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Sample records for aegypti na ilha

  1. Fatores associados à ocorrência de formas imaturas de Aedes aegypti na Ilha do Governador, Rio de Janeiro, Brasil Factors associated to the ocurrence of immature forms of Aedes aegypti in the Ilha do Governador, Rio de Janeiro, Brazil

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    Reinaldo Souza-Santos

    1999-08-01

    Full Text Available Aedes aegypti é o vetor urbano da dengue, doença que pode resultar em epidemias. Estudos ecológicos tornam-se importantes uma vez que populações do vetor de diferentes áreas podem diferir quanto a características bio-ecológicas, relevantes para orientar ações de controle. Este trabalho objetiva identificar e analisar fatores associados à ocorrência de formas imaturas de A. aegypti na Ilha do Governador, Rio de Janeiro, a partir dos dados da Fundação Nacional de Saúde (FNS. Os resultados mostram que 58,04% do total de criadouros inspecionados foram constituídos por suportes para vasos com plantas, vasilhames de plástico ou vidro abandonados no peridomicílio. Maiores percentuais de criadouros positivos foram observados para pneus (1,41%, tanques, poços e cisternas (0,93%, e barris, tonéis e tinas (0,64%. Maiores proporções de criadouros positivos durante o verão foram as dos grandes reservatórios de água e a dos criadouros provenientes do lixo doméstico. No inverno, verificamos maior valor para os pequenos reservatórios de água para uso doméstico. As maiores proporções de criadouros positivos foram observadas após três meses sem atividades da FNS. A análise fatorial mostrou que o principal fator determinante da ocorrência de fases imaturas de A. aegypti é aquele que leva em consideração os fatores meteorológicos. A eliminação e tratamento de criadouros pelos agentes da FNS apresentaram-se como menos importantes. Tais fatos apontam a necessidade de controle contínuo, indicando menor atenção da FNS, durante o inverno, em relação aos pequenos reservatórios, que podem manter formas imaturas de A. aegypti.Aedes aegypti is the vector of dengue, a disease that can result in epidemics. Ecological studies are important because different geographical populations of the vector may differ in their bio-ecological characteristics, which can be helpful in guiding control actions. The objective of this study was to

  2. [The factors associated with the occurrence of immature forms of Aedes aegypti in Ilha do Governador, Rio de Janeiro, Brazil].

    Science.gov (United States)

    Souza-Santos, R

    1999-01-01

    Aedes aegypti is the vector of dengue, a disease that can result in epidemics. Ecological studies are important because different geographical populations of the vector may differ in their bioecological characteristics, which can be helpful in guiding control actions. The objective of this study was to identify and to analyze some factors associated with the occurrence of immature forms of A. aegypti on Ilha do Governador, Rio de Janeiro, using data from the National Health Foundation (FNS). The results showed that 58.04% of all the containers examined were supports for plant pots, and plastic or glass cans discarded in the yard. The highest percentages of positive breeding sites were observed in tires (1.41%), wells and cisterns (0.93%), and barrels and large metal cans (0.64%). In the summer, the proportion of positivity was higher for big containers used for water storage and for containers discarded in the yard. In the winter the proportion was higher for small containers used for water storage. The highest rates of positive containers were observed after three months without FNS activities. Our results show the necessity to continue the control, and suggest that less attention was paid to small containers during the winter by FNS. Factorial analysis showed that the determinant factor for the occurrence of immature forms of A. aegypti is represented by environmental factors, while elimination and treatment of breeding sites by FNS play a less important role, a fact possibly causing the maintenance of immature forms of A. aegypti.

  3. A água na Ilha da Madeira

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    João Manuel de Lemos Baptista

    2014-02-01

    Full Text Available Este artigo aborda a problemaÅLtica da aÅLgua na Madeira, desde a origem do povoamento ateÅL aos nossosdias, com especial atencao para a exploracao e salvaguarda deste recurso natural.Na Madeira, a aÅLgua existe em abunda^ncia, gracas aos fenoÅLmenos climaÅLticos que ocorrem na ilha, devidotambeÅLm ao relevo e a` natureza das rochas serem predominantemente basaÅLlticas (impermeaÅLveis etambeÅLm devido aos investimentos que o Governo Regional tem efetuado, nomeadamente a niÅLvel daexploracao, atraveÅLs da construcao de furos e de galerias para extrair a aÅLgua subterra^nea, como forma desatisfazer as necessidades da populacao no seu todo e dos turistas que nos visitam.Nao obstante, eÅL fundamental continuar com uma gestao equilibrada e sisteÅLmica da aÅLgua, para que nofuturo este liÅLquido continue nas nossas vidas. Felizmente que na Madeira, a empresa de capitaispuÅLblicos IGA – Investimentos e Gestao da AÅLgua, S.A. tem realizado um trabalho excelente em termosde exploracao, tratamento e abastecimentos a`s populacoes da Madeira e do Porto Santo, assim comouma gestao sustentaÅLvel deste recurso.This article discusses the problem of water in the wood, from the origin of the settlement to the presentday, with special attention to the exploitation and protection of this natural resource. In Madeira, waterexists in abundance, thanks to the weather phenomena that occur on the island, also due to relief andthe nature of the rocks are predominantly basaltic (waterproof and also due to the investments thatthe Regional Government has made, particularly in terms of the exploration, through the constructionof boreholes and galleries to extract groundwater as a way to meet the needs of the whole populationand the tourists who visit us.Nevertheless, it is essential to continue with a balanced and systematic management of water, so thatin future this fluid continues in our lives. Fortunately in Madeira, the company —owned IGA

  4. Sobre a ocorrência da Lutzomyia longipalpis (Lutz & Neiva, 1912 na Ilha Grande, Estado do Rio de Janeiro, Brasil

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    Nelson A. de Araújo Filho

    1980-12-01

    Full Text Available Os autores descrevem peta primeira vez a presença de Lutzomyia longipalpis na Praia Vermelha, praia localizada na Ilha Grande, litoral do Estado do Rio de Janeiro e acentuam a importância epidemiológica do achado.

  5. Observações biológicas e meteorológicas feitas na Ilha de Trindade

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    Rudolf Barth

    1958-07-01

    Full Text Available 1. As observações faunísticas, florísticas e climatológicas formam ainda um aspecto incompleto da Ilha de Trindade. Necessitamos ainda de observações em outras estações do ano e, também, do arquipélago de Martin Vaz, bem como de dados meteorológicos de alguns anos consecutivos. 2. A vegetação da ilha e, concomitantemente, a parte fértil da terra, estão desaparecendo pela influência de animais introduzidos (porcos, cabritos e carneiros que se alimentam das pucas plantas, destruindo o revestimento do solo e, assim, acelerando a erosão. Para possibilitar habitações permanentes em pequena escala, são necessárias duas operações imediatas; a diminuir ou, ainda melhor, eliminar por completo êstes animais cuja carne é comestível; b plantar árvores pelo menos na parte habitada e no córrego que abastece a guarnição com água. 3. O solo é rico em minerais, porém pobre em micro-organismos. Para horticultura basta uma adubação por estrume natural e cal, que existe em quantidade na ilha em forma de recifes coralinos. 4. É explicada a formação provàvel da inversão dos alísios e, com isso, o clima sêco da ilha. 5. De vários pontos de vista (ecologia, climatologia, de navegação e aviação, de previsão de tempo no continente, da pesca etc., torna-se aconselhável manter na ilha um pôsto permanente de meteorologia e radiossondagem.

  6. Hipertensão arterial na Ilha do Governador, Brasil: II. Prevalência

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    Klein Carlos Henrique

    1995-01-01

    Full Text Available Este segundo artigo (de uma série de dois tem como finalidade apresentar apenas os resultados básicos de um estudo, em amostra representativa de população, sobre Hipertensão Arterial na Ilha do Governador (região administrativa do município do Rio de Janeiro, em três estratos de acordo com a renda média domiciliar (baixa, média e alta de setores censitários. No conjunto da Ilha do Governador a prevalência de hipertensão não controlada foi de 16,1 % (I.C. de 95%: 13,4 a 18,9%, e incluindo-se os hipertensos controlados, este índice subiu para 24,9% (I.C. de 95%: 21,7 a 28,1 %. Por ambos os critérios (incluindo ou não os hipertensos controlados, as prevalências mais elevadas foram as do estrato de renda baixa e as menores do estrato de renda alta. Porém, só foi possível rejeitar a homogeneidade de prevalências de hipertensão (incluindo os controlados entre os estratos, e ainda assim para uma significância de 10%, quando se ajustou o efeito de confusão da idade. As mulheres hipertensas parecem controlar em maior proporção seus níveis de pressão arterial em qualquer faixa etária dos adultos, do que os homens.

  7. Sustainable agriculture in Ilha da Madeira - Portugal | Agricultura sustentável na Ilha da Madeira - Portugal

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    Raphael Miller de Souza Caldas

    2016-10-01

    Full Text Available Ilha da Madeira is an autonomous region of Portugal located in Atlantic Ocean, in center coordinates 32º43'N 17º43'O, is considered the largest island of archipelago of  the same name. The island covers an area of 740 km². The island has many climate peculiarities mainly influenced by altitude and barriers formed by sharp reliefs, and that they provide North / South axis. Climate variability existing of the island is defined in two types temperate in north and subtropical climate in south. The part of North is cooler and wetter than the south, due to winds from North and end up having a barrier relief inherent to region. Since the year of 1419, the island was subject to various economic and agricultural cycles. Actually, the agricultural occupation of the territory is predominantly winery, horticultural and fruit production. The study was conducted from January to May 2016. Data in census institutes and scientific articles were compared for discussion. The data of production, area of cultivation were intertwined with sustainable agriculture like organic farming and possibilities was available for openings boundaries according to adjustment of the culture temperature, photoperiod and precipitation, and may contribute to increase economy and quality of life in island. The agriculture of the island is formed basically by agriculture family subsistence, involving more than 45,000 people, representing 20% of Madeira population resulting only 3% of the region's economy.

  8. Surto epidêmico de sarampo na Ilha Grande, Rio de Janeiro, Brasil

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    Nelson A. Araújo Filho

    1980-12-01

    Full Text Available Os autores descrevem durante os meses de março e abril de 1976, a ocorrência de um surto de sarampo com 50 casos, em um povoado da Ilha Grande, Rio de Janeiro, situado a 17 km de distância do continente. A incidência sobre a população residente na área de 453 pessoas foi de 11,03%; na faixa entre um a 15 anos não ocorreram diferenças estatisticamentes significativas entre as taxas de incidência; nenhum caso acometeu menores de um ano, e três casos ocorreram em maiores de 15 anos; os autores acreditam que há muitos anos não ocorria sarampo na área, e que provavelmente daqui a cinco ou sete haverá novo surto; concluem também que apenas a imunização dos estudantes será suficiente para prevenir outros membros da comunidade de adquirirem sarampo, visto serem os estudantes responsáveis pela disseminação do surto através da escola primária da área.

  9. Hipertensão arterial na Ilha do Governador, Brasil: II. Prevalência Arterial hypertension in Ilha do Governador, Rio de Janeiro, Brazil: II. Prevalence

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    Carlos Henrique Klein

    1995-09-01

    Full Text Available Este segundo artigo (de uma série de dois tem como finalidade apresentar apenas os resultados básicos de um estudo, em amostra representativa de população, sobre Hipertensão Arterial na Ilha do Governador (região administrativa do município do Rio de Janeiro, em três estratos de acordo com a renda média domiciliar (baixa, média e alta de setores censitários. No conjunto da Ilha do Governador a prevalência de hipertensão não controlada foi de 16,1 % (I.C. de 95%: 13,4 a 18,9%, e incluindo-se os hipertensos controlados, este índice subiu para 24,9% (I.C. de 95%: 21,7 a 28,1 %. Por ambos os critérios (incluindo ou não os hipertensos controlados, as prevalências mais elevadas foram as do estrato de renda baixa e as menores do estrato de renda alta. Porém, só foi possível rejeitar a homogeneidade de prevalências de hipertensão (incluindo os controlados entre os estratos, e ainda assim para uma significância de 10%, quando se ajustou o efeito de confusão da idade. As mulheres hipertensas parecem controlar em maior proporção seus níveis de pressão arterial em qualquer faixa etária dos adultos, do que os homens.The aim of this paper (the second of two is to present the basic results of a cross–sectional study on arterial hypertension in adults in Ilha do Governador (a district of the city of Rio de Janeiro, Brazil, with a representative sample of the population, stratified by the census tracts' mean household incorre (low, median, and high. Overall, prevalence of uncontrolled hypertension in Ilha do Governador was 16.1% (C.I. of 95%: 13.4 to 18.9%, and with the inclusion of controlled hypertensive individuals this figure rose to 24.9% (C I. of 95%: 21.7 to 28.7%. Under both criteria (whether including controlled hypertensive individuais or not, higher prevalences were from low‑incorre strata, while lower ones were from the high‑incorre strata. However, it was only possible to reject the hypothesis of strata homogeneity

  10. Contribuição ao estudo do mal de cadeiras na Ilha de Marajó

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    Geth Jansen

    1941-01-01

    Full Text Available Como resultado de inspeções feitas à ilha de Marajó o autor assinala: 1º O mal de cadeiras continua existindo na ilha de Marajó sob a forma enzoótica, ao contrário do que pensavam os criadores, julgando-o extinto. As doenças designadas por estes como "barriga inchada" e "mormo seco", responsaveis pela grande mortalidade dos equinos, foram identificadas pelo autor como tal de cadeiras. 2º O autor considera indispensável para a pesquisa do trypanosoma o processo de inoculação em animais de laboratório, tendo utilizado para esse fim camondongos e ratos brancos. 3º Os surtos epizoóticos anuais coincidem com a época das chuvas, ocasião em que surgem os insetos hematófagos, o que faz pensar no papel desses últimos como transmissores. 4º Os cavalos podem atravessar o ano com trypanosomas no sangue e em estado de saude aparente, constituindo assim depositários do mal de cadeiras. 5º O extermínio da capivara, em consequência do seu aproveitamento econômico não modificou o carater endêmico da doença na ilha de Marajó. 6º O autor observou que os trypanosomas no tubo digestivo de Stomoxys e Tabanideos, ao cabo de 8 horas, apresentavam com movimentos diminuidos e em formas arrendondadas, não conseguindo inoculações positivas com esse material. 7º A saliva de um morcego do gênero Desmodus, que se infectou no laboratório, alimentando-se em animal doente, colhida de modo a evitar lesões da mucosa e consequente contaminação pelos trypanosomas do sangue circulante, mostrou-se incapaz de infectar, por inoculação, animais sensiveis. Este mesmo morcego não transmitiu a doença a um cão, o qual sugou durante 80 dias. Recentemente (1941 empregando um exemplar do gênero Desmodus, também infectado experimentalmente por alimentação em animal de laboratório, conseguimos transmitir o Trypanosoma equinum a uma cobaia normal. Este assunto será ventilado posteriormente.

  11. mineração reabilitada na ilha da madeira, Itaguaí, RJ

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    Marcos Paulo Dos Santos Pereira

    2007-01-01

    Full Text Available Ant species richness and diversity may be increased in more complex habitats because they provide more niches and diverse ways of exploiting the environmental resources. Because of the connection between the organisms and habitat characteristics, ants have been used as tools for environmental monitoring of disturbed areas. In this study the structure of the ant community was investigated in plots with different management strategies for the rehabilitation of a mined area at Ilha da Madeira, Itaguaí, RJ, Brazil. A different and richer ant fauna was found in plots with more native tree species than in plots with a low number of tree species or without conservationist management.

  12. Fungos filamentosos do solo da Ilha dos Eucaliptos na Represa do Guarapiranga em São Paulo, SP

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    Santos,Valéria Barros dos; Wellbaum,Christian; Schoenlein-Crusius,Iracema Helena

    1998-01-01

    Foi estudada a diversidade dos fungos do solo da Ilha dos Eucaliptos na Represa do Guarapiranga em São Paulo, SP, sob floresta de Eucalyptus, mata de interseção e mata secundária remanescente, em duas épocas climáticamente distintas: março - época quente e úmida (verão) e junho - época fria e seca (inverno) de 1996. Os fungos filamentosos foram isolados pelos métodos da diluição em série e placa de solo. A similaridade entre as micotas foi estabelecida pelo índice de Sörensen. No total foram ...

  13. Scorpionism due to Tityus neoespartanus (Scorpiones, Buthidae in Margarita Island, northeastern Venezuela Escorpionismo por Tityus neoespartanus (Scorpiones, Buthidae na Ilha de Margarita, Nordeste da Venezuela

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    Leonardo De Sousa

    2007-12-01

    Full Text Available The first two cases of scorpion envenoming caused by Tityus neoespartanus (Buthidae are described. The accidents took place within human environments (one inside a home and the other inside a school, in the village of La Sierra, Margarita Island, State of Nueva Esparta, northeastern Venezuela. Both cases were moderately severe and developed pancreatic involvement and electrocardiographic abnormalities. This report allows inclusion of Margarita Island among the endemic areas of scorpionism in Venezuela.Descrevem se os dois primeiros casos de escorpionismo por Tityus neoespartanus (Scorpiones, Buthidae. Os acidentes ocorreram no ambiente antrópico (um no domicilio e outro na escola na aldeia de La Sierra, Ilha de Margarita, Estado de Nueva Esparta, nordeste da Venezuela. Os dois acidentes, moderadamente graves, cursaram com acometimento pancreático e alterações eletrocardiografias. Este reporte permite incluir à Ilha de Margarita entre as áreas endêmicas de escorpionismo na Venezuela.

  14. Epidemiologia de um caso de doença de Chagas na Ilha do Mosqueiro - Pará

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    Adelson A.A. de Souza

    1988-12-01

    Full Text Available Os autores apresentam os resultados do estudo epidemiológico de um caso autóctone da fase aguda da doença de Chagas na ilha do Mosqueiro, Estado do Pará, aproximadamente 75km da capital, Belém. 0 caso já havia sido objeto de uma publicação anterior. Agora são apresentadas informações epidemiológicas. Nas proximidades da casa do paciente foram capturados em duas palmeiras de Inajá ('Maximilian regi ay e em uma de Mucajá (Acrocomia sclerocarpia 114 triatomíneos: Rhodnius pictipes, R. robustus, Panstrongylus lignarius, P. geniculatus e Microtriatoma trinidadensis, com tripanossomas em 31 deles. Na casa do paciente foram encontrados exemplares de Rhodnius pictipes, infectados com formas metacíclicas do Trypanosoma cruzi. Em 14 marsupiais, capturados na localidade, haviam 3 infectados com organismos semelhantes ao T. cruzi. A eletroforese dos isoenzimas nos tripanossomas isolados do paciente, de R. pictipes e de Didelphis marsupialis os classificou como zimodema 1. Os autores concluem que a doença de Chagas do paciente teve origem silvestre.

  15. Leishmaniose tegumentar americana na Ilha Grande. Rio de Janeiro: V. Observações sobre a biologia dos transmissores em condiçoes naturais

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    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Durante um surto de Leishmaniose Tegumentar Americana (LTA na Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, foram coletados 4.192 exemplares de flebotomíneos em ambiente domiciliar e silvestre, com isca animal e outros abrigos naturais, durante o período de janeiro de 1976 a abril de 1977. Entre o total de 11 espécies coletadas encontraram-se 2.493 exemplares de Lutzomyia intermedia, 1.594 de Lutzomyia migonei e 105 exemplares de outras espécies. A L. intermedia apresentou densidade mais elevada no domicílio humano, enquanto a L. migonei apresentou coletas mais rendosas com iscas animais e galinheiros. O sinantropismo e a elevada densidade no ambiente domiciliar incriminam as espécies L. intermedia e L. migonei como as prováveis vetoras da LTA na Ilha Grande.

  16. As Práticas Turísticas na Orla Oeste da Ilha de Mosqueiro, Região Metropolitana de Belém, PA

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    Willame de Oliveira Ribeiro

    2013-10-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE MicrosoftInternetExplorer4 A Ilha do Mosqueiro pertence ao município de Belém, mas localiza-se a 77 km da sua sede. A Ilha teve seu processo de valorização para o Turismo iniciado no período áureo da economia da borracha e se acelerou com a implantação da ponte ligando-a ao continente, durante a década de 1970. Este artigo objetiva apresentar as práticas turísticas na orla oeste da Ilha de Mosqueiro, identificando o processo de des-re-territorialização de tais práticas e o viés do [eco] turismo como uma possibilidade de nova significação ao uso da orla oeste da ilha.  Tourist Practices in Waterfront West Island Mosqueiro, metropolitan region of Belém, PA, Brazil - The island Mosqueiro belonging to the municipality of Belem and it is located 77 km from the capital. The process for introduction of tourism practice started in the heyday of the rubber economy and it was accelerated during the 1970s with the build of the bridge connecting the island to the mainland. This article presents the tourism practices on the west of the Ilha do Mosqueiro, identifying the its process of de-re- territorialization and the [eco] tourism as a possibility for new meaning to the use of the western edge of the island.  

  17. USO DE GEOTECNOLOGIAS PARA AVALIAÇÃO DE MUDANÇAS NA COBERTURA VEGETAL DO ENTORNO DA ILHA DA LUZ

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    Caroline Tavares Firmino

    2017-04-01

    Full Text Available Os impactos decorrentes da ação antrópica podem ser vistos no solo, nas águas, na flora e na fauna. Como meio de monitorar esses impactos tem sido utilizada técnicas por meio do geoprocessamento, as quais são de suma importância, por fornecerem a possibilidade de monitorar as questões ambientais através de imagens obtidas via sensores orbitais. Objetivou-se analisar as mudanças ocorridas na formação vegetacional do entorno da Ilha da Luz em Cachoeiro de Itapemirim a partir da apreciação de duas imagens do Google Earth. Para a realização da análise temporal as imagens orbitais selecionadas foram importadas do Google Earth para o Autocad, onde foram realizadas as demarcações e medições das formações vegetacionais do entorno da Ilha. Constatou-se um aumento de 1127.0002m² da área vegetacional da Ilha da Luz entre as duas imagens, decorrente da agregação de fragmentos, demonstrando que as ferramentas computacionais são relevantes para avaliação de impactos ambientais.

  18. Leishmaniose tegumentar americana na Ilha Grande. Rio de Janeiro: V. Observações sobre a biologia dos transmissores em condiçoes naturais

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    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Durante um surto de Leishmaniose Tegumentar Americana (LTA na Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, foram coletados 4.192 exemplares de flebotomíneos em ambiente domiciliar e silvestre, com isca animal e outros abrigos naturais, durante o período de janeiro de 1976 a abril de 1977. Entre o total de 11 espécies coletadas encontraram-se 2.493 exemplares de Lutzomyia intermedia, 1.594 de Lutzomyia migonei e 105 exemplares de outras espécies. A L. intermedia apresentou densidade mais elevada no domicílio humano, enquanto a L. migonei apresentou coletas mais rendosas com iscas animais e galinheiros. O sinantropismo e a elevada densidade no ambiente domiciliar incriminam as espécies L. intermedia e L. migonei como as prováveis vetoras da LTA na Ilha Grande.During an outbreak of mucocutaneous leishmaniasis in Praia Vermelha, Ilha Grande (Great Island in the county of Angra dos Reis, State of Rio de Janeiro, 4.192 species of Phlebotomus were collected in domestic and wild environment with the use of animal baits and other natural shelters from January 1976 to April 1977. In a total of 11 species collected, 2.493 samples of Lutzomyia intermedia, 1.594 of Lutzomyia migonei and 105 samples of other species were found. The Lutzomyia intermedia presented a higher density in human dwellings, while the Lutzomyia migonei presented better results with animal baits and chicken-coops. The sinantropism and high density found in the houses incriminate the species L. intermedia and L. migonei as the probable vectors of Mucocutaneous Leishmaniasis in Ilha Grande.

  19. Candomblé(s e espaço público na Ilha de Itaparica, Bahia

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    Fátima Tavares

    2015-12-01

    Full Text Available Resumo Neste artigo tomamos os conceitos de religião e espaço público como domínios relacionais. A partir dessa abordagem, seguimos as propostas de van de Port no que se refere à circularidade do candomblé de orixá no espaço público baiano, buscando articular as ideias do autor ao reconhecimento de que a circularidade produz uma cartografia atravessada por mediadores heterogêneos, ou seja, de que o candomblé propicia agenciamentos variados, incluindo-se, entre esses, os bens simbólicos de ampla circulação no espaço público. Com base nesses argumentos, procuramos compreender as relações entre candomblé de egun e candomblé de orixá na Ilha de Itaparica, apresentando elementos etnográficos que sugerem uma problematização do argumento de van de Port, indicando que a circulação do candomblé nessa localidade não se processa como em Salvador, mas apresenta assimetrias importantes nos modos de legitimação e visibilização social dessa religião em Itaparica.

  20. Leishmaniose tegumentar americana na Ilha Grande, Rio de Janeiro: I. investigação epidemiolôgica clínica e laboratorial

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    Nelson A. Araújo Filho

    1981-12-01

    Full Text Available Os autores descrevem pela primeira vez a ocorrência de uma epidemia de Leishmaniose Tegumentar Americana (LTAI na localidade de Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, situada a 17 Km do continente. Em uma população de 453 pessoas examinadas, foram encontrados 33 casos de LTA, sendo 13 lesões em atividade e 20 cicatrizes. Entre os 33 casos, 30 eram autóctones da área de estudo. A doença ocorria há mais de 10 anos na Praia Vermelha, e, na Ilha Grande, há mais de 30 anos, sob a forma de casos esporádicos. Na Praia Vermelha, no período de 1974 a 1976, ocorreu uma epidemia com 27 casos da doença. A L TA, na Praia Vermelha, assume características de transmissão em ambiente domiciliar, atingindo indivíduos indistintamente, sem relação com sexo, idade ou profissão. Predominou a forma ulcerada, em extremidades do corpo com ocorrência de cura espontânea das lesões e não comprometimento das mucosas; um hamster entre 10 inoculados com material de lesão de caso humano desenvolveu, após 4 meses, lesão de focinho com resultados positivos para LTA.

  1. Leishmaniose tegumentar americana na Ilha Grande, Rio de Janeiro: I. investigação epidemiolôgica clínica e laboratorial

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    Nelson A. Araújo Filho

    1981-12-01

    Full Text Available Os autores descrevem pela primeira vez a ocorrência de uma epidemia de Leishmaniose Tegumentar Americana (LTAI na localidade de Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, situada a 17 Km do continente. Em uma população de 453 pessoas examinadas, foram encontrados 33 casos de LTA, sendo 13 lesões em atividade e 20 cicatrizes. Entre os 33 casos, 30 eram autóctones da área de estudo. A doença ocorria há mais de 10 anos na Praia Vermelha, e, na Ilha Grande, há mais de 30 anos, sob a forma de casos esporádicos. Na Praia Vermelha, no período de 1974 a 1976, ocorreu uma epidemia com 27 casos da doença. A L TA, na Praia Vermelha, assume características de transmissão em ambiente domiciliar, atingindo indivíduos indistintamente, sem relação com sexo, idade ou profissão. Predominou a forma ulcerada, em extremidades do corpo com ocorrência de cura espontânea das lesões e não comprometimento das mucosas; um hamster entre 10 inoculados com material de lesão de caso humano desenvolveu, após 4 meses, lesão de focinho com resultados positivos para LTA.The authors described for the first time the appearance of an epidemic of Cutaneous Leishmaniasis in the small village of Praia Vermelha, Ilha Grande (Creat Island, county of Angra dos Reis, State of Rio de Janeiro, situated at 17 Km from the continent. 453 persons were examined and 33 cases of Cutaneous Leishmaniasis were found of which 13 were cases of active iesions and 20 of scars. Of the 33 cases, 30 were issued from the area under examination. The disease has existed in Praia Vermelha for more than 10 years and in the Ilha Grande for more than 30 as sporadic cases. In Praia Vermelha between 1974 and 1976, an epidemic occured when 27 cases of the disease were registered. The Cutaneous Leishmaniasis in Praia Vermelha has characteristics of home transmission affecting people without discrimination of sex, age or occupation. The ulcerated form appeared

  2. Estudo da Ilha de Calor na Região Metropolitana do Rio de Janeiro Usando Dados do MODIS

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    Caio Atila Pereira Sena

    2014-07-01

    Full Text Available A Região Metropolitana do Rio de Janeiro (RMRJ apresenta uma série de problemas ambientais, dentre eles, a ocorrência de Ilhas de Calor Urbanas (ICU e este trabalho tem como objetivo examinar as características da ICU na RMRJ. Para tal, foram usadas composições de 8 dias de dados de Temperatura Superficial Continental (TSC geradas pelo sensor MODIS (Moderate-resolution Imaging Spectroradiometer a bordo dos satélites Aqua e Terra no período de janeiro de 2003 a dezembro de 2010. A análise foi feita agrupando-se os dados para as diferentes estações do ano e posteriormente calculando-se a média de TSC para cada estação com o intuito de verificar a variação sazonal da ICU. Observou-se que, algumas zonas apresentaram temperaturas próximas dos 50°C durante a primavera e o verão. Ao longo do outono e do inverno as médias atingiram 40°C sobre a maior parte da região de estudo. Foram registradas amplitudes térmicas de até 25°C entre áreas com diferentes níveis de urbanização, durante a primavera. No verão a amplitude foi de 20°C entre as mesmas áreas. No outono a diferença foi de 15°C e no inverno, de 10°C.

  3. FAUNA DE FORMIGAS COMO FERRAMENTA PARA MONITORAMENTO DE ÁREA DE MINERAÇÃO REABILITADA NA ILHA DA MADEIRA, ITAGUAÍ, RJ

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    Pereira, Marcos Paulo dos Santos; Queiroz, Jarbas Marçal; Valcarcel, Ricardo; Mayhé-Nunes, Antônio José

    2007-01-01

    RESUMO A riqueza e diversidade de espécies de formigas podem ser maiores em ambientes de complexidade mais elevada devido a uma maior disponibilidade de nichos presentes. Em razão da conexão entre os organismos e as características dos habitats, as formigas têm sido utilizadas como ferramentas no monitoramento ambiental de áreas perturbadas. Neste estudo, foi investigada a estrutura da comunidade de formigas em parcelas utilizadas para reabilitação de uma área de mineração na Ilha da Madeira,...

  4. Epidemiologia de um caso de doença de Chagas na Ilha do Mosqueiro - Pará

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    Adelson A.A. de Souza

    1988-12-01

    Full Text Available Os autores apresentam os resultados do estudo epidemiológico de um caso autóctone da fase aguda da doença de Chagas na ilha do Mosqueiro, Estado do Pará, aproximadamente 75km da capital, Belém. 0 caso já havia sido objeto de uma publicação anterior. Agora são apresentadas informações epidemiológicas. Nas proximidades da casa do paciente foram capturados em duas palmeiras de Inajá ('Maximilian regi ay e em uma de Mucajá (Acrocomia sclerocarpia 114 triatomíneos: Rhodnius pictipes, R. robustus, Panstrongylus lignarius, P. geniculatus e Microtriatoma trinidadensis, com tripanossomas em 31 deles. Na casa do paciente foram encontrados exemplares de Rhodnius pictipes, infectados com formas metacíclicas do Trypanosoma cruzi. Em 14 marsupiais, capturados na localidade, haviam 3 infectados com organismos semelhantes ao T. cruzi. A eletroforese dos isoenzimas nos tripanossomas isolados do paciente, de R. pictipes e de Didelphis marsupialis os classificou como zimodema 1. Os autores concluem que a doença de Chagas do paciente teve origem silvestre.The authors present the results of an epidemiological study relating to a case of acute Chagas' disease acquired in the island of Mosqueiro, State of Para, approximately 75 km from the capital Belem. The patient has been the object of a previous publication but now epidemiological information is reported. Near the house of the patient in two Inaja palm trees (Maximilian regia and one Mucaja palm (Acrocomia sclerocarpia 114 triatomine bugs were captured of the following species: Rhodnius pictipes, R. robustus, Panstrongylus lignarius, P. geniculatus and Microtriatoma trinidadensis. Trypanosomes were found in 31 bugs. In the house of the patient specimens of R. pictipes were captured infected with metacyclic forms óf Trypanosoma cruzi. In 14 marsupials captured in the locality three had infections with cruzi like trypanosomes. Enzyme electrophoresis of the trypanosomes isolated from the patient, R

  5. Dinâmica Glacial e Características Sedimentares Resultantes na Zona Proglacial da Geleira Ecology-Baía do Almirantado, Ilha Rei George Antártica.

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    Kátia Kellem Rosa; Rosemary Vieira; Jefferson Cardia Simões

    2006-01-01

    A geleira Ecology, localizada na ilha Rei George, ilhas Shetlands do Sul, está sofrendo um processo de rápida retração ao longo das últimas décadas, sendo que no período de 1956 a 1992/95 a geleira retrocedeu 0,37 Km2. Isto gerou um ambiente de deglaciação, com a exposição de várias geoformas na zona proglacial. Este trabalho objetiva estudar o ambiente de deglaciação da zona proglacial da geleir...

  6. Caracterização hidráulica, hidrológica e de transporte sólido do evento de 20 de fevereiro de 2010 na Ribeira de João Gomes - Ilha da Madeira

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    Castro, João Nélio Remêsso

    2011-01-01

    O presente trabalho surge em resultado do evento de 20 de Fevereiro de 2010, com o objectivo de caracterizar a bacia hidrográfica da Ribeira de João Gomes, nas suas vertentes hidráulicas, hidrológicas e de transporte sólido. Inicialmente este trabalho relata as condições climáticas, geológicas e hidrológicas da ilha da Madeira, inclusive nos recursos hídricos e nas situações hidrológicas extremas. Em seguida é apresentado uma breve cronologia dos eventos aluviais presentes na ilha da Madei...

  7. Fungos filamentosos do solo da Ilha dos Eucaliptos na Represa do Guarapiranga em São Paulo, SP Filamentous fungi from the soil of the "Ilha dos Eucaliptos" in the "Represa do Guarapiranga" in São Paulo State, SP

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    Valéria Barros dos Santos

    1998-04-01

    Full Text Available Foi estudada a diversidade dos fungos do solo da Ilha dos Eucaliptos na Represa do Guarapiranga em São Paulo, SP, sob floresta de Eucalyptus, mata de interseção e mata secundária remanescente, em duas épocas climáticamente distintas: março - época quente e úmida (verão e junho - época fria e seca (inverno de 1996. Os fungos filamentosos foram isolados pelos métodos da diluição em série e placa de solo. A similaridade entre as micotas foi estabelecida pelo índice de Sörensen. No total foram obtidos 44 táxons de fungos (Zygomycota, Ascomycota e fungos mitospóricos, distribuídos em 92 ocorrências. No solo sob a floresta de Eucalyptus spp. foram encontrados 20 táxons (31 ocorrências de fungos, no solo da interseção 32 táxons (38 ocorrências e no solo da mata secundária 16 táxons (23 ocorrências. Similaridade de 47% foi encontrada entre a micota do solo sob Eucalyptus com a da interseção, e desta com a micota da mata secundária. A micota do solo sob Eucalyptus e a da mata secundária apresentaram 39% de similaridade. Na época quente e úmida (verão a micota terrestre da Ilha foi composta por 26 táxons de fungos (47 ocorrências e na época fria e seca (inverno por 32 táxons (45 ocorrências, com 46% de similaridade entre ambas. Pela diluição em série foram isolados 33 táxons (60 ocorrências e pela técnica da placa de solo, 22 táxons (32 ocorrências. O número de colônias de fungos foi significativamente maior no solo da mata secundária, seguido pela vegetação de interseção e a floresta de Eucalyptus, independentemente da época de coleta. O possível efeito inibitório de Eucalyptus sobre a comunidade dos fungos do solo da Ilha pode ter sido expresso pela diminuição do número de colônias na área reflorestada, no entanto, não afetou a diversidade dos fungos, que se apresentou elevada nos três tipos de vegetação.The aim of the present study was to compare the diversity of the fungi in the soil

  8. As espécies exóticas somos nós: reflexão a propósito do ecoturismo na Ilha Grande

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    Prado Rosane Manhães

    2003-01-01

    Full Text Available Este trabalho analisa as implicações da introdução do turismo na Ilha Grande (Angra dos Reis, RJ, em especial para a população da Vila do Abraão, que foi abruptamente transformada num local de grande procura por parte de turistas e veranistas. Outros casos já estudados indicam um modelo das conseqüências da introdução do turismo em contextos nos quais ele antes não existia, o que é ilustrado com o caso do Abraão. Particularmente a questão da polaridade entre "nativos" e "não-nativos" aflora ali em razão da intensificação do turismo, correspondendo a uma barreira na aceitação de uma ideologia ambientalista e de propostas generalizantes para o que seria "um desenvolvimento turístico ideal".

  9. DISTRIBUIÇÃO E FRACIONAMENTO DO FÓSFORO NOS SEDIMENTOS DOS RIOS BACANGA E ANIL NA ILHA DO MARANHÃO

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    MARIA DE LOURDES FERREIRA NUNES

    2013-01-01

    No presente estudo, foi determinado o estoque total de fósforo (TP) em sedimentos de rios Anil e Bacanga localizados na ilha do Maranhão. Além disso, com a finalidade de avaliar a labilidade de fósforo no sedimento, foi realizada a extração química sequencial de acordo com o protocolo da Comissão Europeia (normas, medidas e programa de testes). Os resultados do Rio Bacanga indicou concentrações de PT que variam de 877,91 mg kgˉ e 1466,44 mg Kgˉ, o que permite classificar estes sedim...

  10. Fungos micorrízicos arbusculares (Glomales, Zygomycota na Ilha dos Eucaliptos, Represa do Guarapiranga, São Paulo, SP Arbuscular mycorrhizal fungi (Glomales, Zygomycota from "Ilha dos Eucaliptos", Guarapiranga Reservoir, São Paulo, SP, Brazil

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    Simone de Paula Gomes

    1998-01-01

    Full Text Available Com o objetivo de se conhecer a micota de fungos micorrízicos arbusculares (FMA, Glomales, Zygomycota, coletou-se amostras de solo rizosférico de plantas instaladas em três áreas (plantio de eucaliptos, mata e transição entre ambas em três épocas (março/1996, época chuvosa e final de verão; junho/1996, época de inverno; janeiro/1997, início das chuvas e do verão na Ilha dos Eucaliptos (cerca de 323.500m², na Represa do Guarapiranga, São Paulo, SP. O solo foi processado para a separação dos esporos de FMA segundo a técnica do peneiramento e decantação em via úmida, seguindo-se centrifugação em solução de sacarose 50% (V:V. Os esporos isolados foram preparados em lâminas permanentes com resina PVL. Foram constatados 21 táxons de FMA: Acaulospora excávala Ingleby, Walker & Mason, Acaulospora foveata Trappe & Janos, Acaulospora longula Spain & Schenck, Acaulospora mellea Spain & Schenck, Acaulospora morrowiae Spain & Schenck, Acaulospora spinosa Walker & Trappe, Acaulospora sp. 1, Acaulospora sp. 2, Acaulospora sp. 3, Acaulospora sp. 4, Entrophospora colombiana Spain & Schenck, Gigaspora gigantea Nicol. & Gerd., Gigaspora ramisporopfwra Spain, Sieverding & Schenck, Glomus aggregatum (Schenck & Smith Koske, Glomus etunicatum Becker & Gerd., Glomus geosporum (Nicol. & Gerd. Walker, Glomus invennayum Hall, Glomus macrocarpum Tul. & Tui., Glomus multisubstensum Mukerjii, Bhattacharjee & Tewari, Scutellospora aff. biornata Spain, Sieverding & Toro e Scutellospora erythropa Koske & Walker. A aplicação do índice de similaridade de Sõrensen entre populações de FMA nas diferentes áreas e as épocas de coletas mostrou valores de 64 a 90%, sugerindo não haver seleção dos FMA mediada por esses fatores.With the aim of verifying the occurence of arbuscular mycorrhizal fungi AMF, Glomales, Zygomycota, rhizosphere soil samples were collected in three areas (eucaliptus reforestation, forest and intersection and on three

  11. Topossequência de solos na ilha da trindade, atlântico sul Topossequence of soils on the trindade island in the brazilian south atlantic

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    Eliane de Paula Clemente

    2009-10-01

    Full Text Available As ilhas oceânicas, como ambientes sui generis no planeta, têm despertado, cada vez mais, o interesse da comunidade científica em virtude de sua importância ambiental. Este trabalho teve por objetivo estudar os principais solos de ocorrência da Ilha da Trindade, enfatizando suas características químicas e físicas nos diferentes estratos ambientais de ocorrência. Buscou-se melhor entendimento das relações pedogeomorfológicas, permitindo um esboço preliminar da identificação dos solos ao longo da expressiva variação topográfica reinante na área de estudo. Ao longo de uma topossequência, foram coletados 10 perfis representativos dos diferentes pedoambientes, resultantes de variações litológicas, topográficas e de cobertura vegetal, muitas das quais covariantes. Foram realizadas análises físicas e químicas de todos os horizontes dos perfis coletados. A diversidade de solos na Ilha da Trindade pode ser relacionada com as variações do material de origem e da posição topográfica. De maneira geral, os solos apresentam peculiaridades que os tornam "endêmicos". A maioria possui alta fertilidade natural, grau de intemperismo pouco acentuado e valores muito elevados de P e Ca que parecem relacionados com atividade da avifauna. Na face sul da ilha, mais fria e úmida, vales estreitos e encostas íngremes abrigam vegetação mais exuberante de samambaias gigantes, com acúmulo em ambiente escarpado, de matéria orgânica fíbrica mesmo em declives acentuados, formando Organossolos em ambientes atípicos. Os solos de locais com altitude superior a 400 m são mais ácidos e pobres em nutrientes, mas com teor de P muito elevado em função de aportes da avifauna. Na face norte da ilha, em cotas mais baixas, predominam condições semiáridas ou tropicais secas, e os solos são mais rasos, ricos em nutrientes e muito mais erodidos, com predomínio de Neossolos Litólicos e Regolíticos. Há diversas particularidades nos solos da

  12. Pescadoras de camarão: gênero, mobilização e sustentabilidade na ilha Trambioca, Barcarena, Pará

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    Ligia Terezinha Lopes Simonian

    Full Text Available A partir deste artigo discute-se a relação entre gênero, mobilizações sociais e sustentabilidade no contexto da ilha Trambioca, município de Barcarena, Pará, especialmente quanto à experiência das pescadoras de camarão (Macrobrachium amazonicum. A pesquisa foi orientada pela metodologia antropológica do trabalho de campo e pela produção fotográfica, mas foram igualmente incorporadas estratégias que permitiram a produção de dados para uma análise quanto à economia política. Fundamentalmente, a mesma revelou uma experiência em que as mulheres pescadoras acabam sendo o destaque na produção de camarão nessa ilha, participando de todo o processo de produção, beneficiamento e comercialização. Em face à crise ambiental e econômica globalizada, tais pescadoras propõem a criação de camarão, com o que esperam aumentar a renda. Entretanto, nesse processo organizativo, elas percebem que os limites são muitos e que precisam de financiamento, capacitação e tecnologia voltadas para a criação de camarão. Conseqüentemente, muitas implicações apresentam-se para a sustentabilidade, em especial se a possibilidade de uma economia diferenciada for levada em consideração.

  13. Malhas da reciprocidade: a pesca coletiva da tainha na Ilha do Mel – litoral do Paraná

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    Evandro Cardoso do Nascimento

    2017-04-01

    Full Text Available The paper presents an analysis of the collective mullet fishing in Ilha do Mel Island from the theory of reciprocity in Temple (2003 and Sabourin (2009; 2011a; 2011b, as a basis for the observation of an empirical case in the coast of Paraná. The aim is to highlight the practices, relationships and reciprocity structures among fishermen by analyzing its implications for a more sustainable local/territorial development. It was found that even though relations of reciprocity and exchange coexist, there is a predominance of social and economic relations marked by the logic of reciprocity through collective fishing and these relationships contribute to material subsistence and social reproduction of these fishermen.

  14. Rudolf Barth: um cientista pioneiro na ilha da Trindade Rudolf Barth: a pioneering scientist on the island of Trindade

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    Ortrud Monika Barth

    2012-09-01

    Full Text Available Em consequência da instituição do Ano Geofísico Internacional, em 1957, a Marinha do Brasil organizou uma expedição à ilha da Trindade, da qual participou Rudolf Barth, pesquisador do Instituto Oswaldo Cruz e do Instituto de Pesquisas da Marinha, membro da Academia Brasileira de Ciências e do Conselho Nacional de Pesquisas. Esse cientista retornou à ilha da Trindade no ano seguinte, em uma segunda viagem com a Marinha brasileira. As pesquisas feitas então resultaram em algumas publicações científicas e em dois relatórios inéditos com observações e dados concernentes a fenômenos zoológicos (terrestres, meteorológicos, climatológicos, geomorfológicos e biológicos. Os relatórios são transcritos a seguir.As a result of the institution of the International Geophysical Year in 1957, the Brazilian Navy organized an expedition to the island of Trindade, on which Rudolf Barth participated. He was a researcher at the Oswaldo Cruz Institute and the Naval Research Institute, and a member of the Brazilian Academy of Sciences and the National Research Council. The scientist returned to the island of Trindade the following year, on a second expedition with the Brazilian Navy. The research findings then resulted in some scientific publications and two unpublished reports with observations and data concerning terrestrial, zoological, meteorological, climatological, geomorphological and biological phenomena. The reports are then transcribed.

  15. CARACTERIZAÇÃO DA REDE DE DRENAGEM NA PORÇÃO LESTE DA ILHA DO MARAJÓ E IMPLICAÇÕES TECTÔNICAS

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    Lena Simone Barata Souza

    2011-11-01

    Full Text Available A análise de bacias de drenagem, combinada com dados de sensoriamento remoto e cartográficos, fornece informações significantes para o reconhecimento de lineamentos morfoestruturais e para a detecção de feições tectônicas com expressão na paisagem atual. Este procedimento foi aplicado no estudo de três bacias de drenagem do leste da Ilha do Marajó, correspondentes aos rios Arari, Camará e Paracauari. Todas estas bacias mostram evidências de anomalias morfoestruturais, incluindo-se principalmente: mudanças rápidas no padrão de drenagem dentro de uma mesma bacia, variando de treliça, retangular, multibacinal a subparalelo; canais retilíneos comumente conectados em ângulos retos; canais fortemente sinuosos que se tornam retilíneos; meandros localizados; e bacias altamente assimétricas. Anomalias de drenagem, consistindo em frequentes desvios de direção formando ângulos retos e meandros isolados e comprimidos, são também reconhecidas na paleodrenagem preservada neste setor da ilha. Com base na densidade dos lineamentos estruturais, pode-se reconhecer dois compartimentos morfoestruturais. O compartimento I é localizado na porção centro-leste da área de estudo, onde ocorre densidade de lineamento alta com duas direções preferenciais para NW-SE e NE-SW. O compartimento II, correspondente ao restante da área, apresenta densidade de lineamentos variando entre muito baixa a média e com orientação principal para NE-SW. As características morfoestruturais dos sistemas de drenagem atual e pretérita permitem sugerir área com forte controle tectônico. A deformação tectônica pode refletir reativação de falhas de direções principais NW-SE e NE-SW, melhor registradas no compartimento I.

  16. Construindo vidas na diáspora. Os africanos da cidade do Desterro, Ilha de Santa Catarina (Século XIX

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    Claudia Mortari Malavota

    2013-06-01

    Full Text Available Este artigo tem como objetivo evidenciar e analisar os vínculos parentais estabelecidos por escravos e libertos de procedência africana, sujeitos de diferentes categorias sociais e origens étnicas, no contexto de uma cidade portuária ao Sul do Brasil: Nossa Senhora do Desterro, localizada na Ilha de Santa Catarina. O ponto de partida do artigo é a trajetória de vida, construída a partir de alguns indícios, do africano forro Francisco de Quadros, da africana Catharina, de nação Benguela, e de Francisco de Siqueira, os quais, no contexto do século XIX, estabeleceram relações de solidariedade e vínculos familiares. A partir de suas trajetórias, nossa intenção é descortinar outras inúmeras experiências de vida das populações de origem africana na cidade. Partimos do princípio de que os estabelecimentos de vínculos parentais constituem, num contexto escravista, uma maneira de criar esperanças, de possibilitar a sobrevivência e de reinventar as identidades. Os africanos, ao criar seus vínculos familiares, conferiram sentido a suas vidas e marcaram de forma significativa o espaço social em que viviam.

  17. Viabilidade ambiental para a criação de unidades de conservação na Ilha da Coroa, Mossoró – RN / Environment feasibility for creating conservation units in Ilha da Coroa, Mossoró, Brazil

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    Marisa Carvalho Varela

    2009-08-01

    Full Text Available This paper aims at studying an area inserted in the fluvial lowland of the Apodi-Mossoró river. It isa fluvial island called Ilha da Coroa, situated in the Alagados neighborhood, in the urban area ofthe municipality of Mossoró. Many environmental parameters were evaluated in order to verify thefeasibility of creating conservation units. In order to reach this goal we researched the bibliographyand the legislation related to the topic, analyzed aerial photos, applied questionnaires with residentsand users of the area, visited public institutions for collecting data and checked the field. Eventhough most of the indicators are not favorable, the possibility of creating conservation units inIlha da Coroa should not be discarded, due to the great fragility of the fluvial ecosystems associatedto the Apodi-Mossoró river.

  18. Caracterização hidrodinâmica e do transporte de sedimentos na região fluvio-estuarina do rio Paracauari, Ilha de Marajó, Pará

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    Ferreira, Gabriela Padilha

    2013-01-01

    O Rio Paracauari é o principal rio que drena a porção nordeste da Ilha de Marajó, estado do Pará, maior ilha fluvio‐estuarina do mundo, e que integra a rede fluvial da foz da Bacia Hidrográfica do Rio Amazonas. O presente trabalho visou caracterizar o funcionamento hidrodinâmico do baixo‐estuário rio Paracauari e quantificar o aporte de sedimentos do mesmo para a baía de Marajó em função do regime sazonal das chuvas na região, com amostragens durante os períodos de maior volume...

  19. Sensoriamento remoto na identificação do uso e ocupação da terra na área do projeto "Conquista da Água", Ilha Solteira - SP, Brasil Remote sensing on the identification of the use and occupation of land of the project "Management of the Water", Ilha Solteira - State of São Paulo, Brazil

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    Hélio R. Silva

    2006-04-01

    Full Text Available A cidade de Ilha Solteira está propondo o projeto "Conquista da Água", visando ao desenvolvimento sustentável do Município, a partir do turismo, da cultura e da ciência e tecnologia. Para atingir esse objetivo, os planejadores do meio físico-ambiental necessitam de dados que possam auxiliá-los na seleção dos melhores locais onde serão instalados as avenidas, o aeroporto e os demais espaços que comporão esse projeto. A elaboração do mapa de uso e cobertura do solo da área de interesse do projeto constitui um dos temas necessários ao banco de dados a ser utilizado pelos planejadores. Neste trabalho, é apresentado o estado de degradação dessa área, o que auxiliará na definição das estratégias de conservação ambiental.The City hall of Ilha Solteira is proposing the project "Management of the Water", seeking to contribute with the sustainable development of the municipal district, through tourism, culture, science and technology. To reach it, the planners of the physical-environmental way, will need data that will aid in the selection of the best local to install the avenues, the airport and other spaces that will compose this project. The elaboration of a Map of the current use and cover of the lands in the area of interest of the project represents one of the necessary themes to compose a database to be used by the planners. In the present work, it is presented a discussion on the state of degradation of the landscape, which will help the definition of strategies of environmental conservation.

  20. Degradação Ambiental e Hospitalidade: apontamentos sobre a intensificação do turismo na Vila do Abraão Ilha Grande - RJ, Brasil

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    Carolina Dutra Araújo

    2013-02-01

    Full Text Available O presente artigo tem como objetivo avaliar a percepção do residente da Ilha Grande, em relação ao impacto ambiental causado pelo turismo e contrapor à visão do turista, a fim de demonstrar se há algum reflexo do impacto ambiental na hospitalidade local. Sobre esse aspecto, colocam-se duas importantes questões: se o residente credita o impacto ao visitante, como ele se comporta em relação ao turismo; se esse comportamento é negativo, como o visitaJ;lte percebe a hospitalidade local. Ao longo das discussões em torno dessas problemáticas, surgiu uma nova indagação: se o impacto é visível, como ele repercute na motivação do turista ao viajar para a Ilha Grande e qual a imagem que ele possui do lugar, em contraposição à imagem do residente. The objective of this paper is to evaluate the perception of Ilha Grande's residents related to the environmental impact caused by tourism and to contrast to tourist's point of view, as to demonstrate if there is any consequence of the environmental impact on local hospitality. On this aspect two important questions are made: if residents think the impact is caused by visitors, how do they behave due to tourism; if this behavior is negative, how do visitors interpret local hospitality. During the discussions about this problem, a new question appeared: if the impact is visible, how does it reflects on tourist's motivation to travel to Ilha Grande and what is the image that they have about the place, contrasting to resident's image.

  1. Impacto da geometria do cânion urbano na intensidade de ilha de calor noturna: análise através de um modelo simplificado adaptado a um SIG

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    Camila Mayumi Nakata-Osaki

    Full Text Available Resumo A geometria urbana é um dos fatores de maior influência na intensidade da ilha de calor urbana. Seu estudo requer a caracterização de cânions urbanos, geralmente medidos pela relação entre a altura dos edifícios e a largura da rua (H/W, conceito aplicado no modelo numérico de Oke em 1981. O objetivo deste artigo é verificar o impacto da geometria do cânion urbano na intensidade de ilhas de calor noturna. Para isso, foram realizados levantamento de dados climáticos e de geometria urbana em duas cidades brasileiras. Os valores de intensidade de ilha de calor foram confrontados com os simulados pelo modelo original de Oke (1981, o qual foi calibrado e adaptado à plataforma SIG, de forma a possibilitar a incorporação de outro parâmetro de geometria, além da relação H/W: o comprimento de rugosidade. Esse processo gerou uma nova ferramenta de cálculo, que é denominda THIS (Tool for Heat Island Simulation. Aplicou-se o novo modelo para simular alguns cenários urbanos hipotéticos, que representam vários tipos de cânions urbanos. Os resultados demonstraram que cânions urbanos de maior rugosidade amenizam as intensidades de ilha de calor noturna em relação a um cânion de mesmo valor de relação H/W e menor rugosidade.

  2. JORNAL O NACIONAL E BAIRRO LEONARDO ILHA: AS RELAÇÕES TRANSLINGÜÍSTICAS NA PRODUÇÃO DE SENTIDO

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    Roberto José Ramos

    2006-08-01

    Full Text Available O presente ensaio é um estudo semiológico que tem como objeto de investigação a produção de sentido verbal e não-verbal de uma notícia, ilustrada com foto, publicada no Jornal O Nacional em Passo Fundo/RS sobre a comunidade de moradores do bairro Leonardo Ilha, localizado na zona urbana da mesma cidade. O teórico norteador da pesquisa é Roland Barthes, por intermédio de cinco categorias: Discurso (Pirâmides Normal, Invertida e Mista e Estereótipo; Fotografia (Studium e Punctum; Mito; Poder e Socioleto (Encrático e Acrático. Através das reflexões deste autor, o trabalho busca estabelecer relações entre o lingüístico e o translingüístico no acontecimento registrado. O método utilizado é a Dialética Histórico- Estrutural (DHE, que compatibiliza os princípios de movimento próprio da Dialética, e a invariância, própria do Estruturalismo, explicando a idéia de realidade, através de elementos formais e históricos, pertinentes à escolha do objeto e dos objetivos do ensaio. Palavras-chave: Jornalismo. Produção de sentido. Roland Barthes.

  3. Fauna de formigas como ferramenta para monitoramento de área de mineração reabilitada na Ilha da Madeira, Itaguaí, RJ.

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    Marcos Paulo dos Santos Pereira

    2010-08-01

    Full Text Available A riqueza e diversidade de espécies de formigas podem ser maiores em ambientes de complexidade mais elevada devido a uma maior disponibilidade de nichos presentes. Em razão da conexão entre os organismos e as características dos habitats, as formigas têm sido utilizadas como ferramentas no monitoramento ambiental de áreas perturbadas. Neste estudo, foi investigada a estrutura da comunidade de formigas em parcelas utilizadas para reabilitação de uma área de mineração na Ilha da Madeira, Itaguaí, RJ. Encontrou-se uma fauna diferenciada de formigas e maior riqueza de espécies em parcelas nas quais foi utilizado um maior número de espécies arbóreas nativas do que em parcelas com poucas espécies arbóreas ou sem nenhuma intervenção conservacionista.

  4. OFICINA DE INTERPRETAÇÃO AMBIENTAL COM ALUNOS DO ENSINO FUNDAMENTAL NA “TRILHA DO JATOBÁ” EM ILHA SOLTEIRA, SP. ENVIRONMENTAL INTERPRETATION WORKSHOP WITH ELEMENTARY SCHOOL STUDENTS IN THE “JATOBÁ TRAIL”, ILHA SOLTEIRA, SP

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    Cinthia Montibeller Santos

    2012-11-01

    Full Text Available As trilhas para a interpretação de áreas naturais têm sido recomendadas em programas de Educação Ambiental por oferecerem contato direto com o ambiente natural, direcionando o aprendizado e a sensibilização. Apesar da premissa eficácia e da frequência com que têm sido implantadas, existem poucos estudos que indiquem sua validade educacional. O presente trabalho utilizou a Trilha do Jatobá, localizada no Centro de Conservação da Fauna Silvestre (CCFS-CESP - Ilha Solteira - SP, Brasil, como instrumento de aprendizagem de conteúdos de ciências por meio de interpretação da paisagem e de seus componentes. Os objetivos foram: 1 analisar a validade da execução de atividades práticas nesse ambiente natural e 2 verificar a eficiência desta atividade quando precedida de uma aula teórica. A oficina, dividida em aula teórica e prática, foi oferecida para 30 alunos do 7º ano do ensino fundamental de uma escola particular do município de Ilha Solteira (SP, divididos em dois grupos de 15 pessoas. O primeiro assistiu a uma aula teórica enquanto o outro fez o percurso pela trilha e, depois de concluídas tais atividades, os grupos foram invertidos. Foram aplicados três questionários: 1º. Diagnosticar o conhecimento prévio; 2º. Após a aula teórica e 3º Após a aula prática (trilha para verificação do desempenho dos alunos. De acordo com os resultados a oficina foi satisfatória para todos os alunos, ressaltando que aqueles que assistiram à aula teórica antes do percurso pela trilha tiveram melhor aproveitamento.Trails for interpretation of natural areas have been recommended in Environmental Education programs because they offer direct contact with the natural environment, addressing learning and consciousness. Despite the assumed effectiveness and the frequency with which they have been deployed, there are few studies that indicate their educational validity. The present study used the Trilha do Jatobá (Jatobá Trail

  5. Dinâmica Glacial e Características Sedimentares Resultantes na Zona Proglacial da Geleira Ecology-Baía do Almirantado, Ilha Rei George Antártica.

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    Kátia Kellem Rosa

    2006-12-01

    Full Text Available A geleira Ecology, localizada na ilha Rei George, ilhas Shetlands do Sul, está sofrendo um processo de rápida retração ao longo das últimas décadas, sendo que no período de 1956 a 1992/95 a geleira retrocedeu 0,37 Km2. Isto gerou um ambiente de deglaciação, com a exposição de várias geoformas na zona proglacial. Este trabalho objetiva estudar o ambiente de deglaciação da zona proglacial da geleira Ecology e seus respectivos depósitos na zona proglacial, além de inferir o regime termo-basal desta geleira. O produto resultante deste trabalho é a criação de um modelo geomorfológico para a zona proglacial da geleira, o qual pode ser usado para comparar com zonas proglaciais de outras geleiras.

  6. FORÓFITOS PREFERENCIAIS DE ORQUÍDEAS EPÍFITAS NA APA ILHA DO COMBU, BELÉM, PARÁ, BRASIL

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    Tonny David Santiago Medeiros

    2014-10-01

    Full Text Available Foi avaliada a correlação da abundância de orquídeas epífitas com o tipo de casca dos forófitos e a influência do DAP sobre a abundância e a riqueza na Área de Proteção Ambiental (APA Ilha do Combu, Belém, Pará, Brasil. O estudo foi realizado em 2,85 ha, a partir da demarcação de 114 transectos de 5 m x 50 m, onde foram mensurados todos os forófitos com DAP ≥ 20 cm e anotadas as características da casca, e identificadas as orquídeas epífitas. A correlação entre o tipo de casca dos forófitos e a abundância de orquídeas foi verificada através do número de epífitas por forófitos na espécie arbórea x, e também da razão (n.° epífitas/n.° forófitos para forófitos rugosos e não-rugosos. Os diâmetros foram categorizados em classes para verificar a influência do DAP sobre a abundância e a riqueza. Trinta e cinco espécies de forófitos (73% apresentaram casca rugosa, com destaque para Hevea brasiliensis e Carapa guianensis que foram os mais amostrados (58 e 42 e mais expressivos em número de orquídeas epífitas; a razão de epífitas por forófitos foi maior em troncos com casca não-rugosa. As classes de diâmetro 2 (31,8┤ 63,7 e 3 (63,7┤ 95,5 registraram as maiores abundâncias de orquídeas epífitas. A abundância de orquídeas epífitas é influenciada pela rugosidade da casca e correlacionada aos maiores diâmetros dos forófitos. Palavras-chave: interação, hospedeiro, orquidaceae, floresta de várzea, Amazônia. DOI: http://dx.doi.org/10.18561/2179-5746/biotaamazonia.v4n3p1-4

  7. Unidades habitacionais hoteleiras na Ilha de Santa Catarina: um estudo sobre acessibilidade espacial Habitational units in hotels of the Island of Santa Catarina: a study about Spatial accessibility

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    Vera Helena Moro Bins Ely

    2009-01-01

    Full Text Available Este artigo trata da avaliação das condições de acessibilidade espacial em unidades habitacionais (UHs, ditas acessíveis, de hotéis residenciais na ilha de Santa Catarina. Para tanto, visitas exploratórias permitiram o levantamento arquitetônico das UHs escolhidas e a análise de sua adequação aos parâmetros técnicos estabelecidos pela NBR 9050/2004. Entrevistas com diferentes usuários complementam o estudo na medida em que apontam as reais dificuldades enfrentadas por eles, devido às inadequadas soluções de acessibilidade presentes nos ambientes da rede hoteleira. Os métodos utilizados permitiram classificar os problemas de acessibilidade espacial segundo quatro componentes - orientação espacial, deslocamento, uso e comunicação - e relacioná-los às deficiências e habilidades dos diversos usuários. Encontrou-se maior prevalência de problemas ligados ao uso de mobiliários e equipamentos, os quais afetam a realização de atividades com conforto e independência. Verifica-se, também, que a NBR 9050/2004 ainda direciona seus parâmetros técnicos para atender principalmente deficientes físico-motores.This paper deals with the evaluation of the conditions of spatial accessibility in habitation units (UHs considered accessible in residential hotels on the Island of Santa Catarina. For this purpose, exploratory visits allowed an architectonic survey of the chosen UHs and an analysis of their adjustment to the technical parameters established by NBR 9050/2004. Interviews with different users completed the study which highlighted the real difficulties faced by them due to inadequate solutions of accessibility present in the hotel network environments. The methods facilitated a classification of the problems of spatial accessibility according to four components - spatial orientation, displacement, use and communication - and related them to the abilities and disabilities of the various users. A higher prevalence of problems

  8. Ilhas Crioulas: o significado plural da mestiçagem cultural na África Atlântica

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    Roquinaldo Ferreira

    2006-12-01

    Full Text Available O objetivo deste artigo é analisar a interação cultural na África Atlântica, principalmente em Angola. O caso angolano é comparado com outras regiões da África, tais como a Senegâmbia, Costa do Ouro, Benin e Baía de Biafra. Tanto quanto Angola, estas regiões foram afetadas pelo tráfico de escravos, integrando de forma diferenciada as redes de comércio Atlânticas. Em Angola, a mestiçagem cultural alcançou níveis mais intensos do que no restante da África Atlântica, em virtude da maior intensidade e duração do tráfico, assim como do processo de "interiorização" de interesses luso-africanos na região do hinterland de Luanda, na primeira metade do século XIX.

  9. Distribuição de Aedes aegypti e do dengue no Estado do Maranhão, Brasil

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    Rebêlo José Manuel Macário

    1999-01-01

    Full Text Available O dengue e o Aedes aegypti encontram-se disseminados em municípios de todas as regiões do Maranhão. No ano de 1995, foram trabalhados 87 dos 136 municípios em que se divide geograficamente o Estado, 176 localidades e 480.687 imóveis. Foram considerados positivos para Ae. aegypti trinta municípios (34,4%, 118 localidades (67,0% e 10.357 imóveis (2,1%. Dos municípios positivos, três pertencem à Ilha de São Luís, sete à Amazônia Maranhense, 12 à zona dos cerrados meridionais, cinco à zona mista de matas-cerrados-cocais. Nas zonas que seguem - campos aluviais, matas-cocais e dunas-restinga -, Ae. aegypti foi encontrado em apenas um município. Os índices de positividade predial foram mais elevados na Amazônia Maranhense (3,5% e na Ilha de São Luís (2,5%, por constituírem as rotas de maior fluxo migratório da população e de escoamento de produtos entre o Maranhão e os estados vizinhos e também por serem áreas onde estão localizados os grandes centros urbanos e econômicos do Estado. Os índices de infestação predial por Ae. aegypti e de casos de dengue notificados foram maiores nos meses úmidos, mostrando a importância das chuvas na formação de criadouros do vetor e na distribuição de Aedes e do dengue.

  10. Leishmaniose tegumentar americana na Ilha Grande, Rio de Janeiro: VI. observações sobre a freqüência horária e variação mensal dos transmissores

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    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Para estudo da freqüência horária dos principais vetores da Leishmaniose Tegumentar Americana (LTA, na Ilha Grande, durante os anos de 1976 a 1977, foram realizadas 4 capturas de 24 horas com isca animal (cão, ao ar livre, e 5 capturas de 24 horas em um único domicilio da área. Observou-se que a espécie Lutzomyia intermedia ocorreu durante todo o período noturno, com densidade constante no domicílio humano, enquanto a espécie Lutzomyia migonei apresentou maiores densidades no período da madrugada, nas capturas com isca animal (cão. Em 156 horas de estudo sobre variação mensal, durante o período de março de 1976 a fevereiro de 1977, a espécie L. intermedia ocorreu durante todo o ano, sendo abril, maio, outubro e dezembro os meses de maior densidade; a espécie L. migonei apresentou-se com baixa densidade, chegando a desaparecer no mês de setembro. As elevadas densidades das espécies L. intermedia e L. migonei indicam-nas como as prováveis vetoras de LTA na Ilha Grande.For the study of time frequency of the principal vectors of the Mucocutaneous Leishmaniasis in the Ilha Grande (Great Island, from 1976 to 1977 we made four catches in 24 hours with animal bait (dog in the outdoors and five catches in just one house of the area. It was found that the species Lutzomyia intermedia occurred during the whole night a steady density in human dwellings, while the species Lutzomyia migonei presented greater density at dawn, in catches with animal bait (dog. in a 156 hours' study on monthly variation, from March 1976 to February 1977, a specie Lutzomyia intermedia appeared during the whole year, the months of April, May, October and December being those of greater density while the specie Lutzomyia migonei had a low density even disappearing in September. The high densities of the species Lutzomyia intermedia and Lutzomyia migonei indica te them as the probable vectors of the mucocutaneous leishmaniasis in Ilha Grande.

  11. Leishmaniose tegumentar americana na Ilha Grande, Rio de Janeiro: VI. observações sobre a freqüência horária e variação mensal dos transmissores

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    Nelson A. de Araújo Filho

    1981-12-01

    Full Text Available Para estudo da freqüência horária dos principais vetores da Leishmaniose Tegumentar Americana (LTA, na Ilha Grande, durante os anos de 1976 a 1977, foram realizadas 4 capturas de 24 horas com isca animal (cão, ao ar livre, e 5 capturas de 24 horas em um único domicilio da área. Observou-se que a espécie Lutzomyia intermedia ocorreu durante todo o período noturno, com densidade constante no domicílio humano, enquanto a espécie Lutzomyia migonei apresentou maiores densidades no período da madrugada, nas capturas com isca animal (cão. Em 156 horas de estudo sobre variação mensal, durante o período de março de 1976 a fevereiro de 1977, a espécie L. intermedia ocorreu durante todo o ano, sendo abril, maio, outubro e dezembro os meses de maior densidade; a espécie L. migonei apresentou-se com baixa densidade, chegando a desaparecer no mês de setembro. As elevadas densidades das espécies L. intermedia e L. migonei indicam-nas como as prováveis vetoras de LTA na Ilha Grande.

  12. Avaliação da fluoretação da água do sistema de abastecimento público na Ilha de São Luís, Maranhão, Brasil

    OpenAIRE

    Carmo,Cadidja Dayane Sousa do; Alves,Cláudia Maria Coelho; Cavalcante,Paulo Roberto; Ribeiro,Cecília Cláudia Costa

    2010-01-01

    O objetivo deste estudo foi avaliar os níveis de flúor na água de abastecimento público da Ilha de São Luís (MA). Aplicou-se uma amostragem estratificada, considerando-se as estações de tratamento de água (06) e os bairros abastecidos (46). A análise dos teores de flúor na água foi feita através de um eletrodo específico. A concentração nas amostras variou de 0,05 a 0,84 ppm de flúor. Apenas uma estação de tratamento (Italuís) apresentou-se dentro do limite aceitável de fluoretação. A fluoret...

  13. Pelas trilhas da ILha de Santa Catarina

    OpenAIRE

    Silveira, Flavio Leonel Abreu da

    1996-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciencias Humanas O estudo sobre o ecoturismo na Ilha de Santa Catarina vem demonstrar o surgimento de novas modalidades turísticas entre os grupos urbanos. A pesquisa aponta para a intersecção entre lazer, turismo, ecologia e esporte, demonstrando a importân-cia das práticas ecoturísticas na atualidade. A partir de tal perspectiva, as questões de gênero surgem com significativa relevância. Estamos frente...

  14. Criadouros de Aedes (Stegomyia aegypti (Linnaeus, 1762 em bromélias nativas na Cidade de Vitória, ES Aedes (Stegomyia aegypti (Linnaeus, 1762 breeding sites in native bromeliads in Vitória City, ES

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    José Benedito Malta Varejão

    2005-05-01

    Full Text Available Alguns insetos transmissores de doenças procriam exclusivamente nas proximidades das residências. O Aedes aegypti, responsável por epidemias de dengue em cidades brasileiras, representa sério risco também para a febre amarela. Com o insucesso da campanha de erradicação do inseto, justifica-se a busca de criadouros fora do alcance das medidas de controle atualmente adotadas. Na Cidade de Vitória, ES, investigou-se a ocorrência de criadouros de Aedes aegypti na água coletada em bromélias nativas, sobre as rochas. Paralelamente, avaliou-se a infestação predial nas áreas urbanas contíguas. Em quatro das cinco áreas investigadas foram encontradas larvas de culicídeos nas bromélias, sendo que em duas foi identificado Aedes aegypti. A presença dos criadouros em bromélias não guardou relação com a infestação predial nas áreas próximas. Torna-se necessário definir se os criadouros em bromélias constituem focos primários do Aedes aegypti, ou se representam uma conseqüência da elevada infestação urbana.Some insects that are vectors of human diseases have accompanied man in his migrations throughout the world and breed exclusively in the proximity of human dwellings. The mosquito Aedes aegypti has been responsible for epidemics of dengue in Brazil and its presence also constitutes a serious risk for future outbreaks of urban yellow fever. The failure of campaigns to eradicate this species justifies the search for alternative breeding sites, which may be beyond the reach of present control measures. In this study the occurrence of Aedes aegypti breeding sites in native bromeliads on rocky slopes was investigated in five areas of Vitória, capital of the Brazilian State of Espírito Santo, ES. Water contained in the bromeliads was collected with the aid of a suction apparatus to search for culicid larvae. The degree of infestation of buildings in adjacent urban areas was evaluated simultaneously. Culicid larvae were found in

  15. Percepção ambiental e narrativas visuais da transformação da paisagem e suas relações com o turismo na Ilha Comprida (SP)

    OpenAIRE

    Carmacio Junior, Izaias; Centro Universitário Fundação Santo André, Santo André, SP; Rodrigues, Diogo Fernando; Centro Universitário Fundação Santo André, Santo André, SP; Vaz de Figueiredo, Luiz Afonso; Centro Universitário Fundação Santo André, Santo André, SP

    2015-01-01

    O município de Ilha Comprida está localizado na região do baixo Vale do Ribeira e Litoral Sul do estado de São Paulo, sendo emancipado politicamente em 1992. Possui vegetação nativa de Mata Atlântica, associada com manguezais, restingas e dunas, além de extensa faixa litorânea, incluído em 1987 em uma Área de Proteção Ambiental (APA). A localidade está sujeita às atividades de turismo e segunda residência, sofrendo problemas socioambientais com o processo de urbanização e construção da ponte ...

  16. Amostragem por larva-única na vigilância de Aedes aegypti Single-larva sampling for Aedes aegypti surveillance

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    José Eduardo Bracco

    1995-04-01

    Full Text Available Com a finalidade de testar a metodologia de amostragem por larva-única na vigilância entomológica do Aedes aegypti, foram pesquisados domicílios do Município de Araraquara, SP (Brasil. Nos criadouros que continham larvas de Aedes uma delas foi coletada. Como controle, após a coleta da larva-única, todas as larvas foram coletadas para identificação posterior. Esse processo foi repetido no laboratório. Dos 447 domicílios visitados, apenas 12 foram considerados positivos e 20 criadouros foram identificados; destes, 13 continham larvas de Aedes; 5, larvas de Aedes e Culex e 2, larvas de Culex. Os resultados mostram o reconhecimento correto, no campo, de todos os criadouros, evidenciando que o método poderia ser utilizado na vigilância entomológica de municípios sem infestação domiciliar ou infestados apenas com uma única espécie de Aedes.Buildings in Araraquara city, Southeastern Brazil, were searched during a year for the presence of Aedes larvae using single larva sampling in order to check the single-larva methodology. In those breeding places in wich Aedes larvae were found, one of them was collected. As a control, after the single larva had been collected, all the larvae from the breeding place were collected for later identification. This process was repeated in the laboratory. Of the 447 domiciles searched, 12 were considered positive and 20 breeding places were found. Of the breeding places, 13 contained Aedes larvae, 5 both Aedes and Culex larvae and 2 Culex larvae only. The results show that all the breeding places in the field were properly recognited showing the method may be used for Aedes surveillance in cities infested with one species only or without any domiciliary infestation.

  17. Strong alkalinization in the anterior midgut of larval yellow fever mosquitoes (Aedes aegypti): involvement of luminal Na+/K+-ATPase.

    Science.gov (United States)

    Onken, Horst; Patel, Malay; Javoroncov, Margarita; Izeirovski, Sejmir; Moffett, Stacia B; Moffett, David F

    2009-03-01

    Recently, Na(+)/K(+)-ATPase has been detected in the luminal membrane of the anterior midgut of larval yellow fever mosquitoes (Aedes aegypti) with immunohistochemical techniques. In this study, the possible involvement of this ATPase in strong alkalinization was investigated on the level of whole larvae, isolated and perfused midgut preparations and on the molecular level of the Na(+)/K(+)-ATPase protein. Ouabain (5 mM) did not inhibit the capability of intact larval mosquitoes to alkalinize their anterior midgut. Also in isolated and perfused midgut preparations the perfusion of the lumen with ouabain (5 mM) did not result in a significant change of the transepithelial voltage or the capacity of luminal alkalinization. Na(+)/K(+)-ATPase activity was completely abolished when KCl was substituted with choline chloride, suggesting that the enzyme cannot act as an ATP-driven Na(+)/H(+)-exchanger. Altogether the results of the present investigation indicate that apical Na(+)/K(+)-ATPase is not of direct importance for strong luminal alkalinization in the anterior midgut of larval yellow fever mosquitoes.

  18. Análise espaço temporal da evapotranspiração na área de preservação ambiental da Ilha de Santa Rita, Alagoas, Brasil

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    Admilson Penha Pacheco

    2014-06-01

    Full Text Available A evapotranspiração (ET é uma das principais componentes do ciclo hidrológico. Nesse contexto, este trabalho teve como objetivo analisar o comportamento espaço-temporal do Balanço de Energia e da Evapotranspiração Real Diária na Área de Preservação Ambiental da Ilha de Santa Rita, Alagoas, Brasil, tomando como subsídio as possíveis alterações no uso e cobertura do solo. A metodologia utilizada envolve o uso do algoritmo SEBAL, imagens do satélite Landsat 5 - TM e dados complementares de estação meteorológica localizada na área de estudo. A estimativa diária de ET24h real variou de 0 a 1 mm dia-1 no solo exposto e na cidade; a 6 mm dia-1, em corpos d'água e mangue, com um valor médio de 3,78 mm dia-1, 3,45 mm dia-1 e 4,75 mm dia-1, nos dias 03/09/2003, 26/08/2006 e 17/03/2011, respectivamente, em toda a área estudada. Os resultados por meio do algoritmo SEBAL (SURFACE ENERGY BALANCE ALGORITHMS FOR LAND podem ser utilizados em condições climáticas semelhantes às da Área de Preservação Ilha de Santa Rita/Alagoas. As distribuições de cada componente do balanço de energia: saldo de radiação, fluxo de calor no solo, sensível e latente, apresentaram influência significativa nos diferentes tipos de uso e cobertura do solo. Além disso, constataram-se diferenças evidentes entre os fluxos em zona rural e área urbana. A abordagem utilizada é muito adequada para uma exploração real de dados de satélite, podendo ser útil para estimar um número de parâmetros no contínuo solo-planta-atmosfera.

  19. Dirofilariose canina na Ilha de São Luís, Nordeste do Brasil: uma zoonose potencial Canine heartworm on São Luís Island, Northeastern Brazil: a potential zoonosis

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    Silvia Maria Mendes Ahid

    1999-04-01

    Full Text Available Um inquérito sobre a prevalência da dirofilariose canina foi realizado entre 1.495 cães de 64 localidades na Ilha de São Luís, de 1991 a 1994, através da pesquisa de microfilárias. Desses, 1.358 cães (12,8% positivos não tinham história pregressa conhecida, sendo 1.265 errantes (10,3% microfilarêmicos e 93 domiciliados (37,8%. A dirofilariose foi detectada em 46 das localidade, porém a prevalência da infecção aumenta consideravelmente quando se trata de cães da orla marítima, de onde procederam 47% dos animais positivos. Em alguns bairros costeiros, a prevalência é elevada, como em Olho d'Água e Calhau (46% e 43% positivos, respectivamente. A pesquisa de microfilárias em 137 cães estritamente domiciliados (DO e com história pregressa conhecida (43% microfilarêmicos permitiu a comprovação da existência de transmissão na Ilha. Este estudo é o primeiro dessa natureza e abrangência realizado no Nordeste do País. Sabendo-se que a dirofilariose é uma zoonose, e diante da elevada prevalência de cães microfilarêmicos, particularmente os domiciliados da orla marítima da Ilha, chama-se a atenção para que se considere tal parasitose como possível causa de lesões pulmonares solitárias na população humana local e naquelas assentadas em áreas com clima e paisagem semelhantes no Nordeste.A survey on the prevalence of canine heartworm was conducted in 1,495 dogs from Maranhão Island, State of Maranhão, Northeastern Brazil, from 1991 to 1994, by testing for microfilariae in blood. Of the total, 1,358 (12.8% of which were infected were dogs with no known history; they included 1,265 homeless animals (10.3% with microfilariae and 93 kept by owners at the time the survey was conducted (37.8% of which were infected. Prevalence is high among dogs captured and/or living along the seashore. Examples of such high coastal prevalence rates were found in Olho d'Água and Calhau (46% and 43%, respectively. The search for

  20. Estudo do Fenômeno da Ilha de Calor na Cidade de Manaus/AM: Um Estudo a Partir de Dados de Sensoriamento Remoto, Modelagem e Estações Meteorológicas

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    Polari Batista Corrêa

    2016-06-01

    Full Text Available Resumo O fenômeno da ilha de calor (IC na cidade de Manaus foi estudado usando dados de modelagem do clima urbano com o modelo Weather Research and Forecasting (WRF e dados de estações meteorológicas. Foram usados os dados de temperatura da superfície (TS obtidos pelo sensor MODIS do satélite ambiental Aqua, produto MYD11A2 na resolução horizontal de 1 km por um 1 km para o período de 2002 a 2012 na cidade de Manaus para avaliar o processo de urbanização. Foi realizado um estudo de caso com as simulações de TS e de temperatura do ar (TA nos meses de agosto e setembro de 2009 que foram comparadas, respectivamente, com os dados de satélite e de estações meteorológicas. Identificou-se a distribuição espacial da TS na cidade, e as diferenças de TS entre a área urbana e a de floresta e entre a área urbana e de rios foram quantificadas. As diferenças de TS ao longo das faixas de longitude de 60,03° O a 59,97° O e de latitude de 3,09° S a −3,03° S foram analisadas com os dados do modelo WRF e do sensor MODIS. A intensidade da IC estimada com os dados de TA do modelo e de estações meteorológicas foram, em média, respectivamente de 1,87 °C e de 1,80 °C, consistentes com resultados anteriores. Portanto, a TA simulada pode ser usada para fins de estimar a intensidade da IC em Manaus.

