieta nutritiva

  18. Hydrodynamic Analysis of the Jaruco-Aguacate Aquifer; Analisis hidrodinamico del acuifero Jaurco-Aguacate

    Energy Technology Data Exchange (ETDEWEB)

    Dilla Salvador, Felix [Centro de Investigaciones Hidraulicas (Cuba)

    2002-03-01

    The paper shows the hydrodynamic analysis of the Jaruco-Aguacate aquifer using an unsteady groundwater flow mathematical model. The simulation includes conditions before and after pumping from a well filed, called El Gato, which is considered, as the most important water source for Havana city. The system is closed to the sea and conformed by an unconfined karstic aquifer with a well defined storage zone; its natural discharge flows through two spring groups. Results obtained with the model for the main spring discharge are near to the measured values, i. e. around 5 m{sup 3}/dec as the mean value for the period without main pumping station. Flow is reduced to 2.5-1.5 m{sup 3}/sec during pumping period but there are no data to confirm this result. Besides, a detailed study of the open boundary of the system is done when piezometric head variation in time is known. The mathematical modelling has been able to simulate the aquifer in its most general conception, in its regional as well as local representation, with a mean deviation of simulated head from observed values less than one meter in both analysed simulated periods. [Spanish] El objetivo del trabajo fue realizar un analisis hidrodinamico del acuifero Jaruco-Aguacate antes y despues de la puesta en marcha del acueducto el Gato, una de las fuentes de abasto mas importantes de la ciudad de La Habana, Cuba, basado en un modelo matematico de simulacion de flujo subterraneo transitorio bidimensional. El acuifero es una llanura carstica cerrada al mar y libre; hacia su centro, la zona de almacenamiento esta bien definida; el sistema de descarga natural realiza por medio de dos grupos de manantiales. El resultado obtenido para el gasto del manantial coincide con los valores aforados para un valor medio de 5 m{sup 3}/seg en el periodo analizado antes del funcionamiento del acueducto, pero su valor se reduce, variando desde 2.5 a 1.5 m{sup 3}/seg, en el periodo posterior, sin embargo, no existe informacion para

  19. Isotopic hydrodynamic of the aquifer systems Jaruco and Aguacate, Cuba

    International Nuclear Information System (INIS)

    Moleiro Leon, L.F.; Guerra Oliva, M.G.; Maloszewski, P.; Arellano Acosta, D.M.

    2002-01-01

    An isotopic dispersive model is applied to the Jaruco and Aguacate karstic groundwater basins of Western Cuba. The best fit of the model was validated with stable ( 18 O and 2 H ) and radioactive ( 3 H) isotopes. Modeling showed an aquifer stratification in two basic levels with turnover times of three month to upper level and close to 100 years to the lower level. The last one mainly supplies the base flow of the Ojo de agua and Bello springs and therefore, controls the dry season exploitation yields of the aqueducts of El Gato and Bello. Model results introduces an important constraint in the exploitation of groundwater resource of both aquifer systems

  20. Identificación y fluctuación poblacional de especies de trips (Thysanoptera en aguacate 'hass' en Nayarit, México

    Directory of Open Access Journals (Sweden)

    M. A. Urías-López

    2007-01-01

    Full Text Available Este estudio se realizó del año 2004 al 2006 en La Yerba (Tepic y Emiliano Zapata (Xalisco Nayarit, México. Los objetivos fueron conocer las especies de trips de inflorescencias y hojas, la dinámica poblacional en follaje y la incidencia en el follaje de árboles tratados con diferentes niveles de fertilización. Los trips fueron de las familias: Aeolthripidae (tres especies, Phlaeothripidae (dos especies y Thripidae (una especie. Se registran por primera vez en aguacate para Nayarit los trips depredadores; Erythrothrips durango, Franklinothrips vespiformis y Leptothrips mcconelli, junto con Pseudophilothrips perseae (fitófago. En La Yerba, la población más alta del complejo de trips (2.09 trips·hoja-1 ocurrió en junio; en E. Zapata, ésta se registró en julio (4.10 trips·hoja-1. Las poblaciones más altas de trips ocurrieron durante periodos de floración y crecimiento vegetativo, y más bajas durante los meses de lluvia de verano. Las poblaciones de trips fueron 60 % más altas en E. Zapata que en La Yerba. No se detectó mayor población de trips en árboles con fertilización control, respecto a los de fertilización balanceada.

  1. México en el mercado internacional de aguacate

    Directory of Open Access Journals (Sweden)

    Alejandro Macías Macías

    2011-01-01

    Full Text Available El mercado internacional de aguacate ha sufrido importantes modificaciones durante las dos últimas décadas. Por un lado, aunque se trata de una fruta exótica, cada vez es más consumida en el mundo Por otro, surgen nuevas potencias exportadoras que desplazan a anteriores naciones líderes: México, principal productor y consumidor de la fruta, es, a partir de la década de 1990, en el máximo exportador, como resultado de la apertura del mercado de los Estados Unidos. Al mismo tiempo, países sudamericanos como Chile y Perú, cada vez tienen mayor presencia en el mercado europeo, desplazando relativamente a Israel, Sudáfrica, España, Kenia o Ecuador. Finalmente, se emergen nuevos vendedores como Australia o se reafirman otros como República Dominicana.

  2. Aprovechamiento de la semilla de aguacate variedad Lorena como un colorante natural y del aceite de mesocarpios residuales de la variedad Hass como componentes funcionales en un jabón líquido

    OpenAIRE

    Hennessey Ramos, Licelander

    2017-01-01

    Tesis (Maestría en Desarrollo Sostenible y Medio Ambiente). Universidad de Manizales. Facultad de Ciencias Contables, Económicas y Administrativas. CIMAD, 2017 El presente trabajó exploró dos alternativas para el aprovechamiento de residuos generados por semilla de Persea Americana Mill Variedad Lorena, la primera, mediante la obtención de extractos acuosos tipo colorante de Agua, Solución acuosa de NaOH al 0.5% y mezcla de 50% Agua y 50% Alcohol etílico manteniendo constante la relació...

  3. Isotopic hydrodynamic of the aquifer systems Jaruco and Aguacate, Cuba; Hidrodinamica isotopica de los sistemas acuiferos Jaruco y Aguacate, Cuba

    Energy Technology Data Exchange (ETDEWEB)

    Moleiro Leon, L F; Guerra Oliva, M G [Grupo de Aguas Terrestres, Instituto de Geofisica y Astronomia, La Habana (Cuba); Maloszewski, P [GSF, Institut fue Hydrologie, Munich (Germany); Arellano Acosta, D M [Agencia de Medio Ambiente, CITMA, La Habana (Cuba)

    2002-07-01

    An isotopic dispersive model is applied to the Jaruco and Aguacate karstic groundwater basins of Western Cuba. The best fit of the model was validated with stable ({sup 18}O and 2{sup H}) and radioactive ({sup 3}H) isotopes. Modeling showed an aquifer stratification in two basic levels with turnover times of three month to upper level and close to 100 years to the lower level. The last one mainly supplies the base flow of the Ojo de agua and Bello springs and therefore, controls the dry season exploitation yields of the aqueducts of El Gato and Bello. Model results introduces an important constraint in the exploitation of groundwater resource of both aquifer systems.

  4. Agave Americana Leaf Fibers

    Directory of Open Access Journals (Sweden)

    Ashish Hulle

    2015-02-01

    Full Text Available The growing environmental problems, the problem of waste disposal and the depletion of non-renewable resources have stimulated the use of green materials compatible with the environment to reduce environmental impacts. Therefore, there is a need to design products by using natural resources. Natural fibers seem to be a good alternative since they are abundantly available and there are a number of possibilities to use all the components of a fiber-yielding crop; one such fiber-yielding plant is Agave Americana. The leaves of this plant yield fibers and all the parts of this plant can be utilized in many applications. The “zero-waste” utilization of the plant would enable its production and processing to be translated into a viable and sustainable industry. Agave Americana fibers are characterized by low density, high tenacity and high moisture absorbency in comparison with other leaf fibers. These fibers are long and biodegradable. Therefore, we can look this fiber as a sustainable resource for manufacturing and technical applications. Detailed discussion is carried out on extraction, characterization and applications of Agave Americana fiber in this paper.

  5. Insect antifeedant ryanodane diterpenes from Persea indica

    OpenAIRE

    González-Coloma, Azucena; Terrero, David; Perales, Áurea; Escoubas, Pierre; Fraga, Braulio M.

    1996-01-01

    The new ryanodane diterpenes cinnzeylanone, ryanodol 14-monoacetate, and epi-cinnzeylanol have been isolated from Persea indica(Lauraceae). The structure of cinnzeylanone has been determined by X-ray analysis. These compounds proveds to be antifeedants against Spodoptera litura. Cinnzeylanol and ryanodol were the most active antifeedants of this plant, with a range of activity close to that of ryanodine. THis is the first report on the antifeedant effects of this class of diterpenes. Their st...

  6. Tolerância a baixas temperaturas de cultivares de abacate (Persea americana Mill.

    Directory of Open Access Journals (Sweden)

    Soares Nilberto Bernardo

    2002-01-01

    Full Text Available Em experimento realizado no Núcleo de Agronomia do Sudoeste/IAC, em Capão Bonito (SP, observou-se o comportamento de 13 cultivares de abacate com seis anos de idade: Geada, Pollock e Simmonds (raça antilhana; Antoniolli, Breda, Campinas, Ermor, Jumbo, Margarida, Ouro verde, Reis e Solano (híbridos de antilhana x guatemalense e Fuerte (híbrido de mexicana x guatemalense em relação às baixas temperaturas. As temperaturas do ar registradas no posto meteorológico do local foram: -2,8degreesC; -0,4degreesC; -0,2degreesC e -1,8degreesC, respectivamente, nos dias 17; 18; 20 e 21 de julho de 2000. A cultivar Fuerte mostrou-se mais tolerante às baixas temperaturas, não apresentando injúrias nas plantas nem queda de frutos; as cultivares híbridas de antilhana x guatemalense apresentaram diferenças de tolerância ao frio; enquanto as antilhanas foram severamente injuriadas.

  7. PENGARUH PANJANG ENTRES TERHADAP KEBERHASILAN PENYAMBUNGAN TANAMAN ALPUKAT (PERSEA AMERICANA MILL.

    Directory of Open Access Journals (Sweden)

    Dirgahani Putri

    2016-06-01

    Full Text Available Beragamnya hasil produksi dan kualitas buah alpukat dapat diperbaiki dengan metode penyambungan. Penyambungan merupakan kegiatan untuk mengga-bungkan dua atau lebih sifat unggul dalam satu tanaman. Penelitian ini bertujuan mengetahui pengaruh berbagai panjang entres terhadap keberhasilan penyam-bungan tanaman alpukat. Rancangan percobaan yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok dengan lima perlakuan panjang entres, yaitu 3 cm, 6 cm, 9 cm, 12 cm, dan 15 cm.Setiap perlakuan diulang sebanyak lima kali dan setiap perlakuan terdiri atas lima tanaman;sehingga jumlah totalnya sebanyak 125 tanaman. Pengamatan dilakukan pada peubah persentase sambung hidup, jumlah tunas, panjang tunas, jumlah daun, dan diameter batang atas. Berbagai panjang entres tidak memberikan pengaruh yang nyata pada peubah persentase sambung hidup, panjang tunas terpanjang, jumlah daun, dan diameter batang atas tetapi berpengaruh nyata pada peubah jumlah tunas. Perlakuan panjang entres 15 cm memberikan hasil tertinggi untuk jumlah tunas, panjang tunas, jumlah daun dan diameter batang atas, sedangkan untuk persentase hidup perlakuan 6 cm, 12 cm memberikan hasil tertinggi yaitu 100%. Perlakuan panjang entres 3 cm adalah perlakuan yang memberikan pengaruh yang rendah pada persentase sambung hidup (yaitu 92%, peubah jumlah tunas, panjang tunas, serta diameter batang atas pada berbagai umur tanaman alpukat.

  8. Combining zygotic embryo culture and mutation induction to improve salinity tolerance in avocado (Persea americana Mill)

    Energy Technology Data Exchange (ETDEWEB)

    Fuentes, J. L.; Santiago, L.; Alvarez, A.; Valdés, Y.; Vernhe, M.; Guerra, M.; Altanez, S.; Prieto, E. F. [Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Miramar, Playa, C. Habana (Cuba); Rodríguez, N. N.; Arbelo, O. Coto; Velázquez, B.; Rodríguez, J. A.; Sourd, D. G.; Fuentes, V. R. [Instituto de Investigaciones de Fruticultura Tropical (IICF), Miramar, Playa, C. Habana (Cuba); Leal, M. R. [Departamento de Microbiología, Facultad de Biología, Universidad de la Habana, Vedado, C. Habana (Cuba)

    2009-05-15

    Mutation induction and biotechnological techniques are some of the current approaches used in plant breeding. In the present work radiation-induced mutation followed by in vitro culture of zygotic embryos and high osmotic pressure selection methods to improve salt tolerance in avocado are investigated. The in vitro germination, rooting, bud multiplication and plantlet acclimatization of Cuban avocado varieties were recorded. The germination rates of whole embryos in vitro were found to be higher when using mature rather than immature embryos. Almost 80% of the whole embryos derived plantlets produced were successfully acclimatized under greenhouse conditions. An in vitro propagation method for avocado breeding purposes was optimized and documented. However, in vitro multiplication results indicated the need to improve bud multiplication methods in avocado. The survival rates of gamma rays irradiated and salt pressured avocado embryos were also investigated. Both mutagenic (LD{sub 50} = 27-28 Gy) and selective (LD{sub 20} = 157 mM of NaCl) doses were established. A procedure combining zygotic embryo culture and mutation induction was used to obtain. Putative mutant lines derived from salt tolerant rootstocks were developed. Putative M{sub 1}V{sub 3} progenies were planted in the field for segregation analysis. An avocado gene bank was established under the same study. Therefore this methodology appears as an alternative to traditional breeding methods, particularly for improving agronomic characteristics such as salt tolerance in avocado. (author)

  9. The potential of avocado paste (Persea americana) as fat substitute in non-dairy ice cream

    Science.gov (United States)

    Ervina; Surjawan, I.; Abdillah, E.

    2018-01-01

    Consumer preferences towards plant-based food have shifted significantly due to sustainable and healthy reasons. Dairy products consist of high Saturated Fatty Acid (SFA) and overconsumption of SFA could lead to cardiovascular diseases. Avocado contains high levels of fat dominated by Monounsaturated Fatty Acid (MUFA) and phytosterol that have the potential as a plant-based fat source to substitute dairy-fat in ice cream. The objective of this study was to analyze the physicochemical, rheological and sensorial properties of ice cream substituted with different concentrations of avocado paste ranging from 0%, 25%, 50%, 75% and 100% respectively against dairy fat to produce non-dairy fat ice cream. The psychochemical properties and total fat were determined. Sensorial quality and hedonic attributes of ice cream were investigated using 60 semi-trained panelists. There were significant differences (p0.05). The addition of 50% avocado paste was the most preferred among the panelists. Avocado could provide a potential substitution for dairy-fat in ice cream.

  10. Effects of Avocado (Persea americana) on Metabolic Syndrome: A Comprehensive Systematic Review.

    Science.gov (United States)

    Tabeshpour, Jamshid; Razavi, Bibi Marjan; Hosseinzadeh, Hossein

    2017-06-01

    Metabolic syndrome (MetS) is a clustering of risk factors including high blood glucose, dyslipidemia, hypertension, and obesity that lead to the increased risk of type 2 diabetes mellitus and cardiovascular diseases (CVDs), which are among leading causes of death in the world. Metabolic syndrome increases the risk of type 2 diabetes mellitus and CVDs by approximately five and three folds, respectively. Therefore, it is of vital importance to manage such conditions with herbal options which have less undesirable adverse effects and may be more efficacious in comparison with synthetic options. Avocado is a well-known source of carotenoids, minerals, phenolics, vitamins, and fatty acids. The lipid-lowering, antihypertensive, antidiabetic, anti-obesity, antithrombotic, antiatherosclerotic, and cardioprotective effects of avocado have been demonstrated in several studies. In this review, we aimed to find out avocado's pharmacological effects on different components of MetS. Moreover, this review report is performed on the MetS effects of peel, seed, flesh, and leaves of avocado. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  11. Inter-firm coordination in the Mexican avocado (Persea americana) industry: the packer-buyer relationship

    NARCIS (Netherlands)

    Arana Coronado, J.J.; Bijman, J.; Omta, S.W.F.; Oude Lansink, A.G.J.M.

    2012-01-01

    Vertical coordination is an effective response to uncertainties in marketing environments. This article presents evidence relating the influence of transaction characteristics on interfirm coordination to respond what conditions determine the level of inter-firm coordination between packers and

  12. The Spot Problem in Avocado (Persea americana Mill. Plantations of Northern Tolima, Colombia

    Directory of Open Access Journals (Sweden)

    Julián Reina Noreña

    2015-07-01

    Full Text Available The spot (common name is currently the main phytosanitary problem in avocado plantations of northern Tolima. In Colombia, information about its causal agent and the external factors that foster its development is scarse, which lead us to carry on a preliminary research. In three avocado farms of Fresno municipality, the severity of the spot in Lorena cultivar fruits was evaluated by using five types of barriers and a control, from the inflorescence phase to the developed fruit. The symptoms of the “avocado spot” in Lorena’ cultivar were characterized. Samples from different plant organs were sent to four diagnostic laboratories. Besides, in ten farms of Fresno municipality, the severity of the spot was measured on Lorena, Hass and Choquette cultivars by carrying out bi-weekly samplings. Simultaneously, in eleven farms in Fresno, the culture labors, pesticides, production costs and incomes were recorded. The treatment that was significantly different from the control was the fungicide application. The phytopathological diagnosis identified two pathogens, Glomerella sp. (Anamorphic: Colletotrichum sp. and Cercospora sp. related to the spot symptoms. The Lorena cultivar was the most affected by “the spot”, it caused an increase of 3.65 % of the production costs to growers and the crop incomes decreased 21.65 % due to fruits with “spot” symptoms. An environmental impact was perceived as a result of the grower lack of awareness about the problem.

  13. Effect of semisolid formulation of persea americana mill (avocado) oil on wound healing in rats.

    Science.gov (United States)

    de Oliveira, Ana Paula; Franco, Eryvelton de Souza; Rodrigues Barreto, Rafaella; Cordeiro, Daniele Pires; de Melo, Rebeca Gonçalves; de Aquino, Camila Maria Ferreira; E Silva, Antonio Alfredo Rodrigues; de Medeiros, Paloma Lys; da Silva, Teresinha Gonçalves; Góes, Alexandre José da Silva; Maia, Maria Bernadete de Sousa

    2013-01-01

    The aim of this study was to evaluate the wound-healing activity of a semisolid formulation of avocado oil, SSFAO 50%, or avocado oil in natura, on incisional and excisional cutaneous wound models in Wistar rats. An additional objective was to quantify the fatty acids present in avocado oil. On the 14th day, a significant increase was observed in percentage wound contraction and reepithelialization in the groups treated with 50% SSFAO or avocado oil compared to the petroleum jelly control. Anti-inflammatory activity, increase in density of collagen, and tensile strength were observed inSSFAO 50% or avocado oil groups, when compared to control groups. The analysis of the components of avocado oil by gas chromatography detected the majority presence of oleic fatty acid (47.20%), followed by palmitic (23.66%), linoleic (13.46%) docosadienoic (8.88%), palmitoleic (3.58%), linolenic (1.60%), eicosenoic (1.29%), and myristic acids (0.33%). Our results show that avocado oil is a rich source of oleic acid and contains essential fatty acids. When used in natura or in pharmaceutical formulations for topical use, avocado oil can promote increased collagen synthesis and decreased numbers of inflammatory cells during the wound-healing process and may thus be considered a new option for treating skin wounds.

  14. Inventarisasi Alpukat (Persea americana Mill) pada Berbagai Ketinggian di Kabupaten Bener Meriah Provinsi Nanggroe Aceh Darussalam

    OpenAIRE

    Putra, Nopriza

    2011-01-01

    There are several benefits of avocados in the Bener Meriah Regency that are not owned avocados in other regions. Therefore, a research was conducted the distribution avocados at different elevations in the area in July-August 2010 by using descriptive method. Parameters measured were the large number of plant avocado, avocado species, pests and diseases, and productivity of avocado plants found on altitude. The results showed that the large number of avocado is not based on altitude above sea...

  15. Activity-guided identification of acetogenins as novel lipophilic antioxidants present in avocado pulp (Persea americana).

    Science.gov (United States)

    Rodríguez-Sánchez, Dariana; Silva-Platas, Christian; Rojo, Rocío P; García, Noemí; Cisneros-Zevallos, Luis; García-Rivas, Gerardo; Hernández-Brenes, Carmen

    2013-12-30

    Avocado fruit is a rich source of health-related lipophilic phytochemicals such as monounsaturated fatty acids, tocopherols, carotenes, acetogenins and sterols. However, limited information is available on the contribution of specific phytochemicals to the overall antioxidant capacity (AOC) of the fruit. Centrifugal partition chromatography was used as fractionation tool, guided by an in vitro chemical assay of oxygen radical absorbance capacity (ORAC). Subsequent experiments focused on isolation and characterization of the chemical nature of the main contributors to lipophilic AOC of avocado pulp. ORAC values obtained for acetogenins were contrasted with results from an isolated kidney mitochondria membrane lipid peroxidation bioassay. The present study established that lipophilic AOC of the pulp was significantly higher than its hydrophilic AOC. Our results confirmed the presence of acetogenins in the fractions with highest lipophilic AOC, and for the first time linked them as contributors to lipophilic-ORAC values. Further HPLC-PDA/MS-TOF analysis led to structural elucidation of two novel acetogenins, not previously reported as present in avocado pulp, along with five already known related-compounds. Antioxidant properties observed for avocado pulp acetogenins by the ORAC assay suggested that, in the presence of an emulsifying agent, acetogenins could serve as novel lipophilic antioxidants in a food matrix. Results from isolated mitochondria lipid peroxidation bioassay, indicated that L-ORAC values which may have relevance for food matrix applications, should not be interpreted to have a direct relevance in health-related claims, compounds need to be evaluated considering the complexity of biological systems. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Growth and abscission of avocado fruits (Persea americana Mill. cv. Fuerte

    Directory of Open Access Journals (Sweden)

    Rodolfo B.Muñoz Perez

    2013-12-01

    Full Text Available In a commerical orchard of 18-year-old trees, 1250 fruits were chosen for observation. The majority of them abscised during the first weeks after anthesis. Afterwards, the abscission gradually descreased, however, in the meantime three additional waves of more intensive shedding occurred which were best visible when the Relative Abscission Rate (RAR was calculated. The peaks in the RAR curve were preceded by a decrease in the Relative Growth Rate (RGR of persistent fruits, which suggests that nutritional or other stresses occurred in the whole branch. In the period from May 15th to July 15th, predominantly the smaller fruits were abscised, later on, however, fruits of the same size as the persistent ones were shed. The formula for calculating RAR and RAR is shortly discussed.

  17. Pemanfaatan Tepung Buah Alpukat (Persea americana Mill.) dalam Pembuatan Bolu Terhadap Daya Terima dan Kandungan Gizinya

    OpenAIRE

    Regar, Nurhalis Br

    2015-01-01

    Food diversification efforts can be done by using local food. Avocado is one of the local food that have many nutrients and its availability is quite promising because the production of avocado tend to increase every year. Avocado processed into flour as substitute the wheat flour in the manufacture of cake. Avocado flour cake is one of the food product that modified by addingflouravocado and contain nutrient that needed the body. The purpose of this researchis to know the a...

  18. Analyses of avocado (Persea americana) nectar properties and their perception by honey bees (Apis mellifera).

    Science.gov (United States)

    Afik, O; Dag, A; Kerem, Z; Shafir, S

    2006-09-01

    Honey bees are important avocado pollinators. However, due to the low attractiveness of flowers, pollination is often inadequate. Previous work has revealed that avocado honey is relatively unattractive to honey bees when compared with honey from competing flowers. We characterized avocado honey and nectar with respect to their odor, color, and composition of sugars, phenolic compounds, and minerals. Furthermore, we tested how honey bees perceive these parameters, using the proboscis extension response bioassay and preference experiments with free-flying bees. Naïve bees were indifferent to odors of avocado and citrus flowers and honey. Experienced bees, which were collected in the field during the blooming season, responded preferentially to odor of citrus flowers. The unique sugar composition of avocado nectar, which contains almost exclusively sucrose and a low concentration of the rare carbohydrate perseitol, and the dark brown color of avocado honey, had no negative effects on its attractiveness to the bees. Phenolic compounds extracted from avocado honey were attractive to bees and adding them to a solution of sucrose increased its attractiveness. Compared with citrus nectar and nonavocado honey, avocado nectar and honey were rich in a wide range of minerals, including potassium, phosphorus, magnesium, sulfur, iron, and copper. Potassium and phosphorus, the two major minerals, both had a repellent effect on the bees. Possible explanations for the presence of repellent components in avocado nectar are discussed.

  19. Gamma rays induced variability in mature embryos of avocado (Persea americana Mill)

    International Nuclear Information System (INIS)

    Fuentes, J.L.; Ramirez, I. M; Santiago, L.; Valdes, Y.; Guerra, M; Prieto, E.; Rodriguez, N.; Velazquez, B.

    2001-01-01

    Induced mutation and biotechnology techniques are current approaches used in plant breeding. At present work, the induced mutation and embryo zygotic culture techniques were used in order to characterize the radiosensitivity of avocado commercial varieties, Hass and California. The induced diversity in plant material was also evaluated in morphological seedling descriptors as: height seedling, diameter seedling neck, leaves number, length of principal root and secondary root number. The obtained results showed high susceptibility of both varieties to gamma rays. California was the higher sensitivity variety. Percentage of entire shoot induction showed clear dependence of radiation dose in both varieties. Thus dose range for mutagenesis was determined. In general, variation of morphological seedling descriptors not was clearly agreed to increase of radiation dose. In addition, the results suggested that variation in morphological seedling descriptors also could be depending of genotypes. The useful of mature embryo culture of avocado for improvement of breeding approaches in this crop, was discussed

  20. Whitefly (Paraleyrodes sp. preference for avocado cultivars (Persea americana Mill. in Fresno, Tolima

    Directory of Open Access Journals (Sweden)

    Paola Vanessa Sierra

    2014-07-01

    Full Text Available The whitefly is a major pest that affects avocado and there is not enough information about the biolog y and management of this insect in Colombia. In this paper the incidence of the whitefly Paraleyrodes sp. pos. bondarion four avocado cultivars was studied (Choquette, Hass, Lorena, Santana. The crop management carried out by farmers was characterized and its effect on the white fly presence on three cultivars was evaluated (Choquette, Hass, Lorena. Presence of secondary metabolites on leaves from Hass and Lorena was measured and the effect of their leaf extracts on the mortality and repellency index of whitefly adults was established. There were interactions between crop management and cultivar for the presence of the insect; moderately chemical management had the lowest infestation in all cultivars. The increase in soil fertilization and chemical insecticides increased insect population, and removal of the hemiparasite decreased it. Lorena cultivar had the highest fly preference, insect population increase when there was interaction between cultivar and mature leaves, neutral repellency index, and high percentage of total and non-reducing carbohydrates. The Hass cultivar showed higher amount of coumarins, terpenes and steroids, and high rate of repellency. Lorena attracting the insect is due to the presence of carbohydrates; and Hass repellency is due to the terpene and / or steroids. Research works should be done on whitefly in avocado crops toward cultivars Lorena

  1. Chemical composition, toxicity and larvicidal and antifungal activities of Persea americana (avocado) seed extracts.

    Science.gov (United States)

    Leite, João Jaime Giffoni; Brito, Erika Helena Salles; Cordeiro, Rossana Aguiar; Brilhante, Raimunda Sâmia Nogueira; Sidrim, José Júlio Costa; Bertini, Luciana Medeiros; Morais, Selene Maia de; Rocha, Marcos Fábio Gadelha

    2009-01-01

    The present study had the aim of testing the hexane and methanol extracts of avocado seeds, in order to determine their toxicity towards Artemia salina, evaluate their larvicidal activity towards Aedes aegypti and investigate their in vitro antifungal potential against strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis through the microdilution technique. In toxicity tests on Artemia salina, the hexane and methanol extracts from avocado seeds showed LC50 values of 2.37 and 24.13 mg mL-1 respectively. Against Aedes aegypti larvae, the LC50 results obtained were 16.7 mg mL-1 for hexane extract and 8.87 mg mL-1 for methanol extract from avocado seeds. The extracts tested were also active against all the yeast strains tested in vitro, with differing results such that the minimum inhibitory concentration of the hexane extract ranged from 0.625 to 1.25mg L-(1), from 0.312 to 0.625 mg mL-1 and from 0.031 to 0.625 mg mL-1, for the strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis, respectively. The minimal inhibitory concentration for the methanol extract ranged from 0.125 to 0.625 mg mL-1, from 0.08 to 0.156 mg mL-1 and from 0.312 to 0.625 mg mL-1, for the strains of Candida spp., Cryptococcus neoformans and Malassezia pachydermatis, respectively.

  2. Enzymatic browning in avocado (Persea americana) revisited: History, advances, and future perspectives.

    Science.gov (United States)

    Toledo, Lea; Aguirre, Carolina

    2017-12-12

    Considering nearly 80 years of research regarding one of the enzymes responsible for catalyzing the formation of pigments in higher animals, plants, fungi and bacteria, this review will focus on collecting and categorizing the existing information about polyphenol oxidase (PPO) in fruits, with particular emphasis on the information in relation to avocado, which is one of the hardiest species in terms of inactivation, has documented dual activity (EC 1.14.18.1/EC 1.10.3.1), and represents one of the oldest challenges for food science research and fruit processors. It is expected that this review will contribute to the further development of the field by highlighting the questions that have arisen during the characterization of PPO, the progress that has been made and the questions that remain today, in addition to new methodologies that are being applied to study this system. Holistic methodologies offer unexplored potential for advancing our understanding of the complex phenomena that govern PPO activity in fruits, because these methodologies will enable the characterization of this family of enzymes in all of its complexity. Subsequently, it will be possible to develop better techniques for controlling enzymatic browning in this valuable fruit.

  3. Hypolipidemic effect of avocado (Persea americana Mill) seed in a hypercholesterolemic mouse model.

    Science.gov (United States)

    Pahua-Ramos, María Elena; Ortiz-Moreno, Alicia; Chamorro-Cevallos, Germán; Hernández-Navarro, María Dolores; Garduño-Siciliano, Leticia; Necoechea-Mondragón, Hugo; Hernández-Ortega, Marcela

    2012-03-01

    Avocado seed contains elevated levels of phenolic compounds and exhibits antioxidant properties. We investigated the effect of Avocado Seed Flour (ASF) on the lipid levels in mice on a hyperlipidemic diet. The concentration of phenols was determined by high-performance liquid chromatography, antioxidant activity was evaluated using the Trolox equivalent antioxidant capacity method, and dietary fiber was measured using the Association of Official Analytical Chemists (AOAC) method. The LD50 of ASF was determined using Lorke's method and hypolipidemic activity was evaluated in a hypercholesterolemic model in mice. Protocatechuic acid was the main phenolic compound found in ASF, followed by kaempferide and vanillic acid. The total phenolic content in the methanolic extract of ASF was 292.00 ± 9.81 mg gallic acid equivalents/g seed dry weight and the antioxidant activity resulted in 173.3 μmol Trolox equivalents/g DW. In addition, a high content of dietary fiber was found (34.8%). The oral LD50 for ASF was 1767 mg/kg body weight, and treatment with ASF significantly reduced the levels of total cholesterol, LDL-C, and prediction of the atherogenic index. Therefore, the antioxidant activity of phenolic compounds and dietary fiber in ASF may be responsible for the hypocholesterolemic activity of ASF in a hyperlipidemic model of mice.

  4. NUTRITIONAL AND HORMONAL FACTORS AFFECTING FRUIT SET IN AVOCADO (Persea americana Mill.)

    OpenAIRE

    D'ASARO, ANTONIO

    2017-01-01

    Under favourable conditions, the avocado sets more fruits than the tree is able to bring to maturity, so that the plant adjusts, during the early stages of development, its ability to nourish them by modifying their number, that is, causing the fruit drop of those who can not maintain their growth rate. Accordingly, carbohydrate availability could be a key factor in the physiological abscission of these fruits. Since this species presents dichogamy, the abscission of fruits has also been attr...

  5. The contribution of honey bees, flies and wasps to avocado (Persea americana pollination in southern Mexico

    Directory of Open Access Journals (Sweden)

    Jesica Perez-Balam

    2012-06-01

    Full Text Available Although avocado is native to Mexico, there are no comparative measures in this country on the performance of its flower visitors as pollinators. The contribution of honey bees, flies and wasps to the pollination of avocado from tropical Mexico was assessed by comparing abundance, speed of flower visitation, quantity of pollen carried per individual and pollen deposited on virgin flowers after single visits. The values of abundance and frequency of flower visitation with pollen deposition were combined to obtain a measure of pollinator performance (PP. The most abundant insects on avocado were flies (mean ± SE: 15. 2 ± 6.2, followed by honey bees (9.4 ± 6.3 and wasps (4.2 ± 3.1 (ANOVA F = 91.71, d.f. = 2,78; P P P = 0.001, the number of pollen grains deposited on a stigma after a single visit was similar for the three taxa (2-5. There was evidence for a significant and similarly positive PP of both honey bees and flies as avocado pollinators over wasps, given their abundance, potential for pollen transport and deposition of pollen on stigmas.

  6. Physicochemical parameters of avocado Persea americana Mill. cv. Hass (Lauraceae grown in Antioquia (Colombia for export

    Directory of Open Access Journals (Sweden)

    Pablo Rodriguez

    2018-05-01

    Full Text Available Hass avocado is transported maintaining an unbroken cold chain and in the destination market, it is ripened under controlled conditions. Fruit quality depends on preharvest, factors, harvest time and postharvest treatments. Colombia has poor information on quality parameters despite the current growing and fruit export boom. Therefore, the aim of this study was to determine some important fruit quality parameters. For that, Hass avocado fruits were harvested considering three maturity indexes form exporting orchards in Antioquia, where fruits were stored and ripened. Temperature, relative humidity and shipment times to the European market were simulated. Dry matter content was used as harvest index. Soluble solids, pH, and titratable acidity were analyzed as quality parameters. Fruit origin, harvest index and storage time had a significant effect on quality parameters of avocado (p≤0.05. There was an increase in Brix degrees (5.07 to 7.26 and pH (6.58 to 7.14 throughout the storage time until the fourth week, where these decreased. In contrast, acidity dropped (19.47 to 9.24% with storage time. These quality parameters can be considered quality indicators; however, more studies are required.

  7. Physicochemical parameters of avocado Persea americana Mill. cv. Hass (Lauraceae) grown in Antioquia (Colombia) for export

    OpenAIRE

    Pablo Rodriguez; Camilo Astudillo

    2018-01-01

    Hass avocado is transported maintaining an unbroken cold chain and in the destination market, it is ripened under controlled conditions. Fruit quality depends on preharvest, factors, harvest time and postharvest treatments. Colombia has poor information on quality parameters despite the current growing and fruit export boom. Therefore, the aim of this study was to determine some important fruit quality parameters. For that, Hass avocado fruits were harvested considering three maturity indexes...

  8. Análisis nutricional y estudio de la actividad antioxidante de algunas frutas tropicales cultivadas en Colombia

    OpenAIRE

    Moreno Gómez, Elizabeth

    2014-01-01

    El objetivo principal de este trabajo consistió en realizar el análisis nutricional que incluyo: porcentaje de humedad, cenizas, proteína, grasa, fibra dietaria total, minerales y carbohidratos, además se evaluó la actividad antioxidante de extractos de pulpa de gulupa (Passiflora edulis Sims.), curuba (Passiflora tripartida var. Mollissima), aguacate (Persea americana Mill.), uchuva (Physalis peruviana L.), tomate de árbol (Cyphomandra betacea Sendt.) y lulo (Solanum quitoense...

  9. Extracción de aceite de aguacate: Un experimento industrial

    Directory of Open Access Journals (Sweden)

    Martínez Nieto, L.

    1992-02-01

    Full Text Available The results of an industrial experiment to obtain avocado oil by continuous process similar to that for obtaining olive oil are analyzed. Possible ways of improving the method and thus the yield are pointed out.

    Se analizan los resultados obtenidos en un experimento industrial de obtención de aceite de aguacate por un proceso continuo similar al de obtención de aceite de oliva, indicando las posibles modificaciones para mejorar el rendimiento.

  10. “INCURSIÓN EN LOS MERCADOS INTERNACIONALES DE LAS EMPRESAS EXPORTADORAS DE AGUACATE DE MICHOACÁN”

    OpenAIRE

    GIL BOTELLO, JAIME ANTONIO

    2012-01-01

    El aguacate se ha convertido en una de las frutas de mayor crecimiento y dinamismo comercial a nivel mundial. La evidencia empírica señala que las empresas exportadoras de aguacate en Michoacán participan activamente en la dinámica comercial del estado y del país, ya sea, produciendo, comercializando o exportando tienen presencia internacional en mercados como: Estados Unidos, Japón, Canadá, Francia, Holanda, Inglaterra, Alemania y Centroamérica; en los últimos ocho años el ...

  11. COMPETITIVIDAD, RECONVERSIÓN PRODUCTIVA Y MERCADO INTERNACIONAL DEL AGUACATE ORGÁNICO MICHOACANO, 2004-2006

    OpenAIRE

    VILLAFÁN VIDALES, KATIA BEATRIZ

    2012-01-01

    La agricultura orgánica ha tomado una importancia a nivel mundial los últimos dos años, debido a la demanda creciente y a los beneficios sociales, económicos y ecológicos que representa. Esta investigación surge a partir la necesidad de conocer la situación de la agricultura orgánica en el estado de Michoacán, específicamente en el aguacate. Los objetivos centrales de la investigación son: determinar los factores de competitividad de los productores de aguacate orgánico; conoce...

  12. Persea declinata (Bl. Kosterm Bark Crude Extract Induces Apoptosis in MCF-7 Cells via G0/G1 Cell Cycle Arrest, Bcl-2/Bax/Bcl-xl Signaling Pathways, and ROS Generation

    Directory of Open Access Journals (Sweden)

    Putri Narrima

    2014-01-01

    Full Text Available Persea declinata (Bl. Kosterm is a member of the Lauraceae family, widely distributed in Southeast Asia. It is from the same genus with avocado (Persea americana Mill, which is widely consumed as food and for medicinal purposes. In the present study, we examined the anticancer properties of Persea declinata (Bl. Kosterm bark methanolic crude extract (PDM. PDM exhibited a potent antiproliferative effect in MCF-7 human breast cancer cells, with an IC50 value of 16.68 µg/mL after 48 h of treatment. We observed that PDM caused cell cycle arrest and subsequent apoptosis in MCF-7 cells, as exhibited by increased population at G0/G1 phase, higher lactate dehydrogenase (LDH release, and DNA fragmentation. Mechanistic studies showed that PDM caused significant elevation in ROS production, leading to perturbation of mitochondrial membrane potential, cell permeability, and activation of caspases-3/7. On the other hand, real-time PCR and Western blot analysis showed that PDM treatment increased the expression of the proapoptotic molecule, Bax, but decreased the expression of prosurvival proteins, Bcl-2 and Bcl-xL, in a dose-dependent manner. These findings imply that PDM could inhibit proliferation in MCF-7 cells via cell cycle arrest and apoptosis induction, indicating its potential as a therapeutic agent worthy of further development.

  13. amylase from cockroach, Periplaneta americana

    African Journals Online (AJOL)

    STORAGESEVER

    2008-08-04

    Aug 4, 2008 ... The enzyme was stable up to 55°C and its pH stability was in range of 5.6 - 6.6. .... acrylamide gel and by gel filtration chromatography. Gel filtration .... Activity at 55 °C. Figure 8. Thermic inactivation at 37°C and 55°C of Periplaneta americana α-amylase. Thermal stability of the enzyme was followed for 1 h.

  14. Genipa americana L.: technological prospecting

    Directory of Open Access Journals (Sweden)

    Selma Maria Santos Moura

    2016-10-01

    Full Text Available Genipap is a tall tree (10 to 15 meters, found in humid tropical regions, common in brazilian northeastern. Its scientific name is Genipa americana L., jenipapo comes from the Tupi-Guarani jandipap, which means fruit that serves to paint. It features straight stem, dark green leaves, golden-yellow flowers and fruit in the form of ovoid berry, has light brown pulp surrounding seeds in the center. In food, its fruit is edible natural and in the preparation of sweets, soft drinks and wine. It is rich in iron, vitamins B1, B2, B5 and C, calcium and carbohydrates. In popular culture have medicinal indications for the treatment of diseases. When green, the fruit provides a blue juice widely used as a colorant, transparent at first, which becomes black when oxidized, has consistency of ink and body in contact with the skin, it leaves stains that disappear after a week or more spontaneously. The objective of this research was to carry out technology foresight regarding Genipa americana L., to present an overview of the technologies developed related to the theme. For that held a patent search on the basis of the European Patent Office, World Intellectual Property Organization, United States Patent and Trademark Office and the National Institute of Industrial Property. Using the Genipa americana L. keyword were registered 88 patents, when added the word dye 5 patent were found and no request when the search was conducted using Genipa americana L. and cell. Brazil recorded a lower number of patents found in the USA and Japan, with the years 2010, 2011 and 2015, which showed a higher number. Integrated actions should be taken to encourage development institutions to research in order to enable an environment conducive to the generation of innovations, making Brazil a more competitive country in the technological point of view.

  15. Estudios etiológicos de la marchitez del aguacate en Antioquia-Colombia

    Directory of Open Access Journals (Sweden)

    Joaquín Guillermo Ramírez Gil

    2014-02-01

    Full Text Available El cultivo de aguacate en Colombia ha presentado un incremento en el área sembrada durante la última década, especialmente en clima frío moderado, entre 1.800 y 2.500 msnm. La variedad Hass se destaca por tener la mayor área sembrada en este clima, en el departamento de Antioquia-Colombia. Este cultivo presenta grandes retos tecnológicos para su expansión; entre ellos el manejo de enfermedades ocupa un lugar predominante por las implicaciones que tiene en costos, impacto en la salud, ambiente y restricciones para exportaciones. La marchitez, ocasiona la muerte de numerosos árboles en todas las etapas del cultivo y presenta la mayor incidencia y severidad de las enfermedades identificadas para este cultivo. El desconocimiento de los agentes causales de esta enfermedad, ha llevado a realizar prácticas de manejo encaminadas principalmente al control del oomycete Phytophthora cinnamomi Rands, como único agente causal. Esta investigación tuvo como objetivo identificar los distintos agentes causales de la marchitez del aguacate y la relación de su incidencia con las prácticas de manejo en lotes cultivados en el departamento de Antioquia-Colombia. Los resultados encontrados muestran que la marchitez es el principal problema del cultivo de aguacate. Los microorganismos P. cinnamomi, Phytophthora heveae Thompson, Phytophthora citrícola Sawada, Verticillium sp., y Cylindrocarpon destructans (Zinss Scholten fueron aislados y reprodujeron los síntomas asociados a marchitez. Las condiciones de bajo contenido de oxigeno el suelo también reprodujeron la sintomatología de marchitez, involucrando también un origen abiótico en la etiología.

  16. Evaluation gives productivity and quality gives fruit in Aguacate subjected has to gamma radiation; Evaluacion de productividad y calidad de fruta en aguacate hass sometido a radiacion gamma

    Energy Technology Data Exchange (ETDEWEB)

    De La Cruz Torres, Eulogio; Garcia Andrade, Juan M [Instituto Nacional de Investigaciones Nucleares. Mexico D.F. (Mexico); Ibannez Palacios, Jorge [Facultad de Ciencias Agricolas. U.A.E., Toluca (Mexico); Mijares Oviedo, Pedro [Fundacion Salvador Sanchez Col CICTAMEX, Coatepec Harina (Mexico)

    1999-07-01

    Evaluation of productivity, post harvest behavior and fruit quality was performed on four years Has avocado trees irradiated with 60 Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the La Labor Experimental Center of the Centro de Investigaciones Cientificas y Tecnologicas del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300%. In regard to post harvest performance, the respiration index (mg CO2 /kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This means that radiation has altered productivity but not the quality and post harvest behavior of fruits.

  17. Efeito do abacate (Persea americana Mill variedade hass na lipidemia de ratos hipercolesterolêmicos Effect of the hass avocado (American Persea Mill on hipercolesterolemic rats

    Directory of Open Access Journals (Sweden)

    Jocelem Mastrodi Salgado

    2008-12-01

    Full Text Available Os fitonutrientes podem ser encontrados em muitas frutas, sendo o abacate o mais rico em beta-sitosterol (fitoesterol e glutationa. O fitoesterol é uma substância de origem vegetal cuja estrutura é muito similar a do colesterol e seu mecanismo de ação envolve a inibição intestinal de absorção do colesterol e diminuição da síntese de colesterol hepático. O efeito é exercido sobre os valores de colesterol plasmático total e LDL, sem afetar os níveis de HDL e triglicérides sanguíneos. O presente trabalho teve como objetivo analisar a influência do consumo de farinha de abacate variedade Hass nos níveis séricos de colesterol total, HDL, LDL, triglicérides, colesterol hepático e excretado. Estes parâmetros foram investigados em ratos Wistar hipercolesterolêmicos durante 30 e 60 dias de experimento. Ao final de 30 dias, a dieta com 15% de abacate mostrou ser mais efetiva para reduzir os níveis de colesterol total e LDL e aumentar o HDL em comparação ao controle. Foi observado que para o teor de colesterol excretado a melhor dieta foi a de 25% de abacate, pois quanto maior a concentração de abacate, maior a excreção de colesterol. A dieta com 15% e 25% de abacate, foram as que mais influenciaram nos níveis de colesterol hepático.Phytonutrients can be found in fruits, and the avocado contains four times more beta-sitosterol (phytosterol and it is one of the best glutathione sources. The phytosterol is a vegetable substance whose structure is very similar to the cholesterol one and its action mechanism involves the intestinal inhibition of cholesterol absorption and the synthesis of hepatic cholesterol. The effect has an influence on the values of total plasmatic cholesterol and LDL without affecting the levels of HDL and triglycerides. The present work had the objective to analyze the influence of the consumption of the Hass avocado on the levels of total cholesterol, HDL, LDL, triglycerides, and hepatic and excreted cholesterol. These parameters were investigated in hipercholesterolemic Wistar rats during 30 and 60 days of experiment. At the end of 30 days, the 15% avocado diet reduced the levels of total cholesterol and LDL in comparison to the control. It was observed that for the excreted cholesterol, the best diet was the 25% of avocado since the cholesterol excretion increased with the increase of the avocado concentration. The 15% avocado diet also influenced the levels of hepatic cholesterol.

  18. FRIGOCONSERVACIÓN Y ATMÓSFERAS MODIFICADAS EN FRUTOS DE AGUACATE MÍNIMAMENTE PROCESADO

    Directory of Open Access Journals (Sweden)

    J. A. Zarazúa-Escobar

    2005-01-01

    Full Text Available Este estudio tuvo como objetivo evaluar el efecto de dos atmósferas modificadas, una generada por polietileno de baja densidad lineal y el uso de Vacum 300, a dos temperaturas de almacenamiento 5 y 20 °C, así como el uso de antioxidantes (B-tocoferol, ácido Lascórbico y butilhidroxi-tolueno en la prolongación de la vida de anaquel de aguacate mínimamente procesado. Se evaluó la pérdida fisiológica de peso, color, firmeza, daños por frío, potencial de obscurecimiento, compuestos fenólicos, actividad de la polifenoloxidasa, incidencia y severidad de enfermedades, y evaluación sensorial. El mejor tratamiento fue el de película para vacío, Vacum 300 con antioxidantes (B-tocoferol, ácido L-ascórbico y butilhidroxi-tolueno almacenado a 5 °C, el cual tuvo la capacidad de mantener un aroma y apariencia aceptables en las rebanadas de aguacate. También redujo de forma considerable la pérdida fisiológica de peso, aminoró los desórdenes causados por el frío y conservó al producto en perfectas condiciones (libre de hongos a lo largo de 13 días.

  19. Ewingella Americana: An Emerging True Pathogen

    Directory of Open Access Journals (Sweden)

    Syed Hassan

    2012-01-01

    Full Text Available Infections caused by Ewingella americana have been rarely reported in the literature. Most of the cases that have been reported were among the immunocompromised patients. We report a case of E. americana causing osteomyelitis and septic arthritis of the shoulder joint in a previous intravenous drug abuser. The causative pathogen was identified by synovial fluid analysis and culture.

  20. VARIACIÓN ESTACIONAL DE LA FOTOSÍNTESIS EN VARIOS CULTIVARES DE AGUACATE

    Directory of Open Access Journals (Sweden)

    Medina-Torres R

    2011-07-01

    Full Text Available Se estudió la variación estacional de la fotosíntesis y parámetros fisiológicos relacionados con ésta en los cultivares de aguacate Colín V-101 y Rincón (ambos de bajo porte y Hass en el clima Semicálido subhúmedo del Valle de Matatipac, Nayarit. Se midió la fotosíntesis en seis fechas de muestreo y en cada una de ellas fueron evaluadas cinco hojas maduras en cada uno de cinco árboles por cultivar. La primera medición se hizo al fin de verano (21 Sep. 2002, dos lecturas en otoño (20 Oct. y 20 Nov. 2002, dos en invierno (22 Ene. y 23 Feb. 2003 y una al inicio de la primavera (30 Mar. 2003. La dispersión estacional de la tasa de fotosíntesis neta (A de los tres cultivares de aguacate siguió el mismo patrón de distribución a través de las estaciones del año. La tasa de A al fin de verano fue baja (18.45 mmol CO2 m-2 s-1, se elevó ligeramente (20.22 mmol CO2 m-2 s-1 al inicio de otoño, y alcanzó el promedio máximo en pleno otoño con 30.05 mmol CO2 m-2 s-1 que coincidió con el crecimiento de fruto. A se estabilizó (29.84 mmol CO2 m-2 s-1 durante el invierno y descendió significativamente al inicio de primavera (17.61 mmol CO2 m-2 s-1. La tasa de A resultó afectada por las condiciones climatológicas de cada estación y el estado fenológico de los cultivares de aguacate.

  1. Persea schiedeana (Lauraceae), nuevo hospedero de Heilipus lauri Boheman (Coleoptera: Curculionidae) en Veracruz, México

    OpenAIRE

    Castañeda-Vildózola, Alvaro; Del Ángel-Coronel, Oscar A; Cruz-Castillo, Juan G; Váldez-Carrasco, Jorge

    2009-01-01

    Se reporta por primera vez la presencia del Molytinae Heilipus lauri Boheman atacando frutos de Persea schiedeana, comúnmente conocidos como "chinene" in Hustusco y Zongolica, Veracruz, México. The presence of the Molytinae Heilipus lauri Boheman is reported for the first time attacking fruits of Persea schiedeana, commonly called "chinene" in Huatusco and Zongolica, state of Veracruz, Mexico.

  2. Antioxidant neolignans from Cordia americana.

    Science.gov (United States)

    Fernández, Lucía R; Cirigliano, Adriana; Fabani, María P; Lima, Beatriz; Alberti, Sebastián; Kramer, Fernando; Tapia, Alejandro A; Cabrera, Gabriela; Palermo, Jorge A; Sánchez, Marianela

    2013-12-01

    Five new neolignans with a bicyclo[2.2.2]octene framework were isolated from an ethanolic extract of the bark of Cordia americana. The structures and relative configurations of the compounds were elucidated by a combination of spectroscopic methods. All the isolated compounds showed good antioxidant activities in the DPPH radical scavenging (0.5-100 µg/mL) and Ferric-reducing antioxidant power (FRAP, 1-100 µg/mL) assays. One of the compounds displayed mild fungistatic activity at 0.1 µmol/spot against Fusarium virguliforme while, at the same time, all compounds were inactive against several strains of Gram (+) and Gram (-) bacteria at all assayed concentrations (10-1,000 µg/mL). Georg Thieme Verlag KG Stuttgart · New York.

  3. Dynamics of the regional groundwater flow in the Jaruco - Aguacate Karst system, Cuba; Dinamica del flujo regional en el sistema Carsico Jaruco-Aguacate, Cuba

    Energy Technology Data Exchange (ETDEWEB)

    Molerio Leon, L F; Guerra Oliva, M G; Regalado, O A; Arellano Acosta, D M; March Delgado, C [Instituto Nacional de Recursos Hidraulicos, La Habana (Cuba). Centro de Hidrologia y Calidad de las Aguas; Maloszweski, P [Instituto de Hidrologia, GSF, Oberschlessheim (Germany); Del Rosario, K [Instituto Nacional de Recursos Hidraulicos, Matanzas (Cuba). Delegacion Provincial Matanzas

    1995-10-01

    The hydrochemical and isotopic investigations of the Jaruco-Aguacate system have provided information on the location of the water divide between the two subsystems and on the causes for its spatial variability, as well as the consequences of such changes in the volume and quality of groundwater in every subsystem. Moreover, unexpected results were obtained with regard to the vertical stratification of every subsystem. After deducting the existence of two aquifer horizons, it was possible to formulate a more precise mathematical model for simulating the processes of mass transport and increase our knowledge of the water balance of the whole system. The water balance showed that the aquifer is overexploited, with a deficit of 1.5 m{sup 3}/s, or referring to the infiltration rate, a deficit of 70 mm/a. The assumed fissure porosity (5%) represents a lowering of the water table of 1.4 m/a, assuming a constant infiltration rate. However, in 1992, the precipitation was 33% lower than the long term mean, and it is estimated that the infiltration rate was only 170 mm, compared to a mean value of 200 mm/a. The natural flow system was totally altered by the pumping of the El Gatto well field. Since the beginning of the pumping, the water table dropped by around 10 m, which is in accordance with the results. (author). 30 refs, 20 figs, 9 tabs.

  4. Evaluation gives productivity and quality gives fruit in Aguacate subjected has to gamma radiation

    International Nuclear Information System (INIS)

    De La Cruz Torres, Eulogio; Garcia Andrade, Juan M.; Ibannez Palacios, Jorge; Mijares Oviedo, Pedro

    1999-01-01

    Evaluation of productivity, post harvest behavior and fruit quality was performed on four years Has avocado trees irradiated with 60 Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the La Labor Experimental Center of the Centro de Investigaciones Cientificas y Tecnologicas del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300%. In regard to post harvest performance, the respiration index (mg CO2 /kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This means that radiation has altered productivity but not the quality and post harvest behavior of fruits

  5. Multi-drug resistant Ewingella Americana

    International Nuclear Information System (INIS)

    Bukhari, Syed Z.; Ashshi, Ahmad M.; Hussain, Waleed M.; Fatani, Mohammad I.

