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Sample records for adenomyosis tissue injury

  1. Transcriptome analysis of endometrial tissues following GnRH agonist treatment in a mouse adenomyosis model

    Science.gov (United States)

    Guo, Song; Lu, Xiaowei; Gu, Ruihuan; Zhang, Di; Sun, Yijuan; Feng, Yun

    2017-01-01

    Purpose Adenomyosis is a common, benign gynecological condition of the female reproductive tract characterized by heavy menstrual bleeding and dysmenorrhea. Gonadotropin-releasing hormone (GnRH) agonists are one of the medications used in adenomyosis treatment; however, their underlying mechanisms are poorly understood. Moreover, it is difficult to obtain endometrial samples from women undergoing such treatment. To overcome this, we generated an adenomyosis mouse model, which we treated with an GnRH agonist to determine its effect on pregnancy outcomes. We also analyzed endometrial gene expression following GnRH agonist treatment to determine the mechanisms that may affect pregnancy outcome in individuals with adenomyosis. Methods Neonatal female mice were divided into a control group, an untreated adenomyosis group, and an adenomyosis group treated with a GnRH agonist (n=6 each). The pregnancy outcome was observed and compared among the groups. Then, three randomly chosen transcriptomes from endometrial tissues from day 4 of pregnancy were analyzed between the adenomyosis group and the GnRH agonist treatment group by RNA sequencing and quantitative reverse transcription polymerase chain reaction (PCR). Results The litter size was significantly smaller in the adenomyosis group than in the control group (7±0.28 vs 11±0.26; Ppregnancy outcome of adenomyosis in a mouse model. Besides pituitary down-regulation, other possible mechanisms such as the regulation of cell proliferation may play a role in this. These new insights into GnRH agonist mechanisms will be useful for future adenomyosis treatment. PMID:28331289

  2. Canine Uterine Leiomyoma with Epithelial Tissue Foci, Adenomyosis, and Cystic Endometrial Hyperplasia

    Directory of Open Access Journals (Sweden)

    George S. Karagiannis

    2011-01-01

    Full Text Available An 11-year-old Labrador Retriever bitch with a history of intermittent, sanguineous vaginal discharge of a six-month duration was presented. During exploratory laparotomy, two well-delineated, intramural masses were identified bilaterally in the uterine horns. Histopathologic examination of the mass on the left horn showed that it was a typical leiomyoma. However, the second mass appeared with an unusual coexistence of histological lesions, involving epithelial tissue foci, mild focal adenomyosis, and cystic endometrial hyperplasia. Interestingly, such combination was never encountered before in dogs. Although uterine leiomyoma is quite usual in the reproductive system of female dogs, this case resembled relevant cases of human uterine adenomyomas in morphology, and thus it was offered a similar tentative diagnosis.

  3. Is adenomyosis an immune disease?

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    Ota, H; Igarashi, S; Hatazawa, J; Tanaka, T

    1998-01-01

    Adenomyosis is characterized as ectopic endometrial tissues within the myometrium in the uterus. The only difference between adenomyosis and endometriosis is the site of endometriotic tissues: inside or outside of the uterus. It is well known that endometriosis is frequently associated with various autoimmune phenomena. This short review covers various aspects of the immune cascade found in adenomyosis. In adenomyosis, a series of immune responses is activated, including changes in both cellular and humoral immunity, i.e. a strong expression of cell surface antigens or adhesion molecules, an increased number of macrophages or immune cells, and deposition of immunoglobulins and complement components. Furthermore, the disease exhibited high frequency of autoantibodies in peripheral blood. Thus, an immunological 'vicious circle' is formed in the endometrium in adenomyosis. Endometrial cells seem to be under immunological stress, protecting themselves by exposing heat shock proteins. It is concluded that the endometrial environment in adenomyosis differs widely from that in normal fertile women. These abnormal immune responses might be involved in poor reproductive performance in adenomyosis.

  4. Fluorescence diagnosis in tissue injury

    Science.gov (United States)

    Maciel, Vitória H.; Ferreira, Juliana; Bagnato, Vanderlei S.

    2009-06-01

    Background and Objectives: The paper aim was to evaluate the efficacy of the fluorescence spectroscopy in the detection of UV-induced skin change of Wistar rats. Study Design/ Materials and Methods: In a group male Wistar rats, the skin damage was produced by an UV-C lamp, periodically monitored using the laser-induced fluorescence, until complete healing process. After determining a characteristic emission band present in the fluorescence spectra of the induced injuries, the amplitude band monitoring allowed the follow up on the injury and the recovery. Results: We observed the appearance of two new emission bands more evident at the injury spectra when compared to the spectrums from normal non-exposed tissue. Following such spectral bands was possible to observe the establishment and recovery. Conclusions: The fluorescence spectroscopy is a promising technique in distinguishing between normal and UV induced skin change helping the evaluation of changes which are irreversible cancer tissue characteristics.

  5. A Case of Adenomyosis with a High Titer of IgG Autoantibody to Calreticulin

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    Neil M. Gude BSc, MSc, PhD

    2013-10-01

    Full Text Available Background. High prevalence of autoantibodies to the calcium-binding, endoplasmic reticulum chaperone protein calreticulin has been reported in various autoimmune and parasitic diseases. It has been reported that adenomyosis is associated with the presence of autoantibodies, in particular to phospholipids; however, it is not known whether it is associated with autoimmunity to calreticulin. Results. A 35-year-old gravida 4 para 4 woman presented with a history of many years of intractable menorrhagia. Histopathological examination of a subsequent hysterectomy specimen revealed a bulky uterus, a poorly developed secretory endometrium with decidualization of the stroma and chronic endometritis, as well as the presence of adenomyosis uteri. IgG autoantibodies to calreticulin were measured in the plasma of this and 234 other patients. Nine (3.8% patients tested positive. The titer of anticalreticulin IgG autoantibody in the sole case with adenomyosis was approximately 8 times the average of other positive-testing samples. Conclusions. The etiology of adenomyosis is unclear. The presence of a high titer, blocking anticalreticulin autoantibody may directly increase the risk that adenomyosis might develop. It is also possible that the expansion of endometrial glandular tissue, as well as elevated estrogens, during adenomyosis may lead to elevated calreticulin, which induces an autoimmune reaction to it. Further study is required to determine whether there is a significant association between adenomyosis and the prevalence of calreticulin autoantibodies.

  6. Effects of LNG-IUS on nerve growth factor and its receptors expression in patients with adenomyosis.

    Science.gov (United States)

    Choi, Young Sik; Cho, Sihyun; Lim, Kyung Jin; Jeon, Young Eun; Yang, Hyo In; Lee, Kyung Eun; Heena, Kamdar; Seo, Seok Kyo; Kim, Hye Yeon; Lee, Byung Seok

    2010-12-01

    The levonorgestrel-releasing intrauterine system (LNG-IUS) is effective in the treatment of dysmenorrhea associated with adenomyosis. However, the mechanism of pain relief of LNG-IUS in patients with adenomyosis is unclear. We aimed to investigate the effects of LNG-IUS on the expression of nerve growth factor (NGF) and its receptors, NGFR p75 and TrkA in patients with adenomyosis. Endometrial and myometrial tissues were prepared from 17 LNG-IUS-treated patients and 15 hormonally untreated patients who had undergone hysterectomies for adenomyosis. Immunohistochemistry with antibodies against NGF, NGFR p75, and TrkA, was performed. The expression of NGF, NGFR p75, and TrkA in endometrium and myometrium of LNG-IUS-treated patients was significantly decreased compared to those of hormonally untreated patients. Our findings may indicate that the suppression of NGF and its receptors by LNG-IUS is another possible mechanism of relieving pain in patients with adenomyosis.

  7. Adenomyosis and its impact on women fertility

    Directory of Open Access Journals (Sweden)

    Elisabetta Garavaglia

    2015-06-01

    Full Text Available Adenomyosis is a widespread disease affecting the reproductive period of women’s life. In the last ten years, different pathogenetic hypotheses have been proposed to explain the initiation and development of the disease. This article aims to present and discuss the most important pathophysiologic mechanisms underlying adenomyosis development in order to clarify the relationship between adenomyosis and infertility. A PubMed search was undertaken for English language literature using the MeSH terms ‘adenomyosis’, ‘infertility’, ‘treatment’, and ‘pathogenesis’. Although the exact etiology of adenomyosis is unknown, many theories have been proposed. We analysed the most important pathogenic theories expressed and evaluated the potential consequences on women fertility. A better comprehension of the adenomyosis pathogenesis has allowed realizing that adenomyosis may affect young women and may have a great impact on their fertility through different mechanisms. The understanding of these mechanisms helps to clarify the potential usefulness of current therapies.

  8. Adenomyosis: usual and unusual imaging manifestations, pitfalls, and problem-solving MR imaging techniques.

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    Takeuchi, Mayumi; Matsuzaki, Kenji

    2011-01-01

    Adenomyosis is a common nonneoplastic gynecologic disease characterized by the presence of ectopic endometrium within the myometrium. On T2-weighted magnetic resonance (MR) images, typical adenomyosis appears as an ill-demarcated low-signal-intensity lesion with uterine enlargement. However, various physiologic or pathologic states such as amount of functional endometrial tissue, phase of the menstrual cycle, endogenous hormonal abnormality, and exogenous hormonal stimulation may affect the MR imaging appearance of adenomyosis and may result in a tumorlike appearance. Problem-solving MR imaging techniques used in diagnosis of adenomyosis include diffusion-weighted imaging, susceptibility-weighted imaging, hydrogen 1 MR spectroscopy, cine MR imaging, and high-resolution MR imaging at 3 T. Adenomyotic lesions that show high signal intensity relative to the outer myometrium on T2-weighted images mimic malignancies such as leiomyosarcoma and endometrial stromal sarcoma. In these cases, a relatively high apparent diffusion coefficient at diffusion-weighted imaging and a low choline peak at MR spectroscopy are suggestive of a benign lesion. Small hemorrhagic foci suggestive of an adenomyotic lesion are well demonstrated as signal voids at susceptibility-weighted imaging. Cine MR imaging is useful in differentiating transient myometrial contraction from focal adenomyosis. High-resolution MR imaging at 3 T demonstrates anatomically detailed structures and may improve diagnostic accuracy in differentiating adenomyosis from its mimics, such as low-grade endometrial stromal sarcoma.

  9. Soft tissue twisting injuries of the knee

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    Magee, T.; Shapiro, M. [Neuroimaging Inst., Melbourne, FL (United States)

    2001-08-01

    Twisting injuries occur as a result of differential motion of different tissue types in injuries with some rotational force. These injuries are well described in brain injuries but, to our knowledge, have not been described in the musculoskeletal literature. We correlated the clinical examination and MR findings of 20 patients with twisting injuries of the soft tissues around the knee. Design and patients: We prospectively followed the clinical courses of 20 patients with knee injuries who had clinical histories and MR findings to suggest twisting injuries of the subcutaneous tissues. Patients with associated internal derangement of the knee (i.e., meniscal tears, ligamentous or bone injuries) were excluded from this study. MR findings to suggest twisting injuries included linear areas of abnormal dark signal on T1-weighted sequences and abnormal bright signal on T2-weighted or short tau inversion recovery (STIR) sequences and/or signal to suggest hemorrhage within the subcutaneous tissues. These MR criteria were adapted from those established for indirect musculotendinous junction injuries. Results: All 20 patients presented with considerable pain that suggested internal derangement on physical examination by the referring orthopedic surgeons. All presented with injuries associated with rotational force. The patients were placed on a course of protected weight-bearing of the affected extremity for 4 weeks. All patients had pain relief by clinical examination after this period of protected weight-bearing. Twisting injuries of the soft tissues can result in considerable pain that can be confused with internal derangement of the knee on physical examination. Soft tissue twisting injuries need to be recognized on MR examinations as they may be the cause of the patient's pain despite no MR evidence of internal derangement of the knee. The demonstration of soft tissue twisting injuries in a patient with severe knee pain but no documented internal derangement on MR

  10. UTERINE ADENOMYOSIS AND AN ENDOMETRIAL POLYP IN A ROCK HYRAX (PROCAVIA CAPENSIS).

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    Holman, Holly J; Gailbreath, Katherine

    2016-12-01

    An 8-yr-old nulliparous, female rock hyrax ( Procavia capensis ) had intermittent mucoid to bloody discharge late winter-early spring for two consecutive years. Fragments of necrotic tissue, suspected to be vaginal or rectal in origin, were passed on two occasions. Physical examination, radiographs, and ultrasound did not identify the source of the tissue. Exploratory laparotomy and ovariohysterectomy were performed. On histopathology, a diagnosis of adenomyosis with a uterine polyp was made. Postsurgical complications included incisional infection and renal compromise that were medically managed and resolved. No further vaginal discharge was observed. This is the first report of uterine adenomyosis and endometrial polyp in a rock hyrax.

  11. Spectroscopic Monitoring of Kidney Tissue Ischemic Injury

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    Demos, S G; Fitzgerald, J T; Michalopoulou, A P; Troppmann, C

    2004-03-11

    Noninvasive evaluation of tissue viability of donor kidneys used for transplantation is an issue that current technology is not able to address. In this work, we explore optical spectroscopy for its potential to assess the degree of ischemic damage in kidney tissue. We hypothesized that ischemic damage to kidney tissue will give rise to changes in its optical properties which in turn may be used to asses the degree of tissue injury. The experimental results demonstrate that the autofluorescence intensity of the injured kidney is decreasing as a function of time exposed to ischemic injury. Changes were also observed in the NIR light scattering intensities most probably arising from changes due to injury and death of the tissue.

  12. Oxygen radicals, inflammation, and tissue injury.

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    Ward, P A; Warren, J S; Johnson, K J

    1988-01-01

    Inflammatory reactions often result in the activation and recruitment of phagocytic cells (e.g., neutrophils and/or tissue macrophages) whose products result in injury to the tissue. In killing of endothelial cells by activated neutrophils as well as in lung injury produced by either activated neutrophils or activated macrophages there is evidence that H2O2 and iron play a role. HO. may be a key oxygen product related to the process of injury. Endothelial cells in some vascular compartments may be susceptible to neutrophil mediated injury in a manner that is independent of oxygen radicals. On the basis of in vitro observations, a synergy exits between platelets and neutrophils, resulting in enhanced oxygen radical formation by the latter. Finally, the cytokines, interleukin 1 and tumor necrosis factor, released from macrophages have both direct stimulatory effects on oxygen radical formation in neutrophils and can "prime" macrophages for enhanced oxygen radical responses to other agonists. Cytokines may also alter endothelial cells rendering them more susceptible to oxygen radical mediated injury by neutrophils. This suggests a complex network of interactions between phagocytic cells and peptide mediators, the result of which is acute, oxygen radical mediated tissue injury.

  13. AT2 Receptor and Tissue Injury

    DEFF Research Database (Denmark)

    Namsolleck, Pawel; Recarti, Chiara; Foulquier, Sébastien;

    2014-01-01

    The renin-angiotensin system (RAS) plays an important role in the initiation and progression of tissue injuries in the cardiovascular and nervous systems. The detrimental actions of the AT1 receptor (AT1R) in hypertension and vascular injury, myocardial infarction and brain ischemia are well...... established. In the past twenty years, protective actions of the RAS, not only in the cardiovascular, but also in the nervous system, have been demonstrated. The so-called protective arm of the RAS includes AT2-receptors and Mas receptors (AT2R and MasR) and is characterized by effects different from...

  14. Significance of prevertebral soft tissue measurement in cervical spine injuries

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    Dai Liyang E-mail: lydai@etang.com

    2004-07-01

    Objective: The objective of this study was to evaluate the diagnostic value of prevertebral soft tissue swelling in cervical spine injuries. Materials and methods: A group of 107 consecutive patients with suspected injuries of the cervical vertebrae were reviewed retrospectively to identify the presence of prevertebral soft tissue swelling and to investigate the association of prevertebral soft tissue swelling with the types and degrees of cervical spine injuries. Results: Prevertebral soft tissue swelling occurred in 47 (43.9%) patients. Of the 47 patients, 38 were found with bony injury and nine were without. The statistic difference was significant (P<0.05). No correlation was demonstrated between soft tissue swelling and either the injured level of the cervical vertebrae or the degree of the spinal cord injury (P>0.05). Anterior element injuries in the cervical vertebrae had widening of the prevertebral soft tissue more than posterior element injuries (P<0.05). Conclusion: The diagnostic value of prevertebral soft tissue swelling for cervical spine injuries is significant, but the absence of this sign does not mean that further image evaluation can be spared.

  15. Physical Mechanisms of Soft Tissue Injury from Penetrating Ballistic Impact

    Science.gov (United States)

    2012-11-30

    2008; 64(6):1420-1426. 18. Gryth D, Rocksen D, Persson JK, Arborelius UP, Drobin D, Bursell J, Olsson LG, Kjellstrom BT. Severe lung contusion and...elastic tissues such as lungs and muscle, where the tissue tends to spring back into place with little damage from temporary stretch, most tissue...in a study comparing penetrating thoracic wounds caused by stab injuries to those caused by gunshot injuries, the occurrence of lung

  16. Properdin in complement activation and tissue injury.

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    Lesher, Allison M; Nilsson, Bo; Song, Wen-Chao

    2013-12-15

    The plasma protein properdin is the only known positive regulator of complement activation. Although regarded as an initiator of the alternative pathway of complement activation at the time of its discovery more than a half century ago, the role and mechanism of action of properdin in the complement cascade has undergone significant conceptual evolution since then. Despite the long history of research on properdin, however, new insight and unexpected findings on the role of properdin in complement activation, pathogen infection and host tissue injury are still being revealed by ongoing investigations. In this article, we provide a brief review on recent studies that shed new light on properdin biology, focusing on the following three topics: (1) its role as a pattern recognition molecule to direct and trigger complement activation, (2) its context-dependent requirement in complement activation on foreign and host cell surfaces, and (3) its involvement in alternative pathway complement-mediated immune disorders and considerations of properdin as a potential therapeutic target in human diseases.

  17. Cellular and Tissue Injury During Nonfreezing Cold Injury and Frostbite

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    1993-09-30

    Injuries - Rewarming Damages. 3 Biological, Angiological and Clinical Aspects. In "Disaster Medicine, Volume 3" (R. Frey and P. Safar, Ed.). Springer- Verlag...Kulka, J.P. Vasomotor microcirculatory insufficiency: observation of nonfreezing cold injury of the mouse ear. Angiology 12, 491-506 (1961). 38

  18. How to use PRICE treatment for soft tissue injuries.

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    Norton, Cormac

    2016-08-24

    Rationale and key points This article assists nurses to use the acronym PRICE (protection, rest, ice, compression and elevation) to guide the treatment of patients with uncomplicated soft tissue injuries to their upper or lower limbs. » Treatment of soft tissue injuries to limbs is important to reduce complications following injury, alleviate pain and ensure normal limb function is restored promptly. » Nurses should have an understanding of the rationale and evidence base supporting PRICE treatment of soft tissue injuries. » Providing accurate information to patients and carers about the management of soft tissue injuries and anticipated recovery time is an important aspect of treatment. » Further research is required to develop best practice in the treatment of soft tissue injuries. Reflective activity 'How to' articles can help you update your practice and ensure it remains evidence based. Apply this article to your practice. Reflect on and write a short account of: 1. How this article might change your practice when managing patients with soft tissue injuries to upper or lower limbs. 2. Positive elements of your current practice and those that could be enhanced. Subscribers can upload their reflective accounts at: rcni.com/portfolio.

  19. Soft Tissue Injuries in Hungarian and Austrian Clinical Diagnostic Reports

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    Fogarasi-Nuber Katalin

    2013-04-01

    Full Text Available Introduction: In addition to providing first aid, primary treating doctors are required to describe and register injuries acquired in accidents and assaults. They should do this with the highest possible accuracy, as this official document is often the only documentary evidence of soft tissue injuries in case a lawsuit is filed later. Characteristics of injuries may disappear faster with the healing process of the soft tissue, making it impossible for forensic experts to deduce the weapon involved. Consequently, terminological accuracy is a prerequisite for the appropriate reconstruction of the type and severity of injuries. This study aims at analysing reports on soft tissue injuries in Hungary and Austria from the terminological point of view. It is meant to reveal inaccuracies in the use of noun phrases impairing objective and accurate forensic assessment.

  20. Laser-induced interstitial thermotherapy (LITT) with the KTP 532 laser for the treatment of uterine adenomyosis

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    Chapman, Roxana; Chapman, Kenneth

    1997-05-01

    Adenomyosis is a condition in which the myometrium is infiltrated by endometrial glands and stroma. This results in myometrial hyperplasia, uterine enlargement and causes menorrhagia, dysmenorrhoea and dyspareunia for which there is no known cure other than hysterectomy. The success of LITT in the treatment of uterine leiomyomata suggested that this might also be effective for the treatment of adenomyosis. Initially LITT was carried out on patients with adenomyosis prior to hysterectomy, then on patients who had completed child-bearing and finally on those who desired a family. Not only were symptoms relieved but pregnancies occurred spontaneously. The KTP 532 nm component of the KTP/YAG laser, which is absorbed by red pigment, was used with a 600 micrometer fiber with a bare tip via a needle microstat at laparoscopy. Holes were drilled in the abnormal tissue 3 cm apart and the laser fiber then slowly withdrawn, the object being to coagulate the surrounding blood vessels and adenomyotic tissue. The number of joules required depended on the volume of tissue treated.

  1. Burn Injury: A Challenge for Tissue Engineers

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    Yerneni LK

    2009-01-01

    Full Text Available Ever since man invented fire he has been more frequently burning himself by this creation than by the naturally occurring bushfires. It is estimated that over 1.152 million people in India suffer from burn injuries requiring treatment every year and majority of them are women aged between 16-40 years and most of them occur in the kitchen. The treatment for burns basically involves autologous skin grafting, which originated in India more than two thousand years ago (Sushruta Samhita, is still the gold standard for the wound resurfacing, although, autografting is difficult where graftable donor sites are limited. Although, Cadaver skin, porcine or bovine xenografts are used alternatively over the past thirty years, modern approaches like the Bioengineering of skin substitutes emerged during the past 20 years as advanced wound management technologies with no social impediment. They can be broadly categorized as Acellular and Cellular biotechnological products. The acellular products like Alloderm (LifeCell Corporation, Integra (Integra Life Sciences act like template and depend on natural regeneration, while the cellular ones are either ‘Off-the-Shelf’ products like Apligraf (Organogenesis Inc and Orcel (Ortec International have allogenic elements and ‘home grown’ autologous cell products like Cultured Epithelial Autograft (CEA and epidermal-dermal composite skin use synthetic or natural non-human matrices. The CEA is based on the ex-vivo epidermal stem cell-expansion and our laboratory has been engaged in CEA technique development with innovative cost-effective approach and yielded promising preliminary clinical success. The basic methodological approach in CEA technique which is still clinically adopted by several developed countries involves the use of growth arrested mouse dermal fibroblasts as growth supportive matrix and is thus considered a drawback as a whole. Additionally, there is no superior enough method available to augment the

  2. Management of facial soft tissue injuries in children.

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    Vasconez, Henry C; Buseman, Jason L; Cunningham, Larry L

    2011-07-01

    Pediatric facial trauma can present a challenge to even the more experienced plastic surgeon. Injuries to the head and neck may involve bone and soft tissues with an assortment of specialized organs and tissue elements involved. Because of the active nature of children, facial soft tissue injuries can be diverse and extensive as well as some of the more common injuries a plastic surgeon is asked to treat. In 2007, approximately 800,000 patients younger than 15 years presented to emergency departments around the country with significant open wounds of the head that required treatment.In this review, we present the different types and regions of pediatric soft tissue facial trauma, as well as treatment options and goals of plastic surgery wound management. Special aspects, such as bite wounds, burns, pediatric analgesia, and antibiotic therapy, are also discussed.

  3. TREATMENT OF SOFT TISSUE INJURY BY PUNCTURING CLEFT-POINTS

    Institute of Scientific and Technical Information of China (English)

    Xu Yunxiang; Chen Guizhen

    2001-01-01

    Objective: To observe the therapeutic effect of acupuncture of "Xi" (Cleft)-points in treatment of soft tissue injury. Methods: 335 cases of soft tissue injury patients were divided into Cleft-point group (264 cases) and Ashipoint group (control group, 71 cases) randomly. In Cleft-point group, the 16 Cleft-points were used in combination with Ahshi points. In control group, only local Ahshi-points were punctured. The treatment was conducted once every day, with 5 sessions being a therapeutic course. After 2 courses of treatment, the therapeutic effect was analyzed.Results: Results showed that the therapeutic effect of cleft-point group was significantly better than that of control group (P<0.05), particularly in treatment of acute soft tissue. Conclusion: Cleft-point acupuncture has a better therapeutic effect in treatment of soft tissue injury in comparison with Ashi-point.

  4. Complement and Immunoregulation in Tissue Injury

    Science.gov (United States)

    2015-12-01

    rheumatoid arthritis . N Engl J Med 350(25): 2546-2548. 26. Hill, D. A., D. Artis. (2010). Intestinal bacteria and the regulation of immune cell...the rat intestinal epithelium. Gut 42(4): 530-537. 9. Carden, D. L. and D. N. Granger (2000). Pathophysiology of ischaemia-reperfusion injury. J Pathol

  5. MRI diagnosis of adenomyosis%子宫腺肌病的MRI诊断

    Institute of Scientific and Technical Information of China (English)

    杜龙庭; 陈辉; 陈小伟; 朱襄明; 李军

    2011-01-01

    目的:评价MRI对子宫腺肌病的诊断价值.方法:回顾性分析28例经手术病理证实的子宫腺肌病的MRI表现.结果:术前MRI均明确诊断.所有病例均表现子宫体不同程度增大,以后壁增大为明显.弥漫型子宫腺肌病10例:T1WI呈等信号,其中2例病变内可见点状高信号;T2WI呈稍低信号,其中6例病变内见散在的点状或小片状高信号,矢状面T2WI示子宫结合带弥漫性增厚,平均17mm.局限型子宫腺肌病18例:表现为肌层内单发或多发不规则形或圆形肿块状异常信号,直径2.1~8.5cm,平均3.7cm,病灶均与周围肌组织分界不清,T1WI呈等信号,其中9个病灶内可见点状高信号;T2WI呈稍低信号,其中13个病灶内见散在点、小片状高信号.20例行DWI检查,其中18例显示散在的多发点状或斑片状高信号,较T2WI显示清晰且高信号灶较T2WI为多.结论:MRI是诊断子宫腺肌病的无创性检查方法,子宫腺肌病的MRI表现具有特征性,合理选择扫描序列,有助于该病的术前诊断.%Objective:To investigate the value of MRI in the diagnosis of adenomyosis. Methods: MRI findings of 28 cases with surgically and histologically proved adcnomyosis were retrospectively analyzed. Results: Definite diagnosis was made in 28 cases by preoperative MR. All findings of these cases showed enlargement of the uterine body in different degrees, more obviously in the posterior walls. 10 cases were diffuse adenomyosis : they appeared as iso-intensity on T1 WI, 2 of them with punctate hyperintensity. On T2 WI these 10 cases showed slight hypointensity,6 of 10 cases showed punctate or patchy hyperintensity. On sagittal view of T2 WI the uterine junction zone was thickened with a mean thickness of 17mm. 18 cases were localized adenomyosis : irregular or round shape lesions were shown in the muscular layer,diameter between 2.1 and 8.5cm with a mean of 3.7cm,and boundaries of the lesions and the surrounded muscular tissues were

  6. Optical spectroscopy for the detection of ischemic tissue injury

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    Demos, Stavros; Fitzgerald, Jason; Troppmann, Christoph; Michalopoulou, Andromachi

    2009-09-08

    An optical method and apparatus is utilized to quantify ischemic tissue and/or organ injury. Such a method and apparatus is non-invasive, non-traumatic, portable, and can make measurements in a matter of seconds. Moreover, such a method and apparatus can be realized through optical fiber probes, making it possible to take measurements of target organs deep within a patient's body. Such a technology provides a means of detecting and quantifying tissue injury in its early stages, before it is clinically apparent and before irreversible damage has occurred.

  7. Injury Response of Resected Human Brain Tissue In Vitro.

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    Verwer, Ronald W H; Sluiter, Arja A; Balesar, Rawien A; Baaijen, Johannes C; de Witt Hamer, Philip C; Speijer, Dave; Li, Yichen; Swaab, Dick F

    2015-07-01

    Brain injury affects a significant number of people each year. Organotypic cultures from resected normal neocortical tissue provide unique opportunities to study the cellular and neuropathological consequences of severe injury of adult human brain tissue in vitro. The in vitro injuries caused by resection (interruption of the circulation) and aggravated by the preparation of slices (severed neuronal and glial processes and blood vessels) reflect the reaction of human brain tissue to severe injury. We investigated this process using immunocytochemical markers, reverse transcriptase quantitative polymerase chain reaction and Western blot analysis. Essential features were rapid shrinkage of neurons, loss of neuronal marker expression and proliferation of reactive cells that expressed Nestin and Vimentin. Also, microglia generally responded strongly, whereas the response of glial fibrillary acidic protein-positive astrocytes appeared to be more variable. Importantly, some reactive cells also expressed both microglia and astrocytic markers, thus confounding their origin. Comparison with post-mortem human brain tissue obtained at rapid autopsies suggested that the reactive process is not a consequence of epilepsy.

  8. OCT-based in vivo tissue injury mapping

    Science.gov (United States)

    Baran, Utku; Li, Yuandong; Wang, Ruikang K.

    2016-03-01

    Tissue injury mapping (TIM) is developed by using a non-invasive in vivo optical coherence tomography to generate optical attenuation coefficient and microvascular map of the injured tissue. Using TIM, the infarct region development in mouse cerebral cortex during stroke is visualized. Moreover, we demonstrate the in vivo human facial skin structure and microvasculature during an acne lesion development. The results indicate that TIM may help in the study and the treatment of various diseases by providing high resolution images of tissue structural and microvascular changes.

  9. The Field of Tissue Injury in the Lung and Airway

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    Steiling, Katrina; Ryan, John; Brody, Jerome S.; Spira, Avrum

    2009-01-01

    The concept of field cancerization was first introduced over six decades ago in the setting of oral cancer. Later, field cancerization involving histologic and molecular changes of neoplasms and adjacent tissue began to be characterized in smokers with or without lung cancer. Investigators also described a diffuse, non-neoplastic field of molecular injury throughout the respiratory tract that is attributable to cigarette smoking and susceptibility to smoking-induced lung disease. The potential molecular origins of field cancerization and the field of injury following cigarette smoke exposure in lung and airway epithelia are critical to understanding the impact of the field of injury on clinical diagnostics and therapeutics for smoking-induced lung disease. PMID:19138985

  10. National Training Course. Emergency Medical Technician. Paramedic. Instructor's Lesson Plans. Module VIII. Soft Tissue Injuries.

    Science.gov (United States)

    National Highway Traffic Safety Administration (DOT), Washington, DC.

    This instructor's lesson plan guide on soft tissue injuries is one of fifteen modules designed for use in the training of emergency medical technicians (paramedics). Six units of study are presented: (1) anatomy and physiology of the skin; (2) patient assessment for soft-tissue injuries; (3) pathophysiology and management of soft tissue injuries;…

  11. The Field of Tissue Injury in the Lung and Airway

    OpenAIRE

    Steiling, Katrina; Ryan, John; Brody, Jerome S.; Spira, Avrum

    2008-01-01

    The concept of field cancerization was first introduced over six decades ago in the setting of oral cancer. Later, field cancerization involving histologic and molecular changes of neoplasms and adjacent tissue began to be characterized in smokers with or without lung cancer. Investigators also described a diffuse, non-neoplastic field of molecular injury throughout the respiratory tract that is attributable to cigarette smoking and susceptibility to smoking-induced lung disease. The potentia...

  12. Development of Experimental Tissue Models for Blast Injury

    Science.gov (United States)

    Butler, Benjamin; Bo, Chiara; Williams, Alun; Jardine, Andy; Brown, Katherine

    2013-06-01

    There is a pressing need to better understand the relationship between the intensity of a blast wave and the clinical consequences for victims of an explosion. In order to quantitatively study how these factors correlate with one another, blast injury tissue models are being developed. Sections of larynx, trachea and pulmonary tissue were excised from a recently sacrificed pig and maintained on ice prior to testing. The samples were subjected to strain rates of between 0.001 s-1 and 1000 s-1 in the laboratory by using a Split Hopkinson Pressure Bar and quasi-static testing apparatus. During high strain rate testing, samples were housed in a polycarbonate chamber which permitted experimentation on tissue held in fluid. Data were analysed using 1, 2 and 3 wave analysis software in Matlab to yield information about the material properties of both undamaged and damaged tissues. In addition, macroscopic changes in tissue organization were also visualized using histopathological techniques. This work is being extended to cellular and animal models to derive more detailed information about the underlying molecular changes relating to blast-induced damage and repair. The Royal British Legion Centre for Blast Injury Studies.

  13. Undifferentiated connective tissue diseases-related hepatic injury

    Institute of Scientific and Technical Information of China (English)

    Ying Zhang; Fu-Kui Zhang; Xiao-Ning Wu; Tai-Ling Wang; Ji-Dong Jia; Bao-En Wang

    2008-01-01

    Hepatic injury is rarely associated with undifferentiated connective tissue diseases (UCTD).We report,here,a case of a middle-aged woman with UCTD-related hepatic injury,including its case history,clinical manifestations,laboratory findings,treatment and its short-term effect.The patient was admitted to the hospital with symptoms of fatigue,anorexia,low-grade fever and skin rashes.She had a past history of left knee joint replacement.Laboratory tests showed elevated levels of serum transaminase,IgG and globulin,accelerated erythrocyte sedimentation rate,eosinophilia and a high titer of antinuclear antibodies (1:320).Imaging studies showed interstitial pneumonitis and hydropericardium.Liver biopsy showed the features which were consistent with those of connective tissue diseases-related polyangitis.After treatment with a low-dose of oral prednisone,both symptoms and laboratory findings were significantly improved.UCTD-related hepatic injury should be considered in the differential diagnosis of connective tissue diseases with abnormal liver function tests.Lowdose prednisone may effectively improve both symptoms and laboratory tests.

  14. Aberrant innate immune activation following tissue injury impairs pancreatic regeneration.

    Directory of Open Access Journals (Sweden)

    Alexandra E Folias

    Full Text Available Normal tissue architecture is disrupted following injury, as resident tissue cells become damaged and immune cells are recruited to the site of injury. While injury and inflammation are critical to tissue remodeling, the inability to resolve this response can lead to the destructive complications of chronic inflammation. In the pancreas, acinar cells of the exocrine compartment respond to injury by transiently adopting characteristics of progenitor cells present during embryonic development. This process of de-differentiation creates a window where a mature and stable cell gains flexibility and is potentially permissive to changes in cellular fate. How de-differentiation can turn an acinar cell into another cell type (such as a pancreatic β-cell, or a cell with cancerous potential (as in cases of deregulated Kras activity is of interest to both the regenerative medicine and cancer communities. While it is known that inflammation and acinar de-differentiation increase following pancreatic injury, it remains unclear which immune cells are involved in this process. We used a combination of genetically modified mice, immunological blockade and cellular characterization to identify the immune cells that impact pancreatic regeneration in an in vivo model of pancreatitis. We identified the innate inflammatory response of macrophages and neutrophils as regulators of pancreatic regeneration. Under normal conditions, mild innate inflammation prompts a transient de-differentiation of acinar cells that readily dissipates to allow normal regeneration. However, non-resolving inflammation developed when elevated pancreatic levels of neutrophils producing interferon-γ increased iNOS levels and the pro-inflammatory response of macrophages. Pancreatic injury improved following in vivo macrophage depletion, iNOS inhibition as well as suppression of iNOS levels in macrophages via interferon-γ blockade, supporting the impairment in regeneration and the

  15. Facilitated assessment of tissue loss following traumatic brain injury

    Directory of Open Access Journals (Sweden)

    Anders eHånell

    2012-03-01

    Full Text Available All experimental models of traumatic brain injury (TBI result in a progressive loss of brain tissue. The extent of tissue loss reflects the injury severity and can be measured to evaluate the potential neuroprotective effect of experimental treatments. Quantitation of tissue volumes is commonly performed using evenly spaced brain sections stained using routine histochemical methods and digitally captured. The brain tissue areas are then measured and the corresponding volumes are calculated using the distance between the sections. Measurements of areas are usually performed using a general purpose image analysis software and the results are then transferred to another program for volume calculations. To facilitate the measurement of brain tissue loss we developed novel algorithms which automatically separate the areas of brain tissue from the surrounding image background and identify the ventricles. We implemented these new algorithms by creating a new computer program (SectionToVolume which also has functions for image organization, image adjustments and volume calculations. We analyzed brain sections from mice subjected to severe focal TBI using both SectionToVolume and ImageJ, a commonly used image analysis program. The volume measurements made by the two programs were highly correlated and analysis using SectionToVolume required considerably less time. The inter-rater reliability was high. Given the extensive use of brain tissue loss measurements in TBI research, SectionToVolume will likely be a useful tool for TBI research. We therefore provide both the source code and the program as attachments to this article.

  16. The thresholds and mechanisms of tissue injury by focused ultrasound

    Science.gov (United States)

    Simon, Julianna

    Therapeutic ultrasound is used in clinics around the world to treat ailments such as uterine fibroids, kidney stones, and plantar fasciitis. While many of the therapeutic effects of ultrasound are elicited by hyperthermia, bubbles can also interact with tissue to produce beneficial effects. For example, bubbles are used in boiling histotripsy to de-bulk tissue and are used in shock wave lithotripsy to break kidney stones. However, the same bubbles that break the kidney stones also damage the kidney, which is why bubble damage is a concern in every ultrasound application including fetal imaging. Whether the aim is to emulsify a tumor or image a fetus, understanding the thresholds and mechanisms of tissue injury by bubbles in an ultrasound field is important for all ultrasound applications and was the goal of this dissertation. One specific application of therapeutic ultrasound, known as boiling histotripsy, uses shock wave heating to explosively expand a millimeter-size boiling bubble at the transducer focus and fractionate bulk tissue. Yet it was unclear how the millimeter-size boiling or vapor bubble broke down the tissue into its submicron components. In this dissertation, we experimentally tested the hypothesis that ultrasonic atomization, or the emission of fine droplets from an acoustically excited liquid film, is the mechanism by which the millimeter-size boiling bubble in boiling histotripsy fractionates tissue into its submicron components. Using high speed photography, we showed that tissue can behave as a liquid such that a miniature acoustic fountain forms and atomization occurs within a millimeter-size cavity that approximates the boiling or vapor bubble produced by boiling histotripsy. The end result of tissue atomization was a hole in the tissue surface. After showing that tissue can be eroded by atomization, a series of experiments were conducted to determine the tissue properties that influence atomization. The results indicated that highly

  17. Endoplasmic reticulum stress, diabetes mellitus, and tissue injury.

    Science.gov (United States)

    Huang, Liu; Xie, Hong; Liu, Hao

    2014-01-01

    Endoplasmic reticulum (ER) stress is characterized by the accumulation of unfolded and misfolded proteins in the ER lumen. Unfolded and misfolded protein accumulation interferes with the ER function and triggers ER stress response. Thus, ER stress response, also called unfolded protein response (UPR), is an adaptive process that controls the protein amount in the ER lumen and the downstream protein demand. In normal conditions, the role of ER stress is to maintain ER homeostasis, restore ER function, and protect stressed cells from apoptosis, by coordinating gene expression, protein synthesis, and accelerating protein degradation through several molecular pathways. However, prolonged ER stress response plays a paradoxical role, which leads to cell damage, apoptosis, and concomitant tissue injuries. A number of tissue alterations are involved with diabetes mellitus progress and its comorbidities via ER stress. However, certain pharmacological agents affecting ER stress have been identified. In this review, we summarized the relationship between ER stress and insulin resistance development. Moreover, we aim to explain how ER stress influences type 2 diabetes mellitus (T2DM) development. In addition, we reviewed the literature on ER stress and UPR in three kinds of tissue injuries induced by T2DM. Finally, a retrospective analysis of the effects of anti-diabetes medications on ER stress is presented.

  18. Tissue plasminogen activator attenuates ventilatorinduced lung injury in rats

    Institute of Scientific and Technical Information of China (English)

    Liang-ti HUANG; Hsiu-chu CHOU; Leng-fang WANG; Chung-ming CHEN

    2012-01-01

    Aim:To test the hypothesis that the tissue plasminogen activator (tPA) may counteract the inhibitory effect ot plasminogen activator inhibitors (PAI) and attenuate lung injury in a rat model of ventilator-induced lung injury (VILI).Methods:Adult male Sprague-Dawley rats were ventilated with a HVZP (high-volume zero PEEP) protocol for 2 h at a tidal volume of 30 ml/kg,a respiratory rate of 25 breaths/min,and an inspired oxygen fraction of 21%.The rats were divided into 3 groups (n=7 for each):HVZP+tPA group receiving tPA (1.25 mg/kg,iv) 15 min before ventilation,HVZP group receiving HVZP+vehicle injection,and a control group receiving no ventilation.After 2 h of ventilation,the rats were killed; blood and lungs were collected for biochemical and histological analyses.Results:HVZP ventilation significantly increased total protein content and the concentration of macrophage inflammatory protein-2 (MIP-2) in the bronchoalveolar lavage fluid (BALF) as well as the lung injury score.Rats that received HVZP ventilation had significantly higher lung PAI-1 mRNA expression,plasma PAI-1and plasma D-dimer levels than the control animals,tPA treatment significantly reduced the BALF total protein and the lung injury score as compared to the HVZP group,tPA treatment also significantly decreased the plasma D-dimer levels and the HVZP ventilation-induced lung vascular fibrin thrombi,tPA treatment showed no effect on MIP-2 level in BALF.Conclusion:These results demonstrate that VILI increases lung PAI-1 mRNA expression,plasma levels of PAI-1 and D-limers,lung injury score and vascular fibrin deposition,tPA can attenuate VILI by decreasing capillary-alveolar protein leakage as well as local and systemic coagulation as shown by decreased lung vascular fibrin deposition and plasma D-dimers.

  19. Investigation of elemental changes in brain tissues following excitotoxic injury

    Science.gov (United States)

    Siegele, Rainer; Howell, Nicholas R.; Callaghan, Paul D.; Pastuovic, Zeljko

    2013-07-01

    Recently the ANSTO heavy ion microprobe has been used for elemental mapping of thin brain tissue sections. The fact that a very small portion of the proton energy is used for X-ray excitation combined with small variations of the major element concentrations makes μ-PIXE imaging and GeoPIXE analysis a challenging task. Excitotoxic brain injury underlies the pathology of stroke and various neurodegenerative disorders. Large fluxes in Ca+2 cytosolic concentrations are a key feature of the initiation of this pathophysiological process. In order to understand if these modifications are associated with changes in the elemental composition, several brain sections have been mapped with μ-PIXE. Increases in Ca+2 cytosolic concentrations were indicative of the pathophysiological process continuing 1 week after an initiating neural insult. We were able to measure significant variations in K and Ca concentration distribution across investigated brain tissue. These variations correlate very well with physiological changes visible in the brain tissue. Moreover, the obtained μ-PIXE results clearly demonstrate that the elemental composition changes significantly correlate with brain drauma.

  20. Investigation of elemental changes in brain tissues following excitotoxic injury

    Energy Technology Data Exchange (ETDEWEB)

    Siegele, Rainer, E-mail: rns@ansto.gov.au [Institute for Environmental Research, ANSTO, Locked Bag 2001, Kirrawee DC, NSW 2232 (Australia); Howell, Nicholas R.; Callaghan, Paul D. [Life Sciences, ANSTO, Locked Bag 2001, Kirrawee DC, NSW 2232 (Australia); Pastuovic, Zeljko [Institute for Environmental Research, ANSTO, Locked Bag 2001, Kirrawee DC, NSW 2232 (Australia)

    2013-07-01

    Recently the ANSTO heavy ion microprobe has been used for elemental mapping of thin brain tissue sections. The fact that a very small portion of the proton energy is used for X-ray excitation combined with small variations of the major element concentrations makes μ-PIXE imaging and GeoPIXE analysis a challenging task. Excitotoxic brain injury underlies the pathology of stroke and various neurodegenerative disorders. Large fluxes in Ca{sup +2} cytosolic concentrations are a key feature of the initiation of this pathophysiological process. In order to understand if these modifications are associated with changes in the elemental composition, several brain sections have been mapped with μ-PIXE. Increases in Ca{sup +2} cytosolic concentrations were indicative of the pathophysiological process continuing 1 week after an initiating neural insult. We were able to measure significant variations in K and Ca concentration distribution across investigated brain tissue. These variations correlate very well with physiological changes visible in the brain tissue. Moreover, the obtained μ-PIXE results clearly demonstrate that the elemental composition changes significantly correlate with brain drauma.

  1. Variable uterine uptake of FDG in adenomyosis during concurrent chemoradiation therapy for cervical cancer

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Jeong Il; Huh, Seung Jae; Kim, Young Il; Kim, Tae Joong; Park, Byung Kwan [Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

    2011-09-15

    To avoid improper tumor volume contouring in radiation therapy (RT) and other invasive procedures, we report a case of uterine adenomyosis showing increased 18F-fl uorodeoxyglucose (FDG) uptake on positron emission tomography (PET)/computed tomography (CT) mimicking malignant tumor in a 44-year-old woman during concurrent chemoradiation therapy (CCRT) for uterine cervical cancer. The adenomyosis was not associated with her menstrual cycle or with normal endometrium uptake, and it resolved one month after completion of RT. This case indicates that uterine adenomyosis in a premenopausal woman may show false positive uptake of 18FDG-PET/CT associated with CCRT.

  2. Regeneration of the skin and muscle tissue in rainbow trout (Oncorhynchus mykiss) following mechanical injury

    DEFF Research Database (Denmark)

    Ingerslev, Hans-Christian; Nielsen, Michael Engelbrecht

    Mechanical injury induced by needles penetrating the skin and underlying muscle tissue in rainbow trout (Oncorhynchus mykiss) was used as a model to study the initial phase(s) of tissue regeneration. Tissue regeneration in humans is characterised by four phases; hemostatis, inflammation......, proliferation and remodelling. We investigated the expression of genes traditionally being important in these processes untill 7 days after the tissue damage in order to find inducible genetic markers following mechanical injury....

  3. Autophagy, Innate Immunity and Tissue Repair in Acute Kidney Injury

    Directory of Open Access Journals (Sweden)

    Pu Duann

    2016-05-01

    Full Text Available Kidney is a vital organ with high energy demands to actively maintain plasma hemodynamics, electrolytes and water homeostasis. Among the nephron segments, the renal tubular epithelium is endowed with high mitochondria density for their function in active transport. Acute kidney injury (AKI is an important clinical syndrome and a global public health issue with high mortality rate and socioeconomic burden due to lack of effective therapy. AKI results in acute cell death and necrosis of renal tubule epithelial cells accompanied with leakage of tubular fluid and inflammation. The inflammatory immune response triggered by the tubular cell death, mitochondrial damage, associative oxidative stress, and the release of many tissue damage factors have been identified as key elements driving the pathophysiology of AKI. Autophagy, the cellular mechanism that removes damaged organelles via lysosome-mediated degradation, had been proposed to be renoprotective. An in-depth understanding of the intricate interplay between autophagy and innate immune response, and their roles in AKI pathology could lead to novel therapies in AKI. This review addresses the current pathophysiology of AKI in aspects of mitochondrial dysfunction, innate immunity, and molecular mechanisms of autophagy. Recent advances in renal tissue regeneration and potential therapeutic interventions are also discussed.

  4. Focal adenomyosis (intramural endometriotic cyst) in a very young patient - differential diagnosis with uterine fibromatosis

    Science.gov (United States)

    Manta, L; Suciu, N; Constantin, A; Toader, O; Popa, F

    2016-01-01

    Introduction. Adenomyosis is a widespread disease usually affecting the late reproductive years of the women’s life, which has a great impact on their fertility. The most common form is diffuse adenomyosis, while focal adenomyosis, a cystic variant, is very rare, particularly in patients younger than 30 years old. Materials and methods. We reported a rare case of a 20-year-old Caucasian woman with cystic adenomyosis who was admitted in our service with severe chronic pelvic pain, dysmenorrhea, and menorrhagia, who had received conservative surgical treatment to preserve fertility and improve her obstetrical prognosis. Results and Discussions. Although the necrobiosis of a uterine fibroid was suspected preoperatively, the extemporaneous histopathological exam revealed adenomyosis associated with fibroleiomyoma with hyaline dystrophy and multiple foci of endometriosis of cystic formation in the wall of a young woman without any risk factors. Conclusion. Although a rare lesion in young patients, cystic adenomyosis should be considered when chronic pelvic pain is exacerbated during menstruation and is associated with a uterine tumor. In young patients, the differential diagnosis should be made with uterine malformations (hematometra), necrobiosis of uterine fibroids, pelvic endometriosis. The surgical treatment should be conservative with the excision of the lesion, always taking into account fertility preservation in young patients. PMID:27453751

  5. Laparoscopic resection versus myolysis in the management of symptomatic uterine adenomyosis: alternatives to conventional treatment

    Directory of Open Access Journals (Sweden)

    Wachyu Hadisaputra

    2006-03-01

    Full Text Available Effective therapy preserving reproductive function in adenomyosis is warranted. From June 2003 to June 2004, patients diagnosed as having adenomyosis by transvaginal ultrasound and had symptoms of menorrhagia, dysmenorrhea, and pelvic pain were randomly allocated to either receive laparoscopic resection or myolysis. GnRH analog was given for 3 cycles after surgery. Within 6 months, symptoms were evaluated using questionnaires and at the end of follow up, adenomyosis volume was assessed by transvaginal ultra-sound. There were 20 patients included, 10 patients had resection and the rest underwent myolysis. Both procedures did not yield sig-nificant complications. Subjective evaluation by questionnaires was done in all patients. Three patients could not be evaluated objec-tively by transvaginal ultrasound, 2 patients resigned and 1 was pregnant. There was no significant difference in menorrhagia and dysmenorrhea reduction score between the 2 groups (p=0.399 and 0.213, respectively. In both groups, dysmenorrhea was reduced significantly after treatment. No significant statistical difference was found in median adenomyosis volume increment (p=0.630 be-tween the resection (median=+15.35% (-100-159} and myolysis groups (median=+48.43% (-100-553. Five patients were pregnant, 3 from the resection group and 2 from the myolysis group. Uterine rupture was found in 1 patient (from the myolysis group at the age of 8 months of pregnancy. The effectiveness of laparoscopic adenomyosis resection was not significantly different compared with lapa-rascopic myolysis as an alternative conservative surgery in treating symptomatic adenomyosis. Myolysis was not recommended for women who wish to be pregnant. (Med J Indones 2006; 15:9-17Keywords: laparascopy, resection, myolysis, conservative surgery, symptomatic adenomyosis

  6. Long-term allergen exposure induces adipose tissue inflammation and circulatory system injury.

    Science.gov (United States)

    Jung, Chien-Cheng; Su, Huey-Jen

    2016-05-01

    The purpose of this study was to study whether allergen exposure can induce inflammation and lower the anti-inflammation levels in serum and in adipose tissues, and further develop cardiovascular injury. Our data showed that heart rate was significantly higher in the OVA-challenged mice compared to control mice. Moreover, there were higher expressions of pro-inflammation genes in the OVA-challenged mice in adipose tissues, and the expressions of anti-inflammation genes were lower. The levels of inflammation mediators were associated in serum and adipose tissues. The level of circulatory injury lactate dehydrogenase was significantly associated with the levels of E-selectin, resistin and adiponectin in the serum. The hematoxylin and eosin and immunohistochemistry stains indicated the OVA-challenged mice had higher levels of inflammation. In summary, the current study demonstrated allergen exposure can cause cardiovascular injury, and inflammatory mediators in adipose tissues play an important role in the pathogenesis of cardiovascular injury.

  7. Combination of thermographic and ultrasonographic assessments for early detection of deep tissue injury.

    Science.gov (United States)

    Higashino, Takuya; Nakagami, Gojiro; Kadono, Takafumi; Ogawa, Yuki; Iizaka, Shinji; Koyanagi, Hiroe; Sasaki, Sanae; Haga, Nobuhiko; Sanada, Hiromi

    2014-10-01

    Early detection and intervention of deep tissue injury are important to lead good outcome. Although the efficiency of ultrasonographic assessment of deep tissue injury has been reported previously, it requires a certain level of skill for accurate assessment. In this study, we present an investigation of the combination of thermographic and ultrasonographic assessments for early detection of deep tissue injury. We retrospectively reviewed 28 early-stage pressure ulcers (21 patients) presenting at the University of Tokyo Hospital between April 2009 and February 2010, surveying the associated thermographic and ultrasonographic findings. The wound temperature patterns were divided into low, even and high compared with the surrounding skin. Ultrasonographic findings were classified into unclear layer structure, hypoechoic lesion, discontinuous fascia and heterogeneous hypoechoic area. All 13 ulcers that were associated with low temperature showed good outcome; three ulcers had even temperatures and 12 ulcers showed high temperature on thermographic assessment. The two deep tissue injuries were rated high on thermographic assessment and showed heterogeneous hypoechoic area findings on ultrasonographic assessment. No non-deep tissue injury lesion was associated with these two findings simultaneously. The combination of thermographic and ultrasonographic assessments is expected to increase the accuracy of the early detection of deep tissue injuries.

  8. Kidney Injury Molecule-1 Protects against Gα12 Activation and Tissue Damage in Renal Ischemia-Reperfusion Injury

    Science.gov (United States)

    Ismail, Ola Z.; Zhang, Xizhong; Wei, Junjun; Haig, Aaron; Denker, Bradley M.; Suri, Rita S.; Sener, Alp; Gunaratnam, Lakshman

    2016-01-01

    Ischemic acute kidney injury is a serious untreatable condition. Activation of the G protein α12 (Gα12) subunit by reactive oxygen species is a major cause of tissue damage during renal ischemia-reperfusion injury. Kidney injury molecule-1 (KIM-1) is a transmembrane glycoprotein that is highly up-regulated during acute kidney injury, but the physiologic significance of this up-regulation is unclear. Here, we report for the first time that Kim-1 inhibits Gα12 activation and protects mice against renal ischemia-reperfusion injury. We reveal that Kim-1 physically interacts with and inhibits cellular Gα12 activation after inflammatory stimuli, including reactive oxygen species, by blocking GTP binding to Gα12. Compared with Kim-1+/+ mice, Kim-1−/− mice exhibited greater Gα12 and downstream Src activation both in primary tubular epithelial cells after in vitro stimulation with H2O2 and in whole kidneys after unilateral renal artery clamping. Finally, we show that Kim-1–deficient mice had more severe kidney dysfunction and tissue damage after bilateral renal artery clamping, compared with wild-type mice. Our results suggest that KIM-1 is an endogenous protective mechanism against renal ischemia-reperfusion injury through inhibition of Gα12. PMID:25759266

  9. Tissue tears in the white matter after lateral fluid percussion brain injury in the rat: relevance to human brain injury.

    Science.gov (United States)

    Graham, D I; Raghupathi, R; Saatman, K E; Meaney, D; McIntosh, T K

    2000-02-01

    A characteristic feature of severe diffuse axonal injury in man is radiological evidence of the "shearing injury triad" represented by lesions, sometimes haemorrhagic, in the corpus callosum, deep white matter and the rostral brain stem. With the exception of studies carried out on the non-human primate, such lesions have not been replicated to date in the multiple and diverse rodent laboratory models of traumatic brain injury. The present report describes tissue tears in the white matter, particularly in the fimbria of Sprague-Dawley rats killed 12, 24, and 48 h and 7 days after lateral fluid percussion brain injury of moderate severity (2.1-2.4 atm). The lesions were most easily seen at 24 h when they appeared as foci of tissue rarefaction in which there were a few polymorphonuclear leucocytes. At the margins of these lesions, large amounts of accumulated amyloid precursor protein (APP) were found in axonal swellings and bulbs. By 1 week post-injury, there was macrophage infiltration with marked astrocytosis and early scar formation. This lesion is considered to be due to severe deformation of white matter and this is the first time that it has been identified reproducibly in a rodent model of head injury under controlled conditions.

  10. A Retrospective Review of Iatrogenic Skin and Soft Tissue Injuries

    Directory of Open Access Journals (Sweden)

    Tae Geun Lee

    2012-07-01

    Full Text Available Background Even though the quality of medical and surgical care has improved remarkablyover time, iatrogenic injuries that require surgical treatment including injuries caused by castand elastic bandage pressure, extravasation, and dopamine-induced ischemia still frequentlyoccur. The goal of this study was to estimate the incidence and analyze the distribution ofiatrogenic injuries referred to our department.Methods A retrospective clinical review was performed from April 2006 to November 2010. Intotal, 196 patients (116 females and 80 males were referred to the plastic surgery departmentfor the treatment of iatrogenic injuries. We analyzed the types and anatomic locations ofiatrogenic complications, along with therapeutic results.Results An extravasation injury (65 cases, 37.4% was the most common iatrogeniccomplication in our study sample, followed by splint-induced skin ulceration, dopamineinducednecrosis, prefabricated pneumatic walking brace-related wounds and elasticbandage-induced wounds. Among these, prefabricated pneumatic walking brace-relatedcomplication incidence increased the most during the 5-year study period.Conclusions The awareness of the very common iatrogenic complications and its causes mayallow physicians to reduce their occurrence and allow for earlier detection and referral to aplastic surgeon. We believe this is the first study to analyze iatrogenic complications referredto a plastic surgery department in a hospital unit.

  11. Maxillofacial trauma with emphasis on soft-tissue injuries in Malaysia.

    Science.gov (United States)

    Hussaini, H M; Rahman, N A; Rahman, R A; Nor, G M; Ai Idrus, S M; Ramli, R

    2007-09-01

    Soft-tissue injuries with or without facial bone involvement are the most common presentation following maxillofacial trauma. The objective of this study was to look at the distribution, pattern and type of soft-tissue injury in relation to aetiology. Records of patients over a period of 5 years (1998-2002), who sustained maxillofacial injuries and were treated at Kajang Hospital, a secondary referral hospital, were reviewed. Out of 313 patients with maxillofacial injuries, 295 patients sustained soft-tissue injuries. Males (79%) between 21 and 30 years old (34%) were the majority of patients. Road-traffic accident was the main cause of soft-tissue injuries (75%) with motorcycle accident being the most frequent (40%). The upper lips (23%) and the lower lips (18%) were the most common extraoral site involved, while the labial mucosa and sulcular areas, both accounting for 21%, were the most common intraoral sites. Stringent road-traffic regulations should be practiced in developing countries, as morbidity arising from road-traffic accidents poses a national economic and social problem.

  12. Factors associated with deep tissue injury in male wheelchair basketball players of a Japanese national team

    Directory of Open Access Journals (Sweden)

    Hirotaka Mutsuzaki

    2014-04-01

    Full Text Available Maintenance of the sporting activity of elite athletes in adapted sports can be difficult if a secondary disorder, such as a pressure ulcer, occurs. Pressure ulcers result from deep tissue injuries by external pressure. The purpose of this study was to use ultrasonography to investigate deep tissue injuries in male wheelchair basketball players of a Japanese national team, and to determine factors associated with the injuries (e.g., body mass index, class of wheelchair basketball, underlying disease, length of athletic career, and whether use of wheelchair is primarily for playing basketball. Twenty male Japanese wheelchair basketball players on the national team for the 2012 London Paralympic Games (12 representative players and eight candidate representative players participated in this study. The sacral region and bilateral ischial regions in each athlete were examined by ultrasonography to detect low-echoic lesions indicative of deep tissue injuries. Nine (45% players had low-echoic lesions, which were detected in 10 of 60 areas. Eight lesions were detected in the sacral region and two lesions were detected in the ischial region. More players with spinal cord injury had low-echoic lesions [9 (69.2% of 13 players], compared to players with skeletal system disease [0 (0% of 7 players, p = 0.002]. Players who used a wheelchair in daily life were more likely to have low-echoic lesions [8 (66.74% of 12 players], compared to players who primarily used a wheelchair for playing basketball [1 (12.5% of 8 players, p = 0.010]. Deep tissue injuries were detected in 45% of male Japanese wheelchair basketball players on the national team. Players with spinal cord injury and players who used a wheelchair in daily life were more likely to have deep tissue injuries, particularly in the sacral region. The lesions were small, but a periodic medical check should be performed to maintain athletes' sporting life.

  13. [Combination of intersticial cystitis and adenomyosis in females suffering from chronic pelvic pain syndrome].

    Science.gov (United States)

    Neĭmark, A I; Shelkovnikova, N V

    2011-01-01

    General and endoscopic examinations of 25 patients aged 40-48 years with chronic pelvic pain syndrome (CPPS) revealed combination of two urogenital diseases: interstitial cystitis and adenomyosis of the second-third degree. The complex treatment including a course of lavomax immunocorrection relieved CPPS in 72% (18) patients, reduced the number of recurrences of chronic pelvic diseases in 16% (4) patients. The therapeutic complex proposed may serve an alternative to surgical treatment of adenomyosis of the second-third degree which is uncomplicated with posthemorrhagic iron-deficiency anemia, manifests with pain only and associated with interstitial cystitis.

  14. Risk factors for a pressure-related deep tissue injury: a theoretical model.

    Science.gov (United States)

    Gefen, Amit

    2007-06-01

    Pressure-related deep tissue injury is the term recommended by the United States National Pressure Ulcer Advisory Panel to describe a potentially life-threatening form of pressure ulcers, characterized by the presence of necrotic tissue under intact skin, and associated with prolonged compression of muscle tissue under bony prominences. In this study, a theoretical model was used to determine the relative contributions of the backrest inclination angle during prolonged wheelchair sitting, the muscle tissue stiffness and curvature of the ischial tuberosities (ITs) to the risk for injury in the gluteus muscles that pad the IT bones during sitting. The model is based on Hertz's theory for analysis of contact pressures between a rigid half-sphere (bone) and an elastic half-space (muscle). Hertz's theory is coupled with an injury threshold and damage law for muscle-both obtained in previous studies in rats. The simulation outputs the time-dependent bone-muscle contact pressures and the injured area in the gluteus. We calculated the full-size (asymptotic) injured area in the gluteus and the time for injury onset for different sitting angles alpha (90-150 degrees), muscle tissue long-term shear moduli G (250-1,200 Pa) and bone diameters D (8-18 mm). We then evaluated the sensitivity of model results to variations in these parameters, in order to determine how injury predictions are affected. In reclined sitting (alpha=150 degrees) the full-size injured area was approximately 2.1-fold smaller and the time for injury onset was approximately 1.3-fold longer compared with erect sitting (alpha=90 degrees). For greater G the full-size injured area was smaller but the time for injury onset was shorter, e.g., increasing G from 250 to 1200 Pa decreased the full-size injured area approximately 2.5-fold, but shortened the time for injury onset 6.2-fold. For smaller D the time for injury onset dropped, e.g., decreased approximately 1.5-fold when D decreased from 18 to 8 mm

  15. Burn-injury affects gut-associated lymphoid tissues derived CD4+ T cells☆

    OpenAIRE

    Fazal, Nadeem; Shelip, Alla; Alzahrani, Alhusain J.

    2013-01-01

    After scald burn-injury, the intestinal immune system responds to maintain immune balance. In this regard CD4+T cells in Gut-Associated Lymphoid Tissues (GALT), like mesenteric lymph nodes (MLN) and Peyer's patches (PP) respond to avoid immune suppression following major injury such as burn. Therefore, we hypothesized that the gut CD4+T cells become dysfunctional and turn the immune homeostasis towards depression of CD4+ T cell-mediated adaptive immune responses. In the current study we show ...

  16. Erythropoietin-mediated tissue protection: reducing collateral damage from the primary injury response.

    Science.gov (United States)

    Brines, M; Cerami, A

    2008-11-01

    In its classic hormonal role, erythropoietin (EPO) is produced by the kidney and regulates the number of erythrocytes within the circulation to provide adequate tissue oxygenation. EPO also mediates other effects directed towards optimizing oxygen delivery to tissues, e.g. modulating regional blood flow and reducing blood loss by promoting thrombosis within damaged vessels. Over the past 15 years, many unexpected nonhaematopoietic functions of EPO have been identified. In these more recently appreciated nonhormonal roles, locally-produced EPO signals through a different receptor isoform and is a major molecular component of the injury response, in which it counteracts the effects of pro-inflammatory cytokines. Acutely, EPO prevents programmed cell death and reduces the development of secondary, pro-inflammatory cytokine-induced injury. Within a longer time frame, EPO provides trophic support to enable regeneration and healing. As the region immediately surrounding damage is typically relatively deficient in endogenous EPO, administration of recombinant EPO can provide increased tissue protection. However, effective use of EPO as therapy for tissue injury requires higher doses than for haematopoiesis, potentially triggering serious adverse effects. The identification of a tissue-protective receptor isoform has facilitated the engineering of nonhaematopoietic, tissue-protective EPO derivatives, e.g. carbamyl EPO, that avoid these complications. Recently, regions within the EPO molecule mediating tissue protection have been identified and this has enabled the development of potent tissue-protective peptides, including some mimicking EPO's tertiary structure but unrelated in primary sequence.

  17. Expressions of oxytocin receptor and estrogen receptor in adenomyosis and their relationships with dysmenorrhea%OTR、ER在子宫腺肌病中的表达及与痛经的关系

    Institute of Scientific and Technical Information of China (English)

    徐影; 赵艳晖; 邹颖刚; 张炜旸

    2012-01-01

    目的:探讨OTR、ER在子宫腺肌病患者在位及病灶组织中的表达及其与痛经的关系.方法:采用免疫组化法检测30例子宫腺肌病在位和异位内膜中OTR、ER的表达,以子宫正常肌层组织和在位内膜为对照.结果:在子宫腺肌病灶组织中OTR、ER的表达高于在位内膜组及对照组(P<0.05);在子宫腺肌病组的在位内膜和病灶组织中,痛经组OTR、ER的表达高于无痛经组及对照组(P<0.05);在不同痛经程度分组中OTR、ER的表达依次降低.在子宫腺肌病的在位内膜和病灶组织中,OTR、ER两者之间存在正相关关系.结论:子宫腺肌病组织中OTR、ER的表达随痛经程度增强而增加,在子宫腺肌病痛经的发生与发展间可能存在协同作用.%Objective; To explore the expression levels of oxytocin receptor and estrogen receptor in eutopic endometrium and ec-topic endometrium of the patients with adenomyosis and their relationships with dysmenorrhea Methods; Immunohistochemical staining was used to detect the expression levels of oxytocin receptor and estrogen receptor in eutopic endometrium and ectopic endometrium of 30 patients with adenomyosis, normal uterine muscular tissue and eutopic endometrium were designed as control group. Results: The expression levels of oxytocin receptor and estrogen receptor in ectopic endometrium of adenomyosis group were significantly higher than those in eutopic endometrium of adenomyosis group and control group ( P < 0.05); in eutopic endometrium and ectopic endometrium of adenomyosis group, the expression levels of oxytocin receptor and estrogen receptor in dysmenorrhea subgroup were significantly higher than those in non - dysmenorrhe-a subgroup ( P < 0.05 ) ; the expression levels of oxytocin receptor and estrogen receptor decreased sequently in the subgroups with different degrees of dysmenorrhea; in eutopic endometrium and ectopic endometrium of adenomyosis group, there was a positive correlation

  18. Pattern, severity, and management of cranio-maxillofacial soft-tissue injuries in Port Harcourt, Nigeria

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    Akinbami Babatunde Olayemi

    2013-01-01

    Full Text Available Background: The pattern of craniofacial soft-tissue injuries occurring either in isolation or in association with fractures vary in different societies and is multiply influenced. The effects are enormous because of the prominence of the face; therefore, the purpose of this study was to document any changing pattern, severity and management of these craniofacial injuries in our center. Patients and Method: Cranio-maxillofacial region was classified into upper, middle and lower face. The cause, type, and site of the injuries were documented. Gunshot injuries were further categorized as penetrating, perforating or avulsions. Further, classification of injuries into mild, moderate, and severe was carried out based on multiple factors. Result: A total of 126 patients with soft-tissue injuries presented to our hospital out of which 85 (67.5% were males and 41 (32.5 were females. The age range of the patients was between 10 months and 90 years with a mean ± SD of 26.4 ± 15.5 years. Road traffic accident was the most common etiology of which vehicular accidents constituted 50 (54.9% and the motorcycle was 2 (2.2%. Assault contributed 16 (17.6% while cases due to gun shots were 13 (14.3%. A total of 19 (15.1% patients had associated head injuries, 11 (8.7% patients had craniofacial fractures involving any of the bones while 3 (2.4% patients had limb fractures and 2 (1.6% patients had rib fractures. There were 51 (41.8% cases classified as mild injuries, 37 (30.3% cases as moderate injuries and 24 (19.7% cases as severe injuries. Total of 126 cases managed, 121 (96.0% received primary closure of the wounds while 5 (4.0% received delayed closure under general anesthesia.

  19. Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis

    Institute of Scientific and Technical Information of China (English)

    Jing-Hua Shi; Li Jin; Jin-Hua Leng; Jing-He Lang

    2016-01-01

    Background:Adenomyosis (AM) has impaired contraction.This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells (MSMCs) of AM.Methods:Uterine tissue samples from 22 patients (cases) with histologically confirmed AM and 12 (controls) with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calcium-and voltage-sensitive K+ channel (BKCa)-α/β subunits,voltage-gated potassium channel (Kv) 4.2,and Kv4.3.Student's t-test was used to compare the expression.Results:The BKCa-α/β subunits,Kv4.2,and Kv4.3 were located in smooth muscle cells,glandular epithelium,and stromal cells.However,BKCa-β subunit expression in endometrial glands of the controls was weak,and Kv4.3 was almost undetectable in the controls.The expression of BKCa-α messenger RNA (mRNA) (0.62 ± 0.19-fold decrease,P < 0.05) and Kv4.3 mRNA (0.67 ± 0.20-fold decrease,P < 0.05) decreased significantly in the M SMCs of the control group compared with the AM group.However,there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA.Conclusions:The BKCa-α mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group,that might cause the abnormal uterus smooth muscle contractility,change the microcirculation of uterus to accumulate the inflammatory factors,impair the endometrium further,and aggravate the pain.

  20. Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis

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    Jing-Hua Shi

    2016-01-01

    Full Text Available Background: Adenomyosis (AM has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells (MSMCs of AM. Methods: Uterine tissue samples from 22 patients (cases with histologically confirmed AM and 12 (controls with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calcium- and voltage-sensitive K + channel (BKCa-α/β subunits, voltage-gated potassium channel (Kv 4.2, and Kv4.3. Student′s t-test was used to compare the expression. Results: The BKCa-α/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-α messenger RNA (mRNA (0.62 ± 0.19-fold decrease, P < 0.05 and Kv4.3 mRNA (0.67 ± 0.20-fold decrease, P < 0.05 decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA. Conclusions: The BKCa-α mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain.

  1. Cystic adenomyosis spreading into subserosal-peduncolated myoma: How to explain it?

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    Gloria Calagna

    2015-01-01

    Conclusions: In this case, the peculiar growth pattern of cystic adenomyosis in a myoma represents a singular condition rarely reported in the medical literature. We therefore support the pathogenetic theory that the disease might have been caused by direct proliferation of endometrial cells within a peduncolated- subserosal myoma.

  2. Therapeutic effectiveness of instrument-assisted soft tissue mobilization for soft tissue injury: mechanisms and practical application

    Science.gov (United States)

    Kim, Jooyoung; Sung, Dong Jun; Lee, Joohyung

    2017-01-01

    This article reviews the mechanism and effects of instrument-assisted soft tissue mobilization (IASTM), along with guidelines for its practical application. IASTM refers to a technique that uses instruments to remove scar tissues from injured soft tissues and facilitate healing process through formation of new extracellular matrix proteins such as collagen. Recently, frequent use of this instrument has increased in the fields of sports rehabilitation and athlete training. Some experimental studies and case reports have reported that IASTM can significantly improve soft tissue function and range of motion following sports injury, while also reducing pain. Based on the previous studies, it is thought that IASTM can help shorten the rehabilitation period and time to return to sports among athletes and ordinary people who have suffered sports injuries. However, few experimental studies of the mechanisms and effects of IASTM have examined, while case reports have accounted for the majority of articles. In the future, the scientific basis of IASTM and its reliability should be provided through well-designed experimental studies on humans. Moreover, IASTM studies that have mostly focused on tendons need to broaden their scope toward other soft tissues such as muscles and ligaments.

  3. Tissue Mechanics and Its Relationship to Athletic Injury Prevention.

    Science.gov (United States)

    Alexander, Marion J. L.

    Three types of dense fibrous connective tissue are described: articular cartilage; tendon; and ligament; and the characteristics of each are related to its function. Articular cartilage is the smooth covering over the surface of bone at joints, and is comprised of up to 80 percent water. This smooth covering serves to decrease the friction between…

  4. Effects of cefodizime on chemokines of liver tissues in mice with immunological hepatic injury

    Institute of Scientific and Technical Information of China (English)

    WANG Peng; KAN Quan-cheng; YU Zu-jiang; LI Ling; PAN Xue

    2011-01-01

    Background Chronic hepatic inflammation is characterized by the accumulation of lymphocytes as a consequence of increased recruitment from the blood and retention within the tissue at sites of infection. CXC chemokine ligand 16 (CXCL16) mRNA has been detected in both inflamed and normal liver tissues and is strongly upregulated in the injured liver tissues in a murine model. The aim of this study was to investigate the effect of cefodizime on CXCL16 mRNA of liver tissues in mice with immunological hepatic injury.Methods The murine model of immunological hepatic injury was induced by Bacillus Calmette Guerin and Lipoposaccharide. The mice with immunological hepatic injury were randomly assigned to the model group, the cefodizime group and the ceftriaxone group. The three groups were continuously given agents for seven days and CXCL16 mRNA of liver tissue was determined and contrasted with the control group treated by normal saline. Reverse transcription-polymerase chain reaction was used to assay CXCL16 mRNA levels in liver tissues.Results The expressions of CXCL16 mRNA were significantly higher in the model group and the ceftriaxone group than in the control group and the cefodizime group (P <0.05), indicating the mice in the model group and the ceftriaxone group were immunodeficient. There was no statistical difference in the expressions of CXCL16 mRNA between the control group and the cefodizime group. Similarly, no statistical difference in the expressions of CXCL16 mRNA between the model group and the ceftriaxone group was detected (P >0.05).Conclusion Cefodizime effectively reduces the infiltration of lymphocytes into liver tissues and alleviates the liver damage by decreasing CXCL16 mRNA in liver tissues in mice with immunological hepatic injury.

  5. FAT EMBOLISM SYNDROME WITHOUT OBJECTIVE EVIDENCE OF BONE OR SOFT TISSUE INJURY

    Directory of Open Access Journals (Sweden)

    Amitabh Das

    2014-10-01

    Full Text Available Fat embolism syndrome (FES, without evidence of bone or soft tissue injury is uncommon, and in absence of validated diagnostic criteria, its diagnosis is mainly dependent on treating clinician, who should have high index of suspicion. Treatment is predominantly supportive, and apart from some mortality, recovery is generally seen. Present article is a case report of a boy who suffered blunt injury due to fall from height, had no objective evidence of bone or soft tissue injury, but diagnosed as a case of fat embolism syndrome, using Gurd-Wilson and Schonfeld’s criteria, treated by pulmonary support and aggressive resuscitation, but he died after 4 days of admission to hospital.

  6. Iron supplementation at high altitudes induces inflammation and oxidative injury to lung tissues in rats

    Energy Technology Data Exchange (ETDEWEB)

    Salama, Samir A., E-mail: salama.3@buckeyemail.osu.edu [High Altitude Research Center, Taif University, Al-Haweiah, Taif 21974 (Saudi Arabia); Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Cairo 11751 (Egypt); Department of Pharmacology and GTMR Unit, College of Clinical Pharmacy, Taif University, Al-Haweiah, Taif 21974 (Saudi Arabia); Omar, Hany A. [Department of Pharmacology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514 (Egypt); Maghrabi, Ibrahim A. [Department of Clinical Pharmacy, College of Clinical Pharmacy, Taif University, Al-Haweiah, Taif 21974 (Saudi Arabia); AlSaeed, Mohammed S. [Department of Surgery, College of Medicine, Taif University, Al-Haweiah, Taif 21974 (Saudi Arabia); EL-Tarras, Adel E. [High Altitude Research Center, Taif University, Al-Haweiah, Taif 21974 (Saudi Arabia)

    2014-01-01

    Exposure to high altitudes is associated with hypoxia and increased vulnerability to oxidative stress. Polycythemia (increased number of circulating erythrocytes) develops to compensate the high altitude associated hypoxia. Iron supplementation is, thus, recommended to meet the demand for the physiological polycythemia. Iron is a major player in redox reactions and may exacerbate the high altitudes-associated oxidative stress. The aim of this study was to explore the potential iron-induced oxidative lung tissue injury in rats at high altitudes (6000 ft above the sea level). Iron supplementation (2 mg elemental iron/kg, once daily for 15 days) induced histopathological changes to lung tissues that include severe congestion, dilatation of the blood vessels, emphysema in the air alveoli, and peribronchial inflammatory cell infiltration. The levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α), lipid peroxidation product and protein carbonyl content in lung tissues were significantly elevated. Moreover, the levels of reduced glutathione and total antioxidant capacity were significantly reduced. Co-administration of trolox, a water soluble vitamin E analog (25 mg/kg, once daily for the last 7 days of iron supplementation), alleviated the lung histological impairments, significantly decreased the pro-inflammatory cytokines, and restored the oxidative stress markers. Together, our findings indicate that iron supplementation at high altitudes induces lung tissue injury in rats. This injury could be mediated through excessive production of reactive oxygen species and induction of inflammatory responses. The study highlights the tissue injury induced by iron supplementation at high altitudes and suggests the co-administration of antioxidants such as trolox as protective measures. - Highlights: • Iron supplementation at high altitudes induced lung histological changes in rats. • Iron induced oxidative stress in lung tissues of rats at high altitudes. • Iron

  7. Association between traumatic bone marrow abnormalities of the knee, the trauma mechanism and associated soft-tissue knee injuries

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Nicole [University Hospital Zurich, Institute of Diagnostic and Interventional Radiology, Zurich (Switzerland); University of Zurich, Department of Forensic Medicine and Radiology, Institute of Forensic Medicine, Zurich (Switzerland); Andreisek, Gustav; Karer, Anissja T.; Manoliu, Andrei; Ulbrich, Erika J. [University Hospital Zurich, Institute of Diagnostic and Interventional Radiology, Zurich (Switzerland); Bouaicha, Samy [University Hospital Zurich, Department of Trauma Surgery, Zurich (Switzerland); Naraghi, Ali [University of Toronto, Department of Medical Imaging, Mount Sinai Hospital and the University Health Network, Toronto, ON (Canada); Seifert, Burkhardt [University of Zurich, Epidemiology, Biostatistics and Prevention Institute, Department of Biostatistics, Zurich (Switzerland)

    2017-01-15

    To determine the association between traumatic bone marrow abnormalities, the knee injury mechanism, and associated soft tissue injuries in a larger cohort than those in the published literature. Retrospective study including 220 patients with traumatic knee injuries. Knee MRIs were evaluated for trauma mechanism, soft tissue injury, and the location of bone marrow abnormalities. The locations of the abnormalities were correlated with trauma mechanisms and soft tissue injuries using the chi-square test with Bonferroni correction. One hundred and forty-four valgus injuries, 39 pivot shift injuries, 25 lateral patellar dislocations, 8 hyperextensions, and 4 dashboard injuries were included. Valgus and pivot shift injuries showed traumatic bone marrow abnormalities in the posterolateral regions of the tibia. Abnormalities after patellar dislocation were found in the anterolateral and centrolateral femur and patella. Hyperextension injuries were associated with abnormalities in almost all regions, and dashboard injuries were associated with changes in the anterior regions of the tibia and femur. Our study provides evidence of associations between traumatic bone marrow abnormality patterns and different trauma mechanisms in acute knee injury, and reveals some overlap, especially of the two most common trauma mechanisms (valgus and pivot shift), in a large patient cohort. (orig.)

  8. Tissue plasminogen activator-mediated fibrinolysis protects against axonal degeneration and demyelination after sciatic nerve injury.

    Science.gov (United States)

    Akassoglou, K; Kombrinck, K W; Degen, J L; Strickland, S

    2000-05-29

    Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin and can trigger the degradation of extracellular matrix proteins. In the nervous system, under noninflammatory conditions, tPA contributes to excitotoxic neuronal death, probably through degradation of laminin. To evaluate the contribution of extracellular proteolysis in inflammatory neuronal degeneration, we performed sciatic nerve injury in mice. Proteolytic activity was increased in the nerve after injury, and this activity was primarily because of Schwann cell-produced tPA. To identify whether tPA release after nerve damage played a beneficial or deleterious role, we crushed the sciatic nerve of mice deficient for tPA. Axonal demyelination was exacerbated in the absence of tPA or plasminogen, indicating that tPA has a protective role in nerve injury, and that this protective effect is due to its proteolytic action on plasminogen. Axonal damage was correlated with increased fibrin(ogen) deposition, suggesting that this protein might play a role in neuronal injury. Consistent with this idea, the increased axonal degeneration phenotype in tPA- or plasminogen-deficient mice was ameliorated by genetic or pharmacological depletion of fibrinogen, identifying fibrin as the plasmin substrate in the nervous system under inflammatory axonal damage. This study shows that fibrin deposition exacerbates axonal injury, and that induction of an extracellular proteolytic cascade is a beneficial response of the tissue to remove fibrin. tPA/plasmin-mediated fibrinolysis may be a widespread protective mechanism in neuroinflammatory pathologies.