  1. Sistema de espaços livres e forma urbana na Ilha de Vitória, Espírito Santo, Brasil

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    Eneida Maria Souza Mendonça

    2014-06-01

    Full Text Available O debate apresentado neste artigo trata das relações entre o sistema de espaços livres e a forma urbana de Vitória, capital do Estado do Espírito Santo, no Sudeste brasileiro, no território específico referente à ilha. A decisão quanto à área de estudo tem como fator fundamental o fato desta ter abrigado a origem da ocupação do lugar, em meados do século XVI, por colonizadores portugueses, e, portanto, conter, desde então, sucessivas e diversificadas formas de ocupação. A metodologia adotada compreende, principalmente, o levantamento dos espaços livres públicos e privados por imagens de satélite, e, quando necessário, visitas de campo, e o estudo relacionado à evolução urbana. A pesquisa possibilitou a identificação de morfologias distintas, caracterizadas em função do processo histórico de ocupação urbana da área e sua relação com o sítio físico. Cabe ainda observar que o território insular, constituído originalmente por morros e estreita faixa de terra entre estes e o mar, identificado como porto seguro pelos colonizadores portugueses, só evoluiu para o atual centro metropolitano em função dos sucessivos aterros realizados ao longo do tempo.

  2. Diet of the freshwater stingray Potamotrygon motoro (Chondrichthyes: Potamotrygonidae on Marajó Island (Pará, Brazil Dieta da raia de água doce Potamotrygon motoro (Chondrichthyes: Potamotrygonidae na Ilha de Marajó (Pará, Brasil

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    MP. Almeida

    2010-02-01

    Full Text Available The stomach contents of 137 examples of Potamotrygon motoro caught in 3 locations (Muaná, Afuá and Lake Arari on Marajó Island were analysed. The values of the Index of Relative Importance (IRI and its respective percentage (%IRI were calculated. The level of repletion 1 (¼ full was the most representative for both sexes, as well as for immature and mature specimens. Most of the food items found were well-digested. The food items identification indicated the presence of 15 orders, including insects, mollusks, crustaceans, annelids and fish. Differences in diet were observed among the locations studied when comparing %IRI, crustaceans being the most preferred in Afuá, fish in Lake Arari and mollusks in Muaná.O conteúdo estomacal de 137 exemplares de Potamotrygon motoro provenientes de 3 localidades (Muaná, Afuá e Lago Arari na ilha de Marajó foi analisado. Os valores do Índice Relativo de Importância (IRI e respectiva porcentagem (%IRI foram calculados. O nível de repleção 1 (¼ cheio foi o mais representativo para ambos os sexos, assim como para exemplares imaturos e maduros. A maioria dos itens alimentares analisados encontrava-se bastante digerido. A identificação dos itens alimentares indicou a presença de 15 ordens, incluindo insetos, moluscos, crustáceos, anelídeos e peixes. Diferenças na dieta entre os locais amostrados foram observadas ao se comparar as %IRI, sendo crustáceos o item preferencial em Afuá, peixes no Lago Arari e moluscos em Muaná.

  3. Fungos conidiais em Euterpe oleracea Mart. (açaizeiro na Ilha do Combu, Pará-Brasil Conidial Fungi on Euterpe oleracea Mart. on Combu Island, Pará, Brazil

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    Carla Corrêa de Castro

    2012-12-01

    Full Text Available O trabalho teve como objetivo investigar a ocorrência de fungos conidiais (hyphomycetes associados à decomposição de material vegetal morto de Euterpe oleracea Mart. (açaizeiro na Área de Proteção Ambiental da ilha do Combu, município de Belém, Pará. Entre agosto de 2008 e abril de 2009, foram coletadas partes em decomposição de palmeiras de E. oleracea e mantidas em câmara-úmida durante sete dias. As estruturas reprodutivas dos fungos foram retiradas e montadas em lâminas semipermanentes para o estudo morfológico em microscopia óptica. O presente trabalho contém descrições, comentários, distribuição geográfica e ilustrações dos novos registros para o Brasil. Como resultados deste estudo foram identificados 45 táxons de hifomicetos. Todos são novos registros para a área de estudo, destes 11 representam novos registro para o Brasil e 12 para a Amazônia brasileira.The objective of this study was look for conidial fungi (hyphomycetes associated with decomposing, dead vegetative material of Euterpe oleracea Mart. collected on Combu Island (Belém, Pará, an environmentally protected area. From August 2008 to April 2009, decomposing parts of E. oleracea were collected and kept in a moist chamber for seven days. Somatic and reproductive fungal structures were extracted from the substrate and mounted on semi-permanent slides for morphological study using optical microscopy. This paper includes descriptions, comments, geographical distributions and illustrations of new records for Brazil. All these records are new for the study area, 11 are new records for Brazil, and 12 are new for the Brazilian Amazon.

  4. GEOMORFOLOGIA E SEDIMENTOLOGIA GLACIAL APLICADAS NA RECONSTRUÇÃO DA EVOLUÇÃO DO AMBIENTE DE DEGLACIAÇÃO DA GELEIRA WANDA, ILHA REI GEORGE, ANTÁRTICA

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    Kátia Kellem Rosa

    2010-09-01

    Full Text Available Este artigo apresenta resultados das atividades de campo realizadas durante o OPERANTAR XXVI, no verão de 2007/2008, ao longo da área de deglaciação da geleira Wanda, ilha Rei George, Antártica. A geleira tem apresentado retração glacial nas últimas décadas, resultando num ambiente sedimentar, com a exposição de várias geoformas e depósitos. Esses registros geomorfológicos são úteis para a reconstrução da evolução deste ambiente de deglaciação. Os dados foram obtidos através da análise em laboratório dos sedimentos coletados em campo e posterior fotointerpretação de fotos aéreas (2003 na escala de 1:50.000, fornecidas pelo SHOA/SAF (Servicio Hidrográfico y Oceanográfico de la Armada de Chile/ Servicio Aéreo Fotogramétrico, juntamente com a análise de imagens de satélites SPOT de diferentes anos. Os resultados mostram distintas fases de deglaciação para a geleira Wanda com uma aceleração de sua retração e perda de espessura nas últimas décadas. Registros sedimentares também indicam que a geleira possui regime termal basal úmido.

  5. Análise temporal da incidência da hepatite A no município de Belém-Pa, Brasil, nos anos de 2008 e 2009 e disseminação da informação na ilha de Cotijuba

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    Lena P. S. Rodrigues; Douglas Gasparetto; João J. B. Monteiro; Nelson F.L. Soffiatti; Nelson Veiga

    2015-01-01

    A análise de dados epidemiológicos, ambientais e sócio-econômicos foi modelada para reconhecer os fatores temáticos da Hepatite A e seus mecanismos de disseminação, na ilha de Cotijuba, onde os casos ocorridos em 2008 e 2009 foram indistintamente notificados em Belém - PA. Para reconhecer a existência desses fatores foram utilizadas geotecnologias emergentes associadas a estruturas de dados relacionais e não relacionais com o método de análise temporal da relação entre os fatores e a incidênc...

  6. Um gaúcho e dezoito condores nas Ilhas Malvinas: identidade política e nação sob o autoritarismo argentino

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    Rosana Guber

    2000-10-01

    Full Text Available Argumento que o apelo ao símbolo "nação" constituía o principal veículo de construção de identidades políticas na Argentina de 1955 a 1983, sob regimes autoritários e semidemocráticos que excluíam a vontade eleitoral de amplos setores do país. Para isso, apresento a história da "Operação Condor" de 1966, na qual dezoito jovens peronistas e nacionalistas desviaram para a capital das Malvinas, sob ocupação britânica desde 1833, um vôo regular para a Patagônia. Protagonistas, simpatizantes e também antagonistas dos "condores" converteram o episódio e seus efeitos imediatos em um drama quase-turneriano no qual se encontram duas histórias: a do gaúcho Antonio Rivero e sua polêmica sublevação de 1834 contra os usurpadores ingleses, e a dos "condores". A "Operação Condor" revela assim, em última instância, a confluência de duas identidades-chave na política argentina dos anos 60 e 70: o Povo e a Juventude.I argue that the symbol of the "Nation" has been the main channel through which the formation of political identities takes place under exclusionary regimes, such as the authoritarian and restricted democracies of Argentina during the period from 1950-70. I therefore examine the "Condor Operation" of 1966, when seventeen young men and a young woman hijacked an Argentinian plane that was heading for the Malvinas (Falkland Islands, in order to assert Argentina's sovereignty over the South Atlantic archipelago which had been under British occupation since 1833. Members of the Condor group, as well as people backing and countering the Condors' action, turned the event and its effects into a quasi-Turnerian drama. It is at this point that two stories meet: that of the Gaucho Antonio Rivero and his controversial uprising against the English troops in 1834, and that of the Condors. The Condor Operation ultimately reveals the confluence of two key identities in 1960s-1970s Argentinian politics: the People and the Youth.

  7. OS IMPACTOS AMBIENTAIS E O PROCESSO DE GESTÃO INTEGRADA: EXPERIÊNCIAS NA VILA DE ABRAÃO – ILHA GRANDE, ANGRA DOS REIS (RJ / The Environmental Impacts and The Process of Integrated Management: Experiences in The Vila de Abraão – Ilha Grande, Angra dos Reis (RJ

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    Sueli Maurício Leal Filha

    2006-12-01

    Full Text Available The Ilha Grande is situated at the intersection of the parallels 23°05' and 23°14' degrees Southlatitude with the meridians 44°05' and 44°23' West longitude, in Angra dos Reis county, at the Riode Janeiro state Southern coast. It has approximately 193Km² and has two districts, Araçatiba,situated at the western portion whose main villa of residents is Provetá, and Abraão, situated atthe East portion where detaches as the main village the Vila de Abraão. The Vila de Abraão isconsidered the “capital” of the Ilha Grande, where arrive and alter on a daily basis, inhabitants,visitors, public managers, researchers and environmental defenders. The present article considersenvironmental impacts in the Vila de Abraão as important vectors in the construction of managementprocess and territorial planning, mainly after the Island’s change of function - from federal prison(Instituto Penal Cândido Mendes and environmental conservation area it becomes the headoffice of the Centro de Estudos Ambientais e Desenvolvimento Sustentável, a State University ofRio de Janeiro (UERJ unit, which has both an institutional and environmental conservation use,however framed in touristic activity growth context.

  8. No olho do furacão, na ilha da fantasia: a invenção da residência multiprofissional em saúde

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    Daniela Dallegrave

    2009-03-01

    Full Text Available Trata da Residência Multiprofissional em Saúde como uma invenção cultural constituída a partir do SUS e intenciona a inquietação e desestabilização de verdades sobre a residência multiprofissional. Utiliza o referencial teórico pós-estruturalista, com base nas ferramentas de Foucault: discurso, poder e dispositivo. Aponta que essa invenção cultural se deu com a edificação de discursos que falam sobre o que a residência é ou deveria ser. O texto procura mostrar como o tema se apresenta nos materiais produzidos e veiculados pelo Estado, pelos residentes, profissionais de saúde, programas de residência e médicos (residentes ou não. Os discursos dividem-se entre aqueles que falam a favor e contra a proposta. Neste último se incluem as produções dos homens da categoria médica, que ocupam espaços de poder. A favor, falam sujeitos sem distinção de gênero, articulam-na com outros poderosos discursos como o da integralidade e o do SUS.

  9. Inventário estruturado de formigas (Hymenoptera, Formicidae em floresta ombrófila de encosta na ilha da Marambaia, RJ Structured inventory of ants (Hymenoptera, Formicidae in atlantic slope rain-forest of Marambaia Island, RJ

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    Michel de S. Schütte

    2007-03-01

    Full Text Available As formigas são componentes funcionais importantes em florestas tropicais devido aos papéis ecológicos que exercem, à grande biomassa e à riqueza de espécies. Embora a Mata Atlântica seja um dos ecossistemas mais bem estudados no Brasil, ainda faltam informações sobre a diversidade de formigas nos fragmentos florestais do Estado do Rio de Janeiro. A riqueza e composição da assembléia de formigas em floresta ombrófila de encosta na ilha da Marambaia (RJ foi estudada através de um inventário estruturado em uma área de 0,6 ha. Armadilhas do tipo "pitfall" e coletas manuais foram empregadas na serapilheira e sobre a vegetação entre os meses de janeiro e julho de 2004. Um total de 29 gêneros e 82 espécies foi encontrado na amostragem. A abundância e a riqueza de espécies foram maiores nas amostras de março do que de julho. Já a eqüitatividade e diversidade de formigas nas amostras não foram influenciadas pela época da coleta. As amostras de formigas em galhos mortos adicionaram seis espécies à lista, acrescentando informações sobre a biologia das espécies. As amostras sobre plantas totalizaram 32 espécies de formigas, das quais 12 foram exclusivas, como as espécies de Pseudomyrmex e algumas de Crematogaster e Pachycondyla. Este estudo pretende contribuir para o desenvolvimento de prioridades conservacionistas em um dos ecossistemas mais ameaçados do mundo.Ants are an important functional component in tropical forest due to their ecological roles, biomass and species diversity. Although the Atlantic Forest is one of the best studied ecosystems in Brazil, there is a lack of information about ant diversity in forest fragments of the state of Rio de Janeiro. The composition and richness of the ant fauna from atlantic slope rain-forest in Marambaia island-RJ were assessed by the structured inventory in an area of 0.6 ha. Pitfalls traps and hand collecting were used for sampling ants in the litter and on vegetation from

  10. Focos de dirofilariose canina na Ilha do Marajó: um fator de risco para a saúde humana Focus of canine heartworm disease in Marajó Island, North of Brazil: a risk factor for human health

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    Lourdes Maria Garcez

    2006-08-01

    Full Text Available A ocorrência de dirofilariose pulmonar humana relaciona-se com a prevalência de infecção por Dirofilaria immitis na população canina. Várias espécies de mosquitos são vetores desse nematóide. Analisaram-se amostras de sangue canino coletados nas vilas Pingo d'Água e União, município de Salvaterra (Ilha do Marajó, PA, em junho, 2004 (n=34 e abril, 2005 (N=90. Os diagnósticos parasitológico e imunológico (ELISA - kit SNAP® 3DX™, Biobrasil foram comparados (chi2, alfa=0,05 no exame de 34 amostras. A prevalência na população (N=90 foi avaliada pelo ELISA. O ELISA revelou mais positivos (25/34; 73,5% que a gota espessa (23/34, 67,6% e o Knott (21/34, 61,8%, mas a diferença não foi significativa (p>0,05. A freqüência de infecção por D. immitis na faixa de 0 a 2 anos foi 58%, enquanto em cães mais velhos foi 100%. A prevalência da dirofilariose canina em Pingo d'Água e Vila União foi alta (53,5%, indicando risco de transmissão do parasito às pessoas nessa área.The occurrence of human pulmonary dirofilariasis maintains a relation with the prevalence of Dirofilaria immitis infection in the canine population. Several mosquito species are vectors of this nematode. Canine blood samples collected in Pingo d'Água and União villages, Salvaterra municipality (Marajó Island, Pará, in June, 2004 (n=34 and April, 2005 (N=90 were analyzed. Parasitological and immunological (ELISA - kit SNAP® 3DX™, Biobrasil diagnoses were compared following the examination of 34 samples. The prevalence in the population (N=90 was evaluated by means of ELISA. ELISA revealed more positive samples (25/34; 73.5% than thick smears (23/34, 67.6% or Knott (21/34, 61.8%, but the differences were not significant (p>0.05. The frequency of D. immitis infection was 58% in dogs ranging from 0-2 years old, whereas in older dogs it was 100%. The prevalence of canine dirofilariasis was high in Pingo d'Água and Vila União (53.5%, indicating the risk of

  11. OCEAN BUILDERS: INTERSEÇÕES ENTRE ECOLOGIA E ESTÉTICA NA ILHA DE SÃO VICENTE EM CABO VERDE (Dossiê: Práticas de Leitura, Livros Didáticos, Relações Raciais e as Culturas Africana e Afro-Brasileira

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    Denise Perdigão Pereira

    2017-09-01

    Full Text Available OCEAN BUILDERS: INTERACTIONS BETWEEN ECOLOGY AND AESTHETICS IN SÃO VICENTE ISLAND IN CAPE VERDEResumo:Entrevista concedida por David Leone Lima Monteiro, em janeiro de 2016, na Ilha de São Vicente, Cabo Verde. David Leone é professor de Design na Mindelo_Escola Internacional de Arte (M_EIA, localizada na referida ilha, e membro do Ocean Builders. Fundado em outubro de 2012, na Ilha de São Vicente, Ocean Builders é um movimento, ou comunidade cabo-verdiana, que se dedica à proteção ambiental, inclusive de espécies marinhas e, ainda, à sensibilização das pessoas nesse sentido. O componente estético entra como importante elemento nas ações do grupo, uma vez que seus membros e simpatizantes do movimento realizam grafites com mensagens ecológicas, bem como utilizam uma página do facebook para divulgar tanto fotografias de suas ações, como de espécies marinhas e do contexto ambiental da Ilha de São Vicente, de modo mais abrangente. Aos cinco de dezembro do ano de 2015, o grupo foi reconhecido pelo Corpo Nacional de Voluntários de Cabo Verde e pela United Nations Volunteers (UNV, devido ao “trabalho voluntário meritório em prol do desenvolvimento social e cidadania em S. Vicente”.  Palavras-chave: Ocean Builders, Ecologia, Estética. AbstractInterview grated by David Leone Lima Monteiro, in January, 2016, at São Vicente Island, Cape Verde. David Leone is Design teacher at Mindelo_Escola Internacional de Arte [Mindelo International Art School] (M_EIA, located at the referred island, and is member of Ocean Builders. Founded in October, 2012, at the Island of São Vicente, Ocean Builder is a cape Verdean movement, or community, dedicated to environmental protection, inclusive of marine species, and also to the sensitization of people in the sense. The aesthetical component figures as an important element in the group actions, since the movements’ members and supporters perform graffiti’s with ecological messages, as well

  12. Sinopse das espécies nativas e subespontâneas de Andropogoneae Dumort. (Poaceae na Ilha de Santa Catarina, Brasil A synopsis of the Andropogoneae Dumort. (Poaceae native and subspontaneous to the Island of Santa Catarina, Florianópolis, SC, Brazil

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    Bárbara Toncic Neves

    2011-12-01

    Full Text Available O presente trabalho consiste no levantamento dos táxons da tribo Andropogoneae (Panicoideae - Poaceae ocorrentes na Ilha de Santa Catarina, Florianópolis, Brasil. A partir da revisão de materiais de herbários e coletas a campo foi confirmada a ocorrência de 10 gêneros e 20 espécies, 17 nativas e duas subespontâneas: Andropogon L. (7 spp., Bothriochloa Kuntze (2, Eriochrysis P. Beauv. (1, Hyparrhenia Andersson ex E. Fourn. (1, Imperata Cirillo (1, Ischaemum L. (1, Rhytachne Desv. (1, Saccharum L. (3, Schizachyrium Nees (2 e Sorghum Moench (1. Os gêneros Bothriochloa e Rhytachne são registrados pela primeira vez para a Ilha de Santa Catarina. São apresentadas chaves de identificação para gêneros e espécies, ilustrações, informações sobre distribuição geográfica, habitat, período de floração e comentários para cada táxon.The present work is a survey of the taxa in the tribe Andropogoneae (Panicoideae - Poaceae that occur on the Island of Santa Catarina, Florianópolis, Brazil. Based on a review of herbarium specimens and fieldwork, 20 species were recorded in the following 10 genera: Andropogon L. (7 spp., Bothriochloa Kuntze (2, Eriochrysis P. Beauv. (1, Hyparrhenia Andersson ex E. Fourn. (1, Imperata Cirillo (1, Ischaemum L. (1, Rhytachne Desv. (1, Saccharum L. (3, Schizachyrium Nees (2 and Sorghum Moench (1. Seventeen of the species are native, and two species are subspontaneous. Bothriochloa and Rhytachne are recorded here, for the first time, for the Island of Santa Catarina. This work provides key to the genera and species, as well as illustrations, information about geographic distribution, habitat, phenology, and comments about each taxon presented in the study.

  13. The Macuti House in Ilha de Mocambique

    DEFF Research Database (Denmark)

    Sollien, Silje Erøy

    2014-01-01

    I afhandlingen træder en aktuel afrikansk problemstilling helt skarpt frem. Ilha de Mozambique er erklæret verdenskulturarv af UNESCO bl.a. fordi øen har været et vigtigt handelscentrum med spor fra arabiske, indiske, europæiske og afrikanske tilrejsende og bosættere. Men hvad betyder det for mul...

  14. Ambientes terrestres da ilha da Trindade, Atlântico Sul: Caracterização do solo e do meio físico como subsídio para criação de uma Unidade de Conservação

    OpenAIRE

    Clemente, Eliane de Paula

    2006-01-01

    As ilhas oceânicas brasileiras possuem grande importância ambiental, tanto do ponto de vista da biodiversidade quanto do interesse científico, em virtude do isolamento geográfico. Dentre estas, a ilha da Trindade destaca-se por ser a mais isolada e úmida do conjunto das ilhas oceânicas brasileiras. Foram estudados os principais solos que ocorreram na ilha da Trindade, com ênfase em suas características químicas, físicas, mineralógicas e micromorfológicas peculiares, nos diferentes estratos am...

  15. Gestão de Resíduos Sólidos e Turismo: O Tratamento Dado por Meios de Hospedagem e Pelo Setor Público na Ilha do Mel, PR.

    Directory of Open Access Journals (Sweden)

    Matias Poli Sperb

    2015-01-01

    Full Text Available A Ilha do Mel, reconhecido destino turístico do litoral do Estado do Paraná, Brasil, que enlaça duas áreas de preservação ambiental, passou por consideráveis alterações socioambientais e econômicas a partir da década de 1980, em função do aumento da demanda turística. O desenvolvimento do turismo possibilitou o surgimento de mais de cem hotéis e pousadas em seu território, concentração essa que se tornou um grave problema para a gestão pública dos resíduos sólidos. Neste contexto, o objetivo desta pesquisa é o de analisar a gestão dos resíduos sólidos realizada pelos meios de hospedagem e pelo setor público, bem como verificar a opinião dos empreendedores privados sobre o tratamento dado a esses resíduos, no local. Os dados primários foram coletados através de entrevistas realizadas com empresários de trinta meios de hospedagem e com representantes do setor público local. Os resultados indicam que muitas das ações de tratamento e destinação dos resíduos sólidos acontecem, em geral, pelo livre arbítrio dos proprietários. Por parte do setor público, foi verificada ausência de estruturas físicas adequadas para a gestão dos resíduos sólidos, assim como de critérios claros para o tratamento e destinação destes resíduos e a falta de orientações efetivas aos empresários. Management of Solid Waste and Tourism: The Treatment Given by Lodging Facilities and by the Public Sector in Ilha do Mel, Paraná State, Brazil - Ilha do Mel, a well-known touristic destination in the coast of Paraná, in the South of Brazil, ensnares two preservation areas and has gone through some important environmental and economic changes from the 1980s, due to the increased demand of tourists. Tourism development has caused the emergence of over 100 hotels and inns within the island, which became a serious problem to the public management of the solid waste. Based on that, this research aims to analyze how the solid waste

  16. Freqüência horária e sazonalidade de Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae na Ilha de São Luís, Maranhão, Brasil

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    Rebêlo José Manuel Macário

    2001-01-01

    Full Text Available A distribuição sazonal e horária de Lutzomyia longipalpis foi estudada nos ambientes peri e intradomiciliar de duas localidades da Ilha de São Luís, estado do Maranhão. Os 11.200 exemplares capturados foram atraídos por quatro armadilhas CDC nos anos de 1996 e 1997, mensalmente, das 18h às 6h. L. longipalpis comportou-se como uma espécie anual, tendo sido encontrada em alta freqüência em todos os meses do ano, tendendo a ser mais abundante no período chuvoso (57,2% do que no seco (42,8%. As maiores freqüências foram observadas nos meses de janeiro e abril, no período chuvoso, e em julho e novembro, no período seco. Foi encontrada a noite inteira, porém foi mais freqüente entre 18h e 22h no peridomicílio, e entre 20h e 2h no intradomicílio.

  17. Detecção de Aedes aegypti e Aedes albopictus, na zona urbana do município de Catanduva-SP, após controle de epidemia de dengue

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    Rubens Pinto Cardoso Junior

    1997-02-01

    Full Text Available Após a realização dos trabalhos de controle visando à interrupção da transmissão do vírus do dengue, iniciou-se um trabalho de monitorização de Aedes aegypti e Aedes albopictus com dois métodos de vigilância entomológica: Índice de Breteau (IB e ovitrampas. Pretendeu-se avaliar o tempo necessário para que as espécies envolvidas fossem novamente detectadas na área urbana do município de Catanduva, SP. As ovitrampas apresentaram positividade para Aedes aegypti dois meses após os trabalhos de controle, enquanto o Índice de Breteau veio a positivar-se somente no quarto mês após o término dos referidos trabalhos.After the realization of control research that had in view the transmition of dengue virus, we started to monitor two kinds of entomological vigilance, Breteau Index and ovitrap. We intended to evaluate the necessary time elapsed before Aedes sp mosquitoes were again detected at the urban area of Catanduva s town (SP. The ovitraps showed positiviness for the Aedes aegypti two months after the control research, while the Breteau Index became positive only at the fourth month after the end of the refered research.

  18. Fatores de risco da transmissão de zoonoses por costumes da população de Ilha Solteira, Brasil

    OpenAIRE

    Sánchez-Ortiz, Iván A; Leite, Maurício A

    2011-01-01

    Objetivo Determinar condições geradoras de possíveis transmissões de zoonoses com base nos costumes dos moradores de Ilha Solteira-São Paulo, para formular recomendações concretas às autoridades correspondentes para reduzir os fatores de risco. Métodos Foram realizadas 100 entrevistas dirigidas no perímetro urbano da cidade de Ilha Solteira no mês de abril de 2008. O público alvo foram pessoas maiores de idade que morassem ou trabalhassem na casa em estudo. Resultados Na pesquisa foram encont...

  19. CAPTAÇÃO DE ÁGUA POR SISTEMA FOTOVOLTAICO PARA CONSUMO HUMANO NA LOCALIDADE RURAL ILHA CAIAPÓS, SÃO ROMÃO - MINAS GERAIS

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    Samuel Alves Boitrago

    2016-07-01

    Full Text Available RESUMO: Este estudo teve como objetivo verificar a viabilidade técnica e econômica de implantação de um sistema de bombeamento utilizando a energia solar fotovoltaica para captação de água. Caracterizou-se como local do estudo a comunidade de Ilha Caiapós, situada no Norte de Minas Gerais, devido à ausência de energia elétrica para o suprimento do bombeamento de água. A localidade apresentou características favoráveis para implantação do sistema de captação utilizando a energia solar fotovoltaica, visto que há grande intensidade de sol e calor durante todo o ano. O sistema de captação estudado apresentou eficiência para atender a demanda da localidade em um horizonte de 20 anos, além de ser uma tecnologia limpa e renovável. ABSTRACT: This study aimed to verify the technical and economic implementing viability of a pumping system using photovoltaic solar energy for water abstraction. For this, the study was conducted in Caiapós Island community, located in North of Minas Gerais, and the choice of this location is due to lack of electricity supply to the water pump. This site showed favorable characteristics for capture system implantation using photovoltaic solar energy, since there is high solar and heat intensity throughout the year. Thus, the capture system studied showed efficiency to meet the local demand in a 20-year horizon, in addition to being a clean and renewable technology.

  20. Mulher, cura e pajelança em Soure (Ilha do Marajó-PA).

    OpenAIRE

    Mayra Cristina Silva Faro; UEPA

    2012-01-01

    Neste trabalho pretendo apresentar alguns resultados de uma pesquisa que vem sendo realizada desde 2009 sobre a pajelança cabocla e as mulheres pajés na cidade de Soure, Ilha do Marajó/PA. Este estudo tem como objetivos analisar práticas e saberes de cura em Soure, e observar o papel da mulher no contexto religioso e simbólico da pajelança. Compreende-se pajelança cabocla como um conjunto de crenças e práticas de cura bastante difundida na Amazônia, em que encontram-se mesclados em graus vari...

  1. Avaliação do consumo alimentar de crianças pertencentes a uma creche filantrópica na Ilha de Paquetá, Rio de Janeiro, Brasil Food intake assessment of children attending a philanthropic daycare center in the Ilha de Paquetá, Rio de Janeiro, Brazil

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    Roseane M. S. Barbosa

    2006-03-01

    Full Text Available OBJETIVOS: comparar o consumo alimentar de pré-escolares, pertencentes a uma creche filantrópica, em dois períodos (no ato da matrícula e após seis meses, com base na proposta da Pirâmide Alimentar Infantil Norte-Americana e através do Índice de Alimentação Saudável (IAS. MÉTODOS: relativamente à avaliação dietética, foi utilizada a história alimentar da criança com o responsável, no ato da matrícula; após seis meses de frequência da criança, utilizou-se o método de pesagem de alimentos (na creche e registro alimentar (na residência. Posteriormente, compararam-se as médias das porções consumidas nos dois períodos, de cada grupo alimentar, utilizando o teste "t" Student, a fim de se verificar a diferença estatística entre os grupos. Considerou-se o nível de significância de 5%. Igualmente, avaliou-se o IAS, nos dois períodos. RESULTADOS: observou-se uma diferença significativa (pOBJECTIVES: to compare the food intake of children in a philanthropic daycare center in two periods (at enrollment and following six months attendance based on the North American Food Guide Pyramid through the Healthy Eating Index (HEI. METHODS: the children eating habits were informed by the parents or custodians at enrollment and following six months of children's attendance, the food weighing technique was performed (at the daycare center and food checking (at home as well. Following, averages of the portions eaten in the two periods were compared in each of the food intake groups through the "t" Student test to determine the statistical significance between the groups, with the significance rate established at 5%. HEI for the two periods was equally assessed. RESULTS: a significant difference (p<0,05 was determined in the average of fruit and vegetable portions consumed, based on the North American Food Pyramid, and a satisfactory scoring of the Healthy Eating Index following six months of the children's attendance of the day care

  2. Infestação pelo mosquito Aedes aegypti (Diptera: Culicidae na cidade de Chapecó – SC

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    Junir Antonio Lutinski

    2013-03-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2013v26n2p143   A dengue se tornou um problema de saúde pública e vem causando preocupação aos profissionais de saúde. Este estudo visou a avaliar os fatores condicionantes para a ocorrência de dengue na cidade de Chapecó – SC. Foram analisadas as informações disponíveis no Sistema de Informações de Febre Amarela e Dengue (SISFAD e foi realizada uma pesquisa a partir de um questionário semiestruturado aplicado à população. As análises indicam a associação da infestação pelo mosquito transmissor da dengue com o lixo inadequadamente tratado e com os depósitos de captação de água da chuva, assim como com o ambiente residencial. A prevenção à dengue no município de Chapecó envolve a implantação de políticas públicas de educação ambiental em saúde para a separação e destinação adequada dos resíduos sólidos e melhorias no sistema de abastecimento público de água. Campanhas educativas voltadas aos cuidados no ambiente domiciliar também são necessárias.

  3. Moraceae da Ilha Grande, Angra dos Reis, Rio de Janeiro, Brasil. Moraceae of Ilha Grande, Angra dos Reis, Rio de Janeiro, Brazil.

    Directory of Open Access Journals (Sweden)

    Leandro Cardoso PEDERNEIRAS

    2012-06-01

    Full Text Available Este trabalho apresenta o levantamento florístico das espécies nativas de Moraceae ocorrentes na Ilha Grande, município de Angra dos Reis, Estado do Rio de Janeiro, Brasil. Com base na análise de coleções científicas, revisão da literatura e excursões a campo, foram registradas 11 espécies nativas: Brosimum guianense, Dorstenia arifolia, Ficus adhatodifolia, F. arpazusa, F. cyclophylla, F. gomelleira, F. nevesiae, F. organensis, F. pulchella, Sorocea hilarii, S. guilleminiana. Apresentamos, também, a chave de identificação para os táxons, descrições, ilustrações, informações sobre a distribuição geográfica e comentários sobre as espécies. This work presents a floristic survey of native species of Moraceae occurring in Ilha Grande, municipality of Angra dos Reis, Rio de Janeiro state, Brazil. It was based on analysis of herbarium collections, literature review and the field excursions, resulting in 11 native species: Brosimum guianense, Dorstenia arifolia, Ficus adhatodifolia, F. arpazusa, F. cyclophylla, F. gomelleira, F. nevesiae, F. organensis, F. pulchella, Sorocea hilarii, S. guilleminiana. We also present the identification key to the taxa, descriptions, illustrations, information about geographic distribution and comments on these species that enriched the results.

  4. Diversidade de bromeliáceas epífitas na Área de Proteção Ambiental Ilha do Combu, Belém, Pará, Brasil Diversity of epiphytic bromeliads in the environmental protection area of Combu Island, Belém, Pará, Brazil

    Directory of Open Access Journals (Sweden)

    Adriano Costa Quaresma

    2012-06-01

    Full Text Available Informações sobre a diversidade no nível de espécie oferecem subsídios importantes para o desenvolvimento sustentável e a conservação biológica. Neste contexto, estudos com Bromeliaceae merecem destaque, especialmente porque o grupo é importante ecologicamente, mas ainda pouco conhecido na Região Norte. Neste trabalho, foram demarcadas duas parcelas de 100 m x 100 m em uma floresta de várzea localizada na Área de Proteção Ambiental Ilha do Combu, em Belém, Estado do Pará. Cada parcela foi subdividida em oito parcelas de 50 m x 50 m, tendo todas as espécies e espécimes de bromeliáceas epífitas registradas e quantificadas. A diversidade do grupo foi calculada utilizando o índice de Shannon-Wiener. Foram registrados 1.339 indivíduos pertencentes a oito espécies e quatro gêneros. Tillandsia e Aechmea apresentaram maior riqueza. A diversidade de espécies na área foi de H= 1,10, apresentando dominância acentuada de muitos indivíduos em poucas espécies.Information about diversity at the species level offers data for sustainable development and biological conservation. In this context, studies about Bromeliaceae are noteworthy, especially because this group is ecologically important and poorly known in the North Region of Brazil. In this study, two grids (100 m x 100 m were delineated in a floodplain forest in the environmental protection area of Combu Island, Belém, Pará, Brazil. The grids were subdivided into eight grids of 50 m x 50 m, and all species and individuals of epiphytic Bromeliaceae were recorded and quantified. The diversity was calculated using the Shannon-Wiener index. A total of 1,339 individuals, belonging to eight species and four genera were recorded. Tillandsia and Aechmea presented the greatest richness. The diversity of species was H= 1.10, presenting sharp dominance of many individuals of few species.

  5. Feeding ecology of Tropidurus torquatus (Wied (Squamata, Tropiduridae in two areas with different degrees of conservation in Marambaia Island, Rio de Janeiro, Southeastern Brazil Ecologia alimentar de Tropidurus torquatus (Wied (Sauria, Tropiduridae em duas áreas com diferentes níveis de conservação na Ilha da Marambaia, Rio de Janeiro, Sudeste do Brasil

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    André L. G. de Carvalho

    2007-03-01

    Full Text Available We compared the effects of habitat disturbance on the feeding ecology of two local populations of Tropidurus torquatus (Wied, 1820 in restinga habitats on Ilha da Marambaia, Southeastern Brazil. Both populations presented a relatively diverse diet and differ in the number (D Max = 0.22, DF = 2, p = 0.00 and volume of prey consumed (D Max = 0.82, DF = 2, p = 0.00. Ants were the most frequent and numerous preys in both areas, and plant matter and coleopterans were the most important alimentary items in volume. The population from the disturbed area presented a larger niche breadth for number of prey items consumed (Bi num = 3.06 and volume (Bi vol = 2.98, when compared to the other population (Bj num = 2.44, Bj vol = 1.52. Niche overlap was higher for number of items consumed between the populations (Oij num = 0.82 but lower for volume (Oij vol = 0.05. Our data suggest dietary differences between the two lizard populations, and these differences may be associated with environmental modifications. Our main hypothesis to explain the dietary differences is based in the Optimal Foraging Theory. However, the alimentary behavior, and consequently the diet of these lizards, seems to be constrained phylogenetically, with a pattern that may have evolved in the ancestor of all iguanids.Comparamos os efeitos da degradação ambiental sobre a ecologia alimentar de duas populações de Tropidurus torquatus (Wied, 1820 em áreas de restinga da Ilha da Marambaia, sudeste do Brasil. Ambas as populações apresentaram dieta relativamente diversa e deferiram em número (D Max = 0,22; DF = 2; p = 0,00 e volume de presas consumidas (D Max = 0,82; DF = 2; p = 0,00. Formigas foram os itens mais freqüentes e numerosos em ambas as áreas, e material vegetal e coleópteros os mais importantes em volume. A população da área perturbada apresentou maior largura de nicho alimentar para número de presas consumidas (Bi num = 3,06 e volume (Bi vol = 2,98, quando comparada

  6. Impact of small variations in temperature and humidity on the reproductive activity and survival of Aedes aegypti (Diptera, Culicidae); Impacto de pequenas variacoes de temperatura e umidade na atividade reprodutiva e sobrevivencia de Aedes aegypti (Diptera, Culicidae)

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Ethiene Arruda Pedrosa de Almeida; Santos, Eloina Maria de Mendonca; Correia, Juliana Cavalcanti; Albuquerque, Cleide Maria Ribeiro de, E-mail: cleide.ufpe@gmail.co [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Centro de Ciencias Biologicas. Dept. de Zoologia

    2010-07-01

    In short space of time increase in temperature and rainfall can affect vector populations and, consequently, the diseases for them transmitted. The present study analyzed the effect of small temperature and humidity variations on the fecundity, fertility and survival of Aedes aegypti. These parameters were analyzed using individual females at temperatures ranging from 23 to 27 deg C (mean 25 deg C); 28 to 32 deg C (mean 30 deg C) and 33 to 37 deg C (mean 35 deg C) associated to 60 +- 8% and 80 +- 6% relative humidity. Females responded to an increase in temperature by reducing egg production, oviposition time and changing oviposition patterns. At 25 deg C and 80% relative humidity, females survived two-fold more and produced 40% more eggs when compared to those kept at 35 deg C and 80% relative humidity. However, in 45% of females kept at 35 deg C and 60% relative humidity oviposition was inhibited and only 15% females laid more than 100 eggs, suggesting that the intensity of the temperature effect was influenced by humidity. Gradual reductions in egg fertility at 60% relative humidity were observed with the increase in temperature, although such effect was not found in the 80% relative humidity at 25 deg C and 30 deg C. These results suggest that the reduction in population densities recorded in tropical areas during seasons when temperatures reach over 35 deg C is likely to be strongly influenced by temperature and humidity, with a negative effect on several aspects of mosquito biology. (author)

  7. Uma ilha de competência: a história do Instituto de Química Agrícola na memória de seus cientistas An island of scientific competence: a history of Brazil's Institute of Agricultural Chemistry in the memory of its scientists

    Directory of Open Access Journals (Sweden)

    Line Rodrigues de Faria

    1997-06-01

    Full Text Available Este artigo apresenta a história do IQA, mostrando sua importância para o desenvolvimento da ciência brasileira, bem como os motivos e circunstâncias históricas que provocaram sua desestruturação. A evolução desse instituto está associada às trajetórias de Walter Mors, Otto Gottlieb e Benjamin Gilbert, cujas pesquisas contribuíram para a formação de cientistas na área de química do produtos naturais. Na década de 1950, o instituto se projetou como "ilha de competência", mas foi afetado pelas mudanças ocorridas no Ministério da Agricultura e no setor agrícola nacional. A extinção desse centro de excelência não significou o fim das importantes tradições científicas que brotaram. Em grupos e instituições que surgiram em vários estados brasileiros, Mors, Gottheb a Gilbert deram continuidade às pesquisas que vinham implementando no instituto de origem.This history of Brazil's IQA, underscores the role the institution played in the development of Brazilian science as well as the historical motives and circumstances that led to its demise. The IQA's history is linked to the stories of Walter Mors, Otto Gottlieb, and Benjamin Gilbert whose research contributed to the training of scientists in the field of natural product chemistry. Although the institute earned a reputation as an "island of competence" during the 195Os, it was affected by changes within the Ministry of Agriculture and Brazil's farm sector. The closing of this center of excellence did not mean the end of important scientific traditions that blossomed there. As part of groups and institutions that emerged in different states around Brazil, Mors, Gottlieb, and Gilbert ensured the continuity of the research steadies they had been conducting within the IQA.

  8. Potencial para maricultura da carragenófita Hypnea musciformis (Wulfen J.V. Lamour. (Gigartinales - Rhodophyta na Ilha da Marambaia, Baía de Sepetiba, RJ, Brasil Mariculture potential of carragenophyta Hypnea musciformis (Wulfen J.V. Lamour. (Gigartinales - Rhodophyta on Marambaia Island, Baía de Sepetiba, Rio de Janeiro State, Brazil

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    Renata Perpetuo Reis

    2006-12-01

    Full Text Available A falta da prática de cultivo comercial de macroalgas no Brasil junto com a crescente demanda do mercado nacional de ficocolóides é suprida pela importação de algas e colheita em bancos naturais, principalmente na costa nordestina. Na busca de um cultivo em pequena escala para uso das comunidades litorâneas foi implantado um cultivo experimental com H. musciformis, na Ilha da Marambaia, RJ (43º59'35" W e 23º04'00" S. Ao todo foram testadas nove técnicas de fixação da alga nas unidades experimentais de maricultura (UEM. Nos experimentos realizados entre a primavera de 2003 e o outono de 2004, as taxas de crescimento geralmente foram negativas. Entre os invernos de 2004 e 2005, foram na maioria positivas. O fitobentos próximo à UEM foi analisado para verificar o crescimento dessa espécie na assembléia e no cultivo. Observou-se maior percentual de anfípodas e de matéria orgânica nas UEM e a presença de carposporófitos em épocas de baixa salinidade em ambos os locais. Atribuiu-se o baixo rendimento da taxa de crescimento aos fatores ambientais como: ressacas, aderência de matéria orgânica nas algas, herbivoria e danos as UEM por ação antrópica. Constatou-se que o tratamento fixando a muda com abraçadeira foi o de mais fácil manuseio e baixo custo e que somente com a conscientização da comunidade litorânea e remuneração salarial para esta atividade será possível exercer o cultivo de H. musciformis com sucesso.Lack of practice in commercial cultivation of macroalgae in Brazil allied with an increased demand for phycocolloids in the national market is met by importing algae and cropping from natural beds, especially on the Northeastern coast. Seeking to establish small scale cultivation for the use of coastal communities, experimental cultivation was implanted with H. musciformis on Marambaia Island, Rio de Janeiro (43º59'35'' W - 23º04'00'' S. Nine different techniques for fixation of algae to ropes were tested in

  9. Predação de ninhos artificiais em uma ilha na Mata Atlântica: testando o local e o tipo de ovo Artificial nest predation in Atlantic Forest Island: testing the place and the different types of egg

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    Ariane D. Alvarez

    2007-12-01

    Full Text Available Experimentos com ninhos artificiais são utilizados para testar hipóteses ecológicas e comportamentais que influenciam na predação de ninhos naturais. O tamanho do ovo, a textura da casca e a cor podem influenciar na taxa de predação, porém poucos estudos avaliam qual modelo de ovo é o mais adequado para simular a predação de ninhos em áreas tropicais. O objetivo deste trabalho foi comparar a predação de diferentes modelos de ovos (ovos de codorna, massa de modelar e canários no solo e a 1,30 m de altura no sub-bosque. O experimento foi realizado Ilha Anchieta, Ubatuba, São Paulo, Brasil. Foi encontrada uma diferença significativa na taxa de predação entre os ovos de codorna (71,87% e sintéticos (93,75% e entre os ovos de codorna e de canário (100% no solo. Entretanto, não houve diferença significativa entre os ovos sintéticos e de canário. Os ninhos no sub-bosque apresentaram um padrão diferenciado do solo quando se refere aos ovos de codorna (25% e sintéticos (28,1%, mas houve diferenças significativas quando os ovos de canário foram comparados com os ovos sintéticos e de codorna. Nosso trabalho demonstrou que diferentes tipos de ovos sobre uma mesma pressão de mesopredadores apresentaram taxas de predação diferentes. Portanto, estudos que avaliam o sucesso reprodutivo da avifauna baseado na predação de ninhos artificiais devem considerar a utilização de diferentes tipos de ovos e estratos na vegetação.Experiments on artificial nests are usually used to test ecological hypothesis and behavioural that affects the predation of natural bird nests. It is has been discussed about the size of the egg, texture and color affecting predation rate, but a few studies evaluate which egg type is more appropriate to simulate nest predation in tropical areas. The objective of this work was to compare the predation of different models of eggs (Coturnix coturnix, plasticine and Serinus canarius on the ground and understory

  10. Benefícios da atividade marítima turística de Big Game Fishing : uma análise empírica para a ilha de S. Miguel

    OpenAIRE

    Antunes, Marcos Paulo Carolino

    2016-01-01

    Dissertação de Mestrado, Ciências Económicas e Empresariais, 8 de Janeiro de 2016, Universidade dos Açores. Esta dissertação estuda os benefícios económicos da atividade Marítimo Turística Big Game Fishing na ilha de São Miguel, mais particularmente as receitas geradas, baseando-se num conjunto de inquéritos a turistas que se deslocaram à ilha para pescar, no ano de 2014. É utilizado um modelo tipo dos gastos efetuados pelos turistas, tendo variáveis específicas, usualmente utilizadas na l...

  11. Ocurrence of Vibrio spp., positive coagulase staphylococci and enteric bacteria in oysters (Crassostrea gigas harvested in the south bay of Santa Catarina island, Brazil Ocorrência de Vibrio spp., estafilococos coagulase positivo e bactérias entéricas em ostras (Crassostrea gigas cultivadas na baía sul da ilha de Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    Roberta Juliano Ramos

    2012-09-01

    Full Text Available The aim of this study was to assess the contamination of oysters (Crassostrea gigas, harvested in six different regions of the South Bay of Santa Catarina Island, with Coliforms at 45 ºC, Escherichia coli, Vibrio spp., positive coagulase staphylococci, and Salmonella sp. over a period of one year. One hundred eighty oyster samples were collected directly from their culture sites and analyzed. Each sample consisted of a pool of 12 oysters. All of the samples analyzed showed absence of Salmonella, 18 (10% samples showed presence of Escherichia coli, 15 (8.3% samples were positive for V. alginolyticus, and Vibriocholerae was detected in 4 samples (2.2%. The counts of positive-coagulase staphylococci varied from O objetivo deste estudo foi avaliar a contaminação de ostras (Crassostrea gigas cultivadas em diferentes regiões da Baía Sul da Ilha de Santa Catarina, por coliformes a 45 ºC, Escherichia coli, Vibrio spp. Estafilococos coagulase positiva e Salmonella sp., durante o período de um ano. Foram analisadas 180 amostras, coletadas diretamente no local de cultivo. Todas as amostras analisadas apresentaram ausência de Salmonella, 18 (10% amostras apresentaram presença de Escherichia coli, 15 (8,3% amostras positivas para Vibrio alginolyticus e V. cholerae foi detectado em 4 amostras (2,2%. As contagens de Estafilococos coagulase positiva variaram de <10 a 1,9 x 102 UFC.g-1, enquanto que as contagens de coliformes a 45 ºC e E. coli variaram de <3 a 1,5 x 102 NMP.g-1 e <3 e 4,3 x 10 NMP.g-1, respectivamente. As contagens de V. parahaemolyticus e V. vulnificus variaram de <3 a 7 NMP.g-1, para ambos os microrganismos, sugerindo um monitoramento tanto destas espécies quanto da temperatura das águas marinhas nas regiões de cultivo. Com base nos resultados das análises microbiológicas, as amostras analisadas mostraram qualidade bacteriológica aceitável, ou seja, dentro dos parâmetros estabelecidos na legislação brasileira.

  12. H+ V-ATPase-Energized Transporters in Brush Border Membrane Vesicles from Whole Larvae of Aedes Aegypti

    Science.gov (United States)

    Brush Border Membrane vesicles (BBMVs) from Whole larvae of Aedes aegypti (AeBBMVWs ) contain an H+ V-ATPase (V), a Na+/H+ antiporter, NHA1 (A) and a Na+-coupled, nutrient amino acid transporter, NAT8 (N), VAN for short. All V-ATPase subunits are present in the Ae. aegypti genome and in the vesicles...

  13. Bromeliad-associated mosquitoes from Atlantic forest in Santa Catarina Island, southern Brazil (Diptera, Culicidae, with new records for the State of Santa Catarina Mosquitos associados a bromélias em Mata Atlântica na Ilha de Santa Catarina, sul do Brasil (Diptera, Culicidae, com novos registros para o Estado de Santa Catarina

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    Gerson Azulim Müller

    Full Text Available Bromeliad-associated mosquitoes (Diptera: Culicidae in Atlantic Forest in Florianópolis, Santa Catarina, southern Brazil, were studied, examining plants of Vriesea philippocoburgi Wawra and Aechmea lindenii (E. Morren Baker var. lindenii at secondary Atlantic rain forest, and A. lindenii and Vriesea friburgensis Mez var. paludosa (L. B. Smith at "restinga" per month, during 12 months. No immature forms of mosquitoes were collected from A. lindenii in the secondary forest. Collections obtained 368 immature mosquitoes, none of them from A. lindenii from rain forest. Culex (Microculex spp. constituted 79.8% of the total, Wyeomyia (Phoniomyia spp. 17.93%, and Anopheles (Kerteszia cruzii (Dyar & Knab, 1908 only 1.36%. The study shows the great predominance of species of medical importance not yet proved, and the small number of immature stages of anopheline mosquitoes. The rainfall, but not the mean temperatures, significantly influenced the quantity of mosquitoes from V. philippocoburgi. Significant differences between the quantities of immature forms of all the bromeliad species were found, and the shape of the plants could be important to the abundance of mosquitoes. All six species of Cx. (Microculex found are recorded for the first time in the State of Santa Catarina, and all six species of Wyeomyia (Phoniomyia are recorded for the first time in bromeliads in this state.Mosquitos (Diptera: Culicidae associados a bromélias em Mata Atlântica na Ilha de Santa Catarina, no Estado de Santa Catarina, foram estudados. Foram examinadas mensalmente plantas de Vriesea philippocoburgi Wawra e Aechmea lindenii (E. Morren Baker var. lindenii de floresta atlântica pluvial ombrófila e A. lindenii e Vriesea friburgensis Mez var. paludosa (L. B. Smith de restinga, durante 12 meses. As coletas resultaram em 368 formas imaturas de mosquitos, sendo que nenhuma foi coletada em A. lindenii de mata ombrófila. Culex (Microculex spp. constituíram 79,8% do total

  14. Observations on the natural history of the lizard Mabuya macrorhyncha Hoge (Scincidae in Queimada Grande Island, São Paulo, Brazil Observações sobre a história natural do lagarto Mabuya macrorhyncha Hoge (Scincidae na Ilha da Queimada Grande, São Paulo, Brasil

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    Davor Vrcibradic

    2005-12-01

    Full Text Available Data are presented on the on diet, thermal biology, microhabitat use, reproduction and helminth infection parameters of a population of Mabuya macrorhyncha hoge, 1946 inhabiting the small island of Queimada Grande, on the southern coast of São Paulo State. The lizards were collected on November 1997 (spring and on July 1998 (winter. Most lizards were perched above ground, mainly on herbaceous vegetation. Mean lizard body temperatures were significantly higher in spring (32.6 ± 1.9ºC than in winter (28.5 ± 2.4ºC, as were air temperatures. Mean litter size of females was 2.7 (range 2-3. The diet was composed of a diverse array of arthropods and some small fruits. Of the 19 lizards examined, 16 (84.2% harboured helminths. Three species of helminth (one acanthocephalan and two nematodes were found infecting the lizards. Compared to other M. macrorhyncha populations previously studied in mainland areas, the Queimada Grande population appears to be less strongly associated to bromeliads, to consume plant matter (fruits more frequently, and to have a poorer helminth fauna.Dados são apresentados referentes à dieta, biologia térmica, uso de microhabitats, reprodução e parâmetros de infecção por helmintos de uma população de Mabuya macrorhyncha Hoge, 1946 habitando a pequena ilha da Queimada Grande, na costa sul do Estado de São Paulo. Os lagartos foram coletados em novembro de 1997 (primavera e em julho de 1998 (inverno. A maioria dos lagartos foi encontrada empoleirada acima do solo, principalmente sobre vegetação herbácea. As temperaturas corpóreas médias dos lagartos foram significativamente mais altas na primavera (32,6 ± 1,9ºC do que no inverno (28,5 ± 2,4ºC, como ocorreu com as temperaturas do ar. O tamanho médio de ninhada das fêmeas foi de 2.7 (amplitude 2-3. A dieta foi composta por artrópodes variados e por alguns pequenos frutos. Dos 19 lagartos examinados, 16 (84.2% estavam infectados por helmintos. Três esp

  15. Aedes albopictus (S na região de São José do Rio Preto, SP: estudo da sua infestação em área já ocupada pelo Aedes aegypti e discussão de seu papel como possível vetor de dengue e febre amarela

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    Chiaravalloti Neto Francisco

    2002-01-01

    Full Text Available Os objetivos deste trabalho foram estudar a evolução da infestação pelo Aedes albopictus na região de São José do Rio Preto, área já ocupada pelo Aedes aegypti e discutir seu papel na transmissão de doenças. Com informações obtidas em medidas de densidade larvária realizadas em áreas urbanas dos municípios, analisaram-se: ano e local de ocorrência, composição e localização das amostras larvárias; recipientes e Índices de Breteau. Até maio de 2001, o vetor já se encontrava em 96 dos 100 municípios da região. O Aedes albopictus, comparativamente ao Aedes aegypti, ocupou em maior proporção o peridomicílio e apresentou maior grau de associação com recipientes naturais e descartáveis. O comportamento endêmico do dengue, a ocorrência de casos autóctones de febre amarela silvestre na região e a reconhecida competência do vetor para estas doenças implicam em considerar a possibilidade de sua participação na transmissão do dengue e na reurbanização da febre amarela.

  16. Lemongrass Oil Granules AS Aedes Aegypti Larvicide

    OpenAIRE

    Mulyani, Sri

    2014-01-01

    One way to prevent the spread of Haemorrhage Dengue Fever is the use of abate. The use of abate as larvicides often complained causing an unpleasant smell, and can cause resistance. Lemongrass oil is reported to have activity as larvicides, and this study aims to make granules of lemongrass oil preparation, as well as determining the value of LC50, LC90 against larvae of Ae. aegypti instar III. The granules of lemongrass oil preparation are made with lactose filler and binder CMC-Na. Larvicid...

  17. O fogo e seus efeitos sobre a herpeto e a mastofauna terrestre no Parque Nacional de Ilha Grande (PR/MS), Brasil

    OpenAIRE

    Koproski, Letícia de Paulo

    2013-01-01

    O presente trabalho foi desenvolvido no Parque Nacional de Ilha Grande, Unidade de Conservação situada na região sul da planície de inundação do alto Rio Paraná, na divisa dos Estados do Paraná e Mato Grosso do Sul, Brasil. Os objetivos foram caracterizar o perfil dos incêndios, definir a estação de perigo de incêndios, descrever as atitudes dos animais frente ao fogo, identificar as espécies atingidas pelos incêndios e relacionar a natureza das lesões causadas pelo fogo, e identificar as esp...

  18. O encontro da militância com a vadiagem nas prisões da Ilha Grande

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    Myrian Sepulveda Santos

    Full Text Available RESUMO A partir do estudo sobre prisões da Ilha Grande na primeira metade do século XX, este artigo investiga a construção de uma cultura punitiva que criminalizou grupos sociais que se encontravam em condições precárias de sobrevivência. O termo “vagabundo”, responsável pela prisão e maus tratos de milhares de indivíduos no período pós-abolicionista, esteve presente em leis e códigos penais, no pensamento de legisladores e em textos ditos científicos. O encontro entre lideranças políticas, adversárias do governo Vargas, e a dita vadiagem nas prisões da Ilha Grande evidenciou tratamentos diferenciados e a naturalização do conceito. Entrevistas com guardas penitenciários da Colônia Agrícola do Distrito Federal, construída em 1942, permitem indicar como essas classificações foram operacionais no interior do sistema prisional e como elas se desdobraram.

  19. Uso de dados de Sensoriamento Remoto para identificação de ilhas de calor em Teresina - PI

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    Felipe Ferreira Monteiro

    2012-12-01

    Full Text Available As mudanças climáticas globais são uma das grandes preocupações da atualidade e em áreas urbanas o aumento de temperatura é o fenômeno mais percebido. O trabalho objetiva identificar ilhas de calor em bairros da zona leste de Teresina-PI utilizando dados do satélite Landsat-5, para o ano de 2010. Foi possível verificar a relação entre a densidade construída, o padrão de ocupação e a pouca cobertura vegetal com as áreas de maiores temperatura na área estudada. O estudo demonstrou a influência da urbanização no aumento da temperatura e a necessidade das áreas verdes para minimizar os efeitos das mudanças climáticas.

  20. Perfil Ictiofaunístico de duas Lagoas no Rio Paraná, Região do Parque Nacional de Ilha Grande - PR. = Ictiofaunistic profile of two Rio Paraná lagoons, Region of Ilha Grande National Park - PR.

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    Wladimir M. Domingues

    2008-07-01

    Full Text Available O Rio Paraná é o décimo maior do mundo em descarga e nele se encontra a região do Parque Nacional de Ilha Grande, que é formada por um conjunto de ilhas, lagoas e várzeas periodicamente alagadas, sendo estas de extrema importância para os peixes, servindo de refúgio contra predação, berçário natural e áreas de alimentação para muitas espécies de peixes. Este artigo apresenta um levantamento icitiofaunístico nas lagoas Saraiva e São João, situadas no Parque Nacional de Ilha Grande, com enfoque nas variações espaço-temporais, na composição específica e estrutura etária das assembléias de peixes, sendo que estes foram amostrados em coletas trimestrais, utilizando-se redes de espera de diferentes malhagens. Em termos de número de indivíduos capturados e proporção, tem-se 54% das espécies pertencentes a ordem Characiformes, 42% a Siruliformes, 3% a Perciformes. Rajiformes e Gymnotiformes contribuíram com menos de 1,5% do total das capturas. Na lagoa Saraiva foram capturadas 34 espécies pertencentes a cinco ordens e 17 famílias, destacando-se numericamente Loricariichthys platymetopon, Raphiodon vulpinus, Serrasalmus marginatus e Plagioscion squamosissimus. As maiores contribuições em peso foram proporcionadas por R. vulpinus, Potanotrygon motoro, P. squamosissimus e S. marginatus. Na lagoa São João ocorreram 46 espécies pertencentes a cinco ordens e 17 famílias, destacando-se em número L. platymetopon, Acestrohynchus lacustris, Serrasalmus spilopleura e S. marginatus. Em peso, Prochilodus lineatus, A. lacustris, L. platymetopon e Serrasalmus spilopleura foram as mais representativas. Destaca-se que a maioria das espécies registradas em ambas as lagoas são típicas de ambientes lênticos, utilizando esses locais para seu desenvolvimento e crescimento. = Paraná River is the tenth river in the world concerning to discharge and surrounding it Ilha Grande National Park region is localized, wich is formed by a

  1. Temporal genetic stability of Stegomyia aegypti (= Aedes aegypti) populations.

    Science.gov (United States)

    Gloria-Soria, A; Kellner, D A; Brown, J E; Gonzalez-Acosta, C; Kamgang, B; Lutwama, J; Powell, J R

    2016-06-01

    The mosquito Stegomyia aegypti (= Aedes aegypti) (Diptera: Culicidae) is the primary vector of viruses that cause yellow fever, dengue and Chikungunya fever. In the absence of effective vaccines, the reduction of these diseases relies on vector control strategies. The success of these strategies is tightly linked to the population dynamics of target populations. In the present study, 14 collections from St. aegypti populations separated by periods of 1-13 years were analysed to determine their temporal genetic stability. Although temporal structure is discernible in most populations, the degree of temporal differentiation is dependent on the population and does not obscure the geographic structure of the various populations. The results suggest that performing detailed studies in the years prior to and after population reduction- or modification-based control interventions at each target field site may be useful in assessing the probability of success. © 2016 The Royal Entomological Society.

  2. Abundância e locais de ocorrência do jacaré-de-papo-amarelo (Caiman latirostris, Alligatoridae no noroeste da Ilha de Santa Catarina, SC

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    Roberto Fusco-Costa

    2008-01-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2008v21n4p183 Este é o primeiro estudo sobre a distribuição e abundância relativa do jacaré-de-papo-amarelo na Ilha de Santa Catarina. Foi estimada a abundância relativa do jacaré ao longo dos rios na Estação Ecológica de Carijós, além de verificar a sua ocorrência em toda a planície do Rio Ratones, noroeste da ilha. A média da abundância relativa foi de 0,25 (±0,07 jacarés/km percorrido, sendo obtida através de contagem noturna. Houve uma fraca correlação do número de jacarés com a temperatura do ar. Através de entrevistas com moradores locais e levantamento noturno da espécie em açudes e rios no entorno da unidade de conservação, foi verificado que os jacarés cobrem toda a planície do Rio Ratones, podendo ser encontrados em habitats naturais (rios, banhados, manguezais e artificiais (canais de drenagem e açudes. Embora este estudo revele informações básicas sobre a distribuição do Caiman latirostris no noroeste da ilha, ele serve como base para futuras pesquisas.

  3. Rhamnolipids: solution against Aedes aegypti?

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    Vinicius Luiz Silva

    2015-02-01

    Full Text Available Aedes aegypti mosquitoes are the primary transmitters of dengue fever, urban yellow fever and chikungunya viruses. This mosquito has developed resistance to the insecticides currently used to control their populations. These chemical insecticides are harmful to the environment and can have negative effects on human health. Rhamnolipids are environmentally compatible biological surfactants, but their insecticidal activity has not been extensively studied. The present study evaluated the potential larvicidal, insecticidal and repellent activities of rhamnolipids against Aedes aegypti. At concentrations of 800, 900 and 1000 mg/L, rhamnolipids eliminated all mosquito larvae in 18 hours and killed 100% of adults at 1000 mg/L. According to the results it may be conclude that rhamnolipids should be applied to control larvae and mosquitos besides present the repellency activity against Aedes aegypti.

  4. Quantificação da ilha de calor de curitiba considerando aspectos de estabilidade atmosférica

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    Eduardo Krüger

    2015-12-01

    Full Text Available RESUMO A relação entre crescimento urbano e o surgimento de ilhas de calor urbanas, ou seja, diferenças climáticas entre a área urbana e as áreas rurais no entorno, é demonstrada por diversos autores e em diferentes regiões climáticas. Em Curitiba, a população saltou de aproximadamente 600.000 habitantes para um milhão e meio em três décadas. O objetivo do presente trabalho é propor uma metodologia de análise da ilha de calor urbana de Curitiba (25º25'40"S, 49º16'23"W, 934m acima do nível do mar. A cidade está localizada em região de clima subtropical de altitude; o monitoramento climático foi conduzido segundo uma série anual, entre dezembro de 2011 e fevereiro de 2013, utilizando um par de estações meteorológicas dentro e fora (perifericamente da mancha urbana de Curitiba. De modo a minimizar possíveis influências atmosféricas na análise, foi feita a classificação dos dias de medição por classes de estabilidade atmosférica segundo o método de Pasquill-Gifford-Turner (PGT, procedendo-se à análise para dias com maior estabilidade atmosférica. Como complemento à análise, adota-se um modelo de conforto desenvolvido para Curitiba a partir de uma extensa pesquisa de sensação e percepção térmicas em espaços abertos, de modo a verificar o efeito integrado das diversas variáveis climáticas em termos de percentuais de horas em frio, conforto e calor e suas variações dentro e fora da área urbana. Em Curitiba, a ilha de calor urbana pode ser benéfica durante o inverno, porém trazendo consequências em termos de excesso de calor em ambientes internos no verão.

  5. Situação epidemiológica da leishmaniose visceral, na Ilha de São Luís, Estado do Maranhão Epidemiological situation of visceral leishmaniasis on the Island of São Luis, State of Maranhão

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    Antonio Rafael da Silva

    2008-08-01

    Full Text Available Apresenta-se o resultado de um estudo de campo sobre a leishmaniose visceral autóctone da Ilha de São Luís. Com início em 2004 e término em 2006, a pesquisa visou conhecer aspectos epidemiológicos e clínicos determinantes da endemia. Foram analisados 299 casos autóctones, sendo 83,6% em menores de 9 anos e 54,1% do sexo masculino. O agravo ocorreu em todos os meses do ano com pico em junho. O coeficiente de incidência foi reduzido de 46,1 para 35,2 casos por 100.000 habitantes nos anos estudados. O diagnóstico teve confirmação laboratorial em 93,3% dos casos. O tratamento de escolha foi à base de N-metilglucamina com percentual de cura de 96,1%. A letalidade média foi de 3,7%. Em função da inexistência de ações mais sistemáticas de controle, propõe-se a criação de um programa a ser desenvolvido pelos municípios sob a coordenação da Secretaria de Estado da Saúde.The results from a field study on autochthonous visceral leishmaniasis on the island of São Luís are presented. This study started in 2004 and finished in 2006 and had the aim of ascertaining the determinant epidemiological and clinical characteristics of this endemic disease. Two hundred ninety nine autochthonous cases were analyzed, of which 83.6% were children younger than 9 years old and 54.1% were male. The disease occurred in all months of the year, with a peak in June. The coefficient of incidence decreased from 46.1 to 35.2 cases per 100,000 inhabitants over the years studied. The diagnosis was confirmed by laboratory tests in 93.3% of the cases. The treatment of choice was based on N-methylglucamine, with a cure rate of 96.1%. The mean lethality rate was 3.7%. Because of the absence of systematic control actions, the authors propose the creation of a specific program to be developed by the municipalities under the coordination of the State Department of Health.

  6. IDENTIFICAÇÃO DE ILHAS DE CALOR POR MEIO DE SENSORIAMENTO REMOTO: ESTUDO DE CASO NO MUNICÍPIO DE GOIÂNIA – GO/2001

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    Diego Tarley Ferreira Nascimento

    2009-06-01

    Full Text Available No presente trabalho são utilizadas técnicas de sensoriamento remoto para identificação e análise do fenômeno de ilhas de calor em Goiânia-GO, tendo como recorte temporal o ano de 2001. Foi utilizada a proposta metodológica de Monteiro (1976, do Sistema de Clima Urbano – SCU, em particular o subsistema Termodinâmico, visando proceder a uma análise do funcionamento, desempenho e organização do fenômeno de ilhas de calor. A elaboração do trabalho baseou-se na classificação da cobertura do solo e em um mapeamento do campo térmico do município de Goiânia, por meio das imagens das bandas 3, 4, 5 e 6 (banda termal do Landsat 7, nas quais foram considerados como instrumentos de análise: a urbanização, as características geoecológicas (topografia e o sistema meteorológico (clima regional. Os resultados demonstram que o fenômeno de ilha de calor é constatado no município de Goiânia, havendo diferenças de temperatura de até 10°C entre a área urbana e a rural do município, sendo possível caracterizar determinados usos antrópicos (classes de cobertura do solo e intervalos altimétricos como potencializadores de maiores valores de temperatura.

  7. Distribuição vertical e sazonal de Anopheles (Kerteszia em Ilha Comprida, SP Vertical and seasonal distribution of Anopheles (Kerteszia in Ilha Comprida, Southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Helene Mariko Ueno

    2007-04-01

    Full Text Available OBJETIVO: Anopheles bellator e An. cruzii são vetores da malária-bromélia, característicos de áreas preservadas. O objetivo do estudo foi analisar a distribuição sazonal e vertical desses anofelinos, visando contribuir para a avaliação do risco de transmissão desse agravo. MÉTODOS: No município de Ilha Comprida, SP, foram instaladas armadilhas luminosas tipo CDC iscadas com gelo seco, das 17 às 20h, a um, seis e 12m do solo, quinzenalmente, de setembro de 2001 a setembro de 2002. A relação da densidade desses anofelinos com o clima foi avaliada pelo coeficiente de Spearman e suas densidades entre os estratos foram comparadas pelo teste não paramétrico de Mann-Whitney. RESULTADOS: Do total de 55.226 mosquitos, 1.341 eram An. bellator (2,4% do total de Culicidae, sendo capturados 278 indivíduos próximo ao solo, 261 a seis metros e 802 na copa (12 m. Na mesma ordem, foram capturados 452, 1.032 e 4.420 adultos de An. cruzii, totalizando 5.904 mosquitos desta espécie (10,7%. Houve correlação positiva entre a densidade das duas espécies com a temperatura máxima, e entre An. bellator e amplitude térmica diária. As densidades das duas espécies foram maiores na copa. Nos três estratos, An. cruzii predominou sobre An. bellator. CONCLUSÕES: Confirmou-se o comportamento acrodendrófilo dessas espécies, principalmente An. cruzii. Após medidas drásticas de controle, esses anofelinos permaneceram na região mas, atualmente, não são alvo de vigilância entomológica. A persistência da transmissão de malária autóctone e o incremento de atividades de ecoturismo na região apontam a necessidade de monitoramento dessas espécies.OBJECTIVE: Anopheles bellator e An. cruzii are vectors of the so-called bromeliad-malaria, typical of conserved areas. The objective was to evaluate the seasonal and vertical distribution of these anophelines, aiming at assessing the risk of malaria transmission. METHODS: In the municipality of Ilha

  8. Uso de métodos quimiométricos e mecânico-quanticos na análise de terpenóides e fenilpropanóides bioativos contra o Aedes aegypti

    Directory of Open Access Journals (Sweden)

    Reginaldo Bezerra dos Santos

    2009-12-01

    Full Text Available Dengue fever is one of the main public health problems in the world. Many mosquitoes have developed resistance to the conventional insecticides used. Thus, the search for vegetable extracts and natural substances as alternative insecticides has increased. In this study, chemometric methods were employed to classify a group ofterpenoid and phenylpropanoid compounds with biological activity against the larval of the A. aegypti mosquitoes. The AM1 (Austin Model 1 method was used to calculate a set of molecular descriptors (properties for the studied compounds. Then, the descriptors were analyzed using the following methods of pattern recognition: Principal Component Analysis (PCA and Hierarchical Clustering Analysis (HCA. The PCA and HCA methods have shown to be very effective for the classification of the study compounds in two groups (active and inactive. The electronic variables EHOMO-1, EHOMO-2, ELUMO, ELUMO+2, and the structural LogP were used to classify as active and inactive compounds. In most studied compounds, the variables responsible for separating active from inactive compounds were electronic descriptors. Thus, it can be concluded that electronic effects play a fundamental role in the interaction between biological receptor and terpenoid and phenylpropanoid compounds with activity against larval A. aegypti mosquitoes.

  9. [Zoonosis transmission risk factors according to population habits inIlha Solteira city, Brazil].

    Science.gov (United States)

    Sánchez-Ortiz, Iván A; Leite, Maurício A

    2011-06-01

    Determining conditions regarding possible zoonosis transmission risk based on Ilha Solteira-São Paulo citizens' habits aimed at establishing concrete recommendations for the corresponding local authorities to reduce some risk factors. 100 focalized interviews were held on Ilha Solteira's urban perimeter during April 2008. The people interviewed were adults who lived or worked in houses in the study area. This research found a significant number of cat and/or dog owners who allowed their pets to stay in internal areas of their houses. They did not define a specific place for animals to defecate and/or urinate or did not arrange appropriate final disposal of such waste. Local authorities must make greater efforts at educating Ilha Solteira pets' owners and providing them with information and encouraging greater citizen commitment and awareness to improve habits related to caring for pets/animal sand reducing zoonosis transmission risk factors.

  10. Influência da educação artística na preservação e proteção ambiental : investigação-ação turmas do 5º e 6º ano, Ilha de São Vicente

    OpenAIRE

    Ramos, Luísa Maria Soares Boaventura

    2018-01-01

    Dissertação de Mestrado em Educação Artística apresentada na Escola Superior de Educação do Instituto Politécnico de Viana do Castelo Numa sociedade cada vez mais globalizada e a atravessar desafios constantes na área do ambiente, é desejável que o currículo que os alunos desenvolvem na escola englobe temáticas que contribuam para uma efetiva educação ambiental. Para implementar este estudo, utilizou-se o método qualitativo, baseado nos trabalhos produzidos pelos alunos, centrado nas práti...

  11. Etapas De Construção De Uma Ilha Interdisciplinar De Racionalidade Sobre Alimentação Saudável

    Directory of Open Access Journals (Sweden)

    Ariane Pegoraro Nuncio

    2016-12-01

    Full Text Available O presente artigo tece uma reflexão sobre a interdisciplinaridade no contexto escolar e apresenta etapas de construção de uma Ilha Interdisciplinar de Racionalidade (IIR, à luz de Fourez (1994, e estratégias aplicáveis em cada uma das etapas que a compõe. A temática social é a “Alimentação saudável – o perigo do consumo excessivo de sal” em nível de ensino fundamental, na disciplina de Ciência, com o intuito de promover a alfabetização científica e mudança de hábitos visando uma melhoria na qualidade de vida, possibilitando a autonomia, o domínio e a comunicação dos estudantes.

  12. SENTIDOS SUBJETIVOS RELACIONADOS COM A MOTIVAÇÃO DOS ESTUDANTES DO CLUBE DE CIÊNCIAS DA ILHA DE COTIJUBA

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    José Moysés Alves

    Full Text Available Objetivamos, no presente estudo, conhecer os sentidos subjetivos que afetam a motivação dos estudantes do Clube de Ciências da Ilha de Cotijuba. Entrevistamos 6 estudantes sobre suas atividades, o projeto que estavam realizando, suas relações com os professores, colegas, familiares, e sobre suas aulas de Ciências na escola. Os estudantes mencionaram qualidades de suas relações interpessoais e características das atividades que os motivavam a participar do Clube e das aulas de Ciências na escola. Também apontaram problemas infraestruturais e circunstanciais que desencorajavam tal participação. Os aspectos destacados, alguns específicos desse contexto histórico-cultural, aparecem em configurações subjetivas ligeiramente diferentes para cada estudante. O enfoque adotado nos pareceu adequado para compreender a motivação dos estudantes de uma forma sistêmica.

  13. Reserva biológica estadual da Praia do Sul (Ilha Grande, Estado do Rio de Janeiro: lista preliminar da flora

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    Dorothy Sue Dunn de Araujo

    1987-12-01

    Full Text Available A Reserva Biológica Estadual da Praia do Sul está localizada na parte meridional da Ilha Grande - RJ (23º10' S.; 44º17' W.Gr., abrangendo uma área de 3600 ha. Foram identificadas nesta Reserva 5 comunidades vegetais, a saber: psamofila reptante de anteduna, mata de cordão arenoso, mata alagadiça de planície, manguezal e mata de encosta. A fitofisionomia de cada comunidade está descrita, incluindo as espécies mais comuns e a área da Reserva ocupada por cada uma. O levantamento florístico ainda incompleto constitui uma lista de cerca de 300 espécies: as 4 famílias mais importantes são: Leguminose, Rubiaceae, Orchidaceae, BromeliaceaeThe Praia do Sul State Biological Reserve is located on Ilha Grande, State oí Rio de Janeiro, Brazil (23º10' S.; 44º17' W.Gr.. Five plant communities were identified within the limits of this 3600 ha tract: creeping foredune psammophytes, beach ridge forest, coastal plain swamp forest, mangrove and montane forest. The physiognomy of each community is described, including the most common species found and the area of occurrence within the Reserve. A list of approximately 300 species has been compiled to date; the 4 most important families are: Leguminosae, Rubiaceae, Orchidaceae, Bromeliaceae

  14. ANÁLISE DA EVOLUÇÃO DO FENÔMENO DE ILHAS DE CALOR NO MUNICÍPIO DE GOIÂNIA/GO (1986-2010

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    Diego Tarley Ferreira Nascimento

    2011-12-01

    Full Text Available O processo de crescimento urbano acelerado e desorganizado tem desencadeado uma gama de impactosambientais. Dentre estes, ressalta-se a alteração do clima local, no qual o comportamento espacial simultâneoda temperatura se revela como o mais importante elemento, tendo como principal consequência a formaçãodo fenômeno de “ilhas de calor”. Nesse contexto, o presente artigo analisa a evolução das ilhas de calorno município de Goiânia/GO, tendo como recorte temporal o período de 1986 a 2010, valendo-se de dadosde sensoriamento remoto, para estimar os valores de temperatura superficial terrestre, e de técnicas degeoprocessamento, para análise e mensuração do fenômeno. Com base nos dados e nas análises realizadas,pôde-se constatar que as temperaturas superficiais terrestres apresentaram certo padrão de variaçãoespacial e, sobretudo, temporal, haja vista ter-se constatado uma verdadeira inversão na representatividadedas classes de temperatura superficial no decorrer dos anos de 1986 a 2010, que oscilaram do predomínioabsoluto das temperaturas abaixo de 24º C, para o domínio amplo das temperaturas acima desse patamar.

