WorldWideScience

Sample records for advanced biofuels production

  1. Systems-Level Synthetic Biology for Advanced Biofuel Production

    Energy Technology Data Exchange (ETDEWEB)

    Ruffing, Anne [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Jensen, Travis J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Strickland, Lucas Marshall [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Meserole, Stephen [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Tallant, David [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-03-01

    Cyanobacteria have been shown to be capable of producing a variety of advanced biofuels; however, product yields remain well below those necessary for large scale production. New genetic tools and high throughput metabolic engineering techniques are needed to optimize cyanobacterial metabolisms for enhanced biofuel production. Towards this goal, this project advances the development of a multiple promoter replacement technique for systems-level optimization of gene expression in a model cyanobacterial host: Synechococcus sp. PCC 7002. To realize this multiple-target approach, key capabilities were developed, including a high throughput detection method for advanced biofuels, enhanced transformation efficiency, and genetic tools for Synechococcus sp. PCC 7002. Moreover, several additional obstacles were identified for realization of this multiple promoter replacement technique. The techniques and tools developed in this project will help to enable future efforts in the advancement of cyanobacterial biofuels.

  2. Biofuels Fuels Technology Pathway Options for Advanced Drop-in Biofuels Production

    Energy Technology Data Exchange (ETDEWEB)

    Kevin L Kenney

    2011-09-01

    Advanced drop-in hydrocarbon biofuels require biofuel alternatives for refinery products other than gasoline. Candidate biofuels must have performance characteristics equivalent to conventional petroleum-based fuels. The technology pathways for biofuel alternatives also must be plausible, sustainable (e.g., positive energy balance, environmentally benign, etc.), and demonstrate a reasonable pathway to economic viability and end-user affordability. Viable biofuels technology pathways must address feedstock production and environmental issues through to the fuel or chemical end products. Potential end products include compatible replacement fuel products (e.g., gasoline, diesel, and JP8 and JP5 jet fuel) and other petroleum products or chemicals typically produced from a barrel of crude. Considering the complexity and technology diversity of a complete biofuels supply chain, no single entity or technology provider is capable of addressing in depth all aspects of any given pathway; however, all the necessary expert entities exist. As such, we propose the assembly of a team capable of conducting an in-depth technology pathway options analysis (including sustainability indicators and complete LCA) to identify and define the domestic biofuel pathways for a Green Fleet. This team is not only capable of conducting in-depth analyses on technology pathways, but collectively they are able to trouble shoot and/or engineer solutions that would give industrial technology providers the highest potential for success. Such a team would provide the greatest possible down-side protection for high-risk advanced drop-in biofuels procurement(s).

  3. Molecular Breeding of Advanced Microorganisms for Biofuel Production

    Directory of Open Access Journals (Sweden)

    Hiroshi Sakuragi

    2011-01-01

    Full Text Available Large amounts of fossil fuels are consumed every day in spite of increasing environmental problems. To preserve the environment and construct a sustainable society, the use of biofuels derived from different kinds of biomass is being practiced worldwide. Although bioethanol has been largely produced, it commonly requires food crops such as corn and sugar cane as substrates. To develop a sustainable energy supply, cellulosic biomass should be used for bioethanol production instead of grain biomass. For this purpose, cell surface engineering technology is a very promising method. In biobutanol and biodiesel production, engineered host fermentation has attracted much attention; however, this method has many limitations such as low productivity and low solvent tolerance of microorganisms. Despite these problems, biofuels such as bioethanol, biobutanol, and biodiesel are potential energy sources that can help establish a sustainable society.

  4. 75 FR 20085 - Subpart B-Advanced Biofuel Payment Program

    Science.gov (United States)

    2010-04-16

    ... Rural Business-Cooperative Service 7 CFR Part 4288 RIN 0570-AA75 Subpart B--Advanced Biofuel Payment... biofuels to support existing advanced biofuel production and to encourage new production of advanced biofuels. The Agency would enter into contracts with advanced biofuel producers to pay such producers...

  5. Production of advanced biofuels: Co-processing of upgraded pyrolysis oil in standard refinery units

    NARCIS (Netherlands)

    Miguel Mercader, de F.; Groeneveld, M.J.; Kersten, S.R.A.; Way, N.W.J.; Schaverien, C.J.; Hogendoorn, J.A.

    2010-01-01

    One of the possible process options for the production of advanced biofuels is the co-processing of upgraded pyrolysis oil in standard refineries. The applicability of hydrodeoxygenation (HDO) was studied as a pyrolysis oil upgrading step to allow FCC co-processing. Different HDO reaction end temper

  6. Process modeling and supply chain design for advanced biofuel production based on bio-oil gasification

    Science.gov (United States)

    Li, Qi

    As a potential substitute for petroleum-based fuel, second generation biofuels are playing an increasingly important role due to their economic, environmental, and social benefits. With the rapid development of biofuel industry, there has been an increasing literature on the techno-economic analysis and supply chain design for biofuel production based on a variety of production pathways. A recently proposed production pathway of advanced biofuel is to convert biomass to bio-oil at widely distributed small-scale fast pyrolysis plants, then gasify the bio-oil to syngas and upgrade the syngas to transportation fuels in centralized biorefinery. This thesis aims to investigate two types of assessments on this bio-oil gasification pathway: techno-economic analysis based on process modeling and literature data; supply chain design with a focus on optimal decisions for number of facilities to build, facility capacities and logistic decisions considering uncertainties. A detailed process modeling with corn stover as feedstock and liquid fuels as the final products is presented. Techno-economic analysis of the bio-oil gasification pathway is also discussed to assess the economic feasibility. Some preliminary results show a capital investment of 438 million dollar and minimum fuel selling price (MSP) of $5.6 per gallon of gasoline equivalent. The sensitivity analysis finds that MSP is most sensitive to internal rate of return (IRR), biomass feedstock cost, and fixed capital cost. A two-stage stochastic programming is formulated to solve the supply chain design problem considering uncertainties in biomass availability, technology advancement, and biofuel price. The first-stage makes the capital investment decisions including the locations and capacities of the decentralized fast pyrolysis plants and the centralized biorefinery while the second-stage determines the biomass and biofuel flows. The numerical results and case study illustrate that considering uncertainties can be

  7. Identification and microbial production of a terpene-based advanced biofuel.

    Science.gov (United States)

    Peralta-Yahya, Pamela P; Ouellet, Mario; Chan, Rossana; Mukhopadhyay, Aindrila; Keasling, Jay D; Lee, Taek Soon

    2011-01-01

    Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of biofuels. PMID:21952217

  8. Genetic Resources for Advanced Biofuel Production Described with the Gene Ontology

    Directory of Open Access Journals (Sweden)

    Trudy eTorto-Alalibo

    2014-10-01

    Full Text Available Dramatic increases in research in the area of microbial biofuel production coupled with high-throughput data generation on bioenergy-related microbes has led to a deluge of information in the scientific literature and in databases. Consolidating this information and making it easily accessible requires a unified vocabulary. The Gene Ontology (GO fulfills that requirement, as it is a well-developed structured vocabulary that describes the activities and locations of gene products in a consistent manner across all kingdoms of life. The Microbial Energy Gene Ontology (MENGO: http://www.mengo.biochem.vt.edu project is extending the GO to include new terms to describe microbial processes of interest to bioenergy production. Our effort has added over 600 bioenergy related terms to the Gene Ontology. These terms will aid in the comprehensive annotation of gene products from diverse energy-related microbial genomes. An area of microbial energy research that has received a lot of attention is microbial production of advanced biofuels. These include alcohols such as butanol, isopropanol, isobutanol, and fuels derived from fatty acids, isoprenoids, and polyhydroxyalkanoates. These fuels are superior to first generation biofuels (ethanol and biodiesel esterified from vegetable oil or animal fat, can be generated from non-food feedstock sources, can be used as supplements or substitutes for gasoline, diesel and jet fuels, and can be stored and distributed using existing infrastructure. Here we review the roles of genes associated with synthesis of advanced biofuels, and at the same time introduce the use of the GO to describe the functions of these genes in a standardized way.

  9. Conventional and advanced liquid biofuels

    Directory of Open Access Journals (Sweden)

    Đurišić-Mladenović Nataša L.

    2016-01-01

    Full Text Available Energy security and independence, increase and fluctuation of the oil price, fossil fuel resources depletion and global climate change are some of the greatest challanges facing societies today and in incoming decades. Sustainable economic and industrial growth of every country and the world in general requires safe and renewable resources of energy. It has been expected that re-arrangement of economies towards biofuels would mitigate at least partially problems arised from fossil fuel consumption and create more sustainable development. Of the renewable energy sources, bioenergy draws major and particular development endeavors, primarily due to the extensive availability of biomass, already-existence of biomass production technologies and infrastructure, and biomass being the sole feedstock for liquid fuels. The evolution of biofuels is classified into four generations (from 1st to 4th in accordance to the feedstock origin; if the technologies of feedstock processing are taken into account, than there are two classes of biofuels - conventional and advanced. The conventional biofuels, also known as the 1st generation biofuels, are those produced currently in large quantities using well known, commercially-practiced technologies. The major feedstocks for these biofuels are cereals or oleaginous plants, used also in the food or feed production. Thus, viability of the 1st generation biofuels is questionable due to the conflict with food supply and high feedstocks’ cost. This limitation favoured the search for non-edible biomass for the production of the advanced biofuels. In a general and comparative way, this paper discusses about various definitions of biomass, classification of biofuels, and brief overview of the biomass conversion routes to liquid biofuels depending on the main constituents of the biomass. Liquid biofuels covered by this paper are those compatible with existing infrastructure for gasoline and diesel and ready to be used in

  10. Biofuel production in Vietnam

    NARCIS (Netherlands)

    Thanh, le L.

    2016-01-01

    Biofuel production has continued to develop and is driven by government support around the world. A comprehensive analysis of biofuel production and the policy implementation is crucial for the biofuel sustainability development. The objective of this thesis is to study the energy efficiency, GHG em

  11. Advancing Commercialization of Algal Biofuels Through Increased Biomass Productivity and Technology Integration

    Energy Technology Data Exchange (ETDEWEB)

    Bai, Xuemei [Cellana LLC; Sabarsky, Martin

    2013-09-30

    Cellana is a leading developer of algae-based bioproducts, and its pre-commercial production of marine microalgae takes place at Cellana?s Kona Demonstration Facility (KDF) in Hawaii. KDF is housing more than 70 high-performing algal strains for different bioproducts, of which over 30 have been grown outside at scale. So far, Cellana has produced more than 10 metric tons of algal biomass for the development of biofuels, animal feed, and high-value nutraceuticals. Cellana?s ALDUO algal cultivation technology allows Cellana to grow non-extremophile algal strains at large scale with no contamination disruptions. Cellana?s research and production at KDF have addressed three major areas that are crucial for the commercialization of algal biofuels: yield improvement, cost reduction, and the overall economics. Commercially acceptable solutions have been developed and tested for major factors limiting areal productivity of algal biomass and lipids based on years of R&D work conducted at KDF. Improved biomass and lipid productivity were achieved through strain improvement, culture management strategies (e.g., alleviation of self-shading, de-oxygenation, and efficient CO2 delivery), and technical advancement in downstream harvesting technology. Cost reduction was achieved through optimized CO2 delivery system, flue gas utilization technology, and energy-efficient harvesting technology. Improved overall economics was achieved through a holistic approach by integration of high-value co-products in the process, in addition to yield improvements and cost reductions.

  12. Engineering the fatty acid metabolic pathway in Saccharomyces cerevisiae for advanced biofuel production

    Directory of Open Access Journals (Sweden)

    Xiaoling Tang

    2015-12-01

    Full Text Available Fatty acid-derived fuels and chemicals have attracted a great deal of attention in recent decades, due to their following properties of high compatibility to gasoline-based fuels and existing infrastructure for their direct utilization, storage and distribution. The yeast Saccharomyces cerevisiae is the ideal biofuel producing candidate, based on the wealth of available genetic information and versatile tools designed to manipulate its metabolic pathways. Engineering the fatty acid metabolic pathways in S. cerevisiae is an effective strategy to increase its fatty acid biosynthesis and provide more pathway precursors for production of targeted products. This review summarizes the recent progress in metabolic engineering of yeast cells for fatty acids and fatty acid derivatives production, including the regulation of acetyl-CoA biosynthesis, NADPH production, fatty acid elongation, and the accumulation of activated precursors of fatty acids for converting enzymes. By introducing specific enzymes in the engineered strains, a powerful platform with a scalable, controllable and economic route for advanced biofuel production has been established.

  13. 76 FR 24343 - Advanced Biofuel Payment Program; Correction

    Science.gov (United States)

    2011-05-02

    ... Service Rural Utilities Service 7 CFR Part 4288 RIN 0570-AA75 Advanced Biofuel Payment Program; Correction... Advanced Biofuel Payment Program authorized under the Food, Conservation, and Energy Act of 2008. This... contracts with advanced biofuel producers to pay such producers for the production of eligible...

  14. Synthetic Biology Guides Biofuel Production

    OpenAIRE

    2010-01-01

    The advancement of microbial processes for the production of renewable liquid fuels has increased with concerns about the current fuel economy. The development of advanced biofuels in particular has risen to address some of the shortcomings of ethanol. These advanced fuels have chemical properties similar to petroleum-based liquid fuels, thus removing the need for engine modification or infrastructure redesign. While the productivity and titers of each of these processes remains to be improve...

  15. Analysis of advanced biofuels.

    Energy Technology Data Exchange (ETDEWEB)

    Dec, John E.; Taatjes, Craig A.; Welz, Oliver; Yang, Yi

    2010-09-01

    Long chain alcohols possess major advantages over ethanol as bio-components for gasoline, including higher energy content, better engine compatibility, and less water solubility. Rapid developments in biofuel technology have made it possible to produce C{sub 4}-C{sub 5} alcohols efficiently. These higher alcohols could significantly expand the biofuel content and potentially replace ethanol in future gasoline mixtures. This study characterizes some fundamental properties of a C{sub 5} alcohol, isopentanol, as a fuel for homogeneous-charge compression-ignition (HCCI) engines. Wide ranges of engine speed, intake temperature, intake pressure, and equivalence ratio are investigated. The elementary autoignition reactions of isopentanol is investigated by analyzing product formation from laser-photolytic Cl-initiated isopentanol oxidation. Carbon-carbon bond-scission reactions in the low-temperature oxidation chemistry may provide an explanation for the intermediate-temperature heat release observed in the engine experiments. Overall, the results indicate that isopentanol has a good potential as a HCCI fuel, either in neat form or in blend with gasoline.

  16. Advances in biofuel production from oil palm and palm oil processing wastes: A review

    OpenAIRE

    Jundika C. Kurnia; Sachin V. Jangam; Saad Akhtar; Sasmito, Agus P.; Mujumdar, Arun S.

    2016-01-01

    Over the last decades, the palm oil industry has been growing rapidly due to increasing demands for food, cosmetic, and hygienic products. Aside from producing palm oil, the industry generates a huge quantity of residues (dry and wet) which can be processed to produce biofuel. Driven by the necessity to find an alternative and renewable energy/fuel resources, numerous technologies have been developed and more are being developed to process oil-palm and palm-oil wastes into biofuel. To further...

  17. Microalgae: biofuel production

    OpenAIRE

    Babita Kumari; Vinay Sharma

    2013-01-01

    In the present day, microalgae feedstocks are gaining interest in energy scenario due to their fast growth potential coupled with relatively high lipid, carbohydrate and nutrients contents. All of these properties render them an excellent source for biofuels such as biodiesel, bioethanol and biomethane; as well as a number of other valuable pharmaceutical and nutraceutical products. The present review is a critical appraisal of the commercialization potential of microalgae biofuels....

  18. Advances in biofuel production from oil palm and palm oil processing wastes: A review

    Directory of Open Access Journals (Sweden)

    Jundika C. Kurnia

    2016-03-01

    Full Text Available Over the last decades, the palm oil industry has been growing rapidly due to increasing demands for food, cosmetic, and hygienic products. Aside from producing palm oil, the industry generates a huge quantity of residues (dry and wet which can be processed to produce biofuel. Driven by the necessity to find an alternative and renewable energy/fuel resources, numerous technologies have been developed and more are being developed to process oil-palm and palm-oil wastes into biofuel. To further develop these technologies, it is essential to understand the current stage of the industry and technology developments. The objective of this paper is to provide an overview of the palm oil industry, review technologies available to process oil palm and palm oil residues into biofuel, and to summarise the challenges that should be overcome for further development. The paper also discusses the research and development needs, technoeconomics, and life cycle analysis of biofuel production from oil-palm and palm-oil wastes.

  19. Outlook for advanced biofuels

    NARCIS (Netherlands)

    Hamelinck, Carlo Noël

    2004-01-01

    Modern use of biomass can play an important role in a sustainable energy supply. Biomass abounds in most parts of the world and substantial amounts could be produced at low costs. Motor biofuels seem a sensible application of biomass: they are among the few sustainable alternatives to the tran

  20. Synthetic Biology Guides Biofuel Production

    Directory of Open Access Journals (Sweden)

    Michael R. Connor

    2010-01-01

    Full Text Available The advancement of microbial processes for the production of renewable liquid fuels has increased with concerns about the current fuel economy. The development of advanced biofuels in particular has risen to address some of the shortcomings of ethanol. These advanced fuels have chemical properties similar to petroleum-based liquid fuels, thus removing the need for engine modification or infrastructure redesign. While the productivity and titers of each of these processes remains to be improved, progress in synthetic biology has provided tools to guide the engineering of these processes through present and future challenges.

  1. Fermentative biofuels production

    International Nuclear Information System (INIS)

    The limited reserves and increasing prices of fossil carbohydrates, as well as the global warming due to their utilization, impose the finding of renewable energy sources. Because of this, since decades an increasing interest in production of alcohols, which can be used as a fuel additives or fuels for direct replacement in gasoline engines, is observed. Alcohols can be obtained chemically or as products of microbial metabolism of different species in fermentation of sugars or starchy materials. In the present review are summarized different fermentative pathways for production of all alcohols, which are or could be used as biofuels. The focus of the paper is on production limitations, strains development and economical perspectives. Key words: fermentation, biofuel, alcohols

  2. Global Economic Effects of USA Biofuel Policy and the Potential Contribution from Advanced Biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Gbadebo Oladosu; Keith Kline; Paul Leiby; Rocio Uria-Martinez; Maggie Davis; Mark Downing; Laurence Eaton

    2012-01-01

    This study evaluates the global economic effects of the USA renewable fuel standards (RFS2), and the potential contribution from advanced biofuels. Our simulation results imply that these mandates lead to an increase of 0.21 percent in the global gross domestic product (GDP) in 2022, including an increase of 0.8 percent in the USA and 0.02 percent in the rest of the world (ROW); relative to our baseline, no-RFS scenario. The incremental contributions to GDP from advanced biofuels in 2022 are estimated at 0.41 percent and 0.04 percent in the USA and ROW, respectively. Although production costs of advanced biofuels are higher than for conventional biofuels in our model, their economic benefits result from reductions in oil use, and their smaller impacts on food markets compared with conventional biofuels. Thus, the USA advanced biofuels targets are expected to have positive economic benefits.

  3. Microalgae: biofuel production

    Directory of Open Access Journals (Sweden)

    Babita Kumari

    2013-04-01

    Full Text Available In the present day, microalgae feedstocks are gaining interest in energy scenario due to their fast growth potential coupled with relatively high lipid, carbohydrate and nutrients contents. All of these properties render them an excellent source for biofuels such as biodiesel, bioethanol and biomethane; as well as a number of other valuable pharmaceutical and nutraceutical products. The present review is a critical appraisal of the commercialization potential of microalgae biofuels. The available literature on various aspects of microalgae for e.g. its cultivation, life cycle assessment, and conceptualization of an algal biorefinery, has been done. The evaluation of available information suggests the operational and maintenance cost along with maximization of oil-rich microalgae production is the key factor for successful commercialization of microalgae-based fuels.

  4. Identification and microbial production of a terpene-based advanced biofuel

    OpenAIRE

    Peralta-Yahya, Pamela P.; Ouellet, Mario; Chan, Rossana; Mukhopadhyay, Aindrila; Keasling, Jay D.; Lee, Taek Soon

    2011-01-01

    Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a c...

  5. Assessing the quality of a deliberative democracy mini-public event about advanced biofuel production and development in Canada.

    Science.gov (United States)

    Longstaff, Holly; Secko, David M

    2016-02-01

    The importance of evaluating deliberative public engagement events is well recognized, but such activities are rarely conducted for a variety of theoretical, political and practical reasons. In this article, we provide an assessment of the criteria presented in the 2008 National Research Council report on Public Participation in Environmental Assessment and Decision Making (NRC report) as explicit indicators of quality for the 2012 'Advanced Biofuels' deliberative democracy event. The National Research Council's criteria were selected to evaluate this event because they are decision oriented, are the products of an exhaustive review of similar past events, are intended specifically for environmental processes and encompass many of the criteria presented in other evaluation frameworks. It is our hope that the results of our study may encourage others to employ and assess the National Research Council's criteria as a generalizable benchmark that may justifiably be used in forthcoming deliberative events exploring different topics with different audiences. PMID:25164558

  6. Production of Advanced Biofuels via Liquefaction - Hydrothermal Liquefaction Reactor Design: April 5, 2013

    Energy Technology Data Exchange (ETDEWEB)

    Knorr, D.; Lukas, J.; Schoen, P.

    2013-11-01

    This report provides detailed reactor designs and capital costs, and operating cost estimates for the hydrothermal liquefaction reactor system, used for biomass-to-biofuels conversion, under development at Pacific Northwest National Laboratory. Five cases were developed and the costs associated with all cases ranged from $22 MM/year - $47 MM/year.

  7. 77 FR 5229 - Notice of Contract Proposals (NOCP) for Payments to Eligible Advanced Biofuel Producers

    Science.gov (United States)

    2012-02-02

    ... Payments to Eligible Advanced Biofuel Producers AGENCY: Rural Business-Cooperative Service and Rural... availability of up to $25 million to make payments to advanced biofuel producers for the production of eligible... participating in the Advanced Biofuel Payment Program for Fiscal Year 2012 were accepted from October 1,...

  8. Biofuels and biodiversity: principles for creating better policies for biofuel production.

    Science.gov (United States)

    Groom, Martha J; Gray, Elizabeth M; Townsend, Patricia A

    2008-06-01

    Biofuels are a new priority in efforts to reduce dependence on fossil fuels; nevertheless, the rapid increase in production of biofuel feedstock may threaten biodiversity. There are general principles that should be used in developing guidelines for certifying biodiversity-friendly biofuels. First, biofuel feedstocks should be grown with environmentally safe and biodiversity-friendly agricultural practices. The sustainability of any biofuel feedstock depends on good growing practices and sound environmental practices throughout the fuel-production life cycle. Second, the ecological footprint of a biofuel, in terms of the land area needed to grow sufficient quantities of the feedstock, should be minimized. The best alternatives appear to be fuels of the future, especially fuels derived from microalgae. Third, biofuels that can sequester carbon or that have a negative or zero carbon balance when viewed over the entire production life cycle should be given high priority. Corn-based ethanol is the worst among the alternatives that are available at present, although this is the biofuel that is most advanced for commercial production in the United States. We urge aggressive pursuit of alternatives to corn as a biofuel feedstock. Conservation biologists can significantly broaden and deepen efforts to develop sustainable fuels by playing active roles in pursuing research on biodiversity-friendly biofuel production practices and by helping define biodiversity-friendly biofuel certification standards.

  9. Biofuels and biodiversity: principles for creating better policies for biofuel production.

    Science.gov (United States)

    Groom, Martha J; Gray, Elizabeth M; Townsend, Patricia A

    2008-06-01

    Biofuels are a new priority in efforts to reduce dependence on fossil fuels; nevertheless, the rapid increase in production of biofuel feedstock may threaten biodiversity. There are general principles that should be used in developing guidelines for certifying biodiversity-friendly biofuels. First, biofuel feedstocks should be grown with environmentally safe and biodiversity-friendly agricultural practices. The sustainability of any biofuel feedstock depends on good growing practices and sound environmental practices throughout the fuel-production life cycle. Second, the ecological footprint of a biofuel, in terms of the land area needed to grow sufficient quantities of the feedstock, should be minimized. The best alternatives appear to be fuels of the future, especially fuels derived from microalgae. Third, biofuels that can sequester carbon or that have a negative or zero carbon balance when viewed over the entire production life cycle should be given high priority. Corn-based ethanol is the worst among the alternatives that are available at present, although this is the biofuel that is most advanced for commercial production in the United States. We urge aggressive pursuit of alternatives to corn as a biofuel feedstock. Conservation biologists can significantly broaden and deepen efforts to develop sustainable fuels by playing active roles in pursuing research on biodiversity-friendly biofuel production practices and by helping define biodiversity-friendly biofuel certification standards. PMID:18261147

  10. Center for Advanced Biofuel Systems (CABS) Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Kutchan, Toni M. [Donald Danforth Plant Science Center, St. Louis, MO (United States)

    2015-12-02

    One of the great challenges facing current and future generations is how to meet growing energy demands in an environmentally sustainable manner. Renewable energy sources, including wind, geothermal, solar, hydroelectric, and biofuel energy systems, are rapidly being developed as sustainable alternatives to fossil fuels. Biofuels are particularly attractive to the U.S., given its vast agricultural resources. The first generation of biofuel systems was based on fermentation of sugars to produce ethanol, typically from food crops. Subsequent generations of biofuel systems, including those included in the CABS project, will build upon the experiences learned from those early research results and will have improved production efficiencies, reduced environmental impacts and decreased reliance on food crops. Thermodynamic models predict that the next generations of biofuel systems will yield three- to five-fold more recoverable energy products. To address the technological challenges necessary to develop enhanced biofuel systems, greater understanding of the non-equilibrium processes involved in solar energy conversion and the channeling of reduced carbon into biofuel products must be developed. The objective of the proposed Center for Advanced Biofuel Systems (CABS) was to increase the thermodynamic and kinetic efficiency of select plant- and algal-based fuel production systems using rational metabolic engineering approaches grounded in modern systems biology. The overall strategy was to increase the efficiency of solar energy conversion into oils and other specialty biofuel components by channeling metabolic flux toward products using advanced catalysts and sensible design:1) employing novel protein catalysts that increase the thermodynamic and kinetic efficiencies of photosynthesis and oil biosynthesis; 2) engineering metabolic networks to enhance acetyl-CoA production and its channeling towards lipid synthesis; and 3) engineering new metabolic networks for the

  11. Hydrothermal treatment of oleaginous yeast for the recovery of free fatty acids for use in advanced biofuel production.

    Science.gov (United States)

    Espinosa-Gonzalez, Isabel; Parashar, Archana; Bressler, David C

    2014-10-10

    Microbial oils hold great potential as a suitable feedstock for the renewable production of biofuels. Specifically, the use of oleaginous yeasts offers several advantages related to cultivation and quality of lipid products. However, one of the major bottlenecks for large-scale production of yeast oils is found in the lipid extraction process. This work investigated the hydrothermal treatment of oleaginous yeast for hydrolysis and lipid extraction resulting in fatty acids used for biofuel production. The oleaginous yeast, Cryptococcus curvatus, was grown in 5 L bioreactors and the biomass slurry with 53±4% lipid content (dry weight basis) was treated at 280 °C for 1h with an initial pressure of 500 psi in batch stainless steel reactors. The hydrolysis product was separated and each of the resulting streams was further characterized. The hexane soluble fraction contained fatty acids from the hydrolysis of yeast triacylglycerides, and was low in nitrogen and minerals and could be directly integrated as feedstock into pyrolysis processing to produce biofuels. The proposed hydrothermal treatment addresses some current technological bottlenecks associated with traditional methodologies such as dewatering, oil extraction and co-product utilization. It also enhances the feasibility of using microbial biomass for production of renewable fuels and chemicals.

  12. From first generation biofuels to advanced solar biofuels.

    Science.gov (United States)

    Aro, Eva-Mari

    2016-01-01

    Roadmaps towards sustainable bioeconomy, including the production of biofuels, in many EU countries mostly rely on biomass use. However, although biomass is renewable, the efficiency of biomass production is too low to be able to fully replace the fossil fuels. The use of land for fuel production also introduces ethical problems in increasing the food price. Harvesting solar energy by the photosynthetic machinery of plants and autotrophic microorganisms is the basis for all biomass production. This paper describes current challenges and possibilities to sustainably increase the biomass production and highlights future technologies to further enhance biofuel production directly from sunlight. The biggest scientific breakthroughs are expected to rely on a new technology called "synthetic biology", which makes engineering of biological systems possible. It will enable direct conversion of solar energy to a fuel from inexhaustible raw materials: sun light, water and CO2. In the future, such solar biofuels are expected to be produced in engineered photosynthetic microorganisms or in completely synthetic living factories.

  13. From first generation biofuels to advanced solar biofuels.

    Science.gov (United States)

    Aro, Eva-Mari

    2016-01-01

    Roadmaps towards sustainable bioeconomy, including the production of biofuels, in many EU countries mostly rely on biomass use. However, although biomass is renewable, the efficiency of biomass production is too low to be able to fully replace the fossil fuels. The use of land for fuel production also introduces ethical problems in increasing the food price. Harvesting solar energy by the photosynthetic machinery of plants and autotrophic microorganisms is the basis for all biomass production. This paper describes current challenges and possibilities to sustainably increase the biomass production and highlights future technologies to further enhance biofuel production directly from sunlight. The biggest scientific breakthroughs are expected to rely on a new technology called "synthetic biology", which makes engineering of biological systems possible. It will enable direct conversion of solar energy to a fuel from inexhaustible raw materials: sun light, water and CO2. In the future, such solar biofuels are expected to be produced in engineered photosynthetic microorganisms or in completely synthetic living factories. PMID:26667057

  14. Advancing Biofuels: Balancing for Sustainability

    Science.gov (United States)

    As with most technologies, use of biofuels has both benefits and risks, which vary by feedstock. Expected benefits include increased energy independence, reduced consumption of fossil fuels, reduced emission of greenhouse gases and invigorated rural economies. Anticipated risks include potential com...

  15. Engineering industrial yeast for renewable advanced biofuels applications

    Science.gov (United States)

    The industrial yeast Saccharomyces cerevisiae is a candidate for the next-generation biocatalyst development due to its unique genomic background and robust performance in fermentation-based production. In order to meet challenges of renewable and sustainable advanced biofuels conversion including ...

  16. Cyanobacteria as a platform for biofuel production

    Directory of Open Access Journals (Sweden)

    Nicole E Nozzi

    2013-09-01

    Full Text Available Cyanobacteria have great potential as a platform for biofuel production because of their fast growth, ability to fix carbon dioxide gas, and their genetic tractability. Furthermore they do not require fermentable sugars or arable land for growth and so competition with cropland would be greatly reduced. In this perspective we discuss the challenges and areas for improvement most pertinent for advancing cyanobacterial fuel production, including: improving genetic parts, carbon fixation, metabolic flux, nutrient requirements on a large scale, and photosynthetic efficiency using natural light.

  17. Advanced Biofuels Processing Development Unit

    Data.gov (United States)

    Federal Laboratory Consortium — The ABPDU at LBNL has a unique mission to partner with industry, National Labs, Bioenergy Research Centers, and academia to optimize, integrate and scale production...

  18. Metabolomics of Clostridial Biofuel Production

    Energy Technology Data Exchange (ETDEWEB)

    Rabinowitz, Joshua D [Princeton Univ., NJ (United States); Aristilde, Ludmilla [Cornell Univ., Ithaca, NY (United States); Amador-Noguez, Daniel [Univ. of Wisconsin, Madison, WI (United States)

    2015-09-08

    Members of the genus Clostridium collectively have the ideal set of the metabolic capabilities for fermentative biofuel production: cellulose degradation, hydrogen production, and solvent excretion. No single organism, however, can effectively convert cellulose into biofuels. Here we developed, using metabolomics and isotope tracers, basic science knowledge of Clostridial metabolism of utility for future efforts to engineer such an organism. In glucose fermentation carried out by the biofuel producer Clostridium acetobutylicum, we observed a remarkably ordered series of metabolite concentration changes as the fermentation progressed from acidogenesis to solventogenesis. In general, high-energy compounds decreased while low-energy species increased during solventogenesis. These changes in metabolite concentrations were accompanied by large changes in intracellular metabolic fluxes, with pyruvate directed towards acetyl-CoA and solvents instead of oxaloacetate and amino acids. Thus, the solventogenic transition involves global remodeling of metabolism to redirect resources from biomass production into solvent production. In contrast to C. acetobutylicum, which is an avid fermenter, C. cellulolyticum metabolizes glucose only slowly. We find that glycolytic intermediate concentrations are radically different from fast fermenting organisms. Associated thermodynamic and isotope tracer analysis revealed that the full glycolytic pathway in C. cellulolyticum is reversible. This arises from changes in cofactor utilization for phosphofructokinase and an alternative pathway from phosphoenolpyruvate to pyruvate. The net effect is to increase the high-energy phosphate bond yield of glycolysis by 150% (from 2 to 5) at the expense of lower net flux. Thus, C. cellulolyticum prioritizes glycolytic energy efficiency over speed. Degradation of cellulose results in other sugars in addition to glucose. Simultaneous feeding of stable isotope-labeled glucose and unlabeled pentose sugars

  19. PRODUCTION OF BIOFUELS AND ITS IMPACT ON AGRICULTURE IN CROATIA

    Directory of Open Access Journals (Sweden)

    Tajana Krička

    2008-09-01

    Full Text Available There is a large potential for the production of energy crops on agricultural land. Global demand for food is expected to double within the coming 50 years, and demand for transportation fuels is expected to increase even more rapidly. There is a great need for renewable energy supplies for biofuel production that do not cause significant environmental harm and do not compete with food supply. In addition, biofuel by-products can be utilized as livestock feed with a substantial revenue source and significantly increases the profitability of the production process. Food-based biofuels can meet but a small portion of energy needs despite recent advances in crop yields and increased biofuel production efficiency. Therefore, biofuels that are non food-based are likely to be of far greater importance over the longer term. Reasonable values on the external effects are in most cases not enough to make agriculture-based biomass energy competitive so that considerable government subsidies are needed. Biofuels such as cellulosic ethanol that can be produced on agriculturally marginal lands with minimum fertilizer, pesticide, and fossil energy inputs, or produced with agricultural residues have potential to provide fuel supplies with greater environmental benefits that either petroleum or current food-based biofuels.

  20. Coupling of algal biofuel production with wastewater.

    Science.gov (United States)

    Bhatt, Neha Chamoli; Panwar, Amit; Bisht, Tara Singh; Tamta, Sushma

    2014-01-01

    Microalgae have gained enormous consideration from scientific community worldwide emerging as a viable feedstock for a renewable energy source virtually being carbon neutral, high lipid content, and comparatively more advantageous to other sources of biofuels. Although microalgae are seen as a valuable source in majority part of the world for production of biofuels and bioproducts, still they are unable to accomplish sustainable large-scale algal biofuel production. Wastewater has organic and inorganic supplements required for algal growth. The coupling of microalgae with wastewater is an effective way of waste remediation and a cost-effective microalgal biofuel production. In this review article, we will primarily discuss the possibilities and current scenario regarding coupling of microalgal cultivation with biofuel production emphasizing recent progress in this area.

  1. Systems analysis and futuristic designs of advanced biofuel factory concepts.

    Energy Technology Data Exchange (ETDEWEB)

    Chianelli, Russ; Leathers, James; Thoma, Steven George; Celina, Mathias Christopher; Gupta, Vipin P.

    2007-10-01

    The U.S. is addicted to petroleum--a dependency that periodically shocks the economy, compromises national security, and adversely affects the environment. If liquid fuels remain the main energy source for U.S. transportation for the foreseeable future, the system solution is the production of new liquid fuels that can directly displace diesel and gasoline. This study focuses on advanced concepts for biofuel factory production, describing three design concepts: biopetroleum, biodiesel, and higher alcohols. A general schematic is illustrated for each concept with technical description and analysis for each factory design. Looking beyond current biofuel pursuits by industry, this study explores unconventional feedstocks (e.g., extremophiles), out-of-favor reaction processes (e.g., radiation-induced catalytic cracking), and production of new fuel sources traditionally deemed undesirable (e.g., fusel oils). These concepts lay the foundation and path for future basic science and applied engineering to displace petroleum as a transportation energy source for good.

  2. Supercritical fluids technology for clean biofuel production

    Institute of Scientific and Technical Information of China (English)

    Dongsheng Wen; H.Jiang; Kai Zhang

    2009-01-01

    Biofuels are liquid or gaseous fuels that are predominantly produced from biomass for transport sector applications.As biofuels are renewable,sustainable,carbon neutral and environmentally benign,they have been proposed as promising alternative fuels for gasoline and diesel engines.This paper reviews state-of-the-art application of the supercritical fluid(SCF)technique in biofuels production that includes biodiesel from vegetable oils via the transesterification process,bio-hydrogen from the gasification and bio-oil from the lique-faction of biomass,with biodiesel production as the main focus. The global biofuel situation and biofuel economics are also reviewed.The SCF has been shown to be a promising technique for future large-scale biofuel production,especially for biodiesel production from waster oil and fat.Compared with conventional biofuel production methods,the SCF technology possesses a number of advantages that includes fast inetics,high fuel production rate,ease of continuous operation and elimination of the necessity of catalysts.The harsh operation environment,i.e. the high temperature and high pressure,and its request on the materials and associated cost are the main concerns for its wide application.

  3. Increasing Feedstock Production for Biofuels: Economic Drivers, Environmental Implications, and the Role of Research

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2009-10-27

    The Biomass Research and Development Board (Board) commissioned an economic analysis of feedstocks to produce biofuels. The Board seeks to inform investments in research and development needed to expand biofuel production. This analysis focuses on feedstocks; other interagency teams have projects underway for other parts of the biofuel sector (e.g., logistics). The analysis encompasses feedstocks for both conventional and advanced biofuels from agriculture and forestry sources.

  4. IEA Energy Technology Essentials: Biofuel Production

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-01-15

    The IEA Energy Technology Essentials series offers concise four-page updates on the different technologies for producing, transporting and using energy. Biofuel Production is the topic covered in this edition.

  5. Constructed wetlands as biofuel production systems

    Science.gov (United States)

    Liu, Dong; Wu, Xu; Chang, Jie; Gu, Baojing; Min, Yong; Ge, Ying; Shi, Yan; Xue, Hui; Peng, Changhui; Wu, Jianguo

    2012-03-01

    Clean biofuel production is an effective way to mitigate global climate change and energy crisis. Progress has been made in reducing greenhouse-gas (GHG) emissions and nitrogen fertilizer consumption through biofuel production. Here we advocate an alternative approach that efficiently produces cellulosic biofuel and greatly reduces GHG emissions using waste nitrogen through wastewater treatment with constructed wetlands in China. Our combined experimental and literature data demonstrate that the net life-cycle energy output of constructed wetlands is higher than that of corn, soybean, switchgrass, low-input high-diversity grassland and algae systems. Energy output from existing constructed wetlands is ~237% of the input for biofuel production and can be enhanced through optimizing the nitrogen supply, hydrologic flow patterns and plant species selection. Assuming that all waste nitrogen in China could be used by constructed wetlands, biofuel production can account for 6.7% of national gasoline consumption. We also find that constructed wetlands have a greater GHG reduction than the existing biofuel production systems in a full life-cycle analysis. This alternative approach is worth pursuing because of its great potential for straightforward operation, its economic competitiveness and many ecological benefits.

  6. Biomass and biofuels from microalgae advances in engineering and biology

    CERN Document Server

    Moheimani, Navid Reza; de Boer, Karne; Bahri, Parisa

    2015-01-01

    This comprehensive book details the most recent advances in the microalgae biological sciences and engineering technologies for biomass and biofuel production in order to meet the ongoing need for new and affordable sources of food, chemicals and energy for future generations. The chapters explore new microalgae cultivation techniques, including solid (biofilm) systems, and heterotrophic production methods, while also critically investigating topics such as combining wastewater as a source of nutrients, the effect of CO2 on growth, and converting biomass to methane through anaerobi

  7. Microalgae as sustainable renewable energy feedstock for biofuel production.

    Science.gov (United States)

    Medipally, Srikanth Reddy; Yusoff, Fatimah Md; Banerjee, Sanjoy; Shariff, M

    2015-01-01

    The world energy crisis and increased greenhouse gas emissions have driven the search for alternative and environmentally friendly renewable energy sources. According to life cycle analysis, microalgae biofuel is identified as one of the major renewable energy sources for sustainable development, with potential to replace the fossil-based fuels. Microalgae biofuel was devoid of the major drawbacks associated with oil crops and lignocelluloses-based biofuels. Algae-based biofuels are technically and economically viable and cost competitive, require no additional lands, require minimal water use, and mitigate atmospheric CO2. However, commercial production of microalgae biodiesel is still not feasible due to the low biomass concentration and costly downstream processes. The viability of microalgae biodiesel production can be achieved by designing advanced photobioreactors, developing low cost technologies for biomass harvesting, drying, and oil extraction. Commercial production can also be accomplished by improving the genetic engineering strategies to control environmental stress conditions and by engineering metabolic pathways for high lipid production. In addition, new emerging technologies such as algal-bacterial interactions for enhancement of microalgae growth and lipid production are also explored. This review focuses mainly on the problems encountered in the commercial production of microalgae biofuels and the possible techniques to overcome these difficulties. PMID:25874216

  8. Microalgae as Sustainable Renewable Energy Feedstock for Biofuel Production

    Directory of Open Access Journals (Sweden)

    Srikanth Reddy Medipally

    2015-01-01

    Full Text Available The world energy crisis and increased greenhouse gas emissions have driven the search for alternative and environmentally friendly renewable energy sources. According to life cycle analysis, microalgae biofuel is identified as one of the major renewable energy sources for sustainable development, with potential to replace the fossil-based fuels. Microalgae biofuel was devoid of the major drawbacks associated with oil crops and lignocelluloses-based biofuels. Algae-based biofuels are technically and economically viable and cost competitive, require no additional lands, require minimal water use, and mitigate atmospheric CO2. However, commercial production of microalgae biodiesel is still not feasible due to the low biomass concentration and costly downstream processes. The viability of microalgae biodiesel production can be achieved by designing advanced photobioreactors, developing low cost technologies for biomass harvesting, drying, and oil extraction. Commercial production can also be accomplished by improving the genetic engineering strategies to control environmental stress conditions and by engineering metabolic pathways for high lipid production. In addition, new emerging technologies such as algal-bacterial interactions for enhancement of microalgae growth and lipid production are also explored. This review focuses mainly on the problems encountered in the commercial production of microalgae biofuels and the possible techniques to overcome these difficulties.

  9. Advice on the accelerated market implementation of advanced biofuels

    International Nuclear Information System (INIS)

    The Platform for Sustainable Mobility aims to promote the accelerated market introduction of more sustainable motor fuels and vehicle technology. The Platform distinguishes four transition paths: hybridization of the fleet of cars; implementation of biofuels; hydrogen-fuelled driving (driving on natural gas and biogas); intelligent transport systems (ITS). This advice involves part of the transition path for the implementation of biofuels, i.e. accelerated market introduction of advances biofuels. [mk

  10. Bio-fuel production potential in Romania

    International Nuclear Information System (INIS)

    The paper is based on the ESTO Study: Techno- Economic Feasibility of Large-Scale Production of Bio-Fuels in EU-Candidate Countries. Bio-fuel production has not been taken into account significantly until now in Romania, being limited to small- scale productions of ethanol, used mostly for various industrial purposes. However the climatic conditions and the quality of the soil are very suitable in the country for development of the main crops (wheat, sugar-beet, sunflower and rape-seed) used in bio-ethanol and bio-diesel production. The paper intended to consider a pertinent discussion of the present situation in Romania's agriculture stressing on the following essential items in the estimation of bio-fuels production potential: availability of feed-stock for bio-fuel production; actual productions of bio-fuels; fuel consumption; cost assessment; SWOT approach; expected trends. Our analysis was based on specific agricultural data for the period 1996-2000. An important ethanol potential (due to wheat, sugar-beet and maize cultures), as well as bio-diesel one (due to sun-flower and rape-seed) were predicted for the period 2005-2010 which could be exploited with the support of an important financial and technological effort, mainly from EU countries

  11. Comprehensive Evaluation of Algal Biofuel Production: Experimental and Target Results

    Directory of Open Access Journals (Sweden)

    Colin M. Beal

    2012-06-01

    Full Text Available Worldwide, algal biofuel research and development efforts have focused on increasing the competitiveness of algal biofuels by increasing the energy and financial return on investments, reducing water intensity and resource requirements, and increasing algal productivity. In this study, analyses are presented in each of these areas—costs, resource needs, and productivity—for two cases: (1 an Experimental Case, using mostly measured data for a lab-scale system, and (2 a theorized Highly Productive Case that represents an optimized commercial-scale production system, albeit one that relies on full-price water, nutrients, and carbon dioxide. For both cases, the analysis described herein concludes that the energy and financial return on investments are less than 1, the water intensity is greater than that for conventional fuels, and the amounts of required resources at a meaningful scale of production amount to significant fractions of current consumption (e.g., nitrogen. The analysis and presentation of results highlight critical areas for advancement and innovation that must occur for sustainable and profitable algal biofuel production can occur at a scale that yields significant petroleum displacement. To this end, targets for energy consumption, production cost, water consumption, and nutrient consumption are presented that would promote sustainable algal biofuel production. Furthermore, this work demonstrates a procedure and method by which subsequent advances in technology and biotechnology can be framed to track progress.

  12. Biorefineries for chemical and biofuel production

    DEFF Research Database (Denmark)

    Fjerbæk Søtoft, Lene

    with traditional land based food or feed crops, but can be grown to produce oil or biomass for biofuels as well as a long range of products with huge potential as food, feed or nutritionals. This with smaller requirements towards feed nutrients and land use. Value: If biofuels are to be used as a substitute...... for fossil fuel, the efficiency of the production process and product use must be increased substantially for the process to pay off as well economically as environmentally. Our knowledge base must thus be expanded and improved to include large production systems of i.e. whole-crop systems. Such research...

  13. Impacts of Climate Change on Biofuels Production

    Energy Technology Data Exchange (ETDEWEB)

    Melillo, Jerry M. [Marine Biological Laboratory, Woods Hole, MA (United States)

    2014-04-30

    The overall goal of this research project was to improve and use our biogeochemistry model, TEM, to simulate the effects of climate change and other environmental changes on the production of biofuel feedstocks. We used the improved version of TEM that is coupled with the economic model, EPPA, a part of MIT’s Earth System Model, to explore how alternative uses of land, including land for biofuels production, can help society meet proposed climate targets. During the course of this project, we have made refinements to TEM that include development of a more mechanistic plant module, with improved ecohydrology and consideration of plant-water relations, and a more detailed treatment of soil nitrogen dynamics, especially processes that add or remove nitrogen from ecosystems. We have documented our changes to TEM and used the model to explore the effects on production in land ecosystems, including changes in biofuels production.

  14. The potential of C4 grasses for cellulosic biofuel production

    Directory of Open Access Journals (Sweden)

    Tim eWeijde

    2013-05-01

    Full Text Available With the advent of biorefinery technologies enabling plant biomass to be processed into biofuel, many researchers set out to study and improve candidate biomass crops. Many of these candidates are C4 grasses, characterized by a high productivity and resource use efficiency. In this review the potential of five C4 grasses as lignocellulose feedstock for biofuel production is discussed. These include three important field crops - maize, sugarcane and sorghum - and two undomesticated perennial energy grasses - miscanthus and switchgrass. Although all these grasses are high yielding, they produce different products. While miscanthus and switchgrass are exploited exclusively for lignocellulosic biomass, maize, sorghum and sugarcane are dual-purpose crops. It is unlikely that all the prerequisites for the sustainable and economic production of biomass for a global cellulosic biofuel industry will be fulfilled by a single crop. High and stable yields of lignocellulose are required in diverse environments worldwide, to sustain a year-round production of biofuel. A high resource use efficiency is indispensable to allow cultivation with minimal inputs of nutrients and water and the exploitation of marginal soils for biomass production. Finally, the lignocellulose composition of the feedstock should be optimized to allow its efficient conversion into biofuel and other by-products. Breeding for these objectives should encompass diverse crops, to meet the demands of local biorefineries and provide adaptability to different environments. Collectively, these C4 grasses are likely to play a central role in the supply of lignocellulose for the cellulosic ethanol industry. Moreover, as these species are evolutionary closely related, advances in each of these crops will expedite improvements in the other crops. This review aims to provide an overview of their potential, prospects and research needs as lignocellulose feedstocks for the commercial production of

  15. A resilience perspective on biofuel production.

    Science.gov (United States)

    Mu, Dongyan; Seager, Thomas P; Rao, P Suresh C; Park, Jeryang; Zhao, Fu

    2011-07-01

    The recent investment boom and collapse of the corn ethanol industry calls into question the long-term sustainability of traditional approaches to biofuel technologies. Compared with petroleum-based transportation fuels, biofuel production systems are more closely connected to complex and variable natural systems. Especially as biofeedstock production itself becomes more independent of fossil fuel-based supports, stochasticity will become an increasingly important, inherent feature of biofuel feedstock production systems. Accordingly, a fundamental change in design philosophy is necessary to ensure the long-term viability of the biofuels industry. To respond effectively to unexpected disruptions, the new approach will require systems to be designed for resilience (indicated by diversity, efficiency, cohesion, and adaptability) rather than more narrowly defined measures of efficiency. This paper addresses important concepts in the design of coupled engineering-ecological systems (resistance, resilience, adaptability, and transformability) and examines biofuel conversion technologies from a resilience perspective. Conversion technologies that can accommodate multiple feedstocks and final products are suggested to enhance the diversity and flexibility of the entire industry. PMID:21309075

  16. Sustainable multipurpose biorefineries for third-generation biofuels and value-added co-products

    Science.gov (United States)

    Modern biorefinery facilities conduct many types of processes, including those producing advanced biofuels, commodity chemicals, biodiesel, and value-added co-products such as sweeteners and bioinsecticides, with many more co-products, chemicals and biofuels on the horizon. Most of these processes ...

  17. Role of Escherichia coli in Biofuel Production

    Science.gov (United States)

    Koppolu, Veerendra; Vasigala, Veneela KR

    2016-01-01

    Increased energy consumption coupled with depleting petroleum reserves and increased greenhouse gas emissions have renewed our interest in generating fuels from renewable energy sources via microbial fermentation. Central to this problem is the choice of microorganism that catalyzes the production of fuels at high volumetric productivity and yield from cheap and abundantly available renewable energy sources. Microorganisms that are metabolically engineered to redirect renewable carbon sources into desired fuel products are contemplated as best choices to obtain high volumetric productivity and yield. Considering the availability of vast knowledge in genomic and metabolic fronts, Escherichia coli is regarded as a primary choice for the production of biofuels. Here, we reviewed the microbial production of liquid biofuels that have the potential to be used either alone or in combination with the present-day fuels. We specifically highlighted the metabolic engineering and synthetic biology approaches used to improve the production of biofuels from E. coli over the past few years. We also discussed the challenges that still exist for the biofuel production from E. coli and their possible solutions. PMID:27441002

  18. Role of Escherichia coli in Biofuel Production.

    Science.gov (United States)

    Koppolu, Veerendra; Vasigala, Veneela Kr

    2016-01-01

    Increased energy consumption coupled with depleting petroleum reserves and increased greenhouse gas emissions have renewed our interest in generating fuels from renewable energy sources via microbial fermentation. Central to this problem is the choice of microorganism that catalyzes the production of fuels at high volumetric productivity and yield from cheap and abundantly available renewable energy sources. Microorganisms that are metabolically engineered to redirect renewable carbon sources into desired fuel products are contemplated as best choices to obtain high volumetric productivity and yield. Considering the availability of vast knowledge in genomic and metabolic fronts, Escherichia coli is regarded as a primary choice for the production of biofuels. Here, we reviewed the microbial production of liquid biofuels that have the potential to be used either alone or in combination with the present-day fuels. We specifically highlighted the metabolic engineering and synthetic biology approaches used to improve the production of biofuels from E. coli over the past few years. We also discussed the challenges that still exist for the biofuel production from E. coli and their possible solutions. PMID:27441002

  19. Renewable Energy Laboratory Development for Biofuels Advanced Combustion Studies

    Energy Technology Data Exchange (ETDEWEB)

    Soloiu, Valentin A. [Georgia Southern Univ., Statesboro, GA (United States)

    2012-03-31

    The research advanced fundamental science and applied engineering for increasing the efficiency of internal combustion engines and meeting emissions regulations with biofuels. The project developed a laboratory with new experiments and allowed investigation of new fuels and their combustion and emissions. This project supports a sustainable domestic biofuels and automotive industry creating economic opportunities across the nation, reducing the dependence on foreign oil, and enhancing U.S. energy security. The one year period of research developed fundamental knowledge and applied technology in advanced combustion, emissions and biofuels formulation to increase vehicle's efficiency. Biofuels combustion was investigated in a Compression Ignition Direct Injection (DI) to develop idling strategies with biofuels and an Indirect Diesel Injection (IDI) intended for auxiliary power unit.

  20. Omics in Chlamydomonas for Biofuel Production.

    Science.gov (United States)

    Aucoin, Hanna R; Gardner, Joseph; Boyle, Nanette R

    2016-01-01

    In response to demands for sustainable domestic fuel sources, research into biofuels has become increasingly important. Many challenges face biofuels in their effort to replace petroleum fuels, but rational strain engineering of algae and photosynthetic organisms offers a great deal of promise. For decades, mutations and stress responses in photosynthetic microbiota were seen to result in production of exciting high-energy fuel molecules, giving hope but minor capability for design. However, '-omics' techniques for visualizing entire cell processing has clarified biosynthesis and regulatory networks. Investigation into the promising production behaviors of the model organism C. reinhardtii and its mutants with these powerful techniques has improved predictability and understanding of the diverse, complex interactions within photosynthetic organisms. This new equipment has created an exciting new frontier for high-throughput, predictable engineering of photosynthetically produced carbon-neutral biofuels.

  1. Energy Crop and Biotechnology for Biofuel Production

    Institute of Scientific and Technical Information of China (English)

    Liangcai Peng; Neal Gutterson

    2011-01-01

    @@ Selection of energy crops is the first priority for large-scale biofuel production in China.As a major topic, it was extensively discussed in the Second International Symposium on Bioenergy and Biotechnology, held from October 16-19(th), 2010 in Huazhong Agricultural University(HZAU), Wuhan, China, with more than one hundred registered participants(Figure 1).

  2. A review on algal biofuel production

    Institute of Scientific and Technical Information of China (English)

    WANG Ling; ZHU jing

    2016-01-01

    Culturing of microalgae has be established as an alternative feedstock for biofuel production due to their fast growth rate and ability to accumulate high quantity of lipid and carbohydrate respectively. However, using this bioresource is still limited duo to low productivity and higher cultivation cost. Genetic and metabolic engineering,photobioreactors play significant role in algal biomass production. Hence, this review is focused on these, aiming at providing useful informations.

  3. Partnering with Industry to Advance Biofuels and Bioproducts (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    2011-12-01

    Fact sheet describing NREL's Integrated Biorefinery Research Facility, a biochemical pilot plant and partnership facility containing equipment and lab space for pretreatement, enzymatic hydrolysis, fermentation, compositional analysis, and downstream processing. For more than 30 years, the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) has been at the leading edge of research and technology advancements to develop renewable fuels and bioproducts. NREL works to develop cost-competitive alternatives to conventional transportation fuels and value-added biobased chemicals that can be used to manufacture clothing, plastics, lubricants, and other products. NREL is developing technologies and processes to produce a range of sustainable, energy-dense advanced biofuels that are compatible with our existing transportation fuel infrastructure. As part of that effort, NREL's National Bioenergy Center has entered into more than 90 collaborations in the past five years with companies ranging in size from start-ups to those that appear on Fortune magazine's Fortune 100 list. The new Integrated Biorefinery Research Facility (IBRF) showcases NREL's commitment to collaboration and to meeting the nation's biofuels and bioproducts development and deployment goals. Designed to speed the growth of the biofuels and bioproducts industries, the IBRF is a unique $33.5 million pilot facility capable of supporting a variety of projects. The IBRF is available to industry partners who work with NREL through cooperative research and development, technical, and analytical service agreements. With 27,000 ft2 of high bay space, the IBRF provides industry partners with the opportunity to operate, test, and develop their own biorefining technology and equipment.

  4. Biofuels and Their Co-Products as Livestock Feed: Global Economic and Environmental Implications.

    Science.gov (United States)

    Popp, József; Harangi-Rákos, Mónika; Gabnai, Zoltán; Balogh, Péter; Antal, Gabriella; Bai, Attila

    2016-01-01

    This review studies biofuel expansion in terms of competition between conventional and advanced biofuels based on bioenergy potential. Production of advanced biofuels is generally more expensive than current biofuels because products are not yet cost competitive. What is overlooked in the discussion about biofuel is the contribution the industry makes to the global animal feed supply and land use for cultivation of feedstocks. The global ethanol industry produces 44 million metric tonnes of high-quality feed, however, the co-products of biodiesel production have a moderate impact on the feed market contributing to just 8-9 million tonnes of protein meal output a year. By economically displacing traditional feed ingredients co-products from biofuel production are an important and valuable component of the biofuels sector and the global feed market. The return of co-products to the feed market has agricultural land use (and GHG emissions) implications as well. The use of co-products generated from grains and oilseeds can reduce net land use by 11% to 40%. The proportion of global cropland used for biofuels is currently some 2% (30-35 million hectares). By adding co-products substituted for grains and oilseeds the land required for cultivation of feedstocks declines to 1.5% of the global crop area. PMID:26938514

  5. Production of biofuels and chemicals with ionic liquids

    CERN Document Server

    Fang, Zhen; Qi, Xinhua

    2013-01-01

    This book explores the application of ionic liquids to biomass for producing biofuels and chemicals. Covers pretreatment, fermentation, cellulose transformation, reaction kinetics and more, as well as subsequent production of biofuels and platform chemicals.

  6. Positive and negative impacts of agricultural production of liquid biofuels

    NARCIS (Netherlands)

    L. Reijnders

    2012-01-01

    Agricultural production of liquid biofuels can have positive effects. It can decrease dependence on fossil fuels and increase farmers’ incomes. Agricultural production of mixed perennial biofuel crops may increase pollinator and avian richness. Most types of agricultural crop-based liquid biofuel pr

  7. Institutional analysis of biofuel production in Northern Ghana

    OpenAIRE

    Kwoyiga, Lydia

    2013-01-01

    The thesis studied the nature of institutional arrangement around biofuel production and how this arrangement has shaped the production outcome of biofuel companies and community development. The study was conducted in two communities of the Yendi Municipal Assembly of the Northern Region of Ghana. In this area, a biofuel company called Biofuel Africa Limited has acquired areas of land and cultivated Jatropha plantations. A total of 32 informants were interviewed to arrive at information ne...

  8. Fermentation broth components influence droplet coalescence and hinder advanced biofuel recovery during fermentation

    NARCIS (Netherlands)

    Heeres, A.S.; Schroën, K.; Heijnen, J.J.; Van der Wielen, L.A.M.; Cuellar, M.C.

    2015-01-01

    Developments in synthetic biology enabled the microbial production of long chain hydrocarbons, which can be used as advanced biofuels in aviation or transportation. Currently, these fuels are not economically competitive due to their production costs. The current process offers room for improvement:

  9. Production of biofuels obtained from microalgae

    OpenAIRE

    Luis Carlos Fernández-Linares; Jorge Montiel Montoya; Aarón Millán Oropeza; Jesús Agustín Badillo Corona

    2012-01-01

    A review of the situation of bio-fuels in the world, mainly of biodiesel is made. A comparison among the different raw materials for the synthesis of biodiesel is done and it is emphasized in the production of biodiesel from microalgae. The different fresh and salt water micro-algae in its lipid content and productivity are compared. A review of the process of biosynthesis of lipids in microalgae and how to improve the production of lipids in microalgae is shown. It is discussed the importanc...

  10. Prospective and development of butanol as an advanced biofuel.

    Science.gov (United States)

    Xue, Chuang; Zhao, Xin-Qing; Liu, Chen-Guang; Chen, Li-Jie; Bai, Feng-Wu

    2013-12-01

    Butanol has been acknowledged as an advanced biofuel, but its production through acetone-butanol-ethanol (ABE) fermentation by clostridia is still not economically competitive, due to low butanol yield and titer. In this article, update progress in butanol production is reviewed. Low price and sustainable feedstocks such as lignocellulosic residues and dedicated energy crops are needed for butanol production at large scale to save feedstock cost, but processes are more complicated, compared to those established for ABE fermentation from sugar- and starch-based feedstocks. While rational designs targeting individual genes, enzymes or pathways are effective for improving butanol yield, global and systems strategies are more reasonable for engineering strains with stress tolerance controlled by multigenes. Compared to solvent-producing clostridia, engineering heterologous species such as Escherichia coli and Saccharomyces cerevisiae with butanol pathway might be a solution for eliminating the formation of major byproducts acetone and ethanol so that butanol yield can be improved significantly. Although batch fermentation has been practiced for butanol production in industry, continuous operation is more productive for large scale production of butanol as a biofuel, but a single chemostat bioreactor cannot achieve this goal for the biphasic ABE fermentation, and tanks-in-series systems should be optimized for alternative feedstocks and new strains. Moreover, energy saving is limited for the distillation system, even total solvents in the fermentation broth are increased significantly, since solvents are distilled to ~40% by the beer stripper, and more than 95% water is removed with the stillage without phase change, even with conventional distillation systems, needless to say that advanced chemical engineering technologies can distil solvents up to ~90% with the beer stripper, and the multistage pressure columns can well balance energy consumption for solvent fraction

  11. Prospective and development of butanol as an advanced biofuel.

    Science.gov (United States)

    Xue, Chuang; Zhao, Xin-Qing; Liu, Chen-Guang; Chen, Li-Jie; Bai, Feng-Wu

    2013-12-01

    Butanol has been acknowledged as an advanced biofuel, but its production through acetone-butanol-ethanol (ABE) fermentation by clostridia is still not economically competitive, due to low butanol yield and titer. In this article, update progress in butanol production is reviewed. Low price and sustainable feedstocks such as lignocellulosic residues and dedicated energy crops are needed for butanol production at large scale to save feedstock cost, but processes are more complicated, compared to those established for ABE fermentation from sugar- and starch-based feedstocks. While rational designs targeting individual genes, enzymes or pathways are effective for improving butanol yield, global and systems strategies are more reasonable for engineering strains with stress tolerance controlled by multigenes. Compared to solvent-producing clostridia, engineering heterologous species such as Escherichia coli and Saccharomyces cerevisiae with butanol pathway might be a solution for eliminating the formation of major byproducts acetone and ethanol so that butanol yield can be improved significantly. Although batch fermentation has been practiced for butanol production in industry, continuous operation is more productive for large scale production of butanol as a biofuel, but a single chemostat bioreactor cannot achieve this goal for the biphasic ABE fermentation, and tanks-in-series systems should be optimized for alternative feedstocks and new strains. Moreover, energy saving is limited for the distillation system, even total solvents in the fermentation broth are increased significantly, since solvents are distilled to ~40% by the beer stripper, and more than 95% water is removed with the stillage without phase change, even with conventional distillation systems, needless to say that advanced chemical engineering technologies can distil solvents up to ~90% with the beer stripper, and the multistage pressure columns can well balance energy consumption for solvent fraction

  12. Production of biofuels obtained from microalgae

    Directory of Open Access Journals (Sweden)

    Luis Carlos Fernández-Linares

    2012-09-01

    Full Text Available A review of the situation of bio-fuels in the world, mainly of biodiesel is made. A comparison among the different raw materials for the synthesis of biodiesel is done and it is emphasized in the production of biodiesel from microalgae. The different fresh and salt water micro-algae in its lipid content and productivity are compared. A review of the process of biosynthesis of lipids in microalgae and how to improve the production of lipids in microalgae is shown. It is discussed the importance of the genetic manipulation to highly lipid-producing microalgae (example: Botryrococuus braunni, Nannochloropsis sp, Noechlorisoleobundans and Nitschia sp.. A study of the advantages and disadvantages of the different systems of cultivation of microalgae is also made. Finally, it is shown a perspective of biofuels from microalgae. Among the main challenges to overcome to produce biodiesel from microalgae are: the cost of production of biomass, which involves the optimization of media, selection and manipulation of strains and photobioreactors design. The processof separation of biomass, the extraction of oils and by-products, the optimization of the process of transesterification, purification and use of by-products must also be considered.

  13. Fatty acid-derived biofuels and chemicals production in Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Yongjin J. Zhou

    2014-09-01

    Full Text Available Volatile energy costs and environmental concerns have spurred interest in the development of alternative, renewable, sustainable and cost-effective energy resources. Advanced biofuels have potential to replace fossil fuels in supporting high-power demanding machinery such as aircrafts and trucks. Microbial biosynthesis is generally considered as an environmental friendly refinery process, and fatty acid biosynthesis is an attractive route to synthesize chemicals and especially drop-in biofuels due to the high degree of reduction of fatty acids. The robustness and excellent accessibility to molecular genetics make the yeast S. cerevisiae a suitable host for the production of biofuels, chemicals and pharmaceuticals, and recent advances in metabolic engineering as well as systems and synthetic biology allow us to engineer the yeast fatty acid metabolism and modification pathways for production of advanced biofuels and chemicals.

  14. Advanced biofuels in a biorefinery approach

    Energy Technology Data Exchange (ETDEWEB)

    Joergensen, Henning (ed.)

    2012-07-01

    The conference was organized by the University of Copenhagen and Bio4Bio, a centre for development and implementation of biotechnology for bioenergy consisting of 8 Danish universities and companies. The conference was also coordinated with the International Energy Agency's two networks: Bioenergy Task 39 (commercializing liquid biofuels from biomass) and Task 42 (biorefineries). The conference was organized in the following sessions: Biorefineries seen from an ecological, economical and industrial point of view; Plants and biomass - input for the biorefinery; Biomass processing and pretreatment; Enzymatic hydrolysis in biorefineries; Sustainability; Fermentation technologies and systems biology; Biomass recalcitrance and conversion; Biorefinery technologies and integration; IEA Bioenergy Task 42; IEA Bioenergy Task 39; New materials and chemicals from biomass; Deployment of biorefinery technologies. The present report contains oral and poster abstracts, and most of them are indexed separately. (Author)

  15. Status of advanced biofuels demonstration facilities in 2012. A report to IEA Bioenergy task 39

    Energy Technology Data Exchange (ETDEWEB)

    Bacovsky, Dina; Ludwiczek, Nikolaus; Ognissanto, Monica; Woergetter, Manfred

    2013-03-18

    A number of companies around the world pursue projects to develop and deploy advanced technologies for the production of biofuels. Plenty of options are available, e.g. on which feedstock to use, how to pretreat it and how to convert it, up to which fuel to produce. This report monitors the multi-facetted development, adds transparency to the sector and thus supports the development and deployment of advanced biofuels production technologies. Main pathways under development can be classified into biochemical technologies, thermochemical technologies and chemical technologies. Biochemical technologies are usually based on lignocellulosic feedstock which is pretreated, hydrolysed into sugars and then fermented to ethanol. Alternative biochemical pathways process sugars or gaseous components into methanol, butanol, mixed alcohols, acetic acids, or other chemical building blocks. Most thermochemical technologies use gasification to convert lignocellulosic feedstock into synthesis gas, which can be converted into BtL-Diesel, SNG, DME or mixed alcohols. Alternative thermochemical pathways include pyrolysis of biomass and upgrading of the resulting pyrolysis oil. The most successful chemical pathway is the hydrotreatment of vegetable oil or fats to produce diesel-type hydrocarbons. Other pathways include catalytic decarboxylation, and methanol production from glycerin. This report is based on a database on advanced biofuels projects. The database feeds into an interactive map which is available at http://demoplants.bioenergy2020.eu, and it is updated continuously. The report includes general descriptions of the main advanced biofuels technologies under development, a list of 102 projects that are being pursued worldwide, and detailed descriptions of these projects. All data displayed has been made available by the companies that pursue these projects. For this reason, the list of projects may not be complete, as some companies may still be reluctant to share data. Since

  16. Metabolic engineering of microalgal based biofuel production: prospects and challenges

    OpenAIRE

    Chiranjib eBanerjee; Kashyap Kumar Dubey; Pratyoosh eShukla

    2016-01-01

    The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than other sources based on plant seeds. However, the greatest challenge in a transition to algae-based biofuel production is the various other complications involved in microalgal cultivation, its harve...