    2008-01-01

    We report a case of pneumonia due to multi-drug resistant Ewingella Americana in a young patient admitted in the Intensive Care Unit of Hera General Hospital, Makkah, Saudi Arabia with severe head injury in a road traffic accident. He was an Indonesian pilgrim who had traveled to the Kingdom of Saudi Arabia to perform Hajj in December 2007. Ewingella Americana was identified to be the pathogen of pneumonia with clinical signs and symptoms along with positive radiological findings. (author)

  6. O óleo de abacate (Persea americana Mill como matéria-prima para a indústria alimentícia The avocado oil (Persea americana Mill as a raw material for the food industry

    Directory of Open Access Journals (Sweden)

    Jocelem Mastrodi Salgado

    2008-12-01

    Full Text Available Hábitos de vida saudáveis e uma dieta balanceada aliados ao alto consumo de frutas e vegetais estão associados à redução do risco de doenças e à manutenção da saúde. O óleo de abacate possui em sua composição substâncias bioativas capazes de prevenir e controlar as dislipidemias. Como existem poucas pesquisas científicas avaliando o potencial deste óleo para o consumo humano, o presente trabalho estudou os processos de extração e refino do óleo de abacate. Para tanto, procedeu-se à separação da polpa das outras partes da fruta. A polpa fresca foi seca em estufa, e posteriormente moída para a obtenção de um farelo. O óleo obtido do farelo foi extraído e caracterizado. Os resultados mostraram que os processos de extração e refino do óleo a partir da variedade Margarida são tecnicamente viáveis, o que o torna excelente matéria-prima para a indústria alimentícia. Além disso, possui um perfil de ácidos graxos e esteróis muito semelhante ao perfil do azeite de oliva, podendo desta forma, substituir o óleo de soja e ser utilizado juntamente com o azeite de oliva nos óleos mistos, oferecendo ao consumidor brasileiro um produto de qualidade superior e com menor custo.Healthy life habits and an adequate diet, together with a high intake of fruits and vegetables, are associated with reducing the risk of diseases and maintaining health. The avocado oil has bioactive substances that can prevent and control the hyperlipidemia. Since there is little scientific research evaluating the oil potential for human consumption, the present work studied the extraction and refining process of the avocado oil. Firstly, the pulp was extracted from the fruit. The fresh pulp was dried in a stove and then grounded for obtaining the bran. The oil obtained from de bran was extracted and characterized. The results showed that the extraction and refining process of the Margarida avocado variety is technically feasible, and it is an excellent raw material for the food industry. Besides, the fatty acid composition and the sterols presents in the avocado oil are similar to the olive oil. Thus, the avocado oil can substitute the soybean oil, or it can be mixed with the olive oil offering the Brazilian consumers a product of high quality and low cost.

  7. Chemical composition, toxicity and larvicidal and antifungal activities of Persea americana (avocado seed extracts Composição química, toxicidade, atividade larvicida e antifúngica de extratos de semente de Persea americana (abacate

    Directory of Open Access Journals (Sweden)

    João Jaime Giffoni Leite

    2009-04-01

    Full Text Available The present study had the aim of testing the hexane and methanol extracts of avocado seeds, in order to determine their toxicity towards Artemia salina, evaluate their larvicidal activity towards Aedes aegypti and investigate their in vitro antifungal potential against strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis through the microdilution technique. In toxicity tests on Artemia salina, the hexane and methanol extracts from avocado seeds showed LC50 values of 2.37 and 24.13mg mL-1 respectively. Against Aedes aegypti larvae, the LC50 results obtained were 16.7mg mL-1 for hexane extract and 8.87mg mL-1 for methanol extract from avocado seeds. The extracts tested were also active against all the yeast strains tested in vitro, with differing results such that the minimum inhibitory concentration of the hexane extract ranged from 0.625 to 1.25mg L-¹, from 0.312 to 0.625mg mL-1 and from 0.031 to 0.625mg mL-1, for the strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis, respectively. The minimal inhibitory concentration for the methanol extract ranged from 0.125 to 0.625mg mL-1, from 0.08 to 0.156mg mL-1 and from 0.312 to 0.625mg mL-1, for the strains of Candida spp., Cryptococcus neoformans and Malassezia pachydermatis, respectively.O presente estudo teve como objetivo testar os extratos hexânico e metanólico das sementes do abacate, a fim de determinar sua toxicidade em Artemia salina, avaliar a atividade larvicida frente ao Aedes aegypti, bem como verificar o potencial antifúngico in vitro contra cepas de Candida spp, Cryptococcus neoformans e Malassezia pachydermatis, através da técnica de microdiluição. Os extratos hexânico e metanólico das sementes de abacate apresentaram no teste de toxicidade frente à Artemia salina, valores de LC50 2,37 e 24,13mg L-1, respectivamente; contra as larvas do Aedes aegypti os resultados obtidos foram LC50 16,7mg L-1 para o extrato hexânico e 8,87mg L-1 para o extrato metanólico das sementes do abacate. Os extratos testados também foram ativos contra todas as cepas de leveduras, testadas in vitro, apresentando diferentes resultados, onde o MIC do extrato hexânico variou de 0,625 a 1,25mg mL-1, de 0,312 a 0,625mg mL-1 e de 0,031 a 0,625mg mL-1 para as cepas de Candida spp., Cryptococcus neoformans e Malassezia pachydermatis, respectivamente. O intervalo de MIC para o extrato metanólico foi de 0,125 a 0,625mg mL-1, 0,08 a 0,156mg mL-1 e de 0,312 a 0,625mg mL-1, para as exemplares de Candida spp., Cryptococcus neoformans e Malassezia pachydermatis, respectivamente.

  8. Use of avocado peel (Persea americana in tea formulation: a functional product containing phenolic compounds with antioxidant activity

    Directory of Open Access Journals (Sweden)

    Eliza Mariane Rotta

    2015-01-01

    Full Text Available The peels of avocados, like other fruit peels, are commonly discarded, not knowing their potential use. In order to reuse avocado peel, the chemical and mineral compositions, total phenolic and flavonoid contents as well as antioxidant activities have been investigated by DPPH (1,1-diphenyl-2-picrylhydrazyl and FRAP(ferric-reducing antioxidant power methods in in natura and dehydrated avocado peel. Dehydrated avocado-peel tea was manufactured and the antioxidant activity was evaluated, as well as their flavonoid and phenolic compound contents, and compared with other teas marketed. Avocado peel, especially dried avocado peel, contains major phenolic compounds (10,848.27 ± 162.34 mg GAE kg-1 and flavonoids (1,360.34 ± 188.65 mg EQ kg-1. The avocado-peel tea showed antioxidant activity by DPPH (1,954.24 ± 87.92 e 2518.27 ± 192.59 mg TE L-1 and phenolic and flavonoids contents highest than apple tea. The avocado-peel tea showed good antioxidant activity and had good acceptability by sensory analysis as a promising product.

  9. Somatic embryogenesis and embryo culture coupled with gamma irradiation for generating avocado (Persea americana Miller) mutants in the Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Avenido, R. A. [Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños (Philippines); Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (Philippines); Galvez, H. F.; Dimaculangan, J. G.; Welgas, J. N.; Frankie, R. B.; Damasco, O. P. [Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños (Philippines)

    2009-05-15

    Plant regeneration through somatic embryogenesis from immature zygotic embryos and embryo cultures from mature fruits were achieved in select avocado accession ‘Semil’ and other seedling trees in the Philippines. Embryogenic cultures were induced from immature zygotic embryos of eight (8) avocado genotypes using either SE1 medium (MS + 30 g/l sucrose + 5 mg/l 2, 4-D + 0.5 mg/l BAP) or SE2 medium (MS + 30 g/l sucrose + 0.1 mg/l picloram). Embryogenic cultures of 2 genotypes namely ‘Semil’ and ‘Mainit’ developed into somatic embryos after repeated subcultures in SE2, SE3 (MS + 30 g/l sucrose + 0.1 mg/l TDZ + 0.5 mg/l GA{sub 3}) and SE4 (MS + 30 g/l sucrose + 2 mg/l BAP + 1 mg/l IBA) media. Plant/shoot regeneration from ‘Semil’ somatic embryos was recorded in 3 trials at 16.3, 23.0 and 20.7%, and was affected by culture age, light treatment and media used. R4 regeneration medium (B5 macro salts + MS minor salts and vitamins + 60 g/l sucrose + 400 g/l glu + 2 mg/l BAP + 4.5 g/l Phytagel was found to be the best. Gamma irradiation (10 to 30 Gy) of embryogenic cultures of ‘Semil’ resulted in reduced proliferation and formation of cotyledonary stage somatic embryos. However, shoot regeneration from somatic embryos from gamma-irradiated cultures was comparable or even higher (17.8 to 26.9%) as compared to the control (18.3%). Over 200 somatic embryo-derived putative variant/mutant lines from tissue culture and gamma irradiation experiments are being maintained as shoot cultures. Due to slow growth and other related problems, micrografting and in vitro rooting were used to rescue and ensure the greenhouse establishment of putative mutant shoots, and fast-track mutant confirmation by genetic analysis. Preliminary genetic analyses by SSR revealed that (a) the 3 asexually propagated ‘Semil’ mother trees are genetically similar, and (b) mutations marked by the generation of a new allele (band) at the SSR locus was evident among the somatic embryo-derived regenerants from non-irradiated embryogenic cultures. Mass screening for mutations will be done on all successfully potted out regenerants. A second protocol involving embryo culture and gamma irradiation was also done. Mature embryos of 9 genotypes were cultured using liquid MS basal medium with 30 g/l sucrose and 2 mg/l BAP, with 100% germination. Germinating zygotic embryos of ‘San Felix’ were also gamma irradiated and the LD{sub 50} between 20 to 30 Gy was established. M{sub 1}V{sub 4} shoots were generated from gamma-irradiated seedlings after subsequent micropropagation cycles. This is the first successful application of tissue culture and gamma irradiation technologies towards the improvement of a woody perennial fruit crop in the Philippines. (author)

  10. DNA polymorphisms in cuban varieties of avocado (persea americana mill.) as detected by inverse sequence tagged repeat (ISTR) analysis

    Energy Technology Data Exchange (ETDEWEB)

    Ramirez, M; Fuentes, J L [Centro de Estudios Aplicados al Desarrollo Nuclear, La Habana (Cuba); Rodriguez, N N; Cueto, J [Max Planck Institut fur Zuchtungsforschung (MPIZ), Koln (Germany); Becker, D; Rohde, W [Instituto de Investigaciones en Fruticultura Tropical (IIFT). C. Habana (Cuba)

    2001-07-01

    A survey of the genetic diversity among commercial Cuban avocado varieties was initiated using ISTR analysis. ISTR markers were efficient in detecting polymorphisms among the genotypes. The obtained dissimilarities values ranged from 0.24 between var. Suardia and Hass to 1.00 between Lula and Los Moros or CHI-3 with an average dissimilarity of 0.78. A cluster analysis was performed based on dissimilarity using UPGMA as the clustering method. The efficiency of UPGMA in estimating genetic relationships between varieties was corroborated by the cophenetic correlation coefficients, which indicated that the distortion degree in the relationship of the estimated dissimilarities was minimal. Ecological groups were not adequately represented in the dendrogram. Thus, West Indians, Guatemalan and Mexican genotypes were positioned across the dendrogram. The utility of ISTR for genotype identification and assessment of genetic diversity in commercial avocado varieties is discussed.

  11. DNA polymorphisms in cuban varieties of avocado (persea americana mill.) as detected by inverse sequence tagged repeat (ISTR) analysis

    International Nuclear Information System (INIS)

    Ramirez, M; Fuentes, J. L.; Rodriguez, N.N.; Cueto, J.; Becker, D.; Rohde, W.

    2001-01-01

    A survey of the genetic diversity among commercial Cuban avocado varieties was initiated using ISTR analysis. ISTR markers were efficient in detecting polymorphisms among the genotypes. The obtained dissimilarities values ranged from 0.24 between var. Suardia and Hass to 1.00 between Lula and Los Moros or CHI-3 with an average dissimilarity of 0.78. A cluster analysis was performed based on dissimilarity using UPGMA as the clustering method. The efficiency of UPGMA in estimating genetic relationships between varieties was corroborated by the cophenetic correlation coefficients, which indicated that the distortion degree in the relationship of the estimated dissimilarities was minimal. Ecological groups were not adequately represented in the dendrogram. Thus, West Indians, Guatemalan and Mexican genotypes were positioned across the dendrogram. The utility of ISTR for genotype identification and assessment of genetic diversity in commercial avocado varieties is discussed

  12. Identification of avocado (Persea americana) root proteins induced by infection with the oomycete Phytophthora cinnamomi using a proteomic approach.

    Science.gov (United States)

    Acosta-Muñiz, Carlos H; Escobar-Tovar, Lina; Valdes-Rodríguez, Silvia; Fernández-Pavia, Silvia; Arias-Saucedo, Luis J; de la Cruz Espindola Barquera, Maria; Gómez Lim, Miguel Á

    2012-01-01

    Avocado root rot, caused by Phytophthora cinnamomi, is the most important disease that limits avocado production. A proteomic approach was employed to identify proteins that are upregulated by infection with P. cinnamomi. Different proteins were shown to be differentially expressed after challenge with the pathogen by two-dimensional (2-D) gel electrophoresis. A densitometric evaluation of protein expression indicated differential regulation during the time-course analyzed. Some proteins induced in response to the infection were identified by standard peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry and sequencing by MALDI LIFT-TOF/TOF tandem mass spectrometry. Of the 400 protein spots detected on 2-D gels, 21 seemed to change in abundance by 3 hours after infection. Sixteen proteins were upregulated, 5 of these were only detected in infected roots and 11 showed an increased abundance. Among the differentially expressed proteins identified are homologs to isoflavone reductase, glutathione S-transferase, several abscisic acid stress-ripening proteins, cinnamyl alcohol dehydrogenase, cinnamoyl-CoA reductase, cysteine synthase and quinone reductase. A 17.3-kDa small heat-shock protein and a glycine-rich RNA-binding protein were identified as downregulated. Our group is the first to report on gene induction in response to oomycete infection in roots from avocado, using proteomic techniques. Copyright © Physiologia Plantarum 2011.

  13. Isolation and structure elucidation of avocado seed (Persea americana) lipid derivatives that inhibit Clostridium sporogenes endospore germination.

    Science.gov (United States)

    Rodríguez-Sánchez, Dariana Graciela; Pacheco, Adriana; García-Cruz, María Isabel; Gutiérrez-Uribe, Janet Alejandra; Benavides-Lozano, Jorge Alejandro; Hernández-Brenes, Carmen

    2013-07-31

    Avocado fruit extracts are known to exhibit antimicrobial properties. However, the effects on bacterial endospores and the identity of antimicrobial compounds have not been fully elucidated. In this study, avocado seed extracts were tested against Clostridium sporogenes vegetative cells and active endospores. Bioassay-guided purification of a crude extract based on inhibitory properties linked antimicrobial action to six lipid derivatives from the family of acetogenin compounds. Two new structures and four compounds known to exist in nature were identified as responsible for the activity. Structurally, most potent molecules shared features of an acetyl moiety and a trans-enone group. All extracts produced inhibition zones on vegetative cells and active endospores. Minimum inhibitory concentrations (MIC) of isolated molecules ranged from 7.8 to 15.6 μg/mL, and bactericidal effects were observed for an enriched fraction at 19.5 μg/mL. Identified molecules showed potential as natural alternatives to additives and antibiotics used by the food and pharmaceutical industries to inhibit Gram-positive spore-forming bacteria.

  14. Description of morphologic characters in avocado trees (Persea americana. Mill) var. Hass, submitted to gamma radiation of 60 Co

    International Nuclear Information System (INIS)

    Sandoval R, L.

    2004-01-01

    It was carried out the characterization of 77 trees of avocado ''Hass'' subjected to gamma radiation of Co 60 , in doses of 0, 5, 10,15, 20 and 25 Gy based on previous studies of radiosensitivity with ends of improvement located in the experimental center ''La Labor'' belonging to the foundation Salvador Sanchez Colin. CICTAMEX. S C. in Temascaltepec, Mexico. It was carried out the morphological characterization of this variety according to the nomenclature settled down by the International Office of Genetic Resources (IBPGRI), registering 27 variables for the description of the tree, 33 for description of the fruit and 14 for the characterization of the seed. Regarding the architectural characterization 10 variables were evaluated. Using gamma radiation of Co 60 in the doses of 15 and 20 Gy it was detected a reduction of 22% in the portage of the tree, likewise had reduction the diameter of the trunk and of the foliage. It was found that the reduction in the behavior of the tree induces to a bigger variation of the form of the same one, in the distribution of the branches in the form of the fruit and in the form of the seed you Also detects a remarkable increment in the productivity of some hoist irradiated regarding the architecture of the tree one observes that the angle of inclination of the branches increase when being increased the radiation dose and that the longitude of the annual section of growth decreased significantly in the doses of 20 and 25 Gy. One observes a significant Increment in the I number of buds prolepticos in those hoist irradiated in the I finish mbdulo of rhythmic growth. Io that indicates a change substantial in the I inhabit of growth With the purpose of determining if the changes in morphology and architecture of the tree corresponded with the modification at level meristematico, they were carried out observations to the court microscope of having knitted apical vegetative meristematico in samples of you hoist of all the treatments including the witness. without detecting significant modifications in disposition and tamario of the cells of the tunic and the body of the bud veqetativo

  15. Inhibition of cholesterol oxidation products (COPs) formation in emulsified porcine patties by phenolic-rich avocado (Persea americana Mill.) extracts.

    Science.gov (United States)

    Rodríguez-Carpena, Javier-Germán; Morcuende, David; Petrón, María Jesus; Estévez, Mario

    2012-03-07

    The effect of phenolic-rich extracts from avocado peel on the formation of cholesterol oxidation products (COPs) in porcine patties subjected to cooking and chill storage was studied. Eight COPs (7α-hydroxycholesterol, 7β-hydroxycholesterol, 7-ketocholesterol, 20α-hydroxycholesterol, 25-hydroxycholesterol, cholestanetriol, 5,6β-epoxycholesterol, and 5,6α-epoxycholesterol) were identified and quantified by GC-MS. The addition of avocado extracts (∼600 GAE/kg patty) to patties significantly inhibited the formation of COPs during cooking. Cooked control (C) patties contained a larger variety and greater amounts of COPs than the avocado-treated (T) counterparts. COPs sharply increased in cooked patties during the subsequent chilled storage. This increase was significantly higher in C patties than in the T patties. Interestingly, the amount of COPs in cooked and chilled T patties was similar to those found in cooked C patties. The mechanisms implicated in cholesterol oxidation in a processed meat product, the protective effect of avocado phenolics, and the potential implication of lipid and protein oxidation are thoroughly described in the present paper.

  16. Fatty acid profile and elemental content of avocado (Persea americana Mill.) oil--effect of extraction methods.

    Science.gov (United States)

    Reddy, Mageshni; Moodley, Roshila; Jonnalagadda, Sreekanth B

    2012-01-01

    Interest in vegetable oil extracted from idioblast cells of avocado fruit is growing. In this study, five extraction methods to produce avocado oil have been compared: traditional solvent extraction using a Soxhlet or ultrasound, Soxhlet extraction combined with microwave or ultra-turrax treatment and supercritical fluid extraction (SFE). Traditional Soxhlet extraction produced the most reproducible results, 64.76 ± 0.24 g oil/100 g dry weight (DW) and 63.67 ± 0.20 g oil/100 g DW for Hass and Fuerte varieties, respectively. Microwave extraction gave the highest yield of oil (69.94%) from the Hass variety. Oils from microwave extraction had the highest fatty acid content; oils from SFE had wider range of fatty acids. Oils from Fuerte variety had a higher monounsaturated: saturated FA ratio (3.45-3.70). SFE and microwave extraction produced the best quality oil, better than traditional Soxhlet extraction, with the least amount of oxidizing metals present. Copyright © Taylor & Francis Group, LLC

  17. Effect of an avocado oil-enhanced diet (Persea americana) on sucrose-induced insulin resistance in Wistar rats.

    Science.gov (United States)

    Del Toro-Equihua, Mario; Velasco-Rodríguez, Raymundo; López-Ascencio, Raúl; Vásquez, Clemente

    2016-04-01

    A number of studies have been conducted to evaluate the effects of vegetable oils with varying percentages of monounsaturated and polyunsaturated fatty acids on insulin resistance. However, there is no report on the effect of avocado oil on this pathologic condition. The aim of this work was to evaluate the effect of avocado oil on sucrose-induced insulin resistance in Wistar rats. An experimental study was carried out on Wistar rats that were randomly assigned into six groups. Each group received a different diet over an 8-week period (n = 11 in each group): the control group was given a standard diet, and the other five groups were given the standard feed plus sucrose with the addition of avocado oil at 0%, 5%, 10%, 20%, and 30%, respectively. Variables were compared using Student t test and analysis of variance. Statistically significant difference was considered when p avocado oil showed lower insulin resistance (p = 0.022 and p = 0.024, respectively). Similar insulin resistance responses were observed in the control and 30% avocado oil addition groups (p = 0.85). Addition of 5-30% avocado oil lowered high sucrose diet-induced body weight gain in Wistar rats. It was thus concluded that glucose tolerance and insulin resistance induced by high sucrose diet in Wistar rats can be reduced by the dietary addition of 5-20% avocado oil. Copyright © 2016. Published by Elsevier B.V.

  18. KANDUNGAN GIZI, RENDEMEN TEPUNG, DAN KADAR FENOL TOTAL ALPUKAT (Persea americana, Mill VARIETAS I JO PANJANG DAN I JO BUNDAR

    Directory of Open Access Journals (Sweden)

    Wuri Marsigit

    2016-04-01

    Full Text Available The aims of the study were to determine pulp proportion, dried pulp rendement, nutrient dan  total phenolic content avocado variety of Ijo Panjang dan Ijo Bundar. Variety of Ijo Panjang and Ijo Bundar were selected because both of varieties were categorized as excelent varities and recomendedto develop in Indonesia. Water, carbohydrate, protein, fat dan ash content were determined by using proxymate analysis (AOAC, vitamin A dan E using spectrophotometry method, vitamin C using titration method, dan minerals using AAS method. Total phenolic content were determined by using Follin-Cialcetau method. The result of the studies found that pulp portion Ijo Bundar Variety higher than Ijo Panjang. Water content dan zinc of Ijo Panjang variety higher than Ijo Bundar. Dried pulp rendement of Ijo Bundar higher than Ijo Panjang. Protein, fat,  ash, minerals (Fe, Na, K dan P avocado variety of Ijo Bundar higher than Ijo Panjang. Magnesium dan mangan content of both varieties have not significant different. Total phenolic content of pulp dan dried pulp were higher in Ijo Bundar than Ijo Bundar. Keywords: Pulp proportion, dried pulp rendement, nutrients dan total phenolics content   ABSTRAK Penelitian ini bertujuan untuk mengetahui proporsi daging buah, rendemen tepung, kandungan gizi dan fenol total alpukat varietas Ijo Panjang dan Ijo Bundar. Pemilihan kedua varietas tersebut karena merupakan varietas unggul dan direkomendasikan untuk dikembangkan di Indonesia. Kandungan, air, karbohidat, protein, lemak dan abu dilakukan dengan analisis proksimat (AOAC, kandungan vitamin A dan E dengan metode spektrofotometri dan vitamin C dengan metode titrasi, analisis mineral dengan metode AAS. Total fenol dianalisis dengan metode Folin-Ciocalteu. Hasil penelitian menunjukan bahwa proporsi daging buah, kadar air, seng varietas Ijo Panjang lebih tinggi dibandingkan Ijo Bundar. Rendemen tepung alpukat lebih tinggi pada varietas Ijo Bundar. Kandungan protein, lemak, abu, vitamin (A,E dan C, mineral (Fe, Na, K, P lebih tinggi pada varietas Ijo Bundar. Kandungan magnesium dan mangan tidak terdapat perbedaan signifikan kedua varietas.  Fenol total daging buah dan tepung alpukat lebih tinggi pada varietas Ijo Bundar dibandingkan Ijo Panjang. Kata kunci: Prorporsi daging buah, rendemen tepung, kandungan gizi, fenol total

  19. Description of a new species of Pleseobyrsa (Heteroptera: Tingidae from Costa Rica Descripción de una nueva especie de Pleseobyrsa (Heteroptera: Tingidae de Costa Rica

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    Sara I. Montemayor

    2011-06-01

    Full Text Available A new Pleseobyrsa, P. persea n. sp. is described for Costa Rica. The host plant of this new species is Persea americana P. Mill. This corresponds to the second species of the genus known for the country and the third whose host plant is P. americana (avocado. A description of the adult as well as of instar V are provided, together with a key to species, photographs taken in the field, and illustrations of the fifth nymph stage, adult, and the main characters.Se describe una especie nueva de Pleseobyrsa, P. persea n. sp. de Costa Rica, cuya planta huésped es Persea americana P. Mill., siendo ésta la segunda especie del género conocida del país y la tercera cuya planta huésped es P. americana (aguacate. Se describen tanto el adulto como el estadio ninfal V; se agrega una clave modificada de Froeschner para las especies, y se ilustran ejemplares en el campo, el quinto estadio ninfal, adulto y los principales caracteres.

  20. Factores que limitan la posibilidad de elevar la eficiencia de las organizaciones productoras de aguacate: el caso del municipio de Tetela del Volcán, Morelos

    Directory of Open Access Journals (Sweden)

    Benjamín Bazaldúa Muñoz

    2014-01-01

    Full Text Available El objetivo fundamental de este trabajo es describir y analizar algunos de los factores que influyen en la baja producti - vidad de las organizaciones productoras de aguacate, particularmente las que se encuentran localizadas en Tetela del Volcán, estado de Morelos. Las preguntas centrales que guían está investigación son las siguientes: ¿cuáles son algunos de los principales factores que limitan o influyen en el incremento de la eficiencia de las organizaciones empresariales desde el punto de vista teórico y empírico?, ¿cuáles son los niveles de producción y de eficiencia productiva del aguacate que se registran a nivel mundial, estatal y municipal?, y ¿de qué manera la tecnología y el capital humano influyen en la eficiencia de los productores de aguacate?

  1. Cristian-Vanessa un cultivar de aguacate tardío para las condiciones de Cuba

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    Rafael Jiménez Villasuso

    2015-06-01

    Full Text Available RESUMEN El trabajo se llevó acabo en la Unidad Científico Tecnológica de Base (UCTB Alquízar, adscripta al Instituto de Investigaciones en Fruticultura Tropical (IIFT, ubicada en la provincia de Artemisa, situada a los 22°77'45'' de latitud Norte y a los 82°56'28'' de longitud Oeste y a 6.80 m. s. n. m. Se realizó un estudio sobre 100 árboles de aguacate de diferentes grupos ecológicos propagados por semillas durante 7 años, teniendo en cuenta como variables principales: porte del árbol, tamaño de los frutos, época de cosecha y nivel de producción, plantados sobre un suelo Ferrasol Rhodic. Con los resultados de esta evaluación se seleccionó este cultivar, posteriormente se injertó sobre árboles a los que se le realizó el cambio de copas en el Banco de Yemas de UCTB y luego evaluar su comportamiento, mediante el descriptor IPGRI para el cultivo del aguacate, evaluándose 20 variables cuantitativas y 30 cualitativas de los frutos, además se determinó el crecimiento y la producción en kg/árbol en tres años de cosecha. Con los resultados obtenidos, se establecieron 25 árboles en el 2010 en la práctica productiva, en tres localidades del país: Güines, Pinar del Río y Jagüey Grande para determinar su comportamiento. El objetivo de este trabajo consistió en caracterizar y proponer un cultivar tardío de aguacate, para suplir los meses de enero-febrero, con lo cual se alargaría el período de cosecha de este frutal en el país y que al mismo tiempo reúna las exigencias de consumo nacional y para la exportación. Cristian-Vanessa a cultivar of late avocado for the Cuba conditions ABSTRACT The work was carry out in the Scientific Technological of Base Unit (UCTB Alquízar, of the Research Institute of Investigations in Tropical Fruit (IIFT, located in the province of Artemisa, put to the 22°77 " 45 "" of north latitude and to the 82°56 " 28 "" of west longitude and to 6.80 m. s. n. m. It carried out to him a study on 100

  2. CORRECCIÓN DE LA DEFICIENCIA CRÓNICA DE ZINC EN AGUACATE "HASS"

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    S. Salazar-García

    2008-01-01

    Full Text Available En los huertos de aguacate "Hass" de los municipios de Tepic y Xalisco, Nayarit, son frecuentes los niveles foliares debajo de lo normal de zinc (Zn y la presencia de síntomas visuales de deficiencia de Zn en hojas, brotes y frutos. Esta investigación se desarrolló del 2001 al 2005 en dos huertos comerciales de aguacate ''Hass'' cultivados sin riego en el municipio de Tepic con el objetivo de evaluar el efecto de las aplicaciones de sulfato de zinc (ZnSO4, al follaje o al suelo, sobre los niveles foliares de Zn, así como sobre la producción, tamaño y forma del fruto. El suelo de los huertos era de textura ligera, pH 5.8 y bajo contenido de Zn (1.4 a 3.13 mg.kg- 1. Los tratamientos al follaje fueron aplicados en 8 L agua/árbol y consistieron en: a una aspersión con 4,056 g ZnSO4.L-1 agua (1.46 g Zn, y b dos aspersiones con 2.28 g ZnSO4.L-1 agua (0.73 g Zn; ambos tratamientos proporcionaron 11.68 g Zn.árbol-1. Los tratamientos al suelo consistieron en: a una aplicación (1.5 kg, y b dos aplicaciones (0.75 kg c/u anuales de ZnSO4 (35.5 % Zn al suelo. El tratamiento control no recibió Zn. Las aspersiones foliares con ZnSO4 no corrigieron la deficiencia foliar de Zn, ni tuvieron efecto sobre la producción y tamaño del fruto. Dos aplicaciones al suelo con 0.75 kg ZnSO4.árbol-1.año resultaron en el mayor incremento del rendimiento, tamaño y forma (más alargada del fruto, reduciendo la cantidad de frutos pequeños y redondos. Este tratamiento también incrementó los niveles de Zn en las hojas y en la pulpa del fruto. Se encontró una pobre relación entre los niveles de ZnSO4 aplicados al suelo y el contenido foliar de Zn.

  3. Diversidad de la herpetofauna en huertos de aguacate y hábitats conservados en Michoacán, México

    OpenAIRE

    Marroquín-Páramo, Jorge Alejandro; Suazo-Ortuño, Ireri; Mendoza, Eduardo; Alvarado-Díaz, Javier; Siliceo-Cantero, Héctor Hugo

    2017-01-01

    Resumen: La conversión de los hábitats naturales en monocultivos es un fenómeno que se presenta cada vez con mayor frecuencia, por lo que es necesario el estudio de su impacto sobre la diversidad, por ejemplo, de la herpetofauna. En el presente estudio se realizó un muestreo intensivo de anfibios y reptiles en 3 sitios en huertas de aguacate, 3 en bosque de coníferas (vegetación de la que derivan las huertas de aguacate) y 3 en bosque tropical caducifolio (usado como punto de referencia al es...

  4. Iridoids from leaf extract of Genipa americana

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    Jovelina S.F. Alves

    Full Text Available Abstract Genipa americana L., Rubiaceae, is a plant native from Brazil popularly known as “jenipapo”. Two iridoids, 1-hydroxy-7-(hydroxymethyl-1,4aH,5H,7aH-cyclopenta[c]pyran-4-carbaldehyde (1, and iridoid 7-(hydroxymethyl-1-methoxy-1H,4aH,5H,7aH-cyclopenta[c]pyran-4-carbaldehyde (2 were isolated and identified in the leaf extract of G. americana. Compounds 1 and 2 were identified for the first time in G. americana, and 1 has not been yet described in literature. These substances were analyzed by spectroscopic techniques such as infrared, high resolution mass spectrometry, 1H and 13C 1D; as well as 2D nuclear magnetic resonance. Moreover, the presence of flavonoids was detected by a preliminary analysis by Thin Layer Chromatography.

  5. [Persea schiedeana (Lauraceae), a new host of Heilipus lauri Boheman (Coleoptera: Curculionidae) in Veracruz, Mexico].

    Science.gov (United States)

    Castañeda-Vildózola, Alvaro; Del Angel-Coronel, Oscar A; Cruz-Castillo, Juan G; Váldez-Carrasco, Jorge

    2009-01-01

    The presence of the Molytinae Heilipus lauri Boheman is reported for the first time attacking fruits of Persea schiedeana, commonly called 'chinene' in Huatusco and Zongolica, state of Veracruz, Mexico.

  6. VALIDACIÓN DE UN MÉTODO PARA EL ANÁLISIS DE RESIDUALIDAD DE PLAGUICIDAS EN AGUACATE HASS

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    SANTIAGO BENAVIDES

    2014-07-01

    Full Text Available El aguacate es una fruta que se caracteriza por su alto contenido de grasas, siendo la variedad Hass la de mayor cantidad de pigmentos y componentes fenólicos, lo que la convierte en una matriz compleja para el análisis de residuos de plaguicidas. Se validó un método para el análisis simultáneo de residualidad de tres plaguicidas en aguacate fresco de la variedad Hass. Se aplicó la técnica QuEChERS siguiendo una modificación del protocolo AOAC 2007.1; para la preparación de las muestras se empleó acetonitrilo acidificado, MgSO4 y CH3COONa y se limpiaron por extracción en fase solida dispersiva con una mixtura de sorbentes: amina primaria secundaria (PSA, octadecilo (C18 y carbón grafitado negro GCB. Los plaguicidas fueron analizados por GC-μECD. En el análisis de linealidad se obtuvieron correlaciones de 0,9908; 0,9968 y 0,9965 para dimetoato, malatión y deltametrina respectivamente y se cumplieron las pruebas de significancia. El efecto matriz provoca una mejora en la sensibilidad de la curva de calibración producto de la concentración de la muestra en la extracción. Se lograron límites de cuantificación de 0,02mg/kg para los tres compuestos con estimaciones de incertidumbre menores al 50%. Se cumplieron los criterios de precisión y exactitud, logrando una validación exitosa.

  7. First report of laurel wilt, caused by Raffaelea lauricola, on redbay (Persea borbonia) in Texas.

    Science.gov (United States)

    R. D. Menard; S. R. Clarke; Stephen Fraedrich; T. C. Harrington

    2016-01-01

    Laurel wilt, caused by Raffaelea lauricola T.C.Harr., Aghayeva, & Fraedrich, a fungal symbiont of the redbay ambrosia beetle (Xyleborus glabratus Eichhoff), is responsible for extensive mortality of redbay (Persea borbonia (L.) Spreng) and other Lauraceae native to the United States (Fraedrich et al. 2008). The beetle and fungus were introduced into the United...

  8. Towards the development of a laurel wilt screening program in redbay (Persea borbonia)

    Science.gov (United States)

    Marc Hughes; Jason Smith

    2012-01-01

    Laurel wilt is a highly destructive disease of redbay (Persea borbonia (L.) Spreng.) and other Lauraceous natives in the southeastern United States. The disease and associated vector, the redbay ambrosia beetle (Xyleborus glabratus), has spread through the United States coastal plain. The presence of surviving and...

  9. The classification of fatty acids of lipids from seeds of Persea ...

    African Journals Online (AJOL)

    PROMOTING ACCESS TO AFRICAN RESEARCH ... Gas liquid chromatographic analyses of Persea grattisima and ... as oil seeds and the fatty-acids of seed lipids could be potential sources of industrial oil. Keywords: Classification, fatty acids, GLC and Lipids. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  10. Pronghorn (Antilocapra americana) Reintroduction and Management Plan [DRAFT

    Data.gov (United States)

    Department of the Interior — Pronghorn (Antilocapra americana) have been a component of North America’s grasslands for millions of years and have thus created a niche for themselves in remaining...

  11. A Rússia Americana The American Russia

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    João Marcelo Ehlert Maia

    2005-08-01

    Full Text Available Este artigo procura analisar interpretações do Brasil assentadas na idéia de que seríamos uma "Rússia Americana". Para tanto, analiso as contribuições de Gilberto Freyre, Otávio Velho e Vicente Licínio Cardoso, e apresento brevemente o caso russo, a partir das obras clássicas de Franco Venturi e Isaiah Berlin. Argumento ao longo do texto que essa percepção de que o Brasil teria uma geografia social "russa" implica a produção de um americanismo específico, que encontra sua maior expressão no pensamento de Vicente Licínio Cardoso e em suas idéias que conjugam fordismo e arielismo.This article seeks to analyse Brazil's interpretations founded on the idea that we would be an "American Russia". in order to do so, I analyse the contributions of Gilberto Freyre, Otávio Velho and Vicente Licínio Cardoso, and I briefly present the russian case, using the works of Franco Venturi and Isaiah Berlin. I suggest that this perception implies the production of an especific type of americanism, wich finds its greater expression on the unusual combination between fordism and arielism made by Vicente Licínio.

  12. EFECTO DE LA ADICIÓN DE AGUACATE A LA ALIMENTACIÓN HABITUAL SOBRE LOS NIVELES DE LÍPIDOS EN PERSONAS CON DISLIPIDEMIA

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    Martha Díaz-Perilla

    2004-12-01

    Full Text Available El propósito fue determinar el efecto del consumo diario de aguacate, fuente de ácidos grasos monoinsaturados sobre los niveles de lípidos sanguíneos en personas con dislipidemia. La muestra estuvo conformada por 30 pacientes de la Clínica de los Andes de Tunja, entre los 30 y 65 años. Se evaluaron los datos antropométricos, la frecuencia de consumo de alimentos y el perfil lipídico. Se les suministró a los pacientes 250 g de aguacate al día por cuatro semanas. Se empleó la estadística descriptiva y la prueba T de hipótesis con un nivel de significancia p< 0.05. Durante las cuatro semanas el aumento de peso de los hombres fue de 2.7 K y de las mujeres de 2.3 K (p<0.05. El nivel de colesterol total disminuyó en 14% (p<0.05 y el de LDL en 16% (P<0.05; el valor de HDL aumentó en un 19% (P<0.05, y el de los TG en un 12% (p =0.037. El 27% de la muestra normalizó los niveles de CT, el 30% los de LDL, el 91% aumentó el nivel de HDL y el 20% aumentó los de TG. El índice de riesgo de presentar enfermedad cardiovascular (CT/ HDL disminuyó en 29%. Según el test de Framinghan, 4 pacientes presentan más de un 20% de riesgo a 10 años de presentar cardiopatía coronaria. Los resultados muestran que el aguacate como fuente de ácidos grasos monoinsaturados, puede ser utilizado dentro del plan de alimentación de pacientes con dislipidemia, incluyéndolo dentro del aporte energético para evitar así un aumento de peso por su alta densidad calórica. En conclusión, en la población estudiada se evidenció el efecto benéfico del consumo diario de aguacate en términos de disminución de los niveles de colesterol total y LDL yaumento de HDL, resultados que tienen implicaciones como medida preventiva de la enfermedad coronaria.

  13. Los pequeños productores de aguacate del Ejido y la Comunidad de San Francisco Peribán, Michoacán (México)

    OpenAIRE

    Cristina Steffen Riedemann; Flavia Echánove Huacuja

    2003-01-01

    Una reiterada recomendación que las instituciones públicas vinculadas al agro hacen a los campesinos, es que supriman de su patrón de cultivos los productos en que México no tiene ventajas comparativas como los granos y se reconviertan hacia aquellos que puedan colocar en el mercado obteniendo mayor rentabilidad: frutas y hortalizas. En el presente trabajo se analiza el caso de ejidatarios y comuneros, quienes desde hace algunas décadas han emprendido la producción de aguacate,...

  14. Variabilidad intraespecífica de cepas de Phytophthora palmivora Butler (Butler) aisladas de plantaciones de aguacatero (Persea americana Mill.) en Cuba

    OpenAIRE

    Machado, Maylen; Peña-Marey, Mabel; Alvarez, Alba; Díaz, Adriana; Zamora, Victoria; Coto, Orlando

    2013-01-01

    Se utilizaron en el estudio ocho cepas de Phytophthora palmivora Butler (Butler) obtenidas de jardines de aguacateros de la región occidental de Cuba, y se describieron los patrones de crecimientos de las colonias en medio agar papa dextrosa (PDA), agar zanahoria (AZ), agar jugo vegetal V8 (AV8) y agar malta (AM), así como los tipos de esporangios presentes. Se evaluó la variabilidad intraespecífica de los aislamientos en base a las dimensiones de los esporangios y de sus papilas. Las cepas m...

  15. Effect of aqueous extracts of alligator pear seed (Persea americana mill) on blood glucose and histopathology of pancreas in alloxan-induced diabetic rats.

    Science.gov (United States)

    Edem, Do; Ekanem, Is; Ebong, Pe

    2009-07-01

    Effects of aqueous extract of alligator pear seed on normal and alloxan-induced diabetic rats were investigated in 6 groups of rats (5 rats per group). Test groups were made diabetic with intra-peritoneal injection of alloxan and treated with 300 mg and 600 mg/kg body weight of alligator pear seed extract. Two non-diabetic groups were also administered with 300 mg and 600 mg/kg body weight extract. The levels of blood glucose were examined in all 6 experimental groups. In diabetic rats, blood glucose levels were significantly reduced (pblood glucose levels were significantly reduced (palligator pear seed may contribute significantly to the reduction of blood glucose levels and can be useful in the treatment of diabetes.

  16. PENGARUH PEMBERIAN EKSTRAK BUAH PISANG (Musa acuminate L DAN BUAH ALPUKAT (Persea americana Mill TERHADAP BERAT HATI MENCIT (Mus musculus BETINA

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    Heny Utami Ningsih

    2018-02-01

    Full Text Available Male reproductive ability ability has a linear correlation with libido and testosterone. Both are associated with various things main food consumption such as protein, vitamins and minerals. Aphrodisiac  is an ingredient or drug that arouses sexual arousal or libido. Pumpkin is an example of food from plants with the highest content of zinc. This study was to determine whether there is a difference between the content of zinc on the peel, flesh and pumpkin seeds against sexual activity of mice. The experimental animals used were 24 male, group I mice as control group, Group II was grouped with pumpkin peel extract, Group III was grouped with extract of pumpkin flesh, Group IV was grouped with extract of pumpkin seeds. The dose used is 2gr / kg BB and volume of 0.2 ml is given for 35 days. Sexual Activity is observed every day  by the position of male mice riding females. Based on Anova One Way test results showed there are differences in sexual activity  mice males in each group (ρ 0.000. The difference of the result of the number of mounts indicated that the giving of yellow pumpkin extract had an effect on the increase of mating behavior of male mice. The group treated with the extract of pumpkin flesh showed the highest number of mice mounts (mean = 24,1667.Further research is needed to get a clearer picture of the composition of the pumpkin nutrients that indirectly affect fertility in men.

  17. Identification and characterization of a new Bacillus atrophaeus strain B5 as biocontrol agent of postharvest anthracnose disease in soursop (Annona muricata) and avocado (Persea americana).

    Science.gov (United States)

    Guardado-Valdivia, Lizeth; Tovar-Pérez, Erik; Chacón-López, Alejandra; López-García, Ulises; Gutiérrez-Martínez, Porfirio; Stoll, Alexandra; Aguilera, Selene

    2018-05-01

    Anthracnose is a fungal disease caused by Colletotrichum species that is detrimental to numerous fruit, including soursop and avocado. The use of fungicides to maintain the high quality of fruit creates a potential health risk. One alternative to this problem is the biological control, which has been applied successfully during postharvest. The Bacillus species are one of the most studied biological agents against postharvest pathogens because accomplish their biocontrol performance by producing a variety of metabolites. In this study, we evaluated the activity of metabolites contained in the cell free supernatant, obtained from Bacillus strain B5 culture, against micelial growth and spore germination of two virulent strains of C. gloeosporioides isolated from soursop and avocado. On the basis of 16S rDNA gene sequence analysis, this strain was identified as Bacillus atrophaeus. A preventive treatment using cell free supernatant, reduced severity and incidence of anthracnose disease on harvested soursop and avocado fruit. B. atrophaeus strain B5 harbors genes involved in the production of antibiotics such as surfactin, bacillomycin and iturin, which could be contributing to the efficiency of the preventive treatment during postharvest. The antagonistic role of metabolites contained in the cell free supernatant against anthracnose disease, provide a new approach by which to attack this problem and can help reduce the use of chemical pesticides, environmental pollution, leading to the safer fruit preservation. Copyright © 2018 Elsevier GmbH. All rights reserved.

  18. [High-density lipoproteins (HDL) size and composition are modified in the rat by a diet supplemented with "Hass" avocado (Persea americana Miller)].

    Science.gov (United States)

    Pérez Méndez, Oscar; García Hernández, Lizbeth

    2007-01-01

    To determine the effects of dietary avocado on HDL structure and their associated enzyme, paraoxonase 1 (PON1). Fifteen Wistar male rats received avocado as part of their daily meal (5 g by 17.5 g chow diet), keeping the caloric intake similar to the control group (n=15) that received their usual chow diet. After 5 weeks, HDL were isolated by sequential ultracentrifugation and their size and chemical composition were analyzed. PON1 was determined in serum spectrophotometrically using phenylacetate as substrate. Rats that received avocado had about 27% lower triglycerides plasma levels whereas their HDL-cholesterol was 17% higher as compared to control group. The mean HDL Stokes diameter was significantly lower in avocado group (11.71 +/- 0.8 vs. 12.27 +/- 0.26 nm, in control group, p avocado group. HDL structural modifications induced by avocado were not related to modifications of LCAT and PLTP activities, but occurred in parallel with higher serum levels of PON1 activity when compared to the controls (57.4 +/- 8.9 vs. 43.0 +/- 5.6 micromol/min/mL serum, p avocado in the diet decreased plasma triglycerides, increased HDL-cholesterol plasma levels and modified HDL structure. The latter effect may enhance the antiatherogenic properties of HDL since PON1 activity also increased as a consequence of avocado.

  19. Historia de la Tripanosomiasis Americana en Colombia

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    Fernando Serpa Florez

    2000-08-01

    Full Text Available

    La Academia Nacional de Medicina de Colombia rinde homenaje a Carlos Chagas, opónimo médico brasileño, nacido en Oliveira en 1879 y fallecido en Río de Janeiro en 1934, con motivo del nonagésimo aniversario del descubrimiento realizado por él del Trypanosoma cruzi, hazaña científica que bautizó en memoria de su maestro Osvaldo Cruz (1872 – 1917, fundador del Instituto de Manquinhos que hoy lleva el nombre de este sanitarista. (1

    Carlos Chagas fue miembro correspondiente de nuestra Corporación, elegido hace ochenta años, durante la presidencia del profesor Miguel Rueda Acosta, lo que hace que celebremos el triunfo de quien, honrosamente, podemos considerar como uno de los nuestros. (2 Chagas encontró en 1909 la relación existente entre la Tripanosomiasis Americana y el agente de esta dolencia descubierto por él, que por ello hoy se llama Enfermedad de Chagas.

    La tripanosomiasis americana o enfermedad de Chagas, es una entidad autóctona de nuestro continente que, como tantas otras, en un principio fue una zoonosis (enfermedad de los animales que se transmite al hombre tal cual la hidrofobia o rabia, la fiebre amarilla, la encefalitis equina, el carbón bacteridiano, la enfermedad de Lyme… para citar unas cuantas.

    La dolencia se extendió a la especie humana cuando ésta irrumpió en la hábitat del huésped intermedio del mal y se ha hecho cada vez más aparente con el aumento de la población y el desequilibrio ecológico que se presenta con la deforestación.

    Recordemos que sus reservorios (es decir los hospederos que le sirven de depósito, son algunos animales domésticos como el perro, el cerdo o el cobayo y peridomésticos y silvestres como las ratas, los murciélagos, los armadillos, monos y zarigüeyas. Al hombre, desde luego, podemos considerarlo como reservorio de esta dolencia. En Colombia los primeros informes científi-cos sobre animales domésticos y silvestres reservorios del Trypanosoma

  20. Evaluation of Treatments to Reduce Hardness of Agave americana Core

    Directory of Open Access Journals (Sweden)

    José A. Ramírez

    2006-01-01

    Full Text Available Agave americana contains inulin as storage carbohydrate. Therefore, agave is interesting to be used for the extraction of inulin by pressing. The yield of the process is low due to the high hardness of the core. The objective of this work was to evaluate pretreatments to reduce the hardness in the process of obtaining inulin by pressing. Treatments with water, sulphuric acid 1 % (by mass or sodium hydroxide 1 % (by mass were tested and optimized. The pretreatment of the core of A. americana with sulphuric acid 1 % allowed the reduction of hardness from 30 000 g to 2000 g of breaking force. The mathematical model obtained predicts an optimum processing at 84 °C during 75 min. The treatment with sulphuric acid 1 % also allows white core of A. americana to be obtained, while the other treatments provide yellow core. These results open a good alternative to obtain value added products from this resource.

  1. Composición esterólica de la fracción insaponificable del aceite de aguacate de distintas variedades

    Directory of Open Access Journals (Sweden)

    Martínez Nieto, L.

    1994-12-01

    Full Text Available The study of the sterolic composition of the unsaponifiable fraction of oil obtained from several varieties of avocados (Bacon, Fuerte, Hass, and Reed there is not any difference among several areas, sizes and pick up dates for the same variety, but there are differences that depend on the analysed variety, therefore it is possible to concluir that an itemized analysis of the sterolic composition of the oil may be an avocado variety indicator.

    El estudio de la composición esterólica de la fracción insaponificable de aceite obtenido de distintas variedades de aguacates (Bacon, Fuerte, Hass y Reed pone de manifiesto que no existen diferencias para una misma variedad incluso para distintas zonas, calibres y fechas de corte, pero si existen diferencias que dependen de la variedad analizada, pudiéndose concluir que un análisis detallado de la composición esterólica puede ser indicativo de la variedad de aguacate de que procede un aceite.

  2. APLICACIONES FOLIARES DE NITRATO DE CALCIO EN LA MADURACIÓN Y DAÑOS POR FRÍO EN AGUACATE 'FUERTE'

    Directory of Open Access Journals (Sweden)

    L. Saucedo-Hernández

    2005-01-01

    Full Text Available Con la finalidad de estudiar el efecto del calcio sobre la poscosecha de frutos de aguacate, árboles del cv. 'Fuerte' se asperjaron con Ca(NO32 al 0, 0.3 y 0.5 %. Se realizaron seis aplicaciones precosecha a partir del 4 de mayo del 2001, la cosecha se realizó en enero del 2002, una vez cosechados los frutos se almacenaron a temperatura ambiente y a 5 °C por cinco semanas, realizando evaluaciones a las 0, 3 y 5 semanas. Los resultados obtenidos indicaron que existió un incremento en el contenido de calcio en cáscara y pulpa en los frutos de aguacate tratados con nitrato de calcio. Las aplicaciones precosecha de nitrato de calcio al 0.3 y 0.5 % mejoraron la firmeza y redujeron la pérdida de peso de los frutos siendo diferentes al testigo, así también diminuyó la producción de CO2, etileno y daños por frío.

  3. Dinámica microbial del suelo asociada a diferentes estrategias de manejo de Phytophthora cinnamomi Rands en aguacate

    Directory of Open Access Journals (Sweden)

    Joaquín Guillermo Ramírez Gil

    2013-12-01

    Full Text Available La marchitez del aguacate es la enfermedad más limitante de este cultivo, cuyo agente causal más relevante es el oomycete Phytophthora cinnamomi Rands. Es por esto que se han desarrollado diferentes estrategias para su manejo integrado, pero aún prevalece el uso de productos químicos, como única medida de manejo, generando impactos negativos en el ambiente y la salud. Uno de los efectos perjudiciales que se ocasiona es la alteración de las poblaciones microbianas en el suelo. Este trabajo estuvo encaminado a conocer la dinámica microbiana del suelo, bajo diferentes estrategias de manejo de esta enfermedad, para lo cual se midió su dinamismo mediante unidades formadoras de colonias (UFC, para hongos, bacterias y actinomicetos, a partir de muestras de suelo y rizósfera de la raíz, bajo incubación en condiciones de anaerobiosis y aerobiosis, además se midió la actividad microbiana total, en condiciones de laboratorio, como complemento se cuantificaron microorganismos como: Trichiderma spp, bacterias formadoras de endosporas (BAFE, celulolíticos, proteolíticos, amilolíticos, solubilizadores de fosfato, fijadores asimbióticos de nitrógeno y promotores del crecimiento, como Pseudomonas spp., fluorescentes. Los resultados encontrados en esta investigación, sugieren que el uso individual y combinado de mantillo orgánico, material compostado de estiércol bovino, enmienda mineral y cascarilla de arroz y la propuesta de integración; incrementan significativamente la población y actividad microbiana aerobia, en la cual se identificaron microorganismos antagonistas como, Trichiderma spp., celulolíticos, Pseudomonas spp. fluorescentes y BAFE.

  4. Fenología del aguacate 'hass' en el clima semicálido de Nayarit, México

    Directory of Open Access Journals (Sweden)

    L. E. Cossio-Vargas

    2008-01-01

    Full Text Available Este estudio se desarrolló en varios periodos del 2000 al 2005, en dos huertos comerciales de aguacate 'Hass' en los municipios de Tepic y Xalisco, Nayarit, México. El objetivo fue determinar los ciclos de crecimiento de árboles de 'Hass' cultivados sin riego. El cv. Hass presentó dos flujos de crecimiento vegetativo: invierno (mayor intensidad y verano (menor intensidad. El proceso completo de desarrollo floral, de yema cerrada a antesis, en brotes del flujo vegetativo de invierno y verano fue de 11.5 meses y 7.5 meses, respectivamente. La máxima producción de raíces ocurrió en agosto. La máxima intensidad (45 % de la "caída de fruto de junio" ocurrió durante las primeras lluvias de verano (junio. El crecimiento en longitud del fruto, desde el amarre (marzo hasta cosecha (noviembre, duró ocho meses. Los promedios anuales de temperaturas máximas del aire fluctuaron de 26.8 a 33.4 °C y las mínimas de 9.3 a 20.4 °C. El promedio de las temperaturas máximas mensuales del suelo se mantuvo todo el año entre 20.1 y 24.5 °C, y las mínimas entre 18.5 y 23.4 °C. El nivel de humedad del suelo fue >85 % desde la estación lluviosa (junio a octubre hasta diciembre; posteriormente descendió hasta alcanzar 65 % en mayo.

  5. EVALUACIÓN QUIMICA DE LA FIBRA EN SEMILLA, PULPA Y CÁSCARA DE TRES VARIEDADES DE AGUACATE

    Directory of Open Access Journals (Sweden)

    ADELA MARÍA CEBALLOS P

    Full Text Available El sector agropecuario colombiano requiere integrarse al sector agroindustrial para aumentarle valor a sus productos y residuos. Utilizar residuos agrícolas y agroindustriales como materias primas para obtener metabolitos secundarios es tema de interés en investigación y busca dar respuestas al uso de esos residuos, mediante desarrollo de tecnologías aplicables al entorno colombiano. Se valoraron cáscara, semilla y pulpa de aguacate variedades Booth8, Trinidad y Papelillo en estados de madurez fisiológica (MF y consumo (MC. Se hizo caracterización bromatológica, contenido de fibra como carbohidratos solubles (CHOS, lignina LG, celulosa y hemicelulosa (CEL y HEMICEL y fibra bruta (FB según (Tappi Methods 1993, 1998, AOAC, 2001. Los resultados fueron analizados mediante ANOVA (P < 0,05 utilizando SAS V9.1. Los CHOS en los dos estados de madurez y para las tres variedades variaron entre 12-38% cáscara, 20-35% pulpa y 17-35% semilla; CEL y HEMICEL 8-50% cáscara, 7-26% pulpa, 1-5% semilla, los cuales pueden ser útiles como complementos de procesos biotecnológicos. La pulpa de las tres variedades tiene alto valor nutricional por sus contenidos de proteína, fibra y minerales. La composición de la cáscara y semilla podrían ser importantes para suplementos de dieta animal o como recuperador de suelos para la producción agrícola

  6. faloabi@uniben.edu Antiproliferative and Pro-apoptotic activities

    African Journals Online (AJOL)

    MICHAEL HORSFALL

    Keyword: Persea americana, antiproliferative activity, apoptotic effect, flow ... of the stem bark of Persea americana in MCF-7 cell line by flow cytometer. .... of an electric milling machine. ... Flow Cytometric Measurement Of Cell Proliferation:.