  15. Workbook on Identification of Aedes Aegypti Larvae.

    Science.gov (United States)

    Pratt, Harry D.; And Others

    This self-instructional booklet is designed to enable yellow fever control workers to identify the larvae of "Aedes aegypti." The morphological features of mosquito larvae are illustrated in this partially programed text, and the distinguishing features of "A. aegypti" indicated. A glossary is included. (AL)

  16. IDENTIFIKASI AEDES AEGYPTI DAN AEDES ALBOPICTUS

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    Diah Fitri Rahayu

    2013-09-01

    Full Text Available ABSTRAK. Demam Berdarah Dengue (DBD adalah penyakit yang disebabkan oleh virus Dengue yangditularkan oleh nyamuk . Kabupaten Banjarnegara merupakan daerah endemis reseptif DBD. Dari tahun ketahun kasus DBD cenderung meningkat, terutama di tahun 2009 - 2010. Vektor DBD di Kabupaten Banjarnegaraadalah Ae. aegypti dan Ae. albopictus. Ae. aegypti secara makroskopis terlihat sama seperti Ae. albopictus,namun perbedaannya terletak pada morfologi kepala (mesonotum di mana Ae. aegypti memiliki gambar garisseperti kepala kecapi berbentuk dengan dua garis lengkung dan dua garis lurus putih sementara Ae. albopictushanya memiliki satu garis putih di mesonotum tersebut.Kata kunci: identifikasi, Ae. aegypti, Ae. albopictusABSTRACT. Dengue hemorrhagic fever (DHF is a disease caused by the dengue virus transmitted by mosquito.Banjarnegara district is a Dengue receptive endemic area. From year to year the Dengue cases is likely toincrease, especially in 2009 - 2010. The main vector of dengue in Banjarnegara district is Aedes aegypti andAedes albopictus. Ae. aegypti morphologically look like with Ae. albopictus, but the difference lies in the headmorphology (mesonotum where A. aegypti has a picture of the line like a lyre-shaped head with two curved linesand two white straight line while A. albopictus has only one white stripe on the mesonotum.Key words:identification, Ae. aegypti, Ae. albopictus

  17. Degree of threat to the biological diversity in the Ilha Grande State Park (RJ and guidelines for conservation Parque Estadual da Ilha Grande -- ameaças ambientais e diretrizes para conservação

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    C. J. R. ALHO

    2002-08-01

    Full Text Available The State Park of Ilha Grande is only a part (5,594 hectares of the entire island (19,300 hectares which is located off the south coast of Rio de Janeiro state, between the cities of Mangaratiba and Angra dos Reis. Approximately half of the Park area (47% is covered by dense Atlantic forest. The secondary forest growth is in a process of ecological succession close to attaining maturity (43% and the remaining part (10% is composed of human-altered areas (1%, rocky outcrops with herbaceous vegetation (7%, mangroves and beaches (2%. The fauna is well represented but already shows signs of degradation with introduced species. The analysis of the degree of threat has shown that the dense forest habitat has a relatively stable status of conservation while the secondary forest, the mangrove and the herbaceous vegetation on rocky outcrops (and their fauna are categorized as vulnerable. The area altered by human occupation is considered threatened. Since the coastal area where Ilha Grande is located is well known for its beautiful scenery (known as the green coast, because of the contrast between the ocean and the Atlantic forest covering the Serra do Mar mountain chain. There is a strong possibility for tourism to become the means in which to achieve economic sustainability for conservation. Contradictorily, tourism is also the major threat to local biodiversity and its landscape units. Because tourism is not organized and controlled, during high season the numbers grow above local capacity, giving rise to a proliferation of hotels, guesthouses and camping grounds. The resulting untreated open sewage, random garbage disposal and other harmful activities form the major threats to biodiversity.O Parque Estadual da Ilha Grande é somente uma parte (5.594 hectares de toda a ilha (19.300 hectares localizada na costa sul do Estado do Rio de Janeiro, entre as cidades de Mangaratiba e Angra dos Reis. Aproximadamente a metade da área do Parque (47% é coberta

  18. Global genetic diversity of Aedes aegypti.

    Science.gov (United States)

    Gloria-Soria, Andrea; Ayala, Diego; Bheecarry, Ambicadutt; Calderon-Arguedas, Olger; Chadee, Dave D; Chiappero, Marina; Coetzee, Maureen; Elahee, Khouaildi Bin; Fernandez-Salas, Ildefonso; Kamal, Hany A; Kamgang, Basile; Khater, Emad I M; Kramer, Laura D; Kramer, Vicki; Lopez-Solis, Alma; Lutomiah, Joel; Martins, Ademir; Micieli, Maria Victoria; Paupy, Christophe; Ponlawat, Alongkot; Rahola, Nil; Rasheed, Syed Basit; Richardson, Joshua B; Saleh, Amag A; Sanchez-Casas, Rosa Maria; Seixas, Gonçalo; Sousa, Carla A; Tabachnick, Walter J; Troyo, Adriana; Powell, Jeffrey R

    2016-11-01

    Mosquitoes, especially Aedes aegypti, are becoming important models for studying invasion biology. We characterized genetic variation at 12 microsatellite loci in 79 populations of Ae. aegypti from 30 countries in six continents, and used them to infer historical and modern patterns of invasion. Our results support the two subspecies Ae. aegypti formosus and Ae. aegypti aegypti as genetically distinct units. Ae. aegypti aegypti populations outside Africa are derived from ancestral African populations and are monophyletic. The two subspecies co-occur in both East Africa (Kenya) and West Africa (Senegal). In rural/forest settings (Rabai District of Kenya), the two subspecies remain genetically distinct, whereas in urban settings, they introgress freely. Populations outside Africa are highly genetically structured likely due to a combination of recent founder effects, discrete discontinuous habitats and low migration rates. Ancestral populations in sub-Saharan Africa are less genetically structured, as are the populations in Asia. Introduction of Ae. aegypti to the New World coinciding with trans-Atlantic shipping in the 16th to 18th centuries was followed by its introduction to Asia in the late 19th century from the New World or from now extinct populations in the Mediterranean Basin. Aedes mascarensis is a genetically distinct sister species to Ae. aegypti s.l. This study provides a reference database of genetic diversity that can be used to determine the likely origin of new introductions that occur regularly for this invasive species. The genetic uniqueness of many populations and regions has important implications for attempts to control Ae. aegypti, especially for the methods using genetic modification of populations. © 2016 John Wiley & Sons Ltd.

  19. Global Genetic Diversity of Aedes aegypti

    Science.gov (United States)

    Gloria-Soria, Andrea; Ayala, Diego; Bheecarry, Ambicadutt; Calderon-Arguedas, Olger; Chadee, Dave D.; Chiappero, Marina; Coetzee, Maureen; Elahee, Khouaildi bin; Fernandez-Salas, Ildefonso; Kamal, Hany A.; Kamgang, Basile; Khater, Emad I. M.; Kramer, Laura D.; Kramer, Vicki; Lopez-Solis, Alma; Lutomiah, Joel; Martins, Ademir; Micieli, Maria Victoria; Paupy, Christophe; Ponlawat, Alongkot; Rahola, Nil; Rasheed, Syed Basit; Richardson, Joshua B.; Saleh, Amag A.; Sanchez-Casas, Rosa Maria; Seixas, Gonçalo; Sousa, Carla A.; Tabachnick, Walter J.; Troyo, Adriana; Powell, Jeffrey R.

    2016-01-01

    Mosquitoes, especially Aedes aegypti, are becoming important models for studying invasion biology. We characterized genetic variation at 12 microsatellite loci in 79 populations of Ae. aegypti, from 30 countries in six continents and used them to infer historical and modern patterns of invasion. Our results support the two subspecies Ae. aegypti formosus and Ae. aegypti aegypti as genetically distinct units. Ae. aegypti aegypti populations outside Africa are derived from ancestral African populations and are monophyletic. The two subspecies co-occur in both East Africa (Kenya) and West Africa (Senegal). In rural/forest settings (Rabai District of Kenya) the two subspecies remain genetically distinct whereas in urban settings they introgress freely. Populations outside Africa are highly genetically structured likely due to a combination of recent founder effects, discrete discontinuous habitats, and low migration rates. Ancestral populations in sub-Saharan Africa are less genetically structured, as are the populations in Asia. Introduction of Ae. aegypti to the New World coinciding with trans-Atlantic shipping in the 16th to 18th Centuries was followed by its introduction to Asia in the late 19th Century from the New World or from now extinct populations in the Mediterranean Basin. Aedes mascarensis is a genetically distinct sister species to Ae. aegypti s.l.. This study provides a reference database of genetic diversity that can be used to determine the likely origin of new introductions that occur regularly for this invasive species. The genetic uniqueness of many populations and regions has important implications for attempts to control Ae. aegypti, especially for methods using genetic modification of populations. PMID:27671732

  20. A construção da autoridade entre os donos de barco no Aventureiro, Ilha Grande - RJ: uma etnografia das relações de poder

    Directory of Open Access Journals (Sweden)

    Gustavo Villela Lima da Costa

    2011-12-01

    Full Text Available Este artigo propõe analisar a articulação entre as esferas de poder local e o poder público, a partir de uma descrição etnográfica da organização social e de como, nesta, se estrutura a autoridade política local. Nesse processo de construção da autoridade local, na praia do Aventureiro, Ilha Grande - RJ, o poder político e a autoridade legitimada pelos moradores estão diretamente ligados à posse dos barcos e dos campings mais prósperos. Os donos de barco no Aventureiro assumem, nesse contexto, o papel de representantes legítimos do povoado na negociação com órgãos da Prefeitura e do Estado, na defesa dos direitos dos moradores, assumindo um papel de representação da organização doméstica (em famílias e grupos vicinais nas relações políticas com o Estado.This paper proposes, from an ethnographic description of social organization and how it structures the local political authority, to analyze the connections between local power and public administration through the building of local authority at Aventureiro Beach, Ilha Grande, state of Rio de Janeiro. The inhabitants' political power and legitimate authority are connected to the ownership of boats and to the most successful campings. The boat owners take the role of legitimate at the negotiation with the municipality and state, looking after the inhabitants' rights, assuming a role in representing the domestic organization (families and vicinal groups in political relations with the state.

  1. O CONTEXTO NORDESTINO NOS CORDÉIS O BALÃO DO DESTINO OU A MENINA DA ILHA

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    Luciana Almeida dos Santos

    2014-04-01

    Full Text Available

    A literatura de cordel possui um histórico cultural que abrange desde a poesia oral até os folhetos impressos que tratam de romances, pelejas, acontecimentos históricos, tragédias, narrativas fantásticas sobre reinos, príncipes, princesas, fadas e bruxas, entre outros. Os folhetos, embora sejam considerados universais, apresentam aspectos caracterizadores de uma realidade particular, muitas vezes marcada pelo contexto nordestino, local onde a literatura de cordel se consolidou no Brasil. Os cordéis O balão do destino ou a menina da ilha, apesar de tratarem de uma história de amor situada em um reino, remete ao imaginário do povo nordestino, ao retratar, sobretudo, os costumes do seu povo e sua religiosidade. Os valores morais e éticos do povo do nordeste também permeiam este folheto. O cordelista regionaliza alguns elementos presentes na narrativa, a fim de aproximar o leitor/ouvinte do universo da literatura de cordel.

  2. The Ilha Anchieta Quartz Monzonite: the southernmost expression of ca. 500 Ma post-collisional magmatism in the Ribeira Belt

    Directory of Open Access Journals (Sweden)

    José M. Azevedo Sobrinho

    2011-09-01

    Full Text Available The Ilha Anchieta Quartz Monzonite (IAQM occupies most of the homonymous island in the coast of the state of São Paulo, and is intrusive into foliated rocks of the ~565 Ma Ubatuba Charnockite. The main petrographic variety is a porphyritic biotite-hornblende quartz monzonite with 2-4 cm tabular microcline megacrysts set in a medium-grained groundmass and magmatic foliation. Outcrop-scale structures indicate cumulative processes (modal and grain-size magmatic banding and interaction with basic magmas (mafic microgranular enclaves. Lithogeochemical data indicates that the main variety is intermediate to acid (S1O2 = 63-67%, alkali-calcic, metaluminous and magnesian (mg# ~30, showing moderate Sr (300-400 ppm and Ba (~1500 ppm contents and relatively high HFSE (Nb = 40 ppm; Zr = 550-700 ppm. The older charnockites are more silicic (S1O2 = 71-78%, ferroan(mg# = 12-16, and have very low Sr (13-80 ppm contents, resulting in Ba/Sr ratios remarkably higher than the IAQM (10 versus 4. LA-MC-ICPMS U-Pb zircon dating of the IAQM yielded 499.7 ± 5.9 Ma. This is the youngest magmatic age identified so far in the crystalline basement of the state of São Paulo, and indicates that the pluton is the southernmost expression of the post-collisional "G5" magmatism in the Ribeira Belt.O Quartzo Monzonito Ilha Anchieta (QMIA ocupa a maior parte da ilha homônima na região costeira do Estado de São Paulo, e é intrusivo em rochas foliadas do Charnockito Ubatuba (~565 Ma. A principal variedade petrográfica é um biotitahornblenda quartzo monzonito porfirítico com foliação magmática e megacristais tabulares de microclínio com 2-4 cm em matriz de granulação média. Estruturas em afloramento indicam processos cumuláticos (bandamento modal e granulométrico e interação com magmas básicos (enclaves microgranulares máficos. Dados geoquímicos indicam que a variedade principal é intermediária a ácida (SiO2 = 63-67%, tem caráter

  3. História da literatura infantil na Madeira: reflexos de um mundo em mudança

    OpenAIRE

    Ramos, Dina Isabela Lemos

    2011-01-01

    O aumento substancial do número de livros infantis publicados na Madeira, ou produzidos por autores nativos da Ilha, mais cedo ou mais tarde, daria como resultado a escrita de um ensaio teórico acerca deste assunto. Esta é a essencial finalidade deste trabalho. Percorrendo diversas matérias em redor da Literatura Infantil pretende-se dar uma ideia da sua evolução e crescimento na Ilha. Através de uma contextualização inicial do género Literatura Infantil, fornecem-se diversos dados acerca de...

  4. Dominância de Aedes aegypti sobre Aedes albopictus no litoral sudeste do Brasil Dominance of Aedes aegypti over Aedes albopictus in the southeastern coast of Brazil

    Directory of Open Access Journals (Sweden)

    Ricardo A Passos

    2003-12-01

    Full Text Available OBJETIVO: Analisar a infestação de Aedes aegypti e Aedes albopictus e verificar sua associação com fatores climáticos e com a sua freqüência em recipientes de área urbana. MÉTODOS: Foi selecionado o município de São Sebastião, localizado no litoral Sudeste do Brasil. Foram utilizados os dados do "Programa de Controle de Vetores de Dengue e Febre Amarela no Estado de São Paulo" que realiza a vigilância entomológica em pontos estratégicos, armadilhas e delimitação de focos. Os pontos estratégicos são imóveis onde existem recipientes em condições favoráveis à proliferação de larvas. Para análise dos dados, foram utilizados os testes de significância estatística: Kruskal-Wallis, Dwass-Steel-Chritchlow-Fligne e Mann-Whitney. RESULTADOS: Verificou-se crescimento anual da positividade de armadilhas e pontos estratégicos para Ae. aegypti e diminuição para Ae. albopictus. Observou-se aumento do número de imaturos de Ae. aegypti e diminuição da outra espécie. Na positividade de imóveis para a presença de larvas, verificou-se aumento gradativo do número de imóveis com Ae. Aegypti, superando a positividade para Ae. albopictus. Houve uma fraca correlação das espécies com os fatores abióticos. As maiores freqüências de imaturos de ambas espécies foram em recipientes artificiais passíveis de remoção. CONCLUSÕES: Os resultados evidenciaram no período de estudo a predominância de Ae. aegypti sobre Ae. albopictus em área urbana, indicando que o crescimento populacional do primeiro parece ter afetado a chance de sua coexistência. Sugere-se que algum processo de seleção natural possa estar operando e desse modo contribuindo para levar à separação das duas espécies.OBJECTIVE: To assess infestation of Aedes aegypti and Aedes albopiticus and describe their association with weather conditions and container colonization in urban areas. METHODS: The town of São Sebastião in the southeastern coast of Brazil

  5. Retalho em ilha de tubarão: uma técnica cirúrgica reconstrutiva de defeitos localizados na área nasal alar/perialar. Um procedimento simples Shark island pedicle flap for repairing of basal cell carcinoma localized in nasal ala-perialar region: a simple procedure

    Directory of Open Access Journals (Sweden)

    Marisa C André

    2011-08-01

    Full Text Available O Carcinoma Basocelular é a neoplasia cutânea mais frequente. Os autores descrevem uma técnica realizada unicamente num tempo operatório para correção de defeitos na asa do nariz após excisão tumoral. Esta técnica simples permite a correção cirúrgica de defeitos nesta localização possibilitando a reconstrução da anatomia local e a preservação das unidades cosméticas, sem a necessidade de enxertoBasal Cell Carcinoma is the most common skin cancer. We describe a single-staged technique for correction of nasal ala defect after the excision of a basal cell carcinoma. This technique allows correction of surgical defects of the ala rebuilding the original anatomy, maintaining cosmetic units, without need for a graft

  6. Distribution of Voltage-Gated Sodium Channel (Nav) Alleles among the Aedes aegypti Populations In Central Java Province and Its Association with Resistance to Pyrethroid Insecticides.

    Science.gov (United States)

    Sayono, Sayono; Hidayati, Anggie Puspa Nur; Fahri, Sukmal; Sumanto, Didik; Dharmana, Edi; Hadisaputro, Suharyo; Asih, Puji Budi Setia; Syafruddin, Din

    2016-01-01

    The emergence of insecticide resistant Aedes aegypti mosquitoes has hampered dengue control efforts. WHO susceptibility tests, using several pyrethroid compounds, were conducted on Ae. aegypti larvae that were collected and raised to adulthood from Semarang, Surakarta, Kudus and Jepara in Java. The AaNaV gene fragment encompassing kdr polymorphic sites from both susceptible and resistant mosquitoes was amplified, and polymorphisms were associated with the resistant phenotype. The insecticide susceptibility tests demonstrated Ae, aegypti resistance to the pyrethroids, with mortality rates ranging from 1.6%-15.2%. Three non-synonymous polymorphisms (S989P, V1016G and F1534C) and one synonymous polymorphism (codon 982) were detected in the AaNaV gene. Eight AaNaV alleles were observed in specimens from Central Java. Allele 3 (SGF) and allele 7 (PGF) represent the most common alleles found and demonstrated strong associations with resistance to pyrethroids (OR = 2.75, CI: 0.97-7.8 and OR = 7.37, CI: 2.4-22.5, respectively). This is the first report of 8 Ae. aegypti AaNaV alleles, and it indicates the development of resistance in Ae. aegypti in response to pyrethroid insecticide-based selective pressure. These findings strongly suggest the need for an appropriate integrated use of insecticides in the region. The 989P, 1016G and 1534C polymorphisms in the AaNaV gene are potentially valuable molecular markers for pyrethroid insecticide resistance monitoring.

  7. Comunidades de morcegos em hábitats de uma Mata Amazônica remanescente na Ilha de São Luís, Maranhão Bat communities of an Amazonian remnant forest of São Luís Island, Maranhão

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    Leonardo Dominici Cruz

    2007-01-01

    Full Text Available Estudos com comunidades de morcegos são escassos no Brasil, não sendo encontrado nenhum no Maranhão. Este estudo teve como objetivo investigar a composição de espécies da comunidade de morcegos do Parque Estadual do Bacanga (PEB, São Luís - MA, além de contribuir para o levantamento da fauna de morcegos do estado. Os morcegos foram capturados com três redes neblina de maio a agosto de 2004 em quatro diferentes hábitats (mata de capoeira, mata de terra firme, mata de várzea e mangue. A diversidade e similaridade entre hábitats foram calculadas, bem como a amplitude e sobreposição dos nichos das espécies consideradas comuns na área. Foram registradas 24 espécies de morcegos, sendo a maior diversidade encontrada na mata de várzea. A baixa similaridade constatada entre o hábitat de mangue e os demais hábitats indica a existência de dois conjuntos de espécies de morcegos distintos, o que é reforçado pelas sobreposições de nicho espacial das espécies mais comuns.There are few studies about bat communities in Brazil and none for the Maranhão state there is none. The present study aimed to analyze the bat community of the Bacanga State Park (PEB, São Luís - MA, and to contribute with bat data for the Maranhão state. The bats were sampled in four different habitats (capoeira forest, terra firme forest, riparian forest, and mangrove, using three mist nets from May to August 2004. Diversity and similarity were calculated, as well as the spatial niches width and overlap of the commonest species in PEB. Twenty four species of bats were recorded in PEB and the riparian habitat forest presented the highest diversity index. The low similarity found between mangrove and the remaining habitats seemed to identify two well-defined assemblages of bats in the sampled area, which is enforced by the spatial niche overlaps of the most commonness species.

  8. Era uma vez uma ilha de Pescadores Artesanais: impactos socioambientais dos grandes complexos industriais, conflitos e resistência (Ilha da Madeira/Itaguaí/RJ)

    OpenAIRE

    Vera de Fátima Maciel Lopes

    2013-01-01

    O presente estudo visa analisar os processos de transformações econômicos, políticos e socioambientais decorrentes da instalação dos grandes empreendimentos em territórios tradicionais da pesca, mais especificamente, as experiências da comunidade pesqueira da Ilha da Madeira/baía de Sepetiba/Itaguaí-RJ, desde a instalação da Cia Ingá Mercantil (1964) até os dias atuais, identificando, nos vários ciclos de industrialização: os fatores endógenos e exógenos que contribuem para a vulnerabilidade ...

  9. Dispersal of Engineered Male Aedes aegypti Mosquitoes.

    Science.gov (United States)

    Winskill, Peter; Carvalho, Danilo O; Capurro, Margareth L; Alphey, Luke; Donnelly, Christl A; McKemey, Andrew R

    2015-11-01

    Aedes aegypti, the principal vector of dengue fever, have been genetically engineered for use in a sterile insect control programme. To improve our understanding of the dispersal ecology of mosquitoes and to inform appropriate release strategies of 'genetically sterile' male Aedes aegypti detailed knowledge of the dispersal ability of the released insects is needed. The dispersal ability of released 'genetically sterile' male Aedes aegypti at a field site in Brazil has been estimated. Dispersal kernels embedded within a generalized linear model framework were used to analyse data collected from three large scale mark release recapture studies. The methodology has been applied to previously published dispersal data to compare the dispersal ability of 'genetically sterile' male Aedes aegypti in contrasting environments. We parameterised dispersal kernels and estimated the mean distance travelled for insects in Brazil: 52.8 m (95% CI: 49.9 m, 56.8 m) and Malaysia: 58.0 m (95% CI: 51.1 m, 71.0 m). Our results provide specific, detailed estimates of the dispersal characteristics of released 'genetically sterile' male Aedes aegypti in the field. The comparative analysis indicates that despite differing environments and recapture rates, key features of the insects' dispersal kernels are conserved across the two studies. The results can be used to inform both risk assessments and release programmes using 'genetically sterile' male Aedes aegypti.

  10. Spatial distribution and abundance of nonindigenous coral genus Tubastraea (Cnidaria, Scleractinia around Ilha Grande, Brazil

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    A. F. Paula

    Full Text Available The distribution and abundance of azooxanthellate coral Tubastraea Lesson, 1829 were examined at different depths and their slope preference was measured on rocky shores on Ilha Grande, Brazil. Tubastraea is an ahermatypic scleractinian nonindigenous to Brazil, which probably arrived on a ship's hull or oil platform in the late 1980's. The exotic coral was found along a great geographic range of the Canal Central of Ilha Grande, extending over a distance of 25 km. The abundance of Tubastraea was quantified by depth, using three different sampling methods: colony density, visual estimation and intercept points (100 for percentage of cover. Tubastraea showed ample tolerance to temperature and desiccation since it was found more abundantly in very shallow waters (0.1-0.5 m, despite the fact that hard substratum is available at greater depths at all the stations sampled. At most sites, 1 to 5 colonies per 0.25 m² were found most frequently, but occasionally more than 50 colonies were found per 0.25 m², indicating a somewhat gregarious spatial distribution for this coral. The coral Tubastraea was found to occupy slopes of every possible angle in the Canal Central of Ilha Grande, but more colonies were found occupying slopes of 80 to 100°. Therefore, its insensitivity to angles of recruitment and its tolerance for different depths makes it an organism with great ecological tolerance, with a potential to colonize new areas and increase its current range in Brazil's coastal waters.

  11. Spiders from the Island of Fernando de Noronha, Brazil: Part III: Gnaphosidae (Araneae: Arachnida Aranhas da Ilha de Fernando de Noronha, Brasil: Parte III: Gnaphosidae (Araneae: Arachnida

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    Antonio D. Brescovit

    2008-06-01

    Full Text Available The female of Zimiromus hortenciae Buckup & Brescovit, 1993 is described for the first time and Trachyzelotes kulczynskii (Bösemberg, 1902, a species introduced from Europe, is recorded for the first time on the island of Fernando de Noronha, state of Pernambuco. The latter, along with T. lyonneti (Audouim, 1826 recorded from the state of Rio Grande do Sul, is the second species of this genus to be reported from Brazil. The spiders were collected with pitfall traps in five points of the island. Ecological data showed that Gnaphosidae was the fifth best sampled spider family, with 179 specimens, belonging to two species, T. kulczynskii and Z. hortenciae. Trachyzelotes kulczynskii was the most abundant with 118 adults while Z. hortenciae was represented by only 21 adults. Both species were collected during the dry and wet seasons but T. kulczynskii was more abundant during the wet season while Z. hortenciae was more abundant during the dry season. The male:female ratio for the dry season was similar for both species but in the wet season it was three times higher for T. kulczynskii.A fêmea de Zimiromus hortenciae Buckup & Brescovit, 1993 é descrita pela primeira vez. Trachyzelotes kulczynskii (Bösemberg, 1902, uma espécie de origem européia, é registrada pela primeira vez na Ilha de Fernando de Noronha, no estado de Pernambuco. Junto com T. lyonneti (Audouin, 1826, já registrado para o estado do Rio Grande do Sul, Brasil, é a segunda espécie do gênero introduzida no Brasil. As aranhas foram coletadas com armadilhas de solo em cinco pontos da ilha e os dados ecológicos detectados para Gnaphosidae foram de que esta foi a quinta família melhor amostrada dentre Araneae, com 179 espécimes de duas espécies, T. kulczynskii e Z. hortenciae. Trachyzelotes kulczynskii foi mais abundante com 118 adultos enquanto Z. hortenciae teve 21 adultos coletados. Ambas foram coletadas nas estações seca e chuvosa, sendo que T. kulczynskii foi mais

  12. O Conselho Gestor da Área de Proteção Ambiental da ilha do Combu e a experiência da gestão compartilhada.

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    Brenda Batista Cirilo

    2015-11-01

    Full Text Available Este artigo analisa o processo de gestão compartilhada da Área de Proteção Ambiental da Ilha do Combu, unidade de conservação localizada no estuário amazônico, em Belém, Estado do Pará. O objetivo é avaliar seu processo de gestão participativa através da análise da atuação do seu Conselho Gestor. Os resultados foram obtidos através de um levantamento realizado em 2010, com uma amostra de 100 famílias residentes na ilha. A gestão é apresentada no período de 2006 a 2013. Os resultados mostram que: a gestão não funcionou de forma eficaz, pois a maior parte das atividades planejadas não foi concluída; a gestão compartilhada não foi eficiente, apresentando, entre os principais problemas: a ausência e a falta de iniciativa da maioria dos conselheiros membros; a falta de preparo técnico e a descredibilidade nas instituições públicas por parte dos representantes da sociedade civil; o excesso de burocracia e morosidade na resolução de questões administrativas.

  13. O açúcar nas ilhas portuguesas do Atlântico séculos XV e XVI The sugar cane plantation in the Portuguese islands XV and XVI centuries

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    Joaquim Romero Magalhães

    2009-06-01

    Full Text Available A expansão marítima portuguesa teve, em suas possessões nas ilhas do atlântico, um ponto estratégico para a implantação de um sistema de colonização assentado na exploração comercial de determinados bens primários, dentre eles destaca-se o açúcar. Enquanto em algumas ilhas a cana-de-açúcar mostrou-se inviável, em outras foi uma importante fonte de renda, principalmente na exportação do produto para a Europa. A escravidão foi um dos alicerçes essencial do sucesso desse empreendimento, que acabou sendo tranferido para o Brasil, quando essa colônia se mostrou mais vantajosa economicamente.The Portuguese Atlantic islands was during the maritime expansion a strategic point for the deployment of a system of colonization based in the commercial exploration of certain primary goods. The sugar was one of them. While in some islands the sugar cane has proved impracticable, in others it was a source of income, mainly with the exportation of the product to Europe. The slavery was one of the essential foundations for the success of this system. Latter it was transferred to Brazil, where the colonization was more economically advantageous.

  14. Inquérito soro-epidemiológico na Ilha de São Luis durante epidemia de dengue no Maranhão Seroepidemiologic survey in São Luis Island, State of Maranhão, Brazil, during a dengue fever epidemics

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    Pedro Fernando da C. Vasconcelos

    1999-04-01

    Full Text Available Nos anos de 1995 e 1996, ocorreu em São Luis uma epidemia de dengue (DEN, causada pelo sorotipo DEN1. Objetivando avaliar o impacto da mesma na população da grande São Luis (municípios de Paço do Lumiar - PL, São José de Ribamar - SJR e São Luis SL, realizamos um inquérito soro - epidemiológico aleatório, onde aplicamos um questionário. Os soros foram testados por inibição da hemaglutinação (IH, e os resultados, negativo e positivo (resposta primária - RP e resposta secundária - RS, foram analisados utilizando os "software's" Lotus 123, Epi-info 6.0, Excel 5.0 e STATA. Coletaram-se 1217 amostras, (101 de PL, 100 de SJR e 1016 de SL. A positividade foi: 55,4% em PL, 28% em SJR e 41,4% em SL. Destes, 505 (41,2% amostras foram positivas sendo 96 RP (7,9% e 405 RS (33,3%. Da amostra obtida, 508 soros (227 positivos foram do sexo masculino e 709 (278 positivos do feminino, não havendo diferença estatística significativa. Houve significância (p The Island of São Luis in the State of Maranhão, constituted by the municipalities of São Luis - SL (835,428 inhabitants, São José de Ribamar - SJR (60,633 inhabitants and Paço do Lumiar - PL (80,274 inhabitants, has been suffering dengue (DEN fever epidemics since 1995, caused by DEN-1. In 1996, from August through October, an aleatory sero-epidemiologic survey was carried out in order to estimate the incidence of DEN infection and to analyze other clinical and epidemiological parameters. A questionnaire was applied and serum samples were simultaneously obtained. Serum samples were tested by hemagglutination inhibition (HI. Results were analyzed using Lotus123, Epi-info 6.0, Excel 5.0 and STATA softwares. A total of 1,217 serum samples were obtained (101 of PL, 100 of SJR and 1017 of SL. The rate of DEN was 55.4% in PL, 28% in SJR and 41.4% in SL, suggesting the occurrence of 401,933 infections. No difference was seen between males and females, but infection occurred more in the

  15. Tratamento focal e perifocal contra Aëdes aegypti

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    Milton Moura Lima

    1987-06-01

    Full Text Available Em quatro bairros da cidade do Rio de Janeiro, foram feitos ensaios de tratamento focal com abate granulado a 1 ppm e perifocal com pó molhável de Sumition a 2,5%. Esses tratamentos foram feitos tanto isoladamente quanto em conjunto e, também, associados à aplicação de inseticida a ultrabaixo volume. Os índices prediais, levantados um mês depois de terminado o trabalho, mostraram que o tratamento focal dispensa qualquer medida auxiliar. O tratamento perifocal mostrou-se inócuo e incapaz de impedir o aparecimento de larvas de Aëdes aegypti e de outros insetos, em pneus pintados, na face externa, com Sumition e com Malation.In four districts of the city of Rio dc Janeiro focal treatment essays with granulated Abate at 1 ppm and perifocal treatment essays with wettable powder of Sumithion at 2,5% were performed. These were made either alone or in combination as well as associated to insecticides applied at ultra low volume. The premise indices obtained one month after the treatments indicates that the focal treatment alone is effective, no other addicional methods being necessary. The perifocal treatment is not effective and did not prevent the development of Aedes aegypti larvae and other insects in tires which had their external surface painted with Sumithion and Malathion.

  16. Vazio e Espaço. Ilha da Berlenga

    OpenAIRE

    Freire, Carolina Abreu

    2015-01-01

    Durante o último semestre do curso de Arquitectura, no âmbito da disciplina de Projecto X, foi proposta a elaboração de um Refúgio para Barcos e Pessoas no arquipélago das Berlengas. O tema Vazio e Espaçosurge a partir de uma interpretação do conceito de refúgio. Um refúgio, ou um abrigo, é a arquitectura na sua forma mais primitiva, mais rudimentar. A arquitectura, na sua primeira existência, evoluiu da necessidade de criar um espaço de abrigo. Serviu apenas de uma forma f...

  17. Efek Larvasida Bakteri Kitinolitik dari Limbah Kulit Udang terhadap Larva Aedes aegypti

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    Dyah Widiastuti

    2016-06-01

    Full Text Available Abstract. Aedes aegypti is a major vector for Dengue, a deadly disease causing death of millions of people in developing countries both in urban and rural populations. Ae. aegypti control using chemical insecticide was always carried out and lead to a widespread insecticide resistance. Therefore, mosquito biological control is needed to replace the usage of chemical insecticide. A chitinolytic bacteria, was isolated from shrimp’s waste (head and shell. The isolate showed chitinolytic activity as a transparent zone in colony inside the synthetic media, containing (w/v- 0,3 % colloidal chitin, 1% pepton, 0,5% yeast extract, 0,1% NaCl, 0,1% K2HPO4, 0,05% MgSO4.7H2O, 0,001% FeSO4.7H2O, 0,001% ZnSO4.7H2O, and each of 0,0001% CuSO4.5H2O, MnSO4.nH2O and CaCl2.2H2O at pH 7 and 300C after 72 h of incubation. The isolate was identified as gram positive group based on gram staining. In the experimental method, four concentrations of chitinolytic bacteria (4%, 8%, 16% and 32% was exposed to Ae. aegypti larvae. The result showed that chitinolytic bacterium degrades exoskeleton of third instar larvae of Ae. aegypti. Degradation of exoskeleton started on the 2nd days and occurred in thorax region. Probit analysis showed LC50 value was obtaninedat concentration of 2%. Keywords: chitinolytic bacteria, shrimp’s waste, Aedes aegypti Abstrak. Aedes aegypti merupakan vektor utama Dengue, penyakit yang menyebabkan kematian jutaan orang di negara-negara berkembang baik pada populasi perkotaan dan pedesaan. Pengendalian Ae. aegypti menggunakan insektisida kimia selalu dilakukan dan menyebabkan resistensi insektisida secara luas. Oleh karena itu, pengendalian nyamuk secara biologis diperlukan untuk menggantikan penggunaan insektisida kimia. Bakteri kitinolitik telah diisolasi dari limbah udang (kepala dan cangkang. Isolat menunjukkan aktivitas kitinolitik berupa zona bening di sekitar  koloni dalam media sintetik yang mengandung (w/v - 0,3% koloidal kitin, 1

  18. Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus.

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    Thais Chouin-Carneiro

    2016-03-01

    Full Text Available Since the major outbreak in 2007 in the Yap Island, Zika virus (ZIKV causing dengue-like syndromes has affected multiple islands of the South Pacific region. In May 2015, the virus was detected in Brazil and then spread through South and Central America. In December 2015, ZIKV was detected in French Guiana and Martinique. The aim of the study was to evaluate the vector competence of the mosquito spp. Aedes aegypti and Aedes albopictus from the Caribbean (Martinique, Guadeloupe, North America (southern United States, South America (Brazil, French Guiana for the currently circulating Asian genotype of ZIKV isolated from a patient in April 2014 in New Caledonia.Mosquitoes were orally exposed to an Asian genotype of ZIKV (NC-2014-5132. Upon exposure, engorged mosquitoes were maintained at 28° ± 1 °C, a 16h:8h light:dark cycle and 80% humidity. 25-30 mosquitoes were processed at 4, 7 and 14 days post-infection (dpi. Mosquito bodies (thorax and abdomen, heads and saliva were analyzed to measure infection, dissemination and transmission, respectively. High infection but lower disseminated infection and transmission rates were observed for both Ae. aegypti and Ae. albopictus. Ae. aegypti populations from Guadeloupe and French Guiana exhibited a higher dissemination of ZIKV than the other Ae. aegypti populations examined. Transmission of ZIKV was observed in both mosquito species at 14 dpi but at a low level.This study suggests that although susceptible to infection, Ae. aegypti and Ae. albopictus were unexpectedly low competent vectors for ZIKV. This may suggest that other factors such as the large naïve population for ZIKV and the high densities of human-biting mosquitoes contribute to the rapid spread of ZIKV during the current outbreak.

  19. Evaluation of toxic effects with transition metal ions, EDTA, SBTI and acrylic polymers on Aedes aegypti (L., 1762 (Culicidae and Artemia salina (Artemidae

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    Eduardo José de Arruda

    2011-06-01

    Full Text Available This work aimed to evaluate the toxicity of some insecticides compounds on Aedes aegypti and Artemia salina larvae. Bioassays were carried out to evaluate the toxic effect after of 24 and 72 h using the compounds or associations. The LC10, LC50 and LC90 values were obtained and utilized for toxicity comparations. For Ae. aegypti, LC50 were 32.65 mg L-1 in 24 h for Na2[EDTA-Cu(II] and total mortality in 72 h for SAP-Na2[EDTA-Cu(II].

  20. Parque Estadual da Ilha Anchieta – levantamento da qualidade da experiência do visitante. Ilha Anchieta State Park – visitor experience quality assessment.

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    Silvia Yochie KATAOKA

    2011-06-01

    Full Text Available A experiência de visitantes em uma área natural protegida pode ocorrer de forma diversificada entre diferentes públicos, associada à própriamaneira de avaliar a experiência e às expectativas iniciais. Fatores culturais, contexto socioeconômico e o grau de familiaridade dos diferentes indivíduos com o meio natural são determinantes à maneira com a qual eles recebem e percebem os benefícios de seus contatos com uma Unidade de Conservação. O Parque Estadual da Ilha Anchieta – PEIA foi eleito como área deste estudo por reunir a qualidade de praia e de ilha, dois fatores associados a expectativas relativamente diferentes, uma vez que ilhas sugerem sensação de isolamento e distância do cotidiano e praias estão maisassociadas ao convívio social e atividades recreativas. Com o objetivo de compreender as relações entre o visitante e as áreasnaturais e fornecer ferramentas ao manejo do uso público no Parque em questão, foram feitas entrevistas com visitantes durante a alta temporada de visitação do ano de 2004. Os resultados referentes às motivações e avaliações de aspectos de suas visitas revelam a existência de dois principais grupos de frequentadores que apresentam visões e comportamentos conflitantes. Entretanto, ao mesmo tempo em que o Parque recebe visitantes tão diversificados, a apreciação do contato com o ambiente natural se mostrou como um elo entre ambos, viabilizando ações de manejo do uso público que amplifiquem a qualidade da experiência de todos os visitantes com o ambiente natural.A Visitor experience in a natural protected area may occur differently among a different public, being associated to the way they evaluate their own experience and their first expectations. Cultural factors, social economic context and familiarity degree of the natural environment are crucial for how individuals receive and perceive the benefits of their contacts to a Protected Area. The State Park of Ilha Anchieta

  1. Aedes aegypti: modelo experimental de atividade biológica de fitoprodutos

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    Michele Teixeira Serdeiro

    2017-06-01

    Full Text Available Aedes aegypti (Linnaeus, 1762 é reconhecido como transmissor de várias arboviroses de importância na saúde pública, como a dengue, zika, chikungunya e febre amarela urbana. O principal método de prevenir a transmissão desses vírus ainda é o controle do mosquito vetor. Produtos naturais de origem vegetal vêm sendo investigados, como mais uma ferramenta no controle de vetores, e compostos menos impactantes ao meio ambiente e a saúde humana. Devido à importância deste culicídeo, buscou-se extrato e frações de C. catharinensis com atividade larvicida sobre Ae. aegypti. O extrato bruto metanólico (EBM e sua fração (EBM 1 obtidos da embaúba foram aplicados no meio de criação das larvas (L3 nas concentrações de 10, 30 e 50 μg/mL. O tratamento com C. cahtarinensis resultou na alteração do período de desenvolvimento larval, pupal e de L3-adulto do mosquito. A mortalidade pupal (25% foi obtida pela fração EBM1. Este estudo demonstrou a eficácia de C. catharinensis sobre o período de desenvolvimento de Ae. aegypti.

  2. First records of Freshwater Bivalves of Ilha Grande National Park, Paraná, Brazil

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    Flávio Henrique Ragonha

    2014-03-01

    Full Text Available The Ilha Grande National Park, Paraná, Brazil, is located in the Upper Paraná River and has characteristics typical of a floodplains. This protected area includes lagoons connected and disconnected to the Paraná River, although the latter also connect during periods of high water level, thus composing a heterogeneous group of lacustrine environments. The enormous potential the flora and fauna diversities are still little known to the region, as can be seen through benthic invertebrates, inclunding bivalves mollusks. The granulometric composition of these floodplain lagoons was formed mainly by mud and very fine sand. Furthermore, organic matter composition was predominantly of fine particulate. The other abiotic factors differed from lagoons located within the island of the park to those located in the left margin of Paraná River. The results demonstrated the importance of abiotic factors such as the physical composition of granulometric texture, organic matter and macrophyte banks, to the establishment of bivalves in these floodplain lagoons. We recorded bivalves of Pisidium (native, Diplodon (native, and Corbicula (invasive. The highest values of Diplodon sp. density were observed at São João/C lake, for Pisidium sterkianum (Pilsbry, 1897 at São João/M lake, and to Jatobá/C lagoon with high density of invasive species Corbicula fluminea (Müller, 1774. This study to obtain conduct the first records of freshwater bivalves in floodplains lagoon in the Ilha Grande National Park, and provides contributions to better understanding the ecology of these mollusks. The recording of native species in the region of Upper Paraná River floodplain after a lomg period without new records, demonstrated the importance of protecting the lagoons of the Ilha Grande National Park as they can be a possible refuge to some species of native freshwater bivalves.

  3. The Macuti House, Traditional Building Techniques and Sustainable Development in Ilha de Moçambique

    DEFF Research Database (Denmark)

    Sollien, Silje Erøy

    2011-01-01

    This paper is part of the initial phase of PhD research focusing on conservation of the macuti architecture in the World Heritage City of Ilha de Moçambique. It questions how initiatives to preserve traditional ways of building in this area, of which parts could be described as an urban slum, may...... subordination, poverty and low social status, such initiatives need to be part of a wider programme of strengthening cultural and social capital among the population, avoiding division into tangible and intangible heritage management, and include broad ecological and socio-economic considerations....

  4. Panstrongylus megistus em ecótopos artificiais de ilhas do Alto Rio Paraná

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    Guilherme Ana Lucia Falavigna

    2001-01-01

    Full Text Available Em resposta a denúncias de triatomíneos em ilhas do Alto Rio Paraná foram investigados 145 ecótopos artificiais e 4 (2,8% deles encontravam-se infestados: residência, "clube", ex-escola e monte de madeira. Foram analisados 17 de 35 P. megistus coletados; 12 (70,6% apresentavam-se infectados por Trypanosoma cruzi. Ave e roedor constituíram as fontes alimentares mais comuns. Todos os exames sorológicos (56 de humanos, 18 de cães e 10 de gatos foram negativos.

  5. Leishmaniasis transmission in an ecotourism area: potential vectors in Ilha Grande, Rio de Janeiro State, Brazil.

    Science.gov (United States)

    Carvalho, Bruno Moreira; Maximo, Michele; Costa, Wagner Alexandre; de Santana, Antonio Luís Ferreira; da Costa, Simone Miranda; da Costa Rego, Taiana Amancio Neves; de Pita Pereira, Daniela; Rangel, Elizabeth Ferreira

    2013-11-13

    The south coast of Rio de Janeiro State, in Brazil, is endemic for cutaneous and visceral leishmaniases and is frequently visited by tourists from different parts of the world. Since the complex epidemiology of leishmaniases demands local studies, the goal of this study was to investigate the phlebotomine sand fly fauna and leishmaniases transmission in Ilha Grande, an ecotourism area of Angra dos Reis municipality. Sand fly fauna was sampled in three monitoring stations using HP light traps in domiciles, peridomiciles and forests. Species abundance was evaluated by the Index of Species Abundance. A Leishmania natural infection survey was done using multiplex PCR and dot blot hybridization. During 15 consecutive months of sand fly monitoring, 1093 specimens from 16 species were captured. The potential leishmaniases vectors found were Lutzomyia (Nyssomyia) intermedia, L. migonei, L. (N.) flaviscutellata, L. (Psychodopygus) ayrozai and L. (Lutzomyia) longipalpis. Five species were new records in Ilha Grande: L. (Sciopemyia) microps, L. termitophila, L. firmatoi, L. rupicola and L. (P.) ayrozai. Higher species richness was found inside forest areas, although potential leishmaniases vectors were present in deforested areas, peridomiciles and inside houses. Lutzomyia (N.) intermedia and L. migonei were the most abundant species. Females of L. migonei showed a high rate (10.3%) of natural infection by Leishmania (Viannia) sp., probably Leishmania (V.) braziliensis. The detection of leishmaniases transmission and potential vectors in Ilha Grande is of public health concern, especially because tourists are frequently visiting the island. Besides reinforcing the epidemiological importance of L. (N.) intermedia in Rio de Janeiro State, the role of L. migonei in cutaneous leishmaniasis transmission is highlighted with its high rate of Leishmania natural infection. The finding of L. (L.) longipalpis confirmed the human autochthonous case of visceral leishmaniasis from the

  6. Um caso de leucismo em pardal, Passer domesticus (Linnaeus, 1758 em uma ilha do rio São Francisco, nordeste do Brasil

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    Leonardo Barros Ribeiro

    2011-10-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n1p187 O leucismo em aves é uma anomalia genética caracterizada pela ausência total de melanina em algumas ou em todas as penas, porém, ao contrário do albinismo, as outras partes do corpo, tais como olhos, bico e tarsos permanecem com a coloração típica da espécie. O pardal Passer domesticus é uma ave nativa da Europa, Ásia e norte da África e foi introduzido na América do Norte, América do Sul, África do Sul, Austrália e Nova Zelândia. Atualmente, é a espécie de ave com a maior distribuição geográfica. Relatamos, aqui, o registro de um indivíduo leucístico de Passer domesticus na ilha do Rodeadouro, rio São Francisco, nordeste do Brasil.

  7. Determination of circulation and short period fluctuation in Ilha Grande Bay (RJ, Brazil

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    Yoshimine Ikeda

    1980-06-01

    Full Text Available A mesosoale study was made of the Ilha Grande area. The local circulation described through -progressive vector diagrams showed a clochwise bottom circulation determined in June 1976, while in the upper 10 m the direction of the flow entering the Ilha Grande Bay was towards the center in the west and towards the Marambaia sandbank in the east side of the Bay. Short periods and amplitude fluctuations were evaluted using power spectral analysis, Fourier and Maximum Entropy Method, which showed that in the upper 10 m predominant periods decrease from 1.1h (A = 6.3cm sec-1 (position = 3C to 1.0h (A = 7.4 cm sec-1 (position = 2D and increase to 5.8h (A = 6.8 cm., sec-1 (position = ID, while at the bottom layer the predominant period increases from 0.4 h (A = 5.0 cm sec-1 (position = 3G to 6.4h (A = 7.0 cm sec-1 (position = 2G and to 4.4h (A = 7.9 cm sec-1 (position = 1G . From the original data it has been possible to determine an "intense pulsation" between 30-70 cm sec-1 in the upper 10 m with about 1.0h period and 10-20 min duration in all the stations.

  8. Insect galls of restinga areas of Ilha da Marambaia, Rio de Janeiro, Brazil

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    Alene Ramos Rodrigues

    2014-06-01

    Full Text Available Insect galls of restinga areas of Ilha da Marambaia, Rio de Janeiro, Brazil. This study carried out an insect gall inventory in restinga areas of Ilha da Marambaia, in the municipality of Mangaratiba, Rio de Janeiro, Brazil. Sampling was carried out monthly from April 2010 to March 2011 along the full extension of seven beaches. A total number of 147 gall morphotypes associated with 70 plant species were found, distributed in 33 plant families, and at least 54 genera. Myrtaceae was the botanical family with the highest richness of gall morphotypes and host species, followed by Bignoniaceae, Fabaceae, Asteraceae, Euphorbiaceae, Sapindaceae, and Malpighiaceae. Most of the gall morphotypes occurred in leaves (78 morphotypes, 38 in stems, 14 in flowers, eight in buds and fruits, and one in adventitious roots. The galling insects belong to the five orders: Diptera, Coleoptera, Hemiptera, Lepidoptera, and Thysanoptera. Cecidomyiidae (Diptera was the most common galling taxon (78 morphotypes, represented by 87 species, being 78 gallers, seven inquilines and two predators. In addition to the gallers, parasitoids, inquilines, and predators were also found.

  9. Water percolation conditions in Ilha Solteira dam (Parana River), using tracer techniques

    International Nuclear Information System (INIS)

    Sanchez, W.; Guidicini, G.; Silva, R.F. da.

    1975-01-01

    Radioisotopic techniques used in the study of water perconlation at the exact place of the construction of the canal lock of Ilha Solteira Dam, in its left side is presented. At the time of the drilling operations, it was discovered, by water leakage tests, total lost at 275,00 level. This water lost occurred at the vicinities of basalt lava-flows. The water leakage tests showed that the total absorption of pumping flow was about 80 liters per minute. To determine the velocity of water percolation in the probable cracks or fractures of the basalt a test of radioactive tracer 131 I was used. For the study of the radioactive tracer behaviour two techniques were tried: measurement of its dilution in the original of the well and the measurement of residence time. Results from the tests showed the existence of a crack or a set of cracks oriented from the radcoisotopic injection well to the artesian wells located at the left shore of Parana river, below Ilha Solteira Dam. The mentioned cracks are localized at the 272,00 level, close tr the contact between the basalt lava-flows

  10. Reproductive Dynamics of Sterna hirundinacea Lesson, 1831 in Ilha dos Cardos, Santa Catarina, Brazil

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    Hélio Augusto Alves Fracasso

    2014-01-01

    Full Text Available In this work, we intend to describe the reproductive dynamics of Sterna hirundinacea in an island from South Brazil. We studied the reproductive biology of this species in its natural environment and provide data on their growth, survival, and reproductive success in Ilha dos Cardos, Santa Catarina, South Brazil. Samplings were carried out daily on the island throughout the reproductive seasons of 2003, 2005, and 2006 and the different stages of development of the chicks were characterized according to age, length of the beak, and plumage characteristics. We provide a basic equation Lm=167.91 (1-e-0.062t--0.23 to determine the approximate age of individuals using their body mass. The main cause of chick mortality on the island was natural (63.17% in 2003, 81.41% in 2005, and 79.96% in 2006, whereas predation contributed to mortality in a proportion of 38.83% in 2003, 18.59% in 2005, and 20.04% in 2006. The absence in the area of the chicks’ main predator, Kelp gull (Larus dominicanus, the large number of chicks that reached the final stages of development, and their reproductive success demonstrate that Ilha dos Cardos is an important breeding site for the species in southern Brazil.

  11. Do Convento de Jesus, na Ribeira Grande (S. Miguel), no século XVII : as cartas de dote para freira

    OpenAIRE

    Gonçalves, Maria Margarida de Sá Nogueira Lalanda

    1995-01-01

    "Em 1555 a vila da Ribeira Grande é contemplada com a fundação do terceiro mosteiro de Clarissas erigido na ilha de S. Miguel: trata-se do Convento de Jesus, que se segue cronologicamente ao de Santo André, em Vila Franca do Campo (existente desde 1533, quando a sua primitiva comunidade se transferiu da casa edificada sobre as rochas costeiras de Vale de Cabaços, hoje Caloura, para a primeira «capital» da ilha) e ao de Nossa Senhora da Esperança, em Ponta Delgada (criado em 1541). [...]"

  12. CARACTERIZAÇÃO E AVALIAÇÃO DA ATIVIDADE LARVICIDA DO ÓLEO ESSENCIAL DO Zingiber officinale Roscoe (GENGIBRE) FRENTE AO MOSQUITO Aedes aegypti

    OpenAIRE

    ANDRESON LEANDRO SANTANA SILVA

    2012-01-01

    Na procura pelo controle químico alternativo contra o mosquito Aedes aegypti, diversas pesquisas são desenvolvidas e estimuladas no intuito de descobrirem novas substâncias inseticidas de origem vegetal. Neste trabalho a partir da extração e do estudo analítico do óleo essencial dos rizomas do Zingiber officinale Roscoe, foi analisado o efeito larvicida do óleo contra larvas em terceiro estágio do mosquito Aedes aegypti (Linnaeus, 1792). Extraiu-se quantitativamente o óleo essencial por hidro...

  13. [Aedes aegypti control strategies: a review].

    Science.gov (United States)

    Zara, Ana Laura de Sene Amâncio; Santos, Sandra Maria Dos; Fernandes-Oliveira, Ellen Synthia; Carvalho, Roberta Gomes; Coelho, Giovanini Evelim

    2016-01-01

    to describe the main strategies to control Aedes aegypti, with emphasis on promising technological innovations for use in Brazil. this study is a non-systematic review of the literature. several technologies have been developed as alternatives in the control of Ae. aegypti, using different mechanisms of action, such as selective monitoring of the infestation, social interventions, dispersing insecticides, new biological control agents and molecular techniques for population control of mosquitoes, also considering the combination between them. Evolving technologies require evaluation of the effectiveness, feasibility and costs of implementation strategies as complementary to the actions already recommended by the National Program for Dengue Control. the integration of different compatible and effective vector control strategies, considering the available technologies and regional characteristics, appears to be a viable method to try to reduce the infestation of mosquitoes and the incidence of arbovirus transmitted by them.

  14. Recomendaciones para la vigilancia de Aedes aegypti

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    Roberto Barrera

    2016-09-01

    Full Text Available La enfermedades causadas por arbovirus transmitidos por Aedes aegypti, como el dengue, el chikungunya y el zika, continúan aumentando en incidencia anual y expansión geográfica. Una limitación clave para el control de A. aegypti ha sido la ausencia de herramientas eficaces para vigilar su población y poder determinar las medidas de control que realmente funcionan. La vigilancia de A. aegypti se ha basado principalmente en la obtención de los índices aédicos, los cuales guardan poca relación con el número de hembras del mosquito, que son las responsables de la transmisión de los virus. El reciente desarrollo de técnicas de muestreo de adultos de este vector promete facilitar las labores de vigilancia y control. En esta revisión se presentan las diversas técnicas de vigilancia del mosquito, así como una discusión sobre su utilidad, con recomendaciones para lograr una vigilancia entomológica más efectiva.

  15. ANÁLISE DO PROCESSO DE USO E OCUPAÇÃO DA ORLA DA PRAIA DO AREIÃO (ILHA DE MOSQUEIRO-PA, TENDO EM VISTA O CUPRIMENTO DAS DIRETRIZES DO PLANO NACIONAL DE GERENCIAMENTO COSTEIRO (LEI 7.661/88./ Analysis of using and occupation process of Areião Beach Edge

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    Ivan Gomes Viana

    2017-12-01

    Full Text Available Ao longo do processo de integração da ilha de Mosqueiro à região metropolitana de Belém, grande parte da população concentrou-se na zona costeira. Essa atração populacional se deu, sobretudo, devido às potencialidades turísticas da ilha. Segundo o Plano Nacional de Gerenciamento Costeiro, por estar localizada em um estuário, a orla de Mosqueiro está dentro da área de abrangência da zona costeira brasileira, bem como, é amparada por uma legislação específica para estes espaços. Na extensão da praia do Areião são identificadas distintas formas de uso e ocupação que interagem com a dinâmica natural da praia, podendo ser caracterizados 4 setores na praia. Estes setores apresentam particularidades com relação ao cumprimento de algumas diretrizes estabelecidas pela legislação que ordena o uso e a ocupação da zona costeira.

  16. Exile Island and Global Conversation: Ilha do Desterro Bridges Languages and Cultures

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    Renata Wasserman

    2016-06-01

    Full Text Available http://dx.doi.org/10.5007/2175-8026.2016v69n2p207 This is a concise overview of the publication history of Ilha do Desterro, which shows some changes in format, but a consistent and ever-widening interest in language broadly deined, from linguistics to literature to ilm, as it manifests itself in diferent languages, places, and times. he journal publishes in English and Portuguese, but this overview, aware of the impossibility of covering the entire array of essays that appeared in its extended history, limits itself to notes on articles dealing with Anglophone expression by itself and in comparison to its counterparts in the Lusophone world.

  17. Toksisitas Insektisida Organofosfat Dan Karbamat Terhadap Nyamuk Aedes aegypti

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    Endang Puji Astuti

    2013-02-01

    Full Text Available Abstract. Aedes aegypti mosquito is increasing problem of public health, being the vector responsible for Dengue and Chikungunya. Chlorpirifos (Organofosfat and Metonil (Carbamate were known to posses insecticide activity against insect. The study was aimed to examine effectiveness of Chlorpirifos and Metonil as insectiside against Ae. aegypti mos­quito Chlorpirifos a significantly higher insecticide activity against Ae. aegypti_than Metonil. The mosquito mortality was observed after 24 h exposure. The LCso value of Chlor­pirifos and Metonil were 0.64 mg/lt and 0,802 mg/lt, against Ae. aegypti mosquito. The mixed of both insecticide was LCso value 108.04 mg/lt, this result prove that mixed of both insecticede not sinergism. The result of th is study suggested that Chlorpirifos more effective insecticide against Ae. aegypti than Metonil. Key Words : Culex quinquefasciatus, insecticide, chlorpirifos, metonil

  18. Gentrificação nas Ilhas do Porto: uma dinâmica embrionária

    OpenAIRE

    Matos, Fátima Loureiro de

    2017-01-01

    Neste artigo pretende-se evidenciar dinâmicas que estão a ocorrer nas ilhas da cidade do Porto, associadas à expansão da atratividade turística, que tem desencadeado a reabilitação e a gentrificação de alguns contextos urbanos. Trata-se de um trabalho exploratório, ainda em curso, baseado em trabalho de campo, algumas entrevistas e análises estatísticas. As ilhas são um património histórico e da vida da cidade, que pela sua morfologia urbana específica, atrai novos residentes e turistas....

  19. A renúncia de Fidel Castro: continuidade na sucessão presidencial

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    Isabele Villwock Bachtold

    2010-10-01

    Full Text Available Cuba atualmente vive uma nova fase, pautada na possível reforma do sistema socialista em vigor no país desde a Revolução Cubana de 1959. Este artigo busca analisar as implicações que a recente renúncia de Fidel Castro à presidência cubana acarretará para a ilha e para o cenário internacional como um todo.

  20. Screening of plants found in the State of Amazonas, Brazil for activity against Aedes aegypti larvae Triagem de plantas encontradas no Estado do Amazonas para atividade larvicida contra Aedes aegypti

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    Adrian Martin Pohlit

    2004-01-01

    Full Text Available Ethanol, methanol and water extracts representing mostly native plant species found in the Amazon region were prepared, respectively, by maceration, continuous liquid-solid extraction and infusion, followed by evaporation and freeze-drying. The freeze-dried extracts were tested for lethality toward Aedes aegypti larvae at test concentrations of 500 mg / mL. In general, methanol extracts exhibited the greatest larvicidal activity. The following 7 methanol extracts of (the parts of the indicated plant species were the most active, resulting in 100% mortality in A. aegypti larvae: Tapura amazonica Poepp. (root, Piper aduncum L. (leaf and root, P. tuberculatum Jacq. (leaf, fruit and branch. and Simaba polyphylla (Cavalcante W.W. Thomas (branch.Extratos aquosos, etanólicos e metanólicos, representando principalmente espécies vegetais nativas encontradas na região Amazônica, foram preparados, respectivamente, por infusão, maceração e extração contínua líquido-sólido, seguida de evaporação e liofilização. Os extratos liofilizados foram testados para atividade contra larvas de Aedes aegypti, na concentração única de 500 mg / mL. Os extratos metanólicos foram, em geral, os que apresentaram maior atividade larvicida. Os seguintes 7 extratos metanólicos das (partes das espécies vegetais indicadas foram os mais ativos, provocando 100% de mortalidade em larvas de A. aegypti: Tapura amazonica Poepp. (raiz, Piper aduncum L. (folha e raiz, P. tuberculatum Jacq. (folha, fruto e galho e Simaba polyphylla (Cavalcante W.W. Thomas (galho.

  1. PENGENDALIAN JENTIK Aedes Aegypti MENGGUNAKAN Mesocyclops Aspericomis MELALUI PARTISIPASI MASYARAKAT

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    Umi Widyastuti

    2012-07-01

    Full Text Available Mesocyclops aspericornis was investigated for its effectiveness in controlling Aedes aegypti larvae in a variety of containers e.g metal drum, cistern, clay jars, and other container made of plastic. A study was carried out in Kenteng hamlet, Tegalrejo village, Salatiga Municipality. It was conducted by health-workers (staff of Vector and Reservoir Control Research Unit and Health Center of Tegalrejo and the community, especially the woman's organization namely "family empowering and welfareness ". which participate in releasing M. aspericornis for controlling Ae. aegypti larvae. The community has responsibility to release M. aspericornis in Kenteng RT01 and 02 as the treated area I. Meanwhile, Health-workers have responsibility to release it in Kenteng RT 04, 05, and 07 as the treated area II and Kenteng RT 03 and 06 as the untreated control area (no M. aspericornis released. The aim of the study were: a, to determine the effectiveness of M. aspericornis in decreasing larval populations of Ae. aegypti in the containers, and b. to determine the Knowledge, Attitude, and Practice (KAP of the community, referring to disease, vector and control of Dengue Haemorhagic Fever (DHF. M. aspericornis was effective to decrease larval populations of Ae. aegypti in Kenteng area. The increasing number of Ae. aegypti larvae free containers of 24.29-84,02% and 35.75-92.01% were shown in respectively treated area I and II. The KAP of the community referring to disease, vector and control of DHF increased after the health education conducted. It's concluded that the community of Kenteng hamlet is active in participation to control Ae. aegypti. As a recommend, control of Ae. aegypti larvae using M. aspericornis through community partisipation should be considered due to a good prospect and effectiveness of this agent to control of Ae. aegypti larvae in the laboratory as well as in the field   Key words : Vector control, M. aspericornis, Ae. aegypti, Dengue

  2. Ácaros de penas e carrapatos (Acari associados a Turdus albicollis Vieillot (Aves, Muscicapidae em uma área de Mata Atlântica da Ilha Grande, Rio de Janeiro, Brasil Feather mites and ticks (Acari associated to Turdus albicollis Vieillot (Aves, Muscicapidae in an area of Atlantic Forest at Ilha Grande, Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Alline Storni

    2005-06-01

    Full Text Available O parasitismo é um importante mecanismo que afeta populações e comunidades. O objetivo deste estudo foi investigar a fauna de ectoparasitos que habita o corpo do sabiá-de-coleira, Turdus albicollis Vieillot, 1818 e avaliar se a massa corporal do hospedeiro é afetada por estes parasitos. Os indivíduos de T. albicollis foram mensalmente capturados na Ilha Grande, Rio de Janeiro, no período de julho de 1999 a junho de 2000, em uma área de Floresta Atlântica. As aves foram individualmente marcadas, pesadas e examinadas para registrar e quantificar a presença de ectoparasitos. A abundância e a localização dos parasitos no corpo do hospedeiro foram registradas. Em 54 indivíduos de T. albicollis amostrados, foram encontrados duas espécies de ectoparasitos. A prevalência de ácaros de penas, Pterodectes turdinus Berla, 1959, foi de 72,2% enquanto que a de carrapatos, Amblyomma longirostre Koch, 1844, foi de 27,8%. A abundância mensal de P. turdinus foi significativamente relacionada com os meses do ano, sendo maior nos meses com menor freqüência de chuva. Não houve relação estatisticamente significativa entre a massa corporal do hospedeiro (g e a abundância total de ácaros de penas e carrapatos.Parasitism is an important mechanism affecting populations and communities. The purpose of this study was to investigate the ectoparasites fauna living on the body of the white-necked trush, Turdus albicollis Vieillot, 1818, and to evaluate if the host body mass is affected by these parasites. Turdus albicollis were monthly captured at Ilha Grande, Rio de Janeiro, from July 1999 to June 2000 in an area of Atlantic Forest. The birds were individualy marked, weighed and carefully checked to record and quantify the presence of ectoparasites. Parasite abundance and location on the bird's body were recorded. In 54 individuals of T. albicollis sampled, two ectoparasite species were found. The prevalence of the feather mite Pterodectes turdinus

  3. The immune strategies of mosquito Aedes aegypti against microbial infection.

    Science.gov (United States)

    Wang, Yan-Hong; Chang, Meng-Meng; Wang, Xue-Li; Zheng, Ai-Hua; Zou, Zhen

    2018-06-01

    Yellow fever mosquito Aedes aegypti transmits many devastating arthropod-borne viruses (arboviruses), such as dengue virus, yellow fever virus, Chikungunya virus, and Zika virus, which cause great concern to human health. Mosquito control is an effective method to block the spread of infectious diseases. Ae. aegypti uses its innate immune system to fight against arboviruses, parasites, and fungi. In this review, we briefly summarize the recent findings in the immune response of Ae. aegypti against arboviral and entomopathogenic infections. This review enriches our understanding of the mosquito immune system and provides evidence to support the development of novel mosquito control strategies. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Fauna de mosquitos asociada con Aedes aegypti en Guaduas, Colombia

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    Víctor Alberto Olano

    1993-06-01

    Full Text Available Durante un estudio sobre la ecología del Aedes aegypti llevado a cabo en el área urbana de Guaduas, Colombia, se hallaron un total de siete especies de mosquitos que compartían hábitats con esta especie. Los criaderos en los cuales se encontró un mayor número de mosquitos asociados con el Aedes aegypti fueron los tanques bajos (albercas. Larvas de Toxorhynchites spp. se encontraron en tanques elevados. Se discute la importancia de estos hallazgos con relación a los aspectos de ecología y control del Aedes aegypti.

  5. Algas marinhas bentônicas da Reserva Biológica Estadual da Praia do Sul, Ilha Grande, Angra dos Reis, Rio de Janeiro, Brasil Benthic marine algae of the State Biological Reserve of Praia do Sul, Ilha Grande, Angra dos Reis, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Lísia Mônica de Souza Gestinari

    1998-04-01

    Full Text Available Na Reserva foram encontradas 121 espécies, sendo 27 de Chlorophyceae, 21 de Phaeophyceae e 73 de Rhodophyceae. Dentre estas espécies, 38 são novas referências para a ilha, sendo que Cladophora catenata (L. Kütz. é pela primeira vez citada para o litoral do Rio de Janeiro. Não foram encontradas espécies endêmicas e nem flora específica de região insular. A comparação da flora da Reserva com a de regiões vizinhas indica alta percentagem de espécies em comum entre elas.A survey in the Reserve showed 27 species of Chlorophyceae, 21 of Phaeophyceae and 73 of Rhodophyceae. Among the recorded species, 38 are new references to the Island and Cladophora catenata (L. Kütz. is for the first time quoted to the Rio de Janeiro coast. Neither endemic species nor specific flora from insular area were found in the Reserve. A comparison between the flora of the Reserve and those of neighboring areas, showed a high percentage of similarity.

  6. The impact of insecticide applications on the dynamics of resistance: The case of four Aedes aegypti populations from different Brazilian regions

    Science.gov (United States)

    Martins, Ademir de Jesus; Maciel-de-Freitas, Rafael; Linss, Jutta Gerlinde Birggitt; Araújo, Simone Costa; Lima, José Bento Pereira; Valle, Denise

    2018-01-01

    Background In the tropics, the utilization of insecticides is still an important strategy for controlling Aedes aegypti, the principle vector of dengue, chikungunya and Zika viruses. However, increasing insecticide resistance in Ae. aegypti populations might hinder insecticide efficacy on a long-term basis. It will be important to understand the dynamics and evolution of insecticide resistance by assessing its frequency and the mechanisms by which it occurs. Methodology/Principal findings The insecticide resistance status of four Brazilian Ae. aegypti populations was monitored. Quantitative bioassays with the major insecticides employed in the country was performed: the adulticide deltamethrin (a pyrethroid—PY) and the larvicides, temephos (an organophosphate) and diflubenzuron (a chitin synthesis inhibitor). Temephos resistance was detected in all populations although exhibiting a slight decrease over time probably due to the interruption of field use. All vector populations were susceptible to diflubenzuron, recently introduced in the country to control Ae. aegypti. Resistance against deltamethrin was extremely high in three populations. Molecular assays investigated substitutions in the voltage gated sodium channel (NaV), the PY target site, at positions 1011, 1016 and 1534. Elevated frequencies of substitutions Val1016Ile and Phe1534Cys related to high PY resistance levels were identified. Biochemical assays detected alterations in the activities of two detoxifying enzyme classes related to metabolic resistance, glutathion-S-transferases and esterases. The results obtained were evaluated in the context of both recent insecticide use and the records of dengue incidence in each locality. Conclusions/Significance The four Ae. aegypti populations evaluated were resistant to the neurotoxic insecticides, temephos and deltamethrin. However, they were still susceptible to diflubenzuron. A probable correlation between adult insect resistance to PY and the domestic

  7. Dengue virus transovarial transmission by Aedes aegypti

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    Monica Dwi Hartanti

    2016-02-01

    Full Text Available Dengue is a disease that is caused by dengue virus and transmitted to humans through the bite of infected Aedes mosquitoes, especially Aedes aegypti. The disease is hyper-endemic in Southeast Asia, where a more severe form, dengue hemorrhagic fever (DHF and dengue shock syndrome (DSS, is a major public health concern. The purpose of the present study was to find evidence of dengue virus transovarial transmision in local vectors in Jakarta. Fifteen Aedes larvae were collected in 2009 from two areas in Tebet subdistrict in South Jakarta, namely one area with the highest and one with the lowest DHF prevalence. All mosquitoes were reared inside two cages in the laboratory, eight mosquitoes in one cage and seven mosquitoes in another cage and given only sucrose solution as their food. The results showed that 20% of the mosquitoes were positive for dengue virus. Dengue virus detection with an immunohistochemical method demonstrated the occurrence of transovarial transmission in local DHF vectors in Tebet subdistrict. Transovarial dengue infection in Ae.aegypti larvae appeared to maintain or enhance epidemics. Further research is needed to investigate the relation of dengue virus transovarial transmission with DHF endemicity in Jakarta.

  8. Dengue virus transovarial transmission by Aedes aegypti

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    Monica Dwi Hartanti

    2010-08-01

    Full Text Available Dengue is a disease that is caused by dengue virus and transmitted to humans through the bite of infected Aedes mosquitoes, especially Aedes aegypti. The disease is hyper-endemic in Southeast Asia, where a more severe form, dengue hemorrhagic fever (DHF and dengue shock syndrome (DSS, is a major public health concern. The purpose of the present study was to find evidence of dengue virus transovarial transmision in local vectors in Jakarta. Fifteen Aedes larvae were collected in 2009 from two areas in Tebet subdistrict in South Jakarta, namely one area with the highest and one with the lowest DHF prevalence. All mosquitoes were reared inside two cages in the laboratory, eight mosquitoes in one cage and seven mosquitoes in another cage and given only sucrose solution as their food. The results showed that 20% of the mosquitoes were positive for dengue virus. Dengue virus detection with an immunohistochemical method demonstrated the occurrence of transovarial transmission in local DHF vectors in Tebet subdistrict. Transovarial dengue infection in Ae.aegypti larvae appeared to maintain or enhance epidemics. Further research is needed to investigate the relation of dengue virus transovarial transmission with DHF endemicity in Jakarta.

  9. Comportamento de formas imaturas de Aedes aegypti, no litoral do Estado de São Paulo

    Directory of Open Access Journals (Sweden)

    Carmen Moreno Glasser

    2011-06-01

    Full Text Available INTRODUÇÃO: Em região de alta incidência de dengue, no litoral do Estado de São Paulo, selecionaram-se 9 áreas, com objetivo de avaliar o comportamento de formas imaturas de Aedes aegypti. MÉTODOS: As 9 áreas foram agrupadas em 4 estratos, diferenciados pelo uso e ocupação do solo. Foram coletadas larvas e pupas numa amostra de cerca de 500 imóveis em cada área. RESULTADOS: Apesar do pneu e lona apresentarem as maiores taxas de positividade para Aedes aegypti, o ralo, juntamente com outros recipientes fixos nas edificações foram altamente predominantes entre os recipientes positivos (32 a 76% dos recipientes positivos. As áreas coletivas de prédios e os imóveis não residenciais de grande porte apresentaram as maiores taxas de positividade para Aedes aegypti enquanto os apartamentos, as menores. Os níveis de infestação foram maiores na área residencial com predominância de prédios de apartamentos, onde 76% dos criadouros detectados foram recipientes fixos nas edificações. CONCLUSÕES: Esses conhecimentos são importantes subsídios para a estratégia de controle, pois reforçam a necessidade de atenção especial para determinados tipos de imóveis, bem como da adequação da norma técnica de ralo de água pluvial e da melhoria de manutenção das edificações. Além disso, são necessárias observações sistemáticas que permitam acompanhar a dinâmica de ocupação de diferentes imóveis e recipientes por Aedes aegypti e a incorporação desses conhecimentos nas ações de controle do vetor na região.

  10. "As moedas dos índios": um estudo de caso sobre os significados do patrimônio arqueológico para os moradores da Vila de Joanes, ilha de Marajó, Brasil

    Directory of Open Access Journals (Sweden)

    Marcia Bezerra

    Full Text Available O artigo apresenta os resultados iniciais do projeto de Arqueologia Pública conduzido pela autora em Joanes - uma pequena vila de pescadores na ilha de Marajó, estado do Pará, Brasil -, considerando as ruínas de um sítio histórico (PA-JO-46 associado a uma missão religiosa instalada na vila por volta da segunda metade do século XVII. As reflexões sobre as percepções das comunidades locais acerca do sítio sublinham o entendimento da lógica subjacente ao ato de colecionar artefatos - prática local comum - e à formação de pequenas 'coleções domésticas'. Meu argumento é de que o colecionamento, nesses contextos, não pode ser classificado como ato de destruição, mas como uma forma singular de fruição do passado e do patrimônio. Acredito que essa discussão contribua para a compreensão das relações entre comunidades de pequena escala e o patrimônio arqueológico na Amazônia.

  11. Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection.

    Directory of Open Access Journals (Sweden)

    Michael J Conway

    2016-09-01

    Full Text Available Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV types 1-4. Ae. aegypti transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. Ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. Previously, we showed that Ae. aegypti salivary gland extracts (SGEs enhanced dissemination of DENV to draining lymph nodes. In contrast, HPLC-fractionation revealed that some SGE components inhibited infection. Here, we show that D7 proteins are enriched in HPLC fractions that are inhibitory to DENV infection, and that recombinant D7 protein can inhibit DENV infection in vitro and in vivo. Further, binding assays indicate that D7 protein can directly interact with DENV virions and recombinant DENV envelope protein. These data reveal a novel role for D7 proteins, which inhibits arbovirus transmission to vertebrates through a direct interaction with virions.

  12. Susceptibility of Aedes aegypti pupae to neem seed kernal extracts ...

    African Journals Online (AJOL)

    Azadirachta indica) seed kernel extracts (NSKE) on Aedes aegypti. The neem seed kernel powder was sequentially extracted with hexane, benzene, ethyl acetate, acetone, DMSO, 2-propanol, ethanol, methanol and dstiledwater.

  13. Ciclos de vida comparados de Aedes aegypti (Diptera, Culicidae do semiárido da Paraíba

    Directory of Open Access Journals (Sweden)

    Francisco P. de Castro Jr.

    2013-06-01

    Full Text Available O presente trabalho teve como objetivo comparar os ciclos de vida entre amostras de populações de Aedes aegypti (Linnaeus, 1762 coletadas em dez municípios localizados no semiárido paraibano. Os ciclos de vida foram estudados a uma temperatura de 26 ± 2ºC, umidade relativa de 60 ± 10% e fotofase de 12 horas. Diariamente foram avaliados os períodos de desenvolvimentos e as viabilidades das fases de ovo, larva e pupa, bem como a razão sexual, longevidade, tamanho e fecundidade dos adultos. Foi realizada uma análise de agrupamento, utilizando-se uma matriz de distância euclidiana através do método da média não-ponderada. As durações e viabilidades para as fases de ovo, larva e pupa apresentaram respectivamente, uma variação média de 3,7 a 4,7 dias e 82,8% a 97,7%, 9,1 a 10,8 dias e 91,2% a 99,2% e de 2,1 a 2,5 dias e 93,5% a 98,4%. O comprimento alar foi de 5,13 a 5,34 mm para as fêmeas e de 4,18 a 4,25 mm para os machos. A menor fecundidade (153,6 ovos/fêmea ocorreu na população de A. aegypti oriunda de Pedra Lavrada, enquanto que a maior fecundidade (310,6 ovos/fêmea foi constatada para A. aegypti de Campina Grande. A análise de agrupamento com base na similaridade dos dados biológicos revelou a formação de dois grandes grupos distintos, onde as populações de A. aegypti de Serra Branca e Cuité apresentam maior similaridade entre si. As diferenças de ciclos biológicos verificadas entre as populações de A. aegypti demonstra a capacidade dessa espécie de sofrer variações na sua biologia e se adaptar às diferentes condições ambientais, favorecendo a permanência deste inseto nessas áreas com aumento do risco de transmissão do vírus da dengue.

  14. History of domestication and spread of Aedes aegypti - A Review

    OpenAIRE

    Jeffrey R Powell; Walter J Tabachnick

    2013-01-01

    The adaptation of insect vectors of human diseases to breed in human habitats (domestication) is one of the most important phenomena in medical entomology. Considerable data are available on the vector mosquito Aedes aegypti in this regard and here we integrate the available information including genetics, behaviour, morphology, ecology and biogeography of the mosquito, with human history. We emphasise the tremendous amount of variation possessed by Ae. aegypti for virtually all traits consid...

  15. Larvicidal activity of Cybistax antisyphilitica against Aedes aegypti larvae.

    Science.gov (United States)

    Rodrigues, A M S; de Paula, J E; Roblot, F; Fournet, A; Espíndola, L S

    2005-12-01

    The larvicidal activity against Aedes aegypti larvae of a stem wood hexane extract of Cybistax antisyphilitica was evaluated. Bioassay-guided fractionation of the crude extract, monitored by larvicidal assay, led to the isolation of a natural quinone identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1.4-naphthoquinone (lapachol). This compound was quite potent against A. aegypti larvae (LC50 26.3 microg/ml).

  16. Occurrence and distribution of the exotic lizard Hemidactylus mabouia Moreau de Jonnès, 1818 in Ilha Grande, RJ, Brazil

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    CFD Rocha

    Full Text Available The gekkonid lizard Hemidactylus mabouia is an exotic species in Brazil and is found in different ecosystems. This species was recorded at Ilha Grande, RJ, one of the largest insular remains of the Atlantic Rainforest in Brazil. In this study, the occurrence of H. mabouia was determined throughout the island, including the rain forest, restinga and anthropic environments. We used the active search method in points along 19 trails that surround Ilha Grande. At each regular interval of 100 m, we searched for the presence of H. mabouia. The species was recorded in a total of 100 points among the 719 sampled and, in all cases, the occurrence of the lizard corresponded to points located in anthropic or perianthropic areas. As most of Ilha Grande is covered by dense tropical rain forest, we believe this has restricted the invasion of H. mabouia in natural environments within the island.

  17. Análise das causas da deterioração dos postes de madeira da Ilha do Mel, Paraná, Brasil

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    Marina Mieko Nishidate Kumode

    2013-03-01

    Full Text Available http://dx.doi.org/10.5902/198050988455Objetivou-se a quantificação química dos preservantes, identificação anatômica da espécie florestal e dos principais agentes deterioradores dos postes de madeira de uma rede de distribuição de energia elétrica da Ilha do Mel, no litoral do Estado do Paraná. Além disso, foram propostas formas de prolongar a vida útil do poste em uso. Os postes foram caracterizados anatomicamente e a identificação e quantificação química foram realizadas por meio de análise colorimétrica e absorção atômica. Avaliou-se a retenção média amostral pelo teste t de Student e na comparação entre os grupos foi aplicado o teste de Fisher. A identificação dos agentes deterioradores foi realizada de acordo com a inspeção visual, com o auxílio de pequenas ferramentas. As análises anatômicas identificaram os postes como sendo da espécie Eucalyptus grandis. Os testes químicos qualitativos revelaram a presença de preservante arseniato de cobre cromatado (CCA e borato de cobre cromatado (CCB tendo os índices de retenção para CCA apresentado valores inferiores ao mínimo recomendado. Os resultados da inspeção visual indicaram ataques por pica-paus, fungos e cupins. Para o modelo de gerenciamento foram propostas ações integradas desde o recebimento da matéria-prima, monitoramento e manutenção dos postes.

  18. COMPARTIMENTAÇÃO MORFOLÓGICA DA MARGEM LESTE DA ILHA DE MARAJÓ: ZONA COSTEIRA DOS MUNICÍPIOS DE SOURE E SALVATERRA – ESTADO DO PARÁ

    Directory of Open Access Journals (Sweden)

    Carmena Ferreira de França

    2006-11-01

    Full Text Available A compartimentação morfológica da zona costeira de Soure e Salvaterra, margem leste da Ilha de Marajó (PA; baseia-se em dados de sensores remotos, nas características geológicas, topográficas e vegetacionais levantadas em trabalhos de campo e na revisão da literatura sobre a região do Golfão Amazônico. A área de estudo subdivide-se em duas grandes unidades: planalto costeiro e planície costeira. O planalto costeiro é constituído por sedimentos areno-argilosos terciários do Grupo Barreiras/Pós-Barreiras, correspondendo a um relevo aplainado com suaves ondulações e cotas topográficas acima de 5 m (baixo platô. A cobertura vegetal apresenta matas secundárias, capoeiras e cerrados. A planície costeira é constituída por sedimentos lamosos e arenosos holocênicos, depositados sob influência de correntes de maré e ondas, apresentando cotas topográficas abaixo de 5 m. A cobertura vegetal é representada por mangues, campos inundáveis e restingas. As sub- unidades da planície costeira são: paleocanal, terraço arenoso, planície lamosa de supramaré, planície lamosa de intermaré, cordões arenosos antigos, cordões arenosos de dunas e praias atuais, canais de maré e deltas de maré vazante.