  17. Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges

    OpenAIRE

    Banerjee, Chiranjib; Dubey, Kashyap K.; Shukla, Pratyoosh

    2016-01-01

    The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than other sources based on plant seeds. However, the greatest challenge in a transition to algae-based biofuel production is the various other complications involved in microalgal cultivation, its harve...

  18. Hydrodynamics-Biology Coupling for Algae Culture and Biofuel Production

    OpenAIRE

    Bernard, Olivier; Sainte-Marie, Jacques; Sialve, Bruno; Steyer, Jean-Philippe

    2013-01-01

    Biofuel production from microalgae represents an acute optimization problem for industry. There is a wide range of parameters that must be taken into account in the development of this technology. Here, mathematical modelling has a vital role to play. The potential of microalgae as a source of biofuel and as a technological solution for CO2 fixation is the subject of intense academic and industrial research. Large-scale production of microalgae has potential for biofuel applications owing to ...

  19. Limitation of Biofuel Production in Europe from the Forest Market

    Science.gov (United States)

    Leduc, Sylvain; Wetterlund, Elisabeth; Dotzauer, Erik; Kindermann, Georg

    2013-04-01

    The European Union has set a 10% target for the share of biofuel in the transportation sector to be met by 2020. To reach this target, second generation biofuel is expected to replace 3 to 5% of the transport fossil fuel consumption. But the competition on the feedstock is an issue and makes the planning for the second generation biofuel plant a challenge. Moreover, no commercial second generation biofuel production plant is under operation, but if reaching commercial status, this type of production plants are expected to become very large. In order to minimize the tranportation costs and to takle the competetion for the feedstock against the existing woody based industries, the geographical location of biofuel production plants becomes an issue. This study investigates the potential of second generation biofuel economically feasible in Europe by 2020 in regards with the competition for the feedsstock with the existing woody biomass based industries (CHP, pulp and paper mills, sawmills...). To assess the biofuel potential in Europe, a techno-economic, geographically explicit model, BeWhere, is used. It determines the optimal locations of bio-energy production plants by minimizing the costs and CO2 emissions of the entire supply chain. The existing woody based industries have to first meet their wood demand, and if the amount of wood that remains is suficiant, new bio-energy production plants if any can be set up. Preliminary results show that CHP plants are preferably chosen over biofuel production plants. Strong biofuel policy support is needed in order to consequently increase the biofuel production in Europe. The carbon tax influences the emission reduction to a higher degree than the biofuel support. And the potential of second generation biofuel would at most reach 3% of the European transport fuel if the wood demand does not increase from 2010.

  20. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

    Energy Technology Data Exchange (ETDEWEB)

    Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

    2009-12-02

    The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

  1. Improving butanol fermentation to enter the advanced biofuel market.

    Science.gov (United States)

    Tracy, Bryan P

    2012-01-01

    1-Butanol is a large-volume, intermediate chemical with favorable physical and chemical properties for blending with or directly substituting for gasoline. The per-volume value of butanol, as a chemical, is sufficient for investing into the recommercialization of the classical acetone-butanol-ethanol (ABE) (E. M. Green, Curr. Opin. Biotechnol. 22:337-343, 2011) fermentation process. Furthermore, with modest improvements in three areas of the ABE process, operating costs can be sufficiently decreased to make butanol an economically viable advanced biofuel. The three areas of greatest interest are (i) maximizing yields of butanol on any particular substrate, (ii) expanding substrate utilization capabilities of the host microorganism, and (iii) reducing the energy consumption of the overall production process, in particular the separation and purification operations. In their study in the September/October 2012 issue of mBio, Jang et al. [mBio 3(5):e00314-12, 2012] describe a comprehensive study on driving glucose metabolism in Clostridium acetobutylicum to the production of butanol. Moreover, they execute a metabolic engineering strategy to achieve the highest yet reported yields of butanol on glucose. PMID:23232720

  2. Protein engineering in designing tailored enzymes and microorganisms for biofuels production.

    Science.gov (United States)

    Wen, Fei; Nair, Nikhil U; Zhao, Huimin

    2009-08-01

    Lignocellulosic biofuels represent a sustainable, renewable, and the only foreseeable alternative energy source to transportation fossil fuels. However, the recalcitrant nature of lignocellulose poses technical hurdles to an economically viable biorefinery. Low enzymatic hydrolysis efficiency and low productivity, yield, and titer of biofuels are among the top cost contributors. Protein engineering has been used to improve the performance of lignocellulose-degrading enzymes, as well as proteins involved in biofuel synthesis pathways. Unlike its great success seen in other industrial applications, protein engineering has achieved only modest results in improving the lignocellulose-to-biofuels efficiency. This review will discuss the unique challenges that protein engineering faces in the process of converting lignocellulose to biofuels and how they are addressed by recent advances in this field.

  3. Livelihood implications of biofuel crop production: Implications for governance

    DEFF Research Database (Denmark)

    Hunsberger, Carol; Bolwig, Simon; Corbera, Esteve;

    2014-01-01

    While much attention has focused on the climate change mitigation potential of biofuels, research from the social sciences increasingly highlights the social and livelihood impacts of their expanded production. Policy and governance measures aimed at improving the social effects of biofuels have...... by their cultivation in the global South – income, food security, access to land-based resources, and social assets – revealing that distributional effects are crucial to evaluating the outcomes of biofuel production across these dimensions. Second, we ask how well selected biofuel governance mechanisms address...

  4. Rapid saccharification for production of cellulosic biofuels.

    Science.gov (United States)

    Lee, Dae-Seok; Wi, Seung Gon; Lee, Soo Jung; Lee, Yoon-Gyo; Kim, Yeong-Suk; Bae, Hyeun-Jong

    2014-04-01

    The economical production of biofuels is hindered by the recalcitrance of lignocellulose to processing, causing high consumption of processing enzymes and impeding hydrolysis of pretreated lignocellulosic biomass. We determined the major rate-limiting factor in the hydrolysis of popping pre-treated rice straw (PPRS) by examining cellulase adsorption to lignin and cellulose, amorphogenesis of PPRS, and re-hydrolysis. Based on the results, equivalence between enzyme loading and the open structural area of cellulose was required to significantly increase productive adsorption of cellulase and to accelerate enzymatic saccharification of PPRS. Amorphogenesis of PPRS by phosphoric acid treatment to expand open structural area of the cellulose fibers resulted in twofold higher cellulase adsorption and increased the yield of the first re-hydrolysis step from 13% to 46%. The total yield from PPRS was increased to 84% after 3h. These results provide evidence that cellulose structure is one of major effects on the enzymatic hydrolysis. PMID:24607460

  5. A modelling approach to estimate the European biofuel production: from crops to biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Clodic, Melissa [Institute National de la Recherche Agronomique (IFP/INRA), Paris (France). Instituto Frances do Petroleo

    2008-07-01

    Today, in the context of energy competition and climate change, biofuels are promoted as a renewable resource to diversify the energy supply. However, biofuel development remains controversial. Here, we will present a way to make an environmental and economic cost and benefit analysis of European biofuels, from the crops until the marketed products, by using a linear programming optimization modelling approach. To make this European biofuel production model, named AGRAF, possible, we decided to use different independent linear programming optimization models which represent the separate parts of the process: European agricultural production, production of transforming industries and refinery production. To model the agricultural and the refining sections, we have chosen to improve existing and experimented models by adding a biofuel production part. For the transforming industry, we will create a new partial equilibrium model which will represent stake holders such as Sofiproteol, Stereos, etc. Data will then be exchanged between the models to coordinate all the biofuel production steps. Here, we will also focus on spatialization in order to meet certain of our requirements, such as the exchange flux analysis or the determination of transport costs, usually important in an industrial optimization model. (author)

  6. Optimal Localization of Biofuel Production on a European Scale

    OpenAIRE

    Wetterlund, E.

    2010-01-01

    Second generation biofuels use non-food lignocellulosic feedstock, for example waste or forest residues, and have in general lower environmental impact than first generation biofuels. In order to reach the 2020 target of 10% renewable energy in transport it will likely be necessary to have a share of at least 3% second generation fuels in the EU fuel mix. However, second generation biofuel production plants will typically need to be very large which puts significant demand on the supply chain...

  7. The economic and environmental sustainability of biofuel production

    OpenAIRE

    Annalisa Zezza

    2007-01-01

    This paper analyses some of the determinants of biofuel economy in terms of relations with the fossil fuels markets and the agricultural markets. In particular, on the basis of the scientific literature the impact of fossil fuel prices on the agricultural markets is analyzed together with substitutability between food/fodder and biofuel production. The study then goes on to attempt evaluation of the environmental sustainability of biofuels on the basis of the energy balance, greenhouse gas re...

  8. An integrated approach: advances in the use of Clostridium for biofuel.

    Science.gov (United States)

    Kök, M Samil

    2015-01-01

    Almost 90% of our energy comes from fossil fuels, which are both limited and polluting, hence the need to find alternative sources. Biofuels can provide a sustainable and renewable source of energy for the future. Recent significant advances in genetic engineering and fermentation technology have made microbial bio-based production of chemicals from renewable resources more viable. Clostridium species are considered as promising micro-organisms for the production of a wide range of chemicals for industrial use. However, a number of scientific challenges still need to be overcome to facilitate an economically viable production system. These include the use of cheap non-food-based substrates, a better understanding of the metabolic processes involved, improvement of strains through genetic engineering and innovation in process technology. This paper reviews recent developments in these areas, advancing the use of Clostridium within an industrial context especially for the production of biofuels. PMID:27160660

  9. Recent developments on biofuels production from microalgae and macroalgae

    DEFF Research Database (Denmark)

    Kumar, Kanhaiya; Ghosh, Supratim; Angelidaki, Irini;

    2016-01-01

    Biofuels from algae are considered as promising alternatives of conventional fossil fuels, as they can eliminate most of the environmental problems. The present study focuses on all the possible avenues of biofuels production through biochemical and thermochemical conversion methods in one place,...

  10. Global nitrogen requirement for increased biofuel production

    NARCIS (Netherlands)

    Flapper, Joris

    2008-01-01

    Biofuels are thought to be one of the options to substitute fossil fuels and prevent global warming by the greenhouse gas (GHG) effect as they are seen as a renewable form of energy. However, biofuels are almost solely subjected to criticism from an energ

  11. Next generation of liquid biofuel production

    NARCIS (Netherlands)

    Batidzirai, B.

    2012-01-01

    More than 99% of all currently produced biofuels are classified as “first generation” (i.e. fuels produced primarily from cereals, grains, sugar crops and oil seeds) (IEA, 2008b). “Second generation” or “next generation” biofuels, on the other hand, are produced from lignocellulosic feedstocks such

  12. Recent trends in nanomaterials immobilised enzymes for biofuel production.

    Science.gov (United States)

    Verma, Madan L; Puri, Munish; Barrow, Colin J

    2016-01-01

    Application of nanomaterials as novel supporting materials for enzyme immobilisation has generated incredible interest in the biotechnology community. These robust nanostructured forms, such as nanoparticles, nanofibres, nanotubes, nanoporous, nanosheets, and nanocomposites, possess a high surface area to volume ratios that can cause a high enzyme loading and facilitate reaction kinetics, thus improving biocatalytic efficiency for industrial applications. In this article, we discuss research opportunities of nanoscale materials in enzyme biotechnology and highlight recent developments in biofuel production using advanced material supports for enzyme immobilisation and stabilisation. Synthesis and functionalisation of nanomaterial forms using different methods are highlighted. Various simple and effective strategies designed to result in a stable, as well as functional protein-nanomaterial conjugates are also discussed. Analytical techniques confirming enzyme loading on nanomaterials and assessing post-immobilisation changes are discussed. The current status of versatile nanomaterial support for biofuel production employing cellulases and lipases is described in details. This report concludes with a discussion on the likely outcome that nanomaterials will become an integral part of sustainable bioenergy production.

  13. The National Biofuels Strategy - Importance of sustainable feedstock production systems in regional-based supply chains

    Science.gov (United States)

    Region-based production systems are needed to produce the feedstocks that will be turned into the biofuels required to meet Federal mandated targets. Executive and Legislative actions have put into motion significant government responses designed to advance the development and production of domestic...

  14. Optimal localisation of next generation biofuel production in Sweden

    OpenAIRE

    Wetterlund, Elisabeth; Pettersson, Karin; Mossberg, Johanna; Torén, Johan; Hoffstedt, Christian; von Schenck, Anna; Berglin, Niklas; Lundmark, Robert; Lundgren, Joakim; Leduc, Sylvain; Kindermann, Georg

    2013-01-01

    With a high availability of lignocellulosic biomass and various types of cellulosic by-products, as well as a large number of industries, Sweden is a country of great interest for future large scale production of sustainable, next generation biofuels. This is most likely also a necessity as Sweden has the ambition to be independent of fossil fuels in the transport sector by the year 2030 and completely fossil free by 2050. In order to reach competitive biofuel production costs, plants with la...

  15. Next generation of liquid biofuel production

    OpenAIRE

    Batidzirai, B.

    2012-01-01

    More than 99% of all currently produced biofuels are classified as “first generation” (i.e. fuels produced primarily from cereals, grains, sugar crops and oil seeds) (IEA, 2008b). “Second generation” or “next generation” biofuels, on the other hand, are produced from lignocellulosic feedstocks such as agricultural and forest residues, as well as purpose-grown energy crops such as vegetative grasses and short rotation forests (SRF). These feedstocks largely consist of cellulose, hemicellulose ...

  16. Biofuel Crops Expansion: Evaluating the Impact on the Agricultural Water Scarcity Costs and Hydropower Production with Hydro Economic Modeling

    Science.gov (United States)

    Marques, G.

    2015-12-01

    Biofuels such as ethanol from sugar cane remain an important element to help mitigate the impacts of fossil fuels on the atmosphere. However, meeting fuel demands with biofuels requires technological advancement for water productivity and scale of production. This may translate into increased water demands for biofuel crops and potential for conflicts with incumbent crops and other water uses including domestic, hydropower generation and environmental. It is therefore important to evaluate the effects of increased biofuel production on the verge of water scarcity costs and hydropower production. The present research applies a hydro-economic optimization model to compare different scenarios of irrigated biofuel and hydropower production, and estimates the potential tradeoffs. A case study from the Araguari watershed in Brazil is provided. These results should be useful to (i) identify improved water allocation among competing economic demands, (ii) support water management and operations decisions in watersheds where biofuels are expected to increase, and (iii) identify the impact of bio fuel production in the water availability and economic value. Under optimized conditions, adoption of sugar cane for biofuel production heavily relies on the opportunity costs of other crops and hydropower generation. Areas with a lower value crop groups seem more suitable to adopt sugar cane for biofuel when the price of ethanol is sufficiently high and the opportunity costs of hydropower productions are not conflicting. The approach also highlights the potential for insights in water management from studying regional versus larger scales bundled systems involving water use, food production and power generation.

  17. The Production of Biofuels: Welfare and Environmental Consequences for Asia

    OpenAIRE

    Clement A. Tisdell

    2009-01-01

    The production of biofuels has been supported by many conservationists and environmentalists on the grounds that it reduces greenhouse gas emissions and is a renewable energy substitute for non-renewable fossil fuels, mainly oil. More recently the domestic production of biofuels (and the domestic supply of other forms of alternative energy) have been welcomed by several nations as ways to reduce their oil imports and increase their energy self-sufficiency, as for example, has happened in the ...

  18. DMF - A New Biofuel Candidate

    OpenAIRE

    Tian, Guohong; Daniel, Ritchie; Xu, Hongming

    2011-01-01

    This book aspires to be a comprehensive summary of current biofuels issues and thereby contribute to the understanding of this important topic. Readers will find themes including biofuels development efforts, their implications for the food industry, current and future biofuels crops, the successful Brazilian ethanol program, insights of the first, second, third and fourth biofuel generations, advanced biofuel production techniques, related waste treatment, emissions and environmental impacts...

  19. World Biofuels Production Potential Understanding the Challenges to Meeting the U.S. Renewable Fuel Standard

    Energy Technology Data Exchange (ETDEWEB)

    Sastri, B.; Lee, A.

    2008-09-15

    . Within the mandate, amounts of advanced biofuels, including biomass-based diesel and cellulosic biofuels, are required beginning in 2009. Imported renewable fuels are also eligible for the RFS. Another key U.S. policy is the $1.01 per gal tax credit for producers of cellulosic biofuels enacted as part of the 2008 Farm Bill. This credit, along with the DOE's research, development and demonstration (RD&D) programs, are assumed to enable the rapid expansion of U.S. and global cellulosic biofuels production needed for the U.S. to approach the 2022 RFS goal. While the Environmental Protection Agency (EPA) has yet to issue RFS rules to determine which fuels would meet the greenhouse gas (GHG) reduction and land use restrictions specified in EISA, we assume that cellulosic ethanol, biomass-to-liquid fuels (BTL), sugar-derived ethanol, and fatty acid methyl ester biodiesel would all meet the EISA advanced biofuel requirements. We also assume that enough U.S. corn ethanol would meet EISA's biofuel requirements or otherwise be grandfathered under EISA to reach 15 B gal per year.

  20. Feasibilities of consolidated bioprocessing microbes: from pretreatment to biofuel production.

    Science.gov (United States)

    Parisutham, Vinuselvi; Kim, Tae Hyun; Lee, Sung Kuk

    2014-06-01

    Lignocelluloses are rich sugar treasures, which can be converted to useful commodities such as biofuel with the help of efficient combination of enzymes and microbes. Although several bioprocessing approaches have been proposed, biofuel production from lignocelluloses is limited because of economically infeasible technologies for pretreatment, saccharification and fermentation. Use of consolidated bioprocessing (CBP) microbes is the most promising method for the cost-effective production of biofuels. However, lignocelluloses are obtained from highly diverse environment and hence are heterogeneous in nature. Therefore, it is necessary to develop and integrate tailor-designed pretreatment processes and efficient microbes that can thrive on many different kinds of biomass. In this review, the progress towards the construction of consolidated bioprocessing microbes, which can efficiently convert heterogeneous lignocellulosic biomass to bioenergy, has been discussed; in addition, the potential and constraints of current bioprocessing technologies for cellulosic biofuel production have been discussed.

  1. Microbiological aspects of biofuel production: Current status and future directions

    Directory of Open Access Journals (Sweden)

    Mostafa S. Elshahed

    2010-04-01

    Full Text Available Biofuel research is currently an area of immense interest due to the increase in global energy demand by emerging economies and the recent increases in global oil prices. Multiple approaches are currently being researched for the use of microorganisms in the production of various biofuel (e.g. alcohols, hydrogen, biodiesel, and biogas from multiple starting materials. This review provides a brief overview on the research currently underway on laboratory and industrial scales in the area of biofuels, with specific emphasis on the economic viability of various approaches currently being utilized.

  2. Biofuel technologies. Recent developments

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, Vijai Kumar [National Univ. of Ireland Galway (Ireland). Dept. of Biochemistry; MITS Univ., Rajasthan (India). Dept. of Science; Tuohy, Maria G. (eds.) [National Univ. of Ireland Galway (Ireland). Dept. of Biochemistry

    2013-02-01

    Written by experts. Richly illustrated. Of interest to both experienced researchers and beginners in the field. Biofuels are considered to be the main potential replacement for fossil fuels in the near future. In this book international experts present recent advances in biofuel research and related technologies. Topics include biomethane and biobutanol production, microbial fuel cells, feedstock production, biomass pre-treatment, enzyme hydrolysis, genetic manipulation of microbial cells and their application in the biofuels industry, bioreactor systems, and economical processing technologies for biofuel residues. The chapters provide concise information to help understand the technology-related implications of biofuels development. Moreover, recent updates on biofuel feedstocks, biofuel types, associated co- and byproducts and their applications are highlighted. The book addresses the needs of postgraduate researchers and scientists across diverse disciplines and industrial sectors in which biofuel technologies and related research and experimentation are pursued.

  3. 75 FR 21191 - Subpart B-Advanced Biofuel Payment Program; Correction

    Science.gov (United States)

    2010-04-23

    ..., 2010 at 75 FR 20085 proposing a payment program for producers of advanced biofuels to supporting.... SUPPLEMENTARY INFORMATION: Correction In the Federal Register of April 16, 2010, in FR Doc. 2010-8278, on page...--Advanced Biofuel Payment Program; Correction AGENCY: Rural Business-Cooperative Service, USDA....

  4. Biofuel production from microalgae as feedstock: current status and potential.

    Science.gov (United States)

    Han, Song-Fang; Jin, Wen-Biao; Tu, Ren-Jie; Wu, Wei-Min

    2015-06-01

    Algal biofuel has become an attractive alternative of petroleum-based fuels in the past decade. Microalgae have been proposed as a feedstock to produce biodiesel, since they are capable of mitigating CO2 emission and accumulating lipids with high productivity. This article is an overview of the updated status of biofuels, especially biodiesel production from microalgae including fundamental research, culture selection and engineering process development; it summarizes research on mathematical and life cycle modeling on algae growth and biomass production; and it updates global efforts of research and development and commercialization attempts. The major challenges are also discussed.

  5. PRODUCTION OF BIOFUELS WITH CORN: A WELFARE ANALYSIS IN MEXICO

    OpenAIRE

    Salvador González-Andrade; Alejandro Brugués-Rodríguez

    2010-01-01

    According to the decrease in the global offer and the increase of prices of fossil fuels exists a growing trend to generate energy alternatives. The production of commodities to generate biofuels competes with the production of foods, fibers and wood. The ethanol production from grains as corn and weath will impact the global market of food. In the United States, the main supplier of yellow corn to Mexico, in 2012 is foreseen to use a mixture of biofuels with ten percent of ethanol (OECD, 200...

  6. Genes related to xylose fermentation and methods of using same for enhanced biofuel production

    Science.gov (United States)

    Wohlbach, Dana J.; Gasch, Audrey P.

    2014-08-05

    The present invention provides isolated gene sequences involved in xylose fermentation and related recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast useful for biofuel production are also provided.

  7. Genes related to xylose fermentation and methods of using same for enhanced biofuel production

    Energy Technology Data Exchange (ETDEWEB)

    Wohlbach, Dana J.; Gasch, Audrey P.

    2015-09-29

    The present invention provides isolated gene sequences involved in xylose fermentation and related recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast useful for biofuel production are also provided.

  8. Membrane technology in production of biofuels : tried-and-tested technology improves new biofuel processes

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2010-07-15

    Membrane filtration technology, long used in many industrial process streams, is now being adopted in biofuels production and integrated biorefineries, particularly in second generation cellulosic ethanol processes. Second-generation bio-ethanol processes seek to optimize fuel recovery and secondary products from the feedstock and obtain a better value fuel. Membranes are being used to improve bioprocesses, lower energy costs, and increase product recovery. Membranes are engineered physical barriers used in processes for liquid/liquid and liquid/solid separation, permitting the passage of materials only up to a certain size, shape, or character. In biodiesel processes, membranes are being increasingly used to facilitate water reuse. The technology is being explored for use in the production of organic acids, which can form the base for biodegradable plastics. Integrated biorefineries are using microfiltration, ultrafiltration, nanofiltration, and reverse osmosis. Membranes are being used in fermentation with mesophilic and thermophilic organisms to produce biofuels and organic and amino acids. Membrane technology is low cost relative to using evaporators for recovering or removing water, and it is promising for continuous fermentation, as it helps retain microbial biomass in the fermenter while allowing liquid to be drawn out continuously. Membrane technology developed for use at wastewater treatment plants is being applied in biodiesel production, which produces wash water that is high in contaminants. Membrane technology is part of a wave of biofuel research and demonstration plants.

  9. Bioeconomic Sustainability of Cellulosic Biofuel Production on Marginal Lands

    Science.gov (United States)

    Gutierrez, Andrew Paul; Ponti, Luigi

    2009-01-01

    The use of marginal land (ML) for lignocellulosic biofuel production is examined for system stability, resilience, and eco-social sustainability. A North American prairie grass system and its industrialization for maximum biomass production using biotechnology and agro-technical inputs is the focus of the analysis. Demographic models of ML biomass…

  10. Managing water resources for biomass production in a biofuel economy

    Science.gov (United States)

    One goal of our national security policy is to become more energy independent using biofuels. The expanded production of agricultural crops for bioenergy production has introduced new challenges for management of water. Water availability has been widely presumed in the discussion of bioenergy crop ...

  11. Sustainability of biofuels and renewable chemicals production from biomass.

    Science.gov (United States)

    Kircher, Manfred

    2015-12-01

    In the sectors of biofuel and renewable chemicals the big feedstock demand asks, first, to expand the spectrum of carbon sources beyond primary biomass, second, to establish circular processing chains and, third, to prioritize product sectors exclusively depending on carbon: chemicals and heavy-duty fuels. Large-volume production lines will reduce greenhouse gas (GHG) emission significantly but also low-volume chemicals are indispensable in building 'low-carbon' industries. The foreseeable feedstock change initiates innovation, securing societal wealth in the industrialized world and creating employment in regions producing biomass. When raising the investments in rerouting to sustainable biofuel and chemicals today competitiveness with fossil-based fuel and chemicals is a strong issue. Many countries adopted comprehensive bioeconomy strategies to tackle this challenge. These public actions are mostly biased to biofuel but should give well-balanced attention to renewable chemicals as well.

  12. Sustainability of biofuels and renewable chemicals production from biomass.

    Science.gov (United States)

    Kircher, Manfred

    2015-12-01

    In the sectors of biofuel and renewable chemicals the big feedstock demand asks, first, to expand the spectrum of carbon sources beyond primary biomass, second, to establish circular processing chains and, third, to prioritize product sectors exclusively depending on carbon: chemicals and heavy-duty fuels. Large-volume production lines will reduce greenhouse gas (GHG) emission significantly but also low-volume chemicals are indispensable in building 'low-carbon' industries. The foreseeable feedstock change initiates innovation, securing societal wealth in the industrialized world and creating employment in regions producing biomass. When raising the investments in rerouting to sustainable biofuel and chemicals today competitiveness with fossil-based fuel and chemicals is a strong issue. Many countries adopted comprehensive bioeconomy strategies to tackle this challenge. These public actions are mostly biased to biofuel but should give well-balanced attention to renewable chemicals as well. PMID:26256682

  13. Metabolic engineering of microalgal based biofuel production: prospects and challenges

    Directory of Open Access Journals (Sweden)

    Chiranjib eBanerjee

    2016-03-01

    Full Text Available The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than other sources based on plant seeds. However, the greatest challenge in a transition to algae-based biofuel production is the various other complications involved in microalgal cultivation, its harvesting, concentration, drying and lipid extraction. Several green microalgae accumulate lipids, especially triacylglycerols (TAGs, which are main precursors in the production of lipid. The various aspects on metabolic pathway analysis of an oleaginous microalgae i.e. Chlamydomonas reinhardtii have elucidated some novel metabolically important genes and this enhances the lipid production in this microalgae. Adding to it, various other aspects in metabolic engineering using OptFlux and effectual bioprocess design also gives an interactive snapshot of enhancing lipid production which ultimately improvises the oil yield. This article reviews the current status of microalgal based technologies for biofuel production, bioreactor process design, flux analysis and it also provides various strategies to increase lipids accumulation via metabolic engineering.

  14. Metabolic Engineering of Microalgal Based Biofuel Production: Prospects and Challenges.

    Science.gov (United States)

    Banerjee, Chiranjib; Dubey, Kashyap K; Shukla, Pratyoosh

    2016-01-01

    The current scenario in renewable energy is focused on development of alternate and sustainable energy sources, amongst which microalgae stands as one of the promising feedstock for biofuel production. It is well known that microalgae generate much larger amounts of biofuels in a shorter time than other sources based on plant seeds. However, the greatest challenge in a transition to algae-based biofuel production is the various other complications involved in microalgal cultivation, its harvesting, concentration, drying and lipid extraction. Several green microalgae accumulate lipids, especially triacylglycerols (TAGs), which are main precursors in the production of lipid. The various aspects on metabolic pathway analysis of an oleaginous microalgae i.e., Chlamydomonas reinhardtii have elucidated some novel metabolically important genes and this enhances the lipid production in this microalgae. Adding to it, various other aspects in metabolic engineering using OptFlux and effectual bioprocess design also gives an interactive snapshot of enhancing lipid production which ultimately improvises the oil yield. This article reviews the current status of microalgal based technologies for biofuel production, bioreactor process design, flux analysis and it also provides various strategies to increase lipids accumulation via metabolic engineering. PMID:27065986

  15. The potential of C4 grasses for cellulosic biofuel production

    NARCIS (Netherlands)

    Weijde, van der R.T.; Alvim Kamei, C.L.; Torres Salvador, A.F.; Vermerris, W.; Dolstra, O.; Visser, R.G.F.; Trindade, L.M.

    2013-01-01

    With the advent of biorefinery technologies enabling plant biomass to be processed into biofuel, many researchers set out to study and improve candidate biomass crops. Many of these candidates are C4 grasses, characterized by a high productivity and resource use efficiency. In this review the potent

  16. Microwave-assisted pyrolysis of biomass for liquid biofuels production

    DEFF Research Database (Denmark)

    Yin, Chungen

    2012-01-01

    , is an attractive way for bio-oil production. Various efforts have been made to improve pyrolysis process towards higher yield and quality of liquid biofuels and better energy efficiency. Microwave-assisted pyrolysis is one of the promising attempts, mainly due to efficient heating of feedstock by ‘‘microwave...

  17. System studies on biofuel production via integrated biomass gasification

    OpenAIRE

    Andersson, Jim; Lundgren, Joakim; Malek, Laura; Hultegren, Christian; Pettersson, Karin; Wetterlund, Elisabeth

    2013-01-01

    A large number of national and international techno-economic studies on industrially integrated gasifiers for production of biofuels have been published during the recent years. These studies comprise different types of gasifiers (fluidized bed, indirect and entrained flow) integrated in different industries for the production of various types of chemicals and transportation fuels (SNG, FT-products, methanol, DME etc.) The results are often used for techno-economic comparisons between differe...

  18. Energy security for India: Biofuels, energy efficiency and food productivity

    International Nuclear Information System (INIS)

    The emergence of biofuel as a renewable energy source offers opportunities for significant climate change mitigation and greater energy independence to many countries. At the same time, biofuel represents the possibility of substitution between energy and food. For developing countries like India, which imports over 75% of its crude oil, fossil fuels pose two risks—global warming pollution and long-term risk that oil prices will undermine real living standards. This paper examines India's options for managing energy price risk in three ways: biofuel development, energy efficiency promotion, and food productivity improvements. Our salient results suggest that biodiesel shows promise as a transport fuel substitute that can be produced in ways that fully utilize marginal agricultural resources and hence promote rural livelihoods. First-generation bioethanol, by contrast, appears to have a limited ability to offset the impacts of oil price hikes. Combining the biodiesel expansion policy with energy efficiency improvements and food productivity increases proved to be a more effective strategy to enhance both energy and food security, help mitigate climate change, and cushion the economy against oil price shocks. - Highlights: • We investigate the role of biofuels in India applying a CGE model. • Biodiesel enhances energy security and improve rural livelihoods. • Sugarcane ethanol does not show positive impact on the economy. • Biodiesel and energy efficiency improvements together provide better results. • Food productivity further enhances biodiesel, and energy efficiency impacts

  19. Hydrocracking of used cooking oil for biofuels production.

    Science.gov (United States)

    Bezergianni, Stella; Kalogianni, Aggeliki

    2009-09-01

    Hydrocracking of used cooking oil is studied as a potential process for biofuels production. In this work several parameters are considered for evaluating the effectiveness of this technology, including hydrocracking temperature, liquid hourly space velocity (LHSV) and days on stream (DOS). Conversion and total biofuels production is favored by increasing temperature and decreasing LHSV. However moderate reaction temperatures and LHSVs are more attractive for diesel production, whereas higher temperatures and smaller LHSVs are more suitable for gasoline production. Furthermore heteroatom (S, N and O) removal increases as hydrocracking temperature increases, with de-oxygenation being particularly favorable. Saturation, however, is not favored with temperature indicating the necessity of a pre-treatment step prior to hydrocracking to enable saturation of the double bonds and heteroatom removal. Finally the impact of extended operation (catalyst life) on product yields and qualities indicates that all reactions are affected yet at different rates. PMID:19369071

  20. Modeling Regional Groundwater Implications of Biofuel Crop Production in the Great Lakes Region

    Science.gov (United States)

    Parish, A.; Kendall, A. D.; Basso, B.; Hyndman, D. W.

    2013-12-01

    In response to a growing call for renewable sources of energy that do not compete directly with food resources, the use of second-generation 'cellulosic' biofuel feedstocks has gained much attention in recent years. The push to advance the technologies that would make such a transformation possible is motivated by the United States Renewable Fuel Standard mandate to produce 36 billion gallons of biofuels by 2022, an increase of 334 percent from 2009. Many different crops, including maize, miscanthus, switchgrass, and poplar have shown promise as cellulosic feedstocks, and in an attempt to supply the needed biomass to meet the 2022 mandate, production of these crops have been on the rise. Yet little is known about the sustainability of large-scale conversion of land to cellulosic biofuel crop production; more research is needed to understand the effects that these crops will have on the quality and quantity of groundwater. This study presents a model scale-up approach to address three questions: What are the hydrologic and nutrient demands of the primary biofuel crops? Which biofuel crops are more water efficient in terms of demand verses energy produced? What are the types and availabilities of land to expand production of these biofuel crops? To answer these questions, we apply a point-based crop dynamics model in combination with a regional-scale hydrologic model, parameterized using stream discharge and chemistry data collected from two representative watersheds in Wisconsin. Approximately 17 stream sites in each watershed are selected for data collection for model parameterization, including stream discharge, nutrient concentrations, and basic chemical characteristics. We then use the System Approach to Land Use Sustainability (SALUS) model, which predicts crop growth under varying soil and climate conditions, to drive vegetation dynamics and groundwater transport of nutrients within the Integrated Landscape Hydrology Model (ILHM). ILHM predictions of stream

  1. Development of biological platform for the autotrophic production of biofuels

    Science.gov (United States)

    Khan, Nymul

    The research described herein is aimed at developing an advanced biofuel platform that has the potential to surpass the natural rate of solar energy capture and CO2 fixation. The underlying concept is to use the electricity from a renewable source, such as wind or solar, to capture CO 2 via a biological agent, such as a microbe, into liquid fuels that can be used for the transportation sector. In addition to being renewable, the higher rate of energy capture by photovoltaic cells than natural photosynthesis is expected to facilitate higher rate of liquid fuel production than traditional biofuel processes. The envisioned platform is part of ARPA-E's (Advanced Research Projects Agency - Energy) Electrofuels initiative which aims at supplementing the country's petroleum based fuel production with renewable liquid fuels that can integrate easily with the existing refining and distribution infrastructure (http://arpae. energy.gov/ProgramsProjects/Electrofuels.aspx). The Electrofuels initiative aimed to develop liquid biofuels that avoid the issues encountered in the current generation of biofuels: (1) the reliance of biomass-derived technologies on the inefficient process of photosynthesis, (2) the relatively energy- and resource-intensive nature of agronomic processes, and (3) the occupation of large areas of arable land for feedstock production. The process proceeds by the capture of solar energy into electrical energy via photovoltaic cells, using the generated electricity to split water into molecular hydrogen (H2) and oxygen (O2), and feeding these gases, along with carbon dioxide (CO2) emitted from point sources such as a biomass or coal-fired power plant, to a microbial bioprocessing platform. The proposed microbial bioprocessing platform leverages a chemolithoautotrophic microorganism (Rhodobacter capsulatus or Ralstonia eutropha) naturally able to utilize these gases as growth substrates, and genetically modified to produce a triterpene hydrocarbon fuel

  2. Assessment of environmental stresses for enhanced microalgal biofuel production-an overview

    Directory of Open Access Journals (Sweden)

    Dan eCheng

    2014-07-01

    Full Text Available Microalgal biofuels are currently considered to be the most promising alternative to future renewable energy source. Microalgae have great potential to produce various biofuels, including biodiesel, bioethanol, biomethane, and biohydrogen. Cultivation of biofuel-producing microalgae demands favorable environmental conditions, such as suitable light, temperature, nutrients, salinity, and pH. However, these conditions are not always compatible with the conditions beneficial to biofuel production, because biofuel-related compounds (such as lipids and carbohydrates tend to accumulate under environmental-stress conditions of light, temperature, nutrient, and salt. This paper presents a brief overview of the effects of environmental conditions on production of microalgal biomass and biofuel, with specific emphasis on how to utilize environmental stresses to improve biofuel productivity. The potential avenues of reaping the benefits of enhanced biofuel production by environmental stresses while maintaining high yields of biomass production have been discussed.

  3. Dynamic Modeling of Learning in Emerging Energy Industries: The Example of Advanced Biofuels in the United States

    Energy Technology Data Exchange (ETDEWEB)

    Vimmerstedt, Laura; Peterson, Steve; Bush, Brian

    2016-05-01

    This paper (and its supplemental model) presents novel approaches to modeling interactions and related policies among investment, production, and learning in an emerging competitive industry. New biomass-to-biofuels pathways are being developed and commercialized to support goals for U.S. advanced biofuel use, such as those in the Energy Independence and Security Act of 2007. We explore the impact of learning rates and techno-economics in a learning model excerpted from the Biomass Scenario Model (BSM), developed by the U.S. Department of Energy and the National Renewable Energy Laboratory to explore the impact of biofuel policy on the evolution of the biofuels industry. The BSM integrates investment, production, and learning among competing biofuel conversion options that are at different stages of industrial development. We explain the novel methods used to simulate the impact of differing assumptions about mature industry techno-economics and about learning rates while accounting for the different maturity levels of various conversion pathways. A sensitivity study shows that the parameters studied (fixed capital investment, process yield, progress ratios, and pre-commercial investment) exhibit highly interactive effects, and the system, as modeled, tends toward market dominance of a single pathway due to competition and learning dynamics.

  4. Dynamic Modeling of Learning in Emerging Energy Industries: The Example of Advanced Biofuels in the United States: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Vimmerstedt, Laura J.; Bush, Brian W.; Peterson, Steven O.

    2015-09-03

    This paper (and its supplemental model) presents novel approaches to modeling interactions and related policies among investment, production, and learning in an emerging competitive industry. New biomass-to-biofuels pathways are being developed and commercialized to support goals for U.S. advanced biofuel use, such as those in the Energy Independence and Security Act of 2007. We explore the impact of learning rates and techno-economics in a learning model excerpted from the Biomass Scenario Model (BSM), developed by the U.S. Department of Energy and the National Renewable Energy Laboratory to explore the impact of biofuel policy on the evolution of the biofuels industry. The BSM integrates investment, production, and learning among competing biofuel conversion options that are at different stages of industrial development. We explain the novel methods used to simulate the impact of differing assumptions about mature industry techno-economics and about learning rates while accounting for the different maturity levels of various conversion pathways. A sensitivity study shows that the parameters studied (fixed capital investment, process yield, progress ratios, and pre-commercial investment) exhibit highly interactive effects, and the system, as modeled, tends toward market dominance of a single pathway due to competition and learning dynamics.

  5. Microalgal cultivation with biogas slurry for biofuel production.

    Science.gov (United States)

    Zhu, Liandong; Yan, Cheng; Li, Zhaohua

    2016-11-01

    Microalgal growth requires a substantial amount of chemical fertilizers. An alternative to the utilization of fertilizer is to apply biogas slurry produced through anaerobic digestion to cultivate microalgae for the production of biofuels. Plenty of studies have suggested that anaerobic digestate containing high nutrient contents is a potentially feasible nutrient source to culture microalgae. However, current literature indicates a lack of review available regarding microalgal cultivation with biogas slurry for the production of biofuels. To help fill this gap, this review highlights the integration of digestate nutrient management with microalgal production. It first unveils the current status of microalgal production, providing basic background to the topic. Subsequently, microalgal cultivation technologies using biogas slurry are discussed in detail. A scale-up scheme for simultaneous biogas upgrade and digestate application through microalgal cultivation is then proposed. Afterwards, several uncertainties that might affect this practice are explored. Finally, concluding remarks are put forward.

  6. Synthetic feedback loop model for increasing microbial biofuel production using a biosensor

    Directory of Open Access Journals (Sweden)

    Mary eHarrison

    2012-10-01

    Full Text Available Current biofuel production methods use engineered bacteria to break down cellulose and convert it to biofuel. A major challenge in microbial fuel production is that increasing biofuel yields can be limited by the toxicity of the biofuel to the organism that is producing it. Previous research has demonstrated that efflux pumps are effective at increasing tolerance to various biofuels. However, when overexpressed, efflux pumps burden cells, which hinders growth and slows biofuel production. Therefore, the toxicity of the biofuel must be balanced with the toxicity of pump overexpression. We have developed a mathematical model for cell growth and biofuel production that implements a synthetic feedback loop using a biosensor to control efflux pump expression. In this way, the production rate will be maximal when the concentration of biofuel is low because the cell does not expend energy expressing efflux pumps when they are not needed. Additionally, the microbe is able to adapt to toxic conditions by triggering the expression of efflux pumps, which allow it to continue biofuel production. Sensitivity analysis indicates that the feedback sensor model is insensitive to most system parameters, but a few key parameters can influence growth and production. In comparison to systems that express efflux pumps at a constant level, the feedback sensor increases overall biofuel production by delaying pump expression until it is needed. This result is more pronounced when model parameters are variable because the system can use feedback to adjust to the actual rate of biofuel production.

  7. Tradeoffs and synergies between biofuel production and large-scale solar infrastructure in deserts

    Science.gov (United States)

    Ravi, S.; Lobell, D. B.; Field, C. B.

    2012-12-01

    Solar energy installations in deserts are on the rise, fueled by technological advances and policy changes. Deserts, with a combination of high solar radiation and availability of large areas unusable for crop production are ideal locations for large scale solar installations. For efficient power generation, solar infrastructures require large amounts of water for operation (mostly for cleaning panels and dust suppression), leading to significant moisture additions to desert soil. A pertinent question is how to use the moisture inputs for sustainable agriculture/biofuel production. We investigated the water requirements for large solar infrastructures in North American deserts and explored the possibilities for integrating biofuel production with solar infrastructure. In co-located systems the possible decline in yields due to shading by solar panels may be offsetted by the benefits of periodic water addition to biofuel crops, simpler dust management and more efficient power generation in solar installations, and decreased impacts on natural habitats and scarce resources in deserts. In particular, we evaluated the potential to integrate solar infrastructure with biomass feedstocks that grow in arid and semi-arid lands (Agave Spp), which are found to produce high yields with minimal water inputs. To this end, we conducted detailed life cycle analysis for these coupled agave biofuel - solar energy systems to explore the tradeoffs and synergies, in the context of energy input-output, water use and carbon emissions.

  8. Microalgae as a raw material for biofuels production.

    Science.gov (United States)

    Gouveia, Luisa; Oliveira, Ana Cristina

    2009-02-01

    Biofuels demand is unquestionable in order to reduce gaseous emissions (fossil CO(2), nitrogen and sulfur oxides) and their purported greenhouse, climatic changes and global warming effects, to face the frequent oil supply crises, as a way to help non-fossil fuel producer countries to reduce energy dependence, contributing to security of supply, promoting environmental sustainability and meeting the EU target of at least of 10% biofuels in the transport sector by 2020. Biodiesel is usually produced from oleaginous crops, such as rapeseed, soybean, sunflower and palm. However, the use of microalgae can be a suitable alternative feedstock for next generation biofuels because certain species contain high amounts of oil, which could be extracted, processed and refined into transportation fuels, using currently available technology; they have fast growth rate, permit the use of non-arable land and non-potable water, use far less water and do not displace food crops cultures; their production is not seasonal and they can be harvested daily. The screening of microalgae (Chlorella vulgaris, Spirulina maxima, Nannochloropsis sp., Neochloris oleabundans, Scenedesmus obliquus and Dunaliella tertiolecta) was done in order to choose the best one(s), in terms of quantity and quality as oil source for biofuel production. Neochloris oleabundans (fresh water microalga) and Nannochloropsis sp. (marine microalga) proved to be suitable as raw materials for biofuel production, due to their high oil content (29.0 and 28.7%, respectively). Both microalgae, when grown under nitrogen shortage, show a great increase (approximately 50%) in oil quantity. If the purpose is to produce biodiesel only from one species, Scenedesmus obliquus presents the most adequate fatty acid profile, namely in terms of linolenic and other polyunsaturated fatty acids. However, the microalgae Neochloris oleabundans, Nannochloropsis sp. and Dunaliella tertiolecta can also be used if associated with other

  9. Technology Roadmaps: Biofuels for Transport

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-07-01

    Biofuels could provide up to 27% of total transport fuel worldwide by 2050. The use of transport fuels from biomass, when produced sustainably, can help cut petroleum use and reduce CO2 emissions in the transport sector, especially in heavy transport. Sustainable biofuel technologies, in particular advanced biofuels, will play an important role in achieving this roadmap vision. The roadmap describes the steps necessary to realise this ambitious biofuels target; identifies key actions by different stakeholders, and the role for government policy to adopt measures needed to ensure the sustainable expansion of both conventional and advanced biofuel production.

  10. A Brief Literature Overview of Various Routes to Biorenewable Fuels from Lipids for the National Alliance for Advanced Biofuels and Bio-products (NAABB) Consortium

    Energy Technology Data Exchange (ETDEWEB)

    Albrecht, Karl O.; Hallen, Richard T.

    2011-03-29

    Renewable methods of producing transportation fuels are currently the focus of numerous large research efforts across the globe. Renewable fuel produced from algal lipids is one aspect of this research that could have profound implications on future transportation fuel requirements. However, technical challenges remain in several areas of algal-lipid-based fuels. These challenges include the identification and development of robust and productive algal species as well as extraction methods to recover the produced lipids. Not the least of these technical challenges is the conversion of the algae lipids to fungible fuels. This brief literature review focuses primarily on state-of-the-art “downstream” applications of producing fuel from fats and lipids, which can be applied to ongoing research with algae-derived lipids.

  11. The economic prospects of cellulosic biomass for biofuel production

    Science.gov (United States)

    Kumarappan, Subbu

    Alternative fuels for transportation have become the focus of intense policy debate and legislative action due to volatile oil prices, an unstable political environment in many major oil producing regions, increasing global demand, dwindling reserves of low-cost oil, and concerns over global warming. A major potential source of alternative fuels is biofuels produced from cellulosic biomass, which have a number of potential benefits. Recognizing these potential advantages, the Energy Independence and Security Act of 2007 has mandated 21 billion gallons of cellulosic/advanced biofuels per year by 2022. The United States needs 220-300 million tons of cellulosic biomass per year from the major sources such as agricultural residues, forestry and mill residues, herbaceous resources, and waste materials (supported by Biomass Crop Assistance Program) to meet these biofuel targets. My research addresses three key major questions concerning cellulosic biomass supply. The first paper analyzes cellulosic biomass availability in the United States and Canada. The estimated supply curves show that, at a price of 100 per ton, about 568 million metric tons of biomass is available in the United States, while 123 million metric tons is available in Canada. In fact, the 300 million tons of biomass required to meet EISA mandates can be supplied at a price of 50 per metric ton or lower. The second paper evaluates the farmers' perspective in growing new energy crops, such as switchgrass and miscanthus, in prime cropland, in pasture areas, or on marginal lands. My analysis evaluates how the farmers' returns from energy crops compare with those from other field crops and other agricultural land uses. The results suggest that perennial energy crops yielding at least 10 tons per acre annually will be competitive with a traditional corn-soybean rotation if crude oil prices are high (ranging from 88-178 per barrel over 2010-2019). If crude oil prices are low, then energy crops will not be

  12. Continuous production of palm biofuel under supercritical ethyl acetate

    International Nuclear Information System (INIS)

    Highlights: • Continuous synthesized biofuel from palm oil in supercritical ethyl acetate was examined. • Mass flow rate of palm oil and ethyl acetate mixture influent to biofuel production in continuous system. • Water addition to reacting mixture improves the production of fatty acid ethyl esters and triacetin. • The generated acetic acid from ETA hydrolysis can protect the products from thermal decomposition. - Abstract: The interesterification of palm oil in supercritical ethyl acetate (ETA) to produce fatty acid ethyl ester (FAEEs) or biofuel was conducted in a continuous tubular reactor. The density of the mixtures in the system was estimated using the Peng–Robinson equation of state process simulator, and the residence time was calculated. The effects of the reaction conditions, including the molar ratios of palm oil to ethyl acetate, the temperature, and the pressure, were investigated under various mass flow rates of the mixtures and optimized. The results showed that reaction temperatures above 653 K and long residence times affected the content of FAEEs and triacetin, a valuable by-product. The addition of water to the mixture in a 1:30:10 M ratio of palm oil to ethyl acetate to water at 653 K, 16 MPa, and a mixture mass flow rate of 1.5 g/min increased the total production of FAEEs and triacetin from 90.9 to 101.5 wt% in 42.4 min. The main finding of the present study is that triglyceride associated with ETA hydrolysis used to form acetic acid protected the products from decomposition at high temperatures and long residence times. The results will aid the selection of an efficient and economical process for alternative biofuel production from palm oil in supercritical ETA

  13. Metabolic engineering for isoprenoid-based biofuel production.

    Science.gov (United States)

    Gupta, P; Phulara, S C

    2015-09-01

    Sustainable economic and industrial growth is the need of the hour and it requires renewable energy resources having better performance and compatibility with existing fuel infrastructure from biological routes. Isoprenoids (C ≥ 5) can be a potential alternative due to their diverse nature and physiochemical properties similar to that of petroleum based fuels. In the past decade, extensive research has been done to utilize metabolic engineering strategies in micro-organisms primarily, (i) to overcome the limitations associated with their natural and non-natural production and (ii) to develop commercially competent microbial strain for isoprenoid-based biofuel production. This review briefly describes the engineered isoprenoid biosynthetic pathways in well-characterized microbial systems for the production of several isoprenoid-based biofuels and fuel precursors. PMID:26095690

  14. PRODUCTION OF BIOFUELS WITH CORN: A WELFARE ANALYSIS IN MEXICO

    Directory of Open Access Journals (Sweden)

    Salvador González-Andrade

    2010-04-01

    Full Text Available According to the decrease in the global offer and the increase of prices of fossil fuels exists a growing trend to generate energy alternatives. The production of commodities to generate biofuels competes with the production of foods, fibers and wood. The ethanol production from grains as corn and weath will impact the global market of food. In the United States, the main supplier of yellow corn to Mexico, in 2012 is foreseen to use a mixture of biofuels with ten percent of ethanol (OECD, 2006. The use of corn, basic food of Mexicans, in the generation of ethanol will have redistributive effects of social welfare through prices and amounts produced and consumed.

  15. Methods and materials for deconstruction of biomass for biofuels production

    Energy Technology Data Exchange (ETDEWEB)

    Schoeniger, Joseph S; Hadi, Masood Zia

    2015-05-05

    The present invention relates to nucleic acids, peptides, vectors, cells, and plants useful in the production of biofuels. In certain embodiments, the invention relates to nucleic acid sequences and peptides from extremophile organisms, such as SSO1949 and Ce1A, that are useful for hydrolyzing plant cell wall materials. In further embodiments, the invention relates to modified versions of such sequences that have been optimized for production in one or both of monocot and dicot plants. In other embodiments, the invention provides for targeting peptide production or activity to a certain location within the cell or organism, such as the apoplast. In further embodiments, the invention relates to transformed cells or plants. In additional embodiments, the invention relates to methods of producing biofuel utilizing such nucleic acids, peptides, targeting sequences, vectors, cells, and/or plants.

  16. Perspectives on the use of transcriptomics to advance biofuels

    Directory of Open Access Journals (Sweden)

    Siseon Lee

    2015-11-01

    Full Text Available As a field within the energy research sector, bioenergy is continuously expanding. Although much has been achieved and the yields of both ethanol and butanol have been improved, many avenues of research to further increase these yields still remain. This review covers current research related with transcriptomics and the application of this high-throughput analytical tool to engineer both microbes and plants with the penultimate goal being better biofuel production and yields. The initial focus is given to the responses of fermentative microbes during the fermentative production of acids, such as butyric acid, and solvents, including ethanol and butanol. As plants offer the greatest natural renewable source of fermentable sugars within the form of lignocellulose, the second focus area is the transcriptional responses of microbes when exposed to plant hydrolysates and lignin-related compounds. This is of particular importance as the acid/base hydrolysis methods commonly employed to make the plant-based cellulose available for enzymatic hydrolysis to sugars also generates significant amounts of lignin-derivatives that are inhibitory to fermentative bacteria and microbes. The article then transitions to transcriptional analyses of lignin-degrading organisms, such as Phanerochaete chrysosporium, as an alternative to acid/base hydrolysis. The final portion of this article will discuss recent transcriptome analyses of plants and, in particular, the genes involved in lignin production. The rationale behind these studies is to eventually reduce the lignin content present within these plants and, consequently, the amount of inhibitors generated during the acid/base hydrolysis of the lignocelluloses. All four of these topics represent key areas where transcriptomic research is currently being conducted to identify microbial genes and their responses to products and inhibitors as well as those related with lignin degradation/formation.

  17. Coupling greenhouse gas credits with biofuel production cost in determining conversion plant size

    Energy Technology Data Exchange (ETDEWEB)

    Gan, Jianbang (Texas A and M Univ., Dept. of Ecosystem Science and Management (United States)), email: j-gan@tamu.edu; Smith, C.T. (Univ. of Toronto, Faculty of Forestry (Canada))

    2010-07-01

    Biofuel plant size is one of the key variables in biofuel supply chain analysis as it plays a pivotal role in controlling the efficacy of both feedstock supply and feedstock-to-biofuel conversion. The unit production cost and greenhouse gas (GHG) balance of biofuels vary with plant size. We develop an analytical framework for integrating biofuel production costs and GHG balance derived from life-cycle analysis into supply chain optimization, followed by its application to ethanol production using forest biomass in the southern United States. We derive formulas for determining the optimal biofuel plant size and the corresponding feedstock supply radius based on the minimization of biofuel production costs less GHG benefits. Our results indicate that though biofuel plant size and feedstock supply radius should be augmented by considering GHG benefits, the GHG price will have a more significant impact on net biofuel production costs than on conversion plant size or feedstock supply radius. With a rise in the GHG price the net biofuel production cost tends to increase while the directions of change in plant size and feedstock supply radius are uncertain, depending upon the costs and GHG emissions of biomass transport and feedstock-to-fuel conversion. Combining GHG offset values with biofuel production costs enables us to more holistically examine the biofuel supply chain. (orig.)

  18. Optimal localisation of next generation Biofuel production in Sweden

    Energy Technology Data Exchange (ETDEWEB)

    Wetterlund, Elisabeth [Linkoeping Univ., Linkoeping (Sweden); Pettersson, Karin [Chalmers Univ. of Technology, Goeteborg (Sweden); Mossberg, Johanna [SP Technical Research Inst. of Sweden, Boraas (Sweden)] [and others

    2013-09-01

    With a high availability of lignocellulosic biomass and various types of cellulosic by-products, as well as a large number of industries, Sweden is a country of great interest for future large scale production of sustainable, next generation biofuels. This is most likely also a necessity as Sweden has the ambition to be independent of fossil fuels in the transport sector by the year 2030 and completely fossil free by 2050. In order to reach competitive biofuel production costs, plants with large production capacities are likely to be required. Feedstock intake capacities in the range of about 1-2 million tonnes per year, corresponding to a biomass feed of 300-600 MW, can be expected, which may lead to major logistical challenges. To enable expansion of biofuel production in such large plants, as well as provide for associated distribution requirements, it is clear that substantial infrastructure planning will be needed. The geographical location of the production plant facilities is therefore of crucial importance and must be strategic to minimise the transports of raw material as well as of final product. Competition for the available feedstock, from for example forest industries and CHP plants (combined heat and power) further complicates the localisation problem. Since the potential for an increased biomass utilisation is limited, high overall resource efficiency is of great importance. Integration of biofuel production processes in existing industries or in district heating systems may be beneficial from several aspects, such as opportunities for efficient heat integration, feedstock and equipment integration, as well as access to existing experience and know-how. This report describes the development of Be Where Sweden, a geographically explicit optimisation model for localisation of next generation biofuel production plants in Sweden. The main objective of developing such a model is to be able to assess production plant locations that are robust to varying

  19. Land substitution effects of biofuel side products and implications on the land area requirement for EU 2020 biofuel targets

    Energy Technology Data Exchange (ETDEWEB)

    Oezdemir, Enver Doruk; Haerdtlein, Marlies; Eltrop, Ludger [University of Stuttgart, Institute of Energy Economics and the Rational Use of Energy, Hessbruehlstr. 49a, 70565 Stuttgart (Germany)

    2009-08-15

    The provision of biofuels today is based on energy crops rather than residual biomass, which results in the requirement of agricultural land area. The side products may serve as animal feed and thus prevent cultivation of other feedstock and the use of corresponding land area. These effects of biofuel provision have to be taken into account for a comprising assessment of land area requirement for biofuel provision. Between 18.5 and 21.1 Mio. hectares (ha) of land area is needed to meet the EU 2020 biofuel target depending on the biofuel portfolio when substitution effects are neglected. The utilization of the bioethanol side products distiller's dried grain and solubles (DDGS) and pressed beet slices may save up to 0.7 Mio. ha of maize cultivation area in the EU. The substitution effect due to the utilization of biodiesel side products (oil cakes of rape, palm and soy) as animal feed may account for up to 7.1 Mio. ha of soy cultivation area in Brazil. The results show that the substitution of land area due to use of side products might ease the pressures on land area requirement considerably and should therefore not be neglected in assessing the impacts of biofuel provision worldwide. (author)

  20. Land substitution effects of biofuel side products and implications on the land area requirement for EU 2020 biofuel targets

    Energy Technology Data Exchange (ETDEWEB)

    Ozdemir, Enver Doruk [University of Stuttgart, Institute of Energy Economics and the Rational Use of Energy, Hessbruehlstr. 49a, 70565 Stuttgart (Germany)], E-mail: do@ier.uni-stuttgart.de; Haerdtlein, Marlies; Eltrop, Ludger [University of Stuttgart, Institute of Energy Economics and the Rational Use of Energy, Hessbruehlstr. 49a, 70565 Stuttgart (Germany)

    2009-08-15

    The provision of biofuels today is based on energy crops rather than residual biomass, which results in the requirement of agricultural land area. The side products may serve as animal feed and thus prevent cultivation of other feedstock and the use of corresponding land area. These effects of biofuel provision have to be taken into account for a comprising assessment of land area requirement for biofuel provision. Between 18.5 and 21.1 Mio. hectares (ha) of land area is needed to meet the EU 2020 biofuel target depending on the biofuel portfolio when substitution effects are neglected. The utilization of the bioethanol side products distiller's dried grain and solubles (DDGS) and pressed beet slices may save up to 0.7 Mio. ha of maize cultivation area in the EU. The substitution effect due to the utilization of biodiesel side products (oil cakes of rape, palm and soy) as animal feed may account for up to 7.1 Mio. ha of soy cultivation area in Brazil. The results show that the substitution of land area due to use of side products might ease the pressures on land area requirement considerably and should therefore not be neglected in assessing the impacts of biofuel provision worldwide.

  1. Land substitution effects of biofuel side products and implications on the land area requirement for EU 2020 biofuel targets

    International Nuclear Information System (INIS)

    The provision of biofuels today is based on energy crops rather than residual biomass, which results in the requirement of agricultural land area. The side products may serve as animal feed and thus prevent cultivation of other feedstock and the use of corresponding land area. These effects of biofuel provision have to be taken into account for a comprising assessment of land area requirement for biofuel provision. Between 18.5 and 21.1 Mio. hectares (ha) of land area is needed to meet the EU 2020 biofuel target depending on the biofuel portfolio when substitution effects are neglected. The utilization of the bioethanol side products distiller's dried grain and solubles (DDGS) and pressed beet slices may save up to 0.7 Mio. ha of maize cultivation area in the EU. The substitution effect due to the utilization of biodiesel side products (oil cakes of rape, palm and soy) as animal feed may account for up to 7.1 Mio. ha of soy cultivation area in Brazil. The results show that the substitution of land area due to use of side products might ease the pressures on land area requirement considerably and should therefore not be neglected in assessing the impacts of biofuel provision worldwide.

  2. Biofuel production from plant biomass derived sugars

    Energy Technology Data Exchange (ETDEWEB)

    Cripps, R.

    2007-03-15

    This report details the results of a project that aimed to develop a recombinant thermophilic microorganism able to produce ethanol in a commercial yield from mixed C5 (xylose and arabinose) and C6 (mainly glucose) sugar substrates typically found in biomass hydrolysates. The main focus of the project was on producing a stable recombinant which formed ethanol as its major product and did not produce significant quantities of by-products. The costs of bioethanol could be substantially reduced if cheap plant-based feedstocks could be utilised. This study focussed on a strain of Geobacillus thermoglucosidasius known to be a thermophilic ethanol producer and developed the genetic manipulation techniques necessary to engineer its metabolism such that unwanted products (mainly organic acids) were no longer formed and ethanol became the overwhelming product. An appropriate genetic took kit to allow the required metabolic engineering was acquired and used to inactivate the genes of the metabolic pathways involved in the formation of the organic acids (e.g. lactic acid) and to up-regulate genes concerned with the formation of ethanol. This allowed the flow of metabolites derived from the sugar substrates to be redirected to the desired product. Stable mutants lacking the ability to form lactic acid were created and shown to give enhanced levels of ethanol, with yields from glucose approaching those achieved in yeast fermentations and low by-product formation.

  3. Biofuels and climate neutrality - system analysis of production and utilisation

    International Nuclear Information System (INIS)

    The objectives of this study were to investigate to what extent biofuels can be said to be climate neutral. An assessment of greenhouse gas emissions from the production and utilisation chains of a number of solid biofuels were made based on data available in the literature. The data has been used for making radiative forcing calculations. The study also includes a comparison between imported and domestic solid biofuels. We conclude that none of the investigated biofuel chains are 'climate neutral', since all of them result in net emissions of greenhouse gases. However, all of the chains result in lower emissions than corresponding emissions from the use of fossil fuels. The emission estimates for the fuel chains varies depending on fuels and on how system boundaries have been set in the different studies. The following factors can contribute significantly to the total emissions of greenhouse gases of the production and utilisation chain of a biofuel: impact of production system on soil carbon storage, land use methods (especially use of drained peatlands), the use of fertilisers (both direct and indirect), combustion technology, refining of the fuel (i.e. pelletisation) and storage (especially of comminuted fuels). Other sources that also contribute to the emissions during a production and utilisation chain are; harvesting machines, transportation and waste handling. The climate impacts of the greenhouse gas emissions from one of the biofuels, i.e. forest residues, were compared to the impacts of fossil fuels by the concept of radiative forcing. In the radiative forcing calculations the CO2 emissions from combustion of biofuels and the CO2 emissions that would have occurred if the residues had been left in the forest to decompose were included, and their different dynamics taken into consideration. The decomposition results in CO2 emissions during a long time period and in an amount equalling those that are emitted during combustion. Only a minor part is due to

  4. Reclaimed Water Use in Biofuel Production

    Directory of Open Access Journals (Sweden)

    María del Pino Palacios-Diaz

    2015-09-01

    Full Text Available Jatropha curcas L., a toxic species that does not interfere with the food chain, produces biodiesel of better environmental quality than mineral oils. However, in order to cultivate it sustainably, it is necessary to optimize the limited resources used, mainly water and soil. Therefore, in arid areas, it is necessary to cultivate under intensive conditions, irrigate with reclaimed water and cut production costs. To optimize water consumption, partial root-zone drying (PRD, which keeps a part of the root system dry, was used. This water management strategy, employed successfully in other oil crops, yielded less fruit per bunch, but more fruit bunches per plant. This fact will probably allow to establish higher planting density and, consequently, higher productivity per surface unit. This is one of the few available options for improving profitability as production per tree is stable (1.25 kg seed plant−1 year−1 for the most productive trees, with excellent climate and soil, and no limitations water use. A high percentage of fruit lying on the ground (24% and non-uniform timing in fruit production (except some specimens greatly hinder its mechanization. Although this crop’s environmental and socio-economic benefits are not taken into account, it is very difficult, with only the calculated water consumption (15.5 m3 water per L of oil or 5.6 m3 water per L of oil according to our best estimations, to consider it a profitable option.

  5. Efficient production of automotive biofuels; Effektiv produktion av biodrivmedel

    Energy Technology Data Exchange (ETDEWEB)

    Gode, Jenny; Hagberg, Linus; Rydberg, Tomas; Raadberg, Henrik; Saernholm, Erik

    2008-07-01

    The report describes opportunities and consequences associated with biomass polygeneration plants, in particular the role that heat plants (HP) or combined heat and power plants (CHP) in district heating systems can play in the production of automotive biofuels. The aim of the report is to provide a knowledge base to stakeholders to help assess energy and environmental benefits associated with collaborative approaches in planning, constructing and operating energy plants. Several configurations are possible for an energy polygeneration plant, but this report focuses on configurations in which a plant for automotive biofuel production and a district heating system with HPs or CHPs have been integrated in some way in order to achieve added value. The modes of integration are several, e.g.: - Supply of process steam from the CHP to the fuel plant, by which the time of operation for the CHP can be extended; Supply of surplus heat from the fuel plant to the district heating system; Material exchange between the systems, by use of residue streams from the fuel plant as fuel in the HP/CHP; Surplus heat from the fuel plant used for drying of the solid fuel to the HP/CHP or for drying of raw material for pellets production; Co-location providing opportunities for shared infrastructure for raw material handling, service systems, utilities and/or logistics. The report principally addresses integration options of the first three types, but describes briefly also pellets production. The starting point for the analysis of integration options is the description of technologies of interest for the production of automotive biofuels. Commercially available technologies are of prime interest, but also a couple of technologies under development are included in this part of the study. In addition to outlining the process characteristics for these processes, surrounding conditions and system requirements are briefly outlined. The results are summarized in Table S1. Ethanol fermentation

  6. Lipid-based biofuel production from wastewater

    OpenAIRE

    Muller, Emilie; Sheik, Abdul; Wilmes, Paul

    2014-01-01

    Increasing world population, urbanization and industrialization are driving global increases in wastewater production. Wastewater comprises significant amounts of chemical energy primarily in the form of organic molecules (in particular lipids), which are currently not being recovered comprehensively. Within biological wastewater treatment (BWWT) systems, specialized microorganisms assimilate and store lipids anaerobically. These intracellular stores represent interesting feedstocks for biofu...

  7. Liquid biofuel production from volatile fatty acids

    NARCIS (Netherlands)

    Steinbusch, K.J.J.

    2010-01-01

    The production of renewable fuels and chemicals reduces the dependency on fossil fuels and limits the increase of CO2 concentration in the atmosphere only if a sustainable feedstock and an energy efficient process are used. The thesis assesses the possibility to use municipal and industrial waste as

  8. Integrated microbial processes for biofuels and high value-added products: the way to improve the cost effectiveness of biofuel production.