  7. Organização estrutural da casca de Persea major Kopp (Lauraceae Structural organization of Persea major Kopp bark (Lauraceae

    Directory of Open Access Journals (Sweden)

    Leila Teresinha Maranho

    2009-06-01

    Full Text Available O presente estudo foi desenvolvido com o objetivo de contribuir ao conhecimento da estrutura da casca de Persea major Kopp (Lauraceae, espécie nativa da América do Sul. No Brasil, é conhecida, popularmente, como "pau de andrade" e utilizada na cultura tradicional na cicatrização dos ferimentos. A análise da casca foi feita usando microscopia de óptica. A casca se distingue, principalmente, pelo tipo e distribuição dos tecidos esclerenquimáticos e a presença de células oleíferas e mucilaginosas. As características encontradas na estrutura da casca desta espécie mostram grande importância para a identificação e servem como parâmetros no controle de qualidade. Estas células oleíferas e mucilaginosas com uma distribuição restrita nas dicotiledôneas constituem uma característica taxonômica e diagnóstica particularmente valiosa.The study was undertaken to increase our knowledge of bark structure of Persea major Kopp (Lauraceae, a native specie from South America. In Brazil, it is known as "pau de andrade" and is used by traditional cultures to heal injuries. This analysis was done by light microscopy. The bark is distinguished mainly by the type and distribution of sclerenchymatic tissues, and the presence of oil and mucilage cells. The characteristics found in the bark anatomy of this species are of great importance for identification purposes and as parameters in quality control. These oil and mucilage cells have a restricted distribution in the dicotyledons and constitute a particularly valuable taxonomic and diagnostic feature.

  8. Tratamento da leishmaniose tegumentar americana pelo niridazol

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    Miroslau Constante Baranski

    1970-08-01

    Full Text Available Foi empregue o niridazol um derivado do nitrotiazol, em doze pacientes com leishmaniose tegumentar americana. Em todos êles, o diagnóstico clínico da doença foi confirmado pela biópsia das lesões e em oito dêles também pela positividade da intradetermorreação de Montenegro. Oito pacientes tinham lesões mucosas metastáticas em atividade e lesões cutâneas iniciais cicatrizadas há tempo mais ou menos longo. Dois apresentavam concomitância de lesões cutâneas e mucosas em atividade, e dois tinham lesões cutâneas exclusivas. A duração da doença variou de 2 meses a 32 anos. A posologia diária do niridazol foi uniformememente de 25 mg/kg de pêso corpóreo. O medicamento foi administrado por via oral, em duas tomadas diárias, sempre com o doente internado em hospital. Quando a tolerância o permitia, o paciente recebia cinco séries de tratamento de 10 dias de duração cada, intercaladas por períodos áe suspensão da droga de 10 dias entre uma série e outra. Isso foi possível em 10 dos 12 pacientes. O tratamento foi bem tolerado em 5 doentes e de tolerância regular em 4. Mal tolerado em um paciente pela ocorrência de alucinações e excitação mental e interrompido pela péssima tolerância em dois doentes devido ao aparecimento de convulsões generalizadas com perda da consciência. O tratamento acompanhou-se de grande incidência, de efeitos colaterais. A ocorrência de para-efeitos mais intensos não foi devida às más condições hepáticas. Provas de função hepática. hemogramas, estudos bioquímicos do sangue e exames de urina, realizados antes, durante e após o tratamento, não revelaram alterações significativas. Biópsias hepáticas por punção com agulha em dez doentes, prévias e posteriores ao tratamento, não detectaram lesões hepáticas que pudessem ser atribuídas à medicação. O seguimento dos doentes prolongou-se pelo prazo de dois a 36 meses apos o tratamento. Alterações eletrocardiogr

  9. Agrobacterium-mediated genetic transformation of Fraxinus americana hypocotyls

    Science.gov (United States)

    Kaitlin J. Palla; Paula M. Pijut

    2015-01-01

    An Agrobacterium tumefaciens-mediated genetic transformation system was successfully developed for white ash (Fraxinus americana) using hypocotyls as the initial explants. Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 µM 6-benzyladenine (BA) and 0.5 µM...

  10. Memory Consolidation and Gene Expression in "Periplaneta Americana"

    Science.gov (United States)

    Strausfeld, Nicholas J.; Pinter, Marianna; Lent, David D.

    2005-01-01

    A unique behavioral paradigm has been developed for "Periplaneta americana" that assesses the timing and success of memory consolidation leading to long-term memory of visual-olfactory associations. The brains of trained and control animals, removed at the critical consolidation period, were screened by two-directional suppression subtractive…

  11. Drug interaction studies of Ximenia americana and Pavetta ...

    African Journals Online (AJOL)

    The therapeutic efficacy of single or multicomponent herbs is thought to reside in synergistic interactions between the bioactive constituents. The methanol extracts of X. americana and P. crassipes were initially screened against Gram positive and negative organisms as well as against Mycobacterium tuberculosis H37Rv ...

  12. Enfermedad de Chagas o Tripanosomiasis Americana.

    Directory of Open Access Journals (Sweden)

    Felipe Guhl

    2000-08-01

    Full Text Available

    Situación Actual de Colombia.

    La enfermedad de Chagas o tripanosomiasis americana es una enfermedad parasitaria crónica causada por un protozoario flagelado el Trypanosoma cruzi, descrito por primera vez por Carlos Chagas, médico brasileño, a comienzos de este siglo y en su honor se denominó la enfermedad que lleva su nombre.

    Este parásito normalmente se transmite al ser humano a través de insectos triatomíneos estrictamente hematófagos de la familia Reduviidae, en el momento en que perforan la piel para succionar la sangre que los alimenta.

    Sin embargo, no se inocula directamente por intermedio de las estructuras bucales del insecto en el momento de la picadura como en el caso de las tripanosomiasis africanas, si no que se deposita pasivamente en la piel a través de las heces del insecto, penetrando en el cuerpo por la herida que causa la picadura u otras abrasiones de la piel o de la mucosa. El T. cruzi, también puede transmitirse por infección congénita, por transfusión de sangre contaminada o por el transplante de órganos contaminados. El ciclo vital del parásito es largo y complejo y su desarrollo tiene varias etapas, tanto en el vector triatomineo como en el huésped vertebrado .

    La Enfermedad de Chagas constituye una amenaza permanente para casi la cuarta parte de toda la población de América Latina. Si bien la enfermedad se encuentra presente en toda América Central y del Sur, sus manifestaciones y características epidemiológicas son altamente variables entre una y otra zona endémica. Existe una gran diversidad en las tasas de prevalencia, formas de transmisión, características parasitarias, patología clínica, vectores y reservorios.

    Más que cualquier otra enfermedad parasitaria, la enfermedad de Chagas se relaciona con el desarrollo económico y social de la población: los insectos triatomineos y las enfermedades que ellos transmiten existirán mientras en

  13. Prototipo autotérmico móvil para producción de biocarbón con biomasa de esquilmos de aguacate

    Directory of Open Access Journals (Sweden)

    Lenin Ejecatl Medina Orozco

    2018-04-01

    Full Text Available El estado de Michoacán, México, es el principal productor mundial de aguacate, con un impacto social y económico en función de la superficie cultivada. Productos secundarios de esta actividad, son los esquilmos agrícolas derivados de las podas de mantenimiento de los árboles. El biocarbón (biochar de subproductos de aguacate, se vislumbra como una alternativa económica, ambiental y socialmente viable, para producir biocarbón en grandes volúmenes, sin extracción de maderas de bosque y selvas prístinas. El objetivo del presente estudio, fue construir y evaluar el desempeño en condiciones de campo, de un prototipo de biocarbón-pirólisis autotérmico y móvil, con volumen útil de 1.7 m3 de biomasa triturada. El equipo fue construido de acero inoxidable, para darle un periodo de vida útil de 7 años bajo condiciones intensivas de uso, con movilidad dentro de los campos agrícolas y auto térmico para no depender de una fuente de calor externa. El equipo fue de bajo costo comparado con equipos comerciales ($ 700 000.00 pesos MN y con un potencial futuro en la cogeneración de energía. La principal variable en el costo de fabricación es el precio del acero inoxidable en el mercado. El equipo reportó una tasa de conversión de biomasa a biocarbón, en condiciones de campo, de entre 300 y 400 kg por día, con una tasa promedio de recuperación del 16% en biocarbón. Las principales variables en la transformación de la biomasa a biocarbón fueron: humedad, el tamaño de la astilla y condiciones atmosféricas. Se encontró una Capacidad de Intercambio Catiónico (CIC de 46 Cmolc kg-1 y pH 10.25. El equipo transformó satisfactoriamente la biomasa de aguacate y el biocarbón presentó características adecuadas para su uso en suelos volcánicos.

  14. Determinación de tamaño de muestra de características del xilema de brote de aguacate mediante remuestreo

    Directory of Open Access Journals (Sweden)

    Elizabeth Meza-Castillo

    2010-12-01

    Full Text Available El propósito de este trabajo fue determinar el tamaño de muestra adecuado, para detectar diferencias estadísticas en análisis de varianza de los caracteres de xilema, en brotes anuales de aguacate. Para esto, fueron registradas en tres árboles con altura contrastante las variables frecuencia, área, perímetro y diámetro de los vasos del xilema. A partir de 50 observaciones de cada árbol, se realizaron 5.000 remuestreos no paramétricos, para cada repetición de 2 a 50 campos microscópicos. A partir del tamaño de muestra de 15 repeticiones, las desviaciones en los cinco estadísticos son mínimas, por lo que se propone como el mínimo para el muestreo de dichas variables.

  15. Los pequeños productores de aguacate del Ejido y la Comunidad de San Francisco Peribán, Michoacán (México

    Directory of Open Access Journals (Sweden)

    Cristina Steffen Riedemann

    2003-01-01

    Full Text Available Una reiterada recomendación que las instituciones públicas vinculadas al agro hacen a los campesinos, es que supriman de su patrón de cultivos los productos en que México no tiene ventajas comparativas como los granos y se reconviertan hacia aquellos que puedan colocar en el mercado obteniendo mayor rentabilidad: frutas y hortalizas. En el presente trabajo se analiza el caso de ejidatarios y comuneros, quienes desde hace algunas décadas han emprendido la producción de aguacate, producto netamente comercial y potencialmente exportable. A lo largo del análisis se señala, por una parte, cuáles son los beneficios que esta actividad ha aportado a los productores y, por otra, las limitaciones a que se enfrentan al competir en un mercado abierto, controlado por grandes productores y/o por comercializadoras transnacionales.

  16. CARACTERÍSTICAS MORFOLÓGICAS Y BIOQUÍMICAS DE FRUTOS DE CHINENE (Persea schiedeana Nees.

    Directory of Open Access Journals (Sweden)

    J. G. Cruz-Castillo

    2007-01-01

    Full Text Available El chinene es un árbol frutal nativo de Mesoamérica y forma parte de la familia Lauraceae. Su consumo, como buena fuente nutricional es reconocido en regiones tropicales de México y Centroamérica. Sin embargo, poco se conoce sobre su contenido nutrimental. Para ello, se llevaron a cabo análisis de ácidos grasos en pulpa de chinene por dos años consecutivos utilizando cromatografía de gases. Se obtuvieron concentraciones de ácido oleico, y palmitico parecidas a las encontradas en aguacate. Las concentraciones de ácido oleico fueron comparables con las del aguacate 'Hass'. En otro estudio, se midieron morfológicamente frutos de chinene provenientes de varias comunidades de la región central de Veracruz, México. Resultó una gran variación en tamaño, peso, y contenido de pulpa. Asimismo, el patrón de crecimiento de los frutos fue caracterizado por una curva simple sigmoide con un acumulado de 1635.11 unidades calor arriba de 6 oC, desde el amarre de fruto hasta la cosecha.

  17. Antihyperglycemic effect of Persea duthieion blood glucose levels and body weight in alloxan induced diabetic rabbits.

    Science.gov (United States)

    Sultan, Khushbakht; Zakir, Muhammad; Khan, Haroon; Khan, Ihsaan Ullah; Ayaz, Sultan; Khan, Iqbal; Khan, Jafar; Khan, Murad Ali

    2016-05-01

    The present study was designed to investigate the antihyperglycemic effect of Persea duthieion blood glucose concentration and body weight in alloxan induced diabetic hyperglycemic rabbits. The results illustrated significant antihyperglycemic activity of crude extract with 17.44% and 28.02% amelioration at 25 and 50mg/kg p.o. respectively after 24th day of drug treatment; equally supported by body weight recovery. Upon fractionation, most dominant antihyperglycemic effect was displayed by aqueous fraction with 22.12% and 34.43% effect followed by ethyl acetate fraction with 24.32% and 32.05% effect at 25 and 50mg/kg p.o. respectively after 24th day of drug treatment. The effect on blood glucose was also reflected on body weight of animals. In conclusion, our study documented marked antihyperglycemic activity of extract/fractions of P. duthiei.

  18. Essential oils of Persea povedae W.C. Burguer (Lauraceae) of Costa Rica

    International Nuclear Information System (INIS)

    Hernandez, Lorena; Ciccio, Jose F.; Chaverri, Carlos

    2006-01-01

    The tree Persea povedae W. C. Burguer, grows in wild form in Costa Rica. The analyses to determine the composition of the essential oils that are extracted from the branches and leaves of the tree were effected by means of gas chromatography technique in capillary column, using Flame Ionization Detector (FID) and mass spectrometry (GC/MS). Hundred and ten (110) compounds were identified. The major constituents of the branches are: α-pinene (7,7%), linalool (7,6%), espathulenol (5,1%), α-copaene (4,8), dodecanal (4,4%), Ε-9-dodecenal (4,1%), β -pinene (3,2%), γ-muurolene (3,1%) and α-cadinene (3,0%). The main constituents of the leaves are: Ε-nerolidol (14,6%), pentadecanoic acid (ca. 14%), longipinanol (5,1%), linalool (4,5%), γ-muurolene (3,8%) y espathulenol (3,8%). (author) [es

  19. Molecular phylogenetic analysis of the Persea group (Lauraceae) and its biogeographic implications on the evolution of tropical and subtropical Amphi-Pacific disjunctions.

    Science.gov (United States)

    Li, Lang; Li, Jie; Rohwer, Jens G; van der Werff, Henk; Wang, Zhi-Hua; Li, Hsi-Wen

    2011-09-01

    The Persea group (Lauraceae) has a tropical and subtropical amphi-pacific disjunct distribution with most of its members, and it includes two Macaronesian species. The relationships within the group are still controversial, and its intercontinental disjunction has not been investigated with extensive sampling and precise time dating. • ITS and LEAFY intron II sequences of 78 Persea group species and nine other Lauraceae species were analyzed with maximum parsimony and Bayesian inference. Divergence time estimation employed Bayesian Markov chain Monte Carlo method under a relaxed clock. • Several traditional genera or subgenera within the Persea group form well-supported monophyletic groups except Alseodaphne and Dehaasia. The divergence time of the Persea group is estimated as ∼55.3 (95% higher posterior densities [HPD] 41.4-69.9) million years ago (mya). Two major divergences within the Persea group are estimated as ∼51.9 (95% HPD 38.9-63.9) mya and ∼48.5 (95% HPD 35.9-59.9) mya. • Persea can be retained as a genus by the inclusion of Apollonias barbujana and exclusion a few species that do not fit into the established subgenera. A major revision is recommended for the delimitation between Alseodaphne, Dehaasia, and Nothaphoebe. We suggest that the Persea group originated from the Perseeae-Laureae radiation in early Eocene Laurasia. Its amphi-pacific disjunction results from the disruption of boreotropical flora by climatic cooling during the mid- to late Eocene. The American-Macaronesian disjunction may be explained by the long-distance dispersal.

  20. American wild celery (Vallisneria americana): Ecological considerations for restoration

    Science.gov (United States)

    Korschgen, C.E.; Green, W.L.

    1988-01-01

    The success of vegetation management programs for waterfowl is dependent on knowing the physical and physiological requirements of target species. Lakes and riverine impoundments that contain an abundance of the American wildcelery (Vallisneria americana ) have traditionally been favored by canvasbacks (Aythya valisineria ) and other waterfowl as feeding areas during migration. Information on the ecology of American wildcelery is summarized to serve as a guide for potential wetland restoration projects. Techniques are described for transplanting winter buds. Management programs that employ these techniques should define objectives clearly and evaluate the water regime carefully before initiating major restoration.

  1. Distribution and characterization of Heterobilharzia americana in dogs in Texas.

    Science.gov (United States)

    Rodriguez, J Y; Lewis, B C; Snowden, K F

    2014-06-16

    Heterobilharzia americana is a trematode parasite (family Schistosomatidae) that infects a wide range of wild mammalian hosts. Canine cases have been reported in the Gulf coast and south Atlantic states, Kansas, and Oklahoma. A total of 238 canine H. americana cases in Texas were retrospectively collected for a period of approximately 22 years from case records at the Texas Veterinary Medical Diagnostic Laboratory and the Veterinary Medical Teaching Hospital pathology service, diagnostic parasitology service, and Gastrointestinal Laboratory at Texas A&M University College of Veterinary Medicine. Of these cases, 26 patients had 1-2 repeat positive tests for a total of 268 positive tests (26 biopsies, 39 necropsies, 160 fecal examinations, and 43 PCR). Multiple dogs were infected in 12 households. Cases were distributed primarily in the eastern region of Texas in 42 of 254 counties. Cases were seen as far west as Kerr county and in counties bordering Oklahoma, Louisiana, Mexico, and the Gulf of Mexico. The median dog age was 5.6 years (2.7 months to 17.2 years) and the median weight was 20.5 kg (1-61.6 kg). All American Kennel Club (AKC) breed groups were represented (n=186): crossbred (20%), herding (17.8%), sporting (16.1%), toy (10.8%), hounds (10.8%), working (10.1%), terrier (8.5%), non-sporting (4.9%), and miscellaneous (1%). No seasonal pattern of diagnosis was apparent. Clinical signs reported (n=90) were diarrhea (67%), weight loss (38%), anorexia/hyporexia (27%), vomiting (22%), hematochezia (20%), lethargy (17%), polyuria/polydipsia (6%), and collapse (3%). In 39 necropsy cases, trematode eggs were identified by histopathology in the small intestine (84%), liver (84%), large intestine (39%), pancreas (35%), lung (9%), lymph node (8%), spleen (4%), and stomach (3%). Adult parasites were identified histologically in four cases. Granulomatous inflammation associated with the eggs was the most commonly reported histopathologic change. Other changes reported

  2. Establishing of Agave Americana industry in South Africa

    CSIR Research Space (South Africa)

    Boguslavsky, A

    2007-09-01

    Full Text Available of the sisal decorticator was therefore modified to make it more suitable for the processing of Agave Americana leaves. Steel side plates were attached to the rotor to prevent the blockage of the machine. The feeding tray and beater bars were modified... to accommodate the side plates of the enlarged rotor. For added operator safety, a “tunnel” guard was also added the feeding tray through which the leaves are fed. The size of this was such that the Agave leaf could fit into it but without any space...

  3. American Narcissus: Lacanian Reflections on DeLillo’s Americana

    Directory of Open Access Journals (Sweden)

    Graley Herren

    2016-05-01

    Full Text Available Don DeLillo’s first novel, 'Americana' (1971, is assembled from a toolkit of psychological tropes. Critics have long recognized the novel’s debts to (and parodies of Sigmund Freud, leading one critic to dub protagonist David Bell as “the American Oedipus.” The present article reconsiders the novel from the perspective of Jacques Lacan’s psychoanalytic theories. Several of Lacan’s key concepts—including his seminal articulation of the Mirror Stage, his revisions to Freud’s Oedipus Complex, his emphasis on lack as the engine of desire, as well as his reflections on dreams, fantasies, and the gaze—help shed new light on DeLillo’s splintered portrait of postmodern subjectivity in 'Americana'. The article uses Lacan to explain how and why each of David Bell’s attempts at indulging his incestuous maternal desires ends in disappointment, with particular emphasis on his three failed attempts at restaging the primal scene. Lacanian psychology provides a conceptual framework and useful taxonomy for understanding the shifting desires, misrecognitions, obfuscations, deflections, projections, and self-reflections of the American Narcissus, David Bell.

  4. Evolución del contenido de ácidos grasos de aceite de aguacate durante la maduración

    Directory of Open Access Journals (Sweden)

    Martín Nieto, L.

    1995-04-01

    Full Text Available The minimum fat richness of avocados necessary for marketing of the fruit is 8%, although after vegetative maturation values of about 20% or more are reached, the values depending on the variety. In the period between harvesting, when commercial maturity is reached, and full maturation, not only is the fat content increased but changes also occur in the fat composition, the unsaturated fatty acids increasing while the amount of saturated fatty acids decreases.

    La riqueza grasa mínima exigida al aguacate para su salida al mercado es del 8%, aunque en la maduración vegetativa se alcanzan valores de alrededor del 20% o más según variedades. En el período que transcurre desde el corte del fruto, de acuerdo con la madurez comercial, hasta la completa madurez del mismo no sólo aumenta la riqueza grasa, sino que se ha observado una evolución del contenido graso, de forma que hay un aumento de los ácidos grasos Insaturados en detrimento del contenido en ácidos grasos saturados.

  5. Aspersiones de calcio en la concentración nutrimental de hoja, cáscara y fruto de aguacate 'hass'

    Directory of Open Access Journals (Sweden)

    J. Herrera-Basurto

    2007-01-01

    Full Text Available Con la finalidad de estudiar el efecto del Ca(NO32, se realizaron aspersiones foliares precosecha en árboles de aguacate cv. Hass con concentraciones de 0, 0.3 y 0.5 %, cada quince días a partir de la segunda quincena de septiembre del 2003 hasta principios de enero del 2004, con un total de 11 aplicaciones. Se evaluó la concentración nutrimental en hoja, cáscara y pulpa. Los resultados indicaron que no hubo incremento de la concentración para N, P, K, Ca y Mg en hoja, cáscara y pulpa durante el primer año de evaluación; concluyéndose que en el segundo año se presentó un incremento de Ca en cáscara y pulpa, más no en hoja, dejando evidencia de un efecto residual.

  6. Nutrición de boro en aguacate cv. Hass: aspectos fisiológicos, morfológicos y ecológicos en un andosol de Michoacán

    OpenAIRE

    Castillo Vega, Andrea

    2012-01-01

    El cultivo de aguacate cv. Hass es una actividad en constante expansión debido a la demanda de exportación del fruto. La nutrición con B es uno de los factores de producción determinantes para la obtención de frutos tanto en la cantidad como de la calidad requerida para cumplir con los estándares internacionales. La obtención de este fruto en huertos comerciales de Michoacán, principal región productora del mundo, no ha sido caracterizada en cuanto a nutrición con B englob...

  7. Heterobilharzia americana infection and congestive heart failure in a llama (Lama glama).

    Science.gov (United States)

    Corapi, W V; Eden, K B; Edwards, J F; Snowden, K F

    2015-05-01

    The schistosome Heterobilharzia americana infects several mammalian species in the southeastern United States, including horses, but infections have not been reported in camelids. This is a report of H. americana infection in a 6-year-old llama with extensive cardiac pathology and congestive heart failure. Parasite-induced granulomas were widely disseminated and included overwhelming involvement of the lungs and liver. Microscopic lesions in the heart included myofiber degeneration and necrosis, with extensive replacement fibrosis. Polymerase chain reaction amplification and sequencing confirmed the presence of H. americana in the lungs. © The Author(s) 2014.

  8. Componentes volátiles de mamey (mammea americana L.

    Directory of Open Access Journals (Sweden)

    Alicia Lucía Morales

    2010-07-01

    Full Text Available Los componentes volátiles del aroma de mamey (Mammea americana L, fueron extraídos utilizando el método de destilación por arrastre con vapor-extracción simultánea con solvente orgánico. El extracto fue prefraccionado por cromatografía en columna en silica gel con gradiente discontinuo Pentano: Éter etílico para obtener tres fracciones que fueron analizadas por CGAR y CGAR-EM. Se detectaron 34 compuestos, de los cuales fueron identificados 22, siendo los componentes mayoritarios: Furfural (7281 ^ig/kg y E-Famesol (2145 ng/kg

  9. Distribution of 137Cs in the American Coot (Fulica americana)

    International Nuclear Information System (INIS)

    Potter, C.M.; Brisbin, I.L. Jr.; McDowell, S.G.; Whicker, F.W.

    1989-01-01

    Caesium-137 concentrations were determined for major tissue and organ components of Americal Coots (Fulica americana) wintering on two contaminated reactor cooling reservoirs on the US Department of Energy's Savannah River Plant. Concentrations reflected the relative contamination levels of the two reservoirs. A low ratio of 137 Cs in gut contents to that in muscle indicated that the coots used in the study were established winter residents and tissue 137 Cs had probably reached equilibrium with intake. The contributions of each component to the whole body weight and total 137 Cs body burden were calculated. Skeletal muscle exhibited the highest 137 Cs concentrations and comprised 35% of the total 137 Cs body burden. Gut contents represented 17% of the total body burden. Various other organs were intermediate and bone exhibited the lowest 137 Cs concentrations. Linear regressions were determined for 137 Cs concentrations in skeletal muscle, liver, kidney, gastrointestinal (GI) tract tissue and gut contents, as functions of whole-body concentrations. (author)

  10. Volumetry of Genipa americana in homogeneous plantation in Southwest Bahia

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    Mislene Barbosa Rocha

    2015-12-01

    Full Text Available This research aim to evaluate the volumetric estimates obtaining for Genipa americana, commonly known as jenipapeiro, in pure plantation in the municipality of Vitória da Conquista, Bahia State, Brazil. To determine individual volume, 100 standing trees were rigorously cubed. Ten volumetric models were adjusted. The best models were selected based on the selection criteria of weighted value of statistical parameters scores and residues distribution. Volume estimates were obtained by form factor and by adjusted equations. To validate the estimates, the calculate volumes were compared to measured data. Among the used methods to predict wood volume, the adjusted volumetric equations are recommended. Spurr (Log model present the best performance to estimate total wood volume.

  11. Morfoanatomia de folha e caule de Genipa americana L., Rubiaceae

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    Marianna Erbano

    2010-10-01

    Full Text Available Genipa americana L., conhecida como jenipapo, é uma Rubiaceae nativa do Brasil e as suas folhas são utilizadas pela população como antidiarreico e febrífugo, e o caule como anti-hemorrágico, contra luxações e contusões. Com o objetivo de caracterizar macro e microscopicamente folhas adultas e caules jovens para o controle de qualidade farmacognóstico, essa planta medicinal foi analisada segundo a metodologia clássica de morfoanatomia vegetal. Genipa americana apresenta folhas simples, opostas, de formato obovado a oblanceolado e pecíolos curtos. A epiderme foliar é uniestratificada e revestida por cutícula delgada e levemente estriada. Os estômatos são paracíticos e restritos à superfície abaxial. Há tricomas tectores uni e pluricelulares predominantemente na superfície abaxial. O mesofilo é dorsiventral. Em secção transversal, a nervura central é plano-convexa e o pecíolo é circular, ambos apresentando um feixe vascular colateral em disposição cilíndrica e outros menores. O caule possui felogênio localizado nas camadas subepidérmicas, colênquima anelar e um cilindro floemático externo ao de xilema, sendo ambos percorridos por raios parenquimáticos estreitos. Ocorrem idioblastos contendo compostos fenólicos, areia cristalina e drusas de oxalato de cálcio na folha e no caule.

  12. Swimming black-crowned night-herons (Nycticorax nycticorax) Kleptoparasitize American coots (Fulica americana)

    DEFF Research Database (Denmark)

    Graves, Gary R.

    2015-01-01

    I observed black-crowned night-herons (Nycticorax nycticorax) swimming and kleptoparasitizing American coots (Fulica americana) at an artificial lake in Pinal County, Arizona. This appears to be the first record of interspecific kleptoparasitism by a swimming ardeid.......I observed black-crowned night-herons (Nycticorax nycticorax) swimming and kleptoparasitizing American coots (Fulica americana) at an artificial lake in Pinal County, Arizona. This appears to be the first record of interspecific kleptoparasitism by a swimming ardeid....

  13. A entrada de franquias americanas no Brasil: uma estratégia diferente

    OpenAIRE

    Gurtner, Werner Frank

    2010-01-01

    Escrita para o público americano, destaca peculiaridades administrativas e culturais do mercado brasileiro de fast food. Usa exemplos dos procedimentos de entrada de algumas franquias americanas e seus consequentes sucessos e fracassos. Concentra-se, particularnente, no desempenho administrativo destas franquias no Brasil. Discute estratégias alternativas para a expansão internacional futura das franquias americanas. Written for an American audience, highli...

  14. A Case of Ventilator-Associated Pneumonia Caused by Ewingella americana : First Report from Turkey

    Directory of Open Access Journals (Sweden)

    Kenan Ecemiş

    2014-09-01

    Full Text Available Ewingella americana is a very rare cause of serious infections, especially in immunocompromised patients. We report a case of ventilator-associated pneumonia (VAP in an intensive care unit patient, who was treated successfully with ceftriaxone. To the best of our knowledge, this is the first case of VAP due to Ewingella americana in the English literature.J Microbiol Infect Dis 2014; 4(3: 118-120

  15. VALIDACIÓN DE MODELOS DE PREDICCIÓN DEL DESARROLLO FLORAL DEL AGUACATE 'HASS' DESARROLLADOS PARA NAYARIT, EN VARIOS CLIMAS DE MICHOACÁN

    Directory of Open Access Journals (Sweden)

    S. Salazar-García

    2009-01-01

    Full Text Available Utilizando registros de temperatura ambiental y desarrollo floral obtenidos de 1998 al 2006 de una zona con clima semicálido subhúmedo del estado de Nayarit, se desarrollaron cinco modelos de predicción del desarrollo floral para brotes de los flujos vegetativos de invierno y verano de aguacate "Hass". El objetivo de esta investigación fue evaluar los modelos de predicción generados en Nayarit, para estimar capacidad para predecir el desarrollo floral de brotes de los flujos vegetativos de invierno, primavera y verano de "Hass" en los principales climas de la región aguacatera del estado de Michoacán. El modelo de predicción veranoDFA¿19 desarrollado en Nayarit, mostró una elevada capacidad de predicción del desarrollo floral en brotes del flujo de verano (R2 = 0.94, para un conjunto de datos de los cuatro climas del estado de Michoacán en donde se concentra el 85 % de la superficie aguacatera: cálido subhúmedo Aw1(w, semicálido subhúmedo (AC(w0(w, semicálido subhúmedo (AC(w1(w + (AC(w2(w y templado subhúmedo C(w2(w. Los demás modelos de predicción probados en Michoacán no pronosticaron el desarrollo floral en brotes de los flujos vegetativos de invierno, primavera o verano en ninguno de los climas estudiados.

  16. As bibliotecas nacionais latino americanas e o capital social

    Directory of Open Access Journals (Sweden)

    Deborah Medeiros

    2017-01-01

    Full Text Available Resumo Estudo que pretende analisar o uso da web social nos serviços das bibliotecas nacionais latino americanas e as formas de fomentar Capital Social em sua comunidade. Buscou-se verificar quais mídias sociais as bibliotecas nacionais usam, avaliando como adaptam aos serviços das bibliotecas e como as utilizam para fomentar o Capital Social, adotando-se para isso a teoria do fator whuffie. Utilizou-se a coleta e mapeamento de mídias sociais e observação não participante para a análise dos websites das Bibliotecas Nacionais analisadas. Como resultado, a pesquisa constatou que as Bibliotecas Nacionais em sua grande maioria utilizam seus sites e bibliotecas digitais para oferecer serviços e fomentar Capital Social entre seus interagentes. Por fim, conclui-se que as bibliotecas analisadas ainda utilizam suas mídias sociais na internet para fins administrativos e de divulgação, e observa-se que, através de algumas práticas identificadas nas bibliotecas analisadas nesta pesquisa, as demais bibliotecas podem buscar novas formas e possibilidades de interação dos seus serviços com seus interagentes por meio dessas mídias e de seus websites.

  17. Paul Strand y las paradojas de la modernidad americana

    Directory of Open Access Journals (Sweden)

    Vicente Sánchez Biosca

    2010-06-01

    Full Text Available As contribuições de Strand para o cinema estão estreitamente relacionadas com seus trabalhos fotográficos da época e mesmo com um olhar que, apesar de suas múltiplas diferenças, possui muitos pontos em comum. Se em Manhatta (1920, Strand (em colaboração com Sheeler oferece uma síntese da natureza e da modernidade, em Redes (1934 o que predomina, em contrapartida, é a experiência da arte muralista mexicana e a influência de Eisenstein (em suas andanças por México são os fatores que determinam seus esforços na construção de uma épica dos operários. Mas sem dúvida, o projeto mais ambicioso de Strand (ao mando da produtora Frontier Films foi Native Land, o filme que atendeu às demandas do American Popular Front e as moldou nas palavras dos fundadores da pátria americana.

  18. Dispersal of invasive Phytolacca americana seeds by birds in an urban garden in China.

    Science.gov (United States)

    Li, Ning; Yang, Wen; Fang, Shubo; Li, Xinhai; Liu, Zhanchen; Leng, Xin; An, Shuqing

    2017-01-01

    Although seed dispersal is a key process determining the regeneration and spread of invasive plant populations, few studies have explicitly addressed the link between dispersal vector behavior and seedling recruitment to gain insight into the invasion process within an urban garden context. We evaluated the role of bird vectors in the dispersal of pokeweed (Phytolacca americana), a North American herb that is invasive in urban gardens in China. Fruiting P. americana attracted both generalist and specialist bird species that fed on and dispersed its seeds. The generalist species Pycnonotus sinensis and Urocissa erythrorhyncha were the most frequent dispersers. Seedling numbers of P. americana were strongly associated with the perching behavior of frugivorous birds. If newly recruited bird species use seedling-safe perching sites, the P. americana will regenerate faster, which would enhance its invasive potential. Based on our observations, we conclude that the 2 main bird vectors, P. sinensis and U. erythrorhyncha, provide potential effective dispersal agents for P. americana. Our results highlight the role of native birds in seed dispersal of invasive plants in urban gardens. © 2016 International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.

  19. Susceptibility of Porphyromonas gingivalis and Streptococcus mutans to Antibacterial Effect from Mammea americana

    Directory of Open Access Journals (Sweden)

    Alejandra Herrera Herrera

    2014-01-01

    Full Text Available The development of periodontal disease and dental caries is influenced by several factors, such as microorganisms of bacterial biofilm or commensal bacteria in the mouth. These microorganisms trigger inflammatory and immune responses in the host. Currently, medicinal plants are treatment options for these oral diseases. Mammea americana extracts have reported antimicrobial effects against several microorganisms. Nevertheless, this effect is unknown against oral bacteria. Therefore, the aim of this study was to evaluate the antibacterial effect of M. americana extract against Porphyromonas gingivalis and Streptococcus mutans. For this, an experimental study was conducted. Ethanolic extract was obtained from seeds of M. americana (one oil phase and one ethanolic phase. The strains of Porphyromonas gingivalis ATCC 33277 and Streptococcus mutans ATCC 25175 were exposed to this extract to evaluate its antibacterial effect. Antibacterial activity was observed with the two phases of M. americana extract on P. gingivalis and S. mutans with lower MICs (minimum inhibitory concentration. Also, bactericidal and bacteriostatic activity was detected against S. mutans, depending on the concentration of the extract, while on M. americana extract presented only bacteriostatic activity against P. gingivalis. These findings provide important and promising information allowing for further exploration in the future.

  20. Neuropharmacological and neuroprotective activities of some metabolites produced by cell suspension culture of Waltheria americana Linn.

    Science.gov (United States)

    Mundo, Jorge; Villeda-Hernández, Juana; Herrera-Ruiz, Maribel; Gutiérrez, María Del Carmen; Arellano-García, Jesús; León-Rivera, Ismael; Perea-Arango, Irene

    2017-10-01

    Waltheria americana is a plant used in Mexican traditional medicine to treat some nervous system disorders. The aims of the present study were to isolate and determine the neuropharmacological and neurprotective activities of metabolites produced by a cell suspension culture of Waltheria americana. Submerged cultivation of W. americana cells provided biomass. A methanol-soluble extract (WAsc) was obtained from biomass. WAsc was fractionated yielding the chromatographic fractions 4WAsc-H 2 O and WAsc-CH 2 Cl 2 . For the determination of anticonvulsant activity in vivo, seizures were induced in mice by pentylenetetrazol (PTZ). Neuropharmacological activities (release of gamma amino butyric acid (GABA) and neuroprotection) of chromatographic fractions were determined by in vitro histological analysis of brain sections of mice post mortem. Fraction 4WAsc-H 2 O (containing saccharides) did not produce neuronal damage, neurodegeneration, interstitial tissue edema, astrocytic activation, nor cell death. Pretreatment of animals with 4WAsc-H 2 O and WAsc-CH 2 Cl 2 from W. americana cell suspensions induced an increase in: GABA release, seizure latency, survival time, neuroprotection, and a decrease in the degree of severity of tonic/tonic-clonic convulsions, preventing PTZ-induced death of up to 100% of animals of study. Bioactive compounds produced in suspension cell culture of W. americana produce neuroprotective and neuropharmacological activities associated with the GABAergic neurotransmission system. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  1. Preparation of Eleutherine americana-Alginate Complex Microcapsules and Application in Bifidobacterium longum

    Science.gov (United States)

    Phoem, Atchara N; Chanthachum, Suphitchaya; Voravuthikunchai, Supayang P

    2015-01-01

    Microencapsulation using extrusion and emulsion techniques was prepared for Bifidobacterium longum protection against sequential exposure to simulated gastric and intestinal juices, refrigeration storage and heat treatment. Eleutherine americana was used as the co-encapsulating agent. Hydrolysis of E. americana by gastric and intestinal juices was also determined. E. americana and its oligosaccharide extract demonstrated their resistance to low pH and partial tolerance to human α-amylase. Microencapsulated B. longum with E. americana and oligosaccharide extract prepared by the extrusion technique survived better than that by the emulsion technique under adverse conditions. Survival of microencapsulated cells after exposure to the juices and refrigeration storage was higher than free cells at Weeks 2 and 4. In addition, the viability of microencapsulated cells was better than free cells at 65 °C for 15 min. This work suggested that microencapsulated B. longum with E. americana offers the effective delivery of probiotics to colon and maintains their survival in food products. PMID:25629556

  2. RELACIONES INJERTO-INTERINJERTO Y CARACTERÍSTICAS ANATÓMICAS Y FISIOLÓGICAS DE LA HOJA DE CUATRO GENOTIPOS DE AGUACATE

    Directory of Open Access Journals (Sweden)

    J. Ayala-Arreola

    2010-01-01

    Full Text Available Se evaluó la influencia del interinjerto en las características fisiológicas y anatómicas de la hoja de aguacate 'Hass', 'Fuerte', 'Colín V-33' y 'Duke 7' utilizados como interinjertos e injertos finales. En la lámina foliar se determinó la densidad estomática, densidad de células epidérmicas, índice estomático y diámetro polar del estoma. En cortes transversales de la vena central y del pecíolo de la hoja se evaluó: área, perímetro, longitud del eje mayor y menor, índice de redondez y diámetro Feret en vasos del xilema. En cortes transversales de hoja, se evaluó: el grosor de la epidermis superior e inferior y del parénquima en empalizada I. Las variables fisiológicas evaluadas fueron: tasa de asimilación de CO2, conductancia estomática, concentración interna de CO2, resistencia estomática, tasa de transpiración y temperatura de la hoja. 'Colín V-33' como interinjerto promovió la mayor densidad estomática, mayor densidad de células epidérmicas y grosor de la capa de parénquima en empalizada I. En la comparación intra genotipos se encontró que 'Hass' y 'Fuerte' con interinjerto incrementaron en 18.8 y 17.8 % el número de estomas, mientras que en 'Duke 7' disminuyó en un 25.4 %. La densidad de células epidérmicas se incrementó de manera significativa en 'Fuerte' (23.3 % con la presencia del interinjerto. 'Fuerte' y 'Duke 7' con interinjerto incrementaron significativamente el grosor de la epidermis inferior en 14.9 y 12.1 %, respectivamente, mientras que 'Duke 7', 'Hass' y 'Colín V-33' con interinjerto disminuyeron el grosor de epidermis inferior en 19.5 y 1.0 %. Se encontró que 'Hass' como interinjerto promovió la tasa de asimilación neta de CO2 más alta, siendo diferente 'Duke 7'. Para resistencia estomática le correspondió el menor valor a 'Colín V-33' y presentó diferencias con 'Duke 7'.

  3. Orchidaceae Americanae Orchidaceae Americanae

    Directory of Open Access Journals (Sweden)

    Jones H. G.

    1970-09-01

    Full Text Available The following paper continues the series of nomenclatural notes and adjustments commenced under the title "Studies in Neotropical Orchidology" in Acta Botanica Academiae Scientiarum Hungaricae, Vol. 14: pp. 63-70 (1968. As in the previous contribution, the genera considered in the present study are Brassavola R. Br., Schomburgkia Lindl, and HoffmannseggellaH. G. Jones. The notes which comprise this series of studieshave been accumulated during the course of collecting materials for a taxonomic revision of the three genera concerned.The following paper continues the series of nomenclatural notes and adjustments commenced under the title "Studies in Neotropical Orchidology" in Acta Botanica Academiae Scientiarum Hungaricae, Vol. 14: pp. 63-70 (1968. As in the previous contribution, the genera considered in the present study are Brassavola R. Br., Schomburgkia Lindl, and HoffmannseggellaH. G. Jones. The notes which comprise this series of studieshave been accumulated during the course of collecting materials for a taxonomic revision of the three genera concerned.

  4. Initial evaluation of fruit of accessions of Persea schiedeana Nees for nutritional value, quality and oil extraction.

    Science.gov (United States)

    López-Yerena, A; Guerra-Ramírez, D; Jácome-Rincón, J; Espinosa-Solares, T; Reyes-Trejo, B; Famiani, F; Cruz-Castillo, J G

    2018-04-15

    Persea schiedeana Nees is an underutilized and very little known species whose fruit is consumed in Mesoamerica where it grows wild. This study was carried out to evaluate: 1) the variability of fruit characteristics of different accessions; 2) the effects of centrifugation and microwave treatment on extracting oil from the fruit and on its qualitative characteristics; 3) the nutraceutical characteristics of the fruit and seeds of different accessions. The results showed a large variability in fruit size and oil/dry matter contents among the different accessions. There was a significant relationship between the dry matter and oil contents in the pulp. The combined use of centrifugation and microwave treatments gave high oil extraction yields (67-68%). The oils had good fatty acid composition and antioxidant capacity. The results gave an initial picture about the total phenol contents and antioxidant capacities in the seeds and in the different parts of the fruit. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Identification of rosmarinic acid as the major active constituent in Cordia americana.

    Science.gov (United States)

    Geller, F; Schmidt, C; Göttert, M; Fronza, M; Schattel, V; Heinzmann, B; Werz, O; Flores, E M M; Merfort, I; Laufer, S

    2010-04-21

    Preparation from leaves of Cordia americana have been widely used in traditional medicine in South Brazil to treat wounds and various inflammations. The objective of this work was to identify the effective compounds in the ethanolic extract prepared from the leaves of Cordia americana, which is used in traditional South Brazilian medicine as anti-inflammatory and wound healing remedy. Isolation and structure elucidation techniques were performed in order to identify the compounds of Cordia americana and HPLC analysis was used for the quantification. The major constituent and the ethanolic extract were investigated for inhibition of 5-lipoxygenase, p38alpha MAPK, TNFalpha release and NF-kappaB as well as in the fibroblast scratch assay. Rosmarinic acid (1) was identified as the major compound with an amount of 8.44% in the ethanolic extract of the leaves of Cordia americana. The ethanolic extract as well as (1) exhibited the highest inhibitory effects on 5-lipoxygenase (IC(50)=0.69 and 0.97microg/mL, resp., IC50 of BWA4C as reference: 0.3microM) and p38alpha (IC50=3.25 and 1.16microg/mL, resp., IC50 of SB203580 as reference: 0.046microM) and moderate inhibitory effects on TNFalpha release. Slight effects were observed in the fibroblast scratch assay. This study increases our knowledge on the effective compound in Cordia americana and supports its use in traditional medicine. We demonstrated for the first time pharmacological effects of Cordia americana and we provide evidences for a crucial role of rosmarinic acid as the major key player. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

  6. Evaluation of fruit productivity and quality in Hass avocado submitted to {sup 60}Co gamma radiation; Evaluacion de productividad y calidad de fruta en aguacate Hass sometido a radiacion gamma de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Cruz T, E. De la; Ibanez P, J; Mijares O, P; Garcia A, J M [Instituto nacional de Investigaciones Nucleares, Departamento de Biologia, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1999-07-01

    Evaluation of productivity, postharvest behavior and fruit quality was performed on four years Hass avocado trees irradiated with {sup 60} Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the ''La Labor'' Experimental Center of the Centro de Investigaciones Cientificas y Tecnologias del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300 %. In regard to postharvest performance, the respiration index (mg CO{sub 2}/kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This mean that radiation has altered productivity but not the quality and postharvest behavior of fruits. (Author)

  7. Zonas hortofrutícolas emergentes en México. ¿Viabilidad de largo plazo o coyuntura de corto plazo?. La producción de aguacate en el sur de Jalisco

    Directory of Open Access Journals (Sweden)

    Alejandro Macías Macías

    2010-01-01

    Full Text Available El aguacate es el cultivo que mayor dinamismo registra en el sur del estado de Jalisco en la primera década del siglo XXI. Ello corresponde a los cambios que se vienen dando en la relación comercial entre México y los Estados Unidos, así como a la cercanía de la región con Michoacán, principal zona productora. Esta actividad económica, que se presenta como potencial detonadora de la economía regional, forma parte de un modelo agroindustrial que en aras de la rentabilidad económica, puede resultar depredador de los recursos locales e incrementar los niveles de vulnerabilidad de los territorio de producción, tanto desde el punto de vista económico, como medioambiental.

  8. A REPRESENTAÇÃO DA MULHER LATINO-AMERICANA: DA LITERATURA PARA O CINEMA

    OpenAIRE

    de Amorim, Maria Inês Freitas; Universidade do Estado do Rio de Janeiro

    2015-01-01

    As obras do escritor colombiano Gabriel García Márquez são reconhecidas pela presença de personagens femininas fortes e pela representação de elementos da cultura latino-americana. Já a indústria cinematográfica de Hollywood costuma representar a mulher latino-americana a partir de estereótipos, sobretudo relacionados à sensualidade e submissas às vontades da voz masculina. O romance de García Márquez El amor en los tiempos del cólera foi adaptado para o cinema pela industria estadunidense, ...

  9. Polysaccharide rich fractions from barks of Ximenia americana inhibit peripheral inflammatory nociception in mice Antinociceptive effect of Ximenia americana polysaccharide rich fractions

    Directory of Open Access Journals (Sweden)

    Kaira E.S. da Silva-Leite

    Full Text Available Abstract Ximenia americana L., Olacaceae, barks are utilized in folk medicine as analgesic and anti-inflammatory. The objective was to evaluate the toxicity and antinociceptive effect of polysaccharides rich fractions from X. americana barks. The fractions were obtained by extraction with NaOH, followed by precipitation with ethanol and fractionation by ion exchange chromatography. They were administered i.v. or p.o. before nociception tests (writhing, formalin, carragenan-induced hypernociception, hot plate, or during 14 days for toxicity assay. The total polysaccharides fraction (TPL-Xa: 8.1% yield presented 43% carbohydrate (21% uronic acid and resulted in two main fractions after chromatography (FI: 12%, FII: 22% yield. FII showed better homogeneity/purity, content of 44% carbohydrate, including 39% uronic acid, arabinose and galactose as major monosaccharides, and infrared spectra with peaks in carbohydrate range for COO- groups of uronic acid. TPL-Xa (10 mg/kg and FII (0.1 and 1 mg/kg presented inhibitory effect in behavior tests that evaluate nociception induced by chemical and mechanical, but not thermal stimuli. TPL-Xa did not alter parameters of systemic toxicity. In conclusion, polysaccharides rich fractions of X. americana barks inhibit peripheral inflammatory nociception, being well tolerated by animals.

  10. Aktivitas Ekstrak Etanol Daun Alpukat Terhadap Zat Nefrotoksik Ginjal Tikus (ACTIVITIES STUDY OF ETHANOL EXTRACTS OF AVOCADO LEAVES (PERSEA AMERICANA MILL TO NEPHROTOXIC COMPOUND OF RAT’S KIDNEY

    Directory of Open Access Journals (Sweden)

    Ietje Wientarsih

    2014-08-01

    Full Text Available Based on WHO’s data, about 80% peoples in the word use plant’s extract as herbal medicine. Avocadoleaves has used as herbal medicine which is works as diuretikum, reduce stone in renal, and cure sprue.The purpose of this research is to study activities of ethanol extract of avocado leaves to ethylene glycolinduction on rat’s kidney by histopatologically. This experiment was continuing Adha’s research (2009with research design as follows: twenty male rats were divided into 4 groups, negative control group (KN,positive control group (KP, treated group I by ethanol extract of avocado leaves 100 mg/kg BW (E100, andtreated group 2 by 300 mg/kg BW (E300. Sampling kidney were fixed in BNF 10% for histopathologicalslide and stained by Hematoxylin-Eosin. The kidney histopathological changes were edema of glomerulus,and protein deposit in the lumen, hyalin droplet and necrotic tubules. The results showed that ethanolextract of avocado leaves can’t significantly to reduce glomerulus edema with percentage cases 31.9% and33.5% for E100 and E300 and tend not to reduce the occurrence of protein deposits in the lumen of thetubule. Ethanol extract of avocado leaves tend to reduce the incidence of tubular necrosis and it wassignificantly different from the KP group (p <0.05. It is caused by flavonoid in etanol extract of avocadoleaves works as diuretikum and antioxidant.

  11. Integrating transient heterogeneity of non-photochemical quenching in shade-grown heterobaric leaves of avocado (Persea americana L.): responses to CO2 concentration, stomatal occlusion, dehydration and relative humidity.

    Science.gov (United States)

    Takayama, Kotaro; King, Diana; Robinson, Sharon A; Osmond, Barry

    2013-11-01

    Long-lived shade leaves of avocado had extremely low rates of photosynthesis. Gas exchange measurements of photosynthesis were of limited use, so we resorted to Chl fluorescence imaging (CFI) and spot measurements to evaluate photosynthetic electron transport rates (ETRs) and non-photochemical quenching (NPQ). Imaging revealed a remarkable transient heterogeneity of NPQ during photosynthetic induction in these hypostomatous, heterobaric leaves, but was adequately integrated by spot measurements, despite long-lasting artifacts from repeated saturating flashes during assays. Major veins (mid-vein, first- and second-order veins) defined areas of more static large-scale heterogeneous NPQ, with more dynamic small-scale heterogeneity most strongly expressed in mesophyll cells between third- and fourth-order veins. Both responded to external CO2 concentration ([CO2]), occlusion of stomata with Vaseline™, leaf dehydration and relative humidity (RH). We interpreted these responses in terms of independent behavior of stomata in adjacent areoles that was largely expressed through CO2-limited photosynthesis. Heterogeneity was most pronounced and prolonged in the absence of net CO2 fixation in 100 p.p.m. [CO2] when respiratory and photorespiratory CO2 cycling constrained the inferred ETR to ~75% of values in 400 or 700 p.p.m. [CO2]. Likewise, sustained higher NPQ under Vaseline™, after dehydration or at low RH, also restricted ETR to ~75% of control values. Low NPQ in chloroplast-containing cells adjacent to major veins but remote from stomata suggested internal sources of high [CO2] in these tissues.

  12. White ash (Fraxinus americana) decline and mortality: the role of site nutrition and stress history

    Science.gov (United States)

    Alejandro A. Royo; Kathleen S. Knight

    2012-01-01

    Over the past century, white ash (Fraxinus americana) populations throughout its range have deteriorated as a result of declining tree health and increased mortality rates. Although co-occurring factors including site nutritional deficiencies and punctuated stress events (e.g., defoliations, drought) are hypothesized to trigger white ash decline,...

  13. Multi scale habitat relationships of Martes americana in northern Idaho, U.S.A.

    Science.gov (United States)

    Tzeidle N. Wasserman; Samuel A. Cushman; David O. Wallin; Jim Hayden

    2012-01-01

    We used bivariate scaling and logistic regression to investigate multiple-scale habitat selection by American marten (Martes americana). Bivariate scaling reveals dramatic differences in the apparent nature and strength of relationships between marten occupancy and a number of habitat variables across a range of spatial scales. These differences include reversals in...

  14. Delineation of a New Species of the Borrelia burgdorferi Sensu Lato Complex, Borrelia americana sp. nov

    Czech Academy of Sciences Publication Activity Database

    Rudenko, Natalia; Golovchenko, Maryna; Lin, T.; Gao, L.; Grubhoffer, Libor; Oliver, J. H., Jr.

    2009-01-01

    Roč. 47, č. 12 (2009), s. 3875-3880 ISSN 0095-1137 R&D Projects: GA MŠk(CZ) LC06009 Institutional research plan: CEZ:AV0Z60220518 Keywords : multilocus sequence analysis * B. burgdorferi sl complex * new borrelia species * Borrelia americana Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 4.162, year: 2009

  15. Developmental ecdysteroid titers and DNA puffs in larvae of two sciarid species, Rhynchosciara americana and Rhynchosciara milleri (Diptera: Sciaridae).

    Science.gov (United States)

    Soares, M A M; Hartfelder, K; Tesserolli de Souza, J M; Stocker, A J

    2015-10-01

    Ecdysteroid titers, developmental landmarks and the presence of prominent amplifying regions (DNA puffs) have been compared during late larval to pupal development in four groups of Rhynchosciara americana larvae and in R. americana and Rhynchosciara milleri. Three prominent DNA puffs (B2, C3 and C8) expand and regress sequentially on the rising phase of the 20-hydroxyecdysone (20E) titer in R. americana as a firm, cellular cocoon is being constructed. A sharp rise in 20E coincides with the regression of these puffs. The shape of the 20E curve is similar in R. milleri, a species that does not construct a massive cocoon, but the behavior of certain DNA puffs and their temporal relationship to the curve differs. Regions corresponding to B2 and C3 can be identified in R. milleri by banding pattern similarity with R. americana chromosomes and, in the case of B2, by hybridization to an R. americana probe. A B2 puff appears in R. milleri as the 20E titer rises but remains small in all gland regions. A puff similar to the R. americana C3 puff occurs in posterior gland cells of R. milleri (C3(Rm)) after the B2 puff, but this site did not hybridize to R. americana C3 probes. C3(Rm) incorporated (3)H-thymidine above background, but showed less post-puff DNA accumulation than C3 of R. americana. R. americana C8 probes hybridized to a more distal region of the R. milleri C chromosome that did not appear to amplify or form a large puff. These differences can be related to developmental differences, in particular differences in cocoon construction between the two species.

  16. Evaluation of Piper aduncum Linn. Essential Oil (Fam:Piperaceae against Periplaneta americana(L.

    Directory of Open Access Journals (Sweden)

    I Ling A

    2009-12-01

    Full Text Available Background: The efficacy of Piper aduncum essential oil was evaluated against Periplaneta americana adults and nymphs in the laboratory.Methods: The plant essential oil at varying concentrations ranging between 10,000 to 80,000 ppm were placed inside glass beakers, rolled horizontally to ensure the essential oil covers all sides of the beakers and exposed to adults and nymphs of P. americana. Resigen (R 1ppm was used as positive control and distilled water as negative control. The LT50 and LT90 was obtained using Log Probit programme.Results: Exposure of essential oil to females P. americana at concentrations between 10,000 to 80,000 ppm indicated the LT50 and LT90 values between 5.31 h-189.19 h and 14.90 h-2105.31 h, respectively. Treatment with the same con­centrations against males P. americana ,the LT50 and LT90 were 2.08 h-181.73 h and 5.4 h-8460.51 h, respectively. Treatment against the nymphal stage with the same range of concentrations indicated the LT50 and LT 90 of 4.68 h-381.02 h and 28.71 h-5313.36 h, respectively.The nymphs and males were more susceptible than the females cock­roaches. Treatment with Resigen (R at 1ppm indicated much lower LT 50 and LT 90 values of 2.54 h-9.47 h for the fe­males, 1.47 h-4.22 h for the males and 4.69 h-8.92 h for the nymphs.The negative control indicated no mortality for all stages of the cockroach.Conclusion: Piper aduncum essential oil can be used as an alternative natural product for controlling the cockroach Peri­patetic americana.