  19. Comportamento de formas imaturas de Aedes aegypti, no litoral do Estado de São Paulo Behavior of immatures Aedes aegypti in the coast State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Carmen Moreno Glasser

    2011-06-01

    Full Text Available INTRODUÇÃO: Em região de alta incidência de dengue, no litoral do Estado de São Paulo, selecionaram-se 9 áreas, com objetivo de avaliar o comportamento de formas imaturas de Aedes aegypti. MÉTODOS: As 9 áreas foram agrupadas em 4 estratos, diferenciados pelo uso e ocupação do solo. Foram coletadas larvas e pupas numa amostra de cerca de 500 imóveis em cada área. RESULTADOS: Apesar do pneu e lona apresentarem as maiores taxas de positividade para Aedes aegypti, o ralo, juntamente com outros recipientes fixos nas edificações foram altamente predominantes entre os recipientes positivos (32 a 76% dos recipientes positivos. As áreas coletivas de prédios e os imóveis não residenciais de grande porte apresentaram as maiores taxas de positividade para Aedes aegypti enquanto os apartamentos, as menores. Os níveis de infestação foram maiores na área residencial com predominância de prédios de apartamentos, onde 76% dos criadouros detectados foram recipientes fixos nas edificações. CONCLUSÕES: Esses conhecimentos são importantes subsídios para a estratégia de controle, pois reforçam a necessidade de atenção especial para determinados tipos de imóveis, bem como da adequação da norma técnica de ralo de água pluvial e da melhoria de manutenção das edificações. Além disso, são necessárias observações sistemáticas que permitam acompanhar a dinâmica de ocupação de diferentes imóveis e recipientes por Aedes aegypti e a incorporação desses conhecimentos nas ações de controle do vetor na região.INTRODUCTION: In a region of high dengue incidence, on the coast of the State of São Paulo, 9 areas were selected to evaluate the behavior of immature Aedes aegypti. METHODS: The 9 areas were grouped into 4 strata according to soil use and occupation. Larvae and pupas were collected in a sample of approximately 500 buildings in each area. RESULTS: Although tires and canvas presented the highest positive rates for

  20. Assessment of Local Mosquito Species Incriminates Aedes aegypti as the Potential Vector of Zika Virus in Australia.

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    Sonja Hall-Mendelin

    2016-09-01

    Full Text Available Within the last 10 years Zika virus (ZIKV has caused unprecedented epidemics of human disease in the nations and territories of the western Pacific and South America, and continues to escalate in both endemic and non-endemic regions. We evaluated the vector competence of Australian mosquitoes for ZIKV to assess their potential role in virus transmission.Mosquitoes were exposed to infectious blood meals containing the prototype African ZIKV strain. After 14 days incubation at 28°C and high relative humidity, infection, dissemination and transmission rates were assessed. Infection in Culex annulirostris and Cx. sitiens could not be detected. 8% of Cx. quinquefasciatus were infected, but the virus did not disseminate in this species. Despite having infection rates > 50%, Aedes notoscriptus and Ae. vigilax did not transmit ZIKV. In contrast, Ae. aegypti had infection and transmission rates of 57% and 27%, respectively. In susceptibility trials, the virus dose required to infect 50% (ID50 of Ae. aegypti was106.4 tissue culture infectious dose50 (TCID50/mL. Additionally, a threshold viral load within the mosquito of at least 105.1 TCID50 equivalents/mL had to be reached before virus transmission occurred.We confirmed Ae. aegypti to be the most likely mosquito vector of ZIKV in Australia, although the restricted distribution of this species will limit the receptive zone to northern Queensland where this species occurs. Importantly, the role in ZIKV transmission of Culex and other Aedes spp. tested will be negligible. Despite being the implicated vector, the relatively high ID50 and need for a high titer disseminated infection in Ae. aegypti suggest that high mosquito population densities will be required to facilitate epidemic ZIKV transmission among the currently immunologically naïve human population in Australia.

  1. Assessment of Local Mosquito Species Incriminates Aedes aegypti as the Potential Vector of Zika Virus in Australia.

    Science.gov (United States)

    Hall-Mendelin, Sonja; Pyke, Alyssa T; Moore, Peter R; Mackay, Ian M; McMahon, Jamie L; Ritchie, Scott A; Taylor, Carmel T; Moore, Frederick A J; van den Hurk, Andrew F

    2016-09-01

    Within the last 10 years Zika virus (ZIKV) has caused unprecedented epidemics of human disease in the nations and territories of the western Pacific and South America, and continues to escalate in both endemic and non-endemic regions. We evaluated the vector competence of Australian mosquitoes for ZIKV to assess their potential role in virus transmission. Mosquitoes were exposed to infectious blood meals containing the prototype African ZIKV strain. After 14 days incubation at 28°C and high relative humidity, infection, dissemination and transmission rates were assessed. Infection in Culex annulirostris and Cx. sitiens could not be detected. 8% of Cx. quinquefasciatus were infected, but the virus did not disseminate in this species. Despite having infection rates > 50%, Aedes notoscriptus and Ae. vigilax did not transmit ZIKV. In contrast, Ae. aegypti had infection and transmission rates of 57% and 27%, respectively. In susceptibility trials, the virus dose required to infect 50% (ID50) of Ae. aegypti was106.4 tissue culture infectious dose50 (TCID50)/mL. Additionally, a threshold viral load within the mosquito of at least 105.1 TCID50 equivalents/mL had to be reached before virus transmission occurred. We confirmed Ae. aegypti to be the most likely mosquito vector of ZIKV in Australia, although the restricted distribution of this species will limit the receptive zone to northern Queensland where this species occurs. Importantly, the role in ZIKV transmission of Culex and other Aedes spp. tested will be negligible. Despite being the implicated vector, the relatively high ID50 and need for a high titer disseminated infection in Ae. aegypti suggest that high mosquito population densities will be required to facilitate epidemic ZIKV transmission among the currently immunologically naïve human population in Australia.

  2. Diversidade e estrutura genética de populações de Varronia curassavica Jacq. em restingas da Ilha de Santa Catarina

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    M.P. HOELTGEBAUM

    2015-01-01

    Full Text Available RESUMO Varronia curassavica Jacq. (Boraginaceae está presente na vegetação de restinga e apresenta relevantes propriedades medicinais. A espécie é explorada especialmente por comunidades locais e pela indústria farmacêutica, porém, carece de informações ecológicas e genéticas a seu respeito. Nesse contexto, o estudo foi conduzido com o objetivo de caracterizar a diversidade genética de três populações de V. curassavica em áreas de restinga na Ilha de Santa Catarina. Foram coletadas folhas de 50 indivíduos adultos em cada uma das três áreas de estudo e as frequências alélicas das populações foram obtidas a partir de 14 locos alozímicos. Foram encontrados 25 alelos distintos nas três populações, sendo dois alelos exclusivos. As populações apresentaram diversidade genética média de 0,111 e índice de fixação médio de -0,060 (-0,273 até 0,222. Os níveis de diversidade são intermediários, semelhantes aos exibidos por espécies da mesma família ou de características ecológicas semelhantes. Os índices de fixação foram todos significativos e discrepantes entre as populações, sendo que duas delas apresentaram excesso de heterozigotos. A divergência genética interpopulacional foi significativa e igual a 0,079, considerada moderada e sugerindo efeitos de subdivisão populacional. Os níveis de diversidade genética encontrados e a redução populacional causada pela redução e fragmentação dos habitats em que a espécie ocorre sugerem medidas de conservação ex situ e demandam maior rigor na proteção legal de áreas de proteção permanente.

  3. Study of the dispersion of radionuclides in the Ilha Grande Bay - RJ

    International Nuclear Information System (INIS)

    Gomes, Franciane Martins de Carvalho

    2010-01-01

    The present work aimed the study of the dispersion profiles of radionuclides released in liquid radioactive effluents from the Central Nuclear Almirante Alvaro Alberto (CNAAA), in the Ilha Grande Bay, located in Angra dos Reis, RJ. Taking the overall activity released by liquid effluents discharge from the CNAAA in the Piraquara de Fora Bay as a reference parameter to the dilution rates scanning, the accordance level between the achieved 3 H results (1 :16) and those reported by Franklin (1 :20) can be seen as relevant. The post-1965 137 Cs concentration raise can be regarded as a consequence of the operational start up in the CNAAA, being the Ribeira Bay considered its preferential uptake, from the plume displacement high tide, as well as its retention, due to local deposition favourable trends. The evaluation of the prevailing mechanism on long half-life radio isotopes dispersion pointed out its dependence on meteorological condition parameters, during the sampling procedures. If diffusion prevailingness occurs, both 223 Ra and 224 Ra distribution pathways result in diffusion coefficients ranging from 20,12 ± 3,81 - 21,07 ± 3,91 km 2 .d -1 to a 228 Ra offshore flux and time of residence of, respectively, 3,18 ± 1,01 x 108 Bq.d-1 and 25,35 ± 0,45 days. On the other hand, an uppermost advection process provided 228 Ra offshore flux and time of residence of, respectively, 2,26 ± 0,424 x 10 8 Bq.d -1 and 13,08 ± 0,21 days. (author)

  4. Study of the hydrodynamic circulation and transport of radionuclides in the Ilha Grande Bay -RJ

    International Nuclear Information System (INIS)

    Franklin, Mariza Ramalho

    2001-01-01

    The Almirante Alvaro Alberto Nuclear Center (CNAAA), located at the city of Rio de Janeiro and the liquid effluents are released into the Ilha Grande Bay (BIG). The objective of this work was to simulate mathematically the dispersion of 3 H and 137 Cs present in the liquid effluents that are routinely released into the environment, and in this way contribute to the improvement of the radiological impact assessment associated to these releases. The hydrodynamic circulation pattern of the bay and the transport of radionuclides were simulated by means of numerical modeling techniques by the computational system SisBAHIA (Sistema Base Hidrodinâmica e Ambiental). The results indicate that the local circulation pattern is mainly driven by the propagation of the tidal wave, and is characterized by low current velocities of about 0.1 m.s -1 . The wind stress (normal or extreme ones ) over the free surface does not alter the local circulation pattern significantly. The effluents are released in a rate of about 120 m 3 .s. -1 . As a result, the local hydrodynamics is markedly altered by means of the formation of a huge vortices close to the release area. According to the annual amount of the released radionuclides predicted in the Nuclear Licensing Document, and considering a continuous release it could be predicted that the radionuclide plume would present a low mobility, with most of the activity concentration amount being restricted close to the source. The activity concentration distribution reach the transportation steady state by the end of the 51 st day of simulation. A conservative behavior of both radionuclides in the water column was assumed in the performed simulation. (author)

  5. Utilização de sensoriamento remoto em análises de albedo e temperatura de superfície em Londrina - PR: contribuições para estudos de ilha de calor urbana

    Directory of Open Access Journals (Sweden)

    Nancy Laura Rios Gamarra

    2014-12-01

    Full Text Available Este trabalho avaliou o albedo (αs e a temperatura de superfície continental (Tsup provenientes de sensoriamento remoto, e a temperatura do ar (Tar medida in situ no intuito de caracterizar a Ilha de Calor Urbana (ICU na cidade de Londrina (Paraná. Foram utilizados dados de αs e Tsup obtidos do sensor MODIS, e medições in situ de Tar realizadas entre junho e agosto de 2011 por sensores meteorológicos instalados em 13 locais na cidade. Os dados de αs e Tsup foram utilizados para calcular um índice de urbanização (IU na região e posteriormente comparado com Tar. A análise dos dados de αs e Tsup foi consistente com as medições in situ, evidenciando sua aplicabilidade na avaliação espaço-temporal de fenômenos acoplados continente-atmosfera, tais como a ICU. A mancha urbana da cidade de Londrina apresentou valores de IU compreendido entre 18 e 20, bem maiores que as áreas mais vegetadas (14 - 16. Nesse sentido, o IU demonstrou-se ser uma metodologia útil para representar a evolução espaço-temporal de áreas urbanas, o que pode propiciar uma avaliação detalhada do impacto da mudança de cobertura de solo no clima local e regional. Além disso, os resultados indicam que dados obtidos via satélite podem auxiliar grandemente na quantificação da ICU, quando dados in situ estiverem indisponíveis.

  6. ser precoce na intervenção

    OpenAIRE

    Bernardo, Ana Carolina Couto

    2013-01-01

    Dissertação apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências da Educação: Educação Especial, área de especialização em Domínio da Intervenção Precoce na Infância Este estudo centra-se na implementação de uma Escola de Mães, programa de educação parental realizado em seis sessões na freguesia de Calhetas, ilha de S. Miguel, e na avaliação comparativa, anterior e posterior ao programa, das três mães participantes no que...

  7. Gustatory receptor neuron responds to DEET and other insect repellents in the yellow-fever mosquito, Aedes aegypti

    Science.gov (United States)

    Sanford, Jillian L.; Shields, Vonnie D. C.; Dickens, Joseph C.

    2013-03-01

    Three gustatory receptor neurons were characterized for contact chemoreceptive sensilla on the labella of female yellow-fever mosquitoes, Aedes aegypti. The neuron with the smallest amplitude spike responded to the feeding deterrent, quinine, as well as N, N-diethyl-3-methylbenzamide and other insect repellents. Two other neurons with differing spikes responded to salt (NaCl) and sucrose. This is the first report of a gustatory receptor neuron specific for insect repellents in mosquitoes and may provide a tool for screening chemicals to discover novel or improved feeding deterrents and repellents for use in the management of arthropod disease vectors.

  8. Paralelos Possíveis: Paranapiacaba e Ilha Solteira sob a perspectiva dos princípios do Urbanismo Moderno

    Directory of Open Access Journals (Sweden)

    Fernanda Figueiredo D'Agostini

    2015-07-01

    Full Text Available A análise da morfologia urbana das Vilas Ferroviária de Paranapiacaba e Operária de Ilha Solteira segundo os conceitos do urbanismo funcional difundido por Le Corbusier constitui o foco deste artigo, que objetiva mostrar as características comuns de dois planejamentos distintos temporalmente, mas com elementos estruturantes de organização semelhantes. Embasado nos fundamentos da Carta de Atenas para o Urbanismo Moderno, que se apoiavam nas quatro funções básicas: habitar, trabalhar, recrear-se e circular, sendo a habitação considerada o ponto de articulação de todas as medidas e o zoneamento das cidades como ordenador o território urbano. Identificouse, que seus planejamentos detiveram preocupações claras em relação ao seu território, estabelecendo etapas temporais de desenvolvimento, com estudos de recursos naturais e sítio anteriores a implantação, bem como definição de etapas para sua execução, assemelhando-se as recomendações da Carta de Atenas. Palavras-chave: Ilha Solteira; Le Corbusier; Morfologia Urbana; Paranapiacaba; Urbanismo Moderno.

  9. DIVERSITY OF ARBUSCULAR MYCORRHIZAL FUNGI ALONG A SAND DUNE STABILIZATION GRADIENT: A CASE STUDY AT PRAIA DE JOAQUINA, ILHA DE SANTA CATARINA, SOUTH BRAZIL

    Science.gov (United States)

    Species diversity of abuscular mycorrhizal fungi (AMF) was assessed along a dunes stabilization gradient (embyonic dune, foredune and fixed dune) at Praia da Joaquina (Joaquina Beach), Ilha de Santa Catarina. These dunes served as a case study to assess whether diversity and myc...

  10. Swarming Mechanisms in the Yellow Fever Mosquito: Aggregation Pheromones are Involved in the Mating Behavior of Aedes aegypti

    Science.gov (United States)

    2014-12-01

    behavior of Aedes aegypti Emadeldin Y. Fawaz1, Sandra A. Allan2, Ulrich R. Bernier2, Peter J. Obenauer3, and Joseph W. Diclaro II1 1Vector Biology... Aedes aegypti swarming behavior and identified associated chemical cues. Novel evidence is provided that Ae. aegypti females aggregate by means of...the isolated aggregation pheromones in controlling Ae. aegypti. Journal of Vector Ecology 39 (2): 347-354. 2014. Keyword Index: Aedes aegypti, swarm

  11. Potential use of Piper nigrum ethanol extract against pyrethroid-resistant Aedes aegypti larvae Utilização em potencial do extrato alcoólico de Piper nigrum como larvicida em Aedes aegypti resistente a piretróides

    Directory of Open Access Journals (Sweden)

    Naomi Kato Simas

    2007-08-01

    Full Text Available Fractionation of Piper nigrum ethanol extract, biomonitored by assays on pyrethroid-resistant Aedes aegypti larvae yielded isolation of the larvicidal amides piperolein-A and piperine. Comparing LC50 values, the ethanol extract (0. 98 ppm was the most toxic, followed by piperolein-A (1. 46ppm and piperine (1. 53ppm.O fracionamento do extrato etanólico de Piper nigrum biomonitorado por ensaios em larvas de Aedes aegypti resistentes a piretróides resultou no isolamento das amidas larvicidas piperoleína-A e piperina. Comparando-se os valores de CL50, o extrato etanólico (0. 98ppm foi o mais tóxico, seguido pela piperoleína-A (1. 46ppm e piperina (1. 53ppm.

  12. Estimativa das máximas pistas de vento no reservatório da barragem de Ilha Solteira, Estado de São Paulo = Estimation of the maximum wind fetches in Ilha Solteira, state of São Paulo, reservoir

    Directory of Open Access Journals (Sweden)

    Marcelo Marques

    2007-01-01

    Full Text Available Grande parte dos métodos empregados na estimativa da altura de ondas,geradas pela ação do vento, necessita da determinação do comprimento da pista sobre a qual o vento atua. A escolha do método depende da natureza do corpo de água, podendo ser aplicável a áreas oceânicas ou a corpos d’água interiores, como baías, lagos e reservatórios.Uma das soluções rotineiras tem sido a aplicação direta do método validado em águas oceânicas para águas interiores sem levar, portanto, em consideração, o efeito de restrição oferecido pelas margens. Não obstante, a excessiva quantidade de operações do métodoaplicado a corpos de água interiores (onde a margem é condição de restrição, pode, se não usado uma base gráfica suficientemente precisa da margem acrescido da subjetividade do operador, não assegurar um grau de precisão satisfatório. Assim exposto, esta comunicação científica traz à luz esta discussão, propondo uma solução clássica baseada na aplicação do método adequado a águas interiores (Saville et al., 1954, via processamento automatizado.Para tanto, foi desenvolvido um programa em linguagem computacional AutoLISP. A aplicação do programa determinou as máximas pistas de vento no reservatório da barragem de Ilha Solteira, Estado de São Paulo, como estando entre 9,5 e 12,5 km, contrariamente aum estudo anterior que havia previsto pistas bem superiores (fator da ordem de 3.Most methods employed to estimate the height of waves generatedby the wind require the surface length over which the wind blows. The choice of method depends on the nature of the water body, being applicable to ocean areas or interior water bodies, such as bays, lakes and reservoirs. One of the usual solutions has been the directusage of the method validated in ocean waters for interior waters, therefore, not taking into consideration the effect of the restriction imposed by the shores. Nevertheless, the excessivequantity of

  13. Mathematical model of temephos resistance in Aedes aegypti mosquito population

    Science.gov (United States)

    Aldila, D.; Nuraini, N.; Soewono, E.; Supriatna, A. K.

    2014-03-01

    Aedes aegypti is the main vector of dengue disease in many tropical and sub-tropical countries. Dengue became major public concern in these countries due to the unavailability of vaccine or drugs for dengue disease in the market. Hence, the only way to control the spread of DF and DHF is by controlling the vectors carrying the disease, for instance with fumigation, temephos or genetic manipulation. Many previous studies conclude that Aedes aegypti may develop resistance to many kind of insecticide, including temephos. Mathematical model for transmission of temephos resistance in Aedes aegypti population is discussed in this paper. Nontrivial equilibrium point of the system and the corresponding existence are shown analytically. The model analysis have shown epidemiological trends condition that permits the coexistence of nontrivial equilibrium is given analytically. Numerical results are given to show parameter sensitivity and some cases of worsening effect values for illustrating possible conditions in the field.

  14. Effect of temperature on the population dynamics of Aedes aegypti

    Science.gov (United States)

    Yusoff, Nuraini; Tokachil, Mohd Najir

    2015-10-01

    Aedes aegypti is one of the main vectors in the transmission of dengue fever. Its abundance may cause the spread of the disease to be more intense. In the study of its biological life cycle, temperature was found to increase the development rate of each stage of this species and thus, accelerate the process of the development from egg to adult. In this paper, a Lefkovitch matrix model will be used to study the stage-structured population dynamics of Aedes aegypti. In constructing the transition matrix, temperature will be taken into account. As a case study, temperature recorded at the Subang Meteorological Station for year 2006 until 2010 will be used. Population dynamics of Aedes aegypti at maximum, average and minimum temperature for each year will be simulated and compared. It is expected that the higher the temperature, the faster the mosquito will breed. The result will be compared to the number of dengue fever incidences to see their relationship.

  15. The global compendium of Aedes aegypti and Ae. albopictus occurrence

    Science.gov (United States)

    Kraemer, Moritz U. G.; Sinka, Marianne E.; Duda, Kirsten A.; Mylne, Adrian; Shearer, Freya M.; Brady, Oliver J.; Messina, Jane P.; Barker, Christopher M.; Moore, Chester G.; Carvalho, Roberta G.; Coelho, Giovanini E.; van Bortel, Wim; Hendrickx, Guy; Schaffner, Francis; Wint, G. R. William; Elyazar, Iqbal R. F.; Teng, Hwa-Jen; Hay, Simon I.

    2015-07-01

    Aedes aegypti and Ae. albopictus are the main vectors transmitting dengue and chikungunya viruses. Despite being pathogens of global public health importance, knowledge of their vectors’ global distribution remains patchy and sparse. A global geographic database of known occurrences of Ae. aegypti and Ae. albopictus between 1960 and 2014 was compiled. Herein we present the database, which comprises occurrence data linked to point or polygon locations, derived from peer-reviewed literature and unpublished studies including national entomological surveys and expert networks. We describe all data collection processes, as well as geo-positioning methods, database management and quality-control procedures. This is the first comprehensive global database of Ae. aegypti and Ae. albopictus occurrence, consisting of 19,930 and 22,137 geo-positioned occurrence records respectively. Both datasets can be used for a variety of mapping and spatial analyses of the vectors and, by inference, the diseases they transmit.

  16. Stage-Structured Population Dynamics of AEDES AEGYPTI

    Science.gov (United States)

    Yusoff, Nuraini; Budin, Harun; Ismail, Salemah

    Aedes aegypti is the main vector in the transmission of dengue fever, a vector-borne disease affecting world population living in tropical and sub-tropical countries. Better understanding of the dynamics of its population growth will help in the efforts of controlling the spread of this disease. In looking at the population dynamics of Aedes aegypti, this paper explored the stage-structured modeling of the population growth of the mosquito using the matrix population model. The life cycle of the mosquito was divided into five stages: eggs, larvae, pupae, adult1 and adult2. Developmental rates were obtained for the average Malaysian temperature and these were used in constructing the transition matrix for the matrix model. The model, which was based only on temperature, projected that the population of Aedes aegypti will blow up with time, which is not realistic. For further work, other factors need to be taken into account to obtain a more realistic result.

  17. METROPOLlZAÇÃO NA ILHA OE SÃO LUIS E SEUS IMPACTOS SANITÁRIOS E AMBIENTAIS

    Directory of Open Access Journals (Sweden)

    Lúcio Antônio Alves de Macêdo

    2012-01-01

    Full Text Available The paper presents aspects 01 the Environmental Sanitation considered on the island 01 Maranhão, lrom a historical perspective, restoring the role 01 sanitation in improving the quality 01 lile and highlighting the low leveis 01 coverage 01 water supply (56% and sanitation (12%, and the precariousness 01 the disposal 01 solid waste in the municipalities 01 São Luis, Paço do Lumiar, Raposa and São José de Ribamar. ln particular there is no more available land lar construction 01 landlills, because 01 environmental implications in areas 01 watershed protection. As a result 01 the studies showed that water pollution in major rivers 01 the island with the pollution load 0160 ton / day, committing the multiple uses 01 the river Anil, Bacanga, Patience and Tibiri, as well as high rates 01 inlectious diseases and child mortality the precariousness 01 Sanitation problems associated with the generation 01 700 t / d 01 waste. ln conclusion the study shows the need lar the implementation 01 environmental planning, ordering the use 01 space and adopting techniques 01 control and environmental health lar the population.

  18. DISTRIBUIÇÃO ESPAÇO-TEMPORAL DA PLUVIOSIDADE NA ILHA DO MARANHÃO NO ANO DE 2016

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    Juarez Mota Pinheiro

    2017-08-01

    Esta investigación identificó espacialmente y temporalmente la dinámica de frecuencia acumulada de las lluvias en la Isla de Maranhão durante el año 2016. Utilizando la base de datos del Centro Nacional de Monitoreo y Alerta de Desastres Naturales - CEMADEN y del Instituto Nacional de Meteorología - INMET Se recogieron datos pluviométricos de 13 (trece estaciones automatizadas distribuidas por toda la isla. Después del procesamiento de los datos por el software Golden Surfer 13, utilizando el método de interpolación de riego, se produjeron cartográficamente mapas representativos de la distribución mensual, estacional y anual de las lluvias. Con el resultado de la investigación fue posible caracterizar que la mayor cantidad de lluvias anuales ocurre en el sector sur / suroeste de la Isla, abarcando toda el área del distrito industrial de São Luís, y la menor cantidad de lluvias ocurre al norte / noreste de la Isla, por Todo el municipio de Raposa, presentando una diferencia de variación del 39,19% del total anual. Otra conclusión que la investigación logró identificar es que en la distribución de las lluvias en el año 2016, en una gran franja urbana de São Luís, envolviendo los barrios de Cohama, Viñedos, Bequimão, Cohajap y parte del Turu, se presentaron como los barrios de menor volumen de pluviosidad dentro del municipio de São Luís y el área en el entorno del Aeropuerto hacia el distrito industrial es la que presentó los mayores volúmenes pluviométricos anuales. También fue posible inferir que los vientos en la isla, en el año 2016, poseen una predominancia de dirección Este-Nordeste (ENE y que esta dirección está influenciando en la determinación de su espacialidad pluviométrica. Palabras clave: Isla de Maranhão; Pluviosidad; Distribución Espacio-temporal.

  19. Assessing the effects of Aedes aegypti kdr mutations on pyrethroid resistance and its fitness cost.

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    Luiz Paulo Brito

    Full Text Available Pyrethroids are the most used insecticide class worldwide. They target the voltage gated sodium channel (NaV, inducing the knockdown effect. In Aedes aegypti, the main dengue vector, the AaNaV substitutions Val1016Ile and Phe1534Cys are the most important knockdown resistance (kdr mutations. We evaluated the fitness cost of these kdr mutations related to distinct aspects of development and reproduction, in the absence of any other major resistance mechanism. To accomplish this, we initially set up 68 crosses with mosquitoes from a natural population. Allele-specific PCR revealed that one couple, the one originating the CIT-32 strain, had both parents homozygous for both kdr mutations. However, this pyrethroid resistant strain also presented high levels of detoxifying enzymes, which synergistically account for resistance, as revealed by biological and biochemical assays. Therefore, we carried out backcrosses between CIT-32 and Rockefeller (an insecticide susceptible strain for eight generations in order to bring the kdr mutation into a susceptible genetic background. This new strain, named Rock-kdr, was highly resistant to pyrethroid and presented reduced alteration of detoxifying activity. Fitness of the Rock-kdr was then evaluated in comparison with Rockefeller. In this strain, larval development took longer, adults had an increased locomotor activity, fewer females laid eggs, and produced a lower number of eggs. Under an inter-strain competition scenario, the Rock-kdr larvae developed even slower. Moreover, when Rockefeller and Rock-kdr were reared together in population cage experiments during 15 generations in absence of insecticide, the mutant allele decreased in frequency. These results strongly suggest that the Ae. aegypti kdr mutations have a high fitness cost. Therefore, enhanced surveillance for resistance should be priority in localities where the kdr mutation is found before new adaptive alleles can be selected for diminishing the

  20. History of domestication and spread of Aedes aegypti - A Review

    Directory of Open Access Journals (Sweden)

    Jeffrey R Powell

    2013-01-01

    Full Text Available The adaptation of insect vectors of human diseases to breed in human habitats (domestication is one of the most important phenomena in medical entomology. Considerable data are available on the vector mosquito Aedes aegypti in this regard and here we integrate the available information including genetics, behaviour, morphology, ecology and biogeography of the mosquito, with human history. We emphasise the tremendous amount of variation possessed by Ae. aegypti for virtually all traits considered. Typological thinking needs to be abandoned to reach a realistic and comprehensive understanding of this important vector of yellow fever, dengue and Chikungunya.

  1. Gestão, governança e uso das águas no Brasil e em Singapura: um estudo dos casos da ilha principal de Singapura e das bacias hidrográficas dos rios Piracicaba, Capivari e Jundiaí, SP/MG

    OpenAIRE

    Mario Masaru Sakaguti Junior

    2016-01-01

    Singapura e as bacias hidrográficas dos rios Piracicaba, Capivari e Jundiaí (PCJ), Brasil, possuem como características comuns populações de cerca de 5,5 milhões de pessoas, altos índices de urbanização e industrialização e situações críticas de disponibilidade hídrica. O objetivo geral desta pesquisa é investigar e comparar a gestão, a governança e o uso das águas na ilha principal de Singapura e nas Bacias PCJ, a partir de dados obtidos por meio de trabalhos de campo, pesquisas bibliográfic...

  2. Susceptibilidade de Aedes aegypti aos inseticidas temephos e cipermetrina, Brasil Susceptibility of Aedes aegypti to temephos and cypermethrin insecticides, Brazil

    Directory of Open Access Journals (Sweden)

    Jonny E Duque Luna

    2004-12-01

    Full Text Available Realizaram-se bioensaios para detectar a susceptibilidade de Aedes aegypti aos inseticidas químicos, temefós e cipermetrina. Os resultados mostraram que esta espécie é suscetível a temefós e apresenta resistência a cipermetrinae.Bioassays were performed in order to detect the susceptibility of Aedes aegypti to the chemical insecticides temephos and cypermethrin. The results showed that this species is susceptible to temephos and presents resistance to cypermethrin.

  3. Voltage-gated sodium channel polymorphism and metabolic resistance in pyrethroid-resistant Aedes aegypti from Brazil.

    Science.gov (United States)

    Martins, Ademir Jesus; Lins, Rachel Mazzei Moura de Andrade; Linss, Jutta Gerlinde Birgitt; Peixoto, Alexandre Afranio; Valle, Denise

    2009-07-01

    The nature of pyrethroid resistance in Aedes aegypti Brazilian populations was investigated. Quantification of enzymes related to metabolic resistance in two distinct populations, located in the Northeast and Southeast regions, revealed increases in Glutathione-S-transferase (GST) and Esterase levels. Additionally, polymorphism was found in the IIS6 region of Ae. aegypti voltage-gated sodium channel (AaNa(V)), the pyrethroid target site. Sequences were classified in two haplotype groups, A and B, according to the size of the intron in that region. Rockefeller, a susceptible control lineage, contains only B sequences. In field populations, some A sequences present a substitution in the 1011 site (Ile/Met). When resistant and susceptible individuals were compared, the frequency of both A (with the Met mutation) and B sequences were slightly increased in resistant specimens. The involvement of the AaNa(V) polymorphism in pyrethroid resistance and the metabolic mechanisms that lead to potential cross-resistance between organophosphate and pyrethroids are discussed.

  4. Remanescentes populacionais nas ilhas do rio Paraná: aspectos sociais e econômicos = Population remnants in the islands of the Paraná river: social and economic aspects

    Directory of Open Access Journals (Sweden)

    Claudinéia Almeida da Silva

    2008-04-01

    Full Text Available A moderna acepção de ecologia compreende o ser humano e a sociedade como parte da natureza. No Brasil, esta concepção está longe da realidade, sobretudo quanto às populações tradicionais que são expulsas de seus ambientes naturais, para favorecer o poderpolítico de grupos econômicos hegemônicos. Muitas vezes, esta exclusão é justificada pela idéia de preservação do ambiente natural. Neste contexto, estudamos as relações de uma população de ilhéus residentes próximos ao rio Paraná, habitantes de uma Área de ProteçãoAmbiental (APA. Utilizamos questionários abertos para obter informações quanto a aspectos sociais e econômicos do grupo remanescente em cinco ilhas do rio Paraná, próximas das cidades de Porto Rico, Querência do Norte e São Pedro do Paraná. Resultadosobtidos em 2005 demonstraram que as estratégias de sobrevivência dos ilhéus estão voltadas a agricultura de subsistência e pesca. Entretanto, na Área de Proteção Ambiental das Ilhas e Várzeas do rio Paraná (APAIV, estes dois modos de sobrevivência local estão sendoinviabilizados pela fiscalização dos órgãos responsáveis pela execução da política ambiental brasileira. A baixa renda obtida pelos ilhéus em suas atividades, aliada as inúmeras restrições do uso da terra, contribui para a dizimação de sua cultura.The modern ecology meaning understands the people and the societyas part of the nature. In Brazil, that conception is still far away from our reality. Traditional populations are expelled of their habitat, to favor the political power of the groups economical. Usually, that exclusion is justified by the idea of preservation of the habitat and reduction of environmental impacts. We studied the relationships of a population of close resident islanders to the Paraná river, inhabitants of a Protected Area (APA. We utilizedquestionnaires to obtain information as for the social and economical aspects of the remaining group. Five

  5. Primer reporte de la mutación F1534C asociada con resistencia cruzada a DDT y piretroides en Aedes aegypti en Colombia

    Directory of Open Access Journals (Sweden)

    María Claudia Atencia

    2016-09-01

    Full Text Available Introducción. La principal estrategia para el control de Aedes aegypti, vector de los virus del dengue, del chikungunya y del zika, se basa en la utilización de insecticidas con el fin de disminuir su población. Sin embargo, su uso ha implicado que el insecto desarrolle resistencia a estos agentes químicos. Objetivo. Determinar la presencia de la mutación F1534C asociada con resistencia cruzada al DDT y los piretroides en mosquitos de la especie A. aegypti en Sincelejo, Colombia. Materiales y métodos. El estudio se desarrolló con nueve ejemplares de A. aegypti que mostraron resistencia a lambdacialotrina en bioensayos desarrollados por la Secretaría de Salud de Sucre. Se utilizó una reacción en cadena de la polimerasa (PCR semianidada siguiendo la metodología descrita por Harris, et al., para amplificar el exón 31 del gen para del canal de sodio dependiente de voltaje de A. aegypti. Los productos de la PCR se secuenciaron, editaron y analizaron con el programa MEGA 5. Resultados. En todos los mosquitos evaluados se detectó la presencia del alelo silvestre y mutante del exón 31. En la secuencia de nucleótidos del alelo mutante, se observó la sustitución de timina por guanina, la cual produce el cambio del codón UUC por UGC y conlleva el reemplazo del aminoácido fenilalanina por cisteína en el residuo 1534 de la proteína. Conclusión. Los nueve mosquitos analizados presentaron un genotipo heterocigoto para la mutación F1534C, cuyo efecto fenotípico es la resistencia al “derribo” (knock-down resistance, kdr con DDT y piretroides.

  6. Palinologia de espécies de Asteraceae de utilidade medicinal para a comunidade da Vila Dois Rios, Ilha Grande, Angra dos Reis, RJ, Brasil Pollen morpology of species of Asteraceae with medicinal utility for the community from Vila Dois Rios, Ilha Grande, Angra dos Reis, Rio de Janeiro State, Brazil

    Directory of Open Access Journals (Sweden)

    Marcelo Neto Galvão

    2009-03-01

    Full Text Available O trabalho trata da morfologia polínica e de descrições taxonômicas de 12 espécies de Asteraceae correspondentes em 12 diferentes gêneros, distribuídos em nove tribos. Todas as espécies estudadas são utilizadas como medicamentos pelos moradores da Vila Dois Rios, Ilha Grande, Angra dos Reis, Rio de Janeiro. Neste estudo objetivou-se caracterizar os tipos polínicos das espécies usadas por esta comunidade e fornecer chaves diagnósticas de caracteres macromorfológicos e micromorfológicos que auxiliem a identificação das mesmas. A identificação taxonômica e palinológica correta é pré-requisito para estudos que validem a eficácia e segurança terapêutica dessas espécies. Deve-se considerar, ainda, que a utilização desses conhecimentos é importante para a comunidade, como por exemplo, na melissopalinologia. Os grãos de pólen foram acetolisados, medidos, descritos e ilustrados sob microscopia de luz. Para observar detalhes da superfície e abertura, grãos de pólen não acetolisados foram analisados em microscópio eletrônico de varredura. Foram estudadas as características dos grãos de pólen como forma, tamanho, constituição da sexina e abertura. Os resultados mostraram que a morfologia polínica é bem definida entre as espécies estudadas, podendo ser usada para sua identificação e sustenta o caráter euripalinológico de Asteraceae.This study is a palynologycal characterization and taxonomic description of 12 species within 12 genera, distributed in nine tribes from Asteracaeae. All studied species are used as medicines by people from Vila Dois Rios and they occur in Ilha Grande. This work aimed to characterize the pollen grains of medicinal species of Asteraceae from Vila Dois Rios and provide diagnostic keys based on macromorphological and pollen characters to help their identification, since the correct taxonomic and palynological recognition of these species is necessary for further studies on therapeutic

  7. Parasites in stool samples in the environment of Ilha da Marambaia, Rio de Janeiro, Brazil: an approach in public health

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    Beatriz Coronato

    2012-04-01

    Full Text Available This research aimed to describe the frequency of parasites in stool samples in the environment of Ilha da Marambaia, Rio de Janeiro, Brazil. One hundred and five stool samples were collected and processed by the coproparasitological techniques ethyl acetate sedimentation and centrifuge-flotation using saturated sugar solution. Parasites were detected in 81.9% of the samples, hookworm being the most prevalent, followed by Trichuris vulpis. Ascaris sp. eggs were also found. A high level of evolutive forms of parasites with public health risk was found in stool samples of the environment studied. We propose that health education programs, allied to an improvement of human and animal health care, must be employed to reduce the environmental contamination.

  8. Radionuclide dispersion and hydrodynamics of Ilha Grande Bay (Angra dos Reis, RJ) simulated from hypothetical accidental releases of liquid wastes

    International Nuclear Information System (INIS)

    Simoes Filho, Fernando Lamego; Lapa, Celso Marcelo Franklin; Aguiar, Andre Silva; Soares, Abner Duarte

    2011-01-01

    This study has the aim to assess the impact of accidental release of radionuclides postulate from a nuclear power reactor through environmental modeling of aquatic resources. In order to do that it was used computational models of hydrodynamic circulation and transport for the simulation of tritium dispersion caused by an accidental release in Ilha Grande Bay from the site of the future third plant in two circulation scenarios. The main difference between the scenarios is based on the enhancement of dilution of the highest concentrations in the last one. This dilution enhancement resulting in decreasing concentrations was observed only during the first two weeks, when they ranged from 1 x 10 9 to 5 x 10 5 Bq/m³ close to the Itaorna beach spreading just to Sandri Island. After 180 days, the plume could not be detected anymore in the bay, because their activities would be lower than the minimum detectable value (< 11 kBq/m³). (author)

  9. FEAR BOUDARIES: RESISTENCE TO TOURISM PROJECTS AT ILHA DO MEDO (ISLE OF FEAR – MARANHÃO STATE, BRAZIL

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    Emilene Leite de Sousa

    2012-03-01

    Full Text Available The article analyses a project for communitarian tourism at Ilha do Medo, Maranhão State and the local‟s reaction to tourists. It also analyses dialogues between natives people and tourism planners in the place. Local´s reaction to the attempt of turning the island into a new attraction at San Luis indicates their concern in preserving their way of life. To do so they use fear as a strategy to keep “invaders” at a distance. The analysis made possible to reflect on relationships between tourists and native people, understand the social network woven among tourism planners, natives and ethnographers in the field, as well as learning the flux at the boundaries of fear.

  10. Spatial distribution of potential and positive Aedes aegypti breeding sites

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    Daniel Elías Cuartas

    2017-03-01

    Conclusions: The spatial relationship between positive and potential A. aegypti breeding sites both indoors and outdoors is dynamic and highly sensitive to the characteristics of each territory. Knowing how positive and potential breeding sites are distributed contributes to the prioritization of resources and actions in vector control programs.

  11. A review on symmetries for certain Aedes aegypti models

    Science.gov (United States)

    Freire, Igor Leite; Torrisi, Mariano

    2015-04-01

    We summarize our results related with mathematical modeling of Aedes aegypti and its Lie symmetries. Moreover, some explicit, group-invariant solutions are also shown. Weak equivalence transformations of more general reaction diffusion systems are also considered. New classes of solutions are obtained.

  12. Degree of threat to the biological diversity in the Ilha Grande State Park (RJ) and guidelines for conservation.

    Science.gov (United States)

    Alho, C J R; Schneider, M; Vasconcellos, L A

    2002-08-01

    The State Park of Ilha Grande is only a part (5,594 hectares) of the entire island (19,300 hectares) which is located off the south coast of Rio de Janeiro state, between the cities of Mangaratiba and Angra dos Reis. Approximately half of the Park area (47%) is covered by dense Atlantic forest. The secondary forest growth is in a process of ecological succession close to attaining maturity (43%) and the remaining part (10%) is composed of human-altered areas (1%), rocky outcrops with herbaceous vegetation (7%), mangroves and beaches (2%). The fauna is well represented but already shows signs of degradation with introduced species. The analysis of the degree of threat has shown that the dense forest habitat has a relatively stable status of conservation while the secondary forest, the mangrove and the herbaceous vegetation on rocky outcrops (and their fauna) are categorized as vulnerable. The area altered by human occupation is considered threatened. Since the coastal area where Ilha Grande is located is well known for its beautiful scenery (known as the green coast, because of the contrast between the ocean and the Atlantic forest covering the Serra do Mar mountain chain). There is a strong possibility for tourism to become the means in which to achieve economic sustainability for conservation. Contradictorily, tourism is also the major threat to local biodiversity and its landscape units. Because tourism is not organized and controlled, during high season the numbers grow above local capacity, giving rise to a proliferation of hotels, guesthouses and camping grounds. The resulting untreated open sewage, random garbage disposal and other harmful activities form the major threats to biodiversity.

  13. Contraceptive use and associated factors among adolescents on Santiago Island - Cape Verde - Western Africa Uso de métodos anticoncepcionais e fatores associados em adolescentes da Ilha de Santiago - Cabo Verde - África Ocidental

    Directory of Open Access Journals (Sweden)

    Carlos Mendes Tavares

    2010-06-01

    Full Text Available OBJECTIVE: to estimate the prevalence of contraceptive use and associated factors among adolescents attending public schools on Santiago Island, Cape Verde. METHODS: a cross-sectional study was carried out with 368 sexually active adolescents aged 13-17years attending eight public elementary and high schools, randomly selected, on Santiago Island, Cape Verde, between January and March 2007. Poisson regression with robust variance was used for the multiple analysis of risk factors, at a 5% level of significance. RESULTS: among 368 adolescents, 69.3% reported having used a contraceptive method during the last act of sexual intercourse. The most frequently used method were condom (94.9% and pill (26.4%. Factors significantly and positively associated with contraceptive use were: living in the capital (PR=1.23; CI95%: 1.07; 1.39; having dated and had sexual intercourse (PR =1.53; CI95%: 1.14;2.06; and having more than nine years of schooling (PR=1.19; CI95%: 1.02; 1.38. CONCLUSIONS: more educated adolescents who studied in Praia (the capital city and were dating at the time of the study were more likely to use contraception. The high prevalence of condom use and the association between contraception use and years of schooling among adolescents may indicate that sexual and reproductive health policies have produced positive outcomes that may account for the decrease in HIV infection.OBJETIVO: estimar a prevalência do uso de métodos anticoncepcionais (MAC e fatores associados entre adolescentes de escola pública na ilha de Santiago em Cabo Verde. MÉTODOS: estudo transversal, realizado entre janeiro e março de 2007, com amostra aleatória de 368 adolescentes de 13 a 17 anos de idade, com vida sexual ativa, provenientes de oito escolas secundárias públicas da ilha de Santiago. Para análise múltipla dos fatores de risco foi utilizada a regressão de Poisson ajustada pela estimativa robusta de variância considerando-se nível de signific

  14. EFFECT OF STORAGE TEMPERATURE PERCENTAGE OF EGG HATCHING OF AEDES AEGYPTI IN LABORATORY=PENGARUH SUHU PENYIMPANAN TERHADAP PRESENTASE TETAS TELUR Aedes aegypti DI LABORATORIUM

    Directory of Open Access Journals (Sweden)

    Riyani Setiyaningsih

    2015-03-01

    Full Text Available EnglishABSTRACTAedes aegypti is the vector of Dengue Hemorrhagic Fever (DHF in Indonesia. Ae. aegypti has a high reproductive capacity, one female mosquitoes can lay 100-150 eggs. Eggs of Ae. aegypti can survive on dry temperatures within a few months, thus increasing the chances of transmission of dengue virus The aim of the study was to determine the effect of temperature on egg hatching percentage of Ae. aegypti. Eggs Ae. aegypti colonization in laboratory results are stored at room temperature and refrigerator temperature. Observations percentage of eggs hatching was observed at month zero, one, two, third, fourth, fifth, and sixth. The results of the study until the sixth month percentage of hatching eggs at room temperature was 63,17, 59,26, 24,33, 13,62, 10, and 0%. While storage on egg hatching refrigerator not occur in the first to sixth.INDONESIANAedes aegypti merupakan vektor Demam Berdarah Dengue (DBD di Indonesia. Ae. aegypti memiliki kemampuan reproduksi yang tinggi, satu ekor nyamuk betina dapat bertelur 100-150 butir telur. Telur Ae. aegypti mampu bertahan hidup pada suhu kering dalam beberapa bulan sehingga memperbesar peluang terjadinnya proses penularan virus DBD. Tujuan penelitian adalah mengetahui pengaruh suhu dan lama penyimpanan terhadap presentase penetasan telur Ae. aegypti. Telur Ae. aegypti hasil kolonisasi di laboratorium disimpan pada suhu ruang dan suhu refrigerator. Pengamatan presentase penetasan telur diamati pada bulan ke nol, kesatu, kedua, ketiga, keempat, kelima, dan keenam. Hasil penelitian pada bulan bertama sampai keenam presentase penetasan telur pada suhu ruang adalah 63,17, 59,26, 24,33, 13,62, 10, dan 0%. Sedangkan penyimpanan pada suhu kukas tidak terjadi penetasan telur pada bulan pertama sampai keenam.

  15. Schinus terebinthifolius Leaf Extract Causes Midgut Damage, Interfering with Survival and Development of Aedes aegypti Larvae.

    Science.gov (United States)

    Procópio, Thamara Figueiredo; Fernandes, Kenner Morais; Pontual, Emmanuel Viana; Ximenes, Rafael Matos; de Oliveira, Aline Rafaella Cardoso; Souza, Carolina de Santana; Melo, Ana Maria Mendonça de Albuquerque; Navarro, Daniela Maria do Amaral Ferraz; Paiva, Patrícia Maria Guedes; Martins, Gustavo Ferreira; Napoleão, Thiago Henrique

    2015-01-01

    In this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4), as well as for toxic effect on Artemia salina. Leaf extract was obtained using 0.15 M NaCl and evaluated for phytochemical composition and lectin activity. Early L4 larvae were incubated with the extract (0.3-1.35%, w/v) for 8 days, in presence or absence of food. Polymeric proanthocyanidins, hydrolysable tannins, heterosid and aglycone flavonoids, cinnamic acid derivatives, traces of steroids, and lectin activity were detected in the extract, which killed the larvae at an LC50 of 0.62% (unfed larvae) and 1.03% (fed larvae). Further, the larvae incubated with the extract reacted by eliminating the gut content. No larvae reached the pupal stage in treatments at concentrations between 0.5% and 1.35%, while in the control (fed larvae), 61.7% of individuals emerged as adults. The extract (1.0%) promoted intense disorganization of larval midgut epithelium, including deformation and hypertrophy of cells, disruption of microvilli, and vacuolization of cytoplasms, affecting digestive, enteroendocrine, regenerative, and proliferating cells. In addition, cells with fragmented DNA were observed. Separation of extract components by solid phase extraction revealed that cinnamic acid derivatives and flavonoids are involved in larvicidal effect of the extract, being the first most efficient in a short time after larvae treatment. The lectin present in the extract was isolated, but did not show deleterious effects on larvae. The extract and cinnamic acid derivatives were toxic to A. salina nauplii, while the flavonoids showed low toxicity. S. terebinthifolius leaf extract caused damage to the midgut of A. aegypti larvae, interfering with survival and development. The larvicidal effect of the extract can be attributed to cinnamic acid derivatives and flavonoids. The data obtained using A. salina indicates that caution

  16. Schinus terebinthifolius Leaf Extract Causes Midgut Damage, Interfering with Survival and Development of Aedes aegypti Larvae.

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    Thamara Figueiredo Procópio

    Full Text Available In this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4, as well as for toxic effect on Artemia salina. Leaf extract was obtained using 0.15 M NaCl and evaluated for phytochemical composition and lectin activity. Early L4 larvae were incubated with the extract (0.3-1.35%, w/v for 8 days, in presence or absence of food. Polymeric proanthocyanidins, hydrolysable tannins, heterosid and aglycone flavonoids, cinnamic acid derivatives, traces of steroids, and lectin activity were detected in the extract, which killed the larvae at an LC50 of 0.62% (unfed larvae and 1.03% (fed larvae. Further, the larvae incubated with the extract reacted by eliminating the gut content. No larvae reached the pupal stage in treatments at concentrations between 0.5% and 1.35%, while in the control (fed larvae, 61.7% of individuals emerged as adults. The extract (1.0% promoted intense disorganization of larval midgut epithelium, including deformation and hypertrophy of cells, disruption of microvilli, and vacuolization of cytoplasms, affecting digestive, enteroendocrine, regenerative, and proliferating cells. In addition, cells with fragmented DNA were observed. Separation of extract components by solid phase extraction revealed that cinnamic acid derivatives and flavonoids are involved in larvicidal effect of the extract, being the first most efficient in a short time after larvae treatment. The lectin present in the extract was isolated, but did not show deleterious effects on larvae. The extract and cinnamic acid derivatives were toxic to A. salina nauplii, while the flavonoids showed low toxicity. S. terebinthifolius leaf extract caused damage to the midgut of A. aegypti larvae, interfering with survival and development. The larvicidal effect of the extract can be attributed to cinnamic acid derivatives and flavonoids. The data obtained using A. salina indicates

  17. Patterns of geographic expansion of Aedes aegypti in the Peruvian Amazon.

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    Sarah Anne Guagliardo

    2014-08-01

    Full Text Available In the Peruvian Amazon, the dengue vector Aedes aegypti is abundant in large urban centers such as Iquitos. In recent years, it has also been found in a number of neighboring rural communities with similar climatic and socioeconomic conditions. To better understand Ae. aegypti spread, we compared characteristics of communities, houses, and containers in infested and uninfested communities.We conducted pupal-demographic surveys and deployed ovitraps in 34 communities surrounding the city of Iquitos. Communities surveyed were located along two transects: the Amazon River and a 95 km highway. We calculated entomological indices, mapped Ae. aegypti presence, and developed univariable and multivariable logistic regression models to predict Ae. aegypti presence at the community, household, or container level.Large communities closer to Iquitos were more likely to be infested with Ae. aegypti. Within infested communities, houses with Ae. aegypti had more passively-filled containers and were more often infested with other mosquito genera than houses without Ae. aegypti. For containers, large water tanks/drums and containers with solar exposure were more likely to be infested with Ae. aegypti. Maps of Ae. aegypti presence revealed a linear pattern of infestation along the highway, and a scattered pattern along the Amazon River. We also identified the geographical limit of Ae. aegypti expansion along the highway at 19.3 km south of Iquitos.In the Peruvian Amazon, Ae. aegypti geographic spread is driven by human transportation networks along rivers and highways. Our results suggest that urban development and oviposition site availability drive Ae. aegypti colonization along roads. Along rivers, boat traffic is likely to drive long-distance dispersal via unintentional transport of mosquitoes on boats.

  18. Oral infection of Aedes aegypti with yellow fever virus: geographic variation and genetic considerations.

    Science.gov (United States)

    Tabachnick, W J; Wallis, G P; Aitken, T H; Miller, B R; Amato, G D; Lorenz, L; Powell, J R; Beaty, B J

    1985-11-01

    Twenty-eight populations representing a worldwide distribution of Aedes aegypti were tested for their ability to become orally infected with yellow fever virus (YFV). Populations had been analyzed for genetic variations at 11 isozyme loci and assigned to one of 8 genetic geographic groups of Ae. aegypti. Infection rates suggest that populations showing isozyme genetic relatedness also demonstrate similarity to oral infection rates with YFV. The findings support the hypothesis that genetic variation exists for oral susceptibility to YFV in Ae. aegypti.

  19. The Maxillary Palp of Aedes aegypti, a Model of Multisensory Integration

    Science.gov (United States)

    2014-01-01

    The maxillary palp of Aedes aegypti, a model of multisensory integration Jonathan D. Bohbot, Jackson T. Sparks, Joseph C. Dickens* United States...24 February 2014 Keywords: Aedes aegypti Olfaction Mosquito Maxillary palp Thermosensation Mechanosensation a b s t r a c t Female yellow-fever...mosquitoes, Aedes aegypti, are obligate blood-feeders and vectors of the pathogens that cause dengue fever, yellow fever and Chikungunya. This feeding

  20. Toxicity of Thiophenes from Echinops transiliensis (Asteraceae) against Aedes aegypti (Diptera: Culicidae) Larvae

    Science.gov (United States)

    2014-01-01

    Toxicity of Thiophenes from Echinops transiliensis (Asteraceae) against Aedes aegypti (Diptera: Culicidae) Larvae by Hiroshi Nakano*a)b)c), Abbas...larvicides against Aedes aegypti. Structural differences among compounds 3, 5, and 8 consisted in differing AcO and OH groups attached to C(3’’) and C(4...serious human diseases including malaria, Japanese encephalitis, yellow fever, dengue, and filariasis. The urban-adapted Aedes aegypti mosquito has become

  1. Pengaruh Frekuensi Penghisapan Darah Terhadap Perkembangan, Reproduksi,vertilitas Dan Rasio Sex Aedes Aegypti

    OpenAIRE

    Setiyaningsih, Riyani; Agustini, Maria

    2012-01-01

    Aedes aegypti is a vector of Dengue hemorrhagic fever in Indonesia. Aedesaegypti has a high reproduction ability. Each individual can produce 50-100 eggs. Which80% of them are fertile. The mosquito is multiple biting (which means each individualsucks blood several time). Based on that background, this research was aimed torecognize the frequency of blood sucking to development, reproduction, fertility, and sexratio of Ae. aegypti. Thirty Ae. aegypti mosquito were put into the plastic cupsindi...

  2. Estruturas das comunidades de peixes recifais em ilhas oceânicas do atlântico e pacífico oriental

    OpenAIRE

    Quimbayo Agreda, Juan Pablo

    2013-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas. Programa de Pós-Graduação em Ecologia, Florianópolis, 2013 As ilhas oceânicas, apesar de representarem uma pequena fração dos ambientes marinhos do planeta, tem contribuido para o entendimento de processos ecológicos e evolutivos. No entanto,

  3. Avaliação do impacto na infestação por Aedes aegypti em tanques de cimento do município de Canindé, Ceará, Brasil, após a utilização do peixe Betta splendens como alternativa de controle biológico Evaluation of the impact on Aedes aegypti infestation in cement tanks of the municipal district of Canindé, Ceará, Brazil after using the Betta splendens fish as an alternative biological control

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    Luciano de Góes Cavalcanti Pamplona

    2004-10-01

    Full Text Available Canindé apresenta uma população de 71.235 habitantes. Em abril de 2001 iniciou a utilização de peixes larvófagos em tanques de cimento, localizados ao nível do solo, como forma de controle biológico para larvas de Aedes aegypti. Durante a visita do agente, ao invés de se tratar os tanques com larvicida, colocou-se um espécime do peixe Betta splendens por depósito. O presente trabalho teve como objetivo avaliar os resultados desta intervenção. Com os levantamentos do número de imóveis e depósitos existentes, estimou-se este número mês a mês determinando então o número de depósitos existentes por imóvel. Com esta estimativa e o número de imóveis visitados mensalmente analisou-se a infestação deste tipo de depósito. Em janeiro de 2001, 70,4% dos tanques examinados apresentavam larvas; e apenas 7,4% em janeiro de 2002. Em dezembro de 2002 este índice caiu para 0,2%. Demonstrou-se com clareza a capacidade do Betta splendens como agente de controle biológico, em tanques de cimento, reduzindo 320 vezes a infestação deste tipo de recipiente de grande volume.Canindé has a population of 71,235 inhabitants. In April 2001, the city started using larvivorous fish in cement water tanks, as a means of biological control of Aedes aegypti larvae. During house-to-house visits by health agents, instead of treating the water tank with larvicide, a Betta splendens fish was introduced into each tank. The number of houses and the number of tanks was estimated by monthly surveys. Then, the number of tanks per house was determined. Taking into account this estimated number and the number of houses visited, the infestation level for each kind of deposit was analyzed. In January 2001, 70.4% of the water tanks presented mosquitoes. Following the intervention, in January 2002 only 7.4% were positive and by December 2002 the rate had dropped to 0.2%. The efficacy of Betta splendens as a biological control agent in cement water tanks was

  4. CARACTERIZAÇÃO GEOMORFOLÓGICA DAS ÁREAS LIVRES DE GELO EM RESPOSTA DA TENDÊNCIA DE RETRAÇÃO DA GELEIRA POLAR CLUB, PENÍNSULA POTTER, ILHA REI GEORGE, ANTÁRTICA

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    Betânia Bonada

    2018-01-01

    Full Text Available Este trabalho objetiva investigar a dinâmica geomorfológica glacial das áreas livres de gelo na Península Potter, Ilha Rei George, Antártica, em resposta à recente retração da geleira Polar Club. O mapeamento geomorfológico e a interpretação da evolução dos sistemas lacustres entre 2006 e 2011 das áreas livres de gelo na Península Potter foi realizado através da interpretação visual em uma imagem Quickbird (RGB432, em imagens COSMO-SkyMed polarizações VV e HH em modo spotlight processadas com filtros espaciais e perfis topográficos. Também foram considerados os aspectos morfométricos da península, interpretados através da geração de mapas de hipsometria, declividade, curvas de nível e sombreamento. A variação frontal da geleira Polar Club entre os anos de 1981 a 2015 foi obtida pela análise temporal de imagens Landsat. O mapeamento geomorfológico da Península Potter evidenciou os processos geomorfológicos proglaciais e o padrão de disposição espacial das feições lineares marginais ao gelo, como cordões morâinicos e feições glaciofluviais (relacionadas ao aporte da fusão sazonal da neve e do gelo, bem como terraços marinhos, ravinas e afloramentos rochosos. O modelo de evolução da variação frontal da geleira Polar Club indica que condição de último avanço glacial registrado pela geomorfologia proglacial pode estar relacionado à Pequena Idade do Gelo e que houve um contínuo processo de retração desde então. Entre 1981-2015 evidenciou-se a redução de área de 2,95km² para a geleira Polar Club (perda de área total de 9,4%. Este processo pode estar relacionado com a tendência de aumento das temperaturas médias do ar, a tendência de aumento de dias com precipitação líquida no verão e o número de dias em que a temperatura média ultrapassou os 0°C na região nas últimas décadas. O recuo da geleira Polar Club é pouco expressivo quando comparado a outras geleiras da Ilha Rei George

  5. Comparative efficacy of existing surveillance tools for Aedes aegypti in Western Kenya.

    Science.gov (United States)

    Yalwala, Sancto; Clark, Jeffrey; Oullo, David; Ngonga, Daniel; Abuom, David; Wanja, Elizabeth; Bast, Joshua

    2015-12-01

    All traditional surveillance techniques for Aedes aegypti have been developed for the cosmopolitan domestic subspecies Ae. aegypti aegypti, and not the sylvatic subspecies, Ae. aegypti formosus. The predominant form in Western Kenya is Ae. aegypti formosus that is rarely associated with human habitations but is linked to transmission of sylvatic dengue virus strains. We compared five surveillance methods for their effectiveness in sampling Ae. aegypti formosus with the goal of determining a sustainable surveillance strategy in Kenya. The methods included larval and pupal surveys, oviposition trapping, BG-Sentinel trapping, resting boxes, and backpack aspirations. Larval and pupal surveys collected the highest number of Ae. aegypti formosus (51.3%), followed by oviposition traps (45.7%), BG-Sentinel traps (3.0%), and zero collected with either backpack aspiration or resting box collections. No Ae. aegypti formosus larvae or pupae were found indoors. The results indicate that oviposition traps and outdoor larval and pupal surveys were better surveillance methods for Ae. aegypti formosus in Western Kenya. © 2015 The Society for Vector Ecology.

  6. Aktivitas Beberapa Atraktan Pada Perangkap Telur Berperekat Terhadap Aedes aegypti

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    Milana Salim

    2017-02-01

    Full Text Available AbstractControl of Aedes aegypti mosquito as dengue haemorrhagic fever/DHF vector can be conducted using the ovitrap modified into a sticky ovitrap. The addition of attractant substances to the ovitrap can attract more mosquitoes comes to the trap, and prevent mosquitoes laying eggs in other places. The aim of this research was to compare hay infusion water and larva rearing water as attractant which combined with sugar-apple (Annona squamosa seed extract by counting the mosquitoes and eggs trapped. This research used six types medium: hay infusion water, larva rearing water, hay infusion water + sugar-apple seed extract, larva rearing water + sugar-apple seed extract, aquadest + sugar-apple seed extract, and aquadest only as a control. Sample used were 25 gravid female of Ae. aegypti mosquitoes with five replications. Mosquitoes and eggs which trapped were counted. This research showed that the number of mosquito trapped and eggs hatched more found in sticky ovitrap with hay infusion water. Statistic analyzed by ANOVA showed that there is no significant difference towards number off mosquito trapped in sticky ovitrap (p>0,05 whereas the medium material has significant difference towards number off egg hatched than others (p<0,05.Keywords: Sticky ovitrap, attractant, Aedes aegypti AbstrakPengendalian nyamuk Aedes aegypti sebagai vektor demam berdarah dengue/DBD dapat dilakukan menggunakan ovitrap yang dimodifikasi dengan perekat menjadi sticky ovitrap. Penambahan atraktan pada ovitrap dapat menarik lebih banyak nyamuk datang ke perangkap yang dipasang dan mencegah nyamuk bertelur di tempat lain. Tujuan penelitian ini adalah untuk mengetahui aktivitas atraktan air rendaman jerami dan air bekas kolonisasi yang dikombinasikan dengan ekstrak biji srikaya pada sticky ovitrap terhadap jumlah nyamuk dan telur yang ditemukan. Enam jenis media uji digunakan dalam penelitian ini yaitu air rendaman jerami, air bekas kolonisasi nyamuk, air rendaman jerami

  7. Patrimônio cultural do Parque Estadual da Ilha do Cardoso, Estado de São Paulo. Cultural heritage of Ilha do Cardoso State Park, state of São Paulo.

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    Lucilia KOTEZ

    2010-06-01

    Full Text Available No presente trabalho, realizou-se um levantamento dos bens culturais do Parque Estadual da Ilha Cardoso – PEIC, Unidade de Conservação localizada no litoral sul do Estado de São Paulo, contribuindo para o estabelecimento de um conjunto de ações efetivas de valorização e proteção do patrimônio cultural do Parque. Um total de 49 bens culturais foi inventariado, sendo 28 sítios arqueológicos e 21 equipamentos tradicionais (artefatos relacionados com a cultura caiçara. Os sítios arqueológicos são compostos por 14 sítios pré-históricos (sambaquis e 14 históricos – um marco do Tratado de Tordesilhas, um caminho histórico, ambos relacionados com o primeiro período de ocupação da ilha (entre 1554 a 1850, bem como 12 vestígios de estruturas arquitetônicas relacionadas como final do século XIX e início do século XX. Os equipamentos tradicionais estão relacionados à ocupação recente e consistem em 17 cercos (método típico da pesca, e em quatro casas de farinha (indústria doméstica de farinha de mandioca. O mapeamento proporciona um panorama sobre a riqueza do patrimônio cultural do PEIC, possibilitando apontar proposições para ações e atividades fundamentais para uma eficiente gestão desses bens.In the present work it was carried out an inventory of cultural assets of Ilha do Cardoso State Park – PEIC, a Conservation Unit located on the southern coast of the State of São Paulo, contributing to the establishment of a set of effective actions for recovery and protection of cultural heritage of the Park. A total of forty-nine cultural items were surveyed: twenty-eight archaeological sites and twenty-one traditional equipments(artifacts related to the caiçara culture. The archaeological sites consist of fourteen prehistoric sites (shell middens and fourteen historic as a landmark of the Treaty of Tordesillas, a historic trail, both related to the first period of occupation of the island (between 1554 to 1850

  8. Truck-mounted Area-wide Application of Pyriproxyfen Targeting Aedes aegypti and Aedes albopictus in Northeast Florida

    Science.gov (United States)

    2014-12-01

    TRUCK-MOUNTED AREA-WIDE APPLICATION OF PYRIPROXYFEN TARGETING AEDES AEGYPTI AND AEDES ALBOPICTUS IN NORTHEAST FLORIDA1 CARL W. DOUD,2,3 ANTHONY M...truck-mounted ultra-low volume applications of pyriproxyfen against Aedes aegypti larvae in artificial water containers and wild adult Ae. albopictus...larval control, Aedes aegypti, Aedes albopictus INTRODUCTION Aedes albopictus (Skuse) (Asian tiger mosquito) and Ae. aegypti (L.) (yellow fever

  9. Sodium Channel Mutations and Pyrethroid Resistance in Aedes aegypti

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    Yuzhe Du

    2016-10-01

    Full Text Available Pyrethroid insecticides are widely used to control insect pests and human disease vectors. Voltage-gated sodium channels are the primary targets of pyrethroid insecticides. Mutations in the sodium channel have been shown to be responsible for pyrethroid resistance, known as knockdown resistance (kdr, in various insects including mosquitoes. In Aedes aegypti mosquitoes, the principal urban vectors of dengue, zika, and yellow fever viruses, multiple single nucleotide polymorphisms in the sodium channel gene have been found in pyrethroid-resistant populations and some of them have been functionally confirmed to be responsible for kdr in an in vitro expression system, Xenopus oocytes. This mini-review aims to provide an update on the identification and functional characterization of pyrethroid resistance-associated sodium channel mutations from Aedes aegypti. The collection of kdr mutations not only helped us develop molecular markers for resistance monitoring, but also provided valuable information for computational molecular modeling of pyrethroid receptor sites on the sodium channel.

  10. Targeted genome editing in Aedes aegypti using TALENs.

    Science.gov (United States)

    Aryan, Azadeh; Myles, Kevin M; Adelman, Zach N

    2014-08-15

    The Culicine mosquito, Aedes aegypti, is both a major vector of arthropod-borne viruses (arboviruses) and a genetic model organism for arbovirus transmission. TALE nucleases (TALENs), a group of artificial enzymes capable of generating site-specific DNA lesions, consist of a non-specific FokI endonuclease cleavage domain fused to an engineered DNA binding domain specific to a target site. While TALENs have become an important tool for targeted gene disruption in a variety of organisms, application to the mosquito genome is a new approach. We recently described the use of TALENs to perform heritable genetic disruptions in A. aegypti. Here, we provide detailed methods that will allow other research laboratories to capitalize on the potential of this technology for understanding mosquito gene function. We describe target site selection, transient embryo-based assays to rapidly assess TALEN activity, embryonic microinjection and downstream screening steps to identify target site mutations. Copyright © 2014 Elsevier Inc. All rights reserved.

  11. Identification of germline transcriptional regulatory elements in Aedes aegypti

    Science.gov (United States)

    Akbari, Omar S.; Papathanos, Philippos A.; Sandler, Jeremy E.; Kennedy, Katie; Hay, Bruce A.

    2014-02-01

    The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmitting mosquitoes with individuals refractory to disease transmission, or bringing about population suppression. Several of these systems, including Medea, UDMEL, and site-specific nucleases, which can be used to drive genes into populations or bring about population suppression, utilize transcriptional regulatory elements that drive germline-specific expression. Here we report the identification of multiple regulatory elements able to drive gene expression specifically in the female germline, or in the male and female germline, in the mosquito Aedes aegypti. These elements can also be used as tools with which to probe the roles of specific genes in germline function and in the early embryo, through overexpression or RNA interference.

  12. Variation in Aedes aegypti Mosquito Competence for Zika Virus Transmission.

    Science.gov (United States)

    Roundy, Christopher M; Azar, Sasha R; Rossi, Shannan L; Huang, Jing H; Leal, Grace; Yun, Ruimei; Fernandez-Salas, Ildefonso; Vitek, Christopher J; Paploski, Igor A D; Kitron, Uriel; Ribeiro, Guilherme S; Hanley, Kathryn A; Weaver, Scott C; Vasilakis, Nikos

    2017-04-01

    To test whether Zika virus has adapted for more efficient transmission by Aedes aegypti mosquitoes, leading to recent urban outbreaks, we fed mosquitoes from Brazil, the Dominican Republic, and the United States artificial blood meals containing 1 of 3 Zika virus strains (Senegal, Cambodia, Mexico) and monitored infection, dissemination, and virus in saliva. Contrary to our hypothesis, Cambodia and Mexica strains were less infectious than the Senegal strain. Only mosquitoes from the Dominican Republic transmitted the Cambodia and Mexica strains. However, blood meals from viremic mice were more infectious than artificial blood meals of comparable doses; the Cambodia strain was not transmitted by mosquitoes from Brazil after artificial blood meals, whereas 61% transmission occurred after a murine blood meal (saliva titers up to 4 log 10 infectious units/collection). Although regional origins of vector populations and virus strain influence transmission efficiency, Ae. aegypti mosquitoes appear to be competent vectors of Zika virus in several regions of the Americas.

  13. Multiple introductions of the dengue vector, Aedes aegypti, into California.

    Science.gov (United States)

    Pless, Evlyn; Gloria-Soria, Andrea; Evans, Benjamin R; Kramer, Vicki; Bolling, Bethany G; Tabachnick, Walter J; Powell, Jeffrey R

    2017-08-01

    The yellow fever mosquito Aedes aegypti inhabits much of the tropical and subtropical world and is a primary vector of dengue, Zika, and chikungunya viruses. Breeding populations of A. aegypti were first reported in California (CA) in 2013. Initial genetic analyses using 12 microsatellites on collections from Northern CA in 2013 indicated the South Central US region as the likely source of the introduction. We expanded genetic analyses of CA A. aegypti by: (a) examining additional Northern CA samples and including samples from Southern CA, (b) including more southern US populations for comparison, and (c) genotyping a subset of samples at 15,698 SNPs. Major results are: (1) Northern and Southern CA populations are distinct. (2) Northern populations are more genetically diverse than Southern CA populations. (3) Northern and Southern CA groups were likely founded by two independent introductions which came from the South Central US and Southwest US/northern Mexico regions respectively. (4) Our genetic data suggest that the founding events giving rise to the Northern CA and Southern CA populations likely occurred before the populations were first recognized in 2013 and 2014, respectively. (5) A Northern CA population analyzed at multiple time-points (two years apart) is genetically stable, consistent with permanent in situ breeding. These results expand previous work on the origin of California A. aegypti with the novel finding that this species entered California on multiple occasions, likely some years before its initial detection. This work has implications for mosquito surveillance and vector control activities not only in California but also in other regions where the distribution of this invasive mosquito is expanding.

  14. Intraspecific Competition and Population Dynamics of Aedes aegypti

    Science.gov (United States)

    Paixão, C. A.; Charret, I. C.; Lima, R. R.

    2012-04-01

    We report computational simulations for the evolution of the population of the dengue vector, Aedes aegypti mosquitoes. The results suggest that controlling the mosquito population, on the basis of intraspecific competition at the larval stage, can be an efficient mechanism for controlling the spread of the epidemic. The results also show the presence of a kind of genetic evolution in vector population, which results mainly in increasing the average lifespan of individuals in adulthood.

  15. Similarity solutions for systems arising from an Aedes aegypti model

    Science.gov (United States)

    Freire, Igor Leite; Torrisi, Mariano

    2014-04-01

    In a recent paper a new model for the Aedes aegypti mosquito dispersal dynamics was proposed and its Lie point symmetries were investigated. According to the carried group classification, the maximal symmetry Lie algebra of the nonlinear cases is reached whenever the advection term vanishes. In this work we analyze the family of systems obtained when the wind effects on the proposed model are neglected. Wide new classes of solutions to the systems under consideration are obtained.

  16. Multiple introductions of the dengue vector, Aedes aegypti, into California.

    Directory of Open Access Journals (Sweden)

    Evlyn Pless

    2017-08-01

    Full Text Available The yellow fever mosquito Aedes aegypti inhabits much of the tropical and subtropical world and is a primary vector of dengue, Zika, and chikungunya viruses. Breeding populations of A. aegypti were first reported in California (CA in 2013. Initial genetic analyses using 12 microsatellites on collections from Northern CA in 2013 indicated the South Central US region as the likely source of the introduction. We expanded genetic analyses of CA A. aegypti by: (a examining additional Northern CA samples and including samples from Southern CA, (b including more southern US populations for comparison, and (c genotyping a subset of samples at 15,698 SNPs. Major results are: (1 Northern and Southern CA populations are distinct. (2 Northern populations are more genetically diverse than Southern CA populations. (3 Northern and Southern CA groups were likely founded by two independent introductions which came from the South Central US and Southwest US/northern Mexico regions respectively. (4 Our genetic data suggest that the founding events giving rise to the Northern CA and Southern CA populations likely occurred before the populations were first recognized in 2013 and 2014, respectively. (5 A Northern CA population analyzed at multiple time-points (two years apart is genetically stable, consistent with permanent in situ breeding. These results expand previous work on the origin of California A. aegypti with the novel finding that this species entered California on multiple occasions, likely some years before its initial detection. This work has implications for mosquito surveillance and vector control activities not only in California but also in other regions where the distribution of this invasive mosquito is expanding.

  17. Insights into the transcriptome of oenocytes from Aedes aegypti pupae

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    Gustavo Ferreira Martins

    2011-05-01

    Full Text Available Oenocytes are ectodermic cells present in the fat body of several insect species and these cells are considered to be analogous to the mammalian liver, based on their role in lipid storage, metabolism and secretion. Although oenocytes were identified over a century ago, little is known about their messenger RNA expression profiles. In this study, we investigated the transcriptome of Aedes aegypti oenocytes. We constructed a cDNA library from Ae. aegypti MOYO-R strain oenocytes collected from pupae and randomly sequenced 687 clones. After sequences editing and assembly, 326 high-quality contigs were generated. The most abundant transcripts identified corresponded to the cytochrome P450 superfamily, whose members have roles primarily related to detoxification and lipid metabolism. In addition, we identified 18 other transcripts with putative functions associated with lipid metabolism. One such transcript, a fatty acid synthase, is highly represented in the cDNA library of oenocytes. Moreover, oenocytes expressed several immunity-related genes and the majority of these genes were lysozymes. The transcriptional profile suggests that oenocytes play diverse roles, such as detoxification and lipid metabolism, and increase our understanding of the importance of oenocytes in Ae. aegypti homeostasis and immune competence.

  18. Germline excision of transgenes in Aedes aegypti by homing endonucleases.

    Science.gov (United States)

    Aryan, Azadeh; Anderson, Michelle A E; Myles, Kevin M; Adelman, Zach N

    2013-01-01

    Aedes (Ae.) aegypti is the primary vector for dengue viruses (serotypes1-4) and chikungunya virus. Homing endonucleases (HEs) are ancient selfish elements that catalyze double-stranded DNA breaks (DSB) in a highly specific manner. In this report, we show that the HEs Y2-I-AniI, I-CreI and I-SceI are all capable of catalyzing the excision of genomic segments from the Ae. aegypti genome in a heritable manner. Y2-I-AniI demonstrated the highest efficiency at two independent genomic targets, with 20-40% of Y2-I-AniI-treated individuals producing offspring that had lost the target transgene. HE-induced DSBs were found to be repaired via the single-strand annealing (SSA) and non-homologous end-joining (NHEJ) pathways in a manner dependent on the availability of direct repeat sequences in the transgene. These results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, and confirm the utility of HEs in the manipulation and modification of transgenes in this important vector.

  19. Competência de peixes como predadores de larvas de Aedes aegypti, em condições de laboratório Efficacy of fish as predators of Aedes aegypti larvae, under laboratory conditions

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    Luciano Pamplona de Góes Cavalcanti

    2007-08-01

    Full Text Available OBJETIVO: Avaliar a competência de peixes na predação de larvas de Aedes aegypti, em condições de laboratório. MÉTODOS: Foram testados machos e fêmeas de cinco espécies de peixe. Os testes de predação duravam cinco semanas para cada espécie. Cada ensaio compreendia quatro caixas testes e quatro caixas controles. Das caixas controle, duas tinham somente um peixe e as outras duas, apenas larvas. Cada caixa teste continha um peixe e larvas. Na primeira semana foram expostas 100 larvas em cada caixa, e a cada semana acrescentavam-se 100 larvas por caixa/dia, até se obter um máximo de 500 larvas/dia. Comprimento e peso dos peixes foram medidos semanalmente. RESULTADOS: Foram utilizadas 369.000 larvas no total. O Trichogaster trichopteros foi a única espécie em que ambos os sexos predaram 100% das larvas oferecidas. O Betta splendens deixou de predar apenas 15 larvas. Machos do Poecilia reticulata apresentaram baixa capacidade larvófaga quando comparados às fêmeas da mesma espécie. Em relação ao peso e tamanho o Betta splendens mostrou-se capaz de predar 523 larvas/grama/dia. CONCLUSÕES: Fêmeas e machos de Trichogaster trichopteros e de Astyanax fasciatus, e fêmeas de Betta splendens e de Poecillia sphenops foram os peixes que apresentaram maior competência para predar as larvas. Embora com competência menor, machos de Poecillia sphenops e fêmeas de Poecilia reticulata foram capazes de eliminar o número de larvas de Aedes aegypti que possam emergir durante 24 horas num criadouro, em condições naturais. Machos de Poecilia reticulata não foram predadores eficazes.OBJECTIVE: To evaluate the efficacy of fish as predators of the Aedes aegypti larvae in laboratory conditions. METHODS: The male and female of five different fish were included in the experiment. The tests to measure their consumption ability lasted five weeks for each species. Each trial involved four test tanks and four control tanks. Two control tanks

  20. Imidacloprid impairs the post-embryonic development of the midgut in the yellow fever mosquito Stegomyia aegypti (=Aedes aegypti).

    Science.gov (United States)

    Fernandes, K M; Gonzaga, W G; Pascini, T V; Miranda, F R; Tomé, H V V; Serrão, J E; Martins, G F

    2015-09-01

    The mosquito Stegomyia aegypti (=Aedes aegypti) (Diptera: Culicidae) is a vector for the dengue and yellow fever viruses. As blood digestion occurs in the midgut, this organ constitutes the route of entry of many pathogens. The effects of the insecticide imidacloprid on the survival of St. aegypti were investigated and the sub-lethal effects of the insecticide on midgut development were determined. Third instar larvae were exposed to different concentrations of imidacloprid (0.15, 1.5, 3.0, 6.0 and 15.0 p.p.m.) and survival was monitored every 24 h for 10 days. Midguts from imidacloprid-treated insects at different stages of development were dissected and processed for analyses by transmission electron microscopy, immunofluorescence microscopy and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assays. Imidacloprid concentrations of 3.0 and 15.0 p.p.m. were found to affect midgut development similarly. Digestive cells of the fourth instar larvae (L4) midgut exposed to imidacloprid had more multilamellar bodies, abundantly found in the cell apex, and more electron-lucent vacuoles in the basal region compared with those from untreated insects. Moreover, imidacloprid interfered with the differentiation of regenerative cells, dramatically reducing the number of digestive and endocrine cells and leading to malformation of the midgut epithelium in adults. The data demonstrate that imidacloprid can reduce the survival of mosquitoes and thus indicate its potentially high efficacy in the control of St. aegypti populations. © 2015 The Royal Entomological Society.