    Science.gov (United States)

    da Silva, Teresa Lopes; Gouveia, Luísa; Reis, Alberto

    2014-02-01

    The production of microbial biofuels is currently under investigation, as they are alternative sources to fossil fuels, which are diminishing and their use has a negative impact on the environment. However, so far, biofuels derived from microbes are not economically competitive. One way to overcome this bottleneck is the use of microorganisms to transform substrates into biofuels and high value-added products, and simultaneously taking advantage of the various microbial biomass components to produce other products of interest, as an integrated process. In this way, it is possible to maximize the economic value of the whole process, with the desired reduction of the waste streams produced. It is expected that this integrated system makes the biofuel production economically sustainable and competitive in the near future. This review describes the investigation on integrated microbial processes (based on bacteria, yeast, and microalgal cultivations) that have been experimentally developed, highlighting the importance of this approach as a way to optimize microbial biofuel production process.

  9. Downgrading recent estimates of land available for biofuel production.

    Science.gov (United States)

    Fritz, Steffen; See, Linda; van der Velde, Marijn; Nalepa, Rachel A; Perger, Christoph; Schill, Christian; McCallum, Ian; Schepaschenko, Dmitry; Kraxner, Florian; Cai, Ximing; Zhang, Xiao; Ortner, Simone; Hazarika, Rubul; Cipriani, Anna; Di Bella, Carlos; Rabia, Ahmed H; Garcia, Alfredo; Vakolyuk, Mar'yana; Singha, Kuleswar; Beget, Maria E; Erasmi, Stefan; Albrecht, Franziska; Shaw, Brian; Obersteiner, Michael

    2013-02-01

    Recent estimates of additional land available for bioenergy production range from 320 to 1411 million ha. These estimates were generated from four scenarios regarding the types of land suitable for bioenergy production using coarse-resolution inputs of soil productivity, slope, climate, and land cover. In this paper, these maps of land availability were assessed using high-resolution satellite imagery. Samples from these maps were selected and crowdsourcing of Google Earth images was used to determine the type of land cover and the degree of human impact. Based on this sample, a set of rules was formulated to downward adjust the original estimates for each of the four scenarios that were previously used to generate the maps of land availability for bioenergy production. The adjusted land availability estimates range from 56 to 1035 million ha depending upon the scenario and the ruleset used when the sample is corrected for bias. Large forest areas not intended for biofuel production purposes were present in all scenarios. However, these numbers should not be considered as definitive estimates but should be used to highlight the uncertainty in attempting to quantify land availability for biofuel production when using coarse-resolution inputs with implications for further policy development. PMID:23308357

  10. Biofuel production system with operation flexibility: Evaluation of economic and environmental performance under external disturbance

    Science.gov (United States)

    Kou, Nannan

    Biomass derived liquid hydrocarbon fuel (biofuel) has been accepted as an effective way to mitigate the reliance on petroleum and reduce the greenhouse gas emissions. An increasing demand for second generation biofuels, produced from ligno-cellulosic feedstock and compatible with current infrastructure and vehicle technologies, addresses two major challenges faced by the current US transportation sector: energy security and global warming. However, biofuel production is subject to internal disturbances (feedstock supply and commodity market) and external factors (energy market). The biofuel industry has also heavily relied on government subsidy during the early development stages. In this dissertation, I investigate how to improve the economic and environmental performance of biorefineries (and biofuel plant), as well as enhance its survivability under the external disturbances. Three types of disturbance are considered: (1) energy market fluctuation, (2) subsidy policy uncertainty, and (3) extreme weather conditions. All three factors are basically volatile, dynamic, and even unpredictable, which makes them difficult to model and have been largely ignored to date. Instead, biofuel industry and biofuel research are intensively focused on improving feedstock conversion efficiency and capital cost efficiency while assuming these advancements alone will successfully generate higher profit and thus foster the biofuel industry. The collapse of the largest corn ethanol biofuel company, Verasun Energy, in 2008 calls into question this efficiency-driven approach. A detailed analysis has revealed that although the corn ethanol plants operated by Verasun adopted the more efficient (i.e. higher ethanol yield per bushel of corn and lower capital cost) dry-mill technology, they could not maintain a fair profit margin under fluctuating market condition which made ethanol production unprofitable. This is because dry-mill plant converts a single type of biomass feedstock (corn

  11. Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production.

    Science.gov (United States)

    Lin, Lu; Xu, Jian

    2013-11-01

    Interest in thermophilic bacteria as live-cell catalysts in biofuel and biochemical industry has surged in recent years, due to their tolerance of high temperature and wide spectrum of carbon-sources that include cellulose. However their direct employment as microbial cellular factories in the highly demanding industrial conditions has been hindered by uncompetitive biofuel productivity, relatively low tolerance to solvent and osmic stresses, and limitation in genome engineering tools. In this work we review recent advances in dissecting and engineering the metabolic and regulatory networks of thermophilic bacteria for improving the traits of key interest in biofuel industry: cellulose degradation, pentose-hexose co-utilization, and tolerance of thermal, osmotic, and solvent stresses. Moreover, new technologies enabling more efficient genetic engineering of thermophiles were discussed, such as improved electroporation, ultrasound-mediated DNA delivery, as well as thermo-stable plasmids and functional selection systems. Expanded applications of such technological advancements in thermophilic microbes promise to substantiate a synthetic biology perspective, where functional parts, module, chassis, cells and consortia were modularly designed and rationally assembled for the many missions at industry and nature that demand the extraordinary talents of these extremophiles.

  12. Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production.

    Science.gov (United States)

    Lin, Lu; Xu, Jian

    2013-11-01

    Interest in thermophilic bacteria as live-cell catalysts in biofuel and biochemical industry has surged in recent years, due to their tolerance of high temperature and wide spectrum of carbon-sources that include cellulose. However their direct employment as microbial cellular factories in the highly demanding industrial conditions has been hindered by uncompetitive biofuel productivity, relatively low tolerance to solvent and osmic stresses, and limitation in genome engineering tools. In this work we review recent advances in dissecting and engineering the metabolic and regulatory networks of thermophilic bacteria for improving the traits of key interest in biofuel industry: cellulose degradation, pentose-hexose co-utilization, and tolerance of thermal, osmotic, and solvent stresses. Moreover, new technologies enabling more efficient genetic engineering of thermophiles were discussed, such as improved electroporation, ultrasound-mediated DNA delivery, as well as thermo-stable plasmids and functional selection systems. Expanded applications of such technological advancements in thermophilic microbes promise to substantiate a synthetic biology perspective, where functional parts, module, chassis, cells and consortia were modularly designed and rationally assembled for the many missions at industry and nature that demand the extraordinary talents of these extremophiles. PMID:23510903

  13. Soil carbon sequestration or biofuel production: new land-use opportunities for mitigating climate over abandoned Soviet farmlands.

    Science.gov (United States)

    Vuichard, Nicolas; Ciais, Philippe; Wolf, Adam

    2009-11-15

    Although the CO(2) mitigation potential of biofuels has been studied by extrapolation of small-scale studies, few estimates exist of the net regional-scale carbon balance implications of biofuel cultivations programs, either growing conventional biofuel crops or applying new advanced technologies. Here we used a spatially distributed process-driven model over the 20 Mha of recently abandoned agricultural lands of the Former Soviet Union to quantify the GHG mitigation by biofuel production from Low Input/High Diversity (LIHD) grass-legume prairies and to compare this GHG mitigation with the one of soil C sequestration as it currently occurs. LIHD has recently received a lot of attention as an emerging opportunity to produce biofuels over marginal lands leading to a good energy efficiency with minimal adverse consequences on food security and ecosystem services. We found that, depending on the time horizon over which one seeks to maximize the GHG benefit, the optimal time for implementing biofuel production shifts from "never" (short-term horizon) to "as soon as possible" (longer-term horizon). These results highlight the importance of reaching agreement a priori on the target time interval during which biofuels are expected to play a role within the global energy system, to avoid deploying biofuel technology over a time interval for which it has a detrimental impact on the GHG mitigation objective. The window of opportunity for growing LIHD also stresses the need to reduce uncertainties in soil C inputs, turnover, and soil organic matter stability under current and future climate and management practices.

  14. An overview of algae biofuel production and potential environmental impact.

    Science.gov (United States)

    Menetrez, Marc Y

    2012-07-01

    Algae are among the most potentially significant sources of sustainable biofuels in the future of renewable energy. A feedstock with virtually unlimited applicability, algae can metabolize various waste streams (e.g., municipal wastewater, carbon dioxide from industrial flue gas) and produce products with a wide variety of compositions and uses. These products include lipids, which can be processed into biodiesel; carbohydrates, which can be processed into ethanol; and proteins, which can be used for human and animal consumption. Algae are commonly genetically engineered to allow for advantageous process modification or optimization. However, issues remain regarding human exposure to algae-derived toxins, allergens, and carcinogens from both existing and genetically modified organisms (GMOs), as well as the overall environmental impact of GMOs. A literature review was performed to highlight issues related to the growth and use of algal products for generating biofuels. Human exposure and environmental impact issues are identified and discussed, as well as current research and development activities of academic, commercial, and governmental groups. It is hoped that the ideas contained in this paper will increase environmental awareness of issues surrounding the production of algae and will help the algae industry develop to its full potential. PMID:22681590

  15. Identification Of Marginal Land Suitable For Biofuel Production In Serbia

    Directory of Open Access Journals (Sweden)

    Radojević Uroš

    2015-11-01

    Full Text Available The use of biomass as a potential energy source has both advantages and disadvantages. Biomass is a potential source of fuel energy that provides economic and environmental benefits such as less expensive and less energy intensive production, carbon sequestration and soil preservation. However, the main concern associated with biofuels is that land needed for food will be used for biofuel crops. One potential solution is the use of marginal lands which are not suited for food production. Marginal lands generally refer to the areas not only with low production, but also with limitations that make them unsuitable for agricultural practices and ecosystem functions. This can be due to various forms of land degradation such as pollution, surface exploitation of mineral resources, erosion, overexploitation and others. We used remotely sensed data, environmental data and field survey data to identify possible marginal lands in Serbia. All gathered data was transferred to GIS in order to create maps and database of potential marginal lands which could be used for biomass production.

  16. A model for improving microbial biofuel production using a synthetic feedback loop

    Energy Technology Data Exchange (ETDEWEB)

    Dunlop, Mary; Keasling, Jay; Mukhopadhyay, Aindrila

    2011-07-14

    Cells use feedback to implement a diverse range of regulatory functions. Building synthetic feedback control systems may yield insight into the roles that feedback can play in regulation since it can be introduced independently of native regulation, and alternative control architectures can be compared. We propose a model for microbial biofuel production where a synthetic control system is used to increase cell viability and biofuel yields. Although microbes can be engineered to produce biofuels, the fuels are often toxic to cell growth, creating a negative feedback loop that limits biofuel production. These toxic effects may be mitigated by expressing efflux pumps that export biofuel from the cell. We developed a model for cell growth and biofuel production and used it to compare several genetic control strategies for their ability to improve biofuel yields. We show that controlling efflux pump expression directly with a biofuel-responsive promoter is a straight forward way of improving biofuel production. In addition, a feed forward loop controller is shown to be versatile at dealing with uncertainty in biofuel production rates.

  17. Review on biofuel oil and gas production processes from microalgae

    International Nuclear Information System (INIS)

    Microalgae, as biomass, are a potential source of renewable energy, and they can be converted into energy such as biofuel oil and gas. This paper presents a brief review on the main conversion processes of microalgae becoming energy. Since microalgae have high water content, not all biomass energy conversion processes can be applied. By using thermochemical processes, oil and gas can be produced, and by using biochemical processes, ethanol and biodiesel can be produced. The properties of the microalgae product are almost similar to those of offish and vegetable oils, and therefore, it can be considered as a substitute of fossil oil.

  18. The impact of extreme drought on the biofuel feedstock production

    Science.gov (United States)

    hussain, M.; Zeri, M.; Bernacchi, C.

    2013-12-01

    Miscanthus (Miscanthus x giganteus) and Switchgrass (Panicum virgatum) have been identified as the primary targets for second-generation cellulosic biofuel crops. Prairie managed for biomass is also considered as one of the alternative to conventional biofuel and promised to provide ecosystem services, including carbon sequestration. These perennial grasses possess a number of traits that make them desirable biofuel crops and can be cultivated on marginal lands or interspersed with maize and soybean in the Corn Belt region. The U.S. Corn Belt region is the world's most productive and expansive maize-growing region, approximately 20% of the world's harvested corn hectares are found in 12 Corn Belt states. The introduction of a second generation cellulosic biofuels for biomass production in a landscape dominated by a grain crop (maize) has potential implications on the carbon and water cycles of the region. This issue is further intensified by the uncertainty in the response of the vegetation to the climate change induced drought periods, as was seen during the extreme droughts of 2011 and 2012 in the Midwest. The 2011 and 2012 growing seasons were considered driest since the 1932 dust bowl period; temperatures exceeded 3.0 °C above the 50- year mean and precipitation deficit reached 50 %. The major objective of this study was to evaluate the drought responses (2011 and 2012) of corn and perennial species at large scale, and to determine the seasonability of carbon and water fluxes in the response of controlling factors. We measured net CO2 ecosystem exchange (NEE) and water fluxes of maize-maize-soybean, and perennial species such as miscanthus, switchgrass and mixture of prairie grasses, using eddy covariance in the University of Illinois energy farm at Urbana, IL. The data presented here were for 5 years (2008- 2012). In the first two years, higher NEE in maize led to large CO2 sequestration. NEE however, decreased in dry years, particularly in 2012. On the other

  19. Spirogyra biomass a renewable source for biofuel (bioethanol Production

    Directory of Open Access Journals (Sweden)

    Fuad Salem Eshaq

    2010-12-01

    Full Text Available Biofuels refer to renewable fuels from biological sources that can be used for heat, electricity and fuel. The fuels obtained from algae are termed as third generation fuels. The production of fuel from algae provides many advantages when compared to the fuel produced from other sources like agrobased raw materials. Other than environmental pollution control the algal biofuel will help in reduction of the fuel cost when compared to the agrobased and fossil fuels. In the present study algae specifically Spirogyra was used for the production of bioethanol by the fermentative process. A comparative study was carried out by using chemically pre-treated anduntreated Spirogyra biomass. The Spirogyra has a very simple cell wall made up of cellulose and starch that can be converted to ethanol by the fermentation process. The Spirogyra biomass was subjected to saccharification process by the fungal organism Aspergillus niger MTCCC 2196 for the hydrolysis, this process was followed by the fermentation using yeast Saccharomyces cerevisiae MTCC170 for the production of alcohol. A high yield of ethanol was recorded for untreated Spirogyra biomass when compared to chemically pre-treated biomass. The yield of alcohol using algal biomass is more when compared to alcohol produced from other sources like agrobased rawmaterials.

  20. Recent advances on enzymatic glucose/oxygen and hydrogen/oxygen biofuel cells: Achievements and limitations

    Science.gov (United States)

    Cosnier, Serge; Gross, Andrew J.; Le Goff, Alan; Holzinger, Michael

    2016-09-01

    The possibility of producing electrical power from chemical energy with biological catalysts has induced the development of biofuel cells as viable energy sources for powering portable and implanted electronic devices. These power sources employ biocatalysts, called enzymes, which are highly specific and catalytic towards the oxidation of a biofuel and the reduction of oxygen or hydrogen peroxide. Enzymes, on one hand, are promising candidates to replace expensive noble metal-based catalysts in fuel cell research. On the other hand, they offer the exciting prospect of a new generation of fuel cells which harvest energy from body fluids. Biofuel cells which use glucose as a fuel are particularly interesting for generating electricity to power electronic devices inside a living body. Hydrogen consuming biofuel cells represent an emerging alternative to platinum catalysts due to comparable efficiencies and the capability to operate at lower temperatures. Currently, these technologies are not competitive with existing commercialised fuel cell devices due to limitations including insufficient power outputs and lifetimes. The advantages and challenges facing glucose biofuel cells for implantation and hydrogen biofuel cells will be summarised along with recent promising advances and the future prospects of these exotic energy-harvesting devices.

  1. Fostering citizen deliberations on the social acceptability of renewable fuels policy: The case of advanced lignocellulosic biofuels in Canada

    International Nuclear Information System (INIS)

    It is widely recognized that a lack of social acceptance is likely to hinder the ability of governments to achieve policy targets concerning renewable energies. In this paper, we discuss the results of a pre- and post-test online survey that was conducted as part of the 2012 “Advanced Biofuels” deliberative democracy public engagement event in Montréal, Québec. The event sough to foster public learning and discussion in order to produce socially acceptable policy input for one type of renewable energy: advanced lignocellulosic biofuels. Survey results show that the majority of participants were strongly supportive of advanced lignocellulosic biofuel development in Canada after the deliberative event. By the end of the event, support also grew for current Canadian biofuel policies and many agreed that increasing biofuel production should be widely supported by the Canadian public. However, despite this support, about two thirds of participants revealed that they did not feel included in government decisions about biofuels. The gap between support after inclusive deliberation and expressed exclusion from Canadian government decisions points to the importance of fostering future citizen engagements in this area of renewable energy policy. - Highlights: • We analyze outputs from the 2012 “Advanced Biofuels” deliberative democracy event. • We focus on social acceptance levels of advanced lignocellulosic biofuels in Canada. • Participants became less supportive of using food crops after the deliberation. • The majority were also supportive of current federal policy after the event. • However, most did not feel included in government decisions about biofuels

  2. Water quality under increased biofuel production and future climate change and uncertainty

    Science.gov (United States)

    Demissie, Y. K.; Yan, E.

    2015-12-01

    Over the past decade, biofuel has emerged as an important renewable energy source to supplement gasoline and reduce the associated greenhouse gas emission. Many countries, for instant, have adopted biofuel production goals to blend 10% or more of gasoline with biofuels within 10 to 20 years. However, meeting these goals requires sustainable production of biofuel feedstock which can be challenging under future change in climate and extreme weather conditions, as well as the likely impacts of biofuel feedstock production on water quality and availability. To understand this interrelationship and the combined effects of increased biofuel production and climate change on regional and local water resources, we have performed watershed hydrology and water quality analyses for the Ohio River Basin. The basin is one of the major biofuel feedstock producing region in the United States, which also currently contributes about half of the flow and one third of phosphorus and nitrogen loadings to the Mississippi River that eventually flows to the Gulf of Mexico. The analyses integrate future scenarios and climate change and biofuel development through various mixes of landuse and agricultural management changes and examine their potential impacts on regional and local hydrology, water quality, soil erosion, and agriculture productivity. The results of the study are expected to provide much needed insight about the sustainability of large-scale biofuel feedstock production under the future climate change and uncertainty, and helps to further optimize the feedstock production taking into consideration the water-use efficiency.

  3. Microalgae as Sustainable Renewable Energy Feedstock for Biofuel Production

    OpenAIRE

    Srikanth Reddy Medipally; Fatimah Md. Yusoff; Sanjoy Banerjee; Shariff, M

    2015-01-01

    The world energy crisis and increased greenhouse gas emissions have driven the search for alternative and environmentally friendly renewable energy sources. According to life cycle analysis, microalgae biofuel is identified as one of the major renewable energy sources for sustainable development, with potential to replace the fossil-based fuels. Microalgae biofuel was devoid of the major drawbacks associated with oil crops and lignocelluloses-based biofuels. Algae-based biofuels are technical...

  4. Fields of dreams: Agriculture, economy and nature in Midwest United States biofuel production

    Science.gov (United States)

    Gillon, Sean Thomas

    This work explores the social and ecological dimensions of recent biofuel production increases in the United States (US), focusing on the case of Iowa. Biofuels are proposed to mitigate the greenhouse gas emissions that cause climate change, improve US energy security, and support rural economies. Little research has examined how increased US Midwestern biofuels production will change social and ecological outcomes at farm and regional levels or interact with broader governance processes at the nexus of agriculture, energy and environment. These broad questions guide my research: (1) How does biofuel production reconfigure agricultural practice and landscapes in Iowa? (2) What are the costs, benefits and risks of increased biofuels production as seen by farmers and rural residents, and how do these factors influence farmer decisions about agriculture and conservation practice? (3) How and with what effects are biofuels initiatives constituted as a form of environmental governance through scientific knowledge and practice and political economic dynamics? To address these questions, this research integrates both qualitative and quantitative methods, drawing on a political ecological approach complemented by agroecological analysis and theoretical insights from geographical analyses of nature-society relations. Quantitative analysis focuses on changing land use patterns in agriculture and conservation practice in Iowa. Qualitative methods include extensive interviews, participant observation, and policy and document analyses. Fieldwork focused on Northeastern Iowa to understand regional changes in agricultural and conservation practice, the renegotiated position of farmers in agriculture and biofuel production, and biofuel industry development. I find that biofuel production presents significant social and ecological challenges for rural places of production. Longstanding, unequal political economic relations in industrialized agriculture limit rural economic benefits

  5. Biofuel Production Initiative at Claflin University Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Chowdhury, Kamal

    2011-07-20

    For US transportation fuel independence or reduced dependence on foreign oil, the Federal Government has mandated that the country produce 36 billion gallons (bg) of renewable transportation fuel per year for its transportation fuel supply by 2022. This can be achieved only if development of efficient technology for second generation biofuel from ligno-cellulosic sources is feasible. To be successful in this area, development of a widely available, renewable, cost-effective ligno-cellulosic biomass feedstock that can be easily and efficiently converted biochemically by bacteria or other fast-growing organisms is required. Moreover, if the biofuel type is butanol, then the existing infrastructure to deliver fuel to the customer can be used without additional costs and retrofits. The Claflin Biofuel Initiative project is focused on helping the US meet the above-mentioned targets. With support from this grant, Claflin University (CU) scientists have created over 50 new strains of microorganisms that are producing butanol from complex carbohydrates and cellulosic compounds. Laboratory analysis shows that a number of these strains are producing higher percentages of butanol than other methods currently in use. All of these recombinant bacterial strains are producing relatively high concentrations of acetone and numerous other byproducts as well. Therefore, we are carrying out intense mutations in the selected strains to reduce undesirable byproducts and increase the desired butanol production to further maximize the yield of butanol. We are testing the proof of concept of producing pre-industrial large scale biobutanol production by utilizing modifications of currently commercially available fermentation technology and instrumentation. We have already developed an initial process flow diagram (PFD) and selected a site for a biobutanol pilot scale facility in Orangeburg, SC. With the recent success in engineering new strains of various biofuel producing bacteria at CU

  6. 75 FR 50986 - Notice of Contract Proposal (NOCP) for Payments to Eligible Advanced Biofuel Producers

    Science.gov (United States)

    2010-08-18

    ... 12, 2010 at 75 FR 11836 for the distribution of the remaining available Fiscal Year 2009 program...) requested advanced biofuels producers determined eligible under the June 12, 2009, NOCP (74 FR 27998) to... determined eligible under the June 12, 2009 NOCP (74 FR 27998) to submit a request for additional payment...

  7. Biofuel Database

    Science.gov (United States)

    Biofuel Database (Web, free access)   This database brings together structural, biological, and thermodynamic data for enzymes that are either in current use or are being considered for use in the production of biofuels.

  8. Overcoming the initial investment hurdle for advanced biofuels. An analysis of biofuel-related risks and their impact on project financing. Report of ELOBIO subtask 7

    International Nuclear Information System (INIS)

    The ELOBIO research project aims to develop policies that will help achieve a higher share of biofuels in total transport fuel in a low-disturbing and sustainable way. Workpackage 7 of the ELOBIO project aims at addressing the objective of providing a reliable estimate of the potential and costs of biofuels, given the application of low-disturbing policy measures. More specifically, we seek to evaluate the impact of these biofuel policy measures on the investment climate for second-generation technologies. To this end, we try to answer several sub-questions in a following logical sequence: (1) What are the different factors that contribute to investment risk in biofuels and what are their relative contributions to overall biofuel project risk as perceived by finance providers?; (2) How do these risks translate into cost of capital for different biofuel technologies?; (3) How does cost of capital influence market penetration rates for the different technologies?; and (4) What is the best policy (or policy mix) to overcome the initial investment hurdle for advanced biofuels, thus lowering their cost of capital and achieve wider market deployment?.

  9. Burkholderia cepacia lipase is a promising biocatalyst for biofuel production.

    Science.gov (United States)

    Sasso, Francesco; Natalello, Antonino; Castoldi, Simone; Lotti, Marina; Santambrogio, Carlo; Grandori, Rita

    2016-07-01

    Lipases resistant to inhibition and denaturation by methanol are valuable tools for biotechnological applications, in particular for biofuel production. Microbial lipases have attracted a great deal of interest because of their stability at high concentrations of organic solvents. Burkholderia cepacia lipase (BCL) is tested here for robustness towards methanol in terms of conformational stability and catalytic activity in transesterification assays. This lipase turns out to be even more tolerant than the homologous and better characterized enzyme from Burkholderia glumae. BCL unfolding transition, as monitored by far-UV circular dichroism (CD) and intrinsic fluorescence, displays a Tm above 60°C in the presence of 50% methanol. The protein unfolds at low pH, and the organic solvent affects the nature of the denatured state under acidic conditions. The protein performs well in transesterification assays upon prolonged incubations at high methanol concentrations. BCL is highly tolerant to methanol and displays particularly high conformational stability under conditions employed for transesterification reactions. These features depict BCL as a promising enzyme for biofuel industry. PMID:27067648

  10. Biofuel Production Using Butanol and Used Transformer Oil

    Directory of Open Access Journals (Sweden)

    J.Antony Pradeep

    2016-08-01

    Full Text Available Energy demand of the world increases tremendously. The use of fossil fuels to this extent makes it limited and it is an important cause for global warming. To reduce the emission of greenhouse gases and fossil fuel usage, many developments were made using renewable energy. Ethanol was used as a substitute for gasoline lately but it is highly corrosive and need separate tankers for transportation. Instead of using ethanol, butanol will give high mileage, less corrosive and has higher flash and boiling point than ethanol. In this project, biofuel is produced by transesterification of butanol and used transformer oil with KOH as catalyst. Glycerol is a by-product in this process. It can be used as a substrate for the microbial production of plastics and in anaerobic digestion.

  11. 75 FR 42745 - Production Incentives for Cellulosic Biofuels: Notice of Program Intent

    Science.gov (United States)

    2010-07-22

    ..., ``Production Incentives for Cellulosic Biofuels; Reverse Auction Procedures and Standards,'' (74 FR 52867... month period; and (v) Identify one or more proposed sources of financing for the construction or expansion of the filing party's eligible cellulosic biofuels production facility. DOE will provide...

  12. The economic feasibility of sugar beet biofuel production in central North Dakota

    International Nuclear Information System (INIS)

    This study examines the financial feasibility of producing ethanol biofuel from sugar beets in central North Dakota. Under the Energy Independence and Security Act (EISA) of 2007, biofuel from sugar beets uniquely qualifies as an 'advanced biofuel'. EISA mandates production of 21 billion gallons of advanced biofuels annually by 2022. A stochastic simulation financial model was calibrated with irrigated sugar beet data from central North Dakota to determine economic feasibility and risks of production for 0.038 hm3y-1 (or 10 MGY (Million Gallon per Year) and 0.076 hm3y-1 (or 20 MGY) ethanol plants. Study results indicate that feedstock costs, which include sugar beets and beet molasses, account for more than 70 percent of total production expenses. The estimated breakeven ethanol price for the 0.076 hm3y-1 plant is $400 m-3 ($1.52 per gallon) and $450 m-3 ($1.71 per gallon) for the 0.038 hm3y-1 plant. Breakeven prices for feedstocks are also estimated and show that the 0.076 hm3y-1 plant can tolerate greater ethanol and feedstock price risks than the 0.038 hm3y-1 plant. Our results also show that one of the most important factors that affect investment success is the price of ethanol. At an ethanol price of $484.21 m-3 ($1.84 per gallon), and assuming other factors remain unchanged, the estimated net present value (NPV) for the 0.076 hm3y-1 plant is $41.54 million. By comparison, the estimated NPV for the 0.038 hm3y-1 plant is only $8.30 million. Other factors such as changes in prices of co-products and utilities have a relatively minor effect on investment viability. -- Highlights: → Sugar beets and beet molasses costs account for more than 70 percent of total production expenses. → The estimated breakeven ethanol prices for the 0.076 hm3y-1 and 0.038 hm3y-1ethanol plants are $400 m-3 and $450 m-3 respectively. → The price of ethanol will be one of the most important factors for determining the future feasibility of a sugar-beet-based ethanol plant in North

  13. Synthetic biology for microbial production of lipid-based biofuels.

    Science.gov (United States)

    d'Espaux, Leo; Mendez-Perez, Daniel; Li, Rachel; Keasling, Jay D

    2015-12-01

    The risks of maintaining current CO2 emission trends have led to interest in producing biofuels using engineered microbes. Microbial biofuels reduce emissions because CO2 produced by fuel combustion is offset by CO2 captured by growing biomass, which is later used as feedstock for biofuel fermentation. Hydrocarbons found in petroleum fuels share striking similarity with biological lipids. Here we review synthetic metabolic pathways based on fatty acid and isoprenoid metabolism to produce alkanes and other molecules suitable as biofuels. We further discuss engineering strategies to optimize engineered biosynthetic routes, as well as the potential of synthetic biology for sustainable manufacturing.

  14. Synthetic biology for microbial production of lipid-based biofuels.

    Science.gov (United States)

    d'Espaux, Leo; Mendez-Perez, Daniel; Li, Rachel; Keasling, Jay D

    2015-12-01

    The risks of maintaining current CO2 emission trends have led to interest in producing biofuels using engineered microbes. Microbial biofuels reduce emissions because CO2 produced by fuel combustion is offset by CO2 captured by growing biomass, which is later used as feedstock for biofuel fermentation. Hydrocarbons found in petroleum fuels share striking similarity with biological lipids. Here we review synthetic metabolic pathways based on fatty acid and isoprenoid metabolism to produce alkanes and other molecules suitable as biofuels. We further discuss engineering strategies to optimize engineered biosynthetic routes, as well as the potential of synthetic biology for sustainable manufacturing. PMID:26479184

  15. System studies on Biofuel production via Integrated Biomass Gasification

    Energy Technology Data Exchange (ETDEWEB)

    Andersson, Jim; Lundgren, Joakim [Luleaa Univ. of Technology Bio4Energy, Luleaa (Sweden); Malek, Laura; Hulteberg, Christian [Lund Univ., Lund (Sweden); Pettersson, Karin [Chalmers Univ. of Technology, Goeteborg (Sweden); Wetterlund, Elisabeth [Linkoeping Univ. Linkoeping (Sweden)

    2013-09-01

    A large number of national and international techno-economic studies on industrially integrated gasifiers for production of biofuels have been published during the recent years. These studies comprise different types of gasifiers (fluidized bed, indirect and entrained flow) integrated in different industries for the production of various types of chemicals and transportation fuels (SNG, FT-products, methanol, DME etc.) The results are often used for techno-economic comparisons between different biorefinery concepts. One relatively common observation is that even if the applied technology and the produced biofuel are the same, the results of the techno-economic studies may differ significantly. The main objective of this project has been to perform a comprehensive review of publications regarding industrially integrated biomass gasifiers for motor fuel production. The purposes have been to identify and highlight the main reasons why similar studies differ considerably and to prepare a basis for fair techno-economic comparisons. Another objective has been to identify possible lack of industrial integration studies that may be of interest to carry out in a second phase of the project. Around 40 national and international reports and articles have been analysed and reviewed. The majority of the studies concern gasifiers installed in chemical pulp and paper mills where black liquor gasification is the dominating technology. District heating systems are also well represented. Only a few studies have been found with mechanical pulp and paper mills, steel industries and the oil refineries as case basis. Other industries have rarely, or not at all, been considered for industrial integration studies. Surprisingly, no studies regarding integration of biomass gasification neither in saw mills nor in wood pellet production industry have been found. In the published economic evaluations, it has been found that there is a large number of studies containing both integration and

  16. Economic and social implications of biofuel use and production in Canada

    International Nuclear Information System (INIS)

    The potential role of biofuels in meeting Canadian commitments to greenhouse gas emissions was discussed. The characteristics of various biofuels were presented, including ethanol, methanol, biodiesel and biogas. Benefits of biofuels included a reduction in air contaminants as well as lower greenhouse gas emissions. Federal and provincial programs are currently in place to encourage production and use of biofuels. The Federal Ethanol Expansion Plan was outlined with reference to its target to increase ethanol production from 238 m litres to 1400 m litres by 2010. The main instruments of the program include excision of the gasoline tax exemption, ethanol expansion and the fact that ethanol can operate a polyfuels vehicle fleet. Provincial policies on ethanol were outlined, driven by characteristics of provincial economies. Provincial tax exemptions for ethanol were provided and an overview of the global ethanol market was presented. A map of existing and projected ethanol projects in Canada was presented, along with a forecast of Canadian ethanol production capacity. A time-line of Nebraska's ethanol production from the years 1985 to 2004 was provided. Economic drivers for ethanol include additional markets for products of agricultural, marine and forestry industries; the enhancement and diversification of rural and regional economies; employment; and energy security. Challenges to growth in biofuel production include technological knowledge and a lack of public awareness concerning the benefits of biofuel. The production and use of biofuels may increase environmental amenities but decrease economic growth. Issues concerning the economics of biofuel research were reviewed. The demand for biofuels has grown slowly in Canada, but has been promoted or mandated federally and in several provinces. The costs of biofuel production were reviewed, with a chart presenting ethanol production costs by plant size. Barriers to trade include the complexity of provincial tax

  17. A model for sustainable land use in biofuel production: An application to the state of Alabama

    International Nuclear Information System (INIS)

    The Renewable Fuel Standard aims to increase the production of biofuels to improve energy efficiency and decrease carbon dioxide emissions in the US. The effectiveness of this regulation is being debated by the scientific community regarding carbon emissions from direct and indirect land-use change. A valid alternative may be to design policies that stimulate sustainable land use in biofuel production. This article develops a model that simulates a voluntary program to increase the land use efficiency in production of biofuels. This stochastic dynamic model optimizes the sustainability of biofuels producible by including climate information and participatory decisions on land use. The model is parameterized using the Maximum Entropy econometric technique to present a simulation of the program in the State of Alabama. The results of this simulation show that participatory decisions on land-use may increase the net energy value of produced biofuel up to 215.68% and reduce the carbon emissions by 19.67% towards the state energy goals. - Highlights: ► We develop a dynamic model that optimizes the land-use efficiency of biofuels. ► This model simulates the impact of a voluntary program on sustainability of biofuels. ► Entropy econometrics are used to calibrate the model for the state of Alabama. ► Participatory decisions on land-use may increase producible bioenergy in the state. ► A sustainability program may reduce carbon emissions in biofuel production

  18. Cyanobacterial chassis engineering for enhancing production of biofuels and chemicals.

    Science.gov (United States)

    Gao, Xinyan; Sun, Tao; Pei, Guangsheng; Chen, Lei; Zhang, Weiwen

    2016-04-01

    To reduce dependence on fossil fuels and curb greenhouse effect, cyanobacteria have emerged as an important chassis candidate for producing biofuels and chemicals due to their capability to directly utilize sunlight and CO2 as the sole energy and carbon sources, respectively. Recent progresses in developing and applying various synthetic biology tools have led to the successful constructions of novel pathways of several dozen green fuels and chemicals utilizing cyanobacterial chassis. Meanwhile, it is increasingly recognized that in order to enhance productivity of the synthetic cyanobacterial systems, optimizing and engineering more robust and high-efficient cyanobacterial chassis should not be omitted. In recent years, numerous research studies have been conducted to enhance production of green fuels and chemicals through cyanobacterial chassis modifications involving photosynthesis, CO2 uptake and fixation, products exporting, tolerance, and cellular regulation. In this article, we critically reviewed recent progresses and universal strategies in cyanobacterial chassis engineering to make it more robust and effective for bio-chemicals production. PMID:26883347

  19. Use of tamarisk as a potential feedstock for biofuel production.

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Amy Cha-Tien; Norman, Kirsten

    2011-01-01

    This study assesses the energy and water use of saltcedar (or tamarisk) as biomass for biofuel production in a hypothetical sub-region in New Mexico. The baseline scenario consists of a rural stretch of the Middle Rio Grande River with 25% coverage of mature saltcedar that is removed and converted to biofuels. A manufacturing system life cycle consisting of harvesting, transportation, pyrolysis, and purification is constructed for calculating energy and water balances. On a dry short ton woody biomass basis, the total energy input is approximately 8.21 mmBTU/st. There is potential for 18.82 mmBTU/st of energy output from the baseline system. Of the extractable energy, approximately 61.1% consists of bio-oil, 20.3% bio-char, and 18.6% biogas. Water consumptive use by removal of tamarisk will not impact the existing rate of evapotranspiration. However, approximately 195 gal of water is needed per short ton of woody biomass for the conversion of biomass to biocrude, three-quarters of which is cooling water that can be recovered and recycled. The impact of salt presence is briefly assessed. Not accounted for in the baseline are high concentrations of Calcium, Sodium, and Sulfur ions in saltcedar woody biomass that can potentially shift the relative quantities of bio-char and bio-oil. This can be alleviated by a pre-wash step prior to the conversion step. More study is needed to account for the impact of salt presence on the overall energy and water balance.

  20. Gaining ground in the modeling of land-use change greenhouse gas emissions associated with biofuel production

    Science.gov (United States)

    Dunn, J.; Mueller, S.; Kwon, H.; Wang, M.; Wander, M.

    2012-12-01

    Land-use change (LUC) resulting from biofuel feedstock production and the associated greenhouse gas (GHG) emissions are a hotly-debated aspect of biofuels. Certainly, LUC GHG emissions are one of the most uncertain elements in life cycle analyses (LCA) of biofuels. To estimate LUC GHG emissions, two sets of data are necessary. First, information on the amount and type of land that is converted to biofuel feedstock production is required. These data are typically generated through application of computable general equilibrium (CGE) models such as Purdue University's Global Trade Analysis Project (GTAP) model. Second, soil carbon content data for the affected land types is essential. Recently, Argonne National Laboratory's Carbon Calculator for Land Use Change from Biofuels Production (CCLUB) has been updated with CGE modeling results that estimate the amount and type of LUC world-wide from production of ethanol from corn, corn stover, miscanthus, and switchgrass (Mueller et al. 2012). Moreover, we have developed state-specific carbon content data, determined through modeling with CENTURY, for the two most dominant soil types in the conterminous 48 U.S. states (Kwon et al. 2012) to enable finer-resolution results for domestic LUC GHG emissions for these ethanol production scenarios. Of the feedstocks examined, CCLUB estimates that LUC GHG emissions are highest for corn ethanol (9.1 g CO2e/MJ ethanol) and lowest for miscanthus (-12 g CO2e/MJ ethanol). We will present key observations from CCLUB results incorporated into Argonne National Laboratory's Greenhouse Gases, Regulated Emissions, and Energy use in Transportation (GREET) model, which is a LCA tool for transportation fuels and advanced vehicle technologies. We will discuss selected issues in this modeling, including the sensitivity of domestic soil carbon emission factors to modeling parameters and assumptions about the fate of harvested wood products. Further, we will discuss efforts to update CCLUB with county

  1. Indirect Land Use Change From Increased Biofuels Demand - Comparison of Models and Results for Marginal Biofuels Production from Different Feedstocks

    OpenAIRE

    Edwards, Robert; MULLIGAN DECLAN; Marelli, Luisa

    2010-01-01

    This study compares the ILUC results produced by different economic models for marginal increases in biofuel production from different feedstocks. The work is the result of a survey of marginal calculations launched by the JRC-IE during 2009, involving some of the best known models worldwid. The modellers were requested by JRC-IE to run scenarios corresponding as closely as possible to the following specification (e.g. marginal runs against existing baseline of the following scenarios): A ...

  2. "Trojan Horse" strategy for deconstruction of biomass for biofuels production.

    Energy Technology Data Exchange (ETDEWEB)

    Sinclair, Michael B.; Hadi, Masood Z.; Timlin, Jerilyn Ann; Thomson, James (USDA, Albany, CA); Whalen, Maureen (USDA, Albany, CA); Thilmony, Roger (USDA, Albany, CA); Tran-Gyamfi, Mary; Simmons, Blake Alexander; Sapra, Rajat

    2008-08-01

    Production of renewable biofuels to displace fossil fuels currently consumed in the transportation sector is a pressing multi-agency national priority. Currently, nearly all fuel ethanol is produced from corn-derived starch. Dedicated 'energy crops' and agricultural waste are preferred long-term solutions for renewable, cheap, and globally available biofuels as they avoid some of the market pressures and secondary greenhouse gas emission challenges currently facing corn ethanol. These sources of lignocellulosic biomass are converted to fermentable sugars using a variety of chemical and thermochemical pretreatments, which disrupt cellulose and lignin cross-links, allowing exogenously added recombinant microbial enzymes to more efficiently hydrolyze the cellulose for 'deconstruction' into glucose. This process is plagued with inefficiencies, primarily due to the recalcitrance of cellulosic biomass, mass transfer issues during deconstruction, and low activity of recombinant deconstruction enzymes. Costs are also high due to the requirement for enzymes and reagents, and energy-intensive and cumbersome pretreatment steps. One potential solution to these problems is found in synthetic biology; they propose to engineer plants that self-produce a suite of cellulase enzymes targeted to the apoplast for cleaving the linkages between lignin and cellulosic fibers; the genes encoding the degradation enzymes, also known as cellulases, are obtained from extremophilic organisms that grow at high temperatures (60-100 C) and acidic pH levels (<5). These enzymes will remain inactive during the life cycle of the plant but become active during hydrothermal pretreatment i.e., elevated temperatures. Deconstruction can be integrated into a one-step process, thereby increasing efficiency (cellulose-cellulase mass-transfer rates) and reducing costs. The proposed disruptive technologies address biomass deconstruction processes by developing transgenic plants encoding a

  3. Final Technical Report - Use of Systems Biology Approaches to Develop Advanced Biofuel-Synthesizing Cyanobacterial Strains

    Energy Technology Data Exchange (ETDEWEB)

    Pakrasi, Himadri [Washington Univ., St. Louis, MO (United States)

    2016-09-01

    The overall objective of this project was to use a systems biology approach to evaluate the potentials of a number of cyanobacterial strains for photobiological production of advanced biofuels and/or their chemical precursors. Cyanobacteria are oxygen evolving photosynthetic prokaryotes. Among them, certain unicellular species such as Cyanothece can also fix N2, a process that is exquisitely sensitive to oxygen. To accommodate such incompatible processes in a single cell, Cyanothece produces oxygen during the day, and creates an O2-limited intracellular environment during the night to perform O2-sensitive processes such as N2-fixation. Thus, Cyanothece cells are natural bioreactors for the storage of captured solar energy with subsequent utilization at a different time during a diurnal cycle. Our studies include the identification of a novel, fast-growing, mixotrophic, transformable cyanobacterium. This strain has been sequenced and will be made available to the community. In addition, we have developed genome-scale models for a family of cyanobacteria to assess their metabolic repertoire. Furthermore, we developed a method for rapid construction of metabolic models using multiple annotation sources and a metabolic model of a related organism. This method will allow rapid annotation and screening of potential phenotypes based on the newly available genome sequences of many organisms.

  4. Dynamic Modeling of Learning in Emerging Energy Industries: The Example of Advanced Biofuels in the United States; NREL (National Renewable Energy Laboratory)

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, Steve; Bush, Brian; Vimmerstedt, Laura

    2015-07-19

    This paper (and its supplemental model) presents novel approaches to modeling interactions and related policies among investment, production, and learning in an emerging competitive industry. New biomass-to-biofuels pathways are being developed and commercialized to support goals for U.S. advanced biofuel use, such as those in the Energy Independence and Security Act of 2007. We explore the impact of learning rates and techno-economics in a learning model excerpted from the Biomass Scenario Model (BSM), developed by the U.S. Department of Energy and the National Renewable Energy Laboratory to explore the impact of biofuel policy on the evolution of the biofuels industry. The BSM integrates investment, production, and learning among competing biofuel conversion options that are at different stages of industrial development. We explain the novel methods used to simulate the impact of differing assumptions about mature industry techno-economics and about learning rates while accounting for the different maturity levels of various conversion pathways. A sensitivity study shows that the parameters studied (fixed capital investment, process yield, progress ratios, and pre-commercial investment) exhibit highly interactive effects, and the system, as modeled, tends toward market dominance of a single pathway due to competition and learning dynamics.

  5. From Pandemic Preparedness to Biofuel Production: Tobacco Finds Its Biotechnology Niche in North America

    Directory of Open Access Journals (Sweden)

    Joshua D. Powell

    2015-09-01

    Full Text Available In 2012 scientists funded by the United States Defense Advanced Research Projects Agency (DARPA produced 10 million doses of influenza vaccine in tobacco in a milestone deadline of one month. Recently the experimental antibody cocktail Zmapp™, also produced in tobacco, has shown promise as an emergency intervention therapeutic against Ebola virus. These two examples showcase how collaborative efforts between government, private industry and academia are applying plant biotechnology to combat pathogenic agents. Opportunities now exist repurposing tobacco expression systems for exciting new applications in synthetic biology, biofuels production and industrial enzyme production. As plant-produced biotherapeutics become more mainstream, government funding agencies need to be cognizant of the idea that many plant-produced biologicals are often safer, cheaper, and just as efficacious as traditionally used expression systems.

  6. Integrated Algae Cultivation for Biofuels Production in Industrial Clusters

    OpenAIRE

    Broberg, Sarah; Andersson, Vikor; Hackl, Roman

    2011-01-01

    Declining fossil resources and the issue of climate change caused by anthropogenic emissions of greenhouse gases make global action towards a more sustainable society inevitable. The EU decided in 2007 that 20 % of the union´s energy use should origin from renewable resources by the year 2020. One way of achieving this goal is to increase the utilisation of biofuels. Today 2nd generation biofuels are being developed. They are seen as a more sustainable solution than 1st generation biofuels si...

  7. Biofuel supply chain, market, and policy analysis

    Science.gov (United States)

    Zhang, Leilei

    Renewable fuel is receiving an increasing attention as a substitute for fossil based energy. The US Department of Energy (DOE) has employed increasing effort on promoting the advanced biofuel productions. Although the advanced biofuel remains at its early stage, it is expected to play an important role in climate policy in the future in the transportation sector. This dissertation studies the emerging biofuel supply chain and markets by analyzing the production cost, and the outcomes of the biofuel market, including blended fuel market price and quantity, biofuel contract price and quantity, profitability of each stakeholder (farmers, biofuel producers, biofuel blenders) in the market. I also address government policy impacts on the emerging biofuel market. The dissertation is composed with three parts, each in a paper format. The first part studies the supply chain of emerging biofuel industry. Two optimization-based models are built to determine the number of facilities to deploy, facility locations, facility capacities, and operational planning within facilities. Cost analyses have been conducted under a variety of biofuel demand scenarios. It is my intention that this model will shed light on biofuel supply chain design considering operational planning under uncertain demand situations. The second part of the dissertation work focuses on analyzing the interaction between the key stakeholders along the supply chain. A bottom-up equilibrium model is built for the emerging biofuel market to study the competition in the advanced biofuel market, explicitly formulating the interactions between farmers, biofuel producers, blenders, and consumers. The model simulates the profit maximization of multiple market entities by incorporating their competitive decisions in farmers' land allocation, biomass transportation, biofuel production, and biofuel blending. As such, the equilibrium model is capable of and appropriate for policy analysis, especially for those policies

  8. An Overview of Algae Biofuel Production and Potential Environmental Impact

    Science.gov (United States)

    Algae are among the most potentially significant sources of sustainable biofuels in the future of renewable energy. A feedstock with virtually unlimited applicability, algae can metabolize various waste streams (e.g., municipal wastewater, carbon dioxide from industrial flue gas)...

  9. Innovation in biological production and upgrading of methane and hydrogen for use as gaseous transport biofuel.

    Science.gov (United States)

    Xia, Ao; Cheng, Jun; Murphy, Jerry D

    2016-01-01

    Biofuels derived from biomass will play a major role in future renewable energy supplies in transport. Gaseous biofuels have superior energy balances, offer greater greenhouse gas emission reductions and produce lower pollutant emissions than liquid biofuels. Biogas derived through fermentation of wet organic substrates will play a major role in future transport systems. Biogas (which is composed of approximately 60% methane/hydrogen and 40% carbon dioxide) requires an upgrading process to reduce the carbon dioxide content to less than 3% before it is used as compressed gas in transport. This paper reviews recent developments in fermentative biogas production and upgrading as a transport fuel. Third generation gaseous biofuels may be generated using marine-based algae via two-stage fermentation, cogenerating hydrogen and methane. Alternative biological upgrading techniques, such as biological methanation and microalgal biogas upgrading, have the potential to simultaneously upgrade biogas, increase gaseous biofuel yield and reduce carbon dioxide emission. PMID:26724182

  10. Biofuel production potentials in Europe: Sustainable use of cultivated land and pastures. Part I: Land productivity potentials

    NARCIS (Netherlands)

    Fischer, G.; Prieler, S.; van Velthuizen, H.; Lensink, S.; Londo, H.M.; de Wit, M.P.

    2009-01-01

    IIASA's agro-ecological zones modelling framework has been extended for biofuel productivity assessments distinguishing five main groups of feedstocks covering a wide range of agronomic conditions and energy production pathways, namely: woody lignocellulosic plants, herbaceous lignocellulosic plants

  11. Comprehensive techno-economic analysis of wastewater-based algal biofuel production: A case study.

    Science.gov (United States)

    Xin, Chunhua; Addy, Min M; Zhao, Jinyu; Cheng, Yanling; Cheng, Sibo; Mu, Dongyan; Liu, Yuhuan; Ding, Rijia; Chen, Paul; Ruan, Roger

    2016-07-01

    Combining algae cultivation and wastewater treatment for biofuel production is considered the feasible way for resource utilization. An updated comprehensive techno-economic analysis method that integrates resources availability into techno-economic analysis was employed to evaluate the wastewater-based algal biofuel production with the consideration of wastewater treatment improvement, greenhouse gases emissions, biofuel production costs, and coproduct utilization. An innovative approach consisting of microalgae cultivation on centrate wastewater, microalgae harvest through flocculation, solar drying of biomass, pyrolysis of biomass to bio-oil, and utilization of co-products, was analyzed and shown to yield profound positive results in comparison with others. The estimated break even selling price of biofuel ($2.23/gallon) is very close to the acceptable level. The approach would have better overall benefits and the internal rate of return would increase up to 18.7% if three critical components, namely cultivation, harvest, and downstream conversion could achieve breakthroughs. PMID:27039331

  12. Sustainable Production of Second-Generation Biofuels. Potential and perspectives in major economies and developing countries

    International Nuclear Information System (INIS)

    The paper focuses on opportunities and risks presented by second-generation biofuels technologies in eight case study countries: Brazil, Cameroon, China, India, Mexico, South Africa, Tanzania and Thailand. The report begins by exploring the state of the art of second-generation technologies and their production, followed by projections of future demand and a discussion of drivers of that demand. The report then delves into various feedstock options and the global potential for bioenergy production. The final chapter offers a look at the potential for sustainable second-generation biofuel production in developing countries including considerations of economic, social and environmental impacts. Key findings of the report include that: second-generation biofuels produced from agricultural and forestry residues can play a crucial role in the transport sector without competing with food production; the potential for second-generation biofuels should be mobilized in emerging and developing countries where a large share of global residues is produced; less-developed countries will first need to invest in agricultural production and infrastructure in order to improve the framework conditions for the production of second-generation biofuels; financial barriers to production exist in many developing countries; and the suitability of second-generation biofuels against individual developing countries' needs should be evaluated.

  13. Sustainable Production of Second-Generation Biofuels. Potential and perspectives in major economies and developing countries

    Energy Technology Data Exchange (ETDEWEB)

    Eisentraut, A.

    2010-02-15

    The paper focuses on opportunities and risks presented by second-generation biofuels technologies in eight case study countries: Brazil, Cameroon, China, India, Mexico, South Africa, Tanzania and Thailand. The report begins by exploring the state of the art of second-generation technologies and their production, followed by projections of future demand and a discussion of drivers of that demand. The report then delves into various feedstock options and the global potential for bioenergy production. The final chapter offers a look at the potential for sustainable second-generation biofuel production in developing countries including considerations of economic, social and environmental impacts. Key findings of the report include that: second-generation biofuels produced from agricultural and forestry residues can play a crucial role in the transport sector without competing with food production; the potential for second-generation biofuels should be mobilized in emerging and developing countries where a large share of global residues is produced; less-developed countries will first need to invest in agricultural production and infrastructure in order to improve the framework conditions for the production of second-generation biofuels; financial barriers to production exist in many developing countries; and the suitability of second-generation biofuels against individual developing countries' needs should be evaluated.

  14. Performance assessment of biofuel production via biomass fast pyrolysis and refinery technologies

    OpenAIRE

    Shemfe, Mobolaji B.

    2016-01-01

    Biofuels have been identified as one of several GHG emission strategies to reduce the use of fossil fuels in the transport sector. Fast pyrolysis of biomass is one approach to producing second generation biofuels. The bio-oil product of fast pyrolysis can be upgraded into essential gasoline and diesel range products with conventional refinery technologies. Thus, it is important to assess their techno- economic and environmental performance at an early stage prior to commerci...

  15. The effect of feedstock cost on biofuel cost as exemplified by biomethane production from grass silage.

    OpenAIRE

    McEniry, J.; O’Kiely, P.; Crosson, P.; Groom, Elaine; Murphy, J. D.

    2011-01-01

    Abstract: The potential variance in feedstock costs can have signifi cant implications for the cost of a biofuel and the fi nancial viability of a biofuel facility. This paper employs the Grange Feed Costing Model to assess the cost of on-farm biomethane production using grass silages produced under a range of management scenarios. These costs were compared with the cost of wheat grain and sugarbeet roots for ethanol production at an industrial scale. Of the three feedstocks examined, grass s...

  16. Estimation of Economic Impacts of Cellulosic Biofuel Production: A Comparative Analysis of Three Biofuel Pathways

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yimin; Goldberg, Marshall; Tan, Eric; Meyer, Pimphan Aye

    2016-05-01

    The development of a cellulosic biofuel industry utilizing domestic biomass resources is expected to create opportunities for economic growth resulting from the construction and operation of new biorefineries. We applied an economic input-output model to estimate potential economic impacts, particularly gross job growth, resulting from the construction and operation of biorefineries using three different technology pathways: (i) cellulosic ethanol via biochemical conversion in Iowa, (ii) renewable diesel blendstock via biological conversion in Georgia, and (iii) renewable diesel and gasoline blendstock via fast pyrolysis in Mississippi. Combining direct, indirect (revenue- and supply-chain-related), and induced effects, capital investment associated with the construction of a biorefinery processing 2000 dry metric tons of biomass per day (DMT/day) could yield between 5960 and 8470 full-time equivalent (FTE) jobs during the construction period, depending on the biofuel pathways. Fast pyrolysis biorefineries produce the most jobs on a project level thanks to the highest capital requirement among the three pathways. Normalized on the scale of $1 million of capital investment, the fast pyrolysis biorefineries are estimated to yield slighter higher numbers of jobs (12.1 jobs) than the renewable diesel (11.8 jobs) and the cellulosic ethanol (11.6 jobs) biorefineries. While operating biorefineries is not labor-intensive, the annual operation of a 2000 DMT/day biorefinery could support between 720 and 970 jobs when the direct, indirect, and induced effects are considered. The major factor, which results in the variations among the three pathways, is the type of biomass feedstock used for biofuels. Unlike construction jobs, these operation-related jobs are necessary over the entire life of the biorefineries. Our results show that indirect effects stimulated by the operation of biorefineries are the primary contributor to job growth. The agriculture/forest, services, and

  17. Trash to treasure: production of biofuels and commodity chemicals via syngas fermenting microorganisms.

    Science.gov (United States)

    Latif, Haythem; Zeidan, Ahmad A; Nielsen, Alex T; Zengler, Karsten

    2014-06-01

    Fermentation of syngas is a means through which unutilized organic waste streams can be converted biologically into biofuels and commodity chemicals. Despite recent advances, several issues remain which limit implementation of industrial-scale syngas fermentation processes. At the cellular level, the energy conservation mechanism of syngas fermenting microorganisms has not yet been entirely elucidated. Furthermore, there was a lack of genetic tools to study and ultimately enhance their metabolic capabilities. Recently, substantial progress has been made in understanding the intricate energy conservation mechanisms of these microorganisms. Given the complex relationship between energy conservation and metabolism, strain design greatly benefits from systems-level approaches. Numerous genetic manipulation tools have also been developed, paving the way for the use of metabolic engineering and systems biology approaches. Rational strain designs can now be deployed resulting in desirable phenotypic traits for large-scale production. PMID:24863900

  18. A comprehensive review of biomass resources and biofuel production in Nigeria: potential and prospects.

    Science.gov (United States)

    Sokan-Adeaga, Adewale Allen; Ana, Godson R E E

    2015-01-01

    The quest for biofuels in Nigeria, no doubt, represents a legitimate ambition. This is so because the focus on biofuel production has assumed a global dimension, and the benefits that may accrue from such effort may turn out to be enormous if the preconditions are adequately satisfied. As a member of the global community, it has become exigent for Nigeria to explore other potential means of bettering her already impoverished economy. Biomass is the major energy source in Nigeria, contributing about 78% of Nigeria's primary energy supply. In this paper, a comprehensive review of the potential of biomass resources and biofuel production in Nigeria is given. The study adopted a desk review of existing literatures on major energy crops produced in Nigeria. A brief description of the current biofuel developmental activities in the country is also given. A variety of biomass resources exist in the country in large quantities with opportunities for expansion. Biomass resources considered include agricultural crops, agricultural crop residues, forestry resources, municipal solid waste, and animal waste. However, the prospects of achieving this giant stride appear not to be feasible in Nigeria. Although the focus on biofuel production may be a worthwhile endeavor in view of Nigeria's development woes, the paper argues that because Nigeria is yet to adequately satisfy the preconditions for such program, the effort may be designed to fail after all. To avoid this, the government must address key areas of concern such as food insecurity, environmental crisis, and blatant corruption in all quarters. It is concluded that given the large availability of biomass resources in Nigeria, there is immense potential for biofuel production from these biomass resources. With the very high potential for biofuel production, the governments as well as private investors are therefore encouraged to take practical steps toward investing in agriculture for the production of energy crops and the

  19. Catalyst technology for biofuel production: Conversion of renewable lipids into biojet and biodiesel

    Directory of Open Access Journals (Sweden)

    Scharff Yves

    2013-09-01

    Full Text Available Renewable lipids based biofuels are an important tool to address issues raised by policies put in place in order to reduce the dependence of transportation sector on fossil fuels and to promote the development of non-food based, sustainable and eco-friendly fuels. This paper presents the main features of the heterogeneous catalysis technologies Axens has developed for the production of biofuels from renewable lipids: the first by transesterification to produce fatty acid methyl esters or biodiesel and the second by hydrotreating to produce isoparaffinic hydroprocessed ester and fatty acids, high blending rate drop-in diesel and jet biofuels.

  20. Noble metal catalysts in the production of biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Gutierrez, A.

    2013-11-01

    The energy demand is increasing in the world together with the need to ensure energy security and the desire to decrease greenhouse gas emissions. While several renewable alternatives are available for the production of electricity, e.g. solar energy, wind power, and hydrogen, biomass is the only renewable source that can meet the demand for carbon-based liquid fuels and chemicals. The technology applied in the conversion of biomass depends on the type and complexity of the biomass, and the desired fuel. Hydrogen and hydrogen-rich mixtures (synthesis gas) are promising energy sources as they are more efficient and cleaner than existing fuels, especially when they are used in fuel cells. Hydrotreatment is a catalytic process that can be used in the conversion of biomass or biomass-derived liquids into fuels. In autothermal reforming (ATR), catalysts are used in the production of hydrogen-rich mixtures from conventional fuels or bio-fuels. The different nature of biomass and biomass-derived liquids and mineral oil makes the use of catalysts developed for the petroleum industry challenging. This requires the improvement of available catalysts and the development of new ones. To overcome the limitations of conventional hydrotreatment and ATR catalysts, zirconia-supported mono- and bimetallic rhodium, palladium, and platinum catalysts were developed and tested in the upgrading of model compounds for wood-based pyrolysis oil and in the production of hydrogen, using model compounds for gasoline and diesel. Catalysts were also tested in the ATR of ethanol. For comparative purposes commercial catalysts were tested and the results obtained with model compounds were compared with those obtained with real feedstocks (hydrotreatmet tests with wood-based pyrolysis oil and ATR tests with NExBTL renewable diesel). Noble metal catalysts were active and selective in the hydrotreatment of guaiacol used as the model compound for the lignin fraction of wood-based pyrolysis oil and wood

  1. Engineering Escherichia Coli Fatty Acid Metabolism for the Production of Biofuel Precursors

    OpenAIRE

    Ford, Tyler John

    2015-01-01

    Medium chain fatty acids (MCFAs, 6-12 carbons) are potential precursors to biofuels with properties similar to gasoline and diesel fuel but are not native products of Escherichia coli fatty acid synthesis. Herein we engineer E. coli to produce, metabolize, and activate MCFAs for their future reduction into alcohols and alkanes (potential biofuels). We develop an E. coli strain with an octanoate (8-carbon MCFA) producing enzyme (a thioesterase), metabolic knockouts, and the capa...

  2. New Biofuel Alternatives: Integrating Waste Management and Single Cell Oil Production

    OpenAIRE

    Elia Judith Martínez; Vijaya Raghavan; Fernando González-Andrés; Xiomar Gómez

    2015-01-01

    Concerns about greenhouse gas emissions have increased research efforts into alternatives in bio-based processes. With regard to transport fuel, bioethanol and biodiesel are still the main biofuels used. It is expected that future production of these biofuels will be based on processes using either non-food competing biomasses, or characterised by low CO2 emissions. Many microorganisms, such as microalgae, yeast, bacteria and fungi, have the ability to accumulate oils under special culture co...

  3. Application of experimental design methodology for optimization of biofuel production from microalgae

    International Nuclear Information System (INIS)

    Optimization of biofuel productivity, in terms of lipid content, polysaccharide content, and calorific value, from microalgae was performed by varying four variables (temperature, light intensity, nitrogen content, and CO2 addition) using a 24 full factorial design. A statistical analysis showing the influence of each variable and their interactions was conducted. The selected variables all influence biofuel productivity, but their importance varies according to the sequence: CO2 addition > temperature > nitrogen content > light intensity. Interactive effects of temperature with light intensity and nitrogen with CO2 addition for lipid and polysaccharide productivities were identified, respectively. For calorific value, interactive effects of CO2 addition with light intensity and nitrogen content were observed. The highest biofuel productivity was obtained at the following conditions: temperature (>25 °C), light intensity (>60 μmol photons m−2 s−1), nitrogen content (<50 mg L−1), and CO2 addition (>18 mL L−1 d−1). 10 days was found to be the most favorable cultivation time for lipid production under the investigated conditions. - Highlights: • Several factors influence biofuel (lipid and carbohydrate content) of microalgae. • A 24 full factorial design was used to optimize biofuel productivity. • Factor importance varies: CO2 conc. > temp. > nitrogen content > light intensity. • Interactive effects of factors for biofuel productivities were identified. • Highest biofuel productivity was obtained at above 25 °C, 60 μmol photons m−2 s−1, 18 mL CO2 L−1 d−1, and below 50 mg N L−1

  4. Methodology for calculation of carbon balances for biofuel crops production

    Science.gov (United States)

    Gerlfand, I.; Hamilton, S. K.; Snapp, S. S.; Robertson, G. P.

    2012-04-01

    Understanding the carbon balance implications for different biofuel crop production systems is important for the development of decision making tools and policies. We present here a detailed methodology for assessing carbon balances in agricultural and natural ecosystems. We use 20 years of data from Long-term Ecological Research (LTER) experiments at the Kellogg Biological Station (KBS), combined with models to produce farm level CO2 balances for different management practices. We compared four grain and one forage systems in the U.S. Midwest: corn (Zea mays) - soybean (Glycine max) - wheat (Triticum aestivum) rotations managed with (1) conventional tillage, (2) no till, (3) low chemical input, and (4) biologically-based (organic) practices; and (5) continuous alfalfa (Medicago sativa). In addition we use an abandoned agricultural field (successionnal ecosystem) as reference system. Measurements include fluxes of N2O and CH4, soil organic carbon change, agricultural yields, and agricultural inputs (e.g. fertilization and farm fuel use). In addition to measurements, we model carbon offsets associated with the use of bioenergy from agriculturally produced crops. Our analysis shows the importance of establishing appropriate system boundaries for carbon balance calculations. We explore how different assumptions regarding production methods and emission factors affect overall conclusions on carbon balances of different agricultural systems. Our results show management practices that have major the most important effects on carbon balances. Overall, agricultural management with conventional tillage was found to be a net CO2 source to the atmosphere, while agricultural management under reduced tillage, low input, or organic management sequestered carbon at rates of 93, -23, -51, and -14 g CO2e m-2 yr-1, respectively for conventionally tilled, no-till, low-input, and organically managed ecosystems. Perennial systems (alfalfa and the successionnal fields) showed net carbon

  5. Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms.

    Science.gov (United States)

    Cray, Jonathan A; Stevenson, Andrew; Ball, Philip; Bankar, Sandip B; Eleutherio, Elis C A; Ezeji, Thaddeus C; Singhal, Rekha S; Thevelein, Johan M; Timson, David J; Hallsworth, John E

    2015-06-01

    Fermentation products can chaotropically disorder macromolecular systems and induce oxidative stress, thus inhibiting biofuel production. Recently, the chaotropic activities of ethanol, butanol and vanillin have been quantified (5.93, 37.4, 174kJ kg(-1)m(-1) respectively). Use of low temperatures and/or stabilizing (kosmotropic) substances, and other approaches, can reduce, neutralize or circumvent product-chaotropicity. However, there may be limits to the alcohol concentrations that cells can tolerate; e.g. for ethanol tolerance in the most robust Saccharomyces cerevisiae strains, these are close to both the solubility limit (product formation and yields, and also therefore in the economics of biofuel production. PMID:25841213

  6. Estimation of Economic Impacts of Cellulosic Biofuel Production: A Comparative Analysis of Three Biofuel Pathways

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yimin; Goldberg, Marshall; Tan, Eric; Meyer, Pimphan A.

    2016-03-07

    The development of a cellulosic biofuel industry utilizing domestic biomass resources is expected to create opportunities for economic growth resulting from the construction and operation of new biorefineries. We applied an economic input-output model to estimate potential economic impacts, particularly gross job growth, resulting from the construction and operation of biorefineries using three different technology pathways: 1) cellulosic ethanol via biochemical conversion in Iowa, 2) renewable diesel blendstock via biological conversion in Georgia, and 3) renewable diesel and gasoline blendstock via fast pyrolysis in Mississippi. Combining direct, indirect, and induced effects, capital investment associated with the construction of a biorefinery processing 2,000 dry metric tons of biomass per day (DMT/day) could yield between 5,960 and 8,470 full-time equivalent (FTE) jobs during the construction period. Fast pyrolysis biorefineries produce the most jobs on a project level thanks to the highest capital requirement among the three pathways. Normalized for one million dollars of capital investment, the fast pyrolysis biorefineries are estimated to yield slighter more jobs (12.1 jobs) than the renewable diesel (11.8 jobs) and the cellulosic ethanol (11.6 jobs) biorefineries. While operating biorefineries is not labor-intensive, the annual operation of a 2,000 DMT/day biorefinery could support between 720 and 970 jobs when the direct, indirect, and induced effects are considered. The major factor, which results in the variations among the three pathways, is the type of biomass feedstock used for biofuels. The agriculture/forest, services, and trade industries are the primary sectors that will benefit from the ongoing operation of biorefineries.