  17. Evaluation of Piper aduncum Linn. Essential Oil (Fam:Piperaceae) against Periplaneta americana (L.).

    Science.gov (United States)

    Ling A, I; Sulaiman, S; Othman, H

    2009-01-01

    The efficacy of Piper aduncum essential oil was evaluated against Periplaneta americana adults and nymphs in the laboratory. The plant essential oil at varying concentrations ranging between 10,000 to 80,000 ppm were placed inside glass beakers, rolled horizontally to ensure the essential oil covers all sides of the beakers and exposed to adults and nymphs of P. americana. Resigen (R) 1ppm was used as positive control and distilled water as negative control. The LT50 and LT90 was obtained using Log Probit programme. Exposure of essential oil to females P. americana at concentrations between 10,000 to 80,000 ppm indicated the LT50 and LT90 values between 5.31 h-189.19 h and 14.90 h-2105.31 h, respectively. Treatment with the same concentrations against males P. americana ,the LT50 and LT90 were 2.08 h-181.73 h and 5.4 h-8460.51 h, respectively. Treatment against the nymphal stage with the same range of concentrations indicated the LT50 and LT 90 of 4.68 h-381.02 h and 28.71 h-5313.36 h, respectively.The nymphs and males were more susceptible than the females cockroaches. Treatment with Resigen (R) at 1ppm indicated much lower LT 50 and LT 90 values of 2.54 h-9.47 h for the females, 1.47 h-4.22 h for the males and 4.69 h-8.92 h for the nymphs.The negative control indicated no mortality for all stages of the cockroach. Piper aduncum essential oil can be used as an alternative natural product for controlling the cockroach Peripatetic americana.

  18. TECNOLOGÍA DE ATMÓSFERAS CONTROLADAS PARA FRUTAS Y HORTALIZAS, CASOTecnología de atmósferas controladas para frutas y hortalizas, caso de estudio: modelo aguacate (perseamericana variedad HASS

    Directory of Open Access Journals (Sweden)

    Diego Montoya

    2007-05-01

    Full Text Available The controlled atmosphere technology (AC is used in cold quarters for the storage, or in containers for the transport, of vegetables and fruits products. This technique stabilizes the atmosphere that surrounds the product in precise percentage with relation to the present O2 and CO2 in her, depending on the vegetal variety to control. For the avocado (American Persea like climacterical fruit and concretely for the Hass variety, product of world-wide export, the conservation in AC is innovating for the maintenance of its optimal quality.

  19. Evaluation of Piper aduncum Linn. Essential Oil (Fam:Piperaceae against Periplaneta americana(L.

    Directory of Open Access Journals (Sweden)

    I Ling A

    2009-12-01

    Full Text Available Background: The efficacy of Piper aduncum essential oil was evaluated against Periplaneta americana adults and nymphs in the laboratory."nMethods: The plant essential oil at varying concentrations ranging between 10,000 to 80,000 ppm were placed inside glass beakers, rolled horizontally to ensure the essential oil covers all sides of the beakers and exposed to adults and nymphs of P. americana. Resigen (R 1ppm was used as positive control and distilled water as negative control. The LT50 and LT90 was obtained using Log Probit programme."nResults: Exposure of essential oil to females P. americana at concentrations between 10,000 to 80,000 ppm indicated the LT50 and LT90 values between 5.31 h-189.19 h and 14.90 h-2105.31 h, respectively. Treatment with the same con­centrations against males P. americana ,the LT50 and LT90 were 2.08 h-181.73 h and 5.4 h-8460.51 h, respectively. Treatment against the nymphal stage with the same range of concentrations indicated the LT50 and LT 90 of 4.68 h-381.02 h and 28.71 h-5313.36 h, respectively.The nymphs and males were more susceptible than the females cock­roaches. Treatment with Resigen (R at 1ppm indicated much lower LT 50 and LT 90 values of 2.54 h-9.47 h for the fe­males, 1.47 h-4.22 h for the males and 4.69 h-8.92 h for the nymphs.The negative control indicated no mortality for all stages of the cockroach."nConclusion: Piper aduncum essential oil can be used as an alternative natural product for controlling the cockroach Peri­patetic americana. "nKeywords: Piper aduncum essential oil, Periplaneta americana, Adults, Nymphs

  20. UN HELICTERES NUEVO DE MEXICO, NEXO ENTRE LAS ESPECIES ASIATICAS y AMERICANAS

    Directory of Open Access Journals (Sweden)

    Carmen L. Cristóbal

    1987-01-01

    Full Text Available Una nueva especie, Helicteres vegae (Sterculiaceae, de Sinaloa, México es descripta e ilustrada. Se encontró el número de cromosomas 2n = 18. H. vegae se compara con el resto de las especies mexicanas y se proporciona una clave. Su afinidad con la de Asia y las especies americanas es discutida. H. vegae junto con H. Rekoí y H.carthagenensis se consideran las más diferenciadas de las especies americanas. Se propone una posible área de origen de América para Helicteres , incluido en esta área se encuentran los otros dos nuevos géneros mundiales de la tribu Helictereae.

  1. Applications of Microencapsulated Bifidobacterium Longum with Eleutherine Americana in Fresh Milk Tofu and Pineapple Juice

    Science.gov (United States)

    Phoem, Atchara N.; Chanthachum, Suphitchaya; Voravuthikunchai, Supayang P.

    2015-01-01

    Bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. Microencapsulation of B. longum with Eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. Microencapsulated B. longum with the extract and oligosaccharides extract in the food products showed better survival than free cells under adverse conditions. Sensory analysis demonstrated that the products containing co-encapsulated bacterial cells were more acceptable by consumers than free cells. Pineapple juice prepared with co-encapsulated cells had lower values for over acidification, compared with the juice with free cells added. This work suggested that microencapsulated B. longum with E. americana could enhance functional properties of fresh milk tofu and pineapple juice. PMID:25854832

  2. Applications of Microencapsulated Bifidobacterium Longum with Eleutherine Americana in Fresh Milk Tofu and Pineapple Juice

    Directory of Open Access Journals (Sweden)

    Atchara N. Phoem

    2015-04-01

    Full Text Available Bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. Microencapsulation of B. longum with Eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. Microencapsulated B. longum with the extract and oligosaccharides extract in the food products showed better survival than free cells under adverse conditions. Sensory analysis demonstrated that the products containing co-encapsulated bacterial cells were more acceptable by consumers than free cells. Pineapple juice prepared with co-encapsulated cells had lower values for over acidification, compared with the juice with free cells added. This work suggested that microencapsulated B. longum with E. americana could enhance functional properties of fresh milk tofu and pineapple juice.

  3. Pathogenicity of Aspergillus westerdijkiae to females and oothecae of Periplaneta Americana

    Directory of Open Access Journals (Sweden)

    Mariah Valente Baggio

    2016-01-01

    Full Text Available ABSTRACT: Cockroach control is performed by the application of chemical insecticides which exert high selective pressure on populations and introduces synthetic substances in the environment, motivating the search for other methods of control such as entomopathogenic fungi. The objectives of this study were to investigate the pathogenicity of the JAB 42 Aspergillus westerdijkiae to females and oothecae of Periplaneta americana and to demonstrate its mechanism of action on oothecae. Suspensions containing 106 to 108 conidia/ml were used to infect females and oothecae. Mortality and other variables such as scanning electron microscopy were used to demonstrate the mechanism of action of the fungus. The isolated JAB 42 A. westerdijkiae is pathogenic to oothecae of P. americana, with low capacity to kill females. Adhesion, germination, penetration and extrusion of the fungus on the cockroach oothecae were observed.

  4. Manganese uptake and interactions with cadmium in the hyperaccumulator-Phytolacca Americana L

    Energy Technology Data Exchange (ETDEWEB)

    Peng Kejian [College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 (China); Hunan Research Academy of Environmental Sciences Changsha 410004 (China); Luo Chunling [Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon (Hong Kong); You Wuxin [College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 (China); Lian Chunlan [Asian Natural Environmental Science Center, University of Tokyo, 1-1-8 Midori-cho, Nishitokyo, Tokyo 188-0002 (Japan); Li Xiangdong [Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon (Hong Kong); Shen Zhenguo [College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 (China)], E-mail: zgshen@njau.edu.cn

    2008-06-15

    In the present study, the accumulation of Mn and other metals by Phytolacca Americana L. from contaminated soils in Hunan Province, South China, was investigated. Results showed that the average concentrations of Mn in the leaves and roots reached 2198 and 80.4 mg kg{sup -1} (dry weight), respectively, with a maximum 13,400 mg kg{sup -1} in the leaves. A significant correlation was found between Mn concentrations in the plant leaves and those in the corresponding soils. Hydroponic experiments were also conducted to study the Cd uptake ability and interactions between Mn and Cd in the plant. It was found that P. americana hyperaccumulated not only Mn, but also Cd in the leaves. In the presence of Cd, adding Mn to the solution significantly improved the plant growth and reduced the concentrations of Cd in all organs of the plant.

  5. Identification of Heterobilharzia americana infection in a dog residing in Indiana with no history of travel.

    Science.gov (United States)

    Rodriguez, Jessica Y; Camp, Joseph W; Lenz, Stephen D; Kazacos, Kevin R; Snowden, Karen F

    2016-04-01

    A 1-year-old castrated male dog residing in Indiana was examined because of intermittent vomiting of 4 months' duration. The dog's condition did not resolve with medication. Diagnostic imaging revealed a possible partial obstruction at the ileocecal junction. An exploratory laparotomy was performed. The jejunum contained diffusely distributed, nodular, intramural lesions; 2 biopsy specimens were collected from representative lesions. The pancreas was grossly swollen, and pancreatitis was presumed present. No other abnormalities were observed in the abdomen. Histologic examination of the submitted biopsy specimens revealed infection with Heterobilharzia americana. After diagnosis, the dog was treated with fenbendazole suspension (48 mg/kg [21.8 mg/lb], PO, q 24 h) for 10 days. This treatment was subsequently repeated 11 and 80 days later. One week after the end of the last fenbendazole treatment, several H americana eggs were detected in a fecal sample via saline sedimentation, and the dog was given praziquantel (25 mg/kg [11.4 mg/lb], PO, q 8 h) for 2 days. No gastrointestinal signs were evident 4 months after that treatment. The dog described in this report was the first autochthonous canine case of H americana infection in Indiana, to the authors' knowledge; this case has confirmed that the distribution of this parasite in the Midwestern United States is broader than previously known. Increased awareness of the distribution of H americana should aid veterinarians in early, noninvasive diagnosis and appropriate treatment of affected animals. Repeated treatments and recheck fecal examinations may be necessary when managing these cases.

  6. One pot phytosynthesis of gold nanoparticles using Genipa americana fruit extract and its biological applications

    International Nuclear Information System (INIS)

    Kumar, Brajesh; Smita, Kumari; Cumbal, Luis; Camacho, Javier; Hernández-Gallegos, Elisabeth; Guadalupe Chávez-López, María de; Grijalva, Marcelo; Andrade, Kleber

    2016-01-01

    In this article, rapid one pot synthesis of gold nanoparticles (GNPs) using an eco-friendly extract of Genipa americana L. fruit is described. Electrospray ionization mass spectrometry (ESI-MS) and Fourier transform infrared (FTIR) spectroscopic studies demonstrated that small molecules such as genipin, genipaol, geniposide and ranolazine can act as reducer as well as stabilizers. The monodispersed, spherical GNPs were further characterized by UV–vis spectroscopy at λ max = 535 nm, transmission electron microscopy (TEM), dynamic light scattering (DLS) and X-ray diffraction (XRD) analysis. This synthetic approach offers a greener and alternate route to the preparation of GNPs free from toxic chemical components and stable for 6–7 months under room temperature. The green synthesized GNPs showed weak antioxidant efficacy against 1,1-diphenyl-2-picrylhydrazyl and no cytotoxicity against A-549 and HeLa human cancer cell lines, from lung and cervix. This study opens a new industrial scope of G. americana fruit in nanoscience and as surface modified GNPs can be developed into a successful drug carrier for future pharmaceutical products. - Highlights: • Gold nanoparticles can be synthesized by Genipa americana fruit extract. • TEM-DLS analysis confirmed that the average particle size is 30.4 ± 14.9 nm. • Nanoparticles showed weak antioxidant and no cytotoxicity activity.

  7. One pot phytosynthesis of gold nanoparticles using Genipa americana fruit extract and its biological applications

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Brajesh, E-mail: krmbraj@gmail.com [Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B (Ecuador); Smita, Kumari; Cumbal, Luis [Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B (Ecuador); Camacho, Javier [Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B (Ecuador); Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Farmacología, C.P. 07360 Mexico City (Mexico); Hernández-Gallegos, Elisabeth; Guadalupe Chávez-López, María de [Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Farmacología, C.P. 07360 Mexico City (Mexico); Grijalva, Marcelo; Andrade, Kleber [Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B (Ecuador)

    2016-05-01

    In this article, rapid one pot synthesis of gold nanoparticles (GNPs) using an eco-friendly extract of Genipa americana L. fruit is described. Electrospray ionization mass spectrometry (ESI-MS) and Fourier transform infrared (FTIR) spectroscopic studies demonstrated that small molecules such as genipin, genipaol, geniposide and ranolazine can act as reducer as well as stabilizers. The monodispersed, spherical GNPs were further characterized by UV–vis spectroscopy at λ{sub max} = 535 nm, transmission electron microscopy (TEM), dynamic light scattering (DLS) and X-ray diffraction (XRD) analysis. This synthetic approach offers a greener and alternate route to the preparation of GNPs free from toxic chemical components and stable for 6–7 months under room temperature. The green synthesized GNPs showed weak antioxidant efficacy against 1,1-diphenyl-2-picrylhydrazyl and no cytotoxicity against A-549 and HeLa human cancer cell lines, from lung and cervix. This study opens a new industrial scope of G. americana fruit in nanoscience and as surface modified GNPs can be developed into a successful drug carrier for future pharmaceutical products. - Highlights: • Gold nanoparticles can be synthesized by Genipa americana fruit extract. • TEM-DLS analysis confirmed that the average particle size is 30.4 ± 14.9 nm. • Nanoparticles showed weak antioxidant and no cytotoxicity activity.

  8. Regional Guidebook for Applying the Hydrogeomorphic Approach to Assessing the Functions of Headwater Slope Wetlands on the Mississippi and Alabama Coastal Plans

    Science.gov (United States)

    2007-08-01

    sourwood Bignonia capreolata crossvine Panicum virgatum switchgrass Callicarpa americana American beautyberry Persea borbonia redbay Calystegia sepium...sweetbay (Magnolia virginiana), loblolly-bay (Gordonia lasianthus), redbay ( Persea borbonia), and swamp bay ( Persea palustris) make up a significant...com- munity model. The Society of American Foresters (Eyre 1980) recognizes a “Sweetbay- Swamp Tupelo (Nyssa sylvatica var. biflora)-Redbay” forest

  9. Assessing Hydraulic Modifications on Vallisneria americana in Peoria Lake, Illinois. A Pilot Study Using Data Sharing Protocols to Integrate Legacy Models

    National Research Council Canada - National Science Library

    Black, Patrick

    2003-01-01

    .... The modeling was directed toward an investigation of potential population increase of the submersed macrophyte Vallisneila americana as a consequence of the construction of a hypothetical levee...

  10. Evaluation of fruit productivity and quality in Hass avocado submitted to {sup 60}Co gamma radiation; Evaluacion de productividad y calidad de fruta en aguacate Hass sometido a radiacion gamma de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Cruz T, E. De la; Ibanez P, J.; Mijares O, P.; Garcia A, J.M. [Instituto nacional de Investigaciones Nucleares, Departamento de Biologia, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1999-07-01

    Evaluation of productivity, postharvest behavior and fruit quality was performed on four years Hass avocado trees irradiated with {sup 60} Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the ''La Labor'' Experimental Center of the Centro de Investigaciones Cientificas y Tecnologias del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300 %. In regard to postharvest performance, the respiration index (mg CO{sub 2}/kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This mean that radiation has altered productivity but not the quality and postharvest behavior of fruits. (Author)

  11. Dicty_cDB: Contig-U03662-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available cultivar Nabal_1 serine-threonin... 48 0.88 1 ( EU335716 ) Persea americana cultivar Mexicola_2 serine-threo...... 48 0.88 1 ( EU335715 ) Persea americana cultivar Mexicola_1 serine-threo... 48 0.88 1 ( EU335714 ) Perse

  12. Morfoanatomia de folha e caule de Genipa americana L., Rubiaceae Leaf and stem morpho-anatomy of Genipa americana L., Rubiaceae

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    Marianna Erbano

    2010-12-01

    Full Text Available Genipa americana L., conhecida como jenipapo, é uma Rubiaceae nativa do Brasil e as suas folhas são utilizadas pela população como antidiarreico e febrífugo, e o caule como anti-hemorrágico, contra luxações e contusões. Com o objetivo de caracterizar macro e microscopicamente folhas adultas e caules jovens para o controle de qualidade farmacognóstico, essa planta medicinal foi analisada segundo a metodologia clássica de morfoanatomia vegetal. Genipa americana apresenta folhas simples, opostas, de formato obovado a oblanceolado e pecíolos curtos. A epiderme foliar é uniestratificada e revestida por cutícula delgada e levemente estriada. Os estômatos são paracíticos e restritos à superfície abaxial. Há tricomas tectores uni e pluricelulares predominantemente na superfície abaxial. O mesofilo é dorsiventral. Em secção transversal, a nervura central é plano-convexa e o pecíolo é circular, ambos apresentando um feixe vascular colateral em disposição cilíndrica e outros menores. O caule possui felogênio localizado nas camadas subepidérmicas, colênquima anelar e um cilindro floemático externo ao de xilema, sendo ambos percorridos por raios parenquimáticos estreitos. Ocorrem idioblastos contendo compostos fenólicos, areia cristalina e drusas de oxalato de cálcio na folha e no caule.Genipa americana L., known as jenipapo, is a Rubiaceae species native to Brazil. Its leaves are used by the population as anti-diarrheic and febrifuge, and its stem as anti-hemorrhagic and for trauma injuries. Aiming to identify macro and microscopically mature leaves and young stems for the pharmacognostic quality control, this medicinal plant was investigated according to standard morpho-anatomical techniques. The leaves are simple, opposite, obovate-oblanceolate, presenting short petiole. The epidermis is uniseriate and coated with thin and slightly striate cuticle. The stomata are paracytic and restricted to the abaxial surface. It

  13. Herança e ligação em locos de isoenzimas em Genipa americana L. Inheritance and linkage in isozymes loci of Genipa americana L.

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    Alexandre Magno SEBBENN

    2004-06-01

    Full Text Available A herança e a ligação foram estudadasem quatro locos polimórficos (6pghd-1, Pgi-2,Mdh-1 e Mdh-2 de três sistemas isoenzimáticos deGenipa americana, analisados por eletroforesehorizontal em gel de amido, usando tecidoscoletados de quinze progênies de polinização aberta,procedentes de Moji-Guaçu, Estado de São Paulo.Esses locos segregaram de dois a três alelos.A segregação nesses locos foi homogênea entreárvores. Contudo, existem desvios significativos dasegregação esperada 1:1 para algumas árvores emalguns locos. Desvios significativos da razão desegregação esperada 1:1 foram detectados noslocos Mdh-2 e 6pgdh-1. Não foram detectadasevidências de ligação entre nenhum dos pares delocos avaliados em G. americana e esses podemser utilizados sem restrição para estudos do sistemade reprodução, diversidade e estrutura genética depopulações da espécie.Inheritance and linkage for fourpolymorphic loci (6pghd-1, Pgi-2, Mdh-1 andMdh-2 from three enzyme systems in Genipaamericana were analyzed by horizontal starch gelelectrophoresis using tissues collected from fiftyopen-pollinated families in Moji-Guaçu, São PauloState. These allozyme loci have segregated fromtwo to four alleles per polymorphic locus. Genesegregation on those loci was homogeneous amongtrees. However, there were significant deviationsfrom the expected segregation ratios on some lociand some individual trees. In addition, Mdh-2 and6pgdh-1 loci have shown significant deviationsfrom the 1:1 expected segregation ratio. Linkagedisequilibrium evidences were not detected betweenany two pairs of loci from G. americana and thosecan be used with no restriction to study the matingsystem, genetic diversity, and genetic structure onthe species.

  14. Estudio de factibilidad para la producción de aceite vegetal comestible de aguacate producido en la finca "Cuatro Hermanos" de la provincia de Manabí y propuesta de plan de exportación dirigido al mercado de Londres, Reino Unido.

    OpenAIRE

    Gómez Zambrano, Karem Lissette

    2015-01-01

    Se realizó un estudio de factibilidad para exportar aceite de aguacate a Londres, Reino Unido producido en la Finca “Cuatro Hermanos” de la provincia de Manabí. Comprobando que la finca cuenta con la capacidad productiva para satisfacer la demanda de 250 toneladas. La tasa interna de retorno fue 29.93%, demostrando que el proyecto es viable. A feasibility study was made to export edible vegetable oil avocado to London, United of Kingdom produced in the farm "Cuatro hermanos” in the provinc...

  15. Simple Y-autosomal incompatibilities cause hybrid male sterility in reciprocal crosses between Drosophila virilis and D. americana.

    Science.gov (United States)

    Sweigart, Andrea L

    2010-03-01

    Postzygotic reproductive isolation evolves when hybrid incompatibilities accumulate between diverging populations. Here, I examine the genetic basis of hybrid male sterility between two species of Drosophila, Drosophila virilis and D. americana. From these analyses, I reach several conclusions. First, neither species carries any autosomal dominant hybrid male sterility alleles: reciprocal F(1) hybrid males are perfectly fertile. Second, later generation (backcross and F(2)) hybrid male sterility between D. virilis and D. americana is not polygenic. In fact, I identified only three genetically independent incompatibilities that cause hybrid male sterility. Remarkably, each of these incompatibilities involves the Y chromosome. In one direction of the cross, the D. americana Y is incompatible with recessive D. virilis alleles at loci on chromosomes 2 and 5. In the other direction, the D. virilis Y chromosome causes hybrid male sterility in combination with recessive D. americana alleles at a single QTL on chromosome 5. Finally, in contrast with findings from other Drosophila species pairs, the X chromosome has only a modest effect on hybrid male sterility between D. virilis and D. americana.

  16. Periods and dry environments in the seeds quality of Genipa americana L.Períodos e ambientes de secagem na qualidade de sementes de Genipa americana L.

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    Riselane de Lucena Alcântara Bruno

    2011-07-01

    Full Text Available Genipa americana L. is a tropical tree belonging to Rubiaceae family, providing fruit that are consumed in natura and is being increasingly explored as raw material to liquor, wine, soft drink and also the use of white wood for making various products. The seeds drying this species can be made using natural and or artificial methods. Thus, this study aimed to evaluate the effects of natural drying, in different periods and ambient, in the physiological quality of Genipa americana seeds. Seeds were submitted to the determination of moisture content after processing and drying of each period (0, 24, 48, 72, 96, 120, 144 and 168 hours. To evaluate the effects of ambient (laboratory and greenhouse and drying periods were performed the following tests: emergence in greenhouse, emergence speed index, seedling length and dry weight. Drying seed genipa can be done in a greenhouse (temperature of 33°C with relative humidity of 70% for 24 hours or laboratory environment (temperature of 28°C with relative humidity of 75% for 48 hours without compromising the physiological quality.Genipa americana L. é uma árvore tropical, pertencente à família Rubiaceae, fornece frutos que são consumidos in natura e vem sendo explorada cada vez mais como matéria-prima de licor, vinho, refresco e também o uso da madeira branca para confecção de vários produtos. A secagem de sementes dessa espécie pode ser feita pelo método natural e/ou artificial. Dessa forma, este trabalho teve por objetivo avaliar o efeito da secagem natural, em diferentes ambientes e períodos, na qualidade fisiológica das sementes de Genipa americana. As sementes foram submetidas à determinação do teor de água após o beneficiamento em cada período de secagem (0, 24, 48, 72, 96, 120, 144 e 168 horas. Para a avaliação do efeito dos ambientes (laboratório e ambiente telado e períodos de secagem foram realizados os seguintes testes: emergência em ambiente telado, índice de

  17. Cloning of a chitinase gene from Ewingella americana, a pathogen of the cultivated mushroom, Agaricus bisporus

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    P.W. Inglis

    2000-09-01

    Full Text Available We have isolated a gene encoding a chitinase (EC 3.2.1.14 from Ewingella americana, a recently described pathogen of the mushroom Agaricus bisporus. This gene, designated chiA (EMBL/Genbank/DDBJ accession number X90562, was cloned by expression screening of a plasmid-based E. americana HindIII genomic library in Escherichia coli using remazol brilliant violet-stained carboxymethylated chitin incorporated into selective medium. The chiA gene has a 918-bp ORF, terminated by a TAA codon, with a calculated polypeptide size of 33.2 kDa, likely corresponding to a previously purified and characterised 33-kDa endochitinase from E. americana. The deduced amino acid sequence shares 33% identity with chitinase II from Aeromonas sp. No. 10S-24 and 7.8% identity with a chitinase from Saccharopolyspora erythraeus. Homology to other chitinase sequences was otherwise low. The peptide sequence deduced from chiA lacks a typical N-terminal signal sequence and also lacks the chitin binding and type III fibronectin homology units common to many bacterial chitinases. The possibility that this chitinase is not primarily adapted for the environmental mineralisation of pre-formed chitin, but rather for the breakdown of nascent chitin, is discussed in the context of mushroom disease.O gene que codifica uma quitinase (EC 3.2.1.14 foi isolado de Ewingella americana, recentemente descrita como patógeno do cogumelo Agaricus bisporus. Este gene, denominado chiA (EMBL/Genebank/DDBJ número de acesso X9061, foi clonado e selecionado a partir de livraria genômica construída por digestão do DNA de E. americana com HindIII e ligação em plasmídio de expressão em E. coli, utilizando meio seletivo contendo quitina carboximetilada, corada com "remazol brilliant violet'' para seleção de clones. O gene chiA apresenta uma ORF de 918 bp, código terminador TAA, tendo o tamanho do polipeptídeo sido calculado como 33,2 kDa, o qual corresponde ao tamanho de 33 kDa da endoquitinase

  18. Macropropagation of Erythrina americana in a greenhouse: a potential tool for seasonally dry tropical forest restoration

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    Tara C. Fehling-Fraser

    2015-01-01

    Full Text Available L a deforestación en México ha hecho que la restauración sea una necesidad urgente. Erythrina americana es un árbol multipropósito, útil para la restauración de bosques tropicales estacionalmente secos (BTES, pero es poco estudiado. La macropropagación de esta especie es ventajosa comparada con la reproducción sexual que requiere de semillas escarificadas para germinar. En este estudio se evaluaron los efectos de la aplicación exógena de la fitohormona ácido indol-3-butírico en esquejes y del fertilizante fosfatado de liberación lenta (FLL en el sustrato, sobre el crecimiento de E. americana en invernadero. El experimento tuvo un diseño de cuatro bloques al azar. Diez esquejes se cosecharon por tratamiento después de 36, 66, 96 y 126 días de crecimiento. La sobrevivencia, biomasa seca total (BST, tasa de crecimiento relativo (TCR y la asignación de recursos (AR se evaluaron. La sobrevivencia promedio de los esquejes fue alta (95 %. Los tratamientos no afectaron la BST, TCR y AR; sin embargo, la aplicación de FLL en el sustrato mostró valores significativamente (P < 0.05 mayores de biomasa seca radical. Los periodos de crecimiento afectaron la TCR y AR. La macropropagación de E. americana fue exitosa y no requirió cuidados intensivos, por lo que esta técnica puede ser una alternativa viable para proyectos de restauración, principalmente en comunidades con bajos recursos.

  19. Technical approaches of a natural dye extracted from Phytolacca americana L.-berries with chemical mordants.

    Science.gov (United States)

    Park, Su-Youn; Jung, Suk-Yul

    2014-01-01

    Phytolacca americana L. is a large semi-succulent herbaceous plant which reaches three meters in height. It is native to eastern North America, the Midwest, and the Gulf Coast, with more scattered populations in the far West. It is imported into Korea and has been frequently used as a traditional natural drug for diseases such as systemic edema and nephritis. Its berries, that is, fruits are shiny dark purple held in racemous clusters on pink pedicels with a pink peduncle. They are round with a flat indented top and bottom. Immature berries are green, maturing into white and then blackish purple. It is not well known how the berries are used for a natural staining yet. In this study, using Phytolacca americana L.-berries, a natural staining was analyzed. Moreover, due to the broad use of chemical mordants, five different mordants including copper acetate, aluminum potassium sulfate, sodium tartrate plus citric acid, Iron II sulfate and potassium dichromate were combined. Extracted dye from the berries stained silk fabrics with ivory. The original purple color from the berries disappeared and transformed into ivory. Although the silk fabrics were differentially stained by the berries that were combined with mordants of aluminum potassium sulfate, sodium tartrate plus citric acid and potassium dichromate, only differences in lightness and darkness were observed. Interestingly, the combination of the dye from the berries with a mordant of copper acetate and Iron II sulfate induced the staining of the silk fabrics into khaki and dark khaki, respectively. This study is the first systemic report on staining silk fabrics with Phytolacca americana L.-berries and chemical mordants and suggests application of natural products to the fiber industry.

  20. Vascular riffle flora of Appalachian streams: the ecology and effects of acid mine drainage on Justificia americana (L. ) Vahl

    Energy Technology Data Exchange (ETDEWEB)

    Koryak, M.; Reilly, R.J.

    1984-06-01

    Justicia americana is a stout-based colonial plant, abundant in most of the larger, low to moderate gradient streams of the upper Ohio River basin. The distribution of J. americana is related to acid drainage from bituminous coal mining operations in the upper Ohio River drainage basin. Possible fluvial and biological consequences of the colonization or absence of Justicia are considered. Luxuriant growths were noted on gravel bars and riffles of larger, unpolluted streams in the basin. Acid mine drainage severely depresses the growth of the plant, leaving gravel shoals and riffles in the acid streams either barren or dominated by other emergent species. Particular among these new species is Elecocharis acicularis. The elimination of J. americana from suitable habitat adversely affects channel morphology, substrate composition, general aesthetic quality and aquatic stream life in the region. 16 references, 2 figures, 3 tables.

  1. Thermal effects on growth and respiration rates of the mayfly, Dolania americana (ephemeroptera)

    International Nuclear Information System (INIS)

    Harvey, R.S.

    1975-01-01

    The mayfly Dolania Americana, common in the sand of Upper Three Runs Creek, Savannah River Plant, was studied to determine the effects of seasonal changes in temperature on population growth rates and to determine the effects of slight elevations in water temperature on respiration rates of this benthic species. Growth of the population increased with stream temperature until peak emergence of adults in June and July. There was a strong inverse correlation between body weight and respiration rates of immature nymphs. Respiration rates at 2.5, 5, and 10 0 C above ambient creekwater temperatures were not significantly higher than those measured at ambient creekwater temperatures. (auth)

  2. Reflexões sobre as Independências Ibero-americanas

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    Manuel Chust

    2008-12-01

    Full Text Available O objetivo deste artigo é refletir acerca do tema das independências iberoamericanas do século XIX como objeto historiográfico, considerando-o em tempos e espaços amplos, gerais e de dimensões européias e americanas. Resgatando algumas das principais teses a respeito formuladas nas últimas quatro décadas, pretende-se examinar algumas posições em voga no momento, considerando-se a efervescência historiográfica anunciada pelas comemorações dos bicentenários no mundo ibérico.

  3. Effects of simulated acid rain on leaf anatomy and micromorphology of Genipa americana L. (Rubiaceae

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    Bruno Francisco Sant'Anna-Santos

    2006-03-01

    Full Text Available Experiments were conducted in order to characterize the injuries on leaf structure and micromorphology of G. americana and evaluate the degree of susceptibility of this species to simulated acid rain. Plants were exposed to acid rain (pH 3.0 for ten consecutive days. Control plants were submitted only to distilled water (pH 6.0. Leaf tissue was sampled and fixed for light and scanning electron microscopy. Necrotic interveinal spots on the leaf blade occurred. Epidermis and mesophyll cells collapse, hypertrophy of spongy parenchyma cells, accumulation of phenolic compounds and starch grains were observed in leaves exposed to acid rain. The micromorphological analysis showed, in necrotic areas, plasmolized guard cells and cuticle rupture. Epidermal and mesophyll cells alterations occurred before symptoms were visualized in the leaves. These results showed the importance of anatomical data for precocious diagnosis injury and to determine the sensitivity of G. americana to acid rain.Experimentos foram conduzidos para avaliar o grau de susceptibilidade e determinar as injúrias causadas pela chuva ácida simulada na anatomia e micromorfologia foliar de Genipa americana. Plantas foram expostas à chuva com pH 3,0 durante 10 dias consecutivos. No tratamento controle utilizou-se apenas água destilada (pH 6,0. Amostras foliares foram coletadas e fixadas para microscopia de luz e eletrônica de varredura. Foram observados nas folhas expostas à chuva ácida: necroses pontuais intervenais, colapso das células do mesofilo e da epiderme; hipertrofia do parênquima lacunoso e acúmulo de compostos fenólicos e grãos de amido. A análise micromorfológica evidenciou, nas áreas necrosadas, plasmólise das células-guarda e ruptura da cutícula e da crista estomática. Alterações anatômicas ocorreram antes que sintomas visuais fossem observados nas folhas. Estes resultados comprovam a importância de dados anatômicos na diagnose precoce da injúria e na

  4. Genipa americana: prospecção tecnológica

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    Selma Maria Santos Moura

    2016-10-01

    Full Text Available O jenipapo é uma árvore alta (10 a 15 metros, encontrada em regiões de clima tropical úmido, comum no nordeste brasileiro. Seu nome científico é Genipa americana L., jenipapo vem do Tupi-guarani jandipap, que significa fruto que serve para pintar. Apresenta caule reto, folhas verde-escuras, flores amarelo-ouro e o fruto na forma de baga ovoide, possui polpa marrom clara que envolve sementes no centro. Na alimentação humana, seu fruto é comestível ao natural e no preparo de doces, refrescos e vinho. É rico em ferro, vitaminas B1, B2, B5 e C, cálcio e hidratos de carbono. Na cultura popular têm indicações medicinais para o tratamento de afecções. Quando verde, o fruto fornece um suco azul muito utilizado como corante, transparente a princípio, o qual torna-se preto quando oxida, apresenta consistência do nanquim e no corpo, em contato com a pele, deixa manchas que desaparecem após uma semana ou mais, espontaneamente. O objetivo desta pesquisa foi realizar a prospecção tecnológica com relação à Genipa americana L., para apresentar uma visão geral sobre as tecnologias desenvolvidas relacionadas ao tema. Para isso realizou-se uma busca de patentes nas bases do European Patent Office, World Intellectual Property Organization, United States Patent and Trademark Office e no Instituto Nacional de Propriedade Industrial. Utilizando a palavra-chave Genipa americana L. foram registradas 88 patentes, quando acrescido à especificação corante encontrou-se 5 e nenhuma quando utilizou-se Genipa americana L. e célula. O Brasil registrou um número de patentes inferior ao encontrado nos USA e no Japão, sendo os anos de 2010, 2011 e 2015 os que apresentaram maior número. Ações integradas devem ser realizadas para estimular as instituições de fomento à pesquisa no intuito de viabilizar um ambiente propício à geração de inovações, fazendo do Brasil um país mais competitivo do ponto de vista tecnológico.  

  5. Dinámica de las concentraciones de carbono y nitrógeno en la descomposición de hojarascas de cultivos subtropicales del sureste de España

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    Carmen Rocío Rodríguez Pleguezuelo

    2017-12-01

    Full Text Available La descomposición de la hojarasca es una de las principales vías de consumo de energía en un ecosistema y uno de los pilares básicos en el ciclo de nutrientes. Por otro lado, en las últimas décadas, los cultivos subtropicales se han expandido de forma considerable en la costa de Granada (sureste de España. Para evaluar dichos ciclos se llevó a cabo un ensayo con bolsas utilizando para ello hojas de mango (Mangifera indica L., chirimoyo (Annona cherimola Mill., aguacate (Persea americana Mill. y níspero (Eriobotrya japonica L.. El objetivo de este trabajo fue realizar un seguimiento de la dinámica de descomposición de las hojarascas producidas por el mango, aguacate, chirimoyo y níspero, y de la evolución de su contenido en carbono y nitrógeno en condiciones de clima mediterráneo subtropical. Las bolsas fueron enterradas y se recuperaron cada cierto tiempo para evaluar la concentración de carbono y nitrógeno, así como la pérdida de masa en cada una de ellas. Los resultados de este estudio mostraron de forma evidente que los agricultores pueden beneficiarse del conocimiento de la dinámica de nutrientes en hojarasca para mejorar la materia orgánica en el suelo a largo plazo y la incorporación de nitrógeno. En este sentido, el níspero y el mango mostraron las mayores tasas de acumulación de nitrógeno y, por lo tanto, la biomasa de estas especies podría ser utilizada como enmiendas de tipo orgánico a largo plazo. Por el contrario, el chirimoyo acumuló mayores cantidades de carbono que el resto de los cultivos estudiados.

  6. Desarrollo floral del aguacate 'hass' en clima semicálido. Parte II. Generación y validación de modelos de predicción del desarrollo floral

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    S. Salazar-García

    2007-01-01

    Full Text Available Para disponer de herramientas que mejoren la productividad de los huertos de aguacate 'Hass' en el clima semicálido de Nayarit, México, se desarrolló esta investigación multianual (1998 a 2006 con los siguientes objetivos: a cuantificar el efecto de la temperatura ambiental sobre el desarrollo floral de 'Hass', y b desarrollar y validar modelos de predicción para pronosticar etapas críticas del proceso de floración. El desarrollo floral de 'Hass' fue asociado a la temperatura ambiental y pudo ser modelado matemáticamente. El desarrollo floral de brotes del flujo vegetativo de invierno estuvo asociado a la acumulación de días frío (DFA con temperaturas ¿ 21 °C, así como a los intervalos acumulados entre las temperaturas máxima y mínima (INTAC. Para los brotes del flujo de verano fueron los DFA con temperaturas ¿ 9 °C, ¿ 20 °C y los INTAC. Para brotes del flujo de invierno se obtuvieron dos modelos de predicción del desarrollo floral, inviernoDFA ¿ 21 (R2 = 0.99 e inviernoINTAC (R2 = 0.96. En el caso de los brotes del flujo de verano se generaron tres modelos de predicción del desarrollo floral, veranoDFA ¿ 19, veranoDFA ¿ 20 y veranoINTAC, todos ellos con R2 = 0.99.

  7. Effect of Agave americana and Agave salmiana Ripeness on Saponin Content from Aguamiel (Agave Sap).

    Science.gov (United States)

    Leal-Díaz, Ana María; Santos-Zea, Liliana; Martínez-Escobedo, Hilda Cecilia; Guajardo-Flores, Daniel; Gutiérrez-Uribe, Janet Alejandra; Serna-Saldivar, Sergio Othón

    2015-04-22

    Steroidal saponins have shown beneficial health effects. Agave spp. leaves and rhizomes are sources of these compounds, but their presence has not been reported in the aguamiel. Aguamiel is the sweet edible sap from mature agave, and its quality is influenced by the plant ripening stage. The purpose of this research was to identify and quantitate saponins in aguamiel from Agave americana and Agave salmiana at two ripening stages. Saponins and sapogenins were identified with HPLC/ESI-MS/TOF and quantitated with HPLC/ELSD. Results proved the presence of saponins derived from kammogenin, manogenin, gentrogenin, and hecogenin. The saponin content in aguamiel from immature A. salmiana was 2-fold higher (478.3 protodioscin equivalents (PE) μg/g aguamiel (DM)) compared with A. americana (179.0 PE μg/g aguamiel (DM)). In both species, saponin content decreased when plants reached sexual maturity. This should be considered before evaluating the effects of Agave spp. as a source of bioactive saponins.

  8. Evaluation of in vitro antioxidant and anti-inflmmatory activities of Ximenia americana extracts

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    Arun Kashivishwanath Shettar

    2015-11-01

    Full Text Available Objective: To evaluate in vitro antioxidant and anti-inflammatory activity of Ximenia americana extracts. Methods: Herbal extraction was done by Soxhlet extraction method with increasing polarity of solvents viz., chloroform, ethyl acetate, methanol, ethanol and water. Phytochemical analysis was done using different biochemical tests. Antioxidant potential of plant extracts were analyzed by ferric ion reducing antioxidant power, phosphomolybdenum and 2,2-diphenyl-1- picrylhydrazyl, and anti-inflammatory activity by using protein denaturation in vitro bioassay. Total phenolic content of each extract was also determined to assess their corresponding effect on antioxidant capacity of plant. Results: Phytochemical analysis showed that each solvent extract contained broad spectrum of secondary metabolites, phenolic compounds, flavonoids, tannins and glycosides, whereas compared to other solvent extracts, chloroform extract showed negative result for phenolic compounds whereas aqueous extract exhibited the highest phenolic content and the significant antioxidant capacity based on the test performed. Out of all extracts, methanol extract showed high anti-inflammatory activity. Conclusions: The present study revealed that different solvent extracts of Ximenia americana leaves contain broad spectrum of bioactive compounds. Results confirm that aqueous extract exhibited high antioxidant activity and methanol extract exhibited high antiinflammatory activity. Further study requires purification, characterization and structural elucidation of phenolic compounds in both extracts that may help in the development of new phytopharmaceuticals.

  9. Transplantation immunity in the American cockroach, Periplaneta americana: the rejection of integumentary grafts from Blatta orientalis.

    Science.gov (United States)

    Karp, R D; Meade, C C

    1993-01-01

    The results from several previous studies have indicated that the American cockroach is able to respond to integumentary xenografts, but doubt remained as to whether cockroaches could effectively discriminate between self and allogeneic differences. This was emphasized by the fact that while one study reported that Periplaneta americana responded to grafts donated by the closely related genus Blatta orientalis, the data reported elsewhere, using a different assay system, found no such reactivity. Since we have subsequently reported, using a direct histological assay, that Periplaneta can in fact recognize and destroy integumentary allografts, we initiated a study to hopefully sort out the enigma presented by previous data using Blatta as a transplant donor. Integument from Blatta orientalis was transplanted orthotopically onto Periplaneta americana, and at various time points post-transplant, scored histologically for the survival of the subcuticular epidermal layer. The results clearly demonstrated that Periplaneta reacted to the Blatta tissue, because approximately 82% of the grafts had the epidermal layer destroyed by day 7 posttransplant. The kinetics of the response to Blatta was more in line with our allograft data, which would be in agreement with other work indicating that the closer the donor and recipient are on the phylogenetic tree, the less intense the reactivity to the foreign transplant.

  10. Characterization of an Invertebrate-Type Dopamine Receptor of the American Cockroach, Periplaneta americana

    Directory of Open Access Journals (Sweden)

    Britta Troppmann

    2014-01-01

    Full Text Available We have isolated a cDNA coding for a putative invertebrate-type dopamine receptor (Peadop2 from P. americana brain by using a PCR-based strategy. The mRNA is present in samples from brain and salivary glands. We analyzed the distribution of the PeaDOP2 receptor protein with specific affinity-purified polyclonal antibodies. On Western blots, PeaDOP2 was detected in protein samples from brain, subesophageal ganglion, thoracic ganglia, and salivary glands. In immunocytochemical experiments, we detected PeaDOP2 in neurons with their somata being located at the anterior edge of the medulla bilaterally innervating the optic lobes and projecting to the ventro-lateral protocerebrum. In order to determine the functional and pharmacological properties of the cloned receptor, we generated a cell line constitutively expressing PeaDOP2. Activation of PeaDOP2-expressing cells with dopamine induced an increase in intracellular cAMP. In contrast, a C-terminally truncated splice variant of this receptor did not exhibit any functional property by itself. The molecular and pharmacological characterization of the first dopamine receptor from P. americana provides the basis for forthcoming studies focusing on the significance of the dopaminergic system in cockroach behavior and physiology.

  11. Characterization of an invertebrate-type dopamine receptor of the American cockroach, Periplaneta americana.

    Science.gov (United States)

    Troppmann, Britta; Balfanz, Sabine; Krach, Christian; Baumann, Arnd; Blenau, Wolfgang

    2014-01-06

    We have isolated a cDNA coding for a putative invertebrate-type dopamine receptor (Peadop2) from P. americana brain by using a PCR-based strategy. The mRNA is present in samples from brain and salivary glands. We analyzed the distribution of the PeaDOP2 receptor protein with specific affinity-purified polyclonal antibodies. On Western blots, PeaDOP2 was detected in protein samples from brain, subesophageal ganglion, thoracic ganglia, and salivary glands. In immunocytochemical experiments, we detected PeaDOP2 in neurons with their somata being located at the anterior edge of the medulla bilaterally innervating the optic lobes and projecting to the ventro-lateral protocerebrum. In order to determine the functional and pharmacological properties of the cloned receptor, we generated a cell line constitutively expressing PeaDOP2. Activation of PeaDOP2-expressing cells with dopamine induced an increase in intracellular cAMP. In contrast, a C-terminally truncated splice variant of this receptor did not exhibit any functional property by itself. The molecular and pharmacological characterization of the first dopamine receptor from P. americana provides the basis for forthcoming studies focusing on the significance of the dopaminergic system in cockroach behavior and physiology.

  12. ECOPHYSIOLOGICAL ANALYSIS OF GENIPA AMERICANA L. SEEDS IN AN INDUCED SOIL SEED BANK

    Directory of Open Access Journals (Sweden)

    Francesca Salla

    2016-03-01

    Full Text Available This work studied the behavior of G. americana L. (Rubiaceae seeds in an induced soil seed bank. This study was conducted in Lavras-MG in a seasonal semideciduous forest of the Federal University of Lavras. Seeds collected in the area were cleaned and analyzed (water content, viability and desiccation tolerance. Soil banks were installed in two distinct areas (top of hill and riparian forest located in the understory of the forest. Seeds were placed in nylon nets and covered with a thin litter layer that was previously removed for this purpose. Monthly assessment of germination, seedling emergence, seed and soil water content, and the characterization of the initial seedling growth was performed. It was observed that G. americana seeds forms a transitory seed bank, with maintenance of its viability until the fourth month after disposal in natural environment. In general, the seed bank located in the riparian forest area showed the best potential for seedling establishment in a natural environment, which supports the ecological behavior of this species.

  13. Simulating the effects of climate change on population connectivity of American marten (Martes americana) in the northern Rocky Mountains, USA

    Science.gov (United States)

    T. N. Wasserman; S. A. Cushman; A. S. Shirk; E. L. Landguth; J. S. Littell

    2012-01-01

    We utilize empirically derived estimates of landscape resistance to assess current landscape connectivity of American marten (Martes americana) in the northern Rocky Mountains, USA, and project how a warming climate may affect landscape resistance and population connectivity in the future. We evaluate the influences of five potential future temperature scenarios...

  14. Use of culture filtrates of Ceratocystis ulmi as a bioassay to screen for disease tolerant Ulmus americana

    Science.gov (United States)

    Paula M. Pijut; Subash C. Domir; R. Daniel Lineberger; Lawrence R. Schreiber

    1990-01-01

    Callus cultures of elm (Ulmus americana L.) derived from Dutch elm disease susceptible, intermediate-resistant, and resistant genotypes were exposed to the culture filtrates of three pathogenic isolates of Ceratocystis ulmi, the causal agent of Dutch elm disease. Callus fresh weights, cell viability, and reactions of stem cuttings...

  15. Seed rescue from photoperiod sensitive American Joint Vetch (Aeschynomene americana L.) accessions using hydroponic cloning and aeroponics

    Science.gov (United States)

    American joint vetch, Aeschynomene Americana L. is a self-pollinated tropical pasture legume and the USDA, ARS, PGRCU curates 137 accessions from the United States, S. America, Mexico, Central America, and Zambia. Many accessions in this collection are photoperiod sensitive due to their typical flow...

  16. The Core Gut Microbiome of the American Cockroach, Periplaneta americana, Is Stable and Resilient to Dietary Shifts.

    Science.gov (United States)

    Tinker, Kara A; Ottesen, Elizabeth A

    2016-11-15

    The omnivorous cockroach Periplaneta americana hosts a diverse hindgut microbiota encompassing hundreds of microbial species. In this study, we used 16S rRNA gene sequencing to examine the effect of diet on the composition of the P. americana hindgut microbial community. Results show that the hindgut microbiota of P. americana exhibit a highly stable core microbial community with low variance in compositions between individuals and minimal community change in response to dietary shifts. This core hindgut microbiome is shared between laboratory-hosted and wild-caught individuals, although wild-caught specimens exhibited a higher diversity of low-abundance microbes that were lost following extended cultivation under laboratory conditions. This taxonomic stability strongly contrasts with observations of the gut microbiota of mammals, which have been shown to be highly responsive to dietary change. A comparison of P. americana hindgut samples with human fecal samples indicated that the cockroach hindgut community exhibited higher alpha diversity but a substantially lower beta diversity than the human gut microbiome. This suggests that cockroaches have evolved unique mechanisms for establishing and maintaining a diverse and stable core microbiome. The gut microbiome plays an important role in the overall health of its host. A healthy gut microbiota typically assists with defense against pathogens and the digestion and absorption of nutrients from food, while dysbiosis of the gut microbiota has been associated with reduced health. In this study, we examined the composition and stability of the gut microbiota from the omnivorous cockroach Periplaneta americana. We found that P. americana hosts a diverse core gut microbiome that remains stable after drastic long-term changes in diet. While other insects, notably ant and bee species, have evolved mechanisms for maintaining a stable association with specific gut microbiota, these insects typically host low-diversity gut

  17. Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections.

    Science.gov (United States)

    Gómez-Calderón, Cecilia; Mesa-Castro, Carol; Robledo, Sara; Gómez, Sergio; Bolivar-Avila, Santiago; Diaz-Castillo, Fredyc; Martínez-Gutierrez, Marlen

    2017-01-18

    The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. The objective of this study was to identify some of the compounds that are present in Mammea americana (M. americana) and Tabernaemontana cymosa (T. cymosa) plants and, subsequently, to evaluate their cytotoxicity in VERO cells and their potential antiviral effects on DENV and CHIKV infections in those same cells. Dry ethanolic extracts of M. americana and T. cymosa seeds were subjected to open column chromatographic fractionation, leading to the identification of four compounds: two coumarins, derived from M. americana; and lupeol acetate and voacangine derived from T. cymosa.. The cytotoxicity of each compound was subsequently assessed by the MTT method (at concentrations from 400 to 6.25 μg/mL). Pre- and post-treatment antiviral assays were performed at non-toxic concentrations; the resulting DENV inhibition was evaluated by Real-Time PCR, and the CHIKV inhibition was tested by the plating method. The results were analyzed by means of statistical analysis. The compounds showed low toxicity at concentrations ≤ 200 μg/mL. The compounds coumarin A and coumarin B, which are derived from the M. americana plant, significantly inhibited infection with both viruses during the implementation of the two experimental strategies employed here (post-treatment with inhibition percentages greater than 50%, p treatment with percentages of inhibition greater than 40%, p treatment strategy (at inhibition percentages greater than 70%, p treating Dengue and Chikungunya fever. Additionally, lupeol acetate and voacangine efficiently inhibit infection with DENV, also turning them into promising antivirals for Dengue fever.

  18. Reunión y escritura femeninas en los contextos de las independencias americanas

    Directory of Open Access Journals (Sweden)

    Patricia Martínez i Álvarez

    2010-10-01

    Full Text Available En los ámbitos de las relaciones de género y la representación de la diferencia sexual femenina, las mujeres –en el contexto de los procesos de emancipación y de construcción de los nuevos Estados en América Latina en el siglo XIX– llevaron a cabo proyectos propios de significación, al margen de los proyectos políticos que había en el escenario. El objetivo de la investigación que sustenta el artículo, es dar lectura, a la luz de los sentidos que las mujeres les dieron, a textos y contextos de aquel momento. Se recurre a fragmentos de dos de las obras literarias más importantes de finales del siglo XIX, para afirmar una historia de las mujeres que invita al final de la colonialidad americana.

  19. Reunión y escritura femeninas en los contextos de las independencias americanas

    Directory of Open Access Journals (Sweden)

    Patricia Martínez i Álvarez

    2010-01-01

    Full Text Available En los ámbitos de las relaciones de género y la representación de la diferencia sexual femenina, las mujeres en el contexto de los procesos de emancipación y de construcción de los nuevos Estados en América Latina en el siglo XIX llevaron a cabo proyectos propios de significación, al margen de los proyectos políticos que había en el escenario. El objetivo de la investigación que sustenta el artículo, es dar lectura, a la luz de los sentidos que las mujeres les dieron, a textos y contextos de aquel momento. Se recurre a fragmentos de dos de las obras literarias más importantes de finales del siglo XIX, para afirmar una historia de las mujeres que invita al final de la colonialidad americana.

  20. Cloning and characterization of the adipokinetic hormone receptor from the cockroach Periplaneta americana

    DEFF Research Database (Denmark)

    Hansen, Karina K; Hauser, Frank; Cazzamali, Giuseppe

    2006-01-01

    Cockroaches have long been used as insect models to investigate the actions of biologically active neuropeptides. Here, we describe the cloning and functional expression in Chinese hamster ovary cells of an adipokinetic hormone (AKH) G protein-coupled receptor from the cockroach Periplaneta...... americana. This receptor is only activated by various insect AKHs (we tested eight) and not by a library of 29 other insect or invertebrate neuropeptides and nine biogenic amines. Periplaneta has two intrinsic AKHs, Pea-AKH-1, and Pea-AKH-2. The Periplaneta AKH receptor is activated by low concentrations...... of both Pea-AKH-1 (EC50, 5 x 10(-9)M), and Pea-AKH-2 (EC50, 2 x 10(-9)M). Insects can be subdivided into two evolutionary lineages, holometabola (insects with a complete metamorphosis during development) and hemimetabola (incomplete metamorphosis). This paper describes the first AKH receptor from...