  1. PEMETAAN, KARAKTERISTIK HABITAT DAN STATUS RESISTENSI Aedes aegypti DI KOTA BANJARMASIN KALIMANTAN SELATAN

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    Safitri -

    2013-12-01

    Full Text Available ABSTRACT. Control program of Aedes aegypti in Banjarmasin by using Malation has been done since almost 15 years ago. Related to this, a study about distribution and resistence of Ae.aegypti inBanjarmasin has been done. Ae.aegypti shown to be in almost all area in Banjarmasin, with water container in the bathroom and in the house are more liked. Susceptibility test showed thatthis mosquito was resistence to Malation 0,8%. Therefor, a policy to change this type of insecticide is needed.Key words : Ae.aegypti, resistence, dengue fever, Malation ABSTRAKProgram pengendalian nyamuk Aedes aegypti di Banjarmasin dengan menggunakan Malation telah dilakukan sejak hampir 15 tahun lalu. Terkait hal ini, sebuah studi tentang distribusi dan resistensi Ae. aegypti di Banjarmasin telah dilakukan. Ae. aegypti ditemukan di hampir semua wilayah di Banjarmasin dan lebih menyukai bak mandi dan penampungan air lainnya di dalam rumah. Uji Kerentanan menunjukkan bahwa nyamukini resisten terhadap Malation 0,8%. Maka, kebijakan untuk mengubah jenis insektisida yang digunakan sangat dibutuhkan Kata kunci: Ae. aegypti, resistensi, demam berdarah, Malation

  2. Proof of concept for a novel insecticide bioassay based on sugar feeding by adult Aedes aegypti (Stegomyia aegypti).

    Science.gov (United States)

    Stell, F M; Roe, R M; Arellano, C; Kennedy, L; Thornton, H; Saavedra-Rodriguez, K; Wesson, D M; Black, W C; Apperson, C S

    2013-09-01

    Aedes aegypti L. (Stegomyia aegypti) (Diptera: Culicidae) is the principal vector of dengue and yellow fever viruses in tropical and subtropical regions of the world. Disease management is largely based on mosquito control achieved by insecticides applied to interior resting surfaces and through space sprays. Population monitoring to detect insecticide resistance is a significant component of integrated disease management programmes. We developed a bioassay method for assessing insecticide susceptibility based on the feeding activity of mosquitoes on plant sugars. Our prototype sugar-insecticide feeding bioassay system was composed of inexpensive, disposable components, contained minimal volumes of insecticide, and was compact and highly transportable. Individual mosquitoes were assayed in a plastic cup that contained a sucrose-permethrin solution. Trypan blue dye was added to create a visual marker in the mosquito's abdomen for ingested sucrose-permethrin solution. Blue faecal spots provided further evidence of solution ingestion. With the sugar-insecticide feeding bioassay, the permethrin susceptibility of Ae. aegypti females from two field-collected strains was characterized by probit analysis of dosage-response data. The field strains were also tested by forced contact of females with permethrin residues on filter paper. Dosage-response patterns were similar, indicating that the sugar-insecticide feeding bioassay had appropriately characterized the permethrin susceptibility of the two strains. © 2012 The Royal Entomological Society.

  3. Hydroids (Cnidaria, Hydrozoa of the intertidal zone of Governador and Paquetá islands, Guanabara Bay, Rio de Janeiro, Brazil Hidróides (Cnidaria, Hydrozoa da zona entremarés das ilhas do Governador e Paquetá, Baía de Guanabara, Rio de Janeiro, Brasil

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    Priscila A. Grohmann

    2009-09-01

    Full Text Available During six consecutive months, sampling were made at three points located on Governador Island and three on Paquetá Island in Guanabara Bay, Rio de Janeiro, Brazil. Material was collected from dock pilings and rocks in the intertidal zone. In these samples, five species belonging to three families, Corynidae, Kirchenpaueriidae and Campanulariidae, were identified. The campanulariid species Obelia dichotoma Linnaeus, 1758, dominated at nearly all points sampled. The small number of species obtained in this survey is attributed to the intense pollution in the bay, which borders the second-largest industrial complex and the second-largest demographic center of Brazil.Durante seis meses consecutivos foram feitas coletas em três pontos localizados na ilha do Governador e três em Paquetá, sendo colhido material em pilares de cais e rochas na zona entremarés. Foram identificadas cinco espécies pertencentes a três famílias: Corynidae, Kirchenpaueriidae e Campanulariidae. A espécie Obelia dichotoma Linnaeus, 1758 dominou em praticamente todos os pontos. O pequeno número de espécies encontradas neste levantamento deve-se, provavelmente, ao alto grau de poluição atualmente existente na baía, uma vez que ela abriga o segundo maior polo industrial e o segundo maior centro demográfico do Brasil.

  4. Dimensioning of a photovoltaic-wind hybrid system in UFRJ campus of the Ilha do Fundao; Dimensionamento de um sistema hibrido eolico-fotovoltaico na UFRJ Campus Ilha do Fundao

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Silvio Carlos Anibal de [Universidade Federal do Rio de Janeiro (DEM/UFRJ), RJ (Brazil). Dept. de Engenharia Mecanica], e-mail: silvioa@gmail.com; Santos, Rodrigo Zimmer de Abreu; Argolo, Felipe Henrique Bohm; Danziger, Fernando Artur Ragoni; Sollero, Luiz Paulo Vervloet; Castro, Marcial Pereira Saboya de [Universidade Federal do Rio de Janeiro (DEM/UFRJ), RJ (Brazil). Dept. de Engenharia Industrial], e-mail: rodzimmer@gmail.com, e-mail: felipeargolo@yahoo.com.br, e-mail: ragonziger@gmail.com, e-mail: lpsollero@gmail.com, e-mail: marcial.saboya@gmail.com

    2008-07-01

    A techno-economic analysis of a grid connected photovoltaic-wind hybrid energy system is done in the present paper. The set-up consists of photovoltaic solar-cell arrays, wind generators, a lead acid storage batteries and an inverter unit to convert DC power to AC power. The study has been performed with the simulation tool HOMER (Hybrid Optimization Model for Electric Renewable) developed by the National Renewable Energy Laboratory (NREL) for techno-economical feasibility studies of hybrid systems. The system is connected to the electrical grid in order to supply all the electrical energy demand of the Technological Centre of the Federal University of Rio de Janeiro. Many systems configurations have been considered and it was possible to simulate the hourly operation of each system and to calculate technical, economic and environmental performance parameters. The best configuration is a Fuhrlander 30 wind generator, associated to a photovoltaic system to reach the generation of 96% of the energy demand by means of renewable sources. (author)

  5. Occurrence of Toxorhynchites guadeloupensis (Dyar and Knab) in oviposition trap of Aedes aegypti (L.) (Diptera: Culicidae)

    International Nuclear Information System (INIS)

    Honorio, Nildimar A.

    2007-01-01

    Toxorhynchites guadeloupensis (Dyar and Knab), a poorly known mosquito species, was observed preying upon Aedes aegypti (L.) larvae, in an oviposition trap placed for routine dengue entomological surveillance, during 2003-2004 in the urban area of Boa Vista, Roraima, Brazil. This is the first report for Tx. guadeloupensis using Ae. aegypti oviposition traps as breeding places. This finding may have important consequences in the epidemiology and local dengue control since Ae. aegypti density is a basic variable in dengue prediction. Whether predation of Ae aegypti by Tx. guadeloupensis in the Amazon is of significance, is a question to be examined. Also, larval predation may be a cause for underestimation of the actual Ae aegypti numbers. Together these hypotheses need to be better investigated as they are directly related to dengue epidemiology, to the success of any outbreak prediction and surveillance program. (author)

  6. The wMel strain of Wolbachia Reduces Transmission of Zika virus by Aedes aegypti.

    Science.gov (United States)

    Aliota, Matthew T; Peinado, Stephen A; Velez, Ivan Dario; Osorio, Jorge E

    2016-07-01

    Zika virus (ZIKV) is causing an explosive outbreak of febrile disease in the Americas. There are no effective antiviral therapies or licensed vaccines for this virus, and mosquito control strategies have not been adequate to contain the virus. A promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium Wolbachia into Aedes aegypti mosquitoes. This primarily has been proposed as a tool to control dengue virus (DENV) transmission; however, evidence suggests Wolbachia infections confer protection for Ae. aegypti against other arboviruses. At present, it is unknown whether or not ZIKV can infect, disseminate, and be transmitted by Wolbachia-infected Ae. aegypti. Using Ae. aegypti infected with the wMel strain of Wolbachia that are being released in Medellin, Colombia, we report that these mosquitoes have reduced vector competence for ZIKV. These results support the use of Wolbachia biocontrol as a multivalent strategy against Ae. aegypti-transmitted viruses.

  7. Occurrence of Toxorhynchites guadeloupensis (Dyar and Knab) in oviposition trap of Aedes aegypti (L.) (Diptera: Culicidae)

    Energy Technology Data Exchange (ETDEWEB)

    Honorio, Nildimar A. [Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ (Brazil). Dept. de Entomologia. Lab. de Transmissores de Hematozoarios; Barros, Fabio S.M. de [Universidade Federal de Roraima (UFRR), Boa Vista, RR (Brazil). Centro de Ciencias Biologicas e da Saude. Nucleo Avancado de Vetores; Tsouris, Pantelis; Rosa-Freitas, Maria G. [Freitas and Tsouris Consultants, Spata-Attikis (Greece)]. E-mail: maria@freitas-tsouris.com

    2007-09-15

    Toxorhynchites guadeloupensis (Dyar and Knab), a poorly known mosquito species, was observed preying upon Aedes aegypti (L.) larvae, in an oviposition trap placed for routine dengue entomological surveillance, during 2003-2004 in the urban area of Boa Vista, Roraima, Brazil. This is the first report for Tx. guadeloupensis using Ae. aegypti oviposition traps as breeding places. This finding may have important consequences in the epidemiology and local dengue control since Ae. aegypti density is a basic variable in dengue prediction. Whether predation of Ae aegypti by Tx. guadeloupensis in the Amazon is of significance, is a question to be examined. Also, larval predation may be a cause for underestimation of the actual Ae aegypti numbers. Together these hypotheses need to be better investigated as they are directly related to dengue epidemiology, to the success of any outbreak prediction and surveillance program. (author)

  8. Parental care behavior in the Guiana dolphin, Sotalia guianensis (Cetacea: Delphinidae, in Ilha Grande Bay, southeastern Brazil

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    Rodrigo H. O. Tardin

    2013-02-01

    Full Text Available Parental care is any form of parental behavior that increases offspring fitness. To the authors' knowledge, this study is the first to analyze the intensity of parental care in the Guiana dolphin, Sotalia guianensis (van Bénéden, 1864. The objectives of this study are as follows: 1 to quantify the degree of parental care in S. guianensis in Ilha Grande Bay, Rio de Janeiro; 2 to investigate the influence of behavioral state and group size on the degree of parental care; and 3 to evaluate the differences between the intensity of parental care provided to calves and juveniles. Our results indicate that the intensity of parental care is high in S. guianensis and that care is more intense in larger groups. It is possible that these differences serve to maximize hydrodynamic gains and to minimize risks. Our results suggest that parental care is more intense during travel. A possible reason for this greater intensity is that the feeding dynamics show a more random pattern than other behavioral states. Moreover, the results indicate that calves receive more intense care than juveniles. These results suggest that parent-offspring conflict is possible in the study population.

  9. Cuidado parental de Sula leucogaster (Boddaert (Aves, Pelecaniformes, Sulidae nas Ilhas dos Currais, Paraná, Brasil

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    Martins Flávia de Campos

    2003-01-01

    Full Text Available In Sula leucogaster (Boddaert, 1783 it happens a brood reduction from two to one egg. The eggs hatch asynchronously and it is rare for the second chick to survive for long. A quantify parental investment is proposed and the differences between males and females of Sula leucogaster is verified for to help to understand the brood reduction. The studies were conducted on Ilhas dos Currais, Paraná, Brazil, where a total of 140 nests were studied. Males and females shared the breeding care period. However, females invested about 8% more on incubation than males (c² = 7,37; c² critic = 3,842. Time of absence in the nest for females was larger than for males (c² = 13,9; c² critic = 3,842. This is probably a function of females bringing food to chicks more frequently (61%, n = 120 than males (c² = 5,63; c² critic = 3,842. The main parental investment during the phases with eggs and/or hatchings was protection, and during the postfledging period was to provide feeding. The brood reduction in S. leucogaster is due to the high cost of rearing two chicks during the same breeding attempt. This could be a disadvantage to the future survival and/or the reproduction of both parents and chicks.

  10. Taiwan: um futuro formoso para a Ilha? Aspectos de segurança e política

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    Paulo A. Pereira Pinto

    2004-12-01

    Full Text Available A partir de declaração de Lee Teng-Hui, em 1999, de que existe "um Estado em cada margem do estreito", foi estabelecida nova cena de partida que dificulta a realização de um futuro formoso para a ilha de Taiwan. Em março de 2004, por ocasião de eleições para renovar sua liderança, os taiwaneses defrontaram-se com problemas de segurança e políticos, influenciados por poderosas forças externas, que contribuem para o clima de incerteza que se vive. Parece difícil crer que autoridades em Taipé sejam capazes do engenho e arte de exercitar a ambigüidade necessária para manter o "status quo".Lee Teng-Hui's 1999 statement that there is "one State on each side of the strait" established a new starting point that makes difficult the achievement of a rosy future for the island of Taiwan. In March 2004, as elections for renewing their leadership were taking place, the Taiwanese were confronted with security and political problems that were influenced by powerful external forces, which intensified the prevailing uncertainty. It seems difficult to believe that the authorities in Taipei possess the skill and the art for practicing the requisite ambiguity for maintaining the status quo on the island.

  11. Occurrence of Lutzomyia longipalpis (Phlebotominae) and canine visceral leishmaniasis in a rural area of Ilha Solteira, SP, Brazil.

    Science.gov (United States)

    Spada, Julio Cesar Pereira; Silva, Diogo Tiago da; Martins, Kennya Rozy Real; Rodas, Lílian Aparecida Colebrusco; Alves, Maria Luana; Faria, Glaucia Amorim; Buzutti, Marcelo Costa; Silva, Hélio Ricardo; Starke-Buzetti, Wilma Aparecida

    2014-01-01

    This study aimed to investigate the occurrence of Lutzomyia longipalpis and also the canine visceral leishmaniasis (CVL) in a rural area of Ilha Solteira, state of São Paulo. Blood samples were collected from 32 dogs from different rural properties (small farms) and were analyzed by ELISA and the indirect immunofluorescence antibody test (IFAT) in order to diagnose CVL. From these serological tests, 31.25% of the dogs were positive for CVL and these were distributed in 66.7% (8/12) of the rural properties, which were positive for L. longipalpis. CDC (Center for Disease Control and Prevention) light traps were installed in 12 properties (one per property) and insects were caught on three consecutive days per month for one year. L. longipalpis was present on 100% of the rural properties visited, at least once during the twelve-month interval, totaling 64 males and 25 females. The insects were more numerous after the peak of the rain, but the association between prevalence of peridomestic vectors and the climatic data (precipitation, relative air humidity and temperature) and the occurrences of CVL among dogs on each rural property were not statistical significant (p longipalpis indicate that more attention is necessairy for the control of this disease in the rural area studied.

  12. Morphology of the shell of Happiella cf. insularis (Gastropoda: Heterobranchia: Systrophiidae from three forest areas on Ilha Grande, Southeast Brazil

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    Amilcar Brum Barbosa

    2014-06-01

    Full Text Available We conducted a study on shell morphology variation among three populations of Happiella cf. insularis (Boëttger, 1889 inhabiting different areas (Jararaca, Caxadaço, and Parnaioca trails at Vila Dois Rios, Ilha Grande, Angra dos Reis, state of Rio de Janeiro, Brazil. Linear and angular measurements, shell indices representing shell shape, and whorl counts were obtained from images drawn using a stereomicroscope coupled with a camera lucida. The statistical analysis based on ANOVA (followed by Bonferroni's test, Pearson's correlation matrix, and discriminant analysis enabled discrimination among the populations studied. The variable that most contributed to discriminate among groups was shell height. Mean shell height was greatest for specimens collected from Jararaca, probably reflecting the better conservation status of that area. Good conservation is associated with enhanced shell growth. Mean measurements were smallest for specimens from Parnaioca, the most disturbed area surveyed. Mean aperture height was smallest for specimens from Parnaioca, which may represent a strategy to prevent excessive water loss. Discriminant analysis revealed that the snails from Jararaca differ the most from snails collected in the two other areas, reflecting the different conservation status of these areas: shells reach larger sizes in the localities where the humidity is higher. The similarities in shell morphology were greater between areas that are more similar environmentally (Caxadaço and Parnaioca, suggesting that conchological differences may correspond to adaptations to the environment.

  13. Potential for biocontrol of melanized fungi by actinobacteria isolated from intertidal region of Ilha Do Mel, Paraná, Brazil.

    Science.gov (United States)

    Dalitz, Camila de Araújo; Porsani, Mariana Vieira; Figel, Izabel Cristina; Pimentel, Ida C; Dalzoto, Patrícia R

    Actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. Identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16S region of ribosomal DNA (rDNA), which is conserved and present in all bacteria, is an important method of identification. Melanized fungi are free-living organisms, which can also be pathogens of clinical importance. This work aimed to evaluate growth inhibition of melanized fungi by actinobacteria and to identify the latter to the species level. In this study, antimicrobial activity of 13 actinobacterial isolates from the genus Streptomyces was evaluated against seven melanized fungi of the genera Exophiala, Cladosporium, and Rhinocladiella. In all tests, all actinobacterial isolates showed inhibitory activity against all isolates of melanized fungi, and only one actinobacterial isolate had less efficient inhibitory activity. The 16S rDNA region of five previously unidentified actinobacterial isolates from Ilha do Mel, Paraná, Brazil, was sequenced; four of the isolates were identified as Streptomyces globisporus subsp. globisporus, and one isolate was identified as Streptomyces aureus. This work highlights the potential of actinobacteria with antifungal activity and their role in the pursuit of novel antimicrobial substances. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  14. Shifting patterns of Aedes aegypti fine scale spatial clustering in Iquitos, Peru.

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    Genevieve LaCon

    2014-08-01

    Full Text Available Empiric evidence shows that Aedes aegypti abundance is spatially heterogeneous and that some areas and larval habitats produce more mosquitoes than others. There is a knowledge gap, however, with regards to the temporal persistence of such Ae. aegypti abundance hotspots. In this study, we used a longitudinal entomologic dataset from the city of Iquitos, Peru, to (1 quantify the spatial clustering patterns of adult Ae. aegypti and pupae counts per house, (2 determine overlap between clusters, (3 quantify the temporal stability of clusters over nine entomologic surveys spaced four months apart, and (4 quantify the extent of clustering at the household and neighborhood levels.Data from 13,662 household entomological visits performed in two Iquitos neighborhoods differing in Ae. aegypti abundance and dengue virus transmission was analyzed using global and local spatial statistics. The location and extent of Ae. aegypti pupae and adult hotspots (i.e., small groups of houses with significantly [p<0.05] high mosquito abundance were calculated for each of the 9 entomologic surveys. The extent of clustering was used to quantify the probability of finding spatially correlated populations. Our analyses indicate that Ae. aegypti distribution was highly focal (most clusters do not extend beyond 30 meters and that hotspots of high vector abundance were common on every survey date, but they were temporally unstable over the period of study.Our findings have implications for understanding Ae. aegypti distribution and for the design of surveillance and control activities relying on household-level data. In settings like Iquitos, where there is a relatively low percentage of Ae. aegypti in permanent water-holding containers, identifying and targeting key premises will be significantly challenged by shifting hotspots of Ae. aegypti infestation. Focusing efforts in large geographic areas with historically high levels of transmission may be more effective than

  15. Shifting patterns of Aedes aegypti fine scale spatial clustering in Iquitos, Peru.

    Science.gov (United States)

    LaCon, Genevieve; Morrison, Amy C; Astete, Helvio; Stoddard, Steven T; Paz-Soldan, Valerie A; Elder, John P; Halsey, Eric S; Scott, Thomas W; Kitron, Uriel; Vazquez-Prokopec, Gonzalo M

    2014-08-01

    Empiric evidence shows that Aedes aegypti abundance is spatially heterogeneous and that some areas and larval habitats produce more mosquitoes than others. There is a knowledge gap, however, with regards to the temporal persistence of such Ae. aegypti abundance hotspots. In this study, we used a longitudinal entomologic dataset from the city of Iquitos, Peru, to (1) quantify the spatial clustering patterns of adult Ae. aegypti and pupae counts per house, (2) determine overlap between clusters, (3) quantify the temporal stability of clusters over nine entomologic surveys spaced four months apart, and (4) quantify the extent of clustering at the household and neighborhood levels. Data from 13,662 household entomological visits performed in two Iquitos neighborhoods differing in Ae. aegypti abundance and dengue virus transmission was analyzed using global and local spatial statistics. The location and extent of Ae. aegypti pupae and adult hotspots (i.e., small groups of houses with significantly [pentomologic surveys. The extent of clustering was used to quantify the probability of finding spatially correlated populations. Our analyses indicate that Ae. aegypti distribution was highly focal (most clusters do not extend beyond 30 meters) and that hotspots of high vector abundance were common on every survey date, but they were temporally unstable over the period of study. Our findings have implications for understanding Ae. aegypti distribution and for the design of surveillance and control activities relying on household-level data. In settings like Iquitos, where there is a relatively low percentage of Ae. aegypti in permanent water-holding containers, identifying and targeting key premises will be significantly challenged by shifting hotspots of Ae. aegypti infestation. Focusing efforts in large geographic areas with historically high levels of transmission may be more effective than targeting Ae. aegypti hotspots.

  16. TRANSMISI TRANSOVARIAL VIRUS DENGUE PADA TELUR NYAMUK AEDES AEGYPTI(L.

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    Magdalena Desiree Seran

    2013-03-01

    Full Text Available Abstract. The ability of dengue virus to maintain its existence in nature through two mechanisms, both horizontal and vertical transmission (transovarial of the infective female mosquitoes to the next generation. This study aims to investigate the transovarial transmission and transovarial infection rate (TIR of dengue virus in eggs Aedes aegypti infected mother has a peroral virus DEN-2. This study is an experimental study in the laboratory. The population of the study was Ae. aegypti adults who have previously been infected with DEN-2 virus orally and proved to be infected with DEN-2 transovarially (Fl. The research sample was egg of Ae. aegypti from F2 generation which colonized from DEN-2 transovarially infected Ae. aegypti (Fl. Egg squash preparations made as many as 50 samples from jive difJerent mosquito parents. The presence of dengue virus antigen in mosquitoes FO and Fl were checked by SPBC immunocytochemistry method and using monoclonal antibodies DSSC7 (l: 50 as standardized primary antibodies. The results shows the existence of transovarial transmission of dengue virus in eggs Ae. aegypti (F2 were seen in squash preparations in the form of a brownish color egg spread on embryonic tissues (TIR= 52%. It concludes that dengue virus is able to be transmitted vertically through the egg. Keywords: transovarial transmission, eggsquash, Aedes aegypti, transovarial infection rate (TIR Abstrak. Kemampuan virus dengue untuk mempertahankan keberadaanya di alam dilakukan melalui dua mekanisme yaitu transmisi horizontal dan dengan transmisi vertikal (transovarial yaitu dari nyamuk betina infektif ke generasi berikutnya. Penelitian ini bertujuan untuk mengetahui adanya transmisi transovarial dan transovarial infection rate (TIR virus dengue pada telur Ae. aegypti yang induknya telah diinfeksi virus DEN-2 secara peroraI. Penelitian merupakan jenis penelitian eksperimental di laboratorium. Populasi penelitian adalah Ae. aegypti betina dewasa yang

  17. Role of Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) in local dengue epidemics in Taiwan.

    Science.gov (United States)

    Tsai, Pui-Jen; Teng, Hwa-Jen

    2016-11-09

    Aedes mosquitoes in Taiwan mainly comprise Aedes albopictus and Ae. aegypti. However, the species contributing to autochthonous dengue spread and the extent at which it occurs remain unclear. Thus, in this study, we spatially analyzed real data to determine spatial features related to local dengue incidence and mosquito density, particularly that of Ae. albopictus and Ae. aegypti. We used bivariate Moran's I statistic and geographically weighted regression (GWR) spatial methods to analyze the globally spatial dependence and locally regressed relationship between (1) imported dengue incidences and Breteau indices (BIs) of Ae. albopictus, (2) imported dengue incidences and BI of Ae. aegypti, (3) autochthonous dengue incidences and BI of Ae. albopictus, (4) autochthonous dengue incidences and BI of Ae. aegypti, (5) all dengue incidences and BI of Ae. albopictus, (6) all dengue incidences and BI of Ae. aegypti, (7) BI of Ae. albopictus and human population density, and (8) BI of Ae. aegypti and human population density in 348 townships in Taiwan. In the GWR models, regression coefficients of spatially regressed relationships between the incidence of autochthonous dengue and vector density of Ae. aegypti were significant and positive in most townships in Taiwan. However, Ae. albopictus had significant but negative regression coefficients in clusters of dengue epidemics. In the global bivariate Moran's index, spatial dependence between the incidence of autochthonous dengue and vector density of Ae. aegypti was significant and exhibited positive correlation in Taiwan (bivariate Moran's index = 0.51). However, Ae. albopictus exhibited positively significant but low correlation (bivariate Moran's index = 0.06). Similar results were observed in the two spatial methods between all dengue incidences and Aedes mosquitoes (Ae. aegypti and Ae. albopictus). The regression coefficients of spatially regressed relationships between imported dengue cases and Aedes mosquitoes

  18. História da colonização do ratinho-caseiro, Mus musculus domesticus, em ilhas AtLânticas (Madeira, Açores e Cabo Verde): uma abordagem multilocus

    OpenAIRE

    Santos, Ana Sofia Carromeu dos

    2017-01-01

    Tese de mestrado, Biologia Evolutiva e do Desenvolvimento, Universidade de Lisboa, Faculdade de Ciências, 2017 As ilhas oceânicas são consideradas laboratórios naturais para o estudo da evolução. Os humanos são também agentes de colonização de várias espécies, nomeadamente, mamíferos terrestres, ao transportarem, ativa ou passivamente, estes animais para as ilhas. Uma das introduções mediadas pelo Homem mais icónicas envolve a disseminação mundial do ratinho-caseiro, principalmente da sub-...

  19. Infecção viral da vinha nos Biscoitos (ilha Terceira - Açores Grapevine virus infection at Biscoitos (Terceira island - Azores

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    Arícia Figueiredo

    2009-12-01

    Full Text Available A região dos Biscoitos na ilha Terceira, nos Açores, é mundialmente conhecida pela vinha em curraletas destinada à produção de Vinhos Licorosos de Qualidade Produzidos em Região Demarcada (VLQPRD. As castas predominantes são Verdelho branco e Verdelho roxo mas também estão presentes Terrantez, Arinto e Boal branco. Um dos objectivos do projecto Interfruta-II é avaliar o actual estado sanitário destas videiras em relação aos principais vírus. Assim, em Fevereiro de 2006, foi realizada uma prospecção em sete vinhas tendo sido analisados por ELISA os vírus GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-7, GFLV, ArMV, GVA, GVB e GFkV. Os resultados são analisados por casta e vinha. No global mostram que a infecção por GLRaV-3 atinge os 100% e que a infecção por um ou dois Nepovirus é superior a 50%. Os restantes vírus foram detectados em menor percentagem. Existe elevada percentagem de infecções mistas. Atendendo ao prejudicial efeito qualitativo e/ou quantitativo das infecções detectadas, é apresentada uma estratégia de intervenção fitossanitária pretendendo contribuir para a recuperação da vinha nos Biscoitos.At Terceira island in Azores, the region Biscoitos is well known by its vineyards in typical “curraletas”. The grapevines are for the production of VLQPRD (i.e. Quality Liquorus Wines Produced in Demarcated Region. The predominant varieties are Verdelho branco and Verdelho roxo but also Terrantez, Arinto and Boal branco are present. One of the objectives of the project Interfruta-II is the evaluation of the actual sanitary status of those grapevines in relation to the main viruses. Therefore, in February 2006, a survey was done in seven vineyards evaluating by ELISA the virus GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-7, GFLV, ArMV, GVA, GVB and GFkV. The results are analyzed by vineyard and by variety. In general they show that infection by GLRaV-3 is 100% and that infection by one or two Nepovirus is above 50%. The other

  20. Microbiological quality ambience, foods and water, in restaurants of Ilha do Mel, Paraná StateQualidade microbiológica do ambiente, alimentos e água, em restaurantes da Ilha do Mel/PR

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    Ronaldo Tamanini

    2012-05-01

    Full Text Available Each year, several outbreaks of foodborne diseases are reported in Parana state, Brazil. Bacteria are responsible for 70% of these outbreaks and 95% of cases of food poisoning. There are reports that kitchen equipment and utensils contaminated have participated of approximately 16% of outbreaks. The aim of this study was to evaluate the microbiological quality of water, fresh foods, frozen foods, ready to eat food and sanitary conditions of three restaurants in Ilha do Mel – Paraná, Brazil, using PetrifilmTM. The results showed that 72.2% surfaces of had unsatisfactory sanitary conditions. Cutting boards, scrub sponge, kitchen sinks and dish towels had the highest average contamination. According to Brazilian law, samples of food outside of the standard were: seafood sauce, with 7,0 x 102 CFU/g of E. Coli, frozen mussels, with 7,0 x 102 CFU/g of E. coli; sample lettuce and grated carrot, considered clean, with 1,0 x 103 e 2,0 x 105 CFU/g of EC, respectively. All samples of food, in general, had high bacterial counts. Vegetables were the main responsible for the contamination of kitchen boards and sinks. The water used in the three restaurants had satisfactory microbiological quality. Better hygienic sanitary conditions were found in the restaurant where the employees had training in good manufacturing practices. Vários surtos de Doenças Transmitidas por Alimentos são notificadas por ano no estado do Paraná, sendo as bactérias responsáveis por 70% destes surtos e 95% dos casos de toxinfecções alimentares. Existem relatos de que utensílios e equipamentos contaminados participam de, aproximadamente, 16% dos surtos. O objetivo deste trabalho foi avaliar a qualidade microbiológica da água, dos alimentos in natura, congelados e expostos ao consumo e as condições higiênico-sanitárias de três restaurantes da Ilha do Mel/PR. Para produtos de origem animal, foi determinada a contagem de aeróbios mesófilos, coliformes totais, E. coli e

  1. Diversidade microbiana e biodegradação de hidrocarbonetos de petróleo em solos da ilha da trindade submetidos à bioestimulação

    OpenAIRE

    Camacho Montealegre, Celia Marcela

    2014-01-01

    Esse estudo analisou a diversidade microbiana de solos rizosféricos de duas espécies vegetais, Bulbostylis nesiotis (Hemsl.) C.B.Clarke e Cyperus atlanticus (Hemsl.), em dois tipos de solo (um franco-arenoso e um argiloso), provenientes de dois pontos de coleta da Ilha da Trindade - Brasil, durante um experimento de biorremediação em microcosmos. O experimento consistiu num fatorial com dois níveis (Presença/Ausência), dois fatores (Petróleo e Fertilização com NPK) e três repetições, num deli...

  2. Plantas daninhas em ilhas de vegetação em processo de regeneração natural Weeds on vegetation islands under natural regeneration

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    P.F Souza

    2011-12-01

    Full Text Available Neste trabalho é apresentada a composição florística e estrutural de ilhas de vegetação de um inselberg do Vale do Moxuara, localizado no município de Cariacica-Espírito Santo, onde avaliou-se a riqueza das ilhas de vegetação está relacionada com seu tamanho e com a profundidade do solo. Foram avaliadas 45 ilhas de vegetação para estimativa dos parâmetros fitossociológicos necessários às análises. Foram encontradas 53 espécies de plantas vasculares, pertencentes a 24 famílias e 48 gêneros. As famílias com maior representatividade foram Poaceae, com sete espécies, seguida de Asteraceae, Malvaceae e Fabaceae, com seis espécies cada. Plantas daninhas representaram 39,6% da flora inventariada e 25,5% da cobertura vegetal das ilhas. As espécies com maior frequência e cobertura relativa foram Selaginella convoluta e Anemia tomentosa. A regressão múltipla não se mostrou significativa, o que foi discutido considerando o papel inibidor de espécies daninhas.This study presents the floristic and structural composition of vegetation islands of an inselberg in Vale do Moxuara, in Cariacica-ES, to evaluate whether the islands' richness is related to their size and soil depth. A total of 45 vegetation islands were evaluated by estimating the phytosociological parameters needed for the analysis. Fifty-three vascular plant species of twenty-four families and forty-eight genders were found. The most outstanding families were Poaceae, with seven species, followed by Malvaceae and Fabaceae, with six species each. Weeds represented 39.6% of all flora listed and 25.5% of the islands' vegetation cover. Selaginella convoluta and Anemia tomentosa had the most frequency and relative cover. Multiple regression was not significant, considering the inhibiting role played by the weed species.

  3. Evaluación del desempeño de la planta de tratamiento de aguas residuales urbanas de ILHA Solteira (SP) por lagunas facultativas primarias

    OpenAIRE

    Iván Andrés Sánchez Ortiz; Tsunao Matsumoto

    2012-01-01

    El objetivo principal de esta investigación fue evaluar el desempeño de la Planta de Tratamiento de Aguas Residuales de Ilha Solteira-SP durante las diferentes épocas climáticas del año. Se realizó un levantamiento batimétrico de las lagunas facultativas primarias, un monitoreo de 24 horas y el seguimiento de su desempeño en 3 etapas de colecta de 3 meses de duración cada una. La planta tuvo cerca del 40% de su volumen efectivo ocupado por lodos; el efluente final registró una eficiencia medi...

  4. Mapping the spatial distribution of Aedes aegypti and Aedes albopictus.

    Science.gov (United States)

    Ding, Fangyu; Fu, Jingying; Jiang, Dong; Hao, Mengmeng; Lin, Gang

    2018-02-01

    Mosquito-borne infectious diseases, such as Rift Valley fever, Dengue, Chikungunya and Zika, have caused mass human death with the transnational expansion fueled by economic globalization. Simulating the distribution of the disease vectors is of great importance in formulating public health planning and disease control strategies. In the present study, we simulated the global distribution of Aedes aegypti and Aedes albopictus at a 5×5km spatial resolution with high-dimensional multidisciplinary datasets and machine learning methods Three relatively popular and robust machine learning models, including support vector machine (SVM), gradient boosting machine (GBM) and random forest (RF), were used. During the fine-tuning process based on training datasets of A. aegypti and A. albopictus, RF models achieved the highest performance with an area under the curve (AUC) of 0.973 and 0.974, respectively, followed by GBM (AUC of 0.971 and 0.972, respectively) and SVM (AUC of 0.963 and 0.964, respectively) models. The simulation difference between RF and GBM models was not statistically significant (p>0.05) based on the validation datasets, whereas statistically significant differences (p<0.05) were observed for RF and GBM simulations compared with SVM simulations. From the simulated maps derived from RF models, we observed that the distribution of A. albopictus was wider than that of A. aegypti along a latitudinal gradient. The discriminatory power of each factor in simulating the global distribution of the two species was also analyzed. Our results provided fundamental information for further study on disease transmission simulation and risk assessment. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Especificidade da armadilha Adultrap para capturar fêmeas de Aedes aegypti (Diptera: Culicidae Specificity of the Adultrap for capturing females of Aedes aegypti (Diptera: Culicidae

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    Almério de Castro Gomes

    2007-04-01

    Full Text Available A Adultrap é uma nova armadilha feita para capturar fêmeas de Aedes aegypti. Foram realizados testes para avaliar sua especificidade tendo como referência a técnica da aspiração da espécie em abrigos artificiais. A Adultrap ficou exposta por 24 horas no intradomicílio e peridomicílio de 120 casas sorteadas em dois bairros da Cidade de Foz do Iguaçu, Estado do Paraná. O teste estatístico foi o modelo log-linear de Poisson. O resultado foi a captura de 726 mosquitos Culicidae, dos quais 80 eram Aedes aegypti. A Adultrap capturou apenas fêmeas desta espécie, enquanto o aspirador os dois sexos de Aedes aegypti e mais cinco outras espécies. A Adultrap capturou Aedes aegypti dentro e fora das casas, mas a análise indicou que no peridomicílio a armadilha capturou significantemente mais fêmeas do que a aspiração. Também, ficou evidenciada a sensibilidade da Adultrap para detectar Aedes aegypti em situação de baixa freqüência.The Adultrap is a new trap built for capturing females of Aedes aegypti. Tests were carried out to evaluate the specificity of this trap in comparison with the technique of aspiration of specimens in artificial shelters. Adultraps were kept for 24 hours inside and outside 120 randomly selected homes in two districts of the city of Foz do Iguaçú, State of Paraná. The statistical test was Poisson’s log-linear model. The result was 726 mosquitoes captured, of which 80 were Aedes aegypti. The Adultrap captured only females of this species, while the aspiration method captured both sexes of Aedes aegypti and another five species. The Adultrap captured Aedes aegypti inside and outside the homes, but the analysis indicated that, outside the homes, this trap captured significantly more females than aspiration did. The sensitivity of the Adultrap for detecting females of Aedes aegypti in low-frequency situations was also demonstrated.

  6. Larvicidal activity of some Cerrado plant extracts against Aedes aegypti

    OpenAIRE

    Rodrigues, A.M.S.; De Paula, J.E.; Dégallier, Nicolas; Molez, Jean-François; Espindola, L.S.

    2006-01-01

    One hundred ninety hexanic and ethanolic extracts from 27 plant species from the Cerrado biome of Brazil were tested for larvicidal activity against 3rd-stage Aedes aegypti larvae at 500 mu g/ml. Fourteen extracts from 7 species showed activity (> 65% mortality) against the larvae. Of these, Duguetia furfuracea, Piptocarpha rotundifolia, Casearia sylvestris var. lingua, Serjania lethalis, and Xylopia aromatica were active at 56.6, 162.31, 232.4, 285.76, and 384.37 mu g/ml, respectively. Annon...

  7. Aedes aegypti Larvicidal Sesquiterpene Alkaloids from Maytenus oblongata.

    Science.gov (United States)

    Touré, Seindé; Nirma, Charlotte; Falkowski, Michael; Dusfour, Isabelle; Boulogne, Isabelle; Jahn-Oyac, Arnaud; Coke, Maïra; Azam, Didier; Girod, Romain; Moriou, Céline; Odonne, Guillaume; Stien, Didier; Houël, Emeline; Eparvier, Véronique

    2017-02-24

    Four new sesquiterpene alkaloids (1-4) with a β-dihydroagrofuran skeleton and a new triterpenoid (5) were isolated from an ethyl acetate extract of Maytenus oblongata stems. Their structures were elucidated using 1D and 2D NMR spectroscopy as well as MS and ECD experiments. The M. oblongata stem EtOAc extract and the pure compounds isolated were tested for larvicidal activity against Aedes aegypti under laboratory conditions, and compounds 2 and 3 were found to be active.

  8. [Potential of Bacillus thuringiensis israelensis Berliner for controlling Aedes aegypti].

    Science.gov (United States)

    Polanczyk, Ricardo Antonio; Garcia, Marcelo de Oliveira; Alves, Sérgio Batista

    2003-12-01

    The importance of the entomopathogenic bacterium Bacillus thuringiensis israelensis in the control of Aedes aegypti is presented. The use and potential of B. thuringiensis israelensis against the mosquito vector of dengue fever is described. Other aspects such as insect's resistance development against chemicals and advantages and constraints of using microbial control are discussed. Emphasis is given to the importance of the use of this bacterium in Brazil, which could contribute significantly to solving the mosquito problem without affecting the environment, humans and others invertebrate organisms in critical regions.

  9. Dengue-1 virus and vector competence of Aedes aegypti (Diptera: Culicidae) populations from New Caledonia.

    Science.gov (United States)

    Calvez, Elodie; Guillaumot, Laurent; Girault, Dominique; Richard, Vaea; O'Connor, Olivia; Paoaafaite, Tuterarii; Teurlai, Magali; Pocquet, Nicolas; Cao-Lormeau, Van-Mai; Dupont-Rouzeyrol, Myrielle

    2017-08-09

    Dengue virus (DENV) is the arbovirus with the highest incidence in New Caledonia and in the South Pacific region. In 2012-2014, a major DENV-1 outbreak occurred in New Caledonia. The only known vector of DENV in New Caledonia is Aedes aegypti but no study has yet evaluated the competence of New Caledonia Ae. aegypti populations to transmit DENV. This study compared the ability of field-collected Ae. aegypti from different locations in New Caledonia to transmit the DENV-1 responsible for the 2012-2014 outbreak. This study also aimed to compare the New Caledonia results with the vector competence of Ae. aegypti from French Polynesia as these two French countries have close links, including arbovirus circulation. Three wild Ae. aegypti populations were collected in New Caledonia and one in French Polynesia. Female mosquitoes were orally exposed to DENV-1 (10 6 FFU/ml). Mosquito bodies (thorax and abdomen), heads and saliva were analyzed to measure infection, dissemination, transmission rates and transmission efficiency, at 7, 14 and 21 days post-infection (dpi), respectively. DENV-1 infection rates were heterogeneous, but dissemination rates were high and homogenous among the three Ae. aegypti populations from New Caledonia. Despite this high DENV-1 dissemination rate, the transmission rate, and therefore the transmission efficiency, observed were low. Aedes aegypti population from New Caledonia was less susceptible to infection and had lower ability to transmit DENV-1 than Ae. aegypti populations from French Polynesia. This study suggests that even if susceptible to infection, the New Caledonian Ae. aegypti populations were moderately competent vectors for DENV-1 strain from the 2012-2014 outbreak. These results strongly suggest that other factors might have contributed to the spread of this DENV-1 strain in New Caledonia and in the Pacific region.

  10. Determinasi Strain Aedes aegypti (Linn. yang Rentan Homozigot dengan Metode Seleksi Indukan Tunggal

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    Isfanda Isfanda

    2017-06-01

    Full Text Available Abstract. Aedes aegypti is a type of mosquito that can carry dengue virus, yellow fever and chikunguya. The spread of this mosquito is very broad, covering almost all tropical regions worldwide. This study aims to determine the vulnerability status of homozygous Ae. aegypti. Sample of Ae. aegypti is mosquito strain from Health Entomology Laboratory Bogor Institute of Agriculture and at random sampling. Ae. aegypti eggs which comes from the breeders hatched separately. Insecticide‐treated paper (impregnated paper malathion, bendiokarb and deltamethrin are use for insecticides testing using WHO test kit. The analysis showed that the mosquito Ae. aegypti tested with a single sib‐selection method and were exposed to the insecticide malathion, propoksur, and showed an increasing trend sipermetrin vulnerability homozygous at each generation. As for the fourth generation (F4 has not shown changes into a strain that is homozygous susceptible to three types of insecticides. The formation of homozygous susceptible strains take over five generations.Keywords: Ae. aegypti, malathion, bendiocarb, deltamethrin, single sib‐selection methodAbstrak. Aedes aegypti merupakan jenis nyamuk yang dapat membawa virus dengue, demam kuning (yellow fever dan chikunguya. Penyebaran nyamuk ini sangat luas, meliputi hampir semua daerah tropis di seluruh dunia. Penelitian ini bertujuan mengetahui status kerentanan nyamuk Ae. aegypti secara homozigot. Nyamuk Ae. aegypti yang dijadikan sampel yaitu nyamuk dewasa strain yang ada di Laboratorium Entomologi Kesehatan Institut Pertanian Bogor dan diambil secara acak. Telur Ae. aegypti yang berasal dari satu indukan ditetaskan secara terpisah. Insektisida yang digunakan untuk pengujian menggunakan kertas berinsektisida (impregnated paper malation, bendiokarb, dan deltametrin dengan menggunakan WHO test kit. Hasil analisis menunjukkan bahwa nyamuk Ae. aegypti yang diuji dengan metode seleksi indukan tunggal serta dipaparkan

  11. Larvicidal activity against Aedes aegypti of pacharin from Bauhinia acuruana.

    Science.gov (United States)

    da Silva Góis, Roberto Wagner; de Sousa, Leôncio Mesquita; Santiago, Gilvandete Maria Pinheiro; Romero, Nirla Rodrigues; Lemos, Telma Leda Gomes; Arriaga, Angela Martha Campos; Braz-Filho, Raimundo

    2013-07-01

    The aim of the present study was to evaluate the activity of pacharin isolated from the ethanol extract from roots of Bauhinia acuruana on third-instar larvae of Aedes aegypti Linn. (Diptera: Culicidae). The crude ethanol extract showed larvicidal activity at the concentration of 500 μg/mL. Given this larvicidal activity, this extract was submitted to chromatographic fractionation on a silica gel column eluted with n-hexane, dichloromethane, ethyl ether, ethyl acetate, and methanol in order to isolate the active compound(s). Pacharin, obtained in pure form from fraction eluted with ethyl ether, was evaluated for their larvicidal effects against A. aegypti. In these bioassays, the larvae were exposed at concentrations of 500, 250, 100, 50, and 25 μg/mL of the crude ethanol extract or pacharin. After 24 h, the number of dead larvae was counted and the LC₅₀ values for larval mortality were calculated. Pacharin showed LC50 value of 78.9 ± 1.8 μg/mL. The structure of isolated compound was identified on the basis of their spectral data (IR, 1D- and 2D-NMR) and by comparison with literature spectral data. The results indicate pacharin as a potential natural larvicide.

  12. The Aedes aegypti toll pathway controls dengue virus infection.

    Directory of Open Access Journals (Sweden)

    Zhiyong Xi

    2008-07-01

    Full Text Available Aedes aegypti, the mosquito vector of dengue viruses, utilizes its innate immune system to ward off a variety of pathogens, some of which can cause disease in humans. To date, the features of insects' innate immune defenses against viruses have mainly been studied in the fruit fly Drosophila melanogaster, which appears to utilize different immune pathways against different types of viruses, in addition to an RNA interference-based defense system. We have used the recently released whole-genome sequence of the Ae. aegypti mosquito, in combination with high-throughput gene expression and RNA interference (RNAi-based reverse genetic analyses, to characterize its response to dengue virus infection in different body compartments. We have further addressed the impact of the mosquito's endogenous microbial flora on virus infection. Our findings indicate a significant role for the Toll pathway in regulating resistance to dengue virus, as indicated by an infection-responsive regulation and functional assessment of several Toll pathway-associated genes. We have also shown that the mosquito's natural microbiota play a role in modulating the dengue virus infection, possibly through basal-level stimulation of the Toll immune pathway.

  13. Dengue virus replicates and accumulates in Aedes aegypti salivary glands

    Energy Technology Data Exchange (ETDEWEB)

    Raquin, Vincent, E-mail: vincent.raquin@univ-lyon1.fr [Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris (France); Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, 75015 Paris (France); Lambrechts, Louis, E-mail: louis.lambrechts@pasteur.fr [Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, 75015 Paris (France); Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, 75015 Paris (France)

    2017-07-15

    Dengue virus (DENV) is an RNA virus transmitted among humans by mosquito vectors, mainly Aedes aegypti. DENV transmission requires viral dissemination from the mosquito midgut to the salivary glands. During this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. Despite the implications for virus transmission and evolution, DENV replication in salivary glands has not been directly demonstrated. Here, we used a strand-specific quantitative RT-PCR assay to demonstrate that negative-strand DENV RNA is produced in Ae. aegypti salivary glands, providing conclusive evidence that viral replication occurs in this tissue. Furthermore, we showed that the concentration of DENV genomic RNA in salivary glands increases significantly over time, indicating that active replication likely replenishes DENV genetic diversity prior to transmission. These findings improve our understanding of the biological determinants of DENV fitness and evolution. - Highlights: •Strand-specific RT-qPCR allows accurate quantification of DENV (-) RNA in mosquito tissues. •Detection of DENV (-) RNA in salivary glands provides evidence of viral replication in this tissue. •Viral replication in salivary glands likely replenishes DENV genetic diversity prior to transmission.

  14. Dengue virus replicates and accumulates in Aedes aegypti salivary glands

    International Nuclear Information System (INIS)

    Raquin, Vincent; Lambrechts, Louis

    2017-01-01

    Dengue virus (DENV) is an RNA virus transmitted among humans by mosquito vectors, mainly Aedes aegypti. DENV transmission requires viral dissemination from the mosquito midgut to the salivary glands. During this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. Despite the implications for virus transmission and evolution, DENV replication in salivary glands has not been directly demonstrated. Here, we used a strand-specific quantitative RT-PCR assay to demonstrate that negative-strand DENV RNA is produced in Ae. aegypti salivary glands, providing conclusive evidence that viral replication occurs in this tissue. Furthermore, we showed that the concentration of DENV genomic RNA in salivary glands increases significantly over time, indicating that active replication likely replenishes DENV genetic diversity prior to transmission. These findings improve our understanding of the biological determinants of DENV fitness and evolution. - Highlights: •Strand-specific RT-qPCR allows accurate quantification of DENV (-) RNA in mosquito tissues. •Detection of DENV (-) RNA in salivary glands provides evidence of viral replication in this tissue. •Viral replication in salivary glands likely replenishes DENV genetic diversity prior to transmission.

  15. THE TRANSMISSION OF EQUINE ENCEPHALOMYELITIS VIRUS BY AEDES AEGYPTI.

    Science.gov (United States)

    Merrill, M H; Tenbroeck, C

    1935-10-31

    In confirming Kelser's work on the transmission of equine encephalomyelitis of the western type by Aëdes aegypti it has been learned that the mosquitoes must be fed virus of high titer if positive results are to be secured. A period of from 4 to 5 days after feeding either on infected guinea pigs or on brain containing virus must elapse before the disease is transmitted by biting, but after this time transmission regularly results for a period of about 2 months. By inoculation, virus can be demonstrated in the bodies of infected mosquitoes for the duration of life. Although virus multiplies in the mosquitoes and is generally distributed in their bodies, repeated attempts to demonstrate it in the eggs from females known to be infected as well as in larvae, pupae, and adults from such eggs have been uniformly negative. Larvae have not taken up virus added to the water in which they were living. Male mosquitoes have been infected with virus by feeding but they have not transmitted the virus to normal females, nor have males transmitted the virus from infected to normal females. When virus of the eastern instead of the western type is used transmission experiments with Aëdes aegypti are negative. Apparently this virus is incapable of penetrating the intestinal mucosa of the mosquito. If, however, it is inoculated into the body cavity by needle puncture it persists and transmission experiments are positive.

  16. Efficacy of topical permethrin as repellent against Aedes aegypti's bites.

    Science.gov (United States)

    Miot, Hélio Amante; Ferreira, Daniela Pinho; Mendes, Fabiana Guandalini; Carrenho, Flávia Roberta Hernandes; de Oliveira Amui, Isabela; Carneiro, Carlos Augusto Sá; Madeira, Newton Goulart

    2008-07-15

    Mosquitoes are the most important vectors of infectious diseases and their bites are related to several adverse skin reactions. Permethrin impregnated clothes are an efficient strategy against arthropods' bites; however, its topical efficacy as a repellent has not been well established. We studied the response to permethrin lotion 5 percent and N,N-Diethyl-meta-toluamide (DEET) spray 50 percent applied to the unprotected forearms of 10 volunteers. Each arm was exposed to 20 female mosquitoes of Aedes aegypti. We performed 71 bilateral comparative measurements evaluating the timing for the first bites. The average times for the arm without the product, with permethrin 5 percent, and with DEET 50 percent were: 7.9 seconds, 336.2 seconds and 7512.1 seconds. The results showed a significant difference between repellency times between either product and unprotected controls. In addition, there was a significant difference in time to first bite between permethrin and DEET treated arms (pAedes aegypti bites in this experimental setting. However, permethrin's profile of repellency was significantly inferior to that of DEET.

  17. O Carnaval é um Palco, A Ilha uma Festa: Da performance cultural à exposição da sua metaperformance videográfica

    Directory of Open Access Journals (Sweden)

    Rui Mourão

    2015-11-01

    Full Text Available Neste artigo aborda-se o património cultural imaterial como performance e as suas representações em museus etnográficos como metaperformance, dando especial enfoque à necessidade do uso de imagens em movimento, segundo práticas videográficas, simultaneamente artísticas e documentais, para apresentação expositiva de conteúdos antropológicos. Inicialmente apresenta-se uma argumentação mais teórica sobre a importância do recurso a materiais visuais para representar elementos culturais igualmente visuais, particularmente quando se trata de linguagem não-verbal. Denotativamente, essa reflexão é aplicada no caso da exposição O Carnaval é um Palco, A Ilha uma Festa, que esteve patente no Museu Nacional de Etnologia, em Lisboa, no Museu de Angra do Heroísmo e no Espaço do Conhecimento – Museu da Universidade Federal de Minas Gerais, em Belo Horizonte, Brasil. Aí são retratadas as danças de Carnaval da ilha Terceira a partir de uma videoinstalação multicanal com várias projeções.

  18. Spatial distribution by Canistropsis microps (E. Morren ex Mez Leme (Bromeliaceae: Bromelioideae in the Atlantic rain forest in Ilha Grande, Southeastern Brazil

    Directory of Open Access Journals (Sweden)

    AF. Nunes-Freitas

    Full Text Available Canistropsis microps (Bromeliaceae: Bromelioideae is an endemic species of Atlantic rain forest areas in Rio de Janeiro State, which are very abundant in not very disturbed forests in Ilha Grande, on the southern coast of the State. In this study, we analyzed the vertical and horizontal distribution patterns of the species in an area of rain forest with little evidence of disturbance at Vila Dois Rios, Ilha Grande, relating the patterns to sunlight in the microhabitat. We also identified the types of substrate used by the species and the rate of asexual reproduction. Canistropsis microps had high densities (estimated at 84,425 rosettes/ha, and has an aggregated distribution (Id = 2.86. About 80% of the rosettes were generated by clonal growth, whereas less than 20% were produced from seedlings. Most of the rosettes were found on straight tree trunks (DBH > 50 cm. There was a significant inverse correlation between the incidence of sunlight in the habitat and the abundance of individuals. Rosettes were found up to a maximum height of 9.5 m, but most occured between 1.5 and 5.5 m, where light varied from 25 to 50 µmol.s-1.m-2. We conclude that vertical and horizontal distribution patterns in C. microps may be partially explained by the occurrence of appropriate substrate, an intensity of sunlight favorable to the development of the species and to a high rate of vegetative reproduction.

  19. Parasitism of Hylodes phyllodes (Anura: Cycloramphidae by Hannemania sp. (Acari: Trombiculidae in an area of Atlantic Forest, Ilha Grande, Southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Hatano F.H.

    2007-06-01

    Full Text Available We studied some parameters of the parasitism by the mite Hannemania sp. on the endemic frog Hylodes phyllodes in the Atlantic Forest of Ilha Grande (Rio de Janeiro State, Brazil. Prevalence, mean abundance, mean intensity and total intensity of infestation, body regions infected, and host sexual differences in parasitism rate of the larvae of Hannemania sp. on individuals of H. phyllodes were assessed. Prevalence was 86.5% (N = 37; total of 1,745 larvae of Hannemania sp for male hosts and 91.7% (N =12; total of 330 larvae for female hosts, with no significant difference between the sexes. Overall prevalence of Hannemania sp. on H. phyllodes was 87.7%. Mean intensity of infestation in males (54.5 ± 42.5; range 1-173 larvae was higher than in females (29.9 ± 47.6; range 1-166. We conclude that the rates of intensity, abundance, and prevalence of Hannemania larvae parasitizing Hylodes phyllodes at Ilha Grande were considerably high, suggesting that this species of anuran constitutes a relevant host for this mite species to complete its lifecycle in the area. Differences between males and females in infestation rates probably reflect their differential use of space in the forest.

  20. VECTOR RESISTANCE STATUS OF DENGUE HEMORRHAGIC FEVER (Aedes aegypti IN THE SIDOREJO DISTRICT SALATIGA CITY AGAINST TEMEPHOS (ORGANOPHOSPHATES

    Directory of Open Access Journals (Sweden)

    Ary Oktsari Yanti S

    2014-06-01

    Full Text Available One of the efforts to control the incidence of Dengue Hemorrhagic Fever (DHF is contrled thedengue vector larvae using larvasida. The most widely larvasida used to control larvae Ae.aegypti is temephos. In Indonesia 1% temephos (abate 1SG has been used since 1976, and since1980 has been used for the eradication program ofAe. aegypti larvae. The purpose of this studyis to determine the resistance status of vectors of dengue hemorrhagic fever (Ae. aegypti ofendemic, sporadic, and potentially in Sub District Sidorejo Salatiga City to temephos(organofosfat. This research was conducted using experimental research design (TrueExperiment, posttcst design with control groups (posttest-only Control Group Design. Thepopulation of the research were larvae of Ae. aegypti collected from the study area. Samples testlarvae were used of Ae. aegypti third and early fourth instars larvae which were maintenance ofthe first generation. The result showed that the mortality percentages of Ae. aegypti larvaeof endemic, sporadic and potential administratives against temephos using WHO standardconcentration (0,625; 0,125; 0,025 mg/1 indicates the mortality of Ae. aegypti larvae by 100%Based on the status resistance criteria, Ae. aegypti larvae from endemic, sporadic, and potentialadministratives of Sidorejo Sub-District, Salatiga City is still susceptible to temephos.Keywords : Status of resistance, Aedes aegypti. TemephosSalah satu upaya menurunkan Demam Berdarah Dengue (DBD adalah melaluipengcndalian jentik vektor DBD dengan larvasida. Larvasida yang digunakan untukmengcndalikan jentik Ae. aegypti adalah temephos. Temephos 1% (abate ISG sudah programdi Indonesia sejak 1976, scjak 1980 telah digunakan secara massal untuk programpemberantasan jentik Ae. aegypti. Tujuan penelitian ini adalah untuk mengetahui status resistensivektor demam berdarah dengue Ae. aegypti di kclurahan endemis, sporadis, dan potensialKecamatan Sidorejo Kota Salatiga terhadap temephos

  1. Calculating the survival rate and estimated population density of gravid Aedes aegypti (Diptera, Culicidae in Rio de Janeiro, Brazil Determinação da taxa de sobrevivência e estimativa da densidade populacional de fêmeas grávidas de Aedes aegypti (Diptera, Culicidae no Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Rafael Maciel-de-Freitas

    2008-12-01

    Full Text Available Population size and daily survival rates of disease vectors are important determinants of vectorial capacity. A mark-release-recapture experiment was conducted in a dengue endemic urban neighborhood of Rio de Janeiro, Brazil, to estimate population size, survival rate and vectorial capacity of Aedes aegypti females using back-pack aspirators and gravid sticky traps (MosquiTRAP. Estimations of the gravid female population size were different when using data gathered from just the MosquiTRAP (3,505 individuals or aspirator (1,470. However Ae. aegypti survival rates and longevity were similar irrespective of the method of capture. Up to 26.3% of released females would be able to survive for more than 10 days, the length of time of the extrinsic incubation period. Vectorial capacity value ranged between 0.01567 and 0.4215 and the basic reproductive number (R0 was estimated to be between 0.0695 and 1.88.O tamanho populacional e a taxa de sobrevivência de vetores de doenças são importantes componentes da capacidade vetorial. Um experimento de marcação-soltura-recaptura foi conduzido numa área urbana endêmica de dengue no Rio de Janeiro, Brasil, para estimar o tamanho populacional, taxa de sobrevivência diária e capacidade vetorial de fêmeas de Aedes aegypti; para tal, usaram-se aspirador costal e armadilha para fêmeas grávidas de Ae. aegypti (MosquiTRAP. Estimativas do tamanho populacional de fêmeas grávidas foram diferentes quando foram analisados dados coletados apenas na MosquiTRAP (3.505 indivíduos e aspiradores (1.470. Por outro lado, taxas de sobrevivência e longevidade de Ae. aegypti foram semelhantes independentemente do método de captura empregado. Até 26,3% das fêmeas seriam capazes de sobreviver mais do que dez dias, tempo equivalente à duração do período de incubação extrínseco. O cálculo da capacidade vetorial revelou valores entre 0,01567 e 0,4215, e o número básico de reprodução foi estimado entre 0,0695 e

  2. Efeito larvicida de óleos essenciais de plantas medicinais sobre larvas de Aedes aegypti L. (Diptera:Culicidae

    Directory of Open Access Journals (Sweden)

    Toshik Iarley da Silva

    2017-06-01

    Full Text Available O Aedes aegypti L. é o mosquito mais relevante em termos de inseto de importância para a saúde pública no mundo, pois é transmissor de vírus que causam várias doenças, dentre elas a dengue. Na procura de larvicidas alternativos, os óleos essenciais extraídos de plantas medicinais têm demonstrado alta eficiência. Com isso, objetivou-se com avaliar o potencial larvicida de óleos essenciais, extraídos de espécies medicinais, sobre larvas de A. aegypti. O experimento foi realizado nos Laboratórios de Tecnologia de Produtos e de Entomologia Agrícola da Universidade Federal do Cariri (UFCA, na cidade de Crato, Ceará. Os óleos essenciais de alfazema (Hyptis suaveolens; gonçalo-alves (Astronium fraxinifolium0; alecrim de tabuleiro (Lippia Microphylla; mussambê (Cleome spinosa; marmeleiro (Croton sonderianus; aroeira (Myracrodruon urundeuva; velame (Croton heliotropiifolius e candeeiro (Vanillosmopsis arborea foram extraídos pelo método de hidrodestilação em aparelho tipo Clevenger. Os óleos foram emulsionados com Dimetilsulfóxido 2% (DMSO e diluídos para a concentração de 100 ppm (partes por milhão, utilizando-se o delineamento inteiramente casualisado com nove tratamentos (consistindo nas soluções de 100 ppm de cada óleo mais a solução controle com água e DMSO e quatro repetições, utilizando-se dez larvas para cada tratamento, sendo avaliado o número de larvas mortas. Observou-se que todos os óleos essenciais apresentaram efeito larvicida, porém os de candeeiro e de alfazema foram os que se destacaram.Abstract: Aedes aegypti L. is the most relevant mosquito in terms of public health importance of the world, because it’s the virus transmitter that causes many diseases, among this dengue. Looking for alternatives larvicides, the essential oils extracted from medicinal plants have demonstrated high efficiency. Thereby, it was objectified in this work to evaluate the larvicide potential of some essential oils

  3. Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology

    Czech Academy of Sciences Publication Activity Database

    Bizzarro, B.; Barros, M.S.; Maciel, C.; Gueroni, D.I.; Lino, C.N.; Campopiano, J.; Kotsyfakis, Michalis; Amarante-Mendes, G.P.; Calvo, E.; Capurro, M.L.; Sa-Nunes, A.

    2013-01-01

    Roč. 6, NOV 2013 (2013), s. 329 ISSN 1756-3305 Institutional support: RVO:60077344 Keywords : dendritic cells * T-cells * Aedes aegypti * saliva * apoptosis Subject RIV: EC - Immunology Impact factor: 3.251, year: 2013

  4. Global dynamics of a PDE model for aedes aegypti mosquitoe incorporating female sexual preference

    KAUST Repository

    Parshad, Rana; Agusto, Folashade B.

    2011-01-01

    In this paper we study the long time dynamics of a reaction diffusion system, describing the spread of Aedes aegypti mosquitoes, which are the primary cause of dengue infection. The system incorporates a control attempt via the sterile insect

  5. Susceptibility status of Aedes aegypti (L.) (Diptera: Culicidae) to temephos in Venezuela.

    Science.gov (United States)

    Alvarez, Leslie C; Ponce, Gustavo; Oviedo, Milagros; Lopez, Beatriz; Flores, Adriana E

    2014-08-01

    Temephos is an insecticide widely used in Venezuela to control the proliferation of the larvae of Aedes aegypti (L.), the principal vector of dengue virus. The aim of this study was to identify the susceptibility to temephos of Ae. aegypti in four locations in western Venezuela: Lara, Tres Esquinas, Ureña and Pampanito. Larval bioassays were conducted on samples collected in 2008 and 2010, and the levels of α- and β-esterases, mixed-function oxidases, glutathione-S-transferase and insensitive acethyl cholinesterase were determined. Larval populations from western Venezuela obtained during 2008 and 2010 were found to be susceptible to temephos, with low resistance ratios and without overexpression of enzymes. The low RR values reveal the effectiveness of temephos in controlling the larval populations of Ae. aegypti. Control strategies must be vigorously monitored to maintain the susceptibility to temephos of these populations of Ae. aegypti. © 2013 Society of Chemical Industry.

  6. Community-based control of Aedes aegypti by adoption of eco ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    empower women and student communities to estab- lish a sustainable nature ... Control of Aedes aegypti by eco-health methods in Chennai. Pathogens and Global ... predominantly middle class with good/satisfactory housing — often of two ...

  7. Mosquito adulticidal properties of Delonix elata (Family: Fabaceae against dengue vector, Aedes aegypti (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Mohan Rajeswary

    2014-05-01

    Full Text Available Objective: To determine the adulticidal activity of hexane, benzene, chloroform, ethyl acetate and methanol leaf and seed extracts of Delonix elata (D. elata against Aedes aegypti (Ae. aegypti. Methods: The bioassay was conducted in an experimental kit consisting of two cylindrical plastic tubes both measuring 125 mm×44 mm following the WHO method; mortality of the mosquitoes was recorded after 24 h. Results: The adulticidal activity of plant leaf and seed extracts showed moderate toxic effect on the adult mosquitoes after 24 h of exposure period. However, the highest adulticidal activity was observed in the leaf methanol extract of D. elata against Ae. aegypti with the LC50 and LC90 values 162.87 and 309.32 mg/L, respectively. Conclusions: From this result, it can be concluded the crude extract of D. elata was an excellent potential for controlling Ae. aegypti mosquitoes.

  8. Monooxygenase activitity in Aedes aegypti population in Tembalang subdistrict, Semarang city

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    Dyah Widiastuti

    2015-06-01

    Full Text Available Dengue Haemorrhagic Fever (DHF is a major health problem in Tembalang sub district, Semarang City. Fogging with insecticide applications was done frequently as an effort to control Dengue vectors. The use of insecticides from the same class in a long time can lead to resistance in mosquitos’ population. The research aimed to observe the activity of monooxygenases in Aedes aegypti populations in Tembalang Subdistrict, Semarang. The study was conducted during February-November 2014 with a cross-sectional design in 10 villages in Tembalang Subdistirict, Semarang City. Field strains of Ae. aegypti eggs were collected using ovitraps. The collected eggs were grown under standard condition to adult mosquitoes. Mosquitos’ homogenate were stored at -85C and used for biochemical assays. The results showed there was increased monooxygenases activity in Ae. aegypti populations. Resistance to synthetic pyrethroid insecticide in Ae. aegypti mosquitoes population in Tembalang Subdistrict might be caused by the mechanism of detoxification enzymes in particular monooxygenases

  9. Estimativa das máximas pistas de vento no reservatório da barragem de Ilha Solteira, Estado de São Paulo - DOI: 10.4025/actascitechnol.v29i1.112

    Directory of Open Access Journals (Sweden)

    Marcelo Marques

    2007-12-01

    Full Text Available Grande parte dos métodos empregados na estimativa da altura de ondas, geradas pela ação do vento, necessita da determinação do comprimento da pista sobre a qual o vento atua. A escolha do método depende da natureza do corpo de água, podendo ser aplicável a áreas oceânicas ou a corpos d’água interiores, como baías, lagos e reservatórios. Uma das soluções rotineiras tem sido a aplicação direta do método validado em águas oceânicas para águas interiores sem levar, portanto, em consideração, o efeito de restrição oferecido pelas margens. Não obstante, a excessiva quantidade de operações do método aplicado a corpos de água interiores (onde a margem é condição de restrição, pode, se não usado uma base gráfica suficientemente precisa da margem acrescido da subjetividade do operador, não assegurar um grau de precisão satisfatório. Assim exposto, esta comunicação científica traz à luz esta discussão, propondo uma solução clássica baseada na aplicação do método adequado a águas interiores (Saville et al., 1954, via processamento automatizado. Para tanto, foi desenvolvido um programa em linguagem computacional AutoLISP. A aplicação do programa determinou as máximas pistas de vento no reservatório da barragem de Ilha Solteira, Estado de São Paulo, como estando entre 9,5 e 12,5 km, contrariamente a um estudo anterior que havia previsto pistas bem superiores (fator da ordem de 3.

  10. Insecticide resistance, associated mechanisms and fitness aspects in two Brazilian Stegomyia aegypti (= Aedes aegypti) populations.

    Science.gov (United States)

    Viana-Medeiros, P F; Bellinato, D F; Martins, A J; Valle, D

    2017-12-01

    In Brazil, insecticide resistance in Stegomyia aegypti (= Aedes aegypti) (Diptera: Culicidae) populations to pyrethroids and to the organophosphate (OP) temephos is disseminated. Currently, insect growth regulators (IGRs) and the OP malathion are employed against larvae and adults, respectively. Bioassays with mosquitoes from two northeast municipalities, Crato and Aracaju, revealed, in both populations, susceptibility to IGRs and malathion (RR 95  ≤ 2.0), confirming the effectiveness of these compounds. By contrast, temephos and deltamethrin (pyrethroid) resistance levels were high (RR 95  > 10), which is consistent with the use of intense chemical control. In Crato, RR 95 values were > 50 for both compounds. Knock-down-resistant (kdr) mutants in the voltage-gated sodium channel, the pyrethroid target site, were found in 43 and 32%, respectively, of Aracaju and Crato mosquitoes. Biochemical assays revealed higher metabolic resistance activity (esterases, mixed function oxidases and glutathione-S-transferases) at Aracaju. With respect to fitness aspects, mating effectiveness was equivalently impaired in both populations, but Aracaju mosquitoes showed more damaging effects in terms of longer larval development, decreased bloodmeal acceptance, reduced engorgement and lower numbers of eggs laid per female. Compared with mosquitoes in Crato, Aracaju mosquitoes exhibited lower OP and pyrethroid RR 95 , increased activity of detoxifying enzymes and greater effect on fitness. The potential relationship between insecticide resistance mechanisms and mosquito viability is discussed. © 2017 The Authors. Medical and Veterinary Entomology published by John Wiley & Sons Ltd on behalf of The Royal Entomological Society.

  11. Programa de computador para reconhecimento da larva de Aedes aegypti e Aedes albopictus

    OpenAIRE

    São Thiago, André Iwersen de; Kupek, Emil; Ferreira Neto, Joaquim Alves; São Thiago, Paulo de Tarso

    2002-01-01

    Software for pattern recognition of the larvae of mosquitoes Aedes aegypti and Aedes albopictus, biological vectors of dengue and yellow fever, has been developed. Rapid field identification of larva using a digital camera linked to a laptop computer equipped with this software may greatly help prevention campaigns.Foi desenvolvido um programa de computador para reconhecimento da larva de Aedes aegypti e Aedes albopictus, vetores biológicos de dengue e febre amarela. O programa possibilita rá...

  12. Wolbachia Blocks Currently Circulating Zika Virus Isolates in Brazilian Aedes aegypti Mosquitoes

    OpenAIRE

    Dutra, Heverton Leandro Carneiro; Rocha, Marcele Neves; Dias, Fernando Braga Stehling; Mansur, Simone Brutman; Caragata, Eric Pearce; Moreira, Luciano Andrade

    2016-01-01

    Summary The recent association of Zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the Zika vector, Aedes aegypti mosquitoes. Wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. Here we report that Aedes aegypti harboring Wolbachia are highly resistant to infection with two currently circulating Zika virus isolates from the recent Brazilian epide...

  13. Contact Irritant Responses of Aedes aegypti Using Sublethal Concentration and Focal Application of Pyrethroid Chemicals

    OpenAIRE

    Manda, Hortance; Shah, Pankhil; Polsomboon, Suppaluck; Chareonviriyaphap, Theeraphap; Castro-Llanos, Fanny; Morrison, Amy; Burrus, Roxanne G.; Grieco, John P.; Achee, Nicole L.

    2013-01-01

    BACKGROUND: Previous studies have demonstrated contact irritant and spatial repellent behaviors in Aedes aegypti following exposure to sublethal concentrations of chemicals. These sublethal actions are currently being evaluated in the development of a push-pull strategy for Ae. aegypti control. This study reports on mosquito escape responses after exposure to candidate chemicals for a contact irritant focused push-pull strategy using varying concentrations and focal application. METHODS: Cont...

  14. Aktivitas enzim monooksigenase pada populasi nyamuk Aedes aegypti di Kecamatan Tembalang, Kota Semarang

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    Dyah Widiastuti

    2015-06-01

    Full Text Available Abstract. Dengue Haemorrhagic Fever (DHF is a major health problem in Tembalang sub district, Semarang City. Fogging with insecticide applications was done frequently as an effort to control Dengue vectors. The use of insecticides from the same class in a long time can lead to resistance in mosquitos’ population. The research aimed to observe the activity of monooxygenases in Aedes aegypti populations in Tembalang Subdistrict, Semarang. The study was conducted during February-November 2014 with a cross-sectional design in 10 villages in Tembalang Subdistirict, Semarang City. Field strains of Ae. aegypti eggs were collected using ovitraps. The collected eggs were grown under standard condition to adult mosquitoes. Mosquitos’ homogenate were stored at -85°C and used for biochemical assays. The results showed there was increased monooxygenases activity in Ae. aegypti populations. Resistance to synthetic pyrethroid insecticide in Ae. aegypti mosquitoes population in Tembalang Subdistrict might be caused by the mechanism of detoxification enzymes in particular monooxygenases Keywords: monooxygenase, insecticide, Ae. aegypti, resistance  Abstrak. Demam Berdarah Dengue (DBD masih menjadi masalah kesehatan utama di Kecamatan Tembalang, Kota Semarang. Tindakan fogging untuk pengendalian vektor DBD sering dilakukan. Penggunaan insektisida dari golongan yang sama dalam waktu cukup lama dapat memicu terjadinya resistensi. Tujuan penelitian untuk mengamati aktivitas enzim monooksigenase pada populasi nyamuk Aedes aegypti di Kecamatan Tembalang, Kota Semarang. Penelitian dilaksanakan bulan Februari-November 2014 dengan desain potong lintang di 10 desa di Kecamatan Tembalang, Kota Semarang. Pada penelitian ini dilakukan pemasangan ovitrap untuk mendapatkan sampel telur yang dipelihara menjadi nyamuk dewasa. Sampel homogenate nyamuk disimpan pada suhu -85°C, selanjutnya dilakukan peng-ujian resistensi dengan uji biokimia untuk melihat aktivitas enzim

  15. Evaluation of Three Commercial Backpack Sprayers with Aqualuer (registered trademark) 20-20 Against Caged Adult Aedes aegypti

    Science.gov (United States)

    2016-03-11

    Sprayers with Aqualuer® 20–20 Against Caged Adult Aedes Aegypti Author(s): Derrick Conover, Ali Fulcher, Michael L. Smith, Muhammad Farooq, Marcia K... AEDES AEGYPTI DERRICK CONOVER,1 ALI FULCHER,1 MICHAEL L. SMITH,1 MUHAMMAD FAROOQ,2 MARCIA K. GAINES1 AND RUI-DE XUE1,3 ABSTRACT. Three commercially...adult Aedes aegypti in semifield trials in northeastern Florida. Two battery-powered sprayers, Birchmeier and Hudson, were compared with the standard

  16. The Effects of Pre-Exposure to DEET on the Downstream Blood-Feeding Behaviors of Aedes aegypti Mosquitoes

    Science.gov (United States)

    2016-06-10

    1 Sugiharto et al.: Aedes aegypti blood- Michael J. Turell 1 feeding after DEET pre-exposure Virology Division, USAMRIID 2 1425 Porter...Pre-exposure to DEET on the Downstream Blood-Feeding Behaviors of 9 Aedes aegypti Mosquitoes1 10 11 Victor A. Sugiharto,2 John P. Grieco,2,3...insect behavior for disease prevention. However, genetic insensitivity and 31 habituation in Aedes aegypti (L.) mosquitoes after pre-exposure to DEET

  17. Investigating Potential Effects of Dengue Virus Infection and Pre-exposure to DEET on Aedes aegypti Behaviors

    Science.gov (United States)

    2016-02-05

    Investigating Potential Effects of Dengue Virus Infection and Pre-exposure to DEET on Aedes aegypti Behaviors by Victor A...exposure to DEET on Aedes aegypti Behaviors" Name of Candidate: Victor Sugiharto Doctor of Philosophy Degree February 5, 2016 DISSERTATION AND...Infection and Pre-exposure to DEET on Aedes aegypti Behaviors" is appropriately acknowledged and. beyond brief excerpts , is with the permission of

  18. Adulticidal properties of Pithecellobium dulce (Roxb.) Benth. (Family: Fabaceae) against dengue vector, Aedes aegypti (Linn.) (Diptera: Culicidae)

    OpenAIRE

    Mohan Rajeswary; Marimuthu Govindarajan

    2014-01-01

    Objective: To examine the toxicity of mosquito adulticidal activity of different solvent leaf and seed extracts of Pithecellobium dulce (P. dulce) against dengue vector, Aedes aegypti (Ae. aegypti). Methods: Adulticidal efficacy of the crude leaf and seed extracts of P. dulce with five different solvents like benzene, hexane, ethyl acetate, methanol and chloroform was tested against the five to six day old adult female mosquitoes of Ae. aegypti. The adult mortality was observed...

  19. OVIPOSITION PREFERENCE OF Aedes aegypti AGAINST VARIOUS LEAF EXTRACT AS AN ATRACTANT

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    Sara Gustia Wibowo

    2015-07-01

    Full Text Available Aedes aegypti merupakan vektor pembawa virus Dengue yang menyebabkan penyakit Demam Berdarah Dengue (DBD.Pengendalian secara alami menggunakan bahan nabati merupakan alternatif pengendalian yang ramah lingkungan. Salahsatu pengendalian secara alami adalah memodifikasi ovitrap dengan penambahan zat aktif nabati sebagai atraktan untukmenarik nyamuk bertelur dan dapat menjadi ovisida dan larvasida. Tujuan penelitian ini adalah mengetahui preferensibertelurnya nyamuk Ae. aegypti pada ovitrap dengan ekstrak daun mimba (Azadirachta indica, kecubung (Datura metel,zodia (Evodia suavolens dan jenu (Derris elliptica. Jenis penelitian eksperimen dengan rancangan acak lengkap. Keempatjenis ekstrak daun tersebut diisiikan pada ovitrap, dimasukan ke dalam kandang yang berisi 30 ekor Ae. aegypti dengankondisi kenyang darah. Pengamatan dilakukan setiap hari sampai hari ke-3. Hasil uji preferensi berbagai jenis ekstrak inimenunjukkan ovitrap yang berisi ekstrak daun jenu (D. elliptica lebih banyak ditemukan telur Ae. aegypti dibandingkandengan kontrol maupun ovitrap dengan ekstrak daun lainnya. Persentase telur pada kontainer dengan ekstrak jenu adalah44,2%, sedangkan yang terkecil ekstrak zodia (E. suaveolans 9,2%. Hasil uji Anova menunjukkan bahwa perlakuan antarkelompok berbeda nyata (p=0,000. Tanaman jenu (D. elliptica mempunyai potensi sebagai atraktan terhadap nyamuk Ae.aegypti dalam proses oviposisi.Kata kunci: atraktan, oviposisi, jenu (Derris elliptica, Aedes aegypti

  20. Aedes aegypti resistance development to commonly used insecticides in Jakarta, Indonesia.

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    Penny Humaidah Hamid

    Full Text Available Aedes aegypti is the primary vector of various relevant arthropod-borne viral infectious diseases worldwide. The mosquito control is still mainly performed by using insecticides but their effectiveness is increasingly questioned nowadays. We here conducted a study on Ae. aegypti resistance development towards several commonly used insecticides in the capital city of Jakarta, Indonesia. In order to achieve this goal, Ae. aegypti eggs from Jakarta were collected with ovitraps and hatched in the insectary of the Gadjah Mada University, Indonesia. The F0 generations were used for WHO resistance tests and knockdown resistance (kdr assays. Presented results clearly showed that there was resistance development of Ae. aegypti populations to the here tested pyrethroid insecticides (i. e. permethrin. Observed mortalities were less than 90% with highest resistance against 0.75% permethrin concentrations. Furthermore, a significant association of V1016G gene mutations with resistance phenotypes to 0.75% permethrin was observed. Nevertheless, the F1534C mutation did not show a significant correlation to resistance development. In conclusion, our results show that populations of Ae. aegypti mosquitoes within the city of Jakarta have developed resistance against several routinely used pyrethroid insecticides in local performed control programs. Thus, the regular verification/assessment of resistance development status will hopefully help in the future to assist local public health authorities in their mosquito control programs by recommending and managing the rotation of different routinely used insecticides with diverse effector mechanisms in order to delay Ae. aegypti resistance development.