  7. Process energy comparison for the production and harvesting of algal biomass as a biofuel feedstock.

    Science.gov (United States)

    Weschler, Matthew K; Barr, William J; Harper, Willie F; Landis, Amy E

    2014-02-01

    Harvesting and drying are often described as the most energy intensive stages of microalgal biofuel production. This study analyzes two cultivation and eleven harvest technologies for the production of microalgae biomass with and without the use of drying. These technologies were combined to form 122 different production scenarios. The results of this study present a calculation methodology and optimization of total energy demand for the production of algal biomass for biofuel production. The energetic interaction between unit processes and total process energy demand are compared for each scenario. Energy requirements are shown to be highly dependent on final mass concentration, with thermal drying being the largest energy consumer. Scenarios that omit thermal drying in favor of lipid extraction from wet biomass show the most promise for energy efficient biofuel production. Scenarios which used open ponds for cultivation, followed by settling and membrane filtration were the most energy efficient.

  8. Lifecycle Assessment of Biofuel Production from Wood Pyrolysis Technology

    Science.gov (United States)

    Manyele, S. V.

    2007-01-01

    Due to a stronger dependency on biomass for energy, there is a need for improved technologies in biomass-to-energy conversion in Tanzania. This paper presents a life cycle assessment (LCA) of pyrolysis technology used for conversion of wood and wood waste to liquid biofuel. In particular, a survey of environmental impacts of the process is…

  9. EFFECTS OF INITIAL MOISTURE CONTENT ON THE PRODUCTION AND QUALITY PROPERTIES OF SOLID BIOFUEL

    Directory of Open Access Journals (Sweden)

    Miloš Matúš

    2015-10-01

    Full Text Available The moisture content of densified biomass is a limit parameter influencing the quality of the solid biofuel. It influences its calorific value, density, mechanical strength and dimensional stability as well as the production process of this biofuel. The paper deals with the experimental research of the effect of moisture content of densified material on the final quality of biofuel in the form of logs. Experiments based on the single-axis densification of spruce sawdust were realized by hydraulic piston press, where the densified logs were produced under room temperature. The effect of moisture content on the quality properties of the logs, including density, change of moisture, expansion and physical changes, were studied. The results show the necessary moisture ranges for producing good-quality logs. The experiments were evaluated and the moisture content of the tested material was optimized to achieve the optimum value for the best quality of the solid biofuel.

  10. Panorama 2007: Potential biomass mobilization for bio-fuel production worldwide, in Europe and in France

    International Nuclear Information System (INIS)

    One key factor in ensuring the success of bio-fuel technologies, which are expected to see high growth, is the availability of biomass resources. Although the targets set in Europe and France for the replacement of petroleum products in the transport sector by 2010 can be met by converting farm surpluses into biofuels, in order to proceed further, it will be necessary to mobilize a resource that is more abundant and potentially less costly: ligno-cellulosic materials, i.e. wood or straw. The future of biofuels depends on establishing the much-awaited 'second generation' bio-fuel pathways able to convert ligno-cellulosic materials to ethanol, bio-diesel and bio-kerosene. (author)

  11. Sustainability of algal biofuel production using integrated renewable energy park (IREP) and algal biorefinery approach

    International Nuclear Information System (INIS)

    Algal biomass can provide viable third generation feedstock for liquid transportation fuel. However, for a mature commercial industry to develop, sustainability as well as technological and economic issues pertinent to algal biofuel sector must be addressed first. This viewpoint focuses on three integrated approaches laid out to meet these challenges. Firstly, an integrated algal biorefinery for sequential biomass processing for multiple high-value products is delineated to bring in the financial sustainability to the algal biofuel production units. Secondly, an integrated renewable energy park (IREP) approach is proposed for amalgamating various renewable energy industries established in different locations. This would aid in synergistic and efficient electricity and liquid biofuel production with zero net carbon emissions while obviating numerous sustainability issues such as productive usage of agricultural land, water, and fossil fuel usage. A 'renewable energy corridor' rich in multiple energy sources needed for algal biofuel production for deploying IREPs in the United States is also illustrated. Finally, the integration of various industries with algal biofuel sector can bring a multitude of sustainable deliverables to society, such as renewable supply of cheap protein supplements, health products and aquafeed ingredients. The benefits, challenges, and policy needs of the IREP approach are also discussed.

  12. Determining the global maximum biofuel production potential without conflicting with food and feed consumption

    Science.gov (United States)

    Pumkaew, Watcharapol

    This study tries to resolve the competition between food and biofuel by balancing the allocation between food and feed areas and biofuel areas for the entire world. The maximum energy production is calculated by determining the theoretical amount of energy that can be grown, once food and feed consumption is taken into account, based on the assumption that unprotected grass and woody lands and forest lands can be converted into cultivated lands. The total optimum land area for biofuel energy, 4,926.49 Mha, consists of corn, rapeseed, sugar beet, sugar cane, and grasses. When considering energy conversion efficiency, the maximum energy production is 520.5 EJ. Of this amount, 5.9 EJ can be identified with food and feed energy and 514.6 EJ can be identified with biofuel energy. This result is a theoretical value to illustrate the potential global land area for biofuel. The biofuel energy production per area of land in this study is calculated to be 0.12 EJ/Mha. With regards to the limitation in the degree of invasion by grass and woody land and forest land areas, if it is not more than 10 percent, the biofuel energy production can serve about 76 percent of energy demand for transportation in 2009. The total optimum land area is about 45 percent of global cultivated land area. Sensitivity analysis shows that the land area of corn, sweet sorghum, sugarcane, grass, and woody crops is sensitive to energy content. The land area of sweet sorghum and soybeans is sensitive to the land area for food and feed consumption. Also, the land area of corn, sugar beet, and sugarcane is sensitive to the potential crop land area. This study, done at the global level, can also apply in a local area by using local constraints.

  13. Advanced Production Planning Models

    Energy Technology Data Exchange (ETDEWEB)

    JONES,DEAN A.; LAWTON,CRAIG R.; KJELDGAARD,EDWIN A.; WRIGHT,STEPHEN TROY; TURNQUIST,MARK A.; NOZICK,LINDA K.; LIST,GEORGE F.

    2000-12-01

    >This report describes the innovative modeling approach developed as a result of a 3-year Laboratory Directed Research and Development project. The overall goal of this project was to provide an effective suite of solvers for advanced production planning at facilities in the nuclear weapons complex (NWC). We focused our development activities on problems related to operations at the DOE's Pantex Plant. These types of scheduling problems appear in many contexts other than Pantex--both within the NWC (e.g., Neutron Generators) and in other commercial manufacturing settings. We successfully developed an innovative and effective solution strategy for these types of problems. We have tested this approach on actual data from Pantex, and from Org. 14000 (Neutron Generator production). This report focuses on the mathematical representation of the modeling approach and presents three representative studies using Pantex data. Results associated with the Neutron Generator facility will be published in a subsequent SAND report. The approach to task-based scheduling described here represents a significant addition to the literature for large-scale, realistic scheduling problems in a variety of production settings.

  14. Biofuel Production by Fermentation of Water Plants and Agricultural Lignocellulosic by-Products

    Directory of Open Access Journals (Sweden)

    Anker Yaakov

    2016-01-01

    Full Text Available While at present most energy crops are depriving human feedstock, fermentation of agricultural residues and fast growing water plants possesses a good prospect to become a significant source for bio-fuel; as both substrates are widely available and do not require agricultural areas. Water hyacinth for instance can be cultivated in fresh, brackish or wastewater and owing to its rapid growth and availability. Since owing to its natural abundance it is considered to be an invasive plant in most continents, its utilization and use as a renewable energy source may also contribute for its dilution and control. Agricultural lignocellulosic surplus by-products are also a promising fermentable substrate for bioethanol production, as it decreases both disposal expenses and greenhouse gases emissions. This paper describes a scheme and methodology for transformation of any lignocellulosic biomass into biofuel by simple cost effective operation scheme, integrating an innovative process of mechanochemical activation pre-treatment followed by fermentation of the herbal digest and ethanol production through differential distillation. Under this approach several complex and costly staged of conventional ethanol production scheme may be replaced and by genetic engineering of custom fermenting microorganisms the fermentation process becomes a fully continuous industrial process.

  15. Alternative Technologies for Biofuels Production in Kraft Pulp Mills—Potential and Prospects

    Directory of Open Access Journals (Sweden)

    Esa Vakkilainen

    2012-07-01

    Full Text Available The current global conditions provide the pulp mill new opportunities beyond the traditional production of cellulose. Due to stricter environmental regulations, volatility of oil price, energy policies and also the global competitiveness, the challenges for the pulp industry are many. They range from replacing fossil fuels with renewable energy sources to the export of biofuels, chemicals and biomaterials through the implementation of biorefineries. In spite of the enhanced maturity of various bio and thermo-chemical conversion processes, the economic viability becomes an impediment when considering the effective implementation on an industrial scale. In the case of kraft pulp mills, favorable conditions for biofuels production can be created due to the availability of wood residues and generation of black liquor. The objective of this article is to give an overview of the technologies related to the production of alternative biofuels in the kraft pulp mills and discuss their potential and prospects in the present and future scenario.

  16. Biofuels production for smallholder producers in the Greater Mekong Sub-region

    Energy Technology Data Exchange (ETDEWEB)

    Malik, Urooj S.; Ahmed, Mahfuz [Southeast Asia Department, Asian Development Bank, 6 ADB Avenue, Mandaluyong City 1550 (Philippines); Sombilla, Mercedita A. [Southeast Asian Center for Graduate Studies and Research in Agriculture (SEARCA), Consulting Services Department, 4031 College, Laguna (Philippines); Cueno, Sarah L. [Agricultural Economist and Regional Program Coordinator Greater Mekong Subregion Economic Cooperation Program Working Group on Agriculture, Southeast Asia Department, Asian Development Bank, 6 ADB Avenue, Mandaluyong City 1550 (Philippines)

    2009-11-15

    Looming concerns on rising food prices and food security has slowed down the impetus in biofuel production. The development of the sub-sector, however, remains an important agenda among developing countries like those of the Greater Mekong Sub-region (GMS) that have abundant labour and natural resources but have limited supply of fossil fuels which continues to serve as a constraint to economic growth. Five crops have been selected to be further developed and use for biofuel production in the GMS, namely sugarcane, cassava, oil palm, sweet sorghum and Jathropa curcas. The expanded use of sugarcane, cassava, and oil palm for biofuel production can cause problems in the food sector. The other two crops, sweet sorghum and J. curcas, are non-food crops but could still compete with the food crops in terms of resource use for production. In all cases, the GMS needs to formulate a sustainable strategy for the biofuel development that will not compete with the food sector but will rather help achieve energy security, promote rural development and protect the environment. Except for People's Republic of China (PRC) and Thailand that already have fairly developed biofuel sub-sector, the other GMS countries are either poised to start (Lao PDR and Cambodia) or ready to enhance existing initiatives on biofuel production (Myanmar and Vietnam), with support from their respective governments. Biofuel development in these countries has to be strongly integrated with smallholder producers in order to have an impact on improving livelihood. At this initial stage, the sub-sector does not need to compete on a price basis but should rather aim to put up small-scale biofuel processing plants in remote rural areas that can offer an alternative to high-priced diesel and kerosene for local electricity grids serving homes and small enterprises. The social and economic multiplier effects are expected to be high when farmers that produce the energy crops also produce the biofuels to

  17. Biofuels production for smallholder producers in the Greater Mekong Sub-region

    International Nuclear Information System (INIS)

    Looming concerns on rising food prices and food security has slowed down the impetus in biofuel production. The development of the sub-sector, however, remains an important agenda among developing countries like those of the Greater Mekong Sub-region (GMS) that have abundant labour and natural resources but have limited supply of fossil fuels which continues to serve as a constraint to economic growth. Five crops have been selected to be further developed and use for biofuel production in the GMS, namely sugarcane, cassava, oil palm, sweet sorghum and Jathropa curcas. The expanded use of sugarcane, cassava, and oil palm for biofuel production can cause problems in the food sector. The other two crops, sweet sorghum and J. curcas, are non-food crops but could still compete with the food crops in terms of resource use for production. In all cases, the GMS needs to formulate a sustainable strategy for the biofuel development that will not compete with the food sector but will rather help achieve energy security, promote rural development and protect the environment. Except for People's Republic of China (PRC) and Thailand that already have fairly developed biofuel sub-sector, the other GMS countries are either poised to start (Lao PDR and Cambodia) or ready to enhance existing initiatives on biofuel production (Myanmar and Vietnam), with support from their respective governments. Biofuel development in these countries has to be strongly integrated with smallholder producers in order to have an impact on improving livelihood. At this initial stage, the sub-sector does not need to compete on a price basis but should rather aim to put up small-scale biofuel processing plants in remote rural areas that can offer an alternative to high-priced diesel and kerosene for local electricity grids serving homes and small enterprises. The social and economic multiplier effects are expected to be high when farmers that produce the energy crops also produce the biofuels to generate

  18. Zymomonas mobilis as a Model System for Production of Biofuels and Biochemicals

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Shihui; Fei, Qiang; Zhang, Yaoping; Contreras, Lydia M.; Utturkar, Sagar M.; Brown, Steven D.; Himmel, Michael E.; Zhang, Min

    2016-11-01

    Zymomonas mobilis is a natural ethanologen with many desirable industrial biocatalyst characteristics. In this review, we will discuss work to develop Z. mobilis as a model system for biofuel production from the perspectives of substrate utilization, development for industrial robustness, potential product spectrum, strain evaluation and fermentation strategies. This review also encompasses perspectives related to classical genetic tools and emerging technologies in this context.

  19. Development of optimal enzymatic and microbial conversion systems for biofuel production

    Science.gov (United States)

    Aramrueang, Natthiporn

    The increase in demand for fuels, along with the concerns over the depletion of fossil fuels and the environmental problems associated with the use of the petroleum-based fuels, has driven the exploitation of clean and renewable energy. Through a collaboration project with Mendota Bioenergy LLC to produce advanced biofuel from sugar beet and other locally grown crops in the Central Valley of California through demonstration and commercial-scale biorefineries, the present study focused on the investigation of selected potential biomass as biofuel feedstock and development of bioconversion systems for sustainable biofuel production. For an efficient biomass-to-biofuel conversion process, three important steps, which are central to this research, must be considered: feedstock characterization, enzymatic hydrolysis of the feedstock, and the bioconversion process. The first part of the research focused on the characterization of various lignocellulosic biomass as feedstocks and investigated their potential ethanol yields. Physical characteristics and chemical composition were analyzed for four sugar beet varieties, three melon varieties, tomato, Jose tall wheatgrass, wheat hay, and wheat straw. Melons and tomato are those products discarded by the growers or processors due to poor quality. The mass-based ethanol potential of each feedstock was determined based on the composition. The high sugar-containing feedstocks are sugar beet roots, melons, and tomato, containing 72%, 63%, and 42% average soluble sugars on a dry basis, respectively. Thus, for these crops, the soluble sugars are the main substrate for ethanol production. The potential ethanol yields, on average, for sugar beet roots, melons, and tomato are 591, 526, and 448 L ethanol/metric ton dry basis (d.b.), respectively. Lignocellulosic biomass, including Jose Tall wheatgrass and wheat straw, are composed primarily of cellulose (27-39% d.b.) and hemicellulose (26-30% d.b.). The ethanol yields from these

  20. Biofuel Production in Ireland—An Approach to 2020 Targets with a Focus on Algal Biomass

    Directory of Open Access Journals (Sweden)

    Fionnuala Murphy

    2013-12-01

    Full Text Available Under the Biofuels Obligation Scheme in Ireland, the biofuels penetration rate target for 2013 was set at 6% by volume from a previous 4% from 2010. In 2012 the fuel blend reached 3%, with approximately 70 million L of biodiesel and 56 million L of ethanol blended with diesel and gasoline, respectively. Up to and including April 2013, the current blend rate in Ireland for biodiesel was 2.3% and for bioethanol was 3.7% which equates to approximately 37.5 million L of biofuel for the first four months of 2013. The target of 10% by 2020 remains, which equates to approximately 420 million L yr−1. Achieving the biofuels target would require 345 ktoe by 2020 (14,400 TJ. Utilizing the indigenous biofuels in Ireland such as tallow, used cooking oil and oil seed rape leaves a shortfall of approximately 12,000 TJ or 350 million L (achieving only 17% of the 10% target that must be either be imported or met by other renewables. Other solutions seem to suggest that microalgae (for biodiesel and macroalgae (for bioethanol could meet this shortfall for indigenous Irish production. This paper aims to review the characteristics of algae for biofuel production based on oil yields, cultivation, harvesting, processing and finally in terms of the European Union (EU biofuels sustainability criteria, where, up to 2017, a 35% greenhouse gas (GHG emissions reduction is required compared to fossil fuels. From 2017 onwards, a 50% GHG reduction is required for existing installations and from 2018, a 60% reduction for new installations is required.

  1. Fuelling the future: microbial engineering for the production of sustainable biofuels.

    Science.gov (United States)

    Liao, James C; Mi, Luo; Pontrelli, Sammy; Luo, Shanshan

    2016-04-01

    Global climate change linked to the accumulation of greenhouse gases has caused concerns regarding the use of fossil fuels as the major energy source. To mitigate climate change while keeping energy supply sustainable, one solution is to rely on the ability of microorganisms to use renewable resources for biofuel synthesis. In this Review, we discuss how microorganisms can be explored for the production of next-generation biofuels, based on the ability of bacteria and fungi to use lignocellulose; through direct CO2 conversion by microalgae; using lithoautotrophs driven by solar electricity; or through the capacity of microorganisms to use methane generated from landfill. Furthermore, we discuss how to direct these substrates to the biosynthetic pathways of various fuel compounds and how to optimize biofuel production by engineering fuel pathways and central metabolism.

  2. Fuelling the future: microbial engineering for the production of sustainable biofuels.

    Science.gov (United States)

    Liao, James C; Mi, Luo; Pontrelli, Sammy; Luo, Shanshan

    2016-04-01

    Global climate change linked to the accumulation of greenhouse gases has caused concerns regarding the use of fossil fuels as the major energy source. To mitigate climate change while keeping energy supply sustainable, one solution is to rely on the ability of microorganisms to use renewable resources for biofuel synthesis. In this Review, we discuss how microorganisms can be explored for the production of next-generation biofuels, based on the ability of bacteria and fungi to use lignocellulose; through direct CO2 conversion by microalgae; using lithoautotrophs driven by solar electricity; or through the capacity of microorganisms to use methane generated from landfill. Furthermore, we discuss how to direct these substrates to the biosynthetic pathways of various fuel compounds and how to optimize biofuel production by engineering fuel pathways and central metabolism. PMID:27026253

  3. Application of orange peel waste in the production of solid biofuels and biosorbents.

    Science.gov (United States)

    Santos, Carolina Monteiro; Dweck, Jo; Viotto, Renata Silva; Rosa, André Henrique; de Morais, Leandro Cardoso

    2015-11-01

    This work aimed to study the potential use of pyrolyzed orange peels as solid biofuels and biosorption of heavy metals. The dry biomass and the biofuel showed moderate levels of carbon (44-62%), high levels of oxygen (30-47%), lower levels of hydrogen (3-6%), nitrogen (1-2.6%), sulfur (0.4-0.8%) and ash with a maximum of 7.8%. The activation energy was calculated using Kissinger method, involving a 3 step process: volatilization of water, biomass degradation and volatilization of the degradation products. The calorific value obtained was 19.3MJ/kg. The studies of metal biosorption based on the Langmuir model obtained the best possible data fits. The results obtained in this work indicated that the potential use of waste orange peel as a biosorbent and as a solid biofuel are feasible, this product could be used in industrial processes, favoring the world economy. PMID:26280099

  4. Microbial conversion of pyrolytic products to biofuels: a novel and sustainable approach toward second-generation biofuels.

    Science.gov (United States)

    Islam, Zia Ul; Zhisheng, Yu; Hassan, El Barbary; Dongdong, Chang; Hongxun, Zhang

    2015-12-01

    This review highlights the potential of the pyrolysis-based biofuels production, bio-ethanol in particular, and lipid in general as an alternative and sustainable solution for the rising environmental concerns and rapidly depleting natural fuel resources. Levoglucosan (1,6-anhydrous-β-D-glucopyranose) is the major anhydrosugar compound resulting from the degradation of cellulose during the fast pyrolysis process of biomass and thus the most attractive fermentation substrate in the bio-oil. The challenges for pyrolysis-based biorefineries are the inefficient detoxification strategies, and the lack of naturally available efficient and suitable fermentation organisms that could ferment the levoglucosan directly into bio-ethanol. In case of indirect fermentation, acid hydrolysis is used to convert levoglucosan into glucose and subsequently to ethanol and lipids via fermentation biocatalysts, however the presence of fermentation inhibitors poses a big hurdle to successful fermentation relative to pure glucose. Among the detoxification strategies studied so far, over-liming, extraction with solvents like (n-butanol, ethyl acetate), and activated carbon seem very promising, but still further research is required for the optimization of existing detoxification strategies as well as developing new ones. In order to make the pyrolysis-based biofuel production a more efficient as well as cost-effective process, direct fermentation of pyrolysis oil-associated fermentable sugars, especially levoglucosan is highlly desirable. This can be achieved either by expanding the search to identify naturally available direct levoglusoan utilizers or modify the existing fermentation biocatalysts (yeasts and bacteria) with direct levoglucosan pathway coupled with tolerance engineering could significantly improve the overall performance of these microorganisms.

  5. Microbial conversion of pyrolytic products to biofuels: a novel and sustainable approach toward second-generation biofuels.

    Science.gov (United States)

    Islam, Zia Ul; Zhisheng, Yu; Hassan, El Barbary; Dongdong, Chang; Hongxun, Zhang

    2015-12-01

    This review highlights the potential of the pyrolysis-based biofuels production, bio-ethanol in particular, and lipid in general as an alternative and sustainable solution for the rising environmental concerns and rapidly depleting natural fuel resources. Levoglucosan (1,6-anhydrous-β-D-glucopyranose) is the major anhydrosugar compound resulting from the degradation of cellulose during the fast pyrolysis process of biomass and thus the most attractive fermentation substrate in the bio-oil. The challenges for pyrolysis-based biorefineries are the inefficient detoxification strategies, and the lack of naturally available efficient and suitable fermentation organisms that could ferment the levoglucosan directly into bio-ethanol. In case of indirect fermentation, acid hydrolysis is used to convert levoglucosan into glucose and subsequently to ethanol and lipids via fermentation biocatalysts, however the presence of fermentation inhibitors poses a big hurdle to successful fermentation relative to pure glucose. Among the detoxification strategies studied so far, over-liming, extraction with solvents like (n-butanol, ethyl acetate), and activated carbon seem very promising, but still further research is required for the optimization of existing detoxification strategies as well as developing new ones. In order to make the pyrolysis-based biofuel production a more efficient as well as cost-effective process, direct fermentation of pyrolysis oil-associated fermentable sugars, especially levoglucosan is highlly desirable. This can be achieved either by expanding the search to identify naturally available direct levoglusoan utilizers or modify the existing fermentation biocatalysts (yeasts and bacteria) with direct levoglucosan pathway coupled with tolerance engineering could significantly improve the overall performance of these microorganisms. PMID:26433384

  6. Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose.

    Science.gov (United States)

    Yang, Xiaorui; Xu, Mengmeng; Yang, Shang-Tian

    2015-11-01

    Production of cellulosic biofuels has drawn increasing attention. However, currently no microorganism can produce biofuels, particularly butanol, directly from cellulosic biomass efficiently. Here we engineered a cellulolytic bacterium, Clostridium cellulovorans, for n-butanol and ethanol production directly from cellulose by introducing an aldehyde/alcohol dehydrogenase (adhE2), which converts butyryl-CoA to n-butanol and acetyl-CoA to ethanol. The engineered strain was able to produce 1.42 g/L n-butanol and 1.60 g/L ethanol directly from cellulose. Moreover, the addition of methyl viologen as an artificial electron carrier shifted the metabolic flux from acid production to alcohol production, resulting in a high biofuel yield of 0.39 g/g from cellulose, comparable to ethanol yield from corn dextrose by yeast fermentation. This study is the first metabolic engineering of C. cellulovorans for n-butanol and ethanol production directly from cellulose with significant titers and yields, providing a promising consolidated bioprocessing (CBP) platform for biofuel production from cellulosic biomass.

  7. Assessing the Environmental Performance of Integrated Ethanol and Biogas Production: : Quantifying Industrial Symbiosis in the Biofuel Industry

    OpenAIRE

    Martin, Michael; Svensson, Niclas; Jorge FONSECA

    2011-01-01

    As the production of biofuels continues to expand worldwide, criticism about many issues, including the energy output versus input and the competition with food, has been raised andthe sustainability of biofuels in recent years has been constantly debated. However, the current biofuel systems may be optimized to increase the energy efficiency and environmentalperformance. By using concepts from industrial symbiosis, the material and energy exchangesmay be optimized to result in these performa...

  8. Advanced biofuels - GHG emissions and energy balances. A report to IEA bioenergy task 39

    Energy Technology Data Exchange (ETDEWEB)

    O' Connor, Don [S and T 2 Consultants Inc., Delta, British Columbia (Canada)

    2013-05-25

    In this work, a number of advanced biofuel pathways were examined with respect to their energy balances and GHG emission performance. Some of these pathways have relatively detailed public techno-economic modelling studies available on which the energy and GHG lifecycle modelling has been based. However there is a continuum in the quality of publicly available data and, for some of the pathways a significant number of assumptions had to be made in order to generate results. Some caution is therefore warranted when the results of different systems are compared. Furthermore, none of the modelling data is based on actual operating systems, as the processes being assessed are not yet in commercial operation; rather, they are each in different stages of research, development and demonstration.

  9. A thermophilic ionic liquid-tolerant cellulase cocktail for the production of cellulosic biofuels.

    Directory of Open Access Journals (Sweden)

    Joshua I Park

    Full Text Available Generation of biofuels from sugars in lignocellulosic biomass is a promising alternative to liquid fossil fuels, but efficient and inexpensive bioprocessing configurations must be developed to make this technology commercially viable. One of the major barriers to commercialization is the recalcitrance of plant cell wall polysaccharides to enzymatic hydrolysis. Biomass pretreatment with ionic liquids (ILs enables efficient saccharification of biomass, but residual ILs inhibit both saccharification and microbial fuel production, requiring extensive washing after IL pretreatment. Pretreatment itself can also produce biomass-derived inhibitory compounds that reduce microbial fuel production. Therefore, there are multiple points in the process from biomass to biofuel production that must be interrogated and optimized to maximize fuel production. Here, we report the development of an IL-tolerant cellulase cocktail by combining thermophilic bacterial glycoside hydrolases produced by a mixed consortia with recombinant glycoside hydrolases. This enzymatic cocktail saccharifies IL-pretreated biomass at higher temperatures and in the presence of much higher IL concentrations than commercial fungal cocktails. Sugars obtained from saccharification of IL-pretreated switchgrass using this cocktail can be converted into biodiesel (fatty acid ethyl-esters or FAEEs by a metabolically engineered strain of E. coli. During these studies, we found that this biodiesel-producing E. coli strain was sensitive to ILs and inhibitors released by saccharification. This cocktail will enable the development of novel biomass to biofuel bioprocessing configurations that may overcome some of the barriers to production of inexpensive cellulosic biofuels.

  10. Soil water infiltration affected by biofuel and grain crop production systems in claypan landscape

    Science.gov (United States)

    The effect of soil management systems on water infiltration is very crucial within claypan landscapes to maximize production as well as minimize environmental risks. The objective of this study was to assess the effect of topsoil thickness on water infiltration in claypan soils for grain and biofuel...

  11. The economics of cyanobacteria-based biofuel production: challenges and opportunities

    NARCIS (Netherlands)

    N.K. Sharma; L.J. Stal

    2014-01-01

    In the current scenario, biofuels based on algae, including cyanobacteria, are expensive, complex to produce, and are only just entering the commercial phase in small quantities in pilot or demonstration plants. This chapter discusses the current scenario of using cyanobacteria for the production of

  12. Biomass pyrolysis as an alternative process for the production of 2nd generation biofuels

    NARCIS (Netherlands)

    Kogdenko, Nadezda

    2010-01-01

    Bio-fuel production from renewable energy sources is the topic that have been studied by scientists and discussed in a political agenda for a couple of decades. In this period of time, however, it was discovered that approaches and technologies used until

  13. Small Review: Strategies for Palm Kernel Cake (PKC As a New Potential Substrate in Biofuel Production

    Directory of Open Access Journals (Sweden)

    Hafiza Shukor

    2013-01-01

    Full Text Available The economic dependency on fossil fuels and the resulting effects on climate and environment have put tremendous focus on utilizing fermentable sugars from lignocellulose, the largest known renewable carbohydrate source. Palm kernel cake (PKC is a residue from palm oil extraction presently only used as a low protein feed supplement. It’s contains 50% fermentable hexose sugars present in the form of glucan and mainly galactomannan. This makes PKC an interesting feedstock for processing into biofuel or in other biorefinery processes. This article reviews biotechnological innovation on Palm Kernel Cake (PKC as new potential of fermentable sugar for biofuel production. Strategies for biofuel production by utilizing palm kernel cake by several pretreatment processes to convert glucan and especially galactomanan into fermentable hexose sugar and further requirements to make fermentative biofuel production a successful industrial process are also discussed. This material recovery especially from lignocellulose agricultural wastes by product of palm oil mill industry into this potential bioproducts has not only benefited in oil palm planted but also to the environment and helps preserve natural resource.

  14. Biofuels from food processing wastes.

    Science.gov (United States)

    Zhang, Zhanying; O'Hara, Ian M; Mundree, Sagadevan; Gao, Baoyu; Ball, Andrew S; Zhu, Nanwen; Bai, Zhihui; Jin, Bo

    2016-04-01

    Food processing industry generates substantial high organic wastes along with high energy uses. The recovery of food processing wastes as renewable energy sources represents a sustainable option for the substitution of fossil energy, contributing to the transition of food sector towards a low-carbon economy. This article reviews the latest research progress on biofuel production using food processing wastes. While extensive work on laboratory and pilot-scale biosystems for energy production has been reported, this work presents a review of advances in metabolic pathways, key technical issues and bioengineering outcomes in biofuel production from food processing wastes. Research challenges and further prospects associated with the knowledge advances and technology development of biofuel production are discussed.

  15. Biofuels from food processing wastes.

    Science.gov (United States)

    Zhang, Zhanying; O'Hara, Ian M; Mundree, Sagadevan; Gao, Baoyu; Ball, Andrew S; Zhu, Nanwen; Bai, Zhihui; Jin, Bo

    2016-04-01

    Food processing industry generates substantial high organic wastes along with high energy uses. The recovery of food processing wastes as renewable energy sources represents a sustainable option for the substitution of fossil energy, contributing to the transition of food sector towards a low-carbon economy. This article reviews the latest research progress on biofuel production using food processing wastes. While extensive work on laboratory and pilot-scale biosystems for energy production has been reported, this work presents a review of advances in metabolic pathways, key technical issues and bioengineering outcomes in biofuel production from food processing wastes. Research challenges and further prospects associated with the knowledge advances and technology development of biofuel production are discussed. PMID:26874262

  16. Panorama 2011: New bio-fuel production technologies: overview of these expanding sectors and the challenges facing them

    International Nuclear Information System (INIS)

    The numerous research programmes looking at new-generation biofuels that were initiated over the last ten years are now starting to bear fruit. Although no plants are producing and marketing biofuels yet, the large-scale, industrial feasibility of second-generation bio-fuel production at competitive cost may be demonstrated in the short-term. As far as third generation biofuels derived from algal biomass are concerned, there is a great deal of R and D interest in the sector, but the technology is still only in its infancy. (author)

  17. Trash to treasure: Production of biofuels and commodity chemicals via syngas fermenting microorganisms

    DEFF Research Database (Denmark)

    Latif, Haythem; Zeidan, Ahmad; Nielsen, Alex Toftgaard;

    2014-01-01

    Fermentation of syngas is a means through which unutilized organic waste streams can be converted biologically into biofuels and commodity chemicals. Despite recent advances, several issues remain which limit implementation of industrial-scale syngas fermentation processes. At the cellular level......, the energy conservation mechanism of syngas fermenting microorganisms has not yet been entirely elucidated. Furthermore, there was a lack of genetic tools to study and ultimately enhance their metabolic capabilities. Recently, substantial progress has been made in understanding the intricate energy...

  18. Developing Research Capabilities in Energy Biosciences: Design principles of photosynthetic biofuel production.

    Energy Technology Data Exchange (ETDEWEB)

    Donald D. Brown; David Savage

    2012-06-30

    The current fossil fuel-based energy infrastructure is not sustainable. Solar radiation is a plausible alternative, but realizing it as such will require significant technological advances in the ability to harvest light energy and convert it into suitable fuels. The biological system of photosynthesis can carry out these reactions, and in principle could be engineered using the tools of synthetic biology. One desirable implementation would be to rewire the reactions of a photosynthetic bacterium to direct the energy harvested from solar radiation into the synthesis of the biofuel H2. Proposed here is a series of experiments to lay the basic science groundwork for such an attempt. The goal is to elucidate the transcriptional network of photosynthesis using a novel driver-reporter screen, evolve more robust hydrogenases for improved catalysis, and to test the ability of the photosynthetic machinery to directly produce H2 in vivo. The results of these experiments will have broad implications for the understanding of photosynthesis, enzyme function, and the engineering of biological systems for sustainable energy production. The ultimate impact could be a fundamental transformation of the world's energy economy.

  19. Biofuels and their by-products: Global economic and environmental implications

    International Nuclear Information System (INIS)

    Recently a number of papers have used general equilibrium models to study the economy-wide and environmental consequences of the first generation of biofuels (FGB). In this paper, we argue that nearly all of these studies have overstated the impacts of FGB on global agricultural and land markets due to the fact that they have ignored the role of biofuel by-products. Feed by-products of FGB, such as dried distillers grains with solubles (DDGS) and oilseed meals (VOBP), are used in the livestock industry as protein and energy sources. Their presence mitigates the price impacts of biofuel production. More importantly, they reduce the demand for cropland and moderate the indirect land use consequences of FGB. This paper explicitly introduces DDGS and VOBP into a global computational general equilibrium (CGE) model, developed at the Center for Global Trade Analysis at Purdue University, to examine the economic and environmental impacts of regional and international mandate policies designed to stimulate bioenergy production and use. We show that models with and without by-products reveal different portraits of the economic impacts of the US and EU biofuel mandates for the world economy in 2015. While both models demonstrate significant changes in the agricultural production pattern across the world, the model with by-products shows smaller changes in the production of cereal grains and larger changes for oilseeds products in the US and EU, and the reverse for Brazil. Models that omit by-products are found to overstate cropland conversion from US and EU mandates by about 27%. (author)

  20. Advances in production technology

    CERN Document Server

    2015-01-01

    This edited volume contains the selected papers presented at the scientific board meeting of the German Cluster of Excellence on “Integrative Production Technology for High-Wage Countries”,  held in November 2014. The topical structure of the book is clustered in six sessions: Integrative Production Technology, Individualised Production, Virtual Production Systems, Integrated Technologies, Self-Optimising Production Systems and Human Factors in Production Technology. The Aachen perspective on a holistic theory of production is complemented by conference papers from external leading researchers in the fields of production, materials science and bordering disciplines. The target audience primarily comprises research experts and practitioners in the field but the book may also be beneficial for graduate students.

  1. Scope of algae as third generation biofuels

    Directory of Open Access Journals (Sweden)

    Shuvashish eBehera

    2015-02-01

    Full Text Available An initiative has been taken to develop different solid, liquid and gaseous biofuels as the alternative energy resources. The current research and technology based on the third generation biofuels derived from algal biomass have been considered as the best alternative bioresource that avoids the disadvantages of first and second generation biofuels. Algal biomass have been investigated for the implementation of economic conversion processes producing different biofuels such as biodiesel, bioethanol, biogas, biohydrogen and other valuable co-products. In the present review, the recent findings and advance developments in algal biomass for improved biofuel production. This review discusses about the importance of the algal cell contents, various strategies for product formation through various conversion technologies, and its future scope as an energy security.

  2. Methodological Foundations of Clustering and Innovativeness for Establishing the Competitive Production of Biofuels

    Directory of Open Access Journals (Sweden)

    Klymchuk Oleksandr V.

    2016-05-01

    Full Text Available The article is aimed to study the worldwide trends in development of innovative processes and creation of cluster structures for elaborating methodological foundations for establishing the competitive production of biofuels. The article highlights the cluster approaches in conducting the global commercial activities that create effective mechanisms and tools to encourage innovation-investment regional development and can be characterized by their relevance for the Ukrainian economy. Emphasis is made on the matter that clustering is one of the key tools for structuring the energy market, integrated exploiting the potential of bioenergy industry sector, management of the economic policies of redistribution of value added, implementation of the growth of investment attractiveness of the biofuel industry in our country. It has been concluded that cluster development in the biofuel production will stimulate specialization and cooperation processes in the agro-industrial economy sector, bringing together related businesses in the direction of an effective interaction, thereby ensuring a high level of competitiveness of biofuels in both the national and the international markets.

  3. Economic evaluation of technology for a new generation biofuel production using wastes.

    Science.gov (United States)

    Koutinas, Athanasios; Kanellaki, Maria; Bekatorou, Argyro; Kandylis, Panagiotis; Pissaridi, Katerina; Dima, Agapi; Boura, Konstantina; Lappa, Katerina; Tsafrakidou, Panagiota; Stergiou, Panagiota-Yiolanda; Foukis, Athanasios; Gkini, Olga A; Papamichael, Emmanuel M

    2016-01-01

    An economic evaluation of an integrated technology for industrial scale new generation biofuel production using whey, vinasse, and lignocellulosic biomass as raw materials is reported. Anaerobic packed-bed bioreactors were used for organic acids production using initially synthetic media and then wastes. Butyric, lactic and acetic acid were predominately produced from vinasse, whey, and cellulose, respectively. Mass balance was calculated for a 16,000L daily production capacity. Liquid-liquid extraction was applied for recovery of the organic acids using butanol-1 as an effective extraction solvent which serves also as the alcohol for the subsequent enzyme-catalyzed esterification. The investment needed for the installation of the factory was estimated to about 1.7million€ with depreciation excepted at about 3months. For cellulosics, the installation investment was estimated to be about 7-fold higher with depreciation at about 1.5years. The proposed technology is an alternative trend in biofuel production. PMID:26492169

  4. Characterization of residual biomass from the Arequipa region for the production of biofuels

    Directory of Open Access Journals (Sweden)

    María Laura Stronguiló Leturia

    2015-12-01

    Full Text Available The aim of this work is to select residual biomass from the Arequipa Region for the production of biofuels (biodiesel, bioethanol and biogas. In each case, the initial point is a matrix based on products with residual biomass available in the region, from the agricultural and livestock sectors, information that was obtained from the regional Management of Agriculture web site. Specific factors of the resudue that will be used as raw material for each biofuel production would be considered for the selection process. For the production of biodiesel it is necessary to start from the oil extracted from oilseeds. Regarding obtaining bioethanol, it requires that the residual biomass has high percent of cellulose. With regard to the generation of biogas, we will use animal droppings. Finally, the raw materials selected are: squash and avocado seeds for biodiesel, rice chaff and deseeded corncob for bioethanol and cow and sheep droppings for biogas

  5. Economic evaluation of technology for a new generation biofuel production using wastes.

    Science.gov (United States)

    Koutinas, Athanasios; Kanellaki, Maria; Bekatorou, Argyro; Kandylis, Panagiotis; Pissaridi, Katerina; Dima, Agapi; Boura, Konstantina; Lappa, Katerina; Tsafrakidou, Panagiota; Stergiou, Panagiota-Yiolanda; Foukis, Athanasios; Gkini, Olga A; Papamichael, Emmanuel M

    2016-01-01

    An economic evaluation of an integrated technology for industrial scale new generation biofuel production using whey, vinasse, and lignocellulosic biomass as raw materials is reported. Anaerobic packed-bed bioreactors were used for organic acids production using initially synthetic media and then wastes. Butyric, lactic and acetic acid were predominately produced from vinasse, whey, and cellulose, respectively. Mass balance was calculated for a 16,000L daily production capacity. Liquid-liquid extraction was applied for recovery of the organic acids using butanol-1 as an effective extraction solvent which serves also as the alcohol for the subsequent enzyme-catalyzed esterification. The investment needed for the installation of the factory was estimated to about 1.7million€ with depreciation excepted at about 3months. For cellulosics, the installation investment was estimated to be about 7-fold higher with depreciation at about 1.5years. The proposed technology is an alternative trend in biofuel production.

  6. Environmental, economic and social impact of aviation biofuel production in Brazil.

    Science.gov (United States)

    Cremonez, Paulo André; Feroldi, Michael; de Jesus de Oliveira, Carlos; Teleken, Joel Gustavo; Alves, Helton José; Sampaio, Silvio Cézar

    2015-03-25

    The Brazilian aviation industry is currently developing biofuel technologies that can maintain the operational and energy demands of the sector, while reducing the dependence on fossil fuels (mainly kerosene) and greenhouse gas emissions. The aim of the current research was to identify the major environmental, economic and social impacts arising from the production of aviation biofuels in Brazil. Despite the great potential of these fuels, there is a significant need for improved routes of production and specifically for lower production costs of these materials. In addition, the productive chains of raw materials for obtaining these bioenergetics can be linked to environmental impacts by NOx emissions, extensive use of agricultural land, loss of wildlife and intensive water use, as well as economic, social and political impacts.

  7. A High-Resolution National Microalgae Biofuel Production and Resource Assessment

    Science.gov (United States)

    Wigmosta, M.; Coleman, A.; Skaggs, R.; Venteris, E.

    2012-12-01

    Microalgae are receiving increased global attention as a potential sustainable "energy crop" for biofuel production. An important step to realizing the potential of algae is quantifying the demands commercial-scale algal biofuel production will place on available resources. We present a high-resolution national-scale spatiotemporal assessment that begins to answer fundamental questions of where sustainable production can occur, what types and quantities of water, land, and nutrients are required, and how much energy is produced. A series of coupled model components were developed at a high spatiotemporal scale on the basis of the dominant biophysical processes affecting algal growth. Land suitable for open pond microalgae production consisting of 1200 acres per unit farm is identified using a multi-criteria land suitability model. Physics-based biomass growth and pond temperature models are then are used with location-specific meteorological and topographic data at 89,756 suitable unit farms to estimate 30-years of hourly biofuel production, nutrient requirements, and multi-source consumptive water demand. These resource requirements are compared with available resource supply and transport constraints to prioritize potential locations for sustainable microalgae feedstock production and evaluate the associated tradeoffs between production, resources, and economics.

  8. Spatio-Temporal Impacts of Biofuel Production and Climate Variability on Water Quantity and Quality in Upper Mississippi River Basin

    Directory of Open Access Journals (Sweden)

    Debjani Deb

    2015-06-01

    Full Text Available Impact of climate change on the water resources of the United States exposes the vulnerability of feedstock-specific mandated fuel targets to extreme weather conditions that could become more frequent and intensify in the future. Consequently, a sustainable biofuel policy should consider: (a how climate change would alter both water supply and demand; and (b in turn, how related changes in water availability will impact the production of biofuel crops; and (c the environmental implications of large scale biofuel productions. Understanding the role of biofuels in the water cycle is the key to understanding many of the environmental impacts of biofuels. Therefore, the focus of this study is to model the rarely explored interactions between land use, climate change, water resources and the environment in future biofuel production systems. Results from this study will help explore the impacts of the US biofuel policy and climate change on water and agricultural resources. We used the Soil and Water Assessment Tool (SWAT to analyze the water quantity and quality consequences of land use and land management related changes in cropping conditions (e.g., more use of marginal lands, greater residue harvest, increased yields, plus management practices due to biofuel crops to meet the Renewable Fuel Standard target on water quality and quantity.

  9. The Role of Social Constructions and Biophysical Attributes of the Environment in Decision-Making in the Context of Biofuels and Rubber Production Partnership Regimes in Upland Philippines

    Science.gov (United States)

    Montefrio, M. F.

    2012-12-01

    Burgeoning attention in biofuels and natural rubber has spurred interest among governments and private companies in integrating marginalized communities into global commodity markets. Upland farmers from diverse cultural backgrounds and biophysical settings today are deciding whether to agree with partnership proposals from governments and private firms to grow biofuels and natural rubber. In this paper, I examine whether upland farmers' socio-environmental constructions (evaluative beliefs, place satisfaction, and ecological worldviews) and the actual biophysical attributes (land cover and soil types) of upland environments, respectively, function as significant predictors of the intent and decisions of indigenous and non-indigenous farmers to cooperate with government and private actors to establish certain biofuel crops and natural rubber production systems in Palawan, Philippines. Drawing from ethnography and statistical analysis of household surveys, I propose that social constructions and the biophysical attributes of the environment are closely related with each other and in turn both influence individual decision-making behavior in resource-based production partnership regimes. This has significant implications on the resilience of socio-ecological systems, particularly agro-ecosystems, as certain upland farmers prefer to engage in intensive, monocrop production of biofuels and natural rubber on relatively more biodiverse areas, such as secondary forests and traditional shifting cultivation lands. The study aims to advance new institutional theories of resource management, particularly Ostrom's Institutional Analysis and Development and Socio-Ecological Systems frameworks, and scholarship on environmental decision-making in the context of collective action.

  10. Chromatin landscaping in algae reveals novel regulation pathway for biofuels production

    Energy Technology Data Exchange (ETDEWEB)

    Ngan, Chew Yee; Wong, Chee-Hong; Choi, Cindy; Pratap, Abhishek; Han, James; Wei, Chia-Lin

    2013-02-19

    The diminishing reserve of fossil fuels calls for the development of biofuels. Biofuels are produced from renewable resources, including photosynthetic organisms, generating clean energy. Microalgae is one of the potential feedstock for biofuels production. It grows easily even in waste water, and poses no competition to agricultural crops for arable land. However, little is known about the algae lipid biosynthetic regulatory mechanisms. Most studies relied on the homology to other plant model organisms, in particular Arabidopsis or through low coverage expression analysis to identify key enzymes. This limits the discovery of new components in the biosynthetic pathways, particularly the genetic regulators and effort to maximize the production efficiency of algal biofuels. Here we report an unprecedented and de novo approach to dissect the algal lipid pathways through disclosing the temporal regulations of chromatin states during lipid biosynthesis. We have generated genome wide chromatin maps in chlamydomonas genome using ChIP-seq targeting 7 histone modifications and RNA polymerase II in a time-series manner throughout conditions activating lipid biosynthesis. To our surprise, the combinatory profiles of histone codes uncovered new regulatory mechanism in gene expression in algae. Coupled with matched RNA-seq data, chromatin changes revealed potential novel regulators and candidate genes involved in the activation of lipid accumulations. Genetic perturbation on these candidate regulators further demonstrated the potential to manipulate the regulatory cascade for lipid synthesis efficiency. Exploring epigenetic landscape in microalgae shown here provides powerful tools needed in improving biofuel production and new technology platform for renewable energy generation, global carbon management, and environmental survey.

  11. Quantifying the climate impacts of albedo changes due to biofuel production: a comparison with biogeochemical effects

    OpenAIRE

    Caiazzo, Fabio; Malina, Robert; Staples, Mark Douglas; Wolfe, Philip James; Yim, Hung Lam Steve; Barrett, Steven

    2014-01-01

    Lifecycle analysis is a tool widely used to evaluate the climate impact of greenhouse gas emissions attributable to the production and use of biofuels. In this paper we employ an augmented lifecycle framework that includes climate impacts from changes in surface albedo due to land use change. We consider eleven land-use change scenarios for the cultivation of biomass for middle distillate fuel production, and compare our results to previous estimates of lifecycle greenhouse gas emissions for ...

  12. Algal biofuels.

    Science.gov (United States)

    Razeghifard, Reza

    2013-11-01

    The world is facing energy crisis and environmental issues due to the depletion of fossil fuels and increasing CO2 concentration in the atmosphere. Growing microalgae can contribute to practical solutions for these global problems because they can harvest solar energy and capture CO2 by converting it into biofuel using photosynthesis. Microalgae are robust organisms capable of rapid growth under a variety of conditions including in open ponds or closed photobioreactors. Their reduced biomass compounds can be used as the feedstock for mass production of a variety of biofuels. As another advantage, their ability to accumulate or secrete biofuels can be controlled by changing their growth conditions or metabolic engineering. This review is aimed to highlight different forms of biofuels produced by microalgae and the approaches taken to improve their biofuel productivity. The costs for industrial-scale production of algal biofuels in open ponds or closed photobioreactors are analyzed. Different strategies for photoproduction of hydrogen by the hydrogenase enzyme of green algae are discussed. Algae are also good sources of biodiesel since some species can make large quantities of lipids as their biomass. The lipid contents for some of the best oil-producing strains of algae in optimized growth conditions are reviewed. The potential of microalgae for producing petroleum related chemicals or ready-make fuels such as bioethanol, triterpenic hydrocarbons, isobutyraldehyde, isobutanol, and isoprene from their biomass are also presented.

  13. Engineering biofuel tolerance in non-native producing microorganisms.

    Science.gov (United States)

    Jin, Hu; Chen, Lei; Wang, Jiangxin; Zhang, Weiwen

    2014-01-01

    Large-scale production of renewable biofuels through microbiological processes has drawn significant attention in recent years, mostly due to the increasing concerns on the petroleum fuel shortages and the environmental consequences of the over-utilization of petroleum-based fuels. In addition to native biofuel-producing microbes that have been employed for biofuel production for decades, recent advances in metabolic engineering and synthetic biology have made it possible to produce biofuels in several non-native biofuel-producing microorganisms. Compared to native producers, these non-native systems carry the advantages of fast growth, simple nutrient requirements, readiness for genetic modifications, and even the capability to assimilate CO2 and solar energy, making them competitive alternative systems to further decrease the biofuel production cost. However, the tolerance of these non-native microorganisms to toxic biofuels is naturally low, which has restricted the potentials of their application for high-efficiency biofuel production. To address the issues, researches have been recently conducted to explore the biofuel tolerance mechanisms and to construct robust high-tolerance strains for non-native biofuel-producing microorganisms. In this review, we critically summarize the recent progress in this area, focusing on three popular non-native biofuel-producing systems, i.e. Escherichia coli, Lactobacillus and photosynthetic cyanobacteria.

  14. BIOMASS SUPPLY FOR BIOFUEL PRODUCTION: ESTIMATES FOR THE UNITED STATES AND CANADA

    Directory of Open Access Journals (Sweden)

    Subbu Kumarappan

    2009-08-01

    Full Text Available The potential supply of biomass feedstocks in the US and Canada is estimated using a static supply function approach. Estimated total biomass available at a price of $100 per metric ton is 568 million metric tons in the US and 123 million tons in Canada, which together can displace 23-45 billion gallons of gasoline. Sufficient biomass, mainly agricultural and mill residues, will be available at prices of around $50/ton to meet the advanced biofuel mandates of the US Energy Independence and Security Act of 2007. The estimates of agricultural residue supply are very sensitive to the assumed fraction of residues that can be sustainably removed from the field, and the potential of municipal solid waste as a feedstock depends on which components can be economically converted into liquid biofuels.

  15. Life cycle assessment of energy products: environmental impact assessment of biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Zah, R.; Boeni, H.; Gauch, M.; Hischier, R.; Lehmann, M.; Waeger, P.

    2007-05-15

    This final report for the Swiss Federal Office of Energy (SFOE) deals with the results of a study that evaluated the environmental impact of the entire production chain of fuels made from biomass and used in Switzerland. Firstly, the study supplies an analysis of the possible environmental impacts of biofuels that can be used as a basis for political decisions. Secondly, an environmental life cycle assessment (LCA) of various biofuels is presented. In addition, the impacts of fuel use are compared with other uses for bioenergy such as the generation of electricity and heat. The methods used in the LCA are discussed, including the Swiss method of ecological scarcity (Environmental Impact Points, UBP 06), and the European Eco-indicator 99 method. The results of the study are discussed, including the finding that not all biofuels can reduce environmental impacts as compared to fossil fuels. The role to be played by biofuels produced in an environmentally-friendly way together with other forms of renewable energy in our future energy supply is discussed.

  16. Biofuel production potentials in Europe: Sustainable use of cultivated land and pastures. Part I: Land productivity potentials

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, Guenther; Prieler, Sylvia; van Velthuizen, Harrij [International Institute for Applied Systems Analysis (IIASA) (Austria); Lensink, Sander M.; Londo, Marc [Energy Research Institute of the Netherlands (ECN), Unit Policy Studies, Westerduinweg 3, 1755 LE Petten P.O. Box 1 (Netherlands); de Wit, Marc [Copernicus Institute for Sustainable Development, Utrecht University Heidelberglaan 2, 3584 CS Utrecht (Netherlands)

    2010-02-15

    IIASA's agro-ecological zones modelling framework has been extended for biofuel productivity assessments distinguishing five main groups of feedstocks covering a wide range of agronomic conditions and energy production pathways, namely: woody lignocellulosic plants, herbaceous lignocellulosic plants, oil crops, starch crops and sugar crops. A uniform Pan-European land resources database was compiled at the spatial resolution of 1 km{sup 2}. Suitability and productivity assessments were carried out by matching climate characteristics with plant requirements, calculating annual biomass increments or yields including consideration of soil and terrain characteristics of each grid-cell. Potential biomass productivity and associated energy yields were calculated for each grid-cell. Spatial distributions of suitabilities of biofuel feedstocks in Europe were generated for each individual feedstock as well as for the five biofuel feedstock groups. Estimated agronomical attainable yields, both in terms of biomass (kg ha{sup -1}) as well as biofuel energy equivalent (GJ ha{sup -1}), were mapped and tabulated by agriculture and pasture land cover classes as derived from the CORINE land cover database. Results have been further aggregated by administrative units at NUTS 2 level. (author)

  17. Biofuel production potentials in Europe. Sustainable use of cultivated land and pastures. Part 1. Land productivity potentials

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, G; Prieler, S.; Van Velthuizen, H. [IIASA International Institute of Applied Systems Analysis, Laxenburg (Austria); Lensink, S.M.; Londo, H.M. [ECN Policy Studies, Petten (Netherlands); De Wit, M. [Copernicus Institute, Utrecht University, Utrecht (Netherlands)

    2009-10-15

    IIASA's agro-ecological zones modelling framework has been extended for biofuel productivity assessments distinguishing five main groups of feedstocks covering a wide range of agronomic conditions and energy production pathways, namely: woody lignocellulosic plants, herbaceous lignocellulosic plants, oil crops, starch crops and sugar crops. A uniform Pan-European land resources database was compiled at the spatial resolution of 1 km{sup 2}. Suitability and productivity assessments were carried out by matching climate characteristics with plant requirements, calculating annual biomass increments or yields including consideration of soil and terrain characteristics of each grid-cell. Potential biomass productivity and associated energy yields were calculated for each gridcell. Spatial distributions of suitabilities of biofuel feedstocks in Europe were generated for each individual feedstock as well as for the five biofuel feedstock groups. Estimated agronomical attainable yields, both in terms of biomass (kg ha{sup -1}) as well as biofuel energy equivalent (GJ ha{sup -1}), were mapped and tabulated by agriculture and pasture land cover classes as derived from the CORINE land cover database. Results have been further aggregated by administrative units at NUTS 2 level.

  18. Organisms for biofuel production: natural bioresources and methodologies for improving their biosynthetic potentials.

    Science.gov (United States)

    Hu, Guangrong; Ji, Shiqi; Yu, Yanchong; Wang, Shi'an; Zhou, Gongke; Li, Fuli

    2015-01-01

    In order to relieve the pressure of energy supply and environment contamination that humans are facing, there are now intensive worldwide efforts to explore natural bioresources for production of energy storage compounds, such as lipids, alcohols, hydrocarbons, and polysaccharides. Around the world, many plants have been evaluated and developed as feedstock for bioenergy production, among which several crops have successfully achieved industrialization. Microalgae are another group of photosynthetic autotroph of interest due to their superior growth rates, relatively high photosynthetic conversion efficiencies, and vast metabolic capabilities. Heterotrophic microorganisms, such as yeast and bacteria, can utilize carbohydrates from lignocellulosic biomass directly or after pretreatment and enzymatic hydrolysis to produce liquid biofuels such as ethanol and butanol. Although finding a suitable organism for biofuel production is not easy, many naturally occurring organisms with good traits have recently been obtained. This review mainly focuses on the new organism resources discovered in the last 5 years for production of transport fuels (biodiesel, gasoline, jet fuel, and alkanes) and hydrogen, and available methods to improve natural organisms as platforms for the production of biofuels.

  19. Interactions of woody biofuel feedstock production systems with water resources: Considerations for sustainability.

    Energy Technology Data Exchange (ETDEWEB)

    Trettin, Carl,C.; Amatya, Devendra; Coleman, Mark.

    2008-07-01

    Abstract. Water resources are important for the production of woody biofuel feedstocks. It is necessary to ensure that production systems do not adversely affect the quantity or quality of surface and ground water. The effects of woody biomass plantations on water resources are largely dependent on the prior land use and the management regime. Experience from both irrigated and non-irrigated systems has demonstrated that woody biofuel production systems do not impair water quality. Water quality actually improves from conversion of idle or degraded agricultural lands to woody biomass plantations. Site water balance may be altered by cultivation of woody biomass plantations relative to agricultural use, due to increases in evapostranspiration (ET) and storage. Incorporation of woody biomass production plantations within the landscape provides an opportunity to improve the quality of runoff water and soil conservation. Given the centrality of water resources to the sustainability of ecosystem services and other values derived, the experience with woody biofuels feedstock production systems is positive. Keywords. Short rotation woody crop, forest hydrology, water quality, hardwood plantation.

  20. Which future for aviation bio-fuels?

    International Nuclear Information System (INIS)

    This collective report proposes a detailed overview of the evolution of aviation fuels and bio-fuels from technological, regulatory and economic points of view. It also proposes a road-map for possible future evolutions, and outlines the different assessments between American and European countries regarding the predictions for the beginning of industrial production and use of bio-jet-fuel. After having recalled international objectives, an overview of European and French commitments for technological and operational advances, and a discussion of the role of bio-fuels in the carbon cycle, the report presents various technical constraints met in aircraft industry and describes the role bio-fuels may have. The next part proposes an overview of bio-fuels which are industrially produced in the world in 2013. The authors then focus on aviation bio-fuels (main production processes, thermo-chemical processes), discuss the political context, and examine obstacles, partnerships and the role of public authorities

  1. Importance of systems biology in engineering microbes for biofuel production

    Energy Technology Data Exchange (ETDEWEB)

    Mukhopadhyay, Aindrila; Redding, Alyssa M.; Rutherford, Becky J.; Keasling, Jay D.

    2009-12-02

    Microorganisms have been rich sources for natural products, some of which have found use as fuels, commodity chemicals, specialty chemicals, polymers, and drugs, to name a few. The recent interest in production of transportation fuels from renewable resources has catalyzed numerous research endeavors that focus on developing microbial systems for production of such natural products. Eliminating bottlenecks in microbial metabolic pathways and alleviating the stresses due to production of these chemicals are crucial in the generation of robust and efficient production hosts. The use of systems-level studies makes it possible to comprehensively understand the impact of pathway engineering within the context of the entire host metabolism, to diagnose stresses due to product synthesis, and provides the rationale to cost-effectively engineer optimal industrial microorganisms.

  2. Bioelectrochemical reduction of volatile fatty acids in anaerobic digestion effluent for the production of biofuels.

    Science.gov (United States)

    Kondaveeti, Sanath; Min, Booki

    2015-12-15

    This study proves for the first time the feasibility of biofuel production from anaerobic digestion effluent via bioelectrochemical cell operation at various applied cell voltages (1.0, 1.5 and 2.0 V). An increase in cell voltage from 1 to 2 V resulted in more reduction current generation (-0.48 to -0.78 mA) at a lowered cathode potential (-0.45 to -0.84 mV vs Ag/AgCl). Various alcohols were produced depending on applied cell voltages, and the main products were butanol, ethanol, and propanol. Hydrogen and methane production were also observed in the headspace of the cell. A large amount of lactic acid was unexpectedly formed at all conditions, which might be the primary cause of the limited biofuel production. The addition of neutral red (NR) to the system could increase the cathodic reduction current, and thus more biofuels were produced with an enhanced alcohol formation compared to without a mediator.

  3. Monster potential meets potential monster: pros and cons of deploying genetically modified microalgae for biofuels production.

    Science.gov (United States)

    Flynn, K J; Mitra, A; Greenwell, H C; Sui, J

    2013-02-01

    Biofuels production from microalgae attracts much attention but remains an unproven technology. We explore routes to enhance production through modifications to a range of generic microalgal physiological characteristics. Our analysis shows that biofuels production may be enhanced ca fivefold through genetic modification (GM) of factors affecting growth rate, respiration, photoacclimation, photosynthesis efficiency and the minimum cell quotas for nitrogen and phosphorous (N : C and P : C). However, simulations indicate that the ideal GM microalgae for commercial deployment could, on escape to the environment, become a harmful algal bloom species par excellence, with attendant risks to ecosystems and livelihoods. In large measure, this is because an organism able to produce carbohydrate and/or lipid at high rates, providing stock metabolites for biofuels production, will also be able to attain a stoichiometric composition that will be far from optimal as food for the support of zooplankton growth. This composition could suppress or even halt the grazing activity that would otherwise control the microalgal growth in nature. In consequence, we recommend that the genetic manipulation of microalgae, with inherent consequences on a scale comparable to geoengineering, should be considered under strict international regulation. PMID:24427510

  4. Engineering microbes for tolerance to next-generation biofuels

    Directory of Open Access Journals (Sweden)

    Dunlop Mary J

    2011-09-01

    Full Text Available Abstract A major challenge when using microorganisms to produce bulk chemicals such as biofuels is that the production targets are often toxic to cells. Many biofuels are known to reduce cell viability through damage to the cell membrane and interference with essential physiological processes. Therefore, cells must trade off biofuel production and survival, reducing potential yields. Recently, there have been several efforts towards engineering strains for biofuel tolerance. Promising methods include engineering biofuel export systems, heat shock proteins, membrane modifications, more general stress responses, and approaches that integrate multiple tolerance strategies. In addition, in situ recovery methods and media supplements can help to ease the burden of end-product toxicity and may be used in combination with genetic approaches. Recent advances in systems and synthetic biology provide a framework for tolerance engineering. This review highlights recent targeted approaches towards improving microbial tolerance to next-generation biofuels with a particular emphasis on strategies that will improve production.

  5. Natural gas and biofuel as feedstock for hydrogen production on Ni catalysts

    Institute of Scientific and Technical Information of China (English)

    Pasquale Corbo; Fortunato Migliardini

    2009-01-01

    In this article,the aptitude of natural gas as feedstock in steam reforming process for hydrogen production is compared with that of different liquid fuels (pure compounds and commercial fuels),with the aim to investigate the potentialities of biofuels to overcome the CO2 emission problems deriving from fossil fuel processing.The performances of a nickel based catalyst (commercially used in steam reforming of natural gas) were evaluated in terms of feed conversion and yield to the different products as function of temperature,space velocity and water/fuel ratio.Furthermore,a preliminary evaluation of catalyst durability was effected by monitoring yield to H2 versus time on stream and measuring coke formation at the end of experimental tests.High yields to hydrogen were obtained with ail fuels investigated,whereas the deactivation phenomena,which are correlated to carbon deposition on the catalyst,were observed with all tested fuels,except for methane and biofuel.

  6. Lipid Recovery from Wet Oleaginous Microbial Biomass for Biofuel Production: A Critical Review

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Tao; Knoshaug, Eric P.; Pienkos, Philip T.; Laurens, Lieve M. L.

    2016-09-01

    Biological lipids derived from oleaginous microorganisms are promising precursors for renewable biofuel productions. Direct lipid extraction from wet cell-biomass is favored because it eliminates the need for costly dehydration. However, the development of a practical and scalable process for extracting lipids from wet cell-biomass is far from ready to be commercialized, instead, requiring intensive research and development to understand the lipid accessibility, mechanisms in mass transfer and establish robust lipid extraction approaches that are practical for industrial applications. This paper aims to present a critical review on lipid recovery in the context of biofuel productions with special attention to cell disruption and lipid mass transfer to support extraction from wet biomass.

  7. Fatty aldehydes in cyanobacteria are a metabolically flexible precursor for a diversity of biofuel products.

    Directory of Open Access Journals (Sweden)

    Brett K Kaiser

    Full Text Available We describe how pathway engineering can be used to convert a single intermediate derived from lipid biosynthesis, fatty aldehydes, into a variety of biofuel precursors including alkanes, free fatty acids and wax esters. In cyanobacteria, long-chain acyl-ACPs can be reduced to fatty aldehydes, and then decarbonylated to alkanes. We discovered a cyanobacteria class-3 aldehyde-dehydrogenase, AldE, that was necessary and sufficient to instead oxidize fatty aldehyde precursors into fatty acids. Overexpression of enzymes in this pathway resulted in production of 50 to 100 fold more fatty acids than alkanes, and the fatty acids were secreted from the cell. Co-expression of acyl-ACP reductase, an alcohol-dehydrogenase and a wax-ester-synthase resulted in a third fate for fatty aldehydes: conversion to wax esters, which accumulated as intracellular lipid bodies. Conversion of acyl-ACP to fatty acids using endogenous cyanobacterial enzymes may allow biofuel production without transgenesis.

  8. A Novel Aeration Method for the Preparation of Algae (Dunaliella Salina Biomass for Biofuel Production.

    Directory of Open Access Journals (Sweden)

    U.O. Enwereuzoh

    2014-09-01

    Full Text Available Preparation of algae (Dunaliela Salina biomass in ammonia (NH4 + and nitrate (NO3 - growth media for biofuel production was investigated, with special attention on the elimination of inhibitory oxygen that adversely affects algae growth. A novel aeration method based on high and efficient transfer of carbon dioxide (CO2 required to stabilize the CO2 of the algae growth medium in a short time was adopted for the elimination of the inhibitory oxygen. The novel aeration method was found to increase the algae growth rate in the growth media investigated as suggested by increases in pH and decreases in dissolved oxygen concentration. However, algae grown in ammonia medium showed 17% higher growth rate than algae grown in nitrate medium. The high mass transfer of CO2 and high energy efficiency make the novel aeration method of algae growth in ammonia medium better suited for high yield of algae biomass for biofuel production.

  9. Natural Resources and Local Development: The Argentinian Oilseed Complex and Biofuel Production

    Directory of Open Access Journals (Sweden)

    Eliana Scialabba

    2014-01-01

    Full Text Available Argentina is very rich in natural resources, particularly those linked to the oilseed production. Global demand and improved means of production, have resulted in a mature and developed production infrastructure geared toward export. This generates not just revenue, but also has other positive effects, such as the creation of jobs and sustainable development. In this context, biofuel production adds multidimensional value to the vegetable oil industry and generations many spin-off industries.

  10. Energy and economic potential of maize straw used for biofuels production

    Directory of Open Access Journals (Sweden)

    Zbytek Zbyszek

    2016-01-01

    Full Text Available The paper presents the energy and economic comparison of two technologies of maize straw utilization: solid biofuel production (briquettes and methane fermentation. The research experiments have shown that maize straw is the material which can be efficiently implemented in both technologies. Maize straw usage as briquettes can generate more energy (10.956 GJ Mg−1 than methane fermentation (9.74. In Europe, biogas is used in co-generation units for production of electric and heat energy. Due to higher price of electricity, economic profitability of maize straw usage for biogas production is over twice higher (182 USD than in case of briquettes production (96 USD.