  9. Negative pressure wound therapy for soft tissue injuries around the foot and ankle

    Directory of Open Access Journals (Sweden)

    Oh Jong-Keon

    2009-05-01

    Full Text Available Abstract Background This study was performed to evaluate the results of negative pressure wound therapy (NPWT in patients with open wounds in the foot and ankle region. Materials and methods Using a NPWT device, 16 patients were prospectively treated for soft tissue injuries around the foot and ankle. Mean patient age was 32.8 years (range, 3–67 years. All patients had suffered an acute trauma, due to a traffic accident, a fall, or a crush injury, and all had wounds with underlying tendon or bone exposure. Necrotic tissues were debrided before applying NPWT. Dressings were changed every 3 or 4 days and treatment was continued for 18.4 days on average (range, 11–29 days. Results Exposed tendons and bone were successfully covered with healthy granulation tissue in all cases except one. The sizes of soft tissue defects reduced from 56.4 cm2 to 42.9 cm2 after NPWT (mean decrease of 24%. In 15 of the 16 cases, coverage with granulation tissue was achieved and followed by a skin graft. A free flap was needed to cover exposed bone and tendon in one case. No major complication occurred that was directly attributable to treatment. In terms of minor complications, two patients suffered scar contracture of grafted skin. Conclusion NPWT was found to facilitate the rapid formation of healthy granulation tissue on open wounds in the foot and ankle region, and thus, to shorten healing time and minimize secondary soft tissue defect coverage procedures.

  10. Role of endogenous Schwann cells in tissue repair after spinal cord injury

    Institute of Scientific and Technical Information of China (English)

    Shu-xin Zhang; Fengfa Huang; Mary Gates; Eric G. Holmberg

    2013-01-01

    Schwann cells are glial cells of peripheral nervous system, responsible for axonal myelination and ensheathing, as well as tissue repair following a peripheral nervous system injury. They are one of several cell types that are widely studied and most commonly used for cell transplantation to treat spinal cord injury, due to their intrinsic characteristics including the ability to secrete a variety of neurotrophic factors. This mini review summarizes the recent findings of endogenous Schwann cells after spinal cord injury and discusses their role in tissue repair and axonal regeneration. After spinal cord injury, numerous endogenous Schwann cells migrate into the lesion site from the nerve roots, involving in the construction of newly formed repaired tissue and axonal myelination. These invading Schwann cells also can move a long distance away from the injury site both rostrally and caudally. In addition, Schwann cells can be induced to migrate by minimal insults (such as scar ablation) within the spinal cord and integrate with astrocytes under certain circumstances. More importantly, the host Schwann cells can be induced to migrate into spinal cord by transplantation of different cell types, such as exogenous Schwann cells, olfactory ensheathing cells, and bone marrow-derived stromal stem cells. Migration of endogenous Schwann cells following spinal cord injury is a common natural phenomenon found both in animal and human, and the myelination by Schwann cells has been examined effective in signal conduction electrophysiologically. Therefore, if the inherent properties of endogenous Schwann cells could be developed and utilized, it would offer a new avenue for the restoration of injured spinal cord.

  11. Protective effects of acupuncture on brain tissue following ischemia/reperfusion injury

    Institute of Scientific and Technical Information of China (English)

    Mingshan Wang; Fuguo Ma; Huailong Chen

    2008-01-01

    BACKGROUND: In patients with cerebrovascular disease, by means of the neuroendocrine system, acupuncture supports the transformation of a local pathological status into a physiological status. Recently, great progress has been made in studying the protective effects of acupuncture on brain ischemia/reperfusion injury. OBJECTIVE: To summarize research advances in the protective effects of acupuncture on brain ischemia/reperfusion injury. RETRIEVAL STRATEGY: Using the terms "acupuncture, transcutaneous electrical acupoint stimulation, cerebral ischemia/reperfusion injury, and cerebral protection", we retrieved articles from the PubMed database published between January 1991 and June 1994. Meanwhile, we searched the China National Knowledge Infrastructure with the same terms. Altogether, 114 articles and their results were analyzed. Inclusive criteria: studies that were closely related to the protective effects of acupuncture on brain ischemia/reperfusion injury, or studies, whose contents were in the same study field and were published recently, or in the authorized journals. Exclusive criteria: repetitive studies. LITERATURE EVALUATION: Thirty articles that related to the protective effects of acupuncture on brain ischemia/reperfusion injury were included. Among them, 7 were clinical studies, and the remaining 23 articles were animal experimental studies. DATA SYNTHESIS: ① Animal experimental studies have demonstrated that acupuncture improves brain blood perfusion and brain electrical activity, influences pathomorphological and ultramicrostructural changes in ischemic brain tissue, is beneficial in maintaining the stability of intracellular and extracellular ions, resists free radical injury and lipid peroxidation, and influences cytokine, neurotransmitter, brain cell signal transduction, and apoptosis-regulating genes. ② Clinical studies have demonstrated that acupuncture not only promotes nutritional supply to local brain tissue in patients with cerebral

  12. Statins in nephrotic syndrome: a new weapon against tissue injury.

    Science.gov (United States)

    Buemi, Michele; Nostro, Lorena; Crascì, Eleonora; Barillà, Antonio; Cosentini, Vincenzo; Aloisi, Carmela; Sofi, Tito; Campo, Susanna; Frisina, Nicola

    2005-11-01

    The nephrotic syndrome is characterized by metabolic disorders leading to an increase in circulating lipoproteins levels. Hypertriglyceridemia and hypercholesterolemia in this case may depend on a reduction in triglyceride-rich lipoproteins catabolism and on an increase in hepatic synthesis of Apo B-containing lipoproteins. These alterations are the starting point of a self-maintaining mechanism, which can accelerate the progression of chronic renal failure. Indeed, hyperlipidemia can affect renal function, increase proteinuria and speed glomerulosclerosis, thus determining a higher risk of progression to dialysis. 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is the rate-limiting enzyme in cholesterol synthesis from mevalonate and its inhibitors, or statins, can therefore interfere with the above-mentioned consequences of hyperlipidemia. Statins are already well known for their effectiveness on primary cardiovascular prevention, which cannot be explained only through their hypolipemic effect. As far as kidney diseases are concerned, statin therapy has been shown to prevent creatinine clearance decline and to slow renal function loss, particularly in case of proteinuria, and its favorable effect may depend only partially on the attenuation of hyperlipidemia. Statins may therefore confer tissue protection through lipid-independent mechanisms, which can be triggered by other mediators, such as angiotensin receptor blockers. Possible pathways for the protective action of statins, other than any hypocholesterolemic effect, are: cellular apoptosis/proliferation balance, inflammatory cytokines production, and signal transduction regulation. Statins also play a role in the regulation of the inflammatory and immune response, coagulation process, bone turnover, neovascularization, vascular tone, and arterial pressure. In this study, we would like to provide scientific evidences for the pleiotropic effects of statins, which could be the starting point for the

  13. Tissues Use Resident Dendritic Cells and Macrophages to Maintain Homeostasis and to Regain Homeostasis upon Tissue Injury: The Immunoregulatory Role of Changing Tissue Environments

    Directory of Open Access Journals (Sweden)

    Maciej Lech

    2012-01-01

    Full Text Available Most tissues harbor resident mononuclear phagocytes, that is, dendritic cells and macrophages. A classification that sufficiently covers their phenotypic heterogeneity and plasticity during homeostasis and disease does not yet exist because cell culture-based phenotypes often do not match those found in vivo. The plasticity of mononuclear phagocytes becomes obvious during dynamic or complex disease processes. Different data interpretation also originates from different conceptual perspectives. An immune-centric view assumes that a particular priming of phagocytes then causes a particular type of pathology in target tissues, conceptually similar to antigen-specific T-cell priming. A tissue-centric view assumes that changing tissue microenvironments shape the phenotypes of their resident and infiltrating mononuclear phagocytes to fulfill the tissue's need to maintain or regain homeostasis. Here we discuss the latter concept, for example, why different organs host different types of mononuclear phagocytes during homeostasis. We further discuss how injuries alter tissue environments and how this primes mononuclear phagocytes to enforce this particular environment, for example, to support host defense and pathogen clearance, to support the resolution of inflammation, to support epithelial and mesenchymal healing, and to support the resolution of fibrosis to the smallest possible scar. Thus, organ- and disease phase-specific microenvironments determine macrophage and dendritic cell heterogeneity in a temporal and spatial manner, which assures their support to maintain and regain homeostasis in whatever condition. Mononuclear phagocytes contributions to tissue pathologies relate to their central roles in orchestrating all stages of host defense and wound healing, which often become maladaptive processes, especially in sterile and/or diffuse tissue injuries.

  14. 皮下埋线治疗慢性软组织损伤53例%53 cases of chronic soft tissue injury using subcutaneous line embedding therapy

    Institute of Scientific and Technical Information of China (English)

    叶立汉; 胡亚明

    2003-01-01

    @@ INTRODUCTION Acupuncture and ventouse can dredge meridian, activate blood circulation and relieve pain thus is effective to chronic soft tissue injury.We investigated a kind of method using subcutaneous line-embedding therapy to cure chronic soft tissue injury and explore a high performance and effective method in curing chronic soft tissue injury.

  15. Treatment of Calcaneal Fracture With Severe Soft Tissue Injury and Osteomyelitis: A Case Report.

    Science.gov (United States)

    Karns, Michael; Dailey, Steven K; Archdeacon, Michael T

    2015-01-01

    Advancements in surgical technique have resulted in the ability to reconstruct lower extremity injuries that would have previously been treated by amputation. Currently, a paucity of data is available specifically addressing limb amputation versus reconstruction for calcaneal fractures with severe soft tissue compromise. Reconstruction leaves the patient with their native limb; however, multiple surgeries, infections, chronic pain, and a poor functional outcome are very real possibilities. We present the case of a complex calcaneal fracture complicated by soft tissue injury and osteomyelitis that highlights the importance of shared decision-making between patient and surgeon when considering reconstruction versus amputation. This case exemplifies the need for open communication concerning the risks and benefits of treatment modalities while simultaneously considering the patient's expectations and desired outcomes.

  16. Nerve autografts and tissue-engineered materials for the repair of peripheral nerve injuries: a 5-year bibliometric analysis

    Directory of Open Access Journals (Sweden)

    Yuan Gao

    2015-01-01

    Full Text Available With advances in biomedical methods, tissue-engineered materials have developed rapidly as an alternative to nerve autografts for the repair of peripheral nerve injuries. However, the materials selected for use in the repair of peripheral nerve injuries, in particular multiple injuries and large-gap defects, must be chosen carefully. Various methods and materials for protecting the healthy tissue and repairing peripheral nerve injuries have been described, and each method or material has advantages and disadvantages. Recently, a large amount of research has been focused on tissue-engineered materials for the repair of peripheral nerve injuries. Using the keywords "pe-ripheral nerve injury", "autotransplant", "nerve graft", and "biomaterial", we retrieved publications using tissue-engineered materials for the repair of peripheral nerve injuries appearing in the Web of Science from 2010 to 2014. The country with the most total publications was the USA. The institutions that were the most productive in this field include Hannover Medical School (Germany, Washington University (USA, and Nantong University (China. The total number of publications using tissue-engineered materials for the repair of peripheral nerve injuries grad-ually increased over time, as did the number of Chinese publications, suggesting that China has made many scientific contributions to this field of research.

  17. The Immune System in Tissue Environments Regaining Homeostasis after Injury: Is “Inflammation” Always Inflammation?

    OpenAIRE

    2016-01-01

    Inflammation is a response to infections or tissue injuries. Inflammation was once defined by clinical signs, later by the presence of leukocytes, and nowadays by expression of “proinflammatory” cytokines and chemokines. But leukocytes and cytokines often have rather anti-inflammatory, proregenerative, and homeostatic effects. Is there a need to redefine “inflammation”? In this review, we discuss the functions of “inflammatory” mediators/regulators of the innate immune system that determine t...

  18. Can measurement of apparent diffusion coefficient before treatment predict the response to uterine artery embolization for adenomyosis?

    Energy Technology Data Exchange (ETDEWEB)

    Park, Yaewon; Kim, Man Deuk; Jung, Dae Chul; Lee, Shin Jae; Kim, Gyoungmin; Park, Sung Il; Won, Jong Yun; Lee, Do Yun [Yonsei University College of Medicine, Department of Radiology, Severance Hospital, Seoul (Korea, Republic of)

    2015-05-01

    To determine the usefulness of the apparent diffusion coefficient (ADC) in predicting response to uterine artery embolization (UAE) for symptomatic adenomyosis. A prospective study was performed on 25 patients who underwent diffusion-weighted (DW) magnetic resonance imaging (MRI) before UAE between June 2011 and December 2012. All patients underwent 3-month follow-up MRI after UAE using non-spherical polyvinyl alcohol (PVA) particles ranging from 150 to 500 μm. Quantitative measurement of the ADC was performed for each adenomyosis. Complete response and incomplete response were defined as ≥90 % or <90 %, respectively, of the non-perfusion area with adenomyosis at the follow-up MRI. The ADC values were compared between patients who achieved complete or incomplete response which was assessed according to the MRI findings after UAE. Nineteen patients showed complete response, and six showed incomplete response. The ADC value ranged from 0.842 to 1.346 x 10{sup -3} mm{sup 2}/s (mean 1.075 ± 0.117). The mean ADC was 1.043 ± 0.237 in the complete response group and 1.176 ± 0.429 in the incomplete response group (0.012). Using a threshold of <1.147 x 10{sup -3} mm{sup 2}/s, the sensitivity and specificity of the ADC to predict success after UAE were 83.3 % and 84.2 %. The ADC of adenomyosis may potentially predict a successful response to UAE for adenomyosis. (orig.)

  19. TREATMENT OF 120 CASES OF SOFT-TISSUE INJURY OF THE SHOULDER-BACK REGION WITH FLOATING NEEDLE

    Institute of Scientific and Technical Information of China (English)

    WANG Jian-ping; WANG Ke-hong; WANG Xiu-juan

    2005-01-01

    @@ In recent several years, the authors of the present paper employed floating needle to treat 120 cases of soft tissue injury of the shoulder-back region and achieved a good therapeutic effect. Following is the report.

  20. Metals detected by ICP/MS in wound tissue of war injuries without fragments in Gaza

    Directory of Open Access Journals (Sweden)

    Barbieri Maurizio

    2010-06-01

    Full Text Available Abstract Background The amount and identity of metals incorporated into "weapons without fragments" remain undisclosed to health personnel. This poses a long-term risk of assumption and contributes to additional hazards for victims because of increased difficulties with clinical management. We assessed if there was evidence that metals are embedded in "wounds without fragments" of victims of the Israeli military operations in Gaza in 2006 and 2009. Methods Biopsies of "wounds without fragments" from clinically classified injuries, amputation (A, charred (C, burns (B, multiple piercing wounds by White Phosphorus (WP (M, were analyzed by ICP/MS for content in 32 metals. Results Toxic and carcinogenic metals were detected in folds over control tissues in wound tissues from all injuries: in A and C wounds (Al, Ti, Cu, Sr, Ba, Co, Hg, V, Cs and Sn, in M wounds (Al, Ti, Cu, Sr, Ba, Co and Hg and in B wounds (Co, Hg, Cs, and Sn; Pb and U in wounds of all classes; B, As, Mn, Rb, Cd, Cr, Zn in wounds of all classes, but M; Ni was in wounds of class A. Kind and amounts of metals correlate with clinical classification of injuries, exposing a specific metal signature, similar for 2006 and 2009 samples. Conclusions The presence of toxic and carcinogenic metals in wound tissue is indicative of the presence in weapon inducing the injury. Metal contamination of wounds carries unknown long term risks for survivors, and can imply effects on populations from environmental contamination. We discuss remediation strategies, and believe that these data suggest the need for epidemiological and environmental surveys.

  1. Tissue reconstruction of skin failures and soft-tissue injuries using regenerative medicine methods

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    Natalia V. Smirnova

    2016-12-01

    Full Text Available The electrospinning technique has been used to manufacture a composite material based on nanofibers made of aliphatic copolyamide and composite nanofibers made of chitosan and chitin nanofibrils. Experimental in vivo studies of the designed material as wound covering were carried out to treat a vast and multilayered wound on a rat's back. After 28days and nights of observation, complete epithelialization of the wound surface was established to occur in the experimental rat group. Histological analysis of scar tissue showed the presence of a small minority of capillaries and a low amount of infiltrating cells. The survival of animals was 100%. At the same time, in the control group of animals, lethality was observed in 11% of cases, and suppurative complications were observed in 100% of cases. Thrombocyte gel prepared from the peripheral blood of the patients was used to increase the rate of tissue regeneration, and to reduce the infection probability. The curative effect was proved to increase when wound dressings and autologous blood product separation, i.e., thrombocyte gel and platelet-rich plasma, were applied simultaneously.

  2. Autologous Skin Cell Spray for Massive Soft Tissue War Injuries: A Prospective, Case-Control, Multicenter Trial

    Science.gov (United States)

    2015-01-01

    AD______________ AWARD NUMBER: W81XWH-13-2-0031 TITLE: Autologous Skin Cell Spray for Massive Soft Tissue War Injuries: A Prospective, Case...DATES COVERED 15Mar2013-31Oct2014 4. TITLE AND SUBTITLE Autologous Skin Cell Spray for Massive Soft Tissue War Injuries: A Prospective, Case-Control...assess the success of skin cell spray combined with a biocomposite subcutaneous (INTEGRA) layer for repair of large open wounds. The objective is the

  3. Bone And Soft Tissue Changes In Patients With Spinal Cord Injury And Multiple Sclerosis

    Directory of Open Access Journals (Sweden)

    Dionyssiotis Yannis

    2014-12-01

    Full Text Available In patients with spinal cord injury and multiple sclerosis, deterioration of body composition (changes in bone, fat and muscle mass is associated with increased risk for diseases such as coronary artery heart disease, non-insulin dependent diabetes mellitus, lipid metabolism abnormalities, and osteoporotic fractures in these patients. Immobility leads to a changing pattern of loading in the paralyzed areas, and secondary alteration in structure. However, bone and soft tissue changes in these patients are usually neglected. The purpose of this article is to update on the pathophysiological mechanisms leading to bone and soft tissue changes, and to increase the awareness of the treating physicians with respect to bone, muscle and fat loss and their consequences aiming to obtain measures to prevent bone and soft tissue loss in these patients.

  4. Staphylococcus aureus Panton-Valentine leukocidin contributes to inflammation and muscle tissue injury.

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    Ching Wen Tseng

    Full Text Available Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA threatens public health worldwide, and epidemiologic data suggest that the Panton-Valentine Leukocidin (PVL expressed by most CA-MRSA strains could contribute to severe human infections, particularly in young and immunocompetent hosts. PVL is proposed to induce cytolysis or apoptosis of phagocytes. However, recent comparisons of isogenic CA-MRSA strains with or without PVL have revealed no differences in human PMN cytolytic activity. Furthermore, many of the mouse studies performed to date have failed to demonstrate a virulence role for PVL, thereby provoking the question: does PVL have a mechanistic role in human infection? In this report, we evaluated the contribution of PVL to severe skin and soft tissue infection. We generated PVL mutants in CA-MRSA strains isolated from patients with necrotizing fasciitis and used these tools to evaluate the pathogenic role of PVL in vivo. In a model of necrotizing soft tissue infection, we found PVL caused significant damage of muscle but not the skin. Muscle injury was linked to induction of pro-inflammatory chemokines KC, MIP-2, and RANTES, and recruitment of neutrophils. Tissue damage was most prominent in young mice and in those strains of mice that more effectively cleared S. aureus, and was not significant in older mice and mouse strains that had a more limited immune response to the pathogen. PVL mediated injury could be blocked by pretreatment with anti-PVL antibodies. Our data provide new insights into CA-MRSA pathogenesis, epidemiology and therapeutics. PVL could contribute to the increased incidence of myositis in CA-MRSA infection, and the toxin could mediate tissue injury by mechanisms other than direct killing of phagocytes.

  5. The Effectiveness of Physical Agents for Lower-Limb Soft Tissue Injuries: A Systematic Review.

    Science.gov (United States)

    Yu, Hainan; Randhawa, Kristi; Côté, Pierre; Optima Collaboration

    2016-07-01

    Study Design Systematic review. Background Soft tissue injuries to the lower limb bring a substantial health and economic burden to society. Physical agents are commonly used to treat these injuries. However, the effectiveness of many such physical agents is not clearly established in the literature. Objective To evaluate the effectiveness and safety of physical agents for soft tissue injuries of the lower limb. Methods We searched 5 databases from 1990 to 2015 for randomized controlled trials (RCTs), cohort studies, and case-control studies. Paired reviewers independently screened the retrieved literature and appraised relevant studies using the Scottish Intercollegiate Guidelines Network criteria. Studies with a high risk of bias were excluded. We synthesized low-risk-of-bias studies according to principles of best-evidence synthesis. Results We screened 10261 articles. Of 43 RCTs identified, 20 had a high risk of bias and were excluded from the analysis, and 23 RCTs had a low risk of bias and were included in the analysis. The available higher-quality evidence suggests that patients with persistent plantar fasciitis may benefit from ultrasound or foot orthoses, while those with persistent midportion Achilles tendinopathy may benefit from shockwave therapy. However, the current evidence does not support the use of shockwave therapy for recent plantar fasciitis, low-Dye taping for persistent plantar fasciitis, low-level laser therapy for recent ankle sprains, or splints for persistent midportion Achilles tendinopathy. Finally, evidence on the effectiveness of the following interventions is not established in the current literature: (1) shockwave therapy for persistent plantar fasciitis, (2) cryotherapy or assistive devices for recent ankle sprains, (3) braces for persistent midportion Achilles tendinopathy, and (4) taping or electric muscle stimulation for patellofemoral pain syndrome. Conclusion Almost half the identified RCTs that evaluated the effectiveness of

  6. Interleukin-19 mediates tissue damage in murine ischemic acute kidney injury.

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    Yu-Hsiang Hsu

    Full Text Available Inflammation and renal tubular injury are major features of acute kidney injury (AKI. Many cytokines and chemokines are released from injured tubular cells and acts as proinflammatory mediators. However, the role of IL-19 in the pathogenesis of AKI is not defined yet. In bilateral renal ischemia/reperfusion injury (IRI-induced and HgCl2-induced AKI animal models, real-time quantitative (RTQ-PCR showed that the kidneys, livers, and lungs of AKI mice expressed significantly higher IL-19 and its receptors than did sham control mice. Immunohistochemical staining showed that IL-19 and its receptors were strongly stained in the kidney, liver, and lung tissue of AKI mice. In vitro, IL-19 upregulated MCP-1, TGF-β1, and IL-19, and induced mitochondria-dependent apoptosis in murine renal tubular epithelial M-1 cells. IL-19 upregulated TNF-α and IL-10 in cultured HepG2 cells, and it increased IL-1β and TNF-α expression in cultured A549 cells. In vivo, after renal IRI or a nephrotoxic dose of HgCl2 treatment, IL-20R1-deficient mice (the deficiency blocks IL-19 signaling showed lower levels of blood urea nitrogen (BUN in serum and less tubular damage than did wild-type mice. Therefore, we conclude that IL-19 mediates kidney, liver, and lung tissue damage in murine AKI and that blocking IL-19 signaling may provide a potent therapeutic strategy for treating AKI.

  7. Elective amputation and bionic substitution restore functional hand use after critical soft tissue injuries

    Science.gov (United States)

    Aszmann, Oskar C.; Vujaklija, Ivan; Roche, Aidan D.; Salminger, Stefan; Herceg, Malvina; Sturma, Agnes; Hruby, Laura A.; Pittermann, Anna; Hofer, Christian; Amsuess, Sebastian; Farina, Dario

    2016-01-01

    Critical soft tissue injuries may lead to a non-functional and insensate limb. In these cases standard reconstructive techniques will not suffice to provide a useful outcome, and solutions outside the biological arena must be considered and offered to these patients. We propose a concept which, after all reconstructive options have been exhausted, involves an elective amputation along with a bionic substitution, implementing an actuated prosthetic hand via a structured tech-neuro-rehabilitation program. Here, three patients are presented in whom this concept has been successfully applied after mutilating hand injuries. Clinical tests conducted before, during and after the procedure, evaluating both functional and psychometric parameters, document the benefits of this approach. Additionally, in one of the patients, we show the possibility of implementing a highly functional and natural control of an advanced prosthesis providing both proportional and simultaneous movements of the wrist and hand for completing tasks of daily living with substantially less compensatory movements compared to the traditional systems. It is concluded that the proposed procedure is a viable solution for re-gaining highly functional hand use following critical soft tissue injuries when existing surgical measures fail. Our results are clinically applicable and can be extended to institutions with similar resources. PMID:27721419

  8. A CD11d Monoclonal Antibody Treatment Reduces Tissue Injury and Improves Neurological Outcome after Fluid Percussion Brain Injury in Rats

    OpenAIRE

    2012-01-01

    Traumatic brain injury (TBI) is an international health concern often resulting in chronic neurological abnormalities, including cognitive deficits, emotional disturbances, and motor impairments. An anti-CD11d monoclonal antibody that blocks the CD11d/CD18 integrin and vascular cell adhesion molecule (VCAM)-1 interaction following experimental spinal cord injury improves functional recovery, while reducing the intraspinal number of neutrophils and macrophages, oxidative activity, and tissue d...

  9. Oxidative stress and DNA damage signalling in skeletal muscle in pressure-induced deep tissue injury.

    Science.gov (United States)

    Sin, Thomas K; Pei, Xiao M; Teng, Bee T; Tam, Eric W; Yung, Benjamin Y; Siu, Parco M

    2013-02-01

    The molecular mechanisms that contribute to the pathogenesis of pressure-induced deep tissue injury are largely unknown. This study tested the hypothesis that oxidative stress and DNA damage signalling mechanism in skeletal muscle are involved in deep tissue injury. Adult Sprague Dawley rats were subject to an experimental protocol to induce deep tissue injury. Two compression cycles with a static pressure of 100 mmHg was applied to an area of 1.5 cm(2) over the mid-tibialis region of right limb of the rats. The left uncompressed limb served as intra-animal control. Muscle tissues underneath compression region were collected for examination. Our analyses indicated that pathohistological characteristics including rounding contour of myofibres and extensive nuclei accumulation were apparently shown in compressed muscles. The elevation of 8OHdG immunopositively stained nuclei indicated the presence of oxidative DNA damage. Increase in oxidative stress was revealed by showing significant elevation of 4HNE and decreases in mRNA abundance of SOD1, catalase and GPx, and protein content of SOD2 in compressed muscles relative to control muscles. Increase in nitrosative stress was demonstrated by significant elevation of nitrotyrosine and NOS2 mRNA content. The activation of tumor suppressor p53 signalling was indicated by the remarkable increases in protein contents of total p53 and serine-15 phosphorylated p53. The transcript expression of the DNA-repairing enzyme, Rad23A, was significantly suppressed in compressed muscles. Our time-course study indicated that increased oxidative/nitrosative stress and proapoptotic signalling were maintained in muscles receiving increasing amount of compression cycles and post-compression time. Furthermore, resveratrol was found to attenuate the histological damage, oxidative/nitrosative stress and proapoptotic signalling in response to prolonged moderate compression. In conclusion, our findings are consistent with the hypothesis that

  10. Reduction of thermocoagulative injury via use of a picosecond infrared laser (PIRL) in laryngeal tissues.

    Science.gov (United States)

    Böttcher, Arne; Kucher, Stanislav; Knecht, Rainald; Jowett, Nathan; Krötz, Peter; Reimer, Rudolph; Schumacher, Udo; Anders, Sven; Münscher, Adrian; Dalchow, Carsten V; Miller, R J Dwayne

    2015-04-01

    The carbon dioxide (CO2) laser is routinely used in glottic microsurgery for the treatment of benign and malignant disease, despite significant collateral thermal damage secondary to photothermal vaporization without thermal confinement. Subsequent tissue response to thermal injury involves excess collagen deposition resulting in scarring and functional impairment. To minimize collateral thermal injury, short-pulse laser systems such as the microsecond pulsed erbium:yttrium-aluminium-garnet (Er:YAG) laser and picosecond infrared laser (PIRL) have been developed. This study compares incisions made in ex vivo human laryngeal tissues by CO2 and Er:YAG lasers versus PIRL using light microscopy, environmental scanning electron microscopy (ESEM), and infrared thermography (IRT). In comparison to the CO2 and Er:YAG lasers, PIRL incisions showed significantly decreased mean epithelial (59.70 µm) and subepithelial (22.15 µm) damage zones (p lasers (p laser incisions. IRT demonstrated median temperature rise of 4.1 K in PIRL vocal fold incisions, significantly less than for Er:YAG laser cuts (171.85 K; p lasers in human glottis and supraglottic tissues.

  11. Liver regeneration - The best kept secret: A model of tissue injury response

    Directory of Open Access Journals (Sweden)

    Javier A. Cienfuegos

    2014-03-01

    Full Text Available Liver regeneration (LR is one of the most amazing tissue injury response. Given its therapeutic significance has been deeply studied in the last decades. LR is an extraordinary complex process, strictly regulated, which accomplishes the characteristics of the most evolutionary biologic systems (robustness and explains the difficulties of reshaping it with therapeutic goals. TH reproduces the physiological tissue damage response pattern, with a first phase of priming of the hepatocytes -cell-cycle transition G0-G1-, and a second phase of proliferation -cell-cycle S/M phases- which ends with the liver mass recovering. This process has been related with the tissue injury response regulators as: complement system, platelets, inflammatory cytokines (TNF-α, IL-1β, IL-6, growth factors (HGF, EGF, VGF and anti-inflammatory factors (IL-10, TGF-β. Given its complexity and strict regulation, illustrates the unique alternative to liver failure is liver transplantation. The recent induced pluripotential cells (iPS description and the mesenchymal stem cell (CD133+ plastic capability have aroused new prospects in the cellular therapy field. Those works have assured the cooperation between mesenchymal and epithelial cells. Herein, we review the physiologic mechanisms of liver regeneration.

  12. Pressure Combined with Ischemia/Reperfusion Injury Induces Deep Tissue Injury via Endoplasmic Reticulum Stress in a Rat Pressure Ulcer Model.

    Science.gov (United States)

    Cui, Fei-Fei; Pan, Ying-Ying; Xie, Hao-Huang; Wang, Xiao-Hui; Shi, Hong-Xue; Xiao, Jian; Zhang, Hong-Yu; Chang, Hao-Teng; Jiang, Li-Ping

    2016-02-25

    Pressure ulcer is a complex and significant health problem in long-term bedridden patients, and there is currently no effective treatment or efficient prevention method. Furthermore, the molecular mechanisms and pathogenesis contributing to the deep injury of pressure ulcers are unclear. The aim of the study was to explore the role of endoplasmic reticulum (ER) stress and Akt/GSK3β signaling in pressure ulcers. A model of pressure-induced deep tissue injury in adult Sprague-Dawley rats was established. Rats were treated with 2-h compression and subsequent 0.5-h release for various cycles. After recovery, the tissue in the compressed regions was collected for further analysis. The compressed muscle tissues showed clear cellular degenerative features. First, the expression levels of ER stress proteins GRP78, CHOP, and caspase-12 were generally increased compared to those in the control. Phosphorylated Akt and phosphorylated GSK3β were upregulated in the beginning of muscle compression, and immediately significantly decreased at the initiation of ischemia-reperfusion injury in compressed muscles tissue. These data show that ER stress may be involved in the underlying mechanisms of cell degeneration after pressure ulcers and that the Akt/GSK3β signal pathway may play an important role in deep tissue injury induced by pressure and ischemia/reperfusion.

  13. Recovery of renal function after administration of adipose-tissue-derived stromal vascular fraction in rat model of acute kidney injury induced by ischemia/reperfusion injury.

    Science.gov (United States)

    Lee, Chunwoo; Jang, Myoung Jin; Kim, Bo Hyun; Park, Jin Young; You, Dalsan; Jeong, In Gab; Hong, Jun Hyuk; Kim, Choung-Soo

    2017-03-10

    Acute kidney injury (AKI) induced by ischemia/reperfusion (I/R) injury is a major challenge in critical care medicine. The purpose of this study is to determine the therapeutic effects of the adipose-tissue-derived stromal vascular fraction (SVF) and the optimal route for SVF delivery in a rat model of AKI induced by I/R injury. Fifty male Sprague-Dawley rats were randomly divided into five groups (10 animals per group): sham, nephrectomy control, I/R injury control, renal arterial SVF infusion and subcapsular SVF injection. To induce AKI by I/R injury, the left renal artery was clamped with a nontraumatic vascular clamp for 40 min, and the right kidney was removed. Rats receiving renal arterial infusion of SVF had a significantly reduced increase in serum creatinine compared with the I/R injury control group at 4 days after I/R injury. The glomerular filtration rate of the renal arterial SVF infusion group was maintained at a level similar to that of the sham and nephrectomy control groups at 14 days after I/R injury. Masson's trichrome staining showed significantly less fibrosis in the renal arterial SVF infusion group compared with that in the I/R injury control group in the outer stripe (P renal arterial SVF infusion and subcapsular SVF injection groups compared with the I/R injury control group in the outer stripe (P renal function is effectively rescued from AKI induced by I/R injury through the renal arterial administration of SVF in a rat model.

  14. Heat transfer analysis of catheters used for localized tissue cooling to attenuate reperfusion injury.

    Science.gov (United States)

    Merrill, Thomas L; Mitchell, Jennifer E; Merrill, Denise R

    2016-08-01

    Recent revascularization success for ischemic stroke patients using stentrievers has created a new opportunity for therapeutic hypothermia. By using short term localized tissue cooling interventional catheters can be used to reduce reperfusion injury and improve neurological outcomes. Using experimental testing and a well-established heat exchanger design approach, the ɛ-NTU method, this paper examines the cooling performance of commercially available catheters as function of four practical parameters: (1) infusion flow rate, (2) catheter location in the body, (3) catheter configuration and design, and (4) cooling approach. While saline batch cooling outperformed closed-loop autologous blood cooling at all equivalent flow rates in terms of lower delivered temperatures and cooling capacity, hemodilution, systemic and local, remains a concern. For clinicians and engineers this paper provides insights for the selection, design, and operation of commercially available catheters used for localized tissue cooling.

  15. Effect of mild hypothermia on glucose metabolism and glycerol of brain tissue in patients with severe traumatic brain injury

    Institute of Scientific and Technical Information of China (English)

    WANG Qiong; LI Ai-lin; ZHI Da-shi; HUANG Hui-ling

    2007-01-01

    Objective:To study the effect of mild hypothermia on glucose metabolism and glycerol of brain tissue in patients with severe traumatic brain injury (STBI) using clinical microdialysis.Methods: Thirty-one patients with STBI ( GCS ≤8) were randomly divided into hypothermic group (Group A) and control group (Group B). Microdialysis catheters were inserted into the cerebral cortex of perilesional and normal brain tissue. All samples were analyzed using CMA microdialysis analyzer.Results: In comparison with the control group, lactate/glucose ratio ( L/G) , lactate/pyruvate ratio ( L/P) and glycerol (Gly) in perilensional tissue were significantly decreased; L/P in normal brain tissue was significantly decreased. In control group, L/G, L/P and Gly in perilensional tissue were higher than that in normal brain tissue. In the hypothermic group, L/P in perilensional tissue was higher than that in relative normal brain.Conclusions: Mild hypothermia protects brain tissues by decreasing L/G, L/P and Gly in perilensional tissue and L/P in "normal brain" tissues. The energy crisis and membrane phospholipid degradation in perilensional tissue are easier to happen after traumatic brain injury, and mild hypothermia protects brain better in perilensional tissue than in normal brain tissue.

  16. Diannexin protects against renal ischemia reperfusion injury and targets phosphatidylserines in ischemic tissue.

    Directory of Open Access Journals (Sweden)

    Kimberley E Wever

    Full Text Available Renal ischemia/reperfusion injury (IRI frequently complicates shock, renal transplantation and cardiac and aortic surgery, and has prognostic significance. The translocation of phosphatidylserines to cell surfaces is an important pro-inflammatory signal for cell-stress after IRI. We hypothesized that shielding of exposed phosphatidylserines by the annexin A5 (ANXA5 homodimer Diannexin protects against renal IRI. Protective effects of Diannexin on the kidney were studied in a mouse model of mild renal IRI. Diannexin treatment before renal IRI decreased proximal tubule damage and leukocyte influx, decreased transcription and expression of renal injury markers Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 and improved renal function. A mouse model of ischemic hind limb exercise was used to assess Diannexin biodistribution and targeting. When comparing its biodistribution and elimination to ANXA5, Diannexin was found to have a distinct distribution pattern and longer blood half-life. Diannexin targeted specifically to the ischemic muscle and its affinity exceeded that of ANXA5. Targeting of both proteins was inhibited by pre-treatment with unlabeled ANXA5, suggesting that Diannexin targets specifically to ischemic tissues via phosphatidylserine-binding. This study emphasizes the importance of phosphatidylserine translocation in the pathophysiology of IRI. We show for the first time that Diannexin protects against renal IRI, making it a promising therapeutic tool to prevent IRI in a clinical setting. Our results indicate that Diannexin is a potential new imaging agent for the study of phosphatidylserine-exposing organs in vivo.

  17. Decidual transformation in adenomyosis during pregnancy as an indication for hysterectomy

    Directory of Open Access Journals (Sweden)

    Jovanović Božidar

    2009-01-01

    Full Text Available Introduction. Incidence of adenomyosis is estimated on 20% in all of extracted uteruses. Clinically, it usually exists asymptomatically, but could be followed with dysmenorrhoea, menorrhagia and pelvic pain. One third of patients with adenomyosis are sterile, however in other two thirds, conception and pregnancy could have normal development, with delivery without complications. One of possible complications related to adenomyosis is rupture of uterus during delivery. Case report. A pregnant woman at the end of the 10th lunar month of pregnancy, showed minimal labour contractions, following amnion rupture and the delivery was terminated by section ceasar. Subtotal hysterectomy was performed because of concominant profuse bleeding. Myometrium was occupied with decidual cells in trabecular arrangement, among which elongated endometrial glands were placed. Surrounding muscle fascicles showed atrophic, apoptic, and occasionally necrotic changes. Myometrial stroma was edematous, and infiltrated with mesenchymal cells, as well as with adipose cells, which were extending toward serosa. Discussion. The patient had been receiving treatment, for a long period, because of sterility without clear etiological factor. A small number of similar cases ends with complications. Here, it is evident that abundant decidual transformation of stromal cells in adenomiosis leads to atrophy and necrosis of muscle cells. Teh reduction of uterine muscle mass causes atony and threating rupture, through separation of muscle cells, and therefore the absence of their synchronized contractions. Conclusion. Atony of uterine muscle could be caused by decidual transformation in adenomyosal fields, atrophy of muscle fascicles, edema of the stroma, mesenchymal transformation, and fibrosis. This leads to a decrease in myometrial contractions, and prolonged postpartal bleeding.

  18. An acellular biologic scaffold does not regenerate appreciable de novo muscle tissue in rat models of volumetric muscle loss injury.

    Science.gov (United States)

    Aurora, Amit; Roe, Janet L; Corona, Benjamin T; Walters, Thomas J

    2015-10-01

    Extracellular matrix (ECM) derived scaffolds continue to be investigated for the treatment of volumetric muscle loss (VML) injuries. Clinically, ECM scaffolds have been used for lower extremity VML repair; in particular, MatriStem™, a porcine urinary bladder matrix (UBM), has shown improved functional outcomes and vascularization, but limited myogenesis. However, efficacy of the scaffold for the repair of traumatic muscle injuries has not been examined systematically. In this study, we demonstrate that the porcine UBM scaffold when used to repair a rodent gastrocnemius musculotendinous junction (MTJ) and tibialis anterior (TA) VML injury does not support muscle tissue regeneration. In the MTJ model, the scaffold was completely resorbed without tissue remodeling, suggesting that the scaffold may not be suitable for the clinical repair of muscle-tendon injuries. In the TA VML injury, the scaffold remodeled into a fibrotic tissue and showed functional improvement, but not due to muscle fiber regeneration. The inclusion of physical rehabilitation also did not improve functional response or tissue remodeling. We conclude that the porcine UBM scaffold when used to treat VML injuries may hasten the functional recovery through the mechanism of scaffold mediated functional fibrosis. Thus for appreciable muscle regeneration, repair strategies that incorporate myogenic cells, vasculogenic accelerant and a myoconductive scaffold need to be developed.

  19. Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue.

    Science.gov (United States)

    Terzi, Alpaslan; Iraz, Mustafa; Sahin, Semsettin; Ilhan, Atilla; Idiz, Nuri; Fadillioglu, Ersin

    2004-09-01

    Rotenone, an insecticide of botanical origin, causes toxicity through inhibition of complex I of the respiratory chain in mitochondria. This study was undertaken to determine whether rotenone-induced liver oxidant injury is prevented by erdosteine, a mucolytic agent showing antioxidant properties. There were four groups of Male Wistar Albino rats: group one was untreated as control; the other groups were treated with erdosteine (50 mg/kg per day, orally), rotenone (2.5 mg/mL once and 1 mL/kg per day for 60 days, i.p.) or rotenone plus erdosteine, respectively. Rotenone treatment without erdosteine increased xanthine oxidase (XO) enzyme activity and also increased lipid peroxidation in liver tissue (P erdosteine produced a significant decrease in lipid peroxidation and XO activities in comparison with rotenone group (P Erdosteine treatment with rotenone led to an increase in catalase (CAT) and superoxide dismutase (SOD) activities in comparison with the rotenone group (P erdosteine group, there was a negative correlation between XO activity and NO level in liver tissue (r = -0.833, P erdosteine may be a protective agent for rotenone-induced liver oxidative injury in rats.

  20. Liver transplantation in the mouse: Insights into liver immunobiology, tissue injury, and allograft tolerance.

    Science.gov (United States)

    Yokota, Shinichiro; Yoshida, Osamu; Ono, Yoshihiro; Geller, David A; Thomson, Angus W

    2016-04-01

    The surgically demanding mouse orthotopic liver transplant model was first described in 1991. It has proved to be a powerful research tool for the investigation of liver biology, tissue injury, the regulation of alloimmunity and tolerance induction, and the pathogenesis of specific liver diseases. Liver transplantation in mice has unique advantages over transplantation of the liver in larger species, such as the rat or pig, because the mouse genome is well characterized and there is much greater availability of both genetically modified animals and research reagents. Liver transplant experiments using various transgenic or gene knockout mice have provided valuable mechanistic insights into the immunobiology and pathobiology of the liver and the regulation of graft rejection and tolerance over the past 25 years. The molecular pathways identified in the regulation of tissue injury and promotion of liver transplant tolerance provide new potential targets for therapeutic intervention to control adverse inflammatory responses/immune-mediated events in the hepatic environment and systemically. In conclusion, orthotopic liver transplantation in the mouse is a valuable model for gaining improved insights into liver biology, immunopathology, and allograft tolerance that may result in therapeutic innovation in the liver and in the treatment of other diseases.

  1. Free radicals and related reactive species as mediators of tissue injury and disease: implications for Health.

    Science.gov (United States)

    Kehrer, James P; Klotz, Lars-Oliver

    2015-01-01

    A radical is any molecule that contains one or more unpaired electrons. Radicals are normal products of many metabolic pathways. Some exist in a controlled (caged) form as they perform essential functions. Others exist in a free form and interact with various tissue components. Such interactions can cause both acute and chronic dysfunction, but can also provide essential control of redox regulated signaling pathways. The potential roles of endogenous or xenobiotic-derived free radicals in several human pathologies have stimulated extensive research linking the toxicity of numerous xenobiotics and disease processes to a free radical mechanism. In recent years, improvements in analytical methodologies, as well as the realization that subtle effects induced by free radicals and oxidants are important in modulating cellular signaling, have greatly improved our understanding of the roles of these reactive species in toxic mechanisms and disease processes. However, because free radical-mediated changes are pervasive, and a consequence as well as a cause of injury, whether such species are a major cause of tissue injury and human disease remains unclear. This concern is supported by the fact that the bulk of antioxidant defenses are enzymatic and the findings of numerous studies showing that exogenously administered small molecule antioxidants are unable to affect the course of most toxicities and diseases purported to have a free radical mechanism. This review discusses cellular sources of various radical species and their reactions with vital cellular constituents, and provides examples of selected disease processes that may have a free radical component.

  2. Modelling the effect of repositioning on the evolution of skeletal muscle damage in deep tissue injury.

    Science.gov (United States)

    Demol, Jan; Deun, Dorien Van; Haex, Bart; Oosterwyck, Hans Van; Sloten, Jos Vander

    2013-04-01

    Deep tissue injury (DTI) is a localized area of tissue necrosis that originates in the subcutaneous layers under an intact skin and tends to develop when soft tissue is compressed for a prolonged period of time. In clinical practice, DTI is particularly common in bedridden patients and remains a serious issue in todays health care. Repositioning is generally considered to be an effective preventive measure of pressure ulcers. However, limited experimental research and no computational studies have been undertaken on this method. In this study, a methodology was developed to evaluate the influence of different repositioning intervals on the location, size and severity of DTI in bedridden patients. The spatiotemporal evolution of compressive stresses and skeletal muscle viability during the first 48 h of DTI onset was simulated for repositioning schemes in which a patient is turned every 2, 3, 4 or 6 h. The model was able to reproduce important experimental findings, including the morphology and location of DTI in human patients as well as the discrepancy between the internal tissue loads and the contact pressure at the interface with the environment. In addition, the model indicated that the severity and size of DTI were reduced by shortening the repositioning intervals. In conclusion, the computational framework presented in this study provides a promising modelling approach that can help to objectively select the appropriate repositioning scheme that is effective and efficient in the prevention of DTI.

  3. bFGF and TGFβ expression in rat kidneys after ischemic/ reperfusional gut injury and its relationship with tissue repair

    Institute of Scientific and Technical Information of China (English)

    Yin Hui Yang; Xiao Bing Fu; Tong Zhu Sun; Li Xian Jiang; Xiao Man Gu

    2000-01-01

    @@ INTRODUCTION Intestinal ischemia/ reperfusion ( I/ R ) occur commonly in critically ill patients. It is well recognized that gut I/R may cause tissue damage and dysfunction of intestine, and induce remote organ injury including kidney, lung, and liver[1]. It may also lead to complications after severe burn or injury. Previous studies have focused on cellular elements, cytokines and inflammatory mediators.Relatively little attention has been paid endogenous protective mechanisms, I.e. The growth factors.

  4. Influence of mirena treatment on uterine blood flow dynamics and serum related indexes of patients with uterine adenomyosis

    Institute of Scientific and Technical Information of China (English)

    Qing-Yun Wang; Hua Zhang; Zhen-Zhen Liu

    2016-01-01

    Objective:To study the influence of mirena treatment on uterine blood flow dynamics and serum related indexes of patients with uterine adenomyosis.Methods:A total of 58 patients with uterine adenomyosis who were treated in our hospital during the time from June 2014 to July 2015 were randomly divided into two groups, 29 patients in the control group were treated with danazol, and 29 patients in the observation group were treated with mirena. Then the total effective rate, adverse reaction rates, uterine blood flow dynamics indexes, serum MMP related indexes and other disease related indexes of the two groups before the treatment and at first, third and sixth month after the treatment were compared.Results:The total effective rate and adverse reaction rate of observation group were both worse than that of control group (allP0.05). While the uterine blood flow dynamics indexes of the observation group at different time after the treatment were all significantly better than those of control group (allP<0.05).Conclusions:Mirena treatment can effectively improve the uterine blood flow dynamics and serum related indexes of patients with uterine adenomyosis, and it has application value for the patients with uterine adenomyosis is higher.

  5. Effect of matrix metalloproteinase promoter polymorphisms on endometriosis and adenomyosis risk: evidence from a meta-analysis

    Indian Academy of Sciences (India)

    HUI YE; YAZHOU HE; JIARONG WANG; TIANGE SONG; ZHU LAN; YIQI ZHAO; MINGRONG XI

    2016-09-01

    Matrix metalloproteinase (MMP) promoter polymorphisms are considered to play roles in the aetiology of endometriosis and adenomyosis, however, the evidence available are inconsistent. We aimed to systematically review the asscociationbetween MMP-1 -1607 1G/2G MMP-2 -735 C/T, MMP-3 -1171 5A/6A and MMP-9 -1562 C/T polymorphisms and the risk of endometriosis and adenomyosis. A systemic search was conducted in Ovid, PubMed, Chinese National Knowledge Infrastructure and Chinese Wanfang Database. We used the pooled odds ratio (OR) and their corresponding 95% confidence interval (CI) to calculate the statistical power. Besides, we evaluated the quality of individual studies based on Newcastle–Ottawa scale. A total of 13 papers with 18 studies conformed to our inclusion criteria. We observed a significant association betweenMMP-1 -1607 1G/2G polymorphism and the susceptibility of endometriosis and adenomyosis under recessive model (OR =1.25, 95%CI = 1.03–1.53,P=0.03). While no significant association was found in MMP-2 -735 C/T, MMP-3 -1171 5A/6A and MMP-9 -1562 C/T polymorphisms. This systemic review and meta-analysis suggested that the MMP-1 -1607 1G/2G polymorphism might play an important role in the risk of endometriosis and adenomyosis. Further, more well-designed and large-scale studies regarding gene–gene and gene–environment interactions are needed in the future.

  6. A new therapeutic strategy for lung tissue injury induced by influenza with CR2 targeting complement inhibitior

    Directory of Open Access Journals (Sweden)

    Tomlinson Stephen

    2010-02-01

    Full Text Available Abstract Background Influenza is a respiratory disease that seriously threatens human health. In fact, influenza virus itself does not make critical contribution to mortality induced by influenza, but "cytokine storm" produced by the excessive immune response triggered by the virus can result in inflammatory reaction of lung tissues and fatal lung tissue injury, and thus increase influenza mortality. Therefore, besides antiviral drugs, immunosuppression drugs should also be included in infection treatment. Presentation of the hypothesis Complement is the center of inflammatory reaction. If complement system is over activated, the body will have strong inflammatory reaction or tissue injury, resulting in pathological process. Many studies have proved that, inflammatory injury of lung tissues caused by influenza virus is closely related to complement activation. Therefore, inhibiting complement activation can significantly reduce inflammatory injury in lung tissues. As complement is both a physiological defense and pathological damage medium, systematic inhibition may result in side effects including infection. Therefore, we design targeting complement inhibitors for complement activation sites, i.e. with CR2 as targeting vector, complement inhibitors like CD59 and Crry are targeted to inflammatory sites to specially inhibit the complement activation in local injury, thus local inflammatory reaction is inhibited. Testing the hypothesis CR2-CD59 and CR2-Crry targeting complement inhibitors are fusion-expressed, and their biological activity is examined via in vivo and in vitro tests. CR2 targeting complement inhibitors are used to treat mouse influenza viral pneumonia model, with PBS treatment group as the control. The survival and lung tissue injury of the mice is observed and the effect of CR2 targeting complement inhibitors on pneumonia induced by influenza virus is evaluated. Implications of the hypothesis CR2 targeting complement inhibitors

  7. Visualizing the neutrophil response to sterile tissue injury in mouse dermis reveals a three-phase cascade of events.

    Science.gov (United States)

    Ng, Lai Guan; Qin, Jim S; Roediger, Ben; Wang, Yilin; Jain, Rohit; Cavanagh, Lois L; Smith, Adrian L; Jones, Cheryl A; de Veer, Michael; Grimbaldeston, Michele A; Meeusen, Els N; Weninger, Wolfgang

    2011-10-01

    Neutrophil granulocytes traffic into sites of organ injury in which they may not only participate in tissue repair and pathogen clearance but may also contribute to collateral cell damage through the release of noxious mediators. The dynamics and mechanisms of neutrophil migration in the extravascular space toward loci of tissue damage are not well understood. Here, we have used intravital multi-photon microscopy to dissect the behavior of neutrophils in response to tissue injury in the dermis of mice. We found that, following confined physical injury, initially rare scouting neutrophils migrated in a directional manner toward the damage focus. This was followed by the attraction of waves of additional neutrophils, and finally stabilization of the neutrophil cluster around the injury. Although neutrophil migration in the steady state and during the scouting phase depended on pertussis toxin-sensitive signals, the amplification phase was sensitive to interference with the cyclic adenosine diphosphate ribose pathway. We finally demonstrated that neutrophil scouts also transit through the non-inflamed dermis, suggesting immunosurveillance function by these cells. Together, our data unravel a three-step cascade of events that mediates the specific accumulation of neutrophils at sites of sterile tissue injury in the interstitial space.

  8. Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma

    Science.gov (United States)

    Chen, Hsin-Yi; Chou, Hsiu-Chuan; Chang, Shing-Jyh; Liao, En-Chi; Tsai, Yi-Ting; Wei, Yu-Shan; Li, Ji-Min; Lin, Li-Hsun; Lin, Meng-Wei; Chen, Ying-Jen; Chen, Yu-Sheng; Lin, Chih-Chun; Wang, Yi-Shiuan; Ko, Mei-Lan; Chan, Hong-Lin

    2017-01-01

    Glaucoma is a group of eye diseases that can cause vision loss and optical nerve damage. To investigate the protein expression alterations in various intraocular tissues (i.e., the cornea, conjunctiva, uvea, retina, and sclera) during ischemia–reperfusion (IR) injury, this study performed a proteomic analysis to qualitatively investigate such alterations resulting from acute glaucoma. The IR injury model combined with the proteomic analysis approach of two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to monitor the protein expression alterations in two groups of specimens (an IR injury group and a control group). The analysis results revealed 221 unique differentially expressed proteins of a total of 1481 proteins in the cornea between the two groups. In addition, 97 of 1206 conjunctival proteins, 90 of 1354 uveal proteins, 61 of 1180 scleral proteins, and 37 of 1204 retinal proteins were differentially expressed. These findings imply that different ocular tissues have different tolerances against IR injury. To sum up, this study utilized the acute glaucoma model combined with 2D-DIGE and MALDI-TOF MS to investigate the IR injury affected protein expression on various ocular tissues, and based on the ratio of protein expression alterations, the alterations in the ocular tissues were in the following order: the cornea, conjunctiva, uvea, sclera, and retina. PMID:28165428

  9. 子宫腺肌病的保守治疗%Conservative Treatment of Adenomyosis

    Institute of Scientific and Technical Information of China (English)

    葛军; 韩耀伟; 周莉君

    2014-01-01

    近年来,子宫腺肌病的发病率有逐年上升趋势,临床表现具有多样化的特点。该病治疗方法包括高强度聚焦超声治疗、药物治疗、子宫切除手术、子宫动脉栓塞治疗、放置左炔诺孕酮宫内缓释系统(LNG-IUS,商品名:曼月乐)、子宫内膜剥除术等。药物治疗近期效果明显,但是停药后易复发;切除子宫往往导致患者丧失生育能力;子宫动脉栓塞治疗操作难度大,影响疗效因素多;放置LNG-IUS具有不用手术、患者依从性好的优点,但是有部分患者会有点滴出血等问题;子宫内膜剥除术效果肯定,但是不良反应相对较多,因此各种治疗方法均存在一定局限性。随着患者对保留子宫的期望值越来越高,临床应用的很多治疗子宫腺肌病的保守方法,已经成为目前临床医生普遍关心的问题。综述子宫腺肌病的保守治疗。%In recent years, the incidence of adenomyosis has shown a rising trend year by year and diversified clinical manifestations have been observed. Treatments for adenomyosis include high intensity focused ultrasound drug therapy, hysterectomy, uterine artery embolization, levonorgestrel releasing intrauterine system (LNG-IUS), endometrial stripping technique, etc. Recently, the effect of drug treatment is obvious, but it is easy to relapse after drug withdrawal. Removal of the uterus often leads to the loss of fertility. Uterine artery embolization is difficult to operate with a lot of factors influencing the curative effect; LNG-IUS had the characteristics of surgery-free and good compliance, but some patients may have the problems such as bleeding, etc.;Endometrial stripping operation has confirmable effect, but the side effect is also relatively bigger;therefore, various treatment methods all have some limitations. The conservative method to be employed in women with adenomyosis has become the topic that clinicians generally care about

  10. State of the art. Four easy pieces: interconnections between tissue injury, intermediary metabolism, autoimmunity, and chronic degeneration.

    Science.gov (United States)

    Steinman, Lawrence

    2006-08-01

    Four questions are posed: (1) Can tissue damage itself provoke autoimmunity? (2) Can genetic mutations of key structures produce tissue pathology and thus provoke autoimmunity? (3) Can acute immune damage produce tissue degeneration without further hallmarks of an immune response? (4) Can intermediary metabolism modulate immune damage to tissues? Four answers are given: (1) Tissue injury itself may lead to autoimmunity. Both innate and adaptive immunity may arise as a response to tissue injury, and the immune attack can further damage tissue. (2) Genetic mutations can lead to an immune response indistinguishable from autoimmunity, exemplified from Duchenne's Muscular Dystrophy and X-linked adrenoleukodystrophy. (3) Chronic immune damage may lead to tissue degeneration, with or without further hallmarks of an immune response. Variations on this theme, including inverse scenarios, are also possible: Inborn errors of metabolism may lead to tissue damage that may provoke an adaptive and or innate immune response. The immune response might further damage tissue. (4) Finally, perturbations of intermediary metabolism may modulate the immune response, controlling the extent of immune-mediated damage. Examples are taken from perturbations in the cholesterol pathway that influence the characteristics of the immune response, and with tryptophan metabolites that modulate autoimmunity and graft rejection. Inflammatory, degenerative, and autoimmune neurological disease will be discussed in terms of their implications for pathogenic mechanisms underlying chronic obstructive pulmonary disease.

  11. Tissue injuries of wistar rats treated with hydroalcoholic extract of Sonchus oleraceus L.

    Directory of Open Access Journals (Sweden)

    Franciele Carla Prichoa

    2011-09-01

    Full Text Available The use of plant species is emerging as an important alternative in the treatment of injuries. Therefore, the extract of Sonchus oleraceus 10% was employed in the repair of skin lesions. A total of 36 male Wistar rats were subjected to a punch injury and divided into three groups: a negative control, receiving no treatment, a positive control, treated with Dersani, and the experimental group treated with the extract. The injury was assessed macroscopically and microscopically. Morphometric data was collected at the 3rd, 5th and 7th postoperative day, and the experimental group showed greater changes in shrinkage of the lesion compared to control groups. On the 3rd postoperative day, the injury in the experimental group showed less necrotic tissue, lower slough and more granulation tissue in relation to the positive control group. On the 7th and 10th postoperative day, the injury in the experimental group showed lower slough compared to the positive control group. Microscopic analysis of lesions on the 5th postoperative day revealed increased fibroplasia in the experimental group compared to control groups, while on the 14th postoperative day less neovascularization was evident in the experimental group and increased formation of hair follicles in the negative control group. The extract of S. oleraceus provided tissue repair in accordance with normal physiological patterns thus confirming empirical evidence for its use.O emprego de espécies vegetais vem surgindo como alternativa no tratamento de lesões. Dessa forma, foi utilizado o extrato hidroalcoólico de Sonchus oleraceus a 10% na reparação de lesões cutâneas. Trinta e seis ratos machos Wistar, foram submetidos a uma lesão com "punch" e distribuídos em três grupos: controle negativo, não recebeu tratamento; controle positivo, tratado com Dersani; e o experimental, tratado com extrato. A lesão foi avaliada macroscopicamente e microscopicamente. Os dados morfométricos mostraram que

  12. Periostin as a tissue and urinary biomarker of renal injury in type 2 diabetes mellitus.

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    Bancha Satirapoj

    Full Text Available Improving the early detection of diabetic nephropathy remains a great challenge in disease management. Periostin is a marker of renal tubular injury and related to progressive kidney injury in animal models of chronic kidney disease. The clinical implications of urinary periostin activities in patients with type 2 diabetes have not been evaluated.Urine samples were obtained from 30 healthy volunteers and 328 type 2 diabetic patients with normoalbuminuria (n=114, microalbuminuria (n=100 and macroalbuminuria (n=114. The excretion levels of urinary periostin were quantified with enzyme-linked immunosorbent assay. Immunohistochemical periostin expression was determined in kidney tissues from overt diabetic nephropathy.Increased periostin expression in glomeruli and tubular epithelium in diabetic renal pathology was observed. Urinary periostin levels were significantly elevated in the patients of the normoalbuminuria [3.06 (IQR: 1.12, 6.77 ng/mgCr], microalbuminuria [8.71 (IQR: 5.09, 19.29 ng/mgCr] and macroalbuminuria [13.58 (IQR: 3.99, 16.19 ng/mgCr] compared with healthy controls [1.15 (IQR: 0.60, 1.63 ng/mgCr] (P<0.01.Increased urine periostin level significantly correlated with aging, high albuminuria and decline of GFR. Urine periostin ELISA also demonstrated high performance for the diagnosis of established normoalbuminuric, microalbuminuric and macroalbuminuric type 2 diabetes (AUC 0.78 (95%CI, 0.71 to 0.86, 0.99 (95%CI, 0.98 to 1.00 and 0.95 (95%CI, 0.91 to 0.98, respectively.The study indicates that increased urine periostin levels can be detected in patients with type 2 diabetes before the onset of significant albuminuria. Urinary periostin is an associated renal derangement in patients with established diabetic nephropathy and it may be used as an early marker of diabetic renal injury.

  13. Brown adipose tissue and its modulation by a mitochondria-targeted peptide in rat burn injury-induced hypermetabolism.

    Science.gov (United States)

    Yo, Kikuo; Yu, Yong-Ming; Zhao, Gaofeng; Bonab, Ali A; Aikawa, Naoki; Tompkins, Ronald G; Fischman, Alan J

    2013-02-15

    Hypermetabolism is a prominent feature of burn injury, and altered mitochondria function is presumed to contribute to this state. Recently, brown adipose tissue (BAT) was found to be present not only in rodents but also in humans, and its activity is associated with resting metabolic rate. In this report, we elucidate the relationship between burn injury-induced hypermetabolism and BAT activity and the possible role of the mitochondria-targeted peptide SS31 in attenuating burn injury-induced hypermetabolism by using a rat burn injury model. We demonstrate that burn injury induces morphological changes in interscapular BAT (iBAT). Burn injury was associated with iBAT activation, and this effect was positively correlated with increased energy expenditure. BAT activation was associated with augmentation of mitochondria biogenesis, and UCP1 expression in the isolated iBAT mitochondria. In addition, the mitochondria-targeted peptide SS31 attenuated burn injury-induced hypermetabolism, which was accompanied by suppression of UCP1 expression in isolated mitochondria. Our results suggest that BAT plays an important role in burn injury-induced hypermetabolism through its morphological changes and expression of UCP1.

  14. Effect of Fucoidan Extracted from Mozuku on Experimental Cartilaginous Tissue Injury 

    Directory of Open Access Journals (Sweden)

    Saburo Minami

    2012-11-01

    Full Text Available We investigated the effect of fucoidan, a sulfated polysaccharide, on acceleration of healing of experimental cartilage injury in a rabbit model. An injured cartilage model was surgically created by introduction of three holes, one in the articular cartilage of the medial trochlea and two in the trochlear sulcus of the distal femur. Rabbits in three experimental groups (F groups were orally administered fucoidan of seven different molecular weights (8, 50, 146, 239, 330, 400, or 1000 kD for 3 weeks by screening. Control (C group rabbits were provided water ad libitum. After the experimental period, macroscopic examination showed that the degree of filling in the fucoidan group was higher than that in the C group. Histologically, the holes were filled by collagen fiber and fibroblasts in the C group, and by chondroblasts and fibroblasts in the F groups. Image analysis of Alcian blue- and safranin O-stained F-group specimens showed increased production of glycosaminoglycans (GAGs and proteoglycans (PGs, respectively. Some injured holes were well repaired both macroscopically and microscopically and were filled with cartilage tissues; cartilage matrices such as PGs and GAGs were produced in groups F 50, F 146, and F 239. Thus, fucoidan administration enhanced morphologically healing of cartilage injury.

  15. Uterine adenomyosis in infertile patients: MR imaging findings and clinical conclusions; MRT der uterinen Adenomyose bei infertilen Patientinnen: Ergebnisse und klinische Rueckschluesse

    Energy Technology Data Exchange (ETDEWEB)

    Zangos, S.; Eichler, K.; Herzog, C.; Marquardt, F.; Peters, J.; Jacobi, V.; Mack, M.G.; Vogl, T.J. [Inst. fuer Diagnostische und Interventionelle Radiologie, J.W. Goethe-Univ. Frankfurt (Germany); Kissler, S.; Mueller, A.; Siebzehnruebl, E.; Kaufmann, M. [Klinik fuer Gynaekologie und Geburtshilfe, J. W. Goethe-Univ. Frankfurt (Germany); Hamscho, N.; Gruenwald, F. [Klinik fuer Nuklearmedizin, J. W. Goethe-Univ. Frankfurt (Germany)

    2004-11-01

    Purpose: To evaluate the clinical value of magnetic resonance imaging (MRI) of the uterus and the pelvis in infertile female with symptomatic endometriosis for the assessment of uterine morphology and function. Materials and Methods: Forty-one infertile women (mean age: 33.2 years) with symptomatic endometriosis documented by laparoscopy were evaluated with highfield MRI (Sonata, Siemens) using T2- and T1-weighted sequences. In addition, the patients underwent hysterosalpingoscintigraphy (HSSG) for evaluation of the uterotubal transport capacity. Pathologic findings of the uterine junction zone were correlated with the laparoscopic results, clinical symptoms and uterotubal transport capacity. Results: Adenomyosis was diagnosed on T2-weighted MR-images in 35 (85.4%) patients (focal adenomatosis in 26 patients and diffuse adenomatosis in 9). Patients showing signs of adenomyosis tended to be older than patients without adenomyosis (mean age 34.1 years vs. 30.1 years) and showed a longer history of symptomatic endometriosis. A positive transport capacity in HSSG was observed in 73.1% (19/26) of the patients with focal adenomyosis and only 22.2% (2/9) of patients with diffuse adenomyosis. In 83.3% (5/6) of the patients without signs of adenomyosis, a positive uterotubal transport was documented. Conclusion: MRI of the uterus is a helpful diagnostic tool for the diagnosis of adenomyosis and for planning further therapies. Patients with the diagnosis of adenomyosis showed a reduced uterotubal transport capacity that depended on the severity of adenomyotic changes and might be a possible cause of infertility. (orig.)

  16. Brain tissue oxygen tension and its response to physiological manipulations: influence of distance from injury site in a swine model of traumatic brain injury.

    Science.gov (United States)

    Hawryluk, Gregory W J; Phan, Nicolas; Ferguson, Adam R; Morabito, Diane; Derugin, Nikita; Stewart, Campbell L; Knudson, M Margaret; Manley, Geoffrey; Rosenthal, Guy

    2016-11-01

    OBJECTIVE The optimal site for placement of tissue oxygen probes following traumatic brain injury (TBI) remains unresolved. The authors used a previously described swine model of focal TBI and studied brain tissue oxygen tension (PbtO2) at the sites of contusion, proximal and distal to contusion, and in the contralateral hemisphere to determine the effect of probe location on PbtO2 and to assess the effects of physiological interventions on PbtO2 at these different sites. METHODS A controlled cortical impact device was used to generate a focal lesion in the right frontal lobe in 12 anesthetized swine. PbtO2 was measured using Licox brain tissue oxygen probes placed at the site of contusion, in pericontusional tissue (proximal probe), in the right parietal region (distal probe), and in the contralateral hemisphere. PbtO2 was measured during normoxia, hyperoxia, hypoventilation, and hyperventilation. RESULTS Physiological interventions led to expected changes, including a large increase in partial pressure of oxygen in arterial blood with hyperoxia, increased intracranial pressure (ICP) with hypoventilation, and decreased ICP with hyperventilation. Importantly, PbtO2 decreased substantially with proximity to the focal injury (contusion and proximal probes), and this difference was maintained at different levels of fraction of inspired oxygen and partial pressure of carbon dioxide in arterial blood. In the distal and contralateral probes, hypoventilation and hyperventilation were associated with expected increased and decreased PbtO2 values, respectively. However, in the contusion and proximal probes, these effects were diminished, consistent with loss of cerebrovascular CO2 reactivity at and near the injury site. Similarly, hyperoxia led to the expected rise in PbtO2 only in the distal and contralateral probes, with little or no effect in the proximal and contusion probes, respectively. CONCLUSIONS PbtO2 measurements are strongly influenced by the distance from the

  17. Perivascular adipose tissue-secreted angiopoietin-like protein 2 (Angptl2) accelerates neointimal hyperplasia after endovascular injury.

    Science.gov (United States)

    Tian, Zhe; Miyata, Keishi; Tazume, Hirokazu; Sakaguchi, Hisashi; Kadomatsu, Tsuyoshi; Horio, Eiji; Takahashi, Otowa; Komohara, Yoshihiro; Araki, Kimi; Hirata, Yoichiro; Tabata, Minoru; Takanashi, Shuichiro; Takeya, Motohiro; Hao, Hiroyuki; Shimabukuro, Michio; Sata, Masataka; Kawasuji, Michio; Oike, Yuichi

    2013-04-01

    Much attention is currently focused on the role of perivascular adipose tissue in development of cardiovascular disease (CVD). Some researchers view it as promoting CVD through secretion of cytokines and growth factors called adipokines, while recent reports reveal that perivascular adipose tissue can exert a protective effect on CVD development. Furthermore, adiponectin, an anti-inflammatory adipokine, reportedly suppresses neointimal hyperplasia after endovascular injury, whereas such vascular remodeling is enhanced by pro-inflammatory adipokines secreted by perivascular adipose, such as tumor necrosis factor-α (TNF-α). These findings suggest that extent of vascular remodeling, a pathological process associated with CVD development, depends on the balance between pro- and anti-inflammatory adipokines secreted from perivascular adipose tissue. We previously demonstrated that angiopoietin-like protein 2 (Angptl2), a pro-inflammatory factor secreted by adipose tissue, promotes adipose tissue inflammation and subsequent systemic insulin resistance in obesity. Here, we examined whether Angptl2 secreted by perivascular adipose tissue contributes to vascular remodeling after endovascular injury in studies of transgenic mice expressing Angptl2 in adipose tissue (aP2-Angptl2 transgenic mice) and Angptl2 knockout mice (Angptl2(-/-) mice). To assess the role of Angptl2 secreted by perivascular adipose tissue on vascular remodeling after endovascular injury, we performed adipose tissue transplantation experiments using these mice. Wild-type mice with perivascular adipose tissue derived from aP2-Angptl2 mice exhibited accelerated neointimal hyperplasia after endovascular injury compared to wild-type mice transplanted with wild-type tissue. Conversely, vascular inflammation and neointimal hyperplasia after endovascular injury were significantly attenuated in wild-type mice transplanted with Angptl2(-/-) mouse-derived perivascular adipose tissue compared to wild-type mice

  18. A Computational, Tissue-Realistic Model of Pressure Ulcer Formation in Individuals with Spinal Cord Injury.

    Science.gov (United States)

    Ziraldo, Cordelia; Solovyev, Alexey; Allegretti, Ana; Krishnan, Shilpa; Henzel, M Kristi; Sowa, Gwendolyn A; Brienza, David; An, Gary; Mi, Qi; Vodovotz, Yoram

    2015-06-01

    People with spinal cord injury (SCI) are predisposed to pressure ulcers (PU). PU remain a significant burden in cost of care and quality of life despite improved mechanistic understanding and advanced interventions. An agent-based model (ABM) of ischemia/reperfusion-induced inflammation and PU (the PUABM) was created, calibrated to serial images of post-SCI PU, and used to investigate potential treatments in silico. Tissue-level features of the PUABM recapitulated visual patterns of ulcer formation in individuals with SCI. These morphological features, along with simulated cell counts and mediator concentrations, suggested that the influence of inflammatory dynamics caused simulations to be committed to "better" vs. "worse" outcomes by 4 days of simulated time and prior to ulcer formation. Sensitivity analysis of model parameters suggested that increasing oxygen availability would reduce PU incidence. Using the PUABM, in silico trials of anti-inflammatory treatments such as corticosteroids and a neutralizing antibody targeted at Damage-Associated Molecular Pattern molecules (DAMPs) suggested that, at best, early application at a sufficiently high dose could attenuate local inflammation and reduce pressure-associated tissue damage, but could not reduce PU incidence. The PUABM thus shows promise as an adjunct for mechanistic understanding, diagnosis, and design of therapies in the setting of PU.

  19. Erdosteine protects rat testis tissue from hypoxic injury by reducing apoptotic cell death.

    Science.gov (United States)

    Guven, A; Ickin, M; Uzun, O; Bakar, C; Balbay, E Gulec; Balbay, O

    2014-02-01

    The purpose of this study was to examine the effects of hypobaric hypoxia on testis morphology and the effects of erdosteine on testis tissue. Caspase-3 and hypoxia-inducible factor 1α expressions were detected by immunohistochemistry. Adult male Wistar rats were placed in a hypobaric hypoxic chamber. Rats in the erdosteine group were exposed to the same conditions and treated orally with erdosteine (20 mg kg(-1) daily) at the same time from the first day of hypoxic exposure for 2 weeks. The normoxia group was evaluated as the control. The hypoxia group showed decreased height of spermatogenic epithelium in some seminiferous tubules, vacuolisation in spermatogenic epithelial cells, deterioration and gaps in the basal membrane and an increase in blood vessels in the interstitial area. The erdosteine group showed amelioration of both epithelial cell vacuolisation and basal membrane deterioration. Numbers of hypoxia-inducible factor 1α-immunostained Sertoli and Leydig cells were significantly higher in the hypoxia group than in the erdosteine group. The number of seminiferous tubules with caspase-3-immunostained germ cells was highest in the hypoxia group and decreased in the erdosteine and normoxia groups respectively. Based on these observations, erdosteine protects testis tissue from hypoxic injury by reducing apoptotic cell death.

  20. A Computational, Tissue-Realistic Model of Pressure Ulcer Formation in Individuals with Spinal Cord Injury.

    Directory of Open Access Journals (Sweden)

    Cordelia Ziraldo

    2015-06-01

    Full Text Available People with spinal cord injury (SCI are predisposed to pressure ulcers (PU. PU remain a significant burden in cost of care and quality of life despite improved mechanistic understanding and advanced interventions. An agent-based model (ABM of ischemia/reperfusion-induced inflammation and PU (the PUABM was created, calibrated to serial images of post-SCI PU, and used to investigate potential treatments in silico. Tissue-level features of the PUABM recapitulated visual patterns of ulcer formation in individuals with SCI. These morphological features, along with simulated cell counts and mediator concentrations, suggested that the influence of inflammatory dynamics caused simulations to be committed to "better" vs. "worse" outcomes by 4 days of simulated time and prior to ulcer formation. Sensitivity analysis of model parameters suggested that increasing oxygen availability would reduce PU incidence. Using the PUABM, in silico trials of anti-inflammatory treatments such as corticosteroids and a neutralizing antibody targeted at Damage-Associated Molecular Pattern molecules (DAMPs suggested that, at best, early application at a sufficiently high dose could attenuate local inflammation and reduce pressure-associated tissue damage, but could not reduce PU incidence. The PUABM thus shows promise as an adjunct for mechanistic understanding, diagnosis, and design of therapies in the setting of PU.

  1. Complement activation in coronary artery bypass grafting patients without cardiopulmonary bypass - The role of tissue injury by surgical incision

    NARCIS (Netherlands)

    Gu, YJ; Mariani, MA; Boonstra, PW; Grandjean, JG; van Oeveren, W

    1999-01-01

    Study objectives: Complement activation is a trigger in inducing inflammation in patients who undergo coronary artery bypass grafting (CABG) and is usually thought to be induced by the use of cardiopulmonary bypass (CPB), In this study, we examined whether tissue injury caused by chest surgical inci

  2. Profile of the subjects with soft tissue injuries attended at an occupational health service and the RSI

    Directory of Open Access Journals (Sweden)

    Camila de Freitas

    2015-07-01

    Full Text Available Aim: To investigate the profile of subjects with soft tissue injuries attended at the Reference Center of Occupational Health – CEREST in the municipality of Santos, Sao Paulo state, in 2010, and the social insurance benefits granted.Materials and Methods: Analysis of medical records of the subjects assisted at CEREST in 2010, surveying data on gender, age, occupation, clinical diagnostics, clinical complaints, retirement, etc. The clinical diagnostics were categorized according to the International Classification of Diseases - ICD-10, subjects with soft tissue injuries were selected, and the diagnostics related to mental health disorders were registered. Data were recorded in Microsoft Excel spreadsheet and analyzed using statistical software R Development Core Team.Results: Of the 206 medical records analyzed, 18.0% (n=37 showed soft tissue injuries, 81.1% were female and 18.9% were male, and the subjects’ mean age was 43.24 years (SD=8.76. Subjects between 31 and 50 years old (70.2% were the most affected. The most affected occupations were cleaners, general service workers, and bank clerks. The most prevalent clinical diagnoses were synovitis and tenosynovitis, shoulder bursitis, and rotator cuff syndrome, with 62.2% of the subjects presenting more than one clinical diagnosis. 13.5% of the subjects also presented mental disorders. Association between retirement from work and the presence of soft tissue injury was observed (p=0.032. Only 13.5% of the diagnoses had some association with the work conditions.Conclusions: The general profile of the workers with soft tissue injuries was obtained: prevalence in women, diseases manifested in productive age, difficulty of association with work conditions, need for interdisciplinary interventions.

  3. Digital image analysis of striated skeletal muscle tissue injury during reperfusion after induced ischemia

    Science.gov (United States)

    Rosero Salazar, Doris Haydee; Salazar Monsalve, Liliana

    2015-01-01

    Conditions such as surgical procedures or vascular diseases produce arterial ischemia and reperfusion injuries, which generate changes in peripheral tissues and organs, for instance, in striated skeletal muscle. To determine such changes, we conducted an experimental method in which 42 male Wistar rat were selected, to be undergone to tourniquet application on the right forelimb and left hind limb, to induce ischemia during one and three hours, followed by reperfusion periods starting at one hour and it was prolonged up to 32 days. Extensor carpi radialis longus and soleus respectively, were obtained to be processed for histochemical and morphometric analysis. By means of image processing and detection of regions of interest, variations of areas occupied by muscle fibers and intramuscular extracellular matrix (IM-ECM) throughout reperfusion were observed. In extensor carpi radialis longus, results shown reduction in the area occupied by muscle fibers; this change is significant between one hour and three hours ischemia followed by 16 hours, 48 hours and 32 days reperfusión (p˂0.005). To compare only periods of reperfusión that continued to three hours ischemia, were found significant differences, as well. For area occupied by IM-ECM, were identified increments in extensor carpi radialis longus by three hours ischemia and eight to 16 days reperfusion; in soleus, was observed difference by one hour ischemia with 42 hours reperfusion, and three hours ischemia followed by four days reperfusion (p˂0.005). Skeletal muscle develops adaptive changes in longer reperfusion, to deal with induced injury. Descriptions beyond 32 days reperfusion, can determine recovering normal pattern.

  4. Effects of recombinant sCR1 on the immune inflammatory reaction in acute spinal cord injury tissue of rats

    Institute of Scientific and Technical Information of China (English)

    李良满; 朱悦; 范广宇

    2005-01-01

    Objective: To determine the effects of recombinant soluble complement receptor type I (sCR1) on the immune inflammatory reaction in acute spinal cord injury tissue of rats and its protective effects. Results: The motor function of rat in sCR1 group at 3 d, 7 d, and 14 d was obviously better than that in NS group (P<0.01, P<0.01, P<0.01). C3c positive expression in sCR1 group at each time point after injury was obviously less than that in NS group (P<0.01). The myeloperoxidase activity in sCR1 group at each time point after injury was obviously less than that in NS group (P<0.01). Conclusions: Recombinant soluble complement receptor type I (sCR1) can lessen the immune inflammatory reaction in acute spinal cord injury tissue and relieve secondary spinal cord injury by inhibiting the activation of the complement system.

  5. Common Laundry Detergent Ingredient May Help Preserve Muscle Tissue After Severe Injury

    Science.gov (United States)

    ... browser Favorites '); document.write(' Bookmark this page on Google '); document.write(' Submit this page to Yahoo! Buzz '); ... injury—such as a crush injury from a car accident or a blast injury sustained in combat— ...

  6. Transplantation of neural progenitor cells differentiated from adipose tissue-derived stem cells for treatment of sciatic nerve injury

    Institute of Scientific and Technical Information of China (English)

    Shasha Dong§; Na Liu§; Yang Hu ; Ping Zhang; Chao Pan; Youping Zhang; Yingxin Tang; Zhouping Tang 

    2016-01-01

    Objectives: Currently, the clinical repair of sciatic nerve injury remains difficult. Previous studies have confirmed that transplantation of adipose tissue-derived stem cells promotes nerve regeneration and restoration at peripheral nerve injury sites. Methods:In this study, adipose tissue-derived stem cells were induced to differentiate into neural progenitor cells, transfected with a green fluorescent protein-containing lentivirus, and then transplanted into the lesions of rats with sciatic nerve compression injury. Results: Fluorescence microscopy revealed that the transplanted cells survived, migrated, and differentiated in rats. At two weeks post-operation, a large number of transplanted cells had migrated to the injured lesions; at six weeks post-operation, transplanted cells were visible around the injured nerve and several cells were observed to express a Schwann cell marker. Sciatic function index and electrophysiological outcomes of the transplantation group were better than those of the control group. Cell transplantation promoted the recovery of motor nerve conduction velocity and com-pound muscle action potential amplitude, and reduced gastrocnemius muscle atrophy. Conclusions: Our experimental findings indicate that neural progenitor cells, differentiated from adipose tissue-derived stem cells, are potential seed stem cells that can be transplanted into lesions to treat sciatic nerve injury. This provides a theoretical basis for their use in clinical applications.