  1. The resistance map of Aedes aegypti (Linn. to cypermethrin and malathion in Central Java

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    Bina Ikawati

    2015-06-01

    Full Text Available The increasing prevalence of Dengue Haemmorhaegic Fever (DHF is spread through all districts in Indonesia. Dengue Haemorrhagic Fever Control such as vector control, focussing to break DHF transmission. Some research about Ae. aegypti resistance had been done in DHF endemic area in Central Java. Resistance status of Ae. aegypti against insecticide programme promoted by health government in middle and low endemic DHF in Central Java was investigated in this research. Sample collected from 100 houses selected purposively in every village, at every District there were 3 villages selected. Samples consisted of egg, larvae and adult mosquitoes of Ae. aegypti, and reared to get F1. Resistance test of Ae. aegypti done by using WHO susceptibility impregnated paper test procedure. This research showed that Ae. aegypti in all research location had been resistance to malathion 0.8% with mosquitoes mortality average between 13.80%-61.67% and almost all sample is resistance to cypermethrin 0.05% with mosquitoes mortality between 10.00%-63.33% except with sample from Banjarnegara District which has mosquitoes mortality of 84.20%. The conclusion of this research is that Ae. aegypti in all research location had been resistance to malathion. Almost all location resistant to cypermethrin except Banjarnegara District sample which has tolerance level.

  2. Species Distribution Modelling of Aedes aegypti in two dengue-endemic regions of Pakistan.

    Science.gov (United States)

    Fatima, Syeda Hira; Atif, Salman; Rasheed, Syed Basit; Zaidi, Farrah; Hussain, Ejaz

    2016-03-01

    Statistical tools are effectively used to determine the distribution of mosquitoes and to make ecological inferences about the vector-borne disease dynamics. In this study, we utilised species distribution models to understand spatial patterns of Aedes aegypti in two dengue-prevalent regions of Pakistan, Lahore and Swat. Species distribution models can potentially indicate the probability of suitability of Ae. aegypti once introduced to new regions like Swat, where invasion of this species is a recent phenomenon. The distribution of Ae. aegypti was determined by applying the MaxEnt algorithm on a set of potential environmental factors and species sample records. The ecological dependency of species on each environmental variable was analysed using response curves. We quantified the statistical performance of the models based on accuracy assessment and spatial predictions. Our results suggest that Ae. aegypti is widely distributed in Lahore. Human population density and urban infrastructure are primarily responsible for greater probability of mosquito occurrence in this region. In Swat, Ae. aegypti has clumped distribution, where urban patches provide refuge to the species in an otherwise hostile heterogeneous environment and road networks are assumed to have facilitated in passive-mediated dispersal of species. In Pakistan, Ae. aegypti is expanding its range northwards; this could be associated with rapid urbanisation, trade and travel. The main implication of this expansion is that more people are at risk of dengue fever in the northern highlands of Pakistan. © 2016 John Wiley & Sons Ltd.

  3. THE INSECT GROWTH REGULATOR, TRIFLUMURON (OMS-2015 AGAINST AEDES AEGYPTI IN JAKARTA, INDONESIA

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    M. Soekirno

    2012-09-01

    Full Text Available Uji laboratorium dan lapangan dengan IGR Triflumuron (OMS-2015 terhadap larva nyamuk Aedes aegypti telah dilakukan di Jakarta. Uji laboratorium dilakukan dengan 6 variasi dosis, yaitu 0,004; 0,011; 0,034; 0,10; 0,33 dan 1,0 ppm Triflumuron terhadap perkembangan larva nyamuk Ae. aegypti di dalam tempayan. Dari uji laboratorium dapat diketahui bahwa Triflumuron dengan dosis 0,004 ppm dapat menekan perkembangan pupa untuk menjadi dewasa dalam waktu 2 minggu, sedangkan dosis 0,10 ppm menekan padat populasi nyamuk Ae. aegypti selama 4 minggu dan dosis 1,0 ppm menekan padat populasi nyamuk Ae. aegypti selama 8 minggu. Uji lapangan dengan menggunakan Triflumuron di daerah pelabuhan Tanjung Priok, Jakarta, seluas 27 hektar dengan dua kali perlakuan, dengan dosis 0,042 dan 0,075 ppm, terjadi penurunan populasi nyamuk Ae. aegypti dewasa dan indeks pupa menjadi 0 dalam 4 hari setelah perlakuan. Penurunan populasi nyamuk Ae. aegypti dewasa terlihat setelah 2 minggu se­sudah perlakuan dengan tidak berhasilnya larva/pupa menjadi nyamuk dewasa. 

  4. Molecular characterization of Aedes aegypti (L. (Diptera: Culicidae of Easter Island based on analysis of the mitochondrial ND4 gene

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    Claudia Andrea Núñez

    2016-06-01

    Full Text Available ABSTRACT Aedes aegypti mosquitoes are the main vector of viruses Dengue, Zika and Chikungunya. Shortly after the first report of the dengue vector Ae. aegypti in Easter Island (Rapa Nui in late 2000, the first disease outbreak dengue occurred. Viral serotyping during the 2002 outbreak revealed a close relationship with Pacific DENV-1 genotype IV viruses, supporting the idea that the virus most likely originated in Tahiti. Mitochondrial NADH dehydrogenase subunit 4 (ND4 DNA sequences generated from 68 specimens of Ae. aegypti from Easter Island reporting a unique finding of a single maternal lineage of Ae. aegypti on Easter Island.

  5. Principales criaderos para Aedes aegypti y culícidos asociados, Argentina Main breeding-containers for Aedes aegypti and associated culicids, Argentina

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    Marina Stein

    2002-10-01

    Full Text Available Se identificaron criaderos de Aedes (Stegomyia aegypti en dos ciudades de la provincia del Chaco (Noreste de Argentina: Presidencia Roque Saenz Peña y Machagai. Los recipientes encontrados en los patios de las viviendas capaces de colectar agua se clasificaron según tipo y tamaño, se contaron y examinaron. Aedes aegypti y Culex quinquefasciatus fueron las especies mas abundantes, encontrándose además Cx. maxi, Cx. saltanensis y Ochlerotatus scapularis. Los neumáticos y cajas de baterías para autos fueron los recipientes que más aportaron a la producción de formas inmaduras de los culícidos colectados. Las lluvias fueron un factor importante para la proliferacion de Ae. aegypti, así como también el habito comun en la población de guardar recipientes en sus casas que permitan el desarrollo de estos culícidos.Breeding containers for Aedes (Stegomyia aegypti were identified in two cities of Chaco Province (northeast Argentina: Presidencia Roque Saenz Peña and Machagai. All water-retaining recipients found in house backyards capable to retain water were classified according to their type and size, counted and checked. Aedes aegypti and Culex quinquefasciatus were the most frequently collected species, being also found Cx. maxi, Cx. saltanensis and Ochlerotatus scapularis. Tires and car batteries represented the most important type of container where immature forms of culicids could be found. Rain was an important factor for Ae. aegypti proliferation, as well as the widespread habit of the population of keeping useless containers at home, which allows the development of culicids.

  6. Larvicidal activity of some Cerrado plant extracts against Aedes aegypti.

    Science.gov (United States)

    Rodrigues, A M S; De Paula, J E; Degallier, N; Molez, J E; Espindola, L S

    2006-06-01

    One hundred ninety hexanic and ethanolic extract from 27 plant species from the Cerrado biome of Brazil were tested for larvicidal activity against 3rd-stage Aedes aegypti larvae at 500 microg/ml. Fourteen extracts from 7 species showed activity (>65% mortality) against the larvae. Of these Dugeutia furfuracea, Piptocarpha rotundifolia, Casearia sylvestris var. lingua, Serjania lethalis, and Xylopia aromatica were active at 56.6, 162.31, 232.4, 285.76, and 384.37 microg/ml, respectively. Annona crassiflora and Cybistax antisyphilitica showed activity at 23.06 and 27.61 microg/ml. The larvicidal properties of these species are described for the first time, and may prove to be promising in active chemical compound isolation.

  7. Developmental neurogenetics of sexual dimorphism in Aedes aegypti.

    Science.gov (United States)

    Duman-Scheel, Molly; Syed, Zainulabeuddin

    Sexual dimorphism, a poorly understood but crucial aspect of vector mosquito biology, encompasses sex-specific physical, physiological, and behavioral traits related to mosquito reproduction. The study of mosquito sexual dimorphism has largely focused on analysis of the differences between adult female and male mosquitoes, particularly with respect to sex-specific behaviors related to disease transmission. However, sexually dimorphic behaviors are the products of differential gene expression that initiates during development and therefore must also be studied during development. Recent technical advancements are facilitating functional genetic studies in the dengue vector Aedes aegypti, an emerging model for mosquito development. These methodologies, many of which could be extended to other non-model insect species, are facilitating analysis of the development of sexual dimorphism in neural tissues, particularly the olfactory system. These studies are providing insight into the neurodevelopmental genetic basis for sexual dimorphism in vector mosquitoes.

  8. Developmental neurogenetics of sexual dimorphism in Aedes aegypti

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    Molly eDuman-Scheel

    2015-06-01

    Full Text Available Sexual dimorphism, a poorly understood but crucial aspect of vector mosquito biology, encompasses sex-specific physical, physiological, and behavioral traits related to mosquito reproduction. The study of mosquito sexual dimorphism has largely focused on analysis of the differences between adult female and male mosquitoes, particularly with respect to sex-specific behaviors related to disease transmission. However, sexually dimorphic behaviors are the products of differential gene expression that initiates during development and therefore must also be studied during development. Recent technical advancements are facilitating functional genetic studies in the dengue vector Aedes aegypti, an emerging model for mosquito development. These methodologies, many of which could be extended to other non-model insect species, are facilitating analysis of the development of sexual dimorphism in neural tissues, particularly the olfactory system. These studies are providing insight into the neurodevelopmental genetic basis for sexual dimorphism in vector mosquitoes.

  9. Repellents inhibit P450 enzymes in Stegomyia (Aedes aegypti.

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    Gloria Isabel Jaramillo Ramirez

    Full Text Available The primary defence against mosquitoes and other disease vectors is often the application of a repellent. Despite their common use, the mechanism(s underlying the activity of repellents is not fully understood, with even the mode of action of DEET having been reported to be via different mechanisms; e.g. interference with olfactory receptor neurones or actively detected by olfactory receptor neurones on the antennae or maxillary palps. In this study, we discuss a novel mechanism for repellence, one of P450 inhibition. Thirteen essential oil extracts from Colombian plants were assayed for potency as P450 inhibitors, using a kinetic fluorometric assay, and for repellency using a modified World Health Organisation Pesticide Evaluations Scheme (WHOPES arm-in cage assay with Stegomyia (Aedes aegypti mosquitoes. Bootstrap analysis on the inhibition analysis revealed a significant correlation between P450-inhibition and repellent activity of the oils.

  10. Resistance of Aedes aegypti to temephos and adaptive disadvantages

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    Morgana Michele Cavalcanti de Souza Leal Diniz

    2014-10-01

    Full Text Available OBJECTIVE To evaluate the resistance of Aedes aegypti to temephos Fersol 1G (temephos 1% w/w associated with the adaptive disadvantage of insect populations in the absence of selection pressure. METHODS A diagnostic dose of 0.28 mg a.i./L and doses between 0.28 mg a.i./L and 1.40 mg a.i./L were used. Vector populations collected between 2007 and 2008 in the city of Campina Grande, state of Paraíba, were evaluated. To evaluate competition in the absence of selection pressure, insect populations with initial frequencies of 20.0%, 40.0%, 60.0%, and 80.0% resistant individuals were produced and subjected to the diagnostic dose for two months. Evaluation of the development of aquatic and adult stages allowed comparison of the life cycles in susceptible and resistant populations and construction of fertility life tables. RESULTS No mortality was observed in Ae. aegypti populations subjected to the diagnostic dose of 0.28 mg a.i./L. The decreased mortality observed in populations containing 20.0%, 40.0%, 60.0%, and 80.0% resistant insects indicates that temephos resistance is unstable in the absence of selection pressure. A comparison of the life cycles indicated differences in the duration and viability of the larval phase, but no differences were observed in embryo development, sex ratio, adult longevity, and number of eggs per female. CONCLUSIONS The fertility life table results indicated that some populations had reproductive disadvantages compared with the susceptible population in the absence of selection pressure, indicating the presence of a fitness cost in populations resistant to temephos.

  11. Wolbachia-associated bacterial protection in the mosquito Aedes aegypti.

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    Yixin H Ye

    Full Text Available BACKGROUND: Wolbachia infections confer protection for their insect hosts against a range of pathogens including bacteria, viruses, nematodes and the malaria parasite. A single mechanism that might explain this broad-based pathogen protection is immune priming, in which the presence of the symbiont upregulates the basal immune response, preparing the insect to defend against subsequent pathogen infection. A study that compared natural Wolbachia infections in Drosophila melanogaster with the mosquito vector Aedes aegypti artificially transinfected with the same strains has suggested that innate immune priming may only occur in recent host-Wolbachia associations. This same study also revealed that while immune priming may play a role in viral protection it cannot explain the entirety of the effect. METHODOLOGY/FINDINGS: Here we assess whether the level of innate immune priming induced by different Wolbachia strains in A. aegypti is correlated with the degree of protection conferred against bacterial pathogens. We show that Wolbachia strains wMel and wMelPop, currently being tested for field release for dengue biocontrol, differ in their protective abilities. The wMelPop strain provides stronger, more broad-based protection than wMel, and this is likely explained by both the higher induction of immune gene expression and the strain-specific activation of particular genes. We also show that Wolbachia densities themselves decline during pathogen infection, likely as a result of the immune induction. CONCLUSIONS/SIGNIFICANCE: This work shows a correlation between innate immune priming and bacterial protection phenotypes. The ability of the Toll pathway, melanisation and antimicrobial peptides to enhance viral protection or to provide the basis of malaria protection should be further explored in the context of this two-strain comparison. This work raises the questions of whether Wolbachia may improve the ability of wild mosquitoes to survive pathogen

  12. Insecticide resistance status of Aedes aegypti (L.) from Colombia.

    Science.gov (United States)

    Fonseca-González, Idalyd; Quiñones, Martha L; Lenhart, Audrey; Brogdon, William G

    2011-04-01

    To evaluate the insecticide susceptibility status of Aedes aegypti (L.) in Colombia, and as part of the National Network of Insecticide Resistance Surveillance, 12 mosquito populations were assessed for resistance to pyrethroids, organophosphates and DDT. Bioassays were performed using WHO and CDC methodologies. The underlying resistance mechanisms were investigated through biochemical assays and RT-PCR. All mosquito populations were susceptible to malathion, deltamethrin and cyfluthrin, and highly resistant to DDT and etofenprox. Resistance to lambda-cyhalothrin, permethrin and fenitrothion ranged from moderate to high in some populations from Chocó and Putumayo states. In Antioquia state, the Santa Fe population was resistant to fenitrothion. Biochemical assays showed high levels of both cytochrome P450 monooxygenases (CYP) and non-specific esterases (NSE) in some of the fenitrothion- and pyrethroid-resistant populations. All populations showed high levels of glutathione-S-transferase (GST) activity. GSTe2 gene was found overexpressed in DDT-resistant populations compared with Rockefeller susceptible strain. Differences in insecticide resistance status were observed between insecticides and localities. Although the biochemical assay results suggest that CYP and NSE could play an important role in the pyrethroid and fenitrothion resistance detected, other mechanisms remain to be investigated, including knockdown resistance. Resistance to DDT was high in all populations, and GST activity is probably the main enzymatic mechanism associated with this resistance. The results of this study provide baseline data on insecticide resistance in Colombian A. aegypti populations, and will allow comparison of changes in susceptibility status in this vector over time. Copyright © 2011 Society of Chemical Industry.

  13. Larvicidal activity of the water extract of Moringa oleifera seeds against Aedes aegypti and its toxicity upon laboratory animals

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    Paulo M.P. Ferreira

    2009-06-01

    Full Text Available In this work, biological effects of the water extract of Moringa oleifera seeds (WEMOS were assessed on eggs and 3rd instar larvae of Aedes aegypti and on its toxicity upon laboratory animals (Daphnia magna, mice and rats. Crude WEMOS showed a LC50 value of 1260µg/mL, causing 99.2 ± 2.9% larvae mortality within 24 h at 5200µg/mL, though this larvicidal activity has been lost completely at 80ºC/10 min. WEMOS did not demonstrate capacity to prevent egg hatching. After extensive dialyses of the crude WEMOS into watersoluble dialyzable (DF and nondyalizable (NDF fractions, only DF maintained its efficacy to kill larvae. Acute toxicity evaluations on daphnids (EC50 of 188.7µg/mL and mice (LD50 of 446.5 mg/kg body weight pointed out to low toxicity. Despite the thymus hypertrophy, WEMOS revealed to be harmless in orally and subacutelytreated rats. In conclusion, WEMOS has thermostable bioactive compounds against Ae. aegypti larvae with apparent molecular mass lower than 12 kDa and moderately toxic potential.Neste trabalho, o extrato aquoso das sementes de Moringaoleifera (EASMO foi avaliado quanto aos seus efeitos biológicos sobre ovos e larvas de Aedes aegypti no 3ºestágio de desenvolvimento e sua toxicidade sobre animais de laboratório(Daphnia magna, camundongos e ratos. O EASMO bruto revelou uma CL50 de 1.260 µg/mL, causando 99, 2 ± 2, 9% de mortalidade em 24 h na concentração de 5.200 µg/mL, embora o mesmo não tenha sido capaz de impedir a eclosão dos ovos. A atividade larvicida extinguiu-se após aquecimento do extrato a 80ºC/10 min. Diálises sucessivas do EASMO bruto resultaram em duas frações solúveis em água (Fração dializável, FD; Fração nãodializável, FND, dentre as quais apenas a FD mostrou ação larvicida. Testes de toxicidade aguda realizadosem dáfnias (CE50 de 188, 7 µg/mL e camundongos (DL50 de446,5 mg/kg de peso corpóreo evidenciaram baixa toxicidade. Apesar da hipertrofia tímica, o EASMO mostrou ser

  14. Vector Competence of Aedes aegypti and Aedes polynesiensis Populations from French Polynesia for Chikungunya Virus.

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    Vaea Richard

    2016-05-01

    Full Text Available From October 2014 to March 2015, French Polynesia experienced for the first time a chikungunya outbreak. Two Aedes mosquitoes may have contributed to chikungunya virus (CHIKV transmission in French Polynesia: the worldwide distributed Ae. aegypti and the Polynesian islands-endemic Ae. polynesiensis mosquito.To investigate the vector competence of French Polynesian populations of Ae. aegypti and Ae. polynesiensis for CHIKV, mosquitoes were exposed per os at viral titers of 7 logs tissue culture infectious dose 50%. At 2, 6, 9, 14 and 21 days post-infection (dpi, saliva was collected from each mosquito and inoculated onto C6/36 mosquito cells to check for the presence of CHIKV infectious particles. Legs and body (thorax and abdomen of each mosquito were also collected at the different dpi and submitted separately to viral RNA extraction and CHIKV real-time RT-PCR.CHIKV infection rate, dissemination and transmission efficiencies ranged from 7-90%, 18-78% and 5-53% respectively for Ae. aegypti and from 39-41%, 3-17% and 0-14% respectively for Ae. polynesiensis, depending on the dpi. Infectious saliva was found as early as 2 dpi for Ae. aegypti and from 6 dpi for Ae. polynesiensis. Our laboratory results confirm that the French Polynesian population of Ae. aegypti is highly competent for CHIKV and they provide clear evidence for Ae. polynesiensis to act as an efficient CHIKV vector.As supported by our findings, the presence of two CHIKV competent vectors in French Polynesia certainly contributed to enabling this virus to quickly disseminate from the urban/peri-urban areas colonized by Ae. aegypti to the most remote atolls where Ae. polynesiensis is predominating. Ae. polynesiensis was probably involved in the recent chikungunya outbreaks in Samoa and the Cook Islands. Moreover, this vector may contribute to the risk for CHIKV to emerge in other Polynesian islands like Fiji, and more particularly Wallis where there is no Ae. aegypti.

  15. PENGARUH FREKUENSI PENGHISAPAN DARAH TERHADAP PERKEMBANGAN, REPRODUKSI,VERTILITAS DAN RASIO SEX Aedes aegypti

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    Riyani Setiyaningsih

    2014-06-01

    Full Text Available Aedes aegypti is a vector of Dengue hemorrhagic fever in Indonesia. Aedesaegypti has a high reproduction ability. Each individual can produce 50-100 eggs. Which80% of them are fertile. The mosquito is multiple biting (which means each individualsucks blood several time. Based on that background, this research was aimed torecognize the frequency of blood sucking to development, reproduction, fertility, and sexratio of Ae. aegypti. Thirty Ae. aegypti mosquito were put into the plastic cupsindividually, then fed with mammals. The treatments were the first, second, third, fourth,and fifth blood sucking. The eggs produced in each blood sucking were hatched andmaintain to become mosquitoes. The parameter measured from each blood sucking istotal egg production, egg fertility, larvae mortality, pupae mortality, and sex ratio. Theresult of the research shows that the frequency of blood sucking affects the production ofegg fertility, but does not affect the total egg production, larvae mortality, pupaemortality, and sex ratio significantly.Key words: sex ratio, egg fertility, reproduction Aedes aegypti adalah vektor Demam berdarah dengue di Indonesia. Ae aegyptimempunyai kemampuan berkembang biak dengan cepat. Setiap individu mempunyaikemampuan menghasilkan telur 50 sampai 100 ekor skali bertclur. Ae. aegypti bersifat multibiting, masing-masing individu mempunyai kemampuan menghisap darah beberapa kali dalamkurun waktu tertentu. Berdasarkan latar belakang tersebut tujuan penelitian ini adalahmendapatkan pengaruh frekuensi penghisapan darah terhadap perkembangan reproduksi,fcrtilitas, dan rasio sex dari Ae. aegypti. Ae aegypti dimasukkan ke dalam cup plastik secaraindividual, kemudian diberikan darah mamalia selama kurang lebih 3 menit. Pemberian darahdilakukan secara bertahap yaitu pemberian darah pertama, kedua, ketiga, ke empat, dan ke limaTelur-telur yang dihasilkan pada masing-masing penghisapan darah di tetaskan dan dipeliharasampai menjadi nyamuk

  16. Allergens involved in the cross-reactivity of Aedes aegypti with other arthropods.

    Science.gov (United States)

    Cantillo, Jose Fernando; Puerta, Leonardo; Lafosse-Marin, Sylvie; Subiza, Jose Luis; Caraballo, Luis; Fernandez-Caldas, Enrique

    2017-06-01

    Cross-reactivity between Aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described. To evaluate the cross-reactivity between A aegypti and other arthropods. Thirty-four serum samples from patients with asthma and/or allergic rhinitis were selected, and specific IgE to A aegypti, Dermatophagoides pteronyssinus, Dermatophagoides farinae, Blomia tropicalis, Periplaneta americana. and Litopenaeus vannamei was measured by enzyme-linked immunosorbent assay. Cross-reactivity was investigated using pooled serum samples from allergic patients, allergenic extracts, and the recombinant tropomyosins (Aed a 10.0201, Der p 10, Blo t 10, Lit v 1, and Per a 7). Four IgE reactive bands were further characterized by matrix-assisted laser desorption/ionization tandem time of flight. Frequency of positive IgE reactivity was 82.35% to at least one mite species, 64.7% to A aegypti, 29.4% to P americana, and 23.5% to L vannamei. The highest IgE cross-reactivity was seen between A aegypti and D pteronyssinus (96.6%) followed by L vannamei (95.4%), B tropicalis (84.4%), and P americana (75.4%). Recombinant tropomyosins from mites, cockroach, or shrimp inhibited the IgE reactivity to the mosquito at a lower extent than the extracts from these arthropods. Several bands of A aegypti cross-reacted with arthropod extracts, and 4 of them were identified as odorant binding protein, mitochondrial cytochrome C, peptidyl-prolyl cis-trans isomerase, and protein with hypothetical magnesium ion binding function. We identified 4 novel cross-reactive allergens in A aegypti allergenic extract. These molecules could influence the manifestation of allergy to environmental allergens in the tropics. Copyright © 2017 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  17. Asymmetrical Competition between Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae Coexisting in Breeding Sites

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    Juan C. Santana-Martínez

    2017-10-01

    Full Text Available Aedes aegypti and Culex quinquefasciatus are mosquito vectors for several tropical diseases that represent a current public health problem. The ecological requirements for each species are different, however, both species show high biological adaptability, which promotes their coexistence in the same breeding sites. The purpose of this study was to assess the effect of larval association between Ae. aegypti and Cx. quinquefasciatus under different laboratory conditions of food supply and temperature, and under field simulated conditions like peridomestic containers. Our findings showed that under field simulated conditions there was no asymmetrical competition in mixed cultures with the different Cx. quinquefasciatus/Ae. aegypti ratios tested. However, under laboratory conditions in which different doses of food supply were evaluated, it was observed that competition between the two species takes place. Larval coexistence under food scarcity conditions (0.95 mg/larva showed that Ae. aegypti had a greater adult emergence than Cx. quinquefasciatus and was capable of depriving Cx. quinquefasciatus of the food needed to complete metamorphosis. In an intermediate dose of food (1.9 mg/larva, the dry weight of Cx. quinquefasciatus adults decreased, and their larval development time increased when Cx. quinquefasciatus/Ae. aegypti ratio was low. Also, a temperature effect was assessed demonstrating that Cx. quinquefasciatus was more vulnerable to changes in temperature. We suggest that Ae. aegypti is more successful in exploiting microhabitats when food is scarce, due to its scrape active feeding habitats and fast larval development times. Therefore, in conditions of food paucity both species will compete, and Ae. aegypti larvae will prevail.

  18. The perception and use of insects by the residents of Ribeirão da Ilha, Florianópolis, SC, Brazil

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    Mônica Antunes Ulysséa

    2010-09-01

    Full Text Available This work presents the perception of the residents of Ribeirão da Ilha, Florianópolis, Santa Catarina Island, about the insects and the distinct uses made of them. The study was carried out between August and October of 2007 through semi-structured interviews with 50 residents of the community. The ethnocategory ‘Insect’ is composed by 16 animals such as bees, cockroaches, beetles, spiders, snakes and others. This category is mostly seen negatively by the local population, or causes a sensation of fear and disgust. The knowledge about the use of insects is wide and it is related with ludic, decorative, alimentary and medicinal usages, as well as with forecasting the weather and earning money. However, nowadays only three uses are practiced by the community: consumption of honey in food, employment of bee stings to cure rheumatism, and butterflies for decoration.

  19. 14C dating and isotopic composition of mangrove soil in the Parque Estadual da Ilha do Cardoso, Southern Sao Paulo State

    International Nuclear Information System (INIS)

    Vidotto, Elaine; Pessenda, Luiz Carlos Ruiz; Gouveia, Susy Eli M.; Bendassolli, Jose Albertino; Ricardi-Branco, Fresia; Aravena, Ramon

    2007-01-01

    This work is part of a study involving paleoenvironmental records based on pollen analysis, isotopic composition ( 12 C, 13 C and 14 N, 15 N) and 14 C dating of mangrove soil. Samplings have been made in the Jacariu mangrove located at Parque Estadual da Ilha do Cardoso, Cananeia, southern Sao Paulo state. This study must improve significantly the studies of vegetation and climate changes that occurred during the late Holocene (approximately the last 2000 years) in the southeastern region of Brazil. The δ 13 C results of mangrove soil presented values from -24 to -26 per mille The values of TOC and C/N associated with δ 13 C values indicated the predominance of organic matter from C 3 land plant and phytoplankton (algal) in the whole profile. The 14 C dating indicated Modern age at the shallow horizons to 1810 years B.P. for the deeper horizon. (author)

  20. QUALIDADE DE VIDA E CONDIÇÕES PARA SE VIVER DOS MARICULTORES: UM ESTUDO EXPLORATÓRIO NO RIBEIRÃO DA ILHA, FLORIANÓPOLIS – SC

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    Letícia Saidy

    2014-08-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Atualmente a maricultura é um setor de destaque em Santa Catarina. Com a introdução da espécie exótica de ostra, Crassostrea gigas, em 1994, o estado despontou como líder nacional na produção desses moluscos. Por ser a maricultura um dos setores produtivos mais ativos do estado de Santa Catarina, desenvolveu-se uma pesquisa que utilizou dois indicadores como instrumentos conceituais e metodológicos para a diferenciação e avaliação da qualidade de vida e da qualidade de condições para viver: Indicador da Qualidade de Vida (IQV e o Indicador das Condições para se Viver (IQCV. O objetivo principal do estudo foi apontar possíveis diferenças de olhares entre produtores familiares de ostras e os agentes de desenvolvimento da EPAGRI. Os indicadores IQCV e IQV foram empregados e adequados a localidade do Ribeirão da Ilha, Florianópolis – SC. Comparando-se o IQV (0,66 com IQCV (0,50, compreende-se que o grau de satisfação com a qualidade das condições de vida manifestada pelos maricultores é inferior que a percebida pela equipe de apoio da EPAGRI. O resultado demonstra que algumas melhorias nas condições de vida propostas pelos técnicos da EPAGRI nem sempre podem ser traduzidas como uma melhoria da qualidade

  1. Developing Sterile Insect Technique (SIT) as a tool Mosquito Control Districts can use for integrated Aedes aegypti control

    Science.gov (United States)

    New tools are clearly needed for integrated mosquito management of Ae. aegypti. We describe the sterile insect technique (SIT) that we are developing as a method to control Ae. aegypti by partnering with two prominent Florida mosquito control districts (MCD) and the FAO/IAEA Insect Pest Control Sub...

  2. Hallazgo de mesocyclops aspericornis (Daday) (Copepoda: Cyclopidae) depredador de larvas de aedes aegypti en Anapoima Colombia (1)

    OpenAIRE

    Marco Fidel Suárez; Dwight Ayala; Michael J. Nelson; Janet W. Reid

    1984-01-01

    En Anapoima, Colombia, se encontró que el copépodo Mesocyclops aspericornis era depredador de larvas del mosquito Aedes aegypti. Este encuentro representa el primer hallazgo de este copépodo en recipientes artificiales en la región neotropical, y el primer hallazgo como depredador de larvas de Aedes aegypti.

  3. A new record of the Restinga Tyrannulet Phylloscartes kronei (Aves, Rynchocyclidae at Ilha do Cardoso State Park, southeastern Brazil (Scientific Note. Novo registro de maria-da-restinga, Phylloscartes kronei (Aves, Rynchocyclidae no Parque Estadual Ilha do Cardoso, Sudeste do Brasil (Nota Científica

    Directory of Open Access Journals (Sweden)

    Augusto João PIRATELLI

    2014-06-01

    Full Text Available During an expedition to Ilha do Cardoso State Park, in Cananéia, São Paulo, Brazil, two individuals of the Restinga Tyrannulet (Phylloscartes kronei, Aves: Rynchocyclidae were captured with a 36 mm mesh mist net in a restinga area (25o 12.243’S and 47o 59.502’W at Núcleo Marujá. This random record highlights the relevance of this protected area for this globally threatened species (vulnerable, where it is apparently abundant, due to the existence of suitable sites for its survival and reproduction. Durante uma expedição ao Parque Estadual Ilha do Cardoso, em Cananéia, SP, dois indivíduos de maria-da-restinga (Phylloscartes kronei, Aves: Rynchocyclidae foram capturados com uma rede de neblina de malha 36 mm em uma área de restinga (25o 12.243’S e 47o 59.502’O, no Núcleo Marujá. Esse registro fortuito ressalta a relevância desta Unidade de Conservação para esta espécie globalmente ameaçada (vulnerável, onde ela aparentemente é abundante, devido à existência de habitats adequados à sua sobrevivência e reprodução.

  4. QTL mapping of genome regions controlling temephos resistance in larvae of the mosquito Aedes aegypti.

    Science.gov (United States)

    Reyes-Solis, Guadalupe Del Carmen; Saavedra-Rodriguez, Karla; Suarez, Adriana Flores; Black, William C

    2014-10-01

    The mosquito Aedes aegypti is the principal vector of dengue and yellow fever flaviviruses. Temephos is an organophosphate insecticide used globally to suppress Ae. aegypti larval populations but resistance has evolved in many locations. Quantitative Trait Loci (QTL) controlling temephos survival in Ae. aegypti larvae were mapped in a pair of F3 advanced intercross lines arising from temephos resistant parents from Solidaridad, México and temephos susceptible parents from Iquitos, Peru. Two sets of 200 F3 larvae were exposed to a discriminating dose of temephos and then dead larvae were collected and preserved for DNA isolation every two hours up to 16 hours. Larvae surviving longer than 16 hours were considered resistant. For QTL mapping, single nucleotide polymorphisms (SNPs) were identified at 23 single copy genes and 26 microsatellite loci of known physical positions in the Ae. aegypti genome. In both reciprocal crosses, Multiple Interval Mapping identified eleven QTL associated with time until death. In the Solidaridad×Iquitos (SLD×Iq) cross twelve were associated with survival but in the reciprocal IqxSLD cross, only six QTL were survival associated. Polymorphisms at acetylcholine esterase (AchE) loci 1 and 2 were not associated with either resistance phenotype suggesting that target site insensitivity is not an organophosphate resistance mechanism in this region of México. Temephos resistance is under the control of many metabolic genes of small effect and dispersed throughout the Ae. aegypti genome.

  5. Heat Sensitivity of wMel Wolbachia during Aedes aegypti Development.

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    Jill N Ulrich

    2016-07-01

    Full Text Available The wMel strain of Wolbachia bacteria is known to prevent dengue and Zika virus transmission in the mosquito vector Aedes aegypti. Accordingly, the release of wMel-infected A. aegypti in endemic regions has been recommended by the World Health Organization as a potential strategy for controlling dengue and Zika outbreaks. However, the utility of this approach could be limited if high temperatures in the aquatic habitats where A. aegypti develop are detrimental to Wolbachia. We exposed wMel-infected A. aegypti eggs and larvae to fluctuating daily temperatures of 30-40°C for three, five, or seven days during their development. We found that Wolbachia levels in females emerging from heat treatments were significantly lower than in the controls that had developed at 20-30°C. Notably, seven days of high temperatures starting at the egg stage reduced Wolbachia levels in emerging females to less than 0.1% of the wMel control levels. However, after adult females returned to 20-30°C for 4-7 days, they experienced differing degrees of Wolbachia recovery. Our findings suggest that the spread of Wolbachia in wild A. aegypti populations and any consequent protection from dengue and Zika viruses might be limited in ecosystems that experience periods of extreme heat, but Wolbachia levels recover partially after temperatures return to normal.

  6. Genome Investigations of Vector Competence in Aedes aegypti to Inform Novel Arbovirus Disease Control Approaches

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    David W. Severson

    2016-10-01

    Full Text Available Dengue (DENV, yellow fever, chikungunya, and Zika virus transmission to humans by a mosquito host is confounded by both intrinsic and extrinsic variables. Besides virulence factors of the individual arboviruses, likelihood of virus transmission is subject to variability in the genome of the primary mosquito vector, Aedes aegypti. The “vectorial capacity” of A. aegypti varies depending upon its density, biting rate, and survival rate, as well as its intrinsic ability to acquire, host and transmit a given arbovirus. This intrinsic ability is known as “vector competence”. Based on whole transcriptome analysis, several genes and pathways have been predicated to have an association with a susceptible or refractory response in A. aegypti to DENV infection. However, the functional genomics of vector competence of A. aegypti is not well understood, primarily due to lack of integrative approaches in genomic or transcriptomic studies. In this review, we focus on the present status of genomics studies of DENV vector competence in A. aegypti as limited information is available relative to the other arboviruses. We propose future areas of research needed to facilitate the integration of vector and virus genomics and environmental factors to work towards better understanding of vector competence and vectorial capacity in natural conditions.

  7. Differentiation of Aedes aegypti and Aedes notoscriptus (Diptera: Culicidae) eggs using scanning electron microscopy.

    Science.gov (United States)

    Faull, Katherine J; Williams, Craig R

    2016-05-01

    Aedes notoscriptus and Aedes aegypti are both peri-domestic, invasive container-breeding mosquitoes. While the two potential arboviral vectors are bionomically similar, their sympatric distribution in Australia is limited. In this study, analyses of Ae. aegypti and Ae. notoscriptus eggs were enabled using scanning electron microscopy. Significant variations in egg length to width ratio and outer chorionic cell field morphology between Ae. aegypti and Ae. notoscriptus enabled distinction of the two species. Intraspecific variations in cell field morphology also enabled differentiation of the separate populations of both species, highlighting regional and global variation. Our study provides a comprehensive comparative analysis of inter- and intraspecific egg morphological and morphometric variation between two invasive container-breeding mosquitoes. The results indicate a high degree of intraspecific variation in Ae. notoscriptus egg morphology when compared to the eggs of Ae. aegypti. Comparative morphological analyses of Ae. aegypti and Ae. notoscriptus egg attributes using SEM allows differentiation of the species and may be helpful in understanding egg biology in relation to biotope of origin. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Insecticide susceptibility of the dengue vector Aedes aegypti (Diptera: culicidae in Makkah City, Saudi Arabia

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    Al Thbiani Aziz

    2011-06-01

    Full Text Available Objective: To examine the insecticide susceptibility of Aedes aegypti (Ae. aegypti from various sites in Makkah City, Saudi Arabia. Methods: This was examined based on WHO standard procedures. Results: The larvae of Ae. aegypti were susceptible to all larvicides examined, but this susceptibility was more pronounced in wild populations, which tended to show tolerance to icon. Icon was the most effective larvicide with LC 50 values of 0.007 ppm and 0.012 ppm for the laboratory and field strains, respectively. Ae. aegypti adults exposed to lambda-cyhalothrin showed a low mortality rate in comparison with those exposed to deltamethrin and cyfluthrin. Conclusions: The results of the present study indicate differential susceptibility between field and laboratory larval populations. Wild larvae are less susceptible to insecticide treatments than their laboratory-bred counterparts. Taken together, these results suggest that tolerance and the tendency toward resistance to commonly used insecticides are present in Ae. aegypti populations throughout Makkah City, Saudi Arabia.

  9. Local evolution of pyrethroid resistance offsets gene flow among Aedes aegypti collections in Yucatan State, Mexico.

    Science.gov (United States)

    Saavedra-Rodriguez, Karla; Beaty, Meaghan; Lozano-Fuentes, Saul; Denham, Steven; Garcia-Rejon, Julian; Reyes-Solis, Guadalupe; Machain-Williams, Carlos; Loroño-Pino, Maria Alba; Flores-Suarez, Adriana; Ponce-Garcia, Gustavo; Beaty, Barry; Eisen, Lars; Black, William C

    2015-01-01

    The mosquito Aedes aegypti is the major vector of the four serotypes of dengue virus (DENV1-4). Previous studies have shown that Ae. aegypti in Mexico have a high effective migration rate and that gene flow occurs among populations that are up to 150 km apart. Since 2000, pyrethroids have been widely used for suppression of Ae. aegypti in cities in Mexico. In Yucatan State in particular, pyrethroids have been applied in and around dengue case households creating an opportunity for local selection and evolution of resistance. Herein, we test for evidence of local adaptation by comparing patterns of variation among 27 Ae. aegypti collections at 13 single nucleotide polymorphisms (SNPs): two in the voltage-gated sodium channel gene para known to confer knockdown resistance, three in detoxification genes previously associated with pyrethroid resistance, and eight in putatively neutral loci. The SNPs in para varied greatly in frequency among collections, whereas SNPs at the remaining 11 loci showed little variation supporting previous evidence for extensive local gene flow. Among Ae. aegypti in Yucatan State, Mexico, local adaptation to pyrethroids appears to offset the homogenizing effects of gene flow. © The American Society of Tropical Medicine and Hygiene.

  10. Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector.

    Science.gov (United States)

    Seixas, Gonçalo; Salgueiro, Patrícia; Silva, Ana Clara; Campos, Melina; Spenassatto, Carine; Reyes-Lugo, Matías; Novo, Maria Teresa; Ribolla, Paulo Eduardo Martins; Silva Pinto, João Pedro Soares da; Sousa, Carla Alexandra

    2013-01-01

    The increasing population of Aedes aegypti mosquitoes on Madeira Island (Portugal) resulted in the first autochthonous dengue outbreak, which occurred in October 2012. Our study establishes the first genetic evaluation based on the mitochondrial DNA (mtDNA) genes [cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4)] and knockdown resistance (kdr) mutations exploring the colonisation history and the genetic diversity of this insular vector population. We included mosquito populations from Brazil and Venezuela in the analysis as putative geographic sources. The Ae. aegypti population from Madeira showed extremely low mtDNA genetic variability, with a single haplotype for COI and ND4. We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C). These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance. Finally, we also report the presence of the F1534C kdr mutation in the Brazil and Venezuela populations. To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes. Given the present risk of Ae. aegypti re-invading continental Europe from Madeira and the recent dengue outbreaks on the island, this information is important to plan surveillance and control measures.

  11. Potensi Tanaman Di Indonesia Sebagai Larvasida Alami Untuk Aedes aegypti

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    Yoke Astriani

    2017-02-01

    Full Text Available AbstractDengue Hemorrhagic Fever (DHF still become one of the health problems in Indonesia. This disease has emerged since 1968 and has become one of the deadliest disease in various regions in Indonesia. Indonesia has not implemented an effective dengue vaccine program to provide protection against all four serotypes of the dengue virus. Vector control using chemical insecticides has made the vectors resistant to the insectisides. One option to avoid those bad effect is natural larvicide. The lack of literature that can be used as a foundation for further studies on the natural larvicidal is the reason why a review of several articles and research results is needed. We use the method of literature review. Our literature reveals that 68% of the 25 plants species are categorized as highly effective with LC50 <750 ppm. Jasmine, Zodia and Tobacco have the highest effectivity compared to the other plants use in this literature review. The LC50 of these plants are 0.999 ppm, 1.94 ppm, and 1.94 ppmrespectively. All twenty five plants that we present suitable to be cultivated in the region of Indonesia with tropical climate so that people can easily cultivate and use it as a natural larvicides.Keyword: Natural larvicides, Aedes aegypti, LC50, and essential oilAbstrakPenyakit Demam Berdarah Dengue (DBD sampai saat ini masih menjadi salah satu permasalahan dunia kesehatan di Indonesia. Penyakit yang muncul sejak tahun 1968 ini telah banyak menelan korban di berbagai daerah dan hingga saat ini, Indonesia belum menerapkan program vaksin yang efektif untuk memberikan perlindungan terhadap empat serotipe dari virus dengue. Pengendalian vektor menggunakan bahan insektisida kimiawi banyak memberikan efek resisten terhadap insektisida tersebut. Salah satu pilihan untuk menghindari hal tersebut dibutuhkan adanya larvasida alami. Oleh karena masih tersebarnya informasi yang dapat dijadikan dasar untuk studi lanjut mengenai larvasida alami, maka tulisan ini

  12. CRESCIMENTO URBANO DESORDENADO NO MUNICÍPIO DE PONTA DE PEDRAS NA ILHA DO MARAJÓ: um estudo de caso do bairro do Carnapijó

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    João Gabriel Pinheiro Huffner

    2017-08-01

    El crecimiento urbano desordenado representa una realidad común a la mayoría de las comarcas brasileñas, hecho que escapa al control de las diversas instituciones y órganos de planeamiento del uso y ocupación del suelo urbano, generando nuevos desafíos a la gobernanza urbana local, Incluyendo la mitigación de los diversos problemas sociales, ambientales y económicos que emergen de las relaciones desencadenadas por la ausencia de acciones concretas en el contexto de las políticas sociales e intervenciones estructuráis. Así, se revela en cuanto objeto de estudio de esta pesquisa, la comarca de Ponta de Pedras en el Estado de Pará, con recorte especial al barrio de Carnapijó, teniendo como objetivo central discutir su dinámica del crecimiento urbano desordenado caracterizada por la ocupación de áreas impropias, viviendas precarias y la falta de acceso de la población que habita el barrio a servicios básicos, reflexionando sobre posibles causas y consecuencias y sus inflexiones con las debilidades político-administrativas locales. En este sentido en cuanto procedimientos metodológicos se ha adoptado la pesquisa bibliográfica para subsidiar la discusión pertinente al tema y, Por lo tanto, la aplicación de entrevistas a los vecinos del barrio, con el objetivo de identificar los principales factores que componen el escenario de degradación humana, social y ambiental proveniente del crecimiento urbano desordenado del barrio de Carnapijó en Ponta de Pedras. Los resultados obtenidos apuntan a un escenario de precariedad estructural, basado en la ausencia de servicios básicos y en la inercia del poder público frente al proceso de ocupación desordenada de Carnapijó. Palabras-clave: Ocupación Desordenada; Ordenación Territorial; Periferización.

  13. Assimetrias Regionais no Turismo e Novas Estratégias na Oferta Turística: O caso da Ilha da Madeira

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    Filipa Fernandes

    2015-01-01

    Full Text Available This study aims to analyze tourism in Madeira Island, emphasizing the differences between the north coast and south coast. With a secular tradition in tourism, this Portuguese Autonomous Region reveals tourism development strategies of several destinations (internal and emerging products. Firstly, some trends in regional tourism market will be characterized, and secondly, we will describe new strategies concerning tourism related to nature tourism.

  14. Aspectos de infraestrutura e serviços como indicadores da turistificação na Vila de Encantadas, Ilha do Mel (Brasil

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    Daniel Hauer Queiroz Telles

    2011-01-01

    Full Text Available The isle of Mel represents the reality of some insular touristic destinations in the Brazilian coasts, with very special peculiarities raging from the image of an alternative destination to the fragility of social and environmental issues. It has different characteristics, (lack of automobiles and the predominance of small inns, that began since the touristic exploration almost four decades ago. The purpose of the present article is to analyze the conditions of the infrastructure and the services as a result of the process of touristification in Villa Encantada in the isle of Mel (Brazil, as a way to understand the effects on the conformation of such destination. This project was carried out through bibliographical and documental investigation. In addition, a field investigation was conducted based on interviews with the social actors which were members of the local government. From the main results, some specific aspects are of particular interest in relation with the conditions of the infrastructure and the services for both the tourism and the society. Such disparity testifies that the collapse of the touristic destination reached its maximum point between 2006 as a consequence of an irregular touristification that happened in the middle of 1990 and at the end of century.

  15. Breve notícia da recente erupção na ilha do Fogo e suas consequências

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    Fernando Costa

    1995-06-01

    Full Text Available BRIEF NOTES ABOUT THE RECENT VOLCANIC ERUPTION AT FOGO ISLAND - This article provides a concise description of the volcanic eruption that occurred in 1995 in the island of Fogo (Cape Verde. It focuses on the evolution of the eruption, its characteristics, and the alteration it produced on the topography and morphology of the landscape of Chã das Caldeiras. The consequences of this phenomenon on the human and agricultural occupation are also emphasized.

  16. Tensões entre pesca, turismo e exploração de gás reconfigurando ruralidade na ilha de Boipeba – BA

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    Cristina Maria Macêdo de Alencar

    2011-01-01

    Full Text Available As we realize that development is a historical process in constant movement that generates advances and setbacks to overcome social inequalities - currently known as asymmetries - this article examines the social daily dynamics on the island of Boipeba, city of Cairu, Bahia, and member of APA Tinharé - Boipeba, which is impacted by environmental issues and developing tourism in Costa do Dendê. In that process the appearance of gas exploration in January 2007 is periodized, when the activities of gas production began in the Manati Field BCAM-40, located in the Camamu-Almada Basin, which comes with specific environmental regulations. It is questioned whether the compliance with environmental regulations has interfered in the life of the population of the island, giving importance to fishing as an economic activity that structures the site in its rural way of life in relation to tourism and gas production as exogenous induction. The research was based on documental and field data (2009 and 2010, and its results show that tourist activities and gas exploration constitute a (dis organizational phenomenon of the rural fishing that seems to have been strained with the mismanagement of environmental agents since the studies of environmental impact, through the environmental impact report and coming up with the increase of asymmetry compensation for environmental intervention.

  17. Observações sobre a história natural do lagarto Mabuya macrorhyncha Hoge (Scincidae) na Ilha da Queimada Grande, São Paulo, Brasil

    OpenAIRE

    Vrcibradic, Davor; Rocha, Carlos F. D.

    2005-01-01

    Data are presented on the on diet, thermal biology, microhabitat use, reproduction and helminth infection parameters of a population of Mabuya macrorhyncha hoge, 1946 inhabiting the small island of Queimada Grande, on the southern coast of São Paulo State. The lizards were collected on November 1997 (spring) and on July 1998 (winter). Most lizards were perched above ground, mainly on herbaceous vegetation. Mean lizard body temperatures were significantly higher in spring (32.6 ± 1.9ºC) than i...

  18. Avaliação da resiliência em idosos na ilha de São Miguel numa perspectiva multidimensional

    OpenAIRE

    Silva, Osvaldo; Sousa, Áurea

    2013-01-01

    V Congresso - Envelhecimento(s): A arte de viver, 3 e 4 outubro de 2013, Ponta Delgada, Açores, Portugal (Comunicação). Programa: http://www.alv.uac.pt/uploads/uac_artigo_plugin/ficheiro/desdobr%C3%A1vel%20do%20congresso.pdf A resiliência é a capacidade de um indivíduo superar as adversidades da vida e transformá-las de forma positiva. A resiliência varia ao longo do tempo, de acordo com as mudanças ocorridas em cada ser humano, e é um processo dinâmico, que é resultante do equilíbrio e...

  19. Trilho da Ciência: um percurso de Educação Científica na ilha do Príncipe

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    Joana Latas

    2015-07-01

    Full Text Available Framed in an integrated vision of formal and non-formal educational contexts, the pathway of scientific education in Príncipe Island - Science Trail, was conceptualized, implemented and assessed. Science Trail provides contact with nature and assumes the exploration of scientific contents, integrated in a historical and cultural Principean context. Moreover, cultural contexts have multidisciplinary potentialities which, when recognized and legitimated, enhance a holistic view of science (Gerdes, 2007.In particular, some of those cultural and historical contexts proved to be facilitators in the process of building bridges with mathematical concepts, enhancing connections between culture and mathematics. The drawing up of this trail was the responsibility of a multidisciplinary team of Principe’s High school teachers and its implementation targeted mainly high school students. The evaluation of the Science Trail, namely its impact on participants, based on data gathered through questionnaires and document compilation, points to the continuity of this initiative and to the recognition of its educational potentialities.

  20. A apanha de algas na ilha da Ínsua (Caminha nos séculos XVII - XIX. Singularidades e conflitos.

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    João Paulo Cabral

    2005-12-01

    Full Text Available SEAWEED GATHERING ON ÍNSUA ISLAND (CAMINHA IN THE 17TH TO 19TH CENTURIES. SINGULARITIES AND CONFLICTS. Seaweed gathering along the northwestern coast of mainland Portugal constituted, from the Middle Ages to the 19th century, an extremely important activity. This is well documented for the region located between Viana do Castelo and Oporto, but much less is known for other regions along the northwestern coast. In the Braga District Archive there is a valuable set of unpublished manuscripts about the Franciscan monastery of Ínsua (an island located in the mouth of the River Minho and about seaweed gathering on the island in the past. By analysing these documents and studying the present macroalgal flora of the island, it was possible to conclude that seaweed gatheringon Ínsua in the 17th to 19th centuries was not free to all, as it was on the mainland coast, but rather relied on permission granted by the monastery’s guardian. Gathering was not allowed on Sundays and holidays. Women were not allowed to gather seaweed and men were required to leave the island before nightfall. Seaweed was exchanged for collectors’ donations to the friars. Seaweed was collected from the shore at low tide, and Fucus and Ascophyllum nodosum plants were likely cut from the rocks. Seaweed gathering was a constant source of intense conflict and struggle between the Franciscans, their neighbours and the fortress garrison. The main factors determining the particularities related to seaweed gathering at Ínsua include the long presence of the Franciscan community on the island, the right to all the island’s natural resources ceded by the Crown to the Franciscans and elevated economical importance of seaweed for the agrarian societies of the northwestern coast. Other factors involve the following: i the great abundance of seaweed on the island may have fostered greed; ii most of the community abandoned the island for a monastery in Caminha in 1619 and only one friar lived on the island in 1793, making for a possible sense of emptiness and absence – the neighbours, therefore, considered it unjust that the island’s wealth stay in the hands of the Franciscans; iii economic difficulties arose, since the endowment by the Crown remained unchanged for more than a century; and iv collectors were clearly fortunate that seaweed gathering on the mainland coast was free of charge.

  1. Sindbis virus infection alters blood feeding responses and DEET repellency in Aedes aegypti (Diptera: Culicidae).

    Science.gov (United States)

    Qualls, Whitney A; Day, Jonathan F; Xue, Rui-De; Bowers, Doria F

    2012-03-01

    Aedes aegypti (L.) (Diptera: Culicidae) female mosquitoes infected systemically with Sindbis virus (SINV) took longer than uninfected mosquitoes to locate and fully engorge on blood. On days 7 and 14 postexposure, blood feeding took 1.3 and 1.5 times longer in mosquitoes with a disseminated SINV infection, respectively. SINV dissemination did not affect the average weight of unfed Ae. aegypti, but did result in a 10 and 12% increase in blood imbibed compared with mosquitoes without a positive SINV dissemination and non-SINV-exposed mosquitoes, respectively. Ae. aegypti mosquitoes with a disseminated SINV infection fed an average of 4 h sooner than uninfected mosquitoes when offered a bloodmeal contained inside a DEET (N,N-diethyl-3-methylbenzamide) saturated (30%) bovine sausage casing. Together, these results indicate that behavioral changes in mosquito host-seeking, blood feeding and sensitivity to DEET occurred in mosquitoes after SINV infection and dissemination.

  2. Oral susceptibility of Aedes aegypti (Diptera: Culicidae) from Senegal for dengue serotypes 1 and 3 viruses.

    Science.gov (United States)

    Gaye, Alioune; Faye, Oumar; Diagne, Cheikh T; Faye, Ousmane; Diallo, Diawo; Weaver, Scott C; Sall, Amadou A; Diallo, Mawlouth

    2014-11-01

    To investigate the potential for domestic and wild populations of Aedes aegypti from Dakar and Kedougou to develop a disseminated infection after exposure to DENV-3 and DENV-1. We have exposed sylvatic and urban population of Ae. aegypti from Senegal to bloomeals containing dengue serotype 1 and 3. At different incubation period, individual mosquito legs/wings and bodies were tested for virus presence using real time RT-PCR to estimate the infection and dissemination rates. The data indicated low susceptibility to DENV-3 (infection: 2.4-15.2%, and dissemination rates: 0-8.3%) and higher susceptibility to DENV-1 (infection and dissemination rates up to 50%). Aedes aegypti from Senegal seem able to develop a disseminated infection of DENV-1 and DENV-3. Further studies are needed to test their ability to transmit the two serotypes. © 2014 John Wiley & Sons Ltd.

  3. Competence of Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus Mosquitoes as Zika Virus Vectors, China

    Science.gov (United States)

    Liu, Zhuanzhuan; Zhou, Tengfei; Lai, Zetian; Zhang, Zhenhong; Jia, Zhirong; Zhou, Guofa; Williams, Tricia; Xu, Jiabao; Gu, Jinbao; Zhou, Xiaohong; Lin, Lifeng; Yan, Guiyun

    2017-01-01

    In China, the prevention and control of Zika virus disease has been a public health threat since the first imported case was reported in February 2016. To determine the vector competence of potential vector mosquito species, we experimentally infected Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus mosquitoes and determined infection rates, dissemination rates, and transmission rates. We found the highest vector competence for the imported Zika virus in Ae. aegypti mosquitoes, some susceptibility of Ae. albopictus mosquitoes, but no transmission ability for Cx. quinquefasciatus mosquitoes. Considering that, in China, Ae. albopictus mosquitoes are widely distributed but Ae. aegypti mosquito distribution is limited, Ae. albopictus mosquitoes are a potential primary vector for Zika virus and should be targeted in vector control strategies. PMID:28430562

  4. Competence of Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus Mosquitoes as Zika Virus Vectors, China.

    Science.gov (United States)

    Liu, Zhuanzhuan; Zhou, Tengfei; Lai, Zetian; Zhang, Zhenhong; Jia, Zhirong; Zhou, Guofa; Williams, Tricia; Xu, Jiabao; Gu, Jinbao; Zhou, Xiaohong; Lin, Lifeng; Yan, Guiyun; Chen, Xiao-Guang

    2017-07-01

    In China, the prevention and control of Zika virus disease has been a public health threat since the first imported case was reported in February 2016. To determine the vector competence of potential vector mosquito species, we experimentally infected Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus mosquitoes and determined infection rates, dissemination rates, and transmission rates. We found the highest vector competence for the imported Zika virus in Ae. aegypti mosquitoes, some susceptibility of Ae. albopictus mosquitoes, but no transmission ability for Cx. quinquefasciatus mosquitoes. Considering that, in China, Ae. albopictus mosquitoes are widely distributed but Ae. aegypti mosquito distribution is limited, Ae. albopictus mosquitoes are a potential primary vector for Zika virus and should be targeted in vector control strategies.

  5. Larvicidal Activity of essential oils from Brazilian plants against Aedes aegypti L.

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    Eveline Solon Barreira Cavalcanti

    2004-08-01

    Full Text Available Aedes aegypti L. is the major vector of dengue fever, an endemic disease in Brazil. In an effort to find effective and affordable ways to control this mosquito, the larvicidal activities of essential oils from nine plants widely found in the Northeast of Brazil were analyzed by measurement of their LC50. The essential oils were extracted by steam distillation and their chemical composition determined by GL-chromatography coupled to mass spectroscopy. The essential oils from Cymbopogon citratus and Lippia sidoides, reported in the literature to have larvicidal properties against A. aegypti, were used for activity comparison. The results show that Ocimum americanum and Ocimum gratissimum have LC50 of 67 ppm and 60 ppm respectively, compared to 63 ppm for L. sidoides and 69 ppm for C. citratus. These results suggest a potential utilization of the essential oil of these two Ocimum species for the control of A. aegypti.

  6. Determination of the efficiency of diets for larval development in mass rearing Aedes aegypti (Diptera: Culicidae).

    Science.gov (United States)

    Gunathilaka, P A D H N; Uduwawala, U M H U; Udayanga, N W B A L; Ranathunge, R M T B; Amarasinghe, L D; Abeyewickreme, W

    2017-11-23

    Larval diet quality and rearing conditions have a direct and irreversible effect on adult traits. Therefore, the current study was carried out to optimize the larval diet for mass rearing of Aedes aegypti, for Sterile Insect Technique (SIT)-based applications in Sri Lanka. Five batches of 750 first instar larvae (L 1) of Ae. aegypti were exposed to five different concentrations (2-10%) of International Atomic Energy Agency (IAEA) recommended the larval diet. Morphological development parameters of larva, pupa, and adult were detected at 24 h intervals along with selected growth parameters. Each experiment was replicated five times. General Linear Modeling along with Pearson's correlation analysis were used for statistical treatments. Significant differences (P rate and success, sex ratio, adult success, fecundity and hatching rate of Ae. aegypti. The best quality adults can be produced at larval diet concentration of 10%. However, the 8% larval diet concentration was most suitable for adult male survival.

  7. PENGAMATAN TEMPAT PERINDUKAN AEDES AEGYPTI PADA TEMPAT PENAMPUNGAN AIR RUMAH TANGGA PADA MASYARAKAT PENGGUNA AIR OLAHAN

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    H. Hasyimi

    2012-11-01

    Full Text Available An observation of Aedes aegypti breeding places in domestic pipe waters container provided by PAM (Water supply company customer regency was carried out. The area observation in RW (a hamlet 05 kelurahan Papanggo Tanjung priuk North Jakarta. As a control area, RW 04 kelurahan Tanjung Priok in the same district was selected. The observation was conducted on Agust - September 2001. The result showed that Aedes aegypti larvae were found mostly in clay water container or tempayan (66,7 %. The house index (HI rate is 27,3%. In the control area the larvae were found predominantly in bath cistern (65,4% and HI rate is 100%. So in the study area HI rate is lower than in the control area.   Keywords: Aedes aegypti, breeding places, domestic container, house index

  8. First record of natural vertical transmission of dengue virus in Aedes aegypti from Cuba.

    Science.gov (United States)

    Gutiérrez-Bugallo, Gladys; Rodriguez-Roche, Rosmari; Díaz, Gisell; Vázquez, Antonio A; Alvarez, Mayling; Rodríguez, Magdalena; Bisset, Juan A; Guzman, Maria G

    2017-10-01

    While horizontal transmission (human-mosquito-human) of dengue viruses largely determines the epidemiology of the disease, vertical transmission (infected female mosquito- infected offspring) has been suggested as a mechanism that ensures maintenance of the virus during adverse conditions for horizontal transmission to occur. The purpose of this study was to analyze the natural infection of larval stages of Aedes aegypti (Diptera: Culicidae) with the dengue virus (DENV) in Cuba. Here, we report vertical transmission of DENV-3 genotype III in natural populations of Ae. aegypti through RT-PCR detection and serotyping plus sequencing. Our report constitutes the first record of vertical transmission of DENV in Ae. aegypti from Cuba with details of its serotype and genotype. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Relative Insecticidal Efficacy of Three Spatial Repellent Integrated Light Sources Against Aedes aegypti.

    Science.gov (United States)

    Shen, Yuan; Xue, Rui-De; Bibbs, Christopher S

    2017-12-01

    Three repellent products, OFF! Mosquito Lamp, Insecticandel, and Rescue DecoShield, were comparatively evaluated against Aedes aegypti in 130-m 2 enclosed areas with a 317-m 3 air volume. The results showed that the OFF! Mosquito Lamp with metofluthrin had a greater effect than the Insecticandel with transfluthrin, which had greater effect than the DecoShield with lemongrass oil and several other plant oils against Ae. aegypti. The OFF! Mosquito Lamp was the only product to exceed 50% mortality. An outdoor semi-field evaluation was conducted to determine the effect by distance of the product. Mosquitoes were stationed in cages at 3, 6, 9, 12, and 15 m away from the treatment in a downwind linear array and exposed for 10 min. They were recorded for knockdown after treatment and at 24 h for mortality. The OFF! Mosquito Lamp produced 100% mortality indoors and >80% knockdown and 90% mortality within 6 m while outdoors against Ae. aegypti.

  10. Novel Wolbachia-transinfected Aedes aegypti mosquitoes possess diverse fitness and vector competence phenotypes.

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    Johanna E Fraser

    2017-12-01

    Full Text Available Wolbachia pipientis from Drosophila melanogaster (wMel is an endosymbiotic bacterium that restricts transmission of human pathogenic flaviviruses and alphaviruses, including dengue, Zika, and chikungunya viruses, when introduced into the mosquito vector Aedes aegypti. To date, wMel-infected Ae. aegypti have been released in field trials in 5 countries to evaluate the effectiveness of this strategy for disease control. Despite the success in establishing wMel-infected mosquitoes in wild populations, and the well-characterized antiviral capabilities of wMel, transinfecting different or additional Wolbachia strains into Ae. aegypti may improve disease impact, and perhaps more importantly, could provide a strategy to account for the possible evolution of resistant arboviruses. Here, we report the successful transinfection of Ae. aegypti with the Wolbachia strains wMelCS (D. melanogaster, wRi (D. simulans and wPip (Culex quinquefasciatus and assess the effects on Ae. aegypti fitness, cytoplasmic incompatibility, tissue tropism and pathogen blocking in a laboratory setting. The results demonstrate that wMelCS provides a similar degree of protection against dengue virus as wMel following an infectious blood meal, and significantly reduces viral RNA levels beyond that of wMel following a direct challenge with infectious virus in mosquitoes, with no additional fitness cost to the host. The protection provided by wRi is markedly weaker than that of wMelCS, consistent with previous characterisations of these lines in Drosophila, while wPip was found to substantially reduce the fitness of Ae. aegypti. Thus, we determine wMelCS as a key candidate for further testing in field-relevant fitness tests and viremic blood feeding challenges in a clinical setting to determine if it may represent an alternative Wolbachia strain with more desirable attributes than wMel for future field testing.

  11. Novel Wolbachia-transinfected Aedes aegypti mosquitoes possess diverse fitness and vector competence phenotypes.

    Science.gov (United States)

    Fraser, Johanna E; De Bruyne, Jyotika Taneja; Iturbe-Ormaetxe, Iñaki; Stepnell, Justin; Burns, Rhiannon L; Flores, Heather A; O'Neill, Scott L

    2017-12-01

    Wolbachia pipientis from Drosophila melanogaster (wMel) is an endosymbiotic bacterium that restricts transmission of human pathogenic flaviviruses and alphaviruses, including dengue, Zika, and chikungunya viruses, when introduced into the mosquito vector Aedes aegypti. To date, wMel-infected Ae. aegypti have been released in field trials in 5 countries to evaluate the effectiveness of this strategy for disease control. Despite the success in establishing wMel-infected mosquitoes in wild populations, and the well-characterized antiviral capabilities of wMel, transinfecting different or additional Wolbachia strains into Ae. aegypti may improve disease impact, and perhaps more importantly, could provide a strategy to account for the possible evolution of resistant arboviruses. Here, we report the successful transinfection of Ae. aegypti with the Wolbachia strains wMelCS (D. melanogaster), wRi (D. simulans) and wPip (Culex quinquefasciatus) and assess the effects on Ae. aegypti fitness, cytoplasmic incompatibility, tissue tropism and pathogen blocking in a laboratory setting. The results demonstrate that wMelCS provides a similar degree of protection against dengue virus as wMel following an infectious blood meal, and significantly reduces viral RNA levels beyond that of wMel following a direct challenge with infectious virus in mosquitoes, with no additional fitness cost to the host. The protection provided by wRi is markedly weaker than that of wMelCS, consistent with previous characterisations of these lines in Drosophila, while wPip was found to substantially reduce the fitness of Ae. aegypti. Thus, we determine wMelCS as a key candidate for further testing in field-relevant fitness tests and viremic blood feeding challenges in a clinical setting to determine if it may represent an alternative Wolbachia strain with more desirable attributes than wMel for future field testing.

  12. Insecticide susceptibility of Aedes aegypti populations from Senegal and Cape Verde Archipelago.

    Science.gov (United States)

    Dia, Ibrahima; Diagne, Cheikh Tidiane; Ba, Yamar; Diallo, Diawo; Konate, Lassana; Diallo, Mawlouth

    2012-10-22

    Two concomitant dengue 3 (DEN-3) epidemics occurred in Cape Verde Archipelago and Senegal between September and October 2009. Aedes aegypti was identified as the vector of these epidemics as several DEN-3 virus strains were isolated from this species in both countries. The susceptibility to pyrethroids, organochlorine, organophosphates and carbamate was investigated in two field strains of Aedes aegypti from both countries using WHO diagnostic bioassay kits in order to monitor their the current status of insecticide susceptibility. The two tested strains were highly resistant to DDT. The Cape Verde strain was found to be susceptible to all others tested insecticides except for propoxur 0.1%, which needs further investigation. The Dakar strain was susceptible to fenitrothion 1% and permethrin 0.75%, but displayed reduced susceptibility to deltamethrin, lambda-cyhalothrin and propoxur. As base-line results, our observations stress a careful management of insecticide use for the control of Ae. aegypti. Indeed, they indicate that DDT is no longer efficient for the control of Ae. aegypti populations in Cape Verde and Dakar and further suggest a thorough follow-up of propoxur susceptibility status in both sites and that of deltamethrin and lambda-cyhalothrin in Ae. aegypti populations in Dakar. Thus, regular monitoring of susceptibility is greatly needed as well as the knowing if this observed resistance/susceptibility is focal or not and for observed resistance, the use of biochemical methods is needed with detailed comparison of resistance levels over a large geographic area. Aedes aegypti, Insecticides, Susceptibility, Cape Verde, Senegal.

  13. Contact irritant responses of Aedes aegypti Using sublethal concentration and focal application of pyrethroid chemicals.

    Directory of Open Access Journals (Sweden)

    Hortance Manda

    Full Text Available BACKGROUND: Previous studies have demonstrated contact irritant and spatial repellent behaviors in Aedes aegypti following exposure to sublethal concentrations of chemicals. These sublethal actions are currently being evaluated in the development of a push-pull strategy for Ae. aegypti control. This study reports on mosquito escape responses after exposure to candidate chemicals for a contact irritant focused push-pull strategy using varying concentrations and focal application. METHODS: Contact irritancy (escape behavior, knockdown and 24 hour mortality rates were quantified in populations of female Ae. aegypti under laboratory conditions and validated in the field (Thailand and Peru using experimental huts. Evaluations were conducted using varying concentrations and treatment surface area coverage (SAC of three pyrethroid insecticides: alphacypermethrin, lambacyhalothrin and deltamethrin. RESULTS: Under laboratory conditions, exposure of Ae. aegypti to alphacypermethrin using the standard field application rate (FAR resulted in escape responses at 25% and 50% SAC that were comparable with escape responses at 100% SAC. Significant escape responses were also observed at <100% SAC using ½FAR of all test compounds. In most trials, KD and 24 hour mortality rates were higher in mosquitoes that did not escape than in those that escaped. In Thailand, field validation studies indicated an early time of exit (by four hours and 40% increase in escape using ½FAR of alphacypermethrin at 75% SAC compared to a matched chemical-free control. In Peru, however, the maximum increase in Ae. aegypti escape from alphacypermethrin-treated huts was 11%. CONCLUSIONS/SIGNIFICANCE: Results presented here suggest a potential role for sublethal and focal application of contact irritant chemicals in an Ae. aegypti push-pull strategy to reduce human-vector contact inside treated homes. However, the impact of an increase in escape response on dengue virus transmission is

  14. Contact irritant responses of Aedes aegypti Using sublethal concentration and focal application of pyrethroid chemicals.

    Science.gov (United States)

    Manda, Hortance; Shah, Pankhil; Polsomboon, Suppaluck; Chareonviriyaphap, Theeraphap; Castro-Llanos, Fanny; Morrison, Amy; Burrus, Roxanne G; Grieco, John P; Achee, Nicole L

    2013-01-01

    Previous studies have demonstrated contact irritant and spatial repellent behaviors in Aedes aegypti following exposure to sublethal concentrations of chemicals. These sublethal actions are currently being evaluated in the development of a push-pull strategy for Ae. aegypti control. This study reports on mosquito escape responses after exposure to candidate chemicals for a contact irritant focused push-pull strategy using varying concentrations and focal application. Contact irritancy (escape) behavior, knockdown and 24 hour mortality rates were quantified in populations of female Ae. aegypti under laboratory conditions and validated in the field (Thailand and Peru) using experimental huts. Evaluations were conducted using varying concentrations and treatment surface area coverage (SAC) of three pyrethroid insecticides: alphacypermethrin, lambacyhalothrin and deltamethrin. Under laboratory conditions, exposure of Ae. aegypti to alphacypermethrin using the standard field application rate (FAR) resulted in escape responses at 25% and 50% SAC that were comparable with escape responses at 100% SAC. Significant escape responses were also observed at time of exit (by four hours) and 40% increase in escape using ½FAR of alphacypermethrin at 75% SAC compared to a matched chemical-free control. In Peru, however, the maximum increase in Ae. aegypti escape from alphacypermethrin-treated huts was 11%. Results presented here suggest a potential role for sublethal and focal application of contact irritant chemicals in an Ae. aegypti push-pull strategy to reduce human-vector contact inside treated homes. However, the impact of an increase in escape response on dengue virus transmission is currently unknown and will depend on rate of biting on human hosts prior to house exiting.

  15. Characteristics of Aedes aegypti adult mosquitoes in rural and urban areas of western and coastal Kenya

    Science.gov (United States)

    Ndenga, Bryson Alberto; Mutuku, Francis Maluki; Ngugi, Harun Njenga; Mbakaya, Joel Omari; Aswani, Peter; Musunzaji, Peter Siema; Vulule, John; Mukoko, Dunstan; Kitron, Uriel; LaBeaud, Angelle Desiree

    2017-01-01

    Aedes aegypti is the main vector for yellow fever, dengue, chikungunya and Zika viruses. Recent outbreaks of dengue and chikungunya have been reported in Kenya. Presence and abundance of this vector is associated with the risk for the occurrence and transmission of these diseases. This study aimed to characterize the presence and abundance of Ae. aegypti adult mosquitoes from rural and urban sites in western and coastal regions of Kenya. Presence and abundance of Ae. aegypti adult mosquitoes were determined indoors and outdoors in two western (urban Kisumu and rural Chulaimbo) and two coastal (urban Ukunda and rural Msambweni) sites in Kenya. Sampling was performed using quarterly human landing catches, monthly Prokopack automated aspirators and monthly Biogents-sentinel traps. A total of 2,229 adult Ae. aegypti mosquitoes were collected: 785 (35.2%) by human landing catches, 459 (20.6%) by Prokopack aspiration and 985 (44.2%) by Biogents-sentinel traps. About three times as many Ae. aegypti mosquitoes were collected in urban than rural sites (1,650 versus 579). Comparable numbers were collected in western (1,196) and coastal (1,033) sites. Over 80% were collected outdoors through human landing catches and Prokopack aspiration. The probability of collecting Ae. aegypti mosquitoes by human landing catches was significantly higher in the afternoon than morning hours (Paegypti mosquitoes were collected using Prokopack aspiration outdoors than indoors (Paegypti bites was highest in urban areas, outdoors and in the afternoon hours. These characteristics have major implications for the possible transmission of arboviral diseases and for the planning of surveillance and control programs. PMID:29261766

  16. Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability.

    Directory of Open Access Journals (Sweden)

    Sarah M Short

    2017-07-01

    Full Text Available The mosquito midgut microbiota has been shown to influence vector competence for multiple human pathogens. The microbiota is highly variable in the field, and the sources of this variability are not well understood, which limits our ability to understand or predict its effects on pathogen transmission. In this work, we report significant variation in female adult midgut bacterial load between strains of A. aegypti which vary in their susceptibility to dengue virus. Composition of the midgut microbiome was similar overall between the strains, with 81-92% of reads coming from the same five bacterial families, though we did detect differences in the presence of some bacterial families including Flavobacteriaceae and Entobacteriaceae. We conducted transcriptomic analysis on the two mosquito strains that showed the greatest difference in bacterial load, and found that they differ in transcript abundance of many genes implicated in amino acid metabolism, in particular the branched chain amino acid degradation pathway. We then silenced this pathway by targeting multiple genes using RNA interference, which resulted in strain-specific bacterial proliferation, thereby eliminating the difference in midgut bacterial load between the strains. This suggests that the branched chain amino acid (BCAA degradation pathway controls midgut bacterial load, though the mechanism underlying this remains unclear. Overall, our results indicate that amino acid metabolism can act to influence the midgut microbiota. Moreover, they suggest that genetic or physiological variation in BCAA degradation pathway activity may in part explain midgut microbiota variation in the field.

  17. Citrus Seed Oils Efficacy against Larvae of Aedes aegypti

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    Hazrat Bilal

    2017-10-01

    Full Text Available Background: Dengue fever is a serious public health issue in Pakistan for many years. Globally plants have been reported to contain compounds with insecticidal properties. These properties have been demonstrated more recently on the larval stages of mosquitoes. Therefore, Citrus cultivar seeds were evaluated for larvicidal potential against the primary dengue vector Aedes aegypti.Methods: Extraction of oil was done by a steam distillation method and oils were evaluated according to WHO guidelines for larvicides 2005 for evaluation of insecticidal properties of citrus seed extracts against mosquito larvae.Result: Among the Citrus cultivar seed oil, rough lemon (Citrus jambhiri had the lowest LC50 value (200.79ppm, while musambi (C. sinensis var musambi had the highest LC50 value (457.30ppm after 24 h of exposure.Conclusion: Citrus cultivars have some larvicidal potential but C. jambhiri had the greatest potential against A. ae­gypti larvae. Further small-scale field trials using the extracts of C. jambhiri will be conducted to determine opera­tional feasibility.

  18. Horizontal gene transfer between Wolbachia and the mosquito Aedes aegypti

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    Walker Thomas

    2009-01-01

    Full Text Available Abstract Background The evolutionary importance of horizontal gene transfer (HGT from Wolbachia endosymbiotic bacteria to their eukaryotic hosts is a topic of considerable interest and debate. Recent transfers of genome fragments from Wolbachia into insect chromosomes have been reported, but it has been argued that these fragments may be on an evolutionary trajectory to degradation and loss. Results We have discovered a case of HGT, involving two adjacent genes, between the genomes of Wolbachia and the currently Wolbachia-uninfected mosquito Aedes aegypti, an important human disease vector. The lower level of sequence identity between Wolbachia and insect, the transcription of all the genes involved, and the fact that we have identified homologs of the two genes in another Aedes species (Ae. mascarensis, suggest that these genes are being expressed after an extended evolutionary period since horizontal transfer, and therefore that the transfer has functional significance. The association of these genes with Wolbachia prophage regions also provides a mechanism for the transfer. Conclusion The data support the argument that HGT between Wolbachia endosymbiotic bacteria and their hosts has produced evolutionary innovation.

  19. Modulation of Host Learning in Aedes aegypti Mosquitoes.

    Science.gov (United States)

    Vinauger, Clément; Lahondère, Chloé; Wolff, Gabriella H; Locke, Lauren T; Liaw, Jessica E; Parrish, Jay Z; Akbari, Omar S; Dickinson, Michael H; Riffell, Jeffrey A

    2018-02-05

    How mosquitoes determine which individuals to bite has important epidemiological consequences. This choice is not random; most mosquitoes specialize in one or a few vertebrate host species, and some individuals in a host population are preferred over others. Mosquitoes will also blood feed from other hosts when their preferred is no longer abundant, but the mechanisms mediating these shifts between hosts, and preferences for certain individuals within a host species, remain unclear. Here, we show that olfactory learning may contribute to Aedes aegypti mosquito biting preferences and host shifts. Training and testing to scents of humans and other host species showed that mosquitoes can aversively learn the scent of specific humans and single odorants and learn to avoid the scent of rats (but not chickens). Using pharmacological interventions, RNAi, and CRISPR gene editing, we found that modification of the dopamine-1 receptor suppressed their learning abilities. We further show through combined electrophysiological and behavioral recordings from tethered flying mosquitoes that these odors evoke changes in both behavior and antennal lobe (AL) neuronal responses and that dopamine strongly modulates odor-evoked responses in AL neurons. Not only do these results provide direct experimental evidence that olfactory learning in mosquitoes can play an epidemiological role, but collectively, they also provide neuroanatomical and functional demonstration of the role of dopamine in mediating this learning-induced plasticity, for the first time in a disease vector insect. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Lipídios em sedimentos arqueológicos: resultados preliminares do sítio arqueológico Rio do Meio, Ilha de Santa Catarina (SC Lipids in archaeological sediments: preliminary results of the archaeological site Rio do Meio, Santa Catarina Island (SC, Brazil

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    Fabrício Augusto Hansel

    2008-02-01

    Full Text Available Este trabalho foi realizado com o objetivo de avaliar a distribuição de lipídios em sedimentos arqueológicos do sítio Rio do Meio, Ilha de Santa Catarina. Nos extratos totais de lipídios, analisados por cromatografia gasosa e espectrometria de massas (CG e CG-EM, predominaram os compostos ácidos e álcoois saturados. Foram detectados pelo menos dois tipos de matéria orgânica: uma antiga e outra comparativamente recente. Na primeira, o extrato total de lipídios foi dominado por ácidos graxos de cadeias curtas ( Ac20:0 e Al20:0. Em contraste, na deposição classificada como recente, foram identificados em maiores teores os ácidos e álcoois de cadeias longas (> Ac20:0 e Al20:0. Neste estudo, foi possível observar a incorporação de material orgânico procedente de fontes de origem vegetal, bacteriana e, possivelmente, animal (gorduras nos sedimentos arqueológicos analisados.In this study the distribution of lipid compounds was evaluated in sediment samples of an archaeological site Rio do Meio, Santa Catarina Island, Brazil. In the total lipid extracts, analyzed by gas chromatography and mass spectrometry (GC and GC-MS, saturated fatty acids and alcohols were predominant. At least two sources of organic matter were detected, an older and a more recent one. In the old deposit, the most abundant lipids were short-chain fatty acids ( Ac20:0 and Al20:0. In contrast, the fresh deposit was dominated by long-chain fatty acids and alcohols (> Ac20:0 and Al20:0. This paper described the incorporation of vegetal, bacterial and possible animal (fat sources into the archaeological sediments analyzed.