  11. Space for innovation for sustainable community-based biofuel production and use: Lessons learned for policy from Nhambita community, Mozambique

    Energy Technology Data Exchange (ETDEWEB)

    Schut, Marc, E-mail: marc.schut@wur.nl [Communication and Innovation Studies, Wageningen University and Research Centre, P.O. Box 8130, 6700 EW Wageningen (Netherlands); Paassen, Annemarie van, E-mail: annemarie.vanpaassen@wur.nl [Communication and Innovation Studies, Wageningen University and Research Centre, P.O. Box 8130, 6700 EW Wageningen (Netherlands); Leeuwis, Cees, E-mail: cees.leeuwis@wur.nl [Communication and Innovation Studies, Wageningen University and Research Centre, P.O. Box 8130, 6700 EW Wageningen (Netherlands); Bos, Sandra, E-mail: sandra-bos@live.nl [Social Engineer and SME-specialist, FACT Foundation, Eindhoven (Netherlands); Leonardo, Wilson, E-mail: wilson.leonardo@wur.nl [Plant Production Systems, Wageningen University and Research Centre, Wageningen (Netherlands); Lerner, Anna, E-mail: klerner@worldbank.org [Climate Change Mitigation Team, Global Environment Facility, World Bank, Washington, DC (United States)

    2011-09-15

    This paper provides insights and recommendations for policy on the opportunities and constrains that influence the space for innovation for sustainable community-based biofuel production and use. Promoted by the Mozambican government, Nhambita community established jatropha trials in 2005. Initial results were promising, but crop failure and the absence of organized markets led to scepticism amongst farmers. We start from the idea that the promotion of community-based biofuel production and use requires taking interactions between social-cultural, biophysical, economic, political and legal subsystems across different scales and levels of analysis through time into account. Our analysis demonstrates that heterogeneous farming strategies and their synergies at community level should be carefully assessed. Furthermore, national and international political and legal developments, such as the development of biofuel sustainability criteria, influence the local space in which community-based biofuel developments take place. We conclude that ex-ante integrated assessment and creating an enabling environment can enhance space for sustainable community-based biofuel production and use. It may provide insights into the opportunities and constraints for different types of smallholders, and promote the development of adequate policy mechanisms to prevent biofuels from becoming a threat rather than an opportunity for smallholders. - Highlights: > This paper explores space for innovation for community-based biofuel production and use. > Heterogeneous farming strategies and their synergies at community level are key. > Farmers have little trust in jatropha due to crop failure and absence of markets. > (Inter)national biofuel policies influence space for local biofuel production and use. > Policies should focus on ex-ante integrated assessment and creating an enabling environment.

  12. Space for innovation for sustainable community-based biofuel production and use: Lessons learned for policy from Nhambita community, Mozambique

    International Nuclear Information System (INIS)

    This paper provides insights and recommendations for policy on the opportunities and constrains that influence the space for innovation for sustainable community-based biofuel production and use. Promoted by the Mozambican government, Nhambita community established jatropha trials in 2005. Initial results were promising, but crop failure and the absence of organized markets led to scepticism amongst farmers. We start from the idea that the promotion of community-based biofuel production and use requires taking interactions between social-cultural, biophysical, economic, political and legal subsystems across different scales and levels of analysis through time into account. Our analysis demonstrates that heterogeneous farming strategies and their synergies at community level should be carefully assessed. Furthermore, national and international political and legal developments, such as the development of biofuel sustainability criteria, influence the local space in which community-based biofuel developments take place. We conclude that ex-ante integrated assessment and creating an enabling environment can enhance space for sustainable community-based biofuel production and use. It may provide insights into the opportunities and constraints for different types of smallholders, and promote the development of adequate policy mechanisms to prevent biofuels from becoming a threat rather than an opportunity for smallholders. - Highlights: → This paper explores space for innovation for community-based biofuel production and use. → Heterogeneous farming strategies and their synergies at community level are key. → Farmers have little trust in jatropha due to crop failure and absence of markets. → (Inter)national biofuel policies influence space for local biofuel production and use. → Policies should focus on ex-ante integrated assessment and creating an enabling environment.

  13. A Novel Aeration Method for the Preparation of Algae (Dunaliella Salina) Biomass for Biofuel Production.

    OpenAIRE

    U.O. Enwereuzoh; G.N. Onyeagoro

    2014-01-01

    Preparation of algae (Dunaliela Salina) biomass in ammonia (NH4 + ) and nitrate (NO3 - ) growth media for biofuel production was investigated, with special attention on the elimination of inhibitory oxygen that adversely affects algae growth. A novel aeration method based on high and efficient transfer of carbon dioxide (CO2) required to stabilize the CO2 of the algae growth medium in a short time was adopted for the elimination of the inhibitory oxygen. The novel aeration method was found...

  14. Applications and perspectives of multi-parameter flow cytometry to microbial biofuels production processes 

    OpenAIRE

    Silva, Teresa Lopes da; Roseiro, J. Carlos; Reis, Alberto

    2012-01-01

    Conventional microbiology methods used to monitor microbial biofuels production are based on off-line analyses. The analyses are, unfortunately, insufficient for bioprocess optimization. Real time process control strategies, such as flow cytometry (FC), can be used to monitor bioprocess development (at-line) by providing single cell information that improves process model formulation and validation. This paper reviews the current uses and potential applications of FC in biodiesel, bioethanol,...

  15. Greenhouse gas emissions of biofuels: improving Life Cycle Assessments by taking into account local production factors

    OpenAIRE

    Bessou, Cécile

    2009-01-01

    Life Cycle Assessments (LCA) make it possible to check for all polluting emissions and potential environmental impacts of biofuels throughout the product chain. However, LCA emission factors only give an estimate of average potential emissions, notably not considering the local kinetics of N2O emissions, the most important agricultural greenhouse gas. Our objective was first to analyse how soil compaction affects N2O emissions by combining field experiments and modelling. With automatic chamb...

  16. Cost structures and life cycle impacts of algal biomass and biofuel production

    Science.gov (United States)

    Christiansen, Katrina Lea

    2011-12-01

    Development and extraction of energy sources, energy production and energy use have huge economic, environmental and geopolitical impacts. Increasing energy demands in tandem with reductions in fossil fuel production has led to significant investments in research into alternative forms of energy. One that is promising but yet not commercially established is the production of biofuel from algae. This research quantitatively assessed the potential of algae biofuel production by examining its cost and environmental impacts. First, two models developed by the RAND corporation were employed to assess Cost Growth defined as the ratio of actual costs to estimated costs, and Plant Performance defined as the ratio of actual production levels to design performance, of three algal biofuel production technologies. The three algal biofuel production technologies examined to open raceway ponds (ORPs), photobioreactors (PBRs), and a system that couples PBRs to ORPs (PBR-ORPs). Though these analyses lack precision due to uncertainty, the results highlight the risks associated with implementing algal biofuel systems, as all scenarios examined were predicted to have Cost Growth, ranging from 1.2 to 1.8, and Plant Performance was projected as less than 50% of design performance for all cases. Second, the Framework the Evaluation of Biomass Energy Feedstocks (FEBEF) was used to assess the cost and environmental impacts of biodiesel produced from three algal production technologies. When these results were compared with ethanol from corn and biodiesel from soybeans, biodiesel from algae produced from the different technologies were estimated to be more expensive, suffered from low energy gains, and did not result in lower greenhouse gas emissions. To identify likely routes to making algal biofuels more competitive, a third study was undertaken. In this case, FEBEF was employed to examine pinch-points (defined as the most costly, energy consuming, greenhouse gas producing processes), in

  17. Biofuel production from catalytic cracking of woody oils.

    Science.gov (United States)

    Xu, Junming; Jiang, Jianchun; Chen, Jie; Sun, Yunjuan

    2010-07-01

    The catalytic cracking reactions of several kinds of woody oils have been studied. The products were analyzed by GC-MS and FTIR and show the formation of olefins, paraffins and carboxylic acids. Several kinds of catalysts were compared. It was found that the fraction distribution of product was modified by using base catalysts such as CaO. The products from woody oils showed good cold flow properties compared with diesel used in China. The results presented in this work have shown that the catalytic cracking of woody oils generates fuels that have physical and chemical properties comparable to those specified for petroleum based fuels. PMID:20206508

  18. Production of biofuel using molluscan pseudofeces derived from algal cells

    Science.gov (United States)

    Das, Keshav C.; Chinnasamy, Senthil; Shelton, James; Wilde, Susan B.; Haynie, Rebecca S.; Herrin, James A.

    2012-08-28

    Embodiments of the present disclosure provide for novel strategies to harvest algal lipids using mollusks which after feeding algae from the growth medium can convert algal lipids into their biomass or excrete lipids in their pseudofeces which makes algae harvesting energy efficient and cost effective. The bioconverter, filter-feeding mollusks and their pseudofeces can be harvested and converted to biocrude using an advanced thermochemical liquefaction technology. Methods, systems, and materials are disclosed for the harvest and isolation of algal lipids from the mollusks, molluscan feces and molluscan pseudofeces.

  19. Energy production from burning of mixtures of source-sorted waste fractions and biofuels; Sameldning av biobraenslen med kaellsorterade avfallsfraktioner

    Energy Technology Data Exchange (ETDEWEB)

    Olsson, Rolf; Marklund, Stellan; Nilsson, Calle; Burvall, Jan; Hedman, Bjoern [Swedish Univ. of Agricultural Sciences, Umeaa (Sweden). Dept. of Agricultural Research for Northern Sweden

    2001-02-01

    Energy production through co-combustion of biofuel and the dry fraction of household waste might be an alternative in the future for rural district areas since the present system for recovery has been criticized. So far the results from the co-combustion of briquettes made by energy grass, Reed Canary grass, in mixture with very well separated dry fraction of household waste shows that new developed commercial boiler technology, 100-1000 kW, have been well designed for good function, high efficiency and low emissions using ordinary cyclone flue gas cleaning. By consideration of the new EU incineration waste directive for co-combustion of biofuel and waste fractions the achieved emission levels from this trials in many cases seem to fulfill the directive including critical parameters such as CO and dioxins without highly advanced flue gas cleaning. Mixture of waste together with energy grass does not raise the emission levels of HCl compared to pure energy grass combustion. The importance of developing systems for well sorted dry fractions of household waste have been verified in the project. The quality insurance of the fuel is necessary to consider the new EU-directive. In this project studies of systems for sorting of dry waste have been compared. A system in which the sorting part can be identified and attained by information and premium awards shows obvious advantages compared with local central placed container systems for the dry waste.

  20. Clean solid biofuel production from high moisture content waste biomass employing hydrothermal treatment

    International Nuclear Information System (INIS)

    Highlights: • Clean solid biofuel was produced from wet waste biomass by hydrothermal treatment. • Waste biomass resources and fuel production processes were discussed. • The upgrading, dechlorination, denitrification, and coalification was illustrated. • Fuel combustion performances, energy and mass balances and economics were reviewed. • The newest results and perspectives for further developments were discussed. - Abstract: Our society currently faces three challenges, including resource depletion, waste accumulation and environmental degradation, leading to rapidly escalating raw material costs and increasingly expensive and restrictive waste disposal legislation. This work aims to produce clean solid biofuel from high moisture content waste biomass (bio-waste) with high nitrogen (N)/chlorine (Cl) content by mild hydrothermal (HT) conversion processes. The newest results are summarized and discussed in terms of the mechanical dewatering and upgrading, dechlorination, denitrification and coalification resulting from the HT pretreatment. Moreover, both the mono-combustion and co-combustion characteristics of the solid fuel are reviewed by concentrating on the pollutants emission control, especially the NO emission properties. In addition, the feasibility of this HT solid biofuel production process is also discussed in terms of “Energy Balance and economic viability”. As an alternative to dry combustion/dry pyrolysis/co-combustion, the HT process, combining the dehydration and decarboxylation of a biomass to raise its carbon content aiming to achieve a higher calorific value, opens up the field of potential feedstock for lignite-like solid biofuel production from a wide range of nontraditional renewable and plentiful wet agricultural residues, sludge and municipal wastes. It would contribute to a wider application of HT pretreatment bio-wastes for safe disposal and energy recycling

  1. The effect of cellulosic biofuel production on water resources at a regional scale

    Science.gov (United States)

    Christopher, S. F.; Scheonholtz, S. H.; Nettles, J. E.

    2012-12-01

    The U.S. government has mandated production of 36 billion gallons of renewable fuels by 2022, of which 16 billion gallons are required to be cellulosic biofuels. Production of cellulosic biomass offers a promising alternative to corn-based systems because large-scale production of corn-based ethanol often requires irrigation and is associated with increased erosion, excess sediment export, and enhanced leaching of nitrogen and phosphorus. Although cultivation of switchgrass using standard agricultural practices is one option being considered for production of cellulosic biomass, intercropping cellulosic biofuel crops within managed forests could provide feedstock without primary land use change or the water resources impacts associated with annual crops. There are data sets and models that have been used to evaluate effects of agriculturally-based biofuel options on water quantity and quality, but the evaluation - from instrumentation through data analysis - is designed for these more disturbed systems and is not appropriate for the more subtle changes anticipated from a pine/switchgrass systems. Currently, there is no known hydrologic model that can explicitly assess the effect of intercropping on water resources. However, these models can evaluate the effects of growing switchgrass on water resources and would be useful in identifying the "worst case scenario". We used the Soil Water Assessment Tool (SWAT), a physically-based hydrologic model, to examine effects of large scale conversion of pine plantations to switchgrass biofuel production on water resources in the ~ 5 mil ha Tombigbee Watershed in the southeastern U.S. Publically available datasets were used as input to the model and for calibration. To improve calibration statistics, five tree age classes were added to the model to more appropriately represent existing forested systems in the region, which are not included within the standard model set-up. Results suggest land use conversions result in 4 and

  2. Case studies of large-scale biorefining concepts for production of biofuels, fertilizer, and feed - recent Danish developments

    Energy Technology Data Exchange (ETDEWEB)

    Holm-Nielsen, Jens Bo; Boland, Ludovic; Honnay, Stephanie; Cybulska, Iwona; Brudecki, Grzegorz (Aalborg Univ., Esbjerg Inst. of Technology, Bioenergy and Biotechnology Research Group, DK-6700 Esbjerg (Denmark)). e-mail: jhn@bio.sdu.dk; Madsen, Michael (Aalborg Univ., Esbjerg Inst. of Technology, ACABS Research Group, DK-6700 Esbjerg (Denmark))

    2008-10-15

    sustainable way. We are looking further than merely discussing generations of technology. Two biorefinery concepts have been developed, R and D evaluated before construction. They differ with respect to the feedstocks they utilize; maize silage (corn-silage) and whole sugar beets crops (including leafs and stems). Case 1: Toender Biofuel initiative is a biorefinery project in the southern part of Denmark. Although still being in the planning phase, the project integrates many state-of-the-art solutions as well as innovative technology. The biorefinery concept integrates production of bioethanol for transportation purposes, biogas generation for on-site combined heat and power production, and manufacturing of animal feed based on protein-rich residual from the fermentation processes. Future add-ons include a facility for biodiesel production or higher value chain products in the second project phase. Case 2: Djursland Biofuel initiative involves farmers in further processing, instead of just sealing feedstocks to biorefineries, the crop is being fermented at the farm site or in small groups of farms. After fermentation, the broth is separate by means of low technology equipment at the farm before being transported to centralized processing plants in tankers. The final energy demanding distillation is part carried out centrally. By increasing R and D for ethanol-water separation at mobile units or by innovation technology the first distilling steps should be handled at the farms. This is truly integration of the farmers in the entire value chain allowing them to take part in higher profit rate of the value chain. Various pre-treatment steps are under consideration including wet milling, thermal hydrolysis among others. Advanced Process Analytical Technologies also have to be integrated into the biorefineries in order to ensure optimal process understanding and, in time, increased yield

  3. Corrosion in systems for storage and transportation of petroleum products and biofuels identification, monitoring and solutions

    CERN Document Server

    Groysman, Alec

    2014-01-01

    This book treats corrosion as it occurs and affects processes in real-world situations, and thus points the way to practical solutions. Topics described include the conditions in which petroleum products are corrosive to metals; corrosion mechanisms of petroleum products; which parts of storage tanks containing crude oils and petroleum products undergo corrosion; dependence of corrosion in tanks on type of petroleum products; aggressiveness of petroleum products to polymeric material; how microorganisms take part in corrosion of tanks and pipes containing petroleum products; which corrosion monitoring methods are used in systems for storage and transportation of petroleum products; what corrosion control measures should be chosen; how to choose coatings for inner and outer surfaces of tanks containing petroleum products; and how different additives (oxygenates, aromatic solvents) to petroleum products and biofuels influence metallic and polymeric materials. The book is of interest to corrosion engineers, mat...

  4. Lactobacillus casei as a biocatalyst for biofuel production.

    Science.gov (United States)

    Vinay-Lara, Elena; Wang, Song; Bai, Lina; Phrommao, Ekkarat; Broadbent, Jeff R; Steele, James L

    2016-09-01

    Microbial fermentation of sugars from plant biomass to alcohols represents an alternative to petroleum-based fuels. The optimal biocatalyst for such fermentations needs to overcome hurdles such as high concentrations of alcohols and toxic compounds. Lactic acid bacteria, especially lactobacilli, have high innate alcohol tolerance and are remarkably adaptive to harsh environments. This study assessed the potential of five Lactobacillus casei strains as biocatalysts for alcohol production. L. casei 12A was selected based upon its innate alcohol tolerance, high transformation efficiency and ability to utilize plant-derived carbohydrates. A 12A derivative engineered to produce ethanol (L. casei E1) was compared to two other bacterial biocatalysts. Maximal growth rate, maximal optical density and ethanol production were determined under conditions similar to those present during alcohol production from lignocellulosic feedstocks. L. casei E1 exhibited higher innate alcohol tolerance, better growth in the presence of corn stover hydrolysate stressors, and resulted in higher ethanol yields. PMID:27312380

  5. Sustainable Process Design of Biofuels: Bioethanol Production from Cassava rhizome

    DEFF Research Database (Denmark)

    Mangnimit, S.; Malakul, P.; Gani, Rafiqul

    2013-01-01

    This study is focused on the sustainable process design of bioethanol production from cassava rhizome. The study includes: process simulation, sustainability analysis, economic evaluation and life cycle assessment (LCA). A steady state process simulation if performed to generate a base case design...... of the bioethanol conversion process using cassava rhizome as a feedstock. The sustainability analysis is performed to analyze the relevant indicators in sustainability metrics, to definedesign/retrofit targets for process improvements. Economic analysis is performed to evaluate the profitability of the process...... in order to identify the most sustainable design for the production of ethanol. The capacity for ethanol production from cassava rhizome is set to 150,000 liters/day, which is about 1.3 % of the total demand of ethanol in Thailand. LCA on the base case design pointed to large amounts of CO2 and CO...

  6. Production of biofuels from pretreated microalgae biomass by anaerobic fermentation with immobilized Clostridium acetobutylicum cells.

    Science.gov (United States)

    Efremenko, E N; Nikolskaya, A B; Lyagin, I V; Senko, O V; Makhlis, T A; Stepanov, N A; Maslova, O V; Mamedova, F; Varfolomeev, S D

    2012-06-01

    The purpose of this work was to study the possible use of pretreated biomass of various microalgae and cyanobacteria as substrates for acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum cells immobilized into poly(vinyl alcohol) cryogel. To this end, the biochemical composition of photosynthetic microorganisms cultivated under various conditions was studied. The most efficient technique for pretreating microalgal biomass for its subsequent conversion into biofuels appeared to be thermal decomposition at 108 °C. For the first time the maximum productivity of the ABE fermentation in terms of hydrogen (8.5 mmol/L medium/day) was obtained using pretreated biomass of Nannochloropsis sp. Maximum yields of butanol and ethanol were observed with Arthrospira platensis biomass used as the substrate. Immobilized Clostridium cells were demonstrated to be suitable for multiple reuses (for a minimum of five cycles) in ABE fermentation for producing biofuels from pretreated microalgal biomass.

  7. Spatial analysis of the potential crops for the production of biofuels in Argentina

    Energy Technology Data Exchange (ETDEWEB)

    Carballo, Stella; Marco, Noelia Flores; Anschau, Alicia [Centro de Investigaciones de Recursos Naturales (CIRN/INTA), Buenos Aires (Argentina). Inst. de Tecnologia Agropecuaria. Inst. de Clima y Agua], E-mail: scarballo@cnia.inta.gov.ar; Hilbert, Jorge [Instiuto de Ingenieria Rural (CIA/INTA), Buenos Aires (Argentina)], E-mail: hilbert@cnia.inta.gov.ar

    2008-07-01

    The increase in biofuels production has been rising in the last ten years at a high rate. Argentina as one of the main crop producers in the world has a great potential to contribute with high volumes of biofuels. At present time common crops are used for large scale production but new alternatives are under study in different regions of the country. The increase in pressure for expansion also raises concerns on the impact on ecology issues such as soil erosion and biodiversity. Looking at a national level INTA has been working on the construction of a GIS were different crops were placed. The purpose is to identify critical information, to raise a methodology to obtain accurate and up-to date thematic maps using satellite images, to feed a GIS and to integrate the different layers to estimate biomass potentials for energy supply in our country, assessing potential land availability for biofuel crops or plantations to be made with ecological, economic and social sustainability bases. (author)

  8. Bioprospecting for hyper-lipid producing microalgal strains for sustainable biofuel production.

    Science.gov (United States)

    Mutanda, T; Ramesh, D; Karthikeyan, S; Kumari, S; Anandraj, A; Bux, F

    2011-01-01

    Global petroleum reserves are shrinking at a fast pace, increasing the demand for alternate fuels. Microalgae have the ability to grow rapidly, and synthesize and accumulate large amounts (approximately 20-50% of dry weight) of neutral lipid stored in cytosolic lipid bodies. A successful and economically viable algae based biofuel industry mainly depends on the selection of appropriate algal strains. The main focus of bioprospecting for microalgae is to identify unique high lipid producing microalgae from different habitats. Indigenous species of microalgae with high lipid yields are especially valuable in the biofuel industry. Isolation, purification and identification of natural microalgal assemblages using conventional techniques is generally time consuming. However, the recent use of micromanipulation as a rapid isolating tool allows for a higher screening throughput. The appropriate media and growth conditions are also important for successful microalgal proliferation. Environmental parameters recorded at the sampling site are necessary to optimize in vitro growth. Identification of species generally requires a combination of morphological and genetic characterization. The selected microalgal strains are grown in upscale systems such as raceway ponds or photobireactors for biomass and lipid production. This paper reviews the recent methodologies adopted for site selection, sampling, strain selection and identification, optimization of cultural conditions for superior lipid yield for biofuel production. Energy generation routes of microalgal lipids and biomass are discussed in detail. PMID:20624676

  9. Trends and sustainability criteria of the production and use of liquid biofuels

    International Nuclear Information System (INIS)

    Environmental impacts associated with the use of fossil fuels, rising prices, potential limitations in supply and concerns about regional and national security are driving the development and use of biomass for bioenergy, biofuels and bioproducts. However, the use of biomass does not automatically imply that its production, conversion and use are sustainable. Conflicts between various ecosystem services (economic production of food, fodder and fuels, biodiversity, social and cultural values, etc.) that are provided by fertile land are increasing as well. Hence, a developed thinking on how to balance between these services is desirable. There is a significant amount of information available on biofuels and their sustainability. In this paper, different initiatives and sustainability criteria for biofuels are presented and assessed. 35 criteria were found in emerging sustainability assessment frameworks. The majority of 12 criteria were focused on environmental issues, 4 were social and only 1 was economic. Energy balance and greenhouse gas balance were perceived as especially critical, social criteria ranked generally low. Although being perceived as important, food security ranked very low. (author)

  10. Partnering with Industry to Advance Biofuels, NREL's Integrated Biorefinery Research Facility (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    2010-10-01

    Fact sheet describing NREL's Integrated Biorefinery Research Facility and its availability to biofuels' industry partners who want to operate, test, and develop biorefining technology and equipment.

  11. Biofuel production from acid-impregnated willow and switchgrass

    NARCIS (Netherlands)

    Bakker, R.R.; Gosselink, R.J.A.; Maas, R.H.W.; Vrije, de T.; Jong, de E.; Groenestijn, van J.W.; Hazewinkel, J.H.O.

    2004-01-01

    As part of a broader technical and economic feasibility study, we studied production of bioethanol from two types of lignocellulosic biomass by way of concentrated acid impregnation at low temperature. Willow chips and switchgrass were submitted to various impregnation techniques with concentrated s

  12. Fuel production from biomass: generation of liquid biofuels

    Directory of Open Access Journals (Sweden)

    Carmen Ghergheleş

    2008-05-01

    Full Text Available Anaerobic fermentation processes mayalso be used to produce liquid fuels frombiological raw materials. An example is theethanol production from glucose, known asstandard yeast fermentation in the beer, wine andliquor industries. It has to take place in steps, suchthat the ethanol is removed (by distillation ordehydrator application whenever itsconcentration approaches a value (around 12%which would impede reproduction of the yeastculture.

  13. Processing of Brassica seeds for feedstock in biofuels production

    Science.gov (United States)

    Several Brassica species are currently being evaluated to develop regionalized production systems based on their suitability to the environment and with the prevailing practices of growing commodity food crops like wheat, corn, and soybeans. This integrated approach to farming will provide high qual...

  14. Energy production study of crops with biofuel potential in Argentina

    Energy Technology Data Exchange (ETDEWEB)

    Donato, Lidia; Huerga, Ignacio; Hilbert, Jorge [Instituto Nacional de Tecnologia Agropecuaria (CIA/INTA), Buenos Aires (Argentina). Centro de Investigacion de Agroindustria. Inst. de Ingenieria Rural], Emails: ingdonato@cnia.inta.gov.ar, ihuerga@cnia.inta.gov.ar, hilbert@cnia.inta.gov.ar

    2008-07-01

    The present study is focus on the final energy balance of bioenergy production in Argentina using soybean, sunflower, rapeseed, corn and sorghum as feedstocks. The balance considers the difference between the energy contained per unit and the amount used for its generation in all the different steps from sowing to final destination. For direct energy consumption Costo Maq software was employed using local fuel consumption forecast for each field labor. Particular attention is paid to the energy consumption in the agricultural steps considering the distinctive no till system spread out in Argentina that has a very low energy input. Direct and indirect energy were considered in the different steps of bioethanol and biodiesel generation. Industrial conversion consumption was based on international literature data. Comparisons were made between tilled and no till practices and considering or not the energy contained in co products. Results indicate a balance ranging from 0.96 to 1.54 not considering the co products. If co products were introduced the balances ranged between 1.09 and 4.67. (author)

  15. Consequences of agro-biofuel production for greenhouse gas emissions

    DEFF Research Database (Denmark)

    Carter, Mette Sustmann; Johansen, Anders; Hauggard-Nielsen, Henrik;

    2008-01-01

    The objective of the study was to examine the effect on N2O and CH4 emissions when residues from bio-energy production are recycling as organic fertilizer for a maize energy crop. The study showed that the N2O emission associated with the cultivation of the maize crop offset a considerable faction...

  16. Microalgae for the production of bulk chemicals and biofuels

    NARCIS (Netherlands)

    Wijffels, R.H.; Barbosa, M.J.; Eppink, M.H.M.

    2010-01-01

    The feasibility of microalgae production for biodiesel was discussed. Although algae are not yet produced at large scale for bulk applications, there are opportunities to develop this process in a sustainable way. It remains unlikely, however, that the process will be developed for biodiesel as the

  17. Soil carbon sequestration and land use change associated with biofuel production: Empirical evidence

    Energy Technology Data Exchange (ETDEWEB)

    Qin, Zhangcai; Dunn, Jennifer B.; Kwon, Hoyoung; Mueller, Steffen; Wander, Michelle M.

    2016-01-01

    Soil organic carbon (SOC) change can be a major impact of land use change (LUC) associated with biofuel feedstock production. By collecting and analyzing data from worldwide field observations with major LUCs from cropland, grassland and forest to lands producing biofuel crops (i.e., corn, switchgrass, Miscanthus, poplar and willow), we were able to estimate SOC response ratios and sequestration rates and evaluate the effects of soil depth and time scale on SOC change. Both the amount and rate of SOC change were highly dependent on the specific land transition. Irrespective of soil depth or time horizon, cropland conversions resulted in an overall SOC gain of 6-14% relative to initial SOC level, while conversion from grassland or forest to corn (without residue removal) or poplar caused significant carbon loss (9-35%). No significant SOC changes were observed in land converted from grasslands or forests to switchgrass, Miscanthus or willow. The SOC response ratios were similar in both 0-30 and 0-100 cm soil depths in most cases, suggesting SOC changes in deep soil and that use of top soil only for SOC accounting in biofuel life cycle analysis (LCA) might underestimate total SOC changes. Soil carbon sequestration rates varied greatly among studies and land transition types. Generally, the rates of SOC change tended to be the greatest during the 10 years following land conversion, and had declined to approach 0 within about 20 years for most LUCs. Observed trends in SOC change were generally consistent with previous reports. Soil depth and duration of study significantly influence SOC change rates and so should be considered in carbon emission accounting in biofuel LCA. High uncertainty remains for many perennial systems, field trials and modeling efforts are needed to determine the site- and system-specific rates and direction of change associated with their production.

  18. Influence of By-Products Obtained from Biofuels Industry on Productive Performances of Lambs

    Directory of Open Access Journals (Sweden)

    Olimpia Colibar

    2010-05-01

    Full Text Available Experiments were performed on 100 lambs, of common race, divided into 4 homogeneous groups of 25 lambs each, which were fed, differentiated for 3 weeks. Lots 1.2 and 3 received a supplement consisting of by-products obtained from the manufacture of biofuels in the area: Feed rations had similar nutritive values for all lambs in the experimental plots. The amount of crude protein in concentrates ratio was 17.40% for group 1, 18.44% for group 2, 17.60% for group 3, and 17.00% for group 4. Bulky feed were given ad libitum for all groups. After the first week of the experiment there was a spectacular evolution of body weight gain. All experimental lots were situated beyond the control group. Body mass growth rate is 3.5 times higher in the group fed with supplement of sunflower meal, 4.16 times higher in that fed with soybean meal supplements and 5.2 times higher in the group fed with additional rape meal. After the second week, the differences are decreasing, as the absolute value of body weight gain.

  19. Evaluation of filamentous green algae as feedstocks for biofuel production.

    Science.gov (United States)

    Zhang, Wei; Zhao, Yonggang; Cui, Binjie; Wang, Hui; Liu, Tianzhong

    2016-11-01

    Compared with unicellular microalgae, filamentous algae have high resistance to grazer-predation and low-cost recovery in large-scale production. Green algae, as the most diverse group of algae, included numerous filamentous genera and species. In this study, records of filamentous genera and species in green algae were firstly censused and classified. Then, seven filamentous strains subordinated in different genera were cultivated in bubbled-column to investigate their growth rate and energy molecular (lipid and starch) capacity. Four strains including Stigeoclonium sp., Oedogonium nodulosum, Hormidium sp. and Zygnema extenue were screened out due to their robust growth. And they all could accumulate triacylglycerols and starch in their biomass, but with different capacity. After nitrogen starvation, Hormidium sp. and Oedogonium nodulosum respectively exhibited high capacity of lipid (45.38% in dry weight) and starch (46.19% in dry weight) accumulation, which could be of high potential as feedstocks for biodiesel and bioethanol production. PMID:27598569

  20. Sustainable Process Design of Biofuels: Bioethanol Production from Cassava rhizome

    OpenAIRE

    Mangnimit, S.; Malakul, P.; Gani, Rafiqul

    2013-01-01

    This study is focused on the sustainable process design of bioethanol production from cassava rhizome. The study includes: process simulation, sustainability analysis, economic evaluation and life cycle assessment (LCA). A steady state process simulation if performed to generate a base case design of the bioethanol conversion process using cassava rhizome as a feedstock. The sustainability analysis is performed to analyze the relevant indicators in sustainability metrics, todefinedesign/retro...

  1. CO2 capture and biofuels production with microalgae

    International Nuclear Information System (INIS)

    Microalgae cultivation in large open ponds is the only biological process capable of directly utilizing power plant flue gas CO2 for production of renewable fuels, such as biodiesel, thus mitigating the potential for global warming. Past and recent systems studies have concluded that in principle this concept could be economically feasible, but that this technology still requires both fundamental and applied long-term R ampersand D

  2. Consequences of agro-biofuel production for greenhouse gas emissions

    OpenAIRE

    Carter, Mette S.; Hauggaard-Nielsen, Henrik; Johansen, Anders; Ambus, Per

    2009-01-01

    Currently CO2 from fossil fuel combustion accounts for 57% of the global greenhouse gas emissions, whereas the strong greenhouse gases nitrous oxide (N2O) and methane (CH4) contribute with 8% and 14%, respectively (IPCC, 2007). Agricultural activity is the dominant source of N2O, which is mainly associated with the use of nitrogen based fertilizers in agricultural production. Replacing fossil fuel-derived energy by biomass-derived energy is commonly and with increasing emphasis proposed as a ...

  3. CO{sub 2} capture and biofuels production with microalgae

    Energy Technology Data Exchange (ETDEWEB)

    Benemann, J.R. [Univ. of California, Berkeley, CA (United States)

    1995-11-01

    Microalgae cultivation in large open ponds is the only biological process capable of directly utilizing power plant flue gas CO{sub 2} for production of renewable fuels, such as biodiesel, thus mitigating the potential for global warming. Past and recent systems studies have concluded that in principle this concept could be economically feasible, but that this technology still requires both fundamental and applied long-term R&D.

  4. Techno-Economic Analysis of Biofuels Production Based on Gasification

    Energy Technology Data Exchange (ETDEWEB)

    Swanson, R. M.; Platon, A.; Satrio, J. A.; Brown, R. C.; Hsu, D. D.

    2010-11-01

    This study compares capital and production costs of two biomass-to-liquid production plants based on gasification. The first biorefinery scenario is an oxygen-fed, low-temperature (870?C), non-slagging, fluidized bed gasifier. The second scenario is an oxygen-fed, high-temperature (1,300?C), slagging, entrained flow gasifier. Both are followed by catalytic Fischer-Tropsch synthesis and hydroprocessing to naphtha-range (gasoline blend stock) and distillate-range (diesel blend stock) liquid fractions. Process modeling software (Aspen Plus) is utilized to organize the mass and energy streams and cost estimation software is used to generate equipment costs. Economic analysis is performed to estimate the capital investment and operating costs. Results show that the total capital investment required for nth plant scenarios is $610 million and $500 million for high-temperature and low-temperature scenarios, respectively. Product value (PV) for the high-temperature and low-temperature scenarios is estimated to be $4.30 and $4.80 per gallon of gasoline equivalent (GGE), respectively, based on a feedstock cost of $75 per dry short ton. Sensitivity analysis is also performed on process and economic parameters. This analysis shows that total capital investment and feedstock cost are among the most influential parameters affecting the PV.

  5. CHARACTERIZATION AND IDENTIFICATION OF FRESHWATER MICROALGAL STRAINS TOWARD BIOFUEL PRODUCTION

    Directory of Open Access Journals (Sweden)

    Xun Yang,

    2011-12-01

    Full Text Available Fifty-three algal cultures were isolated from freshwater lakes in Hainan, China. Four microalgal isolates were selected because they could be successfully cultivated at high density and demostrated a strong fluorescence after being stained with nile red. These cultures were identified as strains of Chlorella sp. C11, Chlamydomonas reinhardtii C22, Monoraphidium dybowskii C29, and Chlorella sp. HK12 through microscopic and 18S rDNA analysis. Under similar conditions, the lipid productivity of Chlorella sp. C11, Chla. reinhardtii C22, M. dybowskii C29 , and Chlorella sp. HK12 were 1.88, 2.79, 2.00, and 3.25 g L-1, respectively. Chla. reinhardtii C22 yielded a higher lipid content (51%, with a lower biomass concentration (5.47 g dwt L-1. Chlorella sp. HK12 reached a growth rate of 0.88 day-1 at OD540nm and yielded a biomass concentration of 7.56 g dwt L-1, with a high lipid content of 43%. Gas chromatography/ mass spectrometry analysis indicated that lipid fraction mainly comprises hydrocarbons including palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acids. Our results suggest that Chlorella sp. HK12 is a promising species for biodiesel production, because of its high lipid productivity and a relatively high content of oleic acid.

  6. Bio-Fuel Production Assisted with High Temperature Steam Electrolysis

    Energy Technology Data Exchange (ETDEWEB)

    Grant Hawkes; James O' Brien; Michael McKellar

    2012-06-01

    Two hybrid energy processes that enable production of synthetic liquid fuels that are compatible with the existing conventional liquid transportation fuels infrastructure are presented. Using biomass as a renewable carbon source, and supplemental hydrogen from high-temperature steam electrolysis (HTSE), these two hybrid energy processes have the potential to provide a significant alternative petroleum source that could reduce dependence on imported oil. The first process discusses a hydropyrolysis unit with hydrogen addition from HTSE. Non-food biomass is pyrolyzed and converted to pyrolysis oil. The pyrolysis oil is upgraded with hydrogen addition from HTSE. This addition of hydrogen deoxygenates the pyrolysis oil and increases the pH to a tolerable level for transportation. The final product is synthetic crude that could then be transported to a refinery and input into the already used transportation fuel infrastructure. The second process discusses a process named Bio-Syntrolysis. The Bio-Syntrolysis process combines hydrogen from HTSE with CO from an oxygen-blown biomass gasifier that yields syngas to be used as a feedstock for synthesis of liquid synthetic crude. Conversion of syngas to liquid synthetic crude, using a biomass-based carbon source, expands the application of renewable energy beyond the grid to include transportation fuels. It can also contribute to grid stability associated with non-dispatchable power generation. The use of supplemental hydrogen from HTSE enables greater than 90% utilization of the biomass carbon content which is about 2.5 times higher than carbon utilization associated with traditional cellulosic ethanol production. If the electrical power source needed for HTSE is based on nuclear or renewable energy, the process is carbon neutral. INL has demonstrated improved biomass processing prior to gasification. Recyclable biomass in the form of crop residue or energy crops would serve as the feedstock for this process. A process model

  7. Biofuels: which interest, which perspectives?

    International Nuclear Information System (INIS)

    This paper is a synthesis of several studies concerning the production and utilization of bio-fuels: energy balance and greenhouse effect of the various bio-fuel systems; economical analysis and profitability of bio-fuel production; is the valorization of bio-fuel residues and by-products in animal feeding a realistic hypothesis?; assessment of the cost for the community due to tax exemption for bio-fuels

  8. Regional Algal Biofuel Production Potential in the Coterminous United States as Affected by Resource Availability Trade-offs

    Energy Technology Data Exchange (ETDEWEB)

    Venteris, Erik R. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Skaggs, Richard [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Wigmosta, Mark S. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Coleman, Andre M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2014-03-15

    The warm sunny climate and unoccupied arid lands in the American southwest are favorable factors for algae cultivation. However, additional resources affect the overall viability of specific sites and regions. We investigated the tradeoffs between growth rate, water, and CO2 availability and costs for two strains: N. salina and Chlorella sp. We conducted site selection exercises (~88,000 US sites) to produce 21 billion gallons yr-1 (BGY) of renewable diesel (RD). Experimental trials from the National Alliance for Advanced Biofuels and Bio-Products (NAABB) team informed the growth model of our Biomass Assessment Tool (BAT). We simulated RD production by both lipid extraction and hydrothermal liquefaction. Sites were prioritized by the net value of biofuel minus water and flue gas costs. Water cost models for N. salina were based on seawater and high salinity groundwater and for Chlorella, fresh and brackish groundwater. CO2 costs were based on a flue gas delivery model. Selections constrained by production and water were concentrated along the Gulf of Mexico and southeast Atlantic coasts due to high growth rates and low water costs. Adding flue gas constraints increased the spatial distribution, but the majority of sites remained in the southeast. The 21 BGY target required ~3.8 million hectares of mainly forest (41.3%) and pasture (35.7%). Exclusion in favor of barren and scrub lands forced most production to the southwestern US, but with increased water consumption (5.7 times) and decreased economic efficiency (-38%).

  9. Panorama 2007: Biofuels Worldwide

    International Nuclear Information System (INIS)

    The biofuels market is booming: after more than 20 years of industrial development, global bio-fuel production is growing fast. Willingness to reduce their oil dependence and necessity to promote low-carbon energies are the two main drivers for states to support biofuels development. (author)

  10. Advances in metabolic engineering of yeast Saccharomyces cerevisiae for production of chemicals

    DEFF Research Database (Denmark)

    Borodina, Irina; Nielsen, Jens

    2014-01-01

    Yeast Saccharomyces cerevisiae is an important industrial host for production of enzymes, pharmaceutical and nutraceutical ingredients and recently also commodity chemicals and biofuels. Here, we review the advances in modeling and synthetic biology tools and how these tools can speed up the deve......Yeast Saccharomyces cerevisiae is an important industrial host for production of enzymes, pharmaceutical and nutraceutical ingredients and recently also commodity chemicals and biofuels. Here, we review the advances in modeling and synthetic biology tools and how these tools can speed up...... the development of yeast cell factories. We also present an overview of metabolic engineering strategies for developing yeast strains for production of polymer monomers: lactic, succinic, and cis,cis-muconic acids. S. cerevisiae has already firmly established itself as a cell factory in industrial biotechnology...... and the advances in yeast strain engineering will stimulate development of novel yeast-based processes for chemicals production....

  11. Consequences of agro-biofuel production for greenhouse gas emissions

    DEFF Research Database (Denmark)

    Carter, Mette Sustmann; Hauggaard-Nielsen, Henrik; Johansen, Anders;

    2009-01-01

    Currently CO2 from fossil fuel combustion accounts for 57% of the global greenhouse gas emissions, whereas the strong greenhouse gases nitrous oxide (N2O) and methane (CH4) contribute with 8% and 14%, respectively (IPCC, 2007). Agricultural activity is the dominant source of N2O, which is mainly...... as fertilizer for a maize energy crop within an organic cropping system. Furthermore, we assessed sustainability in terms of greenhouse gasses for co-production of bio-ethanol and bio-gas from maize. This was compared to estimated greenhouse gas balances for rye and grass-clover as alternative raw materials....

  12. An integrated renewable energy park approach for algal biofuel production in United States

    International Nuclear Information System (INIS)

    Algal biomass provides viable third generation feedstock for liquid transportation fuel that does not compete with food crops for cropland. However, fossil energy inputs and intensive water usage diminishes the positive aspects of algal energy production. An integrated renewable energy park (IREP) approach is proposed for aligning renewable energy industries in resource-specific regions in United States for synergistic electricity and liquid biofuel production from algal biomass with net zero carbon emissions. The benefits, challenges and policy needs of this approach are discussed.

  13. Tradeoffs and Synergies between biofuel production and large solar infrastructure in deserts.

    Science.gov (United States)

    Ravi, Sujith; Lobell, David B; Field, Christopher B

    2014-01-01

    Solar energy installations in deserts are on the rise, fueled by technological advances and policy changes. Deserts, with a combination of high solar radiation and availability of large areas unusable for crop production are ideal locations for large solar installations. However, for efficient power generation, solar infrastructures use large amounts of water for construction and operation. We investigated the water use and greenhouse gas (GHG) emissions associated with solar installations in North American deserts in comparison to agave-based biofuel production, another widely promoted potential energy source from arid systems. We determined the uncertainty in our analysis by a Monte Carlo approach that varied the most important parameters, as determined by sensitivity analysis. We considered the uncertainty in our estimates as a result of variations in the number of solar modules ha(-1), module efficiency, number of agave plants ha(-1), and overall sugar conversion efficiency for agave. Further, we considered the uncertainty in revenue and returns as a result of variations in the wholesale price of electricity and installation cost of solar photovoltaic (PV), wholesale price of agave ethanol, and cost of agave cultivation and ethanol processing. The life-cycle analyses show that energy outputs and GHG offsets from solar PV systems, mean energy output of 2405 GJ ha(-1) year(-1) (5 and 95% quantile values of 1940-2920) and mean GHG offsets of 464 Mg of CO2 equiv ha(-1) year(-1) (375-562), are much larger than agave, mean energy output from 206 (171-243) to 61 (50-71) GJ ha(-1) year(-1) and mean GHG offsets from 18 (14-22) to 4.6 (3.7-5.5) Mg of CO2 equiv ha(-1) year(-1), depending upon the yield scenario of agave. Importantly though, water inputs for cleaning solar panels and dust suppression are similar to amounts required for annual agave growth, suggesting the possibility of integrating the two systems to maximize the efficiency of land and water use to produce

  14. Quantifying the regional water footprint of biofuel production by incorporating hydrologic modeling

    Science.gov (United States)

    Wu, M.; Chiu, Y.; Demissie, Y.

    2012-10-01

    A spatially explicit life cycle water analysis framework is proposed, in which a standardized water footprint methodology is coupled with hydrologic modeling to assess blue water, green water (rainfall), and agricultural grey water discharge in the production of biofuel feedstock at county-level resolution. Grey water is simulated via SWAT, a watershed model. Evapotranspiration (ET) estimates generated with the Penman-Monteith equation and crop parameters were verified by using remote sensing results, a satellite-imagery-derived data set, and other field measurements. Crop irrigation survey data are used to corroborate the estimate of irrigation ET. An application of the concept is presented in a case study for corn-stover-based ethanol grown in Iowa (United States) within the Upper Mississippi River basin. Results show vast spatial variations in the water footprint of stover ethanol from county to county. Producing 1 L of ethanol from corn stover growing in the Iowa counties studied requires from 4.6 to 13.1 L of blue water (with an average of 5.4 L), a majority (86%) of which is consumed in the biorefinery. The county-level green water (rainfall) footprint ranges from 760 to 1000 L L-1. The grey water footprint varies considerably, ranging from 44 to 1579 L, a 35-fold difference, with a county average of 518 L. This framework can be a useful tool for watershed- or county-level biofuel sustainability metric analysis to address the heterogeneity of the water footprint for biofuels.

  15. Spatio-Temporal Impacts of Biofuel Production and Climate Variability on Water Quantity and Quality in Upper Mississippi River Basin

    OpenAIRE

    Debjani Deb; Pushpa Tuppad; Prasad Daggupati; Raghavan Srinivasan; Deepa Varma

    2015-01-01

    Impact of climate change on the water resources of the United States exposes the vulnerability of feedstock-specific mandated fuel targets to extreme weather conditions that could become more frequent and intensify in the future. Consequently, a sustainable biofuel policy should consider: (a) how climate change would alter both water supply and demand; and (b) in turn, how related changes in water availability will impact the production of biofuel crops; and (c) the environmental implications...

  16. Primary productivity and the prospects for biofuels in the United Kingdom

    Science.gov (United States)

    Lawson, G. J.; Callaghan, T. V.

    1983-09-01

    Estimates of land use and plant productivity are combined to predict total annual primary production in the UK as 252 million tonnes dry matter (10.5 t ha-1yr-1). Annual above ground production is predicted to be 165 Mt (6.9 t ha-1yr-1). Within these totals, intensive agriculture contributes 60%, productive woodland 8%, natural vegetation 26% and urban vegetation 5%. However, only 25% of total plant production is cropped by man and animals, and most of this is subsequently discarded as wastes and residues. 2112 PJ of organic material is available for fuel without reducing food or fibre production, but since much of this could not be economically collected, 859 PJ is calculated as a more realistic biofuel contribution by the year 2000. After deducting 50% conversion losses, this could save P1 billion (1979 prices) in oil imports. Short rotation energy plantations, forest residues, coppice woodlands, animal and crop wastes, industrial and domestic wastes, catch crops, natural vegetation and urban vegetation all have immediate or short term potential as biofuel sources. Sensitive planning is required to reduce environmental impact, but in some cases more diverse wildlife habitats may be created.

  17. Ultrasonic waste activated sludge disintegration for recovering multiple nutrients for biofuel production.

    Science.gov (United States)

    Xie, Guo-Jun; Liu, Bing-Feng; Wang, Qilin; Ding, Jie; Ren, Nan-Qi

    2016-04-15

    Waste activated sludge is a valuable resource containing multiple nutrients, but is currently treated and disposed of as an important source of pollution. In this work, waste activated sludge after ultrasound pretreatment was reused as multiple nutrients for biofuel production. The nutrients trapped in sludge floc were transferred into liquid medium by ultrasonic disintegration during first 30 min, while further increase of pretreatment time only resulted in slight increase of nutrients release. Hydrogen production by Ethanoligenens harbinense B49 from glucose significantly increased with the concentration of ultrasonic sludge, and reached maximum yield of 1.97 mol H2/mol glucose at sludge concentration of 7.75 g volatile suspended solids/l. Without addition of any other chemicals, waste molasses rich in carbohydrate was efficiently turned into hydrogen with yield of 189.34 ml H2/g total sugar by E. harbinense B49 using ultrasonic sludge as nutrients. The results also showed that hydrogen production using pretreated sludge as multiple nutrients was higher than those using standard nutrients. Acetic acid produced by E. harbinense B49 together with the residual nutrients in the liquid medium were further converted into hydrogen (271.36 ml H2/g total sugar) by Rhodopseudomonas faecalis RLD-53 through photo fermentation, while ethanol was the sole end product with yield of 220.26 mg/g total sugar. Thus, pretreated sludge was an efficient nutrients source for biofuel production, which could replace the standard nutrients. This research provided a novel strategy to achieve environmental friendly sludge disposal and simultaneous efficient biofuel recovery from organic waste. PMID:26896823

  18. Microalgal carbohydrates: an overview of the factors influencing carbohydrates production, and of main bioconversion technologies for production of biofuels

    DEFF Research Database (Denmark)

    Markou, Giorgos; Angelidaki, Irini; Georgakakis, Dimitris

    2012-01-01

    research is the cultivation of microalgae for lipids production to generate biodiesel. However, there are several other biological or thermochemical conversion technologies, in which microalgal biomass could be used as substrate. However, the high protein content or the low carbohydrate content of the......Microalgal biomass seems to be a promising feedstock for biofuel generation. Microalgae have relative high photosynthetic efficiencies, high growth rates, and some species can thrive in brackish water or seawater and wastewater from the food- and agro-industrial sector. Today, the main interest in...... majority of the microalgal species might be a constraint for their possible use in these technologies. Moreover, in the majority of biomass conversion technologies, carbohydrates are the main substrate for production of biofuels. Nevertheless, microalgae biomass composition could be manipulated by several...

  19. Production of biofuels, limonene and pectin from citrus wastes.

    Science.gov (United States)

    Pourbafrani, Mohammad; Forgács, Gergely; Horváth, Ilona Sárvári; Niklasson, Claes; Taherzadeh, Mohammad J

    2010-06-01

    Production of ethanol, biogas, pectin and limonene from citrus wastes (CWs) by an integrated process was investigated. CWs were hydrolyzed by dilute-acid process in a pilot plant reactor equipped with an explosive drainage. Hydrolysis variables including temperature and residence time were optimized by applying a central composite rotatable experimental design (CCRD). The best sugar yield (0.41g/g of the total dry CWs) was obtained by dilute-acid hydrolysis at 150 degrees C and 6min residence time. At this condition, high solubilization of pectin present in the CWs was obtained, and 77.6% of total pectin content of CWs could be recovered by solvent recovery. Degree of esterification and ash content of produced pectin were 63.7% and 4.23%, respectively. In addition, the limonene of the CWs was effectively removed through flashing of the hydrolyzates into an expansion tank. The sugars present in the hydrolyzates were converted to ethanol using baker's yeast, while an ethanol yield of 0.43g/g of the fermentable sugars was obtained. Then, the stillage and the remaining solid materials of the hydrolyzed CWs were anaerobically digested to obtain biogas. In summary, one ton of CWs with 20% dry weight resulted in 39.64l ethanol, 45m(3) methane, 8.9l limonene, and 38.8kg pectin. PMID:20149643

  20. Understanding and engineering enzymes for enhanced biofuel production.

    Energy Technology Data Exchange (ETDEWEB)

    Simmons, Blake Alexander; Volponi, Joanne V.; Sapra, Rajat; Faulon, Jean-Loup Michel; Buffleben, George M.; Roe, Diana C.

    2009-01-01

    Today, carbon-rich fossil fuels, primarily oil, coal and natural gas, provide 85% of the energy consumed in the United States. The release of greenhouse gases from these fuels has spurred research into alternative, non-fossil energy sources. Lignocellulosic biomass is renewable resource that is carbon-neutral, and can provide a raw material for alternative transportation fuels. Plant-derived biomass contains cellulose, which is difficult to convert to monomeric sugars for production of fuels. The development of cost-effective and energy-efficient processes to transform the cellulosic content of biomass into fuels is hampered by significant roadblocks, including the lack of specifically developed energy crops, the difficulty in separating biomass components, the high costs of enzymatic deconstruction of biomass, and the inhibitory effect of fuels and processing byproducts on organisms responsible for producing fuels from biomass monomers. One of the main impediments to more widespread utilization of this important resource is the recalcitrance of cellulosic biomass and techniques that can be utilized to deconstruct cellulosic biomass.

  1. Isolation and Characterization of a Marine Microalga for Biofuel Production with Astaxanthin as a Co-Product

    OpenAIRE

    Zhiyong Liu; Chenfeng Liu; Yuyong Hou; Shulin Chen; Dongguang Xiao; Juankun Zhang; Fangjian Chen

    2013-01-01

    Microalgae have been considered as a promising biomass for biofuel production, but freshwater resource consumption during the scaled-up cultivation are still a challenge. Obtaining robust marine strains capable of producing triacylglycerols and high value-added metabolites are critical for overcoming the limitations of water resources and economical feasibility. In this study, a marine microalga with lipid and astaxanthin accumulation capability was isolated from Bohai Bay, China. The alga wa...

  2. Unconventional biomasses as feedstocks for production of biofuels and succinic acid in a biorefinery concept

    DEFF Research Database (Denmark)

    Gunnarsson, Ingólfur Bragi

    production mainly due to their high carbohydrate content. A case study of a proposed macroalgae biorefinery concept highlighted the potential of post hydrolysis solid residue (PHSR) for the production of numerous additional products such as ω-3 and ω-6 fatty acids, biodiesel, protein, feed, biogas......Biorefinery has the potential of displacing fossil fuels and oil-refinery based products. Within the biorefinery a palette of marketable commodities can be produced from biomass, including food, feed, biochemicals and biofuels. Which bioproducts are produced is largely dependent on the chemical....... The chemical composition of biomasses was determined in order to demonstrate their biorefinery potential. Bioethanol and biogas along with succinic acid production were the explored bioconversion routes, while potential production of other compounds was also investigated. Differences and changes in biomass...

  3. Limits to biofuels

    Directory of Open Access Journals (Sweden)

    Johansson S.

    2013-06-01

    Full Text Available Biofuel production is dependent upon agriculture and forestry systems, and the expectations of future biofuel potential are high. A study of the global food production and biofuel production from edible crops implies that biofuel produced from edible parts of crops lead to a global deficit of food. This is rather well known, which is why there is a strong urge to develop biofuel systems that make use of residues or products from forest to eliminate competition with food production. However, biofuel from agro-residues still depend upon the crop production system, and there are many parameters to deal with in order to investigate the sustainability of biofuel production. There is a theoretical limit to how much biofuel can be achieved globally from agro-residues and this amounts to approximately one third of todays’ use of fossil fuels in the transport sector. In reality this theoretical potential may be eliminated by the energy use in the biomass-conversion technologies and production systems, depending on what type of assessment method is used. By surveying existing studies on biofuel conversion the theoretical limit of biofuels from 2010 years’ agricultural production was found to be either non-existent due to energy consumption in the conversion process, or up to 2–6000TWh (biogas from residues and waste and ethanol from woody biomass in the more optimistic cases.

  4. An Integrated Assessment of Location-Dependent Scaling for Microalgae Biofuel Production Facilities

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, Andre M.; Abodeely, Jared; Skaggs, Richard; Moeglein, William AM; Newby, Deborah T.; Venteris, Erik R.; Wigmosta, Mark S.

    2014-07-01

    Successful development of a large-scale microalgae-based biofuels industry requires comprehensive analysis and understanding of the feedstock supply chain—from facility siting/design through processing/upgrading of the feedstock to a fuel product. The evolution from pilot-scale production facilities to energy-scale operations presents many multi-disciplinary challenges, including a sustainable supply of water and nutrients, operational and infrastructure logistics, and economic competitiveness with petroleum-based fuels. These challenges are addressed in part by applying the Integrated Assessment Framework (IAF)—an integrated multi-scale modeling, analysis, and data management suite—to address key issues in developing and operating an open-pond facility by analyzing how variability and uncertainty in space and time affect algal feedstock production rates, and determining the site-specific “optimum” facility scale to minimize capital and operational expenses. This approach explicitly and systematically assesses the interdependence of biofuel production potential, associated resource requirements, and production system design trade-offs. The IAF was applied to a set of sites previously identified as having the potential to cumulatively produce 5 billion-gallons/year in the southeastern U.S. and results indicate costs can be reduced by selecting the most effective processing technology pathway and scaling downstream processing capabilities to fit site-specific growing conditions, available resources, and algal strains.

  5. The Effects of Biofuel Feedstock Production on Farmers’ Livelihoods in Ghana: The Case of Jatropha curcas

    Directory of Open Access Journals (Sweden)

    Emmanuel Acheampong

    2014-07-01

    Full Text Available The widespread acquisition of land for large-scale/commercial production of biofuel crops in Ghana has raised concerns from civil society organizations, local communities and other parties, regarding the impact of these investments on local livelihoods. This paper assessed the effect of large-scale acquisition of land for production of Jatropha curcas on farmers’ livelihoods in Ghana. The study was conducted in 11 communities spanning the major agro-ecological zones and political divisions across Ghana. Methods of data collection included questionnaire survey, interviews and focus group discussions. Results show that several households have lost their land to Jatropha plantations leading, in some cases, to violent conflicts between biofuel investors, traditional authorities and the local communities. Most people reported that, contrary to the belief that Jatropha does well on marginal lands, the lands acquired by the Jatropha Companies were productive lands. Loss of rights over land has affected households’ food production and security, as many households have resorted to reducing the area they have under cultivation, leading to shortening fallow periods and declining crop yields. In addition, although the cultivation of Jatropha led to the creation of jobs in the communities where they were started, such jobs were merely transient. The paper contends that, even though the impact of Jatropha feedstock production on local livelihoods in Ghana is largely negative, the burgeoning industry could be developed in ways that could support local livelihoods.

  6. New Biofuel Alternatives: Integrating Waste Management and Single Cell Oil Production

    Directory of Open Access Journals (Sweden)

    Elia Judith Martínez

    2015-04-01

    Full Text Available Concerns about greenhouse gas emissions have increased research efforts into alternatives in bio-based processes. With regard to transport fuel, bioethanol and biodiesel are still the main biofuels used. It is expected that future production of these biofuels will be based on processes using either non-food competing biomasses, or characterised by low CO2 emissions. Many microorganisms, such as microalgae, yeast, bacteria and fungi, have the ability to accumulate oils under special culture conditions. Microbial oils might become one of the potential feed-stocks for biodiesel production in the near future. The use of these oils is currently under extensive research in order to reduce production costs associated with the fermentation process, which is a crucial factor to increase economic feasibility. An important way to reduce processing costs is the use of wastes as carbon sources. The aim of the present review is to describe the main aspects related to the use of different oleaginous microorganisms for lipid production and their performance when using bio-wastes. The possibilities for combining hydrogen (H2 and lipid production are also explored in an attempt for improving the economic feasibility of the process.

  7. Impacts of biofuels production alternatives on water quantity and quality in the Iowa River Basin

    Science.gov (United States)

    Wu, Y.; Liu, S.

    2012-01-01

    Corn stover as well as perennial grasses like switchgrass (Panicum virgatum) and miscanthus are being considered as candidates for the second generation biofuel feedstocks. However, the challenges to biofuel development are its effects on the environment, especially water quality. This study evaluates the long-term impacts of biofuel production alternatives (e.g., elevated corn stover removal rates and the potential land cover change) on an ecosystem with a focus on biomass production, soil erosion, water quantity and quality, and soil nitrate nitrogen concentration at the watershed scale. The Soil and Water Assessment Tool (SWAT) was modified for setting land cover change scenarios and applied to the Iowa River Basin (a tributary of the Upper Mississippi River Basin). Results show that biomass production can be sustained with an increased stover removal rate as long as the crop demand for nutrients is met with appropriate fertilization. Although a drastic increase (4.7–70.6%) in sediment yield due to erosion and a slight decrease (1.2–3.2%) in water yield were estimated with the stover removal rate ranging between 40% and 100%, the nitrate nitrogen load declined about 6–10.1%. In comparison to growing corn, growing either switchgrass or miscanthus can reduce sediment erosion greatly. However, land cover changes from native grass to switchgrass or miscanthus would lead to a decrease in water yield and an increase in nitrate nitrogen load. In contrast to growing switchgrass, growing miscanthus is more productive in generating biomass, but its higher water demand may reduce water availability in the study area.

  8. Marine plants for CO{sub 2} remediation and biofuel production

    Energy Technology Data Exchange (ETDEWEB)

    Gao, K. [Shantou University, Shantou (China). Inst. of Energy and Environmental Science

    1996-12-31

    Biomass production from macroalgae has been viewed as important mainly because of the need for pollution abatement. This report shows that macroalgae have great potential for biomass production and CO{sub 2} bioremediation. Macroalgae have high productivity, as great or greater than the most productive land plants, and do not compete with terrestrial crops for farm land. This paper reviews recent data on macroalgal productivity and their biological responses to high CO{sub 2} concentrations, and discusses the feasibility of using macroalgae for bio-fuels. Biomass from macroalgae promises to provide environmentally and economically feasible alternatives to fossil fuels. Nevertheless, the techniques and technologies for growing macroalgae on a large-scale and for converting feedstocks to energy carriers must be more fully developed. 23 refs.

  9. Recent progress in supercritical fluid science for biofuel production from woody biomass

    Institute of Scientific and Technical Information of China (English)

    Shiro Saka

    2006-01-01

    Owing to an environment-friendly utilization of resources, increased attention has been focused on fuels and chemicals from biomass as an alternative to fossil resources. In addition, supercritical fluid technology has been considered to be an environmentally-benign treatment. Therefore, its technology was applied for a conversion of biomass to useful fuels and chemicals in order to mitigate environmental loading. For example, supercritical water treatment has demonstrated that lignocellulosics can be hydrolyzed to become lignin-derived products for useful aromatic chemicals and carbohydrate-derived products, such as polysaccharides,oligosaccharides and monosaccharides of glucose, mannose and xylose used for subsequent ethanol fermentation. If this treatment is prolonged, lignocellulosics were found to be converted to organic acids such as formic, acetic, glycolic and lactic acids which can be converted to methane for biofuel. When alcohols, such as methanol and ethanol, were used instead of water, some other useful products were achieved, and its liquefied products were found to have a potential for liquid biofuel. In this study, therefore, our research achievements in supercritical fluid science of woody biomass will be introduced for clean and green chemistry for a sustainable environment.

  10. Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production

    Directory of Open Access Journals (Sweden)

    Khalid M. Abu-Salah

    2013-05-01

    Full Text Available The isolation of lipid-rich autochthonous strains of microalgae is a crucial stage for the development of a microalgae-based biofuel production plant, as these microalgae already have the necessary adaptations to withstand competition, predation and the temperatures observed at each production site. This is particularly important in extreme climates such as in Saudi Arabia. Resorting to fluorescence activated cell sorting (FACS we screened for and isolated several microalgal strains from samples collected from the Red Sea. Relying on the fluorescence of BODIPY 505/515 (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diazasindacene and growth performance, four promising candidates were identified and the total lipid content and fatty acid profile was assessed for biofuels production. Selected isolates were classified as chlorophytes, belonging to three different genera: Picochlorum, Nannochloris and Desmochloris. The lipid contents were assessed microscopically by means of BODIPY 505/515-associated fluorescence to detect intracellular lipid bodies, which revealed several lipid drops in all selected strains. This result was confirmed by lipid gravimetric determination, which demonstrated that all strains under study presented inner cell lipid contents ranging from 20% to 25% of the biomass dry weight. Furthermore, the fatty acid methyl esters profile of all strains seems ideal for biodiesel production due to a low degree of polyunsaturated fatty acid methyl esters and high amount of palmitic and oleic acids.

  11. Quantifying the climate impacts of albedo changes due to biofuel production: a comparison with biogeochemical effects

    International Nuclear Information System (INIS)

    Lifecycle analysis is a tool widely used to evaluate the climate impact of greenhouse gas emissions attributable to the production and use of biofuels. In this paper we employ an augmented lifecycle framework that includes climate impacts from changes in surface albedo due to land use change. We consider eleven land-use change scenarios for the cultivation of biomass for middle distillate fuel production, and compare our results to previous estimates of lifecycle greenhouse gas emissions for the same set of land-use change scenarios in terms of CO2e per unit of fuel energy. We find that two of the land-use change scenarios considered demonstrate a warming effect due to changes in surface albedo, compared to conventional fuel, the largest of which is for replacement of desert land with salicornia cultivation. This corresponds to 222 gCO2e/MJ, equivalent to 3890% and 247% of the lifecycle GHG emissions of fuels derived from salicornia and crude oil, respectively. Nine of the land-use change scenarios considered demonstrate a cooling effect, the largest of which is for the replacement of tropical rainforests with soybean cultivation. This corresponds to − 161 gCO2e/MJ, or − 28% and − 178% of the lifecycle greenhouse gas emissions of fuels derived from soybean and crude oil, respectively. These results indicate that changes in surface albedo have the potential to dominate the climate impact of biofuels, and we conclude that accounting for changes in surface albedo is necessary for a complete assessment of the aggregate climate impacts of biofuel production and use. (paper)

  12. Quantifying the climate impacts of albedo changes due to biofuel production: a comparison with biogeochemical effects

    Science.gov (United States)

    Caiazzo, Fabio; Malina, Robert; Staples, Mark D.; Wolfe, Philip J.; Yim, Steve H. L.; Barrett, Steven R. H.

    2014-01-01

    Lifecycle analysis is a tool widely used to evaluate the climate impact of greenhouse gas emissions attributable to the production and use of biofuels. In this paper we employ an augmented lifecycle framework that includes climate impacts from changes in surface albedo due to land use change. We consider eleven land-use change scenarios for the cultivation of biomass for middle distillate fuel production, and compare our results to previous estimates of lifecycle greenhouse gas emissions for the same set of land-use change scenarios in terms of CO2e per unit of fuel energy. We find that two of the land-use change scenarios considered demonstrate a warming effect due to changes in surface albedo, compared to conventional fuel, the largest of which is for replacement of desert land with salicornia cultivation. This corresponds to 222 gCO2e/MJ, equivalent to 3890% and 247% of the lifecycle GHG emissions of fuels derived from salicornia and crude oil, respectively. Nine of the land-use change scenarios considered demonstrate a cooling effect, the largest of which is for the replacement of tropical rainforests with soybean cultivation. This corresponds to - 161 gCO2e/MJ, or - 28% and - 178% of the lifecycle greenhouse gas emissions of fuels derived from soybean and crude oil, respectively. These results indicate that changes in surface albedo have the potential to dominate the climate impact of biofuels, and we conclude that accounting for changes in surface albedo is necessary for a complete assessment of the aggregate climate impacts of biofuel production and use.