  7. Stress analyses coupled with damage laws to determine biomechanical risk factors for deep tissue injury during sitting.

    Science.gov (United States)

    Linder-Ganz, Eran; Gefen, Amit

    2009-01-01

    Deep tissue injury (DTI) is a potentially life-threatening form of pressure ulcer that onsets in muscle tissue overlying bony prominences and progresses unnoticeably to more superficial tissues. To minimize DTI, the efficacy of wheelchair cushions should be evaluated not only based on their performance in redistributing interface pressures but also according to their effects on stress concentrations in deep tissues, particularly muscles. However, a standard bioengineering approach for such analyses is missing in literature. The goals of this study were to develop an algorithm to couple finite element (FE) modeling of the buttocks with an injury threshold for skeletal muscle and with a damage-stiffening law for injured muscle tissue, from previous animal experiments, to predict DTI onset and progression for different patient anatomies and wheelchair cushions. The algorithm was also employed for identifying intrinsic (anatomical) biomechanical risk factors for DTI onset. A set of three-dimensional FE models of seated human buttocks was developed, representing different severities of pathoanatomical changes observed in chronically sitting patients: muscle atrophy and "flattening" of the ischial tuberosity (IT). These models were then tested with cushions of different stiffnesses representing products available on the market and semirigid supports. Outcome measures were the percentage of damaged muscle tissue volumes after 90 min and 110 min of simulated continuous immobilized sitting as well as muscle injury rates post-60 min, -90 min, and -110 min of continuous sitting. Damaged muscle volumes grew exponentially with the level of muscle atrophy. For example, simulation of a subject with 70% muscle atrophy sitting on a soft cushion showed damage to 33% of the muscle volume after 90 min of immobilized sitting, whereas a comparable simulation with a nonatrophied muscle yielded only 0.4% damaged tissue volume. The rates of DTI progression also increased substantially with

  8. CXCL16 participates in pathogenesis of immunological liver injury by regulating T lymphocyte infiltration in liver tissue

    Institute of Scientific and Technical Information of China (English)

    Huan-Bin Xu; Yan-Ping Gong; Jin Cheng; Yi-Wei Chu; Si-Dong Xiong

    2005-01-01

    AIM: To investigate the role of CXCL16 in the pathogenesis of immunological liver injury and to explore the possible mechanism of T lymphocyte infiltration requlated by CXCL16.METHODS: Immunological liver injury in murine model was induced by Bacille Calmette-Guerin and lipopolysaccharide.Expression pattem and distribution of CXCL16 were examined by real-time quantitative RT-PCR and immunohistochemical analysis. Anti-CXCL16 antibody was administrated in vivo to investigate its effect on T-cell recruitment and acute hepatic necrosis. The survival of murine model was also evaluated.RESULTS: The murine immunological liver injury model was successfully established. CXCL16 expression increased and predominantly distributed in periportal areas and vascular endothelia in injured liver tissues. Administration of anti-CXCL16 Ab protected the mice from death and acute liver damage. Approximately 70% of the mice survived for 72 h in the anti-CXCL16 Ab treatment group, whereas 80% died within 72 h in control Ab group. The number of liver-infiltrating T lymphocytes was significantly reduced from 1.01×L07 to 3.52x 106/liver, compared with control Ab treatment.CONCLUSION: CXCL16 is involved in immunological liver injury by regulating T lymphocyte infiltration in liver tissue.

  9. Topical nonsteroidal anti-inflammatory drugs for the treatment of pain due to soft tissue injury: diclofenac epolamine topical patch

    OpenAIRE

    Lionberger, David R; Brennan, Michael J.

    2010-01-01

    David R Lionberger1, Michael J Brennan21Southwest Orthopedic Group, Houston, TX, USA; 2Department of Medicine, Bridgeport Hospital, Bridgeport, CT, USAAbstract: The objective of this article is to review published clinical data on diclofenac epolamine topical patch 1.3% (DETP) in the treatment of acute soft tissue injuries, such as strains, sprains, and contusions. Review of published literature on topical nonsteroidal anti-inflammatory drugs (NSAIDs), diclofenac, and DETP in patients with ac...

  10. Assessment of the Effectiveness of Extracorporeal Shock Wave Therapy (ESWT) For Soft Tissue Injuries (ASSERT): An Online Database Protocol

    OpenAIRE

    Maffulli, G; Hemmings, Steph; Maffulli, Nicola

    2014-01-01

    Background Soft tissue injuries and tendinopathies account for large numbers of chronic musculoskeletal disorders. Extracorporeal shockwave therapy (ESWT) is popular, and effective in the management of chronic tendon conditions in the elbow, shoulder, and pain at and around the heel. Methods/Design Ethical approval was granted from the South East London Research Ethics Committee to implement a database for the Assessment of Effectiveness of Extracorporeal Shock Wave Therapy for Soft Tis...

  11. Subfailure overstretch injury leads to reversible functional impairment and purinergic P2X7 receptor activation in intact vascular tissue

    Directory of Open Access Journals (Sweden)

    Weifeng Luo

    2016-09-01

    Full Text Available Vascular stretch injury is associated with blunt trauma, vascular surgical procedures, and harvest of human saphenous vein for use in vascular bypass grafting. A model of subfailure overstretch in rat abdominal aorta was developed to characterize surgical vascular stretch injury. Longitudinal stretch of rat aorta was characterized ex vivo. Stretch to the haptic endpoint where the tissues would no longer lengthen, occurred at twice the resting length. The stress produced at this length was greater than physiologic mechanical forces but well below the level of mechanical disruption. Functional responses were determined in a muscle bath and this subfailure overstretch injury led to impaired smooth muscle function that was partially reversed by treatment with purinergic receptor (P2X7R antagonists. These data suggest that vasomotor dysfunction caused by subfailure overstretch injury may be due to activation of P2X7R. These studies have implications for our understanding of mechanical stretch injury of blood vessels and offer novel therapeutic opportunities.

  12. EXPRESSION OF INTERCELLULAR ADHESION MOLECULE IN LUNG TISSUES OF EXPERIMENTAL ACUTE LUNG INJURY AND THE AFFECT OF RHUBARB ON IT

    Institute of Scientific and Technical Information of China (English)

    李春盛; 桂培春; 何新华

    2000-01-01

    Objeaive. To approach the relation and the possible mechanism between the expression of intercellular adhesion molecule (ICAM-1) mRNA and acute lung injury (ALI) and the mechanisms of rhubarb in the prevention and treatment of the lung injury. Methods. Lipopolysaeeharide (LPS) was injected into the sublingual vein of male Wistar rats to perform ALI animal model. The rats were divided into 4 groups: LPS group, control group, rhubarb group and dexamethasoue group.Macroscopic and histopathological e~aminatiom were performed and biological markers were measured for the lung specimem. The markers included lung wet/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary vascular permeability and pulmonary alveolar permeability index. Molecular hybridization method was used to determine the expression of ICAM-1 mRNA. Results. In the lung tissues, the ICAM-1 mRNA expression was increased in the endothelial cells of pulmonary veins and capillaries, rhubarb and dexamethasone had the action of decreasing the expression. The light reflex value in the gray scale scanning showed that in the comparison between the LPS and the control group, the gray scale value of the lung tissues in ALI was significantly increased, thus the light reflex value was markedly decreased (P < 0.01),demonstrating the expression of ICAM-1 mRNA was increased. In comparison with the LPS group, dexamethasoue and rhubarb emfld decrease the gray scale value of the lung tissue significantly, thus the light reflex value was elevated (P< 0.01, P < 0.05) ; the correslxmding pathologic changes of lung tissues and the biological markers of the lung injury were simifieantlv decreased or ameliorated. Conclusions. The increase of the expression d ICAM-1 mRNA in the lung tissues of ALI plays the roles in ALI.The application of rhubarb and dexamethasone can decrease the expression and ameliorate the lung damage; its mechanism is possibly via the inhibition of ICAM-1 m

  13. EXPRESSION OF INTERCELLULAR ADHESION MOLECULE IN LUNG TISSUES OF EXPERIMENTAL ACUTE LUNG INJURY AND THE AFFECT OF RHUBARB ON IT

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective. To approach the relation and the possible mechanism between the expression of intercellular adhesion molecule (ICAM-1) mRNA and acute lung injury (ALI) and the mechanisms of rhubarb in the prevention and treatment of the lung injury.Methods. Lipopolysaccharide (LPS) was injected into the sublingual vein of male Wistar rats to perform ALI animal model. The rats were divided into 4 groups: LPS group, control group, rhubarb group and dexamethasone group. Macroscopic and histopathological examinations were performed and biological markers were measured for the lung specimens. The markers included lung wet/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary vascular permeability and pulmonary alveolar permeability index. Molecular hybridization method was used to determine the expression of ICAM-1 mRNA.Results. In the lung tissues, the ICAM-1 mRNA expression was increased in the endothelial cells of pulmonary veins and capillaries, rhubarb and dexamethasone had the action of decreasing the expression. The light reflex value in the gray scale scanning showed that in the comparison between the LPS and the control group, the gray scale value of the lung tissues in ALI was significantly increased, thus the light reflex value was markedly decreased (P<0.01), demonstrating the expression of ICAM-1 mRNA was increased. In comparison with the LPS group, dexamethasone and rhubarb could decrease the gray scale value of the lung tissue significantly, thus the light reflex value was elevated (P<0.01, P<0.05); the corresponding pathologic changes of lung tissues and the biological markers of the lung injury were significantly decreased or ameliorated.Conclusions. The increase of the expression of ICAM-1 mRNA in the lung tissues of ALI plays the roles in ALI. The application of rhubarb and dexamethasone can decrease the expression and ameliorate the lung damage; its mechanism is possibly via the inhibition of ICAM

  14. Anti-human tissue factor antibody ameliorated intestinal ischemia reperfusion-induced acute lung injury in human tissue factor knock-in mice.

    Directory of Open Access Journals (Sweden)

    Xiaolin He

    Full Text Available BACKGROUND: Interaction between the coagulation and inflammation systems plays an important role in the development of acute respiratory distress syndrome (ARDS. Anti-coagulation is an attractive option for ARDS treatment, and this has promoted development of new antibodies. However, preclinical trials for these antibodies are often limited by the high cost and availability of non-human primates. In the present study, we developed a novel alternative method to test the role of a humanized anti-tissue factor mAb in acute lung injury with transgenic mice. METHODOLOGY/PRINCIPAL FINDINGS: Human tissue factor knock-in (hTF-KI transgenic mice and a novel humanized anti-human tissue factor mAb (anti-hTF mAb, CNTO859 were developed. The hTF-KI mice showed a normal and functional expression of hTF. The anti-hTF mAb specifically blocked the pro-coagulation activity of brain extracts from the hTF-KI mice and human, but not from wild type mice. An extrapulmonary ARDS model was used by intestinal ischemia-reperfusion. Significant lung tissue damage in hTF-KI mice was observed after 2 h reperfusion. Administration of CNTO859 (5 mg/kg, i.v. attenuated the severity of lung tissue injury, decreased the total cell counts and protein concentration in bronchoalveolar lavage fluid, and reduced Evans blue leakage. In addition, the treatment significantly reduced alveolar fibrin deposition, and decreased tissue factor and plasminogen activator inhibitor-1 activity in the serum. This treatment also down-regulated cytokine expression and reduced cell death in the lung. CONCLUSIONS: This novel anti-hTF antibody showed beneficial effects on intestinal ischemia-reperfusion induced acute lung injury, which merits further investigation for clinical usage. In addition, the use of knock-in transgenic mice to test the efficacy of antibodies against human-specific proteins is a novel strategy for preclinical studies.

  15. Effect of ketamine on aquaporin-4 expression and neuronal apoptosis in brain tissues following brain injury in rats

    Institute of Scientific and Technical Information of China (English)

    Zangong Zhou; Xiangyu Ji; Li Song; Jianfang Song; Shiduan Wang; Yanwei Yin

    2006-01-01

    BACKGROUND: Aquaporin-4 (AQP-4) is closely related to the formation of brain edema. Neuronal apoptosis plays an important part in the conversion of swelled neuron following traumatic brain injury. At present, the studies on the protective effect of ketamine on brain have involved in its effect on aquaporin-4 expression and neuronal apoptosis in the brain tissues following brain injury in rats.OBJECTIVE: To observe the effect of ketamine on AQP-4 expression and neuronal apoptosis in the brain tissue following rat brain injury, and analyze the time-dependence of ketamine in the treatment of brain injury.DESIGN: Randomized grouping design, controlled animal trial.SETTING: Department of Anesthesiology, the Medical School Hospital of Qingdao University.MATERIALS: Totally 150 rats of clean grade, aged 3 months, were involved and randomized into control group and ketamine-treated group, with 75 rats in each. Each group was divided into 5 subgroups separately at 6,12, 24, 48 and 72 hours after injury, with 15 rats at each time point. Main instruments and reagents:homemade beat machine, ketamine hydrochloride (Hengrui Pharmaceutical Factory, Jiangsu), rabbit anti-rat AQP-4 polyclonal antibody, SABC immunohistochemical reagent kit and TUNEL reagent kit (Boster Co.,Ltd.,Wuhan).METHODS: This trial was carried out in the Institute of Cerebrovascular Disease, Medical College of Qingdao University during March 2005 to February 2006. A weight-dropping rat model of brain injury was created with Feeney method. The rats in the ketamine-treated group were intraperitoneally administered with 50 g/L ketamine (120 mg/kg) one hour after injury, but ketamine was replaced by normal saline in the control group. In each subgroup, the water content of cerebral hemisphere was measured in 5 rats chosen randomly. The left 10 rats in each subgroup were transcardiacally perfused with ketamine, then the brain tissue was made into paraffin sections and stained by haematoxylin and eosin. Neuronal

  16. Matrix metalloproteinases and their tissue inhibitors in serum and cerebrospinal fluid of patients with moderate and severe traumatic brain injury

    Directory of Open Access Journals (Sweden)

    Kebin Zheng

    2013-01-01

    Full Text Available Objective: In this study, we investigated matrix metalloproteinases (MMPs and tissue inhibitor of metalloproteinase (TIMPs in cerebrospinal fluid (CSF and plasma of traumatic brain injury (TBI patients. Patients and Methods: A total of 30 patients with moderate and severe TBI and 15 age-matched controls were enrolled in this study. Plasma and CSF samples were collected within 24 h (as the initial value, at 72 and 120 h post injury. CSF and plasma MMP-9, MMP-2, TIMP-1 and TIMP-2 were estimated using ELISA. Different levels of these indexes were compared in the two groups and further investigated the correlation between each other. Results: There was a significant elevation in the levels of the initial MMP-9 in the CSF (P < 0.05, which lasted for 72 h post injury. TIMP-1 kept increasing within 120 h post injury and it was different compared with TIMP-1 at 24 and 72 h post injury. Plasma levels of MMP-9, MMP-2, TIMP-1 and TIMP-2 in TBI patients were also significantly different from those in controls. Furthermore the CSF MMP-9 in patients with severe TBI was higher than that in patients with moderate TBI. In addition, there was a positive relationship between the initial MMP-9 and TIMP-1 at 120 h post injury (r = 0.614, P < 0.01. Conclusion: MMPs and TIMPs are increased in both CSF and plasma of TBI patients. TIMP-1 has a positive correlation with MMP-9 and the initial MMP-9 is associated with the neurological outcomes.

  17. Connective tissue regeneration in skeletal muscle after eccentric contraction-induced injury

    DEFF Research Database (Denmark)

    Mackey, Abigail Louise; Kjaer, Michael

    2016-01-01

    the adaptation throughout this process of both the connective tissue structures surrounding the myofibres, and the fibroblasts, the cells responsible for synthesising this connective tissue. However, the few studies investigating muscle connective tissue remodelling demonstrate a strong response that appears...... when the muscle connective tissue responses are compared, although further work is required to confirm this. Pharmacological agents (growth hormone and angiotensin II type I receptor blockers) are considered in the context of accelerating the muscle connective tissue adaptation to loading. Cautioning...

  18. Effect of a hormone-releasing intrauterine system (Mirena® on aromatase and Cox-2 expression in patients with adenomyosis submitted or not, to endometrial resection

    Directory of Open Access Journals (Sweden)

    Maia R

    2012-04-01

    Full Text Available Hugo Maia Jr1,2, Clarice Haddad1, Julio Casoy1, Rebeca Maia1, Nathanael Pinheiro3, Elsimar M Coutinho11Centro de Pesquisa e Assistência em Reprodução Humana (CEPARH, 2Itaigara Memorial Day Hospital, 3IMAGEPAT, Salvador, Bahia, BrazilObjective: To investigate the effect of a levonorgestrel-releasing intrauterine system (Mirena® on aromatase and cyclooxygenase-2 (Cox-2 expression in the endometrium of patients with adenomyosis who were submitted to endometrial resection at the time of insertion, compared to a group not submitted to endometrial resection and a group of controls with adenomyosis not submitted to any previous hormonal treatment.Patients and methods: Patients with adenomyosis (n = 89 were included in this study. Twenty-two patients had been using Mirena® for 5 years but had not been submitted to endometrial resection prior to insertion of the device. Twenty-four patients were submitted to endometrial resection at the time of Mirena® insertion. The remaining 43 patients with adenomyosis had undergone no previous hormonal treatment and served as a control group. Cox-2 and aromatase expression were determined in the endometrium by immunohistochemistry.Results: Use of Mirena® for 5 years reduced aromatase expression in the endometrium; however, this reduction was significantly greater in the uteri previously submitted to endometrial resection. The reduction in Cox-2 expression was significant only in the uteri submitted to endometrial resection followed by the insertion of Mirena®.Conclusion: Endometrial resection followed by the insertion of Mirena® was associated with greater rates of amenorrhea in patients with adenomyosis, which in turn were associated with a more effective inhibition of aromatase and Cox-2 expression in the endometrium.Keywords: aromatase, Mirena®, adenomyosis, Cox-2, endometrium, levonorgestrel

  19. Gene expression arrays as a tool to unravel mechanisms of normal tissue radiation injury and prediction of response

    Institute of Scientific and Technical Information of China (English)

    Jacqueline JCM Kruse; Fiona A Stewart

    2007-01-01

    Over the past 5 years there has been a rapid increase in the use of microarray technology in the field of cancer research. The majority of studies use microarray analysis of tumor biopsies for profiling of molecular characteristics in an attempt to produce robust classifiers for prognosis. There are now several published gene sets that have been shown to predict for aggressive forms of breast cancer, where patients are most likely to benefit from adjuvant chemotherapy and tumors most likely to develop distant metastases, or be resistant to treatment. The number of publications relating to the use of microarrays for analysis of normal tissue damage, after cancer treatment or genotoxic exposure, is much more limited. A PubMed literature search was conducted using the following keywords and combination of terms: radiation, normal tissue, microarray, gene expression profiling, prediction. With respect to normal tissue radiation injury, microarrays have been used in three ways: (1) to generate gene signatures to identify sensitive and resistant populations (prognosis); (2) to identify sets of biomarker genes for estimating radiation exposure, either accidental or as a result of terrorist attack (diagnosis); (3) to identify genes and pathways involved in tissue response to injury (mechanistic). In this article we will review all (relevant) papers that covered our literature search criteria on microarray technology as it has been applied to normal tissue radiation biology and discuss how successful this has been in defining predisposition markers for radiation sensitivity or how it has helped us to unravel molecular mechanisms leading to acute and late tissue toxicity. We also discuss some of the problems and limitations in application and interpretation of such data.

  20. Human Adenomyosis Endometrium Stromal Cells Secreting More Nerve Growth Factor: Impact and Effect.

    Science.gov (United States)

    Li, Yan; Zou, Shien; Xia, Xian; Zhang, Shaofen

    2015-09-01

    Abnormal expression of nerve growth factor (NGF) was found in adenomyosis (AM). We collected AM foci from patients and eutopic endometrium from non-AM controls. Endometrium stromal cells (ESCs) were cultured. Different levels of 17β-estradiol, tumor necrosis factor (TNF), CoCl2, and H2O2 were added to the culture system separately, then the expression level of NGF in ESCs was detected. After adding different levels of NGF, the proliferation and apoptosis of ESCs and aromatase expression were detected. We found that 17β-estradiol promoted NGF production in AM ESCs but not in control ESCs; TNF promoted NGF production in both AM and control ESCs; and CoCl2 inhibited NGF production in control ESCs, but had no effect in AM ESCs. Nerve growth factor promoted the proliferation and synthesis of aromatase in AM ESCs. In conclusion, locally increased estrogen levels and inflammation may cause increased NGF production in the uterus of patients with AM. Nerve growth factor stimulated the proliferation and increased aromatase expression of ESCs from AM foci, suggesting NGF might contribute to the pathology and etiology of AM.

  1. Expression of aquaporin-1 and aquaporin-3 in lung tissue of rat model with ischemia-reperfusion injury

    Institute of Scientific and Technical Information of China (English)

    ZHAO Song; LI Xiang-nan

    2010-01-01

    @@ End-stage lung diseases are common and frequentlyoccurring diseases which are difficult for clinical treatment. In recent years, lung transplantation has become a widely accepted and effective therapeutic option for patients with the end-stage pulmonary diseases. Early pulmonary edema resulting from ischemia-reperfusion injury accounts for the major part of mortality and morbidity after lung transplantation. The water channel proteins in lung injury have been little studied, and their impact on the formation of pulmonary edema remains unclear. In this study, we established a rat lung ischemia-reperfusion model to study its impact on the expressions of water channel proteins in lung tissue and explore a new approach to lung transplantation in pulmonary edema pathogenesis.

  2. Assays of normal tissue injury and their cellular interpretation. Proceedings of the 12th L. H. Gray conference, Manchester, 2-5 September, 1985

    Energy Technology Data Exchange (ETDEWEB)

    Hendry, J.H.; Potten, C.S.; Moore, J.V.; Hume, W.J. (eds.)

    1986-01-01

    Some of the questions posed were: 1) Can target cell populations be defined in the tissue for specific types of injury 2) Are there qualitative differences in the target-cell populations for different cytotoxic agents, e.g. resistant subpopulations of cells 3) What is the cellular basis to the volume effect (i.e. greater injury per unit volume with increasing volume of tissue treated) 4) What is the contribution of intracellular repair of potentially-lethal damage to the sparing of injury in late-responding tissues 5) What are the contributions of repopulation by colony-forming cells and cells with very limited division potential to the sparing of injury in the late-responding tissues 6) How well is functional (effector) cell number correlated with overall capacity for function 7) What is the contribution of stromal (including vascular) injury in the response of the parenchyma. A total of 90 contributions considered these questions and others, in the following sections: the alimentary canal, skin, germinal tissue, vascular tissue, neuromuscular and glandular tissues, kidneys, bladder and liver, and lungs, models and strategy.

  3. Expression and significance of SOCS3 in liver tissue of rats with severe acute pancreatitis complicated by liver injury

    Directory of Open Access Journals (Sweden)

    Bin WANG

    2012-11-01

    Full Text Available Objective  To investigate the expression and mechanism of action of suppressor of cytokine signaling 3 (SOCS3 in liver tissue of rats with experimental severe acute pancreatitis (SAP concurring with liver injury. Methods  The rat model of SAP was reproduced by retrograde injection of 4% sodium taurocholate into the biliopancreatic duct. Thirty-two male SD rats were randomly assigned into 4 groups (8 each: normal control group (NC, SAP 6h, 12h, and 18h groups. The levels of serum amylase (AMY, alanine aminotransferase (ALT and aspartate aminotransferase (AST were measured dynamically. The concentrations of IL -6 and IL -18 were determined by ELISA. The localization and expression of SOCS3 protein in liver were determined by immunohistochemical staining and Western blotting. Results  Compared with NC group, the serum levels of AMY, ALT and AST increased significantly in SAP groups (P < 0.05, and there was significant difference among SAP groups. The serum concentrations of IL-6 and IL-18 increased significantly in the SAP groups than in NC group (P < 0.05, and there was significant difference among SAP groups. Compared with NC group, the concentration of SOCS3 protein increased significantly in SAP groups, and increased gradually along with the increased duration of pancreatitis (P < 0.05. A minor expression of SOCS3 protein was found in NC group. The change in SOCS3 protein concentration was consistent with the severity of liver injury as well as the serum concentrations of IL-6 and IL-18. Conclusions  The inflammatory action induced by SAP concurring with liver injury may induce the expression of SOCS3 in liver tissue, and it may increase in intensity along with the severity of liver injury and inflammatory reaction. The mechanism may be attributed to a negative feedback regulation of the inflammatory action mediated by JAK/STAT pathway.

  4. Effect of seawater immersion on periosteal function of the soft tissue with open injury in rabbit limbs

    Institute of Scientific and Technical Information of China (English)

    WANG Chang-sheng; CHENG Xun-sheng; CHEN Xiao-song; SHAN Hong-wei; LIN Zhao-fen

    2012-01-01

    Objective To investigate the effect of seawater immersion on periosteal function of the soft tissue with open injury in rabbit limbs.Methods Forty-eight New Zealand rabbits were used in the experiment.The animal model of open soft tissue injury was developed by cutting open the hindlimbs,and then the animals were randomly divided into 3 groups:the simple injury group or the control group ( group A),the injury plus 30 min seawater immersion group (group B),and the injury plus 1 h seawater immersion group (group C).Rabbits were sacrificed following surgery on 0,1,3,7 days,and histopathology and expressions of TNF-α and TGF-β1 were observed and monitored from the collected periosteum.Results (1) Inflammatory response of periosteum resulting from limb surgery plus 30 min seawater immersion was more serious than that of the animals in the control group.The longer the immersion,the more serious the response.(2) Intramembranous bone formation delayed markedly,following 1 h seawater immersion,however,for the animals in group B,delay in intramembranous bone formation was insignificant. (3) Periosteal malpighian layer cells for the animals in group B increased significantly,following injury on 1,3,7 days.Marked differences could be seen,when comparisons were made with animals of group A and group C (P < 0.01 ).Though periosteal malpighian layer cells for the animals in group A increased,when compared with those of the animals in group C,no significant differences could be noted between them ( P > 0.05 ).(4) Expressions of TNF-α were all seen in periosteal osteoprogenitor cells and osteoblasts in the animals of the 3 groups.The expression reached peak on day 1 after surgery,then,decreased gradually,with the expression level increased with the extension of seawater immersion. (5)There were expressions of TGF-β1 in periosteal osteoprogenitor cells and osteoblasts in the animals of the 3 groups and persistent expression of TGF-β1 could be observed on day 0 to day 7,and

  5. Adenomyosis and Infertility Related Factors%子宫腺肌病及其不孕相关因素

    Institute of Scientific and Technical Information of China (English)

    高艳; 宋静慧

    2014-01-01

    Adenomyosis (AM) is a common gynecological disease, in recent years, the incidence is rising. Studies on adenomyosis and infertility-related mechanisms has become a hot topic. Although there is no sufficient epidemiological data to prove that there is a genuine relationship between AM and infertility. However, with improved image and therapeutic levels in AM , indirect evidence shows AM with low fertility. There is no specific mechanism for the interpretation of adenomyosis cause of infertility, this article will combine basic pathological anatomy pathophysiology, and endometrial receptivity abnormalities, endocrine abnormalities and other aspects related to adenomyosis′s research progress, to discuss the relationship between AM and infertility factors.%子宫腺肌病(adenomyosis,AM)是妇科常见病,近年来其发病率呈上升趋势。有关AM与不孕相关机制的研究也成为近年的研究热点。虽然没有充分的流行病学数据表明AM与不孕有确切关系,但是随着AM影像水平及治疗水平的提高,间接证明AM与生殖能力低下有关。目前尚无具体诠释引起AM不孕的机制,结合AM发生的病理解剖学基础、病理生理学改变、子宫内膜容受性异常以及内分泌免疫学异常等方面的相关研究进展探讨AM与不孕的关系。

  6. Expression of GLUT4 mRNA of peripheral tissues and insulin resistance in rats with severe traumatic brain injury

    Institute of Scientific and Technical Information of China (English)

    CHEN Da-qing; ZHU Lie-lie; LI Yong-ling

    2007-01-01

    Objective: To evaluate the expression of glucose transporter-4 (GLUT4) mRNA in skeletal muscle and subcutaneous adipose tissues and investigate the mechanism of posttraumatic insulin resistance.Methods: Sixteen adult male Wistar rats were randomly divided into 2 group (n=8 in each group), i.e., severe traumatic brain injury (TBI) group due to falls from a height and normal control group. Blood glucose and serum insulin were measured at 0.5 h before trauma and 3 h, 24 h, 72 h, 7 d after trauma, respectively. And insulin sensitivity was calculated by insulin activity index (IAI) formula. Skeletal muscle and subcutaneous adipose tissue samples were collected at the same time when blood was sampled. The changes of expression of GLUT4 mRNA were observed using reverse transcription-polymerase chain reaction (RT-PCR).Results: Accompanied by the decrease of insulin sensitivity, the expression of GLUT4 mRNA was significantly decreased in adipose tissues at 24 h and 72 h after trauma (P<0.01), however, such phenomena did not appear in skeletal muscle samples.Conclusions: To some extent, the development of posttraumatic insulin resistance is related to the abnormality of transcription activity of GLUT4 gene. Adipose tissues show some difference in the transcriptional level of GLUT4 gene after trauma as compared with skeletal muscle tissues.

  7. An overview of tissue engineering approaches for management of spinal cord injuries

    Directory of Open Access Journals (Sweden)

    Samadikuchaksaraei Ali

    2007-05-01

    Full Text Available Abstract Severe spinal cord injury (SCI leads to devastating neurological deficits and disabilities, which necessitates spending a great deal of health budget for psychological and healthcare problems of these patients and their relatives. This justifies the cost of research into the new modalities for treatment of spinal cord injuries, even in developing countries. Apart from surgical management and nerve grafting, several other approaches have been adopted for management of this condition including pharmacologic and gene therapy, cell therapy, and use of different cell-free or cell-seeded bioscaffolds. In current paper, the recent developments for therapeutic delivery of stem and non-stem cells to the site of injury, and application of cell-free and cell-seeded natural and synthetic scaffolds have been reviewed.

  8. Combined anterior cruciate ligament and posterolateral reconstruction of the knee using allograft tissue in chronic knee injuries.

    Science.gov (United States)

    Fanelli, Gregory C; Fanelli, David G; Edson, Craig J; Fanelli, Matthew G

    2014-10-01

    Combined anterior cruciate ligament (ACL) and posterolateral injury of the knee can result in significant functional instability for the affected individual. Both components of the instability must be treated to maximize the probability of success for the surgical procedure. Higher failure rates of the ACL reconstruction have been reported when the posterolateral instability has been left untreated. The purpose of this article is to describe our surgical technique, and present the results of 34 chronic combined ACL posterolateral reconstructions in 34 knees using allograft tissue, and evaluating these patient outcomes with KT 1000 knee ligament arthrometer, Lysholm, Tegner, and Hospital for Special Surgery knee ligament rating scales. In addition, observations regarding patient demographics with combined ACL posterolateral instability, postoperative range of motion loss, postinjury degenerative joint disease, infection rate, return to function, and the use of radiated and nonirradiated allograft tissues will be presented.

  9. Decay-accelerating Factor Limits Hemorrhage-instigated Tissue Injury and Improves Resuscitation Clinical Parameters

    Science.gov (United States)

    2012-10-29

    hemorrhage followed by Hextend resuscitation in pigs . (A) Representative liver histologic images. (B) Hepatic injury scores. (C) Representative kidney...J Inflamm 1998;48:13. [31] Garratty G. Blood group antigens as tumor markers, parasitic /bacterial/viral receptors, and their association with

  10. 子宫腺肌症的临床超声诊断及分析%Ultrasonic Diagnosis and Clinical Analysis of Uterine Adenomyosis

    Institute of Scientific and Technical Information of China (English)

    王云田

    2015-01-01

    Objective To study the uterine adenomyosis ultrasonographic features and clinical significance. Methods 32 cases confirmed by surgery and pathology of uterine adenomyosis clinical cases were retrospectively analyzed.Results 32 patients have the uterus increases, the lesion area without envelope, muscular layer thickening, enlargement of the echo is not uniform, visible in the distribution of the smal dark areas, intima line shift, no blood flow around, see stars dot ed inside blood flow signals.Conclusion Ultrasonography in the diagnosis of uterine adenomyosis economic, accurate and fast, is the first selection of uterine adenomyosis method.%目的:探讨子宫腺肌症的声像图特点与临床意义。方法对32例经手术和病理证实为子宫腺肌症的临床病例进行回顾性分析。结果32例患者均有子宫增大,病灶区无包膜,肌层增厚,回声增粗不均匀,可见散在分布的小暗区,内膜线移位,无血流环绕,内见星点状血流信号。结论超声对子宫腺肌症的诊断经济、准确、快捷,是诊断子宫腺肌症的首选方法。

  11. Functional Regulation of the Plasma Protein Histidine-Rich Glycoprotein by Zn2+ in Settings of Tissue Injury

    Directory of Open Access Journals (Sweden)

    Kristin M. Priebatsch

    2017-03-01

    Full Text Available Divalent metal ions are essential nutrients for all living organisms and are commonly protein-bound where they perform important roles in protein structure and function. This regulatory control from metals is observed in the relatively abundant plasma protein histidine-rich glycoprotein (HRG, which displays preferential binding to the second most abundant transition element in human systems, Zinc (Zn2+. HRG has been proposed to interact with a large number of protein ligands and has been implicated in the regulation of various physiological and pathological processes including the formation of immune complexes, apoptotic/necrotic and pathogen clearance, cell adhesion, antimicrobial activity, angiogenesis, coagulation and fibrinolysis. Interestingly, these processes are often associated with sites of tissue injury or tumour growth, where the concentration and distribution of Zn2+ is known to vary. Changes in Zn2+ levels have been shown to modify HRG function by altering its affinity for certain ligands and/or providing protection against proteolytic disassembly by serine proteases. This review focuses on the molecular interplay between HRG and Zn2+, and how Zn2+ binding modifies HRG-ligand interactions to regulate function in different settings of tissue injury.

  12. Antimalarial and antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice.

    Science.gov (United States)

    Lubbad, Mahmoud Y; Al-Quraishy, Saleh; Dkhil, Mohamed A

    2015-09-01

    Malaria is still one of the most common infectious diseases and leads to various public health problems worldwide. Medicinal plants are promising sources for identifying novel agents with potential antimalarial activity. This study aimed to investigate the antimalarial and the antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice. Mice were divided into five groups. The first group served as a vehicle control; the second, third, fourth, and fifth groups were infected with 1 × 10(6) P. chabaudi-parasitized erythrocytes. Mice of the last three groups were gavaged with 100 μl of I. oblongifolia leave extract (IOLE) at a dose of 100, 200, and 300 mg IOLE/kg, respectively, once daily for 7 days. IOLE was significantly able to lower the percentage of parasitemia. The most effective dose was the 100 mg IOLE/kg, which could reduce the parasitemia from about 38 to 12 %. The infection induced spleen injury. This was evidenced by disorganization of spleen white and red pulps, appearance of hemozoin granules and parasitized erythrocytes. These changes in spleen led to the increased histological score. Also, the infection increased the spleen oxidative damage where the levels of nitrite/nitrate, malondialdehyde, and catalase were significantly altered. All these infection-induced parameters were significantly improved during IOLE treatment. In addition, the mRNA expression of inflammatory cytokines interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha were upregulated after infection with P. chabaudi, whereas IOLE significantly reduced the expression of these genes. Our results indicate that I. oblongifolia leaves extract exhibits a significant antimalarial and antioxidant effects, and protects host spleen tissue from injuries induced by P. chabaudi.

  13. Effect of Guizhifuling capsule combined with levonorgestrel releasing intrauterine system on adenomyosis

    Institute of Scientific and Technical Information of China (English)

    Dong-Tao Shi; Rui Bai

    2016-01-01

    Objective:To explore the clinical efficacy of Guizhifuling capsule combined with levonorgestrel releasing intrauterine system (LNG-IUS) in the treatment of adenomyosis (AM). Methods: A total of 80 patients with AM who were admitted in our hospital from February, 2015 to February, 2016 were included in the study and randomized into the study group and the control group. LNG-IUS was placed in patients in the two groups 7 d after menstruation. The patients in the study group were given additional Guizhifuling capsule, 3 capsules/time, 3 times/d, and the drug was withdrawn in the menstrual period. Six-month treatment was regarded as one course. PBAC was used to evaluate the menstrual blood volume before and after treatment. The chronic pain grading questionnaire method was used for dysmenorrhea degree rating. The vaginal B ultrasound 3 d after menstruation was performed to detect the uterine volume. E2, FSH, LH, and serum CA125 level before and after treatment were detected.Results: The menstrual blood volume, dysmenorrhea degree, and uterine volume after treatment in the two groups were significantly reduced when compared with before treatment (P0.05). After treatment, CA125 level in the two groups was significantly reduced when compared with before treatment (P<0.05), and the reduced degree in the study group was significantly superior to that in the control group (P<0.05).Conclusions:Guizhifuling capsule combined with LNG-IUS in the treatment of AM can significantly improve the clinical symptoms, effectively reduce CA125 level, and has no significant effect on the sex hormone levels with a satisfactory clinical efficacy; therefore, it deserves to be widely recommended in the clinic.

  14. Clinical research on the efficacy of Mirena in combined with mifepristone in the treatment of adenomyosis

    Institute of Scientific and Technical Information of China (English)

    Li-Juan Cao; Xin-Jun Shu

    2016-01-01

    Objective:To observe the clinical effect of levonorgestrel slow-release system (LNG-IUS, Mirena) in combined with mifepristone in the treatment of adenomyosis (AM). Methods:A total of 76 patients with AM who were admitted in our hospital from January, 2013 to January, 2015 were included in the study and randomized into the treatment group and the control group with 38 cases in each group. The patients in the treatment group were given Mirena in combined with mifepristone, while the patients in the control group were only placed with Mirena. The changes of symptoms and sex hormone levels before and after treatment between the two groups were compared. B ultrasound was used to examine the uterine volume and intimal thickness. Results:The comparison of menstrual cycle before and after treatment between the two groups was not statistically significant. After treatment, the menstrual duration in the two groups was significantly shortened when compared with before treatment, PBAC and VAS scores were significantly reduced. The improvement degree of dysmenorrhea after treatment in the treatment was significantly superior to that in the control group. The endometrial thickness and uterine volume after treatment in the two groups were significantly reduced when compared with before treatment, but the comparison between the two groups was not statistically significant. The serum CA125 after treatment in the treatment group was significantly lower than that in the control group. The comparison of LH, FSH, and E2 levels before and after treatment between the two groups was not statistically significant. Conclusions:Mirena in combined with mifepristone in the treatment of AM can effectively alleviate the clinical symptoms with no effect on sex hormone levels;therefore, it deserves to be widely recommended in the clinic.

  15. Human Recombinant Decay-Accelerating Factor (DAF) Increases Survival and Limits Tissue Injury After Hemorrhagic Shock

    Science.gov (United States)

    2008-12-01

    For lung injury scoring as described previously (Carraway, et al., 2003), four parameters ( alveolar fibrin edema, alveolar hemorrhage, septal...thickening and intra- alveolar inflammatory cells) were scored on each slide for 1) severity (0: absent; 1, 2 and 3 for more severe changes) and 2) extent...groups at 120 min (Table 1). Significantly reduced bicarbonate levels and base deficit were observed in the H + DAF25μg + Hextend® group at the

  16. A Standardized Rat Model of Volumetric Muscle Loss Injury for the Development of Tissue Engineering Therapies

    Science.gov (United States)

    2012-12-01

    conditions, making this assessment advantageous for the study of VML. However, hypertrophy of the EDL muscle in response to VML injury (Table 1) would...prevents com- pensatory hypertrophy of overloaded mouse extensor digito- rum longus muscle . J Appl Physiol. 1992;73:2538–2543. 34. Rosenblatt JD, Yong...D, Parry DJ. Satellite cell activity is re- quired for hypertrophy of overloaded adult rat muscle . Muscle Nerve. 1994;17:608–613. 35. Corona BT

  17. New products tissue-engineering in the treatment of spinal cord injury

    Science.gov (United States)

    Bolshakov, I. N.; Sergienko, V. I.; Kiselev, S. L.; Lagarkova, M. A.; Remigaylo, A. A.; Mihaylov, A. A.; Prokopenko, S. V.

    2015-11-01

    In the treatment of patients with complicated spinal cord injury the Russian Health spends about one million rubles for each patient in the acute and the interim period after the injury. The number of complicated spinal cord injury is different in geographical areas Russian Federation from 30 to 50 people per 1 million that is affected by the year 5600. Applied to the present surgical and pharmacological techniques provide unsatisfactory results or minimally effective treatment. Transplantation of 100 thousand neuronal mouse predecessors (24 rats) or human neuronal predecessors (18 rats) in the anatomical gap rat spinal cord, followed by analysis of neurological deficit. The neuro-matrix implantation in the rat spinal cord containing 100 thousand neuronal precursors hESC, repeatable control neuro-matrix transplantation, non-cell mass, eliminating neurological deficit for 14 weeks after transplantation about 5-9 points on the scale of the BBB. The cultivation under conditions in vitro human induced pluripotent stem cells on collagen-chitosan matrix (hIPSC) showed that neurons differentiated from induced pluripotent stem cells grown on scaffolds as compact groups and has no neurites. Cells do not penetrate into the matrix during long-term cultivation and formed near the surface of the spherical structures resembling neurospheres. At least 90% of the cells were positive for the neuronal marker tubulin b3. Further studies should be performed to examine the compatibility of neuronal cultures and matrices.

  18. In Vivo Effects of Quercetin in Association with Moderate Exercise Training in Improving Streptozotocin-Induced Aortic Tissue Injuries

    Directory of Open Access Journals (Sweden)

    Irina C. Chis

    2015-12-01

    Full Text Available Background: Diabetes mellitus (DM is a chronic endocrine-metabolic disorder associated with endothelial dysfunction. Hyperglycemia, dyslipidemia and abnormal nitric oxide-mediated vasodilatation are the major causal factors in the development of endothelial dysfunction in DM. The prevention of endothelial dysfunction may be a first target against the appearance of atherosclerosis and cardiovascular diseases. We have investigated the synergistic protective effects of quercetin administration and moderate exercise training on thoracic aorta injuries induced by diabetes. Methods: Diabetic rats that performed exercise training were subjected to a swimming training program (1 h/day, 5 days/week, 4 weeks. The diabetic rats received quercetin (30 mg/kg body weight/day for 4 weeks. At the end of the study, the thoracic aorta was isolated and divided into two parts; one part was immersed in 10% formalin for histopathological evaluations and the other was frozen for the assessment of oxidative stress markers (malondialdehyde, MDA and protein carbonyls groups, PC, the activity of antioxidant enzymes (superoxide dismutase, SOD and catalase, CAT, nitrite plus nitrate (NOx production and inducible nitric oxide synthase (iNOS protein expression. Results: Diabetic rats showed significantly increased MDA and PC levels, NOx production and iNOS expression and a reduction of SOD and CAT activity in aortic tissues. A decrease in the levels of oxidative stress markers, NOx production and iNOS expression associated with elevated activity of antioxidant enzymes in the aortic tissue were observed in quercetin-treated diabetic trained rats. Conclusions: These findings suggest that quercetin administration in association with moderate exercise training reduces vascular complications and tissue injuries induced by diabetes in rat aorta by decreasing oxidative stress and restoring NO bioavailability.

  19. Intrathecal Acetyl-L-Carnitine Protects Tissue and Improves Function after a Mild Contusive Spinal Cord Injury in Rats.

    Science.gov (United States)

    Ewan, Eric E; Hagg, Theo

    2016-02-01

    Primary and secondary ischemia after spinal cord injury (SCI) contributes to tissue and axon degeneration, which may result from decreased energy substrate availability for cellular and axonal mitochondrial adenosine triphosphate (ATP) production. Therefore, providing spinal tissue with an alternative energy substrate during ischemia may be neuroprotective after SCI. To assess this, rats received a mild contusive SCI (120 kdyn, Infinite Horizons impactor) at thoracic level 9 (T9), which causes loss of ∼ 80% of the ascending sensory dorsal column axonal projections to the gracile nucleus. Immediately afterwards, the energy substrate acetyl-L-carnitine (ALC; 1 mg/day) or phosphate-buffered saline (PBS) was infused intrathecally (sub-arachnoid) for 6 days via an L5/6 catheter attached to a subcutaneous Alzet pump. ALC treatment improved overground locomotor function (Basso-Beattie-Breshnahan [BBB] score 18 vs. 13) at 6 days, total spared epicenter (71% vs. 57%) and penumbra white matter (90% vs. 85%), ventral penumbra microvessels (108% vs. 79%), and penumbra motor neurons (42% vs. 15%) at 15 days post-SCI, compared with PBS treatment. However, the ascending sensory projections (anterogradely traced with cholera toxin B from the sciatic nerves) and dorsal column white matter and perfused blood vessels were not protected. Furthermore, grid walking, a task we have shown to be dependent on dorsal column function, was not improved. Thus, mitochondrial substrate replacement may only be efficacious in areas of lesser or temporary ischemia, such as the ventral spinal cord and injury penumbra in this study. The current data also support our previous evidence that microvessel loss is central to secondary tissue degeneration.

  20. GOOD NEWS TO THOSE SUFFERING FROM CERVICOBRACHIALGIA,LUMBAGO AND SCELALGIA AN INTRODUCTION OF DING’S THERAPY FOR THE SOFT TISSUE INJURY

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    Under the guidelines of modern medical principles,applying the theory ofmicrocirculation and receptor and by using molecular cytologic method,Dr.DingChangyi,the director of the Soft Tissue Injury and Strain Institute and its affiliatedhospital,engaged in research of the mechanism of soft tisssue injury for years,has firstcreated and developed a complete series of traditional,specific and new regimen for soft

  1. Electrical Enhancement of Healing in Combat Injuries to Hard and Soft Tissues.

    Science.gov (United States)

    1979-04-01

    correlation in a given bone or portion thereof between the amount of calcified tissue (as determined by bone ash values) and the compression or...regulating bone architecture. In: Fleisch, H. Blackwood and M. Owen, editors, Proceedings of the Third European Symposium on Calcified Tissue...1970). The effect of electric current on rat tail tendon collagen in solution. Calc. Tis. Res. 4:330. 12. Norton, L.A. and Becker, S.J. (1972). Altered

  2. Soft tissue lipoleiomyoma.

    Science.gov (United States)

    Scurry, J P; Carey, M P; Targett, C S; Dowling, J P

    1991-10-01

    A primary tumor composed of mature adipose tissue and smooth muscle occurring in the anterior abdominal wall of a 46 yr old Filipino woman is described. The tumor was not attached to the uterus, but had an appearance similar to a uterine lipoleiomyoma. It appeared as a soft, rubbery, encapsulated 11 cm ovoid mass with a uniform, white cut surface. Histologically, there were long intersecting bundles of bland smooth muscle mixed with nests of mature fat cells. The presence of differentiated smooth muscle was confirmed by fuchsinophilia, desmin positivity and electron microscopy. The patient also had 6 intramural leiomyomas, an area of adenomyosis, a subcutaneous lipoma of the subscapular region and chronic schistosomiasis of the appendix and left adnexa.

  3. Lisfranc关节损伤后局部软组织损伤对足部功能的影响%Functional evaluation of foot after Lisfranc injury with local soft tissue injury

    Institute of Scientific and Technical Information of China (English)

    孙超; 王智; 张建中

    2010-01-01

    Objective To evaluate the foot function after Lisfranc injury with local soft tissue in-jury. Methods From November 2000 to April 2006, 43 patients (at mean age of 32 years) with fresh Lisfranc injuries (45 sides) were managed with open reduction and internal fixation with screws, Kir-schner wires and AO plates. All patients had no joint surface damage and received no fusion surgery. Ac-cording to the Quenu-Kuss classification of Lisfranc injury, there were 14 patients with type-A injury, 21 with type-B and 10 with type-C. According to AO classification, there were 29 feet without severe soft tis-sue injury and 16 feet with severe soft tissue injury, of which 10 patients were combined with severe local soft tissue injury (one patient with Foot compartment syndrome). The period from injury to surgery was seven days. All patients received cast immobilization for 8-12 weeks and the foot function was evaluated by anteroposterior and lateral X-rays and American Orthopedic Foot and Ankle Society (AOFAS) score. Results All patients were followed up for 12-69 months (average 37 months), which showed that all pa-tients obtained anatomic reduction and 32 patients were satisfied with operative outcome. The average postoperative AOFAS score was 97 points for patients without severe soft tissue injury and 82 points for pa-tients with severe soft tissue injury (P<0.05). Conclusion After Lisfranc injury, the foot function is related to severity of both joint injury and local soft tissue injury.%目的 评价Lisfranc关节损伤后局部软组织损伤程度与足部功能恢复程度之间的关系.方法 自2000年11月至2006年4月,共收治新鲜Lisfranc关节损伤患者43例(45足),平均年龄32岁.所有患者关节面均无毁损,未行融合手术,均行切开复位内固定,故可排除骨性因素影响.按跖跗关节损伤Quenu-Kuss分型:A型14足,B型21足,C型10足;按AO分类局部软组织损伤程度分为:不伴严重软组织损伤组29足

  4. Skin, soft tissue and systemic bacterial infections following aquatic injuries and exposures.

    Science.gov (United States)

    Diaz, James H; Lopez, Fred A

    2015-03-01

    : Bacterial infections following aquatic injuries occur commonly in fishermen and vacationers after freshwater and saltwater exposures. Internet search engines were queried with the key words to describe the epidemiology, clinical manifestations, diagnostic and treatment strategies and outcomes of both the superficial and the deeper invasive infections caused by more common, newly emerging and unusual aquatic bacterial pathogens. Main findings included the following: (1) aquatic injuries often result in gram-negative polymicrobial infections with marine bacteria; (2) most marine bacteria are resistant to 1st- and 2nd-generation penicillins and cephalosporins; (3) nontuberculous, mycobacterial infections should be considered in late-onset, culture-negative and antibiotic-resistant marine infections; (4) superficial marine infections and pre-existing wounds exposed to seawater may result in deeply invasive infections and sepsis in immunocompromised patients. With the exception of minor marine wounds demonstrating localized cellulitis, most other marine infections and all gram-negative and mycobacterial marine infections will require therapy with antibiotic combinations.

  5. [Injury and reparative regeneration of the oral mucosal epithelium after cytostatic drugs administration (tissue, cell and molecular mechanisms)].

    Science.gov (United States)

    Bykov, V L; Leont'eva, I V

    2011-01-01

    This paper presents the systematized summary of current literature data and the authors' own findings on the regularities of human and animal surface oral mucosal epithelium (OME) injury caused by cytostatic drugs (CSD) administration, and on the ways of its regeneration after the cytostatic chemotherapy (CSCT) discontinuation. Tissue, cell and molecular mechanisms of CSCT effects on OME, are described. The direct effects of CSD included the epithelial layer attenuation with the derangement of its architecture, epitheliocyte proliferation suppression, apoptosis activation, and differentiation disturbances (involving the broad spectrum of cytological, cytochemical, ultrastructural and molecular-biological changes). In severe cases, these processes resulted in the loss of the epithelial layer integrity with the development of ulceration. Complete epithelial regeneration requires a long period after the CSCT discontinuation. Indirect effects of CSD on OME are associated with the microbial invasion and the diffusion of microbial vital activity products into the epithelium with concurrent leukopenia, immunosuppression and decreased salivary secretion.

  6. Mitochondrial dysfunction and tissue injury by alcohol, high fat, nonalcoholic substances and pathological conditions through post-translational protein modifications

    Directory of Open Access Journals (Sweden)

    Byoung-Joon Song

    2014-01-01

    Full Text Available Mitochondria are critically important in providing cellular energy ATP as well as their involvement in anti-oxidant defense, fat oxidation, intermediary metabolism and cell death processes. It is well-established that mitochondrial functions are suppressed when living cells or organisms are exposed to potentially toxic agents including alcohol, high fat diets, smoking and certain drugs or in many pathophysiological states through increased levels of oxidative/nitrative stress. Under elevated nitroxidative stress, cellular macromolecules proteins, DNA, and lipids can undergo different oxidative modifications, leading to disruption of their normal, sometimes critical, physiological functions. Recent reports also indicated that many mitochondrial proteins are modified via various post-translation modifications (PTMs and primarily inactivated. Because of the recently-emerging information, in this review, we specifically focus on the mechanisms and roles of five major PTMs (namely oxidation, nitration, phosphorylation, acetylation, and adduct formation with lipid-peroxides, reactive metabolites, or advanced glycation end products in experimental models of alcoholic and nonalcoholic fatty liver disease as well as acute hepatic injury caused by toxic compounds. We also highlight the role of the ethanol-inducible cytochrome P450-2E1 (CYP2E1 in some of these PTM changes. Finally, we discuss translational research opportunities with natural and/or synthetic anti-oxidants, which can prevent or delay the onset of mitochondrial dysfunction, fat accumulation and tissue injury.

  7. A Review of the Literature Refuting the Concept of Minor Impact Soft Tissue Injury

    Directory of Open Access Journals (Sweden)

    Christopher J Centeno

    2005-01-01

    Full Text Available BACKGROUND: Minor impact soft tissue (MIST is an insurance industry concept that seeks to identify late whiplash as a psychosocial phenomenon. However, the medical literature in this area has not been systematically reviewed since the Quebec Task Force's review in 1995.

  8. Brain tissue partial pressure of oxygen predicts the outcome of severe traumatic brain injury under mild hypothermia treatment

    Directory of Open Access Journals (Sweden)

    Sun H

    2016-08-01

    Full Text Available Hongtao Sun,1,* Maohua Zheng,2,* Yanmin Wang,1 Yunfeng Diao,1 Wanyong Zhao,1 Zhengjun Wei1 1Sixth Department of Neurosurgery, Affiliated Hospital of Logistics University of People’s Armed Police Force, Tianjin, 2Department of Neurosurgery, The First Hospital of Lanzhou University, Lanzhou, People’s Republic of China *These authors contributed equally to this work Objective: The aim of this study was to investigate the clinical significance and changes of brain tissue partial pressure of oxygen (PbtO2 in the course of mild hypothermia treatment (MHT for treating severe traumatic brain injury (sTBI. Methods: There were 68 cases with sTBI undergoing MHT. PbtO2, intracranial pressure (ICP, jugular venous oxygen saturation (SjvO2, and cerebral perfusion pressure (CPP were continuously monitored, and clinical outcomes were evaluated using the Glasgow Outcome Scale score. Results: Of 68 patients with sTBI, PbtO2, SjvO2, and CPP were obviously increased, but decreased ICP level was observed throughout the MHT. PbtO2 and ICP were negatively linearly correlated, while there was a positive linear correlation between PbtO2 and SjvO2. Monitoring CPP and SjvO2 was performed under normal circumstances, and a large proportion of patients were detected with low PbtO2. Decreased PbtO2 was also found after MHT. Conclusion: Continuous PbtO2 monitoring could be introduced to evaluate the condition of regional cerebral oxygen metabolism, thereby guiding the clinical treatment and predicting the outcome. Keywords: severe traumatic brain injury, hypothermia, brain tissue partial pressure of oxygen, therapy

  9. Effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and central secretory tissues

    Institute of Scientific and Technical Information of China (English)

    Ji Lin; Guang-Tao Yan; Xiu-Hua Hao; Lu-Huan Wang; Kai Zhang; Hui Xue

    2005-01-01

    AIM: To explore the effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and their central secretory tissues and to find out the role leptin and orexin-A play in acute inflammatory responses.METHODS: An intestinal ischemia-reperfusion (I/R)injury model of rats was established and rats were divided randomly into six groups: sham-operation group, 60 min ischemia/30 min reperfusion group (I60'R30'), I60'R90',I60'R150', I60'R240' and I60'R360', 9 rats each group.Two highly-sensitive radioimmunoassays for leptin and orexin-A were established and used to check the change of their concentrations in peripheral blood and central secretory tissues before and after intestinal I/R injury.RESULTS: Compared with the serum leptin level before injury, it decreased significantly in I60'R30' group and increased significantly in I60'R360' group; compared to sham-operation group after injury, serum leptin level increased significantly in I60'R360' group; compared to sham-operation group after injury, adipose leptin levels decreased significantly in I60'R30' and I60'R90' groups,while increased significantly in I60'R360' group. There was no significant difference between the expression levels of orexin-A before and after I/R injury.CONCLUSION: Leptin has a time-dependent response and orexin-A has a delayed response to acute inflammatory stimuli such as intestinal I/R injury and they may participate in metabolic disorders in injury as inflammatory cytokines.

  10. [Changes of menstruation patterns and adverse effects during the treatment of LNG-IUS for symptomatic adenomyosis].

    Science.gov (United States)

    Li, L; Leng, J H; Zhang, J J; Jia, S Z; Li, X Y; Shi, J H; Dai, Y; Zhang, J R; Li, T; Xu, X X; Liu, Z Z; You, S S; Chang, X Y; Lang, J H

    2016-09-25

    Objective: To investigate the changes of mestruation patterns and adverse effects during the treatment of levonorgestrel-releasing intrauterine system(LNG-IUS)for symptomatic adenomyosis in a prospective cohort study. Methods: From December, 2006 to December, 2014, patients of symptomatic adenomyosis diagnosed by transvaginal ultrasound in Peking Union Medical College Hospital were given LNG-IUS. Before and after placement of IUS, all patients' parameters were recorded, including carrying status of IUS, symptoms and scores of dysmenorrhea, menstruation scores, biochemical indicators, physical parameters, menstruation patterns and adverse effects. Risk factors for changes of menstruation patterns and adverse effects, and their impact on treatment effects were analyzed. Results: Totally 1 100 cases met inclusion criteria, with median age 36 years(range 20-44 years), median follow-up 35 months(range 1 -108 months). During follow-up changes of menstruation patterns increased significantly with amenorrhea and shortened-menstruation being the most common manifestations. On 3, 6, 12, 24, 36, 48 and 60 months after the placement of LNG-IUS, 0, 5.8%(43/744), 6.9%(47/682), 10.1%(60/595), 17.3%(87/502), 27.2%(104/383)and 29.6%(82/277)patients achieved amenorrhea respectively(P12 months after placement, abdominal pain and body weight increasing ≥5 kg/year were the most common adverse effects. Changes of menstruation patterns, total and subclassifications of adverse effects were neither dependent on patient parameters, treatment modes and treatment effects, nor could predict future LNG-IUS carrying status(all P> 0.05). After taking out of LNG-IUS, most changes of menstruation and adverse effects disappeared. Conclusions: During the treatment of LNG-IUS for symptomatic adenomyosis, changes of menstruation patterns increase gradually with amenorrhea and shortened-menstruation being the most common manifestations, while adverse effects decrease significantly. Changes of

  11. Severe Burn Injury Induces Thermogenically Functional Mitochondria in Murine White Adipose Tissue.

    Science.gov (United States)

    Porter, Craig; Herndon, David N; Bhattarai, Nisha; Ogunbileje, John O; Szczesny, Bartosz; Szabo, Csaba; Toliver-Kinsky, Tracy; Sidossis, Labros S

    2015-09-01

    Chronic cold exposure induces functionally thermogenic mitochondria in the inguinal white adipose tissue (iWAT) of mice. Whether this response occurs in pathophysiological states remains unclear. The purpose of this study was to determine the impact of severe burn trauma on iWAT mitochondrial function in mice. Male BALB/c mice (10-12 weeks) received full-thickness scald burns to ∼30% of the body surface area. Inguinal white adipose tissue was harvested from mice at 1, 4, 10, 20, and 40 days postinjury. Total and uncoupling protein 1 (UCP1)-dependent mitochondrial thermogenesis were determined in iWAT. Citrate synthase activity was determined as a proxy of mitochondrial abundance. Immunohistochemistry was performed to assess iWAT morphology and UCP1 expression. Uncoupling protein 1-dependent respiration was significantly greater at 4 and 10 days after burn compared with sham, peaking at 20 days after burn (P white adipose tissue in vivo and may offer a mechanistic explanation for the chronic hypermetabolism observed in burn victims.

  12. Carbon materials in the treatment of soft and hard tissue injuries

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    Blazewicz M.

    2001-08-01

    Full Text Available Carbon-based implant materials are of interest because they are well accepted by the biological environment. Carbon fibrous materials developed in the Department of Special Ceramics of the University of Mining and Metallurgy in Cracow were tested in in vivo studies to determine their influence on the living body. For comparative purposes, different carbon fibers were prepared and subjected to different surface modifications. Carbon materials prepared in the form of braids were implanted in subcutaneous tissue of rabbits and into skeletal muscle of rats. Carbon fabrics were examined as scaffolds in reconstruction of bone defects. The present study examined the synthesis-structure-property relationships of fibrous carbon samples with respect to the tissue response. It was shown that the tissue response depends on the form of the material form, the degree of order of the crystallites, the surface state and microstructural parameters. Carbon fibers with higher crystallinity and a better-organized graphite structure were assimilated by the body with more difficulty and small particles coming from these materials were found in the regional lymph nodes. Low-carbonized carbon fibers (small crystallite size underwent partial fragmentation and reacted with the biological environment by being gradually resorbed in the implantation site. The presence of acidic groups on the surface of the carbon fibers enhanced phagocytosis of the carbon material by macrophages. Depending on the surface state of carbon fibers different rates of bone wound healing were observed.

  13. Concentrations of Nitric Oxide in Rat Brain Tissues after Diffuse Brain Injury and Neuroprotection by the Selective Inducible Nitric Oxide Synthase Inhibitor Aminoguanidine

    Institute of Scientific and Technical Information of China (English)

    Yi-bao Wang; Shao-wu Ou; Guang-yu Li; Yun-hui Liu

    2005-01-01

    @@ To investigate the effects of nitric oxide (NO) and the selective inducible nitric oxide synthase (iNOS) inhibitor aminoguanidine (AG) on trauma, we explored the concentrations of nitric oxide in rat brain tissues at different time stamps after diffuse brain injury (DBI) with or without AG treatment.

  14. Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury

    Directory of Open Access Journals (Sweden)

    Shyue Song-Kun

    2011-02-01

    Full Text Available Abstract Background Traumatic spinal cord injury (SCI forms a disadvantageous microenvironment for tissue repair at the lesion site. To consider an appropriate time window for giving a promising therapeutic treatment for subacute and chronic SCI, global changes of proteins in the injured center at the longer survival time points after SCI remains to be elucidated. Methods Through two-dimensional electrophoresis (2DE-based proteome analysis and western blotting, we examined the differential expression of the soluble proteins isolated from the lesion center (LC at day 1 (acute and day 14 (subacute after a severe contusive injury to the thoracic spinal cord at segment 10. In situ apoptotic analysis was used to examine cell apoptosis in injured spinal cord after adenoviral gene transfer of antioxidant enzymes. In addition, administration of chondroitinase ABC (chABC was performed to analyze hindlimb locomotor recovery in rats with SCI using Basso, Beattie and Bresnahan (BBB locomotor rating scale. Results Our results showed a decline in catalase (CAT and Mn-superoxide dismutase (MnSOD found at day 14 after SCI. Accordingly, gene transfer of SOD was introduced in the injured spinal cord and found to attenuate cell apoptosis. Galectin-3, β-actin, actin regulatory protein (CAPG, and F-actin-capping protein subunit β (CAPZB at day 14 were increased when compared to that detected at day 1 after SCI or in sham-operated control. Indeed, the accumulation of β-actin+ immune cells was observed in the LC at day 14 post SCI, while most of reactive astrocytes were surrounding the lesion center. In addition, chondroitin sulfate proteoglycans (CSPG-related proteins with 40-kDa was detected in the LC at day 3-14 post SCI. Delayed treatment with chondroitinase ABC (chABC at day 3 post SCI improved the hindlimb locomotion in SCI rats. Conclusions Our findings demonstrate that the differential expression in proteins related to signal transduction, oxidoreduction

  15. Effective salvage of acute massive uterine bleeding using intrauterine balloon tamponade in a uterine adenomyosis patient on dienogest.

    Science.gov (United States)

    Nishino, Kimihiro; Hayashi, Kazumasa; Chaya, Jyunya; Kato, Noriko; Yamamuro, Osamu

    2013-03-01

    We present the case of a 37-year-old nulliparous woman in whom acute massive uterine bleeding during dienogest therapy was successfully treated using intrauterine balloon tamponade. Abnormal uterine bleeding and several cases of profuse bleeding causing severe anemia in association with dienogest therapy have been reported, but this is the first reported case involving hypovolemic shock. While successful control of postpartum hemorrhage with intrauterine balloon tamponade has been well described, applications for non-obstetric bleeding, particularly in the presence of underlying diseases, such as adenomyosis, have only rarely been reported. This procedure can be easily, promptly, and safely implemented without analgesia, anesthesia, or laparotomy; it can be used with a minimally dilated external cervical os or narrow uterine cavity; and it can preserve fertility.

  16. Partial liquid ventilation decreases tissue and serum tumor necrosis factor-α concentrations in acute lung injury model of immature piglet induced by oleic acid

    Institute of Scientific and Technical Information of China (English)

    ZHU Yao-bin; FAN Xiang-ming; LI Xiao-feng; LI Zhi-qiang; WANG Qiang; SUN Li-zhong; LIU Ying-long

    2012-01-01

    Background Pediatric patients are susceptible to lung injury.Acute lung injury in children often results in high mortality.Partial liquid ventilation (PLV) has been shown to markedly improve oxygenation and reduce histologic evidence of injury in a number of lung injury models.This study was designed to examine the hypothesis that PLV would attenuate the production of local and systemic tumor necrosis factor (TNF)-α in an immature piglet model of acute lung injury induced by oleic acid (OA).Methods Twelve Chinese immature piglets were induced acute lung injury by OA.The animals were randomly assigned to two groups of six animals,(1) conventional mechanical ventilation (MV) group and (2) PLV with 10 ml/kg FC-77 group.Results Compared with MV group,the PLV group had better cardiopulmonary variables (P <0.05).These variables included heart rate,mean blood pressure,blood pH,partial pressure of arterial oxygen (PaO2),PaO2/inspired O2 fraction (FiO2) and partial pressure of arterial carbon dioxide (PaCO2).PLV reduced TNF-α levels both in plasma and tissue compared with MV group (P <0.05).Conclusion PLV provides protective effects against TNF-a response in OA-induced acute lung injury in immature piglets.

  17. A correlation study of the expression of resistin and glycometabolism in muscle tissue after traumatic brain injury in rats

    Institute of Scientific and Technical Information of China (English)

    Jin Peng; Zhu Lielie; Zhang Jiasheng; Xie Songling; Pan Da; Wen Hao; Meng Weiyang

    2014-01-01

    Objective:To investigate the expression pattern of resistin (RSTN) in skeletal muscle tissue and its influence on glycometabolism in rats with traumatic brain injury (TBI).Methods:Seventy-eight SD rats were randomly divided into traumatic group (n=36),RSTN group (n=36) and sham operation group (n=6).Fluid percussion TBI model was developed in traumatic and RSTN groups and the latter received additional 1 mg RSTN antibody treatment for each rat.At respectively 12 h,24 h,72 h,1 w,2 w,and 4 w after operation,venous blood was collected and the right hind leg skeletal muscle tissue was sampled.We used real-time PCR to determine mRNA expression of RSTN in skeletal muscles,western blot to determine RSTN protein expression and ELISA to assess serum insulin as well as fasting blood glucose (FBG) levels.Calculation of the quantitative insulin sensitivity check index (Q value) was also conducted.The above mentioned indicators and their correction were statistically analyzed.Results:Compared with sham operation group,the RSTN expression in the skeletal muscle as well as serum insulin and FBG levels revealed significant elevation (P<0.05),and reduced Q value (P<0.05) in traumatic group.Single factor linear correlation analysis showed a significant negative correlation between RSTN expression and Q values (P<0.001) in traumatic group.Conclusion:The expression of RSTN has been greatly increased in the muscular tissue of TBI rats and it was closely related to the index of glycometabolism.RSTN may play an important role in the process of insulin resistance after TBI.

  18. Argonaute proteins in cardiac tissue contribute to the heart injury during viral myocarditis.

    Science.gov (United States)

    Sun, Shougang; Ma, Jialiang; Zhang, Quan; Wang, Qiongying; Zhou, Lei; Bai, Feng; Hu, Hao; Chang, Peng; Yu, Jing; Gao, Bingren

    2016-01-01

    MicroRNAs (miRNAs) are a group of short, noncoding, regulatory RNA molecules the dysregulation of which contributes to the pathogenesis of myocarditis. Argonaute proteins are essential components of miRNA-induced silencing complex and play important roles during miRNA biogenesis and function. However, the expression pattern of four AGO family members has not yet been detected in the coxsackievirus B3 (CVB3)-induced myocarditis tissue samples. In this study, we detected the expression of four AGOs in the CVB3-infected mouse heart tissues and found that AGO1 and AGO3 up-regulated significantly at 4 and 8h after CVB3 infection. Further in vitro research indicated that up-regulated AGO1 and AGO3 are related to the down-regulated TNFAIP3, which is a negative regulator of NF-κB pathway. Subsequently, we confirmed that TNFAIP3 is a direct target of miR-19a/b, and during CVB3 infection, the expression of miR-19a/b and miR-125a/b is not significantly changed. TNFAIP3 level is mainly reduced by up-regulated AGO1 and AGO3. This research sheds light on the relationship between overexpressed AGO proteins and CVB3-induced myocarditis, and this provides potential therapeutic target for viral myocarditis.

  19. Severe open ankle sprain (SOAS): a lesion presenting as a penetrating soft tissue injury.

    Science.gov (United States)

    Soubeyrand, Marc; Vincent-Mansour, César; Guidon, Julie; Asselineau, Alain; Ducharnes, Gildas; Molina, Véronique

    2010-01-01

    The objective of this retrospective case study was to describe the incidence and clinical features of severe open ankle sprain (SOAS), defined as a tear of the lateral or medial collateral ligaments with an associated transverse tear of the skin over the corresponding malleolus. To this end, we reviewed the medical records of patients with SOAS managed between January 2005 and January 2009, using the databases of 3 different orthopedic trauma centers. Our review revealed 9 patients with SOAS, 7 (77.77%) of which involved the lateral ligaments and 2 (22.22%) of which involved the medial ligaments. The median age was 32 (range 21 to 45) years, and the injury occurred as a result of a motor vehicle accident in 6 (66.67%) patients, and as a result of a fall from a height in 3 (33.33%) patients. Two tendons were damaged in 2 (22.22%) patients, the deep fibular nerve (deep peroneal nerve) in 2 (22.22%) patients, and the anterior tibial artery in 1 (11.11%) patient. The only abnormality on plain radiographs was pneumarthrosis, which was present in 5 (55.56%) patients. The incidence of SOAS is rare, accounting for 0.002% (9/438,000) of all trauma cases and 0.22% (9/4142) of all cases of ankle trauma. The diagnosis was confirmed by intraoperative stress-maneuvers in all 9 patients. In conclusion, SOAS should be suspected in patients who present with a traumatic skin wound over the malleolus.

  20. Protective Effects of Oyster Extract Against Hepatic Tissue Injury in Alcoholic Liver Diseases

    Institute of Scientific and Technical Information of China (English)

    ZHANG Cuiping; LI Xiaoyu; JING Xue; ZHANG Bo; ZHANG Qi; NIU Qinghui; WANG Jianjun; TIAN Zibin

    2014-01-01

    Oyster extract is an effective bioactivity component. It has abundant nutritional value and antiviral, antitumor and im-mune defense functions. The role of oyster extract in treating liver injury has been paid more attention. We use Wistar rats to make alcoholic liver disease model through injecting alcohol into rats’ stomachs. These rats were randomly divided into five groups:model group, control group, low-dose, middle-dose and high-dose experimental group with a dose of 0.12 g kg-1, 0.40 g kg-1, and 1.20 g kg-1 alcoholic. After nine weeks, serum biomarkers (ALT, AST, TG and TCHO), malondialdehyde (MDA), glutathione (GSH), C3a, C5a, IL-17, TNF-ɑ, anti-MAA-HAS IgG, CD3+, CD4+, CD8+, NK cell activation and zinc content were assessed. The results showed that the serum biomarkers(ALT, AST, TG and TCHO), MDA content, anti-MAA-HSA IgG, serum C3a, C5a IL-17 and TNF-ɑlevels of oyster extract treatment groups were significantly decreased in comparison with model group. On the contrary, GSH showed ad-verse trend. Serum CD3+, CD4+ and NK cell activation were significantly increased in middle-dose group and high-dose group compared with model group, and there was decrease of CD8+activity in high-dose group. Plasma Zn level was decreased in model group compared with that in control group. Meanwhile, Mean plasma Zn levels increased dramatically following the dose increase of a given oyster extract.

  1. Abiotic Versus Biotic Pathogens: Replicative Growth in Host Tissues Key to Discriminating Between Biotoxic Injury and Active Pathogenesis

    Science.gov (United States)

    Schuerger, Andrew C.; Ming, Douglas W.; Golden, D. C.

    2012-01-01

    Life can be defined as a self-sustaining chemical system capable of undergoing Darwinian evolution; a self-bounded, self-replicating, and self-perpetuating entity [1]. This definition should hold for terrestrial as well as extraterrestrial life-forms. Although, it is reasonable to expect that a Mars life-form would be more adaptable to Mars-like conditions than to Earth-like environments, it remains possible that negative ecological or host interactions might occur if Mars microbiota were to be inadvertently released into the terrestrial environment. A biogenic infectious agent can be defined as a self-sustaining chemical system capable of undergoing Darwinian evolution and derives its sustenance from a living cell or from the by-products of cell death. Disease can be de-fined as the detrimental alteration of one or more ordered metabolic processes in a living host caused by the continued irritation of a primary causal factor or factors; disease is a dynamic process [2]. In contrast, an injury is due to an instantaneous event; injury is not a dynamic process [2]. A causal agent of disease is defined as a pathogen, and can be either abiotic or biotic in nature. Diseases incited by biotic pathogens are the exceptions, not the norms, in terrestrial host-microbe interactions. Disease induction in a plant host can be conceptually characterized using the Disease Triangle (Fig. 1) in which disease occurs only when all host, pathogen, and environ-mental factors that contribute to the development of disease are within conducive ranges for a necessary minimum period of time. For example, plant infection and disease caused by the wheat leaf rust fungus, Puccinia recondita, occur only if virulent spores adhere to genetically susceptible host tissues for at least 4-6 hours under favorable conditions of temperature and moisture [3]. As long as one or more conditions required for disease initiation are not available, disease symptoms will not develop.

  2. The protein C pathway in tissue inflammation and injury: pathogenic role and therapeutic implications.

    Science.gov (United States)

    Danese, Silvio; Vetrano, Stefania; Zhang, Li; Poplis, Victoria A; Castellino, Francis J

    2010-02-11

    Inflammation and coagulation are closely linked interdependent processes. Under physiologic conditions, the tissue microcirculation functions in anticoagulant and anti-inflammatory fashions. However, when inflammation occurs, coagulation is also set in motion and actively participates in enhancing inflammation. Recently, novel and unexpected roles of hemostasis in the humoral and cellular components of innate immunity have been described. In particular, the protein C system, besides its well-recognized role in anticoagulation, plays a crucial role in inflammation. Indeed, the protein C system is now emerging as a novel participant in the pathogenesis of acute and chronic inflammatory diseases, such as sepsis, asthma, inflammatory bowel disease, atherosclerosis, and lung and heart inflammation, and may emerge as unexpected therapeutic targets for intervention.

  3. Uterine Artery Embolisation for Symptomatic Adenomyosis with Polyzene F-Coated Hydrogel Microspheres: Three-Year Clinical Follow-Up Using UFS–QoL Questionnaire

    Energy Technology Data Exchange (ETDEWEB)

    Nijenhuis, R. J., E-mail: nijenhuis@maastrichtuniversity.nl; Smeets, A. J., E-mail: a.smeets@elisabeth.nl; Morpurgo, M., E-mail: m.morpurgo@elisabeth.nl [St. Elisabeth Ziekenhuis, Department of Radiology (Netherlands); Boekkooi, P. F., E-mail: f.boekkooi@elisabeth.nl; Reuwer, P. J. H. M., E-mail: p.reuwer@elisabeth.nl; Smink, M., E-mail: m.smink@elisabeth.nl [St. Elisabeth Ziekenhuis, Department of Obstetrics and Gynecology (Netherlands); Rooij, W. J. van, E-mail: wjjvanrooij@gmail.com; Lohle, P. N. M., E-mail: radiol@eztilburg.nl, E-mail: paullohle@gmail.com [St. Elisabeth Ziekenhuis, Department of Radiology (Netherlands)

    2015-02-15

    PurposeThis study was designed to assess midterm outcome of uterine artery embolisation (UAE) for women with therapy-resistant adenomyosis using polyzene F-coated hydrogel microspheres.MethodsBetween September 2006 and January 2010, 29 consecutive women with adenomyosis (15 in combination with fibroids) were treated with UAE using polyzene F-coated hydrogel microspheres. Junction zone thickness was assessed with MRI at baseline and 3 months. Women filled out the uterine fibroid symptom and quality of life questionnaire at baseline, 3 months and after a mean clinical follow-up of 37 months (median 35, range 29–64 months).ResultsAt baseline, symptom severity score of 29 women was mean 67 (median 72, range 23–100). At 3 months, this score decreased to mean 22 (median 15, range 0–66) and mean 15 (median 17, range 0–34) at final follow-up. At final follow-up of mean 37 months (median 35, range 29–64 months), 22 of 29 (76 %) patients were asymptomatic. Of these 22 women, 3 underwent a second UAE at 6, 7, and 14 months. The remaining seven patients clinically improved but still had symptoms; one underwent a hysterectomy. There was no difference in outcome between women with pure adenomyosis and women with additional fibroids. The junction zone of 4 women with additional therapy was significantly thicker compared with the remaining 25 patients.ConclusionsIn women with therapy resistant adenomyosis, UAE using polyzene F-coated hydrogel microspheres resulted in 3 years preservation of the uterus in 28 of 29 (97 %) with good clinical outcome in the vast majority of patients. Initial thickness of the junction zone is related to additional therapy.

  4. The Pathophysiology of Dysmenorrhea in Adenomyosis%子宫腺肌症痛经病理生理研究进展

    Institute of Scientific and Technical Information of China (English)

    江彩霞(综述); 程忠平(审校)

    2016-01-01

    子宫腺肌症是育龄妇女的常见妇科疾病,主要的病理特征为子宫肌层内存在子宫内膜腺体与间质细胞浸润性生长。临床主要表现为月经过多与逐渐加重的进行性痛经。子宫腺肌症痛经机制十分复杂,缩宫素及其受体、炎性因子、前列腺素等可通过激发子宫平滑肌细胞痉挛收缩引起痛经;而盆腔内脏器官痛觉传导则涉及腹下交感神经纤维(第10胸椎~第1腰椎)及副交感神经纤维(第2骶椎~第4骶椎)。%Adenomyosis is a common gynecologic disorder in women in their reproductive years,which is characterized by the growth of endometrial glands and stroma into the myometrium .The main clinical manifes-tations of adenomyosis are menorrhagia and progressive dysmenorrhea.The pathogenesis of dysmenorrhea in adenomyosis is extremely complicated,oxytocin and oxytocin receptor,inflammatory factors and prostaglandin F2α( PGF2α) may be responsible for increased uterine contractility and adenomyosis associated dysmenorrhea;the pelvic viscera( internal organs) receive nerve impulses from both sympathetic ( T10-L1 ) and parasympa-thetic ( S2-4 ) nervous systems.

  5. Erythropoietin reduces apoptosis of brain tissue cells in rats after cerebral ischemia/reperfusion injury: a characteristic analysis using magnetic resonance imaging

    Directory of Open Access Journals (Sweden)

    Chun-juan Jiang

    2016-01-01

    Full Text Available Some in vitro experiments have shown that erythropoietin (EPO increases resistance to apoptosis and facilitates neuronal survival following cerebral ischemia. However, results from in vivo studies are rarely reported. Perfusion-weighted imaging (PWI and diffusion-weighted imaging (DWI have been applied successfully to distinguish acute cerebral ischemic necrosis and penumbra in living animals; therefore, we hypothesized that PWI and DWI could be used to provide imaging evidence in vivo for the conclusion that EPO could reduce apoptosis in brain areas injured by cerebral ischemia/reperfusion. To validate this hypothesis, we established a rat model of focal cerebral ischemia/reperfusion injury, and treated with intra-cerebroventricular injection of EPO (5,000 U/kg 20 minutes before injury. Brain tissue in the ischemic injury zone was sampled using MRI-guided localization. The relative area of abnormal tissue, changes in PWI and DWI in the ischemic injury zone, and the number of apoptotic cells based on TdT-mediated dUTP-biotin nick end-labeling (TUNEL were assessed. Our findings demonstrate that EPO reduces the relative area of abnormally high signal in PWI and DWI, increases cerebral blood volume, and decreases the number of apoptotic cells positive for TUNEL in the area injured by cerebral ischemia/reperfusion. The experiment provides imaging evidence in vivo for EPO treating cerebral ischemia/reperfusion injury.