  1. FORMULASI NANOPARTIKEL EKSTRAK BAWANG DAYAK (Eleutherine americana (Aubl Merr DENGAN VARIASI KONSENTRASI KITOSAN-TRIPOLIFOSFAT (TPP

    Directory of Open Access Journals (Sweden)

    Ermina Pakki

    2016-12-01

    Full Text Available Ekstrak etanol Bawang dayak (Eleutherine americana (Aubl Merr. diketahui memiliki aktivitas antioksidan yang kuat sehingga berpotensi untuk dikembangkan menjadi sistem penghantaran nanopartikel. Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi kitosan – tripolifosfat (TPP terhadap karakteristik fisik dari nanopartikel. Ekstrak bawang dayak diformulasi dalam bentuk nanopartikel dengan metode gelasi ionik dengan variasi konsentrasi polimer kitosan : tripolifosfat yaitu 0,5% : 0,5% (F1, 0,75% : 0,5% (F2, dan 1% : 0,5% (F3. Parameter pengujian meliputi penentuan ukuran dan indeks polidispersitas nanopartikel menggunakan particle size analyzer, pengamatan morfologi menggunakan scanning electron microscopy, pengukuran efisiensi penjerapan, dan disolusi in vitro. Hasil penelitian menunjukkan bahwa nanopartikel F1, F2, dan F3 memiliki ukuran masing-masing sebesar 256,30 nm, 376,28 nm dan 419,18 nm dengan distribusi ukuran yang relatif homogen dan efisiensi penjerapan masing-masing sebesar 69,54%, 77,51% dan 79,79%. Pengamatan morfologi dari nanopartikel menunjukkan bentuk partikel yang mendekati spheris (bulat dengan permukaan yang kasar. Profil pelepasan obat dari nanopartikel F1, F2, dan F3 pada jam ke-8 masing-masing sebesar 71,19 % (F1, 74,97% (F2 dan 80,55% (F3. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa ekstrak bawang dayak dapat diformulasi dalam ukuran nanopartikel dengan karakteristik fisik yang bervariasi tergantung pada konsentrasi kitosan dan tripolifosfat yang digunakan. Kata Kunci:   Bawang dayak (Eleutherine americana (Aubl Merr., antioksidan, nanopartikel, kitosan, tripolifosfat, gelasi ionik. ABSTRACT Bawang dayak (Eleutherine americana (Aubl Merr. ethanol extract had been known to have excellent antioxidant activity that has the potential to be developed into a nanoparticle delivery systems. This study aims to determine the effect of varying concentrations of chitosan

  2. A Revista Ibero-Americana De Estratégia Em 2016 E 2017

    Directory of Open Access Journals (Sweden)

    Fernando Antonio Ribeiro Serra

    2018-04-01

    Full Text Available Nestes últimos dois anos a Revista Ibero-Americana de Estratégia (RIAE – Iberoamerican Journal of Strategic Management (IJSM tem procurado aprimorar e consolidar as mudanças realizadas desde 2014. Este movimento não poderia acontecer sem que os autores, a quem agradecemos, apesar das diversas alternativas de periódicos onde submeter os seus trabalhos, tenham escolhido a RIAE/IJSM. Também não seria possível sem o esforço e dedicação dos revisores. Agradecemos aos nossos revisores, não só pela dedicação, mas pelo esforço que têm feito em melhorar a qualidade das revisões e apontar possibilidades de melhoria, mesmo para os artigos que por ventura não sejam aceitos.

  3. Diversity and genetic structure of jenipapo (Genipa americana L. Brazilian accessions

    Directory of Open Access Journals (Sweden)

    Ana Veruska Cruz da Silva

    2014-10-01

    Full Text Available Usually known as jenipapo, Genipa americana L. is a Rubiaceae Brazilian native species. It is an important species in the restoration of Brazilian riparian forests and is one of the most promising fruit trees for sustainable harvesting programs. In this study we provide the first data on the genetic diversity and structure of a Jenipapo Germplasm Bank using inter simple sequence repeat (ISSR markers. We evaluated 160 accessions from wild populations, and the data generated from 12 ISSR primers were used to determine genetic variability via a model-based Bayesian procedure (Structure and molecular variance analysis. In addition, Shannon index, genetic diversity and Jaccard coefficients were estimated. A total of 12 primers were used, which generated 123 polymorphic fragments. Four groups were formed from the analysis of the fragments, and the CR1-2 genotype was isolated, being more divergent than the other genotypes.

  4. La fruticultura subtropical en los ecosistemas de ladera de clima mediterráneo: cambios en la vegetación/Subtropical fruit farming in Mediterranean weather hillside ecosystem: changes in vegetation

    Directory of Open Access Journals (Sweden)

    Carmen Rocío Rodríguez-Pleguezuelo

    2017-09-01

    Full Text Available En la costa de Granada, sureste de España, se encuentra una zona de frutales subtropicales en laderas, distribuidos en terrazas. Se evaluaron tasas de descomposición y liberación de nitrógeno (N de hojarasca, en dos ecosistemas: (1 ecosistema de ladera inalterado (ELI sin cultivo agrícola, con cubierta de vegetación autóctona, y (2 ecosistema de ladera alterado (ELA con frutales de mango (Mangifera indica L., aguacate (Persea americana Mill., chirimoyo (Annona cherimolia Mill., níspero (Eriobotrya japonica (Thunb. Lindl., y cubiertas de plantas aromáticas y medicinales (PAM en los taludes de las terrazas. En ELA la tasa de descomposición de hojarasca de chirimoyo fue la más alta, y entre las PAM la alhucema rizada (Lavandula dentata L., así como la mejorana (Thymus mastichina L., alhucema rizada (Lavandula dentata L. y ajedrea (Satureja obovata Lag. registraron altas tasas de liberación de N. En ELI acebuche (Olea europea L. y bolina (Genista umbellata (L'Hér. Dum., tuvieron bajas tasas de descomposición. Las PAM en ELA pueden contribuir a restablecer los ciclos naturales de reciclado de nutrientes, minimizando los efectos del cambio de uso del suelo.

  5. PLANTAS ÚTILES EN LOS SISTEMAS AGROFORESTALES TRADICIONALES DEL LITORAL ECUATORIANO

    Directory of Open Access Journals (Sweden)

    Luz García Cruzatty

    2008-12-01

    Full Text Available El estudio se realizó en la Cuenca Media del Río Guayas, Ecuador, en el área correspondiente a los cantones Mocache y Palenque. El inventario de las especies se obtuvo mediante recorridos participativos. Las especies se clasificaron de acuerdo al Sistema Filogenético de Cronquist. Los datos sobre el uso de las especies se obtuvieron mediante charlas y entrevistas no formales. Se determinó que los agricultores de la Cuenca Media del Río Guayas conocen y utilizan 262 especies vegetales que pertenecen a 67 familias y 164 géneros. Las familias vegetales más comunes fueron: Anacardiaceae, Papilonaceae, Solanaceae, Rutaceae, Musaceae y Lamiaceae, con más de diez especies cada una. Se documentaron 21 tipos de usos, de los cuales los más importantes son: alimenticio, medicinal y construcción, lo que demuestra la preocupación de las familias por satisfacer sus necesidades básicas. La mayoría de las especies utilizadas son árboles, seguido por hierbas y arbustos. Entre las especies de mayor importancia por los múltiples usos y productos que se obtienen están: Persea americana Mill. (aguacate, Artocarpus altilis Fosberg (fruta de pan, Musa sp. (plátano, Manguifera sp. (mango, Phythelephas aequatoriales Spruce (cade, Inga edulis Matius (guaba de bejuco, entre otros.

  6. Morfologia das células do sangue periférico em emas (Rhea americana

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    Eunice Anita de Moura Fortes

    2009-06-01

    Full Text Available The rhea (Rhea americana is a South American bird of the ratite group and of the Rheiformes order. It has been exploited for economical purposes, as cattle alternative in European and South American countries. In Brazil, the State of Rio Grande do Sul is outstanding, in rhea rearing and it is in the process of implantation in the Country Northeasty Region. This work aims to describe the morphology of the blood cells in rheas. In this work ten rheas were used, regardless age and sex. Two ml of peripheral blood were collected by puncture of the brachial vein with disposable syringe. The samples here partially used to make extensions with Leishman stain. Seven types of nucleate cells have been observed through morphologic analysis on the light microscope. The erythrocyte revealed an elliptical form, with condensed nucleus of elliptical form; acidophilic cytoplasm. The thrombocyte revealed an elliptical form, with nucleus located in one of the polar regions; pale cytoplasm. As to the round-shaped leukocytes, within the granulocytes, the heterophils presented excentric, condensed, and lobulated nucleus; cytoplasm rich in fusiform salmon-colored granules. The eosinophils distinguish from the heterophils due to the round eosinophilic granules. The basophils stand out from the other granulocytes due to its large and central nucleus with round specific cytoplasmic and highly basophilic granules. Within the agranulocytes, the monocytes presented reniform nucleus, which is frequently central, with slack chromatin, with small areas of condensation; cytoplasm lightly basophilic and with vacuoles. The lymphocytes presented varies forms and sizes; large nucleus with slack chromatin with some nucleoli; scarce and basophilic cytoplasm. The cells of the peripheral blood of Rhea americana present on the light microscope morphology similar to the other birds which have already been studied.

  7. Monitoramento de fitoplâncton e microcistina no reservatório da UHE Americana Monitoring phytoplankton and microcistyn at the Americana reservoir

    Directory of Open Access Journals (Sweden)

    R.A.R. Ferreira

    2005-06-01

    Full Text Available Este trabalho foi realizado na UHE Americana, pertencente à Companhia Paulista de Força e Luz, e faz parte de um projeto de pesquisa e desenvolvimento realizado em conjunto com a Faculdade de Ciências Agronômicas - UNESP, de Botucatu. As amostragens de água foram realizadas nos meses de fevereiro, abril, junho e outubro de 2004. As características analisadas foram: temperatura da água, pH, oxigênio dissolvido, condutividade, nitrogênio total, nitrito, nitrato, amônia, fósforo total, fosfato, fosfato inorgânico, juntamente com análise qualitativa e quantitativa da comunidade fitoplanctônica e a toxicidade. O reservatório apresentou valores elevados de fósforo total, variando de 18 a 509 µg L-1; fosfato, de 4 a 463 µg L-1; nitrogênio total, de 0,99 a 17,25 mg L-1; e nitrato, de 0,26 a 15,29 mg L-1. Para a comunidade fitoplanctônica foram encontrados 103 táxons em todo o período amostrado; a maior riqueza foi encontrada no ponto P06, e a maior pobreza de táxons, nos pontos localizadas no corpo central do reservatório (P02, P03, P04 e P05. A maior concentração de cianofícea ocorreu em abril de 2004: 5.375.175 ind. L-1. As espécies que apresentaram as maiores densidades foram Microcystis aeruginosa, Anabaena spiroides, Microcystis sp. e Pseudoanabaena mucicola; a maior densidade foi apresentada por Anabaena spiroides, com 4.178.084 ind. L-1. Nos meses de junho e outubro a classe Cryptophyceae teve uma grande contribuição para a densidade total. Apesar da grande densidade de cianobactérias, os valores de toxicidade ficaram abaixo do limite permitido pela Portaria nº 1.469.This work was carried out at the Americana Reservoir, owned by Companhia Paulista de Força e Luz, and was part of a joint R&D project with Faculdade de Ciências Agronômicas - Botucatu - São Paulo - Brazil. Water sampling was collected in February, April, June and October 2004. The following characteristics were analyzed: water temperature, p

  8. Thomas Jefferson, I dilemmi della democrazia americana, translated and edited by Alberto Giordano, with a preface by Dino Cofrancesco (Novi Ligure: Città del Silenzio, 2007

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    Pierluigi Chiassoni

    2010-03-01

    Full Text Available A review of Thomas Jefferson, I dilemmi della democrazia americana, translated and edited by Alberto Giordano, with a preface by Dino Cofrancesco (Novi Ligure: Città del Silenzio, 2007

  9. Proconsuls and CINCs from the Roman Republic to the Republic of the United States of America: Lessons for the Pax Americana

    National Research Council Canada - National Science Library

    Bradford, Jeffrey

    2001-01-01

    Political and media pundits have labeled the current period of post Cold-War world order the Pax Americana, reminiscent of the Pax Romana that occurred from 27 to 180 AD, during the zenith of the Roman Empire...

  10. LOS MACROINVERTEBRADOS COMO INDICADORES DE LA CALIDAD DEL SUELO EN CULTIVOS DE MORA, PASTO Y AGUACATE THE MACROINVERTEBRATES AS INDICATORS OF THE QUALITY OF SOIL IN BLACKBERRY, GRASS AND AVOCADO CROPS

    Directory of Open Access Journals (Sweden)

    Santiago Rendón Pareja

    2011-06-01

    Full Text Available El estado de las propiedades dinámicas del suelo, tales como el contenido de la materia orgánica, la diversidad de organismos, o los productos microbianos en un tiempo particular, permiten inferir sobre la calidad del suelo. Los indicadores disponibles para evaluarla, pueden variar entre localidades, dependiendo del tipo y uso del suelo, función y factores de formación del mismo. Los invertebrados se pueden constituir en indicadores de la calidad de un suelo, dado que juegan un papel vital en los procesos de ciclaje de nutrientes; además, su diversidad, número y funciones son sensibles al cambio ambiental en las condiciones del suelo, asociadas con actividades propias en los agroecosistemas. Con el propósito de cualificar la calidad del suelo en varios sistemas productivos, se evaluó la presencia de macroinvertebrados en cultivos de mora, pasto y aguacate, empleando para el muestreo la técnica del monolito propuesto por Instituto de Fertilidad y Biología de Suelos Tropicales (TSBF y luego se procedió a identificarlos a nivel de familia. La mayor cantidad de macroinvertebrados se encontró en los primeros 10 cm, siendo el cultivo de mora el que registró la mayor diversidad.The state of soil dynamic properties, such as organic matter content, diversity of organisms, or microbial products in a particular time, allow to infer about soil quality. The indicators available to evaluate, may vary among locations, depending on the type and land use, function and factors of formation. Invertebrates can be indicators of soil quality, as they play a vital role in nutrient cycling processes, furthermore, their diversity, number and functions are sensitive to environmental change in soil conditions associated with activities own in agroecosystems. In order to qualify the quality of soil in various production systems was evaluated the macroinvertebrates presence in mulberry, pasture and avocado crops, using the monolith sampling technique proposed

  11. INFLUENCIA DEL CLIMA, HUMEDAD DEL SUELO Y ÉPOCA DE FLORACIÓN SOBRE LA BIOMASA Y COMPOSICIÓN NUTRIMENTAL DE FRUTOS DE AGUACATE "HASS" EN MICHOACÁN, MÉXICO

    Directory of Open Access Journals (Sweden)

    Samuel Salazar-García

    2011-01-01

    Full Text Available El amplio periodo de cosecha del aguacate "Hass" en Michoacán se debe a la diversidad de condiciones de cultivo y a la ocurrencia de múltiples flujos vegetativos que resultan en varios flujos de floración y épocas de cosecha. El objetivo de este estudio fue evaluar la influencia de tres condiciones climáticas (semicálido subhúmedo, semicálido húmedo y templado subhúmedo, el régimen de humedad del suelo (con y sin riego y la época de floración ("loca": agosto-septiembre, "normal": diciembre-febrero, y "marceña": febrero-marzo, sobre la biomasa de diferentes partes del fruto y su composición nutrimental. Se seleccionaron seis árboles en cada uno de seis huertos comerciales y de cada árbol se tomaron 10 frutos (¿ 21.5 % materia seca de la pulpa por época de floración. Los frutos fueron seprados en epidermis, pulpa, testa y embrión. A cada parte se le determinó peso fresco (PF, peso seco (PS, materia seca (MS y composición nutrimental. A diferencia del clima y régimen de humedad del suelo, la época de floración afectó el PF, PS y MS de las partes del fruto,así como su composición nutrimental, especialmente K, Ca, Mg, S, Fe, Cu, Mn, Zn y B. En el clima templado subhúmedo se tuvieron los mayores contenidos de N y Mn en epidermis; N, P, K, Ca y Mg en pulpa; Mg, S, Cu y Mn en testa, y B en testa y embrión. Los huertos sin riego mostraron concentraciones más altas de Ca, S y Cu en epidermis; P, K, Mn, Ca y Cu en pulpa; K, Mn y Cu en testa, y de P, K, Mn en embrión

  12. Antibacterial effect of crude extract and metabolites of Phytolacca americana on pathogens responsible for periodontal inflammatory diseases and dental caries.

    Science.gov (United States)

    Patra, Jayanta Kumar; Kim, Eun Sil; Oh, Kyounghee; Kim, Hyeon-Jeong; Kim, Yangseon; Baek, Kwang-Hyun

    2014-09-20

    The oral cavity is the store house of different species of microorganisms that are continuously engaged in causing diseases in the mouth. The present study was conducted to evaluate the antibacterial potential of crude extracts of the aerial parts of Phytolacca americana and its natural compounds against two oral pathogens, Porphyromonas gingivalis and Streptococcus mutans, which are primarily responsible for periodontal inflammatory diseases and dental caries, as well as a nonpathogenic Escherichia coli. Crude extract and fractions from the aerial parts of P. americana (0.008-1.8 mg/mL) were evaluated for their potential antibacterial activity against two oral disease causing microorganisms by micro-assays. The standard natural compounds present in P. americana, kaempferol, quercetin, quercetin 3-glucoside, isoqueritrin and ferulic acid, were also tested for their antibacterial activity against the pathogens at 1-8 μg/mL. The crude extract was highly active against P. gingivalis (100% growth inhibition) and moderately active against S. mutans (44% growth inhibition) at 1.8 mg/mL. The chloroform and hexane fraction controlled the growth of P. gingivalis with 91% and 92% growth inhibition at a concentration of 0.2 mg/mL, respectively. Kaempferol exerted antibacterial activity against both the pathogens, whereas quercetin showed potent growth inhibition activity against only S. mutans in a concentration dependent manner. The crude extract, chloroform fraction, and hexane fraction of P. americana possesses active natural compounds that can inhibit the growth of oral disease causing bacteria. Thus, these extracts have the potential for use in the preparation of toothpaste and other drugs related to various oral diseases.

  13. Simple Y-Autosomal Incompatibilities Cause Hybrid Male Sterility in Reciprocal Crosses Between Drosophila virilis and D. americana

    OpenAIRE

    Sweigart, Andrea L.

    2010-01-01

    Postzygotic reproductive isolation evolves when hybrid incompatibilities accumulate between diverging populations. Here, I examine the genetic basis of hybrid male sterility between two species of Drosophila, Drosophila virilis and D. americana. From these analyses, I reach several conclusions. First, neither species carries any autosomal dominant hybrid male sterility alleles: reciprocal F1 hybrid males are perfectly fertile. Second, later generation (backcross and F2) hybrid male sterility ...

  14. Survival and weight change among adult individuals of Periplaneta americana (Linnaeus, 1758 (Blattaria, Blattidae subject to various stress conditions

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    Jucelio Peter Duarte

    2015-05-01

    Full Text Available Periplaneta americana is a species of great importance to public health, since it can act as a vector of many pathogens and it reaches large populations in urban environments. This is probably due to its ability to resist starvation and desiccation. This study aimed to evaluate the effects of absence of water and food on survival and weight change among adult P. americana individuals and check whether the initial weight of individuals influences on their survival. Four groups having twenty P. americana couples were formed and subject to: I no water or food; II no food; III no water; and IV control group. Insects were isolated according to the groups, which were weighed at the beginning and end of the stress conditions. They remained under these conditions until all individuals in each test group were dead. Stress conditions caused reduction in survival time when compared to the control group. Adults with higher body mass survived longer when deprived only of food, while among those lacking water, weight had no influence on survival. Total weight loss was greater among individuals deprived of water than those deprived only of food.

  15. Survival and weight change among adult individuals of Periplaneta americana (Linnaeus, 1758 (Blattaria, Blattidae subject to various stress conditions

    Directory of Open Access Journals (Sweden)

    Jucelio Peter Duarte

    2015-03-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2015v28n2p103 Periplaneta americana is a species of great importance to public health, since it can act as a vector of many pathogens and it reaches large populations in urban environments. This is probably due to its ability to resist starvation and desiccation. This study aimed to evaluate the effects of absence of water and food on survival and weight change among adult P. americana individuals and check whether the initial weight of individuals influences on their survival. Four groups having twenty P. americana couples were formed and subject to: I no water or food; II no food; III no water; and IV control group. Insects were isolated according to the groups, which were weighed at the beginning and end of the stress conditions. They remained under these conditions until all individuals in each test group were dead. Stress conditions caused reduction in survival time when compared to the control group. Adults with higher body mass survived longer when deprived only of food, while among those lacking water, weight had no influence on survival. Total weight loss was greater among individuals deprived of water than those deprived only of food.

  16. IN VITRO CYTOTOXICITY STUDY OF AGAVE AMERICANA, STRYCHNOS NUX-VOMICA AND ARECA CATECHU EXTRACTS USING MCF-7 CELL LINE

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    Anajwala Chetan C.

    2010-06-01

    Full Text Available Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nux-vomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water. By trypan blue dye exclusion method, Viability of MCF-7 and Vero cell lines were 85.50 and 81.13%, respectively. IC50 value of methanol extract of Agave americana leaves and aqueous extract of Areca catechu fruits were found to be 545.9 & 826.1 µg/ml by SRB assay and 775.1 & 1461µg/ml by MTT assay, respectively, against MCF-7 cell line. From cytotoxicity study data by SRB and MTT assay, it revealed that methanol extract of Agave americana and aqueous extract of Areca catechu are potent cytotoxic.

  17. Phylogenomic analysis of Odyssella thessalonicensis fortifies the common origin of Rickettsiales, Pelagibacter ubique and Reclimonas americana mitochondrion.

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    Kalliopi Georgiades

    Full Text Available BACKGROUND: The evolution of the Alphaproteobacteria and origin of the mitochondria are topics of considerable debate. Most studies have placed the mitochondria ancestor within the Rickettsiales order. Ten years ago, the bacterium Odyssella thessalonicensis was isolated from Acanthamoeba spp., and the 16S rDNA phylogeny placed it within the Rickettsiales. Recently, the whole genome of O. thessalonicensis has been sequenced, and 16S rDNA phylogeny and more robust and accurate phylogenomic analyses have been performed with 65 highly conserved proteins. METHODOLOGY/PRINCIPAL FINDINGS: The results suggested that the O. thessalonicensis emerged between the Rickettsiales and other Alphaproteobacteria. The mitochondrial proteins of the Reclinomonas americana have been used to locate the phylogenetic position of the mitochondrion ancestor within the Alphaproteobacteria tree. Using the K tree score method, nine mitochondrion-encoded proteins, whose phylogenies were congruent with the Alphaproteobacteria phylogenomic tree, have been selected and concatenated for Bayesian and Maximum Likelihood phylogenies. The Reclinomonas americana mitochondrion is a sister taxon to the free-living bacteria Candidatus Pelagibacter ubique, and together, they form a clade that is deeply rooted in the Rickettsiales clade. CONCLUSIONS/SIGNIFICANCE: The Reclinomonas americana mitochondrion phylogenomic study confirmed that mitochondria emerged deeply in the Rickettsiales clade and that they are closely related to Candidatus Pelagibacter ubique.

  18. Biochemical and molecular modulation of CCl4-induced peripheral and central damage by Tilia americana var. mexicanaextracts.

    Science.gov (United States)

    Coballase-Urrutia, Elvia; Cárdenas-Rodríguez, Noemí; González-García, María Carolina; Núñez-Ramírez, Eithan; Floriano-Sánchez, Esaú; González-Trujano, María Eva; Fernández-Rojas, Berenice; Pedraza-Chaverrí, José; Montesinos-Correa, Hortencia; Rivera-Espinosa, Liliana; Sampieri, Aristides Iii; Carmona-Aparicio, Liliana

    2017-03-01

    Around the world, species from the genus Tilia are commonly used because of their peripheral and central medicinal effects; they are prepared as teas and used as tranquilizing, anticonvulsant, and analgesic agents. In this study, we provide evidence of the protective effects of organic and aqueous extracts (100 mg/kg, i.p.) obtained from the leaves of Tilia americana var. mexicana on CCl 4 -induced liver and brain damage in the rat. Protection was observed in the liver and brain (cerebellum, cortex and cerebral hemispheres) by measuring the activity of antioxidant enzymes and levels of malondialdehyde (MDA) using spectrophotometric methods. Biochemical parameters were also assessed in serum samples from the CCl 4 -treated rats. The T. americana var. mexicana leaf extracts provided significant protection against CCl 4 -induced peripheral and central damage by increasing the activity of antioxidant enzymes, diminishing lipid peroxidation, and preventing alterations in biochemical serum parameters, such as the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-globulin (γ-GLOB), serum albumin (ALB), total bilirubin (BB), creatinine (CREA) and creatine kinase (CK), relative to the control group. Additionally, we correlated gene expression with antioxidant activity in the experimental groups treated with the organic and aqueous Tilia extracts and observed a non-statistically significant positive correlation. Our results provide evidence of the underlying biomedical properties of T. americana var. mexicana that confer its neuro- and hepatoprotective effects.

  19. Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.

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    Neyser De La Torre-Ruiz

    Full Text Available ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA, solubilize phosphate, and had nitrogenase activity. Inoculation using the plant growth-promoting bacteria strains had a significant effect (p < 0.05 on plant growth and the sugar content of A. americana, showing that these native plant growth-promoting bacteria are a practical, simple, and efficient alternative to promote the growth of agave plants with proper biological characteristics for agroindustrial and biotechnological use and to increase the sugar content in this agave species.

  20. Um olhar sistêmico sobre a crise norte-americana

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    CHRISTIAN CARVALHO GANZERT

    Full Text Available RESUMO No contexto contemporâneo, a economia global pode ser descrita como um sistema baseado em agentes que, quando associados, manifestam seus próprios meios e fins. A crise das hipotecas subprime nos Estados Unidos é um bom exemplo das implicações desse tipo de relacionamento. Ela está diretamente relacionada a poderosos laços de realimentação, compostos por uma série de variáveis que amplificaram o fato de as famílias americanas terem se endividado em ritmo maior do que a sua distribuição de riqueza, prejudicada pela externalização da produção. Há atualmente uma nova estrutura na economia internacional, em que as ex-nações subdesenvolvidas estão mais bem posicionadas no cenário macroeconômico e têm maior poder do que antes - uma consequência do necessário rearranjo sistêmico. Diante dessa complexa dinâmica, a função de inteligência que deveria controlar e garantir a estabilidade sistêmica falhou em perceber a dinâmica e os impactos da nova cultura atrelada ao comportamento do capital contemporâneo, para evitar a perda da hegemonia. Observando-se ex post facto, por meio de um enfoque sistêmico, a crise econômica americana pode ser definida como tendo suas raízes nas premissas de todo o arcabouço cultural da globalização que acabou por conduzir à externalização da produção. Assim, o contexto sistêmico desta análise aponta que a cultura, como importante gerador e amplificador, deve ser o principal foco dos intentos de análise do atual contexto socioeconômico global.

  1. Effect of an admixture from Agave americana on the physical and mechanical properties of plaster

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    Ochoa, J. C.

    2013-03-01

    Full Text Available Physical and mechanical properties of a plaster paste added with an organic admixture, of the leaves of Agave americana, were studied. Plastic consistency behavior was evaluated and the water/gypsum(w/g ratio was determined for each dosage of the admixture. Admixtur eeffect on setting was evaluated too. The chemical transformation of the hemihydrated form to gypsum (dihydrated form was studied using a novel technique based on a moisture analyzer by halogen light. Flexural and compressive strengths were measured. The results show that ,for the same consistency, accordingly mechanical strengths were improved too. The setting times were increased which would enhance the application time of plaster and would reduce plastic shrinkage, common problems in this type of material. The changes in these physical properties not substantially affect the final mechanical strengths.

    Se estudiaron algunas propiedades físicas y mecánicas de pastas de yeso de construcción adicionadas con un aditivo de origen orgánico, producto de las hojas de la planta Agave americana. Se evaluó el comportamiento plástico de la pasta mediante ensayos de consistencia y se determinó, para cada dosificación, su relación agua/ yeso (a/y. Se evaluó la incidencia del aditivo en los tiempos de fraguado. La transformación química del hemihidrato a yeso dihidrato se estudió mediante una novedosa técnica basada en un analizador de humedad por luz halógena. Se midieron las resistencias mecánicas a flexo-tracción y a compresión. Los resultados del estudio muestran que, para una misma consistencia, se mejoran las resistencias mecánicas. Los tiempos de fraguado de la pasta se aumentaron lo que ayudaría mejorar los tiempos de aplicación del yeso y a disminuir las retracciones plásticas. Las modificaciones de estas propiedades físicas no afectan considerablemente las resistencias mecánicas finales.

  2. Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes

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    Ricardo José Gunski

    1998-06-01

    Full Text Available Sequential banding analysis (Giemsa-C-banding-Ag NOR of chromosomes of the common rhea (Rhea americana was performed. Metaphases were obtained by peripheral blood lymphocyte culture and monolayer embryo cell culture. The diploid chromosome number was 80, different from the 2n = 82 in previous reports. Macrochromosome pairs 1, 2 and 5 were submetacentric and pair 3, subacrocentric. The 4th pair was acrocentric and all of the microchromosomes appeared to be acrocentric, with the exception of a clearly metacentric pair which was fully heterochromatic. The Z was slightly larger than the W, both being acrocentric and C-band negative. Nucleolar organizer regions were observed in the secondary constriction of a microchromosome pair. Correct identification of the NOR-bearing pair was possible only by sequential analyses, Giemsa staining followed by the Ag-NOR technique.Foram efetuadas análises seqüenciais de bandeamento cromossômico (Giemsa-banda-C-AgNOR em material da espécie Rhea americana (ema com o objetivo de identificar os cromossomos portadores de regiões organizadoras de nucléolos e confirmar o cariótipo desta espécie. As metáfases foram obtidas de culturas de leucócitos e de células de embrião. O número diplóide de cromossomos, determinado pela análise de metáfases oriundas de 19 espécimes, foi de 80 (2n = 80, NF = 95, o que difere da literatura. Os pares de macrocromossomos números 1, 2 e 5 eram submetacêntricos e o par 3 era sub-acrocêntrico, confirmado pelo bandeamento C. O par 4 era acrocêntrico, bem como todos os microcromossomos, com exceção de um metacêntrico inteiramente heterocromático. O cromossomo Z era ligeiramente maior que o W, sendo ambos acrocêntricos e banda-C negativos. A região organizadora de nucléolos foi observada na constrição secundária de um par de microcromossomos. A correta identificação do par portador da NOR só foi possível com a utilização da análise seqüencial de colora

  3. Cinética de secagem e difusividade efetiva em folhas de jenipapo (Genipa americana L.

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    L.A. SILVA

    2015-01-01

    Full Text Available RESUMO O jenipapo (Genipa americana L. é uma espécie nativa com importância medicinal, sendo amplamente utilizada no Brasil. Em função da necessidade de conhecimento à cerca do pré-processamento desta espécie, este trabalho foi desenvolvido com o objetivo de avaliar a cinética de secagem de folhas de jenipapo (G. americana L., bem como determinar a difusividade efetiva da água durante o processo. As folhas foram coletadas com teor de água inicial de 2,30±0,05 (decimal b.s., e submetidas à secagem em três condições de temperatura do ar (35,3; 46,0 e 65,0°C até atingirem o teor de água de equilíbrio. Aos dados experimentais, ajustaram-se doze modelos matemáticos, recomendados para representar o processo de secagem de produtos agrícolas. As magnitudes do coeficiente de determinação (R2, do erro médio relativo (P, do erro médio estimado (SE e do teste do qui-quadrado (χ2, foram utilizadas para verificar o grau de ajuste dos modelos. Os modelos de Henderson e Pabis modificado e Midilli apresentaram ajustes adequados aos dados experimentais, sendo o modelo de Midilli, em função de sua simplicidade, escolhido para representar a cinética de secagem das folhas de jenipapo. Aumentando a temperatura do ar de secagem de 35,3 para 46,0 e 65,0ºC houve redução no tempo de secagem das folhas de jenipapo de 91,1 para 62,5 e 24,2 horas, respectivamente. O coeficiente de difusão efetivo aumenta com a elevação da temperatura, e esta relação é descrita pela equação de Arrhenius, que apresenta energia de ativação para a difusão líquida de 33,9 kJ mol-1.

  4. Effects of freezing on white perch Morone americana (Gmelin, 1789): Implications for multivariate morphometrics

    Science.gov (United States)

    Kocovsky, Patrick

    2016-01-01

    This study tested the hypothesis that duration of freezing differentially affects whole-body morphometrics of a derived teleost. Whole-body morphometrics are frequently analyzed to test hypotheses of different species, or stocks within a species, of fishes. Specimens used for morphometric analyses are typically fixed or preserved prior to analysis, yet little research has been done on how fixation or preservation methods or duration of preservation of specimens might affect outcomes of multivariate statistical analyses of differences in shape. To determine whether whole-body morphometrics changed as a result of freezing, 23 whole-body morphometrics of age-1 white perch (Morone americana) from western Lake Erie (n = 211) were analyzed immediately after capture, after being held on ice overnight, and after freezing for 100 or 200 days. Discriminant function analysis revealed that all four groups differed significantly from one another (P time to avoid biases related to the length of time they were frozen. Similar experiments should be conducted on other species and also using formalin- and alcohol-preserved specimens.

  5. Comparison of Serum Protein Electrophoresis Values in Wild and Captive Whooping Cranes ( Grus americana ).

    Science.gov (United States)

    Hausmann, Jennifer C; Cray, Carolyn; Hartup, Barry K

    2015-09-01

    Protein electrophoresis of serum samples from endangered, wild whooping cranes ( Grus americana ) was performed to help assess the health of the only self-sustaining, migratory population in North America. Serum samples from wild adult cranes (n = 22) were taken at Aransas National Wildlife Refuge, Texas, USA during winter. Wild juvenile cranes (n = 26) were sampled at Wood Buffalo National Park, Northwest Territories, Canada, in midsummer. All captive crane samples were acquired from the International Crane Foundation, Baraboo, WI, USA. Captive adult cranes (n = 30) were sampled during annual examinations, and archived serum samples from captive juvenile cranes (n = 19) were selected to match the estimated age of wild juveniles. Wild juveniles had significantly lower concentrations of all protein fractions than wild adults, except for prealbumin and γ globulins. All protein fraction concentrations for wild juveniles were significantly lower compared with captive juveniles, except for prealbumin and γ globulins, which were higher. Wild adults had significantly greater γ globulin concentrations than captive adults. Captive juveniles had significantly lower prealbumin and albumin concentrations and albumin : globulin ratios than captive adults. The higher γ globulin concentrations in wild versus captive cranes are likely because of increased antigenic exposure and immune stimulation. Protein fraction concentrations vary significantly with age and natural history in this species. Reference intervals for serum protein electrophoresis results from captive adult whooping cranes are provided in this study.

  6. Potential risk to wood storks (Mycteria americana) from mercury in Carolina Bay fish

    International Nuclear Information System (INIS)

    Brant, H.A.; Jagoe, C.H.; Snodgrass, J.W.; Bryan, A.L.; Gariboldi, J.C.

    2002-01-01

    Fish mercury levels from some Carolina bays pose risk to wood stork. - Carolina bays are freshwater wetlands that serve as important feeding habitats for the endangered wood stork (Mycteria americana). Water levels in these bays fluctuate greatly and tend to be acidic and rich in dissolved organic carbon (DOC), factors that favor mercury (Hg) methylation and bioaccumulation in fish. To assess potential risks to wood storks consuming mercury contaminated fish in bays, we sampled fish from 10 bays on the Savannah River Site (SRS), South Carolina, an area with documented use by wood storks. Whole body mercury concentrations in 258 fishes of three species (Erimyzon sucetta, Acantharchus pomotis and Esox americanus) commonly consumed by wood storks were determined. Risk factors for nestlings and free-ranging adults were calculated using published no and lowest observable adverse effect concentration (NOAEC and LOAEC) values for birds. Fish from higher trophic levels and those from wetlands with relatively shallow maximum depths and fluctuating water levels were more likely to exceed NOAEC and LOAEC values. Calculation of exposure rates of nestling wood storks indicated they are at highest risk during the first 10 days of the nestling period. These calculations suggest that there is potential concern for wood storks foraging in relatively shallow bays with fluctuating water levels, even though there is no obvious local source of mercury to these wetlands

  7. Seasonal movements and multiscale habitat selection of Whooping Crane (Grus americana) in natural and agricultural wetlands

    Science.gov (United States)

    Pickens, Bradley A.; King, Sammy L.; Vasseur, Phillip L.; Zimorski, Sara E.; Selman, Will

    2017-01-01

    Eleven of 15 species of cranes (family: Gruidae) are considered vulnerable or endangered, and the increase of agriculture and aquaculture at the expense of natural wetlands and grasslands is a threat to Gruidae worldwide. A reintroduced population of Whooping Crane (Grus americana) was studied in coastal and agricultural wetlands of Louisiana and Texas, USA. The objectives were to compare Whooping Crane movements across seasons, quantify multiscale habitat selection, and identify seasonal shifts in selection. Whooping Cranes (n = 53) were tracked with satellite transmitters to estimate seasonal core-use areas (50% home range contours) via Brownian bridge movement models and assess habitat selection. Whooping Crane core-use areas (n = 283) ranged from 4.7 to 438.0 km2, and habitat selection changed seasonally as shallow water availability varied. Whooping Crane core-use areas were composed of more fresh marsh in spring/summer, but shifted towards rice and crawfish (Procambarus spp.) aquaculture in the fall/winter. Within core-use areas, aquaculture was most strongly selected, particularly in fall when fresh marsh became unsuitable. Overall, the shifting of Whooping Crane habitat selection over seasons is likely to require large, heterogeneous areas. Whooping Crane use of agricultural and natural wetlands may depend on spatio-temporal dynamics of water depth.

  8. Plantio direto de alface americana sobre plantas de cobertura dessecadas ou roçadas

    Directory of Open Access Journals (Sweden)

    Andréia Cristina Silva Hirata

    2014-06-01

    Full Text Available O objetivo deste trabalho foi avaliar o plantio direto de alface americana no verão sobre plantas de cobertura dessecadas ou roçadas em cultivos sucessivos. O trabalho foi desenvolvido no município de Álvares Machado, São Paulo, Brasil. O delineamento experimental foi em blocos ao acaso, dispostos em parcelas subdivididas, com quatro repetições. O fator da parcela principal foram dois manejos das plantas de cobertura (dessecadas com herbicida ou roçadas e as subparcelas, seis espécies de plantas de cobertura (Cajanus cajan cv. IAPAR 43, Crotalaria spectabilis, Crotalaria juncea, Mucuna pruriens, Pennisetum glaucum e vegetação natural, além da testemunha sem plantas de cobertura. O manejo roçado apresentou desempenho inferior no primeiro cultivo da alface, todavia não diferiu do manejo químico no segundo cultivo. A cobertura do solo com mucuna apresentou maior produtividade da alface no primeiro cultivo devido ao excesso de palha das demais coberturas, o qual prejudicou o estabelecimento da alface. No segundo cultivo não houve diferenças entre as coberturas. A roçagem de plantas de cobertura é uma opção viável para plantio direto de alface sem herbicidas.

  9. Los aportes de una antropología americana en el pensamiento de Rodolfo Kusch

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    Dina Picotti

    2010-01-01

    Full Text Available El presente texto versa sobre la propuesta de Rodolfo Kusch en torno a una antropología del hombre americano a partir de un recorrido por algunas obras insignes de su pensamiento antropológico. El trabajo parte de la pro - blematización de la categoría de situación. A partir de ciertas distinciones filosóficas establecidas entre ser y estar , la autora aborda la cuestión de la identidad americana como pueblo , para indicar la manera en que el habla popular, en su nombrar y callar, es la base de lo que pueda ser el hombre ame - ricano. Después de la descripción de la consolidación del hombre americano tras el dualismo mito-razón y la formulación de la inconmensurabilidad del hombre americano ante criterios que resultan discordes, se sugiere otro modo de pensar, en este caso, de concebir una antropología, otra línea de encarar lo humano en relación con el orden vigente. De esta manera, se postula la idea de que el problema de América es el de recuperar toda la potencialidad del pensar y saber apostar al futuro, lo cual se realiza a partir de la revisión de los niveles económicos, educativos y artísticos del hombre americano.

  10. Transcriptome analysis of Phytolacca americana L. in response to cadmium stress.

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    Yongkun Chen

    Full Text Available Phytolacca americana L. (pokeweed has metal phytoremediation potential, but little is known about its metal accumulation-related genes. In this study, the de novo sequencing of total RNA produced 53.15 million reads covering 10.63 gigabases of transcriptome raw data in cadmium (Cd-treated and untreated pokeweed. Of the 97,502 assembled unigenes, 42,197 had significant matches in a public database and were annotated accordingly. An expression level comparison between the samples revealed 1515 differentially expressed genes (DEGs, 923 down- and 592 up-regulated under Cd treatment. A KEGG pathway enrichment analysis of DEGs revealed that they were involved in 72 metabolism pathways, with photosynthesis, phenylalanine metabolism, ribosome, phenylpropanoid biosynthesis, flavonoid biosynthesis and carbon fixation in photosynthetic organisms containing 24, 18, 72, 14, 7 and 15 genes, respectively. Genes related to heavy metal tolerance, absorption, transport and accumulation were also identified, including 11 expansins, 8 nicotianamine synthases, 6 aquaporins, 4 ZRT/IRT-like proteins, 3 ABC transporters and 3 metallothioneins. The gene expression results of 12 randomly selected DEGs were validated using quantitative real-time PCR, and showed different response patterns to Cd in their roots, stems and leaves. These results may be helpful in increasing our understanding of heavy metal hyperaccumulators and in future phytoremediation applications.

  11. Estudios sobre Trypanosomiasis americana en el Perú. Observaciones en el departamento de Ica

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    Víctor M Ayulo Robles

    1946-01-01

    Full Text Available Hemos comprobado la presencia de Triatomas en las ciudades de Ica y Nazca. Los Triatomas encontrados en esas localidades son de la especie del Triatoma Infestans. Las observaciones del contenido intestinal de los Triatomas capturados en ambas ciudades reveló que ellos no se encuentran infestados; lo que hace sospechar que estos insectos procedan de la Ciudad de Arequipa. Los exámenes de sangre en fresco, gota gruesa y frotis coloreados por el método de Giemsa en 174 personas y 32 animales, así como las pruebas de Desviación del Complemento (Reacción de GUERREIRO Y MACHADO e intradermo-reacción en las 174 personas, fueron negativas; lo que demuestra que actualmente no existe la Enfermedad de Chagas en el Departamento de Ica. Dada la presencia de Triatomas en Ica y Nazca y la existencia de la Enfermedad de Chagas en Vitor, Sihuas y Quishuarani del Departamento de Arequipa no se excluye la posibilidad de que posteriormente se haga presente la Trypanosomiasis americana, también, en esas zonas.

  12. “Ser devorado no duele”. Th. W. Adorno y la experiencia americana

    Directory of Open Access Journals (Sweden)

    Maiso, Jordi

    2009-10-01

    Full Text Available The present text aims to fathom Theodor W. Adorno’s american experience as a deciding learning process in the development of his Critical Theory. As a consequence of his New York exile years and his participance on the Princeton Radio Research Project, Adorno not only gets in touch with the proceeding of the culture industry and sets down a resolute theoretical approach to radio music, but also realizes a shift has taken place on the social function of theory which enables him a poignant self-reflection on the situation of the emigrated intellectual.El presente texto pretende desentrañar la experiencia americana de Theodor W. Adorno como un proceso de aprendizaje decisivo en la formulación de su Teoría Crítica. A partir de su exilio en Nueva York y de su participación en el Princeton Radio Research Project, Adorno no sólo adquiere un conocimiento directo del funcionamiento de la industria de la cultura y formula una decisiva aproximación teórica a la música radiofónica, sino que también toma conciencia de un cambio en la función social de la teoría que le permite llevar a cabo una aguda autorreflexión sobre la situación del intelectual emigrado.

  13. Isolation and Purification of an Antibacterial Protein from Immune Induced Haemolymph of American Cockroach, Periplaneta americana

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    Hamid Reza Basseri

    2016-10-01

    Full Text Available Background: Antimicrobial peptides play a role as effectors substances in the immunity of vertebrate and inverte­brate hosts. In the current study, antimicrobial peptide was isolated from the haemolymph of the American cock­roach, Periplaneta americana.Methods: Micrococcus luteus as Gram-positive bacteria and Escherichia coli as Gram-negative bacteria were candi­date for injection. Induction was done by injecting both bacteria into the abdominal cavity of two groups of cock­roaches separately. The haemolymphs were collected 24 hours after post injection and initially tested against both bacteria. Subsequently, the immune induced haemolymph was purified by high performance liquid chromatography (HPLC to separate the proteins responsible for the antibacterial activity.Results: The non-induced haemolymph did not show any activity against both bacteria whereas induced haemo­lymph exhibited high activity against M. luteus but did less against E. coli. Two fractions showed antibacterial activ­ity against M. luteus. Finally the molecular weight of the isolated antibacterial proteins were determined as 72 kDa and 62 kDa using SDS-PAGE.Conclusion: Induced haemolymph of American cockroaches has the ability to produce peptides to combat against Gram-positive bacteria when an immune challenge is mounted. Further work has to be done to sequence of the pro­tein, which it would be advantageous.

  14. De la independencia a la natural barbarie americana. Una lectura del Facundo de Domingo F. Sarmiento

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    Maura Brighenti

    2010-01-01

    Full Text Available Se presenta una lectura del "Facundo" de Domingo F. Sarmiento y, en particular, de su más general visión de la historia y de la realidad argentina y latinoamericana a partir del esquema dicotómico de civilización y barbarie que tendrá mucha suerte en las narraciones de los procesos del modernización latinoamericanos por lo menos hasta la segunda mitad del siglo XX. A través del análisis de algunos entre los múltiples niveles narrativos del "Facundo" se quiere mostrar como tal imagen dicotómica se va cristalizando en el texto. Partiendo del espacio infinito y vacío de la pampa y pasando por una antropología del gaucho - de su manera de vivir y de conducir la guerra - Sarmiento conduce el lector hasta la forma típicamente americana de despotismo, el caudillismo, llegado en Argentina a su epopeya final con el régimen de Juan M. de Rosas. En la última parte se intenta injertar el esquema dicotómico del Facundo al interior de la más general reflexión del Occidente sobre la modernidad y sus alteridades, a través por un lado del prisma orientalista y por el otro de las mismas fuentes del pensamiento moderno europeo y en particular de Locke y Hobbes.

  15. Subcellular distribution and chemical forms of cadmium in Phytolacca americana L

    Energy Technology Data Exchange (ETDEWEB)

    Fu Xiaoping; Dou Changming [Ministry of Agriculture Key Laboratory of Non-point Source Pollution Control, Institute of Environmental Science and Technology, Zhejiang University, Hangzhou 310029 (China); Chen Yingxu, E-mail: yingxu_chen@hotmail.com [Ministry of Agriculture Key Laboratory of Non-point Source Pollution Control, Institute of Environmental Science and Technology, Zhejiang University, Hangzhou 310029 (China); Chen Xincai; Shi Jiyan; Yu Mingge; Xu Jie [Ministry of Agriculture Key Laboratory of Non-point Source Pollution Control, Institute of Environmental Science and Technology, Zhejiang University, Hangzhou 310029 (China)

    2011-02-15

    Phytolacca americana L. (pokeweed) is a promising species for Cd phytoextraction with large biomass and fast growth rate. To further understand the mechanisms involved in Cd tolerance and detoxification, the present study investigated subcellular distribution and chemical forms of Cd in pokeweed. Subcellular fractionation of Cd-containing tissues indicated that both in root and leaves, the majority of the element was located in soluble fraction and cell walls. Meanwhile, Cd taken up by pokeweed existed in different chemical forms. Results showed that the greatest amount of Cd was found in the extraction of 80% ethanol in roots, followed by 1 M NaCl, d-H{sub 2}O and 2% HAc, while in leaves and stems, most of the Cd was extracted by 1 M NaCl, and the subdominant amount of Cd was extracted by 80% ethanol. It could be suggested that Cd compartmentation with organo-ligands in vacuole or integrated with pectates and proteins in cell wall might be responsible for the adaptation of pokeweed to Cd stress.

  16. Food habits of stunted and non-stunted white perch (Morone americana)

    Science.gov (United States)

    Gosch, N.J.C.; Stittie, J.R.; Pope, K.L.

    2010-01-01

    We studied food habits of white perch (Morone americana) from two populations with different stable states (stunted [Branched Oak Lake, Nebraska] and nonstunted [Pawnee Lake, Nebraska]) to determine if change in food habits of white perch is likely to occur in situations where a stunted white perch population is altered to a nonstunted state and vice versa. Three approaches were used to quantitatively describe seasonal (spring = March-May, summer = June-August, autumn = September-November) diets of white perch - 1) frequency of occurrence, 2) percentage of composition by volume, and 3) mean stomach fullness. White perch diets were dominated by cladocerans and dipterans in both reservoirs during all seasons. Fish egg predation was similar between reservoirs, and white perch rarely consumed fishes in either the stunted or the non-stunted population. Shifting a white perch population between stunted and non-stunted states will likely cause little or no change in food habits; fish in both states will primarily consume invertebrates.

  17. Fast and Powerful: Biomechanics and Bite Forces of the Mandibles in the American Cockroach Periplaneta americana.

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    Tom Weihmann

    Full Text Available Knowing the functionality and capabilities of masticatory apparatuses is essential for the ecological classification of jawed organisms. Nevertheless insects, especially with their outstanding high species number providing an overwhelming morphological diversity, are notoriously underexplored with respect to maximum bite forces and their dependency on the mandible opening angles. Aiming for a general understanding of insect biting, we examined the generalist feeding cockroach Periplaneta americana, characterized by its primitive chewing mouth parts. We measured active isometric bite forces and passive forces caused by joint resistance over the entire mandibular range with a custom-built 2D force transducer. The opening angle of the mandibles was quantified by using a video system. With respect to the effective mechanical advantage of the mandibles and the cross-section areas, we calculated the forces exerted by the mandible closer muscles and the corresponding muscle stress values. Comparisons with the scarce data available revealed close similarities of the cockroaches' mandible closer stress values (58 N/cm2 to that of smaller specialist carnivorous ground beetles, but strikingly higher values than in larger stag beetles. In contrast to available datasets our results imply the activity of faster and slower muscle fibres, with the latter becoming active only when the animals chew on tough material which requires repetitive, hard biting. Under such circumstances the coactivity of fast and slow fibres provides a force boost which is not available during short-term activities, since long latencies prevent a specific effective employment of the slow fibres in this case.

  18. Morphogenesis of the rhea (Rhea americana respiratory system in different embryonic and foetal stages

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    Renata P. Sousa

    Full Text Available ABSTRACT: The rhea (Rhea americana is an important wild species that has been highlighted in national and international livestock. This research aims to analyse embryo-foetal development in different phases of the respiratory system of rheas. Twenty-three embryos and foetuses were euthanized, fixed and dissected. Fragments of the respiratory system, including the nasal cavity, larynx, trachea, syrinx, bronchi and lungs, were collected and processed for studies using light and scanning electron microscopy. The nasal cavity presented cubic epithelium in the early stages of development. The larynx exhibited typical respiratory epithelium between 27 and 31 days. The trachea showed early formation of hyaline cartilage after 15 days. Syrinx in the mucous membrane of 18-day foetuses consisted of ciliated epithelium in the bronchial region. The main bronchi had ciliated epithelium with goblet cells in the syringeal region. In the lung, the parabronchial stage presented numerous parabronchi between 15 and 21 days. This study allowed the identification of normal events that occur during the development of the rhea respiratory system, an important model that has not previously been described. The information generated here will be useful for the diagnosis of pathologies that affect this organic system, aimed at improving captive production systems.

  19. Molecular characterization and localization of the first tyramine receptor of the American cockroach (Periplaneta americana).

    Science.gov (United States)

    Rotte, C; Krach, C; Balfanz, S; Baumann, A; Walz, B; Blenau, W

    2009-09-15

    The phenolamines octopamine and tyramine control, regulate, and modulate many physiological and behavioral processes in invertebrates. Vertebrates possess only small amounts of both substances, and thus, octopamine and tyramine, together with other biogenic amines, are referred to as "trace amines." Biogenic amines evoke cellular responses by activating G-protein-coupled receptors. We have isolated a complementary DNA (cDNA) that encodes a biogenic amine receptor from the American cockroach Periplaneta americana, viz., Peatyr1, which shares high sequence similarity to members of the invertebrate tyramine-receptor family. The PeaTYR1 receptor was stably expressed in human embryonic kidney (HEK) 293 cells, and its ligand response has been examined. Receptor activation with tyramine reduces adenylyl cyclase activity in a dose-dependent manner (EC(50) approximately 350 nM). The inhibitory effect of tyramine is abolished by co-incubation with either yohimbine or chlorpromazine. Receptor expression has been investigated by reverse transcription polymerase chain reaction and immunocytochemistry. The mRNA is present in various tissues including brain, salivary glands, midgut, Malpighian tubules, and leg muscles. The effect of tyramine on salivary gland acinar cells has been investigated by intracellular recordings, which have revealed excitatory presynaptic actions of tyramine. This study marks the first comprehensive molecular, pharmacological, and functional characterization of a tyramine receptor in the cockroach.

  20. The role of gut microbiota in the regulation of standard metabolic rate in female Periplaneta americana

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    Paul A. Ayayee

    2018-05-01

    Full Text Available Insect gut microbiota contribute significantly to host nutritional ecology. Disrupting insect gut microbial assemblages impacts nutrient provisioning functions, and can potentially affect host standard metabolic rate (SMR, a measure of host energy balance. In this study, we evaluated the effect of disrupting gut microbial assemblages on the SMR of female Periplaneta americana cockroaches fed dog food (DF, high protein/carbohydrate (p/c ratio, and cellulose-amended dog food (CADF, 30% dog food, 70% cellulose, low p/c ratio diets, supplemented with none, low, or high antibiotic doses. Bacterial loads decreased significantly between diet types (P = 0.04 and across antibiotic doses (P = 0.04. There was a significant diet type x antibiotic dose interaction on SMR of females on both diets (P = 0.05 by the end of the seven-day experimental period. In CADF-fed females, SMR decreased linearly with decreasing bacterial load. However, SMR of DF-fed females on the low dose was significantly higher than those in the control and high dose groups. This is interpreted as a diet-dependent response by low dose DF-fed females to the loss of nutritional services provided by gut bacteria. Severe reductions in bacterial load at high doses reduced SMR of females on both diet types. This study provides insights into the potential role of gut bacteria as modulators of host energy expenditure under varying dietary conditions.

  1. Behavioral responses of Schistocerca americana (Orthoptera: Acrididae) to Azadirex (neem)-treated host plants.

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    Capinera, John L; Froeba, Jason G

    2007-02-01

    Azadirex (azadirachtin and other biologically active extracts from neem trees) has been shown to have considerable potential to be used in integrated pest management systems based on its growth regulator/insecticide properties. Less well known are the antifeedant properties. The feeding-deterrent properties of a commercial azadirex formulation (Azatrol EC) were evaluated using both no-choice and choice tests, the American grasshopper, Schistocerca americana (Drury), and four host plants [savoy cabbage, Brassica oleracea variety capitata L.; cos (romaine) lettuce, Lactuca sativa variety longifolia Lam.; sweet orange, Citrus sinensis variety Hamlin L.; and peregrina, Jatropha integerrima Jacq.]. These studies demonstrated that azadirex application can significantly affect the feeding behavior of grasshoppers. Some degree of protection can be afforded to plants that differ markedly in their innate attractiveness to the insect, although the level of protection varies among hosts. The tendency of grasshoppers to sometimes feed on azadirex-treated foliage suggests that it will be difficult to prevent damage from occurring at all times, on all hosts. No evidence of rapid habituation to azadirex was detected. Rapid loss of efficacy was observed under field conditions, suggesting that daily retreatment might be necessary to maintain protection of plants from feeding.

  2. GIBBERELLINS, FUNGICIDES AND STORAGE EFFECTS ON THE GERMINATION OF Genipa americana L. (RUBIACEAE SEEDS

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    Fábio de Almeida Vieira

    2006-06-01

    Full Text Available The aim of this paper was to verify the effect of different doses of gibberellic acid (GA3 (0, 250, 500, 750 and 1000 µg.L-1, of fungicides of the groups chemical benzimidazol (0, 25, 50 and 100 g.L-1 and ditiocarbamato (0, 1,25, 2,50 and 5,00 g.L-1 on seed germination. Viability of those seeds was evaluated through germination tests at 0, 15, 30 and 60 days. The experiment was carried out in greenhouse. The experimental design was fully randomized one, with five replicates per treatment. The traits evaluated were emergence and index of emergence speed. The treatment with GA3 didn't provide significant so much differences among the germination rates as well as for the emergence speed. It was verified that the use of the fungicides in smaller concentrations (25 g.L-1 of benzimidazol and 1,25 g.L-1 of ditiocarbamato promoted a better germination speed. The seeds of G. americana possess viability period relatively short, with germination absence 60 days period of storage, and it could be associated to the humidity tenors presented by the seeds in this period.