  1. Characteristics of Aedes aegypti adult mosquitoes in rural and urban areas of western and coastal Kenya.

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    Bryson Alberto Ndenga

    Full Text Available Aedes aegypti is the main vector for yellow fever, dengue, chikungunya and Zika viruses. Recent outbreaks of dengue and chikungunya have been reported in Kenya. Presence and abundance of this vector is associated with the risk for the occurrence and transmission of these diseases. This study aimed to characterize the presence and abundance of Ae. aegypti adult mosquitoes from rural and urban sites in western and coastal regions of Kenya. Presence and abundance of Ae. aegypti adult mosquitoes were determined indoors and outdoors in two western (urban Kisumu and rural Chulaimbo and two coastal (urban Ukunda and rural Msambweni sites in Kenya. Sampling was performed using quarterly human landing catches, monthly Prokopack automated aspirators and monthly Biogents-sentinel traps. A total of 2,229 adult Ae. aegypti mosquitoes were collected: 785 (35.2% by human landing catches, 459 (20.6% by Prokopack aspiration and 985 (44.2% by Biogents-sentinel traps. About three times as many Ae. aegypti mosquitoes were collected in urban than rural sites (1,650 versus 579. Comparable numbers were collected in western (1,196 and coastal (1,033 sites. Over 80% were collected outdoors through human landing catches and Prokopack aspiration. The probability of collecting Ae. aegypti mosquitoes by human landing catches was significantly higher in the afternoon than morning hours (P<0.001, outdoors than indoors (P<0.001 and in urban than rural sites (P = 0.008. Significantly more Ae. aegypti mosquitoes were collected using Prokopack aspiration outdoors than indoors (P<0.001 and in urban than rural areas (P<0.001. Significantly more mosquitoes were collected using Biogents-sentinel traps in urban than rural areas (P = 0.008 and in western than coastal sites (P = 0.006. The probability of exposure to Ae. aegypti bites was highest in urban areas, outdoors and in the afternoon hours. These characteristics have major implications for the possible transmission of arboviral

  2. Vector competence of populations of Aedes aegypti from three distinct cities in Kenya for chikungunya virus.

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    Sheila B Agha

    2017-08-01

    Full Text Available In April, 2004, chikungunya virus (CHIKV re-emerged in Kenya and eventually spread to the islands in the Indian Ocean basin, South-East Asia, and the Americas. The virus, which is often associated with high levels of viremia in humans, is mostly transmitted by the urban vector, Aedes aegypti. The expansion of CHIKV presents a public health challenge both locally and internationally. In this study, we investigated the ability of Ae. aegypti mosquitoes from three distinct cities in Kenya; Mombasa (outbreak prone, Kisumu, and Nairobi (no documented outbreak to transmit CHIKV.Aedes aegypti mosquito populations were exposed to different doses of CHIKV (105.6-7.5 plaque-forming units[PFU]/ml in an infectious blood meal. Transmission was ascertained by collecting and testing saliva samples from individual mosquitoes at 5, 7, 9, and 14 days post exposure. Infection and dissemination were estimated by testing body and legs, respectively, for individual mosquitoes at selected days post exposure. Tissue culture assays were used to determine the presence of infectious viral particles in the body, leg, and saliva samples. The number of days post exposure had no effect on infection, dissemination, or transmission rates, but these rates increased with an increase in exposure dose in all three populations. Although the rates were highest in Ae. aegypti from Mombasa at titers ≥106.9 PFU/ml, the differences observed were not statistically significant (χ2 ≤ 1.04, DF = 1, P ≥ 0.31. Overall, about 71% of the infected mosquitoes developed a disseminated infection, of which 21% successfully transmitted the virus into a capillary tube, giving an estimated transmission rate of about 10% for mosquitoes that ingested ≥106.9 PFU/ml of CHIKV. All three populations of Ae. aegypti were infectious as early as 5-7 days post exposure. On average, viral dissemination only occurred when body titers were ≥104 PFU/ml in all populations.Populations of Ae. aegypti from

  3. Vector competence of populations of Aedes aegypti from three distinct cities in Kenya for chikungunya virus.

    Science.gov (United States)

    Agha, Sheila B; Chepkorir, Edith; Mulwa, Francis; Tigoi, Caroline; Arum, Samwel; Guarido, Milehna M; Ambala, Peris; Chelangat, Betty; Lutomiah, Joel; Tchouassi, David P; Turell, Michael J; Sang, Rosemary

    2017-08-01

    In April, 2004, chikungunya virus (CHIKV) re-emerged in Kenya and eventually spread to the islands in the Indian Ocean basin, South-East Asia, and the Americas. The virus, which is often associated with high levels of viremia in humans, is mostly transmitted by the urban vector, Aedes aegypti. The expansion of CHIKV presents a public health challenge both locally and internationally. In this study, we investigated the ability of Ae. aegypti mosquitoes from three distinct cities in Kenya; Mombasa (outbreak prone), Kisumu, and Nairobi (no documented outbreak) to transmit CHIKV. Aedes aegypti mosquito populations were exposed to different doses of CHIKV (105.6-7.5 plaque-forming units[PFU]/ml) in an infectious blood meal. Transmission was ascertained by collecting and testing saliva samples from individual mosquitoes at 5, 7, 9, and 14 days post exposure. Infection and dissemination were estimated by testing body and legs, respectively, for individual mosquitoes at selected days post exposure. Tissue culture assays were used to determine the presence of infectious viral particles in the body, leg, and saliva samples. The number of days post exposure had no effect on infection, dissemination, or transmission rates, but these rates increased with an increase in exposure dose in all three populations. Although the rates were highest in Ae. aegypti from Mombasa at titers ≥106.9 PFU/ml, the differences observed were not statistically significant (χ2 ≤ 1.04, DF = 1, P ≥ 0.31). Overall, about 71% of the infected mosquitoes developed a disseminated infection, of which 21% successfully transmitted the virus into a capillary tube, giving an estimated transmission rate of about 10% for mosquitoes that ingested ≥106.9 PFU/ml of CHIKV. All three populations of Ae. aegypti were infectious as early as 5-7 days post exposure. On average, viral dissemination only occurred when body titers were ≥104 PFU/ml in all populations. Populations of Ae. aegypti from Mombasa, Nairobi

  4. Methods for TALEN Evaluation, Use, and Mutation Detection in the Mosquito Aedes aegypti.

    Science.gov (United States)

    Basu, Sanjay; Aryan, Azadeh; Haac, Mary Etna; Myles, Kevin M; Adelman, Zach N

    2016-01-01

    The generation and study of transgenic Aedes aegypti mosquitoes provides an essential tool for elucidating the complex molecular biology of this important vector. Within the field, genetic manipulation has surpassed the proof of principle stage and is now utilized in both applied and theoretical vector control strategies. The application of new instruments, technologies and techniques allows ever more controlled experiments to be conducted. In this text we describe microinjection of Ae. aegypti embryos in the context of evaluating and performing genomic editing with transcription activator-like effector nucleases (TALENs).

  5. Geographic distribution of Aedes aegypti and Aedes albopictus collected from used tires in Vietnam.

    Science.gov (United States)

    Higa, Yukiko; Yen, Nguyen Thi; Kawada, Hitoshi; Son, Tran Hai; Hoa, Nguyen Thuy; Takagi, Masahiro

    2010-03-01

    The spatial distribution of Aedes aegypti and Aedes albopictus in environmental and geographical zones, e.g., urban-rural, coastal-mountainous, and north-south, was investigated throughout Vietnam. Immature stages were collected from used tires along roads. The effects of regions, seasons, and the degree of urbanization on the density and the frequency were statistically analyzed. Aedes aegypti predominated in the southern and central regions, while Ae. albopictus predominated in the northern region, which may be related to climatic conditions (temperature and rainfall). Larval collection from used tires may be suitable to assess rapidly the current distribution of dengue mosquitoes for estimating health risks and implementing vector control measures.

  6. History of domestication and spread of Aedes aegypti--a review.

    Science.gov (United States)

    Powell, Jeffrey R; Tabachnick, Walter J

    2013-01-01

    The adaptation of insect vectors of human diseases to breed in human habitats (domestication) is one of the most important phenomena in medical entomology. Considerable data are available on the vector mosquito Aedes aegypti in this regard and here we integrate the available information including genetics, behaviour, morphology, ecology and biogeography of the mosquito, with human history. We emphasise the tremendous amount of variation possessed by Ae. aegypti for virtually all traits considered. Typological thinking needs to be abandoned to reach a realistic and comprehensive understanding of this important vector of yellow fever, dengue and Chikungunya.

  7. Cruzamiento interespecífico entre Aedes aegypti y Aedes albopictus en el laboratorio

    OpenAIRE

    Martínez López, Yanisley; Martinez Pérez, Yanet; Acosta Rodríguez, Miriam; Fuentes González, Omar

    2014-01-01

    Introducción: existen algunos estudios realizados para verificar el posible apareamiento interespecífico, pero solo algunos trabajos han obtenido resultados positivos en este fenómeno. Objetivo: probar la posibilidad de obtener huevos viables del cruce entre Aedes aegypti y Aedes albopictus. Métodos: experimentos de apareamiento recíproco entre Aedes aegypti procedentes del insectario del Departamento de Control de Vectores del Instituto de Medicina Tropical "Pedro Kourí" y una población de A...

  8. Intermolecular interaction of thiosemicarbazone derivatives to solvents and a potential Aedes aegypti target

    Science.gov (United States)

    da Silva, João Bosco P.; Hallwass, Fernando; da Silva, Aluizio G.; Moreira, Diogo Rodrigo; Ramos, Mozart N.; Espíndola, José Wanderlan P.; de Oliveira, Ana Daura T.; Brondani, Dalci José; Leite, Ana Cristina L.; Merz, Kenneth M.

    2015-08-01

    DFT calculations were used to access information about structure, energy and electronic properties of series of phenyl- and phenoxymethyl-(thio)semicarbazone derivatives with demonstrated activity against the larvae of Aedes aegypti in stage L4. The way as the thiosemicarbazone derivatives can interact with solvents like DMSO and water were analyzed from the comparison between calculated and experimental 1H NMR chemical shifts. The evidences of thiosemicarbazone derivatives making H-bond interaction to solvent have provide us insights on how they can interact with a potential A. aegypti's biological target, the Sterol Carrier Protein-2.

  9. Comparison of the diet of Alouatta caraya (Primates: Atelidae between a riparian island and mainland on the Upper Parana River, southern Brazil Comparação da dieta de Alouatta caraya (Primates: Atelidae em mata ciliar insular e continental na região do Alto rio Paraná, Sul do Brasil

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    Gabriela Ludwig

    2008-09-01

    Full Text Available Howler monkeys (Alouatta Lacèpéde, 1799 are folivores-frugivores with flexible diets depending on conditions. Here, we compare the diets of Alouatta caraya (Humboldt, 1812 in two riparian forests (island and mainland, in Porto Rico region, Upper Paraná River, Southern Brazil. Howlers were followed from October 2004 to September 2005 in the riparian forest of a 1,050 ha island and in the continuous riparian forest on the mainland (left bank of the river. The "scan sampling" method with instantaneous samples every 15 minutes was used. Besides vines, diet breadth was similar: 17 species consumed on the island versus 16 species on the mainland. Both consumed leaves followed by fruits more than any other food type (leaves: island - 65%, mainland - 49%, fruits: island - 24%, mainland - 46%. Even though the plant Cecropia pachystachya Trécul is less abundant in the mainland it was the item most consumed in both locations all year long, which suggests its importance for the howlers. Diet also varied both seasonally and between the island and mainland, apparently following changes in local abundance of each item and due to plant phenologies.Os bugios (Alouatta Lacèpéde, 1799 são primatas folívoros-frugívoros que podem adaptar suas dietas dependendo das condições do ambiente. O presente trabalho compara a dieta de grupos de Alouatta caraya (Humboldt, 1812 presentes em matas ciliares estruturalmente diferentes (ilha e continente, na região de Porto Rico, Alto rio Paraná, sul do Brasil. Os bugios-pretos foram seguidos de outubro de 2004 a setembro de 2005 em mata ciliar de uma ilha fluvial de 1050 ha e em mata ciliar contínua da margem esquerda do rio. Foi utilizado o método "scan sampling" em amostragens instantâneas com intervalos de 15 minutos. Além das lianas, 17 espécies foram consumidas na ilha versus 16 espécies no continente. Ambos os grupos consumiram mais folhas seguido de frutos do que qualquer outro tipo de alimento (folhas

  10. Evaluación del efecto residual del temephos en larvas de Aedes aegypti en Lima, Perú

    OpenAIRE

    Palomino S, Miriam; Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú.; Solari, Lely; Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Hospital Nacional Hipólito Unánue. Lima, Perú.; León C, Walter; Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú.; Vega H, Rosario; Centro Nacional de Control de Calidad, Instituto Nacional de Salud. Lima, Perú.; Vergaray C, Máximo; Centro Nacional de Control de Calidad, Instituto Nacional de Salud. Lima, Perú.; Cubillas, Luis; Dirección de Salud Lima Norte III, Ministerio de Salud. Lima, Perú.; Mosqueda C, Rosa; Centro Nacional de Control de Calidad, Instituto Nacional de Salud. Lima, Perú.; García A, Norma; Centro Nacional de Control de Calidad, Instituto Nacional de Salud. Lima, Perú.

    2006-01-01

    El temephos ha sido usado como la única estrategia de control para Aedes aegypti en Lima durante los últimos años. Objetivo: Evaluar la eficacia residual de temephos para el control de Ae. aegypti en condiciones de campo y laborato - rio en Lima, Perú. Materiales y métodos: Se eligieron ocho tanques bajos de concreto (TBC) depósitos predominan - temente infestados con Ae. aegypti en el distrito de San Juan de Lurigancho, situado al norte del área suburbana de Lima. Se cuantificó el número de ...

  11. Population genomics reveals that an anthropophilic population of Aedes aegypti mosquitoes in West Africa recently gave rise to American and Asian populations of this major disease vector.

    Science.gov (United States)

    Crawford, Jacob E; Alves, Joel M; Palmer, William J; Day, Jonathan P; Sylla, Massamba; Ramasamy, Ranjan; Surendran, Sinnathamby N; Black, William C; Pain, Arnab; Jiggins, Francis M

    2017-02-28

    The mosquito Aedes aegypti is the main vector of dengue, Zika, chikungunya and yellow fever viruses. This major disease vector is thought to have arisen when the African subspecies Ae. aegypti formosus evolved from being zoophilic and living in forest habitats into a form that specialises on humans and resides near human population centres. The resulting domestic subspecies, Ae. aegypti aegypti, is found throughout the tropics and largely blood-feeds on humans. To understand this transition, we have sequenced the exomes of mosquitoes collected from five populations from around the world. We found that Ae. aegypti specimens from an urban population in Senegal in West Africa were more closely related to populations in Mexico and Sri Lanka than they were to a nearby forest population. We estimate that the populations in Senegal and Mexico split just a few hundred years ago, and we found no evidence of Ae. aegypti aegypti mosquitoes migrating back to Africa from elsewhere in the tropics. The out-of-Africa migration was accompanied by a dramatic reduction in effective population size, resulting in a loss of genetic diversity and rare genetic variants. We conclude that a domestic population of Ae. aegypti in Senegal and domestic populations on other continents are more closely related to each other than to other African populations. This suggests that an ancestral population of Ae. aegypti evolved to become a human specialist in Africa, giving rise to the subspecies Ae. aegypti aegypti. The descendants of this population are still found in West Africa today, and the rest of the world was colonised when mosquitoes from this population migrated out of Africa. This is the first report of an African population of Ae. aegypti aegypti mosquitoes that is closely related to Asian and American populations. As the two subspecies differ in their ability to vector disease, their existence side by side in West Africa may have important implications for disease transmission.

  12. Population genomics reveals that an anthropophilic population of Aedes aegypti mosquitoes in West Africa recently gave rise to American and Asian populations of this major disease vector

    KAUST Repository

    Crawford, Jacob E.

    2017-02-20

    BackgroundThe mosquito Aedes aegypti is the main vector of dengue, Zika, chikungunya and yellow fever viruses. This major disease vector is thought to have arisen when the African subspecies Ae. aegypti formosus evolved from being zoophilic and living in forest habitats into a form that specialises on humans and resides near human population centres. The resulting domestic subspecies, Ae. aegypti aegypti, is found throughout the tropics and largely blood-feeds on humans.ResultsTo understand this transition, we have sequenced the exomes of mosquitoes collected from five populations from around the world. We found that Ae. aegypti specimens from an urban population in Senegal in West Africa were more closely related to populations in Mexico and Sri Lanka than they were to a nearby forest population. We estimate that the populations in Senegal and Mexico split just a few hundred years ago, and we found no evidence of Ae. aegypti aegypti mosquitoes migrating back to Africa from elsewhere in the tropics. The out-of-Africa migration was accompanied by a dramatic reduction in effective population size, resulting in a loss of genetic diversity and rare genetic variants.ConclusionsWe conclude that a domestic population of Ae. aegypti in Senegal and domestic populations on other continents are more closely related to each other than to other African populations. This suggests that an ancestral population of Ae. aegypti evolved to become a human specialist in Africa, giving rise to the subspecies Ae. aegypti aegypti. The descendants of this population are still found in West Africa today, and the rest of the world was colonised when mosquitoes from this population migrated out of Africa. This is the first report of an African population of Ae. aegypti aegypti mosquitoes that is closely related to Asian and American populations. As the two subspecies differ in their ability to vector disease, their existence side by side in West Africa may have important implications for

  13. Evolutionary enhancement of Zika virus infectivity in Aedes aegypti mosquitoes.

    Science.gov (United States)

    Liu, Yang; Liu, Jianying; Du, Senyan; Shan, Chao; Nie, Kaixiao; Zhang, Rudian; Li, Xiao-Feng; Zhang, Renli; Wang, Tao; Qin, Cheng-Feng; Wang, Penghua; Shi, Pei-Yong; Cheng, Gong

    2017-05-25

    Zika virus (ZIKV) remained obscure until the recent explosive outbreaks in French Polynesia (2013-2014) and South America (2015-2016). Phylogenetic studies have shown that ZIKV has evolved into African and Asian lineages. The Asian lineage of ZIKV was responsible for the recent epidemics in the Americas. However, the underlying mechanisms through which ZIKV rapidly and explosively spread from Asia to the Americas are unclear. Non-structural protein 1 (NS1) facilitates flavivirus acquisition by mosquitoes from an infected mammalian host and subsequently enhances viral prevalence in mosquitoes. Here we show that NS1 antigenaemia determines ZIKV infectivity in its mosquito vector Aedes aegypti, which acquires ZIKV via a blood meal. Clinical isolates from the most recent outbreak in the Americas were much more infectious in mosquitoes than the FSS13025 strain, which was isolated in Cambodia in 2010. Further analyses showed that these epidemic strains have higher NS1 antigenaemia than the FSS13025 strain because of an alanine-to-valine amino acid substitution at residue 188 in NS1. ZIKV infectivity was enhanced by this amino acid substitution in the ZIKV FSS13025 strain in mosquitoes that acquired ZIKV from a viraemic C57BL/6 mouse deficient in type I and II interferon (IFN) receptors (AG6 mouse). Our results reveal that ZIKV evolved to acquire a spontaneous mutation in its NS1 protein, resulting in increased NS1 antigenaemia. Enhancement of NS1 antigenaemia in infected hosts promotes ZIKV infectivity and prevalence in mosquitoes, which could have facilitated transmission during recent ZIKV epidemics.

  14. Vector Competence of French Polynesian Aedes aegypti and Aedes polynesiensis for Zika Virus.

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    Vaea Richard

    2016-09-01

    Full Text Available In 2013-2014, French Polynesia experienced for the first time a Zika outbreak. Two Aedes mosquitoes may have contributed to Zika virus (ZIKV transmission in French Polynesia: the worldwide distributed Ae. aegypti and the Polynesian islands-endemic Ae. polynesiensis mosquito.To evaluate their vector competence for ZIKV, mosquitoes were infected per os at viral titers of 7 logs tissue culture infectious dose 50%. At several days post-infection (dpi, saliva was collected from each mosquito and inoculated onto C6/36 mosquito cells to check for the presence of ZIKV infectious particles. Legs and body of each mosquito were also collected and submitted separately to RNA extraction and ZIKV RT-PCR. In Ae. aegypti the infection rate was high as early as 6 dpi and the dissemination efficiency get substantial from 9 dpi while the both rates remained quite low in Ae. polynesiensis. The transmission efficiency was poor in Ae. aegypti until 14 dpi and no infectious saliva was found in Ae. polynesiensis at the time points studied.In our experimental conditions, the late ability of the French Polynesian Ae. aegypti to transmit ZIKV added by the poor competence of Ae. polynesiensis for this virus suggest the possible contribution of another vector for the propagation of ZIKV during the outbreak, in particular in remote islands where Ae. polynesiensis is predominating.

  15. Ovicidal activity of Metarhizium brunneum (Mb F52) on dengue fever vector, Aedes aegypti

    Science.gov (United States)

    The ovicidal activity of Metarhizium brunneum F52 (Mb F52) grown from granules was evaluated against Aedes aegypti eggs over time. Survival of larvae from treated eggs was significantly less when compared with untreated eggs at 7, 10 and 14 days post treatment. Only 27 % of treated eggs produced vi...

  16. TALEN-based gene disruption in the dengue vector Aedes aegypti.

    Science.gov (United States)

    Aryan, Azadeh; Anderson, Michelle A E; Myles, Kevin M; Adelman, Zach N

    2013-01-01

    In addition to its role as the primary vector for dengue viruses, Aedes aegypti has a long history as a genetic model organism for other bloodfeeding mosquitoes, due to its ease of colonization, maintenance and reproductive productivity. Though its genome has been sequenced, functional characterization of many Ae. aegypti genes, pathways and behaviors has been slow. TALE nucleases (TALENs) have been used with great success in a number of organisms to generate site-specific DNA lesions. We evaluated the ability of a TALEN pair to target the Ae. aegypti kmo gene, whose protein product is essential in the production of eye pigmentation. Following injection into pre-blastoderm embryos, 20-40% of fertile survivors produced kmo alleles that failed to complement an existing kh(w) mutation. Most of these individuals produced more than 20% white-eyed progeny, with some producing up to 75%. Mutant alleles were associated with lesions of 1-7 bp specifically at the selected target site. White-eyed individuals could also be recovered following a blind intercross of G1 progeny, yielding several new white-eyed strains in the genetic background of the sequenced Liverpool strain. We conclude that TALENs are highly active in the Ae. aegypti germline, and have the potential to transform how reverse genetic experiments are performed in this important disease vector.

  17. First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas.

    Science.gov (United States)

    Costa-da-Silva, André Luis; Ioshino, Rafaella Sayuri; Petersen, Vivian; Lima, Antonio Fernando; Cunha, Marielton Dos Passos; Wiley, Michael R; Ladner, Jason T; Prieto, Karla; Palacios, Gustavo; Costa, Danuza Duarte; Suesdek, Lincoln; Zanotto, Paolo Marinho de Andrade; Capurro, Margareth Lara

    2017-06-01

    The worldwide expansion of new emergent arboviruses such as Chikungunya and Zika reinforces the importance in understanding the role of mosquito species in spreading these pathogens in affected regions. This knowledge is essential for developing effective programs based on species specificity to avoid the establishment of endemic transmission cycles sustained by the identified local vectors. Although the first autochthonous transmission of Chikungunya virus was described in 2014 in the north of Brazil, the main outbreaks were reported in 2015 and 2016 in the northeast of Brazil. During 5 days of February 2016, we collected mosquitoes in homes of 6 neighborhoods of Aracaju city, the capital of Sergipe state. Four mosquito species were identified but Culex quinquefasciatus and Aedes aegypti were the most abundant. Field-caught mosquitoes were tested for Chikungunya (CHIKV), Zika (ZIKV) and Dengue viruses (DENV) by qRT-PCR and one CHIKV-infected Ae. aegypti female was detected. The complete sequence of CHIKV genome was obtained from this sample and phylogenetic analysis revealed that this isolate belongs to the East-Central-South-African (ECSA) genotype. Our study describes the first identification of a naturally CHIKV-infected Ae. aegypti in Brazil and the first report of a CHIKV from ECSA genotype identified in this species in the Americas. These findings support the notion of Ae. aegypti being a vector involved in CHIKV outbreaks in northeast of Brazil.

  18. First Report of Aedes aegypti Transmission of Chikungunya Virus in the Americas.

    Science.gov (United States)

    Díaz-González, Esteban E; Kautz, Tiffany F; Dorantes-Delgado, Alicia; Malo-García, Iliana R; Laguna-Aguilar, Maricela; Langsjoen, Rose M; Chen, Rubing; Auguste, Dawn I; Sánchez-Casas, Rosa M; Danis-Lozano, Rogelio; Weaver, Scott C; Fernández-Salas, Ildefonso

    2015-12-01

    During a chikungunya fever outbreak in late 2014 in Chiapas, Mexico, entomovirological surveillance was performed to incriminate the vector(s). In neighborhoods, 75 households with suspected cases were sampled for mosquitoes, of which 80% (60) harbored Aedes aegypti and 2.7% (2) Aedes albopictus. A total of 1,170 Ae. aegypti and three Ae. albopictus was collected and 81 pools were generated. Although none of the Ae. albopictus pools were chikungunya virus (CHIKV)-positive, 18 Ae. aegypti pools (22.8%) contained CHIKV, yielding an infection rate of 32.3/1,000 mosquitoes. A lack of herd immunity in conjunction with high mosquito populations, poor vector control services in this region, and targeted collections in locations of human cases may explain the high infection rate in this vector. Consistent with predictions from experimental studies, Ae. aegypti appears to be the principal vector of CHIKV in southern Mexico, while the role of Ae. albopictus remains unknown. © The American Society of Tropical Medicine and Hygiene.

  19. [Susceptibility of Aedes aegypti (L.) strains from Havana to a Bacillus thuringiensis var. israelensis].

    Science.gov (United States)

    Menéndez Díaz, Zulema; Rodríguez Rodríguez, Jinnay; Gato Armas, René; Companioni Ibañez, Ariamys; Díaz Pérez, Manuel; Bruzón Aguila, Rosa Yirian

    2012-01-01

    the integration of chemical and biological methods is one of the strategies for the vector control, due to the existing environmental problems and the concerns of the community as a result of the synthetic organic insecticide actions. The bacterium called Bacillus thuringiensis var. israelensis in liquid formulation has been widely used in the vector control programs in several countries and has shown high efficacy at lab in Cuba. to determine the susceptibility of Aedes aegypti collected in the municipalities of La Habana province to Bacillus thuringiensis var. israelensis. fifteen Aedes aegypti strains, one from each municipality, were used including larvae and pupas collected in 2010 and one reference strain known as Rockefeller. The aqueous formulation of Bacillus thuringiensis var. israelensis (Bactivec, Labiofam, Cuba) was used. The bioassays complied with the World Health Organization guidelines for use of bacterial larvicides in the public health sector. The larval mortality was read after 24 hours and the results were processed by the statistical system SPSS (11.0) through Probit analysis. the evaluated mosquito strains showed high susceptibility to biolarvicide, there were no significant differences in LC50 values of Ae. aegypti strains, neither in the comparison of these values with those of the reference strain. the presented results indicate that the use of Bacillus thuringiensis var. israelensis continues to be a choice for the control of Aedes aegypti larval populations in La Habana province.

  20. Complex modulation of the Aedes aegypti transcriptome in response to dengue virus infection.

    Science.gov (United States)

    Bonizzoni, Mariangela; Dunn, W Augustine; Campbell, Corey L; Olson, Ken E; Marinotti, Osvaldo; James, Anthony A

    2012-01-01

    Dengue fever is the most important arboviral disease world-wide, with Aedes aegypti being the major vector. Interactions between the mosquito host and dengue viruses (DENV) are complex and vector competence varies among geographically-distinct Ae. aegypti populations. Additionally, dengue is caused by four antigenically-distinct viral serotypes (DENV1-4), each with multiple genotypes. Each virus genotype interacts differently with vertebrate and invertebrate hosts. Analyses of alterations in mosquito transcriptional profiles during DENV infection are expected to provide the basis for identifying networks of genes involved in responses to viruses and contribute to the molecular-genetic understanding of vector competence. In addition, this knowledge is anticipated to support the development of novel disease-control strategies. RNA-seq technology was used to assess genome-wide changes in transcript abundance at 1, 4 and 14 days following DENV2 infection in carcasses, midguts and salivary glands of the Ae. aegypti Chetumal strain. DENV2 affected the expression of 397 Ae. aegypti genes, most of which were down-regulated by viral infection. Differential accumulation of transcripts was mainly tissue- and time-specific. Comparisons of our data with other published reports reveal conservation of functional classes, but limited concordance of specific mosquito genes responsive to DENV2 infection. These results indicate the necessity of additional studies of mosquito-DENV interactions, specifically those focused on recently-derived mosquito strains with multiple dengue virus serotypes and genotypes.

  1. Complex modulation of the Aedes aegypti transcriptome in response to dengue virus infection.

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    Mariangela Bonizzoni

    Full Text Available Dengue fever is the most important arboviral disease world-wide, with Aedes aegypti being the major vector. Interactions between the mosquito host and dengue viruses (DENV are complex and vector competence varies among geographically-distinct Ae. aegypti populations. Additionally, dengue is caused by four antigenically-distinct viral serotypes (DENV1-4, each with multiple genotypes. Each virus genotype interacts differently with vertebrate and invertebrate hosts. Analyses of alterations in mosquito transcriptional profiles during DENV infection are expected to provide the basis for identifying networks of genes involved in responses to viruses and contribute to the molecular-genetic understanding of vector competence. In addition, this knowledge is anticipated to support the development of novel disease-control strategies. RNA-seq technology was used to assess genome-wide changes in transcript abundance at 1, 4 and 14 days following DENV2 infection in carcasses, midguts and salivary glands of the Ae. aegypti Chetumal strain. DENV2 affected the expression of 397 Ae. aegypti genes, most of which were down-regulated by viral infection. Differential accumulation of transcripts was mainly tissue- and time-specific. Comparisons of our data with other published reports reveal conservation of functional classes, but limited concordance of specific mosquito genes responsive to DENV2 infection. These results indicate the necessity of additional studies of mosquito-DENV interactions, specifically those focused on recently-derived mosquito strains with multiple dengue virus serotypes and genotypes.

  2. Semaphorin-1a is required for Aedes aegypti embryonic nerve cord development.

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    Morgan Haugen

    Full Text Available Although mosquito genome projects have uncovered orthologues of many known developmental regulatory genes, extremely little is known about mosquito development. In this study, the role of semaphorin-1a (sema1a was investigated during vector mosquito embryonic ventral nerve cord development. Expression of sema1a and the plexin A (plexA receptor are detected in the embryonic ventral nerve cords of Aedes aegypti (dengue vector and Anopheles gambiae (malaria vector, suggesting that Sema1a signaling may regulate mosquito nervous system development. Analysis of sema1a function was investigated through siRNA-mediated knockdown in A. aegypti embryos. Knockdown of sema1a during A. aegypti development results in a number of nerve cord phenotypes, including thinning, breakage, and occasional fusion of the longitudinal connectives, thin or absent commissures, and general distortion of the nerve cord. Although analysis of Drosophila melanogaster sema1a loss-of-function mutants uncovered many similar phenotypes, aspects of the longitudinal phenotypes differed between D. melanogaster and A. aegypti. The results of this investigation suggest that Sema1a is required for development of the insect ventral nerve cord, but that the developmental roles of this guidance molecule have diverged in dipteran insects.

  3. Transgenic expression of the Aedes aegypti CYP9J28 confers pyrethroid resistance in Drosophila melanogaster

    NARCIS (Netherlands)

    Pavlidi, N.; Monastirioti, M.; Daborn, P.; Van Leeuwen, T.; Vontas, J.

    2012-01-01

    The emergence and spread of insecticide resistance in mosquitoes, such as the major vector of dengue and yellow fever Aedes aegypti, is a major public health problem. A number of studies have been conducted to-date aiming to identify specific molecular changes that are associated with the phenotype,

  4. Evaluación de estrategias comunitarias para el control de Aedes aegypti en Cali, Colombia

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    Clara Beatriz Ocampo

    2009-06-01

    Conclusiones. La ausencia de diferencias significativas entre las intervenciones y el bloque control sugiere que las actividades educacionales junto con las visitas periódicas a las casas producen reducciones similares de los estadios inmaduros y adultos de Ae. aegypti.

  5. First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas.

    Directory of Open Access Journals (Sweden)

    André Luis Costa-da-Silva

    2017-06-01

    Full Text Available The worldwide expansion of new emergent arboviruses such as Chikungunya and Zika reinforces the importance in understanding the role of mosquito species in spreading these pathogens in affected regions. This knowledge is essential for developing effective programs based on species specificity to avoid the establishment of endemic transmission cycles sustained by the identified local vectors. Although the first autochthonous transmission of Chikungunya virus was described in 2014 in the north of Brazil, the main outbreaks were reported in 2015 and 2016 in the northeast of Brazil.During 5 days of February 2016, we collected mosquitoes in homes of 6 neighborhoods of Aracaju city, the capital of Sergipe state. Four mosquito species were identified but Culex quinquefasciatus and Aedes aegypti were the most abundant. Field-caught mosquitoes were tested for Chikungunya (CHIKV, Zika (ZIKV and Dengue viruses (DENV by qRT-PCR and one CHIKV-infected Ae. aegypti female was detected. The complete sequence of CHIKV genome was obtained from this sample and phylogenetic analysis revealed that this isolate belongs to the East-Central-South-African (ECSA genotype.Our study describes the first identification of a naturally CHIKV-infected Ae. aegypti in Brazil and the first report of a CHIKV from ECSA genotype identified in this species in the Americas. These findings support the notion of Ae. aegypti being a vector involved in CHIKV outbreaks in northeast of Brazil.

  6. Synthesis and Mosquitocidal Activity of a Series of Hydrazone Derivatives against Aedes aegypti

    Science.gov (United States)

    Background: Aedes aegypti is an important mosquito vector for the transmission of several infectious diseases. Current insecticides play a vital role in controlling mosquitoes; however, the frequent use of insecticides has led to the development of insecticide resistance. In order to control mosquit...

  7. Spatial repellency screening in a high-throughput apparatus with Aedes aegypti and Anopheles gambiae

    Science.gov (United States)

    Spatial repellents are essential for personal protection against mosquitoes, such as Aedes aegypti and Anopheles gambiae, to reduce annoyance biting and transmission of mosquito-borne diseases. The number of safe and effective repellents, including DEET, picaridin, and IR3535, is limited and contin...

  8. Identification of insecticidal principals from cucumber seed oil against the yellow fever mosquito, Aedes aegypti

    Science.gov (United States)

    The yellow fever mosquito, Aedes aegypti, is one of the most medically important mosquito species due to its ability to spread viruses of yellow fever, dengue fever and Zika in humans. In this study, the insecticidal activity of seventeen plant essential oils were evaluated to toxicity by topical a...

  9. Laboratory Evaluation of a Novel Lethal Ovitrap for Control of Aedes aegypti

    Science.gov (United States)

    Aedes aegypti and Aedes albopictus are known to flourish in a variety of natural and residential habitats and are competent vectors of at least 22 different arboviruses including dengue, chikungunya, and zika. Their global distribution, anthropophilic nature, and vector competency make them species ...

  10. On the analysis of parasite effect for Aedes aegypti and Aedes albopictus population

    Science.gov (United States)

    Kallista, Meta; Aldila, Dipo; Nuraini, Nuning; Soewono, Edy

    2014-03-01

    It has been reported in some countries that the population of Aedes aegypti has been significantly reduced by the invasion of Aedes albopictus. There has been a hypothesis explaining this phenomenon of which investigated the influence of parasites pathogenesis to the competition between these two mosquito species in the fields. Ascogregarina taiwanensis and Ascogregarina culicis are known as parasites that infect Aedes albopictus and Aedes aegypti, respectively. Several studies have concluded that Ascogregarina taiwanensis caused high fatality for Aedes aegypti larvae, but Ascogregarina culicis was not pathogenic to Aedes albopictus larvae. Therefore, Ascogregarina taiwanensis may contribute to reduce the number of populations Aedes aegypti in the fields. Inspired by these facts, a mathematical model depicting interaction between parasites and mosquitoes is constructed in this paper. In this model are included six dynamic mosquito compartments, i.e. egg, larvae, infected larvae, adult, infected adult and one dynamic compartment for parasite. Derivation of the existence criteria and the stability analysis of parasite-free equilibrium as well as the basic offspring for the model are presented. Numerical simulations for sensitivity analysis indicating the invasive species for variation parameters are shown.

  11. Wingbeat frequency-sweep and visual stimuli for trapping male Aedes aegypti (Diptera: Culicidae)

    Science.gov (United States)

    Combinations of female wingbeat acoustic cues and visual cues were evaluated to determine their potential for use in male Aedes aegypti (L.) traps in peridomestic environments. A modified Centers for Disease control (CDC) light trap using a 350-500 Hz frequency-sweep broadcast from a speaker as an a...

  12. Vertical Transmission of Zika Virus by Aedes aegypti and Ae. albopictus Mosquitoes.

    Science.gov (United States)

    Ciota, Alexander T; Bialosuknia, Sean M; Ehrbar, Dylan J; Kramer, Laura D

    2017-05-01

    To determine the potential role of vertical transmission in Zika virus expansion, we evaluated larval pools of perorally infected Aedes aegypti and Ae. albopictus adult female mosquitoes; ≈1/84 larvae tested were Zika virus-positive; and rates varied among mosquito populations. Thus, vertical transmission may play a role in Zika virus spread and maintenance.

  13. Ovicidal activity of Ageratina adenophora (Family: Asteraceae) against dengue vector, Aedes aegypti (Diptera: Culicidae)

    Science.gov (United States)

    To determine the ovicidal efficacy of different solvent leaf extracts of Ageratina adenophora against dengue vector Aedes aegypti . Methods: The ovicidal efficacy of the crude leaf extracts of A. adenophora with five different solvents (hexane, benzene, chloroform, ethyl acetate, methanol) and was ...

  14. Resistance to deltamethrine in two populations of Aedes aegypti (Diptera, Culicidae from Peru

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    Julio Chávez

    2013-05-01

    Full Text Available The objective of the present work was to determine the resistance levels to deltamethrine in two populations of Aedes aegypti from Peru. Bioassays in adults were carried out following the methodology of the World Health Organization. We met resistance in the Sullana population with 70% of mortality and susceptibility in the population The Future El Porvenir with 99% of mortality.

  15. Genetics and morphology of Aedes aegypti (Diptera: Culicidae) in septic tanks in Puerto Rico.

    Science.gov (United States)

    Somers, Gerard; Brown, Julia E; Barrera, Roberto; Powell, Jeffrey R

    2011-11-01

    Dengue viruses, primarily transmitted by the mosquito Aedes aegypti (L.), affect an estimated 50-100 million people yearly. Traditional approaches to control mosquito population numbers, such as the use of pesticides, have had only limited success. Atypical mosquito behavior may be one reason why current vector control efforts have been less efficacious than expected. In Puerto Rico, for example, adult Ae. aegypti have been observed emerging from septic tanks. Interestingly, adults emerging from septic tanks are larger on average than adults collected from surface containers. To determine whether adults colonizing septic tanks constitute a separate Ae. aegypti population, we used 12 previously validated microsatellite loci to examine adult mosquitoes collected from both septic tanks and surface containers, but found no evidence to suggest genetic differentiation. Size differences between septic tank and surface mosquitoes were reduced when nutrient levels were held constant across experimental groups. Despite the absence of evidence suggesting a genetic difference between experimental groups in this study, Ae. aegypti emerging from septic tanks may still represent a more dangerous phenotype and should be given special consideration when developing vector control programs and designing public health interventions in the future.

  16. A new tent trap for monitoring the daily activity of Aedes aegypti and Aedes albopictus.

    Science.gov (United States)

    Casas Martínez, Mauricio; Orozco Bonilla, Arnoldo; Muñoz Reyes, Miguel; Ulloa García, Armando; Bond, J Guillermo; Valle Mora, Javier; Weber, Manuel; Rojas, Julio C

    2013-12-01

    In this study, we designed a new tent trap; the BioDiVector (BDV) tent trap, consisting of two rectangular tents that use human bait without endangering the technical personnel. The daily activity pattern of Aedes aegypti and Aedes albopictus in intra, peri, and extradomiciliary sites was studied in an endemic area of dengue in southern Mexico by using the BDV tent trap. Totals of 3,128 individuals of Ae. aegypti and 833 Ae. albopictus were captured. More Ae. aegypti males than females were caught, while the opposite was true with Ae. albopictus. The activity of both mosquito species was affected by the interaction between the collection site and time of day. In general, more individuals of both mosquito species were captured at the extradomicillary sites than at the peri and intradomicillary sites. Mosquitoes showed two peaks of activity, one in the morning and the other in the afternoon, but in general this only occurred at the extradomicillary sites, whereas no peak of activity was observed at the intra and peridomicillary sites. Overall, Ae. aegypti had a higher indirect biting rate than Ae. albopictus. Finally, due to its efficiency, simplicity, and low cost, we suggest the use of this innovative tool for entomological surveillance, bionomics and vector incrimination studies in geographical areas where dengue and other arboviruses are present. © 2013 The Society for Vector Ecology.

  17. TALEN-Based Gene Disruption in the Dengue Vector Aedes aegypti

    Science.gov (United States)

    Aryan, Azadeh; Anderson, Michelle A. E.; Myles, Kevin M.; Adelman, Zach N.

    2013-01-01

    In addition to its role as the primary vector for dengue viruses, Aedes aegypti has a long history as a genetic model organism for other bloodfeeding mosquitoes, due to its ease of colonization, maintenance and reproductive productivity. Though its genome has been sequenced, functional characterization of many Ae. aegypti genes, pathways and behaviors has been slow. TALE nucleases (TALENs) have been used with great success in a number of organisms to generate site-specific DNA lesions. We evaluated the ability of a TALEN pair to target the Ae. aegypti kmo gene, whose protein product is essential in the production of eye pigmentation. Following injection into pre-blastoderm embryos, 20–40% of fertile survivors produced kmo alleles that failed to complement an existing khw mutation. Most of these individuals produced more than 20% white-eyed progeny, with some producing up to 75%. Mutant alleles were associated with lesions of 1–7 bp specifically at the selected target site. White-eyed individuals could also be recovered following a blind intercross of G1 progeny, yielding several new white-eyed strains in the genetic background of the sequenced Liverpool strain. We conclude that TALENs are highly active in the Ae. aegypti germline, and have the potential to transform how reverse genetic experiments are performed in this important disease vector. PMID:23555893

  18. TALEN-based gene disruption in the dengue vector Aedes aegypti.

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    Azadeh Aryan

    Full Text Available In addition to its role as the primary vector for dengue viruses, Aedes aegypti has a long history as a genetic model organism for other bloodfeeding mosquitoes, due to its ease of colonization, maintenance and reproductive productivity. Though its genome has been sequenced, functional characterization of many Ae. aegypti genes, pathways and behaviors has been slow. TALE nucleases (TALENs have been used with great success in a number of organisms to generate site-specific DNA lesions. We evaluated the ability of a TALEN pair to target the Ae. aegypti kmo gene, whose protein product is essential in the production of eye pigmentation. Following injection into pre-blastoderm embryos, 20-40% of fertile survivors produced kmo alleles that failed to complement an existing kh(w mutation. Most of these individuals produced more than 20% white-eyed progeny, with some producing up to 75%. Mutant alleles were associated with lesions of 1-7 bp specifically at the selected target site. White-eyed individuals could also be recovered following a blind intercross of G1 progeny, yielding several new white-eyed strains in the genetic background of the sequenced Liverpool strain. We conclude that TALENs are highly active in the Ae. aegypti germline, and have the potential to transform how reverse genetic experiments are performed in this important disease vector.

  19. The Dengue Virus Mosquito Vector Aedes aegypti at High Elevation in México

    Science.gov (United States)

    Lozano-Fuentes, Saul; Hayden, Mary H.; Welsh-Rodriguez, Carlos; Ochoa-Martinez, Carolina; Tapia-Santos, Berenice; Kobylinski, Kevin C.; Uejio, Christopher K.; Zielinski-Gutierrez, Emily; Monache, Luca Delle; Monaghan, Andrew J.; Steinhoff, Daniel F.; Eisen, Lars

    2012-01-01

    México has cities (e.g., México City and Puebla City) located at elevations > 2,000 m and above the elevation ceiling below which local climates allow the dengue virus mosquito vector Aedes aegypti to proliferate. Climate warming could raise this ceiling and place high-elevation cities at risk for dengue virus transmission. To assess the elevation ceiling for Ae. aegypti and determine the potential for using weather/climate parameters to predict mosquito abundance, we surveyed 12 communities along an elevation/climate gradient from Veracruz City (sea level) to Puebla City (∼2,100 m). Ae. aegypti was commonly encountered up to 1,700 m and present but rare from 1,700 to 2,130 m. This finding extends the known elevation range in México by > 300 m. Mosquito abundance was correlated with weather parameters, including temperature indices. Potential larval development sites were abundant in Puebla City and other high-elevation communities, suggesting that Ae. aegypti could proliferate should the climate become warmer. PMID:22987656

  20. Pyrethroid Susceptibility Has Been Maintained in the Dengue Vector, Aedes aegypti (Diptera: Culicidae), in Queensland, Australia.

    Science.gov (United States)

    Endersby-Harshman, Nancy M; Wuliandari, Juli Rochmijati; Harshman, Lawrence G; Frohn, Verena; Johnson, Brian J; Ritchie, Scott A; Hoffmann, Ary A

    2017-11-07

    Although pesticide resistance is common in insect vectors of human diseases, the evolution of resistance might be delayed if management practices are adopted that limit selection of resistance alleles. Outbreaks of dengue fever have occurred in Queensland, Australia, since the late 1800s, leading to ongoing attempts to control the mosquito vector, Aedes aegypti (L.). Since the 1990s, pyrethroid insecticides have been used for this purpose, but have been applied in a strategic manner with a variety of delivery methods including indoor residual spraying, lethal ovitraps, and use of insect growth regulators as larvicides. Separate selection experiments on mosquitoes from Queensland using Type I and Type II pyrethroids did not produce resistant lines of Ae. aegypti, and bioassays of field material from Queensland showed only weak tolerance in comparison with a susceptible line. There was no evidence of knockdown resistance (kdr) mutations in Ae. aegypti from Queensland, in stark contrast to the situation in nearby southeast Asia. We suspect that careful management of pyrethroid insecticide use combined with surveillance and interception of exotic incursions has helped to maintain pyrethroid (and particularly kdr-based) susceptibility in Ae. aegypti in Australia. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  1. Complement-related proteins control the flavivirus infection of Aedes aegypti by inducing antimicrobial peptides.

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    Xiaoping Xiao

    2014-04-01

    Full Text Available The complement system functions during the early phase of infection and directly mediates pathogen elimination. The recent identification of complement-like factors in arthropods indicates that this system shares common ancestry in vertebrates and invertebrates as an immune defense mechanism. Thioester (TE-containing proteins (TEPs, which show high similarity to mammalian complement C3, are thought to play a key role in innate immunity in arthropods. Herein, we report that a viral recognition cascade composed of two complement-related proteins limits the flaviviral infection of Aedes aegypti. An A. aegypti macroglobulin complement-related factor (AaMCR, belonging to the insect TEP family, is a crucial effector in opposing the flaviviral infection of A. aegypti. However, AaMCR does not directly interact with DENV, and its antiviral effect requires an A. aegypti homologue of scavenger receptor-C (AaSR-C, which interacts with DENV and AaMCR simultaneously in vitro and in vivo. Furthermore, recognition of DENV by the AaSR-C/AaMCR axis regulates the expression of antimicrobial peptides (AMPs, which exerts potent anti-DENV activity. Our results both demonstrate the existence of a viral recognition pathway that controls the flaviviral infection by inducing AMPs and offer insights into a previously unappreciated antiviral function of the complement-like system in arthropods.

  2. Infection of adult Aedes aegypti and Ae. albopictus mosquitoes with the entomopathogenic fungus Metarhizium anisopliae

    NARCIS (Netherlands)

    Scholte, E.J.; Takken, W.; Knols, B.G.J.

    2007-01-01

    This study describes a laboratory investigation on the use of the insect-pathogenic fungus Metarhizium anisopliae against adult Aedes aegypti and Ae. albopictus mosquitoes. At a dosage of 1.6 × 1010 conidia/m2, applied on material that served as a mosquito resting site, an average of 87.1 ± 2.65% of

  3. Insecticide resistance is mediated by multiple mechanisms in recently introduced Aedes aegypti from Madeira Island (Portugal).

    Science.gov (United States)

    Seixas, Gonçalo; Grigoraki, Linda; Weetman, David; Vicente, José Luís; Silva, Ana Clara; Pinto, João; Vontas, John; Sousa, Carla Alexandra

    2017-07-01

    Aedes aegypti is a major mosquito vector of arboviruses, including dengue, chikungunya and Zika. In 2005, Ae. aegypti was identified for the first time in Madeira Island. Despite an initial insecticide-based vector control program, the species expanded throughout the Southern coast of the island, suggesting the presence of insecticide resistance. Here, we characterized the insecticide resistance status and the underlying mechanisms of two populations of Ae. aegypti from Madeira Island, Funchal and Paúl do Mar. WHO susceptibility bioassays indicated resistance to cyfluthrin, permethrin, fenitrothion and bendiocarb. Use of synergists significantly increased mortality rates, and biochemical assays indicated elevated activities of detoxification enzymes, suggesting the importance of metabolic resistance. Microarray-based transcriptome analysis detected significant upregulation in both populations of nine cytochrome P450 oxidase genes (including four known pyrethroid metabolizing enzymes), the organophosphate metabolizer CCEae3a, Glutathione-S-transferases, and multiple putative cuticle proteins. Genotyping of knockdown resistance loci linked to pyrethroid resistance revealed fixation of the 1534C mutation, and presence with moderate frequencies of the V1016I mutation in each population. Significant resistance to three major insecticide classes (pyrethroid, carbamate and organophosphate) is present in Ae. aegypti from Madeira Island, and appears to be mediated by multiple mechanisms. Implementation of appropriate resistance management strategies including rotation of insecticides with alternative modes of action, and methods other than chemical-based vector control are strongly advised to delay or reverse the spread of resistance and achieve efficient control.

  4. Wolbachia infection does not alter attraction of the mosquito Aedes (Stegomyia) aegypti to human odours

    NARCIS (Netherlands)

    Turley, A.P.; Smallegange, R.C.; Takken, W.; Zalucki, M.P.; O'Neill, S.L.; McGraw, E.A.

    2014-01-01

    The insect endosymbiont Wolbachia pipientis (Rickettsiales: Rickettsiaceae) is undergoing field trials around the world to determine if it can reduce transmission of dengue virus from the mosquito Stegomyia aegypti to humans. Two different Wolbachia strains have been released to date. The primary

  5. [Behavior of immatures Aedes aegypti in the coast State of São Paulo, Brazil].

    Science.gov (United States)

    Glasser, Carmen Moreno; Arduino, Marylene de Brito; Barbosa, Gerson Laurindo; Ciaravolo, Ricardo Mario de Carvalho; Domingos, Maria de Fátima; Oliveira, Cleide Dantas; Pereira, Marisa; Silva, Marcos; Trevisan, Alexandra Myuki Yoshioka

    2011-01-01

    In a region of high dengue incidence, on the coast of the State of São Paulo, 9 areas were selected to evaluate the behavior of immature Aedes aegypti. The 9 areas were grouped into 4 strata according to soil use and occupation. Larvae and pupas were collected in a sample of approximately 500 buildings in each area. Although tires and canvas presented the highest positive rates for Aedes aegypti, drains and other containers fixed to the buildings were highly predominant among positive containers; 32 to 76% of the positive containers in the 4 study strata. Public areas of apartment buildings and large non-residential premises presented the highest positive rates for Aedes Aegypti, while apartments presented the lowest. Infestation levels were greater in residential areas with predominance of apartment buildings, where 76% of the breeding sites detected were containers fixed to the buildings. This knowledge is an important tool in the control strategy, since it reinforces the need for special attention regarding certain types of buildings and the adjustment of technical norms for pluvial water drains and improvement of building maintenance. Moreover, systematic observations are required to follow-up the occupancy dynamic of different buildings and containers by Aedes aegypti and the incorporation of this knowledge in the control of vectors in the region.

  6. Behavioral Response of Aedes aegypti Mosquito towards Essential Oils Using Olfactometer

    Science.gov (United States)

    Uniyal, Ashish; Tikar, Sachin N; Mendki, Murlidhar J; Singh, Ram; Shukla, Shakti V; Agrawal, Om P; Veer, Vijay; Sukumaran, Devanathan

    2016-01-01

    Background: Aedes aegypti mosquito is responsible for transmitting human diseases like dengue and chikungunya. Personal or space protection with insect repellents is a practical approach to reducing human mosquito contact, thereby minimizing disease transmission. Essential oils are natural volatile substances from plants used as protective measure against blood-sucking mosquitoes. Methods: Twenty-three essential oils were evaluated for their repellent effect against Ae. aegypti female mosquito in laboratory conditions using Y-tube olfactometer. Results: The essential oils exhibited varying degree of repellency. Litsea oil showed 50.31%, 60.2 %, and 77.26% effective mean repellency at 1 ppm, 10 ppm and 100 ppm respectively, while DEET exhibited 59.63%, 68.63%, 85.48% and DEPA showed 57.97%, 65.43%, and 80.62% repellency at respective above concentrations. Statistical analysis revealed that among the tested essential oils, litsea oil had effective repellency in comparison with DEET and DEPA against Ae. aegypti mosquito at all concentration. Essential oils, DEET and DEPA showed significant repellence against Ae. aegypti (Poil exhibited effective percentage repellency similar to DEET and DEPA. The essential oils are natural plant products that may be useful for developing safer and newer herbal based effective mosquito repellents. PMID:27308295

  7. EKSISTENSI DAN SEBARAN NYAMUK AEDES AEGYPTI DAN AEDES ALBOPICTUS DI KAMPUS UNIVERSITAS HASANUDDIN MAKASSAR

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    Yosefina Dota T

    2015-03-01

    Full Text Available Penelitian yang bertujuan untuk mengetahui eksistensi dan sebaran nyamuk Aedes aegypti dan Aedes albopictus telah dilakukan di Kampus Universitas Hasanuddin, Kec. Tamalanrea, Makassar. Penelitian bersifat eksploratif dengan melakukan sampling terhadap lima lokasi yaitu : a Fak. Peternakan (Utara, b Fak. Hukum (Timur, c Pusat Kegiatan Penelitian/PKP (Selatan, d Workshop/Dekat Pondokan mahasiswa (Barat dan e Fak. MIPA (Tengah. Sampling nyamuk menggunakan metode ovitrap (menggunakan attraktan Eluisine Indica L. dan survei terhadap berbagai tempat penampungan air. Sampel telur dan larva nyamuk yang diperoleh disimpan dalam microtube berisi alkohol 70% kemudian diidentifikasi berdasarkan Rueda (2004. Hasil penelitian menunjukkan bahwa Nyamuk Ae. aegypti dan Ae. albopictus ditemukan hidup dan berkembang biak di kampus Universitas Hasanuddin, Makassar. Eksistensi dan sebaran kedua jenis nyamuk tersebut dipengaruhi oleh faktor adanya manusia/masyarakat kampus yang beraktivitas baik di dalam ruangan (indoor maupun di luar ruangan (outdoor, adanya berbagai tempat penampungan air baik buatan (bak mandi, ember maupun barang bekas (botol/kaleng bekas, tempurung kelapa, vegetasi/tanaman dan berbagai macam hewan yang berada di sekitaran kampus. Hasil penelitian dalam ruangan (indoor menunjukkan bahwa nyamuk Ae. aegypti lebih banyak ditemukan hidup di dalam ruangan gedung PKP sedangkan Ae.albopictus lebih banyak di Fak. Hukum. Hasil penelitian di luar ruangan (outdoor menunjukkan bahwa nyamuk Ae. aegypti lebih banyak ditemukan hidup di area Workshop sedangkan Ae.albopictus lebih banyak di area PKP.

  8. Efikasi Ekstrak Daun dan Bunga Kecombrang (Etlingera elatior terhadap Larva Aedes aegypti

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    Meiske Elisabeth Koraag

    2016-12-01

    Full Text Available Abstract. Three widely known dengue vector control programs in Indonesia are chemical, biological, and environmental modification control where chemical control with organophosphate insecticide (malathion and temephos is the most common. The long term use of chemical insecticide will result in the vector beingtolerant and eventually resistant to insecticide. One of the alternative solutions is to use biological larvacide from the plant. The objective of this study was to determine the lethal concentration of the extract of Kecombrang (Etlingera elatior leaves and flowers against Aedes aegypti larvae. This was an experimentalstudy where the sample size was determined by using the Federer formula. The study used six different concentrations and four repetitions. Two controls group, Bacillus thuringiensis and water used as positive and negative control. The results showed that the LC50 and LC90 of Kecombrang leave extract were 1.20% and 2.05% respectively whereas for Kecombrang flowers extract were 0,05% and 0.09% respectively. Extract of Kecombrang leaves and flowers is effective to kill Ae. aegypti larvae where the flowers extract is more effective than the leaves extract in killing Ae. aegypti larvae.Keywords: dengue, Ae. aegypti, larvae, Etlingera elatiorAbstrak. Pengendalian vektor penular demam berdarah dengue (DBD yang selama ini dikenal yaitu pengendalian secara kimia, biologi dan modifikasi lingkungan. Pengendalian vektor DBD di Indonesia masih banyak dilakukan dengan menggunakan insektisida dari golongan organofosfat (malation dan temefos. Penggunaan insektisida kimia dalam jangka waktu lama akan memberi efek menekan dan menyeleksi serangga vektor sasaran untuk menjadi toleran sampai resisten. Salah satu solusi alternatif yaitu menggunakan larvasida yang berasal dari tanaman. Penelitian ini bertujuan untuk menentukan daya bunuh ekstrak daun dan bunga kecombrang (Etlingera elatior terhadap larva nyamuk Ae. aegypti. Jenis penelitian yang

  9. Oviposition-stimulant and ovicidal activities of Moringa oleifera lectin on Aedes aegypti.

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    Nataly Diniz de Lima Santos

    Full Text Available Natural insecticides against the vector mosquito Aedes aegypti have been the object of research due to their high level of eco-safety. The water-soluble Moringa oleifera lectin (WSMoL is a larvicidal agent against A. aegypti. This work reports the effects of WSMoL on oviposition and egg hatching of A. aegypti.WSMoL crude preparations (seed extract and 0-60 protein fraction, at 0.1 mg/mL protein concentration, did not affect oviposition, while A. aegypti gravid females laid their eggs preferentially (73% in vessels containing isolated WSMoL (0.1 mg/mL, compared with vessels containing only distilled water (control. Volatile compounds were not detected in WSMoL preparation. The hatchability of fresh eggs deposited in the solutions in the oviposition assay was evaluated. The numbers of hatched larvae in seed extract, 0-60 protein fraction and WSMoL were 45 ± 8.7 %, 20 ± 11 % and 55 ± 7.5 %, respectively, significantly (p<0.05 lower than in controls containing only distilled water (75-95%. Embryos were visualized inside fresh control eggs, but not within eggs that were laid and maintained in WSMoL solution. Ovicidal activity was also assessed using stored A. aegypti eggs. The protein concentrations able to reduce the hatching rate by 50% (EC50 were 0.32, 0.16 and 0.1 mg/mL for seed extract, 0-60 protein fraction and WSMoL, respectively. The absence of hatching of stored eggs treated with WSMoL at 0.3 mg/mL (EC99 after transfer to medium without lectin indicates that embryos within the eggs were killed by WSMoL. The reduction in hatching rate of A. aegypti was not linked to decrease in bacteria population.WSMoL acted both as a chemical stimulant cue for ovipositing females and ovicidal agent at a given concentration. The oviposition-stimulant and ovicidal activities, combined with the previously reported larvicidal activity, make WSMoL a very interesting candidate in integrated A. aegypti control.

  10. The Aquaporin gene family of the yellow fever mosquito, Aedes aegypti.

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    Lisa L Drake

    2010-12-01

    Full Text Available The mosquito, Aedes aegypti, is the principal vector of the Dengue and yellow fever viruses. During feeding, an adult female can take up more than its own body weight in vertebrate blood. After a blood meal females excrete large amounts of urine through their excretion system, the Malpighian tubules (MT. Diuresis starts within seconds after the mosquito starts feeding. Aquaporins (AQPs are a family of membrane transporters that regulate the flow of water, glycerol and other small molecules across cellular membranes in both prokaryotic and eukaryotic cells. Our aim was to identify aquaporins that function as water channels, mediating transcellular water transport in MTs of adult female Ae. aegypti.Using a bioinformatics approach we screened genome databases and identified six putative AQPs in the genome of Ae. aegypti. Phylogenetic analysis showed that five of the six Ae. aegypti AQPs have high similarity to classical water-transporting AQPs of vertebrates. Using microarray, reverse transcription and real time PCR analysis we found that all six AQPs are expressed in distinct patterns in mosquito tissues/body parts. AaAQP1, 4, and 5 are strongly expressed in the adult female MT. RNAi-mediated knockdown of the MT-expressed mosquito AQPs resulted in significantly reduced diuresis.Our results support the notion that AQP1, 4, and 5 function as water transporters in the MTs of adult female Ae. aegypti mosquitoes. Our results demonstrate the importance of these AQPs for mosquito diuresis after blood ingestion and highlight their potential as targets for the development of novel vector control strategies.

  11. The Aquaporin gene family of the yellow fever mosquito, Aedes aegypti.

    Science.gov (United States)

    Drake, Lisa L; Boudko, Dmitri Y; Marinotti, Osvaldo; Carpenter, Victoria K; Dawe, Angus L; Hansen, Immo A

    2010-12-29

    The mosquito, Aedes aegypti, is the principal vector of the Dengue and yellow fever viruses. During feeding, an adult female can take up more than its own body weight in vertebrate blood. After a blood meal females excrete large amounts of urine through their excretion system, the Malpighian tubules (MT). Diuresis starts within seconds after the mosquito starts feeding. Aquaporins (AQPs) are a family of membrane transporters that regulate the flow of water, glycerol and other small molecules across cellular membranes in both prokaryotic and eukaryotic cells. Our aim was to identify aquaporins that function as water channels, mediating transcellular water transport in MTs of adult female Ae. aegypti. Using a bioinformatics approach we screened genome databases and identified six putative AQPs in the genome of Ae. aegypti. Phylogenetic analysis showed that five of the six Ae. aegypti AQPs have high similarity to classical water-transporting AQPs of vertebrates. Using microarray, reverse transcription and real time PCR analysis we found that all six AQPs are expressed in distinct patterns in mosquito tissues/body parts. AaAQP1, 4, and 5 are strongly expressed in the adult female MT. RNAi-mediated knockdown of the MT-expressed mosquito AQPs resulted in significantly reduced diuresis. Our results support the notion that AQP1, 4, and 5 function as water transporters in the MTs of adult female Ae. aegypti mosquitoes. Our results demonstrate the importance of these AQPs for mosquito diuresis after blood ingestion and highlight their potential as targets for the development of novel vector control strategies.

  12. Water Level Flux in Household Containers in Vietnam - A Key Determinant of Aedes aegypti Population Dynamics

    Science.gov (United States)

    Jeffery, Jason A. L.; Clements, Archie C. A.; Nguyen, Yen Thi; Nguyen, Le Hoang; Tran, Son Hai; Le, Nghia Trung; Vu, Nam Sinh; Ryan, Peter A.; Kay, Brian H.

    2012-01-01

    We examined changes in the abundance of immature Aedes aegypti at the household and water storage container level during the dry-season (June-July, 2008) in Tri Nguyen village, central Vietnam. We conducted quantitative immature mosquito surveys of 171 containers in the same 41 households, with replacement of samples, every two days during a 29-day period. We developed multi-level mixed effects regression models to investigate container and household variability in pupal abundance. The percentage of houses that were positive for I/II instars, III/IV instars and pupae during any one survey ranged from 19.5–43.9%, 48.8–75.6% and 17.1–53.7%, respectively. The mean numbers of Ae. aegypti pupae per house ranged between 1.9–12.6 over the study period. Estimates of absolute pupal abundance were highly variable over the 29-day period despite relatively stable weather conditions. Most variability in pupal abundance occurred at the container rather than the household level. A key determinant of Ae. aegypti production was the frequent filling of the containers with water, which caused asynchronous hatching of Ae. aegypti eggs and development of cohorts of immatures. We calculated the probability of the water volume of a large container (>500L) increasing or decreasing by ≥20% to be 0.05 and 0.07 per day, respectively, and for small containers (<500L) to be 0.11 and 0.13 per day, respectively. These human water-management behaviors are important determinants of Ae. aegypti production during the dry season. This has implications for choosing a suitable Wolbachia strain for release as it appears that prolonged egg desiccation does not occur in this village. PMID:22911683

  13. Aedes aegypti uses RNA interference in defense against Sindbis virus infection.

    Science.gov (United States)

    Campbell, Corey L; Keene, Kimberly M; Brackney, Douglas E; Olson, Ken E; Blair, Carol D; Wilusz, Jeffrey; Foy, Brian D

    2008-03-17

    RNA interference (RNAi) is an important anti-viral defense mechanism. The Aedes aegypti genome encodes RNAi component orthologs, however, most populations of this mosquito are readily infected by, and subsequently transmit flaviviruses and alphaviruses. The goal of this study was to use Ae. aegypti as a model system to determine how the mosquito's anti-viral RNAi pathway interacts with recombinant Sindbis virus (SINV; family Togaviridae, genus Alphavirus). SINV (TR339-eGFP) (+) strand RNA, infectious virus titers and infection rates transiently increased in mosquitoes following dsRNA injection to cognate Ago2, Dcr2, or TSN mRNAs. Detection of SINV RNA-derived small RNAs at 2 and 7 days post-infection in non-silenced mosquitoes provided important confirmation of RNAi pathway activity. Two different recombinant SINV viruses (MRE16-eGFP and TR339-eGFP) with significant differences in infection kinetics were used to delineate vector/virus interactions in the midgut. We show virus-dependent effects on RNAi component transcript and protein levels during infection. Monitoring midgut Ago2, Dcr2, and TSN transcript levels during infection revealed that only TSN transcripts were significantly increased in midguts over blood-fed controls. Ago2 protein levels were depleted immediately following a non-infectious bloodmeal and varied during SINV infection in a virus-dependent manner. We show that silencing RNAi components in Ae. aegypti results in transient increases in SINV replication. Furthermore, Ae. aegypti RNAi is active during SINV infection as indicated by production of virus-specific siRNAs. Lastly, the RNAi response varies in a virus-dependent manner. These data define important features of RNAi anti-viral defense in Ae. aegypti.

  14. Oral susceptibility of Singapore Aedes (Stegomyia aegypti (Linnaeus to Zika virus.

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    MeiZhi Irene Li

    Full Text Available Zika virus (ZIKV is a little known flavivirus that caused a major outbreak in 2007, in the South-western Pacific Island of Yap. It causes dengue-like syndromes but with milder symptoms. In Africa, where it was first isolated, ZIKV is mainly transmitted by sylvatic Aedes mosquitoes. The virus has also been isolated from Ae. aegypti and it is considered to be the vector involved in the urban transmission of the virus. Transmission of the virus by an African strain of Ae. aegypti has also been demonstrated under laboratory conditions. The aim of the present study is to describe the oral susceptibility of a Singapore strain of Ae. aegypti to ZIKV, under conditions that simulate local climate.To assess the receptivity of Singapore's Ae. aegypti to the virus, we orally exposed a local mosquito strain to a Ugandan strain of ZIKV. Upon exposure, fully engorged mosquitoes were maintained in an environmental chamber set at 29 °C and 70-75% RH. Eight mosquitoes were then sampled daily from day 1 to day 7, and subsequently on days 10 and 14 post exposure (pe. The virus titer of the midgut and salivary glands of each mosquito were determined using a tissue culture infectious dose(50 (TCID(50 assay. High midgut infection and salivary gland dissemination rates were observed. By day 5 after the infectious blood meal, ZIKV was found in the salivary glands of more than half of the mosquitoes tested (62%; and by day 10, all mosquitoes were potentially infective.This study showed that Singapore's urban Ae. aegypti are susceptible and are potentially capable of transmitting ZIKV. The virus could be established in Singapore should it be introduced. Nevertheless, Singapore's current dengue control strategy is applicable to control ZIKV.

  15. Effect of an intervention in storm drains to prevent Aedes aegypti reproduction in Salvador, Brazil.

    Science.gov (United States)

    Souza, Raquel Lima; Mugabe, Vánio André; Paploski, Igor Adolfo Dexheimer; Rodrigues, Moreno S; Moreira, Patrícia Sousa Dos Santos; Nascimento, Leile Camila Jacob; Roundy, Christopher Michael; Weaver, Scott C; Reis, Mitermayer Galvão; Kitron, Uriel; Ribeiro, Guilherme Sousa

    2017-07-11

    Aedes aegypti, the principal vector for dengue, chikungunya and Zika viruses, is a synanthropic species that uses stagnant water to complete its reproductive cycle. In urban settings, rainfall water draining structures, such as storm drains, may retain water and serve as a larval development site for Aedes spp. reproduction. Herein, we describe the effect of a community-based intervention on preventing standing water accumulation in storm drains and their consequent infestation by adult and immature Ae. aegypti and other mosquitoes. Between April and May of 2016, local residents association of Salvador, Brazil, after being informed of water accumulation and Ae. aegypti infestation in the storm drains in their area, performed an intervention on 52 storm drains. The intervention consisted of placing concrete at the bottom of the storm drains to elevate their base to the level of the outflow tube, avoiding water accumulation, and placement of a metal mesh covering the outflow tube to avoid its clogging with debris. To determine the impact of the intervention, we compared the frequency at which the 52 storm drains contained water, as well as adult and immature mosquitoes using data from two surveys performed before and two surveys performed after the intervention. During the pre-intervention period, water accumulated in 48 (92.3%) of the storm drains, and immature Ae. aegypti were found in 11 (21.2%) and adults in 10 (19.2%). After the intervention, water accumulated in 5 (9.6%) of the storm drains (P Aedes mosquitoes (mainly Culex spp.) in the storm drains also decreased after the intervention. This study exemplifies how a simple intervention targeting storm drains can result in a major reduction of water retention, and, consequently, impact Ae. aegypti larval populations. Larger and multi-center evaluations are needed to confirm the potential of citywide structural modifications of storm drains to reduce Aedes spp. infestation level.

  16. Aedes aegypti Global Suitability Maps Using a Water Container Energy Balance Model for Dengue Risk Applications

    Science.gov (United States)

    Steinhoff, D.

    2015-12-01

    Dengue infections are estimated to total nearly 400 million per year worldwide, with both the geographic range and the magnitude of infections having increased in the past 50 years. The primary dengue vector mosquito Aedes aegypti is closely associated with humans. It lives exclusively in urban and semi-urban areas, preferentially bites humans, and spends its developmental stages in artificial water containers. Climate regulates the development of Ae. aegypti immature mosquitoes in artificial containers. Potential containers for Ae. aegypti immature development include, but are not limited to, small sundry items (e.g., bottles, cans, plastic containers), buckets, tires, barrels, tanks, and cisterns. Successful development of immature mosquitoes from eggs to larvae, pupae, and eventually adults is largely dependent on the availability of water and the thermal properties of the water in the containers. Recent work has shown that physics-based approaches toward modeling container water properties are promising for resolving the complexities of container water dynamics and the effects on immature mosquito development. An energy balance container model developed by the author, termed the Water Height And Temperature in Container Habitats Energy Model (WHATCH'EM), solves for water temperature and height for user-specified containers with readily available weather data. Here we use WHATCH'EM with NASA Earth Science products used as input to construct global suitability maps based on established water temperature ranges for immature Ae. aegypti mosquitoes. A proxy for dengue risk is provided from habitat suitability, but also population estimates, as Ae. aegypti is closely associated with human activity. NASA gridded Global Population of the World data is used to mask out rural areas with low dengue risk. Suitability maps are illustrated for a variety of containers (size, material, color) and shading scenarios.

  17. Potential for dengue in South Africa: mosquito ecology with particular reference to Aedes aegypti.

    Science.gov (United States)

    Kemp, A; Jupp, P G

    1991-12-01

    Observations on prevalence, geographical distribution, utilization of artificial larval habitats and anthropophilism were made on diurnal mosquitoes at selected localities along the coast of Natal and inland in the Transvaal to identify potential vectors of dengue in South Africa. Larval collections made in artificial containers on the ground, the exposure of bamboo pots as ovitraps in trees and collection of mosquitoes biting man showed the following species as the most likely candidates for vectors: Aedes aegypti, Ae. demeilloni, Ae. simpsoni, Ae. strelitziae, Ae. furcifer, Ae. cordellieri and Eretmapodites quinquevittatus. The bamboo pots showed that Ae. aegypti and Ae. simpsoni were the most widespread species, occurring at 11 of 12 localities. Aedes aegypti was the most prevalent species with mean pot index of 60.3 +/- 9.8% (SE) and abundance index of 0.43 +/- 0.15 (SE). Aedes aegypti was frequently present as larvae in artificial containers at indices of 11-83% (mean 56.8 +/- 5.6%, SE) and was the most anthropophilic species with average biting rates of 10-29 per man-hour at 7 localities. Although Ae. aegypti was abundant in the pots at Ndumu (northern Natal) and at Skukuza (eastern Transvaal), the local populations were poorly anthropophilic at these localities. At some localities, populations of Ae. demeilloni, Ae. simpsoni and Ae. strelitziae had average biting rates of 5.4-9.6 per man-hour. Aedes furcifer was collected for the first time at Durban, extending its distribution southward to latitude 29 degrees 53' S.

  18. Spatial patterns of high Aedes aegypti oviposition activity in northwestern Argentina.

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    Elizabet Lilia Estallo

    Full Text Available BACKGROUND: In Argentina, dengue has affected mainly the Northern provinces, including Salta. The objective of this study was to analyze the spatial patterns of high Aedes aegypti oviposition activity in San Ramón de la Nueva Orán, northwestern Argentina. The location of clusters as hot spot areas should help control programs to identify priority areas and allocate their resources more effectively. METHODOLOGY: Oviposition activity was detected in Orán City (Salta province using ovitraps, weekly replaced (October 2005-2007. Spatial autocorrelation was measured with Moran's Index and depicted through cluster maps to identify hot spots. Total egg numbers were spatially interpolated and a classified map with Ae. aegypti high oviposition activity areas was performed. Potential breeding and resting (PBR sites were geo-referenced. A logistic regression analysis of interpolated egg numbers and PBR location was performed to generate a predictive mapping of mosquito oviposition activity. PRINCIPAL FINDINGS: Both cluster maps and predictive map were consistent, identifying in central and southern areas of the city high Ae. aegypti oviposition activity. A logistic regression model was successfully developed to predict Ae. aegypti oviposition activity based on distance to PBR sites, with tire dumps having the strongest association with mosquito oviposition activity. A predictive map reflecting probability of oviposition activity was produced. The predictive map delimitated an area of maximum probability of Ae. aegypti oviposition activity in the south of Orán city where tire dumps predominate. The overall fit of the model was acceptable (ROC=0.77, obtaining 99% of sensitivity and 75.29% of specificity. CONCLUSIONS: Distance to tire dumps is inversely associated with high mosquito activity, allowing us to identify hot spots. These methodologies are useful for prevention, surveillance, and control of tropical vector borne diseases and might assist

  19. Raw sewage as breeding site to Aedes (Stegomyia) aegypti (Diptera, culicidae).

    Science.gov (United States)

    Chitolina, R F; Anjos, F A; Lima, T S; Castro, E A; Costa-Ribeiro, M C V

    2016-12-01

    The selection of oviposition sites by females of Aedes (Stegomyia) aegypti is a key factor for the larval survival and egg dispersion and has a direct influence in vector control programs. In this study, we evaluated the aspects of reproductive physiology of Ae. aegypti mosquitoes tested in the presence of raw sewage. Ae. aegypti females were used in oviposition bioassays according to two methodologies: (i) choice assay, in which three oviposition substrates were offered in the same cage: treatment (raw sewage), positive control (distilled water) and negative control (1% sodium hypochlorite) and; (ii) no choice assay, in which only one substrate was available. The physicochemical and microbiological analysis of the raw sewage used in this study indicated virtually no levels of chlorine, low levels of dissolved oxygen and high levels of nitrogenous compounds as well as the presence of Escherichia coli and total fecal coliforms. After 72h of oviposition, the eggs were counted and there was no statistically significant difference (p>0.05) in the oviposition rate between raw sewage and positive control in both methodologies. In addition, females were dissected to evaluate egg-retention and also there were no appreciable differences in egg retention even when raw sewage was the only substrate offered. The data also showed that egg hatching and larvae development occurred normally in the raw sewage. Therefore, the present study suggests that Ae. aegypti can adapt to new sites and lay eggs in polluted water, such as the raw sewage. These findings are of particular importance for the control and surveillance programs against Ae. aegypti in countries where the conditions of poor infrastructure and lack of basic sanitation are still an issue. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. O REALISMO MÁGICO E O ESPAÇO COMO REFÚGIO DA ALTERIDADE EM “A ILHA DOS GATOS PINGADOS” DE JOSÉ J. VEIGA

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    Fabianna Simão Bellizzi Carneiro

    2013-06-01

    Full Text Available This work will review the construction of the relation between the ideological discourse used towards minority groups and the space reserved to them in contemporary narrative, based on the short story “A ilha dos gatos pingados “ (1986, by the writer José J. Veiga. The choice for this theme is justified by the fact that contemporary man, inserted in the context of significant transformations in technological, social, cultural and economical areas, has in the figure of the other an active agent in this process, ie, the other can no longer be seen as disruptor or someone who unsettles the order of things, but as someone who is part of this dynamic, pointing to the rise of a hybrid culture. Along this way, it is important to observe how Literature is portraying this character. Hence it is intender to analyze the constitutive process of the relation between space and otherness in fantastic narrative, represented in this work by the romance mode of Magic Realism. We stress thatit is an analytical work which is not conclusive, so the search is based on bibliographical sources that will be properly referenced throughout the text.