  6. Erythropoietin reduces apoptosis of brain tissue cells in rats after cerebral ischemia/reperfusion injury:a characteristic analysis using magnetic resonance imaging

    Institute of Scientific and Technical Information of China (English)

    Chun-juan Jiang; Zhong-juan Wang; Yan-jun Zhao; Zhui-yang Zhang; Jing-jing Tao; Jian-yong Ma

    2016-01-01

    Somein vitro experiments have shown that erythropoietin (EPO) increases resistance to apoptosis and facilitates neuronal survival follow-ing cerebral ischemia. However, results fromin vivo studies are rarely reported. Perfusion-weighted imaging (PWI) and diffusion-weighted imaging (DWI) have been applied successfully to distinguish acute cerebral ischemic necrosis and penumbra in living animals; therefore, we hypothesized that PWI and DWI could be used to provide imaging evidencein vivo for the conclusion that EPO could reduce apoptosis in brain areas injured by cerebral ischemia/reperfusion. To validate this hypothesis, we established a rat model of focal cerebral ischemia/reperfusion injury, and treated with intra-cerebroventricular injection of EPO (5,000 U/kg) 20 minutes before injury. Brain tissue in the ischemic injury zone was sampled using MRI-guided localization. The relative area of abnormal tissue, changes in PWI and DWI in the ischemic injury zone, and the number of apoptotic cells based on TdT-mediated dUTP-biotin nick end-labeling (TUNEL) were assessed. Our ifndings demonstrate that EPO reduces the relative area of abnormally high signal in PWI and DWI, increases cerebral blood volume, and decreases the number of apoptotic cells positive for TUNEL in the area injured by cerebral ischemia/reperfusion. The experiment pro-vides imaging evidencein vivo for EPO treating cerebral ischemia/reperfusion injury.

  7. Erythropoietin reduces apoptosis of brain tissue cells in rats after cerebral ischemia/reperfusion injury: a characteristic analysis using magnetic resonance imaging.

    Science.gov (United States)

    Jiang, Chun-Juan; Wang, Zhong-Juan; Zhao, Yan-Jun; Zhang, Zhui-Yang; Tao, Jing-Jing; Ma, Jian-Yong

    2016-09-01

    Some in vitro experiments have shown that erythropoietin (EPO) increases resistance to apoptosis and facilitates neuronal survival following cerebral ischemia. However, results from in vivo studies are rarely reported. Perfusion-weighted imaging (PWI) and diffusion-weighted imaging (DWI) have been applied successfully to distinguish acute cerebral ischemic necrosis and penumbra in living animals; therefore, we hypothesized that PWI and DWI could be used to provide imaging evidence in vivo for the conclusion that EPO could reduce apoptosis in brain areas injured by cerebral ischemia/reperfusion. To validate this hypothesis, we established a rat model of focal cerebral ischemia/reperfusion injury, and treated with intra-cerebroventricular injection of EPO (5,000 U/kg) 20 minutes before injury. Brain tissue in the ischemic injury zone was sampled using MRI-guided localization. The relative area of abnormal tissue, changes in PWI and DWI in the ischemic injury zone, and the number of apoptotic cells based on TdT-mediated dUTP-biotin nick end-labeling (TUNEL) were assessed. Our findings demonstrate that EPO reduces the relative area of abnormally high signal in PWI and DWI, increases cerebral blood volume, and decreases the number of apoptotic cells positive for TUNEL in the area injured by cerebral ischemia/reperfusion. The experiment provides imaging evidence in vivo for EPO treating cerebral ischemia/reperfusion injury.

  8. Association between serum tissue inhibitor of matrix metalloproteinase-1 levels and mortality in patients with severe brain trauma injury.

    Directory of Open Access Journals (Sweden)

    Leonardo Lorente

    Full Text Available OBJECTIVE: Matrix metalloproteinases (MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs play a role in neuroinflammation after brain trauma injury (TBI. Previous studies with small sample size have reported higher circulating MMP-2 and MMP-9 levels in patients with TBI, but no association between those levels and mortality. Thus, the aim of this study was to determine whether serum TIMP-1 and MMP-9 levels are associated with mortality in patients with severe TBI. METHODS: This was a multicenter, observational and prospective study carried out in six Spanish Intensive Care Units. Patients with severe TBI defined as Glasgow Coma Scale (GCS lower than 9 were included, while those with Injury Severity Score (ISS in non-cranial aspects higher than 9 were excluded. Serum levels of TIMP-1, MMP-9 and tumor necrosis factor (TNF-alpha, and plasma levels of tissue factor (TF and plasminogen activator inhibitor (PAI-1 plasma were measured in 100 patients with severe TBI at admission. Endpoint was 30-day mortality. RESULTS: Non-surviving TBI patients (n = 27 showed higher serum TIMP-1 levels than survivor ones (n = 73. We did not find differences in MMP-9 serum levels. Logistic regression analysis showed that serum TIMP-1 levels were associated 30-day mortality (OR = 1.01; 95% CI = 1.001-1.013; P = 0.03. Survival analysis showed that patients with serum TIMP-1 higher than 220 ng/mL presented increased 30-day mortality than patients with lower levels (Chi-square = 5.50; P = 0.02. The area under the curve (AUC for TIMP-1 as predictor of 30-day mortality was 0.73 (95% CI = 0.624-0.844; P<0.001. An association between TIMP-1 levels and APACHE-II score, TNF- alpha and TF was found. CONCLUSIONS: The most relevant and new findings of our study, the largest series reporting data on TIMP-1 and MMP-9 levels in patients with severe TBI, were that serum TIMP-1 levels were associated with TBI mortality and could be used as a prognostic biomarker of mortality

  9. Repair of Cartilage injuries using in vitro engineered 3D cartilage tissue- Preliminary Results of Our Animal Studies

    Directory of Open Access Journals (Sweden)

    Arumugam S

    2011-01-01

    Full Text Available Introduction: The cartilage injuries demand novel therapeutic approaches as the success rates of the current conventional strategies for the repair of injured articular cartilages are not that encouraging. Earlier we have reported that the Thermoreversible Gelation Polymer (TGP is an ideal scaffold for human chondrocyte expansion in vitro. In this study, we report the preliminary results of the in vitro expansion, characterization and experimental in vivo transplantation of chondrocytes in a rabbit model of cartilage injury Materials & Methods: Nine rabbits were included in this study scheduled for two years, after approval by the ethics committee. In the first animal, Chondrocytes were isolated from the weight bearing area of patellar groove in the left hindlimb and cultured in TGP Scaffold and maintained at 37°C in 5% carbon dioxide incubator for 64 days without growth factors. Then the TGP-Chondrocyte construct was transplanted into an experimental defect created in the knee of the right forelimb of the same rabbit. After a period of 10 weeks, a biopsy was taken from the transplanted region and subjected to morphological analysis, characterization by histopathology (H&E stain and Immunohistochemistry (S-100 staining.Results: The chondrocytes in the 3D TGP culture had round to oval shaped morphology without any de-differentiation which is otherwise observed in Conventional 2D cultures. A macroscopic structure which resembled cartilage was appreciated in the TGP construct in vitro after 64 days which was then transplanted to the rabbit. The H&E and Immunohistochemistry studies confirmed the presence of chondrocytes in the biopsy tissue. Conclusion: Based on the results, we conclude that the TGP significantly supports the in vitro expansion of chondrocytes for a longer period and the 3D culture using TGP preserves the phenotype of the articular chondrocytes. The tissue thus grown when implanted with the TGP has engrafted well without any

  10. An athymic rat model of cutaneous radiation injury designed to study human tissue-based wound therapy

    Directory of Open Access Journals (Sweden)

    Rifkin Lucas H

    2012-05-01

    Full Text Available Abstract Purpose To describe a pilot study for a novel preclinical model used to test human tissue-based therapies in the setting of cutaneous radiation injury. Methods A protocol was designed to irradiate the skin of athymic rats while sparing the body and internal organs by utilizing a non-occlusive skin clamp along with an x-ray image guided stereotactic irradiator. Each rat was irradiated both on the right and the left flank with a circular field at a 20 cm source-to-surface distance (SSD. Single fractions of 30.4 Gy, 41.5 Gy, 52.6 Gy, 65.5 Gy, and 76.5 Gy were applied in a dose-finding trial. Eight additional wounds were created using the 41.5 Gy dose level. Each wound was photographed and the percentage of the irradiated area ulcerated at given time points was analyzed using ImageJ software. Results No systemic or lethal sequelae occurred in any animals, and all irradiated skin areas in the multi-dose trial underwent ulceration. Greater than 60% of skin within each irradiated zone underwent ulceration within ten days, with peak ulceration ranging from 62.1% to 79.8%. Peak ulceration showed a weak correlation with radiation dose (r = 0.664. Mean ulceration rate over the study period is more closely correlated to dose (r = 0.753. With the highest dose excluded due to contraction-related distortions, correlation between dose and average ulceration showed a stronger relationship (r = 0.895. Eight additional wounds created using 41.5 Gy all reached peak ulceration above 50%, with all healing significantly but incompletely by the 65-day endpoint. Conclusions We developed a functional preclinical model which is currently used to evaluate human tissue-based therapies in the setting of cutaneous radiation injury. Similar models may be widely applicable and useful the development of novel therapies which may improve radiotherapy management over a broad clinical spectrum.

  11. Role of Joshi's external stabilization system with percutaneous screw fixation in high-energy tibial condylar fractures associated with severe soft tissue injuries

    Institute of Scientific and Technical Information of China (English)

    Ashish Kumar Gupta; Rahul Sapra; Rakesh Kumar; Som Prakash Gupta; Devwart Kaushik; Sahil Gaba; Mahesh Chand Bansal

    2015-01-01

    Purpose: The treatment of high-energy tibial condylar fractures which are associated with severe sott tissue injuries remains contentious and challenging.In this study, we assessed the results of Joshi's external stabilization system (JESS) by using the principle of ligamentotaxis and percutaneous screw fixation for managing high-energy tibial condylar fractures associated with severe soft tissue injuries.Methods: Between June 2008 and June 2010, 25 consecutive patients who were 17-71 years (mean, 39.7), underwent the JESS fixation for high-energy tibial condylar fractures associated with severe soft tissue injuries.Out of 25 patients, 2 were lost during follow-up and in 1 case early removal of frame was done, leaving 22 cases for final follow-up.Among them, 11 had poor skin condition with abrasions and blisters and 2 were open injuries (Gustilo-Anderson grade Ⅰ & Ⅱ).The injury mechanisms were motor vehicle accidents (n =19), fall from a height (n =2) and assault (n =1).The fractures were classified according to Schatzker classification system.Results: There were 7 type-Ⅴ, 14 type-Ⅵ and 1 type-Ⅳ Schatzker's tibial plateau fractures.The average interval between the injury and surgery was 6.8 days (range 2-13).The average hospital stay was 13 days (range, 7-22).The average interval between the surgery and full weight bearing was 13.6 weeks (range 11-20).The average range of knee flexion was 121° (range 105°-135°).The normal extension of the knee was observed in 20 patients, and an extensor lag of 5°-8° was noted in 2 patients.The complications included superficial pin tract infections (n =4) with no knee stiffness.Conclusion: JESS with lag screw fixation combines the benefit of traction, external fixation, and limited internal fixation, at the same time as allowing the ease of access to the soft tissue for wound checks, pin care, dressing changes, measurement of compartment pressure, and the monitoring of the neurovascular status.In a nutshell, JESS

  12. Thermal injuries induce gene expression of endogenous c-fos, c-myc and bFGF in burned tissues

    Institute of Scientific and Technical Information of China (English)

    付小兵; 顾小曼; 孙同柱; 杨银辉; 孙晓庆; 盛志勇

    2003-01-01

    Objective To investigate the expression sequence and distribution characteristics of the protooncogenes c-fos, c-myc and endogenous basic fibroblast growth factor (bFGF ) genes in burned tissues, and to explore the possible effects of changes in the se genes' functions on wound healing. Methods Partial-thickness burns of 30% TBSA were established on backs of Wistar rats. Insitu hybridization and histological methods were used to detect expression of c-fos, c-myc and bFGF genes in normal and burned tissue at 3 h, 6 h, 1 d, 3 d , 7 d and 14 d postburn. Results Although expression of c-fos and c-myc genes and bFGF gene could be found in normal skin, the expression of all three were markedly induced by burn wounds and the expression models in sequence and distribution were quite different. Expre ssion of c-fos gene increased and peaked at 6 h. Signals were mainly localiz ed in both nuclei of dermal fibroblasts and monocytes. The expression of bFGF gene increased at 6 h and peaked at 1 d postburn, and was distributed in the cyt oplasm of fibroblasts. C-myc gene peaked 3 d postburn and was also distributed in the cytoplasm of fibroblasts. Conclusions These results indicated that thermal injury could induce the expression of c-fos, c-myc and bFGF at gene level, showing phasic control and regional distributi on. The phasic expression of these genes suggests that there is an interaction between protooncogenes and bFGF, which may play an important role in wound heali ng. The different expressions of c-fos and c-myc play an inducing role in reg ulating bFGF, and in turn affect wound healing.

  13. The soft tissue landmarks to avoid injury to the facial artery during filler and neurotoxin injection at the nasolabial region.

    Science.gov (United States)

    Phumyoo, Thirawass; Tansatit, Tanvaa; Rachkeaw, Natthida

    2014-09-01

    The aim of this study was to locate the course of the facial artery and to propose "the danger line" vulnerable to vascular complications following filler injection. The entire facial soft tissues were harvested from 14 Thai soft embalmed cadavers as a facial flap specimen. Measurements of the distance, the depth, and the diameter of the facial artery were done at level of the oral commissure and the nasal ala. The distance between the facial artery and the oral commissure was 15.3 ± 3.7 mm and the depth from the skin was 11.1 ± 3.1 mm. The distance between the facial artery and the nasal ala was 6.7 ± 4.4 mm and the depth was 11.6 ± 3.7 mm. The diameters of the facial artery at level of the oral commissure and the nasal ala were 2.6 ± 0.8 and 1.9 ± 0.5 mm, respectively. Maximum risk of arterial complication from dermal filler injection lateral to the oral commissure is located approximately 15 mm at the depth of 11 mm. High risk of arterial injury at the lateral nasal ala is located at 7 mm with the depth of 12 mm.

  14. Melatonin exerts a more potent effect than S-adenosyl-l-methionine against iron metabolism disturbances, oxidative stress and tissue injury induced by obstructive jaundice in rats.

    Science.gov (United States)

    Muñoz-Castañeda, Juan R; Túnez, Isaac; Herencia, Carmen; Ranchal, Isidora; González, Raúl; Ramírez, Luz M; Arjona, Alvaro; Barcos, Montserrat; Espejo, Isabel; Cruz, Adolfo; Montilla, Pedro; Padillo, Francisco J; Muntané, Jordi

    2008-07-30

    Melatonin and S-adenosyl-l-methionine (SAMe) prevent oxidative stress and tissue dysfunction in obstructive jaundice (OJ). Lipid peroxidation is exacerbated in the presence of trace amounts of iron (Fe). The study investigated the regulation by melatonin and SAMe the induction of oxidative stress, iron metabolism disturbances and tissue injury in an experimental model of OJ. Different parameters of lipid peroxidation, antioxidant status, tissue injury and Fe metabolism were determined in liver and blood. OJ induced Fe accumulation in liver, and increased transferrin (Tf) saturation and loosely bound Fe content in blood. Melatonin, and SAMe at lesser extent, enhanced protein Tf content in liver and blood, that reduced loosely bound Fe content in blood. Melatonin and SAMe did not affect ferritin (FT) and Tf mRNA expression, but reduced Tf receptor (TfR) mRNA expression in liver. In conclusion, the effect of melatonin and SAMe on Fe metabolism may be included in the beneficial properties of these agents on lipid peroxidation and tissue injury induced by OJ.

  15. Injury and regeneration of intramuscular connective tissue subjected to various regimes of distraction

    Institute of Scientific and Technical Information of China (English)

    ZHAO Li; XU Song-jie; SUN Xiao-tang

    2008-01-01

    To investigate the effect on intramuscular connective tissue and passive range of joint motion by the stress produced in limb lengthening. Methods:An animal model of limb lengthening was established in the tibia of rabbits. Distraction was initiated at a rate of 1 mm/d and 2 mm/d in two steps respectively,and both proceeded until 10%and 20% of the tibia length was achieved. Muscle samples were harvested at the time when distraction ended and at the 4th week of consolidation after the distraction. Scanning electron microscope was applied to observe the morphological changes of the perimysium. The goniometer,which we made for this study,was used to measure the passive range of joint motion.Results:The collagen fibers were partitioned in bundles, crimped and interconnected closely and orderly.In the regime of 1 mm/d distraction with 10% lengthening,no apparent changes of the collagen fiber and passive range of joint motion was demonstrated. When tibia was increased to 20%,the crimped fibers showed a tendency of being straightened while the passive range of joint motion was reduced. The findings remained the same at the 4th week of consolidation. In the regime of 2 mm/d distraction with 10% lengthening,the crimped structure of the collagen fibers in the perimysium disappeared and the fibers were almost straightened. Additionally,the interconnection of the collagen fibers became loosened and interstice was presented among the fibers. At the 4th week of consolidation,the restoration to the original crimped structure was not completed. When the lengthening ratio was increased to 20%,the collagen fibers were straightened completely. This condition remained unchanged throughout all 4 weeks. The passive range of joint motion was reduced dramatically in the regime of 2 mm/d distraction.Conclusion:The ultrastructure of perimysium and the passive range of joint motion in the regime of 1 mm/d lengthening shows the condition closest to the normal ones. The regime of 2 mm

  16. 子宫腺肌症与血清泌乳素相关性的研究%Study on the correction between the adenomyosis and serum prolactin

    Institute of Scientific and Technical Information of China (English)

    韦励; 唐莉; 曹雪辉

    2013-01-01

    目的探讨子宫腺肌症与血清泌乳素的相关性。方法选取2010~2012年于我院治疗的59例子宫腺肌症患者为观察组,另外选取59例健康女性为对照组研究对象,比较两组研究对象的血清泌乳素检测结果,探讨子宫腺肌症与血清泌乳素之间是否存在相关性。结果观察组血清泌乳素水平为(543.98+179.14)μIU/mL,明显高于对照组的(173.79±103.17)μIU/mL。另外,观察组中泌乳素阳性人群所占比例55.93%同样远高于对照组的3.39%,以上比较差异明显,有统计学意义(P<0.05)。结论子宫腺肌症患者的血清泌乳素明显异常,水平较高且阳性率高于正常人,可能与子宫腺肌症的发病机制存在关联。%Objective To study the correction between the adenomyosis and serum prolactin. Methods 59 patients with adenomyosis who were treated in our hospital from 2010 to 2012 were selected as observation group,59 healthy women were selected as control group,then serum prolactin detection results of two groups were analyzed,whether there was a correlation between adenomyosis and serum prolactin was investigated. Results The serum prolactin levels of observation group was(543.98+179.14)μIU/ml,and it was obviously higher than(173.79±103.17)μIU/ml of control group,serum prolactin positive rate of observation group was 55.93%,and it was significantly higher than 3.39%of control group,those indexes of two groups were compared,there were obvious differences(P<0.05). Conclusion The serum prolactin of patients with adenomyosis is obviously abnormal,their levels and positive rate are both higher than those of normal persons,and it may be a relationship to pathogenesis of adenomyosis.

  17. Expression of CD147 protein and MMP-9 and the significance in patients with adenomyosis%CD147蛋白和MMP-9在子宫腺肌病中的表达和意义

    Institute of Scientific and Technical Information of China (English)

    邹宁; 任云青; 李培莉; 薛丽萍

    2011-01-01

    Objective:To study the expression of CD147 and MMP-9 protein in patients with adenomyosis as well as their serun levels of soluble CD 147 in order to investigate their roles in the pathogenesis of adenomyosis. Methods: Immunohistochemistry was performed to dezect the expression of CD147 and MMP-9 protein in ectopic endometrium and eutopic endometrial tissues of 37 patients with adenomyosis(AM group) and endometrial tissues of 20 patients only with hysteromyoma( control group). The correlation between the expressions of CD147 and MMP-9 protein was evaluated. ELISA was used to detect the level of soluble CD147 proteins in the peripheral blood of AM group (37 cases with adenomyosis)and control groups(20 cases with hysteromyoma and 20 healthy cases). Results:The expression of CD147 and MMP-9 proteins in glandular and stromal cells in ectopic and eutopic endometrium of the patients with AM were significantly higher than in control group( P < 0.01 ), and the expression of CD147 and MMP-9 protein was much higher in ectopic endometrium than in eutopic endometrium of the patients with AM.The expression of CD147 protein was positively correlated with MMP-9 in patient with AM.The serum levels of soluble CD147 was much higher in AM groups than in both control group with hysteromyoma ( P > 0.05) and healthy group ( P < 0.05). Conclusion: The higher expression of CD147 protein and MMP-9 in ectopic and eutopic endometrium and serum levels of sCD147 may play an important role in the pathogenesis of AM,and CD147 protein plays its role probably through activating MMP-9 synthesis and secretion.%目的:研究CD147蛋白、MMP-9在子宫腺肌病患者组织中的表达及血清中可溶性CD147(sCD147)蛋白水平,探讨其在子宫腺肌病中的作用机制.方法:采用免疫组织化学SP法检测37例子宫腺肌病患者(子宫腺肌病组)子宫异位内膜、在位内膜及20例子宫肌瘤患者(对照组)子宫内膜组织中CD147蛋白和MMP-9的表达,并对其蛋

  18. Effects of dexmedetomidine on renal tissue after lower limb ischemia reperfusion injury in streptozotocin induced diabetic rats

    Science.gov (United States)

    Erbatur, Meral Erdal; Sezen, Şaban Cem; Bayraktar, Aslıhan Cavunt; Arslan, Mustafa; Kavutçu, Mustafa; Aydın, Muhammed Enes

    2017-01-01

    ABSTRACT Aim: The aim of this study was to investigate whether dexmedetomidine – administered before ischemia – has protective effects against lower extremity ischemia reperfusion injury that induced by clamping and subsequent declamping of infra-renal abdominal aorta in streptozotocin-induced diabetic rats. Material and Methods: After obtaining ethical committee approval, four study groups each containing six rats were created (Control (Group C), diabetes-control (Group DM-C), diabetes I/R (Group DM-I/R), and diabetes-I/R-dexmedetomidine (Group DM-I/R-D). In diabetes groups, single-dose (55 mg/kg) streptozotocin was administered intraperitoneally. Rats with a blood glucose level above 250 mg/dl at the 72nd hour were accepted as diabetic. At the end of four weeks, laparotomy was performed in all rats. Nothing else was done in Group C and DM-C. In Group DM-I/R, ischemia reperfusion was produced via two-hour periods of clamping and subsequent declamping of infra-renal abdominal aorta. In Group DM-I/R-D, 100 μg/kg dexmedetomidine was administered intraperitoneally 30 minutes before ischemia period. At the end of reperfusion, period biochemical and histopathological evaluation of renal tissue specimen were performed. Results: Thiobarbituric acid reactive substance (TBARS), Superoxide dismutase (SOD), Nitric oxide synthase (NOS), Catalase (CAT) and Glutathion S transferase (GST) levels were found significantly higher in Group DM-I/R when compared with Group C and Group DM-C. In the dexmedetomidine-treated group, TBARS, NOS, CAT, and GST levels were significantly lower than those measured in the Group D-I/R. In histopathological evaluation, glomerular vacuolization (GV), tubular dilatation (TD), vascular vacuolization and hypertrophy (VVH), tubular cell degeneration and necrosis (TCDN), tubular hyaline cylinder (THC), leucocyte infiltration (LI), and tubular cell spillage (TCS) in Group DM-I/R were significantly increased when compared with the control group

  19. Novel roles for metallothionein-I + II (MT-I + II) in defense responses, neurogenesis, and tissue restoration after traumatic brain injury: insights from global gene expression profiling in wild-type and MT-I + II knockout mice

    DEFF Research Database (Denmark)

    Penkowa, Milena; Cáceres, Mario; Borup, Rehannah

    2006-01-01

    Traumatic injury to the brain is one of the leading causes of injury-related death or disability, especially among young people. Inflammatory processes and oxidative stress likely underlie much of the damage elicited by injury, but the full repertoire of responses involved is not well known...... times consistent with the processes involved in the initial tissue injury and later regeneration of the parenchyma, as well as a prominent effect of MT-I + II deficiency. The results thoroughly confirmed the importance of the antioxidant proteins MT-I + II in the response of the brain to injury...

  20. FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration

    OpenAIRE

    Alessandra Castiglioni; Gianfranca Corna; Elena Rigamonti; Veronica Basso; Michela Vezzoli; Antonella Monno; Almada, Albert E; Anna Mondino; Wagers, Amy J.; Angelo A. Manfredi; Patrizia Rovere-Querini

    2015-01-01

    Muscle injury induces a classical inflammatory response in which cells of the innate immune system rapidly invade the tissue. Macrophages are prominently involved in this response and required for proper healing, as they are known to be important for clearing cellular debris and supporting satellite cell differentiation. Here, we sought to assess the role of the adaptive immune system in muscle regeneration after acute damage. We show that T lymphocytes are transiently recruited into the musc...

  1. MR imaging of overuse injuries in the skeletally immature gymnast: spectrum of soft-tissue and osseous lesions in the hand and wrist

    Energy Technology Data Exchange (ETDEWEB)

    Dwek, Jerry R. [Department of Radiology, Rady Children' s Hospital and Health Center, San Diego, CA (United States); Cardoso, Fabiano; Chung, Christine B. [University of California at San Diego, Department of Radiology, San Diego, CA (United States)

    2009-12-15

    In the pediatric gymnast, stress-related physeal injuries have been well described with characteristic imaging findings. However, a spectrum of overuse injuries, some rarely reported in the literature, can be encountered in the gymnast's hand and wrist. To demonstrate the MR appearance of a spectrum of overuse injuries in the skeletally immature wrist and hand of pediatric gymnasts. A total of 125 MR exams of the hand and wrist in skeletally immature children were performed at our institution during a 2-year period. Clinical histories were reviewed for gymnastics participation. MR studies of that subpopulation were reviewed and abnormalities tabulated. Of the MR studies reviewed, ten gymnasts were identified, all girls age 12-16 years (mean age 14.2 years) who presented with wrist or hand pain. Three of these children had bilateral MR exams. Abnormalities included chronic physeal injuries in three children. Two girls exhibited focal lunate osteochondral defects. Triangular fibrocartilage tears were present in three girls, one of whom had a scapholunate ligament tear. Two girls manifested metacarpal head flattening and necrosis. A variety of soft-tissue and osseous lesions can be encountered in the skeletally immature gymnast. Familiarity with these stress-related injuries is important for accurate diagnosis. (orig.)

  2. Brain tissue oxygen-based therapy and outcome after severe traumatic brain injury: a systematic literature review.

    Science.gov (United States)

    Nangunoori, Raj; Maloney-Wilensky, Eileen; Stiefel, Michael; Park, Soojin; Andrew Kofke, W; Levine, Joshua M; Yang, Wei; Le Roux, Peter D

    2012-08-01

    Observational clinical studies demonstrate that brain hypoxia is associated with poor outcome after severe traumatic brain injury (TBI). In this study, available medical literature was reviewed to examine whether brain tissue oxygen (PbtO2)-based therapy is associated with improved patient outcome after severe TBI. Clinical studies published between 1993 and 2010 that compared PbtO2-based therapy combined with intracranial and cerebral perfusion pressure (ICP/CPP)-based therapy to ICP/CPP-based therapy alone were identified from electronic databases, Index Medicus, bibliographies of pertinent articles, and expert consultation. For analysis, each selected paper had to have adequate data to determine odds ratios (ORs) and confidence intervals (CIs) of outcome described by the Glasgow outcome score (GOS). Seven studies that compared ICP/CPP and PbtO2- to ICP/CPP-based therapy were identified. There were no randomized studies and no comparison studies in children. Four studies, published in 2003, 2009, and 2010 that included 491 evaluable patients were used in the final analysis. Among patients who received PbtO2-based therapy, 121(38.8%) had unfavorable and 191 (61.2%) had a favorable outcome. Among the patients who received ICP/CPP-based therapy 104 (58.1%) had unfavorable and 75 (41.9%) had a favorable outcome. Overall PbtO2-based therapy was associated with favorable outcome (OR 2.1; 95% CI 1.4-3.1). Summary results suggest that combined ICP/CPP- and PbtO2-based therapy is associated with better outcome after severe TBI than ICP/CPP-based therapy alone. Cross-organizational practice variances cannot be controlled for in this type of review and so we cannot answer whether PbtO2-based therapy improves outcome. However, the potentially large incremental value of PbtO2-based therapy provides justification for a randomized clinical trial.

  3. Tumour necrosis factor-α inhibition with lenalidomide alleviates tissue oxidative injury and apoptosis in ob/ob obese mice.

    Science.gov (United States)

    Zhu, Xiaoling; Jiang, Shasha; Hu, Nan; Luo, Fuling; Dong, Hailong; Kang, Yu-Ming; Jones, Kyla R; Zou, Yunzeng; Xiong, Lize; Ren, Jun

    2014-07-01

    Lenalidomide (Revlimid; Selleck Chemicals, Houston, TX, USA), an analogue of thalidomide, possesses potent cytokine modulatory capacity through inhibition of cytokines such as tumour necrosis factor (TNF)-α, a cytokine pivotal for the onset and development of complications in obesity and diabetes mellitus. The present study was designed to evaluate the effect of lenalidomide on oxidative stress, protein and DNA damage in multiple organs in an ob/ob murine model of obesity. To this end, C57BL/6 lean and ob/ob obese mice were administered lenalidomide (50 mg/kg per day, p.o.) for 5 days. Oxidative stress, protein and DNA damage were assessed using the conversion of reduced glutathione (GSH) to oxidized glutathione (GSSG), carbonyl formation and Comet assay, respectively. Apoptosis was evaluated using caspase 3 activity, and levels of Bax, Bcl-2, Bip, caspase 8, caspase 9 and TNF-α were assessed using western blot analysis. Lenalidomide treatment did not affect glucose clearance in lean or ob/ob mice. Obese mice exhibited a reduced GSH/GSSG ratio in the liver, gastrocnemius skeletal muscle and small intestine, as well as enhanced protein carbonyl formation, DNA damage and caspase 3 activity in the liver, kidney, skeletal muscle and intestine; these effects were alleviated by lenalidomide, with the exception of obesity-associated DNA damage in the liver and kidney. Western blot analysis revealed elevated TNF-α, Bax, Bcl-2, Bip, caspase 8 and caspase 9 in ob/ob mice with various degrees of reversal by lenalidomide treatment. Together, these data indicate that lenalidomide protects against obesity-induced tissue injury and protein damage, possibly in association with antagonism of cytokine production and cytokine-induced apoptosis and oxidative stress.

  4. Peroxisome proliferator-activated receptor-gamma agonists suppress tissue factor overexpression in rat balloon injury model with paclitaxel infusion.

    Directory of Open Access Journals (Sweden)

    Jun-Bean Park

    Full Text Available The role and underlying mechanisms of rosiglitazone, a peroxisome proliferator-activated receptor-gamma (PPAR-γ agonist, on myocardial infarction are poorly understood. We investigated the effects of this PPAR-γ agonist on the expression of tissue factor (TF, a primary molecule for thrombosis, and elucidated its underlying mechanisms. The PPAR-γ agonist inhibited TF expression in response to TNF-α in human umbilical vein endothelial cells, human monocytic leukemia cell line, and human umbilical arterial smooth muscle cells. The overexpression of TF was mediated by increased phosphorylation of mitogen-activated protein kinase (MAPK, which was blocked by the PPAR-γ agonist. The effective MAPK differed depending on each cell type. Luciferase and ChIP assays showed that transcription factor, activator protein-1 (AP-1, was a pivotal target of the PPAR-γ agonist to lower TF transcription. Intriguingly, two main drugs for drug-eluting stent, paclitaxel or rapamycin, significantly exaggerated thrombin-induced TF expression, which was also effectively blocked by the PPAR-γ agonist in all cell types. This PPAR-γ agonist did not impair TF pathway inhibitor (TFPI in three cell types. In rat balloon injury model (Sprague-Dawley rats, n = 10/group with continuous paclitaxel infusion, the PPAR-γ agonist attenuated TF expression by 70±5% (n = 4; P<0.0001 in injured vasculature. Taken together, rosiglitazone reduced TF expression in three critical cell types involved in vascular thrombus formation via MAPK and AP-1 inhibitions. Also, this PPAR-γ agonist reversed the paclitaxel-induced aggravation of TF expression, which suggests a possibility that the benefits might outweigh its risks in a group of patients with paclitaxel-eluting stent implanted.

  5. Effects of umbilical cord tissue mesenchymal stem cells (UCX® on rat sciatic nerve regeneration after neurotmesis injuries

    Directory of Open Access Journals (Sweden)

    Gärtner A

    2013-04-01

    Full Text Available Peripheral nerves have the intrinsic capacity of self-regeneration after traumatic injury but the extent of the regeneration is often very poor. Increasing evidence demonstrates that mesenchymal stem/stromal cells (MSCs may play an important role in tissue regeneration through the secretion of soluble trophic factors that enhance and assist in repair by paracrine activation of surrounding cells. In the present study, the therapeutic value of a population of umbilical cord tissue-derived MSCs, obtained by a proprietary method (UCX®, was evaluated on end-to-end rat sciatic nerve repair. Furthermore, in order to promote both, end-to-end nerve fiber contacts and MSC cell-cell interaction, as well as reduce the flush away effect of the cells after administration, a commercially available haemostatic sealant, Floseal®, was used as vehicle. Both, functional and morphologic recoveries were evaluated along the healing period using extensor postural thrust (EPT, withdrawal reflex latency (WRL, ankle kinematics analysis, and either histological analysis or stereology, in the hyper-acute, acute and chronic phases of healing. The histological analysis of the hyper-acute and acute phase studies revealed that in the group treated with UCX ® alone the Wallerian degeneration was improved for the subsequent process of regeneration, the fiber organization was higher, and the extent of fibrosis was lower. The chronic phase experimental groups revealed that treatment with UCX® induced an increased number of regenerated fibers and thickening of the myelin sheet. Kinematics analysis showed that the ankle joint angle determined for untreated animals was significantly different from any of the treated groups at the instant of initial contact (IC. At opposite toe off (OT and heel rise (HR, differences were found between untreated animals and the groups treated with either UCX® alone or UCX® administered with Floseal®. Overall, the UCX® application presented

  6. Trans-syndesmotic fibular plating for fractures of the distal tibia and fibula with medial soft tissue injury: report of 6 cases and description of surgical technique.

    Science.gov (United States)

    Sciadini, Marcus F; Manson, Theodore T; Shah, Swapnil B

    2013-03-01

    This report presents a retrospective review of several cases of distal fractures of the tibia and fibula with significant injury to the medial soft tissues treated either primarily or in staged fashion with fixed-angle trans-syndesmotic fixation. This fixation strategy was used in an effort to minimize further surgical trauma and implant load in the zone of soft tissue injury. Ten patients were identified between September 2002 and November 2010 who presented to a level I trauma center with fractures of the distal tibia and fibula associated with open medial wounds (9 patients) or extensive closed medial degloving injury (1 patient). They were all treated with trans-syndesmotic plating of the distal fibula. Two patients were lost to follow-up after initial treatment, and an additional 2 patients had follow-up durations of only 6.5 and 3 months, respectively. This left 6 patients with an average of 23.3 months of follow-up (range: 14-36 months). Radiographs and medical records were reviewed, and clinical and radiographic results were evaluated. All 6 patients had radiographic evidence of bony healing and had resumed weight bearing. Two patients required additional bone graft surgery to encourage healing, 1 of whom also required free-flap coverage as a component of the nonunion repair. One patient resumed weight bearing earlier than instructed and experienced mild but acceptable loss of reduction. No patients developed wound infections of either the medial traumatic or lateral surgical wounds, although, as noted above, 1 of the patients with a nonunion required medial free-flap coverage as a component of the nonunion repair because of incompetent medial soft tissues. Trans-syndesmotic fixation has previously been described as providing enhanced fixation of diabetic and osteoporotic ankle fractures but has not, to our knowledge, been described for the treatment of higher energy traumatic injuries. Specifically, the valgus distal tibial fracture, frequently associated

  7. The quantitative analysis of S100 in the brain tissue and serum following diffuse brain injury in rats

    Institute of Scientific and Technical Information of China (English)

    Wang Qi; Huang Ping; Xing Bo; Tuo Ya; Zhang Yongpan; Tian Weiping; Wang Zhenyuan

    2007-01-01

    Objective To investigate the dynamics of the level of S100 in cerebrum, brainstem, and serum following the diffuse brain injury in rats and provide the experimental evidences for estimating injury time. Methods ELISA was used to determine whether S100 protein is changed after diffuse brain injury in rats. Forty rats were sacrificed at 0.5 hour, 2 hours, 4 hours, 12 hours, 24 hours, 3 d and 7 d after diffuse brain injury and normal rats as control. Results The level of S100 in cerebrum, brainstem, and serum increased, followed by a decrease, and then further increased. The level of S100 could be detected to increase at 30 minutes and reached the peak at 4 hours after DBI. The level decreased gradually to the normal at 1d and till 3 d formed the second peak. The level returned to the normal at 7d following injury again. In the postmortem injury groups, there were no significant changes compared to the control group. Conclusion The present study showed that the time-dependent expression of S100 is obvious following diffuse brain injury in rats and suggested that S100 will be a suitable marker for diffuse brain injury age determination.

  8. Nonfreezing Tissue Injuries

    Science.gov (United States)

    ... Prompt Healthier Eating Scientists Working on Solar-Powered Prosthetic Limbs Health Highlights: March 23, 2017 Fruit Juice for Kids: A Serving a Day OK ALL NEWS > Resources ... and, in rare cases, discolored areas or blisters on the affected area (usually the front of the lower leg or the top surface of the fingers). ...

  9. CHANGES OF NITRIC OXIDE LEVEL IN BRAIN TISSUES AFTER EXPERIMENTAL FOCAL INJURY%实验性颅脑损伤后脑组织NO的变化

    Institute of Scientific and Technical Information of China (English)

    李志强; 袁先厚; 袁忠惠

    2001-01-01

    Objective To explore the changes of nitric oxide (NO) level and its relationship with brain edema in acutebrain injury.Methods After acute brain injury model was produced by free dropping of rats,brain tissues were obtained andits water concentration,Ca2 + and NO were measured.Results After brain injury,the water content,NO level and Ca2 + wereall increased.The peak levels of Ca2+ and NO emerged 4 hours and 8 hours after the injury respectively,and the increasing ofCa2 + tended to last for a longer time.Tests showed that No concentration was positively correlated with the water content inbrain tissues.Conclusion NO is involved in the occurrence of brain edema after acute brain injury.%目的探讨脑损伤后急性期局部一氧化氮(NO)含量变化及其与脑水肿的关系。方法采用自由落体法制造大鼠脑损伤后,于相应时间点取出脑组织测定其含水量、NO及Ca2+浓度。结果在损伤后急性期脑组织含水量、NO及Ca2+含量均升高,其中Ca2+浓度和NO含量分别于损伤后4h、8h达高峰,且Ca2+升高趋势持续时间长。经检验,NO含量与脑组织含水量呈正相关。结论 NO在脑损伤急性期参与了脑水肿的发生过程。

  10. Live birth in a woman with recurrent implantation failure and adenomyosis following transfer of refrozen-warmed embryos

    Science.gov (United States)

    Safari, Somayyeh; Faramarzi, Azita; Khalili, Mohammad Ali

    2016-01-01

    The aim was to report a healthy live birth using re-vitrified-warmed cleavage-stage embryos derived from supernumerary warmed embryos after frozen embryo transfer (ET) in a patient with recurrent implantation failure (RIF). The case was a 39-year-old female with a history of polycystic ovarian syndrome and adenomyosis, along with RIF. After ovarian hyperstimulation, 33 cumulus-oocyte complexes were retrieved and fertilized with conventional in vitro fertilization and intracytoplasmic sperm injection. Because of the risk of ovarian hyperstimulation syndrome, 16 grade B and C embryos were vitrified. After 3 and 6 months, 3 and 4 B–C warmed embryos were transferred to the uterus, respectively. However, implantation did not take place. Ten months later, four embryos were warmed, two grade B 8-cell embryos were transferred, and two embryos were re-vitrified. One year later, the two re-vitrified cleavage-stage embryos were warmed, which resulted in a successful live birth. This finding showed that following first warming, it is feasible to refreeze supernumerary warmed embryos for subsequent ET in patients with a history of RIF. PMID:27689042

  11. Effects of diapause and cold-acclimation on the avoidance of freezing injury in fat body tissue of the rice stem borer, Chilo suppressalis Walker.

    Science.gov (United States)

    Izumi, Yohei; Sonoda, Shoji; Tsumuki, Hisaaki

    2007-07-01

    Overwintering freeze-tolerant larvae of Chilo suppressalis can survive at -25 degrees C, but non-diapausing larvae cannot. We reported earlier that to prevent intracellular freezing, which causes death in overwintering larvae of the Saigoku ecotype distributed in southwestern Japan, water leaves and glycerol enters fat body cells through water channels during freezing. However, it is still unclear how diapause and low-temperature exposure are related to the acquisition of freeze tolerance. We compared the extent of tissue damage, accumulation of glycerol, and transport of glycerol and water in fat body tissues between cold-acclimated and non-acclimated non-diapausing and diapausing larvae. The tissue from cold-acclimated diapausing larvae could survive only when frozen in Grace's insect medium with 0.25 M glycerol at -20 degrees C. The protection provided by glycerol was offset by mercuric chloride, which is a water-channel inhibitor. Fat body tissue isolated from non-acclimated diapausing larvae was injured by freezing even though glycerol was added to the medium, but the level of freezing injury was significantly lower than in non-diapausing larvae. Radiotracer assays in cold-acclimated diapausing larvae showed that during freezing, water left the cells into the medium and glycerol entered the cells from the medium at the same time. Therefore, in Saigoku ecotype larvae of the rice stem borer, both diapause and cold-acclimation are essential to accumulate glycerol and activate aquaporin for the avoidance of freezing injury.

  12. Palifermin for the protection and regeneration of epithelial tissues following injury: new findings in basic research and pre-clinical models.

    Science.gov (United States)

    Finch, Paul W; Mark Cross, Lawrence J; McAuley, Daniel F; Farrell, Catherine L

    2013-09-01

    Keratinocyte growth factor (KGF) is a paracrine-acting epithelial mitogen produced by cells of mesenchymal origin, that plays an important role in protecting and repairing epithelial tissues. Pre-clinical data initially demonstrated that a recombinant truncated KGF (palifermin) could reduce gastrointestinal injury and mortality resulting from a variety of toxic exposures. Furthermore, the use of palifermin in patients with hematological malignancies reduced the incidence and duration of severe oral mucositis experienced after intensive chemoradiotherapy. Based upon these findings, as well as the observation that KGF receptors are expressed in many, if not all, epithelial tissues, pre-clinical studies have been conducted to determine the efficacy of palifermin in protecting different epithelial tissues from toxic injury in an attempt to model various clinical situations in which it might prove to be of benefit in limiting tissue damage. In this article, we review these studies to provide the pre-clinical background for clinical trials that are described in the accompanying article and the rationale for additional clinical applications of palifermin.

  13. Mechanical Tissue Resuscitation Treatment Reduces Brain Tissue Volume and Intracerebral Hemorrhage and Increases Blood Perfusion in a Traumatic Brain Injury Model in Swine

    Science.gov (United States)

    2010-01-01

    extremities.(Covey, 2006; Geiger, 2008; Leininger , 2006) It is also used to successfully treat injuries associated with high energy trauma...case report and the role of vacuum-assisted wound closure dressing. J Orthop Trauma 19:748- 50. Leininger BE, Rasmussen TE, Smith DL, Jenkins DH

  14. The diagnostic value of low-field MRI on soft tissue Injury%低场磁共振对软组织损伤的诊断价值

    Institute of Scientific and Technical Information of China (English)

    杨建荣; 毛小明; 赵峰; 王国松; 顾利勇

    2011-01-01

    Objective:To evaluate the diagnostic value and clinical significance of Low-field MRI on tissue injury. Methods:36 patients with soft tissue injury in different parts receive MRI. The examination time was 2 h~5 d after injuring,MR regular examinations included T1WI, T2WI, FS-T2 WI (Fat-suppression, FS) , and observed the MR hehaviors of soft tissue injury. Results: Within 36 cases, there were 8 cases of musculus quadriceps injury , including 2 cases of Ⅲ degree injury, 2 cases of Ⅱ degree injury and 4 cases of Ⅰ degree injury; 6 cases of triceps muscle of calf injury, including 1 case of Ⅲ degree injury, 2 cases of Ⅱ degree injury and 3 cases of Ⅰ degree injury; 5 cases of popliteus muscle injury, including 1 case of Ⅲ degree injury, 2 cases of Ⅱ degree injury and 2 cases of Ⅰ degree injury; 7 cases of knee ti(bi)al collateral ligament injury, including 1 case of Ⅲ degree injury, 2 cases of Ⅱ degree injury and 4 cases of Ⅰ degree injury; 4 cases of posterior cruciate ligament injury, including 1 case of Ⅲ degree injury, 2 cases of Ⅱ degree injury and 1 case of Ⅰ degree injury ; 6 cases of anterior cruciate ligament injury, including 2 cases of Ⅲ degree injury, 2 cases of Ⅱ degree injury and 2 cases of Ⅰ degree injury. The behavior of MR was divided into Ⅰ ~ Ⅲ degrees, Ⅰ degree was tension injury and there were 16 cases, Ⅱ degree was partial-thickness tear and there were 12 cases, Ⅲ degree was full-thickness tear and there were 8 cases. Conclusion:MR has a higher application value on the diagnosis of muscle and tendon ligaments injury.%目的:评价低场磁共振对组织损伤的诊断价值和临床意义.方法:36例不同部位软组织损伤患者接受MR(为外伤后2h~5天)检查,包括TWI、TWI、FS-TWI(脂肪抑制序列),观察软组织损伤的MR表现.结果:36例中股四头肌Ⅲ度损伤2例,Ⅱ度损伤2例,Ⅰ度4例;小腿三头肌Ⅲ度损伤1例,Ⅱ度2例,Ⅰ度3例;胭肌Ⅲ度损伤1

  15. Humeral remodeling and soft tissue injury of the wings caused by backpack harnesses for radio transmitters in New Zealand Takahē (Porphyrio hochstetteri).

    Science.gov (United States)

    Michael, Sarah; Gartrell, Brett; Hunter, Stuart

    2013-07-01

    Backpack harnesses are commonly used to attach radio and satellite transmitters to a wide range of bird species for research and conservation management. They are an integral part of the conservation management of the New Zealand Takahē (Porphyrio hochstetteri), an endangered flightless rail. Radio transmitters mounted on backpack harnesses enable the birds to be tracked in their remaining native range of remote, mountainous Fiordland, New Zealand. We evaluated 26 Takahē retrospectively at necropsy by gross examination, radiography, and computed tomography to assess damage from the backpack harness. Ten birds that had never worn a harness had no evidence of wing injury. Of the 16 birds that had worn a harness, 10 (63%) had superficial soft tissue injury to skin or patagium or more severe injury, such as remodeling of the distal humerus at the harness cord-wing interface, or pathologic fractures. Such injuries are hypothesized to be associated with discomfort, increased risk of infection or fracture, and therefore reduced fitness. These findings have implications for all avian species deployed with backpack harnesses.

  16. The role of the plastic surgeon in dealing with soft tissue injuries: experience from the second Israel-Lebanon war, 2006.

    Science.gov (United States)

    Sharony, Zach; Eldor, Liron; Klein, Yuval; Ramon, Yitzchak; Rissin, Yaron; Berger, Yosef; Lerner, Alexander; Ullmann, Yehuda

    2009-01-01

    During the 2006 war between Israel and Lebanon, 282 Israeli soldiers were evacuated to Rambam Health Care Campus. Of these, 210 were admitted for observation or treatment, and 15 of these were admitted to the Department of Plastic and Reconstructive Surgery. Thirty-five other soldiers, hospitalized in other departments, required the care of Plastic Surgeons, either for conservative or surgical treatment. The injury profile observed was consistent with data from previous low-intensity warfare, which demonstrated that over 80% of injuries were produced by fragmentation weapons, such as artillery, mortarshells, rockets, and missiles. It differs, however, from our experience in previous wars and our expectations regarding burn wounds, both in incidence and severity, which were significantly lower as compared with the past. This article presents our management of extensive soft tissue injuries, and details 3 representative cases. It highlights the role of the Plastic Surgeon as part of the whole treatment in this type of injury and helps to predict the needs of the medical system in preparation for the future.

  17. Investigations of Tissue-Level Mechanisms of Primary Blast Injury Through Modeling, Simulation, Neuroimaging and Neuropathological Studies

    Science.gov (United States)

    2012-07-10

    than abstracts): PaperReceived TOTAL: Books Number of Manuscripts: PaperReceived TOTAL: Patents Submitted Patents Awarded Awards no additional honors... Biomechanics of traumatic brain injury. Computational methods in applied mechanics and engineering 2008;197:4692-4701. [14] Halabieh O, Wan JWL...Simulating Mechanism of Brain Injury During Closed Head Impact. Lecture Notes in Computer Science. Volume 5104/2008. Springer Berlin/Heidelberg; 2008. p 107

  18. 子宫腺肌病118例诊疗分析%Clinical analysis of 118 cases of uterine adenomyosis

    Institute of Scientific and Technical Information of China (English)

    童德梅; 陆小玲; 石杰

    2015-01-01

    目的:探讨子宫腺肌病的发病因素、临床特征及诊疗方法.方法:收治子宫腺肌病患者118例,回顾性分析其临床资料.结果:行非手术和保留子宫手术治疗共59例,宫内放置曼月乐痛经缓解率71.42%,腺肌瘤病灶切除术后,宫内放置曼月乐痛经缓解率88.24%,而单纯腺肌瘤病灶切除,未放置曼月乐痛经缓解率64.29%.本组月经紊乱或月经过多者均改善.不孕12例中,治疗后妊娠并分娩2例(16.67%).结论:在临床上可通过综合判断提高对子宫腺肌病的诊断准确率.宫内放置曼月乐是子宫腺肌病理想的辅助治疗方法.%Objective:To explore the pathogenic factors,clinical features and treatment methods of uterine adenomyosis.Methods:118 patients with adenomyosis were selected,and the clinical data were retrospectively analyzed.Results:59 cases were given non operation treatment and retain uterine operation treatment,laying up mirena in uterine cavity,dysmenorrhea relief rate was 71.42%;after adenomyoma lesions resection,laying up mirena in uterine cavity,dysmenorrhea relief rate was 88.24% ,but the pure adenomyoma lesions resection,without mirena,dysmenorrhea relief rate was 64.29% .In this group,patients with menstrual disorders or menorrhagia were improved.In 12 cases of infertility,after treatment,2 cases(16.67%) had pregnancy and childbirth. Conclusion:In clinical practice,the comprehensive judgment can improve the accuracy of diagnosis of uterine adenomyosis.Laying up mirena in uterine cavity was an ideal auxiliary treatment for uterine adenomyosis.

  19. Effect of mild hypothermia on partial pressure of oxygen in brain tissue and brain temperature in patients with severe head injury

    Institute of Scientific and Technical Information of China (English)

    张赛; 只达石; 林欣; 尚彦国; 牛玉德

    2002-01-01

    Objective: To study the changes of partial pressure of oxygen in brain tissue (PbtO2) and brain temperature (BT) in patients in acute phase of severe head injury, and to study the effect of mild hypothermia on PbtO2 and BT.   Methods: The PbtO2 and the BT of 18 patients with severe head injury were monitored, and the patients were treated with mild hypothermia within 20 hours after injury. The rectal temperature (RT) of the patients was kept on 31.5-34.9℃ for 1-7 days (57.7 hours±28.4 hours averagely), simultaneously, the indexes of PbtO2 and BT were monitored for 1-5 days (with an average of 54.8 hours±27.0 hours). According to Glasgow Outcome Scale (GOS), the prognosis of the patients was evaluated at 6 months after injury.   Results: Within 24 hours after severe head injury, the PbtO2 was significantly lower (9.6 mm Hg±6.8 mm Hg, 1 mm Hg=0.133 kPa) than the normal value (16-40 mm Hg). After treatment of mild hypothermia, the mean PbtO2 increased to 28.7 mm Hg±8.8 mm Hg during the first 24 hours, and the PbtO2 was still maintained within the range of normal value at 3 days after injury. The BT was higher than the RT in the patients in acute phase of severe head injury, and the difference between the BT and the RT significantly increased after treatment of mild hypothermia. Hyperventilation (the partial pressure of carbon dioxide in artery (PaCO2)≈25 mm Hg) decreased the high intracranial pressure (ICP) and significantly decreased the PbtO2.   Conclusions: This study demonstrates that PptO2 and BT monitoring is a safe, reliable and sensitive diagnostic method to follow cerebral oxygenation. It might become an important tool in our treatment regime for patients in the acute phase of severe head injury requiring hypothermia and hyperventilation.

  20. Dermal Fenestration With Negative Pressure Wound Therapy: A Technique for Managing Soft Tissue Injuries Associated With High-Energy Complex Foot Fractures.

    Science.gov (United States)

    Poon, Henrietta; Le Cocq, Heather; Mountain, Alistair J; Sargeant, Ian D

    2016-01-01

    Military casualties can sustain complex foot fractures from blast incidents. This frequently involves the calcaneum and is commonly associated with mid-foot fracture dislocations. The foot is at risk of both compartment syndrome and the development of fracture blisters after such injuries. The amount of energy transfer and the environment in which the injury was sustained also predispose patients to potential skin necrosis and deep infection. Decompression of the compartments is a part of accepted practice in civilian trauma to reduce the risk of complications associated with significant soft tissue swelling. The traditional methods of foot fasciotomy, however, are not without significant complications. We report a simple technique of dermal fenestration combined with the use of negative pressure wound therapy, which aims to preserve the skin integrity of the foot without resorting to formal fasciotomy.

  1. 探析针药并用治疗肩部经筋病%Study on Treatment of Acupuncture Combined with Herbs for Shoulder Soft Tissue Injuries

    Institute of Scientific and Technical Information of China (English)

    张昶; 熊会海; 王瑞红; 王军; 刘赵丽; 廖奕歆; 赵吉平

    2013-01-01

    肩部经筋病常引起肩臂疼痛及活动障碍.本病反复发作,单一治疗难以根治.文章分析了经筋的生理病理,指出治疗肩部经筋病,先以火针点刺、毫针排刺以止痛,次则依据不同证候,分别给予活血化瘀、祛风散寒除湿、补益肝肾中药内服,为针药并用治疗肩部经筋病提供理论依据.%Shoulder pain and movement disorders are common clinical symptoms of shoulder soft tissue injuries. The shoulder soft tissue injuries are usually caused,and hard to be cured. The article analyses the disease physiological of shoulder soft tissue, and suggests that fire needle combined with filiform needle can eliminate pain. Then according to different types, treatment should be to resolve blood stasis,to relieve wind,to disperse cold and eliminate wet,and to strengthen liver and invigorate the kidney. The article provides theory base for this treatment.

  2. Plastic Surgery for Repairing Facial Soft Tissue Injury%面部软组织损伤的整形美容修复

    Institute of Scientific and Technical Information of China (English)

    李咏红

    2014-01-01

    Objective:To investigate the effects and the application of cosmetic repair of patients with injury of facial soft tissue. Method:130 patients for surgery from February,2012 to December,2013 were selected,high-tech means and minimally invasive techniques was used,and plastic repair of facial soft tissue injury was adopted. Result:The repair effect is ideal with good functional recovery,without infection, hematoma and skin flap necrosis. After 1~12 months follow-up,rehabilitation effect was significant. Conclusion:The facial soft tissue injury of the situation is more complicated,and recovery effect is not ideal by using the traditional plastic method. The new technology and minimally invasive technique for facial repair is used with significant effect.%目的:探讨面部软组织损伤患者整形美容修复效果以及应用方法。方法:我所选取2012年2月至2013年12月间进行整容的130例患者,采用高科技手段与微创技术,进行面部软组织损伤的整形美容修复。结果:修复效果理想,功能恢复良好,且未出现感染、皮瓣坏死以及血肿等情况。随访1~12月后,康复效果显著。结论:面部软组织损伤的情况较为复杂,利用传统整形美容方法恢复效果不理想,我们采用新型科技手段与微创技术进行面部修复,效果显著。

  3. Kidney injury molecule-1 is up-regulated in renal epithelial cells in response to oxalate in vitro and in renal tissues in response to hyperoxaluria in vivo.

    Directory of Open Access Journals (Sweden)

    Lakshmipathi Khandrika

    Full Text Available Oxalate is a metabolic end product excreted by the kidney. Mild increases in urinary oxalate are most commonly associated with Nephrolithiasis. Chronically high levels of urinary oxalate, as seen in patients with primary hyperoxaluria, are driving factor for recurrent renal stones, and ultimately lead to renal failure, calcification of soft tissue and premature death. In previous studies others and we have demonstrated that high levels of oxalate promote injury of renal epithelial cells. However, methods to monitor oxalate induced renal injury are limited. In the present study we evaluated changes in expression of Kidney Injury Molecule-1 (KIM-1 in response to oxalate in human renal cells (HK2 cells in culture and in renal tissue and urine samples in hyperoxaluric animals which mimic in vitro and in vivo models of hyper-oxaluria. Results presented, herein demonstrate that oxalate exposure resulted in increased expression of KIM-1 m RNA as well as protein in HK2 cells. These effects were rapid and concentration dependent. Using in vivo models of hyperoxaluria we observed elevated expression of KIM-1 in renal tissues of hyperoxaluric rats as compared to normal controls. The increase in KIM-1 was both at protein and mRNA level, suggesting transcriptional activation of KIM-1 in response to oxalate exposure. Interestingly, in addition to increased KIM-1 expression, we observed increased levels of the ectodomain of KIM-1 in urine collected from hyperoxaluric rats. To the best of our knowledge our studies are the first direct demonstration of regulation of KIM-1 in response to oxalate exposure in renal epithelial cells in vitro and in vivo. Our results suggest that detection of KIM-1 over-expression and measurement of the ectodomain of KIM-1 in urine may hold promise as a marker to monitor oxalate nephrotoxicity in hyperoxaluria.

  4. Non-Immunogenic Structurally and Biologically Intact Tissue Matrix Grafts for the Immediate Repair of Ballistic-Induced Vascular and Nerve Tissue Injury in Combat Casualty Care

    Science.gov (United States)

    2005-07-01

    days after surgery. No antiplatelet or anticoagulant agents were given during or following surgery. B. Implantability Nine UVG segments from three...used in both the detergent and enzyme steps of the processing protocol as a prophylactic agent , incubation of the harvested tissue at 370C to optimize...various antibiotics covering a broad spectrum of activity and an antifungal agent . Following procurement, the tissue is shipped and stored in a

  5. One Hundred and Eighty-nine Cases of Acute Articular Soft Tissue Injury Treated by Blood-letting Puncture with Plum-blossom Needle and Cupping

    Institute of Scientific and Technical Information of China (English)

    Dai Erqing; Li Haiying; Zhao Zhankao

    2005-01-01

    @@ Acute articular soft tissue injury, a frequently encountered disease, is often clinically treated with analgesics, Chinese drugs for relaxing muscles and tendons to promote blood circulation, physiotherapy and acupuncture. These therapies can achieve certain curative effects, however, there is a longer course of treatment and some cases even have sequela particularly in such weight-carrying joints as the ankle joint and the knee joint. The therapeutic method of blood letting puncture with plum-blossom needle and cupping is adopted to treat this disease with satisfactory therapeutic effect. The results are reported as follows.

  6. Inducible nitric oxide synthase in heart tissue and nitric oxide in serum of Trypanosoma cruzi-infected rhesus monkeys: association with heart injury.

    Directory of Open Access Journals (Sweden)

    Cristiano Marcelo Espinola Carvalho

    Full Text Available BACKGROUND: The factors contributing to chronic Chagas' heart disease remain unknown. High nitric oxide (NO levels have been shown to be associated with cardiomyopathy severity in patients. Further, NO produced via inducible nitric oxide synthase (iNOS/NOS2 is proposed to play a role in Trypanosoma cruzi control. However, the participation of iNOS/NOS2 and NO in T. cruzi control and heart injury has been questioned. Here, using chronically infected rhesus monkeys and iNOS/NOS2-deficient (Nos2(-/- mice we explored the participation of iNOS/NOS2-derived NO in heart injury in T. cruzi infection. METHODOLOGY: Rhesus monkeys and C57BL/6 and Nos2(-/- mice were infected with the Colombian T. cruzi strain. Parasite DNA was detected by polymerase chain reaction, T. cruzi antigens and iNOS/NOS2(+ cells were immunohistochemically detected in heart sections and NO levels in serum were determined by Griess reagent. Heart injury was assessed by electrocardiogram (ECG, echocardiogram (ECHO, creatine kinase heart isoenzyme (CK-MB activity levels in serum and connexin 43 (Cx43 expression in the cardiac tissue. RESULTS: Chronically infected monkeys presented conduction abnormalities, cardiac inflammation and fibrosis, which resembled the spectrum of human chronic chagasic cardiomyopathy (CCC. Importantly, chronic myocarditis was associated with parasite persistence. Moreover, Cx43 loss and increased CK-MB activity levels were primarily correlated with iNOS/NOS2(+ cells infiltrating the cardiac tissue and NO levels in serum. Studies in Nos2(-/- mice reinforced that the iNOS/NOS2-NO pathway plays a pivotal role in T. cruzi-elicited cardiomyocyte injury and in conduction abnormalities that were associated with Cx43 loss in the cardiac tissue. CONCLUSION: T. cruzi-infected rhesus monkeys reproduce features of CCC. Moreover, our data support that in T. cruzi infection persistent parasite-triggered iNOS/NOS2 in the cardiac tissue and NO overproduction might contribute

  7. Abnormal Activation of RhoA/ROCK-I Signaling in Junctional Zone Smooth Muscle Cells of Patients With Adenomyosis.

    Science.gov (United States)

    Wang, S; Duan, H; Zhang, Y; Sun, F Q

    2016-03-01

    Adenomyosis (ADS) is a common estrogen-dependent gynecological disease with unknown etiology. The RhoA/Rho-kinase (ROCK) signaling pathway is involved in various cellular functions, including migration, proliferation, and smooth muscle contraction. Here we examined the potential role of this pathway in junctional zone (JZ) contraction in women with and without ADS. We demonstrated that in the normal JZ, RhoA and ROCK-I messenger RNA (mRNA) and protein expression was significantly higher in the proliferative phase of the menstrual cycle than in the secretory phase. Expression of RhoA and ROCK-I in the JZ from women with ADS was significantly higher than in the control women and showed no significant differences across the menstrual cycle. Treatment of JZ smooth muscle cells (JZSMCs) with estrogen at 0, 1, 10, or 100 nmol/L for 24 hours resulted in increased expression of RhoA, ROCK-I, and myosin light-chain (MLC) phosphorylation (p-MLC) in a dose-dependent manner. In parallel to its effects on p-MLC, estrogen-mediated, dose-dependent contraction responses in JZSMCs. Estrogen-mediated contraction in the ADS group was significantly higher than in the controls and also showed no significant differences across the menstrual cycle. These effects were suppressed in the presence of ICI 182780 or Y27632, supporting an estrogen receptor-dependent and RhoA activation-dependent mechanism. Our results indicate that the level of RhoA and ROCK-I increases in patients with ADS and the cyclic change is lost. Estrogen may affect uterine JZ contraction of ADS by enhancing RhoA/ ROCK-I signaling.

  8. 干细胞参与运动损伤的组织修复%Stem cells involved in the tissue repair of sports injury

    Institute of Scientific and Technical Information of China (English)

    杨波; 梁静群; 刘涛; 王王; 张翔

    2011-01-01

    BACKGROUND: Previous studies demonstrated that mesenchymal stem cells (MSCs) have the potential of multi-directional differentiation, and applied to many field. Stem cells therapy will be a revolutionary progress for tissue injury repairing.OBJECTIVE: To summarize the situation and new progress of soft tissue injury with stem cells therapy at home and abroad.METHODS: The articles related to stem cells involved in the treatment of soft tissue injury in CNKI database and Elsevier database from January 2000 to September 2010 were retrieved by computer with the key words of "stem cells, treatment, soft tissues injury" in Chinese and in English. The content of articles related to stem cells therapy, and recently published or published in authoritative magazines in the same field were selected, and 36 documents of them were involved for summarization.RESULTS AND CONCLUSION : The competitive state of athletes was seriously affected by tendon, skeletal injury in sports, and it is difficult to radical cure, often recurrent attacks. The research of stem cells technology has a great significance in the treatment of injury of athletes. At present, MSCs as a basis of cells therapy is becoming a hot spot and most forward in the field of regenerative medicine research. Therefore, MSCs as the most important source of adult stem cells has a vital application prospect.%背景:既往多项研究已证实间充质干细胞具备多向分化潜力,并应用到多个领域,同样干细胞疗法对解决组织损伤修复将是一种革命性进步.目的:对国内外应用干细胞治疗软组织损伤的现状及新进展作一综述.方法:应用计算机检索CNKI和Elsevier数据库中2000-01/2010-09关于干细胞参与软组织损伤治疗的文章,在标题和摘要中以"干细胞,治疗,损伤"或"stem cells,treatment,Soft tissues injure"为检索词进行检索.选择文章内容与干细胞治疗有关者,同一领域文献则选择近期发表或发表在权威杂志文章.

  9. The quantitative analysis of S100 in the brain tissue and serum following diffusebrain injury in rats

    Institute of Scientific and Technical Information of China (English)

    王琪; 黄平; 邢博; 托娅; 张勇攀; 田卫平; 王振原

    2007-01-01

    Wound examination is one of the most i mpor-tant aspects inthe forensic practice,and forensic pa-thologists are often required to esti mate woundsage.The diffuse brain injury(DBI)exists in theforensic practices widely.At present,various kindsof biological substances such as c-fos,β-App,FN,and appolipoprotein Eare knownto be closely relat-ed to braininjury,but no effective methods can beused to differentiate the antemortem diffuse braininjuries from the post mortem injuries and exactlyesti mate the ti me of ...

  10. Repair of full-thickness tendon injury using connective tissue progenitors efficiently derived from human embryonic stem cells and fetal tissues.

    Science.gov (United States)

    Cohen, Shahar; Leshansky, Lucy; Zussman, Eyal; Burman, Michael; Srouji, Samer; Livne, Erella; Abramov, Natalie; Itskovitz-Eldor, Joseph

    2010-10-01

    The use of stem cells for tissue engineering (TE) encourages scientists to design new platforms in the field of regenerative and reconstructive medicine. Human embryonic stem cells (hESC) have been proposed to be an important cell source for cell-based TE applications as well as an exciting tool for investigating the fundamentals of human development. Here, we describe the efficient derivation of connective tissue progenitors (CTPs) from hESC lines and fetal tissues. The CTPs were significantly expanded and induced to generate tendon tissues in vitro, with ultrastructural characteristics and biomechanical properties typical of mature tendons. We describe a simple method for engineering tendon grafts that can successfully repair injured Achilles tendons and restore the ankle joint extension movement in mice. We also show the CTP's ability to differentiate into bone, cartilage, and fat both in vitro and in vivo. This study offers evidence for the possibility of using stem cell-derived engineered grafts to replace missing tissues, and sets a basic platform for future cell-based TE applications in the fields of orthopedics and reconstructive surgery.

  11. Comparison of an Additional Transdermal Fentanyl Patch Compared to Intravenous NSAID and Opioid Analgesics within 24 Hours of an Uterine Artery Embolization for Myoma and Adenomyosis

    Energy Technology Data Exchange (ETDEWEB)

    Song, Suk Yun; Kang, Byung Chul; Rho, Kyung Min [Dept. of Radiology, Mokdong Hospital, Ewha Womans University School of Medicine, Seoul (Korea, Republic of)

    2011-05-15

    To evaluate the effectiveness of an additional transdermal fentanyl patch compared to intravenous analgesics in pain control during the 24-hour period following uterine artery embolization (UAE) for myoma and adenomyosis. Between September 2009 and August 2010, 42 patients underwent UAE for myoma or adenomyosis. Of these, 21 received an intravenous opioid (pethidine) and a nonsteroidal anti-inflammatory drug (group A), and 21 received an additional transdermal fentanyl patch (group B). Pain perception levels were established verbally on a 0-10 scale during the 24-hour period following UAE. Differences in pain trends, mean dose of intravenous pethidine, and adverse effects were compared between the two groups. Pain perception was most severe at 6 hours after UAE and the mean pain level of group B at that time was 6.3 {+-} 0.7, which was significantly lower than that of group A, 8.2 {+-} 0.7 (p<0.05). The mean dose of intravenous pethidine was 114.3 {+-} 59.5 mg in group A and 90.5 {+-} 49.0 mg in group B, while the incidence of nausea was 67% in group A and 77% in group B. In both cases, the differences were not significantly different (p>0.05), and no evidence of respiratory distress was demonstrated. The addition of a transdermal fentanyl patch to intravenous analgesics is effective in reducing post-embolization pain during the 24-hour period after UAE.

  12. Deep tissue injury in development of pressure ulcers: a decrease of inflammasome activation and changes in human skin morphology in response to aging and mechanical load.

    Directory of Open Access Journals (Sweden)

    Olivera Stojadinovic

    Full Text Available Molecular mechanisms leading to pressure ulcer development are scarce in spite of high mortality of patients. Development of pressure ulcers that is initially observed as deep tissue injury is multifactorial. We postulate that biomechanical forces and inflammasome activation, together with ischemia and aging, may play a role in pressure ulcer development. To test this we used a newly-developed bio-mechanical model in which ischemic young and aged human skin was subjected to a constant physiological compressive stress (load of 300 kPa (determined by pressure plate analyses of a person in a reclining position for 0.5-4 hours. Collagen orientation was assessed using polarized light, whereas inflammasome proteins were quantified by immunoblotting. Loaded skin showed marked changes in morphology and NLRP3 inflammasome protein expression. Sub-epidermal separations and altered orientation of collagen fibers were observed in aged skin at earlier time points. Aged skin showed significant decreases in the levels of NLRP3 inflammasome proteins. Loading did not alter NLRP3 inflammasome proteins expression in aged skin, whereas it significantly increased their levels in young skin. We conclude that aging contributes to rapid morphological changes and decrease in inflammasome proteins in response to tissue damage, suggesting that a decline in the innate inflammatory response in elderly skin could contribute to pressure ulcer pathogenesis. Observed morphological changes suggest that tissue damage upon loading may not be entirely preventable. Furthermore, newly developed model described here may be very useful in understanding the mechanisms of deep tissue injury that may lead towards development of pressure ulcers.

  13. Soft tissue injury related to choice of entry point in antegrade femoral nailing : piriform fossa or greater trochanter tip

    NARCIS (Netherlands)

    Moein, CMA; Verhofstad, MHJ; Bleys, RLAW; van der Werken, C

    2005-01-01

    Intramedullary nailing through the piriform fossa results in some cases in toss of abduction strength and persistent pain. Nail insertion at the tip of the greater trochanter may be favourable. The aim of this study was to assess (possible) iatrogenic injury to the abductor and external rotator musc

  14. Soft tissue injury related to choice of entry point in antegrade femoral nailing: piriform fossa or greater trochanter tip

    NARCIS (Netherlands)

    Moein, C.M.A.; Verhofstad, M.H.J.; Bleys, R.L.A.W.; van der Werken, C.

    2005-01-01

    Intramedullary nailing through the piriform fossa results in some cases in toss of abduction strength and persistent pain. Nail insertion at the tip of the greater trochanter may be favourable. The aim of this study was to assess (possible) iatrogenic injury to the abductor and external rotator musc

  15. Targeted complement inhibition by C3d recognition ameliorates tissue injury without apparent increase in susceptibility to infection.

    Science.gov (United States)

    Atkinson, Carl; Song, Hongbin; Lu, Bo; Qiao, Fei; Burns, Tara A; Holers, V Michael; Tsokos, George C; Tomlinson, Stephen

    2005-09-01

    Previous studies indicate a pivotal role for complement in mediating both local and remote injury following ischemia and reperfusion of the intestine. Here, we report on the use of a mouse model of intestinal ischemia/reperfusion injury to investigate the strategy of targeting complement inhibition to sites of complement activation by linking an iC3b/C3dg-binding fragment of mouse complement receptor 2 (CR2) to a mouse complement-inhibitory protein, Crry. We show that the novel CR2-Crry fusion protein targets sites of local and remote (lung) complement activation following intestinal ischemia and reperfusion injury and that CR2-Crry requires a 10-fold lower dose than its systemic counterpart, Crry-Ig, to provide equivalent protection from both local and remote injury. CR2-Crry has a significantly shorter serum half-life than Crry-Ig and, unlike Crry-Ig, had no significant effect on serum complement activity at minimum effective therapeutic doses. Furthermore, the minimum effective dose of Crry-Ig significantly enhanced susceptibility to infection in a mouse model of acute septic peritonitis, whereas the effect of CR2-Crry on susceptibility to infection was indistinguishable from that of PBS control. Thus, compared with systemic inhibition, CR2-mediated targeting of a complement inhibitor of activation improved bioavailability, significantly enhanced efficacy, and maintained host resistance to infection.

  16. Contents of myelin-basic protein and S-100 in serum and brain tissue of neonatal rats with intrauterine infection-caused brain injury

    Institute of Scientific and Technical Information of China (English)

    Xiaojie Li; Hongying Li; Zhihai Lu

    2006-01-01

    BACKGROUND: The change of the content of myelin basic protein (MBP) in serum and brain tissue is the bio chemical diadynamic index of amyelination. S-100 is a specific and sensitive marker of central nervous system (CNS) injury. Whether or not the content of S-100 and MBP in blood and brain tissue can be used as the quan titative index for early diagnosing the intrauterine infection-caused brain injury still needs investigation. OBJECTIVE: To observe whether or not MBP and S-100 detection can be used as the biochemical indexes for early diagnosing the intrauterine infection-caused brain injury. DESIGN: Randomized controlled animal experiment. SETTING: Laboratory of Pediatric Neuro-rehabilitation, Medical College of Rehabilitation, Jiamusi University. MATERIALS: Sixty female and thirty male common Wistar rats, weighing from 180 to 240 g, were provided by the Experimental Animal Center of Jiamusi University. Reagent: Lipopolysaccharide(LPS, serological type 055: B5, SIGMA Company of USA); MBP enzyme linked immunosobent assay (ELISA) immunoreagent kit (Preclinicai Recombination DNA Laboratory, Chengdu Huaxi Medical Center, Sichuan Province); S-100 ELISA immunoreagent kit ( Department of Physiology, the Fourth Military Medical University of Chinese PLA) and bovine serum albumin(Haitaike Biotechnology Co.,Ltd.).METHODS: This experiment was carried out in the Laboratory of Pediatric Neuro-Rehabilitation, Experimental Animal Center, Department of Pathology and Central Laboratory of Jiamusi University from July 2005 to March 2006. ① Preparation of models and grouping: The female and male rats were placed in one cage at 2: 1 at 17:00 o'clock. Vaginal smear was checked at 8:00 on the next morning. Sperm was found and 0 day of pregnancy was recorded. Pregnant rats were bred in another cage. The pregnant 47 rats were randomly divided into 2 groups: control group (n =10) and experimental group (n =37). The experimental pregnant rats were intraperitoneally injected with LPS

  17. A Regulatory miRNA–mRNA Network Is Associated with Tissue Repair Induced by Mesenchymal Stromal Cells in Acute Kidney Injury

    Science.gov (United States)

    de Almeida, Danilo Candido; Bassi, Ênio Jose; Azevedo, Hatylas; Anderson, Letícia; Origassa, Clarice Silvia Taemi; Cenedeze, Marcos Antônio; de Andrade-Oliveira, Vinicius; Felizardo, Raphael José Ferreira; da Silva, Reinaldo Correia; Hiyane, Meire Ioshie; Semedo, Patricia; dos Reis, Marlene Antônia; Moreira-Filho, Carlos Alberto; Verjovski-Almeida, Sergio; Pacheco-Silva, Álvaro; Câmara, Niels Olsen Saraiva

    2017-01-01

    Mesenchymal stromal cells (MSCs) orchestrate tissue repair by releasing cell-derived microvesicles (MVs), which, presumably by small RNA species, modulate global gene expression. The knowledge of miRNA/mRNA signatures linked to a reparative status may elucidate some of the molecular events associated with MSC protection. Here, we used a model of cisplatin-induced kidney injury (acute kidney injury) to assess how MSCs or MVs could restore tissue function. MSCs and MVs presented similar protective effects, which were evidenced in vivo and in vitro by modulating apoptosis, inflammation, oxidative stress, and a set of prosurvival molecules. In addition, we observed that miRNAs (i.e., miR-880, miR-141, miR-377, and miR-21) were modulated, thereby showing active participation on regenerative process. Subsequently, we identified that MSC regulates a particular miRNA subset which mRNA targets are associated with Wnt/TGF-β, fibrosis, and epithelial–mesenchymal transition signaling pathways. Our results suggest that MSCs release MVs that transcriptionally reprogram injured cells, thereby modulating a specific miRNA–mRNA network. PMID:28096802

  18. Neurotrophic Effect of Adipose Tissue-Derived Stem Cells on Erectile Function Recovery by Pigment Epithelium-Derived Factor Secretion in a Rat Model of Cavernous Nerve Injury

    Directory of Open Access Journals (Sweden)

    Xin Chen

    2016-01-01

    Full Text Available The paracrine effect is the major mechanism of stem cell therapy. However, the details of the effect’s mechanism remain unknown. The aim of this study is to investigate whether adipose tissue-derived stem cells (ADSCs can ameliorate cavernous nerve injury-induced erectile dysfunction (CNIED rats and to determine its mechanism. Twenty-eight days after intracavernous injection of 5-ethynyl-2-deoxyuridine- (EdU- labeled ADSCs, the erectile function of all the rats was evaluated by intracavernosal pressure (ICP. The ADSCs steadily secreted detectable pigment epithelium-derived factor (PEDF in vitro. The expression of PEDF increased in the penis of the bilateral cavernous nerve injury (BCNI group for 14 days and then gradually decreased. On day 28 after the intracavernous injection, the ADSCs group exhibited a significantly increased ICP compared with the phosphate buffered saline- (PBS- treated group. Moreover, the neuronal nitric oxide synthase (nNOS and S100 expression in penile dorsal nerves and the smooth muscle content to collagen ratio in penile tissues significantly increased. Furthermore, elevated PEDF, p-Akt, and p-eNOS were identified in the ADSCs group. This study demonstrated that intracavernous injection of ADSCs improved erectile function, repaired the nerve, and corrected penile fibrosis. One potential mechanism is the PEDF secretion of ADSCs and subsequent PI3K/Akt pathway activation.

  19. Increased angiotensinogen expression, urinary angiotensinogen excretion, and tissue injury in nonclipped kidneys of two-kidney, one-clip hypertensive rats.

    Science.gov (United States)

    Shao, Weijian; Miyata, Kayoko; Katsurada, Akemi; Satou, Ryousuke; Seth, Dale M; Rosales, Carla B; Prieto, Minolfa C; Mitchell, Kenneth D; Navar, L Gabriel

    2016-08-01

    In angiotensin II (ANG II)-dependent hypertension, there is an angiotensin type 1 receptor-dependent amplification mechanism enhancing intrarenal angiotensinogen (AGT) formation and secretion in the tubular fluid. To evaluate the role of increased arterial pressure, AGT mRNA, protein expression, and urinary AGT (uAGT) excretion and tissue injury were assessed in both kidneys of two-kidney, one-clip Sprague-Dawley hypertensive rats subjected to left renal arterial clipping (0.25-mm gap). By 18-21 days, systolic arterial pressure increased to 180 ± 3 mmHg, and uAGT increased. Water intake, body weights, 24-h urine volumes, and sodium excretion were similar. In separate measurements of renal function in anesthetized rats, renal plasma flow and glomerular filtration rate were similar in clipped and nonclipped kidneys and not different from those in sham rats, indicating that the perfusion pressure to the clipped kidneys remained within the autoregulatory range. The nonclipped kidneys exhibited increased urine flow and sodium excretion. The uAGT excretion was significantly greater in nonclipped kidneys compared with clipped and sham kidneys. AGT mRNA was 2.15-fold greater in the nonclipped kidneys compared with sham (1.0 ± 0.1) or clipped (0.98 ± 0.15) kidneys. AGT protein levels were also greater in the nonclipped kidneys. The nonclipped kidneys exhibited greater glomerular expansion and immune cell infiltration, medullary fibrosis, and cellular proliferation than the clipped kidneys. Because both kidneys have elevated ANG II levels, the greater tissue injury in the nonclipped kidneys indicates that an increased arterial pressure synergizes with increased intrarenal ANG II to stimulate AGT production and exert greater renal injury.

  20. Increased poly(ADP-ribosyl)ation in skeletal muscle tissue of pediatric patients with severe burn injury: prevention by propranolol treatment.

    Science.gov (United States)

    Oláh, Gábor; Finnerty, Celeste C; Sbrana, Elena; Elijah, Itoro; Gerö, Domokos; Herndon, David N; Szabó, Csaba

    2011-07-01

    Activation of the nuclear enzyme poly(ADP-ribose) polymerase (PARP) has been shown to promote cellular energetic collapse and cellular necrosis in various forms of critical illness. Most of the evidence implicating the PARP pathway in disease processes is derived from preclinical studies. With respect to PARP and burns, studies in rodent and large animal models of burn injury have demonstrated the activation of PARP in various tissues and the beneficial effect of its pharmacological inhibition. The aims of the current study were to measure the activation of PARP in human skeletal muscle biopsies at various stages of severe pediatric burn injury and to identify the cell types where this activation may occur. Another aim of the study was to test the effect of propranolol (an effective treatment of patients with burns) on the activation of PARP in skeletal muscle biopsies. Poly(ADP-ribose) polymerase activation was measured by Western blotting for its product, poly(ADP-ribose) (PAR). The localization of PARP activation was determined by PAR immunohistochemistry. The results showed that PARP becomes activated in the skeletal muscle tissue after burns, with the peak of the activation occurring in the middle stage of the disease (13-18 days after burns). Even at the late stage of the disease (69-369 days after burn), an elevated degree of PARP activation persisted in some of the patients. Immunohistochemical studies localized the staining of PAR primarily to vascular endothelial cells and occasionally to resident mononuclear cells. There was a marked suppression of PARP activation in the skeletal muscle biopsies of patients who received propranolol treatment. We conclude that human burn injury is associated with the activation of PARP. We hypothesize that this response may contribute to the inflammatory responses and cell dysfunction in burns. Some of the clinical benefit of propranolol in burns may be related to its inhibitory effect on PARP activation.