  13. Catalytic processes towards the production of biofuels in a palm oil and oil palm biomass-based biorefinery.

    Science.gov (United States)

    Chew, Thiam Leng; Bhatia, Subhash

    2008-11-01

    In Malaysia, there has been interest in the utilization of palm oil and oil palm biomass for the production of environmental friendly biofuels. A biorefinery based on palm oil and oil palm biomass for the production of biofuels has been proposed. The catalytic technology plays major role in the different processing stages in a biorefinery for the production of liquid as well as gaseous biofuels. There are number of challenges to find suitable catalytic technology to be used in a typical biorefinery. These challenges include (1) economic barriers, (2) catalysts that facilitate highly selective conversion of substrate to desired products and (3) the issues related to design, operation and control of catalytic reactor. Therefore, the catalytic technology is one of the critical factors that control the successful operation of biorefinery. There are number of catalytic processes in a biorefinery which convert the renewable feedstocks into the desired biofuels. These include biodiesel production from palm oil, catalytic cracking of palm oil for the production of biofuels, the production of hydrogen as well as syngas from biomass gasification, Fischer-Tropsch synthesis (FTS) for the conversion of syngas into liquid fuels and upgrading of liquid/gas fuels obtained from liquefaction/pyrolysis of biomass. The selection of catalysts for these processes is essential in determining the product distribution (olefins, paraffins and oxygenated products). The integration of catalytic technology with compatible separation processes is a key challenge for biorefinery operation from the economic point of view. This paper focuses on different types of catalysts and their role in the catalytic processes for the production of biofuels in a typical palm oil and oil palm biomass-based biorefinery. PMID:18434141

  14. Projecting future grassland productivity to assess the sustainability of potential biofuel feedstock areas in the Greater Platte River Basin

    Science.gov (United States)

    Gu, Yingxin; Wylie, Bruce K.; Boyte, Stephen; Phyual, Khem

    2014-01-01

    This study projects future (e.g., 2050 and 2099) grassland productivities in the Greater Platte River Basin (GPRB) using ecosystem performance (EP, a surrogate for measuring ecosystem productivity) models and future climate projections. The EP models developed from a previous study were based on the satellite vegetation index, site geophysical and biophysical features, and weather and climate drivers. The future climate data used in this study were derived from the National Center for Atmospheric Research Community Climate System Model 3.0 ‘SRES A1B’ (a ‘middle’ emissions path). The main objective of this study is to assess the future sustainability of the potential biofuel feedstock areas identified in a previous study. Results show that the potential biofuel feedstock areas (the more mesic eastern part of the GPRB) will remain productive (i.e., aboveground grassland biomass productivity >2750 kg ha−1 year−1) with a slight increasing trend in the future. The spatially averaged EPs for these areas are 3519, 3432, 3557, 3605, 3752, and 3583 kg ha−1 year−1 for current site potential (2000–2008 average), 2020, 2030, 2040, 2050, and 2099, respectively. Therefore, the identified potential biofuel feedstock areas will likely continue to be sustainable for future biofuel development. On the other hand, grasslands identified as having no biofuel potential in the drier western part of the GPRB would be expected to stay unproductive in the future (spatially averaged EPs are 1822, 1691, 1896, 2306, 1994, and 2169 kg ha−1 year−1 for site potential, 2020, 2030, 2040, 2050, and 2099). These areas should continue to be unsuitable for biofuel feedstock development in the future. These future grassland productivity estimation maps can help land managers to understand and adapt to the expected changes in future EP in the GPRB and to assess the future sustainability and feasibility of potential biofuel feedstock areas.

  15. Highly Efficient Process for Production of Biofuel from Ethanol Catalyzed by Ruthenium Pincer Complexes.

    Science.gov (United States)

    Xie, Yinjun; Ben-David, Yehoshoa; Shimon, Linda J W; Milstein, David

    2016-07-27

    A highly efficient ruthenium pincer-catalyzed Guerbet-type process for the production of biofuel from ethanol has been developed. It produces the highest conversion of ethanol (73.4%, 0.02 mol% catalyst) for a Guerbet-type reaction, including significant amounts of C4 (35.8% yield), C6 (28.2% yield), and C8 (9.4% yield) alcohols. Catalyst loadings as low as 0.001 mol% can be used, leading to a record turnover number of 18 209. Mechanistic studies reveal the likely active ruthenium species and the main deactivation process. PMID:27399841

  16. Biofuels from E. Coli: Engineering E. coli as an Electrofuels Chassis for Isooctane Production

    Energy Technology Data Exchange (ETDEWEB)

    None

    2010-07-16

    Electrofuels Project: Ginkgo Bioworks is bypassing photosynthesis and engineering E. coli to directly use carbon dioxide (CO2) to produce biofuels. E. coli doesn’t naturally metabolize CO2, but Ginkgo Bioworks is manipulating and incorporating the genes responsible for CO2 metabolism into the microorganism. By genetically modifying E. coli, Ginkgo Bioworks will enhance its rate of CO2 consumption and liquid fuel production. Ginkgo Bioworks is delivering CO2 to E. coli as formic acid, a simple industrial chemical that provides energy and CO2 to the bacterial system.

  17. Versatile microbial surface-display for environmental remediation and biofuels production

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Cindy H.; Mulchandani, Ashok; Chen, wilfred

    2008-02-14

    Surface display is a powerful technique that utilizes natural microbial functional components to express proteins or peptides on the cell exterior. Since the reporting of the first surface-display system in the mid-1980s, a variety of new systems have been reported for yeast, Gram-positive and Gram-negative bacteria. Non-conventional display methods are emerging, eliminating the generation of genetically modified microorganisms. Cells with surface display are used as biocatalysts, biosorbents and biostimulants. Microbial cell-surface display has proven to be extremely important for numerous applications ranging from combinatorial library screening and protein engineering to bioremediation and biofuels production.

  18. Isolation and Characterization of a Marine Microalga for Biofuel Production with Astaxanthin as a Co-Product

    Directory of Open Access Journals (Sweden)

    Shulin Chen

    2013-05-01

    Full Text Available Microalgae have been considered as a promising biomass for biofuel production, but freshwater resource consumption during the scaled-up cultivation are still a challenge. Obtaining robust marine strains capable of producing triacylglycerols and high value-added metabolites are critical for overcoming the limitations of water resources and economical feasibility. In this study, a marine microalga with lipid and astaxanthin accumulation capability was isolated from Bohai Bay, China. The alga was named as Coelastrum sp. HA-1 based on its morphological and molecular identification. The major characteristics of HA-1 and the effects of nitrogen on its lipid and astaxanthin accumulations were investigated. Results indicated that the highest biomass, lipid and astaxanthin yields achieved were 50.9 g m−2 day−1, 18.0 g m−2 day−1 and 168.9 mg m−2 day−1, respectively, after cultivation for 24 days. The fatty acids of HA-1, identified in their majority as oleic acid (56.6% and palmitic acid (25.9%, are desirable biofuel feedstocks. In addition, this alga can be harvested with simple sedimentation, achieving 98.2% removal efficiency after settling for 24 h. These results suggest that Coelastrum sp. HA-1 has several desirable key features that make it a potential candidate for biofuel production.

  19. Microalgal carbohydrates. An overview of the factors influencing carbohydrates production, and of main bioconversion technologies for production of biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Markou, Giorgos; Georgakakis, Dimitris [Agricultural Univ. of Athens (Greece). Dept. of Natural Resources Management and Agricultural Engineering; Angelidaki, Irini [Technical Univ. of Denmark, Lyngby (Denmark). Dept. of Environmental Engineering

    2012-11-15

    Microalgal biomass seems to be a promising feedstock for biofuel generation. Microalgae have relative high photosynthetic efficiencies, high growth rates, and some species can thrive in brackish water or seawater and wastewater from the food- and agro-industrial sector. Today, the main interest in research is the cultivation of microalgae for lipids production to generate biodiesel. However, there are several other biological or thermochemical conversion technologies, in which microalgal biomass could be used as substrate. However, the high protein content or the low carbohydrate content of the majority of the microalgal species might be a constraint for their possible use in these technologies. Moreover, in the majority of biomass conversion technologies, carbohydrates are the main substrate for production of biofuels. Nevertheless, microalgae biomass composition could be manipulated by several cultivation techniques, such as nutrient starvation or other stressed environmental conditions, which cause the microalgae to accumulate carbohydrates. This paper attempts to give a general overview of techniques that can be used for increasing the microalgal biomass carbohydrate content. In addition, biomass conversion technologies, related to the conversion of carbohydrates into biofuels are discussed. (orig.)

  20. Advanced uncooled sensor product development

    Science.gov (United States)

    Kennedy, A.; Masini, P.; Lamb, M.; Hamers, J.; Kocian, T.; Gordon, E.; Parrish, W.; Williams, R.; LeBeau, T.

    2015-06-01

    The partnership between RVS, Seek Thermal and Freescale Semiconductor continues on the path to bring the latest technology and innovation to both military and commercial customers. The partnership has matured the 17μm pixel for volume production on the Thermal Weapon Sight (TWS) program in efforts to bring advanced production capability to produce a low cost, high performance product. The partnership has developed the 12μm pixel and has demonstrated performance across a family of detector sizes ranging from formats as small as 206 x 156 to full high definition formats. Detector pixel sensitivities have been achieved using the RVS double level advanced pixel structure. Transition of the packaging of microbolometers from a traditional die level package to a wafer level package (WLP) in a high volume commercial environment is complete. Innovations in wafer fabrication techniques have been incorporated into this product line to assist in the high yield required for volume production. The WLP seal yield is currently > 95%. Simulated package vacuum lives >> 20 years have been demonstrated through accelerated life testing where the package has been shown to have no degradation after 2,500 hours at 150°C. Additionally the rugged assembly has shown no degradation after mechanical shock and vibration and thermal shock testing. The transition to production effort was successfully completed in 2014 and the WLP design has been integrated into multiple new production products including the TWS and the innovative Seek Thermal commercial product that interfaces directly to an iPhone or android device.

  1. Field emissions of N2O during biomass production may affect the sustainability of agro-biofuels

    DEFF Research Database (Denmark)

    Carter, Mette Sustmann; Hauggaard-Nielsen, Henrik; Heiske, Stefan;

    relate measured field emissions of N2O to the reduction in fossil fuel‐derived CO2, which is obtained when agricultural biomasses are used for biofuel production. The analysis includes five organically managed crops (viz. maize, rye, rye‐vetch, vetch and grass‐clover) and three scenarios for conversion...... of biomass to biofuel. The scenarios are 1) bioethanol, 2) biogas and 3) co‐production of bioethanol and biogas. In scenarios 3, the biomass is first used for bioethanol fermentation and subsequently the residue from this process is utilized for biogas production. The net reduction in greenhouse gas...... that field emissions of N2O contribute significantly to total greenhouse gas emissions during biofuel production, and in terms of global warming N2O emissions may counterbalance a considerable part of the avoided fossil CO2 emissions that are achieved by fossil fuel displacement. In the present study, we...

  2. Land use competition for production of food and liquid biofuels. An analysis of the arguments in the current debate

    International Nuclear Information System (INIS)

    This article analyses the current state of the debate over competition for land use, by means of an index of the main arguments in favor and against the production of liquid biofuels and the impacts on food production. Based on this index, an analytic framework is constructed to establish the causal relations indicated by the existing studies on this competition. We find that the emergence of agro-energy has altered the land use dynamic, albeit not yet significantly, with a shift of areas traditionally used to grow foods over to crops to produce biofuels. This has been contributing to raise food prices in the short run. However, it is probable that this is not the only factor determining this trend, nor will it last over the long run. The challenge is to conciliate the production of biofuels with the production of foods in sustainable form. (author)

  3. Effects of Escherichia coli on mixotrophic growth of Chlorella minutissima and production of biofuel precursors.

    Directory of Open Access Journals (Sweden)

    Brendan T Higgins

    Full Text Available Chlorella minutissima was co-cultured with Escherichia coli in airlift reactors under mixotrophic conditions (glucose, glycerol, and acetate substrates to determine possible effects of bacterial contamination on algal biofuel production. It was hypothesized that E. coli would compete with C. minutissima for nutrients, displacing algal biomass. However, C. minutissima grew more rapidly and to higher densities in the presence of E. coli, suggesting a symbiotic relationship between the organisms. At an initial 1% substrate concentration, the co-culture produced 200-587% more algal biomass than the axenic C. minutissima cultures. Co-cultures grown on 1% substrate consumed 23-737% more of the available carbon substrate than the sum of substrate consumed by E. coli and C. minutissima alone. At 1% substrate, total lipid and starch productivity were elevated in co-cultures compared to axenic cultures indicating that bacterial contamination was not detrimental to the production of biofuel precursors in this specific case. Bio-fouling of the reactors observed in co-cultures and acid formation in all mixotrophic cultures, however, could present challenges for scale-up.

  4. Biorefinery developments for advanced biofuels from a widening array of biomass feedstocks

    Science.gov (United States)

    When the United States passed the Renewable Fuel Standards (RFS) of 2007 into law it mandated that, by the year 2022, 36 billion gallons of biofuels be produced annually in the U.S. to displace petroleum. This targeted quota, which required that at least half of domestic transportation fuel be “adva...

  5. Exploring and integrating cellulolytic systems of insects to advance biofuel technology

    Institute of Scientific and Technical Information of China (English)

    Jian-Zhong Sun; Michael E. Scharf

    2010-01-01

    @@ In line with the requirements for sustainable economics and clean environments, cellulose-based biofuels have recently received tremendous attention both in industry and academic communities worldwide.Alternative and renewable fuels derived from lignocellulosic biomass of-fer the potential to reduce our dependence on fossil fuels and mitigate global climate change.

  6. Environmental impact assessment of biofuel production on contaminated land - Swedish case studies

    Energy Technology Data Exchange (ETDEWEB)

    Andersson-Skoeld, Yvonne; Suer, Pascal (Swedish Geotechnical Institute, Linkoeping (Sweden)); Blom, Sonja (FB Engineering AB, Goeteborg (Sweden)); Bardos, Paul (r3 Environmental Technology Ltd, Reading (United Kingdom)); Track, Thomas; Polland, Marcel (DECHEMA e. V., Frankfurt am Main (Germany))

    2009-07-01

    This report studies the (possible) cultivation of short rotation wood (Salix Vinimalis) on two contaminated sites from an environmental perspective, through a life cycle analysis (LCA) and carbon footprint, with an outlook towards an overarching method for a qualitative or semi-quantitative analysis based on a life cycle framework. Two areas were selected as case studies: a small site where short rotation crop (Salix Vinimalis) cultivation is in progress and a large site where biofuel production is hypothetical. For the selection of suitable sites, the following aspects were considered: Site location and size, so that biofuel cultivation might be economically viable without a remediation bonus, Topography and soil conditions, so that machinery could be used for cultivation, Time, so that the site was not in urgent need of remediation due to environmental or human health risks, or acute exploitation requirements, Contamination degree, which should not be plant-toxic, Contamination depth, Assessment of optimum crop and its use. For doubtful areas, it is especially important to analyse what the most viable option for the contaminated site is, and what bio-product could be used. For a more comprehensive analysis, which also incorporates local economic and social aspects, the decision support matrix, inter alia, described in the main report of the project Rejuvenate, is recommended. The calculation of emissions for the LCA and the carbon footprint used a German software tool for LCA of soil remediation. The software includes equipment emission data published in 1995. The module 'landfarming' has been used in this study to calculate emissions from herbicide application, fertilisation, ploughing and deep-ploughing, Salix harvest, harrowing etc. Since production of herbicide and Salix Vinimalis shoots were not included in the software, they were not included in the study. The conclusions for the two sites were very similar, in spite of the large differences

  7. Bio-fuel co-products in France: perspectives and consequences for cattle food; Coproduits des biocarburants en France: perspectives et consequences en alimentation animale

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    The development of bio-fuels goes along with that of co-products which can be used to feed animals. After having recalled the political context which promotes the development of renewable energies, this document aims at giving an overview of the impact of bio-fuel co-products on agriculture economy. It discusses the production and price evolution for different crops

  8. Production of algal-based biofuel using non-fresh water sources.

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Amy Cha-Tien; Reno, Marissa Devan

    2007-09-01

    The goal of this LDRD involves development of a system dynamics model to understand the interdependencies between water resource availability and water needs for production of biofuels. Specifically, this model focuses on availability and feasibility of non-traditional water sources from dairy wastewater, produced water from crude oil production and from coal-bed methane gas extraction for the production of algal-based biofuel. The conceptual simulation framework and historical data are based on two locales within New Mexico, the San Juan basin in the northwest and the Permian basin in the southeast, where oil and gas drilling have increased considerably in the last ten years. The overall water balance ignores both transportation options and water chemistry and is broken down by county level. The resulting model contains an algal growth module, a dairy module, an oil production module, and a gas production module. A user interface is also created for controlling the adjustable parameters in the model. Our preliminary investigation indicates a cyclical demand for non-fresh water due to the cyclical nature of algal biomass production and crop evapotranspiration. The wastewater from the dairy industry is not a feasible non-fresh water source because the agricultural water demand for cow's dry feed far exceeds the amount generated at the dairy. The uncertainty associated with the water demand for cow's dry matter intake is the greatest in this model. The oil- and gas-produced water, ignoring the quality, provides ample supply for water demand in algal biomass production. There remains work to address technical challenges associated with coupling the appropriate non-fresh water source to the local demand.

  9. Optimization of Biofuel and Biochar Production from the Slow Pyrolysis of Biomass

    Science.gov (United States)

    Fang, J.; Gao, B.; Nsf Reu in Water Resources

    2010-12-01

    Slow pyrolysis was performed on biomass samples (i.e., energy cane and air potato) to determine the most energy efficient conditions for producing biofuel and biochar. The potential of air potato as a source of fuel and char was also investigated. Dry biomass samples of 10, 15 and 20 g were heated in a reactor at a final temperatures of 300, 450, or 600 °C, and the minimum amount of time required to complete pyrolysis was recorded. Maximum biochar yield was obtained at 300°C for both energy cane and air potato at all masses, and maximum bio-oil yield was obtained at 450°C for all samples. Pyrolysis required the least amount of time at 450°C. Bio-oil yields for air potato were slightly lower than that of energy cane, while biochar yield was slightly higher. Since air potato showed similar product yields to energy cane, this indicates it has potential to be a good feedstock for biofuel and biochar productions.

  10. Biomass production of multipopulation microalgae in open air pond for biofuel potential.

    Science.gov (United States)

    Selvakumar, P; Umadevi, K

    2016-04-01

    Biodiesel gains attention as it is made from renewable resources and has considerable environmental benefits. The present investigation has focused on large scale cultivation of multipopulation microalgae in open air pond using natural sea water without any additional nutritive supplements for low cost biomass production as a possible source of biofuel in large scale. Open air algal pond attained average chlorophyll concentration of 11.01 µg/L with the maximum of 43.65 µg/L as well as a higher lipid concentration of 18% (w/w) with lipid content 9.3 mg/L on the 10th day of the culture; and maximum biomass of 0.36 g/L on the 7th day of the culture. Composition analysis of fatty acid methyl ester (FAME) was performed by gas chromatography and mass spectrometry (GCMS). Multipopulation of algal biomass had 18% of total lipid content with 55% of total saturated fatty acids (SFA), 35.3% of monounsaturated fatty acids (MUFA) and 9.7% of polyunsaturated fatty acids (PUFA), revealing a potential source of biofuel production at low cost.

  11. Comparative genomics of xylose-fermenting fungi for enhanced biofuel production

    Energy Technology Data Exchange (ETDEWEB)

    Wohlbach, Dana J.; Kuo, Alan; Sato, Trey K.; Potts, Katlyn M.; Salamov, Asaf A.; LaButti, Kurt M.; Sun, Hui; Clum, Alicia; Pangilinan, Jasmyn L.; Lindquist, Erika A.; Lucas, Susan; Lapidus, Alla; Jin, Mingjie; Gunawan, Christa; Balan, Venkatesh; Dale, Bruce E.; Jeffries, Thomas W.; Zinkel, Robert; Barry, Kerrie W.; Grigoriev, Igor V.; Gasch, Audrey P.

    2011-02-24

    Cellulosic biomass is an abundant and underused substrate for biofuel production. The inability of many microbes to metabolize the pentose sugars abundant within hemicellulose creates specific challenges for microbial biofuel production from cellulosic material. Although engineered strains of Saccharomyces cerevisiae can use the pentose xylose, the fermentative capacity pales in comparison with glucose, limiting the economic feasibility of industrial fermentations. To better understand xylose utilization for subsequent microbial engineering, we sequenced the genomes of two xylose-fermenting, beetle-associated fungi, Spathaspora passalidarum and Candida tenuis. To identify genes involved in xylose metabolism, we applied a comparative genomic approach across 14 Ascomycete genomes, mapping phenotypes and genotypes onto the fungal phylogeny, and measured genomic expression across five Hemiascomycete species with different xylose-consumption phenotypes. This approach implicated many genes and processes involved in xylose assimilation. Several of these genes significantly improved xylose utilization when engineered into S. cerevisiae, demonstrating the power of comparative methods in rapidly identifying genes for biomass conversion while reflecting on fungal ecology.

  12. Biomass production of multipopulation microalgae in open air pond for biofuel potential.

    Science.gov (United States)

    Selvakumar, P; Umadevi, K

    2016-04-01

    Biodiesel gains attention as it is made from renewable resources and has considerable environmental benefits. The present investigation has focused on large scale cultivation of multipopulation microalgae in open air pond using natural sea water without any additional nutritive supplements for low cost biomass production as a possible source of biofuel in large scale. Open air algal pond attained average chlorophyll concentration of 11.01 µg/L with the maximum of 43.65 µg/L as well as a higher lipid concentration of 18% (w/w) with lipid content 9.3 mg/L on the 10th day of the culture; and maximum biomass of 0.36 g/L on the 7th day of the culture. Composition analysis of fatty acid methyl ester (FAME) was performed by gas chromatography and mass spectrometry (GCMS). Multipopulation of algal biomass had 18% of total lipid content with 55% of total saturated fatty acids (SFA), 35.3% of monounsaturated fatty acids (MUFA) and 9.7% of polyunsaturated fatty acids (PUFA), revealing a potential source of biofuel production at low cost. PMID:27295924

  13. Comparative proteomics analysis of engineered Saccharomyces cerevisiae with enhanced biofuel precursor production.

    Directory of Open Access Journals (Sweden)

    Xiaoling Tang

    Full Text Available The yeast Saccharomyces cerevisiae was metabolically modified for enhanced biofuel precursor production by knocking out genes encoding mitochondrial isocitrate dehydrogenase and over-expression of a heterologous ATP-citrate lyase. A comparative iTRAQ-coupled 2D LC-MS/MS analysis was performed to obtain a global overview of ubiquitous protein expression changes in S. cerevisiae engineered strains. More than 300 proteins were identified. Among these proteins, 37 were found differentially expressed in engineered strains and they were classified into specific categories based on their enzyme functions. Most of the proteins involved in glycolytic and pyruvate branch-point pathways were found to be up-regulated and the proteins involved in respiration and glyoxylate pathway were however found to be down-regulated in engineered strains. Moreover, the metabolic modification of S. cerevisiae cells resulted in a number of up-regulated proteins involved in stress response and differentially expressed proteins involved in amino acid metabolism and protein biosynthesis pathways. These LC-MS/MS based proteomics analysis results not only offered extensive information in identifying potential protein-protein interactions, signal pathways and ubiquitous cellular changes elicited by the engineered pathways, but also provided a meaningful biological information platform serving further modification of yeast cells for enhanced biofuel production.

  14. Microalgae Recovery from Water for Biofuel Production Using CO2-Switchable Crystalline Nanocellulose.

    Science.gov (United States)

    Ge, Shijian; Champagne, Pascale; Wang, Haidong; Jessop, Philip G; Cunningham, Michael F

    2016-07-19

    There is a pressing need to develop efficient and sustainable approaches to harvesting microalgae for biofuel production and water treatment. CO2-switchable crystalline nanocellulose (CNC) modified with 1-(3-aminopropyl)imidazole (APIm) is proposed as a reversible coagulant for harvesting microalgae. Compared to native CNC, the positively charged APIm-modified CNC, which dispersed well in carbonated water, showed appreciable electrostatic interaction with negatively charged Chlorella vulgaris upon CO2-treatment. The gelation between the modified CNC, triggered by subsequent air sparging, can also enmesh adjacent microalgae and/or microalgae-modified CNC aggregates, thereby further enhancing harvesting efficiencies. Moreover, the surface charges and dispersion/gelation of APIm-modified CNC could be reversibly adjusted by alternatively sparging CO2/air. This CO2-switchability would make the reusability of redispersed CNC for further harvesting possible. After harvesting, the supernatant following sedimentation can be reused for microalgal cultivation without detrimental effects on cell growth. The use of this approach for harvesting microalgae presents an advantage to other current methods available because all materials involved, including the cellulose, CO2, and air, are natural and biocompatible without adverse effects on the downstream processing for biofuel production. PMID:27314988

  15. Simulated moving bed separation of agarose-hydrolyzate components for biofuel production from marine biomass.

    Science.gov (United States)

    Kim, Pung-Ho; Nam, Hee-Geun; Park, Chanhun; Wang, Nien-Hwa Linda; Chang, Yong Keun; Mun, Sungyong

    2015-08-01

    The economically-efficient separation of galactose, levulinic acid (LA), and 5-hydroxymethylfurfural (5-HMF) in acid hydrolyzate of agarose has been a key issue in the area of biofuel production from marine biomass. To address this issue, an optimal simulated moving bed (SMB) process for continuous separation of the three agarose-hydrolyzate components with high purities, high yields, and high throughput was developed in this study. As a first step for this task, the adsorption isotherm and mass-transfer parameters of each component on the qualified adsorbent were determined through a series of multiple frontal experiments. The determined parameters were then used in optimizing the SMB process for the considered separation. Finally, the optimized SMB process was tested experimentally using a self-assembled SMB unit with four zones. The SMB experimental results and the relevant computer simulations verified that the developed process in this study was quite successful in the economically-efficient separation of galactose, LA, and 5-HMF in a continuous mode with high purities and high yields. It is thus expected that the developed SMB process in this study will be able to serve as one of the trustworthy ways of improving the economic feasibility of biofuel production from marine biomass. PMID:26141276

  16. Utilisation of biofuels from forest products residues in the periphery of Eastern Macedonia and Thrace - Greece

    International Nuclear Information System (INIS)

    In the framework of inquiry of biomass usage possibilities in our country, a research was made through the whole Eastern Macedonia region, related to disposal possibilities from the production conveyors and usage possibilities from the region's residents and businessmen, of bio-fuels that come from mechanical wood treatment residues and from the residues of agricultural production, in wooden form, known with the term wood pellets or wood briquettes. From the present study came to light that there is an intense interest for direct personal usage and application of biomass for the residents or occupational spaces heating. The necessary biomass quantity for all the region's households is estimated up to 205.520 tons. From the biomass usage as a combustible material a 680 €/year and household about, is made. The potential investments that will be made to the region seem to have a very shortly depreciation, but a business plan and a marketing plan syntax are considered as necessary for the result's maximization. Key Words: biofuels, wood pellets, wood briquettes, periphery of Eastern Macedonia and Thrace. (author)

  17. Hydrocracking of vacuum gas oil-vegetable oil mixtures for biofuels production.

    Science.gov (United States)

    Bezergianni, Stella; Kalogianni, Aggeliki; Vasalos, Iacovos A

    2009-06-01

    Hydrocracking of vacuum gas oil (VGO)--vegetable oil mixtures is a prominent process for the production of biofuels. In this work both pre-hydrotreated and non-hydrotreated VGO are assessed whether they are suitable fossil components in a VGO-vegetable oil mixture as feed-stocks to a hydrocracking process. This assessment indicates the necessity of a VGO pre-hydrotreated step prior to hydrocracking the VGO-vegetable oil mixture. Moreover, the comparison of two different mixing ratios suggests that higher vegetable oil content favors hydrocracking product yields and qualities. Three commercial catalysts of different activity are utilized in order to identify a range of products that can be produced via a hydrocracking route. Finally, the effect of temperature on hydrocracking VGO-vegetable oil mixtures is studied in terms of conversion and selectivity to diesel, jet/kerosene and naphtha.

  18. Thermodynamic analysis of lignocellulosic biofuel production via a biochemical process: guiding technology selection and research focus.

    Science.gov (United States)

    Sohel, M Imroz; Jack, Michael W

    2011-02-01

    The aim of this paper is to present an exergy analysis of bioethanol production process from lignocellulosic feedstock via a biochemical process to asses the overall thermodynamic efficiency and identify the main loss processes. The thermodynamic efficiency of the biochemical process was found to be 35% and the major inefficiencies of this process were identified as: the combustion of lignin for process heat and power production and the simultaneous scarification and co-fermentation process accounting for 67% and 27% of the lost exergy, respectively. These results were also compared with a previous analysis of a thermochemical process for producing biofuel. Despite fundamental differences, the biochemical and thermochemical processes considered here had similar levels of thermodynamic efficiency. Process heat and power production was the major contributor to exergy loss in both of the processes. Unlike the thermochemical process, the overall efficiency of the biochemical process largely depends on how the lignin is utilized.

  19. Carbon Calculator for Land Use Change from Biofuels Production (CCLUB). Users' Manual and Technical Documentation

    Energy Technology Data Exchange (ETDEWEB)

    Dunn, Jennifer B. [Argonne National Lab. (ANL), Argonne, IL (United States); Qin, Zhangcai [Argonne National Lab. (ANL), Argonne, IL (United States); Mueller, Steffen [Univ. of Illinois at Chicago, Chicago, IL (United States); Kwon, Ho-young [International Food Policy Research Institute (IFPRI), Washington, DC (United States); Wander, Michelle M. [Univ. of Illinois, Urbana-Champaign, IL (United States); Wang, Michael [Argonne National Lab. (ANL), Argonne, IL (United States)

    2014-09-01

    The Carbon Calculator for Land Use Change from Biofuels Production (CCLUB) calculates carbon emissions from land use change (LUC) for four different ethanol production pathways including corn grain ethanol and cellulosic ethanol from corn stover, Miscanthus, and switchgrass. This document discusses the version of CCLUB released September 30, 2014 which includes corn and three cellulosic feedstocks: corn stover, Miscanthus, and switchgrass.

  20. Classification, mode of action and production strategy of xylanase and its application for biofuel production from water hyacinth.

    Science.gov (United States)

    Uday, Uma Shankar Prasad; Choudhury, Payel; Bandyopadhyay, Tarun Kanti; Bhunia, Biswanath

    2016-01-01

    Xylanases are classified under glycoside hydrolase families which represent one of the largest groups of commercial enzymes. Depolymerizing xylan molecules into monomeric pentose units involves the synergistic action of mainly two key enzymes which are endo-β-xylanase and β-xylosidase. Xylanases are different with respect to their mode of action, substrate specificities, biochemical properties, 3D structure and are widely produced by a spectrum of bacteria and fungi. Currently, large scale production of xylanase can be produced through the application of genetic engineering tool which allow fast identification of novel xylanase genes and their genetic variations makes it an ideal enzymes. Due to depletion of fossil fuel, there is urgent need to find out environment friendly and sustainable energy sources. Therefore, utilisation of cheap lignocellulosic materials along with proper optimisation of process is most important for cost efficient ethanol production. Among, various types of lignocellulosic substances, water hyacinth, a noxious aquatic weed, has been found in many tropical. Therefore, the technological development for biofuel production from water hyacinth is becoming commercially worthwhile. In this review, the classification and mode of action of xylanase including genetic regulation and strategy for robust xylanase production have been critically discussed from recent reports. In addition various strategies for cost effective biofuel production from water hyacinth including chimeric proteins design has also been critically evaluated.

  1. The impact of site preparation for biofuel and sawtimber production on soil compaction and long-term soil productivity

    Energy Technology Data Exchange (ETDEWEB)

    Leggett, Z.H.; Sucre, E.B. [Weyerhaeuser NR Co., Vanceboro, NC (United States)

    2010-07-01

    Research into renewable fuel sources has increased as a result of increasing global concerns over carbon dioxide (CO{sub 2}) emissions. This study assessed that potential effects of concurrent biofuel and high quality wood product production in forests along the lower coastal plain of North Carolina. The effects of intercropping and biomass management on site productivity and sustainability were investigated on loblolly pine plantations; loblolly pine and switchgrass; and loblolly pines with flat-planted pine trees planted between crop tree beds. Stands of switchgrass only were also investigated. The treatments were established on 0.81 hectare plots and replicated 4 times. The impacts of various non-traditional land management approaches on soil productivity and sustainability were assessed, as well as the site preparations needed to develop a dual crop system. The study showed no significant differences in soil compaction before and after the treatments were installed. Average soil resistance across all treatments at the soil surface was 201 kPa. Soil resistance increased to 1539 kPa and 1923 kPa at depths of 15 and 30 cm. The study indicated that dual crop systems and practices for biofuel and timber production are both economically feasible and environmentally sustainable.

  2. Microbial-Derived Oils and Value-Added Products: Biosynthesis and Applications for Biofuel Production

    OpenAIRE

    McCurdy, Alex T.

    2015-01-01

    Efforts are being made to replace petroleum-derived fuels with biofuels in a cost competitive manner. It is apparent that the continued use of petroleum is futile as population and technological growth put increasing pressure on the demand for cheap energy and chemicals. Diminishing resources, civil unrest in the Middle East, and the impact of using petrochemicals on the environment are critical driving forces for research in generating renewable petroleum replacements that can be produced wi...

  3. Greenhouse gas mitigation on marginal land: a quantitative review of the relative benefits of forest recovery versus biofuel production.

    Science.gov (United States)

    Evans, Samuel G; Ramage, Benjamin S; DiRocco, Tara L; Potts, Matthew D

    2015-02-17

    Decisions concerning future land-use/land cover change stand at the forefront of ongoing debates on how to best mitigate climate change. In this study, we compare the greenhouse gas (GHG) mitigation value over a 30-year time frame for a range of forest recovery and biofuel production scenarios on abandoned agricultural land. Carbon sequestration in recovering forests is estimated based on a statistical analysis of tropical and temperate studies on marginal land. GHGs offset by biofuel production are analyzed for five different production pathways. We find that forest recovery is superior to low-yielding biofuel production scenarios such as oil palm and corn. Biofuel production scenarios with high yields, such as sugarcane or high-yielding energy grasses, can be comparable or superior to natural forest succession and to reforestation in some cases. This result stands in contrast to previous research suggesting that restoring degraded ecosystems to their native state is generally superior to agricultural production in terms of GHG mitigation. Further work is needed on carbon stock changes in forests, soil carbon dynamics, and bioenergy crop production on degraded/abandoned agricultural land. This finding also emphasizes the need to consider the full range of social, economic, and ecological consequences of land-use policies. PMID:25582654

  4. Water Resources Implications of Cellulosic Biofuel Production at a Regional Scale

    Science.gov (United States)

    Christopher, S. F.; Schoenholtz, S. H.; Nettles, J. E.

    2011-12-01

    Recent increases in oil prices, a strong national interest in greater energy independence, and a concern for the role of fossil fuels in global climate change, have led to a dramatic expansion in use of alternative renewable energy sources in the U.S. The U.S. government has mandated production of 36 billion gallons of renewable fuels by 2022, of which 16 billion gallons are required to be cellulosic biofuels. Production of cellulosic biomass offers a promising alternative to corn-based systems because large-scale production of corn-based ethanol often requires irrigation and is associated with increased erosion, excess sediment export, and enhanced leaching of nitrogen and phosphorus. Although cultivation of switchgrass using standard agricultural practices is one option being considered for production of cellulosic biomass, intercropping cellulosic biofuel crops within managed forests could provide feedstock without primary land use change or the water quality impacts associated with annual crops. Catchlight Energy LLC is examining the feasibility and sustainability of intercropping switchgrass in loblolly pine plantations in the southeastern U.S. Ongoing research is determining efficient operational techniques and information needed to evaluate effects of these practices on water resources in small watershed-scale (~25 ha) studies. Three sets of four to five sub-watersheds are fully instrumented and currently collecting calibration data in North Carolina, Alabama, and Mississippi. These watershed studies will provide detailed information to understand processes and guide management decisions. However, environmental implications of cellulosic systems need to be examined at a regional scale. We used the Soil Water Assessment Tool (SWAT), a physically-based hydrologic model, to examine water quantity effects of various land use change scenarios ranging from switchgrass intercropping a small percentage of managed pine forest land to conversion of all managed

  5. Genetic Engineering of Energy Crops: A Strategy for Biofuel Production in China Free Access

    Institute of Scientific and Technical Information of China (English)

    Guosheng Xie; Liangcai Peng

    2011-01-01

    Biomass utilization is increasingly considered as a practical way for sustainable energy supply and long-term environment care around the world.In concerns with food security in China,starch or sugar-based bioethanol and edible-oil-derived biodiesel are harshly restricted for large scale production.However,conversion of lignocellulosic residues from food crops is a potential alternative.Because of its recalcitrance,current biomass process is unacceptably expensive,but genetic breeding of energy crops is a promising solution.To meet the need,energy crops are defined with a high yield for both food and biofuel purposes.In this review,main grasses(rice,wheat,maize,sorghum and miscanthus)are evaluated for high biomass production,the principles are discussed on modification of plant cell walls that lead to efficient biomass degradation and conversion,and the related biotechnologies are proposed in terms of energy crop selection.

  6. Biomass logistics analysis for large scale biofuel production: case study of loblolly pine and switchgrass.

    Science.gov (United States)

    Lu, Xiaoming; Withers, Mitch R; Seifkar, Navid; Field, Randall P; Barrett, Steven R H; Herzog, Howard J

    2015-05-01

    The objective of this study was to assess the costs, energy consumption and greenhouse gas (GHG) emissions throughout the biomass supply chain for large scale biofuel production. Two types of energy crop were considered, switchgrass and loblolly pine, as representative of herbaceous and woody biomass. A biomass logistics model has been developed to estimate the feedstock supply system from biomass production through transportation. Biomass in the form of woodchip, bale and pellet was investigated with road, railway and waterway transportation options. Our analysis indicated that the farm or forest gate cost is lowest for loblolly pine whole tree woodchip at $39.7/dry tonne and highest for switchgrass round bale at $72.3/dry tonne. Switchgrass farm gate GHG emissions is approximately 146kgCO2e/dry tonne, about 4 times higher than loblolly pine. The optimum biomass transportation mode and delivered form are determined by the tradeoff between fixed and variable costs for feedstock shipment.

  7. Novel approaches to microalgal and cyanobacterial cultivation for bioenergy and biofuel production.

    Science.gov (United States)

    Heimann, Kirsten

    2016-04-01

    Growing demand for energy and food by the global population mandates finding water-efficient renewable resources. Microalgae/cyanobacteria have shown demonstrated capacity to contribute to global energy and food security. Yet, despite proven process technology and established net energy-effectiveness and cost-effectiveness through co-product generation, microalgal biofuels are not a reality. This review outlines novel biofilm cultivation strategies that are water-smart, the opportunity for direct energy conversion via anaerobic digestion of N2-fixing cyanobacterial biomass and integrative strategies for microalgal biodiesel and/or biocrude production via supercritical methanol-direct transesterification and hydrothermal liquefaction, respectively. Additionally, fermentation of cyanobacterial biofilms could supply bioethanol to feed wet transesterification to biodiesel conversion for on-site use in remote locations.

  8. Novel approaches to microalgal and cyanobacterial cultivation for bioenergy and biofuel production.

    Science.gov (United States)

    Heimann, Kirsten

    2016-04-01

    Growing demand for energy and food by the global population mandates finding water-efficient renewable resources. Microalgae/cyanobacteria have shown demonstrated capacity to contribute to global energy and food security. Yet, despite proven process technology and established net energy-effectiveness and cost-effectiveness through co-product generation, microalgal biofuels are not a reality. This review outlines novel biofilm cultivation strategies that are water-smart, the opportunity for direct energy conversion via anaerobic digestion of N2-fixing cyanobacterial biomass and integrative strategies for microalgal biodiesel and/or biocrude production via supercritical methanol-direct transesterification and hydrothermal liquefaction, respectively. Additionally, fermentation of cyanobacterial biofilms could supply bioethanol to feed wet transesterification to biodiesel conversion for on-site use in remote locations. PMID:26953746

  9. Biomass logistics analysis for large scale biofuel production: case study of loblolly pine and switchgrass.

    Science.gov (United States)

    Lu, Xiaoming; Withers, Mitch R; Seifkar, Navid; Field, Randall P; Barrett, Steven R H; Herzog, Howard J

    2015-05-01

    The objective of this study was to assess the costs, energy consumption and greenhouse gas (GHG) emissions throughout the biomass supply chain for large scale biofuel production. Two types of energy crop were considered, switchgrass and loblolly pine, as representative of herbaceous and woody biomass. A biomass logistics model has been developed to estimate the feedstock supply system from biomass production through transportation. Biomass in the form of woodchip, bale and pellet was investigated with road, railway and waterway transportation options. Our analysis indicated that the farm or forest gate cost is lowest for loblolly pine whole tree woodchip at $39.7/dry tonne and highest for switchgrass round bale at $72.3/dry tonne. Switchgrass farm gate GHG emissions is approximately 146kgCO2e/dry tonne, about 4 times higher than loblolly pine. The optimum biomass transportation mode and delivered form are determined by the tradeoff between fixed and variable costs for feedstock shipment. PMID:25710677

  10. Environmental impact assessment of biofuel production on contaminated land - Swedish case studies

    Energy Technology Data Exchange (ETDEWEB)

    Andersson-Skoeld, Yvonne; Suer, Pascal (Swedish Geotechnical Institute, Linkoeping (Sweden)); Blom, Sonja (FB Engineering AB, Goeteborg (Sweden)); Bardos, Paul (r3 Environmental Technology Ltd, Reading (United Kingdom)); Track, Thomas; Polland, Marcel (DECHEMA e. V., Frankfurt am Main (Germany))

    2009-07-01

    This report studies the (possible) cultivation of short rotation wood (Salix Vinimalis) on two contaminated sites from an environmental perspective, through a life cycle analysis (LCA) and carbon footprint, with an outlook towards an overarching method for a qualitative or semi-quantitative analysis based on a life cycle framework. Two areas were selected as case studies: a small site where short rotation crop (Salix Vinimalis) cultivation is in progress and a large site where biofuel production is hypothetical. For the selection of suitable sites, the following aspects were considered: Site location and size, so that biofuel cultivation might be economically viable without a remediation bonus, Topography and soil conditions, so that machinery could be used for cultivation, Time, so that the site was not in urgent need of remediation due to environmental or human health risks, or acute exploitation requirements, Contamination degree, which should not be plant-toxic, Contamination depth, Assessment of optimum crop and its use. For doubtful areas, it is especially important to analyse what the most viable option for the contaminated site is, and what bio-product could be used. For a more comprehensive analysis, which also incorporates local economic and social aspects, the decision support matrix, inter alia, described in the main report of the project Rejuvenate, is recommended. The calculation of emissions for the LCA and the carbon footprint used a German software tool for LCA of soil remediation. The software includes equipment emission data published in 1995. The module 'landfarming' has been used in this study to calculate emissions from herbicide application, fertilisation, ploughing and deep-ploughing, Salix harvest, harrowing etc. Since production of herbicide and Salix Vinimalis shoots were not included in the software, they were not included in the study. The conclusions for the two sites were very similar, in spite of the large differences

  11. Assessing regional hydrology and water quality implications of large-scale biofuel feedstock production in the Upper Mississippi River Basin.

    Science.gov (United States)

    Demissie, Yonas; Yan, Eugene; Wu, May

    2012-08-21

    A recent U.S. Department of Energy study estimated that more than one billion tons of biofuel feedstock could be produced by 2030 in the United States from increased corn yield, and changes in agricultural and forest residue management and land uses. To understand the implications of such increased production on water resources and stream quality at regional and local scales, we have applied a watershed model for the Upper Mississippi River Basin, where most of the current and future crop/residue-based biofuel production is expected. The model simulates changes in water quality (soil erosion, nitrogen and phosphorus loadings in streams) and resources (soil-water content, evapotranspiration, and runoff) under projected biofuel production versus the 2006 baseline year and a business-as-usual scenario. The basin average results suggest that the projected feedstock production could change the rate of evapotranspiration in the UMRB by approximately +2%, soil-water content by about -2%, and discharge to streams by -5% from the baseline scenario. However, unlike the impacts on regional water availability, the projected feedstock production has a mixed effect on water quality, resulting in 12% and 45% increases in annual suspended sediment and total phosphorus loadings, respectively, but a 3% decrease in total nitrogen loading. These differences in water quantity and quality are statistically significant (p < 0.05). The basin responses are further analyzed at monthly time steps and finer spatial scales to evaluate underlying physical processes, which would be essential for future optimization of environmentally sustainable biofuel productions.

  12. Energy balance of biofuel production from biological conversion of crude glycerol.

    Science.gov (United States)

    Zhang, Xiaolei; Yan, Song; Tyagi, Rajeshwar D; Surampalli, Rao Y; Valéro, Jose R

    2016-04-01

    Crude glycerol, a by-product of biodiesel production, has gained significant attention as a carbon source for biofuel production. This study evaluated the energy balance of biodiesel, hydrogen, biogas, and ethanol production from 3.48 million L of crude glycerol (80% w/v). The conversion efficiency (energy output divided by energy invested) was 1.16, 0.22, 0.27, and 0.40 for the production of biodiesel, hydrogen, biogas, and ethanol respectively. It was found that the use of crude glycerol for biodiesel production was an energy gain process, with a positive energy balance and conversion efficiency of greater than 1. The energy balance revealed a net energy gain of 5226 GJ per 1 million kg biodiesel produced. Production of hydrogen, biogas and ethanol from crude glycerol were energy loss processes. Therefore, the conversion of crude glycerol to lipids and subsequently to biodiesel is suggested to be a better option compared to hydrogen, biogas, or ethanol production with respect to energy balance.

  13. Biofuel and other biomass based products from contaminated sites - Potentials and barriers from Swedish perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Andersson-Skoeld, Yvonne; Enell, Anja; Rihm, Thomas; Haglund, Kristina; Wik, Ola (Swedish Geotechnical Institute, Linkoeping (Sweden)); Blom, Sonja; Angelbratt, Alexandra (FB Engineering AB, Goeteborg (Sweden)); Bardos, Paul (r3 Environmental Technology Ltd, Reading (United Kingdom)); Track, Thomas (DECHEMA e. V., Frankfurt am Main (Germany)); Keuning, Sytze (Bioclear b.v., Groningen (Netherlands))

    2009-07-01

    In this report, results are presented based on interviews and literature surveys on the triggers and stoppers for non food crop on contaminated land in Sweden. The report also includes a first estimate of potential marginal land for biofuel production in Sweden. The report is a first step to explore the feasibility of a range of possible approaches to combine risk based land management (RBLM) with non-food crop land-uses and organic matter re-use as appropriate in a Swedish context. The focus of the report is on the treatment of contaminated land by phyto-remediation and on biofuel cultivation. In Sweden, like all other countries in Europe, areas of land have been degraded by past use. Such previously developed land includes areas affected by mining, fallout from industrial processes such as smelting, areas elevated with contaminated dredged sediments, former landfill sites and many other areas where the decline of industrial activity has left a legacy of degraded land and communities. The extent of contamination may not be sufficient to trigger remediation under current regulatory conditions, and there may be little economic incentive to regenerate the affected areas. An ideal solution would be a land management approach that is able to pay for itself. Biomass from coppice or other plantations has long been seen as a possible means of achieving this goal. Phyto remediation offers a low cost method for remediation of areas that are not candidates for conventional regeneration. The optimal conditions for phyto remediation are large land areas of low or mediate contamination. Phyto remediation is also suitable to prevent spreading of contaminants, for example in green areas such as in cities, as waste water buffer and small size remediation areas with diffuse spreading. Phyto remediation implies that plants, fungi or algae are used to remediate, control or increase the natural attenuation of contaminants. Depending on the contaminating species and the site conditions

  14. Characterization of microalga Nannochloropsis sp. mutants for improved production of biofuels

    International Nuclear Information System (INIS)

    Highlights: ► Growth/fatty acid content difference of Nannochloropsis sp and its’ wild type. ► Alga cultured with 1% CO2 in two light conditions 140 and 300 μmol photons m−2 s−1. ► Growth in biomass/chlorophyll/lipid content is higher in higher light conditions. ► Mutant LARB-202-3 had better growth and lipid content compared to other two mutants. ► The LARB-202-3 mutant is a candidate for feed stock for biofuels production. -- Abstract: To select microalgae with a high biomass, chlorophyll a, lipid, and fatty acid content of two mutants (LARB-202-2 and LARB-202-3) and their parent wild type of the unicellular green alga Nannochloropsis sp were cultured with air containing 1% CO2 for a week in 1 L bubbled tubes with continuous illumination at 140 or 300 μmol photons m−2 s−1. Overall biomass productivity of all three strains were higher under high light (HL, 300 μmol photons m−2 s−1) with LARB-202-3 achieving the highest volumetric productivity (0.9 g L−1 d−1) and parent wild type the lowest (0.72 g L−1 d−1) when cultured for 6 days with medium nitrogen level. Biomass productivity of all three strains were substantially low in response to low light (LL, 140 μmol photons m−2 s−1) growth conditions. However, LARB-202-3 showed the highest biomass productivity (0.74 g L−1 d−1) under LL conditions too. LARB-202-3 possessed high photosynthetic productivity as measured by chlorophyll a (Chl a) content under HL conditions throughout the growth period. The content of Chl a declined gradually in all three tested strains over time. Volumetric productivity of biomass was closely associated with the cellular content of Chl a. Total lipid productivity of LARB-202-2 and LARB-202-3 grown in low nitrogen media for 12 days were 273 and 297 mg L−1 d−1, respectively, while that of wild type parent was 244 mg L−1 d−1. Major medium chain fatty acids (e.g. C14:0 and C16:0) make up nearly 63% of total fatty acids in all three strains. No

  15. Biogeochemical Research Priorities for Sustainable Biofuel and Bioenergy Feedstock Production in the Americas

    Science.gov (United States)

    Gollany, Hero T.; Titus, Brian D.; Scott, D. Andrew; Asbjornsen, Heidi; Resh, Sigrid C.; Chimner, Rodney A.; Kaczmarek, Donald J.; Leite, Luiz F. C.; Ferreira, Ana C. C.; Rod, Kenton A.; Hilbert, Jorge; Galdos, Marcelo V.; Cisz, Michelle E.

    2015-12-01

    Rapid expansion in biomass production for biofuels and bioenergy in the Americas is increasing demand on the ecosystem resources required to sustain soil and site productivity. We review the current state of knowledge and highlight gaps in research on biogeochemical processes and ecosystem sustainability related to biomass production. Biomass production systems incrementally remove greater quantities of organic matter, which in turn affects soil organic matter and associated carbon and nutrient storage (and hence long-term soil productivity) and off-site impacts. While these consequences have been extensively studied for some crops and sites, the ongoing and impending impacts of biomass removal require management strategies for ensuring that soil properties and functions are sustained for all combinations of crops, soils, sites, climates, and management systems, and that impacts of biomass management (including off-site impacts) are environmentally acceptable. In a changing global environment, knowledge of cumulative impacts will also become increasingly important. Long-term experiments are essential for key crops, soils, and management systems because short-term results do not necessarily reflect long-term impacts, although improved modeling capability may help to predict these impacts. Identification and validation of soil sustainability indicators for both site prescriptions and spatial applications would better inform commercial and policy decisions. In an increasingly inter-related but constrained global context, researchers should engage across inter-disciplinary, inter-agency, and international lines to better ensure the long-term soil productivity across a range of scales, from site to landscape.

  16. Biogeochemical Research Priorities for Sustainable Biofuel and Bioenergy Feedstock Production in the Americas.

    Science.gov (United States)

    Gollany, Hero T; Titus, Brian D; Scott, D Andrew; Asbjornsen, Heidi; Resh, Sigrid C; Chimner, Rodney A; Kaczmarek, Donald J; Leite, Luiz F C; Ferreira, Ana C C; Rod, Kenton A; Hilbert, Jorge; Galdos, Marcelo V; Cisz, Michelle E

    2015-12-01

    Rapid expansion in biomass production for biofuels and bioenergy in the Americas is increasing demand on the ecosystem resources required to sustain soil and site productivity. We review the current state of knowledge and highlight gaps in research on biogeochemical processes and ecosystem sustainability related to biomass production. Biomass production systems incrementally remove greater quantities of organic matter, which in turn affects soil organic matter and associated carbon and nutrient storage (and hence long-term soil productivity) and off-site impacts. While these consequences have been extensively studied for some crops and sites, the ongoing and impending impacts of biomass removal require management strategies for ensuring that soil properties and functions are sustained for all combinations of crops, soils, sites, climates, and management systems, and that impacts of biomass management (including off-site impacts) are environmentally acceptable. In a changing global environment, knowledge of cumulative impacts will also become increasingly important. Long-term experiments are essential for key crops, soils, and management systems because short-term results do not necessarily reflect long-term impacts, although improved modeling capability may help to predict these impacts. Identification and validation of soil sustainability indicators for both site prescriptions and spatial applications would better inform commercial and policy decisions. In an increasingly inter-related but constrained global context, researchers should engage across inter-disciplinary, inter-agency, and international lines to better ensure the long-term soil productivity across a range of scales, from site to landscape. PMID:26006220

  17. Preliminary results on optimising hydrothermal treatment used in co-production of biofuels

    DEFF Research Database (Denmark)

    Thomsen, M.H.; Thomsen, A.B.; Jørgensen, H.;

    ) is performed to solubilize the hemicellulose fraction in straw, open the cellulose structure to increase accessibility of enzymes, and to remove the alkaline salts(e.g. potassium chloride). Alkaline salts cause corrosion problems in conventional boilers during incineration of straw for electricity production...... the pre-treatment and fermentation processeswill be concentrated and used for animal feed. Several trials were made with varying parameters of water level, chemical addition and flow in the reactor. All experiments were performed at 190 C, except for a single experiment performed at 200 C. Theresults...... illustrates that it is possible to extract more than 95% of the alkaline salts (at 200 C) leaving a solid cellulose rich biofuel for combustion or for further treatment in the ethanol process. In the experiments performed at 190 C, the best totalglucose yield after pre-treatment and following enzymatic...

  18. The Role of Symbiotic Nitrogen Fixation in Sustainable Production of Biofuels

    Directory of Open Access Journals (Sweden)

    Bandana Biswas

    2014-04-01

    Full Text Available With the ever-increasing population of the world (expected to reach 9.6 billion by 2050, and altered life style, comes an increased demand for food, fuel and fiber. However, scarcity of land, water and energy accompanied by climate change means that to produce enough to meet the demands is getting increasingly challenging. Today we must use every avenue from science and technology available to address these challenges. The natural process of symbiotic nitrogen fixation, whereby plants such as legumes fix atmospheric nitrogen gas to ammonia, usable by plants can have a substantial impact as it is found in nature, has low environmental and economic costs and is broadly established. Here we look at the importance of symbiotic nitrogen fixation in the production of biofuel feedstocks; how this process can address major challenges, how improving nitrogen fixation is essential, and what we can do about it.

  19. Effects of Environmental Factors and Nutrient Availability on the Biochemical Composition of Algae for Biofuels Production: A Review

    OpenAIRE

    Murthy, Ganti S.; Ruben Michael Ceballos; Ankita Juneja

    2013-01-01

    Due to significant lipid and carbohydrate production as well as other useful properties such as high production of useful biomolecular substrates (e.g., lipids) and the ability to grow using non-potable water sources, algae are being explored as a potential high-yield feedstock for biofuels production. In both natural and engineered systems, algae can be exposed to a variety of environmental conditions that affect growth rate and cellular composition. With respect to the latter, the amount of...

  20. Liquid diesel biofuel production in Sweden. A study of producers using forestry or agricultural sector feedstock

    Energy Technology Data Exchange (ETDEWEB)

    Andrae, Erik

    2009-06-15

    but respondents expressed that the market potential for their products was 'almost unlimited'. A couple of the firms had the option to produce synthetic diesel but were primarily focusing on other fuels that were more raw material efficient. The crude tall diesel production was considered to be more limited by available resources in Sweden than the production of synthetic diesel of cellulosic biomass. The firms using forestry sector feedstock were otherwise facing similar difficulties and mostly with the authorities. As they were getting more established they were expecting competition primarily with the petroleum diesel price. This could be solved by selling the fuels to customers with demands on low emission fuels or with environmental policies. The study was conducted by interviewing all the biodiesel producers in Sweden and several of the companies that were entering the industry of producing biofuels from cellulosic biomass. The firms were asked individually about their perceptions on producing liquid diesel biofuels and the prospects of these fuels in the future. This report is therefore based on their thoughts and comments and presents both a general picture of the industries and what aspects were important to them

  1. From 1st- to 2nd-Generation Biofuel Technologies: Extended Executive Summary

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    This report looks at the technical challenges facing 2nd-generation biofuels, evaluates their costs and examines related current policies to support their development and deployment. The potential for production of more advanced biofuels is also discussed. Although significant progress continues to be made to overcome the technical and economic challenges, 2nd-generation biofuels still face major constraints to their commercial deployment.

  2. Biofuels Baseline 2008

    Energy Technology Data Exchange (ETDEWEB)

    Hamelinck, C.; Koper, M.; Berndes, G.; Englund, O.; Diaz-Chavez, R.; Kunen, E.; Walden, D.

    2011-10-15

    The European Union is promoting the use of biofuels and other renewable energy in transport. In April 2009, the Renewable Energy Directive (2009/28/EC) was adopted that set a 10% target for renewable energy in transport in 2020. The directive sets several requirements to the sustainability of biofuels marketed in the frame of the Directive. The Commission is required to report to the European Parliament on a regular basis on a range of sustainability impacts resulting from the use of biofuels in the EU. This report serves as a baseline of information for regular monitoring on the impacts of the Directive. Chapter 2 discusses the EU biofuels market, the production and consumption of biofuels and international trade. It is derived where the feedstock for EU consumed biofuels originally come from. Chapter 3 discusses the biofuel policy framework in the EU and major third countries of supply. It looks at various policy aspects that are relevant to comply with the EU sustainability requirements. Chapter 4 discusses the environmental and social sustainability aspects associated with EU biofuels and their feedstock. Chapter 5 discusses the macro-economic effects that indirectly result from increased EU biofuels consumption, on commodity prices and land use. Chapter 6 presents country factsheets for main third countries that supplied biofuels to the EU market in 2008.

  3. Production of biofuels and biochemicals by in vitro synthetic biosystems: Opportunities and challenges.

    Science.gov (United States)

    Zhang, Yi-Heng Percival

    2015-11-15

    The largest obstacle to the cost-competitive production of low-value and high-impact biofuels and biochemicals (called biocommodities) is high production costs catalyzed by microbes due to their inherent weaknesses, such as low product yield, slow reaction rate, high separation cost, intolerance to toxic products, and so on. This predominant whole-cell platform suffers from a mismatch between the primary goal of living microbes - cell proliferation and the desired biomanufacturing goal - desired products (not cell mass most times). In vitro synthetic biosystems consist of numerous enzymes as building bricks, enzyme complexes as building modules, and/or (biomimetic) coenzymes, which are assembled into synthetic enzymatic pathways for implementing complicated bioreactions. They emerge as an alternative solution for accomplishing a desired biotransformation without concerns of cell proliferation, complicated cellular regulation, and side-product formation. In addition to the most important advantage - high product yield, in vitro synthetic biosystems feature several other biomanufacturing advantages, such as fast reaction rate, easy product separation, open process control, broad reaction condition, tolerance to toxic substrates or products, and so on. In this perspective review, the general design rules of in vitro synthetic pathways are presented with eight supporting examples: hydrogen, n-butanol, isobutanol, electricity, starch, lactate,1,3-propanediol, and poly-3-hydroxylbutyrate. Also, a detailed economic analysis for enzymatic hydrogen production from carbohydrates is presented to illustrate some advantages of this system and the remaining challenges. Great market potentials will motivate worldwide efforts from multiple disciplines (i.e., chemistry, biology and engineering) to address the remaining obstacles pertaining to cost and stability of enzymes and coenzymes, standardized building parts and modules, biomimetic coenzymes, biosystem optimization, and scale

  4. Brown rot fungal early stage decay mechanism as a biological pretreatment for softwood biomass in biofuel production

    Energy Technology Data Exchange (ETDEWEB)

    Ray, Michael J.; Leak, David J.; Spanu, Pietro D.; Murphy, Richard J. [Division of Biology, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ (United Kingdom); Porter Alliance, Imperial College London, London SW7 2AZ (United Kingdom)

    2010-08-15

    A current barrier to the large-scale production of lignocellulosic biofuels is the cost associated with the energy and chemical inputs required for feedstock pretreatment and hydrolysis. The use of controlled partial biological degradation to replace elements of the current pretreatment technologies would offer tangible energy and cost benefits to the whole biofuel process. It has been known for some time from studies of wood decay that, in the early stages of growth in wood, brown rot fungi utilise a mechanism that causes rapid and extensive depolymerisation of the carbohydrate polymers of the wood cell wall. The brown rot hyphae act as delivery vectors to the plant cell wall for what is thought to be a combination of a localised acid pretreatment and a hydroxyl radical based depolymerisation of the cell wall carbohydrate polymers. It is this quality that we have exploited in the present work to enhance the saccharification potential of softwood forest residues for biofuel production. Here we show that after restricted exposure of pine sapwood to brown rot fungi, glucose yields following enzymatic saccharification are significantly increased. Our results demonstrate the potential of using brown rot fungi as a biological pretreatment for biofuel production. (author)

  5. Switchgrass potential on reclaimed surface mines for biofuel production in West Virginia

    Science.gov (United States)

    Marra, Michael A.

    The high cost and environmental risks associated with non-renewable energy sources has caused an increased interest in, and development of renewable biofuels. Switchgrass (Panicum virgatum L.), a warm season perennial grass, has been investigated as a source of biofuel feedstock due to its high biomass production on marginal soils, its tolerance of harsh growing conditions, and its ability to provide habitat for wildlife and soil conservation cover. West Virginia contains vast expanses of reclaimed surface mine lands and could potentially benefit from the production of switchgrass as a biofuel feedstock. Furthermore, switchgrass production could satisfy Surface Mining Reclamation and Control Act of 1977 (SMCRA) requirements for reclamation bond release to mine operators. Three separate studies will be discussed in this thesis to determine if switchgrass grown on reclaimed surface mines can produce yields similar to yields from stands grown under normal agronomic conditions and what common surface mining reclamation practices may be most appropriate for growing switchgrass. The first study examined yield production of three commercially-available, upland switchgrass varieties grown on two reclaimed surface mines in production years two, three and four. The Hampshire Hill mine site, which was reclaimed in the late 1990s using top soil and treated municipal sludge, averaged 5,800 kg (ha-yr)-1 of switchgrass compared to 803 kg (ha-yr)-1 at the Hobet 21 site which was reclaimed with crushed, unweathered rock over compacted overburden. Site and variety interacted with Cave-in-Rock as the top performer at the more fertile Hampshire Hill site and Shawnee produced the highest yields at Hobet 21 (7,853 kg ha-1 and 1,086 kg ha-1 averaged across years, respectively). Switchgrass yields increased from 2009 to 2010, but declined from 2010 to 2011. Switchgrass yields from farmlands in this region averaged about 15000 kg (ha-yr)-1 in the research literature, so switchgrass grown

  6. Material property characterization of co-products from biofuel industries: Potential uses in value-added biocomposites

    International Nuclear Information System (INIS)

    This paper gives an insight of biofuel production and the status -into the co-products obtained from this industry. Furthermore this work explores the possibility of these co-products as raw materials for value-added uses in material applications. This is achieved by understanding composition, solid density, and moisture content of prominent co-products such as soy meal, DDGS (distillers’ dried grains with solubles) and jatropha meal. Moisture content and density measurements showed no trend. Soy meal has the highest protein content, followed by jatropha and DDGS. Thermal stability of these co-products was analyzed by thermogravimetric analysis (TGA), which revealed that the thermal stabilities are ranked as soy meal>DDGS>jatropha meal. FT-IR spectroscopy was used to understand the functional groups in these meals and it showed that the amide group was prominent in all of these meals. In pursuit of finding value-added uses for these co-products of biofuel industries, biodegradable polymer, i.e. polycaprolactone (PCL), based biocomposites were prepared by melt processing technique using extrusion followed by injection molding. Tensile, flexural and impact properties were evaluated. Also, scanning electron microscopy (SEM) of fractured sections of the biocomposites was examined. -- Highlights: ► This paper gives an insight of biofuel production and its co-products. ► We have characterized biofuel co-products such as soy meal, DDGS and jatropha meal. ► Thermal stability and functional groups of these co-products were determined. ► Polycaprolactone based biocomposites were prepared by melt processing technique. ► Tensile, flexural and impact properties of these biocomposites were evaluated.

  7. Addressing the challenges for sustainable production of algal biofuels: I. Algal strains and nutrient supply.

    Science.gov (United States)

    Abdelaziz, Ahmed E M; Leite, Gustavo B; Hallenbeck, Patrick C

    2013-01-01

    Microalgae hold promise for the production of sustainable replacement of fossil fuels due to their high growth rates, ability to grow on non-arable land and their high content, under the proper conditions, of high energy compounds that can be relatively easily chemically converted to fuels using existing technology. However, projected large-scale algal production raises a number of sustainability concerns concerning land use, net energy return, water use and nutrient supply. The state-of-the-art of algal production of biofuels is presented with emphasis on some possible avenues to provide answers to the sustainability questions that have been raised. Here, issues concerning algal strains and supply of nutrients for large-scale production are discussed. Since sustainability concerns necessitate the use of wastewaters for supply of bulk nutrients, emphasis is placed on the composition and suitability of different wastewater streams. At the same time, algal cultivation has proven useful in waste treatment processes, and thus this aspect is also treated in some detail. PMID:24350435

  8. Soil nutrient budgets following projected corn stover harvest for biofuel production in the conterminous United States

    Science.gov (United States)

    Tan, Zhengxi; Liu, Shuguang

    2015-01-01

    Increasing demand for food and biofuel feedstocks may substantially affect soil nutrient budgets, especially in the United States where there is great potential for corn (Zea mays L) stover as a biofuel feedstock. This study was designed to evaluate impacts of projected stover harvest scenarios on budgets of soil nitrogen (N), phosphorus (P), and potassium (K) currently and in the future across the conterminous United States. The required and removed N, P, and K amounts under each scenario were estimated on the basis of both their average contents in grain and stover and from an empirical model. Our analyses indicate a small depletion of soil N (−4 ± 35 kg ha−1) and K (−6 ± 36 kg ha−1) and a moderate surplus of P (37 ± 21 kg ha−1) currently on the national average, but with a noticeable variation from state to state. After harvesting both grain and projected stover, the deficits of soil N, P, and K were estimated at 114–127, 26–27, and 36–53 kg ha−1 yr−1, respectively, in 2006–2010; 131–173, 29–32, and 41–96 kg ha−1 yr−1, respectively, in 2020; and 161–207, 35–39, and 51–111 kg ha−1 yr−1, respectively, in 2050. This study indicates that the harvestable stover amount derived from the minimum stover requirement for maintaining soil organic carbon level scenarios under current fertilization rates can be sustainable for soil nutrient supply and corn production at present, but the deficit of P and K at the national scale would become larger in the future.

  9. %22Trojan Horse%22 strategy for deconstruction of biomass for biofuels production.