  3. Anatomical characterization of ultra-structures, biominerals and histolocalization of metabolites in leaves of Genipa americana

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    Alex L. Vasconcelos

    Full Text Available Abstract Inserted in one of the largest families among the Angiosperms, Genipa americana L., Rubiaceae, can be found in all Brazilian territory, presenting great medicinal importance, where several uses have been attributed. In view of this, this work has the purpose of analyzing the ultrastructural, biomineral, phytochemical and histochemical characteristics of the leaves of this native species from Brazil. For this, light microscopy, polarization and scanning electron microscopy techniques were used with X-ray scattering energy, associated to chromatographic and histochemical tests. The anatomical ultrastructural characteristics of the leaves detailed information about the type and arrangement of the cuticle, trichomes, surface and arrangement of the tissues that determine the botanical identity of this species. The phytochemical tests allowed determining their chromatographic pattern and histochemistry to determine the exact storage site of these substances in the leaf. It was observed that the characterization of the crystalline macro-pattern present in the analyzed species, as well as its exact elemental composition, can be considered an important differential diagnosis factor. The results characterize the leaves of this species in different aspects, being a native species and pharmacologically promising, with different popular uses and proven pharmacological activities, and more in depth studies is needed.

  4. Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL. λ using the prophage induction test (SOS inductest

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    Juliana Brandstetter Vilar

    2009-09-01

    Full Text Available Curatella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS inductest. To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the extract, Escherichia coli WP2s(λ cultures were diluted in M9 buffer, inoculated into LB plates, and incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ was treated with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain (RJF013, LB(1/2(malt/amp, seeded into plates with the matches, and incubated for 24 h at 37 °C. After this period, the total number of colonies and the number of plaques were counted to evaluate C. americana cytotoxicity and genotoxicity, respectively. Our results showed that although the extract of "lixeira" did not modify the survival of bacteria (p > 0.05, it caused a significant increase in prophage λ induction, especially at the higher concentrations (pCuratella americana L., comumente conhecida como "lixeira" no Brasil, é utilizada em medicina popular para tratamento de úlceras e inflamações. O presente trabalho teve como objetivo avaliar o potencial citotóxico e genotóxico do extrato etanólico das cascas de C. americana utilizando o Induteste SOS. Para avaliar a citotoxicidade da planta, depois de tratadas com diferentes concentrações do extrato, culturas de E. coli WP2s(λ foram diluνdas em tampão M9 e semeadas em placas LB. Para avaliar a genotoxicidade da planta, a cepa lisogênica WP2s(λ de E. coli foi tratada com diferentes concentrações do extrato. Em seguida, esta foi adicionada à cepa indicadora (RJF013 e ambas foram semeadas em placas em meio LB(1/2(malt(amp. Todas as culturas foram incubadas por 24

  5. Evaluación de Insecticidas para el Manejo de Monalonion velezangeli, Carvalho & Costa (Hemiptera: Miridae en Aguacate Evaluation of Insecticides for Monalonion velezangeli, Carvalho & Costa (Hemiptera: Miridae management in Avocado

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    Jimena Montilla Pérez

    2014-03-01

    Full Text Available Resumen. La chinche monalonion, coclicllo o chupanga(Monalonion velezangeli causa daños en brotes terminalesde aguacate, afectando el desarrollo del árbol; en frutos causa secamiento y, por lo tanto, rechazo en la comercialización. Aún no existen estrategias para el manejo de la plaga y los productores utilizan insecticidas sin previa evaluación de su efectividad. Se evaluaron insecticidas con un nuevo modo de acción sobre M. velezangeli bajo condiciones controladas. Se utilizó un diseño de bloques completos al azar, con once tratamientos y cuatrorepeticiones. Los tratamientos fueron los insecticidas deltametrina, λ-cihalotrina, imidacloprid, thiametoxam, spinosad, spiromesifen, diflubenzurón, diafentiurón, una mezcla de thiametoxam + λ-cihalotrina; y además, un testigo relativo (agua y un testigo absoluto (sin aplicación. La unidad experimental consistió de un arbusto de guayaba común con diez insectos confinados en una jaula de tela tul. Los tratamientos se aplicaron una vez con los insectos confinados en la jaula. Se identificó que deltametrina,λ-cihalotrina, thiametoxam, imidacloprid y la mezcla dethiametoxam más λ-cihalotrina causan mortalidad entre 85 y100% sobre M. velezangeli, 24 horas después de ser aplicados y 3 días después la mortalidad alcanza el 100%. Estos productos pueden ser incluidos en un programa de manejo integrado para M. velezangeli. El diafentiurón causa mortalidades entre 51-81%. No se evidenció efecto de diafentiurón, diflubenzurón y spiromesifen sobre la progenie de adultos expuestos, pero ninfas tratadas quealcanzaron el estado adulto, dieron origen a un menor número de ninfas; sin embargo, este efecto debe dilucidarse mejor. / Abstract. The bug monalonion, coclicllo o chupanga (Monalonion velezangeli generates damage in avocado end buds, affecting the tree development. When the attack is located on the fruit it causes its drying and therefore its rejection. There are no

  6. Potencial alelopático de plantas de acapu (Vouacapoua americana: efeitos sobre plantas daninhas de pastagens Allelopathic potential of "acapu" (Vouacapoua americana plants: effects on pasture weed

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    A.P.S. Souza Filho

    2000-01-01

    Full Text Available Extratos aquosos de folhas e cascas de plantas de acapu (Vouacapoua americana foram preparados nas concentrações de 0, 1, 2, 3 e 5% (v/v, visando identificar e caracterizar a atividade potencialmente alelopática dessa espécie. Analisaram-se os efeitos dos extratos sobre a germinação de sementes e o alongamento da raiz primária das plantas daninhas malícia (Mimosa pudica e malva (Urena lobata. Os bioensaios de germinação foram desenvolvidos em condições de 25 ºC e fotoperíodo de 12 horas. Para os bioensaios de alongamento da raiz primária, as condições estabelecidas foram de 25 ºC e fotoperíodo de 24 horas. Os resultados obtidos indicaram variações de respostas em função da fonte do extrato aquoso, do parâmetro analisado e da concentração do extrato. As reduções observadas tanto na germinação como no alongamento da raiz primária foram crescentes com o aumento da concentração do extrato, sendo os efeitos mais intensos observados na concentração de 5%. Independentemente da espécie receptora e do parâmetro analisado, o extrato preparado a partir das cascas do acapu evidenciou maior atividade alelopática inibitória. O alongamento da raiz primária foi o parâmetro mais sensível aos efeitos potencialmente alelopáticos do que a germinação das sementes. Comparativamente, cascas e folhas apresentaram diferenças em relação às classes de substâncias químicas. Nas cascas foram encontradas cumarinas que não estavam presentes nas folhas, as quais, por sua vez, apresentaram esteróides e triterpenóides, que não foram identificados nas cascas do acapu.Aqueous extracts from leaves and bark of "Acapu" plants were prepared in concentration levels of 0, 1, 2, 3 and 5% to identify and characterize potential allelopathic activity of such plants. The extract effects on seed germination and primary root elongation of pasture weeds, such as Mimosa pudica and Urena lobata, were analyzed. The germination bioassays were

  7. Mito e sociedade na literatura latino-americana: Neruda, Carpentier, Bolaño

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    Pedro Dolabela Chagas

    2013-09-01

    Full Text Available Resumo: Discussão sobre a problemática identitária na literatura latino-americana: a sua associação oitocentista à representação da natureza e da paisagem social; a sua longa duração entre os séculos XIX e XX; a sua politização pela dicotomia entre a América Latina e o Centro representado pela Europa e pelos EUA. A construção da identidade como produção de mitos: como construção de passados úteis, valoração positiva ou negativa do presente, projeção de futuros possíveis. A presença do mito em Canto geral, de Pablo Neruda; a sua recusa em 2666, de Roberto Bolaño; discussão sobre as razões desta recusa: a concentração no presente em detrimento da projeção do futuro. Localização de um tensionamento embrionário do mito identitário em Os passos perdidos, de Alejo Carpentier: discussão sobre os termos de orientação deste tensionamento e a diferença que ele produzia em relação à história anterior do romance latino-americano. Sugestão do fechamento recente da época histórica dominada pela problemática identitária na narrativa latino-americana.Palavras-chave: Pablo Neruda; Alejo Carpentier; Roberto Bolaño; romance latino-americano; mito e identidade. Abstract: The problematic of identity representation in Latin- American literature: its 19th Century association to the representation of nature and the social landscape; its long duration in the 19th and 20th Centuries; its politicization through the dichotomy between Latin America and a Center represented by Europe and the USA. Identity construction as a production of myths: as a construction of usable pasts, as a positive or negative evaluation of the present, as a projection of possible futures. The presence of myth in Pablo Neruda’s Canto general; its rejection in Roberto Bolaño’s 2666; the possible reasons of this rejection: a concentration in the present in detriment of the future. Location of an embrionary tensioning of the identity myths

  8. UJI STABILITAS FORMULA KRIM TABIR SURYA EKSTRAK UMBI BAWANG DAYAK (Eleutherine americana L. Merr.

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    Islamudin Ahmad

    2013-12-01

    Full Text Available Has conducted research on formulation and stability of sunscreen cream onion bulb extract dayak ( Eleutherine Americana L. Merr . . From the empirical data for the onions dayak has been used as an anti- cancer prevention and treatment by people of Borneo so it needs to be further investigated to obtain the data laboratory to support the empirical data . Several classes of secondary metabolites found in the bulbs of onions dayak alkaloids , glycosides , flavonoids , phenolics , steroids and tannins . The study was conducted as the basis for the formulation of sunscreen preparations aimed at the prevention of skin cancer . The study was conducted with bulb onions dayak extraction using Soxhlet method with solvent n - hexane , ethyl acetate , n - butanol , and ethanol as the active ingredient of the formula of making preparations . Tests conducted on formulations A and B with the concentration of garlic bulb extract dayak by 0.1% , consisting of physical stability test ( organoleptic , homogeneity and chemical ( pH and dosage in order to obtain a stable dosage formula . The test results obtained on the organoleptic test was no change in the observations made on each preparation before and after storage at room temperature on day - 7 , -14 , and -21 . Then the homogeneity test of the hedonic test results at 95% confidence level ( SNI 01-2346-2006 , concluded that the homogeneity of the preparation in all formulas produced in the category of smooth . Further testing on the pH stability and pH 5.5 to 8.0 to be produced that Formula A and Formula B to pH 7.0 to 7.7 with various concentrations of the extract , after 21 days of storage , inferred pH remains stable and safe dosage for used ( SNI 16-4399-1996 as formula sunscreen preparations made from onion bulb extract dayak . Key words : bulb onions dayak, sunscreen, formulas, test stability, skin cancer   ABSTRAK Telah dilakukan penelitian mengenai formulasi dan stabilitas krim tabir surya ekstrak

  9. Delineating and identifying long-term changes in the whooping crane (Grus americana) migration corridor

    Science.gov (United States)

    Pearse, Aaron T.; Rabbe, Matt; Juliusson, Lara M.; Bidwell, Mark T.; Craig-Moore, Lea; Brandt, David; Harrell, Wade C.

    2018-01-01

    Defining and identifying changes to seasonal ranges of migratory species is required for effective conservation. Historic sightings of migrating whooping cranes (Grus americana) have served as sole source of information to define a migration corridor in the Great Plains of North America (i.e., Canadian Prairies and United States Great Plains) for this endangered species. We updated this effort using past opportunistic sightings from 1942–2016 (n = 5,055) and more recent (2010–2016) location data from 58 telemetered birds (n = 4,423) to delineate migration corridors that included 50%, 75%, and 95% core areas. All migration corridors were well defined and relatively compact, with the 95% core corridor averaging 294 km wide, although it varied approximately ±40% in width from 170 km in central Texas to 407 km at the international border of the United States and Canada. Based on historic sightings and telemetry locations, we detected easterly movements in locations over time, primarily due to locations west of the median shifting east. This shift occurred from northern Oklahoma to central Saskatchewan at an average rate of 1.2 km/year (0.3–2.8 km/year). Associated with this directional shift was a decrease in distance of locations from the median in the same region averaging -0.7 km/year (-0.3–-1.3 km/year), suggesting a modest narrowing of the migration corridor. Changes in the corridor over the past 8 decades suggest that agencies and organizations interested in recovery of this species may need to modify where conservation and recovery actions occur. Whooping cranes showed apparent plasticity in their migratory behavior, which likely has been necessary for persistence of a wetland-dependent species migrating through the drought-prone Great Plains. Behavioral flexibility will be useful for whooping cranes to continue recovery in a future of uncertain climate and land use changes throughout their annual range.

  10. Coccidian Parasites and Conservation Implications for the Endangered Whooping Crane (Grus americana.

    Directory of Open Access Journals (Sweden)

    Miranda R Bertram

    Full Text Available While the population of endangered whooping cranes (Grus americana has grown from 15 individuals in 1941 to an estimated 304 birds today, the population growth is not sufficient to support a down-listing of the species to threatened status. The degree to which disease may be limiting the population growth of whooping cranes is unknown. One disease of potential concern is caused by two crane-associated Eimeria species: Eimeria gruis and E. reichenowi. Unlike most species of Eimeria, which are localized to the intestinal tract, these crane-associated species may multiply systemically and cause a potentially fatal disease. Using a non-invasive sampling approach, we assessed the prevalence and phenology of Eimeria oocysts in whooping crane fecal samples collected across two winter seasons (November 2012-April 2014 at the Aransas National Wildlife Refuge along the Texas Gulf coast. We also compared the ability of microscopy and PCR to detect Eimeria in fecal samples. Across both years, 26.5% (n = 328 of fecal samples were positive for Eimeria based on microscopy. Although the sensitivity of PCR for detecting Eimeria infections seemed to be less than that of microscopy in the first year of the study (8.9% vs. 29.3%, respectively, an improved DNA extraction protocol resulted in increased sensitivity of PCR relative to microscopy in the second year of the study (27.6% and 20.8%, respectively. The proportion of positive samples did not vary significantly between years or among sampling sites. The proportion of Eimeria positive fecal samples varied with date of collection, but there was no consistent pattern of parasite shedding between the two years. We demonstrate that non-invasive fecal collections combined with PCR and DNA sequencing techniques provides a useful tool for monitoring Eimeria infection in cranes. Understanding the epidemiology of coccidiosis is important for management efforts to increase population growth of the endangered whooping

  11. Aerobic Bacterial Community of American Cockroach Periplaneta americana,a Step toward Finding Suitable Paratransgenesis Candidates.

    Science.gov (United States)

    Akbari, Sanaz; Oshaghi, Mohammad Ali; Hashemi-Aghdam, Saedeh Sadat; Hajikhani, Sara; Oshaghi, Ghazaleh; Shirazi, Mohammad Hasan

    2015-06-01

    Cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. Identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. Here the bacterial microbiota of Periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate. Midgut of specimens were dissected and cultivated in different media. The bacterial isolates were then identified using the phenotypic and 16S-rRNA sequencing methods. The analytical profile index (API) kit showed presence of 11 bacterial species including: Escherichia coli, Shigella flexineri, Citrobacter freundii, E. vulneris, Enterobacter cloacae, Yersinia pseudotuberculosis, Y. intermedia, Leclericia adecarboxylata, Klebsiella oxytoca, K. planticola, and Rahnella aquatilis in the cockroach midguts. The first three species are potentially symbiotic whereas others are transient. The conventional plating method revealed presence of only four isolates of Salmonella, E. coli, and Proteus which in three cases mismatched with API and 16S-rRNA genotyping. The API correctly identified the four isolates as Shigella flexneri, Citrobacter freundii, and E. coli (n= 2). 16S-rRNA sequence analysis confirmed the API results; however the C. freundii sequence was identical with C. murliniae indicating lack of genetic variation in the gene between these two closely related species. A low number of potentially symbiotic bacteria were found in the American cockroach midguts. Among them Enterobacter cloacae is a potential candidate for paratransgenesis approach whereas other bacteria are pathogens and are not useful for the approach. Data analysis showed that identification levels increase from the conventional to API and to genotyping respectively.

  12. Aerobic Bacterial Community of American Cockroach Periplaneta americana,a Step toward Finding Suitable Paratransgenesis Candidates

    Directory of Open Access Journals (Sweden)

    Sanaz Akbari

    2015-10-01

    Full Text Available Background: Cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. Identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. Here the bacterial microbiota of Periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate.Methods: Midgut of specimens were dissected and cultivated in different media. The bacterial isolates were then identified using the phenotypic and 16S-rRNA sequencing methods.Results: The analytical profile index (API kit showed presence of 11 bacterial species including: Escherichia coli, Shigella flexineri, Citrobacter freundii, E. vulneris, Enterobacter cloacae, Yersinia pseudotuberculosis, Y.intermedia, Leclericia adecarboxylata, Klebsiella oxytoca, K. planticola, and Rahnella aquatilis in the cockroach midguts. The first three species are potentially symbiotic whereas others are transient. The conventional platingmethod revealed presence of only four isolates of Salmonella, E. coli, and Proteus which in three cases mismatched with API and 16S-rRNA genotyping. The API correctly identified the four isolates as Shigella flexneri, Citrobacter freundii, and E. coli (n= 2. 16S-rRNA sequence analysis confirmed the API results; however the C. freundii sequencewas identical with C. murliniae indicating lack of genetic variation in the gene between these two closely related species.Conclusion: A low number of potentially symbiotic bacteria were found in the American cockroach midguts. Among them Enterobacter cloacae is a potential candidate for paratransgenesis approach whereas other bacteria are pathogens and are not useful for the approach. Data analysis showed that identification levels increase from the conventional to API and to genotyping respectively.

  13. Antioxidant and Hypolipidemic Activity of the Hydroethanolic Extract of Curatella americana L. Leaves.

    Science.gov (United States)

    Lopes, Rafael Henrique Oliveira; Macorini, Luis Fernando Benitez; Antunes, Katia Ávila; Espindola, Priscilla Pereira de Toledo; Alfredo, Tamaeh Monteiro; da Rocha, Paola Dos Santos; Pereira, Zefa Valdivina; Dos Santos, Edson Lucas; de Picoli Souza, Kely

    2016-01-01

    High levels of reactive oxygen species in the body and hyperlipidemia are key factors for the development of cardiovascular diseases such as atherosclerosis. The present study investigated the antioxidant and hypolipidemic activity of hydroethanolic extract of Curatella americana L. leaves (ExC). The antioxidant activity of ExC was assessed by 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging capacity and protection against hemolysis induced by 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), followed by quantification of malondialdehyde (MDA). Wistar rats with hyperlipidemia induced by high-fructose diet (60%) were treated for 60 days with water, simvastatin (30 mg·Kg(-1)), ciprofibrate (2 mg·Kg(-1)), and ExC (200 mg·Kg(-1)). ExC revealed IC50 of 6.0 ± 0.5 μg·mL(-1), an intermediary value among positive controls used in the assay of DPPH scavenging capacity. At all concentrations (50 to 125 μg·mL(-1)) and times (60 to 240 min) evaluated, ExC protected erythrocytes against AAPH-induced hemolysis, which was confirmed by lower MDA levels. In vivo tests showed a reduction of 34 and 45%, respectively, in serum concentration of cholesterol and triglycerides in hyperlipidemic rats treated with ExC, a similar effect compared to the reference drugs, simvastatin and ciprofibrate, respectively. Together, the results showed the antioxidant activity of ExC and its ability to improve the serum lipid profile in hyperlipidemic rats.

  14. Antioxidant and Hypolipidemic Activity of the Hydroethanolic Extract of Curatella americana L. Leaves

    Directory of Open Access Journals (Sweden)

    Rafael Henrique Oliveira Lopes

    2016-01-01

    Full Text Available High levels of reactive oxygen species in the body and hyperlipidemia are key factors for the development of cardiovascular diseases such as atherosclerosis. The present study investigated the antioxidant and hypolipidemic activity of hydroethanolic extract of Curatella americana L. leaves (ExC. The antioxidant activity of ExC was assessed by 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH scavenging capacity and protection against hemolysis induced by 2,2′-azobis(2-amidinopropane dihydrochloride (AAPH, followed by quantification of malondialdehyde (MDA. Wistar rats with hyperlipidemia induced by high-fructose diet (60% were treated for 60 days with water, simvastatin (30 mg·Kg−1, ciprofibrate (2 mg·Kg−1, and ExC (200 mg·Kg−1. ExC revealed IC50 of 6.0±0.5 μg·mL−1, an intermediary value among positive controls used in the assay of DPPH scavenging capacity. At all concentrations (50 to 125 μg·mL−1 and times (60 to 240 min evaluated, ExC protected erythrocytes against AAPH-induced hemolysis, which was confirmed by lower MDA levels. In vivo tests showed a reduction of 34 and 45%, respectively, in serum concentration of cholesterol and triglycerides in hyperlipidemic rats treated with ExC, a similar effect compared to the reference drugs, simvastatin and ciprofibrate, respectively. Together, the results showed the antioxidant activity of ExC and its ability to improve the serum lipid profile in hyperlipidemic rats.

  15. Experimental study of convective coefficient of mass transfer of avocado (Persia americana Mill.)

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Suerda Bezerra; Luiz, Marcia Ramos; Amorim, Joselma Araujo de; Gusmao, Rennam Pereira de; Gurgel, Jose Mauricio [Universidade Federal da Paraiba (LES/UFPB), Joao Pessoa, PB (Brazil). Lab. de Energia Solar

    2010-07-01

    Most of all energy consumed worldwide comes from fossil fuels derived from petroleum. With the petroleum crisis in the 70 were sought new energy sources, among them renewable. One such source is biodiesel energy, organic matter originated from animal and/or vegetable. Among the various plant species is the avocado (Persia americana Mill.) showing great potential in the production of petroleum extracted from the pulp and the alcohol removed from the seed. The main obstacle for obtaining the petroleum is the high humidity found in the pulp, being necessary to the drying process, which involves the transfer of heat and mass. The aim of this study was to use the mathematical model represented by Newton's Law of Cooling to simulate the mass transfer on the surface of the avocado pulp during the drying process. The equation of the mathematical model was solved numerically and the method of least squares was identified convective coefficient of Mass Transfer. The dryer used in the experimental process was operated with air flow in the vertical, air flow average fixed 3m/s and temperatures of 50, 60 and 70 deg C. The scheme of the dryer used in the research is composed of the following equipment: centrifugal fan, which drives the air-drying; valve, which allows control of airflow; electrical resistance, used for heating air; the drying chamber, where enables measurement of temperature and relative humidity; support for smaller trays; trays smaller, where the samples of the pulp of the avocado are placed; exit of the air of drying for the environment. The result presented shows the ratio of moisture content as a function of temperature over time, where it is possible to also observe that how much bigger the temperature of drying, greater will be the convective coefficient of mass transfer of the avocado. (author)

  16. Injúrias causadas por mosca-das-frutas sul-americana em cultivares de videira

    Directory of Open Access Journals (Sweden)

    Marcelo Zart

    2011-01-01

    Full Text Available A mosca-das-frutas sul-americana, Anastrepha fraterculus (Wiedemann (Diptera: Tephritidae, é uma das principais pragas da fruticultura no Brasil. O objetivo deste estudo foi identificar a suscetibilidade quanto à queda e deformação de bagas de diferentes cultivares/espécies de uva pela injúria causada pela oviposição de A. fraterculus. O trabalho foi realizado em vinhedos das cultivares 'Cabernet Sauvignon' (Vitis vinifera, 'Moscato Embrapa' (75% de V. vinifera e 'Isabel' (Vitis labrusca, localizados em Bento Gonçalves (RS, na safra 2006/2007. Dois casais de A. fraterculus foram confinados por cacho, em gaiolas de tecido voile, durante os estádios fenológicos de grão ervilha, início da compactação, início da maturação e maturação plena dos cachos. Queda significativa de bagas devido ao ataque de A. fraterculus foi registrada na cultivar 'Cabernet Sauvignon' quando a infestação foi realizada nos estádios fenológicos de grão ervilha e início da compactação de cacho; estádios de grão ervilha em 'Moscato Embrapa'; e nos estádios de grão ervilha, início da compactação e início da maturação do cacho na cultivar 'Isabel'. Foi registrada deformação de bagas quando as infestações foram realizadas nos estádios de grão ervilha e início da compactação de cacho em 'Cabernet Sauvignon', 'Moscato Embrapa' e 'Isabel'. O desenvolvimento larval até a fase de pupa foi observado somente na cultivar 'Moscato Embrapa'.

  17. Leishmaniasis tegumentaria americana: encuesta epidemiológica en una comunidad indígena

    Directory of Open Access Journals (Sweden)

    Martha Wolff

    1988-01-01

    Full Text Available Se realizó un estudio epidemiológico sobre Leishmaniasis tegumentarla americana (LTA en 809 personas del Resguardo Indígena de San Andrés de Sotavento, Departamento de Córdoba, foco endémico tanto de Leishmaniasis cutánea como de Leishmaniasis visceral. La distribución por sexo de la población encuestada fue de 355 (43,9% hombres y 454 (56, 1% mujeres; sus índices alérgicos fueron de 36,9% y 40,5% respectivamente. La positividad de la Intradermorreacción de Montenegro ascendió en personas de ambos sexos, a medida que aumentó la edad. El índice parasitario fue de 0.37%. La especie de Lutzomyia más frecuente en el intra y peridomicilio fue la Lu. evansi que mostró comportamiento antropofílico. Se encontró una alta exposición de la población general al vector dada la facilidad de penetración de los flebotomineos alintradomicilio, por el tipo de construcción de las viviendas; además, al no existir servicios sanitarios, acueductos ni alcantarillados Intradomiciliarios, todo el núcleo familiar debe desplazarse al peridomicilio para la disposición de excretas y en busca del agua. Otros hallazgos fueron el gran desconocimiento sobre la etiología y tratamiento de la Leishmaniasis y las pésimas condiciones sociales y económicas de la región.

  18. Symbiotic essential amino acids provisioning in the American cockroach, Periplaneta americana (Linnaeus) under various dietary conditions.

    Science.gov (United States)

    Ayayee, Paul A; Larsen, Thomas; Sabree, Zakee

    2016-01-01

    Insect gut microbes have been shown to provide nutrients such as essential amino acids (EAAs) to their hosts. How this symbiotic nutrient provisioning tracks with the host's demand is not well understood. In this study, we investigated microbial essential amino acid (EAA) provisioning in omnivorous American cockroaches (Periplaneta americana), fed low-quality (LQD) and comparatively higher-quality dog food (DF) diets using carbon stable isotope ratios of EAAs (δ (13)CEAA). We assessed non-dietary EAA input, quantified as isotopic offsets (Δ(13)C) between cockroach (δ (13)CCockroach EAA) and dietary (δ (13)CDietary EAA) EAAs, and subsequently determined biosynthetic origins of non-dietary EAAs in cockroaches using (13)C-fingerprinting with dietary and representative bacterial and fungal δ (13)CEAA. Investigation of biosynthetic origins of de novo non-dietary EAAs indicated bacterial origins of EAA in cockroach appendage samples, and a mixture of fungal and bacterial EAA origins in gut filtrate samples for both LQD and DF-fed groups. We attribute the bacteria-derived EAAs in cockroach appendages to provisioning by the fat body residing obligate endosymbiont, Blattabacterium and gut-residing bacteria. The mixed signatures of gut filtrate samples are attributed to the presence of unassimilated dietary, as well as gut microbial (bacterial and fungal) EAAs. This study highlights the potential impacts of dietary quality on symbiotic EAA provisioning and the need for further studies investigating the interplay between host EAA demands, host dietary quality and symbiotic EAA provisioning in response to dietary sufficiency or deficiency.

  19. Restrição hídrica em sementes de Jenipapo (Genipa americana L.

    Directory of Open Access Journals (Sweden)

    Allívia Rouse Ferreira dos Santos

    2011-04-01

    Full Text Available O jenipapo, originário da América Latina, pertence à família Rubiaceae e apresenta valor madeireiro e alimentício, sendo explorado sem estratégias de conservação. O objetivo deste trabalho foi avaliar o efeito da restrição hídrica, por meio de duas metodologias, na germinação de sementes de Genipa americana L. A primeira foi realizada com sementes imersas em soluções de polietilenoglicol (PEG 6000 por 18 dias a 0,0; -0,1; -0,3 e -0,4 MPa à 20ºC, simulando condições de alagamento, e após este período as sementes foram colocadas para germinar em placas de Petri com folhas de papel tipo mata-borrão umedecidas em água. A segunda utilizou os mesmos potenciais osmóticos umedecendo-se os papéis tipo mata-borrão, para simular a restrição hídrica. O delineamento utilizado foi o inteiramente casualizado com 4 repetições de 25 sementes. Para as sementes imersas em soluções de PEG, o potencial -0,3MPa permite condicionamento osmótico, enquanto à -0,4MPa interfere no vigor. Para as sementes embebidas em papel, ocorre redução da germinação em função do tempo, até não se observar eventos para potenciais a partir de -0,3 MPa.

  20. A produção da "cidade latino-americana" The production of "the Latin American city"

    Directory of Open Access Journals (Sweden)

    Adrián Gorelik

    2005-06-01

    Full Text Available Este trabalho propõe uma análise histórica da categoria "cidade latino-americana" como construção cultural. O argumento central é que, durante o período compreendido entre o segundo pós-guerra e a década de 1970, a idéia de "cidade latino-americana" funcionou como uma categoria do pensamento social, como uma figura do imaginário intelectual e político em amplas regiões do continente. Este texto procura reconstruir os principais itinerários conceituais e ideológicos da categoria, suas funções políticas e institucionais na conjuntura específica da região. Esse modo de entender a categoria "cidade latino-americana" em sua configuração histórica específica, sugere o autor, pode oferecer uma via produtiva para os estudos culturais latino-americanos.This paper suggests a historical analysis of the category "Latin American city" as a cultural construct. The central argument is that, during the period between the post-war and the 1970's, the idea of a Latin American city became a category of social thought, as part of the intellectual and political imagination in several regions of the continent. The text tries to rebuild the main conceptual and ideological turns, their political and institutional functions within the specific regional circumstances. The article suggests that this approach to the "Latin American city" category in its precise historical configuration may be a productive path for Latin-American cultural studies.

  1. Efecto antiinflamatorio de la Ephedra americana HYB sobre la encía de cobayos en procedimiento quirúrgico

    OpenAIRE

    Arriola Guillén, Luis; Sacsaquispe-Contreras, Sonia

    2014-01-01

    Objetivo: Evaluar el efecto antiinflamatorio de la Ephedra americana HyB en cirugía gingival en cobayos. Materiales y Métodos: Estudio experimental en 32 cobayos: 16 cobayos a los que se les aplicó el extracto etanólico de la planta (grupo experimental) y 16 el grupo control. Se les realizó incisión tipo media luna en la encía inferior, con desprendimient o del colgajo y se suturó; luego se obtuvieron biopsias excisionales a las 48 horas y a los 7 días para evaluación histopatológica del proc...

  2. Variation in manuka oil lure efficacy for capturing Xyleborus glabratus (Coleoptera: Curculionidae: Scholytinae), and Cubeb oil as an alternative attractant

    Science.gov (United States)

    James Hanula; Brian Sullivan; David Wakarchuk

    2013-01-01

    Redbay ambrosia beetle, Xyleborus glabratus Eichoff, is an exotic species to North America vectoring a deadly vascular wilt disease of redbay [Persea borbonia (L.) Spreng], swampbay [P. palustris (Raf.) Sarg.], avocado (P. americana Mill.), and sassafras [Sassafras albidum (...

  3. Author Details

    African Journals Online (AJOL)

    Persea americana) seeds and fluted pumpkin (Telfairia occidentalis) leaves coadministered with anti-tuberculosis drugs on liver enzymes of albino rats. Abstract PDF. ISSN: 2006-6996. AJOL African Journals Online. HOW TO USE AJOL.

  4. Author Details

    African Journals Online (AJOL)

    oxidant effects of Persea americana fruit extract studied in rats introduced into streptzotocin-induced hyperglycaemic state. Abstract PDF. ISSN: 0189-6016. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians ...

  5. Novel inhibitory activity of the Staphylococcus aureus NorA efflux pump by a kaempferol rhamnoside isolated from Persea lingue Nees.

    Science.gov (United States)

    Holler, Jes Gitz; Christensen, S Brøgger; Slotved, Hans-Christian; Rasmussen, Hasse B; Gúzman, Alfonso; Olsen, Carl-Erik; Petersen, Bent; Mølgaard, Per

    2012-05-01

    To isolate a plant-derived compound with efflux inhibitory activity towards the NorA transporter of Staphylococcus aureus. Bioassay-guided isolation was used, with inhibition of ethidium bromide efflux via NorA as a guide. Characterization of activity was carried out using MIC determination and potentiation studies of a fluoroquinolone antibiotic in combination with the isolated compound. Everted membrane vesicles of Escherichia coli cells enriched with NorA were prepared to study efflux inhibitory activity in an isolated manner. The ethanolic extract of Persea lingue was subjected to bioassay-guided fractionation and led to the isolation of the known compound kaempferol-3-O-α-L-(2,4-bis-E-p-coumaroyl)rhamnoside (compound 1). Evaluation of the dose-response relationship of compound 1 showed that ethidium bromide efflux was inhibited, with an IC(50) value of 2 μM. The positive control, reserpine, was found to have an IC(50) value of 9 μM. Compound 1 also inhibited NorA in enriched everted membrane vesicles of E. coli. Potentiation studies revealed that compound 1 at 1.56 mg/L synergistically increased the antimicrobial activity of ciprofloxacin 8-fold against a NorA overexpresser, and the synergistic activity was exerted at a fourth of the concentration necessary for reserpine. Compound 1 was not found to exert a synergistic effect on ciprofloxacin against a norA deletion mutant. The 2,3-coumaroyl isomer of compound 1 has been shown previously not to cause acute toxicity in mice at 20 mg/kg/day. Our results show that compound 1 acts through inhibition of the NorA efflux pump. Combination of compound 1 with subinhibitory concentrations of ciprofloxacin renders a wild-type more susceptible and a NorA overexpresser S. aureus susceptible.

  6. Flavonoid glycosides from Persea caerulea. Unraveling their interactions with SDS-micelles through matrix-assisted DOSY, PGSE, mass spectrometry, and NOESY.

    Science.gov (United States)

    Álvarez, Juan M; Raya-Barón, Álvaro; Nieto, Pedro M; Cuca, Luis E; Carrasco-Pancorbo, Alegría; Fernández-Gutiérrez, Alberto; Fernández, Ignacio

    2016-04-13

    Two flavonoid glycosides derived from rhamnopyranoside (1) and arabinofuranoside (2) have been isolated from leaves of Persea caerulea for the first time. The structures of 1 and 2 have been established by 1 H NMR, 13 C NMR, and IR spectroscopy, together with LC-ESI-TOF and LC-ESI-IT MS spectrometry. From the MS and MS/MS data, the molecular weights of the intact molecules as well as those of quercetin and kaempferol together with their sugar moieties were deduced. The NMR data provided information on the identity of the compounds, as well as the α and β configurations and the position of the glycosides on quercetin and kaempferol. We have also explored the application of sodium dodecyl sulfate (SDS) normal micelles in binary aqueous solution, at a range of concentrations, to the diffusion resolution of these two glycosides, by the application of matrix-assisted diffusion ordered spectroscopy (DOSY) and pulse field gradient spin echo (PGSE) methodologies, showing that SDS micelles offer a significant resolution which can, in part, be rationalized in terms of differing degrees of hydrophobicity, amphiphilicity, and steric effects. In addition, intra-residue and inter-residue proton-proton distances using nuclear Overhauser effect build-up curves were used to elucidate the conformational preferences of these two flavonoid glycosides when interacting with the micelles. By the combination of both diffusion and nuclear Overhauser spectroscopy techniques, the average location site of kaempferol and quercetin glycosides has been postulated, with the former exhibiting a clear insertion into the interior of the SDS-micelle, whereas the latter is placed closer to the surface. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  7. First report of laurel wilt, caused by Raffaelea lauricola , on Sassafras ( Sassafras albidum ) in Arkansas

    Science.gov (United States)

    Rabiu Olatinwo; C. Barton; Stephen Fraedrich; W. Johnson; J. Hwang

    2016-01-01

    Laurel wilt, caused by Raffaelea lauricola T.C. Harrin., Aghayeva & Fraedrich, is a lethal disease that affects members of the laurel family (Lauraceae) including avocado (Persea americana Mill), redbay (Persea borbonia [L.] Spreng., Nees), and sassafras (Sassafras albidum [Nutt.]). The fungus is a symbiont of an exotic ambrosia beetle (Xyleborus glabratus...

  8. PeaTAR1B: Characterization of a Second Type 1 Tyramine Receptor of the American Cockroach, Periplaneta americana.

    Science.gov (United States)

    Blenau, Wolfgang; Balfanz, Sabine; Baumann, Arnd

    2017-10-30

    The catecholamines norepinephrine and epinephrine regulate important physiological functions in vertebrates. In insects; these neuroactive substances are functionally replaced by the phenolamines octopamine and tyramine. Phenolamines activate specific guanine nucleotide-binding (G) protein-coupled receptors (GPCRs). Type 1 tyramine receptors are better activated by tyramine than by octopamine. In contrast; type 2 tyramine receptors are almost exclusively activated by tyramine. Functionally; activation of type 1 tyramine receptors leads to a decrease in the intracellular concentration of cAMP ([cAMP] i ) whereas type 2 tyramine receptors can mediate Ca 2+ signals or both Ca 2+ signals and effects on [cAMP] i . Here; we report that the American cockroach ( Periplaneta americana ) expresses a second type 1 tyramine receptor (PeaTAR1B) in addition to PeaTAR1A (previously called PeaTYR1). When heterologously expressed in flpTM cells; activation of PeaTAR1B by tyramine leads to a concentration-dependent decrease in [cAMP] i . Its activity can be blocked by a series of established antagonists. The functional characterization of two type 1 tyramine receptors from P. americana ; PeaTAR1A and PeaTAR1B; which respond to tyramine by changing cAMP levels; is a major step towards understanding the actions of tyramine in cockroach physiology and behavior; particularly in comparison to the effects of octopamine.

  9. PeaTAR1B: Characterization of a Second Type 1 Tyramine Receptor of the American Cockroach, Periplaneta americana

    Directory of Open Access Journals (Sweden)

    Wolfgang Blenau

    2017-10-01

    Full Text Available The catecholamines norepinephrine and epinephrine regulate important physiological functions in vertebrates. In insects; these neuroactive substances are functionally replaced by the phenolamines octopamine and tyramine. Phenolamines activate specific guanine nucleotide-binding (G protein-coupled receptors (GPCRs. Type 1 tyramine receptors are better activated by tyramine than by octopamine. In contrast; type 2 tyramine receptors are almost exclusively activated by tyramine. Functionally; activation of type 1 tyramine receptors leads to a decrease in the intracellular concentration of cAMP ([cAMP]i whereas type 2 tyramine receptors can mediate Ca2+ signals or both Ca2+ signals and effects on [cAMP]i. Here; we report that the American cockroach (Periplaneta americana expresses a second type 1 tyramine receptor (PeaTAR1B in addition to PeaTAR1A (previously called PeaTYR1. When heterologously expressed in flpTM cells; activation of PeaTAR1B by tyramine leads to a concentration-dependent decrease in [cAMP]i. Its activity can be blocked by a series of established antagonists. The functional characterization of two type 1 tyramine receptors from P. americana; PeaTAR1A and PeaTAR1B; which respond to tyramine by changing cAMP levels; is a major step towards understanding the actions of tyramine in cockroach physiology and behavior; particularly in comparison to the effects of octopamine.

  10. Mechanisms of Egg Yolk Formation and Implications on Early Life History of White Perch (Morone americana.

    Directory of Open Access Journals (Sweden)

    Justin Schilling

    Full Text Available The three white perch (Morone americana vitellogenins (VtgAa, VtgAb, VtgC were quantified accurately and precisely in the liver, plasma, and ovary during pre-, early-, mid-, and post-vitellogenic oocyte growth using protein cleavage-isotope dilution mass spectrometry (PC-IDMS. Western blotting generally mirrored the PC-IDMS results. By PC-IDMS, VtgC was quantifiable in pre-vitellogenic ovary tissues and VtgAb was quantifiable in pre-vitellogenic liver tissues however, neither protein was detected by western blotting in these respective tissues at this time point. Immunohistochemistry indicated that VtgC was present within pre-vitellogenic oocytes and localized to lipid droplets within vitellogenic oocytes. Affinity purification coupled to tandem mass spectrometry using highly purified VtgC as a bait protein revealed a single specific interacting protein (Y-box binding protein 2a-like [Ybx2a-like] that eluted with suramin buffer and confirmed that VtgC does not bind the ovary vitellogenin receptors (LR8 and Lrp13. Western blotting for LR8 and Lrp13 showed that both receptors were expressed during vitellogenesis with LR8 and Lrp13 expression highest in early- and mid-vitellogenesis, respectively. The VtgAa within the ovary peaked during post-vitellogenesis, while VtgAb peaked during early-vitellogenesis in both white perch and the closely related striped bass (M. saxatilis. The VtgC was steadily accumulated by oocytes beginning during pre-vitellogenesis and continued until post-vitellogenesis and its composition varies widely between striped bass and white perch. In striped bass, the VtgC accounted for 26% of the vitellogenin-derived egg yolk, however in the white perch it comprised only 4%. Striped bass larvae have an extended developmental window and these larvae have yolk stores that may enable them to survive in the absence of food for twice as long as white perch after hatch. Thus, the VtgC may play an integral role in providing nutrients to

  11. The rhizome of Reclinomonas americana, Homo sapiens, Pediculus humanus and Saccharomyces cerevisiae mitochondria

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    Raoult Didier

    2011-10-01

    Full Text Available Abstract Background Mitochondria are thought to have evolved from eubacteria-like endosymbionts; however, the origin of the mitochondrion remains a subject of debate. In this study, we investigated the phenomenon of chimerism in mitochondria to shed light on the origin of these organelles by determining which species played a role in their formation. We used the mitochondria of four distinct organisms, Reclinomonas americana, Homo sapiens, Saccharomyces cerevisiae and multichromosome Pediculus humanus, and attempted to identify the origin of each mitochondrial gene. Results Our results suggest that the origin of mitochondrial genes is not limited to the Rickettsiales and that the creation of these genes did not occur in a single event, but through multiple successive events. Some of these events are very old and were followed by events that are more recent and occurred through the addition of elements originating from current species. The points in time that the elements were added and the parental species of each gene in the mitochondrial genome are different to the individual species. These data constitute strong evidence that mitochondria do not have a single common ancestor but likely have numerous ancestors, including proto-Rickettsiales, proto-Rhizobiales and proto-Alphaproteobacteria, as well as current alphaproteobacterial species. The analysis of the multichromosome P. humanus mitochondrion supports this mechanism. Conclusions The most plausible scenario of the origin of the mitochondrion is that ancestors of Rickettsiales and Rhizobiales merged in a proto-eukaryotic cell approximately one billion years ago. The fusion of the Rickettsiales and Rhizobiales cells was followed by gene loss, genomic rearrangements and the addition of alphaproteobacterial elements through ancient and more recent recombination events. Each gene of each of the four studied mitochondria has a different origin, while in some cases, multichromosomes may allow for

  12. Enterobactérias isoladas de baratas (Periplaneta americana capturadas em um hospital brasileiro

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    Marinésia A. Prado

    2002-02-01

    Full Text Available Objetivo. Isolar e identificar microrganismos em baratas capturadas em um hospital público e determinar o seu perfil de suscetibilidade antimicrobiana. Métodos. As baratas foram capturadas nos períodos matutino e noturno, colocadas em frascos desinfetados com álcool a 70%, transferidas para um frasco estéril e levadas ao laboratório. Consideraram-se as baratas íntegras e vivas, as quais foram colocadas em solução salina estéril (0,8% e homogeneizadas. Essa solução foi semeada nos meios de cultura ágar MacConkey, caldo nutriente, infusão de cérebro e coração (ágar BHI, ágar Sabouraud e ágar manitol. As culturas foram examinadas em um estereomicroscópio para a contagem das unidades formadoras de colônias. Para a determinação do perfil de suscetibilidade antimicrobiana utilizou-se o teste de difusão de disco. Resultados. Detectou-se prevalência de 56% de enterobactérias e de 18% de estafilococos coagulase negativos. Identificaram-se 15 espécies de enterobactérias. As mais freqüentes foram Klebsiella pneumoniae (17%; Enterobacter aerogenes (14%; Serratia marcescens (13%; Hafnia alvei (12%; Enterobacter gergoviae e Enterobacter cloacae (9%; e Serratia spp. (6%. Tanto as enterobactérias quanto os estafilococos coagulase negativos apresentaram uma resistência significativa aos antimicrobianos, inclusive à oxacilina. Conclusões. A prevalência de bactérias enteropatogênicas e de estafilococos coagulase negativos isolados de baratas Periplaneta americana no hospital estudado demonstra a fragilidade das condutas adotadas tanto para o controle de vetores quanto para o uso dos antimicrobianos. Os resultados demonstram a necessidade da implementação de um programa efetivo de saneamento ambiental e do uso racional dos antimicrobianos dentro das instituições de saúde.

  13. Enterobactérias isoladas de baratas (Periplaneta americana capturadas em um hospital brasileiro

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    Prado Marinésia A.

    2002-01-01

    Full Text Available Objetivo. Isolar e identificar microrganismos em baratas capturadas em um hospital público e determinar o seu perfil de suscetibilidade antimicrobiana. Métodos. As baratas foram capturadas nos períodos matutino e noturno, colocadas em frascos desinfetados com álcool a 70%, transferidas para um frasco estéril e levadas ao laboratório. Consideraram-se as baratas íntegras e vivas, as quais foram colocadas em solução salina estéril (0,8% e homogeneizadas. Essa solução foi semeada nos meios de cultura ágar MacConkey, caldo nutriente, infusão de cérebro e coração (ágar BHI, ágar Sabouraud e ágar manitol. As culturas foram examinadas em um estereomicroscópio para a contagem das unidades formadoras de colônias. Para a determinação do perfil de suscetibilidade antimicrobiana utilizou-se o teste de difusão de disco. Resultados. Detectou-se prevalência de 56% de enterobactérias e de 18% de estafilococos coagulase negativos. Identificaram-se 15 espécies de enterobactérias. As mais freqüentes foram Klebsiella pneumoniae (17%; Enterobacter aerogenes (14%; Serratia marcescens (13%; Hafnia alvei (12%; Enterobacter gergoviae e Enterobacter cloacae (9%; e Serratia spp. (6%. Tanto as enterobactérias quanto os estafilococos coagulase negativos apresentaram uma resistência significativa aos antimicrobianos, inclusive à oxacilina. Conclusões. A prevalência de bactérias enteropatogênicas e de estafilococos coagulase negativos isolados de baratas Periplaneta americana no hospital estudado demonstra a fragilidade das condutas adotadas tanto para o controle de vetores quanto para o uso dos antimicrobianos. Os resultados demonstram a necessidade da implementação de um programa efetivo de saneamento ambiental e do uso racional dos antimicrobianos dentro das instituições de saúde.

  14. Entre Debates e Embates: uma reflexão sobre epistemologias feministas latino-americanas

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    Renata Araújo Matos

    2017-04-01

    Full Text Available Resumo O presente texto objetiva discutir proposições elaboradas por parte de pensadoras feministas latino-americanas, pautando sua contribuição para os estudos teóricos que buscam compreender a construção dos padrões coloniais que ainda persistem na análise social da região. Entende-se que é pertinente fazer referência aos debates e confrontos internos aos movimentos e ao pensamento feminista para, dessa forma, evitar o caminho simplificador de não-reconhecimento, sem exceções, das contribuições do pensamento social já institucionalizado. Palavras-chaves: mulheres; feminismos latino-americanos; epistemologias feministas.   Between Debates and Blocks: a Reflection on Latin American Feminists Epistemologies Abstract The present article aims to discuss Latin American thinkers propositions and how their contributions impact on the regional decolonial studies. It is understood that it is pertinent to refer to debates and internal confrontations with feminist movements and thinking. In this way, one can avoid the simplistic path of non-recognition, without exceptions, of the contributions of already institutionalized social thought. Keywords: Women, Latin American Feminists, Feminists Epistemologies   Entre Debates y Embates: una reflexión sobre epistemologías feministas latinoamericanas Resumen El presente texto tiene como objetivo discutir proposiciones elaboradas por parte de pensadoras feministas latinoamericanas, pautando su contribución para los estudios teóricos que buscan comprender la construcción de los estándares coloniales que aún persisten en el análisis social de la región. Se considera pertinente hacer referencia a los debates y confrontaciones internos a los movimientos y al pensamiento feminista para, de esa forma, evitar el camino simplificador de no-reconocimiento, sin excepciones, de las contribuciones del pensamiento social ya institucionalizado.     Palabras-clave: mujeres; feminismos

  15. Desidratação osmótica do jenipapo (Genipa americana L.

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    Andrade Samara Alvachian Cardoso

    2003-01-01

    Full Text Available O processo de desidratação osmótica foi aplicado ao Jenipapo (Genipa americana L., para reduzir as perdas pós-colheita, diversificar o seu aproveitamento industrial e obter um produto com boas características organolépticas e boa estabilidade microbiológica. Os frutos foram adquiridos na feira livre de Afogados, na região metropolitana do Recife, nas condições em que são comercializados, subdivididos em cubos e submetidos a diversas condições de processamento, segundo planejamento fatorial 2³ de modo a determinar a influência das variáveis independentes: temperatura, tipo de açúcar e concentração do agente osmótico sobre a perda de umidade, ganho de açúcar e redução de peso. O processo foi complementado por secagem em estufa, com ventilação forçada a 60ºC±5ºC, por 3h±10', seguida do acondicionamento do produto em embalagem de polietileno e armazenamento à temperatura ambiente (28ºC±3ºC, por 90 dias. Os resultados demonstraram que: os produtos obtidos apresentaram significativa incorporação de sólidos; nenhuma das respostas foi influenciada pelo tipo de açúcar empregado, diferentemente da concentração do agente osmótico que além de refletir sobre todas elas, interagiu com a temperatura influindo sobre a perda de umidade; durante o armazenamento, os produtos apresentaram-se microbiologicamente estáveis, sem diferenças significativas, no que concerne às características organolépticas, embora os escores demonstrem uma preferência pelo produto obtido a partir de 60ºBrix, 60ºC e mistura de açúcar demerara e cristal. A elevada incorporação de sólidos ocorrida aponta para necessidade de proceder à otimização do produto com vista a sua redução.

  16. Evaluation of Cruzia americana, Turgida turgida, and Didelphostrongylus hayesi infection in the Virginia opossum (Didelphis virginiana) and risk factors along the California coast.

    Science.gov (United States)

    Nichelason, Amy E; Rejmanek, Dan; Dabritz, Haydee A; Melli, Ann C; Miller, Melissa; Conrad, Patricia A

    2008-10-01

    Three nematodes, Turgida turgida, Cruzia americana, and Didelphostrongylus hayesi, have been documented to cause morbidity and mortality in the Virginia opossum (Didelphis virginiana). The present study was designed to determine the frequency of infection of these nematodes in opossums at 2 study sites in California and to determine if there are risk factors associated with shedding of eggs or larvae in the feces. Turgida turgida and C. americana adults were found in 84.4% (stomach; n = 45) and 62.5% (intestinal wash and feces; n = 16) of sampled opossums. Eggs were present in opossum feces (n = 105) less frequently (40% T. turgida and 35.2% C. americana). Didelphostrongylus hayesi larvae were found in 79.0% of opossum feces examined (n = 105). Adult age and wet season (December through April) were significant predictive factors for the presence of T. turgida eggs, whereas the dry season (May through November) was significantly associated with the presence of C. americana eggs in feces. Adult opossums were more likely to have eggs and larvae from all 3 nematodes in the feces.

  17. PHYSIOLOGICAL AND FOLIAR INJURY RESPONSES OF PRUNUS SEROTINA, FRAXINUS AMERICANA, AND ACER RUBRUM SEEDLINGS TO VARYING SOIL MOISTURE AND OZONE. (R825244)

    Science.gov (United States)

    Sixteen black cherry (Prunus serotina, Ehrh.), 10 white ash (Fraxinus americana, L.) and 10 red maple (Acer rubrum, L.) 1-year old seedlings were planted per plot in 1997 on a former nursery bed within 12 open-top chambers and six open plots. Seedlings wer...

  18. A transcriptome-snp-derived linkage map of Apios americana (potato bean) provides insights about genome re-organization and synteny conservation in the phaseolid legumes

    Science.gov (United States)

    Apios (Apios americana; “apios”), a tuberous perennial legume in the Phaseoleae tribe, was widely used as a food by Native Americans. Work in the last 40 years has led to several improved breeding lines. Aspects of the pollination biology (complex floral structure and tripping mechanism) have made c...

  19. Physiological and foliar symptom response of Prunus serotina, Fraxinus americana and Acer rubrum canopy trees to ozone under differing site conditions

    Science.gov (United States)

    M. Schaub; J.M. Skelly; J.W. Zhang; J.A. Ferdinand; J.E. Savage; R.E. Stevenson; D.D. Davis; K.C. Steiner

    2005-01-01

    The crowns of five canopy dominant black cherry ( Prunus serotina Ehrh.), five white ash ( Fraxinus americana L.), and six red maple ( Acer rubrum L.) trees on naturally differing environmental conditions were accessed with scaffold towers within a mixed hardwood forest stand in central Pennsylvania....

  20. Immunotherapy, immunochemotherapy and chemotherapy for American cutaneous leishmaniasis treatment Imunoterapia, imunoquimioterapia e quimioterapia no tratamento da leishmaniose tegumentar americana

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    Wilson Mayrink

    2006-02-01

    Full Text Available The first choice of treatment for American cutaneous leishmaniasis is the pentavalent antimonial drug. Although it has been shown that this treatment is mostly effective and indicated, some disadvantages should be taken into account such as side effects, long term treatment inconveniences and counter-indication for patients suffering from cardiopathy, nephropathy; yet, aging, pregnancy and other conditions. With the advent of the vaccine anti-American cutaneous leishmaniasis as a prophylactic measure, studies on therapy using the vaccine associated or not with other drugs have been performed by many investigators and it is currently among the alternative treatments and prevention measures for American cutaneous leishmaniasis. In conclusion, the association between antimony and vaccine (immunochemotherapy showed the same cure rate when compared with the standard treatment (100% and it was also able to reduce the salt volume in 17.9% and treatment length from 87 to 62 days, decreasing side effects.O tratamento de primeira escolha para leishmaniose tegumentar americana é o antimonial pentavalente. Embora este tratamento seja na maioria das vezes efetivo e indicado, devem ser consideradas as desvantagens tais como efeitos colaterais, longa duração do tratamento e contra-indicação para cardiopatas, nefropatas, idosos, grávidas e outras condições. Com o advento da vacina antileishmaniose tegumentar americana para fins profiláticos e terapêuticos, associando-a ou não a outros fármacos, muitas pesquisas têm sido desenvolvidas, sendo a vacina a principal entre os atuais recursos no tratamento e prevenção da leishmaniose tegumentar americana. Em conclusão, a associação do antimônio com a vacina (imunoquimioterapia apresentou o mesmo índice de cura em relação ao tratamento padrão (100%, e ainda reduziu o volume do sal em 17,9% e o tempo de cura significativamente, de 87 para 62 dias; conseqüentemente, reduzindo os efeitos colaterais.