  1. Transplantation of bone marrow stromal cells overexpressing human vascular endothelial growth factor 165 enhances tissue repair in a rat model of radiation-induced injury

    Institute of Scientific and Technical Information of China (English)

    Wang Tao; Liao Tian'an; Wang Hong; Deng Wei; Yu Dahai

    2014-01-01

    Background The multilineage differentiation potential ability of bone marrow stromal cells (BMSCs) showed great potential in tissue engineering,while vascular endothelial growth factor 165 (VEGF165) promotes vasculogenesis and further promotes tissue regeneration.This study aimed to assess the ability of rat BMSCs expressing human VEGFA165 (hVEGF165) to promote tissue repair in rat model of radiation-induced injury.Methods Rat BMSCs were isolated from the tibia.Plasmid DNA expressing hVEGF165 was stably transfected into BMSCs using liposomes.The right hindlimb muscle of 40 rats was irradiated using a 60Co y source (total dose 30 Gy).The animals were divided into four groups (n=10):not injected with BMSCs (control; group 1) or intramuscularly injected two times (once in 2 weeks) with pcDNATM3.1-transfected BMSCs (group 2),untransfected BMSCs (group 3),or hVEGF165-transfected BMSCs (group 4).Angiography was performed 1 week after the last injection of BMSCs; samples of the hindlimb muscle were subjected to transmission electron microscopy,ultrastructural analysis,reverse transcription-PCR (RT-PCR),Western blotting,and immunohistochemistry.Results Rat BMSCs with multipotent differentiation capacity were isolated,hVEGF165-transfected BMSCs overexpressed hVEGF165 mRNA and protein.Injection of BMSCs (groups 2-4) increased the average vessel number,density,diameter,and cross-sectional area; mRNA expression of the myogenic markers including myoblast determination protein,myogenin,and α-smooth muscle actin; and CD31 protein expression; and promoted the repair of blood vessels and myofibers after radiation-induced injury compared to group 1; each of these parameters and hVEGF165 mRNA or protein expression were markedly improved in rats injected with hVEGF165-transfected BMSCs compared to groups 2 and 3.Conclusions BMSCs expressing hVEGF165 enhanced the repair of radiation-induced tissue injury by promoting vasculogenesis and muscle fiber regeneration.BMSCs expressing h

  2. Tissue distribution, metabolism and hepatic tissue injury in Chinese lizards (Eremias argus) after a single oral administration of lambda-cyhalothrin.

    Science.gov (United States)

    Chang, Jing; Li, Jitong; Wang, Huili; Wang, Yinghuan; Guo, Baoyuan; Yin, Jing; Hao, Weiyu; Li, Wei; Li, Jianzhong; Xu, Peng

    2016-11-01

    Lambda-cyhalothrin (LCT) is a widely used pyrethroid with neurotoxicity. However, little is known about the toxicokinetics of LCT in reptiles. In this study, the absorption, distribution, metabolism and excretion of LCT in Chinese lizards (Eremias Argus) were determined following a single dose (10 mg kg(-1)) treatment. In the liver, brain, gonads and skin, LCT levels peaked within several hours and then decreased rapidly. However, the concentration of LCT gradually increased in the fat tissue. More than 90% of the LCT dose was excreted in the faeces. One LCT metabolite, 3-phenoxybenzoic acid (PBA), was detected in lizard plasma and tissues. PBA preferentially accumulates in the brain and plasma. The half-life of PBA in the brain was 3.2 days, which was 35.4-fold greater than that of LCT. In the plasma, the concentration of PBA was significantly higher than that of LCT. The bioaccumulation of LCT in tissues was enantioselective, and the enantiomeric fractions (EF) ranged from 0.72 to 0.26. The preferential accumulation of enantiomers changed according to exposure time, but the reasons behind this phenomenon were not clear. For pathological analysis, vacuolation of the cytoplasm and large areas of necrosis were observed in the liver sections after 168 h of dosing. The liver tissues exhibited both decreases in the hepatosomatic index and histopathological lesions during the exposure period. In this study, the effect concentration of LCT in lizards was 200-fold lower than its LD50 value used in risk assessments for birds. These results may provide additional information for the risk assessment of LCT for reptiles and indicate that birds may not be an ideal surrogate for reptile toxicity evaluation.

  3. Experience with Proctectomy to Manage Combat Casualties Sustaining Catastrophic Perineal Blast Injury Complicated by Invasive Mucor Soft-Tissue Infections

    Science.gov (United States)

    2014-03-01

    traumatic amputations, open pelvic fracture with left sacroiliac joint dissociation, traumatic right orchiectomy, penile burns with membranous...transfused within 24 hours of injury).4 A recent clinical practice guideline addressing IFI has been published by the Department of Defense Joint ...1441 9. 5. Department of Defense Joint Theater Trauma System. http://www.usaisr .amedd.army.mil/assets/cpgs/Invasive Fungal Infection in War Wounds 1

  4. A Prospective Randomized Study of Brain Tissue Oxygen Pressure-Guided Management in Moderate and Severe Traumatic Brain Injury Patients

    Directory of Open Access Journals (Sweden)

    Chien-Min Lin

    2015-01-01

    Full Text Available The purpose of this study was to compare the effect of PbtO2-guided therapy with traditional intracranial pressure- (ICP- guided treatment on the management of cerebral variables, therapeutic interventions, survival rates, and neurological outcomes of moderate and severe traumatic brain injury (TBI patients. From 2009 to 2010, TBI patients with a Glasgow coma scale 20 mmHg, and 27 patients were treated with ICP-guided therapy (ICP 60 mmHg in the neurosurgical intensive care unit (NICU; demographic characteristics were similar across groups. The survival rate in the PbtO2-guided group was also significantly increased at 3 and 6 months after injury. Moreover, there was a significant correlation between the PbtO2 signal and Glasgow outcome scale-extended in patients from 1 to 6 months after injury. This finding demonstrates that therapy directed by PbtO2 monitoring is valuable for the treatment of patients with moderate and severe TBI and that increasing PaO2 to 150 mmHg may be efficacious for preventing cerebral hypoxic events after brain trauma.

  5. First molluscan transcription factor activator protein-1 (Ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury.

    Science.gov (United States)

    De Zoysa, Mahanama; Nikapitiya, Chamilani; Lee, Youngdeuk; Lee, Sukkyoung; Oh, Chulhong; Whang, Ilson; Yeo, Sang-Yeop; Choi, Cheol Young; Lee, Jehee

    2010-12-01

    The regulation of transcriptional activation is an essential and critical point in gene expression. In this study, we describe a novel transcription factor activator protein-1 (Ap-1) gene from disk abalone Haliotis discus discus (AbAp-1) for the first time in mollusk. It was identified by homology screening of an abalone normalized cDNA library. The cloned AbAp-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. The AbAp-1 gene is composed of a characteristic Jun transcription factor domain and a highly conserved basic leucine zipper (bZIP) signature similar to known Ap-1 genes. The AbAp-1 shares 46, 43 and, 40% amino acid identities with fish (Takifugu rubripes), human and insect (Ixodes scapularis) Ap-1, respectively. Quantitative real time RT-PCR analysis confirmed that AbAp-1 gene expression is constitutive in all selected tissues. AbAp-1 was upregulated in gills after bacteria (Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes) challenge; and, upregulated in hemocytes and gills by viral hemorrhagic septicemia virus (VHSV) challenge. Shell damage and tissue injury also increased the transcriptional level of Ap-1 in mantle together with other transcription factors (NF-kB, LITAF) and pro-inflammatory TNF-α. All results considered, identification and gene expression data demonstrate that abalone Ap-1 is an important regulator in innate immune response against bacteria and virus, as well as in the inflammatory response during tissue injury. In addition, stimulation of Ap-1 under different external stimuli could be useful to understand the Ap-1 biology and its downstream target genes, especially in abalone-like mollusks.

  6. Protective effects of β-glucan against oxidative injury induced by 2.45-GHz electromagnetic radiation in the skin tissue of rats.

    Science.gov (United States)

    Ceyhan, Ali Murat; Akkaya, Vahide Baysal; Güleçol, Şeyma Celik; Ceyhan, Betül Mermi; Özgüner, Fehmi; Chen, WenChieh

    2012-09-01

    In recent times, there is widespread use of 2.45-GHz irradiation-emitting devices in industrial, medical, military and domestic application. The aim of the present study was to investigate the effect of 2.45-GHz electromagnetic radiation (EMR) on the oxidant and antioxidant status of skin and to examine the possible protective effects of β-glucans against the oxidative injury. Thirty-two male Wistar albino rats were randomly divided into four equal groups: control; sham exposed; EMR; and EMR + β-glucan. A 2.45-GHz EMR emitted device from the experimental exposure was applied to the EMR group and EMR + β-glucan group for 60 min daily, respectively, for 4 weeks. β-glucan was administered via gavage at a dose of 50 mg/kg/day before each exposure to radiation in the treatment group. The activities of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), as well as the concentration of malondialdehyde (MDA) were measured in tissue homogenates of the skin. Exposure to 2.45-GHz EMR caused a significant increase in MDA levels and CAT activity, while the activities of SOD and GSH-Px decreased in skin tissues. Systemic β-glucan significantly reversed the elevation of MDA levels and the reduction of SOD activities. β-glucan treatment also slightly enhanced the activity of CAT and prevented the depletion of GSH-Px activity caused by EMR, but not statistically significantly. The present study demonstrated the role of oxidative mechanisms in EMR-induced skin tissue damages and that β-glucan could ameliorate oxidative skin injury via its antioxidant properties.

  7. 腹腔镜下子宫切除术治疗子宫腺肌病的临床疗效分析%Clinical Analysis of Laparoscopic Hysterectomy in the Treatment of Adenomyosis

    Institute of Scientific and Technical Information of China (English)

    余洋; 王和

    2013-01-01

    West China Second University Hospital. Informed consent was obtained from all participates. The following items were observed retrospectively, surgical indicators (operation duration, blood loss and postoperative blood transfusion situation), indicators of short-term recovery (postoperative indwelling catheter time, placement of negative pressure drainage, anal exhaust time, postoperative pain score, and hospital stay), and indicators of long-term follow-up (alleviation situation of postoperative dysmenorrheal, assessment of sexual life quality, quality evaluation of daily life, pelvic floor tissue support, lesions and malignant transformation rate of cervical stump, as well as the existence of persistent postoperative cyclical vaginal bleeding). Results The operation duration, blood loss of LSH group were less than those in LTH group, with significant difference (P0. 001). Dysmenorrhea symptoms of two groups were remission, and the incidence of postoperative complications were lower. Conclusions The clinical effects of LSH group is better than that of LTH group, and LSH is recommended in treating adenomyosis.

  8. LNG-IUS对腺肌症子宫内膜VEGF、Ang-1、Ang-2的影响%The influence of levonorgestrel intrauterine system on endometrial VEGF/Ang-1/Ang-2 of women with adenomyosis

    Institute of Scientific and Technical Information of China (English)

    姚佳娜; 谢梅青

    2013-01-01

    Objective To determine the alteration of endometrial VEGF/Ang-1/Ang-2 expression of women with adenomyosis after using levonogestrel intrauterine system,and elucidate the possible mechanism of breakthrough bleeding in the use of LNG-IUS on angiogenesis and vascular stability.Methods Endometrial biopsies was obtained from 41 women with adenomyosis undergoing treatment with LNG-IUS by using Pipelle suction curette,determine the endometrial VEGF/Ang-1/Ang-2 expression through immunohistochemistry.We also took the endometrial tissue from patients diagnosed as adenomyosis without any treatment as control groups.Results The LNG-IUS group was divided into bleeding group (16 cases) and non-bleeding group (25 cases),according to the bleeding patterns in the last 3 months before biopsy.The staining for VEGF/Ang-2 in bleeding group was significantly higher than that in nonbleeding group (P<0.05).The Ang-1 staining in glandular epithelium and stromal cells between two groups was not significant different (P>0.05).Divided the LNG-IUS group into the less than 1-year group,1-2 year group and more than 2-year group,we had found that the 1-2 year group and the more than 1-year group exhibited significantly lower immunoreactivity for VEGF and Ang-2 in both glandular epithelium and stromal cells (P<0.05),while there were no significant differences in immunoreactivity for the Ang-1 among different groups (P>0.05).Conclusion VEGF and Ang1,Ang-2 may play a role in provoking breakthrough bleeding in the use of LNG-IUS by influencing angiogenesis and vascular stability.%目的 研究子宫腺肌症患者放置左炔诺孕酮宫内缓释系统(LNG-IUS)后子宫内膜血管内皮生长因子(VEGF)、促血管生成素(Ang-1、Ang-2)表达的变化,从血管生成及血管稳定性方面探讨子宫腺肌症患者放置LNG-IUS后不规则出血的发生机制.方法 收集41例放置LNG-IUS腺肌症患者的子宫内膜,应用免疫组化染色(DAKO EnvisionTM System

  9. [Clinical study of high intensity focused ultrasound ablation combined with GnRH-a and LNG-IUS for the treatment of adenomyosis].

    Science.gov (United States)

    Ye, M Z; Deng, X L; Zhu, X G; Xue, M

    2016-09-25

    Objective: To investigate the clinical effect of dysmenorrhea in patients with adenomyosis treated by high intensity focused ultrasound(HIFU)ablation combined with gonadotropin-releasing hormone agonist(GnRH-a)and levonorgestrel-releasing intrauterine system(LNG-IUS). Methods: From April 2012 to December 2015, 477 cases of adenomyosis patients with dysmenorrhea were treated by HIFU in the Third Xiangya Hospital. Among them, some patients were treated with HIFU alone, some of them were treated with HIFU combined with GnRH-a and(or)LNG-IUS, thus were classified as H group, H+G group, H+M group and H+G+M group. The improvements of clinical results were compared among the four groups and the influencing factors of HIFU treatment for adenomyosis were also analyzed. Results: During the follow-up period, the overall effective rates of the treatment decreased with time, 3 months 89.4%(345/386), 12 months 84.0%(221/263), 24 months 74.2%(98/132), and the overall recurrence rate was 12.9%(39/303). The significant difference in the curative at 3 months[H group 83.7%(170/203), H+M group 95.0%(95/100), H+G group 100.0%(43/43), H+G+M group 96.8%(30/31)], 12 months[H group 79.4%(123/155), H+M group 93.2%(69/74), H+G group 11/12, H+G+M group 15/17], and 24 months[H group 68.0%(51/75), H+M group 96.4%(27/28), H+G group 6/12, H+G+M group 15/15]after HIFU treatment and recurrence rate[H group 19.0%(29/153), H+M group 3.3%(3/90), H+G group 19.4%(6/31), H+G+M group 4.5%(1/22)]were observed among the four groups(PLNG-IUS could improve the treatment effect in relief of dysmenorrhea. Based on our results, individual treatment protocol should be selected for different patients.

  10. Evaluation of the susceptibility artifacts and tissue injury caused by implanted microchips in dogs on 1.5 T magnetic resonance imaging.

    Science.gov (United States)

    Saito, Miyoko; Ono, Shin; Kayanuma, Hideki; Honnami, Muneki; Muto, Makoto; Une, Yumi

    2010-05-01

    Performing magnetic resonance imaging (MRI) in patients with a metallic implant raises concern over the potential complications, including susceptibility artifacts, implant migration, and heat injury. The purpose of this study was to investigate these complications in dogs with implanted microchips by evaluating MR images and the histopathological changes after 1.5 Tesla (T) MRI. Five dogs underwent microchip implantation in the cervicothoracic area. One month later, the area was imaged using 1.5T MRI in three dogs. The microchips were removed surgically together with the surrounding tissue in all dogs. There was significant signal loss and image distortion over a wide range around the area where the microchip was implanted. This change was consistent with susceptibility artifacts, which rendered the affected area including the spinal cord undiagnostic. The artifact was more extensive in T2*-weighted images (gradient-echo) and less extensive in proton density-weighted images (fast spin-echo with short echo time). Histopathologically, all microchips were well-encapsulated with granulation tissue, and there were no evidence of migration of microchips. Cell debris and a moderate number of degenerated cells with fibrin were seen in the inner layer of the granulation tissue in each dog that underwent MRI. These changes were very subtle and did not seem to be clinically significant. The results of this study suggest that, in 1.5T MRI, susceptibility artifacts produced by implanted microchips can be marked, although the dogs with implants appeared to be scanned safely.

  11. Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation

    Directory of Open Access Journals (Sweden)

    Dongdong Liu

    2014-01-01

    Full Text Available Acute respiratory distress syndrome (ARDS remains a high morbidity and mortality disease entity in critically ill patients, despite decades of numerous investigations into its pathogenesis. To obtain global protein expression changes in acute lung injury (ALI lung tissues, we employed a high-throughput proteomics method to identify key components which may be involved in the pathogenesis of ALI. In the present study, we analyzed lung tissue proteomes of Pseudomonas aeruginosa-induced ALI rats and identified eighteen proteins whose expression levels changed more than twofold as compared to normal controls. In particular, we found that PRDX1 expression in culture medium was elevated by a lipopolysaccharide (LPS challenge in airway epithelial cells in vitro. Furthermore, overexpression of PRDX1 increased the expression of proinflammatory cytokines interleukin-6 (IL-6, interleukin-8 (IL-8, and tumor necrosis factor-α (TNF-α, whereas knockdown of PRDX1 led to downregulated expression of cytokines induced by LPS. In conclusion, our findings provide a global alteration in the proteome of lung tissues in the ALI rat model and indicate that PRDX1 may play a critical role in the pathogenesis of ARDS by promoting inflammation and represent a novel strategy for the development of new therapies against ALI.

  12. Thromboxane A{sub 2} receptor signaling promotes liver tissue repair after toxic injury through the enhancement of macrophage recruitment

    Energy Technology Data Exchange (ETDEWEB)

    Minamino, Tsutomu [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Departments of Gastroenterology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Ito, Yoshiya [Departments of Surgery, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Ohkubo, Hirotoki [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Departments of Surgery, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Hosono, Kanako; Suzuki, Tatsunori [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Sato, Takehito [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Departments of Gastroenterology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Ae, Takako; Shibuya, Akitaka [Departments of Gastroenterology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Sakagami, Hiroyuki [Departments of Anatomy, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Narumiya, Shuh [Department of Pharmacology, Kyoto University School of Medicine, Kyoto, 606-8315 (Japan); Koizumi, Wasaburo [Departments of Gastroenterology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan); Majima, Masataka, E-mail: mmajima@med.kitasato-u.ac.jp [Departments of Pharmacology, Kitasato University School of Medicine, Kanagawa 252-0374 (Japan)

    2012-02-15

    It is thought that thromboxane A{sub 2} (TxA{sub 2}) contributes to the progression of inflammation during acute hepatic injury; however, it is still unknown whether TxA{sub 2} is involved in liver repair. The objective of the present study was to examine the role of TxA{sub 2} receptor (TP) signaling in liver injury and repair in response to toxic injury. Carbon tetrachloride (CCl{sub 4}) was used to induce liver injury in TP knockout (TP{sup −/−}) mice and wild-type (WT) mice. In WT mice, serum levels of alanine aminotransferase (ALT) and the size of the necrotic area peaked at 24 and 48 h, respectively, and then declined. In TP{sup −/−} mice, the changes in ALT levels were similar to WT mice, but liver regeneration was impaired as evidenced by remained elevated levels of hepatic necrosis and by delayed hepatocyte proliferation, which was associated with the reduced expression of growth factors including interleukin-6 (IL-6), tumor necrosis factor alpha (TNFα), and hepatocyte growth factor (HGF). In TP{sup −/−} mice, the accumulation of hepatic CD11b{sup +}/F4/80{sup +} macrophages in injured livers was attenuated, and the hepatic expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and its receptor, the C―C chemokine receptor (CCR2), was reduced compared to WT. Additionally, the application of the TP receptor agonist, U-46619, enhanced the expression of MCP-1/CCL2 and CCR2 in peritoneal macrophages, which was associated with increased levels of IL-6, TNFα and HGF. These results suggested that TP receptor signaling facilitates liver recovery following CCl{sub 4}-induced hepatotoxicity by affecting the expression of hepatotrophic growth factors, and through the recruitment of macrophages mediated by MCP-1/CCL2-CCR2 expression. -- Highlights: ► TP enhances liver regeneration by CCl{sub 4}. ► TP accumulates macrophages. ► TP up-regulates MCP-1.

  13. Study on changes of partial pressure of brain tissue oxygen and brain temperature in acute phase of severe head injury during mild hypothermia therapy

    Institute of Scientific and Technical Information of China (English)

    朱岩湘; 姚杰; 卢尚坤; 章更生; 周关仁

    2003-01-01

    Objective: To study the changes of partial pressure of brain tissue oxygen (PbtO2) and brain temperature in acute phase of severe head injury during mild hypothermia therapy and the clinical significance.Methods: One hundred and sixteen patients with severe head injury were selected and divided into a mild hypothermia group (n=58), and a control group (n=58) according to odd and even numbers of hospitalization. While mild hypothermia therapy was performed PbtO2 and brain temperature were monitored for 1-7 days (mean=86 hours), simultaneously, the intracranial pressure, rectum temperature, cerebral perfusion pressure, PaO2 and PaCO2 were also monitored. The patients were followed up for 6 months and the prognosis was evaluated with GOS (Glasgow outcome scale).Results: The mean value of PbtO2 within 24 hour monitoring in the 116 patients was 13.7 mm Hg±4.94 mm Hg, lower than the normal value (16 mm Hg±40 mm Hg) The time of PbtO2 recovering to the normal value in the mild hypothermia group was shortened by 10±4.15 hours compared with the control group (P<0.05). The survival rate of the mild hypothermia group was 60.43%, higher than that of the control group (46.55%). After the recovery of the brain temperature, PbtO2 increased with the rise of the brain temperature. Conclusions: Mild hypothermia can improve the survival rate of severe head injury. The technique of monitoring PbtO2 and the brain temperature is safe and reliable, and has important clinical significance in judging disease condition and instructing clinical therapy.

  14. RIPK3 deficiency or catalytically inactive RIPK1 provides greater benefit than MLKL deficiency in mouse models of inflammation and tissue injury

    Science.gov (United States)

    Newton, K; Dugger, D L; Maltzman, A; Greve, J M; Hedehus, M; Martin-McNulty, B; Carano, R A D; Cao, T C; van Bruggen, N; Bernstein, L; Lee, W P; Wu, X; DeVoss, J; Zhang, J; Jeet, S; Peng, I; McKenzie, B S; Roose-Girma, M; Caplazi, P; Diehl, L; Webster, J D; Vucic, D

    2016-01-01

    Necroptosis is a caspase-independent form of cell death that is triggered by activation of the receptor interacting serine/threonine kinase 3 (RIPK3) and phosphorylation of its pseudokinase substrate mixed lineage kinase-like (MLKL), which then translocates to membranes and promotes cell lysis. Activation of RIPK3 is regulated by the kinase RIPK1. Here we analyze the contribution of RIPK1, RIPK3, or MLKL to several mouse disease models. Loss of RIPK3 had no effect on lipopolysaccharide-induced sepsis, dextran sodium sulfate-induced colitis, cerulein-induced pancreatitis, hypoxia-induced cerebral edema, or the major cerebral artery occlusion stroke model. However, kidney ischemia–reperfusion injury, myocardial infarction, and systemic inflammation associated with A20 deficiency or high-dose tumor necrosis factor (TNF) were ameliorated by RIPK3 deficiency. Catalytically inactive RIPK1 was also beneficial in the kidney ischemia–reperfusion injury model, the high-dose TNF model, and in A20−/− mice. Interestingly, MLKL deficiency offered less protection in the kidney ischemia–reperfusion injury model and no benefit in A20−/− mice, consistent with necroptosis-independent functions for RIPK1 and RIPK3. Combined loss of RIPK3 (or MLKL) and caspase-8 largely prevented the cytokine storm, hypothermia, and morbidity induced by TNF, suggesting that the triggering event in this model is a combination of apoptosis and necroptosis. Tissue-specific RIPK3 deletion identified intestinal epithelial cells as the major target organ. Together these data emphasize that MLKL deficiency rather than RIPK1 inactivation or RIPK3 deficiency must be examined to implicate a role for necroptosis in disease. PMID:27177019

  15. Phosphorylated Histone 3 at Serine 10 Identifies Activated Spinal Neurons and Contributes to the Development of Tissue Injury-Associated Pain

    Science.gov (United States)

    Torres-Pérez, Jose Vicente; Sántha, Péter; Varga, Angelika; Szucs, Peter; Sousa-Valente, Joao; Gaal, Botond; Sivadó, Miklós; Andreou, Anna P; Beattie, Sara; Nagy, Bence; Matesz, Klara; C. Arthur, J. Simon; Jancsó, Gábor; Nagy, Istvan

    2017-01-01

    Transcriptional changes in superficial spinal dorsal horn neurons (SSDHN) are essential in the development and maintenance of prolonged pain. Epigenetic mechanisms including post-translational modifications in histones are pivotal in regulating transcription. Here, we report that phosphorylation of serine 10 (S10) in histone 3 (H3) specifically occurs in a group of rat SSDHN following the activation of nociceptive primary sensory neurons by burn injury, capsaicin application or sustained electrical activation of nociceptive primary sensory nerve fibres. In contrast, brief thermal or mechanical nociceptive stimuli, which fail to induce tissue injury or inflammation, do not produce the same effect. Blocking N-methyl-D-aspartate receptors or activation of extracellular signal-regulated kinases 1 and 2, or blocking or deleting the mitogen- and stress-activated kinases 1 and 2 (MSK1/2), which phosphorylate S10 in H3, inhibit up-regulation in phosphorylated S10 in H3 (p-S10H3) as well as fos transcription, a down-stream effect of p-S10H3. Deleting MSK1/2 also inhibits the development of carrageenan-induced inflammatory heat hyperalgesia in mice. We propose that p-S10H3 is a novel marker for nociceptive processing in SSDHN with high relevance to transcriptional changes and the development of prolonged pain. PMID:28120884

  16. Use of diffusion tensor imaging to assess the impact of normobaric hyperoxia within at-risk pericontusional tissue after traumatic brain injury.

    Science.gov (United States)

    Veenith, Tonny V; Carter, Eleanor L; Grossac, Julia; Newcombe, Virginia F; Outtrim, Joanne G; Nallapareddy, Sridhar; Lupson, Victoria; Correia, Marta M; Mada, Marius M; Williams, Guy B; Menon, David K; Coles, Jonathan P

    2014-10-01

    Ischemia and metabolic dysfunction remain important causes of neuronal loss after head injury, and we have shown that normobaric hyperoxia may rescue such metabolic compromise. This study examines the impact of hyperoxia within injured brain using diffusion tensor imaging (DTI). Fourteen patients underwent DTI at baseline and after 1 hour of 80% oxygen. Using the apparent diffusion coefficient (ADC) we assessed the impact of hyperoxia within contusions and a 1 cm border zone of normal appearing pericontusion, and within a rim of perilesional reduced ADC consistent with cytotoxic edema and metabolic compromise. Seven healthy volunteers underwent imaging at 21%, 60%, and 100% oxygen. In volunteers there was no ADC change with hyperoxia, and contusion and pericontusion ADC values were higher than volunteers (P<0.01). There was no ADC change after hyperoxia within contusion, but an increase within pericontusion (P<0.05). We identified a rim of perilesional cytotoxic edema in 13 patients, and hyperoxia resulted in an ADC increase towards normal (P=0.02). We demonstrate that hyperoxia may result in benefit within the perilesional rim of cytotoxic edema. Future studies should address whether a longer period of hyperoxia has a favorable impact on the evolution of tissue injury.

  17. Relative Tissue Factor Deficiency Attenuates Ventilator-Induced Coagulopathy but Does Not Protect against Ventilator-Induced Lung Injury in Mice

    Directory of Open Access Journals (Sweden)

    Esther K. Wolthuis

    2012-01-01

    Full Text Available Preventing tissue-factor-(TF- mediated systemic coagulopathy improves outcome in models of sepsis. Preventing TF-mediated pulmonary coagulopathy could attenuate ventilator-induced lung injury (VILI. We investigated the effect of relative TF deficiency on pulmonary coagulopathy and inflammation in a murine model of VILI. Heterozygous TF knockout (TF+/− mice and their wild-type (TF+/+ littermates were sedated (controls or sedated, tracheotomized, and mechanically ventilated with either low or high tidal volumes for 5 hours. Mechanical ventilation resulted in pulmonary coagulopathy and inflammation, with more injury after mechanical ventilation with higher tidal volumes. Compared with TF+/+ mice, TF+/− mice demonstrated significantly lower pulmonary thrombin-antithrombin complex levels in both ventilation groups. There were, however, no differences in lung wet-to-dry ratio, BALF total protein levels, neutrophil influx, and lung histopathology scores between TF+/− and TF+/+ mice. Notably, pulmonary levels of cytokines were significantly higher in TF+/− as compared to TF+/+ mice. Systemic levels of cytokines were not altered by the relative absence of TF. TF deficiency is associated with decreased pulmonary coagulation independent of the ventilation strategy. However, relative TF deficiency does not reduce VILI and actually results in higher pulmonary levels of inflammatory mediators.

  18. Changes of mature adipocytes after incision injury of subcutaneous adipose tissue%皮下脂肪组织创伤后脂肪细胞的变化

    Institute of Scientific and Technical Information of China (English)

    钱雄; 林炜栋; 江万里; 刘伟伟; 原博; 陈向芳

    2012-01-01

    目的 观察脂肪组织创伤愈合的情况,探讨成熟脂肪细胞的变化.方法 以杜洛克母猪为实验动物,用滚轴取皮刀在其背部制作脂肪组织切割伤皮瓣,大体观察伤后1、2、4及12周的愈合情况.并于伤后3、7、14及21 d在皮瓣中央处取材,观察切缘及周围成熟脂肪细胞的形态学改变,用酶联免疫吸附法和免疫组织化学方法检测脂肪组织中瘦素和脂联素的表达情况.结果 脂肪组织切割伤组皮瓣中央与未创伤处皮肤无异,但皮瓣边缘早期有明显炎症反应,后期则呈典型的疤痕生长.组织学观察发现:脂肪组织创伤后,仍以纤维化愈合为主,但愈合过程中,炎症反应的局灶性及小脂肪细胞出现呈现一定的规律性;与未创伤组相比,脂肪组织创伤后其瘦素及脂联素的表达量均呈下降趋势,但两者的比值在愈合的过程中存在差异.结论 脂肪组织创伤后,脂肪细胞表面瘦素及脂联素的表达量下降.早期创缘附近出现大量的小脂肪细胞,可能与脂肪分解有关.%Objective To investigate the changes of mature adipocytes after incision injury in subcutaneous adipose tissue. Methods Wound model was induced by incised flaps on the back of female red Duroc pigs (FRDP) using roller dermatome. The megascopic morphological changes of flaps were observed at week 1,2,4 and 12. The samples in the center of flaps were harvested at day 3,7, 14 and 21; the morphological changes of incisal edge and associated mature adipocytes were observed; the expression of leptin and adiponectin of incised subcutaneous adipose tissue was detected by imrnunohistochemistry and ELISA methods. Results The center of flaps in incised adipose tissue showed no difference compared with the unwounded tissue, but the evident inflammation developed in its edges and resolved in scar formation eventually. Furthermore, histological outcomes of wound areas in adipose tissue revealed the collagen deposition and

  19. FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration.

    Directory of Open Access Journals (Sweden)

    Alessandra Castiglioni

    Full Text Available Muscle injury induces a classical inflammatory response in which cells of the innate immune system rapidly invade the tissue. Macrophages are prominently involved in this response and required for proper healing, as they are known to be important for clearing cellular debris and supporting satellite cell differentiation. Here, we sought to assess the role of the adaptive immune system in muscle regeneration after acute damage. We show that T lymphocytes are transiently recruited into the muscle after damage and appear to exert a pro-myogenic effect on muscle repair. We observed a decrease in the cross-sectional area of regenerating myofibers after injury in Rag2-/- γ-chain-/- mice, as compared to WT controls, suggesting that T cell recruitment promotes muscle regeneration. Skeletal muscle infiltrating T lymphocytes were enriched in CD4+CD25+FOXP3+ cells. Direct exposure of muscle satellite cells to in vitro induced Treg cells effectively enhanced their expansion, and concurrently inhibited their myogenic differentiation. In vivo, the recruitment of Tregs to acutely injured muscle was limited to the time period of satellite expansion, with possibly important implications for situations in which inflammatory conditions persist, such as muscular dystrophies and inflammatory myopathies. We conclude that the adaptive immune system, in particular T regulatory cells, is critically involved in effective skeletal muscle regeneration. Thus, in addition to their well-established role as regulators of the immune/inflammatory response, T regulatory cells also regulate the activity of skeletal muscle precursor cells, and are instrumental for the proper regeneration of this tissue.

  20. FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration.

    Science.gov (United States)

    Castiglioni, Alessandra; Corna, Gianfranca; Rigamonti, Elena; Basso, Veronica; Vezzoli, Michela; Monno, Antonella; Almada, Albert E; Mondino, Anna; Wagers, Amy J; Manfredi, Angelo A; Rovere-Querini, Patrizia

    2015-01-01

    Muscle injury induces a classical inflammatory response in which cells of the innate immune system rapidly invade the tissue. Macrophages are prominently involved in this response and required for proper healing, as they are known to be important for clearing cellular debris and supporting satellite cell differentiation. Here, we sought to assess the role of the adaptive immune system in muscle regeneration after acute damage. We show that T lymphocytes are transiently recruited into the muscle after damage and appear to exert a pro-myogenic effect on muscle repair. We observed a decrease in the cross-sectional area of regenerating myofibers after injury in Rag2-/- γ-chain-/- mice, as compared to WT controls, suggesting that T cell recruitment promotes muscle regeneration. Skeletal muscle infiltrating T lymphocytes were enriched in CD4+CD25+FOXP3+ cells. Direct exposure of muscle satellite cells to in vitro induced Treg cells effectively enhanced their expansion, and concurrently inhibited their myogenic differentiation. In vivo, the recruitment of Tregs to acutely injured muscle was limited to the time period of satellite expansion, with possibly important implications for situations in which inflammatory conditions persist, such as muscular dystrophies and inflammatory myopathies. We conclude that the adaptive immune system, in particular T regulatory cells, is critically involved in effective skeletal muscle regeneration. Thus, in addition to their well-established role as regulators of the immune/inflammatory response, T regulatory cells also regulate the activity of skeletal muscle precursor cells, and are instrumental for the proper regeneration of this tissue.

  1. 子宫腺肌病的非手术治疗研究进展%Non-surgical treatment of adenomyosis

    Institute of Scientific and Technical Information of China (English)

    陈燕; 周洪贵

    2013-01-01

    The adenomyosis is a common kind of benign lesions of department of gynecology, surgery is traditional treatment, drug supplemented. But injure of hysterectomy was great, that brought psychological distress to patients. As medical technology advances, non-surgical treatment gradually has become the preferred treatment, including medication, uterine artery embolization, high intensity focused ultrasound and so on, each have advantages and disadvantages. The high intensity focused ultrasound, which is a kind of scientific, noninvasive, safe and effective treatment method, and no severe complications, is expected to become the first choice treatment of uterine adenomyosis.%子宫腺肌病是妇科常见的一种良性病变,传统的治疗方法为手术为主、药物为辅。手术切除子宫创伤大、不能保留子宫,给患者带来身体及心理的极大创伤。随着医疗技术进步,非手术治疗方式逐渐成为首选治疗方式,包括药物治疗、子宫动脉栓塞、高强度聚焦超声等。每种治疗方式各有优缺点,而高强度聚焦超声是一种科学、无创、安全有效的治疗方法,近期疗效显著,有望成为子宫腺肌病的首选治疗方法。

  2. Long-term existence of cerebral hypoxic tissue in a rat model of cerebral ischemia/reperfusion injury

    Institute of Scientific and Technical Information of China (English)

    Yidong Wang; Jingrui Pan; Yu Qiu; Xiangpen Li; Mei Li; Ying Peng

    2009-01-01

    BACKGROUND: Hypoxic tissue surrounding the ischemic core may represent the ischemic penumbra following cerebral infarction. However, some studies have shown that the duration of ischemic tissue is longer than previously believed.OBJECTIVE: To clarify whether cerebral hypoxic tissue could survive long-term and whether it is altered in rats following cerebral infarction; to establish an ischemia/reperfusion model in which hypoxic tissue exists for extended periods of time.DESIGN, TIME AND SETTING: A completely randomized grouping and controlled experiment was performed at the Experimental Animal Center of Sun Yat-sen University and Medical Research Center, the Second Affiliated Hospital of Sun Yat-sen University between June and December 2008. MATERIALS: 4,9-diaza-3,3,10,10-tetramethyldodecan-2, 11-dione dioxime (BnAO) (HL91), used as the hypoxic marker for autoradiography, was supplied by the Beijing Syncor Star Medicinal, China, and the flesh eluent Na99TcmO4 to mark HL91 was supplied by Guangzhou Medical Isotope Center of the China Institute of Atomic Energy. 2-(2-nitro-1H-imidazole-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide (EF5) and its antibody ELK3-51, used as a hypoxic marker for immunofluorescence, were supplied by the University of Pennsylvania, USA.METHODS: Male Sprague Dawley rats were randomly divided into four groups: 1.5-hour ischemia/reperfusion group (1.5 h IR), 2-hour ischemia/reperfusion group (2 h IR), 3-hour ischemia/reperfusion group (3 h IR), and permanent ischemia (PI) group, with 21 rats in each group. The middle cerebral artery occlusion model was established using the intraluminal suture method, while reperfusion was performed by removing the suture at each observation time point. However, in the PI group, the suture was left in the artery.MAIN OUTCOME MEASURES: Area and average absorbance of fluorescence, representing hypoxic tissue, were measured by image-analysis.RESULTS: Autoradiography revealed positive hypoxia at days 1 and 14

  3. Effect of mifepristone on the expression of β-endorphin the ectopic focus of adenomyosis%米非司酮对子宫腺肌病异位病灶组织β-内啡肽表达的影响

    Institute of Scientific and Technical Information of China (English)

    王绍光; 姜学强

    2011-01-01

    目的 探讨米非司酮对子宫腺肌病异位病灶组织B-内啡肽表达的影响及其与痛经的关系.方法 应用免疫组化法检测子宫腺肌病异位病灶组织中β-内啡肽的表达.结果 轻度痛经组异位内膜组织β-内啡肽在增生期和分泌期的表达水平均高于中度痛经组(t=2.672、2.711,均P<0.05),中度痛经组异位内膜组织β-内啡肽在增生期和分泌期的表达水平均高于重度痛经组(t=2.341、2.365,均P<0.05).米非司酮治疗后,子宫腺肌病异位内膜组织β-内啡肽在增生期和分泌期的表达均高于子宫腺肌病对照组(t=2.478、2.356,均P<0.05),但均低于正常子宫内膜(t=2.123、2.233,均P<0.05).结论 子宫腺肌病异位病灶中β-内啡肽的表达随着痛经程度的加重而下降,米非司酮可以升调β-内啡肽,缓解痛经.%Objective To investigate the effect of mifepristone on the expression of β-endorphin in the ectopic focus of adenomyosis.Methods The expression of β-endorphin was measured by the immunochemistry method.Results During proliferative phase and secretory phase,the β-endorphin of mild dysmenorrhea were higher than that of midrange dysmenorrhea(t=2.672,2.711,all P<0.05),the β-endorphin of midrange dysmenorrhea was higher than that of severe dysmenorrhea(t=2.341,2.365,all P<0.05),the difference was significant.After being cured by mifepfistone,the expression of β-endorphin in the ectopic focus of adenomyosis was higher than that of control group (t=2.478,2.356,all P<0.05),still lower than that of normal endometrial tissue,(t=2.123,2.233,all P<0.05),the difference was significant.Conclusion The expression of β-endorphin in the ectopic focus of adenomyosis decreased significandy accompanied with the aggravation of the dysmenorrhea,and the mifepfistone could upregulate the β-endorphin and relieve the dysmenorrhea.

  4. The restrained expression of NF-kB in renal tissue ameliorates folic acid induced acute kidney injury in mice.

    Directory of Open Access Journals (Sweden)

    Dev Kumar

    Full Text Available The Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB represent family of structurally-related eukaryotic transcription factors which regulate diverse array of cellular processes including immunological responses, inflammation, apoptosis, growth & development. Increased expression of NF-kB has often been seen in many diverse diseases, suggesting the importance of genomic deregulation to disease pathophysiology. In the present study we focused on acute kidney injury (AKI, which remains one of the major risk factor showing a high rate of mortality and morbidity. The pathology associated with it, however, remains incompletely known though inflammation has been reported to be one of the major risk factor in the disease pathophysiology. The role of NF-kB thus seemed pertinent. In the present study we show that high dose of folic acid (FA induced acute kidney injury (AKI characterized by elevation in levels of blood urea nitrogen & serum creatinine together with extensive tubular necrosis, loss of brush border and marked reduction in mitochondria. One of the salient observations of this study was a coupled increase in the expression of renal, relA, NF-kB2, and p53 genes and proteins during folic acid induced AKI (FA AKI. Treatment of mice with NF-kB inhibitor, pyrrolidine dithio-carbamate ammonium (PDTC lowered the expression of these transcription factors and ameliorated the aberrant renal function by decreasing serum creatinine levels. In conclusion, our results suggested that NF-kB plays a pivotal role in maintaining renal function that also involved regulating p53 levels during FA AKI.

  5. Platelet-Associated CD40/CD154 Mediates Remote Tissue Damage After Mesenteric Ischemia/Reperfusion Injury

    Science.gov (United States)

    2012-02-27

    granules in resting platelets and when platelets are activated CD154 translocates to the membrane for up to 90 minutes prior to its being where it is...endothelial cells. Circulation 106: 2111 2117. 17. Rendu F, Brohard-Bohn B (2001) The platelet release reaction: granules ’ constituents, secretion and...01 FEB 2015 2. REPORT TYPE N/A 3. DATES COVERED - 4. TITLE AND SUBTITLE Platelet -associated CD40/CD154 mediates remote tissue damage after

  6. Throwing Injuries of the Shoulder.

    Science.gov (United States)

    McCue, Frank C., III; and Others

    The majority of shoulder injuries occurring in throwing sports involve the soft tissue structures. Injuries often occur when the unit is overstretched to a point near its greatest length, involving the elastic tissues. The other injury mechanism involves the contractural unit of the muscle, which occurs near the midpoint of contractions, involving…

  7. 围绝经期子宫肌瘤及子宫腺肌病所致异常出血%Perimenopausal abnormal bleeding caused by uterine fibroids and uterine adenomyosis

    Institute of Scientific and Technical Information of China (English)

    汪利群; 黄欧平

    2012-01-01

    Uterine fibroids and adenomyosis is the most common benign gynecological diseases, they are one important reason for abnormal uterine bleeding in perimenopausal period. This article summarizes the e-tiology, diagnosis and treatment of abnormal uterine bleeding caused by fibroids and adenomyosis in perimenopausal women,which should lead to attention.%子宫肌瘤与子宫腺肌病是妇科最常见的良性肿瘤,是导致围绝经期子宫异常出血的重要原因之一.该文概述了子宫肌瘤与子宫腺肌病导致围绝经期异常子宫出血病因、诊断及治疗,以期引起关注.

  8. 面部软组织损伤的整形美容修复分析%Analysis of Plastic Surgery for Repairing Facial Soft Tissue Injury

    Institute of Scientific and Technical Information of China (English)

    牛兴红

    2014-01-01

    目的:探讨整形美容修复面部软组织损伤的效果。方法:选取本院整形科门诊与住院部在2012年3月~2013年1月间收治的100例面部软组织创伤患者,进行整形美容修复,观察治疗后效果。结果:经过修复治疗后,伤口在Ⅰ期的愈合率为90%。结论:整形美容修复对面部创面进行修复,能够有效的达到较佳的修复效果。%Objective:To study the effect of cosmetic surgery to repair facial soft tissue injuries. Methods:Selecting 100 patients with facial soft tissue trauma in plastic outpatient and inpatient deparments from March,2012 to January,doing cosmetic surgery repair,and observing the therapeutic effect after treatment. Results:After repair therapy,the wound in Ⅰ healing rate was 90%. Conclusion:Cosmetic surgery on facial wounds repair can effectively achieve the best effect.

  9. The Effects of Xiangqing Anodyne Spray on Treating Acute Soft-Tissue Injury Mainly Depend on Suppressing Activations of AKT and p38 Pathways

    Science.gov (United States)

    Wang, Shudong; Li, Tao; Qu, Wei; Li, Xin; Ma, Shaoxin; Wang, Zheng; Liu, Wenya; Hou, Shanshan; Fu, Jihua

    2016-01-01

    Objectives. In the present study we try to elucidate the mechanism of Xiangqing anodyne spray (XQAS) effects on acute soft-tissue injury (STI). Methods. Acute STI model was established by hammer blow in the rat hind leg muscle. Within 8 hours, instantly after modeling and per 2-hour interval repeated topical applications with or without XQAS, CP or IH ethanol extracts spray (CPS and IHS) were performed, respectively; muscle swelling rate and inflammation-related biochemical parameters, muscle histological observation, and mRNA and protein expression were then examined. Results. XQAS dose-dependently suppressed STI-caused muscle swelling, proinflammatory mediator productions, and oxidative stress as well as severe pathological changes in the injured muscle tissue. Moreover, CPS mainly by blocking p38 activation while IHS majorly by blocking AKT activation led to cytoplastic IκBα degradation with NF-κB p65 translocated into the nucleus. There are synergistic effects between CP and IH components in the XQAS on preventing from acute STI with suppressing IκBα degradation, NF-κB p65 translocation, and subsequent inflammation and oxidative stress-related abnormality. Conclusion. Marked effects of XQAS on treating acute STI are ascribed to strong anti-inflammatory and antioxidative actions with a reasonable combination of CP active components, blocking p38-NF-κB pathway activated, and IH active components, blocking AKT-NF-κB pathway activated. PMID:27190541

  10. The Effects of Xiangqing Anodyne Spray on Treating Acute Soft-Tissue Injury Mainly Depend on Suppressing Activations of AKT and p38 Pathways

    Directory of Open Access Journals (Sweden)

    Shudong Wang

    2016-01-01

    Full Text Available Objectives. In the present study we try to elucidate the mechanism of Xiangqing anodyne spray (XQAS effects on acute soft-tissue injury (STI. Methods. Acute STI model was established by hammer blow in the rat hind leg muscle. Within 8 hours, instantly after modeling and per 2-hour interval repeated topical applications with or without XQAS, CP or IH ethanol extracts spray (CPS and IHS were performed, respectively; muscle swelling rate and inflammation-related biochemical parameters, muscle histological observation, and mRNA and protein expression were then examined. Results. XQAS dose-dependently suppressed STI-caused muscle swelling, proinflammatory mediator productions, and oxidative stress as well as severe pathological changes in the injured muscle tissue. Moreover, CPS mainly by blocking p38 activation while IHS majorly by blocking AKT activation led to cytoplastic IκBα degradation with NF-κB p65 translocated into the nucleus. There are synergistic effects between CP and IH components in the XQAS on preventing from acute STI with suppressing IκBα degradation, NF-κB p65 translocation, and subsequent inflammation and oxidative stress-related abnormality. Conclusion. Marked effects of XQAS on treating acute STI are ascribed to strong anti-inflammatory and antioxidative actions with a reasonable combination of CP active components, blocking p38-NF-κB pathway activated, and IH active components, blocking AKT-NF-κB pathway activated.

  11. Slit-Robo Signal Pathway in Tissue Injury and Repair%Slit-Robo信号传导通路与组织损伤修复

    Institute of Scientific and Technical Information of China (English)

    刘丽华

    2012-01-01

    Slit proteins are secreted ligands that interact with Roundabout (Robo) receptors and have an important role in axon guidance , axonal migration, leukocyte chemotaxis, and cell migration. Current research of the Slit-Robo signal pathway shows expression in many types of injured tissues including traumas, inflammations and tumors. This article reviews current research of the Slit-Robo signal pathway with regards to tissue injury and repair.%Slit是一种分泌型的细胞外基质蛋白,它通过与其受体Roundabout( Roundabout,Robo)结合而发挥生物活性.早期研究发现Slit-Robo是神经轴突导向分子、神经元迁移的排斥因子,对白细胞趋化也有抑制作用;近年来发现Slit-Robo信号通路还能调节全身多个系统的多种细胞迁移.随着对Slit-Robo信号通路的深入研究,发现其在各种损伤、感染及肿瘤组织及其修复过程中都有表达,且其功能不尽相同.现就其在各种异常组织中的研究做一总结和回顾.

  12. A taxonomically unique Acinetobacter strain with proteolytic and hemolytic activities recovered from a patient with a soft tissue injury.

    Science.gov (United States)

    Almuzara, Marisa; Traglia, German Matías; Krizova, Lenka; Barberis, Claudia; Montaña, Sabrina; Bakai, Romina; Tuduri, Alicia; Vay, Carlos; Nemec, Alexandr; Ramírez, María Soledad

    2015-01-01

    A taxonomically unique bacterial strain, Acinetobacter sp. A47, has been recovered from several soft tissue samples from a patient undergoing reconstructive surgery owing to a traumatic amputation. The results of 16S rRNA, rpoB, and gyrB gene comparative sequence analyses showed that A47 does not belong to any of the hitherto-known taxa and may represent an as-yet-unknown Acinetobacter species. The recognition of this novel organism contributes to our knowledge of the taxonomic complexity underlying infections caused by Acinetobacter.

  13. Decreased Tissue COX5B Expression and Mitochondrial Dysfunction during Sepsis-Induced Kidney Injury in Rats

    Science.gov (United States)

    Böhm, Lennert; Braunecker, Stefan; Adler, Christoph; De Robertis, Edoardo; Cirillo, Fabrizio

    2017-01-01

    Background. Sepsis is defined as a life-threatening organ dysfunction due to a dysregulated host response to infection. Sepsis is the dominant cause of acute kidney injury (AKI), accounting for nearly 50% of episodes of acute renal failure. Signaling cascades and pathways within the kidney are largely unknown and analysis of these molecular mechanisms may enhance knowledge on pathophysiology and possible therapeutic options. Material and Methods. 26 male Wistar rats were assigned to either a sham group (control, N = 6) or sepsis group (N = 20; cecal ligature and puncture model, 24 and 48 hours after CLP). Surviving rats (n = 12) were decapitated at 24 hours (early phase; n = 6) or 48 hours (late phase; n = 6) after CLP and kidneys removed for proteomic analysis. 2D-DIGE and DeCyder 2D software (t-test, P cytochrome c oxidase subunit B (COX5b), myosin-6 (MYH6), and myosin-7 (MYH7). A significant correlation with the proteins was found for mitochondrial energy production and electron transport. Conclusions. COX5B could be a promising biomarker candidate since a significant association was found during experimental sepsis in the present study. For future research, COX5B should be evaluated as a biomarker in both human urine and serum to identify sepsis. PMID:28246552

  14. Two-dimensional zymography differentiates gelatinase isoforms in stimulated microglial cells and in brain tissues of acute brain injuries.

    Science.gov (United States)

    Chen, Shanyan; Meng, Fanjun; Chen, Zhenzhou; Tomlinson, Brittany N; Wesley, Jennifer M; Sun, Grace Y; Whaley-Connell, Adam T; Sowers, James R; Cui, Jiankun; Gu, Zezong

    2015-01-01

    Excessive activation of gelatinases (MMP-2/-9) is a key cause of detrimental outcomes in neurodegenerative diseases. A single-dimension zymography has been widely used to determine gelatinase expression and activity, but this method is inadequate in resolving complex enzyme isoforms, because gelatinase expression and activity could be modified at transcriptional and posttranslational levels. In this study, we investigated gelatinase isoforms under in vitro and in vivo conditions using two-dimensional (2D) gelatin zymography electrophoresis, a protocol allowing separation of proteins based on isoelectric points (pI) and molecular weights. We observed organomercuric chemical 4-aminophenylmercuric acetate-induced activation of MMP-2 isoforms with variant pI values in the conditioned medium of human fibrosarcoma HT1080 cells. Studies with murine BV-2 microglial cells indicated a series of proform MMP-9 spots separated by variant pI values due to stimulation with lipopolysaccharide (LPS). The MMP-9 pI values were shifted after treatment with alkaline phosphatase, suggesting presence of phosphorylated isoforms due to the proinflammatory stimulation. Similar MMP-9 isoforms with variant pI values in the same molecular weight were also found in mouse brains after ischemic and traumatic brain injuries. In contrast, there was no detectable pI differentiation of MMP-9 in the brains of chronic Zucker obese rats. These results demonstrated effective use of 2D zymography to separate modified MMP isoforms with variant pI values and to detect posttranslational modifications under different pathological conditions.

  15. Intervention of plastic surgery for emergency facial soft tissue injury%急诊面部软组织创伤的整形外科干预

    Institute of Scientific and Technical Information of China (English)

    孙秀锋; 郑妍丽; 姜涛; 王洪燕

    2011-01-01

    [目的]探讨应用整形外科原则和技术,处理急诊面部软组织创伤的方法和经验.[方法]选择急诊面部软组织创伤病例320例,采用整形外科原则和技术行清创及Ⅰ期缝合,观察治疗效果.[结果]伤口Ⅰ期愈合,无明显瘢痕增生,无功能障碍,不需要Ⅱ期修复,美容效果良好.[结论]应用整形外科基本原则和技术处理面部急性软组织创伤,可获得满意的美容效果,值得推广应用.%[Objective] To explore the clinical method of treating emergency facial soft tissue trauma with plastic surgical principles and techniques. [ Methods] The emergency facial soft tissue injuries of 320 patients were selected. They had been debrided and repaired primarily with plastic surgical principles and techniques. Healing effects were observed. [ Results] All cases were primary healing. There were no obvious hyperplasy scars and functional disturbance. Second stage reparation was not required. The cosmetic effect was perfect. [ Conclusion] It is a satisfactory and effective method to treat emergency facial soft tissue trauma with plastic surgical principles and techniques,which can be recommended to all the surgeons.

  16. Morinda citrifolia L. (noni) and memantine attenuate periventricular tissue injury of the fourth ventricle in hydrocephalic rabbits.

    Science.gov (United States)

    Köktürk, Sibel; Ceylan, Süreyya; Etus, Volkan; Yasa, Nezih; Ceylan, Savaş

    2013-03-25

    This study was designed to evaluate the neuroprotective effects of Morinda citrifolia L. (Rubiaceae), commonly known as noni, and memantine (a N-methy-D-aspartate receptor inhibitor) on hydrocephalus-induced neurodegenerative disorders. Kaolin was injected into the cistern magna of male adult New Zealand rabbits to establish a hydrocephalus animal model. Memantine (20 mg/kg, intraperitoneally; memantine-treated group) or noni (5 mL/kg, intragastrically; noni-treated group) was administered daily for 2 weeks. Microtubule-associated protein-2 and caspase-3 immunohistochemistry were performed to detect neuronal degeneration and apoptosis in the periventricular tissue of the fourth ventricle of rabbits. Microtubule-associated protein-2 staining density was significantly decreased in the hydrocephalic group, while the staining density was significantly increased in the memantine- and noni-treated groups, especially in the noni-treated group. Noni treatment decreased the number of caspase-3-positive cells in rabbits with hydrocephalus, while memantine had no effect. These findings suggest that noni exhibits more obvious inhibitory effects on hydrocephalus-induced neurodegenerative disorders than memantine in periventricular tissue of the fourth ventricle.

  17. Morinda citrifolia L.(noni) and memantine attenuate periventricular tissue injury of the fourth ventricle in hydrocephalic rabbits

    Institute of Scientific and Technical Information of China (English)

    Sibel K(o)ktürk; Süreyya Ceylan; Volkan Etus; Nezih Yasa; Sava(s) Ceylan

    2013-01-01

    This study was designed to evaluate the neuroprotective effects of Morinda citrifolia L. (Rubiaceae), commonly known as noni, and memantine (a N-methy-D-aspartate receptor inhibitor) on hydrocephalus-induced neurodegenerative disorders. Kaolin was injected into the cistern magna of male adult New Zealand rabbits to establish a hydrocephalus animal model. Memantine (20 mg/kg, intraperitoneally; memantine-treated group) or noni (5 mL/kg, intragastrically; noni-treated group) was administered daily for 2 weeks. Microtubule-associated protein-2 and caspase-3 immunohistochemistry were performed to detect neuronal degeneration and apoptosis in the periventricular tissue of the fourth ventricle of rabbits. Microtubule-associated protein-2 staining density was significantly decreased in the hydrocephalic group, while the staining density was significantly increased in the memantine- and noni-treated groups, especially in the noni-treated group. Noni treatment decreased the number of caspase-3-positive cells in rabbits with hydrocephalus, while memantine had no effect. These findings suggest that noni exhibits more obvious inhibitory effects on hydrocephalus-induced neurodegenerative disorders than memantine in periventricular tissue of the fourth ventricle.

  18. Local soft tissue musculoskeletal disorders and injuries. The role of physical and rehabilitation medicine physicians. The European perspective based on the best evidence. A paper by the UEMS-PRM Section Professional Practice Committee.

    Science.gov (United States)

    Oral, A; Ilieva, E M; Küçükdeveci, A A; Varela, E; Valero, R; Berteanu, M; Christodoulou, N

    2013-10-01

    One of the objectives of the Professional Practice Committee (PPC) of the Physical and Rehabilitation Medicine (PRM) Section of the Union of European Medical Specialists (UEMS) is the development of the field of competence of PRM physicians in Europe. To achieve this objective, UEMS PRM Section PPC has adopted a systematic action plan of preparing a series of papers describing the role of PRM physicians in a number of disabling health conditions, based on the evidence of effectiveness of PRM interventions. Soft tissue musculoskeletal disorders (MSDs) and injuries are associated with significant pain and loss of function that may lead to significant disability. The aim of this paper is to define the role of PRM physician in the management of local soft tissue MSDs and injuries with their specific focus on assessing and improving function as well as participation in the community. The training of PRM specialists make them well equipped to successfully treat MSDs including soft tissue MSDs and injuries. PRM specialists may well meet the needs of patients with soft tissue MSDs and injuries using PRM approaches including 1) assessment based on the comprehensive model of functioning, the International Classification of Functioning, Disability and Health (ICF), that enable them to identify the areas of impaired functioning in order to apply necessary measures; 2) accurate diagnosis using instrumental diagnostic procedures in addition to clinical examination; 3) outcome measurements available to them; 4) evidence-based pharmacological and nonpharmacological treatments; and finally 5) maintenance of social involvement including "return to work" based on restoration of function, all of which will eventually result in improved quality of life for patients with soft tissue MSDs and injuries.

  19. 软组织损伤患者采用冷热疗法治疗的护理分析%Analysis of patient care and soft tissue injury treatment using hot and cold therapy

    Institute of Scientific and Technical Information of China (English)

    徐杰; 刘洋; 李玉璞

    2015-01-01

    目的:探讨软组织损伤患者冷热疗法治疗和护理效果。方法选择2010年1月至2012年12月之间收治的100例软组织损伤患者为观察对象,所有患者均接受冷热疗法治疗,回顾分析不同类型软组织损伤患者临床治疗效果。结果冷热疗法治疗后,各种类型软组织损伤患者临床症状均显著改善,治疗效果较为理想。结论软组织损伤患者接受冷热疗法治疗及相应的护理,具有较为满意的临床效果。%Objective To investigate the nursing effect of hot and cold therapy on soft tissue injury patients. Meth-ods 100 cases of soft tissue injury from January 2010 to December 2012 in our hospital were selected,all patients were treated with hot and cold therapy,clinical outcomes of different types of soft tissue injuries were retrospective a-nalysis. Results After hot and cold therapy,the clinical symptoms of various soft tissue injury were significantly im-proved and therapeutic effect is better. Conclusion With hot and cold therapy and appropriate care,patients with soft tissue injuries could receive satisfactory clinical results.

  20. 冷热疗法治疗软组织损伤的护理分析%Analysis of patient care and soft tissue injury treatment using hot and cold therapy

    Institute of Scientific and Technical Information of China (English)

    徐杰

    2015-01-01

    ObjectiveTo investigate the soft tissue injury patients and nursing effect of hot and cold therapy.Methods The study selected 100 cases of medical soft tissue injury in patients treated between January 2010 to December 2012 in our hospital for the observed object, all patients were treated with hot and cold therapy, retrospective analysis of clinical outcomes in patients with different types of soft tissue injuries.Results After cold therapy, the clinical symptoms of various types of soft tissue injury were significantly improved therapeutic effect is better.Conclusion The findings of medical studies show that patients with soft-tissue injuries to receive hot and cold therapy and appropriate care, with satisfactory clinical results.%目的:探讨软组织损伤患者采用冷热疗法治疗的护理效果。方法本次医学研究选择我院2010年1月~2012年12月收治的100例软组织损伤患者作为观察对象,所有患者均接受冷热疗法治疗,回顾分析不同类型软组织损伤患者的疗效。结果冷热疗法治疗后,各种类型软组织损伤患者临床症状均显著改善,疗效较为理想。结论本次医学研究结果表明,软组织损伤患者接受冷热疗法治疗及相应的护理,具有较为满意的疗效。

  1. Kidney injury secondary to connective tissue disease%结缔组织疾病肾损害

    Institute of Scientific and Technical Information of China (English)

    王金泉; 季大玺

    2011-01-01

    结缔组织疾病常累及肾脏,其中继发于系统性红斑狼疮的狼疮性肾炎和继发于系统性小血管炎的血管炎性肾损害在临床上比较常见,且多数病情重、进展快,需要积极的诱导治疗和长期维持治疗.在这两方面,近年国内外研究均取得了明显的进展.文章介绍狼疮性肾炎和血管炎性肾损害的最新研究进展及南京军区南京总医院全军肾脏病研究所对这两类疾病治疗的体会.%Connective tissue diseases often involve the kidney. Among the connective tissue disease, lupus nephritis and systemic small-vessel vasculitis-mediated renal lesions are the most often seen in clinic. The condition of most patients with lupus nephritis and small-vessel vasculitis related renal lesions are serious and progress rapidly;they need intensive inducing immunosuppressive therapy and long-term maintenance therapy. Much progress has been made in this field in recent years. The recent researches about lupus nephritis and small-vessel vasculitis-related renal lesions are reviewed in this article. The experience on treatment of lupus nephritis and smallvessel vasculitis-related renal lesions in our institude is also introduced.

  2. Urinary Tissue Inhibitor of Metalloproteinase-2 (TIMP-2 • Insulin-Like Growth Factor-Binding Protein 7 (IGFBP7 Predicts Adverse Outcome in Pediatric Acute Kidney Injury.

    Directory of Open Access Journals (Sweden)

    Jens H Westhoff

    Full Text Available The G1 cell cycle inhibitors tissue inhibitor of metalloproteinase-2 (TIMP-2 and insulin-like growth factor-binding protein 7 (IGFBP7 have been identified as promising biomarkers for the prediction of adverse outcomes including renal replacement therapy (RRT and mortality in critically ill adult patients who develop acute kidney injury (AKI. However, the prognostic value of urinary TIMP-2 and IGFBP7 in neonatal and pediatric AKI for adverse outcome has not been investigated yet.The product of the urinary concentration of TIMP-2 and IGFBP7 ([TIMP-2]•[IGFBP7] was assessed by a commercially available immunoassay (NephroCheck™ in a prospective cohort study in 133 subjects aged 0-18 years including 46 patients with established AKI according to pRIFLE criteria, 27 patients without AKI (non-AKI group I and 60 apparently healthy neonates and children (non-AKI group II. AKI etiologies were: dehydration/hypovolemia (n = 7, hemodynamic instability (n = 7, perinatal asphyxia (n = 9, septic shock (n = 7, typical hemolytic-uremic syndrome (HUS; n = 5, interstitial nephritis (n = 5, vasculitis (n = 4, nephrotoxic injury (n = 1 and renal vein thrombosis (n = 1.When AKI patients were classified into pRIFLE criteria, 6/46 (13% patients fulfilled the criteria for the category "Risk", 13/46 (28% for "Injury", 26/46 (57% for "Failure" and 1/46 (2% for "Loss". Patients in the "Failure" stage had a median 3.7-fold higher urinary [TIMP-2]•[IGFBP7] compared to non-AKI subjects (P<0.001. When analyzed for AKI etiology, highest [TIMP-2]•[IGFBP7] values were found in patients with septic shock (P<0.001 vs. non-AKI I+II. Receiver operating characteristic (ROC curve analyses in the AKI group revealed good performance of [TIMP-2]•[IGFBP7] in predicting 30-day (area under the curve (AUC 0.79; 95% CI, 0.61-0.97 and 3-month mortality (AUC 0.84; 95% CI, 0.67-0.99 and moderate performance in predicting RRT (AUC 0.67; 95% CI, 0.50-0.84.This study shows that urinary [TIMP

  3. Extracellular BCL2 proteins are danger-associated molecular patterns that reduce tissue damage in murine models of ischemia-reperfusion injury.

    Directory of Open Access Journals (Sweden)

    Akiko Iwata

    Full Text Available BACKGROUND: Ischemia-reperfusion (I/R injury contributes to organ dysfunction in a variety of clinical disorders, including myocardial infarction, stroke, organ transplantation, and hemorrhagic shock. Recent investigations have demonstrated that apoptosis as an important mechanism of cell death leading to organ dysfunction following I/R. Intracellular danger-associated molecular patterns (DAMPs released during cell death can activate cytoprotective responses by engaging receptors of the innate immune system. METHODOLOGY/PRINCIPAL FINDINGS: Ischemia was induced in the mouse hind limb by tourniquet or in the heart by coronary artery ligation. Reperfusion injury of skeletal or cardiac muscle was markedly reduced by intraperitoneal or subcutaneous injection of recombinant human (rhBCL2 protein or rhBCL2-related protein A1 (BCL2A1 (50 ng/g given prior to ischemia or at the time of reperfusion. The cytoprotective activity of extracellular rhBCL2 or rhBCL2A1 protein was mapped to the BH4 domain, as treatment with a mutant BCL2 protein lacking the BH4 domain was not protective, whereas peptides derived from the BH4 domain of BCL2 or the BH4-like domain of BCL2A1 were. Protection by extracellular rhBCL2 or rhBCL2A1 was associated with a reduction in apoptosis in skeletal and cardiac muscle following I/R, concomitant with increased expression of endogenous mouse BCL2 (mBCL2 protein. Notably, treatment with rhBCL2A1 protein did not protect mice deficient in toll-like receptor-2 (TLR2 or the adaptor protein, myeloid differentiation factor-88 (MyD88. CONCLUSIONS/SIGNIFICANCE: Treatment with cytokine-like doses of rhBCL2 or rhBCL2A1 protein or BH4-domain peptides reduces apoptosis and tissue injury following I/R by a TLR2-MyD88-dependent mechanism. These findings establish a novel extracellular cytoprotective activity of BCL2 BH4-domain proteins as potent cytoprotective DAMPs.

  4. COLD THERAPY FOR ACUTE SOFT TISSUE INJURIES IN RATS%冷疗处理对大鼠急性软组织损伤的效果

    Institute of Scientific and Technical Information of China (English)

    石鹏; 沈若武; 季爱玉

    2011-01-01

    Objective To observe the effects of different cold therapies on acute soft tissue injury in rats and explore their mechanisms. Methods Forty newly born Wistar rats were evenly randomized to blank group(BG), control group (CG), experiment group 1 (EG1) and experiment group 2 (EG2). A rat model of acute soft tissue injury was created by hitting the inner thigh of rats 1mm above the knees with a self-made hammer. The rats in BG were not injured. The rats in CG received no treatment after injury. Those in EG1 were treated with bio-icebag (4 ℃ ) intermittently on the injured site, and those in EG2 were given continuous bio-icebag (4 ℃ ) treatment. All rats were sacrificed 48 hours later. Gross changes of the injured sites were observed, and scored based on Injury Syndrome Index (ISI). Muscle tissue was taken, HE staining done, histology changes were observed. Histological grading was based on the structure changes of muscle fibers, which included fiber arrangement, fragmentation, and swelling, the intermuscular septum widening, inflammatory cell infiltration, red blood cell exudation, muscle fiber degeneration, and muscle fiber connection. The expressions of IL-1β and TGF-β1 were detected with immunohistochemistry staining. Results Compared with the CG, the ISI and histological grading were significantly reduced in both experiment groups, while the expression of IL-1β was significantly reduced, and the expression of TGF-β1 was increased (F=35.21-127.32 ;q=4.61-41.25;P<0. 05).Compared with EG1, EG2 showed significantly lower ISI and histological grading, lower expression of IL-1β, and higher expression of TGF-β1 (q=4.23,29. 73; P<0.05). Conclusion Cold therapy for acute phase of soft tissue injury in rats can get treatment effectiveness, which is likely to reach the goal through lowering IL-1β expression and raising TGF-β1 expression. Continuous cold therapy is better than intermittent.%目的 观察不同冷疗方式对急性软组织损伤大鼠的治

  5. Toll-like receptor 4 and high-mobility group box 1 are critical mediators of tissue injury and survival in a mouse model for heatstroke.

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    Mohammed Dehbi

    Full Text Available The molecular mechanisms that initiate the inflammatory response in heatstroke and their relation with tissue injury and lethality are not fully elucidated. We examined whether endogenous ligands released by damaged/stressed cells such as high-mobility group box 1 (HMGB1 signaling through Toll-like receptor 4 (TLR4 may play a pathogenic role in heatstroke. Mutant TLR4-defective (C3H/HeJ and wild type (C3H/HeOuJ mice were subjected to heat stress in an environmental chamber pre-warmed at 43.5 °C until their core temperature reached 42.7°C, which was taken as the onset of heatstroke. The animals were then allowed to recover passively at ambient temperature. A sham-heated group served as a control. Mutant mice displayed more histological liver damage and higher mortality compared with wild type mice (73% vs. 27%, respectively, P<0.001. Compared to wild type mice, mutant mice exhibited earlier plasma release of markers of systemic inflammation such as HMGB1 (206 ± 105 vs. 63 ± 21 ng/ml; P = 0.0018 and 209 ± 100 vs. 46 ± 32 ng/ml; P<0.0001, IL-6 (144 ± 40 vs. 46 ± 20 pg/ml; P<0.001 and 184 ± 21 vs. 84 ± 54 pg/ml; P = 0.04, and IL-1β (27 ± 4 vs. 1.7 ± 2.3 pg/ml; P<0.0001 at 1 hour. Both strains of mice displayed early release of HMGB1 into the circulation upstream of IL-1β and IL-6 responses which remained elevated up to 24 h. Specific inhibition of HMGB1 activity with DNA-binding A Box (600 µg/mouse protected the mutant mice against the lethal effect of heat stress (60% A Box vs. 18% GST protein, P = 0.04. These findings suggest a protective role for the TLR4 in the host response to severe heat stress. They also suggest that HMGB1 is an early mediator of inflammation, tissue injury and lethality in heatstroke in the presence of defective TLR4 signaling.

  6. Effects of realgar on stress proteins, inflammatory mediators, and complement in brain tissue and serum of rats with inflammatory brain injury

    Institute of Scientific and Technical Information of China (English)

    Yishan Tang; Ningsheng Wang; Yinqing Zhang; Shaomei Ye; Weiping Ou

    2008-01-01

    BACKGROUND: The Chinese herbal compound realgar exerts detoxification effects as an adjuvant. It is suggested that realgar exerts detoxification via the following pathways: in the pathological state, realgar corrects the oxidative stress state by increasing stress levels, activating some endogenous protective factors and antagonizing the excessive release of inflammatory factors, as well as inhibiting complement activation.OBJECTIVE: To observe the changes in stress proteins, inflammatory mediators, and complement in the brain tissue and serum of rats with inflammatory brain injury, which have been treated with thc Chinese herbal compound Angong Niuhuang, and to compare the efficacy of Angong Niuhuang with that of realgar,to verify the mechanism of action of realgar.DESIGN, TIME AND SETTING: Randomized, controlled, cytological experiment, performed in the Institute of Clinical Pharmacology, Guangzhou University of Traditional Chinese Medicine in March 2006.MATERIALS: Thirty-six healthy, male, Sprague Dawley rats received 250 U/kg Bordetella pertussis via the common carotid artery within 15 seconds to induce inflammatory brain injury. Reagents and kits were as follows: Realgar and Angong Niuhuang powder (Foshan Second Pharmaceutical Factory, China), Bordetella pertussis diagnostic antigen (National Institute for the Control of Pharmaceutical and Biological Products,China), heat shock protein 70 (HSP70) enzyme-labeled immunosorbent assay (ELISA) kit (Stressgen, USA),tumor necrosis factor-α (TNF-α) ELISA kit (Biosource, USA), nitric oxide synthase (NOS) kit,Coomassie brilliant blue protein kit (Nanjing Jiancheng Bioengineering Co.,Ltd., China), and complements C3 and C4 (Shanghai Kehua Dongling Diagnositic Products Co.,Ltd., China),METHODS: Thirty-six rats were randomly and evenly divided into the following six groups: normal control,model, high-, middle-, and low-dose realgar-treated, and Angong Niuhuang-treated groups. At one hour prior to establishing the model

  7. High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD

    Directory of Open Access Journals (Sweden)

    Alberto J. León

    2009-01-01

    Full Text Available Intestinal alterations in IBD are triggered and maintained by an overexpression of proinflammatory cytokines. Additionally, increased immune activation has been found in the adjacent intestinal areas without displaying any apparent histological alterations, however, the regulatory environment is not well established. Biopsy specimens from patients with ulcerative colitis (UC and Crohn's disease (CD, from both affected and unaffected areas, and also from a group of colonic biopsies from healthy controls, were included in our study. Cytokines and markers of mucosal damage were analyzed by real-time PCR, and some of the results confirmed by western-blot and ELISA. Levels of IFNγ, TNFα, IL-6, IL-15, IL-18, and IL-23 were increased (above healthy controls in both affected and unaffected areas from IBD. IL-1β, IL-6, IL-12, and IL-27 were higher in affected areas compared to unaffected ones in UC but not CD. In general, a correlation was observed between mRNA levels of these cytokines and both iNOS and Granzyme B. SOCS-2 and SOCS-3 were also increased in the affected areas. In conclusion, the unaffected areas from IBD show increased levels of a restricted set of cytokines that may exert immune activating roles in these areas without being able to trigger tissue damage.

  8. Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice.

    Science.gov (United States)

    Nogueira, Denise Silva; Gazzinelli-Guimarães, Pedro Henrique; Barbosa, Fernando Sérgio; Resende, Nathália Maria; Silva, Caroline Cavalcanti; de Oliveira, Luciana Maria; Amorim, Chiara Cássia Oliveira; Oliveira, Fabrício Marcus Silva; Mattos, Matheus Silvério; Kraemer, Lucas Rocha; Caliari, Marcelo Vidigal; Gaze, Soraya; Bueno, Lilian Lacerda; Russo, Remo Castro; Fujiwara, Ricardo Toshio

    2016-01-01

    Ascaris spp. infection affects 800 million people worldwide, and half of the world population is currently at risk of infection. Recurrent reinfection in humans is mostly due to the simplicity of the parasite life cycle, but the impact of multiple exposures to the biology of the infection and the consequences to the host's homeostasis are poorly understood. In this context, single and multiple exposures in mice were performed in order to characterize the parasitological, histopathological, tissue functional and immunological aspects of experimental larval ascariasis. The most important findings revealed that reinfected mice presented a significant reduction of parasite burden in the lung and an increase in the cellularity in the bronchoalveolar lavage (BAL) associated with a robust granulocytic pulmonary inflammation, leading to a severe impairment of respiratory function. Moreover, the multiple exposures to Ascaris elicited an increased number of circulating inflammatory cells as well as production of higher levels of systemic cytokines, mainly IL-4, IL-5, IL-6, IL-10, IL-17A and TNF-α when compared to single-infected animals. Taken together, our results suggest the intense pulmonary inflammation associated with a polarized systemic Th2/Th17 immune response are crucial to control larval migration after multiple exposures to Ascaris.

  9. Differential protein expression in spinal cord tissue of a rabbit model of spinal cord ischemia/reperfusion injury

    Institute of Scientific and Technical Information of China (English)

    Qi Gao; Jian Dong; Jianhang Jiao; Yonghui Liang; Xiaoyu Yang; Guifeng Liu; Xiaoxue Li; Benqing Zhu; Jian Liu; Maoguang Yang; Weiwei Xia

    2012-01-01

    New Zealand rabbits were randomly divided into an ischemia group (occlusion of the abdominal aorta for 60 minutes), an ischemia-reperfusion group (occlusion of the abdominal aorta for 60 minutes followed by 48 hours of reperfusion) and a sham-surgery group. Two-dimensional gel electrophoresis detected 49 differentially expressed proteins in spinal cord tissue from the ischemia and ischemia/reperfusion groups and 23 of them were identified by mass spectrometry. In the ischemia group, the expression of eight proteins was up regulated, and that of the remaining four proteins was down regulated. In the ischemia/reperfusion group, the expression of four proteins was up regulated, and that of two proteins was down regulated. In the sham-surgery group, only one protein was detected. In the ischemia and ischemia/reperfusion groups, four proteins overlapped between groups with the same differential expression, including three that were up regulated and one down regulated. These proteins were related to energy metabolism, cell defense, inflammatory mechanism and cell signaling.

  10. 关节镜诊治关节内桡骨远端粉碎性骨折的软组织损伤%Arthroscopic assessment of soft tissue injuries in comminuted intra-articular fractures of the distal radius

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objective To diagnose and evaluate soft tissue injuries in intra-articular fractures of the distal radius using arthroscopy. Methods Twenty young patients with displaced intra-articular fractures of distal radius were recruited in this prospective study. Three AO C2 and 17 C3 fractures were included. After arthroscopic examination fractures of distal radius were treated by external fixation with limited internal fixation or open plate fixation.Results Triangular fibrocartilage complex (TFCC) injury was found in 18 patients (90%) . Most were isolated Palmer type 1D injuries (67 %). Scapho-lunate ligament injury was found in two patients: one partial tear (grade Ⅱ) and one complete tear (grade Ⅲ). Follow-ups ranged from 6 to 18 months. At the last follow-up, wrist function was excellent in one patient (6%), good in eight(44%) and fair in 11 (50%). Conclusion TFCC injuries are common in intra-articular fractures of the distal radius while injuries to scapho-luuate ligament are uncommon.

  11. Neuroendocrine and cardiac metabolic dysfunction and NLRP3 inflammasome activation in adipose tissue and pancreas following chronic spinal cord injury in the mouse

    Directory of Open Access Journals (Sweden)

    Mark S. Nash

    2013-09-01

    Full Text Available CVD (cardiovascular disease represents a leading cause of mortality in chronic SCI (spinal cord injury. Several component risk factors are observed in SCI; however, the underlying mechanisms that contribute to these risks have not been defined. Central and peripheral chronic inflammation is associated with metabolic dysfunction and CVD, including adipokine regulation of neuroendocrine and cardiac function and inflammatory processes initiated by the innate immune response. We use female C57 Bl/6 mice to examine neuroendocrine, cardiac, adipose and pancreatic signaling related to inflammation and metabolic dysfunction in response to experimentally induced chronic SCI. Using immuno-histochemical, -precipitation, and -blotting analysis, we show decreased POMC (proopiomelanocortin and increased NPY (neuropeptide-Y expression in the hypothalamic ARC (arcuate nucleus and PVN (paraventricular nucleus, 1-month post-SCI. Long-form leptin receptor (Ob-Rb, JAK2 (Janus kinase/STAT3 (signal transducer and activator of transcription 3/p38 and RhoA/ROCK (Rho-associated kinase signaling is significantly increased in the heart tissue post-SCI, and we observe the formation and activation of the NLRP3 (NOD-like receptor family, pyrin domain containing 3 inflammasome in VAT (visceral adipose tissue and pancreas post-SCI. These data demonstrate neuroendocrine signaling peptide alterations, associated with central inflammation and metabolic dysfunction post-SCI, and provide evidence for the peripheral activation of signaling mechanisms involved in cardiac, VAT and pancreatic inflammation and metabolic dysfunction post-SCI. Further understanding of biological mechanisms contributing to SCI-related inflammatory processes and metabolic dysfunction associated with CVD pathology may help to direct therapeutic and rehabilitation countermeasures.

  12. Determining the Depth of Injury in Bioengineered Tissue Models of Cornea and Conjunctiva for the Prediction of All Three Ocular GHS Categories.