    Energy Technology Data Exchange (ETDEWEB)

    Simmons, Blake Alexander; Sinclair, Michael B.; Yu, Eizadora; Timlin, Jerilyn Ann; Hadi, Masood Z.; Tran-Gyamfi, Mary

    2011-02-01

    Production of renewable biofuels to displace fossil fuels currently consumed in the transportation sector is a pressing multiagency national priority (DOE/USDA/EERE). Currently, nearly all fuel ethanol is produced from corn-derived starch. Dedicated 'energy crops' and agricultural waste are preferred long-term solutions for renewable, cheap, and globally available biofuels as they avoid some of the market pressures and secondary greenhouse gas emission challenges currently facing corn ethanol. These sources of lignocellulosic biomass are converted to fermentable sugars using a variety of chemical and thermochemical pretreatments, which disrupt cellulose and lignin cross-links, allowing exogenously added recombinant microbial enzymes to more efficiently hydrolyze the cellulose for 'deconstruction' into glucose. This process is plagued with inefficiencies, primarily due to the recalcitrance of cellulosic biomass, mass transfer issues during deconstruction, and low activity of recombinant deconstruction enzymes. Costs are also high due to the requirement for enzymes and reagents, and energy-intensive cumbersome pretreatment steps. One potential solution to these problems is found in synthetic biology-engineered plants that self-produce a suite of cellulase enzymes. Deconstruction can then be integrated into a one-step process, thereby increasing efficiency (cellulose-cellulase mass-transfer rates) and reducing costs. The unique aspects of our approach are the rationally engineered enzymes which become Trojan horses during pretreatment conditions. During this study we rationally engineered Cazy enzymes and then integrated them into plant cells by multiple transformation techniques. The regenerated plants were assayed for first expression of these messages and then for the resulting proteins. The plants were then subjected to consolidated bioprocessing and characterized in detail. Our results and possible implications of this work on developing

  10. Biofuels, poverty, and growth

    DEFF Research Database (Denmark)

    Arndt, Channing; Benfica, Rui; Tarp, Finn;

    2010-01-01

    This paper assesses the implications of large-scale investments in biofuels for growth and income distribution. We find that biofuels investment enhances growth and poverty reduction despite some displacement of food crops by biofuels. Overall, the biofuel investment trajectory analyzed increases...... Mozambique's annual economic growth by 0.6 percentage points and reduces the incidence of poverty by about 6 percentage points over a 12-year phase-in period. Benefits depend on production technology. An outgrower approach to producing biofuels is more pro-poor, due to the greater use of unskilled labor...... and accrual of land rents to smallholders, compared with the more capital-intensive plantation approach. Moreover, the benefits of outgrower schemes are enhanced if they result in technology spillovers to other crops. These results should not be taken as a green light for unrestrained biofuels development...

  11. Biofuel goes underground

    Energy Technology Data Exchange (ETDEWEB)

    Tollinsky, Norm

    2011-09-15

    Kirkland Lake Gold, a gold producer, is switching to a blend of biofuel to power the mine's underground equipment. Kirkland Lake Gold is using a soy-based product which has several advantages: less expensive: for example, the soybean-based biofuel used by Kirkland Lake Gold is 10 cents a liter less expensive than diesel; cleaner: biofuel can reduce emissions by up to 80 per cent compared to conventional diesel; and safer: biofuel is safer than miner's diesel because it has a much higher flash point. Testing with soybean-based biofuel began in the early 90s but its price was too high at that time. The federal government's regulation of renewable fuel quotas has led to the better availability of biofuel now. The supply should be doubled to meet government quotas.

  12. Global Assessments and Guidelines for Sustainable Liquid Biofuel Production in Developing Countries : Final report

    NARCIS (Netherlands)

    Bernd, F.; Reinhardt, G.; Malavelle, J.; Faaij, A.; van Eijck, Janske; van der Hilst, Floortje; Batidzirai, Batidzirai; Fritsche, U.

    2013-01-01

    The Global Environment Facility (GEF) aims to set clear policies and priorities for future work and investments in biofuel related projects and to provide guidance to countries on how to select sustainable biofuel projects. Three UN agencies, UNEP, UNIDO and FAO, in collaboration with three research

  13. [Clinical trials with advanced therapy medicinal products].

    Science.gov (United States)

    Schüssler-Lenz, M; Schneider, C K

    2010-01-01

    For advanced therapies, the same basic principles for assessment apply as for any other biotechnological medicinal product. Nevertheless, the extent of data for quality, safety, and efficacy can be highly specific. Until recently, advanced therapies were not uniformly regulated across Europe, e.g., tissue engineered products were regulated either as medicinal products or medical devices. Thus, for some products no data from clinical studies are available, e.g., for autologous chondrocyte products. The draft guideline on Good Clinical Practice for clinical trials with advanced therapies describes specific additional requirements, e.g., ensuring traceability. Most clinical studies with advanced therapies in Germany are still in early phase I or II trials with highly divergent types of products and clinical indications. The Committee for Advanced Therapies (CAT) at the European Medicines Agency (EMEA) has been established to meet the scientific and regulatory challenges with advanced therapies.

  14. Biofuel production and climate mitigation potential from marginal lands in US North Central region

    Science.gov (United States)

    Gelfand, I.; Sahajpal, R.; Zhang, X.; Izaurralde, R. C.; Robertson, G. P.

    2010-12-01

    An ever-increasing demand for liquid fuels, amidst concerns of anthropogenic impacts on the environment and fossil fuels availability, has spurred a strong interest in the development of agriculturally-based renewable energy sources. However, increasing demand for food as well as direct and indirect effects on land use, have raised concerns about reliance on grain-based ethanol and shifted research towards the direction of cellulosic feedstocks. In order to understand the future possibility for using agricultural systems for bio-fuel production, we present here a full greenhouse gas (GHG) balance of six potential sources of cellulosic feedstocks production. From 1991 to 2008, we measured GHGs sinks and sources in cropped and nearby unmanaged ecosystems in SW Michigan. The measurements included soil fluxes of GHGs (N2O and CH4), soil organic carbon concentration change, agronomic practices data, and biomass yields. We analyzed two types of intensively managed annual cropping systems under corn-soybean-wheat rotation (conventional tillage and no till), two perennial systems (alfalfa and poplar plantation), and one successional system. The use of agricultural residues for biofuel feedstock from conventionally-tilled crops had the lowest climate stabilization potential (-9 ±13 gCO2e m-2 y-1). In contrast, biomass collected from a successional system fertilized with N at123 kg ha-1y-1 showed the highest climate stabilization potential (-749 ±30 gCO2e m-2 y-1). We used our results to parameterize the EPIC model, which, together with GIS analysis was used to scale up the biomass productivity of the best environmentally performing systems to the marginal lands of the 10-state U.S. North Central region. Assuming 80 km as the maximum distance for road haulage to the biorefinery from the field, we identified 32 potential biorefinery placements each capable of supplying sufficient feedstock to produce at least 133 × 106 L y-1. In total, ethanol production from marginal

  15. BioBoost. Biomass based energy intermediates boosting bio-fuel production

    Energy Technology Data Exchange (ETDEWEB)

    Niebel, Andreas [Karlsruher Institut fuer Technologie (KIT), Karlsruhe (Germany). Institut fuer Katalyseforschung und -technologie (IKFT)

    2013-10-01

    To increase the share of biomass for renewable energy in Europe conversion pathways which are economic, flexible in feedstock and energy efficient are needed. The BioBoost project concentrates on dry and wet residual biomass and wastes as feedstock for de-central conversion by fast pyrolysis, catalytic pyrolysis and hydrothermal carbonization to the intermediate energy carriers oil, coal or slurry. Based on straw the energy density increases from 2 to 20-30 GJ/m{sup 3}, enabling central GW scale gasification plants for bio-fuel production. A logistic model for feedstock supply and connection of de-central with central conversion is set up and validated allowing the determination of costs, the number and location of de-central and central sites. Techno/economic and environmental assessment of the value chain supports the optimization of products and processes. The utilization of energy carriers is investigated in existing and coming applications of heat and power production and synthetic fuels and chemicals. (orig.)

  16. Heterologous Expression of Plant Cell Wall Degrading Enzymes for Effective Production of Cellulosic Biofuels

    Directory of Open Access Journals (Sweden)

    Sang-Kyu Jung

    2012-01-01

    Full Text Available A major technical challenge in the cost-effective production of cellulosic biofuel is the need to lower the cost of plant cell wall degrading enzymes (PCDE, which is required for the production of sugars from biomass. Several competitive, low-cost technologies have been developed to produce PCDE in different host organisms such as Escherichia coli, Zymomonas mobilis, and plant. Selection of an ideal host organism is very important, because each host organism has its own unique features. Synthetic biology-aided tools enable heterologous expression of PCDE in recombinant E. coli or Z. mobilis and allow successful consolidated bioprocessing (CBP in these microorganisms. In-planta expression provides an opportunity to simplify the process of enzyme production and plant biomass processing and leads to self-deconstruction of plant cell walls. Although the future of currently available technologies is difficult to predict, a complete and viable platform will most likely be available through the integration of the existing approaches with the development of breakthrough technologies.

  17. Directed evolution of a cellodextrin transporter for improved biofuel production under anaerobic conditions in Saccharomyces cerevisiae.

    Science.gov (United States)

    Lian, Jiazhang; Li, Yanglin; HamediRad, Mohammad; Zhao, Huimin

    2014-08-01

    Introduction of a cellobiose utilization pathway consisting of a cellodextrin transporter and a β-glucosidase into Saccharomyces cerevisiae enables co-fermentation of cellobiose and xylose. Cellodextrin transporter 1 (CDT1) from Neurospora crassa has been established as an effective transporter for the engineered cellobiose utilization pathways. However, cellodextrin transporter 2 (CDT2) from the same species is a facilitator and has the potential to be more efficient than CDT1 under anaerobic conditions due to its energetic benefits. Currently, CDT2 has a very low activity and is considered rate-limiting in cellobiose fermentation. Here, we report the directed evolution of CDT2 with an increased cellobiose uptake activity, which results in improved cellobiose fermentation under anaerobic conditions. After three rounds of directed evolution, the cellobiose uptake activity of CDT2 was increased by 2.2-fold, which resulted from both increased specific activity and transporter expression level. Using high cell density fermentation under anaerobic conditions, the evolved mutant conferred 4.0- and 4.4-fold increase in the cellobiose consumption rate and ethanol productivity, respectively. In addition, although the cellobiose uptake activity was still lower than that of CDT1, the engineered CDT2 showed significantly improved cellobiose consumption and ethanol production under anaerobic conditions, representing the energetic benefits of a sugar facilitator for anaerobic cellobiose fermentation. This study demonstrated that anaerobic biofuel production could be significantly improved via directed evolution of a sugar transporter protein in yeast. PMID:24519319

  18. Energy crops for biofuel production: Analysis of the potential in Tuscany

    Energy Technology Data Exchange (ETDEWEB)

    Marta, A. Dalla; Mancini, M.; Ferrise, R.; Bindi, M.; Orlandini, S. [Department of Plant, Soil and Environmental Science, University of Florence, Piazzale delle Cascine 18 - 50144 Firenze (Italy)

    2010-07-15

    The possibility of using biomass as a source of energy in reducing green-house gas emissions is a matter of great interest. In particular, biomasse from agriculture represent one of the largest and most diversified sources to be exploited and more specifically, ethanol and diesel deriving from biomass have the potential to be a sustainable means of replacing fossil fuels for transportation. Nevertheless, the cultivation of dedicated energy crops does meet with some criticism (competitiveness with food crop cultivation, water requirements, use of fertilizers, etc.) and the economical and environmental advantages of this activity depend on accurate evaluations of the total efficiency of the production system. This paper illustrates the production potential of two energy crops, sunflower (Helianthus annuus) and maize (Zea mais), cultivated with different water and fertilization supplies in the region of Tuscany, in central Italy. A 50-year climatic series of 19 weather stations scattered around Tuscany was used to run the crop model CropSyst for obtaining crop biomass predictions. The effect of climate change and variability was analyzed and the potential production of bioenergy was investigated in terms of pure vegetable oil (sunflower) and bioethanol (maize). The results demonstrated that despite a reduction in crop yields and an increase of their variability due to climate change, the cultivation of maize in the regional set-aside areas would be capable of supplying approximately 50% of the energy requirements in terms of biofuel for transportation obtained, while the cultivation of a sunflower crops would supply less than 10%. (author)

  19. The significance of nitrous oxide emission due to cropping of grain for biofuel production: a Swedish perspective

    Science.gov (United States)

    Kasimir Klemedtsson, Å.; Smith, K. A.

    2011-12-01

    The current regulations governing production of biofuels in the European Union require that they have to mitigate climate change, by producing >35% less greenhouse gases (GHG) than fossil fuels. There is a risk that this may not be achievable, since land use for crop production inevitably emits the potent GHG nitrous oxide (N2O), due to nitrogen fertilisation and cycling in the environment. We analyse first-generation biofuel production on agricultural land and conclude that efficient agricultural crop production resulting in a good harvest and low N2O emission can fulfil the EU standard, and is possible under certain conditions for the Swedish agricultural and bioethanol production systems. However, in years having low crop yields, and where cropping is on organic soils, total GHG emissions per unit of fuel produced can be even higher than those released by burning of fossil fuels. In general, the N2O emission size in Sweden and elsewhere in northern Europe is such that there is a >50% chance that the 35% saving requirement will not be met. Thus ecosystem N2O emissions have to be convincingly assessed. Here we compare Swedish emission data with values estimated by means of statistical models and by a global, top-down, approach; the measurements and the predictions often show higher values that would fail to meet the EU standard and thus prevent biofuel production development.

  20. Assessment of the energy and economic performance of second generation biofuel production processes using energy market scenarios

    International Nuclear Information System (INIS)

    Highlights: ► Consistent evaluation of biofuel processes using future energy market scenarios. ► Scenario-specific evaluation of energetic, economic and environmental performance. ► Biomass-use allocated with CO2 emissions based on reference biomass fuel user. ► Assumed CO2-neutrality of biomass overestimates the real-life GHG emission reductions. ► Synthetic natural gas production from biomass needs policy support to be economically viable. -- Abstract: In this paper performance assessment of second generation biofuel production using energy market scenarios and system-level performance indicators is proposed. During biofuel production a number of products and services can be co-generated while import of energy services (e.g. electricity and heat) in addition to the fuel supply may also be needed. This needs to be reflected by a well-defined performance indicator enabling a comparison between different process alternatives. A marginal production perspective is proposed in this study for the definition of a general energy performance indicator, recalculating all services to primary energy on a system level. The Energy Price and Carbon Balance Scenarios (ENPAC) tool developed at Chalmers is used for the definition of the energy system background. Thereby, a scenario-specific comparison of the processes’ thermodynamic, economic and carbon footprint performance is possible. The usefulness of the approach is illustrated for production of synthetic natural gas (SNG) from biomass. The shortcomings of common performance indicators are also discussed.

  1. Algae as a Feedstock for Biofuels. An Assessment of the Current Status and Potential for Algal Biofuels Production. Joint Summary report of IEA-AMF Annex XXXIV-2 and IEA Bioenergy Task 39

    Energy Technology Data Exchange (ETDEWEB)

    O' Conner, D. [S and T2 Consultants, Inc. (Canada)

    2011-09-15

    In 2010, the IEA Advanced Motor Fuels Implementing Agreement and the IEA Bioenergy Task 39 both commissioned reports on the status and potential opportunities for Algal Biofuels. While there were substantial similarities in the findings of the two reports, each report provides unique perspectives on different aspects of the technology and the opportunities. This summary draws on both of those reports. The Task 39 report (Bioenergy Algal Biofuels.pdf) was authored by Al Darzins and Philip Pienkos (NREL, US) and Les Edye (BioIndustry Partners, Australia). The IEA AMF report was prepared by Karen Sikes and Ralph McGill (Sentech, Inc. US) and Martijn Van Walwijk (Independent Researcher).

  2. Life cycle assessment of energy products: environmental impact assessment of biofuels; Oekobilanz von Energieprodukten: Oekologische Bewertung von Biotreibstoffen

    Energy Technology Data Exchange (ETDEWEB)

    Zah, R.; Boeni, H.; Gauch, M.; Hischier, R.; Lehmann, M.; Waeger, P.

    2007-05-15

    This final report for the Swiss Federal Office of Energy (SFOE) deals with the results of a study that evaluated the environmental impact of the entire production chain of fuels made from biomass and used in Switzerland. Firstly, the study supplies an analysis of the possible environmental impacts of biofuels that can be used as a basis for political decisions. Secondly, an environmental life cycle assessment (LCA) of various biofuels is presented. In addition, the impacts of fuel use are compared with other uses for bioenergy such as the generation of electricity and heat. The methods used in the LCA are discussed, including the Swiss method of ecological scarcity (Environmental Impact Points, UBP 06), and the European Eco-indicator 99 method. The results of the study are discussed, including the finding that not all biofuels can reduce environmental impacts as compared to fossil fuels. The role to be played by biofuels produced in an environmentally-friendly way together with other forms of renewable energy in our future energy supply is discussed.

  3. Effects of Environmental Factors and Nutrient Availability on the Biochemical Composition of Algae for Biofuels Production: A Review

    Directory of Open Access Journals (Sweden)

    Ganti S. Murthy

    2013-09-01

    Full Text Available Due to significant lipid and carbohydrate production as well as other useful properties such as high production of useful biomolecular substrates (e.g., lipids and the ability to grow using non-potable water sources, algae are being explored as a potential high-yield feedstock for biofuels production. In both natural and engineered systems, algae can be exposed to a variety of environmental conditions that affect growth rate and cellular composition. With respect to the latter, the amount of carbon fixed in lipids and carbohydrates (e.g., starch is highly influenced by environmental factors and nutrient availability. Understanding synergistic interactions between multiple environmental variables and nutritional factors is required to develop sustainable high productivity bioalgae systems, which are essential for commercial biofuel production. This article reviews the effects of environmental factors (i.e., temperature, light and pH and nutrient availability (e.g., carbon, nitrogen, phosphorus, potassium, and trace metals as well as cross-interactions on the biochemical composition of algae with a special focus on carbon fixation and partitioning of carbon from a biofuels perspective.

  4. Ionizing Radiation Conversion of Lignocellulosic Biomass from Sugarcane Bagasse to Production Ethanol Biofuel

    International Nuclear Information System (INIS)

    Sugarcane bagasse has been considered as a substrate for single cell protein, animal feed, and renewable energy production. Sugarcane bagasse generally contain up to 45% glucose polymer cellulose, 40% hemicelluloses, and 20% lignin. Pure cellulose is readily depolymerised by radiation, but in biomass, the cellulose is intimately bonded with lignin, that protect it from radiation effects. The objective of this study is the evaluation of the electron beam irradiation as a pre-treatment to enzymatic hydrolysis of cellulose in order to facilitate its fermentation and improves the production of ethanol biofuel. Samples of sugarcane bagasse were obtained in sugar/ethanol Iracema Mill sited in Piracicaba, Brazil, and were irradiated using Radiation Dynamics Electron Beam Accelerator with 1.5 MeV energy and 37kW, in batch systems. The applied absorbed doses of the fist sampling, Bagasse A, were 20 kGy, 50 kGy, 100 kGy and 200 kGy. After the evaluation the preliminary obtained results, it was applied lower absorbed doses in the second assay: 5 kGy, 10 kGy, 20 kGy, 30 kGy, 50 kGy, 70 kGy, 100 kGy and 150 kGy. The electron beam processing took to changes in the sugarcane bagasse structure and composition, lignin and cellulose cleavage. The yield of enzymatic hydrolyzes of cellulose increase about 75 % with 30 kGy of absorbed dose. (author)

  5. N2O release from agro-biofuel production negates global warming reduction by replacing fossil fuels

    Directory of Open Access Journals (Sweden)

    A. R. Mosier

    2007-08-01

    Full Text Available The relationship, on a global basis, between the amount of N fixed by chemical, biological or atmospheric processes entering the terrestrial biosphere, and the total emission of nitrous oxide (N2O, has been re-examined, using known global atmospheric removal rates and concentration growth of N2O as a proxy for overall emissions. The relationship, in both the pre-industrial period and in recent times, after taking into account the large-scale changes in synthetic N fertiliser production and deforestation, is consistent, showing an overall conversion factor of 3–5%. This factor is covered only in part by the ~1% of "direct" emissions from agricultural crop lands estimated by IPCC (2006, or the "indirect" emissions cited therein. This means that the extra N2O entering the atmosphere as a result of using N to produce crops for biofuels will also be correspondingly greater than that estimated just on the basis of IPCC (2006. When the extra N2O emission from biofuel production is calculated in "CO2-equivalent" global warming terms, and compared with the quasi-cooling effect of "saving" emissions of fossil fuel derived CO2, the outcome is that the production of commonly used biofuels, such as biodiesel from rapeseed and bioethanol from corn (maize, can contribute as much or more to global warming by N2O emissions than cooling by fossil fuel savings. Crops with less N demand, such as grasses and woody coppice species have more favourable climate impacts. This analysis only considers the conversion of biomass to biofuel. It does not take into account the use of fossil fuel on the farms and for fertilizer and pesticide production, but it also neglects the production of useful co-products. Both factors partially compensate each other. This needs to be analyzed in a full life cycle assessment.

  6. Biofuel Production in Ireland—An Approach to 2020 Targets with a Focus on Algal Biomass

    OpenAIRE

    Fionnuala Murphy; Ger Devlin; Rory Deverell; Kevin McDonnell

    2013-01-01

    Under the Biofuels Obligation Scheme in Ireland, the biofuels penetration rate target for 2013 was set at 6% by volume from a previous 4% from 2010. In 2012 the fuel blend reached 3%, with approximately 70 million L of biodiesel and 56 million L of ethanol blended with diesel and gasoline, respectively. Up to and including April 2013, the current blend rate in Ireland for biodiesel was 2.3% and for bioethanol was 3.7% which equates to approximately 37.5 million L of biofuel for the first four...

  7. Novel biofuel formulations for enhanced vehicle performance

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Dennis [Michigan State Univ., East Lansing, MI (United States); Narayan, Ramani [Michigan State Univ., East Lansing, MI (United States); Berglund, Kris [Michigan State Univ., East Lansing, MI (United States); Lira, Carl [Michigan State Univ., East Lansing, MI (United States); Schock, Harold [Michigan State Univ., East Lansing, MI (United States); Jaberi, Farhad [Michigan State Univ., East Lansing, MI (United States); Lee, Tonghun [Michigan State Univ., East Lansing, MI (United States); Anderson, James [Michigan State Univ., East Lansing, MI (United States); Wallington, Timothy [Michigan State Univ., East Lansing, MI (United States); Kurtz, Eric [Michigan State Univ., East Lansing, MI (United States); Ruona, Will; Hass, Heinz

    2013-08-30

    engine under highly instrumented conditions. Simulation of and experimentation on combustion in single and multicylinder engines was carried out in detail throughout the project. The combustion behavior of biofuel blends neat and in petroleum were characterized in the MSU optical engine, in part to validate results obtained in the RCM and to provide data for comparison with simulations. Simulation of in- cylinder, low-temperature combustion included development of an extensive fuel injection model that included fuel spray breakup, evaporation, and ignition, along with prediction of cylinder temperature, pressure, and work produced. Single cylinder and multicylinder engine tests under advanced low-temperature combustion conditions conducted at Ford Motor Company validated experimental and simulation results obtained in the MSU engine and in MSU simulations. Single cylinder engine tests of an advanced biofuel containing biodiesel and dibutyl succinate, carried out under low-temperature combustion conditions, showed similar power generation and gas-phase emissions (CO, HC, NOx), but a reduction in particulates of as much as 60% relative to neat biodiesel and 95% relative to petroleum diesel at the same operating conditions. This remarkable finding suggests that biofuels may be able to play a role in eliminating the need for particulate removal systems in diesel vehicles. The multicylinder engine tests at Ford, carried out using butyl nonanoate as an advanced biofuel, also gave promising results, showing a strong decline in particulate emissions and simultaneously a modest decrease in NOx emissions relative to standard petroleum diesel at the same conditions. In summary, this project has shown that advanced biofuels and their blends are capable of maintaining performance while reducing emissions, particularly particulates (soot), in 3 compression ignition engines. The interdisciplinary nature of biofuel production and testing has identified fuel properties that are capable

  8. Biofuels and sustainability.

    Science.gov (United States)

    Solomon, Barry D

    2010-01-01

    Interest in liquid biofuels production and use has increased worldwide as part of government policies to address the growing scarcity and riskiness of petroleum use, and, at least in theory, to help mitigate adverse global climate change. The existing biofuels markets are dominated by U.S. ethanol production based on cornstarch, Brazilian ethanol production based on sugarcane, and European biodiesel production based on rapeseed oil. Other promising efforts have included programs to shift toward the production and use of biofuels based on residues and waste materials from the agricultural and forestry sectors, and perennial grasses, such as switchgrass and miscanthus--so-called cellulosic ethanol. This article reviews these efforts and the recent literature in the context of ecological economics and sustainability science. Several common dimensions for sustainable biofuels are discussed: scale (resource assessment, land availability, and land use practices); efficiency (economic and energy); equity (geographic distribution of resources and the "food versus fuel" debate); socio-economic issues; and environmental effects and emissions. Recent proposals have been made for the development of sustainable biofuels criteria, culminating in standards released in Sweden in 2008 and a draft report from the international Roundtable on Sustainable Biofuels. These criteria hold promise for accelerating a shift away from unsustainable biofuels based on grain, such as corn, and toward possible sustainable feedstock and production practices that may be able to meet a variety of social, economic, and environmental sustainability criteria. PMID:20146765

  9. Positive aspects issued from bio-corrosion studies: from hydrogen production to biofuel cells

    International Nuclear Information System (INIS)

    Microbially influenced corrosion or bio-corrosion is a problem that generates heavy global economic losses (several billion euros per year). In spite of the progress made on the understanding of the underlying mechanisms, the complexity of the phenomenon has prevented finding definitive solutions to the problem and continues to inspire many research works. The participation in bio-corrosion of catalytic mechanisms induced by weak acids was studied in this work. Another objective of the thesis has been to take advantage from catalytic phenomena found in bio corrosion research to apply them in other areas: energy production with biofuel cells or electrochemical hydrogen production in mild conditions. This work has shown that the presence of weak acids and amino acids inside bio films could play a major role in steel bio-corrosion accelerating the phenomenon through the catalysis of the water reduction reaction. The reversibility of this mechanism, discerned and proved here, could explain the corrosion increase when hydrogen is removed (bacterial consumption, agitation...). In addition, phosphates allow the production of hydrogen by electrolysis in mild ph conditions (pH 4.0 - 8.0) with an equal or better performance than those found in alkaline electrolysis. Finally, industrial materials like stainless steel and titanium could be used in the fabrication of enzymatic electrodes for biosensors or microsystems. The use of the glucose oxidase / glucose system in an aqueous fuel cell with a stainless steel cathode, allows the improvement of the cell performance thanks to the production of hydrogen peroxide that is easily reduced. Moreover, the use of materials with micro-structured surfaces like sandblasted steels deserve to be studied in detail to exploit the remarkable reactivity they present compared to smooth electrodes. (author)

  10. Positive aspects issued from bio corrosion studies: from hydrogen production to biofuel cells

    International Nuclear Information System (INIS)

    Microbially influenced corrosion or bio corrosion is a problem that generates heavy global economic losses (several billion euros per year). In spite of the progress made on the understanding of the underlying mechanisms, the complexity of the phenomenon has prevented finding definitive solutions to the problem and continues to inspire many research works. The participation in bio corrosion of catalytic mechanisms induced by weak acids was studied in this work. Another objective of the thesis has been to take advantage from catalytic phenomena found in bio corrosion research to apply them in other areas: energy production with biofuel cells or electrochemical hydrogen production in mild conditions. This work has shown that the presence of weak acids and amino acids inside bio-films could play a major role in steel bio corrosion accelerating the phenomenon through the catalysis of the water reduction reaction. The reversibility of this mechanism, discerned and proved here, could explain the corrosion increase when hydrogen is removed (bacterial consumption, agitation...). In addition, phosphates allow the production of hydrogen by electrolysis in mild pH conditions (pH 4.0 - 8.0) with an equal or better performance than those found in alkaline electrolysis. Finally, industrial materials like stainless steel and titanium could be used in the fabrication of enzymatic electrodes for biosensors or microsystems. The use of the glucose oxidase/glucose system in an aqueous fuel cell with a stainless steel cathode, allows the improvement of the cell performance thanks to the production of hydrogen peroxide that is easily reduced. Moreover, the use of materials with micro-structured surfaces like sandblasted steels deserve to be studied in detail to exploit the remarkable reactivity they present compared to smooth electrodes. (author)

  11. Economics and Uncertainty of Lignocellulosic Biofuel Production from Energy Cane and Sweet Sorghum in South Texas

    OpenAIRE

    Monge, Juan J.; Ribera, Luis A.; Jifon, John L.; Silva, Jorge A. da; Richardson, James W.

    2014-01-01

    Government support uncertainty, scarce yiel d information, and the inherent risk in bio- economic phenomena are some of the deterrents faced by investors in the nascent cellulosic biofuel industry. A financial probabilistic model was developed to contrast the economic feasibility of producing cellulosic biofuels from energy cane and sweet sorghum using three technologies: hydrolysis, pyrolysis, and gasification. Hydrolysis and pyrolysis proved feasible (showed possibilities of a positive net ...

  12. Engineering microbial biofuel tolerance and export using efflux pumps.

    Science.gov (United States)

    Dunlop, Mary J; Dossani, Zain Y; Szmidt, Heather L; Chu, Hou Cheng; Lee, Taek Soon; Keasling, Jay D; Hadi, Masood Z; Mukhopadhyay, Aindrila

    2011-05-10

    Many compounds being considered as candidates for advanced biofuels are toxic to microorganisms. This introduces an undesirable trade-off when engineering metabolic pathways for biofuel production because the engineered microbes must balance production against survival. Cellular export systems, such as efflux pumps, provide a direct mechanism for reducing biofuel toxicity. To identify novel biofuel pumps, we used bioinformatics to generate a list of all efflux pumps from sequenced bacterial genomes and prioritized a subset of targets for cloning. The resulting library of 43 pumps was heterologously expressed in Escherichia coli, where we tested it against seven representative biofuels. By using a competitive growth assay, we efficiently distinguished pumps that improved survival. For two of the fuels (n-butanol and isopentanol), none of the pumps improved tolerance. For all other fuels, we identified pumps that restored growth in the presence of biofuel. We then tested a beneficial pump directly in a production strain and demonstrated that it improved biofuel yields. Our findings introduce new tools for engineering production strains and utilize the increasingly large database of sequenced genomes.

  13. Current market of industrial bio-products and biofuels, and predictable evolutions by 2015/2030. Synthesis

    International Nuclear Information System (INIS)

    The main objectives of this study were to describe the current status of the energetic and industrial bio-product markets (biofuels, bio-lubricants, biomaterials, papers, cosmetics, and so on), to identify and analyze the evolution perspectives of these new markets on a long and medium term, to define scenarios of evolution for different sectors (agro-industry, energy, organic chemistry), to identify the most promising new markets, and to select the priority agro-industrial sectors

  14. The Selective Use of Hypochlorite to Prevent Pond Crashes for Algae-Biofuel Production.

    Science.gov (United States)

    Park, Sichoon; Van Ginkel, Steven W; Pradeep, Priya; Igou, Thomas; Yi, Christine; Snell, Terry; Chen, Yongsheng

    2016-01-01

    Although algae-biofuels have many advantages including high areal productivity, algae can be preyed upon by amoebas, protozoans, ciliates, and rotifers, particularly in open pond systems. Thus, these higher organisms need to be controlled. In this study, Chlorella kessleri was used as the algal culture and Brachionus calyciflorus as the source of predation. The effect of sodium hypochlorite (bleach) was tested with the goal of totally inhibiting the rotifer while causing minor inhibition to the alga. The 24-hr LC(50) for B. calyciflorus in spring water was 0.198 mg Cl/L while the 24-hr LC(50) for C. kessleri was 0.321 mg Cl/L. However, chlorine dissipates rapidly as the algae serves as reductant. Results showed a chlorine dosage between 0.45 to 0.6 mg Cl/L and a dosing interval of two hours created the necessary chlorine concentrations to inhibit predation while letting the algae grow; thus giving algae farmers a tool to prevent pond crashes.

  15. Recent progress and future challenges in algal biofuel production [version 1; referees: 4 approved

    Directory of Open Access Journals (Sweden)

    Jonathan B. Shurin

    2016-10-01

    Full Text Available Modern society is fueled by fossil energy produced millions of years ago by photosynthetic organisms. Cultivating contemporary photosynthetic producers to generate energy and capture carbon from the atmosphere is one potential approach to sustaining society without disrupting the climate. Algae, photosynthetic aquatic microorganisms, are the fastest growing primary producers in the world and can therefore produce more energy with less land, water, and nutrients than terrestrial plant crops. We review recent progress and challenges in developing bioenergy technology based on algae. A variety of high-value products in addition to biofuels can be harvested from algal biomass, and these may be key to developing algal biotechnology and realizing the commercial potential of these organisms. Aspects of algal biology that differentiate them from plants demand an integrative approach based on genetics, cell biology, ecology, and evolution. We call for a systems approach to research on algal biotechnology rooted in understanding their biology, from the level of genes to ecosystems, and integrating perspectives from physical, chemical, and social sciences to solve one of the most critical outstanding technological problems.

  16. Logging residues from regeneration fellings for biofuel production - a GIS-based availability analysis in Finland

    Energy Technology Data Exchange (ETDEWEB)

    Ranta, Tapio [Department of Energy and Environmental Technology, Mikkeli (Finland)

    2005-02-01

    Finland has large forest fuel resources. However, the use of forest fuels for energy production has been low, except for small-scale use in heating. According to national action plans and programs related to promotion of biofuels, the utilization of such resources will be multiplied over the next few years. The most significant part of this growth will be based on the utilization of forest fuels, produced from logging residues of regeneration fellings, in industrial and municipal power and heating plants. Availability of logging residues was analyzed by means of resource and demand approach in order to identify the most suitable regions with focus on increasing the forest fuel usage. The analysis included availability comparisons between power plant sites and resource allocation in a least-cost manner according to supply cost, and between a predefined power plant structure under given demand and supply constraints. Spatial analysis of worksite factors and regional geographies was carried out using the Graphics Information System-model (GIS-model) environment via geoprocessing and cartographic modeling tools. According to the site-specific analysis, the supply potential of different locations can differ multifold. However, due to technical and economical reasons of the fuel supply and dense power plant infrastructure, the supply potential is limited to plant level. Therefore, the potential and supply-cost calculations depend on site-specific matters, where regional characteristics of resources and infrastructure should be taken into consideration, eg, by using a GIS-modelling approach constructed in this study. (Author)

  17. Strategies for the production of cell wall-deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production.

    Science.gov (United States)

    Park, Sang-Hyuck; Ong, Rebecca Garlock; Sticklen, Mariam

    2016-06-01

    Microbial cell wall-deconstructing enzymes are widely used in the food, wine, pulp and paper, textile, and detergent industries and will be heavily utilized by cellulosic biorefineries in the production of fuels and chemicals. Due to their ability to use freely available solar energy, genetically engineered bioenergy crops provide an attractive alternative to microbial bioreactors for the production of cell wall-deconstructing enzymes. This review article summarizes the efforts made within the last decade on the production of cell wall-deconstructing enzymes in planta for use in the deconstruction of lignocellulosic biomass. A number of strategies have been employed to increase enzyme yields and limit negative impacts on plant growth and development including targeting heterologous enzymes into specific subcellular compartments using signal peptides, using tissue-specific or inducible promoters to limit the expression of enzymes to certain portions of the plant or certain times, and fusion of amplification sequences upstream of the coding region to enhance expression. We also summarize methods that have been used to access and maintain activity of plant-generated enzymes when used in conjunction with thermochemical pretreatments for the production of lignocellulosic biofuels. PMID:26627868

  18. Methods of dealing with co-products of biofuels in life-cycle analysis and consequent results within the U.S. context

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Michael, E-mail: mqwang@anl.gov [Center for Transportation Research, Argonne National Laboratory, Argonne, IL 60439 (United States); Huo Hong [Institute of Energy, Environment, and Economics, Tsinghua University, Beijing, 100084 (China); Arora, Salil [Center for Transportation Research, Argonne National Laboratory, Argonne, IL 60439 (United States)

    2011-10-15

    Products other than biofuels are produced in biofuel plants. For example, corn ethanol plants produce distillers' grains and solubles. Soybean crushing plants produce soy meal and soy oil, which is used for biodiesel production. Electricity is generated in sugarcane ethanol plants both for internal consumption and export to the electric grid. Future cellulosic ethanol plants could be designed to co-produce electricity with ethanol. It is important to take co-products into account in the life-cycle analysis of biofuels and several methods are available to do so. Although the International Standard Organization's ISO 14040 advocates the system boundary expansion method (also known as the 'displacement method' or the 'substitution method') for life-cycle analyses, application of the method has been limited because of the difficulty in identifying and quantifying potential products to be displaced by biofuel co-products. As a result, some LCA studies and policy-making processes have considered alternative methods. In this paper, we examine the available methods to deal with biofuel co-products, explore the strengths and weaknesses of each method, and present biofuel LCA results with different co-product methods within the U.S. context.

  19. Identification and quantification of indirect land and resource use changes – Challenges caused by expanding liquid biofuel production

    OpenAIRE

    Manninen, Kaisa; Antikainen, Riina; Soimakallio, Sampo; Simola, Antti; Thun, Rabbe

    2012-01-01

    The indirect effects of bioenergy and transport biofuels with a special focus on indirect land use changes (ILUC) and indirect impacts on resource use was studied. Three case studies were examined. First, a literature review of Brazilian sugarcane ethanol production is presented. Second, it was assessed how the increase in liquid biofuel production alters the allocation of land use patterns and other sources of non-CO2 greenhouse gas emissions in Finland by using general equilibrium (CGE) mod...

  20. Effect of pre-treatments on the production of biofuels from Phaeodactylum tricornutum.

    Science.gov (United States)

    Caporgno, M P; Olkiewicz, M; Torras, C; Salvadó, J; Clavero, E; Bengoa, C

    2016-07-15

    Several characteristics make Phaeodactylum tricornutum potential candidate for biofuels production such as methane and biodiesel. For this reason, some alternatives are evaluated in this manuscript to improve the conversion of this microalgae into methane. One of these alternatives is the addition of sewage sludge to Phaeodactylum tricornutum for anaerobic co-digestion. Although the co-digestion resulted in lack of synergy, the absence of inhibition indicated that both substrates could be co-digested under certain circumstances, for example if microalgae are cultivated for wastewater treatment purposes. The extraction of lipids using organic solvents has been evaluated for biodiesel production but also as a pre-treatment for anaerobic digestion. The results revealed that the type of solvent influences lipid and biodiesel yields. The high polarity of the mixture methanol/hexane increased the lipid and the biodiesel yields from 10 ± 1 to 53 ± 2 gLipids/100 gVS and from 7 ± 1 to 11 ± 1 gBiodiesel/100 gVS compared with hexane. However, none of these solvents affected the composition of biodiesel. Regarding the methane production after the extraction, it yielded 257 ± 8 and 180 ± 6 mLCH4/gVS from lipid-extracted P. tricornutum using hexane and methanol/hexane respectively. The methane production from the raw microalga was 258 ± 5 mLCH4/gVS in the same experiment. The difference in methane production, mainly after the extraction with methanol/hexane, was a consequence of the changes in the composition of the microalgae after extraction. The extraction did not influence the biodegradability. The ultrasonic pre-treatment prior anaerobic digestion completely disrupted the microalgae cells, but the solubilisation of the organic fraction was scarce (<9.5%). The methane production from pre-treated samples was barely 10-11% higher than the obtained from non pre-treated samples, indicating that the refractory nature of the organic fraction in P

  1. Energy production from burning of mixtures of source-sorted waste fractions and biofuels. Final report; Sameldning av biobraenslen med kaellsorterade avfallsfraktioner. Slutrapport

    Energy Technology Data Exchange (ETDEWEB)

    Olsson, Rolf; Marklund, Stellan; Nilsson, Calle; Burvall, Jan; Hedman, Bjoern [Swedish Univ. of Agricultural Sciences, Umeaa (Sweden). Dept. of Agricultural Research for Northern Sweden

    2002-02-01

    Energy production trough co-combustion of biofuel and the dry fraction of household waste might be an alternative in the future for rural district areas since the present system for recovery has been criticized. So far the results from the co-combustion of briquettes made by energy grass, Reed Canary grass, in mixture with very well separated dry fraction of household waste shows that new developed commercial boiler technology, 100-1000 kW, have been well designed for god function, high efficiency and low emissions using ordinary cyclone flue gas cleaning. By consideration of the new EU incineration waste directive for cocombustion of biofuel and waste fractions the achieved emission levels from this trials in many cases seam to fulfill the directive including critical parameters such as CO and dioxins without high advanced flue gas cleaning. Mixture of waste together with energy grass does not raise the emission levels of HCl compared to pure energy grass combustion. The importance of developing systems for well sorted dry fraction of household waste have been verified in the project. The quality insurance of the fuel is necessary to consider the new EU directive. In this project studies of systems for sorting of dry waste have been compared. A system in which the sorting part can be identified and attained by information and premium awards shows obvious advantages compared with local central placed container systems for the dry waste.

  2. Agrigenomics for microalgal biofuel production: an overview of various bioinformatics resources and recent studies to link OMICS to bioenergy and bioeconomy.

    Science.gov (United States)

    Misra, Namrata; Panda, Prasanna Kumar; Parida, Bikram Kumar

    2013-11-01

    Microalgal biofuels offer great promise in contributing to the growing global demand for alternative sources of renewable energy. However, to make algae-based fuels cost competitive with petroleum, lipid production capabilities of microalgae need to improve substantially. Recent progress in algal genomics, in conjunction with other "omic" approaches, has accelerated the ability to identify metabolic pathways and genes that are potential targets in the development of genetically engineered microalgal strains with optimum lipid content. In this review, we summarize the current bioeconomic status of global biofuel feedstocks with particular reference to the role of "omics" in optimizing sustainable biofuel production. We also provide an overview of the various databases and bioinformatics resources available to gain a more complete understanding of lipid metabolism across algal species, along with the recent contributions of "omic" approaches in the metabolic pathway studies for microalgal biofuel production.

  3. Agrigenomics for microalgal biofuel production: an overview of various bioinformatics resources and recent studies to link OMICS to bioenergy and bioeconomy.

    Science.gov (United States)

    Misra, Namrata; Panda, Prasanna Kumar; Parida, Bikram Kumar

    2013-11-01

    Microalgal biofuels offer great promise in contributing to the growing global demand for alternative sources of renewable energy. However, to make algae-based fuels cost competitive with petroleum, lipid production capabilities of microalgae need to improve substantially. Recent progress in algal genomics, in conjunction with other "omic" approaches, has accelerated the ability to identify metabolic pathways and genes that are potential targets in the development of genetically engineered microalgal strains with optimum lipid content. In this review, we summarize the current bioeconomic status of global biofuel feedstocks with particular reference to the role of "omics" in optimizing sustainable biofuel production. We also provide an overview of the various databases and bioinformatics resources available to gain a more complete understanding of lipid metabolism across algal species, along with the recent contributions of "omic" approaches in the metabolic pathway studies for microalgal biofuel production. PMID:24044362

  4. Strategy for research and development connected to production o fliquid biofuels; Strategi for forskning og udvikling vedr. fremstilling af flydende biobraendstoffer

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-06-01

    In many large industrialised countries it is expected that engine fuels manufactured from biomass will play an increasing role, especially considering security of supply, reduction of CO{sub 2} emission and increasing rate of employment within farming. The price of biofuel will, however, even in the long term, depending on the biomass and the technology used for production, easily remain significantly higher than the price of the fossil fuel, which biofuel substitutes. The most relevant biofuels today are ethanol and RME (rape seed methyl ester - bio diesel). As regards new technology for ethanol production, a significant knowledge base has been built in Denmark in research collaboration between DTU-BioCentrum and Risoe National Laboratory. The Danish research efforts should primarily focus on development of technology and know-how that can be patented and commercialised internationally and furthermore can be used for establishing a biofuel production in Denmark. (BA)

  5. Study of by-products of agro-food industries which could be used for bio-fuel production (animal fat, used food oils, and wine production by-products). Synthesis of the final report

    International Nuclear Information System (INIS)

    As the Renewable Energy directive proposes the implementation of incentive arrangements for the production of bio-fuels from biomass, this report proposes a synthesis of a study which addressed three by-products of agro-food industry and of catering (collective, traditional, fast) which can help to reach objectives of energy production from biomass: used food oils, rendered animal fat of category 1 and 2, and vinification by-products (grape marc, lees, sludge). The objectives were to quantify, at the French national and regional levels, present resources and uses for these three by-products, non-valorised volumes and thus potentially available volumes for the production of liquid bio-fuels, to identify present actors and their interactions, and to study the potential of local production of liquid bio-fuels. The study comprised a comprehensive analysis of production and valorisation sectors for the three addressed types of by-products, and an identification of recent experiments implemented for the production of liquid bio-fuels. This synthesis states the lessons learned from the study of these three different sectors, and proposes recommendations for further developments

  6. Predicting potential global distributions of two Miscanthus grasses: implications for horticulture, biofuel production, and biological invasions.

    Directory of Open Access Journals (Sweden)

    Heather A Hager

    Full Text Available In many regions, large proportions of the naturalized and invasive non-native floras were originally introduced deliberately by humans. Pest risk assessments are now used in many jurisdictions to regulate the importation of species and usually include an estimation of the potential distribution in the import area. Two species of Asian grass (Miscanthus sacchariflorus and M. sinensis that were originally introduced to North America as ornamental plants have since escaped cultivation. These species and their hybrid offspring are now receiving attention for large-scale production as biofuel crops in North America and elsewhere. We evaluated their potential global climate suitability for cultivation and potential invasion using the niche model CLIMEX and evaluated the models' sensitivity to the parameter values. We then compared the sensitivity of projections of future climatically suitable area under two climate models and two emissions scenarios. The models indicate that the species have been introduced to most of the potential global climatically suitable areas in the northern but not the southern hemisphere. The more narrowly distributed species (M. sacchariflorus is more sensitive to changes in model parameters, which could have implications for modelling species of conservation concern. Climate projections indicate likely contractions in potential range in the south, but expansions in the north, particularly in introduced areas where biomass production trials are under way. Climate sensitivity analysis shows that projections differ more between the selected climate change models than between the selected emissions scenarios. Local-scale assessments are required to overlay suitable habitat with climate projections to estimate areas of cultivation potential and invasion risk.

  7. Spatial Optimization of Cropping Pattern in an Agricultural Watershed for Food and Biofuel Production with Minimum Downstream Pollution

    Science.gov (United States)

    Pv, F.; Sudheer, K.; Chaubey, I.; RAJ, C.; Her, Y.

    2013-05-01

    Biofuel is considered to be a viable alternative to meet the increasing fuel demand, and therefore many countries are promoting agricultural activities that help increase production of raw material for biofuel production. Mostly, the biofuel is produced from grain based crops such as Corn, and it apparently create a shortage in food grains. Consequently, there have been regulations to limit the ethanol production from grains, and to use cellulosic crops as raw material for biofuel production. However, cultivation of such cellulosic crops may have different effects on water quality in the watershed. Corn stover, one of the potential cellulosic materials, when removed from the agricultural field for biofuel production, causes a decrease in the organic nutrients in the field. This results in increased use of pesticides and fertilizers which in turn affect the downstream water quality due to leaching of the chemicals. On the contrary, planting less fertilizer-intensive cellulosic crops, like Switch Grass and Miscanthus, is expected to reduce the pollutant loadings from the watershed. Therefore, an ecologically viable land use scenario would be a mixed cropping of grain crops and cellulosic crops, that meet the demand for food and biofuel without compromising on the downstream water quality. Such cropping pattern can be arrived through a simulation-optimization framework. Mathematical models can be employed to evaluate various management scenarios related to crop production and to assess its impact on water quality. Soil and Water Assessment Tool (SWAT) model is one of the most widely used models in this context. SWAT can simulate the water and nutrient cycles, and also quantify the long-term impacts of land management practices, in a watershed. This model can therefore help take decisions regarding the type of cropping and management practices to be adopted in the watershed such that the water quality in the rivers is maintained at acceptable level. In this study, it

  8. Synthetic biology advances for pharmaceutical production

    OpenAIRE

    Breitling, Rainer; Takano, Eriko

    2015-01-01

    Synthetic biology enables a new generation of microbial engineering for the biotechnological production of pharmaceuticals and other high-value chemicals. This review presents an overview of recent advances in the field, describing new computational and experimental tools for the discovery, optimization and production of bioactive molecules, and outlining progress towards the application of these tools to pharmaceutical production systems.

  9. Biofuels combustion.

    Science.gov (United States)

    Westbrook, Charles K

    2013-01-01

    This review describes major features of current research in renewable fuels derived from plants and from fatty acids. Recent and ongoing fundamental studies of biofuel molecular structure, oxidation reactions, and biofuel chemical properties are reviewed, in addition to combustion applications of biofuels in the major types of engines in which biofuels are used. Biofuels and their combustion are compared with combustion features of conventional petroleum-based fuels. Two main classes of biofuels are described, those consisting of small, primarily alcohol, fuels (particularly ethanol, n-butanol, and iso-pentanol) that are used primarily to replace or supplement gasoline and those derived from fatty acids and used primarily to replace or supplement conventional diesel fuels. Research efforts on so-called second- and third-generation biofuels are discussed briefly.

  10. Investigating and modeling the pyrolysis kinetic of leaves and stems of pistachio trees for biofuel production

    Directory of Open Access Journals (Sweden)

    M Ostad Hoseini

    2016-09-01

    Full Text Available Introduction The lignocelluloses materials have high potential for producing various types of biofuels. These materials include various parts of plants, especially leaves and stems that are left without a specific usage after annual pruning. These residues can be used through slow or fast pyrolysis process for production of liquid and gaseous biofuels. The slow pyrolysis is taking place at temperatures below 500°C while fast pyrolysis process takes place at a temperature above 700°C. Various studies on production of biofuels from plant residues have shown that the temperature, heating rate and the resident time of pyrolysis process are the main factors that affect the final product quality. At present time, in Iran, there are more than 360 thousands hectares of pistachio growing fields which annually produce over 215 thousands metric tons residues which are mainly leaves and stems. The main objective of this study was to measure the heating properties of the powders prepared from the leaves and the stem of pistachio trees. These properties include higher heating value (HHV, lower heating value (LHV and thermal gravimetric analysis (TGA of the powders. Then the powders were separately pyrolysed and the kinetic of the pyrolysis process for producing charcoal from them was investigated. Materials and Methods In this research, leaves and stems of pistachio trees were initially analyzed to determine their chemical constituents including moisture content, volatile compounds, carbon (C, hydrogen (H, nitrogen (N, sulfur (S and oxygen (O content. Using these constituents the height heating value and low heating value for the leaves and the stems were determined. The thermal gravimetric analysis (TGA of the powders was made to select a proper heating temperature for pyrolysis of the powders. In each experiment about 10 g of powder powders were pyrolyzed to produce char. Based on TGA results, the pyrolysis experiments were performed at 350, 400, 450 and

  11. Design and development of polyamine polymer for harvesting microalgae for biofuels production

    International Nuclear Information System (INIS)

    Highlights: • A low cost, high molecular weight cationic polymer was designed and developed for microalgal harvesting. • The polyamine polymer showed high flocculation efficiency for Scenedesmus sp. in comparison with chitosan and alum. • Such polymers could be preferred over other flocculants for microalgal harvesting for low value products such as biodiesel. • The polymer has not shown any deteriorating effect on lipid recovery and FAME profile of Scenedesmus sp. • Polyamine flocculant could be a cost effective option for harvesting of microalgal biomass for sustainable energy production. - Abstract: Research findings of the past few decades on the cultivation of microalgae for biodiesel production from laboratory to pilot scale microalgal cultivation have translated into empirical hope of developing an eco-friendly biofuel from algae. As far as economic sustainability is concerned, harvesting of microalgae is one of the most energy extensive processes and thus a major challenge, being faced by this industry. In our study, we designed and developed a quaternary ammonium salt based cationic polymer and evaluated its effectiveness for freshwater microalgae harvesting. An epichlorohydrin-n,n-diisopropylamine-dimethylamine polymer with high viscosity (1040 cps) was synthesized. The flocculation performance of this polyamine polymer was evaluated in terms of biomass recovery efficiency of microalgae (Scenedesmus sp.), its effect on lipid yield and composition. The results revealed that due to high molecular weight, the biomass recovery efficiency of the polymer was achieved >90% at a very small dose of 8 mg/L whereas similar biomass recovery efficiency of chitosan and alum were achieved at 80 and 250 mg/L respectively. The presence of functional quaternary amine and hydroxyl groups played an important role in electric charge neutralization of microalgal cells, hence the improved microalgal flocculation performance in comparison to the natural flocculants but

  12. Reduction of the THG emissions in agricultural productions for the generation of biofuels; Senkung der THG-Emissionen in landwirtschaftlichen Produktionsverfahren zur Erzeugung von Biokraftstoffen

    Energy Technology Data Exchange (ETDEWEB)

    Schiemenz, Katja; Gurgel, Andreas [Landesforschungsanstalt fuer Landwirtschaft und Fischerei Mecklenburg-Vorpommern, Guelzow-Pruezen (Germany). Inst. fuer Pflanzenproduktion und Betriebswirtschaft

    2013-10-01

    The Renewable Energy Directive (RED, 2009/128/EC) sets a binding goal of substituting at least 10% of fossil fuel consumption with renewable energy from 2020 onwards. Although biofuels of the second generation promise ecological and economic advantages, they are not yet available or (as with biomethane) available only to a very limited extent. It is therefore important to produce the currently available biofuels in a more environmentally friendly manner, particularly as biofuels must show a reduction in greenhouse gas (GHG) emissions relative to the fossil fuels they replace of 50% by 2017 and 60% by 2018 as per the German Biofuel Sustainability Ordinance. This concerns emissions from the whole biofuel production chain. In energy crop production the level of GHG emissions is particularly dependent on the amount of N fertilization and the intensity of soil tillage as well as indirectly on the amount of diesel consumption. A current LFA research project aims at the reduction of GHG field emissions in cultivation systems with energy crops (rape, ethanol wheat) for biofuel production. For this, the opportunities which arise from the use of crop rotation with multiple crop types appropriate for the location with the inclusion of N-fixing grain legumes and production technology should be grasped. (orig.)

  13. Production of Biofuels from Marine Macroalgae Melanothamnus afaqhusainii and Ulva fasciata

    International Nuclear Information System (INIS)

    This research article is based on the production of biofuels from the marine macro red alga Melanothamnus afaqhusainii and marine macro green alga Ulva fasciata from the Karachi coast. M. afaqhusainii was investigated for biodiesel as well as for bioethanol whereas U. fasciata was investigated only for bioethanol. Two types of biodiesels namely fatty acid methyl ester (FAME) and fatty acid ethyl ester (FAEE) were prepared by the transesterification of algal oil. The carbohydrates from both algae were extracted by distilled water and subjected to different biochemical tests. It was hydrolyzed by various methods including acidic and basic as well as by using ammonia, hydrogen peroxide and mixture of organic solvents in the presence of AlCl/sub 3/. The comparative study of these methods revealed their efficiency order as NaOH (10 percentage)/heating (method-2) > NaOH (10 percentage)/H/sub 2/O/sub 2/ in microwave oven (method-5) > ammonia (50 percentage)/reflux (method-6). After hydrolysis, the presence of carbohydrates in the hydrolyzed samples was investigated by different biochemical tests including Molisch Test, Iodine Test, Benedict Test, Tollen Test, Barfoed's Test, Fehling solution Test, Osazone Test, Seliwanoff's Test and Bial's Test. In addition, the presence of glucose was confirmed by glucometer and Combur Test. The detailed investigation of carbohydrates was carried out that resulted in the identification of reducing sugars, polysaccharides, disaccharides and monosaccharides including glucose. The hydrolyzed samples were fermented by Saccharomyces cerevisiae under ground and paratial anaerobic conditions. The bioethanol so formed by fermentation was directly converted to UV active derivatives namely ethyl benzoate and benzyl acetate. They were confirmed by TLC examination in comparison with standard ethyl benzoate and benzyl acetate. UV spectrophotometer was used for the determination of total sugar and bioethanol on the basis of absorbance

  14. Regional Environmental Impacts of Biofuel Feedstock Production--Scaling Biogeochemical Cycles in Space and Time

    Science.gov (United States)

    Vanloocke, A.; Bernacchi, C.

    2008-12-01

    Recently there has been increasing socio-economic and scientific interest in the use of alternative sources of energy to offset the negative effects of current fossil fuel dependence and consequent greenhouse gas emissions. Currently, one of the most popular alternatives is to use ethanol produced from domestically grown crops for use as fuel in the transportation sector. In 2007, over 7.5 billion gallons of ethanol were produced in the U.S. from corn, a traditional food crop. Recent research indicates that it may be logistically impractical, ecologically counterproductive (i.e. a net carbon source), and economically devastating to produce ethanol from crops previously grown to produce food. The EBI (Energy Biosciences Institute, at University of California Berkley and University of Illinois Urbana-Champaign) is now conducting research to assess the ability of traditional crops as well as dedicated biofuel feedstocks (e.g. Panicum virgatum (switchgrass), Miscanthus x Giganteus (Miscanthus), and Saccharum spp (sugar cane)) to provide a productive and sustainable alternative to fossil fuel. This is an important step to take before implementing the large-scale growth necessary to meet U.S. energy needs .A process-based terrestrial ecosystem model, Agro-IBIS (Agricultural Integrated Biosphere Simulator) was adapted to simulate the growth of Miscanthus. The model was calibrated using data collected from sites at the University of Illinois south farms. Simulations indicated significant implications on the regional carbon and water budgets. Next this locally validated method will be extrapolated to simulate the regional scale growth of Miscanthus in the Midwestern U.S. and sugarcane in Brazil and a similar analysis will be conducted for switchgrass. The results should provide insight on optimal land-use decisions and legislation that regard meeting energy demands and mitigating climate change in the near future.

  15. NREL's Cyanobacteria Engineering Shortens Biofuel Production Process, Captures CO2

    Energy Technology Data Exchange (ETDEWEB)

    2015-09-01

    This highlight describes NREL's work to systematically analyze the flow of energy in a photosynthetic microbe and show how the organism adjusts its metabolism to meet the increased energy demand for making ethylene. This work successfully demonstrates that the organism could cooperate by stimulating photosynthesis. The results encourage further genetic engineering for the conversion of CO2 to biofuels and chemicals. This highlight is being developed for the September 2015 Alliance S&T Board meeting. biofuels and chemicals. This highlight is being developed for the September 2015 Alliance S&T Board meeting.

  16. Conditions for resource-efficient production of biofuels for transport in Sweden

    OpenAIRE

    Ersson, Carolina

    2014-01-01

    Transportation has continued to increase worldwide and fossil-fuel dependency is strong which leads to a number of problems, e.g. increased emissions of green-house gases (GHG) and risks related to energy security. Biofuels have until now been one of the few renewable alternatives which have been able to replace fossil fuels on a large scale. The biofuel share in relation to the total use of fuel in the transportation sector is still small, but in many places in the world political targets ar...

  17. Solid biofuels production from agricultural residues and processing by-products by means of torrefaction treatment: the case of sunflower chain

    OpenAIRE

    Daniele Duca; Giovanni Riva; Ester Foppa Pedretti; Giuseppe Toscano; Chiara Mengarelli; Giorgio Rossini

    2014-01-01

    The high heterogeneity of some residual biomasses makes rather difficult their energy use. Their standardisation is going to be a key aspect to get good quality biofuels from those residues. Torrefaction is an interesting process to improve the physical and chemical properties of lignocellulosic biomasses and to achieve standardisation. In the present study torrefaction has been employed on residues and by-products deriving from sunflower production chain, in particular sunflower stalks, husk...

  18. N2O release from agro-biofuel production negates global warming reduction by replacing fossil fuels

    Science.gov (United States)

    Crutzen, P. J.; Mosier, A. R.; Smith, K. A.; Winiwarter, W.

    2008-01-01

    The relationship, on a global basis, between the amount of N fixed by chemical, biological or atmospheric processes entering the terrestrial biosphere, and the total emission of nitrous oxide (N2O), has been re-examined, using known global atmospheric removal rates and concentration growth of N2O as a proxy for overall emissions. For both the pre-industrial period and in recent times, after taking into account the large-scale changes in synthetic N fertiliser production, we find an overall conversion factor of 3-5% from newly fixed N to N2O-N. We assume the same factor to be valid for biofuel production systems. It is covered only in part by the default conversion factor for "direct" emissions from agricultural crop lands (1%) estimated by IPCC (2006), and the default factors for the "indirect" emissions (following volatilization/deposition and leaching/runoff of N: 0.35-0.45%) cited therein. However, as we show in the paper, when additional emissions included in the IPCC methodology, e.g. those from livestock production, are included, the total may not be inconsistent with that given by our "top-down" method. When the extra N2O emission from biofuel production is calculated in "CO2-equivalent" global warming terms, and compared with the quasi-cooling effect of "saving" emissions of fossil fuel derived CO2, the outcome is that the production of commonly used biofuels, such as biodiesel from rapeseed and bioethanol from corn (maize), depending on N fertilizer uptake efficiency by the plants, can contribute as much or more to global warming by N2O emissions than cooling by fossil fuel savings. Crops with less N demand, such as grasses and woody coppice species, have more favourable climate impacts. This analysis only considers the conversion of biomass to biofuel. It does not take into account the use of fossil fuel on the farms and for fertilizer and pesticide production, but it also neglects the production of useful co-products. Both factors partially compensate

  19. N2O release from agro-biofuel production negates global warming reduction by replacing fossil fuels

    Directory of Open Access Journals (Sweden)

    A. R. Mosier

    2008-01-01

    Full Text Available The relationship, on a global basis, between the amount of N fixed by chemical, biological or atmospheric processes entering the terrestrial biosphere, and the total emission of nitrous oxide (N2O, has been re-examined, using known global atmospheric removal rates and concentration growth of N2O as a proxy for overall emissions. For both the pre-industrial period and in recent times, after taking into account the large-scale changes in synthetic N fertiliser production, we find an overall conversion factor of 3–5% from newly fixed N to N2O-N. We assume the same factor to be valid for biofuel production systems. It is covered only in part by the default conversion factor for "direct" emissions from agricultural crop lands (1% estimated by IPCC (2006, and the default factors for the "indirect" emissions (following volatilization/deposition and leaching/runoff of N: 0.35–0.45% cited therein. However, as we show in the paper, when additional emissions included in the IPCC methodology, e.g. those from livestock production, are included, the total may not be inconsistent with that given by our "top-down" method. When the extra N2O emission from biofuel production is calculated in "CO2-equivalent" global warming terms, and compared with the quasi-cooling effect of "saving" emissions of fossil fuel derived CO2, the outcome is that the production of commonly used biofuels, such as biodiesel from rapeseed and bioethanol from corn (maize, depending on N fertilizer uptake efficiency by the plants, can contribute as much or more to global warming by N2O emissions than cooling by fossil fuel savings. Crops with less N demand, such as grasses and woody coppice species, have more favourable climate impacts. This analysis only considers the conversion of biomass to biofuel. It does not take into account the use of fossil fuel on the farms and for fertilizer and pesticide production, but it also neglects the production of useful co-products. Both factors

  20. Competition between biofuels. Modeling technological learning and cost reductions over time

    International Nuclear Information System (INIS)

    A key aspect in modeling the (future) competition between biofuels is the way in which production cost developments are computed. The objective of this study was threefold: (1) to construct a (endogenous) relation between cost development and cumulative production (2) to implement technological learning based on both engineering study insights and an experience curve approach, and (3) to investigate the impact of different technological learning assumptions on the market diffusion patterns of different biofuels. The analysis was executed with the European biofuel model BioTrans, which computes the least cost biofuel route. The model meets an increasing demand, reaching a 25% share of biofuels of the overall European transport fuel demand by 2030. Results show that 1st generation biodiesel is the most cost competitive fuel, dominating the early market. With increasing demand, modestly productive oilseed crops become more expensive rapidly, providing opportunities for advanced biofuels to enter the market. While biodiesel supply typically remains steady until 2030, almost all additional yearly demands are delivered by advanced biofuels, supplying up to 60% of the market by 2030. Sensitivity analysis shows that (a) overall increasing investment costs favour biodiesel production, (b) separate gasoline and diesel subtargets may diversify feedstock production and technology implementation, thus limiting the risk of failure and preventing lock-in and (c) the moment of an advanced technology's commercial market introduction determines, to a large degree, its future chances for increasing market share.

  1. Competition between biofuels: Modeling technological learning and cost reductions over time

    International Nuclear Information System (INIS)

    A key aspect in modeling the (future) competition between biofuels is the way in which production cost developments are computed. The objective of this study was threefold: (i) to construct a (endogenous) relation between cost development and cumulative production (ii) to implement technological learning based on both engineering study insights and an experience curve approach, and (iii) to investigate the impact of different technological learning assumptions on the market diffusion patterns of different biofuels. The analysis was executed with the European biofuel model BioTrans, which computes the least cost biofuel route. The model meets an increasing demand, reaching a 25% share of biofuels of the overall European transport fuel demand by 2030. Results show that 1st generation biodiesel is the most cost competitive fuel, dominating the early market. With increasing demand, modestly productive oilseed crops become more expensive rapidly, providing opportunities for advanced biofuels to enter the market. While biodiesel supply typically remains steady until 2030, almost all additional yearly demands are delivered by advanced biofuels, supplying up to 60% of the market by 2030. Sensitivity analysis shows that (i) overall increasing investment costs favour biodiesel production, (ii) separate gasoline and diesel subtargets may diversify feedstock production and technology implementation, thus limiting the risk of failure and preventing lock-in and (iii) the moment of an advanced technology's commercial market introduction determines, to a large degree, its future chances for increasing market share. (author)

  2. Assessment of a Novel Algal Strain Chlamydomonas debaryana NIREMACC03 for Mass Cultivation, Biofuels Production and Kinetic Studies.