  1. EFICÁCIA DA FONOFORESE COM XIMENIA AMERICANA L. NA INFLAMAÇÃO DE TENDÃO DE RATOS

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    Seânia Santos Leal

    Full Text Available RESUMO Introdução: Diversos recursos terapêuticos, como laser e ultrassom isolado, combinados com fármacos e fonoforese têm sido utilizados em casos de inflamação e reparo de tendão, sendo o ultrassom pulsado bastante eficaz em tendinites crônicas. Objetivo: Analisar a eficácia do uso da fonoforese com o extrato etanólico das cascas do caule da Ximenia americana L. na resolução do processo inflamatório crônico em Rattus norvegicus. Métodos: A amostra consistiu em 120 animais para análise da resposta inflamatória, utilizando-se como variáveis edema, força biomecânica, número de fibroblastos e análise histológica. Resultados: Nos 7° e no 14° dia, verificou-se diferença significativa entre o grupo ultrassom com gel do caule de Ximenia americana L. e o grupo ultrassom com placebo (p < 0,05 quanto à redução de edema, aumento da força máxima de ruptura, redução extremamente significativa da deformação máxima (p < 0,001, além de aumento dos fibroblastos. Na análise histológica, houve melhora do processo inflamatório inicial e aceleração do reparo tendíneo, com redução de células inflamatórias e com deposição de colágeno organizado com matriz extracelular densa. Conclusão: O ultrassom pulsado combinado com o gel do caule de Ximenia americana L. é uma forma terapêutica eficaz para a resolução do processo inflamatório crônico.

  2. La Biología Molecular y sus aplicaciones en el estudio de la Tripanosomiasis Americana

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    Gustavo Adolfo Vallejo

    2000-08-01

    Full Text Available

    OTROS AUTORES:

    Dr. Gustavo Adolfo Vallejo
    José César Jaramillo
    Julio César Carranza
    Jorge Lorenzo Sánchez
    Leyder Helena Lozano
    Diego Gualtero
    Froylán Guayara
    Ludwin Andrés Cuervo
    Maria Teresa Mojica
    Carlos Alberto Jaramillo
    Académico Dr. Felipe Guh

    RESUMEN:

    Durante los últimos 35 años se ha experimentado un importante incremento de las publicaciones internacionales sobre la Tripanosomiosis Americana. Una reciente búsqueda en la base de datos MEDLINE por internet utilizando las palabras claves “Trypanosoma cruzi”, reveló 49 publicaciones en el quinquenio 1964-1968 con un incremento gradual hasta alcanzar la cifra de 1197 artículos internacionales en el quinquenio 1994-1998 (Figura 1. Sin embargo en cada uno de los países situados en las zonas endémicas de T. cruzi, existen numerosas publicaciones en revistas locales no indexadas cuya reseña queda excluida del MEDLINE.

    Sin embargo, el análisis de las publicaciones en el ámbito internacional permite identificar los paradigmas, los retos y los nuevos enfoques metodológicos que motivan y desafían a los diferentes grupos de investigación. Son numerosos los avances efectuados en estos último 35 años en área tan variadas como la biología celular, la inmunología, la inmunopatología, la bioquímica, la biología molecular y la quimioterapia de T. cruzi, así como también en estudio de los vectores de la enfermedad de Chagas.

    Los últimos 20 años se han caracterizado por un vigoroso auge de la investigación en bioquímica y biología molecular y el desarrollo de nuevas metodologías de genética molecular aplicadas exitosamente en los diferentes campos de estudio de la

  3. Female gonadal hormones and reproductive behaviors as key determinants of successful reproductive output of breeding whooping cranes (Grus americana).

    Science.gov (United States)

    Brown, Megan E; Converse, Sarah J; Chandler, Jane N; Shafer, Charles; Brown, Janine L; Keefer, Carol L; Songsasen, Nucharin

    2016-05-01

    Reproductive success of endangered whooping cranes (Grus americana) maintained ex situ is poor. As part of an effort to identify potential causes of poor reproductive success in a captive colony, we used non-invasive endocrine monitoring to assess gonadal and adrenal steroids of bird pairs with various reproductive outcomes and evaluated the relationships of hormones and behaviors to reproductive performance. Overall, reproductively successful (i.e., egg laying) females had significantly higher mean estrogen levels but lower mean progestogen concentrations than did unsuccessful females. Other hormones, including glucocorticoids and androgens, were not significantly different between successful and unsuccessful individuals. Observations of specific behaviors such as unison calling, marching, and the number of copulation attempts, along with overall time spent performing reproductive behaviors, were significantly higher in successful pairs. Our findings indicate that overall reproductive performance of whooping crane pairs is linked to female gonadal hormone excretion and reproductive behaviors, but not to altered adrenal hormone production. Published by Elsevier Inc.

  4. Occurrence of antibodies to Toxoplasma gondii in rheas (Rhea americana and ostriches (Struthio camelus from farms of different Brazilian regions

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    Amanda Brentano Almeida

    Full Text Available This study aimed to verify the occurrence of antibodies againstToxoplasma gondii in rheas (Rhea americana and ostriches (Struthio camelus commercially breeding in Brazil. Blood samples from 20 rheas and 46 ostriches (young and adults were serologically tested using a technique known as modified agglutination test (MAT at an initial titration of 1:16 for ostriches and 1:25 for rheas. Antibodies against T. gondii were found in 50% (10/20 of the rheas, with titers ranging from 1:25 to 1:6,400. The incidence of antibodies against T. gondii in ostriches was 17.4% (8/46 with titers ranging from 1:16 to 1:256. Birds showing titers higher than 1:200 forT. gondii were mainly the young ones. Therefore, rheas and ostriches may be parasitized by T. gondii, showing high levels of antibodies against this parasite.

  5. A new coccidian, Isospora rheae sp. nov. (Apicomplexa, Eimeriidae, from Rhea americana (Aves, Rheidae from South America

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    Samira S.M. Gallo

    2014-12-01

    Full Text Available A new species of coccidian (Protozoa: Apicomplexa: Eimeriidae obtained from rheas, Rhea americana, is reported in Brazil. Oocysts of Isospora rheae sp. nov. are spherical to subspheroidal, measuring 22.6 × 21.0 µm, and have a double and smooth wall that is approximately 1.7 µm thick. The micropyle, oocyst residuum and polar granule are absent. Sporocysts are slightly ovoid, measuring 13.9 × 9.6 µm. The Stieda body is flattened, the substieda body is pointed, irregular and wavy and the sporocyst residuum is composed of scattered granules of varying sizes. Sporozoites have an oblong refractile body and one nucleus. This is the first description of an isosporid coccidian infecting birds of the family Rheidae.

  6. Female gonadal hormones and reproductive behaviors as key determinants of successful reproductive output of breeding whooping cranes (Grus americana)

    Science.gov (United States)

    Brown, Megan E; Converse, Sarah J.; Chandler, Jane N.; Shafer, Charles; Brown, Janine L; Keefer, Carol L; Songsasen, Nucharin

    2016-01-01

    Reproductive success of endangered whooping cranes (Grus americana) maintained ex situ is poor. As part of an effort to identify potential causes of poor reproductive success in a captive colony, we used non-invasive endocrine monitoring to assess gonadal and adrenal steroids of bird pairs with various reproductive outcomes and evaluated the relationships of hormones and behaviors to reproductive performance. Overall, reproductively successful (i.e., egg laying) females had significantly higher mean estrogen levels but lower mean progestogen concentrations than did unsuccessful females. Other hormones, including glucocorticoids and androgens, were not significantly different between successful and unsuccessful individuals. Observations of specific behaviors such as unison calling, marching, and the number of copulation attempts, along with overall time spent performing reproductive behaviors, were significantly higher in successful pairs. Our findings indicate that overall reproductive performance of whooping crane pairs is linked to female gonadal hormone excretion and reproductive behaviors, but not to altered adrenal hormone production.

  7. Endoparasites of American marten (Martes americana: Review of the literature and parasite survey of reintroduced American marten in Michigan

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    Maria C. Spriggs

    2016-12-01

    Full Text Available The American marten (Martes americana was reintroduced to both the Upper (UP and northern Lower Peninsula (NLP of Michigan during the 20th century. This is the first report of endoparasites of American marten from the NLP. Faeces from live-trapped American marten were examined for the presence of parasitic ova, and blood samples were obtained for haematocrit evaluation. The most prevalent parasites were Capillaria and Alaria species. Helminth parasites reported in American marten for the first time include Eucoleus boehmi, hookworm, and Hymenolepis and Strongyloides species. This is the first report of shedding of Sarcocystis species sporocysts in an American marten and identification of 2 coccidian parasites, Cystoisospora and Eimeria species. The pathologic and zoonotic potential of each parasite species is discussed, and previous reports of endoparasites of the American marten in North America are reviewed.

  8. [The Unión Médica Hispano-Americana (1900) and its contribution to scientific internationalism].

    Science.gov (United States)

    Olagüe de Ros, Guillermo

    2006-01-01

    The Unión Médica Hispano-Americana was founded during the XIII International Congress of Medicine of Paris in 1900. Its main aim was to strengthen the cultural and scientific ties among Spanish speaking countries. The first assembly took place in Madrid in the Spring of 1903. The most interesting paper presented was undoubtedly that of Manuel Tolosa Latour. He suggested creating a Diccionario Tecnológico Médico Hispano-Americano. The society had no continuity and neither did the dictionary. The reasons for this failure must be seen in the absence of economic support by political authorities in Spain and America, the organizational weakness of the Unión and the poverty of its action program, which was imbued with a triumphalist rhetoric, empty of content and highly paternalistic in its relations with the American world.

  9. THE PERFORMANCE OF SOYBEAN (c.v. Americana ESTABLISHED BY ZERO TILLAGE TECHNIQUE IN IMPERATA FIELD CONTROLLED BY HERBICIDES

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    S. TJITROSEMITO

    1989-01-01

    Full Text Available A field experiment was conducted to investigate the performance of soybean (c.v. Americana when established with zero tillage technique on Imperata dominated area. Four different techniques of alang-alang control i.e. imazapyr (20 kg ai/ha, glyphosate (25 kg ai/ha, glufosinate (3.0 kg ai/ha and manual cultivation were arranged factorially with time of plantings i.e. 1,2 and 3 months after treatments. The alang-alang damages varied with herbicides and times, imazapyr (20 kg ai/ha showed slow appearance of damage at 3 months after application it was only 69%, while that of glufosinate was already down to 48% due to regrowth. No phytotoxicity was recorded, but the yield was low.

  10. Heavy metal and selenium levels in endangered wood storks Mycteria americana from nesting colonies in Florida and Costa Rica.

    Science.gov (United States)

    Burger, J; Rodgers, J A; Gochfeld, M

    1993-05-01

    Colonially-nesting birds often nest in coastal areas, along rivers, or near other bodies of water that also are potentially polluted from industrial, agricultural or urban development. The levels of heavy metals and selenium were examined in the feathers of young wood storks Mycteria americana nesting in Northeastern Florida and from adult and young storks nesting on the Tempisque River on the west coast of Costa Rica. There were no significant yearly differences among the chicks from Costa Rica. Concentration of mercury, cadmium, and lead were significantly higher in the chicks from Florida compared to those from Costa Rica. Adult wood storks at Costa Rica had significantly higher levels of lead, cadmium, selenium, and manganese than young from the same colony.

  11. Use of soil microorganisms as a biotechnological strategy to enhance avocado (persea americana)-plant phosphate uptake and growth / uso de microorganismos del suelo como estrategia biotecnológica para mejorar la absorción de fósforo y el crecimiento

    OpenAIRE

    Osorio Vega, Nelson Walter; Serna Gómez, Sandra Luz; Montoya Restrepo, Beatriz Elena

    2012-01-01

    In recent years the area cultivated with avocado has increased significantly in Colombia and this trend is expected to continue in the coming years. However, most of the soils in the highlands of the Colombian Andean, that have been used to grow avocado, are strongly acidic and nutrient deficient, particularly phosphorus (P). To alleviate these problems, high rates of lime and P fertilizers are frequently applied, in many cases the applied dose are excessive. This increases production cost...

  12. Tracking a genetic signal of extinction-recolonization events in a neotropical tree species: Vouacapoua americana Aublet in French Guiana.

    Science.gov (United States)

    Dutech, Cyril; Maggia, Laurent; Tardy, Christophe; Joly, Hélène I; Jarne, Philippe

    2003-12-01

    Drier periods from the late Pleistocene and early Holocene have been hypothesized to have caused the disappearance of various rainforest species over large geographical areas in South America and restricted the extant populations to mesic sites. Subsequent improvement in climatic conditions has been associated with recolonization. Changes in population size associated with these extinction-recolonization events should have affected genetic diversity within species. However, these historical hypotheses and their genetic consequences have rarely been tested in South America. Here, we examine the diversity of the chloroplast and nuclear genomes in a Neotropical rainforest tree species, Vouacapoua americana (Leguminosae, Caesalpinioideae) in French Guiana. The chloroplast diversity was analyzed using a polymerase chain reaction-restriction fragment length polymorphism method (six pairs of primers) in 29 populations distributed over most of French Guiana, and a subset of 17 populations was also analyzed at nine polymorphic microsatellite loci. To determine whether this species has experienced extinction-recolonization, we sampled populations in areas supposedly not or only slightly affected by climatic changes, where the populations would not have experienced frequent extinction, and in areas that appear to have been recently recolonized. In the putatively recolonized areas, we found patches of several thousands of hectares homogeneous for chloroplast variation that can be interpreted as the effect of recolonization processes from several geographical origins. In addition, we observed that, for both chloroplast and nuclear genomes, the populations in newly recolonized areas exhibited a significantly smaller allelic richness than others. Controlling for geographic distance, we also detected a significant correlation between chloroplast and nuclear population differentiation. This result indicates a cytonuclear disequilibrium that can be interpreted as a historical signal

  13. CARACTERIZAÇÃO QUÍMICA E FÍSICA DO JENIPAPO (Genipa americana L. ARMAZENADO

    Directory of Open Access Journals (Sweden)

    A. P. da SILVA

    1998-01-01

    Full Text Available As características do jenipapo (Genipa americana L. produzido na região de Botucatu-SP, bem como sua conservação pós-colheita foram estudados quando armazenado sob temperatura ambiente e sob refrigeração (10ºC. Os frutos foram avaliados a cada 7 dias quanto à perda de peso, diâmetro, vitamina C, sólidos solúveis totais, acidez total titulável, pH e firmeza. Ao final do período de armazenamento (28 dias de conservação, os resultados mostraram que a temperatura de 10ºC foi adequada para a conservação do jenipapo e, que as características químicas e físicas dos frutos produzidos na região de Botucatu, são comparativamente semelhantes aos produzidos em outras regiões produtoras, com exceção do teor de sólidos solúveis totais que estão abaixo dos relatados.To study the chemical and physical characteristics of jenipap (Genipa americana L. produced in the Botucatu-SP region, and its post-harvest conservation fruits, were studied when stored at ambient temperature and under refrigeration. The fruits were evaluated every 7 days for weight loss, diameter, vitamin C, total soluble solids, titratable acidity, pH and texture (flesh firmness. At the end of the 4th week of storage the results showed that the temperature of 10ºC was adequate for the conservation of jenipap, and that the chemical and physical characteristcs of the fruits produced in the Botucatu-SP region are similar to those produced in the other regions, except for total soluble solids, which was lower.

  14. Infecções experimentaes na Leishmaniose visceral americana Experimental infections in american visceral leishmaniasis

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    Aristides Marques da Cunha

    1938-01-01

    Full Text Available Passando em revista as experiencias de infecção que effectuamos e que acabamos de relatar, vemos que, as culturas isoladas de casos de Leishmaniose visceral americana quer do homem quer do cão, são capazes de infectar hamsters, rhesus e cães de maneira identica ao que acontece com as outras formas de Kala-Azar. Notamos ainda que a evolução da doença e as lesões observadas nos animaes assim infectados, se assemelham ao que tem sido observado nessa enfermidade aproximando-a de maneira singular do Kala-Azar mediterraneo. É sobretudo no cão que a semelhança se torna mais patente. A infecção da pelle e as lesões por ella provocadas que observamos, não differem em nada das que tem sido descriptas na infecção natural do cão e já assignaladas tambem na infecção experimental desse animal, embora de maneira menos completa do que fazemos agora aqui. As lesões oculares com formação de placas de keratite, a infeccção massiça do intestino nos casos fataes de infecção e até mesmo as lesões do figado descriptas por Adler como peculiares á infecção experimental, (infiltração em torno da veia central do lobulo constituem outros tantos caracteres que mostram a completa analogia entre as infecções do cão que obtivemos e as já observadas no Kala-Azar mediterraneo. Além disso, a presença de Leishmanias na pelle, as vezes em grande numero e a constancia dessa localisação parasitaria, vem mostrar que o cão apresenta as condições necessarias para desempenhar o papel de depositario de virus tal como acontece no Kala-Azar mediterraneo, tanto mais que é elle encontrado naturalmente infectado, nas regiões em que grassa a doença. Mas não é só a infecção do cão que mostra essa semelhança; nos outros animaes tambem se verifica o mesmo facto e até pequenos signaes, taes como a tumefacção das patas assignalada nos hamsters infectados com Leishmania infantum, foram tambem observados aqui. Por outro lado, a reacção de

  15. Technical review of the environmental study and report referent to the proposal of construction of a 1200 MW thermoelectric power plant (Carioba II) in the Americana district, SP, Brazil; Parecer tecnico parcial do estudo e relatorio de impacto ambiental (EIA/RIMA) referentes a proposta de implantacao de uma usina termeletrica de 1200 Mw (Carioba II) no municipio de Americana, SP

    Energy Technology Data Exchange (ETDEWEB)

    Figueiredo, Paulo Jorge Moraes; Martins, Gilberto [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil)]. E-mails: pfigueir@unimep.br; gmartins@unimep.br

    2001-06-01

    This document represents a partial analysis and a technical review about the environmental study and report of a 1200 MW thermoelectric power plant, proposed for the Americana district, SP, Brazil. Published on June, 4th 2001, this article points out many problems and mistakes of the referred studies, with the objective of providing support to the Federal attorney office and the general public. (author)

  16. Levantamento de plantas aquáticas no reservatório de Salto Grande, Americana-SP Aquatic plant survey in Salto Grande reservoir in Americana-SP, Brazil

    Directory of Open Access Journals (Sweden)

    D. Martins

    2011-03-01

    Full Text Available O objetivo deste estudo foi determinar a frequência relativa e o nível de infestação de cada espécie da flora aquática presente no reservatório de Salto Grande, Americana-SP. O levantamento e a identificação das plantas aquáticas foram realizados percorrendo-se as margens do reservatório em uma embarcação. Ao longo dele foram estabelecidos 20 pontos de avaliação, sendo todos eles fotografados e georreferenciados. Foram atribuídos valores de 0 a 100% tanto para as espécies presentes como para os espaços livres de macrófitas aquáticas que eventualmente pudessem ocorrer dentro dos pontos amostrados. Com os dados referentes ao número de indivíduos e pontos avaliados, foi determinada a frequência relativa de cada espécie. Foram identificadas 13 espécies em todo o reservatório, sendo 12 vasculares e uma de alga-verde (Chlorella spp.. Entre as espécies vasculares, nove eram plantas emersas flutuantes, as quais poderiam estar ou não ancoradas no leito do reservatório: Alternanthera philoxeroides, Brachiaria subquadripara, Cyperus difformis, Echinochloa polystachia var. spectabilis, Eichhornia crassipes, Panicum rivulare, Pistia stratiotes, Salvinia auriculata e Typha angustifolia. Outras três espécies foram encontradas somente em solo firme alagado: Aeschynomene sensitiva, Hedychium coronarium e Mimosa pigra.The objectives of this work were to determine the relative frequency and infestation level of each species present in the Salto Grande Reservoir in Americana-SP. The survey and identification of the aquatic plants were carried out in the Salto Grande reservoir on a boat. Twenty evaluation sites were established along the reservoir, photographed and geo-referenced. Values from 0 to 100% were assigned for both the species present and for the aquatic plants that could possibly occur within the sampled sites. Based on the number of individuals and points evaluated, the relative frequency of each species was determined

  17. Anti-motility and reductions in the concentrations of gut electrolytes ...

    African Journals Online (AJOL)

    The seeds of avocado (Persea americana Mill) are used in traditional medicine to treat, allay or prevent some spasm-related disorders, for instance, diarrhoea. The chloroform and methanol fractions of the chloroform-methanol extract of the seeds of P. americana were investigated for their qualitative and quantitative ...

  18. Partnerships Between Ambrosia Beetles and Fungi: Lineage-Specific Promiscuity Among Vectors of the Laurel Wilt Pathogen, Raffaelea lauricola.

    Science.gov (United States)

    Saucedo-Carabez, J R; Ploetz, Randy C; Konkol, J L; Carrillo, D; Gazis, R

    2018-04-20

    Nutritional mutualisms that ambrosia beetles have with fungi are poorly understood. Although these interactions were initially thought to be specific associations with a primary symbiont, there is increasing evidence that some of these fungi are associated with, and move among, multiple beetle partners. We examined culturable fungi recovered from mycangia of ambrosia beetles associated with trees of Persea humilis (silk bay, one site) and P. americana (avocado, six commercial orchards) that were affected by laurel wilt, an invasive disease caused by a symbiont, Raffaelea lauricola, of an Asian ambrosia beetle, Xyleborus glabratus. Fungi were isolated from 20 adult females of X. glabratus from silk bay and 70 each of Xyleborus affinis, Xyleborus bispinatus, Xyleborus volvulus, Xyleborinus saxesenii, and Xylosandrus crassiusculus from avocado. With partial sequences of ribosomal (LSU and SSU) and nuclear (β-tubulin) genes, one to several operational taxonomic units (OTUs) of fungi were identified in assayed individuals. Distinct populations of fungi were recovered from each of the examined beetle species. Raffaelea lauricola was present in all beetles except X. saxesenii and X. crassiusculus, and Raffaelea spp. predominated in Xyleborus spp. Raffaelea arxii, R. subalba, and R. subfusca were present in more than a single species of Xyleborus, and R. arxii was the most abundant symbiont in both X. affinis and X. volvulus. Raffaelea aguacate was detected for the first time in an ambrosia beetle (X. bispinatus). Yeasts (Ascomycota, Saccharomycotina) were found consistently in the mycangia of the examined beetles, and distinct, putatively co-adapted populations of these fungi were associated with each beetle species. Greater understandings are needed for how mycangia in ambrosia beetles interact with fungi, including yeasts which play currently underresearched roles in these insects.

  19. Avances de la fruticultura en México

    Directory of Open Access Journals (Sweden)

    Angel Villegas Monter

    2011-10-01

    Full Text Available Las estadísticas oficiales informaron que en 2008 se cultivaron 264 944 ha con valor de la producción de $ 14 741 millones de pesos, en 20 frutales de clima templado; mientras que, en 35 tropicales y subtropicales fue de 1 822748 ha con valor de $ 43 463 millones de pesos. De los 55 frutales, sólo naranjo (Citrus sinensis, mango (Mangifera indica, Aguacate (Persea americana, limón mexicano (C. aurantifolia, banano (Musa acuminata, lima persa (C. latifolia y manzano (Malus domestica se cultivan más de 50 000 ha, lo que explica porque es limitada la oferta de frutas en las grandes ciudades (Distrito Federal, Monterrey, Guadalajara, Puebla, Veracruz, entre otras. Considerando que el 79,27% de la superficie plantada con frutales tropicales y subtropicales es de temporal, las densidades de plantación son bajas, la propagación de plantas no se hace en viveros certificados (sólo cítricos, los rendimientos son bajos en comparación con otros países productores. Por otra parte, debido a la falta de técnicos capacitados, en los últimos 28 años la demanda de fruta se ha solucionado incrementando la superficie plantada, pero los rendimientos han disminuido. La presencia de nuevas enfermedades; Huanglongbing, Meleira, Sunblotch, plantean nuevos retos y también posibilidades si utilizamos los avances tecnológicos. La fruticultura es una alternativa viable pero deben utilizar altas densidades, árboles de porte bajo, plantas de origen genético conocido y calidad fitosanitaria probada que permitan tener mayores rendimientos y con ello competitividad.

  20. Introducao e expansao da Leishmaniose visceral americana em humanos no estado de Sao Paulo, 1999-2011

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    Marisa Furtado Mozini Cardim

    2013-08-01

    Full Text Available OBJETIVO : Analisar a expansão da ocorrência de leishmaniose visceral americana em humanos e identificar localidades prioritárias para o desenvolvimento de ações de vigilância e controle. MÉTODOS : A área de estudo constituiu-se dos 316 municípios do estado de São Paulo pertencentes às cinco regiões de saúde com ocorrência da leishmaniose visceral americana em humanos, utilizando os casos autóctones e óbitos, com ano de notificação e município de ocorrência. Calcularam-se taxas de incidência e de mortalidade e letalidade por município, por região e para a área de estudo. Utilizaram-se o estimador bayesiano empírico para obtenção de taxas de incidência e de mortalidade bayesianas locais para cada município e a krigagem para visualização da distribuição espacial das temperaturas e das precipitações pluviométricas. RESULTADOS : Foram detectados 73 municípios com transmissão da doença. As primeiras ocorrências deram-se em áreas com maiores temperaturas e menores pluviosidades, mas sua disseminação também ocorreu em áreas menos quentes e mais úmidas. A expansão da leishmaniose visceral americana em humanos apresentou um eixo principal de disseminação no sentido noroeste para sudeste, acompanhando a rodovia Marechal Rondon e o gasoduto Bolívia-Brasil, e um eixo secundário, na direção norte-sul, acompanhando a malha rodoviária. As taxas de incidência, segundo regiões de saúde, apresentaram um pico seguido de queda, com exceção da região de São José do Rio Preto. Observou-se maior concentração de municípios com altas taxas de incidência e mortalidade nas regiões de saúde de Araçatuba, Presidente Prudente e Marília. CONCLUSÕES : Possíveis fatores determinantes da expansão da doença incluíram a rodovia Marechal Rondon e a construção do gasoduto Bolívia-Brasil. Fatores climáticos pareceram não ter papel determinante nessa expansão. O uso de técnicas de análise espacial

  1. Specific B-cell Epitope of Per a 1: A Major Allergen of American Cockroach (Periplaneta americana) and Anatomical Localization.

    Science.gov (United States)

    Sookrung, Nitat; Khetsuphan, Thanyathon; Chaisri, Urai; Indrawattana, Nitaya; Reamtong, Onrapak; Chaicumpa, Wanpen; Tungtrongchitr, Anchalee

    2014-07-01

    Cockroach (CR) is a common source of indoor allergens, and Per a 1 is a major American CR (Periplaneta americana) allergen; however, several attributes of this protein remain unknown. This study identifies a novel specific B cell epitope and anatomical locations of Per a 1.0105. Recombinant Per a 1.0105 (rPer a 1.0105) was used as BALB/c mouse immunogen for the production of monoclonal antibodies (MAb). The MAb specific B cell epitope was identified by determining phage mimotopic peptides and pair-wise alignment of the peptides with the rPer a 1.0105 amino acid sequence. Locations of the Per a 1.0105 in P. americana were investigated by immunohistochemical staining. The rPer a 1.0105 (~13 kDa) had 100%, 98% and ≥90% identity to Per a 1.0105, Per a 1.0101, and Cr-PII, respectively. The B-cell epitope of the Per a 1.0105 specific-MAb was located at residues(99) QDLLLQLRDKGV(110) contained in all 5 Per a 1.01 isoforms and Per a 1.02. The epitope was analogous to the Bla g 1.02 epitope; however, this B-cell epitope was not an IgE inducer. Per a 1.0105 was found in the midgut and intestinal content of American CR but not in the other organs. The amount of the Per a 1 was ~544 ℃g per gram of feces. The novel Per a 1 B-cell epitope described in this study is a useful target for allergen quantification in samples; however, the specific MAb can be used as an allergen detection reagent. The MAb based-affinity resin can be made for allergen purification, and the so-purified protein can serve as a standard and diagnostic allergen as well as a therapeutic vaccine component. The finding that the Per a 1 is contained in the midgut and feces is useful to increase yield and purity when preparing this allergen.

  2. Aplicabilidade de geotecnologias para subsidiar a gestão dos recursos hídricos em Americana (SP / Applicability of geo-technologies to subsidize hydric resources management in the city of Americana (SP

    Directory of Open Access Journals (Sweden)

    Debora Cristina Cantador

    2017-05-01

    Full Text Available Ao analisar as águas urbanas tendo como recorte territorial o município, vários aspectos precisam ser levados em consideração. Assim pode-se entender como se realiza a gestão e, principalmente, como se encontra a qualidade, a preservação e a conservação dos cursos d’água. Esses aspectos devem estar relacionados, principalmente, com as legislações existentes que servem de subsídios para os gestores. Além disso, entende-se que a dinâmica espacial também auxilia na compreensão e visualização de como os recursos hídricos e os elementos associados a eles se comportam de forma mais ampla. Neste trabalho, buscou-se abordar sobre que modo as geotecnologias podem contribuir como um instrumento importante para a análise da atual situação da gestão dos recursos hídricos, usando como área de estudo o município de Americana (SP.

  3. Haemosporida prevalence and diversity are similar in endangered wild whooping cranes (Grus americana) and sympatric sandhill cranes (Grus canadensis).

    Science.gov (United States)

    Bertram, Miranda R; Hamer, Gabriel L; Hartup, Barry K; Snowden, Karen F; Medeiros, Matthew C; Hamer, Sarah A

    2017-04-01

    The population growth of endangered whooping cranes (Grus americana) is not consistent with species recovery goals, and the impact of parasite infection on whooping crane populations is largely unknown. Disease ecology and epidemiology research of endangered species is often hindered by limited ability to conduct invasive sampling on the target taxa. Accordingly, we hypothesized that sandhill cranes (Grus canadensis) would be a useful surrogate species to investigate the health impacts of Haemosporida infection in whooping cranes. Our goal was to compare the prevalence and diversity of Haemosporida infection between whooping cranes and sandhill cranes. We detected an overall infection prevalence of 83·6% (n = 61) in whooping cranes and 59·6% (n = 47) and 63·6 (n = 22) in two sympatric sandhill crane populations captured in Texas. Prevalence was significantly lower in allopatric sandhill cranes captured in New Mexico (12·1%, n = 33). Haemoproteus antigonis was the most abundant haemoparasite in cranes, present in 57·4% of whooping cranes and 39·2% of sandhill cranes; Plasmodium and Leucocytozoon were present at significantly lower levels. The high prevalence of Haemosporida in whooping cranes and sympatric sandhill cranes, with shared parasite lineages between the two species, supports sandhill cranes as a surrogate species for understanding health threats to endangered whooping cranes.

  4. Potential production and growth analysis of local variety and Americana variety of soybean (Glycine max (L) Merril)

    International Nuclear Information System (INIS)

    Sumakud, M.Y.M.A.

    2000-01-01

    Soybean needs high radiation intensity for photosynthesis process; therefore 100 percent of the sunlight are needed. Due to increasing the soybean production, the environment factor such as climate, soil and management are needed. One of the environment factor that influence the growth and dry matter production is radiation. This research done was to see the potential production of local variety and Americana variety by observing the total radiation absorption, temperature, rainfall and humidity. Therefore the objective of this research was to know the potential production in tropic area in soybean. If the production is mainly determined by the high growth rate or the length of phase linear or both of them also by the efficiency of radiation that received by the plant. The method of this research was carried out by using completed randomized design, with three replications. The result showed that the growth and the production of soybean are determined by growth rate (Cm) and the length of growth linear (tm). Dry matter of soybean is determined by growth rate instead of the length of growth linear, for efficiency of radiation are not significant. Pod formation is determined by the growth rate, content of pods is determined by the length of linear growth

  5. Biological Responses of the American Coot (Fulica americana), in wetlands with contrasting environmental conditions (Basin of México).

    Science.gov (United States)

    López-Islas, María Eugenia; Ibarra-Meza, Itzel; Ortiz-Ordóñez, Esperanza; Favari, Liliana; Elías Sedeño-Díaz, J; López-López, Eugenia

    2017-01-01

    Wetland ecosystems are subject to severe impacts (physical and chemical) and to the input of various xenobiotics that provoke toxicological consequences. Waterbirds are potential sentinel species of these environments. To analyze how habitat conditions affect the health of Fulica americana, early-warning biomarkers, histopathology, somatic indices, and water quality were examined in two wetlands of the Basin of Mexico: Xochimilco, an urban wetland highly eutrophic with a mixture of pollutants, and Tecocomulco (the reference site), a rural wetland with hunting migratory birds in winter, and with some agricultural contaminants. Coots were collected over 1 year, and the birds were aged, eviscerated, and weighed. Liver samples were analyzed biochemically and histologically. Biomarkers revealed that coots displayed higher lipid peroxidation and elevated activities of gamma-glutamyl transferase and alanine aminotransferase, suggesting hepatic damage during autumn and winter. In Tecocomulco, coots during winter has the highest thiobarbituric acid reactive substances (as a measure of oxidative stress), which may be associated with the presence of predators. In Tecocomulco, the higher gonadosomatic index was detected in spring and summer, while in Xochimilco it was elevated in summer, indicating a delayed egg laying and reproduction in coots from the latter study site. In both wetlands, leukocyte infiltration, alone or combined with vasocongestion, reflected alterations in the inflammatory processes in liver throughout the annual cycle and thus potentially altered hepatic function and organism survival. In both wetlands, coots were permanent residents and chronically exposed to different stressors, suggesting damage may be irreversible with potentially adversely reproductive consequences.

  6. Interplay between a Wnt-dependent organiser and the Notch segmentation clock regulates posterior development in Periplaneta americana

    Directory of Open Access Journals (Sweden)

    John E. Chesebro

    2012-12-01

    Sequential addition of segments in the posteriorly growing end of the embryo is a developmental mechanism common to many bilaterians. However, posterior growth and patterning in most animals also entails the establishment of a ‘posterior organiser’ that expresses the Caudal and Wnt proteins and has been proposed to be an ancestral feature of animal development. We have studied the functional relationships between the Wnt-driven organiser and the segmentation mechanisms in a basal insect, the cockroach Periplaneta americana. Here, posteriorly-expressed Wnt1 promotes caudal and Delta expression early in development to generate a growth zone from which segments will later bud off. caudal maintains the undifferentiated growth zone by dampening Delta expression, and hence Notch-mediated segmentation occurs just outside the caudal domain. In turn, Delta expression maintains Wnt1, maintaining this posterior gene network until all segments have formed. This feedback between caudal, Wnt and Notch-signalling in regulating growth and segmentation seems conserved in other arthropods, with some aspects found even in vertebrates. Thus our findings not only support an ancestral Wnt posterior organiser, but also impinge on the proposals for a common origin of segmentation in arthropods, annelids and vertebrates.

  7. Aspectos eco-epidemiológicos da leishmaniose tegumentar americana no Município de Campinas

    Directory of Open Access Journals (Sweden)

    Corte Antônio Ângelo

    1996-01-01

    Full Text Available Os autores descrevem um surto de leishmaniose tegumentar americana ocorrido no período de fevereiro de 1993 a setembro de 1994, nos Distritos de Sousas e Joaquim Egídio, Município de Campinas, SP. Ocorreram 25 casos, sete na área rural, nove na área peri-urbana e nove na urbana. Verifica-se uma mobilidade da ocorrência dos casos em direção rural-urbana. Em relação ao sexo, houve predomínio do masculino (60% sobre o feminino (40%, com 44% dos casos concentrados na faixa etária de 11 e trinta anos. Quanto à ocupação, 48% correspondem a estudantes, empregadas domésticas e donas-de-casa. Em 92% dos casos, a doença manifestou-se com lesão única, predominantemente localizadas nos membros inferiores e superiores. O surgimento desses casos na área está provavelmente associado à expansão urbana ocorrida nas décadas de 70-80 e acelerada mais recentemente.

  8. Physiological and foliar injury responses of Prunus serotina, Fraxinus americana, and Acer rubrum seedlings to varying soil moisture and ozone

    International Nuclear Information System (INIS)

    Schaub, M.; Skelly, J.M.; Steiner, K.C.; Davis, D.D.; Pennypacker, S.P.; Zhang, J.; Ferdinand, J.A.; Savage, J.E.; Stevenson, R.E.

    2003-01-01

    High soil water availability favors ozone uptake, increases foliar injury, and exacerbates the negative ozone effect on gas exchange of seedlings of deciduous tree species. - Sixteen black cherry (Prunus serotina, Ehrh.), 10 white ash (Fraxinus americana, L.) and 10 red maple (Acer rubrum, L.) 1-year old seedlings were planted per plot in 1997 on a former nursery bed within 12 open-top chambers and six open plots. Seedlings were exposed to three different ozone scenarios (ambient air: 100% O 3 ; non-filtered air: 98% ambient O 3 ; charcoal-filtered air: 50% ambient O 3 ) within each of two different water regimes (nine plots irrigated, nine plots non-irrigated) during three growing seasons. During the 1998 and 1999 growing season, leaf gas exchange, plant water relations, and foliar injury were measured. Climatic data, ambient- and chamber-ozone-concentrations were monitored. We found that seedlings grown under irrigated conditions had similar (in 1998) but significantly higher gas exchange rates (in 1999) than seedlings grown within non-irrigated plots among similar ozone exposures. Cherry and ash had similar ozone uptake but cherry developed more ozone-induced injury (<34% affected leaf area, LAA) than ash (<5% LAA), while maple rarely showed foliar injury, indicating the species differed in ozone sensitivity. Significantly more severe injury on seedlings grown under irrigated conditions than seedlings grown under non-irrigated conditions demonstrated that soil moisture altered seedling responses to ambient ozone exposures

  9. Ecological carryover effects associated with partial migration in white perch (Morone americana) within the Hudson River Estuary

    Science.gov (United States)

    Gallagher, Brian K.; Piccoli, Philip M.; Secor, David H.

    2018-01-01

    Partial migration in complex life cycles allows environmental conditions experienced during one life-stage to interact with genetic thresholds and produce divergent spatial behaviors in the next stage. We evaluated partial migration over the entire life cycle of white perch, (Morone americana) within the Hudson River Estuary, combining otolith microchemistry, population demographics and environmental data analysis. Ecological carryover effects were used as a framework to test how environmental variation during the larval period influenced migration behaviors and growth characteristics in subsequent life-stages. Two annual cohorts of juveniles were classified based on whether they persisted in natal habitats (freshwater resident contingent) or dispersed into non-natal habitats (brackish water migratory contingent) as juveniles. The migratory contingent tended to hatch earlier and experience cooler temperatures as larvae, while the availability of zooplankton prey during the larval period appeared to influence growth dynamics before and after metamorphosis. Juvenile migration behaviors were reversible but usually persisted into adulthood. As juveniles, the consequences of partial migration on growth appeared to be modified by river flow, as demonstrated by the influence of a large storm event on feeding conditions in one of the study years. Migratory adults grew faster and attained larger maximum sizes, but may also experience higher rates of mortality. The interplay uncovered between life-stage transitions, conditional migration behaviors and habitat productivity throughout the life cycle shapes white perch population dynamics and will likely play an important role in responses to long-term environmental change.

  10. O planejamento do "Recreio nas Férias" na cidade Paulista de Americana Planning for the program "leisure during the school break" ("Recreio nas Férias" in the brazilian city of Americana, state of São Paulo

    Directory of Open Access Journals (Sweden)

    Nayara Torre de Almeida

    2012-06-01

    Full Text Available O presente artigo constitui um relato de experiência do processo de planejamento e implementação do projeto "Recreio nas Férias" em um dos núcleos do Programa Segundo Tempo, localizado no bairro Vila Jones na cidade Paulista de Americana. O Projeto "Recreio nas Férias" é uma iniciativa do Ministério do Esporte, implantada em 2009 com o objetivo de, no período de férias escolares, oferecer às crianças e adolescentes participantes do programa, opções de esporte e lazer (e.g., atividades lúdicas, esportivas, artísticas, culturais, sociais e turísticas que "preencham o seu tempo livre de forma prazerosa e ao mesmo tempo construtiva" (BRASIL, 2010. Confrontando tais objetivos com os estudos mais atuais no campo do lazer é possível perceber que, no campo das políticas públicas, ainda permanece a visão histórica que tende a tratar o lazer como ocupação do tempo ocioso, principalmente quando se trata das propostas voltadas às classes populares. Isso fica evidente na preocupação explícita de "preencher o tempo livre", enfatizando seu caráter ocupacional. Nesse sentido, tecemos algumas aproximações com estudos de Silva (2003, 2008 e Marcellino (1995, 2001, 2008 compreendendo o lazer como uma das possibilidades de vivência do lúdico e construção da cidadania, ou seja, buscando responder a seguinte questão: pode o lazer assumir outro papel, senão este "ocupacional" historicamente observado nas políticas de esporte e lazer voltadas às classes populares?This study reports the planning and the implementation of the program "Recreio nas Férias" in one of the centers of the program "Second Half" ("Programa Segundo Tempo", located in Vila Jones in the Brazilian city of Americana, State of São Paulo. The program "Leisure During the School Break" ("Recreio nas Férias" is a project of the Ministry of Sports, lounged in 2009. The aim was, during the school break, to provide children and adolescents with sports and leisure

  11. Behavioural responses of captive-born greater rheas Rhea americana Linnaeus (Rheiformes, Rheidae submitted to antipredator training Respostas comportamentais de emas cativas Rhea americana Linnaeus (Rheiformes, Rheidae submetidas a treinamentoanti-predação

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    Cristiano S. de Azevedo

    2006-03-01

    Full Text Available Human activities have been diminishing greater rhea Rhea americana (Linnaeus, 1758 populations throughout their natural distribution. The reintroductions of captive-born greater rheas have been tried but without success; since the individuals reintroduced were killed by predators. Captive-born animals that have been isolated from predators for many generations can lose their predator recognition abilities. To enhance the survival rates of the reintroduced animals, researchers are now using antipredator training techniques. We studied the response of 15 zoo-borne greater rheas to antipredator training. The animals were divided into three groups: two test groups and one control group. We ran 15 antipredator tests and four control testes with each group. Antipredator tests consisted of pairing a taxidermized predator model or a real predator (domestic dog with a simulation of a capture procedure. Control tests consisted of presenting the predator model (jaguar to the birds, after training but not associating it with an aversive event and recording behavioural responses. All tests were video-recorded and analysed a posteriori. Results showed that the trained rheas responded appropriately to the predators, becoming more vigilant and that there was considerable individual differences in response to antipredator training. The results demonstrated that antipredator training is effective and therefore an invaluable tool for reintroduction projects involving greater rheas. Furthermore, the methods employed in this research project should be applicable to other species of flightless birds.As atividades humanas têm diminuído as populações de emas Rhea americana (Linnaeus, 1758 por toda sua área de distribuição. Reintroduções de emas nascidas em cativeiro têm sido tentadas, mas sem sucesso, uma vez que os indivíduos reintroduzidos são mortos por predadores. Animais nascidos em cativeiro que foram isolados de seus predadores por várias gera

  12. Modelos de educação geral na experiência universitária americana

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    José Camilo dos Santos Filho

    2010-06-01

    Full Text Available http://dx.doi.org/10.5007/1983-4535.2010v3n1p57   Em conseqüência da descentralização e da flexibilidade do sistema de educação superior e da grande autonomia das instituições universitárias nos Estados Unidos, não há obrigação legal de adoção de um programa de educação geral comum ou padrão para todo o país ou para todo um Estado da Federação. Por um lado, as instituições gozam de ampla liberdade e autonomia para conceberem seus programas de educação geral, o que tem levado a uma considerável variedade de abordagens institucionais da educação geral. Por outro lado, esse processo não impositivo tem levado ao longo do tempo a uma progressiva homogeneização básica de pressuposições e padrões semelhantes, mediante o processo de imitação dos programas das instituições de maior prestígio nacional. Robert Newton (2000 destaca esse caráter descentralizador e flexível da educação superior americana e a existência de quase tantos programas de educação geral quantos são os `colleges` e universidades do país. No entanto, acredita que, descartando-se os detalhes desses programas, podem emergir certas pressuposições e padrões esclarecedores. Desse modo, Newton propõe três abordagens que, no seu entender, podem fornecer um contexto para o planejamento, a compreensão e o aprimoramento de programas de educação geral. Os três modelos de educação geral dominantes atualmente nas universidades e ´colleges´ americanos são: o modelo centrado na disciplina acadêmica, o centrado nos Grandes Livros e o centrado na formação do cidadão efetivo. O propósito deste trabalho é analisar esses três modelos de educação geral adotados por essas instituições, destacando suas raízes históricas e as características básicas de cada um, à luz dos princípios subjacentes aos programas de educação geral. Cabe salientar que a discussão teórica e a prática efetiva de mais de cem anos de educação geral

  13. Production of iceberg lettuce using mulches Produção de alface tipo americana sob coberturas de solo

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    Marcelo Fontanetti Verdial

    2001-12-01

    Full Text Available Mulches bring several benefits to lettuce cultivation. This work evaluated the effect of mulches on the cultivation of iceberg lettuce, cv. Lucy Brown. The treatment plots were: 1- no mulch and no weed control (control; 2- no mulch and weed control every 15 days; 3- sugarcane bagasse mulch of 2 cm thickness; 4- black plastic mulch; 5- double-faced plastic mulch (silver/black. Data were collected in regard to plant cycle when in the field, amount of chlorophyll in the leaves, productivity (individual weight per head and amount of nutrients absorbed by the leaves. Double-faced plastic mulch provides the highest productivity and the highest values for the amount of chlorophyll, nitrogen, phosphorus, sulfur, boron and iron accumulation in the leaves.A utilização da cobertura do solo pode trazer inúmeros benefícios ao cultivo de alface. Este experimento teve como objetivo avaliar o efeito de tipos de coberturas de solo no cultivo de alface tipo americana cv. Lucy Brown. Os tratamentos foram: 1- canteiro sem cobertura e sem capina (testemunha, 2- canteiro sem cobertura, com capina quinzenal, 3- canteiro com cobertura de bagaço de cana de 2 cm de espessura, 4- canteiro com cobertura de plástico preto, 5- canteiro com cobertura de plástico dupla face (prateado/preto. Foram avaliados o ciclo das plantas no campo, a quantidade de clorofila nas folhas, a produção em peso individual de cabeça e a quantidade de nutrientes absorvidos nas folhas. A utilização de cobertura plástica do tipo dupla face proporcionou os maiores valores médios de produção. Foi também o tratamento em que foram obtidos os maiores valores para quantidade de clorofila, nitrogênio, fósforo, enxofre, boro e ferro acumulado nas folhas.

  14. Supplemental feeding for ecotourism reverses diel activity and alters movement patterns and spatial distribution of the southern stingray, Dasyatis americana.

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    Mark J Corcoran

    Full Text Available Southern stingrays, Dasyatis americana, have been provided supplemental food in ecotourism operations at Stingray City Sandbar (SCS, Grand Cayman since 1986, with this site becoming one of the world's most famous and heavily visited marine wildlife interaction venues. Given expansion of marine wildlife interactive tourism worldwide, there are questions about the effects of such activities on the focal species and their ecosystems. We used a combination of acoustic telemetry and tag-recapture efforts to test the hypothesis that human-sourced supplemental feeding has altered stingray activity patterns and habitat use at SCS relative to wild animals at control sites. Secondarily, we also qualitatively estimated the population size of stingrays supporting this major ecotourism venue. Tag-recapture data indicated that a population of at least 164 stingrays, over 80% female, utilized the small area at SCS for prolonged periods of time. Examination of comparative movements of mature female stingrays at SCS and control sites revealed strong differences between the two groups: The fed animals demonstrated a notable inversion of diel activity, being constantly active during the day with little movement at night compared to the nocturnally active wild stingrays; The fed stingrays utilized significantly (p<0.05 smaller 24 hour activity spaces compared to wild conspecifics, staying in close proximity to the ecotourism site; Fed stingrays showed a high degree of overlap in their core activity spaces compared to wild stingrays which were largely solitary in the spaces utilized (72% vs. 3% overlap respectively. Supplemental feeding has strikingly altered movement behavior and spatial distribution of the stingrays, and generated an atypically high density of animals at SCS which could have downstream fitness costs for individuals and potentially broader ecosystem effects. These findings should help environmental managers plan mitigating measures for existing

  15. Emoções e guerras: da Micronésia às bases militares americanas

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    Clara Saraiva

    2007-11-01

    Full Text Available Catherine Lutz tem um PhD em antropologia cultural da Universidade de Harvard, é professora do Departamento de Antropologia da Brown University, e investigadora do Watson Institute for International Studies da mesma universidade. Os seus temas de pesquisa debruçam-se sobre a antropologia das emoções, construção do género e da noção de raça, teoria crítica, mudanças na democracia e, mais recentemente, guerra, grupos militares e sociedade, nomeadamente através do estudo de bases militares americanas e do retorno de militares americanos dissidentes da guerra do Iraque. Lutz conduziu igualmente pesquisa para organizações activistas e todas estas temáticas se relacionam com a vontade de compreender as articulações socais e culturais das construções de poder e de desigualdade. Algumas das suas obras, influentes no campo da antropologia das emoções, da antropologia cognitiva e da história política são Unnatural Emotions: Everyday Sentiments on a Micronesian Atoll and their Challenge to Western Theory; Language and the Politics of Emotion(com Abu-Lughod; Reading National Geographic; Homefront: a Military City and the American 20th Century; Local Democracy under Siege: Activism, Public Interests, and Private Politics; The Bases of the Empire. The Struggle against US Military Posts, além de vários artigos como "Empire is in the details" e "Making war at home in the United States: militarization and the current crisis". Nesta entrevista Lutz fala sobre o que tem sido o seu trabalho e as suas preocupações académicas mas também sociais e interventivas.

  16. Jagua blue derived from Genipa americana L. fruit: A natural alternative to commonly used blue food colorants?

    Science.gov (United States)

    Brauch, J E; Zapata-Porras, S P; Buchweitz, M; Aschoff, J K; Carle, R

    2016-11-01

    Due to consumers' increasing health awareness, food industry aims at replacing synthetic dyes by natural counterparts. The substitution of blue synthetic dyes is particularly challenging since current natural alternatives such as phycocyanin (Spirulina) suffer from poor stability. Jagua blue (produced from Genipa americana L. fruit) might represent a potential novel blue pigment source. However, only little is known about its color properties, and application in food systems. Therefore, the blue color and the stability of Jagua blue were assessed for the first time and compared to commonly used colorants, namely, Spirulina, brilliant blue FCF (Blue no. 1), and indigo carmine (Blue no. 2). The reaction rate of Jagua blue was independent of its concentration, confirming thermal degradation to follow first-order kinetics. Between pH 3.6 and 5.0, the color hue of Jagua blue solutions was similar to that of Blue no. 2. However, Jagua blue revealed markedly higher storage stabilities (t 1/2 =86-105days) than Blue no. 2 (t 1 /2 ≤9days) and was less susceptible to acidic pH of 3.6 (t 1 /2 =86days) than Spirulina (t 1 /2 =70days). High negative b* values (blueness) of colored gelatin gels were only obtained for Jagua blue and Spirulina, and the former exhibited higher light stabilities (t 1 /2 =15days) than Spirulina gels (t 1 /2 =4days). Our findings indicate Jagua blue to be a most promising alternative to synthetic dyes, providing relevant information regarding potential food applications. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. Emprego da associação sulfametoxazol + trimetoprim no tratamento da paracoccidioidose (blastomicose Sul-Americana

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    P. N. Pedrosa

    1974-06-01

    Full Text Available Acentuando a necessidade da continuação das pesquisas terapêuticas em pacientes com paracoccidioidose (blastomicose sul-americana, os autores apresentam 23 pacientes submetidos a diferentes esquemas terapêuticos, sendo 14 virgens de tratamento e 9 com uso prévio de uma ou mais drogas. A associação sulfametoxazol + trimetoprim ( SMZ + TMP foi empregada em 5 pacientes virgens de tratamento e 9 sulfa-resistentes. Outros sete foram submetidos ao esquema clássico com sulfadoxina. Os pacientes que não responderam aos dois esquemas anteriores, com exceção de dois casos inicialmente graves, receberam anfotericina B. A avaliação clínica, radiológica, micológica e sorológica a longo prazo não demonstrou vantagens no emprego de SMZ + TMP em substituição aos sulfamídicos, nos pacientes virgens de tratamento. Entretanto, a associação SMZ + TMP parece ser uma opção válida nos casos sulfa-resistentes, onde teria primazia, considerando-se a toxicidade e necessidade de controle em regime hospitalar da anfotericina B. Ressaltam ainda a boa tolerância clínica e laboratorial da associação SMZ + TMP em cursos terapêuticos prolongados de até 2 anos, quando empregadas em baixas doses de manutenção.

  18. Anticonvulsant and Antioxidant Effects of Tilia americana var. mexicana and Flavonoids Constituents in the Pentylenetetrazole-Induced Seizures

    Science.gov (United States)

    Cárdenas-Rodríguez, Noemí; González-Trujano, María Eva; Aguirre-Hernández, Eva; Ruíz-García, Matilde; Sampieri, Aristides; Coballase-Urrutia, Elvia; Carmona-Aparicio, Liliana

    2014-01-01

    Tilia genus is commonly used around the world for its central nervous system properties; it is prepared as tea and used as tranquilizing, anticonvulsant, and analgesic. In this study, anticonvulsant activity of the Tilia americana var. mexicana inflorescences and leaves was investigated by evaluating organic and aqueous extracts (100, 300, and 600 mg/kg, i.p.) and some flavonoids in the pentylenetetrazole-induced seizures in mice. Moreover, antioxidant effect of these extracts and flavonoids was examined in an in vitro study by using spectrophotometric technique. Significant activity was observed in the methanol extract from inflorescences. An HPLC analysis of the methanol extract from inflorescences and leaves of Tilia allowed demonstrating the respective presence of some partial responsible flavonoid constituents: quercetin (20.09 ± 1.20 μg/mg and 3.39 ± 0.10 μg/mg), rutin (3.52 ± 0.21 μg/mg and 8.94 ± 0.45 μg/mg), and isoquercitrin (1.74 ± 0.01 μg/mg and 1.24 ± 0.13 μg/mg). In addition, significant but different antioxidant properties were obtained among the flavonoids and the extracts investigated. Our results provide evidence of the anticonvulsant activity of Tilia reinforcing its utility for central nervous system diseases whose mechanism of action might involve partial antioxidant effects due to the presence of flavonoids. PMID:25197430

  19. Supplemental feeding for ecotourism reverses diel activity and alters movement patterns and spatial distribution of the southern stingray, Dasyatis americana.

    Science.gov (United States)

    Corcoran, Mark J; Wetherbee, Bradley M; Shivji, Mahmood S; Potenski, Matthew D; Chapman, Demian D; Harvey, Guy M

    2013-01-01

    Southern stingrays, Dasyatis americana, have been provided supplemental food in ecotourism operations at Stingray City Sandbar (SCS), Grand Cayman since 1986, with this site becoming one of the world's most famous and heavily visited marine wildlife interaction venues. Given expansion of marine wildlife interactive tourism worldwide, there are questions about the effects of such activities on the focal species and their ecosystems. We used a combination of acoustic telemetry and tag-recapture efforts to test the hypothesis that human-sourced supplemental feeding has altered stingray activity patterns and habitat use at SCS relative to wild animals at control sites. Secondarily, we also qualitatively estimated the population size of stingrays supporting this major ecotourism venue. Tag-recapture data indicated that a population of at least 164 stingrays, over 80% female, utilized the small area at SCS for prolonged periods of time. Examination of comparative movements of mature female stingrays at SCS and control sites revealed strong differences between the two groups: The fed animals demonstrated a notable inversion of diel activity, being constantly active during the day with little movement at night compared to the nocturnally active wild stingrays; The fed stingrays utilized significantly (pecotourism site; Fed stingrays showed a high degree of overlap in their core activity spaces compared to wild stingrays which were largely solitary in the spaces utilized (72% vs. 3% overlap respectively). Supplemental feeding has strikingly altered movement behavior and spatial distribution of the stingrays, and generated an atypically high density of animals at SCS which could have downstream fitness costs for individuals and potentially broader ecosystem effects. These findings should help environmental managers plan mitigating measures for existing operations, and develop precautionary policies regarding proposed feeding sites.