    Directory of Open Access Journals (Sweden)

    Michaela Zorn-Kruppa

    Full Text Available The depth of injury (DOI is a mechanistic correlate to the ocular irritation response. Attempts to quantitatively determine the DOI in alternative tests have been limited to ex vivo animal eyes by fluorescent staining for biomarkers of cell death and viability in histological cross sections. It was the purpose of this study to assess whether DOI could also be measured by means of cell viability detected by the MTT assay using 3-dimensional (3D reconstructed models of cornea and conjunctiva. The formazan-free area of metabolically inactive cells in the tissue after topical substance application is used as the visible correlate of the DOI. Areas of metabolically active or inactive cells are quantitatively analyzed on cryosection images with ImageJ software analysis tools. By incorporating the total tissue thickness, the relative MTT-DOI (rMTT-DOI was calculated. Using the rMTT-DOI and human reconstructed cornea equivalents, we developed a prediction model based on suitable viability cut-off values. We tested 25 chemicals that cover the whole range of eye irritation potential based on the globally harmonized system of classification and labelling of chemicals (GHS. Principally, the MTT-DOI test method allows distinguishing between the cytotoxic effects of the different chemicals in accordance with all 3 GHS categories for eye irritation. Although the prediction model is slightly over-predictive with respect to non-irritants, it promises to be highly valuable to discriminate between severe irritants (Cat. 1, and mild to moderate irritants (Cat. 2. We also tested 3D conjunctiva models with the aim to specifically address conjunctiva-damaging substances. Using the MTT-DOI method in this model delivers comparable results as the cornea model, but does not add additional information. However, the MTT-DOI method using reconstructed cornea models already provided good predictability that was superior to the already existing established in vitro/ex vivo

  13. Neuroendocrine and Cardiac Metabolic Dysfunction and NLRP3 Inflammasome Activation in Adipose Tissue and Pancreas following Chronic Spinal Cord Injury in the Mouse

    Directory of Open Access Journals (Sweden)

    Gregory E. Bigford

    2013-08-01

    Full Text Available CVD (cardiovascular disease represents a leading cause of mortality in chronic SCI (spinal cord injury. Several component risk factors are observed in SCI; however, the underlying mechanisms that contribute to these risks have not been defined. Central and peripheral chronic inflammation is associated with metabolic dysfunction and CVD, including adipokine regulation of neuroendocrine and cardiac function and inflammatory processes initiated by the innate immune response. We use female C57 Bl/6 mice to examine neuroendocrine, cardiac, adipose and pancreatic signaling related to inflammation and metabolic dysfunction in response to experimentally induced chronic SCI. Using immunohistochemical, -precipitation, and -blotting analysis, we show decreased POMC (proopiomelanocortin and increased NPY (neuropeptide-Y expression in the hypothalamic ARC (arcuate nucleus and PVN (paraventricular nucleus, 1-month post-SCI. Long-form leptin receptor (Ob-Rb, JAK2 (Janus kinase/STAT3 (signal transducer and activator of transcription 3/p38 and RhoA/ROCK (Rho-associated kinase signaling is significantly increased in the heart tissue post-SCI, and we observe the formation and activation of the NLRP3 (NOD-like receptor family, pyrin domain containing 3 inflammasome in VAT (visceral adipose tissue and pancreas post-SCI. These data demonstrate neuroendocrine signaling peptide alterations, associated with central inflammation and metabolic dysfunction post-SCI, and provide evidence for the peripheral activation of signaling mechanisms involved in cardiac, VAT and pancreatic inflammation and metabolic dysfunction post-SCI. Further understanding of biological mechanisms contributing to SCI-related inflammatory processes and metabolic dysfunction associated with CVD pathology may help to direct therapeutic and rehabilitation countermeasures.

  14. CD8+ Granzyme B+–Mediated Tissue Injury vs. CD4+IFNγ+–Mediated Parasite Killing in Human Cutaneous Leishmaniasis

    Science.gov (United States)

    Santos, Claire da Silva; Boaventura, Viviane; Ribeiro Cardoso, Cristina; Tavares, Natalia; Lordelo, Morgana J; Noronha, Almério; Costa, Jackson; Borges, Valéria M.; de Oliveira, Camila I; Van Weyenbergh, Johan; Barral, Aldina; Barral-Netto, Manoel; Brodskyn, Cláudia Ida

    2013-01-01

    A protective or deleterious role of CD8+T cells in human cutaneous leishmaniasis (CL) has been debated. The present report explores the participation of CD8+T cells in disease pathogenesis as well as in parasite killing. CD8+T cells accumulated in CL lesions as suggested by a higher frequency of CD8+CD45RO+T cells and CD8+CLA+T cells compared with peripheral blood mononuclear cells. Upon Leishmania braziliensis restimulation, most of the CD8+T cells from the lesion expressed cytolytic markers, CD107a and granzyme B. Granzyme B expression in CL lesions positively correlated with lesion size and percentage of TUNEL-positive cells. We also observed a significantly higher percentage of TUNEL-positive cells and granzyme B expression in the biopsies of patients showing a more intense necrotic process. Furthermore, coculture of infected macrophages and CD8+T lymphocytes resulted in the release of granzyme B, and the use of granzyme B inhibitor, as well as z-VAD, Fas:Fc, or anti-IFN-γ, had no effect upon parasite killing. However, coculture of infected macrophages with CD4+T cells strongly increased parasite killing, which was completely reversed by anti-IFN-γ. Our results reveal a dichotomy in human CL: CD8+ granzyme B+T cells mediate tissue injury, whereas CD4+IFN-γ+T cells mediate parasite killing. PMID:23321919

  15. RBC-coupled tPA prevents cerebrovasodilatory impairment and tissue injury in pediatric cerebral hypoxia/ischemia through inhibition of ERK MAPK unregulation

    Energy Technology Data Exchange (ETDEWEB)

    Ganguly, Kumkum [Los Alamos National Laboratory; Armstead, William M [U PENNSYLVANIA; Kiessling, J W [U PENNSYLVANIA; Chen, Xiao - Han [U PENNSYLVANIA; Smith, Douglas H [U PENNSYLVANA; Higazi, Abd Ar [U PENNSYLVANIA; Cines, Douglas B [U PENNSYLVANIA; Bdeir, Khalil [U PENNSYLVANIA; Zaitsev, Sergei [U PENNSYLVANIA; Muzykantov, Vladimir R [U PENNSYLVANIA

    2008-01-01

    Babies experience hypoxia (H) and ischemia (I) from stroke. The only approved treatment for stroke is fibrinolytic therapy with tissue-type plasminogen activator (tPA). However, tPA potentiates H/I-induced impairment of responses to cerebrovasodilators such as hypercapnia and hypotension, and blockade of tPA-mediated vasoactivity prevents this deleterious effect. Coupling tPA to RBCs reduces its CNS toxicity through spatially confining the drug to the vasculature. Mitogen activated protein kinase (MAPK), a family of at least 3 kinases, is upregulated after H/I. In this study we determined if RBC-tPA given before or after cerebral H/I would preserve responses to cerebrovasodilators and prevent neuronal injury mediated through the ERK MAPK pathway. Animals given RBC-tPA maintained responses to cerebrovasodilators at levels equivalent to pre-H/I values. CSF and brain parenchymal ERK MAPK was elevated by H/I and this upregulation was potentiated by tPA, but blunted by RBC-tPA. U 0126, an ERK MAPK antagonist, also maintained cerebrovasodilation post H/I. Neuronal degeneration in CA1 hippocampus and parietal cortex after H/I was exacerbated by tPA, but ameliorated by RBC-tPA and U 0126. These data suggest that coupling tPA to RBCs may offer a novel approach towards increasing the benefit/risk ratio of thrombolytic therapy for CNS disorders associated with H/I.

  16. Association of Free Radicals and the Tissue Renin-Angiotensin System: Prospective Effects of Rhodiola, a Genus of Chinese Herb, on Hypoxia-Induced Pancreatic Injury

    Directory of Open Access Journals (Sweden)

    Ip SP

    2001-01-01

    Full Text Available The renin-angiotensin system has long been recognized as crucial factor in the regulation of the systemic blood pressure and renal electrolyte homeostasis. Numerous studies have demonstrated the presence of a local renin-angiotensin system in a variety of organs. A recent study of the pancreatic renin-angiotensin system showed that chronic hypoxia significantly increased the mRNA expression for angiotensinogen II receptor subtypes AT1b and AT2. The activation of the renin-angiotensin system may play an important role in cellular pathophysiological processes. Angiotensin II enhances the formation of reactive oxygen species via the activation of xanthine oxidase or NAD(PH oxidase. The reactive oxygen species can cause oxidative damage in the pancreas and other tissues either directly or indirectly via the formation of other radicals such as reactive nitrogen species. Rhodiola therapy may protect hypoxia-induced pancreatic injury in two ways. It prevents hypoxia-induced biological changes by increasing intracellular oxygen diffusion and efficiency of oxygen utilization. Alternatively, it reduces hypoxia-induced oxidative damage by its antioxidant activities. Additional experimental data are required to fully elucidate the mode of action of this herbal drug.

  17. 螺旋CT显示膝关节软组织损伤技术探讨%Spiral CT showed the knee joint of soft tissue injury technology discussed in this paper

    Institute of Scientific and Technical Information of China (English)

    秦妍滨; 于明

    2016-01-01

    目的:研究分析螺旋C T技术对于膝关节软组织损伤的诊断效果。方法:选取我院近年收治的36例膝关节软组织损伤患者作为研究对象,所有患者在入院后均通过了CT检查,收集所有患者的CT检查资料,以及CT检查结果,分析应螺旋CT对膝关节软组织损伤检查的操作方法以及诊断效果。结果:应用螺旋CT技术能清晰地显示患者膝关节各类骨折、关节腔、部分韧带以及软组织损伤,对膝关节软组织损伤具有较高的临床诊断价值。结论:螺旋CT联合多种重建技术对膝关节软组织损伤患者具有较高的临床诊断价值,值得在临床上广泛地推广应用。%Objective To study the analysis of the spiral CT technology to the diagnosis effect of knee joint of soft tissue injury. Methods Selection of 36 patients with knee joint soft tissue injury were admitted in our hospital in recent years as the research object, all of the patients in the hospital after all through the CT examination, all patients with CT examination information collection, and CT examination results, the analysis should be spiral CT examination to knee joint of soft tissue injury operation method and the effect of diagnosis.Results Spiral CT can clearly show that patients with knee joint fracture, articular cavity, part of the ligaments and soft tissue injury, the clinical diagnostic value of knee joint of soft tissue injury with high.Conclusion Spiral CT in combination with a variety of technical reconstruction in patients with knee joint soft tissue injury has high clinical diagnostic value, is worth popularizing widely in clinic application.

  18. 颌面部软组织损伤合并腮腺损伤的治疗体会%Maxillofacial soft tissue injuries combined treatment experience of parotid gland damage

    Institute of Scientific and Technical Information of China (English)

    陈虎

    2014-01-01

    目的:分析颌面部软组织损伤合并腮腺损伤的治疗体会。方法回顾我院收治的颌面部软组织损伤合并腮腺损伤53例患者的临床资料,分析患者治疗措施,并总结患者疗效。结果颌面部软组织损伤合并腮腺损伤53例患者经积极有效处理均治愈,平均住院时间(13.0±4.8)天。所有患者伤口I期愈合,未发生腮瘘、腮腺导管瘘等并发症,面部神经也逐渐恢复正常。结论准确判断颌面部软组织损伤合并腮腺损伤患者损伤情况,早期细致清创,合理处理,促进面部及腮腺恢复。%Objective Analysis of maxillofacial soft tissue injuries combined treatment experience of parotid gland damage.Methods Review of our hospital and maxillofacial soft tissue injuries combined clinical data of 53 patients with parotid gland damage, analysis of patient treatment, and summarizes the patient curative effect.Results 53 patients with maxillofacial soft tissue injuries combined parotid gland injury were cured by the positive and effective processing, the average length of hospital stay (13.0 ± 4.8) days. All patients with stage I wound healing, no complications such as gills ifstula, parotid duct ifstula, facial nerve also gradually returned to normal.Conclusion Accurate judgment of maxillofacial soft tissue injuries combined injury in patients with parotid gland injury, early debridement and reasonable treatment, promote facial and parotid gland.

  19. 面部软组织损伤的美容修复%Cosmetic repair of skin and soft tissue injuries on face

    Institute of Scientific and Technical Information of China (English)

    张鲜英; 刘毅; 宋玫; 刘萍

    2011-01-01

    Objective To observe the cosmetic repair method to treat skin and soft tissue injury on face post-traumatic. Methods Traumatic wounds were repaired with plastic surgery and minimal invasion technologies. From January 2006 to December 2010, 2170 patients were treated emergency in our department. Among them, 162 patients with soft injury on face were included, and they occupied 7.47% in emergency patients. Results 138 cases were repaired cosmetically on first stage.and 24 patients would be given surgical treatment on the second stage. 86 patients were followed up.the results showed that there were no significant scar .displacement and deformation. The satisfactory results appeared in all cases. Conclusion It might be helpful to reduce the incidence of secondary deformities anddisplacement of facial organs when the wounds on face are cosmetically repaired with plastic surgery and minimalinvasion technology.%目的:探讨面部软组织外伤后美容修复的方法与治疗效果.方法:以整形外科技术为基础,微创为原则,修复创伤伤口.2006年1月~2010年12月,我科急诊入院2170例,其中面部软组织损伤162人,占急诊人数的7.47%.结果:一期修复者1 38例,二期整形修复者24例.随访86例,手术后术区无明显瘢痕增生,面部各器官无移位、变形,效果良好.结论:应用整形外科技术与微创技术修复面部软组织损伤,有助于减少面部器官移位和继发畸形的发生,达到美容修复的目的.

  20. Neck Injuries and Disorders

    Science.gov (United States)

    ... or upper arms. Muscle strain or tension often causes neck pain. The problem is usually overuse, such as from ... or accidents, including car accidents, are another common cause of neck pain. Whiplash, a soft tissue injury to the neck, ...

  1. Novel roles for metallothionein-I + II (MT-I + II) in defense responses, neurogenesis, and tissue restoration after traumatic brain injury: insights from global gene expression profiling in wild-type and MT-I + II knockout mice.

    Science.gov (United States)

    Penkowa, Milena; Cáceres, Mario; Borup, Rehannah; Nielsen, Finn Cilius; Poulsen, Christian Bjørn; Quintana, Albert; Molinero, Amalia; Carrasco, Javier; Florit, Sergi; Giralt, Mercedes; Hidalgo, Juan

    2006-11-15

    Traumatic injury to the brain is one of the leading causes of injury-related death or disability, especially among young people. Inflammatory processes and oxidative stress likely underlie much of the damage elicited by injury, but the full repertoire of responses involved is not well known. A genomic approach, such as the use of microarrays, provides much insight in this regard, especially if combined with the use of gene-targeted animals. We report here the results of one of these studies comparing wild-type and metallothionein-I + II knockout mice subjected to a cryolesion of the somatosensorial cortex and killed at 0, 1, 4, 8, and 16 days postlesion (dpl) using Affymetrix genechips/oligonucleotide arrays interrogating approximately 10,000 different murine genes (MG_U74Av2). Hierarchical clustering analysis of these genes readily shows an orderly pattern of gene responses at specific times consistent with the processes involved in the initial tissue injury and later regeneration of the parenchyma, as well as a prominent effect of MT-I + II deficiency. The results thoroughly confirmed the importance of the antioxidant proteins MT-I + II in the response of the brain to injury and opened new avenues that were confirmed by immunohistochemistry. Data in KO, MT-I-overexpressing, and MT-II-injected mice strongly suggest a role of these proteins in postlesional activation of neural stem cells.

  2. Reduced inflammatory cell recruitment and tissue damage in spinal cord injury by acellular spinal cord scaffold seeded with mesenchymal stem cells.

    Science.gov (United States)

    Wang, Yu-Hai; Chen, Jian; Zhou, Jing; Nong, Feng; Lv, Jin-Han; Liu, Jia

    2017-01-01

    Therapy using acellular spinal cord (ASC) scaffolds seeded with bone marrow stromal cells (BMSCs) has previously been shown to restore function of the damaged spinal cord and improve functional recovery in a rat model of acute hemisected spinal cord injury (SCI). The aim of the present study was to determine whether BMSCs and ASC scaffolds promote the functional recovery of the damaged spinal cord in a rat SCI model through regulation of apoptosis and immune responses. Whether this strategy regulates secondary inflammation, which is characterized by the infiltration of immune cells and inflammatory mediators to the lesion site, in SCI repair was investigated. Basso, Beattie, and Bresnahan scores revealed that treatment with BMSCs seeded into an ASC scaffold led to a significant improvement in motor function recovery compared with treatment with an ASC scaffold alone or untreated controls at 2 and 8 weeks after surgery (Pscaffold significantly decreased the number of terminal deoxynucleotidyl transferase dUTP nick end labeling-positive cells, as compared with the ASC scaffold only and control groups. These results suggested that the use of BMSCs decreased the apoptosis of neural cells and thereby limited tissue damage at the lesion site. Notably, the use of BMSCs with an ASC scaffold also decreased the recruitment of macrophages (microglia; Pscaffold only groups. BMSCs regulated inflammatory cell recruitment to promote functional recovery. However, there was no significant difference in IgM-positive expression among the three groups (P>0.05). The results of this study demonstrated that BMSCs seeded into ASC scaffolds for repair of spinal cord hemisection defects promoted functional recovery through the early regulation of inflammatory cell recruitment with inhibition of apoptosis and secondary inflammation.

  3. Analysis on Clinical Effect of Mirena in Treatment of Adenomyosis%曼月乐节育环治疗子宫腺肌症临床疗效分析

    Institute of Scientific and Technical Information of China (English)

    刘桃

    2014-01-01

    Objective To explore intrauterine device on Mirena in the treatment of adenomyosis clinical efficacy and side effects .Methods 38 cases of adenomyosis treated with levonorgestrel-releasing intrauterine device were analyzed for menstrual blood volume ,degree of dysmenorrhea,and volume of uterus before and after the im-plantation of intrauterine device.Results The grades of both postoperative dysmenorrhea and menstrual volume markedly decreased,with statistical significance(P<0. 01).The uterus presented a corresponding shrinkage in volume,while there was no statistical differences.Conclusion Levonorgestrel intrauterine device has preferably curative effect and fewer side effect in the treatment of adenomyosis .%目的:探讨宫内节育器曼月乐环在治疗子宫腺肌病中的临床疗效及副反应。方法观察38例子宫腺肌症患者在放置曼月乐环前后月经量、痛经程度以及子宫体积的变化。结果子宫腺肌病患者在放置曼月乐环后痛经程度显著减轻(P<0.01),月经量显著减少(P<0.01),子宫体积相应减小(但差异无显著性)。结论曼月乐环对于治疗子宫腺肌病有较好疗效及较小的副作用。

  4. 足跟部软组织剥脱伤合并跟骨骨折的手术治疗%Surgery treatment of heel soft tissue denudation injury associated with calcaneal fractures

    Institute of Scientific and Technical Information of China (English)

    黄恩铭

    2015-01-01

    Objective To investigate the treatment methods and clinical efficacy of heel soft tissue denudation injury associated with calcaneal fractures.Methods 16 cases of heel soft tissue denudation injury associated with calcaneal fractures were undergone surgery.Firstly,a thorough debridement were applied,secondly calcaneal fractures were treated with open reduction,plate fixation and bone graft complex with antibiotics.Results All soft tissue denudation injury flap survived,while skin necrosis occurred in two cases,but wounds healed by short-term of changing dress-ing.All patients were followed up from 6 months to 24 months,no chronic soft tissue infections and osteomyelitis. Fracture healing time was 15 ~22 weeks.The last follow-up for AOFAS ankle-hind foot functional scores:excellent in 6 cases,good in 8,fair in 1.Conclusions For the heel soft tissue denudation injury associated with calcaneal frac-tures,thorough firstly debridement can provide good soft tissue conditions for the second phase fracture fixation, which can achieve satisfactory effect.%目的:探讨足跟部软组织剥脱伤合并跟骨骨折的临床治疗方法及疗效。方法对16例足跟软组织剥脱伤合并跟骨骨折患者行一期清创、二期跟骨骨折切复钢板内固定加带抗生素人工骨植骨术。结果患者足跟部剥脱伤皮瓣均一期成活(其中有2例术后初期出现表皮部分坏死,经短期换药创面顺利愈合)。患者均获得随访,时间6~24个月。未并发慢性软组织感染、骨髓炎。骨折愈合时间为15~22周。末次随访 AOFAS踝-后足功能评分:优6例,良8例,中2例。结论足跟软组织剥脱伤合并跟骨骨折,一期清创为二期骨折内固定提供良好的软组织条件,可取得满意疗效。

  5. Common overuse injuries in the young athlete.

    Science.gov (United States)

    Pengel, K Brooke

    2014-12-01

    Pediatric overuse injury is a common complaint presenting to pediatricians. Overuse injury can affect the soft tissues or bone, and results from an imbalance between training and load to the tissues and recovery time. In the skeletally immature athlete, physeal and apophyseal tissue is particularly vulnerable to overuse resulting in different patterns of injury compared to adults. Awareness of age-dependent patterns of overuse is necessary for proper recognition, treatment, and prevention of injury. This article reviews the most common pediatric overuse injuries with emphasis on risk factors, diagnosis, and treatment. Guidelines for prevention are included, as this is the key component for successful management of overuse injury in pediatric athletes.

  6. Ataxia Telangiectasia–Mutated Gene Polymorphisms and Acute Normal Tissue Injuries in Cancer Patients After Radiation Therapy: A Systematic Review and Meta-analysis

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Lihua [Department of Radiation Oncology, The First Hospital of Jilin University, Changchun (China); Cui, Jingkun [Department of Internal Medicine, Nanling School District Hospital of Jilin University, Changchun (China); Tang, Fengjiao; Cong, Xiaofeng [Cancer Center, The First Hospital of Jilin University, Changchun (China); Han, Fujun, E-mail: fujun_han@aliyun.com [Cancer Center, The First Hospital of Jilin University, Changchun (China)

    2015-04-01

    Purpose: Studies of the association between ataxia telangiectasia–mutated (ATM) gene polymorphisms and acute radiation injuries are often small in sample size, and the results are inconsistent. We conducted the first meta-analysis to provide a systematic review of published findings. Methods and Materials: Publications were identified by searching PubMed up to April 25, 2014. Primary meta-analysis was performed for all acute radiation injuries, and subgroup meta-analyses were based on clinical endpoint. The influence of sample size and radiation injury incidence on genetic effects was estimated in sensitivity analyses. Power calculations were also conducted. Results: The meta-analysis was conducted on the ATM polymorphism rs1801516, including 5 studies with 1588 participants. For all studies, the cut-off for differentiating cases from controls was grade 2 acute radiation injuries. The primary meta-analysis showed a significant association with overall acute radiation injuries (allelic model: odds ratio = 1.33, 95% confidence interval: 1.04-1.71). Subgroup analyses detected an association between the rs1801516 polymorphism and a significant increase in urinary and lower gastrointestinal injuries and an increase in skin injury that was not statistically significant. There was no between-study heterogeneity in any meta-analyses. In the sensitivity analyses, small studies did not show larger effects than large studies. In addition, studies with high incidence of acute radiation injuries showed larger effects than studies with low incidence. Power calculations revealed that the statistical power of the primary meta-analysis was borderline, whereas there was adequate power for the subgroup analysis of studies with high incidence of acute radiation injuries. Conclusions: Our meta-analysis showed a consistency of the results from the overall and subgroup analyses. We also showed that the genetic effect of the rs1801516 polymorphism on acute radiation injuries was

  7. Lawnmower injuries in children.

    LENUS (Irish Health Repository)

    Nugent, Nora

    2012-02-03

    OBJECTIVE: Power lawnmowers can pose significant danger of injury to both the operator and the bystander, from direct contact with the rotary blades or missile injury. Our objective was to review our experience with paediatric lawnmower-associated trauma, and the safety recommendations available to operators of power lawnmowers. METHODS: The patient cohort comprised paediatric (<16 years of age) patients treated for lawnmower-associated trauma, by the plastic surgery service, between 1996 and 2003. These patients were identified retrospectively. Age at the time of injury, location and extent of bony and soft tissue injuries sustained, treatment instituted and clinical outcome were recorded. Brochures and instruction manuals of six lawnmower manufacturers were reviewed, and safety recommendations noted. RESULTS: Fifteen patients were identified. The majority of injuries occurred from direct contact with the rotary blades (93%); the remaining child sustained a burn injury. Fourteen children (93%) required operative intervention. Seven patients (46%) sustained injuries resulting in amputation, two of whom had major limb amputations. All children, except the burns patient, underwent wound debridement and received antibiotic therapy. Reconstructive methods ranged from primary closure to free tissue transfer. Many patients required multiple procedures. In all instruction manuals, instructions to keep children and pets indoors or out of the yard when mowing were found. CONCLUSIONS: Lawnmower injuries can be devastating, particularly in children. Many victims have lasting deformities as a result of their injuries. Awareness of and stringent adherence to safety precautions during use of power lawnmowers can prevent many of these accidents.

  8. Back Injuries

    Science.gov (United States)

    ... extending from your neck to your pelvis. Back injuries can result from sports injuries, work around the house or in the garden, ... back is the most common site of back injuries and back pain. Common back injuries include Sprains ...

  9. Interleukin-1α expression precedes IL-1β after ischemic brain injury and is localised to areas of focal neuronal loss and penumbral tissues

    OpenAIRE

    Luheshi Nadia M; Kovács Krisztina J; Lopez-Castejon Gloria; Brough David; Denes Adam

    2011-01-01

    Abstract Background Cerebral ischemia is a devastating condition in which the outcome is heavily influenced by inflammatory processes, which can augment primary injury caused by reduced blood supply. The cytokines interleukin-1α (IL-1α) and IL-1β are key contributors to ischemic brain injury. However, there is very little evidence that IL-1 expression occurs at the protein level early enough (within hours) to influence brain damage after stroke. In order to determine this we investigated the ...

  10. Biomechanics of whiplash injury

    Institute of Scientific and Technical Information of China (English)

    CHEN Hai-bin; King H YANG; WANG Zheng-guo

    2009-01-01

    Despite a large number of rear-end collisions on the road and a high frequency of whiplash injuries reported, the mechanism of whiplash injuries is not completely understood. One of the reasons is that the injury is not necessarily accompanied by obvious tissue damage detectable by X-ray or MRI. An extensive series of biomechanics studies, including injury epidemiology, neck kinematics,facet capsule ligament mechanics, injury mechanisms and injury criteria, were undertaken to help elucidate these whiplash injury mechanisms and gain a better understanding of cervical facet pain. These studies provide the following evidences to help explain the mechanisms of the whiplash injury: (1) Whiplash injuries are generally considered to be a soft tissue injury of the neck with symptoms such as neck pain and stiffness, shoulder weakness, dizziness, headache and memory loss, etc. (2) Based on kinematical studies on the cadaver and volunteers, there are three distinct periods that have the potential to cause injury to the neck. In the first stage, flexural deformation of the neck is observed along with a loss of cervical lordosis; in the second stage, the cervical spine assumes an S-shaped curve as the lower vertebrae begin to extend and gradually cause the upper vertebrae to extend; during the final stage, the entire neck is extended due to the extension moments at both ends. (3)The in vivo environment afforded by rodent models of injury offers particular utility for linking mechanics, nociception and behavioral outcomes. Experimental findings have examined strains across the facet joint as a mechanism of whiplash injury, and suggested a capsular strain threshold or a vertebral distraction threshold for whiplash-related injury,potentially producing neck pain. (4) Injuries to the facet capsule region of the neck are a major source of post-crash pain. There are several hypotheses on how whiplash-associated injury may occur and three of these injuries are related to strains within

  11. Biomechanics of whiplash injury.

    Science.gov (United States)

    Chen, Hai-bin; Yang, King H; Wang, Zheng-guo

    2009-10-01

    Despite a large number of rear-end collisions on the road and a high frequency of whiplash injuries reported, the mechanism of whiplash injuries is not completely understood. One of the reasons is that the injury is not necessarily accompanied by obvious tissue damage detectable by X-ray or MRI. An extensive series of biomechanics studies, including injury epidemiology, neck kinematics, facet capsule ligament mechanics, injury mechanisms and injury criteria, were undertaken to help elucidate these whiplash injury mechanisms and gain a better understanding of cervical facet pain. These studies provide the following evidences to help explain the mechanisms of the whiplash injury: (1) Whiplash injuries are generally considered to be a soft tissue injury of the neck with symptoms such as neck pain and stiffness, shoulder weakness, dizziness, headache and memory loss, etc. (2) Based on kinematical studies on the cadaver and volunteers, there are three distinct periods that have the potential to cause injury to the neck. In the first stage, flexural deformation of the neck is observed along with a loss of cervical lordosis; in the second stage, the cervical spine assumes an S-shaped curve as the lower vertebrae begin to extend and gradually cause the upper vertebrae to extend; during the final stage, the entire neck is extended due to the extension moments at both ends. (3) The in vivo environment afforded by rodent models of injury offers particular utility for linking mechanics, nociception and behavioral outcomes. Experimental findings have examined strains across the facet joint as a mechanism of whiplash injury, and suggested a capsular strain threshold or a vertebral distraction threshold for whiplash-related injury, potentially producing neck pain. (4) Injuries to the facet capsule region of the neck are a major source of post-crash pain. There are several hypotheses on how whiplash-associated injury may occur and three of these injuries are related to strains

  12. 醋酸亮丙瑞林微球治疗子宫腺肌病合并不孕的临床疗效观察%Study on leuprorelin acetate in treatment of uterine adenomyosis with infertility

    Institute of Scientific and Technical Information of China (English)

    周黎明; 郑娟; 孙亦婷; 赵雅云; 夏爱丽

    2013-01-01

    Objective To study clinical efficacy of leuprorelin acetate in treatment of uterine adenomyosis with infertility.Methods From January 1,2011 to March 31,2012,166 cycles in 166 infertile patients combined with uterine adenomyosis undergoing in vitro fertilization embryo transplant (IVF-ET) with long protocol ovum induction by leuprorelin acetate in centre of medical reproduction,ningbo women and children's hospital were studied retrospectively.In the mean time,200 cycles in 200 infertile patients with tubal factors were enrolled as control group.The volume of uterus and outcome of IVF-ET were compared and studied between two groups.Results (1) Volume of uterus:in adenomyosis group,after 2-6 cycles of injecting leuprorelin acetate (3.75 mg/28 days),the mean uterine volume was shrinked from (180 ±73) cm3 to (86 ± 67) cm3 (P < 0.05).(2) Outcome of IVF-ET:the rate of embryo implantation was 39.1% in adenomyosis group and 35.8% in control group.The rate of clinical pregnancy was 54.2% in adenomyosis group and 53.7% in control group.The rate of abortion was 4.7% in adenomyosis group and 4.2% in control group.They all did not show statistical differences (P >0.05).(3) In adenomyosis group,the rate of fertilization,two pronuclear (2PN) and superior embryo were 67.2% (319/475),60.8%(289/475) and 52.9% (162/306) in patients with failed pregnancy and 74.2% (423/570),67.7%(386/570) and 62.1% (256/412) in patients with successful pregnancy after IVF-ET,which reached significant difference (P < 0.05).Conclusion Leuprorelin acetate could improve volume of uterine adenomyosis and outcome of pregnancy in patients undergoing IVF-ET.%目的 探讨醋酸亮丙瑞林微球(其他名称:贝依)治疗子宫腺肌病合并不孕的临床疗效.方法 对2011年1月1日至2012年3月31日在宁波市妇女儿童医院生殖医学中心因子宫腺肌病合并不孕接受醋酸亮丙瑞林微球超长方案超促排卵后,行体外受

  13. 整形外科技术在面部软组织损伤急诊处理中的应用%Clinical application of plastic surgery techniques in emergency treatment of facial soft tissue injuries

    Institute of Scientific and Technical Information of China (English)

    师军涛; 秦宏伟; 王新征

    2013-01-01

    目的 探讨整形外科技术在面部软组织损伤急诊处理中应用的临床效果,以及最大限度地恢复患者面部形态及功能的整形外科方法.方法 回顾性分析2009年6月至2011年6月收治的798例面部软组织创伤患者,采用整形外科技术,根据具体伤情设计不同修复方法,严格遵守无菌无创原则,进行清创、创面修复、皮瓣修复等治疗.结果 790例患者术后Ⅰ期愈合,8例伴有轻微瘢痕,随访6个月至1年,无感染、坏死、明显瘢痕等并发症发生,面部形态及功能恢复良好.结论 在面部软组织损伤急诊处理时,尽早应用整形外科技术进行Ⅰ期修复,避免创面自然愈合后发生畸形及功能障碍,无需Ⅱ期手术整形.%Objective To evaluate the clinical application and effect of plastic surgery in emergency treatment of facial soft tissue injuries,and to explore the better plastic surgery method for facial soft tissue injuries in order to regain the patient facial morphology and function maximally.Methods The clinical data of 798 patients with facial soft tissue injuries from June 2009 to June 2011 were analyzed retrospectively.And plastic surgical techniques were applied to the early treatment of facial soft tissue injuries in this group cases,according to the size of defect and the degree of deformity of the patient,different plastic surgery treatment was chosen,such as skin flap or skin graft to repair wound surfaces.In this process,one must follow sterile noninvasive principle strictly with emphasis on the technique of plastic surgery such as entire debridement,wound healing application of skin flap and so on.Results 790 cases of facial soft tissue injuries were healed by first intention without significant complications,while 8 cases of them had mild scars.During 6 to 12 months of follow-up,neither scar,nor infections and necrosis of the wound region occurred,and the morphology and function of patients' face recovered well without the

  14. Defensive effects of fullerene-C60 dissolved in squalane against the 2,4-nonadienal-induced cell injury in human skin keratinocytes HaCaT and wrinkle formation in 3D-human skin tissue model.

    Science.gov (United States)

    Kato, Shinya; Aoshima, Hisae; Saitoh, Yasukazu; Miwa, Nobuhiko

    2010-02-01

    We dissolved fullerene-C60 in squalane (LipoFullerene; LF-SQ, C60-eq.: 500 ppm) and examined its defensive effects against 2,4-nonadienal (NDA)-induced cell injury in HaCaT keratinocytes and wrinkle formation in three dimensional (3D)-human skin tissue model. NDA is an analog of 4-hydroxynonenal, one of major causes for human body odor indicative of aging and a lipophilic cell injury factor. Cell viability (% of the control) decreased to 31.6% on treatment with NDA (40 microM), but it increased to 66.0-97.5% when LF-SQ of 1-4% (C60-eq.: 5-20 ppm) was administered for 5 hr before NDA addition. The defensive effect by LF-SQ was superior to that of "squalane" alone at the same doses. NDA-induced DNA-fragmentation in HaCaT cells was suppressed by LF-SQ administered for 5 hr before NDA treatment, and LF-SQ protected HaCaT cells against apoptosis-like cell death. LF-SQ did not appreciably defend against hydrogen peroxide, though LF-SQ effectively defended against tert-butylhydroperoxide, a type of the intermediate hydrophilicity-lipophilicity degree out of other reactive oxygen species. The scanning electron microscopy demonstrated that NDA caused wrinkles and abnormal scales on keratinocytes of 3D-human skin tissue model, and structural homogeneity of the interstratum was broken, any of which were, however, markedly suppressed with LF-SQ. Squalane alone exhibited defensive effect against the skin tissue injury to some extent, but which was inferior to LF-SQ. LF-SQ might effectively capture and scavenge lipid radicals generated inside the cell membrane, because squalane acts as a lipophilic carrier of C60. C60 dissolved in squalane can be expected to serve as a cosmeceutical ingredient for anti-wrinkle formation.

  15. Modified Qingre Tiaoxue Decoction in the Treatment of Adenomyosis for 50 Cases%加味清热调血汤治疗子宫腺肌病50例

    Institute of Scientific and Technical Information of China (English)

    刘洁; 兰翠萍; 蔡美穗; 曾英雅; 李红英; 刘黎婷

    2016-01-01

    目的:观察加味清热调血汤治疗湿热瘀阻型子宫腺肌病的临床疗效,以拓宽中医药治疗子宫腺肌症的方法。方法选择符合湿热瘀阻型子宫腺肌病诊断标准的患者共50例,给予加味清热调血汤治疗,观察其临床疗效、治疗前后主要临床症状、中医证候积分、CA125值、超声检查子宫体积及安全性监测等资料。结果50例患者中,临床控制1例,显效12例,有效35例,无效2例,总有效率为96.0%。结论加味清热调血汤治疗湿热瘀阻型子宫腺肌病的疗效较满意,可作为治疗子宫腺肌症的有效方药之一,有一定的临床意义和应用前景,值得临床进一步的研究和推广。%To observe the clinical effect of the modified Qingre Tiaoxue decoction in the treatment of adenomyosis of damp-heat obstruction so as to enlarge the methods of traditional Chinese medicine in treating this disease.Method The modified Qingre Tiaoxue decoction was prescribed to fifty cases that were typically diagnosed as the type of damp-heat obstruction adenomyosis.The clinical effect, clinical symptoms, the integral calculus of the symptoms based on traditional Chinese medicine, the rate of CA125, uterus volume by ultrasound and safety monitoring before and after the prescription were observed.Result Among the fifty cases who were prescribed to used modified Qingre Tiaoxue decoction to treat the type of damp-heat obstruction adenomyosis, one case gained clinical control, 12 cases showed effect, 35 cases were proved to have effect, while 2 cases had no effect, and the total percentage of effect was 96%.Conclusion The effect of modified Qingre Tiaoxue decoction in treating the type of damp-heat obstruction adenomyosis is rather desirable, and can be used as one of the effective prescriptions to cure adenomyosis.This method is clinically significant with its good applicable prospect and worthy of further research and popularization.

  16. 骨科弹道式冲击波配合中药熏洗治疗软组织劳损%Orthopedic ballistic shock wave and herbal fumigation therapy for treatment of overuse injury of soft tissue

    Institute of Scientific and Technical Information of China (English)

    刘波; 刘辉; 赵卫侠; 伍萨

    2011-01-01

    BACKGROUND: Orthopedic ballistic shock wave is commonly used to cure the overuse injury of soft tissues, but this therapy combined with herbal fumigation simultaneously is rarely reported.OBJECTIVE: To observe the clinical effects of the orthopedic ballistic shock wave combined with Chinese medicinal herbal fumigation on overuse injury of soft tissues.METHODS: A total of 912 cases with soft tissue overuse injury were treated with the orthopedic ballistic shock wave and Chinese medicinal herbal fumigation. The pain intensity of trigger point was assessed by visual analogue scale.RESULTS AND CONCLUSION: The intensity of pain decreased obviously after one weak treatment, but there were no significant changes of visual analogue scale in the second week. Two weeks later, the intensity of pain decreased significantly compared with before treatment (P < 0.05). Compared with tennis elbow and tenovaginitis of flexor digitorum, the visual analogue scale of calcanodynia decreased slowly (P < 0.05). The therapy of orthopedic ballistic shock wave and herbal fumigation is significantly efficient for overuse injury of soft tissues.%背景:目前采用骨科弹道式冲击波治疗软组织劳损较为普遍,但与中药熏洗配合治疗的报道甚少.目的:观察骨科弹道式冲击波配合中药熏洗治疗软组织劳损的临床疗效.方法:将912 例软组织劳损患者采用骨科弹道式冲击波配合中药熏洗治疗.采用目测类比定级法评定患者痛点受压时的疼痛强度.结果与结论:经骨科弹道式冲击波配合中药熏洗治疗1周后,患者疼痛下降明显,第2周时测类比定级法评分变化不明显.2 周以后,患者疼痛强度与治疗前比较明显下降(P < 0.05).跟痛症与网球肘、屈指肌腱腱鞘炎比较,患者疼痛强度下降缓慢(P < 0.05).结果证实,骨科弹道式冲击波配合中药熏洗治疗软组织劳损疗效显著.

  17. 急性肌肉软组织损伤后不同冷疗方式处理的组织学变化%Histological changes in muscle tissues with acute injury following cryotherapy

    Institute of Scientific and Technical Information of China (English)

    石鹏; 沈若武; 季爱玉

    2011-01-01

    BACKGROUND: Cryotherapy of acute soft tissue injury has been widely used in clinical practice.OBJECTIVE: To observe the histological changes and treatment effect of different cryotherapies on the rats' acute damage of soft tissue. METHODS: Neonatal Wistar rats were randomized to normal, model, intermittent cryotherapy and continuous cryotherapy groups. Models of acute damage of soft tissue were established in model, intermittent cryotherapy and continuous cryotherapy groups. In intermittent cryotherapy group, the injury was treated by intermittent cryotherapy with ice bag at 4 °C; in the continuous cryotherapy group, the injury was treated by continuous cryotherapy with ice bag at 4 °C; the model group was not treated. Histological changes were observed at 48 hours. Injury degree was evaluated using injury symptom index.RESULTS AND CONCLUSION: Compared with model group, the scores of injury symptom index and histology were lower, interleukin-1β expression was reduced, and transforming growth factor-β1 (TGF-β1) expression was increased in intermittent cryotherapy and continuous cryotherapy groups (P < 0.05). Compared with intermittent cryotherapy group, the scores of injury symptom index and histology were reduced (P < 0.05), interleukin-1β expression was reduced (P < 0.05), and TGF-β1 expression was increased in continuous cryotherapy group (P < 0.05). Results demonstrated that cryotherapy can cure the acute damage of soft tissue by reducing interleukin-1β expression and raising TGF-β1 expression. Continuous cryotherapy is superior over intermittent cryotherapy.%背景:冷疗处理急性软组织损伤已在临床广泛应用.目的:观察不同冷疗方式对急性软组织损伤大鼠的组织学改变及治疗效果.方法:将新生Wistar大鼠随机分成正常组、模型组、间断冷敷组及持续冷敷组,后3组建立急性软组织损伤动物模型.间断冷敷组用4 ℃生物冰袋间断冷敷于损伤部位,持续冷敷组用4 ℃生物冰

  18. Injuries in racket sports among Slovenian players.

    Science.gov (United States)

    Kondric, Miran; Matković, Branka R; Furjan-Mandić, Gordana; Hadzić, Vedran; Dervisević, Edvin

    2011-06-01

    On the sample of 83 top Slovenian athletes we have studied the frequency of injuries among table tennis, tennis and badminton players, types of injuries and severity of injuries--the latter based on data of players absences from training and/or competition processes. The most liable parts to injuries are shoulder girdle (17.27%), spine (16.55%) and ankle (15.83%), while foot (10.07%) and wrist (12.23%) are slightly less liable to injuries. The most frequent injuries in racket sports pertain to muscle tissues. According to this data, the majority of injuries occur halfway through a training session or a competition event, mostly during a competition season. The injuries primarily pertain to muscle tissues; these are followed by joint and tendon injuries. There are no differences between male and female players. Compared to other racket sports players, table tennis players suffer from fewer injuries.

  19. Soft-tissue injuries from sports activities and traffic accidents--treatment with low-level laser therapy: a multicenter double-blind placebo-controlled clinical study on 132 patients

    Science.gov (United States)

    Simunovic, Zlatko; Trobonjaca, Tatjana

    2000-06-01

    The aim of current multicenter clinical study was to assess the efficacy of low energy-level laser therapy (LLLT) in the treatment of soft tissue injuries compared to the placebo and classical phyiotherapeutic procedures. This clinical study was conducted in two centers located in Locarno, Switzerland and Opatija, Croatia. Two types of irradiation techniques were used: (1) direct, skin contact technique for treatment of trigger points where IR diode laser 830 nm continuous wave was applied; and (2) scanning technique for irradiation of larger surface area with use of Helium Neon laser 632.8 nm combined with IR diode laser 904 nm pulsed wave. Results were evaluated according to clinical parameters like: hematoma, swelling, heat, pan and loss of function. The findings were statistically analyzed via chi- square test. Results have demonstrated that the recovery process was accelerated in 85 percent of patients treated with LLLT compared to the control group of patients. The results and advantages obtained proved once again the efficacy of LLLT as a new and successful way to treat soft tissue injuries.

  20. 严重大面积软组织缺损后负压封闭引流技术的护理%Nursing of severe extensive soft tissue injuries with vacuum sealing drainage technology

    Institute of Scientific and Technical Information of China (English)

    殷素凤

    2012-01-01

    目的 探索严重大面积软组织缺损后持续负压封闭吸引技术的护理.方法 回顾分析对24例严重大面积软组织损伤患者的护理,使用持续负压封闭吸引技术,保持有效引流,做好心理护理、疼痛护理、饮食护理等.结果 所有病例均治愈,无护理并发症发生.结论 术后有效的负压封闭引流、创面的正确观察、适当的心理护理、及时的营养支持等是护理的关键环节.%Objective To explore the nursing strategies for severe extensive soft tissue injuries with vacuum sealing drainage (VSD). Methods The nursing data of 24 patients with severe extensive soft tissue injuries treated with constant VSD were retrospectively analyzed. Effective and constant drainage, psychological nursing, pain nursing and diet nursing were properly administered. Results All patients were cured without any nursing complications. Conclusion Constant vacuum sealing drainage, correct wounds observation, proper psychological nursing and in- time nutritional support after surgery are key steps of nursing.

  1. Sports Injuries

    Science.gov (United States)

    ... sometimes you can injure yourself when you play sports or exercise. Accidents, poor training practices, or improper ... can also lead to injuries. The most common sports injuries are Sprains and strains Knee injuries Swollen ...

  2. Eye Injuries

    Science.gov (United States)

    The structure of your face helps protect your eyes from injury. Still, injuries can damage your eye, sometimes severely enough that you could lose your vision. Most eye injuries are preventable. If you play sports or ...

  3. Emergency repair of the facial soft tissue injuries with plastic surgery%应用整形美容技术修复面部软组织损伤的临床效果

    Institute of Scientific and Technical Information of China (English)

    徐传岗; 陈艳; 赵静

    2015-01-01

    Objective To investigate the effect of cosmetic surgery on the facial soft tissue injuries.Methods The clinical data of 52 patients with facial soft tissue injuries from June 2013 to June 2014 were analyzed retrospectively.And plastic surgical techniques were applied to the early treatment of facial soft tissue injuries in patients,according to the size of defect and the degree of deformity of the patients,different plastic surgery treatment was chosen,such as skin flap or skin graft to repair wound surfaces.In this process,one must follow sterile noninvasive principle strictly with emphasis on the technique of plastic surgery such as entire debridement,wound healing application of skin flap and so on.Results Total 49 cases of facial soft tissue injuries were healed by first intention without significant complications,while 4 cases of them had mild scars.Absorbable suture exposition appeared in 3 patients after primary healing.Those sutures were romoved after disinfected and no sequelaes were happened.All patients were recovered with good appearances and functions to the most exent.Conclusions The treatment of facial soft tissue injuries,by applying plastic and aesthetic surgery,could reduce scar and postoperative deformity,and secondary corrective procedures can be avoided in most patients.%目的 探讨应用整形美容技术修复面部软组织损伤的方法及临床效果.方法 回顾性分析52例面部软组织创伤患者,采用整形外科技术,根据具体伤情设计不同修复方法,严格遵守无菌、无创原则,进行清创、创面修复、皮瓣修复等治疗.结果 49例患者术后Ⅰ期愈合,4例伴有轻微瘢痕,3例患者Ⅰ期愈合后出现局部皮下可吸收线头外露,予以消毒后拆除,无并发症发生.随访6~12个月,无感染、无坏死、无明显瘢痕等并发症发生.52例患者面部功能及形态均达到了最大限度的修复,患者均较满意.结论 应用整形美容技术修复面部软组织损伤,

  4. Mirena Treatment of Adenomyosis Evaluation and Analysis of Adverse Reactions%曼月乐治疗子宫腺肌病的效果评价及不良反应探析

    Institute of Scientific and Technical Information of China (English)

    李秀红

    2014-01-01

    Objective The effects of Mirena adenomyosis treatment,and adverse reactions are discussed.Methods 50 cases of uterine adenomyosis patients admitted to our hospital for the object,randomized control group,the main use of methyltestosterone tablets for treatment in the experimental group were treated using Mirena.Two groups of patients for clinical efficacy were observed and compared.Results The patients in the experimental group menstrual flow after treatment was significantly less than the control group,dysmenorrhea score lower than the control group,uterine volume less than the control group,the differences are significant,statistically significant(P<0.05).Adverse reactions include controls acne,hirsutism,changes in libido and sound thicker,the experimental group adverse reactions include vaginal spotting,ring off,breast tenderness,and amenorrhea.Conclusion Mirena adenomyosis treatment,safe and reliable,worthy of popularization and application.%目的:对曼月乐治疗子宫腺肌病的效果以及不良反应进行探讨。方法选取我院收治的50例子宫腺肌病患者为对象,随机分组,对照组主要采用甲睾酮片进行治疗,实验组采用曼月乐进行治疗。对两组患者的临床疗效进行观察和比较。结果实验组患者治疗后的月经量明显比对照组少,痛经评分比对照组低,子宫体积比对照组小,差异明显,有统计学意义(P<0.05)。对照组不良反应包括痤疮、多毛、性欲改变以及声音变粗,实验组不良反应包括阴道点滴出血、环脱落、乳房胀痛以及闭经。结论采用曼月乐对子宫腺肌病进行治疗,安全可靠,值得普及和应用。

  5. Clinical efficiency of Levonorgestrel-releasing intrauterine system in the treatment of 69 cases with adenomyosis%左炔诺孕酮宫内节育系统治疗子宫腺肌病的临床观察

    Institute of Scientific and Technical Information of China (English)

    居蓉

    2014-01-01

    Objective To evaluate the clinical efficiency of Levonorgestrel-releasing intrauterine system(LNG-IUS) in the treatment of adenomyosis. Methods Sixty-nine patients with adenomyosis were treated with LNG-IUS during March 2011 to June 2013. The menstrual quality,menstrual period,VAS and VRS score were carried out before treatment and at one,three and six months after treatment. The amount and period of menstruation were valued by test. Results Among 69 patients, menstrual quality were reduced, dysmenorrhea and chronic pelvic pain were alleviated, which were significantly different from those before treatment(P<0.05).Conclusion LNG-IUS inserting inside uterine cavity can significantly alleviate the clinical symptoms of adenomyosis and improve the patients quality.%目的:观察左炔诺孕酮宫内节育系统(LNG-IUS)治疗子宫腺肌病所致痛经、月经过多及慢性盆腔痛的临床疗效。方法选取2011年3月~2013年6月门诊确诊子宫腺肌病患者69例,排除禁忌证后给予放置左炔诺孕酮宫内节育系统治疗,随访6个月,观察患者的月经量、月经期、痛经及慢性盆腔痛的改善情况。结果与放置左炔诺孕酮宫内节育系统前相比,放置后患者月经量明显减少,痛经程度、慢性盆腔痛明显改善,差异均具有统计学意义(P<0.05)。结论左炔诺孕酮宫内节育系统可以有效缓解子宫腺肌病临床症状,提高患者生活质量。

  6. 子宫内放置曼月乐节育环治疗子宫腺肌症的临床观察%Clinical observation of levonorgestrel intrauterine system in uterus treat-ing adenomyosis

    Institute of Scientific and Technical Information of China (English)

    朱少萍; 罗小茶; 黄慧文; 林小凤; 梁金兴

    2015-01-01

    Objective To investigate the clinical efficacy of levonorgestrel intrauterine system in uterus treating adeno-myosis. Methods 84 patients with adenomyosis treated in our hospital from February 2010 to October 2014 were se-lected as research objects,and they were randomly divided into control group and study group,with 42 cases in each group.control group was treated with medicine,study group was treated with levonorgestrel intrauterine system.dysmenor-rhea improvement,uterine volume,serum CA125,hemoglobin and clinical efficacy before and after treatment were com-pared between two groups. Results The total marked rate in study group was 88.1%,while in control group,it was 69.0%with statistical difference (P<0.05),symptoms of dysmenorrheal,decreased serum CA125 in study group was better than that in control group (P<0.05). Conclusion Levonorgestrel intrauterine system placed in the uterus adenomyosis treat-ment is effective and safe,which has a high clinical value.%目的:探讨子宫内放置曼月乐节育环治疗子宫腺肌症的临床效果。方法选取本院2010年2月~2014年10月收治的84例子宫腺肌症患者作为研究对象,随机分为对照组和研究组,每组42例。对照组采用药物治疗,研究组采用曼月乐节育环进行治疗,比较两组治疗前后痛经改善情况、子宫体积、血清CA125、血红蛋白及临床疗效。结果研究组的治疗总有效率为88.1%,而对照组仅为69.0%,两组差异有统计学意义(P<0.05),且研究组的痛经症状、血清CA125改善情况优于对照组(P<0.05)。结论曼月乐节育环置于子宫内治疗子宫腺肌症的效果确切、安全性高,具有较高的临床推广价值。

  7. Clinical observation of Mirena intrauterine device in the treatment of adenomyosis%曼月乐宫内节育器治疗子宫腺肌病的疗效观察

    Institute of Scientific and Technical Information of China (English)

    邓淑珍

    2014-01-01

    目的:探讨曼月乐宫内节育器( intrauterine device, IUD)治疗子宫腺肌病的临床疗效。方法选取郴州市第一人民医院中心医院2012年4月至2013年4月期间收治的子宫腺肌病患者共80例,随机分为观察组和对照组各40例。对照组给予药物治疗,观察组给予曼月乐IUD治疗,通过比较两组患者治疗前后的痛经情况、月经状况、子宫体积进行效果评价。结果治疗后两组患者临床症状均得到明显改善(P<0.05);观察组痛经程度,月经状况、子宫体积缩小程度,均明显优于对照组,差异有统计学意义(P<0.05)。结论曼月乐IUD对于治疗子宫腺肌病有一定的临床价值。%Objective To explore the clinical effect of Mirena intrauterine device on adenomyosis. Methods 80 patients with adenomyosis from April 2012 to April 2013 in The Central Hospital of Chenzhou No. 1 People 's Hospital were chosen. All cases were randomized into observation group and control group with 40 cases in each group. Patients in control group were treated with medicine while patients in observation group were treated with Mirena intrauterine device. Dysmenorrhea, menses situation, uterine volume were compared between two groups to evaluate the effect. Results Clinical symptoms of patients in both groups were improved after treatment(P<0. 05). Improvement of dysmenorrhea, menses situation and uterine volume of patients in observation group were superior to that in control group(P<0. 05). Conclusion Mirena intrauterine device is effective in treatment of adenomyosis.

  8. Injury Statistics

    Science.gov (United States)

    ... Certification Import Safety International Recall Guidance Civil and Criminal Penalties Federal Court Orders & Decisions Research & Statistics Research & Statistics Technical Reports Injury Statistics NEISS Injury ...

  9. Expression of aquaporin 1 and 4 in lung tissue of rats with contusion injury%水通道蛋白1和4在大鼠挫伤肺组织中的表达

    Institute of Scientific and Technical Information of China (English)

    孙相华; 洪文娟; 洪志鹏; 周菊; 祝艳翠

    2014-01-01

    目的:探讨水通道蛋白(AQP)1和4在老年大鼠挫伤后肺组织中的表达。方法通过自由落体模型制作老年鼠肺挫伤模型,分别于伤后1,3和6 h处死,取右侧肺上叶组织测水含量,RT-PCR法测量AQP1和4的mRNA表达,Western印迹法检测蛋白AQP1和4的表达水平。结果伤后老年大鼠的肺组织水含量比明显增加,与假手术组相比,AQP1和4的mRNA和蛋白表达在术后逐渐升高,术后6 h有显著差异(P<0.05), AQP-1的蛋白表达量亦明显升高( P<0.05)。结论 AQP1和4在挫伤后的肺组织中表达升高,靶向调节二者的表达可能对于肺挫伤后肺水肿的治疗具有重要意义。%Objective To investigate the expression of aquaporin ( AQP) 1 and 4 in lung tissues of aged rats with contusion injury . Methods The aged rat pulmonary contusion model was made by free falling body method .The rats were killed after injury for 1, 3 and 6 h. Their right upper lobe of lung tissues were took out to measure the water content .The expression of mRNA of AQP 1 and 4 were measured by PT-PCR.And the expression level of protein of AQP 1 and 4 were measured by Western blot .Results After injury, the water content in aged rats’ pulmonary tissues increased significantly .Compared with the control group , the expression of mRNA and protein of AQP 1 and 4 increased gradually after operation.And after 6 h, the differences showed statistical significance (P<0.05), as well the expression level of protein of AQP1.Conclusions The expression of AQP4 and 1 in contused lung tissue could increase significantly .Targeted adjustment might have important significance for the treatment of pulmonary edema after lung contusion .

  10. 半月板运动性损伤的组织工程修复:国际研究趋势与未来%Tissue-engineered repair of exercise-induced meniscal injury: International research trends and future

    Institute of Scientific and Technical Information of China (English)

    杨小宁

    2012-01-01

    BACKGROUND: The depth study of disciplines, such as tissue engineering, genetic engineering, biomechanics and materials science, provides a new approach for the treatment of meniscal injuries.OBJECTIVE: To multivariately analyze the literatures on the tissue-engineered repair of exercise-induced meniscal injury through Scopus database and to summarize the application progress of tissue-engineered repair of exercise-induced meniscal injury.METHODS: A retrieval was performed for the literature of the tissue-engineered repair of exercise-induced meniscal injury, using the key words of "meniscus, sports injuries, tissue engineering, repair" between January 2002 and December 2011 in Scopus database. The retrieval results were analyzed, and the trends were described in words and graphics.RESULTS AND CONCLUSION: A total of 245 literatures on the issue-engineered repair of exercise-induced meniscal injury were retrieved in Scopus database, in which most of paper were published as original articles. Eight articles were identified as classic literatures. The overall number of literature had an upward trend from 2002 to 2011. United States and England published more literature than other countries, which play an important role in the field of tissue-engineered repair of exercise-induced meniscal injury. Most of literatures were published from Rice University, University of Pennsylvania, and Radboud University Nijmegen Medical Centre. Biomaterials published most papers in this field (n=15, 6.12%). This paper provides a valuable reference for researchers to understand the overview and present situation of this field.%背景:骨组织工程学、基因工程、生物力学以及材料学等学科研究的深入,为半月板损伤的治疗提供了新途径.目的:利用Scopus数据库文献检索和深度分析功能,对于半月板运动损伤的组织工程修复的文献资料趋势进行多层次探讨分析,总结半月板运动损伤的组织工程修复的应用进展.

  11. Assessment of brain tissue injury after moderate hypothermia in neonates with hypoxic–ischaemic encephalopathy: a nested substudy of a randomised controlled trial

    OpenAIRE

    Rutherford, Mary; Ramenghi, Luca A; Edwards, A. David; Brocklehurst, Peter; Halliday, Henry; Levene, Malcolm; Strohm, Brenda; Thoresen, Marianne; Whitelaw, Andrew; Azzopardi, Denis

    2010-01-01

    Summary Background Moderate hypothermia in neonates with hypoxic–ischaemic encephalopathy might improve survival and neurological outcomes at up to 18 months of age, although complete neurological assessment at this age is difficult. To ascertain more precisely the effect of therapeutic hypothermia on neonatal cerebral injury, we assessed cerebral lesions on MRI scans of infants who participated in the Total Body Hypothermia for Neonatal Encephalopathy (TOBY) trial. Methods In the TOBY trial ...

  12. Interleukin-1α expression precedes IL-1β after ischemic brain injury and is localised to areas of focal neuronal loss and penumbral tissues

    Directory of Open Access Journals (Sweden)

    Luheshi Nadia M

    2011-12-01

    Full Text Available Abstract Background Cerebral ischemia is a devastating condition in which the outcome is heavily influenced by inflammatory processes, which can augment primary injury caused by reduced blood supply. The cytokines interleukin-1α (IL-1α and IL-1β are key contributors to ischemic brain injury. However, there is very little evidence that IL-1 expression occurs at the protein level early enough (within hours to influence brain damage after stroke. In order to determine this we investigated the temporal and spatial profiles of IL-1α and IL-1β expression after cerebral ischemia. Findings We report here that in mice, as early as 4 h after reperfusion following ischemia induced by occlusion of the middle cerebral artery, IL-1α, but not IL-1β, is expressed by microglia-like cells in the ischemic hemisphere, which parallels an upregulation of IL-1α mRNA. 24 h after ischemia IL-1α expression is closely associated with areas of focal blood brain barrier breakdown and neuronal death, mostly near the penumbra surrounding the infarct. The sub-cellular distribution of IL-1α in injured areas is not uniform suggesting that it is regulated. Conclusions The early expression of IL-1α in areas of focal neuronal injury suggests that it is the major form of IL-1 contributing to inflammation early after cerebral ischemia. This adds to the growing body of evidence that IL-1α is a key mediator of the sterile inflammatory response.

  13. 超12小时面部软组织创伤Ⅰ期修复临床观察%clinical observation of repairing primarily for over 12-houred facial soft tissue injury

    Institute of Scientific and Technical Information of China (English)

    孙秀锋; 王洪燕; 郑妍丽; 姜涛

    2012-01-01

    目的:探讨Ⅰ期整形修复超12小时面部软组织创伤的方法和经验.方法:选择我院急诊面部软组织创伤超过12h病例17例,采用积极扩创和整形外科技术Ⅰ期修复,观察治疗效果.结果:伤口均甲级愈合,无明显瘢痕增生,无功能障碍,不需要Ⅱ期修复,美容效果良好.结论:超12h面部软组织创伤,采用积极扩创和整形缝合,可获Ⅰ期修复和满意的美容效果,值得临床应用和深入研究.%Objective To explore the clinical method and experience of repairing over 12-houred emergency facial soft tissue trauma primarily with plastic surgical techniques. Methods The over 12-houred emergency facial soft tissue injuries of 17 patients were selected. They had been expanded debrided and repaired primarily with plastic surgical techniques. Healing effects were observed and compared. Results All cases were primary healing.There were no obvious scar formative or function problems.Second stage reconstruction was not needed, the cosmetic effect was perfect. Conclusion The facial soft tissue injury of over 12-hours can require primary healing and- satisfactory cosmetic result with active expanded debride and plastic surgical techniques. It is worth clinical applying and deeply researching.

  14. Laminin chain expression suggests that laminin-10 is a major isoform in the mouse hippocampus and is degraded by the tissue plasminogen activator/plasmin protease cascade during excitotoxic injury.

    Science.gov (United States)

    Indyk, J A; Chen, Z L; Tsirka, S E; Strickland, S

    2003-01-01

    Laminins are important components of the extracellular matrix, and participate in neuronal development, survival and regeneration. The tissue plasminogen activator/plasmin extracellular protease cascade and downstream laminin degradation are implicated in excitotoxin-induced neuronal degeneration. To determine which specific laminin chains are involved, we investigated the expression of laminins in the hippocampus, and the cell types expressing them. Reverse transcription-PCR demonstrated that the messenger RNAs for all laminin chains could be detected in the hippocampus. To determine the localization of laminin chain expression, immunostaining was used. This method showed that alpha5, beta1 and gamma1 are most highly expressed in the neuronal cell layers. Immunoblotting confirmed the hippocampal expression of the chains alpha5, beta1 and gamma1, and RNA in situ hybridization showed a neuronal expression pattern of alpha5, beta1 and gamma1. At early time points following intrahippocampal injection of kainate, alpha5, beta1 and gamma1 chain immunoreactivities were lost. In addition, tissue plasminogen activator-deficient mice, which are resistant to kainate-induced neuronal death, show no significant change in laminins alpha5, beta1 and gamma1 after intrahippocampal kainate injection. Taken together, these results suggest that laminin-10 (alpha5-beta1-gamma1) comprises a major neuronal laminin in the mouse hippocampus, and is degraded before neuronal death during excitotoxic injury by the tissue plasminogen activator/plasmin protease cascade. By identifying a neuronal laminin (laminin-10) that participates in neuronal degeneration after excitotoxic injury, this study clarifies the molecular definition of the extracellular matrix in the hippocampus and further defines a pathway for mechanisms of neuronal death.

  15. Caspase-1-Independent IL-1 Release Mediates Blister Formation in Autoantibody-Induced Tissue Injury through Modulation of Endothelial Adhesion Molecules

    NARCIS (Netherlands)

    Sadeghi, Hengameh; Lockmann, Anike; Hund, Anna-Carina; Samavedam, Unni K. S. R. L.; Pipi, Elena; Vafia, Katerina; Hauenschild, Eva; Kalies, Kathrin; Pas, Hendrikus; Jonkman, Marcel F.; Iwata, Hiroaki; Recke, Andreas; Schon, Michael P.; Zillikens, Detlef; Schmidt, Enno; Ludwig, Ralf J.

    2015-01-01

    Although reports documented aberrant cytokine expression in autoimmune bullous dermatoses (AIBDs), cytokine-targeting therapies have not been established in these disorders. We showed previously that IL-6 treatment protected against tissue destruction in experimental epidermolysis bullosa acquisita

  16. PROTEIN METABOLISM IN REGENERATING WOUND TISSUE: FUNCTION OF THE SULFUR AMINO ACIDS.

    Science.gov (United States)

    PROTEINS, *TISSUES(BIOLOGY), METABOLISM, TISSUES(BIOLOGY), REGENERATION(ENGINEERING), WOUNDS AND INJURIES, TISSUES(BIOLOGY), TRACER STUDIES, METHIONINE, COLLAGEN, TYROSINE, BIOSYNTHESIS, AMINO ACIDS .

  17. 左炔诺孕酮宫内缓释系统治疗子宫腺疾症的疗效分析%Curative effect analysis of levonorgestrel releasing Intrauterine system which is used in adenomyosis

    Institute of Scientific and Technical Information of China (English)

    刘侠

    2014-01-01

    Objective To study curative effect of Levonorgestrel releasing Intrauterine system which is used in the treatment of adenomyosis.Method Follow the 60 cases of adenomyosis treated with levonorgestrel-releasing intrauterine system from October,2011 to October,2012. The follow-up of two years,Before treatment and after treatment 3,6,12,24 months Visual analog scale (VAS),serum carcinoembryonic antigen (CA125) value,changes and treatment of endometrial menstrual quantity,2 years of patient satisfaction.Results The placement of levonorgestrel releasing intrauterine system after the symptoms of dysmenorrhea patients obviously relieved, menstrual quantity obvious reduction, the menstrual period is shortened obviously,ultrasound examination of the endometrial thickness was significantly thinner,Compared with before treatment, the difference had statistical significance;Compared with before treatment, no significant difference of serum CA125 level and the size of the uterus. The main adverse reactions are:vaginal spotting, a little off the ring, amenorrhea,At the end of 2 satisfaction is 94.58%,the continuation rate was 100%.Conclusion Levonorgestrel releasing intrauterine system is an effective method for treatment of uterine adenomyosis,or suitable for dysmenorrhea and menorrhagia patients with adenomyosis.%目的:探讨子宫腺疾症患者宫内放置左炔诺孕酮宫内缓释系统(LNG-IUS)的临床疗效。方法随机选取2011年10月—2012年10月因子宫腺肌症就诊于我院的患者60例,均放置左炔诺孕酮宫内缓释系统。随诊两年,观察治疗前,治疗后3,6,12和24个月时视觉模拟评分(VAS)、血清癌抗原(CA125)值,月经量、子宫内膜变化及治疗2年患者满意度。结果放置左炔诺孕酮宫内缓释系统后患者痛经症状明显缓解,月经量明显减少,经期明显缩短,超声复查子宫内膜厚度明显变薄,与治疗前比较,差异有统计学意义;血清CA125水

  18. 左炔诺孕酮宫内释放系统治疗子宫腺肌症28例临床观察%Levonorgestrel Releasing Intrauterine System in Treatment of Adenomyosis Clinical Observation of 28 Cases

    Institute of Scientific and Technical Information of China (English)

    昌新霞

    2015-01-01

    目的:探讨左炔诺孕酮宫内释放系统治疗子宫腺肌症临床观察。方法选取我院就诊的子宫腺肌症患者60例,随机分为实验组28例、对照组32例,实验组患者刮宫后按操作要求放置左炔诺孕酮宫内释放系统进行治疗,对照组患者采用米非司酮口服治疗。结果实验组患者的治愈率为32.1%、有效率为96.4%;对照组患者的治愈率21.9%、有效率为75%,两组数据差异具有统计学意义(P<0.05)。结论本文通过对左炔诺孕酮宫内释放系统治疗子宫腺肌症患者临床效果的对比研究,进一步证实了LNG-IUS为患有子宫腺肌症的患者进行临床治疗提供了新的选择方案,它不但能避孕,还使患者的月经情况得到显著改善、痛经指数大大降低、子宫体积也日渐减小,临床效果非常可观。%Objective To investigate the clinical observation of levonorgestrel releasing intrauterine system in treatment of adenomyosis. Methods 60 patients with adenomyosis in our hospital were selected,randomly divided into the experimental group of 28 cases,32 cases in the control group,atients in the experimental group after curettage according to operational equirements placed levonorgestrel releasing intrauterine system in treatment,the control group were treated by mifepristone. Results The patients in the experimental group. The cure rate was 32.1%,effective rate was 96.4%; the control group patients 21.9% cure rate,effective rate was 75%,statistical y significant differences between the two groups(P< 0.05)data. Conclusion Through comparative study on release system for the treatment of adenomyosis patients clinical effect of levonorgestrel releasing intrauterine,further confirmed the LNGIUS with adenomyosis patients provides a new selection scheme for clinical treatment,it can not only contraception,also make menstruation,dysmenorrhea patients obtained significant improvement is greatly reduced,the uterus index

  19. Application of tissue-engineered anterior cruciate ligament in repair of exercise injury%组织工程化前交叉韧带在运动损伤修复中应用

    Institute of Scientific and Technical Information of China (English)

    施海平

    2011-01-01

    背景:运动员前交叉韧带的损伤极难彻底恢复,降低运动寿命.目的:总结组织工程化前交叉韧带在运动损伤修复中的研究现状和最新进展.方法:采用计算机检索维普数据库和PubMed 数据库1994-01/2010-12 相关文章,纳入28 篇与运动性前交叉韧带损伤及组织工程韧带相关的文章,重点对组织工程前交叉韧带重建的研究进展、前交叉韧带种子细胞的来源、细胞因子在前交叉韧带重建中的应用及组织工程前交叉韧带附丽的基础研究4 个方面进行探讨.结果与结论:运动中前交叉韧带损伤后自体愈合能力极差,对于运动员伤后痊愈造成极大困难.组织工程前交叉韧带近些年来飞速发展,具有良好的应用和发展前景.但在实际应用过程中,组织工程化种子细胞的选择、支架材料的构建和组织工程前交叉韧带附丽的基础研究三者有机相互融合,并合理应用生长因子,才能在临床上起到最佳治疗效果,最终达到修复和重建前交叉韧带的目的.%BACKGROUND: Anterior cruciate ligament injury is extremely difficult for athletes to fully recover, and can shorten the exercisespan.OBJECTIVE: To summarize the recent research progress on the application of tissue-engineered anterior cruciate ligament inrepair of exercise injury.METHODS: An online search of VIP database and PubMed database was performed for relevant articles published betweenJanuary 1994 and December 2010. A total of 28 articles on sports-related anterior cruciate ligament injury and tissue-engineeredligament were retrieved. The discussion was mainly focused on the recent development of tissue-engineered anterior cruciateligament reconstruction, the sources of anterior cruciate ligament seed cells, the application of cytokine in reconstruction ofanterior cruciate ligament and the basic research on tissue-engineered anterior cruciate ligament.RESULTS AND CONCLUSION: Autohealing ability of sports

  20. Tissue-engineered nerve for repair of peripheral nerve injuries%组织工程化神经修复周围神经创伤的应用

    Institute of Scientific and Technical Information of China (English)

    傅重洋; 赵佳; 曲巍

    2013-01-01

    BACKGROUND:Recent development of bioengineering technology and tissue-engineered nerve brings a new hope for the treatment of peripheral nerve injuries, which has gradual y become a research spot. OBJECTIVE:To review the new progress in the repair of peripheral nerve injuries using seed cells, biomaterials and tissue-engineered nerve construction technology. METHODS:PubMed and CNKI were searched by the first authors for articles concerning nerve tissue engineering and repair of peripheral nerve injuries published prior to July 2013. The keywords were“tissue engineering, peripheral nerves, nerve injuries, stem cells, Schwann cells, scaffold, growth factor”in English and Chinese, respectively. The articles published recently or in the authorized journals were preferred in the same field. Final y, 63 articles were included in result analysis. RESULTS AND CONCLUSION:Up to now, there is a great advance in the tissue engineering technology for the repair of peripheral nerve injuries. However, most studies are stil in experimental step. For the clinical application of nerve tissue engineering, some problems to be solved include:(1) source and ethics of seed cells;(2) immunological rejection fol owing cellproliferation and transplantation;(3) stability and oncogenicity of transplanted cells;(4) degradation rate, optimal porosity, tube thickness and shape;(5) repair timing for in vitro tissue-engineered nerve construction;(6) local release and regulation of various neurobiological factors. With the development of science, many patients with nerve injuries can profit from the solve of these problems.%背景:近年来,随着生物工程技术以及组织工程化神经的发展给周围神经缺损的治疗带来了新的希望,已逐渐成为研究的焦点。目的:从种子细胞、生物材料以及构建周围神经组织技术3个方面综述组织工程方法修复周围神经损伤的新进展。方法:由第一作者在2013年7

  1. Clinical Treatment Experience of Tibia and Fibula Fracture Complicated With Soft Tissue Injury%胫腓骨骨折合并软组织损伤治疗临床体会

    Institute of Scientific and Technical Information of China (English)

    李蔚翔

    2015-01-01

    Objective To explore treatment method and its effect of tibia and fibula fracture complicated with soft tissue injury.Methods Choose 40 patients of tibia and ifbula fracture complicated with soft tissue injury who are treated in clinic from February 2013 to February 2014 and separate them into two groups according to different treatment methods; 20 patients in study group are given non-reamed intramedullary nail surgery treatment and 20 patients in control group are given conventional treatment; and then observe and compare treatment effects between two groups.Results Compared to control group, patients’ fracture recovery time in study group is shorter (P<0.05) with less complications (P<0.05) and treatment excellence rate is much higher (P<0.05). There is a differential between two groups.Conclusion Non-reamed intramedullary nail surgery treatment is of efifcacy in treatment of tibia and ifbula fracture complicated with soft tissue injury; thus, such a treatment is quite worthwhile to be promoted clinically.%目的:探讨胫腓骨骨折合并软组织损伤的治疗方法及效果。方法收集2013年2月~2014年2月我院胫腓骨骨折合并软组织损伤40例,按照治疗方式不同将其分成两组。实验组共20例,治疗方式选择非扩髓髓内钉治疗;对照组共20例,治疗方式为常规治疗。观察两组疗效,并比较分析。结果两组相比,实验组骨折愈合时间较短(P<0.05),并发症少(P<0.05),优良率高(P<0.05),差异显著。结论非扩髓髓内钉治疗胫腓骨骨折合并软组织损伤效果好。

  2. 左炔诺孕酮宫内缓释系统治疗子宫腺肌病疗效观察%Curative effect and observation of adenomyosis treated with levonorgestrel-releasing intrauterine device

    Institute of Scientific and Technical Information of China (English)

    杨丽晔; 崔友红; 张梅; 费华

    2011-01-01

    Objective To investigate the clinical effection of levonorgestrel-releasing intrauterine device (LNG IUS) in the treatment of adenomyosis.Methods Before LNG-IUS insertion and after 3, 6, 12, 18 months, 74 cases were asked to complete a visual analog scale ( VAS) form regarding pelvic pain, pictorial blood loss assessment chart (PBAC) form regarding menstrual blood volume and volume of uterus.Results After the 18-month visit, VAS score,PBAC score and volume of uterus were 0.74, 42.7, 206.5cm3, respectively.There were decreased significantly before LNG-IUS insertion(P<0.05).Conclusion Levonorgestrel intrauterine device is a preferable effective method in the treatment of adenomyosis.%目的 观察子宫腺肌病患者放置左炔诺孕酮宫内缓释系统(LNG-IUS;商品名:曼月乐)后的临床效果.方法 观察74例子宫腺肌病患者放置LNG-IUS前,放置后3、6、12、18个月痛经评分(VAS)、PBAC法月经量评分、子宫体积的变化.结果 痛经评分、经量评分和子宫体积大小在放置后18个月分别为0.74、42.7和206.5 cm3,与放置前相比差异有统计学意义(P<0.05).结论 曼月乐是治疗子宫腺肌病的一种较为有效且安全的方法.

  3. Visceral injuries.

    Science.gov (United States)

    Wisner, D H; Blaisdell, F W

    1992-06-01

    Abdominal visceral injuries are encountered by every surgeon who deals with trauma. It is simple and useful to divide abdominal visceral injuries into those caused by penetrating mechanisms of injury and those due to blunt mechanisms. Determination of the need for operative intervention is generally easier after penetrating trauma. Gunshot wounds to the abdomen should be explored, as should stab wounds to the anterior abdomen that penetrate the fascia. A midline incision is the standard approach to abdominal visceral injuries because of its ease and versatility. Abdominal exploration should be consistent and systemic so as not to miss significant injuries. Hollow viscus injury is most common after penetrating injury, while blunt injury most often results in injury to solid viscera. Diagnostic and operative aspects of the treatment of specific visceral injuries are reviewed.

  4. Release of Tissue-specific Proteins into Coronary Perfusate as a Model for Biomarker Discovery in Myocardial Ischemia/Reperfusion Injury

    DEFF Research Database (Denmark)

    Cordwell, Stuart; Edwards, Alistair; Liddy, Kiersten

    2012-01-01

    Diagnosis of acute coronary syndromes is based on protein biomarkers, such as the cardiac troponins (cTnI/cTnT) and creatine kinase (CK-MB) that are released into the circulation. Biomarker discovery is focused on identifying very low abundance tissue-derived analytes from within albumin-rich pla......Diagnosis of acute coronary syndromes is based on protein biomarkers, such as the cardiac troponins (cTnI/cTnT) and creatine kinase (CK-MB) that are released into the circulation. Biomarker discovery is focused on identifying very low abundance tissue-derived analytes from within albumin...

  5. 济民风湿王抗炎、镇痛和对急性软组织损伤作用%Therapeutic Effect of Jiming Fengshiwang on Anti-inflammation, Analgesic and Acute Soft Tissue Injury

    Institute of Scientific and Technical Information of China (English)

    滕忠; 李茂; 周军; 饶伟源; 覃良

    2011-01-01

    Objective:To observe the fluence of Jiming Fengshiwang on anti-inflammation, analgesic and cute soft tissue injury disease. Method: Jiming Fengshiwang were given 10. 0,5. 0, 2. 5 mL·kg-1 daily by external administration for 7 days. The anti-nflammatory effect was investigated by methods of ear swelling in mice, hind paw edema and adjuvant-induced arthritis in rats. The analgesic effect was observed with hot-plate test in mice and tail-contracting test with light-hot stimulus in mice. The effects of Jiming Fengshiwang on soft tissue injury in mice was tested by damages caused by freely falling mass damages models. Results; Jiming Fengshiwang could obviously inhibit ear swelling in mice induced by croton oil, hind paw edema in rats induced by carrageenan and the original and secondary inflammatory changes of adjuvant-induced arthritis in rats, could otviously prolong the pain threshold of temperature stimuli in mice, and resisting the acute soft tissue injury. Conclusion; Jiming Fengshiwang has anti-inflammation, analgesic, treating soft tissue injury effects.%目的:考察济民风湿王抗炎、镇痛和对急性软组织损伤作用.方法:受试药外用,连续给药7d,济民风湿王给药剂量小鼠、大鼠分别为10.0,5.0,2.5 mL·kg-1.通过小鼠耳肿胀法、大鼠足肿法、对佐剂性关节炎的影响观察其抗炎作用;采用热板法和光热致痛法观察其镇痛作用;自由落体组织损伤模型观察其抗急性软组织损伤作用.结果:济民风湿王能明显减轻巴豆油引起的小鼠耳肿,明显减轻角叉菜胶所致大鼠足肿,明显抑制佐剂性关节炎的原发和继发性炎症反应(P <0.01, P<0.05);显著提高热板试验中小鼠的痛阈值、光热瘤阈值(P <0.01,P<0.05);明显降低损伤大鼠的表观损伤积分,改善损伤组织的病理学形态(P<0.01,P<0.05).结论:济民风湿王具有抗炎镇痛,抗急性软组织损伤的作用.

  6. Efficacy Observation on Salt Packets of Chinese Medicine with Hot Compress treating Soft Tissue Injury%中药盐包热熨治疗软组织损伤的疗效观察

    Institute of Scientific and Technical Information of China (English)

    刘艺敏

    2013-01-01

    Objective: To observe the clinical curative effect on salt packets of traditional Chinese medicine with hot compress treat-ing soft tissue injury. Methods: Heated salt packets of traditional Chinese medicine were placed in the injury site, and supplemented with appropriate massage. Results: 162 cases showed effect, 44 were valid, 20 were invalid, and the total efficiency was 91.15%. Conclusion: The salt packets of traditional Chinese medicine can play a role in accelerating decrease of swelling, pain and blood sta-sis, they are well received by the patients because of obvious efficacy.%  目的:观察中药盐包热熨治疗软组织损伤的临床疗效.方法:制作中药盐包加热置于损伤部位,并辅以适当的推拿手法.结果:显效162例,有效44例,无效20例,总有效率91.15%.结论:中药盐包能发挥加快消肿、止痛、散瘀等作用,疗效显著,深受病人好评.

  7. Photobiomodulation on sports injuries

    Science.gov (United States)

    Liu, Xiao-Guang; Liu, Timon C.; Jiao, Jian-Ling; Li, Cheng-Zhang; Xu, Xiao-Yang

    2003-12-01

    Sports injuries healing has long been an important field in sports medicine. The stimulatory effects of Low intensity laser (LIL) irradiation have been investigated in several medical fields, such as cultured cell response, wound healing, hormonal or neural stimulation, pain relief and others. The aim of this study was to evaluate whether LIL irradiation can accelerate sports injuries healing. Some experimental and clinical studies have shown the laser stimulation effects on soft tissues and cartilage, however, controversy still exists regarding the role of LIL when used as a therapeutic device. Summarizing the data of cell studies and animal experiments and clinic trials by using the biological information model of photobiomodulation, we conclude that LIL irradiation is a valuable treatment for superficial and localized sports injuries and that the injuries healing effects of the therapy depend on the dosage of LIL irradiation.