  1. {sup 14}C dating and isotopic composition of mangrove soil in the Parque Estadual da Ilha do Cardoso, Southern Sao Paulo State

    Energy Technology Data Exchange (ETDEWEB)

    Vidotto, Elaine; Pessenda, Luiz Carlos Ruiz; Gouveia, Susy Eli M.; Bendassolli, Jose Albertino [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mails: evidotto@cena.usp.br; pessenda@cena.usp.br; susyeli@cena.usp.br; jab@cena.usp.br; Ricardi-Branco, Fresia [Universidade Estadual de Campinas (UNICAMP), SP (Brazil)]. E-mail: fresia@ige.unicamp.br; Aravena, Ramon [University of Waterloo, Ontario (Canada)]. E-mail: roaraven@sciborg.uwaterloo.ca

    2007-07-01

    This work is part of a study involving paleoenvironmental records based on pollen analysis, isotopic composition ({sup 12}C, {sup 13}C and {sup 14}N, {sup 15}N) and {sup 14}C dating of mangrove soil. Samplings have been made in the Jacariu mangrove located at Parque Estadual da Ilha do Cardoso, Cananeia, southern Sao Paulo state. This study must improve significantly the studies of vegetation and climate changes that occurred during the late Holocene (approximately the last 2000 years) in the southeastern region of Brazil. The {delta}{sup 13}C results of mangrove soil presented values from -24 to -26 per mille The values of TOC and C/N associated with {delta}{sup 13}C values indicated the predominance of organic matter from C{sub 3} land plant and phytoplankton (algal) in the whole profile. The {sup 14}C dating indicated Modern age at the shallow horizons to 1810 years B.P. for the deeper horizon. (author)

  2. A preliminary study on the behavior of trace elements in sediment cores from Ilha Grande (Rio de Janeiro State) by neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Wasserman, Julio Cesar [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Geoquimica; Figueiredo, Ana Maria G. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil); Figueira, Andre Luiz [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Oceanografia; Kelecom, Alphonse [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Biologia Geral

    2002-07-01

    The present work aims to identify atmospheric and marine inputs of 9 metals (Ba, Co, Cr, Cs, Fe, Hf, Rb, Sc, Zn), 8 rare earths (La, Ce, Nd, Sm, Eu, Tb, Yb e Lu), 2 actinides (U, Th) and 3 non-metals (As, Sb, Se) in sediment cores from a remote area, the Biological Reserve of Praia do Sul, Ilha Grande, Rio de Janeiro, Brazil. The sediment cores were sampled in a peat bog (out of the tidal reach) and in a mangrove, downstream of the peat bog. The analytical technique employed was Instrumental Neutron Activation Analysis. The samples were irradiated for 16 hours at a thermal neutron flux of 10{sup 12} n cm{sup -2} s{sup -1} at the IEA-R1 reactor of IPEN. The measurements of the induced gamma-ray activity were carried out by high resolution gamma spectrometry, with an hyperpure Ge detector. A preliminary sediment dating with Po-210 was also carried out by applying radiochemical procedures and measurements were done in an Alfa spectrometer The results indicate that the peat bog core present a slight surface enrichment that can be attributed to atmospheric inputs. Increasing concentrations of metals with age is probably due to history of soil occupation. In the mangrove core, no significant increase in concentration could be detected in the surface sediments (except for Zn) confirming the suitability of the peat bog core as a tracer for atmospheric inputs. (author)

  3. A preliminary study on the behavior of trace elements in sediment cores from Ilha Grande (Rio de Janeiro State) by neutron activation analysis

    International Nuclear Information System (INIS)

    Wasserman, Julio Cesar; Figueira, Andre Luiz; Kelecom, Alphonse

    2002-01-01

    The present work aims to identify atmospheric and marine inputs of 9 metals (Ba, Co, Cr, Cs, Fe, Hf, Rb, Sc, Zn), 8 rare earths (La, Ce, Nd, Sm, Eu, Tb, Yb e Lu), 2 actinides (U, Th) and 3 non-metals (As, Sb, Se) in sediment cores from a remote area, the Biological Reserve of Praia do Sul, Ilha Grande, Rio de Janeiro, Brazil. The sediment cores were sampled in a peat bog (out of the tidal reach) and in a mangrove, downstream of the peat bog. The analytical technique employed was Instrumental Neutron Activation Analysis. The samples were irradiated for 16 hours at a thermal neutron flux of 10 12 n cm -2 s -1 at the IEA-R1 reactor of IPEN. The measurements of the induced gamma-ray activity were carried out by high resolution gamma spectrometry, with an hyperpure Ge detector. A preliminary sediment dating with Po-210 was also carried out by applying radiochemical procedures and measurements were done in an Alfa spectrometer The results indicate that the peat bog core present a slight surface enrichment that can be attributed to atmospheric inputs. Increasing concentrations of metals with age is probably due to history of soil occupation. In the mangrove core, no significant increase in concentration could be detected in the surface sediments (except for Zn) confirming the suitability of the peat bog core as a tracer for atmospheric inputs. (author)

  4. Potential bird dispersers of Psychotria in a area of Atlantic forest on Ilha Grande, RJ, Southeastern Brazil: a biochemical analysis of the fruits

    Directory of Open Access Journals (Sweden)

    E. M. Almeida

    Full Text Available The present study assessed the fruiting pattern, bird foraging behavior, and sugar content of ripe fruits of two sympatric species of Rubiaceae (Psychotria brasiliensis and P. nuda. This study was carried out in an Atlantic forest area on Ilha Grande, RJ, between August 1998 and July 1999. Fruit production occurred year round, with a peak of mature P. brasiliensis fruits in December 1998 and another of P. nuda in February of 1999. Lipaugus lanioides (Cotingidae, Baryphtengus ruficapillus (Momotidae and Saltator similis (Emberizidae made the most frequent foraging visits to fruiting P. brasiliensis, so that L. lanioides and B. ruficapillus removed the fruits with sallying maneuvers while S. similis gleaned the fruits. Lipaugus lanioides was by far the most important consumer, and potentially the main disperser of P. brasiliensis. Birds of this genus are heavy frugivores in the tropical forests and are widely assumed to be important seed dispersers. The fruits were analyzed quantitatively and qualitatively in relation to the amounts of sucrose and starch. Psychotria brasiliensis (the visited species showed the smallest quantity of sucrose and the highest amount of starch. These findings suggest that what may influence the birds' choice of fruit is the proportion of starch in the Psychotria species studied here rather than the carbohydrate composition.

  5. Plan Continental de ampliación e intensificación del combate a Aedes aegypti

    Directory of Open Access Journals (Sweden)

    1998-01-01

    Full Text Available Campaigns for the eradication of Aedes aegypti, the mosquito vector responsible for spreading dengue and yellow fever, enjoyed great success during the forties and fifties. Between 1948 and 1962, A. aegypti disappeared from 21 countries in the Region of the Americas, but lack of sustainability of the programs resulted in the gradual reinfestation of practically all countries. In an effort to combat the situation, in 1995 PAHO began to help its Member States with the creation of an expert panel charged with drawing up a continental plan of action for eradicating A. aegypti from all countries. The Continental Plan for expanding and intensifying the war against Aedes aegypti was drawn up in Caracas, Venezuela, in April of 1997, in accordance with the objectives previously established by the countries. The plan's success will depend on having all countries commit themselves to putting it into effect and to providing the national funds that are needed for its full implementation.

  6. Optimal barrier zones for stopping the invasion of Aedes aegypti mosquitoes via transgenic or sterile insect techniques

    KAUST Repository

    Lee, S. Seirin; Baker, Ruth E.; Gaffney, Eamonn A.; White, Steven M.

    2013-01-01

    (Release of Insects carrying a Dominant Lethal), for controlling invasion of the mosquito Aedes aegypti using a spatial stage-structured mathematical model. In particular, we explore the use of a barrier zone of sterile/transgenic insects to prevent

  7. A cross-sectional survey of Aedes aegypti immature abundance in urban and rural household containers in central Colombia.

    Science.gov (United States)

    Overgaard, Hans J; Olano, Víctor Alberto; Jaramillo, Juan Felipe; Matiz, María Inés; Sarmiento, Diana; Stenström, Thor Axel; Alexander, Neal

    2017-07-27

    Aedes aegypti, the major vector of dengue, breeds in domestic water containers. The development of immature mosquitoes in such containers is influenced by various environmental, ecological and socioeconomic factors. Urban and rural disparities in water storage practices and water source supply may affect mosquito immature abundance and, potentially, dengue risk. We evaluated the effect of water and container characteristics on A. aegypti immature abundance in urban and rural areas. Data were collected in the wet season of 2011 in central Colombia from 36 urban and 35 rural containers, which were either mosquito-positive or negative. Immature mosquitoes were identified to species. Data on water and container characteristics were collected from all containers. A total of 1452 Aedes pupae and larvae were collected of which 81% were A. aegypti and 19% A. fluviatilis. Aedes aegypti immatures were found in both urban and rural sites. However, the mean number of A. aegypti pupae was five times higher in containers in the urban sites compared to those in the rural sites. One of the important factors associated with A. aegypti infestation was frequency of container washing. Monthly-washed or never-washed containers were both about four times more likely to be infested than those washed every week. There were no significant differences between urban and rural sites in frequency of washing containers. Aedes aegypti immature infestation was positively associated with total dissolved solids, but negatively associated with dissolved oxygen. Water temperature, total dissolved solids, ammonia, nitrate, and organic matter were significantly higher in urban than in rural containers, which might explain urban-rural differences in breeding of A. aegypti. However, many of these factors vary substantially between studies and in their degree of association with vector breeding, therefore they may not be reliable indices for vector control interventions. Although containers in urban areas

  8. Development and Evaluation of a Pyriproxyfen-treated Device to Control the Dengue Vector, Aedes aegypti (L.) (Diptera: Culicidae)

    Science.gov (United States)

    2013-03-01

    aegypti females lay their eggs in many sites (Harrington and Ed- man, 2001; Reiter, 2007). This behavior im- proves likelihood of survival. Blood-fed Ae...coiled into skeins) or parous ( ovaries stretched and uncoiled) (Service, 1993). Blood-fed, gravid females were not dissected but classified as...deposition. Ishaaya and Horowitz (1992) found newly deposited eggs (0-1 day old) from female sweet- A PyriProxyfen TreATed device for Ae. Aegypti

  9. Comparison of BG-Sentinel Trap and Oviposition Cups for Aedes aegypti and Aedes albopictus Surveillance in Jacksonville, Florida, USA

    Science.gov (United States)

    2015-03-01

    COMPARISON OF BG-SENTINELH TRAP AND OVIPOSITION CUPS FOR AEDES AEGYPTI AND AEDES ALBOPICTUS SURVEILLANCE IN JACKSONVILLE, FLORIDA, USA JENNIFER A...trap and oviposition cups (OCs) have both proven effective in the surveillance of Aedes species. This study aimed to determine which of the 2 traps could...best characterize the relative population sizes of Aedes albopictus and Aedes aegypti in an urban section of Jacksonville, FL. Until 1986, Ae

  10. Updated Reported Distribution of Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus (Diptera: Culicidae) in the United States, 1995-2016.

    Science.gov (United States)

    Hahn, Micah B; Eisen, Lars; McAllister, Janet; Savage, Harry M; Mutebi, John-Paul; Eisen, Rebecca J

    2017-09-01

    Aedes (Stegomyia) aegypti (L.) and Aedes (Stegomyia) albopictus (Skuse) are potential vectors of Zika, dengue, and chikungunya viruses in the United States. A Zika virus outbreak in Florida in the summer of 2016, driven by Ae. aegypti and resulting in > 200 locally acquired cases of human illness, underscored the need for up-to-date information on the geographic distribution of Ae. aegypti and Ae. albopictus in the United States. In early 2016, we conducted a survey and literature review to compile county records for presence of Ae. aegypti and Ae. albopictus in the United States from 1995 to 2016. Surveillance for these vectors was intensified across the United States during the summer and fall of 2016. At the end of 2016, we therefore conducted a follow-up survey of mosquito control agencies, university researchers, and state and local health departments to document new collection records for Ae. aegypti and Ae. albopictus. The repeated survey at the end of the year added Ae. aegypti collection records from 38 new counties and Ae. albopictus collection records from 127 new counties, representing a 21 and 10 percent increase, respectively, in the number of counties with reported presence of these mosquitoes compared with the previous report. Moreover, through our updated survey, 40 and 183 counties, respectively, added additional years of collection records for Ae. aegypti and Ae. albopictus from 1995 to 2016. Our findings underscore the continued need for systematic surveillance of Ae. aegypti and Ae. albopictus. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.

  11. Septic tanks as larval habitats for the mosquitoes Aedes aegypti and Culex quinquefasciatus in Playa-Playita, Puerto Rico.

    Science.gov (United States)

    Burke, R; Barrera, R; Lewis, M; Kluchinsky, T; Claborn, D

    2010-06-01

    Adult Aedes aegypti (Linnaeus) (Diptera: Culicidae) were previously recovered from emergence traps on septic tanks in southeastern Puerto Rico. In this study we quantified immature mosquito abundance and its relationship with structural variables of the septic tanks and chemical properties of the water containing raw sewage. A miniaturized floating funnel trap was used to sample 89 septic tanks for larvae in the Puerto Rican community of Playa-Playita. Aedes aegypti larvae were recovered from 18% of the sampled tanks (10.3 larvae per septic tank per day). Larval presence was positively associated with cracking of the septic tank walls and uncovered access ports. Larval abundance was positively associated with cracking of the septic tank walls and larger tank surface areas, and inversely associated with the total dissolved solids (TDS). Culex quinquefasciatus (Say) larvae were also recovered from 74% of the septic tanks (129.6 larvae per septic tank per day). Larval presence was negatively associated with TDS in the water and larval abundance was positively associated with cracking of the septic tank walls. A screened, plastic emergence trap was used to sample 93 septic tanks within the community for Ae. aegypti and Cx. quinquefasciatus adults. Aedes aegypti adults were recovered from 49% of the sampled tanks (8.7 adults per septic tank per day) and Cx. quinquefasciatus adults were recovered from 97% of the sampled tanks (155.5 adults per septic tank per day). Aedes aegypti adult presence was positively associated with cracking, uncapped openings and septic water pH. The Ae. aegypti adult counts were positively associated with cracking and inversely associated with TDS and conductivity. This study marks the first published record of the recovery of Ae. aegypti larvae from holding tanks containing raw sewage in the Caribbean region. Our study indicates that Ae. aegypti larvae are present in sewage water and that septic tanks have at least the potential to maintain

  12. The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed

    OpenAIRE

    Maciel-de-Freitas, Rafael; Sylvestre, Gabriel; Gandini, Mariana; Koella, Jacob C.

    2013-01-01

    BACKGROUND: Dengue virus (DENV) is transmitted by Aedes aegypti, a species that lives in close association with human dwellings. The behavior of DENV-infected mosquitoes needs further investigation, especially regarding the potential influence of DENV on mosquito biting motivation and avidity. METHODOLOGY/PRINCIPAL FINDINGS: We orally challenged 4-5 day-old Ae. aegypti females with a low passage DENV serotype -2 (DENV-2) to test whether the virus influences motivation to feed (the likelihood ...

  13. Knockdown resistance (kdr) of the voltage-gated sodium channel gene of Aedes aegypti population in Denpasar, Bali, Indonesia.

    Science.gov (United States)

    Hamid, Penny Humaidah; Prastowo, Joko; Widyasari, Anis; Taubert, Anja; Hermosilla, Carlos

    2017-06-05

    Aedes aegypti is the main vector of several arthropod-borne viral infections in the tropics profoundly affecting humans, such as dengue fever (DF), West Nile (WN), chikungunya and more recently Zika. Eradication of Aedes still largely depends on insecticides, which is the most cost-effective strategy, and often inefficient due to resistance development in exposed Aedes populations. We here conducted a study of Ae. aegypti resistance towards several insecticides regularly used in the city of Denpasar, Bali, Indonesia. Aedes aegypti egg samples were collected with ovitraps and thereafter hatched in the insectary of the Gadjah Mada University. The F0 generation was used for all bioassay-related experiments and knockdown resistance (kdr) assays. Results clearly showed resistance development of Ae. aegypti against tested insecticides. Mortalities of Ae. aegypti were less than 90% with highest resistance observed against 0.75% permethrin. Mosquitoes from the southern parts of Denpasar presented high level of resistance pattern in comparison to those from the western and northern parts of Denpasar. Kdr analysis of voltage-gated sodium channel (Vgsc) gene showed significant association to S989P and V1016G mutations linked to resistance phenotypes against 0.75% permethrin. Conversely, Ae. aegypti F1534C gene mutation did not result in any significant correlation to resistance development. Periodically surveillance of insecticide resistances in Ae. aegypti mosquitoes will help local public health authorities to set better goals and allow proper evaluation of on-going mosquito control strategies. Initial detection of insecticide resistance will contribute to conduct proper actions in delaying mosquito resistance development such as insecticide rotation or combination of compounds in order to prolong chemical efficacy in combating Ae. aegypti vectors in Indonesia.

  14. Effects of insemination and blood-feeding on locomotor activity of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) females under laboratory conditions.

    Science.gov (United States)

    Lima-Camara, Tamara Nunes; Lima, José Bento Pereira; Bruno, Rafaela Vieira; Peixoto, Alexandre Afranio

    2014-07-02

    Dengue is an arbovirus disease transmitted by two Aedes mosquitoes: Ae. aegypti and Ae. albopictus. Virgin females of these two species generally show a bimodal and diurnal pattern of activity, with early morning and late afternoon peaks. Although some studies on the flight activity of virgin, inseminated and blood-fed Ae. aegypti females have been carried out under laboratory conditions, little is known about the effects of such physiological states on the locomotor activity of Ae. albopictus and Ae. aegypti females. The aim of this study was to analyze, under laboratory conditions, the effects of insemination and blood-feeding on the locomotor activity of Ae. albopictus and Ae. aegypti females under LD 12:12, at 25°C. Both Ae. albopictus and Ae. aegypti females were obtained from established laboratory colonies. Control groups were represented by virgin/unfed Ae. albopictus and Ae. aegypti females. Experiments were conducted under laboratory conditions, using an activity monitor that registers individual activity every thirty minutes. Virgin/unfed Ae. albopictus and Ae. aegypti females showed a diurnal and bimodal pattern of locomotor activity, with peaks at early morning and late afternoon. Insemination and blood-feeding significantly decreased the locomotor activity of Ae. aegypti females, but inseminated/blood-fed Ae. aegypti and Ae. albopictus females showed a similar significant decrease on the locomotor activity compared to virgin/unfed females. This study is the first demonstration of the effects of insemination and blood-feeding on the locomotor activity of Ae. albopictus and Ae. aegypti females under artificial conditions. Data suggest that Ae. albopictus and Ae. aegypti females respond in different ways to physiological status changes and such divergence between these two dengue vectors, associated with several ecological differences, could be related to the greater dengue vectorial capacity of Ae. aegypti in Americas in comparison to Ae. albopictus.

  15. Composition of the Essential Oil of Pink Chablis Bluebeard (Caryopteris x clandonensis ’Durio’) and Its Biological Activity against the Yellow Fever Mosquito Aedes aegypti

    Science.gov (United States)

    2016-04-07

    oil of Pink Chablis™ bluebeard (Caryopteris ×clandonensis ’Durio’) and its biological activity against the yellow fever mosquito Aedes aegypti ARTICLE...bluebeard (Caryopteris ×clandonensis ’Durio’) and its biological activity against the yellow fever mosquito Aedes aegypti Eugene K. Blythe1...mosquito [ Aedes aegypti (L.) (Diptera: Culicidae)]. Essential oil from the aerial parts of this mildly aromatic ornamental species was extracted by water

  16. Impact of Diurnal Temperature Fluctuations during Larval Development on Adult Life History Traits and Insecticide Susceptibility in Two Vectors; Anopheles gambiae and Aedes aegypti

    Science.gov (United States)

    2014-04-30

    ANOPHELES GAMBIAE AND AEDES AEGYPTI. by Jeffrey W. Clark Dissertation submitted to the Faculty of the Department of Preventive Medicine and...Vectors; Anopheles gambiae and Aedes aegypti." Name of Candidate: Jeffrey Clark Doctor of Philosophy Degree April 30, 2014 DISSERTATION AND ABSTRACT...for the many fruitful discussions and the standing offer to help whenever I needed it; and to Joe Wagman, for providing needed Aedes aegypti eggs from

  17. Efectos de la competencia larval en los mosquitos de contenedores artificiales, Aedes aegypti y Culex pipiens (Diptera: Culicidae en condiciones semi-controladas

    Directory of Open Access Journals (Sweden)

    Analía FRANCIA

    2011-01-01

    Full Text Available Las larvas de los mosquitos Aedes aegypti (Linneo y Culex pipiens Linneo pueden criar conjuntamente en pequeños contenedores artificiales de agua, se genera así una competencia interespecífica y/o intraespecífica. El objetivo de este trabajo fue comparar la magnitud relativa de la competencia intra e interespecífica en A. aegypti y C. pipiens, generada durante el desarrollo larval en contenedores artificiales. Las variables medidas como respuesta fueron la supervivencia y el tiempo de desarrollo larval, y la biomasa total producida en estado de pupa. Se criaron larvas de ambos mosquitos en neumáticos de automóvil con agua declorinada y hojarasca. Se introdujeron larvas recién eclosionadas de acuerdo a la densidad (δ estimada según un censo previo de A. aegypti y C. pipiens. Se realizaron los siguientes tratamientos agregando larvas de: (1 A. aegypti hasta alcanzar δ A. aegypti determinada según el censo previo, (2 C. pipiens hasta δ C. pipiens del censo previo, (3 A. aegypti hasta alcanzar la suma de δ A. aegypti y δ C. pipiens del censo previo, (4 C. pipiens hasta alcanzar la suma de δ A. aegypti y δ C. pipiens del censo previo y (5 A. aegypti y C. pipiens hasta δ A. aegypti y δ C. pipiens del censo previo. Las tres variables medidas fueron afectadas por los tratamientos, excepto la supervivencia y la biomasa producida por C. pipiens. Aedes aegypti fue más alterada por la competencia intraespecífica que por la competencia interespecífica. En C. pipiens, la competencia interespecífica superó en sus efectos a la competencia intraespecífica. Existió asimetría competitiva, ya que C. pipiens fue más afectada por A. aegypti que lo contrario.

  18. Imaginal discs--a new source of chromosomes for genome mapping of the yellow fever mosquito Aedes aegypti.

    Directory of Open Access Journals (Sweden)

    Maria V Sharakhova

    2011-10-01

    Full Text Available The mosquito Aedes aegypti is the primary global vector for dengue and yellow fever viruses. Sequencing of the Ae. aegypti genome has stimulated research in vector biology and insect genomics. However, the current genome assembly is highly fragmented with only ~31% of the genome being assigned to chromosomes. A lack of a reliable source of chromosomes for physical mapping has been a major impediment to improving the genome assembly of Ae. aegypti.In this study we demonstrate the utility of mitotic chromosomes from imaginal discs of 4(th instar larva for cytogenetic studies of Ae. aegypti. High numbers of mitotic divisions on each slide preparation, large sizes, and reproducible banding patterns of the individual chromosomes simplify cytogenetic procedures. Based on the banding structure of the chromosomes, we have developed idiograms for each of the three Ae. aegypti chromosomes and placed 10 BAC clones and a 18S rDNA probe to precise chromosomal positions.The study identified imaginal discs of 4(th instar larva as a superior source of mitotic chromosomes for Ae. aegypti. The proposed approach allows precise mapping of DNA probes to the chromosomal positions and can be utilized for obtaining a high-quality genome assembly of the yellow fever mosquito.

  19. LOTION PREPARATION FROM Piper betle L. ESSENTIAL OIL WITH THE ADDITION OF PATCHOULI OIL AS AN Aedes aegypti REPELLENT

    Directory of Open Access Journals (Sweden)

    Mutiara Widawati

    2015-01-01

    Full Text Available Daun Sirih (Piper betle L. merupakan tanaman yang dapat dimanfaatkan sebagai bahan baku repelan. Penelitian bertujuanuntuk mengetahui potensi minyak atsiri dari daun sirih dengan penambahan minyak nilam sebagai repelan. Penelitianeksperimental dengan rancangan post test only control group design dilakukan tahun 2013, menggunakan sampel nyamukAe. aegypti betina lapar darah. Konsentrasi digunakan yaitu 2%, untuk kontrol positif digunakan losion DEET denganulangan lima kali. Lengan diolesi losion sirih selanjutnya dimasukkan pada kurungan berisi 100 ekor nyamuk uji, kemudiandihitung rata-rata jumlah nyamuk hinggap selama lima menit pengamatan setiap jam periode (uji efikasi repelan dilakukanselama 6 jam. Pada kondisi yang sama, diujikan pula losion biasa tanpa minyak sirih dan fiksatif yang dioleskan ke lenganyang lain terhadap nyamuk Ae. aegypti (kontrol negatif. Efektifitas penolakan hinggapan nyamuk Ae. aegypti dianalisismenggunakan daya proteksi, kemudian dianalisis lebih lanjut dengan uji paired t-test. Losion sirih hasil modifikasi yangdioleskan pada lengan mampu menolak hinggapan nyamuk Ae. aegypti. Losion sirih dengan penambahan minyak nilammemiliki daya proteksi rata-rata 90,33%. Walaupun daya proteksi losion sirih tidak berbeda secara nyata dengan dayaproteksi DEET, tetapi masih memenuhi syarat efektivitas repelan. Minyak sirih dengan penambahan minyak nilamberpotensi untuk digunakan sebagai repelan terhadap nyamuk Ae. aegypti.Kata kunci: losion, daun sirih, minyak nilam, repelan, Aedes aegypti

  20. First report on invasion of yellow fever mosquito, Aedes aegypti, at Narita International Airport, Japan in August 2012.

    Science.gov (United States)

    Sukehiro, Nayu; Kida, Nori; Umezawa, Masahiro; Murakami, Takayuki; Arai, Naoko; Jinnai, Tsunesada; Inagaki, Shunichi; Tsuchiya, Hidetoshi; Maruyama, Hiroshi; Tsuda, Yoshio

    2013-01-01

    The invasion of the yellow fever mosquito Aedes aegypti at Narita International Airport, Japan was detected for the first time. During the course of routine vector surveillance at Narita International Airport, 27 Ae. aegypti adults emerged from larvae and pupae collected from a single larvitrap placed near No. 88 spot at passenger terminal 2 on August 8, 2012. After the appearance of Ae. aegypti in the larvitrap, we defined a 400-m buffer zone and started an intensive vector survey using an additional 34 larvitraps and 15 CO2 traps. International aircraft and passenger terminal 2 were also inspected, and one Ae. aegypti male was collected from the cargo space of an international aircraft from Darwin via Manila on August 28, 2012. Larvicide treatment with 1.5% fenitrothion was conducted in 64 catch basins and one ditch in the 400-m buffer zone. Twenty-four large water tanks were also treated at least once with 0.5% pyriproxyfen, an insect growth regulator. No Ae. aegypti eggs or adults were found during the 1-month intensive vector survey after finding larvae and pupae in the larvitrap. We concluded that Ae. aegypti had failed to establish a population at Narita International Airport.

  1. Age-Stage, Two-Sex Life Table Characteristics of Aedes albopictus and Aedes Aegypti in Penang Island, Malaysia.

    Science.gov (United States)

    Maimusa, Hamisu A; Ahmad, Abu Hassan; Kassim, Nur Faeza A; Rahim, Junaid

    2016-03-01

    The life table developmental attributes of laboratory colonies of wild strains of Aedes albopictus and Aedes aegypti were analyzed and compared based on the age-stage, two-sex life table. Findings inclusive in this study are: adult preoviposition periods, total preoviposition period, mean intrinsic rate of increase (r), mean finite rate of increase (λ), net reproductive rates (R0), and mean generation time (T). The total preadult development time was 9.47 days for Ae. albopictus and 8.76 days for Ae. aegypti. The life expectancy was 19.01 days for Ae. albopictus and 19.94 days for Ae. aegypti. Mortality occurred mostly during the adult stage. The mean development time for each stage insignificantly correlated with temperature for Ae. albopictus (r  =  -0.208, P > 0.05) and (r  =  -0.312, P > 0.05) for Ae. aegypti. The population parameters suggest that Ae. albopictus and Ae. aegypti populations are r-strategists characterized by a high r, a large R0, and short T. This present study provides the first report to compare the life parameters of Ae. albopictus and Ae. aegypti strains from Penang island, Malaysia.

  2. Genetic structure and phylogeography of Aedes aegypti, the dengue and yellow-fever mosquito vector in Bolivia.

    Science.gov (United States)

    Paupy, Christophe; Le Goff, Gilbert; Brengues, Cécile; Guerra, Mabel; Revollo, Jimmy; Barja Simon, Zaïra; Hervé, Jean-Pierre; Fontenille, Didier

    2012-08-01

    Between the 16th and 18th centuries, Aedes aegypti (Diptera: Culicidae), a mosquito native to Africa, invaded the Americas, where it was successively responsible for the emergence of yellow fever (YF) and dengue (DEN). The species was eradicated from numerous American countries in the mid-20th century, but re-invaded them in the 1970s and 1980s. Little is known about the precise identities of Ae. aegypti populations which successively thrived in South America, or their relation with the epidemiological changes in patterns of YF and DEN. We examined these questions in Bolivia, where Ae. aegypti, eradicated in 1943, re-appeared in the 1980s. We assessed the genetic variability and population genetics of Ae. aegypti samples in order to deduce their genetic structure and likely geographic origin. Using a 21-population set covering Bolivia, we analyzed the polymorphism at nine microsatellite loci and in two mitochondrial DNA regions (COI and ND4). Microsatellite markers revealed a significant genetic structure among geographic populations (F(ST)=0.0627, PBolivia. Analysis of mtDNA sequences revealed the existence of two genetic lineages, one dominant lineage recovered throughout Bolivia, and the second restricted to rural localities in South Bolivia. Phylogenic analysis indicated that this minority lineage was related to West African Ae. aegypti specimens. In conclusion, our results suggested a temporal succession of Ae. aegypti populations in Bolivia, that potentially impacted the epidemiology of dengue and yellow fever. Copyright © 2012 Elsevier B.V. All rights reserved.

  3. Potensi Serbuk Daun Sirih (piper betle, Linn Sebagai Larvasida Nyamuk Aedes aegypti

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    Betriyon Betriyon

    2017-02-01

    Full Text Available Abstract Larvacide was compound/essence used to kill  larvae stadium. Many people used Piper betle to traditional medicine. Piper betle plant L,.has contents bioactive compound as flavonoid compound, atsiri volatile, polifenol, tannin, alkaloid and saponin which have quality as larvacide. Based on the case above, this research aim to put the experiment Piper betle of the Aedes aegypti mosquito instars IV. This was pure experiment research using complete random design. This research was done at laboratory . Larva used was  Aedes aegypti mosquito instars IV. Concentration used of this research was : 0,1; 0,2; 0,3; 0,4; 05 percent b/v. This research has control (+ was temephos 0,0001 persen, control (- was aquades water not added anything. Observation done every hour up to all the larva death with the replication 5 times. Larva death cumulative data on 10th hour and 24 th used to calculate LC50 and LT50 used probit regression analysis than data analyzed using Levene’s test. The result of this research showed that the cost of LC50 piper betle plant L. on 10th hour was 0,54 ± 0,147 persen b/v, than on 24 hour LC50 on concentration 0,07 up to 0,28 percent b/v. the time needed to cross out 50 percent Aedes aegypti instar IV of the concentration 0,05 percent time needed 19. Based on a probit analysis of the relationship between the level of concentration with the number of larvae mortality, Piper betle have activities as larvacide of Aedes aegypti. Keywods: piper betle leaf powder, larvacide, Aedes aegyti AbstrakLarvasida adalah senyawa/zat yang digunakan untuk membunuh stadium larva. Tanaman Piper betle, Linn. (sirih hijau sudah banyak dimanfaatkan oleh kalangan masyarakat sebagai obat tradisional. Daun sirih hijau memiliki kandungan senyawa bioaktif seperti senyawa flavonoid, minyak atsiri, polifenol, tannin, alkaloid dan saponin yang dapat bersifat sebagai larvasida. Penelitian ini bertujuan untuk mengetahui manfaat daun sirih hijau yaitu

  4. Preferensi Aedes aegypti Meletakkan Telur pada Berbagai Warna Ovitrap di Laboratorium

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    Made Agus Nurjana

    2017-07-01

    Full Text Available ABSTRACTDengue Haemograffic fever is a vector borne disease which caused outbreaks and death. There is no applied vaccine until now, so the effort of prevention and control is to terminate chain of infection mosquito breeding. Factors which influenced  the female mosquitoe to lay their eggsare type of container, color, water, temperature, water source, humidity and environment condition. This study was conducted to determine the preferences of Ae. aegypti mosquitoes to lay aggs in various colors ovitrap in the laboratory of Balai Litbang P2B2 Donggala, January until March 2015. Three repetitions with plastic cup black, blue, white, yellow and pink have been performed with water and filter pappers. 30 mosquitoes blood saturation included in the containers with various colors. The result showed that most of female mosquito laid their eggs in plastic cup black (53,2%. ANOVA analysis showed that the diversity of colors ovitrap produce different the number of eggs Ae. aegypti in each type of countainer color. It is recommended to use black ovitrap for controling populations of Ae, aegypti in environment with regular monitoring.Demam Berdarah Dengue merupakan penyakit yang sering menimbulkan wabah dan dapat menyebabkan kematian. Sampai saat ini belum ditemukan vaksin sehingga pemberantasannya masih didasarkan pada pemutusan mata rantai penularan seperti pemberantasan sarang nyamuk. Beberapa faktor yang mempengaruhi proses bertelur nyamuk antara lain adalah jenis wadah, warna wadah, air, suhu, sumber air, kelembaban dan kondisi lingkungan. Penelitian ini bertujuan  untuk mengetahui preferensi nyamuk Ae. aegypti untuk meletakkan telur pada berbagai warna ovitrap di Laboratorium Balai Litbang P2B2 Donggala bulan Januari sampai Maret 2015. Tiga kali pengulangan dengan mangkok plastik yang berwarna hitam, hijau, biru, putih, kuning dan merah muda. Nyamuk jenuh darah sebanyak 30 ekor dimasukkan kedalam kandang yang berisi mangkok plastik berbagai warna

  5. Tingkat Kerentanan Aedes aegypti (Linn. terhadap Malation di Provinsi Sumatera Selatan

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    Lasbudi P. Ambarita

    2015-07-01

    Full Text Available AbstractDengue vector control program in Indonesia and also South Sumatera Province has been using malathion quite long enough. The extensive use of chemical in dengue vector control can lead to development of resistance. This study aims to determine the susceptibility of Aedes aegypti against malathion in 11 district of South Sumatera Province. Larva or pupae were collected with entomology survey kit and colonized until first generation (F1 that were used for bioassay. This test was conducted according to WHO adult susceptibility bioassay procedure.Twenty five blood-fed mosquitoes were exposed to insecticide impregnated paper in each of 4 WHO test kits and 1 control tube. Aedes aegypti from all study sites were still susceptible to operational dose of malathion (5%after 1 hour exposure. The estimated resistance ratio (ERR of knockdown time (KT to operasional dose of malathion is about 1,02 – 1,27 for KT50 and 0,96 – 1,24 for KT95. The susceptibility test of adult mosquitoes to diagnostic dose (0,8% of malathion showed a variety of susceptibility after 24 hours. Strain of 7 districts showed resistance, 3 districts toleran and 1 district still susceptible. The detection of resistance can actually help public health personnel to formulate appropriate steps in encountering the reduction in effectiveness of vector control efforts.Keywords : Aedes aegypti, Malathion, Susceptibility, South SumateraAbstrakProgram pengendalian vektor DBD di Indonesia termasuk di Provinsi Sumatera Selatan telah cukup lama menggunakan malation dengan konsentrasi 5%. Penggunaan satu jenis insektisida kimiawi secara ekstensif dapat memicu perkembangan resistensi. Penelitian ini bertujuan untuk menentukan status kerentanan Aedes aegypti terhadap malation dari 11 kabupaten/kota di Provinsi Sumatera Selatan. Larva atau pupa dikumpulkan menggunakan alat survei entomologi dan selanjutnyadipelihara hingga mendapatkan generasi pertama (F1 yang akan digunakan pada uji

  6. Susceptibility profile of Aedes aegypti from Santiago Island, Cabo Verde, to insecticides.

    Science.gov (United States)

    Rocha, Hélio Daniel Ribeiro; Paiva, Marcelo Henrique Santos; Silva, Norma Machado; de Araújo, Ana Paula; Camacho, Denise Dos Reis da Rosa de Azevedo; Moura, Aires Januário Fernandes da; Gómez, Lara Ferrero; Ayres, Constância Flávia Junqueira; Santos, Maria Alice Varjal de Melo

    2015-12-01

    In 2009, Cabo Verde diagnosed the first dengue cases, with 21,137 cases reported and Aedes aegypti was identified as the vector. Since the outbreak, chemical insecticides and source reduction were used to control the mosquito population. This study aimed to assess the susceptibility of A. aegypti populations from Santiago, Cabo Verde to insecticides and identify the mechanisms of resistance. Samples of A. aegypti eggs were obtained at two different time periods (2012 and 2014), using ovitraps in different locations in Santiago Island to establish the parental population. F1 larvae were exposed to different concentrations of insecticides (Bacillus thuringiensis var israelensis (Bti), diflubenzuron and temephos) to estimate the lethal concentrations (LC90) and calculate the respective rate of resistance (RR90). Semi-field tests using temephos-ABATE(®) were performed to evaluate the persistence of the product. Bottle tests using female mosquitoes were carried out to determine the susceptibility to the adulticides malathion, cypermethrin and deltamethrin. Biochemical and molecular tests were performed to investigate the presence of metabolic resistance mechanisms, associated with the enzymes glutathione S-transferases (GSTs), esterases and mixed-function oxidases (MFO) and to detect mutations or alterations in the sodium channel and acetylcholinesterase genes. A. aegypti mosquitoes from Santiago exhibited resistance to deltamethrin, cypermethrin (mortality<80%) and temephos (RR90=4.4) but susceptibility to malathion (mortality≥98%), Bti and diflubenzuron. The low level of resistance to temephos did not affect the effectiveness of Abate(®). The enzymatic analysis conducted in 2012 revealed slight changes in the activities of GST (25%), MFO (18%), α-esterase (19%) and β-esterase (17%), but no significant changes in 2014. Target site resistance mutations were not detected. Our results suggest that the A. aegypti population from Santiago is resistant to two major

  7. Repellent activity of herbal essential oils against Aedes aegypti (Linn. and Culex quinquefasciatus (Say.

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    Duangkamon Sritabutra

    2013-08-01

    Full Text Available Objective: To determine the mosquito repellent activity of herbal essential oils against female Aedes aegypti and Culex quinquefasciatus. Methods: On a volunteer’s forearm, 0.1 mL of each essential oil was applied to 3 cm伊10 cm of exposed skin. The protection time was recorded for 3 min after every 30 min. Results: Essential oil from clove oil in olive oil and in coconut oil gave the longest lasting period of 76.50 min and 96.00 min respectively against Aedes aegypti. The citronella grass oil in olive oil, citronella grass oil in coconut oil and lemongrass oil in coconut oil exhibited protection against Culex quinquefasciatus at 165.00, 105.00, and 112.50 min respectively. Conclusions: The results clearly indicated that clove, citronella and lemongrass oil were the most promising for repellency against mosquito species. These oils could be used to develop a new formulation to control mosquitoes.

  8. Influence of breeding site availability on the oviposition behaviour of Aedes aegypti

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    Filipe Vieira Santos de Abreu

    2015-08-01

    Full Text Available Despite the importance of the mosquito Aedes aegypti in the transmission of arboviruses, such as yellow fever, Chikungunya fever and dengue fever, some aspects of their behaviour remain unknown. In the present study, the oviposition behaviour of Ae. aegypti females that were exposed to different densities of breeding sites (2, 4, 8 and 16 was evaluated in laboratory and semi-field conditions. The number of breeding sites that were used was proportional to the number available, but tended towards stabilisation. Females used four-six breeding sites on average, with a maximum of 11. A high percentage of eggs was observed in the water, along with the presence of a breeding site termed “favourite”, which received at least 40% of the eggs. The results are discussed in ecological, evolutionary and epidemiological approaches.

  9. Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.

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    Roya Elaine Haghighat-Khah

    Full Text Available Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests.

  10. Leaking Containers: Success and Failure in Controlling the Mosquito Aedes aegypti in Brazil.

    Science.gov (United States)

    Löwy, Ilana

    2017-04-01

    In 1958, the Pan American Health Organization declared that Brazil had successfully eradicated the mosquito Aedes aegypti, responsible for the transmission of yellow fever, dengue fever, chikungunya, and Zika virus. Yet in 2016 the Brazilian minister of health described the situation of dengue fever as "catastrophic." Discussing the recent epidemic of Zika virus, which amplified the crisis produced by the persistence of dengue fever, Brazil's president declared in January 2016 that "we are in the process of losing the war against the mosquito Aedes aegypti." I discuss the reasons for the failure to contain Aedes in Brazil and the consequences of this failure. A longue durée perspective favors a view of the Zika epidemic that does not present it as a health crisis to be contained with a technical solution alone but as a pathology that has the persistence of deeply entrenched structural problems and vulnerabilities.

  11. Indeks Pertumbuhan Larva Aedes aegypti L. Yang Terdedah Dalam Ekstrak Air Kulit Jengkol (Pithecellobium lobatum

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    Firda Yanuar Pradani

    2013-02-01

    Full Text Available Abstracts. The control of dengue fever (DBD vector generally use the synthetic insecti­cides, however it's utilization had negative effect to the environment. Jengkol (Pithecellobium lobaturn was one of the plants which could be used as larvacide alternative because it had phenolate acid, alkaloid, terpenoid and saponin in its rind. This research was conducted to know the growth index of Aedes aegypti larvae which was soaked in the extract ofP. lobaturn rind. This experiment used P. lobaturn rind ekstract at concentration 36%, 18%, 9% and 0% . The data were analyzed by using Zhang et.al methode (1993. The P. lobaturn rind extract were toxic to larvae especially in first and second instar at concentration 17,94% respectively. Relativelly growth index range from 0,817-1, it is mean that some larvae were stuned, and some were growth into the next phase.Key words: Aedes aegypti, Jengkol, LCso, growth index.

  12. Chemical Composition of Buddleja polystachya Aerial Parts and its Bioactivity against Aedes aegypti.

    Science.gov (United States)

    El-Gamal, Ali; Al-Massarani, Shaza; Fawzy, Ghada; Ati, Hanan; Al-Rehaily, Adnan; Basudan, Omer; Abdel-Kader, Maged; Tabanca, Nurhayat; Becnel, James

    2017-09-25

    A new acylatediridoid glycoside, 6-O-α-L-(2″-acetyl-4″-O-trans-isoferuloyl) rhamnopyranosyl catalpol (9) together with 18 known compounds belonging to the iridoids, flavonoids, triterpene saponin glycosides and phenylethanoids (1-8, 10-18) were isolated from the aerial parts and the flowers of Buddleja polystachya. Their structures were elucidated on the basis of spectroscopic evidence and comparison with that reported in the literature. Promising adulticidal activity was shown for all extracts when tested for adulticidal and larvicidal activities against Ae. aegypti mosquitoes. Therefore, isolated compounds (1-10, 12-14 and 19) were bioassayed for their adulticidal activity. Compound 1 (phytol) was highly active with an LD 50 value of 1.27 ± 0.08 μg/mosquito against adult female Ae. aegypti.

  13. Influence of breeding site availability on the oviposition behaviour of Aedes aegypti

    Science.gov (United States)

    de Abreu, Filipe Vieira Santos; Morais, Maira Moreira; Ribeiro, Sérvio Pontes; Eiras, Álvaro Eduardo

    2015-01-01

    Despite the importance of the mosquito Aedes aegypti in the transmission of arboviruses, such as yellow fever, Chikungunya fever and dengue fever, some aspects of their behaviour remain unknown. In the present study, the oviposition behaviour of Ae. aegypti females that were exposed to different densities of breeding sites (2, 4, 8 and 16) was evaluated in laboratory and semi-field conditions. The number of breeding sites that were used was proportional to the number available, but tended towards stabilisation. Females used four-six breeding sites on average, with a maximum of 11. A high percentage of eggs was observed in the water, along with the presence of a breeding site termed “favourite”, which received at least 40% of the eggs. The results are discussed in ecological, evolutionary and epidemiological approaches. PMID:26154742

  14. Wolbachia Blocks Currently Circulating Zika Virus Isolates in Brazilian Aedes aegypti Mosquitoes.

    Science.gov (United States)

    Dutra, Heverton Leandro Carneiro; Rocha, Marcele Neves; Dias, Fernando Braga Stehling; Mansur, Simone Brutman; Caragata, Eric Pearce; Moreira, Luciano Andrade

    2016-06-08

    The recent association of Zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the Zika vector, Aedes aegypti mosquitoes. Wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. Here we report that Aedes aegypti harboring Wolbachia are highly resistant to infection with two currently circulating Zika virus isolates from the recent Brazilian epidemic. Wolbachia-harboring mosquitoes displayed lower viral prevalence and intensity and decreased disseminated infection and, critically, did not carry infectious virus in the saliva, suggesting that viral transmission was blocked. Our data indicate that the use of Wolbachia-harboring mosquitoes could represent an effective mechanism to reduce Zika virus transmission and should be included as part of Zika control strategies. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  15. HPTLC analysis of Scoparia dulcis Linn (Scrophulariaceae) and its larvicidal potential against dengue vector Aedes aegypti.

    Science.gov (United States)

    Wankhar, Wankupar; Srinivasan, Sakthivel; Rathinasamy, Sheeladevi

    2015-01-01

    This study evaluates the larvicidal activity of Scoparia dulcis aqueous extract against dengue vector and determines its major chemical components. The extract was tested at various concentrations ranging from 0.1 to 2 mg/mL against Aedes aegypti larvae. The extracts displayed significant larvicidal efficacy against Ae. aegypt species after 24 h exposure revealing LC50 of 3.3835 (mg/mL) and LC90 of 5.7578 (mg/mL). Finger printing profile carried out by CAMAG automatic TLC sample applicator programmed through WIN CATS software revealed peaks with different Rf values for three different volumes injected: 16, 15 and 18 peaks were spotted for 3, 6 and 9 μL, respectively. Ascending order of Rf values was also ascertained for each peak recorded. This study clearly signifies that S. dulcis extract contains numerous compounds that are known to have larvicidal properties which clearly substantiates its efficacy on Ae. aegypti larvae.

  16. Site-Specific Cassette Exchange Systems in the Aedes aegypti Mosquito and the Plutella xylostella Moth

    Science.gov (United States)

    Haghighat-Khah, Roya Elaine; Scaife, Sarah; Martins, Sara; St John, Oliver; Matzen, Kelly Jean; Morrison, Neil; Alphey, Luke

    2015-01-01

    Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE) mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests. PMID:25830287

  17. Aedes Aegypti en zona rural del municipio de La Mesa (Cundinamarca Colombia, S. A.

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    Alberto Morales

    1981-12-01

    Full Text Available Es bien sabida la importancia del mosquito Aedes aegypti en la transmisión, de los cuatro tipos conocidos del virus del Dengue y del virus de la Fiebre Amarilla Urbana. El Último episodio de Fiebre Amarilla Urbana en Colombia ocurrió en el año de 1929 en la población de Socorro (Santander; sin embargo, la presencia del mosquito en áreas urbanas cercanas a focos enzoóticos de Fiebre Amarilla Selvática constituye un peligro potencial. Desde el año de 1972 se vienen presentando en el país epidemias de Dengue debidas al virus tipo II, más tarde hizo su aparición el tipo III y más recientemente el tipoI, todos transmitidos por A. aegypti, el único vector conocido de Dengue en las Américas.

  18. Habitat productivity and pyrethroid susceptibility status of Aedes aegypti mosquitoes in Dar es Salaam, Tanzania.

    Science.gov (United States)

    Mathias, Leah; Baraka, Vito; Philbert, Anitha; Innocent, Ester; Francis, Filbert; Nkwengulila, Gamba; Kweka, Eliningaya J

    2017-06-09

    Aedes aegypti (Diptera: Culicidae) is the main vector of the dengue virus globally. Dengue vector control is mainly based on reducing the vector population through interventions, which target potential breeding sites. However, in Tanzania, little is known about this vector's habitat productivity and insecticide susceptibility status to support evidence-based implementation of control measures. The present study aimed at assessing the productivity and susceptibility status of A. aegypti mosquitoes to pyrethroid-based insecticides in Dar es Salaam, Tanzania. An entomological assessment was conducted between January and July 2015 in six randomly selected wards in Dar es Salaam, Tanzania. Habitat productivity was determined by the number of female adult A. aegypti mosquitoes emerged per square metre. The susceptibility status of adult A. aegypti females after exposure to 0.05% deltamethrin, 0.75% permethrin and 0.05% lambda-cyhalothrin was evaluated using the standard WHO protocols. Mortality rates were recorded after 24 h exposure and the knockdown effect was recorded at the time points of 10, 15, 20, 30, 40, 50 and 60 min to calculate the median knockdown times (KDT 50 and KDT 95 ). The results suggest that disposed tyres had the highest productivity, while water storage tanks had the lowest productivity among the breeding habitats Of A. aegypti mosquitoes. All sites demonstrated reduced susceptibility to deltamethrin (0.05%) within 24 h post exposure, with mortalities ranging from 86.3 ± 1.9 (mean ± SD) to 96.8 ± 0.9 (mean ± SD). The lowest and highest susceptibilities were recorded in Mikocheni and Sinza wards, respectively. Similarly, all sites demonstrated reduced susceptibility permethrin (0.75%) ranging from 83.1 ± 2.1% (mean ± SD) to 96.2 ± 0.9% (mean ± SD), in Kipawa and Sinza, respectively. Relatively low mortality rates were observed in relation to lambda-cyhalothrin (0.05%) at all sites, ranging from 83.1 ± 0

  19. The genetics of chemoreception in the labella and tarsi of Aedes aegypti.

    Science.gov (United States)

    Sparks, Jackson T; Bohbot, Jonathan D; Dickens, Joseph C

    2014-05-01

    The yellow-fever mosquito Aedes aegypti is a major vector of human diseases, such as dengue, yellow fever, chikungunya and West Nile viruses. Chemoreceptor organs on the labella and tarsi are involved in human host evaluation and thus serve as potential foci for the disruption of blood feeding behavior. In addition to host detection, these contact chemoreceptors mediate feeding, oviposition and conspecific recognition; however, the molecular landscape of chemoreception in these tissues remains mostly uncharacterized. Here we report the expression profile of all putative chemoreception genes in the labella and tarsi of both sexes of adult Ae. aegypti and discuss their possible roles in the physiology and behavior of this important disease vector. Published by Elsevier Ltd.

  20. Smile, you are on camera or in a live trap! The role of mammals in dispersion of jackfruit and native seeds in Ilha Grande State Park, Brazil

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    Daniel Santana Lorenzo Raíces

    2017-11-01

    Full Text Available The alien species Artocarpus heterophyllus, originally from India, was introduced to Brazil in the colonial period and has become invasive in some areas in the Atlantic Forest. Its fruits can weigh 35 kg and produce up to 500 seeds each. In their native range they are dispersed by turtles, rodents, monkeys, wild pigs and elephants. This study aimed to investigate the influence of mammals in jackfruit predation and seed dispersal, as well as the influence of jackfruit in native plant species dispersal by mammals, in the Ilha Grande State Park, Southeastern Brazil. Seeds with and without mesocarp were tied to thread spools and checked for predation and dispersion on 37 sites. We recorded mesocarp or jackfruit seed feeding on video. Six species of mammals were recorded feeding on jackfruit, but Trinomys dimidiatus, Didelphis aurita and Cuniculus paca accounted for 92% of all records. Cuniculus paca and Trinomys dimidiatus preyed and dispersed seeds while Didelphis aurita consumed mesocarp only. Seeds with mesocarp were more preyed on than seeds without mesocarp and its consumption was lower during more intense fruit production. Hence, jackfruit production can exceed the capacity of mammals to consume its seeds in areas where jackfruit density is high. Faeces of small mammals were collected in areas with (10 grids and without jackfruits (8 grids and analysed for the presence of native seeds. Twelve small mammal species were captured in areas with and without jackfruits, but faeces of 11 species were collected. Didelphis aurita dispersed proportionally more native seeds in area without jackfruits. Our results showed that mammals are playing a negative role helping to disperse jackfruit trees, and this is occurring in different ways depending on mammal species.

  1. Spatial partition of artificial structures by fish at the surroundings of the conservation unit - Parque Estadual da Ilha Anchieta, SP, Brazil

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    Patricia Teresa Monteiro Cunningham

    2004-03-01

    Full Text Available The aim of this work was to study the spatial partition dynamics of fish at artificial structures. Holed structured concrete blocks were used to construct eight identical artificial structures and disposed between 3m-6m depths. Installation was made in two steps during 1996 (May/June and November/December and daily observations were carried out during 30 consecutive days SCUBA diving. The artificial reef areas were used in discriminated ways by the fish community and was most probably influenced by several factor, mainly biotic. The results of the Krustal-Wallis test led to the refutation of the hypothesis that the artificial structure spaces were shared and randomly used by fish.Este trabalho é parte integrante de um projeto maior realizado pelo Laboratório ECOPEX/IOUSP. Foi desenvolvido nos entornos do Parque Estadual da Ilha Anchieta, Ubatuba, litoral norte de São Paulo, com o objetivo de estudar a dinâmica de repartição espacial dos peixes em estruturas artificiais e de testar a hipótese "os peixes repartem e utilizam aleatoriamente o espaço das estruturas artificiais". Utilizando-se blocos de concreto vazados, foram construídas e colocadas entre 3m - 6m, oito estruturas artificias idênticas. A instalação foi feita em duas etapas durante o ano de 1996 (Maio/Junho e Novembro/Dezembro e as observações efetuadas diariamente durante 30 dias consecutivos usando equipamento de mergulho autônomo. A ictiofauna utilizou de forma diferenciada as áreas dos recifes artificiais, influenciada provavelmente por vários fatores, principalmente bióticos. Os resultados do teste de Kruskal-Wallis levaram a refutar a hipótese desse estudo.

  2. Factors associated with sexual initiation and condom use among adolescents on Santiago Island, Cape Verde, West Africa Fatores associados ao início da vida sexual e o uso de preservativo entre adolescentes da Ilha de Santiago, Cabo Verde, África Ocidental

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    Carlos Mendes Tavares

    2009-09-01

    Full Text Available The current study focuses on factors associated with sexual initiation and condom use among teenagers on Santiago Island, Cape Verde, according to gender. This was a representative, probabilistic sample of 13-to-17-year-olds (n = 768 attending public secondary schools on Santiago Island in 2007. Associations were tested by test of proportion, Pearson's chi-square, or Fisher's exact test and logistic regression. Factors related to sexual initiation among boys were: age over 14 years, Catholic religion, and alcohol consumption. For girls, the factors included: > 9 years of schooling and involvement in an affective-sexual relationship. Unlike other Sub-Saharan countries, this study showed a high prevalence of condom use during initial sexual activity. Adolescents are able to safely begin sexually active life if they have access to information, sex education, and other STD prevention and contraceptive methods. This study provides insights on the development of policies to reduce the vulnerability of the young population to STD/AIDS and the limits and challenges related to the promotion of condom use and sex education, focusing on unequal gender relations.Foram analisados fatores associados ao início da vida sexual de adolescentes na Ilha de Santiago, Cabo Verde, segundo sexo. Estudo realizado com amostra probabilística e representativa de 768 adolescentes, age 13-17 anos, de escolas secundárias públicas da Ilha de Santiago em 2007. A associação foi testada pelo teste de proporção, qui-quadrado de Pearson ou Fisher e regressão logística. Nos rapazes, os fatores associados ao início da vida sexual foram: idade maior que 14 anos, ser católico e consumo de bebidas alcoólicas. Para meninas: escolaridade maior que nove anos e ter parceiro afetivo-sexual. Ao contrário de outros contextos da África Subsaariana, foram constatadas taxas elevadas de uso de preservativo por adolescentes no início da vida sexual. Os adolescentes podem iniciar a

  3. The Aquaporin Gene Family of the Yellow Fever Mosquito, Aedes aegypti

    OpenAIRE

    Drake, Lisa L.; Boudko, Dmitri Y.; Marinotti, Osvaldo; Carpenter, Victoria K.; Dawe, Angus L.; Hansen, Immo A.

    2010-01-01

    Background The mosquito, Aedes aegypti, is the principal vector of the Dengue and yellow fever viruses. During feeding, an adult female can take up more than its own body weight in vertebrate blood. After a blood meal females excrete large amounts of urine through their excretion system, the Malpighian tubules (MT). Diuresis starts within seconds after the mosquito starts feeding. Aquaporins (AQPs) are a family of membrane transporters that regulate the flow of water, glycerol and other small...

  4. Aedes aegypti entomological indices in an endemic area for dengue in Sao Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Eliane A Favaro

    2013-06-01

    Full Text Available OBJECTIVE: To evaluate the most productive types of properties and containers for Aedes aegypti and the spatial distribution of entomological indices. METHODS: Between December 2006 and February 2007, the vector's immature forms were collected to obtain entomological indices in 9,875 properties in the Jaguare neighborhood of Sao Jose do Rio Preto, SP, Southeastern Brazil. In March and April 2007, a questionnaire about the conditions and characteristics of properties was administered. Logistic regression was used to identify variables associated with the presence of pupae at the properties. Indices calculated per block were combined with a geo-referenced map, and thematic maps of these indices were obtained using statistical interpolation. RESULTS: The properties inspected had the following Ae. aegypti indices: Breteau Index = 18.9, 3.7 larvae and 0.42 pupae per property, 5.2 containers harboring Ae. aegypti per hectare, 100.0 larvae and 11.6 pupae per hectare, and 1.3 larvae and 0.15 pupae per inhabitant. The presence of yards, gardens and animals was associated with the presence of pupae. CONCLUSIONS: Specific types of properties and containers that simultaneously had low frequencies among those positive for the vector and high participation in the productivity of larvae and pupae were not identified. The use of indices including larval and pupal counts does not provide further information beyond that obtained from the traditional Stegomyia indices in locations with characteristics similar to those of São José do Rio Preto. The indices calculated per area were found to be more accurate for the spatial assessment of infestation. The Ae. aegypti infestation levels exhibited extensive spatial variation, indicating that the assessment of infestation in micro areas is needed.

  5. Dual African origins of global Aedes aegypti s.l. populations revealed by mitochondrial DNA.

    Directory of Open Access Journals (Sweden)

    Michelle Moore

    Full Text Available Aedes aegypti is the primary global vector to humans of yellow fever and dengue flaviviruses. Over the past 50 years, many population genetic studies have documented large genetic differences among global populations of this species. These studies initially used morphological polymorphisms, followed later by allozymes, and most recently various molecular genetic markers including microsatellites and mitochondrial markers. In particular, since 2000, fourteen publications and four unpublished datasets have used sequence data from the NADH dehydrogenase subunit 4 mitochondrial gene to compare Ae. aegypti collections and collectively 95 unique mtDNA haplotypes have been found. Phylogenetic analyses in these many studies consistently resolved two clades but no comprehensive study of mtDNA haplotypes have been made in Africa, the continent in which the species originated.ND4 haplotypes were sequenced in 426 Ae. aegypti s.l. from Senegal, West Africa and Kenya, East Africa. In Senegal 15 and in Kenya 7 new haplotypes were discovered. When added to the 95 published haplotypes and including 6 African Aedes species as outgroups, phylogenetic analyses showed that all but one Senegal haplotype occurred in a basal clade while most East African haplotypes occurred in a second clade arising from the basal clade. Globally distributed haplotypes occurred in both clades demonstrating that populations outside Africa consist of mixtures of mosquitoes from both clades.Populations of Ae. aegypti outside Africa consist of mosquitoes arising from one of two ancestral clades. One clade is basal and primarily associated with West Africa while the second arises from the first and contains primarily mosquitoes from East Africa.

  6. Family level variation in Wolbachia-mediated dengue virus blocking in Aedes aegypti

    OpenAIRE

    Terradas, Gerard; Allen, Scott L.; Chenoweth, Stephen F.; McGraw, Elizabeth A.

    2017-01-01

    Background The mosquito vector Aedes aegypti is responsible for transmitting a range of arboviruses including dengue (DENV) and Zika (ZIKV). The global reach of these viruses is increasing due to an expansion of the mosquito’s geographic range and increasing urbanization and human travel. Vector control remains the primary means for limiting these diseases. Wolbachia pipientis is an endosymbiotic bacterium of insects that has the ability to block the replication of pathogens, including flaviv...

  7. Larvicidal activity against Aedes aegypti of Foeniculum vulgare essential oils from Portugal and Cape Verde.

    Science.gov (United States)

    Rocha, Diara Kady; Matosc, Olivia; Novoa, Maria Teresa; Figueiredo, Ana Cristina; Delgado, Manuel; Moiteiro, Cristina

    2015-04-01

    Dengue is a potentially fatal mosquito-borne infection with 50 million cases per year and 2.5 billion people vulnerable to the disease. This major public health problem has recurrent epidemics in Latin America and occurred recently in Cape Verde and Madeira Island. The lack of anti-viral treatment or vaccine makes the control of mosquito vectors a high option to prevent virus transmission. Essential oil (EO) constituents can affect insect's behaviour, being potentially effective in pest control. The present study evaluated the potential use of Foenicultm vulgare (fennel) EO in the control of the dengue vector Aedes aegypti. EOs isolated from fennel aerial parts collected in Cape Verde and from a commercial fennel EO of Portugal were analysed by NMR, GC and GC-MS. trans-Anethole (32 and 30%, respectively), limonene (28 and 18%, respectively) and fenchone (10% in both cases) were the main compounds identified in the EOs isolated from fennel from Cape Verde and Portugal, respectively. The larvicidal activity of the EOs and its major constituents were evaluated, using WHO procedures, against third instar larvae ofAe. aegypti for 24 h. Pure compounds, such as limonene isomers, were also assayed. The lethal concentrations LC50, C90 and LC99 were determined by probit analysis using mortality rates of bioassays. A 99% mortality of Ae. aegypti larvae was estimated at 37.1 and 52.4 µL L-1 of fennel EOs from Cape Verde and Portugal, respectively. Bioassays showed that fennel EOs from both countries displayed strong larvicidal effect against Ae. aegypti, the Cape Verde EO being as active as one of its major constituents, (-)-limonene.

  8. Oviposition Site Selection by the Dengue Vector Aedes aegypti and Its Implications for Dengue Control

    Science.gov (United States)

    Wong, Jacklyn; Stoddard, Steven T.; Astete, Helvio; Morrison, Amy C.; Scott, Thomas W.

    2011-01-01

    Background Because no dengue vaccine or antiviral therapy is commercially available, controlling the primary mosquito vector, Aedes aegypti, is currently the only means to prevent dengue outbreaks. Traditional models of Ae. aegypti assume that population dynamics are regulated by density-dependent larval competition for food and little affected by oviposition behavior. Due to direct impacts on offspring survival and development, however, mosquito choice in oviposition site can have important consequences for population regulation that should be taken into account when designing vector control programs. Methodology/Principal Findings We examined oviposition patterns by Ae. aegypti among 591 naturally occurring containers and a set of experimental containers in Iquitos, Peru. Using larval starvation bioassays as an indirect measure of container food content, we assessed whether females select containers with the most food for their offspring. Our data indicate that choice of egg-laying site is influenced by conspecific larvae and pupae, container fill method, container size, lid, and sun exposure. Although larval food positively influenced oviposition, our results did not support the hypothesis that females act primarily to maximize food for larvae. Females were most strongly attracted to sites containing immature conspecifics, even when potential competitors for their progeny were present in abundance. Conclusion/Significance Due to strong conspecific attraction, egg-laying behavior may contribute more to regulating Ae. aegypti populations than previously thought. If highly infested containers are targeted for removal or larvicide application, females that would have preferentially oviposited in those sites may instead distribute their eggs among other suitable, previously unoccupied containers. Strategies that kill mosquitoes late in their development (i.e., insect growth regulators that kill pupae rather than larvae) will enhance vector control by creating

  9. Rotenoids from Tephrosia toxicaria with larvicidal activity against Aedes aegypti, the main vector of dengue fever

    International Nuclear Information System (INIS)

    Vasconcelos, Jackson Nunes e; Santiago, Gilvandete Maria Pinheiro; Lima, Jefferson Queiroz; Arriaga, Angela Martha Campos

    2012-01-01

    In the search for new larvicides from plants, we have investigated the potential activity of the rotenoids deguelin (1), 12a-hydroxy-a-toxicarol (2) and tephrosin (3), isolated from the bioactive ethanol extract of roots of Tephrosia toxicaria Pers., against Aedes aegypti, the main vector of dengue. The absolute configuration of these compounds was determined by circular dichroism (CD) spectra. The LC50 values of the compounds evaluated justify the potential of T. toxicaria as a new natural larvicide. (author)

  10. Evaluation of Aedes Aegypti Presence and Abundance in Septic Tanks and Their Impacts on Dengue Transmission

    Science.gov (United States)

    2009-03-10

    aforementioned applications, GIS has been used for cost analysis decisions in vector control planning . GIS software was used to remotely identify... plan for dengue outbreaks, a contingency plan to hospitalize large numbers of dengue hemorrhagic patients if necessary, education of the medical...de nicho en tres culicidae urbanos (Culex fatigans Weid, C. coringer Theo, y Aedes aegypti L.) en el cemeterio de Caracas. Acta Cientifica

  11. Synergistic Efficacy of Aedes aegypti Antimicrobial Peptide Cecropin A2 and Tetracycline against Pseudomonas aeruginosa

    OpenAIRE

    Zheng, Zhaojun; Tharmalingam, Nagendran; Liu, Qingzhong; Jayamani, Elamparithi; Kim, Wooseong; Fuchs, Beth Burgwyn; Zhang, Rijun; Vilcinskas, Andreas; Mylonakis, Eleftherios

    2017-01-01

    The increasing prevalence of antibiotic resistance has created an urgent need for alternative drugs with new mechanisms of action. Antimicrobial peptides (AMPs) are promising candidates that could address the spread of multidrug-resistant bacteria, either alone or in combination with conventional antibiotics. We studied the antimicrobial efficacy and bactericidal mechanism of cecropin A2, a 36-residue α-helical cationic peptide derived from Aedes aegypti cecropin A, focusing on the common pat...

  12. Treatment of horses with cypermethrin against the biting flies Culicoides nubeculosus, Aedes aegypti and Culex quinquefasciatus.

    Science.gov (United States)

    Papadopoulos, E; Rowlinson, M; Bartram, D; Carpenter, S; Mellor, P; Wall, R

    2010-04-19

    An in vitro assay was used to assess the efficacy of the proprietary pyrethroid insecticide cypermethrin applied to horses (Deosect spray, 5.0%, w/v Fort Dodge Animal Health) against the biting midge Culicoides nubeculosus (Meigen) (Diptera: Ceratopogonidae) and the mosquitoes Aedes aegypti Linneaus and Culex quinquefasciatus Say (Diptera: Culicidae). Hair was collected from the back, belly and legs of the horses immediately prior to treatment and 7, 14, 21, 28 and 35 days after treatment, and also from untreated controls. In laboratory assays groups of 10 adult female C. nubeculosus, Ae. aegypti or C. quinquefasciatus were exposed to 0.5g of hair for 3min. In all cases, little or no mortality was observed in insects kept in contact with the pre-treatment samples or the untreated controls. With post-treatment samples for C. nubeculosus, mortality was close to 80% 7 days after treatment and then declined gradually; mean mortality was still at around 50% for hair collected 35 days after treatment. In general, Ae. aegypti and C. quinquefasciatus appeared to be less susceptible to cypermethrin than C. nubeculosus and the attenuation of the toxic effect declined more quickly with time after treatment. There were differences in the toxicity of hair from different body regions, with hair from the back consistently inducing the highest mortality and hair from the legs the lowest; this effect was more pronounced for C. nubeculosus than Ae. aegypti or C. quinquefasciatus. The results demonstrate the potential for topical insecticide treatment to offer protection to horses against biting flies; but highlight the major differences that exist in susceptibility between different insect species.

  13. Determinants of Heterogeneous Blood Feeding Patterns by Aedes aegypti in Iquitos, Peru

    OpenAIRE

    Liebman, Kelly A.; Stoddard, Steven T.; Reiner, Robert C.; Perkins, T. Alex; Astete, Helvio; Sihuincha, Moises; Halsey, Eric S.; Kochel, Tadeusz J.; Morrison, Amy C.; Scott, Thomas W.

    2014-01-01

    Background Heterogeneous mosquito biting results in different individuals in a population receiving an uneven number of bites. This is a feature of many vector-borne disease systems that, if understood, could guide preventative control efforts toward individuals who are expected to contribute most to pathogen transmission. We aimed to characterize factors determining biting patterns of Aedes aegypti, the principal mosquito vector of dengue virus. Methodology/Principal Findings Engorged female...

  14. Wolbachia Infection Reduces Blood-Feeding Success in the Dengue Fever Mosquito, Aedes aegypti

    OpenAIRE

    Turley, Andrew P.; Moreira, Luciano A.; O'Neill, Scott L.; McGraw, Elizabeth A.

    2009-01-01

    BACKGROUND: The mosquito Aedes aegypti was recently transinfected with a life-shortening strain of the endosymbiont Wolbachia pipientis (wMelPop) as the first step in developing a biocontrol strategy for dengue virus transmission. In addition to life-shortening, the wMelPop-infected mosquitoes also exhibit increased daytime activity and metabolic rates. Here we sought to quantify the blood-feeding behaviour of Wolbachia-infected females as an indicator of any virulence or energetic drain asso...

  15. Application of wMelPop Wolbachia Strain to Crash Local Populations of Aedes aegypti.

    Directory of Open Access Journals (Sweden)

    Scott A Ritchie

    Full Text Available The endosymbiotic bacteria Wolbachia pipientis (wMel strain has been successfully established in several populations of Aedes aegypti, the primary dengue vector. The virulent Wolbachia strain wMelPop is known to cause several pathological impacts (increased egg mortality, life shortening, etc. reducing overall fitness in the mosquito Ae. aegypti. Increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populations. We tested this application under semi-field cage conditions. First, we determined that wMelPop infection significantly reduced the survival of desiccation-resistant eggs of the dengue vector Ae. aegypti, with shade and temperature having a significant impact; nearly all wMelPop-infected eggs failed to hatch after 6 and 10 weeks in summer and winter conditions, respectively. In laboratory selection experiments we found that egg desiccation resistance can be increased by selection, and that this effect of wMelPop infection is due to the nuclear background of the host rather than Wolbachia. We then conducted an invasion of wMelPop within a semi-field cage using sustained weekly releases of wMelPop infected mosquitoes, with fixation achieved after 9 weeks. The egg populations wMelPop infected and an uninfected control were then subjected to a simulated prolonged monsoonal dry season (2.5 months before flooding to induce hatching. The wMelPop infected eggs suffered significantly greater mortality than the controls, with only 0.67% and 4.35% of respective infected and uninfected eggs held in 99% shade hatching after 80 days. These studies suggest that wMelPop could be used to locally eliminate populations of Ae. aegypti that are exposed to prolonged dry conditions, particularly if combined with vector control.

  16. Toxicity of spinosad to temephos-resistant Aedes aegypti populations in Brazil

    Science.gov (United States)

    dos Santos Dias, Luciana; Macoris, Maria de Lourdes da Graça; Andrighetti, Maria Teresa Macoris; Otrera, Vanessa Camargo Garbeloto; Dias, Adriana dos Santos; Bauzer, Luiz Guilherme Soares da Rocha; Rodovalho, Cynara de Melo; Martins, Ademir Jesus; Lima, José Bento Pereira

    2017-01-01

    The mosquito Aedes aegypti is the primary vector of different arboviruses and represents a major public health problem. Several Brazilian populations of Ae. aegypti have developed resistance to temephos, the most used organophosphate larvicide. New tools which are less harmful to the environment and safer for humans are becoming increasingly important to control this insect vector. Spinosad, an aerobic fermentation product of a soil actinobacteria, has a favorable environmental profile. It presents selective insecticide properties, a mechanism of action that differs from those of many synthetic chemical insecticides. The toxicity of spinosad and temephos to Aedes aegypti populations from Brazil, which were previously exposed to temephos, were investigated in this study. Larval susceptibility (LC50) to temephos varied from 3μg/L for Rockefeller up to 260 μg/L for Santana do Ipanema field derived population. Larval susceptibility (LC50) to spinosad varied from 23μg/L for Rockefeller up to 93μg/L for Marilia field derived population. In addition, a semi-field trial was performed to evaluate spinosad (NatularTM DT) initial efficacy and persistence toward four field-derived lineages and the Rockefeller lineage, used as an internal control. Spinosad was tested at 0.5mg active ingredient/L in 200L capacity water tanks. Mortality was recorded each 24 hours after exposition and tanks were further recolonized once per week with mortality being recorded daily for eight weeks. Spinosad provided a level equal or superior to 80% mortality during a seven to eight week evaluation period. The assessed populations did not present cross-resistance between spinosad and temephos in laboratory conditions. It demonstrates that spinosad may be a promising larvicide for the control of Ae. aegypti, especially for populations in which resistance to temephos has been detected. PMID:28301568

  17. Synthesis and Larvicidal and Adult Topical Activity of Some Hydrazide-Hydrazone Derivatives Against Aedes aegypti

    Science.gov (United States)

    2014-01-01

    anticancer, antifungal, antiviral, antitumoral, antibacterial and antimalarial activities [11-13]. Recently, our group has been investigating the...ABSTRACT A series of novel hydrazide-hydrazone derivatives were synthesized and evaluated for their larvicidal and adult topical activity against...4b) showed noteworthy larvacidal activity against Aedes aegypti. Dose-response data of compound 4b showed LC50 and LC90 values of 30.5 (15.4 – 22.7

  18. Active metabolites of the genus Piper against Aedes aegypti: natural alternative sources for dengue vector control

    OpenAIRE

    Marques, André M; Kaplan, Maria Auxiliadora C

    2015-01-01

    The mosquito, Aedes aegypti, is the principal vector of the viruses responsible for dengue and dengue hemorrhagic fevers. The mosquito is widespread throughout tropical and sub-tropical regions; its prevalence makes dengue one of the most important mosquito-borne viral diseases in the world occurring annually in more than 100 endemic countries. Because blood is essential to their development cycle, the Aedes species maintains a close association with humans and their dwellings. Fittingly, the...

  19. Insecticide resistance in two Aedes aegypti (Diptera: Culicidae) strains from Costa Rica.

    Science.gov (United States)

    Bisset, J A; Marín, R; Rodríguez, M M; Severson, D W; Ricardo, Y; French, L; Díaz, M; Pérez, O

    2013-03-01

    Dengue (family Flaviridae, genus Flavivirus, DENV) and dengue hemorrhagic fever (DHF) are presently important public health problems in Costa Rica. The primary strategy for disease control is based on reducing population densities of the main mosquito vector Aedes aegypti (L.) (Diptera: Culicidae). This is heavily dependent on use of chemical insecticides, thus the development of resistance is a frequent threat to control program effectiveness. The objective of this study was to determine the levels of insecticide resistance and the metabolic resistance mechanisms involved in two Ae. aegypti strains collected from two provinces (Puntarenas and Limon) in Costa Rica. Bioassays with larvae were performed according to World Health Organization guidelines and resistance in adults was measured through standard bottle assays. The activities of beta-esterases, cytochrome P450 monooxygenases, and glutathione S-transferases (GST), were assayed through synergists and biochemical tests, wherein the threshold criteria for each enzyme was established using the susceptible Rockefeller strain. The results showed higher resistance levels to the organophosphate (OP) temephos and the pyrethroid deltamethrin in larvae. The efficacy of commercial formulations of temephos in controlling Ae. aegypti populations was 100% mortality up to 11 and 12 d posttreatment with daily water replacements in test containers. Temephos and deltamethrin resistance in larvae were associated with high esterase activity, but not to cytochrome P450 monooxygenase or GST activities. Adult mosquitoes were resistant to deltamethrin, and susceptible to bendiocarb, chlorpyrifos, and cypermethrin. Because temephos and deltamethrin resistance are emerging at the studied sites, alternative insecticides should be considered. The insecticides chlorpyrifos and cypermethrin could be good candidates to use as alternatives for Ae. aegypti control.

  20. Reducing biting rates of Aedes aegypti with metofluthrin: investigations in time and space

    OpenAIRE

    Darbro, Jonathan M.; Muzari, M. Odwell; Giblin, Arthur; Adamczyk, Rebecca M.; Ritchie, Scott A.; Devine, Gregor J.

    2017-01-01

    Background Indoor residual spraying is key to dengue control in Cairns and other parts of northern Queensland, Australia, where Aedes aegypti is prevalent, but the strategy faces challenges with regards to slow application time and, therefore, community coverage. A faster potential improvement might be the use of polyethylene netting impregnated with the volatile pyrethroid metofluthrin (SumiOne?). This formulation was assessed in rooms in three houses in Cairns, Australia. One emanator was p...

  1. Larvicidal activity of Annona senegalensis and Boswellia dalzielii leaf fractions against Aedes aegypti (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Younoussa Lame

    2014-12-01

    Full Text Available The purpose of the present study was to evaluate the larvicidal activity of leaf fractions of Annona senegalensis and Boswellia dalzielii against fourth instar larvae of Aedes aegypti. Fourth instar larvae of Ae. aegypti were exposed for 24 hours to various concentrations (312.5-2500 mg/L of methanolic crude extract and its fractions obtained with n-hexane, chloroform, ethyl-acetate and methanol solvents, following WHO method. The mortalities recorded were subjected to ANOVA test for mean comparison and Probit analysis to determine LC50. Preliminary phytochemical screening test for some components of the plants assessed were also evaluated. The phytochemical screening of the two plants revealed the presence of alkaloids, steroids, phenolic compounds, terpenoids, fats and oils in the crude extracts which, after splitting were most distributed in n-hexane and chloroform fractions. Apart from methanol fraction, all products used showed a significant (P<0.001 concentration-dependent toxicity against Ae. aegypti larvae. The LC50 recorded with crude extract were 759.6 and 830.4 mg/L for A. senegalensis and B. dalzielli respectively. After fractionation, n-hexane and chloroform fractions of A. senegalensis revealed more effective activity than others with CL50 values of 379.3 and 595.2 mg/L respectively. As for B. dalzielli, n-hexane (LC50=537.1 mg/L and chloroform (LC50=585.5 mg/L fractions were also the most effective. These results suggest that the n-hexane and chloroform fractions of these plants as a promising larvicide against Ae. aegypti and can constitute the best basic and vital step in the development of a botanical insecticide source.

  2. Toxicity of spinosad to temephos-resistant Aedes aegypti populations in Brazil.

    Directory of Open Access Journals (Sweden)

    Luciana Dos Santos Dias

    Full Text Available The mosquito Aedes aegypti is the primary vector of different arboviruses and represents a major public health problem. Several Brazilian populations of Ae. aegypti have developed resistance to temephos, the most used organophosphate larvicide. New tools which are less harmful to the environment and safer for humans are becoming increasingly important to control this insect vector. Spinosad, an aerobic fermentation product of a soil actinobacteria, has a favorable environmental profile. It presents selective insecticide properties, a mechanism of action that differs from those of many synthetic chemical insecticides. The toxicity of spinosad and temephos to Aedes aegypti populations from Brazil, which were previously exposed to temephos, were investigated in this study. Larval susceptibility (LC50 to temephos varied from 3μg/L for Rockefeller up to 260 μg/L for Santana do Ipanema field derived population. Larval susceptibility (LC50 to spinosad varied from 23μg/L for Rockefeller up to 93μg/L for Marilia field derived population. In addition, a semi-field trial was performed to evaluate spinosad (NatularTM DT initial efficacy and persistence toward four field-derived lineages and the Rockefeller lineage, used as an internal control. Spinosad was tested at 0.5mg active ingredient/L in 200L capacity water tanks. Mortality was recorded each 24 hours after exposition and tanks were further recolonized once per week with mortality being recorded daily for eight weeks. Spinosad provided a level equal or superior to 80% mortality during a seven to eight week evaluation period. The assessed populations did not present cross-resistance between spinosad and temephos in laboratory conditions. It demonstrates that spinosad may be a promising larvicide for the control of Ae. aegypti, especially for populations in which resistance to temephos has been detected.

  3. How does competition among wild type mosquitoes influence the performance of Aedes aegypti and dissemination of Wolbachia pipientis?

    Directory of Open Access Journals (Sweden)

    Suellen de Oliveira

    2017-10-01

    Full Text Available Wolbachia has been deployed in several countries to reduce transmission of dengue, Zika and chikungunya viruses. During releases, Wolbachia-infected females are likely to lay their eggs in local available breeding sites, which might already be colonized by local Aedes sp. mosquitoes. Therefore, there is an urgent need to estimate the deleterious effects of intra and interspecific larval competition on mosquito life history traits, especially on the duration of larval development time, larval mortality and adult size.Three different mosquito populations were used: Ae. aegypti infected with Wolbachia (wMelBr strain, wild Ae. aegypti and wild Ae. albopictus. A total of 21 treatments explored intra and interspecific larval competition with varying larval densities, species proportions and food levels. Each treatment had eight replicates with two distinct food levels: 0.25 or 0.50 g of Chitosan and fallen avocado leaves. Overall, overcrowding reduced fitness correlates of the three populations. Ae. albopictus larvae presented lower larval mortality, shorter development time to adult and smaller wing sizes than Ae. aegypti. The presence of Wolbachia had a slight positive effect on larval biology, since infected individuals had higher survivorship than uninfected Ae. aegypti larvae.In all treatments, Ae. albopictus outperformed both wild Ae. aegypti and the Wolbachia-infected group in larval competition, irrespective of larval density and the amount of food resources. The major force that can slow down Wolbachia invasion is the population density of wild mosquitoes. Given that Ae. aegypti currently dominates in Rio, in comparison with Ae. albopictus frequency, additional attention must be given to the population density of Ae. aegypti during releases to increase the l