  20. Variabilidade genética de Genipa americana L. pertencente ao baixo curso do rio São Francisco

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    Allívia Rouse Carregosa Rabbani

    2012-06-01

    Full Text Available A utilização de marcadores genéticos em estudos de caracterização de ecossistemas florestais permite avanços no entendimento genético das populações naturais, bem como auxilia na definição de estratégias de recuperação ou restauração florestal. Este trabalho foi realizado com o objetivo de avaliar a diversidade genética entre 18 indivíduos de Jenipapo (Genipa americana L. procedentes da região do Baixo São Francisco sergipano por meio de marcadores RAPD. Para a amplificação do material genético foram utilizados 12 oligonucleotídeos para análise da diversidade. Pelo índice de Jaccard, a similaridade genética média (Sgm entre os indivíduos foi de 60,4% sendo que, a maior obtida foi identificada entre os indivíduos G11 e G12 (83,6%±0,03 e a menor entre os indivíduos G4 e G18 (36,5%, ±0,02. Com base na Sgm (78,4%, sete pares indivíduos foram considerados geneticamente semelhantes. A partir destas análises, os indivíduos podem ser utilizados como matrizes fornecedoras de sementes em combinação com outros indivíduos em programas de restauração de áreas degradadas, porém devem compor áreas distantes.

  1. Los cariotipos de Cologania grandiflora y Erythrina americana (Leguminosae- Papilionoideae-Phaseoleae de la Reserva Ecológica del Pedregal de San Ángel, México Karyotypes of Cologania grandiflora and Erythrina americana (Leguminosae-Papilionoideae- Phaseoleae of Reserva Ecológica del Pedregal de San Ángel, Mexico

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    Fernando Tapia-Pastrana

    2011-09-01

    Full Text Available Se analizaron citogenéticamente células provenientes de meristemos radiculares de 2 leguminosas, Cologania grandiflora y Erythrina americana, que en la actualidad están incluidas en la flora de la Reserva Ecológica del Pedregal de San Ángel, México, D. F., mediante una técnica de extendido en superficie y secado al aire para determinar los números cromosómicos somáticos. Por vez primera se obtuvieron la morfología cromosómica y otras características cuantitativas de los cariotipos en C. grandiflora (2n= 44= 26m +18sm y en E. americana (2n= 42= 36m + 4sm +2st sat, primeras también en ambos géneros. Cologania y Erythrina se reconocen como poliploides estabilizados y el hallazgo de un único par de cromosomas con satélites (dominancia nucleolar en las especies estudiadas aquí, favorece la opinión de un origen alopoliploide para estos taxa.Meristematic root cells from Cologania grandiflora and Erythrina americana from Reserva Ecológica del Pedregal de San Ángel, Distrito Federal, Mexico, were analyzed cytogenenetically using a surface-spreading and air-drying method. The somatic chromosome numbers were determined. Chromosome morphology and others quantitative features of the karyotypes obtained for first time in C. grandiflora (2n= 44= 26m + 18sm and E. americana (2n= 42= 36m + 4sm + 2st sat and also the first in both genera. Cologania and Erythrina are recognized as stabilized polyploids and the finding of just one pair of chromosomes with satellites (nucleolar dominance in the species analyzed here supports the view of allopolyploid origin of these taxa.

  2. Characterization of Mn-resistant endophytic bacteria from Mn-hyperaccumulator Phytolacca americana and their impact on Mn accumulation of hybrid penisetum.

    Science.gov (United States)

    Zhang, Wen-Hui; Chen, Wei; He, Lin-Yan; Wang, Qi; Sheng, Xia-Fang

    2015-10-01

    Three hundred Mn-resistant endophytic bacteria were isolated from the Mn-hyperaccumulator, Phytolacca americana, grown at different levels of Mn (0, 1, and 10mM) stress. Under no Mn stress, 90%, 92%, and 11% of the bacteria produced indole acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase, respectively. Under Mn stress, 68-94%, 91-92%, and 21-81% of the bacteria produced IAA, siderophore, and ACC deaminase, respectively. Greater percentages of ACC deaminase-producing bacteria were found in the Mn-treated P. americana. Furthermore, the ratios of IAA- and siderophore-producing bacteria were significantly higher in the Mn treated plant leaves, while the ratio of ACC deaminase-producing bacteria was significantly higher in the Mn treated-roots. Based on 16S rRNA gene sequence analysis, Mn-resistant bacteria were affiliated with 10 genera. In experiments involving hybrid penisetum grown in soils treated with 0 and 1000mgkg(-1) of Mn, inoculation with strain 1Y31 was found to increase the root (ranging from 6.4% to 18.3%) and above-ground tissue (ranging from 19.3% to 70.2%) mass and total Mn uptake of above-ground tissues (64%) compared to the control. Furthermore, inoculation with strain 1Y31 was found to increase the ratio of IAA-producing bacteria in the rhizosphere and bulk soils of hybrid penisetum grown in Mn-added soils. The results showed the effect of Mn stress on the ratio of the plant growth-promoting factor-producing endophytic bacteria of P. americana and highlighted the potential of endophytic bacterium as an inoculum for enhanced phytoremediation of Mn-polluted soils by hybrid penisetum plants. Copyright © 2015 Elsevier Inc. All rights reserved.

  3. Prevalence and Antibiotic Resistance of Gram-Negative Pathogenic Bacteria Species Isolated from Periplaneta americana and Blattella germanica in Varanasi, India.

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    D Leshan Wannigama

    2014-06-01

    Full Text Available Cockroaches are among the medically important pests found within the human habitations that cause serious public health problems. They may harbor a number of pathogenic bacteria on the external surface with antibiotic resistance. Hence, they are regarded as major microbial vectors. This study investigates the prevalence and antibiotic resistance of Gram-negative pathogenic bacteria species isolated from Periplaneta americana and Blattella germanica in Varanasi, India.Totally, 203 adult cockroaches were collected form 44 households and 52 food-handling establishments by trapping. Bacteriological examination of external surfaces of Pe. americana and Bl. germanica were carried out using standard method and antibiotics susceptibility profiles of the isolates were determined using Kirby-Bauer disc diffusion methods.Among the places, we found that 54% had cockroache infestation in households and 77% in food- handling establishments. There was no significant different between the overall bacteria load of the external surface in Pe. americana (64.04% and Bl. germanica (35.96%. However the predominant bacteria on cockroaches were Klebsiella pneumonia, Escherichia coli, Enterobacter aerogenes, and Pseudomonas aeruginosa. However, Kl. pneumoniae and Ps. aeruginosa were the most prevalent, drug-resistant strains were isolated from the cockroaches with 100% resistance to sulfamethoxazole/trimethoprim and ampicillin. For individual strains of bacteria, Escherichia coli was found to have multi-resistance to four antibiotic tested, Citrobacter freundii four, Enterobacter aerogenes and Proteus mirabilis to three.Cockroaches are uniformly distributed in domestic environment, which can be a possible vector for transmission of drug-resistant bacteria and food-borne diseases.

  4. Imagen de América Latina en "La Ilustración Española y Americana", en el siglo XIX

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    Lic. Vivian Jara

    1998-01-01

    Full Text Available 'La Ilustración Española y Americana' es, probablemente, la más sobresaliente publicación semanal que se conserva de la época, una de las de mayor tirada y de mayor difusión, tanto en el Viejo como en el Nuevo Continente, y probablemente también una de las de mayor perdurabilidad en el tiempo. Se publicaba en Madrid y era distribuida a todos los países de habla hispana. Para realizar este trabajo la autora trabajó con los ejemplares de La Ilustración Española y Americana desde 1876 y hasta 1881, inclusive, y con los Almanaques de La Ilustración conservados en la Biblioteca de la Universidad de La Laguna. El trabajo consistió en el relevamiento de imágenes de y/o referidas a América Latina. Con tal fin y bajo la dirección y tutoría del profesor Adrián Alemán de Armas, se elaboró una ficha de investigación que condensó los aspectos esenciales de dichas imágenes. Asimismo, se realizaron simultáneamente observaciones acerca de algunos aspectos más sobresalientes, en los artículos referidos a América Latina. Estos son los primeros resultados de dichas observaciones. Por supuesto, aún queda mucho por releer, re-analizar y elaborar. En principio, hemos tratado de determinar, desde una perspectiva cuantitativa, el porcentual de espacio asignado a América Latina, en las páginas de La Ilustración Española y Americana.

  5. Ações afirmativas na Educação Superior: o que acadêmicos brasileiros podem aprender da experiência americana = Affirmative action in Higher Education: what Brazilian academics can learn from the American experience

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    Somers, Patricia

    2009-01-01

    Full Text Available Este artigo discute a história das ações afirmativas na educação dos Estados Unidos, da filosofia das ações afirmativas e do mérito do discurso para a educação, bem como reflete sobre a experiência americana. Refere-se, também, ao acesso à Educação Superior de jovens provenientes de classes minoritárias, brasileira e americana, examinado a importância da conexão da educação e da qualidade de vida. Chama atenção, ainda, para as relações que podem ser feitas pela educação brasileira, considerando a experiência americana

  6. Occurrence of anti-Toxoplasma gondii antibodies in greater rheas (Rhea americana) at the Reproduction Centre for Wild Animals in Mossoró, Rio Grande do Norte, Brazil

    OpenAIRE

    SOARES, H.S.; ALVES, N.D.; PEREIRA, R.H.M.A.; MATOS, S.M.; PENA, H.F.J.; GENNARI, S.M.; FEIJÓ, F.M.C.; AMÓRA, S.S.A.; PEIXOTO, G.C.X.

    2010-01-01

    Anti-Toxoplasma gondii antibodies were evaluated in greater rheas (Rhea americana) from the Reproduction Centre for Wild Animals located at the Universidade Federal Rural do Semiárido in the city of Mossoró, Rio Grande do Norte, by the modified agglutination test (MAT ≥25). It was verified that, from the 69 examined birds, 4.3% (three rheas) tested positive. The research about ratites toxoplasmoses are scarce, this way, the results showed the importance of the T. gondii diagnosis ...

  7. The taruca (Hippocamelus antisensis) and the red brocket deer (Mazama americana) as intermediate hosts of Taenia hydatigena in Peru, morphological and molecular evidence.

    Science.gov (United States)

    Gomez-Puerta, Luis A; Pacheco, Joel; Gonzales-Viera, Omar; Lopez-Urbina, Maria T; Gonzalez, Armando E

    2015-09-15

    In the present report metacestodes were collected from the mesentery of a taruca (Hippocamelus antisensis) and from the omentum of a red brocket deer (Mazama americana) in Peru. Various metacestodes parameters, including rostellar hook characteristics, were measured. Molecular analysis was performed to amplify the mitochondrial cytochrome c oxidase subunit 1 gene from metacestode isolates. Metacestodes were identified as T. hydatigena by morphology and molecular methods. This constitutes the first molecular detection of T. hydatigena metacestodes in the taruca and the red brocket deer and demonstrates that these animal species are natural intermediate hosts for this parasite. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. Políticas e poéticas feministas : imagens em movimento sob a ótica de mulheres latino-americanas

    OpenAIRE

    Maria Célia Orlato Selem

    2013-01-01

    Resumo: A presente pesquisa busca identificar poéticas feministas no cinema de mulheres latino-americanas neste século XXI. Nesse intuito, foram selecionados os seguintes filmes: La teta asustada, de Claudia Llosa (Peru - 2009); Que tan lejos, de Tania Hermida (Equador - 2006); Rompecabezas, Natalia Smirnoff (Argentina, 2009); Entre nós, de Paola Mendoza e Gloria La Morte (Colômbia, 2009); Sonhos Roubados, de Sandra Werneck (Brasil, 2009); En la puta vida, de Beatriz Flores Silva (Uruguai, 20...

  9. O processo inovativo das indústrias processadoras de suco de laranja brasileiras e norte-americanas: uma comparação utilizando a análise de patentes

    OpenAIRE

    Rossi, Fabiana Ribeiro; Universidade Federal de São Carlos – Departamento de Engenharia de Produção; Torkomian, Ana Lúcia Vitale; Universidade Federal de São Carlos – Departamento de Engenharia de Produção

    2015-01-01

    Desde a década de 80, o Brasil lidera a produção mundial de suco de laranja seguido, desde então, pelos Estados Unidos da América (EUA). Em torno de 90% da produção nacional de suco é exportada, enquanto a produção norte-americana é utilizada basicamente para consumo interno. O presente estudo teve como objetivo comparar a dinâmica de patenteamento das indústrias processadoras de suco de laranja brasileiras e norte-americanas. Para isso, foi feita uma busca por patentes utilizando a base de d...

  10. Allergic contact dermatitis caused by a new temporary blue-black tattoo dye - sensitization to genipin from jagua (Genipa americana L.) fruit extract.

    Science.gov (United States)

    Bircher, Andreas J; Sigg, Rita; Scherer Hofmeier, Kathrin; Schlegel, Urs; Hauri, Urs

    2017-12-01

    Temporary tattoos made with an extract of the jagua fruit (Genipa americana L.) are becoming increasingly popular. It is claimed that it is 'dermatologically tested' and does not contain p-phenylenediamine. Extracts of jagua and gardenia fruits have been used by indigenous people in South America, as well as in traditional Chinese medicine, for centuries. Genipin is currently used for its cross-linking effect in the manufacture of polysaccharides, and is being investigated for its anti-inflammatory and other properties. To report the presence of the allergenic substance genipin in a self-administered temporary tattoo dye made from the fruit juice of jagua (Genipa americana L.). A 39-year-old female who repeatedly applied 'completely natural and 100% safe' Earth Jagua® tattoo, obtained via the internet, to her left hand developed allergic contact dermatitis within 6 weeks. Analysis of the dye showed the presence of geniposide and genipin. Patch tests with the dye and with its main components, including genipin, gave strong positive reactions to the latter. There was no sensitization to other ingredients or p-amino compounds. We report an extensively evaluated case of allergic contact dermatitis caused by a temporary Earth Jagua® tattoo. The allergen identified is genipin, a substance that is increasingly used for tattoos and as a therapeutic agent in medicine. This could result in an increase in the number of allergic reactions in the future. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  11. Behavioral profiles of the captive juvenile whooping crane (Grus americana) as an indicator of reintroduction behavior and survival

    Science.gov (United States)

    Kreger, M.D.

    2003-01-01

    Predation by bobcats (Lynx rufus) has been the greatest cause of mortality of whooping cranes (Grus americana) in the reintroduced population in Florida. This study investigated whether the behavior of juvenile cranes during captive rearing and shortly after release can be used to predict their chances of survival once released in the wild. This study also examined differences in behavior based on rearing treatments and whether differences observed during rearing continued at the release site. Experimental rearing treatments were parent reared (PR), hand reared (RR), and hand reared with exercise (HRE). Two annual cycles of cranes were observed from hatch to 20 weeks of age in captivity (n=56 birds). Post-release bebavioral data were collected at the release site for a minimum of two weeks (n=34 birds), with mortality data collected up to one year post release (n=38 birds). Behavioral time budgets were compared using repeated measures ANOVA. Logistic regression was used to build a model to identify behaviors that were associated with first-year survival. During rearing, PR birds were the most vigilant. There were no behavioral differences between HR and HRE birds. Generally, rearing treatments had few long-term effects on the post-release behavior of the birds. The main behavioral differences during rearing and after release were the frequency of bouts and the percentage of time spent performing different behaviors. This may be attributed to foraging strategies and adaptation from captive conditions to the wild. Survival was not related to rearing treatment. Fifty-five percent of the birds survived the first year post-release based upon data pooled over two years. During rearing, the frequency of foraging bouts was positively correlated to survival. Survival was negatively correlated to the frequency of walking bouts during rearing, and release weight of the birds. These correlations accounted for 32 percent of the variability in survival. At the release site, 20

  12. Epizootic vacuolar myelinopathy of the central nervous system of bald eagles (Haliaeetus leucocephalus) and American coots (Fulica americana)

    Science.gov (United States)

    Thomas, N.J.; Meteyer, C.U.; Sileo, L.

    1998-01-01

    Unprecedented mortality occurred in bald eagles (Haliaeetus leucocephalus) at DeGray Lake, Arkansas, during the winters of 1994-1995 and 1996-1997. The first eagles were found dead during November, soon after arrival from fall migration, and deaths continued into January during both episodes. In total, 29 eagles died at or near DeGray Lake in the winter of 1994-1995 and 26 died in the winter of 1996-1997; no eagle mortality was noted during the same months of the intervening winter or in the earlier history of the lake. During the mortality events, sick eagles were observed overflying perches or colliding with rock walls. Signs of incoordination and limb paresis were also observed in American coots (Fulica americana) during the episodes of eagle mortality, but mortality in coots was minimal. No consistent abnormalities were seen on gross necropsy of either species. No microscopic findings in organs other than the central nervous system (CNS) could explain the cause of death. By light microscopy, all 26 eagles examined and 62/77 (81%) coots had striking, diffuse, spongy degeneration of the white matter of the CNS. Vacuolation occurred in all myelinated CNS tissue, including the cerebellar folia and medulla oblongata, but was most prominent in the optic tectum. In the spinal cord, vacuoles were concentrated near the gray matter, and occasional swollen axons were seen. Vacuoles were uniformly present in optic nerves but were not evident in the retina or peripheral or autonomic nerves. Cellular inflammatory response to the lesion was distinctly lacking. Vacuoles were 8-50 microns in diameter and occurred individually, in clusters, or in rows. In sections stained by luxol fast blue/periodic acid-Schiff stain, the vacuoles were delimited and transected by myelin strands. Transmission electron microscopy revealed intramyelinic vacuoles formed in the myelin sheaths by splitting of one or more myelin lamellae at the intraperiodic line. This lesion is characteristic of

  13. Longitudinal processes in Salto Grande reservoir (Americana, SP, Brazil and its influence in the formation of compartment system

    Directory of Open Access Journals (Sweden)

    L. H. ZANATA

    Full Text Available Studies on the longitudinal processes in reservoirs, involving physical, chemical and biological processes have been thoroughly appraised, suggesting the existence of a longitudinal organization controlled by the entrance and circulation of water which inserts modifications in the structuring of the system. To evaluate this effect, the Salto Grande reservoir (Americana, SP was analyzed in 11 sampling stations in its longitudinal axis, in the rainy and dry seasons of 1997 considering the physical chemical and biological variables. Analyzing the results in agreement with the declining concentration degree of the river--barrage direction, a more significant correlation was verified in the dry period for total phosphorus (r² = 0.86, dissolved total phosphate (r² = 0.83, nitrite (r² = 0.93, inorganic phosphate (r² = 0.89, ammonium (r² = 0.84 and suspended material (r² = 0.85. In the rainy period, only nitrite (r² = 0.90 and conductivity (r² = 0.89 presented correlation with the distance of the dam, which demonstrates the effects of precipitation and the operational mechanism of the dam, as well as the distinction among the physical (sedimentation, chemical (oxidation and biological (decomposition processes in spatial heterogeneity of the system. These factors were decisive in the organization of these communities, with higher occurrence of rotifers and copepods in relation to cladocerans, the first ones being more abundant in the entrance of the Atibaia river, decreasing towards the dam direction, while copepods presented an inverse pattern. A distribution pattern similar to Copepoda was also verified for the Cladocera, evidencing a tendency to increase the density of organisms in the stations distant to the entrance of the Atibaia river, not being registered, however, a distribution gradient in the longitudinal axis, as observed for rotifers and copepods. In relation to the trophic degree a longitudinal gradient was also verified from

  14. Extração da mucilagem em sementes de Genipa americana L. visando o potencial fisiológico

    Directory of Open Access Journals (Sweden)

    Amanda Kelly Dias Bezerra

    Full Text Available RESUMOFrutífera pertencente à família Rubiaceae, o jenipapo (Genipa americana L. apresenta ampla distribuição em regiões tropicais úmidas e subtropicais da América Latina. Suas sementes apresentam densa mucilagem aderida, a qual pode prejudicar a germinação favorecendo ao desenvolvimento de microrganismos, requerendo desta forma, operações para a sua eliminação. Diante do exposto, objetivou-se avaliar o potencial fisiológico em lotes de sementes de jenipapo sob diferentes métodos de extração da mucilagem. Frutos provenientes de quatro matrizes de jenipapeiro do município de Areia, PB, foram submetidos aos métodos de extração da mucilagem das sementes: manual com peneira, água corrente e areia; fermentação; centrifugação e H2SO4. Avaliou-se o potencial fisiológico das sementes quanto ao teor de água, germinação, primeira contagem e índice de velocidade de germinação, comprimento e massa seca, emergência de plântulas e teste de sanidade. O delineamento experimental foi inteiramente casualizado e as médias comparadas pelo teste de Tukey (p < 0,05. O teor de água das sementes foi semelhante entre os lotes. Os diferentes métodos de extração da mucilagem de sementes de jenipapo avaliados não influenciaram a germinação, mantendo a viabilidade, entretanto, quando avaliadas quanto ao vigor, as sementes extraídas na peneira mostram-se superior quando comparadas aos outros métodos. Assim, o método da remoção da mucilagem das sementes de jenipapo em peneira com areia proporcionou sementes com alto potencial fisiológico, não havendo influência na qualidade sanitária destas sementes.

  15. First Report of ‘Candidatus Phytoplasma trifolii’-Related Strain of 16SrVI-A Phytoplasma Subgroup, Associated with Elm Yellows Disease in American Elm ( Ulmus americana L.) in Ohio, U.S.A

    Science.gov (United States)

    C.E. Flower; N. Hayes-Plazolles; J.M. Slavicek; C. Rosa

    2018-01-01

    During the investigation of the sudden and early onset of yellowing and mortality of American elm (Ulmus americana L.) trees at the USDA Forest Service Northern Research Station in Delaware, OH, a phytoplasma of the clover proliferation group (16SrVI) was detected as the putative causal agent of the disease outbreak.

  16. Callicarpa americana L.

    Science.gov (United States)

    K.F Connor

    2004-01-01

    American beautyberry is a shrub that can reach 2.4 to 2.7 m in height but is typically shorter, averaging 0.9 to 1.8 m. The irregular, spreading bush has an open growth habit and grows as a single plant or in colonies. Bark is brown and raised lenticels are found on older stems. The opposite, short-petioled, simple, deciduous leaves are ovate to elliptic in shape. They...

  17. Teaching German-Americana

    Science.gov (United States)

    Tolzmann, Don Heinrich

    1976-01-01

    A university course entitled "The German-Americans" attempted to study and evaluate German culture in the U. S. Lecture topics and term paper theses are listed and a selected annotated bibliography of German-American culture is included. (CHK)

  18. Growth and cesium uptake responses of Phytolacca americana Linn. and Amaranthus cruentus L. grown on cesium contaminated soil to elevated CO2 or inoculation with a plant growth promoting rhizobacterium Burkholderia sp. D54, or in combination

    International Nuclear Information System (INIS)

    Tang, Shirong; Liao, Shangqiang; Guo, Junkang; Song, Zhengguo; Wang, Ruigang; Zhou, Xiaomin

    2011-01-01

    Highlights: ► Elevated CO 2 and microbial inoculation, alone or in combination, significantly promoted growth of P. americana, and A. cruentus. ► Total tissue Cs in plants was significantly increased. ► A. cruentus had higher tissue Cs concentration, Cs transfer factors and concentration ratios than P. americana. ► The two plants had slightly different contents of antioxidant enzymes. ► Combined effects of elevated CO 2 and microbial inoculation can be explored for CO 2 - and microbe-assisted phytoextraction technology. - Abstract: Growth and cesium uptake responses of plants to elevated CO 2 and microbial inoculation, alone or in combination, can be explored for clean-up of contaminated soils, and this induced phytoextraction may be better than the natural process. The present study used open-top chambers to investigate combined effects of Burkholderia sp. D54 inoculation and elevated CO 2 (860 μL L −1 ) on growth and Cs uptake by Phytolacca americana and Amaranthus cruentus grown on soil spiked with various levels of Cs (0–1000 mg kg −1 ). Elevated CO 2 and bacterial inoculation, alone or in combination, significantly increased biomass production with increased magnitude, ranging from 22% to 139% for P. americana, and 14% to 254% for A. cruentus. Total tissue Cs in both plants was significantly greater for bacterial inoculation treatment singly, and combined treatments of bacterial inoculation and elevated CO 2 than for the control treatment in most cases. Regardless of CO 2 concentrations and bacterial inoculation, A. cruentus had higher tissue Cs concentration, Cs transfer factors and concentration ratios than P. americana, but they had slightly different contents of antioxidant enzymes. It is concluded that combined effects of elevated CO 2 and microbial inoculation with regard to plant ability to grow and remove radionuclides from soil can be explored for CO 2 - and microbe-assisted phytoextraction technology.

  19. Sustitución de lardo por grasa vegetal en salchichas: incorporación de pasta de aguacate. Efecto de la inhibición del oscurecimiento enzimático sobre el color Substituição do toucinho por gordura vegetal em salsichas: adição da pasta de abacate. Efeito da inibição do escurecimiento enzimatico na cor

    Directory of Open Access Journals (Sweden)

    Ubaldo Rueda-Lugo

    2006-06-01

    Full Text Available La sustitución de grasa animal es importante para mejorar la calidad nutricional de los alimentos de origen animal. Tradicionalmente, los embutidos contienen cantidades relativamente altas de grasas insaturadas, por lo que se ha buscado la sustitución parcial o total de estas con grasas o aceites de origen vegetal. El aprovechamiento del aguacate como fuente de grasa vegetal es una alternativa para este tipo de productos. La oxidación de este fruto es uno de los principales problemas durante la industrialización. En este trabajo se han añadido dos inhibidores del oscurecimiento enzimático, ácido ascórbico y eritorbato de sodio, para determinar el efecto de éstos sobre el color de salchichas de cerdo. Se han encontrado diferencias en la luminosidad de las muestras con respecto al tratamiento control, además de que este mismo parámetro se ha reducido con el tiempo de almacenamiento. No ha habido efecto significativo de los antioxidantes sobre las otras componentes del color. El uso de eritorbato de sodio en la elaboración de pasta de aguacate ha reducido el oscurecimiento de la pasta durante su congelación, sin afectar mayormente otros parámetros de color del producto terminado. La aceptación del producto por un grupo de consumidores ha sido aceptable.A substituição da gordura animal ou toucinho é importante para melhorar a qualidade nutricional dos alimentos de origem animal. Tradicionalmente, os embutidos contêm quantidades relativamente elevadas de gorduras insaturadas, e a substituição parcial ou total com gordura vegetal ou óleo é desejável. O abacate é fonte de gordura vegetal como uma alternativa para este tipo de produto. A oxidação desta fruta é um dos problemas principais durante a industrialização. Neste trabalho foram adicionados dois inibidores de escurecimento enzimatico, ácido ascórbico e eritorbato do sódio, para determinar o efeito destes na cor das salsichas. As diferenças na luminosidade das

  20. Ultrastructure and electrolyte transport of the epithelium of coprodeum, colon and the proctodeal diverticulum of Rhea americana

    DEFF Research Database (Denmark)

    Elbrønd, Vibeke Sødring; Laverty, Gary; Dantzer, Vibeke

    2009-01-01

    chloride secretion response, and ouabain, the Na+/K+-ATPase inhibitor, abolished most of the ISC. The transepithelial resistance (TER) of the diverticulum was much higher than the other tissues. Upon dissection, urate from ureteral urine was observed in the lower third of the colon and to a lesser extent......The structure and function of the lower intestinal tract of Rhea americana were characterized to evaluate the evolutionary relationship to other struthioniform and avian species. In 5 rheaqs the gross anatomy and the light and transmission electron microscopy were studied in parallel to in vitro...... large amounts of mucus. The proctodeal diverticulum was rich in lymphoid tissue arranged into lobuli bursales, and it was concluded that this structure is a modified bursa of Fabricius. The sparse interlobular epithelium of the diverticulum resembled that of colon and coprodeum. Baseline short circuit...

  1. Símbolos del Tiempo, la Identidad y la Alteridad en la Visión Americana de José Martí

    Directory of Open Access Journals (Sweden)

    Ricardo Melgar Bao

    2001-01-01

    Full Text Available Desde nuestro mirador antropológico, consideramos que el proceso de simbolización cobró par ticular visibilidad y desarrollo en la perspectiva americana de José Martí du rante los últimos quince años de su vida. Nos referimos particularmente al tiempo, la identidad y la alteridad presentes en su ensayo Nuestra América y algunos otros escritos. Sostenemos que el análisis de este cam po simbólico en el pensamiento de un autor, nos puede decir tanto o más que los enfoques tradicionales propios a la historia de las ideas en América Latina.

  2. Distinct localization of FMRFamide- and bovine pancreatic polypeptide-like material in the brain, retrocerebral complex and suboesophageal ganglion of the cockroach Periplaneta americana L

    DEFF Research Database (Denmark)

    Verhaert, P; Grimmelikhuijzen, C J; De Loof, A

    1985-01-01

    One bovine pancreatic polypeptide (BPP) antiserum and two FMRFamide antisera were applied in the peroxidase-antiperoxidase (PAP) immunohistochemical technique on a complete series of sections of brains, suboesophageal ganglia (SOG), corpora cardiaca (CC) and corpora allata of Periplaneta americana...... L. Double immunohistochemical staining demonstrated that the same perikarya and processes were stained by both the BPP and FMRFamide antisera. This was caused by cross-reaction of the BPP and FMRFamide antisera with common antigenic determinants as was shown by a number of solid-phase absorptions....... Application of a third FMRFamide antiserum, which was especially selected for its inability to react with bovine and avian pancreatic polypeptide, showed that more than half of the structures that were stained with the 'unspecific' BPP and FMRFamide antisera, contained material which was genuinely FMRFamide...

  3. Leishmaniose tegumentar americana na Ilha Grande. Rio de Janeiro: V. Observações sobre a biologia dos transmissores em condiçoes naturais

    Directory of Open Access Journals (Sweden)

    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Durante um surto de Leishmaniose Tegumentar Americana (LTA na Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, foram coletados 4.192 exemplares de flebotomíneos em ambiente domiciliar e silvestre, com isca animal e outros abrigos naturais, durante o período de janeiro de 1976 a abril de 1977. Entre o total de 11 espécies coletadas encontraram-se 2.493 exemplares de Lutzomyia intermedia, 1.594 de Lutzomyia migonei e 105 exemplares de outras espécies. A L. intermedia apresentou densidade mais elevada no domicílio humano, enquanto a L. migonei apresentou coletas mais rendosas com iscas animais e galinheiros. O sinantropismo e a elevada densidade no ambiente domiciliar incriminam as espécies L. intermedia e L. migonei como as prováveis vetoras da LTA na Ilha Grande.

  4. Apropiación y alienación: consideraciones hermenéutico-existenciales a propósito de la Pastoral americana de Philip Roth

    Directory of Open Access Journals (Sweden)

    Germán Darío Vélez López

    2013-01-01

    Full Text Available Partiendo de una formulación básica de algunos elementos relevantes de la hermenéutica fenomenológica de Heidegger, el presente artículo propone una lectura de la obra del escritor norteamericano Philip Roth titulada Pastoral americana, que lo hizo acreedor al premio Pulitzer en 1989. El propósito cen- tral de esa lectura consiste en mostrar de qué manera fenómenos existenciales elementales como estar-lejos (Wegsein, estar-despierto (Wachsein, estar-ahí (Dasein permiten una articulación propia del sentido de la obra de Roth en la que ésta manifiesta su particular compromiso existencial.

  5. La exposición histórico-americana de Madrid de 1892 y la ¿ausencia? de México

    Directory of Open Access Journals (Sweden)

    Ramírez Losada, Dení

    2009-08-01

    Full Text Available The Fourth Centennial of the Discovery of America was celebrated in 1892. Together with the rest of the festivities, the Historical American Exposition of Madrid is considered a landmark not only for its political, cultural and scientific relevance, which, to this day, have not been studied in their entirety, but as well for the implications that resulted for Mexico. This article is meant to show, on the one side, the singularity of such festivities, and, on the other, the fact that Mexico’s participation constituted an important historical moment for Mexican scientific anthropology. This research was essentially carried out based on the numerous documentation found at the Archivo General de la Administración, Alcalá de Henares.

    En 1892 se celebró en España el IV Centenario del Descubrimiento de América. En el marco de este festejo destacó, sin duda, la Exposición Histórico Americana de Madrid no sólo por su enorme relevancia política, cultural y científica que, hasta ahora, se ha estudiado poco, sino por todo aquello que significó para México en particular. El artículo centra su interés en destacar, por un lado, la singularidad de los festejos colombinos y, por el otro, mostrar que la participación de México en la Exposición Histórico-Americana constituyó todo un hito científico en la antropología mexicana. La investigación se llevó a cabo, fundamentalmente, sobre la copiosa documentación encontrada en el Archivo General de la Administración, Alcalá de Henares.

  6. Complete Genome Sequence of a Double-Stranded RNA Virus from Avocado

    OpenAIRE

    Villanueva, Francisco; Sabanadzovic, Sead; Valverde, Rodrigo A.; Navas-Castillo, Jesús

    2012-01-01

    A number of avocado (Persea americana) cultivars are known to contain high-molecular-weight double-stranded RNA (dsRNA) molecules for which a viral nature has been suggested, although sequence data are not available. Here we report the cloning and complete sequencing of a 13.5-kbp dsRNA virus isolated from avocado and show that it corresponds to the genome of a new species of the genus Endornavirus (family Endornaviridae), tentatively named Persea americana endornavirus (PaEV).

  7. Complete Genome Sequence of a Double-Stranded RNA Virus from Avocado

    Science.gov (United States)

    Villanueva, Francisco; Sabanadzovic, Sead; Valverde, Rodrigo A.

    2012-01-01

    A number of avocado (Persea americana) cultivars are known to contain high-molecular-weight double-stranded RNA (dsRNA) molecules for which a viral nature has been suggested, although sequence data are not available. Here we report the cloning and complete sequencing of a 13.5-kbp dsRNA virus isolated from avocado and show that it corresponds to the genome of a new species of the genus Endornavirus (family Endornaviridae), tentatively named Persea americana endornavirus (PaEV). PMID:22205720

  8. Wirkungen biogener Amine auf die Erregungs-Sekretions-Kopplung in der Speicheldrüse von Periplaneta americana (L.)

    Science.gov (United States)

    Rietdorf, Katja

    2003-07-01

    In der vorliegenden Arbeit habe ich wichtige Teilmechanismen der Erregungs-Sekretionskopplung in der Speicheldrüse der Schabe Periplaneta americana (L.) untersucht. Die Speicheldrüse ist von dopaminergen und serotonergen Fasern innerviert (Baumann et al., 2002). Beide Transmitter stimulieren eine unterschiedliche Reaktion der Drüse: Dopamin (DA) stimuliert die P-Zellen der Acini und die Ausführgangzellen, während Serotonin (5-HT) die P- und C-Zellen der Acini stimuliert, nicht jedoch die Ausführgangzellen. Der Endspeichel ist nach einer DA-Stimulierung proteinfrei. Dagegen enthält er nach einer 5-HT-Stimulierung Proteine, die von den C-Zellen sezerniert werden (Just & Walz, 1996). Im ersten Teil meiner Arbeit habe ich mittels Kapillarelektrophoretischer Analyse (CE-Analyse) die Elektrolytkonzentrationen im Endspeichel untersucht sowie die Raten der Flüssigkeitssekretion gemessen. Damit wollte ich klären, welche Transporter an der Sekretion des Primärspeichels und an dessen Modifikation beteiligt sind. Ausserdem wollte ich die Rolle der transportaktiven Epithelzellen der Ausführgänge für die Modifikation des Primärspeichels untersuchen. Dafür habe ich einen Vergleich der Elektrolytkonzentrationen im DA- und 5-HT-stimulierten Endspeichel durchgeführt. Der Elektrolytgehalt des DA- und 5-HT-stimulierten Endspeichels unterscheidet sich nicht signifikant voneinander. Er ist nach beiden Stimulierungen hypoosmotisch zum verwendeten Ringer. Die Ausführgangzellen werden durch DA stimuliert und modifizieren den Primärspeichel durch eine netto-Ionenreabsorption. Meine Versuche zeigen jedoch, dass auch die während einer 5-HT-Stimulierung der Drüse unstimulierten Ausführgangzellen den Primärspeichel modifizieren. In einer nachfolgenden Versuchsreihe habe ich den Einfluss von Ouabain, einem Hemmstoff der Na+-K+-ATPase, und Bumetanid, einem Hemmstoff des NKCC, auf die Raten der Flüssigkeitssekretion sowie den Elektrolytgehalt des Endspeichels untersucht. Ich

  9. Um foco potencial de Tripanosomíase Americana na cidade do Rio de Janeiro (Distrito Federal

    Directory of Open Access Journals (Sweden)

    F. Nery Guimarães

    1943-12-01

    Full Text Available São relatados estudos epidemiológicos feitos em um foco potencial de Tripanosomiase americana no bairro Santa Teresa na Cidade do Rio de Janeiro. Enquanto a pesquisa intensiva de Triatomideos em 40 "cafuas" existentes em áreas silvestres do local foi negativa, 11 exemplares (seis fêmeas e cinco machos de Panstrongylus mzgistus (Burmeister, 1835 foram capturados em um grande edifício do local, estando nove infectados pelo Trypanosoma (S. cruzi. Positivamente, tais insetos aí vieram ter atraídos pela grande iluminação dêsse edifício, seus focos de criação encontrando-se na mata. Não foi possível demonstrar de modo indiscutível tais criadouros em cuidadosas pesquisas feitas durante 10 meses. Todavia, em ninho de gambá (Didelphis marsupialis foi achado uma casca de ovo de Triatomídeo, o qual pode ser atribuído ao P. megistus. Também em ninhos dos mesmos marsupiais e também de ratos silvestres foi descoberto um novo Triatomídeo (Parabelminus carioca Lent, 1943 novo transmissor do Trypanosoma (S. cruzi, e que foi motivo de uma publicação anterior. De 42 exemplares de gambás (D. marsupialis submetidos ao exame direto do sangue e ao xenodiagnóstico, 15 (ou seja 35.7 % mostraram-se naturalmente infectados pelo T. (S. cruzi. Dêstes quinze, quatro foram negativos ao exame direto de sangue, mas positivos ao xenodiagnóstico. Outro marsupial (Metachirus nudicaudatus Geoffroy, antes ainda não referido como depositário silvestre do Trypanosoma (S. cruzi, foi verificado com infecção natural pelo xenodiagnóstico. A amostra "gambá” de T. (S. cruzi, mostrou-se patogênica para cao rhesus, gato e cobaio. Foi possível cultivá-la em meios de Nöller e NNN. A amostra "cuica" foi capaz de infectar cão e cobaio e também foi cultivada. A amostra "panstrongylus" também infectou cão e cobaio. Camondongos (Mus musculus, var albina inoculados com qualquer uma das amostras, não apresentaram infecção sanguínea apreciável ao exame

  10. Aspectos epidemiológicos da Leishmaniose Tegumentar Americana no município de Juína, Mato Grosso, Brasil = Epidemiological aspects of American Cutaneous Leishmaniasis in the city of Juína, Mato Grosso state, Brazil

    Directory of Open Access Journals (Sweden)

    França, Eduardo Luzia

    2009-01-01

    Conclusões: a análise dos dados revelou que a Leishmaniose Tegumentar Americana é uma parasitose importante no município de Juína. Os dados sugerem relação com a atividade laboral e um perfil de transmissão predominantemente silvestre, o que deve ser levado em conta ao definir medidas de controle da doença na região

  11. Growth and cesium uptake responses of Phytolacca americana Linn. and Amaranthus cruentus L. grown on cesium contaminated soil to elevated CO2 or inoculation with a plant growth promoting rhizobacterium Burkholderia sp. D54, or in combination.

    Science.gov (United States)

    Tang, Shirong; Liao, Shangqiang; Guo, Junkang; Song, Zhengguo; Wang, Ruigang; Zhou, Xiaomin

    2011-12-30

    Growth and cesium uptake responses of plants to elevated CO(2) and microbial inoculation, alone or in combination, can be explored for clean-up of contaminated soils, and this induced phytoextraction may be better than the natural process. The present study used open-top chambers to investigate combined effects of Burkholderia sp. D54 inoculation and elevated CO(2) (860 μL L(-1)) on growth and Cs uptake by Phytolacca americana and Amaranthus cruentus grown on soil spiked with various levels of Cs (0-1000 mg kg(-1)). Elevated CO(2) and bacterial inoculation, alone or in combination, significantly increased biomass production with increased magnitude, ranging from 22% to 139% for P. americana, and 14% to 254% for A. cruentus. Total tissue Cs in both plants was significantly greater for bacterial inoculation treatment singly, and combined treatments of bacterial inoculation and elevated CO(2) than for the control treatment in most cases. Regardless of CO(2) concentrations and bacterial inoculation, A. cruentus had higher tissue Cs concentration, Cs transfer factors and concentration ratios than P. americana, but they had slightly different contents of antioxidant enzymes. It is concluded that combined effects of elevated CO(2) and microbial inoculation with regard to plant ability to grow and remove radionuclides from soil can be explored for CO(2)- and microbe-assisted phytoextraction technology. Copyright © 2011 Elsevier B.V. All rights reserved.

  12. Synthèses enzymatiques de néoglucoconjugués catalysées par l'alpha-glucosidase purifiée de la blatte Periplaneta americana (Linnaeus

    Directory of Open Access Journals (Sweden)

    Kamenan A.

    2005-01-01

    Full Text Available Enzymatic synthesis of neoglucoconjugates by purified α-glucosidase from cockroach Periplaneta americana (Linnaeus. Cockroach Periplaneta americana (Linnaeus contains in his digestive tract an acid (pH 5,0 and mesophile (50°C α-glucosidase. This enzyme, purified to homogeneity, hydrolyses highly maltose, sucrose and p-nitrophenyl-α-Dglucopyranoside. The ability of α-glucosidase from cockroach purified to homogeneity to catalyse transglucosylation reactions was tested using maltose and saccharose as glucosyl donors and 2-phenylethanol and phenol as acceptors. The experimental conditions were optimized in relation to the time course of the reaction, pH and concentrations of glucosyl donors and acceptors. The yields in transglucosylation reactions at 37 °C were very high and could attain 67% and 48% with 2-phenylethanol and phenol respectively as glucosyl acceptors. This α-glucosidase hydrolyzed the products formed. It seems that the products formed were the phenylethyl-α-D-glucoside and phenyl-α-D-glucoside. These results suggest that α- glucosidase from cockroach is an exoglucosidase which catalyse the splitting of the α-glucosyl residue from the non reducing terminal of the substrate to liberate α-glucose. This comportment indicates that this enzyme operated by a mechanism involving the retention of the anomeric configuration. On the basis of this work, α-glucosidase from P. americana appears to be a valuable tool for the preparation of α-neoglucoconjugates.

  13. Comparing the thermo-physical characteristics of lard and selected plant fats

    Directory of Open Access Journals (Sweden)

    Yanty, N. A. M.

    2012-09-01

    Full Text Available A comparison of the thermo-physical properties of lard and plant fats may help to formulate alternative fat substitutes for halal food applications. In this study, plant-based fats, namely avocado butter (Persea americana, cocoa butter (Theobroma cacao L., palm oil (Elaeis guinensis and mee fat (Madhuca longifolia are compared to lard with respect to the basic physico-chemical parameters, fatty acid and triacylglycerol (TAG compositions, and melting and solidification behaviors. Although plant fats are completely different from lard with respect to fatty acid and TAG compositions, they share some common thermal features with lard. Based on thermal analysis, lard and plant fats, except cocoa butter, are found to have thermal transitions in both low (< 0 °C and high (> 0 °C melting regions of their cooling and melting curves. According to pulse NMR data, mee fat and lard are found to display closely similar solidification profiles in the temperature range of 0-25 °C, while palm oil and lard are found to have similar solidification profiles in the temperature range between 25-40 °C. Hence, the thermo-physical property comparison between plant fats and lard may be useful to formulate a fat blend which simulates the thermal properties of lard.

    La comparación de las propiedades térmica y mecánicas de la manteca de cerdo y la de determinadas grasas de plantas, podría ayudar a formular sustitutos alternativos de las grasas para aplicaciones alimentarias. En este estudio, basado en materias grasas vegetales como, aguacate (Persea americana, manteca de cacao (Theobroma cacao L., palma aceitera (Elaeis guinensis y grasa de mee (Madhuca longifolia se comparan con la manteca de cerdo con respecto a parámetros físico-químicos, composiciones en ácidos grasos y triglicéridos (TAG, y comportamientos de los parámetros de fusión y de solidificación. Aunque las grasas de plantas

  14. Characterization of the oils from the pulp and seeds of avocado (cultivar: Fuerte fruits

    Directory of Open Access Journals (Sweden)

    Queiroz Paulo, Marçal

    2001-08-01

    Full Text Available Avocado (Persea americana Mill pulp and seeds of the cultivar Fuerte, cultivated in the Northeast region of Brazil, were analysed for their proximate composition. The lipid contents of the pulp and seeds were 15.39% and 1.87%, respectively. Hexane extracted oils from pulp and seeds presented similar refractive indices (1.4608 and 1.4592, specific gravity (0.9272 and 0.9300 and peroxide values (1.40 and 1.37, but different acid (2.45 and 4.12, iodine (77.6 and 69.4 and saponification values (178.3 and 231.6, respectively. Gas chromatographic analysis revealed the presence of twenty two and twenty seven fatty acids in pulp and seed oils, respectively. Palmitic acid was the principal saturated acid, present in concentrations of 21.3% in pulp and 20.8% in seed oils. The major difference between these oils was in their mono-unsaturated (C18:1 - 64.3%, for pulp and 15.4% in seed oil and poly-unsaturated (C18:2 and C18:3 - 9.14% and 0.46%, respectively, in pulp oil and 34.39% and 5.81%, respectively, in seed oil fatty acids composition.La semilla y pulpa del aguacate (Persea americana Mill, cultivar Fuerte, cultivado en la región del nordeste de Brasil fueron analizados para determinar su composición centesimal. El contenido de lípidos en pulpa y semillas, en la base húmeda fueron 15.39% y 1.87%, respectivamente. Los aceites extraídos de la pulpa y de las semillas por hexano representan similares índices de refracción (1.4608 y 1.4592, densidad específica (0.9272 y 0.9300 e índice de peróxidos (1.40 y 1.37 pero diferentes índices de acidez (2.45 y 4.12, iodo (77.6 y 69.4 y saponificación (178.3 y 231.6, respectivamente. El análisis de cromatografía gaseosa revela la presencia de 22 y 27 ácidos grasos en aceite de pulpa y de semillas, respectivamente. El ácido palmítico fue el principal ácido saturado, presente en concentraciones de 21,3% en pulpa y 20,8% en aceites de semillas. La principal diferencia entre estos aceites fue su

  15. Perfil de isotipos de imunoglobulinas e subclasses de IgG na leishmaniose tegumentar americana Immunoglobulin isotype and IgG subclass profiles in american tegumentary leishmaniasis

    Directory of Open Access Journals (Sweden)

    Maria Aparecida de Souza

    2005-04-01

    Full Text Available O presente trabalho avaliou o perfil de anticorpos em amostras de soro de 37 pacientes com diagnóstico clínico confirmado ou compatível com leishmaniose tegumentar americana atendidos no Hospital de Clínicas da Universidade Federal de Uberlândia, MG. Os perfis das classes de imunoglobulinas e subclasses de IgG foram analisados pelo teste ELISA indireto, utilizando-se antígeno solúvel de Leishmania (Leishmania amazonensis. A avidez dos anticorpos foi determinada pelo tratamento com uréia a 6 M, após incubação dos soros com o antígeno. Observou-se que 97%, 94,6%, 57,5 e 21,5% das amostras testadas apresentaram anticorpos anti-Leishmania das classes IgE, IgG, IgA e IgM, respectivamente e, os perfis das subclasses de IgG demonstraram, IgG1>IgG3>IgG2>IgG4. Os anticorpos IgE anti-Leishmania de alta avidez corresponderam a 44,4%. Por outro lado, IgG e IgA anti-Leishmania foram em sua maioria (62,8 e 47,8%, respectivamente, de média avidez. A variação do perfil de isotipos, bem como a avidez das imunoglobulinas refletiu a complexidade da resposta imune humoral contra a leishmaniose tegumentar americana.The present work investigated the serum antibody profiles in 37 patients with American tegumentary leishmaniasis, who were attended at Hospital de Clinicas - Universidade Federal de Uberlandia, MG, Brazil. The immunoglobulin class and IgG subclass profiles were analyzed by indirect ELISA using Leishmania (Leishmania amazonensis soluble antigen. The antibody avidity was determined by 6 M urea treatment after incubation with immunoenzymatic conjugate. It was observed that 97% of the serum samples presented anti-Leishmania antibodies for IgE class, 94.6% IgG, 57.5% IgA and 21.5% IgM class. For IgG subclasses the profiles were in the following order of frequency: IgG1>IgG3>IgG2>IgG4. High avidity of anti-Leishmania IgE antibodies was found in 44.4% of the samples. On the other hand, moderate avidity of specific IgG and IgA was observed in 62

  16. Contenido total de fenoles y actividad antioxidante de pulpa de seis frutas tropicales

    OpenAIRE

    Elizabeth Moreno; Blanca L. Ortiz; Luz P. Restrepo

    2014-01-01

    Seis pulpas de frutas tropicales de origen colombiano: curuba (Passiflora tripartida var. Mollissima), gulupa (Passiflora edulis Sims), aguacate variedad Hass (Persea America Mill), lulo (Solanum quitoense Lam.), tomate de árbol (Cyphomandra betacea Sendt) y uchuva (Physalis peruviana L) fueron empleadas para realizar el estudio del contenido total de fenoles, por el método de Folin Ciocalteu y la actividad antioxidante por los métodos químicos: DPPH (2,2-difenil-2-picrilhidrazilo) y FRAP (po...

  17. American foundations and the age of global philanthropy: how Brazil fares in the grant$ race = Fundações americanas e a era da filantropia global: como o Brasil está longe na corrida por fomento = Fundaciones americanas y la era de filantropía mundial: cómo va Brasil en la carrera de los subvenciones

    Directory of Open Access Journals (Sweden)

    Jasinski, Lisa

    2016-01-01

    Full Text Available Este artigo examina fundações privadas dos Estados Unidos que fazem doações às organizações não governamentais – ONGs e instituições educacionais no Brasil, durante e após a recessão global (2006-2012. Usando dados americanos, os autores identificam as fundações privadas americanas que destinam mais dinheiro para o Brasil, nomeiam as razões das instituições que recebem o maior apoio internacional e identificam como as fundações brasileiras têm sido impactadas pela crise financeira mundial. O artigo apresenta seções que descrevem a história do financiamento da educação, a literatura de pesquisas sobre filantropia global, a metodologia, os resultados e recomendações para estudiosos brasileiros da forma mais eficaz de concretizar subvenções

  18. Últimos avances médicos presentados ante la 93ª sesión anual de la Asociación Médica Americana en Atlantic city, Estados Unidos, junio 8 a junio 12 de 1942. Contribuciones latino-americanas

    Directory of Open Access Journals (Sweden)

    Benjamín Mera

    1942-08-01

    Full Text Available Una sinopsis de los hechos más notables presentados a la Asociación Médica Americana como fruto del trabajo de investigadores de diversas partes del Hemisferio Occidental, reviste la mayor importancia para la información de los profesionales colombianos. Datos generales, y en lo posible lo más concisos, se dan en este resumen sobre trabajos personalmente seleccionados de Medicina General, Patología, Fisiología, Medicina Tropical e Higiene Pública, los cuales fueron presentados o exhibidos ante los 8.103 médicos que asistieron a la Convención médica más importante de los tiempos actuales.

  19. Growth and cesium uptake responses of Phytolacca americana Linn. and Amaranthus cruentus L. grown on cesium contaminated soil to elevated CO{sub 2} or inoculation with a plant growth promoting rhizobacterium Burkholderia sp. D54, or in combination

    Energy Technology Data Exchange (ETDEWEB)

    Tang, Shirong, E-mail: tangshir@hotmail.com [Centre for Research in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191 (China); Key Laboratory of Production Environment and Agro-product Safety of Ministry of Agriculture, Tianjin (China); Liao, Shangqiang; Guo, Junkang; Song, Zhengguo; Wang, Ruigang [Centre for Research in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191 (China); Key Laboratory of Production Environment and Agro-product Safety of Ministry of Agriculture, Tianjin (China); Zhou, Xiaomin [Plant Science Department, McGill University, Macdonald Campus, 21111 Lakeshore Road, Ste. Anne de Bellevue, Quebec, Canada H9X 3V9 (Canada)

    2011-12-30

    Highlights: Black-Right-Pointing-Pointer Elevated CO{sub 2} and microbial inoculation, alone or in combination, significantly promoted growth of P. americana, and A. cruentus. Black-Right-Pointing-Pointer Total tissue Cs in plants was significantly increased. Black-Right-Pointing-Pointer A. cruentus had higher tissue Cs concentration, Cs transfer factors and concentration ratios than P. americana. Black-Right-Pointing-Pointer The two plants had slightly different contents of antioxidant enzymes. Black-Right-Pointing-Pointer Combined effects of elevated CO{sub 2} and microbial inoculation can be explored for CO{sub 2}- and microbe-assisted phytoextraction technology. - Abstract: Growth and cesium uptake responses of plants to elevated CO{sub 2} and microbial inoculation, alone or in combination, can be explored for clean-up of contaminated soils, and this induced phytoextraction may be better than the natural process. The present study used open-top chambers to investigate combined effects of Burkholderia sp. D54 inoculation and elevated CO{sub 2} (860 {mu}L L{sup -1}) on growth and Cs uptake by Phytolacca americana and Amaranthus cruentus grown on soil spiked with various levels of Cs (0-1000 mg kg{sup -1}). Elevated CO{sub 2} and bacterial inoculation, alone or in combination, significantly increased biomass production with increased magnitude, ranging from 22% to 139% for P. americana, and 14% to 254% for A. cruentus. Total tissue Cs in both plants was significantly greater for bacterial inoculation treatment singly, and combined treatments of bacterial inoculation and elevated CO{sub 2} than for the control treatment in most cases. Regardless of CO{sub 2} concentrations and bacterial inoculation, A. cruentus had higher tissue Cs concentration, Cs transfer factors and concentration ratios than P. americana, but they had slightly different contents of antioxidant enzymes. It is concluded that combined effects of elevated CO{sub 2} and microbial inoculation with

  20. Comparison of food habits of white perch (Morone americana) in the heated effluent canal of a steam electric station and in an adjacent river system

    International Nuclear Information System (INIS)

    Moore, C.J.; Fuller, S.L.H.; Burton, D.T.

    1975-01-01

    Analysis of the stomach contents of 97 white perch, Morone americana, taken from the effluent canal of a steam electric station (S.E.S.) and 106 white perch from adjacent Patuxent River waters indicated similar food habits from September 1970 through August 1971. However, 35 percent of all white perch taken from the heated effluent canal contained small pieces of coal and cinders, whereas only 3 percent of the river specimens contained such items in their stomachs. Fly ash and coal dust are present on the bottom of the S.E.S. canal, whereas little such material, if any, can be found on the river bottom in the study area. This suggests the canal fish were actively feeding in the heated effluent and not simply moving into the canal after feeding in the river. No significant difference (P greater than 0.05) was found between the average wet weight stomach contents of the river and canal fish within the same month