    Science.gov (United States)

    Mishra, Sanjeev; Singh, Neetu; Sarma, Anil Kumar

    2015-08-01

    A novel microalgae strain Chlamydomonas debaryana (KJ210856) was isolated from a freshwater lake of Punjab, India, and cultivated considering climatic sustainability and inherent adaptability concern. C. debaryana was grown in a 30-L indoor photobioreactor to study the mass cultivation prospect and biofuel potential. Physicochemical characterization of biomass and the lipid was performed with effect to nitrogen stress. It showed a higher biomass yield (1.58 ± 0.02 g L(-1), dry weight) and twofold increase in lipid yield (552.78 ± 9 mg L(-1)) with 34.2 ± 0.19 % lipid content under nitrogen deficient condition. Strikingly, increase in triglycerides achieved with nitrogen depletion containing over 96 % of total fatty acids (C 14, C 16, and C 18). Proximate and ultimate analysis suggested the presence of relatively higher volatile matter and carbon-hydrogen ratio. Furthermore, lower moisture and ash content signified C. debaryana biomass has promising features towards biofuel applications. The pyrolytic behavior of the whole biomass was also studied using thermogravimetric analyzer (TGA) and kinetic parameters were estimated using different methods. Promising growth rate and lipid yield leading to feasible biofuel feed stock production in indoor photobioreactor along with autosediment potential of cells validates C. debaryana NIREMACC03, a potential strain for mass cultivation. PMID:26093613

  3. Biofuel and other biomass based products from contaminated sites - Potentials and barriers from Swedish perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Andersson-Skoeld, Yvonne; Enell, Anja; Rihm, Thomas; Haglund, Kristina; Wik, Ola (Swedish Geotechnical Institute, Linkoeping (Sweden)); Blom, Sonja; Angelbratt, Alexandra (FB Engineering AB, Goeteborg (Sweden)); Bardos, Paul (r3 Environmental Technology Ltd, Reading (United Kingdom)); Track, Thomas (DECHEMA e. V., Frankfurt am Main (Germany)); Keuning, Sytze (Bioclear b.v., Groningen (Netherlands))

    2009-07-01

    In this report, results are presented based on interviews and literature surveys on the triggers and stoppers for non food crop on contaminated land in Sweden. The report also includes a first estimate of potential marginal land for biofuel production in Sweden. The report is a first step to explore the feasibility of a range of possible approaches to combine risk based land management (RBLM) with non-food crop land-uses and organic matter re-use as appropriate in a Swedish context. The focus of the report is on the treatment of contaminated land by phyto-remediation and on biofuel cultivation. In Sweden, like all other countries in Europe, areas of land have been degraded by past use. Such previously developed land includes areas affected by mining, fallout from industrial processes such as smelting, areas elevated with contaminated dredged sediments, former landfill sites and many other areas where the decline of industrial activity has left a legacy of degraded land and communities. The extent of contamination may not be sufficient to trigger remediation under current regulatory conditions, and there may be little economic incentive to regenerate the affected areas. An ideal solution would be a land management approach that is able to pay for itself. Biomass from coppice or other plantations has long been seen as a possible means of achieving this goal. Phyto remediation offers a low cost method for remediation of areas that are not candidates for conventional regeneration. The optimal conditions for phyto remediation are large land areas of low or mediate contamination. Phyto remediation is also suitable to prevent spreading of contaminants, for example in green areas such as in cities, as waste water buffer and small size remediation areas with diffuse spreading. Phyto remediation implies that plants, fungi or algae are used to remediate, control or increase the natural attenuation of contaminants. Depending on the contaminating species and the site conditions

  4. 75 FR 44015 - Certain Semiconductor Products Made by Advanced Lithography Techniques and Products Containing...

    Science.gov (United States)

    2010-07-27

    ... COMMISSION Certain Semiconductor Products Made by Advanced Lithography Techniques and Products Containing... importation of certain semiconductor products made by advanced lithography techniques and products containing... certain semiconductor products made by advanced lithography techniques or products containing same...

  5. Weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'Biofuel'.

    Science.gov (United States)

    Chandel, Anuj K; Singh, Om V

    2011-03-01

    Lignocellulosic materials are the most abundant renewable organic resources (~200 billion tons annually) on earth that are readily available for conversion to ethanol and other value-added products, but they have not yet been tapped for the commercial production of fuel ethanol. The lignocellulosic substrates include woody substrates such as hardwood (birch and aspen, etc.) and softwood (spruce and pine, etc.), agro residues (wheat straw, sugarcane bagasse, corn stover, etc.), dedicated energy crops (switch grass, and Miscanthus etc.), weedy materials (Eicchornia crassipes, Lantana camara etc.), and municipal solid waste (food and kitchen waste, etc.). Despite the success achieved in the laboratory, there are limitations to success with lignocellulosic substrates on a commercial scale. The future of lignocellulosics is expected to lie in improvements of plant biomass, metabolic engineering of ethanol, and cellulolytic enzyme-producing microorganisms, fullest exploitation of weed materials, and process integration of the individual steps involved in bioethanol production. Issues related to the chemical composition of various weedy raw substrates for bioethanol formation, including chemical composition-based structural hydrolysis of the substrate, need special attention. This area could be opened up further by exploring genetically modified metabolic engineering routes in weedy materials and in biocatalysts that would make the production of bioethanol more efficient. PMID:21181146

  6. Stakeholder perceptions of biofuels from microalgae

    International Nuclear Information System (INIS)

    In this paper we focus on stakeholder views around the development of advanced biofuels from microalgae. Research for the development of microalgal-derived biofuels was initiated by the US Department of Energy (DOE) more than 30 years ago. However, interest in this eco-innovation has been growing significantly over the last five years in various countries. The high productivity of algae indicates that algal biofuels could contribute to reduce our dependence on fossil fuels while avoiding the undesired impacts of first generation biofuels. There are still numerous challenges that need nevertheless to be addressed. The aim of this paper is to explore stakeholder perceptions of the current barriers and opportunities associated with this promising emergent technology. - Research highlights: → There are still many questions about how microalgae biofuels will reduce our demand of fossil fuels. → Many of the obstacles discussed by participants are related to the technological and economic barriers. → There are still competing visions on the aspects of the technology where efforts have to be applied. → Years of intensive, continuous and large-scale research and a favorable environment are needed. → The careful protection of the innovation may avoid failure experiences.

  7. Vermont Biofuels Initiative: Local Production for Local Use to Supply a Portion of Vermont's Energy Needs

    Energy Technology Data Exchange (ETDEWEB)

    Sawyer, Scott; Kahler, Ellen

    2009-05-31

    The Vermont Biofuels initiative (VBI) is the Vermont Sustainable Jobs Fund's (VSJF) biomass-to-biofuels market development program. Vermont is a small state with a large petroleum dependency for transportation (18th in per capita petroleum consumption) and home heating (55% of all households use petroleum for heating). The VBI marks the first strategic effort to reduce Vermont's dependency on petroleum through the development of homegrown alternatives. As such, it supports the four key priorities of the U.S. Department of Energy's Multi-year Biomass Plan: 1.) Dramatically reduce dependence on foreign oil; 2.) Promote the use of diverse, domestic and sustainable energy resources; 3.) Reduce carbon emissions from energy production and consumption; 4.) Establish a domestic bioindustry. In 2005 VSJF was awarded with a $496,000 Congressionally directed award from U.S. Senator Patrick Leahy. This award was administered through the U.S. Department of Energy (DE-FG36- 05GO85017, hereafter referred to as DOE FY05) with $396,000 to be used by VSJF for biodiesel development and $100,000 to be used by the Vermont Department of Public Service for methane biodigester projects. The intent and strategic focus of the VBI is similar to another DOE funded organization-the Biofuels Center of North Carolina-in that it is a nonprofit driven, statewide biofuels market development effort. DOE FY05 funds were expensed from 2006 through 2008 for seven projects: 1) a feedstock production, logistics, and biomass conversion research project conducted by the University of Vermont Extension; 2) technical assistance in the form of a safety review and engineering study of State Line Biofuels existing biodiesel production facility; 3) technical assistance in the form of a safety review and engineering study of Borderview Farm's proposed biodiesel production facility; 4) technology and infrastructure purchases for capacity expansion at Green Technologies, LLC, a waste vegetable

  8. The biofuels, situation, perspectives

    International Nuclear Information System (INIS)

    The climatic change with the fight against the greenhouse effect gases, sees the development of ''clean'' energy sources. Meanwhile the biofuels remain penalized by their high production cost, the interest is increasing. Facing their development ecologists highlight the environmental and social negative impacts of the development of the biofuels. The author aims to take stock on the techniques and the utilizations. (A.L.B.)

  9. Small-scale production of straight vegetable oil from rapeseed and its use as biofuel in the Spanish territory

    International Nuclear Information System (INIS)

    Biofuels nowadays are an important topic of study. The most significant point is the availability of bioethanol or biodiesel and their production from different raw materials. It is already known that large-scale production of first-generation biodiesel cannot be seen as an alternative to fossil fuels due to land requirements, competition with food, increase in fertilizer requirements and pressure on tropical forests among others. This fact does not necessarily apply to second-generation biofuels or small-scale niche productions. Straight vegetable oil (SVO) can be used directly in diesel engines with minor modifications. Our proposal is a small-scale SVO production system for self-supply in agricultural machinery. In this paper a model to provide SVO to local farmers in a specific area in Catalonia (Spain) is presented. We also present a discussion about the regulations to be changed in order to make possible the incorporation of SVO as engine fuel in diesel vehicles and a comparative analysis between the emissions of tractors fed with SVO and petrodiesel. Moreover, a quantitative economic analysis of modifying diesel engines and long-term operability costs are shown and a first-run economic analysis comparing the actual crop rotation with the proposed one and some alternatives is studied.

  10. The role of CAFE standards and alternative-fuel vehicle production credits in U.S. biofuels markets

    International Nuclear Information System (INIS)

    This paper investigates the impact of CAFE standards and alternative-fuel vehicle production incentives on the biofuel market and mandate, in particular. The study develops a structural model of the domestic light-duty vehicle sector, as well as reduced-form versions of domestic gasoline, diesel, and biofuel markets. The results suggest that holding CAFE standards at the 2010 level could facilitate U.S. ethanol market expansion, making it easier to meet the mandate. Alternative-fuel vehicle production incentives appear to have small effects. However, there is uncertainty about the level of automaker response to those incentives, and analysis indicates the model is fairly sensitive to the assumed level of response. - Highlights: • CAFE standards are estimated to have a moderate impact on RFS compliance costs. • Flex-fuel vehicle production incentives are estimated to have much less impact. • Level of auto manufacturer response to production incentives is uncertain. • Analysis suggests results are fairly sensitive to level of manufacturer response

  11. Engineering metabolic systems for production of advanced fuels

    OpenAIRE

    Yan, Yajun; Liao, James C.

    2009-01-01

    The depleting petroleum storage and increasing environmental deterioration are threatening the sustainable development of human societies. As such, biofuels and chemical feedstocks generated from renewable sources are becoming increasingly important. Although previous efforts led to great success in bio-ethanol production, higher alcohols, fatty acid derivatives including biodiesels, alkanes, and alkenes offer additional advantages because of their compatibility with existing infrastructure. ...

  12. Advances in solar silicon production

    International Nuclear Information System (INIS)

    The results of carbothermic reduction experiments carried out at a preindustrial scale with a 240 KVA submerged are furnace fed with pellets of carbon black and silica sand are reported and discussed. It was demonstrated that all powder agglomeration techniques used to fabricate the pellets (pellettizing, briquetting, extrusion) give rise to small or negligible contamination of the charge. The results of experiments support the view that furnace handling is a powerful source of impurity contamination. Contamination from furnace lining could be however controlled and rendered negligible by an improved furnace design and proper choice of refractory materials. Graphite or graphite coated tools could eventually improve also the furnace tapping and handling operations. The solution of these problems, which is in substantial progress in this case, presents however additional problems in the case of larger size furnace and presumibly will limit the size of furnaces for PMS production to 500-1000 KVA. As the authors demonstrated that the boron and phosphorous is not influenced by furnace operation, one can conclude that the use of the granulated lump quartz of higher purity quartz sands could reconduct our PMS within the specifications of a solar grade material

  13. Biofuel alternatives to ethanol: pumping the microbial well

    Energy Technology Data Exchange (ETDEWEB)

    Fortman, J.L.; Chhabra, Swapnil; Mukhopadhyay, Aindrila; Chou, Howard; Lee, Taek Soon; Steen, Eric; Keasling, Jay D.

    2009-08-19

    Engineered microorganisms are currently used for the production of food products, pharmaceuticals, ethanol fuel and more. Even so, the enormous potential of this technology has yet to be fully exploited. The need for sustainable sources of transportation fuels has generated a tremendous interest in technologies that enable biofuel production. Decades of work have produced a considerable knowledge-base for the physiology and pathway engineering of microbes, making microbial engineering an ideal strategy for producing biofuel. Although ethanol currently dominates the biofuel market, some of its inherent physical properties make it a less than ideal product. To highlight additional options, we review advances in microbial engineering for the production of other potential fuel molecules, using a variety of biosynthetic pathways.

  14. Biofuel alternatives to ethanol: pumping the microbial well

    Energy Technology Data Exchange (ETDEWEB)

    Fortman, J. L.; Chhabra, Swapnil; Mukhopadhyay, Aindrila; Chou, Howard; Lee, Taek Soon; Steen, Eric; Keasling, Jay D.

    2009-12-02

    Engineered microorganisms are currently used for the production of food products, pharmaceuticals, ethanol fuel and more. Even so, the enormous potential of this technology has yet to be fully exploited. The need for sustainable sources of transportation fuels has gener-ated a tremendous interest in technologies that enable biofuel production. Decades of work have produced a considerable knowledge-base for the physiology and pathway engineering of microbes, making microbial engineering an ideal strategy for producing biofuel. Although ethanol currently dominates the biofuel mar-ket, some of its inherent physical properties make it a less than ideal product. To highlight additional options, we review advances in microbial engineering for the production of other potential fuel molecules, using a variety of biosynthetic pathways.

  15. Biofuels and biodiversity.

    Science.gov (United States)

    Wiens, John; Fargione, Joseph; Hill, Jason

    2011-06-01

    The recent increase in liquid biofuel production has stemmed from a desire to reduce dependence on foreign oil, mitigate rising energy prices, promote rural economic development, and reduce greenhouse gas emissions. The growth of this industry has important implications for biodiversity, the effects of which depend largely on which biofuel feedstocks are being grown and the spatial extent and landscape pattern of land requirements for growing these feedstocks. Current biofuel production occurs largely on croplands that have long been in agricultural production. The additional land area required for future biofuels production can be met in part by reclaiming reserve or abandoned croplands and by extending cropping into lands formerly deemed marginal for agriculture. In the United States, many such marginal lands have been enrolled in the Conservation Reserve Program (CRP), providing important habitat for grassland species. The demand for corn ethanOl has changed agricultural commodity economics dramatically, already contributing to loss of CRP lands as contracts expire and lands are returned to agricultural production. Nevertheless, there are ways in which biofuels can be developed to enhance their coexistence with biodiversity. Landscape heterogeneity can be improved by interspersion of land uses, which is easier around facilities with smaller or more varied feedstock demands. The development of biofuel feedstocks that yield high net energy returns with minimal carbon debts or that do not require additional land for production, such as residues and wastes, should be encouraged. Competing land uses, including both biofuel production and biodiversity protection, should be subjected to comprehensive cost-benefit analysis, so that incentives can be directed where they will do the most good.

  16. Biofuel market and carbon modeling to evaluate French biofuel policy

    International Nuclear Information System (INIS)

    In order to comply with European objectives, France has set up an ambitious biofuel plan. This plan is evaluated considering two criteria: tax exemption need and GHG emission savings. An economic marginal analysis and a life cycle assessment (LCA) are provided using a coupling procedure between a partial agro-industrial equilibrium model and a refining optimization model. Thus, we are able to determine the minimum tax exemption needed to place on the market a targeted quantity of biofuel by deducing the agro-industrial marginal cost of biofuel production to the biofuel refining long-run marginal revenue. In parallel, a biofuels LCA is carried out using model outputs. Such a method avoid common allocation problems between joint products. The French biofuel plan is evaluated for 2008, 2010 and 2012 using prospective scenarios. Results suggest that biofuel competitiveness depends on crude oil prices and petroleum products demands. Consequently, biofuel tax exemption does not always appear to be necessary. LCA results show that biofuels production and use, from 'seed to wheel', would facilitate the French Government's to compliance with its 'Plan Climat' objectives by reducing up to 5% GHG emissions in the French road transport sector by 2010. (authors)

  17. Making biofuels sustainable

    International Nuclear Information System (INIS)

    Full text: As the twentieth century drew to a close, there was considerable support for the use of biofuels as a source of renewable energy. To many people, they offered significant savings in greenhouse gas emissions compared to fossil fuels, an opportunity for reduced dependency on oil for transport, and potential as a counter weight to increasing oil prices. They also promised an opportunity for rural economies to benefit from a new market for their products and a chance of narrowing the gap between rich and poor nations. Biofuel development was encouraged by government subsidies, and rapid growth occurred in many parts of the world. Forty per cent of Brazilian sugar cane is used for biofuel production, for example, as is almost a quarter of maize grown in the United States. Although only around 1 per cent of arable land is cultivated to grow feedstock for biofuels, there has been increasing concern over the way a largely unchecked market has developed, and about its social and environmental consequences. Recent research has confirmed that food prices have been driven significantly higher by competition for prime agricultural land and that savings in greenhouse gas emissions are much smaller - and in some cases entirely eliminated - when environmentally important land, such as rainforest, is destroyed to grow biofuels. As a result, many now believe that the economic benefits of biofuels have been obtained at too high a social and environmental price, and they question whether they can be a truly sustainable source of energy. The United Kingdom has always had sustainability at the heart of its biofuel policies and set up the Renewable Fuels Agency to ensure that this goal was met. The direct effects of biofuel production are already being assessed through five measures of environmental performance and two measures of social performance, as well as measures of the energy efficiency of the production processes used and of the greenhouse gas savings achieved

  18. Turning Bacteria into Fuel: Cyanobacteria Designed for Solar-Powered Highly Efficient Production of Biofuels

    Energy Technology Data Exchange (ETDEWEB)

    None

    2010-01-01

    Broad Funding Opportunity Announcement Project: ASU is engineering a type of photosynthetic bacteria that efficiently produce fatty acids—a fuel precursor for biofuels. This type of bacteria, called Synechocystis, is already good at converting solar energy and carbon dioxide (CO2) into a type of fatty acid called lauric acid. ASU has modified the organism so it continuously converts sunlight and CO2 into fatty acids—overriding its natural tendency to use solar energy solely for cell growth and maximizing the solar-to-fuel conversion process. ASU’s approach is different because most biofuels research focuses on increasing cellular biomass and not on excreting fatty acids. The project has also identified a unique way to convert the harvested lauric acid into a fuel that can be easily blended with existing transportation fuels.

  19. Isolation, screening and characterization of microorganisms with potential for biofuels production

    OpenAIRE

    Teixeira, Pedro Jorge Dias, 1989-

    2012-01-01

    Tese de mestrado. Biologia (Microbiologia Aplicada). Universidade de Lisboa, Faculdade de Ciências, 2012 Rapid global population growth has increased the demand for food and energy supply. The limited oil reserves, pollution concerns, global warming and political instability and disagreements, lead to an increased financial support for sustainable and environmental sources of energy, biofuels. In the last decades there is an increasing interest in the development of the bioethanol producti...

  20. The effect of seasonal variation on biomethane production from seaweed and on application as a gaseous transport biofuel.

    Science.gov (United States)

    Tabassum, Muhammad Rizwan; Xia, Ao; Murphy, Jerry D

    2016-06-01

    Biomethane produced from seaweed may be used as a transport biofuel. Seasonal variation will have an effect on this industry. Laminaria digitata, a typical Irish brown seaweed species, shows significant seasonal variation both in proximate, ultimate and biochemical composition. The characteristics in August were optimal with the lowest level of ash (20% of volatile solids), a C:N ratio of 32 and the highest specific methane yield measured at 327LCH4kgVS(-1), which was 72% of theoretical yield. The highest yield per mass collected of 53m(3)CH4t(-1) was achieved in August, which is 4.5 times higher than the lowest value, obtained in December. A seaweed cultivation area of 11,800ha would be required to satisfy the 2020 target for advanced biofuels in Ireland, of 1.25% renewable energy supply in transport (RES-T) based on the optimal gross energy yield obtained in August (200GJha(-1)yr(-1)). PMID:26970924

  1. The production of biofuels in Germany. State of the art and optimization approaches; Die Biokraftstoffproduktion in Deutschland. Stand der Technik und Optimierungsansaetze

    Energy Technology Data Exchange (ETDEWEB)

    Braune, Maria; Groengroeft, Arne [DBFZ Deutsches Biomasseforschungszentrum gemeinnuetzige GmbH, Leipzig (Germany); Oehmichen, Katja; Zech, Konstantin

    2015-07-01

    Determining the status quo of existing biofuel plants in Germany, an extensive database of process configurations was received. This information was used to develop simulation models of virtual biofuel plants, which are now available for further investigations. The GHG emissions of biofuels can partly be improved by the studied optimization approaches. The studied optimization approaches can hence contribute to the increasing requirements for GHG reductions of biofuels. The integration of multiple optimization approaches in one concept would improve its GHG balance further. In addition to the investigated technical measures, emissions could also be reduced by adapting the energy supply. The use of biofuels for heat generation, for example, could have an additional reducing effect on total GHG emissions, because a fossil fuel-based heat and electricity supply is the main driver of the GHG balance. From the communication with contacts in the industry it can be said that the German biofuel industry operates production plants that have already reached a high level of efficiency. At many locations, additional measures and modifications are planned, not only driven by economic reasons but also to further improve the GHG balance. The overall results of the project will be published as DBFZ Report No. 22 ''Die Biokraftstoffproduktion in Deutschland - Stand der Technik und Optimierungsansaetze'' in 2015.

  2. Biofuels in Oregon and Washington: A Business Case Analysis of Opportunities and Challenges

    Energy Technology Data Exchange (ETDEWEB)

    Stiles, Dennis L.; Jones, Susan A.; Orth, Rick J.; Saffell, Bernard F.; Zhu, Yunhua

    2008-02-28

    The purpose of this report is to assemble the information needed to estimate the significance of the opportunity for producing biofuels in the region as well as the associated challenges. The report reviews the current state of the industry, the biomass resources that are available within current production practices, and the biofuels production technology that is available within the marketplace. The report also identifys the areas in which alternative approaches or strategies, or technologoical advances, might offer an opportunity to expand the Nortwest biofuels industry beyond its current state.

  3. Bio-fuels

    International Nuclear Information System (INIS)

    This report presents an overview of the technologies which are currently used or presently developed for the production of bio-fuels in Europe and more particularly in France. After a brief history of this production since the beginning of the 20. century, the authors describe the support to agriculture and the influence of the Common Agricultural Policy, outline the influence of the present context of struggle against the greenhouse effect, and present the European legislative context. Data on the bio-fuels consumption in the European Union in 2006 are discussed. An overview of the evolution of the activity related to bio-fuels in France, indicating the locations of ethanol and bio-diesel production facilities, and the evolution of bio-fuel consumption, is given. The German situation is briefly presented. Production of ethanol by fermentation, the manufacturing of ETBE, the bio-diesel production from vegetable oils are discussed. Second generation bio-fuels are then presented (cellulose enzymatic processing), together with studies on thermochemical processes and available biomass resources

  4. Application of Ionic Liquid [DMIM]DMP Pretreatment in the Hydrolysis of Sugarcane Bagasse for Biofuel Production

    Directory of Open Access Journals (Sweden)

    Arief Widjaja

    2015-03-01

    Full Text Available Sugarcane bagasse is one of lignocellulose materials that can be converted to biofuel. This work was aimed to develop new pretreatment combination methods to process sugarcane bagasse lignocellulose into biofuel (bio-hydrogen. Pretreatment of sugarcane bagasse using NaOH solution in combination with ionic liquid [DMIM]DMP enhanced the enzymatic hydrolysis significantly. After the pretreat-ment, the content of cellulose and hemicellulose increased by 29.31% compared to the untreated one. Cellulose and hemicelluloses were used as raw materials to produce reducing sugars, that can be con-verted to bio-hydrogen via fermentation. After being subjected to combined pretreatment processes, the crystalline index of sugarcane bagasse decreased significantly compared to solely NaOH pretratment. This indicates a more amorphous structure of the sugarcane bagasse, which makes it is easier to be hy-drolyzed into reducing sugars. The recovery of cellulose + hemicellulose after pretreatment for 20 min and 120 °C was 92%, and the yield obtained was 0.556 g sugars/g (cellulose + hemicellulose after 12 h and the bio-hydrogen yield was 0.46 mol H2/mol sugars consumed after 48 h fermentation. The use of recycled of ionic liquid showed similar performance compared to the use of fresh ionic liquid. © 2015 BCREC UNDIP. All rights reservedReceived: 21st July 2014; Revised: 15th September 2014; Accepted: 12nd December 2014How to Cite: Widjaja, A., Agnesty, S.Y., Sangian, H.F., Gunawan, S. (2014. Application of Ionic Liquid [DMIM]DMP Pretreatment in the Hydrolysis of Sugarcane Bagasse for Biofuel Production. Bulletin of Chemical Reaction Engineering & Catalysis, 10 (1: 70-77. (doi:10.9767/bcrec.10.1.7143.70-77Permalink/DOI: http://dx.doi.org/10.9767/bcrec.10.1.7143.70-77 

  5. Biofuels and Biotechnology

    Energy Technology Data Exchange (ETDEWEB)

    Mielenz, Jonathan R [ORNL

    2009-01-01

    The world obtains 86% of its energy from fossil fuels, 40% from petroleum, a majority of which goes to the transportation sector (www.IEA.gov). Well-recognized alternatives are fuels derived from renewable sources known as biofuels. There are a number of biofuels useful for transportation fuels, which include ethanol, biobutanol, mixed alcohols, biodiesel, and hydrogen. These biofuels are produced from biologically derived feedstock, almost exclusively being plant materials, either food or feed sources or inedible plant material called biomass. This chapter will discuss technologies for production of liquid transportation biofuels from renewable feedstocks, but hydrogen will not be included, as the production technology and infrastructure are not near term. In addition, a specific emphasis will be placed upon the research opportunities and potential for application of system biology tools to dissect and understand the biological processes central to production of these biofuels from biomass and biological materials. There are a number of technologies for production of each of these biofuels that range from fully mature processes such as grain-derived ethanol, emerging technology of ethanol form cellulose derived ethanol and immature processes such thermochemical conversion technologies and production of hydrogen all produced from renewable biological feedstocks. Conversion of biomass by various thermochemical and combustion technologies to produce thermochemical biodiesel or steam and electricity provide growing sources of bioenergy. However, these technologies are outside of the scope of this chapter, as is the use of biological processing for upgrading and conversion of fossil fuels. Therefore, this chapter will focus on the current status of production of biofuels produced from biological-derived feedstocks using biological processes. Regardless of the status of development of the biological process for production of the biofuels, each process can benefit from

  6. Increase of Biofuel Crop Production in Romania over the Last Decades - Possible Impacts on Environment, Greenhouse Gas Emissions and Land Use

    Directory of Open Access Journals (Sweden)

    Vasilica STAN

    2014-12-01

    Full Text Available The interest in biofuels is growing globally and the demand for bioenergy throughout the European Union will expand considerably in the following years. Numerous studies have shown that energy resulted from green plants has much to offer, as it is renewable and largely carbon-neutral compared with fossil fuel combustion. However, this is an increasingly controversial issue due to its strong impact on economy, environment and land use. Romania is one of the European Union Member States with a large biofuel production resources, owing to its high land agricultural area and good conditions for cereal and oilseed growth. There has been an increasing tendency to grow biofuels in the Romanian cultivated areas over the last decades; however, it is difficult to know if biofuel production is sustainable in this part of Europe and what impact may have unsustainable production since there is no or scientific research on this topic. The aim of this paper is to present the dynamics of biofuel crops (biodiesel areas in Romania over the last decade, and to introduce a short review on the possible impacts on the environment, greenhouse gas emissions (GES and land use in order to defend the need of information for our particular situation.

  7. Lignocellulosic residues for production of electricity, biogas or second generation biofuel: A case study of technical and sustainable potential of rice straw in Mali

    DEFF Research Database (Denmark)

    Nygaard, Ivan; Dembelé, Filifing; Daou, Ibrahima;

    2016-01-01

    Biomass from agricultural residues, especially lignocellulosic biomass, is not only seen as a sustainable biomass source for the production of electricity, but increasingly as a resource for the production of biogas and second generation biofuel in developing countries. Based on empirical researc...

  8. Microalgae biofuel potentials (review).

    Science.gov (United States)

    Ghasemi, Y; Rasoul-Amini, S; Naseri, A T; Montazeri-Najafabady, N; Mobasher, M A; Dabbagh, F

    2012-01-01

    With the decrease of fossil based fuels and the environmental impact of them over the planet, it seems necessary to seek the sustainable sources of clean energy. Biofuels, is becoming a worldwide leader in the development of renewable energy resources. It is worthwhile to say that algal biofuel production is thought to help stabilize the concentration of carbon dioxide in the atmosphere and decrease global warming impacts. Also, among algal fuels' attractive characteristics, algal biodiesel is non toxic, with no sulfur, highly biodegradable and relatively harmless to the environment if spilled. Algae are capable of producing in excess of 30 times more oil per acre than corn and soybean crops. Currently, algal biofuel production has not been commercialized due to high costs associated with production, harvesting and oil extraction but the technology is progressing. Extensive research was conducted to determine the utilization of microalgae as an energy source and make algae oil production commercially viable. PMID:22586908

  9. Advances in biofuels

    CERN Document Server

    Ravindra, Pogaku

    2013-01-01

    This book examines the current technology used for extracting fuel from biomass for a variety of plant types. It explores shortcomings of each and details improved techniques, which would be cheaper, more efficient, and produce less waste.

  10. Advances in photobioreactors for intensive microalgal production: configurations, operating strategies and applications

    NARCIS (Netherlands)

    Olivieri, G.; Salatino, P.; Marzocchella, A.

    2014-01-01

    Over the past ten years a great deal of literature has focused on the biotechnological potential of microalgal commercial applications, mainly in the field of biofuel production. However, the biofuel production is not yet competitive, mainly due to the incidence of the photobioreactor technology on

  11. Novel biofuel formulations for enhanced vehicle performance

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Dennis [Michigan State Univ., East Lansing, MI (United States); Narayan, Ramani [Michigan State Univ., East Lansing, MI (United States); Berglund, Kris [Michigan State Univ., East Lansing, MI (United States); Lira, Carl [Michigan State Univ., East Lansing, MI (United States); Schock, Harold [Michigan State Univ., East Lansing, MI (United States); Jaberi, Farhad [Michigan State Univ., East Lansing, MI (United States); Lee, Tonghun [Michigan State Univ., East Lansing, MI (United States); Anderson, James [Michigan State Univ., East Lansing, MI (United States); Wallington, Timothy [Michigan State Univ., East Lansing, MI (United States); Kurtz, Eric [Michigan State Univ., East Lansing, MI (United States); Ruona, Will; Hass, Heinz

    2013-08-30

    engine under highly instrumented conditions. Simulation of and experimentation on combustion in single and multicylinder engines was carried out in detail throughout the project. The combustion behavior of biofuel blends neat and in petroleum were characterized in the MSU optical engine, in part to validate results obtained in the RCM and to provide data for comparison with simulations. Simulation of in- cylinder, low-temperature combustion included development of an extensive fuel injection model that included fuel spray breakup, evaporation, and ignition, along with prediction of cylinder temperature, pressure, and work produced. Single cylinder and multicylinder engine tests under advanced low-temperature combustion conditions conducted at Ford Motor Company validated experimental and simulation results obtained in the MSU engine and in MSU simulations. Single cylinder engine tests of an advanced biofuel containing biodiesel and dibutyl succinate, carried out under low-temperature combustion conditions, showed similar power generation and gas-phase emissions (CO, HC, NOx), but a reduction in particulates of as much as 60% relative to neat biodiesel and 95% relative to petroleum diesel at the same operating conditions. This remarkable finding suggests that biofuels may be able to play a role in eliminating the need for particulate removal systems in diesel vehicles. The multicylinder engine tests at Ford, carried out using butyl nonanoate as an advanced biofuel, also gave promising results, showing a strong decline in particulate emissions and simultaneously a modest decrease in NOx emissions relative to standard petroleum diesel at the same conditions. In summary, this project has shown that advanced biofuels and their blends are capable of maintaining performance while reducing emissions, particularly particulates (soot), in 3 compression ignition engines. The interdisciplinary nature of biofuel production and testing has identified fuel properties that are capable

  12. Water for Food, Energy, and the Environment: Assessing Streamflow Impacts of Increasing Cellulosic Biofuel Crop Production in the Corn Belt

    Science.gov (United States)

    Yaeger, M. A.; Housh, M.; Ng, T.; Cai, X.; Sivapalan, M.

    2012-12-01

    The recently expanded Renewable Fuel Standard, which now requires 36 billion gallons of renewable fuels by 2022, has increased demand for biofuel refinery feedstocks. Currently, biofuel production consists mainly of corn-based ethanol, but concern over increasing nitrate levels resulting from increased corn crop fertilization has prompted research into alternative biofuel feedstocks. Of these, high-yielding biomass crops such as Miscanthus have been suggested for cellulose-based ethanol production. Because these perennial crops require less fertilization and do not need tilling, increasing land area in the Midwest planted with Miscanthus would result in less nitrate pollution to the Gulf of Mexico. There is a tradeoff, however, as Miscanthus also has higher water requirements than conventional crops in the region. This could pose a serious problem for riparian ecosystems and other streamflow users such as municipalities and biofuel refineries themselves, as the lowest natural flows in this region coincide with the peak of the growing season. Moreover, low flow reduction may eventually cut off the water quality benefit that planting Miscanthus provides. Therefore, for large-scale cellulosic ethanol production to be sustainable, it is important to understand how the watershed will respond to this change in land and water use. To this end a detailed data analysis of current watershed conditions has been combined with hydrologic modeling to gain deeper insights into how catchments in the highly agricultural central IL watershed of the Sangamon River respond to current and future land and water usage, with the focus on the summer low-flow season. In addition, an integrated systems optimization model has been developed that combines hydrologic, agro-biologic, engineering infrastructural, and economic inputs to provide optimal scenarios of crop type and area and corresponding refinery locations and capacities. Through this integrated modeling framework, we address the key

  13. Biofuel initiatives in Japan: Strategies, policies, and future potential

    International Nuclear Information System (INIS)

    Japan has developed a variety of national strategies and plans related to biofuels which address four main policy objectives, including reduction of greenhouse gas (GHG) emissions, energy security, rural development, and realisation of a recycle-based society. This paper reviews these national strategies and plans as well as associated implementing policies, and discusses the extent to which these objectives may be achieved. This paper found that the long-term potential of biofuels to contribute to GHG reduction goals will depend not only on the rates of technological development of the second generation biofuels but also on the development of other advanced vehicles. In the medium term, the potential contribution of biofuels to rural development and realising a recycle-based society could become significant depending on the progress of technology for both second generation biofuel production and the collection and transportation of their feedstocks. The potential contribution of biofuels to Japan's energy security is constrained by the availability of imports and the potential of domestic production.

  14. PHYCOREMEDIATION AND THE POTENTIAL OF SUSTAINABLE ALGAL BIOFUEL PRODUCTION USING WASTEWATER

    Directory of Open Access Journals (Sweden)

    Adel A. Fathi

    2013-01-01

    Full Text Available Wastewater remediation by microalgae is an eco-friendly process with no secondary pollution as long as the biomass produced is reused and allows efficient nutrient recycling. This study was undertaken to evaluate the remediation of wastewater by the green alga Chlorella vulgaris and the potential of these alga (biomass to produce sustainable biofuel. The results shows that the cultivation of green alga Chlorella vulgaris on wastewater has a positive effect on removal the major inorganic elements form the wastewater. Biodiesel can be produced from Chlorella vulgaris. The Chlorella vulgaris can be used for remediation of wastewater and the producing biomass can be used as source of renewable energy.

  15. Synthetic Biology and Its Applications in Next-Generation Advanced Biofuels%面向先进生物燃料的合成生物学

    Institute of Scientific and Technical Information of China (English)

    刘斌; 陈方; 陈云伟; 丁陈君; 邓勇

    2012-01-01

    先进生物燃料一般指来自于非粮食原料的交通运输用生物燃料.近年来,先进生物燃料的发展引起了众多国家的浓厚兴趣,然而,先进生物燃料正处于关键的技术研发阶段,还需经过大量研发以突破技术障碍和示范生产活动后方能进行商业化部署.过去10年内,合成生物学研究大量兴起并不断取得突破,使人们有可能人工设计构建新的高效生命系统,克服生物燃料发展的技术瓶颈,进行先进生物燃料的生产.在介绍先进生物燃料与合成生物学的发展现状的基础上,分析了合成生物学在先进生物燃料研发中的重要价值与研发进展,探讨了合成生物学的发展潜力.%Advanced biofuels are high-energy liquid transportation fuels derived from sustainable biomass feedstocks including algae, which has attracted attention during the past few years as renewable and environmentally friendly alternative fuels, but many obstacles still remain in technical development and demonstration. In recent years, there has been a lot of breakthrough and progress in synthetic biology area, and therefore synthetic biological research has shown promising prospects in many areas, especially in that of advanced biofuels. The development of advanced biofuels and synthetic biology and its role in biofuels research and development is thoroughly demonstrated, and the opportunities and challenges of synthetic biology are also discussed.

  16. Advances in product family and product platform design methods & applications

    CERN Document Server

    Jiao, Jianxin; Siddique, Zahed; Hölttä-Otto, Katja

    2014-01-01

    Advances in Product Family and Product Platform Design: Methods & Applications highlights recent advances that have been made to support product family and product platform design and successful applications in industry. This book provides not only motivation for product family and product platform design—the “why” and “when” of platforming—but also methods and tools to support the design and development of families of products based on shared platforms—the “what”, “how”, and “where” of platforming. It begins with an overview of recent product family design research to introduce readers to the breadth of the topic and progresses to more detailed topics and design theory to help designers, engineers, and project managers plan, architect, and implement platform-based product development strategies in their companies. This book also: Presents state-of-the-art methods and tools for product family and product platform design Adopts an integrated, systems view on product family and pro...

  17. Production of a Biofuel that Keeps the Glycerol as a Monoglyceride by Using Supported KF as Heterogeneous Catalyst

    Directory of Open Access Journals (Sweden)

    Juan Calero

    2014-06-01

    Full Text Available This study describes the results obtained in the synthesis of a biofuel that avoids the production of glycerol by applying supported KF as alkaline heterogeneous catalyst, to generate two moles of fatty acid methyl esters and one mole of monoglyceride from one mol of triglyceride. In this respect, the selective transesterification process of sunflower oil with methanol was carried out with KF (10 wt% supported on three different solids, Al2O3, ZnO and MgO. The standard experimental conditions employed in the heterogeneous selective methanolysis reaction were: 12 mL of sunflower oil, 2.7 mL of methanol, 0.8 g of catalyst, at 65 °C temperature and one hour of reaction time. In all cases 100% conversion was obtained, with high selectivity values, greater than 90%, and quite suitable viscosity values, 4.5–8.5 cSt. In this way, the best catalytic behavior in the first use was obtained by using Al2O3 as support. However, although in the five consecutive reuses all catalysts exhibited a continuous decrease in their catalytic activities; the lower one was for KF catalyst using MgO as support. In summary, these three KF supported catalysts are very suitable to obtain a new biofuel, similar to conventional biodiesel, applicable to diesel engines.

  18. Advanced Algal Systems Fact Sheet

    Energy Technology Data Exchange (ETDEWEB)

    None

    2016-06-01

    Research and development (R&D) on advanced algal biofuels and bioproducts presents an opportunity to sustainably expand biomass resource potential in the United States. The Bioenergy Technologies Office’s (BETO’s) Advanced Algal Systems Program is carrying out a long-term, applied R&D strategy to lower the costs of algal biofuel production by working with partners to develop revolutionary technologies and conduct crosscutting analyses to better understand the potential

  19. Genetic modification of plant cell walls to enhance biomass yield and biofuel production in bioenergy crops.

    Science.gov (United States)

    Wang, Yanting; Fan, Chunfen; Hu, Huizhen; Li, Ying; Sun, Dan; Wang, Youmei; Peng, Liangcai

    2016-01-01

    Plant cell walls represent an enormous biomass resource for the generation of biofuels and chemicals. As lignocellulose property principally determines biomass recalcitrance, the genetic modification of plant cell walls has been posed as a powerful solution. Here, we review recent progress in understanding the effects of distinct cell wall polymers (cellulose, hemicelluloses, lignin, pectin, wall proteins) on the enzymatic digestibility of biomass under various physical and chemical pretreatments in herbaceous grasses, major agronomic crops and fast-growing trees. We also compare the main factors of wall polymer features, including cellulose crystallinity (CrI), hemicellulosic Xyl/Ara ratio, monolignol proportion and uronic acid level. Furthermore, the review presents the main gene candidates, such as CesA, GH9, GH10, GT61, GT43 etc., for potential genetic cell wall modification towards enhancing both biomass yield and enzymatic saccharification in genetic mutants and transgenic plants. Regarding cell wall modification, it proposes a novel groove-like cell wall model that highlights to increase amorphous regions (density and depth) of the native cellulose microfibrils, providing a general strategy for bioenergy crop breeding and biofuel processing technology. PMID:27269671

  20. Environmental and Social Impacts of Oil Palm Plantations and their Implications for Biofuel Production in Indonesia

    Directory of Open Access Journals (Sweden)

    Heru Komarudin

    2012-03-01

    Full Text Available This paper reviews the development of oil palm with linkages to biofuel in Indonesia and analyzes the associated environmental and socioeconomic impacts. We selected three plantation study sites in West Papua (Manokwari, West Kalimantan (Kubu Raya, and Papua (Boven Digoel to assess the impacts. Research findings indicate that the development of oil palm in all three sites has caused deforestation, resulting in significant secondary external impacts such as water pollution, soil erosion, and air pollution. In terms of social impacts, many stakeholder groups, i.e., employees, out-growers, and investing households, report significant gains. However, we found these benefits were not evenly distributed. Other stakeholders, particularly traditional landowners, experienced restrictions on traditional land use rights and land losses. We observed increasing land scarcity, rising land prices, and conflicts over land in all sites. Three major trade-offs are associated with the development of oil palm plantations, including those related to biofuels: unevenly distributed economic benefits are generated at the cost of significant environmental losses; there are some winners but also many losers; and economic gains accrue at the expense of weak rule of law. To reduce the negative impacts and trade-offs of oil palm plantations and maximize their economic potential, government decision makers need to restrict the use of forested land for plantation development, enforce existing regulations on concession allocation and environmental management, improve monitoring of labor practices, recognize traditional land use rights, and make land transfer agreements involving customary land more transparent and legally binding.

  1. Genetic modification of plant cell walls to enhance biomass yield and biofuel production in bioenergy crops.

    Science.gov (United States)

    Wang, Yanting; Fan, Chunfen; Hu, Huizhen; Li, Ying; Sun, Dan; Wang, Youmei; Peng, Liangcai

    2016-01-01

    Plant cell walls represent an enormous biomass resource for the generation of biofuels and chemicals. As lignocellulose property principally determines biomass recalcitrance, the genetic modification of plant cell walls has been posed as a powerful solution. Here, we review recent progress in understanding the effects of distinct cell wall polymers (cellulose, hemicelluloses, lignin, pectin, wall proteins) on the enzymatic digestibility of biomass under various physical and chemical pretreatments in herbaceous grasses, major agronomic crops and fast-growing trees. We also compare the main factors of wall polymer features, including cellulose crystallinity (CrI), hemicellulosic Xyl/Ara ratio, monolignol proportion and uronic acid level. Furthermore, the review presents the main gene candidates, such as CesA, GH9, GH10, GT61, GT43 etc., for potential genetic cell wall modification towards enhancing both biomass yield and enzymatic saccharification in genetic mutants and transgenic plants. Regarding cell wall modification, it proposes a novel groove-like cell wall model that highlights to increase amorphous regions (density and depth) of the native cellulose microfibrils, providing a general strategy for bioenergy crop breeding and biofuel processing technology.

  2. Exergy analysis of synthetic biofuel production via fast pyrolysis and hydroupgrading

    International Nuclear Information System (INIS)

    This paper presents the first assessment of the exergetic performance of a biorefinery process based on catalytic hydroupgrading of bio-oil from fast pyrolysis. Lignocellulosic biomass is converted into bio-oil through fast pyrolysis, which is then upgraded to synthetic fuels in a catalytic hydrotreating process. The biorefinery process is simulated numerically using commercial software and analyzed using exergetic analysis. Exergy balances are defined for each component of the plant and the exergetic efficiencies and exergy destruction rates are calculated at the component, section and plant level, identifying thermodynamic inefficiencies and revealing the potential for further improvement of the process. The overall biofuel process results in an exergetic efficiency of 60.1%, while the exergetic efficiency of the upgrading process in the biorefinery alone is 77.7%. Within the biorefinery, the steam reforming reactor is the main source of inefficiencies, followed by the two hydrotreating reactors. In spite of the high operating pressures in the hydrotreating section, the compressors have little impact on the total exergy destruction. Compared to competing lignocellulosic biofuel processes, like gasification with Fischer–Tropsch synthesis or lignocellulosic ethanol processes, the examined system achieves a significantly higher exergetic efficiency. - Highlights: • Exergetic analysis of a biorefinery for bio-oil hydroupgrading. • Detailed simulation model using 83 model compounds. • Exergy destruction quantified in each component of the plant. • Exergetic efficiency and potential for improvement determined on component level. • Highest exergy destruction in the pyrolysis plant and the steam reformer

  3. Screening of natural yeast isolates under the effects of stresses associated with second-generation biofuel production.

    Science.gov (United States)

    Dubey, Rajni; Jakeer, Shaik; Gaur, Naseem A

    2016-05-01

    Robust microorganisms are required for sustainable second-generation biofuel production. We evaluated the growth and fermentation performance of six natural isolates that were derived from grape wine and medicinal herbs using a wide range of carbon sources, rice and wheat straw hydrolysates as well as stress conditions associated with second-generation ethanol production. Sequence analysis of the 5.8S internal transcribed spacer (ITS) and species-specific PCR amplification of the HO gene region assigned the natural isolates to Saccharomyces cerevisiae. Restriction fragment length polymorphism (RFLP) analysis of the mitochondrial DNA revealed that natural yeast isolates are genetically closer to the laboratory strain BY4741 than to the CEN.PK strains. Dextrose fermentation by a natural isolate, MTCC4780, under semi-anaerobic conditions produced maximum ethanol yields of 0.44 g/g and 0.39 g/g, respectively, with and without the stresses encountered during lignocellulosic ethanol fermentation. However, MTCC4780 produced ethanol yields of 0.48 g/g, 0.42 g/g and 0.45 g/g, respectively, with glucose, rice and wheat straw enzymatic hydrolysate fermentation in a bioreactor. The isolates MTCC4781 and MTCC4796 showed higher growth and fermentation performance than did MTCC4780 in the presence of elevated temperature and pre-treatment inhibitors. Taken together, the MTCC4780, MTCC4781 and MTCC4796 strains have the potential to serve as a platform for lignocellulosic ethanol production under stresses associated with second-generation biofuel production.

  4. Screening of natural yeast isolates under the effects of stresses associated with second-generation biofuel production.

    Science.gov (United States)

    Dubey, Rajni; Jakeer, Shaik; Gaur, Naseem A

    2016-05-01

    Robust microorganisms are required for sustainable second-generation biofuel production. We evaluated the growth and fermentation performance of six natural isolates that were derived from grape wine and medicinal herbs using a wide range of carbon sources, rice and wheat straw hydrolysates as well as stress conditions associated with second-generation ethanol production. Sequence analysis of the 5.8S internal transcribed spacer (ITS) and species-specific PCR amplification of the HO gene region assigned the natural isolates to Saccharomyces cerevisiae. Restriction fragment length polymorphism (RFLP) analysis of the mitochondrial DNA revealed that natural yeast isolates are genetically closer to the laboratory strain BY4741 than to the CEN.PK strains. Dextrose fermentation by a natural isolate, MTCC4780, under semi-anaerobic conditions produced maximum ethanol yields of 0.44 g/g and 0.39 g/g, respectively, with and without the stresses encountered during lignocellulosic ethanol fermentation. However, MTCC4780 produced ethanol yields of 0.48 g/g, 0.42 g/g and 0.45 g/g, respectively, with glucose, rice and wheat straw enzymatic hydrolysate fermentation in a bioreactor. The isolates MTCC4781 and MTCC4796 showed higher growth and fermentation performance than did MTCC4780 in the presence of elevated temperature and pre-treatment inhibitors. Taken together, the MTCC4780, MTCC4781 and MTCC4796 strains have the potential to serve as a platform for lignocellulosic ethanol production under stresses associated with second-generation biofuel production. PMID:26481160

  5. White paper report from working groups attending the international conference on research and educational opportunities in bio-fuel crop production

    Energy Technology Data Exchange (ETDEWEB)

    Morgan, K.T. [University of Florida, Soil and Water Science Dep., Southwest Florida Res. and Educ. Center, Immokalee, FL 34142 (United States); Gilbert, R.A. [University of Florida, Agronomy Dep., Everglades Res. and Educ. Center, Belle Glade, FL 33430 (United States); Helsel, Z.A. [Rutgers University, Plant Biology and Pathology Dep., New Brunswick, NJ 08901-8520 (United States); Buacum, L. [University of Florida, Hendry County Extension, LaBelle, FL 33935 (United States); Leon, R.; Perret, J. [EARTH University, Apto. 4442-1000, San Jose (Costa Rica)

    2010-12-15

    A conference on current research and educational programs in production of crops for bio-fuel was sponsored and organized by the EARTH University and the University of Florida in November, 2008. The meeting addressed current research on crops for bio-fuel production with discussions of research alternatives for future crop production systems, land use issues, ethics of food vs. fuel production, and carbon sequestration in environmentally sensitive tropical and sub-tropical regions of the Americas. The need and potential for development of graduate and undergraduate curricula and inter-institutional cooperation among educational institutions in the region were also discussed. Delegations from Belize, Brazil, Columbia, Costa Rica, Cuba, Honduras, Panama, The Dominican Republic, and the United States including ministers of Agriculture and Energy attended this meeting. Over a two-day period, four working groups provided a framework to facilitate networking, motivate task oriented creative thinking, and maintain a timely accomplishment of assigned duties in the context of the conference themes. Participants in the conference were assigned to one of four working groups, each following given topics: Agronomy, Environment, Socio-Economics and Education/Extension. It was the consensus of representatives of industry, academic and regulatory community assembled in Costa Rica that significant research, education and socio-economic information is needed to make production of bio-fuel crops sustainable. Agronomic research should include better crop selection based on local conditions, improved production techniques, pest and disease management, and mechanical cultivation and harvesting. Another conclusion was that tailoring of production systems to local soil characteristics and use of bio-fuel by-products to improve nutrient use efficiency and reduction of environmental impact on water quantity and quality is critical to sustainability of bio-fuel crop production. (author)

  6. Water Management Applications of Advanced Precipitation Products

    Science.gov (United States)

    Johnson, L. E.; Braswell, G.; Delaney, C.

    2012-12-01

    Advanced precipitation sensors and numerical models track storms as they occur and forecast the likelihood of heavy rain for time frames ranging from 1 to 8 hours, 1 day, and extended outlooks out to 3 to 7 days. Forecast skill decreases at the extended time frames but the outlooks have been shown to provide "situational awareness" which aids in preparation for flood mitigation and water supply operations. In California the California-Nevada River Forecast Centers and local Weather Forecast Offices provide precipitation products that are widely used to support water management and flood response activities of various kinds. The Hydrometeorology Testbed (HMT) program is being conducted to help advance the science of precipitation tracking and forecasting in support of the NWS. HMT high-resolution products have found applications for other non-federal water management activities as well. This presentation will describe water management applications of HMT advanced precipitation products, and characterization of benefits expected to accrue. Two case examples will be highlighted, 1) reservoir operations for flood control and water supply, and 2) urban stormwater management. Application of advanced precipitation products in support of reservoir operations is a focus of the Sonoma County Water Agency. Examples include: a) interfacing the high-resolution QPE products with a distributed hydrologic model for the Russian-Napa watersheds, b) providing early warning of in-coming storms for flood preparedness and water supply storage operations. For the stormwater case, San Francisco wastewater engineers are developing a plan to deploy high resolution gap-filling radars looking off shore to obtain longer lead times on approaching storms. A 4 to 8 hour lead time would provide opportunity to optimize stormwater capture and treatment operations, and minimize combined sewer overflows into the Bay.ussian River distributed hydrologic model.

  7. Transporter-mediated biofuel secretion.

    Science.gov (United States)

    Doshi, Rupak; Nguyen, Tuan; Chang, Geoffrey

    2013-05-01

    Engineering microorganisms to produce biofuels is currently among the most promising strategies in renewable energy. However, harvesting these organisms for extracting biofuels is energy- and cost-intensive, limiting the commercial feasibility of large-scale production. Here, we demonstrate the use of a class of transport proteins of pharmacological interest to circumvent the need to harvest biomass during biofuel production. We show that membrane-embedded transporters, better known to efflux lipids and drugs, can be used to mediate the secretion of intracellularly synthesized model isoprenoid biofuel compounds to the extracellular milieu. Transporter-mediated biofuel secretion sustainably maintained an approximate three- to fivefold boost in biofuel production in our Escherichia coli test system. Because the transporters used in this study belong to the ubiquitous ATP-binding cassette protein family, we propose their use as "plug-and-play" biofuel-secreting systems in a variety of bacteria, cyanobacteria, diatoms, yeast, and algae used for biofuel production. This investigation showcases the potential of expressing desired membrane transport proteins in cell factories to achieve the export or import of substances of economic, environmental, or therapeutic importance.

  8. Regional Impacts of Miscanthus Biofuel Feedstock Production on the Hydrologic Cycle

    Science.gov (United States)

    Vanloocke, A. D.; Twine, T. E.; Bernacchi, C.

    2009-12-01

    Socio-economic and scientific interest toward the use of renewable energy to offset fossil fuel dependence and greenhouse gas emissions is increasing. Currently, the majority of the US renewable energy production is focused on replacing gasoline with corn ethanol. In 2008, 18% of the US corn yield was used to displace ~5% of US gasoline consumption. This represents progress toward meeting the goals of offsetting 30% of liquid fossil fuel consumption by 2030 as established by the US government in the Advanced Energy Initiative (AEI). However, a growing body of research indicates that it may not be beneficial or even possible for corn ethanol alone to meet the AEI goals. Highly productive bioenergy feedstocks requiring fewer inputs such as Miscanthus x Giganteus (Miscanthus) are ideal candidates, relative to maize, to provide a renewable and sustainable alternative to fossil fuel. It is anticipated that Miscanthus is likely to have minimal environmental impacts and could be potentially beneficial to the environment. In order to meet the AEI goals, Miscanthus production on the scale of 1x10 ha would be needed. Before this level of production occurs, uncertainty over the environmental impacts of large-scale implementation should be addressed particularly with regards to the hydrologic cycle. We calibrated and evaluated a process-based terrestrial ecosystem model, Agro-IBIS (Integrated Biosphere Simulator, agricultural version), to simulate the impacts of land-use-change from current land-use practices to Miscanthus production on the hydrologic cycle. Simulations for the Midwestern US (0.5°grid cell resolution) were generated using the same climate forcing for current land cover and additional scenarios where Miscanthus was planted in varying densities (10%, 25%, 50%, 75%, and 100%). Analyses indicate that for most of the Midwestern US, large increases in evapotranspiration (~100 to 250 mm/yr) and decreases in drainage (~ -100 to -250 mm/yr) occur when high densities

  9. Assessing the environmental sustainability of biofuels.

    Science.gov (United States)

    Kazamia, Elena; Smith, Alison G

    2014-10-01

    Biofuels vary in their potential to reduce greenhouse gas emissions when displacing fossil fuels. Savings depend primarily on the crop used for biofuel production, and on the effect that expanding its cultivation has on land use. Evidence-based policies should be used to ensure that maximal sustainability benefits result from the development of biofuels. PMID:25281367

  10. World Biofuels Study

    Energy Technology Data Exchange (ETDEWEB)

    Alfstad,T.

    2008-10-01

    This report forms part of a project entitled 'World Biofuels Study'. The objective is to study world biofuel markets and to examine the possible contribution that biofuel imports could make to help meet the Renewable Fuel Standard (RFS) of the Energy Independence and Security Act of 2007 (EISA). The study was sponsored by the Biomass Program of the Assistant Secretary for Energy Efficiency and Renewable Energy (EERE), U.S. Department of Energy. It is a collaborative effort among the Office of Policy and International Affairs (PI), Department of Energy and Oak Ridge National Laboratory (ORNL), National Renewable Energy Laboratory (NREL) and Brookhaven National Laboratory (BNL). The project consisted of three main components: (1) Assessment of the resource potential for biofuel feedstocks such as sugarcane, grains, soybean, palm oil and lignocellulosic crops and development of supply curves (ORNL). (2) Assessment of the cost and performance of biofuel production technologies (NREL). (3) Scenario-based analysis of world biofuel markets using the ETP global energy model with data developed in the first parts of the study (BNL). This report covers the modeling and analysis part of the project conducted by BNL in cooperation with PI. The Energy Technology Perspectives (ETP) energy system model was used as the analytical tool for this study. ETP is a 15 region global model designed using the MARKAL framework. MARKAL-based models are partial equilibrium models that incorporate a description of the physical energy system and provide a bottom-up approach to study the entire energy system. ETP was updated for this study with biomass resource data and biofuel production technology cost and performance data developed by ORNL and NREL under Tasks 1 and 2 of this project. Many countries around the world are embarking on ambitious biofuel policies through renewable fuel standards and economic incentives. As a result, the global biofuel demand is expected to grow very

  11. The potential of freshwater macroalgae as a biofuels feedstock and the influence of nutrient availability on freshwater macroalgal biomass production

    Science.gov (United States)

    Yun, Jin-Ho

    Extensive efforts have been made to evaluate the potential of microalgae as a biofuel feedstock during the past 4-5 decades. However, filamentous freshwater macroalgae have numerous characteristics that favor their potential use as an alternative algal feedstock for biofuels production. Freshwater macroalgae exhibit high rates of areal productivity, and their tendency to form dense floating mats on the water surface imply significant reductions in harvesting and dewater costs compared to microalgae. In Chapter 1, I reviewed the published literature on the elemental composition and energy content of five genera of freshwater macroalgae. This review suggested that freshwater macroalgae compare favorably with traditional bio-based energy sources, including terrestrial residues, wood, and coal. In addition, I performed a semi-continuous culture experiment using the common Chlorophyte genus Oedogonium to investigate whether nutrient availability can influence its higher heating value (HHV), productivity, and proximate analysis. The experimental study suggested that the most nutrient-limited growth conditions resulted in a significant increase in the HHV of the Oedogonium biomass (14.4 MJ/kg to 16.1 MJ/kg). Although there was no significant difference in productivity between the treatments, the average dry weight productivity of Oedogonium (3.37 g/m2/day) was found to be much higher than is achievable with common terrestrial plant crops. Although filamentous freshwater macroalgae, therefore, have significant potential as a renewable source of bioenergy, the ultimate success of freshwater macroalgae as a biofuel feedstock will depend upon the ability to produce biomass at the commercial-scale in a cost-effective and sustainable manner. Aquatic ecology can play an important role to achieve the scale-up of algal crop production by informing the supply rates of nutrients to the cultivation systems, and by helping to create adaptive production systems that are resilient to

  12. Microalgae for third generation biofuel production, mitigation of greenhouse gas emissions and wastewater treatment: Present and future perspectives – A mini review

    International Nuclear Information System (INIS)

    The extensive use of fossil fuels is increasingly recognized as unsustainable as a consequence of depletion of supplies and the contribution of these fuels to climate change by GHG (greenhouse gas) emissions into the atmosphere. Microalgae indicate alternative renewable sustainable energy sources as they have a high potential for producing large amounts of biomass which in turn can be used for production of different third-generation biofuels at large scale. Microalgae transform the solar energy into the carbon storage products, leads to lipid accumulation, including TAG (triacylglycerols), which then can be transformed into biodiesel, bioethanol and biomethanol. This paper reviews the selection, production and accumulation of target bioenergy carrier's strains and their advantages as well as the technological development for oil, biodiesel, ethanol, methanol, biogas production and GHG mitigation. The feedstock of promising algal strain exhibits the suitable biofuel production. The current progress of hybrid-technologies (biomass production, wastewater treatment, GHG mitigation) for production of prime-products as biofuels offer atmospheric pollution control such as the reduction of GHG (CO2 fixation) coupling wastewater treatment with microalgae growth. The selection of efficient strain, microbial metabolism, cultivation systems, biomass production are key parameters of viable technology for microalgae-based biodiesel-production. - Highlights: • Microalgae are promising feedstock for biofuel production within lower farming area. • Production rate (L/ha) of oil from microalgae is much higher than other feedstock. • Lipid of Chlorella emersonii, Botryococcus braunii, Dunaliella tertiolecta, are high (>60% of dw biomass). • Remove pollutant from wastewater during feedstock production by selective strains. • Ecofriendly route to mitigate GHG (greenhouse gas) and water pollution during microalgae production

  13. Biological nitrogen fixation in sugar cane: A key to energetically viable biofuel production

    Energy Technology Data Exchange (ETDEWEB)

    Boddey, R.M. [Centro Nacional de Pesquisa de Agrobiologia, Rio de Janeiro (Brazil)

    1995-05-01

    The advantages of producing biofuels to replace fossil energy sources are derived from the fact that the energy accumulated in the biomass in captured directly from photosynthesis and is thus renewable, and that the cycle of carbon dioxide fixation by the crop, followed by burning of the fuel makes no overall contribution to atmospheric CO{sub 2} or, consequently, to global warming. However, these advantages are negated if large quantities of fossil fuels need to be used to grow or process the biofuel crop. In this regard, the Brazilian bioethanol program, based on the fermentation/distillation of sugar cane juice, is particularly favorable, not only because the crop is principally hand harvested, but also because of the low nitrogen fertilizer use on sugar cane in Brazil. Recent {sup 15}N and N balance studies have shown that in some Brazilian cane varieties, high yields are possible without N fertilization because the plants are able to obtain large contributions of nitrogen from plant-associated biological N{sub 2} fixation (BNF). The N{sub 2}-fixing acid-tolerant bacterium Acetobacter diazotrophicus was first found to occur within roots, stems, and leaves of sugar cane. Subsequently, two species of Herbaspirillum also have been found to occur within the interior of all sugar cane tissues. The discovery of these, and other N{sub 2}-fixing bacteria that survive poorly in soil but thrive within plant tissue (endophytic bacteria), may account for the high BNF contributions observed in sugar cane. Further study of this system should allow the gradual elimination of N fertilizer use on sugar cane, at least in Brazil, and opens up the possibility of the extension of this efficient N{sub 2}-fixing system to cereal and other crops with consequent immense potential benefits to tropical agriculture. 44 refs., 9 figs., 4 tabs.

  14. Development of synthetic chromosomes and improved microbial strains to utilize cellulosic feedstocks and express valuable coproducts for sustainable production of biofuels from corn

    Science.gov (United States)

    A sustainable biorefinery must convert a broad range of renewable feedstocks into a variety of product streams, including fuels, power, and value-added bioproducts. To accomplish this, microbial-based technologies that enable new commercially viable coproducts from corn-to-ethanol biofuel fermentati...

  15. Use of biofuel by-product from the green algae Desmochloris sp. and diatom Nanofrustulum sp. meal in diets for nile tilapia Oreochromis niloticus

    Science.gov (United States)

    Algal by-product meals from the Hawaiian biofuels industry were evaluated as protein ingredients in diets for juveniles of Nile tilapia (Oreochromis niloticus). Four experimental diets were formulated to contain 40% protein and were made with fish meal, soybean meal, whole diatom (Nanofrustulum sp.)...

  16. A NOVEL STRAIN OF Aspergillus niger PRODUCING A COCKTAIL OF HYDROLYTIC DEPOLYMERISING ENZYMES FOR THE PRODUCTION OF SECOND GENERATION BIOFUELS

    Directory of Open Access Journals (Sweden)

    Namita Bansal

    2011-02-01

    Full Text Available The screening and isolation of fungi producing a cocktail of hydrolytic enzymes was studied. Among the various isolates obtained from different soil samples, a strain NS-2 was selected. The phylogenetic analysis of this strain showed highest homology (99% with Aspergillus niger. It was capable of producing cellulolytic, hemicellulolytic, amylolytic, and pectinolytic enzymes in appreciable titers on wheat bran based liquid and solid state media. The mixture of enzymes produced by this organism could effectively hydrolyze various domestic waste residues, revealing conversion efficiencies of 89 to 92% and produced high reducing sugar yields of 0.48 to 0.66 g/g of dry residue. This enzyme cocktail could potentially find a significant application in the conversion of agricultural and other waste residues having cellulose, hemicellulose, starch, and pectin as carbohydrates to produce simpler sugars which can be fermented for the production of second generation biofuels.

  17. Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization.

    Science.gov (United States)

    Whitaker, William B; Sandoval, Nicholas R; Bennett, Robert K; Fast, Alan G; Papoutsakis, Eleftherios T

    2015-06-01

    Synthetic methylotrophy is the development of non-native methylotrophs that can utilize methane and methanol as sole carbon and energy sources or as co-substrates with carbohydrates to produce metabolites as biofuels and chemicals. The availability of methane (from natural gas) and its oxidation product, methanol, has been increasing, while prices have been decreasing, thus rendering them as attractive fermentation substrates. As they are more reduced than most carbohydrates, methane and methanol, as co-substrates, can enhance the yields of biologically produced metabolites. Here we discuss synthetic biology and metabolic engineering strategies based on the native biology of aerobic methylotrophs for developing synthetic strains grown on methanol, with Escherichia coli as the prototype. PMID:25796071

  18. National Algal Biofuels Technology Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    Ferrell, John [Dept. of Energy (DOE), Washington DC (United States); Sarisky-Reed, Valerie [Dept. of Energy (DOE), Washington DC (United States)

    2010-05-01

    The framework for National Algal Biofuels Technology Roadmap was constructed at the Algal Biofuels Technology Roadmap Workshop, held December 9-10, 2008, at the University of Maryland-College Park. The Workshop was organized by the Biomass Program to discuss and identify the critical challenges currently hindering the development of a domestic, commercial-scale algal biofuels industry. This Roadmap presents information from a scientific, economic, and policy perspectives that can support and guide RD&D investment in algal biofuels. While addressing the potential economic and environmental benefits of using algal biomass for the production of liquid transportation fuels, the Roadmap describes the current status of algae RD&D. In doing so, it lays the groundwork for identifying challenges that likely need to be overcome for algal biomass to be used in the production of economically viable biofuels.

  19. International Trade of Biofuels (Brochure)

    Energy Technology Data Exchange (ETDEWEB)

    2013-05-01

    In recent years, the production and trade of biofuels has increased to meet global demand for renewable fuels. Ethanol and biodiesel contribute much of this trade because they are the most established biofuels. Their growth has been aided through a variety of policies, especially in the European Union, Brazil, and the United States, but ethanol trade and production have faced more targeted policies and tariffs than biodiesel. This fact sheet contains a summary of the trade of biofuels among nations, including historical data on production, consumption, and trade.

  20. Effects of fatty acid activation on photosynthetic production of fatty acid-based biofuels in Synechocystis sp. PCC6803

    Directory of Open Access Journals (Sweden)

    Gao Qianqian

    2012-03-01

    Full Text Available Abstract Background Direct conversion of solar energy and carbon dioxide to drop in fuel molecules in a single biological system can be achieved from fatty acid-based biofuels such as fatty alcohols and alkanes. These molecules have similar properties to fossil fuels but can be produced by photosynthetic cyanobacteria. Results Synechocystis sp. PCC6803 mutant strains containing either overexpression or deletion of the slr1609 gene, which encodes an acyl-ACP synthetase (AAS, have been constructed. The complete segregation and deletion in all mutant strains was confirmed by PCR analysis. Blocking fatty acid activation by deleting slr1609 gene in wild-type Synechocystis sp. PCC6803 led to a doubling of the amount of free fatty acids and a decrease of alkane production by up to 90 percent. Overexpression of slr1609 gene in the wild-type Synechocystis sp. PCC6803 had no effect on the production of either free fatty acids or alkanes. Overexpression or deletion of slr1609 gene in the Synechocystis sp. PCC6803 mutant strain with the capability of making fatty alcohols by genetically introducing fatty acyl-CoA reductase respectively enhanced or reduced fatty alcohol production by 60 percent. Conclusions Fatty acid activation functionalized by the slr1609 gene is metabolically crucial for biosynthesis of fatty acid derivatives in Synechocystis sp. PCC6803. It is necessary but not sufficient for efficient production of alkanes. Fatty alcohol production can be significantly improved by the overexpression of slr1609 gene.