  8. Exogenous ATP administration prevents ischemia/reperfusion-induced oxidative stress and tissue injury by modulation of hypoxanthine metabolic pathway in rat ovary

    Directory of Open Access Journals (Sweden)

    Serkan Kumbasar

    2014-07-01

    Full Text Available In this study, xanthine oxidase (XO, malondialdehyde (MDA, myeloperoxidase (MPO and glutathione (GSH levels in the ovarian tissues of rats during the development of ischemia and postischemia-induced reperfusion were investigated, and the effect of ATP on ischemia-reperfusion (I/R damage was biochemically and histopathologically examined. The results of the biochemical analyses demonstrated that ATP significantly reduced the level of XO and MDA and increased the amount of GSH in both ischemia and I/R-applied ovarian tissue at the doses administered. Furthermore, ATP significantly suppressed the increase in MPO activity that occurred following the application of post ischemia reperfusion in the ovarian tissue. The biochemical results obtained in the present study coincide with the histological findings. The severity of the pathological findings, such as dilatation, congestion, haemorrhage, oedema and polymorphonuclear nuclear leukocytes (PMNLs, increased in parallel with the increase observed in the products of XO metabolism. In conclusion, exogenously applied ATP prevented I/R damage by reducing the formation of XO in ischemic ovarian tissue.

  9. Dynamics of cerebral tissue injury and perfusion after temporary hypoxia-ischemia in the rat - Evidence for region-specific sensitivity and delayed damage

    NARCIS (Netherlands)

    Dijkhuizen, RM; Knollema, S; van der Worp, H. Bart; Ter Horst, GJ; De Wildt, DJ; van der Sprenkel, JWB; Tulleken, KAF; Nicolay, K

    1998-01-01

    Background and Purpose-Selective regional sensitivity and delayed damage in cerebral ischemia provide opportunities for directed and late therapy for stroke. Our aim was to characterize the spatial and temporal profile of ischemia-induced changes in cerebral perfusion and tissue status, with the use

  10. Clinical Observation on the Treatment of Soft Tissue Injury by Ultrasonic Wave%超声波治疗运动性软组织损伤临床观察

    Institute of Scientific and Technical Information of China (English)

    严芳; 庞艳丽; 张会霞; 杨小龙

    2016-01-01

    Objective To observe clinical curative effect of ultrasonic treatment of motor soft tissue injuries.Methods 56 patients were randomly divided into treatment group and control group,28 cases in each group.Treatment group by ultrasonic treatment,control group is made of super laser treatment.Observe two groups of curative effect.Results Treatment group total effectiveness 92.9%,control group total effectiveness 75%,treatment group curative effect is better than that of control group,both have significant difference ( <0.01).Conclusion Ultrasound in the treatment of various kinds of sports soft tissue injuries curative effect is distinct,is worthy of popularization and application.%目的:观察超声波治疗运动性软组织损伤临床疗效。方法将56例患者随机分为治疗组和对照组,每组28例。治疗组采用超声波治疗,对照组采用超激光治疗,观察两组疗效。结果治疗组总有效率92.9%,对照组总有效率75%,治疗组疗效优于对照组,两者比较有显著性差异(<0.01)。结论超声波在治疗各种运动型软组织损伤中疗效显著,值得推广应用。

  11. β7 Integrin controls mast cell recruitment, whereas αE integrin modulates the number and function of CD8+ T cells in immune complex-mediated tissue injury.

    Science.gov (United States)

    Yamada, Daisuke; Kadono, Takafumi; Masui, Yuri; Yanaba, Koichi; Sato, Shinichi

    2014-05-01

    Immune complex (IC) deposition causes significant tissue injury associated with various autoimmune diseases such as vasculitis. In the cascade of inflammation, cell-to-cell and cell-to-matrix adhesion via adhesion molecules are essential. To assess the role of αE and β7 integrin in IC-mediated tissue injury, peritoneal and cutaneous reverse-passive Arthus reaction was examined in mice lacking αE integrin (αE(-/-)) or β7 integrin (β7(-/-)). Both αE(-/-) and β7(-/-) mice exhibited significantly attenuated neutrophil infiltration in the peritoneal and cutaneous Arthus reaction. β7 integrin deficiency, not αE integrin deficiency, significantly reduced the number of mast cells in the peritoneal cavity, which was consistent with the result that mast cells expressed only α4β7 integrin, not αEβ7 integrin. αE(-/-) mice instead revealed the reduction of CD8(+) T cells in the peritoneal cavity, and nearly half of them in wild-type mice expressed αE integrin. These αE(+)CD8(+) T cells produced more proinflammatory cytokines than αE(-)CD8(+) T cells, and adoptive transfer of αE(+)CD8(+) T cell into αE(-/-) recipients restored cutaneous and peritoneal Arthus reaction. These results suggest that in the peritoneal and cutaneous reverse-passive Arthus reaction, α4β7 integrin is involved in the migration of mast cells for initial IC recognition. αEβ7 integrin, in contrast, contributes by recruiting αE(+)CD8(+) T cells, which produce more proinflammatory cytokines than αE(-)CD8(+) T cells and amplify IC-mediated inflammation.

  12. Tamoxifen and estradiol improved locomotor function and increased spared tissue in rats after spinal cord injury: their antioxidant effect and role of estrogen receptor alpha.

    Science.gov (United States)

    Mosquera, Laurivette; Colón, Jennifer M; Santiago, José M; Torrado, Aranza I; Meléndez, Margarita; Segarra, Annabell C; Rodríguez-Orengo, José F; Miranda, Jorge D

    2014-05-01

    17β-Estradiol is a multi-active steroid that imparts neuroprotection via diverse mechanisms of action. However, its role as a neuroprotective agent after spinal cord injury (SCI), or the involvement of the estrogen receptor-alpha (ER-α) in locomotor recovery, is still a subject of much debate. In this study, we evaluated the effects of estradiol and of Tamoxifen (an estrogen receptor mixed agonist/antagonist) on locomotor recovery following SCI. To control estradiol cyclical variability, ovariectomized female rats received empty or estradiol filled implants, prior to a moderate contusion to the spinal cord. Estradiol improved locomotor function at 7, 14, 21, and 28 days post injury (DPI), when compared to control groups (measured with the BBB open field test). This effect was ER-α mediated, because functional recovery was blocked with an ER-α antagonist. We also observed that ER-α was up-regulated after SCI. Long-term treatment (28 DPI) with estradiol and Tamoxifen reduced the extent of the lesion cavity, an effect also mediated by ER-α. The antioxidant effects of estradiol were seen acutely at 2 DPI but not at 28 DPI, and this acute effect was not receptor mediated. Rats treated with Tamoxifen recovered some locomotor activity at 21 and 28 DPI, which could be related to the antioxidant protection seen at these time points. These results show that estradiol improves functional outcome, and these protective effects are mediated by the ER-α dependent and independent-mechanisms. Tamoxifen׳s effects during late stages of SCI support the use of this drug as a long-term alternative treatment for this condition.

  13. The application of comprehensive physicotherapeutics in the facial soft tissue injury%综合物理治疗在面部软组织挫伤后的应用

    Institute of Scientific and Technical Information of China (English)

    杨青; 刘剑毅; 曹川; 李世荣

    2011-01-01

    目的 探讨综合物理治疗在促进面部软组织挫伤恢复、防治并发症等方面的作用.方法 将56例面部软组织挫伤患者分成综合物理治疗组29例和非物理治疗组27例,综合物理治疗组应用微波、等幅中频正弦电(音频)、超声波、手法按摩等物理治疗并配戴低温热塑板材成型的面部支具进行局部压迫.非物理治疗组伤后不进行任何物理治疗,待其自然恢复.结果 综合物理治疗组面部软组织挫伤处局部淤血、肿痛、感觉障碍、皮下组织粘连4周恢复率为97%,非物理治疗组伤后4周恢复率为74%,显著低于综合物理治疗组.结论 面部软组织挫伤后物理治疗联合低温热塑板材加压的综合治疗能迅速消除面部软组织挫伤后的肿痛、促进淤血吸收、减少面部软组织挫伤引起的感觉障碍、皮下组织粘连引起的面部凹陷或动力性凹陷等并发症,是面部软组织挫伤后良好的治疗手段.%Objective To explore the method of treating the facial soft tissue injury with comprehensive physicotherapeutics and preventing the complication. Methods Altogether 56 facial soft tissue injury outpatients were divided into comprehensive physico therapeutics group(29) and non-comprehensive physicotherapeutics group (27). Micrewave therapy, acoustic frequency therapy, ultrasonic therapy,maneuver massage therapy and hypothermia forming board for pression were used in the comprehensive physico therapeutics group. No therapy were used for non-comprehensive physicotherapeutics group. Results The recovery rate of local haemostasis,pain,sensation disorder and subcutaneous tissue conglutination after 30 days was 97% in the comprehensive physicotherapeutics group. However, the recovery rate of those in non-comprehensive physicotherapeutics group was 74%. Conclusion Comprehensive physicotherapeutics combined with hypothermia forming board for pression can quickly eliminate the local haemostasis, pain

  14. 坏死组织对深二度烧伤创面进行性加深的影响%Effect of necrotic tissue on progressive injury in deep partial thickness burn wounds

    Institute of Scientific and Technical Information of China (English)

    陆树良; 向军; 青春; 金曙雯; 廖镇江; 史济湘

    2002-01-01

    目的 为探讨深二度烧伤创面坏死组织持续存在对创面组织进行性损害的影响。方法 将7例施行削痂手术的深Ⅱ度烧伤患者创面分为手术前、手术后、和坏死组织存在的未手术创面,并分别获取创面组织标本,测定创面组织释放IL-8、EGF、bFGF、PDGF-AB水平以及观察组织形态学变化。结果 7例未手术创面组织在体外组织培养中释放IL-8水平较削痂手术后创面水平有明显升高(P<0.001);同时,未手术创面EGF、bFGF、PDGF-AB水平与其配对的削痂手术后创面比较均呈明显降低(P<0.05-<0.005)。局部组织形态学观察亦显示,坏死组织存在的未手术创面除有大量中性粒细胞浸润外,坏死范围较手术前扩大,残留的少量皮肤附件因炎症扩大而消失,而削痂后创面的炎性反应较未手术和手术前有明显减轻,无坏死范围的进一步扩大,甚至可见新生肉芽形成和部分表皮修复。结论 创面坏死组织持续存在不仅是创面愈合过程的抑制因素,而且还可能因炎症反应加强或持续存在而导致创面进一步加深,使创面愈合更为延迟。削痂手术作为一种去除坏死组织的主动手段,对促进创面愈合有积极意义。%Objective To evaluate the influence of necrotic tissue on progressive injury in deep partial thickness burn wounds.Methods Tissue specimens were cultured both for estimation of IL-8, EGF, bFGF, PDGF-AB and histopathological examination, from the pre-operation, post-operation, and non-operation wounds from seven patients with deep partial thickness burn.Results In seven specimens from the non-operation group, IL-8 release increased compared with those in the post-operation group (P<0.001), while the levels of EGF, bFGF, PDGF-AB release were lower than those in the post-operation group. Histopathological examination revealed that in the non-operation group, the degree of neutrophil

  15. Muscle strain injuries.

    Science.gov (United States)

    Garrett, W E

    1996-01-01

    One of the most common injuries seen in the office of the practicing physician is the muscle strain. Until recently, little data were available on the basic science and clinical application of this basic science for the treatment and prevention of muscle strains. Studies in the last 10 years represent action taken on the direction of investigation into muscle strain injuries from the laboratory and clinical fronts. Findings from the laboratory indicate that certain muscles are susceptible to strain injury (muscles that cross multiple joints or have complex architecture). These muscles have a strain threshold for both passive and active injury. Strain injury is not the result of muscle contraction alone, rather, strains are the result of excessive stretch or stretch while the muscle is being activated. When the muscle tears, the damage is localized very near the muscle-tendon junction. After injury, the muscle is weaker and at risk for further injury. The force output of the muscle returns over the following days as the muscle undertakes a predictable progression toward tissue healing. Current imaging studies have been used clinically to document the site of injury to the muscle-tendon junction. The commonly injured muscles have been described and include the hamstring, the rectus femoris, gastrocnemius, and adductor longus muscles. Injuries inconsistent with involvement of a single muscle-tendon junction proved to be at tendinous origins rather than within the muscle belly. Important information has also been provided regarding injuries with poor prognosis, which are potentially repairable surgically, including injuries to the rectus femoris muscle, the hamstring origin, and the abdominal wall. Data important to the management of common muscle injuries have been published. The risks of reinjury have been documented. The early efficacy and potential for long-term risks of nonsteroidal antiinflammatory agents have been shown. New data can also be applied to the field

  16. Head Injuries

    Science.gov (United States)

    ... ATV) Safety Balance Disorders Knowing Your Child's Medical History First Aid: Falls First Aid: Head Injuries Preventing Children's Sports Injuries Getting Help: Know the Numbers Concussions Stay Safe: Baseball Concussions Concussions: Getting Better Sports and Concussions Dealing ...

  17. Ear Injury

    Science.gov (United States)

    ... Fundamentals Heart and Blood Vessel Disorders Hormonal and Metabolic Disorders Immune Disorders Infections Injuries and Poisoning Kidney and ... Fundamentals Heart and Blood Vessel Disorders Hormonal and Metabolic Disorders Immune Disorders Infections Injuries and Poisoning Kidney and ...

  18. Genital injury

    Science.gov (United States)

    ... each side of a bar, such as a monkey bar or the middle of a bicycle Symptoms ... Names Scrotal trauma; Straddle injury; Toilet seat injury Images Female reproductive anatomy Male reproductive anatomy Normal female ...

  19. Knee Injuries

    Science.gov (United States)

    ... bursitis . Symptoms of bursitis in the knee include warmth, tenderness, swelling, and pain on the front of ... injury without the aid of a television screen. Physical Therapy Depending on the type of knee injury ...

  20. 肌肉组织工程研究现状及对运动性肌肉损伤的作用%Research status and effect of muscle tissue engineering on exercise-induced muscle injury

    Institute of Scientific and Technical Information of China (English)

    王鹏举

    2011-01-01

    fesue engineering for be treatment of muscle injuries has a good prospect.However, in practice applications, muscle tissue-engineered seed cells selection, scaffold construction, extraceHular matrixcomposite materials research and organizational compatibility are the still the difficult problems of muscle tissue engineering.

  1. Patterns of Pediatric Maxillofacial Injuries.

    Science.gov (United States)

    Bede, Salwan Yousif Hanna; Ismael, Waleed Khaleel; Al-Assaf, Dhuha

    2016-05-01

    Facial trauma in children and adolescents is reported to range from 1% to 30%. Because of many anatomical, physiological, and psychological characteristics of the pediatric population, maxillofacial injuries in children should be treated with special consideration that is attributable to certain features inherent in facial growth patterns of children. This study evaluated maxillofacial injuries in 726 children in terms of incidence, patterns of injury, causes, and treatment modalities and compared these parameters among 3 pediatric age groups. Intergroup differences were analyzed using Z test for 2 populations' proportion. The results showed that the incidence of pediatric maxillofacial injuries and fractures is higher than that reported elsewhere with male predominance. Soft tissue injuries are more frequently encountered in younger individuals, whereas the incidence of skeletal injuries increases with age. This study also revealed that certain etiologies, namely road traffic accidents, violence, bicycle, missiles, and industrial injures, increase with age; on the other hand, falls and puncture wounds are more common in younger children.

  2. Orienteering injuries

    OpenAIRE

    Folan, Jean M.

    1982-01-01

    At the Irish National Orienteering Championships in 1981 a survey of the injuries occurring over the two days of competition was carried out. Of 285 individual competitors there was a percentage injury rate of 5.26%. The article discusses the injuries and aspects of safety in orienteering.

  3. Management of Extensor Tendon Injuries

    Science.gov (United States)

    Griffin, M; Hindocha, S; Jordan, D; Saleh, M; Khan, W

    2012-01-01

    Extensor tendon injuries are very common injuries, which inappropriately treated can cause severe lasting impairment for the patient. Assessment and management of flexor tendon injuries has been widely reviewed, unlike extensor injuries. It is clear from the literature that extensor tendon repair should be undertaken immediately but the exact approach depends on the extensor zone. Zone I injuries otherwise known as mallet injuries are often closed and treated with immobilisaton and conservative management where possible. Zone II injuries are again conservatively managed with splinting. Closed Zone III or ‘boutonniere’ injuries are managed conservatively unless there is evidence of displaced avulsion fractures at the base of the middle phalanx, axial and lateral instability of the PIPJ associated with loss of active or passive extension of the joint or failed non-operative treatment. Open zone III injuries are often treated surgically unless splinting enable the tendons to come together. Zone V injuries, are human bites until proven otherwise requires primary tendon repair after irrigation. Zone VI injuries are close to the thin paratendon and thin subcutaneous tissue which strong core type sutures and then splinting should be placed in extension for 4-6 weeks. Complete lacerations to zone IV and VII involve surgical primary repair followed by 6 weeks of splinting in extension. Zone VIII require multiple figure of eight sutures to repair the muscle bellies and static immobilisation of the wrist in 45 degrees of extension. To date there is little literature documenting the quality of repairing extensor tendon injuries however loss of flexion due to extensor tendon shortening, loss of flexion and extension resulting from adhesions and weakened grip can occur after surgery. This review aims to provide a systematic examination method for assessing extensor injuries, presentation and management of all type of extensor tendon injuries as well as guidance on

  4. Recovery by N-acetylcysteine from subchronic exposure to Imidacloprid-induced hypothalamic-pituitary-adrenal (HPA) axis tissues injury in male rats.

    Science.gov (United States)

    Annabi, Alya; Dhouib, Ines Bini; Lamine, Aicha Jrad; El Golli, Nargès; Gharbi, Najoua; El Fazâa, Saloua; Lasram, Mohamed Montassar

    2015-01-01

    Imidacloprid is the most important example of the neonicotinoid insecticides known to target the nicotinic acetylcholine receptor in insects, and potentially in mammals. N-Acetyl-l-cysteine (NAC) has been shown to possess curative effects in experimental and clinical investigations. The present study was designed to evaluate the recovery effect of NAC against Imidacloprid-induced oxidative stress and cholinergic transmission alteration in hypothalamic-pituitary-adrenal (HPA) axis of male rats following subchronic exposure. About 40 mg/kg of Imidacloprid was administered daily by intragastric intubation and 28 days later, the rats were sacrificed and HPA axis tissues were removed for different analyses. Imidacloprid increased adrenal relative weight and cholesterol level indicating an adaptive stage of the general alarm reaction to stress. Moreover, Imidacloprid caused a significant increase in malondialdehyde level, the antioxidants catalase, superoxide dismutase and glutathione-S-transferase showed various alterations following administration and significant depleted thiols content was only recorded in hypothalamic tissue. Furthermore, the hypothalamic and pituitary acetylcholinesterase activity and calcium level were significantly increased highlighting the alteration of cholinergic activity. The present findings revealed that HPA axis is a sensitive target to Imidacloprid (IMI). Interestingly, the use of NAC for only 7 days post-exposure to IMI showed a partial therapeutic effect against Imidacloprid toxicity.

  5. Injuries in competitive boxing. A prospective study.

    Science.gov (United States)

    Siewe, J; Rudat, J; Zarghooni, K; Sobottke, R; Eysel, P; Herren, C; Knöll, P; Illgner, U; Michael, J

    2015-03-01

    Boxing remains a subject of controversy and is often classified as dangerous. But the discussion is based mostly on retrospective studies. This survey was conducted as a prospective study. From October 2012 to September 2013, 44 competitive boxers were asked to report their injuries once a month. The questionnaire collected general information (training, competition) and recorded the number of bouts fought, injuries and resulting lost days. A total of 192 injuries were recorded, 133 of which resulted in interruption of training or competition. Each boxer sustained 3 injuries per year on average. The injury rate was 12.8 injuries per 1 000 h of training. Boxers fighting more than 3 bouts per year sustain more injuries (p=0.0075). The injury rate does is not a function of age (age≤19 vs. > 19a, p=0.53). Injuries to the head and the upper limbs occur most frequently. The most common injuries are soft tissue lacerations and contusions. Head injuries with neurological symptoms rarely occur (4.2%). Boxing has a high injury rate that is comparable with other contact sports, but most injuries are minor. Injury frequency is not a function of whether the boxer competes in the junior or adult category. Athletes fighting many bouts per year have a greater risk of injury.

  6. 茶多酚对急性运动后大鼠骨骼肌组织损伤的保护作用%The protective effects of tea polyphenol on rats' skeletal muscle tissue injury caused by acute exercise/

    Institute of Scientific and Technical Information of China (English)

    刘霞; 李素云; 周春仙

    2011-01-01

    目的:探讨茶多酚对急性运动后大鼠骨骼肌组织损伤的保护作用.方法:30只SD大鼠分为对照组(A)、运动组(B)和运动加茶多酚组(C),建立力竭运动模型,1周后测试骨骼肌组织钙离子(Ca2+)含量、钠泵(Na+-K+-ATP酶)和钙泵(Ca2+-ATP酶)活性,以及在电镜和光镜下观察腓肠肌的形态和超微结构的变化.结果:运动组与对照组相比,骨骼肌组织Ca2+含量显著升高(P<0.05),Ca2+-ATP酶和Na+-K+-ATP酶的显著下降(P<0.05),运动加茶多酚组与运动组相比,骨骼肌Ca2+含量显著减少(P<0.05),Ca2+-ATP酶活性有上升趋势,Na+-K+-ATP酶活性显著升高(P<0.05).光镜和电镜观察运动组有明显的形态学变化和超微结构的损伤,运动加茶多酚组的超微结构损伤有明显改善.结论:茶多酚可降低急性运动后骨骼肌组织中钙离子的含量,修复骨骼肌组织的损伤.%Objective: To approach the protection effects of tea polyphenol on rats' skeletal muscle tissue injury caused by acute exercise.Method:Thirty rats were randomly divided into 3 groups, control group(A), exercise group (B) and exercise with tea polyphenol group(C). The rat model of acute exhausted exercise was established. One week later the content of Ca2+, the activities of Na+-K+-ATPase and Ca2+-ATPase in skeletal muscles were measured, and the changes of morphology and altrastructure of gastrocnemius were observed under optical and electric microscopes.Result: Compared with A group, the content of Ca2 + increased(P< 0.05),activities of Ca2 +-ATPase and Na+-K+-ATPase dropped(P<0.05) in B group. Compared with B group, the content of Ca2+ decreased(P<0.05), actrivity of Ca2+-ATPase had rising tendency and activity of Na+-K+-ATPase rose(P<0.05) in C group. Evident morphological changes and ultrastructure injury were observed in B group, but in C group ultrastructure injury was lighter than those in B group.Conclusion: Tea polyphenol can reduce the content of Ca2+ in skeleton

  7. Bicycling injuries.

    Science.gov (United States)

    Silberman, Marc R

    2013-01-01

    Bicycling injuries can be classified into bicycle contact, traumatic, and overuse injuries. Despite the popularity of cycling, there are few scientific studies regarding injuries. Epidemiological studies are difficult to compare due to different methodologies and the diverse population of cyclists studied. There are only three studies conducted on top level professionals. Ninety-four percent of professionals in 1 year have experienced at least one overuse injury. Most overuse injuries are mild with limited time off the bike. The most common site of overuse injury is the knee, and the most common site of traumatic injury is the shoulder, with the clavicle having the most common fracture. Many overuse and bicycle contact ailments are relieved with simple bike adjustments.

  8. Quantitative indicator of cortisone in human adrenal tissue as a criterion for determining the duration of the duration of fatal injury

    Directory of Open Access Journals (Sweden)

    Shkand T.V.

    2013-01-01

    Full Text Available To elucidate pathogenetic mechanisms of myocardium necrosis, to develop the preventive and treatment measures its experimental studying in different models is necessary. The paper covers the dynamics of morphological changes in myocardium after cryoeffect in heart for 15 and 30 sec, ligation of left coronary artery and introduction of adrenalin toxic doses. It has been established that cryoeffect to heart led to the appearance of necrotic zone in myocardium with no ischemic inflammation phase, herewith the depth of cardiac muscle lesion depended directly on cryoeffect duration. The ligation of coronary artery contributed to the formation of ischemic necrosis of myocardium in the zone of ligated vessel basin with manifested discirculatory disorders. Focus lesion of myocardium resulted from the introduction of toxic doses of adrenalin was focused around arterial vessels, which later led to periarterial proliferation of fibroblasts and formation of granulation tissue.

  9. Induction of VMAT-1 and TPH-1 expression induces vesicular accumulation of serotonin and protects cells and tissue from cooling/rewarming injury.

    Directory of Open Access Journals (Sweden)

    Fatemeh Talaei

    Full Text Available DDT₁ MF-2 hamster ductus deferens cells are resistant to hypothermia due to serotonin secretion from secretory vesicles and subsequent cystathionine beta synthase (CBS mediated formation of H₂S. We investigated whether the mechanism promoting resistance to hypothermia may be translationally induced in cells vulnerable to cold storage. Thus, VMAT-1 (vesicular monoamino transferase and TPH-1 (tryptophan hydroxylase were co-transfected in rat aortic smooth muscle cells (SMAC and kidney tissue to create a serotonin-vesicular phenotype (named VTSMAC and VTkidney, respectively. Effects on hypothermic damage were assessed. VTSMAC showed a vesicular phenotype and an 8-fold increase in serotonin content and 5-fold increase in its release upon cooling. Cooled VTSMAC produced up to 10 fold higher concentrations of H₂S, and were protected from hypothermia, as shown by a 50% reduction of caspase 3/7 activity and 4 times higher survival compared to SMAC. Hypothermic resistance was abolished by the inhibition of CBS activity or blockade of serotonin re-uptake. In VTkidney slices, expression of CBS was 3 fold increased in cold preserved kidney tissue, with two-fold increase in H₂S concentration. While cooling induced substantial damage to empty vector transfected kidney as shown by caspase 3/7 activity and loss of FABP1, VTkidney was fully protected and comparable to non-cooled control. Thus, transfection of VMAT-1 and TPH-1 induced vesicular storage of serotonin which is triggered release upon cooling and has protective effects against hypothermia. The vesicular serotonergic phenotype protects against hypothermic damage through re-uptake of serotonin inducing CBS mediated H₂S production both in cells and kidney slices.

  10. 左炔诺孕酮宫内缓释系统对子宫腺肌症患者卵巢功能的影响%Effect of levonorgestrel releasing intrauterine system to adenomyosis ovarian function in patients

    Institute of Scientific and Technical Information of China (English)

    朱允英

    2016-01-01

    Objective To observe the effect of levonorgestrel releasing intrauterine system to adenomyosis ovarian function in patients. Methods Patients with adenomyosis hospital from March 2014 to June 2015 were treated 70 cases, according to the chronological order of admission into two groups, control group and the observation group, 35 patients in each. The control group were treated with mifepristone treatment, the observation group were treated with levonorgestrel-releasing intrauterine system for treatment, the two groups were observed menstrual menstruation and ovarian case index level. Results The observation group patients menstrual period, menstrual cycle and dysmenorrhea degree than the control group, the difference was significant (P<0.05);observation group patients ovarian function level indicator values than the control group, the difference was significant (P<0.05). Conclusion Levonorgestrel releasing intrauterine system used in the treatment of patients with adenomyosis, ovarian function in patients with good impact and improve patient menstrual cycle and menstrual pain situation, worthy of promotion and application.%目的::观察左炔诺孕酮宫内缓释系统对子宫腺肌症患者卵巢功能的影响。方法:选取本院2014年3月~2015年6月收治的子宫腺肌症患者70例,按入院时间顺序分为两组,对照组及观察组,各35例。对照组患者给予米非司酮进行治疗,观察组患者给予左炔诺孕酮宫内缓释系统进行治疗,观察两组患者月经经期情况及卵巢指标水平。结果:观察组患者治疗后月经期、月经周期及痛经程度明显优于对照组,差异显著(P<0.05);观察组患者治疗后卵巢功能水平指标数值明显优于对照组,差异显著(P<0.05)。结论:左炔诺孕酮宫内缓释系统应用于子宫腺肌症患者治疗,对患者卵巢功能影响好,且有效改善了患者经期周期及经期疼痛情况,值得推广及应用。

  11. The Effect Observation of Levonorgestrel-releasing Intrauterine System in Adenomyosis%左炔诺孕酮宫内缓释系统治疗子宫腺肌症的临床疗效观察

    Institute of Scientific and Technical Information of China (English)

    吴小平; 林美丽

    2014-01-01

    Objective:To study clinical effect of Levonorgestrel-releasing Intrauterine System in adenomyosis.Method:40 cases of uterine adenomyosis patients in our hospital from January 2012 to March 2013 were admitted for the study,the Levonorgestrel-releasing Intrauterine System was placed into the patient utero,the menstrual flow,dysmenorrhea of the degree,uterine size change were observed and analysed before and after treatment.Result:Before treatment the dysmenorrhea score,menstrual flow,CA125,uterine size were (84.12±4.90)points,(212.01±53.01)ml,(70.38±68.50)U/ml,(190.16±90.04)cm3,placed 3 months in patients with dysmenorrhea score,menstrual flow,CA125,respectively (21.50±8.11)points,(48.21±14.88)ml,(26.01±25.42)U/ml,the difference were statistically significant(P0.05).In addition,patients had no significant adverse reactions.Conclusion:The effect of Levonorgestrel-releasing Intrauterine System in the treatment of adenomyosis is obvious,this method can effectively improve patient menstrual flow,dysmenorrhea and other symptoms,and safe,reliable,it is worthy of clinical application.%目的:观察左炔诺孕酮宫内缓释系统治疗子宫腺肌症的临床疗效。方法:选取2012年1月-2013年3月笔者所在医院收治的40例子宫腺肌症患者为研究对象,将左炔诺孕酮宫内缓释系统放置到患者宫内,对放置前后患者月经量、痛经程度、子宫大小变化等进行全面的观察和分析。结果:放置前患者痛经评分、月经量、CA125、子宫大小分别为(84.12±4.90)分、(212.01±53.01)ml、(70.38±68.50)U/ml、(190.16±90.04)cm3,放置后3个月患者痛经评分、月经量、CA125分别为(21.50±8.11)分、(48.21±14.88)ml、(26.01±25.42)U/ml,差异有统计学意义(P0.05)。此外,患者均无出现明显不良反应。结论:左炔诺孕酮宫内缓释系统治疗子宫腺肌症效果明显,能有效改善患者月经量、痛经等症状,且安全可靠,值得在临床上推广应用。

  12. Injury - kidney and ureter

    Science.gov (United States)

    Kidney damage; Toxic injury of the kidney; Kidney injury; Traumatic injury of the kidney; Fractured kidney; Inflammatory injury of the kidney; Bruised kidney; Ureteral injury; Pre-renal failure - injury, ...

  13. PPARγexpression in rat lung tissue after ventilator-induced lung injury%机械通气致大鼠肺损伤时肺组织PPARγ表达的变化

    Institute of Scientific and Technical Information of China (English)

    王秀芹; 王凯国; 罗红敏; 李浩; 王宝胜; 王培民

    2014-01-01

    Objective To observe the changes of PPARγ expression in ventilator-induced lung injury rats and explore the role of PPARγ in the pathogenesis of ventilator-induced lung injury. Methods Sixty male Sprague-Dawley rats were randomly divided into 3 groups ( n=21 each ):group N received large tidal volume with mechanical ventilation ( Vt=12 mL/kg);group C received lower tidal volume with mechanical ventilation ( Vt=6 mL/kg);group R received room air without mechanical ventilation. Rats in every group were randomly divided into 3 subgroups respectively by 1,4 and 8 h. The samples of lung were collected at 1,4 and 8 h after ventilation. Lung pathological examina-tion, total protein and white blood cells in bronchoalveolar fluid and wet-to-dry weight were detected. The exoressions of PPARγmRNA were detected by RTPCR;PPARγ protein in lung tissues was detected by western bolt. Result After 4 and 8 h ventilation in group N,total pro-tein and WBC in bronchoalvelor fluid,W/D were markedly higher than those of group C and R (P 0. 05). Conclusion PPARγmRNA and protein expressions in the rats lung tissue of ventilator-induced lung injury were decreased and as-sociated with inflammation and damage of lung tissue.%目的:观察PPARγ在通气相关性肺损伤大鼠肺组织中的表达,探讨PPARγ在通气相关性肺损伤中的作用。方法清洁雄性SD大鼠,随机分为大潮气量( Tidal volume,VT)组,Vt=12 mL;小潮气量组,Vt=6 mL;自主呼吸组。每组又分为3个亚组:即通气1h、4h、8h组。于各时间段末放血处死动物,收集肺组织和肺灌洗液标本,测定肺灌洗液中蛋白总量、白细胞计数;测定肺组织湿/干重比( W/D);逆转录聚合酶链反应检测PPARγmRNA的表达;western blot检测PPARγ蛋白的变化。结果大潮气量组机械通气4 h、8 h后,与小潮气量组及自主呼吸组相比,肺灌洗液中白细胞计数、蛋白总量明显增加(P 0.05)。结论通气相关性肺损伤大鼠肺组织

  14. 肢体软组织损伤后继发创面出血止血方法的临床分析%Personalized hemostasis in secondary wound bleeding after limb soft tissue injury

    Institute of Scientific and Technical Information of China (English)

    窦晨浩; 刘月驹; 闫晓丽; 张英泽

    2015-01-01

    Background:Although secondary vessel rupture of wound is a severe complication after trauma, there are few systematic re-ports on specific methods of hemostasis. Objective:To explore emergency and surgical hemostasis for secondary bleeding after soft tissue injuries. Methods:A retrospective investigation of 45 patients with secondary wound surface bleeding resulting from severe open soft tissue injuries treated in our hospital from January 2006 to July 2013 was conducted in this study. There were 41 males and 4 females with an average age of (39.4±15.8) years (range, 11-76 years). The interval from injury to hospitalization was (1.4± 3.2) d on average (range, 1-13 d). The injury was caused by traffic accident in 27 cases, machine hurt in 7, crush of heavy ob-ject in 2, fall in 2, electrical injury in 2, and other injuries in 5. MESS score was used to evaluate the damage of soft tissues. Results:There were 32 cases with arterial bleeding (18 with lower extremity bleeding and 14 with upper extremity bleed-ing) and 13 cases with venous bleeding (9 with lower extremity bleeding and 4 with upper extremity bleeding). There were 37 cases with MESS score≥7 and the other 8 cases with MESS score Conclusions:Hemostasis for secondary wound bleeding should be based on the following three steps. The first step is imme-diate compression hemostasis for the wound. The second step is effective hemostasis according to features, position and MESS score of the bleeding. The third step is surgical treatment.%背景:继发性创面血管破裂为创伤后的严重并发症,但对其具体止血方法却鲜有系统性报道。  目的:探讨不同软组织损伤程度、不同部位及性质出血所采取的紧急止血方法及手术止血方法。  方法:回顾性分析2006年1月至2013年7月收治的开放性软组织损伤继发创面出血患者45例。其中男41例,女4例;年龄为11~76岁,平均(39.4±15.8)岁;伤后距入院时间1~13 d

  15. Ultrasound imaging of sports-related musculoskeletal injuries

    Energy Technology Data Exchange (ETDEWEB)

    Craig, J.G.; Holsbeek, M.T. van [Div. of Musculoskeletal Radiology, Henry Ford Hospital, Detroit MI (United States); Gauthier, T.P.; Cook, W.J. [Philips Ultrasound, Bothell WA (United States)

    2006-07-01

    Sports-related injuries of the musculoskeletal system affect millions of individuals every year. Integrating high-frequency Tissue Harmonic Imaging ultrasound with MRI and CT gives the greatest opportunity for diagnosing specific injuries. (orig.)

  16. 腹腔镜下电器械对卵巢组织热损伤的临床观察%Clinical Observation of Electro-surgical Unit’s Influence on the Heat Injury of Ovarian Tissue under Laparoscopy

    Institute of Scientific and Technical Information of China (English)

    林碧娟; 林松波; 黄春玲; 颜青

    2015-01-01

    Objective:To study the electro-surgical unit’s clinical influence on the heat injury of ovarian tissue under laparoscope,for reference.Method:150 ovarian tumor patients were involved in the study,and grouped according to the random principle.They were divided into three groups:group of monopolar electrocoagulation (50 cases),group of bipolar electrocoagulation (50 cases),group of electrocoagulation ultrasonic scalpel (50 cases).The surgical procedures of three groups were compared.Result:Compared the length of surgery under Laparoscopy,length of activities,length of gas passing,length of hospital stays and the usage rate of painkiller of three groups,the differences were not statistically significant (P>0.05). Though the study of the depth and width of the zone of thermal necrosis’s heat injury of ovarian tissue,we concluded that electrocoagulation ultrasonic scalpel imposed the minimum damages,bipolar electrocoagulation the second,and monopolar electrocoagulation the maximum,the differences were statistically significant (P0.05)。从卵巢组织热损伤坏死带深度和宽度来看,超声刀组损伤最小,其次为双极电凝组,损伤最大的为单极电凝组,差异有统计学意义(P<0.05)。结论:不同电器械对卵巢组织所产生的热损伤不同,超声刀带来的热损伤最小,其次是双极电凝,单极电凝带来的热损伤最大,临床上治疗时需根据实际情况考虑,尽量选用热损伤范围小的PK刀。最好选用缝合止血,从而减少卵巢组织的损伤,保护患者术后卵巢排卵的生育功能。

  17. Tissue engineering technology and biomaterials for repair of sports articular cartilage injury%组织工程技术及生物材料修复运动性关节软骨损伤

    Institute of Scientific and Technical Information of China (English)

    马金玉; 薛媛; 杨洪武

    2011-01-01

    目的:总结组织工程技术及生物材料在防治运动性关节软骨损伤中的应用特点.方法:以"关节软骨,组织工程技术,生物材料"为中文关键词,以"tissue enginneering, articular cartilage, scaffold material"为英文关键词,采用计算机检索Pubmed数据库(http://www.ncbi.nlm.nih.gov/PubMed)及维普数据库(http://www.cqvip.com/)1993-01/2010-10的相关文章,排除重复研究或Meta分析类文章.以23篇文献为主,重点对修复运动性关节软骨损伤种子细胞、支架材料、细胞因子及其性能进行讨论.结果:计算机初检得到104篇文献,根据纳入排除标准,对组织工程软骨的种子细胞、生物支架材料以及用于组织工程中的细胞因子进行总结与分析.种子细胞是制约组织工程软骨进一步临床应用的首要因素,目前常采用的种子细胞有软骨细胞、骨髓基质干细胞和胚胎干细胞等;生物支架材料包括天然材料和人工合成可降解聚合物等;用于软骨组织工程的生长因子主要包括转化生长因子、骨形成蛋白、成纤维细胞生长因子、胰岛素样生长因子等.结论:迄今为止,无论是工程软骨的种子细胞、支架材料、培养环境等还没有任何一种材料被认为最理想,寻求一种具有良好性能的组织工程化关节软骨是未来研究的重点.但目前很多研究仍处于实验阶段,还有一些问题有待于解决,特别是组织工程细胞支架材料植入体内后,材料的降解与细胞功能发挥是否同步,会不会产生遗传物质改变、基因表达或基因突变等问题,将其应用于临床更需要相关学者专家不断的实践和探索.%OBJECTIVE: To summarize the application characteristics of tissue engineering technology and biomaterials in preventing sports articular cartilage injury.METHODS: A computer-based online search of Pubmed database (http://www.ncbi.nlm.nih.gov/PubMed) and VIP database (http://www.cqvip.com/) from

  18. Automatic classification of scar tissue in late gadolinium enhancement cardiac MRI for the assessment of left-atrial wall injury after radiofrequency ablation.

    Science.gov (United States)

    Perry, Daniel; Morris, Alan; Burgon, Nathan; McGann, Christopher; Macleod, Robert; Cates, Joshua

    2012-02-23

    Radiofrequency ablation is a promising procedure for treating atrial fibrillation (AF) that relies on accurate lesion delivery in the left atrial (LA) wall for success. Late Gadolinium Enhancement MRI (LGE MRI) at three months post-ablation has proven effective for noninvasive assessment of the location and extent of scar formation, which are important factors for predicting patient outcome and planning of redo ablation procedures. We have developed an algorithm for automatic classification in LGE MRI of scar tissue in the LA wall and have evaluated accuracy and consistency compared to manual scar classifications by expert observers. Our approach clusters voxels based on normalized intensity and was chosen through a systematic comparison of the performance of multivariate clustering on many combinations of image texture. Algorithm performance was determined by overlap with ground truth, using multiple overlap measures, and the accuracy of the estimation of the total amount of scar in the LA. Ground truth was determined using the STAPLE algorithm, which produces a probabilistic estimate of the true scar classification from multiple expert manual segmentations. Evaluation of the ground truth data set was based on both inter- and intra-observer agreement, with variation among expert classifiers indicating the difficulty of scar classification for a given a dataset. Our proposed automatic scar classification algorithm performs well for both scar localization and estimation of scar volume: for ground truth datasets considered easy, variability from the ground truth was low; for those considered difficult, variability from ground truth was on par with the variability across experts.

  19. 颜面部软组织创伤的急诊美容修复与护理%Plastic surgery and nursing for repairing of facial soft tissue injury

    Institute of Scientific and Technical Information of China (English)

    朱瑜; 李琳

    2015-01-01

    目的:探讨颜面部外伤的急诊美容修复与护理。方法:根据伤情,细心清创,对创面的处理方法做出正确的设计,采用肿胀麻醉,解剖对位,分层缝合,深层用0/5可吸收线减张缝合,皮肤用0/6单丝尼龙线缝合,缺损创面用带真皮下毛细血管网的皮肤或邻近皮瓣修复。结果:本组197处受伤部位,除了3例因皮肤挫伤缺损严重,待二期行肉芽创面植皮,其余病例均一期愈合。结论:颜面部软组织伤的急诊处理应遵循整形外科原则,术后精心护理,预防感染,减少颜面部的瘢痕、畸形、外伤性灰尘文身等医源性毁容。%Objective To observe the nursing and technique of plastic surgical repair in facial soft tissue trauma. Methods We repaired the wounds of emergency traumaticcases with aesthetic surgery methods and technique. Results The 197 wounds of emergency traumatic cases healed perfectly except the 3 cases with serious bruise were grafted skin on the granulation burn wounds. Conclusion Injury of facial superficial soft tissue might be repaired with cosmetic surgical operation on emergency and intensive perioperative nursing and we applied aesthetic surgery methods to repair the facial trauma can reduce postoperative scar abnormality and iatrogenic disfigure effectively.

  20. Paragliding injuries.

    OpenAIRE

    Krüger-Franke, M; Siebert, C H; Pförringer, W

    1991-01-01

    Regulations controlling the sport of paragliding were issued in April 1987 by the German Department of Transportation. The growing popularity of this sport has led to a steady increase in the number of associated injuries. This study presents the incidence, localization and degree of injuries associated with paragliding documented in Germany, Austria and Switzerland. The 283 injuries suffered by 218 paragliders were documented in the period 1987-1989: 181 occurred during landing, 28 during st...

  1. Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules.

    Science.gov (United States)

    Sadeghi, Hengameh; Lockmann, Anike; Hund, Anna-Carina; Samavedam, Unni K S R L; Pipi, Elena; Vafia, Katerina; Hauenschild, Eva; Kalies, Kathrin; Pas, Hendri H; Jonkman, Marcel F; Iwata, Hiroaki; Recke, Andreas; Schön, Michael P; Zillikens, Detlef; Schmidt, Enno; Ludwig, Ralf J

    2015-04-15

    Although reports documented aberrant cytokine expression in autoimmune bullous dermatoses (AIBDs), cytokine-targeting therapies have not been established in these disorders. We showed previously that IL-6 treatment protected against tissue destruction in experimental epidermolysis bullosa acquisita (EBA), an AIBD caused by autoantibodies to type VII collagen (COL7). The anti-inflammatory effects of IL-6 were mediated by induction of IL-1ra, and prophylactic IL-1ra administration prevented blistering. In this article, we demonstrate elevated serum concentrations of IL-1β in both mice with experimental EBA induced by injection of anti-COL7 IgG and in EBA patients. Increased IL-1α and IL-1β expression also was observed in the skin of anti-COL7 IgG-injected wild-type mice compared with the significantly less diseased IL-1R-deficient or wild-type mice treated with the IL-1R antagonist anakinra or anti-IL-1β. These findings suggested that IL-1 contributed to recruitment of inflammatory cells into the skin. Accordingly, the expression of ICAM-1 was decreased in IL-1R-deficient and anakinra-treated mice injected with anti-COL7. This effect appeared to be specifically attributable to IL-1 because anakinra blocked the upregulation of different endothelial adhesion molecules on IL-1-stimulated, but not on TNF-α-stimulated, cultured endothelial cells. Interestingly, injection of caspase-1/11-deficient mice with anti-COL7 IgG led to the same extent of skin lesions as in wild-type mice. Collectively, our data suggest that IL-1, independently of caspase-1, contributes to the pathogenesis of EBA. Because anti-IL-1β in a prophylactic setting and anakinra in a quasi-therapeutic setting (i.e., when skin lesions had already developed) improved experimental EBA, IL-1 appears to be a potential therapeutic target for EBA and related AIBDs.

  2. Cardiac functions assessment in children with celiac disease and its correlation with the degree of mucosal injury: Doppler tissue imaging study

    Directory of Open Access Journals (Sweden)

    Abeer Fathy

    2016-01-01

    Full Text Available Background/Aims: Celiac disease (CD-associated cardiologic disorders is a growing concern. However, data regarding cardiac affection in children with CD are few. This study aimed at assessing the subclinical impact of CD on the global myocardial performance in Saudi children with CD using Doppler tissue imaging (DTI. Patients and Methods: Conventional two-dimensional echocardiography was performed among 20 Saudi children with CDas well as 20 age and sex-matched healthy controls. DTI were used to determine right ventricular (RV and left ventricular (LV Tei indexes. These findings were correlated with the Modified Marsh Classification of the histologic findings in CD. Results: LV and RV Tei indexes were significantly higher in children with CD than the control group (mean ± standard deviation: 0.47 ± 0.05 vs. 0.31 ± 0.18; P< 0.0005 and 0.51 ± 0.04 vs. 0.32 ± 0.05; P< 0.0001, respectively. RV Tei index was found to be positively correlated with the Modified Marsh Classification of CD (r = 0.7753, P< 0.0001. LV Tei index tended to be more affected in patients with more severe histologic findings, however, such relation did not reach statistical significance (r = 0.2479, P = 0.292. Fractional shortening did not correlate with the Modified Marsh Classification of histologic findings in CD patients (r= −0.11, P = 0.641. Conclusions: Subclinical myocardial dysfunction of both ventricles occurs in children with CD. The DTI method appears to be more sensitive than conventional two-dimensional echocardiography in the early detection of myocardial dysfunction in children with CD.

  3. 创伤性颅脑损伤后脑组织中TLR4表达的实验研究%Expression of Toll like receptor 4 in injured brain tissue after traumatic brain injury

    Institute of Scientific and Technical Information of China (English)

    李光照; 赵飞; 杨非; 杭春华

    2011-01-01

    目的 研究创伤性脑损伤(TBI)后损伤灶周围脑组织Toll样受体4(TLR4)的表达,探讨TLR4/NF-κB信号通路在TBI中的作用机制.方法 SD大鼠36只按随机数字表法分为对照组(n=12)、TBI后1d组(n=6)、TBI后3d组(n=12)和TBI后7d组(n=6),后3组采用Feeney自由落体撞击法制作TBI模型,对照组仅行右侧顶部开窗而无TBI.应用RT-PCR、凝胶电泳迁移率实验(EMSA)、ELISA分别检测4组大鼠挫伤脑组织TLR4 mRNA、NF-κB活性、TNF-α和IL-6浓度的变化;免疫组化染色检测对照组和TBI后3d组大鼠挫伤脑组织TLR4的表达.结果 与对照组比较,TBI后1d、3d、7d组TLR4 mRNA表达、NF-κB活性、TNF-α和IL-6浓度均增加,差异有统计学意义(P<0.05);对照组脑组织TLR4表达较少,TBI后3d组创伤灶周围可见大量TLR4阳性细胞,主要表达在皮层胶质细胞、神经元中;NF-κB活性与TLR4 mRNA的表达呈正相关关系(r=0.786,P=-0.000).TNF-α、IL-6与TLR4的表达也呈正相关关系(r=0.517,P=0.010;r=0.503,P=0.012).结论 TBI可引起损伤区脑组织TLR4的表达和下游NF-κB、促炎症因子水平的增加,TLR4/NF-κB信号通路可能在脑组织的继发性损害中起重要作用.%Objective To investigate the expression of Toll like receptor 4(TLR4)in the injured brain tissue atter traumatic brain injury(TBI) and explore the potential role of TLR4/NF-κB in the secondary brain injury.Methods Thirty-six SD rats were randomly divided into control group(n=1 2),TBI inducement for 1 d group(n=6),TBI inducement for 3 d group(n=12)and TBI inducement for 7 d group(n=6).TBI models of the later 3 groups were induced by Feendy's free-falling,and rats of the control group are only performed exposure ofdura of the right parietal lobe.TLR4 mRNA expression in the injured brain tissue was studied by RT-PCR,NF-κB binding activity was detected by electrophoretic mobility shift assay (EMSA),and the TNF-α and IL-6 levels were detected by enzyme linked

  4. 组织工程化韧带在前交叉韧带运动损伤修复中的作用%Tissue engineered ligament in repair of sports-induced anterior cruciate ligament injury

    Institute of Scientific and Technical Information of China (English)

    许荣梅

    2011-01-01

    背景:交叉韧带是膝关节内的核心性稳定结构,具有制导膝关节生理活动并限制非生理性活动的功能,其中前交叉韧带的作用更为重要,其损伤的治疗已成为目前运动医学研究的热点.目的:探讨前交叉韧带生理功能、损伤机制,综述其修复过程中组织工程化材料的研究成果.方法:应用计算机检索1990-01/2011-02 PubMed数据库及维普数据库有关前交叉韧带组织工程研究进展、肌腱支架材料生物力学分析、生物材料在肌腱组织工程中应用及组织工程技术在修复肌腱缺损临床应用方面的相关文献,英文检索词"anterior cruciate ligament,biological materials,damage,treatment",中文检索词"前交叉韧带,生物材料,损伤,治疗",检索文献量总计102篇.结果与结论:前交叉韧带损伤后组织工程化康复措施得到了较快发展,从材料的选择来看,单一移植材料难免会存在诸多不足,不利于韧带的康复,复合材料可以结合不同材料的特性对韧带组织工程化材料进行配置,可以弥补单一材料的生物相容性、降解速度、生物力学性能、材料的韧性等不足,另外,对组织工程化材料进行功能结构加工也是尤为重要,这样可以更大程度的为细胞提供吸附、成长和分化的良好环境.同时,基因技术的进步以及各种新型材料的研制,必将在更大程度上满足前交叉韧带康复的需要.%BACKGROUND: Cruciate ligaments are the core of the knee joint, which can guide physical activity and limit non-physiological functions of the joint. The anterior cruciate ligament is especially important, and anterior cruciate ligament injury has become the hotspot in sports medicine.OBJECTIVE: To investigate the achievements on the physiological functions, injury mechanism and tissue-engineered materials used in the repair of anterior cruciate ligament.MEHTODS: A computer-based search was conducted in PubMed and VIP databases for

  5. Paragliding injuries.

    Science.gov (United States)

    Krüger-Franke, M; Siebert, C H; Pförringer, W

    1991-06-01

    Regulations controlling the sport of paragliding were issued in April 1987 by the German Department of Transportation. The growing popularity of this sport has led to a steady increase in the number of associated injuries. This study presents the incidence, localization and degree of injuries associated with paragliding documented in Germany, Austria and Switzerland. The 283 injuries suffered by 218 paragliders were documented in the period 1987-1989: 181 occurred during landing, 28 during starting procedures and nine during flight. The mean patient age was 29.6 years. There were 34.9% spinal injuries, 13.4% upper extremity injuries and 41.3% lower limb injuries. Over half of these injuries were treated surgically and in 54 instances permanent disability remained. In paragliding the lower extremities are at greatest risk of injury during landing. Proper equipment, especially sturdy footwear, exact training in landing techniques as well as improved instruction in procedures during aborted or crash landings is required to reduce the frequency of these injuries.

  6. Application of sustained compression cold therapy in early stage patients of soft tissue injury%持续加压冷疗在软组织损伤早期患者中的应用

    Institute of Scientific and Technical Information of China (English)

    梁妮; 林少虹; 杨克勤; 韦平欧; 陆彩玲; 何汉萍; 李春容; 黄式环

    2016-01-01

    Objective:To explore the safety of the application of sustained compression cold therapy in soft tissue injury. Methods:Selected 101 patients of closed injury of limbs patients applied with sustained compression cold therapy admitted by our hospital from January 2013 to May 2015 as research objects, monitored patients’1,2,4,24,48 h axillary temperature,body surface temperature of injured limbs and healthy limbs,pulse,blood pressure,blood oxygen saturation of endings before and after the cold therapy,recorded adverse events in cold therapy. Results:Body surface temperature of injured limbs after the cold therapy was lower than the temperature before the therapy,and there was no difference in all time period after the cold therapy(P > 0. 05);the pulse after the cold therapy was lower than the temperature before(P 0. 05);blood pressure after the cold thera-py was lower than the one before,and the blood pressure in all time period after the cold therapy were the same(P > 0. 05). There wasnˊt statistical differ-ence in axillary temperature,body surface temperature of healthy limbs,blood oxygen saturation of endings comparison(P > 0. 05);no adverse reaction oc-curred. Conclusion:Applying sustained compression cold therapy in early stage of soft tissue injury has safety.%目的:探讨持续加压冷疗在软组织损伤早期应用的安全性。方法:选择2013年1月~2015年5月期间我院收治的101例四肢闭合性损伤应用持续加压冷疗的患者为研究对象,监测冷疗前及冷疗后1,2,4,24,48 h 腋窝温度、患肢体表温度、健肢体表温度、脉搏、血压、末梢血氧饱和度,记录冷疗过程中的不良事件。结果:患肢体表温度冷疗后较冷疗前低,冷疗后各个时间段差异无统计学意义(P >0.05);脉搏在冷疗后比冷疗前低(P <0.05),冷疗后4,24,48 h 比冷疗后1 h 低(P <0.05),冷疗后24,48 h 变化差异无统计学意义(P >0.05);

  7. Iatrogenic Lens Injuries

    Directory of Open Access Journals (Sweden)

    Ümit Kamış

    2012-12-01

    Full Text Available During intraocular surgery, undesired damages of various etiology may occur in adjacent tissues. One of these tissues is the crystalline lens, which may be traumatized both in anterior segment and posterior segment surgeries, and when damaged, it usually causes marked decrease in visual acuity. The leading causes of iatrogenic lens injuries are intravitreal injection, laser iridotomy, phakic intraocular lens implantation, anterior chamber paracentesis, and vitreoretinal surgery. When crystalline lens damage occurs, its negative effect on visual function may be eliminated by performing cataract surgery intraoperatively or in elective conditions. (Turk J Ophthalmol 2012; 42: Supplement 27-30

  8. Effect of intestinal ischemia-reperfusion injury on the leptin levels in serum and adipose tissue%肠缺血再灌注损伤对血清及脂肪组织Leptin水平的影响

    Institute of Scientific and Technical Information of China (English)

    林季; 颜光涛; 王录焕; 郝秀华; 张凯; 薛辉

    2004-01-01

    BACKGROUND: Recent studies suggest that adipose tissue is not only the reservation of fat, but also a multi-potential endocrine organ secreting many functional cytokines. Leptin is a protein specifically secreted by adipose tissue, and primarily reduces food-intake and promotes energy expenditure.OBJECTIVE: To explore the effect of intestinal ischemia-reperfusion injury on leptin levels in serum and adipose tissue, and find out the role of leptin playing in acute inflammatory responses.DESIGN: A completely random self-and mutual-control experiment.SETTING and MATERIALS: The experiment was collected and completed in Research Laboratory of Biochemistry, General Hospital of Chinese PLA.Three male New Zealand white rabbits and fifty-four male Sprague-Dawley rats were used for experimental use.INTERVENTIONS: New Zealand white rabbits were immunized to obtain anti-leptin, leptin antigen was iodinated by chloramines-T method, thus we established a concise radioimmunoassay for murine leptin. An intestinal ischemia-reperfusion injury model of rats was established, and rats were divided randomly into six groups: sham-operation group(sham), 60-minute ischemia and 30-minute reperfusion group(I60-R30), I60-R90, I60-R150,I60-R240 and I60-R360; each group contained nine rats.MAIN OUTCOME MEASURES: The changes of leptin concentrations in serum and adipose tissue before and after ischemia-reperfusion injury were checked by the radioimmunoassay.RESULTS: Compared with self-control(before injury), serum leptin level of I60-R30 decreased significantly(t=2.3891, P<0.05), that of I60-R150 expressed a trend to increase and that of I60-R360 increased significantly ( t = - 2. 3437, P<0.05 ). Compared with sham after injury [ (9.88±1.87)μg/L], serum leptin level of I60-R240 expressed a trend to increase, that of I60-R360[ (19.43±2.84) μg/L] increased significantly( t = - 2. 8085, P <0.05) . Compared with sham after injury [ ( 11.12±1.27 ) ng], adipose leptin levels of I60-R30[ (4

  9. Effects of Acanthopanax senticosus on learning and memory in a mouse model of Alzheimer's disease and protection against free radical injury to brain tissue

    Institute of Scientific and Technical Information of China (English)

    Yanji Xu; Chunji Han; Songji Xu; Xing Yu; Guozhe Jiang; Chunhua Nan

    2008-01-01

    BACKGROUND:Acanthopanax senticosus,a plant of the Araliaceae family,is used in traditional Chinese medicine.It can be used to replenish Qi,strengthen the spleen,tonify the kidney,and relieve mental strain.OBJECTIVE:To observe effects ofAcanthopanax senticosus on learning and memory in a mouse model of Alzheimer's disease and abnormal biochemical changes in the brain tissue.DESIGN:A completely randomized grouping,controlled animal experiment.SETTING:Department of Preventive Medicine,School of Basic Medical Sciences,Yanbian University.MATERIALS:A total of 50 Kunming mice,aged 1-1.5 months,equal numbers of males and females,were provided by the Laboratory Animal Center,Yanbian University Medical College.The study was performed in accordance with ethical guidelines for the use and care of animals.Acanthopanax was provided by Yanbian Chengda Pharmaceutical Co.,Ltd.Acanthopanax senticosus(0.5 kg)was soaked in water for 1 hour and transferred to 1.5 kg distilled water for extraction.It was boiled for 1 hour and extracted after 1 hour of boiling.The procedure was repeated 3 times.The extract was condensed to 500 mL and then adjusted to 500 and 1 000 g/L with water.Piracetam tablets were produced by Shandong Luoxin Pharmaceutical Corporation, China.Malonaldehyde(MDA),superoxide dismutase(SOD),and acetylcholinesterase(ACHE)kits were purchased from Nanjing Jiancheng Bioengineering Co.,Ltd.,China. METHODS:This study was performed at the Department of Preventive Medicine,School of Basic Medical Sciences,Yanbian University from January to June 2007.All mice were randomly divided into 5 groups with 10 mice in each:control group,model group,low-,and high-dose Acanthopanax senticosus-treated groups, and piracetam-treated group.All groups were administered 0.1 mL/10 g.In the control and model groups, mice were intragastrically administered saline each morning for 5 days prior to experimentation.Five days later,they were intraperitoneally perfused with saline and aluminum trichloride

  10. 左炔诺孕酮宫内缓释系统治疗子宫腺肌病研究进展%Research of Levonorgestrel-releasing Intrauterine System Treatment for Adenomyosis

    Institute of Scientific and Technical Information of China (English)

    隋孟松

    2011-01-01

    Levonorgestrel-releasing intrauterine system is a new hormonal intrauterine contraceptive system. The system have the long-term effective in releasing levonorgestrel. In recent yeats,it found that in addition to long-term contraceptive effects, the system also can treat some gynecological diseases, especially in the treatment of adenomyosis, with efficacy, long term effective and low side effects, etc. The mechanism of levonorgestrel and the progress in the clinical application in recent years were reviewed in this paper.%左炔诺孕酮宫内缓释系统作为一种新型激素宫内避孕系统,长期有效释放左炔诺孕酮发挥作用.近年来发现其除了具有长期避孕作用外,对一些妇科疾病具有治疗作用,尤其在子宫腺肌病的治疗中,具有疗效明显、作用时间长及不良反应小等优点.现就近年来左炔诺孕酮的作用机制及在临床上的应用进展进行综述.

  11. Whiplash injuries.

    Science.gov (United States)

    Malanga, Gerard; Peter, Jason

    2005-10-01

    Whiplash injuries are very common and usually are associated with rear-end collisions. However, a whiplash injury can be caused by any event that results in hyperextension and flexion of the cervical spine. These injuries are of serious concern to all consumers due to escalating cost of diagnosis, treatment, insurance, and litigation. Most acute whiplash injury cases respond well to conservative treatments, which result in resolution of symptoms usually within weeks to a few months after the injury occurred. Chronic whiplash injuries often are harder to diagnose and treat and often result in poor outcomes. Current research shows that various structures in the cervical spine receive nociceptive innervation and potentially may be the cause of chronic pain symptoms. One potential pain generator showing promise is the facet or zygapophyseal joints. Various researchers have proven that these joints are injured during whiplash injuries and that diagnosis and temporary pain relief can be obtained with facet joint injections. The initial evaluation of any patient should follow an organized and stepwise approach, and more serious causes of neck pain must first be ruled out through the history, physical examination, and diagnostic testing. Treatment regimens should be evidence-based, focusing on treatments that have proven to be effective in treating acute and chronic whiplash injuries.

  12. Ocular Injury

    Science.gov (United States)

    ... eye and face protection is essential to prevent injuries. Sports such as hockey, baseball, racquet ball, squash, and shooting require protective goggles or full face mask wear at all times. Do fireworks still cause eye injuries? Each year hundreds of individuals (often children) sustain ...

  13. 子宫腺肌病患者泌尿系统症状发生情况及对生活质量的影响%Effect of the Incidence of Urinary Symptoms on Quality of Life of Patients with Adenomyosis

    Institute of Scientific and Technical Information of China (English)

    解红敏

    2014-01-01

    【目的】研究子宫腺肌病患者泌尿系统症状发生情况及对生活质量的影响。【方法】随机选取自2012年12月至2013年12月就诊于本院并行妇科超声检查的子宫腺肌病患者,共90例(即子宫腺肌病组)。对照组为超声检查示子宫肌层结构均正常的健康查体者,共90例。所有受试者均行盆腔检查,填写泌尿生殖尿失禁困扰量表(UDI-6)和尿失禁影响问卷(IIQ-7)两项调查问卷,并记录其泌尿系统症状发生情况。【结果】与对照组相比,子宫腺肌病组的压力性尿失禁,尿急,日间尿频,急迫性尿失禁和性交痛等泌尿系统症状的发生率显著较高,且两组相比较差异均有显著性(P<0.05)。子宫腺肌病组的 UDI总得分显著高于对照组(P<0.05),且子宫腺肌病组的与社会关系相关问题的 IIQ得分亦显著高于对照组(P<0.05)。【结论】子宫腺肌病患者尿路功能障碍发生率较高,且泌尿系统症状可能会影响子宫腺肌病患者的生活质量。%[Obj ective]To explore the incidence of urinary symptoms in patients with adenomyosis and the influence on the patients'quality of life(QOF).[Methods]Totally 90 patients with adenomyosis diagnosed by gynecological ultrasonography in our hospital from Dec.2012 to Dec.2011 were randomly selected as adeno-myosis group.Other 90 healthy subjects with showed by normal myometrial structure were selected as control group.All patients received pelvioscopy and filled two questionnaires such as urogenital distress inventory (UDI-6)and incontinence impact questionnaire(IIQ-7).The incidence of urinary symptoms was recorded.[Results]Compared with control group,the incidence of urinary symptoms such as stress urinary incontinence, urgency,daytime frequent micturition,urgent urinary incontinence and algopareunia in adenomyosis group was markedly increased,and there was significant difference between two groups

  14. Time profiles of the expression of metalloproteinases, tissue inhibitors of metalloproteases, cytokines and collagens in hamsters infected with Opisthorchis viverrini with special reference to peribiliary fibrosis and liver injury.

    Science.gov (United States)

    Prakobwong, Suksanti; Pinlaor, Somchai; Yongvanit, Puangrat; Sithithaworn, Paiboon; Pairojkul, Chawalit; Hiraku, Yusuke

    2009-06-01

    The liver fluke Opisthorchis viverrini is endemic in southeastern Asia, and causes cholangiocarcinoma and liver fibrosis. We investigated the time profile of the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) in relation to peribiliary fibrosis in O. viverrini-infected hamsters. Hepatic mRNA expression of MMPs, TIMPs, cytokines and collagens I and III was assessed by quantitative reverse transcription-PCR. Zymography and immunohistochemistry were also used to examine MMPs-2 and -9 expression. After infection, an increase of peribiliary fibrosis was time-dependent. Opisthorhis viverrini-induced gene expression in hamster liver, with increased mRNA expression levels of IL-1beta, TNF-alpha, TGF-beta, and collagens I and III, was observed at 21 days p.i. Expression of MMPs-2, -13 and -14 and TIMPs-1 and -3 genes, was significantly higher at 1 month, and maximal levels of most MMPs (MMPs-2, -9, -13 and -14) were observed at 2 months p.i. The cytoplasmic levels of MMP-2 and MMP-9 were similar to mRNA expression. Immunohistochemistry revealed that MMP-9 was expressed mainly in the cytoplasm of inflammatory cells at the invasive front of the fibrous area. In contrast, the highest levels of mRNA expression of TIMPs-2 and -3, and TGF-beta were observed 10 months p.i. Concentration of TIMP-2 protein in the plasma correlated with its transcriptional level (r=0.320, P=0.040). Peribiliary fibrosis correlated positively with liver hydroxyproline content (r=0.846, P<0.001), plasma hydroxyproline concentration (r=0.770, P<0.001), plasma TIMP-2 level (r=0.335, P=0.046), and mRNA expression levels of MMP-7 (r=0.511, P=0.006), TIMP-1 (r=0.320, P=0.040), TIMP-2 (r=0.428, P=0.026), and TIMP-3 (r=0.553, P=0.003). This study suggests that expression of MMPs is associated with an inflammatory reaction in the early phase and TIMPs expression at the late phase may contribute to both fibrosis and liver injury. MMPs and TIMPs may serve as diagnostic

  15. 中草药配合中医推拿对龙狮队员软组织损伤后的功能恢复%The effect of massage with Traditional Chinese Medicine on soft tissue rehabilitation after injury for the dragon-lion dance team members

    Institute of Scientific and Technical Information of China (English)

    林昭绒; 刘永峰; 欧光强; 章要在

    2015-01-01

    Based on the Dragon and Lion Dance team of Foshan University, through closed soft tissue injury investigation, we found the site of injury which had a close relationship with special technical features. In the Dragon and Lion Dance team functional recovery from soft tissue injury, this article mainly uses Traditional Chinese Medicine treatment such as massage therapy combined with TCM. Such therapy helps team members recover from injury as soon as possible, and maintain normal levels of physical health, as well as achieve the aim of motor skills recovery.%通过对佛山科学技术学院龙狮队员闭合性软组织损伤的调查,发现损伤的部位程度与专项技术特点有密切关系。在龙狮队员软组织损伤患者进行功能恢复中,主要采用中药疗法配合中医推拿等方法进行治疗。使龙狮队员肢体患处功能得到尽快的恢复,既可维持肢体健康的正常水平,又能达到恢复运动技能的目的。

  16. Spinal cord injury and MicroRNA:in vitro prefabrication of tissue-engineered spinal cord and repair of spinal cord injury%脊髓损伤与MicroRNA:体外预构组织工程化脊髓及损伤脊髓修复中的作用

    Institute of Scientific and Technical Information of China (English)

    李觅; 姚猛

    2015-01-01

    RNA expression induced by injuries as wel as the pathophysiological significance in spinal cord injury, and to explore the development potential of microRNA in tissue-engineered and clinical repair of spinal cord injury. METHODS:A computer-based search of PubMed and Chinese Journal Database was performed for related articles published from January 2000 to December 2014 using the keywords of “SCI, microRNA, transcriptional control, clinical research progress” in English and Chinese. Finaly, 38 articles were included for result analysis. RESULTS AND CONCLUSION: Mechanical injury initialy triggers a series of complex secondary damages, including nervous, vascular and immune systems, which can influence the severity of spinal cord injury to a great extent. Secondary damage to the spinal cord is mainly attributed to the activation and deactivation of some specific genes associated with celular and biochemical mechanisms, such as cysteine aspartate specific protease (caspase) gene family, apoptosis related protein Fas and its ligand Fasl system, P53 gene, apoptosis related gene Bcl-2 family. Recent studies have proved that the functional activation of microRNA expression is the key to spinal cord injury. With the development of biological information engineering, studies and controling technologies associated with microRNA expression have been gradualy dominated, some clinical application based on microRNA technology has entered the clinical trial stage. It is believed that with the continuous development of technology and decrease of cost, permanent dysfunction due to spinal cord injury can be regulated and repaired through the microRNA technology at gene level in the future.

  17. Application analysis of levonorgestrel-releasing intrauterine device in the treatment of young and middle-aged patients with adenomyosis%左炔诺孕酮宫内节育器在中青年子宫腺肌症治疗中的应用分析

    Institute of Scientific and Technical Information of China (English)

    徐红云

    2015-01-01

    目的 探讨临床上使用左炔诺孕酮宫内节育器治疗中青年子宫腺肌症的疗效. 方法 选取2013年1月至2014年9月鄢陵县人民医院收治的24例子宫腺症患者为研究对象,于宫内置左炔诺孕酮宫内节育器,随访1年,观察记录节育器放置前和放置后4、8、12 个月时患者的月经量、痛经程度、子宫大小、子宫内膜厚度、血清CA125及性激素变化情况. 结果 连续放置1年后患者月经量显著减少、痛经程度明显减轻、子宫大小和子宫内膜厚度显著降低、CA125水平显著降低,与放置前比较差异有统计学意义( P0.05). 结论 左炔诺孕酮宫内节育可显著改善中青年子宫腺肌症患者的月经量并缓解痛经,是一种较好的治疗中青年子宫腺肌症的保守治疗手段.%Objective To investigate the clinical effect of levonorgestrel-releasing intrauterine device on young and middle-aged patients with adenomyosis .Methods Twenty-four patients with adeno-myosis from January 2013 to September 2014 were selected and treated by levonorgestrel-releasing intrau-terine device .The menstrual capacity , dysmenorrhea of the degree , uterine size , endometrial thickness , serum CA125 and serum sex hormone levels were detected before and 4 , 8 , 12 months after treatment . Results After treatment for 12 months, the menstrual capacity , dysmenorrhea of the degree , uterine size, endometrial thickness, serum CA125 were changed significantly (P0.05).Conclusions The application of levonorgestrel-releasing intrauterine device is good to im-prove the clinical symptoms such as menstrual capacity and dysmenorrhea of the degree in adenomyosis , which can be a good conservative treatment method for the treatment of young and middle-aged patients with adenomyosis .

  18. Biomaterials in the repair of sports injuries

    Science.gov (United States)

    Ducheyne, Paul; Mauck, Robert L.; Smith, Douglas H.

    2012-08-01

    The optimal stimulation of tissue regeneration in bone, cartilage and spinal cord injuries involves a judicious selection of biomaterials with tailored chemical compositions, micro- and nanostructures, porosities and kinetic release properties for the delivery of relevant biologically active molecules.

  19. 应用引导组织再生术修复外伤后牙槽骨缺损的临床研究%Treatment of periodontal defects following tooth injury by guided tissue regeneration technique

    Institute of Scientific and Technical Information of China (English)

    许杰; 邓再喜; 马志伟; 董广英; 刘青; 储庆; 王新文; 吴织芬; 吴秀珍

    2011-01-01

    Objective: To study the feasibility of guided tissue regeneralion( CTR) in the treatment of periodontal bone defects following tooth injury. Methods; 8 patients (4 male and 4 female) , altogether 11 teeth with periodontal defects,received the surgery for repair of the defects by Bio-Oss and Heal-all membrane transplantation. Probing pocket depth ( PPD) , probing attachment level (PAL) and gingival recession (GR) were measured before treatment, 12 weeks and 24 weeks after treatment. Radiographic measurements included the area of the dark space between two adjacent teeth, polygon area ( PG) , and the length from the root apex lo the deepest point of angular bone defect along the tooth root surface ( L) were analysed by Image Pro- Plus software. The complications were observed after the surgery. Results; Treatment was completed in all the patients, PPD and PAL values reduced 12 and 24 weeks after treatment(P 0.8 (a >0. 4). Conclusion: The application of GTR technique by using Bio-Oss and Heal-all membrane may be promising in ihe treatment of periodontal defects after traumatic dental injury.%目的:研究引导组织再生术(guided tissue regeneration,GTR)修复牙外伤后牙槽骨缺损的可行性.方法:纳入8例前牙外伤患者(4男,4女),共11处前牙邻间隙牙槽骨角形吸收,牙周基础治疗4~6周后接受GTR术,植入Bio-oss骨移植材料和Heal-all屏障膜.记录术前、术后牙周临床指标,并采用Image Pro-Plus图像分析软件对平行投照X线牙片中骨缺损区内邻间隙阴影面积(polygon area,PG)和牙根尖至角形骨吸收牙槽嵴最低点之间的牙根外表面曲线长度(length of tooth root surface L)进行定量分析.结果:所有患者的治疗都取得了满意的效果.术后12、24周的牙周探诊深度(PPD)和探诊附着丧失(PAL)都较术前明显减少(P<0.05),同时也出现了一定程度的牙龈退缩(P<0 05).X线片影像分析显示,在术后12、24周时,反映牙槽骨缺损范围的PG值

  20. Cellular proliferation and regeneration following tissue damage. Progress report. [Eyes

    Energy Technology Data Exchange (ETDEWEB)

    Harding, C.V.

    1976-10-01

    Results are reported from a study of wound healing in tissues of the eye, particularly lens, cornea, and surrounding tissues. The reactions of these tissues to mechanical injuries, as well as injuries induced by chemotoxic agents were studied. It is postulated that a better understanding of the basic reactions of the eye to injurious agents may be of importance in the evaluation of potential environmental hazards.

  1. A phase I study evaluating the pharmacokinetics, safety and tolerability of an antibody-based tissue factor antagonist in subjects with acute lung injury or acute respiratory distress syndrome

    Directory of Open Access Journals (Sweden)

    Morris Peter E

    2012-02-01

    Full Text Available Abstract Background The tissue factor (TF-dependent extrinsic pathway has been suggested to be a central mechanism by which the coagulation cascade is locally activated in the lungs of patients with acute lung injury and acute respiratory distress syndrome (ALI/ARDS and thus represents an attractive target for therapeutic intervention. This study was designed to determine the pharmacokinetic and safety profiles of ALT-836, an anti-TF antibody, in patients with ALI/ARDS. Methods This was a prospective, randomized, placebo-controlled, dose-escalation Phase I clinical trial in adult patients who had suspected or proven infection, were receiving mechanical ventilation and had ALI/ARDS (PaO2/FiO2 ≤ 300 mm. Eighteen patients (6 per cohort were randomized in a 5:1 ratio to receive ALT-836 or placebo, and were treated within 48 hours after meeting screening criteria. Cohorts of patients were administered a single intravenously dose of 0.06, 0.08 or 0.1 mg/kg ALT-836 or placebo. Blood samples were taken for pharmacokinetic and immunogenicity measurements. Safety was assessed by adverse events, vital signs, ECGs, laboratory, coagulation and pulmonary function parameters. Results Pharmacokinetic analysis showed a dose dependent exposure to ALT-836 across the infusion range of 0.06 to 0.1 mg/kg. No anti-ALT-836 antibody response was observed in the study population during the trial. No major bleeding episodes were reported in the ALT-836 treated patients. The most frequent adverse events were anemia, observed in both placebo and ALT-836 treated patients, and ALT-836 dose dependent, self-resolved hematuria, which suggested 0.08 mg/kg as an acceptable dose level of ALT-836 in this patient population. Conclusions Overall, this study showed that ALT-836 could be safely administered to patients with sepsis-induced ALI/ARDS. Trial registration ClinicalTrials.gov: NCT01438853

  2. Informing tendon tissue engineering with embryonic development

    OpenAIRE

    Glass, Zachary A.; Schiele, Nathan R.; Kuo, Catherine K

    2014-01-01

    Tendon is a strong connective tissue that transduces muscle-generated forces into skeletal motion. In fulfilling this role, tendons are subjected to repeated mechanical loading and high stress, which may result in injury. Tissue engineering with stem cells offers the potential to replace injured/damaged tissue with healthy, new living tissue. Critical to tendon tissue engineering is the induction and guidance of stem cells towards the tendon phenotype. Typical strategies hav...

  3. 尼莫同对大鼠创伤性脑损伤后脑组织NF-κB表达和活化的影响%Effect of nimotop on expression and activation of NF-κB in the rat brain tissues after traumatic brain injury

    Institute of Scientific and Technical Information of China (English)

    陈兵; 吴华伟; 尹延庆; 彭浩; 龙霄翱; 梁远生; 徐军发; 陈东

    2009-01-01

    Objective To explore the effect of nimotop on expression and activation of NF-κB after traumatic brain injury. Methods Local traumatic brain injury was made in rats by strike with freefalling hammer. Then, the rats were injected with dexamethasone and nimotop respectively to detect expressions of NF-κB P65 and IκBα in rat brain tissues by means of immunohistochemistry. Results The expression of NF-κB P65 in brain tissue was obviously increased after traumatic brain injury and reached peak at 24 hours, which lasted for 96 hours. The expression of IκB was obviously decreased after traumatic brain injury and lowered to the least at 24 hours. Both dexamethasone and nimotop could decrease the expression of NF-κB P65 and increase the expression of IκBα in rat brain tissues after traumatic brain injury (P<0.01), when nimotop was better than dexamethasone (P<0.05). Conclusions Nimotop can inhibit the expression and activation of NF-κB and hence regulate inflammatory reaction after traumatic brain injury.%目的 观察尼莫同对大鼠创伤性脑损伤(traumatic brain injury,TBI)后NF-κB表达和活化的影响. 方法制作SD大鼠落体撞击脑损伤模型,分别给予地塞米松和尼莫同干预,检测脑组织NF-κB P65和IκBα的表达. 结果 TBI后脑组织NF-κB P65的表达明显增加,伤后24 h最低,持续到96 h仍有明显表达;IκBα表达明显减弱,伤后24 h达到低峰.尼莫同与地塞米松可以显著降低TBI后NF-κB P65的表达和增加IκBα的表达(P<0.01),尼莫同作用优于地塞米松(P<0.05). 结论尼莫同可以抑制TBI后NF-κB表达和活化,从而调节脑损伤后的炎症反应.

  4. MESS评分在肢体严重软组织损伤治疗中的应用评估%A Retrospective Assessment of the Application Value of MESS in the Treatment of Severe Limb Soft Tissue Injuries

    Institute of Scientific and Technical Information of China (English)

    何志; 李靖; 蒋立; 冯剑; 吕小星; 孙超峰; 李跃军; 李学拥; 黄辞

    2015-01-01

    Objective:To investigate the application value of MESS in the treatment of severe limb soft tissue injuries.Methods:The clinical data of 50 cases of patients with severe extremity soft tissue injuries who were treated in our division from August 2010 to May 2014 were retrospectively analyzed,including 34 males and 16 females,aged 23-53,with the average of 38.Each case was scored ac-cording to the protocol of MESS,and received debridement,limb salvage or amputation operation and repair operation according to his or her specific condition.The patients were followed up for 3 months to 3 years.All the cases were grouped according to their outcomes to limb-salvage group,first stage amputation group and secondary amputation group.The MESS score,hospital stay,operation times,incidence rate of complications and satisfaction rate were compared among the three groups.Results:32 patients in limb-salvage group had MESS score of 6-11 (8.63 ± 1.26).18 cases received amputation surgery had MESS score of 1 1-14,including 10 cases of first stage amputation group (MESS 12.60± 0.97) and 8 cases of secondary amputation group (12.88± 0.83).MESS score of limb-salvage group was significantly lower than that of the first stage amputation group (P<0.05) and the secondary amputation group (P<0.05).In addition,the satisfaction rate of limb-salvage group was higher than that of the first stage amputation group (P<0.05) and the secondary amputation group (P<0.05).The hospital stay,operation times and incidence rate of complications of first stage amputation group were all lower than those of the secondary amputation group.The patients' satisfaction rate of first stage amputation group was higher than that of the secondary amputation group (P<0.05).Conclusion:Severe limb soft tissue injuries patients with the MESS score<1 1 points got satisfactory results with the limb salvage operations.While for patients with the MESS score> 11 points,first stage amputation could have better

  5. Acute finger injuries: part II. Fractures, dislocations, and thumb injuries.

    Science.gov (United States)

    Leggit, Jeffrey C; Meko, Christian J

    2006-03-01

    Family physicians can treat most finger fractures and dislocations, but when necessary, prompt referral to an orthopedic or hand surgeon is important to maximize future function. Examination includes radiography (oblique, anteroposterior, and true lateral views) and physical examination to detect fractures. Dislocation reduction is accomplished with careful traction. If successful, further treatment focuses on the concomitant soft tissue injury. Referral is needed for irreducible dislocations. Distal phalanx fractures are treated conservatively, and middle phalanx fractures can be treated if reduction is stable. Physicians usually can reduce metacarpal bone fractures, even if there is a large degree of angulation. An orthopedic or hand surgeon should treat finger injuries that are unstable or that have rotation. Collateral ligament injuries of the thumb should be examine with radiography before physical examination. Stable joint injuries can be treated with splinting or casting, although an orthopedic or hand surgeon should treat unstable joints.

  6. Surgical treatment of anterior cruciate ligament injury in adults.

    Science.gov (United States)

    Alazzawi, Sulaiman; Sukeik, Mohamed; Ibrahim, Mazin; Haddad, Fares S

    2016-04-01

    Anterior cruciate ligament injury is among the most common soft tissue injuries of the knee joint and reconstruction of the anterior cruciate ligament is the gold standard treatment for young active symptomatic patients. This review summarizes the surgical treatment of anterior cruciate ligament injury.

  7. Transcapillary transport after thermal injury.

    Science.gov (United States)

    Arturson, G; Jonsson, C E

    1979-01-01

    The pathophysiology of the burn wound is characterized by an inflammatory reaction leading to rapid edema formation due to (1) dilatation of resistance vessels with increased effective transcapillary filtration pressure, (2) increased extravascular osmotic activity created in damaged tissue, and (3) increased microvascular permeability to macromolecules. In extensive burns increased microvascular permeability was found also in tissues remote from the thermal injury. These reactions are due to direct heat effect on the microvasculature and to chemical mediators of inflammation. Important is the increased biosynthesis of prostaglandins at the site of tissue injury which may partly explain vasodilatation, increased microvascular permeability and accumulation of polymorphonuclear leucocytes observed following thermal injury. The morphological interpretations of the changes in the functional ultrastructure of the blood-lymph barrier following thermal injury seem to be a remarkable and persistant increase in the numbers of vacuoles and many open endothelial intercellular junctions. Further less explored changes of the interstitial tissue after severe burn trauma seem to be of great importance.

  8. 音乐治疗在高强度聚焦超声消融子宫腺肌症中的应用%Application of music therapy in ablation of uterine adenomyosis with high intensity focused ultrasound

    Institute of Scientific and Technical Information of China (English)

    吴莺; 高悦; 王燕; 金志春; 陈锦云

    2013-01-01

    Objective:To investigate the effects of music therapy on uterine adenomyosis patients accepting high intensity focused ultrasound (HIFU) ablation. Methods:A total of 90 adenomyosis patients accepting HIFU ablation were randomly divided into the music therapy group and the control group. Music therapy group continued to listen to music from 1 hour before the ablation to the the end of the ablation. The control group was according to the conventional methods for HIFU ablation. Two groups were compared by patients’ preoperative anxiety levels, intra-operative blood pressure and heart rate, post-operative pain and analgesic doses. Results:The preoperative anxiety degree, the intra-operative blood pressure and heart rate, the post-operative pain scores and analgesic doses in the music therapy group were significantly better than those in the control group. Conclusion:Music therapy in HIFU ablation can improve stress state of patients. It can stabilize intra-operative physiological indexes of patients, reduce their anxiety degree, reduce post-operative pain and analgesic drug doses as well, and it’s good for the smooth progress of HIFU ablation.%目的:研究音乐治疗在高强度聚焦超声消融子宫腺肌症中的应用效果。方法:将90例高强度聚焦超声(HIFU)消融治疗的子宫腺肌症患者随机分成实验治疗组和对照组进行研究分析。实验治疗组在常规消融治疗前1h至结束时持续听音乐;对照组则按常规方式进行超声消融治疗。观察二组患者治疗前的焦虑程度,治疗中的血压、心率,治疗后的疼痛及镇痛药物使用剂量情况。结果:实验治疗组治疗前的焦虑程度、治疗中的血压、心率、治疗后的疼痛评分、镇痛药物使用剂量均明显优于对照组。结论:应用音乐治疗可明显改善子宫腺肌症患者HIFU治疗过程中的应激状态,稳定治疗中生理指标,减轻焦虑状态及治疗后疼痛和镇痛药物使用剂

  9. 负压封闭引流术治疗四肢软组织严重挤压伤的效果%Effect of vacuum sealing drainage on treating severe crush injury of limbs soft tissue

    Institute of Scientific and Technical Information of China (English)

    杨威; 刘杨; 赵鸣雁

    2015-01-01

    目的:观察负压封闭引流术(VSD)对四肢软组织严重挤压伤的治疗效果。方法选择哈尔滨医科大学附属第一医院2012年10月~2014年10月收治的62例GustiloⅢ型软组织挤压伤患者,根据清创术后选择VSD引流或常规换药治疗分为观察组(35例)和对照组(27例),观察组清创术后安装VSD装置,对照组根据伤口情况每日换药1~2次。术后对患者行积极的全身治疗,并于术后48 h检测患者血浆白细胞(WBC)计数,血清钾离子(K+)、肌酐(Scr)、尿素氮(BUN)水平以及尿蛋白情况。观察患者出现急性肾损伤(AKI)、全身炎症反应综合征(SIRS)、脓毒症、多器官功能障碍综合征(MODS)并发症的发生率,并统计患者病死率。在患者出院时统计患者伤口闭合时间、血液净化次数、住院时间及截肢率等预后指标。结果观察组WBC[(13.25±3.16)×109/L]、K+[(5.04±1.07)mmol/L]、Scr [(163.43±53.27)μmol/L]、BUN [(6.92±2.61)mmol/L]、尿蛋白评分[(2.09±0.83)分]均优于对照组[WBC:(18.63±4.58)×109/L,K+:(5.93±1.42)mmol/L,Scr:(334.56±109.82)μmol/L,BUN:(10.35±3.48)mmol/L,尿蛋白评分:(2.67±1.12)分],差异有统计学意义(P0.05)。结论 VSD技术能够有效降低软组织损伤后局部有害物质的吸收,减轻全身中毒症状,提高治疗效果。%Objective To observe the therapeutic effect of vacuum sealing drainage (VSD) on treating severe crush in-jury of limbs soft tissue. Methods From October 2012 to October 2014, in the First Affiliated Hospital of Harbin Med-ical University, 62 GustiloⅢ crush injured patients were chosen and divided into observed group (n =35)and control group (n = 27), according to using VSD or dressing change after debridement. After debridement, the observed group was installed VSD, the control group was used dressing change 1-2 times/d. With the positive therapy, all the patients were detected including white blood cell count (WBC

  10. Effects of mechanical ventilation on cell apoptosis in lung tissue of rat with acute lung injury%机械通气对急性肺损伤大鼠细胞凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    陈宁; 赵鹤龄; 程彤; 申丽旻; 张耀宗

    2011-01-01

    Objective To investigate the effects of different tidal volumes and positive end expiratory pressures on cell apoptosis in lung tissue of rats with acute lung injury.Methods Forty healthy male Sprague-Dawley rats were randomly(random number)divided into five groups,namely low tidal volume group(LV,VT 8 mL/kg),high tidal volume group(HV,VT 30 mL/kg),low tidal volume group with PEEP 2cmH2O(LV2P,VT 8 mL/kg,PEEP 2 cmH2O),low tidal volume group and PEEP 5cmH2O (LV5P,VT 8 mL/kg,PEEP 5 cmH2O)and low tidal volume group and PEEP 8 cmH2O(LV8P,VT 8 mL/kg,PEEP 8 cmH2O).After intravenous administration of oleic acid(OA,0.1 mL/kg),the rat model of acute lung injury was made.Mechanical ventilation was employed in rats of the experiment groups.Rats were sacrificed and their whole lungs were taken after mechanical ventilation for 2 hours.The transferase d-UTP end labeling assay(TUNEL)was used to define the extent and distribution of apoptotic cells in bronchus and lung tissues.The level of caspase-3 protein was determined by immunohistochemistry.Results The apoptotic cells on both alveolar septum and bronchial epithelium obviously increased with high level of caaspase-3 protein in HV group.The number of apoptotic cells obviously decreased with decrease in caspase-3 protein after PEEP ventilation.These changes were more significant in LV5P than those in other groups(P < 0.01).Conclusions The mechanical ventilation with low tidal volume and PEEP produces protective effects on lung from injury.The cell apoptosis plays an important role in the course of VILI.%目的 研究不同潮气量及不同呼气末正压(PEEP)水平对急性肺损伤(AU)大鼠支气管和肺组织细胞凋亡的影响,并初步探讨细胞凋亡在呼吸机相关性肺损伤(VILI)中的作用机制.方法 选用40只SD大鼠,制作ALI模型,随机(随机数字法)分为:(1)小潮气量组(LV组),潮气量8 mL/kg,不加PEEP;(2)大潮气量组,潮气量30 mL./kg,不加PEEP;(3)小潮气量+ 2PEEP组(LV2P

  11. Ectopic Adipose Tissue Storage in the Left and the Right Renal Sinus is Asymmetric and Associated With Serum Kidney Injury Molecule-1 and Fibroblast Growth Factor-21 Levels Increase

    Directory of Open Access Journals (Sweden)

    Gita Krievina, MSc

    2016-11-01

    Conclusions: Regardless of gender adipose tissue in RS accumulates asymmetrically–the left RS accumulates a significantly higher amount of adipose tissue. Thus, primarily RS adipose tissue effects should be assessed on the left kidney. Accumulation of adipose tissue in the RS is related with the visceral adipose amount, KIM-1 and FGF-21 concentration increase in the blood serum.

  12. Injury potential in modern ice hockey.

    Science.gov (United States)

    Sim, F H; Chao, E Y

    1978-01-01

    The majority of the damaging forces to the soft tissue, bone, and articular joint structures of modern hockey players during the energetic activities involved in the game are attributable to impact action during high-speed motion. In addition, non-contact musculoligamentous injuries are common because of the complex forces that are involved. The injury potential of this sport is assessed indirectly from the force and motion involved. The experimental method of measuring the kinematic motion and the impact forces inherent to the sport are presented. Although hockey is a fast and furious game with high injury potential, fortunately the number of serious injuries is not as great as one might expect.

  13. Macrophages in Tissue Repair, Regeneration, and Fibrosis.

    Science.gov (United States)

    Wynn, Thomas A; Vannella, Kevin M

    2016-03-15

    Inflammatory monocytes and tissue-resident macrophages are key regulators of tissue repair, regeneration, and fibrosis. After tissue injury, monocytes and macrophages undergo marked phenotypic and functional changes to play critical roles during the initiation, maintenance, and resolution phases of tissue repair. Disturbances in macrophage function can lead to aberrant repair, such that uncontrolled production of inflammatory mediators and growth factors, deficient generation of anti-inflammatory macrophages, or failed communication between macrophages and epithelial cells, endothelial cells, fibroblasts, and stem or tissue progenitor cells all contribute to a state of persistent injury, and this could lead to the development of pathological fibrosis. In this review, we discuss the mechanisms that instruct macrophages to adopt pro-inflammatory, pro-wound-healing, pro-fibrotic, anti-inflammatory, anti-fibrotic, pro-resolving, and tissue-regenerating phenotypes after injury, and we highlight how some of these mechanisms and macrophage activation states could be exploited therapeutically.

  14. The effect of prolonged gonadotropin-releasing hormone agonist-induced down-regulation on pregnancy outcomes in frozen-thawed embryo transfer cycles for the patients with adenomyosis%促性腺激素释放激素激动剂超长降调节改善子宫腺肌病冻融胚胎移植妊娠结局的作用

    Institute of Scientific and Technical Information of China (English)

    冯云

    2011-01-01

    目的 评价促性腺激素释放激素激动剂(GnRH-a)超长垂体降调节对改善子宫腺肌病(AM)冻融胚胎移植(FET)周期妊娠结局的价值. 方法 将AM患者分为GnRH-a+人工周期组和单纯人工周期组,非AM患者行人工周期作为对照组.比较三组患者各项指标包括胚胎种植率、临床妊娠率、流产率、异位妊娠率差异. 结果 GnRH-a+人工周期子宫内膜准备方案明显提高AM患者FET周期胚胎种植率和临床妊娠率,而未增加流产率和异位妊娠率. 结论 GnRH-a超长降调节明显改善AM患者FET妊娠结局.%Objective:To evaluate the effect of prolonged gonadotropin-releasing hormone agonist (GnRH-a)-induced down-regulation on the outcomes of frozen-thawed embryo transfer (FET) cycles in the patients with adenomyosis.Methods:Patients with adenomyosis were divided into GnRH-a plus artificial cycle group (116 cycles) and simple artificial cycle group (106 cycles). Patients without adenomyosis who underwent artificial cycle before FET were grouped as controls (433 cycles). The pregnancy outcomes, including implantation rate, pregnancy rate, abortion rate and ectopic pregnancy rate of three groups were measured and compared.Results: GnRH-a down-regulation plus artificial cycle for endometrial preparation before FET significantly improved the implantation rate and pregnancy rate in adenomyosis patients, with no increase in the abortion rate or ectopic pregnancy rate.Conclusion: Prolonged GnRH-a therapy for down-regulation significantly improve the outcomes of FET cycles in adenomyosis patients.

  15. Aplicação de dispositivo intra-uterino liberador de levonorgestrel, previamente a ciclos de fertilização in vitro, nas portadoras de adenomiose Application of a levonorgestrel-releasing intrauterine device prior to in vitro fertilization cycles in women with adenomyosis

    Directory of Open Access Journals (Sweden)

    Nilson Donadio

    2006-08-01

    Full Text Available OBJETIVO: verificar os efeitos do dispositivo intra-uterino (DIU de levonorgestrel em portadoras de adenomiose, com falhas de implantação em ciclos de fertilização in vitro (FIV. MÉTODOS: foram selecionadas 80 mulheres inférteis, com até 38 anos, com diagnóstico de adenomiose pela ultra-sonografia e ressonância magnética pélvica. Todas apresentavam tentativas anteriores de FIV sem sucesso. No grupo Diu, de 40 mulheres, foi colocado DIU liberador de 20 µg de levonorgestrel/dia por seis meses, previamente a um novo ciclo de FIV. No grupo Fiv, de 40 mulheres, estas foram submetidas diretamente a novo ciclo, sem o prévio tratamento. No Grupo Diu, avaliaram-se o volume uterino, a espessura e os focos de hipersinal da zona juncional, pré e pós-tratamento, assim como as taxas de gravidez em novo ciclo de FIV comparadas com as obtidas no grupo Fiv. As análises estatísticas foram realizadas adotando-se o nível de significância de 5% (pPURPOSE: to verify the effects of intrauterine levonorgestrel device (IUD in women with adenomyosis, with implantation failure in previous in vitro fertilization (IVF cycles. METHODS: eighty infertile women with ages up to 38 years, who had adenomyosis diagnosed by ultrasonography and MRI were selected. All the women presented antecedents of one or more tormer IVF attempts without success due to implantation failure. The women were subdivided into IUD Group, composed of 40 women with an IUD that released 20 µg of levonorgestrel/day during six months, preceding a new IVF cycle, and IVF Group, also composed of 40 women, who were directly submitted to a new IVF cycle without previous adenomyosis treatment. In the IUD Group the uterine volume, thickness and hypersignal foci of the junctional zone were assessed before and after treatment, as well as the pregnancy rates in the new IVF cycle, compared to the data obtained with the IVF Group. Statistical analyses were performed adopting the significance level of

  16. Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

    OpenAIRE

    2011-01-01

    Traumatic brain injury (TBI) research has attained renewed momentum due to the increasing awareness of head injuries, which result in morbidity and mortality. Based on the nature of primary injury following TBI, complex and heterogeneous secondary consequences result, which are followed by regenerative processes 1,2. Primary injury can be induced by a direct contusion to the brain from skull fracture or from shearing and stretching of tissue causing displacement of brain due to movement 3,4. ...

  17. Chinese Massage Therapy for Ankle Injury

    Institute of Scientific and Technical Information of China (English)

    CHEN Ming-xia; LI Nian-qun; HUANG Guo-qi

    2003-01-01

    Purpose: To explore the therapeutic methods and effects in the treatment of acute and chronic injuries of the ankle joint by Chinese massage therapy in combination with external application of Chinese herbal drugs and functional exercises. Methods: Totally, 36 cases of the patients with acute soft tissue injury, chronic soft tissue injury and post-fracture sequelae of the ankle joint were treated by Chinese massage therapy, external application or external wash of Chinese herbal drugs, and exercises of dorsal flexion and extension of the ankle joint, to observe the restoration of the ankle functions.Results: In 36 cases of the patients, the results showed remarkable effect in 18 cases, effect in 16 cases, failure in 2 cases and the effective rate in 94.4%. Conclusion: The combined use of Chinese massage therapy, external application of Chinese herbal drugs and functional exercises can produce precise effect in the treatment of soft tissue injury of the ankle joint.

  18. Influence of indomethacin on free radical reactions in brain tissues ofrabbits with brain injury%消炎痛对兔脑外伤后脑组织氧自由基反应的影响

    Institute of Scientific and Technical Information of China (English)

    谭占国; 柴宗举; 冯祖荫

    2001-01-01

    目的:探讨消炎痛对急性实验性脑外伤后兔脑组织氧自由基反应的影响。方法:30只家兔随机分为A组(正常对照组)、B组(生理盐水治疗组)及C组(消炎痛治疗组),每组10只动物。其中A组不致脑外伤,作为正常对照。B、C2组采用自由落体打击法建立兔闭合性脑外伤模型。C组于外伤后.10min开始,经耳缘静脉缓慢注入消炎痛针剂。B组于外伤后相同时间同样方法注入等量的生理盐水,作为生理盐水治疗对照。B、C2组于外伤后4h将动物处死,完整取出大脑半球以干湿重比较法测定大脑含水量;取挫伤灶周边脑组织制成匀浆,生化测定脑组织中过氧化物歧化酶(SOD)活性和脂质过氧化物(LPO)含量。结果:B组动物致脑外伤后4h,脑组织含水量及LPO含量分别为(79.918±1.449)%和(143.5±24.9)nmol/g,较对照组显著为高(P0.05);SOD活性为(2.25±0.21)Nu/mg,较B组明显升高(P 0.05). The mean value of SOD activity was (2.25 + 0.21 ) Nu/mg, higher than that in group B ( P < 0.05). Conclusion:IM treatment at the early stage of brain injury could attenuate oxygen free radical reaction in injured brain tissues, protect bloodbrain barrier, prevent traumatic cerebral edema being formed and developing fmther.

  19. 软组织损伤评估处理对跟骨骨折术后感染的意义%Classified Treatments of Soft-tissue Injury in Preventing Postoperative Infection in Patients with Calca-neal Fracture

    Institute of Scientific and Technical Information of China (English)

    徐生根; 肖坚; 吴维剑

    2016-01-01

    Objective To study the clinical significance of soft tissue assessment and classification treat⁃ment for the prevention of postoperative infection in the patients with calcaneal fractures. Methods We re⁃cruited 91 patients who underwent open reduction and reconstruction plate internal fixation operation for closed calcaneal fractures (103 feet). All patients were randomly divided into a control group and an evaluation group. In the control group, there were 36 men (40 feet) and 7 women (8 feet). They aged from 18 to 72 years (average 38.5 years). By Sanders classification, 11 feet were of type Ⅱ, 21 feet of type Ⅲ and 8 feet of type IV. All con⁃trol patients with preoperative soft tissue injury underwent the conventional preparation processing, and preopera⁃tive assessment of soft tissue and classification treatment was not carried out. In the evaluation group, there were 39 men (44 feet) and 9 women (11 feet). They aged from 19 to 71 years(average 39.6 years). By Sanders classifi⁃cation,12 feet were of type Ⅱ, 23 feet of type Ⅲ, and 9 feet of type IV. They underwent soft tissue assessment using criteria for preoperative assessment and also underwent classification treatment. Results The control group of 43 patients were followed up for 13~17 months (average 14.5 months). There were 5 cases of postopera⁃tive wound infection, and the infection rate was 16.3%. In the evaluation group, 48 cases were followed up for 14-22 months (average 16.1 months), and only 2 cases had wound infection after surgery, and the infection rate was 4.17%. The infection rates in the two groups were different significantly (P<0.05). Conclusion It is im⁃portant to conduct preoperative skin and soft tissue assessments and classification treatment, as an intervention strategy for calcaneal fractures. It can significantly reduce the rate of wound infection after fracture of calcaneus.%目的:探讨皮肤软组织损伤评估及分级处理对预防跟骨骨折术后切

  20. Facial Sports Injuries

    Science.gov (United States)

    ... Find an ENT Doctor Near You Facial Sports Injuries Facial Sports Injuries Patient Health Information News media interested in ... should receive immediate medical attention. Prevention Of Facial Sports Injuries The best way to treat facial sports injuries ...

  1. [The hardness of the traumatic object and the extent of injury (as exemplified by head injuries)].

    Science.gov (United States)

    Shadymov, A B; Kazymov, M A

    2014-01-01

    The objective of the present work was to study the influence of the surface hardness of a traumatic agent on the extent and character of the injury to the soft and bone tissues of the cranial vault associated with various forms of injurious exposure. The authors evaluated forensic medical significance of the hardness as one of the most important properties of the major injurious agents involved in the formation of skull fractures and soft tissue ruptures under effect of an impact action or compression. The objects differing in the hardness of the contact element (striking pin) were studied in comparison with the hardness of the bone tissue. The extent and morphological features of the injuries to the bones and soft tissues in different parts of the skull were compared with reference to deformation and strength characteristics developing in response to a blow and compression.

  2. A giant abdominal hematoma caused by seatbelt injury led to the development of shock.

    Science.gov (United States)

    Nishino, Tomoya; Morita, Seiji; Sakurai, Keiji; Watanabe, Yasue

    2015-08-01

    Trauma to soft tissues is an important consideration in cases of seatbelt injury, as soft-tissue injury can cause shock. Careful observation for hours in the clinic at least, along with appropriate imaging studies, is necessary if signs of a seatbelt injury exist.

  3. Injury Biomechanics of the Cervical Spine in Car Collisions - some Needs for Further Research

    OpenAIRE

    Svensson, Mats Y

    2003-01-01

    The article reviews some of the existing research concerning the incidence of soft tissue neck injuries sustained in car collisions. A variety of neck injuries, including to muscles, ligaments, facet joints, discs and nerve tissue appear to result from whiplash trauma. Research questions concerning the relationship between these possible injuries and the large set of known whiplash symptoms are considered.

  4. Injury Biomechanics of the Cervical Spine in Car Collisions - some Needs for Further Research

    Directory of Open Access Journals (Sweden)

    Mats Y. Svensson

    2003-01-01

    Full Text Available The article reviews some of the existing research concerning the incidence of soft tissue neck injuries sustained in car collisions. A variety of neck injuries, including to muscles, ligaments, facet joints, discs and nerve tissue appear to result from whiplash trauma. Research questions concerning the relationship between these possible injuries and the large set of known whiplash symptoms are considered.

  5. 间歇性渐变压力模式对大鼠后肢深部组织损伤的影响%The effects of intermittent pressure on deep tissue injuries

    Institute of Scientific and Technical Information of China (English)

    梁兴民; 姬忠良; 段俊峰; 麦艳婷

    2012-01-01

    目的 通过观察间歇性渐变压力模式及持续性压力模式作用大鼠胫骨前肌所引发的灌注障碍及肌纤维损伤程度,从而探讨间歇性渐变压力模式在防治深部组织损伤中的相关机制.方法 共选取10~12周龄SD大鼠20只,采用随机数字表法将其分为实验组及对照组.实验组大鼠胫骨前肌部位给予间歇性渐变压力(压力范围8.0 ~ 21.3 kPa)作用,对照组相同部位则给予持续性压力(压力范围为13.3 kPa)作用,每施压2h则间歇0.5h(为1个周期).于施压3个周期后观察各组大鼠受压部位皮肤及肌肉大体情况,同时采用激光多普勒血流灌注成像系统检测2组大鼠后肢受压点皮肤血液灌注情况;观察完毕后切开皮肤,采用相同方法重复检测2组大鼠胫骨前肌受压点30 min内的动态血液灌注情况;最后于2组大鼠胫骨前肌受压部位取材,进行磷钨酸苏木素染色(PTAH)以观察肌肉损伤情况.结果 待3个周期施压结束后,发现治疗组大鼠受压部位皮肤血流及肌肉灌注情况均显著优于对照组,组间差异均具有统计学意义(P<0.05).随着时间推移,实验组及对照组受压部位血流速度均有增加趋势,并以实验组的增加幅度较显著,与对照组间差异具有统计学意义(P<0.05).对2组大鼠PTAH染色结果比较后发现,实验组大鼠受压部位肌肉损伤程度明显轻于对照组(P<0.05).结论 与持续性压力模式比较,间歇性渐变压力作用能明显减轻压力诱发的组织缺血缺氧及肌组织压力性损伤,对防治深部组织损伤具有重要意义.%Objective To compare the impacts of sustained and intermittent gradient pressure on perfusion disorders and myofiber injuries and to explore the mechanism by which intermittent gradient pressure helps treat deep tissue injury (DTI). Methods Twenty Sprague-Dawley rats 10-12 weeks old were randomly divided into an experimental group and a control group.The tibialis

  6. Effects of neonatal peripheral tissue injury on pain-related behaviors in adult rats%生后发育期外周组织损伤对大鼠成年后痛行为的影响

    Institute of Scientific and Technical Information of China (English)

    李萌萌; 唐家广; 刘瑛辉; 解葵; 郝建华; 陈军

    2013-01-01

    Objective To observe the effects of peripheral tissue injury in the developmental stage of newborn rats on pain-related behaviors in adult rats.Methods SD rats 1,4,7,14,21 and 28 days after birth were selected in the present study (4 litters at each time point and 10 rats per litter).Each litter of rats was randomly divided into injury group (receiving subcutaneous injection of 20μl bee venom) and control group (receiving subcutaneous injection of 20μl normal saline),with 20 in each group,and then raised for 2 months to adulthood.The baseline pain threshold was observed by measuring spontaneous paw flinching reflex,paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT),then 50μl 0.4% bee venom was subcutaneously injected to each rat,and the changes in pain reaction and pain threshold were determined.Results The baseline thermal pain threshold in adult rats receiving bee venom or normal saline at different time points after birth was similar,but baseline mechanical pain threshold in adult rats receiving bee venom at 1,4,7 and 14 days after birth was decreased significantly compared with the adult rats receiving normal saline at corresponding time points(P<0.01).The persistent spontaneous pain response of adult rats in injury group was increased compared with that in control group (P<0.01).No obvious difference in heat hyperalgesia was found between the two groups (P>0.05).Mechanical hyperalgesia was not induced in rats injected with bee venom but induced in adult rats injected with normal saline 4-21 days after birth.Injection of bee venom 21 and 28 days after birth could obviously enhance the bee venom-induced hyperalgesia in adult rats compared with control group (P<0.01).Conclusions Bee venom stimuli at different time points after birth could affect the baseline PWMT and mechanical pain hypersensitivity in adult rats but not the baseline PWTL and thermal pain hypersensitivity.The 21st day maybe a key time point of

  7. Birth Injury

    Science.gov (United States)

    ... Commentary Recent News Scientists Working on Solar-Powered Prosthetic Limbs Exercise a Great Prescription to Help Older Hearts Bavencio Approved for Rare Skin Cancer Older Mothers May Raise Better-Behaved Kids, Study ... lying in an abnormal position in the uterus before birth. Overall, the rate of birth injuries is much lower now than in previous decades because of improved ...

  8. 按摩抑制兔钝挫伤骨骼肌瘢痕形成的机制研究%How massage inhibits scar tissue formation in skeletal muscles after blunt injury

    Institute of Scientific and Technical Information of China (English)

    刘仁建; 唐成林; 邹敏; 郜婕; 谢辉; 陈晓琳

    2012-01-01

    目的 观察按摩对实验兔受损股四头肌转移生长因子(TGF-β1)及Ⅰ型胶原(COL-Ⅰ)mRNA表达的影响,以探讨按摩抑制瘢痕形成的相关机制.方法 共选取健康成年雄性新西兰大白兔40只,采用随机数字表法将其分为正常对照组(4只)、自然恢复组(20只)及按摩组(16只).正常对照组饲养期间未给予特殊处理,采用自制打击器将自然恢复组及按摩组实验兔制成兔右后肢股四头肌损伤模型.按摩组实验兔于制模后第5天时给予按摩治疗,自然恢复组制模后未给予按摩治疗.于制模后7d、11d、15d及19d时采用实时定量RT-PCR法检测各组实验兔TGF-β1,COL-Ⅰ mRNA表达情况.结果 在制模后第7天时,发现按摩组TGF-β1、COL-ImRNA表达量与自然恢复组间差异无统计学意义(P>0.05),在制模后第11,15及19天时,发现按摩组TGF-β1、COL-Ⅰ mRNA表达量均显著低于自然恢复组水平(P<0.05).结论 按摩能显著降低实验兔受损股四头肌TGF-β1及COL-Ⅰ mRNA表达,有助于抑制瘢痕过度形成,从而促进受损肌组织修复.%Objective To investigate the effect of massage on quadriceps femoris repair after injury by external force and the expression of transforming growth factor β1 (TGF-β1) and collagen-Ⅰ (COL-Ⅰ) mRNA.To explore the molecular mechanisms inhibiting scar tissue formation and promoting muscle repair.Methods Forty New Zealand white rabbits weighing (2.0 ±0.5) kg were randomly divided into a normal control group (A) (n =4),a selfrepair group (B) (n =20,further divided into the 3rd,7th,11th,15th and 19th day time points),and a massage group (C) (n =16,further divided as in group B).In group A the rabbits were not treated,as normal controls.In groups B and C rabbit models of quadriceps femoris injury were prepared using a self-made beater.In group B no massage therapy was given as a natural recovery control; in group C,massage therapy was given after 5 days.Realtime quantitative

  9. Skeletal muscle cell apoptosis following motornerve injury versus sensory nerve injury

    Institute of Scientific and Technical Information of China (English)

    Lei Zhao; Ruisheng Xu; Shenyang Jiang; Guangming Lü; Zhiqiang Yan; Junming Sun; Ling Wang; Ye Xue; Donglin Jiang

    2011-01-01

    Skeletal muscle atrophy inevitably occurs in denervated skeletal muscle, and cell apoptosis plays an important role in skeletal muscle atrophy and degeneration. The present study established rat models of simple nerve injury by transecting the ventral or dorsal spinal nerve root and observed rat skeletal muscle cell apoptosis following simple motor nerve injury versus simple sensory nerve injury. Following skeletal muscle denervation for 10 weeks, cell apoptosis was detected in skeletal muscle, which was accompanied by obvious changes in rat behavior and electrophysiological responses. In addition, changes in cross-sectional area and average gray-scale of motor endplates of the gastrocnemius muscle were analyzed following sciatic nerve injury and motor nerve injury.Cell nuclei in denervated skeletal muscle tissue were more densely arranged than in normal skeletal muscle tissue. Cell nuclei were most dense in the sciatic nerve injury group, followed by the motor nerve injury group and the sensory nerve injury group. Fas/Fast expression and the number of apoptotic cells increased in denervated skeletal muscle, and apoptosis-related changes were observed. These findings suggested that motor and sensory nerves provided trophic actions following skeletal muscle and motor nerve injury, resulting in a greater influence on skeletal muscle atrophy than sensory nerve injury. Therefore, reconstruction of motor nerves should be preferentially considered for treating denervation-induced skeletal muscle atrophy.

  10. [Complex pelvic injury in childhood].

    Science.gov (United States)

    Schmal, H; Klemt, C; Haag, C; Bonnaire, F

    2002-08-01

    Pelvic disruptions are rare in children caused by the flexible anchoring of bony parts associated with a high elasticity of the skeleton. Portion of pelvic fractures in infants is lower than 5% even when reviewing cases of specialized centers. The part of complex pelvic injuries and multiple injured patients in infants is higher when compared to adults, a fact caused by the more intense forces that are necessary to lead to pelvic disruption in children. Combination of a rare injury and the capability of children to compensate blood loss for a long time may implicate a wrong security and prolong diagnostic and therapeutic procedures--a problem that definitely should be avoided. Three cases were analyzed and established algorithms for treatment of patients matching these special injury-features demonstrated. A good outcome may only be achieved when all components of injury pattern get recognized and treatment is organized following the hierarchy of necessity. Therefore in the time table first life-saving steps have to be taken and then accompanying injuries can be treated that often decisively influence life quality. As seen in our cases unstable and dislocated fractures require open reduction and internal fixation ensuring nerval decompression, stop of hemorrhage and realizing the prerequisite for effective treatment of soft tissue damage. The acute hemorrhagic shock is one of the leading causes of death following severe pelvic injuries. After stabilization of fracture, surgical treatment of soft tissue injuries and intraabdominal bleeding sources the immediate diagnostic angiography possibly in combination with a therapeutic selective embolization is a well established part of the treatment. The aim of complete restitution can only be accomplished by cooperation of several different specialists and consultants in a trauma center.

  11. Traumatic injuries of the hip.

    LENUS (Irish Health Repository)

    Marshall, Nina

    2009-11-01

    Traumatic lesions of the hip in athletes may be clinically challenging because of the overlap in clinical presentation due to differing pathologies and the presence of multiple injuries. Imaging of the hip in the athlete has undergone a recent resurgence of interest and understanding related to the increasing accessibility and use of hip arthroscopy, which expands the treatment options available for intra-articular pathology. MR imaging and MR arthrography have a unique role in diagnosis of these pathologies, guiding the surgeon, arthroscopist, and referring clinician in their management of bony and soft tissue injury.

  12. Fibronectin in tissue regeneration : timely disassembly of the scaffold is necessary to complete the build

    NARCIS (Netherlands)

    Stoffels, Josephine M. J.; Zhao, Chao; Baron, Wia

    2013-01-01

    Tissue injury initiates extracellular matrix molecule expression, including fibronectin production by local cells and fibronectin leakage from plasma. To benefit tissue regeneration, fibronectin promotes opsonization of tissue debris, migration, proliferation, and contraction of cells involved in th

  13. Treatment of osteochondral injuries with platelet gel

    Directory of Open Access Journals (Sweden)

    Marcus Vinicius Danieli

    2014-12-01

    Full Text Available OBJECTIVES: Treatments for injured articular cartilage have not advanced to the point that efficient regeneration is possible. However, there has been an increase in the use of platelet-rich plasma for the treatment of several orthopedic disorders, including chondral injuries. Our hypothesis is that the treatment of chondral injuries with platelet gel results in higher-quality repair tissue after 180 days compared with chondral injuries not treated with gel. METHODS: A controlled experimental laboratory study was performed on 30 male rabbits to evaluate osteochondral injury repair after treatment with or without platelet gel. Osteochondral injuries were surgically induced in both knees of each rabbit at the medial femoral condyle. The left knee injury was filled with the platelet gel, and the right knee was not treated. Microscopic analysis of both knee samples was performed after 180 days using a histological grading scale. RESULTS: The only histological evaluation criterion that was not significantly different between treatments was metachromasia. The group that was treated with platelet gel exhibited superior results in all other criteria (cell morphology, surface regularity, chondral thickness and repair tissue integration and in the total score. CONCLUSION: The repair tissue was histologically superior after 180 days in the study group treated with platelet gel compared with the group of untreated injuries.

  14. 双极电凝、LigaSure 及超声刀对人子宫旁血管组织热损伤的实验研究%Experimental Study on Thermal Effects of Human Uterine Vascular Tissues by Bipolar Electrocoagulation, LigaSure and ;Ultrasound Knife

    Institute of Scientific and Technical Information of China (English)

    梁军; 邢慧敏; 刘艳; 杨波

    2016-01-01

    Objective To study the thermal damage of uterine vascular tissues by bipolar electrocoagulation , LigaSure and ultrasound knife in total laparoscopic hysterectomy ( TLH ) , and to guide the selection of electeosurgery equipments in TLH . Methods TLH was carried out in patients with myoma of uterus , adenomyosis and dysfunctional uterine bleeding .A total of 90 uterine vascular tissues were studied , including 30 tissues receiving bipolar electrocoagulation , 30 tissues receiving LigaSure electrocoagulation and 30 tissues receiving ultrasound knife dessection .The differences of the thermal damage width of uterine vascular tissues were detected. Results The thermal damage width with bipolar electrocoagulation [(4.583 ±0.538) mm] was significantly greater than the LigaSure electrocoagulation [(4.207 ±0.525) mm, q=3.654, P<0.05] and the ultrasound knife [(3.506 ± 0.623) mm, q=10.465, P<0.05].The thermal damage width with the LigaSure electrocoagulation was significantly greater than the ultrasound knife (q=6.812, P<0.05). Conclusions Bipolar electrocoagulation has the greatest thermal damage width , followed by LigaSure electrocoagulation and ultrasound knife .It is necessary to choose laparoscopic electrosurgical instruments reasonably to avoid side-injury during performance of uterine vessels in TLH .%目的:探讨腹腔镜下全子宫切除术( total laparoscopic hysterectomy , TLH)中双极电凝、LigaSure及超声刀对人子宫旁血管组织的热损伤程度,指导TLH术中合理选择电外科器械。方法对拟行TLH患者截取分别以双极电凝、LigaSure及超声刀处理的子宫旁血管组织各30块,共计90块,显微镜下测量电凝部位热损伤宽度。结果3种电外科器械对子宫旁血管组织热损伤程度不同,热损伤宽度双极电凝组(4.583±0.538)mm,明显大于LigaSure 组(4.207±0.525)mm(q=3.654,P<0.05)和超声刀组(3.506±0.623)mm(q=10.465,P<0.05

  15. [Cold therapy of athletic injuries. Current literature review].

    Science.gov (United States)

    Thorsson, O

    2001-03-28

    Cryotherapy is often used in soft tissue sports injuries. The application of a cold pack reduces local muscular blood-flow by approximately 50% after 10 minutes. The duration of bleeding in a muscular injury is not known, but immediate application of external pressure is probably far superior in emergency treatment of an injury. Some studies have shown significant effect of cryotherapy in emergency treatment of ankle sprains, but external pressure is often applied simultaneously and the additive effect of cryotherapy is therefore uncertain. Cryotherapy reduces the metabolic rate in injured muscle and is often used several days after a soft tissue injury to reduce secondary hypoxic injury. Experimental studies, however, show no effect of cryotherapy on muscle regeneration, and no controlled clinical study has shown a significant effect in emergency treatment of soft tissue sports injuries.

  16. Overuse Injury: How to Prevent Training Injuries

    Science.gov (United States)

    ... http://www.niams.nih.gov/Health_Info/Sports_Injuries/sports_injuries_ff.asp. Accessed Dec. 21, 2015. Tips for ... cfm?topic=A00132. Accessed Dec. 21, 2015. Overuse injury. The American Orthopaedic Society for Sports Medicine. http://www.stopsportsinjuries.org/overuse-injury.aspx. ...

  17. Lower limb landmine injuries.

    Science.gov (United States)

    Necmioglu, S; Subasi, M; Kayikci, C; Young, D B

    2004-04-01

    The medical records of 186 patients seeking treatment for landmine injuries in the authors' region between 1993 and 2001 were evaluated. Of these patients 13 died of accompanying complications. Ten (10) patients with general body trauma and upper limb trauma were excluded from the study. Of 163 patients with lower-limb injuries included in the study, 21 with traumatic amputation underwent surgical amputation at different levels. Patients without traumatic amputation were divided into 2 groups. There were 41 patients (29%) in Group I who were treated by limb salvage procedures. Treatments used in Group I including wound debridement, tendon repair, skin approximation, minimal osteosynthesis, external fixation of long bones and secondary wound coverage. In Group II, there were 101 patients (71%) with primary amputation. Trans-tibial amputation was performed in 52 cases (51.4%), ankle disarticulation in 24 (23.7%), trans-femoral amputation in 9 (8.9%), partial foot amputation in 8 (7.9%), knee disarticulation in 7 (6.9%) and hip disarticulation in 1 case. In Group I, there was infection in 21 patients (51.2%), revision in 27 (65.8%), and amputation in 15 (36.5%). In Group II, there was infection in 28 patients (27.7%), revision in 17 (16.8%), and amputation at a higher level in 8 (7%). In crush injuries such as those resulting from landmines, soft tissue, vascular, and neurological assessment must be performed with utmost care. Even so, the desired success in interventions intended to save a limb is complicated by a high infection rate, soft tissue complications, and high revision amputation rates. Therefore, a decision to amputate in the early term based on an accurate preoperative assessment is crucial.

  18. The Clinical Efficacy of LNG-IUS in the Treatment of Menstrual Abnormalities and Anemia of Adenomyosis%放置曼月乐治疗子宫腺肌病患者月经异常和贫血的疗效分析

    Institute of Scientific and Technical Information of China (English)

    郝茜

    2009-01-01

    目的 探讨曼月乐对子宫腺肌病患者月经异常和贫血的临床效果.方法 对86例子宫腺肌病患者放置曼月乐后进行6个月的随访,在1、2、4、6个月时利用VAS评分法分析患者痛经程度、月经量、经期以及贫血症状情况.结果 患者痛经评分、月经量运月下降,在1、2、4、6个月时,较放置前有明显下降(P<0.05或P<0.01),经期在第6个月时明显缩短(P<0.01);贫血症患者比例逐月降低,较放置前有明显下降(P<0.05或P<0.01).结论 曼月乐是治疗子宫腺肌病的一种较为有效且安全的方法.%OBJECTIVE To investigate the the clinical efficacy of levonorgestrel-releasing intrauterine system(LNG-IUS)in the treatment of adenomyosis. METHODS Eighty six patients with adenomysois were followed up in 0,1,2,4,6 months after treating with LNG-IUS, dysmenorrhea, menstrual blood volume, menstrual period and anemia of the patients. RESULTS Dysmenorrheal scores and menstrual blood volume of the patients decreased gradually after LNG-IUS, it was decreased significantly (P<0.05 or P<0.01 ), when the sixth moth menstrual period of patients was less than it when before LNG-IUS, anemia of adenomyosis was decreased gradually after LNG-IUS, and it were decreased significantly(P<0.05 or P<0.01 ). CONCLUSIONS LIG-IUS is an effective and safe therapy for adenomyosis.

  19. Application analysis of levonorgestrel intrauterine device in treatment of young and middle-aged people with adenomyosis%左炔诺孕酮宫内节育器在中青年子宫腺肌症治疗中的应用分析

    Institute of Scientific and Technical Information of China (English)

    涂小娥; 刘强

    2014-01-01

    目的:探析左炔诺孕酮宫内节育系统在中青年子宫腺肌症治疗中的临床效果。方法选取我院就诊治疗子宫肌腺患者28例子宫腺肌患者,观察在放置左炔诺孕酮宫内节育器前后痛经、月经量、子宫体积、子宫内膜厚度、血清CA125、血清性激素水平的变化情况,比较治疗前后患者临床症状、激素水平的变化及不良反应。结果左炔诺孕酮宫内节育器放置12个月后,患者的痛经程度降低,子宫体积和子宫内膜厚度明显减小,CA125水平明显减低,与放置前相比差异有统计学意义(P<0.05)。结论利用左炔诺孕酮宫内节育器可明显改善子宫腺肌症中痛经、月经量等临床症状,值得临床进一步研究进而推广。%Objective To explore the clinical effects of levonorgestrel intrauterine device in the treatment of young and middle-aged people with adenomyosis.Methods Twenty-eight patients with adenomyosis treated in our hospital were selected.Changes of the patients'dysmenorrhea,menstrual blood volume,uterine volume, endometrial thickness,serum CA125 and serum sex hormone levels after placing the levonorgestrel intrauterine device were observed.The clinical symptoms,hormone level changes and adverse reactions of the patients before and after the placement of levonorgestrel intrauterine device were compared.Results After placing the levonorgestrel intrauterine device for 12 months, the patients'dysmenorrhea degree decreased,uterine volume and endometrial thickness significantly reduced, and CA125 level significantly lowered,with significant differences to those before placing (P<0.05).Conclusion The application of levonorgestrel intrauterine device can significantly improve the clinical symptoms such as dysmenorrhea and menstrual blood volume in adenomyosis,thereby worthy of further clinical study and subsequent promotion.

  20. Plant injury induced by ozone

    Energy Technology Data Exchange (ETDEWEB)

    Hill, A.C.; Pack, M.R.; Treshow, M.; Downs, R.J.; Transtrum, L.G.

    1961-06-01

    Phytotoxicity of ozone to 34 plant species was studied in controlled-atmosphere greenhouses. Plants were subjected at various stages of growth to 0.13-0.72 ppm ozone for 2-hour periods. Injury symptoms developed on 28 species. Some of the most sensitive species were small grains, alfalfa, spinach, and tobacco. There was a general tendency for sensitivity to increase with maturity of tissue. Palisade cells were most readily injured by ozone. On plants with adaxial palisade parenchyma, chlorotic spots and bleached necrotic areas developed on the upper leaf surface. Injury was equally apparent from either leaf surface of plants with undifferentiated mesophyll. Necrotic spots extending completely through the leaf developed on plants with either mesophyll structure when injury was severe. Ozone caused conspicuous tumors to develop on broccoli leaves. Symptoms similar to those produced by ozone fumigations have been observed on a wide range of plant species growing near several large metropolitan centers. 18 references, 8 figures, 2 tables.

  1. Implantation of In Vitro Tissue Engineered Muscle Repair Constructs and Bladder Acellular Matrices Partially Restore In Vivo Skeletal Muscle Function in a Rat Model of Volumetric Muscle Loss Injury

    Science.gov (United States)

    2014-01-01

    Miller, M., Patel, M., Wang, Z., et al. Compression induced muscle injury in rats that mimics compartment syndrome in humans. Am J Pa thol 180, 787, 2012...Bone Joint Surg Am 84-A, 992, 2002. 48. Rhoads, R.P., Johnson, R.M., Rathbone, C.R., Liu, X., Temm Grove, C., Sheehan , S.M., et al. Satellite cell

  2. Mesenchymal Stromal Cell-Derived Factors Promote Tissue Repair in a Small-for-Size Ischemic Liver Model but Do Not Protect against Early Effects of Ischemia and Reperfusion Injury

    NARCIS (Netherlands)

    S.M.G. Fouraschen (Suomi M. G.); J.H. Wolf (Joshua H.); L.J.W. van der Laan (Luc); P.E. de Ruiter (Petra E.); W. Hancock; J.P. Van Kooten (Job P.); M.M.A. Verstegen (Monique); K.M. Olthoff (Kim); J. de Jonge (Jeroen)

    2015-01-01

    textabstractLoss of liver mass and ischemia/reperfusion injury (IRI) are major contributors to postresectional liver failure and small-for-size syndrome. Mesenchymal stromal cell-(MSC-) secreted factors are described to stimulate regeneration after partial hepatectomy. This study investigates if liv

  3. Mechanisms of chronic pain from whiplash injury.

    Science.gov (United States)

    Davis, Charles G

    2013-02-01

    This article is to provide insights into the mechanisms underlying chronic pain from whiplash injury. Studies show that injury produces plasticity changes of different neuronal structures that are responsible for amplification of nociception and exaggerated pain responses. There is consistent evidence for hypersensitivity of the central nervous system to sensory stimulation in chronic pain after whiplash injury. Tissue damage, detected or not by the available diagnostic methods, is probably the main determinant of central hypersensitivity. Different mechanisms underlie and co-exist in the chronic whiplash condition. Spinal cord hyperexcitability in patients with chronic pain after whiplash injury can cause exaggerated pain following low intensity nociceptive or innocuous peripheral stimulation. Spinal hypersensitivity may explain pain in the absence of detectable tissue damage. Whiplash is a heterogeneous condition with some individuals showing features suggestive of neuropathic pain. A predominantly neuropathic pain component is related to a higher pain/disability level.

  4. Tissue tests

    NARCIS (Netherlands)

    Sonneveld, C.; Voogt, W.

    2009-01-01

    Tissue tests are widely used in horticulture practice and have in comparison with soil or substrate testing advantages as well disadvantages in comparison with soil testing. One of the main advantages of tissue tests is the certainty that analysed nutrients in plant tissues are really present in the

  5. 基于文献挖掘的高氧损伤肺组织相关基因的生物信息学分析%Literature and bioinformatic analysis of dysregulated genes in lung tissues of hyperoxic lung injury

    Institute of Scientific and Technical Information of China (English)

    黄宇戈; 张山丹; 韩浩; 陈星; 殷海平; 郑学辉

    2013-01-01

    Objective To explore the pathogenesis mechanism of hyperoxic lung injury and the effective means for its clinical treatment,the difference of the gene expressions between lung tissues of hyperoxic lung injury and normal lung was compared.Methods The differentially expressed genes between lung tissues of hyperoxic lung injury and normal lung were obtained from PubMed.The dysregulated genes in lung tissues of hyperoxic lung injury were analyzed by a series of bioinformatics methods,including pathways,gene ontology and functional annotation clustering analysis.Results 467 lines of differentially expressed genes were found and genes more than 2-fold regulated were 189.We sought the mapping of genes in the KEGG databases through functional annotation tools,and we discovered there were 5 lines of pathways with difference having outstangding statistical significance through metabolic pathways enrichment degree analysis.It reflected the pathways were closely related to hyperoxic lung injury (the 2-fold upregulated genes were 14,the 2-fold down-regulated genes were 6).GO analysis revealed that these genes were involved in hematopietic cell lineage,axon guidance,adherens junction,T cell receptor signaling pathway and focal adhesion.Conclusions Therefore,it is believed that the above-mentioned 20 lines of gnes are the major ones for the hyperoxic lung injury and the research on them will provide effective means for revealing the molecular mechanism of hyperoxic lung injury and identifying the targeted therapy.%目的 比较小鼠高氧肺损伤肺组织与正常肺组织的差异表达基因,从分子水平揭示高氧肺损伤的发病机制.方法 利用PubMed数据库获取小鼠高氧肺损伤肺组织与正常肺组织的差异表达基因,并进行生物学通路、基因本体和功能注释聚类等生物信息分析.结果 两组间差异表达基因共467条,以2倍标准筛选出189条差异表达基因,再用功能注释工具将超过2倍差异表达的基因

  6. Acute toxicity and tissue injury studies of ivermectin for Songpu mir-ror carp (Cyprinus specularis songpu)%伊维菌素对松浦镜鲤的急性毒性与组织损伤

    Institute of Scientific and Technical Information of China (English)

    韩冰; 王荻; 卢彤岩

    2015-01-01

    分别以1.30 mg/kg、1.69 mg/kg、2.19 mg/kg、2.86 mg/kg、3.71 mg/kg、4.83 mg/kg、6.27 mg/kg、8.16 mg/kg剂量的伊维菌素(ivermectin)对松浦镜鲤(Cyprinus specularis songpu)进行口灌,并设置空白对照和溶剂对照,给药后连续观察96 h,记录各组鱼的行为和死亡情况。用 SPPS 软件计算得出松浦镜鲤口灌伊维菌素的96 h LD50为4.04 mg/kg,95%可信区间为3.42~4.80 mg/kg。对给药组和对照组鱼进行血细胞分析,发现随着给药剂量的增加,红细胞数无明显变化,血红蛋白浓度呈上升趋势,而白细胞数呈下降趋势。通过组织切片观察,发现伊维菌素给药剂量为4.83 mg/kg 时松浦镜鲤体内组织开始出现明显的病理变化。大剂量口灌伊维菌素后,松浦镜鲤肠绒毛出现坏死、脱落现象,肝细胞萎缩、肝血窦变大,肾小管上皮细胞变性、坏死,心肌纤维萎缩、变性。本研究通过探讨伊维菌素对松浦镜鲤的毒性效应,旨在为松浦镜鲤养殖过程中科学合理的使用伊维菌素提供基础数据和科学依据。%In order to evaluate the toxic effects of ivermectin for Songpu mirror carp, the acute toxicity and tissue injury of ivermectin for Songpu mirror carp was studied. Ivermectin was given by oral administration to Songpu mirror carp at the dosage of 1.30 mg/kg, 1.69 mg/kg, 2.19 mg/kg, 2.86 mg/kg, 3.71 mg/kg, 4.83 mg/kg, 6.27 mg/kg, 8.16 mg/kg re-spectively. Blank control and solvent control were sited also. After given ivermectin, the fish were investigated in 96 h;the dead fish were sacrificed at once. Through calculating by SPSS, the 96h LD50 and 95% confidence of LD50 of iver-mectin for Songpu mirror carp were 4.04 mg/kg and 3.42~4.80 mg/kg, respectively. The results show that with the in-crease of the dose, the number of red blood cells had no obvious change, the hemoglobin concentration had rised and the white blood cell count had decreased, according to analysis blood cell of the administration

  7. An Unusual Ankle Injury: The Bosworth-Pilon Fracture.

    Science.gov (United States)

    Peterson, Nicholas D; Shah, Feisal; Narayan, Badri

    2015-01-01

    The Bosworth injury occurs when the distal fibula becomes entrapped posterior to the posterior tibial tubercle, usually as a result of a supination external rotation injury. This uncommon occurrence is a recognized cause of an irreducible ankle dislocation. A pilon fracture is usually a high-energy injury caused by the talus being driven upward into the tibial plafond. The resulting bone and soft tissue injuries often require a staged approach to management. The present report is the first in the medical data to describe a Bosworth injury complicating a pilon fracture. We also discuss a management approach for this rare fracture.

  8. Low intensity laser treatment of nerve injuries

    Science.gov (United States)

    Liu, Xiao-Guang; Liu, Timon Cheng-Yi; Luo, Qing-Ming

    2007-05-01

    The neural regeneration and functional recovery after nerve injuries has long been an important field in neuroscience. Low intensity laser (LIL) irradiation is a novel and useful tool for the treatment of many injuries and disorders. The aim of this study was to assess the role of LIL irradiation in the treatment of peripheral and central nerve injuries. Some animal experiments and clinical investigations have shown beneficial effects of LIL irradiation on neural tissues, but its therapeutic value and efficacy are controversial. Reviewing the data of experimental and clinical studies by using the biological information model of photobiomodulation, we conclude that LIL irradiation in specific parameters can promote the regeneration of injured peripheral and central nerves and LIL therapy is a safe and valuable treatment for superficial peripheral nerve injuries and spinal cord injury. The biological effects of LIL treatment depend largely on laser wavelength, power and dose per site and effective irradiation doses are location-specific.

  9. Observation of the curative effect of mifepristone combined with infertility caused left levonorgestrel releasing intrauterine system in acetone in treatment of adenomyosis%米非司酮联合左炔诺孕丙酮宫内缓释系统治疗子宫腺肌病导致不孕症疗效观察

    Institute of Scientific and Technical Information of China (English)

    施振娥

    2013-01-01

    目的:观察米非司酮联合左炔诺孕丙酮缓释系统(LNG-IUS)治疗子宫腺肌病导致不孕症的临床疗效。方法将本院2010年5月~2011年8月收治的子宫腺肌病导致的不孕症患者60例,随机分成治疗组和对照组两组。治疗组:予以米非司酮25mg,每日一次口服,连服3个月。并且所有患者均在签署知情同意书后自愿使用左炔孕丙酮宫内缓释系统。经彩超检查后,由专人于月经第3~7天在用药同时放置左炔诺孕丙酮宫内缓释系统。6个月后取出。对照组:单独给予米非司酮25mg,每日一次口服,连服3个月。观察停药后1、3、6、12个月的怀孕成功率。结果随访至12个月时统计,治疗组平均受孕率79%,对照组平均受孕率57%,治疗组总有效率明显高于对照组(P<0.05)。结论使用米非司酮联合左炔诺孕丙酮宫内缓释系统治疗子宫腺肌病可以明显提高患者的受孕率,对于子宫腺肌病导致的不孕症疗效好于其他方法,而且经济、安全,便于患者接受。%Objective To observe the curative effect of mifepristone combined with infertility caused left levonorgestrel releasing intrauterine system in acetone in treatment of adenomyosis. Methods 60 infertility patients in our hospital from 2010 May to 2011 August were adenomyosis in patients, randomly divided into treatment group and control group two groups.Treatment group was given mifepristone 25mg orally, once a day, and even served three months. And all the patients after informed consent voluntary use of levonorgestrel releasing intrauterine system in acetone, by color Doppler ultrasound examination, by hand in 3-7 days in the menstrual medication at the same time, place left levonorgestrel releasing intrauterine system in acetone, removed after 6 months. Control group was given mifepristone 25mg orally, once a day, and even served three months. The pregnancy, 3, 6, 1 and 12 months were

  10. Gunshot wounds: epidemiology, wound ballistics, and soft-tissue treatment.

    Science.gov (United States)

    Dougherty, Paul J; Najibi, Soheil; Silverton, Craig; Vaidya, Rahul

    2009-01-01

    The extremities are the most common anatomic location for gunshot wounds. Because of the prevalence of gunshot injuries, it is important that orthopaedic surgeons are knowledgeable about caring for them. The most common injuries seen with gunshot wounds are those of the soft tissues. Nonsurgical management of patients who have gunshot wounds with minimal soft-tissue disruption has been successfully accomplished in emergency departments for several years; this includes extremity wounds without nerve, intra-articular, or vascular injury. Stable, nonarticular fractures of an extremity have also been successfully treated with either minimal surgical or nonsurgical methods in the emergency department. Indications for surgical treatment include unstable fractures, intra-articular injuries, a significant soft-tissue injury (especially with skin loss), vascular injury, and/or a large or expanding hematoma.

  11. Brain injury - discharge

    Science.gov (United States)

    ... and caregivers. Biausa.org. www.biausa.org/brain-injury-family-caregivers.htm#Manage the Home . Accessed December 8, 2016. ... Caregiver Alliance; National Center on Caregiving. Traumatic brain injury. ... www.caregiver.org/traumatic-brain-injury . Accessed ...

  12. Growth Plate Injuries

    Science.gov (United States)

    ... Growth Plate Injuries? Key Words Information Box The Salter-Harris Classification of Growth Plate Injuries What Is ... of Growth Plate Injuries? Since the 1960s, the Salter-Harris classification, which divides most growth plate fractures ...

  13. Dealing with Sports Injuries

    Science.gov (United States)

    ... for falling accidents, such as horseback riding and gymnastics. Head injuries include fractures, concussions , contusions (bruises), and ... hockey, or in weightlifting, rowing, golf, figure skating, gymnastics, and dancing. Sex Organ Injuries Injuries to the ...

  14. Traumatic Brain Injury

    Science.gov (United States)

    Traumatic brain injury (TBI) happens when a bump, blow, jolt, or other head injury causes damage to the brain. Every year, millions of people in the U.S. suffer brain injuries. More than half are bad enough that ...

  15. "Floating shoulder" injuries.

    Science.gov (United States)

    Heng, Kenneth

    2016-12-01

    "Floating shoulder" is a rare injury complex resulting from high-energy blunt force trauma to the shoulder, resulting in scapulothoracic dissociation. It is commonly associated with catastrophic neurovascular injury. Two cases of motorcyclists with floating shoulder injuries are described.

  16. Eye Injuries in Sports

    Science.gov (United States)

    ... in Sports Which sports cause the most eye injuries?Sports cause more than 40,000 eye injuries each ... and racquet sports.When it comes to eye injuries, sports can be classified as low risk, high risk ...

  17. Preventing Knee Injuries

    Science.gov (United States)

    ... Our Newsletter Donate Blog Skip breadcrumb navigation Preventing Knee Injuries Knee injuries in children and adolescent athletes ... this PDF Share this page: WHAT ARE COMMON KNEE INJURIES? Pain Syndromes One of the most common ...

  18. Nanomedicine for treating spinal cord injury

    Science.gov (United States)

    Tyler, Jacqueline Y.; Xu, Xiao-Ming; Cheng, Ji-Xin

    2013-09-01

    Spinal cord injury results in significant mortality and morbidity, lifestyle changes, and difficult rehabilitation. Treatment of spinal cord injury is challenging because the spinal cord is both complex to treat acutely and difficult to regenerate. Nanomaterials can be used to provide effective treatments; their unique properties can facilitate drug delivery to the injury site, enact as neuroprotective agents, or provide platforms to stimulate regrowth of damaged tissues. We review recent uses of nanomaterials including nanowires, micelles, nanoparticles, liposomes, and carbon-based nanomaterials for neuroprotection in the acute phase. We also review the design and neural regenerative application of electrospun scaffolds, conduits, and self-assembling peptide scaffolds.

  19. Suicide bomb attack causing penetrating craniocerebral injury

    Institute of Scientific and Technical Information of China (English)

    Manzar Hussain; Muhammad Ehsan Bari

    2013-01-01

    Penetrating cerebral injuries caused by foreign bodies are rare in civilian neurosurgical trauma,although there are various reports of blast or gunshot injuries in warfare due to multiple foreign bodies like pellets and nails.In our case,a 30-year-old man presented to neurosurgery clinic with signs and symptoms of right-sided weakness after suicide bomb attack.The skull X-ray showed a single intracranial nail.Small craniotomy was done and the nail was removed with caution to avoid injury to surrounding normal brain tissue.At 6 months' follow-up his right-sided power improved to against gravity.

  20. A new zebrafish bone crush injury model

    Directory of Open Access Journals (Sweden)

    Sara Sousa

    2012-07-01

    While mammals have a limited capacity to repair bone fractures, zebrafish can completely regenerate amputated bony fin rays. Fin regeneration in teleosts has been studied after partial amputation of the caudal fin, which is not ideal to model human bone fractures because it involves substantial tissue removal, rather than local tissue injury. In this work, we have established a bone crush injury model in zebrafish adult caudal fin, which consists of the precise crush of bony rays with no tissue amputation. Comparing these two injury models, we show that the initial stages of injury response are the same regarding the activation of wound healing molecular markers. However, in the crush assay the expression of the blastema marker msxb appears later than during regeneration after amputation. Following the same trend, bone cells deposition and expression of genes involved in skeletogenesis are also delayed. We further show that bone and blood vessel patterning is also affected. Moreover, analysis of osteopontin and Tenascin-C reveals that they are expressed at later stages in crushed tissue, suggesting that in this case bone repair is prolonged for longer than in the case of regeneration after amputation. Due to the nature of the trauma inflicted, the crush injury model seems more similar to fracture bone repair in mammals than bony ray amputation. Therefore, the new model that we present here may help to identify the key processes that regulate bone fracture and contribute to improve bone repair in humans.

  1. Injuries in orienteering.

    Science.gov (United States)

    Linde, F

    1986-09-01

    In a one-year prospective study of 42 elite orienteers, 73 recent injuries (1.7 per runner per year) were found. Acute injuries totalled 52% and 48% were due to overuse. Ankle sprains made up 37% of acute injuries while the remaining were mainly contusions caused by falls or bumps against branches or rocks. Medial shin pain, Achilles peritendinitis, peroneal tenosynovitis and iliotibial band friction syndrome were the most frequent overuse injuries. All overuse injuries were located in the lower extremity while 18% of acute injuries was located elsewhere. Acute injuries were most frequent in the competitive season while overuse injuries occurred most often during the continuous training period.

  2. Equine-associated maxillofacial injuries: retrospective 5-year analysis.

    Science.gov (United States)

    Islam, Shofiq; Gupta, Benjamin; Taylor, Christopher J; Chow, Jeffrey; Hoffman, Gary R

    2014-02-01

    We explored the relation between the causes of facial injuries in equestrians and the presence or absence of associated injuries. Over a 5-year period we retrospectively reviewed all patients who presented to the John Hunter Hospital, New South Wales, with facial injuries that had resulted from activity with horses. We analysed the rates of hard and soft tissue injuries, and of associated injuries by sex and mechanism. A total of 85 patients were included (50 female and 35 male) with an age range of 2-88 years. There was a significant difference in the rate of maxillofacial and associated injuries when groups were analysed for sex and mechanism of injury. Facial injuries caused by falling from a horse were more often associated with other injuries in men than in women (p<0.05), and men were 4 times more likely to present with associated injuries than women (OR 3.9; 95% CI 1.1 to 14) We also found significant differences in the rates of facial fracture. Women who had been kicked by a horse were more likely to sustain bony injuries than men (p<0.05). Our data confirm the association between kicks and facial fracture, and this may provide an impetus for the development of appropriate protective equipment. Patients who sustain facial injuries when falling from a horse often present with associated injuries and this has practical implications for clinicians involved in their management.

  3. Sports injuries of the ear.

    Science.gov (United States)

    Wagner, G A

    1972-07-01

    The author describes common sports injuries involving the ear. Such injuries include hematoma, lacerations, foreign bodies (tattoo), and thermal injuries. Ear canal injuries include swimmer's ear and penetrating injuries. Tympanum injuries include tympanic membrane perforations, ossicular discontinuity, eustachian tube dysfunction, temporal bone fractures and traumatic facial nerve palsy. Inner ear injuries include traumatic sensorineural deafness. The author emphasizes the management of these injuries.

  4. Pediatric hand treadmill injuries.

    Science.gov (United States)

    Banever, Gregory T; Moriarty, Kevin P; Sachs, Barry F; Courtney, Richard A; Konefal, Stanley H; Barbeau, Lori

    2003-07-01

    The great popularity of physical fitness in modern society has brought many pieces of exercise equipment into our homes for convenience and privacy. This trend has come with an increasing rate of injuries to children who curiously touch moving parts, including treadmill belts. Experience with a recent series of treadmill contact burns to children's hands is described in this article. A retrospective chart review at a tertiary referral center from June 1998 until June 2001 found six children sustaining hand burns from treadmills. The patients' ages at presentation ranged from 15 to 45 months (average of 31 months, three boys and three girls). All injuries occurred in the home while a parent was using the treadmill. Burns involved the palmar aspect of the hand, mostly confined to the fingers, and the severity ranged from partialto full-thickness burns. All patients were initially managed with collagenase and bacitracin zinc/polymyxin B powder dressings to second- and third-degree burns, along with splinting and range-of-motion exercises. Two patients required skin grafting at 2 weeks and 2 months for full-thickness tissue loss and tight joint contracture, respectively. At an average follow-up of 12 months, all patients had full range of motion and no physical limitation. The rate of children injured by exercise equipment is expected to increase. Friction burns to the hands remain a concern, although early recognition and appropriate management are associated with excellent functional outcomes. Protective modification of exercise machines seems to be the best approach to eliminating these injuries.

  5. Infection of Atlantic salmon with Moritella viscosus compared to a mechanical tissue injury model in rainbow trout show similar expression patterns of cytokine genes and may be related to triggering of the same signaling pathways

    DEFF Research Database (Denmark)

    was sampled from infected fish at 4, 7 and 14 days post infection. Samples were obtained from site of lesions and from locations without clinical signs of disease and lesions. To compare the inflammatory reactions from infected fish relative to sterile, mechanical tissue damage, rainbow trout (Oncorhynchus......Physical damage of tissue and multiple kinds of infections are found to cause inflammatory reactions in mammals. Regardless of the difference between non-pathogenic induced tissue damage and a bacterial infection, many of the same pathways and genes are triggered. To determine if the same...... phenomenon occurs in salmonid fishes, Atlantic salmon (Salmo salar) were infected with the bacteria Moritella viscosus, the causative agent of winter ulcer. The clinical signs showing visible, punctual lesions in the skin make this pathogen unique in order to study local inflammation. Muscle tissue...

  6. 烟雾吸入性损伤大鼠肺组织丝裂原活化蛋白激酶通路的变化%Study on activation of mitogen-activated protein kinase pathway in lung tissue of rats with smoke inhalation injury

    Institute of Scientific and Technical Information of China (English)

    安辉; 孙磊; 周燕虹; 曹佳

    2008-01-01

    目的 了解烟雾吸入性损伤大鼠肺组织丝裂原活化蛋白激酶(MAPK)通路及炎性细胞因子含量的变化,探讨其损伤机制. 方法建立密闭舱内烟雾吸入性损伤模型,将30只SD大鼠分为烟雾吸入性损伤后1、6、24、72 h及7 d组,另设正常对照组(6只).取各组大鼠肺组织行病理学观察,检测肺组织匀浆液中肿瘤坏死因子α(TNF-α)、巨噬细胞炎性蛋白2(MIP-2)和白细胞介素1β(IL-1β)含量,用蛋白质印迹法检测肺组织p38MAPK、c-Jun氨基末端激酶(JNK)、细胞外信号调节激酶1/2(ERK1/2)及各酶磷酸化水平.收集大鼠支气管肺泡灌洗液(BALF),检测TNF-α、MIP-2、IL-1β含量并行粒细胞分类、计数. 结果烟雾吸入使大鼠产生急性肺损伤样病理改变.伤后1 h组大鼠肺组织及BALF中TNF-α和IL-1β含量均高于正常对照组(P0.05),伤后1 h组BALF中MIP-2水平高于正常对照组(P0.05).结论 密闭舱室内非金属材料燃烧释放的毒性气体能诱导肺组织产生明显的炎性反应,激活细胞MAPK通路中重要激酶的表达,这可能是毒性混合气体导致肺损伤的重要机制之一.%Objective To investigate the activation of mitogen-activated protein kinase(MAPK) path- way and the expression of inflammatory cytokine in lung tissue of rats with smoke inhalation injury, and to explore the injury mechanism. Methods The model of smoke inhalation injury in airtight cabin was estab- lished. Thirty-six SD rats were randomly divided into normal control group( n = 6,NC group) and inhalation injury group (n = 30, Ⅱ group). The rats in Ⅱ group were observed at 1,6,24,72 post injury hour(PIH) and 7 post injury day(PID). The pathological changes in lung tissue were observed by optical microscope. The contents of TNF-α, MIP-2, IL-1β in lung tissue homogenate were examined. The level of p38MAPK, JNK, ERK1/2 and their phosphorylation in lung tissue were measured by Western blotting. The contents of TNF-α, MIP-2,and

  7. Imatinib enhances functional outcome after spinal cord injury.

    Directory of Open Access Journals (Sweden)

    Mathew B Abrams

    Full Text Available We investigated whether imatinib (Gleevec®, Novartis, a tyrosine kinase inhibitor, could improve functional outcome in experimental spinal cord injury. Rats subjected to contusion spinal cord injury were treated orally with imatinib for 5 days beginning 30 minutes after injury. We found that imatinib significantly enhanced blood-spinal cord-barrier integrity, hindlimb locomotor function, sensorimotor integration, and bladder function, as well as attenuated astrogliosis and deposition of chondroitin sulfate proteoglycans, and increased tissue preservation. These improvements were associated with enhanced vascular integrity and reduced inflammation. Our results show that imatinib improves recovery in spinal cord injury by preserving axons and other spinal cord tissue components. The rapid time course of these beneficial effects suggests that the effects of imatinib are neuroprotective rather than neurorestorative. The positive effects on experimental spinal cord injury, obtained by oral delivery of a clinically used drug, makes imatinib an interesting candidate drug for clinical trials in spinal cord injury.

  8. Neuroprotective effects of vagus nerve stimulation on traumatic brain injury.

    Science.gov (United States)

    Zhou, Long; Lin, Jinhuang; Lin, Junming; Kui, Guoju; Zhang, Jianhua; Yu, Yigang

    2014-09-01

    Previous studies have shown that vagus nerve stimulation can improve the prognosis of traumatic brain injury. The aim of this study was to elucidate the mechanism of the neuroprotective effects of vagus nerve stimulation in rabbits with brain explosive injury. Rabbits with brain explosive injury received continuous stimulation (10 V, 5 Hz, 5 ms, 20 minutes) of the right cervical vagus nerve. Tumor necrosis factor-α, interleukin-1β and interleukin-10 concentrations were detected in serum and brain tissues, and water content in brain tissues was measured. Results showed that vagus nerve stimulation could reduce the degree of brain edema, decrease tumor necrosis factor-α and interleukin-1β concentrations, and increase interleukin-10 concentration after brain explosive injury in rabbits. These data suggest that vagus nerve stimulation may exert neuroprotective effects against explosive injury via regulating the expression of tumor necrosis factor-α, interleukin-1β and interleukin-10 in the serum and brain tissue.

  9. Neuroprotective effects of vagus nerve stimulation on traumatic brain injury

    Science.gov (United States)

    Zhou, Long; Lin, Jinhuang; Lin, Junming; Kui, Guoju; Zhang, Jianhua; Yu, Yigang

    2014-01-01

    Previous studies have shown that vagus nerve stimulation can improve the prognosis of traumatic brain injury. The aim of this study was to elucidate the mechanism of the neuroprotective effects of vagus nerve stimulation in rabbits with brain explosive injury. Rabbits with brain explosive injury received continuous stimulation (10 V, 5 Hz, 5 ms, 20 minutes) of the right cervical vagus nerve. Tumor necrosis factor-α, interleukin-1β and interleukin-10 concentrations were detected in serum and brain tissues, and water content in brain tissues was measured. Results showed that vagus nerve stimulation could reduce the degree of brain edema, decrease tumor necrosis factor-α and interleukin-1β concentrations, and increase interleukin-10 concentration after brain explosive injury in rabbits. These data suggest that vagus nerve stimulation may exert neuroprotective effects against explosive injury via regulating the expression of tumor necrosis factor-α, interleukin-1β and interleukin-10 in the serum and brain tissue. PMID:25368644

  10. Distal radioulnar joint injuries

    Directory of Open Access Journals (Sweden)

    Binu P Thomas

    2012-01-01

    Full Text Available Distal radioulnar joint is a trochoid joint relatively new in evolution. Along with proximal radioulnar joint , forearm bones and interosseous membrane, it allows pronosupination and load transmission across the wrist. Injuries around distal radioulnar joint are not uncommon, and are usually associated with distal radius fractures,fractures of the ulnar styloid and with the eponymous Galeazzi or Essex_Lopresti fractures. The injury can be purely involving the soft tissue especially the triangular fibrocartilage or the radioulnar ligaments.The patients usually present with ulnar sided wrist pain, features of instability, or restriction of rotation. Difficulty in carrying loads in the hand is a major constraint for these patients. Thorough clinical examination to localize point of tenderness and appropriate provocative tests help in diagnosis. Radiology and MRI are extremely useful, while arthroscopy is the gold standard for evaluation. The treatment protocols are continuously evolving and range from conservative, arthroscopic to open surgical methods. Isolated dislocation are uncommon. Basal fractures of the ulnar styloid tend to make the joint unstable and may require operative intervention. Chronic instability requires reconstruction of the stabilizing ligaments to avoid onset of arthritis. Prosthetic replacement in arthritis is gaining acceptance in the management of arthritis.

  11. Tissue types (image)

    Science.gov (United States)

    There are 4 basic types of tissue: connective tissue, epithelial tissue, muscle tissue, and nervous tissue. Connective tissue ... and binds them together (bone, blood, and lymph tissues). Epithelial tissue provides a covering (skin, the linings of ...

  12. Assessment of electrical burn injury using structured illumination in an in-vivo electrical injury model

    Science.gov (United States)

    Nguyen, Thu T. A.; Basiri, Ali; Shupp, J. W.; Moffatt, L. T.; Jordan, M. H.; Jeng, J. C.; Leto, E.; Ramella-Roman, J. C.

    2011-03-01

    Electrical injury is a devastating and hard to treat clinical lesion. Fully understanding the pathophysiology of electrical trauma is still a challenge for clinicians and scientists. Further elucidating the natural history of this form of tissue injury could be helpful in improving limb salvage and offering stage-appropriate therapy. Multi-spectral imaging technique is a non-invasive technology that can be used to determine optical properties of tissues in and adjacent to injury. Images at different wavelengths can provide essential information related to the pathophysiological condition of the tissue. To promote the applicability of this technique in medical diagnosis, we built a complete experimental model of electrical injury. In this model, electrical injuries were created by a reliable high-tension shock system at preset voltage or current. A thermal camera recorded the change of skin temperature during the electrical shock. Then, a high-resolution spectral imaging system based on structured illumination was used to capture images for post analysis to extrapolate optical properties of the tissue. To test accuracy, this imaging system was calibrated by using a set of epoxy phantoms with known optical properties. In this paper, the results of experiments conducted on rats and discussions on the systemic changes in tissue optical properties before and after electrical shock are presented.

  13. 传统中成药治疗气滞血瘀型急性软组织损伤疗效观察%Observation of therapeutic effect of traditional Chinese medicine in treatment of Qi stagnation blood stasis-type acute soft tissue injury

    Institute of Scientific and Technical Information of China (English)

    梁高峰; 乔景泉; 张向阳; 陆琴

    2014-01-01

    Objective:To evaluate the effectiveness and safety of traditional Chinese medicine in treatment of Qi stagnation and blood stasis-type acute soft tissue injury. Methods:82 patients with acute soft tissue injury were randomly divided into treatment group (42 patients) and control group (40 patients). Based on the routine nursing care, the patients in treatment group were given Dahuoluo capsules while those in control group were treated with Xuefu Zhuyu tablets. The treatment of both groups continued for 7days, and then patients'clinical symptoms and physical signs were observed. Results: After the treatment, the scores of pain, swelling and ecchy-mosis of treatment group were lower than those of control group, and the differences had the statistical significance (P<0. 05). Conclu-sions: Traditional Chinese Medicine is effective and safe for the treatment of acute soft tissue injury, and it is worthy of clinical use.%目的:评价传统中药治疗气滞血瘀型急性软组织损伤的疗效及安全性。方法:按入院顺序,将82例气滞血瘀型急性软组织损伤患者随机分为治疗组42例和对照组40例,按损伤程度给予常规护理的基础上分别给予大活络胶囊和血府逐瘀片治疗,7d 后观察患者治疗前、后的临床症状、体征变化情况。结果:治疗后患者的疼痛、肿胀、瘀斑积分等明显下降,治疗组明显优于对照组,总疗效对比有统计学意义(P<0.05)。结论:中医治疗活动期风湿性关节炎疗效明显、安全,值得临床推广使用。

  14. 水飞蓟素对急性肺损伤小鼠肺组织IL-1β、IL-6、趋化因子fractalkine表达的影响%The effects of silymarin on IL-1β, IL-6, fractalkine gene expression in lung tissue rats with acute lung injury

    Institute of Scientific and Technical Information of China (English)

    张晓鸣; 顾绍庆

    2012-01-01

    Objective:To investigate the effects of silymarin (SIL) on the expressions of IL-lβ, IL-6,and fractalkine in lung tissue of rat with acute lung injury. Methods; A rat model of acute lung injury (ALI) was induced by lipopolysaecharide (LPS). All rats were randomly divided into SIL group, LPS group and normal saline control group. SIL(200 mg/kg) was dropped into airway of rats in SIL group 6,4,and 2 h before treated with LPS. After LPS was given for 6,12 and 24 h,IL-lβ,IL-6,chemotatic factor fractalkine protein levels in lung tissue homogenate were detected by ELISA. Results:At all time point,IL-lβ,IL-6,fractalkine protein levels of SIL group were all significantly lower than thoBe in LPS group (P < 0.05). Conclusion:SIL could significantly inhibit the expression of IL-lβ,IL-6,fractalkine in lung tissue of rat with acute lung injury.%目的:探讨水飞蓟素(silymarin,SIL)对急性肺损伤小鼠肺组织中白细胞介素(interleukin,IL)-1β、IL-6、趋化因子fractalkine表达的影响与意义.方法:利用内毒素(lipopolysaccharide,LPs)气管内滴入制备小鼠急性肺损伤的模型;设立SIL治疗组(S组)、LPS组(I组)、生理盐水对照组(N组),S组在气管内滴入LPS前6、4、2h以200 mg/kg水飞蓟素灌胃,分别在LPS处理后6、12、24 h取肺组织匀浆,用ELISA法分别测定各组IL-1β、IL-6、fractalkine蛋白水平.结果:在各时间点,S组肺组织中IL-1β、IL-6、fractalkine蛋白表达水平均显著低于L组.结论:SIL能有效抑制小鼠急性肺损伤时肺组织中IL-1β、IL-6、趋化因子fractalkine的表达.

  15. The model of uterine adenomyosis in mice induced by tamoxifen and the effect on fertility%子宫腺肌病小鼠模型建立及对生育力的影响

    Institute of Scientific and Technical Information of China (English)

    夏丽群; 朱海斌; 夏雅仙

    2013-01-01

    目的:使用口服他莫西芬法建立美国癌症研究所( ICR)小鼠子宫腺肌病模型,并对此模型生育力情况进行评估。方法取24只新生的雌性ICR小鼠,于日龄第2天时开始口服他莫西芬,于日龄42天、90天时分别进行子宫组织形态学观察,于日龄90天时与正常性成熟雄鼠进行交配,妊娠第9天取出子宫组织,剖腹观察双侧子宫胚胎着床数。结果①子宫腺肌病模型小鼠在42日龄时出现子宫内膜间质浸润,肌层发育紊乱;②模型小鼠于90日龄时出现子宫腺肌病典型表现:子宫内膜侵入肌层,但尚未达浆膜下;③体内试验发现,与正常对照小鼠相比,模型小鼠生育力明显下降(Fisher’s值为0.000,P<0.01)。结论口服他莫西芬法可成功诱导ICR小鼠子宫腺肌病模型,此模型小鼠生育力下降,可作为生殖医学领域子宫腺肌病研究较好的动物模型。%Objective To establish adenomyosis ( AM) model of mice by oral administration of tamoxifen to Institute of Cancer Research ( ICR) mice, and to study the fertility of the AM model .Methods Twenty-four newly born ICR mice with AM were induced by oral administration of tamoxifen from day 2 after birth.The histomorphology of uterus on day 42 and 90 was observed .The models of mice with AM were mated to normal male mice on day 90, and the implantation of embryos in uterus on 9th pregnant day was observed .Results There was infiltration of stroma in the endometrium and disordered development in tunica muscularis in the uterus of model mice on day 42. There were typical manifestations of AM in model mice on day 90, endometrium infiltrating muscular layer but not reaching the subserosa . In vivo experiment, a significantly declining of fertility in AM model mice was found when they were compared with control mice (Fisher’ s value was 0.000, P<0.01).Conclusion Oral administration of tamoxifen can induce the mouse model of

  16. The treatment of sports injuries by local injection.